title: Genetic diversity of HPV-16 E6, E7, and L1 genes in women with cervical lesions in Liaoning Province, China.
abstract: BACKGROUND
  High-risk human papillomaviruses (HPVs) play a cardinal role in the etiology of cervical cancer. The most prevalent type, HPV-16, shows intratypic sequence variants that are known to differ in oncogenic potential and geographic distribution. Intratype variations in oncogenic E6/E7 and capsid L1 proteins of HPV-16 are associated with risk of viral persistence and progression.
  
  
  METHODS
  This study was designed to analyze sequence variations in E6, E7, and L1 genes of HPV-16 in patients with cervical lesion to identify the most prevalent and novel HPV-16 variants in northern China.
  
  
  RESULTS
  Our results showed that HPV-16 variants with respect to E6 and E7 were high prevalence of the Asian lineage: 48.3% and 51.4%, respectively. Sequences of the E6 gene revealed 4 amino acid changes of variants D25E and L83V, with 48.3% (69/143) and 11.2% (16/143), respectively, and variants H78Y and E113D in this study. The results also showed the prevalence of 4 hot spots of E7 nucleotide variations leading to N29H, N29S, and 2 silent variations, nucleotide G666A and nucleotide T846C, with 4.2% (6/142), 43% (61/142), 32.4% (46/142), and 43% (61/142), respectively. The following L1 variations were found in this study: L103F, P104K, P104Y, P104S, D105G, P106S, N108P, F109V, C172S, H228D, and T292A. It was also found that 448S was inserted and 465D was deleted in the L1 amino acid sequences of all the samples. No significant relationship between HPV-16 variants and high-grade lesions was found.
  
  
  CONCLUSIONS
  The study provides some new data on the genetic diversity of HPV-16, which may help to understand the oncogenic potential of the virus and design the diagnosis reagents and vaccine of HPV in China. Furthermore, in-depth studies are needed to determine the clinical and biological effects of these variants.
authors: Virus Laboratory, The Affiliated Shengjing Hospital, China Medical University, Shenyang, Liaoning, China.
authors: Zhengrong
authors: Z
authors: Sun
authors: Gaowei
authors: G
authors: Ren
authors: Xin
authors: X
authors: Cui
authors: Weiqiang
authors: W
authors: Zhou
authors: Chao
authors: C
authors: Liu
authors: Qiang
authors: Q
authors: Ruan
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D036022:Capsid Proteins / Q000235:genetics*; D002681:China; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D014644:Genetic Variation; D006801:Humans; D008875:Middle Aged; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D050725:Papillomavirus E7 Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011379:Prognosis; D012097:Repressor Proteins / Q000235:genetics*; D002577:Uterine Cervical Diseases / Q000235:genetics*; D055815:Young Adult
chemical_list: D036022:Capsid Proteins; D004279:DNA, Viral; C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D012097:Repressor Proteins; C059731:oncogene protein E7, Human papillomavirus type 16; C063317:L1 protein, Human papillomavirus type 16