title: Effects ofcis-9,trans-11-conjugated linoleic acid on cancer cell cycle.
abstract: OBJECTIVE
  To determine the effect of cis-9, trans-11-conjugated linoleic acid on the cell cycle of mammary cancer cells (MCF-7) and its possible mechanism of inhibition cancer growth.
  
  
  METHODS
  Using cell culture and immunocytochemical techniques, we examined the cell growth, DNA synthesis, expression of PCNA, cyclin A, B(1), D(1), p16(ink4a) and p21(cip/wafl) of MCF-7 cells which were treated with various c9, t11-CLA concentrations (25 mM, 50 mM, 100 mM and 200 mM) of c9, t11-CLA for 24 and 48 h, with negative controls (0.1% ethanol).
  
  
  RESULTS
  The cell growth and DNA synthesis of MCF-7 cells were inhibited by c9, t11-CLA. MCF-7 cells, after treatment with various c9, t11-CLA doses mentioned above for 8 days, the inhibition frequency was 27.18%, 35.43%, 91.05%, and 92.86%, respectively and the inhibitory effect of c9, t11-CLA on DNA synthesis (except for 25 mM, 24 h) incorporated significantly less(3)H-TdR than did the negative control (P<0.05 andP<0.01). To further investigate the influence on the cell cycle progression, we found that c9, t11-CLA may arrest the cell cycle of MCF-7 cells. Immunocytochemical staining demonstrated that MCF-7 cells preincubated in media supplemented with different c9, t11-CLA concentrations at various times significantly decreased the expressions of PCNA, and Cyclin, A, B(1), D(1) compared with the negative controls (P<0.01), whereas the expressions of p16(ink4a) and p21(cip/wafl), cyclin-dependent kinases inhibitors (CDKI), were increased.
  
  
  CONCLUSIONS
  The cell growth and proliferation of MCF-7 cells is inhibited by c9, t11-CLA by blocking the cell cycle, which reduces expressions of cyclin A, B(1), D(1) and enhances expressions of CDKI (p16(ink4a) and p21(cip/wafl)).
authors: Public Health College of Harbin Medical University, 199 Dongdazhi Street, Nangang District, Harbin, Heilongijang, P.R. China, jiarenliu@yahoo.com.
authors: Jia Ren
authors: JR
authors: Liu
authors: Bing Qing
authors: BQ
authors: Chen
authors: Yan Mei
authors: YM
authors: Yang
authors: Xiao Hui
authors: XH
authors: Han
authors: Ying Ben
authors: YB
authors: Xue
authors: Xuan Ling
authors: XL
authors: Wang
authors: Yu Mei
authors: YM
authors: Zheng
authors: Rui Hai
authors: RH
authors: Liu