title: Highly efficient 3D fluorescence microscopy with a scanning laser optical tomograph.
abstract: Optical Projection Tomography (OPT) proved to be useful for the three-dimensional tracking of fluorescence signals in biological model organisms with sizes up to several millimeters. This tomographic technique detects absorption as well as fluorescence to create multimodal three-dimensional data. While the absorption of a specimen is detected very fast usually less than 0.1% of the fluorescence photons are collected. The low efficiency can result in radiation dose dependent artifacts such as photobleaching and phototoxicity. To minimize these effects as well as artifacts introduced due to the use of a CCD- or CMOS- camera-chip, we constructed a Scanning Laser Optical Tomograph (SLOT). Compared to conventional fluorescence OPT our first SLOT enhanced the photon collection efficiency a hundredfold.
authors: Biomedizinische Optik, Laser Zentrum Hannover e.V., Hollerithallee 8, D-30419 Hannover, Germany. R.Lorbeer@lzh.de
authors: Raoul-Amadeus
authors: RA
authors: Lorbeer
authors: Marko
authors: M
authors: Heidrich
authors: Christina
authors: C
authors: Lorbeer
authors: Diego Fernando
authors: DF
authors: Ramírez Ojeda
authors: Gerd
authors: G
authors: Bicker
authors: Heiko
authors: H
authors: Meyer
authors: Alexander
authors: A
authors: Heisterkamp
mesh_terms: D000818:Animals; D001921:Brain / Q000033:anatomy & histology; D004331:Drosophila melanogaster / Q000033:anatomy & histology; D005455:Fluorescent Antibody Technique; D007091:Image Processing, Computer-Assisted; D007814:Larva / Q000033:anatomy & histology; D007834:Lasers; D048188:Locusta migratoria / Q000033:anatomy & histology; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods*; D055096:Optical Devices; D014054:Tomography / Q000295:instrumentation*