title: Abnormal innervation patterns in the anorectum of ETU-induced fetal rats with anorectal malformations.
abstract: To investigate whether anorectal malformations (ARMs) were associated with a global neuromuscular maldevelopment of the lower gastrointestinal (GI) tract and anorectum, the distribution of neuronal markers protein gene product (PGP9.5), nitric oxide synthases (NOs), neuromuscular junction markers (synaptophysin, SYP), interstitial cells of Cajal (ICC) marker (c-kit) within the terminal rectum were analyzed by immunohistochemistry and Western blot in rat embryos with ethylenethiourea (ETU) induced ARMs. From Gestational day16 (Gd16) to Gd21, neural crest-derived cells (NCC) migrated from the proximal gut into the terminal colon, colonising it along its entire length, gradually proliferated and differentiated to innervate the distal gut. From Gd19 to Gd21, significant gross-morphological differences of the anorectum of normal (n=90) and ARMs (n=90) embryos were found. Different myenteric plexus (MPs) development of the anorectum suggested that ARMs were associated with a global abnormal innervation patterns in the anorectum in gestational course and might have some postoperative effect.
authors: Department of Pediatric Surgery, Shengjing Hospital, China Medical University, PR China.
authors: Weilin
authors: W
authors: Wang
authors: Huimin
authors: H
authors: Jia
authors: Hailan
authors: H
authors: Zhang
authors: Qingjiang
authors: Q
authors: Chen
authors: Tao
authors: T
authors: Zhang
authors: Yuzuo
authors: Y
authors: Bai
authors: Zhengwei
authors: Z
authors: Yuan
mesh_terms: D001003:Anal Canal / Q000002:abnormalities / Q000196:embryology / Q000294:innervation*; D000818:Animals; D000071056:Anorectal Malformations; D001006:Anus, Imperforate / Q000139:chemically induced / Q000473:pathology / Q000503:physiopathology; D015415:Biomarkers / Q000378:metabolism; D017615:Enteric Nervous System / Q000002:abnormalities* / Q000196:embryology / Q000378:metabolism; D005260:Female; D005865:Gestational Age; D009197:Myenteric Plexus / Q000002:abnormalities / Q000196:embryology / Q000378:metabolism; D009432:Neural Crest / Q000166:cytology / Q000378:metabolism; D009469:Neuromuscular Junction / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000378:metabolism; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012007:Rectum / Q000002:abnormalities / Q000196:embryology / Q000294:innervation*; D016708:Synaptophysin / Q000378:metabolism; D043222:Ubiquitin Thiolesterase / Q000378:metabolism
chemical_list: D015415:Biomarkers; D016708:Synaptophysin; D019001:Nitric Oxide Synthase; D019009:Proto-Oncogene Proteins c-kit; C489034:UCHL1 protein, rat; D043222:Ubiquitin Thiolesterase