title: Lutibacter aestuarii sp. nov., isolated from a tidal flat sediment, and emended description of the genus Lutibacter Choi and Cho 2006.
abstract: A gram-negative, non-spore-forming, non-motile rod, designated MA-My1(T), was isolated from a tidal flat sediment of the South Sea, Korea. Strain MA-My1(T) grew optimally at pH 7.0-7.5, at 30 °C and with 2.0-3.0 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain MA-My1(T) clustered with Lutibacter litoralis CL-TF09(T) and Lutibacter maritimus S7-2(T), with which it exhibited 97.3 and 95.3 % 16S rRNA gene sequence similarity, respectively. Strain MA-My1(T) contained MK-6 as the predominant menaquinone and iso-C(15 : 0), iso-C(15 : 0) 3-OH and iso-C(16 : 0) 3-OH as the major fatty acids. The major polar lipids were phosphatidylethanolamine and two unidentified lipids. The DNA G+C content was 30.6 mol% and DNA-DNA relatedness between strain MA-My1(T) and L. litoralis JCM 13034(T) was 6.3±0.8 %. The differential phenotypic properties, together with phylogenetic and genotypic distinctiveness, distinguished strain MA-My1(T) from the members of the genus Lutibacter. On the basis of the data presented, strain MA-My1(T) is considered to represent a novel species of the genus Lutibacter, for which the name Lutibacter aestuarii sp. nov. is proposed. The type strain is MA-My1(T) ( = KCTC 23499(T)  = CCUG 60022(T)).
authors: Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejeon, Republic of Korea.
authors: Soo-Young
authors: SY
authors: Lee
authors: Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejeon, Republic of Korea.
authors: Mi-Hwa
authors: MH
authors: Lee
authors: Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejeon, Republic of Korea.
authors: Tae-Kwang
authors: TK
authors: Oh
authors: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.|Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejeon, Republic of Korea.
authors: Jung-Hoon
authors: JH
authors: Yoon
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D041965:Flavobacteriaceae / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D020459:Genes, rRNA; D019015:Geologic Sediments / Q000382:microbiology*; D008055:Lipids / Q000032:analysis; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D008055:Lipids; D011809:Quinones; D012336:RNA, Ribosomal, 16S