title: Hepatic immune response in calves during acute subclinical infection with bovine viral diarrhoea virus type 1.
abstract: Eight colostrum-deprived calves aged 8-12 weeks were inoculated intranasally with a non-cytopathic strain of bovine viral diarrhoea virus (BVDV) genotype-1 and the effects on the hepatic immune response were studied. Two calves were sacrificed at each of 3, 6, 9 and 14 days post-inoculation (dpi) and two uninoculated animals were used as negative controls. BVDV was detected in hepatic macrophages and monocytes from 3 to 14dpi and in Küpffer cells (KCs) from 6 to 14dpi. Increases in the numbers of MAC387(+) KCs and monocytes, but not interstitial macrophages, differentiated by morphological features, were evident in the liver following inoculation with BVDV. There was a substantial increase in the number of monocytes positive for tumour necrosis factor (TNF)-α, but only small increases in the numbers of TNF-α(+) KCs and interstitial macrophages and interleukin (IL)-6(+) monocytes, KCs and interstitial macrophages. There was an increase in the number of interstitial CD3(+) T lymphocytes in the liver, but no substantial changes in the numbers of circulating CD3(+) T lymphocytes, interstitial or circulating CD4(+) or CD8(+) T lymphocytes, or CD79αcy(+) B lymphocytes. Serum haptoglobin and serum amyloid A increased transiently at 12dpi. Upregulation of some pro-inflammatory cytokines by hepatic macrophages is evident in subclinical acute BVDV type 1 infection in calves.
authors.affiliation: Department of Comparative Pathology, Veterinary Faculty, University of Córdoba, Edificio Sanidad Animal, Campus de Rabanales, 14014 Córdoba, Spain.
authors.forename: M A
authors.initials: MA
authors.lastname: Risalde
authors.affiliation: Department of Comparative Pathology, Veterinary Faculty, University of Córdoba, Edificio Sanidad Animal, Campus de Rabanales, 14014 Córdoba, Spain.
authors.forename: J C
authors.initials: JC
authors.lastname: Gómez-Villamandos
authors.affiliation: Department of Comparative Pathology, Veterinary Faculty, University of Córdoba, Edificio Sanidad Animal, Campus de Rabanales, 14014 Córdoba, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Pedrera
authors.affiliation: Department of Comparative Pathology, Veterinary Faculty, University of Córdoba, Edificio Sanidad Animal, Campus de Rabanales, 14014 Córdoba, Spain.
authors.forename: V
authors.initials: V
authors.lastname: Molina
authors.affiliation: Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, University of Murcia, Espinardo 30100, Murcia, Spain.
authors.forename: J J
authors.initials: JJ
authors.lastname: Cerón
authors.affiliation: Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, University of Murcia, Espinardo 30100, Murcia, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Martínez-Subiela
authors.affiliation: Department of Comparative Pathology, Veterinary Faculty, University of Córdoba, Edificio Sanidad Animal, Campus de Rabanales, 14014 Córdoba, Spain. Electronic address: an2sacop@uco.es.
authors.forename: P J
authors.initials: PJ
authors.lastname: Sánchez-Cordón
mesh_terms: D000208:Acute Disease; D000209:Acute-Phase Proteins / Q000378:metabolism; D000818:Animals; D058345:Asymptomatic Infections; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000276:immunology* / Q000821:virology; D016022:Case-Control Studies; D002417:Cattle; D016207:Cytokines / Q000378:metabolism; D030241:Diarrhea Virus 1, Bovine Viral / Q000276:immunology* / Q000302:isolation & purification; D008099:Liver / Q000276:immunology* / Q000821:virology; D008264:Macrophages / Q000378:metabolism; D008297:Male; D009000:Monocytes / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism
chemical_list: D000209:Acute-Phase Proteins; D016207:Cytokines