title: Biosynthesis and characterization of CdS quantum dots in genetically engineered Escherichia coli.
abstract: Quantum dots (QDs) were prepared in genetically engineered Escherichia coli (E. coli) through the introduction of foreign genes encoding a CdS binding peptide. The CdS QDs were successfully separated from the bacteria through two methods, lysis and freezing-thawing of cells, and purified with an anion-exchange resin. High-resolution transmission electron microscopy, X-ray diffraction, luminescence spectroscopy, and energy dispersive X-ray spectroscopy were applied to characterize the as-prepared CdS QDs. The effects of reactant concentrations, bacteria incubation times, and reaction times on QD growth were systematically investigated. Our work demonstrates that genetically engineered bacteria can be used to synthesize QDs. The biologically synthesized QDs are expected to be more biocompatible probes in bio-labeling and imaging.
authors: Department of Chemistry, Northeastern University, Shenyang 110819, PR China.
authors: Congcong
authors: C
authors: Mi
authors: Yanyan
authors: Y
authors: Wang
authors: Jingpu
authors: J
authors: Zhang
authors: Huaiqing
authors: H
authors: Huang
authors: Linru
authors: L
authors: Xu
authors: Shuo
authors: S
authors: Wang
authors: Xuexun
authors: X
authors: Fang
authors: Jin
authors: J
authors: Fang
authors: Chuanbin
authors: C
authors: Mao
authors: Shukun
authors: S
authors: Xu
mesh_terms: D019149:Bioreactors / Q000382:microbiology*; D001709:Biotechnology / Q000379:methods*; D019187:Cadmium Compounds / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005818:Genetic Engineering / Q000379:methods; D046529:Microscopy, Electron, Transmission; D010455:Peptides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D045663:Quantum Dots; D013050:Spectrometry, Fluorescence; D013440:Sulfides / Q000737:chemistry*
chemical_list: D019187:Cadmium Compounds; D010455:Peptides; D013440:Sulfides; C034939:cadmium sulfide