title: B6-MSM consomic mouse strains reveal multiple loci for genetic variation in sucrose octaacetate aversion.
abstract: Based on crosses among inbred strains derived principally from M. m. domesticus, sucrose octaacetate (SOA) aversion in laboratory mice has been thought for many years to be controlled by a single genetic locus (Soa) located on distal chromosome (Chr) 6. To expand knowledge of the genetic basis underlying SOA aversion, we have studied the M. m. molossinus derived strain (MSM) and MSM consomic strains on a M. m. domesticus (C57BL/6J: B6) host background. Using two-bottle preference procedures, MSM mice avoided 0.1 mM and 1 mM SOA while B6 mice were indifferent to 0.1 mM and exhibited slight aversion to 1 mM SOA. Preference tests of 16 available consomic strains implicated Chr 2, 4 and 15 in SOA aversion in addition to the prominent effect of the known Soa locus on Chr 6 (implicated by study of two congenic strains). The originally defined Soa locus is presumably associated with one or more members of the cluster of Tas2r genes on distal Chr 6 that code for bitter taste receptors. Our results point to the possible role of established Tas2r genes on Chr 2 and 15, as well as to genes not coding for bitter receptors (Chr 4), in SOA aversion. SOA aversion emerges from this consomic screen as being definitively under polygenic control. The genetic diversity captured by the MSM model system is shown to be a valuable tool to complement the limited genetic variation in the commonly used stocks derived from M m. domesticus.
authors.affiliation: Mouse Genomics Resource Laboratory, National Institute of Genetics, Mishima, Japan.
authors.forename: Ayako
authors.initials: A
authors.lastname: Ishii
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Koide
authors.forename: Aki
authors.initials: A
authors.lastname: Takahashi
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Shiroishi
authors.forename: Thomas P
authors.initials: TP
authors.lastname: Hettinger
authors.forename: Marion E
authors.initials: ME
authors.lastname: Frank
authors.forename: Lawrence D
authors.initials: LD
authors.lastname: Savoy
authors.forename: Bradley K
authors.initials: BK
authors.lastname: Formaker
authors.forename: Sezen
authors.initials: S
authors.lastname: Yertutanol
authors.forename: Arimantas
authors.initials: A
authors.lastname: Lionikas
authors.forename: David A
authors.initials: DA
authors.lastname: Blizard
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D002755:Choice Behavior; D003433:Crosses, Genetic; D005260:Female; D014644:Genetic Variation; D008297:Male; D051379:Mice; D020297:Mice, Congenic; D008810:Mice, Inbred C57BL; D008957:Models, Genetic; D013395:Sucrose / Q000031:analogs & derivatives* / Q000494:pharmacology; D013649:Taste; D013652:Taste Threshold / Q000502:physiology
chemical_list: C026879:sucrose octaacetate; D013395:Sucrose