title: Immunocytochemical demonstration of M(1) muscarinic acetylcholine receptors at the presynaptic and postsynaptic membranes of rat diaphragm endplates.
abstract: M(1)-muscarinic acetylcholine (ACh) receptors (M(1)R) were directly demonstrated immunocytochemically in electronmicroscopic images of rat diaphragm neuromuscular junctions (NMJ). Specific electron-dense granules were located at presynaptic nerve ending membranes and in the sarcolemma in the depths of postsynaptic folds. This first visualization of M(1)R on both sides of the NMJ is in agreement with previous pharmacological data on the regulatory role of M(1)R in quantal and non-quantal ACh release.
authors.affiliation: Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: A I
authors.initials: AI
authors.lastname: Malomouzh
authors.forename: S S
authors.initials: SS
authors.lastname: Arkhipova
authors.forename: E E
authors.initials: EE
authors.lastname: Nikolsky
authors.forename: F
authors.initials: F
authors.lastname: Vyskočil
mesh_terms: D000818:Animals; D003964:Diaphragm / Q000294:innervation; D007150:Immunohistochemistry; D009045:Motor Endplate / Q000378:metabolism* / Q000648:ultrastructure; D009469:Neuromuscular Junction / Q000378:metabolism; D017729:Presynaptic Terminals / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats; D011976:Receptors, Muscarinic / Q000378:metabolism*; D013570:Synaptic Membranes / Q000378:metabolism*; D009435:Synaptic Transmission / Q000502:physiology*
chemical_list: D011976:Receptors, Muscarinic