title: Dynamic analysis of pathogen-infected host cells using quantitative phase microscopy.
abstract: We present the real-time quantitative analysis of Vibrio vulnificus-infected host cells using quantitative phase microscopy (QPM) based on interferometric techniques. This provides the ability to retrieve the phase or optical path-length distribution over the cell with nanometer path-length sensitivity from a single interferogram image. We have used QPM to study dynamic cell morphologic changes and to noninvasively quantify the cell volumes of rat basophilic leukemia RBL-2H3 cells infected with V. vulnificus strains: wild type (MO6-24∕O) and RtxA1 toxin mutant (CMM770). During the process of V. vulnificus infection in RBL-2H3 cells, the dynamic changes of quantitative phase images, cell volumes, and areas were observed in real time using QPM. In contrast, dramatic changes were not detected in RBL-2H3 cells infected with the noncytotoxic RtxA1 toxin mutant. The results showed good correlation between QPM analysis and biochemical assays, such as lactate dehydrogenase assay or β-hexosaminidase release assay. We suggest that QPM is a powerful quantitative method to study the dynamic process of host cells infected with pathogens in a noninvasive manner.
authors: Gwangju Institute of Science and Technology, Department of Information and Communications, Buk-gu, Gwangju, Republic of Korea.
authors: Seungrag
authors: S
authors: Lee
authors: Young Ran
authors: YR
authors: Kim
authors: Ji Yong
authors: JY
authors: Lee
authors: Joon Haeng
authors: JH
authors: Rhee
authors: Chang-Soo
authors: CS
authors: Park
authors: Dug Young
authors: DY
authors: Kim
mesh_terms: D000818:Animals; D002460:Cell Line; D048429:Cell Size; D005798:Genes, Bacterial; D054884:Host-Pathogen Interactions / Q000502:physiology*; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*; D008857:Microscopy, Interference; D008858:Microscopy, Phase-Contrast; D009154:Mutation; D055096:Optical Devices; D055620:Optical Phenomena; D051381:Rats; D041261:Vibrio vulnificus / Q000235:genetics / Q000472:pathogenicity*; D014774:Virulence / Q000235:genetics