title: Species diversity of Trichoderma in Poland.
abstract: In the present study, we reinvestigate the diversity of Trichoderma in Poland utilizing a combination of morphological and molecular/phylogenetic methods. A total of 170 isolates were collected from six different substrata at 49 sites in Poland. These were divided among 14 taxa as follows: 110 of 170 Trichoderma isolates were identified to the species level by the analysis of their ITS1, ITS2 rDNA sequences as: T. harzianum (43 isolates), T. aggressivum (35), T. citrinoviride (11), T. hamatum (9), T. virens (6), T. longibrachiatum (4), T. polysporum (1), and T. tomentosum (1); 60 isolates belonging to the Viride clade were identified based on a fragment of the translation-elongation factor 1-alpha (tef1) gene as: T. atroviride (20 isolates), T. gamsii (2), T. koningii (17), T. viridescens (13), T. viride (7), and T. koningiopsis (1). Identifications were made using the BLAST interface in TrichOKEY and TrichoBLAST ( http://www.isth.info ). The most diverse substrata were soil (nine species per 22 isolates) and decaying wood (nine species per 75 isolates). The most abundant species (25%) isolated from all substrata was T. harzianum.
authors.affiliation: Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479, Poznań, Poland. lgol@igr.poznan.pl
authors.forename: Lidia
authors.initials: L
authors.lastname: Błaszczyk
authors.forename: Delfina
authors.initials: D
authors.lastname: Popiel
authors.forename: Jerzy
authors.initials: J
authors.lastname: Chełkowski
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Koczyk
authors.forename: Gary J
authors.initials: GJ
authors.lastname: Samuels
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Sobieralski
authors.forename: Marek
authors.initials: M
authors.lastname: Siwulski
mesh_terms: D004275:DNA, Ribosomal / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D014644:Genetic Variation; D008969:Molecular Sequence Data; D025282:Mycelium / Q000235:genetics; D010444:Peptide Elongation Factor Tu / Q000235:genetics; D010802:Phylogeny; D058974:Phylogeography; D011044:Poland; D017422:Sequence Analysis, DNA; D014242:Trichoderma / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D010444:Peptide Elongation Factor Tu