title: Aligned, isotropic and patterned carbon nanotube substrates that control the growth and alignment of Chinese hamster ovary cells.
abstract: Here we culture Chinese hamster ovary cells on isotropic, aligned and patterned substrates based on multiwall carbon nanotubes. The nanotubes provide the substrate with nanoscale topography. The cells adhere to and grow on all substrates, and on the aligned substrate, the cells align strongly with the axis of the bundles of the multiwall nanotubes. This control over cell alignment is required for tissue engineering; almost all tissues consist of oriented cells. The aligned substrates are made using straightforward physical chemistry techniques from forests of multiwall nanotubes; no lithography is required to make inexpensive large-scale substrates with highly aligned nanoscale grooves. Interestingly, although the cells strongly align with the nanoscale grooves, only a few also elongate along this axis: alignment of the cells does not require a pronounced change in morphology of the cell. We also pattern the nanotube bundles over length scales comparable to the cell size and show that the cells follow this pattern.
authors: Department of Physics, University of Surrey, Guildford, Surrey, UK.
authors: Che Azurahanim Che
authors: CA
authors: Abdullah
authors: Piyapong
authors: P
authors: Asanithi
authors: Eric W
authors: EW
authors: Brunner
authors: Izabela
authors: I
authors: Jurewicz
authors: Chiara
authors: C
authors: Bo
authors: Chihye Lewis
authors: CL
authors: Azad
authors: Raquel
authors: R
authors: Ovalle-Robles
authors: Shaoli
authors: S
authors: Fang
authors: Marcio D
authors: MD
authors: Lima
authors: Xavier
authors: X
authors: Lepro
authors: Steve
authors: S
authors: Collins
authors: Ray H
authors: RH
authors: Baughman
authors: Richard P
authors: RP
authors: Sear
authors: Alan B
authors: AB
authors: Dalton
mesh_terms: D000818:Animals; D016466:CHO Cells; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D018625:Microscopy, Atomic Force; D018613:Microscopy, Confocal; D036103:Nanotechnology / Q000379:methods*; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000633:toxicity / Q000648:ultrastructure; D013997:Time Factors
chemical_list: D037742:Nanotubes, Carbon