title: A novel in vivo cell-wall labeling approach sheds new light on peptidoglycan synthesis in Escherichia coli.
abstract: Peptidoglycan synthesis and turnover in relation to cell growth and division has been studied by using a new labeling method. This method involves the incorporation of fluorescently labeled peptidoglycan precursors into the cell wall by means of the cell-wall recycling pathway. We show that Escherichia coli is able to import exogenous added murein tripeptide labeled with N-7-nitro-2,1,3-benzoxadiazol-4-yl (AeK-NBD) into the cytoplasm where it enters the peptidoglycan biosynthesis route, resulting in fluorescent labels specifically located in the cell wall. When wild-type cells were grown in the presence of the fluorescent peptide, peptidoglycan was uniformly labeled in cells undergoing elongation. Cells in the process of division displayed a lack of labeled peptidoglycan at mid-cell. Analysis of labeling patterns in cell division mutants showed that the occurrence of unlabeled peptidoglycan is dependent on the presence of FtsZ, but independent of FtsQ and FtsI. Accumulation of fluorescence at the division sites of a triple amidase mutant (ΔamiABC) revealed that AeK-NBD is incorporated into septal peptidoglycan. AmiC was shown to be involved in the rapid removal of labeled peptidoglycan side chains at division sites in wild-type cells. Because septal localization of AmiC is dependent on FtsQ and FtsI, this points to the presence of another peptidoglycan hydrolase activity directly dependent on FtsZ.
authors.affiliation: Department of Chemical Biology and Organic Chemistry, University of Utrecht, The Netherlands.
authors.forename: Nick K
authors.initials: NK
authors.lastname: Olrichs
authors.forename: Mirjam E G
authors.initials: ME
authors.lastname: Aarsman
authors.forename: Jolanda
authors.initials: J
authors.lastname: Verheul
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Arnusch
authors.forename: Nathaniel I
authors.initials: NI
authors.lastname: Martin
authors.forename: Mireille
authors.initials: M
authors.lastname: Hervé
authors.forename: Waldemar
authors.initials: W
authors.lastname: Vollmer
authors.forename: Ben
authors.initials: B
authors.lastname: de Kruijff
authors.forename: Eefjan
authors.initials: E
authors.lastname: Breukink
authors.forename: Tanneke
authors.initials: T
authors.lastname: den Blaauwen
mesh_terms: D002473:Cell Wall / Q000737:chemistry* / Q000378:metabolism; D004926:Escherichia coli / Q000166:cytology / Q000378:metabolism*; D010457:Peptidoglycan / Q000096:biosynthesis* / Q000737:chemistry; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D010457:Peptidoglycan