title: Acute lung injury and change in expression of aquaporins 1 and 5 in a rat model of acute pancreatitis.
abstract: OBJECTIVE
  We aimed to examine aquaporins 1 and 5 (AQP1 and 5) expression in lung tissues of rats with early acute lung injury caused by severe acute pancreatitis (SAP) and roles of AQP1 and 5 in pulmonary edema development.
  
  
  METHODS
  Eighty Sprague-Dawley rats were randomized into the SAP group and the sham operation group. SAP model was prepared through retrograde injection of 5% taurocholic acid. We examined SAP rats for any early lung tissue damage and for AQP1 and 5 expression in lung tissues at different time points (3, 6, 12, and 24 hours) during disease development.
  
  
  RESULTS
  Immunofluorescence staining revealed that AQP1 and 5 expression in the SAP lung tissues did no significantly change 3 hours post-experiment, but gradually decreased from 6 hours compared with controls. Real-time RT-PCR analysis showed that AQP1 mRNA levels of the SAP rats were significantly higher than those of controls 3 hours post-experiment (p = 0.001). AQP1 and 5 mRNA levels significantly decreased 6 hours post-experiment (p < 0.001). Western blotting analysis showed that AQP1 and 5 expression significantly decreased from 6 hours (p < 0.001).
  
  
  CONCLUSIONS
  Decreased lung AQP1 and 5 expression may be related to pulmonary edema de velopment and increased severity of lung injury and pulmonary edema, which provides an additional mechanism for pancreatitis-associated lung injury.
authors: Department of General surgery, Affiliated Union Hospital, Fujian Medical University, No. 29, Xinquan road, Fuzhou, Fujian 350001, China.
authors: Feng
authors: F
authors: Wang
authors: Heguang
authors: H
authors: Huang
authors: Fengchun
authors: F
authors: Lu
authors: Yanchang
authors: Y
authors: Chen
mesh_terms: D055371:Acute Lung Injury / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D000704:Analysis of Variance; D000818:Animals; D051398:Aquaporin 1 / Q000378:metabolism; D051402:Aquaporin 5 / Q000378:metabolism; D015153:Blotting, Western; D005455:Fluorescent Antibody Technique; D010195:Pancreatitis / Q000150:complications / Q000378:metabolism / Q000473:pathology; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D051402:Aquaporin 5; D051398:Aquaporin 1