title: Molecular impact and inducible factors associated with adenovirus infection in hematopoietic stem [corrected] cell transplant patients.
abstract: OBJECTIVE
  Latent and active adenovirus infections are detected in 5% to 20% of hematopoietic stem cell transplant (HSCT) patients. In addition to the significant role of adenoviral infection in the pathogenesis of late-onset hemorrhagic cystitis in HSCT patients, adenovirus infections may have possible roles in undefined posttransplant clinical complications. Therefore, pre- and posttransplantation we studied the prevalence and role of adenoviral infections among HSCT clinical syndromes using molecular methods.
  
  
  METHODS
  In this cross-sectional study between 2005 and 2008. We collected 470 EDTA-treated blood samples from 125 HSCT recipients, including 70 (56%) men and 55 (44%) women. The 52 (41.6%) HSCT patients underwent autologous grafts and the other 73 (58.4%), from related donors. One EDTA-treated blood sample was collected from all recipients pretransplantation. Also once per week for 3 months we were collected blood samples from HSCT patients to evaluate the prevalence of adenovirus DNA infection by a qualitative in house polymerase chain reaction method.
  
  
  RESULTS
  The adenovirus genome was diagnosed in 2/75 (2.7%) HSCT patient samples pretransplantation. There were 28/395 (7.1%) plasma samples of transplant patients infected with adenovirus DNA. Graft-versus-host disease (GVHD) clinical complications were observed in six adenovirus-infected transplant recipients; there was a significant correlation between these viral infections and GVHD clinical presentation.
  
  
  CONCLUSIONS
  The high prevalence of adenovirus infection in HSCT recipients pre- and posttransplantation, was significantly related to GVHD symptoms, enforcing the important pathogenic role of these viral infections in clinical complications post-HSCT.
authors: Shiraz Transplant Research Center, Shiraz University of Medical Sciences, Nemazee Hospital, Shiraz, Iran.
authors: R
authors: R
authors: Yaghobi
authors: M J
authors: MJ
authors: Langari
authors: M
authors: M
authors: Ramzi
authors: B
authors: B
authors: Geramizadeh
authors: M
authors: M
authors: Zakerinia
authors: H N
authors: HN
authors: Khojasteh
authors: M
authors: M
authors: Dehghani
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000257:Adenoviridae Infections / Q000175:diagnosis; D000293:Adolescent; D000328:Adult; D002648:Child; D003430:Cross-Sectional Studies; D004492:Edetic Acid / Q000737:chemistry; D005260:Female; D016679:Genome, Viral; D006086:Graft vs Host Disease; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D012867:Skin / Q000821:virology; D016038:Skin Transplantation / Q000379:methods*
chemical_list: D004492:Edetic Acid