title: Influencing factors for cure of clonorchiasis by praziquantel therapy: infection burden and CYP3A5 gene polymorphism.
abstract: Chemotherapy of clonorchiasis with praziquantel (PZQ) is effective but about 15% of treated cases have been reported uncured. The present study investigated correlation of single nucleotide polymorphisms (SNPs) of the cytochrome P450 gene, CYP3A5 and cure of clonorchiasis. A total of 346 egg passing residents were subjected and treated by 3 doses of 25 mg/kg PZQ. Reexamination recognized 33 (9.5%) uncured and 313 cured. Numbers of eggs per gram of feces (EPGs) before treatment were significantly lower in the cured group than in the uncured group (2,011.2±3,600.0 vs 4,998.5±7,012.0, P<0.001). DNAs of the subjects were screened for SNPs at 7 locations of CYP3A5 using PCR. In the uncured group, the SNP frequencies at g.-20555G>A and g.27526C>T of CYP3A5 were 15.2% and 9.1% while those were 3.8% and 1.0%, respectively, in the cured group. The cure rate was significantly lower in the cases with SNP at g.27526C>T and EPGs≥1,000. In conclusion, EPGs and SNPs of CYP3A5 are factors which influence cure of clonorchiasis by PZQ therapy. It is strongly suggested to recommend 2-day medication for individuals with high EPGs≥1,000.
authors: Department of Parasitology and Tropical Medicine, and Institute of Infectious Diseases, Seoul National University College of Medicine, Seoul 110-799, Korea.
authors: Chung Hyeon
authors: CH
authors: Kim
authors: Jeong-Keun
authors: JK
authors: Lee
authors: Byung-Suk
authors: BS
authors: Chung
authors: Shunyu
authors: S
authors: Li
authors: Min-Ho
authors: MH
authors: Choi
authors: Sung-Tae
authors: ST
authors: Hong
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D002648:Child; D003003:Clonorchiasis / Q000188:drug therapy* / Q000235:genetics* / Q000469:parasitology; D003004:Clonorchis sinensis / Q000302:isolation & purification / Q000502:physiology; D051544:Cytochrome P-450 CYP3A / Q000235:genetics*; D005243:Feces / Q000469:parasitology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011223:Praziquantel / Q000627:therapeutic use*; D016896:Treatment Outcome; D055815:Young Adult
chemical_list: D011223:Praziquantel; C510164:CYP3A5 protein, human; D051544:Cytochrome P-450 CYP3A