title: Development of a nested PCR for environmental detection of the pathogenic free-living amoeba Balamuthia mandrillaris.
abstract: A DNA extraction and nested PCR method for detecting the pathogenic amoeba Balamuthia mandrillaris from the environment was developed. Sixteen of 17 Californian soil samples were positive compared with 0/44 from the United Kingdom. This approach will enable a greater understanding of B. mandrillaris ecology, geographic distribution, and public health risk.
authors: Department of Infection, Immunity & Inflammation, University of Leicester, Medical Sciences Building, PO Box 138, University Road, Leicester, LE1 9HN, United Kingdom.
authors: Arine F
authors: AF
authors: Ahmad
authors: Peter W
authors: PW
authors: Andrew
authors: Simon
authors: S
authors: Kilvington
mesh_terms: D056894:Amoebozoa / Q000235:genetics* / Q000302:isolation & purification*; D002140:California; D017931:DNA Primers / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D010274:Parasitology / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012988:Soil Microbiology; D006113:United Kingdom
chemical_list: D017931:DNA Primers