title: Nanoscale topography reduces fibroblast growth, focal adhesion size and migration-related gene expression on platinum surfaces.
abstract: Controlling cellular responses on biomaterial surfaces is crucial in biomedical applications such as tissue engineering and implantable prosthetics. Since cells encounter various nanoscale topographic features in their natural environment, it has been postulated that surface nanotopography may be an alternative route to fabricate biomaterials with a desirable cellular response. In this framework, we investigated the responses of primary human fibroblasts to platinum substrates with different levels of surface roughness at the nanoscale. The nanorough surfaces were fabricated by using the glancing angle deposition technique (GLAD). We found that levels of cellular responses depended on the surface roughness and the size of the nanoscale features. We showed that in response to nanotopography cells spread less and have an elongated morphology, displaying signs of actin cytoskeleton impairment and reduced formation of focal adhesion complexes. Although cell growth and adhesion were impaired on the nanorough substrates, cell viability was not affected by topography. To a minor extent our results also indicate that cell migration might be reduced on the nanorough surfaces, since a significantly lower gene expression of migration related genes were found on the roughest surfaces as compared to the flat reference. The results presented here demonstrate that surface nanotopography influences fibroblasts responses on platinum, which may be used to reduce cellular adhesion on platinum implant surfaces such as implantable neural electrodes.
authors: Laboratory for Stem Cell Research, Department of Health Science and Technology, Aalborg University. Frederik Bajers Vej 3B. DK-9220 Aalborg, Denmark. cpennisi@hst.aau.dk
authors: Cristian Pablo
authors: CP
authors: Pennisi
authors: Alireza
authors: A
authors: Dolatshahi-Pirouz
authors: Morten
authors: M
authors: Foss
authors: Jacques
authors: J
authors: Chevallier
authors: Trine
authors: T
authors: Fink
authors: Vladimir
authors: V
authors: Zachar
authors: Flemming
authors: F
authors: Besenbacher
authors: Ken
authors: K
authors: Yoshida
mesh_terms: D000199:Actins / Q000378:metabolism; D002465:Cell Movement / Q000187:drug effects* / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D022001:Focal Adhesions / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D018625:Microscopy, Atomic Force; D049329:Nanostructures / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods; D010984:Platinum / Q000737:chemistry / Q000494:pharmacology*; D013499:Surface Properties
chemical_list: D000199:Actins; D010984:Platinum