title: Quantitative proteomics of yeast post-Golgi vesicles reveals a discriminating role for Sro7p in protein secretion.
abstract: We here report the first comparative proteomics of purified yeast post-Golgi vesicles (PGVs). Vesicle samples isolated from PGV-accumulating sec6-4 mutants were treated with isobaric tags (iTRAQ) for subsequent quantitative tandem mass spectrometric analysis of protein content. After background subtraction, a total of 66 vesicle-associated proteins were identified, including known or assumed vesicle residents as well as a fraction not previously known to be PGV associated. Vesicles isolated from cells lacking the polarity protein Sro7p contained essentially the same catalogue of proteins but showed a reduced content of a subset of cargo proteins, in agreement with a previously shown selective role for Sro7p in cargo sorting.
authors.affiliation: Department of Cell and Molecular Biology, Microbiology, University of Gothenburg, Box 462, SE-405 30 Gothenburg, Sweden.
authors.forename: Annabelle
authors.initials: A
authors.lastname: Forsmark
authors.forename: Guendalina
authors.initials: G
authors.lastname: Rossi
authors.forename: Ingrid
authors.initials: I
authors.lastname: Wadskog
authors.forename: Patrick
authors.initials: P
authors.lastname: Brennwald
authors.forename: Jonas
authors.initials: J
authors.lastname: Warringer
authors.forename: Lennart
authors.initials: L
authors.lastname: Adler
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D022162:Cytoplasmic Vesicles / Q000737:chemistry* / Q000378:metabolism; D006056:Golgi Apparatus / Q000737:chemistry / Q000378:metabolism*; D021381:Protein Transport; D040901:Proteomics / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D015415:Biomarkers; D011993:Recombinant Fusion Proteins; C116430:SRO7 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins