title: Dopamine signalling in mushroom bodies regulates temperature-preference behaviour in Drosophila.
abstract: The ability to respond to environmental temperature variation is essential for survival in animals. Flies show robust temperature-preference behaviour (TPB) to find optimal temperatures. Recently, we have shown that Drosophila mushroom body (MB) functions as a center controlling TPB. However, neuromodulators that control the TPB in MB remain unknown. To identify the functions of dopamine in TPB, we have conducted various genetic studies in Drosophila. Inhibition of dopamine biosynthesis by genetic mutations or treatment with chemical inhibitors caused flies to prefer temperatures colder than normal. We also found that dopaminergic neurons are involved in TPB regulation, as the targeted inactivation of dopaminergic neurons by expression of a potassium channel (Kir2.1) induced flies with the loss of cold avoidance. Consistently, the mutant flies for dopamine receptor gene (DopR) also showed a cold temperature preference, which was rescued by MB-specific expression of DopR. Based on these results, we concluded that dopamine in MB is a key component in the homeostatic temperature control of Drosophila. The current findings will provide important bases to understand the logic of thermosensation and temperature preference decision in Drosophila.
authors: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Taejon, Korea.
authors: Sunhoe
authors: S
authors: Bang
authors: Seogang
authors: S
authors: Hyun
authors: Sung-Tae
authors: ST
authors: Hong
authors: Jongkyun
authors: J
authors: Kang
authors: Kyunghwa
authors: K
authors: Jeong
authors: Joong-Jean
authors: JJ
authors: Park
authors: Joonho
authors: J
authors: Choe
authors: Jongkyeong
authors: J
authors: Chung
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000502:physiology*; D001833:Body Temperature Regulation / Q000235:genetics; D001921:Brain / Q000378:metabolism; D003080:Cold Temperature; D004298:Dopamine / Q000378:metabolism*; D004330:Drosophila / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D005786:Gene Expression Regulation / Q000235:genetics; D024521:Mushroom Bodies / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D009474:Neurons / Q000378:metabolism; D011954:Receptors, Dopamine / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D011954:Receptors, Dopamine; D004298:Dopamine