title: Changes in immunohistochemical levels and subcellular localization after therapy and correlation and colocalization with CD68 suggest a pathogenetic role of Hsp60 in ulcerative colitis.
abstract: In an earlier work, the role of heat shock protein (Hsp60) in the pathogenesis of ulcerative colitis (UC) was suggested by its significant increase in the pathological mucosa parallel with an increase in inflammatory cells. More data in this direction are reported in this work. We analyzed by immunohistochemistry biopsies of colon tissue from 2 groups of patients with UC and treated with either 5-aminosalicylic acid (5-ASA) alone or in combination with a probiotic. We looked for inflammatory markers and Hsp60. Both the treatments were effective in reducing symptoms but the group treated with both 5-ASA and probiotics showed better clinical results. Amelioration of symptoms was associated with reduction of both inflammation and Hsp60, a reduction that was most marked in the group treated with 5-ASA and probiotics. The levels of Hsp60 positively correlated with those of CD68-positive cells, and double immunofluorescence showed a high index of colocalization of the chaperonin and CD68 in lamina propria. Immunoelectron microscopy showed that Hsp60-classically a mitochondrial protein-was abundantly also present in cytosol in biopsies taken at the time of diagnosis, but not after the treatment. Our data suggest that Hsp60 is an active player in pathogenesis of UC and it can be hypothesized that the chaperonin is responsible, at least in part, for initiation and maintenance of disease.
authors: Dipartimento di Chirurgia Generale, d'Urgenza e Trapianti d'Organo, Università degli Studi di Palermo §Istituto Euro-Mediterraneo di Scienza e Tecnologia (IEMEST), Palermo, Italy.
authors: Giovanni
authors: G
authors: Tomasello
authors: Vito
authors: V
authors: Rodolico
authors: Monica
authors: M
authors: Zerilli
authors: Anna
authors: A
authors: Martorana
authors: Fabio
authors: F
authors: Bucchieri
authors: Alessandro
authors: A
authors: Pitruzzella
authors: Antonella
authors: A
authors: Marino Gammazza
authors: Sabrina
authors: S
authors: David
authors: Francesca
authors: F
authors: Rappa
authors: Giovanni
authors: G
authors: Zummo
authors: Provvidenza
authors: P
authors: Damiani
authors: Salvatore
authors: S
authors: Accomando
authors: Manfredi
authors: M
authors: Rizzo
authors: Everly Conway
authors: EC
authors: de Macario
authors: Alberto J L
authors: AJ
authors: Macario
authors: Francesco
authors: F
authors: Cappello
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000009:adverse effects; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D015214:Antigens, Differentiation, Myelomonocytic / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D054316:Biomarkers, Pharmacological / Q000378:metabolism*; D001706:Biopsy; D018834:Chaperonin 60 / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D003093:Colitis, Ulcerative / Q000175:diagnosis / Q000188:drug therapy / Q000276:immunology* / Q000503:physiopathology; D003106:Colon / Q000473:pathology; D018450:Disease Progression; D005500:Follow-Up Studies; D005786:Gene Expression Regulation / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry; D007249:Inflammation; D019804:Mesalamine / Q000008:administration & dosage / Q000009:adverse effects; D009092:Mucous Membrane / Q000187:drug effects / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D019936:Probiotics / Q000008:administration & dosage / Q000009:adverse effects; D021381:Protein Transport; D000097382:CD68 Molecule
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D054316:Biomarkers, Pharmacological; D018834:Chaperonin 60; D019804:Mesalamine; C067980:CD68 antigen, human; D000097382:CD68 Molecule