title: [Effect of grape procyanidins on expressions of Caspase-3 and Bax in ethanol-induced liver injury in mice].
abstract: OBJECTIVE
  To study the effect of grape procyanidins (GPC) on the expressions of Caspase-3 and Bax in the liver of mice with ethanol-induced liver injury.
  
  
  METHODS
  The mice were orally given at the different doses of GPC (50,100 and 200 mg/kg) and 4 g/kg ethanol, and killed after 4 weeks. The proliferation activity of the hepatic cells was determined by MTT assay,the levels of Caspase-3 and Bax protein expression in the cells were measured by immunohistochemistry method.
  
  
  RESULTS
  The expression level of Caspase-3 and Bax protein in high dose group were 19.08% and 14.06%, respectively, and those of the ethanol-injured group were 51.78% and 58.08%. The differences between the two groups were significant (P < 0.05). The cell proliferation activity of high dose and medium dose group was higher than the cell proliferation activity of ethanol-injured group (P < 0.05).
  
  
  CONCLUSIONS
  GPC could suppressing the expression of Caspase-3 and Bax in the liver of mice with ethanol-induced liver injury.
authors: Medical College of Qingdao University, Qingdao 266021, China. lj.112004@163.com
authors: Jing
authors: J
authors: Li
authors: Jinyi
authors: J
authors: Zhong
authors: Xiao
authors: X
authors: Qi
authors: Yanling
authors: Y
authors: Yin
authors: Zheling
authors: Z
authors: Wang
mesh_terms: D000818:Animals; D053148:Caspase 3 / Q000378:metabolism*; D000431:Ethanol / Q000008:administration & dosage; D005260:Female; D056604:Grape Seed Extract / Q000737:chemistry*; D008099:Liver / Q000378:metabolism; D008108:Liver Diseases, Alcoholic / Q000188:drug therapy / Q000378:metabolism*; D008297:Male; D051379:Mice; D044945:Proanthocyanidins / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D051028:bcl-2-Associated X Protein / Q000378:metabolism*
chemical_list: C494827:Bax protein, mouse; D056604:Grape Seed Extract; D044945:Proanthocyanidins; D051028:bcl-2-Associated X Protein; D000431:Ethanol; C505409:Casp3 protein, mouse; D053148:Caspase 3