title: Investigation on CAST, CAPN1 and CAPN3 porcine gene polymorphisms and expression in relation to post-mortem calpain activity in muscle and meat quality.
abstract: This study aimed to detect variability in CAST, CAPN1 and CAPN3 porcine genes and to investigate the effect of CAST and CAPN1 polymorphisms on the activity of native and autolyzed μ-calpain and m-calpain, measured from 1 to 72 h post-mortem in Longissimus dorsi (LD) muscle of 30 pigs. Effects of polymorphisms on meat quality parameter such as pH, color and drip loss were also evaluated. Samples carrying CAST EU137105:g.76,872AA genotype showed higher autolyzed μ-calpain activity 24 and 72 h post-mortem, as well as lower drip loss values. Expression of CAST, CAPN1 and CAPN3 was assessed in LD muscles divergent for shear force. Higher CAST and CAPN3 expression was found in LD with high shear force (P<0.2), confirming a direct role for calpastatin but not for calpain 3 in meat tenderization. In conclusion, CAST gene affected post-mortem activation time of calpain and drip loss.
authors: Department of Agri-food Protection and Valorization, Faculty of Agriculture, University of Bologna, via F.lli Rosselli 107, 42123 Reggio Emilia, Italy. greta.gandolfi2@unibo.it
authors: G
authors: G
authors: Gandolfi
authors: L
authors: L
authors: Pomponio
authors: P
authors: P
authors: Ertbjerg
authors: A H
authors: AH
authors: Karlsson
authors: L
authors: L
authors: Nanni Costa
authors: R
authors: R
authors: Lametsch
authors: V
authors: V
authors: Russo
authors: R
authors: R
authors: Davoli
mesh_terms: D000818:Animals; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism; D002154:Calpain / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D005838:Genotype; D008460:Meat / Q000032:analysis*; D009124:Muscle Proteins / Q000235:genetics* / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism*; D020641:Polymorphism, Single Nucleotide; D013552:Swine
chemical_list: D002135:Calcium-Binding Proteins; D009124:Muscle Proteins; C033668:calpastatin; D002154:Calpain