title: Accurate fat fraction quantification by multiecho gradient-recalled-echo magnetic resonance at 1.5T in rats with nonalcoholic fatty liver disease.
abstract: OBJECTIVE
  To assess the diagnostic accuracy of a new reconstruction technique for gradient-recalled-echo magnetic resonance (MR) sequences that provides a full decomposition of the water and fat content inside a voxel for nonalcoholic fatty liver disease (NAFLD) in rats.
  
  
  METHODS
  Rats were randomized into two groups. A control group (n = 10) was given free access to regular dry rat chow for 4 weeks. The steatosis (n = 40) group was given free access to feed and water 4 days per week, and fasted for the remaining 3 days for 4 weeks. All rats were killed at 4 weeks and assessed for fatty infiltration and biochemical method.
  
  
  RESULTS
  The average fat content using the gold standard method was 2.65 g (2.20-3.05) of fat/100g liver for the control group and 4.14 g (1.95-8.60) of fat/100g of liver for the overfed group (p<0.05). The average fat-fraction obtained from the MR was 0.016 (0.01-0.02) for the control group and 0.057 (0.00-0.18) for the overfed group. The Pearson correlation coefficient between the samples was r(2) = 0.87.
  
  
  CONCLUSIONS
  Multi-echo MR is a good technique to quantify liver fat in rats.
authors: Hospital Donostia, Department of Gastroenterology. Centro de Investigaciones en Red de Enfermedades Hepáticas y Digestivas, University of Basque Country, Donostia, San Sebastián, Spain. eli.hijonamuruamendiaraz@osakidetza.net
authors: Elizabeth
authors: E
authors: Hijona
authors: Javier
authors: J
authors: Sánchez-González
authors: Jose M
authors: JM
authors: Alústiza
authors: Lander
authors: L
authors: Hijona
authors: Juan
authors: J
authors: Arenas
authors: Elisabeth
authors: E
authors: García
authors: Nohelia
authors: N
authors: Rojas
authors: Maria P
authors: MP
authors: Portillo
authors: Raúl
authors: R
authors: Jiménez
authors: Pablo
authors: P
authors: Aldazabal
authors: Luis
authors: L
authors: Bujanda
mesh_terms: D000273:Adipose Tissue / Q000473:pathology*; D000818:Animals; D005234:Fatty Liver / Q000473:pathology*; D007091:Image Processing, Computer-Assisted / Q000379:methods; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D065626:Non-alcoholic Fatty Liver Disease; D011446:Prospective Studies; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar