title: Detection of small RNAs and microRNAs using deep sequencing technology.
abstract: Small RNAs (sRNAs) have made a large impact on many recent scientific discoveries. MicroRNAs (miRNAs) are a type of sRNA molecule and, although usually just 20-22 nucleotides in length, they are potent regulators of gene expression. Therefore, identification of miRNAs and profiling their abundance are fundamental to understanding an organism's or tissue's gene regulatory network. Next-generation sequencing methods have allowed researchers to quickly sequence and profile sRNA populations. This chapter describes a cloning procedure to identify the sRNAs or miRNAs present in an RNA sample.
authors: Department of Plant Sciences, University of Cambridge, Cambridge, UK. erh45@cam.ac.uk
authors: Ericka R
authors: ER
authors: Havecker
mesh_terms: D003001:Cloning, Molecular; D020869:Gene Expression Profiling / Q000379:methods; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D035683:MicroRNAs / Q000032:analysis*; D058727:RNA, Small Untranslated / Q000032:analysis*; D017423:Sequence Analysis, RNA / Q000379:methods*
chemical_list: D035683:MicroRNAs; D058727:RNA, Small Untranslated