title: Biocompatibility of RealSeal, its primer and AH Plus implanted in subcutaneous connective tissue of rats.
abstract: OBJECTIVE
  This study tested rat connective tissue response to RealSeal, RealSeal primer or AH Plus after 7, 15, 30, 60 and 90 days of implantation.
  
  
  METHODS
  Thirty Wistar rats had subcutaneous sockets created on their back and received four implants each of polyethylene tubes containing one of the materials tested according to the groups: AH (AH Plus Sealer); RS (RealSeal Sealer); RP (RealSeal Primer); CG (control group - empty tube). After histological processing, sections were analyzed to identify the presence of neutrophils, lymphocytes and plasma cells, eosinophils, macrophages and giant cells, as well as fibrous capsule and abscesses, by an examiner using light microscope. Kruskal-Wallis and multiple-comparisons test were used for statistical analysis. Significance level was set at 5%.
  
  
  RESULTS
  Lymphoplasmacytic infiltrate scores significantly higher than those of the control group were observed at 14 and 60 days in AH group, and at 90 days in RS group (p<0.05). There were no differences in terms of presence of macrophages, giant cells, eosinophils, neutrophils or fibrosis. AH Plus group scored higher for abscesses at 7 days than after any other period (p=0.031). RP group scored higher for lymphoplasmacytic infiltrate at 14 days than at 90 days (p=0.04).
  
  
  CONCLUSIONS
  The main contribution of this study was to demonstrate that issues involved with tissue tolerance of a Resilon-containing sealer, RealSeal Sealer, cannot be attributed to its primer content.
authors: Department of Conservative Dentistry, Dental School, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil. fabiana.grecca@ufrgs.br
authors: Fabiana Soares
authors: FS
authors: Grecca
authors: Patrícia Maria Poli
authors: PM
authors: Kopper
authors: Régis Burmeister dos
authors: RB
authors: Santos
authors: Anna Christina
authors: AC
authors: Fossati
authors: Vinicius Coelho
authors: VC
authors: Carrard
authors: Gerson Arison Xavier
authors: GA
authors: Acasigua
authors: José Antônio Poli de
authors: JA
authors: Figueiredo
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D003188:Composite Resins / Q000009:adverse effects*; D004853:Epoxy Resins / Q000633:toxicity*; D008297:Male; D008422:Materials Testing; D023421:Models, Animal; D020959:Polyethylene; D051381:Rats; D017208:Rats, Wistar; D012387:Root Canal Filling Materials / Q000009:adverse effects*; D040521:Subcutaneous Tissue / Q000187:drug effects*; D013997:Time Factors
chemical_list: D001672:Biocompatible Materials; D003188:Composite Resins; D004853:Epoxy Resins; C511498:Real Seal composite resin; D012387:Root Canal Filling Materials; C534916:epoxy resin-based root canal sealer; D020959:Polyethylene