title: [Genetic diversity of the genus Lactobacillus bacteria from the human gastrointestinal microbiome].
abstract: The species and strain genetic diversity of bacterial cultures belonging to the genus Lactobacillus, which were isolated from the gastrointestinal microbiome of the human population living in the former Soviet Union in the years 1960-1980, was studied. The bacteria demonstrated probiotic characteristics. Phylogenetic analysis of sequences of the gene coding for 16S rRNA detected earlier by us, showed that the gene found in bacteria isolated from the intestinal content of healthy adults and represented by species L. plantarum, L. helveticus, L. casei/paracasei, L. rhamnosus, and L. fermentum has high homology (97-100%) with this gene in type representatives of the species. The genotypic and strain diversity of cultures was studied using RAPD-PCR and nonspecific primers. This method with the use of the ERIC-1 primer gave reliable and reproducible results as compared that using with M13 and MSP primers and allowed the identification of examined bacteria belonging to the genus Lactobacillus at the level of species and certification at the strain level.
authors: S G
authors: SG
authors: Botina
authors: N V
authors: NV
authors: Koroban
authors: K M
authors: KM
authors: Klimina
authors: A A
authors: AA
authors: Glazova
authors: N V
authors: NV
authors: Zakharevich
authors: V V
authors: VV
authors: Zinchenko
authors: V N
authors: VN
authors: Danilenko
mesh_terms: D001483:Base Sequence; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D007778:Lactobacillus / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S