title: A subcellular localization compendium of hydrogen peroxide-induced proteins.
abstract: The signal transduction mechanisms of the oxidative stress response in plants remain largely unexplored. Previously, increased levels of cellular hydrogen peroxide (H(2)O(2)) had been shown to drastically affect the plant transcriptome. Genome-wide transcriptome analyses allowed us to build a comprehensive inventory of H(2)O(2)-induced genes in plants. Here, the primary objective was to determine the subcellular localization of these genes and to assess potential trafficking during oxidative stress. After high-throughput cloning in Gateway-derived vectors, the subcellular localization of 49 proteins fused to the green fluorescent protein (GFP) was identified in a transient assay in tobacco (Nicotiana benthamiana) by means of agro-infiltration and confirmed for a selection of genes in transgenic Arabidopsis thaliana plants. Whereas eight of the GFP-tagged proteins are exclusively localized in the nucleus, 23 reside both in the nucleus and cytosol, in which several classes of known transcription factors and proteins of unknown function can be recognized. In this study, the mapping of the subcellular localization of H(2)O(2) -induced proteins paves the way for future research to unravel the H(2)O(2) responses in plants. Furthermore, the effect of increased H(2)O(2) levels on the subcellular localization of a subset of proteins was assessed.
authors: Department of Plant Systems Biology, VIB, 9052 Gent, Belgium.
authors: Annelies
authors: A
authors: Inzé
authors: Sandy
authors: S
authors: Vanderauwera
authors: Frank A
authors: FA
authors: Hoeberichts
authors: Michaël
authors: M
authors: Vandorpe
authors: Tim
authors: T
authors: Van Gaever
authors: Frank
authors: F
authors: Van Breusegem
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000502:physiology* / Q000648:ultrastructure; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D003001:Cloning, Molecular; D016000:Cluster Analysis; D003600:Cytosol / Q000378:metabolism; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D049452:Green Fluorescent Proteins; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D020411:Oligonucleotide Array Sequence Analysis; D018384:Oxidative Stress; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D021381:Protein Transport / Q000187:drug effects; D011993:Recombinant Fusion Proteins; D015398:Signal Transduction / Q000187:drug effects*; D013312:Stress, Physiological; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism; D059467:Transcriptome / Q000187:drug effects
chemical_list: D029681:Arabidopsis Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D006861:Hydrogen Peroxide