title: Functionalized micromachines for selective and rapid isolation of nucleic acid targets from complex samples.
abstract: The transport properties of single-strand DNA probe-modified self-propelling micromachines are exploited for "on-the-fly" hybridization and selective single-step isolation of target nucleic acids from "raw" microliter biological samples (serum, urine, crude E. coli lysate, saliva). The rapid movement of the guided modified microrockets induces fluid convection, which enhances the hybridization efficiency, thus enabling the rapid and selective isolation of nucleic acid targets from untreated samples. The integration of these autonomous microrockets into a lab-on-chip device that provides both nucleic acid isolation and downstream analysis could thus be attractive for diverse applications.
authors.affiliation: Department of Nanoengineering, University of California-San Diego, La Jolla, California 92093, United States.
authors.forename: Daniel
authors.initials: D
authors.lastname: Kagan
authors.forename: Susana
authors.initials: S
authors.lastname: Campuzano
authors.forename: Shankar
authors.initials: S
authors.lastname: Balasubramanian
authors.forename: Filiz
authors.initials: F
authors.lastname: Kuralay
authors.forename: Gerd-Uwe
authors.initials: GU
authors.lastname: Flechsig
authors.forename: Joseph
authors.initials: J
authors.lastname: Wang
mesh_terms: D015374:Biosensing Techniques; D004247:DNA / Q000737:chemistry*; D018076:DNA, Complementary / Q000378:metabolism; D016503:Drug Delivery Systems; D004926:Escherichia coli / Q000378:metabolism; D006801:Humans; D007477:Ions; D008856:Microscopy, Fluorescence / Q000379:methods; D009038:Motion; D009693:Nucleic Acid Hybridization; D009696:Nucleic Acids / Q000737:chemistry*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D044967:Serum / Q000378:metabolism; D013997:Time Factors; D014556:Urine
chemical_list: D018076:DNA, Complementary; D007477:Ions; D009696:Nucleic Acids; D004247:DNA