title: [Study of the correlation between 51 immune infertility patients of the Han nationality in Anhui and HLA-DQA1 gene and treatment of Mianbu III].
abstract: OBJECTIVE
  To explore the correlation between anti-sperm antibody-positive immune infertility patients and human leucocyte antigen (HLA) DQA1 gene, and to study the correlation between the treatment of Chinese medicine and pharmacy and HLA-DQA1 genotype.
  
  
  METHODS
  The polymerase chain reaction-sequence specific primers (PCR-SSP) technique was used in studying HLA-DQA1 genotypes of 51 anti-sperm antibody-positive immune infertility patients and 60 healthy subjects. Mianbu III (consisting of rehmannia root, white peony root, Fructus Comi, yam, barbary wolfberry fruit, moutan bark, and dwarf lilyturf root) was used in patients for three months.
  
  
  RESULTS
  The HLA-DQA1 *0401 allele in the immune infertility group was obviously higher than that in the healthy control group (chi2 = 29.869, P < 0.01). As for the therapeutic efficacy 22 cases of the 27 positive HLA-DQA1 * 0401 turned to negative with statistical difference (chi2 = 5.24, P = 0.022).
  
  
  CONCLUSIONS
  HLA-DQA1 *0401 allele might be predisposing gene of the anti-sperm antibody-positive immune infertility. The Chinese medicinal treatment was effective for the HLA-DQA1 *0401 allele patients.
authors.affiliation: Department of Histology, Anhui College of Traditional Chinese Medicine, Hefei.
authors.forename: Xin
authors.initials: X
authors.lastname: Bao
authors.forename: Wang-jiu
authors.initials: WJ
authors.lastname: Wang
authors.forename: Ning
authors.initials: N
authors.lastname: Dai
mesh_terms: D000328:Adult; D000483:Alleles; D044466:Asian People / Q000235:genetics; D016022:Case-Control Studies; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D059848:HLA-DQ alpha-Chains / Q000235:genetics*; D006801:Humans; D007246:Infertility / Q000188:drug therapy* / Q000235:genetics*; D008297:Male; D008517:Phytotherapy
chemical_list: D004365:Drugs, Chinese Herbal; D059848:HLA-DQ alpha-Chains; C069050:HLA-DQA1 antigen