title: Controlling and switching the morphology of micellar nanoparticles with enzymes.
abstract: Micelles were prepared from polymer-peptide block copolymer amphiphiles containing substrates for protein kinase A, protein phosphatase-1, and matrix metalloproteinases 2 and 9. We examine reversible switching of the morphology of these micelles through a phosphorylation-dephosphorylation cycle and study peptide-sequence directed changes in morphology in response to proteolysis. Furthermore, the exceptional uniformity of these polymer-peptide particles makes them amenable to cryo-TEM reconstruction techniques lending insight into their internal structure.
authors.affiliation: Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093, USA.
authors.forename: Ti-Hsuan
authors.initials: TH
authors.lastname: Ku
authors.forename: Miao-Ping
authors.initials: MP
authors.lastname: Chien
authors.forename: Matthew P
authors.initials: MP
authors.lastname: Thompson
authors.forename: Robert S
authors.initials: RS
authors.lastname: Sinkovits
authors.forename: Norman H
authors.initials: NH
authors.lastname: Olson
authors.forename: Timothy S
authors.initials: TS
authors.lastname: Baker
authors.forename: Nathan C
authors.initials: NC
authors.lastname: Gianneschi
mesh_terms: D017868:Cyclic AMP-Dependent Protein Kinases / Q000737:chemistry / Q000494:pharmacology*; D020778:Matrix Metalloproteinase 2 / Q000737:chemistry / Q000494:pharmacology*; D020780:Matrix Metalloproteinase 9 / Q000737:chemistry / Q000494:pharmacology*; D008823:Micelles; D046529:Microscopy, Electron, Transmission; D008958:Models, Molecular; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D054645:Protein Phosphatase 1 / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D008823:Micelles; D017868:Cyclic AMP-Dependent Protein Kinases; D054645:Protein Phosphatase 1; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9