title: The effect of bee pollen on secretion activity, markers of proliferation and apoptosis of porcine ovarian granulosa cells in vitro.
abstract: The general objective of this in vitro study was to examine the effect of bee pollen on the release of insulin-like growth factor I (IGF-I) and steroid hormone progesterone, and expression of markers of proliferation (PCNA) and apoptosis (caspase-3) in porcine ovarian granulosa cells. Concentrations of IGF-I and progesterone were determined by RIA method and expression of PCNA and caspase-3 by immunocytochemistry. Bee pollen addition at the dose of 10 ng/mL significantly (P<0.05) inhibited IGF-I release by porcine ovarian granulosa cells. This growth factor was not influenced by 100 and 1000 ng/mL doses of bee pollen. Progesterone release by cells was not influenced by bee pollen addition at the doses of 10, 100 and 1000 ng/mL as used in our study. Similarly expression of PCNA and caspase-3 was not affected by bee pollen addition. The present study shows dose-dependent regulation of IGF-I by experimental bee pollen addition in vitro. Progesterone release, expression of PCNA and caspase-3 in porcine ovarian granulosa cells was not induced by pollen. Our results contribute to new insights regarding the possible effect of bee pollen on IGF-I release, which is important for regulation of porcine ovarian functions.
authors: Department of Animal Physiology, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic. adrianakolesarova@yahoo.com
authors: Adriana
authors: A
authors: Kolesarova
authors: Marcela
authors: M
authors: Capcarova
authors: Zuzana
authors: Z
authors: Bakova
authors: Branislav
authors: B
authors: Galik
authors: Miroslav
authors: M
authors: Juracek
authors: Milan
authors: M
authors: Simko
authors: Alexander V
authors: AV
authors: Sirotkin
mesh_terms: D000818:Animals; D017209:Apoptosis; D001516:Bees / Q000378:metabolism*; D053148:Caspase 3 / Q000378:metabolism; D049109:Cell Proliferation; D002478:Cells, Cultured; D005260:Female; D006107:Granulosa Cells / Q000166:cytology / Q000378:metabolism*; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D011058:Pollen; D011374:Progesterone / Q000378:metabolism; D013552:Swine
chemical_list: D011374:Progesterone; D007334:Insulin-Like Growth Factor I; D053148:Caspase 3