title: Efficient double-strand cleavage of DNA mediated by Zn(II)-based artificial nucleases.
abstract: Two water-soluble zinc complexes, [Zn(L)Cl(2)] (1) and [Zn(2)(L)(2)(μ-C(2)O(4))(H(2)O)(2)]·(ClO(4))(2)·CH(3)OH (2) (L = N,N-bis(2-pyridylmethyl)methylamine), were prepared to serve as nuclease mimics. The complexes were characterized by X-ray, IR and UV-vis spectroscopy as well as ESI-MS. The electrospray mass spectrum of 2 in solution indicates that dinuclear ion [Zn(2)(L)(2)(μ-C(2)O(4))(ClO(4))](+) (3) is the active species. UV-Vis absorption and fluorescence spectroscopy studies show that the complexes partially intercalate to CT-DNA. In the absence of reducing agent, supercoiled plasmid DNA cleavage by the complexes 1 and 3 was performed and the hydrolytic mechanism was demonstrated by adding standard radical scavengers.
authors.affiliation: College of Chemistry, Tianjin Normal University, Tianjin, 300387, P. R. China. qianjinger@yahoo.com.cn
authors.forename: Jing
authors.initials: J
authors.lastname: Qian
authors.forename: Liping
authors.initials: L
authors.lastname: Wang
authors.forename: Wen
authors.initials: W
authors.lastname: Gu
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.forename: Jinlei
authors.initials: J
authors.lastname: Tian
authors.forename: Shiping
authors.initials: S
authors.lastname: Yan
mesh_terms: D040761:Biomimetic Materials / Q000737:chemistry / Q000378:metabolism; D056831:Coordination Complexes / Q000737:chemistry*; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D053903:DNA Breaks, Double-Stranded; D003851:Deoxyribonucleases / Q000737:chemistry* / Q000378:metabolism; D008968:Molecular Conformation; D013056:Spectrophotometry, Ultraviolet; D015032:Zinc / Q000737:chemistry*
chemical_list: D056831:Coordination Complexes; D004247:DNA; D003851:Deoxyribonucleases; D015032:Zinc