title: FRET detection of proteins using fluorescently doped electrospun nanofibers and pattern recognition.
abstract: This paper reports the fabrication of solid-state nanofiber sensor arrays and their use for detection of multiple proteins using principal component analysis (PCA). Four cationic and anionic fluorescently embedded nanofibers are generated by an electrospinning method, yielding unique patterns of fluorescence change upon interaction with protein samples. Five metal and nonmetal containing proteins, i.e., hemoglobin, myoglobin, cytochrome c, BSA, and avidin, have been investigated; and the results show that distinct fluorescent patterns can be formed upon the addition of protein samples to the array of solid nanofiber substrates, allowing their unambiguous identification. The nanofiber films are highly repeatable with a batch-to-batch variation of approximately 5% and demonstrated outstanding reusability with less than a 15% loss of fluorescence intensity signal after 5 regenerations of test cycles. For a more practical visualization, a cluster map was generated using PCA of the change-in-fluorescence (ΔI) composite patterns, demonstrating the potential of the method for diagnostic applications.
authors.affiliation: Department of Chemistry, University of California, Riverside, California 92521, United States.
authors.forename: Bryce W
authors.initials: BW
authors.lastname: Davis
authors.forename: Nakorn
authors.initials: N
authors.lastname: Niamnont
authors.forename: Robert
authors.initials: R
authors.lastname: Dillon
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Bardeen
authors.forename: Mongkol
authors.initials: M
authors.lastname: Sukwattanasinitt
authors.forename: Quan
authors.initials: Q
authors.lastname: Cheng
mesh_terms: D000114:Acetylene / Q000031:analogs & derivatives / Q000737:chemistry; D000818:Animals; D002417:Cattle; D050091:Dendrimers / Q000737:chemistry; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry*; D057139:Nanofibers / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D055620:Optical Phenomena; D010363:Pattern Recognition, Automated / Q000379:methods*; D010449:Peptide Mapping; D025341:Principal Component Analysis; D011506:Proteins / Q000032:analysis* / Q000737:chemistry*; D012996:Solutions
chemical_list: D050091:Dendrimers; D005456:Fluorescent Dyes; D011506:Proteins; D012996:Solutions; C044735:biphenylacetylene; D000114:Acetylene