title: Proteomic analysis of pancreatic intraepithelial neoplasia and pancreatic carcinoma in rat models.
abstract: OBJECTIVE
  To detect the proteomic variabilities of pancreatic intraepithelial neoplasia (PanIN) and pancreatic carcinoma (PC) induced by 7,12-dimethylbenzanthracene (DMBA) in rat models and to identify potential biomarkers.
  
  
  METHODS
  Sixty adult male Sprague Dawley rats were randomized into three groups. The rats had DMBA implanted into their pancreas for one (n = 20) or two months (n = 20) or assigned to the normal group (n = 20). The rats were killed after one or two months, and were evaluated histopathologically. Three tissue samples from each group of rats with either normal pancreas, PanIN (PanIN-2) or PC were examined by 2D-DIGE. The different expression spot features were analyzed by matrix-assisted laser desorption/ionization-time of flight/time of flight (MALDI-TOF/TOF) tandem mass spectrometry. The expression of enolase 1, a differentially expressed protein, was identified by immunohistochemistry.
  
  
  RESULTS
  There was significant difference in the proportions of neoplastic changes between the 1- and 2-mogroups (P = 0.0488). There was an increase in the frequency of adenocarcinomas in the 2-mo group compared with the 1-mo group (P = 0.0309). No neoplastic changes were observed in any of the animals in the normal group. Enolase 1, pancreatic ELA3B, necdin, Hbp23, CHD3, hnRNP A2/B1, Rap80, and Gnb2l1 were up-regulated in the PanIN and PC tissues, and CEL, TPT1, NME2, PCK2, an unnamed protein product, and glycine C-acetyltransferase were down-regulated in the PanIN and PC tissues. The immunohistochemical results showed that enolase 1 expression was up-regulated in the pancreatic cancer tissues of rats and humans.
  
  
  CONCLUSIONS
  The pancreatic protein expression changes induced by DMBA suggest potential molecular targets for the early diagnosis and treatment of PC.
authors.affiliation: Department of Gastroenterology, the Ninth People's Hospital Affiliated to the School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Hai-Lin
authors.initials: HL
authors.lastname: Liu
authors.forename: Ya
authors.initials: Y
authors.lastname: Li
authors.forename: Ping
authors.initials: P
authors.lastname: Yuan
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002277:Carcinoma / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D002278:Carcinoma in Situ / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D016009:Chi-Square Distribution; D015180:Electrophoresis, Gel, Two-Dimensional; D007150:Immunohistochemistry; D008297:Male; D009363:Neoplasm Proteins / Q000378:metabolism*; D010190:Pancreatic Neoplasms / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry; D013997:Time Factors; D000090324:Tumor Protein, Translationally-Controlled 1
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; C000715647:TPT1 protein, human; C000715649:Tpt1 protein, rat; D000090324:Tumor Protein, Translationally-Controlled 1; D015127:9,10-Dimethyl-1,2-benzanthracene; D010751:Phosphopyruvate Hydratase