title: Mechanism of gene transfection by polyamidoamine (PAMAM) dendrimers modified with ornithine residues.
abstract: The aim of this study was to prepare and investigate the mechanism of uptake of the dendriplexes prepared with ornithine-conjugated polyamidoamine (PAMAM) G4 dendrimers. Ornithine-conjugated PAMAMG4 dendrimers were prepared by Fmoc synthesis. A comparative transfection study in NCI H157G cells and polyamine transport-deficient cell line NCI H157R was performed to confirm the role of the polyamine transporter system (PAT) in the dendriplex uptake. Transfection efficiency significantly increased with increase in generation number and extent of ornithine conjugation. Transfection efficiency of the PAMAMG4-ORN60 dendrimers significantly decreased in presence of excess of ornithine (P < 0.05) and paraquat (P < 0.01) but not of PAMAMG4 dendrimers. Transfection efficiency of PAMAMG4-ORN60 was significantly low in NCI H157R (31.66 ± 3.95%, RFU: 17.87 ± 1.34) as compared to NCI H157G cell line (63.07 ± 6.8%, relative fluorescence units (RFU): 23.28 ± 0.66). Results indicate the role of PAT in addition to charge-mediated endocytosis in the internalization of ornithine-conjugated PAMAMG4 dendrimers. Cytotoxicity analysis (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay) in human embryonic kidney cell line (HEK) 293T cells showed that the dendriplexes were non-toxic at N/P 10.
authors: Department of Pharmaceutical Sciences, Irma Lerma Rangel College of Pharmacy, Texas A&M Health Science Center, Kingsville, TX, USA.
authors: Ajay
authors: A
authors: Kumar
authors: Venkata K
authors: VK
authors: Yellepeddi
authors: Kiran K
authors: KK
authors: Vangara
authors: Kevin B
authors: KB
authors: Strychar
authors: Srinath
authors: S
authors: Palakurthi
mesh_terms: D001692:Biological Transport; D002460:Cell Line; D004247:DNA / Q000008:administration & dosage*; D050091:Dendrimers / Q000737:chemistry* / Q000493:pharmacokinetics / Q000633:toxicity; D004705:Endocytosis; D005453:Fluorescence; D057809:HEK293 Cells; D006801:Humans; D009757:Nylons / Q000737:chemistry* / Q000493:pharmacokinetics / Q000633:toxicity; D009952:Ornithine / Q000737:chemistry*; D010269:Paraquat / Q000737:chemistry; D011073:Polyamines / Q000378:metabolism; D014162:Transfection
chemical_list: D050091:Dendrimers; D009757:Nylons; C520550:PAMAM-G4; D011073:Polyamines; D004247:DNA; D009952:Ornithine; D010269:Paraquat