title: Immune response and protective efficacy against homologous challenge in BALB/c mice vaccinated with DNA vaccine encoding Toxoplasma gondii actin depolymerizing factor gene.
abstract: A DNA vaccine (pVAX1-TgADF) encoding Toxoplasma gondii actin depolymerizing factor (ADF) gene was constructed and the immune response and protective efficacy of this vaccine against homologous challenge in BALB/c mice were evaluated. High titers of specific antibody and increases in the percentage of CD4(+) and CD8(+) T lymphocyte cells were observed from BALB/c mice vaccinated with pVAX1-TgADF (P<0.05), when PBS group was used as control. The survival time of BALB/c mice in pVAX1-TgADF group was longer than those in control groups. The numbers of brain cysts in the experimental BALB/c mice immunized with pVAX1-TgADF reduced significantly compared with those in PBS group (P<0.05), and the rate of reduction could reach to around 42.8%. These results suggested that the DNA vaccine pVAX1-TgADF could generate specific humoral and cellular immune responses, prolong survival times, and reduce brain cysts load against T. gondii infection in BALB/c mice.
authors.affiliation: College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun, Jilin 130062, China. Jianhuali7207@163.com
authors.forename: Jianhua
authors.initials: J
authors.lastname: Li
authors.forename: Xiangsheng
authors.initials: X
authors.lastname: Huang
authors.forename: Guocai
authors.initials: G
authors.lastname: Zhang
authors.forename: Pengtao
authors.initials: P
authors.lastname: Gong
authors.forename: Xichen
authors.initials: X
authors.lastname: Zhang
authors.forename: Ling
authors.initials: L
authors.lastname: Wu
mesh_terms: D051339:Actin Depolymerizing Factors / Q000235:genetics / Q000276:immunology*; D000818:Animals; D005260:Female; D006367:HeLa Cells; D006801:Humans; D007111:Immunity, Cellular; D056724:Immunity, Humoral; D051379:Mice; D008807:Mice, Inbred BALB C; D016052:Protozoan Vaccines / Q000276:immunology*; D014122:Toxoplasma / Q000235:genetics* / Q000276:immunology*; D014124:Toxoplasmosis, Animal / Q000517:prevention & control*; D019444:Vaccines, DNA / Q000276:immunology
chemical_list: D051339:Actin Depolymerizing Factors; D016052:Protozoan Vaccines; D019444:Vaccines, DNA