title: Circadian transcriptional factor DBP regulates expression of Kiss1 in the anteroventral periventricular nucleus.
abstract: The expression of Kiss1 in the anteroventral periventricular nucleus (AVPV) and its product, metastin/kisspeptin, show a circadian pattern with a peak in the evening, which shows a strong phase relationship with the time of the gonadotropin-releasing hormone (GnRH)/luteinizing hormone (LH) surge in rodents. Here we report that a circadian transcriptional factor, albumin D-site binding protein (Dbp), was able to trigger mKiss1 transcription via the D-box, and this effect was combined with those of estrogen receptor α (ERα) and its ligand, estrogen. A histological study demonstrated that some cells in the AVPV co-expressed Dbp with ERα in adult female rats. Expression of ERα was not rhythmic in the AVPV, however, mRNA of Dbp in the AVPV accumulated with a robust diurnal rhythm in proestrus, but not on the first day of diestrus. Thus, these results suggest that Dbp and estrogen regulate the expression of Kiss1 in the AVPV, thereby mediating the GnRH/LH surge.
authors: Graduate School of Science and Engineering, Saitama University, Saitama 338-8570, Japan.
authors: Zhifang
authors: Z
authors: Xu
authors: Shigehito
authors: S
authors: Kaga
authors: Jun
authors: J
authors: Tsubomizu
authors: Jun
authors: J
authors: Fujisaki
authors: Akikazu
authors: A
authors: Mochiduki
authors: Takafumi
authors: T
authors: Sakai
authors: Hiroko
authors: H
authors: Tsukamura
authors: Kei-ichiro
authors: K
authors: Maeda
authors: Kinji
authors: K
authors: Inoue
authors: Akihito A
authors: AA
authors: Adachi
mesh_terms: D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D002940:Circadian Rhythm; D004268:DNA-Binding Proteins / Q000378:metabolism*; D047628:Estrogen Receptor alpha / Q000235:genetics / Q000378:metabolism; D004967:Estrogens / Q000378:metabolism; D030762:Estrous Cycle / Q000235:genetics; D005260:Female; D017930:Genes, Reporter; D059648:Kisspeptins; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D020644:Midline Thalamic Nuclei / Q000166:cytology / Q000378:metabolism*; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D020218:Response Elements; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic; D015854:Up-Regulation
chemical_list: C485975:DBP protein, rat; D004268:DNA-Binding Proteins; D047628:Estrogen Receptor alpha; D004967:Estrogens; C491406:Kiss1 protein, rat; D059648:Kisspeptins; D011506:Proteins; D014157:Transcription Factors; D008156:Luciferases