title: A quantum-dot based protein module for in vivo monitoring of protease activity through fluorescence resonance energy transfer.
abstract: Here, we present a new generation of nanoscale probes for in vivo monitoring of protease activity by fluorescence resonance energy transfer (FRET). The approach is based on a genetically programmable protein module carrying a fluorescently labeled, protease-specific sequence that can self-assemble onto quantum dots. The protein module was used for real-time detection of human immunodeficiency virus type-1 protease (HIV-1 Pr) activity as well as quantitative assessment of inhibitor efficiency.
authors: Department of Chemical and Environmental Engineering, University of California, Riverside, California 92521, USA.
authors: Payal
authors: P
authors: Biswas
authors: Lakshmi N
authors: LN
authors: Cella
authors: Seung Hyun
authors: SH
authors: Kang
authors: Ashok
authors: A
authors: Mulchandani
authors: Marylynn V
authors: MV
authors: Yates
authors: Wilfred
authors: W
authors: Chen
mesh_terms: D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D016333:HIV Protease / Q000737:chemistry* / Q000378:metabolism; D015497:HIV-1 / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D045663:Quantum Dots
chemical_list: D011506:Proteins; D016333:HIV Protease; C514803:p16 protease, Human immunodeficiency virus 1