title: Commercial spray-dried porcine plasma does not transmit porcine circovirus type 2 in weaned pigs challenged with porcine reproductive and respiratory syndrome virus.
abstract: The objective of this study was to evaluate if spray dried porcine plasma (SDPP) containing porcine circovirus type 2 (PCV2) genome supplemented in feed could transmit PCV2 to pigs challenged with porcine reproductive and respiratory syndrome virus (PRRSV). Twenty-three PRRSV-free pigs, non-viraemic for PCV2, were housed in bio-safety level 3 facilities and assigned to four groups in a 2×2 factorial design consisting of PRRSV challenge and a negative control. The diet contained 0 or 8kg SDPP per 100kg of feed. PRRSV challenge groups were inoculated intranasally with 2mL of a suspension containing 10(6) TCID(50)/mL PRRSV. The SDPP used in the study contained 7.56×10(5) PCV2 genome copies per gram. Dietary treatments were fed from 4days prior to PRRSV inoculation until 28days post-inoculation (PI). All challenged pigs developed PRRSV viraemia by day 3PI and PRRSV antibodies were detected in sera by day 14PI, with no difference between diet treatments. Neither PRRSV viraemia nor seroconversion was observed in non-challenged pigs. PCV2 was not detected in the serum of any pigs throughout the experimental period. SDPP containing the PCV2 genome supplemented in feed did not result in PCV2 transmission to either healthy or PRRSV-infected pigs under these experimental conditions.
authors: Institut de Recerca i Tecnologia Agroalimentàries (IRTA), Barcelona, Spain; Centre de Recerca en Sanitat Animal (CReSA), Fundación UAB-IRTA, Campus de la Universitat Autònoma de Barcelona, 08193 Barcelona, Spain.
authors: Joan
authors: J
authors: Pujols
authors: Centre de Recerca en Sanitat Animal (CReSA), Fundación UAB-IRTA, Campus de la Universitat Autònoma de Barcelona, 08193 Barcelona, Spain.
authors: Cristina
authors: C
authors: Lorca-Oró
authors: Centre de Recerca en Sanitat Animal (CReSA), Fundación UAB-IRTA, Campus de la Universitat Autònoma de Barcelona, 08193 Barcelona, Spain.
authors: Ivan
authors: I
authors: Díaz
authors: APC Inc., Ankeny, IA 50021, USA.
authors: Louis E
authors: LE
authors: Russell
authors: APC Inc., Ankeny, IA 50021, USA.
authors: Joy M
authors: JM
authors: Campbell
authors: APC Inc., Ankeny, IA 50021, USA.
authors: Joe D
authors: JD
authors: Crenshaw
authors: APC Europe, S.A., 08403 Granollers, Spain. Electronic address: javier.polo@ampc-europe.com.
authors: Javier
authors: J
authors: Polo
authors: Centre de Recerca en Sanitat Animal (CReSA), Fundación UAB-IRTA, Campus de la Universitat Autònoma de Barcelona, 08193 Barcelona, Spain; Departament de Sanitat i Anatomia Animals, Facultat de Veterinària, Universitat Autònoma de Barcelona (UAB), 08193 Barcelona, Spain.
authors: Enric
authors: E
authors: Mateu
authors: Centre de Recerca en Sanitat Animal (CReSA), Fundación UAB-IRTA, Campus de la Universitat Autònoma de Barcelona, 08193 Barcelona, Spain; Departament de Sanitat i Anatomia Animals, Facultat de Veterinària, Universitat Autònoma de Barcelona (UAB), 08193 Barcelona, Spain.
authors: Joaquim
authors: J
authors: Segalés
mesh_terms: D000704:Analysis of Variance; D000821:Animal Feed / Q000821:virology*; D000818:Animals; D017925:Circovirus / Q000235:genetics; D016679:Genome, Viral; D016867:Immunocompromised Host; D016014:Linear Models; D010949:Plasma / Q000821:virology*; D053570:Porcine Postweaning Multisystemic Wasting Syndrome / Q000276:immunology / Q000635:transmission*; D019318:Porcine Reproductive and Respiratory Syndrome / Q000276:immunology*; D013552:Swine