title: Biomarker candidates of Chlamydophila pneumoniae proteins and protein fragments identified by affinity-proteomics using FTICR-MS and LC-MS/MS.
abstract: We report here an affinity-proteomics approach that combines 2D-gel electrophoresis and immunoblotting with high performance mass spectrometry to the identification of both full length protein antigens and antigenic fragments of Chlamydophila pneumoniae (C. pneumoniae). The present affinity-mass spectrometry approach effectively utilized high resolution FTICR mass spectrometry and LC-tandem-MS for protein identification, and enabled the identification of several new highly antigenic C. pneumoniae proteins that were not hitherto reported or previously detected only in other Chlamydia species, such as Chlamydia trachomatis. Moreover, high resolution affinity-MS provided the identification of several neo-antigenic protein fragments containing N- and C-terminal, and central domains such as fragments of the membrane protein Pmp21 and the secreted chlamydial proteasome-like factor (Cpaf), representing specific biomarker candidates.
authors.affiliation: Laboratory of Analytical Chemistry and Biopolymer Structure Analysis, Department of Chemistry, University of Konstanz, 78457 Konstanz, Germany.
authors.forename: Iuliana
authors.initials: I
authors.lastname: Susnea
authors.forename: Sebastian
authors.initials: S
authors.lastname: Bunk
authors.forename: Albrecht
authors.initials: A
authors.lastname: Wendel
authors.forename: Corinna
authors.initials: C
authors.lastname: Hermann
authors.forename: Michael
authors.initials: M
authors.lastname: Przybylski
mesh_terms: D000942:Antigens, Bacterial / Q000737:chemistry; D001426:Bacterial Proteins / Q000737:chemistry*; D015415:Biomarkers / Q000737:chemistry; D016993:Chlamydophila pneumoniae / Q000737:chemistry / Q000378:metabolism*; D007118:Immunoassay; D010446:Peptide Fragments / Q000737:chemistry*; D040901:Proteomics / Q000379:methods*; D017550:Spectroscopy, Fourier Transform Infrared; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D015415:Biomarkers; D010446:Peptide Fragments