title: Diverse protein kinase interactions identified by protein microarrays reveal novel connections between cellular processes.
abstract: Protein kinases are key regulators of cellular processes. In spite of considerable effort, a full understanding of the pathways they participate in remains elusive. We globally investigated the proteins that interact with the majority of yeast protein kinases using protein microarrays. Eighty-five kinases were purified and used to probe yeast proteome microarrays. One-thousand-twenty-three interactions were identified, and the vast majority were novel. Coimmunoprecipitation experiments indicate that many of these interactions occurred in vivo. Many novel links of kinases to previously distinct cellular pathways were discovered. For example, the well-studied Kss1 filamentous pathway was found to bind components of diverse cellular pathways, such as those of the stress response pathway and the Ccr4-Not transcriptional/translational regulatory complex; genetic tests revealed that these different components operate in the filamentation pathway in vivo. Overall, our results indicate that kinases operate in a highly interconnected network that coordinates many activities of the proteome. Our results further demonstrate that protein microarrays uncover a diverse set of interactions not observed previously.
authors: Department of Genetics, Stanford University School of Medicine, Stanford, California 94305, USA.
authors: Joseph
authors: J
authors: Fasolo
authors: Andrea
authors: A
authors: Sboner
authors: Mark G F
authors: MG
authors: Sun
authors: Haiyuan
authors: H
authors: Yu
authors: Rui
authors: R
authors: Chen
authors: Donald
authors: D
authors: Sharon
authors: Philip M
authors: PM
authors: Kim
authors: Mark
authors: M
authors: Gerstein
authors: Michael
authors: M
authors: Snyder
mesh_terms: D002468:Cell Physiological Phenomena / Q000502:physiology*; D047468:Immunoprecipitation; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D010641:Phenotype; D040081:Protein Array Analysis; D011485:Protein Binding; D011494:Protein Kinases / Q000378:metabolism*; D015203:Reproducibility of Results; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D011494:Protein Kinases; C541984:KSS1 protein, S cerevisiae; D020928:Mitogen-Activated Protein Kinases