title: Dual-functional composite with anticoagulant and antibacterial properties based on heparinized silk fibroin and chitosan.
abstract: Heparinized biomaterials exhibit great anticoagulant properties. However, they promote proliferation of Staphylococcus aureus (S. aureus) and therefore cause infection within the bloodstream upon implantation in vivo. In the present study, an interesting dual-functional composite with anticoagulant and antibacterial properties based on heparinized silk fibroin and chitosan was synthesized. First, heparin was grafted onto the silk fibroin by covalent immobilization with N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide (EDC) and N-hydroxysuccinimide (NHS). All data gathered from Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and elemental analysis (EA) indicated that the heparin was successfully immobilized onto the silk fibroin. The dual-functional composite of heparinized silk fibroin and chitosan was then fabricated by a blending method. The anticoagulant activity of the heparinized materials was evaluated using the prothrombin time (PT), activated partial thromboplastin time (APTT) and thrombin time (TT). The results showed that both heparinized silk fibroin and the composite material exhibited better hemocompatibility in comparison with single silk fibroin or chitosan. The antibacterial property of the materials was investigated by the pour-plate method. Results further suggested that the composite antibacterial property with respect to S. aureus was significantly enhanced. The dual-functionality of the composite material may supply a potential choice in blood contact devices.
authors: Advanced Biomaterials and Tissue Engineering Center, Huazhong University of Science and Technology, Life Science Building, 1037 Luoyu Road, Wuhan, 430074, People's Republic of China.
authors: Jianglin
authors: J
authors: Wang
authors: Wei
authors: W
authors: Hu
authors: Qun
authors: Q
authors: Liu
authors: Shengmin
authors: S
authors: Zhang
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D000925:Anticoagulants / Q000737:chemistry* / Q000494:pharmacology; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology; D005349:Fibroins / Q000737:chemistry* / Q000494:pharmacology / Q000648:ultrastructure; D006493:Heparin / Q000737:chemistry* / Q000494:pharmacology; D008855:Microscopy, Electron, Scanning; D008956:Models, Chemical; D015394:Molecular Structure; D010314:Partial Thromboplastin Time; D011517:Prothrombin Time; D047011:Silk / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development; D013918:Thrombin Time
chemical_list: D000900:Anti-Bacterial Agents; D000925:Anticoagulants; D001672:Biocompatible Materials; D047011:Silk; D006493:Heparin; D005349:Fibroins; D048271:Chitosan