title: Quantification of Propionibacterium acidipropionici P169 bacteria in environmental samples by use of strain-specific primers derived by suppressive subtractive hybridization.
abstract: A quantitative PCR (qPCR) assay targeting a gene identified by suppressive subtractive hybridization (SSH) was developed to detect Propionibacterium acidipropionici P169, with a threshold of 10(4) CFU/U of dairy feed or rumen fluid. The report is the first using a molecular marker generated by SSH to quantify a bacterial strain in environmental samples.
authors: Danisco Cultures Division, W227 N752 Westmound Drive, Waukesha, WI 53186, USA. min.peng@danisco.com
authors: Min
authors: M
authors: Peng
authors: Alexandra H
authors: AH
authors: Smith
authors: Thomas G
authors: TG
authors: Rehberger
mesh_terms: D058491:Bacterial Load / Q000379:methods*; D017931:DNA Primers / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics*; D004783:Environmental Microbiology; D009693:Nucleic Acid Hybridization / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods*; D011424:Propionibacterium / Q000235:genetics / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial