title: Monoclonal antibody that recognizes a domain on heterogeneous nuclear ribonucleoprotein K and PTB-associated splicing factor.
abstract: hnRNP K protein is a member of the heterogeneous nuclear protein (hnRNP) complex that, besides its function as a translational regulator of human mRNA, is also considered to be a transcription factor involved in tumorigenesis. PSF is a protein part of the human spliceosome and essential in RNA splicing. Here we report the generation of one monoclonal antibody GG6H9.1C3 that recognized both hnRNP K and PSF proteins using Western blot analysis, flow cytometry, and immunocytochemistry.
authors.affiliation: Departamento de Genética, Universidad de Córdoba, Campus de Rabanales, Córdoba, Spain. gemagj76@gmail.com
authors.forename: Gema
authors.initials: G
authors.lastname: Garcia-Jurado
authors.forename: Diego
authors.initials: D
authors.lastname: Llanes
authors.forename: Angela
authors.initials: A
authors.lastname: Moreno
authors.forename: Bernat
authors.initials: B
authors.lastname: Soria
authors.forename: Juan R
authors.initials: JR
authors.lastname: Tejedo
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D015153:Blotting, Western; D002467:Cell Nucleus / Q000378:metabolism; D005434:Flow Cytometry; D034641:Heterogeneous-Nuclear Ribonucleoprotein K / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D000072670:PTB-Associated Splicing Factor; D016601:RNA-Binding Proteins / Q000276:immunology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000911:Antibodies, Monoclonal; D034641:Heterogeneous-Nuclear Ribonucleoprotein K; D000072670:PTB-Associated Splicing Factor; D016601:RNA-Binding Proteins