title: ISsaga is an ensemble of web-based methods for high throughput identification and semi-automatic annotation of insertion sequences in prokaryotic genomes.
abstract: Insertion sequences (ISs) play a key role in prokaryotic genome evolution but are seldom well annotated. We describe a web application pipeline, ISsaga (http://issaga.biotoul.fr/ISsaga/issaga_index.php), that provides computational tools and methods for high-quality IS annotation. It uses established ISfinder annotation standards and permits rapid processing of single or multiple prokaryote genomes. ISsaga provides general prediction and annotation tools, information on genome context of individual ISs and a graphical overview of IS distribution around the genome of interest.
authors: Laboratoire de Microbiologie et Génétique Moléculaires, CNRS 118, Route de Narbonne, 31062 Toulouse Cedex, France. alessandro.varani@ibcg.biotoul.fr
authors: Alessandro M
authors: AM
authors: Varani
authors: Patricia
authors: P
authors: Siguier
authors: Edith
authors: E
authors: Gourbeyre
authors: Vincent
authors: V
authors: Charneau
authors: Mick
authors: M
authors: Chandler
mesh_terms: D019295:Computational Biology; D004251:DNA Transposable Elements; D020745:Genome, Archaeal; D016680:Genome, Bacterial; D020407:Internet; D058977:Molecular Sequence Annotation / Q000379:methods*; D016366:Open Reading Frames; D015203:Reproducibility of Results; D012984:Software
chemical_list: D004251:DNA Transposable Elements