title: Detection of collagens in brain tumors based on FTIR imaging and chemometrics.
abstract: Fourier transform infrared (FTIR) imaging has been used as a molecular histopathology tool on brain tissue sections after intracranial implantation and development of glioma tumors. Healthy brain tissue (contralateral lobe) as well as solid and diffuse tumor tissues were compared for their collagen contents. IR spectra were extracted from IR images for determining the secondary structure of protein contents and compared to pure product spectra of collagens (types I, III, IV, V, and VI). Multivariate statistical analyses of variance and correspondence factorial analysis were performed to differentiate healthy and tumor brain tissues as well as their classification according to their secondary structure profiles. Secondary structure profiles revealed that no collagen was present in healthy tissues; they are also significantly different from solid and diffuse tumors (p < 0.05). Solid and diffuse tumors could be discriminated with respect to the secondary structure profile of fibrillar and non-fibrillar collagens, respectively. We can thus propose to develop FTIR imaging for histopathology examination of tumors on the basis of collagen contents.
authors: Université de Bordeaux, CNRS UMR 5248, B8 Avenue des Facultés, 33402 Talence Cedex, France.
authors: Razia
authors: R
authors: Noreen
authors: Chia-Chi
authors: CC
authors: Chien
authors: Maylis
authors: M
authors: Delugin
authors: Seydou
authors: S
authors: Yao
authors: Raphael
authors: R
authors: Pineau
authors: Yeukuang
authors: Y
authors: Hwu
authors: Michel
authors: M
authors: Moenner
authors: Cyril
authors: C
authors: Petibois
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000737:chemistry* / Q000000981:diagnostic imaging* / Q000473:pathology; D045744:Cell Line, Tumor; D003094:Collagen / Q000032:analysis* / Q000737:chemistry; D005910:Glioma / Q000737:chemistry* / Q000000981:diagnostic imaging* / Q000473:pathology; D008297:Male; D051379:Mice; D017433:Protein Structure, Secondary; D011859:Radiography; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D003094:Collagen