title: [Effect of endophytic fungal elicitors on growth and atractylodin accumulation of cell suspension cultures of Atractylodes lancea].
abstract: OBJECTIVE
  To investigate the effects of endophytic fungal elicitors on the growth and atractylodin accumulation of cell suspension cultures of Atractylodes lancea.
  
  
  METHODS
  The endophytic fungal elicitors were added to the medium with different concentrations and culture period. Their effects on biomass, atractylodin content and relevant enzyme activities in suspension cultured cells were studied.
  
  
  RESULTS
  The cell growth was not affected by elicitors at low concentration and obviously inhibited at high concentration. Inhibition rate reached 46.7% by 100 mg L(-1) elicitor. In addition, six strains from A. lancea, among which Rhizoctonia SP1 activity was higher, had distinctly promoted the accumulation of atractylodin. Atractylodin biosynthesis was notably promoted by 20-60 mg L(-1) Rhizoctonia SP1 elicitor. When 40 mg L(-1) Rhizoctonia SP1 elicitor was added in the medium at the 12 day, the maximum content of atractylodin was 28.06 microg L(-1) at the 21 day with 48.3% higher than that of the control and PPO, POD and CAT activities remarkably increased.
  
  
  CONCLUSIONS
  Adding the endophytic elicitors to the medium is able to be effective approaches to enhance atractylodin yield in the suspension culture cell of A. lancea.
authors.affiliation: Medical School, Nantong University, Nantong 226001, China. taojinhua2000@163.com
authors.forename: Jinhua
authors.initials: J
authors.lastname: Tao
authors.forename: Xuelian
authors.initials: X
authors.lastname: Pu
authors.forename: Shu
authors.initials: S
authors.lastname: Jiang
mesh_terms: D031174:Atractylodes / Q000254:growth & development* / Q000382:microbiology* / Q000502:physiology; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D005658:Fungi / Q000502:physiology*; D005663:Furans / Q000378:metabolism*; D013559:Symbiosis
chemical_list: D005663:Furans; C106914:atractylodin