title: Association of stromal cell-derived factor-1-3'A polymorphism to higher mobilization of hematopoietic stem cells CD34+ in Tunisian population.
abstract: We explored the influence of polymorphisms in genes encoding the chemokine stromal cell-derived factor-1 (SDF-1)/CXCL12 in a cohort of Tunisian patients with malignant hematologic diseases multiple myeloma [MM], non-Hodgkin's lymphoma [NHL], Hodgkin's disease, and acute myeloid leukemia [AML], who underwent stem cell mobilization for autologous transplantation versus a group of healthy donors for allogeneic transplantation. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLp) analysis was used for rapid identification of genotypes. Significant associations for SDF1-3'A polymorphism were observed exclusively in patients with MM and NHL. While there was a lack of all association of SDF-1 polymorphism with AML patients. However, considering that the ability of mobilization varies among subjects, we have observed that the SDF1-3'A allele was associated with good mobilization capacity. Interestingly, the association was mainly observed among healthy allogeneic transplant donors where the analysis was not biased by background disease or chemotherapy (P=.010; odds ratio=2.603; confidence interval [95%]=1.239-5.466).
authors: Cellular Immunology and Cytometry and Cellular Therapy Laboratory, Apheris Unit, National Blood Transfusion Center, Tunis, Tunisia.
authors: M
authors: M
authors: Ben Nasr
authors: Z
authors: Z
authors: Reguaya
authors: L
authors: L
authors: Berraies
authors: M
authors: M
authors: Maamar
authors: S
authors: S
authors: Ladeb
authors: T
authors: T
authors: Ben Othmen
authors: F
authors: F
authors: Mellouli
authors: M
authors: M
authors: Béjaoui
authors: J
authors: J
authors: Domenech
authors: F
authors: F
authors: Jenhani
mesh_terms: D000483:Alleles; D018952:Antigens, CD34 / Q000096:biosynthesis*; D054377:Chemokine CXCL12 / Q000235:genetics*; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods*; D006689:Hodgkin Disease / Q000235:genetics; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics; D009101:Multiple Myeloma / Q000235:genetics; D016017:Odds Ratio; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D014184:Transplantation, Homologous; D014416:Tunisia
chemical_list: D018952:Antigens, CD34; D054377:Chemokine CXCL12