title: Light stress photodynamics of chlorophyll-binding proteins in Arabidopsis thaliana thylakoid membranes revealed by high-resolution mass spectrometric studies.
abstract: In higher plants the light energy is captured by the photosynthetic pigments that are bound to photosystem I and II and their light-harvesting complex (LHC) subunits. In this study, we examined the photodynamic changes within chlorophyll-protein complexes in the thylakoid membrane of Arabidopsis thaliana leaves adapted to low light and subsequently exposed to light stress. Chlorophyll-protein complexes were isolated using sucrose density gradient centrifugation and blue-native polyacrylamid gel electrophoresis (BN-PAGE). Proteome analysis was performed using SDS-PAGE, HPLC and high resolution mass spectrometry. We identified several rarely expressed and stress-induced chlorophyll-binding proteins, showed changes in localization of early light-induced protein family and LHC protein family members between different photosynthetic complexes and assembled/disassembled subcomplexes under light stress conditions and discuss their role in a variety of light stress-related processes.
authors: Institute of Biochemical Physics, Russian Academy of Sciences, 119334 Moscow, Russia.
authors: D N
authors: DN
authors: Galetskiy
authors: J N
authors: JN
authors: Lohscheider
authors: A S
authors: AS
authors: Kononikhin
authors: O N
authors: ON
authors: Kharybin
authors: I A
authors: IA
authors: Popov
authors: I
authors: I
authors: Adamska
authors: E N
authors: EN
authors: Nikolaev
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000378:metabolism / Q000528:radiation effects*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D008027:Light; D045342:Light-Harvesting Protein Complexes / Q000378:metabolism*; D008969:Molecular Sequence Data; D045331:Photosystem I Protein Complex / Q000378:metabolism; D045332:Photosystem II Protein Complex / Q000378:metabolism; D020543:Proteome / Q000378:metabolism; D013312:Stress, Physiological; D053719:Tandem Mass Spectrometry; D020524:Thylakoids / Q000378:metabolism / Q000528:radiation effects*
chemical_list: D029681:Arabidopsis Proteins; D045342:Light-Harvesting Protein Complexes; D045331:Photosystem I Protein Complex; D045332:Photosystem II Protein Complex; D020543:Proteome