title: Simultaneous quantification of triazolam and its metabolites in human urine by liquid chromatography-tandem mass spectrometry.
abstract: A simple, simultaneous, sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS-MS) method for the determination of triazolam and its metabolites, α-hydroxytriazolam (α-OHTRZ) and 4-hydroxytriazolam (4-OHTRZ), in human urine was developed and validated. Triazolam-d4 was used as the internal standard (IS). This analysis was carried out on a Thermo(®) C(18) column, and the mobile phase was composed of acetonitrile/H(2)O/formic acid (35:65:0.2, v/v/v). Detection was performed on a triple-quadrupole tandem MS using positive ion mode electrospray ionization, and quantification was performed by multiple reaction monitoring mode. The MS-MS ion transitions monitored were m/z 343.1 → 308.3, 359.0 → 331.0, 359.0 → 111.2, and 347.0 → 312.0 for triazolam, α-OHTRZ, 4-OHTRZ, and triazolam-d(4), respectively. The lower limits of quantification of the analytical method were 0.5 ng/mL for triazolam, 5 ng/mL for α-OHTRZ, and 0.5 ng/mL for 4-OHTRZ. The within- and between-run precisions were less than 15%, and accuracy was -12.33% to 9.76%. The method was proved to be accurate and specific, and it was applied to a urinary excretion study of triazolam in healthy Chinese volunteers.
authors: Department of Disaster Management, Taiwan Police College, 153 Shing-Lung Rd, Section 3, Taipei, Taiwan.
authors: Ryh-Nan
authors: RN
authors: Pan
authors: Cheng-Huei
authors: CH
authors: Hsiong
authors: Pei-Wei
authors: PW
authors: Huang
authors: Wei-Shiuan
authors: WS
authors: Tsai
authors: Wen-Yi
authors: WY
authors: Li
authors: Li-Heng
authors: LH
authors: Pao
mesh_terms: D000759:Adjuvants, Anesthesia / Q000737:chemistry / Q000652:urine*; D000328:Adult; D002853:Chromatography, Liquid; D006801:Humans; D008297:Male; D015813:Substance Abuse Detection / Q000379:methods*; D053719:Tandem Mass Spectrometry; D014229:Triazolam / Q000031:analogs & derivatives* / Q000737:chemistry / Q000652:urine*
chemical_list: D000759:Adjuvants, Anesthesia; D014229:Triazolam; C028638:alpha-hydroxytriazolam; C028637:4-hydroxytriazolam