title: Dendrimers-delivered short hairpin RNA targeting hTERT inhibits oral cancer cell growth in vitro and in vivo.
abstract: Promising therapeutic application of RNA interference (RNAi) depends on the availability of safe and efficient intracellular delivery systems. Human telomerase reverse transcriptase (hTERT), the catalytic subunit of telomerase complex, is an attractive therapeutic target for oral cancer. Here we investigated the characteristics and anticancer effect of polyamidoamine (PAMAM) dendrimer-mediated short hairpin RNA (shRNA) against hTERT in oral cancer. Dendrimer-mediated shRNA efficiently silenced the hTERT gene in vitro, resulted in cell growth inhibition and apoptosis. Treatment with the shRNA dendriplex attenuated tumor growth in a xenograft model. These studies suggest that RNAi-mediated hTERT gene silencing, coupled with dendrimer delivery, may provide a promising approach for the treatment of oral cancer, in which hTERT is abundantly expressed.
authors: Department of Oral and Maxillofacial Surgery, Guanghua School of Stomatology & Institute of Stomatological Research, Sun Yat-sen University, Guangzhou, Guangdong, China. xiqiangliu@yahoo.com.cn
authors: Xiqiang
authors: X
authors: Liu
authors: Hongzhang
authors: H
authors: Huang
authors: Jianguang
authors: J
authors: Wang
authors: Cheng
authors: C
authors: Wang
authors: Miao
authors: M
authors: Wang
authors: Bin
authors: B
authors: Zhang
authors: Chaobin
authors: C
authors: Pan
mesh_terms: D000818:Animals; D017209:Apoptosis; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000628:therapy*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D050091:Dendrimers / Q000008:administration & dosage; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D018014:Gene Transfer Techniques; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009062:Mouth Neoplasms / Q000235:genetics / Q000628:therapy*; D009368:Neoplasm Transplantation; D010957:Plasmids; D034741:RNA, Small Interfering / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019098:Telomerase / Q000037:antagonists & inhibitors* / Q000235:genetics; D014162:Transfection
chemical_list: D050091:Dendrimers; D034741:RNA, Small Interfering; C509186:TERT protein, human; D019098:Telomerase