title: Long term persistence of IgE anti-influenza virus antibodies in pediatric and adult serum post vaccination with influenza virus vaccine.
abstract: The production of IgE specific to different viruses (HIV-1, Parvovirus B19, Parainfluenza virus, Varicella Zoster Virus), and the ability of IgE anti-HIV-1 to suppress HIV-1 production in vitro, strongly suggest an important role for IgE and/or anti viral specific IgE in viral pathogenesis. Nevertheless, the presence and persistence of IgE anti-Influenza virus antibodies has not been studied. Total serum IgE and specific IgE and IgG anti-Influenza virus antibodies were studied in children (N = 3) (m/f 14-16 y/o) and adults (N = 3) (m/f, 41-49 y/o) 2-20 months after vaccination with Influenza virus (Flumist(®) or Fluzone(®)), as well as in non-vaccinated children (N = 2). (UniCAP total IgE Fluoroenzymeimmunoassay, ELISA, Immunoblot). We found that serum of vaccinated children and adults contained IgE and IgG anti-Influenza virus antibodies approaching two years post vaccination. Non-vaccinated children did not make either IgE or IgG anti-Influenza antibodies. Similar levels of IL-2, IFN-γ, IL-4, and IL-10 cytokines were detected in serum of vaccinated compared with non vaccinated subjects (p > 0.05), as well as between vaccinated adults compared with vaccinated children and non vaccinated subjects (p > 0.05). Vaccinated children and adults continue to produce IgE anti-Influenza virus antibodies long term post vaccination. The long term production of IgE anti-Influenza virus antibodies induced by vaccination may contribute to protective immunity against Influenza.
authors: Department of Pediatrics, SUNY Downstate Medical Center, Brooklyn, New York 11203, USA. tamar.smith-norowitz@downstate.edu
authors: Tamar A
authors: TA
authors: Smith-Norowitz
authors: Darrin
authors: D
authors: Wong
authors: Melanie
authors: M
authors: Kusonruksa
authors: Kevin B
authors: KB
authors: Norowitz
authors: Rauno
authors: R
authors: Joks
authors: Helen G
authors: HG
authors: Durkin
authors: Martin H
authors: MH
authors: Bluth
mesh_terms: D000293:Adolescent; D000328:Adult; D005260:Female; D006801:Humans; D056724:Immunity, Humoral / Q000276:immunology*; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology*; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007252:Influenza Vaccines / Q000276:immunology*; D007371:Interferon-gamma / Q000097:blood; D007378:Interleukins / Q000097:blood; D008297:Male; D008875:Middle Aged; D009975:Orthomyxoviridae / Q000276:immunology*; D013997:Time Factors; D014611:Vaccination / Q000379:methods
chemical_list: D007074:Immunoglobulin G; D007252:Influenza Vaccines; D007378:Interleukins; D007073:Immunoglobulin E; D007371:Interferon-gamma