title: The effects of aging on the central nervous system steroid profiles and myelin basic protein in rats.
abstract: The aim of this study was to investigate the effects of aging on the central nervous system steroid and myelin basic protein (MBP) profiles. Forty-seven male Sprague-Dawley rats (newborn, 1, 6, 12 and 24-monthsold) were studied. Tissues were obtained from the cerebellum and parietal, frontal, temporal cortex of the central nervous system of the rats for steroid extraction. The estradiol, progesteron, testosterone and dehydroepiandrosterone (DHEA) levels were measured by Enzyme Linked Immunosorbent Assay (ELISA). The average levels of estradiol (pg/g), progesteron (ng/g), DHEA (ng/g) and testosterone (ng/g) in the brain tissues were respectively 24.29, 4.59, 0.27, 0.92 in the newborn-rats; 4.18 ± 1.10, 1.54 ± 0.30, 0.28 ± 0.01, 0.57 ± 0.10 in the 1 month-old-rats; 11.02 ± 1.10, 2.96 ± 0.30, 0.27 ± 0.01, 0.61 ± 0.10 in the 6 month-old-rats; 15.80 ± 1.10, 4.80 ± 0.30, 0.28 ± 0.10, 0.67 ± 0.10 in the 12 monthold- rats; 20.07 ± 1.10, 4.12 ± 0.30, 0.28 ± 0.01, 0.55±0.10 in the 24 month-old-rats. The myelin basic protein levels were determined by immunohistochemical staining and an elevation was observed in conjunction with the aging process. The results of the study indicate that the alterations in MBP, DHEA, progesterone, testosterone and estrodiol concentrations in the central nervous system of the rats during aging can be considered fundamental for future animal and human studies.
authors.affiliation: Department of Biochemistry, Faculty of Veterinary Medicine, University of Ondokuz Mayis, 55139, Samsun, Turkey. gciftci@omu.edu.tr
authors.forename: Gulay
authors.initials: G
authors.lastname: Ciftci
authors.forename: Gul Fatma
authors.initials: GF
authors.lastname: Yarim
authors.forename: Murat
authors.initials: M
authors.lastname: Yarim
authors.forename: Mehmet Onder
authors.initials: MO
authors.lastname: Karayigit
authors.forename: Sena
authors.initials: S
authors.lastname: Cenesiz
authors.forename: Cevat
authors.initials: C
authors.lastname: Nisbet
authors.forename: Murat Erdem
authors.initials: ME
authors.lastname: Gultiken
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D002490:Central Nervous System / Q000378:metabolism*; D003687:Dehydroepiandrosterone / Q000378:metabolism; D004958:Estradiol / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D004676:Myelin Basic Protein / Q000378:metabolism*; D011374:Progesterone / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013256:Steroids / Q000378:metabolism*; D013739:Testosterone / Q000378:metabolism
chemical_list: D004676:Myelin Basic Protein; D013256:Steroids; D013739:Testosterone; D003687:Dehydroepiandrosterone; D011374:Progesterone; D004958:Estradiol