title: Lipoprotein enrichment in orange insoluble particulate matter reproducibly appearing in cryoprecipitate.
abstract: OBJECTIVE
  Cryoprecipitate prepared from two whole blood donations from the same donor contained insoluble orange particulate material (OPM). We sought to identify the OPM.
  
  
  METHODS
  OPM was recovered from the blood product by centrifugation, dissolved in sodium dodecyl sulphate (SDS) and analysed by SDS-polyacrylamide gel electrophoresis and immunoblotting.
  
  
  RESULTS
  Solubilized OPM was enriched in apolipoproteins B and E, but not apolipoprotein A1, immunoglobulin G or albumin, suggesting lipoprotein enrichment in OPM. Subsequent clinical laboratory blood tests confirmed low-density lipoprotein hyperlipidaemia with normal triglyceride levels. Further, cryoprecipitate production from this donor was prevented by implementation of national predominantly male plasma policies.
  
  
  CONCLUSIONS
  Cryoprecipitate produced from hyperlipidaemic donors may contain insoluble particles that render it inappropriate for transfusion.
authors.affiliation: Canadian Blood Services Research and Development, and Departments of Pathology andMolecular Medicine, McMaster University, 1200 Main Street West, Hamilton, Ontario, Canada. sheffiel@mcmaster.ca
authors.forename: W P
authors.initials: WP
authors.lastname: Sheffield
authors.forename: V
authors.initials: V
authors.lastname: Bhakta
authors.forename: B
authors.initials: B
authors.lastname: Trigatti
authors.forename: C
authors.initials: C
authors.lastname: Jenkins
mesh_terms: D001782:Blood Donors; D015925:Cryopreservation; D005260:Female; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood*; D008074:Lipoproteins / Q000097:blood*; D008297:Male; D008875:Middle Aged; D012737:Sex Factors
chemical_list: D008074:Lipoproteins