title: Adenosine involvement on bronchial reactivity modulation by diesel exhaust.
abstract: In recent decades, epidemiologic investigations have suggested a strong relationship between air pollution and an increase in the prevalence of allergic rhinitis and asthma.
  
  
  OBJECTIVE
  To investigate the possible involvement of adenosine (AD) in bronchomotor effects of diesel exhaust (DE).
  
  
  METHODS
  Isolated bronchi from ovalbumin (OVA) sensitized rats were challenged in presence or absence of diesel exhaust extract (DEE). AD was delivered on organ bath before or after DEE, at concentrations did not produce significantly contractile effects.
  
  
  RESULTS
  AD (0.1 microM) pre-treatment increased bronchomotor effects of DEE: amplified the bronchoconstrictor effects of OVA with more than 35% and decreased Emax of terbutaline induced bronchorelaxation of acetylcholine (Ach) preconstricted bronchial rings (up to 20%), but did not significantly modify ACh-induced contractions. OVA-induced contractions, ACh-induced contractions and terbutaline-induced relaxations have not been significantly modified as compare with DEE alone. On the other hand, DEE amplified AD (cumulative doses) contractile effects.
  
  
  CONCLUSIONS
  These results confirmed our initial hypothesis that AD could partial mediate or at least, modulate DEE effects on airway reactivity.
authors.affiliation: Gr.T. Popa University of Medicine and Pharmacy Iaşi, School of Medicine, Department of Physiology.
authors.forename: Elena
authors.initials: E
authors.lastname: Cojocaru
authors.forename: Irina Luciana
authors.initials: IL
authors.lastname: Dumitriu
authors.forename: B
authors.initials: B
authors.lastname: Gurzu
authors.forename: Ioana Stefania
authors.initials: IS
authors.lastname: Ciureanu
authors.forename: Maria
authors.initials: M
authors.lastname: Dinca
authors.forename: M
authors.initials: M
authors.lastname: Costuleanu
authors.forename: Simona Mihaela
authors.initials: SM
authors.lastname: Slătineanu
authors.forename: Brigitte
authors.initials: B
authors.lastname: Scutaru
authors.forename: Gh
authors.initials: G
authors.lastname: Petrescu
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000241:Adenosine / Q000494:pharmacology*; D000393:Air Pollutants / Q000009:adverse effects*; D000465:Algorithms; D000818:Animals; D001980:Bronchi / Q000187:drug effects*; D016535:Bronchial Hyperreactivity / Q000139:chemically induced* / Q000503:physiopathology*; D001993:Bronchodilator Agents / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D010047:Ovalbumin; D051381:Rats; D017208:Rats, Wistar; D013726:Terbutaline / Q000494:pharmacology; D014665:Vasodilator Agents / Q000494:pharmacology*; D001335:Vehicle Emissions
chemical_list: D000393:Air Pollutants; D001993:Bronchodilator Agents; D014665:Vasodilator Agents; D001335:Vehicle Emissions; D010047:Ovalbumin; D000241:Adenosine; D013726:Terbutaline; D000109:Acetylcholine