title: [Determination of liquiritin, naringin, hesperidin and glycyrrhizic acid in extractive of Wendan formula by RP-HPLC].
abstract: OBJECTIVE
  To develop a RP-HPLC method for simultaneous determination of liquiritin, naringin, hesperidin and glycyrrhizic acid in extraction of Wendan formula.
  
  
  METHODS
  DIKMA Diamonsil(2)-C18 column (4.6 mm x 250 mm, 5 microm) was used at 25 degrees C with the mobile phase of acetonitrile-0.1% phosphatic acid in a gradient manner. The flow rate was set at 1.0 mL min(-1). The detection wavelength was 237, 283 nm.
  
  
  RESULTS
  The linear responses ranged from 0.0199-0.1191 microg for liquiritin (r = 0.9997, n = 6), 0.1800-1.0800 microg for naringin (r = 0.9997, n = 5), 0.1455-0.8730 microg for hesperidin (r = 0.9998, n = 6), 0.0393-0.2355 microg for monoammonium glycyrrhizinate (r = 0.9997, n = 6), respectively. The average recoveries were 97.7% with RSD 1.5% for liquiritin, 97.7% with RSD 2.0% for naringin, 97.1% with RSD 2.0% for hesperidin and 98.5% with RSD 1.9% for glycyrrhizic acid, respectively.
  
  
  CONCLUSIONS
  The method is quick, simple and repeatable for simultaneous determination of liquiritin, naringin, hesperidin and glycyrrhizic acid in extraction of Wendan formula.
authors.affiliation: Science and Technology Innovation of Small and Mid-sized Enterprise Fund Management Center, Ministry of Science and Technology, Beijing 10038, China. ywzhou@pku.edu.cn
authors.forename: Dongming
authors.initials: D
authors.lastname: Xu
authors.forename: Kejian
authors.initials: K
authors.lastname: Cheng
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000302:isolation & purification; D044950:Flavanones / Q000032:analysis* / Q000302:isolation & purification; D005960:Glucosides / Q000032:analysis* / Q000302:isolation & purification; D019695:Glycyrrhizic Acid / Q000032:analysis* / Q000302:isolation & purification; D006569:Hesperidin / Q000032:analysis* / Q000302:isolation & purification
chemical_list: D004365:Drugs, Chinese Herbal; D044950:Flavanones; D005960:Glucosides; C528423:wendan; D019695:Glycyrrhizic Acid; D006569:Hesperidin; C005274:naringin; C512196:liquiritin