title: A unique N-glycan on human transferrin in CSF: a possible biomarker for iNPH.
abstract: Idiopathic normal pressure hydrocephalus (iNPH) is an elderly dementia caused by abnormal metabolism in the cerebrospinal fluid (CSF). The tap test is the current basis for confirming iNPH, but it shows very low sensitivity, indicating that many patients who might be cured by a shunt operation will be missed. On sodium dodecyl sulfate-polyacrylamide gel electrophoresis, we found two transferrin isoforms: one had a unique N-glycan (Tf-1) whereas the other had N-glycan similar to that of serum transferrin (Tf-2). Glycan analyses revealed that Tf-1 had branching (biantennary) asialo- and agalacto-complex type N-glycans (N-acetylglucosamine [GlcNAc]-terminated glycans), which carried bisecting β1,4-N-acetylglucosamine and core α1,6-fucose. To examine glycoform whether changes in iNPH, we introduced the Tf-2/Tf-1 ratio as a diagnostic index, which minimized blot-to-blot variations in measurement. The Tf-2/Tf-1 ratios of iNPH patients are significantly higher than those of controls (p = 0.0019) and Alzheimer's patients (p = 0.0010). This suggests that the Tf-2/Tf-1 ratio could distinguish iNPH from Alzheimer's disease, and possibly other dementias. In conclusion, glycoform analysis has diagnostic potential in neurological diseases.
authors: Department of Biochemistry, Fukushima Medical University School of Medicine, Fukushima, Japan.
authors: Satoshi
authors: S
authors: Futakawa
authors: Kiyomitsu
authors: K
authors: Nara
authors: Masakazu
authors: M
authors: Miyajima
authors: Atsushi
authors: A
authors: Kuno
authors: Hiromi
authors: H
authors: Ito
authors: Hiroyuki
authors: H
authors: Kaji
authors: Keiro
authors: K
authors: Shirotani
authors: Takashi
authors: T
authors: Honda
authors: Yuriko
authors: Y
authors: Tohyama
authors: Kyoka
authors: K
authors: Hoshi
authors: Yusuke
authors: Y
authors: Hanzawa
authors: Shinobu
authors: S
authors: Kitazume
authors: Rie
authors: R
authors: Imamaki
authors: Katsutoshi
authors: K
authors: Furukawa
authors: Kazuhiro
authors: K
authors: Tasaki
authors: Hiroyuki
authors: H
authors: Arai
authors: Tatsuhiko
authors: T
authors: Yuasa
authors: Masafumi
authors: M
authors: Abe
authors: Hajime
authors: H
authors: Arai
authors: Hisashi
authors: H
authors: Narimatsu
authors: Yasuhiro
authors: Y
authors: Hashimoto
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000134:cerebrospinal fluid; D005260:Female; D006801:Humans; D006850:Hydrocephalus, Normal Pressure / Q000134:cerebrospinal fluid* / Q000175:diagnosis*; D008297:Male; D008875:Middle Aged; D011134:Polysaccharides / Q000134:cerebrospinal fluid* / Q000737:chemistry; D011485:Protein Binding; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014168:Transferrin / Q000134:cerebrospinal fluid* / Q000737:chemistry
chemical_list: D015415:Biomarkers; D011134:Polysaccharides; D014168:Transferrin