title: Real-time measurements of protein dynamics using fluorescence activation-coupled protein labeling method.
abstract: We present a fluorescence activation-coupled protein labeling (FAPL) method, which employs small-molecular probes that exhibit almost no basal fluorescence but acquire strong fluorescence upon covalent binding to tag-proteins. This method enables real-time imaging of protein labeling without any washout process and is uniquely suitable for real-time imaging of protein dynamics on the cell surface. We applied this method to address the spatiotemporal dynamics of the EGF receptor during cell migration.
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1, Hongo, Tokyo, 113-0033, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Komatsu
authors.forename: Kai
authors.initials: K
authors.lastname: Johnsson
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Okuno
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Bito
authors.forename: Takanari
authors.initials: T
authors.lastname: Inoue
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Nagano
authors.forename: Yasuteru
authors.initials: Y
authors.lastname: Urano
mesh_terms: D000818:Animals; D019556:COS Cells; D002460:Cell Line; D002465:Cell Movement; D002522:Chlorocebus aethiops; D066246:ErbB Receptors / Q000032:analysis; D005456:Fluorescent Dyes / Q000737:chemistry*; D015336:Molecular Probe Techniques; D015335:Molecular Probes / Q000737:chemistry*; D011506:Proteins / Q000032:analysis*
chemical_list: D005456:Fluorescent Dyes; D015335:Molecular Probes; D011506:Proteins; D066246:ErbB Receptors