title: Evaluation of non-aqueous capillary zone electrophoresis for the determination of histamine H2 receptor antagonists in pharmaceuticals.
abstract: A new non-aqueous capillary electrophoresis method is proposed for the separation and simultaneous determination of cimetidine, ranitidine, roxatidine, nizatidine and famotidine by using a 30-cm long × 75 µm i.d. fused silica capillary and UV detection at 214 nm. Using a temperature of 25°C, an applied voltage of 15 kV, and a background electrolyte consisting of methanol containing 10 mM ammonium acetate and 0.2% acetic acid allowed the analytes to be separated in less than 4 min. The limits of detection obtained ranged from 7 and 17 µg L(-1). The proposed method was successfully used to determine the analytes in pharmaceutical preparations and its results were checked against an HPLC method.
authors.affiliation: Departamento de Química Analítica y Tecnología de los Alimentos, Universidad de Castilla-La Mancha, Ciudad Real, Spain. JuanJose.Berzas@uclm.es
authors.forename: J J Berzas
authors.initials: JJ
authors.lastname: Nevado
authors.forename: G Castañeda
authors.initials: GC
authors.lastname: Peñalvo
authors.forename: R M Rodríguez
authors.initials: RM
authors.lastname: Dorado
mesh_terms: D000897:Anti-Ulcer Agents / Q000032:analysis / Q000302:isolation & purification; D019075:Electrophoresis, Capillary / Q000379:methods*; D006635:Histamine H2 Antagonists / Q000032:analysis* / Q000302:isolation & purification*; D015203:Reproducibility of Results
chemical_list: D000897:Anti-Ulcer Agents; D006635:Histamine H2 Antagonists