title: Expression of Notch1 to -4 and their ligands in renal cell carcinoma: a tissue microarray study.
abstract: BACKGROUND
  Mutations in signalling pathways essential for embryonic development often lead to tumourigenesis, as is also true for Notch. The aim of this study was to assess the relationship between Notch1 to -4 and their ligands with anatomopathological features of the patients with renal cell carcinoma (RCC).
  
  
  METHODS
  This study investigated the pattern of protein expression in RCC specimens using tissue microarray technology. A total of 80 paraffin-embedded RCC samples were retrospectively analysed together with ACHN and A.704 cell lines.
  
  
  RESULTS
  Notch1 showed significant positive correlation with chromophobe RCC, no broken capsule, Furhman grade I and when the number of nodes involved was small [(N=1); p=0.039, 0.016, 0.037 and 0.001, respectively)]. Notch3 showed higher expression when the tumour was located in the right kidney (p=0.048).
  
  
  CONCLUSIONS
  Notch1 may be useful in the future as a biomarker for the differential diagnosis of different RCC histological subtypes. Notch1 to -3 may also have potential use as a strong prognostic factor.
authors.affiliation: UDC, Medical Department, A Coruña, Spain.
authors.forename: L M Antón
authors.initials: LM
authors.lastname: Aparicio
authors.forename: V Medina
authors.initials: VM
authors.lastname: Villaamil
authors.forename: G Aparicio
authors.initials: GA
authors.lastname: Gallego
authors.forename: I Santamarina
authors.initials: IS
authors.lastname: Caínzos
authors.forename: R García
authors.initials: RG
authors.lastname: Campelo
authors.forename: L Valbuena
authors.initials: LV
authors.lastname: Rubira
authors.forename: S Vázquez
authors.initials: SV
authors.lastname: Estévez
authors.forename: L León
authors.initials: LL
authors.lastname: Mateos
authors.forename: J L Firvida
authors.initials: JL
authors.lastname: Perez
authors.forename: M Ramos
authors.initials: MR
authors.lastname: Vázquez
authors.forename: O Fernández
authors.initials: OF
authors.lastname: Calvo
authors.forename: M Victoria
authors.initials: MV
authors.lastname: Bolós
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000096:biosynthesis; D002135:Calcium-Binding Proteins / Q000096:biosynthesis; D002292:Carcinoma, Renal Cell / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D036341:Intercellular Signaling Peptides and Proteins / Q000096:biosynthesis; D047908:Intracellular Signaling Peptides and Proteins; D007680:Kidney Neoplasms / Q000378:metabolism* / Q000473:pathology; D008024:Ligands; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis; D008875:Middle Aged; D011379:Prognosis; D040901:Proteomics / Q000379:methods; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis; D051881:Receptor, Notch1 / Q000096:biosynthesis; D051883:Receptor, Notch2 / Q000096:biosynthesis; D000071656:Receptor, Notch3; D000074562:Receptor, Notch4; D051880:Receptors, Notch / Q000096:biosynthesis*; D000072099:Serrate-Jagged Proteins; D046888:Tissue Array Analysis
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D014408:Biomarkers, Tumor; D002135:Calcium-Binding Proteins; C496666:DLL3 protein, human; C424126:DLL4 protein, human; D036341:Intercellular Signaling Peptides and Proteins; D047908:Intracellular Signaling Peptides and Proteins; D008024:Ligands; D008565:Membrane Proteins; C499374:NOTCH3 protein, human; C106075:NOTCH4 protein, human; D011518:Proto-Oncogene Proteins; D051881:Receptor, Notch1; D051883:Receptor, Notch2; D000071656:Receptor, Notch3; D000074562:Receptor, Notch4; D051880:Receptors, Notch; D000072099:Serrate-Jagged Proteins