title: Effects of isoflurane on Nfκb p65, Gadd45a and Jnk1 expression in the vital organs of CBA/CA mice.
abstract: BACKGROUND
  Isoflurane is administered to patients during general anaesthesia to diminish the effects of surgical invasion.
  
  
  METHODS
  CBA/CA mice were exposed to 2% isoflurane during general anaesthesia. Gene expressions were measured 6 and 12 hours later by quantitative real-time PCR on total RNA isolated from the lung, liver and kidneys of the animals. Expressions of growth arrest and DNA-damage-inducible 45 alpha (Gadd45 α), Jun N-terminal kinase 1 (Jnk1) and nuclear factor of kappa light polypeptide gene enhancer in B-cells p65 subunit (Nfκb p65) were analyzed.
  
  
  RESULTS
  Gadd45α and Jnk1 showed significant inverse expression changes in the different tissues compared to the Nfκb p65. The length of anaesthesia also modified the gene induction.
  
  
  CONCLUSIONS
  Isoflurane has significant modulatory effect on the transcription of genes regulating inflammation and apoptotic signalling.
authors.affiliation: Anaesthesiology and Intensive Care Unit, BAZ County Teaching Hospital, Miskolc, Hungary. balazs.kadar@airambulance.hu
authors.forename: Balázs
authors.initials: B
authors.lastname: Kádár
authors.forename: Katalin
authors.initials: K
authors.lastname: Gombos
authors.forename: Eszter
authors.initials: E
authors.lastname: Szele
authors.forename: István
authors.initials: I
authors.lastname: Ember
authors.forename: János László
authors.initials: JL
authors.lastname: Iványi
authors.forename: Rás
authors.initials: R
authors.lastname: Csejtei
authors.forename: Gábor
authors.initials: G
authors.lastname: Pajkos
mesh_terms: D018685:Anesthetics, Inhalation / Q000494:pharmacology; D000818:Animals; D018797:Cell Cycle Proteins / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D007530:Isoflurane / Q000494:pharmacology*; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008168:Lung / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008808:Mice, Inbred CBA; D048055:Mitogen-Activated Protein Kinase 8 / Q000235:genetics*; D009687:Nuclear Proteins / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D051996:Transcription Factor RelA / Q000235:genetics*; D000097642:GADD45 Proteins
chemical_list: D018685:Anesthetics, Inhalation; D018797:Cell Cycle Proteins; D007530:Isoflurane; D048055:Mitogen-Activated Protein Kinase 8; D009687:Nuclear Proteins; D051996:Transcription Factor RelA; C499072:Gadd45a protein, mouse; D000097642:GADD45 Proteins