title: TLR5-deficient mice lack basal inflammatory and metabolic defects but exhibit impaired CD4 T cell responses to a flagellated pathogen.
abstract: TLR5-deficient mice have been reported to develop spontaneous intestinal inflammation and metabolic abnormalities. However, we report that TLR5-deficient mice from two different animal colonies display no evidence of basal inflammatory disease, metabolic abnormalities, or enhanced resistance to Salmonella infection. In contrast, the absence of TLR5 hindered the initial activation and clonal expansion of intestinal flagellin-specific CD4 T cells following oral Salmonella infection. Together, these data demonstrate that a basal inflammatory phenotype is not a consistent feature of TLR5-deficient mice and document a novel role for TLR5 in the rapid targeting of flagellin by intestinal pathogen-specific CD4 T cells.
authors: Center for Infectious Diseases and Microbiology Translational Research, McGuire Translational Research Facility, University of Minnesota Medical School, Minneapolis, MN 55455, USA.
authors: Shirdi E
authors: SE
authors: Letran
authors: Seung-Joo
authors: SJ
authors: Lee
authors: Shaikh M
authors: SM
authors: Atif
authors: Adriana
authors: A
authors: Flores-Langarica
authors: Satoshi
authors: S
authors: Uematsu
authors: Shizuo
authors: S
authors: Akira
authors: Adam F
authors: AF
authors: Cunningham
authors: Stephen J
authors: SJ
authors: McSorley
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D005408:Flagellin / Q000276:immunology*; D007249:Inflammation / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D012481:Salmonella Infections, Animal / Q000276:immunology* / Q000378:metabolism* / Q000473:pathology; D051198:Toll-Like Receptor 5 / Q000172:deficiency* / Q000276:immunology
chemical_list: D051198:Toll-Like Receptor 5; D005408:Flagellin