title: Pharmacological characterization of chemically synthesized monomeric phi29 pRNA nanoparticles for systemic delivery.
abstract: Previous studies have shown that the packaging RNA (pRNA) of bacteriophage phi29 DNA packaging motor folds into a compact structure, constituting a RNA nanoparticle that can be modularized with functional groups as a nanodelivery system. pRNA nanoparticles can also be self-assembled by the bipartite approach without altering folding property. The present study demonstrated that 2'-F-modified pRNA nanoparticles were readily manufactured through this scalable bipartite strategy, featuring total chemical synthesis and permitting diverse functional modularizations. The RNA nanoparticles were chemically and metabolically stable and demonstrated a favorable pharmacokinetic (PK) profile in mice (half-life (T(1/2)): 5-10 hours, clearance (Cl): <0.13 l/kg/hour, volume of distribution (V(d)): 1.2 l/kg). It did not induce an interferon (IFN) response nor did it induce cytokine production in mice. Repeat intravenous administrations in mice up to 30 mg/kg did not result in any toxicity. Fluorescent folate-pRNA nanoparticles efficiently and specifically bound and internalized to folate receptor (FR)-bearing cancer cells in vitro. It also specifically and dose-dependently targeted to FR(+) xenograft tumor in mice with minimal accumulation in normal tissues. This first comprehensive pharmacological study suggests that the pRNA nanoparticle had all the preferred pharmacological features to serve as an efficient nanodelivery platform for broad medical applications.
authors.affiliation: Kylin Therapeutics, Inc, West Lafayette, Indiana 47906, USA.
authors.forename: Sherine
authors.initials: S
authors.lastname: Abdelmawla
authors.forename: Songchuan
authors.initials: S
authors.lastname: Guo
authors.forename: Limin
authors.initials: L
authors.lastname: Zhang
authors.forename: Sai M
authors.initials: SM
authors.lastname: Pulukuri
authors.forename: Prithviraj
authors.initials: P
authors.lastname: Patankar
authors.forename: Patrick
authors.initials: P
authors.lastname: Conley
authors.forename: Joseph
authors.initials: J
authors.lastname: Trebley
authors.forename: Peixuan
authors.initials: P
authors.lastname: Guo
authors.forename: Qi-Xiang
authors.initials: QX
authors.lastname: Li
mesh_terms: D000818:Animals; D001435:Bacteriophages / Q000235:genetics; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D018613:Microscopy, Confocal; D053758:Nanoparticles / Q000737:chemistry*; D012313:RNA / Q000008:administration & dosage / Q000138:chemical synthesis* / Q000737:chemistry / Q000235:genetics*; D034741:RNA, Small Interfering / Q000008:administration & dosage*; D012367:RNA, Viral / Q000737:chemistry* / Q000235:genetics
chemical_list: D034741:RNA, Small Interfering; D012367:RNA, Viral; D012313:RNA