title: Effects of sinomenine on proliferation and apoptosis in human lung cancer cell line NCI-H460 in vitro.
abstract: Sinomenine (SIN) is an herbal ingredient isolated from Sinomenium acutum Rehd. et Wils. We evaluated the effects of SIN on the growth and apoptosis of human lung cancer NCI-H460 cells. SIN inhibited the proliferation of the cells in a dose- and time-dependent manner. Apoptosis was confirmed by flow cytometry, the TUNEL assay and transmission electron microscopy. SIN-induced apoptosis was accompanied by the collapse of mitochondrial membrane potential, the release of cytochrome C and the activation of caspase-9 and -3. SIN also increased Bax and decreased Bcl-2 protein levels in the NCI-H460 cells. Data analysis suggested that SIN exerted a significant apoptotic effect on NCI-H460 cells through the mitochondrial pathway. SIN is therefore a promising chemopreventive candidate for lung carcinoma.
authors.affiliation: Department of Respiratory Medicine, the Shengjing Hospital of China Medical University, Liaoning 110001, P.R. China.
authors.forename: Tingshu
authors.initials: T
authors.lastname: Jiang
authors.forename: Liping
authors.initials: L
authors.lastname: Zhou
authors.forename: Wenlu
authors.initials: W
authors.lastname: Zhang
authors.forename: Dan
authors.initials: D
authors.lastname: Qu
authors.forename: Xiaoman
authors.initials: X
authors.lastname: Xu
authors.forename: Ying
authors.initials: Y
authors.lastname: Yang
authors.forename: Shengqi
authors.initials: S
authors.lastname: Li