title: [Heterologous expression of a synthetic gene encoding a novel hevein-type antimicrobial peptide of Leymus arenarius in Escherichia coli cells].
abstract: A novel antifungal peptide, LAMP-Ia, was isolated from sand-elymus (Leymus arenarius) seeds. Expression of a synthetic gene encoding this peptide in Escherichia coli cells was obtained. The target peptide was expressed as a fusion with thioredoxin. Identity of the recombinant peptide to native LAMP-Ia was confirmed by chromatography, mass spectrometry, and amino acid sequencing. LAMP-Ia displayed a high inhibitory activity in respect of a number of phytopathogenic fungi in in vitro assays, which opens up possibilities for the gene encoding it to be used for genetic transformation of plants and for engineering pathogen-resistant crops.
authors: L L
authors: LL
authors: Utkina
authors: E O
authors: EO
authors: Zhabon
authors: A A
authors: AA
authors: Slavokhotova
authors: E A
authors: EA
authors: Rogozhin
authors: A N
authors: AN
authors: Shiian
authors: E V
authors: EV
authors: Grishin
authors: Ts A
authors: TsA
authors: Egorov
authors: T I
authors: TI
authors: Odintsova
authors: V A
authors: VA
authors: Pukhal'skiĭ
mesh_terms: D000595:Amino Acid Sequence; D023181:Antimicrobial Cationic Peptides / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D001203:Ascomycota / Q000254:growth & development; D005670:Fusarium / Q000254:growth & development; D008969:Molecular Sequence Data; D037121:Plant Lectins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D006109:Poaceae / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics
chemical_list: D023181:Antimicrobial Cationic Peptides; D037121:Plant Lectins; D011994:Recombinant Proteins; C045875:hevein