title: Analysis of sonolytic degradation products of azo dye Orange G using liquid chromatography-diode array detection-mass spectrometry.
abstract: In this paper, seven new sonolytic degradation products of Orange G were found and identified using powerful analytical techniques such as liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS), tandem mass spectrometry (MS/MS), and liquid chromatography with diode-array detection (LC-DAD). Each technique provided complementary information for the degradation products identification. In order to resolve the MS and MS/MS spectra obtained, the separation conditions were optimized. Among them, Orange G was unambiguously identified based on its abundant [M-H](-) ion, [M+H](+) ion, ultra-violet and visible spectra, retention time, and tandem mass spectrometric analysis compared with an authentic standard. The seven new degradation products were tentatively identified based on ultra-violet and visible spectra, [M-H](-) ions, and tandem mass spectrometry. The neutral losses of SO(2), SO(3), N(2) and H(2)O for MS/MS spectra which appear to be characteristic of the negative ion mode were observed. Based on this by-product identification, a possible multi-step degradation scheme is proposed. The analysis results of degradation products reveal that the degradation mechanism proceeds via reductive cleavage of the azo linkage, as well as intermolecular dehydration and desulfonation due to the powerful oxidizing hydroxyl radicals as well as hydrogen radical.
authors: College of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou, China.
authors: Meiqiang
authors: M
authors: Cai
authors: Micong
authors: M
authors: Jin
authors: Linda K
authors: LK
authors: Weavers
mesh_terms: D001391:Azo Compounds / Q000737:chemistry* / Q000528:radiation effects*; D002853:Chromatography, Liquid / Q000379:methods*; D004396:Coloring Agents / Q000737:chemistry / Q000528:radiation effects; D013058:Mass Spectrometry / Q000379:methods*; D011829:Radiation Dosage; D012666:Semiconductors; D013010:Sonication / Q000379:methods*
chemical_list: D001391:Azo Compounds; D004396:Coloring Agents; C008710:Orange G