title: Poliovirus serotype-specific VP1 sequencing primers.
abstract: The Global Polio Laboratory Network routinely uses poliovirus-specific PCR primers and probes to determine the serotype and genotype of poliovirus isolates obtained as part of global poliovirus surveillance. To provide detailed molecular epidemiologic information, poliovirus isolates are further characterized by sequencing the ~900-nucleotide region encoding the major capsid protein, VP1. It is difficult to obtain quality sequence information when clinical or environmental samples contain poliovirus mixtures. As an alternative to conventional methods for resolving poliovirus mixtures, sets of serotype-specific primers were developed for amplifying and sequencing the VP1 regions of individual components of mixed populations of vaccine-vaccine, vaccine-wild, and wild-wild polioviruses.
authors: Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA. DKilpatrick@cdc.gov
authors: David R
authors: DR
authors: Kilpatrick
authors: Jane C
authors: JC
authors: Iber
authors: Qi
authors: Q
authors: Chen
authors: Karen
authors: K
authors: Ching
authors: Su-Ju
authors: SJ
authors: Yang
authors: Lina
authors: L
authors: De
authors: Mark D
authors: MD
authors: Mandelbaum
authors: Brian
authors: B
authors: Emery
authors: Ray
authors: R
authors: Campagnoli
authors: Cara C
authors: CC
authors: Burns
authors: Olen
authors: O
authors: Kew
mesh_terms: D036022:Capsid Proteins / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics*; D006801:Humans; D017955:Poliovirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D014773:Virology / Q000379:methods
chemical_list: D036022:Capsid Proteins; D017931:DNA Primers; C442540:VP1 protein, Poliovirus