title: Identification and molecular cloning Moplaa gene, a homologue of Homo sapiens PLAA, in Magnaporthe oryzae.
abstract: Magnaporthe oryzae has been used as a model fungal pathogen to study the molecular basis of plant-fungus interactions due to its economic and genetic importance. In this study, we identified a novel gene, Moplaa, which is the homologue of Homo sapiens PLAA encoding a phospholipase A(2)-activating protein. Moplaa is conserved in some eukaryotic organisms by multiple alignment analysis. The function of the Moplaa gene was studied using the gene target replacement method. The Moplaa deletion mutant exhibited retarded growth and conidial germination, reduced conidiation, appressorial turgor pressure and pathogenicity to rice CO-39. Reintroduction of the gene restored defects of the Moplaa deletion mutant.
authors.affiliation: State Key Laboratory for Rice Biology, Biotechnology Institute, Zhejiang University, Hangzhou, China.
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Liu
authors.forename: Fei-Long
authors.initials: FL
authors.lastname: Zhuang
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Lu
authors.forename: Fu-Cheng
authors.initials: FC
authors.lastname: Lin
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D020082:Magnaporthe / Q000145:classification / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology; D011506:Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment; D013172:Spores, Fungal / Q000235:genetics / Q000254:growth & development / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D011506:Proteins; C055324:phospholipase A2-activating protein