title: Telomerase-mediated apoptosis of chicken lymphoblastoid tumor cell line by lanthanum chloride.
abstract: To investigate the biological effects of lanthanum on chicken lymphoid tumors, cultures of the MDCC-MSB1 chicken lymphoblastoid cell line were treated with 2.5 mM lanthanum chloride for 2 days at 12-h intervals. The apoptotic cells were detected using the TdT-mediated dUTP nick end labeling assay and flow cytometer analysis. The telomerase activity and the chTERT mRNA expression levels of the MDCC-MSB1 cells were examined. The results showed that MDCC-MSB1 cell apoptosis occurred after incubation for 12 to 48 h induced by 2.5 mM LaCl(3). Consistent with the apoptosis results, telomerase activities in LaCl(3)-treated cells significantly decreased (P < 0.05) compared with 0 h group. Furthermore, the transcription of chTERT gene in MDCC-MSB1 cell was significantly inhibited in LaCl(3) treatment group (P < 0.05). These results suggest that the decrease of the chTERT transcription and telomerase activity play an important role in the La-induced apoptosis in chicken lymphoid tumor.
authors: College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China. zhangziwe@sina.com
authors: Ziwei
authors: Z
authors: Zhang
authors: Jintao
authors: J
authors: Wang
authors: Jinlong
authors: J
authors: Li
authors: Shiwen
authors: S
authors: Xu
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents; D017209:Apoptosis / Q000502:physiology*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002645:Chickens / Q000502:physiology*; D015536:Down-Regulation / Q000187:drug effects; D005434:Flow Cytometry; D020287:In Situ Nick-End Labeling; D007811:Lanthanum / Q000494:pharmacology*; D008223:Lymphoma / Q000188:drug therapy* / Q000473:pathology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D019098:Telomerase / Q000502:physiology*
chemical_list: D000970:Antineoplastic Agents; D012333:RNA, Messenger; C028521:lanthanum chloride; D007811:Lanthanum; D019098:Telomerase