title: Detection of human papillomavirus E6/E7 mRNA in women with high-risk HPV types 16, 18, 31, 33 and 45 which are associated with the development of human cervical cancer.
abstract: OBJECTIVE
  The aim of our study was to increase the clinical meaningfulness of the virological data through mRNA E6/E7 oncoprotein identification, and to find a correlation between codon 72 polymorphism of the p53 gene and integration of HPV in host cell genomes.
  
  
  METHODS
  We analyzed 80 cervical samples from women with HPV DNA types 16, 18, 31, 33 and 45. Transcripts of HPV were detected by the NucliSense EasyQ HPV assay and genotyping of the TP53 polymorphism was conducted using a TaqMan assay.
  
  
  RESULTS
  Twenty percent of 80 tested samples were positive for mRNA Papillomavirus. The frequency of Arg/Pro heterozygotes in controls was over-represented compared with mRNA positive samples while there were no significant differences in the distribution of Pro/Pro and Arg/Arg alleles.
  
  
  CONCLUSIONS
  The introduction of HPV mRNA testing in clinical analysis improved diagnostic accuracy of HPV infections. Our data suggest that a structural difference at codon 72 of the p53 gene may not be a sufficient risk factor for cervical carcinogenesis.
authors: Department of Hygiene, Preventive Medicine and Public Health, University of Messina, Messina, Italy. m.bertuccio@email.it
authors: M P
authors: MP
authors: Bertuccio
authors: P
authors: P
authors: Spataro
authors: C
authors: C
authors: Caruso
authors: I
authors: I
authors: Picerno
mesh_terms: D000293:Adolescent; D000328:Adult; D003062:Codon; D004268:DNA-Binding Proteins / Q000235:genetics; D005260:Female; D016158:Genes, p53; D052162:Human papillomavirus 16 / Q000235:genetics; D052161:Human papillomavirus 18 / Q000235:genetics; D006801:Humans; D008875:Middle Aged; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D050725:Papillomavirus E7 Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis*; D012367:RNA, Viral / Q000032:analysis*; D012097:Repressor Proteins / Q000235:genetics; D002578:Uterine Cervical Dysplasia / Q000821:virology*; D002583:Uterine Cervical Neoplasms / Q000821:virology*; D014759:Viral Envelope Proteins / Q000235:genetics
chemical_list: D003062:Codon; D004268:DNA-Binding Proteins; C524477:E6 protein, HPV-33; C068651:E6 protein, Human papillomavirus type 16; C052603:E6 protein, Human papillomavirus type 18; C052633:E7 protein, Human papillomavirus type 18; C502189:E7 protein, Human papillomavirus type 31; C410917:E7 protein, Human papillomavirus type 33; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D012333:RNA, Messenger; D012367:RNA, Viral; D012097:Repressor Proteins; D014759:Viral Envelope Proteins; C059731:oncogene protein E7, Human papillomavirus type 16; C514514:oncogene protein E7, human papilloma virus type 45