title: Btn3 is a negative regulator of Btn2-mediated endosomal protein trafficking and prion curing in yeast.
abstract: Yeast Btn2 facilitates the retrieval of specific proteins from late endosomes (LEs) to the Golgi, a process that may be adversely affected in Batten disease patients. We isolated the putative yeast orthologue of a human complex I deficiency gene, designated here as BTN3, as encoding a Btn2-interacting protein and negative regulator. First, yeast overexpressing BTN3 phenocopy the deletion of BTN2 and mislocalize certain trans-Golgi proteins, like Kex2 and Yif1, to the LE and vacuole, respectively. In contrast, the deletion of BTN3 results in a tighter pattern of protein localization to the Golgi. Second, BTN3 overexpression alters Btn2 localization from the IPOD compartment, which correlates with a sharp reduction in Btn2-mediated [URE3] prion curing. Third, Btn3 and the Snc1 v-SNARE compete for the same binding domain on Btn2, and this competition controls Btn2 localization and function. The inhibitory effects upon protein retrieval and prion curing suggest that Btn3 sequesters Btn2 away from its substrates, thus down-regulating protein trafficking and aggregation. Therefore Btn3 is a novel negative regulator of intracellular protein sorting, which may be of importance in the onset of complex I deficiency and Batten disease in humans.
authors: Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel.
authors: Vydehi
authors: V
authors: Kanneganti
authors: Rachel
authors: R
authors: Kama
authors: Jeffrey E
authors: JE
authors: Gerst
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport; D026905:Amino Acid Transport Systems / Q000378:metabolism*; D015536:Down-Regulation; D056827:Endosomal Sorting Complexes Required for Transport / Q000378:metabolism; D011992:Endosomes / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D006360:Heat-Shock Proteins / Q000378:metabolism; D009472:Neuronal Ceroid-Lipofuscinoses / Q000378:metabolism; D011328:Prions / Q000378:metabolism*; D043484:Proprotein Convertases / Q000378:metabolism; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D021381:Protein Transport; D050766:Qb-SNARE Proteins / Q000235:genetics / Q000378:metabolism; D050683:R-SNARE Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D033921:Vesicular Transport Proteins / Q000235:genetics / Q000378:metabolism*; D050888:Heat-Shock Proteins, Small
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; D026905:Amino Acid Transport Systems; D056827:Endosomal Sorting Complexes Required for Transport; D006360:Heat-Shock Proteins; D011328:Prions; D043484:Proprotein Convertases; D050766:Qb-SNARE Proteins; D050683:R-SNARE Proteins; D029701:Saccharomyces cerevisiae Proteins; D033921:Vesicular Transport Proteins; C473804:BTN2 protein, S cerevisiae; D050888:Heat-Shock Proteins, Small; C083241:SNC1 protein, S cerevisiae; C560786:TDA3 protein, S cerevisiae; C106736:VTI1 protein, S cerevisiae; C473803:YIF1 protein, S cerevisiae; C070972:HsP104 protein, S cerevisiae; C058751:KEX2 protein, S cerevisiae