title: MicroRNA miR-125b induces senescence in human melanoma cells.
abstract: MicroRNAs (miRNAs) are small noncoding RNA molecules involved in gene regulation. Aberrant expression of miRNA has been associated with the development or progression of several diseases, including cancer. In a previous study, we found that the expression of miRNA-125b (miR-125b) was two-fold lower in malignant melanoma producing lymph node micrometastases than in nonmetastasizing tumors. To get further insight into the functional role of miR-125b, we assessed whether its overexpression or silencing affects apoptosis, proliferation, or senescence in melanoma cell lines. We showed that overexpression of miR-125b induced typical senescent cell morphology, including increased cytoplasmatic/nucleus ratio and intensive cytoplasmatic β-galactosidase expression. In contrast, inhibition of miR-125b resulted in 30-35% decreased levels of spontaneous apoptosis. We propose that downregulation of miR-125b in an early cutaneous malignant melanoma can contribute to the increased metastatic capability of this tumor.
authors: Department of Dermatology, Bispebjerg Hospital, Denmark. glud.martin@gmail.com
authors: Martin
authors: M
authors: Glud
authors: Valentina
authors: V
authors: Manfé
authors: Edyta
authors: E
authors: Biskup
authors: Line
authors: L
authors: Holst
authors: Anne Marie Ahlburg
authors: AM
authors: Dirksen
authors: Nina
authors: N
authors: Hastrup
authors: Finn C
authors: FC
authors: Nielsen
authors: Krzysztof T
authors: KT
authors: Drzewiecki
authors: Robert
authors: R
authors: Gniadecki
mesh_terms: D014408:Biomarkers, Tumor / Q000096:biosynthesis / Q000235:genetics; D045744:Cell Line, Tumor; D015536:Down-Regulation; D006801:Humans; D008545:Melanoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000378:metabolism; D058727:RNA, Small Untranslated / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012878:Skin Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D014162:Transfection
chemical_list: D014408:Biomarkers, Tumor; C531888:MIRN125 microRNA, human; D035683:MicroRNAs; D058727:RNA, Small Untranslated