title: Aldose reductase inhibitory, anti-cataract and antioxidant potential of selected medicinal plants from the Marathwada region, India.
abstract: The water, ethanol and chloroform extracts of selected plants such as Adhatoda vasica (L.) (Acanthaceae), Caesalpinia bonduc (L.), Cassia fistula (L.) (Caesalpiniaceae) and Biophytum sensitivum (L.) (Oxalidaceae) were evaluated for rat lens aldose reductase inhibitory (RLAR) potential, anti-cataract and antioxidant activities. All the samples inhibited the aldose reductase considerably and exhibited anti-cataract activity, while C. fistula (IC(50), 0.154 mg mL(-1)) showed significant RLAR inhibitory activity as compared to the other tested samples, and was further found to be more effective in maintaining sugar-induced lens opacity in the rat lens model. The antioxidant potential of plant extracts was determined using DPPH (2,2-diphenyl-1-picryl hydrazine), hydroxyl (OH), nitric oxide (NO) and hydrogen peroxide (H(2)O(2)) scavenging activities, along with determination of reducing power, ferrous ion chelating ability and inhibition of polyphenol oxidase (PPO). The extracts of the tested plant showed significant free radical scavenging activities and inhibited the activity of enzyme PPO, a model oxidising enzyme. The plant samples were found to possess considerable amounts of vitamin C, total polyphenols and flavonoids.
authors.affiliation: School of Life Sciences, Swami Ramanand Teerth Marathwada University, Nanded 431606 (MS), India. rngacche@rediffmail.com
authors.forename: R N
authors.initials: RN
authors.lastname: Gacche
authors.forename: N A
authors.initials: NA
authors.lastname: Dhole
mesh_terms: D000449:Aldehyde Reductase / Q000037:antagonists & inhibitors*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001713:Biphenyl Compounds; D002386:Cataract / Q000188:drug therapy; D004156:Catechol Oxidase / Q000037:antagonists & inhibitors; D006861:Hydrogen Peroxide; D007194:India; D020128:Inhibitory Concentration 50; D007502:Iron Chelating Agents; D010851:Picrates; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry*; D051381:Rats
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D007502:Iron Chelating Agents; D010851:Picrates; D010936:Plant Extracts; D006861:Hydrogen Peroxide; C004931:1,1-diphenyl-2-picrylhydrazyl; D000449:Aldehyde Reductase; D004156:Catechol Oxidase