title: One-step separation and purification of hydrolysable tannins from Geranium wilfordii Maxim by adsorption chromatography on cross-linked 12% agarose gel.
abstract: The hydrolysable tannins corilagin and geraniin, the major active components of the traditional Chinese medicine Geranium wilfordii Maxim, have been separated and purified from crude extracts in one step by adsorption chromatography on cross-linked 12% agarose gel (Superose 12 10/300 GL). The separation was achieved by gradient elution using mobile phase A composed of 5% ethanol and 5% acetic acid and mobile phase B composed of 30% ethanol and 30% acetic acid. The gradients were composed as follows: 0-240 mL, 0-25% B; 240-480 mL, 25-40% B; after 480 mL, 100% B. The purities of the collected corilagin and geraniin were 92.4 and 87.2%, and the corresponding yields were 88.0 and 76.8%, respectively.
authors: School of Environmental and Biological Science and Technology, Dalian University of Technology, Dalian, P R China.
authors: Dan
authors: D
authors: Liu
authors: Yan
authors: Y
authors: Ma
authors: Ye
authors: Y
authors: Wang
authors: Zhiguo
authors: Z
authors: Su
authors: Ming
authors: M
authors: Gu
authors: Jan-Christer
authors: JC
authors: Janson
mesh_terms: D002845:Chromatography / Q000295:instrumentation / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification*; D031315:Geranium / Q000737:chemistry*; D047348:Hydrolyzable Tannins / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D004365:Drugs, Chinese Herbal; D047348:Hydrolyzable Tannins