title: Chronic intermittent hypoxia-induced augmented cardiorespiratory outflow mediated by vasopressin-V₁A receptor signaling in the medulla.
abstract: A co-morbidity of sleep-disordered breathing is hypertension associated with elevated sympathetic nerve activity, which may result from chronic intermittent hypoxia (CIH). CIH evokes plasticity in cardiorespiratory regulating sites, including the paraventricular nucleus (PVN), which acts to sustain increased sympathetic nerve activity. Our working hypothesis is that vasopressin neurons mediate the sustained increase in blood pressure and altered breathing associated with CIH. In a series of neuroanatomical experiments, we determined if vasopressin-containing PVN neurons innervate rostral ventrolateral medulla (RVLM), and altered cardiorespiratory responses induced by CIH conditioning (8h/day for 10 days) is mediated by vasopressin-V(1A ) receptor signaling in the medulla. In the first set of experiments, cholera toxin β subunit was microinjected into the RVLM to delineate innervation of the PVN. Immunohistochemistry data showed vasopressin-containing PVN neurons were double-labeled with cholera toxin β subunit, indicating vasopressin projection to the RVLM. In the second set, sections of the medulla were immunolabeled for vasopressin V(1A ) receptor, and its expression was significantly higher in the RVLM and in the neighboring rostral ventral respiratory column in CIH- than from RA-conditioned rats. In a series of physiological experiments,we determined if blocking the vasopressin V(1A )receptor in the medulla would normalize blood pressure in CIH-conditioned rats and also attenuate the evoked responses to PVN disinhibition.Blood pressure, heart rate, diaphragmatic and genioglossus muscle activity were recorded in anesthetized, ventilated and vagotomized rats. The PVN was disinhibited by microinjecting bicuculline before and after blocking vasopressin V(1A ) receptors in the RVLM/rostral ventral respiratory column. In RA-conditioned rats, PVN disinhibition increased blood pressure, heart rate, minute diaphragmatic and genioglossus muscle activity, and these increases were attenuated after blocking the vasopressin V(1A ) receptor. In CIH-conditioned rats, a significantly greater dose of blocker was required to blunt these physiological responses and it also normalized the baseline blood pressure. Our findings indicate that vasopressin is the neuropeptide released from PVN neurons that modulates cardiorespiratory output via the RVLM and rostral ventral respiratory column.
authors: Department of Pediatrics, Case Western Reserve University, Cleveland, OH 44106, USA. prabha.kc@case.edu
authors: Kc
authors: K
authors: Prabha
authors: Kannan V
authors: KV
authors: Balan
authors: Richard J
authors: RJ
authors: Martin
authors: Joseph C
authors: JC
authors: Lamanna
authors: Musa A
authors: MA
authors: Haxhiu
authors: Thomas E
authors: TE
authors: Dick
mesh_terms: D000818:Animals; D001640:Bicuculline / Q000494:pharmacology; D001794:Blood Pressure / Q000187:drug effects; D002908:Chronic Disease; D003964:Diaphragm; D058787:GABA-A Receptor Antagonists / Q000494:pharmacology; D006339:Heart Rate; D006973:Hypertension; D000860:Hypoxia / Q000503:physiopathology*; D008526:Medulla Oblongata / Q000378:metabolism*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D010286:Paraventricular Hypothalamic Nucleus / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017483:Receptors, Vasopressin / Q000378:metabolism*; D015398:Signal Transduction; D012891:Sleep Apnea Syndromes / Q000503:physiopathology*; D014667:Vasopressins / Q000378:metabolism
chemical_list: D058787:GABA-A Receptor Antagonists; D017483:Receptors, Vasopressin; D014667:Vasopressins; D001640:Bicuculline