title: Preparation and characterization of thermosensitive pluronic F127-b-poly(ɛ-caprolactone) mixed micelles.
abstract: The mixed micelles composed of pluronic F127-b-poly(ɛ-caprolactone) (F127-CL) and bovine serum albumin (BSA) or polylactic acid (PLA) were fabricated for application as promising drug carriers. F127-CL copolymers were characterized by (1)H NMR, FT-IR, GPC, DSC, XRD and POM. They can self-assemble into micelles in water by solvent evaporation method. The thermo-responsivities of the pure and mixed micelles were investigated. The drug release behaviors were investigated in phosphate-buffered solution (PBS) and acetate buffer solution (ABS), respectively, at 37°C. The hemolysis and coagulation assay and the tumor cell growth inhibition assays were further evaluated. The morphologies of pure micelles underwent from the coexistence of the rods and spheres to the spheres with increasing the lengths of CL. The micelle behaviors were influenced with the addition of BSA and PLA. Both pure and mixed micelles of F127-CL with CL length of 200 show thermo-responsivities from 25 to 45°C, while form larger aggregations at high temperature. The hemolysis and coagulation assays showed that the micelles possess good blood compatibility. The cytotoxicity results showed that the copolymer was a safe carrier and the encapsulated doxorubicind.HCl remained its potent anti-tumor effect. The in vitro release profiles displayed a sustained release of DOX.HCl from the micelles. The block copolymers can be great potential as a nanocontainer in drug delivery systems.
authors.affiliation: Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, PR China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhou
authors.forename: Zhao
authors.initials: Z
authors.lastname: Zhang
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
authors.forename: Xing
authors.initials: X
authors.lastname: Guo
authors.forename: Shaobing
authors.initials: S
authors.lastname: Zhou
mesh_terms: D000818:Animals; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004317:Doxorubicin / Q000737:chemistry / Q000493:pharmacokinetics; D004337:Drug Carriers / Q000009:adverse effects / Q000737:chemistry*; D006461:Hemolysis / Q000187:drug effects; D056945:Hep G2 Cells; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D008823:Micelles; D020442:Poloxamer / Q000737:chemistry*; D011091:Polyesters; D011108:Polymers / Q000009:adverse effects / Q000737:chemistry*; D051381:Rats
chemical_list: D004337:Drug Carriers; D008823:Micelles; D011091:Polyesters; D011108:Polymers; D020442:Poloxamer; D019344:Lactic Acid; C033616:poly(lactide); D004317:Doxorubicin