title: Myelomonocytic THP-1 cells for in vitro testing of immunomodulatory properties of nanoparticles.
abstract: The use of nanoparticles for new therapeutic and diagnostics options represents a new risk for individuals exposed to such compounds. The myelomonocytic cell line THP-1 could be a useful alternative to human peripheral blood mononuclear cells (PBMC) to test for effects of drugs and compounds. Stimulation degree of cells can be monitored by measurement of neopterin and/or the kynurenine to tryptophan ratio. The method is robust and reproducible in the range of 0.1-1.0 microg/ml of LPS. However, compared to the PBMC assay it will not reveal any effect on the T-cell interaction.
authors.affiliation: Division of Biological Chemistry, Biocenter, Innsbruck Medical University, 6020 Innsbruck, Austria.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Schroecksnadel
authors.forename: Marcel
authors.initials: M
authors.lastname: Jenny
authors.forename: Dietmar
authors.initials: D
authors.lastname: Fuchs
mesh_terms: D002460:Cell Line; D016207:Cytokines / Q000276:immunology; D006801:Humans; D007113:Immunity, Innate / Q000187:drug effects* / Q000276:immunology*; D007155:Immunologic Factors / Q000494:pharmacology; D056747:Immunomodulation / Q000187:drug effects* / Q000276:immunology*; D007951:Leukemia, Myeloid / Q000276:immunology*; D008070:Lipopolysaccharides / Q000008:administration & dosage*; D053758:Nanoparticles / Q000008:administration & dosage*
chemical_list: D016207:Cytokines; D007155:Immunologic Factors; D008070:Lipopolysaccharides