title: Study of DNA interaction with cobalt ferrite nanoparticles.
abstract: Interaction of cobalt ferrite nanopowder and nucleic acid was investigated. Superparamagnetic cobalt ferrite nanoparticles (6-12 nm) were prepared by mechanochemical synthesis. Structure of the nanopowder was characterized using X-ray diffraction. It was shown that cobalt ferrite nanoparticles were associated with ssDNA and dsDNA in Tris-buffer resulting in bionanocomposite formation with mass weight relation nanoparticles: DNA 1:(0.083 +/- 0.003) and 1:(0.075 +/- 0.003) respectively. The mechanism of interaction between a DNA and cobalt ferrite nanoparticles was considered basing on the whole set of obtained data: FTIR-spectroscopy, analyzing desorption of DNA from the surface of the particles while changing the chemical content of the medium, and on the modeling interaction of specific biomolecule fragments with surface of a inorganic material. It was supposed that the linkage was based on coordination interaction of the phosphate groups and oxygen atoms heterocyclic bases of DNA with metal ions on the particle surface. These data can be used to design specific magnetic DNA-nanoparticles hybrid structures.
authors: Siberian State Medical University, Moscowski Trakt, 2, 634050 Tomsk, Russia.
authors: A G
authors: AG
authors: Pershina
authors: A E
authors: AE
authors: Sazonov
authors: D V
authors: DV
authors: Novikov
authors: A S
authors: AS
authors: Knyazev
authors: T I
authors: TI
authors: Izaak
authors: V I
authors: VI
authors: Itin
authors: E P
authors: EP
authors: Naiden
authors: A A
authors: AA
authors: Magaeva
authors: O G
authors: OG
authors: Terechova
mesh_terms: D003035:Cobalt / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D005290:Ferric Compounds / Q000737:chemistry*; D046911:Macromolecular Substances / Q000737:chemistry; D008280:Magnetics; D008422:Materials Testing; D008968:Molecular Conformation; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D010316:Particle Size; D013499:Surface Properties
chemical_list: D005290:Ferric Compounds; D046911:Macromolecular Substances; C001215:ferrite; D003035:Cobalt; D004247:DNA