title: Establishing diagnostic features for identifying the mucosa and submucosa of normal and cancerous gastric tissues by multiphoton microscopy.
abstract: BACKGROUND
  Establishing diagnostic features is essential and significant for developing multiphoton endoscopy to make an early diagnosis of gastric cancer at the cellular level. Until now, these diagnostic features have not been clearly described and understood.
  
  
  METHODS
  Study of diagnostic features based on multiphoton microscopy (MPM).
  
  
  OBJECTIVE
  Establishing diagnostic features to identify the mucosa and submucosa of human normal and cancerous gastric tissues by investigating their multiphoton microscopic images.
  
  
  METHODS
  Fujian Normal University and Fujian Provincial Tumor Hospital.
  
  
  METHODS
  Ten pairs of normal and cancerous specimens were obtained from 10 patients (ages 51-68 years) undergoing radical gastrectomy.
  
  
  METHODS
  MPM was performed on specimens.
  
  
  METHODS
  Establishment of diagnostic features.
  
  
  RESULTS
  MPM has the ability to exhibit not only the mucosal and submucosal microstructures of normal and cancerous gastric tissues but also the distribution and content of abnormal cells in these 2 layers. More importantly, it can provide the diagnostic features to qualitatively and quantitatively differentiate between normal and cancerous gastric tissues.
  
  
  CONCLUSIONS
  The selection bias and preparation of specimen.
  
  
  CONCLUSIONS
  These findings provide the groundwork for further establishing diagnostic criteria.
authors: Institute of Laser and Optoelectronics Technology, Fujian Normal University, Fuzhou, China. chenjianxin@fjnu.edu.cn
authors: Jianxin
authors: J
authors: Chen
authors: Shuangmu
authors: S
authors: Zhuo
authors: Gang
authors: G
authors: Chen
authors: Jun
authors: J
authors: Yan
authors: Hongquan
authors: H
authors: Yang
authors: Nenrong
authors: N
authors: Liu
authors: Liqin
authors: L
authors: Zheng
authors: Xingshan
authors: X
authors: Jiang
authors: Shusen
authors: S
authors: Xie
mesh_terms: D000368:Aged; D003937:Diagnosis, Differential; D005500:Follow-Up Studies; D005743:Gastrectomy; D005753:Gastric Mucosa / Q000648:ultrastructure*; D006801:Humans; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D008875:Middle Aged; D011184:Postoperative Period; D015203:Reproducibility of Results; D013274:Stomach Neoplasms / Q000473:pathology* / Q000601:surgery