title: The DEK oncoprotein is a Su(var) that is essential to heterochromatin integrity.
abstract: Heterochromatin integrity is crucial for genome stability and regulation of gene expression, but the factors involved in mammalian heterochromatin biology are only incompletely understood. Here we identify the oncoprotein DEK, an abundant nuclear protein with a previously enigmatic in vivo function, as a Suppressor of Variegation [Su(var)] that is crucial to global heterochromatin integrity. We show that DEK interacts directly with Heterochromatin Protein 1 α (HP1α) and markedly enhances its binding to trimethylated H3K9 (H3K9me3), which is key for maintaining heterochromatic regions. Loss of Dek in Drosophila leads to a Su(var) phenotype and global reduction in heterochromatin. Thus, these findings show that DEK is a key factor in maintaining the balance between heterochromatin and euchromatin in vivo.
authors: Division of Infectious Diseases, Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor, Michigan 48109, USA.
authors: Ferdinand
authors: F
authors: Kappes
authors: Tanja
authors: T
authors: Waldmann
authors: Veena
authors: V
authors: Mathew
authors: Jindan
authors: J
authors: Yu
authors: Ling
authors: L
authors: Zhang
authors: Michael S
authors: MS
authors: Khodadoust
authors: Arul M
authors: AM
authors: Chinnaiyan
authors: Karolin
authors: K
authors: Luger
authors: Sylvia
authors: S
authors: Erhardt
authors: Robert
authors: R
authors: Schneider
authors: David M
authors: DM
authors: Markovitz
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D000090266:Chromobox Protein Homolog 5; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000378:metabolism*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D055785:Gene Knockdown Techniques; D042822:Genomic Instability / Q000235:genetics; D057809:HEK293 Cells; D006367:HeLa Cells; D006570:Heterochromatin / Q000235:genetics* / Q000378:metabolism*; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology; D006657:Histones / Q000378:metabolism; D006801:Humans; D046529:Microscopy, Electron, Transmission; D015513:Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D000075223:Poly-ADP-Ribose Binding Proteins; D011485:Protein Binding; D036081:Receptors, Eph Family / Q000235:genetics / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: C000715594:CBX5 protein, human; D002868:Chromosomal Proteins, Non-Histone; C084738:DEK protein, human; D029721:Drosophila Proteins; D006570:Heterochromatin; D056572:Histone Deacetylase Inhibitors; D006657:Histones; D015513:Oncogene Proteins; D000075223:Poly-ADP-Ribose Binding Proteins; D000090266:Chromobox Protein Homolog 5; C119557:Dek protein, Drosophila; D036081:Receptors, Eph Family