title: A 2,7-carbazole-based dicationic salt for fluorescence detection of nucleic acids and two-photon fluorescence imaging of RNA in nucleoli and cytoplasm.
abstract: A new carbazole-derived dicationic compound, namely 2,7-bis(1-hydroxyethyl-4-vinylpyridinium iodine)-N-ethylcarbazole (2,7-9E-BHVC), with a large two-photon action absorption cross section in nucleic acids has been obtained. Moreover, it possesses the potential of imaging RNA in nucleoli and cytoplasm in two-photon fluorescence microscopy and exhibits good counterstain compatibility with the commercial fluorescent nucleic dye DAPI.
authors: State Key Laboratory of Crystal Materials, Shandong University, Jinan, P R China.
authors: Xin
authors: X
authors: Liu
authors: Yuming
authors: Y
authors: Sun
authors: Yuanhong
authors: Y
authors: Zhang
authors: Fang
authors: F
authors: Miao
authors: Guancong
authors: G
authors: Wang
authors: Hongshi
authors: H
authors: Zhao
authors: Xiaoqiang
authors: X
authors: Yu
authors: Hong
authors: H
authors: Liu
authors: Wai-Yeung
authors: WY
authors: Wong
mesh_terms: D002227:Carbazoles / Q000138:chemical synthesis / Q000737:chemistry*; D002466:Cell Nucleolus / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods*; D017785:Photons; D011726:Pyridinium Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D012313:RNA / Q000737:chemistry / Q000378:metabolism*; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: C560403:2,7-bis(1-hydroxyethyl-4-vinylpyridinium iodine)-N-ethylcarbazole; D002227:Carbazoles; D005456:Fluorescent Dyes; D011726:Pyridinium Compounds; C041514:carbazole; D012313:RNA