title: Results of molecular diagnostic assays targeting feline herpesvirus-1 and feline calicivirus in adult cats administered modified live vaccines.
abstract: In this pilot study, 12 adult, gang-housed cats that were known to be previously exposed (n=12) to feline herpesvirus-1 (FHV-1) and/or vaccinated against (n=2) feline calicivirus (FCV) and FHV-1 were randomly assigned to one of two groups of six cats each. Nasal and pharyngeal samples were collected from each cat on days -7, -3, and 0 prior to vaccination and on days 3, 7, 10, 14, 17, 21, and 28 after vaccination with an FHV-1, FCV, and panleukopenia (FVRCP) vaccine developed for intranasal (six cats) or parenteral (six cats) use. FHV-1 DNA was amplified from 1/12 cats (1/69 samples; 1.4%) prior to vaccination and 2/12 cats after vaccination (2/154 samples; 1.3%). FCV RNA was amplified from 2/12 cats (2/69 samples; 2.9%) prior to vaccination and 7/12 cats (12/154 samples; 7.8%) after vaccination. Positive molecular diagnostic assay results for FHV-1 and FCV were uncommon prior to or after vaccination in these cats.
authors: Department of Clinical Sciences, Colorado State University, Fort Collins, CO 80523, USA. rgallie@colostate.edu
authors: Rebecca A
authors: RA
authors: Ruch-Gallie
authors: Julia K
authors: JK
authors: Veir
authors: Jennifer R
authors: JR
authors: Hawley
authors: Michael R
authors: MR
authors: Lappin
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D017250:Caliciviridae Infections / Q000175:diagnosis / Q000662:veterinary / Q000821:virology; D017927:Calicivirus, Feline / Q000276:immunology; D002371:Cat Diseases / Q000175:diagnosis* / Q000517:prevention & control / Q000821:virology*; D002415:Cats; D005254:Feline Panleukopenia / Q000175:diagnosis / Q000517:prevention & control / Q000821:virology; D028301:Feline Panleukopenia Virus / Q000276:immunology; D005260:Female; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000662:veterinary / Q000821:virology; D008297:Male; D009297:Nasal Mucosa / Q000821:virology; D010614:Pharynx / Q000821:virology; D010865:Pilot Projects; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012367:RNA, Viral / Q000032:analysis*; D011897:Random Allocation; D012141:Respiratory Tract Infections / Q000175:diagnosis / Q000517:prevention & control / Q000662:veterinary* / Q000821:virology; D018140:Varicellovirus / Q000276:immunology; D014765:Viral Vaccines
chemical_list: D000914:Antibodies, Viral; D012367:RNA, Viral; D014765:Viral Vaccines