title: Evaluation of the multipotent character of human foreskin-derived precursor cells.
abstract: In the present study, the trilineage differentiation capacity of human foreskin-derived precursor cells (hSKP) was evaluated upon exposure to various (non)commercial (i and ii) ectodermal, (iii) mesodermal and (iv) endodermal differentiation media. (i) Upon sequential exposure of the cells to keratinocyte growth (CnT-07® or CnT-057®) and differentiation (CnT-02® or Epilife®) media, keratinocyte-like cells (filaggrin(+)/involucrin(+)) were obtained. The preferred keratinocyte differentiation strategy was exposure to CnT-07®. (ii) When hSKP were subsequently exposed to NeuroCult® media, cells underwent a weak neuro-ectodermal differentiation expressing nestin, myelin binding protein (MBP), vimentin and alpha-foetoprotein (AFP). Sequential exposure to NPMM® and NPDM® generated cells with an inferior neuro-ectodermal phenotype (nestin(+)/vimentin(+)/MBP(-)/AFP(-)). (iii) Upon exposure of hSKP to insulin-transferrin-selenite (ITS) and dexamethasone, small lipid droplets were observed, suggesting their differentiation potential towards adipocyte-like cells. (iv) Finally, after sequential exposure to hepatogenic growth factors and cytokines, an immature hepatic cell population was generated. The presence of pre-albumin suggests that a sequential exposure strategy is here superior to a cocktail approach. In summary, a considerable impact of different (non)commercial media on the lineage-specific differentiation efficiency of hSKP is shown. In addition, we demonstrate here for the first time that, in a suitable keratinocyte stimulating micro-environment, hSKP can generate keratinocyte-like progeny in vitro.
authors: Dept. Toxicology, Center for Pharmaceutical Research, Vrije Universiteit Brussel (VUB), Laarbeeklaan 103, B-1090 Brussels, Belgium. jdekock@vub.ac.be
authors: Joery
authors: J
authors: De Kock
authors: Sarah
authors: S
authors: Snykers
authors: Eva
authors: E
authors: Ramboer
authors: Simke
authors: S
authors: Demeester
authors: Anja
authors: A
authors: Heymans
authors: Steven
authors: S
authors: Branson
authors: Tamara
authors: T
authors: Vanhaecke
authors: Vera
authors: V
authors: Rogiers
mesh_terms: D002454:Cell Differentiation; D016207:Cytokines / Q000378:metabolism; D004475:Ectoderm / Q000378:metabolism; D004707:Endoderm / Q000378:metabolism; D000091344:Filaggrin Proteins; D052816:Foreskin / Q000166:cytology*; D022781:Hepatocytes / Q000378:metabolism; D006801:Humans; D007223:Infant; D015603:Keratinocytes / Q000378:metabolism; D008297:Male; D008648:Mesoderm / Q000378:metabolism; D013234:Stem Cells / Q000378:metabolism*
chemical_list: D016207:Cytokines; C000717249:FLG protein, human; D000091344:Filaggrin Proteins