title: In vivo NMR study of yeast fermentative metabolism in the presence of ferric irons.
abstract: Mathematical modelling analysis of experimental data, obtained with in vivo NMR spectroscopy and 13C-labelled substrates, allowed us to describe how the fermentative metabolism in Saccharomyces cerevisiae, taken as eukaryotic cell model, is influenced by stress factors. Experiments on cellular cultures subject to increasing concentrations of ferric ions were conducted in order to study the effect of oxidative stress on the dynamics of the fermentative process. The developed mathematical model was able to simulate the cellular activity, the metabolic yield and the main metabolic fluxes occurring during fermentation and to describe how these are modulated by the presence of ferric ions.
authors: Department of Pharmaceutical and Applied Chemistry, University of Siena, Via Aldo Moro, 2-53100 Siena, Italy. ricci2@unisi.it
authors: Maso
authors: M
authors: Ricci
authors: Silvia
authors: S
authors: Martini
authors: Claudia
authors: C
authors: Bonechi
authors: Daniela
authors: D
authors: Braconi
authors: Annalisa
authors: A
authors: Santucci
authors: Claudio
authors: C
authors: Rossi
mesh_terms: D002247:Carbon Isotopes / Q000378:metabolism; D005285:Fermentation; D005290:Ferric Compounds / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008954:Models, Biological; D018384:Oxidative Stress / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000378:metabolism* / Q000502:physiology
chemical_list: D002247:Carbon Isotopes; D005290:Ferric Compounds