title: Quantification and evaluation of infectivity of shiga toxin-encoding bacteriophages in beef and salad.
abstract: Stx bacteriophages in 68 samples of beef and salad were quantified by real-time quantitative PCR (qPCR). Stx phages from the samples were propagated in Escherichia coli C600, E. coli O157:H7, and Shigella strains and further quantified. Fifty percent of the samples carried infectious Stx phages that were isolated from plaques generated by lysis.
authors: Department of Microbiology, University of Barcelona, Diagonal 645, Annex, Floor 0, 08028 Barcelona, Spain.
authors: Lejla
authors: L
authors: Imamovic
authors: Maite
authors: M
authors: Muniesa
mesh_terms: D001435:Bacteriophages / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D004926:Escherichia coli / Q000821:virology*; D005516:Food Microbiology; D008242:Lysogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D022621:Shiga Toxin / Q000235:genetics*; D012760:Shigella / Q000821:virology*
chemical_list: D022621:Shiga Toxin