title: A comparative study of fluorescence-labelled enzyme activity methods for assaying phosphatase activity in phytoplankton. A possible bias in the enzymatic pathway estimations.
abstract: We compared different fluorescence-labelled enzyme activity (FLEA) methods for assaying phosphatase activity in phytoplankton. Unfixed and liquid incubations are devised. We demonstrated that the presence of intracellular labelling was persistent, which could point out a source of bias in ectoenzymatic activities measurements based either on the FLEA or classical methods.
authors: Unitat de Limnologia, Departament d'Ecologia i Centre de Recerca d'Alta Muntanya, CEAB-CSIC-Universitat de Barcelona, 645 Diagonal Av. 08028, Barcelona, Catalonia, Spain. diazdequijano@ub.edu
authors: Daniel
authors: D
authors: Díaz-de-Quijano
authors: Marisol
authors: M
authors: Felip
mesh_terms: D057075:Enzyme Assays / Q000379:methods*; D005453:Fluorescence; D010744:Phosphoric Monoester Hydrolases / Q000032:analysis* / Q000378:metabolism; D010839:Phytoplankton / Q000737:chemistry / Q000201:enzymology*
chemical_list: D010744:Phosphoric Monoester Hydrolases