title: A zinc finger protein Tsip1 controls Cucumber mosaic virus infection by interacting with the replication complex on vacuolar membranes of the tobacco plant.
abstract: • In Cucumber mosaic virus (CMV) RNA replication, replicase-associated protein CMV 1a and RNA-dependent RNA polymerase protein CMV 2a are essential for formation of an active virus replicase complex on vacuolar membranes. • To identify plant host factors involved in CMV replication, a yeast two-hybrid system was used with CMV 1a protein as bait. One of the candidate genes encoded Tsi1-interacting protein 1 (Tsip1), a zinc (Zn) finger protein. Tsip1 strongly interacted with CMV 2a protein, too. • Formation of a Tsip1 complex involving CMV 1a or CMV 2a was confirmed in vitro and in planta. When 35S::Tsip1 tobacco (Nicotiana tabacum) plants were inoculated with CMV-Kor, disease symptom development was delayed and the accumulation of CMV RNAs and coat protein was decreased in both the infected local leaves and the uninfected upper leaves, compared with the wild type, whereas Tsip1-RNAi plants showed modestly but consistently increased CMV susceptibility. In a CMV replication assay, CMV RNA concentrations were reduced in the 35S::Tsip1 transgenic protoplasts compared with wild-type (WT) protoplasts. • These results indicate that Tsip1 might directly control CMV multiplication in tobacco plants by formation of a complex with CMV 1a and CMV 2a.
authors.affiliation: School of Life Sciences and Biotechnology, Korea University, 1, 5-ga, Anam-dong, Sungbuk-gu, Seoul 136-701, Republic of Korea.
authors.forename: Sung Un
authors.initials: SU
authors.lastname: Huh
authors.affiliation: Examination Division of Food and Biological Resources, Korean Intellectual Property Office, Daejeon 302-701, Republic of Korea.
authors.forename: Min Jung
authors.initials: MJ
authors.lastname: Kim
authors.affiliation: Section of Plant Biology, College of Biological Sciences, University of California, Davis, CA 95616, USA.
authors.forename: Byung-Kook
authors.initials: BK
authors.lastname: Ham
authors.affiliation: School of Life Sciences and Biotechnology, Korea University, 1, 5-ga, Anam-dong, Sungbuk-gu, Seoul 136-701, Republic of Korea.
authors.forename: Kyung-Hee
authors.initials: KH
authors.lastname: Paek
mesh_terms: D017799:Cucumovirus / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D007425:Intracellular Membranes / Q000378:metabolism / Q000821:virology; D008780:Methyltransferases / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D010641:Phenotype; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D025941:Protein Interaction Mapping; D011523:Protoplasts; D034622:RNA Interference; D012367:RNA, Viral / Q000096:biosynthesis*; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins; D014026:Nicotiana / Q000235:genetics / Q000502:physiology* / Q000821:virology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D014617:Vacuoles / Q000378:metabolism / Q000821:virology; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D014779:Virus Replication / Q000502:physiology; D016335:Zinc Fingers / Q000235:genetics; D001616:beta-Galactosidase
chemical_list: D004268:DNA-Binding Proteins; D010940:Plant Proteins; D012367:RNA, Viral; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; C432450:Tsi1 protein, Nicotiana tabacum; D014764:Viral Proteins; C508104:1a protein, cucumber mosaic virus; D008780:Methyltransferases; D012324:RNA-Dependent RNA Polymerase; D001616:beta-Galactosidase