title: Molecular analysis and characterization of a urease gene operon from Campylobacter sputorum biovar paraureolyticus.
abstract: When recombinant plasmid DNA from a genomic DNA library and inverse PCR products of Campylobacter sputorum biovar paraureolyticus LMG17591 strain were analyzed, an approximate 6.5-kb pair region, encoding a urease gene operon, was identified. Within the operon, seven closely spaced and putative open reading frames for ureG, ureH(D), ureA, ureB, ureC, ureE, and ureF were detected in order. A possible overlap was detected between ureG and ureH(D), ureH(D) and ureA, and ureE and ureF. In addition, two putative promoter structures, probable ribosome-binding sites and a putative ρ-independent transcriptional terminator structure were identified. The urease gene operon transcription in the cells was confirmed by the reverse transcription-PCR analysis. A neighbor-joining tree constructed based on the nucleotide sequence information of urease genes showed that C. sputorum biovar paraureolyticus formed a cluster with Arcobacter butzleri, urease-positive thermophilic Campylobacter and some Helicobacter spp., separating those from the other urease-producing bacteria, suggesting a commonly shared ancestry among these organisms.
authors: Graduate School of Environmental Health Sciences, Azabu University, 1-17-71, Fuchinobe, Chuo-ku Sagamihara, 252-5201, Japan.
authors: Y
authors: Y
authors: Kakinuma
authors: K
authors: K
authors: Hayashi
authors: A
authors: A
authors: Tazumi
authors: J
authors: J
authors: Hirayama
authors: J E
authors: JE
authors: Moore
authors: B C
authors: BC
authors: Millar
authors: T
authors: T
authors: Kuribayashi
authors: M
authors: M
authors: Matsuda
mesh_terms: D000818:Animals; D044884:Campylobacter sputorum / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification; D002417:Cattle; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D020869:Gene Expression Profiling; D015723:Gene Library; D023061:Gene Order; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D009876:Operon; D010802:Phylogeny; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013728:Terminator Regions, Genetic; D014510:Urease / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D014510:Urease