title: Dereplication coupled with in vitro antioxidant assay of two flavonoid glycosides from Diospyros peregrina fruit.
abstract: Diospyros peregrina is an edible seasonal fruit found in coastal West Bengal, India. The fruits have been reported to possess a significant antioxidant activity. In this study, the aim was to isolate the lead compound responsible for the above-mentioned activity. The aqueous extract of D. peregrina fruit was subjected to dereplication coupled with an in vitro 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging assay. The n-butanol fraction of the aqueous extract of D. peregrina fruit exhibited significant antioxidant activity (IC(50), 131.10 µg mL(-1)) as compared with the parent extract (IC(50), 285.15 µg mL(-1)). The n-butanol fraction was subjected to silica gel column chromatographic separation coupled with a chemo-autographic study of column eluents, employing ethanolic DPPH as a spraying reagent. Two bioactive flavonoid glycosides, namely luteoline-4'-methyl-ether-7-O-glucoside and quercetin-3-O-(glucosyl)-glucoside, were identified to exhibit IC(50) values of 74.04 and 65.78 µg mL(-1), respectively in the DPPH assay.
authors: Department of Pharmaceutical Technology , Advanced Pharmacognosy Research Laboratory, Jadavpur University, Kolkata , India.
authors: Ranabir
authors: R
authors: Sahu
authors: Saikat
authors: S
authors: Dewanjee
authors: Tarun K
authors: TK
authors: Dua
authors: Moumita
authors: M
authors: Gangopadhyay
authors: Anup K
authors: AK
authors: Das
authors: Sankar P
authors: SP
authors: Dey
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D001713:Biphenyl Compounds / Q000737:chemistry; D029788:Diospyros / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D006027:Glycosides / Q000737:chemistry*; D010851:Picrates / Q000737:chemistry
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D005419:Flavonoids; D006027:Glycosides; D010851:Picrates; C004931:1,1-diphenyl-2-picrylhydrazyl