title: [Occurrence of aminoglycoside transpherase genes in methicillin-resistant strains of Staphylococcus aureus].
abstract: Fifty MRSA strains originated from clinical specimens were examined by the PCR method, for the presence of three genes: aacA-aphD, aadD oraz aph(3")-IIIa. The obtained results were correlated with the susceptibility of the strains to gentamicin, tobramicin, kanamicin, neomicin, amikacin and netilmicin. The susceptibility results were interpreted according with CLSI and EUCAST guidelines. aacA-aphD gene was found in 34 strains, aadD in 27 strains and aph(3")-IIIa was present in 22 strains. In 19 strains (38%) was present one of the investigated genes, in 29 (58%) strains two genes and in two strains (4%) all three genes were found. The most frequent variant was combination of aacA-aphD and aadD genes.
authors.affiliation: Katedra i Zakład Mikrobiologii Lekarskiej Warszawskiego Uniwersytetu Medycznego.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Młynarczyk
authors.forename: Ksenia
authors.initials: K
authors.lastname: Szymanek-Majchrzak
authors.forename: Grazyna
authors.initials: G
authors.lastname: Młynarczyk
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D019868:Kanamycin Kinase / Q000235:genetics*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000145:classification / Q000201:enzymology* / Q000235:genetics*; D009713:Nucleotidyltransferases / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; C562022:AacA-AphD protein, methicillin-resistant Staphylococcus aureus; D019868:Kanamycin Kinase; D009713:Nucleotidyltransferases; C562020:aminoglycoside adenyltransferase AadD, methicillin-resistant Staphylococcus aureus