title: IgM promotes the clearance of small particles and apoptotic microparticles by macrophages.
abstract: BACKGROUND
  Antibodies are often involved in enhancing particle clearance by macrophages. Although the mechanisms of antibody-dependent phagocytosis have been studied for IgG in greater detail, very little is known about IgM-mediated clearance. It has been generally considered that IgM does not support phagocytosis. Recent studies indicate that natural IgM is important to clear microbes and other bioparticles, and that shape is critical to particle uptake by macrophages; however, the relevance of IgM and particle size in their clearance remains unclear. Here we show that IgM has a size-dependent effect on clearance.
  
  
  RESULTS
  We used antibody-opsonized sheep red blood cells, different size beads and apoptotic cells to determine the effect of human and mouse IgM on phagocytosis by mouse alveolar macrophages. Our microscopy (light, epifluorescence, confocal) and flow cytometry data show that IgM greatly enhances the clearance of small particles (about 1-2 micron) by these macrophages. There is an inverse relationship between IgM-mediated clearance by macrophages and the particle size; however, macrophages bind and internalize many different size particles coated with IgG. We also show that IgM avidly binds to small size late apoptotic cells or bodies (2-5 micron) and apoptotic microparticles (<2 µm) released from dying cells. IgM also promotes the binding and uptake of microparticle-coated beads.
  
  
  CONCLUSIONS
  Therefore, while the shape of the particles is important for non-opsonized particle uptake, the particle size matters for antibody-mediated clearance by macrophages. IgM particularly promotes the clearance of small size particles. This finding may have wider implications in IgM-mediated clearing of antigens, microbial pathogens and dying cells by the host.
authors: Lung Innate Immunity Research Laboratory, The Hospital for Sick Children, Toronto, Ontario, Canada.
authors: Michael L
authors: ML
authors: Litvack
authors: Martin
authors: M
authors: Post
authors: Nades
authors: N
authors: Palaniyar
mesh_terms: D000818:Animals; D017209:Apoptosis; D055252:Cell-Derived Microparticles / Q000378:metabolism*; D004705:Endocytosis; D004912:Erythrocytes / Q000166:cytology / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D007075:Immunoglobulin M / Q000378:metabolism*; D016676:Macrophages, Alveolar / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D018613:Microscopy, Confocal; D008863:Microspheres; D011485:Protein Binding; D012756:Sheep
chemical_list: D007074:Immunoglobulin G; D007075:Immunoglobulin M