title: Development of monoclonal antibodies to highly pathogenic avian influenza H5N1 virus and their application to diagnostics, prophylaxis, and therapy.
abstract: A panel of 17 monoclonal antibodies (MAbs) against highly pathogenic avian influenza virus (HPAIV) A/Duck/Novosibirsk/56/05 A/H5N1 (subclade 2.2) isolated in Russian Federation was developed. Immunoblot analysis showed that 12 MAbs were specific for the hemagglutinin (HA) and 5 MAbs for nucleoprotein (NP). All anti-HA MAbs were reactive in ELISA and immunofluorescence (IF) test and 10 of them were reactive in hemagglutination-inhibition (HI) and neutralization tests. Quantitative competitive ELISA revealed that anti-HA MAbs recognized at least 4 non-overlapping antigenic determinants and anti-NP MAbs recognized at least 3 non-overlapping antigenic determinants. Four sandwich ELISA procedures were developed using the obtained MAbs. These procedures are useful for 1) identification of avian, human, and swine influenza A viruses, 2) differentiation of avian influenza virus (AIV) from human and swine influenza viruses, 3) differentiation of AIV H5 from other AIV subtypes, and 4) differentiation between 2.2 and 2.3.2 subclades of H5N1 influenza viruses. Prophylactic and therapeutic efficacy of anti-HA MAbs with high neutralization activity was tested in BALB/c mice. A complete protection was achieved by single injection of MAbs (20 mg/kg) 24 hrs before challenge with 10 LD50 of HPAIV H5N1. Therapeutic efficacy was 90% that was similar to those of Rimantadine and Tamiflu.
authors: Russian Academy of Medical Sciences, Moscow, Russian Federation. ol.mas@mail.ru
authors: O V
authors: OV
authors: Masalova
authors: R R
authors: RR
authors: Klimova
authors: E V
authors: EV
authors: Chichev
authors: I T
authors: IT
authors: Fediakina
authors: S Y
authors: SY
authors: Loginova
authors: S V
authors: SV
authors: Borisevich
authors: V P
authors: VP
authors: Bondarev
authors: P G
authors: PG
authors: Deryabin
authors: D K
authors: DK
authors: Lvov
authors: A A
authors: AA
authors: Kushch
mesh_terms: D000818:Animals; D019072:Antibiotic Prophylaxis / Q000379:methods; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology* / Q000494:pharmacology; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology; D001717:Birds / Q000276:immunology* / Q000821:virology*; D002642:Chick Embryo; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D000939:Epitopes / Q000276:immunology; D006385:Hemagglutination Inhibition Tests / Q000379:methods; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000276:immunology; D015151:Immunoblotting / Q000379:methods; D053124:Influenza A Virus, H5N1 Subtype / Q000276:immunology*; D009980:Influenza A virus / Q000276:immunology*; D005585:Influenza in Birds / Q000175:diagnosis / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009976:Orthomyxoviridae Infections / Q000517:prevention & control; D013552:Swine
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes; D019267:Hemagglutinin Glycoproteins, Influenza Virus