title: G-quadruplex structures and G-quadruplex-interactive compounds.
abstract: G-quadruplexes are noncanonical secondary structures formed in DNA sequences containing consecutive runs of guanines. DNA G-quadruplexes have recently emerged as attractive cancer therapeutic targets. It has been shown that the 3' G-rich single-stranded overhangs of human telomeres can form G-quadruplex structures. G-quadruplex-interactive compounds have been shown to inhibit telomerase access as well as telomere capping. Nuclear magnetic resonance (NMR) spectroscopy has been shown to be a powerful method in determining the G-quadruplex structures under physiologically relevant conditions. We present the NMR methodology used in our research group for structure determination of G-quadruplexes in solution and their interactions with small molecule compounds. An example of a G-quadruplex structure formed in the human telomere sequence recently solved in our laboratory is used as an example.
authors: Department of Chemistry, BIO5 Institute, College of Pharmacy, The University of Arizona, Tucson, AZ, USA.
authors: Raveendra I
authors: RI
authors: Mathad
authors: Danzhou
authors: D
authors: Yang
mesh_terms: D054856:G-Quadruplexes; D006147:Guanine / Q000737:chemistry*; D006801:Humans; D006860:Hydrogen Bonding; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D011188:Potassium / Q000737:chemistry; D013329:Structure-Activity Relationship; D019098:Telomerase / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism; D016615:Telomere / Q000737:chemistry / Q000378:metabolism
chemical_list: D009942:Organometallic Compounds; D006147:Guanine; D019098:Telomerase; D011188:Potassium