title: Covalent immobilization of nisin on multi-walled carbon nanotubes: superior antimicrobial and anti-biofilm properties.
abstract: Despite unique and useful properties of multi-walled carbon nanotubes (MWNTs) such as high strength and a low synthesis cost, their weak antimicrobial property hampers their use as an antimicrobial material. Herein, we demonstrate that the immobilization of nisin, a natural and inexpensive antimicrobial peptide, with poly(ethylene glycol) (PEG(1000)) as a linker significantly enhanced the antimicrobial and anti-biofilm properties of MWNTs. The MWNT-nisin composite showed up to 7-fold higher antimicrobial property than pristine MWNTs against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis. Moreover, the MWNT-nisin composite had a dramatically improved capability to prevent biofilm formation both on a deposited film and in suspension. In particular, the MWNT-nisin deposit film exhibited a 100-fold higher anti-biofilm property than the MWNT deposit film. Further, it has been shown that PEG and nisin are covalently attached to MWNTs with excellent stability against leaching. We envision that our novel MWNT-nisin composite can serve as an effective and economical antimicrobial material.
authors: Division of Chemical and Biomolecular Engineering, School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, 637459, Singapore.
authors: Xiaobao
authors: X
authors: Qi
authors: Gunawan
authors: G
authors: Poernomo
authors: Kean
authors: K
authors: Wang
authors: Yuan
authors: Y
authors: Chen
authors: Mary B
authors: MB
authors: Chan-Park
authors: Rong
authors: R
authors: Xu
authors: Matthew Wook
authors: MW
authors: Chang
mesh_terms: D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000494:pharmacology; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development*; D008422:Materials Testing; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D009561:Nisin / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D037742:Nanotubes, Carbon; D009561:Nisin