title: Proteomic analysis of human mesenchymal stem cells.
abstract: Umbilical cord (UC) and placenta (P) are generally believed to be potential alternatives to bone marrow (BM), as sources of mesenchymal stem cells (MSC) for cell therapy. They possess immunophenotypic and functional characteristics which are similar to that of BM-MSC, yet one of the crucial factors in determining the tissue regeneration process--the migration capacity--is still unclear. In our previous study, the migration capacity of BM- and P-MSC was found 5.9- and 3.2-fold higher than that of UC-MSC, respectively. By using 2-dimensional gel electrophoresis (2-DE) and combined MS and MS/MS analysis, six proteins were identified as differentially expressed among these MSC samples. Five out of the six proteins were known to be involved in cell migration as migration inhibiting or enhancing proteins.
authors: Stanley Ho Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Hong Kong, PR China.
authors: Guo
authors: G
authors: Li
authors: Chu-yan
authors: CY
authors: Chan
authors: Hua
authors: H
authors: Wang
authors: Hsiang-fu
authors: HF
authors: Kung
mesh_terms: D050156:Adipogenesis; D015153:Blotting, Western; D001854:Bone Marrow Cells / Q000166:cytology; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002465:Cell Movement; D002469:Cell Separation; D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D010012:Osteogenesis; D010920:Placenta / Q000378:metabolism; D011247:Pregnancy; D014176:Protein Biosynthesis; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry; D014357:Trypsin / Q000378:metabolism; D014470:Umbilical Cord / Q000378:metabolism
chemical_list: D014357:Trypsin