title: Jasmonate signaling involves the abscisic acid receptor PYL4 to regulate metabolic reprogramming in Arabidopsis and tobacco.
abstract: The phytohormones jasmonates (JAs) constitute an important class of elicitors for many plant secondary metabolic pathways. However, JAs do not act independently but operate in complex networks with crosstalk to several other phytohormonal signaling pathways. Here, crosstalk was detected between the JA and abscisic acid (ABA) signaling pathways in the regulation of tobacco (Nicotiana tabacum) alkaloid biosynthesis. A tobacco gene from the PYR/PYL/RCAR family, NtPYL4, the expression of which is regulated by JAs, was found to encode a functional ABA receptor. NtPYL4 inhibited the type-2C protein phosphatases known to be key negative regulators of ABA signaling in an ABA-dependent manner. Overexpression of NtPYL4 in tobacco hairy roots caused a reprogramming of the cellular metabolism that resulted in a decreased alkaloid accumulation and conferred ABA sensitivity to the production of alkaloids. In contrast, the alkaloid biosynthetic pathway was not responsive to ABA in control tobacco roots. Functional analysis of the Arabidopsis (Arabidopsis thaliana) homologs of NtPYL4, PYL4 and PYL5, indicated that also in Arabidopsis altered PYL expression affected the JA response, both in terms of biomass and anthocyanin production. These findings define a connection between a component of the core ABA signaling pathway and the JA responses and contribute to the understanding of the role of JAs in balancing tradeoffs between growth and defense.
authors: VTT Technical Research Center of Finland, FIN-02044 VTT Espoo, Finland.
authors: Petri
authors: P
authors: Lackman
authors: Miguel
authors: M
authors: González-Guzmán
authors: Sofie
authors: S
authors: Tilleman
authors: Inês
authors: I
authors: Carqueijeiro
authors: Amparo Cuéllar
authors: AC
authors: Pérez
authors: Tessa
authors: T
authors: Moses
authors: Mitsunori
authors: M
authors: Seo
authors: Yuri
authors: Y
authors: Kanno
authors: Suvi T
authors: ST
authors: Häkkinen
authors: Marc C E
authors: MC
authors: Van Montagu
authors: Johan M
authors: JM
authors: Thevelein
authors: Hannu
authors: H
authors: Maaheimo
authors: Kirsi-Marja
authors: KM
authors: Oksman-Caldentey
authors: Pedro L
authors: PL
authors: Rodriguez
authors: Heiko
authors: H
authors: Rischer
authors: Alain
authors: A
authors: Goossens
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism*; D000470:Alkaloids / Q000096:biosynthesis; D054458:Amplified Fragment Length Polymorphism Analysis; D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D003517:Cyclopentanes / Q000378:metabolism*; D020869:Gene Expression Profiling; D054883:Oxylipins / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000502:physiology*; D014026:Nicotiana / Q000378:metabolism*
chemical_list: D000470:Alkaloids; D029681:Arabidopsis Proteins; D003517:Cyclopentanes; D054883:Oxylipins; C587488:PYL4 protein, Arabidopsis; D010937:Plant Growth Regulators; D011956:Receptors, Cell Surface; C011006:jasmonic acid; D000040:Abscisic Acid