title: Spectroscopic studies of amphotericin B-Cu²+ complexes.
abstract: The aim of this research is to investigate amphotericin B (AmB)-Cu(2+) complexes in aqueous solution at different pH values. Electronic absorption, circular dichroism (CD), Raman and FTIR spectroscopies were used in this study. We found that different concentrations of AmB and Cu(2+) ions in solution leads to formation of complexes with stoichiometry of 2:1 and 1:1. The formation of AmB-Cu(2+) complexes at physiological pH values is accompanied by changes of the molecular organization of AmB especially disaggregation. These observed effects might be significant from a medical point of view.
authors.affiliation: Department of Biophysics, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland. mariusz.gagos@up.lublin.pl
authors.forename: Mariusz
authors.initials: M
authors.lastname: Gagoś
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Czernel
authors.forename: Daniel M
authors.initials: DM
authors.lastname: Kamiński
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Kostro
mesh_terms: D000666:Amphotericin B / Q000737:chemistry*; D002942:Circular Dichroism; D003300:Copper / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008968:Molecular Conformation; D009942:Organometallic Compounds / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013059:Spectrum Analysis, Raman
chemical_list: D009942:Organometallic Compounds; D003300:Copper; D000666:Amphotericin B