title: [Effect of tissue factor in extravascular migration of fibrosarcoma cells].
abstract: OBJECTIVE
  To evaluate the effect of tissue factor (TF) in extravascular migration of fibrosarcoma cells and hematogenous metastasis.
  
  
  METHODS
  The expression of tissue factor in fibrosarcoma HT1080 cells was analyzed by flow cytometry. The extravascular migration of fibrosarcoma cells was observed in a constructed monolayer vascular endothelial cells and extra-cellular matrix model.
  
  
  RESULTS
  Tissue factor was highly expressed in HT1080 cells. HT1080 migrated and passed through the monolayer vascular endothelial cells to the collagen gel in a time-dependent manner. Anti-TF antibody inhibited extravascular migration of fibrosarcoma cells and the inhibition was concentration-dependent (P<0.05).
  
  
  CONCLUSIONS
  Tissue factor may enhance hematogenous metastasis through extravascular migration of fibrosarcoma cells.
authors.affiliation: Department of Thoracic Surgery, China-Japan Union Hospital, Jilin University, Changchun 130033, China.
authors.forename: Chun-Shan
authors.initials: CS
authors.lastname: Han
authors.forename: Hua
authors.initials: H
authors.lastname: Xin
authors.forename: Zhen-Guo
authors.initials: ZG
authors.lastname: Han
authors.forename: Zhong-Li
authors.initials: ZL
authors.lastname: Gao
mesh_terms: D002465:Cell Movement; D002478:Cells, Cultured; D042783:Endothelial Cells; D005354:Fibrosarcoma / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D009362:Neoplasm Metastasis; D013925:Thromboplastin / Q000378:metabolism / Q000502:physiology*
chemical_list: D013925:Thromboplastin