title: Chicken adipocyte fatty acid-binding protein knockdown affects expression of peroxisome proliferator-activated receptor γ gene during oleate-induced adipocyte differentiation.
abstract: Adipocyte fatty acid-binding protein (A-FABP) is expressed in both adipose cells and macrophages. As one of the downstream genes of peroxisome proliferator-activated receptor γ (PPAR-γ), A-FABP plays an important role in the lipid metabolism of adipocytes in mammal and poultry. However, studies in A-FABP null macrophages of mice showed that A-FABP was a critical regulator of PPAR-γ and could affect the expression of PPAR-γ. The current study was designed to investigate whether the same mechanism as that in macrophages exists in chicken adipocytes. After transfection with interference and overexpression plasmids of A-FABP in chicken adipocytes for 24 h, oleate was added to the medium. Then, lipid accumulation, nonesterified fatty acids (NEFA) in the medium, and expression of lipid metabolism-related genes were detected. The results showed that in the A-FABP knockdown adipocytes, lipid accumulation was decreased at 6 h and NEFA in the medium was higher at 1 and 6 h compared with that in the control group. Moreover, gene expression levels of lipoprotein lipase, perilipin, and PPAR-γ were higher than that of the control group (P < 0.05). In the A-FABP overexpression adipocytes, lipid accumulation and expression of lipid metabolism related genes were similar to that of the control group. However, NEFA in the medium was significantly lower in the A-FABP overexpression group 1 h after adding oleate (P < 0.05). The present study suggested that the A-FABP knockdown might lead to decreased lipid accumulation and upregulated expression of PPAR-γ in chicken adipocytes.
authors.affiliation: College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, P. R. China.
authors.forename: H
authors.initials: H
authors.lastname: Shi
authors.forename: Q
authors.initials: Q
authors.lastname: Zhang
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: P
authors.initials: P
authors.lastname: Yang
authors.forename: Q
authors.initials: Q
authors.lastname: Wang
authors.forename: H
authors.initials: H
authors.lastname: Li
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D002645:Chickens / Q000378:metabolism*; D050556:Fatty Acid-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D005786:Gene Expression Regulation / Q000502:physiology*; D020868:Gene Silencing; D050356:Lipid Metabolism; D008297:Male; D051379:Mice; D019301:Oleic Acid / Q000494:pharmacology*; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism*
chemical_list: D050556:Fatty Acid-Binding Proteins; D005230:Fatty Acids, Nonesterified; D047495:PPAR gamma; D019301:Oleic Acid