title: Long-term supplementation with resveratrol alleviates oxidative stress but does not attenuate sarcopenia in aged mice.
abstract: This study analyzed the capacity of resveratrol, a naturally occurring polyphenol, to reduce aging-induced oxidative stress and protect against sarcopenia. Middle-aged (18 months) C57/BL6 mice were randomly assigned to receive either a control diet or a diet supplemented with 0.05% trans-resveratrol for 10 months. Young (6 months) and middle-aged (18 months) mice were used as controls. Resveratrol supplementation did not reduce the aging-associated loss of muscle mass or improve maximal isometric force production, but it appeared to preserve fast-twitch fiber contractile function. Resveratrol supplementation did not improve mitochondrial content, the subcellular localization of cytochrome c protein content, or PGC1 protein content. Resveratrol increased manganese superoxide dismutase (MnSOD), reduced hydrogen peroxide(,) and lipid peroxidation levels in muscle samples, but it was unable to significantly reduce protein carbonyl levels. The data suggest that resveratrol has a protective effect against aging-induced oxidative stress in skeletal muscle, likely through the upregulation of MnSOD activity, but sarcopenia was not attenuated by resveratrol.
authors: Division of Exercise Physiology, School of Medicine, Robert C. Byrd Health Sciences Center, West Virginia University, Morgantown, USA. salway@hsc.wvu.edu
authors: Janna R
authors: JR
authors: Jackson
authors: Michael J
authors: MJ
authors: Ryan
authors: Stephen E
authors: SE
authors: Alway
mesh_terms: D000375:Aging / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage; D019587:Dietary Supplements; D004195:Disease Models, Animal; D005500:Follow-Up Studies; D015151:Immunoblotting; D007537:Isometric Contraction / Q000187:drug effects; D051379:Mice; D008810:Mice, Inbred C57BL; D008931:Mitochondria, Muscle / Q000187:drug effects / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D018384:Oxidative Stress / Q000187:drug effects*; D000077185:Resveratrol; D012264:Ribonucleotide Reductases / Q000037:antagonists & inhibitors; D055948:Sarcopenia / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D013267:Stilbenes / Q000008:administration & dosage*; D013997:Time Factors
chemical_list: D000975:Antioxidants; D013267:Stilbenes; D012264:Ribonucleotide Reductases; D000077185:Resveratrol