title: Molecular analysis of common wheat genes encoding three types of cytosolic heat shock protein 90 (Hsp90): functional involvement of cytosolic Hsp90s in the control of wheat seedling growth and disease resistance.
abstract: Heat shock protein 90 (Hsp90) molecular chaperones play important roles in plant growth and responses to environmental stimuli. However, little is known about the genes encoding Hsp90s in common wheat. Here, we report genetic and functional analysis of the genes specifying cytosolic Hsp90s in this species. Three groups of homoeologous genes (TaHsp90.1, TaHsp90.2 and TaHsp90.3), encoding three types of cytosolic Hsp90, were isolated. The loci containing TaHsp90.1, TaHsp90.2 and TaHsp90.3 genes were assigned to groups 2, 7 and 5 chromosomes, respectively. TaHsp90.1 genes exhibited higher transcript levels in the stamen than in the leaf, root and culm. TaHsp90.2 and TaHsp90.3 genes were more ubiquitously transcribed in the vegetative and reproductive organs examined. Decreasing the expression of TaHsp90.1 genes through virus-induced gene silencing (VIGS) caused pronounced inhibition of wheat seedling growth, whereas the suppression of TaHsp90.2 or TaHsp90.3 genes via VIGS compromised the hypersensitive resistance response of the wheat variety Suwon 11 to stripe rust fungus. Our work represents the first systematic determination of wheat genes encoding cytosolic Hsp90s, and provides useful evidence for the functional involvement of cytosolic Hsp90s in the control of seedling growth and disease resistance in common wheat.
authors.affiliation: The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.|Graduate School of Chinese Academy of Sciences, Yuquan Road, Beijing 100039, China.
authors.forename: Guan-Feng
authors.initials: GF
authors.lastname: Wang
authors.affiliation: The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.|Graduate School of Chinese Academy of Sciences, Yuquan Road, Beijing 100039, China.
authors.forename: Xuening
authors.initials: X
authors.lastname: Wei
authors.affiliation: The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Renchun
authors.initials: R
authors.lastname: Fan
authors.affiliation: The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.|Graduate School of Chinese Academy of Sciences, Yuquan Road, Beijing 100039, China.
authors.forename: Huanbin
authors.initials: H
authors.lastname: Zhou
authors.affiliation: The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Xianping
authors.initials: X
authors.lastname: Wang
authors.affiliation: The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.|Graduate School of Chinese Academy of Sciences, Yuquan Road, Beijing 100039, China.
authors.forename: Chunmei
authors.initials: C
authors.lastname: Yu
authors.affiliation: The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.|Graduate School of Chinese Academy of Sciences, Yuquan Road, Beijing 100039, China.
authors.forename: Lingli
authors.initials: L
authors.lastname: Dong
authors.affiliation: The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Zhenying
authors.initials: Z
authors.lastname: Dong
authors.affiliation: College of Plant Protection and Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling 712100, China.
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Plant Protection and Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling 712100, China.
authors.forename: Zhensheng
authors.initials: Z
authors.lastname: Kang
authors.affiliation: The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Hongqing
authors.initials: H
authors.lastname: Ling
authors.affiliation: The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Qian-Hua
authors.initials: QH
authors.lastname: Shen
authors.affiliation: The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Daowen
authors.initials: D
authors.lastname: Wang
authors.affiliation: The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Xiangqi
authors.initials: X
authors.lastname: Zhang
mesh_terms: D001487:Basidiomycota / Q000502:physiology*; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics; D003600:Cytosol / Q000737:chemistry; D018076:DNA, Complementary; D005091:Exons / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D055785:Gene Knockdown Techniques; D020868:Gene Silencing; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D007438:Introns / Q000235:genetics; D009928:Organ Specificity / Q000235:genetics; D010641:Phenotype; D010802:Phylogeny; D010935:Plant Diseases / Q000276:immunology* / Q000382:microbiology; D057865:Plant Immunity / Q000276:immunology*; D018515:Plant Leaves / Q000276:immunology / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D036226:Seedlings / Q000254:growth & development / Q000276:immunology / Q000382:microbiology; D014908:Triticum / Q000235:genetics / Q000276:immunology / Q000382:microbiology / Q000502:physiology*
chemical_list: D018076:DNA, Complementary; D018841:HSP90 Heat-Shock Proteins; D010940:Plant Proteins