title: Hydroxylation of methylated CpG dinucleotides reverses stabilisation of DNA duplexes by cytosine 5-methylation.
abstract: Cytosine-5-methylation stabilises DNA duplexes and is associated with transcriptional repression; 5-methylcytosine undergoes hydroxylation to 5-hydroxymethylcytosine, a modification of unknown biological function. Spectroscopic and calorimetric analyses show that 5-hydroxymethylcytosine introduction reverses the stabilising effect of 5-methylcytosine, suggesting that in some contexts, 5-methylcytosine hydroxylation may, along with other factors, contribute to the alleviation of transcriptional repression.
authors: Department of Chemistry and the Oxford Centre for Integrative Systems Biology, Chemistry Research Laboratory, Oxford, UK.
authors: Armin
authors: A
authors: Thalhammer
authors: Anders S
authors: AS
authors: Hansen
authors: Afaf H
authors: AH
authors: El-Sagheer
authors: Tom
authors: T
authors: Brown
authors: Christopher J
authors: CJ
authors: Schofield
mesh_terms: D044503:5-Methylcytosine / Q000737:chemistry* / Q000031:analogs & derivatives; D001483:Base Sequence; D003596:Cytosine / Q000031:analogs & derivatives* / Q000737:chemistry; D003597:Cytosine Nucleotides / Q000737:chemistry*; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D019175:DNA Methylation; D015226:Dinucleoside Phosphates / Q000737:chemistry*; D006801:Humans; D006900:Hydroxylation; D008745:Methylation; D008969:Molecular Sequence Data; D015394:Molecular Structure; D016133:Polymerase Chain Reaction / Q000379:methods
chemical_list: D003597:Cytosine Nucleotides; D015226:Dinucleoside Phosphates; C011865:5-hydroxymethylcytosine; D044503:5-Methylcytosine; D003596:Cytosine; D004247:DNA