title: Effect of hyperthermia on the apoptosis and proliferation of CaSki cells.
abstract: Hyperthermia is a promising treatment for human cervical cancer. However, little is known about whether and under what conditions heat treatment exerts tumor inhibition effects on cervical cancer, and the molecular mechanisms behind these cellular responses have yet to be elucidated. We employed the human cervical cancer cell line CaSki as a cellular model and examined the effect of cell apoptosis and proliferation under gradient thermal conditions (43, 45 and 47˚C for 40 min). Heat treatment was found to induce CaSki cell apoptosis and necrosis. Cell cycle analysis showed that cells were arrested in S phase upon the application of hyperthermia, and MTT analysis revealed that cell viability was also reduced. Of the thermal conditions, 45˚C exhibited the best induction of apoptosis, while 47˚C induced direct fierce necrosis. This was further demonstrated by examining the expression level of several key apoptosis-related genes: caspase-3, Smac and Survivin. During apoptosis, caspase-3 and Smac levels were up-regulated, whereas anti-apoptotic Survivin was down-regulated, enhancing programmed cell death. Our results reveal that heating at ≥45˚C induced cell apoptosis and necrosis, and inhibited cell proliferation at both the cellular and molecular levels. These findings support the use of hyperthermia in a clinical setting for the treatment of human cervical cancer.
authors: Oncology Hospital of Hunan Province, Hunan, PR China.
authors: Jumei
authors: J
authors: Zhou
authors: Xiaowen
authors: X
authors: Wang
authors: Lehui
authors: L
authors: Du
authors: Linyun
authors: L
authors: Zhao
authors: Fenglin
authors: F
authors: Lei
authors: Weiwei
authors: W
authors: Ouyang
authors: Yingying
authors: Y
authors: Zhang
authors: Yuping
authors: Y
authors: Liao
authors: Jintian
authors: J
authors: Tang
mesh_terms: D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins; D053148:Caspase 3 / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D006979:Hyperthermia, Induced; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics; D024101:Mitochondrial Proteins / Q000235:genetics; D000077022:Survivin; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000628:therapy*
chemical_list: D051017:Apoptosis Regulatory Proteins; C107489:BIRC5 protein, human; C412403:DIABLO protein, human; D051034:Inhibitor of Apoptosis Proteins; D047908:Intracellular Signaling Peptides and Proteins; D024101:Mitochondrial Proteins; D000077022:Survivin; D053148:Caspase 3