title: The prevalence of antimicrobial-resistant Escherichia coli in two species of invasive alien mammals in Japan.
abstract: The prevalence of antimicrobial resistance in 128 Escherichia coli isolates was investigated in two species of invasive alien mammals (IAMs): the small Asian mongoose (SAM) and Japanese weasel (JW). The SAM is found on the main island of Okinawa, Japan, where a large number of livestock is available, and the JW is present on a small island, where is isolated from the main island, and have a small number of livestock. We focused on the two IAMs, inhabiting under the different environments, and compared their prevalence of antimicrobial-resistant E. coli. In the comparison of the frequencies of antimicrobial-resistant E. coli isolates between the SAM and JW, JW showed significantly higher prevalence of resistance against three drugs, ampicillin, chlortetracycline and nalidixic acid, compared with SAM's test results (P<0.05). The bla(TEM) gene and the aph1 gene were detected in 35 subjects (91%) of ampicillin-resistant isolates and 6 subjects (100%) of kanamycin-resistant isolates, respectively. The tet (A) gene was detected in 62 subjects (46%) of CTC-resistant isolates, and the tet (B) gene was detected in 25 subjects (8%) of those in IAM. The present results suggest that some IAMs were the carrier of antimicrobial-resistant bacteria and their genes, and the frequencies of these resistances were different between two IAM species.
authors.affiliation: Department of Subtropical Agro-Production Sciences, Faculty of Agriculture, University of the Ryukyus, 1, Senbaru, Nishihara, Okinawa 903–0213, Japan.
authors.forename: Ichiro
authors.initials: I
authors.lastname: Nakamura
authors.forename: Takeshi
authors.initials: T
authors.lastname: Obi
authors.forename: Yoko
authors.initials: Y
authors.lastname: Sakemi
authors.forename: Ayano
authors.initials: A
authors.lastname: Nakayama
authors.forename: Kei
authors.initials: K
authors.lastname: Miyazaki
authors.forename: Go
authors.initials: G
authors.lastname: Ogura
authors.forename: Masanobu
authors.initials: M
authors.lastname: Tamaki
authors.forename: Tatsuzo
authors.initials: T
authors.lastname: Oka
authors.forename: Kozo
authors.initials: K
authors.lastname: Takase
authors.forename: Atsushi
authors.initials: A
authors.lastname: Miyamoto
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kawamoto
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D005243:Feces / Q000382:microbiology; D008988:Herpestidae / Q000382:microbiology*; D058865:Introduced Species; D007564:Japan; D008826:Microbial Sensitivity Tests / Q000662:veterinary; D045969:Mustelidae / Q000382:microbiology*
chemical_list: D000900:Anti-Bacterial Agents