title: Sonic hedgehog stimulates proliferation of human periodontal ligament stem cells.
abstract: Regulation of cell renewal in the periodontium is a critical cell function that has not been clarified. Sonic hedgehog (Shh) is a secreted signaling molecule that plays a key role during development and adult tissue homeostasis. In the present study, we have analyzed the role played by Shh in human periodontal ligament stem cell (HPLSC) proliferation. HPLSC were isolated with anti-STRO-1 antibodies. Shh increased the expression of GLI1 and PTC-1 and selectively stimulated cell proliferation in STRO-1(+) derived from adult periodontal ligament. Shh components were localized to primary cilia in STRO-1(+) cells after Shh stimulation. STRO-1(+) also expressed Shh, suggesting an autocrine-regulated phenomenon. Thus, we propose that Shh plays a critical role in the regulation of cell proliferation in STRO-1(+)/HPLSC.
authors: Facultad de Ciencias, Universidad de Chile, Center for Genomics of the Cell, Las Palmeras 3425, Santiago, Chile.
authors: C
authors: C
authors: Martínez
authors: P C
authors: PC
authors: Smith
authors: J P
authors: JP
authors: Rodriguez
authors: V
authors: V
authors: Palma
mesh_terms: D000293:Adolescent; D000328:Adult; D000954:Antigens, Surface / Q000032:analysis; D019898:Autocrine Communication / Q000502:physiology; D002452:Cell Count; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects; D002469:Cell Separation; D002923:Cilia / Q000648:ultrastructure; D017077:Culture Media, Conditioned; D053823:Hedgehog Proteins / Q000494:pharmacology*; D006801:Humans; D051741:Kruppel-Like Transcription Factors / Q000032:analysis; D009025:Morpholines / Q000494:pharmacology; D009687:Nuclear Proteins / Q000032:analysis; D000072079:Patched Receptors; D010513:Periodontal Ligament / Q000166:cytology* / Q000187:drug effects; D011687:Purines / Q000494:pharmacology; D011956:Receptors, Cell Surface / Q000032:analysis; D043562:Receptors, G-Protein-Coupled / Q000819:agonists / Q000502:physiology; D000072086:Smoothened Receptor; D013234:Stem Cells / Q000187:drug effects*; D014157:Transcription Factors / Q000032:analysis; D055815:Young Adult; D000071676:Zinc Finger Protein GLI1; D000075746:Zinc Finger Protein Gli2
chemical_list: D000954:Antigens, Surface; D017077:Culture Media, Conditioned; C400551:GLI1 protein, human; C082593:GLI2 protein, human; D053823:Hedgehog Proteins; D051741:Kruppel-Like Transcription Factors; D009025:Morpholines; D009687:Nuclear Proteins; D000072079:Patched Receptors; D011687:Purines; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; C509239:SHH protein, human; C491023:SMO protein, human; C525591:STRO-1 antigen, human; D000072086:Smoothened Receptor; D014157:Transcription Factors; D000071676:Zinc Finger Protein GLI1; D000075746:Zinc Finger Protein Gli2; C470893:purmorphamine