title: Noninvasive bioluminescent quantification of viable stem cells in engineered constructs.
abstract: Bioluminescence from murine stem cells tagged with the luciferase gene reporter and distributed within three-dimensional scaffolds of two different materials is quantified in vitro and in vivo. The luminescence emitted from cells adhering to the scaffolds tested is monitored noninvasively using a bioluminescence imaging system. Monitoring the kinetics of luciferase expression via bioluminescence enables real-time assessment of cell survival and proliferation on scaffolds both in vitro and in vivo over prolonged (8 weeks) periods of time.
authors.affiliation: Laboratoire de Bio-ingéniérie et Biomécanique Ostéo-articulaires, UMR, CNRS, 7052 Paris, France.
authors.forename: Karim
authors.initials: K
authors.lastname: Oudina
authors.forename: Adeline
authors.initials: A
authors.lastname: Cambon-Binder
authors.forename: Delphine
authors.initials: D
authors.lastname: Logeart-Avramoglou
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D002470:Cell Survival; D021621:Imaging, Three-Dimensional; D020007:Implants, Experimental; D008163:Luminescent Measurements / Q000379:methods*; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D051379:Mice; D008819:Mice, Nude; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D051598:Whole Body Imaging