title: One-pot synthesis of cycloamyloses from sucrose by dual enzyme treatment: combined reaction of amylosucrase and 4-α-glucanotransferase.
abstract: Amylose-like α-(1,4)-glucan known as the most favorable substrate for the enzymatic production of cycloamyloses (CAs) using 4-α-glucanotransferase has a solubility issue, which requires an additional solubilization process. In our study, two glucosyltransferases, Synechocystis 4-α-glucanotransferase and Neisseria amylosucrase, were adopted to develop an efficient biocatalytic production process of CAs directly from sucrose. From one-pot synthesis, the maximum CA yield (9.6%, w/w) with 0.3 M sucrose was achieved with 10 units/mL of amylosucrase and 0.1 unit/mL of 4-α-glucanotransferase at 40 °C for a 3 h reaction in a simultaneous dual enzyme reaction mode. The size of linear α-(1,4)-glucan was positively related to the CA productivity by 4-α-glucanotransferase in a hyperbolic manner. Using our innovative bioprocess, there was no practical limitation on the initial sucrose concentration and no use of insoluble linear α-(1,4)-glucan substrate. Consequently, the concomitant dual enzyme reaction converted sucrose directly to CAs via in situ transient linear α-(1,4)-glucan as an soluble intermediate.
authors: Department of Food Science and Technology, and Carbohydrate Bioproduct Research Center, Sejong University, Seoul, Korea.
authors: Jung-Hwan
authors: JH
authors: Kim
authors: Ren
authors: R
authors: Wang
authors: Wang-Hee
authors: WH
authors: Lee
authors: Cheon-Seok
authors: CS
authors: Park
authors: Suyong
authors: S
authors: Lee
authors: Sang-Ho
authors: SH
authors: Yoo
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D055162:Biocatalysis; D003505:Cyclodextrins / Q000138:chemical synthesis*; D005964:Glucosyltransferases / Q000737:chemistry*; D006004:Glycogen Debranching Enzyme System / Q000737:chemistry*; D009343:Neisseria / Q000201:enzymology*; D013395:Sucrose / Q000737:chemistry*; D046939:Synechocystis / Q000201:enzymology*
chemical_list: D001426:Bacterial Proteins; D003505:Cyclodextrins; D006004:Glycogen Debranching Enzyme System; D013395:Sucrose; D005964:Glucosyltransferases; C020569:4 alpha-glucanotransferase; C019700:amylosucrase