title: Wfs1 mutation makes mice sensitive to insulin-like effect of acute valproic acid and resistant to streptozocin.
abstract: Valproic acid (VLP) is a widely used anticonvulsant and mood-stabilizing drug that relieves the endoplasmic reticulum (ER) stress response, a pathogenetic process related to diabetes. The aim of the present study was to evaluate whether acute valproic acid is able to interfere with glucose intolerance in two different diabetes models: The first model was a Wfs1 mutant mouse with an elevated ER stress response and the second model a streptozocin-induced diabetic mouse. VLP (300 mg/kg, i.p.) was administered to Wfs1 knockout (KO) mice and glucose tolerance test was performed 15 min later. VLP did not have an effect on the course of the glucose tolerance test in wild-type mice, while it did normalize the glucose intolerance in Wfs1 knockout mice. Acute valproic acid also lowered the blood glucose levels in streptozocin-treated mice and potentiated the effect of insulin in these mice. Thus, acute valproic acid is effective in lowering blood glucose levels possibly by potentiating insulin action in both Wfs1 KO mice and in streptozocin-induced type 1 diabetic mice.
authors: Department of Physiology, University of Tartu, 19 Ravila Street, Tartu, Estonia. anton.terasmaa@ut.ee
authors: Anton
authors: A
authors: Terasmaa
authors: Ursel
authors: U
authors: Soomets
authors: Julia
authors: J
authors: Oflijan
authors: Marite
authors: M
authors: Punapart
authors: Mats
authors: M
authors: Hansen
authors: Vallo
authors: V
authors: Matto
authors: Kersti
authors: K
authors: Ehrlich
authors: Anne
authors: A
authors: Must
authors: Sulev
authors: S
authors: Kõks
authors: Eero
authors: E
authors: Vasar
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000235:genetics; D001835:Body Weight / Q000235:genetics; D003404:Creatinine / Q000652:urine; D004351:Drug Resistance / Q000235:genetics*; D005947:Glucose; D006029:Glycosuria; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D007328:Insulin / Q000097:blood; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009154:Mutation; D010179:Pancreas / Q000187:drug effects / Q000473:pathology; D013311:Streptozocin / Q000494:pharmacology*; D014635:Valproic Acid / Q000494:pharmacology*
chemical_list: D001786:Blood Glucose; D007004:Hypoglycemic Agents; D007328:Insulin; D008565:Membrane Proteins; C114987:wolframin protein; D013311:Streptozocin; D014635:Valproic Acid; D003404:Creatinine; D005947:Glucose