title: Molecular cloning, characterization and expression profiles of three estrogen receptors in protogynous hermaphroditic orange-spotted grouper (Epinephelus coioides).
abstract: Estrogen plays key roles in vertebrate reproductive system via estrogen receptors (ERs) as mediating pathways. In the present study, three full-length ERs cDNA sequences were isolated from a protogynous teleost, the orange-spotted grouper (Epinephelus coioides), and were 2235bp for gERα, 1967bp for gERβ1 and 2158bp for gERβ2, respectively. Phylogenetic and amino acid alignment analyses showed that each gER was clustered in the corresponding taxonomic groups of the perciformes and exhibited high evolutional conservation in functional domains. RT-PCR revealed that gERs expressed at different levels in all the obtained tissues. gERα highly expressed in mature ovaries, gERβ1 mainly expressed in immature ovaries and gERβ2 varied greatly during ovarian development. During female to male sex reversal induced by 17α-methyltestosterone (MT) implantation, gERα decreased gradually, gERβ1 increased gradually, and gERβ2 decreased firstly and recovered subsequently in male stage. The present study speculated the potential roles of gERs during female maturation and female to male sex reversal induced by MT in the protogynous grouper E. coioides.
authors.affiliation: State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals, Sun Yat-Sen University, Guangzhou, Guangdong, China.
authors.forename: Huapu
authors.initials: H
authors.lastname: Chen
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Shuisheng
authors.initials: S
authors.lastname: Li
authors.forename: Miansheng
authors.initials: M
authors.lastname: Lin
authors.forename: Yu
authors.initials: Y
authors.lastname: Shi
authors.forename: Qing
authors.initials: Q
authors.lastname: Sang
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.forename: Haifa
authors.initials: H
authors.lastname: Zhang
authors.forename: Danqi
authors.initials: D
authors.lastname: Lu
authors.forename: Zining
authors.initials: Z
authors.lastname: Meng
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Liu
authors.forename: Haoran
authors.initials: H
authors.lastname: Lin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D017124:Conserved Sequence; D019143:Evolution, Molecular; D005260:Female; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects; D057850:Hermaphroditic Organisms / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D010053:Ovary / Q000378:metabolism; D010473:Perciformes / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D010802:Phylogeny; D011960:Receptors, Estrogen / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D012741:Sexual Maturation / Q000187:drug effects / Q000235:genetics
chemical_list: D029941:Fish Proteins; D011960:Receptors, Estrogen