title: Activity of FEN1 endonuclease on nucleosome substrates is dependent upon DNA sequence but not flap orientation.
abstract: We demonstrated previously that human FEN1 endonuclease, an enzyme involved in excising single-stranded DNA flaps that arise during Okazaki fragment processing and base excision repair, cleaves model flap substrates assembled into nucleosomes. Here we explore the effect of flap orientation with respect to the surface of the histone octamer on nucleosome structure and FEN1 activity in vitro. We find that orienting the flap substrate toward the histone octamer does not significantly alter the rotational orientation of two different nucleosome positioning sequences on the surface of the histone octamer but does cause minor perturbation of nucleosome structure. Surprisingly, flaps oriented toward the nucleosome surface are accessible to FEN1 cleavage in nucleosomes containing the Xenopus 5S positioning sequence. In contrast, neither flaps oriented toward nor away from the nucleosome surface are cleaved by the enzyme in nucleosomes containing the high-affinity 601 nucleosome positioning sequence. The data are consistent with a model in which sequence-dependent motility of DNA on the nucleosome is a major determinant of FEN1 activity. The implications of these findings for the activity of FEN1 in vivo are discussed.
authors: Department of Biochemistry and Biophysics, University of Rochester, Rochester, New York 14642, USA.
authors: Indu
authors: I
authors: Jagannathan
authors: Sharon
authors: S
authors: Pepenella
authors: Jeffrey J
authors: JJ
authors: Hayes
mesh_terms: D000818:Animals; D004247:DNA / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D045585:Flap Endonucleases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006801:Humans; D009707:Nucleosomes / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D013379:Substrate Specificity; D014981:Xenopus
chemical_list: D009707:Nucleosomes; C022262:Okazaki fragments; D004247:DNA; D045585:Flap Endonucleases; C468317:FEN1 protein, human