title: The expression of B7-H1 and B7-H4 molecules on immature myeloid and lymphoid dendritic cells in cord blood of healthy neonates.
abstract: The aim of our study was to estimate both B7-H1 and B7-H4 molecules on immature myeloid and lymphoid dendritic cells in umbilical cord blood of healthy neonates in comparison with peripheral blood of healthy adults. Thirty nine healthy full-term neonates from physiological single pregnancies and 27 healthy adults were included in the study. The expression of B7-H1 and B7-H4 was revealed using the immunofluorescence method. Statistical analysis was performed using a non-parametric test (Mann-Whitney U-Test). The percentages of BDCA-1+ dendritic cells with B7-H1 and B7-H4 expressions were significantly higher in peripheral blood of healthy adults (p<0.00003). It was either observed that the percentage of BDCA-2+ dendritic cells with the expression of B7-H4 molecules was significantly higher in peripheral blood of healthy adults in comparison with umbilical cord blood (p<0.02). Decreased percentages of dendritic cells and co-stimulatory molecules indicate that neonates have immature immune system. Depletion of co-stimulatory B7-H1 and B7-H4 molecules enable appropriate development of immune response.
authors.affiliation: Department of Clinical Immunology, Medical University of Lublin, Poland. magdalenakludka@go2.pl
authors.forename: M
authors.initials: M
authors.lastname: Kludka-Sternik
authors.forename: A
authors.initials: A
authors.lastname: Serafin
authors.forename: D
authors.initials: D
authors.lastname: Darmochwal-Kolarz
authors.forename: S
authors.initials: S
authors.lastname: Radej
authors.forename: J
authors.initials: J
authors.lastname: Rolinski
authors.forename: B
authors.initials: B
authors.lastname: Leszczynska-Gorzelak
authors.forename: J
authors.initials: J
authors.lastname: Oleszczuk
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000378:metabolism*; D018122:B7-1 Antigen / Q000378:metabolism*; D060890:B7-H1 Antigen; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism*; D005312:Fetal Blood / Q000378:metabolism*; D005434:Flow Cytometry; D006801:Humans; D007231:Infant, Newborn; D037181:Lectins, C-Type / Q000378:metabolism; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000378:metabolism; D022423:Myeloid Cells / Q000276:immunology / Q000378:metabolism*; D011971:Receptors, Immunologic / Q000378:metabolism; D060907:V-Set Domain-Containing T-Cell Activation Inhibitor 1
chemical_list: D015703:Antigens, CD; D018122:B7-1 Antigen; D060890:B7-H1 Antigen; C423236:CD274 protein, human; C443299:CLEC4C protein, human; D037181:Lectins, C-Type; D008562:Membrane Glycoproteins; D011971:Receptors, Immunologic; D060907:V-Set Domain-Containing T-Cell Activation Inhibitor 1; C475826:VTCN1 protein, human