title: Pseudotyping vesicular stomatitis virus with lymphocytic choriomeningitis virus glycoproteins enhances infectivity for glioma cells and minimizes neurotropism.
abstract: Vesicular stomatitis virus (VSV)-based oncolytic virotherapy has the potential to significantly improve the prognosis of aggressive malignancies such as brain cancer. However, VSV's inherent neurotoxicity has hindered clinical development so far. Given that this neurotropism is attributed to the glycoprotein VSV-G, VSV was pseudotyped with the nonneurotropic envelope glycoprotein of the lymphocytic choriomeningitis virus (LCMV-GP→VSV-GP). Compared to VSV, VSV-GP showed enhanced infectivity for brain cancer cells in vitro while sparing primary human and rat neurons in vitro and in vivo, respectively. In conclusion, VSV-GP has a much wider therapeutic window than VSV and is thus more suitable for clinical applications, especially in the brain.
authors: Division of Virology, Innsbruck Medical University, Innsbruck, Austria.
authors: Alexander
authors: A
authors: Muik
authors: Inna
authors: I
authors: Kneiske
authors: Marina
authors: M
authors: Werbizki
authors: Doris
authors: D
authors: Wilflingseder
authors: Tsanan
authors: T
authors: Giroglou
authors: Oliver
authors: O
authors: Ebert
authors: Anna
authors: A
authors: Kraft
authors: Ursula
authors: U
authors: Dietrich
authors: Gert
authors: G
authors: Zimmer
authors: Stefan
authors: S
authors: Momma
authors: Dorothee
authors: D
authors: von Laer
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004195:Disease Models, Animal; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D008217:Lymphocytic choriomeningitis virus / Q000235:genetics*; D009457:Neuroglia / Q000821:virology*; D009474:Neurons / Q000821:virology; D050504:Oncolytic Viruses / Q000235:genetics / Q000254:growth & development*; D051381:Rats; D018353:Rhabdoviridae Infections / Q000473:pathology / Q000821:virology; D018116:Vesiculovirus / Q000235:genetics / Q000254:growth & development*; D014764:Viral Proteins / Q000378:metabolism*; D056189:Viral Tropism
chemical_list: D006023:Glycoproteins; D014764:Viral Proteins