title: Secreted factors from brain endothelial cells maintain glioblastoma stem-like cell expansion through the mTOR pathway.
abstract: Glioma stem-cells are associated with the brain vasculature. However, the way in which this vascular niche regulates stem-cell renewal and fate remains unclear. Here, we show that factors emanating from brain endothelial cells positively control the expansion of long-term glioblastoma stem-like cells. We find that both pharmacological inhibition of and RNA interference with the mammalian target of rapamycin (mTOR) pathway reduce their spheroid growth. Conversely, the endothelial secretome is sufficient to promote this mTOR-dependent survival. Thus, interfering with endothelial signals might present opportunities to identify treatments that selectively target malignant stem-cell niches.
authors: INSERM U1016, Institut Cochin, 22 rue Mechain, 75014 Paris.
authors: Eva Maria
authors: EM
authors: Galan-Moya
authors: Armelle
authors: A
authors: Le Guelte
authors: Evelyne
authors: E
authors: Lima Fernandes
authors: Cécile
authors: C
authors: Thirant
authors: Julie
authors: J
authors: Dwyer
authors: Nicolas
authors: N
authors: Bidere
authors: Pierre-Olivier
authors: PO
authors: Couraud
authors: Mark G H
authors: MG
authors: Scott
authors: Marie-Pierre
authors: MP
authors: Junier
authors: Hervé
authors: H
authors: Chneiweiss
authors: Julie
authors: J
authors: Gavard
mesh_terms: D015153:Blotting, Western; D001921:Brain / Q000098:blood supply / Q000166:cytology*; D042783:Endothelial Cells / Q000378:metabolism*; D005434:Flow Cytometry; D005663:Furans / Q000494:pharmacology; D005909:Glioblastoma / Q000503:physiopathology*; D006801:Humans; D008856:Microscopy, Fluorescence; D011725:Pyridines / Q000494:pharmacology; D011743:Pyrimidines / Q000494:pharmacology; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D015398:Signal Transduction / Q000502:physiology*; D020123:Sirolimus / Q000494:pharmacology; D013234:Stem Cells / Q000378:metabolism* / Q000502:physiology; D058570:TOR Serine-Threonine Kinases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
chemical_list: D005663:Furans; C522973:PI103; D011725:Pyridines; D011743:Pyrimidines; D034741:RNA, Small Interfering; D058570:TOR Serine-Threonine Kinases; D020123:Sirolimus