title: A proton NMR study of the effect of Mucuna pruriens on seminal plasma metabolites of infertile males.
abstract: The objective of this study was to employ proton nuclear magnetic resonance ((1)H NMR) spectroscopy to evaluate the impact of Mucuna pruriens seeds on the metabolic profile of seminal plasma of infertile patients. A total of 180 infertile patients were administered M. pruriens seed powder for a period of three months. Age-matched healthy men comprised the control (n=50) group in the study. Lactate, alanine, choline, citrate, glycerophosphocholine (GPC), glutamine, tyrosine, histidine, phenylalanine, and uridine were measured in seminal plasma by (1)H NMR spectroscopy. To evaluate the degree of infertility and extent of hormonal imbalance induced by this milieu, separate sperm concentration, motility, lipid peroxide in seminal plasma and LH, FSH, T, and PRL hormone concentration in serum were measured using standard laboratory methods and RIA, respectively, in the same subjects. M. pruriens therapy rectifies the perturbed alanine, citrate, GPC, histidine and phenylalanine content in seminal plasma and improves the semen quality of post-treated infertile men with compared to pre-treated. Concomitantly, clinical variables in seminal plasma and blood serum were also improved over post therapy in infertile men. On the basis of these observations, it may be proposed that M. pruriens seed powder not only reactivates the enzymatic activity of metabolic pathways and energy metabolism but also rejuvenates the harmonic balance of male reproductive hormones in infertile men. These findings open more opportunities for infertility treatment and management by improving semen quality.
authors: Department of Biochemistry, Chhatrapati Shahuji Maharaj Medical University, Lucknow, India.
authors: Ashish
authors: A
authors: Gupta
authors: Abbas Ali
authors: AA
authors: Mahdi
authors: Mohammad Kaleem
authors: MK
authors: Ahmad
authors: Kamla Kant
authors: KK
authors: Shukla
authors: Navneeta
authors: N
authors: Bansal
authors: Shyam Pyari
authors: SP
authors: Jaiswer
authors: Satya Narain
authors: SN
authors: Shankhwar
mesh_terms: D000328:Adult; D053627:Asthenozoospermia / Q000188:drug therapy; D016022:Case-Control Studies; D006801:Humans; D007248:Infertility, Male / Q000628:therapy*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D008875:Middle Aged; D015233:Models, Statistical; D031299:Mucuna / Q000378:metabolism*; D009845:Oligospermia / Q000188:drug therapy; D010936:Plant Extracts / Q000032:analysis* / Q000494:pharmacology; D011422:Propionates / Q000032:analysis; D011522:Protons; D012661:Semen / Q000187:drug effects* / Q000378:metabolism; D013081:Sperm Motility / Q000187:drug effects; D014297:Trimethylsilyl Compounds / Q000032:analysis
chemical_list: C523397:3-(trimethylsilyl)propionic acid; D010936:Plant Extracts; D011422:Propionates; D011522:Protons; D014297:Trimethylsilyl Compounds