title: [Effects of three root-end filling materials on human periodontal ligament cells in an in vitro wound model].
abstract: OBJECTIVE
  To compare the effects of mineral trioxide aggregate (MTA), amalgam and intermediate restorative material (IRM) on human periodontal ligament cells (HPDLC) using an in vitro wound healing model.
  
  
  METHODS
  Wound was created by making a 3mm in width incision in the cell monolayer across the length of tissue culture plate, then the cell monolayer was treated with media containing MTA, IRM, and amalgam extracts, respectively. Medium supplemented with fetal bovine serum (10%FBS) served as positive control, and that with 0.1% FBS as negative control. The cells were incubated for 2, 6 and 9 days, and at each time point, the levels of both proliferation and cellular wound filling were measured across the width of the wound. SPSS 11.5 software package was used for analyze.
  
  
  RESULTS
  There was little cellular repopulation of the wound on the day 2. On the day 6 and day 9, the order of response was MTA > amalgam > IRM (P < 0.05). For the cell proliferative activity, the order was MTA > amalgam approximately equal to IRM on the day 2.
  
  
  CONCLUSIONS
  This study suggests that the degree of cytotoxic effect in ascending order is MTA, amalgam and IRM.
authors: Department of Pediatric Dentistry, School and Hospital of Stomatology, Wenzhou Medical College, Wenzhou 325027, Zhejiang Province, China. lealyne@163.com
authors: Ling
authors: L
authors: Li
authors: Qian
authors: Q
authors: Wang
mesh_terms: D017607:Aluminum Compounds; D017610:Calcium Compounds; D003723:Dental Amalgam; D004338:Drug Combinations; D005347:Fibroblasts; D006801:Humans; D066298:In Vitro Techniques; D010087:Oxides; D010513:Periodontal Ligament; D012387:Root Canal Filling Materials; D012389:Root Canal Obturation; D017640:Silicates
chemical_list: D017607:Aluminum Compounds; D017610:Calcium Compounds; D004338:Drug Combinations; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; C086631:mineral trioxide aggregate; D003723:Dental Amalgam