title: Imaging of EdU, an alkyne-tagged cell proliferation probe, by Raman microscopy.
abstract: Click-free imaging of the nuclear localization of an alkyne-tagged cell proliferation probe, EdU, in living cells was achieved for the first time by means of Raman microscopy. The alkyne tag shows an intense Raman band in a cellular Raman-silent region that is free of interference from endogenous molecules. This approach may eliminate the need for click reactions in the detection of alkyne-labeled molecules.
authors: Sodeoka Live Cell Chemistry Project, ERATO, Japan Science and Technology Agency, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan.
authors: Hiroyuki
authors: H
authors: Yamakoshi
authors: Kosuke
authors: K
authors: Dodo
authors: Masaya
authors: M
authors: Okada
authors: Jun
authors: J
authors: Ando
authors: Almar
authors: A
authors: Palonpon
authors: Katsumasa
authors: K
authors: Fujita
authors: Satoshi
authors: S
authors: Kawata
authors: Mikiko
authors: M
authors: Sodeoka
mesh_terms: D000480:Alkynes / Q000737:chemistry*; D049109:Cell Proliferation; D003857:Deoxyuridine / Q000031:analogs & derivatives* / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D008853:Microscopy / Q000379:methods*; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D000480:Alkynes; C031086:5-ethynyl-2'-deoxyuridine; D003857:Deoxyuridine