title: A fluorescent microsphere-based method for assay of multiple analytes in plasma.
abstract: Measurement of multiple analytes can provide increased sensitivity and specificity for the detection and management of disease. The enzyme-linked immunosorbent assay (ELISA) is currently the "gold standard" for protein quantification; however, individual assays for each analyte must be performed, placing demand on sample volume. On the contrary, multiplex assays using microsphere-based technologies allow for multiple analytes to be simultaneously assayed within a single sample. Here, we present a protocol for the preparation and development of a multiple-analyte assay in human plasma using the BioPlex 200 platform (Bio-Rad), which incorporates xMAP technology (Luminex).
authors.affiliation: Ludwig Institute for Cancer Research, Royal Melbourne Hospital, Parkville, Australia.
authors.forename: Oliver K
authors.initials: OK
authors.lastname: Bernhard
authors.forename: Rommel A
authors.initials: RA
authors.lastname: Mathias
authors.forename: Thomas W
authors.initials: TW
authors.lastname: Barnes
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Simpson
mesh_terms: D000906:Antibodies; D001681:Biological Assay / Q000379:methods*; D002138:Calibration; D005456:Fluorescent Dyes / Q000378:metabolism*; D006801:Humans; D008863:Microspheres; D010949:Plasma / Q000737:chemistry*; D012015:Reference Standards; D013223:Statistics as Topic
chemical_list: D000906:Antibodies; D005456:Fluorescent Dyes