title: Anti-Helicobacter pylori activity of fermented milk with lactic acid bacteria.
abstract: BACKGROUND
  Ten strains of lactic acid bacteria (LAB) were investigated for their anti-Helicobacter pylori effects. The bactericidal activity and organic acid content in spent culture supernatants (SCS) from fermented milk were measured. In addition, the exclusion effect of SCS against H. pylori infection of human gastric epithelial AGS cells was assayed.
  
  
  RESULTS
  Three LAB strains, LY1, LY5 and IF22, showed better anti-Helicobacter effects than the other strains. There were no significant differences in the bactericidal activity of LAB strains between original SCS, artificial SCS and SCS treated by heating or protease digestion. However, neutralised SCS lost this activity. These results suggest that the anti-H. pylori activity of SCS may be related to the concentration of organic acids and the pH value but not to protein components. In the AGS cell culture test, both fermented LY5-SCS and artificial LY5-SCS significantly reduced H. pylori infection and urease activity (P < 0.05).
  
  
  CONCLUSIONS
  In this study, in vitro methods were used to screen potential probiotics with anti-H. pylori activity. This may provide an excellent and rapid system for studying probiotics in the functional food and dairy industries.
authors: School of Pharmacy, China Medical University, No. 91, Hsueh Shih Road, Taichung City 404, Taiwan.
authors: Wen-Hsin
authors: WH
authors: Lin
authors: Chi-Rei
authors: CR
authors: Wu
authors: Tony J
authors: TJ
authors: Fang
authors: Jiun-Ting
authors: JT
authors: Guo
authors: Shi-Ying
authors: SY
authors: Huang
authors: Meng-Shiou
authors: MS
authors: Lee
authors: Hsin-Ling
authors: HL
authors: Yang
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology / Q000627:therapeutic use; D000898:Antibiosis; D001426:Bacterial Proteins / Q000378:metabolism; D002460:Cell Line; D043302:Cultured Milk Products / Q000382:microbiology*; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology; D005285:Fermentation; D005753:Gastric Mucosa / Q000166:cytology / Q000187:drug effects* / Q000382:microbiology; D016481:Helicobacter Infections / Q000188:drug therapy* / Q000378:metabolism / Q000382:microbiology; D016480:Helicobacter pylori / Q000187:drug effects*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007778:Lactobacillus / Q000145:classification; D019936:Probiotics / Q000494:pharmacology / Q000627:therapeutic use*; D013045:Species Specificity; D014510:Urease / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D014510:Urease