title: Three Mx genes with differential response to VNNV infection have been identified in Gilthead seabream (Sparus aurata).
abstract: Type I interferons are secreted by infected cells and promote an antiviral state in neighbouring cells by the induction of numerous genes, some of which present antiviral activity, as the Mx proteins. In this study, three different Mx cDNAs (Mx1, Mx2 and Mx3) from gilthead seabream (Sparus aurata), the most important fish species in Southern European aquaculture, have been cloned and characterized. A Southern blot assay revealed the existence of three Mx loci, thus the three Mx isoforms correspond to three different genes that seem to have a common origin. The genomic sequences of Mx1, Mx2 and Mx3 have been completely obtained, and consist on 11 introns and 12 exons in a full length of 5971 bp, 7391 bp and 6938 bp, respectively. As a first approach to the functional meaning of these three genes, their response to the viral nervous necrosis virus (VNNV) infection was tested. Important differences in terms of tissue, time course and level of induction were found between them, thus suggesting a differential functional role for each isoform, which can represent a key point in the natural resistance of this fish species, that has been repeatedly reported as an asymptomatic carrier of VNNV.
authors.affiliation: Department of Genetics, Faculty of Sciences, University of Málaga, 29071 Málaga, Spain.
authors.forename: M A
authors.initials: MA
authors.lastname: Fernández-Trujillo
authors.forename: P
authors.initials: P
authors.lastname: Novel
authors.forename: M
authors.initials: M
authors.lastname: Manchado
authors.forename: M P
authors.initials: MP
authors.lastname: Sepulcre
authors.forename: V
authors.initials: V
authors.lastname: Mulero
authors.forename: J J
authors.initials: JJ
authors.lastname: Borrego
authors.forename: M C
authors.initials: MC
authors.lastname: Alvarez
authors.forename: J
authors.initials: J
authors.lastname: Béjar
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015139:Blotting, Southern; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005091:Exons / Q000235:genetics; D005393:Fish Diseases / Q000235:genetics / Q000276:immunology / Q000821:virology*; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D016678:Genome / Q000235:genetics; D007438:Introns / Q000235:genetics; D008969:Molecular Sequence Data; D064547:Myxovirus Resistance Proteins; D030381:Nodaviridae / Q000276:immunology*; D012327:RNA Virus Infections / Q000235:genetics / Q000276:immunology / Q000821:virology*; D021541:Sea Bream / Q000235:genetics / Q000276:immunology* / Q000821:virology*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013997:Time Factors; D014158:Transcription, Genetic
chemical_list: D018076:DNA, Complementary; D029941:Fish Proteins; D064547:Myxovirus Resistance Proteins; D019204:GTP-Binding Proteins