title: Alpha-ketoglutarate stabilizes redox homeostasis and improves arterial elasticity in aged mice.
abstract: The objective of this study was to evaluate the effect of α-ketoglutarate on redox state parameters and arterial elasticity in elderly mice. Mice in the control group were fed with standard diet, while the experimental animals received the diet supplemented either with calcium (Ca-AKG) or sodium salt of α-ketoglutarate (Na-AKG). The experimental animals were divided into 4 groups with 10 individuals in each: control I (12 months old), control II (2 months old), experimental group I fed with Ca-AKG (12 months old) and experimental group II fed with Na-AKG (12 months old). Mice treated with Ca-AKG as well as the control II animals demonstrated significantly higher level of total antioxidant status (TAS), comparing to the control I animals and those treated with Ca-AKG. Thiobarbituric acid reactive substances (TBARS) level in blood plasma was found significantly lower in young and Ca-AKG treated mice. TBARS liver concentration was significantly different in each examined group. The study also demonstrates the decrease in TBARS level in Ca-AKG treated animals. Treatment with Na-AKG significantly increased glutathione peroxidase activity and decreased the activity of superoxide dismutase. The presented results suggest that Ca-AKG protects the organism against the free radicals related elderly processes. The study presents also the effect of Ca-AKG treatment on arterial elastic characteristics in elderly mice. The beneficial effect of Ca-AKG on ageing organisms was confirmed via redox state stabilization and blood vessel elasticity improvement.
authors: Department of Animal Nutrition and Feed Sciences, Faculty of Animal Sciences, Warsaw University of Life Sciences, Warsaw, Poland. t_niemiec@tlen.pl
authors: T
authors: T
authors: Niemiec
authors: J
authors: J
authors: Sikorska
authors: A
authors: A
authors: Harrison
authors: M
authors: M
authors: Szmidt
authors: E
authors: E
authors: Sawosz
authors: E
authors: E
authors: Wirth-Dzieciolowska
authors: J
authors: J
authors: Wilczak
authors: S
authors: S
authors: Pierzynowski
mesh_terms: D000375:Aging / Q000187:drug effects*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001158:Arteries / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D002136:Calcium, Dietary / Q000494:pharmacology; D004032:Diet; D019587:Dietary Supplements; D004548:Elasticity / Q000187:drug effects; D005609:Free Radicals / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D006706:Homeostasis / Q000187:drug effects; D007656:Ketoglutaric Acids / Q000494:pharmacology*; D008099:Liver / Q000378:metabolism; D051379:Mice; D010084:Oxidation-Reduction; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000975:Antioxidants; D002136:Calcium, Dietary; D005609:Free Radicals; D007656:Ketoglutaric Acids; D017392:Thiobarbituric Acid Reactive Substances; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase