title: Analysis of plasma calprotectin and polymorphisms of S100A8 in patients with aggressive periodontitis.
abstract: OBJECTIVE
  Calprotectin is an important proinflammatory mediator in various inflammatory diseases and is composed of two subunits (S100A8 and S100A9). However, the level of calprotectin in plasma of patients with aggressive periodontitis and its relationship with gene polymorphisms of S100A8 are unclear.
  
  
  METHODS
  The plasma concentrations of calprotectin were measured, using an enzyme immunoassay, in 139 patients with aggressive periodontitis and in 88 periodontally healthy control subjects. These patients were genotyped for the rs3795391 and rs3806232 polymorphisms of S100A8.
  
  
  RESULTS
  The plasma concentration of calprotectin in patients with aggressive periodontitis was significantly higher than in controls (2.17 mg/L vs. 1.72 mg/L, respectively, p = 0.001). The percentage of the AA genotype of S100A8 rs3795391 was significantly higher in patients than in controls (82% vs. 69.3%, respectively, p = 0.027), while the frequency of the allele G was decreased among patients compared with controls (9.6% vs. 16.1%, respectively, p = 0.036), which was especially apparent in men (rs3795391 genotype, p = 0.005; rs3795391 allele, p = 0.015). The mean probing depth in patients carrying the AA genotype was significantly higher than that of patients carrying the GA + GG genotype of two polymorphisms of S100A8 (rs3795391, p = 0.035; rs3806232, p = 0.040), whereas the levels of calprotectin between different genotypes were not significantly different (rs3795391, p = 0.11; rs3806232, p = 0.15).
  
  
  CONCLUSIONS
  These findings indicate that aggressive periodontitis is associated with elevated levels of plasma calprotectin and that gene polymorphisms of S100A8 may influence the susceptibility and severity of aggressive periodontitis.
authors.affiliation: Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing, China.
authors.forename: X
authors.initials: X
authors.lastname: Sun
authors.forename: H
authors.initials: H
authors.lastname: Meng
authors.forename: D
authors.initials: D
authors.lastname: Shi
authors.forename: L
authors.initials: L
authors.lastname: Xu
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: Z
authors.initials: Z
authors.lastname: Chen
authors.forename: X
authors.initials: X
authors.lastname: Feng
authors.forename: R
authors.initials: R
authors.lastname: Lu
mesh_terms: D000225:Adenine; D000328:Adult; D010520:Aggressive Periodontitis / Q000097:blood / Q000235:genetics*; D040501:Calgranulin A / Q000235:genetics*; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005838:Genotype; D006147:Guanine; D006801:Humans; D018836:Inflammation Mediators / Q000097:blood; D007958:Leukocyte Count; D039841:Leukocyte L1 Antigen Complex / Q000097:blood* / Q000235:genetics; D008297:Male; D009504:Neutrophils / Q000473:pathology; D017622:Periodontal Attachment Loss / Q000097:blood / Q000145:classification / Q000235:genetics; D010514:Periodontal Pocket / Q000097:blood / Q000145:classification / Q000235:genetics; D011110:Polymorphism, Genetic / Q000235:genetics*; D012737:Sex Factors
chemical_list: D040501:Calgranulin A; D018836:Inflammation Mediators; D039841:Leukocyte L1 Antigen Complex; D006147:Guanine; D000225:Adenine