title: Addition of zinc methacrylate in dental polymers: MMP-2 inhibition and ultimate tensile strength evaluation.
abstract: This study evaluated the effect of zinc methacrylate (ZM) on the inhibition of matrix metalloproteinase 2 (MMP-2) and the ultimate tensile strength (UTS) of an experimental polymer. Enzymes secreted from mouse gingival tissues were analyzed by gelatin zymography in buffers containing 5 mM CaCl(2) (Tris-CaCl(2)) in 50 mM Tris-HCl buffer with various concentrations of ZM (0.5, 1, 2, 4, 8, and 16 mM). The matrix metalloproteinases present in the conditioned media were characterized by immunoprecipitation. The polymer UTS evaluation was performed in eight groups with various concentrations of ZM (0, 0.5, 1, 2.5, 5, 10, 20, and 30 wt.%), in a mechanical testing machine. MMP-2 (62 kDa) was detected in the zymographic assays and inhibited by ZM in all tested concentrations. UTS data were submitted to one-way ANOVA and Tukey's test (α = 0.05), and no significant differences were observed among groups, except in the polymer containing 30% ZM, presenting a significantly lower value when compared with the control group (p < 0.05). The results suggest that ZM inhibits MMP-2 expression in all concentrations tested, while small concentrations did not affect the ultimate tensile strength of the polymer. Zinc methacrylate is a metalloproteinase inhibitor that can be copolymerized with other methacrylate monomers. Yet, the addition of ZM did not affect the resin bond strength. Thus, in vivo tests should be performed to evaluate the performance of this material.
authors: Operative Dentistry Department, School of Dentistry, Federal University of Pelotas, Rua Gonçalves Chaves, 457, Pelotas, RS 96015-560, Brazil.
authors: Sandrina
authors: S
authors: Henn
authors: Rodrigo Varella
authors: RV
authors: de Carvalho
authors: Fabrício Aulo
authors: FA
authors: Ogliari
authors: Ana Paula
authors: AP
authors: de Souza
authors: Sergio Roberto Peres
authors: SR
authors: Line
authors: Adriana Fernandes
authors: AF
authors: da Silva
authors: Flávio Fernando
authors: FF
authors: Demarco
authors: Adriana
authors: A
authors: Etges
authors: Evandro
authors: E
authors: Piva
mesh_terms: D000818:Animals; D017077:Culture Media, Conditioned; D003764:Dental Materials / Q000737:chemistry*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005881:Gingiva / Q000201:enzymology; D008422:Materials Testing; D020778:Matrix Metalloproteinase 2; D061965:Matrix Metalloproteinase Inhibitors; D008689:Methacrylates / Q000737:chemistry* / Q000494:pharmacology; D051379:Mice; D011092:Polyethylene Glycols / Q000737:chemistry*; D011109:Polymethacrylic Acids / Q000737:chemistry*; D013314:Stress, Mechanical; D013718:Tensile Strength; D046509:Tissue Culture Techniques; D017967:Zinc Compounds / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D017077:Culture Media, Conditioned; D003764:Dental Materials; D004791:Enzyme Inhibitors; D061965:Matrix Metalloproteinase Inhibitors; D008689:Methacrylates; D011109:Polymethacrylic Acids; D017967:Zinc Compounds; C020946:triethylene glycol dimethacrylate; D011092:Polyethylene Glycols; D020778:Matrix Metalloproteinase 2; C522362:Mmp2 protein, mouse