title: Cell cycle-dependent phosphorylation of pRb-like protein in root meristem cells of Vicia faba.
abstract: The retinoblastoma tumor suppressor protein (pRb) regulates cell cycle progression by controlling the G1-to-S phase transition. As evidenced in mammals, pRb has three functionally distinct binding domains and interacts with a number of proteins including the E2F family of transcription factors, proteins with a conserved LxCxE motif (D-type cyclin), and c-Abl tyrosine kinase. CDK-mediated phosphorylation of pRb inhibits its ability to bind target proteins, thus enabling further progression of the cell cycle. As yet, the roles of pRb and pRb-binding factors have not been well characterized in plants. By using antibody which specifically recognizes phosphorylated serines (S807/811) in the c-Abl tyrosine kinase binding C-domain of human pRb, we provide evidence for the cell cycle-dependent changes in pRb-like proteins in root meristems cells of Vicia faba. An increased phosphorylation of this protein has been found correlated with the G1-to-S phase transition.
authors: Department of Cytophysiology, University of Łódź, Łódź, Poland. justpoli@poczta.onet.pl
authors: Justyna Teresa
authors: JT
authors: Polit
authors: Andrzej
authors: A
authors: Kaźmierczak
authors: Aurelia
authors: A
authors: Walczak-Drzewiecka
mesh_terms: D000918:Antibody Specificity; D015153:Blotting, Western; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000378:metabolism; D007150:Immunohistochemistry; D018519:Meristem / Q000166:cytology / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation; D059828:Plant Cells / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D018517:Plant Roots / Q000166:cytology / Q000378:metabolism; D017434:Protein Structure, Tertiary; D016160:Retinoblastoma Protein / Q000378:metabolism*; D012694:Serine / Q000378:metabolism; D031307:Vicia faba / Q000166:cytology / Q000378:metabolism*
chemical_list: D018797:Cell Cycle Proteins; D010750:Phosphoproteins; D010940:Plant Proteins; D016160:Retinoblastoma Protein; D012694:Serine