title: Mimicry technology: suppressing small RNA activity in plants.
abstract: Small RNA suppression constitutes one of the major difficulties for a full molecular characterization of their specific roles in plants. Taking advantage of the latest insights into the new post-biogenesis layer of regulation in microRNA (miRNA) activity, it is possible to overcome the above-mentioned limitation (Nat Genet 39:1033-1037, 2007). We engineered the IPS1 non-coding RNA to bear a complementary sequence to a given miRNA family, resulting in specific sequestration of RISC complexes. MIMIC technology allows for the constitutive release of all of the potential targets of a miRNA family as well as tissue-specific and inducible suppression of its activity.
authors: Max Planck Institute for Developmental Biology, Tübingen, Germany. ignacio.rubio@tuebingen.mpg.de
authors: Ignacio
authors: I
authors: Rubio-Somoza
authors: Pablo Andrés
authors: PA
authors: Manavella
mesh_terms: D005818:Genetic Engineering / Q000379:methods*; D035683:MicroRNAs / Q000235:genetics*; D018716:Molecular Mimicry; D010944:Plants / Q000235:genetics*; D011401:Promoter Regions, Genetic; D018749:RNA, Plant / Q000235:genetics; D034743:RNA-Induced Silencing Complex / Q000235:genetics
chemical_list: D035683:MicroRNAs; D018749:RNA, Plant; D034743:RNA-Induced Silencing Complex