title: Isolation of Fusarium sp. from a claw of a dog with onychomycosis.
abstract: An 8-year-old male Golden Retriever had lameness and claw abnormality in the second digit of the left forelimb. Radiography revealed osteomyelitis in the distal phalanx bone of the affected limb. Microscopic examination of the claw revealed numerous hyphae in the claw matrix. Fungal DNA fragments coding the ribosomal internal transcribed spacer region (ITS) were detected from the claw matrix as well as fungal colonies of the clinical isolates by PCR. Nucleotide sequencing revealed that the amplicons shared > 99% homology with Fusarium sp. Therapy including oral itraconazole resulted in regrowth of a new claw, in which no hyphae were detected. To the authors' knowledge, this is the first case report of canine onychomycosis in which Fusarium sp. was isolated from the affected claw.
authors: Laboratory of Veterinary Internal Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, Japan.
authors: Kazuko
authors: K
authors: Namitome
authors: Rui
authors: R
authors: Kano
authors: Maiko
authors: M
authors: Sekiguchi
authors: Toshiroh
authors: T
authors: Iwasaki
authors: Takashi
authors: T
authors: Kaneshima
authors: Koji
authors: K
authors: Nishifuji
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000627:therapeutic use; D001483:Base Sequence; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D004283:Dog Diseases / Q000188:drug therapy / Q000382:microbiology* / Q000601:surgery; D004285:Dogs; D005533:Foot Dermatoses / Q000188:drug therapy / Q000382:microbiology / Q000601:surgery / Q000662:veterinary*; D060585:Fusariosis / Q000188:drug therapy / Q000382:microbiology / Q000601:surgery / Q000662:veterinary*; D005670:Fusarium / Q000235:genetics / Q000302:isolation & purification*; D006651:Histocytochemistry / Q000662:veterinary; D006724:Hoof and Claw / Q000382:microbiology* / Q000601:surgery; D017964:Itraconazole / Q000627:therapeutic use; D008297:Male; D008969:Molecular Sequence Data; D014009:Onychomycosis / Q000188:drug therapy / Q000382:microbiology / Q000601:surgery / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D000935:Antifungal Agents; D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D017964:Itraconazole