title: Acute exposure to glyphosate herbicide affects oxidative parameters in piava (Leporinus obtusidens).
abstract: In recent years, commercial glyphosate herbicide formulations have been widely used in agriculture to control aquatic weeds. These pesticides may result in disruption of ecological balance, causing damage to nontarget organisms including fish. Teleostean fish (Leporinus obtusidens) were exposed to commercial glyphosate herbicide formulation at 0 (control), 3, 6, 10 or 20 mg L(-1) for 96 h. The effects of herbicide on plasmatic metabolic parameters, thiobarbituric acid reactive substances (TBARS), catalase activity, protein carbonyl, and mucus layer parameters were studied. Plasmatic glucose and lactate levels increased but protein levels showed reduction after herbicide exposure. TBARS levels in brain showed a reduction at all tested concentrations. However, liver demonstrated increased TBARS levels at all tested concentrations, whereas in white muscle TBARS production did not change after exposure to herbicide. Fish exposed to all concentrations of glyphosate showed increase in liver catalase activity and protein carbonyl. Herbicide exposure increased protein and carbohydrate levels of the mucus layer at all tested concentrations. The present results showed that, in 96 h, glyphosate changed toxicological parameters analyzed in piava. Parameters measured in this study may be useful in environmental biomonitoring.
authors.affiliation: Adaptive Biochemistry Laboratory, Department of Chemistry, Federal University of Santa Maria, RS, Brazil. lissag@ufmt.br
authors.forename: Lissandra
authors.initials: L
authors.lastname: Glusczak
authors.forename: Vania Lucia
authors.initials: VL
authors.lastname: Loro
authors.forename: Alexandra
authors.initials: A
authors.lastname: Pretto
authors.forename: Bibiana Silveira
authors.initials: BS
authors.lastname: Moraes
authors.forename: Alice
authors.initials: A
authors.lastname: Raabe
authors.forename: Marta Frescura
authors.initials: MF
authors.lastname: Duarte
authors.forename: Milene Braga
authors.initials: MB
authors.lastname: da Fonseca
authors.forename: Charlene Cavalheiro
authors.initials: CC
authors.lastname: de Menezes
authors.forename: Dênia Mendes de Sousa
authors.initials: DM
authors.lastname: Valladão
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D004784:Environmental Monitoring / Q000379:methods; D005260:Female; D005399:Fishes / Q000378:metabolism*; D005998:Glycine / Q000031:analogs & derivatives* / Q000633:toxicity; D006540:Herbicides / Q000633:toxicity*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D018656:Muscle Fibers, Fast-Twitch / Q000187:drug effects / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D023382:Toxicity Tests, Acute; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D000097797:Glyphosate
chemical_list: D005998:Glycine; D006540:Herbicides; D017392:Thiobarbituric Acid Reactive Substances; D014874:Water Pollutants, Chemical; D000097797:Glyphosate