title: Fast and long term lipid droplet tracking with CARS microscopy.
abstract: Photobleaching of organic fluorophores commonly used in fluorescence microscopy puts a limit to the number of images which can be acquired. Label-free imaging techniques therefore offer advantages both for rapid image acquisition and for long-term observations. CARS microscopy is a label-free imaging technique offering molecule specific contrast. Here we demonstrate that CARS microscopy allows video-rate tracking of intracellular transport of lipid droplets, but also continuous long-term observation of cells over several hours.
authors: Departement Chemie and Center for Applied Photonics, Universität Konstanz, 78457 Konstanz, Germany.
authors: Christian
authors: C
authors: Jüngst
authors: Martin J
authors: MJ
authors: Winterhalder
authors: Andreas
authors: A
authors: Zumbusch
mesh_terms: D004867:Equipment Design; D006367:HeLa Cells; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008055:Lipids / Q000737:chemistry*; D008853:Microscopy / Q000379:methods*; D018613:Microscopy, Confocal / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods*; D018715:Microscopy, Video / Q000379:methods; D008870:Microtubules / Q000378:metabolism; D008938:Mitosis; D055095:Optics and Photonics; D012984:Software; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D008055:Lipids