title: [The regulation of UTROPHIN expression by EN1].
abstract: To investigate possible factors up-regulating the expression of UTROPHIN, potential regulatory elements in the promoter of the human UTROPHIN was predicted by P-match software and verified by EMSA and ChIP. The mechanism of EN1 regulation of the human UTROPHIN expression was evaluated by RNA interference and real-time PCR analyses. Two potential EN1 binding sites in UTROPHIN promoter region were predicted by P-Match software but only the second site was verified to interact directly with EN1 by EMSA and ChIP. The results from RNA interference and real-time PCR showed that the mRNA level of UTROPHIN increased in HeLa cells after EN1 was knockdowned by siRNA. It indicated that EN1 might be a negative regulatory factor for UTROPHIN. Our study suggested that UTROPHIN might be a new target for DMD therapy.
authors.affiliation: Higher Profession School, China Medical University, Shenyang 110001, China. wangqian529@163.com
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.forename: Dong-Hua
authors.initials: DH
authors.lastname: Cao
authors.forename: Chang-Kun
authors.initials: CK
authors.lastname: Lin
authors.forename: Zheng-Dong
authors.initials: ZD
authors.lastname: Wang
authors.forename: Wan-Ting
authors.initials: WT
authors.lastname: Cui
authors.forename: Chun-Lian
authors.initials: CL
authors.lastname: Jin
mesh_terms: D001483:Base Sequence; D005786:Gene Expression Regulation; D006367:HeLa Cells; D018398:Homeodomain Proteins / Q000502:physiology*; D006801:Humans; D008969:Molecular Sequence Data; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D049411:Utrophin / Q000235:genetics*
chemical_list: C513076:EN1 protein, human; D018398:Homeodomain Proteins; C483599:UTRN protein, human; D049411:Utrophin