title: Studies on immobilization and partial characterization of lipases from wheat seeds (Triticum aestivum).
abstract: The objective of this study was to provide some features on immobilization and partial characterization of lipases from wheat seeds. The optimum pH and temperature were found to be 5.5 and 32-37 °C, respectively. The stability of the concentrated enzymatic extract to high temperatures (25, 35, 45, and 55 °C) showed that the incubation of the extract at 55 °C led to its complete inactivation. The concentrated enzymatic extract kept 90% of its hydrolytic and esterification activities until 70 and 40 days of storage at 4 °C, respectively. The extract presented higher hydrolytic specificity to substrates of medium and long chains and higher esterification affinity to fatty acids of short and medium chains and alcohols with two and three carbon atoms. After the immobilization process using activated coal and sodium alginate as supports, an enhancement of about threefold in lipase activity was observed. The development of the present work permitted us to point out some characteristics of lipases from wheat seeds necessary for the proposition of new future industrial applications for this important biocatalyst.
authors.affiliation: Universidade Federal do Rio de Janeiro, Centro de Tecnologia, Av. Athos da Silveira Ramos, 149-Bloco A, Rio de Janeiro, 21941-909, Rio de Janeiro, Brazil.
authors.forename: Morgana Karin
authors.initials: MK
authors.lastname: Pierozan
authors.forename: Enrique Guillermo
authors.initials: EG
authors.lastname: Oestreicher
authors.forename: J Vladimir
authors.initials: JV
authors.lastname: Oliveira
authors.forename: Débora
authors.initials: D
authors.lastname: Oliveira
authors.forename: Helen
authors.initials: H
authors.lastname: Treichel
authors.forename: Rogério Luís
authors.initials: RL
authors.lastname: Cansian
mesh_terms: D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D008049:Lipase / Q000737:chemistry / Q000378:metabolism*; D012639:Seeds / Q000201:enzymology*; D013696:Temperature; D014908:Triticum / Q000201:enzymology*
chemical_list: D004800:Enzymes, Immobilized; D008049:Lipase