title: Application of a Fab fragment of monoclonal antibody specific to N-terminal pro-brain natriuretic peptide for the detection based on regeneration-free electrochemical immunosensor.
abstract: N-Terminal pro-brain natriuretic peptide (NT-proBNP) is an important marker for heart failure that reflects ventricular volume expansion, ventricular overload and the degree of cardiac injury. To establish a sensitive and simple detection method for it in serum, a regeneration-free immunosensor with novel Fab fragment monoclonal antibodies was prepared. The sensor detected NT-proBNP from 0.04 to 2.5 ng/ml with a limit of 0.03 ng/ml. The immunosensor is therefore a simple, cost-effective method to detect NT-proBNP and the proposed immunoassay system would enable other proteins to be detected and open new opportunities for protein diagnostics.
authors: Department of Clinical Biochemistry, Laboratory Sciences, Third Military Medical University, Chongqing, People's Republic of China.
authors: Weijing
authors: W
authors: Yi
authors: Wenbin
authors: W
authors: Liang
authors: Peng
authors: P
authors: Li
authors: Shuhui
authors: S
authors: Li
authors: Zhujun
authors: Z
authors: Zhang
authors: Mingzhen
authors: M
authors: Yang
authors: An
authors: A
authors: Chen
authors: Bo
authors: B
authors: Zhang
authors: Chuanmin
authors: C
authors: Hu
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000276:immunology*; D015415:Biomarkers / Q000032:analysis; D015374:Biosensing Techniques / Q000379:methods; D055664:Electrochemical Techniques / Q000379:methods*; D006333:Heart Failure / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry* / Q000276:immunology*; D007150:Immunohistochemistry; D058185:Magnetite Nanoparticles; D020097:Natriuretic Peptide, Brain / Q000097:blood / Q000276:immunology*; D010446:Peptide Fragments / Q000097:blood / Q000276:immunology*; D011994:Recombinant Proteins; D012044:Regression Analysis; D012680:Sensitivity and Specificity
chemical_list: D000911:Antibodies, Monoclonal; D015415:Biomarkers; D007140:Immunoglobulin Fab Fragments; D058185:Magnetite Nanoparticles; D010446:Peptide Fragments; D011994:Recombinant Proteins; C109794:pro-brain natriuretic peptide (1-76); D020097:Natriuretic Peptide, Brain