title: Effects of shear stress on the number and function of endothelial progenitor cells adhered to specific matrices.
abstract: OBJECTIVE
  The aim of this study was to screen specific adherent matrix for endothelial progenitor cells (EPCs), which can be used for antibody capturing stents.
  
  
  METHODS
  In this study, the adhesion of EPCs on different matrices containing three different antibodies, VEGFR-2, CD34, CD133, was observed under shear stress in a flow chamber. Nitric oxide (NO) release, cell proliferation and the retention rate of EPCs, were measured separately.
  
  
  RESULTS
  The results demonstrated that shear stress within a certain range can promote proliferation and NO secretion of EPCs. Under the same shear stress, the EPCs showed stronger adhesion on matrix-containing CD133 antibody than on the other matrices.
  
  
  CONCLUSIONS
  CD133 antibody has the potential application for EPCs capture.
authors: Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, China.
authors: Li
authors: L
authors: Xiao
authors: Guixue
authors: G
authors: Wang
authors: Tao
authors: T
authors: Jiang
authors: Chaojun
authors: C
authors: Tang
authors: Xue
authors: X
authors: Wu
authors: Tin
authors: T
authors: Sun
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology*; D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion; D002454:Cell Differentiation; D049108:Cell Enlargement; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology* / Q000502:physiology*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology*; D051381:Rats; D033081:Shear Strength / Q000502:physiology
chemical_list: D000906:Antibodies; D001672:Biocompatible Materials