title: Methodological approaches influencing cellular uptake and cyto-(geno) toxic effects of nanoparticles.
abstract: Methods are needed to assess cytotoxicity and genotoxicity of nanoparticles (NPs). The influence of serum and the use of cytochalasin-B were assessed on the cellular uptake of amorphous silica NPs (SNPs) and their biological effects. Our observations indicate that some methodological approaches may modulate the outcome of the assay. Therefore the experimental design and choice of the assays are of great importance in nanotoxicology.
authors.affiliation: Laboratory for Cellular Genetics, Vrije Universiteit Brussel, Pleinlaan 2, 1050 Brussels, Belgium.
authors.forename: Laetitia
authors.initials: L
authors.lastname: Gonzalez
authors.forename: Sara
authors.initials: S
authors.lastname: Corradi
authors.forename: Leen C J
authors.initials: LC
authors.lastname: Thomassen
authors.forename: Johan A
authors.initials: JA
authors.lastname: Martens
authors.forename: Enrico
authors.initials: E
authors.lastname: Cundari
authors.forename: Dominique
authors.initials: D
authors.lastname: Lison
authors.forename: Micheline
authors.initials: M
authors.lastname: Kirsch-Volders
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003603:Cytotoxins / Q000493:pharmacokinetics* / Q000633:toxicity*; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology / Q000503:physiopathology*; D008422:Materials Testing / Q000379:methods; D015162:Micronucleus Tests / Q000379:methods*; D053758:Nanoparticles / Q000633:toxicity*; D012822:Silicon Dioxide / Q000633:toxicity*; D036401:Toxicity Tests, Chronic / Q000379:methods*
chemical_list: D003603:Cytotoxins; D012822:Silicon Dioxide