title: Pharmacological and toxicological assessment of a potential GnRH vaccine in young-adult male pigs.
abstract: Active immunization against gonadotrophin-releasing hormone (GnRH) is successfully applied to prevent boar taint in pork. In men, GnRH immunization could be an alternative to hormone therapy in patients with prostate cancer. In this study, a new GnRH vaccine formulation (a modified GnRH peptide conjugate formulated with CoVaccine adjuvant) was investigated for its pharmacological efficacy and safety in young-adult male pigs. Immunization resulted in castrate-like plasma testosterone levels in all treated pigs from week 8 until the end of the study, 30 weeks after the first immunization. Testosterone depletion retarded testes growth, reduced the relative weight of the testes and accessory sex organs, and reduced sperm counts and motility. There was no clinically relevant toxicity. Typical vaccination-related adverse reactions, such as swelling at the injection site and fever, were considered acceptable. We conclude that this GnRH vaccine efficiently and rapidly reduced serum testosterone levels, without inducing chronic toxic effects, and therefore could be applicable in both veterinary and human medicine.
authors: Pepscan Therapeutics, Zuidersluisweg 2, 8243 RC Lelystad, The Netherlands.
authors: J A
authors: JA
authors: Turkstra
authors: F J
authors: FJ
authors: van der Staay
authors: N
authors: N
authors: Stockhofe-Zurwieden
authors: H
authors: H
authors: Woelders
authors: R H
authors: RH
authors: Meloen
authors: T
authors: T
authors: Schuurman
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D001831:Body Temperature; D064420:Drug-Related Side Effects and Adverse Reactions / Q000662:veterinary; D007987:Gonadotropin-Releasing Hormone / Q000276:immunology / Q000494:pharmacology*; D006062:Gonadotropins / Q000097:blood; D007114:Immunization / Q000009:adverse effects / Q000662:veterinary; D008297:Male; D009929:Organ Size; D012741:Sexual Maturation; D013076:Sperm Count / Q000662:veterinary; D013081:Sperm Motility; D034421:Sus scrofa / Q000502:physiology*; D013737:Testis / Q000502:physiology; D013739:Testosterone / Q000097:blood; D014508:Urea / Q000097:blood; D014612:Vaccines / Q000009:adverse effects / Q000494:pharmacology*
chemical_list: D000276:Adjuvants, Immunologic; D006062:Gonadotropins; D014612:Vaccines; D007987:Gonadotropin-Releasing Hormone; D013739:Testosterone; D014508:Urea