title: Magnetic mesoporous organic-inorganic NiCo2O4 hybrid nanomaterials for electrochemical immunosensors.
abstract: This study demonstrates a facile and feasible strategy toward the development of advanced electrochemical immunosensors based on chemically functionalized magnetic mesoporous organic-inorganic hybrid nanomaterials, and the preparation, characterization, and measurement of relevant properties of the immunosensor for detection of carcinoembryonic antigen (CEA, as a model analyte) in clinical immunoassays. The as-prepared nanomaterials composed of a magnetic mesoporous NiCo(2)O(4) nanosheet, an interlayer of Nafion/thionine organic molecules and a nanogold layer show good adsorption properties for the attachment of horseradish peroxidase-labeled secondary anti-CEA antibody (HRP-anti-CEA). With a sandwich-type immunoassay format, the functional bionanomaterials present good analytical properties to facilitate and modulate the way it was integrated onto the electrochemical immunosensors, and allows the detection of CEA at a concentration as low as 0.5 pg/mL. Significantly, the immunosensor could be easily regenerated by only using an external magnet without the need of any dissociated reagents. Importantly, the as-synthesized magnetic mesoporous NiCo(2)O(4) nanomaterials could be further extended for detection of other biomarkers or biocompounds.
authors: Key Laboratory of Analysis and Detection for Food Safety (Ministry of Education & Fujian Province), Department of Chemistry, Fuzhou University, Fuzhou 350108, China.
authors: Qunfang
authors: Q
authors: Li
authors: Lingxing
authors: L
authors: Zeng
authors: Jinchao
authors: J
authors: Wang
authors: Dianping
authors: D
authors: Tang
authors: Bingqian
authors: B
authors: Liu
authors: Guonan
authors: G
authors: Chen
authors: Mingdeng
authors: M
authors: Wei
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002272:Carcinoembryonic Antigen / Q000032:analysis; D019411:Clinical Laboratory Techniques / Q000379:methods*; D055664:Electrochemical Techniques / Q000379:methods*; D006735:Horseradish Peroxidase / Q000378:metabolism; D007118:Immunoassay / Q000379:methods; D008280:Magnetics; D053761:Nanocomposites / Q000737:chemistry*; D058608:Nanopores; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000379:methods
chemical_list: D002272:Carcinoembryonic Antigen; D006735:Horseradish Peroxidase