title: Utility of the discriminant analysis to categorize untrained Spanish Pure Bred horses on the basis of biochemical and muscle variables before and after a standardized exercise test.
abstract: We hypothesized that the information obtained from a discriminant analysis could be used to objectively discriminate horses untrained from early ages, in agreement with certain physiological characteristics. In the biopsies of 24 Spanish Pure Bred horses (1.5-3 years old) before and after a standardized exercise test (SET; 4-7 m/s with a change of velocity of 1m/s every 2 min) muscle enzymes, substrate and metabolites were determined. Also, diverse plasma and blood parameters were considered. Three pre-exercise groups (A1: six horses; A2: seven horses and A3: eleven horses) and two post-exercise groups (B1: sixteen horses; B2: eight horses) were defined from a correspondence analysis. Forward stepwise discriminant analysis selected 11 variables which differentiated the groups between each other both pre- and post-exercise. The results of the present study suggested the utility of a discriminant analysis to categorize horses in agreement with certain physiological variables. It could be used for establishing different types of training in each group by expert trainers.
authors: Department of Cell Biology, Physiology and Immunology, Section of Animal Physiology, Campus de Rabanales, Córdoba University, 14071 Córdoba, Spain. am1esdub@uco.es
authors: B M
authors: BM
authors: Escribano
authors: E I
authors: EI
authors: Agüera
authors: M D
authors: MD
authors: Rubio
authors: R
authors: R
authors: Santisteban
authors: R
authors: R
authors: Vivo
authors: S
authors: S
authors: Agüera
authors: P
authors: P
authors: Tovar
mesh_terms: D000818:Animals; D016002:Discriminant Analysis; D005080:Exercise Test; D006736:Horses / Q000097:blood / Q000502:physiology*; D008297:Male; D018482:Muscle, Skeletal / Q000502:physiology*; D010805:Physical Conditioning, Animal / Q000502:physiology*