title: Topical liposomal DNA-repair enzymes in polymorphic light eruption.
abstract: Polymorphic light eruption (PLE) is a very frequent photodermatosis in Europe whose pathogenesis may involve resistance to UV-induced immune suppression and simultaneous immune reactions against skin photoneoantigens. We performed a randomized, double-blind, placebo-controlled intra-individual half-body trial to investigate the protective effect of an after-sun (AS) lotion containing DNA-repair enzymes (photolyase from Anacystis nidulans and Micrococcus luteus extract with endonuclease activity). Fourteen PLE patients were exposed to suberythemal doses of solar-simulated UV radiation on 4 consecutive days at 4 symmetrically located PLE-prone test fields per patient. The test fields were treated with (i) active AS lotion or (ii) a placebo lotion immediately after each UV exposure, or (iii) an SPF30 sunscreen before UV exposure or left untreated. All test fields were exposed to photoactivating blue light 1 h after each UV exposure. As shown by a newly established specific PLE test score (AA + SI + 0.4P [range, 0-12], where AA is affected area score [range, 0-4], SI is skin infiltration score [range, 0-4], and P is pruritus score on a visual analogue scale [range, 0-10]), PLE symptoms were significantly fewer on test sites treated with active AS lotion than on untreated (P = 0.00049) or placebo-treated test sites (P = 0.024). At 144 h after first UV exposure (the time point of maximal PLE symptoms), the mean test scores for untreated, active AS lotion-treated, and placebo-treated test fields were 4.39, 1.73 (61% reduction; 95% confidence interval (CI), 36% to 85%), and 3.20 (27% reduction; 95% CI, 3% to 51%), respectively. Pretreatment with SPF30 sunscreen completely prevented PLE symptoms in all patients. The present results indicate that DNA damage may trigger PLE and that the application of topical liposomes containing DNA repair enzymes to increase DNA repair may effectively prevent PLE.
authors: Research Unit for Photodermatology, Department of Dermatology, Medical University of Graz, Auenbrugger Platz 8, A-8036, Graz, Austria.
authors: Angelika
authors: A
authors: Hofer
authors: Franz J
authors: FJ
authors: Legat
authors: Alexandra
authors: A
authors: Gruber-Wackernagel
authors: Franz
authors: F
authors: Quehenberger
authors: Peter
authors: P
authors: Wolf
mesh_terms: D000287:Administration, Topical; D000328:Adult; D000368:Aged; D004249:DNA Damage; D004255:Deoxyribodipyrimidine Photo-Lyase / Q000008:administration & dosage* / Q000737:chemistry; D004311:Double-Blind Method; D005260:Female; D006801:Humans; D008081:Liposomes; D008297:Male; D016982:Micrococcus luteus / Q000201:enzymology; D008875:Middle Aged; D010787:Photosensitivity Disorders / Q000473:pathology / Q000517:prevention & control*; D015990:Placebo Effect; D011446:Prospective Studies; D013473:Sunscreening Agents / Q000008:administration & dosage*; D046940:Synechococcus / Q000201:enzymology; D014466:Ultraviolet Rays
chemical_list: D008081:Liposomes; D013473:Sunscreening Agents; D004255:Deoxyribodipyrimidine Photo-Lyase