title: [Comparative proteomic characteristic of Mycoplasmas (Mollycutes)].
abstract: Saturating proteome identification and the study of post-translational protein modifications of Acholeplasma laidlawii using combination of single- and two-dimension gel electrophoresis followed by mass-spectrometry analysis have been carried out. Results were compared to the earlier identified proteome of Mycoplasma gallisepticum. It was found that M. gallisepticum and A. laidlawii express 61 and 58% of the annotated ORFs respectively. All subunits of DNA-polymerase III were identified during our study which indicates that our methods can detect single copies of a given protein per cell. Metabolic pathways of the respective mycoplasmas were compared further in this work.
authors: I A
authors: IA
authors: Demina
authors: M V
authors: MV
authors: Serebriakova
authors: V G
authors: VG
authors: Ladygina
authors: M A
authors: MA
authors: Galiamina
authors: N A
authors: NA
authors: Zhukova
authors: D G
authors: DG
authors: Alekseev
authors: G Iu
authors: GIu
authors: Fisunov
authors: V M
authors: VM
authors: Govorun
mesh_terms: D000128:Acholeplasma laidlawii / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D005798:Genes, Bacterial; D066298:In Vitro Techniques; D053858:Metabolic Networks and Pathways; D045686:Mycoplasma gallisepticum / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D020543:Proteome / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D020543:Proteome