title: Neriifolin from seeds of Cerbera manghas L. induces cell cycle arrest and apoptosis in human hepatocellular carcinoma HepG2 cells.
abstract: The effects of neriifolin, a cardiac glycoside from Cerbera manghas L. on various end-points of oncologic relevance (cell growth, cell cycle regulation, and apoptosis in HepG2 cells) were investigated. Neriifolin reduced viability of HepG2 cells, induced S and G2/M phase arrests of the cell cycle, and stimulated apoptosis of HepG2 cells. Stimulation of HepG2 cells with neriifolin induced activation of caspase-3, -8, and -9, and up-regulated expression of Fas and FasL proteins. Based on these results, neriifolin could be considered a candidate for the treatment of hepatocellular carcinoma.
authors: Department of Biochemistry and Molecular Biology, Second Military Medical University, Shanghai, People's Republic of China.
authors: Qian
authors: Q
authors: Zhao
authors: Yuewei
authors: Y
authors: Guo
authors: Bo
authors: B
authors: Feng
authors: Liang
authors: L
authors: Li
authors: Caiguo
authors: C
authors: Huang
authors: Binghua
authors: B
authors: Jiao
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D029062:Apocynaceae / Q000737:chemistry*; D017209:Apoptosis / Q000187:drug effects*; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy* / Q000378:metabolism; D002298:Cardenolides / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D020169:Caspases / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects*; D053222:Fas Ligand Protein / Q000378:metabolism; D053200:Fas-Associated Death Domain Protein / Q000378:metabolism; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D012639:Seeds; D015854:Up-Regulation
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D002298:Cardenolides; D053222:Fas Ligand Protein; D053200:Fas-Associated Death Domain Protein; D010936:Plant Extracts; C040684:neriifolin; D020169:Caspases