title: Downstream bioengineering of ACE chromosomes for incorporation of site-specific recombination cassettes.
abstract: Advances in mammalian artificial chromosome technology have made chromosome-based vector technology amenable to a variety of biotechnology applications including cellular protein production, genomics, and animal transgenesis. A pivotal aspect of this technology is the ability to generate artificial chromosomes de novo, transfer them to a variety of cells, and perform downstream engineering of artificial chromosomes in a tractable and rational manner. Previously, we have described an alternative artificial chromosome technology termed the ACE chromosome system, where the ACE platform chromosome contains a multitude of site-specific, recombination sites incorporated during the creation of the ACE platform chromosome. In this chapter we review a variant of the ACE chromosome technology whereby site-specific, recombination sites can be integrated into the ACE chromosome following its de novo synthesis. This variation allows insertion of user-defined, site-specific, recombination systems into an existing ACE platform chromosome. These bioengineered ACE platform chromosomes, containing user-defined recombination sites, represent an ideal circuit board to which an array of genetic factors can be plugged-in and expressed for various research and therapeutic applications.
authors: Department of Biomedical Sciences, Mercer University School of Medicine, Savannah, GA, USA.
authors: Amy L
authors: AL
authors: Greene
authors: Edward L
authors: EL
authors: Perkins
mesh_terms: D000818:Animals; D002460:Cell Line; D022221:Chromosomes, Artificial, Mammalian / Q000235:genetics*; D005434:Flow Cytometry; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D016254:Mutagenesis, Insertional / Q000379:methods*; D016297:Mutagenesis, Site-Directed / Q000379:methods*; D011995:Recombination, Genetic / Q000235:genetics*; D015203:Reproducibility of Results