title: Autographa californica multiple nucleopolyhedrovirus odv-e66 is an essential gene required for oral infectivity.
abstract: Autographa californica multiple nucleopolyhedrovirus (AcMNPV) odv-e66 is a core gene and encodes an occlusion-derived virus (ODV)-specific envelope protein, ODV-E66. The N-terminal 23 amino acid of the envelope protein ODV-E66 are sufficient to direct native and fusion proteins to induced membrane microvesicles and the viral envelope during infection with AcMNPV. In this study, an odv-e66-knockout bacmid can not express N-terminal hydrophobic domains was constructed via homologous recombination in Escherichia coli. The odv-e66 deletion had no effect on budded virus (BV) production and viral DNA replication in infected Sf9 cells. Larval bioassays demonstrated that injection of odv-e66 deletion BV into the hemocoel could kill P. xylostella larvae as efficiently as repaired and control viruses; however, odv-e66 deletion mutant resulted in a 50% lethal dose that was 10(3) higher than that of the repaired and control viruses when inoculated per os. These results indicated that ODV-E66 envelope protein most likely played an important role in the oral infectivity of AcMNPV, but is not essential for virus replication.
authors: College of Animal Sciences, Zhejiang University, Huajiachi Campus, Hangzhou 310029, China.
authors: Xingwei
authors: X
authors: Xiang
authors: Lin
authors: L
authors: Chen
authors: Xiaolong
authors: X
authors: Hu
authors: Shaofang
authors: S
authors: Yu
authors: Rui
authors: R
authors: Yang
authors: Xiaofeng
authors: X
authors: Wu
mesh_terms: D000818:Animals; D001835:Body Weight; D002460:Cell Line; D004926:Escherichia coli / Q000235:genetics; D055786:Gene Knockout Techniques; D020043:Genes, Essential; D007814:Larva / Q000821:virology; D050296:Microbial Viability; D017924:Nucleopolyhedroviruses / Q000254:growth & development / Q000472:pathogenicity*; D018411:Spodoptera / Q000821:virology; D016019:Survival Analysis; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000378:metabolism*
chemical_list: D014759:Viral Envelope Proteins