title: [The RHD zygosity in Dong ethnic population in the southeast area of Guizhou province].
abstract: OBJECTIVE
  To investigate the RHD zygosity distribution and the genetic characteristics of RHD gene in the Dong ethnic population in southeast area of Guizhou province.
  
  
  METHODS
  Based on the characteristics of Chinese RHD and the RHD specific deletion, two pairs of primers specific for hybrid Rhesus box and exon 1 of RHD respectively, were designed, combined with a pair of internal control primers. Polymerase chain reaction was performed to genotype the samples.
  
  
  RESULTS
  In the 292 RhD positive samples, 58 (19.86%) were RHD+/RHD- heterozygotes, and the others (80.14%) were RHD+/RHD+ homozygotes. In the 9 D negative samples, 5 were RHD+/RHD- heterozygotes (2 weak D, 3 Del), 3 were RHD+/RHD+ homozygotes (1weak D, 2 Del), and 1 was RHD-/RHD- homozygote.
  
  
  CONCLUSIONS
  RHD+/RHD- heterozygosity is higher (19.86%) in D positive individuals of Dong ethnic group in Guizhou province than that in other areas; RHD gene heterozygosity is also high in the D negative individuals in this ethnic group.
authors.affiliation: Department of Clinical Laboratory Medicine, Guiyang Medical College, Guiyang, Guizhou, 550004 P .R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: HUANG
authors.forename: Shuo
authors.initials: S
authors.lastname: CONG
authors.forename: Xiao-jing
authors.initials: XJ
authors.lastname: ZENG
authors.forename: Fang
authors.initials: F
authors.lastname: YANG
authors.forename: Lin-sheng
authors.initials: LS
authors.lastname: XIAO
mesh_terms: D002681:China; D005006:Ethnicity / Q000235:genetics*; D005091:Exons; D017353:Gene Deletion; D005838:Genotype; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D012204:Rh-Hr Blood-Group System / Q000235:genetics*
chemical_list: D012204:Rh-Hr Blood-Group System