title: Simian varicella virus open reading frame 63/70 expression is required for efficient virus replication in culture.
abstract: Simian varicella virus (SVV) open reading frame (ORF) 63, duplicated in the virus genome as ORF 70, is homologous to varicella zoster virus ORF 63/70. Transfection of bacterial artificial chromosome clones containing the wild-type SVV genome and mutants with stop codons in ORF 70, in both ORFs 63 and 70 and the repaired virus DNA sequences into Vero cells produced a cytopathic effect (CPE). The onset of CPE was much slower with the double-mutant transfectants (10 days vs. 3 days) and plaques were smaller. While SVV ORF 63 is not required for replication in culture, its expression leads to robust virus replication.
authors.affiliation: Department of Neurology, University of Colorado School of Medicine, 12700 E. 19th Avenue, Box B182, Aurora, CO 80045, USA.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Brazeau
authors.forename: Mary
authors.initials: M
authors.lastname: Wellish
authors.forename: Benedict B
authors.initials: BB
authors.lastname: Kaufer
authors.forename: B Karsten
authors.initials: BK
authors.lastname: Tischer
authors.forename: Wayne
authors.initials: W
authors.lastname: Gray
authors.forename: Fuchun
authors.initials: F
authors.lastname: Zhou
authors.forename: Nikolaus
authors.initials: N
authors.lastname: Osterrieder
authors.forename: Teri
authors.initials: T
authors.lastname: Hanlon
authors.forename: Anjani
authors.initials: A
authors.lastname: Golive
authors.forename: Travis
authors.initials: T
authors.lastname: Hall
authors.forename: Sreekala
authors.initials: S
authors.lastname: Nair
authors.forename: Gregory P
authors.initials: GP
authors.lastname: Owens
authors.forename: Niklaus H
authors.initials: NH
authors.lastname: Mueller
authors.forename: Randall J
authors.initials: RJ
authors.lastname: Cohrs
authors.forename: Subbiah
authors.initials: S
authors.lastname: Pugazhenthi
authors.forename: Don
authors.initials: D
authors.lastname: Gilden
authors.forename: Ravi
authors.initials: R
authors.lastname: Mahalingam
mesh_terms: D000818:Animals; D001483:Base Sequence; D002644:Chickenpox / Q000235:genetics* / Q000821:virology*; D002522:Chlorocebus aethiops; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics* / Q000378:metabolism; D003588:Cytopathogenic Effect, Viral / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D016679:Genome, Viral; D014645:Herpesvirus 3, Human / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D016366:Open Reading Frames; D014162:Transfection; D014709:Vero Cells; D014779:Virus Replication / Q000235:genetics
chemical_list: D004279:DNA, Viral