title: Zn(II)-cyclam based chromogenic sensors for recognition of ATP in aqueous solution under physiological conditions and their application as viable staining agents for microorganism.
abstract: Two chromogenic complexes, L.Zn (where L is (E)-4-((4-(1,4,8,11-tetraazacyclotetradecan-1-ylsulfonyl)phenyl)diazenyl)-N,N-dimethylaniline) and its [2]pseudorotaxane form (α-CD.L.Zn), were found to bind preferentially to adenosine triphosphate (ATP), among all other common anions and biologically important phosphate (AMP, ADP, pyrophosphate, and phosphate) ions in aqueous HEPES buffer medium of pH 7.2. Studies with live cell cultures of prokaryotic microbes revealed that binding of these two reagents to intercellular ATP, produced in situ, could be used in delineating the gram-positive and the gram-negative bacteria. More importantly, these dyes were found to be nontoxic to living microbes (eukaryotes and prokaryotes) and could be used for studying the cell growth dynamics. Binding to these two viable staining agents to intercellular ATP was also confirmed by spectroscopic studies on cell growth in the presence of different respiratory inhibitors that influence the intercellular ATP generation.
authors: Central Salt & Marine Chemicals Research Institute (CSIR), Bhavnagar, 364002 Gujarat, India.
authors: Prasenjit
authors: P
authors: Mahato
authors: Amrita
authors: A
authors: Ghosh
authors: Sanjiv K
authors: SK
authors: Mishra
authors: Anupama
authors: A
authors: Shrivastav
authors: Sandhya
authors: S
authors: Mishra
authors: Amitava
authors: A
authors: Das
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D001419:Bacteria / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D002470:Cell Survival; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation*; D004396:Coloring Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D006571:Heterocyclic Compounds / Q000737:chemistry*; D008853:Microscopy; D009942:Organometallic Compounds / Q000737:chemistry* / Q000378:metabolism; D012996:Solutions; D013194:Staining and Labeling; D014867:Water / Q000737:chemistry*; D015032:Zinc / Q000737:chemistry*
chemical_list: D004396:Coloring Agents; D006571:Heterocyclic Compounds; D009942:Organometallic Compounds; D012996:Solutions; D014867:Water; C024560:cyclam; D000255:Adenosine Triphosphate; D015032:Zinc