title: Modulation of human immune responses by bovine interleukin-10.
abstract: Cytokines can be functionally active across species barriers. Bovine IL-10 has an amino acid sequence identity with human IL-10 of 76.8%. Therefore, the aim of this study was to evaluate whether bovine IL-10 has immunomodulatory activities on human monocytes and dendritic cells. Peripheral blood monocytes were isolated from healthy donors, and used directly or allowed to differentiate to dendritic cells under the influence of IL-4 and GM-CSF. Recombinant bovine IL-10 inhibited TLR induced activation of monocytes, and dose-dependently inhibited LPS-induced activation of monocyte-derived DCs comparable to human IL-10. By using blocking antibodies to either bovine IL-10 or the human IL-10 receptor it was demonstrated that inhibition of monocyte activation by bovine IL-10 was dependent on binding of bovine IL-10 to the human IL-10R. These data demonstrate that bovine IL-10 potently inhibits the activation of human myeloid cells in response to TLR activation. Bovine IL-10 present in dairy products may thus potentially contribute to the prevention of necrotizing enterocolitis and allergy, enhance mucosal tolerance induction and decrease intestinal inflammation and may therefore be applicable in infant foods and in immunomodulatory diets.
authors.affiliation: Cell Biology and Immunology Group, Wageningen University, Wageningen, The Netherlands.
authors.forename: Gerco
authors.initials: G
authors.lastname: den Hartog
authors.forename: Huub F J
authors.initials: HF
authors.lastname: Savelkoul
authors.forename: Ruud
authors.initials: R
authors.lastname: Schoemaker
authors.forename: Edwin
authors.initials: E
authors.lastname: Tijhaar
authors.forename: Adrie H
authors.initials: AH
authors.lastname: Westphal
authors.forename: Talitha
authors.initials: T
authors.lastname: de Ruiter
authors.forename: Elise
authors.initials: E
authors.lastname: van de Weg-Schrijver
authors.forename: R J Joost
authors.initials: RJ
authors.lastname: van Neerven
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution / Q000235:genetics; D000818:Animals; D001665:Binding Sites; D001682:Biological Availability; D015415:Biomarkers / Q000378:metabolism; D002417:Cattle; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007109:Immunity / Q000187:drug effects*; D016753:Interleukin-10 / Q000737:chemistry / Q000494:pharmacology*; D008024:Ligands; D008070:Lipopolysaccharides / Q000494:pharmacology; D008969:Molecular Sequence Data; D009000:Monocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D053701:Receptors, Interleukin-10 / Q000737:chemistry / Q000378:metabolism; D051193:Toll-Like Receptors / Q000378:metabolism
chemical_list: D015415:Biomarkers; D008024:Ligands; D008070:Lipopolysaccharides; D053701:Receptors, Interleukin-10; D051193:Toll-Like Receptors; D016753:Interleukin-10