title: [Effect of exogenous Ca2+, ALA, SA and Spd on seed germination and physiological characteristics of Perilla frutescens seedlings under NaCl stress].
abstract: OBJECTIVE
  To find a method for improving the salt resistance of seeds and seedlings for Perilla Frutescens under NaCl stress, seed germination and physiological characteristics of P. frutescens seedlings were studied.
  
  
  METHODS
  Several physiological indexes of P. frutescens seeds treated with different concentrations of Ca2+, 5-aminolevulinic acid (ALA), salicylic acid (SA) and spermidine (Spd) under NaCl stress like the germination vigor, germination rate, germination index and vigor index were measured. And other indexes like the biomass of the seedlings, the content of malondialdehyde (MDA) in leaves, the activities of superoxide (SOD), peroxidase (POD) and catalase (CAT) were also measured.
  
  
  RESULTS
  The germination of P. frutescens seeds under NaCl stress (100 mmol x L(-1)) was inhibited obviously. But after the treatment with Ca2+, ALA , SA and Spd, all germination indexes were increased. Ca2+ (10 mmol x L(-1)), ALA (100 mg x L(-1)), SA (50 mg x L(-1)) and Spd (0.25 mmol x L(-1)) could obviously alleviate the damage of salt stress to the seeds of P. frutescens. ALA (100 mg x L(-1)) significantly increased all indexes. The germination vigor was 65.3%, the germination rate was 89.7%, the germination index and vigor index were 15.2 and 0.1238, respectively. All treatments decreased the content of MDA in leaves. The activities of three enzymes including SOD, POD and CAT were all increased. ALA (100 mg x L(-1)) had the enzymes activity reach the maximum with 0.72, 6, 82 and 5.64 U x mg(-1), respectively.
  
  
  CONCLUSIONS
  Ca2+ ALA , SA and Spd with appropriate concentration could significantly alleviate the damages to the seeds and seedlings of P. frutescens under NaCl stress and promote the salt resistance of the seeds and seedlings.
authors: School of Life Sciences, Southwest University, Key Laboratory of Eco-environments of Three Gorges Reservoir Region of Ministry of Education, Chongqing Key Laboratory of Plant Ecology and Resources Research for Three Gorges Reservoir Region, Chongqing 400715, China. chunpingzhang520@163.com
authors: Chunping
authors: C
authors: Zhang
authors: Ping
authors: P
authors: He
authors: Zeli
authors: Z
authors: Yu
authors: Dandan
authors: D
authors: Du
authors: Pinxiang
authors: P
authors: Wei
mesh_terms: D000622:Aminolevulinic Acid / Q000494:pharmacology*; D002118:Calcium / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D018525:Germination / Q000187:drug effects*; D008315:Malondialdehyde / Q000378:metabolism; D031344:Perilla frutescens / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism / Q000502:physiology*; D009195:Peroxidase / Q000378:metabolism; D020156:Salicylic Acid / Q000494:pharmacology*; D036226:Seedlings / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism / Q000502:physiology; D012965:Sodium Chloride / Q000494:pharmacology; D013095:Spermidine / Q000494:pharmacology*; D013312:Stress, Physiological / Q000187:drug effects*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D012965:Sodium Chloride; D008315:Malondialdehyde; D000622:Aminolevulinic Acid; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase; D020156:Salicylic Acid; D002118:Calcium; D013095:Spermidine