title: [Relaxant activity of aqueous and ethanol extracts of parsley (Petroselinum crispum (Mill) Nym. ex A. W Hill, Apiaceae) on isolated ileum of rat].
abstract: BACKGROUND
  Parsley (Petroselinum crispum) is used in the traditional herbal medicine to treat intestinal disorders. The aim of this study was to examine the effect of aqueous and ethanol extracts of parsley on spontaneous and acetylcholine induced contractions on isolated rat ileum.
  
  
  METHODS
  Wistar albino rats (250-300 g) were used in this study. The ileum portions were isolated out and cleaned off mesenteries. Preparations 2 cm long were mounted in 20 ml tissue baths containing Tyrode's solution maintained at 37 degrees C and aerated with a mixture of 5% carbon dioxide in oxygen. In the first part of experiments, contractile responses to the aqueous (ethanol) extracts of parsley were recorded. In the second part, increasing concentrations of acetylcholine were added to the organ bath for a full concentration response curve and then concentration response curves were obtained after adding the aqueous (ethanol) extracts of parsley.
  
  
  CONCLUSIONS
  Our results showed that aqueous (62.22 +/- 7.15%) and ethanol (79.16 +/- 9.34%) extracts of parsley in dose dependent manner decreased the tonus of spontaneous contractions of isolated rat ileum. The aqueous (32.16 +/- 2.75%) and ethanol (53.96 +/- 4.86%) extracts of parsley reduced the acetylcholine induced contraction, the reduction was greater with ethanol extract than with the aqueous one.
  
  
  CONCLUSIONS
  It can be concluded that the aqueous and ethanol extracts of parsley exert antispasmodic activity on rat ileum. The relaxant effect of ethanol extract was better comparing to aqueous extract of parsley.
authors: Institut za fiziologiju, Medicinski fakultet, Nis.
authors: Suzana
authors: S
authors: Branković
authors: Dusanka
authors: D
authors: Kitić
authors: Mirjana
authors: M
authors: Radenković
authors: Vesna
authors: V
authors: Ivetić
authors: Slavimir
authors: S
authors: Veljković
authors: Milkica
authors: M
authors: Nesić
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D007082:Ileum / Q000187:drug effects*; D066298:In Vitro Techniques; D009119:Muscle Contraction / Q000187:drug effects*; D010276:Parasympatholytics / Q000494:pharmacology*; D028528:Petroselinum; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D010276:Parasympatholytics; D010936:Plant Extracts