title: Pyridine inhibitor binding to the 4Fe-4S protein A. aeolicus IspH (LytB): a HYSCORE Investigation.
abstract: IspH is a 4Fe-4S protein that carries out an essential reduction step in isoprenoid biosynthesis. Using hyperfine sublevel correlation (HYSCORE) spectroscopy, we show that pyridine inhibitors of IspH directly bind to the unique fourth Fe in the 4Fe-4S cluster, opening up new routes to inhibitor design, of interest in the context of both anti-bacterial as well as anti-malarial drug discovery.
authors.affiliation: Center for Biophysics and Computational Biology, 607 South Mathews Avenue, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.
authors.forename: Weixue
authors.initials: W
authors.lastname: Wang
authors.forename: Jikun
authors.initials: J
authors.lastname: Li
authors.forename: Ke
authors.initials: K
authors.lastname: Wang
authors.forename: Tatyana I
authors.initials: TI
authors.lastname: Smirnova
authors.forename: Eric
authors.initials: E
authors.lastname: Oldfield
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D015195:Drug Design; D007506:Iron-Sulfur Proteins / Q000378:metabolism*; D008958:Models, Molecular; D011485:Protein Binding; D011725:Pyridines / Q000737:chemistry* / Q000494:pharmacology*; D013729:Terpenes / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D007506:Iron-Sulfur Proteins; D011725:Pyridines; D013729:Terpenes; C023666:pyridine