title: Relationship between peroxisome proliferator-activated receptor-γ and renal ischemia-reperfusion injury.
abstract: The pathogenesis of ischemia-reperfusion (I/R) injury is known to involve cytokines and, in particular, surface adhesion molecules, the expression of which initiates inflammatory cell attachment. It has been suggested that peroxisome proliferator-activated receptor (PPAR)-γ is an important immunomodulatory factor as well as a regulator of fatty acid. In this study, we investigated the expression of PPAR-γ in a renal I/R injury rat model. The right kidney was harvested and the left renal artery and vein were clamped by means of a laparotomy. The kidney was reperfused following 90 min of ischemia. Rats were sacrificed at 0, 1.5, 3, 5, 12 and 24 h after reperfusion. PPAR-γ expression was analyzed by immunohistochemical staining using monoclonal antibody. PPAR-γ staining was weak in the endothelial cells, interstitial cells and collecting ducts in the normal kidney. From 1.5 to 5 h after reperfusion, PPAR-γ staining was strong. Twelve hours after reperfusion, necrosis had extended throughout the kidney, and nearly all the tubular epithelial cells were destroyed. However, 12 h after reperfusion, PPAR-γ staining was weak in the endothelial cells and its expression was moderate in the interstitial cells and collecting ducts. PPAR-γ was induced in the endothelial cells, including the mesangial cells, interstitial cells and collecting ducts in a rat model of renal I/R injury.
authors.affiliation: Department of Urology, Osaka City University Graduate School of Medicine, Osaka 545-8585, Japan.
authors.forename: Masahide
authors.initials: M
authors.lastname: Matsuyama
authors.forename: Rikio
authors.initials: R
authors.lastname: Yoshimura
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Kawahito
authors.forename: Hajime
authors.initials: H
authors.lastname: Sano
authors.forename: Jamel
authors.initials: J
authors.lastname: Chargui
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Touraine
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Nakatani