title: Semi-quantitative analyses of metabolic systems of human colon cancer metastatic xenografts in livers of superimmunodeficient NOG mice.
abstract: Analyses of energy metabolism in human cancer have been difficult because of rapid turnover of the metabolites and difficulties in reducing time for collecting clinical samples under surgical procedures. Utilization of xenograft transplantation of human-derived colon cancer HCT116 cells in spleens of superimmunodeficient NOD/SCID/IL-2Rγ(null) (NOG) mice led us to establish an experimental model of hepatic micrometastasis of the solid tumor, whereby analyses of the tissue sections collected by snap-frozen procedures through newly developed microscopic imaging mass spectrometry (MIMS) revealed distinct spatial distribution of a variety of metabolites. To perform intergroup comparison of the signal intensities of metabolites among different tissue sections collected from mice in fed states, we combined matrix-assisted laser desorption/ionization time-of-flight imaging mass spectrometry (MALDI-TOF-IMS) and capillary electrophoresis-mass spectrometry (CE-MS), to determine the apparent contents of individual metabolites in serial tissue sections. The results indicated significant elevation of ATP and energy charge in both metastases and the parenchyma of the tumor-bearing livers. To note were significant increases in UDP-N-acetyl hexosamines, and reduced and oxidized forms of glutathione in the metastatic foci versus the liver parenchyma. These findings thus provided a potentially important method for characterizing the properties of metabolic systems of human-derived cancer and the host tissues in vivo.
authors.affiliation: Department of Biochemistry, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo, 160-8582, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Kubo
authors.forename: Mitsuyo
authors.initials: M
authors.lastname: Ohmura
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Wakui
authors.forename: Takahiro
authors.initials: T
authors.lastname: Harada
authors.forename: Shigeki
authors.initials: S
authors.lastname: Kajihara
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Ogawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Suemizu
authors.forename: Masato
authors.initials: M
authors.lastname: Nakamura
authors.forename: Mitsutoshi
authors.initials: M
authors.lastname: Setou
authors.forename: Makoto
authors.initials: M
authors.lastname: Suematsu
mesh_terms: D000818:Animals; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology*; D004195:Disease Models, Animal; D019075:Electrophoresis, Capillary; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism / Q000556:secondary*; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014183:Transplantation, Heterologous