title: Resting lymphocyte transduction with measles virus glycoprotein pseudotyped lentiviral vectors relies on CD46 and SLAM.
abstract: The measles virus (MV) glycoproteins hemagglutinin (H) and fusion (F) were recently shown to mediate transduction of resting lymphocytes by lentiviral vectors. MV vaccine strains use CD46 or signaling lymphocyte activation molecule (SLAM) as receptor for cell entry. A panel of H protein mutants derived from vaccine strain or wild-type MVs that lost or gained CD46 or SLAM receptor usage were investigated for their ability to mediate gene transfer into unstimulated T lymphocytes. The results demonstrate that CD46 is sufficient for efficient vector particle association with unstimulated lymphocytes. For stable gene transfer into these cells, however, both MV receptors were found to be essential.
authors.affiliation: Division of Medical Biotechnology, Paul-Ehrlich-Institut, Langen, Germany.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhou
authors.forename: Irene C
authors.initials: IC
authors.lastname: Schneider
authors.forename: Manuela
authors.initials: M
authors.lastname: Gallet
authors.forename: Sabrina
authors.initials: S
authors.lastname: Kneissl
authors.forename: Christian J
authors.initials: CJ
authors.lastname: Buchholz
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism*; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D015967:Gene Expression Regulation, Viral / Q000502:physiology; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics; D006388:Hemagglutinins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D016086:Lentivirus; D049408:Luminescent Agents; D008213:Lymphocyte Activation; D008459:Measles virus / Q000378:metabolism*; D051916:Membrane Cofactor Protein / Q000378:metabolism*; D009154:Mutation; D011956:Receptors, Cell Surface / Q000378:metabolism*; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1; D013601:T-Lymphocytes / Q000378:metabolism; D014760:Viral Fusion Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D015703:Antigens, CD; D006388:Hemagglutinins; D049408:Luminescent Agents; D051916:Membrane Cofactor Protein; D011956:Receptors, Cell Surface; D014760:Viral Fusion Proteins; D049452:Green Fluorescent Proteins; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1