title: Determination of dioxin concentrations in fish and seafood samples using a highly sensitive reporter cell line, DR-EcoScreen cells.
abstract: There is a strong need for the development of relatively rapid and low-cost bioassays for the determination of polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs), and dioxin-like polychlorinated biphenyls (dl-PCBs) in environmental and food samples. In this study, we applied a reporter gene assay using DR-EcoScreen cells (DR-cell assay), which is highly sensitive to dioxins, to the determination of PCDD/Fs and dl-PCBs in fish and seafood samples. The PCDD/Fs and dl-PCBs were extracted from homogenated samples (10 g) of 30 fish and shellfish, purified by clean-up procedure using a multilayered silica gel column and an alumina column, and applied to DR-cell assay. Interestingly, the bioanalytical equivalent (BEQ) values obtained from the DR-cell assay [<0.1∼5.4 pg BEQ g(-1) wet weight (ww)] were closely correlated with the toxicity equivalent (TEQ) values from conventional high-resolution gas chromatography/high-resolution mass spectrometry (HRGC-HRMS) analysis (r(2)=0.912), and the slope of regression line was 0.913. Therefore, we multiplied the BEQ values from the DR-cell assay by a conversion coefficient (1.095, the reciprocal of 0.913) to approximate the TEQ values from the HRGC-HRMS analysis. Furthermore, we used this DR-cell assay to perform a prescreening test of PCDD/Fs and dl-PCBs in 16 fish and seafood samples purchased from a supermarket, revealing that a sample from the fatty flesh of a bluefin tuna exceeded 8 pg TEQ g(-1)ww (the European Union-tolerance limit). Taken together, these results suggest that the DR-cell assay might be applicable as a rapid and low-cost prescreening method to determine dioxin levels in fish and seafood samples.
authors: Hokkaido Institute of Public Health, Kita-19, Nishi-12, Kita-ku, Sapporo 060-0819, Japan. kojima@iph.pref.hokkaido.jp
authors: Hiroyuki
authors: H
authors: Kojima
authors: Shinji
authors: S
authors: Takeuchi
authors: Tomoaki
authors: T
authors: Tsutsumi
authors: Katsuyuki
authors: K
authors: Yamaguchi
authors: Katsunori
authors: K
authors: Anezaki
authors: Keiko
authors: K
authors: Kubo
authors: Mitsuru
authors: M
authors: Iida
authors: Tetsuo
authors: T
authors: Takahashi
authors: Satoshi
authors: S
authors: Kobayashi
authors: Kazuo
authors: K
authors: Jin
authors: Tadanori
authors: T
authors: Nagai
mesh_terms: D000818:Animals; D001572:Benzofurans / Q000032:analysis / Q000378:metabolism; D001681:Biological Assay; D002460:Cell Line; D004147:Dioxins / Q000032:analysis / Q000378:metabolism*; D004784:Environmental Monitoring / Q000379:methods*; D005399:Fishes / Q000378:metabolism*; D005506:Food Contamination / Q000032:analysis / Q000706:statistics & numerical data; D017930:Genes, Reporter; D008099:Liver / Q000378:metabolism; D051379:Mice; D011078:Polychlorinated Biphenyls / Q000032:analysis / Q000378:metabolism; D000072317:Polychlorinated Dibenzodioxins / Q000031:analogs & derivatives / Q000032:analysis / Q000378:metabolism; D011108:Polymers / Q000032:analysis / Q000378:metabolism; D017747:Seafood / Q000032:analysis* / Q000706:statistics & numerical data; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000378:metabolism*; D014877:Water Pollution, Chemical / Q000706:statistics & numerical data
chemical_list: D001572:Benzofurans; D004147:Dioxins; D000072317:Polychlorinated Dibenzodioxins; D011108:Polymers; D014874:Water Pollutants, Chemical; C016068:polychlorodibenzofuran; D011078:Polychlorinated Biphenyls