title: Amine-reactive PEGylated nanoparticles for potential bioconjugation.
abstract: Water-dispersible PEGylated nanoparticles (NPs) presenting amine-reactive conjugation sites at their surfaces were synthesized and their ability to react with amines was demonstrated. An amphiphilic block copolymer bearing an N-succinimidyl ester at its water-soluble end was synthesized by the consecutive controlled radical polymerization of poly(ethylene glycol) methacrylate and styrene from a functional halide initiator. After purification of the copolymer, NPs of approximately 40 nm were obtained by a self-assembly process in water. The reactivity of the NPs was evidenced by reacting them with primary amines, including a fluorescent dye. The activated ester remained stable throughout all synthetic steps and a nearly quantitative coupling efficiency was obtained.
authors: Radboud University Nijmegen, Institute for Molecules and Materials, Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands. guillaume.delaittre@kit.edu
authors: Guillaume
authors: G
authors: Delaittre
authors: Gemma
authors: G
authors: Justribó-Hernández
authors: Roeland J M
authors: RJ
authors: Nolte
authors: Jeroen J L M
authors: JJ
authors: Cornelissen
mesh_terms: D000588:Amines / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D011092:Polyethylene Glycols / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D014867:Water / Q000737:chemistry
chemical_list: D000588:Amines; D005456:Fluorescent Dyes; C450003:poly(ethylene glycol) methacrylate polymer; D014867:Water; D011092:Polyethylene Glycols