title: Comparison of epithelial and fibroblastic cell behavior on nano/micro-topographic PCL membranes produced by crystallinity control.
abstract: In this study, the relationship between the cellular morphology and the material surface topography was investigated. Poly(ε-caprolactone) (PCL) membranes were prepared in a wide range of surface wettabilities by means of crystallinity-controlled solvent casting process. Membrane surfaces were characterized by atomic force microscope (AFM), scanning electron microscope (SEM), and static/dynamic water contact angle measurements. It was found that solvent evaporation and non-solvent (methanol) addition to the solvent (THF) are the most decisive parameters to change the surface topography. The non-solvent addition and the decrease in solvent evaporation temperature from room temperature to -20 °C caused increased polymeric chain mobility and crystallization time. Such changes in crystallization parameters led to the formation of micro/nano-sized features on the membrane. Cell culture studies indicated that in contrast to Madin Darby kidney (MDBK) epithelial cells, L929 mouse fibroblast preferred rough and porous surfaces.
authors.affiliation: Hacettepe University, Chemical Eng. Dept., 06800 Beytepe, Ankara, Turkey. menemse@hacettepe.edu.tr
authors.forename: Menemşe
authors.initials: M
authors.lastname: Gümüşderelioğlu
authors.forename: F Betül
authors.initials: FB
authors.lastname: Kaya
authors.forename: Işıl Gerçek
authors.initials: IG
authors.lastname: Beşkardeş
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002460:Cell Line; D003460:Crystallization; D004285:Dogs; D004847:Epithelial Cells / Q000166:cytology*; D005347:Fibroblasts / Q000166:cytology*; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry*; D011091:Polyesters; D016062:Porosity; D012997:Solvents; D013499:Surface Properties
chemical_list: D011091:Polyesters; D012997:Solvents; C016240:polycaprolactone