title: Sub-cellular internalization and organ specific oral delivery of PABA nanoparticles by side chain variation.
abstract: BACKGROUND
  Organic nanomaterials having specific biological properties play important roles in in vivo delivery and clearance from the live cells. To develop orally deliverable nanomaterials for different biological applications, we have synthesized several fluorescently labelled, self-assembled PABA nanoparticles using possible acid side chain combinations and tested against insect and human cell lines and in vivo animal model. Flurophores attached to nanostructures help in rapid in vivo screening and tracking through complex tissues. The sub-cellular internalization mechanism of the conjugates was determined. A set of physio-chemical parameters of engineered nanoskeletons were also defined that is critical for preferred uptake in multiple organs of live Drosophila.
  
  
  RESULTS
  The variability of side chains alter size, shape and surface texture of each nanomaterial that lead to differential uptake in human and insect cells and to different internal organs in live Drosophila via energy dependent endocytosis. Our results showed that physical and chemical properties of C-11 and C-16 acid chain are best fitted for delivery to complex organs in Drosophila. However a distinct difference in uptake of same nanoparticle in human and insect cells postulated that different host cell physiology plays a critical role in the uptake mechanism.
  
  
  CONCLUSIONS
  The physical and chemical properties of the nanoparticle produced by variation in the acid side chains that modify size and shape of engineered nanostructure and their interplay with host cell physiology might be the major criteria for their differential uptake to different internal organs.
authors: Indian Institute of Chemical Technology Uppal Road, Hyderbad-500007, India. yadav@iict.res.in
authors: Jhillu S
authors: JS
authors: Yadav
authors: Pragna P
authors: PP
authors: Das
authors: T Lakshminarayan
authors: TL
authors: Reddy
authors: Indira
authors: I
authors: Bag
authors: Priyadarshini M
authors: PM
authors: Lavanya
authors: Bulusu
authors: B
authors: Jagannadh
authors: Debendra K
authors: DK
authors: Mohapatra
authors: Manika Pal
authors: MP
authors: Bhadra
authors: Utpal
authors: U
authors: Bhadra
mesh_terms: D010129:4-Aminobenzoic Acid / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics*; D000284:Administration, Oral; D000818:Animals; D002460:Cell Line; D004330:Drosophila / Q000378:metabolism; D004705:Endocytosis; D006801:Humans; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry
chemical_list: D010129:4-Aminobenzoic Acid