title: Immune response to Moloney-murine leukemia virus-induced antigens in bone marrow.
abstract: By exploring induction and persistence of virus-specific memory CD8(+) T cells in the BM of Moloney-murine sarcoma/leukemia virus-immune mice, we observed that the amount of activated CD8(+)CD62L(-) cells increased more rapidly and persisted for a longer period than in peripheral organs. Among the CD8(+)CD62L(-) subset, the few cells, specific for M-MuLV encoded antigens, expressing TCRVβ5 rearrangements increased in an explosive manner doubling the percentage of TCRVβ5(+) subset so that as a final result more than 10% of CD8(+) lymphocytes became potential virus-specific cytotoxic effectors. The numerical expansion of Vβ5(+) cells started and persisted in the same proportion among both CD8(+)CD62L(-) and CD8(+)CD62L(+) subsets. In these subsets the analysis of CD44 phenotype, to distinguish effector (TEM) and central (TCM) memory, evidenced a twofold increase of Vβ5(+) TEM percentage and fourfold increase of Vβ5(+) TCM. In parallel, the non virus-specific Vβ5(-) counterpart, also numerically increased due to the CD8(+) expansion, was partially reduced as TEM percentage and doubled as TCM percentage. We conclude that the immune response to M-MuLV encoded antigens in BM generate not only a large number of virus-specific memory cells but also the re-shaping of the entire memory T cell repertoire.
authors: Department of Molecular Pathology, Faculty of Medicine, Polytechnic University of Marche, Ancona, Italy. g.biasi@unipd.it
authors: Giovanni
authors: G
authors: Biasi
authors: Antonella
authors: A
authors: Facchinetti
authors: Roberta
authors: R
authors: Cappellari
authors: Elisabetta
authors: E
authors: Rossi
authors: Paola
authors: P
authors: Zanovello
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000276:immunology*; D001853:Bone Marrow / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D000939:Epitopes / Q000276:immunology; D007114:Immunization; D007156:Immunologic Memory / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008979:Moloney murine leukemia virus / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
chemical_list: D000956:Antigens, Viral; D000939:Epitopes