title: Application of factorial designs to study factors involved in the determination of aldehydes present in beer by on-fiber derivatization in combination with gas chromatography and mass spectrometry.
abstract: With the aim of studying the factors involved in on-fiber derivatization of Strecker aldehydes, furfural, and (E)-2-nonenal with O-(2,3,4,5,6-pentafluorobenzyl)hydroxylamine in beer, factorial designs were applied. The effect of the temperature, time, and NaCl addition on the analytes' derivatization/extraction efficiency was studied through a factorial 2(3) randomized-block design; all of the factors and their interactions were significant at the 95% confidence level for most of the analytes. The effect of temperature and its interactions separated the analytes in two groups. However, a single sampling condition was selected that optimized response for most aldehydes. The resulting method, combining on-fiber derivatization with gas chromatography-mass spectrometry, was validated. Limits of detections were between 0.015 and 1.60 μg/L, and relative standard deviations were between 1.1 and 12.2%. The efficacy of the internal standardization method was confirmed by recovery percentage (73-117%). The method was applied to the determination of aldehydes in fresh beer and after storage at 28 °C.
authors: Departamento de Química, Universidad Central de Venezuela, Caracas, Venezuela.
authors: Génesis
authors: G
authors: Carrillo
authors: Adriana
authors: A
authors: Bravo
authors: Carsten
authors: C
authors: Zufall
mesh_terms: D000447:Aldehydes / Q000032:analysis*; D001515:Beer / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D013696:Temperature
chemical_list: D000447:Aldehydes