title: [Construstion of IL3-PE38KDEL fusion gene eukaryolic expression vector and its expression in pichia].
abstract: OBJECTIVE
  to construct a eukaryolic expression plasmid of Ppic9k-IL3-Linker-PE38KDE.
  
  
  METHODS
  the IL3 and PE38KDEL gene were amplified by polymerase chain reaction (PCR) and cloned into the eukaryolic expression plasmid Ppic9k-Linker constructed after being sequenced.The recombinant vector confirmed by restriction endonucleases digestion, coenobium PCR and DNA sequence analysis showed that the prokaryotic expression vector Ppic9k-IL3-Linker-PE38KDEL was constructed successfully.
  
  
  CONCLUSIONS
  the fusion gene IL3-PE38KDEL is successfully constructed, which laid a solid foundation for the further research.
authors.affiliation: Department of Hematology, the Second Affiliated Hospital, Chongqing Medical University, Chongping, China.
authors.forename: Feng-xia
authors.initials: FX
authors.lastname: Bai
authors.forename: Shi-feng
authors.initials: SF
authors.lastname: Lou
mesh_terms: D014408:Biomarkers, Tumor / Q000737:chemistry / Q000235:genetics; D003001:Cloning, Molecular / Q000379:methods; D005822:Genetic Vectors / Q000138:chemical synthesis* / Q000737:chemistry / Q000235:genetics; D006801:Humans; D000922:Immunotoxins / Q000737:chemistry* / Q000276:immunology; D007377:Interleukin-3 / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D007938:Leukemia / Q000473:pathology / Q000628:therapy; D010843:Pichia / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods
chemical_list: D014408:Biomarkers, Tumor; D000922:Immunotoxins; D007377:Interleukin-3; D011993:Recombinant Fusion Proteins