title: Heterogeneity of macrophage populations and expression of galectin-3 in cutaneous wound healing in rats.
abstract: The aim of this study was to investigate the properties of macrophages that infiltrated the sites of cutaneous wound healing in rats between 1 and 26 days post wounding (dpw). During the inflammation phase (1-3 dpw), ED1(+) (CD68(+)) macrophages with enhanced lysosomal activity dominated. From 5 to 7 dpw there was formation of granulation tissue as indicated by the presence of myofibroblasts expressing α-smooth muscle actin. At this stage, ED2(+) (CD163(+)) macrophages, capable of producing inflammatory factors, were dominant. The majority of ED1(+) macrophages expressed galectin-3, a regulator of fibrosis. Corresponding to the increased numbers of ED1(+) and ED2(+) macrophages at 3-9 dpw, there was increased expression of genes encoding transforming growth factor-β1 (a major fibrogenic factor), monocyte chemoattractant protein-1 and colony stimulating factor-1. These macrophage-related factors might contribute to inflammation and formation of granulation tissue. OX6(+) macrophages expressing class II molecules of the major histocompatibility complex became predominant in the healing stages (15-26 dpw), indicating important roles for antigen-presenting cells in tissue remodelling. The OX6(+) macrophages were most likely derived from ED1(+) macrophages. The results of this study show that infiltration of phenotypically- and functionally-distinct macrophage populations characterizes different stages of the wound healing process.
authors: Division of Veterinary Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka, Japan.
authors: V
authors: V
authors: Juniantito
authors: T
authors: T
authors: Izawa
authors: E
authors: E
authors: Yamamoto
authors: F
authors: F
authors: Murai
authors: M
authors: M
authors: Kuwamura
authors: J
authors: J
authors: Yamate
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis; D002452:Cell Count; D019070:Cell Lineage; D005355:Fibrosis; D037502:Galectin 3 / Q000096:biosynthesis* / Q000235:genetics; D006097:Granulation Tissue / Q000378:metabolism; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D008264:Macrophages / Q000145:classification / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D011956:Receptors, Cell Surface / Q000032:analysis; D012867:Skin / Q000293:injuries* / Q000378:metabolism / Q000473:pathology; D014945:Wound Healing / Q000502:physiology*; D000098902:CD163 Antigen; D000097382:CD68 Molecule
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D037502:Galectin 3; D000949:Histocompatibility Antigens Class II; D011956:Receptors, Cell Surface; D000098902:CD163 Antigen; C539355:CD68 protein, rat; D000097382:CD68 Molecule