title: Adaptation of Lactobacillus plantarum IMDO 130201, a wheat sourdough isolate, to growth in wheat sourdough simulation medium at different pH values through differential gene expression.
abstract: Sourdough is a very competitive and challenging environment for microorganisms. Usually, a stable microbiota composed of lactic acid bacteria (LAB) and yeasts dominates this ecosystem. Although sourdough is rich in carbohydrates, thus providing an ideal environment for microorganisms to grow, its low pH presents a particular challenge. The nature of the adaptation to this low pH was investigated for Lactobacillus plantarum IMDO 130201, an isolate from a laboratory wheat sourdough fermentation. Batch fermentations were carried out in wheat sourdough simulation medium, and total RNA was isolated from mid-exponential-growth-phase cultures, followed by differential gene expression analysis using a LAB functional gene microarray. At low pH values, an increased expression of genes involved in peptide and amino acid metabolism was found as well as that of genes involved in plantaricin production and lipoteichoic acid biosynthesis. The results highlight cellular mechanisms that allow L. plantarum to function at a low environmental pH.
authors: Research Group of Industrial Microbiology and Food Biotechnology (IMDO), Faculty of Sciences and Bio-engineering Sciences, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussels, Belgium.
authors: Gino
authors: G
authors: Vrancken
authors: Luc
authors: L
authors: De Vuyst
authors: Tom
authors: T
authors: Rimaux
authors: Joke
authors: J
authors: Allemeersch
authors: Stefan
authors: S
authors: Weckx
mesh_terms: D000222:Adaptation, Physiological; D003470:Culture Media / Q000737:chemistry*; D005516:Food Microbiology; D015870:Gene Expression; D020869:Gene Expression Profiling; D006863:Hydrogen-Ion Concentration; D048191:Lactiplantibacillus plantarum / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D046228:Microarray Analysis; D014908:Triticum / Q000378:metabolism
chemical_list: D003470:Culture Media