title: Investigation of irradiation by different nonablative lasers on primary cultured skin fibroblasts.
abstract: BACKGROUND
  A variety of lasers with different wavelengths and biological effects are widely used for nonablative skin rejuvenation, but the underlying mechanisms have not been fully investigated.
  
  
  OBJECTIVE
  To investigate the effects of irradiation by different nonablative lasers on collagen synthesis and the antioxidant status of cultured fibroblasts to identify a possible mechanism for laser photorejuvenation.
  
  
  METHODS
  Cultured skin fibroblasts were irradiated with three different lasers: 532 nm potassium-titanyl phosphate (KTP), 1064 nm Q-switched neodymium:yttrium-aluminium-garnet (Nd:Yag) and 1064 nm long-pulse Nd:YAG, and production of collagen and changes in lactate dehydrogenase (LDH), malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were assayed.
  
  
  RESULTS
  Irradiation by all three lasers led to a marked increase in collagen production. Two major antioxidant enzymes, SOD and GSH, were significantly increased, whereas MDA was markedly reduced after laser irradiation. No change in LDH activity was found between nonirradiated and irradiated fibroblasts.
  
  
  CONCLUSIONS
  This study indicates that the increased collagen synthesis by fibroblasts after laser treatment may be partly due to improved antioxidant capacity, which reduces oxidative stress and thus stimulates new collagen production.
authors: School of Life Science, East China Normal University, Shanghai, China.
authors: Y
authors: Y
authors: Weng
authors: Y
authors: Y
authors: Dang
authors: X
authors: X
authors: Ye
authors: N
authors: N
authors: Liu
authors: Z
authors: Z
authors: Zhang
authors: Q
authors: Q
authors: Ren
mesh_terms: D000535:Aluminum; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002478:Cells, Cultured / Q000528:radiation effects; D003094:Collagen / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism / Q000528:radiation effects*; D006909:Hydroxyproline / Q000378:metabolism; D045022:Lactate Dehydrogenases / Q000378:metabolism; D053844:Lasers, Solid-State; D008315:Malondialdehyde / Q000378:metabolism; D009354:Neodymium; D018384:Oxidative Stress / Q000528:radiation effects; D010710:Phosphates; D051381:Rats; D012060:Rejuvenation / Q000502:physiology; D014025:Titanium; D015019:Yttrium
chemical_list: D000975:Antioxidants; D010710:Phosphates; C503779:yttrium-aluminum-garnet; C064806:potassium titanylphosphate; D009354:Neodymium; D008315:Malondialdehyde; D015019:Yttrium; D003094:Collagen; D000535:Aluminum; D014025:Titanium; D045022:Lactate Dehydrogenases; D006909:Hydroxyproline