title: Development of an Si-rhodamine-based far-red to near-infrared fluorescence probe selective for hypochlorous acid and its applications for biological imaging.
abstract: A far-red to near-infrared (NIR) fluorescence probe, MMSiR, based on Si-rhodamine, was designed and synthesized for sensitive and selective detection of HOCl in real time. MMSiR and its oxidized product SMSiR have excellent properties, including pH-independence of fluorescence, high resistance to autoxidation and photobleaching, and good tissue penetration of far-red to NIR fluorescence emission. The value of MMSiR was confirmed by real-time imaging of phagocytosis using a fluorescence microscope. wsMMSiR, a more hydrophilic derivative of MMSiR, permitted effective in vivo imaging of HOCl generation in a mouse peritonitis model. This probe is expected to be a useful tool for investigating the wide range of biological functions of HOCl.
authors: Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors: Yuichiro
authors: Y
authors: Koide
authors: Yasuteru
authors: Y
authors: Urano
authors: Kenjiro
authors: K
authors: Hanaoka
authors: Takuya
authors: T
authors: Terai
authors: Tetsuo
authors: T
authors: Nagano
mesh_terms: D000818:Animals; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D006997:Hypochlorous Acid / Q000032:analysis*; D051379:Mice; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence / Q000379:methods; D009504:Neutrophils / Q000166:cytology; D010587:Phagocytosis; D012235:Rhodamines / Q000737:chemistry*; D017655:Silicon Compounds / Q000737:chemistry*; D013552:Swine
chemical_list: D005456:Fluorescent Dyes; D012235:Rhodamines; D017655:Silicon Compounds; D006997:Hypochlorous Acid