title: [Transgenic tomato plants expressing recA and NLS-recA-licBM3 genes as a model for studying meiotic recombination].
abstract: Homologous DNA recombination in eukaryotes is necessary to maintain genome stability and integrity and for correct chromosome segregation and formation of new haplotypes in meiosis. At the same time, genetic determination and nonrandomness of meiotic recombination restrict the introgression of genes and generation of unique genotypes. As one of the approaches to study and induce meiotic recombination in plants, it is recommended to use the recA gene of Escherichia coli. It is shown that the recA and NLS-recA-licBM3 genes have maternal inheritance and are expressed in the progeny of transgenic tomato plants. Plants expressing recA or NLS-recA-licBM3 and containing one T-DNA insertion do not differ in pollen fertility from original nontransgenic forms and can therefore be used for comparative studies of the effect of bacterial recombinases on meiotic recombination between linked genes.
authors: R A
authors: RA
authors: Komakhin
authors: V V
authors: VV
authors: Komakhina
authors: N A
authors: NA
authors: Miliukova
authors: I V
authors: IV
authors: Goldenkova-Pavlova
authors: O A
authors: OA
authors: Fadina
authors: A A
authors: AA
authors: Zhuchenko
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D048013:Acetivibrio thermocellus / Q000201:enzymology; D017930:Genes, Reporter; D006026:Glycoside Hydrolases / Q000096:biosynthesis* / Q000235:genetics; D018551:Solanum lycopersicum / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D008540:Meiosis; D019913:Nuclear Localization Signals; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D011938:Rec A Recombinases / Q000096:biosynthesis* / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000235:genetics; D011995:Recombination, Genetic
chemical_list: D001426:Bacterial Proteins; D019913:Nuclear Localization Signals; D011993:Recombinant Fusion Proteins; D011938:Rec A Recombinases; D006026:Glycoside Hydrolases; C028020:licheninase