title: The cyclin-dependent kinase inhibitor p27(Kip1) is a positive regulator of Schwann cell differentiation in vitro.
abstract: Schwann cell precursors differentiating into a myelinating phenotype are critical for peripheral nerve development and regeneration. However, little is known about the underlying molecular mechanisms of Schwann cell differentiation. In the present study, we performed a cyclic adenosine monophosphate-induced Schwann cell differentiation model in vitro. Western blot analysis showed that p27(Kip1) expression was upregulated during the differentiation of Schwann cell, while the inhibition of p27(Kip1) expression by short hairpin RNA-mediated knockdown significantly abolished the expression of promyelinating markers and the alteration of cellular morphology. In addition, immunofluorescence revealed a decrease of p27(Kip1) nuclear staining and a concomitant increase of cytoplasmic staining in differentiated Schwann cells. In summary, our data indicated that p27(Kip1) was a positive regulator of Schwann cell differentiation in vitro.
authors.affiliation: Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong 22600, People's Republic of China.
authors.forename: Honghui
authors.initials: H
authors.lastname: Li
authors.forename: Huiguang
authors.initials: H
authors.lastname: Yang
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Liu
authors.forename: Weipeng
authors.initials: W
authors.lastname: Huan
authors.forename: Shuangwei
authors.initials: S
authors.lastname: Zhang
authors.forename: Gang
authors.initials: G
authors.lastname: Wu
authors.forename: Qiuhui
authors.initials: Q
authors.lastname: Lu
authors.forename: Qiuhong
authors.initials: Q
authors.lastname: Wang
authors.forename: Youhua
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002453:Cell Cycle / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000494:pharmacology; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D044786:S-Phase Kinase-Associated Proteins / Q000378:metabolism; D012583:Schwann Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D043743:Ubiquitin-Protein Ligase Complexes; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism
chemical_list: D015415:Biomarkers; D034741:RNA, Small Interfering; D044786:S-Phase Kinase-Associated Proteins; C558223:Ubac1 protein, rat; D050760:Cyclin-Dependent Kinase Inhibitor p27; D000242:Cyclic AMP; D043743:Ubiquitin-Protein Ligase Complexes; D044767:Ubiquitin-Protein Ligases