title: Antimicrobial activity of [2-(methacryloyloxy)ethyl]trimethylammonium chloride against Candida spp.
abstract: BACKGROUND
  Candida-associated denture stomatitis is the most common manifestation of oral candidal infection, caused mainly by Candida albicans. Several authors have attempted to add antifungal agents or antiseptics to denture temporary soft lining materials or to denture acrylic resins, without relevant results. Therefore, the investigation of a quaternary ammonium functionalized compound [2-(methacryloyloxy)ethyl]trimethylammonium chloride (MADQUAT), which copolymerizes with methacrylates and which could act as a fungal inhibitor, is of paramount importance.
  
  
  OBJECTIVE
  To evaluate the in vitro activity of MADQUAT against Candida species.
  
  
  METHODS
  Thirty-one Candida strains were used to determine the in vitro antifungal activity of this compound. The minimum inhibitory concentrations and minimum fungicidal concentrations of MADQUAT and nystatin were determined.
  
  
  RESULTS
  MADQUAT showed antifungal properties at concentrations of 6.25 to > 100mg/ml, and fungicidal activity between 25 and > 100mg/ml. The quantitative determinations of the fungistatic and fungicidal activity of MADQUAT showed fungistatic activity against all Candida albicans, Candida krusei and Candida parapsilosis strains, revealing fungicidal activity against some strains of the other species.
  
  
  CONCLUSIONS
  MADQUAT has antifungal activity against Candida spp. Moreover, the sensitivity to this substance varies across the different species in terms of MIC values and fungicidal or fungistatic activity.
authors.affiliation: Graduate Program in Medicine, Medical Sciences, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.
authors.forename: Cheila Denise Ottonelli
authors.initials: CD
authors.lastname: Stopiglia
authors.forename: Fabrício Mezzomo
authors.initials: FM
authors.lastname: Collares
authors.forename: Fabrício Aulo
authors.initials: FA
authors.lastname: Ogliari
authors.forename: Evandro
authors.initials: E
authors.lastname: Piva
authors.forename: Carmen Beatriz Borges
authors.initials: CB
authors.lastname: Fortes
authors.forename: Susana Maria Werner
authors.initials: SM
authors.lastname: Samuel
authors.forename: Maria Lúcia
authors.initials: ML
authors.lastname: Scroferneker
mesh_terms: D002175:Candida / Q000187:drug effects*; D008689:Methacrylates / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests
chemical_list: C120889:2-(trimethylammonio)ethyl methacrylate; D008689:Methacrylates