title: Pharmacological doses of melatonin reduce the glycosaminoglycan level within the infarcted heart scar.
abstract: The aim of the study was to define the effect of pharmacological doses of melatonin, an agent known to be a scavenger of reactive oxygen species, on the extracellular matrix composition (glycosaminoglycans and collagen) in the infarcted heart scar. Rats were administered with melatonin at doses of 300 μg/100 g b.w. or 3 mg/100 g b.w. once daily (between 5:00 and 6:00 in the afternoon) or with 1.5 mg/100 g b.w. twice daily (between 8:00 and 9:00 in the morning and additionally between 5:00 and 6:00 in the afternoon). The levels of collagen, glycosaminoglycans (GAG) and some oxidative stress markers (lipid oxidation, the content of sulphydryl groups in proteins and glutathione) were evaluated. In the second part of the experiment, cells were isolated from the scar, identified as myofibroblasts, cultured and treated with melatonin at concentrations ranging from 10⁻⁷ M to 10⁻¹⁰ M. The pineal indoleamine was seen to reduce the GAG content of the scar, while the collagen content of the scar remained unchanged. A 10⁻⁷ M concentration of melatonin caused an increase in the GAG level in the myofibroblast cultures, while lower concentrations (10⁻⁸ M-10⁻¹⁰ M) of pineal indoleamine were not effective. Melatonin decreased lipid oxidation and increased the sulphydryl groups of total proteins and glutathione, which suggests its antioxidative activity in the applied doses. The present study shows that pharmacological doses of melatonin reduce the GAG level in an infarcted heart scar. Since the mechanism of GAG content reduction cannot be explained by direct action of the pineal indoleamine on myofibroblasts in the myocardial infarction scar, we hypothesise that changes in GAG content could be indirectly induced by melatonin, that is caused by changes in regulatory systems or reduction of the inflammatory reaction in the area of the infarction. In addition, this paper shows that long-term treatment with melatonin of rats affected by myocardial infarction may reduce oxidative stress in the infarction area.
authors: Department of Connective Tissue Metabolism, Medical University of Lodz, 90-136 Lodz, Poland. drob@op.pl
authors: J
authors: J
authors: Drobnik
authors: D
authors: D
authors: Slotwinska
authors: S
authors: S
authors: Olczak
authors: D
authors: D
authors: Tosik
authors: A
authors: A
authors: Pieniazek
authors: K
authors: K
authors: Matczak
authors: A
authors: A
authors: Koceva-Chyla
authors: A
authors: A
authors: Szczepanowska
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002921:Cicatrix / Q000378:metabolism* / Q000473:pathology; D003094:Collagen / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D006025:Glycosaminoglycans / Q000378:metabolism*; D006321:Heart / Q000187:drug effects; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008550:Melatonin / Q000008:administration & dosage*; D009203:Myocardial Infarction / Q000378:metabolism* / Q000473:pathology; D058628:Myofibroblasts / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D018384:Oxidative Stress / Q000187:drug effects; D010870:Pineal Gland / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D006025:Glycosaminoglycans; D003094:Collagen; D005978:Glutathione; D008550:Melatonin