title: [Effect of bushen tiaojing recipe and xiaoyao pill on expression of cathepsin-L mRNA in gonadotropin-primed mice].
abstract: OBJECTIVE
  To study the effect of Bushen Tiaojing Recipe (BTR) and Xiaoyao Pill (XP) on cathepsin-L (Cat-L) mRNA in mice.
  
  
  METHODS
  Immature mice were randomly divided into the normal group, the control group, the BTR group and the XP group, three in each group. Cat-L mRNA expression in mice was detected using reverse transcription polymerase chain reaction (RT-PCR) at 0, 4, 8 and 12 h after injecting 5 IU (human chorionic gonadotropin, HCG).
  
  
  RESULTS
  Cat-L mRNA expression increased gradually after HCG injection, the relative levels in the control group at 0, 4, 8 and 12 h were 0.066 +/- 0.005, 0.383 +/- 0.045, 0.737 +/- 0.024 and 1.036 +/- 0.073 respectively, comparisons between different time-points showed significant difference (P < 0.01). Compared with the control group, the Cat L mRNA expression was higher at 4 h in both BTR and XP groups (P < 0.01), at 8 h in the XP group (P < 0.05), and at 12 h in BTR group after injecting HCG (P < 0.05). Compared with the control group, cat L mRNA expression showed no statistic difference at 8 h in BTR group and at 12 h in XC group.
  
  
  CONCLUSIONS
  BTR promoted the ovulation by enhancing the expression of CatL gene, and that of XP by advancing the peak of CatL gene expression.
authors: Institute of Integrated Chinese and Western Medicine, Hebei Medical University, Shijiazhuang.
authors: Yan-cang
authors: YC
authors: Duan
authors: Hui-lan
authors: HL
authors: Du
authors: Ming
authors: M
authors: He
mesh_terms: D000818:Animals; D056668:Cathepsin L / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D006062:Gonadotropins / Q000008:administration & dosage; D006801:Humans; D051379:Mice; D008815:Mice, Inbred Strains; D010060:Ovulation / Q000187:drug effects*; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D004365:Drugs, Chinese Herbal; D006062:Gonadotropins; D012333:RNA, Messenger; C516449:xiaoyao; D056668:Cathepsin L; C534278:Ctsl protein, mouse