title: Orally administered Bifidobacterium triggers immune responses following capture by CD11c(+) cells in Peyer's patches and cecal patches.
abstract: We have investigated the immunomodulatory mechanisms of Bifidobacterium pseudocatenulatum JCM7041 (Bp) as model of probiotics following oral administration to mice. This study was conducted with the aim of clarifying the mechanism of immunomodulation induced by oral administration of probiotic bacteria through elucidation of the detailed mechanism of transfer of orally administered bacterial cells within the body and the interaction between bacterial cells and cells of the immune tissues. We observed the localization of Bp in mice following oral administration, showing that Bp was surrounded by CD11c(+) cells in Peyer's patches (PP) and cecal patches (CP). These results indicated that Bp might induce CD11c(+) cell-mediated immune responses directly. Furthermore, IL-10 and IL-12p40 production by Thy1.2(-) cells, including CD11c(+) cells, increased significantly. Production of IL-10 and IL-12p40 by bone marrow-derived dendritic cells (BMDC) was significantly increased by Bp stimulation. These results suggest that oral administration of Bp induces immune responses directly following capture by CD11c(+) dendritic cells (DCs). Subsequently, we observed oral administration of Bp for 1 week induced IgA and IgA-associated cytokine production by CP and PP cells, suggesting that Bp induced DC-mediated immune responses on CP as well as PP.
authors.affiliation: Department of Food Bioscience and Biotechnology, College of Bioresource Sciences, Nihon University, 1866, Kameino, Fujisawa-shi, Kanagawa, 252-0880, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Hiramatsu
authors.forename: Akira
authors.initials: A
authors.lastname: Hosono
authors.forename: Takuma
authors.initials: T
authors.lastname: Konno
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Nakanishi
authors.forename: Masamichi
authors.initials: M
authors.lastname: Muto
authors.forename: Akari
authors.initials: A
authors.lastname: Suyama
authors.forename: Satoshi
authors.initials: S
authors.lastname: Hachimura
authors.forename: Ryuichiro
authors.initials: R
authors.lastname: Sato
authors.forename: Kyoko
authors.initials: K
authors.lastname: Takahashi
authors.forename: Shuichi
authors.initials: S
authors.lastname: Kaminogawa