title: Chan-Yu-Bao-Yuan-Tang induces apoptosis in NSCLC and SCLC cell lines via a mitochondria-mediated pathway.
abstract: Previous clinical studies have shown efficacy and safety of the traditional Chinese medicinal herb extract Chan-Yu-Bao-Yuan-Tang (CYBYT) in lung cancer patients. The effects of CYBYT on proliferation and apoptosis in the non-small cell lung cancer cell line NCI-H460 and the small cell lung cancer cell line NCI-H446 were investigated in vitro. CYBYT significantly induced antiproliferative effects of NCI-H460 and NCI-H446 cells in a concentration- and time-dependent manner. The IC(50) values in NCI-H460 cells were 94.37 µg/mL (24 h) and 20.89 µg/mL (48 h), whereas in NCI-H446 cells IC(50) values were 214.72 µg/mL (24 h) and 114.58 µg/mL (48 h). Annexin V-FITC/PI staining showed CYBYT could significantly induce apoptosis in NCI-H460 and NCI-H446 cells, and the total apoptosis rates were positively correlated with the concentration and time of CYBYT treatment. Furthermore, treatment with the broad-spectrum caspase inhibitor (z-VAD-fmk) effectively protected NCI-H460 and NCI-H446 cells against CYBYT-triggered apoptosis. The apoptotic processes involved were a marked decrease in antiapoptotic protein Bcl-2 and an increase in proapoptotic protein Bax. The release of mitochondrial cytochrome c into the cytosol was also observed, which, in turn, resulted in the activation of caspase-9 and caspase-3. CYBYT exerts antiproliferative and growth inhibition effects on NCI-H460 and NCI-H446 cells through the mitochondrial caspase-dependent cell death pathway.
authors: Joint Laboratory of Immunogenomics, Zhoushan Hospital-Beijing Institute of Genomics, Chinese Academy of Sciences, Zhoushan, China.
authors: Yongkui
authors: Y
authors: Zhang
authors: Fang
authors: F
authors: Zeng
authors: XiaoGuang
authors: X
authors: Liu
authors: YunCheng
authors: Y
authors: Li
authors: JiHang
authors: J
authors: Zhou
authors: YanYan
authors: Y
authors: Huang
authors: Yekai
authors: Y
authors: Wang
authors: ShiQuan
authors: S
authors: Zhou
authors: WangYu
authors: W
authors: Zhu
authors: ErYun
authors: E
authors: Shu
authors: Guoer
authors: G
authors: Zhou
authors: Gang
authors: G
authors: Chen
mesh_terms: D017304:Annexin A5 / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects*; D002289:Carcinoma, Non-Small-Cell Lung / Q000188:drug therapy / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy / Q000473:pathology*; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D011419:Propidium / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*; D055752:Small Cell Lung Carcinoma / Q000188:drug therapy / Q000473:pathology*
chemical_list: D017304:Annexin A5; D004365:Drugs, Chinese Herbal; C569176:chan-yu-bao-yuan-tang; D011419:Propidium; D016650:Fluorescein-5-isothiocyanate