title: Effect of Tris-HCl buffer on DNA adsorption by a variety of soil constituents.
abstract: We investigated the effect of tris(hydroxymethyl)aminomethane hydrochloride (Tris-HCl) buffer (pH 7.0) as a bulk solution on the adsorption of DNA by gibbsite, goethite, montmorillonite, kaolinite, synthetic and natural allophanes, two humic acids and two andosols. The natural allophane, gibbsite, kaolinite and an andosol adsorbed significantly more DNA in a 0.1 M Tris-HCl buffer than in a 0.1 M NaCl solution (t-test, P<0.005). In contrast, montmorillonite adsorbed significantly less DNA in the Tris-HCl than NaCl solution (P<0.05). Care should be taken when using Tris-HCl in studies on the adsorption of extracellular DNA molecules by soil particles.
authors.affiliation: Biotron Institute, Kyushu University, 6–10–1 Hakozaki, Higashi-ku, Fukuoka 812–8581, Japan.
authors.forename: Kazutoshi
authors.initials: K
authors.lastname: Saeki
authors.forename: Takashi
authors.initials: T
authors.lastname: Kunito
authors.forename: Masao
authors.initials: M
authors.lastname: Sakai
mesh_terms: D000327:Adsorption; D002021:Buffers; D004247:DNA / Q000737:chemistry* / Q000302:isolation & purification; D006812:Humic Substances / Q000032:analysis; D006851:Hydrochloric Acid / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D012987:Soil / Q000737:chemistry*; D014325:Tromethamine / Q000737:chemistry
chemical_list: D002021:Buffers; D006812:Humic Substances; D012987:Soil; D014325:Tromethamine; D004247:DNA; D006851:Hydrochloric Acid