title: Simultaneous quantification of four indole alkaloids in Catharanthus roseus cell line C20hi by UPLC-MS.
abstract: An ultra-performance liquid chromatography/mass spectrometry method to simultaneously quantify vindoline, catharanthine, serpentine and ajmalicine in Catharanthus roseus cell line C20hi is reported. Samples were extracted with 1% acetic acid, basified to pH 10 with ammonia, then extracted with ethyl acetate, dried, reconstituted with methanol-1% acetic acid water solution (1:1, v/v) and analyzed using an acetonitrile-0.1% formic acid gradient as the mobile phase. Detection was carried out by electrospray ionization mass spectrometry in the positive-ion mode with selective ion monitoring. The analysis of one sample was achieved in 6 min. The limits of detection were 0.46 - 0.70 ng/ml in cell samples, and 0.10 - 0.16 ng/ml in medium samples. The linearity of detection was over the wide range of 1.00 - 6250.0 ng/ml. Intra- and inter-day accuracies (recovery 88.0 - 111.8%) and precision (RSD 1.25 - 7.81%) showed the performance of the assay. This method provides a more sensitive and high-throughput technique to quantify the four alkaloids in large amount of samples, and will be helpful in high-production cultivar screening.
authors.affiliation: The Moe Key Laboratory of Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, PR China.
authors.forename: Lihong
authors.initials: L
authors.lastname: He
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.forename: Aizhen
authors.initials: A
authors.lastname: Xiong
authors.forename: Shujuan
authors.initials: S
authors.lastname: Zhao
authors.forename: Zhengtao
authors.initials: Z
authors.lastname: Wang
authors.forename: Zhibi
authors.initials: Z
authors.lastname: Hu
mesh_terms: D030008:Catharanthus / Q000166:cytology*; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D026121:Indole Alkaloids / Q000032:analysis* / Q000302:isolation & purification; D016014:Linear Models; D013058:Mass Spectrometry / Q000379:methods*; D013997:Time Factors
chemical_list: D026121:Indole Alkaloids