title: Correlation between Heterogeneous Expression of Sialyltransferases and MUC16 in Ovarian Tumor Tissues.
abstract: BACKGROUND
  A number of glycoproteins such as CA125 are abnormally glycosylated in ovarian cancers. Most aberrant glycosylations are a result of altered sialyltransferase (ST) expression. The aim of this study was to evaluate the expression of 6 STs and MUC16, and their correlations in benign and malignant ovarian tissues.
  
  
  METHODS
  mRNA expression of 6 STs and MUC16 was assessed in 16 human ovarian tumors (7 benign, 9 malignant) by real-time quantitative polymerase chain reaction (RTQ-PCR).
  
  
  RESULTS
  mRNA of ST6GAL I and ST3GAL I was not significantly upregulated in ovarian cancer tissues, while ST6GAL II and ST3GAL IV were not significantly increased in benign tumors. There was no change between ST3GAL III and ST3GAL VI expression and tumor subtypes. MUC16 was significantly increased in carcinoma tissue. Significant correlation was found between ST3GAL III and ST3GAL IV. MUC16 correlated with ST3GAL VI and ST6GAL I. ST6GAL I correlated well with ST3GAL VI. ST6GAL II correlated significantly with ST3GAL III and ST3GAL IV.
  
  
  CONCLUSIONS
  The given STs and MUC16 can be expressed at a heterogeneous level as a consequence of oncogenic transformation of the ovary. A strong correlation between MUC16 and STs may impact specifically on the glycosylation of MUC16.
authors: Laboratory of Biochemistry and Molecular Biology, Faculty of Pharmacy, Monastir, Tunisia. bouanenehouda2@yahoo.fr
authors: Houda
authors: H
authors: Bouanene
authors: Wassila
authors: W
authors: Sahrawi
authors: Moncef
authors: M
authors: Mokni
authors: Leila Ben
authors: LB
authors: Fatma
authors: Amira
authors: A
authors: Bouriga
authors: Halima Ben
authors: HB
authors: Limen
authors: Hédi
authors: H
authors: Khairi
authors: Slim Ben
authors: SB
authors: Ahmed
authors: Abdelhédi
authors: A
authors: Miled
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism*; D018394:CA-125 Antigen / Q000378:metabolism*; D005260:Female; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000378:metabolism*; D010051:Ovarian Neoplasms / Q000378:metabolism*; D012799:Sialyltransferases / Q000378:metabolism*; D013223:Statistics as Topic
chemical_list: D014408:Biomarkers, Tumor; D018394:CA-125 Antigen; C451759:MUC16 protein, human; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D012799:Sialyltransferases