title: Determination of phytic acid and inositolphosphates in barley.
abstract: Phytic acid (PA) and lower inositolphosphates (InsP(n) ) is the main storage form of phosphorus in grains or seeds. The content of PA and InsP(n) in different varieties of barley was analyzed by capillary isotachophoresis and online-coupled capillary isotachophoresis with CZE. The electrolytes (in demineralized water) for the isotachophoretic analysis consisted of 10 mM HCl, 14 mM glycylglycine, and 0.1% 2-hydroxyethylcellulose (leading) and 10 mM citric acid (terminating). The optimized electrolytes for the online coupling isotachophoresis with zone electrophoresis analysis were mixtures of 5 mM HCl, 7 mM glycylglycine, and 0.1% 2-hydroxyethylcellulose (leading), 20 mM citric acid, 10 mM glycylglycine, and 0.1% 2-hydroxyethylcellulose (background) and 10 mM citric acid (terminating). PA and all studied InsP(n) were separated within 25 min and detected by a conductivity detector. Simple sample preparation (acidic extraction), sufficient sensitivity, speed of analysis, and low running cost are important attributes of the electrophoretic methods. The method was used for the determination of PA and InsP(n) in barley varieties within an ongoing research project.
authors: Institute of Chemical Technology, Technická 5, Prague, Czech Republic. frantisek.kvasnicka@vscht.cz
authors: František
authors: F
authors: Kvasnička
authors: Jana
authors: J
authors: Copíková
authors: Rudolf
authors: R
authors: Sevčík
authors: Eliška
authors: E
authors: Václavíková
authors: Andriy
authors: A
authors: Synytsya
authors: Kateřina
authors: K
authors: Vaculová
authors: Michal
authors: M
authors: Voldřich
mesh_terms: D019075:Electrophoresis, Capillary; D001467:Hordeum / Q000737:chemistry*; D007295:Inositol Phosphates / Q000032:analysis*; D057625:Isotachophoresis; D010833:Phytic Acid / Q000032:analysis*; D012639:Seeds / Q000737:chemistry
chemical_list: D007295:Inositol Phosphates; D010833:Phytic Acid