title: RNA binding properties of conserved protein subunits of human RNase P.
abstract: Human nuclear RNase P is required for transcription and processing of tRNA. This catalytic RNP has an H1 RNA moiety associated with ten distinct protein subunits. Five (Rpp20, Rpp21, Rpp25, Rpp29 and Pop5) out of eight of these protein subunits, prepared in refolded recombinant forms, bind to H1 RNA in vitro. Rpp20 and Rpp25 bind jointly to H1 RNA, even though each protein can interact independently with this transcript. Nuclease footprinting analysis reveals that Rpp20 and Rpp25 recognize overlapping regions in the P2 and P3 domains of H1 RNA. Rpp21 and Rpp29, which are sufficient for reconstitution of the endonucleolytic activity, bind to separate regions in the catalytic domain of H1 RNA. Common themes and discrepancies in the RNA-protein interactions between human nuclear RNase P and its related yeast and archaeal counterparts provide a rationale for the assembly of the fully active form of this enzyme.
authors: Department of Microbiology and Molecular Genetics, IMRIC, The Hebrew University-Hadassah Medical School, Jerusalem 91120, Israel.
authors: Robert
authors: R
authors: Reiner
authors: Noa
authors: N
authors: Alfiya-Mor
authors: Mishka
authors: M
authors: Berrebi-Demma
authors: Donna
authors: D
authors: Wesolowski
authors: Sidney
authors: S
authors: Altman
authors: Nayef
authors: N
authors: Jarrous
mesh_terms: D001324:Autoantigens / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites; D006801:Humans; D008274:Magnesium / Q000494:pharmacology; D008969:Molecular Sequence Data; D017510:Protein Folding; D021122:Protein Subunits / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012313:RNA / Q000737:chemistry / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D043262:Ribonuclease P / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012260:Ribonucleases / Q000235:genetics / Q000378:metabolism; D012261:Ribonucleoproteins / Q000235:genetics / Q000378:metabolism
chemical_list: D001324:Autoantigens; C112706:POP7 protein, human; D021122:Protein Subunits; C455399:RPP25 protein, human; D011994:Recombinant Proteins; D012261:Ribonucleoproteins; D012313:RNA; D012260:Ribonucleases; C105253:POP4 protein, human; D043262:Ribonuclease P; D008274:Magnesium