title: A HPLC-DAD method for the simultaneous determination of five marker components in the traditional herbal medicine Bangpungtongsung-san.
abstract: BACKGROUND
  Bangpungtongsung-san, one of the traditional herbal medicines, was known to be a prescription for obesity.
  
  
  OBJECTIVE
  For the simultaneous determination of five components (paeoniflorin, 6-gingerol, decursin, geniposide, and glycyrrhizin) in Bangpungtongsung-san, a high-performance liquid chromatography with diode-array detector method was established.
  
  
  METHODS
  To develop the method, a reverse phase column, DIONEX C (18) (5 μm, 120 µ, 4.6 mm × 150 mm) was used. The mobile phase consisted of methanol and water using a gradient elution. The UV wavelength was set at 230, 240, and 254 nm. Method validation was accomplished by linearity, precision test, and recovery test.
  
  
  RESULTS
  All calibration curves of components showed good linearity (R (2) > 0.9959). The limit of detection (LOD) and limit of quantification (LOQ) ranged from 0.01 to 0.17 μg/ml and 0.04 to 0.53 μg/ml, respectively. The relative standard deviations (RSD) value of precision test, intraday and interday tests were less than 0.43% and 1.26%. In the recovery test, results of accuracy ranged from 95.27% to 107.70% with RSD values less than 2.21%.
  
  
  CONCLUSIONS
  This developed method was applied to the commercial Bangpungtongsung-san sample and the five marker components were separated effectively without interference of any peaks of components.
authors.affiliation: Department of Biomaterials Engineering, Division of Biotechnology and Bioengineering, Kangwon National University, Chuncheon 200-701, South Korea.
authors.forename: Jin Bae
authors.initials: JB
authors.lastname: Weon
authors.forename: Hye Jin
authors.initials: HJ
authors.lastname: Yang
authors.forename: Jin Yeul
authors.initials: JY
authors.lastname: Ma
authors.forename: Choong Je
authors.initials: CJ
authors.lastname: Ma