title: [Breathing activity of suspension culture of cells of Polyscias filicifolia Bailey, Stephania glabra (Roxb.) Miers, and Dioscorea deltoidea Wall].
abstract: Peculiarities of breathing of cultures of cells producing biologically active compounds (isoprenoids and alkaloids) were investigated in order to optimize productivity of culture growth and biosynthesis. It had been revealed that studied cultures of cells of Dioscorea deltoidea Wall (producer of furistanol glycosides), Stephania glabra (Roxb.) Miers (producer of stepharin alkaloid) and Polyscias filicifolia Bailey (complex of biologically active agents) differ both in joint breathing activity and in ratio between cytochrome and cyanide-resistant breathing, while changes of rate of total oxygen consumption and activity of alternative oxidase during growth were found to be individual for every investigated culture. Maximum rate of oxygen consumption for cells of D. deltoidea and S. glabra was marked in the period preceding active synthesis of secondary metabolites (lag phase for D. deltoidea and exponential phase for S. glabra). The revealed trends can be used for further monitoring and regulation of growth and biosynthesis of secondary metabolites in producing cell cultures during deep cultivation.
authors: M V
authors: MV
authors: Titova
authors: E A
authors: EA
authors: Berkovich
authors: O V
authors: OV
authors: Reshetniak
authors: I E
authors: IE
authors: Kulichenko
authors: A V
authors: AV
authors: Oreshnikov
authors: A M
authors: AM
authors: Nosov
mesh_terms: D000470:Alkaloids / Q000032:analysis / Q000096:biosynthesis; D027481:Araliaceae / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D018533:Biomass; D018929:Cell Culture Techniques; D002453:Cell Cycle; D049109:Cell Proliferation; D002470:Cell Survival; D027742:Dioscorea / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D006027:Glycosides / Q000096:biosynthesis; D010085:Oxidative Phosphorylation; D010101:Oxygen Consumption / Q000502:physiology*; D013045:Species Specificity; D031607:Stephania / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D013535:Suspensions; D013729:Terpenes / Q000378:metabolism
chemical_list: D000470:Alkaloids; D006027:Glycosides; D013535:Suspensions; D013729:Terpenes