title: [Determination of two bio-active compounds in Campylotropis hirtella (Franch.) Schindl. using reversed-phase high performance liquid chromatography].
abstract: A method of reversed-phase high performance liquid chromatography (RP-HPLC) using diode array detection (DAD) was developed for the quantitative determination of 3'-geranyl-5,7,4'-trihydroxyisoflavone (compound 1) and 8,9-dihydroxy-1-methoxy-[6',6'-dimethylpyrano(2',3': 2,3)]pterocarpene (compound 2) in Campylotropis hirtella. The separation and quantification were achieved using an Agilent Zorbax SB-C18 column (250 mm x 4.6 mm, 5 microm), and mobile phases of acetonitrile and 0. 1% formic acid with gradient elution at a flow rate of 1.0 mL/min and 30 degrees C. The calibration curves for compounds 1 and 2 were linear in the ranges of 4.4-13.2 microg and 0.428-1.284 microg, respectively. The recoveries were 99.65% and 99.11% with the relative standard deviations of 1.83% and 2.59% (n=5), respectively. This RP-HPLC-DAD method is rather simple, accurate and convenient. It can be used for the quantitative determination of the active flavonoids in Campylotropis hirtella (Franch.) Schindl.
authors: School of Chinese Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors: Qing
authors: Q
authors: Tan
authors: Qingyao
authors: Q
authors: Shou
authors: Sheng
authors: S
authors: Zhang
authors: Zhengwu
authors: Z
authors: Shen
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007887:Fabaceae / Q000737:chemistry*; D007529:Isoflavones / Q000032:analysis* / Q000302:isolation & purification; D015394:Molecular Structure; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*
chemical_list: D007529:Isoflavones