title: Glycome diagnosis of human induced pluripotent stem cells using lectin microarray.
abstract: Induced pluripotent stem cells (iPSCs) can now be produced from various somatic cell (SC) lines by ectopic expression of the four transcription factors. Although the procedure has been demonstrated to induce global change in gene and microRNA expressions and even epigenetic modification, it remains largely unknown how this transcription factor-induced reprogramming affects the total glycan repertoire expressed on the cells. Here we performed a comprehensive glycan analysis using 114 types of human iPSCs generated from five different SCs and compared their glycomes with those of human embryonic stem cells (ESCs; nine cell types) using a high density lectin microarray. In unsupervised cluster analysis of the results obtained by lectin microarray, both undifferentiated iPSCs and ESCs were clustered as one large group. However, they were clearly separated from the group of differentiated SCs, whereas all of the four SCs had apparently distinct glycome profiles from one another, demonstrating that SCs with originally distinct glycan profiles have acquired those similar to ESCs upon induction of pluripotency. Thirty-eight lectins discriminating between SCs and iPSCs/ESCs were statistically selected, and characteristic features of the pluripotent state were then obtained at the level of the cellular glycome. The expression profiles of relevant glycosyltransferase genes agreed well with the results obtained by lectin microarray. Among the 38 lectins, rBC2LCN was found to detect only undifferentiated iPSCs/ESCs and not differentiated SCs. Hence, the high density lectin microarray has proved to be valid for not only comprehensive analysis of glycans but also diagnosis of stem cells under the concept of the cellular glycome.
authors.affiliation: From the Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology, 1-1-1 Umezono, Tsukuba, Ibaraki 305-8568, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Tateno
authors.forename: Masashi
authors.initials: M
authors.lastname: Toyota
authors.forename: Shigeru
authors.initials: S
authors.lastname: Saito
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Onuma
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Ito
authors.forename: Keiko
authors.initials: K
authors.lastname: Hiemori
authors.forename: Mihoko
authors.initials: M
authors.lastname: Fukumura
authors.forename: Asako
authors.initials: A
authors.lastname: Matsushima
authors.forename: Mio
authors.initials: M
authors.lastname: Nakanishi
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Ohnuma
authors.forename: Hidenori
authors.initials: H
authors.lastname: Akutsu
authors.forename: Akihiro
authors.initials: A
authors.lastname: Umezawa
authors.forename: Katsuhisa
authors.initials: K
authors.lastname: Horimoto
authors.forename: Jun
authors.initials: J
authors.lastname: Hirabayashi
authors.forename: Makoto
authors.initials: M
authors.lastname: Asashima
mesh_terms: D054794:Glycomics / Q000379:methods*; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism*; D037102:Lectins / Q000737:chemistry*; D011134:Polysaccharides / Q000235:genetics / Q000378:metabolism*; D040081:Protein Array Analysis / Q000379:methods*
chemical_list: D037102:Lectins; D011134:Polysaccharides