title: Simultaneous quantification of polyherbal formulations containing Rhodiola rosea L. and Eleutherococcus senticosus Maxim. using rapid resolution liquid chromatography (RRLC).
abstract: An RRLC method capable of simultaneous identification and rapid quantification of six biologically active compounds (salidroside, tyrosol, rosarin, rosavin, rosin, rosiridin) in Rhodiola rosea L. and two active compounds (eleutheroside B and eleutheroside E) in Eleutherococcus senticosus Maxim. was developed. The chromatographic analyses were performed on a reversed phase Phenomenex C18 (2)-HST column at 40°C with a neutral mobile phase (purified water and acetonitrile) gradient system at a flow rate of 1.0ml/min and UV detection at 205 and 220nm simultaneously. Baseline separation of eight active compounds was achieved within 8min. This developed method provides good linearity (R>0.9997), precision (RSD<1.99%) and recovery of the bioactive compounds. The RRLC method developed is capable of controlling the quality of R. rosea and E. senticosus raw herbs, commercial extracts, as well as polyherbal formulations containing R. rosea and E. senticosus as ingredients. This RRLC method is accurate and sensitive; in addition, it greatly increases sample analysis throughput with reduced analysis time, which is suitable for routine quality control analysis.
authors.affiliation: Canadian Phytopharmaceuticals Corp., 12233 Riverside Way, Richmond, BC V6W 1K8, Canada.
authors.forename: Yuan-Chun
authors.initials: YC
authors.lastname: Ma
authors.forename: Xiao-Qiang
authors.initials: XQ
authors.lastname: Wang
authors.forename: Feifei
authors.initials: F
authors.lastname: Hou
authors.forename: Jie
authors.initials: J
authors.lastname: Ma
authors.forename: Mai
authors.initials: M
authors.lastname: Luo
authors.forename: Shane
authors.initials: S
authors.lastname: Lu
authors.forename: Peter
authors.initials: P
authors.lastname: Jin
authors.forename: Alice
authors.initials: A
authors.lastname: Chen
authors.forename: Iris
authors.initials: I
authors.lastname: Xu
authors.forename: Asmita V
authors.initials: AV
authors.lastname: Patel
authors.forename: Derek
authors.initials: D
authors.lastname: Gorecki
mesh_terms: D002138:Calibration; D002623:Chemistry Techniques, Analytical; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D002845:Chromatography / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods*; D004187:Disaccharides / Q000032:analysis; D028001:Eleutherococcus / Q000378:metabolism*; D005960:Glucosides / Q000032:analysis; D017705:Lignans / Q000032:analysis; D010636:Phenols / Q000032:analysis; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives / Q000032:analysis; D010666:Phenylpropionates / Q000032:analysis; D010936:Plant Extracts / Q000032:analysis* / Q000494:pharmacology; D028321:Plant Preparations / Q000032:analysis*; D011786:Quality Control; D015203:Reproducibility of Results; D012116:Resins, Plant / Q000032:analysis; D029771:Rhodiola / Q000378:metabolism*
chemical_list: D004187:Disaccharides; D005960:Glucosides; D017705:Lignans; D010636:Phenols; D010666:Phenylpropionates; D010936:Plant Extracts; D028321:Plant Preparations; D012116:Resins, Plant; C011867:4-hydroxyphenylethanol; C529148:rosavin; C013893:rosin; C421885:eleutheroside E; C028305:syringin; C009172:rhodioloside; D010626:Phenylethyl Alcohol; C529149:rosarin