title: Genome-wide RNAi screen for viral replication in mammalian cell culture.
abstract: Influenza infections are considered a global threat to public health and cause seasonal epidemics and recurring pandemics. High mutation rates facilitate the generation of viral escape mutants rendering vaccines and drugs directed against virus-encoded targets ineffective. One alternative approach that could prevent viral escape is the targeting of host cell determinants that are temporarily dispensable for the host but crucial for virus replication. Here, we report a genome-wide RNAi screening approach in mammalian cell culture system that led us to the identification of several host cell genes influencing influenza A virus replication. Interestingly, the majority of the identified host gene products are indispensable for viral replication of a broad range of influenza viruses ranging from the highly pathogenic avian H5N1 strain to the current pandemic swine-origin H1N1 strain. Our results provide a new approach to explore virus-host interactions and to identify promising antiviral targets.
authors: Department of Microbiology, Biocenter, University of Würzburg, Würzburg, Germany.
authors: Bhupesh K
authors: BK
authors: Prusty
authors: Alexander
authors: A
authors: Karlas
authors: Thomas F
authors: TF
authors: Meyer
authors: Thomas
authors: T
authors: Rudel
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000378:metabolism; D002460:Cell Line; D017930:Genes, Reporter / Q000235:genetics; D023281:Genomics / Q000379:methods*; D054884:Host-Pathogen Interactions / Q000235:genetics; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D009980:Influenza A virus / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D008156:Luciferases / Q000235:genetics; D019590:Nucleocapsid Proteins; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results; D014758:Viral Core Proteins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism; D014779:Virus Replication / Q000235:genetics*
chemical_list: D000998:Antiviral Agents; C048842:NP protein, Influenza A virus; D019590:Nucleocapsid Proteins; D034741:RNA, Small Interfering; D016601:RNA-Binding Proteins; D014758:Viral Core Proteins; D008156:Luciferases