title: Application of the logic of cysteine-free native chemical ligation to the synthesis of Human Parathyroid Hormone (hPTH).
abstract: The power of chemical synthesis of large cysteine-free polypeptides has been significantly enhanced through the use of nonproteogenic constructs which bear strategically placed thiol groups, enabling native chemical ligation. Central to these much expanded capabilities is the specific, radical-induced, metal-free dethiolation, which can be accomplished in aqueous medium.
authors: Bio-Organic Chemistry Laboratory, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10065, USA.
authors: Shiying
authors: S
authors: Shang
authors: Zhongping
authors: Z
authors: Tan
authors: Samuel J
authors: SJ
authors: Danishefsky
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D002942:Circular Dichroism; D003545:Cysteine / Q000737:chemistry*; D006801:Humans; D008969:Molecular Sequence Data; D010281:Parathyroid Hormone / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D010281:Parathyroid Hormone; D003545:Cysteine