title: Host associated polymorphisms in the Corynebacterium pseudotuberculosis rpoB gene sequence.
abstract: Corynebacterium pseudotuberculosis is a widespread facultative intracellular pathogen that causes caseous lymphadenitis disease in sheep and goats, and generates cutaneous abscesses and granulomas in horses and cattle. Although some genes have been studied for diagnostic and phylogenetic analysis within the genus Corynebacterium, at subspecies level the pathogen has been poorly analyzed. The aim of this study was to characterize C. pseudotuberculosis strains isolated from domestic animals, through the sequencing of a hypervariable rpoB gene segment. As result, there were identified host associated rpoB polymorphisms in strains infecting sheep, goats and horses from Chile. These differences suggest the existence of bacterial genotypes, in which the nucleotide similarity values were ranging from 98.8 to 99.8%. In conclusion, the analysis of polymorphisms in the partial rpoB sequence can be used as a diagnostic tool that differentiates C. pseudotuberculosis strains at subspecies level.
authors.affiliation: Departamento de Medicina Preventiva Animal, Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile. Casilla 2, Correo 15, Santiago, Chile. pretamal@uchile.cl
authors.forename: Patricio
authors.initials: P
authors.lastname: Retamal
authors.forename: Marco
authors.initials: M
authors.lastname: Ríos
authors.forename: Felipe
authors.initials: F
authors.lastname: Cheuquepán
authors.forename: Pedro
authors.initials: P
authors.lastname: Abalos
authors.forename: José
authors.initials: J
authors.lastname: Pizarro-Lucero
authors.forename: Consuelo
authors.initials: C
authors.lastname: Borie
authors.forename: Josefina
authors.initials: J
authors.lastname: Gutierrez
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D002677:Chile; D003354:Corynebacterium Infections / Q000382:microbiology / Q000662:veterinary*; D016925:Corynebacterium pseudotuberculosis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005838:Genotype; D015511:Goat Diseases / Q000382:microbiology; D006041:Goats / Q000382:microbiology; D006734:Horse Diseases / Q000382:microbiology; D006736:Horses / Q000382:microbiology; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D012756:Sheep / Q000382:microbiology; D012757:Sheep Diseases / Q000382:microbiology; D013045:Species Specificity
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial