title: [Immunogenicity of protective antigen extracted from asporogenic recombinant strain Bacillus anthracis].
abstract: OBJECTIVE
  To study the ability of recombinant protective antigen (PA) to stimulate adaptive immune response in laboratory animals.
  
  
  METHODS
  Vaccine, recombinant, and reference strains of Bacillus anthracis were used in the study. Laboratory animals were immunized subcutaneously with two doses of antigenic preparation or one dose of B. anthracis strain. After inoculation with reference strain of B. anthracis, measurement of LD50 as well as indexes of immunity was performed by specified methods.
  
  
  RESULTS
  It was revealed that asporogenic recombinant strain has stable biological characteristics during passages in vitro and is effective producer of PA. Using 2-stage chromatography, highly purified protein was obtained. Experiments on different biomodels--BALB/c mice, guinea pigs, and rabbits--demonstrated high protective activity of PA obtained from asporogenic producer. Increase of immunity index was noted when EA1 protein from S-layer was added to preparation for immunization.
  
  
  CONCLUSIONS
  Immunity indexes determined in experiments on laboratory animals point to high protective efficacy of recombinant PA. Further studies of its interaction with macroorganism's innate and adaptive immunity systems are promising.
authors: N I
authors: NI
authors: Mikshis
authors: P Iu
authors: PIu
authors: Popova
authors: O M
authors: OM
authors: Kudriavtseva
authors: A Iu
authors: AIu
authors: Goncharova
authors: Iu A
authors: IuA
authors: Popov
authors: V V
authors: VV
authors: Kutyrev
mesh_terms: D056704:Adaptive Immunity / Q000187:drug effects; D000818:Animals; D000881:Anthrax / Q000235:genetics / Q000276:immunology* / Q000517:prevention & control*; D022122:Anthrax Vaccines / Q000235:genetics / Q000276:immunology / Q000494:pharmacology*; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology / Q000494:pharmacology*; D001408:Bacillus anthracis / Q000235:genetics / Q000276:immunology*; D001427:Bacterial Toxins / Q000235:genetics / Q000276:immunology / Q000494:pharmacology*; D006168:Guinea Pigs; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics / Q000276:immunology / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits
chemical_list: D022122:Anthrax Vaccines; D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D008562:Membrane Glycoproteins; C055764:S-layer proteins; C030325:anthrax toxin