title: Glucocorticoid receptor expression and antiproliferative effect of dexamethasone on human melanoma cells.
abstract: Glucocorticoids, such as dexamethasone are widely used in cancer therapy and have cell type-specific pro- or antiapoptotic effects. We examined whether melanoma cells are sensitive to dexamethasone treatment. We have demonstrated for the first time that in human melanoma cell lines as well as in benign and malignant melanocytic tumors glucocorticoid receptor (GCR) is present both at mRNA and protein level. Dexamethasone applied at high doses inhibited the in vitro growth of WM983A human melanoma cells. The inhibitory effect was due to apoptosis induction. In the case of this relatively sensitive cell line dexamethasone enhanced the effect of the chemotherapeutic drug DTIC.
authors: Center of Surgical and Molecular Tumor Pathology, National Institute of Oncology, Budapest, Hungary. dobosj76@gmail.com
authors: Judit
authors: J
authors: Dobos
authors: István
authors: I
authors: Kenessey
authors: József
authors: J
authors: Tímár
authors: Andrea
authors: A
authors: Ladányi
mesh_terms: D017209:Apoptosis; D016022:Case-Control Studies; D049109:Cell Proliferation; D003907:Dexamethasone / Q000494:pharmacology*; D005434:Flow Cytometry; D005938:Glucocorticoids / Q000494:pharmacology*; D006801:Humans; D007124:Immunoenzyme Techniques; D008545:Melanoma / Q000188:drug therapy* / Q000378:metabolism* / Q000473:pathology; D009506:Nevus / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D011965:Receptors, Glucocorticoid / Q000235:genetics* / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D005938:Glucocorticoids; D012333:RNA, Messenger; D011965:Receptors, Glucocorticoid; D003907:Dexamethasone