title: Electron capture, collision-induced, and electron capture-collision induced dissociation in Q-TOF.
abstract: Recently, we demonstrated that a radio-frequency-free electromagnetostatic (rf-free EMS) cell could be retrofitted into a triple quad mass spectrometer to allow electron-capture dissociation (ECD) without the aid of cooling gas or phase-specific electron injection into the cell (Voinov et al., Rapid Commun Mass Spectrom 22, 3087-3088, 2008; Voinov et al., Anal Chem 81, 1238-1243, 2009). Subsequently, we used our rf-free EMS cell in the same instrument platform to demonstrate ECD occurring in the same space and at the same time with collision-induced dissociation (CID) to produce golden pairs and even triplets from peptides (Voinov et al., Rapid Commun Mass Spectrom 23, 3028-3030, 2009). In this report, we demonstrate that ECD and CID product-ion mass spectra can be recorded at high resolution with flexible control of fragmentation processes using a newly designed cell installed in a hybrid Q-TOF tandem mass spectrometer.
authors.affiliation: Department of Chemistry, Oregon State University, Corvallis, OR 97331, USA. valery.voinov@orst.edu
authors.forename: Valery G
authors.initials: VG
authors.lastname: Voinov
authors.forename: Max L
authors.initials: ML
authors.lastname: Deinzer
authors.forename: Joseph S
authors.initials: JS
authors.lastname: Beckman
authors.forename: Douglas F
authors.initials: DF
authors.lastname: Barofsky
mesh_terms: D004583:Electrons; D009842:Oligopeptides / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D009842:Oligopeptides