title: Altered expression of genes in adipose tissues associated with reduced fat mass in patients with pancreatic cancer.
abstract: Loss of adipose tissue in patients with pancreatic cancer may involve altered gene expression. Peri-operative mRNA levels of 44 genes were analysed by RT-PCR in intra-abdominal (IAAT) and subcutaneous adipose tissue (SCAT) sampled from pancreatic ductal adenocarcinoma (PDAC) patients undergoing tumour resection (n = 20), and control patients without cancer (n = 11). Peri- and post-operative IAAT and SCAT masses were measured by computerized tomography. PDAC patients displayed 2.6- and 1.7-fold higher Zn-α2-glycoprotein (AZGP1) mRNA levels than controls in IAAT and SCAT, respectively (P < 0.01), but expression was not correlated with post-operative changes in fat masses. IAAT mass changes correlated with genes in lipid metabolism, inflammation and apoptosis: e.g. stearoyl-Coenzyme A desaturase 1 (SCD), tumour necrosis factor (TNF) and chemokine (C-C motif) ligand 2 (CCL2; MCP-1). Patients with PDAC displayed increased AZGP1 mRNA levels in both IAAT and SCAT, but expression of other genes may predict IAAT loss.
authors: Department of Nutrition, Institute of Basic Medical Sciences, University of Oslo, Norway. fred.haugen@medisin.uio.no
authors: F
authors: F
authors: Haugen
authors: K J
authors: KJ
authors: Labori
authors: H J
authors: HJ
authors: Noreng
authors: T
authors: T
authors: Buanes
authors: P O
authors: PO
authors: Iversen
authors: C A
authors: CA
authors: Drevon
mesh_terms: D054392:Adipokines; D000368:Aged; D000818:Animals; D017209:Apoptosis / Q000235:genetics; D002100:Cachexia / Q000378:metabolism; D021441:Carcinoma, Pancreatic Ductal / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D016022:Case-Control Studies; D018932:Chemokine CCL2 / Q000235:genetics / Q000378:metabolism; D005223:Fats / Q000737:chemistry / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D050152:Intra-Abdominal Fat / Q000737:chemistry / Q000378:metabolism; D050356:Lipid Metabolism / Q000235:genetics*; D008297:Male; D051379:Mice; D008875:Middle Aged; D010190:Pancreatic Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger; D013230:Stearoyl-CoA Desaturase / Q000235:genetics / Q000378:metabolism; D050507:Subcutaneous Fat, Abdominal / Q000737:chemistry / Q000378:metabolism; D014055:Tomography, Emission-Computed; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism; D000090203:Zn-Alpha-2-Glycoprotein
chemical_list: D054392:Adipokines; D002352:Carrier Proteins; D018932:Chemokine CCL2; D005223:Fats; D006023:Glycoproteins; D012333:RNA, Messenger; D013230:Stearoyl-CoA Desaturase; D014409:Tumor Necrosis Factor-alpha; C488891:AZGP1 protein, human; D000090203:Zn-Alpha-2-Glycoprotein; C523776:SCD1 protein, human