title: TaqMan assays for the genetic identification of delta smelt (Hypomesus transpacificus) and wakasagi smelt (Hypomesus nipponensis).
abstract: We have developed species-specific TaqMan assays for two California fish species, the threatened delta smelt (Hypomesus transpacificus) and the introduced wakasagi smelt (Hypomesus nipponensis). The assays are capable of correctly identifying each species with 100% accuracy, with no cross-species amplification. We anticipate these assays will prove useful for future scientific studies requiring genetic species identification (e.g. predation of smelt) or monitoring (e.g. detection of delta smelt near water diversions).
authors: Department of Animal Science, University of California, Davis, One Shields Ave, Davis, CA 95616, USA. mrbaerwald@ucdavis.edu
authors: Melinda R
authors: MR
authors: Baerwald
authors: Gregg
authors: G
authors: Schumer
authors: Brian M
authors: BM
authors: Schreier
authors: Bernie
authors: B
authors: May
mesh_terms: D000818:Animals; D002140:California; D017931:DNA Primers / Q000235:genetics; D056727:Endangered Species; D014644:Genetic Variation; D058865:Introduced Species; D036441:Osmeriformes / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D013045:Species Specificity; D019914:Taq Polymerase
chemical_list: D017931:DNA Primers; D019914:Taq Polymerase