title: Effect of sub-acute exposure to acrylamide on GABAergic neurons and astrocytes in weaning rat cerebellum.
abstract: Occupational exposure and experimental intoxication of acrylamide (ACR) can produce skeletal muscle weakness and ataxia. In this study, we tested whether ACR would affect cerebellar function through the regulation of gamma-aminobutyric acid (GABA) and glial fibrillary acidic protein (GFAP) expression in cerebellum. Weaning male Sprague-Dawley rats were gavaged with ACR (5, 15, 30 mg/kg, 5 days per week) or saline for 4 weeks. Effects of ACR on the cerebellum were observed. For the 5 mg/kg group, no obvious change was observed, whereas moderate and severe ataxia were observed in the 15 mg/kg and 30 mg/kg groups, respectively. For the 15 mg/kg and 30 mg/kg groups, cerebellum concentrations of glutamate and GABA were dose-dependently decreased and increased, respectively. Moreover, the expression of GABA, the GABAergic presynaptic marker glutamate acid decarboxylase-65 (GAD65) and GFAP were significantly increased in those 2 groups. The results suggested that weaning rats were sensitive to ACR and that the toxic effects of ACR on the cerebellum may be associated with the increased expression of GABA and reactive astrocytes hypertrophy.
authors: Department of Anatomy, Histology and Embryology, Basic Medical College, Guangdong Pharmaceutical University, Guangzhou, China.
authors: Jing
authors: J
authors: Shi
authors: Yuxin
authors: Y
authors: Ma
authors: Meige
authors: M
authors: Zheng
authors: Zhigang
authors: Z
authors: Ruan
authors: Jing
authors: J
authors: Liu
authors: Sumin
authors: S
authors: Tian
authors: Dexing
authors: D
authors: Zhang
authors: Xinhong
authors: X
authors: He
authors: Guoying
authors: G
authors: Li
mesh_terms: D020106:Acrylamide / Q000633:toxicity*; D000704:Analysis of Variance; D000818:Animals; D001253:Astrocytes / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D001259:Ataxia / Q000139:chemically induced; D002531:Cerebellum / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D059330:GABAergic Neurons / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D005684:Gait / Q000187:drug effects; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D005968:Glutamate Decarboxylase / Q000378:metabolism; D018698:Glutamic Acid / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D059551:Toxicity Tests, Subacute; D014886:Weaning; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
chemical_list: D005904:Glial Fibrillary Acidic Protein; D020106:Acrylamide; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid; D005968:Glutamate Decarboxylase; C401141:glutamate decarboxylase 2