title: A PQL (protein quantity loci) analysis of mature pea seed proteins identifies loci determining seed protein composition.
abstract: Legume seeds are a major source of dietary proteins for humans and animals. Deciphering the genetic control of their accumulation is thus of primary significance towards their improvement. At first, we analysed the genetic variability of the pea seed proteome of three genotypes over 3 years of cultivation. This revealed that seed protein composition variability was under predominant genetic control, with as much as 60% of the spots varying quantitatively among the three genotypes. Then, by combining proteomic and quantitative trait loci (QTL) mapping approaches, we uncovered the genetic architecture of seed proteome variability. Protein quantity loci (PQL) were searched for 525 spots detected on 2-D gels obtained for 157 recombinant inbred lines. Most protein quantity loci mapped in clusters, suggesting that the accumulation of the major storage protein families was under the control of a limited number of loci. While convicilin accumulation was mainly under the control of cis-regulatory regions, vicilins and legumins were controlled by both cis- and trans-regulatory regions. Some loci controlled both seed protein composition and protein content and a locus on LGIIa appears to be a major regulator of protein composition and of protein in vitro digestibility.
authors: INRA, UMR102, Dijon, France.
authors: Michael
authors: M
authors: Bourgeois
authors: Françoise
authors: F
authors: Jacquin
authors: Florence
authors: F
authors: Cassecuelle
authors: Vincent
authors: V
authors: Savois
authors: Maya
authors: M
authors: Belghazi
authors: Grégoire
authors: G
authors: Aubert
authors: Laurence
authors: L
authors: Quillien
authors: Myriam
authors: M
authors: Huart
authors: Pascal
authors: P
authors: Marget
authors: Judith
authors: J
authors: Burstin
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002853:Chromatography, Liquid; D002874:Chromosome Mapping; D004044:Dietary Proteins / Q000032:analysis / Q000378:metabolism; D017753:Ecosystem; D015180:Electrophoresis, Gel, Two-Dimensional; D018506:Gene Expression Regulation, Plant; D005838:Genotype; D006801:Humans; D007178:Inbreeding; D018532:Pisum sativum / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D010940:Plant Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D025341:Principal Component Analysis; D040901:Proteomics / Q000379:methods*; D040641:Quantitative Trait Loci; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics; D055314:Seed Storage Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000235:genetics / Q000378:metabolism*; D019265:Spectroscopy, Near-Infrared; D000097793:Legumins
chemical_list: D004044:Dietary Proteins; D010940:Plant Proteins; D055314:Seed Storage Proteins; D000097793:Legumins; C029781:CVC protein, Pisum sativum; C015575:vicilin protein, plant