title: [Fenotypic and genotypic characterization of Shiga toxin-producing Escherichia coli O111 strain isolated from patient with hemolytic-uremic syndrome].
abstract: Shiga toxin-producing E. coli O157 and non-O157 are important emergance pathogens that can cause diarrhea and hemorrhagic colitis with life-threatening complications, such as hemolytic uremic syndrome (HUS). A few cases of EHEC infections are documented per year in Poland. Among them only one patient with EHEC O157 infection developed HUS. We characterized the first VTEC non-O157 strain isolated from child with HUS in Poland. The VTEC O111 strain produced Stx2 which was cytotoxic for Vero cell. Using DNA microarray analysis we have found set of virulence genes in VTEC O111 strain as: stx2A, stx2B, ehly, eae, tir tccP espA, espJ, cif nleA, nleB, lpfA, iha, efa1, cba. The strain was fenotypic resistant to streptomycin, tetracyclin and sulphonamides (strA, tetA, sul2 genes were detected).
authors.affiliation: Zakład Bakteriologii Narodowego Instytutu Zdrowia Publicznego-Paistwowego Zakladu Higieny w Warszawie.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Januszkiewicz
authors.forename: Edyta
authors.initials: E
authors.lastname: Podsiadły
authors.forename: Jolanta
authors.initials: J
authors.lastname: Szych
authors.forename: Anna
authors.initials: A
authors.lastname: Semkowicz-Chmielewska
authors.forename: Urszula
authors.initials: U
authors.lastname: Demkow
authors.forename: Anna
authors.initials: A
authors.lastname: Pierzchlewicz
authors.forename: Waldemar
authors.initials: W
authors.lastname: Rastawicki
mesh_terms: D004926:Escherichia coli / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D005838:Genotype; D006463:Hemolytic-Uremic Syndrome / Q000382:microbiology*; D006801:Humans; D010641:Phenotype; D022621:Shiga Toxin / Q000096:biosynthesis*; D013045:Species Specificity; D014774:Virulence / Q000235:genetics*
chemical_list: D022621:Shiga Toxin