title: Activated and inactivated PPARs-γ modulate experimentally induced colitis in rats.
abstract: BACKGROUND
  This study sought to define the mechanism by which PPAR-γ ligands affect the course of experimentally induced colitis in rats.
  
  
  METHODS
  Inflammation was induced in Wistar rats by a single rectal administration of 2,4,6,-trinitrobenzene sulfonic acid (TNBS). The antagonist of PPARγ antagonist, bisphenol A diglycidyl ether (BADGE), was administrated intraperitoneally 120 mg/kg 4 times every other day. Rosiglitazone 8 mg/kg was administrated by gastric tube 4 times. Body weight was measured daily. After killing, the large intestinal tissue was weighed and collected for histopathologic and immunoenzymatic tests. Levels of IL-6, IL-10, and myeloperoxidase (MPO) were determined in serum and in intestinal homogenates.
  
  
  RESULTS
  Rats receiving rosiglitazone had higher body weight, whereas large intestine weight/length ratio was lower; histology showed fewer inflammatory markers. Rats receiving TNBS and TNBS along with BADGE had more intensive inflammatory changes. Rosiglitazone alone decreased expression of IL-6; used with TNBS it decreased expression of MPO in intestinal tissue, yet did not increase the expression of IL-10. Decreased levels of MPO indicate reduced neutrophil-dependent immune response. The antagonist of PPAR-γ increased IL-6 in serum and decreased IL-10 in intestinal homogenates. Bisphenol A diglycidyl ether administrated to healthy animals increases serum IL-6 levels.
  
  
  CONCLUSIONS
  Rosiglitazone inhibits experimental inflammation; administration of its selective antagonist abolishes this protective influence. Rosiglitazone inhibits expression of proinflammatory IL-6 and does not affect IL-10. Agonists of PPARs-γ are possibilities for inflammatory bowel disease prevention. Exogenous substances blocking PPARs-γ may contribute to development or relapse of nonspecific inflammatory bowel diseases.
authors: Department of Gastroenterology, Medical University of Lublin, Lublin, Poland. celinski@mp.pl
authors: Krzysztof
authors: K
authors: Celinski
authors: Tomasz
authors: T
authors: Dworzanski
authors: Agnieszka
authors: A
authors: Korolczuk
authors: Maria
authors: M
authors: Slomka
authors: Sebastian
authors: S
authors: Radej
authors: Halina
authors: H
authors: Cichoz-Lach
authors: Agnieszka
authors: A
authors: Madro
mesh_terms: D000818:Animals; D001835:Body Weight; D003092:Colitis / Q000097:blood / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D003106:Colon / Q000378:metabolism / Q000473:pathology; D016753:Interleukin-10 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D009929:Organ Size; D047495:PPAR gamma / Q000378:metabolism*; D009195:Peroxidase / Q000097:blood; D051381:Rats; D017208:Rats, Wistar; D014020:Tissue Extracts
chemical_list: D015850:Interleukin-6; D047495:PPAR gamma; D014020:Tissue Extracts; D016753:Interleukin-10; D009195:Peroxidase