title: Prevalence of Helicobacter pylori genotypes (vacA, cagA, cagE and virB11) in gastric cancer in Brazilian's patients: an association with histopathological parameters.
abstract: OBJECTIVE
  To investigate the frequency and the association of vacA alleles, cagA, cagE and virB11 genes of Helicobacter pylori from patients with gastric cancer, considering the clinic histopathological parameters.
  
  
  METHODS
  One hundred and one gastric adenocarcinoma tissues were assessed by PCR to detect H. pylori and vacA alleles, cagA, cagE and virB11.
  
  
  RESULTS
  The distribution of cases according to the presence of the genes studied showed that the group containing vacA s1m1, cagA, cagE and virB11 H. pylori genes was significantly more frequent, followed by the group with at least one marker on the right side and left of the island. They were also present in the early stages and were the most frequent in nearly all histopathological grades.
  
  
  CONCLUSIONS
  This study verified that vacAs1m1 and cag-PAI genes, cagA, cagE and virB11 are important H. pylori markers for gastric cancer development. Also, this study corroborates the importance of cagE and cagA together as cag-PAI marker.
authors.affiliation: Laboratório de Genética Molecular-LABGEM, Departamento de Patologia e Medicina Legal, Universidade Federal do Ceará-UFC, Brazil. valeskacb@yahoo.com.br
authors.forename: Valeska Portela
authors.initials: VP
authors.lastname: Lima
authors.forename: Isabelle Joyce de Lima
authors.initials: IJ
authors.lastname: Silva-Fernandes
authors.forename: Markênia Kélia Santos
authors.initials: MK
authors.lastname: Alves
authors.forename: Silvia Helena Barem
authors.initials: SH
authors.lastname: Rabenhorst
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology / Q000821:virology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000942:Antigens, Bacterial / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D001938:Brazil / Q000453:epidemiology; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D005500:Follow-Up Studies; D016481:Helicobacter Infections / Q000235:genetics / Q000473:pathology / Q000821:virology; D016480:Helicobacter pylori / Q000235:genetics* / Q000472:pathogenicity; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction; D015995:Prevalence; D011379:Prognosis; D013274:Stomach Neoplasms / Q000453:epidemiology / Q000235:genetics / Q000473:pathology* / Q000821:virology*; D037521:Virulence Factors / Q000235:genetics*; D055815:Young Adult
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D004279:DNA, Viral; C103937:PicB protein, Helicobacter pylori; C074689:VacA protein, Helicobacter pylori; D037521:Virulence Factors; C080866:cagA protein, Helicobacter pylori