title: Imaging mass spectrometry for lipidomics.
abstract: Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is a powerful tool that enables the simultaneous detection and identification of biomolecules in analytes. MALDI-imaging mass spectrometry (MALDI-IMS) is a two-dimensional MALDI-MS technique used to visualize the spatial distribution of biomolecules without extraction, purification, separation, or labeling of biological samples. This technique can reveal the distribution of hundreds of ion signals in a single measurement and also helps in understanding the cellular profile of the biological system. MALDI-IMS has already revealed the characteristic distribution of several kinds of lipids in various tissues. The versatility of MALDI-IMS has opened a new frontier in several fields, especially in lipidomics. In this review, we describe the methodology and applications of MALDI-IMS to biological samples.
authors: Department of Molecular Anatomy, Hamamatsu University School of Medicine, Shizuoka, Japan.
authors: Naoko
authors: N
authors: Goto-Inoue
authors: Takahiro
authors: T
authors: Hayasaka
authors: Nobuhiro
authors: N
authors: Zaima
authors: Mitsutoshi
authors: M
authors: Setou
mesh_terms: D053000:Analytic Sample Preparation Methods; D000818:Animals; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods*; D050356:Lipid Metabolism; D008055:Lipids / Q000032:analysis* / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D008055:Lipids