title: Telomere G-overhang length measurement method 1: the DSN method.
abstract: Telomeres terminate in 3' single-stranded G-overhangs that function in telomere end protection and telomerase action. An accurate measurement of overhang length is challenging due to the presence of many kilobases of double-stranded telomere DNA. Here, a simple method is described that utilizes duplex-specific nuclease (DSN) to digest all genomic DNA including telomeres, leaving the single-stranded overhangs intact. The telomere single-strand G-rich overhang length can then be determined by Southern blot-based assays.
authors: Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX, USA.
authors: Yong
authors: Y
authors: Zhao
authors: Jerry W
authors: JW
authors: Shay
authors: Woodring E
authors: WE
authors: Wright
mesh_terms: D001483:Base Sequence; D004277:DNA, Single-Stranded / Q000235:genetics / Q000378:metabolism; D003851:Deoxyribonucleases / Q000378:metabolism*; D006147:Guanine / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D016615:Telomere / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
chemical_list: D004277:DNA, Single-Stranded; D006147:Guanine; D003851:Deoxyribonucleases