title: A glucose sensor fabricated by piezoelectric inkjet printing of conducting polymers and bienzymes.
abstract: Piezoelectric inkjet printing of polymers and proteins holds great promise for fabrication of miniaturized bioelectronic devices, such as biochips and biosensors. In this study, a bienzymatic glucose biosensor prototype based on poly(3,4-ethylenedioxythiophene)-poly(styrene sulfonic acid) (PEDOT-PSS), glucose oxidase (GOD), and horseradish peroxidase (HRP) was fabricated by a piezoelectric inkjet printer. An aqueous bioelectrical ink containing PEDOT-PSS, GOD, and HRP was prepared and printed on an indium-tin-oxide (ITO)-coated poly(ethylene terephthalate) (PET) film. The PEDOT-PSS/GOD/HRP sensor was covered with a cellulose acetate membrane. The use of bienzymatic sensing combined with conducting polymers via piezoelectric inkjet printing showed a synergistic effect resulting in significant amplification of the response signal. The glucose sensor reached steady-state current density within 3 s, indicating a fast response time, and exhibited a linear dose-dependent electrochemical response with high sensitivity. The overall result demonstrates that a glucose sensor with high sensitivity could be readily fabricated by a piezoelectric inkjet printing system.
authors.affiliation: Department of Chemical Engineering, Hanyang University, Ansan, Gyeonggi-do, Republic of Korea.
authors.forename: Yeon Hee
authors.initials: YH
authors.lastname: Yun
authors.forename: Byung Kook
authors.initials: BK
authors.lastname: Lee
authors.forename: Ji Suk
authors.initials: JS
authors.lastname: Choi
authors.forename: Sungwon
authors.initials: S
authors.lastname: Kim
authors.forename: Bongyoung
authors.initials: B
authors.lastname: Yoo
authors.forename: Yong Shin
authors.initials: YS
authors.lastname: Kim
authors.forename: Kinam
authors.initials: K
authors.lastname: Park
authors.forename: Yong Woo
authors.initials: YW
authors.lastname: Cho
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004553:Electric Conductivity; D004563:Electrochemistry; D005947:Glucose / Q000032:analysis*; D005948:Glucose Dehydrogenases / Q000378:metabolism*; D006735:Horseradish Peroxidase / Q000378:metabolism*; D007281:Ink; D011137:Polystyrenes / Q000737:chemistry*; D011327:Printing / Q000379:methods*; D013876:Thiophenes / Q000737:chemistry*; D017971:Tin Compounds / Q000737:chemistry
chemical_list: D011137:Polystyrenes; D013876:Thiophenes; D017971:Tin Compounds; C533756:poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate); C109984:indium tin oxide; D005948:Glucose Dehydrogenases; D006735:Horseradish Peroxidase; D005947:Glucose