title: A shorter interval between irradiation of recipient testis and germ cell transplantation is detrimental to recovery of fertility in rams.
abstract: The objective of the current study was to identify an optimal time period for donor cell transplantation after irradiation in sheep. The testes of recipient rams were treated with a single dose of 15 Gray (Gy) irradiation followed by germ cell transplantation either 3 or 6 weeks later. Transplantation of donor cells at 6 weeks after irradiation resulted in production of donor sperm by all five recipient rams compared with 4 of 11 rams transplanted at 3 weeks. Rams transplanted 3 weeks post-irradiation appeared to show reduced libido and fertility. Two rams produced sperm with low motility (< 20%) and two other rams were azoospermic. More than 1 year after cell transfer, there were heavy infiltrates of CD45-positive cells and more fibrous tissue in 9 of 14 recipient testes (seven rams) that received cells 3 weeks after irradiation. Taken together, these results suggest that the interval between irradiation of recipients and germ cell transplantation affects the success rate of the procedure, with a 6-week interval preferable. The elevated inflammatory/immune reaction may be responsible, at least in part, for the reduced fertility and low libido observed in the rams that received cells 3 weeks post-irradiation.
authors: CSIRO Food Futures National Research Flagship, Sydney, NSW, Australia. Muren.Herrid@csiro.au
authors: M
authors: M
authors: Herrid
authors: R
authors: R
authors: Davey
authors: S
authors: S
authors: Stockwell
authors: J
authors: J
authors: Olejnik
authors: S
authors: S
authors: Schmoelzl
authors: N
authors: N
authors: Suchowerska
authors: M
authors: M
authors: Jackson
authors: M
authors: M
authors: Holland
authors: J R
authors: JR
authors: Hill
mesh_terms: D000818:Animals; D004247:DNA / Q000378:metabolism; D004542:Ejaculation; D008297:Male; D012661:Semen / Q000378:metabolism; D012756:Sheep; D013094:Spermatozoa / Q000637:transplantation*; D013737:Testis / Q000276:immunology / Q000637:transplantation*
chemical_list: D004247:DNA