title: Changes in the subgingival biofilm composition after coronally positioned flap.
abstract: OBJECTIVE
  This study evaluated the effects of coronally positioned flap (CPF) on the subgingival biofilm composition.
  
  
  METHODS
  Twenty-two subjects with gingival recessions were treated with CPF. Clinical parameters were assessed before and at 6 months after surgery. Subgingival biofilms were analyzed by checkerboard DNA-DNA hybridization technique for 40 bacterial species.
  
  
  RESULTS
  Recession height, clinical attachment level and bleeding on probing improved significantly (p<0.05) at 6 months post-CPF. The proportions of 10 periodontal pathogens and the proportions of red and orange complexes decreased at 6 months.
  
  
  CONCLUSIONS
  In conclusion, CPF can induce beneficial effects on the composition of the subgingival microbiota after 6 months.
authors: Department of Periodontics, Dental Research Division, Guarulhos University, Guarulhos, SP, Brazil.
authors: Jadson Almeida
authors: JA
authors: Lima
authors: Vanessa Renata
authors: VR
authors: Santos
authors: Magda
authors: M
authors: Feres
authors: Luciene Cristina
authors: LC
authors: de Figueiredo
authors: Poliana Mendes
authors: PM
authors: Duarte
mesh_terms: D000328:Adult; D058491:Bacterial Load; D018441:Biofilms / Q000254:growth & development*; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics; D005260:Female; D005881:Gingiva / Q000382:microbiology* / Q000601:surgery; D005889:Gingival Recession / Q000382:microbiology* / Q000601:surgery; D006801:Humans; D008297:Male; D054892:Metagenome; D008875:Middle Aged; D018709:Statistics, Nonparametric; D013524:Surgical Flaps / Q000382:microbiology*; D013997:Time Factors
chemical_list: D015342:DNA Probes; D004269:DNA, Bacterial