title: High-throughput analysis of glycoproteins from plasma.
abstract: The glycoproteome is a major subproteome of plasma. To identify low-abundance plasma glycoproteins and specific glycoforms as disease biomarkers, high-throughput and reproducible methods for glycoproteomic analysis are needed. A glycoproteomic method, utilising solid-phase extraction of glycopeptides (SPEG) based on chemical immobilization of glycopeptides has been developed. The step-by-step protocol for this high-throughput SPEG method using multichannel liquid handling system is described. The protocol provides efficient enrichment for glycoproteins for proteomic identification using mass spectrometry.
authors.affiliation: Department of Pathology, Division of Clinical Chemistry, John Hopkins University, Baltimore, MD, USA.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
mesh_terms: D002851:Chromatography, High Pressure Liquid; D006020:Glycopeptides / Q000097:blood / Q000737:chemistry; D006023:Glycoproteins / Q000097:blood* / Q000737:chemistry; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D013058:Mass Spectrometry; D052616:Solid Phase Extraction
chemical_list: D006020:Glycopeptides; D006023:Glycoproteins