title: Regulation of cellular senescence by the essential caveolar component PTRF/Cavin-1.
abstract: Polymerase I and transcript release factor (PTRF, also known as Cavin-1) is an essential component in the biogenesis and function of caveolae. Here, we show that PTRF expression is increased in senescent human fibroblasts. Importantly, overexpression of PTRF induced features characteristic of cellular senescence, whereas reduced PTRF expression extended the cellular replicative lifespan. Interestingly, we found that PTRF localized primarily to the nuclei of young and quiescent WI-38 human fibroblasts, but translocated to the cytosol and plasma membrane during cellular senescence. Furthermore, electron microscopic analysis demonstrated an increased number of caveolar structures in senescent and PTRF-transfected WI-38 cells. Our data suggest that the role of PTRF in cellular senescence is dependent on its targeting to caveolae and its interaction with caveolin-1, which appeared to be regulated by the phosphorylation of PTRF. Taken together, our findings identify PTRF as a novel regulator of cellular senescence that acts through the p53/p21 and caveolar pathways.
authors: Key Lab of Cell Proliferation and Regulation Biology of the Ministry of Education, Institute of Cell Biology, Beijing Normal University, 19 Xin Jie Kou Wai Avenue, Beijing 100875, China.
authors: Lin
authors: L
authors: Bai
authors: Xiaoli
authors: X
authors: Deng
authors: Juanjuan
authors: J
authors: Li
authors: Miao
authors: M
authors: Wang
authors: Qian
authors: Q
authors: Li
authors: Wei
authors: W
authors: An
authors: Deli
authors: D
authors: A
authors: Yu-Sheng
authors: YS
authors: Cong
mesh_terms: D021941:Caveolae / Q000648:ultrastructure; D002460:Cell Line; D016922:Cellular Senescence; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D005786:Gene Expression Regulation; D006801:Humans; D009154:Mutation; D016601:RNA-Binding Proteins / Q000032:analysis* / Q000235:genetics* / Q000378:metabolism
chemical_list: C497835:CAVIN1 protein, human; D016601:RNA-Binding Proteins