title: Primary culture of neurospheres obtained from fetal mouse central nervous system and generation of inner ear hair cell immunophenotypes in vitro.
abstract: OBJECTIVE
  To investigate whether hair cell immunophenotypes can be derived from the central nervous system.
  
  
  METHODS
  We established in vitro cell cultures from embryonic day 14.5 fetal rat brain tissue, and analysed changes in the immunohistochemical features of these cell cultures following differentiation.
  
  
  RESULTS
  The immature neural progenitors obtained from the fetal mouse central nervous system generated cell immunophenotypes which expressed epitopes of the hair cell marker proteins myosin VIIa and Brn-3c and the supporting cell marker pan-cytokeratin.
  
  
  CONCLUSIONS
  Neural progenitors have the potential to differentiate into inner ear hair cell and supporting cell phenotypes, and thus may be a useful material for cell transplantation therapy aiming to replace damaged inner ear hair cells.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Anhui Provincial Hospital, Hefei, Anhui Province, China.
authors.forename: X-W
authors.initials: XW
authors.lastname: Liu
authors.forename: J-W
authors.initials: JW
authors.lastname: Sun
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology / Q000196:embryology; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000502:physiology*; D054338:Cell Transdifferentiation / Q000502:physiology*; D002478:Cells, Cultured; D002490:Central Nervous System / Q000166:cytology*; D005260:Female; D005333:Fetus; D006199:Hair Cells, Auditory, Inner / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D016130:Immunophenotyping / Q000379:methods; D007381:Intermediate Filament Proteins / Q000378:metabolism; D007633:Keratins / Q000378:metabolism; D008297:Male; D051379:Mice; D000081412:Myosin VIIa; D009218:Myosins / Q000378:metabolism; D009416:Nerve Regeneration / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000378:metabolism; D064231:Nestin; D058953:Neural Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D051381:Rats; D050818:Transcription Factor Brn-3 / Q000378:metabolism
chemical_list: D007381:Intermediate Filament Proteins; C000631456:Myo7a protein, mouse; C000631457:Myo7a protein, rat; D000081412:Myosin VIIa; D009419:Nerve Tissue Proteins; C577485:Nes protein, mouse; C577486:Nes protein, rat; D064231:Nestin; D050818:Transcription Factor Brn-3; D007633:Keratins; D009218:Myosins