title: Magnetite/CdTe magnetic-fluorescent composite nanosystem for magnetic separation and bio-imaging.
abstract: A new synthesis protocol is described to obtain a CdTe decorated magnetite bifunctional nanosystem via dodecylamine (DDA) as cross linker. High resolution transmission electron microscopy (HRTEM), energy-dispersive x-ray spectroscopy (EDAX), vibrating sample magnetometry (VSM), Fourier transform infrared spectroscopy (FTIR), diffuse reflectance spectroscopy (DRS) and fluorescence microscopy are used to characterize the constitution, size, composition and physical properties of these superparamagnetic-fluorescent nanoparticles. These CdTe decorated magnetite nanoparticles were then functionalized with anti-epidermal growth factor receptor (EGFR) antibody to specifically target cells expressing this receptor. The EGFR is a transmembrane glycoprotein and is expressed on tumor cells from different tissue origins including human leukemic cell line Molt-4 cells. The magnetite-CdTe composite nanosystem is shown to perform excellently for specific selection, magnetic separation and fluorescent detection of EGFR positive Molt-4 cells from a mixed population. Flow cytometry and confocal laser scanning microscopy results show that this composite nanosystem has great potential in antibody functionalized magnetic separation and imaging of cells using cell surface receptor antibody.
authors.affiliation: National Chemical Laboratory, Council of Scientific and Industrial Research, Pune, India.
authors.forename: Anup
authors.initials: A
authors.lastname: Kale
authors.forename: Sonia
authors.initials: S
authors.lastname: Kale
authors.forename: Prasad
authors.initials: P
authors.lastname: Yadav
authors.forename: Haribhau
authors.initials: H
authors.lastname: Gholap
authors.forename: Renu
authors.initials: R
authors.lastname: Pasricha
authors.forename: J P
authors.initials: JP
authors.lastname: Jog
authors.forename: Benoit
authors.initials: B
authors.lastname: Lefez
authors.forename: Béatrice
authors.initials: B
authors.lastname: Hannoyer
authors.forename: Padma
authors.initials: P
authors.lastname: Shastry
authors.forename: Satishchandra
authors.initials: S
authors.lastname: Ogale
mesh_terms: D019187:Cadmium Compounds / Q000737:chemistry*; D045744:Cell Line, Tumor; D002470:Cell Survival; D003287:Contrast Media / Q000737:chemistry*; D066246:ErbB Receptors / Q000378:metabolism; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods*; D049449:Luminescence; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D018613:Microscopy, Confocal / Q000379:methods; D009369:Neoplasms / Q000175:diagnosis*; D017550:Spectroscopy, Fourier Transform Infrared; D013691:Tellurium / Q000737:chemistry*; D013818:Thermogravimetry; D014961:X-Ray Diffraction
chemical_list: D019187:Cadmium Compounds; D003287:Contrast Media; D058185:Magnetite Nanoparticles; D066246:ErbB Receptors; D013691:Tellurium; C028337:cadmium telluride