title: N-terminal region of the PB1-F2 protein is responsible for increased expression of influenza A viral protein PB1.
abstract: Influenza A virus (IAV) PB1-F2 protein is encoded by an alternative reading frame (+1) within the PB1 gene. PB1-F2 has been shown to contribute to the pathogenesis of influenza virus infection as well as to the secondary bacterial infection. More recently has been shown that PB1-F2 protein may regulate a viral RNA (vRNA) polymerase activity by the interaction with PB1 protein. We proved that PB1-F2 protein increased the level of expression of PB1 protein and vRNA in the infected cells. Moreover, we demonstrated that a higher level of vRNA expression resulted in the increase of expression of multiple viral proteins, including NP, M1, and NS1. Finally, we used plasmids expressing N-terminal (1-50 aa) or C-terminal (51-87 aa) region of the PB1-F2 molecule for transfection of MDCK cells co-infected with influenza A/Puerto Rico/8/34 (H1N1) virus deficient in the PB1-F2 protein expression (PR8ΔPB1-F2). These experiments clearly showed that N-terminal region of PB1-F2 protein was responsible for the increase in PB1 protein expression. C-terminal region of PB1-F2 protein had no effect. Thus, we have identified the important function for N-terminal region of PB1-F2 protein.
authors: Slovak Academy of Sciences, Bratislava, Slovak Republic.
authors: I
authors: I
authors: Košík
authors: I
authors: I
authors: Krejnusová
authors: M
authors: M
authors: Bystrická
authors: K
authors: K
authors: Poláková
authors: G
authors: G
authors: Russ
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004285:Dogs; D005260:Female; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D011647:Puerto Rico; D012367:RNA, Viral / Q000235:genetics; D016364:Reading Frames; D019444:Vaccines, DNA / Q000235:genetics; D014764:Viral Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism*
chemical_list: C442816:PB1-F2 protein, Influenza A virus; D012367:RNA, Viral; D019444:Vaccines, DNA; D014764:Viral Proteins; C077346:influenza virus polymerase basic protein 1; D012321:DNA-Directed RNA Polymerases