title: Construction and use of a bottom-up HAC vector for transgene expression.
abstract: Recent technological advances have enabled visualization of the organization and dynamics of local -chromatin structures; however, the global mechanisms by which chromatin organization modulates gene regulation are poorly understood. We designed and constructed a human artificial chromosome (HAC) vector that allows regulation of transgene expression and delivery of a gene expression platform into many vertebrate cell lines. This technology for manipulating a transgene using a HAC vector could be used in applied biology.
authors: School of Medicine, Keio University, Tokyo, Japan. mikeno@fujita-hu.ac.jp
authors: Masashi
authors: M
authors: Ikeno
authors: Nobutaka
authors: N
authors: Suzuki
mesh_terms: D000818:Animals; D016466:CHO Cells; D002459:Cell Fusion; D022221:Chromosomes, Artificial, Mammalian / Q000235:genetics*; D006224:Cricetinae; D003412:Cricetulus; D015870:Gene Expression; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D006822:Hybrid Cells / Q000166:cytology / Q000378:metabolism; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D008938:Mitosis; D016254:Mutagenesis, Insertional; D010957:Plasmids / Q000235:genetics; D014162:Transfection; D019076:Transgenes / Q000235:genetics*