title: Pivalic acid acts as a starter unit in a fatty acid and antibiotic biosynthetic pathway in Alicyclobacillus, Rhodococcus and Streptomyces.
abstract: A biosynthetic pathway using pivalic acid as a starter unit was found in three bacterial species, Alicyclobacillus acidoterrestris, Rhodococcus erythropolis and Streptomyces avermitilis. When deuterium-labelled pivalic acid was added to A. acidoterrestris and R. erythropolis nutrient media it was incorporated into fatty acids to give rise to tert-butyl fatty acids (t-FAs). In addition, in R. erythropolis, pivalic acid was transformed into two starter units, i.e. isobutyric and 2-methylbutyric acid, which served as precursors of corresponding iso-even FAs and anteiso-FAs. In S. avermitilis the biosynthesis also yielded all three branched FAs; apart from this pathway, both pivalic and 2-methylbutyric acids were incorporated into the antibiotic avermectin.
authors: Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20 Prague, Czech Republic. rezanka@biomed.cas.cz
authors: Tomáš
authors: T
authors: Rezanka
authors: Lucie
authors: L
authors: Siristova
authors: Olga
authors: O
authors: Schreiberová
authors: Michal
authors: M
authors: Rezanka
authors: Jan
authors: J
authors: Masák
authors: Karel
authors: K
authors: Melzoch
authors: Karel
authors: K
authors: Sigler
mesh_terms: D056450:Alicyclobacillus / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D002087:Butyrates / Q000378:metabolism; D005227:Fatty Acids / Q000096:biosynthesis*; D007559:Ivermectin / Q000031:analogs & derivatives / Q000378:metabolism; D010421:Pentanoic Acids / Q000378:metabolism*; D012240:Rhodococcus / Q000378:metabolism*; D013302:Streptomyces / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D002087:Butyrates; D005227:Fatty Acids; D010421:Pentanoic Acids; D007559:Ivermectin; C019264:avermectin; C005566:pivalic acid; C019475:2-methylbutanoic acid