title: [Production of pertussis toxin by Bordetella pertussis strains isolated from patients with whooping cough].
abstract: OBJECTIVE
  To assess level of pertussin toxin (PT) production by vaccine strains of Bordetella pertussis and strains isolated from patients with whooping cough.
  
  
  METHODS
  Concentration of PT in supernatants of microbial cultures of 3 vaccine strains and 25 strains of B. pertussis isolated from patients with pertussis in 2001 - 2005 was measured with enzyme immunoassay using gamma-globulin fractions of rabbit antiserum to PT as immunosorbent or included in peroxidase conjugates.
  
  
  RESULTS
  Level of PT production by strains isolated from infected persons varied from 3 +/- 0.5 to 64.8 +/- 12.2 ng/MFU/ml: in 9 strains--from 3 +/- 0.5 to 9.4 +/- 2.1 ng/MFU/ml, in 7--10.5 +/- 1.8 to 18.4 +/- 2.6 ng/MFU/ml, and in 9--23.6 +/- 4.5 to 64.8 +/- 12.2 ng/MFU/ml.
  
  
  CONCLUSIONS
  B. pertussis strains isolated from patients were heterogeneous on level of PT production. Difference in expression of PT between strains were as high as 20-fold. Conditionally low, moderate and high levels of PT production had 9 (36%), 7 (28%), and 9 (36%) of 25 studied strains. Three vaccine strains had levels of toxin production similar to recently isolated strains with moderate level of its production.
authors: E M
authors: EM
authors: Zaĭtsev
authors: N U
authors: NU
authors: Mertsalova
authors: A S
authors: AS
authors: Shinkarev
authors: I K
authors: IK
authors: Mazurova
authors: N S
authors: NS
authors: Zakharova
mesh_terms: D000818:Animals; D001886:Bordetella pertussis / Q000201:enzymology* / Q000302:isolation & purification* / Q000472:pathogenicity; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D008297:Male; D037342:Pertussis Toxin / Q000096:biosynthesis*; D011817:Rabbits; D014917:Whooping Cough / Q000201:enzymology* / Q000382:microbiology
chemical_list: D037342:Pertussis Toxin