title: Capillary electrophoresis with contactless conductivity detection for the determination of carnitine and acylcarnitines in clinical samples.
abstract: A capillary electrophoresis method with contactless conductivity detection was developed for the quantification of carnitine and six acylcarnitines in plasma and urine samples. The running buffer employed consisted of 500 mmol/L acetic acid, 1.0 mmol/L hydroxypropyl-β-cyclodextrin and 0.05% Tween at a pH of 2.6. Under these conditions, the isomeric valproyl- and octanoyl-carnitines could be distinguished. The linearity was in the range from 5.0 to 200.0 μmol/L with correlation coefficients between 0.9992 and 0.9997. The limits of detection were between 1.0 and 3.2 μmol/L. Intra- and inter-day precisions as %RSD were better than 10%. The method allows for direct determination without derivatisation or extraction processes. The method was applied for the quantification of carnitine and acetylcarnitine in plasma pre- and post-exercise, and to measure valproylcarnitine in plasma and urine of patients undergoing valproate therapy.
authors: Department of Chemistry, University of Basel, Basel, Switzerland.
authors: Worapan
authors: W
authors: Pormsila
authors: Réjane
authors: R
authors: Morand
authors: Stephan
authors: S
authors: Krähenbühl
authors: Peter C
authors: PC
authors: Hauser
mesh_terms: D002331:Carnitine / Q000031:analogs & derivatives* / Q000032:analysis* / Q000097:blood / Q000652:urine; D004553:Electric Conductivity; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D016014:Linear Models; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction
chemical_list: C116917:acylcarnitine; D002331:Carnitine