title: The efficacy of a novel antibacterial hydroxyapatite nanoparticle-coated indwelling urinary catheter in preventing biofilm formation and catheter-associated urinary tract infection in rabbits.
abstract: The aim of this study was to investigate of the efficacy and reliability of a novel antimicrobial hydroxyapatite (HA) nanoparticle coating of urethral catheters, in the prophylaxis of biofilm formation and bacteriuria in rabbits. A total of 60 male rabbits were randomized to the control and study groups and each group was divided into three subgroups depending on 3, 5 and 7 days of the urethral catheterization period. The rabbits in the study group were catheterized with Ag(+)-incorporated nano-HA coated urethral catheters and those in the control group with standard silicon-latex urethral catheters. Urine and catheter surface smear samples were conducted for bacteriological analysis. Catheter cross-section samples were undergone measuring of biofilm thickness. Tissue samples of bladder and urethra were inspected for histological changes. The results indicate that at the end of 7 days of the catheterization period, the number of the rabbits with bacteriuria was significantly lower in the study group versus control group (p (¶) = 0.020). The biofilm formation on luminal surface of the catheters was significantly thinner in the study group versus control group, at the end of 5 and 7 days of the catheterization period (0.035 and 0.035, respectively).No histological adverse change or particle penetration was detected in the urothelium. In conclusion, it was observed that Ag(+) + HA nanoparticle coating significantly lowered the incidence of catheter-related bacteriuria and decreased biofilm formation, at the end of 7 days study period. The novel antimicrobial urethral catheter coating appeared to have a potential in the prophylaxis of catheter-induced urinary tract infections.
authors.affiliation: Department of Urology, Adana Numune Training and Research Hospital, Adana, Turkey. yalcinevli@yahoo.com
authors.forename: Yalçın
authors.initials: Y
authors.lastname: Evliyaoğlu
authors.forename: Murat
authors.initials: M
authors.lastname: Kobaner
authors.forename: Hande
authors.initials: H
authors.lastname: Celebi
authors.forename: Kazım
authors.initials: K
authors.lastname: Yelsel
authors.forename: Aydın
authors.initials: A
authors.lastname: Doğan
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology / Q000627:therapeutic use*; D001437:Bacteriuria / Q000453:epidemiology / Q000517:prevention & control; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development*; D055499:Catheter-Related Infections / Q000453:epidemiology / Q000517:prevention & control*; D002408:Catheters, Indwelling / Q000009:adverse effects / Q000382:microbiology*; D017886:Durapatite; D015994:Incidence; D008297:Male; D023421:Models, Animal; D053758:Nanoparticles; D010865:Pilot Projects; D011817:Rabbits; D015203:Reproducibility of Results; D014552:Urinary Tract Infections / Q000453:epidemiology / Q000517:prevention & control*
chemical_list: D000900:Anti-Bacterial Agents; D017886:Durapatite