title: [Expression of modified oxidase of D-aminoacids of Trigonopsis variabilis in methylotrophic yeasts Pichia pastoris].
abstract: Effective recombinant strains Pichia pastoris that produce functionally active hybrid of Trigonopsis variabilis D-aminoacids bond with chitin-connecting domain of chitinase A1 of Bacillus circulans (DAOcbd) were obtained. The dependence of DAOcbd production levels from production of the number of copies of "expression cassette" integrated in the AOX1 locus of recombinant strains was studied. It was indicated that synthesized DAOcbd may be easily purified and immobilized on chitin sorbents and possessed high specific activity. Produced strains and methods of their cultivation and DAOcbd extraction may be used for development of technologies of obtaining of biocatalyzers in technological processes of obtaining of 7-aminocephalosporane acid.
authors: V A
authors: VA
authors: Redo
authors: E K
authors: EK
authors: Novikova
authors: M A
authors: MA
authors: Él'darov
mesh_terms: D000327:Adsorption; D001407:Bacillus / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D055162:Biocatalysis; D002511:Cephalosporins / Q000096:biosynthesis*; D046528:Chimerism; D002686:Chitin / Q000737:chemistry / Q000378:metabolism; D002688:Chitinases / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D003605:D-Amino-Acid Oxidase / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D018628:Gene Dosage; D015870:Gene Expression; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D004718:Saccharomycetales / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D002511:Cephalosporins; D005656:Fungal Proteins; D011994:Recombinant Proteins; D002686:Chitin; C030735:7-aminocephalosporanic acid; D003605:D-Amino-Acid Oxidase; D002688:Chitinases