title: Potent induction of IFN-γ production from cord blood NK cells by the stimulation with single-stranded RNA.
abstract: Natural killer (NK) cells play important roles in the innate immunity against viral infections. Although newborn infants are more susceptible to severe and recurrent viral infections than adults, the precise role of NK cells in the innate immunity against viral infections during neonatal period is not known. To clarify the functional characteristics of cord blood (CB) NK cells, we examined the capacity of CB NK cells to produce interferon gamma (IFN-γ) in response to the Toll-like receptor (TLR) ligands. We found that NK cells produced a large amount of IFN-γ by the stimulation with ssRNA, a TLR8 ligand, in the presence of interleukin-2 (IL-2), Interferon alpha (INF-α), and monocytes. Surprisingly, CB NK cells produced higher amount of IFN-γ than adult peripheral blood NK cells in this condition. IL-12 produced from monocytes by the stimulation with ssRNA was indispensable for the production of IFN-γ by NK cells. NK cells in cooperation with other innate immune cells may play more important role during the neonatal period than in adults in the host defense against viral infections by high capacity of IFN-γ production to compensate immature acquired immunity.
authors.affiliation: Department of Pediatrics, Graduate School of Medical Sciences, Kyushu University, 3-1-1, Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.
authors.forename: Fairuz Mohamed
authors.initials: FM
authors.lastname: Eljaafari
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Takada
authors.forename: Tamami
authors.initials: T
authors.lastname: Tanaka
authors.forename: Takehiko
authors.initials: T
authors.lastname: Doi
authors.forename: Shouichi
authors.initials: S
authors.lastname: Ohga
authors.forename: Toshiro
authors.initials: T
authors.lastname: Hara
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000096:biosynthesis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000096:biosynthesis; D003470:Culture Media / Q000737:chemistry; D003602:Cytotoxicity, Immunologic; D005312:Fetal Blood / Q000166:cytology* / Q000276:immunology*; D006801:Humans; D016898:Interferon-alpha / Q000378:metabolism; D007371:Interferon-gamma / Q000096:biosynthesis*; D018664:Interleukin-12 / Q000096:biosynthesis / Q000378:metabolism; D007376:Interleukin-2 / Q000378:metabolism; D007694:Killer Cells, Natural / Q000276:immunology*; D037181:Lectins, C-Type / Q000096:biosynthesis; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D012313:RNA / Q000378:metabolism*; D051200:Toll-Like Receptor 8 / Q000037:antagonists & inhibitors; D000099192:CD69 Antigens
chemical_list: D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D003470:Culture Media; D016898:Interferon-alpha; D007371:Interferon-gamma; D018664:Interleukin-12; D007376:Interleukin-2; D037181:Lectins, C-Type; D012313:RNA; D051200:Toll-Like Receptor 8; D000099192:CD69 Antigens