title: Hybrid nanoparticle design based on cationized gelatin and the polyanions dextran sulfate and chondroitin sulfate for ocular gene therapy.
abstract: We describe the development of hybrid nanoparticles composed of cationized gelatin and the polyanions CS and DS for gene therapy in the ocular surface. The physicochemical properties of the nanoparticles that impact their bioperformance, such as average size and zeta potential, can be conveniently modulated by changing the ratio of polymers and the crosslinker. These systems associate plasmid DNA and are able to protect it from DNase I degradation. We corroborate that the introduction of CS or DS in the formulation decreases the in vitro toxicity of the nanoparticles to human corneal cells without compromising the transfection efficiency. These nanoparticles are potential candidates for the development of safer and more effective nanomedicines for ocular therapy.
authors.affiliation: Department of Pharmacy & Pharmaceutical Technology, Faculty of Pharmacy, University of Santiago de Compostela, Campus Sur 15782, Spain.
authors.forename: Giovanni Konat
authors.initials: GK
authors.lastname: Zorzi
authors.forename: Jenny Evelin
authors.initials: JE
authors.lastname: Párraga
authors.forename: Begoña
authors.initials: B
authors.lastname: Seijo
authors.forename: Alejandro
authors.initials: A
authors.lastname: Sánchez
mesh_terms: D000838:Anions; D002412:Cations / Q000737:chemistry; D002478:Cells, Cultured; D002809:Chondroitin Sulfates / Q000737:chemistry*; D003315:Cornea / Q000737:chemistry* / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D003850:Deoxyribonuclease I; D016264:Dextran Sulfate / Q000737:chemistry*; D005780:Gelatin / Q000737:chemistry*; D015316:Genetic Therapy; D006801:Humans; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010957:Plasmids / Q000235:genetics; D011108:Polymers / Q000737:chemistry; D014162:Transfection
chemical_list: D000838:Anions; D002412:Cations; D011108:Polymers; D005780:Gelatin; D002809:Chondroitin Sulfates; D004247:DNA; D016264:Dextran Sulfate; D003850:Deoxyribonuclease I