title: Evaluation of the effectiveness of an ointment based on Alkannins/Shikonins on second intention wound healing in the dog.
abstract: The enantiomeric naphthoquinones alkannins and shikonins (A/S) have been established as potent wound healing agents. The purpose of this study was to evaluate the effectiveness of an A/S based ointment for humans on second intention wound healing in the dog, as compared to wound flushing with Lactated Ringer's solution (LRS). Ten mixed breed dogs, aged 2 to 5 y, were used. One 2.5 × 2.5 cm full-thickness skin defect was created on the lateral aspect of each arm for subjective evaluation, laser-Doppler flowmetry (LDF), and planimetry. Additionally, 3 matching 2 × 2 cm wounds were created on opposite sides of the dorsal midline for histologic evaluation. Wounds were treated once daily with the A/S based ointment on the right side and by flushing with LRS on the left until healed (about 20 d). During the healing process, tissue perfusion (mean LDF value) was found to be significantly higher on the side treated with the A/S based ointment compared with the LRS-treated side. Histologically, angiogenesis (on days 4 and 11), collagen production score (on days 4, 11, and 20), and epithelial thickness score (on day 11) were significantly higher in the wounds treated with the A/S based ointment. Wound size, as evaluated by planimetry, decreased significantly from day 0 to day 20 on both sides, but no significant differences were found between the A/S based ointment and LRS-treated wounds.
authors: Department of Clinical Studies, Companion Animal Clinic, Faculty of Veterinary Medicine, Aristotle University of Thessaloniki, Greece. marikara@vet.auth.gr
authors: Maria
authors: M
authors: Karayannopoulou
authors: Vassiliki
authors: V
authors: Tsioli
authors: Panayiotis
authors: P
authors: Loukopoulos
authors: Tilemahos L
authors: TL
authors: Anagnostou
authors: Nikolaos
authors: N
authors: Giannakas
authors: Ioannis
authors: I
authors: Savvas
authors: Lysimachos G
authors: LG
authors: Papazoglou
authors: Eleni
authors: E
authors: Kaldrymidou
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use*; D004285:Dogs; D004334:Drug Administration Schedule / Q000662:veterinary; D004338:Drug Combinations; D005260:Female; D005552:Forelimb / Q000293:injuries; D007552:Isotonic Solutions / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D017078:Laser-Doppler Flowmetry; D008297:Male; D009285:Naphthoquinones / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use*; D000077325:Ringer's Lactate; D012867:Skin / Q000293:injuries; D017695:Soft Tissue Injuries / Q000473:pathology / Q000628:therapy / Q000662:veterinary*; D007507:Therapeutic Irrigation / Q000662:veterinary; D016896:Treatment Outcome; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D004338:Drug Combinations; D007552:Isotonic Solutions; D009285:Naphthoquinones; D000077325:Ringer's Lactate; C018204:alkannin; C016101:shikonin