title: Identification of two spliced leader RNA transcripts from Perkinsus marinus.
abstract: Spliced leader (SL) variants are present in a number of mRNAs from Perkinsus marinus. Three different SLs of 22 nucleotides (nt) in length were previously reported, with a consensus sequence of (DCCGUAGCCAUYUUGGCUCAAG). A truncated 21 nt SL, with an (A) at nt-1 and a (U) deletion at nt-13, has also been reported. Here, we report an additional 21 nt SL variant with (G) at nt-1. Using cDNA analysis, a full-length SL RNA transcript was identified for both 21 nt SLs (SL2[A] and SL2[G]). This transcript is 81 nt in length and contains a conserved transcription termination sequence present in closely related dinoflagellates.
authors: Department of Natural Sciences, University of Maryland Eastern Shore, Princess Anne, Maryland 21853, USA.
authors: Jennifer L
authors: JL
authors: Hearne
authors: Joseph S
authors: JS
authors: Pitula
mesh_terms: D056893:Alveolata / Q000235:genetics*; D001483:Base Sequence; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D015870:Gene Expression; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D020038:RNA, Spliced Leader / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D018076:DNA, Complementary; D020038:RNA, Spliced Leader