title: Maternal/newborn genotype contribution of the renin-angiotensin system (Met235Thr, Thr174Met, I/D-ACE, A2350G-ACE, A1166C-AT2R1, C3123A- AT2R2, 83A/G-REN) to the risk of pre-eclampsia: a Romanian study.
abstract: BACKGROUND
  We evaluated the association of the mutated genotypes Met235Thr-AGT, Thr174Met-AGT, I/D-ACE, A2350G-ACE, A1166C-AT2R1, C3123A-AT2R2, (83)A/G-REN with the risk and outcome of pre-eclampsia; we also investigated whether genes in newborns increase maternal risk of pre-eclampsia.
  
  
  METHODS
  Thirty-six pairs of pre-eclamptic women and their newborns were genotyped, along with 71 pairs of controls (mothers/newborns) using PCR-RFLP analysis.
  
  
  RESULTS
  The Thr235/Thr235 (OR 3.44, p = 0.01), DD (OR 2.66, p = 0.039), CC1166 (OR 5.56, p = 0.04), AA3123 (OR 3.77, p = 0.03) and GG(83) (OR 8.32, p = 0.006) genotypes are significantly associated with pre-eclampsia. Women with pre-eclampsia positive for Met235Thr (34.64 ± 3.92 weeks vs. 38 ± 2 weeks), Thr174Met (32.58 ± 3.92 weeks vs. 36.38 ± 3.25 weeks), I/D (34.47 ± 3.67 weeks vs. 38.33 ± 3.5 weeks) delivered at a significant lower gestational age compared with pre-eclamptic women with a normal genotype. Newborns from women with pre-eclampsia positive for Thr174Met (2190 ± 820.21 g vs. 2702.08 ± 967.23 g), I/D (2399.33 ± 938.38 g vs. 3191.66 ± 684.40 g) had a significant lower birth weight compared with newborns from women with normal pregnancies. When both the mother and the newborn were positive for Met235Thr, I/D, A2350G, A1166C or (83)A/G polymorphisms, the risk for pre-eclampsia was significantly increased at 6.67 (p < 0.01), 5 (p < 0.01), 3.33 (p = 0.006), 2.72 (p = 0.04) and 7.8 (p < 0.01), respectively.
  
  
  CONCLUSIONS
  The results of our study confirm that, in pre-eclampsia, both maternal and newborn genetic variations implicated in blood pressure regulation are important.
authors: Department of Medical Biochemistry, Iuliu Hatieganu University of Medicine and Pharmacy Cluj-Napoca, Romania. luciamariaprocopciuc@yahoo.com
authors: Lucia Maria
authors: LM
authors: Procopciuc
authors: Gabriela
authors: G
authors: Caracostea
authors: Gabriela
authors: G
authors: Zaharie
authors: Mariana
authors: M
authors: Puscas
authors: Georgiana
authors: G
authors: Iordache
authors: Monica
authors: M
authors: Popa
authors: Doina
authors: D
authors: Colcear
authors: Ileana
authors: I
authors: Olteanu
authors: Florin
authors: F
authors: Stamatian
mesh_terms: D000328:Adult; D004587:Electrophoresis, Agar Gel; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D007231:Infant, Newborn; D009035:Mothers; D009154:Mutation / Q000235:genetics; D007703:Peptidyl-Dipeptidase A / Q000235:genetics; D011110:Polymorphism, Genetic; D011225:Pre-Eclampsia / Q000235:genetics* / Q000473:pathology; D011247:Pregnancy; D044140:Receptor, Angiotensin, Type 1 / Q000235:genetics; D012084:Renin-Angiotensin System / Q000235:genetics*; D012307:Risk Factors; D012383:Romania
chemical_list: D044140:Receptor, Angiotensin, Type 1; D007703:Peptidyl-Dipeptidase A