title: Reduced activity and cytoplasmic localization of Runx2 is observed in C3h10t1/2 cells overexpressing Tbx3.
abstract: Tbox3, a T-box containing transcription factor, has been reported to negatively regulate osteoblastogenesis. Here, we studied the effects mediated by Tbx3 on the master osteogenic transcription factor Runx2 in C3h10t1/2 cells. Dual-luciferase assay results showed that Tbx3 interferes in the Runx2-mediated activation of osteopontin promoter by abrogating Runx2 activity. Immunofluorescence study demonstrated that Runx2 was simultaneously localized in the nucleus and the cytoplasm of Tbx3-overexpressing cells. The results obtained in this study indicate that Tbx3 abrogates Runx2 activity and elevated expression levels of Tbx3 in the cells can result in mislocalization of Runx2.
authors: Institute of Genetics and Cell Biology, Northeast Normal University, Changchun, China.
authors: Vishwa
authors: V
authors: Deepak
authors: Zhongli
authors: Z
authors: Zhang
authors: Lingyi
authors: L
authors: Meng
authors: Xianlu
authors: X
authors: Zeng
authors: Wenguang
authors: W
authors: Liu
mesh_terms: D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics / Q000378:metabolism*; D003593:Cytoplasm / Q000235:genetics / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D008156:Luciferases; D051379:Mice; D053495:Osteopontin / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D020825:T-Box Domain Proteins / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
chemical_list: D050659:Core Binding Factor Alpha 1 Subunit; C493716:Runx2 protein, mouse; D020825:T-Box Domain Proteins; C491460:Tbx3 protein, mouse; D053495:Osteopontin; D008156:Luciferases