title: Effects of fenofibrate on Semaphorin 6B gene expression in rat skeletal muscle.
abstract: Semaphorin 6B is a member of the semaphorin family of signaling proteins, which is implicated in a variety of biological processes, such as axon guidance and muscle regeneration. PPARα is known to be a regulator of semaphorin 6B in human cancer cell lines. In this study, we examined the effect of fenofibrate as a PPARα activator on the expression of the Sema6B gene in rat skeletal muscle. A total of 18 rats were divided into two groups: one fed with standard chow (control group), and the other with fenofibrate chow (treatment group) for 4 weeks by gavage with 30 mg/kg per day. The expression of rSema6B mRNA was analyzed by qPCR. rSema6B protein content in rat skeletal muscle was measured by Western blotting. A significant down-regulation of rSema6B was observed at both the mRNA and protein levels. Using the ChIP assay, a putative PPARα binding region (PPRE) was identified in the rSema6B promoter. We conclude that the expression of Sema6B is down-regulated by fenofibarte in rat skeletal muscle.
authors.affiliation: Department of Molecular Biology and Biotechnology and Division of Human Genetics, Atomic Energy Commission of Syria, 6091 Damascus, Syria.
authors.forename: Hossam
authors.initials: H
authors.lastname: Murad
authors.forename: Fadia
authors.initials: F
authors.lastname: Kasies
authors.forename: Raed
authors.initials: R
authors.lastname: Azroony
authors.forename: Ghassan
authors.initials: G
authors.lastname: Alya
authors.forename: Ammar
authors.initials: A
authors.lastname: Madania
mesh_terms: D000818:Animals; D047369:Chromatin Immunoprecipitation; D019295:Computational Biology; D005260:Female; D011345:Fenofibrate / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000378:metabolism; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism; D047492:Peroxisome Proliferator-Activated Receptors / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D020218:Response Elements / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D047492:Peroxisome Proliferator-Activated Receptors; D012333:RNA, Messenger; C110238:Sema6b protein, rat; D011345:Fenofibrate