title: Estimation of cell number based on metabolic activity: the MTT reduction assay.
abstract: The MTT reduction assay is used to determine the level of metabolic activity in eukaryotic cells, -including animal, plant, and fungal cells. If the metabolic rate is constant, the technique can be employed to count living cells in a sample. Once it is set up, the method is very robust, and can be automatized to be applied on a large number of samples.
authors.affiliation: Genetics Unit, Shriners Hospital for Children, Montreal, QC, Canada. koopac@gmail.com
authors.forename: László
authors.initials: L
authors.lastname: Kupcsik
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000379:methods*; D019556:COS Cells; D002452:Cell Count / Q000379:methods*; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D010084:Oxidation-Reduction; D013778:Tetrazolium Salts / Q000737:chemistry / Q000378:metabolism*
chemical_list: D013778:Tetrazolium Salts