title: Diagnosis of botulism types C and D in cattle by a monoclonal antibody-based sandwich ELISA.
abstract: This study was undertaken to evaluate two monoclonal antibody-based sandwich ELISAs (sELISAs) for the detection of Clostridium botulinum neurotoxins (BoNTs) types C and D from culture-enriched intestinal content samples from cattle. To validate the diagnostic significance of the presence of cultivable, toxin-producing C botulinum in the intestines of cattle, samples from both suspect and non-suspect botulism cases were examined. BoNT was detected by both sELISAs in a greater number of suspect animals than by direct testing of uncultured samples by mouse bioassay. One sELISA detected two BoNT C and one BoNT Group III mosaic isoform in three animals that were missed by the other, and both sELISAs failed to identify samples from two mouse bioassay-positive BoNT C animals. BoNT D was also detected in one non-suspect sample by one of the sELISAs.
authors.affiliation: Veterinary Sciences Division, Agri-Food and Biosciences Institute Northern Ireland, Stormont, Belfast. cathy.brooks@afbini.gov.uk
authors.forename: C E
authors.initials: CE
authors.lastname: Brooks
authors.forename: H J
authors.initials: HJ
authors.lastname: Clarke
authors.forename: D A
authors.initials: DA
authors.lastname: Graham
authors.forename: H J
authors.initials: HJ
authors.lastname: Ball
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001681:Biological Assay; D001905:Botulinum Toxins / Q000276:immunology; D019274:Botulinum Toxins, Type A; D001906:Botulism / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000276:immunology; D003014:Clostridium botulinum / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D007422:Intestines / Q000382:microbiology; D051379:Mice; D013171:Spores, Bacterial / Q000276:immunology / Q000302:isolation & purification
chemical_list: D000911:Antibodies, Monoclonal; C096323:rimabotulinumtoxinB; D001905:Botulinum Toxins; D019274:Botulinum Toxins, Type A; C096324:botulinum toxin type C