title: PAA-derived gold nanorods for cellular targeting and photothermal therapy.
abstract: A single-step LbL procedure to functionalize CTAB-capped GNRs via electrostatic self-assembly is reported. This approach allows for consistent biomolecule/GNR coupling using standard carboxyl-amine conjugation chemistry. The focus is on cancer-targeting biomolecule/GNR conjugates and selective photothermal destruction of cancer cells by GNR-mediated hyperthermia and NIR light. GNRs were conjugated to a single-chain antibody selective for colorectal carcinoma cells and used as probes to demonstrate photothermal therapy. Selective targeting and GNR uptake in antigen-expressing SW 1222 cells were observed using fluorescence microscopy. Selective photothermal therapy is demonstrated using SW 1222 cells, where >62% cell death was observed after cells are treated with targeted A33scFv-GNRs.
authors: Graduate Field of Biomedical Engineering, Cornell University, Ithaca, NY 14853, USA. dk322@cornell.edu
authors: Dickson K
authors: DK
authors: Kirui
authors: Sushmitha
authors: S
authors: Krishnan
authors: Aaron D
authors: AD
authors: Strickland
authors: Carl A
authors: CA
authors: Batt
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry; D015179:Colorectal Neoplasms / Q000188:drug therapy* / Q000473:pathology; D016650:Fluorescein-5-isothiocyanate / Q000032:analysis; D006046:Gold / Q000737:chemistry; D006358:Hot Temperature / Q000627:therapeutic use; D006801:Humans; D018796:Immunoconjugates / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D007259:Infrared Rays / Q000627:therapeutic use; D058990:Molecular Targeted Therapy / Q000379:methods*; D043942:Nanotubes / Q000737:chemistry; D010316:Particle Size; D010789:Phototherapy / Q000295:instrumentation / Q000379:methods*; D057127:Single-Chain Antibodies / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D013501:Surface-Active Agents / Q000737:chemistry
chemical_list: D000180:Acrylic Resins; D002593:Cetrimonium Compounds; D018796:Immunoconjugates; D057127:Single-Chain Antibodies; D013501:Surface-Active Agents; C006903:carbopol 940; D006046:Gold; D016650:Fluorescein-5-isothiocyanate; D000077286:Cetrimonium