title: Effects of rhamnolipid on the cellulase and xylanase in hydrolysis of wheat straw.
abstract: The effects of biosurfactant rhamnolipid (RL) and chemical surfactant Triton X-100 on the production of cellulases and xylanase from Penicillium expansum (P. expansum) in untreated, acid- and alkali-pretreated wheat straw submerged fermentations were studied, and the influences on the activity and stability of Cellulase R-10 were also investigated. The results showed that RL and Triton X-100 enhanced the activities of cellulases and xylanase to different extents and the stimulatory effects of RL were superior to those of Triton X-100. During the peak enzyme production phase, RL (60 RE mg/l) increased cellulases activities by 25.5-102.9%, in which the raise of the same enzyme in acid-pretreated straw broths was the most. It was found that the reducing sugars by hydrolyzing wheat straw with Cellulase R-100 were not visibly increased after adding RL. However, it distinctly protected Cellulase R-10 from degradation or inactivation, keeping the reducing sugars yield at about 17%.
authors.affiliation: Ministry of Agriculture Key Laboratory of Crop Nutrition and Fertilization, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors.forename: Hong-Yuan
authors.initials: HY
authors.lastname: Wang
authors.forename: Bing-Quan
authors.initials: BQ
authors.lastname: Fan
authors.forename: Chun-Hua
authors.initials: CH
authors.lastname: Li
authors.forename: Shuang
authors.initials: S
authors.lastname: Liu
authors.forename: Min
authors.initials: M
authors.lastname: Li
mesh_terms: D002241:Carbohydrates / Q000032:analysis; D002480:Cellulase / Q000187:drug effects / Q000378:metabolism*; D005285:Fermentation / Q000187:drug effects; D006017:Glycolipids / Q000494:pharmacology*; D006868:Hydrolysis; D008031:Lignin / Q000032:analysis; D010407:Penicillium / Q000201:enzymology; D012972:Sodium Hydroxide / Q000494:pharmacology; D013464:Sulfuric Acids / Q000494:pharmacology; D014908:Triticum / Q000187:drug effects / Q000378:metabolism*; D014866:Waste Products / Q000032:analysis*
chemical_list: D002241:Carbohydrates; D006017:Glycolipids; D013464:Sulfuric Acids; D014866:Waste Products; C418382:rhamnolipid; C036909:lignocellulose; D012972:Sodium Hydroxide; D008031:Lignin; D002480:Cellulase; C033158:sulfuric acid