title: The base excision repair pathway is required for efficient lentivirus integration.
abstract: An siRNA screen has identified several proteins throughout the base excision repair (BER) pathway of oxidative DNA damage as important for efficient HIV infection. The proteins identified included early repair factors such as the base damage recognition glycosylases OGG1 and MYH and the late repair factor POLß, implicating the entire BER pathway. Murine cells with deletions of the genes Ogg1, Myh, Neil1 and Polß recapitulate the defect of HIV infection in the absence of BER. Defective infection in the absence of BER proteins was also seen with the lentivirus FIV, but not the gammaretrovirus MMLV. BER proteins do not affect HIV infection through its accessory genes nor the central polypurine tract. HIV reverse transcription and nuclear entry appear unaffected by the absence of BER proteins. However, HIV integration to the host chromosome is reduced in the absence of BER proteins. Pre-integration complexes from BER deficient cell lines show reduced integration activity in vitro. Integration activity is restored by addition of recombinant BER protein POLß. Lentiviral infection and integration efficiency appears to depend on the presence of BER proteins.
authors: Molecular Virology, Immunology, and Medical Genetics, Human Cancer Genetics, The Ohio State University Medical Center, The Ohio State University, Columbus, Ohio, United States of America. yoder.176@osu.edu
authors: Kristine E
authors: KE
authors: Yoder
authors: Amy
authors: A
authors: Espeseth
authors: Xiao-hong
authors: XH
authors: Wang
authors: Qingming
authors: Q
authors: Fang
authors: Maria Teresa
authors: MT
authors: Russo
authors: R Stephen
authors: RS
authors: Lloyd
authors: Daria
authors: D
authors: Hazuda
authors: Robert W
authors: RW
authors: Sobol
authors: Richard
authors: R
authors: Fishel
mesh_terms: D021581:Active Transport, Cell Nucleus; D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D002470:Cell Survival; D004249:DNA Damage; D004260:DNA Repair / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D017353:Gene Deletion; D015658:HIV Infections / Q000235:genetics; D006801:Humans; D016086:Lentivirus / Q000502:physiology*; D016180:Lentivirus Infections / Q000235:genetics; D051379:Mice; D048348:Reverse Transcription / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics*; D013997:Time Factors; D016662:Virus Integration / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D004279:DNA, Viral