title: Protective effect of Renshen Yangrong Decoction (人参养荣汤) on bone marrow against radiation injury in mouse.
abstract: OBJECTIVE
  To explore the effect of Renshen Yangrong Decoction (人参养荣汤, RYD) in protecting bone marrow from radiation injury.
  
  
  METHODS
  One hundred and eighty Kuming mice were subjected to the three tests for anti-radiation injury effect evaluation, i.e. the test of peripheral white blood cell (WBC) count, the test of bone marrow nucleated cell count, and the bone marrow micronucleus test, using 60 mice for each test. The mice in each test were divided into 6 groups: the blank control group, the model control group, the positive control group treated by Shiyiwei Shenqi Tablet (十一味参芪片, 1.0 g/kg), and three RYD groups treated with high (42.0 g/kg), moderate (21.0 g/kg), and low (10.5 g/kg) doses of crude drugs of RYD, with 10 mice in each group. The treatment was given by gastrogavage perfusion continuously for 7-14 days before mice received (60)Co-γ ray radiation and continued until the end of the experiment. The body weights of the mice were monitored, the changes in peripheral WBC and bone marrow nucleated cells were counted, and the variation in bone marrow micronucleated cells was observed on the respective appointed days.
  
  
  RESULTS
  A significant decrease in body weight, peripheral WBC count, and bone marrow nucleated cell count, as well as marked changes in bone marrow micronucleated cells were observed in the mice after radiation, indicating that the radiation injury model was successfully established. As compared with the model control group, the decrease in body weight, peripheral WBC count, and bone marrow nucleated cell count, as well as the increase in bone marrow micronucleus cell count in the high dosage RYD treated group were obviously inhibited or lessened (P<0.05 or P<0.01).
  
  
  CONCLUSIONS
  RYD showed obvious protective effect in mice with bone marrow injury induced by radiation.
authors.affiliation: Key laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Integration of Traditional Chinese and Western Medicine, Peking University School of Oncology, Beijing Cancer Hospital & Institute, Beijing, China.
authors.forename: Yan-zhi
authors.initials: YZ
authors.lastname: Chen
authors.forename: Fei
authors.initials: F
authors.lastname: Lin
authors.forename: Gui-bao
authors.initials: GB
authors.lastname: Zhuang
authors.forename: Yan
authors.initials: Y
authors.lastname: Ren
authors.forename: Ping-ping
authors.initials: PP
authors.lastname: Li
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects / Q000528:radiation effects; D001853:Bone Marrow / Q000187:drug effects* / Q000473:pathology / Q000528:radiation effects*; D001854:Bone Marrow Cells / Q000187:drug effects / Q000473:pathology / Q000528:radiation effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D007958:Leukocyte Count; D051379:Mice; D011832:Radiation Injuries / Q000097:blood / Q000473:pathology / Q000517:prevention & control*; D011837:Radiation-Protective Agents / Q000494:pharmacology*
chemical_list: D004365:Drugs, Chinese Herbal; D011837:Radiation-Protective Agents