title: Different transcriptional profiles of human monocyte-derived dendritic cells infected with distinct strains of Mycobacterium tuberculosis and Mycobacterium bovis bacillus Calmette-Guérin.
abstract: In order to analyze dendritic cells (DCs) activation following infection with different mycobacterial strains, we studied the expression profiles of 165 genes of human monocyte-derived DCs infected with H37Rv, a virulent Mycobacterium tuberculosis (MTB) laboratory strain, CMT97, a clinical MTB isolate, Mycobacterium bovis bacillus Calmette-Guérin (BCG), Aventis Pasteur, and BCG Japan, both employed as vaccine against tuberculosis. The analysis of the gene expression reveals that, despite a set of genes similarly modulated, DCs response resulted strain dependent. In particular, H37Rv significantly upregulated EBI3 expression compared with BCG Japan, while it was the only strain that failed to release a significant IL-10 amount. Of note, BCG Japan showed a marked increase in CCR7 and TNF-α expression regarding both MTB strains and it resulted the only strain failing in exponential intracellular growth. Our results suggest that DCs display the ability to elicit a tailored strain-specific immune response.
authors: Institute of Cell Biology and Neurobiology, National Research Council, Rome, Italy. nunzia.sanarico@iss.it
authors: Nunzia
authors: N
authors: Sanarico
authors: Alessia
authors: A
authors: Colone
authors: Manuela
authors: M
authors: Grassi
authors: Viviana
authors: V
authors: Speranza
authors: Daniela
authors: D
authors: Giovannini
authors: Antonio
authors: A
authors: Ciaramella
authors: Vittorio
authors: V
authors: Colizzi
authors: Francesca
authors: F
authors: Mariani
mesh_terms: D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D020869:Gene Expression Profiling; D006801:Humans; D016130:Immunophenotyping; D016753:Interleukin-10 / Q000235:genetics / Q000378:metabolism; D007378:Interleukins / Q000235:genetics / Q000378:metabolism; D015778:Minor Histocompatibility Antigens; D009000:Monocytes / Q000473:pathology; D009163:Mycobacterium bovis / Q000276:immunology* / Q000472:pathogenicity; D009169:Mycobacterium tuberculosis / Q000276:immunology* / Q000472:pathogenicity; D054400:Receptors, CCR7 / Q000235:genetics / Q000378:metabolism; D013045:Species Specificity; D014376:Tuberculosis / Q000276:immunology* / Q000382:microbiology / Q000517:prevention & control; D032581:Tuberculosis Vaccines; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism; D014774:Virulence
chemical_list: C515370:CCR7 protein, human; C097732:EBI3 protein, human; D007378:Interleukins; D015778:Minor Histocompatibility Antigens; D054400:Receptors, CCR7; D032581:Tuberculosis Vaccines; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10