title: Suppression subtractive hybridization reveals differential gene expression in sunflower grown in high P.
abstract: Sunflower (Helianthus annuus L.) is a commercially important oilseed crop. Previous studies proved that this crop is a promising plant species for phytoextraction of excess soil phosphorus (P) because of its superior P accumulating characteristics. Suppression subtractive hybridization (SSH) strategy was employed to isolate and characterize genes that are induced in response to high P in this crop. SSH library was prepared using cDNA generated from plants treated with high P as the 'tester'. Based on the results of dot blot analysis, 360 positive cDNA clones were selected from the SSH library for sequencing. A total of 89 non-redundant expressed sequence tags (ESTs) were identified as high P-responsive genes and they were classified into 6 functional groups. Several genes involved in metabolism showed markedly preferential expression in the library. For further confirmation, thirteen of the representative ESTs were selected from all categories for RT-PCR analysis and the results showed up-regulation of these genes in response to high P-treatment. The gene expression data derived from this study suggested that several of the up-regulated genes identified under high P-treatment might be involved in P-accumulation and tolerance in this plant.
authors.affiliation: Department of Biology, Western Kentucky University, 1906 College Heights Blvd #110180, Bowling Green, KY 42101-1080, USA.
authors.forename: Priya
authors.initials: P
authors.lastname: Padmanabhan
authors.forename: Shivendra V
authors.initials: SV
authors.lastname: Sahi
mesh_terms: D018076:DNA, Complementary; D020224:Expressed Sequence Tags; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D017343:Genes, Plant / Q000502:physiology*; D006368:Helianthus / Q000235:genetics* / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D010758:Phosphorus / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation
chemical_list: D018076:DNA, Complementary; D010758:Phosphorus