title: Induced pluripotent stem cell-derived cardiomyocytes as models for genetic cardiovascular disorders.
abstract: OBJECTIVE
  The development of induced pluripotent stem cell (iPSC) technology has led to many advances in the areas of directed cell differentiation and characterization. New methods for generating iPSC-derived cardiomyocytes provide an invaluable resource for the study of certain cardiovascular disorders. This review highlights the current technology in this field, its application thus far to the study of genetic disorders of the RAS/MAPK pathway and long-QT syndrome (LQTS), and future directions for the field.
  
  
  RESULTS
  Enhanced methods increase the efficiency of generating and stringently purifying iPSC-derived cardiomyocytes. The use of cardiomyocytes derived from patients with LEOPARD syndrome and LQTS has shed light on the molecular mechanisms of disease and validated their use as reliable human disease models.
  
  
  CONCLUSIONS
  The use of iPSC-derived cardiomyocytes to study genetic cardiovascular disorders will enable a deeper and more applicable understanding of the molecular mechanisms of human disease, as well as improving our ability to achieve successful cell-based therapies. Methods to efficiently generate these cells are improving and provide promise for future applications of this technology.
authors: Child Health and Development Institute, Mount Sinai School of Medicine, One Gustave Levy Place, New York, NY 10029, USA.
authors: Rebecca
authors: R
authors: Josowitz
authors: Xonia
authors: X
authors: Carvajal-Vergara
authors: Ihor R
authors: IR
authors: Lemischka
authors: Bruce D
authors: BD
authors: Gelb
mesh_terms: D002318:Cardiovascular Diseases / Q000235:genetics*; D002454:Cell Differentiation; D005347:Fibroblasts; D006801:Humans; D057026:Induced Pluripotent Stem Cells; D008133:Long QT Syndrome / Q000235:genetics; D008957:Models, Genetic; D032383:Myocytes, Cardiac