title: The 2-thiouridine unit in the RNA strand is desulfured predominantly to 4-pyrimidinone nucleoside under in vitro oxidative stress conditions.
abstract: The 2-thiouridine (S2U) unit in the RNA strand is predominantly desulfured with H(2)O(2) to 4-pyrimidinone nucleoside (H2U). The resulting H2U-RNA exhibits significantly lower binding affinity to its complementary strand and in certain conditions undergoes strand scission. These results may explain the tRNA loss of biological function in oxidative stress conditions.
authors: Institute of Organic Chemistry, Technical University of Lodz, Zeromskiego 116, 90-924 Lodz, Poland.
authors: Elzbieta
authors: E
authors: Sochacka
authors: Karina
authors: K
authors: Kraszewska
authors: Marek
authors: M
authors: Sochacki
authors: Milena
authors: M
authors: Sobczak
authors: Magdalena
authors: M
authors: Janicka
authors: Barbara
authors: B
authors: Nawrot
mesh_terms: D020029:Base Pairing; D006861:Hydrogen Peroxide / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D011744:Pyrimidinones / Q000378:metabolism; D012343:RNA, Transfer / Q000138:chemical synthesis / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013455:Sulfur / Q000378:metabolism*; D013891:Thiouridine / Q000031:analogs & derivatives* / Q000378:metabolism; D044366:Transition Temperature
chemical_list: C105273:2-thiouridine; D011744:Pyrimidinones; D013891:Thiouridine; D013455:Sulfur; D012343:RNA, Transfer; D006861:Hydrogen Peroxide