title: dsRNA uptake in adult Drosophila.
abstract: RNA interference (RNAi) is a conserved sequence-specific gene silencing mechanism that is induced by double-stranded RNA (dsRNA). The development of methods that allow internalization of dsRNA and concomitant silencing of the desired gene has not stopped since the first demonstration of RNAi in Caenorhabditis elegans. In this chapter, we describe how to introduce exogenous dsRNA into adult Drosophila in order to interfere with endogenous or viral gene expression.
authors: CNRS URA 3015, Institut Pasteur, Viruses and RNAi group, Paris, France.
authors: Benjamin
authors: B
authors: Obadia
authors: Maria-Carla
authors: MC
authors: Saleh
mesh_terms: D000818:Animals; D001692:Biological Transport; D017931:DNA Primers / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D008845:Microinjections; D016133:Polymerase Chain Reaction; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D048348:Reverse Transcription
chemical_list: D017931:DNA Primers; D012330:RNA, Double-Stranded