title: Interleukin-1β stimulates stromal-derived factor-1α expression in human subacromial bursa.
abstract: Chemokines produced by synoviocytes of the subacromial bursa are up-regulated in subacromial bursitis and rotator cuff disease. We hypothesized that SDF-1α production in bursal synoviocytes may be induced by local cytokines such as interleukin IL-1β and IL-6. Subacromial bursa specimens were obtained from patients undergoing shoulder surgery. Bursal specimens were stained with anti-human antibodies to IL-1, IL-6, and SDF-1α by immunohistochemistry and compared to normal and rheumatoid controls. Bursal cells were also isolated from specimens and cultured. Early passaged cells were then treated with cytokines (IL-1β and IL-6) and SDF-1α expression was measured by ELISA and RT-PCR. SDF-1α, IL-1β, and IL-6 were expressed at high levels in bursitis specimens from human subacromial bursa compared to normal controls. In cultured bursal synoviocytes, there was a dose-dependent increase in SDF-1α production in the supernatants of cells treated with IL-1β. SDF-1α mRNA expression was also increased in bursal cells treated with IL-1β. IL-6 caused a minimal but not statistically significant increase in SDF-1α expression. SDF-1α, IL-1β, and IL-6 are expressed in the inflamed human subacromial bursal tissues in patients with subacromial bursitis. In cultured bursal synoviocytes, SDF-1α gene expression and protein production are stimulated by IL-1β. IL-1β produced by bursal syvoviocytes and inflammatory cells in the human subacromial bursa is an important signal in the inflammatory response that occurs in subacromial bursitis and rotator cuff disease.
authors.affiliation: Rhode Island Shoulder and Elbow Service, Brown Alpert Medical School, 2 Dudley Street, Suite 200, Providence, Rhode Island 02905, USA. tblaine@universityorthopedics.com
authors.forename: Theodore A
authors.initials: TA
authors.lastname: Blaine
authors.forename: Mindy A
authors.initials: MA
authors.lastname: Cote
authors.forename: Al
authors.initials: A
authors.lastname: Proto
authors.forename: Mary
authors.initials: M
authors.lastname: Mulcahey
authors.forename: Francis Y
authors.initials: FY
authors.lastname: Lee
authors.forename: Louis U
authors.initials: LU
authors.lastname: Bigliani
mesh_terms: D001706:Biopsy; D002061:Bursa, Synovial / Q000187:drug effects / Q000276:immunology* / Q000473:pathology; D002062:Bursitis / Q000276:immunology* / Q000473:pathology / Q000503:physiopathology; D002478:Cells, Cultured; D054377:Chemokine CXCL12 / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D015870:Gene Expression / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry; D053583:Interleukin-1beta / Q000276:immunology* / Q000494:pharmacology; D015850:Interleukin-6 / Q000276:immunology / Q000494:pharmacology; D017006:Rotator Cuff / Q000276:immunology* / Q000473:pathology; D019534:Shoulder Impingement Syndrome / Q000276:immunology* / Q000473:pathology / Q000503:physiopathology; D013583:Synovial Membrane / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D015854:Up-Regulation / Q000187:drug effects / Q000276:immunology
chemical_list: D054377:Chemokine CXCL12; C508600:IL6 protein, human; D053583:Interleukin-1beta; D015850:Interleukin-6