title: PDGFRalpha-positive cells in bone marrow are mobilized by high mobility group box 1 (HMGB1) to regenerate injured epithelia.
abstract: The role of bone marrow cells in repairing ectodermal tissue, such as skin epidermis, is not clear. To explore this process further, this study examined a particular form of cutaneous repair, skin grafting. Grafting of full thickness wild-type mouse skin onto mice that had received a green fluorescent protein-bone marrow transplant after whole body irradiation led to an abundance of bone marrow-derived epithelial cells in follicular and interfollicular epidermis that persisted for at least 5 mo. The source of the epithelial progenitors was the nonhematopoietic, platelet-derived growth factor receptor α-positive (Lin(-)/PDGFRα(+)) bone marrow cell population. Skin grafts release high mobility group box 1 (HMGB1) in vitro and in vivo, which can mobilize the Lin(-)/PDGFRα(+) cells from bone marrow to target the engrafted skin. These data provide unique insight into how skin grafts facilitate tissue repair and identify strategies germane to regenerative medicine for skin and, perhaps, other ectodermal defects or diseases.
authors.affiliation: Division of Gene Therapy Science, Osaka University Graduate School of Medicine, Osaka 565-0871, Japan. tamai@gts.med.osaka-u.ac.jp
authors.forename: Katsuto
authors.initials: K
authors.lastname: Tamai
authors.forename: Takehiko
authors.initials: T
authors.lastname: Yamazaki
authors.forename: Takenao
authors.initials: T
authors.lastname: Chino
authors.forename: Masaru
authors.initials: M
authors.lastname: Ishii
authors.forename: Satoru
authors.initials: S
authors.lastname: Otsuru
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Kikuchi
authors.forename: Shin
authors.initials: S
authors.lastname: Iinuma
authors.forename: Kotaro
authors.initials: K
authors.lastname: Saga
authors.forename: Keisuke
authors.initials: K
authors.lastname: Nimura
authors.forename: Takashi
authors.initials: T
authors.lastname: Shimbo
authors.forename: Noriko
authors.initials: N
authors.lastname: Umegaki
authors.forename: Ichiro
authors.initials: I
authors.lastname: Katayama
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Miyazaki
authors.forename: Junji
authors.initials: J
authors.lastname: Takeda
authors.forename: John A
authors.initials: JA
authors.lastname: McGrath
authors.forename: Jouni
authors.initials: J
authors.lastname: Uitto
authors.forename: Yasufumi
authors.initials: Y
authors.lastname: Kaneda
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism*; D016026:Bone Marrow Transplantation; D004817:Epidermis / Q000293:injuries* / Q000378:metabolism*; D006085:Graft Survival / Q000235:genetics; D024243:HMGB1 Protein / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D020796:Receptor, Platelet-Derived Growth Factor alpha / Q000235:genetics / Q000378:metabolism*; D012038:Regeneration; D016038:Skin Transplantation; D014184:Transplantation, Homologous
chemical_list: D024243:HMGB1 Protein; D020796:Receptor, Platelet-Derived Growth Factor alpha