title: Effects of an avidin-biotin binding system on Schwann cells attachment, proliferation, and gene expressions onto electrospun scaffolds.
abstract: Effective Schwann cells (SCs) attachment is a prerequisite for the successful construction of tissue-engineered nerve. The present study aimed to investigate the role of an avidin-biotin binding system (ABBS) for neural tissue engineering. The attachment, proliferation, and morphology of biotinylated SCs on avidin-treated scaffolds were examined, and the effects of avidin, biotin, and the avidin-biotin binding system on SCs gene expressions were also studied. The results indicated that the attachment of biotinylated SCs onto avidin-treated scaffolds was promoted obviously within a short time (10 min). Meanwhile, there were no great differences in terms of proliferation and morphology of SCs between the two groups after cultivation for 14 days. The gene expressions of S100, GDNF, BDNF, NGF, CNTF, and PMP22 were up-regulated significantly by biotin rather than aligned scaffolds or avidin. The present study demonstrated that ABBS enhanced the attachment and maturation of SCs onto the electrospun scaffolds without adverse effects on the proliferation of SCs in the long term, suggesting the potential application of ABBS in the neural tissue engineering.
authors: Department of Orthopedics, Zhongshan Hospital of Fudan University, 180 Fenglin Road, Shanghai 200032, People's Republic of China.
authors: Sha
authors: S
authors: Feng
authors: Zuoqin
authors: Z
authors: Yan
authors: Changan
authors: C
authors: Guo
authors: Zhengrong
authors: Z
authors: Chen
authors: Kuihua
authors: K
authors: Zhang
authors: Xiumei
authors: X
authors: Mo
authors: Yudong
authors: Y
authors: Gu
mesh_terms: D000818:Animals; D001360:Avidin / Q000378:metabolism*; D001710:Biotin / Q000378:metabolism*; D019921:Biotinylation; D002448:Cell Adhesion; D002460:Cell Line; D049109:Cell Proliferation; D015870:Gene Expression; D012583:Schwann Cells / Q000166:cytology* / Q000378:metabolism; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001360:Avidin; D001710:Biotin