title: Strain improvement for enhanced production of cellulase in Trichoderma viride.
abstract: The filamentous fungi Trichoderma species produce extracellular cellulase. The current study was carried out to obtain an industrial strain with hyperproduction of cellulase. The wild-type strain, Trichoderma viride TL-124, was subjected to successive mutagenic treatments with UV irradiation, low-energy ion beam implantation, atmospheric pressure non-equilibrium discharge plasma (APNEDP), and N-methyl-N'-nitro-N-nitrosoguanidine to generate about 3000 mutants. Among these mutants, T. viride N879 strain exhibited the greatest relevant activity: 2.38-fold filter paper activity and 2.61-fold carboxymethyl cellulase, 2.18-fold beta-glucosidase, and 2.27-fold cellobiohydrolase activities, compared with the respective wild-type activities, under solid-state fermentation using the inexpensive raw material wheat straw as a substrate. This work represents the first application of APNEDP in eukaryotic microorganisms.
authors: State Key Laboratory for Agrobiotechnology, China Agricultural University Beijing 100193, China.
authors: F
authors: F
authors: Xu
authors: J
authors: J
authors: Wang
authors: S
authors: S
authors: Chen
authors: W
authors: W
authors: Qin
authors: Z
authors: Z
authors: Yu
authors: H
authors: H
authors: Zhao
authors: X
authors: X
authors: Xing
authors: H
authors: H
authors: Li
mesh_terms: D002480:Cellulase / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D002482:Cellulose / Q000378:metabolism*; D043366:Cellulose 1,4-beta-Cellobiosidase / Q000096:biosynthesis; D005285:Fermentation; D005374:Filtration; D015870:Gene Expression; D005800:Genes, Fungal / Q000187:drug effects / Q000528:radiation effects; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D016296:Mutagenesis / Q000187:drug effects / Q000528:radiation effects; D009154:Mutation / Q000187:drug effects / Q000528:radiation effects; D058626:Plasma Gases; D014242:Trichoderma / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism / Q000528:radiation effects; D014908:Triticum / Q000378:metabolism; D014466:Ultraviolet Rays; D001617:beta-Glucosidase / Q000096:biosynthesis
chemical_list: D058626:Plasma Gases; D008769:Methylnitronitrosoguanidine; D002482:Cellulose; D001617:beta-Glucosidase; D002480:Cellulase; C019826:carboxymethylcellulase; D043366:Cellulose 1,4-beta-Cellobiosidase