title: The Pseudomonas aeruginosa quorum-sensing signal N-(3-oxododecanoyl) homoserine lactone can accelerate cutaneous wound healing through myofibroblast differentiation in rats.
abstract: Quorum sensing is a cell density-dependent gene regulation system in bacteria. N-(3-oxododecanoyl) homoserine lactone (3-oxo-C12-HSL) is used in the las quorum-sensing system in Pseudomonas aeruginosa, which is an opportunistic pathogen that causes many human diseases. Although many studies have investigated the sole effects of quorum sensing on several types of mammalian cells, including lung cells, little is known about the effects of quorum sensing on the cells associated with wound healing. To better understand the mechanism of bacterial wound infection, we investigated the effects of 3-oxo-C12-HSL on cells using a rat full-thickness wound-healing model. We found that the wound contraction was significantly increased at 24 h after the administration of 3-oxo-C12-HSL to the surface of granulation tissue. Differentiation of fibroblasts to myofibroblasts was induced in the in vivo wound-healing model and was confirmed in vitro using the rat fibroblastic cell line Rat-1. Cyclooxygenase (Cox)-2 expression was also induced in Rat-1 cells by 3-oxo-C12-HSL. This finding suggested that Cox-2 upregulation may be related to the inflammatory findings in the histological examinations, in which infiltrating polymorphonuclear neutrophils were observed at the wound site. Taken together, these results imply that mammals have a potential defense system against invading pathogens by responding to the presence of 3-oxo-C12-HSL and inducing the differentiation of fibroblasts to myofibroblasts as well as inflammation for accelerating wound healing.
authors: Department of Gerontological Nursing/Wound Care Management, Graduate School of Medicine, Faculty of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
authors: Gojiro
authors: G
authors: Nakagami
authors: Takeo
authors: T
authors: Minematsu
authors: Mayumi
authors: M
authors: Asada
authors: Takashi
authors: T
authors: Nagase
authors: Tomoko
authors: T
authors: Akase
authors: Lijuan
authors: L
authors: Huang
authors: Tomohiro
authors: T
authors: Morohoshi
authors: Tsukasa
authors: T
authors: Ikeda
authors: Yasunori
authors: Y
authors: Ohta
authors: Hiromi
authors: H
authors: Sanada
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D051546:Cyclooxygenase 2 / Q000096:biosynthesis; D004195:Disease Models, Animal; D015870:Gene Expression; D006651:Histocytochemistry; D006714:Homoserine / Q000031:analogs & derivatives* / Q000378:metabolism; D007249:Inflammation / Q000473:pathology; D008297:Male; D058628:Myofibroblasts / Q000187:drug effects*; D009504:Neutrophils / Q000276:immunology; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015854:Up-Regulation; D014945:Wound Healing / Q000187:drug effects*; D014947:Wounds and Injuries / Q000473:pathology
chemical_list: C109860:N-(3-oxododecanoyl)homoserine lactone; D006714:Homoserine; D051546:Cyclooxygenase 2; C489691:Ptgs2 protein, rat; D015107:4-Butyrolactone