title: Adipogenic differentiation of human mesenchymal stem cells.
abstract: Mesenchymal stem cells have the capability to differentiate into a number of cell types including adipocytes. The adipocytic phenotype is characterized by intracellular accumulation of lipid droplets as well as transcription of adipocyte-specific genes. This paper details a basic protocol for adipogenic induction of bone marrow and adipose tissue-derived stem cells, as well as protocols for staining lipid accumulation and the transcriptional analysis of PPAR-γ and aP2 by real-time RT-PCR.
authors: Laboratory for Stem Cell Research, Aalborg University, Aalborg, Denmark. trinef@hst.aau.dk
authors: Trine
authors: T
authors: Fink
authors: Vladimir
authors: V
authors: Zachar
mesh_terms: D050156:Adipogenesis; D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D001391:Azo Compounds / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology; D001896:Boron Compounds / Q000378:metabolism; D003584:Cytological Techniques / Q000379:methods*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013194:Staining and Labeling
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001391:Azo Compounds; D001896:Boron Compounds; C011049:oil red O