title: Differential expression of complement proteins in cerebrospinal fluid from active multiple sclerosis patients.
abstract: Multiple sclerosis (MS) is a demyelinating disease of the central nervous system with complex immunopathogenesis. Using the 2-D DIGE technology, we separate CSF proteins from patients with active MS and control subjects. Three of the seven differential proteins identified were related with complement system, and the network analysis of the differential proteins revealed complement activation involvement in active MS. Complement C4b (gamma chain) was confirmed elevated by performing western blotting analysis (P < 0.01). The present results are an independent quantitative proteomic measure in CSF from active MS patients. The differential expression of the complement C4b and related proteins in CSF provides potential biomarkers as well as evidence for the involvement of complement activation in the pathogenesis of MS disease.
authors: Institute of Biochemistry and Molecular Biology, School of Medicine, Shandong University, Jinan 250012, China.
authors: Yun
authors: Y
authors: Li
authors: Zhaoyu
authors: Z
authors: Qin
authors: Mingchong
authors: M
authors: Yang
authors: Yanjiang
authors: Y
authors: Qin
authors: Chengzhao
authors: C
authors: Lin
authors: Shilian
authors: S
authors: Liu
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000134:cerebrospinal fluid; D016022:Case-Control Studies; D003165:Complement System Proteins / Q000134:cerebrospinal fluid* / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000134:cerebrospinal fluid*; D025941:Protein Interaction Mapping
chemical_list: D015415:Biomarkers; D003165:Complement System Proteins