title: Field monitoring the seasonal variation in Albatrellus ellisii mycelium abundance with a species-specific genetic marker.
abstract: The conservation of rare fungal sites occurring on actively managed forest lands requires efficient site monitoring methods. In this study species-specific primers for the putatively mycorrhizal Albatrellus ellisii (Russulales) were developed so that DNA extracted from soil samples at known sites of this fungus could be tested for the presence of A. ellisii mycelium with PCR. This method was used to measure seasonal changes in the levels of A. ellisii mycelium at three study sites while the utility of this monitoring method was assessed. We found that A. ellisii maintained a constant level of soil occupancy over three seasons, except at one site where mycelium abundance increased in the fall. No reduction in abundance was seen in the summer, although all three sites experienced significant summer drought. We found species-specific genetic marker detection to be an effective and practical method for monitoring the mycelial distribution of a target fungus in soil. The ability to obtain this data from rare fungal sites advances our capability to conserve these fungi, particularly within the managed forest landscape.
authors.affiliation: Molecular Solutions LLC, Portland, OR 97208, USA. molecularsolutions@msn.com
authors.forename: Matthew
authors.initials: M
authors.lastname: Gordon
authors.forename: Carol
authors.initials: C
authors.lastname: Apple
mesh_terms: D001487:Basidiomycota / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D003247:Conservation of Natural Resources; D017931:DNA Primers; D004271:DNA, Fungal / Q000032:analysis*; D017753:Ecosystem; D005819:Genetic Markers; D025282:Mycelium / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D038821:Mycorrhizae / Q000235:genetics; D009922:Oregon; D016133:Polymerase Chain Reaction; D012621:Seasons; D012988:Soil Microbiology; D013045:Species Specificity; D014197:Trees
chemical_list: D017931:DNA Primers; D004271:DNA, Fungal; D005819:Genetic Markers