title: Isolation and characterization of human periodontal ligament (PDL) stem cells (PDLSCs) from the inflamed PDL tissue: in vitro and in vivo evaluations.
abstract: OBJECTIVE
  Mesenchymal stem cells (MSC) could be isolated from healthy periodontal ligaments (PDL). The aims of this study were to isolate and characterize human PDL stem cells (hPDLSCs) from inflamed PDL tissue, and to evaluate their regenerative potential.
  
  
  METHODS
  Inflamed hPDLSCs (ihPDLSCs) were isolated from the inflamed PDL tissue obtained from intra-bony defects during flap surgery, and characterized by immunohistochemical staining, colony-forming unit assay, fluorescence-activated cell sorting, and mRNA expression in comparison with healthy hPDLSCs obtained from extracted teeth for orthodontic purpose. The proliferative potential and migratory potential was evaluated, and compared with healthy hPDLSCs. Regenerative potential was assessed by an in vivo ectopic transplantation model.
  
  
  RESULTS
  ihPDLSCs were successfully isolated and characterized as MSCs. Both ihPDLSCs and hPDLSCs were successfully differentiated under osteogenic/cementogenic and adipogenic microenvironment. The proliferative potential did not differ between healthy hPDLSCs and ihPDLSCs, while the migratory capacity was significantly increased in ihPDLSCs (p<0.05). Both groups exhibited new cementum-like tissue and related PDL fibre regeneration in an in vivo transplantation model.
  
  
  CONCLUSIONS
  ihPDLSCs could be successfully isolated from inflamed PDL tissue, and they retained the regenerative potential for cementum and related PDL tissues.
authors: Department of Periodontology, Research Institute for Periodontal Regeneration, College of Dentistry, Yonsei University, Seodaemun-gu Seoul, Korea.
authors: Jung-Chul
authors: JC
authors: Park
authors: Jeong-Min
authors: JM
authors: Kim
authors: Im-Hee
authors: IH
authors: Jung
authors: Jane C
authors: JC
authors: Kim
authors: Seong-Ho
authors: SH
authors: Choi
authors: Kyoo-Sung
authors: KS
authors: Cho
authors: Chang-Sung
authors: CS
authors: Kim
mesh_terms: D050156:Adipogenesis / Q000502:physiology; D000293:Adolescent; D000328:Adult; D016301:Alveolar Bone Loss / Q000473:pathology / Q000601:surgery; D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation; D002469:Cell Separation; D025021:Cementogenesis / Q000502:physiology; D055113:Chronic Periodontitis / Q000473:pathology*; D003114:Colony-Forming Units Assay; D005434:Flow Cytometry; D006097:Granulation Tissue / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D051379:Mice; D008875:Middle Aged; D010012:Osteogenesis / Q000502:physiology; D010513:Periodontal Ligament / Q000166:cytology*; D010514:Periodontal Pocket / Q000473:pathology / Q000601:surgery; D012038:Regeneration / Q000502:physiology; D013524:Surgical Flaps / Q000473:pathology; D055815:Young Adult
chemical_list: D000954:Antigens, Surface