title: Optimisation of biotransformation conditions for production of 2-phenylethanol by a Saccharomyces cerevisiae CWY132 mutant.
abstract: A mutant Saccharomyces cerevisiae CWY132 was isolated, producing 1.393 g L(-1) 2-phenylethanol (2-PE) in a batch process containing 5 g L(-1) L-phenylalanine (L-Phe), which is equivalent to an increase of 38.3% compared to the initial strain. In this study, biotransformation conditions of this strain were studied. We found glucose, KH2PO4, (NH4)2SO4, and amounts of inoculum cells had significant effects on the biotransformation process; in particular, the existence of (NH(4))(2)SO(4) in the medium strongly inhibited the yield of 2-PE, while an increase in the amount of inoculum had a positive correlation with the yield of 2-PE. The optimum condition for production of 2-PE was obtained using the following uniform design: glucose 34.16 g L(-1), yeast nitrogen base 0.17 g L(-1), MgSO4 0.5 g L(-1), KH2PO4 14.89 g L(-1), (NH4)2SO4 0 g L(-1), L-Phe 5 g L(-1), and an inoculum amount of 1.6 × 10(7) cells/mL. With the optimised conditions, the yield of 2-PE was further increased to 3.52 g L(-1) (an increase of 249.5%), which corresponds to a molar conversion rate of 95.19%.
authors.affiliation: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, PR China.
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Cui
authors.forename: Xiao
authors.initials: X
authors.lastname: Yang
authors.forename: Qingjia
authors.initials: Q
authors.lastname: Shen
authors.forename: Kun
authors.initials: K
authors.lastname: Wang
authors.forename: Tingheng
authors.initials: T
authors.lastname: Zhu
mesh_terms: D000645:Ammonium Sulfate / Q000737:chemistry; D001711:Biotransformation; D003470:Culture Media / Q000737:chemistry*; D005947:Glucose / Q000737:chemistry; D007218:Industrial Microbiology / Q000379:methods*; D010649:Phenylalanine / Q000378:metabolism; D010626:Phenylethyl Alcohol; D010710:Phosphates / Q000737:chemistry; D017680:Potassium Compounds / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
chemical_list: D003470:Culture Media; D010710:Phosphates; D017680:Potassium Compounds; D010649:Phenylalanine; C013216:potassium phosphate; D005947:Glucose; D010626:Phenylethyl Alcohol; D000645:Ammonium Sulfate