title: A novel porcine gene--SLC9A3R2, differentially expressed in the longissimus muscle tissues from Meishan and Large White pigs.
abstract: The mRNA differential display technique was performed to investigate the differences of gene expression in the longissimus muscle tissues from Meishan and Large White pigs. One novel gene that was differentially expressed was identified through semi-quantitative RT-PCR and the cDNA complete sequence was then obtained using the rapid amplification of cDNA ends (RACE) method. The nucleotide sequence of the gene is not homologous to any of the known porcine genes. The sequence prediction analysis revealed that the open reading frame of this gene encodes a protein of 337 amino acids that has high homology with the solute carrier family 9 isoform 3 regulator 2 isoform a of four species--human (92 %), mouse (91 %), rat (90 %), rabbit (87 %)--so that this gene can be defined as swine SLC9A3R2 gene. The phylogenetic tree analysis revealed that the swine SLC9A3R2 gene has the closest genetic relationship with the SLC9A3R2 gene of human. This gene is structured in seven exons and six introns as revealed by computer-assisted analysis. The tissue expression analysis indicated that the swine SLC9A3R2 gene is differentially expressed in different tissues. Our experiment is the first to establish the primary foundation for further research on the swine SLC9A3R2 gene.
authors: Chongqing Key Laboratory of Pig Industry Sciences, Chongqing Academy of Animal Sciences, Rongchang, China.
authors: Z
authors: Z
authors: Jiugang
authors: L
authors: L
authors: Jing
authors: L
authors: L
authors: Yonggang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003433:Crosses, Genetic; D005091:Exons / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D007438:Introns / Q000235:genetics; D008297:Male; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000378:metabolism*; D021141:Nucleic Acid Amplification Techniques; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D034421:Sus scrofa / Q000235:genetics* / Q000378:metabolism