title: Stimulated production of triterpenoids of Ganoderma lucidum by an ether extract from the medicinal insect, Catharsius molossus, and identification of the key stimulating active components.
abstract: The medicinal fungus Ganoderma lucidum was inoculated into the media with and without supplementation of medicinal insect extracts to screen stimulators from Chinese medicinal insects for mycelial growth and triterpenoids production in submerged fermentation. The methanol and ether extracts of the tested insects had no significant stimulatory effect on the mycelial biomass production (P > 0.05), and those of H. remigator and Mylabris phalerata markedly inhibited the mycelial growth. However, the ether extract of Catharsius molossus at a concentration of 200 mg l(-1) led to a significant increase in triterpenoids concentration from 231.7 ± 9.77 to 313.7 ± 10.6 mg l(-1) (P < 0.01). Analysis of fermentation kinetics of G. lucidum suggests that glucose concentration in the extract of C. molossus-added group decreased more quickly as compared to the control group from day 2 to day 7 of fermentation process, while the triterpenoids biosynthesis was promoted at the same culture period. However, the culture pH profile was not affected by the addition of the extract. Chemical study of the extract show that cis-9,10-methylenehexadecanoic acid (9,10-MEA) and hexadecanoic acid (especially 9,10-MEA) were the key active compounds of the extract responsible for the stimulatory effect on the triterpenoids production.
authors.affiliation: Hunan Provincial Key Laboratory of Forestry Biotechnology, College of Life Science and Technology, Central South University of Forestry and Technology, 498 South Shaoshan Road, 410004, Changsha, China. gaoliuedu@yahoo.com.cn
authors.forename: Gao-Qiang
authors.initials: GQ
authors.lastname: Liu
authors.forename: Hua-Xi
authors.initials: HX
authors.lastname: Xiao
authors.forename: Xiao-Ling
authors.initials: XL
authors.lastname: Wang
authors.forename: Yao
authors.initials: Y
authors.lastname: Zhao
authors.forename: Yong-Guang
authors.initials: YG
authors.lastname: Zhang
authors.forename: Guo-Pu
authors.initials: GP
authors.lastname: Ren
mesh_terms: D000818:Animals; D001517:Coleoptera / Q000737:chemistry*; D004986:Ether / Q000737:chemistry*; D020940:Reishi / Q000187:drug effects* / Q000378:metabolism*; D014315:Triterpenes / Q000378:metabolism*
chemical_list: D014315:Triterpenes; D004986:Ether