title: Hydroalcoholic extract based-ointment from Punica granatum L. peels with enhanced in vivo healing potential on dermal wounds.
abstract: The present study reports for the first time, the in vivo wound healing potential of Punica granatum L. peels. A 5% (w/w) methanolic extract based-ointment was formulated and evaluated for its wound healing in guinea pigs. The ointment was applied in vivo on the paravertebral area of twelve excised wounded models once a day for 10 consecutive days. The ointment significantly enhanced the wound contraction and the period of epithelialization as assessed by the mechanical (contraction rate, tensile strength), the biochemical (increasing of collagen, DNA and proteins synthesis) and the histopathological characteristics. Such investigation was encouraged by the efficiency of the methanolic extract as antimicrobial and antioxidant. Indeed, the extract showed antioxidant activity as strong as natural and synthetic compounds (Trolox, BHA, Quercetin). Furthermore, the extract exhibited significant antibacterial and antifungal activity against almost all tested bacteria: Pseudomonas aeruginosa ATCC 9027, Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Klebsiella pneumoniae, Salmonella anatum, Salmonella typhimurium, Streptococcus pneumoniae, and fungi Candida albicans, Candida glabrata, Trichopyton rubrum and Aspergillus niger. The formulated ointment might well find use as skin repair agent without hazard to human health based on these results and on the fact that it has been well established that the extracts of pomegranate used in conditions similar to those applied by traditional medicine, showed no toxic effects.
authors.affiliation: Laboratory of Bioactive Compounds at Biotechnology Center, Ecopark of Borj Cedria, BP-901, Hammam Lif 2050, Tunisia. a.hayouni@gmail.com
authors.forename: E A
authors.initials: EA
authors.lastname: Hayouni
authors.forename: K
authors.initials: K
authors.lastname: Miled
authors.forename: S
authors.initials: S
authors.lastname: Boubaker
authors.forename: Z
authors.initials: Z
authors.lastname: Bellasfar
authors.forename: M
authors.initials: M
authors.lastname: Abedrabba
authors.forename: H
authors.initials: H
authors.lastname: Iwaski
authors.forename: H
authors.initials: H
authors.lastname: Oku
authors.forename: T
authors.initials: T
authors.lastname: Matsui
authors.forename: F
authors.initials: F
authors.lastname: Limam
authors.forename: M
authors.initials: M
authors.lastname: Hamdi
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology; D000975:Antioxidants / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006168:Guinea Pigs; D029561:Lythraceae / Q000737:chemistry*; D008297:Male; D008826:Microbial Sensitivity Tests / Q000379:methods; D009824:Ointments / Q000737:chemistry / Q000494:pharmacology; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D012867:Skin / Q000187:drug effects* / Q000293:injuries; D013718:Tensile Strength; D014945:Wound Healing / Q000187:drug effects*; D014947:Wounds and Injuries / Q000188:drug therapy*
chemical_list: D000890:Anti-Infective Agents; D000975:Antioxidants; D009824:Ointments; D010936:Plant Extracts