title: Repeated fed-batch fermentation using biosensor online control for citric acid production by Yarrowia lipolytica.
abstract: Biosensor-controlled substrate feeding was used in a citric acid production process with the yeast strain Yarrowia lipolytica H222 with glucose as the carbon source. The application of an online glucose biosensor measurement facilitated the performance of long-time repeated fed-batch process with automated bioprocess control. Ten cycles of repeated fed-batch fermentation were carried out in order to validate both the stability of the microorganism for citric acid production and the robustness of the glucose biosensor in a long-time experiment. In the course of this fermentation with a duration of 553 h, a slight loss of productivity from 1.4 g/(L×h) to 1.1 g/(L×h) and of selectivity for citric acid from 91% to 88% was observed. The glucose biosensor provided 6,227 measurements without any loss of activity.
authors: Helmholtz Centre for Environmental Research-UFZ, Centre for Environmental Biotechnology, Leipzig, Germany.
authors: Lucie
authors: L
authors: Moeller
authors: Mario
authors: M
authors: Grünberg
authors: Andreas
authors: A
authors: Zehnsdorf
authors: Andreas
authors: A
authors: Aurich
authors: Thomas
authors: T
authors: Bley
authors: Beate
authors: B
authors: Strehlitz
mesh_terms: D019149:Bioreactors / Q000382:microbiology*; D015374:Biosensing Techniques / Q000379:methods*; D019343:Citric Acid / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000032:analysis / Q000378:metabolism*; D025062:Yarrowia / Q000378:metabolism*
chemical_list: D019343:Citric Acid; D005947:Glucose