title: Protection against acoustic trauma by direct application of D-methionine to the inner ear.
abstract: CONCLUSIONS
  The findings from this study extend the use of the local application of D-methionine (D-met) to protect against acoustic trauma and demonstrate that D-met slowly diffuses from the perilymph.
  
  
  OBJECTIVE
  The objectives of the study were to determine the effect of D-met on auditory function and morphology after acoustic trauma and to measure the concentration of D-met in perilymph.
  
  
  METHODS
  Auditory thresholds were determine before, immediately after, and 24 h after acoustic trauma. Cochleae were analyzed using immunocytochemistry for c-Fos, TUJI, and cytochrome c. The concentration of D-met was determined from perilymph.
  
  
  RESULTS
  Protection against acoustic trauma (immediately and 24 h post trauma) on auditory brainstem thresholds was found at a time when the concentration of D-met in perilymph showed a fivefold increase above basal levels. The local application of D-met to the guinea pig cochlea results in elevated D-met concentrations that are maintained in the perilymph for at least 24 h.
authors.affiliation: Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Zlatan
authors.initials: Z
authors.lastname: Alagic
authors.forename: Michel
authors.initials: M
authors.lastname: Goiny
authors.forename: Barbara
authors.initials: B
authors.lastname: Canlon
mesh_terms: D000287:Administration, Topical; D000818:Animals; D001309:Auditory Threshold / Q000187:drug effects; D004195:Disease Models, Animal; D007758:Ear, Inner / Q000187:drug effects*; D005260:Female; D006168:Guinea Pigs; D006317:Hearing Loss, Noise-Induced / Q000503:physiopathology / Q000517:prevention & control*; D008715:Methionine / Q000008:administration & dosage*; D016896:Treatment Outcome
chemical_list: D008715:Methionine