title: [The replication capacity and drug sensitivity of Adefovir dipivoxil-resistant HBV mutants in vivo].
abstract: This study was aimed to research the changes of the biological characteristics of Hepatitis B virus-resistant mutant model in vivo. The anti-ADV mutant HBV plasmid of rtA181V and rtN236T, prepared by Multi Site-Directed Mutagenesis Kit, was transferred into mice via the tail vein, and the levels of HBV-DNA replication were detected after Anti-HBV drugs treatment. The HBV-DNA replication had been detected in the mutant mice, which means that the establishment of HBV-resistance mutant model in vivo was successful, but the level of HBV DNA replication intermediates in the anti-ADV mutant mice liver were decreased compared with wild 4.1kb HBV plasmid mice. The wild-type mice were sensitive to Lamivudine, Adefovir dipivoxil and Entecavir. Though the mutant mice were also sensitive to Entecavir, the sensitivity to lamivudine and Adefovir dipivoxil decreased. As a result, this study established the Adefovir-resistant hepatitis B virus mouse model successfully. However, the replication level of HBV-DNA was reduced in this model, and Adefovir dipivoxil in the mutant mice had no significant inhibition effect on HBV-DNA.
authors.affiliation: The Center of Infectious Diseases, West China Hospital of Sichuan University, Division of Infectious Diseases, State Key Laboratory of Biotherapy, Sichuan University, Chengdu 610041, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Lei
authors.forename: Zhan
authors.initials: Z
authors.lastname: Gao
authors.forename: Lu
authors.initials: L
authors.lastname: Xu
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.forename: Hong
authors.initials: H
authors.lastname: Tang
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology; D001483:Base Sequence; D024882:Drug Resistance, Viral / Q000235:genetics*; D006509:Hepatitis B / Q000188:drug therapy / Q000821:virology; D006515:Hepatitis B virus / Q000187:drug effects* / Q000235:genetics*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D063065:Organophosphonates / Q000494:pharmacology*
chemical_list: D000998:Antiviral Agents; D063065:Organophosphonates; D000225:Adenine; C106812:adefovir dipivoxil