title: [Effect of thermo-chemo therapy on human small cell lung cancer H446 cell apoptosis].
abstract: OBJECTIVE
  To study the synergistic effect of thermo-chemo therapy on lung cancer cells and its possible mechanisms.
  
  
  METHODS
  Refer the normal clinic dose, and use the untreated H446 cells as the control group. H446 cells were treated by different thermo-chemo therapy strategies: 43 degrees C + Paclitaxel (120 microg/L) (thermo-chemotherapy group), 43 degrees C + Paclitaxel (120 microg/L) + NAC (30 micromol/L, specific reactive oxygen species (ROS) inhibitor) (NAC group), and Paclitaxel (120 microg/L) alone (only chemotherapy group). Cell apoptosis was analyzed by FCM and fluorescence was used to measure ROS inside the cells. The expressions of Caspase-3 was determined by Westerm Blotting. Use SPSS 13.0 to perform the statistical analysis on the data.
  
  
  RESULTS
  The rate of cell apoptosis in the thermo-chemo therapy group was significantly higher than those in the other groups (P < 0.05). The ROS in the thermo-chemo therapy group increased (P < 0.05), but NAC inhibited its expression. The expression of Caspase-3 in the thermo-chemo therapy group increased significantly (P < 0.05), but can be inhibited by NAC (P < 0.05).
  
  
  CONCLUSIONS
  Thermo-chemo therapy can significantly seduce the apotosis of H446 cells, probably through seducing ROS forming, realised by the caspase channel.
authors: Department of Radiotherapy, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China. darwomen@yahoo.com.cn
authors: Lin
authors: L
authors: Wang
authors: Xinkui
authors: X
authors: Liu
authors: Shan
authors: S
authors: Gao
authors: Yongjun
authors: Y
authors: Wu
authors: Yiming
authors: Y
authors: Wu
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D017209:Apoptosis / Q000502:physiology*; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D003131:Combined Modality Therapy; D006801:Humans; D006979:Hyperthermia, Induced; D008175:Lung Neoplasms / Q000473:pathology*; D017239:Paclitaxel / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D055752:Small Cell Lung Carcinoma / Q000473:pathology*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D017382:Reactive Oxygen Species; C505407:CASP3 protein, human; D053148:Caspase 3; D017239:Paclitaxel