title: Molecular and expression characterization of growth hormone/prolactin family genes in the Prenant's schizothoracin.
abstract: The growth hormone/prolactin family genes are major participants in control of several complex physiologic processes, including growth, reproduction, and metabolism. In this study, total RNA was isolated from the pituitary of the Prenant's schizothoracin (Schizothorax prenanti), and SMART cDNA was synthesized from 100 ng total RNA. The full-length cDNAs of three GH/PRL family genes have been cloned and sequenced from the SMART cDNA library. The growth hormone of S. prenanti (SpGH) cDNA contains 1238 base pairs (bp) and encodes 215 amino acid (aa) residues; the prolactin (SpPRL) cDNA contains 1124 bp and encodes 209 aa residues; the cDNA sequence of somatolactin (SpSL) is 1290 bp in length, and encodes for a peptide of 234 aa residues. Although there is only about 22% aa sequence identity between the three deduced proteins, SpGH, SpPRL and SpSL, overall, the C-terminal region shows a higher identity. Multiple aa sequence alignments indicated the deduced SpGH, SpPRL and SpSL show high identities to that of goldfish and zebrafish, respectively. RT-PCR analysis revealed that the three hormones displayed different tissue distribution patterns. SpPRL transcripts were detected only in the pituitary of the Prenant's schizothoracin. SpSL was more widely distributed than SpPRL, high mRNA levels of SpSL were detected in the pituitary and low mRNA levels of SpSL were found in liver, kidney and heart. SpGH was expressed in nearly all tissues detected with the highest expression level in the pituitary.
authors.affiliation: Key Laboratory of Freshwater Biodiversity Conservation and Utilization, Ministry of Agriculture of China, Yangtze River Fisheries Research Institute, Chinese Academy of Fisheries Science, Jingzhou 434000, China.
authors.forename: Chuangju
authors.initials: C
authors.lastname: Li
authors.forename: Xihua
authors.initials: X
authors.lastname: Chen
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Huan
authors.initials: H
authors.lastname: Ye
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003530:Cyprinidae / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics; D013006:Growth Hormone / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D009928:Organ Specificity / Q000235:genetics; D010802:Phylogeny; D010907:Pituitary Hormones / Q000737:chemistry / Q000235:genetics; D011388:Prolactin / Q000737:chemistry / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment
chemical_list: D018076:DNA, Complementary; D029941:Fish Proteins; D006023:Glycoproteins; D010907:Pituitary Hormones; D012333:RNA, Messenger; C064448:somatolactin protein, fish; D011388:Prolactin; D013006:Growth Hormone