title: 2D luminescence imaging of physiological wound oxygenation.
abstract: In cutaneous wound healing, the role of oxygen in vivo is poorly understood. We studied wound surface pO(2) during physiological wound healing in humans. Split-thickness skin graft donor sites (n=12) served as standardized wound models. Wound surface pO(2) was measured at 1, 6 and 14days after split-skin harvesting using two-dimensional luminescence lifetime imaging (2D-LLI) of palladium(II)-meso-tetraphenyl-tetrabenzoporphyrin (Pd-TPTBP) in polystyrene-co-acrylonitrile (PSAN) particles on transparent foils. In another experiment, we removed the stratum corneum (SC) on the volar forearm (n=10) by tape strippings to study the impact of the SC on the epidermal oxygen barrier. Split-skin donor site pO(2) significantly decreased during the time course of physiological healing. Regional differences in pO(2) within donor site wounds were visualized for the first time in literature. No difference was found in pO(2) before and after SC removal, showing that the SC is not a major constituent of the epidermal oxygen barrier.
authors: Department of Dermatology, University Hospital Regensburg, Regensburg, Germany. stephan.schreml@klinik.uni-regensburg.de
authors: Stephan
authors: S
authors: Schreml
authors: Robert J
authors: RJ
authors: Meier
authors: Otto S
authors: OS
authors: Wolfbeis
authors: Tim
authors: T
authors: Maisch
authors: Rolf-Markus
authors: RM
authors: Szeimies
authors: Michael
authors: M
authors: Landthaler
authors: Johannes
authors: J
authors: Regensburger
authors: Francesco
authors: F
authors: Santarelli
authors: Ingo
authors: I
authors: Klimant
authors: Philipp
authors: P
authors: Babilas
mesh_terms: D000181:Acrylonitrile / Q000737:chemistry; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004817:Epidermis / Q000378:metabolism; D005260:Female; D006801:Humans; D020100:Hydrogels / Q000737:chemistry; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects; D049408:Luminescent Agents / Q000737:chemistry / Q000378:metabolism; D008163:Luminescent Measurements / Q000379:methods*; D008297:Male; D008665:Metalloporphyrins / Q000737:chemistry / Q000378:metabolism; D008875:Middle Aged; D057054:Molecular Imaging / Q000379:methods*; D015335:Molecular Probes / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D010100:Oxygen / Q000378:metabolism*; D010313:Partial Pressure; D010316:Particle Size; D011137:Polystyrenes / Q000737:chemistry; D011140:Polyurethanes / Q000737:chemistry; D011145:Polyvinyls / Q000737:chemistry; D016038:Skin Transplantation; D014945:Wound Healing / Q000502:physiology*; D014947:Wounds and Injuries / Q000378:metabolism*; D055815:Young Adult
chemical_list: D020100:Hydrogels; D049408:Luminescent Agents; D008665:Metalloporphyrins; D015335:Molecular Probes; D011137:Polystyrenes; D011140:Polyurethanes; D011145:Polyvinyls; C015498:Saran; D000181:Acrylonitrile; D010100:Oxygen