title: [Effects of IL-13 and alcohol on collagen expression of human lung fibrolasts].
abstract: OBJECTIVE
  To investigate the effect of alcohol and/or IL-13 on the expression of collagen type I alpha 1 chain gene (COL1A1, COL3A1) mRNA and collagen production of human lung fibroblast(HFL-1).
  
  
  METHODS
  HFL-1 was cultured and real time RT-PCR was used to determine the expression of IL-13 receptor (IL-13Rα1, IL-13Rα2, IL-4Rα) mRNA, COL1A1 mRNA and COL3A1 mRNA. ELISA was used to determine the production of collagen type I.
  
  
  RESULTS
  Low level of Alcohol (25, 50, 100, 200 mmol/L) greatly up-regulated the expression of IL-13Rα1 mRNA and IL-4Rα mRNA of HFL-1, but IL-13Rα2 mRNA expression was decreased. low level of alcohol (25, 50, 100, 200 mmol/L) had no effect on the expression of COL1A1 and COL3A1. IL-13 (10, 20, 50 μg/L) stimulated the expression of COL1A1 and COL3A1 in a dose-dependent manner. The expression of COL1A1 and COL3A1 induced by combination of alcohol (200 mmol/L) and IL-13(10 μg/L, 20 μg/L, 50 μg/L) was significantly higher than IL-13(10, 20, 50 μg/L) alone. Combination of alcohol (200 mmol/L) and IL-13(10, 20, 50 μg/L) could significantly increase collagen typeI production than IL-13(10, 20, 50 μg/L) alone.
  
  
  CONCLUSIONS
  Low level of alcohol has no effect on the mRNA expression of COL1A1 and COL3A1 of HFL-1cells, but has great effect on the mRNA expression of IL-13Rα1, IL-13Rα2 and IL-4Rα. Combination of alcohol and IL-13 significantly up-regulated the collagen production and expression of COL1A1 and COL3A1 of HFL-1 cells.
authors.affiliation: Medical College of Nanchang University Jiangxi Institute of Medical Research, Key Laboratory of Medical Biotechnology, Nanchang 330006, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xiong
authors.forename: Wu
authors.initials: W
authors.lastname: Sun
authors.forename: Wen-lin
authors.initials: WL
authors.lastname: Li
authors.forename: Li-xia
authors.initials: LX
authors.lastname: Xiong
authors.forename: Xiao-yu
authors.initials: XY
authors.lastname: Shi
authors.forename: Lin
authors.initials: L
authors.lastname: Zhao
authors.forename: Zhi-gang
authors.initials: ZG
authors.lastname: Wang
mesh_terms: D002478:Cells, Cultured; D003094:Collagen / Q000235:genetics*; D000090602:Collagen Type I, alpha 1 Chain; D004797:Enzyme-Linked Immunosorbent Assay; D000431:Ethanol / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D018793:Interleukin-13 / Q000494:pharmacology*; D053661:Interleukin-13 Receptor alpha1 Subunit / Q000235:genetics; D053663:Interleukin-13 Receptor alpha2 Subunit / Q000235:genetics; D008168:Lung / Q000166:cytology; D011658:Pulmonary Fibrosis / Q000209:etiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: C000716127:COL1A1 protein, human; D000090602:Collagen Type I, alpha 1 Chain; D018793:Interleukin-13; D053661:Interleukin-13 Receptor alpha1 Subunit; D053663:Interleukin-13 Receptor alpha2 Subunit; D000431:Ethanol; D003094:Collagen