title: An HPLC method for determination of azadirachtin residues in bovine muscle.
abstract: A high-performance liquid chromatography (HPLC) method for the determination of azadirachtin (A and B) residues in bovine muscle has been developed. Azadirachtin is a neutral triterpene and chemotherapeutic agent effective in controlling some pest flies in horses, stables, horns and fruit. The actual HPLC method uses an isocratic elution and UV detection. Liquid-liquid extraction and solid-phase purification was used for the clean-up of the biological matrix. The chromatographic determination of these components is achieved using a C18 analytical column with water-acetonitrile mixture (27.5:72.5, v/v) as mobile phase, 1 mL/min as flow rate, 45 °C column temperature and UV detector at 215 nm. The azadirachtin peaks are well resolved and free of interference from matrix components. The extraction and analytical method developed in this work allows the quantitation of azadirachtin with precision and accuracy, establishing a lower limit of quantitation of azadirachtin, extracted from the biological matrix.
authors: Universidad de Chile, Facultad de Ciencias Químicas y Farmacéuticas, Departamento de Ciencias y Tecnología Farmacéuticas, Vicuña Mackenna 20, Casilla 233, Santiago, Chile. mgai@uchile.cl
authors: María Nella
authors: MN
authors: Gai
authors: Christian
authors: C
authors: Álvarez
authors: Raúl
authors: R
authors: Venegas
authors: Javier
authors: J
authors: Morales
mesh_terms: D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase; D036701:Limonins / Q000032:analysis*; D016014:Linear Models; D008460:Meat / Q000032:analysis*; D010573:Pesticide Residues / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
chemical_list: D036701:Limonins; D010573:Pesticide Residues; C010329:azadirachtin