title: Fluorescence in situ hybridization methods and troubleshooting applied to fixed cell suspensions.
abstract: A robust procedure for performing fluorescence in situ hybridization (FISH) is described, with tips for troubleshooting. FISH probes are now more reliable and there is a greater range commercially available. FISH is an essential part of the cytogeneticist's repertoire. It remains a powerful, complementary adjunct to karyotype studies, and knowledge of the underlying chromosome abnormalities can be essential for understanding the FISH signal patterns.
authors: Clinical Cytogenetics Laboratory, The Royal Marsden Hospital and The Institute of Cancer Research, Sutton, Surrey, UK. john.swansbury@rmh.nhs.uk
authors: John
authors: J
authors: Swansbury
authors: Toon
authors: T
authors: Min
authors: Shilani
authors: S
authors: Aruliah
mesh_terms: D018914:Cells, Immobilized / Q000166:cytology; D015342:DNA Probes / Q000737:chemistry / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D009691:Nucleic Acid Denaturation; D013535:Suspensions
chemical_list: D015342:DNA Probes; D013535:Suspensions