title: Bionomics of two forensically important blowfly species Chrysomya megacephala and Chrysomya putoria (Diptera: Calliphoridae) reared on four types of diet.
abstract: The use of heterogeneous animal tissues for the rearing of necrophagous insect species can produce uneven biological data, which can compromise the determination of larval age and, consequently, estimates for post-mortem intervals. We investigated the development of two species, Chrysomya megacephala and Chrysomya putoria (Diptera: Calliphoridae), reared on four substrates: minced beef (control) and semi-synthetic diets with the addition of sardine, rumen or chicken eggs. No differences in total developmental times were detected among larvae reared on different diets. Length and width of larvae were partially affected by the type of food. Third instar larvae and pupae of both species were heavier on beef treatment when compared with other substrates. Overall mortality was lower when beef was used as food. Longevity of adults and sex ratio were not negatively affected by the use of diets. Egg-based diet was the least effective for both species. Given the fact that several bionomical parameters of larvae reared on diets were close to those obtained when minced beef was offered, and considering the putrid odour, frequency of contamination and lack of homogeneity of animal tissue, semi-synthetic diets can be used for rearing C. megacephala and C. putoria.
authors.affiliation: Department of Zoology, Universidade Federal de Pernambuco, Av. Prof. Moraes Rego, Recife, 50.670-420, Pernambuco, Brazil.
authors.forename: Kaynara C N
authors.initials: KC
authors.lastname: Rabêlo
authors.forename: Patrícia J
authors.initials: PJ
authors.lastname: Thyssen
authors.forename: Roberta L
authors.initials: RL
authors.lastname: Salgado
authors.forename: Manuela S C
authors.initials: MS
authors.lastname: Araújo
authors.forename: Simao D
authors.initials: SD
authors.lastname: Vasconcelos
mesh_terms: D000818:Animals; D002417:Cattle; D002645:Chickens; D004032:Diet; D004175:Diptera / Q000254:growth & development*; D004531:Eggs; D005399:Fishes; D007814:Larva / Q000254:growth & development; D008460:Meat; D011679:Pupa / Q000254:growth & development; D012417:Rumen