title: Characterization of RISC-associated adenoviral small RNAs.
abstract: RNA interference (RNAi) plays novel roles in both host antiviral defense and viral replication. It has been shown that some viruses can exploit the RNAi machinery for their own benefit by encoding for their own viral small RNAs. Here we present a collection of methods to study adenoviral small RNAs, specifically a method for immunopurification of RNA-induced silencing complex (RISC) and a biochemical assay for the activity of purified RISC associated with adenoviral small RNAs.
authors: Department of Medical Biochemistry and Microbiology, Uppsala Biomedical Center (BMC), Uppsala, Sweden.
authors: Ning
authors: N
authors: Xu
authors: Göran
authors: G
authors: Akusjärvi
mesh_terms: D000256:Adenoviridae / Q000235:genetics* / Q000502:physiology; D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D020868:Gene Silencing; D047468:Immunoprecipitation; D007553:Isotope Labeling; D034741:RNA, Small Interfering / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D012367:RNA, Viral / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D034741:RNA, Small Interfering; D012367:RNA, Viral