title: Transmembrane pores formed by human antimicrobial peptide LL-37.
abstract: Human LL-37 is a multifunctional cathelicidin peptide that has shown a wide spectrum of antimicrobial activity by permeabilizing microbial membranes similar to other antimicrobial peptides; however, its molecular mechanism has not been clarified. Two independent experiments revealed LL-37 bound to membranes in the α-helical form with the axis lying in the plane of membrane. This led to the conclusion that membrane permeabilization by LL-37 is a nonpore carpet-like mechanism of action. Here we report the detection of transmembrane pores induced by LL-37. The pore formation coincided with LL-37 helices aligning approximately normal to the plane of the membrane. We observed an unusual phenomenon of LL-37 embedded in stacked membranes, which are commonly used in peptide orientation studies. The membrane-bound LL-37 was found in the normal orientation only when the membrane spacing in the multilayers exceeded its fully hydrated value. This was achieved by swelling the stacked membranes with excessive water to a swollen state. The transmembrane pores were detected and investigated in swollen states by means of oriented circular dichroism, neutron in-plane scattering, and x-ray lamellar diffraction. The results are consistent with the effect of LL-37 on giant unilamellar vesicles. The detected pores had a water channel of radius 23-33 Å. The molecular mechanism of pore formation by LL-37 is consistent with the two-state model exhibited by magainin and other small pore-forming peptides. The discovery that peptide-membrane interactions in swollen states are different from those in less hydrated states may have implications for other large membrane-active peptides and proteins studied in stacked membranes.
authors.affiliation: Department of Physics and Astronomy, Rice University, Houston, Texas, USA.
authors.forename: Chang-Chun
authors.initials: CC
authors.lastname: Lee
authors.forename: Yen
authors.initials: Y
authors.lastname: Sun
authors.forename: Shuo
authors.initials: S
authors.lastname: Qian
authors.forename: Huey W
authors.initials: HW
authors.lastname: Huang
mesh_terms: D000595:Amino Acid Sequence; D023181:Antimicrobial Cationic Peptides; D054804:Cathelicidins / Q000737:chemistry / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D002942:Circular Dichroism; D006801:Humans; D008969:Molecular Sequence Data; D033363:Neutron Diffraction; D009502:Neutrons; D016062:Porosity; D011485:Protein Binding; D053835:Unilamellar Liposomes / Q000378:metabolism; D014961:X-Ray Diffraction
chemical_list: D023181:Antimicrobial Cationic Peptides; D054804:Cathelicidins; D053835:Unilamellar Liposomes