title: Manganese SOD mimics are effective against heat stress in a mutant fission yeast deficient in mitochondrial superoxide dismutase.
abstract: Previous studies revealed a close connection between heat shock and manganese-dependent superoxide dismutase (SOD2) in eukaryotes. This paper shows that SOD mimics based on manganese complexes caused an increase in thermotolerance for a mutant fission yeast deficient in mitochondrial superoxide dismutase. Manganese compounds used for tests are SOD mimics, from two different classes: salen manganese (EUK-8) and Mn porphyrin (Mn(III)TE-2-PyP(5+)). The tests were conducted using a Schizosaccharomyces pombe model, comparing the viability of two strains at chronic heat stress (37°C)--a wild type versus a strain with the mitochondrial superoxide dismutase gene deleted [SOD2(-)]. The presence of massive free radical species in S. pombe SOD2(-) was demonstrated using a luminol-enhanced chemiluminescence test derived from a menadione-mediated survival protocol.
  
  
  CONCLUSIONS
  Survival tests revealed that the SOD2-deleted S. pombe is about 100 times more sensitive to heat stress than the wild-type strain. This survival deficit can be corrected by EUK-8 and Mn(III)TE-2-PyP(5+) to almost the same degree but not by manganese chloride II (MnCl(2)). Using a simple spot assay for viability testing, this new model proved to be an easy alternative for the initial estimation of manganese SOD mimics efficiency.
authors.affiliation: Department of Biochemistry, Gr. T. Popa University of Medicine and Pharmacy, Universitatii 16, Iasi, 700115, Romania. bastoica@mail.umfiasi.ro
authors.forename: Bogdan Alexandru
authors.initials: BA
authors.lastname: Stoica
authors.forename: Mircea
authors.initials: M
authors.lastname: Rusu
authors.forename: Tudor
authors.initials: T
authors.lastname: Petreus
authors.forename: Mihai
authors.initials: M
authors.lastname: Nechifor
mesh_terms: D003470:Culture Media; D016166:Free Radical Scavengers / Q000494:pharmacology*; D017353:Gene Deletion; D006358:Hot Temperature; D049449:Luminescence; D008928:Mitochondria / Q000378:metabolism*; D018716:Molecular Mimicry; D019255:NADPH Oxidases / Q000378:metabolism; D016877:Oxidants / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D012568:Schizosaccharomyces / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics; D013312:Stress, Physiological / Q000187:drug effects*; D013482:Superoxide Dismutase / Q000172:deficiency / Q000235:genetics / Q000494:pharmacology*; D013481:Superoxides / Q000378:metabolism; D024483:Vitamin K 3 / Q000494:pharmacology
chemical_list: D003470:Culture Media; D016166:Free Radical Scavengers; D016877:Oxidants; D017382:Reactive Oxygen Species; D013481:Superoxides; D024483:Vitamin K 3; D013482:Superoxide Dismutase; D019255:NADPH Oxidases