title: Alternative bioassay for the detection of saxitoxin using the speckled cockroach (Nauphoeta cinerea).
abstract: Paralytic shellfish poisoning (PSP) toxins produced by cyanobacteria pose a risk to public health as they occur in drinking water reservoirs and recreational lakes and accumulate in the food chain. One of these PSP toxins, saxitoxin (STX) is one of the most toxic nonprotein substances known. Accordingly, there is a requirement to monitor for these toxins. The standard bioassay used to detect these toxins is the mouse bioassay; however, its use is constrained by animal ethics guidelines and practical considerations. Reported here is the use of the globally distributed speckled cockroach Nauphoeta cinerea as a bioassay test organism for the selective detection of PSP toxicity of Anabaena circinalis aqueous extract and STX. N. cinerea was shown to be tolerant to pure cylindrospermopsin (CYN) and microcystin-LR (MC-LR) at doses 10-fold greater than mouse LD₅₀ values while being sensitive to STX. Similarly, N. cinerea was shown to be tolerant of toxin-containing aqueous extracts of Cylindrospermopsis raciborskii, Microcystis aeruginosa, and Nodularia spumigena while being sensitive to A. circinalis. Peak sensitivity to STX was 60 min postinjection with a KD₅₀ of 31.2 ng/g body weight. While this was approximately 3-fold less sensitive than the mouse bioassay, the insect test organism was around 34-fold smaller in mass than a mouse (20 g); thus one-tenth the amount of toxin in absolute quantity was required to reach an ED₅₀ level. The N. cinerea bioassay presents a selective test for PSP toxicity that is rapid, economical, efficient, and simple to perform.
authors: HydroTox Services Australia Pty Ltd, Croydon, Victoria 3136, Australia. david_ruebhart@hydrotox.com.au
authors: David R
authors: DR
authors: Ruebhart
authors: Wendy L
authors: WL
authors: Radcliffe
authors: Geoffrey K
authors: GK
authors: Eaglesham
mesh_terms: D017033:Anabaena; D000818:Animals; D001681:Biological Assay / Q000379:methods*; D003058:Cockroaches / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004784:Environmental Monitoring / Q000379:methods; D008799:Mevinphos / Q000633:toxicity; D009420:Nervous System / Q000187:drug effects; D011042:Poisons / Q000302:isolation & purification / Q000633:toxicity*; D012530:Saxitoxin / Q000302:isolation & purification / Q000633:toxicity*; D057096:Shellfish Poisoning / Q000517:prevention & control
chemical_list: D011042:Poisons; D012530:Saxitoxin; D008799:Mevinphos