title: Hybrid calcium phosphate coatings with the addition of trace elements and polyaspartic acid by a low-thermal process.
abstract: Research in the field of orthopedic implantology is currently focused on developing methodologies to potentiate osseointegration and to expedite the reestablishment of full functionality. We have developed a simple biomimetic approach for preparing trace elements-codoped calcium phosphate (teCaP) coatings on a titanium substrate. The reaction proceeded via low-thermal incubation in trace elements (TEs)-added simulated body fluid (teSBF) at 90 and 120 °C. The x-ray photoelectron spectroscopy, x-ray diffraction and energy-dispersive x-ray analyses demonstrated that the teCaP coating was the composite of hydroxyapatite and whitlockite, simultaneously doped with magnesium, strontium, zinc and silicon. The addition of polyaspartic acid and TEs into SBF significantly densified the coating. The incubation temperature is another important factor controlling the coating precipitation rate and bonding strength. An incubation temperature of 120 °C could accelerate the coating precipitation and improve the interface bonding strength. The in vitro cell culture investigation indicated that the teCaP coating supported the adhesion and spreading of ovariectomized rat mesenchymal stem cells (rMSCs) and particularly, promoted rMSCs proliferation compared to the CaP coating prepared in SBF. Collectively, from such a biomimetic route there potentially arises a general procedure to prepare a wide range of bioactive teCaP coatings of different composition for osteoporotic osteogenic cells activation response.
authors.affiliation: The First Affiliated Hospital, College of Medicine of Zhejiang University, Hangzhou 310003, People's Republic of China.
authors.forename: Sanzhong
authors.initials: S
authors.lastname: Xu
authors.forename: Xianyan
authors.initials: X
authors.lastname: Yang
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Chen
authors.forename: Xiangjin
authors.initials: X
authors.lastname: Lin
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Guojing
authors.initials: G
authors.lastname: Yang
authors.forename: Changyou
authors.initials: C
authors.lastname: Gao
authors.forename: Zhongru
authors.initials: Z
authors.lastname: Gou
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D032701:Biomimetics; D002130:Calcium Phosphates / Q000737:chemistry*; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods; D002470:Cell Survival; D005260:Female; D006358:Hot Temperature; D059630:Mesenchymal Stem Cells / Q000166:cytology; D016348:Osseointegration; D010455:Peptides / Q000032:analysis*; D051381:Rats; D014025:Titanium / Q000737:chemistry; D014131:Trace Elements / Q000032:analysis*
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; D010455:Peptides; D014131:Trace Elements; C017645:polyaspartate; C020243:calcium phosphate; D014025:Titanium