title: Nonactin hinders intracellular glycosylation in virus-infected baby hamster kidney cells.
abstract: Potent antiviral agents hinder virus-infected cell machinery, leading to rescue from viral damage. In this study, we aimed to identify selective intracellular glycosylation inhibitor(s) that do not suppress glycoprotein synthesis. Our results showed that nonactin is a potent inhibitor of intracellular glycosylation. First, we examined the effects of nonactin on syncytium formation and cytopathic activity in virus-infected baby hamster kidney cells. Nonactin effectively inhibited syncytium formation in a concentration-dependent manner, and infectious virus production was markedly reduced. However, glycoprotein synthesis was not affected. In the presence of 5 µg/ml nonactin, we observed the intracellular accumulation of vesicular stomatitis virus-G protein as well as syncytium formation, but no significant effects on Newcastle disease virus-hemagglutinin-neuramidase glycoprotein synthesis. Our results collectively indicate that nonactin potentially inhibits glycosylation by acting as a suppressor of intracellular glycosylation trafficking.
authors.affiliation: Department of Food and Biotechnology, Hoseo University, Asan 336-795, Korea.
authors.forename: Jin-Man
authors.initials: JM
authors.lastname: Lee
authors.forename: Jong-Guk
authors.initials: JG
authors.lastname: Kim
authors.forename: Tae-Ho
authors.initials: TH
authors.lastname: Kim
authors.forename: Do S
authors.initials: DS
authors.lastname: Lee
authors.forename: Jae H
authors.initials: JH
authors.lastname: Kim
authors.forename: Somi K
authors.initials: SK
authors.lastname: Cho
authors.forename: Key Z
authors.initials: KZ
authors.lastname: Riu
authors.forename: Dong-Sun
authors.initials: DS
authors.lastname: Lee
authors.forename: Sang-Han
authors.initials: SH
authors.lastname: Lee