title: Carboxyl-functionalized superparamagnetic Fe3O4/poly(St-co-MPS)/SiO2 composite particles for rapid and sensitive immunoassay.
abstract: Superparamagnetic Fe3O4/poly(St-co-MPS)/SiO2 composite particles with the average size of 140 nm were functionalized with carboxyl group by emulsion polymerization. Functionalized particles with carboxyl contents of 13.6 and 136 micromol/mL were prepared by changing the amount of acrylic acid monomer used in the polymerization. After conjugation with human chorionic gonadotrophin (hCG) antibody, the particles were used to construct lateral flow immunoassays (LFIA) for the detection of hCG in solution. The quantitative analysis could be finished in 20 min by using the magnetic particles as labels. The detection limit of LFIA was determined to be 1 and 5 IU/L respectively for the magnetic particles with carboxyl contents of 13.6 and 136 micromol/mL.
authors: State Key Laboratory for Supramolecular Structures and Materials, College of Chemistry, Jilin University, Changchun 130012, PR China.
authors: Hang
authors: H
authors: Yuan
authors: Feng
authors: F
authors: Yan
authors: Lan
authors: L
authors: Ma
authors: Feng
authors: F
authors: Wu
authors: Jiaqi
authors: J
authors: Zhuang
authors: Wensheng
authors: W
authors: Yang
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D006063:Chorionic Gonadotropin / Q000032:analysis* / Q000276:immunology; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005290:Ferric Compounds / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation*; D008280:Magnetics / Q000295:instrumentation*; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D006063:Chorionic Gonadotropin; D005290:Ferric Compounds; C000499:ferric oxide; D012822:Silicon Dioxide