title: Complex ovarian defects lead to infertility in Etv5-/- female mice.
abstract: Etv5 is a member of the Etv4 subfamily of Ets transcription factors. In female mice, Etv5 was previously shown to be expressed in the mouse ovary. In this work, we show that Etv5-/- female mice are infertile due to a complex reproductive phenotype. Defects in the ovarian tissue architecture were noted as early as 2 weeks of age in Etv5-/- mice. Adult Etv5-/- female mice show decreased ovulation and no interest in mating even after gonadotrophin treatment. Histological abnormalities were also noted in Etv5-/- ovaries. Injection of 17β-estradiol to gonadotrophin-treated Etv5-/- mice significantly increased ovulation, mating and fertilization rates. However, 2-cell embryos of Etv5-/- females show compromised development, suggesting a role for Etv5 in the developmental competence of embryos. Expression of aromatase (CYP11a1), 17α-hydroxylase/17,20 lyase/17,20 desmolase (CYP17a1), side-chain-cleaving enzyme (CYP19a1) and luteinizing hormone/choriogonadotropin receptor mRNAs was not significantly altered in Etv5-/- ovaries. Collectively, our results suggest that Etv5 is important for the developmental competence of germ cells and the regulation of responses to steroid hormones in female mice.
authors.affiliation: Department of Biomedical Science and Technology, RCTC, IBST, Konkuk University, 1 Hwayang-dong, Kwangjin-gu, Seoul 143-701, Republic of Korea.
authors.forename: Jinwon
authors.initials: J
authors.lastname: Eo
authors.forename: Hyejin
authors.initials: H
authors.lastname: Shin
authors.forename: Sojung
authors.initials: S
authors.lastname: Kwon
authors.forename: Haengseok
authors.initials: H
authors.lastname: Song
authors.forename: Kenneth M
authors.initials: KM
authors.lastname: Murphy
authors.forename: Jade Hyunjung
authors.initials: JH
authors.lastname: Lim
mesh_terms: D000818:Animals; D001141:Aromatase / Q000235:genetics / Q000378:metabolism; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000235:genetics / Q000378:metabolism; D006063:Chorionic Gonadotropin / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D005306:Fertilization / Q000502:physiology; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D010053:Ovary / Q000002:abnormalities* / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D010060:Ovulation / Q000187:drug effects / Q000502:physiology; D050783:Proto-Oncogene Proteins c-ets / Q000235:genetics / Q000378:metabolism; D011974:Receptors, LH / Q000235:genetics / Q000378:metabolism; D012726:Sexual Behavior, Animal / Q000502:physiology; D013254:Steroid 17-alpha-Hydroxylase / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D006063:Chorionic Gonadotropin; D004268:DNA-Binding Proteins; C540571:Etv4 protein, mouse; C513242:Etv5 protein, mouse; D050783:Proto-Oncogene Proteins c-ets; D011974:Receptors, LH; D014157:Transcription Factors; D004958:Estradiol; D001141:Aromatase; D013254:Steroid 17-alpha-Hydroxylase; D002786:Cholesterol Side-Chain Cleavage Enzyme