title: Heme oxygenase-1 is induced by thyroid hormone and involved in thyroid hormone preconditioning-induced protection against renal warm ischemia in rat.
abstract: Thyroid hormone pretreatment was indicated to increase tissue tolerance to ischemia-reperfusion injury (IRI) in various organs, but the underlying molecular mechanisms remains largely unknown. Induction of heme oxygenase-1 (HO-1) protects organs against IRI. The present study investigated the effect of thyroid hormone on HO-1 expression and the possible relation between HO-1 and the thyroid hormone induced renal protection. T(3) administration in rat kidneys induced HO-1 expression in a time-dependent and dose-dependent way, and its expression was accompanied with significant depletion of reduced glutathione and increase in malondialdehyde content, showing a moderate oxidative stress that turns to normal level 48 h after drug injection. Thyroid hormone pretreatment (10 μg/100g body weight) 48 h before IR procedure significantly decreased serum creatinine and urea nitrogen and preserved renal histology, with significant reduction of parameters about oxidative stress and over-expression of HO-1 compared with that of IR group. In conclusion, T(3) administration involving oxidative stress in kidney exerts significant enhancement of HO-1 expression which may, at least in part, account for the renal preconditioning induced by T(3).
authors: Central Laboratory, Changzhou NO.2 hospital affiliated to Nanjing Medical University, Changzhou 213000, PR China.
authors: Fei
authors: F
authors: Li
authors: Shuyan
authors: S
authors: Lu
authors: Ruixia
authors: R
authors: Zhu
authors: Zhongxin
authors: Z
authors: Zhou
authors: Lingdi
authors: L
authors: Ma
authors: Leiming
authors: L
authors: Cai
authors: Zhiyuan
authors: Z
authors: Liu
mesh_terms: D000818:Animals; D015870:Gene Expression; D005978:Glutathione / Q000378:metabolism; D051547:Heme Oxygenase-1 / Q000235:genetics / Q000378:metabolism*; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism / Q000503:physiopathology; D007684:Kidney Tubules / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D014284:Triiodothyronine / Q000097:blood / Q000494:pharmacology*
chemical_list: D014284:Triiodothyronine; D008315:Malondialdehyde; D051547:Heme Oxygenase-1; D005978:Glutathione