title: [Chemical constituents from Callicarpa nudiflora and their hemostatic activity].
abstract: OBJECTIVE
  To study the hemostatic effect of chemical constituents from Callicarpa nudiflora.
  
  
  METHODS
  The chemical constituents were isolated and purified via silica gel and Sephadex LH-20 column chromatography. Their structures were determined on the basis of spectral analysis. prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT) and fibrinogen (FIB) of the constituents rabbit blood samples were tested with rabbit blood in vitro.
  
  
  RESULTS
  Eleven compounds were isolated and identified as two diterpenens: 7alpha-hydroxy sandaracopimaric acid (1), 16, 17-dihydroxy-3-oxophyllocladane (2). Two phenoic glycosides: acteoside (3), samioside(4). Three triterpenes: 2alpha, 3alpha, 24-trihydroxy-ursa-12-en-28-oic acid (5), 2alpha, 3alpha, 19alpha-trihydroxyursa-12-en-28-oic acid-28-0-beta-D-glucopyranosyl ester (6), and 2alpha, 3alpha, 19alpha, 23-tetrahydroxy-ursa-12-en-28-oic acid-28-0-beta-D-glucopyranosyl ester (7). Four flavones: rhamnazin (8), 5-Hydroxy-3, 7, 4'-trimethoxy-flavone (9) , 5-Hydroxy-3, 7, 3', 4'-tetramethoxyflavone (10), and luteoloside (11). All Compounds cannot significantly shorten the PT (P < 0.01), compounds 3, 4, 7, 10 can remarkedly increase APTT (P < 0.01), compound 5 can prolong the T( P < 0.01) obviously, and compound 8 can significantly increase the contents of FIB (P < 0.01).
  
  
  CONCLUSIONS
  Compounds 2, 4 and 10 were isolated from this genus for the first time, and compounds 1, 3, 5, 6, 7 and 9 had been isolated from this plant for the first time. The hemostatic effect of C. nudiflora may be related to the activation of the intrinsic blood coagulation system.
authors: Department of Phytochemistry, China Pharmaceutical University, Nanjing 210009, China.
authors: Jie
authors: J
authors: Zhang
authors: Baoquan
authors: B
authors: Li
authors: Feng
authors: F
authors: Feng
authors: Yuping
authors: Y
authors: Tang
authors: Wenyuan
authors: W
authors: Liu
mesh_terms: D000818:Animals; D001779:Blood Coagulation Factors / Q000378:metabolism; D032408:Callicarpa / Q000737:chemistry*; D006487:Hemostasis / Q000187:drug effects*; D008297:Male; D009930:Organic Chemicals / Q000032:analysis* / Q000494:pharmacology*; D011817:Rabbits
chemical_list: D001779:Blood Coagulation Factors; D009930:Organic Chemicals