title: Efficient silencing of gene expression by an ASON-bulge-DNAzyme complex.
abstract: BACKGROUND
  DNAzymes are DNA molecules that can directly cleave cognate mRNA, and have been developed to silence gene expression for research and clinical purposes. The advantage of DNAzymes over ribozymes is that they are inexpensive to produce and exhibit good stability. The "10-23 DNA enzyme" is composed of a catalytic domain of 15 deoxynucleotides, flanked by two substrate-recognition domains of approximately eight nucleotides in each direction, which provides the complementary sequence required for specific binding to RNA substrates. However, these eight nucleotides might not afford sufficient binding energy to hold the RNA substrate along with the DNAzyme, which would interfere with the efficiency of the DNAzyme or cause side effects, such as the cleavage of non-cognate mRNAs.
  
  
  METHODS
  In this study, we inserted a nonpairing bulge at the 5' end of the "10-23 DNA enzyme" to enhance its efficiency and specificity. Different sizes of bulges were inserted at different positions in the 5' end of the DNAzyme. The non-matching bulge will avoid strong binding between the DNAzyme and target mRNA, which may interfere with the efficiency of the DNAzyme.
  
  
  CONCLUSIONS
  Our novel DNAzyme constructs could efficiently silence the expression of target genes, proving a powerful tool for gene silencing. The results showed that the six oligo bulge was the most effective when the six oligo bulge was 12-15 bp away from the core catalytic domain.
authors.affiliation: Institute of Animal Husbandry Veterinary Sciences, Shanghai Academy of Agricultural Sciences, Shanghai, China. yijianzhong@yahoo.com
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Yi
authors.forename: Chengqian
authors.initials: C
authors.lastname: Liu
mesh_terms: D001483:Base Sequence; D021881:DNA, Catalytic / Q000378:metabolism*; D015870:Gene Expression / Q000235:genetics; D020868:Gene Silencing / Q000502:physiology*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D009690:Nucleic Acid Conformation
chemical_list: D021881:DNA, Catalytic; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins