title: Osteogenic differentiation of human multipotent mesenchymal stromal cells.
abstract: A comprehensive knowledge of the molecular biology underlying osteogenic differentiation in a controlled, laboratory setting may promise optimization of future cell-based tissue engineering strategies for clinical problems. The scope of this review encompasses a discussion of the methodology utilized to perform such studies. Our laboratory routinely performs both in vitro and in vivo assays underlying osteogenic differentiation, and the widespread use of singular methodology across multiple investigators and institutions promises great advancements for the skeletal tissue engineering community.
authors: Hagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford University School of Medicine, Stanford, CA, USA.
authors: Deepak M
authors: DM
authors: Gupta
authors: Nicholas J
authors: NJ
authors: Panetta
authors: Michael T
authors: MT
authors: Longaker
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000880:Anthraquinones / Q000378:metabolism; D001031:Apatites / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology; D002452:Cell Count; D002454:Cell Differentiation; D002469:Cell Separation; D064987:Cell- and Tissue-Based Therapy; D015925:Cryopreservation; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D010012:Osteogenesis; D013194:Staining and Labeling
chemical_list: D000880:Anthraquinones; D001031:Apatites; C010078:alizarin; D000469:Alkaline Phosphatase