title: Fluorescent core-shell star polymers based bioassays for ultrasensitive DNA detection by surface plasmon fluorescence spectroscopy.
abstract: Multilayers containing a perylene diimide labelled star polymers (FSP) donor adjacent to phosphorus dendrimer layer on a silver substrate were constructed by layer by layer (LBL) approach. Using Surface Plasmon Enhanced Fluorescence Spectroscopy (SPFS) technique, a time-resolved ultrasensitive and selective detection of DNA targets relying on enhanced optical fields associated with energy transfer (ET) were achieved under the excitation at 543 nm. The detection limit is about 8 orders of magnitude better than the achieved one under the excitation at 632 nm, which is ascribed to no energy transfer from the donor to the acceptor under the excitation at 632 nm, resulting in much weak detection signal in turn.
authors.affiliation: State Key Lab of Metal Matrix Composites, School of Materials Science & Engineering, Shanghai Jiao Tong University, 800 Dong Chuan Road, Shanghai, 200240, China. clfeng@sjtu.edu.cn
authors.forename: Chuan Liang
authors.initials: CL
authors.lastname: Feng
authors.forename: Meizhen
authors.initials: M
authors.lastname: Yin
authors.forename: Di
authors.initials: D
authors.lastname: Zhang
authors.forename: Shenmin
authors.initials: S
authors.lastname: Zhu
authors.forename: Anne Marie
authors.initials: AM
authors.lastname: Caminade
authors.forename: Jean Pierre
authors.initials: JP
authors.lastname: Majoral
authors.forename: Klaus
authors.initials: K
authors.lastname: Müllen
mesh_terms: D001681:Biological Assay / Q000295:instrumentation / Q000379:methods*; D004247:DNA / Q000032:analysis*; D005453:Fluorescence; D011108:Polymers / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods*; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods*
chemical_list: D011108:Polymers; D004247:DNA