title: Olfactomedin 4 suppresses prostate cancer cell growth and metastasis via negative interaction with cathepsin D and SDF-1.
abstract: The human olfactomedin 4 gene (OLFM4) encodes an olfactomedin-related glycoprotein. OLFM4 is normally expressed in a limited number of tissues, including the prostate, but its biological functions in prostate are largely unknown. In this study, we found that OLFM4 messenger RNA was reduced or undetectable in prostate cancer tissues and prostate cancer cell lines. To study the effects of OLFM4 on prostate cancer progression, we transfected PC-3 prostate cancer cells with OLFM4 to establish OLFM4-expressing PC-3 cell clones. The OLFM4-expressing PC-3 cell clones were found to have decreased proliferation and invasiveness compared with vector-transfected control PC-3 cells in vitro. In addition, nude mice injected with OLFM4-expressing PC-3 cells demonstrated reduced tumor growth and bone invasion and metastasis compared with mice injected with vector-transfected control cells. Mechanistic studies revealed that OLFM4 may exhibit its anticancer effects through regulating cell autophagy by targeting cathepsin D, as OLFM4 reduced cathepsin D protein levels and enzymatic activity and attenuated cathepsin D-induced cancer cell proliferation. In addition, overexpression of OLFM4 abrogated stromal cell derived factor-1 (SDF-1)-induced PC-3 cell invasiveness in a Matrigel invasion assay, partially through blocking SDF-1-mediated AKT phosphorylation. Coimmunoprecipitation and immunofluorescence staining studies in OLFM4-expressing PC-3 cells demonstrated a direct interaction between OLFM4 and cathepsin D or SDF-1. Taken together, these results suggest that OLFM4 negatively interacts with cathepsin D and SDF-1 and inhibits prostate cancer growth and bone metastasis.
authors.affiliation: Molecular and Clinical Hematology Branch, National Heart, Lung and Blood Institute, Bethesda, MD 20892, USA.
authors.forename: Ling
authors.initials: L
authors.lastname: Chen
authors.forename: Hongzhen
authors.initials: H
authors.lastname: Li
authors.forename: Wenli
authors.initials: W
authors.lastname: Liu
authors.forename: Jianqiong
authors.initials: J
authors.lastname: Zhu
authors.forename: Xiongce
authors.initials: X
authors.lastname: Zhao
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Wright
authors.forename: Liu
authors.initials: L
authors.lastname: Cao
authors.forename: Ivan
authors.initials: I
authors.lastname: Ding
authors.forename: Griffin P
authors.initials: GP
authors.lastname: Rodgers
mesh_terms: D002402:Cathepsin D / Q000378:metabolism*; D002455:Cell Division; D054377:Chemokine CXCL12 / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005434:Flow Cytometry; D016179:Granulocyte Colony-Stimulating Factor / Q000502:physiology*; D006801:Humans; D008297:Male; D009362:Neoplasm Metastasis; D011471:Prostatic Neoplasms / Q000378:metabolism / Q000473:pathology*
chemical_list: D054377:Chemokine CXCL12; C454127:OLFM4 protein, human; D016179:Granulocyte Colony-Stimulating Factor; D002402:Cathepsin D