title: Antiserum to the recombinant truncated VP22 protein of herpes simplex virus type 1 that also recognizes full-length VP22.
abstract: The herpes simplex virus type 1 (HSV-1) tegument protein VP22 encoded by the UL49 gene is essential for HSV-1 infection. However, its precise functions in the virus life cycle are unknown. A relatively important tool for disclosing these functions is an antiserum specifically detecting VP22 in the infected cell. To this end, a recombinant truncated VP22 protein consisting of C-terminal 45 aa fused to EYFP (enhanced yellow fluorescent protein) and His-tag was expressed in Escherichia coli, purified by the Ni2+-NTA affinity chromatography, and used for the preparation of antiserum in rabbits. Western blot and immunofluorescence assay showed that this antiserum specifically detected purified truncated VP22 as well as full-length VP22 in the HSV-1 infected cells. These results indicate that the prepared antiserum could serve as a valuable tool for further studies of VP22 functions.
authors: Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, People's Republic of China.
authors: M L
authors: ML
authors: Li
authors: S
authors: S
authors: Wang
authors: J J
authors: JJ
authors: Xing
authors: Ch
authors: Ch
authors: Zheng
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000276:immunology*; D007106:Immune Sera / Q000276:immunology*; D008164:Luminescent Proteins / Q000737:chemistry; D010446:Peptide Fragments / Q000235:genetics / Q000276:immunology; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D015678:Viral Structural Proteins / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification
chemical_list: D001426:Bacterial Proteins; D007106:Immune Sera; D008164:Luminescent Proteins; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; D015678:Viral Structural Proteins; C073413:herpes simplex virus type 1 protein VP22; C054562:yellow fluorescent protein, Bacteria