title: Molecular recognition force spectroscopy: a new tool to tailor targeted nanoparticles.
abstract: The density of targeting moieties in a nanoparticle-based gene-delivery system has been shown to play a fundamental role in its vectoring performance. Here, molecular recognition force spectroscopy is proposed as a novel screening tool to optimize the density of targeting moieties of functionalized nanoparticles towards attaining cell-specific interaction. By tailoring the nanoparticle formulation, the unbinding event probability between nanoparticles tethered to an atomic force microscopy tip and neuronal cells is directly correlated to the nanoparticle gene-vectoring capacity. Additionally, new insights into protein-receptor interaction are revealed. This novel approach opens new avenues in the field of nanomedicine.
authors: INEB-Instituto de Engenharia Biomédica, Divisão de Biomateriais, Universidade do Porto, Rua do Campo Alegre 823, 4150-180 Porto, Portugal.
authors: Hugo
authors: H
authors: Oliveira
authors: Martina
authors: M
authors: Rangl
authors: Andreas
authors: A
authors: Ebner
authors: Barbara
authors: B
authors: Mayer
authors: Peter
authors: P
authors: Hinterdorfer
authors: Ana P
authors: AP
authors: Pêgo
mesh_terms: D018625:Microscopy, Atomic Force / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*