title: microRNA-214 contributes to melanoma tumour progression through suppression of TFAP2C.
abstract: Malignant melanoma is fatal in its metastatic stage. It is therefore essential to unravel the molecular mechanisms that govern disease progression to metastasis. MicroRNAs (miRs) are endogenous non-coding RNAs involved in tumourigenesis. Using a melanoma progression model, we identified a novel pathway controlled by miR-214 that coordinates metastatic capability. Pathway components include TFAP2C, homologue of a well-established melanoma tumour suppressor, the adhesion receptor ITGA3 and multiple surface molecules. Modulation of miR-214 influences in vitro tumour cell movement and survival to anoikis as well as extravasation from blood vessels and lung metastasis formation in vivo. Considering that miR-214 is known to be highly expressed in human melanomas, our data suggest a critical role for this miRNA in disease progression and the establishment of distant metastases.
authors.affiliation: Molecular Biotechnology Center (MBC), University of Torino, Torino, Italy.
authors.forename: Elisa
authors.initials: E
authors.lastname: Penna
authors.forename: Francesca
authors.initials: F
authors.lastname: Orso
authors.forename: Daniela
authors.initials: D
authors.lastname: Cimino
authors.forename: Enrico
authors.initials: E
authors.lastname: Tenaglia
authors.forename: Antonio
authors.initials: A
authors.lastname: Lembo
authors.forename: Elena
authors.initials: E
authors.lastname: Quaglino
authors.forename: Laura
authors.initials: L
authors.lastname: Poliseno
authors.forename: Adele
authors.initials: A
authors.lastname: Haimovic
authors.forename: Simona
authors.initials: S
authors.lastname: Osella-Abate
authors.forename: Cristiano
authors.initials: C
authors.lastname: De Pittà
authors.forename: Eva
authors.initials: E
authors.lastname: Pinatel
authors.forename: Michael B
authors.initials: MB
authors.lastname: Stadler
authors.forename: Paolo
authors.initials: P
authors.lastname: Provero
authors.forename: Maria Grazia
authors.initials: MG
authors.lastname: Bernengo
authors.forename: Iman
authors.initials: I
authors.lastname: Osman
authors.forename: Daniela
authors.initials: D
authors.lastname: Taverna
mesh_terms: D000818:Animals; D002465:Cell Movement; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D006801:Humans; D016023:Integrins / Q000378:metabolism; D008168:Lung / Q000473:pathology; D008175:Lung Neoplasms / Q000473:pathology; D008545:Melanoma / Q000473:pathology* / Q000556:secondary*; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009362:Neoplasm Metastasis / Q000473:pathology*; D050656:Transcription Factor AP-2 / Q000096:biosynthesis*
chemical_list: D016023:Integrins; C547397:MIRN214 microRNA, human; D035683:MicroRNAs; C493679:TFAP2C protein, human; D050656:Transcription Factor AP-2