title: Targeted electroporation of defined lateral ventricular walls: a novel and rapid method to study fate specification during postnatal forebrain neurogenesis.
abstract: BACKGROUND
  Postnatal olfactory bulb (OB) neurogenesis involves the generation of granule and periglomerular cells by neural stem cells (NSCs) located in the walls of the lateral ventricle (LV). Recent studies show that NSCs located in different regions of the LV give rise to different types of OB neurons. However, the molecular mechanisms governing neuronal specification remain largely unknown and new methods to approach these questions are needed.
  
  
  RESULTS
  In this study, we refine electroporation of the postnatal forebrain as a technique to perform precise and accurate delivery of transgenes to NSCs located in distinct walls of the LV in the mouse. Using this method, we confirm and expand previous studies showing that NSCs in distinct walls of the LV produce neurons that invade different layers of the OB. Fate mapping of the progeny of radial glial cells located in these distinct LV walls reveals their specification into defined subtypes of granule and periglomerular neurons.
  
  
  CONCLUSIONS
  Our results provide a baseline with which future studies aiming at investigating the role of factors in postnatal forebrain neuronal specification can be compared. Targeted electroporation of defined LV NSC populations will prove valuable to study the genetic factors involved in forebrain neuronal specification.
authors.affiliation: Brain Research Institute, University of Zürich/ETHZ, CH-8057 Zürich, Switzerland.
authors.forename: María E
authors.initials: ME
authors.lastname: Fernández
authors.forename: Simona
authors.initials: S
authors.lastname: Croce
authors.forename: Camille
authors.initials: C
authors.lastname: Boutin
authors.forename: Harold
authors.initials: H
authors.lastname: Cremer
authors.forename: Olivier
authors.initials: O
authors.lastname: Raineteau
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation / Q000235:genetics; D002465:Cell Movement / Q000235:genetics / Q000528:radiation effects; D004566:Electrodes; D018274:Electroporation / Q000295:instrumentation / Q000379:methods*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D020547:Lateral Ventricles / Q000166:cytology / Q000502:physiology*; D051379:Mice; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D058953:Neural Stem Cells / Q000502:physiology; D055495:Neurogenesis / Q000235:genetics / Q000502:physiology*; D009457:Neuroglia / Q000502:physiology; D009474:Neurons / Q000502:physiology*; D016548:Prosencephalon / Q000166:cytology* / Q000254:growth & development*; D014741:Video Recording
chemical_list: D009419:Nerve Tissue Proteins; D049452:Green Fluorescent Proteins