title: Localization of eosinophilic esophagitis from H&E stained images using multispectral imaging.
abstract: This study is an initial investigation on the capability of multispectral imaging to capture subtle spectral information that would enable the automatic delineation between the eosinophilic esophagitis and other eosin stained tissue components, especially the RBCs. In the method, a principal component analysis (PCA) was performed on the spectral transmittance samples of the different tissue components, excluding however the transmittance samples of the eosinophilic esophagitis. From the average spectral error configuration of the eosinophilic esophagitis transmittance samples, i.e. the difference between the actual transmittance and the estimated transmittance using m PC vectors, we indentified two spectral bands by which we can localize the eosinophils. Initial results show the possibility of automatically localizing the eosinophilic esophagitis by utilizing spectral information.
authors.affiliation: Department of Pathology, Massachusetts General Hospital, Harvard Medical School Boston, MA,USA. pbautista@partners.org
authors.forename: Pinky A
authors.initials: PA
authors.lastname: Bautista
authors.forename: Yukako
authors.initials: Y
authors.lastname: Yagi
mesh_terms: D004396:Coloring Agents; D004801:Eosine Yellowish-(YS); D057765:Eosinophilic Esophagitis / Q000175:diagnosis*; D006416:Hematoxylin; D006801:Humans; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D025341:Principal Component Analysis; D013057:Spectrum Analysis / Q000379:methods*; D013194:Staining and Labeling
chemical_list: D004396:Coloring Agents; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin