title: β-galactosidases from jack bean and Streptococcus have different cleaving abilities towards fucose-containing sugars.
abstract: We examined the sugar-cleaving abilities of β-galactosidases from jack bean and Streptococcus towards sugars containing fucose residues, and found that jack bean β-galactosidase has an ability to cleave the β1-3 linkage between galactose (Gal) and fucose (Fuc) residues, but not β1-4 linkage. On the other hand, streptococcal β-galactosidase was found to cleave the linkage in both Galβ1-4Fuc and Galβ1-3Fuc disaccharide units. Such a difference in sugar-cleaving abilities between these 2 β-galactosidases will be useful for structural analysis of glycans, especially those from species belonging to Protostomia, such as Caenorhabditis elegans.
authors.affiliation: Laboratory of Molecular Immunology, Faculty of Pharmaceutical Sciences, Josai University, Keyakidai, Sakado, Japan.
authors.forename: Tomoharu
authors.initials: T
authors.lastname: Takeuchi
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Sugiura
authors.forename: Kazusa
authors.initials: K
authors.lastname: Nishiyama
authors.forename: Hideyo
authors.initials: H
authors.lastname: Takahashi
authors.forename: Hideaki
authors.initials: H
authors.lastname: Natsugari
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Arata
authors.forename: Shunji
authors.initials: S
authors.lastname: Natsuka
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Kasai
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans; D004187:Disaccharides / Q000378:metabolism*; D007887:Fabaceae / Q000201:enzymology*; D005643:Fucose / Q000737:chemistry*; D005690:Galactose / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*; D013291:Streptococcus / Q000201:enzymology*; D013379:Substrate Specificity; D001616:beta-Galactosidase / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D004187:Disaccharides; D011134:Polysaccharides; D005643:Fucose; D001616:beta-Galactosidase; D005690:Galactose