title: Export is the default pathway for soluble unfolded polypeptides that accumulate during expression in Escherichia coli.
abstract: Several E. coli endogenous, cytoplasmic proteins that are known clients of the chaperonin GroEL were overexpressed to examine the fate of accumulated unfolded polypeptides. Substantial fractions of about half of the proteins formed insoluble aggregates, consistent with the hypothesis that these proteins were produced at rates or in amounts that exceeded the protein-folding capacity of GroEL. In addition, large fractions of three overexpressed GroEL client proteins were localized in an extra-cytoplasmic, osmotically-sensitive compartment, suggesting they had initially accumulated in the cytoplasm as soluble unfolded polypeptides and thus were able to access a protein export pathway. Consistent with this model, an intrinsically unfoldable, hydrophilic, non-secretory polypeptide was quantitatively exported from the E. coli cytoplasm into an osmotically-sensitive compartment. Our results support the conclusion that a soluble, unfolded conformation alone may be sufficient to direct non-secretory polypeptides into a protein export pathway for signal peptide-independent translocation across the inner membrane, and that export rather than degradation by cytoplasmic proteases is the preferred fate for newly-synthesized, soluble, unfolded polypeptides that accumulate in the cytoplasm. The stable folded conformation of exported GroEL client proteins further suggests that the requirement for GroEL may be conditional on protein folding in the molecularly-crowded environment of the cytoplasm.
authors: Biology Department, Brookhaven National Laboratory, Upton, NY 11973, USA. escottol@notes.cc.sunysb.edu
authors: Elizabeth
authors: E
authors: Scotto-Lavino
authors: Mei
authors: M
authors: Bai
authors: Yian-Biao
authors: YB
authors: Zhang
authors: Paul
authors: P
authors: Freimuth
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D018834:Chaperonin 60 / Q000378:metabolism; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000166:cytology / Q000235:genetics* / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000378:metabolism; D010455:Peptides / Q000032:analysis / Q000235:genetics* / Q000378:metabolism*; D021381:Protein Transport; D058767:Protein Unfolding; D012995:Solubility; D015854:Up-Regulation; D014764:Viral Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism
chemical_list: D018834:Chaperonin 60; D029968:Escherichia coli Proteins; D010455:Peptides; D014764:Viral Proteins