title: The cellular factors Vps18 and Mon2 are required for efficient production of infectious HIV-1 particles.
abstract: Like all viruses, HIV-1 requires cellular host factors for replication. The mechanisms for production of progeny virions involving these host factors, however, are not fully understood. To better understand these mechanisms, we used a yeast (Saccharomyces cerevisiae) genetic screen to identify mutant strains in which HIV-1 Gag targeting to the plasma membrane was aberrant. Of the 917 mutants identified, we selected 14 mutants whose missing genes had single orthologous counterparts in human and tested them for Gag-induced viruslike particle (VLP) release in yeast cells. We found that the Vps18 and Mon2 proteins were important for HIV-1 Gag-induced VLP release in yeast. In eukaryote cells, these host proteins are highly conserved and function in protein trafficking. Depletion of hVps18 or hMon2 reduced the efficient production of infectious HIV-1 virions in human cells. Our data suggest that these cellular factors play an important role in the efficient production of infectious HIV-1 virion particles.
authors: Division of Virology, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo 108-8639, Japan.
authors: Yuriko
authors: Y
authors: Tomita
authors: Takeshi
authors: T
authors: Noda
authors: Ken
authors: K
authors: Fujii
authors: Tokiko
authors: T
authors: Watanabe
authors: Yuko
authors: Y
authors: Morikawa
authors: Yoshihiro
authors: Y
authors: Kawaoka
mesh_terms: D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D015497:HIV-1 / Q000254:growth & development* / Q000472:pathogenicity*; D054884:Host-Pathogen Interactions; D006801:Humans; D021381:Protein Transport; D006180:Proton-Translocating ATPases / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D033921:Vesicular Transport Proteins / Q000378:metabolism*; D022701:Virosomes / Q000378:metabolism; D014779:Virus Replication; D054301:gag Gene Products, Human Immunodeficiency Virus / Q000235:genetics / Q000378:metabolism
chemical_list: C529510:MON2 protein, human; C424437:VPS18 protein, human; D033921:Vesicular Transport Proteins; D022701:Virosomes; D054301:gag Gene Products, Human Immunodeficiency Virus; D006180:Proton-Translocating ATPases