title: Assessment of cell proliferation with resazurin-based fluorescent dye.
abstract: The Alamar Blue assay is based on enzymatic reduction of indicator dye by viable cells and serves as an effective tool for assessing cell proliferation and as a screening technique. It can be applied in studies concentrating on animal, plant, yeast, and bacteria cells. Among the various methods for cell viability and cytotoxicity, it utilises all features of ideal and reliable test; it is one-step, sensitive, safe, non-toxic for cells, and cost-effective.
authors.affiliation: AO Research Institute, Davos Platz, Switzerland. ewa.czekanska@aofoundation.org
authors.forename: Ewa M
authors.initials: EM
authors.lastname: Czekanska
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002460:Cell Line; D049109:Cell Proliferation; D003584:Cytological Techniques / Q000379:methods*; D005456:Fluorescent Dyes / Q000378:metabolism*; D051379:Mice; D010078:Oxazines / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction; D012015:Reference Standards; D014966:Xanthenes / Q000737:chemistry / Q000378:metabolism*
chemical_list: D005456:Fluorescent Dyes; D010078:Oxazines; D014966:Xanthenes; C005843:resazurin; C014180:resorufin