title: Development of novel microsatellite markers to identify the different invasive lineages in the Corbicula complex and to assess androgenesis.
abstract: Reliable markers are needed to identify the lineages in the invasive clam genus Corbicula. Previous studies have demonstrated that mitochondrial (mt) DNA poorly resolves Corbicula phylogeny, owing to its androgenetic reproductive mode. Moreover, hybridization and mitochondrial/nuclear mismatches occur. We developed the first eleven polymorphic markers to detect these phenomena and to investigate the nuclear identity of Corbicula populations. These microsatellite loci revealed three main lineages in Western Europe. One locus allowed rapid discrimination of these three lineages on agarose gel, saving time and money. Moreover, the eleven markers were successfully cross-amplified in the invasive Corbicula lineages found in North America.
authors.affiliation: Unit of Research in Organismal Biology (URBO), University of Namur, Rue de Bruxelles, 61, 5000 Namur, Belgium. lise-marie.pigneur@fundp.ac.be
authors.forename: L-M
authors.initials: LM
authors.lastname: Pigneur
authors.forename: A-M
authors.initials: AM
authors.lastname: Risterucci
authors.forename: N
authors.initials: N
authors.lastname: Dauchot
authors.forename: X
authors.initials: X
authors.lastname: Li
authors.forename: K
authors.initials: K
authors.lastname: Van Doninck
mesh_terms: D000818:Animals; D049874:Corbicula / Q000145:classification* / Q000235:genetics*; D005060:Europe; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000191:economics / Q000379:methods*; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D046468:Sexual Development; D013997:Time Factors