title: Simple method for detection of extremely diluted anti beta-casein antibodies from glass bead based receptors.
abstract: The current work describes the elaboration of a simple, sensitive and reliable β-casein modified glass beads, for the detection and quantification of its specific antibody anti β-casein. This is an elementary receptor without electronic part, developed by grafting glass bead surface with the antigenic β-casein via 3-aminopropyltriethoxy silane and then glutaraldehyde as cross-linker. The whole is realized by a classical process, called in two steps and in mild conditions where chemical protocol is optimized for β-casein use. The detection and quantification of the specific reaction antibody-receptor is carried out by the technique of the second antibody labeled with horse radish peroxidase (HRP). Our receptor can detect the β-casein antibody present in the serum at dilutions up to a factor 10(7) in strong ionic strength medium. The same antibody of the same serum and in the same conditions can be detected by ELISA test at dilutions up to a factor 10(5). The whole test, after our receptor realization, takes about 5h.
authors.affiliation: Technological Institute of Larissa, Department of Medical Laboratories, TEI of Larissa, T.K. 411 10 Larissa, Greece. antalex@teilar.gr
authors.forename: Alexandros
authors.initials: A
authors.lastname: Antoniou
authors.forename: Guillaume
authors.initials: G
authors.lastname: Herlem
authors.forename: Claire
authors.initials: C
authors.lastname: André
authors.forename: Yves
authors.initials: Y
authors.lastname: Guillaume
authors.forename: Tijani
authors.initials: T
authors.lastname: Gharbi
mesh_terms: D000906:Antibodies / Q000032:analysis*; D002364:Caseins / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006735:Horseradish Peroxidase / Q000378:metabolism
chemical_list: D000906:Antibodies; D002364:Caseins; D006735:Horseradish Peroxidase