title: Characterization of Enterobius vermicularis in a human population, employing a molecular-based method from adhesive tape samples.
abstract: Human infection with the parasitic nematode Enterobius vermicularis occurs worldwide, particularly in children. Although its prevalence may exceed 35% in some parts of the world, molecular studies of E. vermicularis in humans are limited. The aim of the present study was to investigate the genetic variation within E. vermicularis in a human population. For this purpose, 77 adhesive tape samples taken from Greek children infested with E. vermicularis were tested. New primers were designed to amplify a segment of the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene of E. vermicularis from adhesive tape samples. Thirty-six amplicons were sequenced and eleven different haplotypes were identified. All sequences clustered within the type previously characterized (type B), only reported to date from captive chimpanzees. To the best of our knowledge, this is the first study of E. vermicularis genotypes from a human population.
authors: Department of Microbiology, Medical School, National University of Athens, Mikras Asias 75, Athens 115-27, Greece. epiper@med.uoa.gr
authors: Evangelia-Theophano
authors: ET
authors: Piperaki
authors: Gregory
authors: G
authors: Spanakos
authors: Giannoula
authors: G
authors: Patsantara
authors: Evdokia
authors: E
authors: Vassalou
authors: Nikolaos
authors: N
authors: Vakalis
authors: Athanassios
authors: A
authors: Tsakris
mesh_terms: D000269:Adhesives; D000818:Animals; D002648:Child; D017931:DNA Primers / Q000235:genetics; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000145:classification / Q000235:genetics; D017229:Enterobiasis / Q000175:diagnosis / Q000469:parasitology*; D004757:Enterobius / Q000235:genetics*; D005843:Geography; D006115:Greece; D015801:Helminth Proteins / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA
chemical_list: D000269:Adhesives; D017931:DNA Primers; D018509:DNA, Helminth; D015801:Helminth Proteins; D003576:Electron Transport Complex IV