title: Human dental pulp progenitor cell behavior on aqueous and hexafluoroisopropanol based silk scaffolds.
abstract: Silk scaffolds have been successfully used for a variety of tissue engineering applications due to their biocompatibility, diverse physical characteristics, and ability to support cell attachment and proliferation. Our prior characterization of 4-day postnatal rat tooth bud cells grown on hexafluoro-2-propanol (HFIP) silk scaffolds showed that the silk scaffolds not only supported osteodentin formation, but also guided the size and shape of the formed osteodentin. In this study, interactions between human dental pulp cells and HFIP and aqueous based silk scaffolds were studied under both in vitro and in vivo conditions. Silk scaffold porosity and incorporation of RGD and DMP peptides were examined. We found that the degradation of aqueous based silk is much faster than HFIP based silk scaffolds. Also, HFIP based silk scaffolds supported the soft dental pulp formation better than the aqueous based silk scaffolds. No distinct hard tissue regeneration was found in any of the implants, with or without additional cells. We conclude that alternative silk scaffold materials, and hDSC pre-seeding cell treatments or sorting and enrichment methods, need to be considered for successful dental hard tissue regeneration.
authors.affiliation: Division of Craniofacial and Molecular Genetics, Department of Oral and Maxillofacial Pathology, Tufts University School of Dental Medicine, Boston, Massachusetts, USA.
authors.forename: Weibo
authors.initials: W
authors.lastname: Zhang
authors.forename: Ivy Pruitt
authors.initials: IP
authors.lastname: Ahluwalia
authors.forename: Robert
authors.initials: R
authors.lastname: Literman
authors.forename: David L
authors.initials: DL
authors.lastname: Kaplan
authors.forename: Pamela C
authors.initials: PC
authors.lastname: Yelick
mesh_terms: D000293:Adolescent; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects; D002113:Calcification, Physiologic / Q000187:drug effects; D002469:Cell Separation; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology*; D005260:Female; D005452:Fluoresceins / Q000378:metabolism; D006801:Humans; D020007:Implants, Experimental; D008297:Male; D018919:Neovascularization, Physiologic / Q000187:drug effects; D009842:Oligopeptides / Q000494:pharmacology; D010750:Phosphoproteins / Q000494:pharmacology; D020005:Propanols / Q000494:pharmacology*; D019919:Prosthesis Implantation; D051381:Rats; D011923:Rats, Nude; D047011:Silk / Q000494:pharmacology*; D013234:Stem Cells / Q000166:cytology*; D017154:Stromal Cells / Q000166:cytology / Q000187:drug effects; D054457:Tissue Scaffolds / Q000737:chemistry*; D014867:Water / Q000494:pharmacology*; D006845:Hydrocarbons, Fluorinated
chemical_list: D005452:Fluoresceins; D009842:Oligopeptides; D010750:Phosphoproteins; D020005:Propanols; D047011:Silk; D014867:Water; C001337:hexafluoroisopropanol; D006845:Hydrocarbons, Fluorinated; C047981:arginyl-glycyl-aspartic acid; C007740:fluorexon