title: Use of the loop-mediated isothermal amplification method for identification of PCR ribotype 027 Clostridium difficile.
abstract: The loop-mediated isothermal amplification (LAMP) assay detecting the slpA gene of slpA sequence type gc8 (slpA-gc8) was established for the identification of a hypervirulent Clostridium difficile strain, PCR ribotype 027. Of 107 isolates examined, 27 belonging to PCR ribotype 027 were all positive for the LAMP assay. The remaining 80 isolates were typed into 47 different PCR ribotypes other than type 027, and were negative for the LAMP assay with the exception of two isolates. The sensitivity and specificity of the LAMP method for identification of PCR ribotype 027 were 100 % and 98 %, respectively. The LAMP assay detecting slpA-gc8 is a reliable tool for the identification of PCR ribotype 027 C. difficile. This simple and rapid method will contribute to detection of the hypervirulent strain.
authors: Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo, Japan.
authors: Haru
authors: H
authors: Kato
authors: Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo, Japan.
authors: Yoshichika
authors: Y
authors: Arakawa
mesh_terms: D016360:Clostridioides difficile / Q000145:classification* / Q000235:genetics*; D046508:Culture Techniques; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D021521:Ribotyping