title: Detection of single-base mutation in RNA using T4 RNA ligase-based nick-joining or DNAzyme-based nick-generation.
abstract: A single nucleotide polymorphism (SNP) is a common genetic variation when a single nucleotide differs between members of a species or paired chromosome. Due to its association with disease susceptibility and drug resistance, SNP detection is of great value in studying the variation in drug responses. Here we present two quantitative SNP detection methods for a single-base mismatch in RNA, based on nick-joining and nick-generating activities of T4 RNA ligase and DNAzyme, respectively. T4 RNA ligase successfully discriminated a one-base mismatch in the ligation junction, and the designed DNAzyme cleaved RNA by discerning a single-base mismatch in the cleaving site.
authors: Department of Bioscience and Biotechnology, and WCU and BRL Program, Konkuk University, Seoul, Republic of Korea.
authors: Kkothanahreum
authors: K
authors: Park
authors: Bo-Ra
authors: BR
authors: Choi
authors: Yea Seul
authors: YS
authors: Kim
authors: Seonmi
authors: S
authors: Shin
authors: Sang Soo
authors: SS
authors: Hah
authors: Woong
authors: W
authors: Jung
authors: Sangtaek
authors: S
authors: Oh
authors: Dong-Eun
authors: DE
authors: Kim
mesh_terms: D020137:Base Pair Mismatch; D021881:DNA, Catalytic / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel / Q000379:methods; D015336:Molecular Probe Techniques; D015345:Oligonucleotide Probes / Q000737:chemistry; D020641:Polymorphism, Single Nucleotide; D012313:RNA / Q000737:chemistry*; D011130:RNA Ligase (ATP) / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D021881:DNA, Catalytic; D015345:Oligonucleotide Probes; D014764:Viral Proteins; D012313:RNA; D011130:RNA Ligase (ATP); C467724:bacteriophage T4 RNA ligase 2