title: Effect of solar eclipse on microbes.
abstract: OBJECTIVE
  A solar eclipse was observed in India on 15(th) January, 2010. It was a total eclipse in some parts of the country, while it was a partial eclipse in other parts. Microorganisms play an important role in various phenomena on the earth. This study was undertaken to know the influence of solar eclipse on nature indirectly, by analyzing certain genotypic and phenotypic variations in prokaryotes and eukaryotes. Since yeast have similar gene expression as that of humans, investigations were pursued on Candida albicans. Hence the study of the effect of solar eclipse on cultures of Staphylococcus aureus, Klebsiella species, Escherichia coli,and C. albicans was performed in the laboratory. The effect of the total or partial eclipse on the microorganism isolated from clinical isolates was investigated during the time period from 11.15 am to 3.15 pm.
  
  
  METHODS
  Cultures of S. aureus, Klebsiella species, and E. coli colonies on nutrient agar slants and broth and C. albicans on Sabouraud's dextrose agar plates and broth. Slants were exposed to sunlight during eclipse and exposure to normal sunlight at Mangalore, Dakshina Kannada district, Karnataka state, India.
  
  
  RESULTS
  There was significant change observed during exposure to normal sunlight and eclipse phase. Bacterial colonies showed difference in morphology on smear examination and sensitivity pattern during this study. One fungal species and three bacterial isolates were studied and changes were recorded. Fungal species showed a definite change in their morphology on exposure to sunlight during eclipse observed by stained smear examination from broth, plate, and slant.
  
  
  CONCLUSIONS
  Present study concludes that blocking of the sun rays during eclipse does not harm prokaryotes and eukaryotes, instead promoted the progeny of predators in the race of better acclimatization and survival in the natural and changing environmental conditions.
authors: Department of Microbiology, A. J. Institute of Medical Sciences, Kuntikan, NH-17, Mangalore -575 004, Karnataka, India.
authors: Amrita
authors: A
authors: Shriyan
authors: Angri M
authors: AM
authors: Bhat
authors: Narendra
authors: N
authors: Nayak