title: Increased expression of annexin A7 in temporal lobe tissue of patients with refractory epilepsy.
abstract: Annexin A7 is a member of the family of annexins, which are thought to function in the regulation of calcium homeostasis and the fusion of vesicles. Refractory epilepsy may be related to the imbalance of calcium homeostasis. Our aims are to investigate the expression of Annexin A7 in epileptic brains in comparison with human controls and to explore Annexin A7's possible role in refractory epilepsy. We examined the expression of Annexin A7 via immunohistochemistry, double-label immunofluorescence and western blot. The expression of Annexin A7 was shown to be significantly increased in patients with refractory epilepsy. Double-label immunofluorescence and confocal microscopy disclosed Annexin A7 immunoreactivity in the neurons, which were recognized by the antibody of neuron specific enolase (NSE). The result showed that Annexin A7 may be involved in the pathophysiology of refractory epilepsy and may play a role in developing and maintaining the epilepsy.
authors: Department of Neurology, Qilu Hospital, Shandong University, Jinan, China. zhoushengnian126@126.com
authors: Sheng-nian
authors: SN
authors: Zhou
authors: Cheng-shan
authors: CS
authors: Li
authors: Li-qing
authors: LQ
authors: Liu
authors: Yan
authors: Y
authors: Li
authors: Xue-feng
authors: XF
authors: Wang
authors: Lan
authors: L
authors: Shen
mesh_terms: D000293:Adolescent; D000328:Adult; D017310:Annexin A7 / Q000096:biosynthesis*; D001921:Brain / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002648:Child; D004827:Epilepsy / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D006706:Homeostasis; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D018613:Microscopy, Confocal / Q000379:methods; D008875:Middle Aged; D010751:Phosphopyruvate Hydratase / Q000096:biosynthesis; D020033:Protein Isoforms; D013702:Temporal Lobe / Q000378:metabolism*
chemical_list: D017310:Annexin A7; D020033:Protein Isoforms; D010751:Phosphopyruvate Hydratase; D002118:Calcium