title: Participation of the cell polarity protein PALS1 to T-cell receptor-mediated NF-κB activation.
abstract: BACKGROUND
  Beside their established function in shaping cell architecture, some cell polarity proteins were proposed to participate to lymphocyte migration, homing, scanning, as well as activation following antigen receptor stimulation. Although PALS1 is a central component of the cell polarity network, its expression and function in lymphocytes remains unknown. Here we investigated whether PALS1 is present in T cells and whether it contributes to T Cell-Receptor (TCR)-mediated activation.
  
  
  RESULTS
  By combining RT-PCR and immunoblot assays, we found that PALS1 is constitutively expressed in human T lymphocytes as well as in Jurkat T cells. siRNA-based knockdown of PALS1 hampered TCR-induced activation and optimal proliferation of lymphocyte. We further provide evidence that PALS1 depletion selectively hindered TCR-driven activation of the transcription factor NF-κB.
  
  
  CONCLUSIONS
  The cell polarity protein PALS1 is expressed in T lymphocytes and participates to the optimal activation of NF-κB following TCR stimulation.
authors.affiliation: INSERM, UMR_S 1014, Hôpital Paul Brousse, Villejuif 94800, France.
authors.forename: Gabrielle
authors.initials: G
authors.lastname: Carvalho
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Poalas
authors.forename: Catherine
authors.initials: C
authors.lastname: Demian
authors.forename: Emeline
authors.initials: E
authors.lastname: Hatchi
authors.forename: Aimé
authors.initials: A
authors.lastname: Vazquez
authors.forename: Nicolas
authors.initials: N
authors.lastname: Bidère
mesh_terms: D016764:Cell Polarity; D049109:Cell Proliferation; D006801:Humans; D019169:Jurkat Cells; D008213:Lymphocyte Activation / Q000276:immunology; D008565:Membrane Proteins / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism*; D009703:Nucleoside-Phosphate Kinase / Q000378:metabolism*; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism*; D015398:Signal Transduction / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology* / Q000378:metabolism*
chemical_list: D008565:Membrane Proteins; D016328:NF-kappa B; D011948:Receptors, Antigen, T-Cell; D009703:Nucleoside-Phosphate Kinase; C501307:MPP5 protein, human