title: Effect of heat challenge on peripheral blood mononuclear cell viability: comparison of a tropical and temperate pig breed.
abstract: We evaluated the effect of heat challenge on cell viability, concanavalin A-induced proliferation and heat shock protein (HSPs) mRNA expression in peripheral mononuclear blood cells (PBMC) isolated from Creole (CR) and Large White (LW) pigs. The PBMCs were cultured for 9 h at 37 °C before being subjected to heat challenge: (1) at 42 °C or 45 °C for 2, 4, 6 and 9 h to monitor cell viability;(2) at 45 °C for 2 and 9 h followed by stimulation for 24 h at 37 °C with concanavalin A to evaluate mitogen-induced proliferation; and (3) at 45 °C for 3, 6 and 9 h to measure induction of HSP70.2 and HSP90 mRNA. Cell viability was affected by breed and temperature (P < 0.01), and the viability decrease caused by heat challenge was greater for LW than CR pigs. For mitogen-stimulated PBMCs, incubation at 45 °C reduced lymphoblastogenesis equally in both breeds (P < 0.01). Although heat challenge for 3 and 6 h at 45°C induced expression of HSP70.2 and HSP90 mRNA, no breed difference was observed. In conclusion, differences in heat resistance between these two breeds at the whole organism level are reflected at the cellular level. Neither HSP70.2 nor HSP90 mRNA expression levels explain this effect.
authors.affiliation: INRA UR143, Unité de Recherches Zootechniques, Centre INRA Antilles Guyane, Domaine de Duclos, Prise d'Eau, 97170 Petit Bourg, Guadeloupe, French West Indies, France. jean-christophe.bambou@antilles.inra.fr
authors.forename: Jean-Christophe
authors.initials: JC
authors.lastname: Bambou
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Gourdine
authors.forename: Roxanne
authors.initials: R
authors.lastname: Grondin
authors.forename: Nathalie
authors.initials: N
authors.lastname: Vachiery
authors.forename: David
authors.initials: D
authors.lastname: Renaudeau
mesh_terms: D000818:Animals; D002470:Cell Survival; D003208:Concanavalin A / Q000494:pharmacology; D019430:Guadeloupe; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D018841:HSP90 Heat-Shock Proteins / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D018869:Heat-Shock Response; D006358:Hot Temperature / Q000009:adverse effects*; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008213:Lymphocyte Activation; D008297:Male; D012333:RNA, Messenger / Q000097:blood / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D013045:Species Specificity; D034421:Sus scrofa / Q000502:physiology*; D013997:Time Factors; D014329:Tropical Climate
chemical_list: D018840:HSP70 Heat-Shock Proteins; D018841:HSP90 Heat-Shock Proteins; D012333:RNA, Messenger; D003208:Concanavalin A