title: Norsesquiterpene glycosides in bracken ferns (Pteridium esculentum and Pteridium aquilinum subsp. wightianum) from Eastern Australia: reassessed poisoning risk to animals.
abstract: Austral bracken Pteridium esculentum contains three unstable norsesquiterpene glycosides: ptaquiloside, ptesculentoside, and caudatoside, in variable proportions. The concentration of each of the glycosides was determined in this study as their respective degradation products, pterosin B, pterosin G and pterosin A, by HPLC-UV analysis. Samples of P. esculentum collected from six sites in eastern Australia contained up to 17 mg of total glycoside/g DW, with both ptaquiloside and ptesculentoside present as major components accompanied by smaller amounts of caudatoside. Ratios of ptaquiloside to ptesculentoside varied from 1:3 to 4:3, but in all Australian samples ptesculentoside was a significant component. This profile differed substantially from that of P. esculentum from New Zealand, which contained only small amounts of both ptesculentoside and caudatoside, with ptaquiloside as the dominant component. A similar profile with ptaquiloside as the dominant glycoside was obtained for Pteridium aquilinum subsp. wightianum (previously P. revolutum ) from northern Queensland and also P. aquilinum from European sources. Ptesculentoside has chemical reactivity similar to that of ptaquiloside and presumably biological activity similar to that of this potent carcinogen. The presence of this additional reactive glycoside in Australian P. esculentum implies greater toxicity for consuming animals than previously estimated from ptaquiloside content alone.
authors: Animal Research Institute, Department of Employment, Economic Development and Innovation, Yeerongpilly, Australia. mary.fletcher@deedi.qld.gov.au
authors: Mary T
authors: MT
authors: Fletcher
authors: Ian J
authors: IJ
authors: Brock
authors: Keith G
authors: KG
authors: Reichmann
authors: Ross A
authors: RA
authors: McKenzie
authors: Barry J
authors: BJ
authors: Blaney
mesh_terms: D000818:Animals; D001315:Australia; D002417:Cattle; D002849:Chromatography, Gas / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005517:Foodborne Diseases / Q000662:veterinary*; D006027:Glycosides / Q000032:analysis* / Q000506:poisoning; D058751:Livestock; D010936:Plant Extracts / Q000032:analysis* / Q000506:poisoning; D032498:Pteridium / Q000737:chemistry*; D012717:Sesquiterpenes / Q000032:analysis* / Q000506:poisoning
chemical_list: D006027:Glycosides; D010936:Plant Extracts; D012717:Sesquiterpenes