title: The expression of GM-CSF and osteopontin in immunocompetent cells precedes the odontoblast differentiation following allogenic tooth transplantation in mice.
abstract: Dental pulp elaborates both bone and dentin under pathological conditions such as tooth replantation/transplantation. This study aims to clarify the expression of granulocyte macrophage colony-stimulating factor (GM-CSF) and osteopontin (OPN) in the process of reparative dentin formation by allogenic tooth transplantation using in situ hybridization for OPN and immunohistochemistry for GM-CSF and OPN at both levels of light and electron microscopes. Following the extraction of the mouse molar, the roots and pulp floor were resected and immediately allografted into the sublingual region. On days 1 to 3, immunocompetent cells such as macrophages and dendritic cells expressed both GM-CSF and OPN, and some of them were arranged along the pulp-dentin border and extended their cellular processes into the dentinal tubules. On days 5 to 7, tubular dentin formation commenced next to the preexisting dentin at the pulp horn where nestin-positive odontoblast-like cells were arranged. Until day 14, bone-like tissue formation occurred in the pulp chamber, where OPN-positive osteoblasts surrounded the bone matrix. These results suggest that the secretion of GM-CSF and OPN by immunocompetent cells such as macrophages and dendritic cells plays a role in the maturation of dendritic cells and the differentiation of odontoblasts, respectively, in the regenerated pulp tissue following tooth transplantation.
authors: Division of Anatomy and Cell Biology of the Hard Tissue, Department of Tissue Regeneration and Reconstruction, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
authors: Kotaro
authors: K
authors: Saito
authors: Mitsushiro
authors: M
authors: Nakatomi
authors: Hiroko
authors: H
authors: Ida-Yonemochi
authors: Shin-ichi
authors: S
authors: Kenmotsu
authors: Hayato
authors: H
authors: Ohshima
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism*; D003782:Dental Pulp / Q000166:cytology / Q000276:immunology; D003804:Dentin / Q000166:cytology / Q000378:metabolism; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis*; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008264:Macrophages / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008813:Mice, Inbred ICR; D008963:Molar / Q000166:cytology / Q000276:immunology / Q000637:transplantation*; D009060:Mouth Floor / Q000601:surgery; D009804:Odontoblasts / Q000166:cytology* / Q000378:metabolism; D053495:Osteopontin / Q000096:biosynthesis*; D014184:Transplantation, Homologous
chemical_list: D000949:Histocompatibility Antigens Class II; D053495:Osteopontin; D016178:Granulocyte-Macrophage Colony-Stimulating Factor