title: Immobilization of human carbonic anhydrase on gold nanoparticles assembled onto amine/thiol-functionalized mesoporous SBA-15 for biomimetic sequestration of CO2.
abstract: A biocatalyst was synthesized by immobilizing human carbonic anhydrase onto gold nanoparticles assembled over amine/thiol-functionalized mesoporous SBA-15. The physicochemical properties of the functionalized mesoporous SBA-15 were obtained by XRD, BET, FE SEM, HR TEM, EDS, and zeta potential analysis. The biocatalytic performance was studied for para-nitrophenyl acetate (p-NPA) hydrolysis. The kinetic parameters K(m) were found to be 22.35 and 27.75 mM, and K(cat)/K(m) values were 1514.09 and 1612.25 M(-1) s(-1) for HCA immobilized on gold nanoparticles assembled on amine/thiol-functionalized mesoporous SBA-15 (HCA/Au/APTES/SBA-15 and HCA/Au/MPTES/SBA-15), respectively. These HCA/Au/APTES/SBA-15 and HCA/Au/MPTES/SBA-15 were investigated for biocatalytic hydration of CO(2) and its precipitation as CaCO(3). The amount of CaCO(3) precipitated over HCA/Au/MPTES/SBA-15 was nearly the same as that precipitated over free HCA. Storage stability and reusability studies suggested that HCA/Au/MPTES/SBA-15 retained its activity even after 20 days storage at 25 °C and 20 recycling runs. The present results demonstrate that HCA/Au/MPTES/SBA-15 and HCA/Au/APTES/SBA-15 are highly efficient potential nanobiocatalysts for industrial-scale CO(2) sequestration.
authors.affiliation: Korea Institute of Energy Research, Daejeon 305-343, Korea.
authors.forename: Mari
authors.initials: M
authors.lastname: Vinoba
authors.forename: Kyoung Soo
authors.initials: KS
authors.lastname: Lim
authors.forename: Si Hyun
authors.initials: SH
authors.lastname: Lee
authors.forename: Soon Kwan
authors.initials: SK
authors.lastname: Jeong
authors.forename: Muthukaruppan
authors.initials: M
authors.lastname: Alagar
mesh_terms: D000588:Amines / Q000737:chemistry; D055162:Biocatalysis; D032701:Biomimetics / Q000379:methods*; D002245:Carbon Dioxide / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D002256:Carbonic Anhydrases / Q000737:chemistry* / Q000378:metabolism; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D006801:Humans; D006868:Hydrolysis; D053768:Metal Nanoparticles / Q000737:chemistry*; D010648:Phenylacetates / Q000737:chemistry; D016062:Porosity; D011437:Propylamines; D012821:Silanes / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry
chemical_list: D000588:Amines; D004800:Enzymes, Immobilized; D010648:Phenylacetates; D011437:Propylamines; C509969:SBA-15; D012821:Silanes; D013438:Sulfhydryl Compounds; D002245:Carbon Dioxide; D006046:Gold; D012822:Silicon Dioxide; D002256:Carbonic Anhydrases; C025136:phenylacetic acid; C477625:amino-propyl-triethoxysilane