title: Hydrophilic interaction liquid chromatography: ESI-MS/MS of plasmalogen phospholipids from Pectinatus bacterium.
abstract: Liquid chromatography-electrospray tandem mass spectrometry (LC/ESI-MS/MS) was used to analyze phospholipids from three species of the anaerobic beer-spoilage bacterial genus Pectinatus. Analysis of total lipids by HILIC (Hydrophilic Interaction Liquid Chromatography) column succeeded in separating diacyl- and plasmalogen phospholipids. Plasmalogens were then analyzed by means of the ESI-MS/MS and more than 220 molecular species of four classes of plasmalogens (PlsCho (choline plasmalogen), PlsEtn (ethanolamine plasmalogen), PlsGro (glycerol plasmalogen), and PlsSer (serine plasmalogen)) were identified. Major molecular species were c-p19:0/15:0 PlsEtn and PlsSer, which accounted for more than 4% of the total lipids.
authors.affiliation: Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20, Prague, Czech Republic. rezanka@biomed.cas.cz
authors.forename: Tomáš
authors.initials: T
authors.lastname: Rezanka
authors.forename: Lucie
authors.initials: L
authors.lastname: Siristova
authors.forename: Dagmar
authors.initials: D
authors.lastname: Matoulková
authors.forename: Karel
authors.initials: K
authors.lastname: Sigler
mesh_terms: D000447:Aldehydes / Q000737:chemistry; D000818:Animals; D001515:Beer / Q000382:microbiology; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005227:Fatty Acids / Q000737:chemistry; D006801:Humans; D015394:Molecular Structure; D045852:Pectinatus / Q000737:chemistry*; D010743:Phospholipids / Q000032:analysis*; D010955:Plasmalogens / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000447:Aldehydes; D005227:Fatty Acids; D010743:Phospholipids; D010955:Plasmalogens