title: Lepidopteran transcriptome analysis following infection by phylogenetically unrelated polydnaviruses highlights differential and common responses.
abstract: The Polydnaviridae is a family of double-stranded DNA viruses that are symbionts of parasitoid wasps. The family is currently divided into two genera, the Ichnovirus (IV) and Bracovirus (BV), which are associated with wasps in the families Ichneumonidae and Braconidae, respectively. IVs and BVs have similar immunosuppressive and developmental effects on parasitized hosts but their encapsidated genomes largely encode different genes. To assess whether IV and BV infection has similar or disparate effects on the transcriptome of shared hosts, we characterized the effects of Hyposoter didymator Ichnovirus (HdIV) and Microplitis demolitor Bracovirus (MdBV) on the fat body and hemocyte transcriptome of Spodoptera frugiperda (Lepidoptera: Noctuidae). Our results indicated that HdIV and MdBV infection alters the abundance of a relatively low proportion of S. frugiperda transcripts at 24 h post-infection. A majority of the transcripts affected by infection also differed between MdBV and HdIV. However, we did identify some host transcripts that were similarly affected by both viruses. A majority of these genes were transcribed in the fat body and most belonged to functional classes with roles in immunity, detoxification, or cell structure. Particularly prominent in this suite of transcripts were genes encoding for predicted motor-related and collagen IV-like proteins. Overall, our data suggest that the broadly similar effects that HdIV and MdBV have on host growth and immunity are not due to these viruses inducing profound changes in host gene expression. Given though that IVs and BVs encode few shared genes, the host transcripts that are similarly affected by HdIV and MdBV could indicate convergence by each virus to target a few processes at the level of transcription that are important for successful parasitism of hosts by H. didymator and M. demolitor.
authors: UMR1333, INRA, Université Montpellier 2, Place Eugène Bataillon, cc101, F-34095 Montpellier Cedex 5, France.
authors: Bertille
authors: B
authors: Provost
authors: Véronique
authors: V
authors: Jouan
authors: Frédérique
authors: F
authors: Hilliou
authors: Pierre
authors: P
authors: Delobel
authors: Pauline
authors: P
authors: Bernardo
authors: Marc
authors: M
authors: Ravallec
authors: François
authors: F
authors: Cousserans
authors: Eric
authors: E
authors: Wajnberg
authors: Isabelle
authors: I
authors: Darboux
authors: Philippe
authors: P
authors: Fournier
authors: Michael R
authors: MR
authors: Strand
authors: Anne-Nathalie
authors: AN
authors: Volkoff
mesh_terms: D000818:Animals; D003094:Collagen / Q000235:genetics / Q000378:metabolism; D005216:Fat Body / Q000378:metabolism*; D015870:Gene Expression / Q000276:immunology*; D020869:Gene Expression Profiling; D016679:Genome, Viral; D006434:Hemocytes / Q000378:metabolism*; D019476:Insect Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D018071:Polydnaviridae / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018411:Spodoptera / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D013559:Symbiosis / Q000235:genetics / Q000276:immunology; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics; D014863:Wasps / Q000235:genetics / Q000276:immunology / Q000378:metabolism
chemical_list: D019476:Insect Proteins; D012333:RNA, Messenger; D014764:Viral Proteins; D003094:Collagen