title: First investigation of spider silk as a braided microsurgical suture.
abstract: Inhibition of axonal outgrowth accompanied by neuroma formation appears in microsurgical nerve repair as reaction to common microsuture materials like silk, nylon, or polyglycolic acid. In contrast, recent findings revealed advantages of spider silk fibers in guiding Schwann cells in nerve regeneration. Here, we asked if we could braid microsutures from native spider silk fibers. Microsutures braided of native spider dragline silk were manufactured, containing either 2 × 15 or 3 × 10 single fibres strands. Morphologic appearance was studied and tensile strength and stress-strain ratio (SSR) were calculated. The constructed spider silk sutures showed a median thickness of 25 μm, matching the USP definition of 10-0. Maximum load and tensile strength for both spider silk microsutures were significantly more than 2-fold higher than for nylon suture; SSR was 1.5-fold higher. All values except elasticity were higher in 3 × 10 strand sutures compared to 2 × 15 strand sutures, but not significantly. In this pilot study, we demonstrate the successful manufacture of microsutures from spider silk. With regards to the mechanical properties, these sutures were superior to nylon sutures. As spider silk displays high biocompatibility in nerve regeneration, its usage in microsurgical nerve repair should be considered.
authors: Department of Plastic, Hand, and Reconstructive Surgery, Medical School Hannover, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany. joernkuhbier@gmx.de
authors: Joern W
authors: JW
authors: Kuhbier
authors: Kerstin
authors: K
authors: Reimers
authors: Cornelia
authors: C
authors: Kasper
authors: Christina
authors: C
authors: Allmeling
authors: Anja
authors: A
authors: Hillmer
authors: Björn
authors: B
authors: Menger
authors: Peter M
authors: PM
authors: Vogt
authors: Christine
authors: C
authors: Radtke
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D004548:Elasticity; D005260:Female; D006801:Humans; D008422:Materials Testing; D008866:Microsurgery / Q000295:instrumentation*; D009416:Nerve Regeneration; D009493:Neurosurgery / Q000295:instrumentation*; D010865:Pilot Projects; D047011:Silk / Q000737:chemistry*; D013112:Spiders; D013537:Sutures; D013718:Tensile Strength
chemical_list: D001672:Biocompatible Materials; D047011:Silk