title: Identification of viral suppressors of RNAi by a reporter assay in Drosophila S2 cell culture.
abstract: The RNA interference (RNAi) pathway plays an important role in antiviral immunity in insects. To -counteract the RNAi-mediated immune response of their hosts, several insect viruses, such as Flock house virus, Drosophila C virus, and Cricket paralysis virus, encode potent viral suppressors of RNAi (VSRs). Because of the importance of RNAi in antiviral defense in insects, other insect viruses are likely to encode VSRs as well. In this chapter, we describe a detailed protocol for an RNAi reporter assay in Drosophila S2 cells for the identification of VSR activity.
authors: Department of Medical Microbiology, Radboud University Nijmegen Medical Centre, Nijmegen Centre for Molecular Life Sciences, Nijmegen Institute for Infection, Inflammation and Immunity, Nijmegen, The Netherlands.
authors: Koen W R
authors: KW
authors: van Cleef
authors: Joël T
authors: JT
authors: van Mierlo
authors: Marius
authors: M
authors: van den Beek
authors: Ronald P
authors: RP
authors: van Rij
mesh_terms: D000818:Animals; D002460:Cell Line; D004331:Drosophila melanogaster / Q000166:cytology* / Q000235:genetics / Q000821:virology*; D017930:Genes, Reporter / Q000235:genetics*; D016153:Genes, Suppressor; D005814:Genes, Viral / Q000235:genetics*; D049409:Luciferases, Firefly / Q000235:genetics; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000096:biosynthesis / Q000235:genetics; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: D012330:RNA, Double-Stranded; D049409:Luciferases, Firefly