title: Osteoblastlike cell adhesion on titanium surfaces modified by plasma nitriding.
abstract: OBJECTIVE
  The aim of this study was to evaluate the characteristics of various titanium surfaces modified by cold plasma nitriding in terms of adhesion and proliferation of rat osteoblastlike cells.
  
  
  METHODS
  Samples of grade 2 titanium were subjected to three different surface modification processes: polishing, nitriding by plasma direct current, and nitriding by cathodic cage discharge. To evaluate the effect of the surface treatment on the cellular response, the adhesion and proliferation of osteoblastlike cells (MC3T3) were quantified and the results were analyzed by Kruskal-Wallis and Friedman statistical tests. Cellular morphology was observed by scanning electron microscopy.
  
  
  RESULTS
  There was more MC3T3 cell attachment on the rougher surfaces produced by cathodic cage discharge compared with polished samples (P < .05).
  
  
  CONCLUSIONS
  Plasma nitriding improves titanium surface roughness and wettability, leading to osteoblastlike cell adhesion.
authors.affiliation: Departamento de Odontologia, Universidade Federal do Rio Grande do Norte, Natal/RN, Brazil. jspsilva@gmail.com
authors.forename: Jose Sandro Pereira
authors.initials: JS
authors.lastname: da Silva
authors.forename: Sandro Campos
authors.initials: SC
authors.lastname: Amico
authors.forename: Almir Olegario Neves
authors.initials: AO
authors.lastname: Rodrigues
authors.forename: Carlos Augusto Galvao
authors.initials: CA
authors.lastname: Barboza
authors.forename: Clodomiro
authors.initials: C
authors.lastname: Alves
authors.forename: Alberto Tesconi
authors.initials: AT
authors.lastname: Croci
mesh_terms: D000818:Animals; D017554:Carbon Compounds, Inorganic / Q000737:chemistry; D002448:Cell Adhesion / Q000502:physiology; D002452:Cell Count; D002460:Cell Line; D049109:Cell Proliferation; D048430:Cell Shape; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D003764:Dental Materials / Q000737:chemistry*; D003775:Dental Polishing / Q000379:methods; D004595:Electroplating / Q000379:methods; D006861:Hydrogen Peroxide / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D017672:Nitrogen Compounds / Q000737:chemistry*; D010006:Osteoblasts / Q000502:physiology* / Q000648:ultrastructure; D016877:Oxidants / Q000737:chemistry; D058626:Plasma Gases / Q000737:chemistry*; D051381:Rats; D017655:Silicon Compounds / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*; D017155:Wettability
chemical_list: D017554:Carbon Compounds, Inorganic; D020099:Coated Materials, Biocompatible; D003764:Dental Materials; D017672:Nitrogen Compounds; D016877:Oxidants; D058626:Plasma Gases; D017655:Silicon Compounds; D012822:Silicon Dioxide; D006861:Hydrogen Peroxide; D014025:Titanium; C022088:silicon carbide