title: Quantitative detection of adenosine in urine using silver enhancement of aptamer-gold nanoparticle aggregation and progressive dilution.
abstract: We implement the progressive dilution strategy to bring assays based on gold nanoparticles to a quantitative level. This is demonstrated by the detection of adenosine in urine by combining progressive dilution with the silver enhancement of aptamer-gold nanoparticle aggregation, giving good accuracy, high selectivity, and an unlimited dynamic range above LOD.
authors: Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
authors: Zhao Fang
authors: ZF
authors: Liu
authors: Jing
authors: J
authors: Ge
authors: Xin Sheng
authors: XS
authors: Zhao
mesh_terms: D000241:Adenosine / Q000652:urine*; D052157:Aptamers, Nucleotide / Q000138:chemical synthesis / Q000378:metabolism*; D015374:Biosensing Techniques; D006046:Gold / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007201:Indicator Dilution Techniques; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry; D012680:Sensitivity and Specificity; D012834:Silver / Q000737:chemistry / Q000378:metabolism
chemical_list: D052157:Aptamers, Nucleotide; D012834:Silver; D006046:Gold; D000241:Adenosine