title: [Influence of Streptococcus mutans luxS gene on polysaccharide matrix metabolism].
abstract: OBJECTIVE
  To investigate the effect of Streptococcus mutans luxS gene on polysaccharide matrix metabolism.
  
  
  METHODS
  Based on the immobilization of magnetic beads by adherent cells,an assay of biofilm quantitative analysis was developed for the kinetic quantification of biofilm formation. S.mutans luxS gene mutant strain and wild-type strain were compared for their ability of utilizing exogenous carbohydrate to form extracellular polysaccharide matrix. SPSS 10.0 software package was used for statistical analysis. Dunnet t two-side test of one factor analysis of variance was performed.
  
  
  RESULTS
  Both luxS mutant strain and wild-type strain could use exogenous carbohydrate to form polysaccharide matrix.With 1% sucrose added ,both strains completed their biofilm formation within one hour.When adding 1% glucose,these strains also accelerated the formation of biofilm,and this was more significant in the mutant strain.
  
  
  CONCLUSIONS
  The luxS gene of S. mutans can regulate its extracellular polysaccharide matrix metabolism. Moreover, the regulation of this gene on biofilm formation is more probably via polysaccharide matrix pathway. Supported by National Natural Science Foundation of China(30872886), Research Fund of Science and Technology Commission of Shanghai Municipality(08DZ2271100),Shanghai Leading Academic Discipline Project(S30206) and Youth Phosphor Program of Science and Technology Commission of Shanghai Municipality (09QA1403700).
authors: Department of Endodontics, Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 200011, China. qtdc1010@163.com
authors: Qian
authors: Q
authors: Wang
authors: Zi-sheng
authors: ZS
authors: Tang
authors: Rui
authors: R
authors: Ma
authors: Zheng
authors: Z
authors: Liu
authors: Cai-lian
authors: CL
authors: Zhu
authors: Zheng-wei
authors: ZW
authors: Huang
mesh_terms: D001426:Bacterial Proteins; D018441:Biofilms; D013437:Carbon-Sulfur Lyases; D006801:Humans; D011134:Polysaccharides; D013295:Streptococcus mutans
chemical_list: D001426:Bacterial Proteins; D011134:Polysaccharides; D013437:Carbon-Sulfur Lyases