title: Quantification of PrPC in bovine peripheral tissues: Analysis in wild-type and PrPC-deficient cattle.
abstract: Cellular PrP (PrPC) is necessary for bovine spongiform encephalopathy (BSE) infection. The purpose of the present experiment was the quantification of PrPC in peripheral tissues to assess the risk of BSE infection from these tissues. The tissue distribution of PrPC was examined by a sandwich enzyme-linked immunosorbent assay (sELISA) and histochemical analysis. PrPC-deficient cows were used as a negative control. The sELISA revealed that the brain contained the highest PrPC content (10.7 µg/g tissue), while other organs/tissues harbored lower amounts, in decreasing order as follows: longissimus capitis muscle, iliocostalis thoracis muscle, splenius muscle, biceps femoris muscle, triceps brachii muscle, longissimus thoracis muscle, ileum, jejunum, duodenum, colon, cecum, apex linguae, omotransversarius muscle, posterior part of the corpus linguae, anterior part of the corpus linguae and radix linguae (5.2- to 31-fold less PrPC than the brain). In the tissue/organs of PrP-deficient cows, PrPC levels were under the limit of detection. Histochemical analysis showed that PrPC was expressed in nerve cells in intestinal tissues. The presence of PrPC in the bovine tongue, skeletal muscles and intestines raises the possibility of PrPSc accumulation in these tissues, indicating that these organs/tissues may serve as potential sources of BSE infection.
authors.affiliation: School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan.
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Kobayashi
authors.forename: Yasuhisa
authors.initials: Y
authors.lastname: Ano
authors.forename: Akikazu
authors.initials: A
authors.lastname: Sakudo
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Yukawa
authors.forename: Katsuaki
authors.initials: K
authors.lastname: Sigiura
authors.forename: Noboru
authors.initials: N
authors.lastname: Manabe
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nakayama
authors.forename: Takashi
authors.initials: T
authors.lastname: Onodera