title: Ligand carrier protein genes expressed in larval chemosensory organs of Bombyx mori.
abstract: Expressed sequence tags (ESTs) of the maxillary galea of the silkworm were analyzed to identify proteins involved in food selection systems. From the 1251 redundant genes of the ESTs, we identified 7 odorant-binding protein-like genes (bmObpL), 6 takeout-like genes (bmToL), and 6 chemosensory protein genes (bmCsp). Quantitative RT-PCR analysis indicated that bmObpL1, bmObpL2, bmObpL3, bmObpL5, bmToL1, bmToL3, and bmorCsp15 were predominantly expressed in the larval oral appendages, such as the maxilla, labrum, labium and antenna. Immunocytochemical analysis indicated that the proteins of bmObpL1, bmObpL3, and bmToL1 were localized in the gustatory chemosensilla on the maxillary galea and olfactory sensilla in the antenna. The proteins encoded by bmObpL1 and bmObpL3 were detected in the gustatory chemosensilla of the epipharynx. However, bmObpL1 and bmToL1 were also detected in tactile hairs and in the epidermis of several chemosensory organs. The bmObpL2 protein was localized inside and in the epidermis around the chemosensilla, tactile hairs, and wide surface of the epipharynx. From these results, bmObpL3 is the most likely to have a dedicated role in chemoreception in the silkworm, Bombyx mori.
authors: Graduate School of Bio-Application and Systems Engineering, Tokyo University of Agriculture and Technology, Koganei 2-24-16, Tokyo 184-8588, Japan.
authors: Yasutaka
authors: Y
authors: Yoshizawa
authors: Ryoichi
authors: R
authors: Sato
authors: Kazuko
authors: K
authors: Tsuchihara
authors: Katsuhisa
authors: K
authors: Ozaki
authors: Kazuei
authors: K
authors: Mita
authors: Kiyoshi
authors: K
authors: Asaoka
authors: Kiyoko
authors: K
authors: Taniai
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D057506:Arthropod Antennae / Q000166:cytology / Q000378:metabolism; D015153:Blotting, Western; D012831:Bombyx / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D002628:Chemoreceptor Cells / Q000166:cytology / Q000378:metabolism*; D003001:Cloning, Molecular; D004926:Escherichia coli; D020224:Expressed Sequence Tags; D005518:Food Preferences / Q000502:physiology; D020869:Gene Expression Profiling; D015723:Gene Library; D049750:Genome, Insect; D007150:Immunohistochemistry; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D007814:Larva / Q000166:cytology / Q000235:genetics* / Q000378:metabolism; D008024:Ligands; D008969:Molecular Sequence Data; D005810:Multigene Family; D010802:Phylogeny; D010957:Plasmids; D018035:Receptors, Odorant / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction; D058618:Sensilla / Q000166:cytology / Q000378:metabolism*; D016415:Sequence Alignment
chemical_list: D019476:Insect Proteins; D008024:Ligands; D018035:Receptors, Odorant; D011994:Recombinant Proteins; C045648:odorant-binding protein