title: Preliminary results of combining low frequency low intensity ultrasound and liposomal drug delivery to treat tumors in rats.
abstract: Ultrasound is a convenient trigger for site-specific drug delivery in cancer therapy. Nano-sized liposomes formulated from soy phosphatidyl choline, cholesterol, 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[carboxy(polyethylene glycol)-2000] and alpha-tocopherol were loaded with Doxorubicin (Dox) using a pH gradient. The liposomal suspension was infused through the tail vein of BDIX rats possessing bilateral intradermal DHD/K12 tumors on their hind legs. Then 20-kHz ultrasound was applied to only one of the tumors for 15 minutes. This therapy was repeated weekly for 4 weeks. The results showed that in five of six rats, the tumors regressed to non-measurable size within 4 weeks. A paired comparison of the normalized size of the insonated and non-insonated tumors in the same rat indicated that the insonated tumors were smaller (p < 0.0001, n = 6 rats, 21 pairs). This observation has significant potential for non-invasive site-specific therapy of solid tumors.
authors: Chemical Engineering Department, Brigham Young University, Provo, Utah 84602, USA.
authors: William G
authors: WG
authors: Pitt
authors: Ghaleb A
authors: GA
authors: Husseini
authors: Beverly L
authors: BL
authors: Roeder
authors: David J
authors: DJ
authors: Dickinson
authors: David R
authors: DR
authors: Warden
authors: Jonathan M
authors: JM
authors: Hartley
authors: Peter W
authors: PW
authors: Jones
mesh_terms: D000818:Animals; D004058:Diffusion; D004317:Doxorubicin / Q000008:administration & dosage* / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry* / Q000528:radiation effects*; D009374:Neoplasms, Experimental / Q000188:drug therapy* / Q000473:pathology*; D010865:Pilot Projects; D051381:Rats; D013010:Sonication; D016896:Treatment Outcome
chemical_list: D008081:Liposomes; D004317:Doxorubicin