title: Tenacibaculum dicentrarchi sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from European sea bass.
abstract: A novel Gram-stain-negative rod-shaped gliding bacterial strain, designated 35/09(T), was isolated from diseased European sea bass (Dicentrarchus labrax L.) in Spain. Colonies were pale-yellow-pigmented with uneven edges and did not adhere to the agar. The DNA G+C content of the isolate was 31.3 mol%. 16S rRNA gene sequence analysis indicated affiliation to the genus Tenacibaculum (family Flavobacteriaceae, phylum 'Bacteroidetes'). Sequence similarities between the isolate and type strains of other members of the genus were 93.1-97.3 %. The major fatty acids (>5 % of the total fatty acids) were iso-C(15 : 0) (24.8 %), iso-C(15 : 0) 3-OH (18.0 %), anteiso-C(15 : 0) (8.1 %), C(15 : 1)ω6c (6.9 %) and iso-C(15 : 1) (6.2 %). Genotypic and phenotypic data indicate that strain 35/09(T) should be classified as a representative of a novel species in the genus Tenacibaculum, for which the name Tenacibaculum dicentrarchi sp. nov. is proposed; the type strain is 35/09(T) ( = CECT 7612(T) = NCIMB 14598(T)).
authors: Departamento de Microbiología y Parasitología, Centro de Investigaciones Biológicas (CIBUS), Universidad de Santiago de Compostela, Campus Sur 15782, Santiago de Compostela, Spain.
authors: Maximino
authors: M
authors: Piñeiro-Vidal
authors: Skretting, Ctra. de la Estación, Cojobar, Burgos 09620, Spain.
authors: Daniel
authors: D
authors: Gijón
authors: Skretting, Ctra. de la Estación, Cojobar, Burgos 09620, Spain.
authors: Carles
authors: C
authors: Zarza
authors: Departamento de Microbiología y Parasitología, Centro de Investigaciones Biológicas (CIBUS), Universidad de Santiago de Compostela, Campus Sur 15782, Santiago de Compostela, Spain.
authors: Ysabel
authors: Y
authors: Santos
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D001492:Bass / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005393:Fish Diseases / Q000382:microbiology*; D045826:Flavobacteriaceae Infections / Q000382:microbiology / Q000662:veterinary*; D020459:Genes, rRNA; D005838:Genotype; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013030:Spain; D060493:Tenacibaculum / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S