title: Integrase-defective lentiviral-vector-based vaccine: a new vector for induction of T cell immunity.
abstract: BACKGROUND
  The development of new strategies for the induction of potent and broad immune responses is of high priority in the vaccine field. In this setting, integrase-defective lentiviral vectors (IDLV) represent a new and promising delivery system for immunization purposes.
  
  
  METHODS
  In this review we describe the development and application of IDLV for vaccination. IDLV are turning out to be a new class of vectors endowed with peculiar characteristics, setting them apart from the parental integration-competent lentiviral vectors. Recent data suggest that IDLV are able to induce strong antigen-specific immune responses in terms of quantity, persistence and quality of CD8(+) T cell response following a single immunization in mice.
  
  
  CONCLUSIONS
  IDLV are a recent acquisition in the field of genetic immunization, thus allowing for the opportunity of further upgrading, including increasing antigen expression and potency of immune response. Based on recent reports showing the potential of IDLV for immunization in mouse models, further development and validation of IDLV, including comparison with other vaccine protocols and use in non-human primate models, are warranted.
authors: Department of Infectious, Parasitic and Immune-mediated Diseases, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy. donatella.negri@iss.it
authors: Donatella Rm
authors: DR
authors: Negri
authors: Zuleika
authors: Z
authors: Michelini
authors: Roberta
authors: R
authors: Bona
authors: Maria
authors: M
authors: Blasi
authors: Piero
authors: P
authors: Filati
authors: Pasqualina
authors: P
authors: Leone
authors: Alessandra
authors: A
authors: Rossi
authors: Marina
authors: M
authors: Franco
authors: Andrea
authors: A
authors: Cara
mesh_terms: D005822:Genetic Vectors; D006801:Humans; D007111:Immunity, Cellular; D019426:Integrases / Q000235:genetics*; D016086:Lentivirus / Q000235:genetics*; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D019426:Integrases