title: Lipoic acid prevents steroid-induced osteonecrosis in rabbits.
abstract: The objective of this study was to investigate in vivo effects of lipoic acid (LA) in preventing steroid-induced osteonecrosis and the possible pathway in a rabbit model. Sixty rabbits were divided into 2 groups: rabbits were intraperitoneally injected with LA aqueous solution at 36 mg/kg of body weight per day for 4 weeks in Group A and rabbits were injected with physiologic saline (PS) as a control in Group B. At 2 weeks after starting treatment, they were intramuscularly injected once with 20 mg/kg of methylprednisolone acetate (MPSL). The femora were histopathologically examined for the presence of osteonecrosis. The plasma levels of total cholesterol (TC), low-density lipoprotein (LDL), high-density lipoprotein (HDL), glutathione (GSH), endothelin (ET) and malondialdehyde (MDA) were assayed at 2 weeks after the injection of MPSL. The incidence of osteonecrosis was significantly higher in Group B (73.1%) than in Group A (20.8%). The GSH level was higher in Group A than in Group B after the LA injection. The plasma MDA and ET levels were lower in Group A than in Group B at 2 weeks after the MPSL administration. Lipoic acid can prevent the development of steroid-induced osteonecrosis in rabbits. Inhibited oxidative stress and amendment of vascular endothelial dysfunction is a possible mechanism for this effect.
authors: Department of Orthopedics, Xiangya Hospital, Central-South University, Xiangya Road 87#, Kaifu, Changsha, Hunan, 410008, People's Republic of China.
authors: Bang-Bao
authors: BB
authors: Lu
authors: Kang-Hua
authors: KH
authors: Li
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D002784:Cholesterol / Q000097:blood; D019610:Cytoprotection; D004195:Disease Models, Animal; D016232:Endothelins / Q000097:blood; D005269:Femur / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D005978:Glutathione / Q000097:blood; D008075:Lipoproteins, HDL / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008775:Methylprednisolone / Q000031:analogs & derivatives*; D000077555:Methylprednisolone Acetate; D010020:Osteonecrosis / Q000097:blood / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D018384:Oxidative Stress / Q000187:drug effects; D020011:Protective Agents / Q000494:pharmacology*; D011817:Rabbits; D008063:Thioctic Acid / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D015415:Biomarkers; D016232:Endothelins; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D020011:Protective Agents; D000077555:Methylprednisolone Acetate; D008315:Malondialdehyde; D008063:Thioctic Acid; D002784:Cholesterol; D005978:Glutathione; D008775:Methylprednisolone