title: Proteomics reveals potential biomarkers of seed vigor in sugarbeet.
abstract: To unravel biomarkers of seed vigor, an important trait conditioning crop yield, a comparative proteomic study was conducted with sugarbeet seed samples of varying vigor as generated by an invigoration treatment called hydropriming and an aging treatment called controlled deterioration. Comparative proteomics revealed proteins exhibiting contrasting behavior between seed samples. Thus, 18 proteins were up-regulated during priming and down-regulated during aging and further displayed an up-regulation upon priming of the aged seeds, meaning that down-regulation of these spot volumes during aging was reversible upon subsequent priming. Also, 11 proteins exhibited the converse behavior characterized by a decrease and an increase of the spot volumes during priming and aging of the control seeds, respectively, and a decrease in the spot volumes upon priming of the aged seeds. The results underpinned the role in seed vigor of several metabolic pathways involved in lipid and starch mobilization, protein synthesis or the methyl cycle. They also corroborate previous studies suggesting that the glyoxylate enzyme isocitrate lyase, the capacity of protein synthesis and components of abscisic acid signaling pathways are likely contributors of seed vigor.
authors: CNRS/UCBL/INSA/Bayer CropScience Joint Laboratory (UMR), Lyon, France.
authors: Julie
authors: J
authors: Catusse
authors: Juliane
authors: J
authors: Meinhard
authors: Claudette
authors: C
authors: Job
authors: Jean-Marc
authors: JM
authors: Strub
authors: Uwe
authors: U
authors: Fischer
authors: Elena
authors: E
authors: Pestsova
authors: Peter
authors: P
authors: Westhoff
authors: Alain
authors: A
authors: Van Dorsselaer
authors: Dominique
authors: D
authors: Job
mesh_terms: D027461:Beta vulgaris / Q000254:growth & development / Q000378:metabolism*; D015415:Biomarkers / Q000032:analysis* / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D018525:Germination; D007522:Isocitrate Lyase / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D012639:Seeds / Q000254:growth & development / Q000378:metabolism*; D013997:Time Factors
chemical_list: D015415:Biomarkers; D007522:Isocitrate Lyase