title: Genetic diversity and antibiotic resistance in Shigella dysenteriae and Shigella boydii strains isolated from children aged <5 years in Egypt.
abstract: Diversity within Shigella dysenteriae (n=40) and Shigella boydii (n=30) isolates from children living in Egypt aged <5 years was investigated. Shigella-associated diarrhoea occurred mainly in summer months and in children aged <3 years, it commonly presented with vomiting and fever. Serotypes 7 (30%), 2 (28%), and 3 (23%) accounted for most of S. dysenteriae isolates; 50% of S. boydii isolates were serotype 2. S. dysenteriae and S. boydii isolates were often resistant to ampicillin, chloramphenicol and tetracycline (42%, 17%, respectively), although resistance varied among serotypes. Pulsed-field gel electrophoresis separated the isolates into distinct clusters correlating with species and serotype. Genetic differences in trimethoprim/sulfamethoxazole and β-lactam-encoding resistance genes were also evident. S. dysenteriae and S. boydii are genetically diverse pathogens in Egypt; the high level of multidrug resistance associated with both pathogens and resistance to the most available inexpensive antibiotics underlines the importance of continuing surveillance.
authors.affiliation: Clinical Trials and Military Studies Program, U.S. Naval Medical Research Unit No. 3, Cairo, Egypt.
authors.forename: A M
authors.initials: AM
authors.lastname: El-Gendy
authors.forename: A
authors.initials: A
authors.lastname: Mansour
authors.forename: M A
authors.initials: MA
authors.lastname: Weiner
authors.forename: G
authors.initials: G
authors.lastname: Pimentel
authors.forename: A W
authors.initials: AW
authors.lastname: Armstrong
authors.forename: S Y N
authors.initials: SY
authors.lastname: Young
authors.forename: N
authors.initials: N
authors.lastname: Elsayed
authors.forename: J D
authors.initials: JD
authors.lastname: Klena
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002675:Child, Preschool; D024881:Drug Resistance, Bacterial; D004405:Dysentery, Bacillary / Q000453:epidemiology* / Q000382:microbiology; D004534:Egypt / Q000453:epidemiology; D016521:Electrophoresis, Gel, Pulsed-Field; D005243:Feces / Q000382:microbiology; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012761:Shigella boydii / Q000145:classification / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification*; D012762:Shigella dysenteriae / Q000145:classification / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D013420:Sulfamethoxazole / Q000494:pharmacology; D047090:beta-Lactams / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D047090:beta-Lactams; D013420:Sulfamethoxazole