title: iTRAQ-based proteomic study of the effects of microcystin-LR on medaka fish liver.
abstract: Microcystins are cyanotoxins that occur in ground water and thus pose a potential health risk. Microcystin-LR (microcystin-leucine-arginine) is a potent hepatotoxin, and is suspected of being a tumour promoter. Poisoning with this toxin causes several dysfunctions in hepatocytes by inhibiting protein phosphatases 1 and 2A, and notably produces oxidative stress, disrupts the cytoskeleton, and deregulates mitogen-activated protein kinase pathway. Medaka fish (Oryzias latipes) was chosen as a model for studying the effects of this cyanotoxin on liver proteins using a gel-free approach, iTRAQ. Fish were gavaged with microcystin-LR. Two hours later, 325 proteins could be identified by Scaffold Q+ and 32 proteins revealed statistically significant variations above a ∣0.2∣ threshold of log(2) ratio by comparison with control. These proteins are mostly involved in the translation and maturation of proteins, lipid metabolism and detoxification. Notably, apolipoproteins are deregulated which indicates a possible alteration of chylomicron-mediated transport.
authors.affiliation: CNRS, FRE 3206 Molécules de communication et adaptation des microorganismes, Département Régulations, développement et diversité moléculaire, Muséum national d'Histoire naturelle, Paris, France.
authors.forename: Mélodie
authors.initials: M
authors.lastname: Malécot
authors.forename: Arul
authors.initials: A
authors.lastname: Marie
authors.forename: Simone
authors.initials: S
authors.lastname: Puiseux-Dao
authors.forename: Marc
authors.initials: M
authors.lastname: Edery
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001427:Bacterial Toxins / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D005260:Female; D029941:Fish Proteins / Q000032:analysis / Q000145:classification / Q000378:metabolism; D007553:Isotope Labeling / Q000379:methods; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008387:Marine Toxins / Q000494:pharmacology; D052998:Microcystins / Q000494:pharmacology*; D009990:Oryzias / Q000378:metabolism*; D020543:Proteome / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D018709:Statistics, Nonparametric; D053719:Tandem Mass Spectrometry
chemical_list: D001427:Bacterial Toxins; D029941:Fish Proteins; D008387:Marine Toxins; D052998:Microcystins; D020543:Proteome; C057862:cyanoginosin LR