title: Molecular characterization of arabinoxylans from hull-less barley milling fractions.
abstract: Arabinoxylans were prepared from different hull-less barley milling fractions (bran, shorts and flour). The yields of hull-less bran arabinoxylan (HBB-AX), shorts arabinoxylan (HBS-AX) and flour arabinoxylan (HBF-AX) were 8.42%, 4.08% and 2.13% respectively. Sugar composition analysis showed that arabinose and xylose were the main sugars. HBF-AX had the highest Ara/Xyl ratio, followed by HBS-AX and HBB-AX. Size exclusion chromatography analysis (HPSEC) showed that HBF-AX had the highest molecular weight, followed by HBS-AX and HBB-AX, which had the lowest molecular weight. Aqueous solutions of HBB-AX, HBS-AX and HBF-AX had higher molecular weight(Mw) than in 0.5 mol/L NaOH and 1.0 mol/L NaOH. Dynamic light scattering analysis (DLS) showed that HBB-AX, HBS-AX and HBF-AX had existed in two states in distilled water, 0.5 mol/L NaOH, 1.0 mol/L NaOH, and DMSO/H₂O (90/10), an unaggregated state and an aggregated state, with the latter predominating.
authors: Grain College, Henan University of Technology, Zhengzhou 450052, Henan Province, China. xuelingzheng@haut.edu.cn
authors: Xueling
authors: X
authors: Zheng
authors: Limin
authors: L
authors: Li
authors: Xiaoxi
authors: X
authors: Wang
mesh_terms: D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D001467:Hordeum / Q000737:chemistry*; D008027:Light; D010316:Particle Size; D012542:Scattering, Radiation; D014990:Xylans / Q000737:chemistry*
chemical_list: D014990:Xylans; C085118:arabinoxylan