title: Germinal center-specific protein human germinal center associated lymphoma directly interacts with both myosin and actin and increases the binding of myosin to actin.
abstract: Human germinal center associated lymphoma (HGAL) is a germinal center-specific gene whose expression correlates with a favorable prognosis in patients with diffuse large B-cell and classic Hodgkin lymphomas. HGAL is involved in negative regulation of lymphocyte motility. The movement of lymphocytes is directly driven by actin polymerization and actin-myosin interactions. We demonstrate that HGAL interacts directly and independently with both actin and myosin and delineate the HGAL and myosin domains responsible for the interaction. Furthermore, we show that HGAL increases the binding of myosin to F-actin and inhibits the ability of myosin to translocate actin by reducing the maximal velocity of myosin head/actin movement. No effects of HGAL on actomyosin ATPase activity and the rate of actin polymerization from G-actin to F-actin were observed. These findings reveal a new mechanism underlying the inhibitory effects of germinal center-specific HGAL protein on lymphocyte and lymphoma cell motility.
authors: Division of Oncology-Hematology, Department of Medicine, Sylvester Comprehensive Cancer Center, University of Miami, FL 33136, USA.
authors: Xiaoqing
authors: X
authors: Lu
authors: Katarzyna
authors: K
authors: Kazmierczak
authors: Xiaoyu
authors: X
authors: Jiang
authors: Michelle
authors: M
authors: Jones
authors: James
authors: J
authors: Watt
authors: David M
authors: DM
authors: Helfman
authors: Jeffrey R
authors: JR
authors: Moore
authors: Danuta
authors: D
authors: Szczesna-Cordary
authors: Izidore S
authors: IS
authors: Lossos
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D018858:Germinal Center / Q000378:metabolism*; D006689:Hodgkin Disease / Q000378:metabolism*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D016403:Lymphoma, Large B-Cell, Diffuse / Q000378:metabolism*; D008840:Microfilament Proteins; D009218:Myosins / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D011817:Rabbits; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D000199:Actins; C476824:GCSAM protein, human; D047908:Intracellular Signaling Peptides and Proteins; D008840:Microfilament Proteins; D009363:Neoplasm Proteins; D011994:Recombinant Proteins; D009218:Myosins