title: Selectable genetic markers for nematode transgenesis.
abstract: The nematode Caenorhabditis elegans has been used to study genetics and development since the mid-1970s. Over the years, the arsenal of techniques employed in this field has grown steadily in parallel with the number of researchers using this model. Since the introduction of C. elegans transgenesis, nearly 20 years ago, this system has been extensively used in areas such as rescue experiments, gene expression studies, and protein localization. The completion of the C. elegans genome sequence paved the way for genome-wide studies requiring higher throughput and improved scalability than provided by traditional genetic markers. The development of antibiotic selection systems for nematode transgenesis addresses these requirements and opens the possibility to apply transgenesis to investigate biological functions in other nematode species for which no genetic markers had been developed to date.
authors: Genome Regulation and Evolution, Inserm U869, Université de Bordeaux, Institut Européen de Chimie et Biologie (IECB), Pessac 33607, France.
authors: Rosina
authors: R
authors: Giordano-Santini
authors: Denis
authors: D
authors: Dupuy
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics*; D004352:Drug Resistance, Microbial / Q000235:genetics; D018014:Gene Transfer Techniques; D005819:Genetic Markers; D008957:Models, Genetic
chemical_list: D005819:Genetic Markers