title: Assessment of microbial growth on the surface of materials in contact with water intended for human consumption using ATP method.
abstract: Elaboration of an assessment method for plumbing materials contacting drinking water was the main purpose of this study. The investigation was conducted in 8 week cycles in dynamic conditions using a continuous flow reactor. Microbial growth was measured indirectly by a bioluminescence technique (ATP assay). Every week swabs from the surface of tested materials (polypropylene and different types of polyethylene), from the domestic market were collected and the level of bioluminescence was examined. The results obtained from the surface of tested materials were repeatable and clearly approximated those obtained from the surface of a negative control (stainless steel, low susceptibility for microbial growth). The level of bioluminescence (ATP) on the surface of positive control (paraffin, high susceptibility for microbial growth) was many times higher than that observed on other materials. The presented investigation was the main part of a validation process, which in short time will serve to initiate a complete assessment system for organic materials contacting drinking water.
authors.affiliation: Department of Municipal Hygiene, National Institute of Public Health, National Institute of Hygiene, Warsaw, Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Szczotko
authors.forename: Adam
authors.initials: A
authors.lastname: Krogulski
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis*; D000535:Aluminum; D018441:Biofilms / Q000254:growth & development*; D006801:Humans; D049409:Luciferases, Firefly / Q000378:metabolism; D049408:Luminescent Agents / Q000378:metabolism; D010232:Paraffin; D020959:Polyethylene; D011126:Polypropylenes; D013193:Stainless Steel; D013499:Surface Properties; D013997:Time Factors; D014871:Water Microbiology; D014881:Water Supply
chemical_list: D049408:Luminescent Agents; D011126:Polypropylenes; D013193:Stainless Steel; D010232:Paraffin; D000255:Adenosine Triphosphate; D020959:Polyethylene; D000535:Aluminum; D049409:Luciferases, Firefly