title: Photodynamic effects of porphyrin-polyamine conjugates in human breast cancer and keratinocyte cell lines.
abstract: Porphyrin-polyamine conjugates bearing two (cis or trans position) or four spermidine or spermine units were synthesized. We studied the photostability, the hydrophilic/lipophilic balance of porphyrin-polyamine derivatives and the production of singlet oxygen. All these compounds possess physicochemical features required for their use in PDT. Then, we investigated the photocytotoxic efficacy of these porphyrin-polyamine derivatives and the cell death pathway implicated. All compounds appear to be more efficient than Photofrin® to induce HaCat and MCF7 cell death, essentially by apoptosis. Collectively, these data show that porphyrin-polyamine conjugates could be promising phototherapeutic agents.
authors.affiliation: Université de Limoges, Laboratoire de Chimie des Substances Naturelles, Faculté des Sciences et Techniques, 123 Av. Albert Thomas, 87060 Limoges, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Sarrazy
authors.forename: Guillaume
authors.initials: G
authors.lastname: Garcia
authors.forename: Jean Pierre
authors.initials: JP
authors.lastname: MBakidi
authors.forename: Caroline Le
authors.initials: CL
authors.lastname: Morvan
authors.forename: Gaëlle
authors.initials: G
authors.lastname: Bégaud-Grimaud
authors.forename: Robert
authors.initials: R
authors.lastname: Granet
authors.forename: Vincent
authors.initials: V
authors.lastname: Sol
authors.forename: Pierre
authors.initials: P
authors.lastname: Krausz
mesh_terms: D017209:Apoptosis / Q000187:drug effects* / Q000528:radiation effects; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology; D002460:Cell Line; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism; D010777:Photochemistry / Q000379:methods*; D017319:Photosensitizing Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D010789:Phototherapy / Q000379:methods; D011073:Polyamines / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D011166:Porphyrins / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D013095:Spermidine / Q000737:chemistry / Q000494:pharmacology; D013096:Spermine / Q000737:chemistry / Q000494:pharmacology; D013237:Stereoisomerism
chemical_list: D017319:Photosensitizing Agents; D011073:Polyamines; D011166:Porphyrins; D013096:Spermine; D013095:Spermidine