title: Auxin modulates the enhanced development of root hairs in Arabidopsis thaliana (L.) Heynh. under elevated CO(2).
abstract: Root hairs may play a critical role in nutrient acquisition of plants grown under elevated CO(2) . This study investigated how elevated CO(2) enhanced the development of root hairs in Arabidopsis thaliana (L.) Heynh. The plants under elevated CO(2) (800 µL L(-1)) had denser and longer root hairs, and more H-positioned cells in root epidermis than those under ambient CO(2) (350 µL L(-1)). The elevated CO(2) increased auxin production in roots. Under elevated CO(2) , application of either 1-naphthoxyacetic acid (1-NOA) or N-1-naphthylphthalamic acid (NPA) blocked the enhanced development of root hairs. The opposite was true when the plants under ambient CO(2) were treated with 1-naphthylacetic acid (NAA), an auxin analogue. Furthermore, the elevated CO(2) did not enhance the development of root hairs in auxin-response mutants, axr1-3, and auxin-transporter mutants, axr4-1, aux1-7 and pin1-1. Both elevated CO(2) and NAA application increased expressions of caprice, triptychon and rho-related protein from plants 2, and decreased expressions of werewolf, GLABRA2, GLABRA3 and the transparent testa glabra 1, genes related to root-hair development, while 1-NOA and NPA application had an opposite effect. Our study suggests that elevated CO(2) enhanced the development of root hairs in Arabidopsis via the well-characterized auxin signalling and transport that modulate the initiation of root hairs and the expression of its specific genes.
authors.affiliation: Ministry of Education Key Laboratory of Environmental Remediation and Ecosystem Health, College of Environmental and Resources Sciences, China.
authors.forename: Yaofang
authors.initials: Y
authors.lastname: Niu
authors.forename: Chongwei
authors.initials: C
authors.lastname: Jin
authors.forename: Gulei
authors.initials: G
authors.lastname: Jin
authors.forename: Qingyan
authors.initials: Q
authors.lastname: Zhou
authors.forename: Xianyong
authors.initials: X
authors.lastname: Lin
authors.forename: Caixian
authors.initials: C
authors.lastname: Tang
authors.forename: Yongsong
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D017360:Arabidopsis / Q000254:growth & development* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics; D002245:Carbon Dioxide / Q000494:pharmacology*; D004268:DNA-Binding Proteins / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D006016:Glycolates / Q000494:pharmacology; D018398:Homeodomain Proteins / Q000235:genetics; D007210:Indoleacetic Acids / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D009280:Naphthaleneacetic Acids / Q000494:pharmacology; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D010797:Phthalimides / Q000494:pharmacology; D019441:Plant Epidermis / Q000166:cytology / Q000187:drug effects; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D020598:Proto-Oncogene Proteins c-myb / Q000235:genetics; D015398:Signal Transduction; D014157:Transcription Factors
chemical_list: D029681:Arabidopsis Proteins; D051792:Basic Helix-Loop-Helix Proteins; D002245:Carbon Dioxide; D004268:DNA-Binding Proteins; D006016:Glycolates; D018398:Homeodomain Proteins; D007210:Indoleacetic Acids; D026901:Membrane Transport Proteins; D009280:Naphthaleneacetic Acids; D009687:Nuclear Proteins; D010797:Phthalimides; D020598:Proto-Oncogene Proteins c-myb; D014157:Transcription Factors; C081977:AXR1 protein, Arabidopsis; C110728:AXR3 protein, Arabidopsis; C511989:CPC protein, Arabidopsis; C089991:GL2 protein, Arabidopsis; C417284:GL3 protein, Arabidopsis; C116363:PIN1 protein, Arabidopsis; C468526:TRIPTYCHON protein, Arabidopsis; C121067:TTG1 protein, Arabidopsis; C402111:WER protein, Arabidopsis; C002687:alpha-naphthylphthalamic acid; C034182:1-naphthaleneacetic acid; C018610:1-naphthoxyacetic acid