title: Determination of stilbene derivatives in Burgundy red wines by ultra-high-pressure liquid chromatography.
abstract: The polyphenols, for example stilbenes and flavonoids, are an important family of compounds present in grapes and wines. Several studies have shown that stilbenes are antioxidants and cancer-preventing agents. For the first time, eight natural stilbenes (trans-resveratrol, trans-piceid, cis-piceid, trans-astringin, trans-piceatannol, (+)-trans-ε-viniferin, pallidol, and hopeaphenol), isolated and purified from Vitis vinifera, were simultaneously analysed by ultra-high-pressure liquid chromatography coupled with photodiode-array detection. Separation of the stilbenes by UHPLC was optimized with the assistance of "Quality-by-Design" commercial software. Four different reversed-phase columns packed with 1.5-1.7-μm particles were tested and compared for their retention behaviour and separation efficiency. On the basis of the performance characteristics determined, the VisionHT C18 HL column was selected for the stilbenes studied, because resolution of the critical pair was 1.5 with a peak width of 2-4 s. The optimized method resulted in highly repeatable retention times (RSD 0.03-0.07%), peak areas (RSD 3-6%), and linear ranges were between 0.005 and 50 mg L(-1) for most of the compounds. All stilbenes, except trans-astringin, trans-piceatannol, and pallidol were identified and quantified in Burgundy red wines at different concentrations after direct injection of the wines.
authors.affiliation: EA 581 EMMA, Institut Universitaire de la vigne et du vin-Jules Guyot, Université de Bourgogne, Rue Claude Ladrey, 21078 Dijon, France.
authors.forename: Lemia
authors.initials: L
authors.lastname: Boutegrabet
authors.forename: Agnes
authors.initials: A
authors.lastname: Fekete
authors.forename: Norbert
authors.initials: N
authors.lastname: Hertkorn
authors.forename: Yorgos
authors.initials: Y
authors.lastname: Papastamoulis
authors.forename: Pierre
authors.initials: P
authors.lastname: Waffo-Téguo
authors.forename: Jean Michel
authors.initials: JM
authors.lastname: Mérillon
authors.forename: Philippe
authors.initials: P
authors.lastname: Jeandet
authors.forename: Régis D
authors.initials: RD
authors.lastname: Gougeon
authors.forename: Philippe
authors.initials: P
authors.lastname: Schmitt-Kopplin
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013267:Stilbenes / Q000032:analysis* / Q000302:isolation & purification; D014920:Wine / Q000032:analysis*
chemical_list: D013267:Stilbenes