title: Direct-injection HPLC method of measuring micafungin in human plasma using a novel hydrophobic/hydrophilic hybrid ODS column.
abstract: A direct-injection HPLC-based method has been developed for determining amounts of micafungin in human plasma using a novel hydrophobic/hydrophilic hybrid ODS column. The method is easy to perform and requires only 10 μL of a filtered plasma sample. The chromatographic separations were carried out with a gradient mode. The fluorescence detection wavelengths of excitation and emission were set at 273 nm and 464 nm, respectively. Retention times for micafungin and IS were 22.4 and 23.7 min, respectively. Micafungin and FR195743 (IS) peaks were completely separated with little tailing, and no interference was observed. The calibration curve of micafungin showed good linearity in the range of 0.5-20.0 μg/mL (r(2)=1.00). The intra-day accuracy ranged from -4.5 to 5.3%. The inter-day accuracy ranged from -9.8 to 1.5%. The precisions were less than 10%. This method is useful for the determination of micafungin in human plasma.
authors: Laboratory of Drug Informatics, Gifu Pharmaceutical University, Gifu, Japan.
authors: Hiroaki
authors: H
authors: Uranishi
authors: Mitsuhiro
authors: M
authors: Nakamura
authors: Hiroki
authors: H
authors: Nakamura
authors: Yukari
authors: Y
authors: Ikeda
authors: Mayuko
authors: M
authors: Otsuka
authors: Zenichiro
authors: Z
authors: Kato
authors: Teruo
authors: T
authors: Tsuchiya
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods*; D054714:Echinocandins / Q000097:blood*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D016014:Linear Models; D055666:Lipopeptides / Q000097:blood*; D000077551:Micafungin; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D054714:Echinocandins; D055666:Lipopeptides; D000077551:Micafungin