title: Molecular cloning, sequencing, and gene expression analysis of tributyltin-binding protein type 1 in Japanese medaka fish, Oryzias latipes.
abstract: The full-length cDNA sequence of tributyltin-binding protein type 1 in Japanese medaka (Oryzias latipes) (Olat.TBT-bp1) was determined by means of rapid amplification of cDNA ends (RACE) of liver tissue. Analysis of the structure of the gene encoding Olat.TBT-bp1 revealed that the exonintron organization of this gene corresponds to that of the genes encoding lipocalin superfamily proteins, suggesting that Olat.TBT-bp1 can be categorized as a member of the lipocalin superfamily, which may play an important role in transportation, detoxification, and excretion of xenobiotic compounds. Reverse transcription - PCR revealed that Olat.TBT-bp1 was expressed mainly in the liver, and upregulation of its expression was detected 1, 2, and 4 weeks post hatching. Relative expression of the Olat.TBT-bp1 gene was significantly downregulated, compared with that in the solvent control, by exposure to tributyltin at 0.01 mg/l or triclosan at 1.7 mg/l. Further studies on Olat.TBT-bp1 expression in conjunction with other biochemical and physiological toxicities in response to chemical exposures are needed to increase our understanding and information of TBT-bps mechanisms and as molecular biomarkers of chemical exposures. The role of Olat.TBT-bp1 in xenobiotic detoxification and/or excretion needs more investigations.
authors.affiliation: Laboratory of Marine Environmental Science, Faculty of Agriculture, Kyushu University, Hakozaki 6-10-1, Higashi-ku, Fukuoka 812-8581, Japan.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Nassef
authors.forename: Yoko
authors.initials: Y
authors.lastname: Kato-Unoki
authors.forename: Tomohisa
authors.initials: T
authors.lastname: Furuta
authors.forename: Kei
authors.initials: K
authors.lastname: Nakayama
authors.forename: Hina
authors.initials: H
authors.lastname: Satone
authors.forename: Yohei
authors.initials: Y
authors.lastname: Shimasaki
authors.forename: Tsuneo
authors.initials: T
authors.lastname: Honjo
authors.forename: Yuji
authors.initials: Y
authors.lastname: Oshima
mesh_terms: D000818:Animals; D001483:Base Sequence; D002220:Carbamazepine / Q000633:toxicity; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D004008:Diclofenac / Q000633:toxicity; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D054834:Lipocalins / Q000737:chemistry / Q000235:genetics; D008099:Liver / Q000502:physiology; D008969:Molecular Sequence Data; D009990:Oryzias / Q000235:genetics* / Q000378:metabolism*; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D014220:Trialkyltin Compounds / Q000378:metabolism*; D014260:Triclosan / Q000633:toxicity
chemical_list: D002352:Carrier Proteins; D029941:Fish Proteins; D054834:Lipocalins; D014220:Trialkyltin Compounds; D004008:Diclofenac; D002220:Carbamazepine; D014260:Triclosan; C011559:tributyltin