title: Association of PON1 and APOA5 gene polymorphisms in a cohort of Indian patients having coronary artery disease with and without type 2 diabetes.
abstract: Type 2 diabetes mellitus (T2DM) is a major cause of coronary artery disease (CAD) and is responsible for a great deal of morbidity and mortality in Asian Indians. Several gene polymorphisms have been associated with CAD and T2DM in different ethnic groups. This study will give an insight about the association of two selected candidate gene polymorphisms; paraoxonase1 (PON1) Q192R and apolipoprotein A5 (APOA5) -1131T>C were assessed in a cohort of South Indian patients having CAD with and without T2DM. Polymerase chain reaction-based genotyping of PON1 Q192R (rs662) and APOA5-1131T>C (rs662799) polymorphism was carried out in 520 individuals, including 250 CAD patients (160 with T2DM and 90 without T2DM), 150 T2DM patients with no identified CAD, and 120 normal healthy sex- and age-matched individuals as controls. The PON1 192RR genotype and R allele frequency were elevated in both CAD and T2DM patients when compared with controls; however, only CAD patients with T2DM showed a statistical significance (p=0.023; OR=1.49; 95% CI: 1.04-2.12) when compared with controls. The APOA5-1131CC genotype and C allele also showed a significant association between the CAD+T2DM patients when compared with CAD without T2DM and healthy controls (p=0.012; OR=1.71; 95% CI: 1.0-2.67). An additive interaction between the PON1 RR and APOA5 TC genotypes was identified between the T2DM and CAD patients (p=0.028 and 0.0382, respectively). PON1 and APOA5 polymorphisms may serve as biomarkers in the South Indian population to identify T2DM patients who are at risk of developing CAD.
authors: Genome Research Group, Centre for Cellular and Molecular Biology, Council for Scientific and Industrial Research, Hyderabad, India. seema@ccmb.res.in
authors: Seema
authors: S
authors: Bhaskar
authors: Mala
authors: M
authors: Ganesan
authors: Giriraj Ratan
authors: GR
authors: Chandak
authors: Radha
authors: R
authors: Mani
authors: Mohammed M
authors: MM
authors: Idris
authors: Nasaruddin
authors: N
authors: Khaja
authors: Suryaprakash
authors: S
authors: Gulla
authors: Uday
authors: U
authors: Kumar
authors: Sireesha
authors: S
authors: Movva
authors: Kiran K
authors: KK
authors: Vattam
authors: Kavita
authors: K
authors: Eppa
authors: Qurratulain
authors: Q
authors: Hasan
authors: Umamaheshwara Reddy
authors: UR
authors: Pulakurthy
mesh_terms: D000072040:Apolipoprotein A-V; D001054:Apolipoproteins A / Q000235:genetics*; D043303:Aryldialkylphosphatase / Q000235:genetics*; D016022:Case-Control Studies; D003324:Coronary Artery Disease / Q000453:epidemiology / Q000209:etiology / Q000235:genetics*; D003924:Diabetes Mellitus, Type 2 / Q000150:complications* / Q000453:epidemiology / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D007194:India / Q000453:epidemiology; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D044465:White People / Q000235:genetics*
chemical_list: C437704:APOA5 protein, human; D000072040:Apolipoprotein A-V; D001054:Apolipoproteins A; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human