title: The characterization of two peroxiredoxin genes in Dunaliella viridis provides insights into antioxidative response to salt stress.
abstract: Peroxiredoxins (Prxs), a group of antioxidant enzymes, are an important component of the oxidative defense system and have been demonstrated to function as peroxidases, sensors of H(2)O(2)-mediated signaling and/or chaperones. In this study, a cDNA library was constructed from a halotolerant alga, Dunaliella viridis, and was used in a functional complementation screen for antioxidative genes in an oxidative sensitive yeast mutant. Two Prx genes, DvPrx1 and DvPrx2, were obtained from this screen. These two genes were classified as type II Prx and 2-Cys Prx based on amino acid sequence and phylogenetic analysis. When over-expressed in yeast cells, both Prx genes were able to confer better oxidative tolerance and decrease the level of reactive oxygen species (ROS). Subcellular localization experiments in tobacco cells revealed that both DvPrx1 and DvPrx2 were localized in the cytosol. The transcription of DvPrx1 and DvPrx2 can be induced by hypersalinity shock, but is not obviously affected by treatment with high levels of oxidant. Our results shed light on the function and regulation of Prx genes from Dunaliella and their potential roles in salt tolerance.
authors: Shanghai Key Laboratory of Bio-Energy Crops, School of Life Sciences, Shanghai University, 99 Shangda Road, 200444, Shanghai, People's Republic of China.
authors: Huijuan
authors: H
authors: Yuan
authors: Xiangzong
authors: X
authors: Meng
authors: Qiang
authors: Q
authors: Gao
authors: Wufei
authors: W
authors: Qu
authors: Tengjiao
authors: T
authors: Xu
authors: Zhengkai
authors: Z
authors: Xu
authors: Rentao
authors: R
authors: Song
mesh_terms: D000595:Amino Acid Sequence; D000975:Antioxidants / Q000378:metabolism*; D000460:Chlorophyta / Q000235:genetics* / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D015723:Gene Library; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D018384:Oxidative Stress; D054464:Peroxiredoxins / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D055049:Salt Tolerance; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000494:pharmacology*; D013312:Stress, Physiological; D059467:Transcriptome
chemical_list: D000975:Antioxidants; D010940:Plant Proteins; D017382:Reactive Oxygen Species; D012965:Sodium Chloride; D054464:Peroxiredoxins