title: [Analysis and authentication of Fallopia multiflora and its adulterants by matK sequence].
abstract: OBJECTIVE
  To identify Fallopia multiflora from its adulterants by comparing their matK sequences.
  
  
  METHODS
  Genomic DNA of different materials was extracted using modified cetytrimethyl ammonium bromide (CTAB) method. The double-strand matK genes were amplified using PCR method and then sequenced. The data were analyzed in Clustral W and MEGA 4.0 software package.
  
  
  RESULTS
  Besides F. multiflora var. ciliinerve, the matK sequences of other adulterants show distinct differences with F. multiflora, whether for nucleotides substitutions or genetic distances; and the specific identifying sites for distinguishing F. multiflora and other Fallopia adulterants were found through further comparative analysis. Moreover, the 3 inspected materials were successfully authenticated by comparing the matK sequences.
  
  
  CONCLUSIONS
  matK sequences can be used for the molecular identification between F. multiflora and its adulterant species.
authors: Department of Pharmacy, General Hospital of Guangzhou Military Command, Guangzhou 510010, China. shengshujing@foxmail.com
authors: Shu-Jing
authors: SJ
authors: Sheng
authors: Ping
authors: P
authors: Yan
authors: Chuan-Jin
authors: CJ
authors: Zheng
authors: Shu-Jin
authors: SJ
authors: Zhao
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D018742:DNA, Chloroplast / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D004340:Drug Contamination; D004722:Endoribonucleases / Q000235:genetics*; D017343:Genes, Plant; D009713:Nucleotidyltransferases / Q000235:genetics*; D010598:Pharmacognosy; D010802:Phylogeny; D018517:Plant Roots / Q000235:genetics; D010946:Plants, Medicinal / Q000235:genetics*; D019664:Polygonaceae / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D018742:DNA, Chloroplast; D018744:DNA, Plant; D009713:Nucleotidyltransferases; C034038:mRNA maturase; D004722:Endoribonucleases