title: Ionic liquid-assisted electrospray ionization of polysaccharides.
abstract: In this work, we give the report of significant detection sensitivity improvement of electrospray ionization (ESI) mass spectra of polysaccharides by adding various ionic liquid compounds into samples. Mass spectra obtained were greatly simplified and appeared to be similar to spectra from matrix-assisted laser desorption/ionization due to the narrow charge number distribution. Mass spectra of polysaccharides with the attachment of either anion or cation of ionic liquid compounds were observed. No protonated or deprotonated polysaccharide ions were detected when ionic liquid compounds were added into samples. Little alkali-attached polysaccharide ions were observed. Ionic liquid-assisted ESI (ILA-ESI) mass spectrometry has significantly improved the detection sensitivity of large neutral polysaccharide compounds.
authors: Genomics Research Center, Academia Sinica, Nankang, Taipei, Taiwan.
authors: Yu-Ling
authors: YL
authors: Chang
authors: Yuan-Chun
authors: YC
authors: Lee
authors: Wen-Bin
authors: WB
authors: Yang
authors: Chung-Hsuan
authors: CH
authors: Chen
mesh_terms: D002082:Butylamines / Q000737:chemistry; D005841:Gentisates / Q000737:chemistry; D052578:Ionic Liquids / Q000737:chemistry*; D007554:Isotopes / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry*; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D002082:Butylamines; D005841:Gentisates; D052578:Ionic Liquids; D007554:Isotopes; D011134:Polysaccharides; C010925:2,5-dihydroxybenzoic acid