title: Biomedical soft contact-lens sensor for in situ ocular biomonitoring of tear contents.
abstract: A soft contact-lens biosensor (SCL-biosensor) for novel non-invasive biomonitoring of tear fluids was fabricated and tested. Wearing a biosensor on eye enabled the in situ monitoring of tear contents. The biosensor has an enzyme immobilized electrode on the surface of a polydimethyl siloxane (PDMS) contact lens. The SCL-biosensor was fabricated using microfabrication techniques for functional polymers (PDMS and 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer). In investigation of in vitro characterization, the SCL-biosensor showed excellent relationship between the output current and glucose concentration from 0.03 to 5.0 mmol·L(-1), with a correlation coefficient of 0.994. The calibration range covered the reported tear glucose concentrations (0.14 mmol·L(-1)). Based on the result, ocular biomonitoring with the SCL-biosensor was carried out. The SCL-biosensor well worked both in the static state and the dynamic state. The tear glucose level of rabbit was estimated to 0.12 mmol·L(-1) at first and then the tear turnover was successfully calculated to be 29.6 ± 8.42% min(-1). The result indicated that SCL-biosensor is useful for advanced biomonitoring on eye.
authors.affiliation: Department of Advanced Science and Technology for Biomedical Sensors, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan.
authors.forename: MingXing
authors.initials: M
authors.lastname: Chu
authors.forename: Takayuki
authors.initials: T
authors.lastname: Shirai
authors.forename: Daishi
authors.initials: D
authors.lastname: Takahashi
authors.forename: Takahiro
authors.initials: T
authors.lastname: Arakawa
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kudo
authors.forename: Kenji
authors.initials: K
authors.lastname: Sano
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Sawada
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Yano
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Iwasaki
authors.forename: Kazunari
authors.initials: K
authors.lastname: Akiyoshi
authors.forename: Manabu
authors.initials: M
authors.lastname: Mochizuki
authors.forename: Kohji
authors.initials: K
authors.lastname: Mitsubayashi
mesh_terms: D000818:Animals; D020811:Biomedical Technology / Q000295:instrumentation*; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D002138:Calibration; D003263:Contact Lenses, Hydrophilic; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D005123:Eye / Q000378:metabolism; D005947:Glucose / Q000032:analysis; D008689:Methacrylates / Q000737:chemistry; D055616:Microtechnology / Q000379:methods; D010767:Phosphorylcholine / Q000031:analogs & derivatives / Q000737:chemistry; D011817:Rabbits; D013666:Tears / Q000737:chemistry*; D014785:Vision, Ocular
chemical_list: D004129:Dimethylpolysiloxanes; D008689:Methacrylates; D010767:Phosphorylcholine; C070638:2-methacryloyloxyethyl phosphorylcholine; C013830:baysilon; D005947:Glucose