title: Antibody-Hapten Recognition at the Surface of Functionalized Liposomes Studied by SPR: Steric Hindrance of Pegylated Phospholipids in Stealth Liposomes Prepared for Targeted Radionuclide Delivery.
abstract: Targeted PEGylated liposomes could increase the amount of drugs or radionuclides delivered to tumor cells. They show favorable stability and pharmacokinetics, but steric hindrance of the PEG chains can block the binding of the targeting moiety. Here, specific interactions between an antihapten antibody (clone 734, specific for the DTPA-indium complex) and DTPA-indium-tagged liposomes were characterized by surface plasmon resonance (SPR). Non-PEGylated liposomes fused on CM5 chips whereas PEGylated liposomes did not. By contrast, both PEGylated and non-PEGylated liposomes attached to L1 chips without fusion. SPR binding kinetics showed that, in the absence of PEG, the antibody binds the hapten at the surface of lipid bilayers with the affinity of the soluble hapten. The incorporation of PEGylated lipids hinders antibody binding to extents depending on PEGylated lipid fraction and PEG molecular weight. SPR on immobilized liposomes thus appears as a useful technique to optimize formulations of liposomes for targeted therapy.
authors.affiliation: Centre de Recherche en Cancérologie Nantes-Angers (CRCNA), Université de Nantes, Inserm, UMR 892, Institut de Recherche Thérapeutique de l'Université de Nantes, 8 quai Moncousu, BP 70721, 44007 Nantes Cedex 1, France.
authors.forename: Eliot P
authors.initials: EP
authors.lastname: Botosoa
authors.forename: Mike
authors.initials: M
authors.lastname: Maillasson
authors.forename: Marie
authors.initials: M
authors.lastname: Mougin-Degraef
authors.forename: Patricia
authors.initials: P
authors.lastname: Remaud-Le Saëc
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Gestin
authors.forename: Yannick
authors.initials: Y
authors.lastname: Jacques
authors.forename: Jacques
authors.initials: J
authors.lastname: Barbet
authors.forename: Alain
authors.initials: A
authors.lastname: Faivre-Chauvet