title: Interaction of cancer cells with magnetic nanoparticles modified by methacrylamido-folic acid.
abstract: BACKGROUND
  Magnetic nanoparticles show great promise for use as tools in a wide variety of biomedical applications. The purpose of this study was to investigate the potential effects of methacrylamido-folic acid (Ma-Fol)-modified magnetic nanoparticles on 5RP7 (H-ras-transformed rat embryonic fibroblasts) and NIH/3T3 (normal mouse embryonic fibroblasts).
  
  
  METHODS
  The cytotoxicity and viability of 5RP7 and NIH/3T3 cells were detected. The percentage of cells undergoing apoptosis was analyzed by flow cytometry using Annexin V-fluorescein isothiocyanate staining. Nanoparticle internalization into 5RP7 and NIH/3T3 cells was visualized by transmission electron microscopy.
  
  
  CONCLUSIONS
  In this study, folic acid coupled to the surface of iron oxide for selective binding to cancer cells and immobilized the surfaces of magnetic nanoparticles. This complex improves cell internalization and targeting of cancer cells. We detected increased apoptosis using flow cytometry and transmission electron microscopy.
  
  
  RESULTS
  Folic acid modification of magnetic nanoparticles could be used to facilitate uptake to specific cancer cells for cancer therapy and diagnosis. Our results showed that the uptake of folic-acid modified nanoparticles by 5RP7 cancer cells was also much higher than that of 3T3 cells. This modification can be used for successful targeting of cancer cells expressing the folate receptor.
authors.affiliation: Department of Pharmaceutical Botanics, Faculty of Pharmacy, Anadolu University, Eskişehir, Turkey.
authors.forename: Nagehan
authors.initials: N
authors.lastname: Saltan
authors.forename: H Mehtap
authors.initials: HM
authors.lastname: Kutlu
authors.forename: Deniz
authors.initials: D
authors.lastname: Hür
authors.forename: Arzu
authors.initials: A
authors.lastname: Işcan
authors.forename: Ridvan
authors.initials: R
authors.lastname: Say
mesh_terms: D000178:Acrylamides / Q000737:chemistry; D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use; D017209:Apoptosis; D002471:Cell Transformation, Neoplastic / Q000737:chemistry / Q000378:metabolism; D016503:Drug Delivery Systems; D052203:Ferrosoferric Oxide / Q000737:chemistry; D005347:Fibroblasts / Q000378:metabolism; D005492:Folic Acid / Q000737:chemistry*; D011905:Genes, ras / Q000235:genetics; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D051379:Mice; D046529:Microscopy, Electron, Transmission; D041681:NIH 3T3 Cells; D010316:Particle Size; D051381:Rats
chemical_list: D000178:Acrylamides; D000970:Antineoplastic Agents; D058185:Magnetite Nanoparticles; D005492:Folic Acid; C045985:methacrylamide; D052203:Ferrosoferric Oxide