title: Enhancement of the radiation effects by D-allose in head and neck cancer cells.
abstract: The aim of this study was to investigate the radiosensitizing potential of D-allose in human head and neck cancer cells. HSC-3 cells were treated with or without D-allose for 6 h and then irradiated (2-6 Gy). The combination of D-allose and radiation was more effective than either agent alone. The radiation enhancement ratios at the 37% survival level were 1.61 and 2.11 for 10 mM and 25 mM D-allose treatment, respectively. The combination of D-allose and radiation also reduced the cell proliferation in 3D culture experiments. Although the mRNA expression of TXNIP was not increased by radiation alone, combined use with D-allose markedly elevated TXNIP expression. The combination of D-allose and radiation significantly induced intracellular reactive oxygen species (ROS) and apoptosis compared to that induced by either agent alone. This study shows that D-allose enhances the effect of radiation, suggesting a potential clinical application of combination treatment with D-allose and radiation for head and neck cancer.
authors: Department of Otolaryngology, Faculty of Medicine, Kagawa University, Miki-cho, Kita-gun, Japan. hiro@med.kagawa-u.ac.jp
authors: Hiroshi
authors: H
authors: Hoshikawa
authors: Kanako
authors: K
authors: Indo
authors: Terushige
authors: T
authors: Mori
authors: Nozomu
authors: N
authors: Mori
mesh_terms: D017209:Apoptosis; D002352:Carrier Proteins / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival; D004305:Dose-Response Relationship, Drug; D005947:Glucose / Q000737:chemistry / Q000494:pharmacology*; D006258:Head and Neck Neoplasms / Q000188:drug therapy* / Q000532:radiotherapy*; D006801:Humans; D012333:RNA, Messenger / Q000378:metabolism; D017382:Reactive Oxygen Species; D013997:Time Factors; D016896:Treatment Outcome
chemical_list: D002352:Carrier Proteins; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; C486715:TXNIP protein, human; C002055:allose; D005947:Glucose