title: Elevated serum levels of heat shock protein 70 can be detected after radiofrequency ablation.
abstract: Due to their adjuvant effect and their ability to chaperone tumor-associated peptides, heat shock proteins constitute a potent alarm signal for the immune system and can lead to activation of anti-tumor T-cell immunity. Radiofrequency ablation has been reported to induce heat shock protein expression especially that of heat shock protein 70 in sublethally damaged tumor cells. In this study, we evaluated the release of heat shock protein 70 into the serum of cancer-bearing patients directly after radiofrequency ablation. Sera of 22 patients undergoing radiofrequency ablation for the treatment of primary and secondary malignancies of the liver, kidney, and lung, as well as control sera of 20 patients undergoing diagnostic liver biopsy were analyzed using a manufactured heat shock protein 70 ELISA. A significant increase in serum levels of heat shock protein 70 was detectable in the patient cohort 1 day after radiofrequency ablation. More than a twofold increase was observed in nine out of 22 patients, which tended to correlate with favorable clinical outcome. No patient of the control group revealed a comparable increase. Radiofrequency ablation can lead to a release of heat shock protein 70 into the serum, which is transiently detectable 1 day after treatment. Elevated heat shock protein 70 serum levels may constitute a biomarker for favorable clinical outcome.
authors: Interfakultaeres Institut fuer Zellbiologie, Abteilung Immunologie, Auf der Morgenstelle 15, 72076, Tuebingen, Germany. sebastian.haen@med.uni-tuebingen.de
authors: Sebastian P
authors: SP
authors: Haen
authors: Cécile
authors: C
authors: Gouttefangeas
authors: Diethard
authors: D
authors: Schmidt
authors: Andreas
authors: A
authors: Boss
authors: Stephan
authors: S
authors: Clasen
authors: Alexandra
authors: A
authors: von Herbay
authors: Bora
authors: B
authors: Kosan
authors: Hermann
authors: H
authors: Aebert
authors: Philippe L
authors: PL
authors: Pereira
authors: Hans-Georg
authors: HG
authors: Rammensee
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000378:metabolism; D001706:Biopsy; D017115:Catheter Ablation; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000097:blood*; D006801:Humans; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000378:metabolism / Q000532:radiotherapy*; D016896:Treatment Outcome; D055815:Young Adult
chemical_list: D015415:Biomarkers; D018840:HSP70 Heat-Shock Proteins