title: Size-dependent self-assembly of submicron/nano beads-protein conjugates for construction of a protein nanoarray.
abstract: A protein nanoarray is created when submicro and nano beads, varying in their size and each conjugated with different proteins, self-assemble to specific locations depending on the diameter matching the surface electron beam patterns created. Protein binding is confirmed from the fluorescence attenuation of the beads upon antigen-antibody binding on the bead surface. This method, called size-dependent self-assembly, allows control of the location of each type of bead, and thus, control of the location of multiple proteins. It provides fast multi-component patterning with a high binding resolution, which can be detected using a fluorescent light microscope. This method is developed to be a simple stand-alone tool for analysis of protein interactions. In addition, it has the potential to be used in conjunction with other methods protein analysis methods, such as enzyme-linked immunosorbent assay (ELISA) and atomic force microscopy (AFM).
authors: Department of Agricultural and Biosystems Engineering, The University of Arizona, Tucson, AZ 85721-0038, USA.
authors: Tremaine B
authors: TB
authors: Powell
authors: Phat L
authors: PL
authors: Tran
authors: Keesung
authors: K
authors: Kim
authors: Jeong-Yeol
authors: JY
authors: Yoon