title: Short-term antimicrobial properties of mineral trioxide aggregate with incorporated silver-zeolite.
abstract: The purpose of this in vitro study was to determine whether adding silver-zeolite (SZ) to mineral trioxide aggregate (MTA) would enhance the antimicrobial activity of MTA against Staphylococcus aureus (ATCC #25923), Enterococcus faecalis (ATCC #29212), Escherichia coli (ATCC#25922), Pseudomonas aeruginosa (ATCC #27853), Candida albicans (ATCC #90028), Porphyromonas gingivalis (ATCC #33277), Actinomyces israelii (ATCC #12102), and Prevotella intermedia (ATCC# 15032). SZ was added at 0.2% and 2% mass fraction concentration to MTA powder. The control group was MTA powder with no SZ. The antimicrobial effect test was accomplished by placing freshly mixed MTA specimens on agar plates inoculated with microorganisms and comparing the zones of inhibition at 24, 48, and 72 h. The amounts of silver ion release from MTA specimens were measured with atomic absorption spectrophotometry at 10-min, 24-, 48-, and 72-h periods. The pH of MTA specimens was measured with a pH meter at 10-min, 24-, 48-, and 72-h periods. MTA with 2% and 0.2% SZ specimens showed inhibitory effects on some microorganisms at all time periods, whereas no antimicrobial activity showed for P. intermedia and A. israelii. MTA without SZ inhibited C. albicans, E. Coli, and P. intermedia. The highest silver release was detected in 2% SZ MTA at 24 h. The incorporation of SZ may enhance the antimicrobial activity of MTA.
authors: Departments of Pediatric Dentistry Microbiology, Faculty of Dentistry, University of Gazi Department of Chemical Engineering, Faculty of Engineering, University of Middle East Technical, Ankara, Turkey. mesut@gazi.edu.tr
authors: Mesut E
authors: ME
authors: Odabaş
authors: Cağdaş
authors: C
authors: Cinar
authors: Gülçin
authors: G
authors: Akça
authors: Ibrahim
authors: I
authors: Araz
authors: Tezer
authors: T
authors: Ulusu
authors: Hayrettin
authors: H
authors: Yücel
mesh_terms: D000190:Actinomyces / Q000187:drug effects; D017607:Aluminum Compounds / Q000737:chemistry / Q000494:pharmacology*; D000890:Anti-Infective Agents / Q000494:pharmacology*; D017610:Calcium Compounds / Q000737:chemistry / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects; D003470:Culture Media; D004058:Diffusion; D004338:Drug Combinations; D013293:Enterococcus faecalis / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008422:Materials Testing; D010087:Oxides / Q000737:chemistry / Q000494:pharmacology*; D016966:Porphyromonas gingivalis / Q000187:drug effects; D018713:Prevotella intermedia / Q000187:drug effects; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D012387:Root Canal Filling Materials / Q000737:chemistry / Q000494:pharmacology*; D017640:Silicates / Q000737:chemistry / Q000494:pharmacology*; D012834:Silver / Q000737:chemistry / Q000494:pharmacology*; D013054:Spectrophotometry, Atomic; D013211:Staphylococcus aureus / Q000187:drug effects; D013997:Time Factors; D017641:Zeolites / Q000494:pharmacology*
chemical_list: D017607:Aluminum Compounds; D000890:Anti-Infective Agents; D017610:Calcium Compounds; D003470:Culture Media; D004338:Drug Combinations; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; C086631:mineral trioxide aggregate; D017641:Zeolites; D012834:Silver