title: Antiviral activity of bovine type III interferon against foot-and-mouth disease virus.
abstract: Foot-and-mouth disease (FMD) is one of the most serious threats to the livestock industry. Despite the availability of a vaccine, recent outbreaks in disease-free countries have demonstrated that development of novel FMD control strategies is imperative. Here we report the identification and characterization of bovine (bo) interferon lambda 3 (IFN-λ3), a member of the type III IFN family. Expression of boIFN-λ3 using a replication-defective human adenovirus type 5 vector (Ad5-boIFN-λ3) yielded a glycosylated secreted protein with antiviral activity against FMD virus (FMDV) and vesicular stomatitis virus in bovine cell culture. Inoculation of cattle with Ad5-boIFN-λ3 induced systemic antiviral activity and up-regulation of IFN stimulated gene expression in multiple tissues susceptible to FMDV infection. Our results demonstrate that the type III IFN family is conserved in bovines and boIFN-λ3 has potential for further development as a biotherapeutic candidate to inhibit FMDV or other viruses in cattle.
authors: Plum Island Animal Disease Center, North Atlantic Area, Agricultural Research Service, U.S. Department of Agriculture, Greenport, NY 11944, USA.
authors: Fayna
authors: F
authors: Díaz-San Segundo
authors: Marcelo
authors: M
authors: Weiss
authors: Eva
authors: E
authors: Perez-Martín
authors: Marla J
authors: MJ
authors: Koster
authors: James
authors: J
authors: Zhu
authors: Marvin J
authors: MJ
authors: Grubman
authors: Teresa
authors: T
authors: de los Santos
mesh_terms: D000260:Adenoviruses, Human / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D000998:Antiviral Agents / Q000378:metabolism / Q000494:pharmacology*; D001483:Base Sequence; D002417:Cattle; D002460:Cell Line; D003001:Cloning, Molecular; D029544:Foot-and-Mouth Disease Virus / Q000187:drug effects*; D005786:Gene Expression Regulation; D015316:Genetic Therapy; D006801:Humans; D007372:Interferons / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D008297:Male; D008969:Molecular Sequence Data
chemical_list: D000998:Antiviral Agents; D007372:Interferons