title: In silico identification of microRNAs predicted to regulate the drug metabolizing cytochrome P450 genes.
abstract: OBJECTIVE
  Cytochrome P450 (CYP) enzymes exhibit high interindividual variability that is not completely explained by known environmental and genetic factors. To further understand this variability, we hypothesized that microRNAs (miRNAs) may regulate CYP expression.
  
  
  METHODS
  MiRNA identification algorithms were used to identify the miRNAs that are predicted to regulate twelve major drug metabolizing CYPs and to identify polymorphisms in CYP mRNA 3'-UTRs that are predicted to interfere with normal mRNA-miRNA interactions.
  
  
  RESULTS
  All twelve CYPs were predicted to be targets of miRNAs. Additionally, 38 SNPs in CYP mRNA 3'-UTRs were predicted to interfere with miRNA targeting of mRNAs. These predicted miRNAs and SNPs are candidates for future in vitro studies focused on understanding the molecular regulation of these CYP genes.
  
  
  CONCLUSIONS
  These in silico results provide strong support for a role of miRNA in the regulation and variability of CYP expression.
authors: Indiana University School of Medicine, Department of Medicine, Division of Clinical Pharmacology, Indianapolis, IN, USA.
authors: Anuradha
authors: A
authors: Ramamoorthy
authors: Todd C
authors: TC
authors: Skaar
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D019295:Computational Biology; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D005786:Gene Expression Regulation; D006801:Humans; D035683:MicroRNAs / Q000502:physiology*; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D020641:Polymorphism, Single Nucleotide
chemical_list: D020413:3' Untranslated Regions; D035683:MicroRNAs; D004364:Pharmaceutical Preparations; D003577:Cytochrome P-450 Enzyme System