title: Proteome analysis of differential protein expression in brain of rats with type 1 diabetes mellitus.
abstract: OBJECTIVE
  Proteomic approach was applied to identify differential protein expressed in brain of rats with type 1 diabetes mellitus (T1DM) in order to search for potential biomarkers for pathological changes of brain with T1DM.
  
  
  METHODS
  Proteins were extracted from brain tissues of T1DM rats and healthy control rats, separated by 2-DE and identified by MALDI-TOF-MS. The results were validated by Western blot analysis and immunohistochemistry (IHC).
  
  
  RESULTS
  A total of 8 proteins from the 24 differentially expressed spots were identified by MALDI-TOF-MS. The proteins identified were vitamin D-dependent calcium-binding protein, creatine kinase B-type (B-CK), myosin light chain kinase (MLCK), HSP60 and HSP71, ATP synthase, cyclin-G, pantothenate kinase-1 (PANK1), respectively. 3 proteins were up-regulated and 5 proteins were down-regulated from the T1DM rats. Of the 8 proteins identified, MLCK was confirmed by Western blot and IHC.
  
  
  CONCLUSIONS
  This work demonstrates that a comprehensive strategy of proteomic identification should be a useful tool for understanding of diabetic encephalopathy mechanism. And the differential proteins such as MLCK may be give clues about the pathogenesis of diabetic encephalopathy.
authors.affiliation: Department of Medical Laboratory, Guiyang Medical College, Guiyang, Guizhou, P.R. China.
authors.forename: X
authors.initials: X
authors.lastname: Li
authors.forename: W
authors.initials: W
authors.lastname: Pan
authors.forename: G Z
authors.initials: GZ
authors.lastname: Yang
authors.forename: Y N
authors.initials: YN
authors.lastname: Di
authors.forename: F
authors.initials: F
authors.lastname: Zhao
authors.forename: L Y
authors.initials: LY
authors.lastname: Zhu
authors.forename: Z H
authors.initials: ZH
authors.lastname: Jiang
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D001923:Brain Chemistry / Q000235:genetics / Q000502:physiology; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D015180:Electrophoresis, Gel, Two-Dimensional; D056726:Genetic Association Studies; D008297:Male; D020543:Proteome / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D054928:Validation Studies as Topic
chemical_list: D020543:Proteome