title: Effect of hyperthermia on invasion ability and TGF-β1 expression of breast carcinoma MCF-7 cells.
abstract: The present study aimed to investigate heating-induced alterations of breast cancer cell invasion abilities and the potential mechanisms associated with TGF-β1 expression. MCF-7 cells were heated at 43, 45, 47 and 37 °C for 30 min. In vitro cell invasion ability was evaluated by matrigel invasion assay. The activity of matrix metalloproteinase (MMP)-2/9 was investigated by gelatin zymographic assays. Expression of vascular endothelial growth factor (VEGF) and transforming growth factor-β1 (TGF-β1) was investigated by immunocytochemistry and RT-PCR. Apoptosis was analysed by flow-cytometry. The invasive potential of MCF-7 cells was reduced by heating, and MMP-2/9 secretion and enzymatic activity were suppressed. Furthermore, VEGF and TGF-β1 mRNA and proteins were suppressed by hyperthermia. These results suggest that down-regulation of the expression of TGF-β1, EGF and MMPs by hyperthermia probably accounts for the inhibition of the invasive abilities of MCF-7 cells.
authors: Affiliated Tumor Hospital of Guangzhou Medical College, Guangdong 510095, PR China.
authors: Xiaoxue
authors: X
authors: Xie
authors: Xunfan
authors: X
authors: Shao
authors: Fuping
authors: F
authors: Gao
authors: Hekun
authors: H
authors: Jin
authors: Jumei
authors: J
authors: Zhou
authors: Lehui
authors: L
authors: Du
authors: Yingying
authors: Y
authors: Zhang
authors: Weiwei
authors: W
authors: Ouyang
authors: Xiaowen
authors: X
authors: Wang
authors: Lingyun
authors: L
authors: Zhao
authors: Xiaodong
authors: X
authors: Zhang
authors: Jintian
authors: J
authors: Tang
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology*; D005260:Female; D006801:Humans; D006979:Hyperthermia, Induced; D007150:Immunohistochemistry; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis; D009361:Neoplasm Invasiveness / Q000473:pathology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016211:Transforming Growth Factor alpha / Q000096:biosynthesis; D053773:Transforming Growth Factor beta1 / Q000096:biosynthesis*; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis
chemical_list: D016211:Transforming Growth Factor alpha; D053773:Transforming Growth Factor beta1; D042461:Vascular Endothelial Growth Factor A; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9