title: Enhanced antibacterial effect of silver nanoparticles obtained by electrochemical synthesis in poly(amide-hydroxyurethane) media.
abstract: In the present study, we report enhanced antimicrobial properties of 29 and 23 nm silver nanoparticles (Ag NPs) obtained by electrochemical synthesis in poly(amide-hydroxyurethane) media. Antibacterial activity assessed by disk diffusion method indicates that silver nanoparticles produced inhibition zones for both Escherichia coli and Staphylococcus aureus depending on silver concentration. The bacterial growth curve performed in the presence of silver nanoparticles showed a stronger antibacterial effect at lower concentrations than those described in the earlier reports. The effect was both dose and size dependent and was more pronounced against Gram negative bacteria than Gram positive one. The smallest Ag NPs used had a bactericidal effect resulting in killing E. coli cells. Scanning electron microscopy analysis indicated major damage and morphology changes of the silver nanoparticles treated bacterial cells. The major mechanism responsible for the antibacterial effect probably consists in clusters formation and nanoparticles anchorage to the bacterial cell surface.
authors: Faculty of Biology, Alexandru Ioan Cuza University, Iasi, Romania. stefanm@uaic.ro
authors: Marius
authors: M
authors: Stefan
authors: Stefan
authors: S
authors: Marius
authors: Lucian
authors: L
authors: Hritcu
authors: Hritcu
authors: H
authors: Lucian
authors: Marius
authors: M
authors: Mihasan
authors: Mihasan
authors: M
authors: Marius
authors: Daniela
authors: D
authors: Pricop
authors: Pricop
authors: P
authors: Daniela
authors: Irina
authors: I
authors: Gostin
authors: Gostin
authors: G
authors: Irina
authors: Romeo-Iulian
authors: RI
authors: Olariu
authors: Olariu
authors: O
authors: Romeo-Iulian
authors: Simona
authors: S
authors: Dunca
authors: Dunca
authors: D
authors: Simona
authors: Viorel
authors: V
authors: Melnig
authors: Melnig
authors: M
authors: Viorel
mesh_terms: D000577:Amides / Q000737:chemistry; D000900:Anti-Bacterial Agents / Q000737:chemistry*; D000890:Anti-Infective Agents / Q000494:pharmacology; D004148:Dioxolanes / Q000737:chemistry; D004563:Electrochemistry / Q000379:methods; D004926:Escherichia coli / Q000378:metabolism; D005374:Filtration; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D008855:Microscopy, Electron, Scanning / Q000379:methods; D036103:Nanotechnology / Q000379:methods; D009757:Nylons / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D012834:Silver / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000378:metabolism; D013696:Temperature; D014520:Urethane / Q000031:analogs & derivatives* / Q000737:chemistry
chemical_list: D000577:Amides; D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D004148:Dioxolanes; D009757:Nylons; D011108:Polymers; D014520:Urethane; D012834:Silver; C011286:N-hydroxyurethane; C031133:ethylene carbonate