title: RNA interference mediated knockdown of apolipophorin-III leads to knockdown of manganese superoxide dismutase in Hyphantria cunea.
abstract: Apolipophorin-III (apoLp-III), a hemolymph protein that facilitates lipid transport in aqueous media in insects was recently shown to play a role in insect immune activation. Here, we report another novel possible function of apoLp-III in insects. To identify genes affected by apoLp-III expression in larvae, we decreased endogenous apoLp-III mRNA in Hyphantria cunea (Hc) through RNA interference; subsequently, we observed lower levels of antioxidant enzymes, including manganese superoxide dismutase (MnSOD), glutathione S-transferase, and immune proteins. Knockdown of Hc apoLp-III led to decreased MnSOD expression in fat body tissues and elevated superoxide anion levels in Hc fat body cells, suggesting that Hc apoLp-III is involved in the action and/or expression of antioxidant enzymes, especially MnSOD.
authors: Division of Applied Life Science, Gyeongsang National University, Jinju, Republic of Korea.
authors: Yong Il
authors: YI
authors: Kim
authors: Hong Ja
authors: HJ
authors: Kim
authors: Yong Min
authors: YM
authors: Kwon
authors: Young Jin
authors: YJ
authors: Kang
authors: In Hee
authors: IH
authors: Lee
authors: Byung Rae
authors: BR
authors: Jin
authors: Yeon Soo
authors: YS
authors: Han
authors: Iksoo
authors: I
authors: Kim
authors: Hyang Mi
authors: HM
authors: Cheon
authors: Nam Gyu
authors: NG
authors: Ha
authors: Sook Jae
authors: SJ
authors: Seo
mesh_terms: D000818:Animals; D001053:Apolipoproteins / Q000235:genetics*; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D055785:Gene Knockdown Techniques; D009036:Moths / Q000201:enzymology*; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism*
chemical_list: D001053:Apolipoproteins; D017931:DNA Primers; D012333:RNA, Messenger; C042470:apolipophorin III; D013482:Superoxide Dismutase