title: Effect of straw size and thawing time on quality of cryopreserved buffalo (Bubalus bubalis) semen.
abstract: This study was designed to compare the effect of straw size (0.25 vs. 0.5 ml) and thawing time (30 vs. 60 sec) on the quality of cryopreserved buffalo bull semen. Sperm motility, plasma membrane integrity and viability were higher (p ≤ 0.05) in 0.25 ml than 0.5 ml straw, thawed at 37°C either for 30 or 60 sec. In conclusion, cryopreservation of buffalo semen in 0.25 ml straw resulted in a higher post-thaw quality.
authors: Animal Physiology Laboratory, Department of Zoology, Pir Mehr Ali Shah Arid Agriculture University Rwalpindi, Pakistan.
authors: Muhammad S
authors: MS
authors: Ansari
authors: Bushra A
authors: BA
authors: Rakha
authors: Syed M H
authors: SM
authors: Andrabi
authors: Shamim
authors: S
authors: Akhter
mesh_terms: D000818:Animals; D002020:Buffaloes / Q000502:physiology*; D015925:Cryopreservation / Q000662:veterinary*; D007315:Insemination, Artificial / Q000662:veterinary; D008297:Male; D012661:Semen / Q000502:physiology*; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013081:Sperm Motility / Q000502:physiology; D013094:Spermatozoa / Q000502:physiology; D013696:Temperature; D013997:Time Factors