title: Diversity and evolution of four dispersed repetitive DNA sequences in the genus Secale.
abstract: We present the first characterization of 360 sequences in six species of the genus Secale of both cultivated and wild accessions. These include four distinct kinds of dispersed repetitive DNA sequences named pSc20H, pSc119.1, pSaO5(411), and pSaD15(940) belonging to the Revolver family. During the evolution of the genus Secale from wild to cultivated accessions, the pSaO5(411)-like sequences became shorter mainly because of the deletion of a trinucleotide tandem repeating unit, the pSc20H-like sequences displayed apparent homogenization in cultivated rye, and the second intron of Revolver became longer. In addition, the pSc20H-, pSc119.1-, and pSaO5(411)-like sequences cloned from wild rye and cultivated rye could be divided into two large clades. No single case of the four kinds of repetitive elements has been inherited by each Secale accession from a lone ancestor. It is reasonable to consider the vertical transmission of the four repetitive elements during the evolution of the genus Secale. The pSc20H- and pSaO5(411)-like sequences showed evolutionary elimination at specific chromosomal locations from wild species to cultivated species. These cases imply that different repetitive DNA sequences have played different roles in the chromosome development and genomic evolution of rye. The present study adds important information to the investigations dealing with characterization of dispersed repetitive elements in wild and cultivated rye.
authors.affiliation: State Key Laboratory of Plant Breeding and Genetics, Sichuan Agriculture University, Ya'an, Sichuan 625014, China.
authors.forename: Zong-Xiang
authors.initials: ZX
authors.lastname: Tang
authors.forename: Shu-Lan
authors.initials: SL
authors.lastname: Fu
authors.forename: Zheng-Long
authors.initials: ZL
authors.lastname: Ren
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.forename: Yu-Ting
authors.initials: YT
authors.lastname: Zou
authors.forename: Zu-Jun
authors.initials: ZJ
authors.lastname: Yang
authors.forename: Guang-Rong
authors.initials: GR
authors.lastname: Li
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Zhou
authors.forename: Huai-Qiong
authors.initials: HQ
authors.lastname: Zhang
authors.forename: Ben-Ju
authors.initials: BJ
authors.lastname: Yan
authors.forename: Huai-Yu
authors.initials: HY
authors.lastname: Zhang
authors.forename: Fei-Quan
authors.initials: FQ
authors.lastname: Tan
mesh_terms: D032461:Chromosomes, Plant / Q000235:genetics; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000737:chemistry / Q000145:classification / Q000235:genetics; D019143:Evolution, Molecular; D014644:Genetic Variation; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D012434:Secale / Q000145:classification / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D018744:DNA, Plant