title: BacMam-mediated gene delivery into multipotent mesenchymal stromal cells.
abstract: Baculoviruses have been used over the last several decades for high-level protein production in insect cells. Recently, modified baculovirus containing a mammalian promoter, known as BacMam virus, has been shown to give high transduction efficiencies across several cell types with minimal cytopathic effects. Cell types amenable to BacMam transduction include primary and adult stem cells. The shuttle vectors used in the construction of BacMam viruses can hold gene fragments up to 38 kb in size, and multiple BacMam viruses can be used in a single transduction for the delivery of more than one gene. BacMam technology has been used in the delivery and expression of targeted fluorescent protein cellular markers, small interfering RNAi, and extensively in the development of cell-based assays. BacMam offers an ideal method for the delivery and expression of large genes in hard-to-transfect cells such as primary and adult stem cells. In this chapter, we describe methods of generating high titer stocks of BacMam for transducing MSC and their derivatives.
authors: Primary and Stem Cell Systems, Life Technologies, Carlsbad, CA, USA.
authors: Michael
authors: M
authors: O'Grady
authors: Robert H
authors: RH
authors: Batchelor
authors: Kelly
authors: K
authors: Scheyhing
authors: Christopher W
authors: CW
authors: Kemp
authors: George T
authors: GT
authors: Hanson
authors: Uma
authors: U
authors: Lakshmipathy
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D018929:Cell Culture Techniques; D015925:Cryopreservation; D004274:DNA, Recombinant / Q000235:genetics / Q000302:isolation & purification; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D005818:Genetic Engineering; D007313:Insecta / Q000166:cytology; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D039902:Multipotent Stem Cells / Q000166:cytology / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics*; D013535:Suspensions; D014162:Transfection / Q000379:methods*
chemical_list: D004274:DNA, Recombinant; D004279:DNA, Viral; D013535:Suspensions