title: The role of the lignan constituents in the effect of Schisandra chinensis fruit extract on penile erection.
abstract: The effect of the ethanol extract and active components of the fruit of Schisandra chinensis was evaluated on rabbit penile corpus cavernosum (PCC). PCC, pre-contracted with 10(-5)  m phenylephrine (Phe), was treated with extracts of S. chinensis at five different compositions of ethanol and water (95%, 70%, 50%, 30% and ethanol/water [v/v]) and three fractions (0.1, 0.5, 1 and 2 mg/mL). The effect of the extracts and active lignans (schisandrol A and schisandrol B) from S. chinensis on sildenafil citrate pre-incubated PCC was also evaluated. The PCC relaxation induced by the 95% ethanol extraction and the n-hexane fraction was concentration-dependent and the ethanol extract enhanced sildenafil citrate-induced PCC relaxation. The active components of S. chinensis (schisandrol A and schisandrol B) significantly enhanced sildenafil citrate-induced relaxation >2-fold; schisandrol A had the highest relaxant effect on sildenafil citrate pre-incubated PCC. The lignans, schisandrol A and schisandrol B, isolated from the fruits of S. chinensis enhanced sildenafil citrate-induced relaxation and may have synergistic action in patients who do not completely respond to sildenafil.
authors.affiliation: Department of Urology, Medical School, and Institute for Medical Sciences, Chonbuk National University, and Research Institute and CTC of Medical Device of Chonbuk National University Hospital, Jeonju, 561-712, Republic of Korea.
authors.forename: Hye Kyung
authors.initials: HK
authors.lastname: Kim
authors.forename: Yun Ok
authors.initials: YO
authors.lastname: Bak
authors.forename: Bo Ram
authors.initials: BR
authors.lastname: Choi
authors.forename: Chen
authors.initials: C
authors.lastname: Zhao
authors.forename: Hee Ju
authors.initials: HJ
authors.lastname: Lee
authors.forename: Chul Young
authors.initials: CY
authors.lastname: Kim
authors.forename: Sung Won
authors.initials: SW
authors.lastname: Lee
authors.forename: Ju Hong
authors.initials: JH
authors.lastname: Jeon
authors.forename: Jong Kwan
authors.initials: JK
authors.lastname: Park
mesh_terms: D000818:Animals; D034242:Cyclooctanes; D004149:Dioxoles; D005638:Fruit / Q000737:chemistry; D066298:In Vitro Techniques; D017705:Lignans / Q000494:pharmacology*; D008297:Male; D009126:Muscle Relaxation / Q000187:drug effects*; D009130:Muscle, Smooth / Q000187:drug effects; D010410:Penile Erection / Q000187:drug effects*; D010879:Piperazines / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology*; D011687:Purines / Q000494:pharmacology; D011817:Rabbits; D032141:Schisandra / Q000737:chemistry*; D000068677:Sildenafil Citrate; D013450:Sulfones / Q000494:pharmacology
chemical_list: D034242:Cyclooctanes; D004149:Dioxoles; D017705:Lignans; D010879:Piperazines; D010936:Plant Extracts; D011687:Purines; D013450:Sulfones; C520474:schisandrol A; C033585:schizandrol B; D000068677:Sildenafil Citrate