title: Simultaneous determination of eight β-lactam antibiotics in human serum by liquid chromatography-tandem mass spectrometry.
abstract: A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the simultaneous determination of eight β-lactam antibiotics, including ampicillin, cefazolin, cefepime, cefmetazole, cefotaxime, doripenem, meropenem, and piperacillin, in human serum. Sample specimens were subjected to solid phase extraction (SPE) using Waters Oasis® HLB cartridges (30 mg). Chromatographic separation was performed with a high-resolution octadecyl silica column compatible with hydrophilic compounds, using a gradient of 10mM aqueous ammonium formate containing 0.1% formic acid-methanol. Antibiotics were detected by a triple quadrupole mass spectrometer (MS/MS) with electrospray ionization and quantified by the multiple reaction monitoring mode. A total run time of 13 min was applied. Linearity in the calibration was obtained over a range of 0.1-50 μg/mL of the β-lactam antibiotics, except for doripenem. The lower limit of quantification was 0.005-0.5 μg/mL, using 50 μL serum. The recovery rate exceeded 80.2% for these analytes, except for doripenem (49.1%) and meropenem (62.3%). The present method is applicable to routine therapeutic monitoring of β-lactam antibiotics in clinical practice.
authors: Department of Pharmacy, Gifu University Hospital, Gifu, Japan. t_ohmori@gifu-u.ac.jp
authors: Tomofumi
authors: T
authors: Ohmori
authors: Akio
authors: A
authors: Suzuki
authors: Takashi
authors: T
authors: Niwa
authors: Hiroaki
authors: H
authors: Ushikoshi
authors: Kunihiro
authors: K
authors: Shirai
authors: Shozo
authors: S
authors: Yoshida
authors: Shinji
authors: S
authors: Ogura
authors: Yoshinori
authors: Y
authors: Itoh
mesh_terms: D000900:Anti-Bacterial Agents / Q000097:blood* / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D016014:Linear Models; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry / Q000379:methods*; D047090:beta-Lactams / Q000097:blood* / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D047090:beta-Lactams