title: [Study on concentration of nuorovirus genegroup II from environmental water].
abstract: A new viral sampling concentration device was designed which was equipped with a new cationic filter membrane-Nanoceram suitable for field sampling. Norovirus Genegroup II was detected from environmental water with the aid of this device. The effects on virus recovery of prefiltration, various second-concentration methods, and different eluants were investigated through pre-experiment. The concentration optimized process, and the optimal concentration process were then determined. The results showed that the prefiltration had a profound effect on virus recovery, and two second-concentration method: PEG-NaC1 precipitation and celite adsorption, had almost the same concentration effects. The Na2 HPO4 solution of 0.15 mol/L was selected as the final eluant to elute the adsorbed Nuorovirus from the celite. The virus recovery of Nanoceram was determined to be 3.02%. Finally, successful detection of Norovirus GII in sewage from Yangqiao River, Fengtai District, Beijing was acheived. All these data had shown that the Naneceram filter concentration method could concentrate Norovirus from environmental water with a steady effects.
authors.affiliation: Nanhua University, College of Public Health, Hengyang 421001, China.
authors.forename: Sha
authors.initials: S
authors.lastname: Liang
authors.forename: Guang-cheng
authors.initials: GC
authors.lastname: Xie
authors.forename: Zi-qian
authors.initials: ZQ
authors.lastname: Xu
authors.forename: Jin-song
authors.initials: JS
authors.lastname: Li
authors.forename: Dan-tong
authors.initials: DT
authors.lastname: Li
authors.forename: Shao-long
authors.initials: SL
authors.lastname: Feng
authors.forename: Zhao-jun
authors.initials: ZJ
authors.lastname: Duan
mesh_terms: D005374:Filtration / Q000295:instrumentation / Q000379:methods*; D005590:Fractional Precipitation / Q000295:instrumentation / Q000379:methods*; D005838:Genotype; D029322:Norovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D045483:Rivers / Q000821:virology; D014871:Water Microbiology