title: Increased sensitivity of cholera toxin B treated K562 cells to natural killer cells.
abstract: Cholera toxin B-subunit (CTB) treatment of K562 erythroleukemia cells increased their sensitivity to be killed by NK-92 cells with more than 10%, compared to untreated cells. A similar treatment of non-T, non-B acute lymphoblastic REH leukemia cells, known to be unsensitive to NK cell mediated cytotoxicity, did not have any impact at all. Visualization of the cross-linked ganglioside(M1) (GM(1)) using fluorescent labeled CTB, indicated accumulation of the fluorescence to one cap and a few smaller patches in both type of cells. Additional cross-linking using anti-CTB antibodies further accentuated capping and increased lysis in the case of K562 cells. Blocking experiments performed with anti-MICA/B, ULBP-2 and/or CD59 antibodies could not inhibit the increased sensitivity mediated by CTB.
authors.affiliation: Department of Cell Biology, A Mickiewicz University, Poznań, Poland.
authors.forename: Lucyna
authors.initials: L
authors.lastname: Mrówczyńska
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Bobrowska-Hägerstrand
authors.forename: Henry
authors.initials: H
authors.lastname: Hägerstrand
authors.forename: Christer
authors.initials: C
authors.lastname: Lindqvist
mesh_terms: D002772:Cholera Toxin / Q000633:toxicity*; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D005434:Flow Cytometry; D006801:Humans; D020014:K562 Cells; D007694:Killer Cells, Natural / Q000276:immunology*
chemical_list: D002772:Cholera Toxin