title: Expression of a recombinant chimeric protein of hepatitis A virus VP1-Fc using a replicating vector based on Beet curly top virus in tobacco leaves and its immunogenicity in mice.
abstract: We describe the expression and immunogenicity of a recombinant chimeric protein (HAV VP1-Fc) consisting of human hepatitis A virus VP1 and an Fc antibody fragment using a replicating vector based on Beet curly top virus (BCTV) in Agrobacterium-infiltrated Nicotiana benthamiana leaves. Recombinant HAV VP1-Fc was expressed with a molecular mass of approximately 68 kDa. Recombinant HAV VP1-Fc, purified using Protein A Sepharose affinity chromatography, elicited production of specific IgG antibodies in the serum after intraperitoneal immunization. Following vaccination with recombinant HAV VP1-Fc protein, expressions of IFN-γ and IL-4 were increased in splenocytes at the time of sacrifice. Recombinant VP1-Fc from infiltrated tobacco plants can be used as an effective experimental immunogen for research into vaccine development.
authors: Department of Genetic Engineering, Graduate School of Biotechnology, Kyung Hee University, Yongin, 446-701, Korea.
authors: Ho Yong
authors: HY
authors: Chung
authors: Hyun Ho
authors: HH
authors: Lee
authors: Kyung Il
authors: KI
authors: Kim
authors: Ha Young
authors: HY
authors: Chung
authors: Jeon
authors: J
authors: Hwang-Bo
authors: Jong Hwa
authors: JH
authors: Park
authors: Garry
authors: G
authors: Sunter
authors: Jong Bum
authors: JB
authors: Kim
authors: Dong Hwa
authors: DH
authors: Shon
authors: Wonyong
authors: W
authors: Kim
authors: In Sik
authors: IS
authors: Chung
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002478:Cells, Cultured; D003429:Cross Reactions; D005260:Female; D005822:Genetic Vectors; D030041:Hepatitis A virus; D007114:Immunization; D007141:Immunoglobulin Fc Fragments / Q000096:biosynthesis* / Q000276:immunology; D007371:Interferon-gamma / Q000276:immunology; D015847:Interleukin-4 / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010942:Plant Viruses / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000821:virology; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000276:immunology; D014026:Nicotiana / Q000235:genetics* / Q000821:virology; D015678:Viral Structural Proteins / Q000096:biosynthesis* / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D007141:Immunoglobulin Fc Fragments; D011993:Recombinant Fusion Proteins; C096539:VP1 protein, hepatitis A virus; D015678:Viral Structural Proteins; D015847:Interleukin-4; D007371:Interferon-gamma