title: The distribution of APGWamide and RFamides in the central nervous system and ovary of the giant freshwater prawn, Macrobrachium rosenbergii.
abstract: Immunohistochemistry was used to identify the distribution of both APGWamide-like and RFamide-like peptides in the central nervous system (CNS) and ovary of the mature female giant freshwater prawn, Macrobrachium rosenbergii. APGWamide-like immunoreactivity (ALP-ir) was found only within the sinus gland (SG) of the eyestalk, in small- and medium-sized neurons of cluster 4, as well as their varicosed axons. RFamide-like immunoreactivity (RF-ir) was detected in neurons of all neuronal clusters of the eyestalk and CNS, except clusters 1 and 5 of the eyestalk, and dorsal clusters of the subesophageal, thoracic, and abdominal ganglia. The RF-ir was also found in all neuropils of the CNS and SG, except the lamina ganglionaris. These immunohistochemical locations of the APGWamide-like and RF-like peptides in the eyestalk indicate that these neuropeptides could modulate the release of the neurohormones in the sinus gland. The presence of RFamide-like peptides in the thoracic and abdominal ganglia suggests that it may act as a neurotransmitter which controls muscular contractions. In the ovary, RF-ir was found predominantly in late previtellogenic and early vitellogenic oocytes, and to a lesser degree in late vitellogenic oocytes. These RFs may be involved with oocyte development, but may also act with other neurohormones and/or neurotransmitters within the oocyte in an autocrine or paracrine manner.
authors.affiliation: Department of Anatomy, Mahidol University, Bangkok, Thailand.
authors.forename: Ronnarong
authors.initials: R
authors.lastname: Palasoon
authors.forename: Sasiporn
authors.initials: S
authors.lastname: Panasophonkul
authors.forename: Prapee
authors.initials: P
authors.lastname: Sretarugsa
authors.forename: Peter
authors.initials: P
authors.lastname: Hanna
authors.forename: Prasert
authors.initials: P
authors.lastname: Sobhon
authors.forename: Jittipan
authors.initials: J
authors.lastname: Chavadej
mesh_terms: D000818:Animals; D002490:Central Nervous System / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D005260:Female; D007150:Immunohistochemistry; D018613:Microscopy, Confocal; D009474:Neurons / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D009479:Neuropeptides / Q000032:analysis / Q000378:metabolism*; D009865:Oocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D010053:Ovary / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D033541:Palaemonidae / Q000033:anatomy & histology / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D009479:Neuropeptides; C063193:alanyl-prolyl-glycyl-tryptophanamide; C058047:arginylphenylalaninamide