title: Diminished AMPK signaling response to fasting in thioredoxin-interacting protein knockout mice.
abstract: Thioredoxin-interacting protein (Txnip) knockout (TKO) mice exhibit impaired response to fasting. Herein, we showed that activation of adenine monophosphate-activated protein kinase and cellular AMP levels were diminished in the heart and soleus muscle but not in gastrocnemius muscle of fasting TKO mice. Similarly, glycogen content in fasted TKO mice was increased in oxidative muscles but was not different in glycolytic muscles. These data suggest Txnip deficiency has a higher impact on oxidative muscle than glycolytic muscles and provide new insights into the metabolic role of Txnip.
authors: Department of Biology, BioScience Center, San Diego State University, San Diego, CA 92182, USA.
authors: Allen M
authors: AM
authors: Andres
authors: Eric P
authors: EP
authors: Ratliff
authors: Sowbarnika
authors: S
authors: Sachithanantham
authors: Simon T
authors: ST
authors: Hui
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism*; D000249:Adenosine Monophosphate / Q000378:metabolism; D000620:Aminoimidazole Carboxamide / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D015153:Blotting, Western; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D004789:Enzyme Activation; D005215:Fasting / Q000097:blood / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D051272:Glucose Transporter Type 1 / Q000235:genetics / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism; D007004:Hypoglycemic Agents / Q000494:pharmacology; D007328:Insulin / Q000097:blood; D051379:Mice; D018345:Mice, Knockout; D018482:Muscle, Skeletal / Q000187:drug effects / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D010766:Phosphorylation; D011768:Pyruvate Dehydrogenase Complex / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012265:Ribonucleotides / Q000494:pharmacology; D012694:Serine / Q000378:metabolism; D015398:Signal Transduction; D013879:Thioredoxins / Q000235:genetics / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D051272:Glucose Transporter Type 1; D007004:Hypoglycemic Agents; D007328:Insulin; D011768:Pyruvate Dehydrogenase Complex; D012265:Ribonucleotides; C495678:Slc2a1 protein, mouse; C445672:Txnip protein, mouse; D000620:Aminoimidazole Carboxamide; D000249:Adenosine Monophosphate; D012694:Serine; D013879:Thioredoxins; D006003:Glycogen; D055372:AMP-Activated Protein Kinases; C031143:AICA ribonucleotide; D005947:Glucose