title: A novel DNA nuclease is stimulated by association with the GINS complex.
abstract: Chromosomal DNA replication requires the spatial and temporal coordination of the activities of several complexes that constitute the replisome. A previously uncharacterized protein, encoded by TK1252 in the archaeon Thermococcus kodakaraensis, was shown to stably interact with the archaeal GINS complex in vivo, a central component of the archaeal replisome. Here, we document that this protein (TK1252p) is a processive, single-strand DNA-specific exonuclease that degrades DNA in the 5' → 3' direction. TK1252p binds specifically to the GINS15 subunit of T. kodakaraensis GINS complex and this interaction stimulates the exonuclease activity in vitro. This novel archaeal nuclease, designated GINS-associated nuclease (GAN), also forms a complex in vivo with the euryarchaeal-specific DNA polymerase D. Roles for GAN in replisome assembly and DNA replication are discussed.
authors: Institute for Bioscience and Biotechnology Research, 9600 Gudelsky Drive, Rockville, MD 20850, USA.
authors: Zhuo
authors: Z
authors: Li
authors: Miao
authors: M
authors: Pan
authors: Thomas J
authors: TJ
authors: Santangelo
authors: Wiebke
authors: W
authors: Chemnitz
authors: Wei
authors: W
authors: Yuan
authors: James L
authors: JL
authors: Edwards
authors: Jerard
authors: J
authors: Hurwitz
authors: John N
authors: JN
authors: Reeve
authors: Zvi
authors: Z
authors: Kelman
mesh_terms: D019843:Archaeal Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone / Q000302:isolation & purification / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D005090:Exodeoxyribonucleases / Q000302:isolation & purification / Q000378:metabolism*; D019712:Thermococcus / Q000201:enzymology
chemical_list: D019843:Archaeal Proteins; D002868:Chromosomal Proteins, Non-Histone; D004277:DNA, Single-Stranded; D004259:DNA-Directed DNA Polymerase; D005090:Exodeoxyribonucleases