title: Cytotoxicity and apoptotic effects of nickel oxide nanoparticles in cultured HeLa cells.
abstract: The aim of this study was to observe the cytotoxicity and apoptotic effects of nickel oxide nanoparticles on human cervix epithelioid carcinoma cell line (HeLa). Nickel oxide precursors were synthesized by an nickel sulphate-excess urea reaction in boiling aqueous solution. The synthesized NiO nanoparticles (<200 nm) were investigated by X-ray diffraction analysis and transmission electron microscopy techniques. For cytotoxicity experiments, HeLa cells were incubated in 50-500 μg/mL NiO for 2, 6, 12 and 16 hours. The viable cells were counted with a haemacytometer using light microscopy. The cytotoxicity was observed low in 50-200 μg/mL concentration for 16 h, but high in 400-500 μg/mL concentration for 2-6 h. HeLa cells' cytoplasm membrane was lysed and detached from the well surface in 400 μg/mL concentration NiO nanoparticles. Double staining and M30 immunostaining were performed to quantify the number of apoptotic cells in culture on the basis of apoptotic cell nuclei scores. The apoptotic effect was observed 20% for 16 h incubation.
authors.affiliation: Department of Chemistry, Kirikkale University Faculty of Art and Sciences, Kirikkale, Turkey. kezbanada@mynet.com
authors.forename: Kezban
authors.initials: K
authors.lastname: Ada
authors.forename: Mustafa
authors.initials: M
authors.lastname: Turk
authors.forename: Serpil
authors.initials: S
authors.lastname: Oguztuzun
authors.forename: Murat
authors.initials: M
authors.lastname: Kilic
authors.forename: Mehmet
authors.initials: M
authors.lastname: Demirel
authors.forename: Nisa
authors.initials: N
authors.lastname: Tandogan
authors.forename: Ertan
authors.initials: E
authors.lastname: Ersayar
authors.forename: Ozturk
authors.initials: O
authors.lastname: Latif
mesh_terms: D017209:Apoptosis; D006367:HeLa Cells; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry / Q000633:toxicity; D046529:Microscopy, Electron, Transmission; D009532:Nickel / Q000737:chemistry / Q000633:toxicity*; D014961:X-Ray Diffraction
chemical_list: C029938:nickel sulfate; D009532:Nickel; C028007:nickel monoxide