title: Interleukin-1β inhibits voltage-gated sodium currents in a time- and dose-dependent manner in cortical neurons.
abstract: Interleukin-1β (IL-1β) is a multifunctional proinflammatory cytokine that plays a key role in the injuries and diseases of the central nervous system (CNS). A voltage-gated Na(+) channel is essential for the excitability and electrical properties of neurons. However, it is not known whether IL-1β directly affects the central Na(+) channels. In the present study, we examined the effects of IL-1β on Na(+) currents in cultured cortical neurons using patch-clamp recording. Our results showed that IL-1β suppressed Na(+) currents through its receptor in a time- and dose-dependent manner, but did not alter the voltage-dependent activation and inactivation. PKC and then p38 MAPK were involved in this inhibition. The spike amplitude was also inhibited by IL-1β in the doses that decreased the Na(+) currents. Our findings revealed the inhibition of chronic IL-1β treatment on voltage-gated Na(+) channels in the CNS, and showed that the action potential (AP) amplitude was reduced by IL-1β due to a decrease of Na(+) currents.
authors: College of Life Sciences, Hebei University, 180 Wusidong Rd, Baoding, Hebei 071002, People's Republic of China. zezhou@gmail.com
authors: Chen
authors: C
authors: Zhou
authors: Cui
authors: C
authors: Qi
authors: Juanjuan
authors: J
authors: Zhao
authors: Fei
authors: F
authors: Wang
authors: Weiwei
authors: W
authors: Zhang
authors: Chen
authors: C
authors: Li
authors: Junzhan
authors: J
authors: Jing
authors: Xianjiang
authors: X
authors: Kang
authors: Zhen
authors: Z
authors: Chai
mesh_terms: D000818:Animals; D001483:Base Sequence; D002540:Cerebral Cortex / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D004247:DNA; D004305:Dose-Response Relationship, Drug; D006168:Guinea Pigs; D053583:Interleukin-1beta / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D016133:Polymerase Chain Reaction; D051381:Rats; D017207:Rats, Sprague-Dawley; D015222:Sodium Channels / Q000502:physiology*
chemical_list: D053583:Interleukin-1beta; D015222:Sodium Channels; D004247:DNA