title: RNAi-mediated COPS3 gene silencing inhibits metastasis of osteogenic sarcoma cells.
abstract: Metastatic disease is the primary cause of mortality among patients with osteogenic sarcoma (OGS). In this study, we aimed to identify the relationship of COPS3 gene expression to metastasis. Immunohistochemical staining for COPS3 was performed on 65 OGS samples (37 without and 28 with metastatic disease); 18.9% (7/37) of specimens from patients with no metastasis and 57.1% (16/28) of specimens from patients with metastasis showed intense staining of COPS3. Comparison of COPS3 expression between a poorly metastatic osteosarcoma cell line (SAOS-2) and highly metastatic osteosarcoma cell line (HOS) showed stronger expression of COPS3 in HOS cells. Inhibiting COPS3 function by siRNA resulted in reduced proliferation and migration of HOS cells. Inhibition of COPS3 gene downregulated expression of the MAPK signaling pathway, which has an important role in metastasis of OGS. Our results suggested that overexpression of the COPS3 gene might have important roles in metastasis of osteosarcoma cells.
authors: Musculoskeletal Tumor Center, Peking University People's Hospital, Beijing, China.
authors: T
authors: T
authors: Yan
authors: G
authors: G
authors: Tang
authors: T
authors: T
authors: Ren
authors: D
authors: D
authors: Shen
authors: K
authors: K
authors: Sun
authors: W
authors: W
authors: Liang
authors: W
authors: W
authors: Guo
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001859:Bone Neoplasms / Q000235:genetics / Q000473:pathology*; D000075686:COP9 Signalosome Complex; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D012516:Osteosarcoma / Q000235:genetics / Q000556:secondary*; D011518:Proto-Oncogene Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D015398:Signal Transduction
chemical_list: C477661:COPS3 protein, human; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D000075686:COP9 Signalosome Complex