title: Control of pelvic girdle development by genes of the Pbx family and Emx2.
abstract: Genes expressed in the somatopleuric mesoderm, the embryonic domain giving rise to the vertebrate pelvis, appear important for pelvic girdle formation. Among such genes, Pbx family members and Emx2 were found to genetically interact in hindlimb and pectoral girdle formation. Here, we generated compound mutant embryos carrying combinations of mutated alleles for Pbx1, Pbx2, and Pbx3, as well as Pbx1 and Emx2, to examine potential genetic interactions during pelvic development. Indeed, Pbx genes share overlapping functions and Pbx1 and Emx2 genetically interact in pelvic formation. We show that, in compound Pbx1;Pbx2 and Pbx1;Emx2 mutants, pelvic mesenchymal condensation is markedly perturbed, indicative of an upstream control by these homeoproteins. We establish that expression of Tbx15, Prrx1, and Pax1, among other genes involved in the specification and development of select pelvic structures, is altered in our compound mutants. Lastly, we identify potential Pbx1-Emx2-regulated enhancers for Tbx15, Prrx1, and Pax1, using bioinformatics analyses.
authors: Department of Cell and Developmental Biology, Weill Medical College of Cornell University, New York, New York, USA.
authors: Terence D
authors: TD
authors: Capellini
authors: Karen
authors: K
authors: Handschuh
authors: Laura
authors: L
authors: Quintana
authors: Elisabetta
authors: E
authors: Ferretti
authors: Giuseppina
authors: G
authors: Di Giacomo
authors: Sebastian
authors: S
authors: Fantini
authors: Giulia
authors: G
authors: Vaccari
authors: Shoa L
authors: SL
authors: Clarke
authors: Aaron M
authors: AM
authors: Wenger
authors: Gill
authors: G
authors: Bejerano
authors: James
authors: J
authors: Sharpe
authors: Vincenzo
authors: V
authors: Zappavigna
authors: Licia
authors: L
authors: Selleri
mesh_terms: D000818:Animals; D019295:Computational Biology; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000235:genetics / Q000502:physiology; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D051761:Paired Box Transcription Factors / Q000235:genetics / Q000378:metabolism; D010388:Pelvis / Q000196:embryology*; D000075062:Pre-B-Cell Leukemia Transcription Factor 1; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism; D020825:T-Box Domain Proteins / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D000099260:Empty Spiracles Homeobox Proteins
chemical_list: D018398:Homeodomain Proteins; D051761:Paired Box Transcription Factors; D000075062:Pre-B-Cell Leukemia Transcription Factor 1; D011518:Proto-Oncogene Proteins; D020825:T-Box Domain Proteins; D014157:Transcription Factors; D000099260:Empty Spiracles Homeobox Proteins; C481797:Pbx1 protein, mouse; C490382:Pbx2 protein, mouse; C489936:Prrx1 protein, mouse; C114093:TBX15 protein, mouse; C070835:PAX1 transcription factor; C090466:proto-oncogene protein Pbx3