title: Fluorescence turn-on detection of a protein through the displaced single-stranded DNA binding protein binding to a molecular beacon.
abstract: A new approach has been developed for the highly sensitive and selective sensing of a protein. Lysozyme binding to its aptamer prevents SSB protein binding, and the subsequent binding of the free SSB protein to a molecular beacon results in a turn-on fluorescence signal, which can be used for lysozyme quantification.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China.
authors.forename: Dan
authors.initials: D
authors.lastname: Tang
authors.forename: Dongli
authors.initials: D
authors.lastname: Liao
authors.forename: Qiankun
authors.initials: Q
authors.lastname: Zhu
authors.forename: Fangyuan
authors.initials: F
authors.lastname: Wang
authors.forename: Huping
authors.initials: H
authors.lastname: Jiao
authors.forename: Yujing
authors.initials: Y
authors.lastname: Zhang
authors.forename: Cong
authors.initials: C
authors.lastname: Yu
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods*; D002417:Cattle; D004268:DNA-Binding Proteins / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000378:metabolism*; D006801:Humans; D015345:Oligonucleotide Probes / Q000378:metabolism*; D011485:Protein Binding; D013050:Spectrometry, Fluorescence
chemical_list: D052157:Aptamers, Nucleotide; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D015345:Oligonucleotide Probes; C476430:SSB protein, E coli