title: Expression and function of P2X(7) receptor and CD39/Entpd1 in patients with type 2 diabetes and their association with biochemical parameters.
abstract: Chronic inflammation is an important contributor to the insulin resistance observed in type 2 diabetes (T2D). We evaluated the expression and function of the P2X(7) receptor and CD39/Entpd1, molecules involved in the cellular regulation of inflammation, in peripheral blood mononuclear cells from T2D patients, and their correlation with the concentration of HbA1c in blood. T2D patients with deficient metabolic control (DC) showed increased proportion of P2X(7)(+) cells compared with healthy individuals; T2D-DC subjects also displayed higher proportion of CD14(+), CD4(+) and CD19(+) subpopulations of P2X(7)(+) cells when compared with T2D patients with acceptable metabolic control. A significant association was observed between the proportion of P2X(7)(+)CD14(+) cells and blood concentration of LDL-c. In addition, the percentages of CD39(+) cells and CD39(+)CD19(+) cells were significantly associated with HbA1c and fasting plasma glucose levels. No changes were observed in the function of P2X(7)(+) cells from T2D patients; however, enhanced CD39/Entpd1 enzyme activity and low serum levels of IL-17 were detected. Therefore, CD39(+) cells could have a balancing regulatory role in the inflammatory process observed in patients with T2D.
authors.affiliation: Laboratorio de Inmunología y Biología Celular y Molecular, Facultad de Ciencias Químicas, UASLP, San Luis Potosí, SLP, Mexico.
authors.forename: Mariana H
authors.initials: MH
authors.lastname: García-Hernández
authors.forename: Liliana
authors.initials: L
authors.lastname: Portales-Cervantes
authors.forename: Nancy
authors.initials: N
authors.lastname: Cortez-Espinosa
authors.forename: Juan M
authors.initials: JM
authors.lastname: Vargas-Morales
authors.forename: Juan F
authors.initials: JF
authors.lastname: Fritche Salazar
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Rivera-López
authors.forename: Javier G
authors.initials: JG
authors.lastname: Rodríguez-Rivera
authors.forename: Roberto
authors.initials: R
authors.lastname: Quezada-Calvillo
authors.forename: Diana P
authors.initials: DP
authors.lastname: Portales-Pérez
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D000328:Adult; D015703:Antigens, CD / Q000378:metabolism*; D001081:Apyrase / Q000378:metabolism*; D001786:Blood Glucose / Q000378:metabolism; D008078:Cholesterol, LDL / Q000097:blood; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D005260:Female; D006442:Glycated Hemoglobin / Q000378:metabolism; D006801:Humans; D020381:Interleukin-17 / Q000097:blood; D053583:Interleukin-1beta / Q000378:metabolism; D019041:L-Selectin / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D008875:Middle Aged; D058848:Receptors, Purinergic P2X4 / Q000378:metabolism; D058486:Receptors, Purinergic P2X7 / Q000378:metabolism*; D055815:Young Adult
chemical_list: D000255:Adenosine Triphosphate; D015703:Antigens, CD; D001081:Apyrase; D001786:Blood Glucose; D008078:Cholesterol, LDL; D006442:Glycated Hemoglobin; D020381:Interleukin-17; D053583:Interleukin-1beta; D019041:L-Selectin; D008070:Lipopolysaccharides; D058848:Receptors, Purinergic P2X4; D058486:Receptors, Purinergic P2X7; C517652:hemoglobin A1c protein, human; C067869:CD39 antigen