title: Enhancement of recombinant human BMP-7 bone formation with bmp binding peptide in a rodent femoral defect model.
abstract: Bone morphogenetic binding peptide (BBP) is an 18.5 kDa fragment of a bone matrix protein peptide. A rat femoral defect model was used to test the effect of BBP combined with recombinant human bone morphogenetic protein-7 (rhBMP-7) to induced bone healing. Two doses of BBP (500 and 1000 µg) were tested with two doses of rhBMP-7 (2 and 5 µg), and the results were compared with a positive control (10 µg rhBMP-7). Bone healing was evaluated by radiology, manual palpation, microcomputed tomography, and histology. The high dose of 10 µg of rhBMP-7 resulted in a consistent 100% bone union rate and a mature histological appearance on histology, and was used as a positive control. When 1000 µg of BBP was combined with lower doses of BMP-7 (2 µg rhBMP-7 or 5 µg rhBMP-7) significant differences were seen in radiographic scores, manual palpation, and bone volume, when compared to 2 µg rhBMP-7 or 5 µg rhBMP-7 alone. The combination of 1000 µg of BBP and 5 µg rhBMP-7 also achieved 100% fusion rate, induced a larger amount of bone formation, and yielded similar maturity of bone marrow when compared with the high dosage 10 µg rhBMP-7 group. This study demonstrated that when combined together, BBP can enhance the bone healing of rhBMP-7. Improved healing imparted by the addition of BBP may result in lesser amounts of rhBMP-7 needed to achieve union in the clinical setting.
authors: Department of Orthopedics Surgery, Chang Gung Memorial Hospital, Chang Gung University, Taoyuan, Taiwan.
authors: Jen-Chung
authors: JC
authors: Liao
authors: Shiau-Tzu
authors: ST
authors: Tzeng
authors: Gun
authors: G
authors: Keorochana
authors: Kwang-Bok
authors: KB
authors: Lee
authors: Jared S
authors: JS
authors: Johnson
authors: Yuichiro
authors: Y
authors: Morishita
authors: Samuel S
authors: SS
authors: Murray
authors: Jeffrey C
authors: JC
authors: Wang
mesh_terms: D000818:Animals; D055419:Bone Morphogenetic Protein 7 / Q000494:pharmacology*; D004357:Drug Synergism; D005269:Femur / Q000293:injuries* / Q000473:pathology; D017102:Fracture Healing / Q000187:drug effects*; D006801:Humans; D008297:Male; D023421:Models, Animal; D010446:Peptide Fragments / Q000494:pharmacology*; D051381:Rats; D011917:Rats, Inbred Lew; D011994:Recombinant Proteins / Q000494:pharmacology*; D055114:X-Ray Microtomography
chemical_list: C525819:BMP7 protein, human; D055419:Bone Morphogenetic Protein 7; D010446:Peptide Fragments; D011994:Recombinant Proteins; C558392:bone morphogenetic binding peptide, human