title: Changes in proteomics profile during maturation of marrow-derived dendritic cells treated with oxidized low-density lipoprotein.
abstract: Increasing evidence suggests that dendritic cells (DCs) and oxidized low-density lipoprotein (ox-LDL) participate in atherosclerosis. However, few data on the molecular mechanisms of this process are available. To address this question, we used iTRAQ labeling followed by LC-MS/MS analysis to identify many proteins that changed markedly during the maturation of dendritic cells stimulated with ox-LDL. Among a total of 781 identified proteins, 93 were upregulated and 100 were downregulated. The major and significant changes in upregulated proteins were that ox-LDL not only affected the levels of intracellular cathepsins G, Z, D and S, but also significantly enhanced cathepsin S secretion by the treated cells. Our results may provide clues for a more comprehensive understanding the pathogenesis of atherosclerosis.
authors.affiliation: Department of Cardiology, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang Province, P R China.
authors.forename: Junhua
authors.initials: J
authors.lastname: Ge
authors.forename: Hui
authors.initials: H
authors.lastname: Yan
authors.forename: Shan
authors.initials: S
authors.lastname: Li
authors.forename: Wencheng
authors.initials: W
authors.lastname: Nie
authors.forename: Kan
authors.initials: K
authors.lastname: Dong
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Weiguo
authors.initials: W
authors.lastname: Zhu
authors.forename: Fangyan
authors.initials: F
authors.lastname: Fan
authors.forename: Jianhua
authors.initials: J
authors.lastname: Zhu
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D050197:Atherosclerosis; D015153:Blotting, Western; D001854:Bone Marrow Cells; D002403:Cathepsins / Q000378:metabolism; D002853:Chromatography, Liquid; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D006801:Humans; D007553:Isotope Labeling; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D020543:Proteome / Q000737:chemistry / Q000187:drug effects*; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry
chemical_list: D016207:Cytokines; D008077:Lipoproteins, LDL; D020543:Proteome; C099252:oxidized low density lipoprotein; D002403:Cathepsins; C028217:cathepsin S