title: Estrogen and progesterone receptor isoform distribution through the menstrual cycle in uteri with and without adenomyosis.
abstract: OBJECTIVE
  To test the hypothesis that the expression of the different isoforms of the estrogen receptor alpha (ER-α) and beta (ER-β) and the progesterone receptor A (PR-A) and B (PR-B) would be differentially modulated in uteri with adenomyosis compared with controls and that modulation would be related to the menstrual cycle.
  
  
  METHODS
  Case control, blinded comparison.
  
  
  METHODS
  University department.
  
  
  METHODS
  54 premenopausal women with and 35 without uterine adenomyosis as the sole pathology.
  
  
  METHODS
  Multiple samples studied using immunohistochemistry for estrogen and progesterone receptors.
  
  
  METHODS
  Histomorphometric analysis of receptor expression.
  
  
  RESULTS
  The ER-α expression in the adenomyotic endometrium was different from that of the normal endometrium and the foci in the midsecretory phase of the cycle, but expression of ER-α in the inner and outer myometrium was not statistically significantly different. The ER-β expression was statistically significantly elevated in the adenomyotic functionalis gland during the proliferative phase and throughout the myometrium across the entire menstrual cycle. Expression of PR-A was similar to that of PR-B, with reduced expression in the basalis stroma, and inner and outer myometrium in the adenomyotic samples. The pattern of ER-β, PR-A, and PR-B expression was similar in the endometrial basalis and adenomyotic foci.
  
  
  CONCLUSIONS
  These data suggest ER-β expression and the lack of PR expression are related to the development and/or progression of adenomyosis and might explain the poor response of adenomyosis-associated menstrual symptoms to progestational agents.
authors: Reproductive Sciences Section, Department of Cancer Studies and Molecular Medicine, University of Leicester, and Histopathology Department, Leicester Royal Infirmary, University Hospital of Leicester NHS Trust, Leicester, United Kingdom. mohamed.mehasseb@doctors.net.uk
authors: Mohamed Khairy
authors: MK
authors: Mehasseb
authors: Rina
authors: R
authors: Panchal
authors: Anthony Henry
authors: AH
authors: Taylor
authors: Laurence
authors: L
authors: Brown
authors: Stephen Charles
authors: SC
authors: Bell
authors: Marwan
authors: M
authors: Habiba
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D004715:Endometriosis / Q000378:metabolism* / Q000503:physiopathology; D004739:England; D047628:Estrogen Receptor alpha / Q000378:metabolism*; D047629:Estrogen Receptor beta / Q000378:metabolism*; D005260:Female; D006785:Hospitals, University; D006801:Humans; D007150:Immunohistochemistry; D008597:Menstrual Cycle; D011980:Receptors, Progesterone / Q000378:metabolism*; D014591:Uterine Diseases / Q000378:metabolism* / Q000503:physiopathology; D014599:Uterus / Q000378:metabolism* / Q000503:physiopathology
chemical_list: C506487:ESR1 protein, human; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D011980:Receptors, Progesterone