title: Complete nucleotide sequence of Croton yellow vein mosaic virus and DNA-β associated with yellow vein mosaic disease of Jatropha gossypifolia in India.
abstract: A severe yellow vein mosaic disease was noticed on several Jatropha gossypifolia plants growing nearby agriculture fields at Lucknow, India. Diseased plants exhibited yellow vein mosaic, leaf deformation, vein swelling and stunting. A population of whiteflies (Bemisia tabaci) was also noticed in the vicinities; therefore, begomovirus infection was suspected. To confirm begomovirus association, total DNA was isolated from symptomatic leaf samples and subjected to PCR using DNA-A, DNA-B and DNA-β-specific primers. DNA-A and DNA-β was successfully amplified but several attempts failed to amplify DNA-B indicating monopartite nature of the begomovirus. The sequence analysis of amplicons revealed the presence of 2757 nucleotides of DNA-A genome (EU727086) and 1315 nt of DNA-β molecule (EU604296). The sequence analysis of DNA-A (EU727086) revealed the highest 96% identities and closest relationship with Croton yellow vein mosaic virus (CYVMV, AJ507777) infecting Croton bonplandianum in India. The DNA-β (EU604296) showed the highest 96% sequence identity and closest phylogenetic relationship with CYVMV-associated DNA-β (AM410551) isolated from Croton sp. in Pakistan. Based on the highest sequence identities and closest phylogenetic relationships of the DNA-A genome and DNA-β molecule with respective sequences of various isolates of Croton yellow vein mosaic virus, the begomovirus associated with yellow vein mosaic disease of J. gossypifolia was identified as an isolate of Croton yellow vein mosaic virus.
authors.affiliation: Department of Plant Molecular Virology, CSIR-National Botanical Research Institute, Lucknow, 226 001, Uttar Pradesh, India.
authors.forename: S K
authors.initials: SK
authors.lastname: Snehi
authors.forename: M S
authors.initials: MS
authors.lastname: Khan
authors.forename: S K
authors.initials: SK
authors.lastname: Raj
authors.forename: V
authors.initials: V
authors.lastname: Prasad
mesh_terms: D000818:Animals; D053839:Begomovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D016000:Cluster Analysis; D004279:DNA, Viral / Q000235:genetics*; D016679:Genome, Viral; D006430:Hemiptera / Q000254:growth & development; D007194:India; D031286:Jatropha / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004279:DNA, Viral