title: Comparison of the immunosuppressive effects of dexamethasone, flunixin meglumine and meloxicam on the in vitro response of calf peripheral blood mononuclear cells.
abstract: This study compared the immunosuppressive effects of dexamethasone (DEX), flunixin meglumine (FLU) and meloxicam (MEL) on the peripheral blood mononuclear cells (PBMCs) of seven healthy Holstein calves in vitro. DEX significantly inhibited lymphocyte proliferation and expression of interferon (IFN)-γ, interleukin (IL)-2 and IL-4 messenger RNA (mRNA) in comparison with FLU and MEL. FLU and MEL dose-dependently inhibited lymphocyte proliferation, but did not significantly reduce mRNA expression. Our in vitro study indicates that steroidal anti-inflammatory drugs (SAIDs) as well as nonsteroidal anti-inflammatory drugs (NSAIDs) have immunosuppressive effects on calf PBMCs. These findings are important for assessing the indications and complications of NSAIDs in calves.
authors: School of Veterinary Medicine, Kitasato University, Towada, Aomori 034-8628, Japan.
authors: Yousuke
authors: Y
authors: Maeda
authors: Rie
authors: R
authors: Tanaka
authors: Hiromichi
authors: H
authors: Ohtsuka
authors: Keiici
authors: K
authors: Matsuda
authors: Taishi
authors: T
authors: Tanabe
authors: Masaaki
authors: M
authors: Oikawa
mesh_terms: D000818:Animals; D002417:Cattle / Q000097:blood*; D049109:Cell Proliferation / Q000187:drug effects; D003002:Clonixin / Q000031:analogs & derivatives* / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology*; D005260:Female; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D007371:Interferon-gamma / Q000097:blood / Q000235:genetics / Q000276:immunology; D007376:Interleukin-2 / Q000097:blood / Q000235:genetics / Q000276:immunology; D015847:Interleukin-4 / Q000097:blood / Q000235:genetics / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000276:immunology*; D000077239:Meloxicam; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D013843:Thiazines / Q000494:pharmacology*; D013844:Thiazoles / Q000494:pharmacology*
chemical_list: D007166:Immunosuppressive Agents; D007376:Interleukin-2; D012333:RNA, Messenger; D013843:Thiazines; D013844:Thiazoles; D015847:Interleukin-4; D003907:Dexamethasone; D007371:Interferon-gamma; C014558:flunixin meglumine; D003002:Clonixin; D000077239:Meloxicam