title: Comparative study of clinical pulmonary surfactants using atomic force microscopy.
abstract: Clinical pulmonary surfactant is routinely used to treat premature newborns with respiratory distress syndrome, and has shown great potential in alleviating a number of neonatal and adult respiratory diseases. Despite extensive study of chemical composition, surface activity, and clinical performance of various surfactant preparations, a direct comparison of surfactant films is still lacking. In this study, we use atomic force microscopy to characterize and compare four animal-derived clinical surfactants currently used throughout the world, i.e., Survanta, Curosurf, Infasurf and BLES. These modified-natural surfactants are further compared to dipalmitoyl phosphatidylcholine (DPPC), a synthetic model surfactant of DPPC:palmitoyl-oleoyl phosphatidylglycerol (POPG) (7:3), and endogenous bovine natural surfactant. Atomic force microscopy reveals significant differences in the lateral structure and molecular organization of these surfactant preparations. These differences are discussed in terms of DPPC and cholesterol contents. We conclude that all animal-derived clinical surfactants assume a similar structure of multilayers of fluid phospholipids closely attached to an interfacial monolayer enriched in DPPC, at physiologically relevant surface pressures. This study provides the first comprehensive survey of the lateral structure of clinical surfactants at various surface pressures. It may have clinical implications on future application and development of surfactant preparations.
authors: Department of Mechanical Engineering, University of Hawaii at Manoa, Honolulu, HI 96822, USA.
authors: Hong
authors: H
authors: Zhang
authors: Qihui
authors: Q
authors: Fan
authors: Yi E
authors: YE
authors: Wang
authors: Charles R
authors: CR
authors: Neal
authors: Yi Y
authors: YY
authors: Zuo
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry; D000818:Animals; D002417:Cattle; D006801:Humans; D018625:Microscopy, Atomic Force / Q000379:methods*; D008956:Models, Chemical; D010715:Phosphatidylglycerols / Q000737:chemistry; D011663:Pulmonary Surfactants / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D010715:Phosphatidylglycerols; D011663:Pulmonary Surfactants; D015060:1,2-Dipalmitoylphosphatidylcholine; C060037:1-palmitoyl-2-oleoylglycero-3-phosphoglycerol