title: Antioxidant and ACE enhancing potential of Pankajakasthuri in fluoride toxicity: an in vitro study on mammalian lungs.
abstract: Fluoride toxicity occurs due to high concentrations of fluoride in water sources or anthropogenic causes. The aim of the present study was to investigate the effects of an Ayurvedic drug--Pankajakasthuri (PK)--in relation to fluoride-induced toxicity in mammalian lungs. The results indicated that sodium fluoride increased lipid peroxidation and decreased enzymatic and non-enzymatic antioxidants in a concentration-dependent manner in lungs. The antioxidant potential of the lungs was suppressed maximally at 10 ppm fluoride concentration and PK at all three dose levels (i.e., 100, 200 and 300 µl) decreased fluoride induced lipid peroxidation (p < 0.05) and increased the levels of total ascorbic acid, superoxide dismutase, catalase, reduced glutathione, glutathione peroxidase and FRAP values significantly (p < 0.05) in a dose-dependent manner. When PK was examined for its effects on angiotensin-converting enzyme (ACE) activity, in fluoride-induced toxicity, the ACE activity was found to increase (p < 0.0001) in lung homogenates with all three doses. This study indicates that PK, an Ayurvedic drug, improves mammalian lung function by increasing antioxidant potential and ACE activity under the conditions of fluoride toxicity.
authors: Laboratory for Animal Sciences, Department of Biosciences, Sardar Patel University, Vallabh Vidyanagar, Gujarat, India.
authors: Rupal A
authors: RA
authors: Vasant
authors: Mona C
authors: MC
authors: Khajuria
authors: A V R L
authors: AV
authors: Narasimhacharya
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000737:chemistry / Q000633:toxicity*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects*; D005458:Fluoride Poisoning / Q000188:drug therapy / Q000201:enzymology / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D006041:Goats; D015227:Lipid Peroxidation / Q000187:drug effects*; D008168:Lung / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D008297:Male; D008515:Medicine, Ayurvedic; D010084:Oxidation-Reduction / Q000187:drug effects; D010088:Oxidoreductases / Q000378:metabolism; D007703:Peptidyl-Dipeptidase A / Q000737:chemistry / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D028321:Plant Preparations / Q000737:chemistry; D012969:Sodium Fluoride / Q000037:antagonists & inhibitors* / Q000633:toxicity
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D000975:Antioxidants; D010936:Plant Extracts; D028321:Plant Preparations; D012969:Sodium Fluoride; D010088:Oxidoreductases; D007703:Peptidyl-Dipeptidase A; D005978:Glutathione; D001205:Ascorbic Acid