title: Akt/mammalian target of rapamycin signaling pathway regulates neurite outgrowth in cerebellar granule neurons stimulated by methylcobalamin.
abstract: Methylcobalamin (MeCbl), a vitamin B12 analog, promotes neurite outgrowth by activating Akt in neurons. However, Akt is involved in many cellular functions, and the downstream signal of Akt that promotes neurite outgrowth in neurons in the presence of MeCbl remains obscure. Mammalian target of rapamycin (mTOR) is a serine/threonine protein kinase that regulates multiple cellular functions including neurite outgrowth. mTOR is regarded as important for the regeneration of injured nerves. In this study, we examined the relationship between MeCbl and mTOR activity and found that MeCbl increases mTOR activity via the activation of Akt and promotes neurite outgrowth in cerebellar granule neurons via the activation of mTOR.
authors: Department of Orthopaedics, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan. kokada2433@snow.ocn.ne.jp
authors: Kiyoshi
authors: K
authors: Okada
authors: Hiroyuki
authors: H
authors: Tanaka
authors: Ko
authors: K
authors: Temporin
authors: Michio
authors: M
authors: Okamoto
authors: Yusuke
authors: Y
authors: Kuroda
authors: Hisao
authors: H
authors: Moritomo
authors: Tsuyoshi
authors: T
authors: Murase
authors: Hideki
authors: H
authors: Yoshikawa
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology*; D002867:Chromones / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D009025:Morpholines / Q000494:pharmacology; D016501:Neurites / Q000187:drug effects*; D009474:Neurons / Q000166:cytology* / Q000187:drug effects; D051058:Oncogene Protein v-akt / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000187:drug effects*; D020123:Sirolimus / Q000494:pharmacology; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism*; D014805:Vitamin B 12 / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D002867:Chromones; D004791:Enzyme Inhibitors; D009025:Morpholines; C085911:2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one; C019476:mecobalamin; C546845:mTOR protein, rat; D051058:Oncogene Protein v-akt; D058570:TOR Serine-Threonine Kinases; D014805:Vitamin B 12; D020123:Sirolimus