title: Imaging mass spectrometry reveals characteristic changes in triglyceride and phospholipid species in regenerating mouse liver.
abstract: After partial hepatectomy (PH), regenerating liver accumulates unknown lipid species. Here, we analyzed lipids in murine liver and adipose tissues following PH by thin-layer chromatography (TLC), imaging mass spectrometry (IMS), and real-time RT-PCR. In liver, IMS revealed that a single TLC band comprised major 19 TG species. Similarly, IMS showed a single phospholipid TLC band to be major 13 species. In adipose tissues, PH induced changes to expression of genes regulating lipid metabolism. Finally, IMS of phosphatidylcholine species demonstrated distribution gradients in lobules that resembled hepatic zonation. IMS is thus a novel and power tool for analyzing lipid species with high resolution.
authors.affiliation: Dept. Developmental and Regenerative Biology, Medical Research Institute, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: Norio
authors.initials: N
authors.lastname: Miyamura
authors.forename: Takashi
authors.initials: T
authors.lastname: Nakamura
authors.forename: Naoko
authors.initials: N
authors.lastname: Goto-Inoue
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Zaima
authors.forename: Takahiro
authors.initials: T
authors.lastname: Hayasaka
authors.forename: Tokiwa
authors.initials: T
authors.lastname: Yamasaki
authors.forename: Shuji
authors.initials: S
authors.lastname: Terai
authors.forename: Isao
authors.initials: I
authors.lastname: Sakaida
authors.forename: Mitsutoshi
authors.initials: M
authors.lastname: Setou
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nishina
mesh_terms: D000818:Animals; D002855:Chromatography, Thin Layer; D005260:Female; D008099:Liver / Q000737:chemistry* / Q000378:metabolism; D008115:Liver Regeneration; D051379:Mice; D008810:Mice, Inbred C57BL; D010743:Phospholipids / Q000032:analysis* / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D014280:Triglycerides / Q000032:analysis* / Q000378:metabolism
chemical_list: D010743:Phospholipids; D014280:Triglycerides