title: Copolymerization of luminol on screen-printed cells for single-use electrochemiluminescent sensors.
abstract: An efficient electrochemiluminescent (ECL) single-use sensor for H(2)O(2) is presented based on an electropolymerized film prepared on screen-printed gold electrode (gold SPE). A study of the copolymerization of luminol in the presence of different monomers was carried out. The polymeric films were grown potentiodynamically with a potential interval between -0.2 and 1.0 V in 0.2 M H(2)SO(4) and were characterized by their electrochemical, electrochemiluminescent, and superficial features. The polymer with the most efficient growth and ECL emission was poly(luminol-3,3',5,5'-tetramethylbenzidine) at 1:5 ratio. These prepared SPE cells present good mechanical and photoemissive properties. A semi-logarithmic linearization shows a noticeable four decade-width concentration range with a limit of detection (LOD) of 2.6 × 10(-9) M and a precision of 10.2% (n = 5; as relative standard deviation, RSD) in the medium range level. The described SPE ECL sensors will be useful for the determination of oxidase substrates in ECL single-use biosensors.
authors.affiliation: ECSens, Department of Analytical Chemistry, Campus Fuentenueva, Faculty of Sciences, University of Granada, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Ballesta-Claver
authors.forename: M C
authors.initials: MC
authors.lastname: Valencia-Mirón
authors.forename: L F
authors.initials: LF
authors.lastname: Capitán-Vallvey