title: Molecular discrimination of sheep bovine spongiform encephalopathy from scrapie.
abstract: Sheep CH1641-like transmissible spongiform encephalopathy isolates have shown molecular similarities to bovine spongiform encephalopathy (BSE) isolates. We report that the prion protein PrPSc from sheep BSE is extremely resistant to denaturation. This feature, combined with the N-terminal PrPSc cleavage, allowed differentiation of classical scrapie, including CH1641-like, from natural goat BSE and experimental sheep BSE.
authors.affiliation: Istituto Superiore di Sanita, Rome, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Pirisinu
authors.forename: Sergio
authors.initials: S
authors.lastname: Migliore
authors.forename: Michele Angelo
authors.initials: MA
authors.lastname: Di Bari
authors.forename: Elena
authors.initials: E
authors.lastname: Esposito
authors.forename: Thierry
authors.initials: T
authors.lastname: Baron
authors.forename: Claudia
authors.initials: C
authors.lastname: D'Agostino
authors.forename: Luigi
authors.initials: L
authors.lastname: De Grossi
authors.forename: Gabriele
authors.initials: G
authors.lastname: Vaccari
authors.forename: Umberto
authors.initials: U
authors.lastname: Agrimi
authors.forename: Romolo
authors.initials: R
authors.lastname: Nonno
mesh_terms: D000818:Animals; D002417:Cattle; D016643:Encephalopathy, Bovine Spongiform / Q000175:diagnosis* / Q000378:metabolism; D018620:PrPSc Proteins / Q000378:metabolism; D011489:Protein Denaturation; D055550:Protein Stability; D012608:Scrapie / Q000175:diagnosis* / Q000378:metabolism; D012756:Sheep; D012995:Solubility
chemical_list: D018620:PrPSc Proteins