title: Examination of the inflammatory response following implantation of titanium plates coated with phospholipids in rats.
abstract: Implantation of biomaterials like titanium (Ti) causes inflammatory reactions possibly affecting implant functionality. Surface modifications could improve biocompatibility and functionality of implants. Biomembrane-derived phospholipids might be useful as implant coating due to their biomimetic properties. In vitro studies demonstrated beneficial effects for 2-oleoyl-1-palmitoyl-sn-glycero-3-phosphoethanolamin (POPE) as coating regarding interactions with cells and bacteria. Therefore, this in vivo study aimed at examining local inflammatory reactions after implantation of POPE-coated Ti plates. Ti implants with POPE attached non-covalently or covalent via octadecylphosphonic acid (OPA), with OPA alone and uncoated controls were simultaneously implanted intramuscularly in rats for 7, 14 and 56 days. The peri-implant tissue was quantitatively analyzed by immunohistochemistry for total macrophages, tissue macrophages, T cells, antigen-presenting cells and proliferating cells. Overall, both POPE-coated series were comparable to the controls. Furthermore, no differences were found between POPE coating on a covalently linked OPA monolayer and POPE coating dried from solution. Together with earlier in vitro results, this demonstrates the potential of phospholipids for implant surface modification.
authors: Department of Medical Biochemistry and Molecular Biology, Research Group of Predictive Diagnostics, Ernst Moritz Arndt University of Greifswald, Greifswalder Str 11c, 17495 Karlsburg, Germany.
authors: Alexandra
authors: A
authors: Kochanowski
authors: Andreas
authors: A
authors: Hoene
authors: Maciej
authors: M
authors: Patrzyk
authors: Uwe
authors: U
authors: Walschus
authors: Birgit
authors: B
authors: Finke
authors: Bérengère
authors: B
authors: Luthringer
authors: Frank
authors: F
authors: Feyerabend
authors: Regine
authors: R
authors: Willumeit
authors: Silke
authors: S
authors: Lucke
authors: Michael
authors: M
authors: Schlosser
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D032701:Biomimetics; D049109:Cell Proliferation; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D007249:Inflammation; D008264:Macrophages / Q000166:cytology; D008422:Materials Testing; D063065:Organophosphonates / Q000737:chemistry; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry*; D051381:Rats; D044968:Regenerative Medicine / Q000379:methods; D013997:Time Factors; D014025:Titanium / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D063065:Organophosphonates; D010714:Phosphatidylethanolamines; D010743:Phospholipids; C057561:1-palmitoyl-2-oleoylphosphatidylethanolamine; D014025:Titanium