title: CXC chemokine receptor 1 enhances the ability of human umbilical cord blood-derived mesenchymal stem cells to migrate toward gliomas.
abstract: In this study, we showed that knocking-down interleukin-8 (IL-8) in glioma cells, or its receptor, CXC chemokine receptor 1 (CXCR1) in hUCB-MSCs reduced hUCB-MSC migration toward glioma cells in a Transwell chamber. In contrast, CXCR1-transfected hUCB-MSCs (CXCR1-MSCs) showed a superior capacity to migrate toward glioma cells in a Transwell chamber compared to primary hUCB-MSCs. Furthermore, these transfected cells also demonstrated the same ability to migrate toward tumors in mice bearing intracranial human gliomas as shown by histological and in vivo imaging analysis. Our findings indicate that overexpression of CXCR1 could be a useful tool for MSC-based gene therapy to achieve a sufficient quantity of therapeutic MSCs that are localized within tumors.
authors: Department of Neurosurgery, Seoul St. Mary's Hospital, The Catholic University of Korea, Seoul, South Korea.
authors: Seong Muk
authors: SM
authors: Kim
authors: Dal-Soo
authors: DS
authors: Kim
authors: Chang Hyun
authors: CH
authors: Jeong
authors: Dong Hyun
authors: DH
authors: Kim
authors: Ji Hyun
authors: JH
authors: Kim
authors: Hong Bae
authors: HB
authors: Jeon
authors: Soon-Jae
authors: SJ
authors: Kwon
authors: Sin-Soo
authors: SS
authors: Jeun
authors: Yoon Sun
authors: YS
authors: Yang
authors: Wonil
authors: W
authors: Oh
authors: Jong Wook
authors: JW
authors: Chang
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000628:therapy*; D045744:Cell Line, Tumor; D002465:Cell Movement; D005312:Fetal Blood / Q000166:cytology; D055785:Gene Knockdown Techniques; D015316:Genetic Therapy / Q000379:methods*; D005910:Glioma / Q000628:therapy*; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D008819:Mice, Nude; D023062:Receptors, Interleukin-8A / Q000235:genetics*; D014162:Transfection; D023041:Xenograft Model Antitumor Assays
chemical_list: D016209:Interleukin-8; D023062:Receptors, Interleukin-8A