title: Decoy strategy targeting the brain-derived neurotrophic factor exon I to attenuate tactile allodynia in the neuropathic pain model of rats.
abstract: The mechanism underlying neuropathic pain is still largely unclear. Recently, much attention has been focused on the role of brain-derived neurotrophic factor (BDNF) as a neuromodulator in the spinal cord. We previously reported that the expression of Bdnf exon I mRNA was remarkably up-regulated in the dorsal root ganglion (DRG) neurons with the rat L5 spinal nerve ligation (SNL) model. In the present study, we investigated whether neuropathic pain response would be reduced by the inhibition of the Bdnf exon I in the rat SNL model. We identified the promoter region of exon I and synthesized the decoy ODNs targeting the region. Reverse transcription-polymerase chain reaction analysis confirmed that the decoy ODN treatment reduced SNL-induced Bdnf exon I mRNA up-regulation in ipsilateral L4 and L5 DRGs. Furthermore, post-treatment with the decoy ODNs significantly attenuated SNL-induced tactile allodynia. This study suggested that decoy ODNs targeting the Bdnf exon I might provide a novel analgesic strategy for the treatment of neuropathic pain.
authors.affiliation: Department of Anesthesiology and Resuscitology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan. obatti@v102.vaio.ne.jp
authors.forename: Norihiko
authors.initials: N
authors.lastname: Obata
authors.forename: Satoshi
authors.initials: S
authors.lastname: Mizobuchi
authors.forename: Yoshitaro
authors.initials: Y
authors.lastname: Itano
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Matsuoka
authors.forename: Ryuji
authors.initials: R
authors.lastname: Kaku
authors.forename: Naoto
authors.initials: N
authors.lastname: Tomotsuka
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Morita
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Kanzaki
authors.forename: Mamoru
authors.initials: M
authors.lastname: Ouchida
authors.forename: Masataka
authors.initials: M
authors.lastname: Yokoyama
mesh_terms: D000818:Animals; D001483:Base Sequence; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics*; D004195:Disease Models, Animal; D005091:Exons / Q000235:genetics*; D055785:Gene Knockdown Techniques; D006930:Hyperalgesia / Q000628:therapy*; D008297:Male; D008969:Molecular Sequence Data; D009437:Neuralgia / Q000628:therapy*; D009841:Oligonucleotides / Q000235:genetics; D051381:Rats; D013127:Spinal Nerves / Q000503:physiopathology
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D009841:Oligonucleotides