title: Fast-forward genetics enabled by new sequencing technologies.
abstract: New sequencing technologies are dramatically accelerating progress in forward genetics, and the use of such methods for the rapid identification of mutant alleles will be soon routine in many laboratories. A straightforward extension will be the cloning of major-effect genetic variants in crop species. In the near future, it can be expected that mapping by sequencing will become a centerpiece in efforts to discover the genes responsible for quantitative trait loci. The largest impact, however, might come from the use of these strategies to extract genes from non-model, non-crop plants that exhibit heritable variation in important traits. Deployment of such genes to improve crops or engineer microbes that produce valuable compounds heralds a potential paradigm shift for plant biology.
authors: Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tübingen, Germany.
authors: Korbinian
authors: K
authors: Schneeberger
authors: Detlef
authors: D
authors: Weigel
mesh_terms: D000483:Alleles; D002874:Chromosome Mapping / Q000266:history / Q000379:methods*; D018556:Crops, Agricultural / Q000235:genetics; D004247:DNA / Q000266:history / Q000302:isolation & purification; D014644:Genetic Variation; D005838:Genotype; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D049672:History, 19th Century; D049673:History, 20th Century; D008967:Molecular Biology / Q000379:methods; D009154:Mutation; D010641:Phenotype; D010944:Plants / Q000235:genetics*; D040641:Quantitative Trait Loci / Q000235:genetics
chemical_list: D004247:DNA