title: [The role of light in the developement of RPE degeneration in AMD and potential cytoprotection of minocycline].
abstract: BACKGROUND
  Light-induced oxidative stress is an suggested reason for retinal pigment epithelium (RPE) degeneration in age-related macular degeneration (AMD). This study investigates the influence of light on intracellular reactive oxygen species (ROS) and apoptosis in the human RPE and potential cytoprotective effects of the tetracycline antibiotic minocycline.
  
  
  METHODS
  Primary human RPE cells were either pre- or post-incubated with minocycline and then exposed to white light or oxidative stress (600 µM, H(2)O(2)). Then viability, induction of intracellular reactive oxygen species (ROS), apoptosis and cell death was determined. Expression of apoptotic BAX and anti-apoptotic Bcl-2 protein and their mRNA were determined by RT-PCR and Western blot analysis.
  
  
  RESULTS
  Both light exposure and oxidative stress decreased RPE cell viability and Bcl-2 expression and increased intracellular ROS, apoptotic cell death, and BAX expression. Minocycline reduced these effects under certain conditions.
  
  
  CONCLUSIONS
  This study demonstrates that minocycline effectively protects human RPE cells against oxidative damage. However, in the light of minocycline's photosensitising properties its potential role in AMD treatment needs further evaluation.
authors: Augenklinik, Ludwig-Maximilians-Universität München, München. marcus.kernt@med.unimuenchen.de
authors: M
authors: M
authors: Kernt
authors: S
authors: S
authors: Thiele
authors: C
authors: C
authors: Hirneiss
authors: A S
authors: AS
authors: Neubauer
authors: C-A
authors: CA
authors: Lackerbauer
authors: M W
authors: MW
authors: Ulbig
authors: A
authors: A
authors: Kampik
mesh_terms: D000900:Anti-Bacterial Agents / Q000009:adverse effects / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D015153:Blotting, Western; D016923:Cell Death / Q000187:drug effects / Q000502:physiology; D049109:Cell Proliferation; D002478:Cells, Cultured / Q000187:drug effects; D019610:Cytoprotection / Q000187:drug effects*; D006801:Humans; D008027:Light / Q000009:adverse effects; D008268:Macular Degeneration / Q000175:diagnosis / Q000188:drug therapy* / Q000235:genetics / Q000503:physiopathology*; D008911:Minocycline / Q000009:adverse effects / Q000627:therapeutic use*; D010857:Pigment Epithelium of Eye / Q000187:drug effects / Q000503:physiopathology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051028:bcl-2-Associated X Protein / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; C494826:BAX protein, human; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D051028:bcl-2-Associated X Protein; D008911:Minocycline