title: Peptide affinity reagents for AAV capsid recognition and purification.
abstract: We report the discovery of AAV capsid-binding peptides identified through phage panning. The heptapeptide motif GYVSRHP selectively recognized AAV serotype 8 capsids and blocked transduction in vitro. Recombinant AAV8 vectors were purified directly from crude cell lysate and supernatant through sequential application of peptide affinity and anion exchange chromatography. Peptide affinity reagents may serve as useful alternatives to monoclonal antibodies in AAV capsid recognition, and offer readily scalable solutions for purification of clinical grade AAV vectors.
authors.affiliation: Gene Therapy Center, The University of North Carolina, Chapel Hill, NC 27599, USA.
authors.forename: N
authors.initials: N
authors.lastname: Pulicherla
authors.forename: A
authors.initials: A
authors.lastname: Asokan
mesh_terms: D020816:Amino Acid Motifs / Q000235:genetics; D002213:Capsid / Q000378:metabolism*; D002460:Cell Line; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D017931:DNA Primers / Q000235:genetics; D000229:Dependovirus / Q000378:metabolism*; D005822:Genetic Vectors / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D010455:Peptides / Q000235:genetics / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D010455:Peptides