title: Assessment of the microbiota of a mixed infection of the tongue using phenotypic and genotypic methods simultaneously and a review of the literature.
abstract: We assessed the microbiota of a tongue abscess in which twelve different aerobic and anaerobic bacteria were identified using fluorescent in situ hybridisation (FISH), sequencing of the 16S rRNA gene and phenotypic methods. By applying the 16S rRNA based probes directly on the clinical material, a quick insight of the bacteria present was obtained and the species which were not cultured but present in the abscess were identified.
authors: Department of Medical Microbiology, University Medical Center, Groningen, The Netherlands. a.c.m.veloo@med.umcg.nl
authors: A C M
authors: AC
authors: Veloo
authors: R H
authors: RH
authors: Schepers
authors: G W
authors: GW
authors: Welling
authors: J E
authors: JE
authors: Degener
mesh_terms: D000038:Abscess / Q000382:microbiology* / Q000473:pathology; D000293:Adolescent; D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D001424:Bacterial Infections / Q000382:microbiology* / Q000473:pathology; D015373:Bacterial Typing Techniques / Q000379:methods; D001431:Bacteriological Techniques / Q000379:methods*; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000379:methods; D014060:Tongue Diseases / Q000382:microbiology* / Q000473:pathology
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S