title: Electron tomography reveals polyhedrin binding and existence of both empty and full cytoplasmic polyhedrosis virus particles inside infectious polyhedra.
abstract: Previous studies have described the structure of purified cytoplasmic polyhedrosis virus (CPV) and that of polyhedrin protein. However, how polyhedrin molecules embed CPV particles inside infectious polyhedra is not known. By using electron tomography, we show that CPV particles are occluded within the polyhedrin crystalline lattice with a random spatial distribution and interact with the polyhedrin protein through the A-spike rather than as previously thought through the B-spike. Furthermore, both full (with RNA) and empty (no RNA) capsids were found inside polyhedra, suggesting a spontaneous RNA encapsidating process for CPV assembly in vivo.
authors.affiliation: Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA 90095, USA.
authors.forename: Justin
authors.initials: J
authors.lastname: Chen
authors.forename: Jingchen
authors.initials: J
authors.lastname: Sun
authors.forename: Ivo
authors.initials: I
authors.lastname: Atanasov
authors.forename: Sergey
authors.initials: S
authors.lastname: Ryazantsev
authors.forename: Z Hong
authors.initials: ZH
authors.lastname: Zhou
mesh_terms: D002213:Capsid / Q000378:metabolism; D055032:Electron Microscope Tomography / Q000379:methods; D007181:Inclusion Bodies, Viral / Q000648:ultrastructure / Q000821:virology*; D012087:Reoviridae / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D015678:Viral Structural Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014771:Virion / Q000302:isolation & purification / Q000378:metabolism*; D019065:Virus Assembly
chemical_list: D014764:Viral Proteins; D015678:Viral Structural Proteins