title: DNA damage in Mexican women with cervical dysplasia evaluated by comet assay.
abstract: OBJECTIVE
  To investigate the association between the progressive stage of cervical dysplasia and DNA damage by comet assay.
  
  
  METHODS
  A hospital-based, unmatched, case-control study was performed. DNA damage levels (none, low, medium and high) in the cervical epithelial cells of 31 women (10 with low grade squamous intraepithelial lesions [LSIL], 10 with high grade [HSIL] and 11 with no cervical lesion) were evaluated using the comet assay.
  
  
  RESULTS
  A significant increase in medium DNA damage was observed in women with HSIL (17 +/- 8.9) relative to that in the control women (9 +/- 6.1). A significant increase in high DNA damage was also observed in women with LSIL (23 +/- 15.4) or HSIL (32 +/- 13.1) relative to that in the control women (12 +/- 7.9).
  
  
  CONCLUSIONS
  These findings confirm that the grade of a cervical lesion correlates with the degree of genomic instability.
authors: Division of Genetics, Centro de Investigación Biomédica del Noreste, Instituto Mexicano del Seguro Social, Universidad Autónoma de Nuevo León, Monterrey, Mexico. elvacortes@cibinmty.net
authors: Elva I
authors: EI
authors: Cortés-Gutiérrez
authors: Martha I
authors: MI
authors: Dávila-Rodríguez
authors: Estela A
authors: EA
authors: Zamudio-González
authors: Miguel E
authors: ME
authors: Aguado-Barrera
authors: Javier
authors: J
authors: Vargas-Villarreal
authors: Ricardo M
authors: RM
authors: Cerda-Flores
mesh_terms: D000328:Adult; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology; D016022:Case-Control Studies; D020552:Comet Assay / Q000379:methods*; D004249:DNA Damage; D004847:Epithelial Cells / Q000473:pathology* / Q000502:physiology; D005260:Female; D042822:Genomic Instability; D006801:Humans; D008800:Mexico; D008875:Middle Aged; D009367:Neoplasm Staging / Q000379:methods; D002578:Uterine Cervical Dysplasia / Q000235:genetics* / Q000473:pathology*; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000473:pathology; D055815:Young Adult