title: Systematic errors in isothermal titration calorimetry: concentrations and baselines.
abstract: In the study of 1:1 binding by isothermal titration calorimetry, reagent concentration errors are fully absorbed in the data analysis, giving incorrect values for the key parameters--K, ΔH, and n--with no effect on the least-squares statistics. Reanalysis of results from an interlaboratory study of a selected biochemical process demonstrates that concentration errors are likely responsible for most of the overall statistical error in these parameters. The concentration errors are approximately 10%, greatly exceeding expected levels. Furthermore, examination of selected data sets reveals a surprising sensitivity to the baseline, suggesting a need for great care in treating dilution heats.
authors: Department of Chemistry, Vanderbilt University, Nashville, TN 37235, USA. joel.tellinghuisen@vanderbilt.edu
authors: Joel
authors: J
authors: Tellinghuisen
authors: John D
authors: JD
authors: Chodera
mesh_terms: D000818:Animals; D002151:Calorimetry / Q000379:methods*; D024402:Carbonic Anhydrase II / Q000737:chemistry; D002417:Cattle; D011485:Protein Binding; D013449:Sulfonamides / Q000737:chemistry; D013816:Thermodynamics; D000096926:Benzenesulfonamides
chemical_list: D024402:Carbonic Anhydrase II; D013449:Sulfonamides; D000096926:Benzenesulfonamides