title: Tunable reagents for multi-functional bioconjugation: reversible or permanent chemical modification of proteins and peptides by control of maleimide hydrolysis.
abstract: Controlling maleimide hydrolysis allows the modular construction of bromomaleimide-mediated bioconjugates which are either stable or cleavable in an aqueous, thiol-mediated reducing environment. The application of this methodology to reversible protein biotinylation, the irreversible labeling of peptide disulfide bonds and the assembly of stable, fluorescein-labelled glycoprotein mimics is described.
authors.affiliation: Department of Chemistry, University College London, 20 Gordon Street, London WC1H 0AJ, UK.
authors.forename: Chris P
authors.initials: CP
authors.lastname: Ryan
authors.forename: Mark E B
authors.initials: ME
authors.lastname: Smith
authors.forename: Felix F
authors.initials: FF
authors.lastname: Schumacher
authors.forename: Dina
authors.initials: D
authors.lastname: Grohmann
authors.forename: Danai
authors.initials: D
authors.lastname: Papaioannou
authors.forename: Gabriel
authors.initials: G
authors.lastname: Waksman
authors.forename: Finn
authors.initials: F
authors.lastname: Werner
authors.forename: James R
authors.initials: JR
authors.lastname: Baker
authors.forename: Stephen
authors.initials: S
authors.lastname: Caddick
mesh_terms: D000595:Amino Acid Sequence; D040761:Biomimetic Materials / Q000737:chemistry; D019921:Biotinylation; D006023:Glycoproteins / Q000737:chemistry; D006868:Hydrolysis; D007202:Indicators and Reagents / Q000737:chemistry; D008301:Maleimides / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D055550:Protein Stability; D011506:Proteins / Q000737:chemistry*
chemical_list: D006023:Glycoproteins; D007202:Indicators and Reagents; D008301:Maleimides; D010455:Peptides; D011506:Proteins; C043592:maleimide