title: Interaction between protein 4.1R and spectrin heterodimers.
abstract: Defects or deficiencies in red cell membrane skeletal proteins often undermine the integrity and stability of the plasma membrane, and consequently cause hereditary hemolytic anemias. Genetic and biochemical studies have revealed a complicated picture of the organization of the membrane skeleton, within which α-/β-spectrin heterodimers form a protein lattice. By stabilizing the red cell membrane skeleton, the erythroid protein 4.1R greatly contributes to connecting and regulating the interaction among spectrins, actin filaments and integral proteins on the plasma membrane. In this study, we demonstrated the direct interaction between 4.1R and α-/β-spectrin. The results provide novel insights into the stoichiometry of 4.1R with spectrin, and demonstrate for the first time that the binding ratio of 4.1R to spectrin heterodimers is approximately 5.
authors.affiliation: Weifang Medical College, Wendeng Central Hospital, Weihai, Shandong, People's Republic of China.
authors.forename: Dao-Qiang
authors.initials: DQ
authors.lastname: Zhang
authors.forename: Yun-Peng
authors.initials: YP
authors.lastname: Wang
authors.forename: Wen-Hu
authors.initials: WH
authors.lastname: Wang
authors.forename: Xiu-Mei
authors.initials: XM
authors.lastname: Sui
authors.forename: Jun-Qiang
authors.initials: JQ
authors.lastname: Jiang
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: Jiang
authors.forename: Zu-Shan
authors.initials: ZS
authors.lastname: Xu
authors.forename: Yan-Guang
authors.initials: YG
authors.lastname: Liu
mesh_terms: D002462:Cell Membrane; D003598:Cytoskeletal Proteins / Q000737:chemistry* / Q000378:metabolism; D019281:Dimerization; D004912:Erythrocytes / Q000378:metabolism; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013049:Spectrin / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D003598:Cytoskeletal Proteins; D008565:Membrane Proteins; D011994:Recombinant Proteins; C028841:erythrocyte membrane band 4.1 protein; D013049:Spectrin