title: Production of N-acetyl-D-neuraminic acid by use of an efficient spore surface display system.
abstract: Production of N-acetyl-D-neuraminic acid (Neu5Ac) via biocatalysis is traditionally conducted using isolated enzymes or whole cells. The use of isolated enzymes is restricted by the time-consuming purification process, whereas the application of whole cells is limited by the permeability barrier presented by the microbial cell membrane. In this study, a novel type of biocatalyst, Neu5Ac aldolase presented on the surface of Bacillus subtilis spores, was used for the production of Neu5Ac. Under optimal conditions, Neu5Ac at a high concentration (54.7 g liter⁻¹) and a high yield (90.2%) was obtained under a 5-fold excess of pyruvate over N-acetyl-D-mannosamine. The novel biocatalyst system, which is able to express and immobilize the target enzyme simultaneously on the surface of B. subtilis spores, represents a suitable alternative for value-added chemical production.
authors: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, People's Republic of China.
authors: Xiaoman
authors: X
authors: Xu
authors: Chao
authors: C
authors: Gao
authors: Xifeng
authors: X
authors: Zhang
authors: Bin
authors: B
authors: Che
authors: Cuiqing
authors: C
authors: Ma
authors: Jianhua
authors: J
authors: Qiu
authors: Fei
authors: F
authors: Tao
authors: Ping
authors: P
authors: Xu
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods; D019158:N-Acetylneuraminic Acid / Q000378:metabolism*; D007652:Oxo-Acid-Lyases / Q000235:genetics* / Q000378:metabolism*; D013171:Spores, Bacterial / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism*
chemical_list: D007652:Oxo-Acid-Lyases; C018728:N-acetylneuraminate lyase; D019158:N-Acetylneuraminic Acid