title: Loop domain organization of the p53 locus in normal and breast cancer cells correlates with the transcriptional status of the TP53 and the neighboring genes.
abstract: P53 is a tumor suppressor protein critical for genome integrity. Although its control at the protein level is well known, the transcriptional regulation of the TP53 gene is still unclear. We have analyzed the organization of the TP53 gene domain using DNA arrays in several breast cancer and control cell lines. We have found that in the control breast epithelial cell line, HB2, the TP53 gene is positioned within a relatively small DNA domain, encompassing 50 kb, delimited by two nuclear matrix attachment sites. Interestingly, this domain structure was found to be radically different in the studied breast cancer cell lines, MCF7, T47D, MDA-MB-231, and BT474, in which the domain size was increased and TP53 transcription was decreased. We propose a model in which the organization of the TP53 gene domain correlates with the transcriptional status of TP53 and neighboring genes.
authors.affiliation: Unité Mixte de Recherche 8126, Signalisation, Noyaux et Innovations en Cancérologie, Centre NaTional de la Recherche Scientifique, Institut de Cancérologie Gustave-Roussy, Université Paris-Sud 11, F-94805 Villejuif Cedex, France.
authors.forename: Andrea C S
authors.initials: AC
authors.lastname: Goes
authors.forename: David
authors.initials: D
authors.lastname: Cappellen
authors.forename: Gilson C
authors.initials: GC
authors.lastname: Santos
authors.forename: Iryna
authors.initials: I
authors.lastname: Pirozhkova
authors.forename: Marc
authors.initials: M
authors.lastname: Lipinski
authors.forename: Yegor
authors.initials: Y
authors.lastname: Vassetzky
authors.forename: Claudia V
authors.initials: CV
authors.lastname: de Moura-Gallo
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism; D005260:Female; D056426:Genetic Loci; D006367:HeLa Cells; D006801:Humans; D008075:Lipoproteins, HDL / Q000235:genetics / Q000378:metabolism; D008957:Models, Genetic; D015530:Nuclear Matrix / Q000235:genetics / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D018110:Receptors, Lipoprotein / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D016159:Tumor Suppressor Protein p53
chemical_list: D008075:Lipoproteins, HDL; D018110:Receptors, Lipoprotein; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; C031616:high density lipoprotein receptors