title: Quantitation of cellular components in Cryptococcus neoformans for system biology analysis.
abstract: Methods and procedures in molecular biology used to study fungal pathogenesis have significantly improved during the last decade. In this chapter, we provide step-by-step procedures for performing genetics and biochemical studies in the human pathogenic fungal microorganism Cryptococcus neoformans (Cn). These methods are employed for studying the pathobiology of Cn and for experimental validation of theoretical models of fungal pathogenicity.
authors.affiliation: Department of Biochemistry, Medical University of South Carolina, Charleston, SC, USA.
authors.forename: Arpita
authors.initials: A
authors.lastname: Singh
authors.forename: Asfia
authors.initials: A
authors.lastname: Qureshi
authors.forename: Maurizio
authors.initials: M
authors.lastname: Del Poeta
mesh_terms: D019470:Biolistics; D015139:Blotting, Southern; D002855:Chromatography, Thin Layer; D003455:Cryptococcus neoformans / Q000166:cytology* / Q000378:metabolism; D004271:DNA, Fungal / Q000302:isolation & purification; D057075:Enzyme Assays; D005656:Fungal Proteins / Q000302:isolation & purification; D006801:Humans; D006868:Hydrolysis; D008055:Lipids / Q000737:chemistry / Q000302:isolation & purification; D013058:Mass Spectrometry; D012331:RNA, Fungal / Q000302:isolation & purification; D049490:Systems Biology / Q000379:methods*
chemical_list: D004271:DNA, Fungal; D005656:Fungal Proteins; D008055:Lipids; D012331:RNA, Fungal