title: [Role of NGX6 in Wnt/β-catenin signaling pathway in colon cancer].
abstract: OBJECTIVE
  To explore the role of nasopharyngeal carinoma associated gene 6 (NGX6) in Wnt/β-catenin signaling pathway and to identify whether there is feedback regulation between NGX6 and Wnt signal pathway once the Wnt signal is blocked.
  
  
  METHODS
  pCMV-myc-NGX6 and pCMV-myc were transfected to colon cancer cell line SW62O and the expression of downstream target molecules of Wnt/β-catenin signaling pathway such as c-myc, cyclinD1, and c-jun were detected by Western blot. The eukaryotic expression vector pcDNA3.1 (+)-LEF1(DN) (without N-terminal β-catenin binding domain) was constructed to inhibit Wnt signaling pathway, and the effect was confirmed by Western blot and TCF-4 luciferase report system. The mRNA expression of NGX6 in SW620 was detected by RT-PCR after pcDNA3.1(+)-LEF1(DN) transient transfection.
  
  
  RESULTS
  The expression of above molecules were obviously down-regulated by NGX6 transient transfection in SW620. The eukaryotic expression vector pcDNA3.1(+)-LEF1(DN) showed an inhibitory effect on Wnt/β-catenin signaling pathway. The mRNA expression of NGX6 in SW620 was up-regulated after pcDNA3.1(+)-LEF1(DN) transient transfection.
  
  
  CONCLUSIONS
  NGX6 could inhibit the expression of Wnt signaling downstream target genes, which maybe related to the potential feedback regulation between NGX6 and Wnt signal pathway.
authors.affiliation: Department of Digestion Medicine, Third Xiangya Hospital, Central South University, Changsha 410013, China.
authors.forename: Fen
authors.initials: F
authors.lastname: Liu
authors.forename: Shourong
authors.initials: S
authors.lastname: Sheng
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Wang
authors.forename: Ya
authors.initials: Y
authors.lastname: Peng
authors.forename: Qin
authors.initials: Q
authors.lastname: Guo
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Xiao
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
mesh_terms: D001483:Base Sequence; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D019938:Cyclin D1 / Q000235:genetics; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014162:Transfection; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*; D060449:Wnt Signaling Pathway / Q000235:genetics*
chemical_list: C530104:CCND1 protein, human; C489427:MYC protein, human; D008565:Membrane Proteins; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; C477325:TMEM8B protein, human; D025521:Tumor Suppressor Proteins; D019938:Cyclin D1