title: A comparative study of thermal calcination and an alkaline hydrolysis method in the isolation of hydroxyapatite from Thunnus obesus bone.
abstract: In the present study, hydroxyapatite (HAp) was isolated from Thunnus obesus bone using alkaline hydrolysis and thermal calcination methods. The obtained ceramic has been characterized by thermal gravimetric analysis (TGA), Fourier transform infrared spectroscopy (FT-IR), powder x-ray diffraction analysis (XRD), field-emission scanning electron microscopy, energy-dispersive x-ray analysis, transmission electron microscopy (TEM), selected area diffraction analysis, cytotoxic analysis and cell proliferation analysis. The results indicate that there are significant differences between the ceramics and T. obesus bone. FT-IR and TGA results affirmed that the collagen and organic moieties have been eliminated by both the proposed methods. XRD results were in agreement with JCPDS data. TEM and selective area diffraction images have signified that the thermal calcination method produces good crystallinity with dimensions 0.3-1.0 µm, whereas the alkaline hydrolysis method produces nanostructured HAp crystals with 17-71 nm length and 5-10 nm width. Biocompatibility of HAp crystals was evaluated by cytotoxicity and cell proliferation with human osteoblast-like cell MG-63.
authors.affiliation: Department of Chemistry, Pukyong National University, Busan 608-737, Korea.
authors.forename: Jayachandran
authors.initials: J
authors.lastname: Venkatesan
authors.forename: Zhong Ji
authors.initials: ZJ
authors.lastname: Qian
authors.forename: BoMi
authors.initials: B
authors.lastname: Ryu
authors.forename: Noel Vinay
authors.initials: NV
authors.lastname: Thomas
authors.forename: Se Kwon
authors.initials: SK
authors.lastname: Kim
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D001842:Bone and Bones / Q000378:metabolism*; D002113:Calcification, Physiologic; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002516:Ceramics; D003460:Crystallization; D004305:Dose-Response Relationship, Drug; D017886:Durapatite / Q000737:chemistry* / Q000302:isolation & purification; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D046529:Microscopy, Electron, Transmission / Q000379:methods; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D013818:Thermogravimetry / Q000379:methods; D014413:Tuna; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D017886:Durapatite