title: Biocompatible carbohydrate-functionalized stainless steel surfaces: a new method for passivating biomedical implants.
abstract: A convenient method for passivating and functionalizing stainless steel is described. Several methods of coating stainless steel (SS) samples with silica were investigated and of these methods, a thin (less than 15 nm thick) layer of silica created by atomic layer deposition (ALD) was found to give superior performance in electrochemical testing. These interfaces were then used as a platform for further functionalization with molecules of biological interest. Specifically, the SS samples were functionalized with biologically significant carbohydrates [N-acetyl-D-glucosamine (GlcNAc) and D-galactose (Gal)] that contain trialkoxysilane derivatives as chemical handles for linking to the surface. The presence and biological availability of these moieties on the silica coated SS were confirmed by XPS analysis and an enzyme-linked lectin assay (ELLA) using complementary lectins that specifically recognize the surface-bound carbohydrate. This method has the potential of being adapted to the functionalization of stainless steel biomedical implants with other biologically relevant carbohydrates.
authors: NRC National Institute for Nanotechnology, University of Alberta, Edmonton, Alberta T6G2G2, Canada.
authors: Anne M
authors: AM
authors: Slaney
authors: Vincent A
authors: VA
authors: Wright
authors: Peter J
authors: PJ
authors: Meloncelli
authors: Kenneth D
authors: KD
authors: Harris
authors: Lori J
authors: LJ
authors: West
authors: Todd L
authors: TL
authors: Lowary
authors: Jillian M
authors: JM
authors: Buriak
mesh_terms: D002241:Carbohydrates / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D019736:Prostheses and Implants; D012821:Silanes / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D013193:Stainless Steel / Q000737:chemistry*
chemical_list: D002241:Carbohydrates; D020099:Coated Materials, Biocompatible; D012821:Silanes; D013193:Stainless Steel; D011092:Polyethylene Glycols; D012822:Silicon Dioxide