title: Reliability of nucleotide sequence information of full-length flagellin A gene (flaA) and flaA short variable region (SVR) for molecular discrimination of Campylobacter lari organisms.
abstract: We aimed to clarify if the thermophilic Campylobacter lari organisms including urease-negative (UN) C. lari and urease-positive thermophilic Campylobacter (UPTC) can be differentiated at the species and/or subspecies levels by employing the full-length flaA gene and flaA short variable region (SVR) nucleotide sequence information or not. Thermophilic Campylobacter isolates (n = 45) including UN C. lari (n = 17), UPTC (n = 18), and Campylobacter jejuni (n = 10) were well discriminated at the isolate level by the unweighted pair group method using arithmetic means analysis and neighbor joining procedures constructed based on the full-length flaA gene and flaA SVR nucleotide sequence information. Thus, these procedures may possibly be useful for epidemimological studies for C. lari and C. jejuni.
authors.affiliation: Laboratory of Molecular Biology, Graduate School of Environmental Health Sciences, Azabu University, Fuchinobe 1-17-71, Chuo-ku, Sagamihara, 252-5201, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Hirayama
authors.forename: A
authors.initials: A
authors.lastname: Tazumi
authors.forename: S
authors.initials: S
authors.lastname: Nakanishi
authors.forename: E
authors.initials: E
authors.lastname: Tasaki
authors.forename: K
authors.initials: K
authors.lastname: Hayashi
authors.forename: J E
authors.initials: JE
authors.lastname: Moore
authors.forename: H
authors.initials: H
authors.lastname: Ueno
authors.forename: B C
authors.initials: BC
authors.lastname: Millar
authors.forename: T
authors.initials: T
authors.lastname: Kuribayashi
authors.forename: M
authors.initials: M
authors.lastname: Matsuda
mesh_terms: D000818:Animals; D016123:Campylobacter jejuni / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D044863:Campylobacter lari / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005408:Flagellin / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D005408:Flagellin; C067138:flaA protein, bacteria