title: Evaluation of oxidative stress tolerance in maize (Zea mays L.) seedlings in response to drought.
abstract: Seedlings of selected six genotypes of maize (Zea mays L.) differing in their drought sensitivity (LM5 and Parkash drought-tolerant and PMH2, JH3459, Paras and LM14 as drought-sensitive) were exposed to 72 h drought stress at two leaf stage. Alterations in their antioxidant pools combined with activities of enzymes involved in defense against oxidative stress were investigated in leaves. Activities of some reactive oxygen species (ROS)-scavenging enzymes, catalase (CAT) and ascorbate peroxidase (APX) were enhanced in tolerant genotypes in response to drought stress. Superoxide dismutase (SOD) activity was significantly decreased in sensitive genotypes, but remained unchanged in tolerant genotypes under stress. Peroxidase (POX) activity was significantly induced in tolerant, as well as sensitive genotypes. Imposition of stress led to increase in H2O2 and malondialdehyde (MDA, a marker for lipid peroxidation) content in sensitive genotypes, while in tolerant genotypes no change was observed. Significant increase in glutathione content was observed in sensitive genotypes. Ascorbic acid pool was induced in both tolerant and sensitive genotypes, but induction was more pronounced in tolerant genotypes. Significant activation of antioxidative defence mechanisms correlated with drought-induced oxidative stress tolerance was the characteristic of the drought tolerant genotypes. These studies provide a mechanism for drought tolerance in maize seedlings.
authors.affiliation: Department of Biochemistry, Punjab Agricultural University, Ludhiana 141004, Punjab, India.
authors.forename: Vishal
authors.initials: V
authors.lastname: Chugh
authors.forename: Narinder
authors.initials: N
authors.lastname: Kaur
authors.forename: Anil K
authors.initials: AK
authors.lastname: Gupta
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D060387:Ascorbate Peroxidases; D001205:Ascorbic Acid / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D055864:Droughts; D005838:Genotype; D005980:Glutathione Reductase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D015227:Lipid Peroxidation; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress; D010544:Peroxidases / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D036226:Seedlings / Q000254:growth & development / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism; D003313:Zea mays / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D008315:Malondialdehyde; D006861:Hydrogen Peroxide; D010544:Peroxidases; D060387:Ascorbate Peroxidases; D002374:Catalase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D001205:Ascorbic Acid