title: Prolactin-releasing peptide mRNA expression in mouse medulla remains relatively stable during pregnancy and lactation.
abstract: We compared levels of prolactin-releasing peptide (PrRP) mRNA expression in mouse medulla at different stages of pregnancy and lactation. Mouse medulla samples were collected on days 6, 12 and 18 of pregnancy and lactation, respectively (six per group), for mRNA. Expression levels of PrRP mRNA in the medulla were measured by semi-quantitative RT-PCR, with glyceraldehyde 3-phosphate dehydrogenase as a control. PrRP mRNA was highly expressed in mouse medulla oblongata on day 6 of pregnancy (0.53), followed by 0.43 at lactation day 6, and 0.42 at lactation day 12. The expression level of PrRP mRNA on days 12 and 18 of pregnancy and day 18 of lactation shared the same value of 0.36. PrRP mRNA levels during lactation decreased slightly compared with that during pregnancy, but the differences between them were not significant. In summary, PrRP mRNA levels in the medulla oblongata remain relatively stable during pregnancy and lactation. This is evidence that medulla PrRP is not involved in the regulation of prolactin secretion.
authors.affiliation: Key Laboratory of Regulation on Animal Growth and Development, Agricultural Ministry of China, Henan Agricultural University, Zhengzhou, PR China. zhuheshui@163.com
authors.forename: H S
authors.initials: HS
authors.lastname: Zhu
authors.forename: J X
authors.initials: JX
authors.lastname: Du
authors.forename: Y Y
authors.initials: YY
authors.lastname: Wang
authors.forename: L F
authors.initials: LF
authors.lastname: Wang
authors.forename: L Q
authors.initials: LQ
authors.lastname: Hang
authors.forename: G Y
authors.initials: GY
authors.lastname: Yang
authors.forename: Y L
authors.initials: YL
authors.lastname: Wang
mesh_terms: D000818:Animals; D005260:Female; D015870:Gene Expression; D024601:Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+) / Q000235:genetics; D007774:Lactation; D008526:Medulla Oblongata / Q000166:cytology / Q000201:enzymology / Q000378:metabolism; D051379:Mice; D010908:Pituitary Hormones, Anterior / Q000378:metabolism; D011247:Pregnancy; D011388:Prolactin / Q000378:metabolism; D056690:Prolactin-Releasing Hormone / Q000096:biosynthesis* / Q000235:genetics*; D012333:RNA, Messenger; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D010908:Pituitary Hormones, Anterior; D056690:Prolactin-Releasing Hormone; D012333:RNA, Messenger; D011388:Prolactin; D024601:Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+)