title: Detection of respiratory viruses by PCR assay of nasopharyngeal swabs stored in skim milk-tryptone-glucose-glycerol transport medium.
abstract: We analyzed 129 paired nasopharyngeal aspirates (stored in viral transport medium [VTM]) and nasopharyngeal swabs (stored in skim milk-tryptone-glucose-glycerol [STGG] bacterial transport and storage medium) using PCRs to detect adenoviruses, influenza virus A or B, and respiratory syncytial virus (RSV). Overall, swabs stored in STGG medium without antimicrobials were found to be an acceptable alternative to aspirates stored in antimicrobial-containing VTM, with PCR agreement of 90.2% (kappa of 0.8).
authors: Shoko Malaria Research Unit, Mae Sot, Thailand. pault@tropmedres.ac
authors: Paul
authors: P
authors: Turner
authors: Linda
authors: L
authors: Po
authors: Claudia
authors: C
authors: Turner
authors: David
authors: D
authors: Goldblatt
authors: Francois
authors: F
authors: Nosten
mesh_terms: D002675:Child, Preschool; D006801:Humans; D007223:Infant; D009305:Nasopharynx / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods; D012141:Respiratory Tract Infections / Q000821:virology*; D013048:Specimen Handling / Q000379:methods*; D014773:Virology / Q000379:methods*; D014777:Virus Diseases / Q000175:diagnosis* / Q000821:virology*; D014780:Viruses / Q000302:isolation & purification*