title: Towards a synthetic avidin mimic.
abstract: A series of streptavidin-mimicking molecularly imprinted polymers has been developed and evaluated for their biotin binding characteristics. A combination of molecular dynamics and NMR spectroscopy was used to examine potential polymer systems, in particular with the functional monomers methacrylic acid and 2-acrylamidopyridine. The synthesis of copolymers of ethylene dimethacrylate and one or both of these functional monomers was performed. A combination of radioligand binding studies and surface area analyses demonstrated the presence of selectivity in polymers prepared using methacrylic acid as the functional monomer. This was predicted by the molecular dynamics studies showing the power of this methodology as a prognostic tool for predicting the behavior of molecularly imprinted polymers.
authors: Bioorganic & Biophysical Chemistry Laboratory, Section for Biomaterials and Medicinal Chemistry, School of Natural Sciences, Linnaeus University, 39182 Kalmar, Sweden. jesper.wiklander@lnu.se
authors: Jesper
authors: J
authors: Wiklander
authors: Björn C G
authors: BC
authors: Karlsson
authors: Teodor
authors: T
authors: Aastrup
authors: Ian A
authors: IA
authors: Nicholls
mesh_terms: D001665:Binding Sites; D001710:Biotin / Q000378:metabolism*; D008689:Methacrylates / Q000737:chemistry; D056004:Molecular Dynamics Simulation; D054802:Molecular Imprinting / Q000379:methods; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism; D019809:Streptavidin / Q000737:chemistry*
chemical_list: D008689:Methacrylates; D011108:Polymers; C008384:methacrylic acid; D001710:Biotin; D019809:Streptavidin