title: Secretome analysis of rat adipose tissues shows location-specific roles for each depot type.
abstract: Obesity prevalence is reaching pandemic proportions becoming a major public health threat for many industrialized nations. It is especially worrying as it causes a higher risk of premature death due to associated diseases such as type 2 diabetes, cardiovascular disease, and some cancers. Current evidence shows biological and genetic differences between adipose tissues depending on its anatomical location. Particularly, upper body/visceral fat distribution in obesity is closely linked to metabolic complications. In this report, we characterize for the first time the secretome of rat adipose tissue explants from different anatomical localizations and its differential analysis. Visceral, subcutaneous, and gonadal fat specific secretomes and differentially secreted proteins among the three fat depots were analyzed by 2-DE and MS. Reference maps for location-specific adipose tissue secretomes are shown and the 45 most significant differences are listed. Identified proteins include classical adipokines and novel secreted proteins. Interestingly, our results show that the type of proteins and their role in different biological processes diverge significantly when comparing the set of proteins identified from visceral, subcutaneous and gonadal fat explants. This study emphasizes and supports the differential role of adipose tissue in accordance to its anatomical localization.
authors: Grupo Obesidómica, Laboratorio de Endocrinología Molecular y Celular, Instituto de Investigación Sanitaria de Santiago de Compostela, Complexo Hospitalario Universitario de Santiago (CHUS/SERGAS), Santiago de Compostela, Spain.
authors: Arturo
authors: A
authors: Roca-Rivada
authors: Jana
authors: J
authors: Alonso
authors: Omar
authors: O
authors: Al-Massadi
authors: Cecilia
authors: C
authors: Castelao
authors: Juan Ramón
authors: JR
authors: Peinado
authors: Luisa María
authors: LM
authors: Seoane
authors: Felipe F
authors: FF
authors: Casanueva
authors: María
authors: M
authors: Pardo
mesh_terms: D054392:Adipokines / Q000378:metabolism*; D000273:Adipose Tissue / Q000033:anatomy & histology / Q000378:metabolism*; D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling; D006066:Gonads; D050152:Intra-Abdominal Fat / Q000378:metabolism; D008297:Male; D009765:Obesity / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D050151:Subcutaneous Fat / Q000378:metabolism
chemical_list: D054392:Adipokines