title: Rapid molecular strategy for orbivirus detection and characterization.
abstract: Orbiviruses infect a wide range of hosts, including humans. The ability to detect them has been hampered by their diversity. Here we present a simple consensus reverse transcription (RT)-PCR method targeting the polymerase gene for orbivirus recognition and characterization. Phylogenetic assignment is achieved by automated Web-based sequence analysis of amplification products.
authors: Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, New York 10032, USA. gp2050@columbia.edu
authors: Gustavo
authors: G
authors: Palacios
authors: Chris
authors: C
authors: Cowled
authors: Ana V
authors: AV
authors: Bussetti
authors: Nazir
authors: N
authors: Savji
authors: Richard
authors: R
authors: Weir
authors: Ivan
authors: I
authors: Wick
authors: Amelia
authors: A
authors: Travassos da Rosa
authors: Charles H
authors: CH
authors: Calisher
authors: Robert B
authors: RB
authors: Tesh
authors: David
authors: D
authors: Boyle
authors: W Ian
authors: WI
authors: Lipkin
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D006801:Humans; D020407:Internet; D008969:Molecular Sequence Data; D018163:Orbivirus / Q000302:isolation & purification*; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D012088:Reoviridae Infections / Q000175:diagnosis* / Q000662:veterinary*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014764:Viral Proteins / Q000235:genetics; D014773:Virology / Q000379:methods*
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins; D012324:RNA-Dependent RNA Polymerase