title: The Arabidopsis ESCRT protein-protein interaction network.
abstract: In yeast, endosomal sorting of monoubiquitylated transmembrane proteins is performed by a subset of the 19 "class E vacuolar protein sorting" proteins. The core machinery consists of 11 proteins that are organised in three complexes termed ESCRT I-III (endosomal sorting complex required for transport I-III) and is conserved in eukaryotic cells. While the pathway is well understood in yeast and animals, the plant ESCRT system is largely unexplored. At least one sequence homolog for each ESCRT component can be found in the Arabidopsis genome. Generally, sequence conservation between yeast/animals and the Arabidopsis proteins is low. To understand details about participating proteins and complex organization we have performed a systematic pairwise yeast two hybrid analysis of all Arabidopsis proteins showing homology to the ESCRT core machinery. Positive interactions were validated using bimolecular fluorescence complementation. In our experiments, most putative ESCRT components exhibited interactions with other ESCRT components that could be shown to occur on endosomes suggesting that despite their low homology to their yeast and animal counterparts they represent functional components of the plant ESCRT pathway.
authors: Botanical Institute, Lehrstuhl III, Biozentrum Köln, Zülpicher Strasse 47b, Cologne, Germany.
authors: Mojgan
authors: M
authors: Shahriari
authors: Klaus
authors: K
authors: Richter
authors: Channa
authors: C
authors: Keshavaiah
authors: Aneta
authors: A
authors: Sabovljevic
authors: Martin
authors: M
authors: Huelskamp
authors: Swen
authors: S
authors: Schellmann
mesh_terms: D000818:Animals; D017360:Arabidopsis / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D003198:Computer Simulation; D056827:Endosomal Sorting Complexes Required for Transport / Q000378:metabolism*; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D018613:Microscopy, Confocal; D008954:Models, Biological; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods*; D011523:Protoplasts / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics; D015398:Signal Transduction; D020798:Two-Hybrid System Techniques
chemical_list: D029681:Arabidopsis Proteins; D056827:Endosomal Sorting Complexes Required for Transport; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins