title: [Enzyme immunoassay for the determination of hexestrol in meat].
abstract: An indirect competitive enzyme-linked immunosorbent assay (ELISA) of hexestrol (HES), an antibiotic forbidden for use in livestock farming, has been developed. Conditions of ELISA have been optimized by varying the concentrations of the coating conjugate (HES-ovalbumin), anti-HES antiserum, casein, and Tween 20. In the absence of Tween 20 in the reaction mixture, the detection limit (IC10) equaled 0.01 ng/ml, IC50 equaled 0.17 ng/ml, and the working range (IC20-IC80) equaled 0.03-0.86 ng/ml, while, in the presence of 0.05% Tween 20, these values equaled 0.05 ng/ml, 2.9 ng/ml, and 0.26-32.0 ng/ml, respectively. Standard deviation of the analysis results did not exceed 5.4%. If ELISA was performed in the absence of detergents, the recovery value upon HES determination in spiked beef samples ranged from 74 to 147%.
authors: M M
authors: MM
authors: Vdovenko
authors: C F
authors: CF
authors: Peng
authors: C L
authors: CL
authors: Xu
authors: E S
authors: ES
authors: Vylegzhanina
authors: A A
authors: AA
authors: Komarov
authors: I Iu
authors: IIu
authors: Sakharov
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D000906:Antibodies / Q000378:metabolism; D001667:Binding, Competitive; D002364:Caseins / Q000737:chemistry; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005506:Food Contamination / Q000032:analysis; D006241:Haptens / Q000737:chemistry / Q000378:metabolism; D006589:Hexestrol / Q000032:analysis*; D007106:Immune Sera / Q000737:chemistry; D020128:Inhibitory Concentration 50; D057230:Limit of Detection; D008460:Meat / Q000032:analysis; D010047:Ovalbumin / Q000737:chemistry / Q000378:metabolism; D011136:Polysorbates / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D000906:Antibodies; D002364:Caseins; D006241:Haptens; D007106:Immune Sera; D011136:Polysorbates; D006589:Hexestrol; D010047:Ovalbumin