title: Selected acute phase CSF factors in ischemic stroke: findings and prognostic value.
abstract: BACKGROUND
  Study aimed at investigation of pathogenic role and prognostic value of several selected cerebrospinal fluid acute phase factors that can reflect the severity of ischemic brain damage.
  
  
  METHODS
  Ninety five acute ischemic stroke patients were investigated. Ischemic region visualized at the twenty fourth hour by conventional Magnetic Resonance Imaging. Stroke severity evaluated by National Institute Health Stroke Scale. One month outcome of disease was assessed by Barthel Index. Cerebrospinal fluid was taken at the sixth hour of stroke onset. CSF pro- and anti-inflammatory cytokines were studied by Enzyme Linked Immunosorbent Assay. Nitric Oxide and Lipoperoxide radical were measured by Electron Paramagnetic Resonance. CSF Nitrate levels were detected using the Griess reagent. Statistics performed by SPSS-11.0.
  
  
  RESULTS
  At the sixth hour of stroke onset, cerebrospinal fluid cytokine levels were elevated in patients against controls. Severe stroke patients had increased interleukin-6 content compared to less severe strokes (P < 0.05). Cerebrospinal fluid Electron Paramagnetic Resonance signal of nitric oxide was increased in patients against controls. Severe stroke group had an elevated Electron Paramagnetic Resonance signal of lipoperoxiradical compared to less severe stroke. Cerebrospinal fluid nitrate levels in less severe stroke patients were higher than those for severe stroke and control. Positive correlation was established between the initial interleukin-6 content and ischemic lesion size as well as with National Institute Health Stroke Scale score on the seventh day. Initial interleukin-6 and nitrate levels in cerebrospinal fluid found to be significant for functional outcome of stroke at one month.
  
  
  CONCLUSIONS
  According to present study the cerebrospinal fluid contents of interleukin-6 and nitrates seem to be the most reliable prognostic factors in acute phase of ischemic stroke.
authors: Department of Neuromedicine, Tbilisi State Medical University, Tbilisi, Georgia. postdoc2002@yahoo.com
authors: Maia
authors: M
authors: Beridze
authors: Tamar
authors: T
authors: Sanikidze
authors: Roman
authors: R
authors: Shakarishvili
authors: Nino
authors: N
authors: Intskirveli
authors: Natan M
authors: NM
authors: Bornstein
mesh_terms: D000209:Acute-Phase Proteins / Q000134:cerebrospinal fluid*; D000368:Aged; D015415:Biomarkers / Q000134:cerebrospinal fluid; D002545:Brain Ischemia / Q000134:cerebrospinal fluid* / Q000473:pathology; D004578:Electron Spin Resonance Spectroscopy; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D020521:Stroke / Q000134:cerebrospinal fluid* / Q000473:pathology
chemical_list: D000209:Acute-Phase Proteins; D015415:Biomarkers