title: A more efficient method to generate integration-free human iPS cells.
abstract: We report a simple method, using p53 suppression and nontransforming L-Myc, to generate human induced pluripotent stem cells (iPSCs) with episomal plasmid vectors. We generated human iPSCs from multiple donors, including two putative human leukocyte antigen (HLA)-homozygous donors who match ∼20% of the Japanese population at major HLA loci; most iPSCs are integrated transgene-free. This method may provide iPSCs suitable for autologous and allologous stem-cell therapy in the future.
authors: Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan. okita@cira.kyoto-u.ac.jp
authors: Keisuke
authors: K
authors: Okita
authors: Yasuko
authors: Y
authors: Matsumura
authors: Yoshiko
authors: Y
authors: Sato
authors: Aki
authors: A
authors: Okada
authors: Asuka
authors: A
authors: Morizane
authors: Satoshi
authors: S
authors: Okamoto
authors: Hyenjong
authors: H
authors: Hong
authors: Masato
authors: M
authors: Nakagawa
authors: Koji
authors: K
authors: Tanabe
authors: Ken-ichi
authors: K
authors: Tezuka
authors: Toshiyuki
authors: T
authors: Shibata
authors: Takahiro
authors: T
authors: Kunisada
authors: Masayo
authors: M
authors: Takahashi
authors: Jun
authors: J
authors: Takahashi
authors: Hiroh
authors: H
authors: Saji
authors: Shinya
authors: S
authors: Yamanaka
mesh_terms: D044466:Asian People / Q000235:genetics; D018929:Cell Culture Techniques / Q000379:methods*; D018274:Electroporation; D020869:Gene Expression Profiling; D005787:Gene Frequency; D005822:Genetic Vectors; D006680:HLA Antigens / Q000235:genetics; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D014019:Tissue Donors
chemical_list: D006680:HLA Antigens