title: The inhibitory activity of pomelo essential oil on the bacterial biofilms development on soft contact lenses.
abstract: The aim of present study was to investigate the microbial colonization of worn contact lenses (CLs) and to evaluate the inhibitory effect of pomelo (Citrus maxima) peels essential oil on the biofilm development on unworn CLs. The essential oil was isolated by steam distillation and analyzed by gas chromatography coupled with mass spectrometry, twenty compounds being isolated. The antimicrobial activity of pomelo oil was tested against S. epidermidis and P. aeruginosa strains, known for their ability to develop biofilms on prosthetic devices, by qualitative screening methods and quantitative assay of the minimal inhibitory concentrations (MIC) in order to evaluate the antibiofilm activity. Our study revealed that all worn CLs where 100% colonized by staphylococci and Enterobacteriaceae strains. The pomelo essential oil inhibited the development of bacterial biofilms formed by Gram-positive and Gram-negative microorganisms on soft CLs, its antibiofilm activity being specific and dependent on different physical parameters (contact time and temperature). The architecture of bacterial biofilms developed on soft contact lenses was analyzed using confocal scanning laser microscopy (CSLM).
authors: University of Bucharest, Faculty of Biology, Microbiology Department, Ale. Portocalelor 1-3, 77206, Bucharest, Romania. crina.saviuc@yahoo.com
authors: Crina
authors: C
authors: Saviuc
authors: Luminita
authors: L
authors: Dascălu
authors: Mariana Carmen
authors: MC
authors: Chifiriuc
authors: Valeria
authors: V
authors: Rădulescu
authors: Eliza
authors: E
authors: Oprea
authors: Marcela
authors: M
authors: Popa
authors: Radu
authors: R
authors: Hristu
authors: George
authors: G
authors: Stanciu
authors: Veronica
authors: V
authors: Lazăr
mesh_terms: D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D002957:Citrus / Q000737:chemistry*; D003263:Contact Lenses, Hydrophilic / Q000382:microbiology*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008826:Microbial Sensitivity Tests; D010938:Plant Oils / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000502:physiology; D013212:Staphylococcus epidermidis / Q000187:drug effects / Q000502:physiology
chemical_list: D010938:Plant Oils