title: Transcriptional targeting of B cells with viral vectors.
abstract: In order to optimize viral gene transfer into hematopoietic stem cells we developed retroviral and lentiviral vectors with B cell-specificity. Using fragments of the human CD19 promoter we demonstrate in mice that upon lethal irradiation and reconstitution with virus-treated bone marrow transgene expression is specific for the B cell-lineage. We compare various viral constructs with different promoter length and with or without B cell-specific enhancer regions in retro- and lentiviral backbones. Our data suggest that B cell-targeting for gene therapy approaches is feasible, leads to stable expression, and can be modulated by using different transduction and expression systems.
authors: Institute for Immunology, Ludwig-Maximilians-University, Goethestrasse 31, Munich, Germany.
authors: Romana I
authors: RI
authors: Friedrich
authors: Katrin
authors: K
authors: Nopora
authors: Thomas
authors: T
authors: Brocker
mesh_terms: D000818:Animals; D018941:Antigens, CD19 / Q000235:genetics / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D001853:Bone Marrow / Q000276:immunology / Q000528:radiation effects; D005434:Flow Cytometry; D005720:Gamma Rays; D015870:Gene Expression; D005786:Gene Expression Regulation / Q000276:immunology; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology / Q000528:radiation effects; D006801:Humans; D016086:Lentivirus / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D058990:Molecular Targeted Therapy / Q000379:methods*; D009928:Organ Specificity; D011401:Promoter Regions, Genetic / Q000276:immunology; D012190:Retroviridae / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D014161:Transduction, Genetic; D019076:Transgenes; D014916:Whole-Body Irradiation / Q000009:adverse effects
chemical_list: D018941:Antigens, CD19; D049452:Green Fluorescent Proteins