title: Helicobacter pylori infection promotes methylation of WWOX gene in human gastric cancer.
abstract: WW domain-containing oxidoreductase (WWOX), a tumor suppressor gene, was reported to be downregulated in gastric cancer and other tumors. However, the mechanism by which WWOX is inactivated remains unclear. In our study, methylation status of WWOX was determined by MSP and sequencing. Our results showed that WWOX hypermethylation was frequently detected in gastric cancer, and also significantly correlated with Helicobacter pylori (H. pylori) infection. Promoter methylation of WWOX was induced in BCG823 and AGS cells co-cultured with H. pylori. Finally, we found that expression of DNMT1 and DNMT3A were enhanced when cells were co-cultured with H. pylori. Our study indicated that H. pylori infection promoted methylation of WWOX gene in gastric cancer.
authors.affiliation: Department of Oncology, The First Affiliated Hospital, Zhengzhou University, People's Republic of China.
authors.forename: Jiaqin
authors.initials: J
authors.lastname: Yan
authors.forename: Mingzhi
authors.initials: M
authors.lastname: Zhang
authors.forename: Junhui
authors.initials: J
authors.lastname: Zhang
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Chen
authors.forename: Xudong
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000096:biosynthesis; D019175:DNA Methylation; D000090205:DNA Methyltransferase 3A; D016481:Helicobacter Infections / Q000150:complications / Q000235:genetics*; D016480:Helicobacter pylori; D006801:Humans; D010088:Oxidoreductases / Q000235:genetics*; D013274:Stomach Neoplasms / Q000235:genetics* / Q000382:microbiology*; D014407:Tumor Cells, Cultured; D025521:Tumor Suppressor Proteins / Q000235:genetics*; D000074584:WW Domain-Containing Oxidoreductase
chemical_list: C000717967:DNMT3A protein, human; D025521:Tumor Suppressor Proteins; D010088:Oxidoreductases; D000074584:WW Domain-Containing Oxidoreductase; C486071:WWOX protein, human; D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases; D000090205:DNA Methyltransferase 3A; C000619380:DNMT1 protein, human