title: Detection of DNA hybridisation in a diluted serum matrix by surface plasmon resonance and film bulk acoustic resonators.
abstract: Nanomolar quantities of single-stranded DNA products ~100 nucleotides long can be detected in diluted 1% serum by surface plasmon resonance (SPR) and film bulk acoustic resonators (FBARs). We have used a novel FBAR sensor in parallel with SPR and obtained promising results with both the acoustic and the optical device. Oligonucleotides and a repellent lipoamide, Lipa-DEA, were allowed to assemble on the sensor chip surfaces for only 15 min by dispensing. Lipa-DEA surrounds the analyte-binding probes on the surface and effectively reduces the non-specific binding of bovine serum albumin and non-complementary strands. In a highly diluted serum matrix, the non-specific binding is, however, a hindrance, and the background response must be reduced. Nanomolar concentrations of short complementary oligos could be detected in buffer, whereas the response was too low to be measured in serum. DNA strands that are approximately 100 base pairs long at concentrations as low as 1-nM could be detected both in buffer and in 1% serum by both SPR and the FBAR resonator.
authors.affiliation: VTT Technical Research Centre of Finland, P.O. Box 1300, 33101 Tampere, Finland. Sanna.Auer@vtt.fi
authors.forename: Sanna
authors.initials: S
authors.lastname: Auer
authors.forename: Martin
authors.initials: M
authors.lastname: Nirschl
authors.forename: Matthias
authors.initials: M
authors.lastname: Schreiter
authors.forename: Inger
authors.initials: I
authors.lastname: Vikholm-Lundin
mesh_terms: D000162:Acoustics; D015374:Biosensing Techniques / Q000379:methods*; D015342:DNA Probes / Q000737:chemistry; D004277:DNA, Single-Stranded / Q000032:analysis*; D006801:Humans; D008297:Male; D009693:Nucleic Acid Hybridization / Q000379:methods*; D012680:Sensitivity and Specificity; D044967:Serum / Q000737:chemistry*; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D015342:DNA Probes; D004277:DNA, Single-Stranded