title: Troglitazone induces apoptosis in gastric cancer cells through the NAG-1 pathway.
abstract: The non-steroidal anti-inflammatory drug-activated gene (NAG-1) is a newly identified member of the transforming growth factor (TGF)-β superfamily and plays significant roles in regulating proliferation and pro-apoptotic activities. In the present study, we studied the regulation of NAG-1 by troglitazone in the cultured gastric cancer cell line BGC-823. MTT and TUNEL assays demonstrated that troglitazone potentially inhibits the proliferation of the gastric cancer cell line and induces apoptosis in vitro in a dose- and time-dependent manner. Troglitazone induced concentration-dependent NAG-1 expression in the BGC-823 cells, as assessed using immunocytochemistry. Furthermore, troglitazone increased Egr-1 protein levels in a concentration-dependent manner. In conclusion, the present study suggests that troglitazone markedly impedes proliferation and pro-apoptotic activities in BGC-823 cells, and the mechanism may partly be through the Egr-1 pathway.
authors: Department of Gastroenterology, West China Hospital, Sichuan University, Chengdu 61004, PR China. wangch@scu.edu.cn
authors: Chunhui
authors: C
authors: Wang
authors: Jing
authors: J
authors: Wang
authors: Ping
authors: P
authors: Bai
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D002839:Chromans / Q000494:pharmacology*; D051766:Early Growth Response Protein 1 / Q000378:metabolism; D055436:Growth Differentiation Factor 15 / Q000378:metabolism*; D006801:Humans; D013274:Stomach Neoplasms / Q000188:drug therapy*; D045162:Thiazolidinediones / Q000494:pharmacology*; D000077288:Troglitazone
chemical_list: D000970:Antineoplastic Agents; D002839:Chromans; D051766:Early Growth Response Protein 1; C526140:GDF15 protein, human; D055436:Growth Differentiation Factor 15; D045162:Thiazolidinediones; D000077288:Troglitazone