title: [Effects of Chinese herbal medicine Jinqi Jiangtang Tablet on serum matrix metalloproteinase-9 level and its expression in peripheral blood monocytes of rats with diabetes mellitus].
abstract: OBJECTIVE
  To observe the effects of Jinqi Jiangtang Tablet, a compound Chinese herbal medicine, on serum matrix metalloproteinase-9 (MMP-9) level and MMP-9 expression in peripheral blood monocytes of rats with streptozocin-induced diabetes.
  
  
  METHODS
  Thirty Wistar rats were randomly divided into 3 groups: control group, untreated group and Jinqi Jiangtang Tablet group. Diabetes in rats was induced by a single intraperitoneal injection of streptozocin (70mg/kg body weight). After model building, rats in the Jinqi Jiangtang Tablet group were administered with Jinqi Jiangtang tablets at a dose of 0.8 g/(kg·d) for 6 weeks and rats in the control group and the untreated group were treated with normal saline. After treatment, level of fasting serum glucose was detected by hexokinas method; serum MMP-9 level was tested by enzyme-linked immunosorbent assay; MMP-9 expression in peripheral blood monocytes was measured by reverse transcription-polymerase chain reaction and Western blot method.
  
  
  RESULTS
  After treatment, fasting serum glucose level in the Jinqi Jiangtang Tablet group was less than that in the untreated group (P<0.05). After modeling, the serum MMP-9 level of rats in the untreated group was (9.11±3.55) μg/L and that in Jinqi Jiangtang Tablet group was (8.97± 3.07) μg/L, which were higher than that in the control group ((4.39±1.44) μg/L) (P<0.01). After gavage, the level of serum MMP-9 was lower in Jinqi Jiangtang Tablet group than in the untreated group (P<0.05). The expression of MMP-9 in peripheral blood monocytes in the untreated group was higher than that in the control group (P<0.05). The MMP-9 expression in peripheral blood monocytes of rats with diabetes was down-regulated by Jinqi Jiangtang Tablet (P<0.05).
  
  
  CONCLUSIONS
  Jinqi Jiangtang Tablet can decrease the level of serum MMP-9 and down-regulate the MMP-9 expression in peripheral blood monocytes at both mRNA and protein levels in rats with diabetes.
authors.affiliation: Department of Geriatric Cardiology, Chinese PLA General Hospital, Beijing 100853, China. wanghao2003@sina.com
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.forename: Lin
authors.initials: L
authors.lastname: Shen
authors.forename: Zhi-guo
authors.initials: ZG
authors.lastname: Wang
authors.forename: Yi
authors.initials: Y
authors.lastname: Cao
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000097:blood* / Q000188:drug therapy; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D005260:Female; D007004:Hypoglycemic Agents / Q000627:therapeutic use*; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000097:blood*; D009000:Monocytes / Q000378:metabolism; D008517:Phytotherapy; D051381:Rats; D017208:Rats, Wistar; D044967:Serum / Q000378:metabolism*
chemical_list: D004365:Drugs, Chinese Herbal; D007004:Hypoglycemic Agents; C529953:Jinqi; D020780:Matrix Metalloproteinase 9