title: Immunohistochemical analysis of human mesenchymal stem cells differentiating into chondrogenic, osteogenic, and adipogenic lineages.
abstract: Mesenchymal stem cells (MSCs) are multipotent cells that have the potential to differentiate into various mesenchymal lineages in vitro and in vivo. Due to their availability from tissues such as bone marrow, synovium, fat, and muscle, and their highly proliferative capacity, MSCs have evoked interest as a potential cell source for repair and regeneration of various types of tissues. Characterization by the expression of a panel of surface markers and the ability of MSCs to undergo multilineage differentiation is the benchmark for identifying this stem cell population. In this chapter, the protocols for the differentiation of MSC to chondrogenic, osteogenic, and adipogenic lineages and histological and immunostaining protocols for confirming trilineage differentiation of the MSC cells are described.
authors: Department of Orthopaedic Surgery, Yong Loo Lin School of Medicine, NUS Tissue Engineering Program National University of Singapore, Singapore, Singapore.
authors: Zheng
authors: Z
authors: Yang
authors: Jacqueline Frida
authors: JF
authors: Schmitt
authors: Eng Hin
authors: EH
authors: Lee
mesh_terms: D050156:Adipogenesis; D000423:Alcian Blue / Q000378:metabolism; D000818:Animals; D000880:Anthraquinones / Q000378:metabolism; D001391:Azo Compounds / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology; D018929:Cell Culture Techniques; D002469:Cell Separation; D020219:Chondrogenesis; D003094:Collagen / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D008903:Minerals / Q000378:metabolism; D010012:Osteogenesis; D010619:Phenazines / Q000378:metabolism; D014176:Protein Biosynthesis; D011509:Proteoglycans / Q000378:metabolism; D013194:Staining and Labeling
chemical_list: D000880:Anthraquinones; D001391:Azo Compounds; D008903:Minerals; D010619:Phenazines; D011509:Proteoglycans; C010078:alizarin; D003094:Collagen; C011049:oil red O; D000423:Alcian Blue; C009195:safranine T