title: Synthesis, characterization, and in vivo biodistribution of 125I-labeled Dex-g-PMAGGCONHTyr.
abstract: Dextran graft poly (N-methacryloylglycylglycine) copolymer-tyrosine conjugates (dextran-g-PMAGGCONHTyr) were synthesized and characterized. Dynamic light scattering (DLS) results indicated that the graft copolymers are soluble in pH 7.4 PBS and 0.9% saline solutions. The graft copolymers were labeled with (125)I, and the labeling stability in 0.9% saline solution was investigated. Pharmacokinetics studies showed a rapid clearance of (125)I-labeled graft copolymers from the blood pool. Biodistribution images confirmed the preferable liver and spleen accumulation within 1 h after injection and rapid clearance from all the organs over time. The graft copolymer with molecular weight of 9.8 kDa was eliminated from the kidney significantly faster than those with higher molecular weight. The effect of the numbers of -COOH groups on the graft copolymers on the biodistribution was also investigated. It was found that the graft copolymers with the average number of -COOH groups per glucopyranose unit (DS(-COOH)) of 0.57 and 0.18 are mainly distributed in liver and spleen at 1 h after injection, whereas the graft copolymer with DS(-COOH) of 0.07 is mainly accumulated in kidney.
authors.affiliation: Laboratory of Polymer Physics and Chemistry, Beijing National Laboratory of Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, China.
authors.forename: Deqian
authors.initials: D
authors.lastname: Wang
authors.forename: Jiyun
authors.initials: J
authors.lastname: Shi
authors.forename: Junjun
authors.initials: J
authors.lastname: Tan
authors.forename: Xin
authors.initials: X
authors.lastname: Jin
authors.forename: Qinmei
authors.initials: Q
authors.lastname: Li
authors.forename: Honglang
authors.initials: H
authors.lastname: Kang
authors.forename: Ruigang
authors.initials: R
authors.lastname: Liu
authors.forename: Bing
authors.initials: B
authors.lastname: Jia
authors.forename: Yong
authors.initials: Y
authors.lastname: Huang
mesh_terms: D007457:Iodine Radioisotopes / Q000493:pharmacokinetics*; D009682:Magnetic Resonance Spectroscopy; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry / Q000493:pharmacokinetics*; D014018:Tissue Distribution
chemical_list: D007457:Iodine Radioisotopes; D011108:Polymers