title: The impact of mitotic versus interphase chromatin architecture on the molecular flow of EGFP by pair correlation analysis.
abstract: Here we address the impact nuclear architecture has on molecular flow within the mitotic nucleus of live cells as compared to interphase by the pair correlation function method. The mitotic chromatin is found to allow delayed but continuous molecular flow of EGFP in and out of a high chromatin density region, which, by pair correlation function analysis, is shown as a characteristic arc shape that appears upon entry and exit. This is in contrast to interphase chromatin, which regulates flow between different density chromatin regions by means of a mechanism which turns on and off intermittently, generating discrete bursts of EGFP. We show that the interphase bursts are maintained by metabolic energy, whereas the mitotic mechanism of regulation responsible for the arc is not sensitive to ATP depletion. These two distinct routes of molecular flow were concomitantly measured in the Caenorhabditis elegans germ line, which indicates a conservation of mechanism on a scale more widespread than cell type or organism.
authors.affiliation: Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, California, USA.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Hinde
authors.forename: Francesco
authors.initials: F
authors.lastname: Cardarelli
authors.forename: Michelle A
authors.initials: MA
authors.lastname: Digman
authors.forename: Aaron
authors.initials: A
authors.lastname: Kershner
authors.forename: Judith
authors.initials: J
authors.lastname: Kimble
authors.forename: Enrico
authors.initials: E
authors.lastname: Gratton
mesh_terms: D000818:Animals; D001703:Biophysics / Q000379:methods*; D016466:CHO Cells; D017173:Caenorhabditis elegans / Q000166:cytology / Q000378:metabolism; D002843:Chromatin / Q000737:chemistry* / Q000378:metabolism; D006224:Cricetinae; D003412:Cricetulus; D004058:Diffusion; D005854:Germ Cells; D049452:Green Fluorescent Proteins / Q000378:metabolism*; D007399:Interphase; D008938:Mitosis; D021381:Protein Transport
chemical_list: D002843:Chromatin; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins