title: Stimulation of homology-directed repair at I-SceI-induced DNA breaks during the permissive life cycle of human cytomegalovirus.
abstract: Human cytomegalovirus (HCMV) selectively relocalizes many DNA repair proteins, thereby avoiding a potentially detrimental damage response. In the present study, we evaluated interactions between HCMV and the homology-directed repair (HDR) pathway. In permissive human foreskin fibroblasts, a fluorescence-based double-stranded break repair assay was used to determine that HCMV stimulated HDR. Repair of both stably integrated and extrachromosomal reporter substrates was observed to increase. HDR was also stimulated through individual expression of the viral immediate-early protein IE1-72, mimicking full virus infection. These experiments further demonstrate HCMV's role in modulating critical cellular processes during a permissive infection.
authors.affiliation: Department of Biological Sciences and Center for Reproductive Biology, University of Idaho, Moscow, ID 83844-3051, USA.
authors.forename: A S
authors.initials: AS
authors.lastname: Kulkarni
authors.forename: E A
authors.initials: EA
authors.lastname: Fortunato
mesh_terms: D002460:Cell Line; D003587:Cytomegalovirus / Q000235:genetics / Q000502:physiology*; D053903:DNA Breaks, Double-Stranded; D004260:DNA Repair / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D052241:Fanconi Anemia Complementation Group G Protein / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism / Q000821:virology*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D017874:Immediate-Early Proteins / Q000378:metabolism*; D051135:Rad51 Recombinase / Q000378:metabolism; D014779:Virus Replication
chemical_list: D004268:DNA-Binding Proteins; C499909:FANCG protein, human; D052241:Fanconi Anemia Complementation Group G Protein; C088645:IE1 protein, cytomegalovirus; D017874:Immediate-Early Proteins; C448305:RAD51C protein, human; D049452:Green Fluorescent Proteins; C495153:RAD51 protein, human; D051135:Rad51 Recombinase; D015252:Deoxyribonucleases, Type II Site-Specific