title: [Establishment of a correlated cell model of oral lichen planus in vitro].
abstract: OBJECTIVE
  To establish a correlated cocultured model of keratinocytes and T lymphocytes in vitro to simulate the immune response circumstance of oral lichen planus(OLP) lesions.
  
  
  METHODS
  Keratinocytes prepared with enzyme digestion from normal oral mucosa and T lymphocytes prepared with negative isolation procedure by magnetic beads were co-cultured. Proliferation of T lymphocytes in cocultured cells was detected using MTT assay and the expression of IFN-γ was detected by ELISA. All the results were analyzed with independent samples t test using SAS6.12 software package.
  
  
  RESULTS
  The proliferation level of T lymphocytes in the cocultured group of T lymphocytes and keratinocytes which expressed PD-L1 and PD-L2 was higher than that in the group of T lymphocytes (P < 0.05). The expression of IFN-γ in the cocultured group was significantly higher than that in the group of T lymphocytes (P < 0.01).
  
  
  CONCLUSIONS
  The cocultured model of T lymphocytes and keratinocytes which express PD-L1 and PD-L2 can simulate the immune response circumstance of OLP lesions to a certain extent. Supported by National Natural Science Foundation of China (30872888),Research Fund of Science and Technology Commission of Shanghai Municipality(08DZ2271100) and Shanghai Leading Academic Discipline Project (S30206).
authors: Department of Oral Medicine, Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratary of Stomatology, Shanghai 200011, China. lq.11@163.com
authors: Qin
authors: Q
authors: Li
authors: Guan-huan
authors: GH
authors: Du
authors: Guo-yao
authors: GY
authors: Tang
mesh_terms: D002478:Cells, Cultured; D006801:Humans; D066298:In Vitro Techniques; D015603:Keratinocytes; D017676:Lichen Planus, Oral; D009061:Mouth Mucosa; D013601:T-Lymphocytes