title: Fluorimetric study on the interaction between Norfloxacin and Proflavine hemisulphate.
abstract: The interaction between Norfloxacin (NF) and Proflavine hemisulphate (PF) was investigated by spectroscopic tools like UV-VIS absorption and Fluorescence spectroscopy. It was proved that fluorescence quenching of NF by PF is due to the formation of NF-PF complex which was supported by UV-VIS absorption study. The study of thermodynamic parameters suggested that the key interacting forces are hydrogen bond and van der Waal's interactions and the binding interaction was spontaneous. The distance r between NF and PF was obtained according to the Förster's theory of non-radiative energy transfer. The fluorescence quenching mechanism was applied to estimate PF directly from pharmaceutical samples.
authors: Department of Chemistry, Fluorescence Spectroscopy Research Laboratory, Shivaji University, Kolhapur 416 004 Maharashtra, India.
authors: Vishalkumar R
authors: VR
authors: More
authors: Prashant V
authors: PV
authors: Anbhule
authors: Sang H
authors: SH
authors: Lee
authors: Shivajirao R
authors: SR
authors: Patil
authors: Govind B
authors: GB
authors: Kolekar
mesh_terms: D005453:Fluorescence; D006860:Hydrogen Bonding; D057927:Hydrophobic and Hydrophilic Interactions; D009643:Norfloxacin / Q000737:chemistry*; D011370:Proflavine / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
chemical_list: D011370:Proflavine; D009643:Norfloxacin