title: Functions of a hemolysin-like protein in the cyanobacterium Synechocystis sp. PCC 6803.
abstract: A glucose-tolerant strain of the cyanobacterium Synechocystis sp. PCC 6803, generally referred to as wild type, produces a hemolysin-like protein (HLP) located on the cell surface. To analyze the function of HLP, we constructed a mutant in which the hlp gene was disrupted. The growth rate of the mutant was reduced when the cells were stressed by treatment with CuSO(4), CdCl(2), ZnCl(2), ampicillin, kanamycin, or sorbitol in liquid medium, suggesting that HLP may increase cellular resistance to the inhibitory effects of these compounds. Uptake assays with (109)Cd(2+) using the silicone-oil layer centrifugation technique revealed that both wild type and mutant cells were labeled with (109)Cd(2+) within 1 min. Although the total radioactivity was much higher in the wild-type cells, (109)Cd(2+) incorporation was clearly much higher in the mutant cells after adsorbed (109)Cd(2+) was removed from the cell surface by washing with EDTA. These findings suggest that HLP functions as a barrier against the adsorption of toxic compounds.
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, 305-8572, Japan.
authors.forename: Tetsushi
authors.initials: T
authors.lastname: Sakiyama
authors.forename: Hiroya
authors.initials: H
authors.lastname: Araie
authors.forename: Iwane
authors.initials: I
authors.lastname: Suzuki
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Shiraiwa
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002104:Cadmium / Q000378:metabolism; D048429:Cell Size; D003890:Desiccation; D006460:Hemolysin Proteins / Q000235:genetics / Q000378:metabolism*; D006358:Hot Temperature; D016254:Mutagenesis, Insertional; D013312:Stress, Physiological; D046939:Synechocystis / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D006460:Hemolysin Proteins; D002104:Cadmium