title: Partially purified extract and viscolin from Viscum coloratum attenuate airway inflammation and eosinophil infiltration in ovalbumin-sensitized mice.
abstract: OBJECTIVE
  Viscum coloratum Nakai is used in traditional Chinese medicine to treat various diseases, including hemorrhage, hypertension, and inflammatory diseases. A previous study demonstrated a partially purified extract (PPE-SVC) and viscolin from Viscum coloratum Nakai inhibited phosphodiesterase activity. In this study, we evaluated the anti-asthmatic effects of PPE-SVC and viscolin, from Viscum coloratum Nakai, in OVA-sensitized mice.
  
  
  METHODS
  Female BALB/c mice were sensitized and challenged with ovalbumin (OVA). The mice were randomized into groups and treated with PPE-SVC, viscolin, or rolipram by intraperitoneal injection on 1h before each inhalation of OVA and airway hyperresponsiveness (AHR).
  
  
  RESULTS
  PPE-SVC and viscolin suppressed AHR and reduced eosinophil infiltration of the lungs in OVA-sensitized mice. Moreover, PPE-SVC and viscolin inhibited chemokines, including CCL11 and CCL24, and Th2-associated cytokines in bronchoalveolar lavage fluid. However, PPE-SVC and viscolin could not decrease IL-4, IL-5, and IL-13 levels in cultures of OVA-activated spleen cells.
  
  
  CONCLUSIONS
  PPE-SVC and viscolin attenuate airway inflammation and eosinophil infiltration in OVA-sensitized mice.
authors.affiliation: School of Traditional Chinese Medicine, Chang Gung University, Taoyuan, Taiwan.
authors.forename: Jiann-Jong
authors.initials: JJ
authors.lastname: Shen
authors.forename: Ming-Shan
authors.initials: MS
authors.lastname: Chiang
authors.forename: Ming-Ling
authors.initials: ML
authors.lastname: Kuo
authors.forename: Yann-Lii
authors.initials: YL
authors.lastname: Leu
authors.forename: Tsong-Long
authors.initials: TL
authors.lastname: Hwang
authors.forename: Chian-Jiun
authors.initials: CJ
authors.lastname: Liou
authors.forename: Wen-Chung
authors.initials: WC
authors.lastname: Huang
mesh_terms: D000818:Animals; D018927:Anti-Asthmatic Agents / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D001713:Biphenyl Compounds / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D016535:Bronchial Hyperreactivity / Q000276:immunology / Q000378:metabolism / Q000517:prevention & control*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D018925:Chemokines / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D004804:Eosinophils / Q000378:metabolism*; D005260:Female; D007249:Inflammation / Q000276:immunology / Q000378:metabolism / Q000517:prevention & control; D008168:Lung / Q000187:drug effects / Q000276:immunology; D008516:Medicine, Chinese Traditional; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D011407:Propane / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D011897:Random Allocation; D020889:Rolipram; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D028181:Viscum / Q000737:chemistry*
chemical_list: D018927:Anti-Asthmatic Agents; D000893:Anti-Inflammatory Agents; D001713:Biphenyl Compounds; D018925:Chemokines; D016207:Cytokines; D010936:Plant Extracts; C513809:viscolin; D010047:Ovalbumin; D020889:Rolipram; D011407:Propane