title: [Empirical study of reverse effect on mediated multidrug resistance of U-2OS/ADM cells with curcumin in vitro].
abstract: OBJECTIVE
  To investigate the reversion of P-gp mediated multidrug resistance of U-2OS/ADM cells with curcumin in vitro.
  
  
  METHODS
  With doxorubicin as induction drugs, human osteosarcoma cell line U-2OS was induced object, multi-drug resistant of human osteosarcoma cell line model (U-2OS/ADM) were established by high-dose method, The before and after reversal efficacy of curcumin for U-2OS/ADM cells to chemotherapeutic drugs were measured by MTT assay. The effects of curcumin on Rh-123 uptake and efflux were analyzed by flow cytometer.
  
  
  RESULTS
  (1) MTT demonstrates curcumin (20 micromol/L) can increase the cytotoxicity of Adriamycin to U-2OS/ADM cells. (2) The result of FCM shows that Curcumin can increase the accumulation of Rh-123 and increase the cytotoxicity of Adriamycin to U-2OS/ADM cells in a dose-dependent manner.
  
  
  CONCLUSIONS
  The reversal mechanism of curcumin was blocked the function of P-gp in U-2OS/ADM cellular membrane.
authors: Loudi Municipal Central Hospital of Hunan Province, Loudi 417000, China. Xiao_Yang168@sina.com
authors: Yang
authors: Y
authors: Xiao
authors: Wanchun
authors: W
authors: Wang
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D001859:Bone Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D003474:Curcumin / Q000494:pharmacology*; D004317:Doxorubicin / Q000494:pharmacology; D018432:Drug Resistance, Multiple / Q000187:drug effects*; D019008:Drug Resistance, Neoplasm / Q000187:drug effects*; D006801:Humans; D012516:Osteosarcoma / Q000473:pathology*
chemical_list: D000970:Antineoplastic Agents; D004317:Doxorubicin; D003474:Curcumin