title: Performance and attributes of liquid chromatography-mass spectrometry with targeted charge separation in quantitative analysis of therapeutic peptides.
abstract: Herein we describe a new method, targeted enhanced multiply charged scans (tEMC), for the quantification of therapeutic peptides in tandem mass spectrometry on the linear ion trap mass spectrometer. Therapeutic peptides with chain lengths between eight and 39 amino acid residues and charge states from 2+ to 6+ were used to evaluate and illustrate the method which relies on the ability to separate ions trapped in a linear ion trap according to their charges. In particular, interference from singly charged ions on multiply charged ions can be effectively minimized. The method requires optimization of relatively few parameters, the most important of which being the exit lens barrier (EXB) voltage, thereby offering substantial time saving in a high-throughput quantification environment that currently relies on selected reaction monitoring.
authors.affiliation: Department of Chemistry and Centre for Research in Mass Spectrometry, York University, 4700 Keele Street, Toronto, ON M3J 1P3, Canada.
authors.forename: Changtong
authors.initials: C
authors.lastname: Hao
authors.forename: J Larry
authors.initials: JL
authors.lastname: Campbell
authors.forename: Udo H
authors.initials: UH
authors.lastname: Verkerk
authors.forename: J C Yves
authors.initials: JC
authors.lastname: Le Blanc
authors.forename: K W Michael
authors.initials: KW
authors.lastname: Siu
mesh_terms: D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000032:analysis*; D001516:Bees; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D009479:Neuropeptides / Q000032:analysis* / Q000097:blood; D036361:Peptide Hormones / Q000032:analysis* / Q000097:blood; D015203:Reproducibility of Results; D013552:Swine
chemical_list: D023181:Antimicrobial Cationic Peptides; D009479:Neuropeptides; D036361:Peptide Hormones