title: Interaction trap/two-hybrid system to identify interacting proteins.
abstract: The yeast two-hybrid method (or interaction trap) is a powerful technique for detecting protein interactions. The procedure is performed using transcriptional activation of a dual reporter system in yeast to identify interactions between a protein of interest (the bait protein) and the candidate proteins for interaction. The method can be used to screen a protein library for interactions with a bait protein or to test for association between proteins that are expected to interact based on prior evidence. Interaction mating facilitates the screening of a library with multiple bait proteins.
authors.affiliation: Fox Chase Cancer Center, Philadelphia, Pennsylvania, USA. EA Golemis@fccc.edu
authors.forename: Erica A
authors.initials: EA
authors.lastname: Golemis
authors.forename: Ilya
authors.initials: I
authors.lastname: Serebriiskii
authors.forename: Russell L
authors.initials: RL
authors.lastname: Finley
authors.forename: Mikhail G
authors.initials: MG
authors.lastname: Kolonin
authors.forename: Jeno
authors.initials: J
authors.lastname: Gyuris
authors.forename: Roger
authors.initials: R
authors.lastname: Brent
mesh_terms: D000818:Animals; D002417:Cattle; D018929:Cell Culture Techniques / Q000379:methods; D045744:Cell Line, Tumor; D004331:Drosophila melanogaster; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D019169:Jurkat Cells; D051379:Mice; D010957:Plasmids / Q000235:genetics; D025941:Protein Interaction Mapping / Q000379:methods*; D040901:Proteomics / Q000379:methods*; D054852:Small Molecule Libraries; D015533:Transcriptional Activation / Q000235:genetics; D014170:Transformation, Genetic / Q000502:physiology; D020798:Two-Hybrid System Techniques / Q000592:standards*; D015003:Yeasts / Q000235:genetics*
chemical_list: D005656:Fungal Proteins; D054852:Small Molecule Libraries