title: Nano-talc stabilizes TNF-alpha m-RNA in human macrophages.
abstract: Particle size reduction of talc from micro- to nanoscale gradually enhanced its cytotoxicity however its inflammatory potential is still not explored. In the current study we observed increased TNF-alpha, IL-1beta and IL-6 mRNA levels in macrophages exposed to Nano-Talc (NT). Further, NT particles also showed constituent phosphorylation of both p38 and ERK1/2 pathway however JNK phosphorylation was transient. Pre-treatment of macrophages with p38 and ERK1/2 inhibitors either alone or in combination showed significant reduction in TNF-alpha mRNA stability, clearly suggesting their role in TNF-alpha mRNA stabilization and expression. Our observations clearly demonstrated the inflammatory potential of NT particles which might be at least partial and potential mechanism in talc mediated pathogenecity in the exposed population.
authors.affiliation: Nanomaterial Toxicology Group, Indian Institute of Toxicology Research (CSIR), P.O. Box 80, M. G. Marg, Lucknow 226001, India.
authors.forename: Mohd Imran
authors.initials: MI
authors.lastname: Khan
authors.forename: Amogh A
authors.initials: AA
authors.lastname: Sahasrabuddhe
authors.forename: Govil
authors.initials: G
authors.lastname: Patil
authors.forename: Mohd Javed
authors.initials: MJ
authors.lastname: Akhtar
authors.forename: Mohd
authors.initials: M
authors.lastname: Ashquin
authors.forename: Iqbal
authors.initials: I
authors.lastname: Ahmad
mesh_terms: D002478:Cells, Cultured; D006801:Humans; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism*; D049329:Nanostructures / Q000008:administration & dosage*; D012333:RNA, Messenger / Q000378:metabolism*; D013627:Talc / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D013627:Talc