title: Dendrimer mediated transfer of engineered chromosomes.
abstract: Gene therapy encounters important problems such as insertional mutagenesis caused by the integration of viral vectors. These problems could be circumvented by the use of mammalian artificial chromosomes (MACs) that are unique and high capacity gene delivery tools. MACs were delivered into various target cell lines including stem cells by microcell-mediated chromosome transfer (MMCT), microinjection, and cationic lipid and dendrimer mediated transfers. MACs were also cleansed to more than 95% purity before transfer with an expensive technology. We present here a method by which MACs can be delivered into murine embryonic stem (ES) cells with a nonexpensive, less tedious, but still efficient way.
authors: Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary. katona@brc.hu
authors: Robert L
authors: RL
authors: Katona
mesh_terms: D000818:Animals; D016466:CHO Cells; D022221:Chromosomes, Artificial, Mammalian / Q000235:genetics* / Q000378:metabolism*; D002999:Clone Cells; D006224:Cricetinae; D003412:Cricetulus; D050091:Dendrimers / Q000378:metabolism*; D004351:Drug Resistance; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism; D018014:Gene Transfer Techniques; D005818:Genetic Engineering; D006801:Humans; D007621:Karyotyping; D008938:Mitosis
chemical_list: D050091:Dendrimers