title: CD36 is involved in lycopene and lutein uptake by adipocytes and adipose tissue cultures.
abstract: METHODS
  Carotenoids are mainly stored in adipose tissue. However, nothing is known regarding the uptake of carotenoids by adipocytes. Thus, our study explored the mechanism by which lycopene and lutein, two major human plasma carotenoids, are transported.
  
  
  RESULTS
  CD36 was a putative candidate for this uptake, 3T3-L1 cells were treated with sulfosuccinimidyl oleate, a CD36-specific inhibitor. sulfosuccinimidyl oleate-treated cells showed a significant decrease in both lycopene and lutein uptake as compared to control cells. Their uptake was also decreased by partial inhibition of CD36 expression using siRNA, whereas the overexpression of CD36 in Cos-1 cells increased their uptake. Finally, the effect of CD36 on carotenoid uptake was confirmed ex vivo in cultures of adipose tissue explants from CD36(-/-) mice, which exhibited reduced carotenoid uptake as compared to wild-type mice explants.
  
  
  CONCLUSIONS
  For the first time, we report the involvement of a transporter, CD36, in carotenoid uptake by adipocytes and adipose tissue.
authors.affiliation: INRA, UMR1260 "Nutriments Lipidiques et Prévention des Maladies Métaboliques", Marseille, France.
authors.forename: Myriam
authors.initials: M
authors.lastname: Moussa
authors.forename: Erwan
authors.initials: E
authors.lastname: Gouranton
authors.forename: Béatrice
authors.initials: B
authors.lastname: Gleize
authors.forename: Claire El
authors.initials: CE
authors.lastname: Yazidi
authors.forename: Isabelle
authors.initials: I
authors.lastname: Niot
authors.forename: Philippe
authors.initials: P
authors.lastname: Besnard
authors.forename: Patrick
authors.initials: P
authors.lastname: Borel
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Landrier
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism; D052436:Adipose Tissue, White / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D018955:CD36 Antigens / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D019556:COS Cells; D002338:Carotenoids / Q000378:metabolism*; D002522:Chlorocebus aethiops; D006801:Humans; D014975:Lutein / Q000378:metabolism*; D000077276:Lycopene; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009829:Oleic Acids / Q000494:pharmacology; D009924:Organ Culture Techniques; D034622:RNA Interference; D034741:RNA, Small Interfering; D013388:Succinimides / Q000494:pharmacology
chemical_list: D018955:CD36 Antigens; D009829:Oleic Acids; D034741:RNA, Small Interfering; D013388:Succinimides; C120556:sulfo-N-succinimidyl oleate; D002338:Carotenoids; D000077276:Lycopene; D014975:Lutein