title: Adeno-associated virus serotype 2 mediated transduction and coexpression of the human apoAI and SR-BI gene in HepG2 cells.
abstract: Cholesterol efflux is the first step in the reverse cholesterol transport (RCT) pathway, removing excess cholesterol from tissues, including the arterial wall, thus preventing the development of atherosclerosis. Adeno-associated virus (rAAV) has demonstrated significant promise as a DNA-delivery vector to treat serious human diseases. In this study, we constructed recombinant adeno-associated viruses coexpressing apoAI and SR-BI successfully, the double gene mRNA and protein were both strongly expressed in transduced HepG2 cells. A novel safe and efficient method of promoting the reverse cholesterol transport (RCT) may be established. These results may provide a new method for gene therapy of Arteriosclerosis.
authors: Experimental Hematology Laboratory, Department of Physiology, Xiang-Ya School of Medicine, Central South University, Changsha, 410078, China.
authors: Bingnan
authors: B
authors: Li
authors: Juan
authors: J
authors: Zhang
authors: Zhiyan
authors: Z
authors: Li
authors: Mengqun
authors: M
authors: Tan
mesh_terms: D000704:Analysis of Variance; D016632:Apolipoprotein A-I / Q000378:metabolism*; D050197:Atherosclerosis / Q000517:prevention & control*; D015153:Blotting, Western; D002784:Cholesterol / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D000229:Dependovirus; D015316:Genetic Therapy / Q000379:methods*; D056945:Hep G2 Cells; D006801:Humans; D015151:Immunoblotting; D010957:Plasmids / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051122:Scavenger Receptors, Class B / Q000378:metabolism*; D014161:Transduction, Genetic / Q000379:methods*
chemical_list: D016632:Apolipoprotein A-I; D017931:DNA Primers; C495124:SCARB1 protein, human; D051122:Scavenger Receptors, Class B; D002784:Cholesterol