title: Three new isoflavone glycosides from Tongmai granules.
abstract: Three new isoflavone glycosides, 3'-methoxydaidzein-7,4'-di-O-β-d-glucopyranoside (1), biochanin A-8-C-β-d-apiofuranosyl-(1 → 6)-O-β-d-glucopyranoside (2), daidzein-7-O-β-d-glucopyranosyl-(1 → 4)-O-β-d-glucopyranoside (3), and a new natural isoflavone glycoside, daidzein-7-O-α-d-glucopyranosyl-(1 → 4)-O-β-d-glucopyranoside (4) were isolated along with 18 known isoflavones from the EtOAc and n-BuOH fractions of the aqueous extraction of Tongmai granules. All the isoflavones were obtained and determined for the first time from Tongmai granules. The structures of these compounds were elucidated by spectral methods. It was confirmed that the compounds 1-4 were originally from Puerariae Lobatae Radix based on HPLC-DAD analysis of the crude drug extract. The isoflavones isolated were tested for their antioxidative activities by measuring the capacities of scavenging the 2,2'-diphenyl-1-picrylhydrazyl radical.
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University, Beijing, China.
authors.forename: Fu-Rong
authors.initials: FR
authors.lastname: Wang
authors.forename: Xiu-Wei
authors.initials: XW
authors.lastname: Yang
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jian-Xun
authors.initials: JX
authors.lastname: Liu
authors.forename: Xin-Bao
authors.initials: XB
authors.lastname: Yang
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Ren-Bing
authors.initials: RB
authors.lastname: Shi
mesh_terms: D000465:Algorithms; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D001713:Biphenyl Compounds / Q000494:pharmacology; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D007529:Isoflavones / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D019906:Nuclear Magnetic Resonance, Biomolecular; D010851:Picrates / Q000494:pharmacology; D013237:Stereoisomerism
chemical_list: C558553:3'-methoxydaidzein-7,4'-di-O-beta-D-glucopyranoside; D000975:Antioxidants; D001713:Biphenyl Compounds; D004365:Drugs, Chinese Herbal; D006027:Glycosides; D007529:Isoflavones; D010851:Picrates; C558555:biochanin A-8-C-beta-D-apiofuranosyl-(1-6)-O-beta-D-glucopyranoside; C558554:daidzein-7-O-alpha-D-glucopyranosyl-(1-4)-O-beta-D-glucopyranoside; C004931:1,1-diphenyl-2-picrylhydrazyl