title: Effect of low level laser on sutured wound healing in rats.
abstract: OBJECTIVE
  To evaluate the effect of low-level laser therapy (LLLT) ë904 nm on healing of surgical wounds in rats.
  
  
  METHODS
  Forty male Wistar rats were used, divided into four groups, underwent incision along the lines Alba covering skin, subcutaneous and muscle abdominal, sutured continuously for nylon 5-0. Eight and fifteen days after the surgery process, the repairing area was removed and histological sections were stained with hematoxylin-eosin to assess cellularity inflammatory, Masson's Trichrome and Picrossirus to quantify the collagen fibers and immunohistochemical technique for counting newly formed vessels. The data were compared statistically using analysis of variance ANOVA, with a "post-hoc Tukey test, p <0.05.
  
  
  RESULTS
  Low-level laser therapy reduced the intensity of the inflammatory reaction and influenced the dynamic of the immunoinflammatory response by inducing switching of the leukocyte infiltration pattern (neutrophilic to lymphoplasmacytic infiltration). Also stimulate the deposition and enhance the organization of collagen fibers, featuring a delicate collagen type III. Furthermore, it appeared to a significant increase in the average number of newly formed vessels (p = 0.00 and p = 0.02, respectively).
  
  
  CONCLUSIONS
  Low-level laser therapy resulted in modulate of the inflammatory response, enhanced deposition of collagen fibers and increase in the average number of newly formed vessels.
authors: Department of Experimental Surgical, Federal University of Sergipe, Aracaju, SE, Brazil. vamelo@infonet.com.br
authors: Valdinaldo Aragão de
authors: VA
authors: Melo
authors: Denise Cristina Santos dos
authors: DC
authors: Anjos
authors: Ricardo
authors: R
authors: Albuquerque Júnior
authors: Daniel Barreto
authors: DB
authors: Melo
authors: Fábio Ursulino Reis
authors: FU
authors: Carvalho
mesh_terms: D000818:Animals; D003094:Collagen / Q000528:radiation effects; D028022:Low-Level Light Therapy / Q000379:methods*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013536:Suture Techniques; D014945:Wound Healing / Q000528:radiation effects*
chemical_list: D003094:Collagen