title: COI-based DNA barcoding of Arcoida species (Bivalvia: Pteriomorphia) along the coast of China.
abstract: DNA barcoding is a promising tool for the rapid and unambiguous identification of species. Some arcoid species are particularly difficult to distinguish with traditional morphological identification owing to phenotypic variation and the existence of closely related taxa. Here, we apply DNA barcoding based on mitochondrial cytochrome c oxidase I gene (COI) to arcoid species collected from the coast along China. Combining morphology with molecular data indicates the 133 specimens of Arcoida could be assigned to 24 species. Because of the deep genetic divergence within Tegillarca granosa, there was an overlap between genetic variation within species and variation between species. Nevertheless, NJ and Bayesian trees showed that all species fell into reciprocally monophyletic clades with high bootstrap values. Our results evidence that the COI marker can efficiently identify species, correct mistakes caused by morphological identification and reveal genetic differentiation among populations within species. This study provides a clear example of the usefulness of barcoding for arcoid identification. Furthermore, it also lays a foundation for other biological and ecological studies of Arcoida.
authors.affiliation: Key Laboratory of Mariculture Ministry of Education, Ocean University of China, Qingdao 266003, China.
authors.forename: Yanwei
authors.initials: Y
authors.lastname: Feng
authors.forename: Qi
authors.initials: Q
authors.lastname: Li
authors.forename: Lingfeng
authors.initials: L
authors.lastname: Kong
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Zheng
mesh_terms: D000818:Animals; D049872:Bivalvia / Q000033:anatomy & histology / Q000145:classification* / Q000235:genetics*; D002681:China; D058893:DNA Barcoding, Taxonomic / Q000379:methods*; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
chemical_list: D004272:DNA, Mitochondrial; D003576:Electron Transport Complex IV