title: Oxidative DNA damage and obesity in type 2 diabetes mellitus.
abstract: OBJECTIVE
  This study illustrates the relationship between oxidative DNA damage and obesity in patients with prediabetes and type 2 diabetes compared with controls.
  
  
  METHODS
  Participants attended the School of Community Health, Diabetes Screening Clinic, Charles Sturt University, Australia, between February 2006 and June 2008. A total of 162 participants (35 type 2 diabetic patients; eight prediabetic subjects; and 119 age-, gender-, and weight-matched controls) were investigated. All patients were selected on clinical grounds.
  
  
  RESULTS
  Serum 8-hydroxy 2'-deoxy-guanosine (8-OHdG) level was significantly greater in the prediabetic subjects (671.3±140 pg/ml) compared with controls (210.1±166 pg/ml; P<0.01). The diabetic group (1979.6±1209 pg/ml) had the highest level of 8-OHdG. There was a significant increase in serum 8-OHdG in obese subjects (848.5±103 pg/ml; P<0.001) and overweight subjects (724±102 pg/ml; P=0.005) compared with the lean subjects (196.5±327 pg/ml).
  
  
  CONCLUSIONS
  Our results indicate that serum 8-OHdG is increased already in prediabetes suggesting oxidative DNA damage to be present with minor elevation of blood glucose levels (BGLs). The statistically significant positive correlation between serum 8-OHdG and body mass index in the diabetic group indicates that obesity has an additive effect to increased BGL contributing to oxidative DNA damage.
authors: Faculty of Science, School of Community Health, Charles Sturt University, PO Box 789 Albury, New South Wales 2640, Australia. halaubaidy@csu.edu.au
authors: Hayder A
authors: HA
authors: Al-Aubaidy
authors: Herbert F
authors: HF
authors: Jelinek
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000368:Aged; D000975:Antioxidants / Q000378:metabolism; D015415:Biomarkers; D001786:Blood Glucose / Q000378:metabolism; D015992:Body Mass Index; D008076:Cholesterol, HDL / Q000097:blood; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000097:blood; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000235:genetics*; D005260:Female; D005609:Free Radicals / Q000378:metabolism; D006442:Glycated Hemoglobin / Q000378:metabolism; D006801:Humans; D008055:Lipids / Q000097:blood; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D009765:Obesity / Q000209:etiology / Q000235:genetics*; D018384:Oxidative Stress / Q000502:physiology*; D011236:Prediabetic State / Q000097:blood / Q000378:metabolism; D012727:Sex Characteristics
chemical_list: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000975:Antioxidants; D015415:Biomarkers; D001786:Blood Glucose; D008076:Cholesterol, HDL; D003849:Deoxyguanosine; D005609:Free Radicals; D006442:Glycated Hemoglobin; D008055:Lipids