title: Embryonic stem cell factors undifferentiated transcription factor-1 (UFT-1) and reduced expression protein-1 (REX-1) are widely expressed in human skin and may be involved in cutaneous differentiation but not in stem cell fate determination.
abstract: Undifferentiated transcription factor-1 (UTF-1) and reduced expression protein-1 (REX-1) are used as markers for the undifferentiated state of pluripotent stem cells. Because no highly specific cytochemical marker for epidermal stem cells has yet been identified, we investigated the expression pattern of these markers in human epidermis and skin tumours by immunohistochemistry and in keratinocyte cell cultures. Both presumed stem cell markers were widely expressed in the epidermis and skin appendages. Distinct expression was found in the matrix cells of the hair shaft. Differentiation of human primary keratinocytes (KC) in vitro strongly downregulated UTF-1 and REX-1 expression. In addition, REX-1 was upregulated in squamous cell carcinomas, indicating a possible role of this transcription factor in malignant tumour formation. Our data point to a role for these proteins not only in maintaining KC stem cell populations, but also in proliferation and differentiation of matrix cells of the shaft and also suprabasal KC.
authors: Department of Dermatology, Medical University of Vienna, Vienna, Austria.
authors: Christina M
authors: CM
authors: Reinisch
authors: Michael
authors: M
authors: Mildner
authors: Peter
authors: P
authors: Petzelbauer
authors: Johannes
authors: J
authors: Pammer
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D002454:Cell Differentiation / Q000502:physiology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002478:Cells, Cultured; D015536:Down-Regulation / Q000502:physiology; D053595:Embryonic Stem Cells / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism* / Q000473:pathology; D051741:Kruppel-Like Transcription Factors / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism*; D039904:Pluripotent Stem Cells / Q000378:metabolism / Q000473:pathology; D012867:Skin / Q000378:metabolism* / Q000473:pathology; D012878:Skin Neoplasms / Q000378:metabolism* / Q000473:pathology; D015534:Trans-Activators / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*; D015854:Up-Regulation / Q000502:physiology
chemical_list: D015415:Biomarkers; D051741:Kruppel-Like Transcription Factors; D009687:Nuclear Proteins; D015534:Trans-Activators; D014157:Transcription Factors; C484738:UTF1 protein, human; C507976:ZFP42 protein, human