title: Luminescence as a continuous real-time reporter of promoter activity in yeast undergoing respiratory oscillations or cell division rhythms.
abstract: This chapter describes a method for generating yeast respiratory oscillations in continuous culture and monitoring rhythmic promoter activity of the culture by automated real-time recording of luminescence. These techniques chiefly require the use of a strain of Saccharomyces cerevisiae that has been genetically modified to express firefly luciferase under the control of a promoter of interest and a continuous culture bioreactor that incorporates a photomultiplier apparatus for detecting light emission. Additionally, this chapter describes a method for observing rhythmic (cell cycle-related) promoter activity in small batch cultures of yeast through luminescence monitoring.
authors.affiliation: Department of Biological Sciences, Vanderbilt University, Nashville, TN, USA.
authors.forename: J Brian
authors.initials: JB
authors.lastname: Robertson
authors.forename: Carl Hirschie
authors.initials: CH
authors.lastname: Johnson
mesh_terms: D000332:Aerobiosis; D019149:Bioreactors / Q000382:microbiology; D018929:Cell Culture Techniques / Q000379:methods*; D002455:Cell Division / Q000235:genetics*; D002478:Cells, Cultured; D017930:Genes, Reporter / Q000235:genetics*; D049449:Luminescence; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000235:genetics* / Q000254:growth & development; D013997:Time Factors