title: Genotyping of white spot syndrome virus in Chinese cultured shrimp during 1998-1999.
abstract: Recent studies showed that white spot syndrome virus (WSSV) isolates from different geographic locations share a high genetic similarity except the variable regions in ORF23/24 and ORF14/15, and variable number of tandem repeats (VNTR) within ORF94. In this study, genotyping was performed according to these three variable regions among WSSV isolates collected during 1998/1999 from Southern China. These WSSV isolates contain a deletion of 1168, 5657, 5898, 9316 and 11093 bp, respectively in the variable region ORF23/24 compared with WSSV-TW, and a deletion of 4749 or 5622 bp in the variable region ORF14/15 relative to TH-96-II. Four types of repeat units (RUs) (6, 8, 9 and 13 RUs) in ORF94 were detected in these isolates, with the shortest 6 RUs as the most prevalent type. Our results provide important information for a better understanding of the spatio-temporal transmission mode and the WSSV genetic evolution lineage.
authors.affiliation: Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China. tan@nbio.uni-heidelberg.de
authors.forename: Yan-wei
authors.initials: YW
authors.lastname: Tan
authors.forename: Zheng-li
authors.initials: ZL
authors.lastname: Shi
mesh_terms: D000818:Animals; D002681:China; D014644:Genetic Variation; D005838:Genotype; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D033561:Penaeidae / Q000254:growth & development / Q000821:virology*; D046848:White spot syndrome virus 1 / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*