title: Bubble-free on-chip continuous-flow polymerase chain reaction: concept and application.
abstract: Bubble formation inside a microscale channel is a significant problem in general microfluidic experiments. The problem becomes especially crucial when performing a polymerase chain reaction (PCR) on a chip which is subject to repetitive temperature changes. In this paper, we propose a bubble-free sample injection scheme applicable for continuous-flow PCR inside a glass/PDMS hybrid microfluidic chip, and attempt to provide a theoretical basis concerning bubble formation and elimination. Highly viscous paraffin oil plugs are employed in both the anterior and posterior ends of a sample plug, completely encapsulating the sample and eliminating possible nucleation sites for bubbles. In this way, internal channel pressure is increased, and vaporization of the sample is prevented, suppressing bubble formation. Use of an oil plug in the posterior end of the sample plug aids in maintaining a stable flow of a sample at a constant rate inside a heated microchannel throughout the entire reaction, as compared to using an air plug. By adopting the proposed sample injection scheme, we demonstrate various practical applications. On-chip continuous-flow PCR is performed employing genomic DNA extracted from a clinical single hair root sample, and its D1S80 locus is successfully amplified. Also, chip reusability is assessed using a plasmid vector. A single chip is used up to 10 times repeatedly without being destroyed, maintaining almost equal intensities of the resulting amplicons after each run, ensuring the reliability and reproducibility of the proposed sample injection scheme. In addition, the use of a commercially-available and highly cost-effective hot plate as a potential candidate for the heating source is investigated.
authors: Gachon BioNano Research Institute, Kyungwon University, San 65 Bokjeong-dong, Sujeong-gu, Seongnam, Gyeonggi-do 461-701, Korea.
authors: Wenming
authors: W
authors: Wu
authors: Kyung-Tae
authors: KT
authors: Kang
authors: Nae Yoon
authors: NY
authors: Lee
mesh_terms: D004247:DNA / Q000032:analysis; D018859:Hair Follicle / Q000378:metabolism; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods; D009821:Oils / Q000737:chemistry; D010232:Paraffin / Q000737:chemistry; D016133:Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods*; D013696:Temperature
chemical_list: D009821:Oils; D010232:Paraffin; C015418:paraffin oils; D004247:DNA