title: Antimicrobial susceptibilities of Brucella isolates from various clinical specimens.
abstract: OBJECTIVE
  Brucellosis is a worldwide zoonotic disease and still constitutes a major public health problem. In the study we claimed to identify Brucella species from clinical samples of patients with active brucellosis from Van region of Eastern Anatolia and to determine in vitro antimicrobial susceptibilities of these strains to commonly used anti-Brucella agents and a possible new alternative tigecycline.
  
  
  METHODS
  A total of 56 Brucella isolates were enrolled the study and the identification of the isolates were based on conventional methods. In vitro activities of antimicrobials were evaluated by the E test method.
  
  
  RESULTS
  All isolates were identified as B. melitensis. MIC(90) values of doxycycline, streptomycin, rifampin, trimethoprim-sulfamethoxazole and tigecycline were 0.064 mg/L, 1 mg/L, 2 mg/L, 0.125 mg/L and 0.094 mg/L, respectively. Tigecycline had low MIC(50) and MIC(90) values against all B. melitensis strains; the highest MIC observed was 0.25 μg/mL.
  
  
  CONCLUSIONS
  Our data suggest that tigecycline can be a therapeutic alternative option for the treatment of brucellosis.
authors: Department of Microbiology, Van Education and Research Hospital, 65100 Van, Turkey.
authors: Yasemin
authors: Y
authors: Bayram
authors: Hanifi
authors: H
authors: Korkoca
authors: Cenk
authors: C
authors: Aypak
authors: Mehmet
authors: M
authors: Parlak
authors: Aytekin
authors: A
authors: Cikman
authors: Selcuk
authors: S
authors: Kilic
authors: Mustafa
authors: M
authors: Berktas
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002002:Brucella / Q000187:drug effects* / Q000302:isolation & purification; D017347:Brucella melitensis / Q000187:drug effects / Q000302:isolation & purification; D002006:Brucellosis / Q000188:drug therapy / Q000382:microbiology*; D004318:Doxycycline / Q000494:pharmacology; D006801:Humans; D008826:Microbial Sensitivity Tests; D008911:Minocycline / Q000031:analogs & derivatives / Q000494:pharmacology; D012293:Rifampin / Q000494:pharmacology; D013307:Streptomycin / Q000494:pharmacology; D000078304:Tigecycline; D015662:Trimethoprim, Sulfamethoxazole Drug Combination / Q000494:pharmacology; D014421:Turkey
chemical_list: D000900:Anti-Bacterial Agents; D000078304:Tigecycline; D015662:Trimethoprim, Sulfamethoxazole Drug Combination; D008911:Minocycline; D004318:Doxycycline; D012293:Rifampin; D013307:Streptomycin