title: Development of bioengineered human larynx.
abstract: To date, only two human laryngeal allotransplants have been reported and, although they were successful, both patients required life-long immunosuppression. A bioengineered human larynx could represent a possible alternative to allotransplantation. Human larynxes were decellularized enzymatically to obtain acellular matrices. Histological and molecular analysis demonstrated that all cellular components and nuclear material were removed. SEM showed that decellularized matrices retained the hierarchical structures of the native larynx, and mechanical tests demonstrated that the decellularization did not significantly impaired the biomechanically properties of the obtained matrices. Immunohistochemical staining found residual angiogenic factors after decellularization, and CAM analysis demonstrated that acellular laryngeal scaffolds induce a strong in vivo angiogenic response. Using a decellularization method, we are now able to obtain, in a short and clinically useful time, natural bioengineered laryngeal scaffolds which could be use for partial or total implantation in humans.
authors.affiliation: BIOAIRLab, University Hospital Careggi, Florence, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Baiguera
authors.forename: Alessandro
authors.initials: A
authors.lastname: Gonfiotti
authors.forename: Massimo
authors.initials: M
authors.lastname: Jaus
authors.forename: Camilla E
authors.initials: CE
authors.lastname: Comin
authors.forename: Milena
authors.initials: M
authors.lastname: Paglierani
authors.forename: Costantino
authors.initials: C
authors.lastname: Del Gaudio
authors.forename: Alessandra
authors.initials: A
authors.lastname: Bianco
authors.forename: Domenico
authors.initials: D
authors.lastname: Ribatti
authors.forename: Paolo
authors.initials: P
authors.lastname: Macchiarini
mesh_terms: D000328:Adult; D000818:Animals; D057005:Bioengineering / Q000379:methods*; D001681:Biological Assay / Q000379:methods; D001696:Biomechanical Phenomena; D002102:Cadaver; D002645:Chickens; D005260:Female; D006801:Humans; D007830:Larynx / Q000033:anatomy & histology / Q000737:chemistry; D008297:Male; D008422:Materials Testing; D008875:Middle Aged; D018919:Neovascularization, Physiologic; D013314:Stress, Mechanical; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds