title: Correlation of oocyte morphometry parameters with woman's age.
abstract: OBJECTIVE
  Aim of this study was to evaluate morphometric parameters of metaphase II oocytes, including cytoplasm diameter (CD), zona pellucida thickness (ZPT) and width of the perivitelline space (PS), in relation with zona pellucida birefringence, spindle presence and age of the woman.
  
  
  METHODS
  Oocytes were classified into groups according to zona birefringence (low or high zona birefringence, LZB and HZB, respectively) and presence or absence of a visible spindle (SP and aSP, respectively).
  
  
  RESULTS
  HZB oocytes showed a thicker zona (17.7 ± 0.3 μm) than LZB oocytes (16.7 ± 0.3 μm, p < 0.01). Moreover, PS was narrower in HZB and SP oocytes than in LZB (p < 0,001) and aSP (p < 0,05) oocytes. Finally, we found that CD and ZPT linearly decrease with age of the woman (CD r = 0.028: p < 0.01; ZPT r = 0.050: p < 0.0001).
  
  
  CONCLUSIONS
  Our results evidence an association in human oocytes between zona pellucida and spindle birefringence and defined morphometric parameters and a decrease of oocyte size and ZPT as a function of women's age.
authors.affiliation: Genesis Centre for Reproductive Medicine, via Velletri 7, 00198, Rome, Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Valeri
authors.forename: Sebastiana
authors.initials: S
authors.lastname: Pappalardo
authors.forename: Massimo
authors.initials: M
authors.lastname: De Felici
authors.forename: Claudio
authors.initials: C
authors.lastname: Manna
mesh_terms: D000367:Age Factors; D001718:Birefringence; D002478:Cells, Cultured; D003593:Cytoplasm; D047108:Embryonic Development / Q000502:physiology; D005260:Female; D005640:Follicle Stimulating Hormone / Q000008:administration & dosage; D006801:Humans; D008677:Metaphase; D009865:Oocytes / Q000166:cytology* / Q000254:growth & development; D011247:Pregnancy; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods; D015044:Zona Pellucida
chemical_list: D005640:Follicle Stimulating Hormone