title: Evaluation of different cryoprotectants (CPAs) in boar semen cryopreservation.
abstract: This study was performed to evaluate the use of dimethylacetamide (DMA) and dimethyl sulfoxide (DMSO) in boar sperm cryopreservation. Semen from eight boars was cryopreserved following treatment with 3, 5, and 7% DMA and DMSO, and 3% glycerol (control). After thawing, sperm conventional parameters and membrane integrities were evaluated. There were no significant differences among different DMA concentrations in all evaluations. Membrane intactness were higher in 5% and 7% DMSO than 3% DMSO (P<0.05). Sperm motility of 5% DMSO was lower than that of 3% glycerol (P<0.005), and membrane intactness were lower in 5% DMA and DMSO than 3% glycerol (P<0.05). DMA and DMSO didn't improve sperm quality and glycerol remains the most useful for boar sperm cryopreservation.
authors: Department of Veterinary Obstetrics and Theriogenology, College of Veterinary Medicine, Chonbuk National University, Jeonju, Jeonbuk 561-756, Republic of Korea.
authors: Suhee
authors: S
authors: Kim
authors: Young-Jun
authors: YJ
authors: Lee
authors: Dong-Beom
authors: DB
authors: Ji
authors: Yong-Jun
authors: YJ
authors: Kim
mesh_terms: D000081:Acetamides / Q000494:pharmacology; D000818:Animals; D002462:Cell Membrane / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005434:Flow Cytometry / Q000662:veterinary; D008297:Male; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013081:Sperm Motility / Q000502:physiology; D013094:Spermatozoa; D018709:Statistics, Nonparametric; D013552:Swine
chemical_list: D000081:Acetamides; D003451:Cryoprotective Agents; C013959:dimethylacetamide; D004121:Dimethyl Sulfoxide