title: [Construction and confirmation of a recombinant eukaryotic expression plasmid pBABE-hygro-PLAP-1].
abstract: OBJECTIVE
  To construct and confirm a recombinant eukaryotic expression plasmid pBABE-hygro-PLAP-1 containing periodontal ligament-associated protein(PLAP)-1 and a known FLAG sequence.
  
  
  METHODS
  Firstly, the targeted DNA fragment was amplified from pCAG_PLAP-1 by PCR, then PCR product was inserted into p3×FLAG-CMV-10 vector with a known sequence of FLAG. Secondly, plap-1 gene and FLAG were obtained by PCR amplification from p3×FLAG-PLAP-1, then PCR product was inserted to proper sites of vector pBABE-hygro. Finally, the recombinant plasmid pBABE-hygro-PLAP-1 was confirmed by agarose gel electrophoresis and DNA sequence analysis.
  
  
  RESULTS
  The construction of recombinant eukaryotic expression plasmid pBABE-hygro-PLAP-1 was confirmed through restriction enzyme maping analysis and DNA sequencing.
  
  
  CONCLUSIONS
  The recombinant plasmid of pBABE-hygro-PLAP-1 is successfully constructed. It can be applied to plap-1 gene function research in the future. Supported by Natural Science Foundation of Shandong Province (Grant No.2R2010HM050).
authors: Department of Periodontology, School of Stomatology,Shandong Province, China.
authors: Pan-Pan
authors: PP
authors: Zhang
authors: Shu
authors: S
authors: Li
authors: Pi-Shan
authors: PS
authors: Yang
authors: Jing
authors: J
authors: Sun
mesh_terms: D005822:Genetic Vectors; D010513:Periodontal Ligament; D010957:Plasmids