title: Cell specific peptide-conjugated polysaccharide nanogels for protein delivery.
abstract: A cell specific peptide (Arg-Gly-Asp; RGD)-modified nanogel was prepared and evaluated for its potential to act as a protein delivery carrier. A bovine serum albumin (BSA)/RGD-modified nanogel complex was efficiently internalized into cells through integrin-mediated endocytosis. Endosomal escape of the RGD-modified nanogel was observed after 24 h incubation. The nanogel proved useful for targeted protein delivery.
authors.affiliation: Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-surugadai, Chiyoda-ku, Tokyo 101-0062, Japan.
authors.forename: Asako
authors.initials: A
authors.lastname: Shimoda
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Sawada
authors.forename: Kazunari
authors.initials: K
authors.lastname: Akiyoshi
mesh_terms: D045744:Cell Line, Tumor; D002966:Clathrin / Q000494:pharmacology; D004705:Endocytosis; D006367:HeLa Cells; D006801:Humans; D016023:Integrins; D000080385:Nanogels; D009842:Oligopeptides / Q000138:chemical synthesis* / Q000378:metabolism; D010873:Pinocytosis; D011092:Polyethylene Glycols / Q000138:chemical synthesis* / Q000378:metabolism; D011094:Polyethyleneimine / Q000138:chemical synthesis* / Q000378:metabolism; D021381:Protein Transport; D011509:Proteoglycans / Q000138:chemical synthesis* / Q000378:metabolism
chemical_list: D002966:Clathrin; D016023:Integrins; D000080385:Nanogels; D009842:Oligopeptides; D011509:Proteoglycans; C413692:polyethylene glycol polyethyleneimine nanogel; C093216:polysaccharide peptide; D011092:Polyethylene Glycols; C047981:arginyl-glycyl-aspartic acid; D011094:Polyethyleneimine