title: Simultaneous binding of anti-tuberculosis and anti-thrombosis drugs to a human transporter protein: a FRET study.
abstract: Although rifampicin (Rf) is one of the most effective antibiotics against infection caused by Mycobacterium tuberculosis, interaction of the drug with universal carrier protein in human blood plasma is not fully understood. Reduction of medicinal efficacy of other drugs, including anti-thrombosis drug warfarin (Wf), to the patients on Rf therapy also needs molecular understanding. In the present work we have studied interaction of Rf with one of the model carrier protein (human serum albumin). By using circular dichroism (CD) spectroscopy we have characterized the change in the secondary structure of the protein. The consequence of the simultaneous binding of the two drugs, Rf and Wf, on the structure of the protein has also been explored. Picosecond resolved Förster resonance energy transfer (FRET) from Wf to Rf explores possible binding sites of the anti-tuberculosis drug on the protein. In this report, we have discussed the potential problem of using the single tryptophan of the protein (Trp 214) as energy donor in FRET experiment for the characterization of the binding site of the drug Rf on the protein.
authors: Unit for Nano Science & Technology, S.N. Bose National Centre for Basic Sciences, Block JD, Sector III, Salt Lake, Kolkata 700 098, India.
authors: Priya
authors: P
authors: Rajdev
authors: Tanumoy
authors: T
authors: Mondol
authors: Abhinandan
authors: A
authors: Makhal
authors: Samir Kumar
authors: SK
authors: Pal
mesh_terms: D000995:Antitubercular Agents / Q000378:metabolism*; D002942:Circular Dichroism; D005343:Fibrinolytic Agents / Q000378:metabolism*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D006801:Humans; D012293:Rifampin / Q000378:metabolism*; D012709:Serum Albumin / Q000378:metabolism*; D014364:Tryptophan / Q000737:chemistry; D014859:Warfarin / Q000378:metabolism*
chemical_list: D000995:Antitubercular Agents; D005343:Fibrinolytic Agents; D012709:Serum Albumin; D014859:Warfarin; D014364:Tryptophan; D012293:Rifampin