title: Interfacing cell-based assays in environmental scanning electron microscopy using dielectrophoresis.
abstract: Development of the dielectrophoretic (DEP) live cell trapping technology and its interfacing with the environmental scanning electron microscopy (ESEM) is described. DEP microelectrode arrays were fabricated on glass substrate using photolithography and lift-off. Chip-based arrays were applied for ESEM analysis of DEP-trapped human leukemic cells. This work provides proof-of-concept interfacing of the DEP cell retention and trapping technology with ESEM to provide a high-resolution analysis of individual nonadherent cells.
authors: Department of Chemistry and MacDiarmid Institute for Advanced Materials and Nanotechnology, University of Auckland, Auckland, New Zealand. khashayar.khoshmanesh@deakin.edu.au
authors: Khashayar
authors: K
authors: Khoshmanesh
authors: Jin
authors: J
authors: Akagi
authors: Saeid
authors: S
authors: Nahavandi
authors: Kourosh
authors: K
authors: Kalantar-zadeh
authors: Sara
authors: S
authors: Baratchi
authors: David E
authors: DE
authors: Williams
authors: Jonathan M
authors: JM
authors: Cooper
authors: Donald
authors: D
authors: Wlodkowic
mesh_terms: D004586:Electrophoresis; D006801:Humans; D007938:Leukemia / Q000473:pathology*; D008839:Microelectrodes; D008855:Microscopy, Electron, Scanning / Q000295:instrumentation; D013499:Surface Properties; D014407:Tumor Cells, Cultured; D020298:U937 Cells