title: Long-term outcome of cryopreserved bone-derived osteoblasts for bone regeneration in vivo.
abstract: Cryopreserved bone-derived osteoblasts (CBOs) have been considered as a promising cell source for bone regeneration. Previous studies have demonstrated that CBOs had good proliferation and osteogenicity. However, the long-term outcome of CBOs in vivo still remains unknown. In this experiment, we applied CBOs combined with calcium phosphate cement (CPC) to augment maxillary sinus in canine, computer tomography, polychrome labeling, biomechanical tests, fluorescent immunohistochemistry staining and histological analysis were used to analyze the property and mineralization process of the tissue-engineered bone preclinical application. Our results showed that CBOs combined with CPC could promote bone regeneration, dramatically maintain the height, volume and biomechanical property of augmented maxillary sinus. Furthermore, the tissue-engineered bone was more mature than scaffold alone or autogenous bone, and bone formation and remodeling were still apparent 20 months postoperatively. Additionally, 4 months after surgery might be the suitable time point for implants placement in the regenerated bone. These results also indicate that cryopreserved bone may be a potential source of osteoblasts for maxillary sinus augmentation.
authors: Department of Oral and Maxillofacial Surgery, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai, China.
authors: Shaoyi
authors: S
authors: Wang
authors: Wenjie
authors: W
authors: Zhang
authors: Jun
authors: J
authors: Zhao
authors: Dongxia
authors: D
authors: Ye
authors: Chao
authors: C
authors: Zhu
authors: Yunhao
authors: Y
authors: Yang
authors: Xiuli
authors: X
authors: Zhang
authors: Xiaojuan
authors: X
authors: Sun
authors: Chi
authors: C
authors: Yang
authors: Xinquan
authors: X
authors: Jiang
authors: Zhiyuan
authors: Z
authors: Zhang
mesh_terms: D000540:Alveolar Ridge Augmentation / Q000379:methods; D000818:Animals; D001861:Bone Regeneration / Q000502:physiology*; D002478:Cells, Cultured; D015925:Cryopreservation; D004285:Dogs; D008297:Male; D008422:Materials Testing; D008443:Maxillary Sinus / Q000473:pathology / Q000601:surgery; D013516:Oral Surgical Procedures, Preprosthetic / Q000379:methods; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry; D014057:Tomography, X-Ray Computed