title: In vitro interaction study of retinoic acid isomers with telmisartan and amlodipine by equilibrium dialysis method using UV spectroscopy.
abstract: The in vitro protein binding of retinoic acid isomers (isotretinoin and tretinoin) and the antihypertensive drugs (amlodipine and telmisartan) was studied by equilibrium dialysis method. In this study, free fraction of drugs and the % of binding of drugs in the mixture to bovine serum albumin (BSA) were calculated. The influence of retinoic acid isomers on the % of protein binding of telmisartan and amlodipine at physiological pH (7.4) and temperature (37±0.5°C) was also evaluated. The in vitro displacement interaction study of drugs telmisartan and amlodipine on retinoic acid isomers and also interaction of retinoic acid isomers on telmisartan and amlodipine were carried out.
authors.affiliation: Department of Pharmaceutical Analysis, College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences, 395 Sarojini Naidu Road, Sidhapudur, Coimbatore 641 044, Tamil Nadu, India. susheeljv@yahoo.com
authors.forename: Susheel John
authors.initials: SJ
authors.lastname: Varghese
authors.forename: Sojimol K
authors.initials: SK
authors.lastname: Johny
authors.forename: David
authors.initials: D
authors.lastname: Paul
authors.forename: Thengungal Kochupappy
authors.initials: TK
authors.lastname: Ravi
mesh_terms: D017311:Amlodipine / Q000378:metabolism* / Q000494:pharmacology; D000959:Antihypertensive Agents / Q000378:metabolism* / Q000494:pharmacology; D001562:Benzimidazoles / Q000378:metabolism* / Q000494:pharmacology; D001565:Benzoates / Q000378:metabolism* / Q000494:pharmacology; D003956:Dialysis; D004347:Drug Interactions; D007536:Isomerism; D015474:Isotretinoin / Q000378:metabolism* / Q000494:pharmacology; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D000077333:Telmisartan; D014212:Tretinoin / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D000959:Antihypertensive Agents; D001562:Benzimidazoles; D001565:Benzoates; D017311:Amlodipine; D012710:Serum Albumin, Bovine; D014212:Tretinoin; D015474:Isotretinoin; D000077333:Telmisartan