title: Psoriasis risk genes of the late cornified envelope-3 group are distinctly expressed compared with genes of other LCE groups.
abstract: Deletion of the late cornified envelope (LCE) genes LCE3B and LCE3C has recently been identified as a risk factor for psoriasis. Expression of 16 LCE genes of LCE groups 1, 2, 3, 5, and 6 was examined in vivo and in vitro. Quantitative PCR demonstrated that moderate to high LCE expression was largely confined to skin and a few oropharyngeal tissues. Genes of the LCE3 group demonstrated increased expression in lesional psoriatic epidermis and were induced after superficial injury of normal skin, whereas expression of members of other LCE groups was down-regulated under these conditions. Immunohistochemistry and immunoelectron microscopy demonstrated that LCE2 protein expression was restricted to the uppermost granular layer and the stratum corneum. Stimulation of in vitro reconstructed skin by several psoriasis-associated cytokines resulted in induction of LCE3 members. The data suggest that LCE proteins of groups 1, 2, 5, and 6 are involved in normal skin barrier function, whereas LCE3 genes encode proteins involved in barrier repair after injury or inflammation. These findings may provide clues to the mechanistic role of LCE3B/C deletion in psoriasis.
authors: Department of Dermatology, Radboud University Nijmegen Medical Centre, Nijmegen Centre for Molecular Life Sciences, Nijmegen, The Netherlands.
authors: Judith G M
authors: JG
authors: Bergboer
authors: Geuranne S
authors: GS
authors: Tjabringa
authors: Marijke
authors: M
authors: Kamsteeg
authors: Ivonne M J J
authors: IM
authors: van Vlijmen-Willems
authors: Diana
authors: D
authors: Rodijk-Olthuis
authors: Patrick A M
authors: PA
authors: Jansen
authors: Jean-Yves
authors: JY
authors: Thuret
authors: Masashi
authors: M
authors: Narita
authors: Akemi
authors: A
authors: Ishida-Yamamoto
authors: Patrick L J M
authors: PL
authors: Zeeuwen
authors: Joost
authors: J
authors: Schalkwijk
mesh_terms: D016022:Case-Control Studies; D055233:Cornified Envelope Proline-Rich Proteins / Q000096:biosynthesis* / Q000378:metabolism; D017353:Gene Deletion; D005786:Gene Expression Regulation; D005787:Gene Frequency; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D007249:Inflammation; D008856:Microscopy, Fluorescence / Q000379:methods; D016253:Microscopy, Immunoelectron / Q000379:methods; D011565:Psoriasis / Q000175:diagnosis* / Q000235:genetics* / Q000473:pathology; D012306:Risk; D012867:Skin / Q000378:metabolism
chemical_list: D055233:Cornified Envelope Proline-Rich Proteins; C549748:LCE3B protein, human; C524768:LCE3C protein, human