title: The use of multifunctional magnetic mesoporous core/shell heteronanostructures in a biomolecule separation system.
abstract: A multifunctional magnetic mesoporous core/shell heteronanostructure (designated as Fe(3)O(4)@NiSiO(3)) has been designed and constructed that combined the capacity of effective protein purification from protein mixture and selective low molecule weight (MW) biomolecule enrichment. The nanoparticle is composed by magnetite nanoparticle with immobilized metal ion surface and solid porous shell which presents a number of important features, such as controllable shell thickness, uniform pore size and excellent magnetism. By taking advantages of the high affinity of Ni(2+) on the shell surface toward His-tagged proteins and the fast response toward an assistant magnet, the heteronanoparticles can be applied to selectively bind to and magnetically separate of His-tagged proteins from a cell lysate of E. coli. Additionally, owing to the homogeneous 3D mesopores on the nickel silicate shell, the heteronanoparticles can selectively capture low MW biomolecules from complex mixture. Significantly, it is expected that this approach can be extended to other biomolecule separation and enrichment systems by changing the immobilized surface and the pore size.
authors.affiliation: State Key Laboratory of Rare Earth Resources Utilization and Laboratory of Chemical Biology, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Liu
authors.forename: Meng
authors.initials: M
authors.lastname: Li
authors.forename: Xinjian
authors.initials: X
authors.lastname: Yang
authors.forename: Meili
authors.initials: M
authors.lastname: Yin
authors.forename: Jinsong
authors.initials: J
authors.lastname: Ren
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Qu
mesh_terms: D004926:Escherichia coli / Q000737:chemistry; D029968:Escherichia coli Proteins / Q000302:isolation & purification; D006801:Humans; D007501:Iron / Q000737:chemistry; D058185:Magnetite Nanoparticles; D008422:Materials Testing; D009532:Nickel / Q000737:chemistry; D010316:Particle Size; D016062:Porosity; D012709:Serum Albumin / Q000302:isolation & purification; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D014465:Ultrasonics
chemical_list: D029968:Escherichia coli Proteins; D058185:Magnetite Nanoparticles; D012709:Serum Albumin; D009532:Nickel; D007501:Iron