title: LC-MS based quantification of 2'-ribosylated nucleosides Ar(p) and Gr(p) in tRNA.
abstract: RNA nucleosides are often naturally modified into complex non-canonical structures with key biological functions. Here we report LC-MS quantification of the Ar(p) and Gr(p) 2'-ribosylated nucleosides in tRNA using deuterium labelled standards, and the first detection of Gr(p) in complex fungi.
authors: Center for Integrated Protein Science (CiPSM) at the Department of Chemistry, LMU Munich, Butenandtstrasse 5-13, 81377 Munich, Germany.
authors: David
authors: D
authors: Pearson
authors: Antje
authors: A
authors: Hienzsch
authors: Mirko
authors: M
authors: Wagner
authors: Daniel
authors: D
authors: Globisch
authors: Veronika
authors: V
authors: Reiter
authors: Dilek
authors: D
authors: Özden
authors: Thomas
authors: T
authors: Carell
mesh_terms: D000249:Adenosine Monophosphate / Q000031:analogs & derivatives; D020029:Base Pairing; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D004926:Escherichia coli / Q000737:chemistry* / Q000378:metabolism; D006157:Guanosine Monophosphate / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D013058:Mass Spectrometry; D009705:Nucleosides / Q000032:analysis* / Q000737:chemistry*; D012331:RNA, Fungal / Q000737:chemistry* / Q000378:metabolism; D012343:RNA, Transfer / Q000737:chemistry* / Q000378:metabolism
chemical_list: D009705:Nucleosides; D012331:RNA, Fungal; C071213:O-beta-ribosyl(1''-2')-guanosine-5''-phosphate; C066273:O-beta-ribosyl(1''--2')adenosine-5''-phosphate; D000249:Adenosine Monophosphate; D006157:Guanosine Monophosphate; D012343:RNA, Transfer