title: Functional phosphosite screening for targeted protein-protein interactions by combining phosphoproteomics strategies and mammalian two-hybrid assays.
abstract: An effective new platform for phosphosite mapping and subsequent functional screening was developed to analyze the targeted protein-protein interactions of p300 and CBP with β-catenin. Two novel functional phosphosites, Ser12 of p300 and Ser92 of CBP, were revealed to modulate p300/β-catenin and CBP/β-catenin interactions, respectively.
authors.affiliation: Keck School of Medicine, University of Southern California, 1501 San Pablo St., Los Angeles, CA 90033, USA. mingquag@usc.edu.
authors.forename: Mingquan
authors.initials: M
authors.lastname: Guo
authors.forename: Zanxian
authors.initials: Z
authors.lastname: Xia
authors.forename: Hong
authors.initials: H
authors.lastname: Ma
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D050882:CREB-Binding Protein / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D002460:Cell Line; D002853:Chromatography, Liquid; D006801:Humans; D008969:Molecular Sequence Data; D046912:Multiprotein Complexes / Q000737:chemistry; D010750:Phosphoproteins / Q000737:chemistry*; D010766:Phosphorylation; D020543:Proteome / Q000737:chemistry*; D040901:Proteomics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D020539:Sequence Analysis, Protein; D053719:Tandem Mass Spectrometry; D020798:Two-Hybrid System Techniques; D051176:beta Catenin / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D050880:p300-CBP Transcription Factors / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D046912:Multiprotein Complexes; D010750:Phosphoproteins; D020543:Proteome; D011994:Recombinant Proteins; D051176:beta Catenin; D050882:CREB-Binding Protein; D050880:p300-CBP Transcription Factors