title: Chemosensory Ca2+ dynamics correlate with diverse behavioral phenotypes in human sperm.
abstract: In the female reproductive tract, mammalian sperm undergo a regulated sequence of prefusion changes that "prime" sperm for fertilization. Among the least understood of these complex processes are the molecular mechanisms that underlie sperm guidance by environmental chemical cues. A "hard-wired" Ca(2+) signaling strategy that orchestrates specific motility patterns according to given functional requirements is an emerging concept for regulation of sperm swimming behavior. The molecular players involved, the spatiotemporal characteristics of such motility-associated Ca(2+) dynamics, and the relation between a distinct Ca(2+) signaling pattern and a behavioral sperm phenotype, however, remain largely unclear. Here, we report the functional characterization of two human sperm chemoreceptors. Using complementary molecular, physiological, and behavioral approaches, we comparatively describe sperm Ca(2+) responses to specific agonists of these novel receptors and bourgeonal, a known sperm chemoattractant. We further show that individual receptor activation induces specific Ca(2+) signaling patterns with unique spatiotemporal dynamics. These distinct Ca(2+) dynamics are correlated to a set of stimulus-specific stereotyped behavioral responses that could play vital roles during various stages of prefusion sperm-egg chemical communication.
authors: Department of Chemosensation, Institute for Biology II, Rheinisch-Westfaelische Technische Hochschule-Aachen University, 52074 Aachen, Germany.
authors: Thomas
authors: T
authors: Veitinger
authors: Jeffrey R
authors: JR
authors: Riffell
authors: Sophie
authors: S
authors: Veitinger
authors: Jaclyn M
authors: JM
authors: Nascimento
authors: Annika
authors: A
authors: Triller
authors: Charlie
authors: C
authors: Chandsawangbhuwana
authors: Katlen
authors: K
authors: Schwane
authors: Andreas
authors: A
authors: Geerts
authors: Frank
authors: F
authors: Wunder
authors: Michael W
authors: MW
authors: Berns
authors: Eva M
authors: EM
authors: Neuhaus
authors: Richard K
authors: RK
authors: Zimmer
authors: Marc
authors: M
authors: Spehr
authors: Hanns
authors: H
authors: Hatt
mesh_terms: D020101:Acrosome Reaction; D001681:Biological Assay; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D002633:Chemotaxis; D005407:Flagella / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D009711:Nucleotides / Q000737:chemistry; D010641:Phenotype; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D013094:Spermatozoa / Q000378:metabolism* / Q000502:physiology; D013737:Testis / Q000378:metabolism
chemical_list: D009711:Nucleotides; D002118:Calcium