title: Chromium oxide nano-particles induce stress in bacteria: probing cell viability.
abstract: In this study, viability of an environmentally relevant bacterium, Escherichia coli exposed to 0-100 microg/mL chromium oxide nanoparticles (Cr2O3, Nps) for 120 min in Luria Bertani broth was evaluated by Propidium monoazide (PMA) assisted Q-PCR and standard plate count (SPC) method. Viable count for E. coli grown in Cr2O3, Nps amended medium was more by PMA assisted Q-PCR than SPC. Thus, the observations made in this study suggest that the inclusion of PMA assisted Q-PCR for viability assessment in Nps toxicity studies will provide the real count for the viable cells comprising of both viable and viable but not culturable (VBNC) cells.
authors.affiliation: Environmental Toxicology Group, Indian Institute of Toxicology Research (CSIR), M. G. Marg, P.O. Box 80, Lucknow 226001, India.
authors.forename: Gulshan
authors.initials: G
authors.lastname: Singh
authors.forename: Poornima
authors.initials: P
authors.lastname: Vajpayee
authors.forename: Imrana
authors.initials: I
authors.lastname: Khatoon
authors.forename: Anurag
authors.initials: A
authors.lastname: Jyoti
authors.forename: Alok
authors.initials: A
authors.lastname: Dhawan
authors.forename: K C
authors.initials: KC
authors.lastname: Gupta
authors.forename: Rishi
authors.initials: R
authors.lastname: Shanker
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D017608:Chromium Compounds / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000187:drug effects* / Q000502:physiology*; D053768:Metal Nanoparticles / Q000008:administration & dosage*; D018384:Oxidative Stress / Q000187:drug effects*
chemical_list: D017608:Chromium Compounds; C023600:chromic oxide