title: Modifications of commercial toxigenic Clostridium difficile PCR resulting in improved economy and workflow efficiency.
abstract: Expense inherent to molecular diagnostics may prevent laboratories from utilizing real-time PCR for Clostridium difficile infection. Frozen master mix and overnight aliquot modifications of the BD GeneOhm Cdiff assay failed to impact performance indices compared to the package insert protocol (P ≥ 0.31), provided accurate results, and decreased reagent expenditure.
authors: Wheaton Franciscan Laboratory, Wauwatosa, Wisconsin 53226, USA. Erik.Munson@wfhc.org
authors: Erik
authors: E
authors: Munson
authors: Dorothy
authors: D
authors: Bilbo
authors: Mary
authors: M
authors: Paul
authors: Maureen
authors: M
authors: Napierala
authors: Jeanne E
authors: JE
authors: Hryciuk
mesh_terms: D001427:Bacterial Toxins / Q000235:genetics*; D001431:Bacteriological Techniques / Q000191:economics* / Q000379:methods*; D016360:Clostridioides difficile / Q000235:genetics / Q000302:isolation & purification*; D003015:Clostridium Infections / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D016133:Polymerase Chain Reaction / Q000191:economics* / Q000379:methods*; D057188:Workflow
chemical_list: D001427:Bacterial Toxins