title: Interleukin-10 gene polymorphism reflects the severity of chronic immune thrombocytopenia in Japanese patients.
abstract: BACKGROUND
  T-helper cell type 1 (Th1) polarization of the immune response has been documented in patients with chronic immune thrombocytopenia (ITP). Interleukin (IL)-10 is the most important factor regulating Th1 and T-helper type 2 cytokine synthesis. This study evaluated the impact of IL-10 polymorphisms on both susceptibility to, and severity of, chronic ITP.
  
  
  METHODS
  We analyzed -1082(G/A), -812(C/T), and -592(C/A) IL-10 polymorphisms in 90 patients with adult chronic ITP and 202 race- and sex-matched healthy controls.
  
  
  RESULTS
  No significant differences in the genotype or haplotype frequencies were observed between the patient with chronic ITP and the control group. However, more patients with the -592AA genotype showed a severe thrombocytopenic state (platelet count <10 x 10⁹/l) than those with the -592CC/CA genotypes (44.1%vs. 19.6%, P = 0.01). Furthermore, more patients with the ATA/ATA haplotype showed a severe thrombocytopenic state than those without the ATA/ATA haplotype (44.1%vs. 19.6%, P = 0.01).
  
  
  CONCLUSIONS
  According to our data, patients with low producer type of IL-10 polymorphisms have more severe thrombocytopenia, suggesting that IL-10 gene polymorphisms may reflect the severity of ITP.
authors.affiliation: Department of Medicine and Clinical Science, Gunma University Graduate School of Medicine, Gunma, Japan. tsaitoh@showa.gunma-u.ac.jp
authors.forename: T
authors.initials: T
authors.lastname: Saitoh
authors.forename: T
authors.initials: T
authors.lastname: Kasamatsu
authors.forename: M
authors.initials: M
authors.lastname: Inoue
authors.forename: T
authors.initials: T
authors.lastname: Mitsui
authors.forename: H
authors.initials: H
authors.lastname: Koiso
authors.forename: A
authors.initials: A
authors.lastname: Yokohama
authors.forename: H
authors.initials: H
authors.lastname: Handa
authors.forename: T
authors.initials: T
authors.lastname: Matsushima
authors.forename: N
authors.initials: N
authors.lastname: Tsukamoto
authors.forename: M
authors.initials: M
authors.lastname: Karasawa
authors.forename: H
authors.initials: H
authors.lastname: Ogawara
authors.forename: Y
authors.initials: Y
authors.lastname: Nojima
authors.forename: H
authors.initials: H
authors.lastname: Murakami
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D044466:Asian People / Q000235:genetics; D002908:Chronic Disease; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D007564:Japan; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011401:Promoter Regions, Genetic; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000235:genetics* / Q000628:therapy; D016896:Treatment Outcome; D055815:Young Adult
chemical_list: D016753:Interleukin-10