title: Crystallization and preliminary X-ray crystallographic analysis of CTX-M-15, an extended-spectrum β-lactamase conferring worldwide emerging antibiotic resistance.
abstract: CTX-M-15, an extended-spectrum β-lactamase emerging worldwide, hydrolyzes lactam ring of β-lactam antibiotics, and thus causes therapeutic failure and a lack of eradication of pathogenic bacteria by third-generation β-lactams. Therefore, the enzyme is a potential target for developing agents against pathogens isolated from patients suffering from nosocomial infections. The CTX-M-15 protein was purified and crystallized at 298 K. X-ray diffraction data from CTX-M-15 crystal have been collected to 1.46 Å resolution using synchrotron radiation. The crystal of CTX-M-15 belongs to space group P2(1)2(1)2(1), with unit-cell parameters a = 45.50, b = 44.23, and c = 116.92 Å. Analysis of the packing density shows that the asymmetric unit probably contains two molecules with a solvent content of 41.26%.
authors: Drug Resistance Proteomics Laboratory, Department of Biological Sciences, Myongji University, Yongin, Gyeonggido 449-728, Republic of Korea.
authors: Young Jun
authors: YJ
authors: An
authors: Jung Hun
authors: JH
authors: Lee
authors: Ha Il
authors: HI
authors: Jung
authors: Seung Ghyu
authors: SG
authors: Sohn
authors: Jae Jin
authors: JJ
authors: Lee
authors: Kwang Seung
authors: KS
authors: Park
authors: Xing
authors: X
authors: Wu
authors: Byeong Chul
authors: BC
authors: Jeong
authors: Choong-Min
authors: CM
authors: Kang
authors: Sun-Shin
authors: SS
authors: Cha
authors: Sang Hee
authors: SH
authors: Lee
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000737:chemistry / Q000201:enzymology* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000188:drug therapy / Q000201:enzymology*; D006801:Humans; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: C473109:beta-lactamase CTX-M-15; D001618:beta-Lactamases