title: Platelet administration via the portal vein promotes liver regeneration in rats after 70% hepatectomy.
abstract: OBJECTIVE
  This study examines the applicability of platelet infusion therapy for liver regeneration in vivo.
  
  
  BACKGROUND
  We recently reported that platelets accumulate in the liver immediately after extended hepatectomy and promote residual liver regeneration. Liver regeneration depends on the number of accumulated platelets in the sinusoids.
  
  
  METHODS
  Male Sprague-Dawley rats underwent 70% hepatectomy and were then assigned to groups that were infused with 1 mL of either platelet-rich plasma (PRP; 1 × 10(9) platelets/mL) in normal saline (NS) or NS via the portal vein. We then analyzed liver regeneration and the signaling pathways that are related to liver regeneration and function. The dynamics of platelets infused via the portal vein were visualized before and after hepatectomy.
  
  
  RESULTS
  The liver/body weight ratio after 70% hepatectomy was significantly higher and the Ki-67 labeling index was higher in the PRP, than in the NS group. The Akt pathway was activated earlier in the PRP, than in the NS group with concurrent ERK1/2 pathway activation, but this was prolonged in the PRP group. Many more platelets infused via the portal vein accumulated in the sinusoid after 70% hepatectomy, and serum liver function tests and histological findings revealed that portal infusion did not cause liver damage.
  
  
  CONCLUSIONS
  Platelets infused via the portal vein promoted liver regeneration after 70% hepatectomy in rats without liver damage. These findings indicate that PRP administration could be a useful part of liver regeneration therapy.
authors.affiliation: Department of Surgery, Advanced Biomedical Applications, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Japan.
authors.forename: Ryota
authors.initials: R
authors.lastname: Matsuo
authors.forename: Yoritaka
authors.initials: Y
authors.lastname: Nakano
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Ohkohchi
mesh_terms: D000818:Animals; D001707:Biopsy, Needle; D015153:Blotting, Western; D004195:Disease Models, Animal; D006498:Hepatectomy / Q000009:adverse effects / Q000379:methods*; D007150:Immunohistochemistry; D007262:Infusions, Intravenous; D008099:Liver / Q000473:pathology*; D008111:Liver Function Tests; D008115:Liver Regeneration / Q000502:physiology*; D008297:Male; D017713:Platelet Transfusion / Q000379:methods*; D011169:Portal Vein; D011182:Postoperative Care / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function; D012016:Reference Values; D016896:Treatment Outcome