title: Telomere length analysis by quantitative fluorescent in situ hybridization (Q-FISH).
abstract: Length is a functional parameter of telomeres, the nucleoprotein structures that protect chromosome ends. The availability of highly specific, high-affinity probes for telomeric repeated sequences allowed the development of quantitative approaches aimed at measuring telomere length directly on chromosomes or in interphase nuclei. Here, we describe a general method for telomere quantitative FISH on metaphase chromosomes and discuss its most common applications in research.
authors: Telomeres & Cancer Laboratory, Institut Curie, Paris, France.
authors: Isabelle
authors: I
authors: Ourliac-Garnier
authors: Arturo
authors: A
authors: Londoño-Vallejo
mesh_terms: D002478:Cells, Cultured; D002875:Chromosomes / Q000737:chemistry; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D016615:Telomere / Q000737:chemistry* / Q000378:metabolism