title: Rescuing hepatocytes from iron-catalyzed oxidative stress using vitamins B1 and B6.
abstract: In the following rescue experiments, iron-mediated hepatocyte oxidative stress cytotoxicity was found to be prevented if vitamin B1 or B6 was added 1h after treatment with iron. The role of iron in catalyzing Fenton-mediated oxidative damage has been implicated in iron overload genetic diseases, carcinogenesis (colon cancer), Alzheimer's disease and complications associated with the metabolic syndrome through the generation of reactive oxygen species (ROS). The objectives of this study were to interpret the cytotoxic mechanisms and intracellular targets of oxidative stress using "accelerated cytotoxicity mechanism screening" techniques (ACMS) and to evaluate the rescue strategies of vitamins B1 and B6. Significant cytoprotection by antioxidants or ROS scavengers indicated that iron-mediated cytotoxicity could be attributed to reactive oxygen species. Of the B6 vitamers, pyridoxal was best at rescuing hepatocytes from iron-catalyzed lipid peroxidation (LPO), protein oxidation, and DNA damage, while pyridoxamine manifested greatest protection against ROS-mediated damage. Thiamin (B1) decreased LPO, mitochondrial and protein damage and DNA oxidation. Together, these results indicate that added B1 and B6 vitamins protect against the multiple targets of iron-catalyzed oxidative damage in hepatocytes. This study provides insight into the search for multi-targeted natural therapies to slow or retard the progression of diseases associated with Fenton-mediated oxidative damage.
authors: Department of Pharmaceutical Sciences, Faculty of Pharmacy, University of Toronto, Toronto, ON, Canada.
authors: Rhea
authors: R
authors: Mehta
authors: Liana
authors: L
authors: Dedina
authors: Peter J
authors: PJ
authors: O'Brien
mesh_terms: D000818:Animals; D000975:Antioxidants; D002083:Butylated Hydroxyanisole / Q000494:pharmacology; D002470:Cell Survival; D002478:Cells, Cultured; D020552:Comet Assay; D019610:Cytoprotection; D004249:DNA Damage / Q000187:drug effects; D003855:Deoxyribose / Q000032:analysis / Q000378:metabolism; D006146:Guanidines / Q000494:pharmacology; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects*; D007501:Iron / Q000633:toxicity*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008928:Mitochondria / Q000187:drug effects / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects*; D010618:Phenanthrolines / Q000032:analysis; D050050:Protein Carbonylation / Q000187:drug effects; D011733:Pyridoxamine / Q000494:pharmacology; D011794:Quercetin / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D013831:Thiamine / Q000494:pharmacology*; D025101:Vitamin B 6 / Q000494:pharmacology*; D014803:Vitamin B Complex / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D006146:Guanidines; D010618:Phenanthrolines; D017382:Reactive Oxygen Species; D014803:Vitamin B Complex; D002083:Butylated Hydroxyanisole; D003855:Deoxyribose; D011733:Pyridoxamine; D025101:Vitamin B 6; D011794:Quercetin; D007501:Iron; C004479:pimagedine; D013831:Thiamine