title: The wag31 gene of Mycobacterium tuberculosis is positively regulated by the stringent response.
abstract: The stringent response of Mycobacterium tuberculosis is coordinated by Rel and is required for full virulence in animal models. A serological-based approach identified Wag31(Mtb) as a protein that is upregulated in M. tuberculosis in a rel-dependent manner. This positive regulation was confirmed by analysis of M. tuberculosis mRNA expression. Mycobacterium smegmatis was used to confirm that the expression of wag31(Mtb) from its native promoter is positively regulated by the stringent response. Furthermore, elevated wag31(Mtb) expression in M. smegmatis drastically alters the cell-surface hydrophobic properties.
authors.affiliation: Department of Biology, University of Minnesota Duluth, Duluth, MN, USA. jldahl@d.umn.edu
authors.forename: John L
authors.initials: JL
authors.lastname: Dahl
authors.forename: Dalia
authors.initials: D
authors.lastname: Lau Bonilla
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000378:metabolism; D020572:Oncogene Proteins v-rel / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D001426:Bacterial Proteins; D020572:Oncogene Proteins v-rel