title: Fabrication of nano-hydroxyapatite on electrospun silk fibroin nanofiber and their effects in osteoblastic behavior.
abstract: In this study, a novel tissue engineering scaffold material of electrospun silk fibroin/nano-hydroxyapatite (nHA) biocomposite was prepared by means of an effective calcium and phosphate (Ca-P) alternate soaking method. nHA was successfully produced on regenerated silk fibroin nanofiber as a substrate within several minutes without any pretreatments. The morphologies of both nonmineralized and mineralized nanofibers were analyzed using a field-emission scanning electron microscopy (FESEM). The crystallographic phases of the nHA were analyzed using X-ray diffraction (XRD). Fourier transform infrared (FTIR) spectrophotometer and thermogravimetry analyses (TGA) were employed to determine the type of functional groups and the amount of nHA presenting in the silk/nHA biocomposite nanofibers, respectively. The osteoblastic activities of this novel nanofibrous biocomposite scaffold were also investigated by employing osteoblastic-like MC3T3-E1 cell line. The cell functionality such as alkaline phosphatase (ALP) activity was ameliorated on mineralized nanofibers. All these results indicated that this silk/nHA biocomposite scaffold material may be a promising biomaterial for bone tissue engineering.
authors: Interdisciplinary Division of Science and Technology, Graduate School, Shinshu University, Ueda 386-8567, Japan.
authors: Kai
authors: K
authors: Wei
authors: Yuan
authors: Y
authors: Li
authors: Kyu-Oh
authors: KO
authors: Kim
authors: Yuya
authors: Y
authors: Nakagawa
authors: Byoung-Suhk
authors: BS
authors: Kim
authors: Koji
authors: K
authors: Abe
authors: Guo-Qiang
authors: GQ
authors: Chen
authors: Ick-Soo
authors: IS
authors: Kim
mesh_terms: D016475:3T3 Cells; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D012831:Bombyx / Q000737:chemistry*; D002448:Cell Adhesion; D049109:Cell Proliferation; D017886:Durapatite / Q000737:chemistry* / Q000378:metabolism; D005349:Fibroins / Q000737:chemistry* / Q000378:metabolism; D051379:Mice; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D005349:Fibroins; D017886:Durapatite; D000469:Alkaline Phosphatase