title: Influence of the nitrification inhibitor 3,4-dimethylpyrazole phosphate (DMPP) on ammonia-oxidizing bacteria and archaea in rhizosphere and bulk soil.
abstract: In agricultural plant production nitrification inhibitors like 3,4-dimethylpyrazole phosphate (DMPP) are used to retard the microbial nitrification process of fertilized ammonium to enhance the nitrogen supply for cultivated crops and to reduce nitrogen losses from the production system. Besides the well-known ammonia-oxidizing bacteria (AOB) it is known for a few years that also ammonia-oxidizing archaea (AOA) are able to perform the first step in nitrification, hence being also a target for a nitrification inhibitor. However, so far no information are available concerning the effectiveness of DMPP and its extent towards AOB and AOA, neither in bulk soil nor in the root-rhizosphere complex. We investigated in a field experiment performed according to agricultural practice the effect of DMPP on the abundance of AOB and AOA two, four and eight weeks after fertilization. We observed impaired abundances of AOB but not of AOA in both soil compartments that were still visible eight weeks after application, possibly indicating a reduced effectiveness of the nitrification inhibitor in our study.
authors: Helmholtz Zentrum München, German Research Center for Environmental Health, Department of Terrestrial Ecogenetics, Ingolstädter Landstr. 1, 85764 Neuherberg, Germany. kristina.kleineidam@helmholtz-muenchen.de
authors: Kristina
authors: K
authors: Kleineidam
authors: Kristina
authors: K
authors: Košmrlj
authors: Susanne
authors: S
authors: Kublik
authors: Iris
authors: I
authors: Palmer
authors: Helena
authors: H
authors: Pfab
authors: Reiner
authors: R
authors: Ruser
authors: Sabine
authors: S
authors: Fiedler
authors: Michael
authors: M
authors: Schloter
mesh_terms: D000641:Ammonia / Q000032:analysis / Q000378:metabolism*; D001105:Archaea / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D001419:Bacteria / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D009566:Nitrates / Q000032:analysis / Q000378:metabolism; D058465:Nitrification / Q000187:drug effects*; D011720:Pyrazoles / Q000633:toxicity*; D058441:Rhizosphere; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis / Q000378:metabolism / Q000633:toxicity*
chemical_list: C433304:3,4-dimethylpyrazole phosphate; D009566:Nitrates; D011720:Pyrazoles; D012989:Soil Pollutants; D000641:Ammonia