title: Differentiation of foot-and-mouth disease-infected pigs from vaccinated pigs using antibody-detecting sandwich ELISA.
abstract: The presence of serum antibodies for nonstructural proteins of the foot-and-mouth disease virus (FMDV) can differentiate FMDV-infected animals from vaccinated animals. In this study, a sandwich ELISA was developed for rapid detection of the foot-and-mouth disease (FMD) antibodies; it was based on an Escherichia coli-expressed, highly conserved region of the 3ABC nonstructural protein of the FMDV O/TW/99 strain and a monoclonal antibody derived from the expressed protein. The diagnostic sensitivity of the assay was 98.4%, and the diagnostic specificity was 100% for naïve and vaccinated pigs; the detection ability of the assay was comparable those of the PrioCHECK and UBI kits. There was 97.5, 93.4 and 66.6% agreement between the results obtained from our ELISA and those obtained from the PrioCHECK, UBI and CHEKIT kits, respectively. The kappa statistics were 0.95, 0.87 and 0.37, respectively. Moreover, antibodies for nonstructural proteins of the serotypes A, C, Asia 1, SAT 1, SAT 2 and SAT 3 were also detected in bovine sera. Furthermore, the absence of cross-reactions generated by different antibody titers against the swine vesicular disease virus and vesicular stomatitis virus (VSV) was also highlighted in this assay's specificity.
authors.affiliation: Division of Hog Cholera Research, Animal Health Research Institute, 376 Chung-Cheng Road, Tamsui, New Taipei City 25158, Taiwan.
authors.forename: Tsu-Han
authors.initials: TH
authors.lastname: Chen
authors.forename: Fan
authors.initials: F
authors.lastname: Lee
authors.forename: Yeou-Liang
authors.initials: YL
authors.lastname: Lin
authors.forename: Aldo
authors.initials: A
authors.lastname: Dekker
authors.forename: Wen-Bin
authors.initials: WB
authors.lastname: Chung
authors.forename: Chu-Hsiang
authors.initials: CH
authors.lastname: Pan
authors.forename: Ming-Hwa
authors.initials: MH
authors.lastname: Jong
authors.forename: Chin-Cheng
authors.initials: CC
authors.lastname: Huang
authors.forename: Ming-Chang
authors.initials: MC
authors.lastname: Lee
authors.forename: Hsiang-Jung
authors.initials: HJ
authors.lastname: Tsai
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D005536:Foot-and-Mouth Disease / Q000175:diagnosis* / Q000517:prevention & control; D029544:Foot-and-Mouth Disease Virus / Q000276:immunology; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000517:prevention & control; D014611:Vaccination / Q000662:veterinary*; D017361:Viral Nonstructural Proteins / Q000097:blood
chemical_list: C109754:3ABC protein, virus; D000914:Antibodies, Viral; D017361:Viral Nonstructural Proteins