title: HIV-1 p17 matrix protein interacts with heparan sulfate side chain of CD44v3, syndecan-2, and syndecan-4 proteoglycans expressed on human activated CD4+ T cells affecting tumor necrosis factor alpha and interleukin 2 production.
abstract: HIV-1 p17 contains C- and N-terminal sequences with positively charged residues and a consensus cluster for heparin binding. We have previously demonstrated by affinity chromatography that HIV-1 p17 binds strongly to heparin-agarose at physiological pH and to human activated CD4(+) T cells. In this study we demonstrated that the viral protein binds to heparan sulfate side chains of syndecan-2, syndecan-4, and CD44v3 purified from HeLa cells and that these heparan sulfate proteoglycans (HSPGs) co-localize with HIV-1 p17 on activated human CD4(+) T cells by confocal fluorescence analysis. Moreover, we observed a stimulatory or inhibitory activity when CD4(+) T cells were activated with mitogens together with nanomolar or micromolar concentrations of the matrix protein.
authors.affiliation: Institute of Microbiology, University of Brescia Medical School, Brescia, Italy.
authors.forename: Maria A
authors.initials: MA
authors.lastname: De Francesco
authors.forename: Manuela
authors.initials: M
authors.lastname: Baronio
authors.forename: Claudio
authors.initials: C
authors.lastname: Poiesi
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D005260:Female; D015488:HIV Antigens / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D015497:HIV-1 / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D006367:HeLa Cells; D006497:Heparan Sulfate / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D018960:Hyaluronan Receptors / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D007376:Interleukin-2 / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D008213:Lymphocyte Activation; D008297:Male; D011485:Protein Binding; D053669:Syndecan-2 / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D053671:Syndecan-4 / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D054301:gag Gene Products, Human Immunodeficiency Virus / Q000235:genetics / Q000276:immunology / Q000378:metabolism*
chemical_list: D015488:HIV Antigens; D006497:Heparan Sulfate; D018960:Hyaluronan Receptors; D007376:Interleukin-2; D053669:Syndecan-2; D053671:Syndecan-4; D014409:Tumor Necrosis Factor-alpha; D054301:gag Gene Products, Human Immunodeficiency Virus; C121450:CD44V3,8-10; C508594:IL2 protein, human; C508245:SDC2 protein, human; C508304:SDC4 protein, human; C054858:p17 protein, Human Immunodeficiency Virus Type 1