title: Evaluation of chitosan gel containing liposome-loaded epidermal growth factor on burn wound healing.
abstract: The objective of this study is to develop a chitosan gel formulation containing liposomes loaded with epidermal growth factor (EGF) and to evaluate their effects on the healing of second-degree burn wounds in rats by immunohistochemical, histochemical and histological methods. EGF-containing multilamellar liposomes which were carried in chitosan gel, EGF gel and EGF-loaded liposome formulations were prepared. The in vivo experiments were performed on female Sprague Dawley rats. Second-degree standard burn wounds were formed on rats and liposomes containing 10 µg/ml EGF in 2% chitosan gel, EGF-chitosan gel and EGF-loaded liposome formulations were applied daily to the burn wounds and biopsies were taken at the 3rd, 7th and 14th day of the treatment. When the results were evaluated immunohistochemically, there were significant increases in cell proliferation observed in the EGF-containing liposome in chitosan gel (ELJ) formulation applied group (P < 0·001). The histochemical results showed that the epithelisation rate in the ELJ group was the highest compared with the other group results (P < 0·001). The histological results indicated and supported these findings and faster epithelisation was observed in the ELJ group compared with the other groups.
authors.affiliation: Department of Pharmaceutical Technology, Faculty of Pharmacy, Gazi University, Ankara, Turkey. zdegim@gazi.edu.tr
authors.forename: Zelihagül
authors.initials: Z
authors.lastname: Değim
authors.forename: Nevin
authors.initials: N
authors.lastname: Çelebi
authors.forename: Ceren
authors.initials: C
authors.lastname: Alemdaroğlu
authors.forename: Mustafa
authors.initials: M
authors.lastname: Deveci
authors.forename: Serdar
authors.initials: S
authors.lastname: Öztürk
authors.forename: Candan
authors.initials: C
authors.lastname: Özoğul
mesh_terms: D000287:Administration, Topical; D000818:Animals; D001672:Biocompatible Materials / Q000008:administration & dosage*; D001706:Biopsy; D002056:Burns / Q000188:drug therapy* / Q000473:pathology; D048271:Chitosan / Q000008:administration & dosage*; D004195:Disease Models, Animal; D004815:Epidermal Growth Factor / Q000008:administration & dosage*; D004817:Epidermis / Q000293:injuries / Q000473:pathology; D005260:Female; D005500:Follow-Up Studies; D005782:Gels; D006801:Humans; D008081:Liposomes; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000293:injuries* / Q000473:pathology; D016896:Treatment Outcome; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D001672:Biocompatible Materials; D005782:Gels; D008081:Liposomes; D004815:Epidermal Growth Factor; D048271:Chitosan