title: Generation of knockout mice expressing a GFP-reporter under the control of the Lmx1a locus.
abstract: Lmx1a is a member of the LIM homeodomain containing transcription factors and plays an important role during embryonic development. Specifically, it is required for the proper formation of several structures in the central nervous system, such as the roof plate, the cerebellum, and the inner ear. All these defects may contribute to the neurological phenotype observed in dreher mice, lacking functional Lmx1a protein. Interestingly, this factor was also found to promote midbrain dopaminergic neuron fate. We have introduced Green fluorescent protein (GFP) coding sequences into the Lmx1a locus by homologous recombination, and created knockout mice where GFP recapitulates the Lmx1a endogenous expression pattern.
authors: Max Planck Institute for Biophysical Chemistry, Department of Molecular Cell Biology, Am Fassberg 11, 37077 Göttingen, Germany.
authors: Gundula
authors: G
authors: Griesel
authors: Christian
authors: C
authors: Krug
authors: Larisa
authors: L
authors: Yurlova
authors: Mihaela
authors: M
authors: Diaconu
authors: Ahmed
authors: A
authors: Mansouri
mesh_terms: D000818:Animals; D004622:Embryo, Mammalian / Q000378:metabolism; D005260:Female; D017930:Genes, Reporter / Q000235:genetics*; D056426:Genetic Loci; D049452:Green Fluorescent Proteins / Q000235:genetics* / Q000378:metabolism; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism; D060850:LIM-Homeodomain Proteins; D051379:Mice; D018345:Mice, Knockout; D009474:Neurons / Q000378:metabolism; D014157:Transcription Factors
chemical_list: D018398:Homeodomain Proteins; D060850:LIM-Homeodomain Proteins; C489692:Lmx1a protein, mouse; D014157:Transcription Factors; D049452:Green Fluorescent Proteins