title: [Virus-induced gene silencing as a tool for analysis of gene functions in plants].
abstract: Virus-induced gene silencing (VIGS) is a technology that exploits an RNA-mediated antivirus defense mechanism in plants and has been shown to have great potential in plant reverse genetics. When the virus vector carries sequences of plant genes, virus infection triggers VIGS that results in the degradation of endogenous mRNAs homologous to the plant genes. The system is well established in Nicotiana benthamiana and several reliable VIGS vectors have been developed for other plant species including important agricultural crops. Here, we describe the use of VIGS technology to determine gene function and plant virus vectors for induction of VIGS in plants.
authors.affiliation: Faculty of Agriculture, Iwate University Morioka 020-8550, Japan.
authors.forename: Noriko
authors.initials: N
authors.lastname: Yamagishi
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Yoshikawa
mesh_terms: D017343:Genes, Plant / Q000502:physiology*; D005821:Genetic Techniques; D005822:Genetic Vectors; D059014:High-Throughput Nucleotide Sequencing; D010942:Plant Viruses / Q000235:genetics*; D010944:Plants / Q000235:genetics*; D034622:RNA Interference