title: The cytotoxicity of microglass fibers on alveolar macrophages of fischer 344 rats evaluated by cell magnetometry, cytochemisry and morphology.
abstract: OBJECTIVE
  The toxicity of microglass fibers (MG), one of the man-made mineral fibers, has not been sufficiently evaluated. The aim of the current study was to evaluate the cytotoxicity of MGin vitro.
  
  
  METHODS
  Alveolar macrophages were obtained from the bronchoalveolar lavage of male F344/N rats. The macrophages were exposed to MG at concentrations of 0, 40, 80, 160 and 320 μg/ml. The effects of MG on the macrophages were examined by cell magnetometry, LDH assay and morphological observation.
  
  
  RESULTS
  In the cell magnetometry experiment, a significant delay of relaxation (the reduction of remanent magnetic field strength) was observed in the cells treated with 160 and 320 μg/ml of MG in a dose-dependent manner. A significant increase in LDH release was also observed in the cells with 160 and 320 μg/ml in a dose-dependent manner. Changes in the cytoskeleton were observed after exposure to MG by immunofluorescent microscopy using an α-tubulin antibody.
  
  
  CONCLUSIONS
  The cytotoxicity of MG on alveolar macrophages was demonstrated with cell magnetometry. The mechanism of the toxic effects of MG was related to cytoskeleton damage.
authors: Department of Preventive Medicine and Public Health, Kitasato University School of Medicine, 1-15-1 Kitasato, 228-8555, Sagamihara, Kanagawa, Japan, hisako@med.kitasato-a.ac.jp.
authors: Hisako
authors: H
authors: Shinji
authors: Mitsuyasu
authors: M
authors: Watanabe
authors: Yuichiro
authors: Y
authors: Kudo
authors: Masato
authors: M
authors: Niitsuya
authors: Masashi
authors: M
authors: Tsunoda
authors: Toshihiko
authors: T
authors: Satoh
authors: Yasuhiro
authors: Y
authors: Sakai
authors: Makoto
authors: M
authors: Kotani
authors: Yoshiharu
authors: Y
authors: Aizawa