title: Expanding the dynamic measurement range for polymeric nanoparticle pH sensors.
abstract: Conventional optical nanoparticle pH sensors that are designed for ratiometric measurements in cells have been based on utilizing one sensor fluorophore and one reference fluorophore in each nanoparticle, which results in a relatively narrow dynamic measurement range. This results in substantial challenges when conducting live cell measurements, which often leads to misleading results. In the present work we provide a simple solution to this problem.
authors: Technical University of Denmark, Department for Micro- and Nanotechnology, DTU Nanotech, 2800 Lyngby, Denmark.
authors: Honghao
authors: H
authors: Sun
authors: Kristoffer
authors: K
authors: Almdal
authors: Thomas L
authors: TL
authors: Andresen
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000295:instrumentation*; D058105:Polymerization; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D005456:Fluorescent Dyes