title: Chemical grafting of a DNA intercalator probe onto functional iron oxide nanoparticles: a physicochemical study.
abstract: Spherical magnetite nanoparticles (MNPs, ∼ 24 nm in diameter) were sequentially functionalized with trimethoxysilylpropyldiethylenetriamine (TMSPDT) and a synthetic DNA intercalator, namely, 9-chloro-4H-pyrido[4,3,2-kl]acridin-4-one (PyAcr), in order to promote DNA interaction. The designed synthetic pathway allowed control of the chemical grafting efficiency to access MNPs either partially or fully functionalized with the intercalator moiety. The newly prepared nanomaterials were characterized by a range of physicochemical techniques: FTIR, TEM, PXRD, and TGA. The data were consistent with a full surface coverage by immobilized silylpropyldiethylenetriamine (SPDT) molecules, which corresponds to ∼22,300 SPDT molecules per MNP and a subsequent (4740-2940) PyAcr after the chemical grafting step (i.e., ∼ 2.4 PyAcr/nm(2)). A greater amount of PyAcr (30,600) was immobilized by the alternative strategy of binding a fully prefunctionalized shell to the MNPs with up to 16.1 PyAcr/nm(2). We found that the extent of PyAcr functionalization strongly affects the resulting properties and, particularly, the colloidal stability as well as the surface charge estimated by ζ-potential measurement. The intercalator grafting generates a negative charge contribution which counterbalances the positive charge of the single SPDT shell. The DNA binding capability was measured by titration assay and increases from 15 to 21.5 μg of DNA per mg of MNPs after PyAcr grafting (14-20% yield) but then drops to only ∼2 μg for the fully functionalized MNPs. This highlights that even if the size of the MNPs is obviously a determining factor to promote surface DNA interaction, it is not the only limiting parameter, as the mode of binding and the interfacial charge density are essential to improve loading capability.
authors.affiliation: Department of Chemistry, University of Liverpool, Liverpool, L69 7ZD, United Kingdom.
authors.forename: Laurent
authors.initials: L
authors.lastname: Bouffier
authors.forename: Humphrey H P
authors.initials: HH
authors.lastname: Yiu
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Rosseinsky
mesh_terms: D000818:Animals; D002417:Cattle; D055598:Chemical Phenomena; D004247:DNA / Q000737:chemistry*; D015342:DNA Probes / Q000737:chemistry*; D015195:Drug Design; D007364:Intercalating Agents / Q000138:chemical synthesis / Q000737:chemistry*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D010316:Particle Size
chemical_list: D015342:DNA Probes; D007364:Intercalating Agents; D058185:Magnetite Nanoparticles; D004247:DNA