title: HMGB1 in ischemic and non-ischemic liver after selective warm ischemia/reperfusion in rat.
abstract: High mobility group box 1 (HMGB1) acts as an early mediator in inflammation and organ injury. Ischemia reperfusion (I/R) injury induces HMGB1 translocation and expression in ischemic areas. However, it is unknown whether selective warm liver I/R injury also induces the expression of HMGB1 in non-ischemic lobes. The present study aimed to test the hypothesis that selective liver I/R injury also causes HMGB1 translocation and up-regulates its expression in non-ischemic liver areas. In the present study, selective I/R injury was induced by clamping the median and left lateral liver lobes for 90 min followed by 0.5, 6 and 24 h reperfusion. We used male inbred Lewis rats; six animals for each point in time and six animals for the normal control group. Selective hepatic I/R injury induced morphological changes not only in ischemic lobes but also in non-ischemic lobes. HMGB1 translocation and expression was increased in a time-dependent manner in the ischemic lobes, and increased in with delayed onset in the non-ischemic lobes. Serum HMGB1 levels were increased after reperfusion. Furthermore, liver I/R injury up-regulated the expression of HMGB1 receptors (Toll-like receptor 4 and receptor for advanced glycation end products and pro-inflammatory cytokines (Tumor necrosis factor-alpha and interleukin-6) in both ischemic lobes, however, the up-regulation of these cytokines was more prominent in the ischemic lobes. In conclusion, selective warm I/R induces a substantial "sympathetic/bystander" effect on the non-ischemic lobes in terms of HMGB1 translocation and local cytokine production.
authors: Experimental Transplantation Surgery, Department of General, Visceral and Vascular Surgery, Friedrich-Schiller-University Jena, Drackendorfer straße1, 07747, Jena, Germany.
authors: Anding
authors: A
authors: Liu
authors: Olaf
authors: O
authors: Dirsch
authors: Haoshu
authors: H
authors: Fang
authors: Jian
authors: J
authors: Sun
authors: Hao
authors: H
authors: Jin
authors: Wei
authors: W
authors: Dong
authors: Uta
authors: U
authors: Dahmen
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D024243:HMGB1 Protein / Q000032:analysis / Q000378:metabolism*; D007150:Immunohistochemistry; D007511:Ischemia / Q000097:blood / Q000378:metabolism* / Q000473:pathology; D008099:Liver / Q000098:blood supply / Q000378:metabolism* / Q000473:pathology; D008297:Male; D016133:Polymerase Chain Reaction; D051381:Rats; D011917:Rats, Inbred Lew; D015427:Reperfusion Injury / Q000097:blood / Q000378:metabolism* / Q000473:pathology
chemical_list: D024243:HMGB1 Protein; C104200:Hbp1 protein, rat