title: A modified CDC biofilm reactor to produce mature biofilms on the surface of peek membranes for an in vivo animal model application.
abstract: Biofilm-related infections have become a major clinical concern. Typically, animal models that involve inoculation with planktonic bacteria have been used to create positive infection signals and examine antimicrobial strategies for eradicating or preventing biofilm-related infection. However, it is estimated that 99.9% of bacteria in nature dwell in established biofilms. As such, open wounds have significant potential to become contaminated with bacteria that reside in a well-established biofilm. In this study, a modified CDC biofilm reactor was developed to repeatably grow mature biofilms of Staphylococcus aureus on the surface of polyetheretherketone (PEEK) membranes for inoculation in a future animal model of orthopaedic implant biofilm-related infection. Results indicated that uniform, mature biofilms repeatably grew on the surface of the PEEK membranes.
authors: Department of Bioengineering, University of Utah, Salt Lake City, UT, USA. dustin.williams@utah.edu
authors: Dustin L
authors: DL
authors: Williams
authors: Kassie L
authors: KL
authors: Woodbury
authors: Bryan S
authors: BS
authors: Haymond
authors: Albert E
authors: AE
authors: Parker
authors: Roy D
authors: RD
authors: Bloebaum
mesh_terms: D000818:Animals; D018441:Biofilms; D019149:Bioreactors / Q000382:microbiology*; D006801:Humans; D007718:Knee Injuries / Q000382:microbiology* / Q000601:surgery; D008567:Membranes, Artificial; D055624:Methicillin-Resistant Staphylococcus aureus / Q000502:physiology*; D023421:Models, Animal; D016459:Prosthesis-Related Infections / Q000382:microbiology*
chemical_list: D008567:Membranes, Artificial