title: [Genetically encoded photoimmunosensitizer].
abstract: Photosensitizer-antibody conjugates are successfully used for targeted elimination of cancer cells bearing specific membrane markers. This method is known as photoimmunotherapy. However, chemical conjugation of photosensitizer and antibody poses a number of complications such as low reproducibility, aggregation and unconjugated photosensitizer impurities. Here we report a fully genetically encoded photoimmunosensitizer, consisting of an anti-HER2/neu miniantibody 4D5scFv and a phototoxic fluorescent protein KillerRed. Both domains in this photoimmunosensitizer retained their functional qualities - high affinity for HER2/neu antigen and phototoxicity respectively. 4D5scFv-KillerRed fusion protein showed high specificity for HER2/neu-over-expressing cells and effectively lowered their viability upon illumination.
authors: E O
authors: EO
authors: Serebrovskaia
authors: O A
authors: OA
authors: Stremovskiĭ
authors: D M
authors: DM
authors: Chudakov
authors: K A
authors: KA
authors: Luk'ianov
authors: S M
authors: SM
authors: Deev
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D049452:Green Fluorescent Proteins / Q000235:genetics*; D006801:Humans; D000922:Immunotoxins / Q000235:genetics* / Q000302:isolation & purification / Q000494:pharmacology; D008027:Light; D017319:Photosensitizing Agents / Q000302:isolation & purification / Q000494:pharmacology; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000494:pharmacology; D057127:Single-Chain Antibodies / Q000235:genetics*
chemical_list: D049452:Green Fluorescent Proteins; D000922:Immunotoxins; D017319:Photosensitizing Agents; D018719:Erb-b2 Receptor Tyrosine Kinases; D011994:Recombinant Proteins; D057127:Single-Chain Antibodies; C508771:killer red protein, Anthomedusae; C508053:ERBB2 protein, human