title: RNA interference of WdFKS1 mRNA expression causes slowed growth, incomplete septation and loss of cell wall integrity in yeast cells of the polymorphic, pathogenic fungus Wangiella (Exophiala) dermatitidis.
abstract: As one of the main components of the fungal cell wall, β-1,3-glucan provides the mechanical strength to protect fungal protoplasts. The enzyme responsible for the synthesis of β-1,3-glucans in fungi is β-1,3-glucan synthase. Here we report the cloning, sequencing and characterization of the WdFKS1 gene, which in the pathogenic fungus Wangiella dermatitidis encodes the catalytic domain of its β-1, 3-glucan synthase. Because our research suggested that WdFKS1 is a single copy essential gene, we used RNA interference to reduce its expression. Reduction of the WdFKS1 messenger retarded growth and caused the loss of cell wall integrity of yeast cells, but not hyphae or sclerotic cells. We suggest that the WdFKS1 in this polymorphic agent of phaeohyphomycosis is not only required for cell wall construction and maintenance, but also is involved in septum formation.
authors.affiliation: The Section of Molecular Genetics and Microbiology, The University of Texas at Austin, Austin, Texas 78712, USA.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Guo
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Szaniszlo
mesh_terms: D001483:Base Sequence; D020134:Catalytic Domain / Q000235:genetics; D002473:Cell Wall / Q000201:enzymology / Q000502:physiology; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D005093:Exophiala / Q000201:enzymology / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D015698:Genomic Library; D005964:Glucosyltransferases / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D025301:Hyphae / Q000201:enzymology / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D008969:Molecular Sequence Data; D060446:Phaeohyphomycosis / Q000382:microbiology*; D034622:RNA Interference / Q000502:physiology*; D012331:RNA, Fungal / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D017422:Sequence Analysis, DNA; D047071:beta-Glucans / Q000378:metabolism*
chemical_list: D018076:DNA, Complementary; D004271:DNA, Fungal; D005656:Fungal Proteins; D012331:RNA, Fungal; D012333:RNA, Messenger; D047071:beta-Glucans; C033363:beta-1,3-glucan; D005964:Glucosyltransferases; C040232:glucan synthase