title: Heat induces the splicing by IRE1 of a mRNA encoding a transcription factor involved in the unfolded protein response in Arabidopsis.
abstract: Adverse environmental conditions produce endoplasmic reticulum (ER) stress in plants. In response to heat or ER stress agents, Arabidopsis seedlings mitigate stress damage by activating ER-associated transcription factors and a RNA splicing factor, IRE1b. IRE1b splices the mRNA-encoding bZIP60, a basic leucine-zipper domain containing transcription factor associated with the unfolded protein response in plants. bZIP60 is required for the up-regulation of BINDING PROTEIN3 (BIP3) in response to ER stress, and loss-of-function mutations in IRE1b or point mutations in the splicing site of bZIP60 mRNA are defective in BIP3 induction. These findings demonstrate that bZIP60 in plants is activated by RNA splicing and afford opportunities for monitoring and modulating stress responses in plants.
authors.affiliation: Plant Sciences Institute, Iowa State University, Ames, IA 50011, USA.
authors.forename: Yan
authors.initials: Y
authors.lastname: Deng
authors.forename: Sabrina
authors.initials: S
authors.lastname: Humbert
authors.forename: Jian-Xiang
authors.initials: JX
authors.lastname: Liu
authors.forename: Renu
authors.initials: R
authors.lastname: Srivastava
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Rothstein
authors.forename: Stephen H
authors.initials: SH
authors.lastname: Howell
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D050976:Basic-Leucine Zipper Transcription Factors / Q000096:biosynthesis / Q000235:genetics; D017354:Point Mutation; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D012326:RNA Splicing / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism*; D036226:Seedlings / Q000235:genetics / Q000378:metabolism; D056811:Unfolded Protein Response / Q000502:physiology*
chemical_list: D029681:Arabidopsis Proteins; D050976:Basic-Leucine Zipper Transcription Factors; D012333:RNA, Messenger; D018749:RNA, Plant; C533092:bZIP60 protein, Arabidopsis; D011494:Protein Kinases; C446527:Ire1-1 protein, Arabidopsis