title: [Study on the correlation between polymorphisms of genes with susceptibility to tuberculosis and drug-resistant tuberculosis in Chinese Han population].
abstract: OBJECTIVE
  To investigate the distribution of polymorphisms of SLC11A1 gene, VDR gene, MBL gene and IFNG gene with susceptibility to tuberculosis (TB) in Chinese Han population suffering from drug-sensitive TB and drug-resistant TB so as to identify the correlation between gene polymorphisms and the development of drug-resistant TB.
  
  
  METHODS
  Single nucleotide polymorphisms (SNP) of VDR gene, SLC11A1 gene, MBL gene, IFNG gene were typed and analyzed by pyrosequencing, Real-time Probe and SNaPshot among 229 patients with drug-sensitive TB and 230 patients with drug-resistant TB.
  
  
  RESULTS
  The polymorphic foci of VDR gene from the drug-sensitive TB group and the drug-resistant TB group showed no significant difference (P > 0.05). The genotype of INT4 site and allelic frequency of SLC11A1 gene for drug-sensitive TB group were significantly different from those for drug-resistant TB group (P = 0.031, 0.046). If recessive inheritance was assumed, the genotypes of INT4 site from the two groups were significantly different (OR = 5.756, 95%CI: 1.261 - 26.269, P = 0.011). Considering the relationship between OR values under various combination, our findings confirmed that the genetic mode of INT4 site was in accordance with recessive inheritance. The genotypes of Q/P site and allelic frequencies of MBL gene from drug-sensitive and drug-resistant groups were significantly different (P = 0.029, 0.033). The difference still existed under the hypothesis of recessive inheritance (OR = 9.290, 95%CI: 1.167 - 73.949, P = 0.011). The polymorphic foci of IFNG gene from the two groups showed no significant difference.
  
  
  CONCLUSIONS
  INT4 sites on SLC11A1 gene and Q/P site on MBL gene were probably associated with the development of drug-resistant TB in Chinese Han population. Further study on this issue would be helpful in locating the population at high risk of drug-resistant TB and exploring the effective intervention to decrease the incidence of this disease.
authors: Tuberculosis Diagnosis and Treatment Center, Shanghai Pulmonary Hospital Affiliated to Tongji University, Tuberculosis Key Laboratory of Shanghai, China.
authors: Yi-dian
authors: YD
authors: Liu
authors: Rui-juan
authors: RJ
authors: Zheng
authors: He-ping
authors: HP
authors: Xiao
authors: Wei
authors: W
authors: Sha
authors: Qing
authors: Q
authors: Zhang
authors: Fu-rong
authors: FR
authors: Wu
authors: Hua
authors: H
authors: Sun
authors: Zhong-shun
authors: ZS
authors: Zhang
authors: Hai-yan
authors: HY
authors: Cui
authors: Zhi-bin
authors: ZB
authors: Liu
authors: Shen-jie
authors: SJ
authors: Tang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D044466:Asian People / Q000235:genetics; D027682:Cation Transport Proteins / Q000235:genetics*; D002681:China / Q000453:epidemiology; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D037601:Mannose-Binding Lectin / Q000235:genetics; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000187:drug effects; D011110:Polymorphism, Genetic; D018167:Receptors, Calcitriol / Q000235:genetics; D014376:Tuberculosis / Q000453:epidemiology / Q000235:genetics*; D018088:Tuberculosis, Multidrug-Resistant / Q000235:genetics*; D055815:Young Adult; D000099173:Natural Resistance-associated Macrophage Protein 1
chemical_list: D027682:Cation Transport Proteins; D037601:Mannose-Binding Lectin; D018167:Receptors, Calcitriol; D000099173:Natural Resistance-associated Macrophage Protein 1; C579457:VDR protein, human