title: Endoribonuclease activities of porcine reproductive and respiratory syndrome virus nsp11 was essential for nsp11 to inhibit IFN-β induction.
abstract: Previous studies have shown that PRRSV nsp11, which was an endoribonuclease, was an interferon antagonist, however, the mechanism that nsp11 inhibited IFN-β production was unclear. To explore whether the endoribonuclease was required for nsp11 to disrupt the IFN-β production, substitutions of the presumed catalytic histidine and lysine residues of nsp11 were introduced into plasmid pcDNA 3.1-FLAG. The results showed that mutation that inactivated endoribonuclease made nsp11 lose its ability to inhibit Poly(I:C)-induced IFN-β promoter activity. In conclusion, our present work indicated that the endoribonuclease activity of nsp11 was essential for nsp11 to inhibit the IFN-β induction.
authors.affiliation: Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
authors.forename: Xibao
authors.initials: X
authors.lastname: Shi
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Xuewu
authors.initials: X
authors.lastname: Li
authors.forename: Gaiping
authors.initials: G
authors.lastname: Zhang
authors.forename: Junqing
authors.initials: J
authors.lastname: Guo
authors.forename: Dong
authors.initials: D
authors.lastname: Zhao
authors.forename: Shujun
authors.initials: S
authors.lastname: Chai
authors.forename: Ruiguang
authors.initials: R
authors.lastname: Deng
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D002522:Chlorocebus aethiops; D004722:Endoribonucleases / Q000378:metabolism*; D017930:Genes, Reporter; D006639:Histidine / Q000235:genetics; D016899:Interferon-beta / Q000096:biosynthesis* / Q000235:genetics; D008239:Lysine / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D011070:Poly I-C / Q000378:metabolism; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics / Q000378:metabolism*; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D017361:Viral Nonstructural Proteins; D006639:Histidine; D016899:Interferon-beta; D004722:Endoribonucleases; D008239:Lysine; D011070:Poly I-C