title: The FUN30 chromatin remodeler, Fft3, protects centromeric and subtelomeric domains from euchromatin formation.
abstract: The chromosomes of eukaryotes are organized into structurally and functionally discrete domains. This implies the presence of insulator elements that separate adjacent domains, allowing them to maintain different chromatin structures. We show that the Fun30 chromatin remodeler, Fft3, is essential for maintaining a proper chromatin structure at centromeres and subtelomeres. Fft3 is localized to insulator elements and inhibits euchromatin assembly in silent chromatin domains. In its absence, euchromatic histone modifications and histone variants invade centromeres and subtelomeres, causing a mis-regulation of gene expression and severe chromosome segregation defects. Our data strongly suggest that Fft3 controls the identity of chromatin domains by protecting these regions from euchromatin assembly.
authors: Department of Biosciences and Medical Nutrition, Center for Biosciences, Karolinska Institutet, Huddinge, Sweden.
authors: Annelie
authors: A
authors: Strålfors
authors: Julian
authors: J
authors: Walfridsson
authors: Hasanuzzaman
authors: H
authors: Bhuiyan
authors: Karl
authors: K
authors: Ekwall
mesh_terms: D002503:Centromere / Q000378:metabolism*; D042002:Chromatin Assembly and Disassembly / Q000235:genetics*; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics* / Q000378:metabolism*; D022041:Euchromatin / Q000378:metabolism*; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D023061:Gene Order; D006657:Histones / Q000235:genetics / Q000378:metabolism; D038101:Insulator Elements / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics; D012568:Schizosaccharomyces / Q000201:enzymology* / Q000235:genetics*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics* / Q000378:metabolism*; D016615:Telomere / Q000378:metabolism*
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D022041:Euchromatin; C559589:Fft3 protein, S pombe; D006657:Histones; D029702:Schizosaccharomyces pombe Proteins; D012343:RNA, Transfer