title: Abnormal phospholipids distribution in the prefrontal cortex from a patient with schizophrenia revealed by matrix-assisted laser desorption/ionization imaging mass spectrometry.
abstract: Schizophrenia is one of the major psychiatric disorders, and lipids have focused on the important roles in this disorder. In fact, lipids related to various functions in the brain. Previous studies have indicated that phospholipids, particularly ones containing polyunsaturated fatty acyl residues, are deficient in postmortem brains from patients with schizophrenia. However, due to the difficulties in handling human postmortem brains, particularly the large size and complex structures of the human brain, there is little agreement regarding the qualitative and quantitative abnormalities of phospholipids in brains from patients with schizophrenia, particularly if corresponding brain regions are not used. In this study, to overcome these problems, we employed matrix-assisted laser desorption/ionization imaging mass spectrometry (IMS), enabling direct microregion analysis of phospholipids in the postmortem brain of a patient with schizophrenia via brain sections prepared on glass slides. With integration of traditional histochemical examination, we could analyze regions of interest in the brain at the micrometric level. We found abnormal phospholipid distributions within internal brain structures, namely, the frontal cortex and occipital cortex. IMS revealed abnormal distributions of phosphatidylcholine molecular species particularly in the cortical layer of frontal cortex region. In addition, the combined use of liquid chromatography/electrospray ionization tandem mass spectrometry strengthened the capability for identification of numerous lipid molecular species. Our results are expected to further elucidate various metabolic processes in the neural system.
authors: Department of Neuropsychiatry, School of Medicine, Fukushima Medical University, 1 Hikarigaoka, Fukushima, Fukushima, 960-1295, Japan.
authors: Junya
authors: J
authors: Matsumoto
authors: Yuki
authors: Y
authors: Sugiura
authors: Dai
authors: D
authors: Yuki
authors: Takahiro
authors: T
authors: Hayasaka
authors: Naoko
authors: N
authors: Goto-Inoue
authors: Nobuhiro
authors: N
authors: Zaima
authors: Yasuto
authors: Y
authors: Kunii
authors: Akira
authors: A
authors: Wada
authors: Qiaohui
authors: Q
authors: Yang
authors: Keisuke
authors: K
authors: Nishiura
authors: Hiroyasu
authors: H
authors: Akatsu
authors: Akira
authors: A
authors: Hori
authors: Yoshio
authors: Y
authors: Hashizume
authors: Takayuki
authors: T
authors: Yamamoto
authors: Keiko
authors: K
authors: Ikemoto
authors: Mitsutoshi
authors: M
authors: Setou
authors: Shin-ichi
authors: S
authors: Niwa
mesh_terms: D000368:Aged; D002853:Chromatography, Liquid; D006801:Humans; D008297:Male; D010743:Phospholipids / Q000378:metabolism*; D017397:Prefrontal Cortex / Q000378:metabolism*; D012559:Schizophrenia / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D010743:Phospholipids