title: RASSF1A suppresses the activated K-Ras-induced oxidative DNA damage.
abstract: The mutant K-Ras elevates intracellular reactive oxygen species (ROS) levels and leads to oxidative DNA damage, resulting in malignant cell transformation. Ras association domain family 1 isoform A (RASSF1A) is known to play a role as a Ras effector. However, the suppressive effect of RASSF1A on K-RasV12-induced ROS increase and DNA damage has not been identified. Here, we show that RASSF1A blocks K-RasV12-triggered ROS production. RASSF1A expression also inhibits oxidative DNA damage and chromosomal damage. From the results obtained in this study, we suggest that RASSF1A regulates the cellular ROS levels enhanced by the Ras signaling pathway, and that it may function as a tumor suppressor by suppressing DNA damage caused by activated Ras.
authors.affiliation: Laboratory of Molecular Cell Biology, Graduate School of Medicine, Korea University College of Medicine, Korea University, Seoul 136-705, Republic of Korea.
authors.forename: Seon Ho
authors.initials: SH
authors.lastname: Park
authors.forename: Jung Jin
authors.initials: JJ
authors.lastname: Kim
authors.forename: Jin Sil
authors.initials: JS
authors.lastname: Chung
authors.forename: So Ra
authors.initials: SR
authors.lastname: Lee
authors.forename: Gi Young
authors.initials: GY
authors.lastname: Lee
authors.forename: Hyung Jung
authors.initials: HJ
authors.lastname: Kim
authors.forename: Young Do
authors.initials: YD
authors.lastname: Yoo
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004249:DNA Damage; D011905:Genes, ras; D006801:Humans; D051379:Mice; D041681:NIH 3T3 Cells; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors / Q000378:metabolism*; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: C411762:RASSF1 protein, human; D017382:Reactive Oxygen Species; D025521:Tumor Suppressor Proteins