title: [Expression and distribution of transforming growth factor β3 in the mouse tooth germ during development after advanced bell stage].
abstract: OBJECTIVE
  To observe the expression and distribution of transforming growth factor β3 (TGF-β3) in the mouse tooth germ after advanced bell stage, and to discuss the role of TGF-β3 during the development of tooth germs.
  
  
  METHODS
  BALB/C's mouse tooth germs at 4, 11, and 18 days postnatal (4dpn,11dpn,and 18dpn) were collected and processed for routine fixation, decalcification, embedding, and slicing. The expression of TGF-β3 was detected by immunohistochemisty.
  
  
  RESULTS
  As to 4dpn tooth germ: Positive expression of TGF-β3 was found in enameloblasts, odontoblasts, ambitus of dental pupilla, with weak positive expression in the intermedial of dental papilla. As to 11dpn tooth germ: Positive expression was seen in enameloblasts, with negative expression in odontoblasts and dental papilla. As to 18dpn tooth, positive expression of TGF-β3 was showed in the vessel wall and its surrounding, with negative expression in other areas.
  
  
  CONCLUSIONS
  The distribution of TGF-β3 expression showed a time-space characteristic during the mouse tooth germ development after advanced bell stage, which may exert a regulatory effect on tooth development and this effect is gradually getting weak with the development of tooth germs.
authors.affiliation: Department of Stomatology, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Guoying
authors.initials: G
authors.lastname: Que
authors.forename: Heng
authors.initials: H
authors.lastname: Li
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Zou
authors.forename: Ying
authors.initials: Y
authors.lastname: Wu
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D008963:Molar / Q000378:metabolism; D014018:Tissue Distribution; D014083:Tooth Germ / Q000254:growth & development / Q000378:metabolism*; D053782:Transforming Growth Factor beta3 / Q000378:metabolism*
chemical_list: C509045:Tgfb3 protein, mouse; D053782:Transforming Growth Factor beta3