title: Sulodexide reduces senescence-related changes in human endothelial cells.
abstract: BACKGROUND
  Senescent endothelial cells acquire functional properties that make the vascular wall more prone to atherosclerotic changes. We tested whether senescence of the endothelial cells maintained in in vitro culture can be moderated by their simultaneous exposure to sulodexide.
  
  
  METHODS
  Replicative aging of the endothelial cells was studied during their 15 passages performed every 4 days in cells cultured in standard medium or in medium supplemented with sulodexide 0.5 LRU/mL. Changes in population doubling time and beta-galactosidase activity were used as indexes of aging and compared with other cellular parameters.
  
  
  RESULTS
  Repeated passages of endothelial cells induce their senescence, as reflected by prolongation of the population doubling time, increased beta-galactosidase activity, oxidative stress and release of cytokines. Healing of the injured endothelial monolayer is impaired in senescent cells. Sulodexide partially prevents oxidative stress and totally eliminates other senescence-related changes such as increased release of MCP-1, lengthening of the population doubling time, and impaired healing of the cellular monolayer after its mechanical injury.
  
  
  CONCLUSIONS
  Sulodexide prevented cellular senescence in cultured endothelial cells, moderating features of the cellular senescence in endothelial cells in in vitro conditions, which potentially may have practical application. The administration of sulodexide could potentially be used in prevention of atherosclerotic changes.
authors: Department of Pathophysiology, Poznan University of Medical Sciences, Poznan, Poland.
authors: Katarzyna
authors: K
authors: Suminska-Jasinska
authors: Alicja
authors: A
authors: Polubinska
authors: Marta
authors: M
authors: Ciszewicz
authors: Adam
authors: A
authors: Mikstacki
authors: Artur
authors: A
authors: Antoniewicz
authors: Andrzej
authors: A
authors: Breborowicz
mesh_terms: D016922:Cellular Senescence / Q000187:drug effects*; D042783:Endothelial Cells / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D005609:Free Radicals / Q000378:metabolism; D006025:Glycosaminoglycans / Q000494:pharmacology*; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors
chemical_list: D005609:Free Radicals; D006025:Glycosaminoglycans; D015850:Interleukin-6; C007858:glucuronyl glucosamine glycan sulfate