title: [Study total glucosides of Radix Paeoniae Rubra induced K562 tumor cell apoptosis of signaling pathways and related gene changes].
abstract: OBJECTIVE
  To study the total glucosides of Radix Paeoniae Rubra induced K562 tumor cell apoptosis of signaling pathways and related gene changes.
  
  
  METHODS
  By MTT and flow cytometric, real-time quantitatie polymerase chain reaction (PCR) and Western blot methods researched the level of genes and proteins.
  
  
  RESULTS
  The total glucosides of Radix Paeoniae Rubra could inhibit K562 cell growth by MTT method, there was the relationship of concentration-time between them, TGC prompted K562 cell translocation of phosphatidylserine, may be apoptosis through non-receptor-dependent pathways because caspase-3 mRNA, caspase-9 mRNA and cytochrome C increased, caspase-8 mRNA had no significant change. The expression of Bcl-2 protein and Bcl-X1 protein could decreased, the expression of Bax protein could increased by regulating gene expression.
  
  
  CONCLUSIONS
  TGC induced K562 cell apoptosis that might be through the mitochondria pathway, when cell apoptosis occured, Bcl-2 or Bcl-XI proteins combination of Bax protein would be displacement, then the mitochondrial membrane became permeable to release a series of material, then lead to cell death.
authors: Qiqihar Medical University, Qiqihar 161006, China.
authors: Huiyu
authors: H
authors: Xu
authors: Zhiwei
authors: Z
authors: Chen
authors: Li
authors: L
authors: Zhou
authors: Jianzhao
authors: J
authors: Niu
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics*; D020169:Caspases / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D005960:Glucosides / Q000494:pharmacology*; D006801:Humans; D020014:K562 Cells; D029596:Paeonia / Q000737:chemistry*; D015398:Signal Transduction / Q000187:drug effects*; D013997:Time Factors
chemical_list: D000970:Antineoplastic Agents; D005960:Glucosides; D020169:Caspases