title: Chemical mimicry: hierarchical 1D TiO2@ZrO2 core-shell structures reminiscent of sponge spicules by the synergistic effect of silicatein-α and silintaphin-1.
abstract: In nature, mineralization of hard tissues occurs due to the synergistic effect of components present in the organic matrix of these tissues, with templating and catalytic effects. In Suberites domuncula, a well-studied example of the class of demosponges, silica formation is mediated and templated by an axial proteinaceous filament with silicatein-α, one of the main components. But so far, the effect of other organic constituents from the proteinaceous filament on the catalytic effect of silicatein-α has not been studied in detail. Here we describe the synthesis of core-shell TiO(2)@SiO(2) and TiO(2)@ZrO(2) nanofibers via grafting of silicatein-α onto a TiO(2) nanowire backbone followed by a coassembly of silintaphin-1 through its specifically interacting domains. We show for the first time a linker-free, one-step funtionalization of metal oxides with silicatein-α using glutamate tag. In the presence of silintaphin-1 silicatein-α facilitates the formation of a dense layer of SiO(2) or ZrO(2) on the TiO(2)@protein backbone template. The immobilization of silicatein-α onto TiO(2) probes was characterized by atomic force microscopy (AFM), optical light microscopy, and high-resolution transmission electron microscopy (HRTEM). The coassembly of silicatein-α and silintaphin-1 may contribute to biomimetic approaches that pursue a controlled formation of patterned biosilica-based biomaterials.
authors: Institut für Anorganische Chemie und Analytische Chemie, Johannes Gutenberg-Universität, Duesbergweg 10-14, D-55099 Mainz, Germany.
authors: Rute
authors: R
authors: André
authors: Muhammad Nawaz
authors: MN
authors: Tahir
authors: Thorben
authors: T
authors: Link
authors: Florian D
authors: FD
authors: Jochum
authors: Ute
authors: U
authors: Kolb
authors: Patrick
authors: P
authors: Theato
authors: Rüdiger
authors: R
authors: Berger
authors: Matthias
authors: M
authors: Wiens
authors: Heinz-Christoph
authors: HC
authors: Schröder
authors: Werner E G
authors: WE
authors: Müller
authors: Wolfgang
authors: W
authors: Tremel
mesh_terms: D000818:Animals; D040761:Biomimetic Materials / Q000737:chemistry*; D002403:Cathepsins / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry; D018698:Glutamic Acid / Q000737:chemistry; D053770:Nanowires / Q000737:chemistry; D047233:Suberites / Q000737:chemistry*; D014025:Titanium / Q000737:chemistry*; D015040:Zirconium / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; C112571:silicatein alpha; C009495:titanium dioxide; D018698:Glutamic Acid; D015040:Zirconium; D014025:Titanium; D002403:Cathepsins; C028541:zirconium oxide