title: Cells producing insulin-like androgenic gland hormone of the giant freshwater prawn, Macrobrachium rosenbergii, proliferate following bilateral eyestalk-ablation.
abstract: We found that the androgenic gland (AG) of Macrobrachium rosenbergii possesses three cell types. Type I cells are small polygonal shaped-cells (13.4 μm in diameter), stain strongly with hematoxylin-eosin (H&E), have abundant multilayered rough endoplasmic reticulum (rER), and nuclei containing mostly heterochromatin. Type II cells are slightly larger (18.6 μm in diameter), stain lightly with H&E, have rER with dilated cisternae, and nuclei containing mostly euchromatin. Type III cells (previously undescribed) are similar in size and shape to type I cells, but the cytoplasm is unstained and they have a high amount of smooth endoplasmic reticulum (sER) and mitochondria with tubular cristae. Bilateral eyestalk-ablation resulted in AG hypertrophy with a proliferation and predominance of type I cells as determined by bromodeoxyuridine (BrdU) assays. Expression of insulin-like androgenic gland hormone (Mr-IAG), determined by immunohistochemistry, was weak in type I cells, strong in type II cells of both the intact and eyestalk-ablated, and negative in type III cells. It was also detected in spermatogonia, nurse cells, and epithelium lining of the spermatic duct. The function of Mr-IAG in these tissues is yet to be elucidated but the distribution implies a strong role in male reproduction.
authors.affiliation: Department of Anatomy, Faculty of Science, Mahidol University, Rama VI Road, Ratchathewi, Bangkok 10400, Thailand. ittiponnu47@yahoo.com
authors.forename: Ittipon
authors.initials: I
authors.lastname: Phoungpetchara
authors.forename: Yotsawan
authors.initials: Y
authors.lastname: Tinikul
authors.forename: Jaruwan
authors.initials: J
authors.lastname: Poljaroen
authors.forename: Charoonroj
authors.initials: C
authors.lastname: Chotwiwatthanakun
authors.forename: Rapeepun
authors.initials: R
authors.lastname: Vanichviriyakit
authors.forename: Morakot
authors.initials: M
authors.lastname: Sroyraya
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Hanna
authors.forename: Prasert
authors.initials: P
authors.lastname: Sobhon
mesh_terms: D000728:Androgens / Q000378:metabolism; D000825:Animal Structures / Q000502:physiology*; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D049109:Cell Proliferation; D018870:Endoplasmic Reticulum, Rough / Q000648:ultrastructure; D005088:Exocrine Glands / Q000166:cytology* / Q000648:ultrastructure; D005123:Eye; D007447:Invertebrate Hormones / Q000378:metabolism*; D008297:Male; D008928:Mitochondria / Q000378:metabolism; D033541:Palaemonidae / Q000378:metabolism*; D013002:Somatomedins / Q000096:biosynthesis*
chemical_list: D000728:Androgens; D007447:Invertebrate Hormones; D013002:Somatomedins