title: Targeted imaging of colorectal dysplasia in living mice with fluorescence microendoscopy.
abstract: We validate specific binding activity of a fluorescence-labeled peptide to colorectal dysplasia in living mice using a miniature, flexible, fiber microendoscope that passes through the instrument channel of an endoscope. The microendoscope delivers excitation light at 473 nm through a fiber-optic bundle with outer diameter of 680 µm to collect en face images at 10 Hz with 4 µm lateral resolution. We applied the FITC-labeled peptide QPIHPNNM topically to colonic mucosa in genetically engineered mice that spontaneously develop adenomas. More than two-fold greater fluorescence intensity was measured from adenomas compared to adjacent normal-appearing mucosa. Images of adenomas showed irregular morphology characteristic of dysplasia.
authors.forename: Sakib F
authors.initials: SF
authors.lastname: Elahi
authors.forename: Sharon J
authors.initials: SJ
authors.lastname: Miller
authors.forename: Bishnu
authors.initials: B
authors.lastname: Joshi
authors.forename: Thomas D
authors.initials: TD
authors.lastname: Wang