title: Isolation and partial characterization of excretory/secretory antigens of Gastrothylax crumenifer.
abstract: The present study was carried out to identify the excretory/secretory (E/S) antigens of the rumen infecting digenetic trematode Gastrothylax crumenifer that may be useful for the immunodiagnosis of rumen amphistomosis particularly during the pre-monsoon season during which this rumen parasite stops shedding eggs. The in vitro released E/S proteins were purified on a Sephadex G-200 column. The gel filtration profile revealed three distinct fractions F1-F3 where F1 and F3 appeared as sharp peaks while the F2 fraction was dispersed. The antibody titre against each of the purified E/S fractions was determined by ELISA using anti-whole E/S polyclonal antibodies raised in rabbit. Among the three fractions, the antibody titre against F1 was highest (1:12,800) whereas IgG titre was very low (1:50) for fraction F2 and F3 (1:100). Of the total polypeptides resolved on gradient SDS-PAGE, only a few antigenic polypeptides were detected in each fraction with hyperimmune anti-serum as revealed by Western Blot analysis. However, a 33 kDa antigen detected in each fraction appeared to be immunodominant which could be exploited for the diagnosis of the pouched amphistome.
authors: Section of Parasitology, Department of Zoology, Aligarh Muslim University, Aligarh 202002, India. mkhalidsaif@yahoo.com
authors: Mohammad K
authors: MK
authors: Saifullah
authors: Gul
authors: G
authors: Ahmad
authors: Syed M A
authors: SM
authors: Abidi
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology / Q000378:metabolism*; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D011817:Rabbits; D014200:Trematoda / Q000276:immunology / Q000378:metabolism*
chemical_list: D000947:Antigens, Helminth