title: Fast determination of oleic acid in pork by flow injection analysis/mass spectrometry.
abstract: In some Mediterranean products such as olive oil or ham, oleic acid is the most abundant component of the total fat. Due to the large volume of trade in these products, it may be necessary to analyze oleic fatty acids in high numbers of samples in short periods of time. However, using classic lipid analysis techniques, it is not always possible to cope with these high demands. To solve this problem, a high-throughput analytical method for oleic fatty acid quantification in pork is presented. The purpose of the method is to avoid liquid chromatography processes using a flow injection analysis (FIA) system based on electrospray ionization mass spectrometry. The use of pentadecanoic fatty acid as an internal standard overcame matrix effects. The oleic FIA technique could be used as a suitable method for discriminating carcass samples for selection and labeling by oleic acid content when large numbers of pork samples must be processed in a short period of time.
authors: Departament de Producció Animal, Universitat de Lleida, Rovira Roure 191, 25198, Lleida, Spain.
authors: Rebeca
authors: R
authors: Muñoz
authors: Francisca
authors: F
authors: Vilaró
authors: Jordi
authors: J
authors: Eras
authors: Joan
authors: J
authors: Estany
authors: Marc
authors: M
authors: Tor
mesh_terms: D000818:Animals; D002849:Chromatography, Gas; D005227:Fatty Acids; D017022:Flow Injection Analysis / Q000379:methods*; D057166:High-Throughput Screening Assays / Q000379:methods*; D016014:Linear Models; D008460:Meat / Q000032:analysis*; D019301:Oleic Acid / Q000032:analysis*; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013552:Swine; D013997:Time Factors
chemical_list: D005227:Fatty Acids; D019301:Oleic Acid; C117025:pentadecanoic acid