title: The parallel G-quadruplex structure of vertebrate telomeric repeat sequences is not the preferred folding topology under physiological conditions.
abstract: G-quadruplex topologies of telomeric repeat sequences from vertebrates were investigated in the presence of molecular crowding (MC) mimetics, namely polyethylene glycol 200 (PEG), Ficoll 70 as well as Xenopus laevis egg extract by CD and NMR spectroscopy and native PAGE. Here, we show that the conformational behavior of the telomeric repeats in X. laevis egg extract or in Ficoll is notably different from that observed in the presence of PEG. While the behavior of the telomeric repeat in X. laevis egg extract or in Ficoll resembles results obtained under dilute conditions, PEG promotes the formation of high-order parallel topologies. Our data suggest that PEG should not be used as a MC mimetic.
authors: Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University, Max-von-Laue Str. 9, 60438 Frankfurt/Main, Germany.
authors: Robert
authors: R
authors: Hänsel
authors: Frank
authors: F
authors: Löhr
authors: Silvie
authors: S
authors: Foldynová-Trantírková
authors: Ernst
authors: E
authors: Bamberg
authors: Lukás
authors: L
authors: Trantírek
authors: Volker
authors: V
authors: Dötsch
mesh_terms: D000818:Animals; D054856:G-Quadruplexes / Q000187:drug effects; D011092:Polyethylene Glycols / Q000737:chemistry; D011166:Porphyrins / Q000494:pharmacology; D012091:Repetitive Sequences, Nucleic Acid; D016615:Telomere / Q000737:chemistry*; D014982:Xenopus laevis
chemical_list: D011166:Porphyrins; C021096:tetra(4-N-methylpyridyl)porphine; D011092:Polyethylene Glycols