title: Influence of experimental inflammatory response on hepatic hepcidin gene expression and plasma iron concentration in sheep.
abstract: Hepcidin is a highly conserved disulfide-bonded peptide that plays a central role in iron homeostasis. During systemic inflammation, hepcidin up-regulation is responsible for hypoferremia. This study aimed to analyze the influence of the inflammatory process induced by complete Freund's adjuvant (CFA) or lipopolysaccharide (LPS) on the liver expression of hepcidin mRNA transcripts and plasma iron concentration of sheep. The expression levels of hepcidin transcripts were up-regulated after CFA or LPS. Hypoferremic response was observed at 12 h (15.46 ± 6.05 μmol/L) or 6h (14.59 ± 4.38μmol/L) and iron reached its lowest level at 96 h (3.08 ± 1.18 μmol/L) or 16h (4.06 ± 1.58 μmol/L) after CFA administration or LPS infusion, respectively. This study demonstrated that the iron regulatory hormone hepcidin was up-regulated in sheep liver in response to systemic inflammation. These findings extend our knowledge on the relationship between the systemic inflammatory response, hepcidin and iron, and provide a starting point for additional studies on iron metabolism and the inflammatory process in sheep.
authors: Department of Veterinary Clinical Science, School of Veterinary Medicine and Animal Science, Univ Estadual Paulista (UNESP), Botucatu, SP, Brazil.
authors: Peres R
authors: PR
authors: Badial
authors: José P
authors: JP
authors: Oliveira-Filho
authors: Paulo Henrique J
authors: PH
authors: Cunha
authors: João P
authors: JP
authors: Araújo
authors: Juliana R
authors: JR
authors: Peiró
authors: Thomas J
authors: TJ
authors: Divers
authors: Nena J
authors: NJ
authors: Winand
authors: Alexandre S
authors: AS
authors: Borges
mesh_terms: D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000096:biosynthesis / Q000235:genetics*; D005340:Fibrinogen / Q000032:analysis; D020869:Gene Expression Profiling; D064451:Hepcidins; D007249:Inflammation / Q000235:genetics / Q000276:immunology / Q000662:veterinary*; D007501:Iron / Q000097:blood*; D008099:Liver / Q000378:metabolism; D008297:Male; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012756:Sheep / Q000097:blood / Q000235:genetics* / Q000276:immunology
chemical_list: D023181:Antimicrobial Cationic Peptides; D064451:Hepcidins; D005340:Fibrinogen; D007501:Iron