title: [Systems biology approach to the functional role of enzymatic modification of bacterial ribosome].
abstract: In this work we describe methodology for studying the role of bacterial ribosome modification in the regulation of gene expression. Ribosomal components modification influences translation efficiencies of certain mRNAs. Proteome analysis allows us to identify cellular protein composition change caused by ribosome modification gene knockout. Particular stage of gene expression responsible for certain protein concentration change could be found using reporter constructs. After identification of mRNA species, whose translation is influenced by ribosome modification we can determine exact mRNA region responsible for the observed changes. The developed methodology can be applied for studying other translational control mechanisms.
authors: P V
authors: PV
authors: Sergiev
authors: I A
authors: IA
authors: Osterman
authors: I V
authors: IV
authors: Prokhorova
authors: M V
authors: MV
authors: Nesterchuk
authors: O V
authors: OV
authors: Sergeeva
authors: A Ia
authors: AIa
authors: Golovina
authors: I A
authors: IA
authors: Demina
authors: M A
authors: MA
authors: Galiamina
authors: M V
authors: MV
authors: Serebriakova
authors: O A
authors: OA
authors: Dontsova
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D017930:Genes, Reporter; D015151:Immunoblotting; D007763:Lac Operon; D008156:Luciferases / Q000235:genetics; D008780:Methyltransferases / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D012323:RNA Processing, Post-Transcriptional; D012329:RNA, Bacterial / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D012335:RNA, Ribosomal / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D020543:Proteome; D012329:RNA, Bacterial; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D008156:Luciferases; D008780:Methyltransferases; D001616:beta-Galactosidase