title: Deletion of SNAP-23 results in pre-implantation embryonic lethality in mice.
abstract: SNARE-mediated membrane fusion is a pivotal event for a wide-variety of biological processes. SNAP-25, a neuron-specific SNARE protein, has been well-characterized and mouse embryos lacking Snap25 are viable. However, the phenotype of mice lacking SNAP-23, the ubiquitously expressed SNAP-25 homolog, remains unknown. To reveal the importance of SNAP-23 function in mouse development, we generated Snap23-null mice by homologous recombination. We were unable to obtain newborn SNAP-23-deficient mice, and analysis of pre-implantation embryos from Snap23(Δ/wt) matings revealed that Snap23-null blastocysts were dying prior to implantation at embryonic day E3.5. Thus these data reveal a critical role for SNAP-23 during embryogenesis.
authors.affiliation: Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, United States of America.
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Suh
authors.forename: Aki
authors.initials: A
authors.lastname: Yoshimoto-Furusawa
authors.forename: Karis A
authors.initials: KA
authors.lastname: Weih
authors.forename: Lino
authors.initials: L
authors.lastname: Tessarollo
authors.forename: Katherine W
authors.initials: KW
authors.lastname: Roche
authors.forename: Susan
authors.initials: S
authors.lastname: Mackem
authors.forename: Paul A
authors.initials: PA
authors.lastname: Roche
mesh_terms: D000483:Alleles; D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000378:metabolism; D001947:Breeding; D016923:Cell Death; D010064:Embryo Implantation; D020964:Embryo Loss / Q000378:metabolism* / Q000473:pathology*; D005260:Female; D017353:Gene Deletion; D018390:Gene Targeting; D006579:Heterozygote; D051379:Mice; D008810:Mice, Inbred C57BL; D011247:Pregnancy; D050766:Qb-SNARE Proteins / Q000172:deficiency* / Q000378:metabolism; D050767:Qc-SNARE Proteins / Q000172:deficiency* / Q000378:metabolism
chemical_list: D050766:Qb-SNARE Proteins; D050767:Qc-SNARE Proteins; C107017:Snap23 protein, mouse