title: Identification of Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa in blood cultures: a multicenter performance evaluation of a three-color peptide nucleic acid fluorescence in situ hybridization assay.
abstract: A multicenter evaluation was undertaken to evaluate the performance of a new three-color peptide nucleic acid fluorescence in situ hybridization assay that identifies isolates directly from blood cultures positive for Gram-negative bacilli (GNB). The assay correctly identified 100% (186/186) of the Escherichia coli isolates, 99.1% (109/110) of the Klebsiella pneumoniae isolates, and 95.8% (46/48) of the Pseudomonas aeruginosa isolates in this study. Negative assay results were correctly obtained for 162 of 165 other GNB (specificity, 98.2%).
authors.affiliation: Columbia University Medical Center, NewYork-Presbyterian Hospital, New York, New York, USA.
authors.forename: Phyllis
authors.initials: P
authors.lastname: Della-Latta
authors.forename: Hossein
authors.initials: H
authors.lastname: Salimnia
authors.forename: Theresa
authors.initials: T
authors.lastname: Painter
authors.forename: Fann
authors.initials: F
authors.lastname: Wu
authors.forename: Gary W
authors.initials: GW
authors.lastname: Procop
authors.forename: Deborah A
authors.initials: DA
authors.lastname: Wilson
authors.forename: Wendy
authors.initials: W
authors.lastname: Gillespie
authors.forename: Alayna
authors.initials: A
authors.lastname: Mender
authors.forename: Benjamin S
authors.initials: BS
authors.lastname: Crystal
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D001769:Blood / Q000382:microbiology*; D003116:Color; D004926:Escherichia coli / Q000302:isolation & purification*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007711:Klebsiella pneumoniae / Q000302:isolation & purification*; D020135:Peptide Nucleic Acids; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
chemical_list: D020135:Peptide Nucleic Acids