title: Determination of sodium benzoate by chiral ligand exchange CE based on its inhibitory activity in D-amino acid oxidase mediated oxidation of D-serine.
abstract: A novel quantitative approach for the determination of sodium benzoate (SB) was proposed by the kinetic study about its competitive inhibitory efficiency to D-amino acid oxidase (DAAO) activity with a chiral ligand exchange capillary electrophoresis (CE) method, in which the Zn(II)-L-prolinamide complex was chosen as a novel chiral selector. After the optimization of buffer pH and the chiral selector concentration this chiral ligand exchange CE method was employed to determine labeled D,L-Serine with good linearity (r(2)≥0.995), efficient recovery (95.6-100.9%) and remarkable reproducibility (RSD≤1.2%). This chiral separation method was further used to observe DAAO activity through the determination of D-Serine concentration variation after being incubated with DAAO and obtain the sigmoidal inhibitory curve of SB to DAAO activity. The ascending part of this inhibitory curve was linearly fitted in a limited range for SB from 2.0 to 200 μM with an appropriate coefficient of determination (R(2)=0.990). The linearity was then validated to be a promising method for the analysis of SB with the standout merits of high selectivity and adjustable detection range. Furthermore, this proposed method was used for the pharmacokinetics study of SB.
authors: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing, China.
authors: Haizhi
authors: H
authors: Zhang
authors: Li
authors: L
authors: Qi
authors: Juan
authors: J
authors: Qiao
authors: Lanqun
authors: L
authors: Mao
mesh_terms: D056831:Coordination Complexes / Q000737:chemistry; D003605:D-Amino-Acid Oxidase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008024:Ligands; D010084:Oxidation-Reduction; D011392:Proline / Q000031:analogs & derivatives / Q000737:chemistry; D012694:Serine / Q000737:chemistry*; D020160:Sodium Benzoate / Q000032:analysis*; D013237:Stereoisomerism; D015032:Zinc / Q000737:chemistry
chemical_list: D056831:Coordination Complexes; D008024:Ligands; D012694:Serine; D011392:Proline; D003605:D-Amino-Acid Oxidase; D015032:Zinc; D020160:Sodium Benzoate; C056291:prolinamide