title: Study of cell-matrix adhesion dynamics using surface plasmon resonance imaging ellipsometry.
abstract: The interaction of cells with extracellular matrix, termed cell-matrix adhesions, importantly governs multiple cellular phenomena. Knowledge of the functional dynamics of cell-matrix adhesion could provide critical clues for understanding biological phenomena. We developed surface plasmon resonance imaging ellipsometry (SPRIE) to provide high contrast images of the cell-matrix interface in unlabeled living cells. To improve the contrast and sensitivity, the null-type imaging ellipsometry technique was integrated with an attenuated total reflection coupler. We verified that the imaged area of SPRIE was indeed a cell-matrix adhesion area by confocal microscopy imaging. Using SPRIE, we demonstrated that three different cell types exhibit distinct features of adhesion. SPRIE was applied to diverse biological systems, including during cell division, cell migration, and cell-cell communication. We imaged the cell-matrix anchorage of mitotic cells, providing the first label-free imaging of this interaction to our knowledge. We found that cell-cell communication can alter cell-matrix adhesion, possibly providing direct experimental evidence for cell-cell communication-mediated changes in cell adhesion. We also investigated shear-stress-induced adhesion dynamics in real time. Based on these data, we expect that SPRIE will be a useful methodology for studying the role of cell-matrix adhesion in important biological phenomena.
authors.affiliation: Center for Nano-Bio Convergence Research, Korea Research Institute of Standards and Science, Daejeon, Republic of Korea.
authors.forename: Se-Hwa
authors.initials: SH
authors.lastname: Kim
authors.forename: Won
authors.initials: W
authors.lastname: Chegal
authors.forename: Junsang
authors.initials: J
authors.lastname: Doh
authors.forename: Hyun Mo
authors.initials: HM
authors.lastname: Cho
authors.forename: Dae Won
authors.initials: DW
authors.lastname: Moon
mesh_terms: D002448:Cell Adhesion; D002450:Cell Communication; D002455:Cell Division; D021982:Cell-Matrix Junctions / Q000378:metabolism*; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D018613:Microscopy, Confocal; D008938:Mitosis; D057054:Molecular Imaging / Q000379:methods*; D015203:Reproducibility of Results; D013194:Staining and Labeling; D013314:Stress, Mechanical; D020349:Surface Plasmon Resonance / Q000379:methods*; D013997:Time Factors