title: Proteomic identification of an embryo-specific 1Cys-Prx promoter and analysis of its activity in transgenic rice.
abstract: Proteomic analysis of a rice callus led to the identification of 10 abscisic acid (ABA)-induced proteins as putative products of the embryo-specific promoter candidates. 5'-flanking sequence of 1 Cys-Prx, a highly-induced protein gene, was cloned and analyzed. The transcription initiation site of 1 Cys-Prx maps 96 nucleotides upstream of the translation initiation codon and a TATA-box and putative seed-specific cis-acting elements, RYE and ABRE, are located 26, 115 and 124 bp upstream of the transcription site, respectively. β-glucuronidase (GUS) expression driven by the 1 Cys-Prx promoters was strong in the embryo and aleurone layer and the activity reached up to 24.9 ± 3.3 and 40.5 ± 2.1 pmol (4 MU/min/μg protein) in transgenic rice seeds and calluses, respectively. The activity of the 1 Cys-Prx promoters is much higher than that of the previously-identified embryo-specific promoters, and comparable to that of strong endosperm-specific promoters in rice. GUS expression driven by the 1 Cys-Prx promoters has been increased by ABA treatment and rapidly induced by wounding in callus and at the leaf of the transgenic plants, respectively. Furthermore, ectopic expression of the GUS construct in Arabidopsis suggested that the 1 Cys-Prx promoter also has strong activity in seeds of dicot plants.
authors: Division of Applied Life Science (BK21 Program) and Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, 900 Gajwa-dong, Jinju 660-701, Republic of Korea.
authors: Je Hein
authors: JH
authors: Kim
authors: In Jung
authors: IJ
authors: Jung
authors: Dool Yi
authors: DY
authors: Kim
authors: Wahyu Indra
authors: WI
authors: Fanata
authors: Bo Hwa
authors: BH
authors: Son
authors: Jae Yong
authors: JY
authors: Yoo
authors: Rikno
authors: R
authors: Harmoko
authors: Ki Seong
authors: KS
authors: Ko
authors: Jeong Chan
authors: JC
authors: Moon
authors: Ho Hee
authors: HH
authors: Jang
authors: Woe Yeon
authors: WY
authors: Kim
authors: Jae-Yean
authors: JY
authors: Kim
authors: Chae Oh
authors: CO
authors: Lim
authors: Sang Yeol
authors: SY
authors: Lee
authors: Kyun Oh
authors: KO
authors: Lee
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism / Q000494:pharmacology; D000595:Amino Acid Sequence; D001483:Base Sequence; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D005966:Glucuronidase / Q000235:genetics; D008969:Molecular Sequence Data; D012275:Oryza / Q000187:drug effects / Q000235:genetics*; D010442:Peptide Chain Initiation, Translational; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000235:genetics*; D011401:Promoter Regions, Genetic; D040901:Proteomics; D012639:Seeds / Q000235:genetics*
chemical_list: D010940:Plant Proteins; D000040:Abscisic Acid; D005966:Glucuronidase