title: Soluble vascular endothelial growth factor (VEGF) receptor-1 inhibits migration of human monocytic THP-1 cells in response to VEGF.
abstract: OBJECTIVE
  We aimed to investigate the regulation and contribution of vascular endothelial growth factor (VEGF) and sFlt-1(1-3) to human monocytic THP-1 migration.
  
  
  METHODS
  Ad-sFlt-1/FLAG, a recombinant adenovirus carrying the human sFlt-1(1-3) (the first three extracellular domains of FLT-1, the hVEGF receptor-1) gene, was constructed. L929 cells were infected with Ad-sFlt-1/FLAG and the expression of sFlt-1 was detected by immunofluorescent assay and ELISA. Corning(®) Transwell(®) Filter Inserts containing polyethylene terephthalate (PET) membranes with pore sizes of 3 μm were used as an experimental model to simulate THP-1 migration. Five VEGF concentrations (0, 0.1, 1, 10 and 100 ng/ml), four concentrations of sFlt-1(1-3)/FLAG expression supernatants (0.1, 1, 10 and 100 ng/ml), and monocyte chemoattractant protein-1 (MCP-1, 10 ng/ml) were used to test the ability of THP-1 cells to migrate through PET membranes.
  
  
  RESULTS
  The sFlt-1(1-3) gene was successfully recombined into Ad-sFlt-1/FLAG. sFlt-1(1-3) was expressed in L929 cells transfected with Ad-sFlt-1/FLAG. THP-1 cell migration increased with increasing concentrations of VEGF, while cell migration decreased with increasing concentrations of sFlt1(1-3)/FLAG. sFlt1(1-3)/FLAG had no effect on MCP-1-induced cell migration.
  
  
  CONCLUSIONS
  This study demonstrated that VEGF is able to elicit a migratory response in THP-1 cells, and that sFlt-1(1-3) is an effective inhibitor of THP-1 migration towards VEGF.
authors.affiliation: Department of Neurology, The Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou, People's Republic of China. canshengzhu@yahoo.com.cn
authors.forename: Cansheng
authors.initials: C
authors.lastname: Zhu
authors.forename: Zhaojun
authors.initials: Z
authors.lastname: Xiong
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Chen
authors.forename: Zhengqi
authors.initials: Z
authors.lastname: Lu
authors.forename: Guoyu
authors.initials: G
authors.lastname: Zhou
authors.forename: Dunjing
authors.initials: D
authors.lastname: Wang
authors.forename: Jian
authors.initials: J
authors.lastname: Bao
authors.forename: Xueqiang
authors.initials: X
authors.lastname: Hu
mesh_terms: D000818:Animals; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects*; D006801:Humans; D051379:Mice; D009000:Monocytes / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism* / Q000494:pharmacology*; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000235:genetics / Q000378:metabolism*; D055815:Young Adult
chemical_list: D011993:Recombinant Fusion Proteins; D042461:Vascular Endothelial Growth Factor A; D040281:Vascular Endothelial Growth Factor Receptor-1