title: [Study on immobilization of heparin on surface of Ti-O films and its antithrombogenicity].
abstract: Photoreactive heparin was synthesized by reaction of 4-azidoaniline and heparin. An organic layer was introduced on the surface of Ti-O by 3-aminopropylphosphonic acid assembling, and then the modified heparin was immobilized on the surface by UV irradiation. Water contact angle was used to characterize the hydrophilicity, quantitive assay was done by azure staining methods, and blood compatibility was evaluated by platelet adhesion experiment. Water contact angle of heparinized surface was smaller than that of Ti-O film, which indicated more hydrophilic property of heparinized surface. The surface density of heparin increased with the prolonging of irradiation time and the density was 2.1 microg/cm2 when irradiated for 300s. It showed the heparinized surface was effective in resisting platelets from adhesion and aggregation.
authors.affiliation: Key Lab for Advanced Technologies of Materials of Ministry of Education, Southwest Jiaotong University, Key Lab of Surface Engineering of Artificial Organs of Sichuan, Chengdu 610031, China.
authors.forename: Yajun
authors.initials: Y
authors.lastname: Weng
authors.forename: Hongmei
authors.initials: H
authors.lastname: Tan
authors.forename: Nan
authors.initials: N
authors.lastname: Huang
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.forename: Junying
authors.initials: J
authors.lastname: Chen
authors.forename: Yongxiang
authors.initials: Y
authors.lastname: Leng
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry; D001391:Azo Compounds / Q000737:chemistry; D001798:Blood Proteins / Q000494:pharmacology; D005343:Fibrinolytic Agents / Q000494:pharmacology*; D006493:Heparin / Q000737:chemistry*; D006801:Humans; D008567:Membranes, Artificial; D011437:Propylamines / Q000737:chemistry; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D000814:Aniline Compounds; D001391:Azo Compounds; D001798:Blood Proteins; D005343:Fibrinolytic Agents; D008567:Membranes, Artificial; D011437:Propylamines; C024875:platelet adhesion inhibitor; C070827:4-azidoaniline; C009495:titanium dioxide; D006493:Heparin; D014025:Titanium; C011649:3-aminopropylphosphonic acid