title: Adjuvant arthritis influences expression of housekeeping genes.
abstract: OBJECTIVE
  We determined whether adjuvant arthritis (AA) affects expression of three housekeeping (HK) genes, glyceraldehyde-3-phosphate-dehydrogenase (GAPDH), β-actin and 18s ribosomal RNA (18s rRNA) in organs of Sprague-Dawley rats using the real-time reverse-transcription polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  Expression of 18s rRNA was unaffected by AA whereas that of β-actin was reduced by 52, 62 and 81% in the liver, kidney and intestine, respectively, but not in the heart. AA significantly reduced GAPDH expression in the intestine.
  
  
  CONCLUSIONS
  For reliable results, the HK gene must be carefully selected.
authors: Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, AB, T6G 2N8, Canada.
authors: Sherif
authors: S
authors: Hanafy
authors: Fakhreddin
authors: F
authors: Jamali
mesh_terms: D000199:Actins / Q000235:genetics*; D000818:Animals; D001169:Arthritis, Experimental / Q000235:genetics*; D015870:Gene Expression; D020043:Genes, Essential; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics*; D007413:Intestinal Mucosa / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000378:metabolism; D012337:RNA, Ribosomal, 18S / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000199:Actins; D012337:RNA, Ribosomal, 18S; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases