title: Simultaneous determination of catecholamines and their metabolites related to Alzheimer's disease in human urine.
abstract: A simple and specific high-performance liquid chromatography method coupled with fluorescence detection (HPLC-FL) has been developed for the simultaneous determination of L-3,4-dihydroxyphenylalanine, norepinephrine, dopamine, epinephrine and 3,4-dihydroxyphenylacetic acid in human urine. The samples were derivatized by 1,2-diphenylethylenediamine with isoprenaline as internal standard. The factors affecting the fluorescence yield were investigated, including the reaction and separation conditions. The catecholamine derivatives were separated on a Kromasil C(18) column with methanol and sodium acetate buffer as mobile phase. The limits of detection for all catecholamines ranged from 0.2 to 1.1 ng/mL. The linear ranges were from 2.5 to 200 ng/mL except 3,4-dihydroxyphenylacetic acid from 5 to 200 ng/mL. The intra- and interday RSDs for all catecholamines were 1.0-8.0 and 2.1-14%, respectively. The method was successfully applied to determine the catecholamines in human urine from 14 Alzheimer's disease patients and 14 healthy volunteers. It was concluded that the mean levels of catecholamines in urine of Alzheimer's disease patients were all lower than those in healthy volunteers. The cluster analysis and independent samples T-test were used to distinguish the Alzheimer's disease patients and healthy volunteers.
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, P. R. China.
authors.forename: Lili
authors.initials: L
authors.lastname: Liu
authors.forename: Qing
authors.initials: Q
authors.lastname: Li
authors.forename: Naijing
authors.initials: N
authors.lastname: Li
authors.forename: Junhong
authors.initials: J
authors.lastname: Ling
authors.forename: Ran
authors.initials: R
authors.lastname: Liu
authors.forename: Yixiang
authors.initials: Y
authors.lastname: Wang
authors.forename: Lixin
authors.initials: L
authors.lastname: Sun
authors.forename: Xiao Hui
authors.initials: XH
authors.lastname: Chen
authors.forename: Kaishun
authors.initials: K
authors.lastname: Bi
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism / Q000652:urine*; D016022:Case-Control Studies; D002395:Catecholamines / Q000378:metabolism / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans
chemical_list: D002395:Catecholamines