title: Targeting Glut1-overexpressing MDA-MB-231 cells with 2-deoxy-D-g1ucose modified SPIOs.
abstract: Glucose transporter (Glut), a cellular transmembrane receptor, plays a key role in cell glucose metabolism and is linked to a poor prognosis in various human cancers. In this study, we prepared γ-Fe(2)O(3) NPs coated with DMSA, in which modified with 2-DG, then γ-Fe(2)O(3)@DMSA-DG NPs was constructed. The specific interactions between Glut1-overexpressing tumor cells (MDA-MB-231) and γ-Fe(2)O(3)@DMSA-DG NPs were observed using Prussian blue staining and transmission electron microscope (TEM), and found that γ-Fe(2)O(3)@DMSA-DG NPs were absorbed targetedly by the cells. Furthermore, the capacity of transporting SPIOs into tumor cells using these γ-Fe(2)O(3)@DMSA-DG NPs was evaluated with a 1.5 T clinical magnetic resonance imaging (MRI) scanner. It was found that the acquired MRI T2 signal intensity of MDA-MB-231 cells that were treated with the γ-Fe(2)O(3)@DMSA-DG NPs decreased significantly, and it was inhibited by competition with antibody of Glut1. Our results suggest that γ-Fe(2)O(3)@DMSA-DG NPs are a useful targeting to Glut1-overexpressing tumor cells in vitro and that γ-Fe(2)O(3)@DMSA-DG NPs may serve as a MRI-targeted tumor agent for better tumor imaging.
authors: Department of Radiology, The Affiliated Renmin Hospital, Jiangsu University, Zhenjiang, Jiangsu 212002, China. xhongshan@hotmail.com
authors: Xiu Hong
authors: XH
authors: Shan
authors: Hui
authors: H
authors: Hu
authors: Fei
authors: F
authors: Xiong
authors: Ning
authors: N
authors: Gu
authors: Xin Dong
authors: XD
authors: Geng
authors: Wei
authors: W
authors: Zhu
authors: Jiang
authors: J
authors: Lin
authors: Ya Fei
authors: YF
authors: Wang
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology*; D045744:Cell Line, Tumor; D003287:Contrast Media / Q000493:pharmacokinetics; D003847:Deoxyglucose / Q000493:pharmacokinetics*; D003911:Dextrans / Q000493:pharmacokinetics*; D051272:Glucose Transporter Type 1 / Q000378:metabolism*; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*; D058185:Magnetite Nanoparticles; D015854:Up-Regulation
chemical_list: D003287:Contrast Media; D003911:Dextrans; D051272:Glucose Transporter Type 1; D058185:Magnetite Nanoparticles; C495677:SLC2A1 protein, human; C097921:ferumoxtran-10; D003847:Deoxyglucose