title: A conserved patch near the C terminus of histone H4 is required for genome stability in budding yeast.
abstract: A screen of Saccharomyces cerevisiae histone alanine substitution mutants revealed that mutations in any of three adjacent residues, L97, Y98, or G99, near the C terminus of H4 led to a unique phenotype. The mutants grew slowly, became polyploid or aneuploid rapidly, and also lost chromosomes at a high rate, most likely because their kinetochores were not assembled properly. There was lower histone occupancy, not only in the centromeric region, but also throughout the genome for the H4 mutants. The mutants displayed genetic interactions with the genes encoding two different histone chaperones, Rtt106 and CAF-I. Affinity purification of Rtt106 and CAF-I from yeast showed that much more H4 and H3 were bound to these histone chaperones in the case of the H4 mutants than in the wild type. However, in vitro binding experiments showed that the H4 mutant proteins bound somewhat more weakly to Rtt106 than did wild-type H4. These data suggest that the H4 mutant proteins, along with H3, accumulate on Rtt106 and CAF-I in vivo because they cannot be deposited efficiently on DNA or passed on to the next step in the histone deposition pathway, and this contributes to the observed genome instability and growth defects.
authors: Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY 11794, USA.
authors: Yao
authors: Y
authors: Yu
authors: Madhusudhan
authors: M
authors: Srinivasan
authors: Shima
authors: S
authors: Nakanishi
authors: Janet
authors: J
authors: Leatherwood
authors: Ali
authors: A
authors: Shilatifard
authors: Rolf
authors: R
authors: Sternglanz
mesh_terms: D020816:Amino Acid Motifs; D019943:Amino Acid Substitution; D002843:Chromatin; D005434:Flow Cytometry; D016681:Genome, Fungal; D042822:Genomic Instability; D006657:Histones / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D018386:Kinetochores / Q000648:ultrastructure; D018832:Molecular Chaperones / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D010641:Phenotype; D011003:Ploidies; D011485:Protein Binding; D012260:Ribonucleases / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*
chemical_list: D002843:Chromatin; D006657:Histones; D018832:Molecular Chaperones; C505157:Rtt106 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D012260:Ribonucleases; C079009:POP2 protein, S cerevisiae