title: Purification of 6×His-tagged phycobiliprotein using zinc-decorated silica-coated magnetic nanoparticles.
abstract: Zinc-decorated silica-coated magnetic nanoparticles (ZnSiMNPs) were prepared by adsorbing zinc onto colloidal silica. These nanoparticles were used for the rapid purification of 6×His-tagged recombinant phycobiliprotein. The surface changes in the magnetic nanoparticles after zinc adsorption were characterized by transmission electron microscopy. The adsorption of the 6×His-tagged phycobiliprotein onto ZnSiMNPs in 10 mM PBS at 25°C was found to follow the Langmuir isotherm. ZnSiMNPs could be used to extract 6×His-tagged phycobiliprotein from lysate to single-band purity, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. No spectral variation was observed in the purified phycobiliprotein. Thus, ZnSiMNPs served as a useful tool for the magnetic separation and delivery of the 6×His-tagged phycobiliprotein.
authors: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China. cyjshandong@sohu.com
authors: Yingjie
authors: Y
authors: Chen
authors: Peng
authors: P
authors: Jiang
authors: Shaofang
authors: S
authors: Liu
authors: Hui
authors: H
authors: Zhao
authors: Yulin
authors: Y
authors: Cui
authors: Song
authors: S
authors: Qin
mesh_terms: D000327:Adsorption; D002846:Chromatography, Affinity / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D006639:Histidine / Q000737:chemistry / Q000302:isolation & purification*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D009842:Oligopeptides / Q000737:chemistry / Q000302:isolation & purification*; D010316:Particle Size; D052979:Phycobiliproteins / Q000737:chemistry / Q000302:isolation & purification*; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000302:isolation & purification*; D012822:Silicon Dioxide / Q000737:chemistry; D015032:Zinc / Q000737:chemistry
chemical_list: C471213:His-His-His-His-His-His; D058185:Magnetite Nanoparticles; D009842:Oligopeptides; D052979:Phycobiliproteins; D011993:Recombinant Fusion Proteins; D006639:Histidine; D012822:Silicon Dioxide; D015032:Zinc