title: Multilayer polymer microchip capillary array electrophoresis devices with integrated on-chip labeling for high-throughput protein analysis.
abstract: It is desirable to have inexpensive, high-throughput systems that integrate multiple sample analysis processes and procedures, for applications in biology, chemical analysis, drug discovery, and disease screening. In this paper, we demonstrate multilayer polymer microfluidic devices with integrated on-chip labeling and parallel electrophoretic separation of up to eight samples. Microchannels were distributed in two different layers and connected through interlayer through-holes in the middle layer. A single set of electrophoresis reservoirs and one fluorescent label reservoir address parallel analysis units for up to eight samples. Individual proteins and a mixture of cancer biomarkers have been successfully labeled on-chip and separated in parallel with this system. A detection limit of 600 ng/mL was obtained for heat shock protein 90. Our integrated on-chip labeling microdevices show great potential for low-cost, simplified, rapid, and high-throughput analysis.
authors: Department of Chemistry and Biochemistry, Brigham Young University, Provo, Utah 84602, United States.
authors: Ming
authors: M
authors: Yu
authors: Qingsong
authors: Q
authors: Wang
authors: James E
authors: JE
authors: Patterson
authors: Adam T
authors: AT
authors: Woolley
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis / Q000737:chemistry; D002417:Cattle; D046209:Electrophoresis, Microchip / Q000295:instrumentation*; D004867:Equipment Design; D005456:Fluorescent Dyes / Q000737:chemistry; D018841:HSP90 Heat-Shock Proteins / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D057230:Limit of Detection; D019904:Polymethyl Methacrylate / Q000737:chemistry*; D011506:Proteins / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification*; D012710:Serum Albumin, Bovine / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D013050:Spectrometry, Fluorescence; D013194:Staining and Labeling; D018511:Systems Integration
chemical_list: D015415:Biomarkers; D005456:Fluorescent Dyes; D018841:HSP90 Heat-Shock Proteins; D011506:Proteins; D012710:Serum Albumin, Bovine; D019904:Polymethyl Methacrylate