title: The truncated phage lysin CHAP(k) eliminates Staphylococcus aureus in the nares of mice.
abstract: The endolysin LysK derived from staphylococcal phage K has previously been shown to have two enzymatic domains, one of which is an N-acetylmuramoyl-L-alanine amidase and the other a cysteine/histidine-dependant amidohydrolase/peptidase designated CHAP(k). The latter, when cloned as a single-domain truncated enzyme, is conveniently overexpressed in a highly-soluble form. This enzyme was shown to be highly active in vitro against live cell suspensions of S. aureus. In the current study, the IVIS imaging system was used to demonstrate the effective elimination of a lux labeled S. aureus from the nares of BALB/c mice.
authors.affiliation: Department of Biological Sciences, Cork Institute of Technology, Cork, Ireland.
authors.forename: Mark
authors.initials: M
authors.lastname: Fenton
authors.forename: Pat G
authors.initials: PG
authors.lastname: Casey
authors.forename: Colin
authors.initials: C
authors.lastname: Hill
authors.forename: Cormac Gm
authors.initials: CG
authors.lastname: Gahan
authors.forename: R Paul
authors.initials: RP
authors.lastname: Ross
authors.forename: Olivia
authors.initials: O
authors.lastname: McAuliffe
authors.forename: Jim
authors.initials: J
authors.lastname: O'Mahony
authors.forename: Fiona
authors.initials: F
authors.lastname: Maher
authors.forename: Aidan
authors.initials: A
authors.lastname: Coffey
mesh_terms: D000619:Aminohydrolases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001433:Bacteriolysis; D010450:Endopeptidases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D009297:Nasal Mucosa / Q000382:microbiology*; D010447:Peptide Hydrolases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D013204:Staphylococcus Phages / Q000201:enzymology*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000502:physiology
chemical_list: D000900:Anti-Bacterial Agents; D010450:Endopeptidases; D010447:Peptide Hydrolases; C001143:endolysin; D000619:Aminohydrolases