title: Receptor for Activated C Kinase-1 protein from Penaeus monodon (Pm-RACK1) participates in the shrimp antioxidant response.
abstract: Cellular oxidative stress responses are caused in many ways, but especially by disease and environmental stress. After the initial burst of reactive oxygen species (ROS), the effective elimination of ROS is crucial for the survival of organisms and is mediated by antioxidant defense mechanisms. In this paper, we investigate the possible antioxidant function of Penaeus monodon Receptor for Activated C Kinase-1 (Pm-RACK1). When Pm-RACK1 was over-expressed in Escherichia coli cells or Spodoptera frugiperda (Sf9) insect cells exposed to H(2)O(2), it significantly protected the cells from oxidative damage induced by H(2)O(2). When recombinant Pm-RACK1 protein was expressed as a histidine fusion protein in E. coli and purified with a Ni(2+)-column it possessed antioxidant functions that protected DNA from metal-catalyzed oxidation. Shrimp (Penaeus vannamei) held at an alkaline pH had a much higher hepatopancreatic expression of Pm-RACK1 than in those held at pH 7.4. The exposure of shrimp to alkaline pH is also known to increase ROS production. These results provide strong evidence that Pm-RACK1 can participate in the shrimp antioxidant response induced by the formation of ROS.
authors: Center for Genomics and Bioinformatics Research, Faculty of Science, Prince of Songkla University, Songkhla 90112, Thailand.
authors: Netnapa
authors: N
authors: Saelee
authors: Moltira
authors: M
authors: Tonganunt-Srithaworn
authors: Warapond
authors: W
authors: Wanna
authors: Amornrat
authors: A
authors: Phongdara
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004926:Escherichia coli; D043143:Hepatopancreas / Q000378:metabolism; D006861:Hydrogen Peroxide; D006863:Hydrogen-Ion Concentration; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000502:physiology*; D033561:Penaeidae / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D000075370:Receptors for Activated C Kinase; D011956:Receptors, Cell Surface / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018411:Spodoptera
chemical_list: D017382:Reactive Oxygen Species; D000075370:Receptors for Activated C Kinase; D011956:Receptors, Cell Surface; D006861:Hydrogen Peroxide