title: Engineered tendon with decellularized xenotendon slices and bone marrow stromal cells: an in vivo animal study.
abstract: The purpose of this study was to investigate an engineered composite of multilayer acellular tendon slices seeded with bone marrow stromal cells (BMSCs) as a possible solution for tendon reconstruction. BMSCs were harvested from 15 rabbits and infraspinatus tendons were harvested from 17 dogs. The decellularized tendons were sectioned in longitudinal slices with a thickness of 50 µm. The BMSCs were seeded on the slices and then the slices were bundled into one composite. The composite was implanted into a rabbit patellar tendon defect. Tendon slices without BMSCs were implanted into the contralateral patellar tendon as a control. The composites were evaluated by histology and qRT-PCR. The viability of BMSCs was assessed using a fluorescent marker. Histology showed viable cells between the collagen fibres on the cell-seeded side. Analysis by qRT-PCR showed higher tenomodulin, collagen type III, MMP3 and MMP13 expressions and lower collagen type I expression in the cell-seeded composite than in the tendon slices without BMSCs. We conclude that BMSCs can survive in a multilayer composite, express a tendon phenotype and enhance the metabolism of tendon in vivo. This in vivo study suggests a potential utility of this composite in tendon reconstruction.
authors: Orthopedic Biomechanics Laboratory, Mayo Clinic, Rochester, MN 55905, USA.
authors: Hiromichi
authors: H
authors: Omae
authors: Yu Long
authors: YL
authors: Sun
authors: Kai-Nan
authors: KN
authors: An
authors: Peter C
authors: PC
authors: Amadio
authors: Chunfeng
authors: C
authors: Zhao
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D016026:Bone Marrow Transplantation; D058948:Cell Tracking; D004285:Dogs; D005786:Gene Expression Regulation; D011817:Rabbits; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism / Q000637:transplantation; D013710:Tendons / Q000166:cytology* / Q000637:transplantation*; D023822:Tissue Engineering / Q000379:methods*