title: Comparison between arterial and venous sampling of circulating hormones, substrates and peptides in severe obesity.
abstract: OBJECTIVE
  Severely obese patients are being encountered more frequently in clinical practice. Factors implicated in the relationship between obesity and cardiovascular disease may be measured from a blood sample obtained through arterial access in a cardiology setting, such as during cardiac catheterization or heart surgery. The comparability of a given sample site (arterial vs. venous) with regards to blood parameters is yet to be established.
  
  
  METHODS
  Fifteen severely obese patients undergoing bariatric surgery were recruited. Fasting blood samples were collected simultaneously from the radial artery (A) and the superior vena cava (V), both representing general circulating levels, after anesthesia but before the surgical procedure. Blood samples were analysed for glucose, insulin, non-esterified fatty acids (NEFA), leptin, adiponectin, total ghrelin, high sensitive C-reactive protein (hs-CRP) and amino-terminal pro-brain natriuretic peptide (NT-proBNP) concentrations.
  
  
  RESULTS
  Arterial and venous concentrations of all factors analysed showed no statistical difference (all p values > 0.1); leptin A: 39 ± 16 vs. V: 42 ± 18 ng/mL; total ghrelin A : 0.86 ± 0.27 vs. V : 0.76 ± 0.35 ng/mL; adiponectin A: 7.7 ± 3.3 vs. V: 7.7 ± 3.6 μg/mL; insulin A: 17.9 ± 9.7 vs. V: 18.6 ± 10.5 μU/mL; glucose A: 8.3 ± 2.1 vs. V: 7.9 ± 2.2 mM; NEFA A: 0.98 ± 0.93 vs. V: 0.89 ± 0.38 mM ; hs-CRP A: 10.17 ± 7.68 vs. V: 10.27 ± 7.30 μg/mL and NT-proBNP A: 54.3 ± 47.9 vs. V: 54.7 ± 49.3 pg/mL.
  
  
  CONCLUSIONS
  These results suggest that radial artery and superior vena cava blood collection sites are comparable and may be used clinically with respect to fasting glucose, NEFA, leptin, adiponectin, total ghrelin, hs-CRP and NT-proBNP concentrations in a group of severely obese patients.
authors.affiliation: Institut universitaire de cardiologie et de pneumologie de Québec, 2725 Chemin Ste-Foy, Québec, QC, Canada.
authors.forename: Julie
authors.initials: J
authors.lastname: Martin
authors.forename: Jessica
authors.initials: J
authors.lastname: Smith
authors.forename: Marjorie
authors.initials: M
authors.lastname: Bastien
authors.forename: Katherine
authors.initials: K
authors.lastname: Cianflone
authors.forename: Jean
authors.initials: J
authors.lastname: Bussières
authors.forename: Simon
authors.initials: S
authors.lastname: Marceau
authors.forename: Frédéric-Simon
authors.initials: FS
authors.lastname: Hould
authors.forename: Stéfane
authors.initials: S
authors.lastname: Lebel
authors.forename: Laurent
authors.initials: L
authors.lastname: Biertho
authors.forename: Odette
authors.initials: O
authors.lastname: Lescelleur
authors.forename: Simon
authors.initials: S
authors.lastname: Biron
authors.forename: Fernand
authors.initials: F
authors.lastname: Bertrand
authors.forename: Paul
authors.initials: P
authors.lastname: Poirier
mesh_terms: D000328:Adult; D005260:Female; D006728:Hormones / Q000097:blood*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000097:blood*; D010455:Peptides / Q000097:blood*
chemical_list: D006728:Hormones; D010455:Peptides