title: Effects of molybdate and tungstate on expression levels and biochemical characteristics of formate dehydrogenases produced by Desulfovibrio alaskensis NCIMB 13491.
abstract: Formate dehydrogenases (FDHs) are enzymes that catalyze the formate oxidation to carbon dioxide and that contain either Mo or W in a mononuclear form in the active site. In the present work, the influence of Mo and W salts on the production of FDH by Desulfovibrio alaskensis NCIMB 13491 was studied. Two different FDHs, one containing W (W-FDH) and a second incorporating either Mo or W (Mo/W-FDH), were purified. Both enzymes were isolated from cells grown in a medium supplemented with 1 μM molybdate, whereas only the W-FDH was purified from cells cultured in medium supplemented with 10 μM tungstate. We demonstrated that the genes encoding the Mo/W-FDH are strongly downregulated by W and slightly upregulated by Mo. Metal effects on the expression level of the genes encoding the W-FDH were less significant. Furthermore, the expression levels of the genes encoding proteins involved in molybdate and tungstate transport are downregulated under the experimental conditions evaluated in this work. The molecular and biochemical properties of these enzymes and the selective incorporation of either Mo or W are discussed.
authors.affiliation: REQUIMTE/CQFB, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
authors.forename: Cristiano S
authors.initials: CS
authors.lastname: Mota
authors.forename: Odile
authors.initials: O
authors.lastname: Valette
authors.forename: Pablo J
authors.initials: PJ
authors.lastname: González
authors.forename: Carlos D
authors.initials: CD
authors.lastname: Brondino
authors.forename: José J G
authors.initials: JJ
authors.lastname: Moura
authors.forename: Isabel
authors.initials: I
authors.lastname: Moura
authors.forename: Alain
authors.initials: A
authors.lastname: Dolla
authors.forename: Maria G
authors.initials: MG
authors.lastname: Rivas
mesh_terms: D003901:Desulfovibrio / Q000201:enzymology* / Q000378:metabolism; D005560:Formate Dehydrogenases / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D008982:Molybdenum / Q000494:pharmacology*; D014414:Tungsten / Q000494:pharmacology*
chemical_list: D008982:Molybdenum; D005560:Formate Dehydrogenases; D014414:Tungsten