title: [Mono-specific serum preparation and specificity of gp85 gene of subgroup A avian leukosis virus].
abstract: OBJECTIVE
  In order to get a rapid specific diagnostic reagent for subgroup A Avian Leukosis Virus detection.
  
  
  METHODS
  The Avian Leukosis Virus Subgroup A (ALV-A) SDAU09E1 strain was inoculated into DF1 cells, an ALV-A- gp85 DNA fragment of 1023 bp was amplified from infected cells and inserted into PET-32a(+) plasmid at the location between restriction endonucleases BamH I and Not I sites. The recombinant plasmid PET-SDAU09E1 -gp85 was transformed into E coli. BL21 (Rosetta) for gp85 gene expression. Then we used the purified recombinant fusion protein to immunize 6 weeks old Kunming white mice, and the antiserum were prepared.
  
  
  RESULTS
  The recombinant ALV-A gp85 fusion protein with a molecular weight of 52.8 kDa demonstrated a good antigenecity. Mon-specific serum produced by vaccinated mice came out reactive with subgroups A and B ALV (ALV-A and ALV-B but not subgroup J ALV) by the indirect immunofluorescence (IFA) method.
  
  
  CONCLUSIONS
  This was the first time to demonstrate a mono-specific antiserum specific to ALV-A and ALV-B, it could be used for differential diagnosis of exogenous ALV infections in CEF cultures when in complement with ALV-J specific monoclonal antibodies. Chickens in our country are now distressed by both classic ALV-A/B and emerging ALV-J, making differential diagnosis necessary, so studying this reagent has high practical value.
authors.affiliation: Animal Science and Technology College, Shandong Agricultural University, Tai'an 271018, China. zhangheng1987710@163.com
authors.forename: Heng
authors.initials: H
authors.lastname: Zhang
authors.forename: Chuanlong
authors.initials: C
authors.lastname: Li
authors.forename: Ming
authors.initials: M
authors.lastname: Yang
authors.forename: Zhizhong
authors.initials: Z
authors.lastname: Cui
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D000918:Antibody Specificity; D001353:Avian Leukosis / Q000175:diagnosis / Q000276:immunology* / Q000821:virology; D001354:Avian Leukosis Virus / Q000145:classification / Q000235:genetics / Q000276:immunology*; D002645:Chickens; D051379:Mice; D011201:Poultry Diseases / Q000175:diagnosis / Q000276:immunology* / Q000821:virology; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D014759:Viral Envelope Proteins