title: [Equol induced apoptosis of human breast cancer MDA-MB-231 cell by inhibiting the expression of nuclear factor-kappaB].
abstract: OBJECTIVE
  To study the inhibition effect of equol on MDA-MB-231 cells, a human breast cancer cell line with estrogen-independent receptor.
  
  
  METHODS
  Cultured MDA-MB-231 cells were treated with different contents of equol. The cell viability was assessed with MTT method. The apoptosis was detected by flow cytometry and immunofluorescence. Western Blot was used to assess the expression of NF-kappaB.
  
  
  RESULTS
  The growth of MDA-MB-231 cells was inhibited by equol in a does-and time-dependent manner. The apoptosis of MDA-MB-231 cells induced by equol was the result of inhibiting the expression of NF-kappaB.
  
  
  CONCLUSIONS
  The apoptosis of MDA-MB-231 cells might be the reduced expression of NF-kappaB induced by equol.
authors: Department of Nutrition and Food Hygiene, Fourth Military Medical University, Xi'an 710032, China. jianingshi0301@163.com
authors: Jianing
authors: J
authors: Shi
authors: Ailing
authors: A
authors: Ji
authors: Zipeng
authors: Z
authors: Cao
authors: Rui
authors: R
authors: Cao
authors: Dan
authors: D
authors: Li
authors: Ruihua
authors: R
authors: Yang
authors: Feng
authors: F
authors: Wang
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D060754:Equol / Q000494:pharmacology*; D005260:Female; D006801:Humans; D016328:NF-kappa B / Q000378:metabolism*; D048789:Phytoestrogens / Q000494:pharmacology
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D016328:NF-kappa B; D048789:Phytoestrogens; D060754:Equol