title: Highly sensitive fluorescence detection system for microfluidic lab-on-a-chip.
abstract: We demonstrate a compact, low cost and practical fluorescence detection system for lab-on-a-chip applications. The system comprises a commercially available InGaN light emitting diode (501 nm) as light source, an organic or silicon photodiode detector, absorptive dye coated colour filters and linear and reflective polarisers. An injection moulded polystyrene microfluidic chip is used as the platform for fluorescence immunoassays for cardiac markers myoglobin and CK-MB. The optical limit of detection (LOD) is measured using a TransFluoSphere® suspension at 5.6 × 10(4) beads µl(-1) which can be equated to ∼3 nM fluorescein equivalent concentration. The LOD for the human plasma immunoassays is measured as 1.5 ng ml(-1) for both myoglobin and CK-MB.
authors: Molecular Vision Ltd. BioIncubator Unit, Bessemer Building, Imperial College London, London, SW7 2BP, UK.
authors: Gihan
authors: G
authors: Ryu
authors: Jingsong
authors: J
authors: Huang
authors: Oliver
authors: O
authors: Hofmann
authors: Claire A
authors: CA
authors: Walshe
authors: Jasmine Y Y
authors: JY
authors: Sze
authors: Gareth D
authors: GD
authors: McClean
authors: Alan
authors: A
authors: Mosley
authors: Simon J
authors: SJ
authors: Rattle
authors: John C
authors: JC
authors: deMello
authors: Andrew J
authors: AJ
authors: deMello
authors: Donal D C
authors: DD
authors: Bradley
mesh_terms: D015415:Biomarkers / Q000097:blood; D052279:Creatine Kinase, MB Form / Q000097:blood; D005455:Fluorescent Antibody Technique / Q000379:methods*; D006801:Humans; D056656:Lab-On-A-Chip Devices; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D009211:Myoglobin / Q000097:blood; D019095:Point-of-Care Systems; D012680:Sensitivity and Specificity
chemical_list: D015415:Biomarkers; D009211:Myoglobin; D052279:Creatine Kinase, MB Form