title: Improving proteome coverage on a LTQ-Orbitrap using design of experiments.
abstract: Design of experiments (DOE) was used to determine improved settings for a LTQ-Orbitrap XL to maximize proteome coverage of Saccharomyces cerevisiae. A total of nine instrument parameters were evaluated with the best values affording an increase of approximately 60% in proteome coverage. Utilizing JMP software, 2 DOE screening design tables were generated and used to specify parameter values for instrument methods. DOE 1, a fractional factorial design, required 32 methods fully resolving the investigation of six instrument parameters involving only half the time necessary for a full factorial design of the same resolution. It was advantageous to complete a full factorial design for the analysis of three additional instrument parameters. Measured with a maximum of 1% false discovery rate, protein groups, unique peptides, and spectral counts gauged instrument performance. Randomized triplicate nanoLC-LTQ-Orbitrap XL MS/MS analysis of the S. cerevisiae digest demonstrated that the following five parameters significantly influenced proteome coverage of the sample: (1) maximum ion trap ionization time; (2) monoisotopic precursor selection; (3) number of MS/MS events; (4) capillary temperature; and (5) tube lens voltage. Minimal influence on the proteome coverage was observed for the remaining four parameters (dynamic exclusion duration, resolving power, minimum count threshold to trigger a MS/MS event, and normalized collision energy). The DOE approach represents a time- and cost-effective method for empirically optimizing MS-based proteomics workflows including sample preparation, LC conditions, and multiple instrument platforms.
authors.affiliation: W. M. Keck FT-ICR Mass Spectrometry Laboratory, Department of Chemistry, North Carolina State University, Raleigh, NC 27695, USA.
authors.forename: Genna L
authors.initials: GL
authors.lastname: Andrews
authors.forename: Ralph A
authors.initials: RA
authors.lastname: Dean
authors.forename: Adam M
authors.initials: AM
authors.lastname: Hawkridge
authors.forename: David C
authors.initials: DC
authors.lastname: Muddiman
mesh_terms: D010446:Peptide Fragments; D010449:Peptide Mapping; D020543:Proteome / Q000737:chemistry; D040901:Proteomics / Q000379:methods*; D012107:Research Design; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013696:Temperature
chemical_list: D010446:Peptide Fragments; D020543:Proteome; D029701:Saccharomyces cerevisiae Proteins