title: Expression of connective tissue growth factor (CTGF/CCN2) in breast cancer cells is associated with increased migration and angiogenesis.
abstract: Connective tissue growth factor (CTGF/CCN2) belongs to the CCN family of matricellular proteins, comprising Cyr61, CTGF, NovH and WISP1-3. The CCN proteins contain an N-terminal signal peptide followed by four conserved domains sharing sequence similarities with the insulin-like growth factor binding proteins, von Willebrand factor type C repeat, thrombospondin type 1 repeat, and a C-terminal growth factor cysteine knot domain. To investigate the role of CCN2 in breast cancer, we transfected MCF-7 cells with full-length CCN2, and with four mutant constructs in which one of the domains had been deleted. MCF-7 cells stably expressing full-length CCN2 demonstrated reduced cell proliferation, increased migration in Boyden chamber assays and promoted angiogenesis in chorioallantoic membrane assays compared to control cells. Deletion of the C-terminal cysteine knot domain, but not of any other domain-deleted mutants, abolished activities mediated by full-length CCN2. We have dissected the role of CCN2 in breast tumorigenesis on a structural basis.
authors: Department of Hematology and Oncology, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
authors: Wenwen
authors: W
authors: Chien
authors: James
authors: J
authors: O'Kelly
authors: Daning
authors: D
authors: Lu
authors: Amanda
authors: A
authors: Leiter
authors: Julia
authors: J
authors: Sohn
authors: Dong
authors: D
authors: Yin
authors: Beth
authors: B
authors: Karlan
authors: Jay
authors: J
authors: Vadgama
authors: Karen M
authors: KM
authors: Lyons
authors: H Phillip
authors: HP
authors: Koeffler
mesh_terms: D017209:Apoptosis / Q000235:genetics; D001943:Breast Neoplasms; D002448:Cell Adhesion / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D055513:Connective Tissue Growth Factor / Q000235:genetics* / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009154:Mutation / Q000235:genetics; D009389:Neovascularization, Pathologic / Q000235:genetics / Q000378:metabolism / Q000473:pathology
chemical_list: D055513:Connective Tissue Growth Factor