title: Fluorescence enhancement effect for the determination of polychlorinated biphenyls with bovine serum albumin.
abstract: A sensitive and selective method for the trace determination of 3, 3', 4, 4'-tetrachlorobiphenyl (PCB77) by using bovine serum albumin (BSA) as a fluorescence probe was introduced. Under optimum conditions, the enhanced fluorescence intensity was proportional to the concentration of polychlorinated biphenyls in the range of 8.9 × 10(-8)-5.0 × 10(-6) mol L(-1) for PCB77, and 5.0 × 10(-7)-5.0 × 10(-6) mol L(-1) for 2, 2', 5, 5'-tetrachlorbiphenyl (PCB52). The detection limits (S/N = 3) of PCB77 and PCB52 were 2.6 × 10(-8) mol L(-1) and 2.9 × 10(-7) mol L(-1), respectively. Furthermore, the fluorescence enhancement mechanism was discussed in detail. Results indicated that fluorescence enhancement of the system originated from the formation of BSA-PCBs complexes. In addition, PCBs were mainly bound to the tyrosine residues in BSA molecules.
authors: Key Laboratory of Colloid and Interface Chemistry (Shandong University), Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, People's Republic of China.
authors: Fengju
authors: F
authors: Zhang
authors: Xia
authors: X
authors: Wu
authors: Jinhua
authors: J
authors: Zhan
mesh_terms: D000818:Animals; D002417:Cattle; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D011078:Polychlorinated Biphenyls / Q000032:analysis*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes; D012710:Serum Albumin, Bovine; D011078:Polychlorinated Biphenyls