title: Differential expression of microRNAs in cardiac myocytes compared to undifferentiated P19 cells.
abstract: microRNA (miRNA) expression is tightly controlled in a tissue-specific and developmental stage-specific manner; some are highly and specifically expressed in cardiovascular tissues. miRNA expression profiling, using miRNA microarrays facilitates studying the biological function of miRNAs. We investigated changes in miRNA expression profiles during differentiation of P19 cells into cardiac myocytes in order to elucidate the mechanisms of heart development. The morphology of P19 cells during differentiation was observed using an inverted microscope. Western blot analysis was performed to detect cardiac troponin I (cTnI) expression. Total RNA was extracted from P19 cells for microarray and real-time quantitative reverse transcription-polymerase chain reaction (real-time qRT-PCR) analyses to determine the miRNA expression profile. The miRNA microarray revealed differential expression of 49 miRNAs, of which 26 were down-regulated and 23 were up-regulated in differentiated cardiac myocytes, compared to normal P19 cells. This was confirmed by real-time qRT-PCR. We also utilized target prediction analysis to identify gene targets. Some miRNAs may have important roles in cardiac development and congenital heart defects (CHDs). Further analysis of miRNA function to confirm their target genes during cardiac development will determine the potential for novel miRNA-based therapeutic strategies.
authors: Department of Cardiology, First Affiliated Hospital of Nanjing Medical University, 210029 Nanjing, PR China.
authors: De-Liang
authors: DL
authors: Hu
authors: Yao-Qiu
authors: YQ
authors: Liu
authors: Fu-Kun
authors: FK
authors: Chen
authors: Yan-Hui
authors: YH
authors: Sheng
authors: Rong
authors: R
authors: Yang
authors: Xiang-Qing
authors: XQ
authors: Kong
authors: Ke-Jiang
authors: KJ
authors: Cao
authors: Jin-Song
authors: JS
authors: Zhang
authors: Ling-Mei
authors: LM
authors: Qian
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D045744:Cell Line, Tumor; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D006321:Heart / Q000254:growth & development*; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D019210:Troponin I / Q000235:genetics
chemical_list: D035683:MicroRNAs; D019210:Troponin I