title: Apoptin sensitizes radiation-induced cell death via classic mitochondrial, caspase and p53-dependent signaling in HepG2 cells.
abstract: Resistance or insensitivity to radiation therapy is one of the hallmarks of hepatocellular carcinoma. Sensitizing radioresistant cancer by combining radiation with other therapeutics to induce apoptosis has been widely investigated. Our previous study showed that chicken anaemia virus-derived apoptin protein induced the apoptosis of hepatic carcinoma HepG2 cells. In the present study, we demonstrated that apoptin sensitizes cells to radiation-induced apoptosis using a lentivirus-apoptin expression system in hepatic carcinoma HepG2 cells. Combination therapy with radiation and apoptin dramatically induced mitochondrial cytochrome c release and the cleavage of caspases -9, -3 and -7. Our findings are also the first to show that the combination of radiation and apoptin up-regulates p53 expression. Thus, apoptin treatment represents a potential method for enhancing the effectiveness of radiotherapy in poorly responding hepatocellular carcinoma.
authors: Department of Oncology, The First Affiliated Hospital of Xi'an Jiaotong University College of Medicine, Xi'an 710061, PR China. hsummer22099@yahoo.cn
authors: Su-Xia
authors: SX
authors: Han
authors: Qing
authors: Q
authors: Zhu
authors: Jin-Lu
authors: JL
authors: Ma
authors: Yi
authors: Y
authors: Lv
authors: Jing
authors: J
authors: Zhao
authors: Chen
authors: C
authors: Huang
authors: Xi
authors: X
authors: Jia
authors: Wei
authors: W
authors: Ou
authors: Hong-Tao
authors: HT
authors: Guo
mesh_terms: D036022:Capsid Proteins / Q000008:administration & dosage / Q000627:therapeutic use*; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy / Q000532:radiotherapy*; D020169:Caspases / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects / Q000528:radiation effects; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy / Q000532:radiotherapy*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D011838:Radiation-Sensitizing Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: D036022:Capsid Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D011838:Radiation-Sensitizing Agents; D016159:Tumor Suppressor Protein p53; C091717:VP3 protein, Chicken anemia virus; D020169:Caspases