title: Combination of monoclonal antibodies with DST inhibits accelerated rejection mediated by memory T cells to induce long-lived heart allograft acceptance in mice.
abstract: Donor-reactive memory T cells mediated accelerated rejection is known as a barrier to the survival of transplanted organs. We investigated the combination of different monoclonal antibodies (mAbs) and donor-specific transfusion (DST) in memory T cells-based adoptive mice model. In the presence of donor-reactive memory T cells, the mean survival time (MST) of grafts in the anti-CD40L/LFA-1/DST group was 49.8d. Adding anti-CD44/CD70 mAbs to anti-CD40L/LFA-1/DST treatment. The MST was more than 100 d (MST>100 d). Compared with anti-CD40L/LFA-1/DST group, anti-CD40L/LFA-1/CD44/CD70/DST group notably reduced the expansion of memory T cells, enhanced the proportion of CD4+Foxp3+ regulatory T cells (Tregs) and suppressed donor-specific responses. Our data suggest that anti-CD40L/LFA-1/CD44/CD70mAbs and DST can synergistically inhibit accelerated rejection mediated by memory T cells to induce long-lived heart allograft acceptance in mice.
authors.affiliation: School of Life Sciences, Xiamen University, Fujian Province, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Shao
authors.forename: Jibing
authors.initials: J
authors.lastname: Chen
authors.forename: Helong
authors.initials: H
authors.lastname: Dai
authors.forename: Yuanzheng
authors.initials: Y
authors.lastname: Peng
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: Junjie
authors.initials: J
authors.lastname: Xia
authors.forename: Henrik
authors.initials: H
authors.lastname: Thorlacius
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhu
authors.forename: Zhongquan
authors.initials: Z
authors.lastname: Qi
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology; D053282:CD27 Ligand / Q000037:antagonists & inhibitors / Q000276:immunology; D023201:CD40 Ligand / Q000037:antagonists & inhibitors / Q000276:immunology; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D006084:Graft Rejection / Q000276:immunology / Q000517:prevention & control; D006085:Graft Survival / Q000276:immunology*; D016027:Heart Transplantation / Q000276:immunology; D018960:Hyaluronan Receptors / Q000276:immunology; D007156:Immunologic Memory; D056747:Immunomodulation; D007519:Isoantigens / Q000276:immunology*; D016169:Lymphocyte Function-Associated Antigen-1 / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009000:Monocytes / Q000276:immunology / Q000637:transplantation; D016038:Skin Transplantation / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D014184:Transplantation, Homologous
chemical_list: D000911:Antibodies, Monoclonal; D053282:CD27 Ligand; C476399:Cd44 protein, mouse; D018960:Hyaluronan Receptors; D007519:Isoantigens; D016169:Lymphocyte Function-Associated Antigen-1; D023201:CD40 Ligand