title: Characterization of apoptotic changes induced by yessotoxin in the Bel7402 human hepatoma cell line.
abstract: The apoptotic effects of yessotoxin (YTX) on Bel7402 human hepatoma cells have been evaluated by the combination of several methods, including optical microscopy, Hoechst 33342 staining and DNA gel electrophoresis. Rhodamine 123 staining has also been used to investigate changes in mitochondrial transmembrane potential. The results indicated that YTX has negative effects on human liver cells and induces apoptosis. Furthermore, changes in calcium concentrations were analyzed using the fluorescence probe Fluo-3 AM. The results showed that intracellular calcium concentrations increased after treatment with YTX.
authors.affiliation: First Institute of Oceanography, State Oceanic Administration, Qingdao, Shandong 266061, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Pang
authors.forename: Zong-Ling
authors.initials: ZL
authors.lastname: Wang
authors.forename: Chun-Lei
authors.initials: CL
authors.lastname: Gao
authors.forename: Pei
authors.initials: P
authors.lastname: Qu
authors.forename: Hai-Dong
authors.initials: HD
authors.lastname: Li
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D002118:Calcium / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000201:enzymology / Q000473:pathology*; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism; D008113:Liver Neoplasms / Q000201:enzymology / Q000473:pathology*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008978:Mollusk Venoms; D019319:Okadaic Acid / Q000494:pharmacology; D035964:Oxocins / Q000494:pharmacology*
chemical_list: D008978:Mollusk Venoms; D035964:Oxocins; D019319:Okadaic Acid; D053148:Caspase 3; C066632:yessotoxin; D002118:Calcium