title: Potential antibiotic and anti-infective effects of rhodomyrtone from Rhodomyrtus tomentosa (Aiton) Hassk. on Streptococcus pyogenes as revealed by proteomics.
abstract: Rhodomyrtone from Rhodomyrtus tomentosa (Aiton) Hassk. leaf extract has a strong antibacterial activity against the bacterial pathogen Streptococcus pyogenes. Our previous studies indicated that the bactericidal activity of rhodomyrtone might involve intracellular targets. In the present studies we followed a proteomics approach to investigate the mode of action of rhodomyrtone on S. pyogenes. For this purpose, S. pyogenes was cultivated in the presence of 0.39 μg/ml rhodomyrtone, which corresponds to 50% of the minimal inhibitory concentration. The results show that the amounts of various enzymes associated with important metabolic pathways were strongly affected, which is consistent with the growth-inhibiting effect of rhodomyrtone. Additionally, cells of S. pyogenes grown in the presence of rhodomyrtone produced reduced amounts of known virulence factors, such as the glyceraldehyde-3-phosphate dehydrogenase, the CAMP factor, and the streptococcal pyrogenic exotoxin C. Taken together, these findings indicate that rhodomyrtone has both antimicrobial and anti-infective activities, which make it an interesting candidate drug.
authors: Faculty of Traditional Thai Medicine and Natural Products Research Center, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand.
authors: Surasak
authors: S
authors: Limsuwan
authors: Anne
authors: A
authors: Hesseling-Meinders
authors: Supayang Piyawan
authors: SP
authors: Voravuthikunchai
authors: Jan Maarten
authors: JM
authors: van Dijl
authors: Oliver
authors: O
authors: Kayser
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000890:Anti-Infective Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D053858:Metabolic Networks and Pathways; D008826:Microbial Sensitivity Tests; D027822:Myrtaceae / Q000737:chemistry*; D010449:Peptide Mapping; D010936:Plant Extracts / Q000494:pharmacology; D040901:Proteomics; D013297:Streptococcus pyogenes / Q000187:drug effects* / Q000254:growth & development; D037521:Virulence Factors / Q000378:metabolism; D044004:Xanthones / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D001426:Bacterial Proteins; D010936:Plant Extracts; D037521:Virulence Factors; D044004:Xanthones; C541823:rhodomyrtone; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases