title: Facile synthesis of monodispersed mesoporous silica nanoparticles with ultralarge pores and their application in gene delivery.
abstract: Among various nanoparticles, the silica nanoparticle (SiNP) is an attractive candidate as a gene delivery carrier due to advantages such as availability in porous forms for encapsulation of drugs and genes, large surface area to load biomacromolecules, biocompatibility, storage stability, and easy preparation in large quantity with low cost. Here, we report on a facile synthesis of monodispersed mesoporous silica nanoparticles (MMSN) possessing very large pores (>15 nm) and application of the nanoparticles to plasmid DNA delivery to human cells. The aminated MMSN with large pores provided a higher loading capacity for plasmids than those with small pores (∼2 nm), and the complex of MMSN with plasmid DNA readily entered into cells without supplementary polymers such as cationic dendrimers. Furthermore, MMSN with large pores could efficiently protect plasmids from nuclease-mediated degradation and showed much higher transfection efficiency of the plasmids encoding luciferase and green fluorescent protein (pLuc, pGFP) compared to MMSN with small pores (∼2 nm).
authors: Department of Chemistry, KAIST, Daejeon 305-701, Korea.
authors: Mi-Hee
authors: MH
authors: Kim
authors: Hee-Kyung
authors: HK
authors: Na
authors: Young-Kwan
authors: YK
authors: Kim
authors: Soo-Ryoon
authors: SR
authors: Ryoo
authors: Hae Sung
authors: HS
authors: Cho
authors: Kyung Eun
authors: KE
authors: Lee
authors: Hyesung
authors: H
authors: Jeon
authors: Ryong
authors: R
authors: Ryoo
authors: Dal-Hee
authors: DH
authors: Min
mesh_terms: D004058:Diffusion; D006367:HeLa Cells; D006801:Humans; D008422:Materials Testing; D053769:Nanocapsules / Q000737:chemistry*; D010957:Plasmids / Q000008:administration & dosage / Q000737:chemistry* / Q000235:genetics*; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*; D014162:Transfection / Q000379:methods*
chemical_list: D053769:Nanocapsules; D012822:Silicon Dioxide