title: Effect of in vivo L-acetylcarnitine administration on ATP-ases enzyme systems of synaptic plasma membranes from rat cerebral cortex.
abstract: The maximum rates (V (max)) of some enzymatic activities related to energy consumption (ATP-ases) were evaluated in two types of synaptic plasma membranes (SPM) isolated from cerebral cortex of rats subjected to in vivo treatment with L: -acetylcarnitine at two different doses (30 and 60 mg kg(-1) i.p., 28 days, 5 days/week). The following enzyme activities were evaluated: acetylcholinesterase (AChE); Na(+), K(+), Mg(2+)-ATP-ase; ouabain insensitive Mg(2+)-ATP-ase; Na(+), K(+)-ATP-ase; direct Mg(2+)-ATP-ase; Ca(2+), Mg(2+)-ATP-ase; Low- and High-affinity Ca(2+)-ATP-ase. Sub-chronic treatment with L: -acetylcarnitine increased Na(+), K(+)-ATP-ase activity on SPM 2 and Ca(2+), Mg(2+)-ATP-ase activity on both SPM fractions. These results suggest (1) that the sensitivity to drug treatment is different between the two populations of SPM, confirming the micro-heterogeneity of these sub-fractions, probably originating from different types of synapses, (2) the specificity of the molecular site of action of the drug on SPM and (3) its interference on ion homeostasis at synaptic level.
authors.affiliation: Department of Forensic Medicine and Pharmacological-Toxicological Sciences, Division of Pharmacological and Toxicological Sciences, Laboratory of Pharmacology and Molecular Medicine of Central Nervous System, University of Pavia, Via Ferrata, 9, 27100, Pavia, Italy. robertofederico.villa@unipv.it
authors.forename: Roberto Federico
authors.initials: RF
authors.lastname: Villa
authors.forename: Federica
authors.initials: F
authors.lastname: Ferrari
authors.forename: Antonella
authors.initials: A
authors.lastname: Gorini
mesh_terms: D000108:Acetylcarnitine / Q000494:pharmacology*; D000110:Acetylcholinesterase / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D002540:Cerebral Cortex / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D004734:Energy Metabolism / Q000187:drug effects; D005260:Female; D018697:Nootropic Agents / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013570:Synaptic Membranes / Q000187:drug effects* / Q000201:enzymology*
chemical_list: D018697:Nootropic Agents; D000108:Acetylcarnitine; D000110:Acetylcholinesterase; D000251:Adenosine Triphosphatases