title: Structural modifications occurring in lipid A of Bordetella bronchiseptica clinical isolates as demonstrated by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
abstract: Bordetella bronchiseptica is a respiratory pathogen in mammal species and its cell surface lipopolysaccharide-endotoxin is a potent virulence factor. In order to better characterize the endotoxin structure to virulence relationships, we studied the lipid A structures of B. bronchiseptica isolates from human and rabbit origins as a function of their virulence phases. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) has been widely used for the structural characterization of bacterial endotoxins and their lipid A moieties. This method combined with chemical analytical methods proved to be essential for the characterization of small samples and discrete but essential structural modifications. The occurrence of palmitate (C(16)) in the B. bronchiseptica lipid A structures is shown for the first time at two sites. Their presence was also demonstrated for the first time in correlation with the virulence phase of B. bronchiseptica clinical isolates. The recently identified glucosamine modifications of Bordetella lipids A are also reported in these isolates.
authors: Endotoxines, Structures et Activités, UMR 8621, GDR 3048, du CNRS, Institut de Génétique et Microbiologie, Université de Paris-Sud, 91405, Orsay, France.
authors: Soorej M
authors: SM
authors: Basheer
authors: Nicole
authors: N
authors: Guiso
authors: Alina
authors: A
authors: Tirsoaga
authors: Martine
authors: M
authors: Caroff
authors: Alexey
authors: A
authors: Novikov
mesh_terms: D000818:Animals; D001885:Bordetella Infections / Q000382:microbiology; D016950:Bordetella bronchiseptica / Q000737:chemistry* / Q000302:isolation & purification; D005944:Glucosamine / Q000737:chemistry; D006801:Humans; D008050:Lipid A / Q000737:chemistry*; D015394:Molecular Structure; D019308:Palmitic Acid / Q000737:chemistry; D011817:Rabbits; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D008050:Lipid A; D019308:Palmitic Acid; D005944:Glucosamine