title: MsbA ATP-binding cassette (ABC) transporter of E. coli: structure and possible flippase mechanism.
abstract: ATP-binding cassette (ABC) transporters utilize the energy present in cellular ATP to drive the translocation of structurally diverse set of solutes across the membrane barriers of eubacteria, archaebacteria and eukaryotes. In bacteria, these transporters are considered to be important virulence factors because they play role in nutrient uptake and in the secretion of toxins. The advances in structural determination and functional analysis of bacterial transporters have greatly increased our understanding of the mechanism of transport of these ABC transporters. Although progress in the field of structural biology has been made with the prokaryotic family members, it is likely that eukaryotic transporters will utilize the same mechanisms for translocation process. In this review, we summarize the function of the known MsbA ABC transporters in E. coli and mechanistic insights from structural and possible flippase mechanism studies.
authors.affiliation: N. T. Lab-I, Department Biochemistry, National Dairy Research Institute, Karnal, India. gkndri@gmail.com
authors.forename: Gautam
authors.initials: G
authors.lastname: Kaul
authors.forename: Gurulingappa
authors.initials: G
authors.lastname: Pattan
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000737:chemistry* / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000737:chemistry; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D001692:Biological Transport / Q000502:physiology; D019281:Dimerization; D004926:Escherichia coli / Q000378:metabolism*; D006868:Hydrolysis; D026901:Membrane Transport Proteins / Q000737:chemistry* / Q000378:metabolism*; D049294:Phospholipid Transfer Proteins / Q000737:chemistry* / Q000378:metabolism; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary
chemical_list: D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D026901:Membrane Transport Proteins; C079726:MsbA protein, Bacteria; D049294:Phospholipid Transfer Proteins; D000255:Adenosine Triphosphate