title: Real-time cell-impedance sensing assay as an alternative to clonogenic assay in evaluating cancer radiotherapy.
abstract: Intrinsic radiosensitivity of normal and tumour tissues has been shown to be an independent prognostic factor for patients' response to radiotherapy. This study compares the real-time cell-impedance sensing (RT-CES) assay with the conventional clonogenic assay in terms of in-vitro radiosensitivity. One objective in this study was to predict in-vivo response to gold nanoparticle (GNP) treatment on the basis of in-vitro RT-CES testing results. Four adenocarcinoma cancer cell lines were tested using both the RT-CES and the clonogenic assays. Cell-survival curves were plotted, and the mean SF2 values obtained by these two different assay methods were compared using ANOVA. Radiation sensitivities obtained in-vitro were then compared with the in-vivo results. On the basis of the measurement of cell colonies, the RT-CES assay has similar radiosensitivity to the clonogenic assay, but significantly shortens the testing time from 14-21 days to only 72 h. Intrinsic GNP enhanced radiation sensitivity using tumour volume (mm(3)) in vivo is comparable with that using RT-CES cell survival assay in vitro. Furthermore, the RT-CES system provides real-time information regarding the state of cell radiosensitivity that may give useful information towards personalizing radiotherapy. The RT-CES assay enables more reliable and time-efficient results in the evaluation of radiosensitivity.
authors.affiliation: Division of Radiation Oncology, Cross Cancer Institute, Edmonton, Alberta, T6G 2V4, Canada.
authors.forename: Wilson
authors.initials: W
authors.lastname: Roa
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Yang
authors.forename: Linghong
authors.initials: L
authors.lastname: Guo
authors.forename: Biao
authors.initials: B
authors.lastname: Huang
authors.forename: Shima
authors.initials: S
authors.lastname: Khatibisepehr
authors.forename: Stephan
authors.initials: S
authors.lastname: Gabos
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.forename: James
authors.initials: J
authors.lastname: Xing
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D009369:Neoplasms / Q000473:pathology / Q000532:radiotherapy*; D011836:Radiation Tolerance; D016896:Treatment Outcome