title: Exopolysaccharide production by a genetically engineered Enterobacter cloacae strain for microbial enhanced oil recovery.
abstract: Microbial enhanced oil recovery (MEOR) is a petroleum biotechnology for manipulating function and/or structure of microbial environments existing in oil reservoirs for prolonged exploitation of the largest source of energy. In this study, an Enterobacter cloacae which is capable of producing water-insoluble biopolymers at 37°C and a thermophilic Geobacillus strain were used to construct an engineered strain for exopolysaccharide production at higher temperature. The resultant transformants, GW3-3.0, could produce exopolysaccharide up to 8.83 g l(-1) in molasses medium at 54°C. This elevated temperature was within the same temperature range as that for many oil reservoirs. The transformants had stable genetic phenotype which was genetically fingerprinted by RAPD analysis. Core flooding experiments were carried out to ensure effective controlled profile for the simulation of oil recovery. The results have demonstrated that this approach has a promising application potential in MEOR.
authors: State Key Laboratory of Heavy Oil Processing, Faculty of Chemical Engineering, China University of Petroleum, Beijing 102249, PR China.
authors: Shanshan
authors: S
authors: Sun
authors: Zhongzhi
authors: Z
authors: Zhang
authors: Yijing
authors: Y
authors: Luo
authors: Weizhang
authors: W
authors: Zhong
authors: Meng
authors: M
authors: Xiao
authors: Wenjing
authors: W
authors: Yi
authors: Li
authors: L
authors: Yu
authors: Pengcheng
authors: P
authors: Fu
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D016972:Enterobacter cloacae / Q000235:genetics / Q000378:metabolism*; D006358:Hot Temperature; D030781:Organisms, Genetically Modified; D016133:Polymerase Chain Reaction; D011134:Polysaccharides / Q000096:biosynthesis*
chemical_list: D017931:DNA Primers; D011134:Polysaccharides