title: Distribution of ten antibiotic resistance genes in E. coli isolates from swine manure, lagoon effluent and soil collected from a lagoon waste application field.
abstract: The prevalence of ten antibiotic resistance genes (ARGs) was evaluated in a total of 616 Escherichia coli isolates from swine manure, swine lagoon effluent, and from soils that received lagoon effluent on a commercial swine farm site in Sampson County, North Carolina (USA). Isolates with ARGs coding for streptomycin/spectinomycin (aadA/strA and strB), tetracycline (tetA and tetB), and sulfonamide (sul1) occurred most frequently (60.6-91.3%). The occurrence of E. coli isolates that carried aadA, tetA, tetB, and tetC genes was significantly more frequent in soil samples (34.0-97.2%) than in isolates from lagoon samples (20.9-90.6%). Furthermore, the frequency of isolates that contain genes coding for aadA and tetB was significantly greater in soil samples (82.6-97.2%) when compared to swine manure (16.8-86.1%). Isolates from the lagoon that carried tetA, tetC, and sul3 genes were significantly more prevalent during spring (63.3-96.7%) than during winter (13.1-67.8%). The prevalence of isolates from the lagoon that possessed the strA, strB, and sul1 resistance genes was significantly more frequent during the summer (90.0-100%) than during spring (66.6-80.0%). The data suggest that conditions in the lagoon, soil, and manure may have an impact on the occurrence of E. coli isolates with specific ARGs. Seasonal variables seem to impact the recovery isolates with ARGs; however, ARG distribution may be associated with mobile genetic elements or a reflection of the initial numbers of resistant isolates shed by the animals.
authors: Department of Soil Science, North Carolina State University, Campus Box 7619, Raleigh, NC 27695-7619, USA. Alexandria_Graves@Ncsu.Edu
authors: A K
authors: AK
authors: Graves
authors: L
authors: L
authors: Liwimbi
authors: D W
authors: DW
authors: Israel
authors: E
authors: E
authors: van Heugten
authors: B
authors: B
authors: Robinson
authors: C W
authors: CW
authors: Cahoon
authors: J F
authors: JF
authors: Lubbers
mesh_terms: D000818:Animals; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D029968:Escherichia coli Proteins / Q000235:genetics; D005798:Genes, Bacterial; D008372:Manure / Q000382:microbiology*; D009657:North Carolina; D012621:Seasons; D012988:Soil Microbiology; D013552:Swine; D014871:Water Microbiology
chemical_list: D029968:Escherichia coli Proteins; D008372:Manure