title: Clovamide-rich extract from Trifolium pallidum reduces oxidative stress-induced damage to blood platelets and plasma.
abstract: Numerous plants (including clovers) have been widely used in folk medicine for the treatment of different disorders. This in vitro study was designed to examine the antioxidative effects of the clovamide-rich fraction, obtained from aerial parts of Trifolium pallidum, in the protection of blood platelets and plasma against the nitrative and oxidative damage, caused by peroxynitrite (ONOO(-)). Carbonyl groups and 3-nitrotyrosine in blood platelet and plasma proteins were determined by ELISA tests. Thiol groups level was estimated by using 5,5'-dithio-bis(2-nitro-benzoic acid, DTNB). Plasma lipid peroxidation was measured spectrophotometrically as the production of thiobarbituric acid reactive substances. The results from our work indicate that clovamide-rich T. pallidum extract may reveal the protective properties in the prevention against oxidative stress. The presence of clovamide-rich T. pallidum extract (12.5-100 μg/ml) partly inhibited ONOO(-)-mediated protein carbonylation and nitration. All the used concentrations of T. pallidum extract reduced lipid peroxidation in plasma. The antioxidative action of the tested extract in the protection of blood platelet lipids was less effective; the extract at the lowest final concentration (12.5 μg/ml) had no protective effect against lipid peroxidation. The present results indicate that the extract from T. pallidum is likely to be a source of compounds with the antioxidative properties, useful in the prevention against the oxidative stress-related diseases.
authors.affiliation: Department of General Biochemistry, University of Lodz, Pomorska 141/143, Lodz, Poland. joannak@biol.uni.lodz.pl
authors.forename: Joanna
authors.initials: J
authors.lastname: Kolodziejczyk
authors.forename: Beata
authors.initials: B
authors.lastname: Olas
authors.forename: Barbara
authors.initials: B
authors.lastname: Wachowicz
authors.forename: Barbara
authors.initials: B
authors.lastname: Szajwaj
authors.forename: Anna
authors.initials: A
authors.lastname: Stochmal
authors.forename: Wieslaw
authors.initials: W
authors.lastname: Oleszek
mesh_terms: D000328:Adult; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism; D001798:Blood Proteins / Q000737:chemistry; D006801:Humans; D008055:Lipids / Q000737:chemistry; D018384:Oxidative Stress / Q000187:drug effects*; D030421:Peroxynitrous Acid / Q000737:chemistry / Q000494:pharmacology*; D035261:Plant Components, Aerial / Q000737:chemistry*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D010949:Plasma / Q000187:drug effects*; D050050:Protein Carbonylation / Q000187:drug effects; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D029921:Trifolium; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D055815:Young Adult
chemical_list: D001798:Blood Proteins; C518724:CML-1 plant extract; D008055:Lipids; D010936:Plant Extracts; D017392:Thiobarbituric Acid Reactive Substances; D030421:Peroxynitrous Acid; C002744:3-nitrotyrosine; D014443:Tyrosine; C416518:clovamide