title: [Sequence analysis of the phosphoprotein gene of peste des petits ruminants virus of Chinese origin].
abstract: The nucleotide sequences of P gene from a field strain of peste des petits ruminants virus (PPRV) ("China/Tib/Gej/07-30") was firstly determined. The P gene is 1,655 nucleotides long with two overlapping open reading frames (ORFs). The first ORF is 1530 nucleotides long and would produce P protein of 509 amino acid residues. The second ORF is 534 nucleotides long and would produce C protein of 177 amino acid residues. The first ORF produces a second mRNA transcript of 897 nucleotides long with an extra G nucleotide at position 751. Translation from this mRNA would produce V protein of 298 amino acid residues. The nucleotide and deduced amino acid sequence were compared with the homologous region of other PPRV isolates. At the amino acid level, the "China/Tib/Gej/07-30" shares homology of 86.10%-97.3%, 84.3%-94.9%, and 82.9%-96.3% for P, C, and V proteins respectively. Several sequence motifs in the P genes were identified on the basis of conservation in the PPRVs and the morbilliviruses.
authors.affiliation: National Diagnostic Center for Exotic Animal Diseases, China Animal Health and Epidemiology Center, Qingdao 266032, China.
authors.forename: Jing-yue
authors.initials: JY
authors.lastname: Bao
authors.forename: Wen-ji
authors.initials: WJ
authors.lastname: Zhao
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.forename: Zhi-liang
authors.initials: ZL
authors.lastname: Wang
authors.forename: Guo-zhen
authors.initials: GZ
authors.lastname: Wu
authors.forename: Xiao-dong
authors.initials: XD
authors.lastname: Wu
authors.forename: Chun-ju
authors.initials: CJ
authors.lastname: Liu
authors.forename: Qing-hua
authors.initials: QH
authors.lastname: Wang
authors.forename: Jun-wei
authors.initials: JW
authors.lastname: Wang
authors.forename: Yu-tian
authors.initials: YT
authors.lastname: Liu
authors.forename: Jin-ming
authors.initials: JM
authors.lastname: Li
authors.forename: Ying-li
authors.initials: YL
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002681:China; D005260:Female; D015511:Goat Diseases / Q000821:virology*; D006041:Goats; D008969:Molecular Sequence Data; D029021:Peste-des-Petits-Ruminants / Q000662:veterinary* / Q000821:virology; D018107:Peste-des-petits-ruminants virus / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D010750:Phosphoproteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D010750:Phosphoproteins; D014764:Viral Proteins