title: Matrine-imprinted monolithic stationary phase for extraction and purification of matrine from Sophorae flavescentis Ait.
abstract: A matrine-imprinted monolithic stationary phase (MIP monolith) was prepared by in situ polymerization for extraction and purification of matrine from Sophorae flavescentis Ait. Matrine was used as the template molecule, methacrylic acid as the function monomer, ethylene glycol dimethacrylate as the cross-linking agent, and toluene and dodecanol as the porogenic solvents. Scanning electron microscope study revealed that a monolithic structure with mesopores and 36 μm diameter nodules was obtained. The molecular recognition process and the effect of varying chromatographic conditions on separation were examined by high-performance liquid chromatography (HPLC). Hydrogen bonding, electrostatic, hydrophobic interactions and the molecular shape matching in MIP monolith cavities were proposed to be responsible for the recognition mechanism. The use of MIP monolith as a solid-phase extraction (SPE) sorbent for extraction and purification of matrine from S. flavescentis Ait was investigated. The extraction yield was 89.2% (for 3.0 mmol l(-1) matrine) with enrichment factor 29.
authors: Faculty of Pharmacy, School of Medicine, Xi'an Jiaotong University, Xi'an, China. fuqiang@mail.xjtu.edu.cn
authors: Qiang
authors: Q
authors: Fu
authors: Qi
authors: Q
authors: Fang
authors: Bianling
authors: B
authors: Feng
authors: Sijuan
authors: S
authors: Sun
authors: Wei
authors: W
authors: Du
authors: Enijian
authors: E
authors: Amut
authors: Aiping
authors: A
authors: Xiao
authors: Chun
authors: C
authors: Chang
mesh_terms: D000179:Acrylates / Q000737:chemistry; D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D008689:Methacrylates / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D054802:Molecular Imprinting / Q000379:methods*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification; D011807:Quinolizines / Q000737:chemistry / Q000302:isolation & purification*; D052616:Solid Phase Extraction / Q000379:methods*; D029909:Sophora / Q000737:chemistry*; D000093842:Matrines
chemical_list: C411824:2-(trifluoromethyl)acrylic acid; D000179:Acrylates; D000470:Alkaloids; D008689:Methacrylates; D010936:Plant Extracts; D011807:Quinolizines; C008384:methacrylic acid; D000093842:Matrines