title: LPA5 is abundantly expressed by human mast cells and important for lysophosphatidic acid induced MIP-1β release.
abstract: BACKGROUND
  Lysophosphatidic acid (LPA) is a bioactive lipid inducing proliferation, differentiation as well as cytokine release by mast cells through G-protein coupled receptors. Recently GPR92/LPA5 was identified as an LPA receptor highly expressed by cells of the immune system, which prompted us to investigate its presence and influence on mast cells.
  
  
  RESULTS
  Transcript analysis using quantitative real-time PCR revealed that LPA5 is the most prevalent LPA-receptor in human mast cells. Reduction of LPA5 levels using shRNA reduced calcium flux and abolished MIP-1β release in response to LPA.
  
  
  CONCLUSIONS
  LPA5 is a bona fide LPA receptor on human mast cells responsible for the majority of LPA induced MIP-1β release.
authors.affiliation: Department of Medicine, Harvard Medical School, Boston, Massachusetts, United States of America. Anders.Lundequist@slu.se
authors.forename: Anders
authors.initials: A
authors.lastname: Lundequist
authors.forename: Joshua A
authors.initials: JA
authors.lastname: Boyce
mesh_terms: D002460:Cell Line; D054407:Chemokine CCL4 / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D020868:Gene Silencing / Q000187:drug effects; D006801:Humans; D008246:Lysophospholipids / Q000494:pharmacology*; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D049368:Receptors, Lysophosphatidic Acid / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects
chemical_list: C066507:CCL4 protein, human; D054407:Chemokine CCL4; C512992:LPAR5 protein, human; D008246:Lysophospholipids; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D049368:Receptors, Lysophosphatidic Acid; C032881:lysophosphatidic acid