title: Membrane-SPINE: an improved method to identify protein-protein interaction partners of membrane proteins in vivo.
abstract: Membrane proteins are crucial for many essential cellular processes. As membrane proteins function in complexes, methods to detect and to characterize membrane protein-protein interactions are undoubtedly required. Therefore, we developed the "Membrane-Strep-tagged protein interaction experiment" (Membrane-SPINE) that combines the specific purification of a Strep-tagged membrane protein with the reversible fixation of protein complexes by formaldehyde cross-linking. In combination with MS analysis, we suggest Membrane-SPINE as a powerful tool to identify unknown interaction partners of membrane proteins in vivo.
authors.affiliation: Department of Biology, Humboldt University Berlin, Berlin, Germany.
authors.forename: Volker S
authors.initials: VS
authors.lastname: Müller
authors.forename: Peter R
authors.initials: PR
authors.lastname: Jungblut
authors.forename: Thomas F
authors.initials: TF
authors.lastname: Meyer
authors.forename: Sabine
authors.initials: S
authors.lastname: Hunke
mesh_terms: D000345:Affinity Labels; D001426:Bacterial Proteins / Q000378:metabolism; D003001:Cloning, Molecular; D005557:Formaldehyde; D015151:Immunoblotting; D013058:Mass Spectrometry; D008565:Membrane Proteins / Q000378:metabolism*; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods*; D011506:Proteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D012486:Salmonella typhimurium; D019809:Streptavidin / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000345:Affinity Labels; D001426:Bacterial Proteins; D008565:Membrane Proteins; D011506:Proteins; D005557:Formaldehyde; D019809:Streptavidin