title: Label-free, electrochemical detection of methicillin-resistant Staphylococcus aureus DNA with reduced graphene oxide-modified electrodes.
abstract: Reduced graphene oxide (rGO)-modified glassy carbon electrode is used to detect the methicillin-resistant Staphylococcus aureus (MRSA) DNA by using electrochemical impedance spectroscopy. Our experiments confirm that ssDNA, before and after hybridization with target DNA, are successfully anchored on the rGO surface. After the probe DNA, pre-adsorbed on rGO electrode, hybridizes with target DNA, the measured impedance increases dramatically. It provides a new method to detect DNA with high sensitivity (10(-13)M, i.e., 100 fM) and selectivity.
authors: School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore.
authors: Zhijuan
authors: Z
authors: Wang
authors: School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore.
authors: Juan
authors: J
authors: Zhang
authors: School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore; Center for Biomimetic Sensor Science, Nanyang Technological University, 50 Nanyang Drive, Singapore 637553, Singapore.
authors: Peng
authors: P
authors: Chen
authors: School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore.
authors: Xiaozhu
authors: X
authors: Zhou
authors: School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore; Center for Biomimetic Sensor Science, Nanyang Technological University, 50 Nanyang Drive, Singapore 637553, Singapore.
authors: Yanli
authors: Y
authors: Yang
authors: School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore.
authors: Shixin
authors: S
authors: Wu
authors: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
authors: Li
authors: L
authors: Niu
authors: Division of Chemical and Life Science and Engineering, Membrane Research Center, King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia.
authors: Yu
authors: Y
authors: Han
authors: Laboratory of Advanced Materials, Fudan University, Shanghai 200438, China.
authors: Lianhui
authors: L
authors: Wang
authors: School of Chemical and Biomedical Engineering, Nanyang Technological University, 70 Nanyang Drive, Singapore 637457, Singapore.
authors: Peng
authors: P
authors: Chen
authors: School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore; Center for Biomimetic Sensor Science, Nanyang Technological University, 50 Nanyang Drive, Singapore 637553, Singapore.
authors: Freddy
authors: F
authors: Boey
authors: School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore.
authors: Qichun
authors: Q
authors: Zhang
authors: School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore; Center for Biomimetic Sensor Science, Nanyang Technological University, 50 Nanyang Drive, Singapore 637553, Singapore.
authors: Bo
authors: B
authors: Liedberg
authors: School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore; Center for Biomimetic Sensor Science, Nanyang Technological University, 50 Nanyang Drive, Singapore 637553, Singapore.
authors: Hua
authors: H
authors: Zhang
mesh_terms: D015374:Biosensing Techniques; D002244:Carbon / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification*; D058266:Dielectric Spectroscopy; D006108:Graphite / Q000737:chemistry; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000737:chemistry / Q000302:isolation & purification*; D010087:Oxides / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D010087:Oxides; D002244:Carbon; D006108:Graphite