title: Heme oxygenase-1 transgenic overexpression did not prevent artery injury induced by electric stimulation and pressure overload in mice.
abstract: Heme oxygenase-1 (HO-1) shows multiple beneficial effects on cardiovascular diseases. However, the effect of HO-1 on the injury of artery has never been identified. In the present study, we established systemic HO-1 overexpression transgenic mice and investigated the effect of HO-1 on the injury of artery induced by electric stimulation and pressure-overload in transgenic mice. Artery injury was induced by electric stimulation and pressure overload. The contractive function, endothelium-dependent and -independent relaxation of arteries were measured through an isometric force transducer connected to a multichannel acquisition and analysis system. Western blot results showed that HO-1 protein level in transgenic mice arteries was significantly higher than that in wild type mice arteries, while no difference of HO-2 protein level in the arteries of transgenic and wild type mice. Arterial reendothelialization after electric injury was accelerated in transgenic mice. No significant difference in contractive function, endothelium-dependent and -independent relaxation of arteries was observed between wild type and transgenic mice at day 7 after electric injury and 4 weeks after pressure overload. We concluded that HO-1 overexpression accelerated the reendothelialization, but did not prevent the functional impairment of injured artery in mice.
authors: State-Province Key Laboratories of Biomedicine-Pharmaceutics of China, PR China. gaoxu6712@yahoo.com.cn
authors: Xu
authors: X
authors: Gao
authors: Hui-Bin
authors: HB
authors: Liu
authors: Ji-Chao
authors: JC
authors: Wu
authors: Jing
authors: J
authors: Liang
authors: Ling-Yun
authors: LY
authors: Zhou
authors: Ling-Ling
authors: LL
authors: Zhao
authors: Chang
authors: C
authors: Chen
authors: Yan
authors: Y
authors: Tong
authors: Dong-Mei
authors: DM
authors: Gong
authors: Osamu
authors: O
authors: Nakajima
authors: Bao-Feng
authors: BF
authors: Yang
authors: De-Li
authors: DL
authors: Dong
mesh_terms: D000818:Animals; D001158:Arteries / Q000293:injuries* / Q000378:metabolism*; D004558:Electric Stimulation / Q000009:adverse effects; D004730:Endothelium, Vascular / Q000378:metabolism; D015870:Gene Expression; D051547:Heme Oxygenase-1 / Q000235:genetics*; D051379:Mice; D008822:Mice, Transgenic; D011312:Pressure / Q000009:adverse effects*; D019076:Transgenes / Q000235:genetics*
chemical_list: D051547:Heme Oxygenase-1