title: Discordance at D3S1358 locus involving SGM Plus™ and the European new generation multiplex kits.
abstract: During the course of routine database sample analysis in the Israel Police DNA database, an off-ladder D3S1358 allele, calculated to be >22.1, extending into the adjacent vWA locus was observed using Applied Biosystems SGM Plus™ kit. To verify the size of this D3S1358 long allele and to ensure it was not part of a trialle pattern in the neighboring locus, the sample was amplified using three of the European new generation STR multiplex kits: NGM(TM) (Applied Biosystem), Powerplex™ ESX and ESI (Promega). The results of these amplifications determined the variant to be a 22 allele. Subsequent sequencing confirmed this designation and revealed a nucleotide polymorphism. Ten additional SGM Plus™ profiled samples with D3S1358 alleles larger than 19, were re-analyzed using NGM(TM) and Powerplex™ ESX which also showed discordance in the calculated results between original SGM Plus™ designations and those obtained with the European new generation multiplexes.
authors.affiliation: DNA Database Laboratory, Division of Identification and Forensic Science (DIFS), Israel Police, National H.Q., Jerusalem 91906, Israel.
authors.forename: Aliza
authors.initials: A
authors.lastname: Raziel
authors.forename: Carla
authors.initials: C
authors.lastname: Oz
authors.forename: Aviva Dell'Ariccia
authors.initials: AD
authors.lastname: Carmon
authors.forename: Rafi
authors.initials: R
authors.lastname: Ilsar
authors.forename: Ashira
authors.initials: A
authors.lastname: Zamir
mesh_terms: D000483:Alleles; D001483:Base Sequence; D002874:Chromosome Mapping; D017931:DNA Primers; D030541:Databases, Genetic; D005060:Europe; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D017931:DNA Primers