title: Measurement of naturally acquired humoral immune responses against the C-terminal region of the Plasmodium vivax MSP1 protein using protein arrays.
abstract: Protein arrays are powerful tools for antibody profiling and vaccine development against infectious agents. In the previous report, we successfully applied an antibody-based protein array for immunoprofiling of Plasmodium vivax infection. Herein, we developed a Ni-NTA surface based protein array to detect immune responses against the recombinant C-terminal region (19 and 42 kDa) of the P. vivax merozoite surface protein 1 (PvMSP1-19 and -42) from sera of vivax malaria patients. The PvMSP1-19 arrays detected P. vivax in 112 of 130 (86.2%; 95% CI, 83.2-89.2%) microscopically positive samples and 2 false positives were obtained among 100 sera samples from healthy subjects (2.0%; 95% CI, 0.6-3.4%). These results were in concordance with results of enzyme-linked immunosorbent assays (ELISA). Kappa values represented excellent agreement for the recombinant PvMSP1-19 protein against sera samples as measured by protein arrays and ELISA (Kappa=0.904, 95% CI: 0.849-0.960). The PvMSP1-42 protein arrays detected antibody response in 100 of 130 microscopically positive samples (76.9%; 95% CI, 72.4-86.8%) and 8 false positives were obtained in 100 healthy subjects (8.0%; 95% CI, 2.7-13.3%). There is no significant difference between the fluorescent intensity of antibody response to PvMSP1-19 and PvMSP1-42 in the positive sera samples (P>0.05). The novel protein array platform may be used for profiling naturally acquired humoral immune responses to P. vivax infection.
authors.affiliation: Department of Parasitology and Institute of Medical Science, Kangwon National University School of Medicine, Hyoja2-dong, Chunchon, 200-701, Kangwon-do, Republic of Korea.
authors.forename: Jun-Hu
authors.initials: JH
authors.lastname: Chen
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
authors.forename: Kwon-Soo
authors.initials: KS
authors.lastname: Ha
authors.forename: Feng
authors.initials: F
authors.lastname: Lu
authors.forename: In-Bum
authors.initials: IB
authors.lastname: Suh
authors.forename: Chae Seung
authors.initials: CS
authors.lastname: Lim
authors.forename: Jeong Hyun
authors.initials: JH
authors.lastname: Park
authors.forename: Satoru
authors.initials: S
authors.lastname: Takeo
authors.forename: Takafumi
authors.initials: T
authors.lastname: Tsuboi
authors.forename: Eun-Taek
authors.initials: ET
authors.lastname: Han
mesh_terms: D000913:Antibodies, Protozoan / Q000097:blood*; D000953:Antigens, Protozoan / Q000276:immunology; D006801:Humans; D007158:Immunologic Techniques / Q000379:methods; D008288:Malaria / Q000276:immunology* / Q000469:parasitology; D020066:Merozoite Surface Protein 1 / Q000276:immunology*; D010966:Plasmodium vivax / Q000276:immunology*; D040081:Protein Array Analysis / Q000379:methods*
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D020066:Merozoite Surface Protein 1