title: [Effects of extracts from Patrinia heterophylla on gene expression patterns during morphogenesis of chicken limb buds in vivo].
abstract: OBJECTIVE
  To study the effects of the extracts from Patrinia heterophylla on gene expression patterns during morphogenesis of chicken limb buds in vivo.
  
  
  METHODS
  Implanted a bead into an chicken embryo, which was soaked in the extracts from Patrinia heterophylla. Detected the extracts-induced morphogenesis changes (Myf5, Myod and PCNA).
  
  
  RESULTS
  The extracts from Patrinia heterophylla (200 mg/mL) could affect limb bud development, reduce gene expression of MyfS, MyoD and PCNA.
  
  
  CONCLUSIONS
  The extracts from Patrinia heterophylla can inhibit cell differentiation and proliferation.
authors: Institute of Integrated Chinese and Western Medicine, Lanzhou University, Gansu 730000, China. huy@lzu.edu.cn
authors: Yan
authors: Y
authors: Hu
authors: Wei-Dong
authors: WD
authors: Cheng
authors: Wang-Hong
authors: WH
authors: Zhao
authors: Xin
authors: X
authors: Liu
authors: Xue-Xi
authors: XX
authors: Wang
mesh_terms: D020106:Acrylamide / Q000737:chemistry; D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002642:Chick Embryo; D002645:Chickens; D015536:Down-Regulation; D004337:Drug Carriers / Q000737:chemistry; D005121:Extremities / Q000196:embryology*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D018878:Limb Buds / Q000187:drug effects* / Q000196:embryology / Q000378:metabolism; D017570:MyoD Protein / Q000235:genetics / Q000378:metabolism; D051787:Myogenic Regulatory Factor 5 / Q000235:genetics / Q000378:metabolism; D032405:Patrinia / Q000737:chemistry*; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D017570:MyoD Protein; D051787:Myogenic Regulatory Factor 5; D010936:Plant Extracts; D018809:Proliferating Cell Nuclear Antigen; D020106:Acrylamide