title: Using latent class analysis to estimate the test characteristics of the γ-interferon test, the single intradermal comparative tuberculin test and a multiplex immunoassay under Irish conditions.
abstract: Considerable effort has been devoted to improving the existing diagnostic tests for bovine tuberculosis (single intradermal comparative tuberculin test [SICTT] and γ-interferon assay [γ-IFN]) and to develop new tests. Previously, the diagnostic characteristics (sensitivity, specificity) have been estimated in populations with defined infection status. However, these approaches can be problematic as there may be few herds in Ireland where freedom from infection is guaranteed. We used latent class models to estimate the diagnostic characteristics of existing (SICTT and γ-IFN) and new (multiplex immunoassay [Enferplex-TB]) diagnostic tests under Irish field conditions where true disease status was unknown. The study population consisted of herds recruited in areas with no known TB problems (2197 animals) and herds experiencing a confirmed TB breakdown (2740 animals). A Bayesian model was developed, allowing for dependence between SICTT and γ-IFN, while assuming independence from the Enferplex-TB test. Different test interpretations were used for the analysis: SICTT (standard and severe interpretation), γ-IFN (a single interpretation), and a range of interpretations for the Enferplex-TB (level-1 [high sensitivity interpretation] to level-5 [high specificity interpretation]). The sensitivity and specificity (95% posterior credibility intervals; 95% PCI) of SICTT[standard] relative to Enferplex-TB[level-1] and γ-IFN were 52.9-60.8% and 99.2-99.8%, respectively. Equivalent estimates for γ-IFN relative to Enferplex-TB[level-1] and SICTT were 63.1-70.1% and 86.8-89.4%, respectively. Sensitivity of Enferplex-TB[level-1] (95% PCI: 64.8-71.9%) was superior to the SICTT[standard], and specificity of the Enferplex-TB[level-5] was superior to γ-IFN (95% PCI: 99.6-100.0%). These results provide robust measures of sensitivity and specificity under field conditions in Ireland and suggest that the Enferplex-TB test has the potential to improve on current diagnostics for TB infection in cattle. The extent of that potential will be assessed in further studies.
authors.affiliation: Centre for Veterinary Epidemiology and Risk Analysis, Veterinary Sciences Centre, School of Agriculture, Food Science and Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland. tracy.clegg@ucd.ie
authors.forename: Tracy A
authors.initials: TA
authors.lastname: Clegg
authors.forename: Anthony
authors.initials: A
authors.lastname: Duignan
authors.forename: Clare
authors.initials: C
authors.lastname: Whelan
authors.forename: Eamonn
authors.initials: E
authors.lastname: Gormley
authors.forename: Margaret
authors.initials: M
authors.lastname: Good
authors.forename: John
authors.initials: J
authors.lastname: Clarke
authors.forename: Nils
authors.initials: N
authors.lastname: Toft
authors.forename: Simon J
authors.initials: SJ
authors.lastname: More
mesh_terms: D000818:Animals; D001499:Bayes Theorem; D002417:Cattle / Q000276:immunology / Q000382:microbiology*; D007118:Immunoassay / Q000379:methods / Q000662:veterinary*; D007371:Interferon-gamma / Q000032:analysis*; D007428:Intradermal Tests / Q000379:methods / Q000662:veterinary; D007494:Ireland; D015233:Models, Statistical; D025202:Molecular Diagnostic Techniques / Q000379:methods / Q000662:veterinary; D012680:Sensitivity and Specificity; D014374:Tuberculin Test / Q000379:methods / Q000662:veterinary*; D014380:Tuberculosis, Bovine / Q000175:diagnosis* / Q000276:immunology
chemical_list: D007371:Interferon-gamma