title: Mucoid Pseudomonas aeruginosa isolates maintain the biofilm formation capacity and the gene expression profiles during the chronic lung infection of CF patients.
abstract: Phenotypic and genotypic diversifications of Pseudomonas aeruginosa in the airways of patients with cystic fibrosis (CF) promote long-term survival of bacteria during chronic lung infection. Twelve clonally related, sequential mucoid and non-mucoid paired P. aeruginosa isolates obtained from three Danish CF patients were investigated. The in vitro biofilm formation capacity was studied under static and flow through conditions and the global gene expression profiles were investigated by Affymetrix GeneChip. Regulatory genes of alginate production and quorum sensing (QS) system were sequenced and measurements of the alginate production and the detection of the QS signal molecules were performed. Comparisons of mucoid and non-mucoid isolates from early and late stages of the infection showed that the mucoid phenotype maintained over a decade the capacity to form in vitro biofilm and showed an unaltered transcriptional profile, whereas substantial alterations in the transcriptional profiles and loss of the capacity to form in vitro biofilms were observed in corresponding isolates of the non-mucoid phenotype. The conserved gene expression pattern in the mucoid isolates vs the diversity of changes in non-mucoid isolates observed in this particular P. aeruginosa clone reflects different adaptation strategies used by these two phenotypes in the different niches of the CF lung environment.
authors.affiliation: Department of International Health, Immunology and Microbiology, Panum Institute, University of Copenhagen, Denmark.
authors.forename: Baoleri
authors.initials: B
authors.lastname: Lee
authors.forename: Charlotte K
authors.initials: CK
authors.lastname: Schjerling
authors.forename: Nikolai
authors.initials: N
authors.lastname: Kirkby
authors.forename: Nadine
authors.initials: N
authors.lastname: Hoffmann
authors.forename: Rehannah
authors.initials: R
authors.lastname: Borup
authors.forename: Søren
authors.initials: S
authors.lastname: Molin
authors.forename: Niels
authors.initials: N
authors.lastname: Høiby
authors.forename: Oana
authors.initials: O
authors.lastname: Ciofu
mesh_terms: D000464:Alginates; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology; D018441:Biofilms; D002908:Chronic Disease; D003550:Cystic Fibrosis / Q000150:complications / Q000382:microbiology*; D003718:Denmark; D020869:Gene Expression Profiling; D005798:Genes, Bacterial; D005838:Genotype; D020723:Glucuronic Acid / Q000096:biosynthesis / Q000235:genetics; D006603:Hexuronic Acids; D006801:Humans; D008171:Lung Diseases / Q000209:etiology / Q000382:microbiology*; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D011552:Pseudomonas Infections / Q000209:etiology / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D053038:Quorum Sensing / Q000235:genetics / Q000502:physiology; D012808:Sigma Factor / Q000235:genetics / Q000502:physiology; D015534:Trans-Activators / Q000235:genetics / Q000502:physiology
chemical_list: C079619:AlgU protein, Pseudomonas aeruginosa; D000464:Alginates; D001426:Bacterial Proteins; D006603:Hexuronic Acids; C080365:LasR protein, Pseudomonas aeruginosa; C107895:MucA protein, Pseudomonas; C086515:RhlR protein, Pseudomonas aeruginosa; D012808:Sigma Factor; D015534:Trans-Activators; D020723:Glucuronic Acid