title: In vitro antifungal susceptibilities of Candida isolates from patients with invasive candidiasis in Kuala Lumpur Hospital, Malaysia.
abstract: The in vitro antifungal susceptibilities of 159 clinical isolates of Candida species from patients with invasive candidiasis in Kuala Lumpur Hospital, Malaysia, were determined against amphotericin B, fluconazole, voriconazole, itraconazole and caspofungin. The most common species were Candida albicans (71 isolates), Candida parapsilosis (42 isolates), Candida tropicalis (27 isolates) and Candida glabrata (12 isolates). The susceptibility tests were carried out using an E-test. The MIC breakpoints were based on Clinical Laboratory Standards Institute criteria. Amphotericin B and voriconazole showed the best activities against all the isolates tested, with MIC(90) values of ≤1 µg ml(-1) for all major species. Only one Candida lusitaniae isolate was resistant to amphotericin B, and all the isolates were susceptible to voriconazole. In total, six isolates were resistant to fluconazole, comprising two isolates of C. albicans, two of C. parapsilosis, one C. tropicalis and one C. glabrata, and all of these isolates showed cross-resistance to itraconazole. The MIC(90) of itraconazole was highest for C. glabrata and C. parapsilosis. Caspofungin was active against most of the isolates except for five isolates of C. parapsilosis. The MIC(90) of caspofungin against C. parapsilosis was 3 µg ml(-1). In conclusion, amphotericin B remains the most active antifungal agent against most Candida species except for C. lusitaniae. Voriconazole is the best alternative for fluconazole- or itraconizole-resistant isolates. Although five of the C. parapsilosis isolates showed in vitro resistance to caspofungin, more clinical correlation studies need to be carried out to confirm the significance of these findings. Currently, despite the increase in usage of antifungals in our hospitals, especially in the management of febrile neutropenia patients, the antifungal-resistance problem among clinically important Candida isolates in Kuala Lumpur Hospital is not yet worrying. However, continued antifungal-susceptibility surveillance needs to be conducted to monitor the antifungal-susceptibility trends of Candida species and other opportunistic fungal pathogens.
authors: Bacteriology Unit, Institute for Medical Research, Kuala Lumpur, Malaysia.
authors: Fairuz
authors: F
authors: Amran
authors: Pathology Department, Kuala Lumpur Hospital, Kuala Lumpur, Malaysia.
authors: M Nazri
authors: MN
authors: Aziz
authors: Paediatric Department, Kuala Lumpur Hospital, Kuala Lumpur, Malaysia.
authors: Hishamshah M
authors: HM
authors: Ibrahim
authors: Bacteriology Unit, Institute for Medical Research, Kuala Lumpur, Malaysia.
authors: N H
authors: NH
authors: Atiqah
authors: Bacteriology Unit, Institute for Medical Research, Kuala Lumpur, Malaysia.
authors: S
authors: S
authors: Parameswari
authors: Bacteriology Unit, Institute for Medical Research, Kuala Lumpur, Malaysia.
authors: M R
authors: MR
authors: Hafiza
authors: Bacteriology Unit, Institute for Medical Research, Kuala Lumpur, Malaysia.
authors: M
authors: M
authors: Ifwat
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D002175:Candida / Q000145:classification / Q000187:drug effects* / Q000302:isolation & purification; D058365:Candidiasis, Invasive / Q000382:microbiology*; D025141:Drug Resistance, Fungal; D006761:Hospitals; D006801:Humans; D008296:Malaysia; D008826:Microbial Sensitivity Tests
chemical_list: D000935:Antifungal Agents