title: Asymmetric dimethylarginine in children with homocystinuria or phenylketonuria.
abstract: Plasma concentration of asymmetric dimethylarginine (ADMA), an endogenous inhibitor of nitric oxide (NO) synthesis from L-arginine and a cardiovascular risk factor, was found to be elevated in plasma of homocysteinemic adults. Enhanced cardiovascular risk due to homocystinuria and impaired renal function has been found in patients with phenylketonuria (PKU) on protein-restricted diet. However, it is still unknown whether ADMA synthesis is also elevated in children with homocystinuria due to cystathionine beta-synthase deficiency (classical homocystinuria), and whether ADMA may play a role in phenylketonuria in childhood. In the present study, we investigated the status of the L-arginine/NO pathway in six young patients with homocystinuria, in 52 young phenylketonuria patients on natural protein-restricted diet, and in age- and gender-matched healthy children serving as controls. ADMA in plasma and urine was determined by GC-MS/MS. The NO metabolites nitrate and nitrite in plasma and urine, and urinary dimethylamine (DMA), the dimethylarginine dimethylaminohydrolase (DDAH) metabolite of ADMA, were measured by GC-MS. Unlike urine ADMA excretion, plasma ADMA concentration in patients with homocystinuria was significantly higher than in controls (660±158 vs. 475±77 nM, P=0.035). DMA excretion rate was considerably higher in children with homocystinuria as compared to controls (62.2±24.5 vs. 6.5±2.9 μmol/mmol creatinine, P=0.068), indicating enhanced DDAH activity in this disease. In contrast and unexpectedly, phenylketonuria patients had significantly lower ADMA plasma concentrations compared to controls (512±136 vs. 585±125 nM, P=0.009). Phenylketonuria patients and controls had similar L-arginine/ADMA molar ratios in plasma. Urinary nitrite excretion was significantly higher in phenylketonuria as compared to healthy controls (1.7±1.7 vs. 0.7±1.2 μmol/mmol creatinine, P=0.003). Our study shows that the L-arginine/NO pathway is differently altered in children with phenylketonuria and homocystinuria. Analogous to hyperhomocysteinemic adults, elevated ADMA plasma concentrations could be a cardiovascular risk factor in children with homocystinuria. In phenylketonuria, the L-arginine/NO pathway seems not be altered. Delineation of the role of ADMA in childhood phenylketonuria and homocystinuria demands further investigation.
authors.affiliation: Department of Pediatrics, Medical School Hannover, Carl-Neuberg Str. 1, 30625, Hannover, Germany. kanzelmeyer.nele@mh-hannover.de
authors.forename: Nele
authors.initials: N
authors.lastname: Kanzelmeyer
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Tsikas
authors.forename: Kristine
authors.initials: K
authors.lastname: Chobanyan-Jürgens
authors.forename: Bibiana
authors.initials: B
authors.lastname: Beckmann
authors.forename: Bernhard
authors.initials: B
authors.lastname: Vaske
authors.forename: Sabine
authors.initials: S
authors.lastname: Illsinger
authors.forename: Anibh M
authors.initials: AM
authors.lastname: Das
authors.forename: Thomas
authors.initials: T
authors.lastname: Lücke
mesh_terms: D000293:Adolescent; D000581:Amidohydrolases / Q000097:blood / Q000652:urine; D001120:Arginine / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000097:blood / Q000652:urine; D002318:Cardiovascular Diseases / Q000209:etiology; D002648:Child; D002675:Child, Preschool; D004123:Dimethylamines / Q000097:blood / Q000652:urine; D006712:Homocystinuria / Q000097:blood / Q000150:complications / Q000652:urine; D006801:Humans; D053858:Metabolic Networks and Pathways; D009569:Nitric Oxide / Q000097:blood* / Q000652:urine*; D010661:Phenylketonurias / Q000097:blood / Q000150:complications / Q000652:urine; D012307:Risk Factors; D055815:Young Adult
chemical_list: D004123:Dimethylamines; C487735:dimethylarginine; D009569:Nitric Oxide; D001120:Arginine; C034516:dimethylamine; D000581:Amidohydrolases; C059999:dimethylargininase