title: Group-selective molecularly imprinted polymer solid-phase extraction for the simultaneous determination of six sulfonamides in aquaculture products.
abstract: Group-selective molecularly imprinted polymers (MIPs) made from sulfonamides (SAs) using functional monomer methacrylic acid (MAA) were synthesized. The derived molecularly imprinted solid-phase extraction (MISPE) cartridges were developed for the purification and enrichment of aquatic products. The optimum template molecule and the ratio of the functional monomer to the template for obtaining group selectivity to SAs were sulfadimethoxine (SDM) and 4:1, respectively. The MIPs were characterized by Brunauer-Emmett-Teller (BET), scatchard plot, and chromatography analysis, all of which demonstrate better chromatographic behavior and group-selectivity of MIPs for SAs compared with those of corresponding NIPs. The extraction conditions of MISPE for six SAs were optimized; the method precision and accuracy were satisfactory for the fish and shrimp samples at 0.05, 0.1, and 0.2 mg kg(-1) spiked levels. Recoveries ranging from 85.5% to 106.1% (RSD, 1.2-7.0%, n=3) were achieved. The limits of detection (S/N=3) and quantitation (S/N=10) in the shrimp and fish samples were achieved from 8.4 to 10.9 μg kg(-1) and from 22.4 to 27.7 μg kg(-1), respectively. Therefore, the obtained MIPs and MISPE can be employed for the enrichment and clean-up of SAs. This paper presents a new analytical method which enables the simultaneous determination and quantification of SAs in aquaculture products.
authors: Key Laboratory of Applied Technology of Marine Biology, Ministry of Education, Faculty of Life Science and Biotechnology, Ningbo University, Ningbo, PR China. sxzsal78@yahoo.com.cn
authors: Xizhi
authors: X
authors: Shi
authors: Yuan
authors: Y
authors: Meng
authors: Jinghua
authors: J
authors: Liu
authors: Aili
authors: A
authors: Sun
authors: Dexiang
authors: D
authors: Li
authors: Chunxia
authors: C
authors: Yao
authors: Yin
authors: Y
authors: Lu
authors: Jiong
authors: J
authors: Chen
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D000327:Adsorption; D000818:Animals; D017756:Aquaculture; D002851:Chromatography, High Pressure Liquid; D004350:Drug Residues / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D005399:Fishes; D054802:Molecular Imprinting / Q000379:methods*; D033561:Penaeidae; D016062:Porosity; D015203:Reproducibility of Results; D017747:Seafood / Q000032:analysis*; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000379:methods*; D013449:Sulfonamides / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D000097:Acetonitriles; D013449:Sulfonamides; C032159:acetonitrile