title: Enhancement of vascularization and granulation tissue formation by growth factors in human platelet-rich plasma-containing fragmin/protamine microparticles.
abstract: The purpose of this study was to evaluate effects of human platelet-rich plasma (PRP)-containing fragmin/protamine microparticles (F/P MPs) as a protein carrier on neovascularization and granulation tissue formation. Frozen and thawed PRP contains high concentrations of various growth factors (GFs) and F/P MPs effectively adsorb those GFs. Human microvascular endothelial cells (MVECs) and dermal fibroblast cells (DFCs) were optimally grown in medium containing 4% PRP and the addition of F/P MPs significantly maintained and protected the proliferative activity of PRP incubated at 37°C for more than 10 days. When PRP-containing F/P MPs were subcutaneously injected into the back of mice, significant neovascularization was induced near the injected site with enhanced filtration of inflammatory cells from day 3 to day 30, compared with controls (injections of PRP, F/P MPs, and saline). Both PRP-containing F/P MPs and PRP alone induced significant formation of granulation tissue at the injected site. However, thickness of induced granulation tissues was well maintained for 30 days only in PRP-containing F/P MP-injected group. Those bound GFs may be gradually diffused and released from F/P MPs in vitro and in vivo. Thereby, PRP-containing F/P MPs offer significantly higher inductions of vascularization and fibrous tissue formation in vivo than PRP alone.
authors: Department of Plastic Surgery, National Defense Medical College, Saitama, 359-8513, Japan.
authors: Megumi
authors: M
authors: Takikawa
authors: Shin-Ichiro
authors: S
authors: Nakamura
authors: Shingo
authors: S
authors: Nakamura
authors: Masaki
authors: M
authors: Nambu
authors: Masayuki
authors: M
authors: Ishihara
authors: Masanori
authors: M
authors: Fujita
authors: Satoko
authors: S
authors: Kishimoto
authors: Takashi
authors: T
authors: Doumoto
authors: Satoshi
authors: S
authors: Yanagibayashi
authors: Ryuichi
authors: R
authors: Azuma
authors: Naoto
authors: N
authors: Yamamoto
authors: Tomoharu
authors: T
authors: Kiyosawa
mesh_terms: D000818:Animals; D055252:Cell-Derived Microparticles; D002478:Cells, Cultured; D017985:Dalteparin / Q000378:metabolism*; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D006097:Granulation Tissue / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism* / Q000494:pharmacology*; D051379:Mice; D018919:Neovascularization, Physiologic; D053657:Platelet-Rich Plasma / Q000737:chemistry*; D011479:Protamines / Q000378:metabolism*
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D011479:Protamines; D017985:Dalteparin