title: Cell death induced by the application of alternating magnetic fields to nanoparticle-loaded dendritic cells.
abstract: In this work, the capability of primary, monocyte-derived dendritic cells (DCs) to uptake iron oxide magnetic nanoparticles (MNPs) is assessed and a strategy to induce selective cell death in these MNP-loaded DCs using external alternating magnetic fields (AMFs) is reported. No significant decrease in the cell viability of MNP-loaded DCs, compared to the control samples, was observed after five days of culture. The number of MNPs incorporated into the cytoplasm was measured by magnetometry, which confirmed that 1-5 pg of the particles were uploaded per cell. The intracellular distribution of these MNPs, assessed by transmission electron microscopy, was found to be primarily inside the endosomic structures. These cells were then subjected to an AMF for 30 min and the viability of the blank DCs (i.e. without MNPs), which were used as control samples, remained essentially unaffected. However, a remarkable decrease of viability from approximately 90% to 2-5% of DCs previously loaded with MNPs was observed after the same 30 min exposure to an AMF. The same results were obtained using MNPs having either positive (NH(2)(+)) or negative (COOH(-)) surface functional groups. In spite of the massive cell death induced by application of AMF to MNP-loaded DCs, the number of incorporated magnetic particles did not raise the temperature of the cell culture. Clear morphological changes at the cell structure after magnetic field application were observed using scanning electron microscopy. Therefore, local damage produced by the MNPs could be the main mechanism for the selective cell death of MNP-loaded DCs under an AMF. Based on the ability of these cells to evade the reticuloendothelial system, these complexes combined with an AMF should be considered as a potentially powerful tool for tumour therapy.
authors: Instituto de Nanociencia de Aragón (INA), Zaragoza, Spain.
authors: I
authors: I
authors: Marcos-Campos
authors: L
authors: L
authors: Asín
authors: T E
authors: TE
authors: Torres
authors: C
authors: C
authors: Marquina
authors: A
authors: A
authors: Tres
authors: M R
authors: MR
authors: Ibarra
authors: G F
authors: GF
authors: Goya
mesh_terms: D000042:Absorption; D000954:Antigens, Surface / Q000378:metabolism; D016923:Cell Death; D002454:Cell Differentiation; D002470:Cell Survival; D002478:Cells, Cultured; D003102:Colloids; D003713:Dendritic Cells / Q000166:cytology* / Q000648:ultrastructure; D004705:Endocytosis; D005434:Flow Cytometry; D006801:Humans; D057446:Hydrodynamics; D007963:Leukocytes, Mononuclear / Q000166:cytology; D008027:Light; D008280:Magnetics / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D012542:Scattering, Radiation; D013696:Temperature; D014343:Trypan Blue / Q000378:metabolism
chemical_list: D000954:Antigens, Surface; D003102:Colloids; D014343:Trypan Blue