title: Ultraviolet photodissociation of chromophore-labeled oligosaccharides via reductive amination and hydrazide conjugation.
abstract: The fragmentation patterns of hydrazide-conjugated and reductively aminated oligosaccharides, including lacto-N-fucopentaoses and lacto-N-difucohexaoses, produced on collisionally induced dissociation (CID) and ultraviolet photodissociation (UVPD) in a quadrupole ion trap are presented. The two derivatization methods generate different cross-ring cleavages on UVPD and CID. UVPD of hydrazide-conjugated oligosaccharides yield predominant (2, 4)A-type cross-ring cleavage ions. In contrast, UVPD of aminated oligosaccharides results mainly in (0, 1)A-type ions. Moreover, more extensive dual-cleavage pathways (i.e. internal fragment ions) were observed on UVPD.
authors: Department Chemical Engineering, The University of Texas at Austin, 1 University Station A5300, Texas 78712, USA.
authors: Byoung Joon
authors: BJ
authors: Ko
authors: Jennifer S
authors: JS
authors: Brodbelt
mesh_terms: D000586:Amination; D003374:Coumarins / Q000737:chemistry*; D006834:Hydrazines / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry*; D055668:Photochemical Processes; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014466:Ultraviolet Rays
chemical_list: D003374:Coumarins; D006834:Hydrazines; D009844:Oligosaccharides; C018991:lacto-N-difucohexaose I; C041762:lacto-N-fucopentaose II; C029424:hydrazine; C056146:lacto-N-difucohexose II; C045442:lacto-N-fucopentaose I; C028743:7-amino-4-methylcoumarin