title: Two modes of binding of DinI to RecA filament provide a new insight into the regulation of SOS response by DinI protein.
abstract: RecA protein plays a principal role in bacterial SOS response to DNA damage. The induction of the SOS response is well understood and involves the cleavage of the LexA repressor catalyzed by the RecA nucleoprotein filament. In contrast, our understanding of the regulation and termination of the SOS response is much more limited. RecX and DinI are two major regulators of RecA's ability to promote LexA cleavage and strand exchange reaction, and are believed to modulate its activity in ongoing SOS events. DinI's function in the SOS response remains controversial, since its interaction with the RecA filament is concentration dependent and may result in either stabilization or depolymerization of the filament. The 17 C-terminal residues of RecA modulate the interaction between DinI and RecA. We demonstrate that DinI binds to the active RecA filament in two distinct structural modes. In the first mode, DinI binds to the C-terminus of a RecA protomer. In the second mode, DinI resides deeply in the groove of the RecA filament, with its negatively charged C-terminal helix proximal to the L2 loop of RecA. The deletion of the 17 C-terminal residues of RecA favors the second mode of binding. We suggest that the negatively charged C-terminus of RecA prevents DinI from entering the groove and protects the RecA filament from depolymerization. Polymorphic binding of DinI to RecA filaments implies an even more complex role of DinI in the bacterial SOS response.
authors: Department of Biochemistry and Molecular Genetics, University of Virginia, Jordan Hall 6007, 1340 Jefferson Park Avenue, Charlottesville, VA 22908, USA. galkin@virginia.edu
authors: Vitold E
authors: VE
authors: Galkin
authors: Rachel L
authors: RL
authors: Britt
authors: Lukas B
authors: LB
authors: Bane
authors: Xiong
authors: X
authors: Yu
authors: Michael M
authors: MM
authors: Cox
authors: Edward H
authors: EH
authors: Egelman
mesh_terms: D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation; D011938:Rec A Recombinases / Q000737:chemistry / Q000378:metabolism*; D013014:SOS Response, Genetics
chemical_list: C422844:DinI protein, E coli; D029968:Escherichia coli Proteins; D011938:Rec A Recombinases