title: A trypsin-like serine protease is involved in pseudorabies virus invasion through the basement membrane barrier of porcine nasal respiratory mucosa.
abstract: Several alphaherpesviruses breach the basement membrane during mucosal invasion. In the present study, the role of proteases in this process was examined. The serine protease-specific inhibitor AEBSF inhibited penetration of the basement membrane by the porcine alphaherpesvirus pseudorabies virus (PRV) by 88.1% without affecting lateral spread. Inhibitors of aspartic-, cysteine-, and metalloproteases did not inhibit viral penetration of the basement membrane. Further analysis using the Soybean Type I-S trypsin inhibitor for the serine protease subcategory of trypsin-like serine proteases resulted in a 96.9% reduction in plaque depth underneath the basement membrane. These data reveal a role of a trypsin-like serine protease in PRV penetration of the basement membrane.
authors.affiliation: Laboratory of Virology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium. Sarah.Glorieux@UGent.be.
authors.forename: Sarah
authors.initials: S
authors.lastname: Glorieux
authors.forename: Herman W
authors.initials: HW
authors.lastname: Favoreel
authors.forename: Lennert
authors.initials: L
authors.lastname: Steukers
authors.forename: Annelies P
authors.initials: AP
authors.lastname: Vandekerckhove
authors.forename: Hans J
authors.initials: HJ
authors.lastname: Nauwynck
mesh_terms: D000818:Animals; D001485:Basement Membrane / Q000648:ultrastructure / Q000821:virology*; D011558:Herpesvirus 1, Suid / Q000502:physiology*; D066298:In Vitro Techniques; D018613:Microscopy, Confocal / Q000662:veterinary; D009297:Nasal Mucosa / Q000648:ultrastructure / Q000821:virology*; D011480:Protease Inhibitors / Q000494:pharmacology; D011557:Pseudorabies / Q000821:virology*; D057057:Serine Proteases / Q000378:metabolism*; D015842:Serine Proteinase Inhibitors / Q000494:pharmacology; D013450:Sulfones / Q000494:pharmacology; D013552:Swine; D013553:Swine Diseases / Q000821:virology*; D010948:Viral Plaque Assay / Q000662:veterinary
chemical_list: D011480:Protease Inhibitors; D015842:Serine Proteinase Inhibitors; D013450:Sulfones; C002010:4-(2-aminoethyl)benzenesulfonylfluoride; D057057:Serine Proteases