title: A lipocentric view of peptide-induced pores.
abstract: Although lipid membranes serve as effective sealing barriers for the passage of most polar solutes, nonmediated leakage is not completely improbable. A high activation energy normally keeps unassisted bilayer permeation at a very low frequency, but lipids are able to self-organize as pores even in peptide-free and protein-free membranes. The probability of leakage phenomena increases under conditions such as phase coexistence, external stress or perturbation associated to binding of nonlipidic molecules. Here, we argue that pore formation can be viewed as an intrinsic property of lipid bilayers, with strong similarities in the structure and mechanism between pores formed with participation of peptides, lipidic pores induced by different types of stress, and spontaneous transient bilayer defects driven by thermal fluctuations. Within such a lipocentric framework, amphipathic peptides are best described as pore-inducing rather than pore-forming elements. Active peptides bound to membranes can be understood as a source of internal surface tension which facilitates pore formation by diminishing the high activation energy barrier. This first or immediate action of the peptide has some resemblance to catalysis. However, the presence of membrane-active peptides has the additional effect of displacing the equilibrium towards the pore-open state, which is then maintained over long times, and reducing the size of initial individual pores. Thus, pore-inducing peptides, regardless of their sequence and oligomeric organization, can be assigned a double role of increasing the probability of pore formation in membranes to high levels as well as stabilizing these pores after they appear.
authors: Instituto de Ciencia Molecular, Universitat de València, C/Catedrático José Beltrán, 2, 46980 Paterna Valencia, Spain.
authors: Gustavo
authors: G
authors: Fuertes
authors: Diana
authors: D
authors: Giménez
authors: Santi
authors: S
authors: Esteban-Martín
authors: Orlando L
authors: OL
authors: Sánchez-Muñoz
authors: Jesús
authors: J
authors: Salgado
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology*; D002463:Cell Membrane Permeability / Q000187:drug effects; D008563:Membrane Lipids / Q000737:chemistry* / Q000378:metabolism; D056004:Molecular Dynamics Simulation; D052899:Pore Forming Cytotoxic Proteins / Q000737:chemistry / Q000494:pharmacology*; D016062:Porosity
chemical_list: D000890:Anti-Infective Agents; D008563:Membrane Lipids; D052899:Pore Forming Cytotoxic Proteins