title: Phylogenetic analysis of C, M, N, and U genomes and their relationships with Triticum and other related genomes as revealed by LMW-GS genes at Glu-3 loci.
abstract: Phylogenetic relationships between the C, U, N, and M genomes of Aegilops species and the genomes of common wheat and other related species were investigated by using three types of low-molecular-weight glutenin subunit (LMW-GS) genes at Glu-3 loci. A total of 20 LMW-GS genes from Aegilops and Triticum species were isolated, including 11 LMW-m type and 9 LMW-i type genes. Particularly, four LMW-m type and three LMW-i type subunits encoded by the genes on the C, N, and U genomes possessed an extra cysteine residue at conserved positions, which could provide useful information for understanding phylogenetic relationships among Aegilops and Triticum genomes. Phylogenetic trees constructed by using either LMW-i or the combination of LMW-m and LMW-s, as well as analysis of all the three types of LMW-GS genes together, demonstrated that the C and U genomes were closely related to the A genome, whereas the N and M genomes were closely related to the D genome. Our results support previous findings that the A genome was derived from Triticum uratu, the B genome was from Aegilops speltoides, and the D genome was from Aegilops tauschii. In addition, phylogenetic relationships among different genomes analysed in this study support the concept that Aegilops is not monophyletic.
authors.affiliation: Key Laboratory of Genetics and Biotechnology, College of Life Science, Capital Normal University, 100048 Beijing, China.
authors.forename: Shunli
authors.initials: S
authors.lastname: Wang
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Li
authors.forename: Ke
authors.initials: K
authors.lastname: Wang
authors.forename: Xiaozheng
authors.initials: X
authors.lastname: Wang
authors.forename: Shanshan
authors.initials: S
authors.lastname: Li
authors.forename: Yanzhen
authors.initials: Y
authors.lastname: Zhang
authors.forename: Guangfang
authors.initials: G
authors.lastname: Guo
authors.forename: Friedrich J
authors.initials: FJ
authors.lastname: Zeller
authors.forename: Sai L K
authors.initials: SL
authors.lastname: Hsam
authors.forename: Yueming
authors.initials: Y
authors.lastname: Yan
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D019143:Evolution, Molecular; D018745:Genome, Plant / Q000235:genetics*; D005983:Glutens / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010802:Phylogeny; D006109:Poaceae / Q000145:classification / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014908:Triticum / Q000145:classification / Q000235:genetics*
chemical_list: D018744:DNA, Plant; D005983:Glutens; C037302:glutenin