title: Variation assessment for in vitro permeabilities through Yucatan micropig skin.
abstract: The purpose of this study was to evaluate the variations in the in vitro Yucatan micropig (YMP) skin permeabilities of drugs and to clarify whether YMP skin can be used to predict human skin permeability. In vitro permeation studies of the three model drugs, nicorandil, isosorbide dinitrate and flurbiprofen, through YMP skin were performed using Franz-type diffusion cells. The permeation rates of the three model drugs were determined, and their variations were evaluated. The inter-individual variations in YMP skin permeability for the three model drugs were smaller than that in human skin permeability, and the permeation rates of the three model drugs through the YMP skin were approximately half that through human skin. In addition, the intra-individual variations in YMP skin permeability for nicorandil and flurbiprofen were much smaller than the inter-individual variations in YMP skin. The inter- and intra-regional variations in YMP skin permeability were very small. The markedly smaller variation in the permeability through YMP skin as compared with that through human skin indicated that in vitro permeation studies using YMP skin would be particularly useful for evaluating differences in the skin permeability of the three model drugs as well as for predicting human skin permeability.
authors.affiliation: Pharmacokinetics and Metabolism, Drug Safety and Pharmacokinetics Laboratories, Pharmaceutical Business, Taisho Pharmaceutical Co., Ltd., Yoshino-cho, Kita-ku, Saitama, Japan. hiroyuki.takeuchi@po.rd.taisho.co.jp
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Takeuchi
authors.forename: Shuichi
authors.initials: S
authors.lastname: Terasaka
authors.forename: Takanobu
authors.initials: T
authors.lastname: Sakurai
authors.forename: Atsushi
authors.initials: A
authors.lastname: Furuya
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Urano
authors.forename: Kenji
authors.initials: K
authors.lastname: Sugibayashi
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D001692:Biological Transport; D005480:Flurbiprofen / Q000493:pharmacokinetics; D006801:Humans; D007548:Isosorbide Dinitrate / Q000493:pharmacokinetics; D023421:Models, Animal; D020108:Nicorandil / Q000493:pharmacokinetics; D010539:Permeability; D010599:Pharmacokinetics; D012869:Skin Absorption; D013552:Swine; D013556:Swine, Miniature / Q000378:metabolism*
chemical_list: D020108:Nicorandil; D005480:Flurbiprofen; D007548:Isosorbide Dinitrate