title: Evaluation of the biocompatibility of S-phase layers on medical grade austenitic stainless steels.
abstract: S-phase surface layers were formed in AISI 316LVM (ASTM F138) and High-N (ASTM F1586) medical grade austenitic stainless steels by plasma surface alloying with nitrogen (at 430°C), carbon (at 500°C) and both carbon and nitrogen (at 430°C). The presence of the S-phase was confirmed by microscopy, hardness testing, depth-profile analysis of chemical composition and X-ray Diffraction. Attachment and proliferation of mouse osteoblast MC3T3-E1 cells were tested on S-phase and untreated controls and the results demonstrated that all the S-phase layers formed were biocompatible under the conditions used. Cells adhered equally well to all samples but proliferation was enhanced on the treated materials.
authors: School of Metallurgy and Materials, The University of Birmingham, Birmingham, B15 2TT, UK. joseph.p.buhagiar@um.edu.mt
authors: Joseph
authors: J
authors: Buhagiar
authors: Thomas
authors: T
authors: Bell
authors: Rachel
authors: R
authors: Sammons
authors: Hanshan
authors: H
authors: Dong
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001672:Biocompatible Materials; D002448:Cell Adhesion; D049109:Cell Proliferation; D051379:Mice; D008855:Microscopy, Electron, Scanning; D013193:Stainless Steel; D013499:Surface Properties; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D013193:Stainless Steel