title: CycADS: an annotation database system to ease the development and update of BioCyc databases.
abstract: In recent years, genomes from an increasing number of organisms have been sequenced, but their annotation remains a time-consuming process. The BioCyc databases offer a framework for the integrated analysis of metabolic networks. The Pathway tool software suite allows the automated construction of a database starting from an annotated genome, but it requires prior integration of all annotations into a specific summary file or into a GenBank file. To allow the easy creation and update of a BioCyc database starting from the multiple genome annotation resources available over time, we have developed an ad hoc data management system that we called Cyc Annotation Database System (CycADS). CycADS is centred on a specific database model and on a set of Java programs to import, filter and export relevant information. Data from GenBank and other annotation sources (including for example: KAAS, PRIAM, Blast2GO and PhylomeDB) are collected into a database to be subsequently filtered and extracted to generate a complete annotation file. This file is then used to build an enriched BioCyc database using the PathoLogic program of Pathway Tools. The CycADS pipeline for annotation management was used to build the AcypiCyc database for the pea aphid (Acyrthosiphon pisum) whose genome was recently sequenced. The AcypiCyc database webpage includes also, for comparative analyses, two other metabolic reconstruction BioCyc databases generated using CycADS: TricaCyc for Tribolium castaneum and DromeCyc for Drosophila melanogaster. Linked to its flexible design, CycADS offers a powerful software tool for the generation and regular updating of enriched BioCyc databases. The CycADS system is particularly suited for metabolic gene annotation and network reconstruction in newly sequenced genomes. Because of the uniform annotation used for metabolic network reconstruction, CycADS is particularly useful for comparative analysis of the metabolism of different organisms. Database URL: http://www.cycadsys.org.
authors.affiliation: UMR203 BF2I, Biologie Fonctionnelle Insectes et Interactions, INRA, INSA-Lyon, Université de Lyon, 20 av. A. Einstein, F-69621 Villeurbanne, France. augusto@cycadsys.org
authors.forename: Augusto F
authors.initials: AF
authors.lastname: Vellozo
authors.forename: Amélie S
authors.initials: AS
authors.lastname: Véron
authors.forename: Patrice
authors.initials: P
authors.lastname: Baa-Puyoulet
authors.forename: Jaime
authors.initials: J
authors.lastname: Huerta-Cepas
authors.forename: Ludovic
authors.initials: L
authors.lastname: Cottret
authors.forename: Gérard
authors.initials: G
authors.lastname: Febvay
authors.forename: Federica
authors.initials: F
authors.lastname: Calevro
authors.forename: Yvan
authors.initials: Y
authors.lastname: Rahbé
authors.forename: Angela E
authors.initials: AE
authors.lastname: Douglas
authors.forename: Toni
authors.initials: T
authors.lastname: Gabaldón
authors.forename: Marie-France
authors.initials: MF
authors.lastname: Sagot
authors.forename: Hubert
authors.initials: H
authors.lastname: Charles
authors.forename: Stefano
authors.initials: S
authors.lastname: Colella
mesh_terms: D000465:Algorithms; D000818:Animals; D030541:Databases, Genetic; D023281:Genomics / Q000379:methods / Q000706:statistics & numerical data*; D006801:Humans; D020407:Internet; D053858:Metabolic Networks and Pathways; D058977:Molecular Sequence Annotation / Q000379:methods / Q000706:statistics & numerical data*; D012984:Software