title: Peroxotitanate- and monosodium metal-titanate compounds as inhibitors of bacterial growth.
abstract: Sodium titanates are ion-exchange materials that effectively bind a variety of metal ions over a wide pH range. Sodium titanates alone have no known adverse biological effects but metal-exchanged titanates (or metal titanates) can deliver metal ions to mammalian cells to alter cell processes in vitro. In this work, we test a hypothesis that metal-titanate compounds inhibit bacterial growth; demonstration of this principle is one prerequisite to developing metal-based, titanate-delivered antibacterial agents. Focusing initially on oral diseases, we exposed five species of oral bacteria to titanates for 24 h, with or without loading of Au(III), Pd(II), Pt(II), and Pt(IV), and measuring bacterial growth in planktonic assays through increases in optical density. In each experiment, bacterial growth was compared with control cultures of titanates or bacteria alone. We observed no suppression of bacterial growth by the sodium titanates alone, but significant (p < 0.05, two-sided t-tests) suppression was observed with metal-titanate compounds, particularly Au(III)-titanates, but with other metal titanates as well. Growth inhibition ranged from 15 to 100% depending on the metal ion and bacterial species involved. Furthermore, in specific cases, the titanates inhibited bacterial growth 5- to 375-fold versus metal ions alone, suggesting that titanates enhanced metal-bacteria interactions. This work supports further development of metal titanates as a novel class of antibacterials.
authors.affiliation: Department of Oral Biology, University of Washington, Seattle, Washington 98195, USA.
authors.forename: Whasun O
authors.initials: WO
authors.lastname: Chung
authors.forename: John C
authors.initials: JC
authors.lastname: Wataha
authors.forename: David T
authors.initials: DT
authors.lastname: Hobbs
authors.forename: Jonathan
authors.initials: J
authors.lastname: An
authors.forename: Jacqueline J
authors.initials: JJ
authors.lastname: Wong
authors.forename: Christine H
authors.initials: CH
authors.lastname: Park
authors.forename: Sami
authors.initials: S
authors.lastname: Dogan
authors.forename: Mark C
authors.initials: MC
authors.lastname: Elvington
authors.forename: R Bruce
authors.initials: RB
authors.lastname: Rutherford
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D001424:Bacterial Infections / Q000188:drug therapy; D004337:Drug Carriers / Q000737:chemistry*; D008670:Metals / Q000008:administration & dosage* / Q000494:pharmacology*; D010087:Oxides / Q000737:chemistry*; D014025:Titanium / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D004337:Drug Carriers; D008670:Metals; D010087:Oxides; C525208:peroxo-titanate; C471701:sodium titanate; D014025:Titanium