title: Junctional adhesion molecules are required for melanoma cell lines transendothelial migration in vitro.
abstract: One of the main steps of metastasis is extravasation, a phenomenon well described in lymphocytes but remaining to be fully uncovered for melanoma. Junctional adhesion molecules (JAMs) control the transendothelial migration of leukocytes. To date, the role of the JAM proteins, notably JAM-A and JAM-C, has not been examined in melanoma. Here, we compared two melanoma tumor cell lines, A375 and SLM8 cells, the A375 cell line being four times more efficient than the SLM8 cells in the crossing of the endothelial monolayer. We show evidence of the differential expression of JAM-A and JAM-C in these cell lines with JAM-C mainly expressed in the A375 cell line, and JAM-A detected preferentially in the SLM8 cells. To further dissect the respective roles of these proteins, we used both siRNA and blocking antibodies to decrease JAM-A and JAM-C expression.
authors.affiliation: Team 'Régulation des Réponses Immunitaires', Institut Jacques Monod CNRS-Université Paris Diderot, Bâtiment Buffon, 15 rue Hélène Brion, 75205 Paris, Cedex 13, France.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Ghislin
authors.forename: Dorian
authors.initials: D
authors.lastname: Obino
authors.forename: Sandrine
authors.initials: S
authors.lastname: Middendorp
authors.forename: Nicole
authors.initials: N
authors.lastname: Boggetto
authors.forename: Catherine
authors.initials: C
authors.lastname: Alcaide-Loridan
authors.forename: Frederique
authors.initials: F
authors.lastname: Deshayes
mesh_terms: D000912:Antibodies, Neoplasm / Q000494:pharmacology; D057134:Antibodies, Neutralizing / Q000494:pharmacology; D015815:Cell Adhesion Molecules / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement; D004730:Endothelium, Vascular / Q000378:metabolism* / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis* / Q000235:genetics; D008545:Melanoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000235:genetics; D011956:Receptors, Cell Surface
chemical_list: D000912:Antibodies, Neoplasm; D057134:Antibodies, Neutralizing; D015815:Cell Adhesion Molecules; C407243:F11R protein, human; D007136:Immunoglobulins; C448000:JAM3 protein, human; D009363:Neoplasm Proteins; D011956:Receptors, Cell Surface