title: Application of flow cytometry in pediatric hematology-oncology.
abstract: Applications of flow cytometry in pediatric cancers have expanded substantially in recent years. In acute leukemias, the commonest childhood cancer, flow cytometry can now define complex antigenic profiles that are associated with specific cytogenetic/molecular defects and can also directly identify BCR-ABL fusion protein. Flow cytometry based scoring system has been described for diagnosis of myelodysplastic syndromes. In solid tumors, flow cytometry was previously used mainly to determine DNA content for prognosis; however, recent studies in children with neuroblastoma and Ewing sarcoma have identified its diagnostic utility. In this review, we will discuss the current and future applications of flow cytometry in pediatric hematology-oncology.
authors.affiliation: Department of Hematology, Postgraduate Institute of Medical Education and Research, India. varmaneelam@yahoo.com
authors.forename: Neelam
authors.initials: N
authors.lastname: Varma
authors.forename: Shano
authors.initials: S
authors.lastname: Naseem
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D004273:DNA, Neoplasm / Q000097:blood; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007223:Infant; D008297:Male; D009369:Neoplasms / Q000097:blood* / Q000175:diagnosis*; D011379:Prognosis
chemical_list: D004273:DNA, Neoplasm