title: A multicentre evaluation of the accuracy and performance of IP-10 for the diagnosis of infection with M. tuberculosis.
abstract: IP-10 has potential as a diagnostic marker for infection with Mycobacterium tuberculosis, with comparable accuracy to QuantiFERON-TB Gold In-Tube test (QFT-IT). The aims were to assess the sensitivity and specificity of IP-10, and to evaluate the impact of co-morbidity on IP-10 and QFT-IT. 168 cases with active TB, 101 healthy controls and 175 non-TB patients were included. IP-10 and IFN-γ were measured in plasma of QFT-IT stimulated whole blood and analyzed using previously determined algorithms. A subgroup of 48 patients and 70 healthy controls was tested in parallel with T-SPOT.TB IP-10 and QFT-IT had comparable accuracy. Sensitivity was 81% and 84% with a specificity of 97% and 100%, respectively. Combining IP-10 and QFT-IT improved sensitivity to 87% (p < 0.0005), with a specificity of 97%. T-SPOT.TB was more sensitive than QFT-IT, but not IP-10. Among non-TB patients IP-10 had a higher rate of positive responders (35% vs 27%, p < 0.02) and for both tests a positive response was associated with relevant risk factors. IFN-γ but not IP-10 responses to mitogen stimulation were reduced in patients with TB and non-TB infection. This study confirms and validates previous findings and adds substance to IP-10 as a novel diagnostic marker for infection with M. tuberculosis. IP-10 appeared less influenced by infections other than TB; further studies are needed to test the clinical impact of these findings.
authors: Clinical Research Centre 136, Copenhagen University, Hvidovre Hospital, 2650 Hvidovre, Denmark. mruhwald@gmail.com
authors: Morten
authors: M
authors: Ruhwald
authors: Jose
authors: J
authors: Dominguez
authors: Irene
authors: I
authors: Latorre
authors: Monica
authors: M
authors: Losi
authors: Luca
authors: L
authors: Richeldi
authors: Maria Bruna
authors: MB
authors: Pasticci
authors: Rosanna
authors: R
authors: Mazzolla
authors: Delia
authors: D
authors: Goletti
authors: Ornella
authors: O
authors: Butera
authors: Judith
authors: J
authors: Bruchfeld
authors: Hans
authors: H
authors: Gaines
authors: Irini
authors: I
authors: Gerogianni
authors: Tamara
authors: T
authors: Tuuminen
authors: Giovanni
authors: G
authors: Ferrara
authors: Jesper
authors: J
authors: Eugen-Olsen
authors: Pernille
authors: P
authors: Ravn
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000465:Algorithms; D015415:Biomarkers / Q000378:metabolism; D054357:Chemokine CXCL10 / Q000276:immunology / Q000378:metabolism*; D003718:Denmark / Q000453:epidemiology; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000302:isolation & purification / Q000378:metabolism*; D007558:Italy / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000276:immunology / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D013030:Spain / Q000453:epidemiology; D014374:Tuberculin Test; D014376:Tuberculosis / Q000175:diagnosis* / Q000453:epidemiology / Q000276:immunology / Q000378:metabolism*; D055815:Young Adult
chemical_list: D015415:Biomarkers; C114753:CXCL10 protein, human; D054357:Chemokine CXCL10; D007371:Interferon-gamma