title: Evaluation of a multiarray system for pharmaceutical analysis by microemulsion electrokinetic chromatography.
abstract: A multiplexed capillary electrophoresis (CE) system equipped with 96 channels was evaluated for high-throughput screening in drug discovery by microemulsion electrokinetic chromatography (MEEKC). Method transfer from a single channel to a multichannel CE system is described. Loss of efficiency and reduced migration times could be elucidated to the poor efficacy in Joule heat dissipation by forced air cooling in the multiarray system compared to liquid cooling in the single channel instrument. On the other hand, only 48 channels could actually be used because of the maximum total current of 3 mA. Precision data remained below 8% and 9% for migration times and peak areas, respectively. Some UV-detector cross-talk interference between neighboring capillary channels was noted. Impurities at 0.5% compared to the main peak (100%) could be detected with the multiplexed system which is 10 times lower compared to the single capillary system. Higher efficiency and improved figures of merit (absolute sensitivity and no cross-talk interferences) were obtained by using an array of only 24 capillaries.
authors.affiliation: Pfizer Analytical Research Centre, Ghent University, Krijgslaan 281 S4-bis, B-9000 Ghent, Belgium.
authors.forename: Frederic
authors.initials: F
authors.lastname: Lynen
authors.forename: Luis
authors.initials: L
authors.lastname: Saavedra
authors.forename: Luis
authors.initials: L
authors.lastname: Saveedra
authors.forename: Beverly
authors.initials: B
authors.lastname: Nickerson
authors.forename: Pat
authors.initials: P
authors.lastname: Sandra
mesh_terms: D020374:Chromatography, Micellar Electrokinetic Capillary / Q000592:standards*; D004364:Pharmaceutical Preparations / Q000032:analysis*; D015203:Reproducibility of Results
chemical_list: D004364:Pharmaceutical Preparations