title: Development of urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) measurement method combined with SPE.
abstract: A novel separation method of urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) was developed. The C(18) and the strong cation-exchange (SCX) columns were employed to separate urinary 8-OHdG. The major interfering substances were removed by the consecutive processes of the C(18) and the SCX columns. This newly developed procedure allows researchers to quantitatively measure urinary 8-OHdG by high performance liquid chromatography-electrochemical detector (HPLC-ECD) successfully. The newly developed separation method produces the optimized procedure of 8-OHdG measurement, and followed by the evaluation of its basic performance. The optimized measurement was highly reproducible (CV=2.0-2.9%, n=10). A correlation was observed between the proposed HPLC-ECD method and the column switching method (r=0.96).
authors: National Institute of Advanced Industrial Science and Technology (AIST), c/o Katayanagi Advanced Research Laboratories, Tokyo University of Technology, Hachioji, Tokyo 192-0982, Japan.
authors: Yohei
authors: Y
authors: Inaba
authors: Satoshi
authors: S
authors: Koide
authors: Kenji
authors: K
authors: Yokoyama
authors: Isao
authors: I
authors: Karube
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D002851:Chromatography, High Pressure Liquid; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine; D006801:Humans; D016014:Linear Models; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods*
chemical_list: D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine