title: A PCR-based strategy for Dombrock screening in Brazilian blood donors reveals a novel allele: the DO* A-WL.
abstract: BACKGROUND
  Determination of the molecular basis underlying the antigens in the Dombrock blood group system has shown various rearrangements between the alleles associated with DO(*) A and DO(*) B. Based on this, we employed a PCR-based strategy to screen DO alleles (DO(*) A, DO(*) B, HY(*) 1, HY(*) 2 and JO) in Brazilians.
  
  
  METHODS
  We tested DNA of 278 Brazilian blood donors by PCR-RFLP on plates of 96 wells to determine the 793A/G (DO(*) A/DO(*) B), 323G/T (HY), 350C/T (JO) and 898C/G (HY(*) 1/HY(*) 2) single nucletide polymorphisms. In order to confirm the results sequence analysis was also performed.
  
  
  RESULTS
  When samples of these donors were analyzed, a novel allele combination, the DO(*) A allele (793A and 323G) associated with 898G was identified and designated as DO(*) A-WL allele. This new allele encoding 300Val is the same as HY(*) 1 at nucleotide 898 on the molecular background of DO(*) A. Among the 556 alleles analyzed by PCR-RFLP, 3 were DO(*) A-WL and 78 were DO(*) B-WL. This represents an overall frequency of 0.5% for DO(*) A-WL and 14% for DO(*) B-WL across the population studied.
  
  
  CONCLUSIONS
  Molecular screening of Brazilians revealed one novel allele, the DO(*) A-WL. Our data highlight the importance of testing a cohort of different populations to determine DO haplotypes and to establish reliable genotyping tests for predicting Do(a)/Do(b) status.
authors: Hemocentro, Faculdade de Medicina, Marília, Sao Paulo, Brazil. baleotti@famema.br
authors: Wilson
authors: W
authors: Baleotti
authors: Rodrigo Buzinaro
authors: RB
authors: Suzuki
authors: Milena
authors: M
authors: Polotto
authors: Marcelo Ortega
authors: MO
authors: Ruiz
authors: Antonio
authors: A
authors: Fabron
authors: Lilian
authors: L
authors: Castilho
mesh_terms: D036002:ADP Ribose Transferases / Q000235:genetics*; D000483:Alleles; D001782:Blood Donors / Q000145:classification*; D001789:Blood Group Antigens / Q000235:genetics*; D001938:Brazil; D005006:Ethnicity / Q000235:genetics*; D005820:Genetic Testing; D005838:Genotype; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide
chemical_list: D001789:Blood Group Antigens; D008565:Membrane Proteins; D036002:ADP Ribose Transferases; C105234:ART4 protein, human