title: Virus-induced differential expression of nuclear receptors and coregulators in dendritic cells: implication to interferon production.
abstract: We investigated mRNA expression of 49 nuclear hormone receptors (NRs) and 35 transcriptional coregulators in mouse bone marrow-derived dendritic cells (DCs) upon infection with Newcastle Disease virus (NDV) or murine cytomegalovirus (MCMV). These viruses regulated mRNA expression of some NRs among which NOR1 and LXRα were highly induced at mRNA and protein levels. Exogenous expression of the latter NRs repressed IRF3- or IRF7-induced transactivation of the interferon β promoter and NDV infection further potentiated their repressive effect. The viral infection also significantly regulated mRNA expression of some coregulators, including HDAC1. Toll-like receptor ligands regulated NR and coregulator mRNA expression similar to the viruses. Thus, NRs and coregulators are integral components of DC-organizing anti-viral response wherein NOR1 and LXRα participate in regulating interferon production.
authors.affiliation: Unit on Molecular Hormone Action, Program in Reproductive and Adult Endocrinology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892-1109, USA.
authors.forename: Sinnie Sin Man
authors.initials: SS
authors.lastname: Ng
authors.forename: Tsung-Hsien
authors.initials: TH
authors.lastname: Chang
authors.forename: Prafullakumar
authors.initials: P
authors.lastname: Tailor
authors.forename: Keiko
authors.initials: K
authors.lastname: Ozato
authors.forename: Tomoshige
authors.initials: T
authors.lastname: Kino
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D003713:Dendritic Cells / Q000378:metabolism* / Q000821:virology*; D020869:Gene Expression Profiling; D045325:HCT116 Cells; D056284:Histone Deacetylase 1 / Q000235:genetics / Q000378:metabolism*; D054884:Host-Pathogen Interactions; D006801:Humans; D015151:Immunoblotting; D016898:Interferon-alpha / Q000235:genetics / Q000378:metabolism; D000071518:Liver X Receptors; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018146:Muromegalovirus / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D009522:Newcastle disease virus / Q000502:physiology*; D057093:Orphan Nuclear Receptors / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics / Q000378:metabolism*; D011987:Receptors, Steroid / Q000235:genetics / Q000378:metabolism; D011988:Receptors, Thyroid Hormone / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D004268:DNA-Binding Proteins; D016898:Interferon-alpha; D000071518:Liver X Receptors; C000605213:NR1H3 protein, human; D009419:Nerve Tissue Proteins; C000605214:Nr1h3 protein, mouse; C490411:Nr4a3 protein, mouse; D057093:Orphan Nuclear Receptors; D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear; D011987:Receptors, Steroid; D011988:Receptors, Thyroid Hormone; C484389:Hdac1 protein, mouse; D056284:Histone Deacetylase 1