title: Prediction of conserved microRNAs from skin and mucosal human papillomaviruses.
abstract: Eight human papillomavirus (HPV) types including four cutaneous HPV types (HPV-5, HPV-8, HPV-20 and HPV-38) and four mucosal HPV types (HPV-6, HPV-11, HPV-16 and HPV-18) were selected for this miRNA study. Pre-miRNAs were predicted using a computer programme, and the conserved mature miRNAs were compared to currently known miRNAs. Predicted HPV miRNAs related to miR-466, -467 and -669 were common and specific to the mucosal HPV types. Northern blot hybridization confirmed a predicted miRNA in HPV-positive cervical cancer cell lines encoded by mucosal HPVs. HPV-38 was predicted to express an miRNA conserved to human let-7a and the expression of let-7a, in HPV-38-positive non-melanoma skin cancer (NMSC) biopsies was 10-fold higher than those with HPV-positive (for other types except HPV-38) and HPV-negative NMSCs, suggesting that let-7a expression might be related to HPV-38 infection. Potential gene targets of the predicted miRNA that may aid HPV in infection and pathogenesis were also analysed.
authors: Diamantina Institute for Cancer, Immunology and Metabolic Medicine, Princess Alexandra Hospital, The University of Queensland, Woolloongabba, Brisbane, Australia. w.gu@uq.edu.au
authors: Wenyi
authors: W
authors: Gu
authors: Jiyuan
authors: J
authors: An
authors: Ping
authors: P
authors: Ye
authors: Kong-Nan
authors: KN
authors: Zhao
authors: Annika
authors: A
authors: Antonsson
mesh_terms: D000368:Aged; D001483:Base Sequence; D045744:Cell Line, Tumor; D017124:Conserved Sequence; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D008969:Molecular Sequence Data; D009092:Mucous Membrane / Q000821:virology*; D009690:Nucleic Acid Conformation; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D030361:Papillomavirus Infections / Q000821:virology*; D010802:Phylogeny; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012867:Skin / Q000821:virology*
chemical_list: D035683:MicroRNAs; D012367:RNA, Viral