title: Detection of vitellogenin incorporation into zebrafish oocytes by FITC fluorescence.
abstract: BACKGROUND
  Large volumes of lymph can be collected from the eye-sacs of bubble-eye goldfish. We attempted to induce vitellogenin (Vtg) in the eye-sac lymph of bubble-eye goldfish and develop a method for visualizing Vtg incorporation by zebrafish oocytes using FITC-labeling.
  
  
  METHODS
  Estrogen efficiently induced Vtg in the eye-sac lymph of goldfish. After FITC-labeled Vtg was prepared, it was injected into mature female zebrafish.
  
  
  RESULTS
  Incorporation of FITC-labeled Vtg by zebrafish oocytes was detected in in vivo and in vitro experiments. The embryos obtained from zebrafish females injected with FITC-labeled Vtg emitted FITC fluorescence from the yolk sac and developed normally.
  
  
  CONCLUSIONS
  This method for achieving Vtg incorporation by zebrafish oocytes could be useful in experiments related to the development and endocrinology of zebrafish oocytes.
authors.affiliation: Laboratory of Marine Life Science and Genetics, Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai 981-8555, Japan.
authors.forename: Yohei
authors.initials: Y
authors.lastname: Matsuda
authors.forename: Yoshie
authors.initials: Y
authors.lastname: Ito
authors.forename: Hisashi
authors.initials: H
authors.lastname: Hashimoto
authors.forename: Hayato
authors.initials: H
authors.lastname: Yokoi
authors.forename: Tohru
authors.initials: T
authors.lastname: Suzuki
mesh_terms: D000818:Animals; D004967:Estrogens / Q000494:pharmacology; D005260:Female; D016650:Fluorescein-5-isothiocyanate / Q000032:analysis; D006054:Goldfish / Q000378:metabolism*; D008196:Lymph / Q000378:metabolism; D008856:Microscopy, Fluorescence / Q000379:methods*; D014819:Vitellogenins / Q000032:analysis / Q000378:metabolism*; D015027:Zebrafish / Q000378:metabolism*
chemical_list: D004967:Estrogens; D014819:Vitellogenins; D016650:Fluorescein-5-isothiocyanate