title: Analysis of estrogen receptor alpha gene haplotype in Mexican mestizo patients with primary osteoarthritis of the knee.
abstract: The aim of this work was to test the association between estrogen receptor α gene (ERα) polymorphism and primary osteoarthritis (OA) of the knee in Mexican mestizo patients. A case-control study was conducted. Cases were patients >40 years of age, with a body mass index (BMI) ≤ 27 and radiologic score for OA of the knee of ≥2 according to Kellgren-Lawrence scale, and controls were subjects >40 years age with a radiologic score <2. Two restriction fragment length polymorphisms, PvuII (T/C; rs2234693), and XbaI (A/G; rs9340799), of the ERα were analyzed. Allelic haplotypes were constructed and non-conditional logistic regression was developed to evaluate risk magnitude through odds ratios (ORs) and 95% Confidence intervals (95% CI). Three different allelic haplotypes were identified: TA; CG, and CA. Unadjusted analysis of the haplotypes did not show significant associations; nonetheless, when data were adjusted for gender, age, and BMI, a significant decrease risk was observed for CG haplotype (OR [95% CI]) = 0.5 (0.3-0.9)] (P = 0.04). These results suggest that ERα gene CG haplotype could be associated with a reduced risk of primary knee OA in Mexican mestizo population.
authors: Departamento de Genética, Instituto Nacional de Rehabilitación (INR), Calzada México-Xochimilco No. 289, Col. Arenal de Guadalupe, Deleg. Tlalpan, 14889 México, DF, México.
authors: Verónica Marusa
authors: VM
authors: Borgonio-Cuadra
authors: Celia
authors: C
authors: González-Huerta
authors: Carolina
authors: C
authors: Duarte-Salazár
authors: María
authors: M
authors: de Los Ángeles Soria-Bastida
authors: Socorro
authors: S
authors: Cortés-González
authors: Antonio
authors: A
authors: Miranda-Duarte
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D047628:Estrogen Receptor alpha / Q000235:genetics*; D005006:Ethnicity / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006801:Humans; D007719:Knee Joint / Q000000981:diagnostic imaging; D016015:Logistic Models; D008297:Male; D008800:Mexico / Q000453:epidemiology; D008875:Middle Aged; D015999:Multivariate Analysis; D016017:Odds Ratio; D020370:Osteoarthritis, Knee / Q000000981:diagnostic imaging / Q000208:ethnology / Q000235:genetics*; D010641:Phenotype; D011110:Polymorphism, Genetic; D011859:Radiography; D018570:Risk Assessment; D012307:Risk Factors
chemical_list: C506487:ESR1 protein, human; D047628:Estrogen Receptor alpha