title: Abscisic acid effects on activity and expression of barley (Hordeum vulgare) plastidial glucose-6-phosphate dehydrogenase.
abstract: Total glucose-6-phosphate dehydrogenase (G6PDH) activity, protein abundance, and transcript levels of G6PDH isoforms were measured in response to exogenous abscisic acid (ABA) supply to barley (Hordeum vulgare cv Nure) hydroponic culture. Total G6PDH activity increased by 50% in roots treated for 12 h with exogenous 0.1 mM ABA. In roots, a considerable increase (35%) in plastidial P2-G6PDH transcript levels was observed during the first 3 h of ABA treatment. Similar protein variations were observed in immunoblotting analyses. In leaves, a 2-fold increase in total G6PDH activity was observed after ABA treatment, probably related to an increase in the mRNA level (increased by 50%) and amount of protein (increased by 85%) of P2-G6PDH. Together these results suggest that the plastidial P2-isoform plays an important role in ABA-treated barley plants.
authors.affiliation: Dipartimento di Biologia Strutturale e Funzionale, Università di Napoli Federico II, Via Cinthia, 80126 Naples, Italy.
authors.forename: Manuela
authors.initials: M
authors.lastname: Cardi
authors.forename: Kamel
authors.initials: K
authors.lastname: Chibani
authors.forename: Donata
authors.initials: D
authors.lastname: Cafasso
authors.forename: Nicolas
authors.initials: N
authors.lastname: Rouhier
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Jacquot
authors.forename: Sergio
authors.initials: S
authors.lastname: Esposito
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology*; D018506:Gene Expression Regulation, Plant; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics* / Q000378:metabolism*; D001467:Hordeum / Q000235:genetics* / Q000378:metabolism*; D018527:Hydroponics; D010802:Phylogeny; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism; D018087:Plastids / Q000201:enzymology; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D020539:Sequence Analysis, Protein
chemical_list: D020033:Protein Isoforms; D000040:Abscisic Acid; D005954:Glucosephosphate Dehydrogenase