title: Prominent expression of sialyl Lewis X-capped core 2-branched O-glycans on high endothelial venule-like vessels in gastric MALT lymphoma.
abstract: High endothelial venule (HEV)-like vessels have been observed in gastric B cell lymphoma of mucosa-associated lymphoid tissue type (MALT lymphoma), as well as in its preceding lesion, chronic Helicobacter pylori gastritis. Previously we reported that glycans on HEV-like vessels in the latter lesion served as L-selectin ligands, although their function is unclear. We have investigated sialyl Lewis X (sLeX)-related glycoepitopes and found that MECA-79(-) /HECA-452(+) /NCC-ST-439(+) HEV-like vessels preferentially mark gastric MALT lymphoma compared to chronic H. pylori gastritis. We then constructed CHO cell lines expressing potential MECA-79(-) /HECA-452(+) /NCC-ST-439(+) glycans, as well as other sLeX-type glycans, on CD34 and evaluated L-selectin binding to those cells, using L-selectin-IgM chimera binding and lymphocyte adhesion assays. L-selectin-IgM chimeras bound to CHO cells expressing 6-sulpho-sLeX attached to core 2-branched O-glycans with or without 6-sulpho-sLeX attached to extended core 1 O-glycans, but only marginally to other CHO cell lines. By contrast, CHO cells expressing 6-sulpho-sLeX attached to extended core 1 and/or core 2-branched O-glycans, as well as non-sulphated sLeX attached to core 2-branched O-glycans, showed substantial lymphocyte binding, while binding was negligible on lines expressing 6-sulpho- and non-sulphated sLeX attached to N-glycans and non-sulphated sLeX attached to extended core 1 O-glycans. These results indicate that MECA-79(-) /HECA-452(+) /NCC-ST-439(+) glycans, specifically, 6-sulpho- and non-sulphated sLeXs attached to core 2-branched O-glycans, expressed on HEV-like vessels in gastric MALT lymphoma function as L-selectin ligands and likely contribute to H. pylori-specific T cell recruitment in the progression of gastric MALT lymphoma.
authors: Department of Molecular Pathology, Shinshu University Graduate School of Medicine, Matsumoto, Japan. motokoba@shinshu-u.ac.jp
authors: Motohiro
authors: M
authors: Kobayashi
authors: Junya
authors: J
authors: Mitoma
authors: Hitomi
authors: H
authors: Hoshino
authors: Shin-Yi
authors: SY
authors: Yu
authors: Yasuyo
authors: Y
authors: Shimojo
authors: Kenichi
authors: K
authors: Suzawa
authors: Kay-Hooi
authors: KH
authors: Khoo
authors: Minoru
authors: M
authors: Fukuda
authors: Jun
authors: J
authors: Nakayama
mesh_terms: D000818:Animals; D016466:CHO Cells; D002448:Cell Adhesion; D006224:Cricetinae; D003412:Cricetulus; D005756:Gastritis / Q000378:metabolism / Q000473:pathology; D016481:Helicobacter Infections / Q000378:metabolism / Q000473:pathology; D016480:Helicobacter pylori; D006801:Humans; D007075:Immunoglobulin M / Q000378:metabolism; D019041:L-Selectin / Q000378:metabolism; D016256:Lewis X Antigen / Q000378:metabolism*; D008024:Ligands; D042601:Lymphatic Vessels / Q000378:metabolism* / Q000473:pathology; D008214:Lymphocytes / Q000378:metabolism; D018442:Lymphoma, B-Cell, Marginal Zone / Q000378:metabolism* / Q000473:pathology; D009363:Neoplasm Proteins / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism*; D000080506:Sialyl Lewis X Antigen; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology
chemical_list: D007075:Immunoglobulin M; D016256:Lewis X Antigen; D008024:Ligands; D009363:Neoplasm Proteins; D011134:Polysaccharides; D000080506:Sialyl Lewis X Antigen; D019041:L-Selectin