title: Effects of Ficus hirta Vahl. (Wuzhimaotao) extracts on growth inhibition of HeLa cells.
abstract: Ficus hirta Vahl. (Wuzhimaotao) is widely used as a folk medicine by Hakka people in southern China. In order to ascertain if any major fraction can be attributed to have pronounced anticancer effect, extracts of Wuzhimaotao on cytotoxic and apoptosis of HeLa cell lines were evaluated. HeLa cells were cultured and incubated with different concentrations of crude aqueous extracts (CAE), ethyl acetate extracts (EAE), and butyl alcohol extracts (BAE). It showed CAE, EAE, and BAE decreased cell viability on HeLa cells as a dose-dependent manner, and induced a sub-G1 peak in flow cytometry histogram of treated cells compared to the control. Apoptotic cell death is involved in CAE, EAE, and BAE toxicity, with EAE having a significant decrease in G1 population. An over all analysis of results showed that Wuzhimaotao extracts exert antiproliferative action and growth inhibition on HeLa cells through apoptosis induction which indicates its anticancer properties and deserves further investigation.
authors: College of Life Science, Guangdong Key Lab of Biotechnology for Plant Development, South China Normal University, Guangzhou 510631, Guangdong, China.
authors: You-wei
authors: YW
authors: Zeng
authors: Xiao-zhen
authors: XZ
authors: Liu
authors: Zhen-cheng
authors: ZC
authors: Lv
authors: Yong-hong
authors: YH
authors: Peng
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D000440:Butanols / Q000737:chemistry; D018929:Cell Culture Techniques; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000431:Ethanol / Q000737:chemistry; D030681:Ficus / Q000737:chemistry*; D005434:Flow Cytometry; D006367:HeLa Cells; D006801:Humans; D018517:Plant Roots / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D000440:Butanols; D004365:Drugs, Chinese Herbal; D014867:Water; D000431:Ethanol