title: Novel polymer coupled lipid nanoparticle of paclitaxel with synergistic enhanced efficacy against cancer.
abstract: This study was aimed to develop chitosan coupled lipid nanoparticle (Ch-Ptx-Lip), where immunomodulatory activity of chitosan and chemotherapeutic effect of paclitaxel can synergistically enhance efficacy of this novel drug delivery system. Particle size, zeta potential and % Entrapment Efficiency (%EE) of Ch-Ptx-Lip was found to be 113.3 +/- 4.5 nm (PDI: 0.38), (+)35.7 +/- 2.9 mV and 96.4 +/- 1.8% respectively. Morphological evaluation of these particles by TEM shows circular bilayer structure along with outer polymer layers. Ch-Ptx-Lip showed significantly enhanced cytotoxicity (p < 0.05) compared with marketed formulation (Ptx Solution) and Ptx-Lip when evaluated in-vitro by MTT assay on MCF-7 cell lines. Improved efficacy with lower dose of drug can be a new strategy against cancer with minimum side effects and shorter treatment duration.
authors.affiliation: Pharmaceutics Division, Central Drug Research Institute (CSIR), Lucknow 226001, India.
authors.forename: D
authors.initials: D
authors.lastname: Singodia
authors.forename: S
authors.initials: S
authors.lastname: Talegaonkar
authors.forename: R K
authors.initials: RK
authors.lastname: Khar
authors.forename: P R
authors.initials: PR
authors.lastname: Mishra
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000737:chemistry; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000473:pathology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D053769:Nanocapsules / Q000008:administration & dosage / Q000737:chemistry*; D017239:Paclitaxel / Q000008:administration & dosage* / Q000737:chemistry; D016896:Treatment Outcome
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D008055:Lipids; D053769:Nanocapsules; D048271:Chitosan; D017239:Paclitaxel