title: Structural and functional analyses of a saturated acyl ACP thioesterase, type B from immature seed tissue of Jatropha curcas.
abstract: The distinguishing structural and functional domains of plant acyl-acyl carrier protein (ACP) thioesterases and their complex interaction with the ACP-linked fatty acid substrate complex have remained elusive. E. coli based heterologous expression and characterisation of many plant thioesterases reported so far have not been extended and linked to in silico modelling studies to explain the diversity in plant thioesterase substrate specificities. In this study, a thioesterase cDNA isolated from immature seed tissues of Jatropha curcas was found to be type B and specific to stearoyl acyl ACP when expressed in E. coli K27fadD88, a lipid utilisation mutant. Homology modelling and molecular docking of a selected region of the isolated JcFatB protein predicted that it had high affinity towards both stearate (18:0) and palmitate (16:0). Structural analysis of the sequence confirmed the presence of a transit peptide that is processed in multiple steps. The enzyme is localised in the chloroplasts and has an N-terminal inner chloroplast transmembrane domain characteristic of type B plant thioesterases. Docking of ligands with JcFatB and its comparison with a modelled Jatropha thioesterase type A provided further evidence for native substrate preferences of Jatropha thioesterases. This study provides essential clues to develop future methods for large-scale bacterial production of free fatty acids and for design of strategies to modulate the seed oil composition in this important non-edible, seed oil plant.
authors.affiliation: Vittal Mallya Scientific Research Foundation, Bangalore, India.
authors.forename: K G Srikanta
authors.initials: KG
authors.lastname: Dani
authors.forename: K S
authors.initials: KS
authors.lastname: Hatti
authors.forename: P
authors.initials: P
authors.lastname: Ravikumar
authors.forename: A
authors.initials: A
authors.lastname: Kush
mesh_terms: D000595:Amino Acid Sequence; D002736:Chloroplasts / Q000201:enzymology; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D018744:DNA, Plant / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D031286:Jatropha / Q000201:enzymology* / Q000235:genetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019308:Palmitic Acid / Q000378:metabolism; D012639:Seeds / Q000201:enzymology* / Q000235:genetics; D013229:Stearic Acids / Q000378:metabolism; D013379:Substrate Specificity; D013869:Thiolester Hydrolases / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D018076:DNA, Complementary; D018744:DNA, Plant; D013229:Stearic Acids; D019308:Palmitic Acid; C031183:stearic acid; D013869:Thiolester Hydrolases; C018748:oleoyl-(acyl-carrier-protein) hydrolase