title: Usefulness of a genotypic resistance test using dried blood spot specimens in African HIV-infected children with virological failure according to the 2010-revised WHO criteria.
abstract: We compared paired plasma and dried blood spot (DBS) samples from 54 HIV-1-treated children living in Bangui, Central African Republic, for antiretroviral-resistance-associated mutations. All children displayed virological failure (HIV-1 RNA >3.70 log(10)copies/ml). Testing for resistance genotype was carried out in a reference laboratory in Paris, France. A successful test result was obtained in 54 (100%) plasmas and 25 DBSs (46%). Among the 732 resistance-associated mutations analyzed, 718 were identical, leading to a high concordance rate of 98.1%. Genotypic resistance tests on DBS samples were found to be highly feasible and accurate in a foreign reference laboratory, but with additional costs for shipping and decreased sensitivity.
authors.affiliation: Laboratoire de Virologie, Assistance Publique-Hôpitaux de Paris (AP-HP), Hôpital Européen Georges Pompidou, Université Paris Descartes, Paris 5, 20 rue Leblanc, 75015 Paris, France. charlotte.charpentier@bch.aphp.fr
authors.forename: Charlotte
authors.initials: C
authors.lastname: Charpentier
authors.forename: Jean-Chrysostome
authors.initials: JC
authors.lastname: Gody
authors.forename: Pascaline
authors.initials: P
authors.lastname: Tisserand
authors.forename: Mathieu
authors.initials: M
authors.lastname: Matta
authors.forename: Julien
authors.initials: J
authors.lastname: Fournier
authors.forename: Olivia
authors.initials: O
authors.lastname: Mbitikon
authors.forename: Laurent
authors.initials: L
authors.lastname: Bélec
mesh_terms: D019380:Anti-HIV Agents / Q000627:therapeutic use*; D001800:Blood Specimen Collection / Q000379:methods; D002488:Central African Republic / Q000453:epidemiology; D002648:Child; D024882:Drug Resistance, Viral / Q000235:genetics*; D005838:Genotype; D015658:HIV Infections / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology*; D015497:HIV-1 / Q000187:drug effects* / Q000235:genetics*; D006801:Humans; D012367:RNA, Viral / Q000097:blood / Q000235:genetics; D017211:Treatment Failure
chemical_list: D019380:Anti-HIV Agents; D012367:RNA, Viral