title: Lectin-mediated microfluidic capture and release of leukemic lymphocytes from whole blood.
abstract: Lectins are a group of proteins that bind specifically and reversibly to mono- and oligosaccharide carbohydrate structures that are present on the surfaces of mammalian cells. The use of lectins as capture agents in microfluidic channels was examined with a focus on cells associated with T and B lymphocytic leukemia. In addition to examining the adhesion of Jurkat T and Raji B lymphocytes to a broad panel of lectins, this work also examined the capture of these cells from whole blood. Captured T and B lymphocytes were eluted from the microfluidic devices with a solution of the lectin's inhibiting sugar. The capture and release steps were accomplished in under 1 h. The significance of this work lies within the realm of low-cost capture of abundant target cells with non-stimulatory elution capability.
authors: Department of Chemical Engineering, Northeastern University, 360 Huntington Avenue, Boston, MA 02115, USA.
authors: Dwayne A L
authors: DA
authors: Vickers
authors: Marina
authors: M
authors: Hincapie
authors: William S
authors: WS
authors: Hancock
authors: Shashi K
authors: SK
authors: Murthy
mesh_terms: D001402:B-Lymphocytes / Q000166:cytology* / Q000378:metabolism; D002448:Cell Adhesion; D002469:Cell Separation / Q000295:instrumentation*; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry / Q000378:metabolism; D019169:Jurkat Cells; D007938:Leukemia / Q000097:blood*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D037121:Plant Lectins / Q000737:chemistry / Q000378:metabolism*; D013601:T-Lymphocytes / Q000166:cytology* / Q000378:metabolism
chemical_list: D055767:Immobilized Proteins; D037121:Plant Lectins