title: Complete nucleotide sequence of a begomovirus and associated betasatellite infecting croton (Croton bonplandianus) in Pakistan.
abstract: The complete sequences of a begomovirus and an associated betasatellite isolated from Croton bonplandianus originating from Pakistan were determined. The sequence of the begomovirus showed the highest level of nucleotide sequence identity (88.9%) to an isolate of papaya leaf curl virus and thus represents a new species, for which we propose the name Croton yellow vein virus (CYVV). The sequence of the betasatellite showed the highest levels of sequence identity (82 to 98.4%) to six sequences in the databases that have yet to be reported, followed by isolates of tomato leaf curl Joydebpur betasatellite (48.7 to 52.5%). This indicates that the betasatellite identified here (and the six sequences in the databases) is an isolate of a newly identified species for which the name Croton yellow vein mosaic betasatellite (CroYVMB) is proposed. For the begomovirus, an analysis of the sequence indicates that it has a recombinant origin.
authors.affiliation: Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering, Jhang Road, Faisalabad, Pakistan.
authors.forename: Khadim
authors.initials: K
authors.lastname: Hussain
authors.forename: Mazhar
authors.initials: M
authors.lastname: Hussain
authors.forename: Shahid
authors.initials: S
authors.lastname: Mansoor
authors.forename: Rob W
authors.initials: RW
authors.lastname: Briddon
mesh_terms: D001483:Base Sequence; D053839:Begomovirus / Q000235:genetics* / Q000302:isolation & purification*; D029802:Croton / Q000821:virology*; D004279:DNA, Viral / Q000235:genetics; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010154:Pakistan; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D016133:Polymerase Chain Reaction; D012526:Satellite Viruses / Q000235:genetics* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004279:DNA, Viral