title: Muscle fiber type-predominant promoter activity in lentiviral-mediated transgenic mouse.
abstract: Variations in gene promoter/enhancer activity in different muscle fiber types after gene transduction was noticed previously, but poorly analyzed. The murine stem cell virus (MSCV) promoter drives strong, stable gene expression in hematopoietic stem cells and several other cells, including cerebellar Purkinje cells, but it has not been studied in muscle. We injected a lentiviral vector carrying an MSCV-EGFP cassette (LvMSCV-EGFP) into tibialis anterior muscles and observed strong EGFP expression in muscle fibers, primary cultured myoblasts, and myotubes isolated from injected muscles. We also generated lentiviral-mediated transgenic mice carrying the MSCV-EGFP cassette and detected transgene expression in striated muscles. LvMSCV-EGFP transgenic mice showed fiber type-dependent variations in expression: highest in types I and IIA, intermediate in type IID/X, and lowest in type IIB fibers. The soleus and diaphragm muscles, consisting mainly of types I and IIA, are most severely affected in the mdx mouse model of muscular dystrophy. Further analysis of this promoter may have the potential to achieve certain gene expression in severely affected muscles of mdx mice. The Lv-mediated transgenic mouse may prove a useful tool for assessing the enhancer/promoter activities of a variety of different regulatory cassettes.
authors: Department of Neurology, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan.
authors: Tomohiro
authors: T
authors: Suga
authors: En
authors: E
authors: Kimura
authors: Yuka
authors: Y
authors: Morioka
authors: Masahito
authors: M
authors: Ikawa
authors: Sheng
authors: S
authors: Li
authors: Katsuhisa
authors: K
authors: Uchino
authors: Yuji
authors: Y
authors: Uchida
authors: Satoshi
authors: S
authors: Yamashita
authors: Yasushi
authors: Y
authors: Maeda
authors: Jeffrey S
authors: JS
authors: Chamberlain
authors: Makoto
authors: M
authors: Uchino
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D049452:Green Fluorescent Proteins / Q000235:genetics; D016086:Lentivirus / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009210:Myofibrils / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic
chemical_list: D017931:DNA Primers; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins