title: Molecular cloning, expression, and regulation of goose leptin receptor gene in adipocytes.
abstract: A full-length cDNA encoding the goose (Anser anser) leptin receptor (LEPR) was cloned and sequenced. The goose LEPR gene encodes a 1,156-amino acid protein containing a signal peptide, a single transmembrane domain and specific motifs involving putative leptin-binding and signal transduction. The deduced goose LEPR protein shows more than 90% identity to duck and 75% identity to chicken and turkey. Quantitative real-time analysis reveals that the goose LEPR is predominantly expressed in brain. The expression of LEPR in goose adipocytes can be up-regulated by oleic acid in vitro. Moreover, the expression levels of genes, which have been demonstrated to be related to adipocyte differentiation, are down-regulated in LEPR-knockdown adipocytes, indicating LEPR's potential role in adipocyte differentiation in goose.
authors: College of Animal Sciences, Zhejiang University, 258 Kaixuan Road, Hangzhou, Zhejiang 310029, China.
authors: Fang
authors: F
authors: Wang
authors: Lizhi
authors: L
authors: Lu
authors: Hao
authors: H
authors: Yuan
authors: Yong
authors: Y
authors: Tian
authors: Jinjun
authors: J
authors: Li
authors: Junda
authors: J
authors: Shen
authors: Zhengrong
authors: Z
authors: Tao
authors: Yan
authors: Y
authors: Fu
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D030161:Avian Proteins / Q000235:genetics*; D001921:Brain / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D005777:Geese / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D008969:Molecular Sequence Data; D019301:Oleic Acid / Q000494:pharmacology; D034622:RNA Interference; D054411:Receptors, Leptin / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D015854:Up-Regulation / Q000187:drug effects / Q000235:genetics
chemical_list: D030161:Avian Proteins; D018076:DNA, Complementary; D054411:Receptors, Leptin; D019301:Oleic Acid