title: Protective effect of ascorbic acid on netilmicin-induced lipid profile and peroxidation parameters in rabbit blood plasma.
abstract: A drug may cause alteration in blood-lipid profile and induce lipid peroxidation phenomena on administration in the body. Antioxidant may play beneficial role to control the negative alteration in lipid profile and lipid peroxidation. In view of this context, the present in vivo study was carried out to evaluate the role of ascorbic acid as antioxidant on netilmicin-induced alteration of blood lipid profile and peroxidation parameters. Rabbits were used as experimental animals and blood was collected to estimate blood-lipid profiles, such as total cholesterol (TCh), high density lipoprotein cholesterol (HDL-Ch), low density lipoprotein cholesterol (LDL-Ch), very low density lipoprotein cholesterol (VLDL-Ch), triglycerides (Tg), phospholipids (PL), and total lipids (TL), as well as peroxidation parameters, such as malondialdehyde (MDA), 4-hydroxy-2-nonenal (HNE), reduced glutathione (GSH) and nitric oxide (NO). The results revealed that netilmicin caused significant enhancement of MDA, HNE, TCh, LDL-Ch, VLDL-Ch, Tg levels and reduction in GSH, NO, HDL-Ch, PL, TL levels. On co-administration, ascorbic acid was found to be effective in reducing netilmicin-induced negative alterations of the above parameters.
authors.affiliation: Division of Medicinal and Pharmaceutical Chemistry, Dept. of Pharmaceutical Technology, Jadavpur University, Kolkata-700032, India. priteshdevbhuti@yahoo.co.in
authors.forename: Pritesh
authors.initials: P
authors.lastname: Devbhuti
authors.forename: Debasis
authors.initials: D
authors.lastname: Sikdar
authors.forename: Achintya
authors.initials: A
authors.lastname: Saha
authors.forename: Chandana
authors.initials: C
authors.lastname: Sengupta
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000633:toxicity*; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology*; D015415:Biomarkers / Q000097:blood; D050356:Lipid Metabolism / Q000187:drug effects*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008055:Lipids / Q000097:blood*; D009428:Netilmicin / Q000633:toxicity*; D011817:Rabbits; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D000975:Antioxidants; D015415:Biomarkers; D008055:Lipids; D009428:Netilmicin; D001205:Ascorbic Acid