title: Statistical analysis of features associated with protein expression/solubility in an in vivo Escherichia coli expression system and a wheat germ cell-free expression system.
abstract: Recombinant protein technology is an important tool in many industrial and pharmacological applications. Although the success rate of obtaining soluble proteins is relatively low, knowledge of protein expression/solubility under 'standard' conditions may increase the efficiency and reduce the cost of proteomics studies. In this study, we conducted a genome-scale experiment to assess the overexpression and the solubility of human full-length cDNA in an in vivo Escherichia coli expression system and a wheat germ cell-free expression system. We evaluated the influences of sequence and structural features on protein expression/solubility in each system and estimated a minimal set of features associated with them. A comparison of the feature sets related to protein expression/solubility in the in vivo Escherichia coli expression system revealed that the structural information was strongly associated with protein expression, rather than protein solubility. Moreover, a significant difference was found in the number of features associated with protein solubility in the two expression systems.
authors.affiliation: Computational Biology Research Center (CBRC), National Institute of Advanced Industrial Science and Technology (AIST), Tokyo, Japan. hirose-shuichi@aist.go.jp
authors.forename: Shuichi
authors.initials: S
authors.lastname: Hirose
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Kawamura
authors.forename: Kiyonobu
authors.initials: K
authors.lastname: Yokota
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Kuroita
authors.forename: Tohru
authors.initials: T
authors.lastname: Natsume
authors.forename: Kazuo
authors.initials: K
authors.lastname: Komiya
authors.forename: Takeshi
authors.initials: T
authors.lastname: Tsutsumi
authors.forename: Yorimasa
authors.initials: Y
authors.lastname: Suwa
authors.forename: Takao
authors.initials: T
authors.lastname: Isogai
authors.forename: Naoki
authors.initials: N
authors.lastname: Goshima
authors.forename: Tamotsu
authors.initials: T
authors.lastname: Noguchi
mesh_terms: D002474:Cell-Free System / Q000378:metabolism*; D018076:DNA, Complementary / Q000235:genetics; D003627:Data Interpretation, Statistical; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D014176:Protein Biosynthesis; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification; D012995:Solubility; D014908:Triticum / Q000235:genetics / Q000378:metabolism*
chemical_list: D018076:DNA, Complementary; D011994:Recombinant Proteins