title: Evisceration of mouse vitreous and retina for proteomic analyses.
abstract: While the mouse retina has emerged as an important genetic model for inherited retinal disease, the mouse vitreous remains to be explored. The vitreous is a highly aqueous extracellular matrix overlying the retina where intraocular as well as extraocular proteins accumulate during disease.(1-3) Abnormal interactions between vitreous and retina underlie several diseases such as retinal detachment, proliferative diabetic retinopathy, uveitis, and proliferative vitreoretinopathy.(1,4) The relative mouse vitreous volume is significantly smaller than the human vitreous (Figure 1), since the mouse lens occupies nearly 75% of its eye.(5) This has made biochemical studies of mouse vitreous challenging. In this video article, we present a technique to dissect and isolate the mouse vitreous from the retina, which will allow use of transgenic mouse models to more clearly define the role of this extracellular matrix in the development of vitreoretinal diseases.
authors.affiliation: Omics Laboratory, University of Iowa.
authors.forename: Jessica M
authors.initials: JM
authors.lastname: Skeie
authors.forename: Stephen H
authors.initials: SH
authors.lastname: Tsang
authors.forename: Vinit B
authors.initials: VB
authors.lastname: Mahajan
mesh_terms: D000818:Animals; D017234:Eye Evisceration / Q000379:methods*; D005136:Eye Proteins / Q000032:analysis*; D051379:Mice; D023421:Models, Animal; D040901:Proteomics / Q000379:methods*; D012160:Retina / Q000737:chemistry* / Q000601:surgery*; D014822:Vitreous Body / Q000737:chemistry* / Q000601:surgery*
chemical_list: D005136:Eye Proteins