title: Acrylamide genotoxicity in young versus adult gpt delta male rats.
abstract: The recent discovery that the potent carcinogen acrylamide (AA) is present in a variety of fried and baked foods raises health concerns, particularly for children, because AA is relatively high in child-favoured foods such as potato chips and French fries. To compare the susceptibility to AA-induced genotoxicity of young versus adult animals, we treated 3- and 11-week-old male gpt delta transgenic F344 rats with 0, 20, 40 or 80 p.p.m. AA via drinking water for 4 weeks and then examined genotoxicity in the bone marrow, liver and testis. We also analysed the level of N7-(2-carbamoyl-2-hydroxyethyl)-guanine (N7-GA-Gua), the major DNA adduct induced by AA, in the liver, testis and mammary gland. At 40 and 80 p.p.m., both age groups yield similar results in the comet assay in liver; but at 80 p.p.m., the bone marrow micronucleus frequency and the gpt-mutant frequency in testis increased significantly only in the young rats, and N7-GA-Gua adducts in the testis was significantly higher in the young rats. These results imply that young rats are more susceptible than adult rats to AA-induced testicular genotoxicity.
authors: Division of Genetics and Mutagenesis, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
authors: Naoki
authors: N
authors: Koyama
authors: Manabu
authors: M
authors: Yasui
authors: Aoi
authors: A
authors: Kimura
authors: Shigeaki
authors: S
authors: Takami
authors: Takuya
authors: T
authors: Suzuki
authors: Kenichi
authors: K
authors: Masumura
authors: Takehiko
authors: T
authors: Nohmi
authors: Shuichi
authors: S
authors: Masuda
authors: Naohide
authors: N
authors: Kinae
authors: Tomonari
authors: T
authors: Matsuda
authors: Toshio
authors: T
authors: Imai
authors: Masamitsu
authors: M
authors: Honma
mesh_terms: D020106:Acrylamide / Q000633:toxicity*; D000375:Aging / Q000187:drug effects*; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D001853:Bone Marrow / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D020552:Comet Assay; D018736:DNA Adducts / Q000378:metabolism; D005260:Female; D008297:Male; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects; D009153:Mutagens / Q000633:toxicity*; D009154:Mutation / Q000235:genetics; D009928:Organ Specificity / Q000187:drug effects; D051381:Rats; D055647:Rats, Transgenic
chemical_list: D018736:DNA Adducts; D009153:Mutagens; D020106:Acrylamide