title: Nanoscale chemical imaging of segregated lipid domains using tip-enhanced Raman spectroscopy.
abstract: Lipid domains in supported lipid layers serve as a popular model to gain insight into the processes associated with the compartmentalization of biological membranes into so-called lipid rafts. In this paper, we present reproducible tip-enhanced Raman spectra originating from a very small number of molecules in a lipid monolayer on a gold surface, probed by the apex of a nanometer-sized silver tip. For the first time, we show large (128 × 128 pixels), high-resolution (< 50 nm) tip-enhanced Raman images of binary lipid mixtures with full spectral information at each pixel.
authors: Department of Chemistry and Applied Biosciences, ETH Zurich, CH-8093 Zurich, Switzerland.
authors: Lothar
authors: L
authors: Opilik
authors: Thomas
authors: T
authors: Bauer
authors: Thomas
authors: T
authors: Schmid
authors: Johannes
authors: J
authors: Stadler
authors: Renato
authors: R
authors: Zenobi
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry; D008055:Lipids / Q000032:analysis*; D021962:Membrane Microdomains / Q000737:chemistry*; D008567:Membranes, Artificial; D018625:Microscopy, Atomic Force; D010713:Phosphatidylcholines / Q000737:chemistry; D012834:Silver / Q000737:chemistry*; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D008055:Lipids; D008567:Membranes, Artificial; D010713:Phosphatidylcholines; D015060:1,2-Dipalmitoylphosphatidylcholine; D012834:Silver; C017251:1,2-oleoylphosphatidylcholine