title: Association of polymorphisms in glutamate-cysteine ligase catalytic subunit and microsomal triglyceride transfer protein genes with nonalcoholic fatty liver disease.
abstract: Genetic and environmental factors are important for the development of nonalcoholic fatty liver disease (NAFLD). The aim of the present study was to examine the single nucleotide polymorphism (SNP) -129C/T (rs17883901) in glutamate-cysteine ligase catalytic subunit (GCLC) and SNPs I128T (rs3816873) and Q95H (rs61733139) in microsomal triglyceride transfer protein (MTTP) in NAFLD. Eighty-three patients with a diagnosis of NAFLD and 93 healthy subjects were included in the study. Tetra amplification refractory mutation system-polymerase chain reaction was designed to detect the SNPs. There were no significant differences in the polymorphism of -129C/T (rs17883901) of the GCLC gene among NAFLD and control groups (p > 0.05). A significant difference was observed between NAFLD and control group regarding the SNP I128T (rs3816873) in the coding region of the MTTP gene (p < 0.05). The CT genotype increased susceptibility to NAFLD (OR: 2.467; 95% CI: 1.253-4.854; p = 0.008). No significant difference was found among the groups regarding the SNP in the coding region of MTTP gene Q95H (rs61733139). In conclusion, MTTP rs3816873 polymorphism might be a candidate to determine susceptibility to NAFLD. Larger studies are necessary to confirm these findings in various populations.
authors: Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran. mhd.hashemi@gmail.com
authors: Mohammad
authors: M
authors: Hashemi
authors: Hosnieh
authors: H
authors: Hoseini
authors: Parichehreh
authors: P
authors: Yaghmaei
authors: Abdolkarim
authors: A
authors: Moazeni-Roodi
authors: Ali
authors: A
authors: Bahari
authors: Norallah
authors: N
authors: Hashemzehi
authors: Sara
authors: S
authors: Shafieipour
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002352:Carrier Proteins / Q000235:genetics*; D016022:Case-Control Studies; D020134:Catalytic Domain / Q000235:genetics*; D005234:Fatty Liver / Q000201:enzymology* / Q000235:genetics*; D005260:Female; D005838:Genotype; D005721:Glutamate-Cysteine Ligase / Q000737:chemistry* / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D065626:Non-alcoholic Fatty Liver Disease; D020641:Polymorphism, Single Nucleotide; D055815:Young Adult
chemical_list: D002352:Carrier Proteins; C106092:microsomal triglyceride transfer protein; D005721:Glutamate-Cysteine Ligase