title: Genetic and expressional alterations of CHD genes in gastric and colorectal cancers.
abstract: OBJECTIVE
  Chromodomain helicase DNA-binding protein (CHD) is a regulator of the chromatin remodelling process. The aim was to determine the CHD1, CHD2, CHD3, CHD4, CHD7, CHD8 and CHD9 mutational status of mononucleotide repeats in gastric and colorectal cancers with microsatellite instability (MSI).
  
  
  RESULTS
  The repeats were determined in 28 gastric cancers (GCs) with high MSI (MSI-H), 45 GCs with low MSI (MSI-L)/stable MSI (MSS), 35 colorectal cancers (CRCs) with MSI-H and 45 CRCs with MSI-L/MSS by single-strand conformation polymorphism analysis. CHD4 and CHD8 expression was also examined in GCs and CRCs by immunohistochemistry. CHD1, CHD2, CHD3, CHD4, CHD7, CHD8 and CHD9 mutations were found in five, 19, three, five, seven, 10 and seven cancers, respectively. They were detected in MSI-H cancers, but not in MSI-L/MSS cancers. Loss of CHD4 expression was observed in 56.4% of the GCs and 55.7% of the CRCs, and loss of CHD8 was observed in 35.7% of the GCs and 28.6% of the CRCs. The cancers with CHD4 and CHD8 mutations showed loss of CHD4 and CHD8 expression, respectively.
  
  
  CONCLUSIONS
  Frameshift mutation and loss of expression of CHD genes are common in GCs and CRCs with MSI-H.These alterations might contribute to cancer pathogenesis by deregulating CHD-mediated chromatin remodelling.
authors: Department of Pathology, College of Medicine, The Catholic University of Korea, Seoul, Korea.
authors: Min Sung
authors: MS
authors: Kim
authors: Nak Gyun
authors: NG
authors: Chung
authors: Mi Ran
authors: MR
authors: Kang
authors: Nam Jin
authors: NJ
authors: Yoo
authors: Sug Hyung
authors: SH
authors: Lee
mesh_terms: D015179:Colorectal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D004265:DNA Helicases / Q000235:genetics* / Q000378:metabolism*; D004273:DNA, Neoplasm / Q000378:metabolism; D016368:Frameshift Mutation; D015972:Gene Expression Regulation, Neoplastic; D053842:Microsatellite Instability; D018895:Microsatellite Repeats; D018807:Polymorphism, Single-Stranded Conformational; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology
chemical_list: D004273:DNA, Neoplasm; D004265:DNA Helicases