title: Small interfering RNAs inhibit infectious bursal disease virus replication in Vero cells.
abstract: Small interfering RNA (siRNA) molecules are considered to be a promising antiviral therapeutics. This study was performed to analyze the application of siRNA against infectious bursal disease virus (IBDV) replication. Two siRNAs were designed to target common coding sequences of four IBDV proteins. Corresponding vectors were constructed to express anti-IBDV short hairpin RNAs (shRNA) that were tested for their antiviral effect in Vero cells. The results showed that expressed shRNA inhibited the virus replication to a significant extent (92%) as determined by the virus titration in cell culture. This outcome demonstrated the effectiveness of RNA interference (RNAi) based mechanism against the IBDV in vitro.
authors: Indian Veterinary Research Institute, Izatnagar, India.
authors: B K
authors: BK
authors: Sajjanar
authors: A
authors: A
authors: Mishra
authors: A
authors: A
authors: Sonawane
authors: C L
authors: CL
authors: Patel
authors: A
authors: A
authors: Saxena
authors: B B
authors: BB
authors: Dash
authors: A
authors: A
authors: Rai
authors: A A
authors: AA
authors: Raut
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D005822:Genetic Vectors / Q000235:genetics; D007243:Infectious bursal disease virus / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000008:administration & dosage* / Q000235:genetics; D014709:Vero Cells; D014764:Viral Proteins / Q000235:genetics*; D014779:Virus Replication / Q000235:genetics*
chemical_list: D034741:RNA, Small Interfering; D014764:Viral Proteins