title: Determination of aflatoxins in animal feeds by liquid chromatography/tandem mass spectrometry with isotope dilution.
abstract: The objective of the present study is to develop a simple, fast method for detection of aflatoxins in animal feeds. Simultaneous quantitation of four aflatoxins (AFB(1), AFB(2), AFG(1) and AFG(2)) in animal feeds was achieved in a single liquid chromatography/tandem mass spectrometry (LC/MS/MS) run. The solid-phase extraction cleanup step is eliminated with the stable isotope dilution method. Matrix effects were observed and overcome by isotope dilution. The method was tested in a variety of animal feed matrices and proved to be accurate and reliable. Method ruggedness tests resulted in recoveries of 78% to 122% with an intra-day assay precision of 2% to 15% and an inter-day assay precision of 3% to 17%. These results indicate that this method is suitable for quantitation of aflatoxins in animal feeds.
authors.affiliation: Office of the Texas State Chemist, Texas AgriLife Research, Texas A&M University, College Station, TX 77843, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Herrman
authors.forename: Susie Y
authors.initials: SY
authors.lastname: Dai
mesh_terms: D000348:Aflatoxins / Q000032:analysis*; D000821:Animal Feed / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods*; D007553:Isotope Labeling / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000348:Aflatoxins