title: Reduction of 8-iso-prostaglandin F2α in the first week after Roux-en-Y gastric bypass surgery.
abstract: Obesity is associated with increased markers of oxidative stress. We examined whether oxidative stress is reduced within the first week after Roux-en-Y gastric bypass (RYGB) surgery and could be related to changes in adipose tissue depots. The reactive oxygen species (ROS) marker 8-iso-prostaglandin F2α (8-iso-PGF2α) and activity of antioxidant glutathione peroxidases (GPX) in plasma were compared before and ~1 week after RYGB. The effects of RYGB on subcutaneous adipose tissue and interstitial fluid 8-iso-PGF2α levels and subcutaneous adipose tissue expression of GPX-3 were also assessed. Levels of 8-iso-PGF2α in subcutaneous and visceral adipose tissue were determined. Plasma 8-iso-PGF2α levels decreased (122 ± 75 to 56 ± 15 pg/ml, P = 0.001) and GPX activity increased (84 ± 18 to 108 ± 25 nmol/min/ml, P = 0.003) in the first week post-RYGB. RYGB also resulted in reductions of 8-iso-PGF2α in subcutaneous adipose tissue (1,742 ± 931 to 1,132 ± 420 pg/g fat, P = 0.046) and interstitial fluid (348 ± 118 to 221 ± 83 pg/ml, P = 0.046) that were comparable to plasma (26-33%, P = 0.74). Adipose GPX-3 expression was increased (6.7 ± 4.7-fold, P = 0.004) in the first postoperative week. The improvements in oxidative stress occurred with minimal weight loss (2.4 ± 3.4%, P = 0.031) and elevations in plasma interleukin-6 (18.0 ± 46.8 to 28.0 ± 58.9 pg/ml, P = 0.004). Subcutaneous and visceral adipose tissues express comparable 8-iso-PGF2α levels (1,204 ± 470 and 1,331 ± 264 pg/g fat, respectively; P = 0.34). These data suggest that RYGB affects adipose tissue leading to the restoration of adipose redox balance within the first postoperative week and that plasma 8-iso-PGF2α is primarily derived from subcutaneous adipose tissue.
authors.affiliation: Department of Surgery, Vanderbilt University School of Medicine, Nashville, Tennessee, USA.
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Ueda
authors.forename: Tahar
authors.initials: T
authors.lastname: Hajri
authors.forename: DunFa
authors.initials: D
authors.lastname: Peng
authors.forename: Pamela A
authors.initials: PA
authors.lastname: Marks-Shulman
authors.forename: Robyn A
authors.initials: RA
authors.lastname: Tamboli
authors.forename: Bassam
authors.initials: B
authors.lastname: Shukrallah
authors.forename: Jabbar
authors.initials: J
authors.lastname: Saliba
authors.forename: Kareem
authors.initials: K
authors.lastname: Jabbour
authors.forename: Wael
authors.initials: W
authors.lastname: El-Rifai
authors.forename: Nada A
authors.initials: NA
authors.lastname: Abumrad
authors.forename: Naji N
authors.initials: NN
authors.lastname: Abumrad
mesh_terms: D015237:Dinoprost / Q000097:blood / Q000378:metabolism*; D045604:Extracellular Fluid / Q000378:metabolism*; D015390:Gastric Bypass; D005979:Glutathione Peroxidase / Q000378:metabolism; D006801:Humans; D015850:Interleukin-6 / Q000097:blood; D050152:Intra-Abdominal Fat / Q000378:metabolism*; D009765:Obesity / Q000378:metabolism* / Q000601:surgery; D018384:Oxidative Stress; D011184:Postoperative Period; D050151:Subcutaneous Fat / Q000378:metabolism*; D015431:Weight Loss
chemical_list: D015850:Interleukin-6; D015237:Dinoprost; D005979:Glutathione Peroxidase