title: Synthesis of stable silica-dye hybrid nanomaterial as DNA carrier.
abstract: A new method is proposed for the fabrication of fluorescence-labeled and amine-modified silica nanoparticles for application as nonviral vectors in gene delivery. Highly monodisperse, stable fluorescent silica nanoparticles were prepared using 2,5-bis(5-tert-butyl-2-benzoxazolyl)thiophene and the water-in-oil microemulsion method. The green-fluorescent-protein gene can be easily combined onto the positively charged surfaces of nanoparticles to form a nanoparticle-DNA complex. The nanoparticle-DNA complex successfully passed through various barriers into the HeLa and HEK 293 K cells. The cytotoxicity of the PEI-coated and BBOT-encapsulated silica nanoparticles on both the HeLa and HEK 293T cell lines was found to be at an acceptable level for use as gene carriers when the particle concentration was below 125 microg/ml. The fluorescence intracellular images confirm the successful delivery of the nanoparticle-DNA complex and gene expression. The present work suggests the potential use of dye-incorporated silica nanoparticles in nonviral gene delivery.
authors: Department of Chemical and Biomolecular Engineering and Center for Ultramicrochemical Process Systems, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea
authors: Kyoung G
authors: KG
authors: Lee
authors: Jin Chul
authors: JC
authors: Kim
authors: Rinbok
authors: R
authors: Wi
authors: Jung Sun
authors: JS
authors: Min
authors: Jeong Keun
authors: JK
authors: Ahn
authors: Do Hyun
authors: DH
authors: Kim
mesh_terms: D004247:DNA / Q000008:administration & dosage* / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000235:genetics; D053758:Nanoparticles / Q000737:chemistry*; D010957:Plasmids / Q000235:genetics; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D012822:Silicon Dioxide; D004247:DNA