title: WST-8 analysis of cell viability during osteogenesis of human mesenchymal stem cells.
abstract: WST-8 is one of the newer generation formazan-based dyes, which release the converted product into the medium in a soluble form. This allows for a non-destructive determination of viability enabling the cells to be subject to further investigations. This is a major advantage in cases where cell phenotype is being investigated and data such as matrix synthesis is correlated to cell number. This chapter describes the use of WST-8 to normalise alkaline phosphatase activity.
authors.affiliation: AO Research Institute Davos, Davos Platz, Switzerland. martin.stoddart@aofoundation.org
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Stoddart
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D002470:Cell Survival; D002478:Cells, Cultured; D003584:Cytological Techniques / Q000379:methods*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000201:enzymology; D010012:Osteogenesis; D013778:Tetrazolium Salts / Q000378:metabolism*
chemical_list: C476329:2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H tetrazolium monosodium salt; D013778:Tetrazolium Salts; D000469:Alkaline Phosphatase