title: [Determination of the concentration of paeonflorin of the BYHWD and total glycoside in the plasma of rats by reverse phase high performance liquid chromatography and studies on its pharmacokinetics].
abstract: OBJECTIVE
  A RP-HPLC method for the determination of paeonflorin of the BYHWD and total glycoside in the plasma of white rat was reported. The pharmacokinetics of paeonflorin of the BYHWD in white rat were studied.
  
  
  METHODS
  The RP-HPLC was used with the conditions that the column was C18, 4.6 x 250 mm, 5 microm; the detection of wavelengths was set at 230 nm; A solution of methanol-water (33 : 67) was used as the mobile phase; The mobile velocity was 1 mL/min; the pressure of column was (20.71 +/- 0.4173) MPa; the temperature was 35 degrees C. The parameters of pharmacokinetics was dealt with DAS.
  
  
  RESULTS
  The calibration curve was linear in the range of (0.8010-80.10) microg/mL, with r = 0.9999; The average recovery is 99.77%; RSD is 2.183%; The pharmacokinetic parameters of the paeonflorin was that in the compound prescription, t1/2 (alpha) was 2. 569 min; t1/2 (beta) was 64.53 min; K12 was 0.1457 min(-1); K21 was 0.01767 min; K10 was 0.2366 min(-1); in the total glycoside, t1/2 (alpha) was 1.829 min; t1/2 (beta) is 72.27 min; K12 was 0.1587 min(-1); K21 was 0.01708 min(-1); K10 was 0.2127 min(-1);
  
  
  CONCLUSIONS
  The method is rapid,simple and easy to use in determination of Paeonflorin of the BYHWD in the plasma of Mice. The pharmacokinetics of paeonflorin of the BYHWTD in the mice abidied by two compartmental model, as difference as constitution.
authors: Department of Acupuncture and Moxibustion, First Affiliated Hospital, Changsha 410007, China. dkaiwen03@people.com.cn
authors: Kai-Wen
authors: KW
authors: Deng
authors: Fu-yang
authors: FY
authors: He
authors: Wen-Long
authors: WL
authors: Liu
mesh_terms: D000818:Animals; D019540:Area Under Curve; D001565:Benzoates / Q000097:blood* / Q000493:pharmacokinetics*; D001952:Bridged-Ring Compounds / Q000097:blood* / Q000493:pharmacokinetics*; D056148:Chromatography, Reverse-Phase / Q000379:methods; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000032:analysis*; D005260:Female; D005343:Fibrinolytic Agents / Q000008:administration & dosage / Q000032:analysis*; D005960:Glucosides / Q000097:blood* / Q000493:pharmacokinetics*; D006027:Glycosides / Q000008:administration & dosage / Q000032:analysis*; D007275:Injections, Intravenous; D008297:Male; D039821:Monoterpenes; D029596:Paeonia / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
chemical_list: D001565:Benzoates; D001952:Bridged-Ring Compounds; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D005343:Fibrinolytic Agents; D005960:Glucosides; D006027:Glycosides; D039821:Monoterpenes; C015423:peoniflorin