title: Detection of hantaviruses in Brazilian rodents by SYBR-Green-based real-time RT-PCR.
abstract: Current knowledge of the pathogenic hantavirus indicates that wild rodents are its primary natural reservoir. Specific primers to detect the presence of viral genomes were developed using an SYBR-Green-based real-time RT-PCR protocol. One hundred sixty-four rodents native to the Atlantic Forest biome were captured in São Paulo State, Brazil, and their tissues were tested. The presence of hantavirus RNA was detected in sixteen rodents: three specimens of Akodon montensis, three of Akodon cursor, two of Necromys lasiurus, one of Juliomys sp., one of Thaptomys nigrita, five of Oligoryzomys nigripes, and one of Oryzomys sp. This SYBR Green real-time RT-PCR method for detection of hantavirus may be useful for surveying hantaviruses in Brazil.
authors: BSL 3 Laboratory of Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil. jansentequila@usp.br
authors: J
authors: J
authors: Araujo
authors: A
authors: A
authors: Pereira
authors: M S
authors: MS
authors: Nardi
authors: D A
authors: DA
authors: Henriques
authors: D A
authors: DA
authors: Lautenschalager
authors: L M
authors: LM
authors: Dutra
authors: T L
authors: TL
authors: Ometto
authors: R F
authors: RF
authors: Hurtado
authors: F
authors: F
authors: Maués
authors: A
authors: A
authors: Nava
authors: F A
authors: FA
authors: Morais
authors: C C
authors: CC
authors: Aires
authors: S
authors: S
authors: Favorito
authors: E L
authors: EL
authors: Durigon
mesh_terms: D000818:Animals; D001483:Base Sequence; D052160:Benzothiazoles; D001938:Brazil; D003959:Diamines; D004197:Disease Reservoirs / Q000821:virology*; D006476:Orthohantavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D009930:Organic Chemicals / Q000737:chemistry; D010802:Phylogeny; D011804:Quinolines; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods*; D012377:Rodentia / Q000821:virology*
chemical_list: D052160:Benzothiazoles; D003959:Diamines; D009930:Organic Chemicals; D011804:Quinolines; C098022:SYBR Green I