title: Epigenetic DNA methylation in the promoters of the Igf1 receptor and insulin receptor genes in db/db mice.
abstract: We have investigated promoter methylation of the Insr, Igf1 and Igf1r genes in skeletal and cardiac muscles of normal and diabetic db/db mice. No differences in Insr promoter methylation were found in the heart and skeletal muscles and no methylation was detected in the Igf1 promoter in skeletal muscle. In skeletal muscle, db/db males exhibited a 7.4-fold increase in Igf1r promoter methylation, which was accompanied by a 1.8-fold decrease in Igf1r mRNA levels, compared with controls. More than 50% of the detected methylation events were concentrated within an 18 bp sequence that includes one of the Sp1 binding sites. We conclude that the methylation level and pattern of the Igf1r promoter in skeletal muscle is related to gender and the diabetic state.
authors.affiliation: Department of Molecular Medicine and Surgery, Karolinska Institute, Stockholm, Sweden. Andrej.Nikosjkov@ki.se
authors.forename: Andrej
authors.initials: A
authors.lastname: Nikoshkov
authors.forename: Vivekananda
authors.initials: V
authors.lastname: Sunkari
authors.forename: Octavian
authors.initials: O
authors.lastname: Savu
authors.forename: Elisabete
authors.initials: E
authors.lastname: Forsberg
authors.forename: Sergiu-Bogdan
authors.initials: SB
authors.lastname: Catrina
authors.forename: Kerstin
authors.initials: K
authors.lastname: Brismar
mesh_terms: D000818:Animals; D001665:Binding Sites; D019175:DNA Methylation; D003921:Diabetes Mellitus, Experimental / Q000235:genetics; D044127:Epigenesis, Genetic; D005260:Female; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018482:Muscle, Skeletal / Q000378:metabolism; D011401:Promoter Regions, Genetic; D017526:Receptor, IGF Type 1 / Q000737:chemistry / Q000235:genetics*; D011972:Receptor, Insulin / Q000737:chemistry / Q000235:genetics*; D012737:Sex Factors
chemical_list: D017526:Receptor, IGF Type 1; D011972:Receptor, Insulin