title: Multiplex PCR for rapid diagnosis of tuberculous meningitis.
abstract: Rapid and specific diagnosis of tubercular meningitis is of paramount importance to decrease morbidity and mortality. The aim of the study was to evaluate multiplex PCR using protein b, MPB 64, and IS6110 primers directed against M. tuberculosis complex for the diagnosis of tuberculous meningitis (TBM). Multiplex PCR was performed on 18 TBM confirmed cases (culture was positive), 92 clinically suspected TBM cases and 100 non-TBM (control group) patients. Multiplex PCR had a sensitivity of 94.4% for confirmed cases and specificity of 100% for confirmed TBM cases. In 92 clinically diagnosed but unconfirmed TBM cases, multiplex PCR was positive in 84.78% cases. The overall sensitivity of microscopy, culture and multiplex cases were 1.81, 16.73, and 86.63% and specificity was 100, 100, and 100% respectively. Multiplex PCR using protein b, MPB 64, and IS6110 primers has a high sensitivity and specificity in diagnosis of tubercular meningitis.
authors: Department of Medical Microbiology, Post Graduate Institute of Medical Education and Research, Chandigarh, India. sharmakusum9@yahoo.co.in
authors: Sharma
authors: S
authors: Kusum
authors: Sharma
authors: S
authors: Aman
authors: Ray
authors: R
authors: Pallab
authors: Sharma Shiv
authors: SS
authors: Kumar
authors: Modi
authors: M
authors: Manish
authors: Prabhakar
authors: P
authors: Sudesh
authors: Varma
authors: V
authors: Subhash
authors: Sharma
authors: S
authors: Meera
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000134:cerebrospinal fluid; D006801:Humans; D007223:Infant; D008875:Middle Aged; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D014390:Tuberculosis, Meningeal / Q000134:cerebrospinal fluid* / Q000175:diagnosis*; D055815:Young Adult
chemical_list: D004269:DNA, Bacterial