title: Viral small RNA cloning and sequencing.
abstract: At the current rate of technological progress, high-throughput sequencing of nucleic acids has become a commodity. These techniques are perfectly suitable for viral small RNAs sequencing and contribute to the understanding of many aspects of virus biology in the context of host-pathogen interaction. However, the generation of high quality data is still an issue and the preparation of small RNAs libraries that accurately reflect the viral siRNAs in the sample remains a challenge. In this chapter we describe how to clone and sequence libraries of viral small RNAs from infected insect samples (mosquito, drosophilidae, insect-derived cell lines).
authors: CNRS URA 3015, Institut Pasteur, Viruses and RNAi group, Paris, France.
authors: Valérie
authors: V
authors: Gausson
authors: Maria-Carla
authors: MC
authors: Saleh
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D019295:Computational Biology; D017931:DNA Primers / Q000235:genetics / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D007553:Isotope Labeling; D009841:Oligonucleotides / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D016133:Polymerase Chain Reaction; D034741:RNA, Small Interfering / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D048348:Reverse Transcription; D017423:Sequence Analysis, RNA / Q000379:methods*; D014780:Viruses / Q000235:genetics*
chemical_list: D017931:DNA Primers; D009841:Oligonucleotides; D034741:RNA, Small Interfering; D012367:RNA, Viral; C078388:endodeoxyribonuclease PmeI; D015252:Deoxyribonucleases, Type II Site-Specific