title: Structural basis of cooperative ligand binding by the glycine riboswitch.
abstract: The glycine riboswitch regulates gene expression through the cooperative recognition of its amino acid ligand by a tandem pair of aptamers. A 3.6 Å crystal structure of the tandem riboswitch from the glycine permease operon of Fusobacterium nucleatum reveals the glycine binding sites and an extensive network of interactions, largely mediated by asymmetric A-minor contacts, that serve to communicate ligand binding status between the aptamers. These interactions provide a structural basis for how the glycine riboswitch cooperatively regulates gene expression.
authors: Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520-8114, USA.
authors: Ethan B
authors: EB
authors: Butler
authors: Yong
authors: Y
authors: Xiong
authors: Jimin
authors: J
authors: Wang
authors: Scott A
authors: SA
authors: Strobel
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000378:metabolism; D001665:Binding Sites; D005786:Gene Expression Regulation; D005998:Glycine / Q000737:chemistry*; D008024:Ligands; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry; D058928:Riboswitch
chemical_list: D052157:Aptamers, Nucleotide; D008024:Ligands; D058928:Riboswitch; D012313:RNA; D005998:Glycine