title: [Deficiency of water channel AQP4 aggravates NMDA-induced brain injury in mice].
abstract: To evaluate the role of water channel AQP4 in NMDA-induced brain injury in mice.
  
  
  
  In AQP4 gene knockout (AQP4(-/-)) mice, brain injury was induced by microinjection of NMDA into the cortex. The injured area was determined by toluidine blue staining, degenerated neurons were detected by Fluro-Jade B staining, and increased blood-brain barrier (BBB) permeability was evaluated by IgG immunostaining.
  
  
  
  Compared with wild-type mice, AQP4(-/-) mice exhibited increased cortical lesion area, aggravated neuron degeneration, and increased BBB disruption after NMDA microinjection.
  
  
  
  AQP4 may play a protective role in NMDA-induced brain injury in mice.
authors.affiliation: Department of Pharmacology, College of Medicine, Zhejiang University, Hangzhou 310058, China.
authors.forename: Wen-Zhen
authors.initials: WZ
authors.lastname: Shi
authors.forename: Chun-Zhen
authors.initials: CZ
authors.lastname: Zhao
authors.forename: Xue-Qin
authors.initials: XQ
authors.lastname: Huang
authors.forename: Wei-Ping
authors.initials: WP
authors.lastname: Zhang
authors.forename: San-Hua
authors.initials: SH
authors.lastname: Fang
authors.forename: Yun-Bi
authors.initials: YB
authors.lastname: Lu
authors.forename: Er-Qing
authors.initials: EQ
authors.lastname: Wei
mesh_terms: D000818:Animals; D051401:Aquaporin 4 / Q000235:genetics* / Q000502:physiology; D001812:Blood-Brain Barrier / Q000473:pathology; D001921:Brain / Q000187:drug effects / Q000473:pathology*; D051379:Mice; D018345:Mice, Knockout; D016202:N-Methylaspartate / Q000633:toxicity*
chemical_list: C496031:Aqp4 protein, mouse; D051401:Aquaporin 4; D016202:N-Methylaspartate