title: The two component adjuvant IC31® potentiates the protective immunity induced by a dengue 2 recombinant fusion protein in mice.
abstract: Here we evaluated the suitability of the synthetic adjuvant IC31® to potentiate the protective capacity of PD5 protein (domain III of the envelope protein of dengue 2 virus fused to the carrier protein P64k). Unlike Alum, PD5 mixed with IC31® induced complete protection against virus challenge in mice and increased IFN-γ secretion after in vitro re-stimulation. The induced antibody response was highly specific to the homologous serotype and showed both IgG1 and IgG2a subtypes. IC31® is a promising adjuvant for PD5 recombinant protein based vaccination against dengue. Future work should address the suitability of PD5/IC31® formulations in non-human primate models.
authors: Department of Virology, PAHO/WHO Collaborating Center for the Study of Dengue and its Vector, Pedro Kourí Tropical Medicine Institute, Autopista Novia del Mediodía, km 6 ½ P.O. Box 601 Marianao 13, Havana, Cuba.
authors: Lidice
authors: L
authors: Bernardo
authors: Alequis
authors: A
authors: Pavón
authors: Lisset
authors: L
authors: Hermida
authors: Lázaro
authors: L
authors: Gil
authors: Iris
authors: I
authors: Valdés
authors: Sheila
authors: S
authors: Cabezas
authors: Ramón
authors: R
authors: Linares
authors: Mayling
authors: M
authors: Alvarez
authors: Ricardo
authors: R
authors: Silva
authors: Gerardo
authors: G
authors: Guillén
authors: Eszter
authors: E
authors: Nagy
authors: Petra
authors: P
authors: Schlick
authors: María G
authors: MG
authors: Guzmán
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000276:immunology*; D003715:Dengue / Q000517:prevention & control*; D053059:Dengue Vaccines / Q000008:administration & dosage / Q000276:immunology*; D003716:Dengue Virus / Q000276:immunology*; D004338:Drug Combinations; D005260:Female; D007074:Immunoglobulin G / Q000097:blood; D007371:Interferon-gamma / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009838:Oligodeoxyribonucleotides / Q000008:administration & dosage*; D009842:Oligopeptides / Q000008:administration & dosage*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D000956:Antigens, Viral; D053059:Dengue Vaccines; D004338:Drug Combinations; C544002:IC31 adjuvant; D007074:Immunoglobulin G; D009838:Oligodeoxyribonucleotides; D009842:Oligopeptides; D011993:Recombinant Fusion Proteins; D014759:Viral Envelope Proteins; D007371:Interferon-gamma