title: Glycomics meets lipidomics--associations of N-glycans with classical lipids, glycerophospholipids, and sphingolipids in three European populations.
abstract: Recently, high-throughput technologies have been made available which allow the measurement of a broad spectrum of glycomics and lipidomics parameters in many samples. The aim of this study was to apply these methods and investigate associations between 46 glycan and 183 lipid traits measured in blood of 2041 Europeans from three different local populations (Croatia - VIS cohort; Sweden - NSPHS cohort; Great Britain - ORCADES cohort). N-glycans have been analyzed with High Performance Liquid Chromatography (HPLC) and lipids with Electrospray Ionization Tandem Mass Spectrometry (ESI-MS/MS) covering sterol lipids, glycerolipids, glycerophospholipids and sphingolipids in eight subclasses. Overall, 8418 associations were calculated using linear mixed effect models adjusted for pedigree, sex, age and multiple testing. We found 330 significant correlations in VIS. Pearson's correlation coefficient r ranged from -0.27 to 0.34 with corresponding p-values between 1.45 × 10(-19) and 4.83 × 10(-6), indicating statistical significance. A total of 71 correlations in VIS could be replicated in NSPHS (r = [-0.19; 0.35], p = [4.16 × 10(-18); 9.38 × 10(-5)]) and 31 correlations in VIS were also found in ORCADES (r = [-0.20; 0.24], p = [2.69 × 10(-10); 7.55 × 10(-5)]). However, in total only 10 correlations between a subset of triantennary glycans and unsaturated phosphatidylcholine, saturated ceramide, and sphingomyelin lipids in VIS (r = [0.18; 0.34], p = [2.98 × 10(-21); 1.69 × 10(-06)]) could be replicated in both NSPHS and ORCADES. In summary, the results show strong and consistent associations between certain glycans and lipids in all populations, but also population-specific correlations which may be caused by environmental and genetic differences. These associations point towards potential interactive metabolic pathways.
authors: Department of Genetics and Pathology, Rudbeck Laboratory, University of Uppsala, SE-75185 Uppsala, Sweden.
authors: Wilmar
authors: W
authors: Igl
authors: Ozren
authors: O
authors: Polašek
authors: Olga
authors: O
authors: Gornik
authors: Ana
authors: A
authors: Knežević
authors: Maja
authors: M
authors: Pučić
authors: Mislav
authors: M
authors: Novokmet
authors: Jennifer
authors: J
authors: Huffman
authors: Carsten
authors: C
authors: Gnewuch
authors: Gerhard
authors: G
authors: Liebisch
authors: Pauline M
authors: PM
authors: Rudd
authors: Harry
authors: H
authors: Campbell
authors: James F
authors: JF
authors: Wilson
authors: Igor
authors: I
authors: Rudan
authors: Ulf
authors: U
authors: Gyllensten
authors: Gerd
authors: G
authors: Schmitz
authors: Gordan
authors: G
authors: Lauc
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002852:Chromatography, Ion Exchange; D005060:Europe; D005260:Female; D054794:Glycomics; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008055:Lipids / Q000097:blood / Q000737:chemistry*; D008297:Male; D008875:Middle Aged; D011134:Polysaccharides / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D055815:Young Adult
chemical_list: D008055:Lipids; D011134:Polysaccharides