title: Effects of miR-541 on neurite outgrowth during neuronal differentiation.
abstract: MicroRNA (miRNAs) are short non-coding RNA molecules that downregulate gene expression at post-transcriptional level. miRNAs are post-transcriptional regulators of gene expression important for neuron development and function. This report demonstrated that a putative and chemically synthesized miRNA rno-mir-541 played an important role in the neuron development. Differentiation of PC12 cells with nerve growth factor (NGF) is associated with neurite outgrowth, a process that involves upregulation of Synapsin I. We predicted, detected and assessed the expression levels of a number of possible miRNAs for synapsin I in rats and our outcomes showed that rno-mir-541 was associated with rat synapsin I expression. miR-541, a brain specific miRNA, plays an important role in repressing neurite extension in cultured PC12 neurons. The neurites of PC12 cells was shortened drasticly as a result of the overexpression of rno-mir-541. In contrast, the neurites of PC12 cell developed well after the knockdown of rno-mir-541 by RNA interference. Our study showed that rno-mir-541 played an important role in neuron-cell proliferation and neurite outgrowth through suppressing the expression of its target gene synapsin I. Furthermore, the introduction of NGF causes downregulation of miR-541, de-repression of its target, Synapsin-I and allows for neuritogenesis. Thus, miR-541 functions in neuronal precursors as an endogenous conditional component between NGF and Synapsin-I.
authors: Department of Regenerative Medicine, Tongji University School of Medicine, Shanghai, China. junzhang@tongji.edu.cn
authors: Jun
authors: J
authors: Zhang
authors: Jing
authors: J
authors: Zhang
authors: Li-hong
authors: LH
authors: Liu
authors: Yang
authors: Y
authors: Zhou
authors: Yi-ping
authors: YP
authors: Li
authors: Zhi-hua
authors: ZH
authors: Shao
authors: You-jun
authors: YJ
authors: Wu
authors: Meng-jie
authors: MJ
authors: Li
authors: Ying-ying
authors: YY
authors: Fan
authors: Hong-jun
authors: HJ
authors: Shi
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D015536:Down-Regulation; D018507:Gene Expression Regulation, Developmental; D055785:Gene Knockdown Techniques; D035683:MicroRNAs / Q000378:metabolism*; D020932:Nerve Growth Factor / Q000494:pharmacology; D016501:Neurites / Q000187:drug effects / Q000502:physiology*; D016716:PC12 Cells; D034622:RNA Interference; D051381:Rats; D016704:Synapsins / Q000378:metabolism*; D014162:Transfection; D015854:Up-Regulation
chemical_list: D035683:MicroRNAs; D016704:Synapsins; D020932:Nerve Growth Factor