title: The fluorescence theatre: a cost-effective device using theatre gels for fluorescent protein and dye screening.
abstract: Here we report a simple cost-effective device for screening colonies on plates for expression of the monomeric red fluorescent protein mRFP1 and the fluorescent dye Nile red. This device can be built from any simple light source, in our case a Quebec Colony Counter, and cost-effective theatre gels. The device can be assembled in as little as 20 min, and it produces excellent results when screening a large number of colonies.
authors.affiliation: Department of Biology, University of Waterloo, ON, Canada.
authors.forename: John R
authors.initials: JR
authors.lastname: Heil
authors.forename: Ricardo F
authors.initials: RF
authors.lastname: Nordeste
authors.forename: Trevor C
authors.initials: TC
authors.lastname: Charles
mesh_terms: D005453:Fluorescence; D005456:Fluorescent Dyes / Q000032:analysis* / Q000378:metabolism; D005782:Gels; D008164:Luminescent Proteins / Q000032:analysis / Q000378:metabolism; D008828:Microbiological Techniques / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D000097573:Red Fluorescent Protein
chemical_list: D005456:Fluorescent Dyes; D005782:Gels; D008164:Luminescent Proteins; D000097573:Red Fluorescent Protein