title: Antibody-binding epitope differences in the nucleoprotein of avian and mammalian influenza A viruses.
abstract: Abstract Influenza virus nucleoprotein (NP) binds to the viral genome RNA and forms the internal ribonucleoprotein complex of the virus particle. Avian and human influenza virus NP have characteristic differences at several amino acid positions. It is not known whether any of these differences can be recognized by antibodies. In the present study five monoclonal antibodies (MAbs) were produced against NP of A/Duck/Novosibirsk/56/05 (H5N1) influenza virus. Two MAbs discerned human and avian influenza strains on ELISA testing. The NP expressed in a prokaryotic system was used for the analysis of site-specific mutants carrying amino acid substitutions in the relevant positions. Amino acid residues in positions 100 and 101 were shown to be recognized by the MAbs. The residue in position 100 is host-specific, and its recognition by the MAb 2E6 may be useful for the differentiation of human and avian viruses. The data are discussed in view of the effects of amino acid substitutions in influenza virus NP affecting both host range and antibody-binding specificity.
authors: D.I. Ivanovsky Institute of Virology, Moscow, Russia.
authors: Natalia L
authors: NL
authors: Varich
authors: Galina K
authors: GK
authors: Sadykova
authors: Alexei G
authors: AG
authors: Prilipov
authors: Konstantin S
authors: KS
authors: Kochergin-Nikitsky
authors: Alla A
authors: AA
authors: Kushch
authors: Olga V
authors: OV
authors: Masalova
authors: Regina R
authors: RR
authors: Klimova
authors: Asya K
authors: AK
authors: Gitelman
authors: Nikolai V
authors: NV
authors: Kaverin
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology*; D004372:Ducks; D000939:Epitopes / Q000235:genetics / Q000276:immunology*; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000276:immunology* / Q000302:isolation & purification; D005585:Influenza in Birds / Q000821:virology; D007251:Influenza, Human / Q000821:virology; D050505:Mutant Proteins / Q000235:genetics / Q000276:immunology; D019590:Nucleocapsid Proteins; D011485:Protein Binding; D016601:RNA-Binding Proteins / Q000276:immunology*; D014758:Viral Core Proteins / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000939:Epitopes; D050505:Mutant Proteins; C048842:NP protein, Influenza A virus; D019590:Nucleocapsid Proteins; D016601:RNA-Binding Proteins; D014758:Viral Core Proteins