title: Actions of a picomolar short-acting S1P₁ agonist in S1P₁-eGFP knock-in mice.
abstract: Sphingosine 1-phosphate receptor 1 (S1P(1)) is critical for lymphocyte recirculation and is a clinical target for treatment of multiple sclerosis. By generating a short-duration S1P(1) agonist and mice in which fluorescently tagged S1P(1) replaces wild-type receptor, we elucidate physiological and agonist-perturbed changes in expression of S1P(1) at a subcellular level in vivo. We demonstrate differential downregulation of S1P(1) on lymphocytes and endothelia after agonist treatment.
authors: Department of Chemical Physiology, The Scripps Research Institute, La Jolla, California, USA.
authors: Stuart M
authors: SM
authors: Cahalan
authors: Pedro J
authors: PJ
authors: Gonzalez-Cabrera
authors: Gor
authors: G
authors: Sarkisyan
authors: Nhan
authors: N
authors: Nguyen
authors: Marie-Therese
authors: MT
authors: Schaeffer
authors: Liming
authors: L
authors: Huang
authors: Adam
authors: A
authors: Yeager
authors: Bryan
authors: B
authors: Clemons
authors: Fiona
authors: F
authors: Scott
authors: Hugh
authors: H
authors: Rosen
mesh_terms: D000818:Animals; D015536:Down-Regulation / Q000187:drug effects; D004727:Endothelium / Q000187:drug effects / Q000378:metabolism; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism; D055879:Gene Knock-In Techniques; D049452:Green Fluorescent Proteins / Q000737:chemistry* / Q000378:metabolism; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D009103:Multiple Sclerosis / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D049349:Receptors, Lysosphingolipid / Q000819:agonists* / Q000378:metabolism / Q000627:therapeutic use*; D013997:Time Factors
chemical_list: D005456:Fluorescent Dyes; D049349:Receptors, Lysosphingolipid; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins