title: LipA, a tyrosine and lipid phosphatase involved in the virulence of Listeria monocytogenes.
abstract: Intracellular bacterial pathogens manipulate host cell functions by producing enzymes that stimulate or antagonize signal transduction. The Listeria monocytogenes genome contains a gene, lmo1800, encoding a protein with a conserved motif of conventional tyrosine phosphatases. Here, we report that the lmo1800-encoded protein LipA is secreted by Listeria and displays tyrosine as well as lipid phosphatase activity in vitro. Bacteria lacking LipA are severely attenuated in virulence in vivo, thus revealing a so-far-undescribed enzymatic activity involved in Listeria infection.
authors: Max F. Perutz Laboratories, Department of Microbiology, Immunobiology and Genetics, University of Vienna, Dr. Bohr-Gasse 9/4, 1030 Vienna, Austria.
authors: Renate
authors: R
authors: Kastner
authors: Olivier
authors: O
authors: Dussurget
authors: Cristel
authors: C
authors: Archambaud
authors: Elisabeth
authors: E
authors: Kernbauer
authors: Didier
authors: D
authors: Soulat
authors: Pascale
authors: P
authors: Cossart
authors: Thomas
authors: T
authors: Decker
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000502:physiology*; D015153:Blotting, Western; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D006462:Hemolytic Plaque Technique; D008089:Listeria monocytogenes / Q000201:enzymology / Q000472:pathogenicity*; D008088:Listeriosis / Q000382:microbiology*; D008264:Macrophages / Q000382:microbiology; D051379:Mice; D008810:Mice, Inbred C57BL; D017027:Protein Tyrosine Phosphatases / Q000502:physiology; D037521:Virulence Factors / Q000502:physiology*
chemical_list: D001426:Bacterial Proteins; C079999:LipA protein, Bacteria; D037521:Virulence Factors; D017027:Protein Tyrosine Phosphatases