title: A mitochondrial-targeted two-photon probe for zinc ion.
abstract: We report a two-photon probe (SZn-Mito) for mitochondrial zinc ions ([Zn2+]m). This probe shows a 7-fold enhancement of two-photon-excited fluorescence in response to Zn2+ with a dissociation constant (Kd(TP)) of 3.1 ± 0.1 nM and pH insensitivity in the biologically relevant range, allowing the detection of [Zn2+]m in a rat hippocampal slice at a depth of 100−200 μm without interference from other metal ions through the use of two-photon microscopy.
authors: Molecular Science and Technology Research Center, Ajou University, Suwon 443-749, Korea.
authors: Goutam
authors: G
authors: Masanta
authors: Chang Su
authors: CS
authors: Lim
authors: Hyung Joong
authors: HJ
authors: Kim
authors: Ji Hee
authors: JH
authors: Han
authors: Hwan Myung
authors: HM
authors: Kim
authors: Bong Rae
authors: BR
authors: Cho
mesh_terms: D000818:Animals; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods; D008928:Mitochondria / Q000737:chemistry*; D051381:Rats; D012680:Sensitivity and Specificity; D015032:Zinc / Q000032:analysis*
chemical_list: D005456:Fluorescent Dyes; D015032:Zinc