title: Myocardial substrate and route of administration determine acute cardiac retention and lung bio-distribution of cardiosphere-derived cells.
abstract: BACKGROUND
  Quantification of acute myocardial retention and lung bio-distribution of cardiosphere-derived cells (CDCs) following transplantation is important to improve engraftment.
  
  
  RESULTS
  We studied acute(1 hour) cardiac/lung retention in 4 groups (n = 25) of rats (normal--NL, acute ischemia-reperfusion--AI-RM, acute permanent ligation-PL, and chronic infarct by ischemia-reperfusion--CI-R) using intra-myocardial delivery, 1 group using intracoronary delivery (acute ischemia-reperfusion, AI-RC, n = 5) and 1 group using intravenous delivery (acute ischemia-reperfusion, AI-RV, n = 5) of CDCs by PET. Cardiac retention was similar in the NL, AI-RM, CI-R, and A-IRC groups (13.6% ± 2.3% vs. 12.0% ± 3.9% vs. 9.9 ± 2.8 vs. 15.4% ± 5.5%; P = NS), but higher in PL animals (22.9% ± 5.2%; P < .05). Low cardiac retention was associated with significantly higher lung activity in NL and AI-RM groups (43.3% ± 5.6% and 39.9% ± 9.3%), compared to PL (28.5% ± 5.9%), CI-R (20.2% ± 9.3%), and A-IRC (19.9% ± 5.6%) animals (P < .05 vs. AI-RM and NL). Lung activity was highest following intravenous CDC delivery (55.1% ± 9.3%, P < .001) and was associated with very low cardiac retention (0.8% ± 1.06%). Two-photon microscopy indicated that CDCs escaped to the lungs via the coronary veins following intra-myocardial injection.
  
  
  CONCLUSIONS
  Acute cardiac retention and lung bio-distribution vary with the myocardial substrate and injection route. Intra-myocardially injected CDCs escape into the lungs via coronary veins, an effect that is more pronounced in perfused myocardium.
authors: Johns Hopkins University, 720 Rutland Ave, Ross 871, Baltimore, MD 21210, USA.
authors: Michael
authors: M
authors: Bonios
authors: John
authors: J
authors: Terrovitis
authors: Connie Y
authors: CY
authors: Chang
authors: James M
authors: JM
authors: Engles
authors: Takahiro
authors: T
authors: Higuchi
authors: Riikka
authors: R
authors: Lautamäki
authors: Jianhua
authors: J
authors: Yu
authors: James
authors: J
authors: Fox
authors: Martin
authors: M
authors: Pomper
authors: Richard L
authors: RL
authors: Wahl
authors: Benjamin M
authors: BM
authors: Tsui
authors: Brian
authors: B
authors: O'Rourke
authors: Frank M
authors: FM
authors: Bengel
authors: Eduardo
authors: E
authors: Marbán
authors: M Roselle
authors: MR
authors: Abraham
mesh_terms: D000818:Animals; D002460:Cell Line; D005260:Female; D008168:Lung / Q000473:pathology*; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000000981:diagnostic imaging / Q000473:pathology*; D017202:Myocardial Ischemia / Q000000981:diagnostic imaging / Q000473:pathology* / Q000601:surgery*; D009928:Organ Specificity; D011877:Radionuclide Imaging; D051381:Rats; D016896:Treatment Outcome