title: Bactofection with toll-like receptor 4 in a murine model of urinary tract infection.
abstract: The role of innate immunity in the prevention of urinary tract infection is well-documented. Toll-like receptor 4 (TLR4) is a major determinant of innate immune response. In an animal model of urinary tract infection, bactofection-mediated gene transfer of TLR4 was tested in a preventive approach. Bactofection with TLR4 reduced the colonization with uropathogenic Escherichia coli by 91% in the kidney and by 41% in the bladder. Reduced colonization was associated with lower oxidative stress and expression of monocyte chemoattractant protein-1 and myeloperoxidase in the kidney. Bactofection with TLR4 was successful in the prevention of ascending pyelonephritis. Further studies should focus on long-term effects, the dose response and the potential therapeutic use in models of chronic urinary tract infection.
authors: Institute of Molecular Biomedicine, Comenius University, Sasinkova 4, 811 08 Bratislava, Slovakia.
authors: Lubomíra
authors: L
authors: Tóthová
authors: Július
authors: J
authors: Hodosy
authors: Natália
authors: N
authors: Kamodyová
authors: Pavol
authors: P
authors: Janega
authors: Lívia
authors: L
authors: Slobodníková
authors: Adriana
authors: A
authors: Liptáková
authors: Peter
authors: P
authors: Boor
authors: Peter
authors: P
authors: Celec
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004927:Escherichia coli Infections / Q000235:genetics* / Q000276:immunology / Q000382:microbiology / Q000628:therapy*; D005260:Female; D015316:Genetic Therapy; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D011897:Random Allocation; D051197:Toll-Like Receptor 4 / Q000235:genetics* / Q000276:immunology; D014162:Transfection; D014552:Urinary Tract Infections / Q000235:genetics* / Q000276:immunology / Q000382:microbiology / Q000628:therapy*; D056689:Uropathogenic Escherichia coli / Q000235:genetics / Q000276:immunology*
chemical_list: D051197:Toll-Like Receptor 4