title: Nucleostemin affects the proliferation but not differentiation of oral squamous cell carcinoma cells.
abstract: Nucleostemin (NS) has been reported as essential for stem and cancer cell proliferation. To investigate the significance of NS in oral squamous cell carcinomas (OSCCs), we examined NS expression in neoplastic tissue of the tongue and in OSCC cell lines. Nucleostemin expression in the histological samples showed positive correlation with Ki-67 expression. Furthermore, NS expression was associated with cellular proliferation in OSCC cell lines using siRNA, which upregulated p27, a cyclin-dependent kinase inhibitor. Regarding OSCC differentiation, NS expression did not influence cornification or oral epithelial differentiation markers such as involucrin and cytokeratin19. Thus, NS is widely expressed in normal and neoplastic oral epithelial tissues, and is likely a marker of proliferation.
authors: Department of Pathology and Experimental Medicine, Kumamoto University Graduate School of Medical Sciences, Kumamoto, Japan.
authors: Ryoji
authors: R
authors: Yoshida
authors: Tetsuhiro
authors: T
authors: Fujimoto
authors: Shinji
authors: S
authors: Kudoh
authors: Masashi
authors: M
authors: Nagata
authors: Hideki
authors: H
authors: Nakayama
authors: Masanori
authors: M
authors: Shinohara
authors: Takaaki
authors: T
authors: Ito
mesh_terms: D002118:Calcium / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D019204:GTP-Binding Proteins / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry; D053539:Keratin-19 / Q000032:analysis; D019394:Ki-67 Antigen / Q000032:analysis; D009062:Mouth Neoplasms / Q000473:pathology*; D009687:Nuclear Proteins / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis; D034741:RNA, Small Interfering / Q000235:genetics
chemical_list: C469949:GNL3 protein, human; D053539:Keratin-19; D019394:Ki-67 Antigen; D009687:Nuclear Proteins; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D019204:GTP-Binding Proteins; D002118:Calcium