title: MicroRNA knock down by cholesterol-conjugated antisense oligos in mouse organ culture.
abstract: Here, we detail a protocol to design and introduce sequence-specific cholesterol-conjugated antisense oligonucleotides into mouse organ culture. We review design principles for "antagomirs", antisense oligos with a cholesterol-moiety modification at the 3', and present an optimized method to apply them onto 3D cultured embryonic pancreas. The method offers an approach to study the developmental functions of individual miRNAs and to evaluate miRNA targets, which is significantly faster and simpler than comparable genetics-based approaches.
authors: Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.
authors: Sharon
authors: S
authors: Kredo-Russo
authors: Eran
authors: E
authors: Hornstein
mesh_terms: D000818:Animals; D002784:Cholesterol; D055785:Gene Knockdown Techniques; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D016376:Oligonucleotides, Antisense / Q000235:genetics*; D009924:Organ Culture Techniques; D010179:Pancreas
chemical_list: D035683:MicroRNAs; D016376:Oligonucleotides, Antisense; D002784:Cholesterol