title: Antigenic and cross-protection studies of biotype 1 and biotype 2 isolates of Yersinia ruckeri in rainbow trout, Oncorhynchus mykiss (Walbaum).
abstract: OBJECTIVE
  The study investigated antigen characteristics of biotype (bt) 1 and bt 2 isolates of Yersinia ruckeri.
  
  
  RESULTS
  The cell surface characteristics of Y. ruckeri were compared for their antigenic characteristics using polyclonal antibodies that revealed that both biotypes had a homogenous whole-cell protein antigenic profile. Notable differences in the antigenic properties were observed in the lipopolysaccharide profile of both biotypes. Two iron-regulated outer membrane proteins (IROMP) of c.90 and 100 kDa were shown to be major specific antigens. The results demonstrate for the first time differences in antigens between bt 1 and bt 2 isolates of serotype O1 isolates of Y. ruckeri. The protection induced in rainbow trout by a commercial monovalent, and bivalent inactivated vaccine was tested with the outcome that the ability of isolates to cause mortality in vaccinated fish varied with geographical location. In this context, vaccination studies suggested that the O antigen was the dominant immunogenic molecule involved in protection against the disease.
  
  
  CONCLUSIONS
  The O antigen of Y. ruckeri was the dominant immunogenic molecule involved in the protection of rainbow trout against enteric redmouth disease.
  
  
  CONCLUSIONS
  There are distinct phenotypic and antigenic differences in Y. ruckeri bt 1 and bt 2 with O antigen recognized as the dominant immunogenic molecule. The data have significance in explaining the lack of success of the earlier monovalent vaccine and demonstrate the effectiveness of the newer bivalent vaccine.
authors: School of Life Sciences, Heriot-Watt University, Edinburgh, UK.
authors: J W
authors: JW
authors: Tinsley
authors: A R
authors: AR
authors: Lyndon
authors: B
authors: B
authors: Austin
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis* / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis / Q000276:immunology; D001428:Bacterial Vaccines / Q000276:immunology; D003429:Cross Reactions; D005393:Fish Diseases / Q000276:immunology* / Q000382:microbiology* / Q000517:prevention & control; D008070:Lipopolysaccharides / Q000032:analysis / Q000276:immunology; D019081:O Antigens / Q000276:immunology; D017686:Oncorhynchus mykiss; D015009:Yersinia Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D044124:Yersinia ruckeri / Q000145:classification / Q000276:immunology* / Q000472:pathogenicity
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D008070:Lipopolysaccharides; D019081:O Antigens