title: Visualization of mRNA localization in Xenopus oocytes.
abstract: Visualization of in vivo mRNA localization provides a tool for understanding steps in the mechanism of transport. Here we detail a method of fluorescently labeling mRNA transcripts and microinjecting them into Xenopus laevis oocytes followed with imaging by confocal microscopy. This technique overcomes a significant hurdle of imaging RNA in the frog oocyte while providing a rapid method of visualizing mRNA localization in high resolution.
authors: Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, RI, USA.
authors: James A
authors: JA
authors: Gagnon
authors: Kimberly L
authors: KL
authors: Mowry
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D005260:Female; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes / Q000378:metabolism; D008845:Microinjections; D018613:Microscopy, Confocal; D057054:Molecular Imaging / Q000379:methods*; D009865:Oocytes / Q000166:cytology / Q000378:metabolism*; D034443:RNA Transport; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D014982:Xenopus laevis / Q000235:genetics*
chemical_list: D005456:Fluorescent Dyes; D012333:RNA, Messenger