title: RIP: an mRNA localization technique.
abstract: A detailed understanding of post-transcriptional gene expression is necessary to correlate the different elements involved in the many levels of RNA-protein interactions that are needed to coordinate the cellular biomolecular machinery. The profile of mRNA, a major component of this machinery, can be examined after isolation from specific RNA-binding proteins (RBPs). RIP-Chip or ribonomic profiling is a versatilein vivo technique that has been widely used to study post-transcriptional gene regulation and the localization of mRNA. Here we elaborately detail the methodology for mRNA isolation using RBP immunoprecipitation (RIP) as a primary approach. Specific antibodies are used to target RBPs, which are then used to capture the associated mRNA.
authors: Department of Nanobioscience, University at Albany, Albany, NY, USA.
authors: Sabarinath
authors: S
authors: Jayaseelan
authors: Francis
authors: F
authors: Doyle
authors: Salvatore
authors: S
authors: Currenti
authors: Scott A
authors: SA
authors: Tenenbaum
mesh_terms: D000818:Animals; D055910:Antibodies, Immobilized / Q000276:immunology / Q000378:metabolism; D000918:Antibody Specificity; D002457:Cell Extracts; D019295:Computational Biology; D006367:HeLa Cells; D006801:Humans; D047468:Immunoprecipitation / Q000379:methods*; D020014:K562 Cells; D008280:Magnetics; D008863:Microspheres; D009419:Nerve Tissue Proteins / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D034443:RNA Transport; D012333:RNA, Messenger / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D012261:Ribonucleoproteins / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D013205:Staphylococcal Protein A / Q000378:metabolism
chemical_list: D055910:Antibodies, Immobilized; D002457:Cell Extracts; C029152:G-substrate; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D012261:Ribonucleoproteins; D013205:Staphylococcal Protein A