title: [Determination of aconitine and hypaconitine in Gucixiaotong Ye by capillary electrophoresis with field-amplified sample injection].
abstract: OBJECTIVE
  To set up a capillary electrophoresis method with field-amplified sample injection for the determination f aconitine and hypaconitine in Gucixiaotong Ye.
  
  
  METHODS
  An uncoated fused-silica capillary column (50 microm x 50 cm, effective length 42 cm) was used as the separation channel. The running buffer was 50 mmol x L(-1) phosphate electrolyte solution (pH 9)-m nol (90:10) , the running voltage was 10 kV and the capillary inlet was dipped in methanol for 5 s prior to electrokinetic injection (12 kV, 30 s), the detection wavelength was set at 235 nm.
  
  
  RESULTS
  Aconitine and hypaconitine were linear in the concentration ranges of 17.2-275 microg x L(-1) and 34. 4-550 microg x L(-1), respectively. The average recovery was more than 93.9% with the RSD of 3.8%. This method could enrich 500 fold of aconitine alkaloid.
  
  
  CONCLUSIONS
  The method is simple, rapid and specific with high stacking efficiency, it provides a new reliable means for production and quality control of Gucixiaotong Ye.
authors: Department of Pharmacy, Hebei Medical University, Shijiazhuang 050017, China. licencom@126.com
authors: Cen
authors: C
authors: Li
authors: Ye
authors: Y
authors: Jiang
mesh_terms: D000157:Aconitine / Q000031:analogs & derivatives* / Q000032:analysis* / Q000302:isolation & purification; D002021:Buffers; D004560:Electricity; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D007267:Injections; D012996:Solutions; D013997:Time Factors
chemical_list: D002021:Buffers; D012996:Solutions; C058309:hypaconitine; D000157:Aconitine