title: Inhibition of nitric oxide production in lipopolysaccharide-stimulated RAW264.7 macrophage cells by lignans isolated from Euonymus alatus leaves and twigs.
abstract: The 80% methanolic extract of Euonymus alatus leaves and twigs afforded three new lignans, (-)-threo-4,9,4',9'-tetrahydroxy-3,7,3',5'-tetramethoxy-8-O-8'-neolignan (1), (-)-threo-4,9,4',9'-tetrahydroxy-3,5,7,3'-tetramethoxy-8-O-8'-neolignan (2), (7R,8R,7'R)-(+)-lyoniresinol (3), together with seventeen known lignans (4-20). The structures of 1-20 were elucidated by extensive 1D and 2D spectroscopic methods including (1)H NMR, (13)C NMR, (1)H-(1)H COSY, HMQC, HMBC and NOESY. All the isolated compounds except for dilignans (19 and 20) significantly inhibited nitric oxide production in lipopolysaccharide-stimulated RAW264.7 cells.
authors: College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University, Seoul 151-742, Republic of Korea.
authors: Eun Ju
authors: EJ
authors: Jeong
authors: Jung Hee
authors: JH
authors: Cho
authors: Sang Hyun
authors: SH
authors: Sung
authors: Sun Yeou
authors: SY
authors: Kim
authors: Young Choong
authors: YC
authors: Kim
mesh_terms: D000818:Animals; D000840:Anisoles / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D002460:Cell Line; D029756:Euonymus / Q000737:chemistry*; D017705:Lignans / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000633:toxicity; D008264:Macrophages / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008968:Molecular Conformation; D009281:Naphthalenes / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D009569:Nitric Oxide / Q000378:metabolism*; D018515:Plant Leaves / Q000737:chemistry
chemical_list: D000840:Anisoles; D017705:Lignans; D008070:Lipopolysaccharides; D009281:Naphthalenes; C502379:lyoniresinol; C559405:threo-4,9,4',9'-tetrahydroxy-3,5,7,3'-tetramethoxy-8-O-8'-neolignan; C559406:threo-4,9,4',9'-tetrahydroxy-3,7,3',5'-tetramethoxy-8-O-8'-neolignan; D009569:Nitric Oxide