title: Deletion of the virion host shut: off gene of pseudorabies virus results in selective upregulation of the expression of early viral genes in the late stage of infection.
abstract: A real-time RT-PCR technique was applied to evaluate the impact of deletion of the virion host shut-off (VHS) gene on the kinetics of pseudorabies virus gene expression. Selective suppression of early gene transcripts by the viral ribonuclease occurs after 4h of infection; while VHS protein appears to act non-selectively on the transcripts belonging in different kinetic classes in the first 2h of infection. VHS protein disrupts the close correlation between the transcription kinetics of the immediate-early 180 protein and the other pseudorabies virus transcripts. The typical pattern of early gene expression was found to be altered in the VHS gene-deleted virus in that the production rates of their transcripts did not decline from 4h post-infection. This observation led us to put forward the hypothesis that the VHS protein may play a pivotal role in the switch from the early to the late stage of infection.
authors.forename: Dóra
authors.initials: D
authors.lastname: Tombácz
authors.forename: Judit S
authors.initials: JS
authors.lastname: Tóth
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Boldogkoi
mesh_terms: D000818:Animals; D002460:Cell Line; D017353:Gene Deletion; D015967:Gene Expression Regulation, Viral; D011558:Herpesvirus 1, Suid / Q000235:genetics*; D007700:Kinetics; D012333:RNA, Messenger / Q000235:genetics; D012260:Ribonucleases / Q000235:genetics*; D013552:Swine; D014158:Transcription, Genetic; D015854:Up-Regulation; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D014764:Viral Proteins; D012260:Ribonucleases