title: Metastasis-associated protein 1 induces VEGF-C and facilitates lymphangiogenesis in colorectal cancer.
abstract: OBJECTIVE
  To study the correlation between high metastasis-associated protein 1 (MTA1) expression and lymphangiogenesis in colorectal cancer (CRC) and its role in production of vascular endothelial growth factor-C(VEGF-C).
  
  
  METHODS
  Impact of high MTA1 and VEGF-C expression levels on disease progression and lymphovascular density (LVD, D2-40-immunolabeled) in 81 cases of human CRC was evaluated by immunohistochemistry. VEGF-C mRNA and protein expressions in human LoVo and HCT116 cell lines were detected by real-time polymerase chain reaction and Western blotting, respectively, with a stable expression vector or siRNA.
  
  
  RESULTS
  The elevated MTA1 and VEGF-C expression levels were correlated with lymph node metastasis and Dukes stages (P < 0.05). Additionally, high MTA1 expression level was correlated with a large tumor size (P < 0.05). A significant correlation was found between MTA1 and VEGF-C protein expressions in tumor cells (r = 0.371, P < 0.05). Similar to the VEGF-C expression level, high MTA1 expression level was correlated with high LVD in CRC (P < 0.05). Furthermore, over-expression of MTA1 significantly enhanced the VEGF-C mRNA and protein expression levels, whereas siRNAs - knocked down MTA1 decreased the VEGF-C expression level.
  
  
  CONCLUSIONS
  MTA1, as a regulator of tumor-associated lymphangiogenesis, promotes lymphangiogenesis in CRC by mediating the VEGF-C expression.
authors: Department of Pathology, Medical School of Jinan University, Guangzhou 510632, Guangdong Province, China.
authors: Bin
authors: B
authors: Du
authors: Zhen-Yu
authors: ZY
authors: Yang
authors: Xue-Yun
authors: XY
authors: Zhong
authors: Mao
authors: M
authors: Fang
authors: Yong-Rong
authors: YR
authors: Yan
authors: Guo-Long
authors: GL
authors: Qi
authors: Yun-Long
authors: YL
authors: Pan
authors: Xu-Long
authors: XL
authors: Zhou
mesh_terms: D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology*; D018450:Disease Progression; D005260:Female; D006655:Histone Deacetylases / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D042583:Lymphangiogenesis / Q000502:physiology*; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D034622:RNA Interference; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D015534:Trans-Activators; D042582:Vascular Endothelial Growth Factor C / Q000378:metabolism*
chemical_list: C089320:MTA1 protein, human; D012097:Repressor Proteins; D015534:Trans-Activators; D042582:Vascular Endothelial Growth Factor C; D006655:Histone Deacetylases