title: Detection of antigliadin autoantibodies in celiac patient samples using a cyclodextrin-based supramolecular biosensor.
abstract: Celiac disease is a condition associated with the ingestion of gluten by genetically susceptible individuals. Measurement of serum antigliadin antibodies is a diagnostic tool also used as a means of monitoring a patient's compliance to a gluten-free diet. In this work, we demonstrate the applicability of an electrochemical supramolecular platform based on cyclodextrin-modified gold surfaces to detect antigliadin antibodies in real serum samples. Several support layer-biorecognition element combinations were tested in order to maximize the electrochemical response, and the assay was optimized in terms of incubation times and resistance to nonspecific interactions. The developed supramolecular biosensor was then applied to the amperometric detection of antigliadin IgA and IgG autoantibodies in real samples of celiac disease patients under follow-up treatment; the results were compared with a commercial enzyme linked immunosorbent assay (ELISA) test, and an excellent correlation was observed between both methods.
authors: Nanobiotechnology and Bioanalysis Group, Departament d'Enginyeria Química, Universitat Rovira i Virgili, Avinguda Països Catalans 26, 43007 Tarragona, Spain.
authors: Mayreli
authors: M
authors: Ortiz
authors: Alex
authors: A
authors: Fragoso
authors: Ciara K
authors: CK
authors: O'Sullivan
mesh_terms: D001323:Autoantibodies / Q000097:blood*; D015374:Biosensing Techniques; D002446:Celiac Disease / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D005903:Gliadin / Q000097:blood / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D006801:Humans; D046911:Macromolecular Substances / Q000737:chemistry; D008968:Molecular Conformation; D013499:Surface Properties; D047392:beta-Cyclodextrins / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D001323:Autoantibodies; D046911:Macromolecular Substances; D047392:beta-Cyclodextrins; D006046:Gold; D005903:Gliadin; C031215:betadex