title: BTEB2 knockdown suppresses neointimal hyperplasia in a rat artery balloon injury model.
abstract: Basic transcription element-binding protein 2 (BTEB2) is a regulator of the proliferation and phenotypic changes of vascular smooth muscle cells (SMCs). The aim of the present study was to determine whether or not BTEB2 knockdown inhibits balloon injury-induced neointimal hyperplasia attributed to the proliferation and phenotypic changes of vascular SMCs. We found that the knockdown of BTEB2 with antisense oligonucleotides (Ad-As-BTEB2) significantly reduced the intima/media ratio compared to uninjured arteries and vessels treated with Ad-LacZ. Knockdown of BTEB2 suppresses the proliferation of cultured vascular SMCs, concurrent with the down-regulation of proliferating cell nuclear antigen, angiotensin II type 1 receptor and platelet-derived growth factor BB. In addition, BTEB2 knockdown caused the up-regulation of the differentiation marker smooth muscle α-actin and down-regulation of the dedifferentiation marker embryonic smooth muscle myosin heavy chain. The present study provides direct evidence that BTEB2 plays a critical role in balloon injury-induced neointimal hyperplasia, which is closely linked to vascular SMC proliferation and phenotypic modulation. This study highlights the fact that BTEB2 may be a potential target for the prevention of restenosis after vascular intervention.
authors.affiliation: Department of Cardiology, General Hospital of PLA Chengdu Military Area Command, Sichuan 610083, PR China.
authors.forename: De
authors.initials: D
authors.lastname: Li
authors.forename: Shuangtao
authors.initials: S
authors.lastname: Ma
authors.forename: Yongjian
authors.initials: Y
authors.lastname: Yang
authors.forename: Dachun
authors.initials: D
authors.lastname: Yang
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Zhu
authors.forename: Meiqin
authors.initials: M
authors.lastname: Sun
authors.forename: Bing
authors.initials: B
authors.lastname: Tang
mesh_terms: D000818:Animals; D002339:Carotid Arteries / Q000378:metabolism / Q000473:pathology; D002404:Catheterization / Q000009:adverse effects*; D049109:Cell Proliferation; D004195:Disease Models, Animal; D055785:Gene Knockdown Techniques; D006801:Humans; D006965:Hyperplasia; D051741:Kruppel-Like Transcription Factors / Q000378:metabolism*; D008297:Male; D051379:Mice; D032389:Myocytes, Smooth Muscle / Q000378:metabolism / Q000473:pathology; D010641:Phenotype; D051381:Rats; D017208:Rats, Wistar; D017539:Tunica Intima / Q000378:metabolism* / Q000473:pathology*
chemical_list: C118391:Klf5 protein, mouse; C492215:Klf5 protein, rat; D051741:Kruppel-Like Transcription Factors