title: Down-regulation of β-1,3-N-acetylglucosaminyltransferase-8 by siRNA inhibits the growth of human gastric cancer.
abstract: β-1,3-N-acetylglucosaminyltransferase-8 (β3Gn-T8) is the most recently identified enzyme in the β3Gn-T family, but its biological function is poorly understood. To elucidate the effects of β3Gn-T8 on gastric cancer behavior, β3Gn-T8 was down-regulated in AGS cells using small interfering RNA (siRNA). The mRNA and protein expression levels of β3Gn-T8 were detected using RT-PCR and Western blotting, respectively, and sequence-specific inhibition using siRNA was also measured using RT-PCR in human SPCA-1 and SGC-7901 cells. The cell proliferation rate was determined using MTT and the percentage of apoptotic cells was measured using flow cytometry. AGS cells transfected with β3Gn-T8 siRNA were subcutaneously transplanted into nude mice and tumorigenicity was assessed. The siRNA efficiently suppressed β3Gn-T8 expression in AGS cells, and the down-regulation of β3Gn-T8 caused significant inhibition of tumor cell growth in vitro. The apoptotic rate of AGS cells increased to 10.13% 48 h after siRNA transfection, which was five times that of the control cells. Furthermore, the knockdown of β3Gn-T8 expression reduced the tumorigenicity of gastric cancer cells in nude mice, suggesting that β3Gn-T8 has potential as a gastric cancer therapeutic target.
authors.affiliation: Department of Biochemistry and Molecular Biology, Soochow University, Suzhou, Jiangsu 215123, PR China.
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Liu
authors.forename: Li
authors.initials: L
authors.lastname: Shen
authors.forename: Lan
authors.initials: L
authors.lastname: Xu
authors.forename: Xiaoya
authors.initials: X
authors.lastname: Sun
authors.forename: Jinwei
authors.initials: J
authors.lastname: Zhou
authors.forename: Shiliang
authors.initials: S
authors.lastname: Wu
mesh_terms: D000818:Animals; D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival; D015536:Down-Regulation / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D017351:N-Acetylglucosaminyltransferases / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology*; D014162:Transfection
chemical_list: D012333:RNA, Messenger; D034741:RNA, Small Interfering; C496333:B3GNT8 protein, human; D017351:N-Acetylglucosaminyltransferases