title: Genotoxicity of crude extracts of cyanobacteria from Taihu Lake on carp (Cyprinus carpio).
abstract: Genotoxicity of crude cyanobacteria extracts (CBE) from blooms in Taihu Lake, China toward common carp (Cyprinus carpio) was measured. The primary extracellular product was determined by HPLC to be Microcystin-LR (MC-LR, L for leucine and R for arginine) with an average concentration of 2.4 × 10(2) μg MC g(-1) dry weight of cyanobacteria. Acute toxicity to carp, expressed as the 72-h LC(50,) was 53 mg, dw cyanobacteria L(-1). Genotoxicity, as determined by the micronucleus (MN) and comet assays were both dose- and time-depended. Deformities of cellular organelles in liver and gill were observed by use of transmission electron microscopy (TEM). The results showed that MC-LR from cyanobacteria from Taihu Lake could induce genotoxic response and tissue-level morphological changes in common carp.
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Xianlin Campus, 163 Xianlin Avenue, Nanjing, 210046, People's Republic of China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Wu
authors.forename: Mei
authors.initials: M
authors.lastname: Li
authors.forename: Xiangyu
authors.initials: X
authors.lastname: Gao
authors.forename: John P
authors.initials: JP
authors.lastname: Giesy
authors.forename: Yibin
authors.initials: Y
authors.lastname: Cui
authors.forename: Liuyan
authors.initials: L
authors.lastname: Yang
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Kong
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000633:toxicity*; D002347:Carps / Q000502:physiology*; D002681:China; D000458:Cyanobacteria / Q000472:pathogenicity*; D000087522:Cyanobacteria Toxins; D005880:Gills / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure; D057097:Harmful Algal Bloom; D008099:Liver / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure; D008387:Marine Toxins / Q000633:toxicity*; D052998:Microcystins / Q000633:toxicity*; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*
chemical_list: D001427:Bacterial Toxins; D000087522:Cyanobacteria Toxins; D008387:Marine Toxins; D052998:Microcystins; D009153:Mutagens