title: Determination of corosolic acid, a natural potential anti-diabetes compound, in rat plasma by high-performance liquid chromatography-mass spectrometry and its application to pharmacokinetic and bioavailability studies.
abstract: A simple, robust, and sensitive high-performance liquid chromatography/mass spectrometric method was developed for the determination of corosolic acid, a potential anti-diabetes substance, in rat plasma using glycyrrhetinic acid as the internal standard (IS). This method involved a liquid-liquid extraction with acetic ether and a subsequent analysis performed on an LC-MS system which contained an electrospray ionization interface. Chromatographic separation was performed using an ODS column, and the mobile phase was composed of methanol and 5 mmol/L ammonium acetate (88 : 12, v/v). Good linearity was observed over the concentration range of 20-10 ,000 ng/mL with a correlation coefficient (r² ≥ 0.995. The method was proved to be accurate and reliable and was applied to a pharmacokinetic study in the rat following intragastric and intravenous administration of corosolic acid.
authors.affiliation: College of Pharmaceutical Sciences, Southwest University, Chongqing, P. R. China.
authors.forename: Qingwang
authors.initials: Q
authors.lastname: Liu
authors.forename: Di
authors.initials: D
authors.lastname: Zhao
authors.forename: Xijing
authors.initials: X
authors.lastname: Chen
authors.forename: Zhubo
authors.initials: Z
authors.lastname: Li
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.forename: Deen
authors.initials: D
authors.lastname: Han
authors.forename: Amer
authors.initials: A
authors.lastname: Talbi
authors.forename: Tarcisse
authors.initials: T
authors.lastname: Biwoele
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001682:Biological Availability; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards; D004355:Drug Stability; D006034:Glycyrrhetinic Acid / Q000032:analysis; D007004:Hypoglycemic Agents / Q000097:blood* / Q000493:pharmacokinetics*; D007275:Injections, Intravenous; D059625:Liquid-Liquid Extraction; D010949:Plasma; D011786:Quality Control; D051381:Rats; D017207:Rats, Sprague-Dawley; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization; D013997:Time Factors; D014315:Triterpenes / Q000097:blood* / Q000493:pharmacokinetics*
chemical_list: D007004:Hypoglycemic Agents; D014315:Triterpenes; C113861:corosolic acid; D006034:Glycyrrhetinic Acid