title: [Effective agrobacterium-mediated transformation of chicory (Cichorium intybus L.) using vector with gene of tuberculosis antigene ESAT6].
abstract: The conditions of high efficient chicory transformation with Mycobacterium tuberculosis antigene ESAT6 have been determined. Transformation frequency was up to 86% when the cotyledons were cultivated within 3 days without cefotaxime and then 1 day without kanamycine. DNA PCR-analysis has shown the presence both of selective nptII and target esxA genes in all analysed plants. At the same time RT-PCR has shown the presence of nptII transcripts for eight analysed lines and esxA transcripts for only five analysed lines.
authors: N A
authors: NA
authors: Matveeva
authors: M Iu
authors: MIu
authors: Vasilenko
authors: A M
authors: AM
authors: Shakhovskiĭ
authors: M A
authors: MA
authors: Bannikova
authors: O Iu
authors: OIu
authors: Kvasko
authors: N V
authors: NV
authors: Kuchuk
mesh_terms: D000942:Antigens, Bacterial / Q000096:biosynthesis / Q000235:genetics*; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D018651:Cichorium intybus / Q000235:genetics* / Q000254:growth & development; D018744:DNA, Plant / Q000235:genetics; D005818:Genetic Engineering; D005822:Genetic Vectors; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000254:growth & development; D016133:Polymerase Chain Reaction; D014170:Transformation, Genetic; D032581:Tuberculosis Vaccines / Q000235:genetics
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D018744:DNA, Plant; C093276:ESAT-6 protein, Mycobacterium tuberculosis; D032581:Tuberculosis Vaccines