title: A protocol to assess cell cycle and apoptosis in human and mouse pluripotent cells.
abstract: Embryonic stem cells (ESC) and induced pluripotent stem cells (iPSCs) present a great opportunity to treat and model human disease as a cell replacement therapy. There is a growing pressure to understand better the signal transduction pathways regulating pluripotency and self-renewal of these special cells in order to deliver a safe and reliable cell based therapy in the near future. Many signal transduction pathways converge on two major cell functions associated with self-renewal and pluripotency: control of the cell cycle and apoptosis, although a standard method is lacking across the field. Here we present a detailed protocol to assess the cell cycle and apoptosis of ESC and iPSCs as a single reference point offering an easy to use standard approach across the field.
authors.affiliation: Center of Regenerative Medicine in Barcelona, Dr, Aiguader 88, 08003 Barcelona, Spain. belmonte@salk.edu.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Edel
authors.forename: Cristina
authors.initials: C
authors.lastname: Menchon
authors.forename: Jose Miguel Andres
authors.initials: JM
authors.lastname: Vaquero
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Izpisua Belmonte