title: Responses of sugar maple and hemlock seedlings to elevated carbon dioxide under altered above- and belowground nitrogen sources.
abstract: Various human-induced changes to the atmosphere have caused carbon dioxide (CO₂), nitrogen dioxide (NO₂) and nitrate deposition (NO₃⁻) to increase in many regions of the world. The goal of this study was to examine the simultaneous influence of these three factors on tree seedlings. We used open-top chambers to fumigate sugar maple (Acer saccharum) and eastern hemlock (Tsuga canadensis) with ambient or elevated CO₂ and NO₂ (elevated concentrations were 760 ppm and 40 ppb, respectively). In addition, we applied an artificial wet deposition of 30 kg ha⁻¹ year⁻¹ NO₃⁻ to half of the open-top chambers. After two growing seasons, hemlocks showed a stimulation of growth under elevated CO₂, but the addition of elevated NO₂ or NO₃⁻ eliminated this effect. In contrast, sugar maple seedlings showed no growth enhancement under elevated CO₂ alone and decreased growth in the presence of NO₂ or NO₃⁻, and the combined treatments of elevated CO₂ with increased NO₂ or NO₃⁻ were similar to control plants. Elevated CO₂ induced changes in the leaf characteristics of both species, including decreased specific leaf area, decreased %N and increased C:N. The effects of elevated CO₂, NO₂ and NO₃⁻ on growth were not additive and treatments that singly had no effect often modified the effects of other treatments. The growth of both maple and hemlock seedlings under the full combination of treatments (CO₂ + NO₂ + NO₃⁻) was similar to that of seedlings grown under control conditions, suggesting that models predicting increased seedling growth under future atmospheric conditions may be overestimating the growth and carbon storage potential of young trees.
authors.affiliation: Ecology and Evolutionary Biology Department, Cornell University, Corson Hall Rm E150, Ithaca, NY 14853, USA.
authors.forename: Allyson S D
authors.initials: AS
authors.lastname: Eller
authors.forename: Krista L
authors.initials: KL
authors.lastname: McGuire
authors.forename: Jed P
authors.initials: JP
authors.lastname: Sparks
mesh_terms: D031002:Acer / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D001272:Atmosphere; D018533:Biomass; D002245:Carbon Dioxide / Q000494:pharmacology*; D002980:Climate; D006801:Humans; D009566:Nitrates / Q000494:pharmacology*; D009585:Nitrogen Dioxide / Q000494:pharmacology*; D010788:Photosynthesis; D018515:Plant Leaves / Q000254:growth & development; D018517:Plant Roots / Q000254:growth & development; D018520:Plant Shoots / Q000254:growth & development; D036226:Seedlings / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D013997:Time Factors; D028225:Tsuga / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D014481:United States
chemical_list: D009566:Nitrates; D002245:Carbon Dioxide; D009585:Nitrogen Dioxide