title: Tracking down of laryngo-pharyngeal metastasis.
abstract: BACKGROUND
  In Romania, the most optimistic statistics give a 5 years survival rate in approximately 33% of laryngo-pharyngeal cancer patients. Considering that a cell carrying the viral DNA is originating from primary tumor, we have tested whether HPV DNA could be detected in the blood cell of patients with laryngeal cancer as a marker of disease progression and metastases.
  
  
  METHODS
  The study was performed on 85 patients (59 +/- 8.7 age) with laryngo-pharyngeal cancer. HPV DNA was detected in tumor using nested PCR with consensus primers, and also in local lymph nodes and/or blood cells from patients HPV positive in primary tumor.
  
  
  RESULTS
  HPV DNA was detected in 75.29% of analyzed tumours, and all HPV16 positive samples were confirmed by mRNA E6 expression. 56.3% of patients presented HPV DNA in peripheral circulation as confirmed by PCR with E6 HPV16 specific primers followed by Southern Blot.
  
  
  CONCLUSIONS
  Our results sustain that the detection of HPV DNA in blood is a "surrogate marker" of metastasis when extension of metastasis cannot be estimated, this observation is very important for management of cancer patients with laryngopharyngeal localization.
authors: Stefan S. Nicolau Institute of Virology, 285 Mihai Bravu, 030304, Bucharest, Romania. cbleotu@yahoo.com
authors: Coralia
authors: C
authors: Bleotu
authors: Cristian Radu
authors: CR
authors: Popescu
authors: Gabriela
authors: G
authors: Anton
authors: Adriana
authors: A
authors: Pleşa
authors: Raluca
authors: R
authors: Grigore
authors: Luminiţa
authors: L
authors: Welt
authors: Ioana
authors: I
authors: Aldea
authors: Roxana
authors: R
authors: Draguşel
authors: Mihaela
authors: M
authors: Chivu
authors: Georgiana
authors: G
authors: Matei
authors: Costin
authors: C
authors: Cernescu
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis; D002277:Carcinoma / Q000473:pathology / Q000821:virology; D002294:Carcinoma, Squamous Cell; D004273:DNA, Neoplasm / Q000737:chemistry / Q000235:genetics; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D006258:Head and Neck Neoplasms / Q000473:pathology / Q000821:virology; D006651:Histocytochemistry; D006801:Humans; D007822:Laryngeal Neoplasms / Q000473:pathology* / Q000821:virology*; D008875:Middle Aged; D018307:Neoplasms, Squamous Cell / Q000473:pathology / Q000821:virology; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000473:pathology* / Q000821:virology; D016133:Polymerase Chain Reaction; D000077195:Squamous Cell Carcinoma of Head and Neck
chemical_list: D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm; D004279:DNA, Viral