title: Residue specific hydration of primary cell wall potato pectin identified by solid-state 13C single-pulse MAS and CP/MAS NMR spectroscopy.
abstract: Hydration of rhamnogalacturonan-I (RG-I) derived from potato cell wall was analyzed by (13)C single-pulse (SP) magic-angle-spinning (MAS) and (13)C cross-polarization (CP) MAS nuclear magnetic resonance (NMR) and supported by (2)H SP/MAS NMR experiments. The study shows that the arabinan side chains hydrate more readily than the galactan side chains and suggests that the overall hydration properties can be controlled by modifying the ratio of these side chains. Enzymatic modification of native (NA) RG-I provided samples with reduced content of arabinan (sample DA), galactan (sample DG), or both side chains (sample DB). Results of these samples suggested that hydration properties were determined by the length and character of the side chains. NA and DA exhibited similar hydration characteristics, whereas DG and DB were difficult to hydrate because of the less hydrophilic properties of the rhamnose-galacturonic acid (Rha-GalA) backbone in RG-I. Potential food ingredient uses of RG-I by tailoring of its structure are discussed.
authors.affiliation: Department of Food Science, University of Copenhagen, Frederiksberg C, Denmark. fhl@life.ku.dk
authors.forename: Flemming H
authors.initials: FH
authors.lastname: Larsen
authors.forename: Inge
authors.initials: I
authors.lastname: Byg
authors.forename: Iben
authors.initials: I
authors.lastname: Damager
authors.forename: Jerome
authors.initials: J
authors.lastname: Diaz
authors.forename: Søren B
authors.initials: SB
authors.lastname: Engelsen
authors.forename: Peter
authors.initials: P
authors.lastname: Ulvskov
mesh_terms: D002247:Carbon Isotopes; D002473:Cell Wall / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D010368:Pectins / Q000737:chemistry*; D011198:Solanum tuberosum / Q000737:chemistry*; D014867:Water / Q000737:chemistry*
chemical_list: D002247:Carbon Isotopes; D014867:Water; D010368:Pectins