title: Gecko CD59 is implicated in proximodistal identity during tail regeneration.
abstract: Several adult reptiles, such as Gekko japonicus, have the ability to precisely re-create a missing tail after amputation. To ascertain the associated acquisition of positional information from blastemal cells and the underlying molecular mechanism of tail regeneration, a candidate molecule CD59 was isolated from gecko. CD59 transcripts displayed a graded expression in the adult gecko spinal cord with the highest level in the anterior segment, with a stable expression along the normal tail. After tail amputation, CD59 transcripts in the spinal cord proximal to the injury sites increased markedly at 1 day and 2 weeks; whereas in the regenerating blastema, strong CD59 positive signals were detected in the blastemal cells anterior to the blastema, with a gradual decrease along the proximodistal (PD) axis. When treated with RA following amputation, CD59 transcripts in the blastema were up-regulated. PD confrontation assays revealed that the proximal blastema engulfed the distal one after in vitro culture, and rabbit-anti human CD59 antibody was able to block this PD engulfment. Overexpression of the CD59 during tail regeneration causes distal blastemal cells to translocate to a more proximal location. Our results suggest that position identity is not restricted to amphibian limb regeneration, but has already been established in tail blastema of reptiles. The CD59, a cell surface molecule, acted as a determinant of proximal-distal cell identity.
authors.affiliation: Key Laboratory of Neuroregeneration, Nantong University, Nantong, China.
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Wang
authors.forename: Ruili
authors.initials: R
authors.lastname: Wang
authors.forename: Shengjuan
authors.initials: S
authors.lastname: Jiang
authors.forename: Weijuan
authors.initials: W
authors.lastname: Zhou
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Wang
authors.forename: Qing
authors.initials: Q
authors.lastname: Gu
authors.forename: Yun
authors.initials: Y
authors.lastname: Gu
authors.forename: Yingying
authors.initials: Y
authors.lastname: Dong
authors.forename: Mei
authors.initials: M
authors.lastname: Liu
authors.forename: Xingxing
authors.initials: X
authors.lastname: Gu
authors.forename: Fei
authors.initials: F
authors.lastname: Ding
authors.forename: Xiaosong
authors.initials: X
authors.lastname: Gu
mesh_terms: D000595:Amino Acid Sequence; D000671:Amputation, Surgical; D000818:Animals; D018957:CD59 Antigens / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D020869:Gene Expression Profiling; D006801:Humans; D008116:Lizards / Q000378:metabolism*; D008969:Molecular Sequence Data; D021381:Protein Transport / Q000187:drug effects; D012038:Regeneration / Q000187:drug effects / Q000502:physiology*; D016415:Sequence Alignment; D013116:Spinal Cord / Q000187:drug effects / Q000378:metabolism; D013623:Tail / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D014162:Transfection; D014212:Tretinoin / Q000494:pharmacology; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D018957:CD59 Antigens; D014212:Tretinoin