title: Morphologically abnormal oocytes not capable of fertilization despite repeated strategies.
abstract: OBJECTIVE
  To describe a case with repeated total fertilization failure due to oocyte defect.
  
  
  METHODS
  Case report.
  
  
  METHODS
  University hospital.
  
  
  METHODS
  A patient with repeated total fertilization failure.
  
  
  METHODS
  Assisted oocyte activation, intracytoplasmic sperm injection (ICSI) with calcium ionophore oocyte activation, donor semen, and donor oocytes.
  
  
  METHODS
  Fertilization, pregnancy, and live birth.
  
  
  RESULTS
  Five cycles of IVF/ICSI were performed. In the first IVF cycle, 19 oocytes failed to fertilize. In the second ICSI attempt, 12 oocytes were retrieved and were not fertilized. In the third and fourth natural cycles, donor semen was used for IVF and ICSI, respectively, yet the oocytes could not be fertilized even by assisted activation. Then donor oocytes were used in the ﬁfth cycle by ICSI. All five donated oocytes fertilized normally, and three embryos were transferred on day 3. Clinical pregnancy was confirmed, and a healthy girl weighing 3,200 g was delivered at 39 weeks of gestation by cesarean section.
  
  
  CONCLUSIONS
  In cases of repeated fertilization failure caused by oocyte defects, oocyte donation seems to be a good choice for patients who wish to become pregnant.
authors: Shandong Provincial Key Laboratory of Reproductive Medicine, Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, Jinan, People's Republic of China.
authors: Xiuye
authors: X
authors: Xing
authors: Han
authors: H
authors: Zhao
authors: Mei
authors: M
authors: Li
authors: Mei
authors: M
authors: Sun
authors: Yuan
authors: Y
authors: Li
authors: Zi-Jiang
authors: ZJ
authors: Chen
mesh_terms: D000328:Adult; D002585:Cesarean Section; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007317:Insemination, Artificial, Homologous; D050498:Live Birth; D008297:Male; D018587:Oocyte Donation; D009865:Oocytes / Q000473:pathology*; D010062:Ovulation Induction; D011247:Pregnancy; D027724:Reproductive Techniques, Assisted; D020554:Sperm Injections, Intracytoplasmic; D017211:Treatment Failure