title: Separation of acetins by counter-current chromatography.
abstract: In this work we report the purification of a crude acetin mixture into mono-, di- and triacetin by countercurrent chromatography. The process was initially tested on a small, semi-preparative scale (0.5 g) to determine its efficiency. The process was then scaled up to accommodate 2.5 g of crude reaction products containing a mixture of the acetins. The solvent system ethyl acetate/n-butanol/water 1:0.2:1 was used in all separation procedures. Mono-, di- and triacetins were separated similarly in the semi-preparative and preparative runs.
authors: Universidade Federal do Rio de Janeiro, Núcleo de Pesquisas de Produtos Naturais, Centro de Ciências da Saúde, Rio de Janeiro, RJ, Brazil.
authors: Bruno Amaral
authors: BA
authors: Meireles
authors: Shaft Corrêa
authors: SC
authors: Pinto
authors: Vera Lúcia Patrocinio
authors: VL
authors: Pereira
authors: Gilda Guimarães
authors: GG
authors: Leitão
mesh_terms: D000085:Acetates / Q000737:chemistry; D056804:Biofuels; D003377:Countercurrent Distribution / Q000379:methods*; D005989:Glycerides / Q000138:chemical synthesis / Q000302:isolation & purification*; D005990:Glycerol / Q000737:chemistry
chemical_list: D000085:Acetates; D056804:Biofuels; D005989:Glycerides; C007650:ethyl acetate; C038923:monoacetin; D005990:Glycerol