title: Histone deacetyltransferase1 expression in mouse oocyte and their in vitro-fertilized embryo: effect of oocyte vitrification.
abstract: This study was conducted to investigate the expression of Histone Deacetyltransferase1 (HDAC1) in mouse embryos derived from the vitrified-warmed oocytes. Firstly, the mouse oocytes at metaphaseII (MII) stage were randomly allocated into three groups: A untreated (control), B exposed to vitriﬁcation solution (VS) without being plunged into liquid nitrogen (toxicity), or C vitriﬁed by open-pulled straw (OPS) method (vitriﬁcation). After warming, they were fertilized in vitro. Fresh oocytes were used as control. Expression of HDAC1 was then examined in MII mouse oocytes and embryos by immunofluorescence with anti-HDAC1 polyclonal antibody and fluorescein isothiocyanate-conjugated goat anti-rabbit IgG. Results showed that after in vitro fertilization (IVF), developmental rates to two-cell embryos (39%), 4-cell embryos (35%), morula (32%) and blastocysts (26%) in cryopreserved oocytes were all significantly lower than those of fresh oocytes (P < 0.01). In addition, HDAC1 expression in the vitrified group was significantly lower (P< 0.05) than that in the control and toxicity groups at all developmental stages except for the blastocyst. Moreover, the vitrified-warmed oocytes showed significantly lower (P < 0.05) HDAC1 expression compared with that of control and toxicity groups. In conclusion, HDAC1 was expressed both in oocytes and in their in vitro-fertilized embryos. This decreased expression of HDAC1 in mouse oocytes and the embryos due to the cryopreservation may have a negative impact on embryo development.
authors.affiliation: College of Animal Science and Technology, Agricultural University of Hebei, Baoding, China.
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Pei
authors.forename: Guang-Bin
authors.initials: GB
authors.lastname: Zhou
authors.forename: Lun
authors.initials: L
authors.lastname: Suo
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Wang
authors.forename: Guo-Quan
authors.initials: GQ
authors.lastname: Wu
authors.forename: Xiang-Wei
authors.initials: XW
authors.lastname: Fu
authors.forename: Yun-Peng
authors.initials: YP
authors.lastname: Hou
authors.forename: Shi-En
authors.initials: SE
authors.lastname: Zhu
mesh_terms: D000818:Animals; D003080:Cold Temperature; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism*; D005260:Female; D005307:Fertilization in Vitro / Q000009:adverse effects; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D051548:Histone Acetyltransferases / Q000235:genetics / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D008677:Metaphase; D051379:Mice; D009028:Morula / Q000166:cytology / Q000378:metabolism*; D054315:Oocyte Retrieval; D009865:Oocytes / Q000166:cytology / Q000378:metabolism*; D013094:Spermatozoa; D058989:Vitrification
chemical_list: D051548:Histone Acetyltransferases