title: A comparison of recombinant receptor-reporter gene bioassays and a total estrogen enzyme linked immunosorbent assay for the rapid screening of estrogenic activity in natural and waste waters.
abstract: In this study, we compared the performance as screening tools of two yeast-based recombinant receptor-reporter gene bioassays with a commercial ELISA kit for measurement of total estrogens. For WWTP effluents there was a very good correlation between the measured total estrogen concentrations (ELISA) and estrogenic activity by the hERα bioassay (r(2)=0.93), but not for the medERα bioassay (r(2)=0.50). For freshwater samples, the correlations between bioassay response and ELISA ES measurements were very good (r(2)>0.95). There was no correlation between bioassay response and ELISA ES measurements for estuarine samples.
authors: CAPIM, Bio 21 Institute, University of Melbourne, Parkville, VIC 3052, Australia.
authors: Mayumi
authors: M
authors: Allinson
authors: Fujio
authors: F
authors: Shiraishi
authors: Graeme
authors: G
authors: Allinson
mesh_terms: D001681:Biological Assay / Q000379:methods; D052244:Endocrine Disruptors / Q000633:toxicity*; D004784:Environmental Monitoring / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D004967:Estrogens / Q000633:toxicity*; D017930:Genes, Reporter; D011994:Recombinant Proteins; D018675:Toxicity Tests / Q000379:methods; D014865:Waste Disposal, Fluid; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D052244:Endocrine Disruptors; D004967:Estrogens; D011994:Recombinant Proteins; D014874:Water Pollutants, Chemical