title: ATP requirement for acidic resistance in Escherichia coli.
abstract: ATP participates in many cellular metabolic processes as a major substrate to supply energy. Many systems for acidic resistance (AR) under extremely acidic conditions have been reported, but the role of ATP has not been examined. To clarify whether or not ATP is necessary for the AR in Escherichia coli, the AR of mutants deficient in genes for ATP biosynthesis was investigated in this study. The deletion of purA or purB, each of which encodes enzymes to produce AMP from inosinate (IMP), markedly decreased the AR. The content of ATP in these mutants decreased rapidly at pH 2.5 compared to that of the wild type. The AR was again decreased significantly by the mutation of adk, which encoded an enzyme to produce ADP from AMP. The DNA damage in the purA and purB mutants was higher than that in the wild type. These results demonstrated that metabolic processes that require ATP participate in survival under extremely acidic conditions, and that one such system is the ATP-dependent DNA repair system.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Chiba University, 1-8-1, Inohana, Chuo-ku, Chiba 260-8675, Japan.
authors.forename: Yirong
authors.initials: Y
authors.lastname: Sun
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Fukamachi
authors.forename: Hiromi
authors.initials: H
authors.lastname: Saito
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kobayashi
mesh_terms: D000143:Acids / Q000494:pharmacology*; D000255:Adenosine Triphosphate / Q000378:metabolism*; D001120:Arginine / Q000378:metabolism / Q000494:pharmacology; D004249:DNA Damage; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D016680:Genome, Bacterial; D018698:Glutamic Acid / Q000378:metabolism / Q000494:pharmacology; D006706:Homeostasis; D006863:Hydrogen-Ion Concentration; D008239:Lysine / Q000378:metabolism / Q000494:pharmacology
chemical_list: D000143:Acids; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D018698:Glutamic Acid; D000255:Adenosine Triphosphate; D001120:Arginine; D008239:Lysine