title: Ordered assembly of murine leukemia virus capsid protein on lipid nanotubes directs specific binding by the restriction factor, Fv1.
abstract: The restriction factor Fv1 confers resistance to murine leukemia virus (MLV), blocking progression of the viral life cycle after reverse transcription, but before integration into the host chromosome. It is known that the specificity of restriction is determined by both the restriction factor and the viral capsid (CA), but a direct interaction between Fv1 and MLV CA has not yet been demonstrated. With the development of a previously unexplored method for in vitro polymerization of MLV CA, it has now been possible to display a binding interaction between Fv1 and MLV CA. C-terminally His-tagged CA molecules were assembled on Ni-chelating lipid nanotubes, and analysis by electron microscopy revealed the formation of a regular lattice. Comparison of binding data with existing restriction data confirmed the specificity of the binding interaction, with multiple positions of both Fv1 and CA shown to influence binding specificity.
authors: Division of Virology, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, United Kingdom.
authors: Laura
authors: L
authors: Hilditch
authors: Rishi
authors: R
authors: Matadeen
authors: David C
authors: DC
authors: Goldstone
authors: Peter B
authors: PB
authors: Rosenthal
authors: Ian A
authors: IA
authors: Taylor
authors: Jonathan P
authors: JP
authors: Stoye
mesh_terms: D000818:Animals; D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism*; D017931:DNA Primers / Q000235:genetics; D007091:Image Processing, Computer-Assisted; D009052:Leukemia Virus, Murine / Q000378:metabolism*; D050356:Lipid Metabolism; D051379:Mice; D008854:Microscopy, Electron; D016296:Mutagenesis; D043942:Nanotubes / Q000648:ultrastructure / Q000821:virology*; D010957:Plasmids / Q000235:genetics; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*
chemical_list: D036022:Capsid Proteins; D017931:DNA Primers; C103189:Fv1 protein, mouse; D011506:Proteins