title: Anti-annexin V IgM and IgG autoantibodies and the risk of idiopathic recurrent spontaneous miscarriage.
abstract: Anti-annexin V antibodies have been identified as risk factors for recurrent spontaneous miscarriage (RSM) in some, but not all previous studies. We investigated the association between anti-annexin IgM and IgG in RSM cases and control women. Blood samples from 244 women with idiopathic RSM, and 283 multi-parous control women were tested for anti-annexin V antibodies by ELISA. A significant elevation in anti-annexin V IgM and IgG was seen in the RSM cases. An increased prevalence of elevated anti-annexin V IgM and to a lesser extent anti-annexin V IgG was seen in RSM patients. Receiver operating characteristic analysis indicated that the area under the curve for anti-annexin V IgM was 0.916, and for anti-annexin V IgG was 0.725. A systematic shift in anti-annexin V IgM and IgG distributions toward higher values occurred in RSM women, which was confirmed by percentile analysis. For each of the anti-annexin V isotypes, the adjusted odds ratio increased as the percentile value increased; the strongest risk was for anti-annexin V IgM, in which the 99th percentile (P99) was associated with a 165-fold higher risk than P50, and for anti-annexin V IgG where P99 was associated with a 38-fold higher risk than P50. In addition, a higher prevalence of elevated anti-annexin V IgM and anti-annexin V IgG was seen in RSM cases than in control women. We conclude that anti-annexin V IgM and IgG antibody positivity are independent risk factors for RSM.
authors.affiliation: Department of Medical Biochemistry, Arabian Gulf University, Manama, Bahrain.
authors.forename: Mai S
authors.initials: MS
authors.lastname: Sater
authors.forename: Ramzi R
authors.initials: RR
authors.lastname: Finan
authors.forename: Fekria E
authors.initials: FE
authors.lastname: Mustafa
authors.forename: Ghada M
authors.initials: GM
authors.lastname: Al-Khateeb
authors.forename: Wassim Y
authors.initials: WY
authors.lastname: Almawi
mesh_terms: D000026:Abortion, Habitual / Q000175:diagnosis* / Q000453:epidemiology / Q000276:immunology* / Q000503:physiopathology; D000328:Adult; D017304:Annexin A5 / Q000276:immunology*; D001323:Autoantibodies / Q000097:blood; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D007861:Lebanon; D011247:Pregnancy; D011379:Prognosis; D012307:Risk Factors
chemical_list: D017304:Annexin A5; D001323:Autoantibodies; D007074:Immunoglobulin G; D007075:Immunoglobulin M