title: Raman spectroscopic study of aggregation process of antibiotic amphotericin B induced by H+, Na+, and K+ ions.
abstract: The normal and the preresonance Raman effects (NR and PRR) of spectroscopy have been used to monitor and explain the aggregation processes of amphotericin B (AmB) in aqueous solution at different pH values and containing the K(+) and Na(+) ions. The resonance-enhanced and normal vibrational Raman spectra were recorded with a semiconductor laser (ex 785 nm) and an argon laser (ex 514.5 nm) for investigation of interactions between AmB chromophores. The essential difference between the samples stimulated by resonance-enhanced and by near-infrared was in the C=C stretching mode region of polyene chain. The processes connected with the aggregation of AmB led to changes in the chromophore, which were only visible as a remarkable broadening of the band centered at 1558 cm(-1). The understanding of possible physical mechanisms responsible for the molecular aggregation of the drug is important from the pharmaceutical applicability standpoint.
authors.affiliation: Department of Biophysics, University of Life Sciences in Lublin, 20-950 Lublin, Poland. mariusz.gagos@up.lublin.pl
authors.forename: Mariusz
authors.initials: M
authors.lastname: Gagoś
authors.forename: Marta
authors.initials: M
authors.lastname: Arczewska
authors.forename: Wiesław I
authors.initials: WI
authors.lastname: Gruszecki
mesh_terms: D000666:Amphotericin B / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007477:Ions / Q000737:chemistry; D008968:Molecular Conformation; D011188:Potassium / Q000737:chemistry*; D012964:Sodium / Q000737:chemistry*; D012996:Solutions; D013059:Spectrum Analysis, Raman; D014867:Water / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D007477:Ions; D012996:Solutions; D014867:Water; D000666:Amphotericin B; D012964:Sodium; D011188:Potassium