title: Induction of aromatic ring: cleavage dioxygenases in Stenotrophomonas maltophilia strain KB2 in cometabolic systems.
abstract: Stenotrophomonas maltophilia KB2 is known to produce different enzymes of dioxygenase family. The aim of our studies was to determine activity of these enzymes after induction by benzoic acids in cometabolic systems with nitrophenols. We have shown that under cometabolic conditions KB2 strain degraded 0.25-0.4 mM of nitrophenols after 14 days of incubation. Simultaneously degradation of 3 mM of growth substrate during 1-3 days was observed depending on substrate as well as cometabolite used. From cometabolic systems with nitrophenols as cometabolites and 3,4-dihydroxybenzoate as a growth substrate, dioxygenases with the highest activity of protocatechuate 3,4-dioxygenase were isolated. Activity of catechol 1,2- dioxygenase and protocatechuate 4,5-dioxygenase was not observed. Catechol 2,3-dioxygenase was active only in cultures with 4-nitrophenol. Ability of KB2 strain to induce and synthesize various dioxygenases depending on substrate present in medium makes this strain useful in bioremediation of sites contaminated with different aromatic compounds.
authors.affiliation: Faculty of Biology and Environment Protection, Department of Biochemistry, University of Silesia, Jagiellonska 28, 40-032 Katowice, Poland.
authors.forename: Danuta
authors.initials: D
authors.lastname: Wojcieszyńska
authors.forename: Urszula
authors.initials: U
authors.lastname: Guzik
authors.forename: Izabela
authors.initials: I
authors.lastname: Greń
authors.forename: Magdalena
authors.initials: M
authors.lastname: Perkosz
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Hupert-Kocurek