title: [Effect of aflatoxin G1 on the expression of HLA- I molecule in human esophageal epithelial cells].
abstract: OBJECTIVE
  To explore the effect of AFG1 on the expression of human leukocyte antigen (HLA-I) molecule in human esophageal epithelial cells.
  
  
  METHODS
  Western Blot and RT-PCT analysis was used to explore the effect of AFG1 on the antigen presenting function of primary cultured esophageal epithelial cells.
  
  
  RESULTS
  The protein expression of HLA-ABC was significantly decreased in the groups treated with 100, 1000 and 2000 microg/L AFG1 as compared with the solvent control group (P < 0.05). Within the range of 100 - 2000 microg/L AFG1, the protein expression level of HLA-ABC of esophageal epithelial cells decreased gradually, and showed significant negative correlation (r = -0.921, n = 3, P < 0.01). The effect of AFG1 on the expression of HLA-ABC mRNA was further confirmed by RT-PCR analysis. The results showed that after treated with 100 microg/L, 1000 microg/L and 2000 microg/L AFG1, the expression of HLA-A mRNA was decreased significantly as compared with the solvent control (P < 0.05), and the expression of HLA-B mRNA was decreased in the 2000 microg/L AFG1 treated group (P < 0.05), while there was no effect on the expression of HLA-C mRNA.
  
  
  CONCLUSIONS
  The expression of HLA- I molecule in human esophageal epithelial cells could be inhibited by AFG1 treatment.
authors: The First Hospital of Hebei Medical University, Shijiazhuang 050031, China. lizengning@126.com
authors: Zengning
authors: Z
authors: Li
authors: Jinfeng
authors: J
authors: Cui
authors: Xianghong
authors: X
authors: Zhang
authors: Junling
authors: J
authors: Wang
mesh_terms: D000348:Aflatoxins / Q000494:pharmacology*; D002478:Cells, Cultured; D015536:Down-Regulation; D004847:Epithelial Cells / Q000166:cytology*; D004947:Esophagus / Q000166:cytology*; D015234:HLA-A Antigens / Q000235:genetics / Q000378:metabolism; D015235:HLA-B Antigens / Q000235:genetics / Q000378:metabolism; D015236:HLA-C Antigens / Q000235:genetics / Q000378:metabolism; D015395:Histocompatibility Antigens Class I / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D000348:Aflatoxins; D015234:HLA-A Antigens; D015235:HLA-B Antigens; D015236:HLA-C Antigens; D015395:Histocompatibility Antigens Class I; D012333:RNA, Messenger; C027955:aflatoxin G1