title: Adhesive interactions between cells and biotinylated phospholipid vesicles in alginate: towards new responsive biomaterials.
abstract: Creating tissue-mimetic biomaterials able to deliver bioactive compounds after receipt of a remote and non-invasive trigger has so far proved to be challenging. The possible applications of such "smart" biomaterials are vast, ranging from subcutaneous drug delivery to tissue engineering. Self-assembled phospholipid vesicles (liposomes) have the ability to deliver both hydrophilic and hydrophobic drugs, and controlling interactions between functionalized vesicles and cells within biomaterials is an important step for targeted drug delivery to cells. We report an investigation of the interactions between thermally-sensitive and biotin-coated dipalmitoyl phosphatidylcholine vesicles and 3T3 fibroblast cells. The stability of these vesicles under physiological conditions was assessed and their interaction with the cell membranes of fibroblasts in media and alginate/fibronectin mixtures was studied. Stable vesicle-cell aggregates were formed in fluid matrices, and could be a model system for improving the delivery of remotely released drugs within vesicle-containing biomaterials.
authors: School of Chemistry and Manchester Interdisciplinary Biocentre, University of Manchester, 131 Princess Street, Manchester M1 7DN, UK.
authors: Felicity
authors: F
authors: de Cogan
authors: Julie E
authors: JE
authors: Gough
authors: Simon J
authors: SJ
authors: Webb
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry; D016475:3T3 Cells; D000464:Alginates / Q000737:chemistry*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001710:Biotin / Q000737:chemistry; D019921:Biotinylation; D002462:Cell Membrane / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D005353:Fibronectins / Q000737:chemistry; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D051379:Mice; D010743:Phospholipids / Q000737:chemistry*; D012235:Rhodamines / Q000737:chemistry
chemical_list: D000464:Alginates; D001672:Biocompatible Materials; D005353:Fibronectins; D006603:Hexuronic Acids; D010743:Phospholipids; D012235:Rhodamines; D015060:1,2-Dipalmitoylphosphatidylcholine; D001710:Biotin; D020723:Glucuronic Acid; D016650:Fluorescein-5-isothiocyanate