title: Study of biochemical pathway and enzyme involved in metsulfuron-methyl degradation by Ancylobacter sp. XJ-412-1 isolated from soil.
abstract: Ancylobacter sp. XJ-412-1, capable of degrading metsulfuron-methyl, was isolated from sulfonylurea-contaminated soil. When metsulfuron-methyl was provided as the sole carbon source, more than 90.5% of metsulfuron-methyl at concentration of 50 mg l(-1) was degraded by strain XJ-412-1 after incubation at 30°C for 7 days. The initial degradation products of metsulfuron-methyl (MSM), thifensulfuron-methyl (TSM), and bensulfuron-methyl (BSM) by XJ-412-1 were identified as corresponding deesterified derivatives by liquid chromatography-mass spectrometry, which indicated a primary pathway of the deesterification of these three sulfonylurea herbicides. The carboxyesterase activity of the cell-free extracts was assayed and strongly inhibited by 4-chloromercuribenzoic acid (PCMB), diethyl pyrocarbonate (DEPC), phenylmethylsulfonyl fluoride (PMSF), and malathion.
authors: Department of Microbiology, Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, People's Republic of China.
authors: Peng
authors: P
authors: Lu
authors: Lei
authors: L
authors: Jin
authors: Bin
authors: B
authors: Liang
authors: Jing
authors: J
authors: Zhang
authors: Shunpeng
authors: S
authors: Li
authors: Zhaozhong
authors: Z
authors: Feng
authors: Xing
authors: X
authors: Huang
mesh_terms: D020561:Alphaproteobacteria / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D001190:Arylsulfonates / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D043182:Carboxylesterase / Q000235:genetics / Q000378:metabolism*; D006540:Herbicides / Q000378:metabolism*; D008969:Molecular Sequence Data; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism
chemical_list: D001190:Arylsulfonates; D001426:Bacterial Proteins; D006540:Herbicides; D012989:Soil Pollutants; C050296:metsulfuron methyl; D043182:Carboxylesterase