title: Synthesis of stable cadmium sulfide nanoparticles using surfactin produced by Bacillus amyloliquifaciens strain KSU-109.
abstract: In this study, a surfactin was extracted from a novel surfactant producing bacterial strain Bacillus amyloliquifaciens KSU-109, isolated from rhizosphere of date palm (Phoenix dactylifera), and characterized based on 16Sr RNA and sfp genes using Blastn, Blastx and phylogenetic analyses. The study was performed to obtain a renewable bioresource for surfactin production, and its application in nanotechnology as a non-hazardous and environmentally compatible nanoparticle (NP) stabilizer. The strain KSU-109 produced the surfactin with an average yield of 160 mg/L with strong surfactant activity, reducing the surface tension of the medium from 72 mN/m to 29.3 mN/m. The surfactin preparation was used for synthesizing the cadmium sulfide nanoparticles (CdS-NPs) by mixing 0.005% surfactin with 1mM Cd(NO(3))(2) in 1:1 ratio (v/v) and 10mM Na(2)S solution at pH 7.2 and ambient temperature, which were stable up to 120 days. The surfactin stabilized CdS-NPs were characterized using XRD, TEM, and spectroscopic techniques. The data revealed a significant role of surfactin as a stabilizer and capping agent, which also causes phase transition to yield the cubic/hexagonal CdS-NPs of average size of 3-4 nm. The results elucidated the significance of biocompatible and biodegradable surfactin as an effective and inexpensive stabilizing agent for developing stable CdS nanoparticles.
authors: Al-Jeraisy Chair for DNA Research, Department of Zoology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
authors: Braj Raj
authors: BR
authors: Singh
authors: Sourabh
authors: S
authors: Dwivedi
authors: Abdulaziz A
authors: AA
authors: Al-Khedhairy
authors: Javed
authors: J
authors: Musarrat
mesh_terms: D001407:Bacillus / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D019187:Cadmium Compounds / Q000737:chemistry*; D055666:Lipopeptides / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010456:Peptides, Cyclic / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013053:Spectrophotometry / Q000379:methods; D013440:Sulfides / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry / Q000378:metabolism; D017855:Transferases (Other Substituted Phosphate Groups) / Q000235:genetics; D014961:X-Ray Diffraction
chemical_list: D001426:Bacterial Proteins; D019187:Cadmium Compounds; D055666:Lipopeptides; D010456:Peptides, Cyclic; D012336:RNA, Ribosomal, 16S; D013440:Sulfides; D013501:Surface-Active Agents; C082662:phosphopantetheinyl transferase; C034939:cadmium sulfide; C009365:surfactin peptide; D017855:Transferases (Other Substituted Phosphate Groups)