title: Detection of isoform-specific fibroblast growth factor receptors by whole-mount in situ hybridization in early chick embryos.
abstract: We have developed "b" and "c" isoform-specific chicken fibroblast growth factor (FGF) receptor 1-3 probes for in situ hybridization. We rigorously demonstrate the specificity of these probes by using both dot blot hybridization and whole-mount in situ hybridization during neurulation and early postneurulation stages, and we compare expression patterns of each of the three isoform-specific probes to one another and to generic probes to each of the three (non-isoform-specific) FGF receptors. We show that the expression pattern of each receptor is represented by the collective expression of each of its two isoforms, with the expression of each FGF receptor being most similar to that of its "c" isoform at two of the three stages studied, and that tissue and stage differences exist in the patterns of expression of the six isoforms. We demonstrate the usefulness of these probes for defining the differential tissue expression of FGF receptor 1-3 isoforms.
authors.affiliation: Department of Neurobiology and Anatomy, University of Utah School of Medicine, Salt Lake City, Utah 84132-3401, USA.
authors.forename: Junko
authors.initials: J
authors.lastname: Nishita
authors.forename: Sho
authors.initials: S
authors.lastname: Ohta
authors.forename: Steven B
authors.initials: SB
authors.lastname: Bleyl
authors.forename: Gary C
authors.initials: GC
authors.lastname: Schoenwolf
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D002642:Chick Embryo / Q000378:metabolism; D002645:Chickens / Q000235:genetics / Q000378:metabolism*; D047108:Embryonic Development / Q000235:genetics / Q000502:physiology; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization / Q000379:methods*; D008954:Models, Biological; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000235:genetics; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D017468:Receptors, Fibroblast Growth Factor / Q000235:genetics / Q000378:metabolism*; D017385:Sequence Homology; D013997:Time Factors; D014018:Tissue Distribution
chemical_list: D020033:Protein Isoforms; D017468:Receptors, Fibroblast Growth Factor