title: Protective effect of intestinal trefoil factor on injury of intestinal epithelial tight junction induced by platelet activating factor.
abstract: Intestinal barrier dysfunction plays an important role in the pathogenesis of inflammatory bowel disease (IBD). To evaluate the effect of intestinal trefoil factor (ITF) on increased intestinal permeability and its association with tight junction proteins, an in vitro intestinal epithelia barrier model was established with Caco-2 cells and treated with platelet-activating factor (PAF). We found that exposing cells to 0.3 M ITF (30 min before or 30 min after PAF treatment) attenuated the PAF-induced changes in transepithelial electrical resistance and Lucifer yellow flux. A quantitative RT-PCR and western blot analysis revealed that ITF suppressed PAF-induced downregulation of tight junction proteins claudin-1 and ZO-1 expression; furthermore, an abnormal localization and distribution of these proteins was inhibited, as assessed by immunofluorescence staining. These results suggest that ITF decreases mucosal permeability and shows potential as a therapy for treating IBD.
authors: Department of Pediatric, Affiliated Shengjing Hospital, China Medical University, Shenyang, 110004, Liaoning, China.
authors: Ling-fen
authors: LF
authors: Xu
authors: Xu
authors: X
authors: Teng
authors: Jing
authors: J
authors: Guo
authors: Mei
authors: M
authors: Sun
mesh_terms: D001692:Biological Transport / Q000187:drug effects; D018938:Caco-2 Cells; D045744:Cell Line, Tumor; D062445:Claudin-1; D017097:Electric Impedance; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000188:drug therapy / Q000473:pathology*; D007413:Intestinal Mucosa / Q000187:drug effects / Q000473:pathology / Q000502:physiology / Q000648:ultrastructure*; D008564:Membrane Potentials / Q000187:drug effects*; D008565:Membrane Proteins / Q000378:metabolism; D010455:Peptides / Q000378:metabolism / Q000494:pharmacology*; D010750:Phosphoproteins / Q000378:metabolism; D010972:Platelet Activating Factor / Q000494:pharmacology; D019108:Tight Junctions / Q000187:drug effects* / Q000648:ultrastructure*; D000071164:Trefoil Factor-2; D062826:Zonula Occludens-1 Protein
chemical_list: C568108:CLDN1 protein, human; D062445:Claudin-1; D008565:Membrane Proteins; D010455:Peptides; D010750:Phosphoproteins; D010972:Platelet Activating Factor; C569042:TJP1 protein, human; D000071164:Trefoil Factor-2; D062826:Zonula Occludens-1 Protein