title: [Occurrence and the susceptibility to antimicrobial agents in Pseudomonas aeruginosa and Acinetobacter sp. at a tertiary hospital in southern Brazil].
abstract: BACKGROUND
  The main mechanism of emerging resistance in Pseudomonas aeruginosa and Acinetobacter sp. isolates is the production of metallo-β-lactamases (MβLs). MβLs are enzymes capable of hydrolyzing cephalosporins, penicillins and carbapenems, but not monobactams (aztreonam), which are often used as antimicrobial therapy to treat nosocomial infections.
  
  
  METHODS
  An observational descriptive and retrospective study was designed to assess the frequency of MβLs among strains of P. aeruginosa and Acinetobacter sp. obtained from a tertiary hospital in southern Brazil.
  
  
  RESULTS
  MβL production was observed in 77.6% (n = 173/223) for P. aeruginosa isolates and 22.4% (n = 50/223) of Acinetobacter sp. isolates. The Acinetobacter sp. isolates showed 92.8% sensitivity to amikacin and P. aeruginosa isolates showed 58.9% sensitivity to aztreonam.
  
  
  CONCLUSIONS
  The MβL indices determined confirm the global concern with this mechanism of resistance.
authors.affiliation: Instituto de Ciências Biológicas, Universidade de Passo Fundo, Passo Fundo, RS, Brasil.
authors.forename: Gabriele Mariani
authors.initials: GM
authors.lastname: Machado
authors.forename: Aldalise
authors.initials: A
authors.lastname: Lago
authors.forename: Sérgio Roberto Riccardi
authors.initials: SR
authors.lastname: Fuentefria
authors.forename: Daiane Bopp
authors.initials: DB
authors.lastname: Fuentefria
mesh_terms: D000150:Acinetobacter / Q000187:drug effects* / Q000201:enzymology / Q000302:isolation & purification; D000151:Acinetobacter Infections / Q000382:microbiology; D000293:Adolescent; D000328:Adult; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002648:Child; D002675:Child, Preschool; D003430:Cross-Sectional Studies; D052978:Disk Diffusion Antimicrobial Tests; D024901:Drug Resistance, Multiple, Bacterial; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D011552:Pseudomonas Infections / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000201:enzymology / Q000302:isolation & purification; D012189:Retrospective Studies; D055815:Young Adult; D001618:beta-Lactamases / Q000096:biosynthesis*
chemical_list: D000900:Anti-Bacterial Agents; D001618:beta-Lactamases