title: SMETHILLIUM: spatial normalization method for Illumina infinium HumanMethylation BeadChip.
abstract: CONCLUSIONS
  DNA methylation is a major epigenetic modification in human cells. Illumina HumanMethylation27 BeadChip makes it possible to quantify the methylation state of 27 578 loci spanning 14 495 genes. We developed a non-parametric normalization method to correct the spatial background noise in order to improve the signal-to-noise ratio. The prediction performance of the proposed method was assessed on three fully methylated samples and three fully unmethylated DNA samples. We demonstrate that the spatial normalization outperforms BeadStudio to predict the methylation state of a given locus.
  
  
  METHODS
  A R script and the data are available at the following address: http://bioinfo.curie.fr/projects/smethillium.
authors.affiliation: Institut Curie, 26 rue d'Ulm, F-75248, Paris, France.
authors.forename: Camille
authors.initials: C
authors.lastname: Sabbah
authors.forename: Gildas
authors.initials: G
authors.lastname: Mazo
authors.forename: Caroline
authors.initials: C
authors.lastname: Paccard
authors.forename: Fabien
authors.initials: F
authors.lastname: Reyal
authors.forename: Philippe
authors.initials: P
authors.lastname: Hupé
mesh_terms: D019175:DNA Methylation; D044127:Epigenesis, Genetic; D006801:Humans; D015335:Molecular Probes; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012984:Software
chemical_list: D015335:Molecular Probes