title: HLA antibodies in pediatric heart transplantation.
abstract: We have analyzed the impact of anti-HLA antibodies present in the patients' circulation prior and/or following heart transplantation in a population of 108 pediatric recipients. Anti-HLA class I and class II antibodies were monitored by traditional CDC using donor and panel T and B lymphocytes and by SPA for detection of DSA. There was a highly significant correlation between the development of AMR and presence of CDC- or SPA-detected DSA. However, the fraction of the transplant population which remained AMR-free was much higher among patients with SPA-detected compared to CDC-detected DSA. Furthermore, long-term graft survival was negatively affected only by cytotoxic, complement-fixing anti-HLA class I antibodies developing following transplantation. Anti-HLA class I or class II antibodies detected by SPA had no effect on long-term survival rates.
authors: Departments of Pathology & Cell Biology Pediatrics, Columbia University, New York, NY 10032, USA.
authors: Eric K
authors: EK
authors: Ho
authors: Elena R
authors: ER
authors: Vasilescu
authors: George
authors: G
authors: Vlad
authors: Charles C
authors: CC
authors: Marboe
authors: Linda J
authors: LJ
authors: Addonizio
authors: Nicole
authors: N
authors: Suciu-Foca
mesh_terms: D000293:Adolescent; D000906:Antibodies / Q000737:chemistry*; D001402:B-Lymphocytes / Q000276:immunology; D001706:Biopsy; D002648:Child; D002675:Child, Preschool; D003165:Complement System Proteins; D004699:Endocardium / Q000473:pathology; D005260:Female; D006333:Heart Failure / Q000628:therapy*; D016027:Heart Transplantation / Q000379:methods*; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D006801:Humans; D016130:Immunophenotyping; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D009206:Myocardium / Q000473:pathology; D010372:Pediatrics; D013997:Time Factors; D016896:Treatment Outcome
chemical_list: D000906:Antibodies; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D003165:Complement System Proteins