title: The gold-nanoparticle-based surface plasmon resonance light scattering and visual DNA aptasensor for lysozyme.
abstract: We developed a new simple and sensitive assay for lysozyme based on gold nanoparticle plasmon resonance light scattering (PRLS) measurement and naked-eye detection using for the first time the lysozyme DNA aptamer as the recognition element. Lysozyme DNA aptamer could stabilize gold nanoparticles (AuNPs) at high ionic strength. Introducing lysozyme to the system easily triggered the aggregation of AuNPs, producing a red-to-blue color change of the solution, red-shifted plasmon absorption, and enhanced plasmon resonance light scattering. The linear range was found to be 0.2∼4 nM for 0.7 nM AuNPs, 0.3∼6 nM for 1.4 nM AuNPs and 0.6∼8 nM for 2.1 nM AuNPs. About 0.1 nM lysozyme can produce an observable enhancement of PRLS signal. For visual detection, 1 nM lysozyme can produce a very distinctive color change. Satisfactory recoveries were obtained for simulated saliva and diluted urine samples, indicating that the method has potential for analyses of clinical samples. The simplicity and high sensitivity that are consistent with the resources and needs of many laboratories makes this method a good choice for routine analysis.
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China.
authors.forename: Xinyi
authors.initials: X
authors.lastname: Wang
authors.forename: Yao
authors.initials: Y
authors.lastname: Xu
authors.forename: Yang
authors.initials: Y
authors.lastname: Chen
authors.forename: Limei
authors.initials: L
authors.lastname: Li
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.forename: Na
authors.initials: N
authors.lastname: Li
mesh_terms: D001483:Base Sequence; D015374:Biosensing Techniques; D004247:DNA / Q000235:genetics*; D017931:DNA Primers; D006046:Gold / Q000737:chemistry*; D008027:Light; D053768:Metal Nanoparticles; D009113:Muramidase / Q000378:metabolism*; D012542:Scattering, Radiation; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D017931:DNA Primers; D006046:Gold; D004247:DNA; D009113:Muramidase