title: Preparation and characterization of an immunoaffinity column for the selective extraction of salbutamol from pork sample.
abstract: A rapid, simple, and reliable determination method for salbutamol in pork was developed with immunoaffinity column (IAC) extraction followed by HPLC analysis. The salbutamol immunoaffinity column was prepared by coupling CNBr-activated Sepharose-4B with the anti-salbutamol polyclonal antibody which was purified by caprylic acid-ammonium sulfate. The coupling rate of the antibody and Sepharose-4B was 98.6%, and the dynamic column capacity of IAC was 400 ng/mL gel. The average recoveries of salbutamol from spiked pork samples at levels of 2, 10, 20, and 50 ng/g ranged from 83.3% to 92.2%, with the relative standard deviations of 2.8-7.0% (n=5), and the limits of detection and qualification were 0.25 ng/g and 0.5 ng/g, respectively.
authors: Key Laboratory of Biorheological Science and Technology Under Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044, China.
authors: Guomin
authors: G
authors: Wang
authors: Yingguo
authors: Y
authors: Li
authors: Xianliang
authors: X
authors: Li
authors: Xiong
authors: X
authors: Wang
authors: Qi
authors: Q
authors: Guo
authors: Jizong
authors: J
authors: Wu
authors: Cunxian
authors: C
authors: Xi
authors: Zhengguo
authors: Z
authors: Li
mesh_terms: D000420:Albuterol / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D000818:Animals; D055910:Antibodies, Immobilized / Q000378:metabolism; D002846:Chromatography, Affinity / Q000295:instrumentation* / Q000379:methods*; D004350:Drug Residues / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism; D007163:Immunosorbent Techniques / Q000295:instrumentation*; D008460:Meat / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013552:Swine
chemical_list: D055910:Antibodies, Immobilized; D000420:Albuterol