title: Visualization of induced RNA in single bacterial cells.
abstract: Visualization of RNA in live cells is a challenging task due to the transient character of most RNA molecules and the lack of adequate methods to label RNA noninvasively. Here, we describe a system for regulated RNA synthesis and visualization of RNA in live Escherichia coli cells based on protein complementation. This method allows for labeling RNA with a relatively small protein complex that becomes fluorescent only when bound to an RNA. This method greatly reduces the high fluorescence background characteristic of methods employing intact fluorescent proteins. A short reporter RNA was shown to localize at the cell periphery in nonrandom patterns.
authors: Department of Biomedical Engineering, Center for Advanced Biotechnology, College of Engineering, Boston University, Boston, MA, USA.
authors: Azra
authors: A
authors: Borogovac
authors: Natalia E
authors: NE
authors: Broude
mesh_terms: D052157:Aptamers, Nucleotide / Q000378:metabolism; D018914:Cells, Immobilized / Q000166:cytology / Q000378:metabolism; D004926:Escherichia coli / Q000166:cytology* / Q000235:genetics* / Q000378:metabolism; D005434:Flow Cytometry; D017930:Genes, Reporter / Q000235:genetics; D005898:Glass / Q000737:chemistry; D007700:Kinetics; D008856:Microscopy, Fluorescence; D057054:Molecular Imaging / Q000379:methods*; D010957:Plasmids / Q000235:genetics; D014176:Protein Biosynthesis; D012329:RNA, Bacterial / Q000032:analysis* / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D059010:Single-Cell Analysis / Q000379:methods*; D013499:Surface Properties; D014158:Transcription, Genetic
chemical_list: D052157:Aptamers, Nucleotide; D012329:RNA, Bacterial