title: Synthesis, characterization and cytocompatibility studies of α-chitin hydrogel/nano hydroxyapatite composite scaffolds.
abstract: α-chitin hydrogel/nano hydroxyapatite (nHAp) composite scaffold have been synthesized by freeze-drying approach with nHAp and α-chitin hydrogel. The prepared nHAp and nanocomposite scaffolds were characterized using DLS, SEM, FT-IR, XRD and TGA studies. The porosity, swelling, degradation, protein adsorption and biomineralization (calcification) of the prepared nanocomposite scaffolds were evaluated. Cell viability, attachment and proliferation were investigated using MG 63, Vero, NIH 3T3 and nHDF cells to confirm that the nanocomposite scaffolds were cytocompatible and cells were found to attach and spread on the scaffolds. All the results suggested that these scaffolds can be used for bone and wound tissue engineering.
authors: Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham University, Kochi 682041, India.
authors: P T Sudheesh
authors: PT
authors: Kumar
authors: Sowmya
authors: S
authors: Srinivasan
authors: Vinoth-Kumar
authors: VK
authors: Lakshmanan
authors: H
authors: H
authors: Tamura
authors: S V
authors: SV
authors: Nair
authors: R
authors: R
authors: Jayakumar
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D049109:Cell Proliferation; D002470:Cell Survival; D002686:Chitin / Q000737:chemistry*; D002522:Chlorocebus aethiops; D017886:Durapatite / Q000737:chemistry*; D005612:Freeze Drying; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000138:chemical synthesis* / Q000737:chemistry; D051379:Mice; D008855:Microscopy, Electron, Scanning; D041681:NIH 3T3 Cells; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014709:Vero Cells; D014945:Wound Healing; D014961:X-Ray Diffraction
chemical_list: D002686:Chitin; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D017886:Durapatite