title: Biofunctionalization of silicone polymers using poly(amidoamine) dendrimers and a mannose derivative for prolonged interference against pathogen colonization.
abstract: Despite numerous preventive strategies on bacterial adhesion, pathogenic biofilm formation remained the major cause of medical device-related infections. Bacterial interference is a promising strategy that uses pre-established biofilms of benign bacteria to serve as live, protective coating against pathogen colonization. However, the application of this strategy to silicone urinary catheters was hampered by low adherence of benign bacteria onto silicone materials. In this work, we present a general method for biofunctionalization of silicone (PDMS) as one of the most widely used materials for biomedical devices. We used mild CO(2) plasma to activate PDMS surface followed by simple attachment of generation 5 (G5) poly(amidoamine) (PAMAM) dendrimers to generate an amino-terminated surface that were maintained even after storage in PBS buffer for 36 days. We then covalently attach a carboxy-terminated mannose derivative to the modified PDMS to promote the adherence of benign Escherichia coli 83972 expressing mannose-binding type 1 fimbriae. We demonstrated that dense, stable biofilms of E. coli 83972 could be established within 48 h on the mannose-coated PDMS. Significantly, this benign biofilm reduced the adherence of the uropathogenic Enterococcus faecalis by 104-fold after 72 h, while the benign bacteria on the unmodified substrate by only 5.5-fold.
authors: Department of Chemistry, University of Houston, TX 77204, USA.
authors: Analette I
authors: AI
authors: Lopez
authors: Amit
authors: A
authors: Kumar
authors: Megan R
authors: MR
authors: Planas
authors: Yan
authors: Y
authors: Li
authors: Thuy V
authors: TV
authors: Nguyen
authors: Chengzhi
authors: C
authors: Cai
mesh_terms: D000588:Amines / Q000737:chemistry*; D000898:Antibiosis; D001422:Bacterial Adhesion; D018441:Biofilms; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000378:metabolism; D050091:Dendrimers / Q000737:chemistry*; D013293:Enterococcus faecalis / Q000472:pathogenicity; D004926:Escherichia coli / Q000502:physiology; D006801:Humans; D008358:Mannose / Q000737:chemistry*; D008422:Materials Testing; D015394:Molecular Structure; D011108:Polymers / Q000737:chemistry*; D012828:Silicones / Q000737:chemistry*; D013499:Surface Properties; D014546:Urinary Catheterization / Q000009:adverse effects; D014552:Urinary Tract Infections / Q000209:etiology / Q000382:microbiology
chemical_list: D000588:Amines; D020099:Coated Materials, Biocompatible; D050091:Dendrimers; D011108:Polymers; D012828:Silicones; D008358:Mannose