title: Sheep ovarian tissue vitrification by two different dehydration protocols and needle immersing methods.
abstract: The present research investigated the effects of two vitrification methods on sheep ovarian tissue. The base medium (BM) of the vitrification solutions contains 60% HEPES tissue culture medium (HTCM), 15% ethylene glycol (EG) and 15% dimethyl sulfoxide (DMSO). Ovarian cortex pieces were dehydrated by two different regimens, the 2-step which consisted of 50% BM and a 90% solution of 0.25 mol/L sucrose in BM for 10 minutes each at 4 degree C and the 4-step method which utilized: a) 25% BM, b) 50% BM, c) 75% BM and d) a 90% solution of 0.25 mol/L sucrose in BM for 5 minutes each at 4 degree C. After warming, the proportion of intact antral follicles in the control (non-vitrified) and 2-step vitrification groups was significantly higher than in the 4-step vitrification group. The number of apoptotic follicles in the ovarian pieces was significantly different between the control and 4-step vitrification groups. These results indicated that sheep ovarian tissue vitrification by the 2-step method was simpler and more effective than the 4-step method.
authors.affiliation: Department of Embryology, Infertility and IVF Unit, Royan Institute, Tehran, Iran.
authors.forename: Rouhollah
authors.initials: R
authors.lastname: Fathi
authors.forename: Mojtaba Rezazadeh
authors.initials: MR
authors.lastname: Valojerdi
authors.forename: Hussein
authors.initials: H
authors.lastname: Eimani
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Hasani
authors.forename: Poopak Eftekhari
authors.initials: PE
authors.lastname: Yazdi
authors.forename: Zahra
authors.initials: Z
authors.lastname: Ajdari
authors.forename: Leila Sadat
authors.initials: LS
authors.lastname: Tahaei
mesh_terms: D000818:Animals; D003080:Cold Temperature; D015925:Cryopreservation; D003451:Cryoprotective Agents / Q000494:pharmacology; D003470:Culture Media; D003890:Desiccation / Q000379:methods*; D005260:Female; D007101:Immersion; D006080:Ovarian Follicle / Q000187:drug effects / Q000648:ultrastructure*; D012756:Sheep; D014021:Tissue Preservation; D058989:Vitrification
chemical_list: D003451:Cryoprotective Agents; D003470:Culture Media