title: [Effects of Huoxue Zhitong decoration on transmembrane protein I and II induced apoptosis of signal transmission of the epidural scar tissue].
abstract: OBJECTIVE
  To study the effect of Houxue Zhitong decoration on the expression of the mitochondria-initiated apoptosis pathway and transmembrane protein I an II of the epidural scar tissue.
  
  
  METHODS
  A total of 60 New Zealand rabbits (weight: 2.5-3.0 kg) were randomly divided into four groups, sham operation group (D, n=15), control group (B, n=14), sodium hyaluronate group (C, n=15), Houxue Zhitong decoration group (D, n=15). Except for group A, 1.0 cm x 1.0 cm dura mater uncovered area laminectomy was performed at I (4) and I(5), covered with 0.5 ml sodium hyaluronate in group C, covered with same amount of saline in group B and D. First 2 weeks after operation, animals in group D were lavaged with 2.5 ml/kg Houxue Zhitong decoction by one a day for 14 days. Five rabbits of each group selected randomly were killed in the 2,4,8 weeks after laminectomy. The specimens were prepared for determination of the expression of Fas and FasL, at scar tissue by semiquantitative reserve transcription-polymerase chain reaction (RT-PCR). The degree of scar adhesion was evaluated according by Rydell method.
  
  
  RESULTS
  The adhesion area in group B was larger than of group C and D in the 4th and 8th week. However, the number of fibroblasts and inflammantory cells in group D was the least among the three groups in the 8th week. At 2, 4, 8 weeks after operation, as compared with group B the expression of Fas, FasL of group C and D were decreased (P < 0.05). Especially, at 2 weeks, as compared with group B the expression of this two cytokines of group D was significant decreased (P < 0.05), too. In group C and D the duramater adhesion was decreased (P < 0.05). The proliferation of fibroblast and fibroblastic function were inhibited (P < 0.05).
  
  
  CONCLUSIONS
  Huoxue Zhitong is able to down-regulated the expression of Fas, FasL, which inhibited the proliferation of fibroblast, the fibroblastic function and the synthesis of extracellular matrix in the epidural scar tissue. It is an effective way of reducing peridural scar formation and prevent the failed back surgery syndrome.
authors: Hangzhou Hospital of TCM, Hangzhou 310007, Zhejiang, China.
authors: Xi-ling
authors: XL
authors: Fan
authors: Hui
authors: H
authors: Zhou
authors: Zhi-min
authors: ZM
authors: Xia
authors: Hai-xin
authors: HX
authors: Niu
authors: Jun-jun
authors: JJ
authors: Luo
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002921:Cicatrix / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004824:Epidural Space / Q000473:pathology; D053222:Fas Ligand Protein / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D011817:Rabbits; D015398:Signal Transduction / Q000187:drug effects*; D019014:fas Receptor / Q000235:genetics / Q000378:metabolism*
chemical_list: D004365:Drugs, Chinese Herbal; D053222:Fas Ligand Protein; D019014:fas Receptor