title: Adipose-derived stem cells promote gastric cancer cell growth, migration and invasion through SDF-1/CXCR4 axis.
abstract: OBJECTIVE
  The CXCR4/SDF-1 axis regulates the trafficking of various cell types. In the study we investigated whether adipose-derived stem cells can promote gastric cancer cell growth and migration through SDF-1/CXCR4 axis.
  
  
  METHODS
  The expression of CXCR4 in gastric cancer cell line MGC-803 and SDF-1 in Adipose-derived stem cells was detected by using PCR method. Furthermore, cell proliferation, migration and invasion assays were examined and inhibitors of CXCR4 were used to determine the effect on cell migration.
  
  
  RESULTS
  The cells isolated from subcutaneous adipose tissue show characteristics of stem cells. Gastric cancer cells express CXCR4 and Adipose-derived stem cells express SDF-1. Additionally, the tumor cells showed vigorous proliferative and migratory responses to ADSCs. AMD3100 can effectively reduce the migration of gastric cancer cells.
  
  
  CONCLUSIONS
  Adipose-derived stem cells can promote gastric cancer cell growth, migration and invasion through SDF-1/CXCR4 axis. CXCR4 can be used in the treatment of gastric cancer as a potential therapeutic target.
authors: Department of General Surgery, Beijing Chaoyang Hospital, Capital Medical University, No. 8 Gongtinan Lu, Chaoyang District, Beijing 100020, PR China.
authors: Bao-Cheng
authors: BC
authors: Zhao
authors: Bo
authors: B
authors: Zhao
authors: Jia-Gang
authors: JG
authors: Han
authors: Hua-Chong
authors: HC
authors: Ma
authors: Zhen-Jun
authors: ZJ
authors: Wang
mesh_terms: D017667:Adipocytes / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D054377:Chemokine CXCL12 / Q000378:metabolism*; D018920:Coculture Techniques; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D006801:Humans; D009361:Neoplasm Invasiveness / Q000473:pathology; D010641:Phenotype; D016133:Polymerase Chain Reaction; D019718:Receptors, CXCR4 / Q000378:metabolism*; D013234:Stem Cells / Q000473:pathology*; D013274:Stomach Neoplasms / Q000473:pathology*
chemical_list: C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; D019718:Receptors, CXCR4