title: [Comparative study of somatic oncogene mutations in normal thyroid tissues and thyroid neoplasms].
abstract: It is established that numerous somatic oncogene mutation (BRAF, NRAS, HRAS, KRAS) and gene translocations (RET/PTC, PAX8/PPAR-gamma) are associated with the development of thyroid cancer. In this study 22 intraoperative thyroid tissue samples (11 pathologic and 11 normal) were examined. Somatic single nucleotide polymorphisms were analyzed by LigthCycler melting method, while translocations were identified by real-time polymerase chain reaction technique. In tumorous sample 3 BRAF, 2 NRAS and one HRAS mutations were found, as well as one RET/PTC1 translocation. Results confirm international data showing that these oncogene mutations and translocations are linked to thyroid cancer. Cytological examination completed with genetic data may support the diagnosis of thyroid malignancies. In addition, genetic alterations may indicate malignant transformation and may become prognostic factors in future.
authors.affiliation: Semmelweis Egyetem, Általános Orvostudományi Kar I. Belgyógyászati Klinika Budapest Korányi S. u. 2/A 1083. tobiasb@bell.sote.hu
authors.forename: Bálint
authors.initials: B
authors.lastname: Tóbiás
authors.forename: Bernadett
authors.initials: B
authors.lastname: Balla
authors.forename: János
authors.initials: J
authors.lastname: Kósa P
authors.forename: János
authors.initials: J
authors.lastname: Horányi
authors.forename: István
authors.initials: I
authors.lastname: Takács
authors.forename: Eszter
authors.initials: E
authors.lastname: Bölöny
authors.forename: Csaba
authors.initials: C
authors.lastname: Halászlaki
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Nagy
authors.forename: Gábor
authors.initials: G
authors.lastname: Speer
authors.forename: Balázs
authors.initials: B
authors.lastname: Járay
authors.forename: Eszter
authors.initials: E
authors.lastname: Székely
authors.forename: Roland
authors.initials: R
authors.lastname: Istók
authors.forename: Péter
authors.initials: P
authors.lastname: Lakatos
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D011905:Genes, ras / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D015513:Oncogene Proteins / Q000235:genetics*; D000072079:Patched Receptors; D020641:Polymorphism, Single Nucleotide; D048493:Proto-Oncogene Proteins B-raf / Q000235:genetics; D051096:Proto-Oncogene Proteins c-ret / Q000235:genetics; D011956:Receptors, Cell Surface / Q000235:genetics; D013961:Thyroid Gland / Q000378:metabolism*; D013964:Thyroid Neoplasms / Q000235:genetics*
chemical_list: D015513:Oncogene Proteins; D000072079:Patched Receptors; D011956:Receptors, Cell Surface; D051096:Proto-Oncogene Proteins c-ret; C099282:RET protein, human; C482119:BRAF protein, human; D048493:Proto-Oncogene Proteins B-raf