title: Schwann cells promote neurite outgrowth of dorsal root ganglion neurons through secretion of nerve growth factor.
abstract: The transplantation of Schwann cells (SCs) could successfully promote axonal regeneration. This is likely to attribute to the adhesion molecules expression and growth factors secretion of SCs. But which factor(s) play a key role has not been precisely studied. In this study, an outgrowth assay using dorsal root ganglia (DRG) neuron-SC co-culture system in vitro was performed. Co-culture of SCs or application of SC-conditioned medium (CM) substantially and significantly increased DRG neurite outgrowth. Further, nerve growth factor and NGF receptor (TrkA) mRNA were highly expressed in Schwann cells and DRG neuron, respectively. The high concentration of NGF protein was detected in SC-CM. When K-252a, a specific inhibitor of NGF receptor was added, DRG neurite outgrowth was significantly decreased in a concentration-dependent manner. These data strongly suggest that SCs play important roles in neurite outgrowth of DRG neurons by secreted NGF.
authors: Department of Clinical Laboratory Science, The First Affiliated Hospital of Bengbu Medical College, 287 Changhuai Road Bengbu 233004, PR China. jghu.hzlv@yahoo.com
authors: Jianguo
authors: J
authors: Hu
authors: Jiansheng
authors: J
authors: Zhou
authors: Xingwu
authors: X
authors: Li
authors: Fengchao
authors: F
authors: Wang
authors: Hezuo
authors: H
authors: Lü
mesh_terms: D000818:Animals; D002227:Carbazoles / Q000494:pharmacology; D018920:Coculture Techniques; D017077:Culture Media, Conditioned; D005727:Ganglia, Spinal / Q000187:drug effects / Q000502:physiology*; D026121:Indole Alkaloids / Q000494:pharmacology; D020932:Nerve Growth Factor / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D009416:Nerve Regeneration / Q000187:drug effects / Q000502:physiology; D016501:Neurites / Q000187:drug effects / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D020917:Receptor, trkA / Q000037:antagonists & inhibitors / Q000235:genetics; D012583:Schwann Cells / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology
chemical_list: D002227:Carbazoles; D017077:Culture Media, Conditioned; D026121:Indole Alkaloids; D012333:RNA, Messenger; D020932:Nerve Growth Factor; C049985:staurosporine aglycone; D020917:Receptor, trkA