title: Probing the roles of LRR RLK genes in Arabidopsis thaliana roots using a custom T-DNA insertion set.
abstract: Leucine-rich repeat receptor-like protein kinases (LRR RLKs) represent the largest group of Arabidopsis RLKs with approximately 235 members. A minority of these LRR RLKs have been assigned to diverse roles in development, pathogen resistance and hormone perception. Using a reverse genetics approach, a collection of homozygous T-DNA insertion lines for 69 root expressed LRR RLK genes was screened for root developmental defects and altered response after exposure to environmental, hormonal/chemical and abiotic stress. The obtained data demonstrate that LRR RLKs play a role in a wide variety of signal transduction pathways related to hormone and abiotic stress responses. The described collection of T-DNA insertion mutants provides a valuable tool for future research into the function of LRR RLK genes.
authors: Faculty of Science, Department of Biology, Section Molecular Genetics, Utrecht University, Padualaan 8, Utrecht, The Netherlands.
authors: Colette A
authors: CA
authors: ten Hove
authors: Zoltán
authors: Z
authors: Bochdanovits
authors: Vera M A
authors: VM
authors: Jansweijer
authors: Fenne G
authors: FG
authors: Koning
authors: Lidija
authors: L
authors: Berke
authors: Gabino F
authors: GF
authors: Sanchez-Perez
authors: Ben
authors: B
authors: Scheres
authors: Renze
authors: R
authors: Heidstra
mesh_terms: D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects / Q000528:radiation effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000528:radiation effects; D007210:Indoleacetic Acids / Q000494:pharmacology; D000090263:Leucine-Rich Repeat Proteins; D008027:Light; D008353:Mannitol / Q000494:pharmacology; D016254:Mutagenesis, Insertional; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000494:pharmacology; D018517:Plant Roots / Q000201:enzymology* / Q000235:genetics; D011494:Protein Kinases / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D011506:Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects / Q000528:radiation effects; D012965:Sodium Chloride / Q000494:pharmacology
chemical_list: D029681:Arabidopsis Proteins; D004269:DNA, Bacterial; D007210:Indoleacetic Acids; D000090263:Leucine-Rich Repeat Proteins; D010937:Plant Growth Regulators; D011506:Proteins; C036483:T-DNA; D008353:Mannitol; D012965:Sodium Chloride; C030737:indoleacetic acid; D011494:Protein Kinases