title: Application of sulphanilamides disazo dyes with Tropaeolin O for simple, rapid and sensitive spectrophotometric assay of medicines.
abstract: A rapid, simple and sensitive spectrophotometric method for the determination of some sulphanilamides is described. The method is based on the formation of blue coloured disazo dyes by the diazotization of sulphonamides viz. sulphanilamide (SA), sulphamerazine (SMR), sulphamethazine (SMZ), sulphadimethoxine (SDM), sulphamethoxazole (SMX), sulphadiazine (SDA), sulfathiazole (STZ), sulphaguanidine (SGN), sulphamonomethoxine (SMM), sulphamethoxypyridazine (SMP) in 0.5M hydrochloric acid media at ice bath followed by the azocoupling reaction with acid monoazo dye Tropaeolin O (TrO) at pH=10.5. Formed products are stable for 10h at room temperature. Effective molar absorptivities at absorbance maxima 595nm for disazo dyes were ∼10(4)M(-1)cm(-1). Stoichiometric ratios of the components of disazo dyes were determined by means of mole ratio and continuous variations methods. Linear ranges for sulphanilamides determination were 0.4-14.0μgml(-1). The methods were successfully approved at suphanilamides determination in model solutions and commercial pharmaceutical preparations.
authors: Ivan Franko Lviv National University, Chemistry Faculty, Analytical Chemistry Department, Kyrylо & Mefodiy Str., 6, 79005 Lviv, Ukraine. boiko maria@ukr.net
authors: Maria
authors: M
authors: Boiko
authors: Teodoziya
authors: T
authors: Vrublevska
authors: Olha
authors: O
authors: Korkuna
authors: Grigory
authors: G
authors: Teslyar
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis*; D001391:Azo Compounds / Q000737:chemistry*; D001557:Benzenesulfonates / Q000737:chemistry*; D004396:Coloring Agents / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013053:Spectrophotometry / Q000191:economics / Q000379:methods*; D000077145:Sulfanilamide; D013424:Sulfanilamides / Q000032:analysis*; D013607:Tablets / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D001391:Azo Compounds; D001557:Benzenesulfonates; D004396:Coloring Agents; D013424:Sulfanilamides; D013607:Tablets; D000077145:Sulfanilamide; C014722:2-naphthol orange