title: Amyloid-like fibrils are not detected in non-small cell lung cancer tissue samples.
abstract: Some proteins, canonically not associated with amyloid diseases, can aggregate into amyloid-like fibrils under special conditions. Our group hypothesized that stressful cancer microenvironment might induce the formation of insoluble deposits of p53 mutant protein. A cohort of 28 non-small cell lung cancer (NSCLC) patients was used to test the aforementioned hypothesis. Tumor specimens were assessed for TP53 mutations using DNA sequencing and for amyloid formation by Congo red staining. TP53 mutations were present in 57% of patients, whereas no amyloid deposits were detected in tissue sections under polarized light microscopy. Mutant p53 proteins are not associated with the appearance of amyloid-like fibrils in NSCLC samples, and DNA sequencing remains the standard method to detect such abnormality.
authors: Clinical Research Coordination, Brazilian National Cancer Institute (INCA)-CPQ, Rua André Cavalcanti, 37, 5 Andar, Rio de Janeiro 20231-050, Brazil.
authors: Luiz Henrique
authors: LH
authors: de Lima Araújo
authors: Luciana W
authors: LW
authors: Pinto
authors: Luciene F
authors: LF
authors: Schluckebier
authors: Joyce L
authors: JL
authors: de Moraes
authors: Paulo A
authors: PA
authors: Faria
authors: Emanuela
authors: E
authors: Moraes
authors: Carlos G
authors: CG
authors: Ferreira
authors: Cinthya
authors: C
authors: Sternberg
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology; D000328:Adult; D000368:Aged; D000682:Amyloid / Q000235:genetics*; D018287:Carcinoma, Large Cell / Q000235:genetics / Q000473:pathology; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000473:pathology*; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D050505:Mutant Proteins / Q000235:genetics*; D009154:Mutation / Q000235:genetics*; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction; D011379:Prognosis; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
chemical_list: D000682:Amyloid; D004273:DNA, Neoplasm; D050505:Mutant Proteins; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53