title: Synthesis and transfection activity of novel galactosylated polycationic lipid.
abstract: In this study, we synthesized a new galactosylated cationic lipid and investigated its biological activity. The structure of lipid combines both spermine residue for DNA compaction and galactose moiety for the improvement of aggregation behavior of lipoplexes. Lipid was low toxic for different mammalian cells, and was able both to compact plasmid DNA and to mediate cellular accumulation of various nucleic acids (ODN, pDNA and siRNA) exhibiting biological activity (transgene expression, gene silencing).
authors.affiliation: Lomonosov Moscow State Academy of Fine Chemical Technology (MITHT), 86 Vernadskogo Ave., Moscow 119571, Russian Federation. mamaslov@mail.ru
authors.forename: M A
authors.initials: MA
authors.lastname: Maslov
authors.forename: D A
authors.initials: DA
authors.lastname: Medvedeva
authors.forename: D A
authors.initials: DA
authors.lastname: Rapoport
authors.forename: R N
authors.initials: RN
authors.lastname: Serikov
authors.forename: N G
authors.initials: NG
authors.lastname: Morozova
authors.forename: G A
authors.initials: GA
authors.lastname: Serebrennikova
authors.forename: V V
authors.initials: VV
authors.lastname: Vlassov
authors.forename: M A
authors.initials: MA
authors.lastname: Zenkova
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006224:Cricetinae; D005434:Flow Cytometry; D005690:Galactose / Q000737:chemistry*; D018014:Gene Transfer Techniques; D006801:Humans; D008055:Lipids / Q000138:chemical synthesis* / Q000737:chemistry / Q000633:toxicity; D008081:Liposomes / Q000737:chemistry; D018625:Microscopy, Atomic Force; D015394:Molecular Structure; D011073:Polyamines / Q000737:chemistry*; D000071228:Polyelectrolytes; D014162:Transfection
chemical_list: D005690:Galactose; D008055:Lipids; D008081:Liposomes; D011073:Polyamines; D000071228:Polyelectrolytes