title: No effects of pulsed electromagnetic fields on expression of cell adhesion molecules (integrin, CD44) and matrix metalloproteinase-2/9 in osteosarcoma cell lines.
abstract: Pulsed electromagnetic fields (PEMF) could enhance the cytocidal effects of chemotherapeutic drugs on malignant tumor cell lines, but metastasis effects of PEMF on tumor cells have not been investigated. We investigated the effects of PEMF exposure on the expression levels of some metastasis-related molecules, including integrin α subunits (α1, α2, α3, α4, α5, α6, αv), integrin β subunits (β1, β2, β3, β4), CD44, and matrix metalloproteinase-2/9 (MMP-2/9) in four human osteosarcoma cell lines (HOS, MG-63, SAOS-2, NY) and two mouse osteosarcoma cell lines (DOS, LM8) by using FACScan analysis, gelatin zymography, and Western blot analysis. Our results indicate that PEMF exposure has no effect on the expression of some molecules that are associated with tumor cell invasion and metastasis, and therefore suggest that PEMF exposure may be safely applied to chemotherapy for osteosarcoma.
authors.affiliation: Department of Orthopaedic Surgery, Aichi Medical University School of Medicine, Nagakute, Aich, Japan.
authors.forename: Daguang
authors.initials: D
authors.lastname: Zhang
authors.forename: Xin
authors.initials: X
authors.lastname: Pan
authors.forename: Shuichiro
authors.initials: S
authors.lastname: Ohno
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Osuga
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Sawada
authors.forename: Keiji
authors.initials: K
authors.lastname: Sato
mesh_terms: D000818:Animals; D001859:Bone Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000628:therapy; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D045744:Cell Line, Tumor; D004574:Electromagnetic Fields; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism; D039001:Integrin alpha Chains / Q000378:metabolism; D039641:Integrin beta Chains / Q000378:metabolism; D055909:Magnetic Field Therapy; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D051379:Mice; D012516:Osteosarcoma / Q000378:metabolism* / Q000473:pathology / Q000628:therapy
chemical_list: C497483:CD44 protein, human; D015815:Cell Adhesion Molecules; D018960:Hyaluronan Receptors; D039001:Integrin alpha Chains; D039641:Integrin beta Chains; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9