title: Afp::mCherry, a red fluorescent transgenic reporter of the mouse visceral endoderm.
abstract: Live imaging of genetically encoded fluorescent protein reporters is increasingly being used to investigate details of the cellular behaviors that underlie the large-scale tissue rearrangements that shape the embryo. However, the majority of mouse fluorescent reporter strains are based on the green fluorescent protein (GFP). Mouse reporter strains expressing fluorescent colors other than GFP are therefore valuable for co-visualization studies with GFP, where relative positioning and relationship between two different tissues or compartments within cells are being investigated. Here, we report the generation and characterization of a transgenic Afp::mCherry mouse strain in which cis-regulatory elements from the Alpha-fetoprotein (Afp) locus were used to drive expression of the monomeric Cherry red fluorescent protein. The Afp::mCherry transgene is based on and recapitulates reporter expression of a previously described Afp::GFP strain. However, we note that perdurance of mCherry protein is not as prolonged as GFP, making the Afp::mCherry line a more faithful reporter of endogenous Afp expression. Afp::mCherry transgenic mice expressed mCherry specifically in the visceral endoderm and its derivatives, including the visceral yolk sac, gut endoderm, fetal liver, and pancreas of the embryo. The Afp::mCherry reporter was also noted to be expressed in other documented sites of Afp expression including hepatocytes as well as in pancreas, digestive tract, and brain of postnatal mice.
authors: Developmental Biology Program, Sloan-Kettering Institute, New York, New York, USA.
authors: Manuel
authors: M
authors: Viotti
authors: Sonja
authors: S
authors: Nowotschin
authors: Anna-Katerina
authors: AK
authors: Hadjantonakis
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004622:Embryo, Mammalian / Q000378:metabolism; D004707:Endoderm / Q000166:cytology* / Q000378:metabolism*; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics; D022781:Hepatocytes; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics*; D051379:Mice; D008822:Mice, Transgenic; D012045:Regulatory Sequences, Nucleic Acid; D019076:Transgenes; D014781:Viscera / Q000166:cytology / Q000378:metabolism; D015017:Yolk Sac / Q000196:embryology / Q000378:metabolism; D000509:alpha-Fetoproteins / Q000235:genetics*; D000097573:Red Fluorescent Protein
chemical_list: D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D000509:alpha-Fetoproteins; D000097573:Red Fluorescent Protein