title: Causal involvement of mammalian-type cryptochrome in the circadian cuticle deposition rhythm in the bean bug Riptortus pedestris.
abstract: Mammalian-type CRYPTOCHROME (CRY-m) is considered to be a core repressive component of the circadian clock in various insect species. However, this role is based only on the molecular function of CRY-m in cultured cells and it therefore remains unknown whether CRY-m is indispensable for governing physiological rhythms at the organismal level. In the present study, we show that RNA interference (RNAi) targeting of cry-m in the bean bug Riptortus pedestris disrupts the circadian clock governing the cuticle deposition rhythm and results in the generation of a single cuticle layer. Furthermore, period expression was induced in cry-m RNAi insects. These results verified that CRY-m functions as a negative regulator in the circadian clock that generates physiological rhythm at the organismal level.
authors: Graduate School of Science, Osaka City University, Osaka, Japan Institute of Low Temperature Science, Hokkaido University, Sapporo, Japan Graduate School of Science, Kyoto University, Kyoto, Japan.
authors: T
authors: T
authors: Ikeno
authors: C
authors: C
authors: Katagiri
authors: H
authors: H
authors: Numata
authors: S G
authors: SG
authors: Goto
mesh_terms: D000818:Animals; D057906:Circadian Clocks; D002940:Circadian Rhythm; D056931:Cryptochromes / Q000235:genetics / Q000502:physiology*; D020868:Gene Silencing; D020062:Heteroptera / Q000235:genetics / Q000502:physiology*; D019476:Insect Proteins / Q000502:physiology; D056950:Period Circadian Proteins / Q000235:genetics / Q000502:physiology*; D034622:RNA Interference
chemical_list: D056931:Cryptochromes; D019476:Insect Proteins; D056950:Period Circadian Proteins; C047663:cuticle proteins, insects