title: Detection and characterization of ubiquitylated H2B in mammalian cells.
abstract: Histone H2B ubiquitylation was shown to be associated with actively transcribed genes in mammalian cells and has been suggested to be involved in transcriptional regulation. Despite the limited applicability of genetic tools to analyze H2B ubiquitylation in mammals, several biochemical and immunological approaches have been successfully implemented to study this modification. Here we describe several techniques to detect ubiquitylated H2B in mammalian cells and to dissect its genomic localization.
authors: Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel. efrat.shema@weizmann.ac.il
authors: Efrat
authors: E
authors: Shema
authors: Moshe
authors: M
authors: Oren
authors: Neri
authors: N
authors: Minsky
mesh_terms: D053000:Analytic Sample Preparation Methods; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000737:chemistry; D015153:Blotting, Western; D002457:Cell Extracts / Q000737:chemistry; D002459:Cell Fusion; D002467:Cell Nucleus / Q000737:chemistry; D002478:Cells, Cultured; D002843:Chromatin / Q000737:chemistry / Q000378:metabolism; D047369:Chromatin Immunoprecipitation; D005260:Female; D006657:Histones / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D006825:Hybridomas; D051379:Mice; D008807:Mice, Inbred BALB C; D010446:Peptide Fragments / Q000737:chemistry; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D057149:Ubiquitinated Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D002457:Cell Extracts; D002843:Chromatin; D006657:Histones; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; D057149:Ubiquitinated Proteins