title: Proteomic comparison of etioplast and chloroplast protein complexes.
abstract: Angiosperms grown in darkness develop etioplasts during skotomorphogenesis. It is well known that etioplasts accumulate large quantities of protochlorophyllideoxidoreductase, are devoid of chlorophyll and are the site to assemble the photosynthetic machinery during photomorphogenesis. Proteomic investigation of the membrane protein complexes by Native PAGE, in combination with CyDye labelling and mass spectrometric analysis revealed that etioplasts and chloroplasts share a number of membrane protein complexes characteristic for electron transport, chlorophyll and protein synthesis as well as fatty acid biosynthesis. The complex regulatory function in both developmental states is discussed.
authors: TRION Pharma GmbH, Munich, Germany. Matthias.ploescher@trionpharma.de
authors: Matthias
authors: M
authors: Plöscher
authors: Veronika
authors: V
authors: Reisinger
authors: Lutz A
authors: LA
authors: Eichacker
mesh_terms: D000595:Amino Acid Sequence; D002734:Chlorophyll / Q000378:metabolism; D060365:Chloroplast Proteins / Q000737:chemistry*; D002736:Chloroplasts / Q000737:chemistry* / Q000235:genetics; D045346:Cytochrome b6f Complex / Q000378:metabolism; D003624:Darkness; D001467:Hordeum / Q000254:growth & development / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000378:metabolism; D040901:Proteomics
chemical_list: D060365:Chloroplast Proteins; D008565:Membrane Proteins; D010940:Plant Proteins; D002734:Chlorophyll; D045346:Cytochrome b6f Complex