title: Antibacterial activity of metabolite produced by Paenibacillus polymyxa strain HKA-15 against Xanthomonas campestris pv. phaseoli.
abstract: An antibacterial metabolite extracted from Paenibacillus polymyxa HKA-15 showed strong inhibition against Xanthomonas campestris pv. phaseoli strains CP-1-1 and M-5. Minimum inhibitory concentration (MIC) of crude extract against strains CP-1-1 and M-5 was found to be 1.7 mg/ml and 1.52 mg/ml, respectively. In UV-Vis range, the absorption peak of crude extract was maximum at 240 nm. The compound is resilience to wide range of temperature, pH, surfactants and organic solvents. The complete loss of activity was observed when crude metabolite was treated with pepsin (400 unit/ml). Characterization of crude metabolite suggested its hydrophobic and peptide nature. Inhibition of Xanthomonas campestris pv. phaseoli by peptide like metabolite produced by Paenibacillus polymyxa strain HKA-15 under in vitro conditions showed ecological and biotechnological potential of strain HKA-15 to control common blight disease in beans.
authors: Chemical and Biochemical Processing Division, CIRCOT, Adenwala Road, Matunga, Mumbai 400 019, India. mageshbio@yahoo.co.in
authors: V
authors: V
authors: Mageshwaran
authors: Suresh
authors: S
authors: Walia
authors: V
authors: V
authors: Govindasamy
authors: K
authors: K
authors: Annapurna
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D056507:Paenibacillus / Q000378:metabolism*; D010935:Plant Diseases / Q000382:microbiology / Q000517:prevention & control; D013025:Glycine max / Q000382:microbiology; D016959:Xanthomonas campestris / Q000187:drug effects* / Q000472:pathogenicity
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins