title: Cocaine increases ryanodine receptors via dopamine D1 receptors.
abstract: For review there are little available data on regulatory mechanisms of ryanodine receptor (RyR) expression with cocaine treatment, though methamphetamine was reported to up-regulate RyRs in mouse brain. This study attempted to investigate regulatory mechanisms of RyR expression using the cerebral cortical neurons in primary culture intermittently exposed to a psychostimulant, cocaine. Intermittent exposure to cocaine (10 μM) significantly enhanced RyR 1 and 2 proteins and their mRNA, but not RyR 3 expression in the neurons. These cocaine-induced increases of RyR proteins and their mRNA were dose-dependently blocked by a dopamine D1 receptor antagonist (SCH23390), but not by a dopamine D2 receptor antagonist (sulpiride). These results indicate a regulatory role of dopamine D1 receptors in RyR expression bycocaine.
authors: Departments of Pharmacology, Kawasaki Medical School, Matsushima 577, Kurashiki 701-0192, Japan.
authors: Kazuhiro
authors: K
authors: Kurokawa
authors: Koji
authors: K
authors: Mizuno
authors: Masahiro
authors: M
authors: Shibasaki
authors: Emi
authors: E
authors: Kiyokage
authors: Kazunori
authors: K
authors: Toida
authors: Seito
authors: S
authors: Ohkuma
mesh_terms: D000818:Animals; D021461:Animals, Outbred Strains; D001552:Benzazepines / Q000494:pharmacology; D002540:Cerebral Cortex / Q000166:cytology; D003042:Cocaine / Q000494:pharmacology*; D018492:Dopamine Antagonists / Q000494:pharmacology; D018765:Dopamine Uptake Inhibitors / Q000494:pharmacology; D051379:Mice; D009474:Neurons / Q000166:cytology / Q000187:drug effects*; D061251:Primary Cell Culture / Q000379:methods; D017447:Receptors, Dopamine D1 / Q000037:antagonists & inhibitors / Q000502:physiology*; D019837:Ryanodine Receptor Calcium Release Channel / Q000096:biosynthesis* / Q000235:genetics* / Q000378:metabolism; D013469:Sulpiride / Q000494:pharmacology; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology
chemical_list: D001552:Benzazepines; D018492:Dopamine Antagonists; D018765:Dopamine Uptake Inhibitors; D017447:Receptors, Dopamine D1; D019837:Ryanodine Receptor Calcium Release Channel; C534628:SCH 23390; D013469:Sulpiride; D003042:Cocaine