title: Protective effect against oxygen reactive species and skin fibroblast stimulation of Couroupita guianensis leaf extracts.
abstract: In this study, hydroalcoholic leaf extracts of Couroupita guianensis were examined for antioxidant activity, phytochemical and total phenolic composition, stimulation of human skin fibroblast (HSF) proliferation and UV-absorption. The radical scavenging capacity, reducing power and protection against joint oxidation of linoleic acid and β-carotene bleaching oxidation in emulsion were used to evaluate the antioxidant activity. The results of this study strongly indicate in vitro antioxidant activity, which may be due to the presence of a high total phenolic content. In order to identify active principles, the extracts were submitted to fractionation and the compounds isolated were the flavonoids 2',4'-dihydroxy-6'-methoxy-3',5'-dimethylchalcone (1), 7-hydroxy-5-methoxy-6,8-dimethylflavanone (2) and the phenolic acid 4-hydroxybenzoic acid (3). In addition, a high level of stimulation of HSF proliferation and significant absorption of UV radiation were also observed. The results suggest that the hydroalcoholic leaf extracts of C. guianensis have promising skin care properties.
authors: Department of R&D, CarOi'Line Cosmética, S.L., Ponteareas, Spain. ana.lab@caroiline.com
authors: Ana
authors: A
authors: Martínez
authors: Enma
authors: E
authors: Conde
authors: Andrés
authors: A
authors: Moure
authors: Herminia
authors: H
authors: Domínguez
authors: Ramón J
authors: RJ
authors: Estévez
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D047188:Chalcones / Q000737:chemistry / Q000302:isolation & purification; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects* / Q000528:radiation effects; D044950:Flavanones / Q000737:chemistry / Q000302:isolation & purification; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification; D006801:Humans; D031381:Lecythidaceae / Q000737:chemistry*; D015394:Molecular Structure; D010084:Oxidation-Reduction; D010226:Parabens / Q000737:chemistry / Q000302:isolation & purification; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D017382:Reactive Oxygen Species / Q000633:toxicity; D012867:Skin / Q000166:cytology; D014466:Ultraviolet Rays; D019207:beta Carotene / Q000737:chemistry
chemical_list: C501649:2',4'-dihydroxy-6'-methoxy-3',5'-dimethylchalcone; C530120:7-hydroxy-5-methoxy-6,8-dimethylflavanone; D000975:Antioxidants; D047188:Chalcones; D044950:Flavanones; D005419:Flavonoids; D010226:Parabens; D010636:Phenols; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D019207:beta Carotene; C038193:4-hydroxybenzoic acid