title: Ring-opening metathesis polymerization-derived monolithic strong anion exchangers for the separation of 5'-phosphorylated oligodeoxythymidylic acids fragments.
abstract: Ring-opening metathesis polymerization (ROMP) derived monoliths were prepared from 5-norborn-2-enemethyl bromide (NBE-CH(2)Br) and tris(5-norborn-2-enemethoxy)methylsilane ((NBE-CH(2)O)(3)SiCH(3)) within the confines of surface-silanized borosilicate columns (100 mm × 3 mm I.D.), applying Grubbs' first generation benzylidene-type catalyst [RuCl(2)(PCy(3))(2)(CHPh)]. Two monoliths of the same recipe were converted into strong anion-exchangers applying two different approaches. Monolith I was prepared by a two-step reaction of the poly(NBE-CH(2)-Br) moieties with diethyl amine forming a weak-anion exchanger followed by reaction (quaternization) with ethyl iodide. Monolith II was prepared via a single-step reaction of the poly(NBE-CH(2)-Br) moieties with triethyl amine. The resulting monolithic anion-exchangers prepared demonstrated a good aptitude for the anion-exchange separation of single-stranded nucleic acids (ss-DNA). However, monolith II showed superior separation efficiency compared to monolith I indicated by sharper analyte peaks and better resolution values for the 5'-phosphorylated oligodeoxythymidylic acids fragments. On monolith II, the seven fragments of [d(pT)(12-18)] were baseline separated in less than 9 min. The influence of the buffer pH on the separation efficiency was studied applying a phosphate (0.05 mol/L, pH 7 and 8) and Tris-HCl buffer (0.05 mol/L, pH 9), respectively.
authors: Lehrstuhl für Makromolekulare Stoffe und Faserchemie, Institut für Polymerchemie, Universität Stuttgart, D-70569 Stuttgart, Germany. said.lubbad@ipoc.uni-stuttgart.de
authors: Said H
authors: SH
authors: Lubbad
authors: Rajendar
authors: R
authors: Bandari
authors: Michael R
authors: MR
authors: Buchmeiser
mesh_terms: D000838:Anions / Q000737:chemistry; D002852:Chromatography, Ion Exchange / Q000295:instrumentation* / Q000379:methods; D006863:Hydrogen-Ion Concentration; D007475:Ion Exchange Resins / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D058105:Polymerization; D011312:Pressure; D015203:Reproducibility of Results
chemical_list: D000838:Anions; D007475:Ion Exchange Resins; D009838:Oligodeoxyribonucleotides; C027903:oligo (dT)