title: Plasmodium Cysteine Repeat Modular Proteins 3 and 4 are essential for malaria parasite transmission from the mosquito to the host.
abstract: BACKGROUND
  The Plasmodium Cysteine Repeat Modular Proteins (PCRMP) are a family of four conserved proteins of malaria parasites, that contain a number of motifs implicated in host-parasite interactions. Analysis of mutants of the rodent parasite Plasmodium berghei lacking expression of PCRMP1 or 2 showed that these proteins are essential for targeting of P. berghei sporozoites to the mosquito salivary gland and, hence, for transmission from the mosquito to the mouse.
  
  
  METHODS
  In this work, the role of the remaining PCRMP family members, PCRMP3 and 4, has been investigated throughout the Plasmodium life cycle by generation and analysis of P. berghei gene deletion mutants, Δpcrmp3 and Δpcrmp4. The role of PCRMP members during the transmission and hepatic stages of the Plasmodium lifecycle has been evaluated by light- and electron microscopy and by analysis of liver stage development in HEPG2 cells in vitro and by infecting mice with mutant sporozoites. In addition, mice were immunized with live Δpcrmp3 and Δpcrmp4 sporozoites to evaluate their immunization potential as a genetically-attenuated parasite-based vaccine.
  
  
  RESULTS
  Disruption of pcrmp3 and pcrmp4 in P. berghei revealed that they are also essential for transmission of the parasite through the mosquito vector, although acting in a distinct way to pbcrmp1 and 2. Mutants lacking expression of PCRMP3 or PCRMP4 show normal blood stage development and oocyst formation in the mosquito and develop into morphologically normal sporozoites, but these have a defect in egress from oocysts and do not enter the salivary glands. Sporozoites extracted from oocysts perform gliding motility and invade and infect hepatocytes but do not undergo further development and proliferation. Furthermore, the study shows that immunization with Δcrmp3 and Δcrmp4 sporozoites does not confer protective immunity upon subsequent challenge.
  
  
  CONCLUSIONS
  PCRMP3 and 4 play multiple roles during the Plasmodium life cycle; they are essential for the establishment of sporozoite infection in the mosquito salivary gland, and subsequently for development in hepatocytes. However, although Δpcrmp3 and Δpcrmp4 parasites are completely growth-impaired in the liver, immunization with live sporozoites does not induce the protective immune responses that have been shown for other genetically-attenuated parasites.
authors: Malaria Unit, Instituto de Medicina Molecular, Faculdade de Medicina de Lisboa, Av. Prof. Egas Moniz, 1649-028 Lisbon, Portugal.
authors: Bruno
authors: B
authors: Douradinha
authors: Kevin D
authors: KD
authors: Augustijn
authors: Sally G
authors: SG
authors: Moore
authors: Jai
authors: J
authors: Ramesar
authors: Maria M
authors: MM
authors: Mota
authors: Andrew P
authors: AP
authors: Waters
authors: Chris J
authors: CJ
authors: Janse
authors: Joanne
authors: J
authors: Thompson
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D009033:Culicidae / Q000469:parasitology; D003545:Cysteine / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D056945:Hep G2 Cells; D022781:Hepatocytes / Q000469:parasitology; D006801:Humans; D008018:Life Cycle Stages; D008288:Malaria / Q000469:parasitology* / Q000635:transmission*; D051379:Mice; D008969:Molecular Sequence Data; D033661:Oocysts / Q000737:chemistry / Q000254:growth & development; D010962:Plasmodium berghei / Q000737:chemistry* / Q000235:genetics / Q000254:growth & development* / Q000502:physiology; D015800:Protozoan Proteins / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D016415:Sequence Alignment; D034101:Sporozoites / Q000737:chemistry / Q000254:growth & development
chemical_list: D015800:Protozoan Proteins; D003545:Cysteine