title: Infection of human coronary artery endothelial cells by group B streptococcus contributes to dysregulation of apoptosis, hemostasis, and innate immune responses.
abstract: Early onset sepsis due to group B streptococcus leads to neonatal morbidity, increased mortality, and long-term neurological deficencies. Interaction between septicemic GBS and confluent monolayers of human coronary artery endothelial cells (HCAECs) was analyzed by genome wide expression profiling. In total, 124 genes were differentially expressed (89 upregulated, 35 downregulated) based on a more than 3-fold difference to control HCAEC. Regulated genes are involved in apoptosis, hemostasis, oxidative stress response, infection, and inflammation. Regulation of selected genes and proteins identified in the gene array analysis was confirmed by Real-time RT-PCR assay (granulocyte chemotactic protein 2), ELISA (urokinase, cyclooxygenase 2, granulocyte chemotactic protein 1), and western blotting (Heme oxygenase1, BCL2 interacting protein) at various time points between 4 and 24 hours. These results indicate that GBS infection might influence signalling pathways leading to impaired function of the innate immune system and hemorrhagic and inflammatory complications during GBS sepsis.
authors: Institute of Clinical Biochemistry and Pathobiochemistry, University of Wuerzburg, D-97080 Wuerzburg, Germany. beyrich_c@klinik.uni-wuerzburg.de
authors: Claudia
authors: C
authors: Beyrich
authors: Jürgen
authors: J
authors: Löffler
authors: Anna
authors: A
authors: Kobsar
authors: Christian P
authors: CP
authors: Speer
authors: Susanne
authors: S
authors: Kneitz
authors: Martin
authors: M
authors: Eigenthaler
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D002478:Cells, Cultured; D003331:Coronary Vessels / Q000166:cytology; D042783:Endothelial Cells / Q000166:cytology / Q000382:microbiology* / Q000502:physiology*; D020869:Gene Expression Profiling; D006487:Hemostasis / Q000502:physiology*; D006801:Humans; D007113:Immunity, Innate / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis; D018805:Sepsis / Q000382:microbiology / Q000503:physiopathology; D013290:Streptococcal Infections / Q000503:physiopathology*; D013292:Streptococcus agalactiae / Q000472:pathogenicity*