title: Inhibitory effect of Pleurotus eryngii extracts on the activities of allergic mediators in antigen-stimulated mast cells.
abstract: Pleurotus eryngii is an edible mushroom native to Europe, the Middle East, and North Africa, and is also grown in parts of Asia. The present study investigated the anti-allergy potential of P. eryngii extract (PEE) in antigen-stimulated RBL-2H3 mast cells. PEE inhibited allergy markers, including release of hexosaminidase and histamine, in antigen-sensitized RBL-2H3 cells. PEE also suppressed the expression and production of interleukin-4 and reduced antigen-induced NFAT and NF-κB transcriptional activity in antigen-sensitized mast cells. Moreover, PEE decreased the levels of proinflammatory cytokines and COX-2 and iNOS expression in antigen-sensitized mast cells. Finally, PEE suppressed antigen-induced signal protein phosphorylation of Lyn, PLCγ2, PKC, Akt, and MAP kinases. Taken together, these results suggest that P. eryngii extract may provide insight into the prevention and treatment of allergic and inflammatory diseases.
authors: Department of Toxicology, College of Pharmacy, Chungnam National University, Daejeon 305-764, South Korea.
authors: Eun Hee
authors: EH
authors: Han
authors: Yong Pil
authors: YP
authors: Hwang
authors: Hyung Gyun
authors: HG
authors: Kim
authors: Jae Ho
authors: JH
authors: Choi
authors: Ji Hye
authors: JH
authors: Im
authors: Ji Hye
authors: JH
authors: Yang
authors: Hyun-Uk
authors: HU
authors: Lee
authors: Sung-Sik
authors: SS
authors: Chun
authors: Young Chul
authors: YC
authors: Chung
authors: Hye Gwang
authors: HG
authors: Jeong
mesh_terms: D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D006596:Hexosaminidases / Q000378:metabolism; D006636:Histamine Release / Q000187:drug effects; D006967:Hypersensitivity / Q000188:drug therapy*; D007073:Immunoglobulin E / Q000276:immunology; D007723:Korea; D008407:Mast Cells / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D008519:Medicine, Traditional; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D050778:NFATC Transcription Factors / Q000235:genetics / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry; D020076:Pleurotus / Q000737:chemistry*
chemical_list: D018926:Anti-Allergic Agents; D016207:Cytokines; D016328:NF-kappa B; D050778:NFATC Transcription Factors; D010936:Plant Extracts; D007073:Immunoglobulin E; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat; D051546:Cyclooxygenase 2; C489691:Ptgs2 protein, rat; D006596:Hexosaminidases