title: The expression of the pro-apoptotic gene Air is inducible in human pancreatic adenocarcinoma cells.
abstract: We previously identified apoptosis-induced regulator (air) as a pro-apoptotic transcript whose expression was repressed by NF-κB/Rel activity in the human leukemia cell line Jurkat (Turco, Lamberti, Bisogni, Romano, Petrella, Ammirante, Rosati, d'Avenia, Arra, Spugnini, Venuta, 2007, Leukemia 21:2557-2559). In this paper, we report that air sequence is detectable by Southern blot in human healthy donors (HHD) leukocytes and in two pancreatic adenocarcinoma cell lines (AsPC-1 and PANC-1), providing the first definitive evidence of air gene presence in human genome. In addition, we demonstrate that air expression is induced in the tumor cell lines by a naturally occurring ROS-inducing compound, phenethyl isothiocyanate (PEITC), a potential dietary cancer chemopreventive agent. Since PEITC inhibits NF-κB activation, air induction by this agent is consistent with the suppressive effect of NF-κB on air expression. This finding contributes a new clue to the role of NF-κB in regulating oxidative stress-induced pro-apoptotic genes and identifies a novel potential tool for enhancing pancreas cancer response to treatment.
authors: Department of Pharmaceutical and Biomedical Sciences, University of Salerno, Salerno, Italy.
authors: Morena
authors: M
authors: d'Avenia
authors: Alessandra
authors: A
authors: Rosati
authors: Maria Antonietta
authors: MA
authors: Belisario
authors: Mario
authors: M
authors: Torino
authors: Gaetano
authors: G
authors: Torino
authors: Maria Caterina
authors: MC
authors: Turco
authors: Maria
authors: M
authors: Pascale
mesh_terms: D017209:Apoptosis / Q000187:drug effects / Q000235:genetics*; D051017:Apoptosis Regulatory Proteins / Q000235:genetics* / Q000378:metabolism; D015139:Blotting, Southern; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D015894:Genome, Human / Q000235:genetics; D006801:Humans; D017879:Isothiocyanates / Q000494:pharmacology; D016328:NF-kappa B / Q000378:metabolism; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000473:pathology*; D021381:Protein Transport / Q000187:drug effects
chemical_list: D051017:Apoptosis Regulatory Proteins; D017879:Isothiocyanates; D016328:NF-kappa B; C525108:apoptosis-induced regulator protein; C058305:phenethyl isothiocyanate; D002945:Cisplatin