title: Syntrophomonadaceae-affiliated species as active butyrate-utilizing syntrophs in paddy field soil.
abstract: DNA-based stable-isotope probing was applied to identify the active microorganisms involved in syntrophic butyrate oxidation in paddy field soil. After 14 and 21 days of incubation with [U-(13)C]butyrate, the bacterial Syntrophomonadaceae and the archaeal Methanosarcinaceae and Methanocellales incorporated substantial amounts of (13)C label into their nucleic acids. Unexpectedly, members of the Planctomycetes and Chloroflexi were also labeled with (13)C by yet-unclear mechanisms.
authors: College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, China.
authors: Pengfei
authors: P
authors: Liu
authors: Qiongfen
authors: Q
authors: Qiu
authors: Yahai
authors: Y
authors: Lu
mesh_terms: D002087:Butyrates / Q000378:metabolism*; D002247:Carbon Isotopes / Q000378:metabolism; D041862:Chloroflexi / Q000254:growth & development / Q000378:metabolism; D016000:Cluster Analysis; D019641:DNA, Archaeal / Q000737:chemistry / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D019605:Euryarchaeota / Q000254:growth & development / Q000378:metabolism; D020459:Genes, rRNA; D006094:Gram-Positive Bacteria / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism* / Q000502:physiology; D007553:Isotope Labeling; D008969:Molecular Sequence Data; D010802:Phylogeny; D019642:RNA, Archaeal / Q000235:genetics; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012988:Soil Microbiology; D013559:Symbiosis; D014529:Uridine / Q000378:metabolism
chemical_list: D002087:Butyrates; D002247:Carbon Isotopes; D019641:DNA, Archaeal; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D019642:RNA, Archaeal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D014529:Uridine