title: A genome-scale shRNA resource for transgenic RNAi in Drosophila.
abstract: Existing transgenic RNAi resources in Drosophila melanogaster based on long double-stranded hairpin RNAs are powerful tools for functional studies, but they are ineffective in gene knockdown during oogenesis, an important model system for the study of many biological questions. We show that shRNAs, modeled on an endogenous microRNA, are extremely effective at silencing gene expression during oogenesis. We also describe our progress toward building a genome-wide shRNA resource.
authors: Department of Genetics, Harvard Medical School, Howard Hughes Medical Institute, Boston, Massachusetts, USA.
authors: Jian-Quan
authors: JQ
authors: Ni
authors: Rui
authors: R
authors: Zhou
authors: Benjamin
authors: B
authors: Czech
authors: Lu-Ping
authors: LP
authors: Liu
authors: Laura
authors: L
authors: Holderbaum
authors: Donghui
authors: D
authors: Yang-Zhou
authors: Hye-Seok
authors: HS
authors: Shim
authors: Rong
authors: R
authors: Tao
authors: Dominik
authors: D
authors: Handler
authors: Phillip
authors: P
authors: Karpowicz
authors: Richard
authors: R
authors: Binari
authors: Matthew
authors: M
authors: Booker
authors: Julius
authors: J
authors: Brennecke
authors: Lizabeth A
authors: LA
authors: Perkins
authors: Gregory J
authors: GJ
authors: Hannon
authors: Norbert
authors: N
authors: Perrimon
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics*; D005260:Female; D055785:Gene Knockdown Techniques; D005821:Genetic Techniques; D005822:Genetic Vectors; D049750:Genome, Insect; D035683:MicroRNAs / Q000235:genetics; D009866:Oogenesis / Q000235:genetics; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics
chemical_list: D017931:DNA Primers; D035683:MicroRNAs; D034741:RNA, Small Interfering