title: Positive selection in the hemagglutinin-neuraminidase gene of Newcastle disease virus and its effect on vaccine efficacy.
abstract: BACKGROUND
  To investigate the relationship between the selective pressure and the sequence variation of the hemagglutinin-neuraminidase (HN) protein, we performed the positive selection analysis by estimating the ratio of non-synonymous to synonymous substitutions with 132 complete HN gene sequences of Newcastle disease viruses (NDVs) isolated in China.
  
  
  RESULTS
  The PAML software applying a maximum likelihood method was used for the analysis and three sites (residues 266, 347 and 540) in the HN protein were identified as being under positive selection. Codon 347 was located exactly in a recognized antigenic determinant (residues 345-353) and codon 266 in a predicted linear B-cell epitope. Substitutions at codon 540 contributed to the N-linked glycosylation potential of residue 538. To further evaluate the effect of positively selected sites on the vaccine efficacy, we constructed two recombinant fowlpox viruses rFPV-JS6HN and rFPV-LaSHN, expressing the HN proteins from a genotype VII field isolate Go/JS6/05 (with A266, K347 and A540) and vaccine strain La Sota (with V266, E347 and T540), respectively. Two groups of SPF chickens, 18 each, were vaccinated with the two recombinant fowlpox viruses and challenged by Go/JS6/05 at 3 weeks post-immunization. The results showed that rFPV-JS6HN could elicit more effective immunity against the prevalent virus infection than rFPV-LaSHN in terms of reducing virus shedding.
  
  
  CONCLUSIONS
  The analysis of positively selected codons and their effect on the vaccine efficacy indicated that the selective pressure on the HN protein can induce antigenic variation, and new vaccine to control the current ND epidemics should be developed.
authors: Animal Infectious Disease Laboratory, School of Veterinary Medicine, Yangzhou University, Yangzhou 225009, PR China.
authors: Min
authors: M
authors: Gu
authors: Wujie
authors: W
authors: Liu
authors: Lijun
authors: L
authors: Xu
authors: Yongzhong
authors: Y
authors: Cao
authors: Chunfeng
authors: C
authors: Yao
authors: Shunlin
authors: S
authors: Hu
authors: Xiufan
authors: X
authors: Liu
mesh_terms: D000818:Animals; D002642:Chick Embryo; D002645:Chickens; D010856:Columbidae; D015707:HN Protein / Q000008:administration & dosage / Q000235:genetics* / Q000276:immunology*; D008969:Molecular Sequence Data; D009521:Newcastle Disease / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D009522:Newcastle disease virus / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000276:immunology; D010802:Phylogeny; D011201:Poultry Diseases / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D012641:Selection, Genetic; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
chemical_list: D015707:HN Protein; D014765:Viral Vaccines