title: Bone healing in critical-size defects treated with either bone graft, membrane, or a combination of both materials: a histological and histometric study in rat tibiae.
abstract: OBJECTIVE
  The aim of the present investigation was to histologically analyze the effect of using lyophilized bovine bone (GenOx® organic matrix) with (or without) guided tissue regeneration (using a decalcified cortical osseous membrane [GenDerm®]) on bone healing in surgically created critical-size defects created in rat tibia.
  
  
  METHODS
  Surgical critical-size bone defects were created in 64 animals that were randomly divided into four groups: group I (control); group II (defect filled with GenOx®); group III (defect covered by GenDerm®); group IV (defect filled with GenOx® and covered by GenDerm®). Animals were killed at 30 or 90 days post-surgery. The specimens were embedded in paraffin, serially cut, and stained with hematoxylin and eosin for analysis under light microscopy. The formation of new bone in the cortical area of the defect was histomorphometrically evaluated.
  
  
  RESULTS
  All experimental groups demonstrated superior bone healing compared with the control group. However, group IV samples showed evidence of more advanced healing at both 30 and 90 days post-surgery as compared with the other experimental groups.
  
  
  CONCLUSIONS
  The bovine organic bone graft GenOx® associated with GenDerm® this produced the best treatment results in the case of critical-size defects in rat tibia.
authors: Department of Endodontics, Araçatuba School of Dentistry, Univ Estadual Paulista, Araçatuba, SP, Brazil.
authors: Pedro Felício Estrada
authors: PF
authors: Bernabé
authors: Luiz Gustavo Nascimento
authors: LG
authors: Melo
authors: Luciano Tavares Angelo
authors: LT
authors: Cintra
authors: João Eduardo
authors: JE
authors: Gomes-Filho
authors: Eloi
authors: E
authors: Dezan
authors: Maria José Hitomi
authors: MJ
authors: Nagata
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001861:Bone Regeneration / Q000502:physiology*; D018786:Bone Substitutes / Q000494:pharmacology*; D016025:Bone Transplantation / Q000379:methods*; D001842:Bone and Bones; D002417:Cattle; D048091:Guided Tissue Regeneration / Q000379:methods*; D008297:Male; D008567:Membranes, Artificial; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013977:Tibia / Q000601:surgery*; D014020:Tissue Extracts / Q000494:pharmacology*; D014945:Wound Healing / Q000502:physiology*
chemical_list: D018786:Bone Substitutes; D008567:Membranes, Artificial; D014020:Tissue Extracts