title: Ceiling culture-derived proliferative adipocytes retain high adipogenic potential suitable for use as a vehicle for gene transduction therapy.
abstract: Adipose tissue is expected to provide a source of proliferative cells for regenerative medicine and cell-transplantation therapies using gene transfer manipulation. We have recently identified ceiling culture-derived proliferative adipocytes (ccdPAs) from the mature adipocyte fraction as cells suitable as a therapeutic gene vehicle because of their stable proliferative capacity. In this study, we examined the capability of adipogenic differentiation of the ccdPAs compared with stromal vascular fraction (SVF)-derived progenitor cells (adipose-derived stem cells, ASCs) with regard to their multipotential ability to be converted to another lineage and therefore their potential to be used for regenerative medicine research. After in vitro passaging, the surface antigen profile and the basal levels of adipogenic marker genes of the ccdPAs were not obviously different from those of the ASCs. However, the ccdPAs showed increased lipid-droplet accumulation accompanied with higher adipogenic marker gene expression after stimulation of differentiation compared with the ASCs. The higher adipogenic potential of the ccdPAs than the ASCs from the SVF was maintained for 42 days in culture. Furthermore, the difference in the adipogenic response was enhanced after partial stimulation without indomethacin. These results indicate that the ccdPAs retain a high adipogenic potential even after in vitro passaging, thus suggesting the commitment of ccdPAs to stable mature adipocytes after autotransplantation, indicating that they may have potential for use in regenerative and gene-manipulated medicine.
authors.affiliation: Center for Advanced Medicine, Chiba University Hospital, Chiba University, Chiba, Japan.
authors.forename: Sakiyo
authors.initials: S
authors.lastname: Asada
authors.forename: Masayuki
authors.initials: M
authors.lastname: Kuroda
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Aoyagi
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Fukaya
authors.forename: Shigeaki
authors.initials: S
authors.lastname: Tanaka
authors.forename: Shunichi
authors.initials: S
authors.lastname: Konno
authors.forename: Masami
authors.initials: M
authors.lastname: Tanio
authors.forename: Masayuki
authors.initials: M
authors.lastname: Aso
authors.forename: Kaneshige
authors.initials: K
authors.lastname: Satoh
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Okamoto
authors.forename: Toshinori
authors.initials: T
authors.lastname: Nakayama
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Saito
authors.forename: Hideaki
authors.initials: H
authors.lastname: Bujo
mesh_terms: D017667:Adipocytes / Q000166:cytology* / Q000502:physiology*; D050156:Adipogenesis; D000954:Antigens, Surface; D049109:Cell Proliferation; D017690:Cell Transplantation; D002478:Cells, Cultured; D020869:Gene Expression Profiling; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D039902:Multipotent Stem Cells / Q000166:cytology / Q000502:physiology; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism / Q000502:physiology
chemical_list: D000954:Antigens, Surface