title: Recombinant outer membrane protein A (OmpA) of Edwardsiella tarda, a potential vaccine candidate for fish, common carp.
abstract: Outer membrane protein A (OmpA) is a component of the outer membrane of Edwardsiella tarda and is wildly distributed in Enterobacteriaceae family. The gene encoding the OmpA protein was cloned from E. tarda and expressed in Escherichia coli M15 cells. The recombinant OmpA protein containing His(6) residues was estimated to have a molecular weight of ~38kDa. In Western blot the native protein showed expression at ~36kDa molecular weight which was within the range of major outer membrane proteins (36-44kDa) observed in this study. All E. tarda isolates tested harbored the ompA gene and the antibody raised to this protein was seen to cross react with other Gram negative bacteria. The OmpA protein characterized in this study was observed to be highly immunogenic in both rabbit and fish. In Enzyme linked immunosorbent assay, rabbit antisera showed an antibody titer of 1: 128,000. Common carp vaccinated with recombinant OmpA protein elicited high antibody production and immunized fish showed a relative percentage survival of 54.3 on challenge.
authors.affiliation: UNESCO-MIRCEN for Marine Biotechnology, Department of Fishery Microbiology, Karnataka Veterinary, Animal & Fisheries Sciences University, College of Fisheries, Mangalore, India.
authors.forename: Biswajit
authors.initials: B
authors.lastname: Maiti
authors.forename: Mahesh
authors.initials: M
authors.lastname: Shetty
authors.forename: Malathi
authors.initials: M
authors.lastname: Shekar
authors.forename: Iddya
authors.initials: I
authors.lastname: Karunasagar
authors.forename: Indrani
authors.initials: I
authors.lastname: Karunasagar
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology*; D001428:Bacterial Vaccines / Q000235:genetics / Q000276:immunology*; D002347:Carps; D020609:Edwardsiella tarda / Q000235:genetics / Q000276:immunology*; D004756:Enterobacteriaceae Infections / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D005393:Fish Diseases / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D011817:Rabbits; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D011994:Recombinant Proteins; C078167:OMPA outer membrane proteins