title: PARP-1 activity in normal and cancerous human endometrium and its relationship with quantity of abasic sites (AP).
abstract: OBJECTIVE
  Poly (ADP-ribose) polymerase (PARP-1) is involved in the processes of DNA repair contributing to the maintenance of genomic stability. Recent data suggest that polymerase is involved in the development of endometrial adenocarcinomas and more advanced tumors displaying lowest enzyme protein expression. Data on PARP-1 activity regarding carcinogenesis in human endometrium are scarce. That was the reason why the authors of the present work wished to investigate the enzyme activity in human uterine hormone-dependent cancer and to compare the results with those obtained for normal endometrial tissue. The next aim was to check whether enzyme activity in normal and cancerous endometrium depends on the number of AP sites, which are widely known as oxidative stress DNA damage markers and PARP-1 activity stimulators.
  
  
  METHODS
  Universal Colorimetric PARP Assay Kit was used to estimate the enzyme activity in units/ mg protein. Apurinic sites/105 base pairs (bp) were measured by Oxidative DNA Damage Kit Quantitative. Results were calculated for 47 endometrial samples and 15 uterine adenocarcinomas specimens. Finally the PARP-1 activity was analyzed for histological and some clinical features of neoplasms.
  
  
  CONCLUSIONS
  1. no differences in PARP-1 activity were found in non-cancerous types of human endometrium; 2. mean enzyme activity was lower in sporadic endometrial cancers than in noncancerous endometrial specimens (2.89 +/- 0.55 vs 6.39 +/- 0.06; p < 0.005); 3. mean PARP-1 activity in lower grade neoplasms was higher than in G3 tumors and was lower in adenocarcinomas displaying deep uterine wall infiltration; 4. there was no relationship between PARP-1 activity and AP level.
authors.affiliation: 2nd Department of Gynecology, Medical University of Lublin, Lublin, Jaczewskiego 8, Poland. postawski@yahoo.com
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Postawski
authors.forename: Marta
authors.initials: M
authors.lastname: Monist
authors.forename: Gérard
authors.initials: G
authors.lastname: Keith
mesh_terms: D000231:Adenocarcinoma, Papillary / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D004249:DNA Damage; D016889:Endometrial Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D004717:Endometrium / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D009361:Neoplasm Invasiveness / Q000235:genetics; D009367:Neoplasm Staging; D009376:Neoplasms, Hormone-Dependent / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D018384:Oxidative Stress; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: C499320:PARP1 protein, human; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases