title: A Bayesian Markov-chain-based heteroscedastic regression model for the analysis of 18O-labeled mass spectra.
abstract: To reduce the influence of the between-spectra variability on the results of peptide quantification, one can consider the (18)O-labeling approach. Ideally, with such labeling technique, a mass shift of 4 Da of the isotopic distributions of peptides from the labeled sample is induced, which allows one to distinguish the two samples and to quantify the relative abundance of the peptides. It is worth noting, however, that the presence of small quantities of (16)O and (17)O atoms during the labeling step can cause incomplete labeling. In practice, ignoring incomplete labeling may result in the biased estimation of the relative abundance of the peptide in the compared samples. A Markov model was developed to address this issue (Zhu, Valkenborg, Burzykowski. J. Proteome Res. 9, 2669-2677, 2010). The model assumed that the peak intensities were normally distributed with heteroscedasticity using a power-of-the-mean variance funtion. Such a dependence has been observed in practice. Alternatively, we formulate the model within the Bayesian framework. This opens the possibility to further extend the model by the inclusion of random effects that can be used to capture the biological/technical variability of the peptide abundance. The operational characteristics of the model were investigated by applications to real-life mass-spectrometry data sets and a simulation study.
authors.affiliation: Department of Electrical Engineering, ESAT/SCD Katholieke Universiteit Leuven, Kasteelpark Arenberg 10, bus 2446, 3001 Heverlee, Leuven, Belgium. qi.zhu@esat.kuleuven.be
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhu
authors.forename: Tomasz
authors.initials: T
authors.lastname: Burzykowski
mesh_terms: D000818:Animals; D001499:Bayes Theorem; D002417:Cattle; D003198:Computer Simulation; D045304:Cytochromes c / Q000737:chemistry; D008390:Markov Chains; D013058:Mass Spectrometry / Q000379:methods*; D008956:Models, Chemical; D015233:Models, Statistical; D010103:Oxygen Isotopes / Q000737:chemistry*; D010446:Peptide Fragments / Q000737:chemistry*; D012044:Regression Analysis
chemical_list: D010103:Oxygen Isotopes; D010446:Peptide Fragments; D045304:Cytochromes c