title: Immunoglobulin g (IgG) expression in human umbilical cord endothelial cells.
abstract: Traditional views hold that immunoglobulin G (IgG) in the human umbilical cord is internalized by human umbilical endothelial cells for passive immunity. In this study, the protein and mRNA transcripts of IgG were found in the cytoplasm of human umbilical endothelial cells by immunohistochemistry, in situ hybridization, and reverse transcription PCR (RT-PCR). The essential enzymes for IgG synthesis and assembling, RAG1 (recombination activating gene 1), RAG2, and variable (V), diversity (D), and joining (J) segments for recombination of IgG, were also found in these cells by RT-PCR and real-time PCR. These results indicate that umbilical endothelial cells are capable of synthesizing IgG with properties similar to those of immune cells and that they may play additional roles besides lining the vessels and transporting IgG.
authors: Department of Pathology, School of Basic Medical Sciences, Peking University, Beijing, China.
authors: Yingying
authors: Y
authors: Zhao
authors: Yuxuan
authors: Y
authors: Liu
authors: Zhengshan
authors: Z
authors: Chen
authors: Christine
authors: C
authors: Korteweg
authors: Jiang
authors: J
authors: Gu
mesh_terms: D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics; D042783:Endothelial Cells / Q000378:metabolism*; D015395:Histocompatibility Antigens Class I / Q000378:metabolism; D018398:Homeodomain Proteins / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis* / Q000235:genetics; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D009687:Nuclear Proteins / Q000096:biosynthesis / Q000235:genetics; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D011961:Receptors, Fc / Q000378:metabolism; D014470:Umbilical Cord / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D015395:Histocompatibility Antigens Class I; D018398:Homeodomain Proteins; D007074:Immunoglobulin G; D009687:Nuclear Proteins; C499311:RAG2 protein, human; D012333:RNA, Messenger; D011961:Receptors, Fc; C064658:RAG-1 protein; C100688:Fc receptor, neonatal