title: Heat-shock protein HSPA4 is required for progression of spermatogenesis.
abstract: Heat-shock protein 110 (HSP110) family members act as nucleotide exchange factors (NEF) of mammalian and yeast HSP70 chaperones during the ATP hydrolysis cycle. In this study, we describe the expression pattern of murine HSPA4, a member of the HSP110 family, during testis development and the consequence of HSPA4 deficiency on male fertility. HSPA4 is ubiquitously expressed in all the examined tissues. During prenatal and postnatal development of gonad, HSPA4 is expressed in both somatic and germ cells; however, expression was much higher in germ cells of prenatal gonads. Analyses of Hspa4-deficient mice revealed that all homozygous mice on the hybrid C57BL/6J×129/Sv genetic background were apparently healthy. Although HSPA4 is expressed as early as E13.5 in male gonad, a lack of histological differences between Hspa4(-/-) and control littermates suggests that Hspa4 deficiency does not impair the gonocytes or their development to spermatogonia. Remarkably, an increased number of the Hspa4-deficient males displayed impaired fertility, whereas females were fertile. The total number of spermatozoa and their motility were drastically reduced in infertile Hspa4-deficient mice compared with wild-type littermates. The majority of pachytene spermatocytes in the juvenile Hspa4(-/-) mice failed to complete the first meiotic prophase and became apoptotic. Furthermore, down-regulation of transcription levels of genes known to be expressed in spermatocytes at late stages of prophase I and post-meiotic spermatids leads to suggest that the development of most spermatogenic cells is arrested at late stages of meiotic prophase I. These results provide evidence that HSPA4 is required for normal spermatogenesis.
authors.affiliation: Institute of Human Genetics, University of Göttingen, Göttingen, Germany.
authors.forename: Torsten
authors.initials: T
authors.lastname: Held
authors.forename: Amal Z
authors.initials: AZ
authors.lastname: Barakat
authors.forename: Belal A
authors.initials: BA
authors.lastname: Mohamed
authors.forename: Ilona
authors.initials: I
authors.lastname: Paprotta
authors.forename: Andreas
authors.initials: A
authors.lastname: Meinhardt
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Engel
authors.forename: Ibrahim M
authors.initials: IM
authors.lastname: Adham
mesh_terms: D000818:Animals; D017209:Apoptosis; D004622:Embryo, Mammalian / Q000378:metabolism / Q000473:pathology; D047108:Embryonic Development; D005260:Female; D005298:Fertility; D050957:HSP110 Heat-Shock Proteins / Q000235:genetics / Q000502:physiology*; D006720:Homozygote; D007248:Infertility, Male / Q000196:embryology / Q000378:metabolism* / Q000473:pathology; D008297:Male; D049469:Meiotic Prophase I; D051379:Mice; D057507:Mice, 129 Strain; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009928:Organ Specificity; D012333:RNA, Messenger / Q000378:metabolism; D055101:Semen Analysis; D013081:Sperm Motility; D013091:Spermatogenesis; D013094:Spermatozoa / Q000378:metabolism* / Q000473:pathology; D013737:Testis / Q000196:embryology / Q000254:growth & development / Q000378:metabolism / Q000473:pathology
chemical_list: D050957:HSP110 Heat-Shock Proteins; C485680:Hspa4 protein, mouse; D012333:RNA, Messenger