title: Enrichment of a novel marine ammonia-oxidizing archaeon obtained from sand of an eelgrass zone.
abstract: Ammonia-oxidizing archaea (AOA) are generally cultivated at ammonium concentrations of less than 2 mM. The physiology and abundance in the environment of AOA suggest an important role in the nitrogen cycle. We report here a novel marine ammonia-oxidizing crenarchaeote, strain NM25 belonged to 'Candidatus Nitrosopumilus', that was enriched from coastal sand of an eelgrass zone and grew in a medium containing 15 mM ammonium at 30°C. A phylogenetic analysis based on the 16S rRNA gene revealed this crenarchaeote was related to the ammonia-oxidizing archaeon 'Candidatus Nitrosopumilus maritimus' strain SCM1, with 98.5% identity. The ammonia monooxygenase subunit A (amoA) gene of strain NM25 was less closely related to that of known cultivable AOA (>95%) and environmental clones (>97%). This finding suggests the existence of AOA adapted to high ammonium-containing environments.
authors.affiliation: Graduate School of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252–0880, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Matsutani
authors.forename: Tatsunori
authors.initials: T
authors.lastname: Nakagawa
authors.forename: Kyoko
authors.initials: K
authors.lastname: Nakamura
authors.forename: Reiji
authors.initials: R
authors.lastname: Takahashi
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Yoshihara
authors.forename: Tatsuaki
authors.initials: T
authors.lastname: Tokuyama
mesh_terms: D000641:Ammonia / Q000378:metabolism*; D001105:Archaea / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D019843:Archaeal Proteins / Q000235:genetics / Q000378:metabolism; D019015:Geologic Sediments / Q000382:microbiology*; D031321:Hydrocharitaceae / Q000382:microbiology*; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D010802:Phylogeny; D012623:Seawater / Q000382:microbiology*; D012822:Silicon Dioxide / Q000032:analysis
chemical_list: D019843:Archaeal Proteins; D012822:Silicon Dioxide; D000641:Ammonia; D010088:Oxidoreductases; C045582:ammonia monooxygenase