title: The dominating role of N-deacetylase/N-sulfotransferase 1 in forming domain structures in heparan sulfate.
abstract: Heparan sulfate (HS) is a highly sulfated polysaccharide participated in essential physiological functions from regulating cell growth to blood coagulation. HS contains sulfated domains known as N-S domains and low sulfate domains known as N-Ac domains. The distribution of the domain structures is likely governed by the action of glucosaminyl N-deacetylase/N-sulfotransferase (NDST). Here, we sought to determine the substrate specificity of NDST using model substrates and recombinant NDST protein. We discovered that NDST-1 carries out the modification in a highly ordered fashion. The enzyme sulfates the substrate from the nonreducing end toward the reducing end consecutively, leading to the product with a cluster of N-sulfo glucosamine residues. Furthermore, a preexisting N-sulfo glucosamine residue prevents the action of NDST-1 at the residues immediately located at the nonreducing end, allowing the formation of an N-Ac domain. Our results provide the long sought evidence for understanding the formation of sulfated versus nonsulfated domains in the HS isolated from cells and tissues. The study demonstrates the regulating role of NDST-1 in mapping the sulfation patterns of HS.
authors: Division of Medicinal Chemistry and Natural Products, Eshelman School of Pharmacy, University of North Carolina, Chapel Hill, North Carolina 27599, USA.
authors: Juzheng
authors: J
authors: Sheng
authors: Renpeng
authors: R
authors: Liu
authors: Yongmei
authors: Y
authors: Xu
authors: Jian
authors: J
authors: Liu
mesh_terms: D000818:Animals; D002236:Carbohydrate Conformation; D006497:Heparan Sulfate / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D017434:Protein Structure, Tertiary; D051381:Rats; D015238:Sulfotransferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D006497:Heparan Sulfate; D015238:Sulfotransferases; C021350:heparitin sulfotransferase