title: Loss of the extraproteasomal ubiquitin receptor Rings lost impairs ring canal growth in Drosophila oogenesis.
abstract: In Drosophila melanogaster oogenesis, there are 16 germline cells that form a cyst and stay connected to each other by ring canals. Ring canals allow the cytoplasmic transport of proteins, messenger ribonucleic acids, and yolk components from the nurse cells into the oocyte. In this paper, we describe the protein Rings lost (Rngo) and show that it is required for ring canal growth in germline cysts. rngo is an essential gene, and germline clones of a rngo-null allele show defects in ovary development, including mislocalization of ring canal proteins and fusion of germline cells. Rngo appears to be a ubiquitin receptor that possesses a ubiquitin-like domain, a ubiquitin-associated domain, and a retroviral-like aspartate protease (RVP) domain. Rngo binds to ubiquitin and to the 26S proteasome and colocalizes with both in germline cells, and its RVP domain is required for dimerization of Rngo and for its function in vivo. Our results thus show, for the first time, a function for a ubiquitin receptor in Drosophila development.
authors: Abteilung Stammzellbiologie, Forschungszentrum der Deutschen Forschungsgemeinschaft für Molekularphysiologie des Gehirns, Georg-August-Universität Göttingen, 37077 Göttingen, Germany.
authors: Tobias
authors: T
authors: Morawe
authors: Mona
authors: M
authors: Honemann-Capito
authors: Walter
authors: W
authors: von Stein
authors: Andreas
authors: A
authors: Wodarz
mesh_terms: D000818:Animals; D004331:Drosophila melanogaster / Q000254:growth & development*; D009866:Oogenesis / Q000235:genetics*; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism*; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics / Q000378:metabolism*
chemical_list: D018160:Receptors, Cytoplasmic and Nuclear; D046988:Proteasome Endopeptidase Complex