title: Radical scavenging and radiomodulatory effects of Psoralea corylifolia Linn. substantiated by in vitro assays and EPR spectroscopy.
abstract: The present study is the first report of the radiomodulatory effects of Psoralea corylifolia Linn. The extract (IBG-RA-26) prepared from P. corylifolia was chemically analysed by HPLC, LC-MS/MS and NMR. The total polyphenolic content of IBG-RA-26 was 0.287 mg/ml of quercetin equivalents. IBG-RA-26 exhibited a dose-dependent increase in 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity. It exhibited comparable (> 50%) site-specific and non-site-specific hydroxyl radical scavenging activity in higher concentration ranges (500-1000 microg/ml), while at lower concentrations (5-50 microg/ml) it exhibited significantly (p < 0.05) higher non-site-specific scavenging ability compared to site-specific activity. Nitric oxide scavenging activity of IBG-RA-26 (5-1000 microg/ml) increased in a concentration-dependent manner, while maximum superoxide ion scavenging ability (79%) was observed at 50 microg/ml. The electron donation potential of IBG-RA-26 was found to be higher than that of ascorbic acid at lower concentrations (up to 5 microg/ml). Analysis of the ability of IBG-RA-26 to protect membranes against gamma-radiation, utilizing an artificial membrane system (liposome), revealed a significant (p < 0.05) decrease in the formation of malondialdehyde (MDA) as a function of the concentration of IBG-RA-26. Radiation-induced lysis of human erythrocytes was monitored and efficacy of IBG-RA-26 was tested in the concentration range 25-1000 microg/ml, with significant protective efficacy observed in the range 25-50 microg/ml. IBG-RA-26 rendered significant (p < 0.05) protection against radiation (0.25 kGy)-induced DNA damage. EPR spectroscopy was used to investigate the DPPH radical scavenging capacity of IBG-RA-26. IBG-RA-26 exhibited a good DPPH radical scavenging capacity in a concentration-dependent manner. By direct EPR spectroscopy we have also demonstrated the possible formation of free radical species in a solution of IBG-RA-26. The wide spectrum of radioprotective and antioxidant properties exhibited by IBG-RA-26 indicate that P. corylifolia has potential as a radiomodulatory agent.
authors.affiliation: Department of Chemistry and Biochemistry, Medical Faculty, Trakia University, Armeiska St. 11, Stara Zagora 6000, Bulgaria. rajesharoradr@rediffmail.com
authors.forename: Rajesh
authors.initials: R
authors.lastname: Arora
authors.forename: Atlar Singh
authors.initials: AS
authors.lastname: Dhaker
authors.forename: Manish
authors.initials: M
authors.lastname: Adhikari
authors.forename: Jyoti
authors.initials: J
authors.lastname: Sharma
authors.forename: Raman
authors.initials: R
authors.lastname: Chawla
authors.forename: Damodar
authors.initials: D
authors.lastname: Gupta
authors.forename: Antoaneta
authors.initials: A
authors.lastname: Zheleva
authors.forename: Yanka
authors.initials: Y
authors.lastname: Karamalakova
authors.forename: Raj
authors.initials: R
authors.lastname: Kumar
authors.forename: Raj K
authors.initials: RK
authors.lastname: Sharma
authors.forename: Ashok
authors.initials: A
authors.lastname: Sharma
authors.forename: Sarwat
authors.initials: S
authors.lastname: Sultana
authors.forename: Rakesh K
authors.initials: RK
authors.lastname: Sharma
authors.forename: Rajendra P
authors.initials: RP
authors.lastname: Tripathi
authors.forename: Veselina
authors.initials: V
authors.lastname: Gadjeva
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology*; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy; D029907:Psoralea / Q000737:chemistry*; D011837:Radiation-Protective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D053719:Tandem Mass Spectrometry
chemical_list: D016166:Free Radical Scavengers; D011837:Radiation-Protective Agents