title: Stromal vascular fraction transplantation as an alternative therapy for ischemic heart failure: anti-inflammatory role.
abstract: BACKGROUND
  The aims of this study were: (1) to show the feasibility of using adipose-derived stromal vascular fraction (SVF) as an alternative to bone marrow mono nuclear cell (BM-MNC) for cell transplantation into chronic ischemic myocardium; and (2) to explore underlying mechanisms with focus on anti-inflammation role of engrafted SVF and BM-MNC post chronic myocardial infarction (MI) against left ventricular (LV) remodelling and cardiac dysfunction.
  
  
  METHODS
  Four weeks after left anterior descending coronary artery ligation, 32 Male Lewis rats with moderate MI were divided into 3 groups. SVF group (n = 12) had SVF cell transplantation (6 × 10(6) cells). BM-MNC group (n = 12) received BM-MNCs (6 × 10(6)) and the control (n = 10) had culture medium. At 4 weeks, after the final echocardiography, histological sections were stained with Styrus red and immunohistochemical staining was performed for α-smooth muscle actin, von Willebrand factor, CD3, CD8 and CD20.
  
  
  RESULTS
  At 4 weeks, in SVF and BM-MNC groups, LV diastolic dimension and LV systolic dimension were smaller and fractional shortening was increased in echocardiography, compared to control group. Histology revealed highest vascular density, CD3+ and CD20+ cells in SVF transplanted group. SVF transplantation decreased myocardial mRNA expression of inflammatory cytokines TNF-α, IL-6, MMP-1, TIMP-1 and inhibited collagen deposition.
  
  
  CONCLUSIONS
  Transplantation of adipose derived SVF cells might be a useful therapeutic option for angiogenesis in chronic ischemic heart disease. Anti-inflammation role for SVF and BM transplantation might partly benefit for the cardioprotective effect for chronic ischemic myocardium.
authors: Wallenberg Laboratory for Cardiovascular Research, Sahlgrenska University Hospital, University of Gothenburg, Gothenburg, Sweden. goditha@gmail.com
authors: Goditha U
authors: GU
authors: Premaratne
authors: Li-Ping
authors: LP
authors: Ma
authors: Masatoshi
authors: M
authors: Fujita
authors: Xue
authors: X
authors: Lin
authors: Entela
authors: E
authors: Bollano
authors: Michael
authors: M
authors: Fu
mesh_terms: D000818:Animals; D001808:Blood Vessels / Q000166:cytology*; D017690:Cell Transplantation / Q000379:methods*; D004195:Disease Models, Animal; D004452:Echocardiography; D006333:Heart Failure / Q000150:complications / Q000000981:diagnostic imaging / Q000601:surgery*; D007150:Immunohistochemistry; D008297:Male; D017202:Myocardial Ischemia / Q000150:complications / Q000000981:diagnostic imaging / Q000601:surgery*; D051381:Rats; D011917:Rats, Inbred Lew; D017154:Stromal Cells / Q000637:transplantation*