title: Chemically defined conditions for human iPSC derivation and culture.
abstract: We re-examine the individual components for human embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) culture and formulate a cell culture system in which all protein reagents for liquid media, attachment surfaces and splitting are chemically defined. A major improvement is the lack of a serum albumin component, as variations in either animal- or human-sourced albumin batches have previously plagued human ESC and iPSC culture with inconsistencies. Using this new medium (E8) and vitronectin-coated surfaces, we demonstrate improved derivation efficiencies of vector-free human iPSCs with an episomal approach. This simplified E8 medium should facilitate both the research use and clinical applications of human ESCs and iPSCs and their derivatives, and should be applicable to other reprogramming methods.
authors.affiliation: Morgridge Institute for Research, Madison, Wisconsin, USA.
authors.forename: Guokai
authors.initials: G
authors.lastname: Chen
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Gulbranson
authors.forename: Zhonggang
authors.initials: Z
authors.lastname: Hou
authors.forename: Jennifer M
authors.initials: JM
authors.lastname: Bolin
authors.forename: Victor
authors.initials: V
authors.lastname: Ruotti
authors.forename: Mitchell D
authors.initials: MD
authors.lastname: Probasco
authors.forename: Kimberly
authors.initials: K
authors.lastname: Smuga-Otto
authors.forename: Sara E
authors.initials: SE
authors.lastname: Howden
authors.forename: Nicole R
authors.initials: NR
authors.lastname: Diol
authors.forename: Nicholas E
authors.initials: NE
authors.lastname: Propson
authors.forename: Ryan
authors.initials: R
authors.lastname: Wagner
authors.forename: Garrett O
authors.initials: GO
authors.lastname: Lee
authors.forename: Jessica
authors.initials: J
authors.lastname: Antosiewicz-Bourget
authors.forename: Joyce M C
authors.initials: JM
authors.lastname: Teng
authors.forename: James A
authors.initials: JA
authors.lastname: Thomson
mesh_terms: D000818:Animals; D001706:Biopsy; D002417:Cattle; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D002470:Cell Survival; D020099:Coated Materials, Biocompatible; D003470:Culture Media / Q000737:chemistry*; D016895:Culture Media, Serum-Free / Q000737:chemistry; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology; D015870:Gene Expression; D006133:Growth Substances; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D007621:Karyotyping; D012710:Serum Albumin, Bovine; D012867:Skin / Q000166:cytology; D019096:Vitronectin
chemical_list: D020099:Coated Materials, Biocompatible; D003470:Culture Media; D016895:Culture Media, Serum-Free; D006133:Growth Substances; D019096:Vitronectin; D012710:Serum Albumin, Bovine