title: Developmental expression of ACRV1 in humans and mice.
abstract: To identify the developmental expression of the ACRV1 gene in humans and mice, testes cDNA samples were collected at different post-natal days (days 4, 9, 18, 35, 54, and 6 months) from Balb/c mice and were hybridised to the mouse whole genome 430 2.0 Array (Affymetrix Inc.) chip. The characteristics of ACRV1 were analysed using various cellular and molecular biotechnologies. The results showed that the expression of mouse ACRV1 was not detected in mouse testes on days 4, 9, and 18 but was present on days 35, 54, and 6 months. Using RT-PCR analysis of mouse ACRV1, we determined that mouse ACRV1 was expressed specifically in the mouse testis, and its expression began at days 35. Western blot analysis demonstrated that human ACRV1 was primarily expressed in human testes, and immunofluorescent and immunohistochemistry staining showed that human ACRV1 protein was predominantly located in round and elongated spermatids in human testes, indicating that ACRV1 may play an important role in mammalian spermatogenesis and may be a target of a contraceptive vaccine.
authors.affiliation: Guangdong Key Lab of Male Reproductive Medicine and Genetics, Peking University Shenzhen Hospital, Shenzhen, Guangdong, China.
authors.forename: A
authors.initials: A
authors.lastname: Tang
authors.forename: Q
authors.initials: Q
authors.lastname: Yan
authors.forename: L
authors.initials: L
authors.lastname: Sun
authors.forename: R
authors.initials: R
authors.lastname: Diao
authors.forename: Z
authors.initials: Z
authors.lastname: Yu
authors.forename: Z
authors.initials: Z
authors.lastname: Zhang
authors.forename: Y
authors.initials: Y
authors.lastname: Gui
authors.forename: Z
authors.initials: Z
authors.lastname: Cai
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D019295:Computational Biology; D017931:DNA Primers / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013045:Species Specificity; D013737:Testis / Q000254:growth & development* / Q000378:metabolism*
chemical_list: C512729:ACRV1 protein, human; C080034:Acrv1 protein, mouse; D017931:DNA Primers; D008565:Membrane Proteins; D012333:RNA, Messenger