title: Influence of pulsed magnetic fields on the morphology of bone cells in early stages of growth.
abstract: The effect of electromagnetic fields on living systems has been studied both in vivo and in vitro in a wide range of organisms, cells and tissues. However, the mechanism of action of electromagnetic fields is not yet clearly defined. This paper presents the results of applying a pulsed magnetic field of 70ms width, intensity of 0.65mT at 4Hz in human osteoblasts, during 45min. The magnetic field application was conducted on crops of both 24 and 48h of proliferation. The effect of applying magnetic fields was assessed using parameters such as cell density, protein content, distribution of F-actin fibrils and β-tubulin and integrity of nuclear structure. The results indicate no alteration in either protein synthesis or nuclear structure, or in the number of cells. However, we observed that exposure to these fields induces changes in the distribution of cytoskeletal proteins of osteoblasts.
authors.affiliation: Department of Chemistry, University of Guanajuato, 36050 Guanajuato, Gto., Mexico.
authors.forename: Berenice
authors.initials: B
authors.lastname: Noriega-Luna
authors.forename: Myrna
authors.initials: M
authors.lastname: Sabanero
authors.forename: Modesto
authors.initials: M
authors.lastname: Sosa
authors.forename: Mario
authors.initials: M
authors.lastname: Avila-Rodriguez
mesh_terms: D008841:Actin Cytoskeleton / Q000528:radiation effects; D000199:Actins / Q000528:radiation effects; D001842:Bone and Bones / Q000166:cytology / Q000528:radiation effects*; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000528:radiation effects; D048430:Cell Shape; D003598:Cytoskeletal Proteins; D004574:Electromagnetic Fields; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008280:Magnetics; D008855:Microscopy, Electron, Scanning; D008870:Microtubules / Q000528:radiation effects; D010006:Osteoblasts / Q000166:cytology / Q000528:radiation effects*; D014176:Protein Biosynthesis / Q000528:radiation effects
chemical_list: D000199:Actins; D003598:Cytoskeletal Proteins