title: Cigarette smoke extract induced rat pulmonary artery smooth muscle cells proliferation via PKCα-mediated cyclin D1 expression.
abstract: Cigarette smoke could induce pulmonary smooth muscle cells (PASMCs) proliferation. Although our previous study had implied the involvement of protein kinase Cα (PKCα), the molecular mechanism underlying PKCα pathway in this process is still unknown. In this study, rat PASMCs were stimulated by cigarette smoke extract (CSE) or PMA (a special activator to PKCα). Two percent CSE and PMA significantly enhanced cyclin D1 expression and cells proliferation. But cyclin D1-specific siRNA successfully inhibited DNA synthesis in CSE-treated or PMA-treated cells. On the other hand, PKCα-specific siRNA significantly suppressed cyclin D1 expression in CSE-treated cells. Moreover, PKCα-specific siRNA resulted in a cell-cycle arrest in G0/G1 and decreased cells number significantly. We conclude that CSE induced rat PASMCs proliferation at least partly via PKCα-mediated cyclin D1 expression.
authors.affiliation: Key Laboratory of Pulmonary Diseases of Ministry of Health, Department of Respiratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, PR China.
authors.forename: Da-Xiong
authors.initials: DX
authors.lastname: Zeng
authors.forename: Yong-Jian
authors.initials: YJ
authors.lastname: Xu
authors.forename: Xian-Sheng
authors.initials: XS
authors.lastname: Liu
authors.forename: Ran
authors.initials: R
authors.lastname: Wang
authors.forename: Min
authors.initials: M
authors.lastname: Xiang
mesh_terms: D000818:Animals; D002273:Carcinogens / Q000494:pharmacology; D002478:Cells, Cultured; D045424:Complex Mixtures / Q000009:adverse effects / Q000494:pharmacology*; D019938:Cyclin D1 / Q000096:biosynthesis*; D020536:Enzyme Activators / Q000494:pharmacology; D016193:G1 Phase / Q000187:drug effects*; D005786:Gene Expression Regulation / Q000187:drug effects*; D009131:Muscle, Smooth, Vascular / Q000378:metabolism* / Q000473:pathology; D032389:Myocytes, Smooth Muscle / Q000378:metabolism* / Q000473:pathology; D051571:Protein Kinase C-alpha / Q000378:metabolism*; D011651:Pulmonary Artery / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D016192:Resting Phase, Cell Cycle / Q000187:drug effects*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014028:Tobacco Smoke Pollution
chemical_list: D002273:Carcinogens; C530106:Ccnd1 protein, rat; D045424:Complex Mixtures; D020536:Enzyme Activators; D014028:Tobacco Smoke Pollution; D019938:Cyclin D1; D051571:Protein Kinase C-alpha; D013755:Tetradecanoylphorbol Acetate