title: Clonal evolution of adult T-cell leukemia/lymphoma takes place in the lymph nodes.
abstract: Adult T-cell leukemia/lymphoma (ATLL) is the neoplasm caused by human T-cell leukemia virus type 1 (HTLV-1). We performed oligo-array comparative genomic hybridization (CGH) against paired samples comprising peripheral blood (PB) and lymph node (LN) samples from 13 patients with acute ATLL. We found that the genome profiles of the PB frequently differed from those of the LN samples. The results showed that 9 of 13 cases investigated had a log2 ratio imbalance among chromosomes, and that chromosome imbalances were more frequent in LN samples. Detailed analysis revealed that the imbalances were likely caused by the presence of multiple subclones in the LN samples. Five of 13 cases showed homozygous loss regions in PB samples, which were not found in the LN samples, indicating that tumors in the PB were derived from LN subclones in most cases. Southern blot analysis of TCRγ showed that these multiple subclones originated from a common clone. We concluded that in many ATLL cases, multiple subclones in the LNs originate from a common clone, and that a selected subclone among the LN subclones appears in the PB.
authors: Division of Molecular Medicine, Aichi Cancer Center Research Institute, 1-1 Kanokoden, Chikusa-ku, Nagoya, Aichi, Japan.
authors: Akira
authors: A
authors: Umino
authors: Masao
authors: M
authors: Nakagawa
authors: Atae
authors: A
authors: Utsunomiya
authors: Kunihiro
authors: K
authors: Tsukasaki
authors: Naoya
authors: N
authors: Taira
authors: Naoyuki
authors: N
authors: Katayama
authors: Masao
authors: M
authors: Seto
mesh_terms: D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000821:virology; D045744:Cell Line, Tumor; D019070:Cell Lineage / Q000235:genetics; D002869:Chromosome Aberrations; D055028:Comparative Genomic Hybridization; D020869:Gene Expression Profiling; D015334:Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor; D015368:Human T-lymphotropic virus 1 / Q000235:genetics; D006801:Humans; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000097:blood / Q000235:genetics* / Q000276:immunology / Q000821:virology*; D008198:Lymph Nodes / Q000276:immunology / Q000821:virology; D016662:Virus Integration / Q000235:genetics