title: Determining DNA methylation profiles using sequencing.
abstract: Cytosine methylation is an epigenetic mark that has a significant impact on the regulation of transcription and replication of DNA. DNA methylation patterns are highly conserved across cell divisions and are therefore highly heritable. Furthermore, in multicellular organisms, DNA methylation patterning is a key determinant of cellular differentiation and tissue-specific expression patterns. Lastly, DNA demethylases can affect global levels of DNA methylation during specific stages of development. Bisulfite sequencing is considered the gold standard for measuring the methylation state of cytosines. Sodium bisulfite -converts unmethylated cytosines to uracils (which after PCR are converted to thymines), while leaving methylated cytosines unconverted. By mapping bisulfite treated DNA back to the original reference genome, it is then possible to determine the methylation state of individual cytosines. With the advent of next-generation sequencers during the past few years, it is now possible to determine the methylation state of an entire genome. Here, we describe in detail two protocols for preparing bisulfite treated libraries, which may be sequenced using Illumina GAII sequencers. The first of these uses premethylated adapters, which are not affected by bisulfite treatments, while the second uses a two-stage adapter strategy and does not require premethylation of the adapters. We also describe the specialized protocol for mapping bisulfite converted reads. These approaches allow one to determine the methylation state of each cytosine in the genome.
authors: Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA, USA.
authors: Suhua
authors: S
authors: Feng
authors: Liudmilla
authors: L
authors: Rubbi
authors: Steven E
authors: SE
authors: Jacobsen
authors: Matteo
authors: M
authors: Pellegrini
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000235:genetics* / Q000378:metabolism*; D019175:DNA Methylation; D004260:DNA Repair; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D015723:Gene Library; D026723:Polyadenylation; D016133:Polymerase Chain Reaction; D011786:Quality Control; D017422:Sequence Analysis, DNA / Q000379:methods*; D012984:Software; D013447:Sulfites / Q000378:metabolism
chemical_list: D013447:Sulfites; D004247:DNA; C020102:endodeoxyribonuclease DpnI; D015252:Deoxyribonucleases, Type II Site-Specific; C042345:hydrogen sulfite