title: Pseudoxanthomonas jiangsuensis sp. nov., a DDT-degrading bacterium isolated from a long-term DDT-polluted soil.
abstract: A Gram-negative, strictly aerobic, rod-shaped bacterium, designated strain wax(T), was isolated from DDT-contaminated soil in Yangzhou, China. Growth of strain wax(T) was observed at 10-45°C (optimum 30°C) and pH 5.0-10.0 (optimum pH 7.0-8.0). The predominant fatty acids were iso C(15:0) (32.21%) and anteiso C(15:0) (22.2%). The strain contained large amounts of the polar lipids diphosphatidylglycerol, phosphatidylethanolamine, and phosphatidylglycerol, but small amounts of an unknown amino-group-containing polar lipid and phospholipids were also present. The major quinone was ubiquinone-8 (Q-8) and the G + C content of the genomic DNA was 67.12 ± 0.8 mol.%. The phylogenetic tree shows that strain wax(T) clustered within the genus Pseudoxanthomonas. Isolate wax(T) showed moderate 16S rRNA gene sequence similarities to Pseudoxanthomonas broegbernensis B1616/1(T) (98.3%), P. suwonensis 4M1(T) (98.2%), P. daejeonensis TR6-08(T) (98%), P. koreensis TR7-09(T) (97.7%), P. kaohsiungensis J36(T) (97.5%), P. mexicana AMX 26B(T) (97.2%), and P. japonensis 12-3(T) (97%). The level of DNA-DNA relatedness between strain wax(T) and Pseudoxanthomonas type strains were low, e.g., P. koreensis TR7-09(T) (25.9%), P. broegbernensis B1616/1(T) (36.4%), P. suwonensis 4M1(T) (27.7%), P. daejeonensis TR6-08(T) (40%), P. kaohsiungensis J36(T) (20.4%), P. mexicana AMX 26B(T) (29.0%), P. japonensis 12-3(T) (33.3%). On the basis of the phenotypic, chemotaxonomic data and molecular properties, strain wax(T) represents a novel species within the genus Pseudoxanthomonas, for which the name Pseudoxanthomonas jiangsuensis sp. nov. is proposed. The type strain is wax(T) (=DSM 22398(T) = CGMCC 1.10137(T)).
authors: Key Laboratory of Microbiological Engineering of Agricultural Environment, Department of Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, Jiangsu, People's Republic of China.
authors: Guang-li
authors: GL
authors: Wang
authors: Meng
authors: M
authors: Bi
authors: Bin
authors: B
authors: Liang
authors: Jian-dong
authors: JD
authors: Jiang
authors: Shun-peng
authors: SP
authors: Li
mesh_terms: D015373:Bacterial Typing Techniques; D001673:Biodegradation, Environmental; D003634:DDT / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D007306:Insecticides / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*; D044166:Xanthomonadaceae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D007306:Insecticides; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; D003634:DDT