title: Spatiotemporal fate map of neurogenin1 (Neurog1) lineages in the mouse central nervous system.
abstract: Neurog1 (Ngn1, Neurod3, neurogenin1) is a basic helix-loop-helix (bHLH) transcription factor essential for neuronal differentiation and subtype specification during embryogenesis. Due to the transient expression of Neurog1 and extensive migration of neuronal precursors, it has been challenging to understand the full complement of Neurog1 lineage cells throughout the central nervous system (CNS). Here we labeled and followed Neurog1 lineages using inducible Cre-flox recombination systems with Neurog1-Cre and Neurog1-CreER(T2) BAC (bacterial artificial chromosome) transgenic mice. Neurog1 lineage cells are restricted to neuronal fates and contribute to diverse but discrete populations in each brain region. In the forebrain, Neurog1 lineages include mitral cells and glutamatergic interneurons in the olfactory bulb, pyramidal and granule neurons in the hippocampus, and pyramidal cells in the cortex. In addition, most of the thalamus, but not the hypothalamus, arises from Neurog1 progenitors. Although Neurog1 lineages are largely restricted to glutamatergic neurons, there are multiple exceptions including Purkinje cells and other GABAergic neurons in the cerebellum. This study provides the first overview of the spatiotemporal fate map of Neurog1 lineages in the CNS.
authors: Department of Neuroscience, UT Southwestern Medical Center, Dallas, Texas 75390-9111, USA.
authors: Euiseok J
authors: EJ
authors: Kim
authors: Kei
authors: K
authors: Hori
authors: Alex
authors: A
authors: Wyckoff
authors: Lauren K
authors: LK
authors: Dickel
authors: Edmund J
authors: EJ
authors: Koundakjian
authors: Lisa V
authors: LV
authors: Goodrich
authors: Jane E
authors: JE
authors: Johnson
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism*; D001931:Brain Mapping; D002454:Cell Differentiation / Q000502:physiology; D019070:Cell Lineage; D002490:Central Nervous System / Q000033:anatomy & histology* / Q000378:metabolism*; D004965:Estrogen Antagonists / Q000494:pharmacology; D005260:Female; D017403:In Situ Hybridization; D051379:Mice; D008822:Mice, Transgenic; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D011247:Pregnancy; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism; D013629:Tamoxifen / Q000494:pharmacology
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D004965:Estrogen Antagonists; D009419:Nerve Tissue Proteins; D013629:Tamoxifen; C486336:Neurog1 protein, mouse