title: Characterization of TioQ, a type II thioesterase from the thiocoraline biosynthetic cluster.
abstract: An antitumor agent thiocoraline is a thiodepsipeptide marine product derived from two Micromonospora sp. strains that inhibits protein synthesis by binding of its key 3-hydroxyquinaldic acid (3HQA) chromophores to duplex DNA. There are at least two potential pathways via which the 3HQA moiety could be biosynthesized from L-Trp. By biochemical characterization and by preparation of knockouts of an adenylation-thiolation enzyme, TioK, and of two type II thioesterases, TioP and TioQ, found in the thiocoraline biosynthetic gene cluster, we gained valuable insight into the pathway followed for the production of 3HQA.
authors.affiliation: University of Michigan, Life Sciences Institute, 210 Washtenaw Ave, Ann Arbor, MI 48109, USA.
authors.forename: Ahmed S A
authors.initials: AS
authors.lastname: Mady
authors.forename: Olga E
authors.initials: OE
authors.lastname: Zolova
authors.forename: María Álvarez San
authors.initials: MÁ
authors.lastname: Millán
authors.forename: Germán
authors.initials: G
authors.lastname: Villamizar
authors.forename: Fernando
authors.initials: F
authors.lastname: de la Calle
authors.forename: Felipe
authors.initials: F
authors.lastname: Lombó
authors.forename: Sylvie
authors.initials: S
authors.lastname: Garneau-Tsodikova
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D003065:Coenzyme A / Q000737:chemistry; D047630:Depsipeptides / Q000096:biosynthesis* / Q000235:genetics; D057075:Enzyme Assays; D064429:Fatty Acid Synthases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D055786:Gene Knockout Techniques; D006868:Hydrolysis; D008969:Molecular Sequence Data; D005810:Multigene Family; D010446:Peptide Fragments / Q000737:chemistry; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics*; D013379:Substrate Specificity; D013869:Thiolester Hydrolases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D047630:Depsipeptides; D010446:Peptide Fragments; D011994:Recombinant Proteins; C109005:thiocoraline; D064429:Fatty Acid Synthases; D013869:Thiolester Hydrolases; C022738:thioesterase II; D003065:Coenzyme A