title: Salivary bone turnover markers in healthy pre- and postmenopausal women: daily and seasonal rhythm.
abstract: No studies had investigated circadian and circannual rhythms of bone biomarkers in whole saliva. We evaluated the salivary daily and seasonal rhythm of carboxy-terminal telopeptide of type I collagen (CTX) and bone alkaline phosphatase (b-ALP). Forty clinical and oral healthy ambulatory pre- and postmenopausal women from two southern Argentine cities: Comodoro Rivadavia (latitude 45º S) and Ushuaia (latitude 54º S) were included in the study. CTX levels were evaluated in serum, urine, and saliva, and b-ALP levels were measured in serum and saliva. In both groups of women, salivary CTX showed a maximum percentage of change early in the morning (80%) and a minimum in the late afternoon (45%), similarly to the pattern observed in urinary samples. No daily rhythm was observed in serum or salivary b-ALP. 25-Hydroxyvitamin D levels decreased in winter vs. summer (p < 0.01) without differences between the two studied groups. Conversely, parathormone reached higher levels in winter (p < 0.05) which induced a slight non-significant increment in salivary CTX and b-ALP levels. The results showed that, as in serum and urinary samples, salivary CTX exhibits daily and a slight seasonal rhythmicity. Whole non-stimulated saliva is a useful tool to detect several oral and systemic diseases because it has important advantages compared to serum and urinary samples. Then, it may also be a promising sample to test changes in bone metabolism contributing to diagnose and to monitor the therapy of several metabolic bone diseases.
authors: Oral and General Biochemistry Department, School of Dentistry, Buenos Aires University, Buenos Aires, Argentina.
authors: Gretel G
authors: GG
authors: Pellegrini
authors: Macarena M S
authors: MM
authors: Gonzales Chaves
authors: Maria A
authors: MA
authors: Fajardo
authors: Graciela M
authors: GM
authors: Ponce
authors: Gloria I
authors: GI
authors: Toyos
authors: Fima
authors: F
authors: Lifshitz
authors: Silvia M
authors: SM
authors: Friedman
authors: Susana N
authors: SN
authors: Zeni
mesh_terms: D000328:Adult; D000469:Alkaline Phosphatase / Q000032:analysis* / Q000097:blood; D015415:Biomarkers / Q000032:analysis / Q000097:blood / Q000652:urine; D016723:Bone Remodeling / Q000502:physiology; D002118:Calcium / Q000097:blood; D002940:Circadian Rhythm; D024042:Collagen Type I / Q000032:analysis* / Q000097:blood / Q000652:urine; D005260:Female; D006801:Humans; D008875:Middle Aged; D010281:Parathyroid Hormone / Q000097:blood; D010455:Peptides / Q000032:analysis* / Q000097:blood / Q000652:urine; D010758:Phosphorus / Q000097:blood; D017698:Postmenopause / Q000097:blood / Q000378:metabolism* / Q000652:urine; D017697:Premenopause / Q000097:blood / Q000378:metabolism* / Q000652:urine; D012471:Salivary Proteins and Peptides / Q000032:analysis*; D012621:Seasons; D014807:Vitamin D / Q000031:analogs & derivatives / Q000097:blood
chemical_list: D015415:Biomarkers; D024042:Collagen Type I; D010281:Parathyroid Hormone; D010455:Peptides; D012471:Salivary Proteins and Peptides; C052929:collagen type I trimeric cross-linked peptide; D014807:Vitamin D; D010758:Phosphorus; C104450:25-hydroxyvitamin D; D000469:Alkaline Phosphatase; D002118:Calcium