title: An automated colorimetric method for the estimation of lactate dehydrogenase activity in serum.
abstract: An automated method for estimating lactate dehydrogenase (LDH) in serum is presented. Fe3+ is reduced to Fez+ by NADH formed when lactate is oxidized to pyruvate. Fe2+ complexed with 2,2-bipyridyl is measured colorimetrically. Solutions of Fe2+ salt are used as standards. Nicotinamide is used to stabilize NAD+ in solution and is shown to enhance enzyme activity. The method is less expensive than most automated methods published and reagents are stable for at least 4 weeks. Results correlate well with a kinetic spectrophotometric method.
authors.affiliation: Landspitalinn, Dept. of Clinical Biochemistry, Reykjavik, Iceland.
authors.forename: M
authors.initials: M
authors.lastname: Kjeld
mesh_terms: D000328:Adult; D000368:Aged; D003124:Colorimetry / Q000379:methods*; D005260:Female; D006801:Humans; D007501:Iron / Q000737:chemistry; D007770:L-Lactate Dehydrogenase / Q000097:blood*; D008297:Male; D008875:Middle Aged; D009536:Niacinamide / Q000737:chemistry
chemical_list: D009536:Niacinamide; D007501:Iron; D007770:L-Lactate Dehydrogenase