title: Use of a Mycoplasma suis-PCR protocol for screening a population of captive peccaries (Tayassu tajacu and Tayassu pecari).
abstract: Mycoplasma suis is a hemotropic bacteria of red blood cells and the causative agent of swine eperythrozoonosis. Diagnosis of infection may be reached by direct examination of blood smears; however, the use of polymerase chain reaction (PCR) of the 16S RNA gene of M. suis improves the sensitivity and specificity of detection. The aim of this study was to screen peccaries (Tayassu tajacu and T. pecari) for M. suis infection using a specific conventional PCR. A total of 28 blood samples from captive collared and white-lipped peccaries were collected, DNA extracted and a specific M. suis PCR assay performed. All samples were negatives by both blood smear examination and PCR testing. To verify the presence of amplifiable DNA, PCR for beta-actin gene was performed in all samples. This study was part of an active surveillance program, which is crucial for monitoring animal health status, particularly in wildlife species.
authors: Departamento de MedicinaVeterinária Preventiva, Universidade Estadual de Londrina, Londrina, Brazil. vieirarfc@gmail.com
authors: Rafael Felipe da Costa
authors: RF
authors: Vieira
authors: Marcelo Beltrão
authors: MB
authors: Molento
authors: Ana Marcia Sa
authors: AM
authors: Guimarães
authors: Andrea Pires Dos
authors: AP
authors: Santos
authors: Marcelo
authors: M
authors: Bonat
authors: Manoel Lucas
authors: ML
authors: Javorouski
authors: Luciene
authors: L
authors: Popp
authors: Leonilda Correia Dos
authors: LC
authors: Santos
authors: Wanderlei
authors: W
authors: Moraes
authors: Zalmir Silvino
authors: ZS
authors: Cubas
authors: Thállitha Samih Wischral Jayme
authors: TS
authors: Vieira
authors: Odilon
authors: O
authors: Vidotto
authors: Ivan Roque Barros
authors: IR
authors: Filho
authors: Alexander Welker
authors: AW
authors: Biondo
authors: Joanne Belle
authors: JB
authors: Messick
mesh_terms: D000818:Animals; D001188:Artiodactyla / Q000382:microbiology*; D009174:Mycoplasma / Q000235:genetics* / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods