title: Immunohistochemical evaluation of Pi class glutathione S-transferase expression in invasive breast carcinoma.
abstract: OBJECTIVE
  The aim of our work was to determine the expression of Pi class glutathione S-transferase (GSTP1) in 43 samples of invasive breast carcinoma and compare results versus normal breast cells.
  
  
  BACKGROUND
  Breast cancer is the commonest cancer in women. Despite advances in early detection and more efficacious adjuvant chemotherapy, a part of patients with early-stage have reccurent disease. In these cases the development of resistance to therapy is observed. The glutathione S-transferases (GSTs) are potentially involved in tumour chemoresistance.
  
  
  METHODS
  Enzyme immunohistochemical method was chosen for the detection of GSTP1 and its expression was compared in breast cancer cells versus normal breast cells.
  
  
  RESULTS
  We have found that majority (63%) of breast carcinomas shows GSTP1 positivity (nuclear and cytoplasmic immunoreactivity). It is expected that GSTP1 positive tumours would show a poorer prognosis than GSTP1 negative ones.
  
  
  CONCLUSIONS
  Immunohistochemistry is a useful method for investigating the expression and cellular localization of GSTP1 within tumours. It may be applied to a routine clinical test and it can serve as a marker for resistance to chemotherapy (Tab. 1, Fig. 4, Ref. 20). Full Text in free PDF www.bmj.sk.
authors: Department of Anatomy, P.J. Safarik University, Faculty of Medicine, Kosice, Slovakia. janka.vecanova@upjs.sk
authors: J
authors: J
authors: Vecanova
authors: I
authors: I
authors: Hodorova
authors: J
authors: J
authors: Mihalik
authors: M
authors: M
authors: Benicky
authors: D
authors: D
authors: Kluchova
authors: S
authors: S
authors: Rybarova
mesh_terms: D001943:Breast Neoplasms / Q000737:chemistry*; D018270:Carcinoma, Ductal, Breast / Q000737:chemistry*; D018275:Carcinoma, Lobular / Q000737:chemistry*; D005260:Female; D051549:Glutathione S-Transferase pi / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry
chemical_list: D051549:Glutathione S-Transferase pi