title: A suction-type microfluidic immunosensing chip for rapid detection of the dengue virus.
abstract: The enzyme-linked immunosorbent assay (ELISA) is widely used in medical diagnostics. In order to reduce the diagnosis time and to lower the consumption of sample/reagents in an ELISA assay, a suction-type, automatic, pneumatically-driven microfluidic chip has been designed and fabricated in this study. The microfluidic chip integrates a multi-functional micro-transport/mixing unit, for transporting metering and mixing of samples and reagents in order to automatically perform the entire ELISA protocol. A new surface modification has been adopted which allows for a high processing capacity. The detection sensitivity for the dengue virus is found to be 10(1) PFU/ml, which is much better than a conventional ELISA assay (10(3) PFU/ml). The entire assay time is only 30 min, which is much faster than with 96-well microtiter plates (4 h). The consumed sample and reagent volume is only 12 μl, which is less than a conventional assay (100 μl). The development of this microfluidic chip may be promising for other immunosensing applications.
authors: Department of Microbiology and Immunology, National Cheng Kung University, Tainan, Taiwan.
authors: Chen-Hsun
authors: CH
authors: Weng
authors: Tze-Bin
authors: TB
authors: Huang
authors: Chih-Chia
authors: CC
authors: Huang
authors: Chen-Sheng
authors: CS
authors: Yeh
authors: Huan-Yao
authors: HY
authors: Lei
authors: Gwo-Bin
authors: GB
authors: Lee
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D003716:Dengue Virus / Q000302:isolation & purification*; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D007118:Immunoassay / Q000295:instrumentation*; D057230:Limit of Detection; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D013997:Time Factors
chemical_list: D004129:Dimethylpolysiloxanes; C013830:baysilon