title: Identification of positional candidates for bovine placental genes responsible for early embryonic death during cloning-attempted pregnancy.
abstract: Our previous study detected 291 and 77 genes showing early embryonic death-associated elevation and reduction of expression, respectively, in the fetal placenta of the cow carrying somatic nuclear transfer-derived cloned embryo. In this study, we mapped the 10 genes showing the elevation and the 10 genes doing the reduction most significantly, using somatic cell hybrid and bovine draft genome sequence. We then compared the mapped positions for these genes with the genomic locations of bovine quantitative trait loci for still-birth and/or abortion. Among the mapped genes, peptidylglycine alpha-amidating monooxygenase (PAM), spectrin, beta, nonerythrocytic 1 (SPTBNI), and an unknown novel gene containing AU277832 expressed sequence tag were intriguing, in that the mapped positions were consistent with the genomic locations of bovine still-birth and/or abortion quantitative trait loci, and thus identified as positional candidates for bovine placental genes responsible for the early embryonic death during the pregnancy attempted by somatic nuclear transfer-derived cloning.
authors.affiliation: Laboratory of Animal Breeding and Genetics, Graduate School ofAgriculture, Kyoto University, Sakyoku, Kyoto 606-8502, Japan. tyamada@kais.kyoto-u.ac.jp
authors.forename: Takahisa
authors.initials: T
authors.lastname: Yamada
authors.forename: Youji
authors.initials: Y
authors.lastname: Muramatsu
authors.forename: Yukio
authors.initials: Y
authors.lastname: Taniguchi
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Sasaki
mesh_terms: D000818:Animals; D002417:Cattle; D002874:Chromosome Mapping; D019976:Cloning, Organism / Q000662:veterinary*; D020964:Embryo Loss / Q000235:genetics / Q000662:veterinary*; D020224:Expressed Sequence Tags; D005260:Female; D015870:Gene Expression; D006899:Mixed Function Oxygenases / Q000235:genetics; D009097:Multienzyme Complexes / Q000235:genetics; D053652:Nuclear Transfer Techniques / Q000662:veterinary; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000235:genetics*; D040641:Quantitative Trait Loci; D013049:Spectrin / Q000235:genetics
chemical_list: D009097:Multienzyme Complexes; D013049:Spectrin; D006899:Mixed Function Oxygenases; C035189:peptidylglycine monooxygenase