title: Prevalence of factor V Leiden in south Tunisian blood donors.
abstract: Venous thrombosis (VT) is a common disease with multifactorial pathogenesis. Factor V Leiden mutation (G1691A) (FVL) is the most common risk factor in venous thrombosis. The prevalence of FVL varies according to geography and ethnicity. Hence, in several countries there is a difference in the frequency of this mutation between the southern, central and north. In Tunisia, no data is available about prevalence of FVL mutation by geographical origin. For this reason, we sought the prevalence of FVL mutation in blood donor of south Tunisia population. FVL has been detected by APCR-test and confirmed by PCR-RFLP and sequencing. Two hundred fifty blood donors, different in age and sex were included in this study to determine the prevalence of FVL in blood donors. FVL mutation was found in 13.6% of the studied population. Thirty-one were heterozygous and three persons were homozygous with a rate of 12.4 and 1.2%, respectively. In conclusion, FVL mutation is very common in south Tunisian population.
authors: Faculté de Médecine de Sfax Centre Régionale de Transfusion Sanguine de Sfax, Université de Sfax-Tunisia, Route Majida Boulila, Tunisia.
authors: Lobna
authors: L
authors: Maalej
authors: Basma
authors: B
authors: Hadjkacem
authors: Ikram
authors: I
authors: Ben Amor
authors: Mariem
authors: M
authors: Smaoui
authors: Ali
authors: A
authors: Gargouri
authors: Jalel
authors: J
authors: Gargouri
mesh_terms: D019943:Amino Acid Substitution; D001782:Blood Donors; D005165:Factor V / Q000235:genetics*; D005260:Female; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D008297:Male; D020125:Mutation, Missense; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D015995:Prevalence; D014416:Tunisia
chemical_list: C095381:factor V Leiden; D005165:Factor V