title: Strong expression of CD133 is associated with increased cholangiocarcinoma progression.
abstract: OBJECTIVE
  To determine the role of CD133 in cholangiocarcinoma progression.
  
  
  METHODS
  CD133 protein expression was evaluated by immunohistochemistry in 34 cholangiocarcinoma specimens. In addition, proliferation, chemoresistance and invasive properties of CD133-enriched (CD133(+)) and CD133-depleted (CD133(-)) RMCCA1 cholangiocarcinoma cells were studied and compared.
  
  
  RESULTS
  Strong CD133 expression was observed in 67.6% (23/34) of the cholangiocarcinoma specimens. Strong expression of CD133 was significantly associated with nodal metastasis (P = 0.009) and positive surgical margin status (P = 0.011). In the in vitro study, both the CD133(+) and CD133(-) cells had similar proliferation abilities and resistance to chemotherapeutic drugs. However, the CD133(+) cells had a higher invasive ability compared with CD133(-) cells.
  
  
  CONCLUSIONS
  CD133+ cells play an important role in the invasiveness of cholangiocarcinoma. Targeting of the CD133+ cells may be a useful approach to improve treatment against cholangiocarcinoma.
authors: Department of Surgery, Rajavithi Hospital, Bangkok, 10400, Thailand. kawin.leelawat@gmail.com
authors: Kawin
authors: K
authors: Leelawat
authors: Taweesak
authors: T
authors: Thongtawee
authors: Siriluck
authors: S
authors: Narong
authors: Somboon
authors: S
authors: Subwongcharoen
authors: Sa-ad
authors: SA
authors: Treepongkaruna
mesh_terms: D000071916:AC133 Antigen; D015703:Antigens, CD / Q000276:immunology*; D001650:Bile Duct Neoplasms / Q000276:immunology* / Q000473:pathology*; D001653:Bile Ducts, Intrahepatic / Q000473:pathology*; D049109:Cell Proliferation; D018281:Cholangiocarcinoma / Q000276:immunology* / Q000473:pathology*; D018450:Disease Progression; D019008:Drug Resistance, Neoplasm; D006023:Glycoproteins / Q000276:immunology*; D006801:Humans; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D010455:Peptides / Q000276:immunology*; D015996:Survival Rate
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides