title: [Genetic similarity of vancomycin resistant strains of Enterococcus faecium isolated from clinical specimens].
abstract: Twenty vancomycin resistant E. faecium strains (VRE) isolated from patients of three different hospital wards in 2005-2008 were examined. The strains originated from patients of intensive therapy, urological and internistic wards. The chosen wards differ significantly in their specificity. In all cases the presence of o vanA and lack of vanB, vanD, vanE and vanG genes and were found. Strains were compared by using RFLP-PFGE, the reference method for molecular typing of VRE. One group including fourteen strains showing similarity higher than 79.5% was distinguished. This group was divided into subgroups. The greatest similarity was found among strains from patients of intensive therapy ward. Two subgroups of strains showing similarity more than 93.3%, of four strains each were identified. The similarity between these two subgroups was 79.5%. Most strains from other two wards showed less than 79.5% similarity and they could be recognised as not related. Only one strain from internal ward and two strains from urologic ward were similar in 82.1 - 86.4% to one of subgroups of strains originated from intensive therapy.
authors.affiliation: Katedra i Zakład Mikrobiologii Lekarskiej Warszawskiego Uniwersytetu Medycznego.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Młynarczyk
authors.forename: Wanda
authors.initials: W
authors.lastname: Grzybowska
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Mrówka
authors.forename: Stefan
authors.initials: S
authors.lastname: Tyski
authors.forename: Grazyna
authors.initials: G
authors.lastname: Młynarczyk
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D019729:Carbon-Oxygen Ligases / Q000235:genetics*; D016984:Enterococcus faecium / Q000145:classification / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics*; D058889:Molecular Typing; D020713:Vancomycin Resistance / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; C071437:VanA ligase, Bacteria; D019729:Carbon-Oxygen Ligases