title: Complex interactions between GSK3 and aPKC in Drosophila embryonic epithelial morphogenesis.
abstract: Generally, epithelial cells must organize in three dimensions to form functional tissue sheets. Here we investigate one such sheet, the Drosophila embryonic epidermis, and the morphogenetic processes organizing cells within it. We report that epidermal morphogenesis requires the proper distribution of the apical polarity determinant aPKC. Specifically, we find roles for the kinases GSK3 and aPKC in cellular alignment, asymmetric protein distribution, and adhesion during the development of this polarized tissue. Finally, we propose a model explaining how regulation of aPKC protein levels can reorganize both adhesion and the cytoskeleton.
authors.affiliation: Program in Developmental Biology, Sloan-Kettering Institute, Memorial Sloan-Kettering Cancer Center, New York, New York, United States of America.
authors.forename: Nicole A
authors.initials: NA
authors.lastname: Kaplan
authors.forename: Pamela F
authors.initials: PF
authors.lastname: Colosimo
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Liu
authors.forename: Nicholas S
authors.initials: NS
authors.lastname: Tolwinski
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D016764:Cell Polarity; D004331:Drosophila melanogaster / Q000166:cytology / Q000196:embryology* / Q000201:enzymology* / Q000235:genetics; D004625:Embryo, Nonmammalian / Q000166:cytology*; D000078404:Epidermal Cells; D004847:Epithelial Cells / Q000166:cytology* / Q000201:enzymology* / Q000378:metabolism; D005260:Female; D038362:Glycogen Synthase Kinase 3 / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D008297:Male; D008957:Models, Genetic; D010766:Phosphorylation; D011485:Protein Binding; D011493:Protein Kinase C / Q000378:metabolism*; D021381:Protein Transport; D015398:Signal Transduction; D051153:Wnt Proteins / Q000378:metabolism
chemical_list: D051153:Wnt Proteins; C115015:PKC-3 protein; D011493:Protein Kinase C; D038362:Glycogen Synthase Kinase 3