title: Quantification of α-methylene-λ-butyrolactone extracted from different parts ofAlstroemeria wilhelmina and evaluation of it's antigenicity using the guinea-pig maximization test.
abstract: To detect the type of contact dermatitis caused due to the handling ofAlstroemeria wilhelmina, 1% α-methylene-λ-butyrolactone (α-MBL) dissolved in physiological alien and a five-fold diluted saline solution of original extracts of flowers, leaves and stems of the flower were applied to guinea-pigs for extracts were applied to the animals as the challenge treatment in compliance with the guinea-pig maximization test (GMT). As a consequence, not only primary irritant dermatitis was observed, but also delayed type allergic contact dermatitis due toAlstroemeria wilhelmina was observed. α-MBL determined in the extracts using high-performance liquid chromatography (HPLC) was found to be the biochemical material cause of the contact dermatitis. the flower region contained α-MBL in the highest concentrations compared with those of the leaves and stems. Therefore, the quantification of α-MBL in the extracts was concluded as being a useful evaluating method for contact dermatitis due to the handling ofAlstroemeria.
authors: Department of Hygiene, Kumamoto University School of Medicine, Honjo 2-2-1, 860-0811, Kumamoto, Japan, haradako@gpo.kumamoto.u.ac.jp.
authors: Koichi
authors: K
authors: Harada
authors: Shoko
authors: S
authors: Ohmori
authors: Chang-Nian
authors: CN
authors: Wei
authors: Yoshiki
authors: Y
authors: Arimatsu
authors: Atsushi
authors: A
authors: Ueda