title: Examination of camptothecin and 10-hydroxycamptothecin in Camptotheca acuminata plant and cell culture, and the affected yields under several cell culture treatments.
abstract: Camptothecin and its derivatives are monoterpenoid indole alkaloids exhibiting significant anti-tumor actions. With the aim of improving the production of these pharmaceuticals, the contents of camptothecin and 10-hydroxycamptothecin in different tissues including roots, stems, leaves, young flower buds, opening flowers, fading flowers and seeds from Camptotheca acuminata, were investigated. The young flower buds had the highest alkaloid concentrations (camptothecin, 2.46 mg/g of dry weight; 10-hydroxycamptothecin, 1.41 mg/g of dry weight). Callus showed lower concentrations but it should also be considered as a potential source of these pharmaceuticals. In the present study, the growth rate of Camptotheca acuminata cells in culture did not correlate with contents of camptothecin and 10-hydroxycamptothecin. Alkaloid accumulation by cells under various treatments (heavy metal ions, UV-B), methyl-jasmonate, abscisic acid, salicylic acid and hydrogen peroxide was examined, and the most notable effects appeared in the cells induced by UV-B light (which showed an 11-fold increase in camptothecin concentration) and by salicylic acid (which showed a 25-fold increase in 10-hydroxycamptothecin concentration). These results are significant in the context of the production of both pharmaceuticals.
authors: State Key Laboratory of Genetic Engineering, School of Life Sciences, Morgan-Tan International Center for Life Sciences, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, Fudan University, Shanghai 200433, PR China.
authors: Yan
authors: Y
authors: Pi
authors: Keji
authors: K
authors: Jiang
authors: Rong
authors: R
authors: Hou
authors: Yifu
authors: Y
authors: Gong
authors: Juan
authors: J
authors: Lin
authors: Xiaofen
authors: X
authors: Sun
authors: Kexuan
authors: K
authors: Tang
mesh_terms: D000470:Alkaloids / Q000302:isolation & purification; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification*; D029769:Camptotheca / Q000737:chemistry / Q000166:cytology / Q000254:growth & development; D002166:Camptothecin / Q000031:analogs & derivatives* / Q000302:isolation & purification; D018929:Cell Culture Techniques / Q000379:methods*; D003470:Culture Media; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification*
chemical_list: D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D003470:Culture Media; D004365:Drugs, Chinese Herbal; C028098:10-hydroxycamptothecin; D002166:Camptothecin