title: A new biosynthetic tracer for the inline measurement of virus retention in membrane processes: part I--Synthesis protocol.
abstract: In this study, a new biosynthetic tracer was developed to characterize the virus retention dynamics of membrane systems. This new tracer is a modified bacteriophage obtained by the grafting of enzymatic probes to an MS2 bacteriophage, one of the smallest non-pathogenic bacteria viruses, with an average diameter of about 30 nm. A protocol for the synthesis and purification of this new tracer was developed in this work. The production of this biosynthetic tracer was first qualitatively shown by a chromatographic characterization and an enzymatic test. The average number of probes grafted per phage was then quantified for three batches of tracers made from the same native phage suspension and the same batch of enzymatic probes. This quantification demonstrated the reproducibility of the synthesis protocol developed.
authors: Université de Toulouse, INSA, LISBP, 135 Avenue de Rangueil, INRA, UMR792 Ingénierie des Systèmes Biologiques et des Procédés, CNRS, F-31400 Toulouse, France. Laurence.Soussan@hotmail.fr
authors: L
authors: L
authors: Soussan
authors: C
authors: C
authors: Guigui
authors: S
authors: S
authors: Alfenore
authors: S
authors: S
authors: Mathe
authors: C
authors: C
authors: Cabassud
mesh_terms: D001360:Avidin / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods; D001710:Biotin / Q000737:chemistry; D002850:Chromatography, Gel / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006735:Horseradish Peroxidase / Q000378:metabolism; D017909:Levivirus / Q000302:isolation & purification*
chemical_list: C093483:neutravidin; D001360:Avidin; D001710:Biotin; D006735:Horseradish Peroxidase