title: A label-free biosensor based on silver nanoparticles array for clinical detection of serum p53 in head and neck squamous cell carcinoma.
abstract: By using triangular silver (Ag) nanoparticle array, a localized surface plasmon resonance (LSPR) nanosensor was fabricated and shown to sense serum p53 protein in vitro, which is involved in head and neck squamous cell carcinoma (HNSCC). The nanosensor consists of a triangular Ag nanoparticles array with single particle dimension of 120 nm in-plane width and 45 nm out-of-plane height. When examined using LSPR nanobiosensor, the results indicated significant difference in LSPR shifts (Δλ(max)) between HNSCC patient and control. Although there is need for precise quantification and large-scale prospective, this report shows that the LSPR nanobiosensor provides a promising platform with attractive advantages for serological diagnosis or molecular diagnosis in tumor, such as HNSCC. This is the first clinical application of the LSPR nanosensor in HNSCC.
authors.affiliation: State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
authors.forename: Yingyi
authors.initials: Y
authors.lastname: Ma
authors.forename: Huan
authors.initials: H
authors.lastname: Yang
authors.forename: Yi
authors.initials: Y
authors.lastname: Ding
authors.forename: Xiangang
authors.initials: X
authors.lastname: Luo
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002277:Carcinoma / Q000097:blood / Q000175:diagnosis; D002294:Carcinoma, Squamous Cell; D006258:Head and Neck Neoplasms / Q000097:blood / Q000175:diagnosis; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D018307:Neoplasms, Squamous Cell / Q000097:blood / Q000175:diagnosis; D010446:Peptide Fragments / Q000097:blood*; D012680:Sensitivity and Specificity; D012834:Silver / Q000737:chemistry*; D000077195:Squamous Cell Carcinoma of Head and Neck; D020349:Surface Plasmon Resonance / Q000379:methods; D013499:Surface Properties; D016159:Tumor Suppressor Protein p53 / Q000097:blood*
chemical_list: D010446:Peptide Fragments; D016159:Tumor Suppressor Protein p53; C509415:p53 protein (325-355), human; D012834:Silver