title: Susceptibility of clinical Staphylococcus aureus isolates to innate defense antimicrobial peptides.
abstract: Antimicrobial peptides (AMPs) are effector molecules of innate immunity. To determine whether AMP susceptibility of S. aureus varies according to different types of infection, 102 isolates from patients with S. aureus bacteremia or recurrent skin and soft tissue infection, and colonizing isolates were investigated. Using microbroth dilution assays we found a narrow range of MICs of human β-defensin-3, cathelicidin LL-37 and bovine indolicidin without significant differences between the groups. Colony-forming unit (CFU) assays revealed minor differences in bactericidal activity with slightly but not significantly higher CFU reduction in colonizing isolates. These data do not support a role for differential AMP susceptibility in vitro as a major determinant of S. aureus invasive infection.
authors.affiliation: Center for Infectious Diseases and Travel Medicine, Department of Medicine, University Hospital, Hugstetter Strasse 55, D-79106 Freiburg, Germany. siegbert.rieg@uniklinik-freiburg.de
authors.forename: Siegbert
authors.initials: S
authors.lastname: Rieg
authors.forename: Achim J
authors.initials: AJ
authors.lastname: Kaasch
authors.forename: Julian
authors.initials: J
authors.lastname: Wehrle
authors.forename: Silke C
authors.initials: SC
authors.lastname: Hofmann
authors.forename: Magdalena
authors.initials: M
authors.lastname: Szymaniak-Vits
authors.forename: Viola
authors.initials: V
authors.lastname: Saborowski
authors.forename: Daniel
authors.initials: D
authors.lastname: Jonas
authors.forename: Hubert
authors.initials: H
authors.lastname: Kalbacher
authors.forename: Harald
authors.initials: H
authors.lastname: Seifert
authors.forename: Winfried V
authors.initials: WV
authors.lastname: Kern
mesh_terms: D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000276:immunology* / Q000494:pharmacology; D016470:Bacteremia / Q000276:immunology / Q000382:microbiology; D002417:Cattle; D015169:Colony Count, Microbial; D054884:Host-Pathogen Interactions; D006801:Humans; D008826:Microbial Sensitivity Tests; D018461:Soft Tissue Infections / Q000276:immunology / Q000382:microbiology; D013203:Staphylococcal Infections / Q000276:immunology / Q000382:microbiology*; D013207:Staphylococcal Skin Infections / Q000276:immunology / Q000382:microbiology; D013211:Staphylococcus aureus / Q000187:drug effects / Q000302:isolation & purification*
chemical_list: D023181:Antimicrobial Cationic Peptides