title: Effects of age and dysfunction on human meibomian glands.
abstract: OBJECTIVE
  To identify age-related changes in human meibomian glands that may be associated with meibomian gland dysfunction (MGD).
  
  
  METHODS
  Excess eyelid tissue from 36 patients (age range, 18-95 years, 19 female, 17 male) who underwent canthoplasty procedures were used. Dermatologic history, age, and presence of MGD were recorded. Samples were frozen, sectioned, and stained with specific antibodies against peroxisome proliferator-activated receptor γ (PPARγ) to identify meibocyte differentiation, Ki67 nuclear antigen to identify cycling cells, and CD45 to identify inflammatory cell infiltration.
  
  
  RESULTS
  Staining for PPARγ showed cytoplasmic and nuclear localization in the 2 youngest subjects (ages, 18 and 44 years). Older individuals (>60 years) showed predominantly nuclear staining, with cytoplasmic staining limited to the basal acinar cells in 17 of 31 subjects. The number of Ki67 positively stained basal cells were significantly elevated in the younger compared with older subjects based on linear regression analysis (r(2) = 0.35; P < .001). There was also a significant correlation between MG expression grade and CD45 cell infiltration (r = 0.414; P = .05).
  
  
  CONCLUSIONS
  These results indicate that aging human meibomian glands show decreased meibocyte differentiation and cell cycling that is associated with the development of MGD. Findings also suggest that altered PPARγ signaling may lead to acinar atrophy and development of an age-related hyposecretory MGD.
  
  
  CONCLUSIONS
  Meibomian gland dysfunction and evaporative dry eye are common age-related eyelid disorders. Understanding the underlying mechanism of MGD may lead to the development of novel therapeutic strategies to treat this disease.
authors.affiliation: Gavin Herbert Eye Institute, Medical Center, University of California-Irvine, 101 The City Drive, Orange, CA 92868, USA.
authors.forename: Chyong Jy
authors.initials: CJ
authors.lastname: Nien
authors.forename: Salina
authors.initials: S
authors.lastname: Massei
authors.forename: Gloria
authors.initials: G
authors.lastname: Lin
authors.forename: Cameron
authors.initials: C
authors.lastname: Nabavi
authors.forename: Jeremiah
authors.initials: J
authors.lastname: Tao
authors.forename: Donald J
authors.initials: DJ
authors.lastname: Brown
authors.forename: Jerry R
authors.initials: JR
authors.lastname: Paugh
authors.forename: James V
authors.initials: JV
authors.lastname: Jester
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000502:physiology*; D002454:Cell Differentiation; D049109:Cell Proliferation; D005141:Eyelid Diseases / Q000378:metabolism* / Q000473:pathology; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D019394:Ki-67 Antigen / Q000378:metabolism; D017493:Leukocyte Common Antigens / Q000378:metabolism; D008297:Male; D008537:Meibomian Glands / Q000378:metabolism* / Q000473:pathology; D008875:Middle Aged; D047495:PPAR gamma / Q000378:metabolism
chemical_list: D019394:Ki-67 Antigen; D047495:PPAR gamma; D017493:Leukocyte Common Antigens; C516769:PTPRC protein, human