title: Gene cloning and characterization of novel antinociceptive peptide from the brain of the frog, Odorrana grahami.
abstract: Amphibian opiate peptides including dermorphins and deltorpins have been recently found only in the skin of South American frogs belonging to the subfamily Phyllomedusinae (Phyllomedusa, Agalychnis and Pachymedusa species). No opiate peptides have ever been identified from other amphibians or organs except skin. Here we report the purification and characterization of a novel antinociceptive peptide named odorranaopin from the homogenates of the frog brains, Odorrana grahami, which is also the first antinociceptive peptide found in Ranidae amphibian. Odorranaopin comprises 17 amino acid residues with the sequence of DYTIRTRLHQESSRKVL (Mr 2102 Da). The cDNA encoding odorranaopin was cloned from the frog brain cDNA library, and it was confirmed to be a specific gene. The odorranaopin precursor deduced is composed of 61 amino acid residues including the predicted signal peptide, acidic spacer peptide and mature odorranaopin positioned at the C-terminus. Odorranaopin could inhibit nociceptive responses induced by formalin and acetic acid. It also inhibited the contractile responses of ileum smooth muscle induced by bradykinin, implying that the antinociceptive activity of odorranaopin possibly results from its blockade on bradykinin or bradykinin receptor functions. Odorranaopin is the first antinociceptive peptide found in Ranidae amphibian.
authors: College of Life Sciences, Hebei Normal University, Shijiazhuang, Hebei, China.
authors: Wenlin
authors: W
authors: Chen
authors: Xuening
authors: X
authors: Yang
authors: Lingling
authors: L
authors: Chen
authors: Xiaolong
authors: X
authors: Yang
authors: Feifei
authors: F
authors: Feng
authors: Weiyu
authors: W
authors: He
authors: Jingze
authors: J
authors: Liu
authors: Haining
authors: H
authors: Yu
mesh_terms: D019342:Acetic Acid; D000595:Amino Acid Sequence; D029845:Amphibian Proteins / Q000235:genetics* / Q000494:pharmacology; D000700:Analgesics / Q000494:pharmacology; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D005260:Female; D015723:Gene Library; D006168:Guinea Pigs; D007082:Ileum / Q000187:drug effects / Q000502:physiology; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009119:Muscle Contraction / Q000187:drug effects; D010146:Pain / Q000139:chemically induced / Q000517:prevention & control; D010455:Peptides / Q000235:genetics* / Q000494:pharmacology; D011898:Ranidae / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D029845:Amphibian Proteins; D000700:Analgesics; D018076:DNA, Complementary; D010455:Peptides; C561577:odorranaopin peptide, Odorrana grahami; D019342:Acetic Acid