title: Effect of acupuncture on rats with acute gouty arthritis inflammation: a metabonomic method for profiling of both urine and plasma metabolic perturbation.
abstract: Acute gouty arthritis is a common inflammation model with multiple pathogenic mechanisms seen in clinical practice, for which acupuncture may potentially be an alternative therapy. To investigate the effect of acupuncture on acute gouty arthritis and search for its mechanism, a metabonomic method was developed in this investigation. Acute gouty arthritis model rats were induced by monosodium urate (MSU) crystals. The urine and plasma samples were collected at several time points and the endogenous metabolites were analyzed by an ultra-performance liquid chromatography coupled with a mass spectrometry (UPLC-MS). Data were analyzed using principal components analysis (PCA) and partial least squares (PLS) analysis to compare metabolic profiles of MSU crystal-induced acute gouty arthritis rats with MSU crystal-induced acute gouty arthritis, treated with acupuncture rats. The results showed that acupuncture could restore the metabolite network that disturbed by MSU administration. Our study indicates that UPLC-MS-based metabonomics can be used as a potential tool for the investigation of biological effect of acupuncture on acute gouty arthritis.
authors.affiliation: West China School of Pharmacy, Sichuan University, No. 17 South Renmin Road, Chengdu, P. R. China.
authors.forename: Si-Lan
authors.initials: SL
authors.lastname: Wen
authors.forename: Yu-Jie
authors.initials: YJ
authors.lastname: Liu
authors.forename: Hai-Lin
authors.initials: HL
authors.lastname: Yin
authors.forename: Liu
authors.initials: L
authors.lastname: Zhang
authors.forename: Jin
authors.initials: J
authors.lastname: Xiao
authors.forename: Hong-Yuan
authors.initials: HY
authors.lastname: Zhu
authors.forename: Jin-Tao
authors.initials: JT
authors.lastname: Xue
authors.forename: Li-Ming
authors.initials: LM
authors.lastname: Ye
mesh_terms: D015670:Acupuncture Therapy; D000818:Animals; D015210:Arthritis, Gouty / Q000097:blood / Q000378:metabolism* / Q000628:therapy / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D004195:Disease Models, Animal; D007249:Inflammation / Q000097:blood / Q000378:metabolism* / Q000628:therapy / Q000652:urine; D016018:Least-Squares Analysis; D008297:Male; D013058:Mass Spectrometry; D055442:Metabolome; D055432:Metabolomics / Q000379:methods*; D025341:Principal Component Analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D016896:Treatment Outcome; D014527:Uric Acid
chemical_list: D014527:Uric Acid