title: Proteome of the Caenorhabditis elegans oocyte.
abstract: Oocytes were purified from the temperature-sensitive fertilization-defective fer-1(b232ts) mutant of the nematode Caenorhabditis elegans and used for comprehensive mass spectrometric analysis. Using stringent criteria, 1165 C. elegans proteins were identified; at lower stringency, an additional 288 proteins were identified. We validate the high degree of sample purity and evaluate several possible sources of bias in the proteomic data. We compare the classes of proteins identified in the current oocyte proteome with protein classes identified in our previously determined oocyte transcriptome. The oocyte proteome appears enriched in proteins likely to be needed immediately upon fertilization, whereas the transcriptome appears enriched in molecules and processes needed later in embryogenesis. The current study provides fundamental background information for future more detailed studies of oocyte biology.
authors: Department of Biochemistry and Molecular Biology, Alberta Children's Hospital Research Institute of Child and Maternal Health, University of Calgary, Calgary, Alberta, Canada T2N 4N1.
authors: John K
authors: JK
authors: Chik
authors: David C
authors: DC
authors: Schriemer
authors: Sarah J
authors: SJ
authors: Childs
authors: James D
authors: JD
authors: McGhee
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000378:metabolism*; D005260:Female; D013058:Mass Spectrometry / Q000379:methods; D009865:Oocytes / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*
chemical_list: D020543:Proteome