title: [Effect of JNK pathway in apoptosis of PC12 cell by MPP+].
abstract: OBJECTIVE
  To study the toxicity and its mechanisms of 1-methyl-4-phenylpyridinium ion (MPP+) on pheochromocytoma PC12 cells.
  
  
  METHODS
  PC12 cells were cultured in vitro, and poisoned by 100, 300, 500 micromol/L MPP+. Western blot was performed to determine the level of phosphorylated c-Jun-N-terminal kinase/stress activated protein kinases (JNK/SAPK). The cells were pretreated with SP600125, an inhibitor of JNK pathway, and then the number of apoptotic cells were counted by using TUNEL stain, observing its influence on cell apoptosis seduced by MPP+.
  
  
  RESULTS
  MPP+ poisoning can cause the increase of phosphorylation level of JNK1/2 cells. The usage of JNK pathway inhibitor SP600125 can inhibit the PC12 cell apoptosis seduced by MPP+.
  
  
  CONCLUSIONS
  Activation of JNK pathway may be the important molecular mechanism of PC12 cell apoptosis seduced by MPP+ and of producing dopaminergic neurotoxicity.
authors: Department of Occupational and Environmental Health, Fourth Military Medical University, Xi'an 710032, China.
authors: Gang
authors: G
authors: Zheng
authors: Wenjing
authors: W
authors: Luo
authors: Xueping
authors: X
authors: Zhang
authors: Fang
authors: F
authors: Zhao
authors: Jiye
authors: J
authors: Wang
authors: Yaoming
authors: Y
authors: Chen
authors: Jingyuan
authors: J
authors: Chen
mesh_terms: D015655:1-Methyl-4-phenylpyridinium / Q000633:toxicity*; D000818:Animals; D000873:Anthracenes / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D016716:PC12 Cells; D051381:Rats
chemical_list: D000873:Anthracenes; C432165:pyrazolanthrone; D015655:1-Methyl-4-phenylpyridinium