title: Improving lipid production from bagasse hydrolysate with Trichosporon fermentans by response surface methodology.
abstract: Oleaginous yeast Trichosporon fermentans was proved to be able to use sulphuric acid-treated sugar cane bagasse hydrolysate as substrate to grow and accumulate lipid. Activated charcoal was shown as effective as the more expensive resin Amberlite XAD-4 for removing the inhibitors from the hydrolysate. To further improve the lipid production, response surface methodology (RSM) was used and a 3-level 4-factor Box-Behnken design was adopted to evaluate the effects of C/N ratio, inoculum concentration, initial pH and fermentation time on the cell growth and lipid accumulation of T. fermentans. Under the optimum conditions (C/N ratio 165, inoculum concentration 11%, initial pH 7.6 and fermentation time 9 days), a lipid concentration of 15.8g/L, which is quite close to the predicted value of 15.6g/L, could be achieved after cultivation of T. fermentans at 25°C on the pretreated bagasse hydrolysate and the corresponding lipid coefficient (lipid yield per mass of sugar, %) was 14.2. These represent a 32.8% improvement in the lipid concentration and a 21.4% increase in the lipid coefficient compared with the original values before optimization (11.9g/L and 11.7). This work further demonstrates that T. fermentans is a promising strain for lipid production and thus biodiesel preparation from abundant and inexpensive lignocellulosic materials.
authors: Laboratory of Applied Biocatalysis, College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China.
authors: Chao
authors: C
authors: Huang
authors: Hong
authors: H
authors: Wu
authors: Ri-feng
authors: RF
authors: Li
authors: Min-hua
authors: MH
authors: Zong
mesh_terms: D056804:Biofuels; D002482:Cellulose / Q000737:chemistry / Q000494:pharmacology*; D002606:Charcoal / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis / Q000187:drug effects; D008055:Lipids / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D011137:Polystyrenes / Q000737:chemistry; D011145:Polyvinyls / Q000737:chemistry; D014250:Trichosporon / Q000254:growth & development* / Q000378:metabolism
chemical_list: D056804:Biofuels; D008055:Lipids; D011137:Polystyrenes; D011145:Polyvinyls; D002606:Charcoal; C012101:XAD-4 resin; D002482:Cellulose; C027433:bagasse