title: Preparation and characterization of monoclonal antibody against translationally controlled tumor protein.
abstract: From our previous proteomic research, we found that translationally controlled tumor protein (TCTP) might play at least a partial role in colon adenocarcinoma progression. However, the precise impact of TCTP on colorectal cancer metastasis progression is currently still unknown. Therefore, immunology reagents are urgently needed to proceed with the next mechanism-related research. Moreover, the identification of TCTP expression level in tissue of colorectal cancer patients also requires substantial amounts of immunology reagents. In this report, monoclonal antibodies (MAbs) against to TCTP were made from hyperimmune Balb/c mice, by injecting 50 μg of purified antigen intraperitoneally. Hybridomas were screened by indirect enzyme-linked immunosorbent assay (ELISA) using purified protein. Finally six mouse hybridomas producing MAbs to TCTP were established. The MAbs obtained were fully characterized using Western blot analysis and immunohistochemistry. The results showed that these antibodies could be used for the preliminary application of the next mechanism-related research and TCTP expression level analysis.
authors.affiliation: The Institute of Antibody Engineering, School of Biotechnology, Southern Medical University, North Guangzhou Road, Baiyun, Guangzhou, China.
authors.forename: Ma
authors.initials: M
authors.lastname: Qiang
authors.forename: Wu
authors.initials: W
authors.lastname: Fenfang
authors.forename: Geng
authors.initials: G
authors.lastname: Yan
authors.forename: Shu
authors.initials: S
authors.lastname: Wen
authors.forename: Huang
authors.initials: H
authors.lastname: Maoliang
authors.forename: Wu
authors.initials: W
authors.lastname: Yingsong
authors.forename: Xu
authors.initials: X
authors.lastname: Weiwen
authors.forename: Li
authors.initials: L
authors.lastname: Ming
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D014408:Biomarkers, Tumor / Q000235:genetics / Q000276:immunology*; D015153:Blotting, Western; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D006825:Hybridomas / Q000276:immunology*; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D000090324:Tumor Protein, Translationally-Controlled 1
chemical_list: D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D017931:DNA Primers; C000715648:Tpt1 protein, mouse; D000090324:Tumor Protein, Translationally-Controlled 1