title: Antimicrobial susceptibility and PFGE genotyping of Haemophilus parasuis isolates from pigs in South China (2008-2010).
abstract: H. parasuis isolates (n=112) from pigs were tested for antimicrobial susceptibility against 23 antimicrobial agents by the disk diffusion method. All isolates were sensitive to Florfenico and most strains were sensitive to Cefotaxime (103/112; 92%), Ceftazidime (99/112; 88.4%), Chloramphenicol (90/112; 80.4%) and Gentamicin (85/112; 75.9%). High resistance levels to Nalidixic acid (84.8%), TMP (67.9%) and Trimethoprim+Sulfamethoxazole (58%) were observed. Genomic DNA extracted from 52 isolates resistant to at least seven antimicrobial agents was analyzed by PFGE and 46 distinct PFGE patterns identified. Diverse variation was observed between the drug-resistant H. parasuis isolates examined, suggesting that resistance traits were acquired independently by the respective isolates.
authors.affiliation: Key Laboratory of Animal Disease Control and Prevention of the Ministry of Agriculture, Key Laboratory of Zoonoses Prevention and Control of Guangdong, College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province, China.
authors.forename: Chenggang
authors.initials: C
authors.lastname: Xu
authors.forename: Jianmin
authors.initials: J
authors.lastname: Zhang
authors.forename: Zhanqin
authors.initials: Z
authors.lastname: Zhao
authors.forename: Lili
authors.initials: L
authors.lastname: Guo
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.forename: Saixiang
authors.initials: S
authors.lastname: Feng
authors.forename: Lingyun
authors.initials: L
authors.lastname: Zhang
authors.forename: Ming
authors.initials: M
authors.lastname: Liao
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002681:China; D052978:Disk Diffusion Antimicrobial Tests / Q000662:veterinary; D024881:Drug Resistance, Bacterial; D024901:Drug Resistance, Multiple, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field / Q000662:veterinary; D005838:Genotype; D060005:Genotyping Techniques / Q000662:veterinary; D006192:Haemophilus Infections / Q000382:microbiology / Q000662:veterinary*; D044137:Haemophilus parasuis / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*
chemical_list: D000900:Anti-Bacterial Agents