title: Therapeutic ultrasonic microbubbles carrying paclitaxel and LyP-1 peptide: preparation, characterization and application to ultrasound-assisted chemotherapy in breast cancer cells.
abstract: The aim of this work was to develop a novel targeted drug-loaded microbubble (MB) and to investigate its chemotherapy effect in vitro. Paclitaxel (PTX)-loaded lipid MBs were prepared by a mechanical vibration technique. The LyP-1, a breast tumor homing peptide, was coated onto the surface of PTX-loaded MBs through biotin-avidin linkage. The resulting targeted drug-loaded MBs were characterized and applied to ultrasound-assisted chemotherapy in breast cancer cells. Our results showed the ultrasonic MBs were able to achieve 43%-63% of drug encapsulation efficiency, depending on drug loading amount. The binding affinity assay indicated the attachment of targeted MBs to human MDA-MB-231 breast cancer cells was highly efficient and stable even with ultrasonic irradiation on. The cellular uptake efficiency of payload in targeted MBs was 3.71-, 4.95-, 7.43- and 7.66-fold higher than that of non-targeted MBs at the applied ultrasound time of 30, 60, 90 and 120 s, respectively. In addition, the cell proliferation inhibition assay showed the cell viability of targeted PTX-loaded MBs was significantly lower than that of non-targeted PTX-loaded MBs and non-targeted unloaded MBs when ultrasound was utilized. In conclusion, the study indicated the LyP-1-coated PTX-loaded MBs significantly increased the antitumor efficacy and can be used as a potential chemotherapy approach for ultrasound-assisted breast cancer treatment.
authors: Paul C. Lauterbur Research Center for Biomedical Imaging, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.
authors: Fei
authors: F
authors: Yan
authors: Xiang
authors: X
authors: Li
authors: Qiaofeng
authors: Q
authors: Jin
authors: Chunxiang
authors: C
authors: Jiang
authors: Zidong
authors: Z
authors: Zhang
authors: Tao
authors: T
authors: Ling
authors: Bensheng
authors: B
authors: Qiu
authors: Hairong
authors: H
authors: Zheng
mesh_terms: D001943:Breast Neoplasms / Q000000981:diagnostic imaging / Q000188:drug therapy; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D016503:Drug Delivery Systems; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D045423:Microbubbles / Q000627:therapeutic use*; D017239:Paclitaxel / Q000627:therapeutic use*; D010456:Peptides, Cyclic / Q000627:therapeutic use*; D014465:Ultrasonics; D016217:Ultrasonography, Mammary
chemical_list: C533969:LyP-1 peptide; D010456:Peptides, Cyclic; D017239:Paclitaxel