title: Biodegradation of carbazole by the seven Pseudomonas sp. strains and their denitrification potential.
abstract: Carbazole, one representative of non-alkaline nitrogen heterocyclic compounds, is widespread in the natural environment and harmful to human health. In this research, the seven bacterial strains using carbazole as their sole carbon, nitrogen and energy source were isolated from activated sludge of a coking wastewater treatment plant. All strains efficiently degraded 500 mg/L of carbazole in the medium within 36 h. Based on the DNA sequence and phylogenetic tree analysis, the seven strains were identified as the genera Pseudomonas with different evolutionary pathways. PCR analysis revealed that the seven isolates carried the car gene. Moreover, all of these strains could utilize and transform ammonium and nitrate efficiently, and the six strains except BC043 strain coded the nitrite reductase gene (nirS) and the nitrous oxide reductase (nosZ), that indicated their denitrification ability. All these strains may be useful in the bioremediation of environments contaminated by carbazole.
authors.affiliation: College of Environmental Sciences and Engineering, The Key Laboratory of Water and Sediment Sciences (Ministry of Education), Peking University, Beijing 100871, People's Republic of China.
authors.forename: Cui
authors.initials: C
authors.lastname: Zhao
authors.forename: Yin
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xiaobao
authors.initials: X
authors.lastname: Li
authors.forename: Donghui
authors.initials: D
authors.lastname: Wen
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Tang
mesh_terms: D001483:Base Sequence; D001673:Biodegradation, Environmental; D002227:Carbazoles / Q000378:metabolism*; D002244:Carbon; D002273:Carcinogens; D058440:Denitrification; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000378:metabolism; D010802:Phylogeny; D011549:Pseudomonas / Q000235:genetics / Q000378:metabolism*; D012722:Sewage / Q000382:microbiology
chemical_list: D002227:Carbazoles; D002273:Carcinogens; D012722:Sewage; C041514:carbazole; D002244:Carbon; D009584:Nitrogen