title: Decellularized extracellular matrix derived from human adipose tissue as a potential scaffold for allograft tissue engineering.
abstract: Decellularized tissues composed of extracellular matrix (ECM) have been clinically used to support the regeneration of various human tissues and organs. Most decellularized tissues so far have been derived from animals or cadavers. Therefore, despite the many advantages of decellularized tissue, there are concerns about the potential for immunogenicity and the possible presence of infectious agents. Herein, we present a biomaterial composed of ECM derived from human adipose tissue, the most prevalent, expendable, and safely harvested tissue in the human body. The ECM was extracted by successive physical, chemical, and enzymatic treatments of human adipose tissue isolated by liposuction. Cellular components including nucleic acids were effectively removed without significant disruption of the morphology or structure of the ECM. Major ECM components were quantified, including acid/pepsin-soluble collagen, sulfated glycosaminoglycan (GAG), and soluble elastin. In an in vivo experiment using mice, the decellularized ECM graft exhibited good compatibility to surrounding tissues. Overall results suggest that the decellularized ECM containing biological and chemical cues of native human ECM could be an ideal scaffold material not only for autologous but also for allograft tissue engineering.
authors: Department of Chemical Engineering and Department of Bionanotechnology, Hanyang University, Ansan, Gyeonggi-do 426-791, Republic of Korea.
authors: Ji Suk
authors: JS
authors: Choi
authors: Beob Soo
authors: BS
authors: Kim
authors: Jun Young
authors: JY
authors: Kim
authors: Jae Dong
authors: JD
authors: Kim
authors: Young Chan
authors: YC
authors: Choi
authors: Hyun-Jin
authors: HJ
authors: Yang
authors: Kinam
authors: K
authors: Park
authors: Hee Young
authors: HY
authors: Lee
authors: Yong Woo
authors: YW
authors: Cho
mesh_terms: D000273:Adipose Tissue / Q000737:chemistry*; D000328:Adult; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D005109:Extracellular Matrix / Q000737:chemistry*; D005260:Female; D006801:Humans; D051379:Mice; D008813:Mice, Inbred ICR; D023822:Tissue Engineering / Q000379:methods*; D014184:Transplantation, Homologous; D055815:Young Adult
chemical_list: D001672:Biocompatible Materials