title: miR-126 inhibits proliferation of small cell lung cancer cells by targeting SLC7A5.
abstract: Despite intensive efforts to improve therapies, small cell lung cancer (SCLC) still has a dismal median survival of 18 months. Since miR-126 is under-expressed in the majority of SCLC tumors, we investigated the effect of miR-126 overexpression on the proliferation and cell cycle distribution of H69 cells. Our results demonstrate that miR-126 inhibits proliferation of H69 cells, by delaying the cells in the G1 phase. Short interfering RNA (siRNA) mediated suppression of SLC7A5, a predicted target of mir-126, has the same effect on H69 cells. We also show for the first time that SLC7A5 is a direct target of miR-126.
authors: Dept. of Biochemistry and Molecular Biology, Clinical Genomics Center, University of Debrecen Medical and Health Science Center, Hungary.
authors: Edit
authors: E
authors: Miko
authors: Zoltán
authors: Z
authors: Margitai
authors: Zsolt
authors: Z
authors: Czimmerer
authors: Ildikó
authors: I
authors: Várkonyi
authors: Balázs
authors: B
authors: Dezso
authors: Arpád
authors: A
authors: Lányi
authors: Zsolt
authors: Z
authors: Bacsó
authors: Beáta
authors: B
authors: Scholtz
mesh_terms: D015153:Blotting, Western; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D016193:G1 Phase / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D027283:Large Neutral Amino Acid-Transporter 1 / Q000235:genetics* / Q000378:metabolism; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics*; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055752:Small Cell Lung Carcinoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D013997:Time Factors; D046888:Tissue Array Analysis
chemical_list: D027283:Large Neutral Amino Acid-Transporter 1; C527111:MIRN126 microRNA, human; D035683:MicroRNAs