title: Evaluation of Chinese medicinal herbs fingerprinting by HPLC-DAD for the detection of toxic aristolochic acids.
abstract: Aristolochic acids are known to contribute to various renal disorders; therefore, expanding the availability of analytical methodology to detect these compounds is important in order to assess the quality of Chinese herbal medicines in which they can be found. Twelve medicinal herbal samples were procured from various sources and extracted in duplicate prior to a "fingerprint" analysis using conventional HPLC-DAD. Multivariate analysis was performed on the entire chromatographed fingerprints. The resulting output was a partial least-square discriminant analysis model, which was able to evaluate the potential presence of aristolochic acids I and II as well as providing an individual herbal "fingerprint". The results of this study provide evidence that the presence of aristolochic acids contained within certain herbal extractions could be detected using a simple method, although some limitations apply to this method for quality control, since newly detected samples for aristolochic acid (positives) will need further confirmation with purity checks or MS hyphenation.
authors.affiliation: Pharmaceutical Sciences Research Division, King's College London, London, UK.
authors.forename: James
authors.initials: J
authors.lastname: Heaton
authors.forename: Luke
authors.initials: L
authors.lastname: Whiley
authors.forename: Yingzi
authors.initials: Y
authors.lastname: Hong
authors.forename: Chinnu Mary
authors.initials: CM
authors.lastname: Sebastian
authors.forename: Norman William
authors.initials: NW
authors.lastname: Smith
authors.forename: Cristina
authors.initials: C
authors.lastname: Legido-Quigley
mesh_terms: D034341:Aristolochic Acids / Q000032:analysis* / Q000633:toxicity; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000633:toxicity
chemical_list: D034341:Aristolochic Acids; D004365:Drugs, Chinese Herbal