title: Metabolic engineering of menaquinone-8 pathway of Escherichia coli as a microbial platform for vitamin K production.
abstract: Menaquinone-8 (MK-8, vitamin K) is composed of a non-polar side chain and a polar head group. Escherichia coli was chosen and metabolically engineered as a microbial platform for production of MK-8. MK-8 content in E. coli was significantly enhanced by modulating two precursor pools, which supply a non-polar side chain and a polar head group, and further increased by blocking formation of the competitor ubiquinone-8 (Q-8). Overexpression of E. coli IspA, DXR, or IDI increased MK-8 content up to twofold. A similar positive effect was also observed when E. coli MenA, MenB, MenC, MenD, MenE, MenF, or UbiE was overexpressed. The Q-8-deficient ubiCA mutant enhanced MK-8 content by 30% compared to wild-type E. coli. When MenA or MenD was overexpressed, MK-8 content was enhanced fivefold compared with wild-type E. coli.
authors: Department of Molecular Science and Technology, Ajou University, Woncheon-dong, Yeongtong-gu, Suwon 443-749, South Korea.
authors: Min Kyung
authors: MK
authors: Kong
authors: Pyung Cheon
authors: PC
authors: Lee
mesh_terms: D053898:Biosynthetic Pathways / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000096:biosynthesis / Q000235:genetics; D015870:Gene Expression; D005818:Genetic Engineering; D014812:Vitamin K / Q000096:biosynthesis*; D024482:Vitamin K 2 / Q000031:analogs & derivatives* / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; D024482:Vitamin K 2; D014812:Vitamin K; C024223:vitamin MK 8