title: [Development of new nutrient medium for MDCK and Vero cells based on soy hydrolysate obtained using bromeline and assessment of growth characteristics of influenza virus vaccine strains cultivated on them].
abstract: OBJECTIVE
  To develop nutrient medium for MDCK and Vero cells based on soy hydrolysate obtained using bromeline and to assess of growth characteristics of influenza virus vaccine strains cultivated on them.
  
  
  METHODS
  Physico-chemical characteristics of hydrolysate were assessed according to FS 42-3874-99. Growth characteristics of nutrient medium based on soy hydrolysate and vaccine strains of influenza virus A/Solomon Islands/03/06 (H1N1), A/Wisconsin/67/2005 (H3N2) and B/Malaysia/2506/2004 were studied on MDCK and Vero cells.
  
  
  RESULTS
  MDCK and Vero cells grew well on medium based on soy hydrolysate obtained using bromeline with decreased (to 2% and 3% respectively) content of fetal calf serum and allowed effective production of vaccine strains of influenza virus.
  
  
  CONCLUSIONS
  Technology for producing of nutrient medium based on hydrolysate of soy flour obtained using bromeline was developed. This medium could successively used for cultivation of continued cell cultures MDCK and Vero used as substrate for tissue culture-based vaccines against influenza.
authors: N A
authors: NA
authors: Mazurkova
authors: Troshkova
authors: L N
authors: LN
authors: Shishkina
authors: E A
authors: EA
authors: Stavskiĭ
authors: I G
authors: IG
authors: Drozdov
mesh_terms: D000818:Animals; D001963:Bromelains / Q000737:chemistry / Q000494:pharmacology; D002522:Chlorocebus aethiops; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology; D004285:Dogs; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000254:growth & development*; D053122:Influenza A Virus, H3N2 Subtype / Q000254:growth & development*; D007252:Influenza Vaccines / Q000096:biosynthesis*; D014709:Vero Cells
chemical_list: D003470:Culture Media; D007252:Influenza Vaccines; D001963:Bromelains