title: Reovirus inhibits poliovirus replication upon superinfection.
abstract: OBJECTIVE
  Viral interference has been demonstrated in different systems, such as the effect of enterovirus infection on live-attenuated oral polio vaccine. In this study, the effect of reovirus which could exist in the human intestinal tract on poliovirus vaccine strains was investigated and could be an important factor to consider in oral polio vaccination.
  
  
  METHODS
  Cells were infected with reovirus, then superinfected with poliovirus. The amount of viral yields was measured by the TCID(50) and plaque assay methods. Polioviral RNA synthesis was studied by real-time RT-PCR and the viral RNA load was calculated. Viral protein synthesis was determined using the techniques of immunoflourescent staining and PAGE followed by the immunoblotting experiment.
  
  
  RESULTS
  Poliovirus superinfection of reovirus-infected cells resulted in inhibition of poliovirus replication. It was found that the inhibitory effect of reovirus was after establishment of its infection (2 h postinfection). There was no competition between the two viruses for cell attachment but poliovirus RNA and protein synthesis were inhibited.
  
  
  CONCLUSIONS
  Infection of cells with reovirus could interfere with the growth of poliovirus upon superinfection. This phenomenon could be important to consider when using attenuated poliovirus vaccine.
authors: Department of Virology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
authors: Angila Ataei
authors: AA
authors: Pirkooh
authors: Mahmoud Shamsi
authors: MS
authors: Shahrabadi
authors: Hamidreza
authors: H
authors: Monavari
authors: Abbas
authors: A
authors: Shafiei
mesh_terms: D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D051379:Mice; D008856:Microscopy, Fluorescence; D017955:Poliovirus / Q000254:growth & development / Q000502:physiology*; D012367:RNA, Viral / Q000032:analysis; D011817:Rabbits; D060888:Real-Time Polymerase Chain Reaction; D012087:Reoviridae / Q000254:growth & development / Q000502:physiology*; D014762:Viral Interference; D019562:Viral Load; D010948:Viral Plaque Assay; D014764:Viral Proteins / Q000032:analysis
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins