title: Combinatory effects of PBDEs and 17β-estradiol on MCF-7 cell proliferation and apoptosis.
abstract: In the present work, we analyzed whether polybrominated diphenyl ethers (PBDEs) (47, 99, 100 and 209) interfere with the effect of 17β-estradiol on the proliferation and apoptosis of the MCF-7 cell line. MCF-7 cells were cultured in DMEM without phenol red; upplemented with 5% charcoal-treated fetal bovine serum for 3 days with 10 nM 17β-estradiol; with 0.1 μM, 0.5 μM or 1 μM of the tested PBDE congeners; or with both 17β-estradiol and a congener. Cell proliferation was determined by measuring BrdU incorporation, and cell apoptosis was measured by caspase-9 activity. No PBDE congener had an effect on basal cell proliferation, but they all significantly decreased basal caspase-9 activity. An additive anti-apoptotic activity and ability to induce cell proliferation was observed in the presence of 17β-estradiol.
authors: Department of Physiology and Toxicology of Reproduction, Chair of Animal Physiology, Institute of Zoology, Jagiellonian University, Ingardena 6, PL 30-060 Kraków, Poland.
authors: Patrycja
authors: P
authors: Kwiecińska
authors: Anna
authors: A
authors: Wróbel
authors: Ewa Ł
authors: EŁ
authors: Gregoraszczuk
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D001943:Breast Neoplasms / Q000473:pathology; D053453:Caspase 9 / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D055768:Halogenated Diphenyl Ethers / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans
chemical_list: D003470:Culture Media; D055768:Halogenated Diphenyl Ethers; D004958:Estradiol; D053453:Caspase 9