title: Quantitation of resveratrol in red wines by means of stable isotope dilution analysis-ultra-performance liquid chromatography-Quan-time-of-flight mass spectrometry and cross validation.
abstract: A stable isotope dilution analysis (SIDA) was developed for the quantitative analysis of the health-promoting phytoalexin (E)-resveratrol in red wines by means of UPLC-QuanTOF-MS. After hemisynthetic preparation of (E)-3,5,4'-trihydroxy-2,4,6-trideuterostilbene ((E)-[(2)H(3)]-resveratrol) as the stable isotope labeled internal standard, validation experiments revealed recovery rate of 96.2 ± 0.8% RSD, thus demonstrating the robustness and accuracy of the SIDA-UPLC-QuanTOF-MS method. Repeatability and reproducibility expressed as RSD showed excellent values of 3.0% and 4.0% for (E)-[(2)H(3)]-resveratrol. Cross validation against a SIDA-HPLC-MS/MS analysis using a triple quadrupole mass spectrometer revealed comparable data, but the SIDA-UPLC-QuanTOF-MS was four times faster, thus making the latter method preferential for an accurate high-throughput analysis of wine samples. Comparison of the SIDA data to those obtained by quantitation using a standard addition method and external calibration, respectively, revealed 97.7% and 32.4% of the resveratrol concentration determined by means of SIDA-UPLC-QuanTOF-MS and 101.0% and 12.7% of the resveratrol levels found by using SIDA-HPLC-MS/MS.
authors: Chair of Food Chemistry and Molecular Sensory Science, Technische Universität München, Freising, Germany. timo.stark@tum.de
authors: Timo
authors: T
authors: Stark
authors: Nadine
authors: N
authors: Wollmann
authors: Sofie
authors: S
authors: Lösch
authors: Thomas
authors: T
authors: Hofmann
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards; D003903:Deuterium; D007201:Indicator Dilution Techniques; D013058:Mass Spectrometry / Q000379:methods* / Q000592:standards; D012015:Reference Standards; D015203:Reproducibility of Results; D000077185:Resveratrol; D013237:Stereoisomerism; D013267:Stilbenes / Q000032:analysis* / Q000737:chemistry; D013997:Time Factors; D014920:Wine / Q000032:analysis*
chemical_list: D013267:Stilbenes; D003903:Deuterium; D000077185:Resveratrol