title: Ex vivo expansion of human mesenchymal stem cells on microcarriers.
abstract: Due to the very low titers of human mesenchymal stem cells (MSC) in their niches, namely the bone marrow, an effective approach to isolate and expand those cells ex vivo is required to meet the needs of the increasing MSC clinical applications (e.g., therapy-resistant severe acute graft-versus-host disease). Herein we describe a microcarrier-based stirred culture system protocol for the efficient ex vivo expansion of human bone marrow-derived MSC. This protocol is potentially adaptable to different culture conditions, namely focusing the use of serum-free medium formulations, other sources of MSC, or different types of microcarriers.
authors: IBB-Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Técnico, Lisboa, Portugal.
authors: Francisco
authors: F
authors: dos Santos
authors: Pedro Z
authors: PZ
authors: Andrade
authors: Gemma
authors: G
authors: Eibes
authors: Cláudia Lobato
authors: CL
authors: da Silva
authors: Joaquim M S
authors: JM
authors: Cabral
mesh_terms: D050156:Adipogenesis; D000423:Alcian Blue / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D001391:Azo Compounds / Q000378:metabolism; D002452:Cell Count; D018929:Cell Culture Techniques / Q000295:instrumentation*; D002470:Cell Survival; D020219:Chondrogenesis; D016895:Culture Media, Serum-Free; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D010012:Osteogenesis; D046888:Tissue Array Analysis / Q000379:methods*
chemical_list: D001391:Azo Compounds; D016895:Culture Media, Serum-Free; D000469:Alkaline Phosphatase; C011049:oil red O; D000423:Alcian Blue