title: An HflX-type GTPase from Sulfolobus solfataricus binds to the 50S ribosomal subunit in all nucleotide-bound states.
abstract: HflX GTPases are found in all three domains of life, the Bacteria, Archaea, and Eukarya. HflX from Escherichia coli has been shown to bind to the 50S ribosomal subunit in a nucleotide-dependent manner, and this interaction strongly stimulates its GTPase activity. We recently determined the structure of an HflX ortholog from the archaeon Sulfolobus solfataricus (SsoHflX). It revealed the presence of a novel HflX domain that might function in RNA binding and is linked to a canonical G domain. This domain arrangement is common to all archaeal, bacterial, and eukaryotic HflX GTPases. This paper shows that the archaeal SsoHflX, like its bacterial orthologs, binds to the 50S ribosomal subunit. This interaction does not depend on the presence of guanine nucleotides. The HflX domain is sufficient for ribosome interaction. Binding appears to be restricted to free 50S ribosomal subunits and does not occur with 70S ribosomes engaged in translation. The fingerprint (1)H-(15)N heteronuclear correlation nuclear magnetic resonance (NMR) spectrum of SsoHflX reveals a large number of well-resolved resonances that are broadened upon binding to the 50S ribosomal subunit. The GTPase activity of SsoHflX is stimulated by crude fractions of 50S ribosomal subunits, but this effect is lost with further high-salt purification of the 50S ribosomal subunits, suggesting that the stimulation depends on an extrinsic factor bound to the 50S ribosomal subunit. Our results reveal common properties but also marked differences between archaeal and bacterial HflX proteins.
authors.affiliation: Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, Netherlands. fabian.blombach@gmail.com
authors.forename: Fabian
authors.initials: F
authors.lastname: Blombach
authors.forename: Helene
authors.initials: H
authors.lastname: Launay
authors.forename: Violeta
authors.initials: V
authors.lastname: Zorraquino
authors.forename: Daan C
authors.initials: DC
authors.lastname: Swarts
authors.forename: Lisa D
authors.initials: LD
authors.lastname: Cabrita
authors.forename: Dario
authors.initials: D
authors.lastname: Benelli
authors.forename: John
authors.initials: J
authors.lastname: Christodoulou
authors.forename: Paola
authors.initials: P
authors.lastname: Londei
authors.forename: John
authors.initials: J
authors.lastname: van der Oost
mesh_terms: D020558:GTP Phosphohydrolases / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D009711:Nucleotides / Q000378:metabolism*; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D025941:Protein Interaction Mapping; D054748:Ribosome Subunits, Large, Archaeal / Q000378:metabolism*; D048229:Sulfolobus solfataricus / Q000201:enzymology*
chemical_list: D009711:Nucleotides; D020558:GTP Phosphohydrolases