title: Experimental uptake and retention of pathogenic and nonpathogenic Vibrio parahaemolyticus in two species of clams: Ruditapes decussatus and Ruditapes philippinarum.
abstract: OBJECTIVE
  To describe uptake and retention of pathogenic and nonpathogenic Vibrio parahaemolyticus in Ruditapes decussatus and Ruditapes philippinarum.
  
  
  RESULTS
  Ruditapes decussatus accumulated greater concentrations of pathogenic and nonpathogenic V. parahaemolyticus than R. philippinarum. These concentrations decreased earlier in R. decussatus. Nonpathogenic V. parahaemolyticus reached higher concentrations (approx. 1 log CFU g⁻¹ in tissues of R. decussatus and more than 1 log CFU g⁻¹ in R. philippinarum) than pathogenic V. parahaemolyticus at similar times. It also persisted longer in both species of clams (72 h in R. decussatus and 96 h in R. philippinarum), while pathogenic V. parahaemolyticus persisted 48 h in R. decussatus and 72 h in R. philippinarum.
  
  
  CONCLUSIONS
  Ruditapes decussatus incorporated both isolates of V. parahaemolyticus faster than R. philippinarum and it eliminated both isolates earlier than R. philippinarum. Under same conditions, nonpathogenic V. parahaemolyticus might survive better than pathogenic V. parahaemolyticus within both species of clam.
  
  
  CONCLUSIONS
  Pathogenic V. parahaemolyticus is an important cause of foodborne illnesses. This study shows it may be possible to use nonpathogenic V. parahaemolyticus to perform experimentation to evaluate bacterial evolution in clams, developing an in vivo model useful for risk analysis.
authors: IRTA-SCR, Sant Carles de la Ràpita, Spain.
authors: C
authors: C
authors: Lopez-Joven
authors: I
authors: I
authors: de Blas
authors: I
authors: I
authors: Ruiz-Zarzuela
authors: M D
authors: MD
authors: Furones
authors: A
authors: A
authors: Roque
mesh_terms: D000818:Animals; D049872:Bivalvia / Q000382:microbiology*; D012758:Shellfish / Q000382:microbiology*; D014736:Vibrio parahaemolyticus / Q000302:isolation & purification* / Q000472:pathogenicity