title: VEGF-A not Ang2 mediates endothelial-like differentiation of immature DCs by ERK1/2 signaling in the microenvironment of human colon adenocarcinoma.
abstract: Endothelial-like differentiation of dendritic cells (DCs) is an interesting and significant phenomenon, which is worth further investigation. Here, we show that the tumor microenvironment derived from the supernatant of the SW620 human colon adenocarcinoma cell line and colon adenocarcinoma tissue homogenate can promote immature DCs (iDCs) to differentiate from the DC pathway toward endothelial cells, while the peri-carcinoma homogenate supernatant does not have this role. Inhibition of angiopoietin-2 (Ang2) in the supernatant by its antibody has no obvious influence on the endothelial-like differentiation. In contrast, inhibition of vascular endothelial growth factor-A (VEGF-A) blocked the differentiation. During the course of differentiation, a sustained activation of ERK1/2 was detected. PD98059 blocked the phosphorylation of ERK1/2 as well as the endothelial-like differentiation of iDCs. Inhibition of VEGF-A also blocked the phosphorylation of ERK1/2. These data suggest that VEGF-A not Ang2 mediates endothelial-like differentiation of iDCs by ERK1/2 signaling in the microenvironment of human colon adenocarcinoma.
authors: Department of Pathophysiology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001, PR China.
authors: Jing
authors: J
authors: Lu
authors: Kangdong
authors: K
authors: Liu
authors: Jimin
authors: J
authors: Zhao
authors: Jun
authors: J
authors: Zhao
authors: Junfen
authors: J
authors: Ma
authors: Hongyan
authors: H
authors: Yang
authors: Youtian
authors: Y
authors: Huang
authors: Zhenzhu
authors: Z
authors: Qin
authors: Ruihua
authors: R
authors: Bai
authors: Lili
authors: L
authors: Jiang
authors: Fengshou
authors: F
authors: Lv
authors: Pei
authors: P
authors: Li
authors: Wenhai
authors: W
authors: Yan
authors: Mingyao
authors: M
authors: Zhao
authors: Ziming
authors: Z
authors: Dong
mesh_terms: D000230:Adenocarcinoma / Q000503:physiopathology*; D042702:Angiopoietin-2 / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003110:Colonic Neoplasms / Q000503:physiopathology*; D017077:Culture Media, Conditioned; D003713:Dendritic Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D042783:Endothelial Cells / Q000378:metabolism; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D005419:Flavonoids / Q000494:pharmacology; D006801:Humans; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects; D059016:Tumor Microenvironment; D042461:Vascular Endothelial Growth Factor A / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D042702:Angiopoietin-2; D017077:Culture Media, Conditioned; D005419:Flavonoids; D047428:Protein Kinase Inhibitors; D042461:Vascular Endothelial Growth Factor A; D048049:Extracellular Signal-Regulated MAP Kinases; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one