title: Levels of lectin pathway proteins in plasma and synovial fluid of rheumatoid arthritis and osteoarthritis.
abstract: The innate immune system contributes to the development of rheumatoid arthritis (RA). A potent contributor to such processes is the complement system. The complement system is known to be activated in the inflammatory phases of osteoarthritis (OA). The lectin pathway of the complement system is activated through the recognition of pathogens or altered self-structures by mannan-binding lectin (MBL) or one of the three ficolins in collaboration with MBL-associated serine proteases (MASPs). We assessed the lectin pathway in plasma and synovial fluid (SF) of 27 RA patients and 30 OA patients by measuring MBL, MASP-2, MASP-3, M-ficolin, and H-ficolin. The concentration for all 5 proteins was significantly higher in plasma than in SF (P < 0.001) and the concentration in paired plasma and SF samples correlated in both RA and OA (significance levels between <0.001 and 0.02). The ratio of SF/plasma concentration was for all proteins significantly elevated in RA compared with OA patients (all P < 0.001). The M-ficolin concentration correlated with the neutrophils in both plasma (P = 0.01) and SF (P < 0.001) of RA, and in plasma of 78 controls (P = 0.03). To our knowledge, this is the first report on these proteins in SF, except for MBL where our results are in contrast to the one previous publication. The results support an important physiological role of the neutrophils in determining the M-ficolin levels in both RA and healthy adults. We suggest that quantifications of white blood cells should be included in future clinical investigations of M-ficolin.
authors: Department of Rheumatology, Aarhus University Hospital, Norrebrogade 44, 8000 Aarhus C, Denmark. c.ammitz@gmail.com
authors: C G
authors: CG
authors: Ammitzboll
authors: S
authors: S
authors: Thiel
authors: T
authors: T
authors: Ellingsen
authors: B
authors: B
authors: Deleuran
authors: Anette
authors: A
authors: Jorgensen
authors: J C
authors: JC
authors: Jensenius
authors: K
authors: K
authors: Stengaard-Pedersen
mesh_terms: D000328:Adult; D000368:Aged; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000276:immunology / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D003165:Complement System Proteins / Q000378:metabolism*; D003718:Denmark; D005260:Female; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D007113:Immunity, Innate; D037102:Lectins / Q000378:metabolism*; D008297:Male; D037601:Mannose-Binding Lectin / Q000378:metabolism; D050606:Mannose-Binding Protein-Associated Serine Proteases / Q000378:metabolism; D008875:Middle Aged; D009504:Neutrophils / Q000276:immunology / Q000378:metabolism; D010003:Osteoarthritis / Q000097:blood / Q000276:immunology / Q000378:metabolism*; D013582:Synovial Fluid / Q000276:immunology / Q000378:metabolism*; D000097604:Ficolins
chemical_list: D015415:Biomarkers; D003165:Complement System Proteins; D006023:Glycoproteins; D037102:Lectins; D037601:Mannose-Binding Lectin; D050606:Mannose-Binding Protein-Associated Serine Proteases; D000097604:Ficolins; C109662:FCN3 protein, human; C084067:MASP1 protein, human; C105296:MASP2 protein, human