title: Spectroscopic studies on TiO(2) enhanced binding of Hypocrellin B with DNA.
abstract: The binding of Hypocrellin B-TiO(2) chelate with DNA has been studied by using absorption, steady state fluorescence, cyclic voltammetry, time resolved fluorescence and laser flash photolysis measurements. The experimental results show that the presence of TiO(2) nanoparticles increases the binding of Hypocrellin B with DNA. The groove binding mode is confirmed by spectroscopic and docking studies. Laser flash photolysis studies confirm the presence of electrons in the conduction band of TiO(2) which will produce active oxygen species and results in damage of DNA indicating the potential application of Hypocrellin B-TiO(2) chelate in the field of photodynamic therapy (PDT).
authors: School of Chemistry, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India.
authors: Ganesan
authors: G
authors: Paramaguru
authors: Rajadurai Vijay
authors: RV
authors: Solomon
authors: Ponnambalam
authors: P
authors: Venuvanalingam
authors: Rajalingam
authors: R
authors: Renganathan
mesh_terms: D000818:Animals; D001665:Binding Sites / Q000187:drug effects; D002417:Cattle; D002614:Chelating Agents / Q000737:chemistry* / Q000494:pharmacology; D004247:DNA / Q000737:chemistry* / Q000187:drug effects; D004249:DNA Damage; D004563:Electrochemistry; D005453:Fluorescence; D007834:Lasers; D008958:Models, Molecular; D015394:Molecular Structure; D009994:Osmolar Concentration; D010569:Perylene / Q000031:analogs & derivatives* / Q000737:chemistry; D010782:Photolysis; D011809:Quinones / Q000737:chemistry*; D017382:Reactive Oxygen Species; D013050:Spectrometry, Fluorescence; D014025:Titanium / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D002614:Chelating Agents; D011809:Quinones; D017382:Reactive Oxygen Species; C009495:titanium dioxide; D010569:Perylene; D004247:DNA; C427203:calf thymus DNA; D014025:Titanium; C064522:hypocrellin B