title: The 3' UTR variants in the GRP78 are not associated with overall survival in resectable hepatocellular carcinoma.
abstract: BACKGROUND
  Elevated glucose-regulated protein 78 (GRP78) levels in tissues have been known to be related with poor prognosis in hepatocellular carcinoma (HCC) patients. Though the variants in the 3' untranslated region (UTR) of GRP78 gene were not associated with HCC risk, we wonder whether these polymorphisms affect survival of HCC patients.
  
  
  RESULTS
  Blood samples of HCC patients were maintained in our specimen bank between 1996 to 2003. DNA from 576 unrelated and resectable patients with HCC was typed for rs16927997 (T>C), rs1140763 (T>C) and rs12009 (T>C) by TaqMan assays. The Kaplan-Meier method and log-rank test were used to estimate overall survival. Linkage disequilibrium (LD) analysis identified a total of 3 haplotypes and 6 diplotypes in this region. The distribution of haplotype was not related to the clinical characteristics. Univariate analysis showed that the allele, genotype, haplotype and diplotype did not effect the survival. None of the clinical features show a significant association (P(corrected)>0.05) with overall patient outcome in multiple comparisons.
  
  
  CONCLUSIONS
  There is no noteworthy influence of 3' UTR variants in the GRP78 on prognosis of resectable HCC in the Chinese population.
authors: Cancer Institute and Affiliated Tumor Hospital, Guangzhou Medical College, Guangzhou, China. bioxzhu@yahoo.com
authors: Xiao
authors: X
authors: Zhu
authors: Fang
authors: F
authors: Wang
authors: Marie C M
authors: MC
authors: Lin
authors: Linwei
authors: L
authors: Tian
authors: Wenguo
authors: W
authors: Fan
authors: Samuel S
authors: SS
authors: Ng
authors: Minjuan
authors: M
authors: Liu
authors: Jianqing
authors: J
authors: Huang
authors: Zhenhua
authors: Z
authors: Xu
authors: Dongpei
authors: D
authors: Li
authors: Hsiangfu
authors: H
authors: Kung
mesh_terms: D020413:3' Untranslated Regions; D000328:Adult; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D000091342:Endoplasmic Reticulum Chaperone BiP; D005260:Female; D005500:Follow-Up Studies; D006360:Heat-Shock Proteins / Q000235:genetics*; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D016019:Survival Analysis
chemical_list: D020413:3' Untranslated Regions; D000091342:Endoplasmic Reticulum Chaperone BiP; C000717247:HSPA5 protein, human; D006360:Heat-Shock Proteins