title: A further note on the sampling device for the anti-mutagenicity of saliva.
abstract: OBJECTIVE
  The purpose of this study was to compare the anti-mutagenicity of Salivette and test-tube sampling saliva. In addition, the relation between the inhibiting and pH-buffering capacities of saliva was investigated.
  
  
  METHODS
  Subjects were 52 healthy female university students. The collection of saliva samples was carried out using 2 sampling devices; test-tube and Salivette. The anti-mutegenicity of the saliva was measured using the umu test.
  
  
  RESULTS
  The inhibiting capacity of Salivette-saliva was significantly lower compared with that of testube-saliva (p<0.01,t test). However, there was a significant correlation between them (r=0.35; p<0.05). In addition, there was a significant correlation between the inhibiting and pH-buffering capacities of saliva (r=-0.36; p<0.05).
  
  
  CONCLUSIONS
  These findings suggest that both the Salivette and the test-tube may be appropriate as saliva-sampling devices. In addition, they suggest that the bicarbonates might inhibit the anti-mutagenicity of saliva, or that the activity of substances related to the anti-mutagenicity of saliva might be dependent on pH.
authors: Department of Social and Environmental Medicine, Osaka University Graduate School of Medicine, 2-2 Yamada-Oka, 565-0871, Sutta, Osaka, Japan.
authors: Masahiro
authors: M
authors: Toda
authors: Kanehisa
authors: K
authors: Morimoto
authors: Sei-Ichi
authors: S
authors: Nakamura
authors: Kazuo
authors: K
authors: Hayakawa