title: [Effects of different factors on transformed hairy root in Gynostemm apentaphyllum].
abstract: OBJECTIVE
  To induce hairy roots of Gynostemm apentaphyllum by Agrobacterium rhizogenes strains.
  
  
  METHODS
  Hairy roots were induced by the co-culture method of explants and Agrobacterium rhizogenes strains. Effects of different Agrobacterium rhizogenes strains, explants, pre(co)-culture time, Bacterial concentration, infecting time, As concentration and antibiotic medium on the transformation frequency were studied.
  
  
  RESULTS
  The highest induction frequency was obtained form leaf 2 days co-cultivation, which were induced by Agrobacterium rhizogenes OD 600 0. 8 for 10 min, 100 micromol/L As and MS + 300 mg/L Cab.
  
  
  CONCLUSIONS
  Hairy roots were induced by co-cultivation and the optimum induced condition were determined.
authors: College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China. hefengfa@126.com
authors: Feng-Fa
authors: FF
authors: He
authors: Tian
authors: T
authors: Tian
authors: Gui-Xue
authors: GX
authors: Wang
authors: Qian
authors: Q
authors: Tang
authors: Jian-Ji
authors: JJ
authors: Zhang
mesh_terms: D002228:Carbenicillin / Q000378:metabolism; D002439:Cefotaxime / Q000378:metabolism; D018920:Coculture Techniques; D003470:Culture Media; D046508:Culture Techniques / Q000379:methods; D029774:Gynostemma / Q000235:genetics / Q000254:growth & development* / Q000382:microbiology*; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development; D018517:Plant Roots / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D018547:Plant Stems / Q000235:genetics / Q000254:growth & development; D012231:Rhizobium / Q000235:genetics / Q000502:physiology; D014170:Transformation, Genetic
chemical_list: D003470:Culture Media; D002228:Carbenicillin; D002439:Cefotaxime