title: A paraptosis-like cell death induced by δ-tocotrienol in human colon carcinoma SW620 cells is associated with the suppression of the Wnt signaling pathway.
abstract: Tocotrienol is considered a beneficial effect agent on inhibition of tumor development. In this study, we focused on the effects of δ-tocotrienol and its possible mechanism on induction of death in human colon cancer SW620 cells. δ-Tocotrienol inhibited proliferation of SW620 cell in a dose-dependent manner. Our findings showed that δ-tocotrienol effectively induced paraptosis-like death in SW620 cells, correlated with the vacuolation that may be from welling and fusion of mitochondria and/or the endoplasmic reticulum (ER) as well as caspase-3 nonactivated. However, there were no changes in apoptosis based on flow cytometry analysis. Of being noted, δ-tocotrienol reduced the expression of β-catenin and wnt-1 proteins by about 50% at the highest dose (20μmol/L). δ-Tocotrienol also decreased cyclin D1, c-jun and MMP-7 protein levels in SW620 cells. Altogether, these data indicate that δ-tocotrienol induces paraptosis-like cell death, which is associated with the suppression of the Wnt signaling pathway. Thus, our findings may provide a novel application in treatment of human colon carcinoma.
authors: Department of Toxicology, Tianjin Center for Disease Control and Prevention, HeDong District, Tianjin 300011, People's Republic of China.
authors: Jing-Shu
authors: JS
authors: Zhang
authors: Da-Ming
authors: DM
authors: Li
authors: Ning
authors: N
authors: He
authors: Ying-Hua
authors: YH
authors: Liu
authors: Chun-Hua
authors: CH
authors: Wang
authors: Shu-Qing
authors: SQ
authors: Jiang
authors: Bing-Qing
authors: BQ
authors: Chen
authors: Jia-Ren
authors: JR
authors: Liu
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D003110:Colonic Neoplasms / Q000188:drug therapy* / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D006801:Humans; D008928:Mitochondria / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D014810:Vitamin E / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D051153:Wnt Proteins / Q000037:antagonists & inhibitors* / Q000187:drug effects; D051155:Wnt1 Protein / Q000037:antagonists & inhibitors
chemical_list: D051153:Wnt Proteins; D051155:Wnt1 Protein; D014810:Vitamin E; C082097:tocotrienol, delta; D053148:Caspase 3