title: Comparison of mitomycin-c and heparin affects in experimental corrosive esophagitis on rats.
abstract: OBJECTIVE
  We comparative effects of mitomycin-c and heparin which have different mechanism of action in a minimal invasive corrosive esophagitis model which was formed by NaOH 40%.
  
  
  METHODS
  The study was performed on forty female Wistar albino rats; were divided into four equal groups each including ten animals. Group C (n=10); control, the group that esophagus was washed with normal saline, group I (n=10); injury group; alkali esophagus burn, not treated, group M (n=10); alkali esophagus burn, mitomycin-c treatment group, group H (n=10); alkali esophagus burn, heparin treatment group. The study was performed on a minimal invasive model which did not require general anesthesia and abdominal operation. In 28 day, all subjects were killed and their esophagus's were removed by thoraco-abdominal cut. Total esophagi from oropharynx to stomach were removed and they were examined macroscopically and microscopically and evaluated for esophageal tissue collagen deposition and histopathologic damage score.
  
  
  RESULTS
  When group C is compared with each of the other groups, statistically significant weight losses were detected; [(p<0.005, p<0.05, p<0.005), respectively]. Significant inflammation increase was detected in groups I, M and H in comparison to group C [(p<0.001, p<0, 0001, p<0.005)]. When granulation scores of groups were compared; statistically significant granulation increases were detected in groups I, M, and H [(p<0.05, p<0.05, p<0.05) compared to group C]. Significant collagen increase was detected in all 3 layers in groups; I, M and H according to group C [(p<0.05, p<0.05, p<0.05)]. Collagen increase in every 3 layers in groups M and H were significantly less according to group I [(p<0.05, p<0.05, p<0.05)]. Collagen increase in every 3 layers was less in group M than group H (p<0.05).
  
  
  CONCLUSIONS
  In corrosive esophagitis due to NaOH, heparin treatment is more effective in inflammation and granulation formation, mitomycin-c treatment is more effective in preventing the collagen accumulation step. Heparin decreases the tissue damage by preventing the inflammation and granulation formation; and prevents collagen accumulation and stricture development. As completing the effect of heparin; mitomycin prevents fibroblastic activity inhibition with direct collagen accumulation and stricture development strongly.
authors: Adnan Menderes University, Medicine faculty, General Thoracic Surgery, Aydin, Turkey. ekremsenturk@hotmail.com
authors: Ekrem
authors: E
authors: Senturk
authors: Engin
authors: E
authors: Pabuscu
authors: Serdar
authors: S
authors: Sen
authors: Cengiz
authors: C
authors: Unsal
mesh_terms: D000477:Alkylating Agents / Q000627:therapeutic use*; D000818:Animals; D002057:Burns, Chemical / Q000188:drug therapy / Q000209:etiology / Q000473:pathology; D002424:Caustics; D004195:Disease Models, Animal; D004941:Esophagitis / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D005260:Female; D005343:Fibrinolytic Agents / Q000627:therapeutic use*; D006493:Heparin / Q000627:therapeutic use*; D016685:Mitomycin / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D012972:Sodium Hydroxide
chemical_list: D000477:Alkylating Agents; D002424:Caustics; D005343:Fibrinolytic Agents; D016685:Mitomycin; D012972:Sodium Hydroxide; D006493:Heparin