title: Feasibility of the Ussing chamber technique for the determination of in vitro jejunal permeability of passively absorbed compounds in different animal species.
abstract: The aim of this study was to assess the feasibility of the Ussing chamber technique for the determination of the jejunal permeability of passively absorbed, high permeability model compounds (acetaminophen and ketoprofen) in different animal species. Additionally, electrophysiological measurements and histological examination of pre- and post-incubation tissue specimens were performed. Apparent permeability coefficients of turkey and dog jejunum were low and highly variable due to tissue fragility caused by differences in thickness of the remaining intestinal layers after stripping and resulting in severe damage. Pig and horse jejunum were markedly more suitable for permeability determinations and mild signs of deterioration were noticed after 120 min of incubation. Transepithelial electrical resistance and potential difference did not correlate well with the observed tissue damage. From these data, the Ussing chamber technique appears to allow for permeability measurements within a species, but seems unsuitable for interspecies permeability comparison. However, further validation of the method with low permeability compounds and actively transported compounds is needed.
authors.affiliation: Department of Pharmacology, Toxicology and Biochemistry, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium. Eva.Neirinckx@UGent.be
authors.forename: E
authors.initials: E
authors.lastname: Neirinckx
authors.forename: C
authors.initials: C
authors.lastname: Vervaet
authors.forename: J
authors.initials: J
authors.lastname: Michiels
authors.forename: S
authors.initials: S
authors.lastname: De Smet
authors.forename: W
authors.initials: W
authors.lastname: Van den Broeck
authors.forename: J P
authors.initials: JP
authors.lastname: Remon
authors.forename: P
authors.initials: P
authors.lastname: De Backer
authors.forename: S
authors.initials: S
authors.lastname: Croubels
mesh_terms: D000082:Acetaminophen / Q000493:pharmacokinetics*; D018712:Analgesics, Non-Narcotic / Q000493:pharmacokinetics*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000662:veterinary; D015194:Diffusion Chambers, Culture / Q000295:instrumentation / Q000379:methods / Q000662:veterinary*; D004285:Dogs; D017097:Electric Impedance; D005240:Feasibility Studies; D005260:Female; D006736:Horses; D066298:In Vitro Techniques; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000033:anatomy & histology / Q000378:metabolism* / Q000502:physiology; D007583:Jejunum / Q000033:anatomy & histology / Q000378:metabolism* / Q000502:physiology; D007660:Ketoprofen / Q000493:pharmacokinetics*; D008297:Male; D008564:Membrane Potentials; D010539:Permeability; D013552:Swine; D014422:Turkeys
chemical_list: D018712:Analgesics, Non-Narcotic; D000082:Acetaminophen; D007660:Ketoprofen