title: Screening target specificity of siRNAs by rapid amplification of cDNA ends (RACE) for non-sequenced species.
abstract: RNA interference (RNAi) is the process of sequence-specific posttranslational gene silencing triggered by double-stranded RNAs (dsRNAs). RNAi is a widely used approach for studying gene function. However, studies have shown that using siRNA can lead to off-target effects when the siRNA contains sufficient sequence identity to non-target mRNA sequences. One of the important steps in designing dsRNA is verification that it has sequence identity to only the target mRNA. In this report, we propose an approach for primary screening dsRNAs for potential off-target effects by using rapid amplification of cDNA ends. This method can be especially useful for model systems using species that have limited availability of sequence data.
authors: Neuroscience Group, Division of Basic Biomedical Sciences, University of South Dakota School of Medicine, 414 E. Clark St., Vermillion, SD 57069, USA. boris.sabirzhanov@gmail.com
authors: Boris
authors: B
authors: Sabirzhanov
authors: Inna B
authors: IB
authors: Sabirzhanova
authors: Joyce
authors: J
authors: Keifer
mesh_terms: D000818:Animals; D018076:DNA, Complementary / Q000235:genetics; D021141:Nucleic Acid Amplification Techniques; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics*; D014426:Turtles
chemical_list: D018076:DNA, Complementary; D012330:RNA, Double-Stranded; D034741:RNA, Small Interfering