title: [5-lipoxygenase is involved in rotenone-induced injury in PC12 cells].
abstract: To determine whether 5-lipoxygenase (5-LOX) is involved in rotenone-induced injury in PC12 cells, which is a cell model of Parkinson disease.
  
  
  
  After rotenone treatment for various durations, cell viability was determined by colorimetric MTT reduction assay, and 5-LOX translocation was detected by immunocytochemistry. The effect of 5-LOX inhibitor zileuton was also investigated.
  
  
  
  Rotenone (0.3-30 μmol/L) induced PC12 cell injury, and zileuton (3-100 μmol/L) attenuated this injury. Rotenone also time-and concentration-dependently induced 5-LOX translocation into the nuclear envelope, and zileuton (1-30 μmo/L) significantly inhibited rotenone-induced 5-LOX translocation.
  
  
  
  5-LOX is involved in rotenone-induced injury in PC12 cells, and 5-LOX inhibitor zileuton can reduce rotenone-induced 5-LOX activation and cell injury.
authors.affiliation: Hangzhou Key Laboratory of Neurobiology and Department of Pharmacology, School of Basic Medicine, Hangzhou Normal University, Hangzhou 310036, China.
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Zhang
authors.forename: Li-Hui
authors.initials: LH
authors.lastname: Zhang
authors.forename: Cheng-Tan
authors.initials: CT
authors.lastname: Li
authors.forename: Wen-Jian
authors.initials: WJ
authors.lastname: Chen
authors.forename: Jian-Bo
authors.initials: JB
authors.lastname: Zhao
authors.forename: Er-Qing
authors.initials: EQ
authors.lastname: Wei
mesh_terms: D000818:Animals; D001094:Arachidonate 5-Lipoxygenase / Q000378:metabolism / Q000502:physiology*; D002470:Cell Survival / Q000187:drug effects; D006918:Hydroxyurea / Q000031:analogs & derivatives / Q000494:pharmacology; D016859:Lipoxygenase Inhibitors / Q000494:pharmacology; D016716:PC12 Cells; D051381:Rats; D012402:Rotenone / Q000494:pharmacology*
chemical_list: D016859:Lipoxygenase Inhibitors; D012402:Rotenone; D001094:Arachidonate 5-Lipoxygenase; C063449:zileuton; D006918:Hydroxyurea