title: Contribution of Nav1.1 to cellular synchronization and automaticity in spontaneous beating cultured neonatal rat ventricular cells.
abstract: Two factors of cell coupling influence cellular synchronization and automaticity: gap junction coupling and ion channels activity. However, the role of Na(+) channel isoforms underlying cell-to-cell interaction and cellular automaticity is not well understood. To address these questions, we studied mRNA expression of Na(+) channel isoforms and the effects of TTX on spontaneously beating cultured ventricle cells. Using RT-PCR technique we demonstrated the presence of Na(v)1.1 and Na(v)1.5 channels. The reduction of Na(v)1.1 channel activity disturbed cell-to-cell interaction and changed beating rates. Thus, Na(v)1.1 channel is involved in cellular synchronization and automaticity.
authors: Division of Physiome, Department of Physiology, Hyogo College of Medicine, Nishinomiya, Hyogo 663-8501, Japan. masumiya@hyo-med.ac.jp
authors: Haruko
authors: H
authors: Masumiya
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism; D002450:Cell Communication / Q000187:drug effects; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D004594:Electrophysiology / Q000379:methods; D006352:Heart Ventricles / Q000166:cytology / Q000378:metabolism; D009200:Myocardial Contraction / Q000502:physiology*; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000378:metabolism*; D062554:NAV1.5 Voltage-Gated Sodium Channel; D018408:Patch-Clamp Techniques / Q000379:methods; D020033:Protein Isoforms / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D026941:Sodium Channel Blockers / Q000494:pharmacology; D015222:Sodium Channels / Q000187:drug effects / Q000378:metabolism*; D013779:Tetrodotoxin / Q000494:pharmacology
chemical_list: D062554:NAV1.5 Voltage-Gated Sodium Channel; D020033:Protein Isoforms; C568322:Scn5a protein, rat; D026941:Sodium Channel Blockers; D015222:Sodium Channels; D013779:Tetrodotoxin