title: Inhibitory effect of a water extract from Pemphis acidula on melanogenesis in mouse B16 melanoma cells.
abstract: The inhibitory effect of a water extract from Pemphis acidula on melanogenesis in mouse B16 melanoma cells was investigated. The results showed that the P. acidula extract (PAE) inhibited melanogenesis in 3-isobutyl-1-methylxanthin (IBMX)-stimulated B16 cells in a dose-dependent manner, with an IC50 value of 33.5 μg/ml. In addition, PAE also inhibited cellular tyrosinase activity. Moreover, western blot and real-time reverse transcriptase polymerase chain reaction (qRT-PCR) analyses respectively confirmed that PAE down-regulated levels of tyrosinase protein and its mRNA in IBMX-stimulated B16 cells. These results demonstrated that PAE inhibits melanogenesis of B16 cells by reducing tyrosinase gene expression. From the present study, PAE is proven to be a good candidate as a skin-whitening agent for treatment of skin hyperpigmentation.
authors: Institute of Cosmetics Science, Chia Nan University of Pharmacy and Science, National University of Tainan, Taiwan, ROC.
authors: Hsiou-Yu
authors: HY
authors: Ding
authors: Te-Sheng
authors: TS
authors: Chang
authors: Chien-Min
authors: CM
authors: Chiang
authors: Sorgan
authors: S
authors: Shou-Ku Tai
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D015971:Gene Expression Regulation, Enzymologic; D029561:Lythraceae / Q000737:chemistry*; D008543:Melanins / Q000378:metabolism; D051379:Mice; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors / Q000378:metabolism; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014867:Water / Q000737:chemistry*
chemical_list: D008543:Melanins; D010936:Plant Extracts; D012333:RNA, Messenger; D014867:Water; D014442:Monophenol Monooxygenase