title: Detection of HTLV-1 by means of HBZ gene in situ hybridization in formalin-fixed and paraffin-embedded tissues.
abstract: Adult T-cell leukemia/lymphoma (ATLL) is a T-cell malignancy associated with HTLV-1. The HTLV-1 provirus genome has the pX region that encodes tax and HTLV-1 basic leucine zipper factor (HBZ). Previous studies have reported that the tax gene is expressed in few ATLL cases, but the HBZ gene in all ATLL cases. In this study, we used HBZ gene in situ hybridization (HBZ-ISH) for detection of the HBZ gene in formalin-fixed paraffin-embedded tissues. This method showed that all cases (n = 19) were positive for the ATLL cell line (MT-1, MT-2, and MT-4) and ATLL mouse model (HBZ-Tg mice and NOD/SCID/β2-microglobulin(null) mice with ATLL transplanted), and the HBZ gene was also detected in all human ATLL cases (n = 16). The percentage of positive cells in HBZ-ISH was 5-70%. Immunohistochemical staining for Tax protein showed positivity in seven of 11 cases in NOD/SCID/β2-microglobulin(null) mice with ATLL transplanted and in six of eight human ATLL cases, but the percentage of positive cells was very low (range, 1-5%). Although HBZ-ISH is unsuitable to detect HTLV-1 clonality, this method is convenient and can be useful for the histological diagnosis of ATLL in HTLV-1 sero-indeterminate patients.
authors: Department of Pathology, School of Medicine, Kurume University, Kurume, Japan.
authors: Kei
authors: K
authors: Shimizu-Kohno
authors: Yorifumi
authors: Y
authors: Satou
authors: Fumiko
authors: F
authors: Arakawa
authors: Junichi
authors: J
authors: Kiyasu
authors: Yoshizo
authors: Y
authors: Kimura
authors: Daisuke
authors: D
authors: Niino
authors: Yasuo
authors: Y
authors: Sugita
authors: Fumihiko
authors: F
authors: Ishikawa
authors: Masao
authors: M
authors: Matsuoka
authors: Koichi
authors: K
authors: Ohshima
mesh_terms: D000818:Animals; D050976:Basic-Leucine Zipper Transcription Factors / Q000235:genetics*; D005557:Formaldehyde; D016356:Gene Products, tax / Q000032:analysis; D015368:Human T-lymphotropic virus 1 / Q000302:isolation & purification*; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization / Q000379:methods*; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000175:diagnosis* / Q000821:virology; D051379:Mice; D016513:Mice, SCID; D010232:Paraffin; D012191:Retroviridae Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014764:Viral Proteins / Q000235:genetics*; D001613:beta 2-Microglobulin / Q000502:physiology
chemical_list: D050976:Basic-Leucine Zipper Transcription Factors; D016356:Gene Products, tax; C479751:HBZ protein, human T-cell leukemia virus type I; D012191:Retroviridae Proteins; D014764:Viral Proteins; D001613:beta 2-Microglobulin; C514970:tax protein, Human T-lymphotrophic virus 1; D005557:Formaldehyde; D010232:Paraffin