title: Two-step purification and in vitro characterization of a hemolysin from the venom of jellyfish Cyanea nozakii Kishinouye.
abstract: Hemolysin is one of the most hazardous components in the venom of Cyanea nozakii Kishinouye. Here we describe the purification and in vitro characterization of the hemolysin, which we named CnPH. The CnPH was isolated by anion-exchange and size-exclusion chromatography from the nematocyst venom. Two protein bands with molecular masses of 20 kDa, 60 kDa respectively were shown in the reducing SDS-PAGE analysis of the CnPH. And Approximately 5 μg/mL of the CnPH resulted in 50% hemolysis of the erythrocyte suspension. The hemolytic activity of the CnPH was both temperature and pH dependent. Moreover, it was significantly inhibited in the presence of divalent metal cations, including Cu(2+), Mg(2+), Mn(2+), Zn(2+) and Ca(2+), but enhanced in the presence of EDTA. However, how CnPH performs its hemolytic activity is not yet clear, therefore the mechanism of the hemolytic activity of the CnPH is under research.
authors: Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, China. lirongfeng07@qdio.ac.cn
authors: Rongfeng
authors: R
authors: Li
authors: Huahua
authors: H
authors: Yu
authors: Jinhua
authors: J
authors: Feng
authors: Ronge
authors: R
authors: Xing
authors: Song
authors: S
authors: Liu
authors: Lin
authors: L
authors: Wang
authors: Yukun
authors: Y
authors: Qin
authors: Kecheng
authors: K
authors: Li
authors: Pengcheng
authors: P
authors: Li
mesh_terms: D000818:Animals; D002681:China; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003064:Cnidarian Venoms / Q000737:chemistry*; D004492:Edetic Acid / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D006460:Hemolysin Proteins / Q000819:agonists / Q000037:antagonists & inhibitors / Q000302:isolation & purification* / Q000494:pharmacology*; D006461:Hemolysis / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D019216:Metals, Heavy / Q000494:pharmacology; D009792:Oceans and Seas; D037401:Scyphozoa / Q000737:chemistry*; D013696:Temperature
chemical_list: D003064:Cnidarian Venoms; D006460:Hemolysin Proteins; D019216:Metals, Heavy; D004492:Edetic Acid