title: Matrilin-1 expression is increased in the vertebral column of Atlantic salmon (Salmo salar L.) individuals displaying spinal fusions.
abstract: We have previously characterized the development of vertebral fusions induced by elevated water temperature in Atlantic salmon. Molecular markers of bone and cartilage development together with histology were used to understand the complex pathology and mechanism in the development of this spinal malformation. In this study, we wanted to use proteomics, a non-hypothetical approach to screen for possible new markers involved in the fusion process. Proteins extracted from non-deformed and fused vertebrae of Atlantic salmon were therefore compared by two-dimensional electrophoresis (2DE) and MALDI-TOF analysis. Data analysis of protein spots in the 2DE gels demonstrated matrilin-1, also named cartilage matrix protein, to be the most highly up-regulated protein in fused compared with non-deformed vertebrae. Furthermore, real-time PCR analysis showed strong up-regulation of matrilin-1 mRNA in fused vertebrae. Immunohistochemistry demonstrated induced matrilin-1 expression in trans-differentiating cells undergoing a metaplastic shift toward chondrocytes in fusing vertebrae, whereas abundant expression was demonstrated in cartilaginous tissue and chordocytes of both non-deformed and fused vertebrae. These results identifies matrilin-1 as a new interesting candidate in the fusion process, and ratify the use of proteomic as a valuable technique to screen for markers involved in vertebral pathogenesis.
authors: Nofima Mat AS, Ås, Norway. mona.pedersen@nofima.no
authors: Mona E
authors: ME
authors: Pedersen
authors: Harald
authors: H
authors: Takle
authors: Elisabeth
authors: E
authors: Ytteborg
authors: Eva
authors: E
authors: Veiseth-Kent
authors: Grethe
authors: G
authors: Enersen
authors: Ellen
authors: E
authors: Færgestad
authors: Grete
authors: G
authors: Baeverfjord
authors: Kirsten O
authors: KO
authors: Hannesson
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D054338:Cell Transdifferentiation; D015180:Electrophoresis, Gel, Two-Dimensional; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D029941:Fish Proteins / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism*; D016916:Joint Deformities, Acquired / Q000378:metabolism*; D064235:Matrilin Proteins; D020317:Salmo salar / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013131:Spine / Q000378:metabolism* / Q000473:pathology
chemical_list: D015415:Biomarkers; D016326:Extracellular Matrix Proteins; D029941:Fish Proteins; D006023:Glycoproteins; D064235:Matrilin Proteins