title: Genome-wide characterization of chromatin binding and nucleosome spacing activity of the nucleosome remodelling ATPase ISWI.
abstract: The evolutionarily conserved ATP-dependent nucleosome remodelling factor ISWI can space nucleosomes affecting a variety of nuclear processes. In Drosophila, loss of ISWI leads to global transcriptional defects and to dramatic alterations in higher-order chromatin structure, especially on the male X chromosome. In order to understand if chromatin condensation and gene expression defects, observed in ISWI mutants, are directly correlated with ISWI nucleosome spacing activity, we conducted a genome-wide survey of ISWI binding and nucleosome positioning in wild-type and ISWI mutant chromatin. Our analysis revealed that ISWI binds both genic and intergenic regions. Remarkably, we found that ISWI binds genes near their promoters causing specific alterations in nucleosome positioning at the level of the Transcription Start Site, providing an important insights in understanding ISWI role in higher eukaryote transcriptional regulation. Interestingly, differences in nucleosome spacing, between wild-type and ISWI mutant chromatin, tend to accumulate on the X chromosome for all ISWI-bound genes analysed. Our study shows how in higher eukaryotes the activity of the evolutionarily conserved nucleosome remodelling factor ISWI regulates gene expression and chromosome organization genome-wide.
authors: Dulbecco Telethon Institute c/o, Università degli Studi di Palermo, Palermo, Italy.
authors: Anna
authors: A
authors: Sala
authors: Maria
authors: M
authors: Toto
authors: Luca
authors: L
authors: Pinello
authors: Alessandra
authors: A
authors: Gabriele
authors: Valeria
authors: V
authors: Di Benedetto
authors: Antonia M R
authors: AM
authors: Ingrassia
authors: Giosuè
authors: G
authors: Lo Bosco
authors: Vito
authors: V
authors: Di Gesù
authors: Raffaele
authors: R
authors: Giancarlo
authors: Davide F V
authors: DF
authors: Corona
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D042002:Chromatin Assembly and Disassembly / Q000502:physiology*; D047369:Chromatin Immunoprecipitation; D003433:Crosses, Genetic; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004330:Drosophila; D029721:Drosophila Proteins / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology*; D023281:Genomics; D008297:Male; D009707:Nucleosomes / Q000502:physiology*; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D014157:Transcription Factors / Q000378:metabolism*; D014960:X Chromosome / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; C086468:ISWI protein; D009707:Nucleosomes; D014157:Transcription Factors; D000251:Adenosine Triphosphatases