title: Label-free amperometric immunosensor for the detection of human serum chorionic gonadotropin based on nanoporous gold and graphene.
abstract: A label-free amperometric immunosensor for fast and sensitive assay of human serum chorionic gonadotropin (hCG) is presented. hCG was immobilized on nanoporous gold (NPG) foils and using hydroquinone (HQ) redox species as indicator. The variation of amperometric response to the concentration of hCG, the target antigen, was evaluated by cyclic voltammetry in phosphate-buffered solution. Taking advantage of dual-amplification effects of the NPG foils and graphene sheets (GSs), the immunosensor exhibited a specific response to hCG in the range of 0.5-40.00 ng ml(-1) with a detection limit of 0.034 ng ml(-1) under optimal conditions. It was demonstrated that our proposed method possesses good accuracy, acceptable precision, and reproducibility. The NPG showed a better sensitizing effect and stability as immobilization matrices.
authors: Shandong Provincial Key Laboratory of Fluorine Chemistry and Chemical Materials, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, People's Republic of China.
authors: Ru
authors: R
authors: Li
authors: Dan
authors: D
authors: Wu
authors: He
authors: H
authors: Li
authors: Caixia
authors: C
authors: Xu
authors: Huan
authors: H
authors: Wang
authors: Yanfang
authors: Y
authors: Zhao
authors: Yanyan
authors: Y
authors: Cai
authors: Qin
authors: Q
authors: Wei
authors: Bin
authors: B
authors: Du
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D006063:Chorionic Gonadotropin / Q000097:blood*; D055664:Electrochemical Techniques / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D006873:Hydroquinones / Q000737:chemistry; D007118:Immunoassay / Q000379:methods*; D058608:Nanopores / Q000648:ultrastructure*; D010084:Oxidation-Reduction; D016062:Porosity
chemical_list: D006063:Chorionic Gonadotropin; D006873:Hydroquinones; D006046:Gold; D006108:Graphite; C031927:hydroquinone