title: Development of a metal-chelated plasmonic interface for the linking of His-peptides with a droplet-based surface plasmon resonance read-off scheme.
abstract: Monolayers of metal complexes were covalently attached to the surface of lamellar SPR interfaces (Ti/Ag/a-Si(0.63)C(0.37)) for binding histidine-tagged peptides with a controlled molecular orientation. The method is based on the activation of surface acid groups with N-hydroxysuccinimide (NHS), followed by an amidation reaction with (S)-N-(5-amino-1-carboxypentyl)iminodiacetic acid (NTA). FTIR and X-ray photoelectron spectroscopy (XPS) were used to characterize each surface modification step. The NTA modified SPR interface effectively chelated Cu(2+) ions. Once loaded with metal ions, the modified SPR interface was able to bind specifically to histidine-tagged peptides. The binding process was followed by surface plasmon resonance (SPR) in a droplet based configuration. The Cu(2+)-NTA modified interface showed protein loading comparable to commercially available NTA chips based on dextran chemistry and can thus be regarded as an interesting alternative. The sensor interface can be reused several times due to the easy regeneration step using ethylenediaminetetraacetic acid (EDTA) treatment.
authors.affiliation: Institut de Recherche Interdisciplinaire (USR 3078), Université Lille Nord de France, Parc de la Haute Borne, 50 Avenue de Halley, BP 70478, 59658 Villeneuve d'Ascq, France.
authors.forename: Nazek
authors.initials: N
authors.lastname: Maalouli
authors.forename: Anne Chantal
authors.initials: AC
authors.lastname: Gouget-Laemmel
authors.forename: Bernard
authors.initials: B
authors.lastname: Pinchemel
authors.forename: Mohamed
authors.initials: M
authors.lastname: Bouazaoui
authors.forename: Jean-Noël
authors.initials: JN
authors.lastname: Chazalviel
authors.forename: François
authors.initials: F
authors.lastname: Ozanam
authors.forename: Yongkun
authors.initials: Y
authors.lastname: Yang
authors.forename: Peter
authors.initials: P
authors.lastname: Burkhard
authors.forename: Rabah
authors.initials: R
authors.lastname: Boukherroub
authors.forename: Sabine
authors.initials: S
authors.lastname: Szunerits
mesh_terms: D000588:Amines / Q000737:chemistry; D000595:Amino Acid Sequence; D001920:Bradykinin / Q000737:chemistry / Q000378:metabolism; D002614:Chelating Agents / Q000737:chemistry*; D006639:Histidine / Q000737:chemistry*; D055767:Immobilized Proteins / Q000737:chemistry / Q000378:metabolism; D008670:Metals / Q000737:chemistry*; D008969:Molecular Sequence Data; D009571:Nitrilotriacetic Acid / Q000737:chemistry; D010455:Peptides / Q000737:chemistry*; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D000588:Amines; D002614:Chelating Agents; D055767:Immobilized Proteins; D008670:Metals; D010455:Peptides; D006639:Histidine; D009571:Nitrilotriacetic Acid; D001920:Bradykinin