title: Enhanced expression of AtNHX1, in transgenic groundnut (Arachis hypogaea L.) improves salt and drought tolerence.
abstract: Salinity and drought are main threat to agriculture productivity, to avoid further losses it is necessary to improve the genetic material of crops against these stresses In this present study, AtNHX1, a vacuolar type Na(+)/H(+) antiporter gene driven by 35S promoter was introduced into groundnut using Agrobacterium tumefaciens transformation system. The stable integration of the AtNHX1 gene was confirmed by polymerase chain reaction (PCR) and southern blot analysis. It was found that transgenic plants having AtNHX1 gene are more resistant to high concentration of salt and water deprivation than the wild type plants. Salt and proline level in the leaves of the transgenic plants were also much higher than that of wild type plants. The results showed that overexpression of AtNHX1 gene not only improved salt tolerance but also drought tolerance in transgenic groundnut. Our results suggest that these plants could be cultivated in salt and drought-affected soils.
authors: National Institute for Genomics and Advanced Biotechnology, NARC, Islamabad, Pakistan.
authors: Muhammad Ahsan
authors: MA
authors: Asif
authors: Yusuf
authors: Y
authors: Zafar
authors: Javaid
authors: J
authors: Iqbal
authors: Muhammad Munir
authors: MM
authors: Iqbal
authors: Umer
authors: U
authors: Rashid
authors: Ghulam Muhammad
authors: GM
authors: Ali
authors: Anjuman
authors: A
authors: Arif
authors: Farhat
authors: F
authors: Nazir
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D010367:Arachis / Q000235:genetics*; D027682:Cation Transport Proteins / Q000378:metabolism*; D055864:Droughts; D018527:Hydroponics; D010641:Phenotype; D018515:Plant Leaves / Q000378:metabolism; D030821:Plants, Genetically Modified; D010957:Plasmids / Q000235:genetics; D011188:Potassium / Q000378:metabolism; D011392:Proline / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055049:Salt Tolerance / Q000235:genetics*; D012964:Sodium / Q000378:metabolism; D017923:Sodium-Hydrogen Exchangers / Q000378:metabolism*; D013312:Stress, Physiological / Q000235:genetics; D014170:Transformation, Genetic
chemical_list: D029681:Arabidopsis Proteins; D027682:Cation Transport Proteins; C117751:NHX1 protein, Arabidopsis; D017923:Sodium-Hydrogen Exchangers; D011392:Proline; D012964:Sodium; D011188:Potassium