title: Impairment of human immunodeficiency virus type-1 integrase SUMOylation correlates with an early replication defect.
abstract: HIV-1 integrase (IN) orchestrates the integration of the reverse transcribed viral cDNA into the host cell genome and participates also in other steps of HIV-1 replication. Cellular and viral factors assist IN in performing its multiple functions, and post-translational modifications contribute to modulate its activities. Here, we show that HIV-1 IN is modified by SUMO proteins and that phylogenetically conserved SUMOylation consensus motifs represent major SUMO acceptor sites. Viruses harboring SUMOylation site IN mutants displayed a replication defect that was mapped during the early stages of infection, before integration but after reverse transcription. Because SUMOylation-defective IN mutants retained WT catalytic activity, we hypothesize that SUMOylation might regulate the affinity of IN for co-factors, contributing to efficient HIV-1 replication.
authors: CNRS UMR7212, INSERM U944, Institut Universitaire d'Hématologie-Université Paris7 Diderot, 75475 Paris, France. alessia.zamborlini@univ-paris-diderot.fr
authors: Alessia
authors: A
authors: Zamborlini
authors: Audrey
authors: A
authors: Coiffic
authors: Guillaume
authors: G
authors: Beauclair
authors: Olivier
authors: O
authors: Delelis
authors: Joris
authors: J
authors: Paris
authors: Yashuiro
authors: Y
authors: Koh
authors: Fabian
authors: F
authors: Magne
authors: Marie-Lou
authors: ML
authors: Giron
authors: Joelle
authors: J
authors: Tobaly-Tapiero
authors: Eric
authors: E
authors: Deprez
authors: Stephane
authors: S
authors: Emiliani
authors: Alan
authors: A
authors: Engelman
authors: Hugues
authors: H
authors: de Thé
authors: Ali
authors: A
authors: Saïb
mesh_terms: D057809:HEK293 Cells; D015658:HIV Infections / Q000235:genetics / Q000378:metabolism*; D019427:HIV Integrase / Q000235:genetics / Q000378:metabolism*; D015497:HIV-1 / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D009154:Mutation; D048348:Reverse Transcription / Q000502:physiology; D058207:Sumoylation / Q000502:physiology*; D016662:Virus Integration / Q000502:physiology*; D014779:Virus Replication / Q000502:physiology*
chemical_list: D019427:HIV Integrase