title: The staining pattern of human sperm with Diff Quik: relationship with sperm head morphology and a sperm chromatin structure assay (SCSA).
abstract: The dark staining of human sperm heads by Diff Quik is significantly correlated with abnormal sperm head morphology (r=0.51, p<0.0001), but is not associated with changes in sperm chromatin detected by a sperm chromatin structure assay (SCSA; r=0.18, p>0.09). Whilst valuable in the assessment of head morphology, it is concluded that Diff Quik staining is not a useful substitute for the SCSA to assess human sperm chromatin.
authors: Hollywood Fertility Centre, Hollywood Private Hospital, Nedlands 6009, Western Australia, Australia. natalie.moska@hollywoodivf.com
authors: Natalie
authors: N
authors: Moska
authors: Elaine
authors: E
authors: Murray
authors: Penelope
authors: P
authors: Wakefield
authors: Phillip
authors: P
authors: Matson
mesh_terms: D001399:Azure Stains; D002843:Chromatin / Q000502:physiology*; D006801:Humans; D008297:Male; D008751:Methylene Blue; D013077:Sperm Head / Q000502:physiology*; D013094:Spermatozoa / Q000166:cytology*; D013194:Staining and Labeling; D014966:Xanthenes
chemical_list: D001399:Azure Stains; D002843:Chromatin; C067461:Diff Quik; D014966:Xanthenes; D008751:Methylene Blue