title: Cell response to collagen-calcium phosphate cement scaffolds investigated for nonviral gene delivery.
abstract: Collagen-hydroxyapatite (HA) scaffolds for the non-viral delivery of a plasmid encoding the osteoinductive protein bone morphogenetic protein (BMP)-7 were developed. The collagen-HA was obtained by the combination of calcium phosphate cement in a collagen template. The effect on cell behavior of increasing amounts of HA in the scaffolds was evaluated. Collagen-HA scaffolds containing 13, 23 or 83 wt% HA were prepared. Cell proliferation was reduced in the 83% HA scaffold after 1 day compared to 13 and 23% HA, but by 14 days the number of cells in 83% HA considerably increased. Alkaline phosphatase (ALP) activity was 8 times higher for the 83% HA scaffolds. BMP-7 plasmid was incorporated into the 83% HA scaffold. The transfection was low, although significant levels of BMP7 were expressed, associated with an increase in cell proliferation.
authors.affiliation: Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Metallurgy, Technical University of Catalonia, UPC, 647 Diagonal Av, 08028 Barcelona, Spain.
authors.forename: R A
authors.initials: RA
authors.lastname: Perez
authors.forename: M P
authors.initials: MP
authors.lastname: Ginebra
authors.forename: M
authors.initials: M
authors.lastname: Spector
mesh_terms: D000469:Alkaline Phosphatase / Q000737:chemistry; D000818:Animals; D001843:Bone Cements / Q000737:chemistry*; D055419:Bone Morphogenetic Protein 7 / Q000378:metabolism; D002130:Calcium Phosphates / Q000737:chemistry*; D049109:Cell Proliferation; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry*; D018014:Gene Transfer Techniques; D006041:Goats; D059630:Mesenchymal Stem Cells / Q000166:cytology; D008956:Models, Chemical; D010957:Plasmids / Q000378:metabolism; D013552:Swine; D014961:X-Ray Diffraction
chemical_list: D001843:Bone Cements; D055419:Bone Morphogenetic Protein 7; D002130:Calcium Phosphates; D003094:Collagen; C020243:calcium phosphate; D000469:Alkaline Phosphatase