title: A comparison of triazole-forming bioconjugation techniques for constructing comb-shaped peptide-polymer bioconjugates.
abstract: The grafting-to of a peptide to a side chain functional polymer was investigated using "click" chemistry. Two click reactions were compared: the copper-free strain-promoted azide-alkyne 1,3-cycloaddition (SPAAC) and the traditional copper-catalyzed azide-alkyne 1,3-cycloaddition (CuAAC). For the resulting comb-shaped products, it was found that the steric bulk of the conjugation moiety used in SPAAC limits the degree of grafting for these highly dense systems, whereas CuAAC gives (near) quantitative functionalization.
authors: Institute for Molecules and Materials, Radboud University Nijmegen, Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands.
authors: Luiz A
authors: LA
authors: Canalle
authors: Matthijs
authors: M
authors: van der Knaap
authors: Mark
authors: M
authors: Overhand
authors: Jan C M
authors: JC
authors: van Hest
mesh_terms: D000480:Alkynes / Q000737:chemistry; D001386:Azides / Q000737:chemistry; D002384:Catalysis; D057930:Click Chemistry / Q000379:methods*; D003300:Copper / Q000737:chemistry; D061565:Cycloaddition Reaction; D006096:Gramicidin / Q000737:chemistry*; D008689:Methacrylates / Q000737:chemistry*; D015394:Molecular Structure; D011109:Polymethacrylic Acids / Q000737:chemistry*; D014230:Triazoles / Q000737:chemistry
chemical_list: D000480:Alkynes; D001386:Azides; D008689:Methacrylates; D011109:Polymethacrylic Acids; D014230:Triazoles; D006096:Gramicidin; D003300:Copper; C032802:hydroxypropyl methacrylate