title: Optimization of sulfated modification conditions of tremella polysaccharide and effects of modifiers on cellular infectivity of NDV.
abstract: Based on our previous research, sulfated modification conditions of Tremella polysaccharide (TPS), the chlorosulfonic acid to pyridine (CSA-Pry) ratio, reaction temperature and time, were optimized by L(9) (3(4)) orthogonal design taking the yield and degree of sulfation (DS) of modifiers as indexes. Two TPSs, TPS(tp) and TPS(70c), were modified under optimized conditions. The effects of two modifiers, sTPS(tp) and sTPS(70c), on cellular infectivity of NDV were determined by MTT method taking the non-modified TPS(tp), TPS(tc) and TPS(70c) as controls. The results showed that the optimized modification conditions were reaction temperature of 80°C, CSA-Pry ratio of 1:6 and reaction time of 1.5h. Five polysaccharides at proper concentrations could significantly inhibit the infectivity of NDV to CEF. The virus inhibitory rates of sTPS(tp) at 1.563 μg mL(-1) group were the highest and significantly higher than those of other three non-modified polysaccharide groups in three sample-adding modes. This indicated that sulfated modification could significantly improve the antiviral activity of TPS. sTPS(tp) possessed the best efficacy and would be as a component of antiviral polysaccharide drug.
authors: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors: Xiaona
authors: X
authors: Zhao
authors: Yuanliang
authors: Y
authors: Hu
authors: Deyun
authors: D
authors: Wang
authors: Liwei
authors: L
authors: Guo
authors: Shujuan
authors: S
authors: Yang
authors: Yunpeng
authors: Y
authors: Fan
authors: Bingkai
authors: B
authors: Zhao
authors: Yuanlei
authors: Y
authors: Wang
authors: Saifuding
authors: S
authors: Abula
mesh_terms: D000818:Animals; D001487:Basidiomycota / Q000737:chemistry*; D002642:Chick Embryo; D005347:Fibroblasts; D009522:Newcastle disease virus / Q000502:physiology*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D013047:Specific Pathogen-Free Organisms; D013431:Sulfates / Q000737:chemistry*; D013696:Temperature; D013778:Tetrazolium Salts; D013844:Thiazoles; D013997:Time Factors; D016662:Virus Integration / Q000502:physiology*
chemical_list: D011134:Polysaccharides; D013431:Sulfates; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue