title: Up-regulation of the G3PDH 'housekeeping' gene by estrogen.
abstract: Proteomic and genomic studies commonly involve the assessment of mRNA levels using reverse transcription-polymerase chain reaction (PCR) and real-time quantitative PCR. An internal standard RNA is fundamentally analyzed along with the investigated mRNA to document the specificity of the effect(s) on mRNA and to correct for inter-sample variations. In our studies implementing estrogen treatments on different cell lines, we initially used glyceraldehyde-3-phosphate dehydrogenase (G3PDH) as an internal standard. However, the results of PCR amplification demonstrated that 17β-estradiol enhanced the expression of the G3PDH gene, rendering it impossible to use G3PDH as an unbiased comparative control.
authors.affiliation: Departments of Oral and Maxillofacial Surgery, and Molecular Cell Pharmacology, Hokkaido University, Graduate School of Dental Medicine, Sapporo, Hokkaido, Japan.
authors.forename: Nadia
authors.initials: N
authors.lastname: Galal
authors.forename: Waleed
authors.initials: W
authors.lastname: El-Beialy
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Deyama
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Yoshimura
authors.forename: Kanchu
authors.initials: K
authors.lastname: Tei
authors.forename: Kuniaki
authors.initials: K
authors.lastname: Suzuki
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Totsuka