title: Preparation, characterization, and DNA binding studies of water-soluble quercetin--molybdenum(VI) complex.
abstract: DNA binding studies of flavonoids are needed to understand the reaction mechanism and improve drugs that target DNA. Quercetin (Q) is one of the most common flavonoids that can chelate metal ions and interact with double-stranded DNA. In the present work, UV absorption spectrophotometry, viscosimetry, circular dichroism, and fluorescence spectroscopic techniques were employed to study the interaction of water-soluble quercetin--molybdenum(VI) complex [Q-Mo(VI)] with calf thymus DNA. The binding constants (K(b)) for the complex with DNA were estimated to be 2.9 × 10(3) through spectroscopic titrations. Upon addition of the complex, significant decreases were observed in the viscosity of calf thymus DNA. Circular dichroic spectra indicated that there are certain detectable conformational changes in the DNA double helix when complex was added. Further, competitive methylene blue binding studies with fluorescence spectroscopy have shown that the complex can bind to DNA through nonintercalative mode. The experimental results suggest that Q-Mo(VI) binds to DNA via an outside binding mode.
authors: Department of Plant Biology, Faculty of Natural Science, University of Tabriz, Tabriz, Iran.
authors: Seyed Mojtaba
authors: SM
authors: Ahmadi
authors: Gholamreza
authors: G
authors: Dehghan
authors: Muhammad Ali
authors: MA
authors: Hosseinpourfeizi
authors: Jafar Ezzati Nazhad
authors: JE
authors: Dolatabadi
authors: Soheila
authors: S
authors: Kashanian
mesh_terms: D000818:Animals; D002417:Cattle; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D015394:Molecular Structure; D008982:Molybdenum / Q000737:chemistry*; D011794:Quercetin / Q000138:chemical synthesis / Q000737:chemistry*; D012995:Solubility; D014783:Viscosity; D014867:Water / Q000737:chemistry
chemical_list: D014867:Water; D008982:Molybdenum; D004247:DNA; C427203:calf thymus DNA; D011794:Quercetin