title: Multiresidue method to quantify pesticides in fish muscle by QuEChERS-based extraction and LC-MS/MS.
abstract: Pesticide residues in fish muscle are an environmental and a health safety concern which requires analytical methods presenting high sensitivity and low limits of quantification. In this study, adapted QuEChERS method, coupled to liquid chromatography tandem mass spectrometry (Scheduled MRM-5500 QTRAP), was developed to quantify 13 pesticides (azoxystrobin, clomazone, diflufenican, dimethachlor, carbendazim, iprodion, isoproturon, mesosulfuron-methyl, metazachlor, napropamid, quizalofop and thifensulfuron-methyl) in muscle of fish. Quantification limits were below 1 ng g(-1) except for clomazone (1.8 ng g(-1)) and quizalofop (7.4 ng g(-1)). Best recoveries were observed for perch (>80%) and roach (>68%), except for thifensulfuron-methyl. Lower recoveries had been observed for carp (6% to 86%). Relative standard deviation was lower than 28% for intra-day and 29% for inter-day analysis, respectively. This method was successfully tested on three fish species, naturally or orally exposed: roach (Rutilus rutilus), perch (Perca fluviatilis) and carp (Cyprinus carpio). Few levels were observed in fish naturally exposed, but carp and perch orally contaminated showed measurable levels in their muscles.
authors.affiliation: Unité de Recherche Animal et Fonctionnalité des Produits Animaux, Nancy Université, INRA, 2 avenue de la Forêt de Haye, BP 172, 54505, Vandœuvre-lès-Nancy, France.
authors.forename: Angélique
authors.initials: A
authors.lastname: Lazartigues
authors.forename: Laure
authors.initials: L
authors.lastname: Wiest
authors.forename: Robert
authors.initials: R
authors.lastname: Baudot
authors.forename: Marielle
authors.initials: M
authors.lastname: Thomas
authors.forename: Cyril
authors.initials: C
authors.lastname: Feidt
authors.forename: Cécile
authors.initials: C
authors.lastname: Cren-Olivé
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D005399:Fishes; D009132:Muscles / Q000737:chemistry*; D010573:Pesticide Residues / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D010573:Pesticide Residues