title: Both swine and human cells are capable to support the replication of swine hepatitis E virus type 4 in vitro.
abstract: Using swine anal swab or liver as inocula, cell-culture systems were developed for swine hepatitis E virus (HEV) in swine cells (IBRS-2) and human cells (A549). Both positive and negative strand of swine HEV RNA were detected continuously. Cytopathic effect appeared from passage 8 in IBRS-2 and passage 22 in A549. Viral antigen was detected by indirect immunofluorescent assay in infected cells. Progenies harbored mutations in the third nucleotide of codon. Amino acid changes occurred in passage 8 in IBRS-2 and rescued in passage 10. Full-length genome sequence of a swine HEV isolate from liver was determined to be genotype 4. Taken together, our data suggest that swine HEV is able to replicate in both swine and human cells in vitro.
authors.affiliation: Division of Animal Infectious Diseases, State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
authors.forename: Hou-Yong
authors.initials: HY
authors.lastname: Zhang
authors.forename: Dong-Sheng
authors.initials: DS
authors.lastname: Chen
authors.forename: Yi-Quan
authors.initials: YQ
authors.lastname: Wu
authors.forename: Qi-Gai
authors.initials: QG
authors.lastname: He
authors.forename: Huan-Chun
authors.initials: HC
authors.lastname: Chen
authors.forename: Zheng-Fei
authors.initials: ZF
authors.lastname: Liu
mesh_terms: D000220:Adaptation, Biological; D019943:Amino Acid Substitution / Q000235:genetics; D001003:Anal Canal / Q000821:virology; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D018929:Cell Culture Techniques; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D019085:Fluorescent Antibody Technique, Direct; D016751:Hepatitis E / Q000662:veterinary* / Q000821:virology; D016752:Hepatitis E virus / Q000254:growth & development*; D006801:Humans; D008099:Liver / Q000821:virology; D008969:Molecular Sequence Data; D020125:Mutation, Missense; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D013552:Swine; D013553:Swine Diseases / Q000821:virology
chemical_list: D000956:Antigens, Viral; D012367:RNA, Viral