title: Genomic profiling of papillary renal cell tumours identifies small regions of DNA alterations: a possible role of HNF1B in tumour development.
abstract: OBJECTIVE
  Papillary renal cell tumours (RCT) are characterized by specific trisomies. The aim of this study was to identify small regions of duplication marking putative tumour genes.
  
  
  RESULTS
  Full-tiling path bacterial artificial chromosome (BAC) array hybridization of 20 papillary RCTs confirmed the duplication of chromosomes 7 and 17 and also displayed smaller regions of gains/amplifications of 1.3-13.1 Mb in size. Detailed analysis showed a microamplification of BAC clones containing the MET at the 7q31.2 and also amplification of a DNA segment harbouring the transcription factor hepatocyte nuclear factor 1 beta (HNF1B) at chromosome 17q12. Nuclear expression of HNF1B protein was detected in 38 of 67 papillary RCTs, in five of five mucinous tubular and spindle cell carcinomas (MTSCC) and five of five metanephric adenomas by immunohistochemistry. Moreover, nine nephrogenic rests containing tubular differentiated structures and all 14 and five precursor lesions associated with papillary RCTs and MTSCCs, respectively, showed strong nuclear positivity when compared to the expression level in proximal tubules of the corresponding normal kidney.
  
  
  CONCLUSIONS
  Our findings indicate a role of HNF1B in association with the high frequency of chromosome 17q duplication in the development of papillary RCTs and MTSCCs as well as in their precursor lesions.
authors: Laboratory of Molecular Oncology, Medical Faculty, Ruprecht-Karls-University, Heidelberg, Germany.
authors: Adrianna
authors: A
authors: Szponar
authors: Maria V
authors: MV
authors: Yusenko
authors: Roland
authors: R
authors: Kuiper
authors: Ad Geurts
authors: AG
authors: van Kessel
authors: Gyula
authors: G
authors: Kovacs
mesh_terms: D002288:Adenocarcinoma, Mucinous / Q000235:genetics / Q000503:physiopathology; D000236:Adenoma / Q000235:genetics / Q000503:physiopathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002292:Carcinoma, Renal Cell / Q000235:genetics* / Q000503:physiopathology; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D002886:Chromosomes, Human, Pair 17 / Q000235:genetics; D002897:Chromosomes, Human, Pair 7 / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics*; D005260:Female; D020440:Gene Duplication / Q000235:genetics*; D020869:Gene Expression Profiling; D051539:Hepatocyte Nuclear Factor 1-beta / Q000502:physiology*; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics* / Q000503:physiopathology; D008297:Male; D008875:Middle Aged; D012189:Retrospective Studies; D014314:Trisomy
chemical_list: D004273:DNA, Neoplasm; C496495:HNF1B protein, human; D051539:Hepatocyte Nuclear Factor 1-beta