title: Cytochrome c oxidase subunit 1 barcode data of fish of the Nayband National Park in the Persian Gulf and analysis using meta-data flag several cryptic species.
abstract: We provide cytochrome c oxidase subunit 1 (COI) barcode sequences of fishes of the Nayband National Park, Persian Gulf, Iran. Industrial activities, ecological considerations and goals of The Fish Barcode of Life campaign make it crucial that fish species residing in the park be identified. To the best of our knowledge, this is the first report of barcoding data on fishes of the Persian Gulf. We examined 187 individuals representing 76 species, 56 genera and 32 families. The data flagged potentially cryptic species of Gerres filamentosus and Plectorhinchus schotaf. 16S rDNA data on these species are provided. Exclusion of these two potential cryptic species resulted in a mean COI intraspecific distance of 0.18%, and a mean inter- to intraspecific divergence ratio of 66.7. There was no overlap between maximum Kimura 2-parameter distances among conspecifics (1.66%) and minimum distance among congeneric species (6.19%). Barcodes shared among species were not observed. Neighbour-joining analysis showed that most species formed cohesive sequence units with little variation. Finally, the comparison of 16 selected species from this study with meta-data of conspecifics from Australia, India, China and South Africa revealed high interregion divergences and potential existence of six cryptic species. Pairwise interregional comparisons were more informative than global divergence assessments with regard to detection of cryptic variation. Our analysis exemplifies optimal use of the expanding barcode data now becoming available.
authors.affiliation: Department Biotechnology, College of Science, University of Tehran, No 13, Shafiie Alley, Qods St., Enghelab St., 14155-6455, Tehran, Iran.
authors.forename: Hosseinali
authors.initials: H
authors.lastname: Asgharian
authors.forename: Homayoun Hosseinzadeh
authors.initials: HH
authors.lastname: Sahafi
authors.forename: Aria Ashja
authors.initials: AA
authors.lastname: Ardalan
authors.forename: Shahrokh
authors.initials: S
authors.lastname: Shekarriz
authors.forename: Elahe
authors.initials: E
authors.lastname: Elahi
mesh_terms: D000818:Animals; D058893:DNA Barcoding, Taxonomic / Q000379:methods*; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics; D005399:Fishes / Q000145:classification* / Q000235:genetics*; D019156:Indian Ocean; D007492:Iran; D008969:Molecular Sequence Data; D010802:Phylogeny; D011110:Polymorphism, Genetic; D021122:Protein Subunits / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004272:DNA, Mitochondrial; D004275:DNA, Ribosomal; D021122:Protein Subunits; D012336:RNA, Ribosomal, 16S; D003576:Electron Transport Complex IV