title: Boron neutron capture therapy induces cell cycle arrest and DNA fragmentation in murine melanoma cells.
abstract: The melanoma is a highly lethal skin tumor, with a high incidence. Boron Neutron Capture Therapy (BNCT) is a radiotherapy which combines Boron with thermal neutrons, constituting a binary system. B16F10 melanoma and L929 fibroblasts were treated with Boronophenylalanine and irradiated with thermal neutron flux. The electric potential of mitochondrial membrane, cyclin D1 and caspase-3 markers were analyzed. BNCT induced a cell death increase and cyclin D1 amount decreased only in B16F10 melanoma. Besides, there was not caspase-3 phosphorylation.
authors: Biochemical and Biophysical Laboratory, Butantan Institute, São Paulo, Brazil.
authors: F
authors: F
authors: Faião-Flores
authors: P R P
authors: PR
authors: Coelho
authors: J
authors: J
authors: Arruda-Neto
authors: Durvanei A
authors: DA
authors: Maria
mesh_terms: D000818:Animals; D016754:Boron Neutron Capture Therapy; D053148:Caspase 3 / Q000378:metabolism; D059447:Cell Cycle Checkpoints; D045744:Cell Line, Tumor; D053938:DNA Fragmentation; D008546:Melanoma, Experimental / Q000473:pathology / Q000532:radiotherapy*; D051379:Mice
chemical_list: D053148:Caspase 3