title: Characterization and biocompatibility of chitosan nanocomposites.
abstract: Chitosan nanocomposites were prepared from chitosan and gold nanoparticles (AuNPs) or silver nanoparticles (AgNPs) of ∼5 nm size. Transmission electron microscopy (TEM) showed the NPs in chitosan did not aggregate until higher concentrations (120-240 ppm). Atomic force microscopy (AFM) demonstrated that the nanocrystalline domains on chitosan surface were more evident upon addition of AuNPs (60 ppm) or AgNPs (120 ppm). Both nanocomposites showed greater elastic modulus, higher glass transition temperature (T(g)) and better cell proliferation than the pristine chitosan. Additionally, chitosan-Ag nanocomposites had antibacterial ability against Staphylococcus aureus. The potential of chitosan-Au nanocomposites as hemostatic wound dressings was evaluated in animal (rat) studies. Chitosan-Au was found to promote the repair of skin wound and hemostasis of severed hepatic portal vein. This study indicated that a small amount of NPs could induce significant changes in the physicochemical properties of chitosan, which may increase its biocompatibility and potential in wound management.
authors: Institute of Polymer Science and Engineering, National Taiwan University, Taipei, Taiwan. shhsu@ntu.edu.tw
authors: Shan-hui
authors: SH
authors: Hsu
authors: Yu-Bin
authors: YB
authors: Chang
authors: Ching-Lin
authors: CL
authors: Tsai
authors: Keng-Yen
authors: KY
authors: Fu
authors: Shu-Hua
authors: SH
authors: Wang
authors: Hsiang-Jung
authors: HJ
authors: Tseng
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006046:Gold / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D008297:Male; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D018625:Microscopy, Atomic Force; D046529:Microscopy, Electron, Transmission; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D012834:Silver / Q000737:chemistry / Q000494:pharmacology; D012867:Skin / Q000187:drug effects / Q000293:injuries; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development; D044366:Transition Temperature; D014945:Wound Healing / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D012834:Silver; D006046:Gold; D048271:Chitosan