title: [Analysis of the surface roughness and bacterium adhesions on the surface of restorative materials in vitro].
abstract: OBJECTIVE
  To observe the common oral bacteria adhesion on the surface of prosthesis with different roughness, and to analyze the sequence of these adhesions and offer the options for better prosthesis and prevention of their occurrence.
  
  
  METHODS
  The specimens made of Co-Cr alloy were polished step by step and divided into six groups. Then, they were put into the liquid BHI culture medium containing Ua, An and Pg. After 24h, the adhesive microbial amount and microbial amount in the culture medium were determined by counting the clonal formation unit. SPSS 6.0 software package was used to analyzed the data.
  
  
  RESULTS
  There was significant difference among different groups of surface roughness(P<0.05). Pg was found neither on the surface of specimen , nor in the liquid BHI culture medium. After 24h, there was significant difference among six groups of Ua and An on the prosthesis surface (P<0.05). The total amount of bacterial of each group was different, too.
  
  
  CONCLUSIONS
  Pg is inhibited when these bacteria are cultivated together. The surface roughness of the materials positively correlates with the amount of adhesive bacteria. We should choose highly polished Co-Cr alloy to lessen the bacteria adhesion. Supported by Research Fund of Science and Technology Commission of Shanghai Municipality (Grant No. 08DZ2271100) and Shanghai Leading Academic Discipline Project (Grant No.T0202).
authors: Department of Prosthodontics, Ninth People's Hospital, College of Stomatology,Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology. Shanghai 200011,China. zhaojun161@126.com
authors: Jun
authors: J
authors: Zhao
authors: Ren-Xiu
authors: RX
authors: Zhang
authors: Yi-Xi
authors: YX
authors: Luo
authors: Yuan-Li
authors: YL
authors: Zheng
mesh_terms: D001422:Bacterial Adhesion; D003764:Dental Materials; D006801:Humans; D066298:In Vitro Techniques; D013499:Surface Properties
chemical_list: D003764:Dental Materials