title: CD117⁺ CD3⁻ CD56⁻ OX40Lhigh cells express IL-22 and display an LTi phenotype in human secondary lymphoid tissues.
abstract: Here, we identify cells within human adult secondary lymphoid tissues that are comparable in phenotype and location to the lymphoid tissue inducer (LTi) cells that persist in the adult mouse. Identified as CD117(+) CD3(-) CD56(-) cells, like murine LTi cells, they lack expression of many common lineage markers and express CD127, OX40L and TRANCE. These cells were detected at the interface between the B- and T- zones, as well as at the subcapsular sinus in LNs, the location where LTi cells reside in murine spleen and LNs. Furthermore, like murine LTi cells, these cells expressed high levels of IL-22 and upregulated IL-22 expression upon IL-23 stimulation. Importantly, these cells were not an NK cell subset since they showed no expression of IFN-γ and perforin. Interestingly, a subset of the CD117(+) CD3(-) CD56(-) OX40L(+) population expressed NKp46, again similar to recent findings in mice. Finally, these cells supported memory CD4(+) T-cell survival in an OX40L-dependent manner. Combined, these data indicate that the CD117(+) CD3(-) CD56(-) OX40L(+) cells in human secondary lymphoid tissues are comparable in phenotype, location and function to the LTi cells that persist within adult murine secondary lymphoid tissues.
authors.affiliation: Department of Bioinformatics and Life Science, Soongsil University, Seoul, Korea.
authors.forename: Soochan
authors.initials: S
authors.lastname: Kim
authors.forename: Sinsuk
authors.initials: S
authors.lastname: Han
authors.forename: David R
authors.initials: DR
authors.lastname: Withers
authors.forename: Fabrina
authors.initials: F
authors.lastname: Gaspal
authors.forename: Jingyu
authors.initials: J
authors.lastname: Bae
authors.forename: Song
authors.initials: S
authors.lastname: Baik
authors.forename: Hyun-Chool
authors.initials: HC
authors.lastname: Shin
authors.forename: Kyung-Su
authors.initials: KS
authors.lastname: Kim
authors.forename: Vasileios
authors.initials: V
authors.lastname: Bekiaris
authors.forename: Graham
authors.initials: G
authors.lastname: Anderson
authors.forename: Peter
authors.initials: P
authors.lastname: Lane
authors.forename: Mi-Yeon
authors.initials: MY
authors.lastname: Kim
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D002454:Cell Differentiation; D002469:Cell Separation; D002470:Cell Survival; D002478:Cells, Cultured; D005434:Flow Cytometry; D006801:Humans; D053759:Interleukin-23 / Q000276:immunology / Q000378:metabolism; D007378:Interleukins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D008198:Lymph Nodes / Q000166:cytology; D051379:Mice; D055627:Natural Cytotoxicity Triggering Receptor 1 / Q000096:biosynthesis; D053278:OX40 Ligand / Q000276:immunology / Q000378:metabolism*; D014066:Palatine Tonsil / Q000166:cytology*; D058504:Th17 Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D000097043:Interleukin-22
chemical_list: D015703:Antigens, CD; D053759:Interleukin-23; D007378:Interleukins; D055627:Natural Cytotoxicity Triggering Receptor 1; D053278:OX40 Ligand; D000097043:Interleukin-22; C112260:NCR1 protein, human