title: Rescue of infectious particles from preassembled alphavirus nucleocapsid cores.
abstract: Alphaviruses are small, spherical, enveloped, positive-sense, single-stranded, RNA viruses responsible for considerable human and animal disease. Using microinjection of preassembled cores as a tool, a system has been established to study the assembly and budding process of Sindbis virus, the type member of the alphaviruses. We demonstrate the release of infectious virus-like particles from cells expressing Sindbis virus envelope glycoproteins following microinjection of Sindbis virus nucleocapsids purified from the cytoplasm of infected cells. Furthermore, it is shown that nucleocapsids assembled in vitro mimic those isolated in the cytoplasm of infected cells with respect to their ability to be incorporated into enveloped virions following microinjection. This system allows for the study of the alphavirus budding process independent of an authentic infection and provides a platform to study viral and host requirements for budding.
authors.affiliation: Markey Center for Structural Biology, Purdue University, West Lafayette, Indiana 47907, USA.
authors.forename: Jonathan E
authors.initials: JE
authors.lastname: Snyder
authors.forename: Odisse
authors.initials: O
authors.lastname: Azizgolshani
authors.forename: Bingbing
authors.initials: B
authors.lastname: Wu
authors.forename: Yingpei
authors.initials: Y
authors.lastname: He
authors.forename: Aih Cheun
authors.initials: AC
authors.lastname: Lee
authors.forename: Joyce
authors.initials: J
authors.lastname: Jose
authors.forename: Daniel M
authors.initials: DM
authors.lastname: Suter
authors.forename: Charles M
authors.initials: CM
authors.lastname: Knobler
authors.forename: William M
authors.initials: WM
authors.lastname: Gelbart
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Kuhn
mesh_terms: D000524:Alphavirus / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D006801:Humans; D007668:Kidney / Q000166:cytology / Q000821:virology*; D008845:Microinjections; D019251:Nucleocapsid / Q000008:administration & dosage* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012845:Sindbis Virus / Q000378:metabolism / Q000502:physiology; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism; D014771:Virion / Q000472:pathogenicity / Q000502:physiology*; D014773:Virology / Q000379:methods; D019065:Virus Assembly / Q000502:physiology*; D057074:Virus Release / Q000502:physiology*
chemical_list: D014759:Viral Envelope Proteins