title: In vitro effect of copper chloride exposure on reactive oxygen species generation and respiratory chain complex activities of mitochondria isolated from broiler liver.
abstract: This study is to examine if Cu(2+) can act directly on mitochondria or indirectly by producing reactive oxygen species (ROS), isolated broiler hepatic mitochondria were exposed to different concentrations of Cu(2+) (10, 30, 50 μM). Respiratory chain complex activities, ROS generation, respiratory control ratio (RCR) and mitochondrial membrane potential were investigated. Dose-dependent inhibition of respiratory chain complexes and induction of ROS were observed, which coincided with decreasing RCR both with glutamate + malate or succinate. Further investigation indicated that the membrane potential determined by rhodamine 123 release decreased after CuCl(2) exposure at 30 and 50 μM. In addition, the effects of the antioxidants NAC (200 μM) and GSH (200 μM) were studied at 50 μM Cu(2+). The results indicate that Cu can induce mitochondrial dysfunction in excessive dose and the effect of Cu(2+) exposure on respiratory chain is not site-specific, and antioxidants can protect the mitochondrial function by reducing the formation of free radicals.
authors: College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China.
authors: Rongsheng
authors: R
authors: Su
authors: Rongmei
authors: R
authors: Wang
authors: Shining
authors: S
authors: Guo
authors: Huabin
authors: H
authors: Cao
authors: Jiaqiang
authors: J
authors: Pan
authors: Chengmei
authors: C
authors: Li
authors: Dayou
authors: D
authors: Shi
authors: Zhaoxin
authors: Z
authors: Tang
mesh_terms: D000117:Acetylglucosamine / Q000494:pharmacology; D000818:Animals; D002645:Chickens / Q000378:metabolism*; D003300:Copper / Q000494:pharmacology*; D045304:Cytochromes c / Q000378:metabolism; D004579:Electron Transport / Q000187:drug effects*; D042967:Electron Transport Complex I / Q000378:metabolism; D042963:Electron Transport Complex II / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000378:metabolism; D005260:Female; D005978:Glutathione / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D053078:Membrane Potential, Mitochondrial; D008564:Membrane Potentials / Q000187:drug effects; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D010101:Oxygen Consumption / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D017382:Reactive Oxygen Species; D003300:Copper; D045304:Cytochromes c; D042963:Electron Transport Complex II; D003576:Electron Transport Complex IV; D042967:Electron Transport Complex I; D005978:Glutathione; C029892:cupric chloride; D000117:Acetylglucosamine