title: The effect of methylsulfonylmethane on the experimental colitis in the rat.
abstract: Methylsulfonylmethane (MSM), naturally occurring in green plants, fruits and vegetables, has been shown to exert anti-inflammatory and antioxidant effects. MSM is an organosulfur compound and a normal oxidative metabolite of dimethyl sulfoxide. This study was carried out to investigate the effect of MSM in a rat model of experimental colitis. Colitis was induced by intracolonic instillation of 1 ml of 5% of acetic acid. Rats were treated with MSM (400 mg/kg/day, orally) for 4 days. Animals were euthanized and distal colon evaluated histologically and biochemically. Tissue samples were used to measurement of malondialdehyde (MDA), myeloperoxidase (MPO), catalase (CAT), glutathione (GSH) and proinflammatory cytokine (TNF-α and IL-1β) levels. Results showed that MSM decreased macroscopic and microscopic colonic damage scores caused by administration of acetic acid. MSM treatment also significantly reduced colonic levels of MDA, MPO and IL-1β, while increased the levels of GSH and CAT compared with acetic acid-induced colitis group. It seems that MSM as a natural product may have a protective effect in an experimental ulcerative colitis.
authors.affiliation: Department of Pharmacology, School of Medicine, Ardabil University of Medical Sciences, P.O. Box 56197, Ardabil, Iran. k.amirshahrokhi@arums.ac.ir
authors.forename: K
authors.initials: K
authors.lastname: Amirshahrokhi
authors.forename: S
authors.initials: S
authors.lastname: Bohlooli
authors.forename: M M
authors.initials: MM
authors.lastname: Chinifroush
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D002374:Catalase / Q000378:metabolism; D003092:Colitis / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D004121:Dimethyl Sulfoxide / Q000627:therapeutic use*; D005978:Glutathione / Q000032:analysis; D053583:Interleukin-1beta / Q000032:analysis; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013450:Sulfones / Q000627:therapeutic use*; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
chemical_list: D000893:Anti-Inflammatory Agents; D053583:Interleukin-1beta; D013450:Sulfones; D014409:Tumor Necrosis Factor-alpha; D008315:Malondialdehyde; C025910:dimethyl sulfone; D002374:Catalase; D009195:Peroxidase; D005978:Glutathione; D004121:Dimethyl Sulfoxide