title: Establishment of a stable CHO cell line with high level expression of recombinant porcine IFN-β.
abstract: A CHO cell clone (CHO-PoIFN-β) with stable porcine IFN-β expression under control of CMV promoter was selected under G418 pressure. In a 25cm(2) cell culture flask (5 ml culture medium), the cumulative protein yield of recombinant PoIFN-β reached 2.3×10(6) IU/ml. This cells clone maintained stable expression for at least 20 generations even in the absence of G418 selection pressure. The expressed recombinant PoIFN-β could induce the expression of porcine Mx protein in PK15 cells, and activate the chicken Mx promoter-controlled luciferase reporter gene expression, confirming that the recombinant PoIFN-β has the biological activity of natural porcine type-I interferon. In addition, the recombinant PoIFN-β fully protected PK15 cells against 1000 TCID(50) of porcine transmissible gastroenteritis virus and pseudo-rabies virus infection, demonstrating its high potential in therapeutic applications. This is the first report of establishing a mammalian cell line with stable expression of porcine IFN-β.
authors: National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), 427 Maduan Street, Harbin 150001, People's Republic of China.
authors: Weiye
authors: W
authors: Chen
authors: Wenyan
authors: W
authors: Cao
authors: Huijun
authors: H
authors: Zhao
authors: Qianqian
authors: Q
authors: Hu
authors: Linmao
authors: L
authors: Qu
authors: Sen
authors: S
authors: Hu
authors: Jinying
authors: J
authors: Ge
authors: Zhiyuan
authors: Z
authors: Wen
authors: Xijun
authors: X
authors: Wang
authors: Haobo
authors: H
authors: Li
authors: Kehe
authors: K
authors: Huang
authors: Zhigao
authors: Z
authors: Bu
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry; D000998:Antiviral Agents / Q000494:pharmacology; D016466:CHO Cells; D002645:Chickens; D006224:Cricetinae; D003412:Cricetulus; D004285:Dogs; D016899:Interferon-beta / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism; D010957:Plasmids / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000378:metabolism; D013552:Swine; D013997:Time Factors
chemical_list: D000906:Antibodies; D000998:Antiviral Agents; D011994:Recombinant Proteins; D016899:Interferon-beta