title: Inhibition of HIV-1 and M-MLV reverse transcriptases by a major polyphenol (3,4,5 tri-O-galloylquinic acid) present in the leaves of the South African resurrection plant, Myrothamnus flabellifolia.
abstract: A polyphenol-rich extract of the medicinal resurrection plant Myrothamnus flabellifolia was shown to inhibit viral (M-MLV and HIV-1) reverse transcriptases. Fractionation and purification of this extract yielded the major polyphenol, 3,4,5 tri-O-galloylquinic acid, as the main active compound. A sensitive, ethidium bromide based fluorescent assay, was developed and used to monitor the kinetics of M-MLV and HIV-1 reverse transcriptases in the presence and absence of 3,4,5 tri-O-galloylquinic acid. Kinetic monitoring of these enzymes in the presence of 3,4,5 tri-O-galloylquinic acid revealed non-competitive inhibition with IC(50) values of 5 μM and 34 μM for the M-MLV and HIV-1 enzymes, respectively. We propose that 3,4,5 tri-O-galloylquinic acid and related polymers have potential as indigenous drugs for anti-viral therapy.
authors: Department of Molecular and Cell Biology, University of Cape Town, Rondebosch 7701, South Africa.
authors: Arox
authors: A
authors: Kamng'ona
authors: John P
authors: JP
authors: Moore
authors: George
authors: G
authors: Lindsey
authors: Wolf
authors: W
authors: Brandt
mesh_terms: D004305:Dose-Response Relationship, Drug; D005707:Gallic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D015497:HIV-1 / Q000201:enzymology*; D019684:Magnoliopsida / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D008979:Moloney murine leukemia virus / Q000201:enzymology*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D011801:Quinic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism*; D018894:Reverse Transcriptase Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D013019:South Africa; D013329:Structure-Activity Relationship
chemical_list: D010936:Plant Extracts; D018894:Reverse Transcriptase Inhibitors; D011801:Quinic Acid; D005707:Gallic Acid; C061995:3,4,5-tri-O-galloylquinic acid; D012194:RNA-Directed DNA Polymerase