title: A fusion protein composed of receptor binding domain of vascular endothelial growth factor-A and constant region fragment of antibody: angiogenesis antagonistic activity.
abstract: Vascular endothelial growth factor (VEGF) promotes the growth of solid tumor mainly via VEGF receptor-1 and receptor-2, which are expressed preferentially in proliferating endothelial cells. Therefore, a strategy for simultaneous blockage of both VEGF receptors may have a useful therapeutic effect in tumor growth. In this study, we utilized a fusion protein which is composed of receptor binding domain of VEGF-A (RBDV) and the constant region fragment (Fc) of a human immunoglobulin G1 (IgG1), to interfere with the growth of human umbilical vein endothelial cells (HUVECs) via VEGF receptors. The results showed that RBDV-IgG1 Fc was able to bind with both VEGF receptor-1 and receptor-2. In addition, RBDV-IgG1 Fc could decrease VEGF-induced proliferation and tube formation among HUVECs. Moreover, the cytotoxic test showed RBDV-IgG1 Fc could also enhance the cytotoxic activity of human natural killing cells. The data are suggesting that the fusion protein, RBDV-IgG1 Fc, may have potential as an angiogenesis antagonist for future tumor therapy.
authors.affiliation: Department of Orthopedics, Hualien Armed Forces Hospital, Hualien, 97144, Taiwan, ROC.
authors.forename: Feng-Jen
authors.initials: FJ
authors.lastname: Tseng
authors.forename: Yen-Ku
authors.initials: YK
authors.lastname: Liu
authors.forename: Yo-Shong
authors.initials: YS
authors.lastname: Chung
authors.forename: Yu-Ling
authors.initials: YL
authors.lastname: Lin
authors.forename: Chia-Hung
authors.initials: CH
authors.lastname: Chen
authors.forename: Wan-Yi
authors.initials: WY
authors.lastname: Wang
authors.forename: Yu-Cheng
authors.initials: YC
authors.lastname: Chen
authors.forename: Nu-Man
authors.initials: NM
authors.lastname: Tsai
authors.forename: Tian-Lu
authors.initials: TL
authors.lastname: Cheng
authors.forename: Ru-Yu
authors.initials: RY
authors.lastname: Pan
authors.forename: Tsung-Ming
authors.initials: TM
authors.lastname: Hu
authors.forename: Ru-Ping
authors.initials: RP
authors.lastname: Lee
authors.forename: Kuang-Wen
authors.initials: KW
authors.lastname: Liao