title: Cell uptake and trafficking behavior of non-covalent, coiled-coil based polymer-drug conjugates.
abstract: This paper reports on the cell uptake and trafficking properties of a series of non-covalent polymer-drug conjugates. These nanomedicines are composed of a poly(N-(2-hydroxypropyl)methacrylamide) backbone functionalized with multiple copies of a drug. The drug moieties are attached to the polymer via a non-covalent, so called coiled coil motif, which is formed by heterodimerization of two complementary peptide strands, one of which is attached to the polymer carrier and the other to the drug. Cytotoxicity and FACS experiments, which were carried out with model anticancer drug or fluorophore conjugates, provided insight into the cell uptake and trafficking behavior of these conjugates.
authors: École Polytechnique Fédérale de Lausanne, Institut des Matériaux and Institut des Sciences et Ingénierie Chimiques, Laboratoire des Polymères, Bâtiment MXD, Station 12, CH-1015 Lausanne, Switzerland.
authors: Bojana
authors: B
authors: Apostolovic
authors: Samuel P E
authors: SP
authors: Deacon
authors: Ruth
authors: R
authors: Duncan
authors: Harm-Anton
authors: HA
authors: Klok
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000737:chemistry*; D045744:Cell Line, Tumor; D058948:Cell Tracking; D002942:Circular Dichroism; D019281:Dimerization; D004337:Drug Carriers / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D008727:Methotrexate / Q000737:chemistry; D051379:Mice; D050997:Nanomedicine; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry; D011109:Polymethacrylic Acids / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D005456:Fluorescent Dyes; D010455:Peptides; D011109:Polymethacrylic Acids; C011533:Duxon; D008727:Methotrexate