title: High-fat diet based on dried bovine brain: an effective animal model of dyslipidemia and insulin resistance.
abstract: Currently, there are no reports in the literature demonstrating any animal model that ingests one of the fattiest animal food source, the bovine brain. We hypothesized that a high-fat diet (HFD), based on dried bovine brain, could be used to develop an animal model possessing a spectrum of insulin resistance-related features. The HFD was formulated with 40% dried bovine brain plus 16.4% butter fat, prepared in-house. Furthermore, the diet contained 52% calories as fat and 73% of total fatty acids were saturated. Swiss mice weighing about 40 g were assigned to two dietary groups (n=6/group), one group received a standard chow diet and the other was given HFD for 3 months. The body weight and biochemical parameters of the animals were measured initially and at monthly intervals until the end of the experiment. Animals fed on a HFD showed a significant increase in the body and adipose tissue weight, serum total cholesterol and triglyceride levels, when compared with mice fed on the control diet. Additionally, the HFD group showed higher circulating levels of liver transaminases, such as alanine aminotransferase and aspartate aminotransferase, compared with the control group. Finally, to illustrate the usefulness of this model, we report that the HFD induced mild hyperglycemia, fasting hyperinsulinemia, and increased the homeostasis model of assessment (HOMA-IR), in comparison with the control group. In conclusion, our results show that HFD, based on dried bovine brain, causes insulin resistance-related metabolic disturbances. Thus, this may be a suitable model to study disturbances in energy metabolism and their consequences.
authors: Departamento de Bioquímica-Centro de Ciências Biológicas, Universidade Federal de Pernambuco, Av. Prof. Moraes Rego s/n, Cidade Universitária, Recife, PE, Brazil.
authors: Tiago Gomes
authors: TG
authors: Araújo
authors: Ana Catarina Rezende
authors: AC
authors: Leite
authors: Caíque Silveira
authors: CS
authors: Martins da Fonseca
authors: Bruno Melo
authors: BM
authors: Carvalho
authors: Alexandre Ricardo Pereira
authors: AR
authors: Schuler
authors: Vera Lúcia de Menezes
authors: VL
authors: Lima
mesh_terms: D000273:Adipose Tissue / Q000473:pathology; D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight; D001921:Brain; D002417:Cattle; D003890:Desiccation; D004041:Dietary Fats / Q000008:administration & dosage*; D004195:Disease Models, Animal; D050171:Dyslipidemias / Q000209:etiology*; D002149:Energy Intake; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance; D008055:Lipids / Q000097:blood; D008297:Male; D051379:Mice; D009929:Organ Size
chemical_list: D001786:Blood Glucose; D004041:Dietary Fats; D007328:Insulin; D008055:Lipids; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase