title: Rapid detection of enterotoxigenic Escherichia coli gene using bio-conjugated gold nano-particles.
abstract: In this study, a simple gold nanoparticle based colorimetric detection system for on site diagnostics of Enterotoxigenic Escherichia coli (ETEC) has been developed. The developed assay could detect LT1 gene as evident from UV-visible spectra, the broadening of peak on hybridization of GNP probes with different copy numbers of the target gene. The present study suggests that hybridization induced changes in optical properties of GNPs can be translated into a rapid and simple colorometric" spot and read test detection method for ETEC.
authors.affiliation: Environmental Toxicology Group, Indian Institute of Toxicology Research (CSIR), P.O. Box No. 80, Mahatma Gandhi Marg, Lucknow 226001, India.
authors.forename: Anurag
authors.initials: A
authors.lastname: Jyotil
authors.forename: Surinder P
authors.initials: SP
authors.lastname: Singh
authors.forename: Madhu
authors.initials: M
authors.lastname: Yashpal
authors.forename: Premendra D
authors.initials: PD
authors.lastname: Dwivedi
authors.forename: Rishi
authors.initials: R
authors.lastname: Shanker
mesh_terms: D001427:Bacterial Toxins / Q000032:analysis*; D015374:Biosensing Techniques / Q000379:methods*; D054307:Enterotoxigenic Escherichia coli / Q000302:isolation & purification* / Q000378:metabolism; D004768:Enterotoxins / Q000032:analysis*; D029968:Escherichia coli Proteins / Q000032:analysis*; D020869:Gene Expression Profiling / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D006046:Gold; C027538:heat-labile enterotoxin, E coli