title: Nonlinear interferometric vibrational imaging for fast label-free visualization of molecular domains in skin.
abstract: The most prevalent molecular constituents of skin are spatially mapped by the use of nonlinear interferometric vibrational imaging, a coherent anti-Stokes Raman scattering (CARS)-based technique. Raman-like profiles over the range from 2,800 to 3,000 cm(-1) are acquired by means of completely suppressing the non-resonant background, allowing the generation of images based on the molecule-specific spectral profiles over the probed region with high spatial resolution. A simple algorithm that maps spectral content to color allows the visualization of histology in a manner analogous to that obtained with more conventional staining procedures (e.g., hematoxylin-eosin), but faster and with the benefit of having access to localized spectra, which could further enhance the potential for diagnosis of diseases, especially during the early stages of development.
authors.affiliation: Department of Electrical and Computer Engineering, Biophotonics Imaging Laboratory, Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, IL 61801, USA.
authors.forename: Wladimir A
authors.initials: WA
authors.lastname: Benalcazar
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Boppart
mesh_terms: D000465:Algorithms; D000818:Animals; D004867:Equipment Design; D007368:Interferometry / Q000295:instrumentation / Q000379:methods*; D012867:Skin / Q000737:chemistry / Q000648:ultrastructure*; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods*; D013552:Swine