title: Estradiol worsens the syndrome of ischemia-reperfusion injury in an experimental lung transplantation model.
abstract: Ischemia-reperfusion injury (IRI) is a common complication after lung transplantation. There is evidence that reactive oxygen species are involved in its pathogenesis. We designed an experimental study to evaluate whether the administration of antioxidants to lung transplantation recipients protects against IRI and early acute rejection (AR). Twenty-five rats received left lung transplants after 6 h of ischemia. Fifty minutes before the reperfusion, groups of five rats received a single dose of desferrioxamine (20 mg/kg), estradiol (25 mg/kg), or melatonin (10 mg/kg). The animals were killed 48 h after surgery and the postoperative outcome, IRI, and AR were evaluated. The frequency of severe injury and of moderate-to-severe edema was higher in animals treated with estradiol than in the control group (P = 0.022 and P = 0.026, respectively). No significant changes in the degree of IRI or AR were observed in the groups treated with desferrioxamine or melatonin. In our study, treatment with the antioxidants melatonin or desferrioxamine before reperfusion had no effects on IRI damage or on AR frequency or severity. However, treatment with estradiol resulted in a worse postoperative outcome and in severe edema. Therefore, despite the antioxidant capacity of estradiol, it is recommended that an evaluation of these adverse effects of estradiol in human lung transplant recipients be performed.
authors: Experimental Surgery Unit, Research Unit, Hospital Universitario Dr. Negrín, Barranco de la Ballena s/n, 35010, Las Palmas de Gran Canaria, Spain. norbesr@terra.es
authors: Norberto
authors: N
authors: Santana-Rodríguez
authors: Bernardino
authors: B
authors: Clavo
authors: Pedro
authors: P
authors: Llontop
authors: Ana
authors: A
authors: López
authors: José Manuel
authors: JM
authors: García-Castellano
authors: Rubén P
authors: RP
authors: Machín
authors: Miguel A
authors: MA
authors: Ponce
authors: María D
authors: MD
authors: Fiuza
authors: Ricardo
authors: R
authors: García-Herrera
authors: Yanira
authors: Y
authors: Brito
authors: Nagib Atallah
authors: NA
authors: Yordi
authors: Ricardo
authors: R
authors: Chirino
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage; D016009:Chi-Square Distribution; D003676:Deferoxamine / Q000008:administration & dosage; D004195:Disease Models, Animal; D004958:Estradiol / Q000008:administration & dosage / Q000633:toxicity*; D006084:Graft Rejection / Q000209:etiology / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D006085:Graft Survival / Q000187:drug effects; D008168:Lung / Q000098:blood supply / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D055370:Lung Injury / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D016040:Lung Transplantation / Q000009:adverse effects*; D008297:Male; D008550:Melatonin / Q000008:administration & dosage; D018384:Oxidative Stress / Q000187:drug effects; D011654:Pulmonary Edema / Q000209:etiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D015427:Reperfusion Injury / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D012720:Severity of Illness Index; D013997:Time Factors
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D004958:Estradiol; D003676:Deferoxamine; D008550:Melatonin