title: pH-controlled recovery of placenta-derived mesenchymal stem cell sheets.
abstract: Widely used in different biomedical applications, polyelectrolyte multilayers provide inter alia an attractive way for manufacturing of bio-functionalized, stimuli responsive surface coatings to control cellular behavior. In this study a novel polyelectrolyte-based platform for the engineering and controllable detachment of human mesenchymal stem cell (MSC) sheets is presented. Thin films obtained by layer-by-layer deposition of cationic poly(allylamine hydrochloride) (PAH) and anionic poly(styrene sulfonate) (PSS) polyelectrolytes on conductive indium tin oxide (ITO) electrodes allowed for the fast formation of viable sheets from human placenta-derived mesenchymal stem cells (PD-MSCs). Resulting stem cell sheets retained their phenotypical profile and mesodermal differentiation potency. Both electrochemically-induced local pH lowering and global decrease of the environmental pH allowed for a rapid detachment of intact stem cell sheets. The recovered stem cell sheets remained viable and maintained their capacity to differentiate toward the adipogenic and osteogenic lineages. This novel polyelectrolyte multilayer based platform represents a promising, novel approach for the engineering of human stem cell sheets desired for future clinical applications.
authors: Laboratory of Biosensors and Bioelectronics, Institute for Biomedical Engineering, ETH Zurich, Switzerland.
authors: Orane
authors: O
authors: Guillaume-Gentil
authors: Oleg V
authors: OV
authors: Semenov
authors: Andreas H
authors: AH
authors: Zisch
authors: Roland
authors: R
authors: Zimmermann
authors: Janos
authors: J
authors: Vörös
authors: Martin
authors: M
authors: Ehrbar
mesh_terms: D001672:Biocompatible Materials; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D002478:Cells, Cultured; D055664:Electrochemical Techniques; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D018625:Microscopy, Atomic Force; D010920:Placenta / Q000166:cytology*; D011247:Pregnancy; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D001672:Biocompatible Materials