title: Simultaneous determination of 5'-monophosphate nucleotides in infant formulas by HPLC-MS.
abstract: A method was developed for simultaneous determination of 5'-monophosphate nucleotides, adenosine 5'-monophosphate, cytidine 5'-monophosphate, guanosine 5'-monophosphate, inosine 5'-monophosphate, and uridine 5'-monophosphate in infant formulas by high-performance liquid chromatography-mass spectrometry equipped with electrospray ionization source. The complete chromatographic separation of five nucleotides was achieved through a Symmetry C(18) column, after a binary gradient elution with water containing 0.1% formic acid and acetonitrile as mobile phase. The multi-reaction monitoring mode was applied for tandem mass spectrometry analysis. The established method was further validated by determining the linearity (R(2) > 0.999), recovery (92.0-105.0%), and precision (relative standard deviation ≤6.97%). To verify the applicability of the method, thirty commercially available infant formulas were randomly purchased from the supermarkets in Hangzhou, China, and then analyzed. The results showed that the developed method is validated, sensitive, and reliable for quantitation of nucleotides in infant formulas.
authors: Zhejiang Provincial Center for Disease Prevention and Control, Hangzhou, Zhejiang 310051, PR China.
authors: Yiping
authors: Y
authors: Ren
authors: Jingshun
authors: J
authors: Zhang
authors: Xiaodan
authors: X
authors: Song
authors: Xiaochun
authors: X
authors: Chen
authors: Duo
authors: D
authors: Li
mesh_terms: D000097:Acetonitriles; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D007223:Infant; D041943:Infant Formula / Q000737:chemistry*; D016014:Linear Models; D011685:Purine Nucleotides / Q000032:analysis*; D011742:Pyrimidine Nucleotides / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000097:Acetonitriles; D011685:Purine Nucleotides; D011742:Pyrimidine Nucleotides; C032159:acetonitrile