title: Optimization of cellulase production by a brown rot fungus Fomitopsis sp. RCK2010 under solid state fermentation.
abstract: Culture conditions for enhanced cellulase production from a newly isolated brown rot fungus, Fomitopsis sp. RCK2010 were optimized under solid state fermentation. An initial pH of 5.5 and moisture ratio of 1:3.5 (solid:liquid) were found to be optimal for maximum enzyme production. Of the different carbon sources tested wheat bran gave the maximum production of CMCase (71.526 IU/g), FPase (3.268 IU/g), and β-glucosidase (50.696 IU/g). Among the nitrogen sources, urea caused maximum production of CMCase (81.832 IU/g), where as casein and soyabean meal gave the highest FPase (4.682 IU/g) and β-glucosidase (69.083 IU/g) production, respectively. Among amino acids tested glutamic acid gave the highest production for CMCase (84.127I U/g); however 4-hydroxy-l-proline stimulated maximum FPase production (6.762 IU/g). Saccharification of pretreated rice straw and wheat straw by crude enzyme extract from Fomitopsis sp. RCK2010 resulted in release of 157.160 and 214.044 mg/g of reducing sugar, respectively.
authors.affiliation: Lignocellulose Biotechnology Laboratory, Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, New Delhi 110 021, India.
authors.forename: Deepa
authors.initials: D
authors.lastname: Deswal
authors.forename: Yogender Pal
authors.initials: YP
authors.lastname: Khasa
authors.forename: Ramesh Chander
authors.initials: RC
authors.lastname: Kuhad
mesh_terms: D001483:Base Sequence; D002480:Cellulase / Q000096:biosynthesis*; D055453:Coriolaceae / Q000201:enzymology* / Q000235:genetics; D017931:DNA Primers; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D010802:Phylogeny
chemical_list: D017931:DNA Primers; D002480:Cellulase