title: Plasma proteomic analysis of the critical limb ischemia markers in diabetic patients with hemodialysis.
abstract: Critical limb ischemia (CLI) is a severe obstruction of the arteries resulting from seriously decreased blood flow to the extremities, progressing to the point of pain and even skin ulcers or sores. CLI is associated with a high percentage of limb loss and mortality; however, no reliable biochemical indices are available to monitor the stages of CLI. We developed a strategy involving comparative proteomic analysis to detect CLI associated plasma biomarkers. 2D-DIGE and subsequent MALDI-TOF MS analyses provided 50 differentially expressed plasma proteins (including alkaline phosphatase and haptoglobin), between hemodialytic diabetic patients with and without CLI. Interestingly, more than half of the differentially expressed plasma proteins are associated with inflammatory responses. Our results show that CLI is strongly correlated to inflammation, indicating a strong potential for proteomics analysis in the diagnosis of CLI. To the best of our knowledge, this is the first report presenting a proteomics approach to monitor differentially expressed plasma proteins associated with CLI.
authors.affiliation: Division of Nephrology, Department of Internal Medicine, Chia-yi Christian Hospital, Chia-yi, Taiwan.
authors.forename: Peir-Haur
authors.initials: PH
authors.lastname: Hung
authors.forename: Yi-Wen
authors.initials: YW
authors.lastname: Chen
authors.forename: Kuang-Chi
authors.initials: KC
authors.lastname: Cheng
authors.forename: Hsiu-Chuan
authors.initials: HC
authors.lastname: Chou
authors.forename: Ping-Chiang
authors.initials: PC
authors.lastname: Lyu
authors.forename: Ying-Chieh
authors.initials: YC
authors.lastname: Lu
authors.forename: Ying-Ray
authors.initials: YR
authors.lastname: Lee
authors.forename: Cheng-Tao
authors.initials: CT
authors.lastname: Wu
authors.forename: Hong-Lin
authors.initials: HL
authors.lastname: Chan
mesh_terms: D000368:Aged; D051017:Apoptosis Regulatory Proteins; D001132:Arm / Q000098:blood supply* / Q000473:pathology; D015415:Biomarkers / Q000097:blood; D001798:Blood Proteins / Q000378:metabolism*; D016022:Case-Control Studies; D048909:Diabetes Complications / Q000097:blood* / Q000473:pathology / Q000628:therapy; D006242:Haptoglobins / Q000378:metabolism; D006801:Humans; D007511:Ischemia / Q000097:blood* / Q000209:etiology; D007866:Leg / Q000098:blood supply* / Q000473:pathology; D008875:Middle Aged; D010949:Plasma; D020543:Proteome / Q000378:metabolism*; D051116:Receptors, Scavenger; D006435:Renal Dialysis; D051122:Scavenger Receptors, Class B / Q000097:blood; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D057825:Two-Dimensional Difference Gel Electrophoresis
chemical_list: D051017:Apoptosis Regulatory Proteins; D015415:Biomarkers; D001798:Blood Proteins; C492821:CD5L protein, human; C485359:HP protein, human; D006242:Haptoglobins; D020543:Proteome; D051116:Receptors, Scavenger; D051122:Scavenger Receptors, Class B