title: Michler's hydrol blue: a sensitive probe for amyloid fibril detection.
abstract: Michler's hydrol blue (MHB) is investigated with respect to photophysical properties in varied solvent environment and when bound to insulin and lysozyme fibrils. The MHB chromophore is shown to act like a molecular rotor and bind well to amyloid fibrils, where it exhibits a characteristic red-shift in its excitation spectrum and an increase in the emission quantum yield upon binding. MHB is more sensitive to environmental changes than Thioflavin T (ThT) and furthermore, in contrast to the latter amyloid probe, can differentiate between insulin and lysozyme fibrils by a more red-shifted excitation spectrum for insulin fibrils. To support the experimental observations, time-dependent density functional theory (TDDFT) calculations were performed on MHB at several levels of theory. The predicted changes of spectral properties as a function of the environment are in good agreement with the experimental results. Linear dichroism (LD) is used to determine the orientation of the MHB within the fibrils. It was shown through LD and molecular modeling that MHB aligns itself preferentially parallel with the amyloid fiber at an angle of 14°-22° to the fibril axis and along the grooves of the β-sheet.
authors.affiliation: Department of Physical Chemistry, Chalmers University of Technology, Göteborg, Sweden. kitts@chalmers.se
authors.forename: Catherine C
authors.initials: CC
authors.lastname: Kitts
authors.forename: Tamás
authors.initials: T
authors.lastname: Beke-Somfai
authors.forename: Bengt
authors.initials: B
authors.lastname: Nordén
mesh_terms: D000682:Amyloid / Q000737:chemistry*; D000814:Aniline Compounds / Q000737:chemistry*; D001559:Benzhydryl Compounds / Q000737:chemistry*; D004396:Coloring Agents / Q000737:chemistry*; D007328:Insulin / Q000737:chemistry; D008958:Models, Molecular; D009113:Muramidase / Q000737:chemistry; D012996:Solutions; D013057:Spectrum Analysis / Q000379:methods; D013816:Thermodynamics
chemical_list: D000682:Amyloid; D000814:Aniline Compounds; D001559:Benzhydryl Compounds; D004396:Coloring Agents; D007328:Insulin; C558904:Michler's hydrol blue; D012996:Solutions; D009113:Muramidase