title: Identification of the peripheral blood levels of interleukin-12, interleukin-10, and transforming growth factor-β in patients with laryngeal squamous cell carcinoma.
abstract: BACKGROUND
  The aim of this study was to investigate the biology of laryngeal squamous cell carcinoma (SCC) to develop effective novel treatment modalities.
  
  
  METHODS
  Serum concentrations of interleukin (IL)-10, IL-12, and transforming growth factor-β (TGF-β) were evaluated in 50 patients with laryngeal SCC and 15 controls. Results were compared according to tumor-node-metastasis (TNM) classification criteria.
  
  
  RESULTS
  IL-12 and TGF-β levels were not different between the early- and late-stage patients and controls. Tumor classification or nodal involvement was not associated with IL-12 and TGF-β levels. Patients with laryngeal SCC had significantly more detectable serum IL-10 levels than those of controls, given that IL-10 could be detected in only 1 early-stage and 9 late-stage patients, but not in the control group (p = .003). IL-10 was increasingly detectable with advanced T classification (p = .009) and nodal involvement (p = .008).
  
  
  CONCLUSIONS
  Serum IL-12 or TGF-β levels were not affected with disease activity and classification; however, serum IL-10 levels were correlated with both parameters.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Hacettepe University, Ankara, Turkey.
authors.forename: Rıza Önder
authors.initials: RÖ
authors.lastname: Günaydın
authors.forename: Sacit Altuğ
authors.initials: SA
authors.lastname: Kesikli
authors.forename: Emin
authors.initials: E
authors.lastname: Kansu
authors.forename: Ali Şefik
authors.initials: AŞ
authors.lastname: Hoşal
mesh_terms: D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000097:blood* / Q000473:pathology; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D016753:Interleukin-10 / Q000097:blood*; D018664:Interleukin-12 / Q000097:blood*; D007822:Laryngeal Neoplasms / Q000097:blood* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D016212:Transforming Growth Factor beta / Q000097:blood*
chemical_list: D016212:Transforming Growth Factor beta; D016753:Interleukin-10; D018664:Interleukin-12