title: Osthole induces G2/M arrest and apoptosis in lung cancer A549 cells by modulating PI3K/Akt pathway.
abstract: BACKGROUND
  To explore the effects of Osthole on the proliferation, cell cycle and apoptosis of human lung cancer A549 cells.
  
  
  METHODS
  Human lung cancer A549 cells were treated with Osthole at different concentrations. Cell proliferation was measured using the MTT assay. Cell cycle was evaluated using DNA flow cytometry analysis. Induction of apoptosis was determined by flow cytometry and fluorescent microscopy. The expressions of Cyclin B1, p-Cdc2, Bcl-2, Bax, t-Akt and p-Akt were evaluated by Western blotting.
  
  
  RESULTS
  Osthole inhibited the growth of human lung cancer A549 cells by inducing G2/M arrest and apoptosis. Western blotting demonstrated that Osthole down-regulated the expressions of Cyclin B1, p-Cdc2 and Bcl-2 and up-regulated the expressions of Bax in A549 cells. Inhibition of PI3K/Akt signaling pathway was also observed after treating A549 cells with Osthole.
  
  
  CONCLUSIONS
  Our findings suggest that Osthole may have a therapeutic application in the treatment of human lung cancer.
authors: Department of Respiratory Medicine, the Shengjing Hospital, China Medical University, Shenyang 110004, PR China.
authors: Xiaoman
authors: X
authors: Xu
authors: Yi
authors: Y
authors: Zhang
authors: Dan
authors: D
authors: Qu
authors: Tingshu
authors: T
authors: Jiang
authors: Shengqi
authors: S
authors: Li
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D016203:CDC2 Protein Kinase; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003374:Coumarins / Q000494:pharmacology*; D019926:Cyclin B / Q000378:metabolism; D056744:Cyclin B1 / Q000378:metabolism; D018844:Cyclin-Dependent Kinases; D004354:Drug Screening Assays, Antitumor; D016195:G2 Phase / Q000187:drug effects*; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D051058:Oncogene Protein v-akt / Q000378:metabolism*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects*; D051025:bcl-Associated Death Protein / Q000378:metabolism
chemical_list: D003374:Coumarins; D019926:Cyclin B; D056744:Cyclin B1; D051025:bcl-Associated Death Protein; D019869:Phosphatidylinositol 3-Kinases; D051058:Oncogene Protein v-akt; D016203:CDC2 Protein Kinase; C528899:CDK1 protein, human; D018844:Cyclin-Dependent Kinases; C046627:osthol