title: pmoA Primers for detection of anaerobic methanotrophs.
abstract: Published pmoA primers do not match the pmoA sequence of "Candidatus Methylomirabilis oxyfera," a bacterium that performs nitrite-dependent anaerobic methane oxidation. Therefore, new pmoA primers for the detection of "Ca. Methylomirabilis oxyfera"-like methanotrophs were developed and successfully tested on freshwater samples from different habitats. These primers expand existing molecular tools for the study of methanotrophs in the environment.
authors: Department of Microbiology, Institute for Water and Wetland Research, Radboud University Nijmegen, Heyendaalseweg 135, 6525 AJ Nijmegen, Netherlands.
authors: Francisca A
authors: FA
authors: Luesken
authors: Baoli
authors: B
authors: Zhu
authors: Theo A
authors: TA
authors: van Alen
authors: Margaret K
authors: MK
authors: Butler
authors: Marina Rodriguez
authors: MR
authors: Diaz
authors: Bongkeun
authors: B
authors: Song
authors: Huub J M
authors: HJ
authors: Op den Camp
authors: Mike S M
authors: MS
authors: Jetten
authors: Katharina F
authors: KF
authors: Ettwig
mesh_terms: D001421:Bacteria, Anaerobic / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics*; D001431:Bacteriological Techniques / Q000379:methods; D017931:DNA Primers / Q000235:genetics*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005618:Fresh Water / Q000382:microbiology; D008697:Methane / Q000378:metabolism*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; D004269:DNA, Bacterial; D008697:Methane