title: Cytotoxicity and genotoxicity property of hydroxyapatite-mullite eluates.
abstract: Long-term biomedical applications of implant materials may cause osteolysis, aseptic losing and toxicity. Therefore, we investigated the cytotoxic and genotoxic potential of hydroxyapatite (HA) mullite eluates in L929 mouse fibroblast cells. The spark plasma sintered HA-20% mullite biocomposite (HA20M) were ground using mortar and pestle as well as ball milling. The cells were exposed for 6 h to varying concentrations (10, 25, 50, 75 and 100%) of the eluates of HA-20% mullite (87 nm), HA (171 nm) and mullite (154 nm). The scanning electron microscopy and MTT assay revealed the concentration dependent toxicity of H20M eluate at and above 50%. The analysis of the DNA damaging potential of HA, mullite and HA20M eluates using Comet assay demonstrated a significant DNA damage by HA20M which was largely related to the presence of mullite. The results collectively demonstrate the cytotoxic and genotoxic potential of HA20M eluate in L929 cells is dependent on particle size, concentration and composition.
authors.affiliation: Laboratory for Biomaterials, Department of Materials Science and Engineering, IIT Kanpur 208016, India.
authors.forename: Sushma
authors.initials: S
authors.lastname: Kalmodia
authors.forename: Vyom
authors.initials: V
authors.lastname: Sharma
authors.forename: Alok K
authors.initials: AK
authors.lastname: Pandey
authors.forename: Alok
authors.initials: A
authors.lastname: Dhawan
authors.forename: Bikramjit
authors.initials: B
authors.lastname: Basu
mesh_terms: D000538:Aluminum Silicates / Q000633:toxicity*; D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004249:DNA Damage; D005347:Fibroblasts / Q000187:drug effects* / Q000502:physiology*; D006882:Hydroxyapatites / Q000633:toxicity*; D053758:Nanoparticles / Q000633:toxicity*; D012996:Solutions
chemical_list: D000538:Aluminum Silicates; D006882:Hydroxyapatites; D012996:Solutions; C049037:aluminosilicate