title: Molecular detection of Torque teno sus virus in lymphoid tissues in concomitant infections with other porcine viral pathogens.
abstract: In this study, 40 pigs with respiratory and wasting disorders from Cuban swine herds were screened by PCR for the presence of TTSuV1, TTSuV2, PCV-2, PPV and CSFV in spleen samples. The variability of the porcine TTSuV sequences obtained was investigated by phylogenetic analysis. This study showed for the first time that TTSuV1 and TTSuV2 were present in Cuban swine herds. The investigation revealed the following infection rates: TTSuV1 40%, TTSuV2 37.5%, PCV-2 70%, PPV 37.5% and CSFV in 52.5%. The presence of two or more of these viruses at different rates in the same spleen samples was revealed. Also, a higher genetic diversity of TTSuV2 sequences was observed regarding TTSuV1 sequences.
authors.affiliation: Centro Nacional de Sanidad Agropecuaria (CENSA), La Habana, Cuba.
authors.forename: Lester J
authors.initials: LJ
authors.lastname: Pérez
authors.forename: Heidy Díaz
authors.initials: HD
authors.lastname: de Arce
authors.forename: María T
authors.initials: MT
authors.lastname: Frias
authors.forename: Carmen L
authors.initials: CL
authors.lastname: Perera
authors.forename: Llilianne
authors.initials: L
authors.lastname: Ganges
authors.forename: José I
authors.initials: JI
authors.lastname: Núñez
mesh_terms: D000818:Animals; D001483:Base Sequence; D017925:Circovirus / Q000302:isolation & purification; D006692:Classical Swine Fever Virus / Q000302:isolation & purification; D004266:DNA Virus Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D004279:DNA, Viral / Q000235:genetics; D028323:Parvovirus, Porcine / Q000302:isolation & purification; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D013154:Spleen / Q000821:virology*; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis / Q000821:virology*; D022783:Torque teno virus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D004279:DNA, Viral