title: [Identification and expression analysis of Macaca mulatta piwil4 gene].
abstract: To investigate the structure and expression pattern of rhesus monkey PIWIL4 protein, homologous comparison and reverse transcription PCR (RT-PCR) were carried out to identify rhesus monkey piwil4. The expression of piwil4 mRNA was tested in rhesus monkey heart, brain, colon, epididymis and testis, and the result showed that piwil4 mRNA was expressed in these rhesus monkey tissues. Bioinformatic analysis suggested that the rhesus PIWIL4 protein shared 97% identity in amino acids and the same domains such as PAZ and Piwi with the human PIWIL4 (HIWI2) protein. The immunohistochemical result indicated that PIWIL4 proteins had the same localization in adult testes of the two species, but the distribution of these proteins was altered dynamically at different developmental stages in rhesus monkey testes. PIWIL4 protein was expressed in the nucleus of convoluted seminiferous tubules in infant monkey testes, whereas it was expressed in the cytoplasm of adult monkey testes. The results suggest that piwil4 gene play a similar role in rhesus and human, and different localizations of PIWIL4 protein in infant monkey and adult monkey testes suggest that it functions differently at different developmental stages.
authors.affiliation: Department of Medical Genetics and Division of Human Morbid Genomics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China. teszsz@163.com
authors.forename: Shao-Zhi
authors.initials: SZ
authors.lastname: Zhao
authors.forename: Yue
authors.initials: Y
authors.lastname: Li
authors.forename: Xue
authors.initials: X
authors.lastname: Jiang
authors.forename: Yi-Lu
authors.initials: YL
authors.lastname: Lu
authors.forename: Da-Chang
authors.initials: DC
authors.lastname: Tao
authors.forename: Yun-Qiang
authors.initials: YQ
authors.lastname: Liu
authors.forename: Yong-Xin
authors.initials: YX
authors.lastname: Ma
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D060565:Argonaute Proteins; D006801:Humans; D007150:Immunohistochemistry; D008253:Macaca mulatta / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D011506:Proteins / Q000032:analysis / Q000235:genetics*; D016601:RNA-Binding Proteins; D013737:Testis / Q000737:chemistry
chemical_list: D060565:Argonaute Proteins; C556795:PIWIL4 protein, human; D011506:Proteins; D016601:RNA-Binding Proteins