title: The transcription factor E4BP4 regulates the production of IL-10 and IL-13 in CD4+ T cells.
abstract: The immunoregulatory cytokine interleukin 10 (IL-10) is expressed mainly by T helper type 2 (T(H)2) cells but also by T(H)1 cells during chronic infection. Here we observed plasticity in the expression of IL-10 and IL-13 after chronic T(H)1 stimulation; furthermore, the expression of Il10 and Il13 was regulated by the transcription factor E4BP4. Chronically stimulated E4BP4-deficient (Nfil3(-/-); called 'E4bp4(-/-)' here) T(H)1 cells, regulatory T cells (T(reg) cells) and natural killer T cells (NKT cells) had attenuated expression of IL-10 and IL-13. Enforced expression of E4bp4 initiated the production of IL-10 and IL-13 by conventional T(H)1 cells. E4bp4(-/-) T(H)2 cells showed impairment of IL-10 production with no effect on IL-13. Our results indicate that E4BP4 has multiple functions in controlling the plasticity of IL-13 in T(H)1 cells and IL-10 in T(H)1 cells, T(H)2 cells, T(reg) cells and NKT cells.
authors: Laboratory for Signal Network, Research Center for Allergy and Immunology, RIKEN Yokohama Institute, Yokohama, Japan.
authors: Yasutaka
authors: Y
authors: Motomura
authors: Hiroshi
authors: H
authors: Kitamura
authors: Atsushi
authors: A
authors: Hijikata
authors: Yuko
authors: Y
authors: Matsunaga
authors: Koichiro
authors: K
authors: Matsumoto
authors: Hiromasa
authors: H
authors: Inoue
authors: Koji
authors: K
authors: Atarashi
authors: Shohei
authors: S
authors: Hori
authors: Hiroshi
authors: H
authors: Watarai
authors: Jinfang
authors: J
authors: Zhu
authors: Masaru
authors: M
authors: Taniguchi
authors: Masato
authors: M
authors: Kubo
mesh_terms: D000818:Animals; D001327:Autoimmune Diseases / Q000276:immunology*; D050976:Basic-Leucine Zipper Transcription Factors / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D024202:Electrophoretic Mobility Shift Assay; D005434:Flow Cytometry; D016753:Interleukin-10 / Q000276:immunology*; D018793:Interleukin-13 / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D011401:Promoter Regions, Genetic; D013047:Specific Pathogen-Free Organisms; D014158:Transcription, Genetic
chemical_list: D050976:Basic-Leucine Zipper Transcription Factors; D018793:Interleukin-13; C497441:Nfil3 protein, mouse; D016753:Interleukin-10