title: Expression of tankyrase 1 in gastric cancer and its correlation with telomerase activity.
abstract: Tankyrase 1, which enhances telomerase access to telomeres, plays an important role in telomere maintenance. The aim of this study was to determine the expression and potential role of tankyrase 1 in gastric cancer development and progression. We examined the expression of tankyrase 1 by RT-PCR and Western blotting, and assessed telomerase activity by TRAP-ELISA method in gastric cancer and adjacent normal tissues. We found that tankyrase 1 expression was significantly up-regulated in gastric cancer tissues compared to normal corresponding tissues. Tankyrase 1 over-expression by gastric cancerous tissue was significantly associated with tumor histology differentiation and tumor stage. Moreover, tankyrase 1 expression was significantly correlation with telomerase activity. Our results indicate that tankyrase 1 over-expression may play an important role in gastric cancer development and progression. Tankyrase 1 may be used as a biomarker of gastric cancer and may serve as a target for cancer therapy.
authors: Department of Surgery, Huazhong University of Science and Technology, Wuhan, Hubei, China. drgaojinbo@gmail.com
authors: Jinbo
authors: J
authors: Gao
authors: Jinghui
authors: J
authors: Zhang
authors: Yaoping
authors: Y
authors: Long
authors: Yuan
authors: Y
authors: Tian
authors: Xiaoming
authors: X
authors: Lu
mesh_terms: D000328:Adult; D000368:Aged; D015153:Blotting, Western; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D038501:Tankyrases / Q000235:genetics* / Q000378:metabolism; D019098:Telomerase / Q000235:genetics* / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D038501:Tankyrases; D019098:Telomerase; C418109:TNKS protein, human; C509186:TERT protein, human