title: In vitro metabolism of trans-permethrin and its major metabolites, PBalc and PBacid, in humans.
abstract: To estimate the metabolic profile of trans-permethrin in humans, a comparison of the in vitro metabolism of trans-permethrin in humans and rats was conducted using hepatic microsomes, and cytochrome P450 and UDP-glucuronyltransferase isoforms, which catalyze the metabolism of 3-phenoxybenzyl alcohol (PBalc) and 3-phenoxybenzoic acid (PBacid), respectively. In humans and rats, the major metabolic reaction of trans-permethrin in microsomal incubations was the cleavage of ester linkage to give PBalc, followed by oxidation to 4'-OH-PBalc, 4'-OH-PBacid, and PBacid. As to 4'-hydroxylation of PBalc, several CYPs were able to catalyze the reaction, and CYP2E1 was identified as a predominant isoform. PBacid and its conjugates (glucuronide and glycine) are major urinary metabolites of trans-permethrin in mammals. PBacid is also a metabolite of several pyrethroids, and has been used as a biomarker of human exposure to pyrethroids. Our study indicated that there was no difference in glucuronyltransferase activity of PBacid between humans and rats, and that only UGT1A9 can catalyze the glucuronidation of PBacid among human UGTs. Some UGT1A9 variants are known to have poor glucuronidation activity. From these results, it was assumed that deficiency or polymorphism of UGT1A9 might affect the profile of PBacid and its conjugates in urine collected from persons exposed to trans-permethrin or other pyrethroids. These results are helpful for understanding the metabolism of trans-permethrin in humans and determining methods for quantification of target analytes for assessment of human exposure to trans-permethrin and other pyrethroids that give PBacid and its conjugates as urinary metabolites.
authors: Environmental Health Science Laboratory, Sumitomo Chemical, Co., Ltd. 1-98, 3-Chome, Kasugade-naka Konohana-ku, Osaka, Japan. takakut@sc.sumitomo-chem.co.jp
authors: Tomoyuki
authors: T
authors: Takaku
authors: Kazuki
authors: K
authors: Mikata
authors: Masayoshi
authors: M
authors: Matsui
authors: Kazuhiko
authors: K
authors: Nishioka
authors: Naohiko
authors: N
authors: Isobe
authors: Hideo
authors: H
authors: Kaneko
mesh_terms: D000818:Animals; D001565:Benzoates / Q000378:metabolism*; D001592:Benzyl Alcohols / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology / Q000378:metabolism; D023421:Models, Animal; D026023:Permethrin / Q000378:metabolism*; D010575:Pesticides / Q000737:chemistry / Q000378:metabolism*; D051381:Rats
chemical_list: D001565:Benzoates; D001592:Benzyl Alcohols; D010575:Pesticides; C064809:3-phenoxybenzylalcohol; D026023:Permethrin; C017618:3-phenoxybenzoic acid; D003577:Cytochrome P-450 Enzyme System