title: [Expression of high mobility group box chromosomal protein 1 in mice with lupus nephritis].
abstract: To determine the role of the novel proinflammatory cytokine high mobility group box chromosomal protein 1 (HMGB-1) in the pathogenesis of lupus nephritis.
  
  
  
  Serum levels of anti-dsDNA antibodies were determined by enzyme linked immunosorbent assay (ELISA). Renal morphologic features were examined by light microscopy, electron microscopy, and immunohistologic analyses. The mRNA expression of HMGB-1 and monocyte chemoattractant protein-1 (MCP-1) was detected by RT-PCR.
  
  
  
  MRL/lpr mice demonstrated characteristic alterations of serum immune parameters, with progressively increased anti-dsDNA antibodies with age, compared with age-matched C57BL/6J mice. MRL/lpr mice showed progressive development of renal damage, starting at 12 weeks of age and reached the peak at 20 weeks. The observed lesions included the presence of enlarged hypercellular glomeruli, with increased numbers of both resident cells and infiltrating leukocytes. Higher expression of HMGB-1 mRNA was found in MRL/lpr mice than what in C57BL/6J mice. Expression of HMGB-1 was positively correlated with that of MCP-1 mRNA.
  
  
  
  The results demonstrate that the higher expression of HMGB-1 may contribute to the pathogenesis of lupus nephritis.
authors.affiliation: Zhejiang Provincial Hospital of Traditional Chinese Medicine, Hangzhou 310006, China. jianguangzhou@gmail.com
authors.forename: Jian-Guang
authors.initials: JG
authors.lastname: Zhou
authors.forename: Jing-Yin
authors.initials: JY
authors.lastname: Dong
authors.forename: Li-Huang
authors.initials: LH
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D018932:Chemokine CCL2 / Q000378:metabolism; D004195:Disease Models, Animal; D024243:HMGB1 Protein / Q000235:genetics / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism / Q000473:pathology; D008181:Lupus Nephritis / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D019463:Mice, Inbred MRL lpr; D012333:RNA, Messenger / Q000235:genetics
chemical_list: C491768:Ccl2 protein, mouse; D018932:Chemokine CCL2; D024243:HMGB1 Protein; D012333:RNA, Messenger