title: The spatio-temporal expression of ProSAP/shank family members and their interaction partner LAPSER1 during Xenopus laevis development.
abstract: Members of the ProSAP/Shank family are important scaffolding proteins of the postsynaptic density (PSD). We investigated for the first time the expression of the three family members named Shank1, ProSAP1/Shank2, and ProSAP2/Shank3 during Xenopus laevis development. Shank1 is expressed in the neural tube, the retina, and the cranial ganglions. In contrast, ProSAP1/Shank2 transcripts could be visualized in the otic vesicle, the pronephros, the liver, the neural tube, and the retina. ProSAP2/Shank3 could be detected in the cardiovascular network, the neural tube, the pronephros, and the retina. Furthermore, we showed that LAPSER1 interacts with all three ProSAP/Shank family members in Xenopus embryos and co-localizes with ProSAP/Shank in a cell-based assay. In Xenopus, LAPSER1 is expressed in somites, brain, proctodeum, pronephros, and in some cranial ganglions. Thus, we suggest that members of the ProSAP/Shank family and LAPSER1 not only play a role in PSD formation and plasticity, but also during embryonic development.
authors: Institute for Biochemistry and Molecular Biology, Ulm University, Ulm, Germany.
authors: Susanne
authors: S
authors: Gessert
authors: Michael J
authors: MJ
authors: Schmeisser
authors: Si
authors: S
authors: Tao
authors: Tobias M
authors: TM
authors: Boeckers
authors: Michael
authors: M
authors: Kühl
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism; D000818:Animals; D030801:Animals, Genetically Modified; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D004625:Embryo, Nonmammalian; D047108:Embryonic Development / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D005810:Multigene Family; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D057907:Post-Synaptic Density / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D013569:Synapses / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D014018:Tissue Distribution; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism; D029867:Xenopus Proteins / Q000235:genetics* / Q000378:metabolism*; D014982:Xenopus laevis / Q000196:embryology* / Q000235:genetics* / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D018797:Cell Cycle Proteins; C561479:LZTS2 protein, Xenopus; D009419:Nerve Tissue Proteins; C561477:Shank2 protein, Xenopus; C561478:Shank3 protein, Xenopus; D025521:Tumor Suppressor Proteins; D029867:Xenopus Proteins