title: Wound microenvironment sequesters adipose-derived stem cells in a murine model of reconstructive surgery in the setting of concurrent distant malignancy.
abstract: BACKGROUND
  It is unclear whether mesenchymal stem cells that are applied to regenerate wound tissues can migrate to existing tumors and enhance their growth. The authors investigated whether adipose-derived stem cells had any effect on the growth and progression of distant tumors when applied to a skin wound.
  
  
  METHODS
  The authors subcutaneously injected murine 4T1 breast cancer cells into all BALB/c-nu/nu mice. After tumor injection, mice were randomized to five groups (five mice per group) based on the means of co-introduction of green fluorescent protein-labeled adipose-derived stem cells, if any. In group 1, adipose-derived stem cells were combined and co-injected subcutaneously. In group 2, they were injected subcutaneously at a distant anatomical site. In group 3, they were injected intravenously. In group 4, they were delivered via a human acellular dermal matrix to a distant skin wound. In group 5, no adipose-derived stem cells were introduced.
  
  
  RESULTS
  After 2 weeks, tumor volume increased in group 1 (356.5 ± 44.4 mm(3)), followed by group 3 (256.6 ± 47.1 mm(3)) and then group 2 (201.6 ± 28.6 mm(3)). In group 4, in which adipose-derived stem cells carried on acellular dermal matrix were applied to a wound distant to the primary tumor, the tumor volume was 143.8 ± 50.9 mm(3), which was similar to that observed in the control group (group 5; 167.8 ± 29.9 mm(3)).
  
  
  CONCLUSIONS
  The authors' findings suggest that the wound microenvironment can retain adipose-derived stem cells, preventing their homing and stromal contribution to a distant neoplastic focus. These findings are an important first step in establishing the feasibility and safety of utilizing adipose-derived stem cell therapy for reconstructive surgery in the setting of malignancy.
authors: Houston, Texas; and Regensburg and Magdeburg, Germany From the Departments of Molecular Pathology and Plastic Surgery, University of Texas M. D. Anderson Cancer Center; Division of Plastic and Reconstructive Surgery, Universitaetsklinikum; and Department of Radiology, Otto von Guericke University.
authors: Andrew M
authors: AM
authors: Altman
authors: Lukas
authors: L
authors: Prantl
authors: Fabian L
authors: FL
authors: Muehlberg
authors: Yao-Hua
authors: YH
authors: Song
authors: Max
authors: M
authors: Seidensticker
authors: Charles E
authors: CE
authors: Butler
authors: Eckhard U
authors: EU
authors: Alt
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000328:Adult; D000818:Animals; D001672:Biocompatible Materials; D003094:Collagen; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D006801:Humans; D007150:Immunohistochemistry; D007275:Injections, Intravenous; D007279:Injections, Subcutaneous; D016086:Lentivirus; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D051379:Mice; D008819:Mice, Nude; D008852:Microradiography; D009336:Necrosis; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000000981:diagnostic imaging / Q000473:pathology / Q000503:physiopathology*; D012867:Skin / Q000293:injuries*; D014162:Transfection; D014945:Wound Healing / Q000502:physiology*
chemical_list: C440568:Alloderm; D001672:Biocompatible Materials; D049452:Green Fluorescent Proteins; D003094:Collagen