title: Pnlip encoding pancreatic lipase is a possible candidate for intramuscular fat accumulation QTL in OLETF rat.
abstract: A quantitative trait locus (QTL) responsible for intramuscular fat accumulation in Musculus longissimus of Otsuka Long-Evans Tokushima Fatty (OLETF) rat, Imfm, was previously mapped to the approximately 10-cM genomic region between D1Rat166 and D1Rat90 on chromosome 1 using Imfm congenic strain. In this study, we refined the Imfm region to a approximately 2.3-cM genomic region between D1Rat225 and D1Rat90, using 12 informative recombinants selected from 176 (Imfm congenic x F344) F, x Imfm congenic backcross progenies. Among 46 genes located within the approximately 2.3-cM region, pancreatic lipase gene, Pnlip, that is a possible candidate for obesity QTL, Nidd6/of, was thought to be the most prominent and physiologically relevant positional candidate for the Imfm QTL. It was previously showed that Pnlip possesses an OLETF allele-specific increase of mRNA levels in the pancreas, and that the OLETF allele is longer in variable number of tandem repeat (VNTR) within the 5'-flanking region than normal alleles. We found complete cosegregation of the Imfm QTL with Pnlip VNTR in the 12 informative recombinants, suggesting that Pnlip is also a possible candidate for the Imfm QTL.
authors.affiliation: Department of Food Sciences, Shizuoka Eiwa Gakuin University Junior College, Ikeda, Shizuoka, Shizuoka 422-8005, Japan.
authors.forename: Youji
authors.initials: Y
authors.lastname: Muramatsu
authors.forename: Takahisa
authors.initials: T
authors.lastname: Yamada
authors.forename: Yukio
authors.initials: Y
authors.lastname: Taniguchi
authors.forename: Tomoe
authors.initials: T
authors.lastname: Ogino
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kose
authors.forename: Kozo
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Sasaki
mesh_terms: D050154:Adiposity / Q000235:genetics*; D000818:Animals; D020296:Animals, Congenic; D005260:Female; D007178:Inbreeding; D008049:Lipase / Q000235:genetics*; D008297:Male; D018482:Muscle, Skeletal / Q000033:anatomy & histology* / Q000201:enzymology; D040641:Quantitative Trait Loci; D051381:Rats; D011916:Rats, Inbred F344; D020334:Rats, Inbred OLETF / Q000033:anatomy & histology* / Q000235:genetics* / Q000378:metabolism
chemical_list: D008049:Lipase