title: Induction of pluripotent stem cells transplantation therapy for ischemic stroke.
abstract: Stroke can cause permanent neurological damage, complications, and even death. However, there is no treatment exists to restore its lost function. Human embryonic stems transplantation therapy was a novel and potential therapeutic approach for stroke. However, as we have seen, the ethical controversy pertains to embryonic stem cell research. Human induced pluripotent stem cells (iPSCs) are the latest generation of stem cells that may be a solution to the controversy of using embryonic cells. In our study, we generated iPSCs from adult human fibroblasts by introduction of four defined transcription factors (Oct4, Sox2, Nanog, and Lin-28). And then, we investigated the efficacy of iPSCs transplantation therapy for stroke on the animal models of middle cerebral artery occlusion. Surprisingly, we found that transplanted iPSCs migrated to injured brain areas, and differentiated into neuron-like cells successfully. After 4-16 days iPSCs grafting, sensorimotor function of rats has been improved significantly. In one word, we may prove that iPSCs therapy in stroke to be an effective form of treatment.
authors.affiliation: Department of Neurology, Shanghai Pudong New Area Gongli Hospital, 219 Miao Pu Road, Shanghai 200135, People's Republic of China.
authors.forename: Mei
authors.initials: M
authors.lastname: Jiang
authors.forename: Lei
authors.initials: L
authors.lastname: Lv
authors.forename: Haifeng
authors.initials: H
authors.lastname: Ji
authors.forename: Xuelian
authors.initials: X
authors.lastname: Yang
authors.forename: Wei
authors.initials: W
authors.lastname: Zhu
authors.forename: Liying
authors.initials: L
authors.lastname: Cai
authors.forename: Xiaju
authors.initials: X
authors.lastname: Gu
authors.forename: Changfeng
authors.initials: C
authors.lastname: Chai
authors.forename: Shu
authors.initials: S
authors.lastname: Huang
authors.forename: Jian
authors.initials: J
authors.lastname: Sun
authors.forename: Qiang
authors.initials: Q
authors.lastname: Dong
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000943:Antigens, Differentiation / Q000378:metabolism; D002545:Brain Ischemia / Q000628:therapy*; D002454:Cell Differentiation; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000473:pathology; D018920:Coculture Techniques; D004195:Disease Models, Animal; D005260:Female; D005347:Fibroblasts / Q000166:cytology; D020233:Gait Disorders, Neurologic / Q000628:therapy; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism / Q000637:transplantation*; D008297:Male; D008875:Middle Aged; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D020127:Recovery of Function; D020521:Stroke / Q000628:therapy*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014183:Transplantation, Heterologous
chemical_list: D000943:Antigens, Differentiation; D011994:Recombinant Proteins; D014157:Transcription Factors; D000469:Alkaline Phosphatase