title: In vitro sensitivity of human metapneumovirus to type I interferons.
abstract: Human metapneumovirus (hMPV) has been recognized as an important respiratory pathogen. Due to its relatively recent discovery, only limited information is available on the relationship between hMPV and type I interferons (IFN). This study was designed to determine whether in vitro hMPV is sensitive to the antiviral activity of IFN-β, leukocyte IFN-α, and several IFN-α subtypes in a human Hep-2 cell line. The results showed that 50% inhibitory concentration values against hMPV for the various type I IFN preparations were significantly higher than those against the IFN-sensitive vesicular stomatitis virus, and some IFN-α subtypes appeared to be more active against hMPV than others, with IFN-α subtypes 5, 6, 8, and 10 being the most potent, and IFN-α2, 17, and 21 the least potent. The results show that hMPV grown in Hep-2 is partially resistant to the antiviral activity of type I IFNs. Additional studies are required to understand whether and to what extent the relatively low sensitivity of hMPV to IFNs influences the clinical outcomes of infected individuals.
authors: Department of Molecular Medicine, Laboratory of Virology, Sapienza University of Rome, Rome, Italy.
authors: Carolina
authors: C
authors: Scagnolari
authors: Simona
authors: S
authors: Trombetti
authors: Carla
authors: C
authors: Selvaggi
authors: Teresa
authors: T
authors: Carbone
authors: Katia
authors: K
authors: Monteleone
authors: Lucia
authors: L
authors: Spano
authors: Paola
authors: P
authors: Di Marco
authors: Alessandra
authors: A
authors: Pierangeli
authors: Fabrizio
authors: F
authors: Maggi
authors: Elisabetta
authors: E
authors: Riva
authors: Guido
authors: G
authors: Antonelli
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D022781:Hepatocytes / Q000821:virology; D006801:Humans; D020128:Inhibitory Concentration 50; D007370:Interferon Type I / Q000494:pharmacology*; D016898:Interferon-alpha / Q000494:pharmacology; D029121:Metapneumovirus / Q000187:drug effects*; D008826:Microbial Sensitivity Tests
chemical_list: D000998:Antiviral Agents; D007370:Interferon Type I; D016898:Interferon-alpha