title: An immunohistochemical study in cases with usual and unusual clinicopathological findings of canine visceral leishmaniosis.
abstract: The present study describes pathologic findings and immunohistochemical diagnosis of canine visceral leishmaniasis (CVL) in 22 dogs who died naturally in the Aegean region of Turkey. At necropsy, lymphadenomegaly, hepatosplenomegaly, hepatic, and nephrosclerotic lesions were conspicuous. Histopathologically, chronic inflammatory reactions of the spleen, lymph nodes, bone marrow, liver, and skin were marked findings. Cytological and histological examinations showed macrophages loaded with Leishmania amastigotes in these organs. Immunohistochemistry revealed that immunolabeling of amastigotes and/or parasite antigen, especially in the lymph nodes, spleen, bone marrow, liver, and skin, and occasionally, in the kidneys, intestines and lungs. Our laboratory results showed that immunohistochemistry should be included, along with cytological and histological examinations, in the diagnosis of CVL.
authors.affiliation: Department of Pathology, Faculty of Veterinary Medicine, Adnan Menderes University, 09016-Isikli, Aydin, Turkey. nihattoplu@hotmail.com
authors.forename: Nihat
authors.initials: N
authors.lastname: Toplu
authors.forename: Ahmet
authors.initials: A
authors.lastname: Aydogan
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000469:parasitology / Q000473:pathology; D004283:Dog Diseases / Q000175:diagnosis* / Q000453:epidemiology / Q000469:parasitology / Q000473:pathology; D004285:Dogs; D005260:Female; D007150:Immunohistochemistry / Q000379:methods* / Q000706:statistics & numerical data / Q000662:veterinary; D018314:Leishmania infantum / Q000502:physiology*; D007898:Leishmaniasis, Visceral / Q000175:diagnosis* / Q000453:epidemiology / Q000469:parasitology / Q000473:pathology / Q000662:veterinary; D008099:Liver / Q000469:parasitology / Q000473:pathology; D008198:Lymph Nodes / Q000469:parasitology / Q000473:pathology; D008264:Macrophages / Q000469:parasitology / Q000473:pathology; D008297:Male; D012867:Skin / Q000469:parasitology / Q000473:pathology; D013154:Spleen / Q000469:parasitology / Q000473:pathology; D014421:Turkey