title: Selection of antibodies against a single rare cell present in a heterogeneous population using phage display.
abstract: Here we describe a new method applying phage-displayed antibody libraries to the selection of antibodies against a single identified cell on a glass slide. This is the only described method that has successfully achieved selection of antibodies against a single rare cell in a heterogeneous population of cells. The phage library is incubated with the slide containing the identified rare cell of interest; incubation is followed by UV irradiation while protecting the target cell with a minute disc. The UV light inactivates all phages outside the shielded area by cross-linking the DNA constituting their genomes. The expected yield is between one and ten phage particles from a single cell selection. The encoded antibodies are subsequently produced monoclonally and tested for specificity. This method can be applied within a week to carry out ten or more individual cell selections. Including subsequent testing of antibody specificity, a specific antibody can be identified within 2 months.
authors: Department of Molecular Biology, Aarhus University, Aarhus, Denmark.
authors: Morten Dræby
authors: MD
authors: Sørensen
authors: Peter
authors: P
authors: Kristensen
mesh_terms: D000906:Antibodies / Q000032:analysis*; D000918:Antibody Specificity; D007158:Immunologic Techniques; D017404:In Situ Hybridization, Fluorescence; D008846:Micromanipulation; D019151:Peptide Library; D014466:Ultraviolet Rays
chemical_list: D000906:Antibodies; D019151:Peptide Library