title: Effect of preexisting immunity to adenovirus on transgene product-specific genital T cell responses on vaccination of mice with a homologous vector.
abstract: We evaluated changes in global and human immunodeficiency virus (HIV)-specific genital T cells after vaccination of female mice with a replication-defective adenovirus vector of human serotype 5 (AdHu5) expressing Gag protein of HIV-1, in the presence or absence of preexisting immunity to the vaccine carrier. Our data show that preexisting immunity causes a rapid and transient decrease of genital CD4(+) T cells without increasing the expression of chemokine (C-C motif) receptor 5. Furthermore, preexposure to AdHu5 affects long-term alterations in the magnitude and quality of vaccine-induced Gag-specific CD8(+) T cell responses. AdHu5-specific antibodies interfere with the induction of transgene product-specific CD8(+) T cell responses in systemic compartments, whereas some mechanism other than antibodies also seems to affect those that home to the genital tract.
authors: Immunology Program, Wistar Institute, 3601 Spruce St, Philadelphia, PA 19104-4268, USA.
authors: Larissa Herkenhoff
authors: LH
authors: Haut
authors: Sarah
authors: S
authors: Ratcliffe
authors: Aguinaldo Roberto
authors: AR
authors: Pinto
authors: Hildegund
authors: H
authors: Ertl
mesh_terms: D000256:Adenoviridae / Q000276:immunology*; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000502:physiology*; D018414:CD8-Positive T-Lymphocytes / Q000502:physiology*; D005260:Female; D015497:HIV-1 / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D014611:Vaccination; D014621:Vagina / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*; D054301:gag Gene Products, Human Immunodeficiency Virus / Q000276:immunology*
chemical_list: D014765:Viral Vaccines; D054301:gag Gene Products, Human Immunodeficiency Virus