title: Effect of different forms of anionic nanoclays on cytotoxicity.
abstract: Anionic nanoclays, so-called layered double hydroxide (LDH) nanoparticles, have been extensively applied as drug delivery systems, since they efficiently enter cells via endocytosis pathway and possess controlled release property. However, the stability of LDHs varies, depending on the type of interlayer anions, which can also affect their toxicity. In this study, we investigated the effects of two different forms of LDH, carbonate form (MgAl-LDH-CO3) and chloride form (MgAl-LDH-Cl), on cytotoxicity in human lung epithelial cells. The result showed that MgAl-LDH-Cl was more easily dissolved into metal ions under simulated lysosomal (pH 4.5) and body fluid (pH 7.4) conditions than did MgAl-LDH-CO3. According to cytotoxicity evaluation, MgAl-LDH-CO3 exhibited high toxicity compared with MgAl-LDH-Cl in terms of induction of oxidative stress, apoptosis and membrane damage. These results suggest that easily dissoluble MgAl-LDH-Cl has low cytotoxicity, while high stability of MgAl-LDH-CO3 is correlated to elevated cytotoxicity.
authors: Department of Food Science and Technology, Seoul Women's University, Seoul 139-774, Korea.
authors: Miri
authors: M
authors: Baek
authors: In-Sun
authors: IS
authors: Kim
authors: Jin
authors: J
authors: Yu
authors: Hae Eun
authors: HE
authors: Chung
authors: Jin-Ho
authors: JH
authors: Choy
authors: Soo-Jin
authors: SJ
authors: Choi
mesh_terms: D000838:Anions; D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects; D016503:Drug Delivery Systems; D004355:Drug Stability; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000633:toxicity* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D036103:Nanotechnology; D018384:Oxidative Stress / Q000187:drug effects
chemical_list: D000838:Anions; D007770:L-Lactate Dehydrogenase