title: Hemoglobin loaded polymeric nanoparticles: preparation and characterizations.
abstract: In the present work polymeric nanoparticles based on Poly (maleic anhydride-alt-butyl vinyl ether) 5% grafted with m-PEG (2000) and 95% grafted with 2-methoxyethanol (VAM41-PEG) were loaded with human hemoglobin (Hb) and characterized from a physicochemical point of view. The assessment of structural and functional features of the loaded Hb was performed and the effect of the introduction of different reducing agents as aimed at minimizing Hb oxidation during the nanoparticles formulation process, was also investigated. Nanoparticles possessing an average diameter of 138±10 nm and physicochemical features suitable for this kind of application were successfully obtained. Although the oxidation of the protein was not avoided during its loading into nanoparticles, the presence of acidic moieties in the polymeric structure is proposed to be directly involved in the protein inactivation mechanism.
authors: Laboratory of Bioactive Polymeric Materials for Biomedical and Environmental Application (BIOlab) UdR-INSTM Consortium Reference Center, University of Pisa, Via Livornese 1291, 56122 San Piero a Grado, Pisa, Italy.
authors: Alberto
authors: A
authors: Dessy
authors: Anna M
authors: AM
authors: Piras
authors: Giorgio
authors: G
authors: Schirò
authors: Matteo
authors: M
authors: Levantino
authors: Antonio
authors: A
authors: Cupane
authors: Federica
authors: F
authors: Chiellini
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D001802:Blood Substitutes / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry; D004987:Ethers / Q000737:chemistry; D005026:Ethylene Glycols / Q000737:chemistry; D006454:Hemoglobins / Q000737:chemistry / Q000648:ultrastructure; D006801:Humans; D008299:Maleic Anhydrides / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry; D014753:Vinyl Compounds / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D001802:Blood Substitutes; D004337:Drug Carriers; D004987:Ethers; D005026:Ethylene Glycols; D006454:Hemoglobins; D008299:Maleic Anhydrides; D014753:Vinyl Compounds; C521980:butyl vinyl ether; D011092:Polyethylene Glycols; C005219:methyl cellosolve