title: Orchardgrass (Dactylis glomerata L.) EST and SSR marker development, annotation, and transferability.
abstract: Orchardgrass, or cocksfoot [Dactylis glomerata (L.)], has been naturalized on nearly every continent and is a commonly used species for forage and hay production. All major cultivated varieties of orchardgrass are autotetraploid, and few tools or information are available for functional and comparative genetic analyses and improvement of the species. To improve the genetic resources for orchardgrass, we have developed an EST library and SSR markers from salt, drought, and cold stressed tissues. The ESTs were bi-directionally sequenced from clones and combined into 17,373 unigenes. Unigenes were annotated based on putative orthology to genes from rice, Triticeae grasses, other Poaceae, Arabidopsis, and the non-redundant database of the NCBI. Of 1,162 SSR markers developed, approximately 80% showed amplification products across a set of orchardgrass germplasm, and 40% across related Festuca and Lolium species. When orchardgrass subspecies were genotyped using 33 SSR markers their within-accession similarity values ranged from 0.44 to 0.71, with Mediterranean accessions having a higher similarity. The total number of genotyped bands was greater for tetraploid accessions compared to diploid accessions. Clustering analysis indicated grouping of Mediterranean subspecies and central Asian subspecies, while the D. glomerata ssp. aschersoniana was closest related to three cultivated varieties.
authors.affiliation: USDA-ARS Forage and Range Research Lab, 695 N 1100 E, Logan, UT 84322-6300, USA. shaun.bushman@ars.usda.gov
authors.forename: B Shaun
authors.initials: BS
authors.lastname: Bushman
authors.forename: Steve R
authors.initials: SR
authors.lastname: Larson
authors.forename: Metin
authors.initials: M
authors.lastname: Tuna
authors.forename: Mark S
authors.initials: MS
authors.lastname: West
authors.forename: Alvaro G
authors.initials: AG
authors.lastname: Hernandez
authors.forename: Deepika
authors.initials: D
authors.lastname: Vullaganti
authors.forename: George
authors.initials: G
authors.lastname: Gong
authors.forename: Joseph G
authors.initials: JG
authors.lastname: Robins
authors.forename: Kevin B
authors.initials: KB
authors.lastname: Jensen
authors.forename: Jyothi
authors.initials: J
authors.lastname: Thimmapuram
mesh_terms: D000595:Amino Acid Sequence; D002874:Chromosome Mapping; D016000:Cluster Analysis; D031745:Dactylis / Q000235:genetics*; D020224:Expressed Sequence Tags; D031751:Festuca / Q000235:genetics; D015723:Gene Library; D018014:Gene Transfer Techniques; D017343:Genes, Plant; D005819:Genetic Markers; D005838:Genotype; D008129:Lolium / Q000235:genetics; D058977:Molecular Sequence Annotation; D011110:Polymorphism, Genetic; D057891:Tetraploidy
chemical_list: D005819:Genetic Markers