title: Metabolite profiling of pea roots in response to phosphate availability.
abstract: The arbuscular mycorrhizal (AM) symbiosis is a mutualistic association between soil fungi (Glomeromycota) and roots of most plant species. A recent study showed that high phosphate fertilization could inhibit mycorrhizal colonization at a very early stage, before hyphopodium formation. The authors proposed that inhibiting and/or stimulatory compounds might be present in roots grown under high phosphate or low phosphate, respectively. To further address this question, we performed metabolite profiling analyses of extracts of pea roots grown under low and high phosphate concentrations. Ultra high performance liquid chromatography (UHPLC) was coupled with high resolution (HR) mass spectrometry (Q-TOF) and multivariate statistical analysis. This allowed the detection of 34 ions discriminating the two conditions. A majority (28 ions) were more abundant in roots grown under low phosphate concentration, and among them four were specific of this condition. The results suggest that the regulation of AM symbiosis by phosphate may involve the synthesis or accumulation of stimulatory compounds in roots grown under low phosphate.
authors: Université de Toulouse, UPS, UMR 5546, Laboratoire de Recherche en Sciences Végétales (LRSV), Toulouse, France.
authors: Jérôme
authors: J
authors: Laparre
authors: Coline
authors: C
authors: Balzergue
authors: Soizic
authors: S
authors: Rochange
authors: Pascal
authors: P
authors: Ludwiczak
authors: Fabien
authors: F
authors: Letisse
authors: Jean Charles
authors: JC
authors: Portais
authors: Guillaume
authors: G
authors: Bécard
authors: Virginie
authors: V
authors: Puech-Pages
mesh_terms: D002851:Chromatography, High Pressure Liquid; D016002:Discriminant Analysis; D013058:Mass Spectrometry; D055432:Metabolomics / Q000379:methods*; D018532:Pisum sativum / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism*; D010710:Phosphates / Q000494:pharmacology*; D010936:Plant Extracts / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects* / Q000378:metabolism*; D025341:Principal Component Analysis
chemical_list: D010710:Phosphates; D010936:Plant Extracts