title: Development of a reversed phase high performance liquid chromatography method based on the use of cyclodextrins as mobile phase additives to determine pterostilbene in blueberries.
abstract: In this work, a reversed phase high performance liquid chromatography (RP-HPLC) method was developed for the determination of pterostilbene in food samples. The novel method is based on the addition of cyclodextrins (CDs) to the mobile phase where the complexation of pterostilbene by CDs is carried out. In order to select the most suitable conditions for the RP-HPLC method, the effect of several physico-chemical parameters on the complexation of pterostilbene by CDs was studied. Our results show that the addition of 12 mM HP-β-CD to a 50:50 (v/v) methanol:water mobile phase at 25°C and pH 7.0 significantly improves the main analytical parameters. In addition, it was seen that pterostilbene forms a 1:1 complex with HP-β-CD, showing an apparent complexation constant of 251±13 M(-1). Finally, in order to study the validity of the proposed method, blueberries were analyzed and the concentration of pterostilbene has been determined.
authors.affiliation: Department of Biochemistry and Molecular Biology-A, Faculty of Biology, University of Murcia, Murcia, Spain.
authors.forename: Pilar
authors.initials: P
authors.lastname: Rodríguez-Bonilla
authors.forename: José Manuel
authors.initials: JM
authors.lastname: López-Nicolás
authors.forename: Lorena
authors.initials: L
authors.lastname: Méndez-Cazorla
authors.forename: Francisco
authors.initials: F
authors.lastname: García-Carmona
mesh_terms: D000073738:2-Hydroxypropyl-beta-cyclodextrin; D029798:Blueberry Plants / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D016014:Linear Models; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013267:Stilbenes / Q000032:analysis* / Q000737:chemistry; D013696:Temperature; D014867:Water; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D013267:Stilbenes; D047392:beta-Cyclodextrins; D014867:Water; D000073738:2-Hydroxypropyl-beta-cyclodextrin; C107773:pterostilbene