title: The overexpression in Arabidopsis thaliana of a Trichoderma harzianum gene that modulates glucosidase activity, and enhances tolerance to salt and osmotic stresses.
abstract: Using the TrichoEST database, generated in a previous functional genomics project from the beneficial filamentous fungus Trichoderma harzianum, a gene named Thkel1, which codes for a putative kelch-repeat protein, was isolated and characterized. Silencing of this gene in T. harzianum leads to a reduction of glucosidase activity and mycelial growth under abiotic stress conditions. Expression of this gene in Arabidopsis enhances plant tolerance to salt and osmotic stresses, accompanied by an increase in glucosidase activity and a reduction of abscisic acid levels compared to those observed in wild-type plants. Data presented throughout this article suggest the high value of T. harzianum as a source of genes able to facilitate the achievement of producing plants resistant to abiotic stresses without alteration of their phenotype.
authors.affiliation: Departamento de Microbiología y Genética, Centro Hispano-Luso de Investigaciones Agrarias (CIALE), Facultad de Farmacia, Universidad de Salamanca, C/Río Duero 12, Campus de Villamayor, 37185 Salamanca, Spain.
authors.forename: Rosa
authors.initials: R
authors.lastname: Hermosa
authors.forename: Leticia
authors.initials: L
authors.lastname: Botella
authors.forename: Emma
authors.initials: E
authors.lastname: Keck
authors.forename: Jesús Ángel
authors.initials: JÁ
authors.lastname: Jiménez
authors.forename: Marta
authors.initials: M
authors.lastname: Montero-Barrientos
authors.forename: Vicent
authors.initials: V
authors.lastname: Arbona
authors.forename: Aurelio
authors.initials: A
authors.lastname: Gómez-Cadenas
authors.forename: Enrique
authors.initials: E
authors.lastname: Monte
authors.forename: Carlos
authors.initials: C
authors.lastname: Nicolás
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism; D000222:Adaptation, Physiological; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D005800:Genes, Fungal; D005822:Genetic Vectors; D005959:Glucosidases / Q000235:genetics / Q000378:metabolism*; D009997:Osmotic Pressure; D010802:Phylogeny; D054046:Plant Stomata / Q000502:physiology; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D055051:Salt-Tolerant Plants / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D012965:Sodium Chloride / Q000378:metabolism; D014242:Trichoderma / Q000235:genetics*
chemical_list: D012965:Sodium Chloride; D000040:Abscisic Acid; D005959:Glucosidases