title: Hypertension in response to AT1-AA: role of reactive oxygen species in pregnancy-induced hypertension.
abstract: BACKGROUND
  Agonistic autoantibodies to the angiotensin II type I receptor (AT1-AA) and reactive oxygen species (ROS) are implicated in the pathophysiology of preeclampsia. The objective of this study was to determine the role of AT1-AA to stimulate placental oxidative stress in vivo and role ROS in mediating hypertension in response to AT1-AA during pregnancy.
  
  
  METHODS
  To achieve these goals, blood pressure (mean arterial pressure (MAP)) and ROS were analyzed in AT1-AA-induced hypertensive pregnant rats in the presence and absence of a superoxide dismutase mimetic, tempol. Rat AT1-AA (1:50) and tempol (30 mg/kg/day) were administered to pregnant rats beginning on day 12 of gestation. On day 19, MAP was analyzed and tissues collected for ROS analysis via lucigenin chemiluminescence.
  
  
  RESULTS
  MAP increased from 101 ± 2 normal pregnant (NP) rats to 116 ± 2 mm Hg in chronic AT1-AA infused rats (P = 0.002). Placental basal and NADPH oxidase stimulated ROS was 29 ± 6 and 92 ± 10 relative light units (RLUs) in NP rats. These levels increased to 159 ± 29 (P < 0.0001) and 287 ± 60 RLUs (P < 0.006) in AT1-AA infused rats. MAP in AT1-AA + tempol rats was 109 ± 2 mm Hg, no difference than tempol-treated controls (109 ± 3 mm Hg). Administration of tempol decreased basal and NADPH-stimulated placental ROS in AT1-AA-treated rats (121 ± 13; 262 ± 21 RLUs). Basal and NADPH-stimulated ROS in tempol-treated controls were 69 ± 24; 141 ± 33 RLUs.
  
  
  CONCLUSIONS
  This study indicates that AT1-AA's contribute to placental oxidative stress; one mechanism whereby the AT1-AA mediates hypertension during pregnancy.
authors.affiliation: Department of Obstetrics and Gynecology, University of Mississippi Medical Center, Jackson, Mississippi, USA.
authors.forename: Marc R
authors.initials: MR
authors.lastname: Parrish
authors.forename: Kedra
authors.initials: K
authors.lastname: Wallace
authors.forename: Kiran B
authors.initials: KB
authors.lastname: Tam Tam
authors.forename: Florian
authors.initials: F
authors.lastname: Herse
authors.forename: Abram
authors.initials: A
authors.lastname: Weimer
authors.forename: Katrin
authors.initials: K
authors.lastname: Wenzel
authors.forename: Gerd
authors.initials: G
authors.lastname: Wallukat
authors.forename: Lillian F
authors.initials: LF
authors.lastname: Ray
authors.forename: Marrietta
authors.initials: M
authors.lastname: Arany
authors.forename: Kathy
authors.initials: K
authors.lastname: Cockrell
authors.forename: James N
authors.initials: JN
authors.lastname: Martin
authors.forename: Ralf
authors.initials: R
authors.lastname: Dechend
authors.forename: Babbette
authors.initials: B
authors.lastname: LaMarca
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001323:Autoantibodies / Q000502:physiology*; D001794:Blood Pressure / Q000187:drug effects / Q000502:physiology; D003497:Cyclic N-Oxides / Q000494:pharmacology; D005260:Female; D046110:Hypertension, Pregnancy-Induced / Q000378:metabolism* / Q000503:physiopathology*; D023421:Models, Animal; D019255:NADPH Oxidases / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D010920:Placenta / Q000378:metabolism; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism*; D044140:Receptor, Angiotensin, Type 1 / Q000276:immunology*; D013113:Spin Labels
chemical_list: D000975:Antioxidants; D001323:Autoantibodies; D003497:Cyclic N-Oxides; D017382:Reactive Oxygen Species; D044140:Receptor, Angiotensin, Type 1; D013113:Spin Labels; D019255:NADPH Oxidases; C001803:tempol