title: A role for ZO-1 and PLEKHA7 in recruiting paracingulin to tight and adherens junctions of epithelial cells.
abstract: Paracingulin is a 160-kDa protein localized in the cytoplasmic region of epithelial tight and adherens junctions, where it regulates RhoA and Rac1 activities by interacting with guanine nucleotide exchange factors. Here, we investigate the molecular mechanisms that control the recruitment of paracingulin to cell-cell junctions. We show that paracingulin forms a complex with the tight junction protein ZO-1, and the globular head domain of paracingulin interacts directly with ZO-1 through an N-terminal region containing a conserved ZIM (ZO-1-Interaction-Motif) sequence. Recruitment of paracingulin to cadherin-based cell-cell junctions in Rat1 fibroblasts requires the ZIM-containing region, whereas in epithelial cells removal of this region decreases the junctional localization of paracingulin at tight junctions but not at adherens junctions. Depletion of ZO-1, but not ZO-2, reduces paracingulin accumulation at tight junctions. A yeast two-hybrid screen identifies both ZO-1 and the adherens junction protein PLEKHA7 as paracingulin-binding proteins. Paracingulin forms a complex with PLEKHA7 and its interacting partner p120ctn, and the globular head domain of paracingulin interacts directly with a central region of PLEKHA7. Depletion of PLEKHA7 from Madin-Darby canine kidney cells results in the loss of junctional localization of paracingulin and a decrease in its expression. In summary, we characterize ZO-1 and PLEKHA7 as paracingulin-interacting proteins that are involved in its recruitment to epithelial tight and adherens junctions, respectively.
authors: Department of Molecular Biology, University of Geneva, 4 Boulevard d'Yvoy, 1205 Geneva, Switzerland.
authors: Pamela
authors: P
authors: Pulimeno
authors: Serge
authors: S
authors: Paschoud
authors: Sandra
authors: S
authors: Citi
mesh_terms: D022005:Adherens Junctions / Q000378:metabolism*; D000818:Animals; D018938:Caco-2 Cells; D015820:Cadherins / Q000737:chemistry; D002352:Carrier Proteins / Q000378:metabolism*; D051177:Catenins / Q000737:chemistry; D003593:Cytoplasm / Q000378:metabolism; D003598:Cytoskeletal Proteins / Q000737:chemistry*; D003599:Cytoskeleton / Q000378:metabolism; D004285:Dogs; D004847:Epithelial Cells / Q000378:metabolism*; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D051381:Rats; D020798:Two-Hybrid System Techniques; D062826:Zonula Occludens-1 Protein; D000095002:Delta Catenin
chemical_list: C533277:CGNL1 protein, human; D015820:Cadherins; D002352:Carrier Proteins; D051177:Catenins; D003598:Cytoskeletal Proteins; D008565:Membrane Proteins; C533786:PLEKHA7 protein, human; D010750:Phosphoproteins; C569042:TJP1 protein, human; C569044:Tjp1 protein, rat; D062826:Zonula Occludens-1 Protein; D000095002:Delta Catenin