title: In vivo O2 measurement inside single photosynthetic cells.
abstract: The oxygen evolution of single cells was investigated using a nano-probe with an ultra-micro electrode (UME) in a submicron sized system in combination with a micro-fluidic system. A single cell was immobilized in the micro-fluidic system and a nano-probe was inserted into the cytosolic space of the single cell. Then, the UME was used for an in vivo amperometric experiment at a fixed potential and electrochemical impedance spectroscopy to detect oxygen evolution of the single cell under various light intensities.
authors.affiliation: Department of Mechanical Engineering, Dankook University, Yong-in, Republic of Korea. sbai@dankook.ac.kr
authors.forename: Seoung-Jai
authors.initials: SJ
authors.lastname: Bai
authors.forename: WonHyoung
authors.initials: W
authors.lastname: Ryu
authors.forename: Rainer J
authors.initials: RJ
authors.lastname: Fasching
authors.forename: Arthur R
authors.initials: AR
authors.lastname: Grossman
authors.forename: Fritz B
authors.initials: FB
authors.lastname: Prinz
mesh_terms: D016825:Chlamydomonas reinhardtii / Q000166:cytology / Q000502:physiology; D058266:Dielectric Spectroscopy / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D008839:Microelectrodes; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D010100:Oxygen / Q000032:analysis*; D010788:Photosynthesis; D059010:Single-Cell Analysis / Q000295:instrumentation* / Q000379:methods*
chemical_list: D010100:Oxygen