title: Isolation and characterization of endocellulase-free multienzyme complex from newly isolated Thermoanaerobacterium thermosaccharolyticum strain NOI-1.
abstract: An endocellulase-free multienzyme complex was produced by a thermophilic anaerobic bacterium, Thermoanaerobacterium thermosaccharolyticum strain NOI-1, when grown on xylan. The temperature and pH optima for growth were 60 degrees C and 6.0, respectively. The bacterial cells were found to adhere to insoluble xylan and Avicel. A scanning electron microscopy analysis showed the adhesion of xylan to the cells. An endocellulase-free multienzyme complex was isolated from the crude enzyme of strain NOI-1 by affinity purification on cellulose and Sephacryl S-300 gel filtration. The molecular mass of the multienzyme complex was estimated to be about 1,200 kDa. The multienzyme complex showed one protein on native PAGE, one xylanase on a native zymogram, 21 proteins on SDS-PAGE, and 5 xylanases on a SDS zymogram. The multienzyme complex consisted of xylanase, beta-xylosidase, alpha-L-arabinofuranosidase, beta-glucosidase, and cellobiohydrolase. The multienzyme complex was effective in hydrolyzing xylan and corn hulls. This is the first report of an endocellulase-free multienzyme complex produced by a thermophilic anaerobic bacterium, T. thermosaccharolyticum strain NOI-1.
authors.affiliation: School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Bangkuntien, Bangkok, Thailand.
authors.forename: Suphavadee
authors.initials: S
authors.lastname: Chimtong
authors.forename: Chakrit
authors.initials: C
authors.lastname: Tachaapaikoon
authors.forename: Patthra
authors.initials: P
authors.lastname: Pason
authors.forename: Khin Lay
authors.initials: KL
authors.lastname: Kyu
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kosugi
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Mori
authors.forename: Khanok
authors.initials: K
authors.lastname: Ratanakhanokchai
mesh_terms: D001422:Bacterial Adhesion; D044602:Cellulases / Q000302:isolation & purification; D002482:Cellulose / Q000378:metabolism; D002846:Chromatography, Affinity / Q000379:methods; D002850:Chromatography, Gel / Q000379:methods; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004586:Electrophoresis / Q000379:methods; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009097:Multienzyme Complexes / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D010802:Phylogeny; D011485:Protein Binding; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D046950:Thermoanaerobacterium / Q000201:enzymology* / Q000254:growth & development / Q000502:physiology; D014990:Xylans / Q000378:metabolism*; D003313:Zea mays / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D009097:Multienzyme Complexes; D012336:RNA, Ribosomal, 16S; D014990:Xylans; D002482:Cellulose; D044602:Cellulases