title: A proteomic investigation of Streptococcus agalactiae reveals that human serum induces the C protein β antigen and arginine deiminase.
abstract: Streptococcus agalactiae is a major neonatal pathogen. Disease progression is characterised by bacterial adaptation from commensal maternal vaginal colonisation to environments associated with neonatal disease, including exposure to blood. To explore this adaptation in vitro, we have used proteomics to identify proteins differentially expressed following growth on Todd Hewitt agar in the presence or absence of 10% v/v human serum. Twelve differentially expressed proteins were identified. Notably, the C protein β antigen and arginine deiminase proteins were upregulated following growth in the presence of human serum, consistent with previous studies implicating these two proteins in the pathogenesis of S. agalactiae disease.
authors: School of Life Sciences, University of Northumbria at Newcastle, Newcastle upon Tyne NE1 8ST, UK.
authors: Qian
authors: Q
authors: Yang
authors: Meng
authors: M
authors: Zhang
authors: Dean J
authors: DJ
authors: Harrington
authors: Gary W
authors: GW
authors: Black
authors: Iain C
authors: IC
authors: Sutcliffe
mesh_terms: D000942:Antigens, Bacterial / Q000096:biosynthesis* / Q000097:blood; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000097:blood; D003470:Culture Media; D015180:Electrophoresis, Gel, Two-Dimensional; D054884:Host-Pathogen Interactions; D006801:Humans; D006867:Hydrolases / Q000096:biosynthesis* / Q000097:blood; D020543:Proteome / Q000032:analysis / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D013292:Streptococcus agalactiae / Q000378:metabolism*
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D003470:Culture Media; D020543:Proteome; D006867:Hydrolases; C019723:arginine deiminase