title: Transforming growth factor β1 promotes migration of human periodontal ligament cells through heat shock protein 27 phosphorylation.
abstract: Transforming growth factor β1 (TGF-β1) regulates cellular functions including cell growth, differentiation, and migration. However, signal transduction pathways of TGF-β1 are mostly undefined in human periodontal ligament (hPDL) cells. In this study, we investigated TGF β1-induced migration focusing on heat shock protein 27 (Hsp27) activation. The cellular response to TGF-β1 by hPDL cells was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and Western blot analysis. Cell migration was determined by counting migrated cells using a Chemotaxis Cell Migration Assay kit. TGF-β1 induced cell migration and increased the phosphorylation of Hsp27 and p38 mitogen-activated protein kinase (MAPK) in hPDL cells. Inhibiting p38 MAPK with SB203580 blocked TGF-β1-induced Hsp27 activation and cell migration. These findings suggest that TGF-β1-induced Hsp27 may promote the migration of hPDL cells via p38 MAPK mediated signaling. Hsp27 may be a potential target for the periodontal regeneration process related to cell migration.
authors.affiliation: Department of Pathology, Chosun University, School of Dentistry, Seoseok-dong, Dong-gu, Gwangju, Korea.
authors.forename: Seong-Min
authors.initials: SM
authors.lastname: Kwon
authors.forename: Soo-A
authors.initials: SA
authors.lastname: Kim
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Fujii
authors.forename: Hidefumi
authors.initials: H
authors.lastname: Maeda
authors.forename: Sang-Gun
authors.initials: SG
authors.lastname: Ahn
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Yoon
mesh_terms: D002465:Cell Movement; D055551:HSP27 Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology; D010513:Periodontal Ligament / Q000166:cytology* / Q000502:physiology; D010766:Phosphorylation; D011725:Pyridines / Q000494:pharmacology; D015398:Signal Transduction; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
chemical_list: D055551:HSP27 Heat-Shock Proteins; D007093:Imidazoles; D011725:Pyridines; D053773:Transforming Growth Factor beta1; D048051:p38 Mitogen-Activated Protein Kinases; C093642:SB 203580