title: Comparison of contents of five ephedrine alkaloids in three official origins of Ephedra Herb in China by high-performance liquid chromatography.
abstract: In the present paper, a fast and economical HPLC method [on a Phenomenex Polar-RP column with a solution of (phosphoric acid:triethylamine:dibutylamine:water = 0.40:0.1:0.2:499.3) and methanol] is developed, and applied for the determination of norephedrine, norpseudoephedrine, ephedrine (E), pseudoephedrine (PE) and methylephedrine (ME) in 64 samples of three species from main habitats in China. Quantitation data showed that total alkaloid content in Ephedra equisetina Bge. (2.708 ± 0.642%) is higher than that in E. sinica Stapf. (1.365 ± 0.624%) and E. intermedia Schrenk et C. A. Mey. (1.537 ± 0.746%), but the range of total alkaloid content in each species is so wide that the ranges of the three species greatly overlap. The contents of E, PE and ME are different among the three species. The ratio E/total alkaloid content and ratio E/PE as well as E and ME contents can be used for identification of E. sinica, E. intermedia and E. equisetina from one another.
authors.affiliation: School of Pharmaceutical Sciences, Peking University, Beijing, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Hong
authors.forename: Hu-Biao
authors.initials: HB
authors.lastname: Chen
authors.forename: Dong-Hui
authors.initials: DH
authors.lastname: Yang
authors.forename: Ming-Ying
authors.initials: MY
authors.lastname: Shang
authors.forename: Xuan
authors.initials: X
authors.lastname: Wang
authors.forename: Shao-Qing
authors.initials: SQ
authors.lastname: Cai
authors.forename: Masayuki
authors.initials: M
authors.lastname: Mikage
mesh_terms: D000470:Alkaloids / Q000737:chemistry*; D002681:China; D002851:Chromatography, High Pressure Liquid; D029789:Ephedra / Q000737:chemistry*; D004809:Ephedrine / Q000031:analogs & derivatives / Q000737:chemistry*; D010665:Phenylpropanolamine / Q000737:chemistry
chemical_list: D000470:Alkaloids; C005331:norpseudoephedrine; D010665:Phenylpropanolamine; C041707:N-methylephedrine; D004809:Ephedrine