title: Intrasporangium chromatireducens sp. nov., a chromate-reducing actinobacterium isolated from manganese mining soil, and emended description of the genus Intrasporangium.
abstract: A gram-positive, aerobic actinobacterium with high chromate [Cr(VI)]-reducing ability, designated strain Q5-1(T), was isolated from manganese mining soil in Hunan Province, central-south China. The organism formed branching hyphae and contained ll-diaminopimelic acid in the cell-wall peptidoglycan. The polar lipid profile was characterized by diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside and an unidentified phospholipid. The major fatty acids were iso-C(14 : 0), iso-C(15 : 0), anteiso-C(15 : 0) and iso-C(16 : 0), and the predominant menaquinone was MK-8(H(4)). The genomic DNA G+C content was 71.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain Q5-1(T) was closely related to Intrasporangium calvum DSM 43043(T) ( = NRRL B-3866(T)) and Humihabitans oryzae KV-657(T) ( = NRRL B-24470(T)) with similarities of 96.6 and 96.4 %, respectively. Comparison of phenotypic, biochemical and chemotaxonomic characters of strain Q5-1(T) and phylogenetically related strains revealed that the isolate represents a novel species of the genus Intrasporangium, for which the name Intrasporangium chromatireducens sp. nov. is proposed. The type strain is Q5-1(T) ( = KCTC 19811(T) = CCTCC AA 2010019(T) = CGMCC 1.10750(T) = NRRL B-59521(T)). An emended description of the genus Intrasporangium is also proposed.
authors: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.
authors: Hongliang
authors: H
authors: Liu
authors: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.
authors: Hui
authors: H
authors: Wang
authors: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.
authors: Gejiao
authors: G
authors: Wang
mesh_terms: D000192:Actinomycetales / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques; D002681:China; D002840:Chromates / Q000378:metabolism*; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D008055:Lipids / Q000032:analysis; D008345:Manganese; D008906:Mining; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013045:Species Specificity; D024482:Vitamin K 2 / Q000032:analysis
chemical_list: D002840:Chromates; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D008055:Lipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D008345:Manganese