title: Genome-wide identification of transcription start sites yields a novel thermosensing RNA and new cyclic AMP receptor protein-regulated genes in Escherichia coli.
abstract: Intergenic regions often contain regulatory elements that control the expression of flanking genes. Using a deep-sequencing approach, we identified numerous new transcription start sites in Escherichia coli, yielding a new thermosensing regulatory RNA and seven genes previously unknown to be under the control of the global regulator CRP.
authors: Department of Ecology and Evolutionary Biology, Yale University, P.O. Box 27388, West Haven, CT 06516-7388, USA.
authors: Rahul
authors: R
authors: Raghavan
authors: Alan
authors: A
authors: Sage
authors: Howard
authors: H
authors: Ochman
mesh_terms: D001483:Base Sequence; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D021901:DNA, Intergenic; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics* / Q000528:radiation effects; D029968:Escherichia coli Proteins / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D059014:High-Throughput Nucleotide Sequencing; D009690:Nucleic Acid Conformation; D012313:RNA / Q000378:metabolism*; D011953:Receptors, Cyclic AMP / Q000378:metabolism*; D018085:Regulon; D016415:Sequence Alignment; D013696:Temperature; D024363:Transcription Initiation Site
chemical_list: D004269:DNA, Bacterial; D021901:DNA, Intergenic; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D011953:Receptors, Cyclic AMP; C047947:cyclic AMP receptor protein, E coli; D012313:RNA