title: Qualitative and quantitative analysis of glycated proteins in human plasma by glucose isotopic labeling with ¹³C6-reducing sugars.
abstract: Glucose is the predominant source of energy in cells. However, a chronic high glucose exposure of proteins modifies a number of biological pathways, known as glucotoxicity. Several studies have suggested that this impaired protein function is associated in part to protein glycation. However, despite the evidence of this glucotoxicity on tissues and cells, the exact mechanisms underlying the loss of protein function by glycation are not well understood. Strategies that will allow the discovery of the identity and function of the glycated plasma proteins generated by chronic hyperglycemia would be of a considerable help to further understand the underlying mechanisms implicated in protein dysfunction associated with glucotoxicity. The present chapter describes an innovative and interdisciplinary proteomics strategy to achieve a comprehensive identification and quantification of glycated proteins in plasma. This should provide new molecular insights into protein glycation mechanisms and identify new targets to improve the subsequent defective protein function.
authors.affiliation: Department of Structural Biology and Bioinformatics, Biomedical Proteomics Research Group, University Medical Centre, University of Geneva, Geneva, Switzerland.
authors.forename: Feliciano
authors.initials: F
authors.lastname: Priego-Capote
authors.forename: Maria
authors.initials: M
authors.lastname: Ramírez-Boo
authors.forename: Denis
authors.initials: D
authors.lastname: Hochstrasser
authors.forename: Jean-Charles
authors.initials: JC
authors.lastname: Sanchez
mesh_terms: D000595:Amino Acid Sequence; D001798:Blood Proteins / Q000032:analysis / Q000737:chemistry; D001800:Blood Specimen Collection; D001897:Boronic Acids / Q000737:chemistry; D050260:Carbohydrate Metabolism; D002247:Carbon Isotopes; D002846:Chromatography, Affinity; D005947:Glucose / Q000032:analysis / Q000378:metabolism*; D006020:Glycopeptides / Q000737:chemistry / Q000302:isolation & purification; D006023:Glycoproteins / Q000097:blood* / Q000737:chemistry; D006031:Glycosylation; D006801:Humans; D007553:Isotope Labeling / Q000379:methods*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D012697:Serine Endopeptidases / Q000378:metabolism
chemical_list: D001798:Blood Proteins; D001897:Boronic Acids; D002247:Carbon Isotopes; D006020:Glycopeptides; D006023:Glycoproteins; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase; D005947:Glucose