title: Paridis saponins inhibiting carcinoma growth and metastasis in vitro and in vivo.
abstract: Paris polyphylla Smith var. yunnanensis extracts, Rhizoma Paridis saponins (RPS) have been found to show strong antitumor activity. However, few studies have yet investigated pulmonary metastasis treatment with this herb. To detail the effective components in RPS and discuss the preliminary mechanism of antitumor effects in vivo and in vitro, a mixture isolated from RPS was investigated. The main constituents were identified as polyphyllin D, formosanin C, dioscin, Paris H, Paris VII and pennogennin 3-O-α-L-rhamnopyranosyl (1→4)-[β-L-rhamnopyranosyl (1→2)]-β-D-glucopyranoside. In our experiments, LA795 cells were exposed to the mixed compounds. Migration inhibition was evaluated by wound healing assay and migration assay in non-cytotoxic dose which was determined by MTT assay. The results demonstrated that the constituent in varying degrees inhibited the migration of the tumor cells in vitro. The mixture also showed antitumor effects on carcinoma in vivo. In conclusion, the mixture is a potent anticancer agent that elicits programmed cell death and inhibits the migration in murine lung adenocarcinoma, both in vitro and in vivo.
authors.affiliation: School of Pharmaceutical Science and Technology, Tianjin University, China.
authors.forename: Man
authors.initials: M
authors.lastname: Shuli
authors.forename: Gao
authors.initials: G
authors.lastname: Wenyuan
authors.forename: Zhang
authors.initials: Z
authors.lastname: Yanjun
authors.forename: Ma
authors.initials: M
authors.lastname: Chaoyi
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Li
authors.initials: L
authors.lastname: Yiwen
mesh_terms: D000230:Adenocarcinoma / Q000188:drug therapy / Q000473:pathology; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D005260:Female; D019668:Liliaceae / Q000737:chemistry*; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D027343:Rhizome; D012503:Saponins / Q000302:isolation & purification / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D012503:Saponins