title: [Effect of weight reduction programme on C-peptide concentration and lipid profile in obese children aged 4 to 10 years].
abstract: BACKGROUND
  Early childhood obesity may play an important role in development of the metabolic syndrome characterized by abnormalities in several major cardiovascular risk factors. Only a few studies demonstrate that weight reduction leads to an improvement of the atherogenic risk-factor profile in prepubertal children.
  
  
  OBJECTIVE
  The aim of this study was to assess the concentration of C-peptide and selected parameters of lipid profile in prepubertal obese children before and after weight reduction therapy.
  
  
  METHODS
  Changes in nutrition, anthropometric and biochemical parameters including C-peptide concentration and lipid profile were determined in 52 obese children (z-score BMI > or =2SD) aged 4-10 years, before and after a 3-months lifestyle intervention programme. This programme consisted of dietary (1200-1400 kcal/day) and physical activity modifications and behaviour therapy, including individual psychological care of the child and its family. The reference group consisted of 30 healthy normal-weight children. Total cholesterol, LDL and HDL-cholesterol and triglicerydes levels were measured by routine enzymatic methods. Concentration of C-peptide was determined using the immunoenzymatic kit.
  
  
  RESULTS
  All obese children were divided into two groups: A and B. In group A with decreased BMI by 10% (25.4 +/- 3.1 vs. 22.8 +/- 2.9 kg/mi; p < 0.01) after the 3-months therapy we found also decreased concentrations of triglicerydes and C-peptide by about 20% (77.9 +/- 28.5 vs. 60.7 +/- 17.5 mg/dl; p < 0.02) and 35% (2.96 +/- 1.52 vs. 1.94 +/- 1.40 ng/ml; p < 0.05) respectively. In group B, without changes in the BMI during therapy, no significant differences in these biochemical parameters were observed. Moreover, higher level of total cholesterol and LDL-cholesterol by 10% (p < 0.05) observed in this group may be related to dietary cholesterol, which was similar both at baseline and 3 months later.
  
  
  CONCLUSIONS
  We suggest that weight-reduction programme is necessary in prepubertal obese children with abnormal lipid profile as a prevention of metabolic syndrome in later life. Our findings support the beneficial effects of weight reduction programme if it is accepted by obese children and their parents. Additionally, we suggest, that C-peptide may be useful in monitoring of this therapy. Further studies with longer term intervention are needed to confirm the value of this biochemical marker in the management of prepubertal obese children.
authors.affiliation: Zakład Badań Przesiewowych, Instytut Matki i Dziecka w Warszawie. biochem.imid@imid.med.pl
authors.forename: Joanna
authors.initials: J
authors.lastname: Gajewska
authors.forename: Witold
authors.initials: W
authors.lastname: Klemarczyk
authors.forename: Jadwiga
authors.initials: J
authors.lastname: Ambroszkiewicz
authors.forename: Magdalena
authors.initials: M
authors.lastname: Chełchowska
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Riahi
authors.forename: Anna
authors.initials: A
authors.lastname: Zielińska
authors.forename: Mariusz
authors.initials: M
authors.lastname: Ołtarzewski
authors.forename: Teresa
authors.initials: T
authors.lastname: Laskowska-Klita
mesh_terms: D000886:Anthropometry; D015415:Biomarkers / Q000378:metabolism; D015992:Body Mass Index; D002096:C-Peptide / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D008076:Cholesterol, HDL / Q000378:metabolism; D008078:Cholesterol, LDL / Q000378:metabolism; D015444:Exercise; D005260:Female; D006801:Humans; D050356:Lipid Metabolism; D008297:Male; D009765:Obesity / Q000378:metabolism* / Q000534:rehabilitation*; D040242:Risk Reduction Behavior; D014280:Triglycerides / Q000378:metabolism; D015431:Weight Loss
chemical_list: D015415:Biomarkers; D002096:C-Peptide; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D014280:Triglycerides