title: Effect of ditaxin and heteranthin and inhibitory effect of Ditaxis heterantha extract on L5178Y tumor development in mice.
abstract: Ditaxis heterantha seeds are used as spices for flavoring and coloring food. Two new apocarotenoids derived from the seeds, heteranthin and ditaxin, were evaluated for their in vitro cytotoxic effects in murine lymphoma cells lines. Bioabsorption in mice and preventive and antitumor effects of the apocarotenoids were determined. Ditaxin and heteranthin showed cytotoxic effects in vitro against murine malignant cells and normal splenocyte cells. The 50% inhibitory concentration (IC(50)) for ditaxin in splenocytes was 0.1825 mM; in L5178Y, the IC(50) was 0.1923 mM. The heteranthin IC(50) in splenocytes was 0.1325 mM; in L5178Y, the value was 0.3889 mM. The maximum ditaxin plasma concentration was found after 2 hours of administration (mean±standard deviation, 7.5±2.05 μg/mL). Oral administration of the D. heterantha extract (100 mg/kg per day) for 14 days after the L5178Y lymphoma cell implantation showed no significant effect compared with groups that were not pretreated. However, tumor inhibition in groups treated intraperitoneally before inoculation with the L5178Y cells showed a significant difference (P<.001) compared with the groups not pretreated.
authors.affiliation: Department of Food Science, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City, Mexico. jaramillo_flores@hotmail.com
authors.forename: María Eugenia
authors.initials: ME
authors.lastname: Jaramillo-Flores
authors.forename: Herry Heriyati
authors.initials: HH
authors.lastname: Permady
authors.forename: Ana Maria
authors.initials: AM
authors.lastname: Puebla-Pérez
authors.forename: Eduardo
authors.initials: E
authors.lastname: Padilla
authors.forename: Eugenia del Carmen
authors.initials: Edel C
authors.lastname: Lugo-Cervantes
authors.forename: Jorge Ivan
authors.initials: JI
authors.lastname: Delgado-Saucedo
authors.forename: Eva
authors.initials: E
authors.lastname: Ramón-Gallegos
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000493:pharmacokinetics*; D002338:Carotenoids / Q000008:administration & dosage / Q000493:pharmacokinetics*; D045744:Cell Line, Tumor; D019665:Euphorbiaceae / Q000737:chemistry; D005260:Female; D006801:Humans; D020128:Inhibitory Concentration 50; D008223:Lymphoma / Q000188:drug therapy; D051379:Mice; D008807:Mice, Inbred BALB C; D009374:Neoplasms, Experimental / Q000188:drug therapy*; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000493:pharmacokinetics*; D012639:Seeds / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D010936:Plant Extracts; C513757:ditaxin; C513756:heteranthin; D002338:Carotenoids