title: Linearizer gene circuits with negative feedback regulation.
abstract: Gene functional studies consist of phenotyping cells with altered gene expression. Improving the precision of current gene expression control techniques would enable more detailed studies of gene function. Here, we provide protocols for building synthetic gene constructs for tuning the expression of a gene in all the cells of a population precisely and uniformly, achieving expression levels proportional to the extracellular inducer concentration.
authors.affiliation: UT M. D. Anderson Cancer Center, Houston, TX, USA.
authors.forename: Dmitry
authors.initials: D
authors.lastname: Nevozhay
authors.forename: Rhys M
authors.initials: RM
authors.lastname: Adams
authors.forename: Gábor
authors.initials: G
authors.lastname: Balázsi
mesh_terms: D025461:Feedback, Physiological; D005434:Flow Cytometry; D005453:Fluorescence; D053263:Gene Regulatory Networks / Q000235:genetics*; D005800:Genes, Fungal / Q000235:genetics*; D017930:Genes, Reporter / Q000235:genetics; D005813:Genes, Synthetic / Q000235:genetics; D005819:Genetic Markers; D005821:Genetic Techniques; D008954:Models, Biological; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics*
chemical_list: D005819:Genetic Markers