title: Evaluation of ISSR markers for genetic diversity analysis in Anatolian water buffaloes.
abstract: BACKGROUND
  The utilisation of molecular markers has increased in molecular research recently. Inter-simple sequence repeat (ISSR) markers allow the analysis of genomes without preliminary sequence information, since random primers are used. The purpose of the present study was to evaluate ISSR markers for assessing the genetic diversity of indigenous Anatolian Water Buffaloes reared in Afyon, Konya and Sivas provinces of Turkey, with a view to conservation of the gene resources.
  
  
  RESULTS
  The 11 ISSR primers chosen for the analysis revealed a total of 110 bands, of which 76 (69.09%) were polymorphic. Also, genetic similarity, polymorphic information content (PIC), resolving power (R(p)) and mean resolving power (R(p)), heterozygosity (H) and Shannon index (I) were calculated as 0.9479-0.9562, 0.35 ± 0.20, 2.73, 0.27, 0.18 ± 0.07 and 0.28 ± 0.11, respectively.
  
  
  CONCLUSIONS
  The ISSR markers were found to be promising for assessing the genetic diversity in buffalo populations. Potential genetic parameters such as PIC, R(p), R(p), H and I were effectively used in this study.
authors.affiliation: Faculty of Agriculture, Department of Animal Science, University of Selcuk, Konya, Turkey. aytekin@selcuk.edu.tr
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Aytekin
authors.forename: Fulya
authors.initials: F
authors.lastname: Ozdil
authors.forename: Ugur
authors.initials: U
authors.lastname: Zulkadir
authors.forename: Saim
authors.initials: S
authors.lastname: Boztepe
authors.forename: Vahdettin
authors.initials: V
authors.lastname: Sariyel
mesh_terms: D000818:Animals; D002020:Buffaloes / Q000097:blood / Q000235:genetics*; D005819:Genetic Markers; D014644:Genetic Variation; D005828:Genetics, Population; D006579:Heterozygote; D020071:Interspersed Repetitive Sequences; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011110:Polymorphism, Genetic; D014421:Turkey
chemical_list: D005819:Genetic Markers