title: High-throughput approaches for plant epigenomic studies.
abstract: In plant cells, DNA is packaged into chromatin by wrapping around histone octamers. Pathways that lead to cytosine DNA methylation, posttranslational histone modifications and certain components of the RNA interfering (RNAi) pathway are critically important in modulating chromatin structure, thereby affecting many molecular processes that take place in a cell. Recent advances in microarray and high-throughput sequencing technologies have made it possible to study these pathways on a genome-wide scale. Results from such epigenomic studies are broadening our understanding of plant genomes and are also providing important clues regarding the mechanisms and functions of these pathways that can be further tested using genetic and biochemical approaches. This review focuses on the high-throughput approaches that have been successfully applied in plant epigenomic studies.
authors.affiliation: Plant Biology and Genomic Analysis Laboratories, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Schmitz
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Zhang
mesh_terms: D019175:DNA Methylation; D044127:Epigenesis, Genetic; D057890:Epigenomics / Q000379:methods*; D020869:Gene Expression Profiling; D018745:Genome, Plant; D059014:High-Throughput Nucleotide Sequencing; D006657:Histones / Q000378:metabolism; D010944:Plants / Q000235:genetics*; D034622:RNA Interference; D017422:Sequence Analysis, DNA
chemical_list: D006657:Histones