title: Effect of C-xyloside on morphogenesis of the dermal epidermal junction in aged female skin. An ultrastuctural pilot study.
abstract: A placebo-controlled randomized pilot study was performed on five postmenopausal women aged from 60 to 75 years. The women applied 320 mg (2 mg/cm(2)) of either placebo or 10% C-β-D-xylopyranoside-2-hydroxy-propane (C-xyloside) cream to each outer forearm twice daily for 3 months. At the end of the treatment, skin biopsies were collected from application areas on both forearms. Transmission electron microscope examinations revealed skin ultrastructural changes at the dermal epidermal junction (DEJ) after 10% C-xyloside application for 3 months. The morphological appearance of the DEJ showed strong improvements, with more homogeneous and regular lamina densa in the C-xyloside-treated compared to the placebo treated skin areas. The number of zones showing basement membrane re-duplication was indeed strikingly reduced on C-xyloside-treated skin. These ultrastructural results were further confirmed by a statistically significant increase in the expression levels of α6-integrin the and laminin-332, as estimated by immunohistochemistry. Altogether, these data suggest that topical C-xyloside application in vivo may be efficient in inducing a better dermal-epidermal cohesion when such a junction is deficient, as is the case in photo-aged or chronologically aged skin. Moreover, a statistically significant increase in CD44 expression was noted in the epidermis of C-xyloside-treated compared to the placebo treated skin areas.
authors: L'Oréal Recherche, 188, rue Paul Hochart, BP 553, 94152 Chevilly-Larue. cdeloche@rd.loreal.com
authors: Claire
authors: C
authors: Deloche
authors: Anne Marie
authors: AM
authors: Minondo
authors: Bruno A
authors: BA
authors: Bernard
authors: Françoise
authors: F
authors: Bernerd
authors: Fabien
authors: F
authors: Salas
authors: Julien
authors: J
authors: Garnier
authors: Emmanuelle
authors: E
authors: Tancrède
mesh_terms: D000368:Aged; D015815:Cell Adhesion Molecules / Q000378:metabolism; D020405:Dermis / Q000187:drug effects / Q000254:growth & development* / Q000648:ultrastructure*; D004311:Double-Blind Method; D004817:Epidermis / Q000187:drug effects / Q000254:growth & development* / Q000648:ultrastructure*; D005260:Female; D006027:Glycosides / Q000494:pharmacology*; D006801:Humans; D007150:Immunohistochemistry; D039503:Integrin alpha6 / Q000378:metabolism; D008875:Middle Aged; D009024:Morphogenesis / Q000187:drug effects*; D010865:Pilot Projects; D015595:Skin Aging / Q000187:drug effects / Q000473:pathology*; D000097794:Kalinin
chemical_list: D015815:Cell Adhesion Molecules; D006027:Glycosides; D039503:Integrin alpha6; D000097794:Kalinin; C531898:C-xylopyranoside-2-hydroxypropane