title: Preparation of dexamethasone-based cationic liposome and its application to gene delivery in vitro.
abstract: In this study, dexamethasone was conjugated to PAMAM dendrimer (generation 0) and its gene transfection efficiency was investigated. To make a liposomal solution for gene delivery, DOPE was used as a fusogenic helper lipid. In gel retardation assay, PAMAM-dexamethasone conjugate (PAM-Dex)/DOPE liposome/DNA complex was completely retarded at 8:1 N/P (nitrogen/phosphate) ratio. The physicochemical characteristics are studied by measuring the average size distribution and zeta-potential values of the complexes. In vitro transfection assay showed that the PAM-Dex/DOPE liposome/DNA complex displayed higher gene delivery efficiency compared to PAMAM/DNA complex. In addition, PAM-Dex/DOPE liposome showed the lowest toxicity compared to PAMAM, PEI 25 kD and Lipofectamine. These results indicate that PAM-Dex/DOPE liposome has a potential to be used as an efficient gene carrier for gene therapy.
authors: Department of Biochemistry, Chungnam National University, Daejeon 305-764, Korea.
authors: Tae-Hun
authors: TH
authors: Kim
authors: Gwang Sig
authors: GS
authors: Yu
authors: Hye
authors: H
authors: Choi
authors: Young Jung
authors: YJ
authors: Shim
authors: Minhyung
authors: M
authors: Lee
authors: Joon Sig
authors: JS
authors: Choi
mesh_terms: D002412:Cations; D002460:Cell Line; D050091:Dendrimers / Q000737:chemistry / Q000633:toxicity; D003907:Dexamethasone / Q000737:chemistry* / Q000633:toxicity; D004337:Drug Carriers / Q000737:chemistry / Q000633:toxicity; D006801:Humans; D066298:In Vitro Techniques; D008081:Liposomes / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity; D036103:Nanotechnology; D010316:Particle Size; D010714:Phosphatidylethanolamines / Q000737:chemistry; D014162:Transfection / Q000379:methods*
chemical_list: C410262:1,2-dioleoyl-glycero-3-phosphatidyl ethanolamine; D002412:Cations; D050091:Dendrimers; D004337:Drug Carriers; D008081:Liposomes; C104700:PAMAM Starburst; D010714:Phosphatidylethanolamines; D003907:Dexamethasone