title: microRNA-145 suppresses lung adenocarcinoma-initiating cell proliferation by targeting OCT4.
abstract: Recent studies demonstrated that microRNA-145 is downregulated in human cancer cells and may function as a tumor suppressor. However, its role in lung cancer tumorigenesis remains unclear. Here, we demonstrated that upregulation of miR-145 reduced the proliferation and invasion as well as the ratio of CD133-positive initiating cells and the tumorosphere growth capacity of the human lung adenocarcinoma A549 cell line. The direct targeting of miR-145 to OCT4 mRNA was predicted by bioinformatic analysis and validated by a luciferase reporter system. We, therefore, confirmed that miR-145 can impair the proliferation of human lung adenocarcinoma-initiating cells by targeting OCT4 and leads to inhibition of lung cancer development.
authors.affiliation: Department of Thoracic Surgery, Nanjing Medical University Affiliated Cancer Hospital of Jiangsu Province, Cancer Institution of Jiangsu Province, Nanjing 210009, PR China.
authors.forename: Rong
authors.initials: R
authors.lastname: Yin
authors.forename: Shuai
authors.initials: S
authors.lastname: Zhang
authors.forename: Yaqin
authors.initials: Y
authors.lastname: Wu
authors.forename: Xiaohu
authors.initials: X
authors.lastname: Fan
authors.forename: Feng
authors.initials: F
authors.lastname: Jiang
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zhang
authors.forename: Dongjie
authors.initials: D
authors.lastname: Feng
authors.forename: Xirong
authors.initials: X
authors.lastname: Guo
authors.forename: Lin
authors.initials: L
authors.lastname: Xu
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D050814:Octamer Transcription Factor-3 / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
chemical_list: C541010:MIRN145 microRNA, human; D035683:MicroRNAs; D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human