title: Monitoring CD4 in whole blood with an opto-fluidic detector based on spatially modulated fluorescence emission.
abstract: We describe a new optical detection technique, termed "spatially modulated fluorescence emission," that delivers high signal-to-noise discrimination without precision optics to enable a flow cytometer that can combine high performance, robustness, compactness, low cost, and ease of use. The detection technique is demonstrated with measurements of absolute CD4+ and percentage CD4 counts in human blood. Benchmarking against a commonly used commercial instrument for this test yields excellent agreement for both absolute CD4 and percentage CD4.
authors: Palo Alto Research Center, Electronic Materials and Devices Laboratory, Palo Alto, CA 94304, USA. peter.kiesel@parc.com
authors: Peter
authors: P
authors: Kiesel
authors: Markus
authors: M
authors: Beck
authors: Noble
authors: N
authors: Johnson
mesh_terms: D018791:CD4 Lymphocyte Count; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology*; D005434:Flow Cytometry / Q000295:instrumentation* / Q000379:methods*; D005453:Fluorescence; D006801:Humans; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods