title: Metabolic stress promotes renal tubular inflammation by triggering the unfolded protein response.
abstract: The renal epithelium contributes to the development of inflammation during ischemic injury. Ischemia induces endoplasmic reticulum (ER) stress and activates the unfolded protein response (UPR). Ischemic tissues generate distress signals and inflammation that activates fibrogenesis and may promote adaptive immunity. Interestingly, the UPR may activate inflammation pathways. Our aim was to test whether the UPR is activated during metabolic stress and mediates a tubular inflammatory response. Glucose deprivation, not hypoxia and amino acids deprivation, activated the UPR in human renal cortical tubular cells in culture. This stress activated NF-κB and promoted the transcription of proinflammatory cytokines and chemokines, including IL-6, IL-8, TNF-α, RANTES and MCP-1. The protein kinase RNA (PKR)-like ER kinase signaling pathway was not required for the induction of inflammation but amplified cytokine. Inositol-requiring enzyme 1 activated NF-κB signaling and was required for the transcription of proinflammatory cytokines and chemokines following metabolic stress. Moreover, acute ischemia activated ER stress and inflammation in rat kidneys. Finally, the ER stress marker GRP78 and NF-κB p65/RelA were coexpressed in human kidney transplants biopsies performed before implantation, suggesting that ER stress activates tubular inflammation in human renal allografts. In conclusion, this study establishes a link between ischemic stress, the activation of the UPR and the generation of a tubular inflammatory response.
authors.affiliation: INSERM U, Université Paris Descartes, France.
authors.forename: S
authors.initials: S
authors.lastname: Fougeray
authors.forename: N
authors.initials: N
authors.lastname: Bouvier
authors.forename: P
authors.initials: P
authors.lastname: Beaune
authors.forename: C
authors.initials: C
authors.lastname: Legendre
authors.forename: D
authors.initials: D
authors.lastname: Anglicheau
authors.forename: E
authors.initials: E
authors.lastname: Thervet
authors.forename: N
authors.initials: N
authors.lastname: Pallet
mesh_terms: D000818:Animals; D018925:Chemokines / Q000276:immunology; D016207:Cytokines / Q000276:immunology; D004721:Endoplasmic Reticulum / Q000276:immunology; D000091342:Endoplasmic Reticulum Chaperone BiP; D006801:Humans; D007511:Ischemia / Q000150:complications; D007684:Kidney Tubules / Q000098:blood supply / Q000276:immunology* / Q000502:physiology; D008297:Male; D008565:Membrane Proteins / Q000276:immunology; D016328:NF-kappa B / Q000235:genetics / Q000276:immunology; D017346:Protein Serine-Threonine Kinases / Q000276:immunology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013312:Stress, Physiological; D056811:Unfolded Protein Response
chemical_list: D018925:Chemokines; D016207:Cytokines; D000091342:Endoplasmic Reticulum Chaperone BiP; C000717247:HSPA5 protein, human; D008565:Membrane Proteins; D016328:NF-kappa B; C486557:Ern2 protein, rat; D017346:Protein Serine-Threonine Kinases