title: Genotype association of C(-735)T polymorphism of the MMP-2 gene with the risk of carotid atherosclerosis-vulnerable plaque in the Han Chinese population.
abstract: The aim of the current study was to explore the possible association of the polymorphism of C(-735)T in MMP-2 with the vulnerable plaque risk in ultrasound-confirmed carotid atherosclerosis cases. Serum MMP-2 levels were measured to investigate the relationship between the MMP-2 level and the genetic variability. The MMP-2 polymorphism was detected by PCR-RFLP in the 243 cases with stable plaque and 221 cases with vulnerable plaque. Serum MMP-2 levels were measured with ELISA. The results showed that MMP-2 was significantly higher in the cases with vulnerable plaque than in the cases with stable plaque. A statistical difference was found between the genotype distributions in the vulnerable plaque cases and that in the stable cases. T-allele frequency was also found to be over-represented in the stable plaque cases than in the vulnerable plaque cases, which might partially explain the observed difference in the serum MMP-2 levels in the different plaque cases. The current results also suggested that MMP-2 was a risk factor in the cases with vulnerable plaques, whereas TT genotype and T allele might be protective factors in the cases with vulnerable plaques.
authors.affiliation: Department of Neurology, Taizhou Hospital, Wenzhou Medical College, Taizhou, Zhejiang, 317000 PR China.
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Jin
authors.forename: Min
authors.initials: M
authors.lastname: Zhu
authors.forename: Xian-Fang
authors.initials: XF
authors.lastname: Lin
authors.forename: Xiao-Fei
authors.initials: XF
authors.lastname: Hu
authors.forename: Wan-Fen
authors.initials: WF
authors.lastname: Wang
authors.forename: Zhao
authors.initials: Z
authors.lastname: Han
authors.forename: Ling-Zhi
authors.initials: LZ
authors.lastname: Huang
mesh_terms: D000368:Aged; D044466:Asian People / Q000235:genetics*; D002340:Carotid Artery Diseases / Q000208:ethnology / Q000235:genetics* / Q000503:physiopathology; D002681:China; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006439:Hemodynamics; D006801:Humans; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000235:genetics* / Q000378:metabolism; D058226:Plaque, Atherosclerotic / Q000208:ethnology / Q000235:genetics* / Q000503:physiopathology; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
chemical_list: C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2