title: Photoelectrochemical competitive DNA hybridization assay using semiconductor quantum dot conjugated oligonucleotides.
abstract: A competitive DNA hybridization assay based on the photoelectrochemistry of the semiconductor quantum dot-single stranded DNA conjugates (QD-ssDNA) was developed. Hybridization of QD-ssDNA with the capture probe DNA immobilized on the indium-tin oxide electrodes enables photocurrent generation when the electrochemical cell was illuminated with a light source. Upon the competition between QD-ssDNA and single-stranded target DNA, the photocurrent response decreased with the increase in the target DNA concentration. A linear relationship between the photocurrent and the target DNA concentration was obtained (R(2) = 0.991). The selectivity of system towards the target DNA was also demonstrated using non-complementary sample.
authors: Department of Food Engineering, Hacettepe University, Beytepe, Ankara, Turkey.
authors: Deniz
authors: D
authors: Baş
authors: Ismail Hakki
authors: IH
authors: Boyaci
mesh_terms: D004247:DNA / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000737:chemistry; D055664:Electrochemical Techniques / Q000379:methods*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D009841:Oligonucleotides / Q000737:chemistry*; D010777:Photochemistry; D045663:Quantum Dots; D012666:Semiconductors; D012680:Sensitivity and Specificity
chemical_list: D004277:DNA, Single-Stranded; D009841:Oligonucleotides; D004247:DNA