title: Transfer of stem cells carrying engineered chromosomes with XY clone laser system.
abstract: Current transgenic technologies for gene transfer into the germline of mammals cause a random integration of exogenous naked DNA into the host genome that can generate undesirable position effects as well as insertional mutations. The vectors used to generate transgenic animals are limited by the amount of foreign DNA they can carry. Mammalian artificial chromosomes have large DNA-carrying capacity and ability to replicate in parallel with, but without integration into, the host genome. Hence they are attractive vectors for transgenesis, cellular protein production, and gene therapy applications as well. ES cells mediated chromosome transfer by conventional blastocyst injection has a limitation in unpredictable germline transmission. The demonstrated protocol of laser-assisted microinjection of artificial chromosome containing ES cells into eight-cell mouse embryos protocol described here can solve the problem for faster production of germline transchromosomic mice.
authors: Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary. sinko@brc.hu
authors: Ildiko
authors: I
authors: Sinko
authors: Robert L
authors: RL
authors: Katona
mesh_terms: D000818:Animals; D001947:Breeding; D002460:Cell Line; D022221:Chromosomes, Artificial, Mammalian / Q000235:genetics*; D002999:Clone Cells / Q000166:cytology / Q000378:metabolism; D004624:Embryo Transfer; D005260:Female; D018014:Gene Transfer Techniques; D005818:Genetic Engineering; D007267:Injections; D007834:Lasers; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D009339:Needles; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000378:metabolism*; D014170:Transformation, Genetic