title: In vivo visualization of RNA using the U1A-based tagged RNA system.
abstract: mRNA transport is a widely used method to achieve the asymmetric distribution of proteins within a cell or organism. In order to understand how RNA is transported, it is essential to utilize a system that can readily detect RNA movement in live cells. The tagged RNA system has recently emerged as a feasible non-invasive solution for such purpose. In this chapter, we describe in detail the U1A-based tagged RNA system. This system coexpresses U1Ap-GFP with the RNA of interest tagged with U1A aptamers, and has been proven to effectively track RNA in vivo. In addition, we provide further applications of the system for ribonucleoprotein complex purification by TAP-tagging the U1Ap-GFP construct.
authors: Department of Cell Biology, Yale University School of Medicine, New Haven, CT, USA.
authors: Sunglan
authors: S
authors: Chung
authors: Peter A
authors: PA
authors: Takizawa
mesh_terms: D018929:Cell Culture Techniques; D057054:Molecular Imaging / Q000379:methods*; D015335:Molecular Probes / Q000235:genetics / Q000378:metabolism*; D012313:RNA / Q000032:analysis* / Q000378:metabolism; D034443:RNA Transport; D012091:Repetitive Sequences, Nucleic Acid; D017412:Ribonucleoprotein, U1 Small Nuclear / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics; D014170:Transformation, Genetic
chemical_list: D015335:Molecular Probes; D017412:Ribonucleoprotein, U1 Small Nuclear; C075339:U1A protein; D012313:RNA