title: Potential role of poly(ADP-ribose) polymerase activation in the pathogenesis of experimental left varicocele.
abstract: The aim of this study was to evaluate whether the poly(adenosine  diphosphate[ADP]-ribose) polymerase (PARP) pathway is activated by  experimental left varicocele. Rats underwent partial ligation of the left  renal vein to induce experimental varicocele, and left testes were analyzed 13  weeks after surgery. Tubule degeneration was evaluated by Johnsen score.  Expression of 4-hydroxy-2-nonenal (4-HNE)-modified proteins, PARP-1, and  poly(-ADP-ribose) (PAR) was detected by immunohistochemistry and Western blot.  The degree of apoptosis within testes was determined by terminal  deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end  labeling. Light microscopy revealed testicular damage comprising various  degrees of seminiferous tubule degeneration. Germ cell apoptotic index and  4-HNE, PAR, and PARP-1 expression in germ cells increased after varicocele  induction. Increased oxidative stress and PARP overactivation in testes might  be important with regard to impaired testicular function associated with  varicocele.
authors: Department of Histology and Embryology, Faculty of Medicine, Akdeniz University, Antalya, Turkey .
authors: Merih
authors: M
authors: Tekcan
authors: Ismail Turker
authors: IT
authors: Koksal
authors: Arda
authors: A
authors: Tasatargil
authors: Omer
authors: O
authors: Kutlu
authors: Ece
authors: E
authors: Gungor
authors: Ciler
authors: C
authors: Celik-Ozenci
mesh_terms: D000818:Animals; D015153:Blotting, Western; D004789:Enzyme Activation; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D008297:Male; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism*; D051381:Rats; D014646:Varicocele / Q000201:enzymology*
chemical_list: D011065:Poly(ADP-ribose) Polymerases