title: [Expressions of cysteine-rich secretory protein 2 in asthenospermia].
abstract: OBJECTIVE
  To investigate the mRNA and protein expression levels of cysteine-rich secretory protein 2 (CRISP2) in the sperm of asthenospermia patients, and explore their relationship with sperm motility and related molecular mechanism.
  
  
  METHODS
  We collected 78 semen samples from adult male patients with asthenospermia and another 70 from healthy volunteers as controls. We extracted total RNA and total protein from the sperm following purification of the sperm by Percoll gradient centrifugation, and detected the relative expressions of CRISP2 mRNA and protein in the two groups by RT-PCR, SYBR Green real-time PCR and Western blot.
  
  
  RESULTS
  The expression of CRISP2 mRNA was down-regulated by 4.3 times and that of the CRISP2 protein by 1.71 times in the asthenospermia patients, significantly lower than in the normal control group (P < 0.05).
  
  
  CONCLUSIONS
  The down-regulation of CRISP2 mRNA and protein expressions in the sperm of asthenospermia patients may be closely related with decreased sperm motility, which suggests that CRISP2 may serve as a potential molecular target for the research of asthenospermia.
authors.affiliation: Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China. jing0326@gmail.com
authors.forename: Xiao-wei
authors.initials: XW
authors.lastname: Jing
authors.forename: Rong-wei
authors.initials: RW
authors.lastname: Xing
authors.forename: Qi-zhao
authors.initials: QZ
authors.lastname: Zhou
authors.forename: Qing-feng
authors.initials: QF
authors.lastname: Yu
authors.forename: Wen-bin
authors.initials: WB
authors.lastname: Guo
authors.forename: Si-mei
authors.initials: SM
authors.lastname: Chen
authors.forename: Qing-jun
authors.initials: QJ
authors.lastname: Chu
authors.forename: Chun-qiong
authors.initials: CQ
authors.lastname: Feng
authors.forename: Xiang-ming
authors.initials: XM
authors.lastname: Mao
mesh_terms: D000328:Adult; D053627:Asthenozoospermia / Q000235:genetics / Q000378:metabolism*; D016022:Case-Control Studies; D015815:Cell Adhesion Molecules; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008297:Male; D013081:Sperm Motility; D013094:Spermatozoa / Q000378:metabolism* / Q000502:physiology
chemical_list: C062621:CRISP2 protein, human; D015815:Cell Adhesion Molecules; D006023:Glycoproteins