title: Expression and purification of bioactive high-purity recombinant mouse CXCL14 in Escherichia coli.
abstract: Mouse CXCL14/BRAK is a monocyte-selective chemokine which is expressed in almost all normal tissues. A flood of reports on its new functions of tumor suppression and fat metabolism modulation has left CXCL14 a potential therapeutic candidate for these diseases. Therefore, a simple accessible method is on demand for large-scale production of recombinant mouse CXCL14 protein for in vivo animal studies. Here, we introduce an efficient method for large-scale production of recombinant mouse CXCL14, by which an 18-mg protein is produced from 2-L Escherichia coli culture with good bioactivity and low level of endotoxin.
authors: Shanghai Municipality Key Laboratory of Veterinary Medicine and Biotechnology, School of Agriculture and Biology, Shanghai, People's Republic of China.
authors: Jingjing
authors: J
authors: Li
authors: Jin
authors: J
authors: Gao
authors: Sunita
authors: S
authors: Sah
authors: Uttam
authors: U
authors: Satyal
authors: Ruliang
authors: R
authors: Zhang
authors: Wei
authors: W
authors: Han
authors: Yan
authors: Y
authors: Yu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019743:Chemokines, CXC / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism; D002846:Chromatography, Affinity; D004731:Endotoxins / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
chemical_list: C517518:CXCL14 protein, mouse; D019743:Chemokines, CXC; D004731:Endotoxins; D011994:Recombinant Proteins