title: Evaluation of electrogenerated chemiluminescence from a neutral Ir(III) complex for quantitative analysis in flowing streams.
abstract: Though recently Ir(III) complexes have attracted much interest in electrochemiluminescent (ECL) analysis due to their high emission in various wavelengths, there were a few studies reported on its analytical applications. In this study, we evaluate the ECL from (pq)(2)Ir(acac) (pq = 2-phenylquinolate, acac = acetylacetonate) for the use in flow injection analysis. An aqueous solution of the analyte and (pq)(2)Ir(acac) passes through the reaction/observation cell, and then ECL reaction is generated by electrochemical initiation on the analyte and (pq)(2)Ir(acac). Tri-n-propylamine (TPrA) is used as a representative analyte for evaluation. Additionally, a comparison is made of the relative ECL intensities obtained for a variety of analytes including oxalate, amino acids, aliphatic amines, and NADH. The (pq)(2)Ir(acac) produces efficient ECL upon TPrA exhibiting the limit of detection of 5 nM with a linear range of 3 orders of magnitude in concentration whereas 20 nM is observed in the conventional Ru(bpy)(3)(2+) system. It shows particular sensitivity advantages for oxalate, proline, and tartaric acid. The ECL generation upon various analytes proposes direct applicability of (pq)(2)Ir(acac) as a post-column detection tool.
authors: Department of Chemistry, Seoul National University, Republic of Korea.
authors: Ik-Soo
authors: IS
authors: Shin
authors: Yeung-Tae
authors: YT
authors: Kang
authors: Jin-Kyu
authors: JK
authors: Lee
authors: Hasuck
authors: H
authors: Kim
authors: Tae Hyun
authors: TH
authors: Kim
authors: Jong Seung
authors: JS
authors: Kim
mesh_terms: D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000379:methods; D017022:Flow Injection Analysis / Q000379:methods; D006863:Hydrogen-Ion Concentration; D006885:Hydroxybutyrates / Q000737:chemistry; D007495:Iridium / Q000737:chemistry*; D008163:Luminescent Measurements / Q000379:methods*; D010422:Pentanones / Q000737:chemistry; D011437:Propylamines / Q000032:analysis
chemical_list: D056831:Coordination Complexes; D006885:Hydroxybutyrates; D010422:Pentanones; D011437:Propylamines; C049529:acetyl acetonate; D007495:Iridium