title: The identification of novel protein, brain-derived integrating factor-1 (BDIF1), which interacts with astrocytic gap junctional protein.
abstract: Accumulating evidence demonstrates astrocytic crucial roles in the brain, but the molecular basis underlying astrocytic intracellular protein trafficking remains to be elucidated. The present study reports the identification of novel protein, brain-derived integrating factor-1 (BDIF1), which comprises TBC (Tre-2/Bub2/Cdc16), SH3, RUN domains. The amino acid sequence putatively coding TBC domain in BDIF1 implied its potential to interact with small GTP-binding proteins (G-proteins), and further analyses by co-immunprecipitation and immunocytochemical staining demonstrated that BDIF1 bound to astrocytic gap junctional protein, connexin-43 (Cx43). Our present data shows that BDIF1 potentially functions in molecular trafficking in astrocytes.
authors: Hamamatsu University School of Medicine, Department of Anatomy and Neuroscience, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan.
authors: Takeshi
authors: T
authors: Ito
authors: Takatoshi
authors: T
authors: Ueki
authors: Hiromu
authors: H
authors: Furukawa
authors: Kohji
authors: K
authors: Sato
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001253:Astrocytes / Q000378:metabolism* / Q000502:physiology; D001483:Base Sequence; D002450:Cell Communication / Q000502:physiology*; D018797:Cell Cycle Proteins; D018031:Connexin 43 / Q000378:metabolism* / Q000502:physiology; D017629:Gap Junctions / Q000378:metabolism* / Q000502:physiology; D057809:HEK293 Cells; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D021381:Protein Transport / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D018797:Cell Cycle Proteins; D018031:Connexin 43; D047908:Intracellular Signaling Peptides and Proteins; D009419:Nerve Tissue Proteins; C571820:brain-derived integrating factor-1, rat