title: Temporal profiling of the coding and noncoding murine cytomegalovirus transcriptomes.
abstract: The global transcriptional program of murine cytomegalovirus (MCMV), involving coding, noncoding, and antisense transcription, remains unknown. Here we report an oligonucleotide custom microarray platform capable of measuring both coding and noncoding transcription on a genome-wide scale. By profiling MCMV wild-type and immediate-early mutant strains in fibroblasts, we found rapid activation of the transcriptome by 6.5 h postinfection, with absolute dependency on ie3, but not ie1 or ie2, for genomic programming of viral gene expression. Evidence is also presented to show, for the first time, genome-wide noncoding and bidirectional transcription at late stages of MCMV infection.
authors.affiliation: Division of Pathway Medicine, University of Edinburgh Medical School, The Chancellor's Building, 49 Little France Crescent, Edinburgh EH16 4SB, United Kingdom.
authors.forename: Paul
authors.initials: P
authors.lastname: Lacaze
authors.forename: Thorsten
authors.initials: T
authors.lastname: Forster
authors.forename: Alan
authors.initials: A
authors.lastname: Ross
authors.forename: Lorraine E
authors.initials: LE
authors.lastname: Kerr
authors.forename: Eliane
authors.initials: E
authors.lastname: Salvo-Chirnside
authors.forename: Vanda Juranic
authors.initials: VJ
authors.lastname: Lisnic
authors.forename: Guillermo H
authors.initials: GH
authors.lastname: López-Campos
authors.forename: José J
authors.initials: JJ
authors.lastname: García-Ramírez
authors.forename: Martin
authors.initials: M
authors.lastname: Messerle
authors.forename: Joanne
authors.initials: J
authors.lastname: Trgovcich
authors.forename: Ana
authors.initials: A
authors.lastname: Angulo
authors.forename: Peter
authors.initials: P
authors.lastname: Ghazal
mesh_terms: D000818:Animals; D005347:Fibroblasts / Q000821:virology*; D020869:Gene Expression Profiling; D015967:Gene Expression Regulation, Viral; D016679:Genome, Viral; D006566:Herpesviridae Infections / Q000821:virology; D017874:Immediate-Early Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D018146:Muromegalovirus / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity; D009154:Mutation; D041681:NIH 3T3 Cells; D020411:Oligonucleotide Array Sequence Analysis; D013997:Time Factors; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D017874:Immediate-Early Proteins; D014764:Viral Proteins