title: Apigetrin induces erythroid differentiation of human leukemia cells K562: proteomics approach.
abstract: METHODS
  Induction of cancer-cell differentiation is an alternative approach for cancer chemotherapy. There are numerous studies that diets containing an abundance of fruits and vegetables have protection against cancers, and the main agents thought to provide such protective effect are flavonoids. In this study we used apigetrin as a possible cell differentiation inducer and chronic leukemia cells K562 for their pluripotent differentiating potency.
  
  
  RESULTS
  Prolonged treatment with 75 μM apigetrin induced erythroid differentiation of K562 cells with specific marker glycophorin A expression and fetal hemoglobin synthesis in treated cells, which was accompanied with G(2) /M arrest. Proteomics data revealed the downregulation of several proteins expression involved in cell cycle regulation, protein synthesis and nuclear import and export of signaling molecules.
  
  
  CONCLUSIONS
  This is the first evidence that natural compound apigetrin may induce cancer cell differentiation thus could be one of the possible explanations of its antitumor effects.
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki, Japan.
authors.forename: Soninkhishig
authors.initials: S
authors.lastname: Tsolmon
authors.forename: Eri
authors.initials: E
authors.lastname: Nakazaki
authors.forename: Junkyu
authors.initials: J
authors.lastname: Han
authors.forename: Hiroko
authors.initials: H
authors.lastname: Isoda
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D047310:Apigenin / Q000494:pharmacology*; D002453:Cell Cycle; D002454:Cell Differentiation / Q000187:drug effects*; D002470:Cell Survival; D015536:Down-Regulation; D005319:Fetal Hemoglobin / Q000096:biosynthesis; D006021:Glycophorins / Q000096:biosynthesis; D006801:Humans; D020014:K562 Cells; D007938:Leukemia / Q000473:pathology*; D040901:Proteomics / Q000379:methods
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D006021:Glycophorins; C057792:apigetrin; D047310:Apigenin; D005319:Fetal Hemoglobin