title: Rapid detection of anti-Vaccinia virus neutralizing antibodies.
abstract: Increasing infections with Monkeypox and Cowpox viruses pose a continuous and growing threat to human health. The standard method for detecting poxvirus neutralizing antibodies is the plaque-reduction neutralization test that is specific but also time-consuming and laborious. Therefore, a rapid and reliable method was developed to determine neutralizing antibody titers within twelve hours. The new assay measures viral mRNA transcription as a marker for actively replicating virus after incomplete neutralization using real-time PCR.
authors: Robert Koch-Institute, Centre for Biological Safety, Nordufer 20, 13353 Berlin, Germany.
authors: Marit
authors: M
authors: Kramski
authors: Anna
authors: A
authors: Drozd
authors: Gregor F
authors: GF
authors: Lichtfuss
authors: Piotr W
authors: PW
authors: Dabrowski
authors: Heinz
authors: H
authors: Ellerbrok
mesh_terms: D057134:Antibodies, Neutralizing / Q000032:analysis* / Q000276:immunology; D000914:Antibodies, Viral / Q000032:analysis* / Q000276:immunology; D002460:Cell Line; D006801:Humans; D009500:Neutralization Tests; D016133:Polymerase Chain Reaction / Q000379:methods*; D014615:Vaccinia / Q000175:diagnosis* / Q000276:immunology; D014616:Vaccinia virus / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification / Q000502:physiology; D014779:Virus Replication
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral