title: Profiling of influenza viruses by high-throughput carbohydrate membrane array.
abstract: BACKGROUND
  Carbohydrate-protein interactions participate in many biological functions. To characterize the binding interactions represents a longstanding challenge.
  
  
  METHODS
  We developed a glycan membrane array to study the interactions of carbohydrates with lectins, proteins and viruses, including 17 lectins, four antibodies (that are specific to different Lewis antigens), one recombinant H5N1 hemagglutinin, and five influenza B clinical isolates.
  
  
  RESULTS
  The results were found to be comparable to previous reports, indicating the efficacy and reliability of our developed method.
  
  
  CONCLUSIONS
  This carbohydrate membrane array represents a convenient, reliable and low-cost method to examine the carbohydrate-binding features of various proteins, high-throughput drug screening and the glycan-binding surveillance of influenza viruses.
authors: Department of Medical Laboratory Science and Biotechnology, National Cheng Kung University, No. 1, University Road, Tainan 70101, Taiwan.
authors: Weng-I
authors: WI
authors: Lao
authors: Ya-Fang
authors: YF
authors: Wang
authors: Yu-Dai
authors: YD
authors: Kuo
authors: Chun-Hung
authors: CH
authors: Lin
authors: Tsung-Chain
authors: TC
authors: Chang
authors: Ih-Jen
authors: IJ
authors: Su
authors: Jen-Ren
authors: JR
authors: Wang
authors: Chuan-Fa
authors: CF
authors: Chang
mesh_terms: D000906:Antibodies / Q000737:chemistry / Q000378:metabolism; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000737:chemistry* / Q000378:metabolism; D009981:Influenza B virus / Q000378:metabolism*; D037102:Lectins / Q000737:chemistry / Q000378:metabolism; D008566:Membranes / Q000378:metabolism; D046228:Microarray Analysis / Q000379:methods*; D011134:Polysaccharides / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry / Q000378:metabolism
chemical_list: D000906:Antibodies; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D037102:Lectins; D011134:Polysaccharides; D011506:Proteins