title: Spatiotemporal expression of Wnt5a during the development of the hindgut and anorectum in human embryos.
abstract: OBJECTIVE
  The aim of the study was to determine the spatiotemporal expression of Wnt5a during hindgut and anorectum development in human embryos and to explore the possible role of Wnt5a during the morphogenesis of the human hindgut and anorectum.
  
  
  METHODS
  The embryos (n = 107) were sectioned serially and sagittally, using Wnt5a immunohistochemical staining on the caudal midline from the 4th-9th weeks of gestation.
  
  
  RESULTS
  From the 4th-7th week of gestation, the Wnt5a-positive cells were mainly located on the epithelium of the apical urorectal septum, hindgut, and cloacal membrane. After the anorectum and the urogenital sinus (UGS) opened to the amniotic cavity during the 7th week, the Wnt5a-positive cells disappeared and remained negative up to the 9th week on the epithelium of the anal canal.
  
  
  CONCLUSIONS
  The expression of Wnt5a was constantly active during human hindgut and anorectum development and disappeared after the anus formed, suggesting that Wnt5a plays an important role in human hindgut and anorectal morphogenesis.
authors: Department of Pediatric Surgery, Shengjing Hospital, China Medical University, No. 36 Sanhao Street, Heping District, Shenyang, People's Republic of China, 110004.
authors: Fei Fei
authors: FF
authors: Li
authors: Tao
authors: T
authors: Zhang
authors: Yu Zuo
authors: YZ
authors: Bai
authors: Zheng Wei
authors: ZW
authors: Yuan
authors: Wei Lin
authors: WL
authors: Wang
mesh_terms: D001003:Anal Canal / Q000166:cytology / Q000196:embryology* / Q000378:metabolism*; D002988:Cloaca / Q000166:cytology / Q000378:metabolism; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000378:metabolism; D012007:Rectum / Q000166:cytology / Q000196:embryology* / Q000378:metabolism*; D013194:Staining and Labeling; D013997:Time Factors; D051153:Wnt Proteins / Q000235:genetics* / Q000378:metabolism; D000071818:Wnt-5a Protein
chemical_list: D011518:Proto-Oncogene Proteins; C085389:WNT5A protein, human; D051153:Wnt Proteins; D000071818:Wnt-5a Protein