title: Catellibacterium nanjingense sp. nov., a propanil-degrading bacterium isolated from activated sludge, and emended description of the genus Catellibacterium.
abstract: A novel facultatively anaerobic, non-spore-forming, non-motile, catalase- and oxidase-positive, Gram-negative and rod-shaped bacterial strain, designated Y12(T), was isolated from activated sludge of a wastewater bio-treatment facility. The strain was able to degrade about 90% of added propanil (100 mg l(-1)) within 3 days of incubation. Growth occurred in the presence of 0-4.5% (w/v) NaCl (optimum 0.5%), at 10-40 °C (optimum 28 °C) and at pH 5.5-10.0 (optimum pH 7.0). Vesicular internal membrane structures and photoheterotrophic growth were not observed. The major respiratory quinone was ubiquinone-10 and the major cellular fatty acid was summed feature 8 (C(18:1)ω6c and/or C(18:1)ω7c). The genomic DNA G+C content of strain Y12(T) was 63.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that strain Y12(T) was a member of the genus Catellibacterium, as it showed highest sequence similarities to Catellibacterium caeni DCA-1(T) (99.1%) and <96.0% similarities with other species of the genus Catellibacterium. Strain Y12(T) showed low DNA-DNA relatedness values with C. caeni DCA-1(T). Based on phenotypic, genotypic and phylogenetic properties, strain Y12(T) represents a novel species of the genus Catellibacterium, for which the name Catellibacterium nanjingense sp. nov. is proposed. The type strain is Y12(T) (=CCTCC AB 2010218(T) =KCTC 23298(T)). An emended description of the genus Catellibacterium is also presented.
authors.affiliation: Key Laboratory for Microbiological Engineering of Agricultural Environment of Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: China National Center for Biotechnology Development, Beijing 210095, PR China.
authors.forename: Shu-An
authors.initials: SA
authors.lastname: Chen
authors.affiliation: Key Laboratory for Microbiological Engineering of Agricultural Environment of Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Jin-Wei
authors.initials: JW
authors.lastname: Zheng
authors.affiliation: Key Laboratory for Microbiological Engineering of Agricultural Environment of Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Shu
authors.initials: S
authors.lastname: Cai
authors.affiliation: Key Laboratory for Microbiological Engineering of Agricultural Environment of Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Bao-Jian
authors.initials: BJ
authors.lastname: Hang
authors.affiliation: Key Laboratory for Microbiological Engineering of Agricultural Environment of Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: He
authors.affiliation: Key Laboratory for Microbiological Engineering of Agricultural Environment of Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Shun-Peng
authors.initials: SP
authors.lastname: Li
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D011411:Propanil / Q000378:metabolism*; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D041902:Rhodobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D012965:Sodium Chloride; D011411:Propanil