title: Immunological changes in canine peripheral blood leukocytes triggered by immunization with first or second generation vaccines against canine visceral leishmaniasis.
abstract: In this study, we summarized the major phenotypic/functional aspects of circulating leukocytes following canine immunization with Leishvaccine and Leishmune®. Our findings showed that Leishvaccine triggered early changes in the innate immunity (neutrophils and eosinophils) with late alterations on monocytes. Conversely, Leishmune(®) induced early phenotypic changes in both, neutrophils and monocytes. Moreover, Leishvaccine triggered mixed activation-related phenotypic changes on T-cells (CD4+ and CD8+ and B-lymphocytes, whereas Leishmune(®) promoted a selective response, mainly associated with CD8+ T-cell activation. Mixed cytokine profile (IFN-γ/IL-4) was observed in Leishvaccine immunized dogs whereas a selective pro-inflammatory pattern (IFN-γ/NO) was induced by Leishmune® vaccination. The distinct immunological profile triggered by Leishvaccine and Leishmune® may be a direct consequence of the distinct biochemical composition of these immunobiological, i.e. complex versus purified Leishmania antigen along with Bacillus Calmette-Guérin (BCG) versus saponin adjuvant. Both immunobiologicals are able to activate phagocytes and CD8+ T-cells and therefore could be considered as a putative vaccines against canine visceral leishmaniasis (CVL).
authors: Laboratório de Biomarcadores de Diagnóstico e Monitoração, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Av. Augusto de Lima, 1715 Barro Preto, Belo Horizonte, Minas Gerais, 30190-002, Brazil.
authors: Márcio Sobreira Silva
authors: MS
authors: Araújo
authors: Renata Aline
authors: RA
authors: de Andrade
authors: Renato
authors: R
authors: Sathler-Avelar
authors: Camila Paula
authors: CP
authors: Magalhães
authors: Andréa Teixeira
authors: AT
authors: Carvalho
authors: Mariléia Chaves
authors: MC
authors: Andrade
authors: Sabrina Sidney
authors: SS
authors: Campolina
authors: Maria Norma
authors: MN
authors: Mello
authors: Leonardo Rocha
authors: LR
authors: Vianna
authors: Wilson
authors: W
authors: Mayrink
authors: Alexandre Barbosa
authors: AB
authors: Reis
authors: Luiz Cosme Cotta
authors: LC
authors: Malaquias
authors: Luciana Morais
authors: LM
authors: Rocha
authors: Olindo Assis
authors: OA
authors: Martins-Filho
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000953:Antigens, Protozoan / Q000276:immunology; D016207:Cytokines / Q000276:immunology; D004283:Dog Diseases / Q000276:immunology / Q000469:parasitology* / Q000517:prevention & control; D004285:Dogs / Q000276:immunology / Q000469:parasitology; D005260:Female; D007113:Immunity, Innate / Q000276:immunology; D018314:Leishmania infantum / Q000276:immunology; D054332:Leishmaniasis Vaccines / Q000276:immunology / Q000494:pharmacology* / Q000627:therapeutic use; D007898:Leishmaniasis, Visceral / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D007962:Leukocytes / Q000276:immunology*; D008297:Male; D019001:Nitric Oxide Synthase / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; D016207:Cytokines; D054332:Leishmaniasis Vaccines; D019001:Nitric Oxide Synthase