title: [Affinity modification in a proteomic study of DNA repair ensembles].
abstract: The review concerns the use of the affinity modification method as an integral part of the modern proteomic analysis to search for and identification of proteins belonging to protein ensembles of DNA repair. Affinity modification is based on the preliminary formation of specific non-covalent complex between the target biopolymer and a reagent (chemically reactive analog of biopolymer or low molecular weight ligand) followed by formation of covalent bond between the reagent and the site of the target, to which the reagent is bound, that ensures the method specificity. This method is most widely and effectively used in the study of structural and functional aspects of protein-nucleic acids interactions. Upon construction of DNA probes, in addition to chemically reactive groups and structural elements involved in specific recognition of DNA by proteins, additional groups that facilitate the subsequent affinity isolation of DNA-protein cross-links, can be introduced into the reagent. The review covers recent examples affinity DNA-reactive probe in combination with mass spectrometric and immunological methods to search for and identification in cell extracts, proteins interacting with apurinic/apyrimidinic sites and the proteins recognizing the cross-links in DNA induced by cisplatin.
authors: S N
authors: SN
authors: Khodyreva
authors: O I
authors: OI
authors: Lavrik
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000378:metabolism; D001386:Azides / Q000737:chemistry / Q000528:radiation effects; D020137:Base Pair Mismatch; D002945:Cisplatin / Q000378:metabolism; D003432:Cross-Linking Reagents / Q000737:chemistry; D018736:DNA Adducts / Q000378:metabolism; D015342:DNA Probes / Q000737:chemistry* / Q000528:radiation effects; D004260:DNA Repair; D024243:HMGB1 Protein / Q000378:metabolism; D006801:Humans; D008190:Lyases / Q000378:metabolism; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D020543:Proteome / Q000032:analysis*; D012545:Schiff Bases / Q000737:chemistry; D014466:Ultraviolet Rays
chemical_list: D000970:Antineoplastic Agents; D001386:Azides; D003432:Cross-Linking Reagents; D018736:DNA Adducts; D015342:DNA Probes; D024243:HMGB1 Protein; D011506:Proteins; D020543:Proteome; D012545:Schiff Bases; C057270:cisplatin-DNA adduct; D008190:Lyases; D002945:Cisplatin