title: Subcellular distribution of nitric oxide synthase isoforms in the rat duodenum.
abstract: OBJECTIVE
  To study the cell-type specific subcellular distribution of the three isoforms of nitric oxide synthase (NOS) in the rat duodenum.
  
  
  METHODS
  Postembedding immunoelectronmicroscopy was performed, in which primary antibodies for neuronal NOS (nNOS), endothelial NOS (eNOS), and inducible NOS (iNOS), were visualized with protein A-gold-conjugated secondary antibodies. Stained ultrathin sections were examined and photographed with a Philips CM10 electron microscope equipped with a MEGAVIEW II camera. The specificity of the immunoreaction in all cases was assessed by omitting the primary antibodies in the labeling protocol and incubating the sections only in the protein A-gold conjugated secondary antibodies.
  
  
  RESULTS
  Postembedding immunoelectronmicroscopy revealed the presence of nNOS, eNOS, and iNOS immunoreactivity in the myenteric neurons, the enteric smooth muscle cells, and the endothelium of capillaries running in the vicinity of the myenteric plexus of the rat duodenum. The cell type-specific distributions of the immunogold particles labeling the three different NOS isozymes were revealed. In the control experiments, in which the primary antiserum was omitted, virtually no postembedding gold particles were observed.
  
  
  CONCLUSIONS
  This postembedding immunoelectronmicroscopic study provided the first evidence of cell-type-specific differences in the subcellular distributions of NOS isoforms.
authors: Department of Physiology, Anatomy and Neuroscience, University of Szeged, Hungary.
authors: Petra
authors: P
authors: Talapka
authors: Nikolett
authors: N
authors: Bódi
authors: Izabella
authors: I
authors: Battonyai
authors: Eva
authors: E
authors: Fekete
authors: Mária
authors: M
authors: Bagyánszki
mesh_terms: D000818:Animals; D004386:Duodenum / Q000201:enzymology* / Q000648:ultrastructure*; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000378:metabolism*; D016253:Microscopy, Immunoelectron / Q000295:instrumentation / Q000379:methods; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D051381:Rats
chemical_list: D007527:Isoenzymes; D019001:Nitric Oxide Synthase