title: [The quality control of 4C-clone screening assay].
abstract: Circular chromosome conformation capture (4C) can be used to analyze the high-resolution interaction map of cis-regulatory elements in genome-wide studies. In this study, we optimized the condition of PCR with the mimical 4C sample in order to establish a specific and efficient assay, which was validated practically. This 4C-clone screening assay can be used as the quality control in the application of 4C assay.
authors.affiliation: Institute of Biotechnology, Academy of Military Medical Science, Beijing 100071, China. tianjing1980s@126.com
authors.forename: Jing
authors.initials: J
authors.lastname: Tian
authors.forename: Na
authors.initials: N
authors.lastname: Chen
authors.forename: Zhi-Hu
authors.initials: ZH
authors.lastname: Zhao
authors.forename: Hui-Peng
authors.initials: HP
authors.lastname: Chen
mesh_terms: D002874:Chromosome Mapping / Q000592:standards*; D002875:Chromosomes / Q000737:chemistry*; D003001:Cloning, Molecular; D006367:HeLa Cells; D006801:Humans; D009690:Nucleic Acid Conformation; D016133:Polymerase Chain Reaction / Q000379:methods; D011786:Quality Control; D055748:SOXB1 Transcription Factors / Q000235:genetics; D013696:Temperature
chemical_list: C527782:SOX2 protein, human; D055748:SOXB1 Transcription Factors