title: Relationship between depression anxiety stress scale (DASS) and urinary hydroxyproline and proline concentrations in hospital workers.
abstract: OBJECTIVE
  Although increased reactive oxygen species (ROS) is caused by stress accelerates collagen degradation, there was no data on the relationship between stress and urinary hydroxyproline (Hyp) and proline (Pro), a good marker of collagen degradation. The purpose of this study was to evaluate the relationship between depression, anxiety, and stress (DAS) and concentrations of urinary Hyp and Pro.
  
  
  METHODS
  97 hospital employees aged 20 to 58 were asked to fill out comprehensive self-administrated questionnaires containing information about their medical history, lifestyle, length of the work year, shift-work and DAS. depression anxiety stress scale (DASS) was applied to evaluate chronic mental disorders. Urine samples were analyzed using high performance liquid chromatography (HPLC) with double derivatization for the assay of hydroxyproline and proline.
  
  
  RESULTS
  The mean value of Hyp and Pro concentration in all subjects was 194.1 ± 113.4 μmol/g and 568.2 ± 310.7 μmol/g. DASS values and urinary Pro concentrations were differentiated by sex (female > male, p < 0.05) and type of job (nurse > others, p < 0.05). In the stepwise multiple linear regressions, urinary Hyp and Pro concentrations were influenced by stress (Adjusted r2 = 0.051) and anxiety and job (Adjusted r2 = 0.199), respectively.
  
  
  CONCLUSIONS
  We found that stress and anxiety were correlated with urinary Hyp and Pro concentrations. To identifying a definite correlation, further study in large populations will be needed.
authors.affiliation: Department of Occupational and Environmental Medicine, Ajou University Medical Center, Suwon, Korea.
authors.forename: Keou Won
authors.initials: KW
authors.lastname: Lee
authors.forename: Soo Jeong
authors.initials: SJ
authors.lastname: Kim
authors.forename: Jae Beom
authors.initials: JB
authors.lastname: Park
authors.forename: Kyung Jong
authors.initials: KJ
authors.lastname: Lee
mesh_terms: D000328:Adult; D001007:Anxiety / Q000652:urine*; D003863:Depression / Q000652:urine*; D005260:Female; D006801:Humans; D006909:Hydroxyproline / Q000652:urine; D008297:Male; D008875:Middle Aged; D010564:Personnel, Hospital / Q000523:psychology*; D011392:Proline / Q000652:urine*; D056910:Republic of Korea; D013315:Stress, Psychological / Q000652:urine*; D011795:Surveys and Questionnaires; D055815:Young Adult
chemical_list: D011392:Proline; D006909:Hydroxyproline