title: Reduced faecal shedding of Escherichia coli O157:H7 in cattle following systemic vaccination with γ-intimin C₂₈₀ and EspB proteins.
abstract: Enterohaemorrhagic Escherichia coli (EHEC) O157:H7 is the most prevalent EHEC serotype that has been recovered from patients with haemolytic uremic syndrome (HUS) worldwide. Vaccination of cattle, the main reservoir of EHEC O157:H7, could be a logical strategy to fight infection in humans. This study evaluated a vaccine based on the carboxyl-terminal fragment of 280 amino acids of γ-intimin (γ-intimin C₂₈₀) and EspB, two key colonization factors of E. coli O157:H7. Intramuscular immunization elicited significantly high levels of serum IgG antibodies against both proteins. Antigen-specific IgA and IgG were also induced in saliva, but only the IgA response was significant. Following experimental challenge with E. coli O157:H7, a significant reduction in bacterial shedding was observed in vaccinated calves, compared to control group. These promising results suggest that systemic immunization of cattle with intimin and EspB could be a feasible strategy to reduce EHEC O157:H7 faecal shedding in cattle.
authors.affiliation: Instituto de Patobiología, Instituto Nacional de Tecnología Agropecuaria (INTA), Los Reseros y Las Cabañas, 1712 Castelar, Argentina.
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Vilte
authors.forename: Mariano
authors.initials: M
authors.lastname: Larzábal
authors.forename: Sergio
authors.initials: S
authors.lastname: Garbaccio
authors.forename: Mariela
authors.initials: M
authors.lastname: Gammella
authors.forename: Bettina C
authors.initials: BC
authors.lastname: Rabinovitz
authors.forename: Ana M
authors.initials: AM
authors.lastname: Elizondo
authors.forename: Rodolfo J C
authors.initials: RJ
authors.lastname: Cantet
authors.forename: Fernando
authors.initials: F
authors.lastname: Delgado
authors.forename: Virginia
authors.initials: V
authors.lastname: Meikle
authors.forename: Angel
authors.initials: A
authors.lastname: Cataldi
authors.forename: Elsa C
authors.initials: EC
authors.lastname: Mercado
mesh_terms: D018829:Adhesins, Bacterial / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D057138:Bacterial Shedding / Q000502:physiology*; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D004927:Escherichia coli Infections / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary; D019453:Escherichia coli O157 / Q000276:immunology* / Q000472:pathogenicity; D029968:Escherichia coli Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D022361:Escherichia coli Vaccines / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology; D005243:Feces / Q000382:microbiology*; D007273:Injections, Intramuscular; D008297:Male; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D016896:Treatment Outcome; D014611:Vaccination
chemical_list: D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; C087863:EaeB protein, E coli; D029968:Escherichia coli Proteins; D022361:Escherichia coli Vaccines; D011994:Recombinant Proteins; C073819:eaeA protein, E coli