title: Modulation of collagen II fiber formation in 3-D porous scaffold environments.
abstract: Collagen II, a major extracellular matrix component in cartilaginous tissues, undergoes fibrillogenesis under physiological conditions. The present study explored collagen II fiber formation in solution and in two- (coverslip) and three-dimensional (scaffold) environments under different incubation conditions. These conditions include variations in adsorption buffers, the presence of 1-ethyl-3-(3-dimenthylaminopropyl) carbodiimide/N-hydroxysuccinimide crosslinker and the nature of the material surfaces. We extend our observations of collagen II fiber formation in two dimensions to develop an approach for the formation of a fibrillar collagen II network throughout surface-modified polylactide-co-glycolide porous scaffolds. Morphologically, the collagen II network is similar to that present in native articular cartilage. Biological validation of the resultant optimized functional scaffold, using rat bone marrow-derived mesenchymal stem cells, shows appreciable cell infiltration throughout the scaffold with enhanced cell spreading at 24h post-seeding. This economic and versatile approach is thus believed to have significant potential in cartilage tissue engineering applications.
authors: Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Bld 75, Cnr Cooper and College Rds, QLD 4072, Australia.
authors: Guak-Kim
authors: GK
authors: Tan
authors: Donna Lee M
authors: DL
authors: Dinnes
authors: Justin J
authors: JJ
authors: Cooper-White
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D002358:Cartilage, Articular / Q000737:chemistry / Q000166:cytology; D002417:Cattle; D002478:Cells, Cultured; D024043:Collagen Type II / Q000737:chemistry* / Q000378:metabolism* / Q000648:ultrastructure; D005109:Extracellular Matrix / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology; D015394:Molecular Structure; D016062:Porosity; D011487:Protein Conformation; D051381:Rats; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D024043:Collagen Type II