title: [Preparation and antithrombogenicity of oxidated low molecular weight heparin-antithrombin complex coated-polyvinyl chloride tubing].
abstract: Based on non-enzymatic protein glycated reaction, the sodium periodate-oxidated low molecular weight heparin-antithrombin covalent complex (SPLMWATH) was produced. By using polyethyleneimine-glutaraldehyde bonding technique, polyvinyl chloride (PVC) tubings were coated with SPLMWATH, heparin and low molecular weight heparin (LMWH). Spectrophotometry and dynamic clotting time experiment were used to determine the synthetic ratio of SPLMWATH, graft density, coating leaching ratio and to evaluate the antithrombogenicity of different coating on the PVC tubings. The results showed that the synthetic ratio of SPLMWATH was approximately 55%, and compared with heparin coating and LMWH coating, the graft density of SPLMWATH coating on the PVC tubing was smaller, but its coating stability and antithrombogenicity were significantly better than that of heparin coating and LMWH coating on the PVC tubings.
authors.affiliation: Department of Cardiovascular Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710033, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Luo
authors.forename: Weiyong
authors.initials: W
authors.lastname: Liu
authors.forename: Chun
authors.initials: C
authors.lastname: Yang
authors.forename: Hua
authors.initials: H
authors.lastname: Zhou
authors.forename: Ruijun
authors.initials: R
authors.lastname: Cao
authors.forename: Jian
authors.initials: J
authors.lastname: Yang
mesh_terms: D000925:Anticoagulants / Q000138:chemical synthesis* / Q000494:pharmacology; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis* / Q000494:pharmacology; D005112:Extracorporeal Circulation / Q000295:instrumentation*; D006495:Heparin, Low-Molecular-Weight / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D011143:Polyvinyl Chloride / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D000925:Anticoagulants; D020099:Coated Materials, Biocompatible; D006495:Heparin, Low-Molecular-Weight; D011143:Polyvinyl Chloride