title: Molecular characterization of Indian rabies virus isolates by partial sequencing of nucleoprotein (N) and phosphoprotein (P) genes.
abstract: Rabies is endemic and an important zoonosis in India. There are very few reports available on molecular epidemiology of rabies virus of Indian origin. In this study to know the dynamics of rabies virus, a total of 41 rabies positive brain samples from dogs, cats, domestic animals, wildlife, and humans from 11 states were subjected to RT-PCR amplification of N gene between nucleotide N521-N1262 (742 bp) and P gene between nucleotide P239-P750 (512 bp). The N gene could be amplified from 30, while P gene from 41 samples, using specific sets of primers. The N gene-based phylogenetic analysis indicated that all Indian virus isolates are genetically closely related with a single cluster under arctic/arctic-like viruses. However, two distinct clusters were realized in P gene-based phylogeny viz., Rabies virus isolates of Punjab and Rabies virus isolates of remaining parts of India (other than Punjab). All the Indian rabies virus isolates were closely related to geography (>95% homology), but not to host species.
authors: Division of Animal Health, Central Institute for Research on Goats, Makhdoom, Farah, Mathura, 281122, UP, India.
authors: G B Manjunatha
authors: GB
authors: Reddy
authors: R
authors: R
authors: Singh
authors: R P
authors: RP
authors: Singh
authors: K P
authors: KP
authors: Singh
authors: P K
authors: PK
authors: Gupta
authors: Anita
authors: A
authors: Mahadevan
authors: Anita
authors: A
authors: Desai
authors: S K
authors: SK
authors: Shankar
authors: M A
authors: MA
authors: Ramakrishnan
authors: Rishendra
authors: R
authors: Verma
mesh_terms: D000818:Animals; D000835:Animals, Wild; D002415:Cats; D016000:Cluster Analysis; D004285:Dogs; D005838:Genotype; D006801:Humans; D007194:India / Q000453:epidemiology; D018832:Molecular Chaperones; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D019590:Nucleocapsid Proteins / Q000235:genetics*; D057805:Pets; D010750:Phosphoproteins / Q000235:genetics*; D058974:Phylogeography; D012367:RNA, Viral / Q000235:genetics; D011818:Rabies / Q000453:epidemiology* / Q000662:veterinary* / Q000821:virology; D011820:Rabies virus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D015678:Viral Structural Proteins / Q000235:genetics*
chemical_list: D018832:Molecular Chaperones; D019590:Nucleocapsid Proteins; C103961:P phosphoprotein, Rabies virus; D010750:Phosphoproteins; D012367:RNA, Viral; D015678:Viral Structural Proteins; C099601:nucleocapsid protein, Rabies virus