title: Selective depletion of neuropathy-related antibodies from human serum by monolithic affinity columns containing ganglioside mimics.
abstract: Monolithic columns containing ganglioside GM2 and GM3 mimics were prepared for selective removal of serum anti-ganglioside antibodies from patients with acute and chronic immune-mediated neuropathies. ELISA results demonstrated that anti-GM2 IgM antibodies in human sera and a mouse monoclonal anti-GM2 antibody were specifically and selectively adsorbed by monolithic GM2 mimic columns and not by blank monolithic columns or monolithic GM3 mimic columns. In control studies, serum antibodies against the ganglioside GQ1b from another neuropathy patient were not depleted by monolithic GM2 mimic columns. Fluorescence microscopy with FITC-conjugated anti-human immunoglobulin antibodies showed that the immobilized ganglioside mimics were evenly distributed along the column. The columns were able to capture ∼95% of the anti-GM2 antibodies of patients after only 2 min of incubation. A monolithic column of 4.4 μL can deplete 28.2 μL of undiluted serum. These columns are potential diagnostic and therapeutic tools for neuropathies related to anti-ganglioside antibodies.
authors.affiliation: Laboratory of Organic Chemistry, Wageningen University, Dreijenplein 8, 6703 HB Wageningen, The Netherlands.
authors.forename: Kishore K R
authors.initials: KK
authors.lastname: Tetala
authors.forename: Astrid P
authors.initials: AP
authors.lastname: Heikema
authors.forename: Aliaksei V
authors.initials: AV
authors.lastname: Pukin
authors.forename: Carel A G M
authors.initials: CA
authors.lastname: Weijers
authors.forename: Anne P
authors.initials: AP
authors.lastname: Tio-Gillen
authors.forename: Michel
authors.initials: M
authors.lastname: Gilbert
authors.forename: Hubert P
authors.initials: HP
authors.lastname: Endtz
authors.forename: Alex
authors.initials: A
authors.lastname: van Belkum
authors.forename: Han
authors.initials: H
authors.lastname: Zuilhof
authors.forename: Gerben M
authors.initials: GM
authors.lastname: Visser
authors.forename: Bart C
authors.initials: BC
authors.lastname: Jacobs
authors.forename: Teris A
authors.initials: TA
authors.lastname: van Beek
mesh_terms: D000327:Adsorption; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D015195:Drug Design; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D016650:Fluorescein-5-isothiocyanate / Q000494:pharmacology; D005678:G(M2) Ganglioside / Q000737:chemistry; D005732:Gangliosides / Q000737:chemistry*; D006801:Humans; D007075:Immunoglobulin M / Q000737:chemistry; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods; D017933:Peripheral Nervous System / Q000473:pathology; D010523:Peripheral Nervous System Diseases / Q000188:drug therapy
chemical_list: D000911:Antibodies, Monoclonal; D005732:Gangliosides; D007075:Immunoglobulin M; D005678:G(M2) Ganglioside; C039129:GQ1b ganglioside; D016650:Fluorescein-5-isothiocyanate