title: [Study of the bone-like apatite's depositing induced by collagen I with its mechanism].
abstract: The collagen I was made with the dialysis method of enzymolysising the pig skin, and the static deposition in vitro of calcium phosphate was comparative studied by X-ray diffraction (XRD) and infrared spectroscopy (FTIR) under the condition of pH7. 4, Ca/P 1.67 and whether adding the collagen I into the system. Then the chemical composition of the sedimentary product and the diversification of the collagen I 's IR and Raman spectra (RS) before and after the mineralization were analyzed. The results showed that,under the physiological pH condition that there was not any collagen I, though Ca/P reached up to 1.67, the sedimentary product was CaHPO4 x 2H2O yet, however, after adding collagen I into the system, the bone-like apatite was deposited, which proved that collagen I indeed had the effects on the inducing of the bone-like apatite's mineralization in vitro; there was obviously mutual coordination action between collagen I and its mineralization product--bone-like apatite, which caused that amide peak I red-shifted, amide peak II and amide peak III decreased significantly or disappeared on the IR of collagen I, which maybe was the mechanism that how collagen I induced the depositing of the bone-like apatite.
authors.affiliation: College of Materials Science and Engineering, Sichuan University, Chengdu 610065, China.
authors.forename: Jianglei
authors.initials: J
authors.lastname: Tu
authors.forename: Fuqiang
authors.initials: F
authors.lastname: Guo
authors.forename: Chunchun
authors.initials: C
authors.lastname: Lu
authors.forename: Bogang
authors.initials: B
authors.lastname: Li
mesh_terms: D000818:Animals; D001031:Apatites / Q000378:metabolism*; D024042:Collagen Type I / Q000494:pharmacology*; D010012:Osteogenesis / Q000187:drug effects*; D012867:Skin / Q000737:chemistry*; D013552:Swine
chemical_list: D001031:Apatites; D024042:Collagen Type I