title: Evaluation of cytotoxicity of new trans-palladium(II) complex in human cells in vitro.
abstract: Studies of cytotoxicity allow to elucidate the mechanisms by which chemical compounds influence cells and tissues. On the basis of the structural analogy between platinum(II) and palladium(II) complexes, a variety of studies on palladium(II) compounds as potential anticancer drugs have been carried out (1, 2). The cytotoxicity was evaluated by MTT assay. Abilities of trans-palladium(II) complex containing diethyl (pyridin-2-ylmethyl)phosphates as non-leaving ligands (trans-[PdCl2(2-pmOpe 2)]) to induce apoptosis and necrosis in normal lymphocytes, A549 cells and HT29 cell lines were performed by use of fluorochrome staining. The obtained results revealed, that the new trans-palladium(II) complex was more cytotoxic against A549 and HT29 tumor cells than on the normal lymphocytes in vitro. The novel complex induces apoptosis in all tested cells, but in lymphocytes to a lesser degree. The compound tested also induced significant amounts of necrotic cells, which exceeded the level of apoptotic cell fractions. The results demonstrate that the trans-Pd(II) complex showed substantial cytotoxic activity against A549 and HT29 tumor cells and indicate that the new trans-palladium(II) complex effectively inhibited cancer cells growth.
authors.affiliation: Laboratory of Cytogenetics, University of Lodz, 12/16 Banacha St., 90-237 Łódź, Poland. renkon@biol.uni.lodz.pl
authors.forename: Renata
authors.initials: R
authors.lastname: Kontek
authors.forename: Ksenia
authors.initials: K
authors.lastname: Matławska-Wasowska
authors.forename: Urszula
authors.initials: U
authors.lastname: Kalinowska-Lis
authors.forename: Bogdan
authors.initials: B
authors.lastname: Kontek
authors.forename: Justyn
authors.initials: J
authors.lastname: Ochocki
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology* / Q000633:toxicity; D017209:Apoptosis / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D019073:HT29 Cells; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects; D009336:Necrosis; D009369:Neoplasms / Q000473:pathology*; D010755:Organophosphates / Q000494:pharmacology* / Q000633:toxicity; D010165:Palladium / Q000494:pharmacology* / Q000633:toxicity
chemical_list: D000970:Antineoplastic Agents; D010755:Organophosphates; D010165:Palladium; C008756:palladium chloride