title: Migration of cytotoxic lymphocytes in cell cycle permits local MHC I-dependent control of division at sites of viral infection.
abstract: After virus infection, cytotoxic T lymphocytes (CTLs) divide rapidly to eradicate the pathogen and prevent the establishment of persistence. The magnitude of an antiviral CTL response is thought to be controlled by the initiation of a cell cycle program within lymphoid tissues. However, it is presently not known whether this division program proceeds during migration or is influenced locally at sites of viral infection. We demonstrate that antiviral CTLs remain in cell cycle while transiting to infected tissues. Up to one third of virus-specific CTLs within blood were found to be in cell cycle after infection with lymphocytic choriomeningitis virus or vesicular stomatitis virus. Using two-photon microscopy, we found that effector CTL divided rapidly upon arrest in the virus-infected central nervous system as well as in meningeal blood vessels. We also observed that MHC I-dependent interactions, but not costimulation, influenced the division program by advancing effector CTL through stages of the cell cycle. These results demonstrate that CTLs are poised to divide in transit and that their numbers can be influenced locally at the site of infection through interactions with cells displaying cognate antigen.
authors.affiliation: National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: Silvia S
authors.initials: SS
authors.lastname: Kang
authors.forename: Jasmin
authors.initials: J
authors.lastname: Herz
authors.forename: Jiyun V
authors.initials: JV
authors.lastname: Kim
authors.forename: Debasis
authors.initials: D
authors.lastname: Nayak
authors.forename: Phillip
authors.initials: P
authors.lastname: Stewart-Hutchinson
authors.forename: Michael L
authors.initials: ML
authors.lastname: Dustin
authors.forename: Dorian B
authors.initials: DB
authors.lastname: McGavern
mesh_terms: D000069594:Abatacept; D000818:Animals; D001483:Base Sequence; D002453:Cell Cycle; D002455:Cell Division; D002465:Cell Movement; D003713:Dendritic Cells / Q000502:physiology; D005805:Genes, MHC Class I / Q000502:physiology*; D018796:Immunoconjugates / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D013602:T-Lymphocytes, Cytotoxic / Q000502:physiology*; D014777:Virus Diseases / Q000276:immunology*
chemical_list: D018796:Immunoconjugates; D000069594:Abatacept