title: Oleanolic acid and ursolic acid derived from Cornus officinalis Sieb. et Zucc. suppress epidermal growth factor- and phorbol ester-induced MUC5AC mucin production and gene expression from human airway epithelial cells.
abstract: In this study, the effects of oleanolic acid and ursolic acid on MUC5AC mucin production and gene expression induced by epidermal growth factor (EGF) and phorbol 12-myristate 13-acetate (PMA) from human airway epithelial cells were investigated. Confluent NCI-H292 cells were pretreated with each agent for 30 min and then stimulated with EGF and PMA for 24 h, respectively. MUC5AC mucin gene expression and mucin protein production were measured by RT-PCR and ELISA. Oleanolic acid and ursolic acid were found to inhibit the production of MUC5AC mucin protein induced by EGF and PMA, and both compounds also inhibited the expression of MUC5AC mucin gene induced by EGF and PMA. These results suggest that oleanolic acid and ursolic acid can regulate mucin gene expression, and production of mucin protein, by directly acting on airway epithelial cells.
authors: Department of Otorhinolaryngology, Sanggye Paik Hospital, College of Medicine, Inje University, Seoul, Korea.
authors: Kyoungrai
authors: K
authors: Cho
authors: Hyun Jae
authors: HJ
authors: Lee
authors: Su Yel
authors: SY
authors: Lee
authors: Hoonyoung
authors: H
authors: Woo
authors: Mi Nam
authors: MN
authors: Lee
authors: Jeong Ho
authors: JH
authors: Seok
authors: Choong Jae
authors: CJ
authors: Lee
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D029768:Cornus / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D004815:Epidermal Growth Factor / Q000494:pharmacology; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D055271:Mucin 5AC / Q000096:biosynthesis / Q000187:drug effects* / Q000235:genetics; D009828:Oleanolic Acid / Q000494:pharmacology*; D010703:Phorbol Esters / Q000494:pharmacology; D012313:RNA / Q000235:genetics; D020545:Respiratory Mucosa / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014315:Triterpenes / Q000494:pharmacology*; D000097245:Ursolic Acid
chemical_list: D000890:Anti-Infective Agents; D004815:Epidermal Growth Factor; D055271:Mucin 5AC; D009828:Oleanolic Acid; D010703:Phorbol Esters; D012313:RNA; D014315:Triterpenes; D000097245:Ursolic Acid