title: Expression of β-catenin and cyclin D1 in epidermal stem cells of diabetic rats.
abstract: The healing of diabetic wounds represents a formidable clinical challenge, and the molecular mechanisms involved in diabetic wound healing are far from clear. In this study, we investigated the expression of β-catenin and cyclin D1 in the epidermal stem cells (ESCs) of diabetic rats, and explored whether the reduction of β-catenin and its downstream target in ESCs, cyclin D1, lead to poor wound healing in diabetes mellitus (DM). We found that, compared to the controls, the ESCs of diabetic rats were markedly reduced, the clone formation efficiency of the ESCs was markedly lower, and the mRNA and protein expression of β-catenin and cyclin D1 was significantly decreased. These findings suggest that the low expression of β-catenin and cyclin D1 may reduce the activity of ESCs from diabetic rats, which might be one of the important mechanisms of delayed wound healing in DM.
authors.affiliation: Burns Institute, Nanchang University Medical College, Nanchang, P.R. China.
authors.forename: Qing-Ling
authors.initials: QL
authors.lastname: Zhong
authors.forename: Fan-Rong
authors.initials: FR
authors.lastname: Liu
authors.forename: De-Wu
authors.initials: DW
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Peng
authors.forename: Xiang-Rong
authors.initials: XR
authors.lastname: Zhang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D048430:Cell Shape; D003114:Colony-Forming Units Assay; D019938:Cyclin D1 / Q000235:genetics / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D004817:Epidermis / Q000473:pathology*; D005786:Gene Expression Regulation; D007150:Immunohistochemistry; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000378:metabolism* / Q000473:pathology; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D051176:beta Catenin; D019938:Cyclin D1