title: Soluble chemokine receptor CXCR4 is present in human sera.
abstract: A soluble form of the chemokine receptor CXCR4 was detected in human sera by isoelectric focusing and Western blotting. Sera of patients and normal subjects were analyzed using a panel of specific antibodies. Compared with controls, high levels of serum CXCR4 were found in patients with inflammatory bowel diseases. Serum CXCR4 levels in the majority of HIV patients were similar to those in healthy controls. A sensitive polyclonal antibody was developed in rabbit immunized with a maltose binding protein (MBP) construct expressing the full-length CXCR4. Using anti-MBPCXCR4 antibody, the level of CXCR4 in sera of a majority of patients with fibrosis was very low. The potential of serum CXCR4 as a new diagnostic biomarker warrants further investigation.
authors: Fédération de Biochimie, Hôpital Edouard Herriot, CHU-Lyon, France. etienne.malvoisin@chu-lyon.fr
authors: Etienne
authors: E
authors: Malvoisin
authors: Jean-Michel
authors: JM
authors: Livrozet
authors: Djamila
authors: D
authors: Makloufi
authors: Nadine
authors: N
authors: Vincent
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D015415:Biomarkers / Q000097:blood; D015153:Blotting, Western; D015658:HIV Infections / Q000378:metabolism; D006801:Humans; D007525:Isoelectric Focusing; D058619:Maltose-Binding Proteins / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D019718:Receptors, CXCR4 / Q000097:blood* / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism
chemical_list: D000906:Antibodies; D015415:Biomarkers; D058619:Maltose-Binding Proteins; D019718:Receptors, CXCR4; D011993:Recombinant Fusion Proteins