title: Experimental inoculation of raccoons (Procyon lotor) with Spiroplasma mirum and transmissible mink encephalopathy (TME).
abstract: The primary objective of this study was to determine whether or not Spiroplasma mirum would be capable of producing lesions of transmissible spongiform encephalopathy (TSE) when inoculated in raccoons (Procyon lotor) and, if that was possible, to compare the clinicopathological findings with those of transmissible mink encephalopathy (TME) in the same experimental model. For this purpose, 5 groups (n = 5) of raccoon kits were inoculated intracerebrally with either S. mirum and/or TME. Two other groups (n = 5) of raccoon kits served as sham-inoculated controls. All animals inoculated with TME, either alone or in combination, showed clinical signs of neurologic disorder and were euthanized within 6 mo post-inoculation (MPI). None of the carcasses revealed gross lesions. Spongiform encephalopathy was observed by light microscopy and the presence of abnormal disease-causing prion protein (PrP(d)) was detected by immunohistochemistry (IHC) and Western blot (WB) techniques in only the raccoons administered TME. Raccoons inoculated with Spiroplasma, but not administered TME agent, were euthanized at 30 MPI. They did not show clinical neurologic signs, their brains did not have lesions of spongiform encephalopathy, and their tissues were negative for S. mirum by polymerase chain reaction (PCR) and for PrP(d) by IHC and WB techniques. The results of this study indicate that Spiroplasma mirum does not induce TSE-like disease in raccoons.
authors: National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Ames, Iowa 50010, USA. ahamir@mdanderson.org
authors: Amir N
authors: AN
authors: Hamir
authors: Justin J
authors: JJ
authors: Greenlee
authors: Thad B
authors: TB
authors: Stanton
authors: Jodi D
authors: JD
authors: Smith
authors: Stephanie
authors: S
authors: Doucette
authors: Robert A
authors: RA
authors: Kunkle
authors: Judith A
authors: JA
authors: Stasko
authors: Juergen A
authors: JA
authors: Richt
authors: Marcus E
authors: ME
authors: Kehrli
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D017096:Prion Diseases / Q000382:microbiology / Q000473:pathology / Q000503:physiopathology / Q000662:veterinary*; D011328:Prions / Q000032:analysis; D011821:Raccoons / Q000382:microbiology*; D011897:Random Allocation; D013149:Spiroplasma / Q000472:pathogenicity*
chemical_list: D011328:Prions