title: Detection of fecal shedding of rotavirus vaccine in infants following their first dose of pentavalent rotavirus vaccine.
abstract: Studies on rotavirus vaccine shedding and its potential transmission within households including immunocompromised individuals are needed to better define the potential risks and benefits of vaccination. We examined fecal shedding of pentavalent rotavirus vaccine (RV5) for 9 days following the first dose of vaccine in infants between 6 and 12 weeks of age. Rotavirus antigen was detected by enzyme immunoassay (EIA), and vaccine-type rotavirus was identified by nucleotide sequencing based on genetic relatedness to the RV5 VP6 gene. Stool from 22 (21.4%) of 103 children contained rotavirus antigen-positive specimens on ≥ 1 post-vaccination days. Rotavirus antigen was detected as early as post-vaccination day 3 and as late as day 9, with peak numbers of shedding on post-vaccination days 6 through 8. Vaccine-type rotavirus was detected in all 50 antigen-positive specimens and 8 of 8 antigen-negative specimens. Nine (75%) of 12 EIA-positive and 1 EIA-negative samples tested culture-positive for vaccine-type rotavirus. Fecal shedding of rotavirus vaccine virus after the first dose of RV5 occurred over a wide range of post-vaccination days not previously studied. These findings will help better define the potential for horizontal transmission of vaccine virus among immunocompromised household contacts of vaccinated infants for future studies.
authors.affiliation: Department of Pediatrics, Columbia University, New York, NY 10032, USA.
authors.forename: Catherine
authors.initials: C
authors.lastname: Yen
authors.forename: Kathleen
authors.initials: K
authors.lastname: Jakob
authors.forename: Mathew D
authors.initials: MD
authors.lastname: Esona
authors.forename: Ximara
authors.initials: X
authors.lastname: Peckham
authors.forename: John
authors.initials: J
authors.lastname: Rausch
authors.forename: Jennifer J
authors.initials: JJ
authors.lastname: Hull
authors.forename: Susan
authors.initials: S
authors.lastname: Whittier
authors.forename: Jon R
authors.initials: JR
authors.lastname: Gentsch
authors.forename: Philip
authors.initials: P
authors.lastname: LaRussa
mesh_terms: D000956:Antigens, Viral / Q000032:analysis / Q000235:genetics; D036022:Capsid Proteins / Q000235:genetics; D005243:Feces / Q000737:chemistry / Q000821:virology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007223:Infant; D012367:RNA, Viral / Q000235:genetics; D022243:Rotavirus Vaccines / Q000008:administration & dosage*; D017422:Sequence Analysis, DNA; D013997:Time Factors; D014613:Vaccines, Attenuated / Q000008:administration & dosage; D017201:Virus Shedding
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; D012367:RNA, Viral; C492535:RotaTeq; D022243:Rotavirus Vaccines; C061332:VP6 protein, Rotavirus; D014613:Vaccines, Attenuated