title: Development of a highly selective fluorescence probe for alkaline phosphatase.
abstract: We have developed the first highly selective fluorescence probe for alkaline phosphatase (ALP), TG-mPhos. This probe shows selectivity for ALP over protein tyrosine phosphatase and protein serine/threonine phosphatase. Our previously developed TG-Phos, which has a phenolic phosphate linkage in place of the alcoholic phosphate linkage of TG-mPhos, lacks this selectivity. TG-mPhos should enable precise fluorescence imaging of ALP activity in biological applications.
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Mitsuyasu
authors.initials: M
authors.lastname: Kawaguchi
authors.forename: Kenjiro
authors.initials: K
authors.lastname: Hanaoka
authors.forename: Toru
authors.initials: T
authors.lastname: Komatsu
authors.forename: Takuya
authors.initials: T
authors.lastname: Terai
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Nagano
mesh_terms: D000469:Alkaline Phosphatase / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D010777:Photochemistry; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes; D000469:Alkaline Phosphatase