title: The effects of supplementary bacterial phytase on dietary true metabolisable energy, nutrient digestibility and endogenous losses in precision fed turkeys.
abstract: 1. A total of 40 female BUT9 turkeys were used in a precision-feeding assay to investigate the effect of dietary phytase on true metabolisable energy corrected for N retention (TME(N)), coefficients of true dry matter (TDMD), mineral, amino acid and nitrogen (TND) digestibilities and the excretion of endogenous mucin, measured as sialic acid (SA). 2. Five treatments were used in this study: control (C), C + 250 phytase units (FTU) per kg feed, C + 500 FTU, C + 2500 FTU, and glucose only for endogenous losses estimation. Diets were formulated to be nutritionally adequate with the exception that the P content was relatively low (3·6 g/kg non-phytate P). 3. Inclusion of phytase increased TND in a quadratic manner with the optimum being achieved at approximately 500 FTU, at which TND was 37 % greater than in the control. The concentration of SA in the excreta decreased linearly with increased phytase supplementation. Dietary TME(N), TDMD and true mineral digestibility coefficients were not significantly affected by phytase supplementation. 4. Phytase inclusion increased digestibility coefficients for indispensable, dispensable and total amino acids in a linear manner. The scale of the response to phytase was greatest with threonine and least with lysine digestibility, suggesting a specific mechanism of action that benefits gut health. 5. The strong negative relationship between secretion of SA and threonine digestibility suggests that a large part of the threonine benefit may be from reduced mucin synthesis. This supports the hypothesis that dietary phytase may play a role in improving the health status of the intestine and, as a result, reduces the maintenance energy requirements of turkeys.
authors.affiliation: ASRC, SAC, West Mains Road, Edinburgh, EH9 3JG, UK. vasil.pirgozliev@sac.ac.uk
authors.forename: V
authors.initials: V
authors.lastname: Pirgozliev
authors.forename: M R
authors.initials: MR
authors.lastname: Bedford
authors.forename: T
authors.initials: T
authors.lastname: Acamovic
authors.forename: M
authors.initials: M
authors.lastname: Allimehr
mesh_terms: D010832:6-Phytase / Q000378:metabolism / Q000494:pharmacology*; D000821:Animal Feed; D000818:Animals; D019587:Dietary Supplements; D004063:Digestion / Q000187:drug effects*; D004734:Energy Metabolism / Q000187:drug effects*; D004926:Escherichia coli / Q000201:enzymology; D029968:Escherichia coli Proteins / Q000494:pharmacology*; D005260:Female; D014422:Turkeys / Q000502:physiology*
chemical_list: D029968:Escherichia coli Proteins; D010832:6-Phytase