title: A method to isolate and culture expand human dental pulp stem cells.
abstract: Dentinal repair in teeth occurs through the activity of specialized cells known as odontoblasts that are thought to be maintained by a precursor population associated with the perivascular cells within dental pulp tissue. We have previously isolated candidate dental pulp stem cells (DPSC) from adult human third molars, with the ability to generate clonogenic cell clusters (CFU-F: colony-forming units-fibroblastic), a high proliferation rate, and multi-potential differentiation in vitro. When cultured DPSC are transplanted into immunocompromised mice, they generated a dentin-like structure lined with human odontoblast-like cells that surrounded a pulp-like interstitial tissue, composed of collagen and a vascular network. The present protocol describes a methodology to generate highly purified preparations of human DPSC. This process involves the enzymatic digestion of fresh samples of human dental pulp tissue followed by the isolation of DPSC using magnetic bead cell separation, based on their expression of mesenchymal stem cell associated markers.
authors: Mesenchymal Stem Cell Group, Division of Haematology, Institute of Medical and Veterinary Science/Hanson Institute/CSCR, University of Adelaide, Adelaide, SA, Australia. stan.gronthos@health.sa.gov.au
authors: Stan
authors: S
authors: Gronthos
authors: Agnieszka
authors: A
authors: Arthur
authors: P Mark
authors: PM
authors: Bartold
authors: Songtao
authors: S
authors: Shi
mesh_terms: D017667:Adipocytes / Q000166:cytology; D000328:Adult; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods*; D003114:Colony-Forming Units Assay; D015925:Cryopreservation; D003782:Dental Pulp / Q000166:cytology*; D005434:Flow Cytometry; D006801:Humans; D008280:Magnetics; D051379:Mice; D008863:Microspheres; D009420:Nervous System / Q000166:cytology; D010006:Osteoblasts / Q000166:cytology; D013234:Stem Cells / Q000166:cytology*; D013535:Suspensions
chemical_list: D013535:Suspensions