title: Overexpression of Shadoo protein in transgenic mice does not impact the pathogenesis of scrapie.
abstract: Shadoo is a glycoprotein expressed in the adult brain that is an interacting protein of prion protein; however, its function remains to be determined. To elucidate its role in prion pathogenesis, we generated transgenic mice overexpressing wild-type (wt) Shadoo driven by the murine PrP promoter. Expression of the murine Sprn transgene significantly increased brain Shadoo protein levels in all three mouse lines generated. Following infection with mouse-adapted scrapie strain 22L, all transgenic mice tested exhibited characteristics of scrapie disease. Importantly, there was no correlation between the expression level or incubation time of Shadoo with disease phenotype. We therefore conclude that Shadoo has little or no influence on the outcome of transmissible spongiform encephalopathy (TSE) disease in transgenic mice.
authors: Department of Animal Biotechnology, College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China.
authors: Haiying
authors: H
authors: Wang
authors: Jiayu
authors: J
authors: Wan
authors: Wei
authors: W
authors: Wang
authors: Dongxu
authors: D
authors: Wang
authors: Sha
authors: S
authors: Li
authors: Peng
authors: P
authors: Liao
authors: Zhuo
authors: Z
authors: Hao
authors: Songbo
authors: S
authors: Wu
authors: Jing
authors: J
authors: Xu
authors: Nan
authors: N
authors: Li
authors: Hongsheng
authors: H
authors: Ouyang
authors: Hongwei
authors: H
authors: Gao
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D058851:GPI-Linked Proteins; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D010641:Phenotype; D018620:PrPSc Proteins / Q000235:genetics / Q000378:metabolism; D012608:Scrapie / Q000235:genetics / Q000473:pathology*
chemical_list: D058851:GPI-Linked Proteins; D005904:Glial Fibrillary Acidic Protein; D009419:Nerve Tissue Proteins; D018620:PrPSc Proteins; C523856:Sprn protein, mouse