title: A quantitative literature-curated gold standard for kinase-substrate pairs.
abstract: We describe the Yeast Kinase Interaction Database (KID, http://www.moseslab.csb.utoronto.ca/KID/), which contains high- and low-throughput data relevant to phosphorylation events. KID includes 6,225 low-throughput and 21,990 high-throughput interactions, from greater than 35,000 experiments. By quantitatively integrating these data, we identified 517 high-confidence kinase-substrate pairs that we consider a gold standard. We show that this gold standard can be used to assess published high-throughput datasets, suggesting that it will enable similar rigorous assessments in the future.
authors.affiliation: Department of Molecular Genetics, The Donnelly Centre for Cellular and Biomolecular Research, University of Toronto,160 College Street, Toronto, M3S 3E1, Canada.
authors.forename: Sara
authors.initials: S
authors.lastname: Sharifpoor
authors.forename: Alex N
authors.initials: AN
authors.lastname: Nguyen Ba
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Youn
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Young
authors.forename: Dewald
authors.initials: D
authors.lastname: van Dyk
authors.forename: Helena
authors.initials: H
authors.lastname: Friesen
authors.forename: Alison C
authors.initials: AC
authors.lastname: Douglas
authors.forename: Christoph F
authors.initials: CF
authors.lastname: Kurat
authors.forename: Yolanda T
authors.initials: YT
authors.lastname: Chong
authors.forename: Karen
authors.initials: K
authors.lastname: Founk
authors.forename: Alan M
authors.initials: AM
authors.lastname: Moses
authors.forename: Brenda J
authors.initials: BJ
authors.lastname: Andrews
mesh_terms: D030562:Databases, Protein; D011494:Protein Kinases / Q000145:classification / Q000378:metabolism*; D012015:Reference Standards; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D013379:Substrate Specificity
chemical_list: D011494:Protein Kinases