title: Multi-drug resistance and characteristic of integrons in Shigella spp. isolated from China.
abstract: OBJECTIVE
  To investigate the characteristic of integrons and the relationship between integrons and antimicrobial resistance in Shigella spp.
  
  
  METHODS
  Ninety Shigella strains (83 S. flexneri and 7 S. sonnei) were isolated from the stools of patients in China. Susceptibility to 8 antimicrobials was tested for all isolated strains. PCR, RFLP and sequencing analysis of integrons were applied to all of them.
  
  
  RESULTS
  High prevalence of multi-drug resistance (95.6%) was identified. Of the isolates 79 (87.8%) carried integrase genes of class 1 integron (3.3%), class 2 integron (10.0%) or both (74.4%). No intI3 was detected in the tested isolates. The prevalence of intI2 was significantly higher in isolates with multi-drug resistance to at least 3 antibiotics than that in isolates with resistance to 2 and less antibiotics (P<0.05). Gene cassettes dfrA17-aadA5, dfrA12-orfF-aadA2 of class 1 integron and dfrA1-sat1-aadA1 of class 2 integron were identified.
  
  
  CONCLUSIONS
  The class 2 integron may play a role in the emergence of multi-drug resistance in Shigella spp.
authors: Department of Environmental Health, School of Public Health, Zhengzhou University, Zhengzhou 450001, Henan China.
authors: Jingyuan
authors: J
authors: Zhu
authors: Guangcai
authors: G
authors: Duan
authors: Haiyan
authors: H
authors: Yang
authors: Qingtang
authors: Q
authors: Fan
authors: Yuanlin
authors: Y
authors: Xi
mesh_terms: D001483:Base Sequence; D002681:China; D017931:DNA Primers; D004352:Drug Resistance, Microbial; D018432:Drug Resistance, Multiple; D032023:Integrons; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012760:Shigella / Q000187:drug effects* / Q000235:genetics
chemical_list: D017931:DNA Primers