title: Mesenchymal-like stem cells derived from human parthenogenetic embryonic stem cells.
abstract: Human parthenogenetic embryonic stem cells (hpESCs) established from artificially activated oocytes have a wider immune-matching ability because of their homozygosity in the major histocompatibility complex alleles. Whether these cells possess the differentiation capacity similar to regular human embryonic stem cells (hESCs) derived from fertilized eggs is unclear. The aims of this study were to determine whether hpESCs could be differentiated into multipotent mesenchymal stem cell (MSC)-like cells in vitro and then compare these cells with those derived from hESCs. MSC-like cells were obtained from both hpESCs and hESCs, which exhibited similar cell surface marker expression profiles. Further analyses revealed that cells derived from hpESCs possessed stronger osteogenic but weaker adipogenic potentials compared with cells derived from hESCs. This is the first work that demonstrates the differentiation of hpESCs into multipotent MSC-like cells. These hpESCs could be a potential source for cell-based therapies.
authors: Shanghai Key Laboratory of Tissue Engineering, Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Jiao Tong University School of Medicine, National Tissue Engineering Center of China, Shanghai, China.
authors: Yao
authors: Y
authors: Chen
authors: Ai
authors: A
authors: Ai
authors: Zheng Ya
authors: ZY
authors: Tang
authors: Guang Dong
authors: GD
authors: Zhou
authors: Wei
authors: W
authors: Liu
authors: Yilin
authors: Y
authors: Cao
authors: Wen Jie
authors: WJ
authors: Zhang
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000943:Antigens, Differentiation / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D018920:Coculture Techniques; D024043:Collagen Type II / Q000378:metabolism; D058732:Embryoid Bodies; D053595:Embryonic Stem Cells / Q000378:metabolism / Q000502:physiology*; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D007381:Intermediate Filament Proteins / Q000378:metabolism; D050356:Lipid Metabolism; D059630:Mesenchymal Stem Cells / Q000378:metabolism / Q000502:physiology*; D009124:Muscle Proteins / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism; D064231:Nestin; D014746:Vimentin / Q000378:metabolism
chemical_list: D000943:Antigens, Differentiation; D024043:Collagen Type II; D007381:Intermediate Filament Proteins; D009124:Muscle Proteins; C577484:NES protein, human; D009419:Nerve Tissue Proteins; D064231:Nestin; D014746:Vimentin; D000469:Alkaline Phosphatase