title: Detailed kinetic model describing new oligosaccharides synthesis using different β-galactosidases.
abstract: The production of prebiotic galactooligosaccharides (GOS) from lactose has been widely studied whereas the synthesis of new prebiotic oligosaccharides with improved properties as those derived from lactulose is receiving an increasing interest. Understanding the mechanism of enzymatic oligosaccharides synthesis from lactulose would help to improve the quality of the products in a rational way as well as to increase the production efficiency by optimally selecting the operating conditions. A detailed kinetic model describing the enzymatic transgalactosylation reaction during lactulose hydrolysis is presented here for the first time. The model was calibrated with the experimental data obtained in batch assays with two different β-galactosidases at various temperatures and concentrations of substrate. A complete system identification loop, including model selection, robust estimation of the parameters by means of a global optimization method and computation of confidence intervals was performed. The kinetic model showed a good agreement between experimental data and predictions for lactulose conversion and provided important insights into the mechanism of formation of new oligosaccharides with potential prebiotic properties.
authors: (Bio)Process Engineering Group, Instituto de Investigaciones Marinas (CSIC), Eduardo Cabello, 6, 36208 Vigo, Spain. mrodriguez@iim.csic.es
authors: Maria
authors: M
authors: Rodriguez-Fernandez
authors: Alejandra
authors: A
authors: Cardelle-Cobas
authors: Mar
authors: M
authors: Villamiel
authors: Julio R
authors: JR
authors: Banga
mesh_terms: D001230:Aspergillus / Q000201:enzymology; D001426:Bacterial Proteins / Q000378:metabolism; D005690:Galactose / Q000378:metabolism; D006868:Hydrolysis; D007700:Kinetics; D007716:Kluyveromyces / Q000201:enzymology; D007792:Lactulose / Q000378:metabolism; D008954:Models, Biological; D009844:Oligosaccharides / Q000378:metabolism*; D056692:Prebiotics; D015203:Reproducibility of Results; D013696:Temperature; D001616:beta-Galactosidase / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D009844:Oligosaccharides; D056692:Prebiotics; D007792:Lactulose; D001616:beta-Galactosidase; D005690:Galactose