title: Glutathione S-transferase M1 and T1 polymorphisms in Turkish patients with varicocele.
abstract: Several studies have shown that high oxidative stress levels are associated with varicocele. The GST (glutathione S-transferase) family of genes is critical in the protection of cells from oxidative stress because they utilise as substrates a wide variety of products of oxidative stress. The objective of this study was to assess the relationship between genetic polymorphism in GST-M1 and GST-T1 and varicocele using 109 varicocele patients and 123 controls. Varicoceles were clinically graded as Grade 1, Grade 2 and Grade 3. GST-M1 and GST-T1 genes were determined by polymerase chain reaction. Although the GST-M1 null genotype was higher in Grade 3 than in Grade 1, 2 and controls, there were no statistical differences between control group and varicocele groups according to GST-M1 and GST-T1 null genotype. Men with varicocele do not have more GST-M1 and GST-T1 null polymorphisms than men without varicocele. Additional studies are needed to assess the exact mechanism by which the varicocele corresponds to elevated ROS levels.
authors.affiliation: Department of Genetics, Selcuk University Meram Medical School, Konya, Turkey.
authors.forename: H
authors.initials: H
authors.lastname: Acar
authors.forename: M
authors.initials: M
authors.lastname: Kılınç
authors.forename: S
authors.initials: S
authors.lastname: Guven
authors.forename: Z
authors.initials: Z
authors.lastname: Inan
mesh_terms: D001483:Base Sequence; D016022:Case-Control Studies; D017931:DNA Primers / Q000235:genetics; D056726:Genetic Association Studies; D005982:Glutathione Transferase / Q000172:deficiency / Q000235:genetics*; D006801:Humans; D008297:Male; D018384:Oxidative Stress / Q000235:genetics; D011110:Polymorphism, Genetic; D014421:Turkey; D014646:Varicocele / Q000201:enzymology* / Q000235:genetics*
chemical_list: D017931:DNA Primers; D005982:Glutathione Transferase; C413545:glutathione S-transferase T1