title: Visualization of neuropeptides in paraffin-embedded tissue sections of the central nervous system in the decapod crustacean, Penaeus monodon, by imaging mass spectrometry.
abstract: The distributions of neuropeptides in paraffin-embedded tissue sections (PETS) of the eyestalk, brain, and thoracic ganglia of the shrimp Penaeus monodon were visualized by imaging mass spectrometry (IMS). Peptide signals were obtained from PETS without affecting morphological features. Twenty-nine neuropeptides comprising members of FMRFamide, SIFamides, crustacean hyperglycaemic hormone, orcokinin-related peptides, tachykinin-related peptides, and allatostatin A were detected and visualized. Among these findings we first identified tachykinin-related peptide as a novel neuropeptide in this shrimp species. We found that these neuropeptides were distributed at specific areas in the three neural organs. In addition, 28 peptide sequences derived from 4 types of constitutive proteins, including actin, histones, arginine kinase, and cyclophilin A were also detected. All peptide sequences were verified by liquid chromatography-tandem mass spectrometry. The use of IMS on acetic acid-treated PETS enabled us to identify peptides and obtain their specific localizations in correlation with the undisturbed histological structure of the tissue samples.
authors: Department of Anatomy, Mahidol University, Ratchathewi, Bangkok, Thailand. piyachatnet@gmail.com
authors: Piyachat
authors: P
authors: Chansela
authors: Naoko
authors: N
authors: Goto-Inoue
authors: Nobuhiro
authors: N
authors: Zaima
authors: Morakot
authors: M
authors: Sroyraya
authors: Prasert
authors: P
authors: Sobhon
authors: Mitsutoshi
authors: M
authors: Setou
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism; D002490:Central Nervous System / Q000378:metabolism*; D013058:Mass Spectrometry / Q000379:methods*; D009479:Neuropeptides / Q000737:chemistry / Q000378:metabolism*; D010232:Paraffin / Q000737:chemistry; D033561:Penaeidae / Q000378:metabolism*
chemical_list: D009479:Neuropeptides; D010232:Paraffin