title: Portable device based on chemiluminescence lensless imaging for personalized diagnostics through multiplex bioanalysis.
abstract: A simple and versatile analytical device designed to perform, even simultaneously, different types of bioassays has been developed and optimized. A transparent microfluidics-based reaction chip, where analytes were quantitatively detected by means of biospecific reactions and chemiluminescence detection, was placed in contact with a thermoelectrically cooled CCD sensor through a fiber optic taper. Such a lensless contact imaging configuration combined adequate spatial resolution and high light collection efficiency within a small size portable device. The miniaturization of the reaction chamber ensured short analysis times (in the minutes range), while the use of chemiluminescence detection provided wide signal dynamic range and high detectability, down to attomole levels of protein and femtomole levels of nucleic acid analytes. A model hybrid panel test was realized by combining an enzyme assay for alkaline phosphatase activity, a nucleic acid hybridization assay for Parvovirus B19 DNA, and an immunoassay for horseradish peroxidase as a model antigen. The successful simultaneous quantification of the three targets demonstrated that a range of analytes, from enzymes to antigens, antibodies, and nucleic acids, can be measured in a single run, thus enabling the realization of a complete, personalized diagnostic panel test for early diagnosis of a given disease and patient follow-up.
authors: Department of Pharmaceutical Sciences, University of Bologna, Via Belmeloro 6, 40126 Bologna, Italy. aldo.roda@unibo.it
authors: Aldo
authors: A
authors: Roda
authors: Mara
authors: M
authors: Mirasoli
authors: Luisa Stella
authors: LS
authors: Dolci
authors: Angela
authors: A
authors: Buragina
authors: Francesca
authors: F
authors: Bonvicini
authors: Patrizia
authors: P
authors: Simoni
authors: Massimo
authors: M
authors: Guardigli
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis / Q000378:metabolism; D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002417:Cattle; D004279:DNA, Viral / Q000032:analysis; D004867:Equipment Design; D006735:Horseradish Peroxidase / Q000032:analysis / Q000378:metabolism; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D009696:Nucleic Acids / Q000032:analysis; D057285:Precision Medicine / Q000295:instrumentation* / Q000379:methods; D012710:Serum Albumin, Bovine / Q000032:analysis
chemical_list: D004279:DNA, Viral; D009696:Nucleic Acids; D012710:Serum Albumin, Bovine; D006735:Horseradish Peroxidase; D000469:Alkaline Phosphatase