title: Histone deacetylase 3, not histone deacetylase 2, interacts with the major immediate early locus of human cytomegalovirus.
abstract: Evidence suggests that genome chromatinization and the posttranslational modification of histones are involved in the regulation of viral gene expression, including the human cytomegalovirus (HCMV). We performed a ChIP-on-Chip assay to determine whether histone deacetylases (HDACs) interact with HCMV genomic DNA on a global level. Surprisingly, we found that HDAC3, but not HDAC2, interacts not only with the major immediate early (MIE) promoter but also with the entire MIE locus, suggesting a heterogeneous interaction of HDAC3 with HCMV DNA. The interaction of HDAC3 with the MIE region is related to inhibition of viral replication because HDAC3 inhibitors enhanced HCMV replication.
authors: Department of Microbiology and Molecular Genetics, UMDNJ-New Jersey Medical School, 225 Warren Street, Newark, NJ 07101, USA.
authors: Ying
authors: Y
authors: Huang
authors: Qiyi
authors: Q
authors: Tang
authors: Michael
authors: M
authors: Nguyen
authors: Kalpana
authors: K
authors: Dulal
authors: Weijia
authors: W
authors: Wang
authors: Hua
authors: H
authors: Zhu
mesh_terms: D002460:Cell Line; D003587:Cytomegalovirus / Q000235:genetics* / Q000502:physiology; D003586:Cytomegalovirus Infections / Q000201:enzymology* / Q000821:virology; D015967:Gene Expression Regulation, Viral; D056464:Histone Deacetylase 2 / Q000235:genetics / Q000378:metabolism*; D006655:Histone Deacetylases / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D014779:Virus Replication; D000099186:Histone Deacetylase 3
chemical_list: D056464:Histone Deacetylase 2; D006655:Histone Deacetylases; D017874:Immediate-Early Proteins; D000099186:Histone Deacetylase 3