title: Glycan microarrays for decoding the glycome.
abstract: In the last decade, glycan microarrays have revolutionized the analysis of the specificity of glycan-binding proteins (GBPs), providing information that simultaneously illuminates the biology mediated by them and decodes the informational content of the glycome. Numerous methods have emerged for arraying glycans in a "chip" format, and glycan libraries have been assembled that address the diversity of the human glycome. Such arrays have been successfully used for analysis of GBPs, which mediate mammalian biology, host-pathogen interactions, and immune recognition of glycans relevant to vaccine production and cancer antigens. This review covers the development of glycan microarrays and applications that have provided insights into the roles of mammalian and microbial GBPs.
authors.affiliation: Department of Chemical Physiology, The Scripps Research Institute, La Jolla, California 92037, USA. rillahan@scripps.edu
authors.forename: Cory D
authors.initials: CD
authors.lastname: Rillahan
authors.forename: James C
authors.initials: JC
authors.lastname: Paulson
mesh_terms: D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D054794:Glycomics / Q000379:methods*; D006801:Humans; D046228:Microarray Analysis / Q000379:methods*; D008969:Molecular Sequence Data; D015394:Molecular Structure; D037121:Plant Lectins / Q000737:chemistry / Q000378:metabolism; D011134:Polysaccharides / Q000032:analysis*; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry / Q000378:metabolism
chemical_list: D037121:Plant Lectins; D011134:Polysaccharides; D011506:Proteins