title: [Determination of magnoflorine in Coptidis Rhizoma and Phellodendri Chinensis Cortex by LC-MS].
abstract: A rapid and specific high performance liquid chromatography-mass spectrometric method was developed for determination of magnoflorine in Rhizoma Coptidis and Cortex Phellodendri Chinensis. Samples were extracted by methanol. Agilent Eclipse XDB-C18 ODS column (4.6 mm x 150 mm, 5 microm) was used, and mobile phase was methanol-water (60:40) at a flow rate of 0.8 mL x min(-1). Electrospray ionization model (ESI), and MRM model were used for quantification. The linear range of magnoflorine was 4.352-2720 microg x L(-1). The average recovery was above 98%. The method is simple, sensitive and accurate, it can be used for determination of magnoflorine in Rhizoma Coptidis and Cortex Phellodendri Chinensis.
authors: Department of Pharmacology, Harbin Medical University, Harbin 150086, China.
authors: Shengjun
authors: S
authors: Fan
authors: Dahai
authors: D
authors: Yu
authors: Yuanqinl
authors: Y
authors: Gu
authors: Mingyu
authors: M
authors: Zhang
authors: Lin
authors: L
authors: Zhang
authors: Guoyu
authors: G
authors: Li
mesh_terms: D001060:Aporphines / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D000089042:Coptis chinensis; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D016014:Linear Models; D013058:Mass Spectrometry / Q000379:methods*; D013997:Time Factors
chemical_list: D001060:Aporphines; D004365:Drugs, Chinese Herbal; C528424:huangbai; C107393:Coptidis rhizoma extract; C001670:magnoflorine