title: DNA origami with complex curvatures in three-dimensional space.
abstract: We present a strategy to design and construct self-assembling DNA nanostructures that define intricate curved surfaces in three-dimensional (3D) space using the DNA origami folding technique. Double-helical DNA is bent to follow the rounded contours of the target object, and potential strand crossovers are subsequently identified. Concentric rings of DNA are used to generate in-plane curvature, constrained to 2D by rationally designed geometries and crossover networks. Out-of-plane curvature is introduced by adjusting the particular position and pattern of crossovers between adjacent DNA double helices, whose conformation often deviates from the natural, B-form twist density. A series of DNA nanostructures with high curvature--such as 2D arrangements of concentric rings and 3D spherical shells, ellipsoidal shells, and a nanoflask--were assembled.
authors.affiliation: The Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA. dongran.han@asu.edu
authors.forename: Dongran
authors.initials: D
authors.lastname: Han
authors.forename: Suchetan
authors.initials: S
authors.lastname: Pal
authors.forename: Jeanette
authors.initials: J
authors.lastname: Nangreave
authors.forename: Zhengtao
authors.initials: Z
authors.lastname: Deng
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Hao
authors.initials: H
authors.lastname: Yan
mesh_terms: D004247:DNA / Q000737:chemistry*; D008958:Models, Molecular; D049329:Nanostructures; D036103:Nanotechnology; D009690:Nucleic Acid Conformation
chemical_list: D004247:DNA