title: Pamidronate and zoledronate effects in the increment of bone mineral density and histomorphometry in rats.
abstract: OBJECTIVE
  To compare increment of bone mineral density (BMD) with pamidronate, zoledronate and the isolated effect of proteinous diet in undernourished oophorectomized and non-oophorectomized female rats, besides validating BMD's indexes.
  
  
  METHODS
  60 young female Lewis rats were divided into five experimental groups and a control group, oophorectomized and non-oophorectomized. The administration of drugs were submitted to proteinous and aproteinous diets. The variables analyzed were weight, bone densitometry, histomorphometry and biochemical evolution.
  
  
  RESULTS
  In weight evaluation, the first interval showed a statistically meaningful increase in oophorectomized sample. In densitometry evaluation, the first interval showed statistically meaningful decrease in four medicated groups and third showed a statistically meaningful increase in 2 non-oophorectomized groups. In laboratory evaluation, there were an increase of total proteins and globulin, decrease of alkaline phosphatase, phosphorus and calcium (except for the oophorectomized) in four medicated groups and increase of phosphorus and calcium in 2 not medicated groups. In histomorphometric evaluation, the oophorectomized groups had smaller increment of BMD.
  
  
  CONCLUSIONS
  The pamidronate and zoledronate have shown effectives in the increment of BMD. The proteinous diet itself possesses therapeutic effect in BMD though not significant compared with medicated animals. The results of histomorphometry allow validating BMD's indexes in this experimental model.
authors: Department of Clinical Medicine, Faculty of Medicine of Uninga, Maringa, PR, Brazil.
authors: Antônio Fiel
authors: AF
authors: Cruz Júnior
authors: Carlos
authors: C
authors: Buchpiguel
authors: Roberto
authors: R
authors: Guarniero
authors: Antonio
authors: A
authors: Barbieri
mesh_terms: D000818:Animals; D015519:Bone Density / Q000187:drug effects*; D050071:Bone Density Conservation Agents / Q000494:pharmacology*; D003720:Densitometry; D004044:Dietary Proteins / Q000008:administration & dosage; D004164:Diphosphonates / Q000494:pharmacology*; D005260:Female; D007093:Imidazoles / Q000494:pharmacology*; D044342:Malnutrition; D010052:Ovariectomy; D000077268:Pamidronate; D011897:Random Allocation; D051381:Rats; D011917:Rats, Inbred Lew; D000077211:Zoledronic Acid
chemical_list: D050071:Bone Density Conservation Agents; D004044:Dietary Proteins; D004164:Diphosphonates; D007093:Imidazoles; D000077211:Zoledronic Acid; D000077268:Pamidronate