title: The art and design of functional metagenomic screens.
abstract: This article summarizes general design principles for functional metagenomics. The focus is on Escherichia coli as an expression host, although alternative host-vector systems are discussed in relation to optimizing gene recovery in activity-based screens. Examples of DNA isolation and enrichment approaches, library construction and phenotypic read-out are described with special emphasis on the use of high throughput technologies for rapid isolation of environmental clones encoding phenotypic traits of interest.
authors: Department of Microbiology & Immunology, University of British Columbia, Canada.
authors: Marcus
authors: M
authors: Taupp
authors: Keith
authors: K
authors: Mewis
authors: Steven J
authors: SJ
authors: Hallam
mesh_terms: D004269:DNA, Bacterial; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D015723:Gene Library; D005798:Genes, Bacterial; D005822:Genetic Vectors; D056186:Metagenomics / Q000379:methods*; D059013:Microbial Consortia; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial