title: Optimization of hyperthermia and dendritic cell immunotherapy for squamous cell carcinoma.
abstract: The aims of this study were to explore the feasibility of a novel combination therapy comprising hyperthermia (HT) and dendritic cell (DC) application for squamous cell carcinoma (SCC), and to optimize the conditions of this therapy. In vitro, the correlation between maturation of DCs by co-culture with SCCVII cells and HT was investigated. DCs did not mature in simple HT (43 °C for 30 min) with SCCVII cells. On the other hand, DC maturation occurred in additional mild HT (mHT: 41 °C for 30 min) with simple HT. To assess whether additional mHT was effective, in vivo combined treatment was performed using tumor-bearing C3H/HeJ mice. A more suppressive effect of tumor growth was observed, and cytotoxic T cell infiltration was significantly increased by adding mHT compared to conventional only simple HT with DCs. These phenomena also occurred in non-treated contralateral tumors as well as treated ones. Our data suggest that the combination of 43 °C preheated simple HT SCCVII tumors and additional 41 °C heat mHT promotes DC maturation, resulting in suppression of tumor growth systemically and lifetime prolongation in mice. A three-step process of additional mHT after local HT and intratumoral immature DC (iDC) injection could be a more effective and novel method for the treatment of SCC.
authors: Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors: Koushi
authors: K
authors: Matsumoto
authors: Noriyuki
authors: N
authors: Yamamoto
authors: Sumitaka
authors: S
authors: Hagiwara
authors: Masaki
authors: M
authors: Saito
authors: Hiroki
authors: H
authors: Furue
authors: Toshio
authors: T
authors: Shigetomi
authors: Yuji
authors: Y
authors: Narita
authors: Kenji
authors: K
authors: Mitsudo
authors: Iwai
authors: I
authors: Tohnai
authors: Takeshi
authors: T
authors: Kobayashi
authors: Minoru
authors: M
authors: Ueda
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000628:therapy*; D003713:Dendritic Cells / Q000637:transplantation*; D005260:Female; D005455:Fluorescent Antibody Technique; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis; D006979:Hyperthermia, Induced; D007167:Immunotherapy / Q000379:methods*; D051379:Mice; D008809:Mice, Inbred C3H
chemical_list: D018840:HSP70 Heat-Shock Proteins