title: Photoacoustic tomography of ex vivo mouse hearts with myocardial infarction.
abstract: In the present study, we evaluated the applicability of ex vivo photoacoustic imaging (PAI) on small animal organs. We used photoacoustic tomography (PAT) to visualize infarcted areas within murine hearts and compared these data to other imaging techniques [magnetic resonance imaging (MRI), micro-computed tomography] and histological slices. In order to induce ischemia, an in vivo ligation of the left anterior descending artery was performed on nine wild-type mice. After varying survival periods, the hearts were excised and fixed in formaldehyde. Samples were illuminated with nanosecond laser pulses delivered by a Nd:YAG pumped optical parametric oscillator. Ultrasound detection was achieved using a Mach-Zehnder interferometer (MZI) working as an integrating line detector. The voxel data were computed using a Fourier-domain based reconstruction algorithm, followed by inverse Radon transforms. The results clearly showed the capability of PAI to visualize myocardial infarction and to produce three-dimensional images with a spatial resolution of approximately 120 μm. Regions of affected muscle tissue in PAI corresponded well with the results of MRI and histology. Photoacoustic tomography utilizing a MZI for ultrasound detection allows for imaging of small tissue samples. Due to its high spatial resolution, good soft tissue contrast and comparatively low cost, PAT offers great potentials for imaging.
authors: Innsbruck Medical University, Department of Radiology, Innsbruck, Austria. markus.holotta@i-med.ac.at
authors: Markus
authors: M
authors: Holotta
authors: Harald
authors: H
authors: Grossauer
authors: Christian
authors: C
authors: Kremser
authors: Pavle
authors: P
authors: Torbica
authors: Jakob
authors: J
authors: Völkl
authors: Gerald
authors: G
authors: Degenhart
authors: Regina
authors: R
authors: Esterhammer
authors: Robert
authors: R
authors: Nuster
authors: Günther
authors: G
authors: Paltauf
authors: Werner
authors: W
authors: Jaschke
mesh_terms: D000162:Acoustics; D000818:Animals; D004195:Disease Models, Animal; D006652:Histological Techniques; D021621:Imaging, Three-Dimensional; D007368:Interferometry; D008279:Magnetic Resonance Imaging; D051379:Mice; D008810:Mice, Inbred C57BL; D009203:Myocardial Infarction / Q000175:diagnosis* / Q000000981:diagnostic imaging; D055620:Optical Phenomena; D041622:Tomography, Optical / Q000379:methods*; D014463:Ultrasonography; D055114:X-Ray Microtomography