title: A RAPD-PCR method for the rapid detection of Bacillus cereus.
abstract: Distinction of Bacillus cereus from other closely related bacilli is challenging and new efficient methods are continually demanded. From our previous work on RAPD profiles of bacilli, we found a possibility that B. cereus strains could be distinguished from other bacilli. In this work, RAPD-PCR profiles of B. cereus strains were obtained using a 10-mer (S30) as a primer, and a B. cereus specific 0.91-kb band was produced from all tested strains. The RAPD-PCR procedure also successfully detected B. cereus from spiked cheonggukjang when B. cereus cells were present at more than 10(2)/g sample.
authors.affiliation: Division of Applied Life Science (BK21), Graduate School, Gyeongsang National University, Jinju, Korea.
authors.forename: Jiyeon
authors.initials: J
authors.lastname: Lee
authors.forename: Gun-Hee
authors.initials: GH
authors.lastname: Kwon
authors.forename: Jae-Yong
authors.initials: JY
authors.lastname: Park
authors.forename: Cheon-Seok
authors.initials: CS
authors.lastname: Park
authors.forename: Dae Young
authors.initials: DY
authors.lastname: Kwon
authors.forename: Jinkyu
authors.initials: J
authors.lastname: Lim
authors.forename: Jong Sang
authors.initials: JS
authors.lastname: Kim
authors.forename: Jeong Hwan
authors.initials: JH
authors.lastname: Kim
mesh_terms: D001409:Bacillus cereus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D001431:Bacteriological Techniques / Q000379:methods*; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D012680:Sensitivity and Specificity