title: [Fragmentation of DNA lymphocytes the human in dynamics of development apoptosis, induced influence of UV-radiation and reactive oxygen species].
abstract: It is established that UV-light (240-390 nm) in doses of 151, 1510 and 3020 J/m2 and reactive oxygen species and singlet oxygen induce DNA fragmentation lymphocytes cells of the human 20 h after influence. Using a method of DNA-comets it is revealed that DNA damages (single strand breaks) are found out right after UV-irradiations of lymphocytes in doses of 1510 and 3020 J/m2 and additions hydrogen peroxide in concentration of 10-6 mol/l (a comet of type C1) and reach a maximum through 6 h after influence on of cells UV-light and ROS (comets of types C2 and C3). Assumption about the leading part of a p53-dependent way in realization apoptosis human lymphocytes in the conditions of influence of UV-light and reactive oxygen species is put forward.
authors.forename: V G
authors.initials: VG
authors.lastname: Artiukhov
authors.forename: M S
authors.initials: MS
authors.lastname: Trubitsyna
authors.forename: M A
authors.initials: MA
authors.lastname: Nakvasina
authors.forename: E V
authors.initials: EV
authors.lastname: Solov'eva
mesh_terms: D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis / Q000737:chemistry; D053904:DNA Breaks, Single-Stranded / Q000187:drug effects* / Q000528:radiation effects*; D053938:DNA Fragmentation / Q000187:drug effects / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D004587:Electrophoresis, Agar Gel; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D017665:Hydroxyl Radical / Q000378:metabolism; D018655:Lymphocyte Count; D008214:Lymphocytes / Q000737:chemistry / Q000166:cytology / Q000187:drug effects* / Q000528:radiation effects*; D026082:Singlet Oxygen / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: D016159:Tumor Suppressor Protein p53; D026082:Singlet Oxygen; D017665:Hydroxyl Radical; D004247:DNA; D006861:Hydrogen Peroxide