title: Influence of internal fixator flexibility on murine fracture healing as characterized by mechanical testing and microCT imaging.
abstract: Mechanically well-defined stabilization systems have only recently become available, providing standardized conditions for studying the role of the mechanical environment on mouse bone fracture healing. The aim of this study was to characterize the time course of strength recovery and callus development of mouse femoral osteotomies stabilized with either low or high flexibility (in bending and torsion) internal fixation plates. Animals were euthanized and femora excised at 14, 21, and 28 days post-osteotomy for microCT analysis and torsional strength testing. While a larger mineralized callus was observed in osteotomies under more flexible conditions at all time points, the earlier bridging of the mineralized callus under less flexible conditions by 1 week resulted in an earlier recovery of torsional strength in mice stabilized with low flexibility fixation. Ultimate torque values for these bones were significantly higher at 14 and 21 days post-osteotomy compared to bones with the more flexible stabilization. Our study confirms the high reproducibility of the results that are achieved with this new implant system, therefore making it ideal for studying the influence of the mechanical environment on murine fracture healing under highly standardized conditions.
authors: Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Australia. r.steck@qut.edu.au
authors: Roland
authors: R
authors: Steck
authors: Masaki
authors: M
authors: Ueno
authors: Laura
authors: L
authors: Gregory
authors: Noortje
authors: N
authors: Rijken
authors: Martin E
authors: ME
authors: Wullschleger
authors: Moritoshi
authors: M
authors: Itoman
authors: Michael A
authors: MA
authors: Schuetz
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D005264:Femoral Fractures / Q000000981:diagnostic imaging / Q000628:therapy*; D017102:Fracture Healing; D016268:Internal Fixators; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010027:Osteotomy; D018583:Pliability; D055114:X-Ray Microtomography