title: Oligodendroglial cells express and secrete reelin.
abstract: Oligodendrocyte (OL) progenitor cells (OPCs) give rise to the myelinating cells of the central nervous system (CNS), the OL. To examine molecular changes involved in OPC differentiation, a microarray analysis was performed at several time points during OPC maturation. The results revealed significant expression levels of mRNA for reelin, one reelin receptor, very low density lipoprotein receptor (Vldlr), and the cytoplasmic adaptor molecule, disabled homolog 1 (Dab1). The expression of these proteins in oligodendroglial (ODG) cells was confirmed by immunocytochemistry and Western blot analysis. It was also discovered that both progenitors and mature OLs secrete reelin. Although there is no known effect of reelin on ODG cells, the data suggest that these cells may be a source of reelin in the CNS.
authors: Department of Cell and Developmental Biology, State University of New York Upstate Medical University, Syracuse, 13210, USA.
authors: Justin R
authors: JR
authors: Siebert
authors: Donna J
authors: DJ
authors: Osterhout
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D015153:Blotting, Western; D015816:Cell Adhesion Molecules, Neuronal / Q000378:metabolism*; D002454:Cell Differentiation; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D007124:Immunoenzyme Techniques; D047468:Immunoprecipitation; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D009457:Neuroglia / Q000378:metabolism*; D009836:Oligodendroglia / Q000166:cytology / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D011956:Receptors, Cell Surface / Q000378:metabolism; D011973:Receptors, LDL / Q000235:genetics / Q000378:metabolism; D000091042:Reelin Protein; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012697:Serine Endopeptidases / Q000378:metabolism*; D013234:Stem Cells / Q000378:metabolism*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D015415:Biomarkers; D015816:Cell Adhesion Molecules, Neuronal; C499786:Dab1 protein, rat; D016326:Extracellular Matrix Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D011973:Receptors, LDL; D000091042:Reelin Protein; C000717129:Reln protein, rat; C036053:VLDL receptor; C469363:reelin receptor; D012697:Serine Endopeptidases