title: Identification of salt-tolerant gene HOG1 in Torulopsis versatilis.
abstract: To understand the osmo-adaptation mechanism in Torulopsis versatilis (T), we investigated the salt-tolerant gene HOG1 from the wild-type and a salt-tolerant mutant strain (T5) constructed using genome shuffling. The HOG1 genes from T and T5 were sequenced and revealed several mutations had occurred. The expression level of T5HOG1 was stronger than that of THOG1, indicating a reason for the increase of salt-tolerance in T. versatilis. Moreover, overexpression of T5HOG1 and THOG1 improved the tolerance of salt in Saccharomyces cerevisiae. Identification and overexpression of THOG1 and T5HOG1 from the wild-type T. versatilis and the mutant T. versatilis, respectively, play an important role for the osmo-adaption mechanism of the T. versatilis used in soy-sauce fermentation.
authors: Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Ministry of Education, Tianjin, China.
authors: Xiao-Xong
authors: XX
authors: Cao
authors: Meng
authors: M
authors: Meng
authors: Yuan-Yuan
authors: YY
authors: Wang
authors: Chun-Ling
authors: CL
authors: Wang
authors: Li-Hua
authors: LH
authors: Hou
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D041221:Candida glabrata / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000502:physiology / Q000276:immunology; D002177:Candidiasis; D004252:DNA Mutational Analysis; D032621:DNA Shuffling; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D025141:Drug Resistance, Fungal; D020869:Gene Expression Profiling; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation; D009997:Osmotic Pressure; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012492:Salts / Q000633:toxicity*; D017422:Sequence Analysis, DNA; D013312:Stress, Physiological
chemical_list: D004271:DNA, Fungal; D012492:Salts; D020928:Mitogen-Activated Protein Kinases