title: Analysis of indole compounds in Armillaria mellea fruiting bodies.
abstract: A TLC method with densitometric detection for identification and quantitation of indole compounds in methanolic extracts of fruiting bodies of Armillaria mellea (Vahl.) P. Kumm. (Basidiomycota)--Honey mushroom has been developed. The TLC method with densitometric detection was validated for determination of tryptamine and serotonin contents. The use of this chromatographic-densitometric method for analysis of extracts allowed for a precise, easy and quick determination of the compounds under study. We identified three physiologically active indole compounds: tryptamine. L- tryptophan and serotonin. This analysis demonstrated the highest contents of L-tryptophan (4.467 mg/100 g d.w.). The contents of tryptamine were comparable with the contents of serotonin and amounted to: 2.740 and 2.207 mg/100 g d. w.
authors.affiliation: Chair and Department of Pharmaceutical Botany, Jagiellonian University, Collegium Medicum, Medyczna 9, 30-688 Kraków, Poland. muchon@poczta.fm
authors.forename: Bozena
authors.initials: B
authors.lastname: Muszyńska
authors.forename: Anna
authors.initials: A
authors.lastname: Maślanka
authors.forename: Halina
authors.initials: H
authors.lastname: Ekiert
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Sułkowska-Ziaja
mesh_terms: D055392:Armillaria / Q000737:chemistry*; D002855:Chromatography, Thin Layer; D003720:Densitometry; D048690:Fruiting Bodies, Fungal / Q000737:chemistry*; D007211:Indoles / Q000032:analysis*; D000432:Methanol / Q000737:chemistry; D015203:Reproducibility of Results; D012701:Serotonin / Q000032:analysis; D012997:Solvents / Q000737:chemistry; D014363:Tryptamines / Q000032:analysis; D014364:Tryptophan
chemical_list: D007211:Indoles; D012997:Solvents; D014363:Tryptamines; D012701:Serotonin; C030820:tryptamine; D014364:Tryptophan; D000432:Methanol