title: Association of TRB3 Q84R polymorphism with polycystic ovary syndrome in Chinese women.
abstract: BACKGROUND
  Tribbles 3 (TRB3) affects insulin signalling by inhibiting insulin-stimulated Akt phosphorylation and subsequent activation. A single nucleotide polymorphism located in the second extron of the human TRB3 gene is thought to be associated with insulin resistance. The latter is a core abnormality in PCOS independent of obesity. The present study was designed to clarify the relationships of TRB3 Q84R polymorphism with PCOS in a Chinese women group.
  
  
  METHODS
  A case-control study with two groups: PCOS group (n = 336) and control group of infertility women for tubal and/or male factor (n = 116) was performed. Genotyping of the TRB3 R84 variant was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).
  
  
  RESULTS
  The frequency of genotype QQ in PCOS women was significantly lower, while genotype QR and RR were significantly higher than that in control group (p < 0.05). However, the difference disappeared after adjustment for BMI. At glucose1h, glucose2h and insulin2h point, the difference between QQ individuals and R84 allele carriers in PCOS women reached statistical significance during OGTT (p < 0.05).
  
  
  CONCLUSIONS
  TRB3 Q84R polymorphism is associated with obesity and especially glucose metabolism and not associated with polycystic ovary syndrome because of compositional characteristics of phenotype in Chinese PCOS women.
authors.affiliation: Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, P.R China.
authors.forename: Xue
authors.initials: X
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Fu
authors.forename: Qiufang
authors.initials: Q
authors.lastname: Zhang
authors.forename: Liying
authors.initials: L
authors.lastname: Yan
authors.forename: Yanmin
authors.initials: Y
authors.lastname: Ma
authors.forename: Binbin
authors.initials: B
authors.lastname: Tu
authors.forename: Nana
authors.initials: N
authors.lastname: Liu
authors.forename: Jie
authors.initials: J
authors.lastname: Qiao
mesh_terms: D000293:Adolescent; D000328:Adult; D019943:Amino Acid Substitution / Q000502:physiology; D001120:Arginine / Q000235:genetics; D044466:Asian People / Q000235:genetics*; D001786:Blood Glucose / Q000378:metabolism; D016022:Case-Control Studies; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000502:physiology; D005260:Female; D020022:Genetic Predisposition to Disease; D055106:Genome-Wide Association Study; D005838:Genotype; D018698:Glutamic Acid / Q000235:genetics; D006801:Humans; D009765:Obesity / Q000208:ethnology / Q000235:genetics / Q000378:metabolism; D011085:Polycystic Ovary Syndrome / Q000208:ethnology / Q000235:genetics* / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000502:physiology / Q000037:antagonists & inhibitors; D012097:Repressor Proteins / Q000235:genetics* / Q000502:physiology; D055815:Young Adult
chemical_list: D001786:Blood Glucose; D018797:Cell Cycle Proteins; D012097:Repressor Proteins; C474831:TRIB3 protein, human; D018698:Glutamic Acid; D001120:Arginine; D017346:Protein Serine-Threonine Kinases