title: Pharmacological studies of Cav3.1 T-type calcium channels using automated patch-clamp techniques.
abstract: T-type calcium channels are involved in a variety of physiological and pathophysiological processes, and thus could be therapeutic targets. However, there is no T-type channel selective blocker for use in clinical practice, demanding a need for the development of novel drugs where a higher-throughput screening system is required. Here we present pharmacological studies on Ca(v)3.1 T-type channels using automated patch-clamp. The IC(50) values obtained from automated patch-clamp and conventional one showed a good correlation (correlation coefficient of 0.82), suggesting that the automated patch-clamp is an efficient and reliable method for ranking the drug potencies for T-type channels.
authors: Life/Health Division, Korea Institute of Science and Technology, 39-1 Hawolgok-Dong, Seongbuk-Gu, Seoul 136-791, Korea. keehyun@kist.re.kr
authors: Kee Hyun
authors: KH
authors: Choi
authors: Chiman
authors: C
authors: Song
authors: Chan S
authors: CS
authors: Cheong
authors: Hyewhon
authors: H
authors: Rhim
mesh_terms: D002121:Calcium Channel Blockers / Q000494:pharmacology*; D020747:Calcium Channels, T-Type / Q000187:drug effects / Q000502:physiology*; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D055808:Drug Discovery; D004353:Drug Evaluation, Preclinical; D057809:HEK293 Cells; D006801:Humans; D020128:Inhibitory Concentration 50; D018408:Patch-Clamp Techniques / Q000379:methods*
chemical_list: D002121:Calcium Channel Blockers; D020747:Calcium Channels, T-Type