title: Role of quercetin on PCBs (Aroclor-1254) induced impairment of dopaminergic receptor mRNA expression in cerebral cortex of adult male rats.
abstract: The present study is aimed to evaluate the putative neuroprotective effect of quercetin on PCB induced impairment of dopaminergic receptor mRNA expression in cerebral cortex of adult male Wistar rats. Group I (control) received only vehicle (corn oil; 0.1 ml/kg bwt) intraperitoneally (i.p); Group II Aroclor 1254 at a dose of 2 mg/kg bwt/day (i.p); Group III (Aroclor 1254-exposed (i.p), quercetin treated gavage (50 mg/kg bwt/day); Group IV received quercetin alone (gavage). 24 h after the 30th day treatment rats were euthanized. From each rat cerebral cortex tissues was collected and analyzed for mean activities of creatine kinase, acetylcholine esterase, Na(+)/K(+), Ca(2+) and Mg(2+)ATPases, Hydrogen peroxide generation, protein carbonyl content and lipid peroxidation levels. The fates of the mRNA expression of dopaminergic receptors, Cacna1d on all the groups were studied by RT-PCR. Results evidenced that significant reduction of neurodegeneration in PCBs exposed rats treated with quercetin was ascertained suggesting, quercetin treatment precludes against PCB induced oxidative stress and protects dopaminergic receptor dysfunction in rat cerebral cortex.
authors.affiliation: Department of Biotechnology, Bharathidasan University, Trichy, 620024, India.
authors.forename: Rasiah
authors.initials: R
authors.lastname: Pratheepa Kumari
authors.forename: Kandaswamy
authors.initials: K
authors.lastname: Selvakumar
authors.forename: Senthamilselvan
authors.initials: S
authors.lastname: Bavithra
authors.forename: Rafiq
authors.initials: R
authors.lastname: Zumaana
authors.forename: Gunasekaran
authors.initials: G
authors.lastname: Krishnamoorthy
authors.forename: Jagadeesan
authors.initials: J
authors.lastname: Arunakaran
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D015220:Calcium Channels / Q000235:genetics / Q000378:metabolism; D002540:Cerebral Cortex / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D020111:Chlorodiphenyl (54% Chlorine) / Q000494:pharmacology*; D003402:Creatine Kinase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D018696:Neuroprotective Agents / Q000494:pharmacology; D016877:Oxidants / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects; D011078:Polychlorinated Biphenyls / Q000494:pharmacology*; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D011794:Quercetin / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D011954:Receptors, Dopamine / Q000235:genetics* / Q000378:metabolism
chemical_list: D000975:Antioxidants; D015220:Calcium Channels; D018696:Neuroprotective Agents; D016877:Oxidants; D020033:Protein Isoforms; D012333:RNA, Messenger; D011954:Receptors, Dopamine; D020111:Chlorodiphenyl (54% Chlorine); C093712:Cacna1d protein, rat; D011794:Quercetin; D006861:Hydrogen Peroxide; D011078:Polychlorinated Biphenyls; D003402:Creatine Kinase; D000110:Acetylcholinesterase; D000251:Adenosine Triphosphatases