title: Silicon biochips for dual label-free and fluorescence detection: Application to protein microarray development.
abstract: A new silicon chip for protein microarray development, fabrication and validation is proposed. The chip is made of two areas with oxide layers of different thicknesses: an area with a 500 nm SiO2 layer dedicated to interferometric label-free detection and quantification of proteins and an area with 100 nm SiO2 providing enhanced fluorescence. The chip allows, within a single experiment performed on the same surface, label-free imaging of arrayed protein probes coupled with high sensitivity fluorescence detection of the molecular interaction counterparts. Such a combined chip is of high practical utility during assay development process to image arrays, check consistency and quality of the protein array, quantify the amount of immobilized probes and finally detect fluorescence of bioassays.
authors.affiliation: Consiglio Nazionale delle Ricerche, Istituto di Chimica del Riconoscimento Molecolare (ICRM), Milano, Italy.
authors.forename: Marina
authors.initials: M
authors.lastname: Cretich
authors.affiliation: Department of Electrical and Computer Engineering, Boston University, 8 St. Mary's St., Boston, MA, United States.
authors.forename: Alex
authors.initials: A
authors.lastname: Reddington
authors.affiliation: Department of Electrical and Computer Engineering, Boston University, 8 St. Mary's St., Boston, MA, United States.
authors.forename: Margo
authors.initials: M
authors.lastname: Monroe
authors.affiliation: Consiglio Nazionale delle Ricerche, Istituto di Chimica del Riconoscimento Molecolare (ICRM), Milano, Italy.
authors.forename: Marta
authors.initials: M
authors.lastname: Bagnati
authors.affiliation: Consiglio Nazionale delle Ricerche, Istituto di Chimica del Riconoscimento Molecolare (ICRM), Milano, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Damin
authors.affiliation: Consiglio Nazionale delle Ricerche, Istituto di Chimica del Riconoscimento Molecolare (ICRM), Milano, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Sola
authors.affiliation: Department of Electrical and Computer Engineering, Boston University, 8 St. Mary's St., Boston, MA, United States; Department of Biomedical Engineering, Boston University, 44 Cummington St., Boston, MA, United States; Physics Department, Boston University, 590 Commonwealth Ave., Boston, MA, United States.
authors.forename: M Selim
authors.initials: MS
authors.lastname: Unlu
authors.affiliation: Consiglio Nazionale delle Ricerche, Istituto di Chimica del Riconoscimento Molecolare (ICRM), Milano, Italy.
authors.forename: Marcella
authors.initials: M
authors.lastname: Chiari
mesh_terms: D015374:Biosensing Techniques; D005453:Fluorescence; D011108:Polymers / Q000737:chemistry; D040081:Protein Array Analysis / Q000379:methods*; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*; D012822:Silicon Dioxide / Q000737:chemistry; D020349:Surface Plasmon Resonance
chemical_list: D011108:Polymers; D011506:Proteins; D012822:Silicon Dioxide