title: Hts/Adducin controls synaptic elaboration and elimination.
abstract: Neural development requires both synapse elaboration and elimination, yet relatively little is known about how these opposing activities are coordinated. Here, we provide evidence Hts/Adducin can serve this function. We show that Drosophila Hts/Adducin is enriched both pre- and postsynaptically at the NMJ. We then demonstrate that presynaptic Hts/Adducin is necessary and sufficient to control two opposing processes associated with synapse remodeling: (1) synapse stabilization as determined by light level and ultrastructural and electrophysiological assays and (2) the elaboration of actin-based, filopodia-like protrusions that drive synaptogenesis and growth. Synapse remodeling is sensitive to Hts/Adducin levels, and we provide evidence that the synaptic localization of Hts/Adducin is controlled via phosphorylation. Mechanistically, Drosophila Hts/Adducin protein has actin-capping activity. We propose that phosphorylation-dependent regulation of Hts/Adducin controls the level, localization, and activity of Hts/Adducin, influencing actin-based synapse elaboration and spectrin-based synapse stabilization. Hts/Adducin may define a mechanism to switch between synapse stability and dynamics.
authors: Department of Biochemistry and Biophysics, University of California, San Francisco, 1550 4(th) Street, San Francisco, CA 94158, USA. jan.pielage@fmi.ch
authors: Jan
authors: J
authors: Pielage
authors: Victoria
authors: V
authors: Bulat
authors: J Bradley
authors: JB
authors: Zuchero
authors: Richard D
authors: RD
authors: Fetter
authors: Graeme W
authors: GW
authors: Davis
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D030801:Animals, Genetically Modified; D001692:Biological Transport / Q000502:physiology; D015153:Blotting, Western; D002148:Calmodulin-Binding Proteins / Q000378:metabolism*; D003599:Cytoskeleton / Q000378:metabolism* / Q000648:ultrastructure; D004330:Drosophila; D029721:Drosophila Proteins / Q000378:metabolism*; D004594:Electrophysiology; D007150:Immunohistochemistry; D046529:Microscopy, Electron, Transmission; D010766:Phosphorylation / Q000502:physiology; D013049:Spectrin / Q000378:metabolism; D013569:Synapses / Q000378:metabolism* / Q000648:ultrastructure; D009435:Synaptic Transmission / Q000502:physiology
chemical_list: D000199:Actins; D002148:Calmodulin-Binding Proteins; D029721:Drosophila Proteins; C108208:hts protein, Drosophila; D013049:Spectrin