title: A recombinant DNA vaccine encoding C. andersoni oocyst wall protein induces immunity against experimental C. parvum infection.
abstract: Cryptosporidium andersoni parasited in the abomasum has been demonstrated as a cause of reduction of milk production in dairy cow. In this study, a novel chimeric DNA vaccine pVAX1-AB was constructed and the efficacy against Cryptosporidium parvum was determined. BALB/c mice were divided into 3 groups and immunized with DNA vaccine expressing the oocyst wall protein, AB protein of C. andersoni, the recombinant plasmid containing the AB gene, respectively. After inoculation of 1 × 10(6) oocysts of C. parvum, the humoral and cellular immune responses were detected. Experimental results showed that the recombinant plasmid can induce corresponding specific antibody response, simultaneously influenced cellular immune responses, and provided greater protection rate (48.6%) than the other groups. These results indicated that chimeric DNA vaccine has a potential in Cryptosporidium vaccine development.
authors: College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun 130062, China.
authors: Jun
authors: J
authors: Zheng
authors: Wenzhi
authors: W
authors: Ren
authors: Qingshan
authors: Q
authors: Pan
authors: Qiuyue
authors: Q
authors: Wang
authors: I A Elfaki
authors: IA
authors: Elhag
authors: Jianhua
authors: J
authors: Li
authors: Mingying
authors: M
authors: Li
authors: Pengtao
authors: P
authors: Gong
authors: Yingli
authors: Y
authors: Liu
authors: Xichen
authors: X
authors: Zhang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D003457:Cryptosporidiosis / Q000517:prevention & control*; D003458:Cryptosporidium / Q000378:metabolism*; D005260:Female; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D033661:Oocysts / Q000378:metabolism*; D010957:Plasmids / Q000276:immunology; D015800:Protozoan Proteins / Q000276:immunology* / Q000378:metabolism; D016052:Protozoan Vaccines / Q000276:immunology*; D019444:Vaccines, DNA / Q000276:immunology
chemical_list: D000913:Antibodies, Protozoan; D015800:Protozoan Proteins; D016052:Protozoan Vaccines; D019444:Vaccines, DNA