title: Antiproliferative activity and cell cycle analysis of 2-(3,5-dihydroxyphenyl)-6-hydroxybenzothiazole on MCF-7 breast and HCT-15 colon cancer cell lines.
abstract: The antiproliferative activity of 2-(3,5-Dihydroxyphenyl)-6-hydroxybenzothiazole (DHB) is reported here. DHB inhibits the growth of human colon cancer HCT-15 with a 50% cell growth inhibition value of 23 μM and breast cancer MCF-7 with a 50% cell growth inhibition value of 41 μM in a dose/time dependent manner by using sulforhodamine B assay. Cell cycle analysis by flow cytometry showed that DHB-induced growth arrest could be associated with apoptosis in both cell lines. Moreover, suppression of clonogenic activity occurs after exposure to DHB at a concentration of 25 μM for HCT-15 and of 40 μM for MCF-7.
authors: Department of Chemistry, Islamic Azad University, Neyshabur Branch, Neyshabur, Iran. m.rajabi.s@gmail.com
authors: Mehdi
authors: M
authors: Rajabi
authors: Filippo
authors: F
authors: Minutolo
authors: Simone
authors: S
authors: Bertini
authors: Marco
authors: M
authors: Macchia
authors: Riccardo
authors: R
authors: Ghidoni
mesh_terms: D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D052160:Benzothiazoles / Q000138:chemical synthesis* / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000473:pathology*; D002453:Cell Cycle / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003110:Colonic Neoplasms / Q000473:pathology*; D003114:Colony-Forming Units Assay; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D013997:Time Factors
chemical_list: C561204:2-(3,5-dihydroxyphenyl)-6-hydroxybenzothiazole; D000970:Antineoplastic Agents; D052160:Benzothiazoles