title: Establishment of clonal cell lines of taste buds from a p53(-/-) mouse tongue.
abstract: A taste bud is a sensory organ and consists of 50-100 spindle-shaped cells. The cells function as taste acceptors. They have characteristics of both epithelial and neuronal cells. A taste bud contains four types of cells, type I, type II, type III cells, and basal cells. Taste buds were isolated from a tongue of a p53-deficient mouse at day 12, and 11 clonal taste bud (TBD) cell lines were established. In immunochemical analysis, all cell lines expressed cytokeratin 18, gustducin, T1R3, and neural cellular adhesion molecule, but not GLAST. In RT-PCR analysis, shh was not expressed in any of the cell lines. Further analysis with RT-PCR was conducted on four cell lines. They expressed G protein-coupled taste receptors; T1R3, T2R8 for sweet, bitter, umami. And they also expressed α-ENaC for salty taste. While, a candidate for sour receptor HCN4 was expressed in TBD-a1 and TBD-a7 lines. And another candidate for sour receptor PKD1L3 was slightly expressed in TBD-a1 and TBD-c1.
authors: Department of Biological Science and Technology and Research Center for RNA Science, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan.
authors: Hideyuki
authors: H
authors: Sako
authors: Makie
authors: M
authors: Hori
authors: Ikuo
authors: I
authors: Masuho
authors: Osamu
authors: O
authors: Saitoh
authors: Atsumasa
authors: A
authors: Okada
authors: Yasuhiro
authors: Y
authors: Tomooka
mesh_terms: D000818:Animals; D015153:Blotting, Western; D015220:Calcium Channels; D002460:Cell Line; D017931:DNA Primers / Q000235:genetics; D027341:Excitatory Amino Acid Transporter 1 / Q000378:metabolism; D007150:Immunohistochemistry; D053538:Keratin-18 / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D019006:Neural Cell Adhesion Molecules / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050396:TRPP Cation Channels / Q000378:metabolism; D013650:Taste Buds / Q000166:cytology*; D014059:Tongue / Q000166:cytology*; D015293:Transducin / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
chemical_list: D015220:Calcium Channels; D017931:DNA Primers; D027341:Excitatory Amino Acid Transporter 1; D053538:Keratin-18; D019006:Neural Cell Adhesion Molecules; C512453:PKD1L3 protein, mouse; D043562:Receptors, G-Protein-Coupled; C493393:Slc1a3 protein, mouse; D050396:TRPP Cation Channels; D016159:Tumor Suppressor Protein p53; C075192:gustducin; D015293:Transducin