title: Intracytoplasmic enzymes in gingival crevicular fluid of patients with aggressive periodontitis.
abstract: OBJECTIVE
  Biochemical parameters of crevicular fluid could provide evidence of periodontal tissue disease. The aim of this study was to analyze enzymes in crevicular fluid in aggressive localized and generalized periodontitis.
  
  
  METHODS
  One hundred and twenty-four subjects were classified as having localized (n = 36) or generalized aggressive periodontitis (n = 38) and subclassified into moderate and severe groups. Controls were 50 periodontitis-free subjects. Activities of the enzymes lactate dehydrogenase, neutrophil elastase, alkaline phosphatase and aspartate aminotransferase were determined. Data were analyzed using one-way ANOVA and Tukey's test.
  
  
  RESULTS
  Among the subjects with localized aggressive periodontitis, values of lactate dehydrogenase and alkaline phosphatase increased notably in moderate and severe periodontitis compared with control subjects. Values for aspartate aminotransferase increased with the severity of the disease, and neutrophil elastase was increased in the moderate and severe states. In generalized aggressive periodontitis, lactate dehydrogenase showed higher values than in control subjects in both periodontal subgroups. Alkaline phosphatase and neutrophil elastase showed higher significant differences between moderate and severe periodontitis compared with the control group. Aspartate aminotransferase showed differences between the severe and moderate periodontitis groups compared with the control group. Of all the enzymes analyzed, only lactate dehydrogenase showed higher values in localized than in generalized aggressive periodontitis.
  
  
  CONCLUSIONS
  Lactate dehydrogenase may distinguish localized and generalized aggressive periodontitis. Alkaline phosphatase increases from moderate to severe states in both types of periodontitis. Aspartate aminotransferase and neutrophil elastase only increase with strong evidence of periodontal destruction.
authors.affiliation: Department of Periodontology, Faculty of Dentistry, National University of Tucumán, San Miguel de Tucumán, Argentina.
authors.forename: C E
authors.initials: CE
authors.lastname: Castro
authors.forename: M A
authors.initials: MA
authors.lastname: Koss
authors.forename: M E
authors.initials: ME
authors.lastname: López
mesh_terms: D000328:Adult; D010520:Aggressive Periodontitis / Q000201:enzymology* / Q000473:pathology; D000469:Alkaline Phosphatase / Q000378:metabolism*; D000704:Analysis of Variance; D001219:Aspartate Aminotransferases / Q000378:metabolism*; D015415:Biomarkers; D016022:Case-Control Studies; D003593:Cytoplasm / Q000201:enzymology; D005260:Female; D005883:Gingival Crevicular Fluid / Q000201:enzymology*; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D019272:Leukocyte Elastase / Q000378:metabolism*; D008297:Male; D012720:Severity of Illness Index; D018709:Statistics, Nonparametric
chemical_list: D015415:Biomarkers; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D000469:Alkaline Phosphatase; D019272:Leukocyte Elastase