title: Phenotypic analysis and differentiation of murine mesenchymal stem cells.
abstract: Mesenchymal stem cells (MSCs) hold great promise as therapeutic tools to treat different types of disease and the use of preclinical animal models is mandatory for the development of novel MSC-based therapies. The mouse is one of the most important species used for preclinical experiments and, by extension, so is the isolation and characterization of murine MSCs. This chapter presents methods for the phenotypic analysis of cultured murine MSCs.
authors: National Institute of Science and Technology for Stem Cells and Cell Therapy, Centro Regional de Hemoterapia de Ribeirão Preto-HCFMRP/Universidade de São Paulo, Ribeirão Preto, SP, Brazil. lindolfo_meirelles@hotmail.com
authors: Lindolfo
authors: L
authors: da Silva Meirelles
authors: Dimas Tadeu
authors: DT
authors: Covas
mesh_terms: D050156:Adipogenesis; D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism; D002453:Cell Cycle; D002454:Cell Differentiation; D003114:Colony-Forming Units Assay; D003116:Color; D004247:DNA / Q000378:metabolism; D005110:Extracellular Space / Q000378:metabolism; D005434:Flow Cytometry; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008903:Minerals / Q000378:metabolism; D010012:Osteogenesis; D010641:Phenotype; D014617:Vacuoles / Q000378:metabolism
chemical_list: D000954:Antigens, Surface; D008903:Minerals; D004247:DNA