title: Inhibiting ERp29 expression enhances radiosensitivity in human nasopharyngeal carcinoma cell lines.
abstract: ERp29 is an endoplasmic reticulum (ER) stress-inducible protein. It was found that ERp29 was highly expressed in several cancers and associated with resistance to oxidative and radiation stress, which may serve as a novel target for nasopharyngeal carcinoma (NPC) anticancer approach. In this study, we used immunohistochemistry to detect ERp29 expression in radioresistant and radiosensitive NPC tissues. As a result, ERp29 was up-regulated in radioresistant NPC tissues compared to radiosensitive NPC tissues. We also found that ERp29 knockdown attenuated radioresistance of NPC CNE-1 cells and ERp29 overexpression enhanced radioresistance of NPC CNE-2 cells. When exposed to radiation, ERp29 knockdown CNE-1 cells increased radiation-induced cell apoptosis and ERp29 overexpression CNE-2 cells reduced radiation-induced cell apoptosis. Further, we demonstrated that ERp29 up-regulated the expression of Hsp27. In conclusion, our study supports ERp29 could potentiate resistance to radiation in NPC cells, targeting of ERp29 is a rational strategy in treating radioresistant NPC.
authors.affiliation: Department of Otolaryngology, Xiangya Hospital, Central South University, Xiangya Road 87, Changsha 410008, Hunan Province, People's Republic of China.
authors.forename: Lin
authors.initials: L
authors.lastname: Qi
authors.forename: Ping
authors.initials: P
authors.lastname: Wu
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Yuanzheng
authors.initials: Y
authors.lastname: Qiu
authors.forename: Weihong
authors.initials: W
authors.lastname: Jiang
authors.forename: Donghai
authors.initials: D
authors.lastname: Huang
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.forename: Pingqing
authors.initials: P
authors.lastname: Tan
authors.forename: Yongquan
authors.initials: Y
authors.lastname: Tian
mesh_terms: D017209:Apoptosis / Q000528:radiation effects*; D015153:Blotting, Western; D002277:Carcinoma; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D055551:HSP27 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007124:Immunoenzyme Techniques; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000532:radiotherapy*; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D011836:Radiation Tolerance / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
chemical_list: C491738:ERP29 protein, human; D055551:HSP27 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D012333:RNA, Messenger; D034741:RNA, Small Interfering