title: Salbutamol improves markers of epithelial function in mice with chronic allergic pulmonary inflammation.
abstract: We investigated the effects of salbutamol on the markers of epithelial function in a murine model of chronic allergic pulmonary inflammation by recording the ciliary beat frequency (CBF) and the transepithelial potential difference (PD) in vivo. Mice were sensitized and received four challenges of ovalbumin (OVA group) or 0.9% saline (control group). Forty-eight hours after the 4th inhalation, we observed eosinophilia in the bronchoalveolar lavage and epithelium remodeling with stored acid mucus in the OVA group (P < 0.001). No difference in the baseline CBF was noticed between the groups; however, the OVA group had a significantly lower baseline PD (P = 0.013). Salbutamol increased the CBF in all groups studied, and the dose response curve to salbutamol increased the PD in the OVA group from 10(-4)M to 10(-2)M. We suggest that salbutamol affects the CBF and the depth of the periciliary layer, which, in great part, determines the ability of the cilia to propel the mucus layer. This effect may have a positive impact on airway mucociliary transport in asthma and may have clinical implications.
authors: Department of Medicine, Laboratory of Experimental Therapeutics, LIM-20, University of São Paulo, Medical School, São Paulo, São Paulo, Brazil.
authors: Alessandra C
authors: AC
authors: Toledo
authors: Fernanda M
authors: FM
authors: Arantes-Costa
authors: Mariângela
authors: M
authors: Macchione
authors: Paulo H N
authors: PH
authors: Saldiva
authors: Elnara M
authors: EM
authors: Negri
authors: Geraldo
authors: G
authors: Lorenzi-Filho
authors: Milton A
authors: MA
authors: Martins
mesh_terms: D056151:Airway Remodeling / Q000187:drug effects; D000420:Albuterol / Q000494:pharmacology*; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D001993:Bronchodilator Agents / Q000494:pharmacology*; D002908:Chronic Disease; D004195:Disease Models, Animal; D004847:Epithelial Cells / Q000187:drug effects; D006967:Hypersensitivity / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D009079:Mucociliary Clearance / Q000187:drug effects*; D011014:Pneumonia / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology
chemical_list: D001993:Bronchodilator Agents; D000420:Albuterol