title: Purification of plasmid DNA from Escherichia coli ferments using anion-exchange membrane and hydrophobic chromatography.
abstract: A novel downstream bioprocess was developed to obtain purified plasmid DNA (pDNA) from Escherichia coli ferments. The intermediate recovery and purification of the pDNA in cell lysate was conducted using hollow-fiber tangential filtration and frontal anion-exchange membrane and elution hydrophobic chromatographies. The purity of the solutions of pDNA obtained during each process stage was investigated. The results show that the pDNA solution purity increased 30-fold and more than 99% of RNA in the lysate was removed during the process operations. The combination of membrane operations and hydrophobic interaction chromatography resulted in an efficient way to recover pDNA from cell lysates. A better understanding of membrane-based technology for the purification of pDNA from clarified E. coli lysate was developed in this research.
authors: Departamento de Ingeniería Química y Metalurgia, Universidad de Sonora, Hermosillo, Sonora, México. pguerrero@iq.uson.mx
authors: Patricia
authors: P
authors: Guerrero-Germán
authors: Rosa Ma
authors: RM
authors: Montesinos-Cisneros
authors: D Miguel F
authors: DM
authors: Prazeres
authors: Armando
authors: A
authors: Tejeda-Mansir
mesh_terms: D000837:Anion Exchange Resins / Q000737:chemistry*; D002852:Chromatography, Ion Exchange / Q000295:instrumentation / Q000379:methods*; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004926:Escherichia coli / Q000378:metabolism*; D005285:Fermentation; D005374:Filtration / Q000295:instrumentation / Q000379:methods; D057927:Hydrophobic and Hydrophilic Interactions; D010957:Plasmids / Q000302:isolation & purification*; D012329:RNA, Bacterial / Q000302:isolation & purification
chemical_list: D000837:Anion Exchange Resins; D004269:DNA, Bacterial; D012329:RNA, Bacterial