title: [Construction of a shuttle plasmid encoding a chimeric gene of eglA p-Her2/neu ECD-IL-12].
abstract: OBJECTIVE
  To construct a shuttle plasmid encoding a chimeric gene of Clostridium saccharobutylicum eglA promoter(eglA p)-extracellular domain of human epidermal growth factor receptors 2(hHer2/neu ECD)-human Interleukin-12(rhIL-12), pIMP1 eglA p-hHer 2/neu ECD-rhIL-12.
  
  
  METHODS
  The hHer2/neu ECD and the eglA p was amplified from the corresponding template: pcDNA3.1 hHer2/neu and 55 bp fragment in eglA p by PCR. pcDNA6 eglA p-hHer2/neu ECD-rhIL-12 was prepared by inserting the hHer2/neu ECD and eglAp fragment into the plasmid, pcDNA6 rhIL-12. Shuttle plasmid pIMP1 eglA p-hHer2/neu ECD-rhIL-12 was acquired by using in-fusion technique. subsequently, the recombinant shuttle plasmid was identified.
  
  
  RESULTS
  All the fragments of hHer2/neu ECD , eglA p and eglAp-Her2/neu ECD-rhIL-12 were amplified, and the corresponding plasmids were prepared rightly. The shuttle plasmid of pIMP1 eglAp-hHer2/neu ECD-rhIL-12 was successfully const- ructed. No error was found both in the sequence and ORF of the acquired chimeric gene.
  
  
  CONCLUSIONS
  A shuttle plasmid encoding a chimeric gene of Clostridium saccharobut- ylicum eglAp-hHer2/neu ECD-rhIL-12(pIMP1 eglA p-hHer2/neu ECD-rhIL- 12) was success- fully constructed.
authors.affiliation: Laboratory of Pathogenic Biology; Medical College, Qingdao University, Qingdao 266071, China. shenghua005@163.com
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Wen-qing
authors.initials: WQ
authors.lastname: Zhang
authors.forename: Hong
authors.initials: H
authors.lastname: Yu
authors.forename: Rui
authors.initials: R
authors.lastname: Lv
authors.forename: Yan-li
authors.initials: YL
authors.lastname: Zhang
authors.forename: Teng-fei
authors.initials: TF
authors.lastname: Xu
authors.forename: Dan
authors.initials: D
authors.lastname: Li
mesh_terms: D002480:Cellulase / Q000235:genetics*; D003013:Clostridium / Q000235:genetics*; D015316:Genetic Therapy; D018664:Interleukin-12 / Q000235:genetics*; D009369:Neoplasms / Q000628:therapy; D010957:Plasmids; D011401:Promoter Regions, Genetic; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics
chemical_list: D002480:Cellulase; D018664:Interleukin-12; D018719:Erb-b2 Receptor Tyrosine Kinases; D011993:Recombinant Fusion Proteins; C508053:ERBB2 protein, human