title: Temperature affects brain and pituitary gene expression related to reproduction and growth in the male blue gouramis, Trichogaster trichopterus.
abstract: This study examined the effect of temperature on reproduction and growth-related factors in blue gourami males under nonreproductive and reproductive conditions. Males that were maintained under nonreproductive conditions did not build nest and the gonado-somatic index (% GSI) was significantly higher in fish maintained at 27°C compared with fish maintained at 23°C. The relative mRNA levels of brain gonadotropin-releasing hormone 3 (GnRH3), pituitary adenylate cyclase-activating polypeptide (PACAP), insulin-like growth factor-1(IGF-1), pituitary β-luteinizing hormone (βLH), and prolactin were significantly higher when the fish were maintained at 27°C than at 23°C or 31°C. β-Follicle-stimulating hormone (βFSH) mRNA levels were significantly lower when maintained at 31°C than at the other temperatures. Nests were observed only in males under reproductive conditions. In these fish, higher mRNA levels of GnRH3, PACAP, βFSH, βLH and prolactin were detected at 27°C, and higher mRNA levels of IGF-1 were detected at 23°C, when compared with other temperature of maintenance or with fish that did not build nest. In conclusion, we propose that temperature has more effect on the transcription of genes, associated with reproduction, than on those pertaining to growth.
authors: School of Science and Technology, Tel-Hai Academic College, Upper Galilee, Israel.
authors: Dalia
authors: D
authors: David
authors: Gad
authors: G
authors: Degani
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D005786:Gene Expression Regulation; D007987:Gonadotropin-Releasing Hormone / Q000235:genetics; D007334:Insulin-Like Growth Factor I / Q000235:genetics; D037101:Luteinizing Hormone, beta Subunit / Q000235:genetics; D008297:Male; D010473:Perciformes / Q000235:genetics* / Q000254:growth & development / Q000502:physiology*; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide / Q000235:genetics; D010902:Pituitary Gland / Q000378:metabolism*; D011388:Prolactin / Q000235:genetics; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives; D012098:Reproduction / Q000235:genetics; D013696:Temperature
chemical_list: D037101:Luteinizing Hormone, beta Subunit; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; C448022:gonadotropin-releasing hormone-III; D007987:Gonadotropin-Releasing Hormone; D007334:Insulin-Like Growth Factor I; D011388:Prolactin; D011761:Pyrrolidonecarboxylic Acid