title: Nitric oxide production is not required for dihydrosphingosine-induced cell death in tobacco BY-2 cells.
abstract: Sphinganine or dihydrosphingosine (d18:0, DHS), one of the most abundant free sphingoid Long Chain Base (LCB) in plants, is known to induce a calcium dependent programmed cell death (PCD) in tobacco BY-2 cells. In addition, we have recently shown that DHS triggers a production of H2O2, via the activation of NADPH oxidase(s). However, this production of H2O2 is not correlated with the DHS-induced cell death but would rather be associated with basal cell defense mechanisms. In the present study, we extend our current knowledge of the DHS signaling pathway, by demonstrating that DHS also promotes a production of nitric oxide (NO) in tobacco BY-2 cells. As for H2O2, this NO production is not necessary for cell death induction.
authors: Université de Toulouse, UPS, UMR 5546, Laboratoire de Recherche en Sciences Végétales, Castanet-Tolosan, France.
authors: Daniel
authors: D
authors: Da Silva
authors: Christophe
authors: C
authors: Lachaud
authors: Valérie
authors: V
authors: Cotelle
authors: Christian
authors: C
authors: Brière
authors: Sabine
authors: S
authors: Grat
authors: Christian
authors: C
authors: Mazars
authors: Patrice
authors: P
authors: Thuleau
mesh_terms: D001565:Benzoates / Q000494:pharmacology; D016923:Cell Death / Q000187:drug effects; D002478:Cells, Cultured; D007093:Imidazoles / Q000494:pharmacology; D009569:Nitric Oxide / Q000096:biosynthesis*; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000494:pharmacology; D014026:Nicotiana / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism
chemical_list: D001565:Benzoates; D007093:Imidazoles; C079393:1,3-dihydroxy-4,4,5,5-tetramethyl-2-(4-carboxyphenyl)tetrahydroimidazole; D009569:Nitric Oxide; D013110:Sphingosine; C005682:safingol