title: Toward immunoassay chips: Facile immobilization of antibodies on cyclic olefin copolymer substrates through pre-activated polymer adlayers.
abstract: Our research efforts have focused on the surface modification and the potential as a biochip material of commercially available cyclic olefin copolymer (COC) substrates. For the surface modification of hydrophobic COC substrates we synthesized two types of amphiphilic polymers having three important functions: hydrophobic (dodecyl or benzyl) groups, serving to anchor the COC substrate; a PEG component, which acts as a repellent of non-specific biomolecules; and an NHS ester group for conjugation of biomolecules. Formation of the polymer adlayers on COC surface was confirmed using a contact angle analyzer. The anti-biofouling property of the polymer-coated COC surface was examined by measuring the extent of nonspecific adsorption of immunoglobulin G (IgG), resulting in a very low level of the protein adsorption compared to uncoated COC surfaces (control). In addition, antibodies, used as representative biomolecules for immunoassay, could be selectively immobilized on the polymer-coated COC surface. Sandwich immunoassay using anti-rabbit IgG immobilized COC surface showed linear response to rabbit IgG of model analyte over a range from 1 to 1000 ng/mL with detection limit of 1-10 ng/mL. We also fabricated a Lab-On-a Chip type of COC biochip, which could detect a cardiac marker protein, troponin I (TnI), with detection limit of 10 ng/mL.
authors.affiliation: Graduate Program of Medical System Engineering (GMSE), School of Life Sciences, Gwangju Institute of Science and Technology (GIST), 261 Cheomdan-gwagiro, Buk-gu, Gwangju 500-712, Republic of Korea.
authors.forename: Daekyung
authors.initials: D
authors.lastname: Sung
authors.affiliation: Biomed Research Team, BT Convergence Technology Research Department, IT Convergence Technology Research Laboratory, Electronics and Telecommunications Research Institute (ETRI), 138 Gajeongno, Yuseong-gu, Daejeon 305-700, Republic of Korea.
authors.forename: Dong Ho
authors.initials: DH
authors.lastname: Shin
authors.affiliation: Graduate Program of Medical System Engineering (GMSE), School of Life Sciences, Gwangju Institute of Science and Technology (GIST), 261 Cheomdan-gwagiro, Buk-gu, Gwangju 500-712, Republic of Korea; Cell Dynamics Research Center, School of Life Sciences, Gwangju Institute of Science and Technology (GIST), 261 Cheomdan-gwagiro, Buk-gu, Gwangju 500-712, Republic of Korea.
authors.forename: Sangyong
authors.initials: S
authors.lastname: Jon
mesh_terms: D000327:Adsorption; D000818:Animals; D055910:Antibodies, Immobilized / Q000737:chemistry*; D015374:Biosensing Techniques; D003516:Cycloparaffins / Q000737:chemistry; D007118:Immunoassay / Q000379:methods*; D007074:Immunoglobulin G / Q000737:chemistry / Q000302:isolation & purification*; D056656:Lab-On-A-Chip Devices; D011108:Polymers / Q000737:chemistry; D011817:Rabbits
chemical_list: D055910:Antibodies, Immobilized; D003516:Cycloparaffins; D007074:Immunoglobulin G; D011108:Polymers