title: Photobleaching kinetics of Verteporfin and Lemuteporfin in cells and optically trapped multilamellar vesicles using two-photon excitation.
abstract: Verteporfin and Lemuteporfin are compared to examine the effect of their functional groups and therefore the localization in two-photon excitation (TPE) photodynamic therapy (PDT). We used singlet oxygen-related photobleaching of the sensitizers to assess TPE-induced singlet oxygen generation in multilamellar vesicles (MLVs) and U343 glioma cells under a variety of conditions. It was found that Lemuteporfin photobleached at a faster rate than Verteporfin in the majority of environments. Also, Verteporfin and Lemuteporfin exhibited different behaviors when in hypoxic environments relative to those in oxygenated MLVs. These differences are attributed to the sensitizer location in the membrane and their relative mobilities throughout membranes and cells.
authors.affiliation: Department of Chemistry, University of Calgary, Calgary, AB, Canada.
authors.forename: Amy D
authors.initials: AD
authors.lastname: Tekrony
authors.forename: Nicole M
authors.initials: NM
authors.lastname: Kelly
authors.forename: B Alexander
authors.initials: BA
authors.lastname: Fage
authors.forename: David T
authors.initials: DT
authors.lastname: Cramb
mesh_terms: D045744:Cell Line, Tumor; D016543:Central Nervous System Neoplasms / Q000188:drug therapy / Q000473:pathology; D004058:Diffusion; D005026:Ethylene Glycols / Q000378:metabolism* / Q000494:pharmacology; D005909:Glioblastoma / Q000188:drug therapy / Q000473:pathology; D006801:Humans; D000860:Hypoxia; D007700:Kinetics; D008027:Light; D008567:Membranes, Artificial; D018613:Microscopy, Confocal; D010100:Oxygen / Q000378:metabolism*; D038761:Photobleaching / Q000528:radiation effects; D010778:Photochemotherapy / Q000379:methods*; D017785:Photons; D017319:Photosensitizing Agents / Q000378:metabolism* / Q000494:pharmacology; D011166:Porphyrins / Q000378:metabolism* / Q000494:pharmacology; D026082:Singlet Oxygen / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D000077362:Verteporfin
chemical_list: D005026:Ethylene Glycols; D008567:Membranes, Artificial; D017319:Photosensitizing Agents; D011166:Porphyrins; D000077362:Verteporfin; D026082:Singlet Oxygen; C510896:lemuteporfin; D010100:Oxygen