title: Determination of aflatoxin B1 in cereals by homogeneous liquid-liquid extraction coupled to high performance liquid chromatography-fluorescence detection.
abstract: A simple and rapid method based on homogeneous liquid-liquid extraction coupled to HPLC with fluorescence detection was developed for the determination of aflatoxin B1 (AFB1) in the rice and grain samples after post-column derivatization. The proposed method eliminated the use of immunoaffinity columns for clean-up in the determination of AFB1. The parameters affecting recovery and preconcentration such as type and volume of organic solvent, volume ratio of water/methanol, concentration of phase separator reagent and extraction time were optimized. Under the optimized conditions, the calibration graph was linear in the concentration range of 0.01-1.0 ng/g with the detection limit of 0.003 ng/g. This method was successfully applied for the analysis of AFB1 in different cereal samples.
authors.affiliation: International Goods Inspection Company, Tehran, Iran. n.sheijooni@yahoo.com
authors.forename: Neda
authors.initials: N
authors.lastname: Sheijooni-Fumani
authors.forename: Jalal
authors.initials: J
authors.lastname: Hassan
authors.forename: Seyed R
authors.initials: SR
authors.lastname: Yousefi
mesh_terms: D016604:Aflatoxin B1 / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D002523:Edible Grain / Q000737:chemistry*; D005453:Fluorescence; D059625:Liquid-Liquid Extraction
chemical_list: D016604:Aflatoxin B1