title: Collagen hydrolysate intake increases skin collagen expression and suppresses matrix metalloproteinase 2 activity.
abstract: The effect of daily ingestion of collagen hydrolysate (CH) on skin extracellular matrix proteins was investigated. Four-week-old male Wistar rats were fed a modified AIN-93 diet containing 12% casein as the reference group or CH as the treatment group. A control group was established in which animals were fed a non-protein-modified AIN-93 diet. The diets were administered continuously for 4 weeks when six fresh skin samples from each group were assembled and subjected to extraction of protein. Type I and IV collagens were studied by immunoblot, and activities of matrix metalloproteinase (MMP) 2 and 9 were assessed by zymography. The relative amount of type I and IV collagens was significantly (P < .05) increased after CH intake compared with the reference diet group (casein). Moreover, CH uptake significantly decreased both proenzyme and active forms of MMP2 compared with casein and control groups (P < .05). In contrast, CH ingestion did not influence on MMP9 activity. These results suggest that CH may reduce aging-related changes of the extracellular matrix by stimulating anabolic processes in skin tissue.
authors.affiliation: Department of Cell and Developmental Biology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil. vizague@hotmail.com
authors.forename: Vivian
authors.initials: V
authors.lastname: Zague
authors.forename: Vanessa
authors.initials: V
authors.lastname: de Freitas
authors.forename: Marina
authors.initials: M
authors.lastname: da Costa Rosa
authors.forename: Geórgia Álvares
authors.initials: GÁ
authors.lastname: de Castro
authors.forename: Ruy G
authors.initials: RG
authors.lastname: Jaeger
authors.forename: Gláucia M
authors.initials: GM
authors.lastname: Machado-Santelli
mesh_terms: D000818:Animals; D003094:Collagen / Q000008:administration & dosage* / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D019587:Dietary Supplements / Q000032:analysis; D015536:Down-Regulation / Q000187:drug effects*; D006801:Humans; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism*; D011492:Protein Hydrolysates / Q000008:administration & dosage* / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism / Q000503:physiopathology; D015595:Skin Aging / Q000187:drug effects* / Q000235:genetics
chemical_list: D011492:Protein Hydrolysates; D003094:Collagen; D020778:Matrix Metalloproteinase 2