title: Frequency-selective rotation of two-particle nanoactuators for rapid and sensitive detection of biomolecules.
abstract: We describe an optomagnetic bionanotechnology for rapid and sensitive solution-based affinity assays. Nanoactuators made from bioactive magnetic nanoparticles undergo rotational motion in the volume of a fluid under frequency-controlled magnetic actuation. The nanoactuators show a time-dependent scattering cross-section to an incoming light beam. We demonstrate that the temporal behavior of the scattered light intensity relates to the number, the magnetic properties and the size distribution of the nanoactuators, independently revealing the average value and variation in the magnetic properties of the nanoparticles as well as the concentration of nanoactuators. The method is applied to detect biomolecules in fluid by interparticle binding. In a total assay time of less than 3 min, we demonstrate a limit of detection lower than 400 fM in buffer and 5 pM in human plasma.
authors: Philips Research, High Tech Campus, Eindhoven, The Netherlands.
authors: Andrea
authors: A
authors: Ranzoni
authors: Jean J H B
authors: JJ
authors: Schleipen
authors: Leo J
authors: LJ
authors: van Ijzendoorn
authors: Menno W J
authors: MW
authors: Prins
mesh_terms: D015374:Biosensing Techniques; D002021:Buffers; D016000:Cluster Analysis; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008027:Light; D008280:Magnetics; D053768:Metal Nanoparticles / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D010316:Particle Size; D012542:Scattering, Radiation; D013997:Time Factors
chemical_list: D002021:Buffers