title: Electron detachment dissociation for top-down mass spectrometry of acidic proteins.
abstract: Electron detachment dissociation (EDD) is an emerging mass spectrometry (MS) technique for the primary structure analysis of peptides, carbohydrates, and oligonucleotides. Herein, we explore the potential of EDD for sequencing of proteins of up to 147 amino acid residues by using top-down MS. Sequence coverage ranged from 72% for Melittin, which lacks carboxylic acid functionalities, to 19% for an acidic 147-residue protein, to 12% for Ferredoxin, which showed unusual backbone fragmentation next to cysteine residues. A limiting factor for protein sequencing by EDD is the facile loss of small molecules from amino acid side chains, in particular CO(2). Based on the types of fragments observed and fragmentation patterns found, we propose detailed mechanisms for protein backbone cleavage and side chain dissociation in EDD. The insights from this study should further the development of EDD for top-down MS of acidic proteins.
authors: Institute of Organic Chemistry and Center for Molecular Biosciences Innsbruck (CMBI), University of Innsbruck, Innrain 52a, 6020 Innsbruck, Austria.
authors: Barbara
authors: B
authors: Ganisl
authors: Taras
authors: T
authors: Valovka
authors: Markus
authors: M
authors: Hartl
authors: Monika
authors: M
authors: Taucher
authors: Klaus
authors: K
authors: Bister
authors: Kathrin
authors: K
authors: Breuker
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D004583:Electrons; D005288:Ferredoxins / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D013058:Mass Spectrometry / Q000379:methods*; D008555:Melitten / Q000737:chemistry; D008958:Models, Molecular; D015394:Molecular Structure; D011506:Proteins / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D005288:Ferredoxins; D011506:Proteins; D008555:Melitten