title: The effect of hydroxyapatite-coated titanium fiber web on human osteoblast functional activity.
abstract: OBJECTIVE
  The aim of this study was to evaluate human osteoblast activity on thin hydroxyapatite (HA)-coated three-dimensional scaffolds made of titanium fiber web.
  
  
  METHODS
  A thin HA film was coated on a titanium fiber web by the molecular precursor method. Human osteoblasts were disseminated onto the uncoated and HA-coated titanium fiber web, and osteoblast activity was observed at days 3, 7, 14, and 21 of culture.
  
  
  RESULTS
  Proliferation activities of osteoblasts were significantly higher in the uncoated titanium fiber web. Osteoblasts in the uncoated titanium fiber web showed a typical expression pattern, but those in the HA-coated titanium fiber web showed rapid osteocalcin expression and calcification at an early stage of culture. Moreover, osteocalcin expression per osteoblast was significantly higher in the HA-coated group.
  
  
  CONCLUSIONS
  These results suggest that HA coating with the molecular precursor method accelerated osteoblast functional activity.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Yokohama City University Graduate School of Medicine, Kanazawa-ku, Yokohama, Japan. mhirota@med.yokohama-cu.ac.jp
authors.forename: Makoto
authors.initials: M
authors.lastname: Hirota
authors.forename: Tohru
authors.initials: T
authors.lastname: Hayakawa
authors.forename: Akihiro
authors.initials: A
authors.lastname: Ametani
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Kuboki
authors.forename: Mitsunobu
authors.initials: M
authors.lastname: Sato
authors.forename: Iwai
authors.initials: I
authors.lastname: Tohnai
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis; D002113:Calcification, Physiologic / Q000502:physiology; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D048430:Cell Shape; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D024042:Collagen Type I / Q000032:analysis; D004396:Coloring Agents; D003470:Culture Media; D003764:Dental Materials / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D006801:Humans; D009499:Neutral Red; D010006:Osteoblasts / Q000502:physiology*; D015675:Osteocalcin / Q000032:analysis; D010641:Phenotype; D016062:Porosity; D013499:Surface Properties; D013997:Time Factors; D054457:Tissue Scaffolds / Q000737:chemistry*; D014025:Titanium / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D024042:Collagen Type I; D004396:Coloring Agents; D003470:Culture Media; D003764:Dental Materials; D015675:Osteocalcin; D009499:Neutral Red; D017886:Durapatite; D014025:Titanium; D000469:Alkaline Phosphatase