title: Effect of chlorhexidine pretreatment on the stability of salivary lactobacilli probiotic in six- to twelve-year-old children: a randomized controlled trial.
abstract: This study aimed to investigate the efficacy of chlorhexidine mouthwash disinfection on the stability of lactobacilli probiotic. This study involved 105 children in 3 groups. Subjects in group A received chlorhexidine for 2 weeks, subjects of group B received probiotic yogurt containing Lactobacillus rhamnosus GG (LGG) at a concentration of 2 × 10(8) colony-forming units per gram for 3 weeks, and subjects in group C received chlorhexidine in the same manner as group A and 24 h after cessation, they received probiotic yogurt in the same manner as group B. Salivary Streptococcus mutans (SM) and LGG were counted at baseline, 24 h, 1 week and 5 weeks after each intervention in all groups. The SM count was reduced 24 h after cessation of chlorhexidine in group A (p < 0.001), and was steady for 5 weeks (p < 0.05). In group B, SM counts did not show significant differences 5 weeks after ending the intake of the probiotic (p > 0.05); LGG counts increased (p < 0.001), but were not steady over 5 weeks. In group C, a significant reduction in SM count was recorded, and the reduced count was steady for 5 weeks; a significant increase in LGG counts was registered (p < 0.001), which was steady for 5 weeks. Intergroup comparison showed a significant increase in LGG counts in groups B and C; however, this increase was not steady in group B. Chlorhexidine treatment before probiotic intake induced more stable colonization with LGG strains than probiotic alone.
authors: Department of Pediatric Dentistry, Tabriz University of Medical Sciences, Iran. aslaminabadi@gmail.com
authors: N A
authors: NA
authors: Aminabadi
authors: L
authors: L
authors: Erfanparast
authors: A
authors: A
authors: Ebrahimi
authors: S G
authors: SG
authors: Oskouei
mesh_terms: D000704:Analysis of Variance; D019072:Antibiotic Prophylaxis; D002648:Child; D002710:Chlorhexidine / Q000008:administration & dosage / Q000494:pharmacology*; D015169:Colony Count, Microbial; D003731:Dental Caries / Q000517:prevention & control; D005260:Female; D006801:Humans; D052201:Lacticaseibacillus rhamnosus / Q000254:growth & development*; D008297:Male; D056265:Microbial Interactions / Q000187:drug effects*; D009067:Mouthwashes / Q000008:administration & dosage / Q000494:pharmacology*; D019936:Probiotics / Q000627:therapeutic use*; D012463:Saliva / Q000382:microbiology; D018709:Statistics, Nonparametric; D013295:Streptococcus mutans / Q000187:drug effects*; D015014:Yogurt / Q000382:microbiology
chemical_list: D009067:Mouthwashes; D002710:Chlorhexidine