title: Asperosaponin VI, a saponin component from Dipsacus asper wall, induces osteoblast differentiation through bone morphogenetic protein-2/p38 and extracellular signal-regulated kinase 1/2 pathway.
abstract: Osteoporosis is a reduction in skeletal mass because of the loss of osteoblastic activity or an increase in osteoclastic activity. The survival of osteoblast cells plays a crucial role in the development of osteoporosis. Asperosaponin VI (ASA VI) is a kind of saponin in the medicinal herb Dipsacus asper Wall which has long been used as an antiosteoporosis drug. The assay of cell proliferation, alkaline phosphatase (ALP) activity and measurement of mineralized matrix, showed that ASA VI exhibited a significant induction of proliferation, differentiation and mineralization in MC3T3-E1 and primary osteoblastic cells. Induction of differentiation by ASA VI was associated with increased bone morphogenetic protein-2 (BMP-2), indicating that BMP-2 is essential in ASA VI to mediate osteoblast maturation and differentiation. In addition, ASA VI may induce differentiation by increasing the activity of p38 and ERK1/2. In conclusion, ASA VI may induce osteoblast maturation and differentiation, and then increase bone formation via increasing BMP-2 synthesis, and activating p38 and ERK1/2.
authors: Faculty of Life Sciences, Northwestern Polytechnical University, Shaanxi, China.
authors: Yinbo
authors: Y
authors: Niu
authors: Yuhua
authors: Y
authors: Li
authors: Haitao
authors: H
authors: Huang
authors: Xianghe
authors: X
authors: Kong
authors: Rong
authors: R
authors: Zhang
authors: Li
authors: L
authors: Liu
authors: Yang
authors: Y
authors: Sun
authors: Tingmei
authors: T
authors: Wang
authors: Qibing
authors: Q
authors: Mei
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism*; D002113:Calcification, Physiologic / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D049109:Cell Proliferation; D049917:Dipsacaceae / Q000737:chemistry; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D051379:Mice; D010006:Osteoblasts / Q000166:cytology*; D051381:Rats; D012503:Saponins / Q000494:pharmacology*
chemical_list: C525719:Bmp2 protein, mouse; D055396:Bone Morphogenetic Protein 2; D012503:Saponins; C534004:akebia saponin D; D000469:Alkaline Phosphatase