title: Rac-1 GTPase controls the capacity of human leukaemic lymphoblasts to migrate on fibronectin in response to SDF-1α (CXCL12).
abstract: Acute lymphoblastic leukaemia (ALL) is characterized by malignant cell infiltration of bone marrow, requiring chemotactic response to SDF-1α. Using time-lapse video, we measured the velocity of ALL cells on fibronectin, and found that SDF-1α increased their migration activity for 2 h, but had no effect after 4h, following internalization of its receptor CXCR4. Transfection of ALL cells with dominant-negative Rac1 mutant significantly prolonged their chemotactic response to SDF-1α, and this effect was associated with an alteration of CXCR4 internalization. These data suggest a regulatory role for Rac1 in the chemotactic response of ALL cells to SDF-1α via receptor processing.
authors: Laboratoire MERCI (EA3829), Rouen University, and Department of Haematology, Rouen University Hospital, Rouen, France.
authors: Manuel
authors: M
authors: Freret
authors: Flore
authors: F
authors: Gouel
authors: Catherine
authors: C
authors: Buquet
authors: Elisabeth
authors: E
authors: Legrand
authors: Jean-Pierre
authors: JP
authors: Vannier
authors: Marc
authors: M
authors: Vasse
authors: Isabelle
authors: I
authors: Dubus
mesh_terms: D002465:Cell Movement; D054377:Chemokine CXCL12 / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism*; D005434:Flow Cytometry; D006801:Humans; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000378:metabolism* / Q000473:pathology*; D014407:Tumor Cells, Cultured; D020830:rac1 GTP-Binding Protein / Q000378:metabolism*
chemical_list: C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; D005353:Fibronectins; C107953:RAC1 protein, human; D020830:rac1 GTP-Binding Protein