title: Cloning, expression and purification of the CCN family of proteins in Escherichia coli.
abstract: The CCN proteins are extracellular matrix associated proteins involved in critical cell activities and several aggressive forms of cancer. The proteins share a modular structure of four discrete domains and 38 conserved cysteine residues. The absence of any structural information of these proteins has resulted in a need for the ability to produce substantial amounts of pure CCN protein. Through bacterial expression and inclusion body based purification, pure recombinant CCN proteins have been produced for use in structural and biochemical experiments.
authors: Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath BA2 7AY, UK.
authors: Kenneth P
authors: KP
authors: Holbourn
authors: K Ravi
authors: KR
authors: Acharya
mesh_terms: D055511:CCN Intercellular Signaling Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D017510:Protein Folding; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D055511:CCN Intercellular Signaling Proteins; D011994:Recombinant Proteins