title: Direct chemiluminescence determination of penicillin G potassium and a chemometrical optimization approach.
abstract: A highly selective and simple chemiluminescence (CL) method for determination of penicillin G potassium (PGK) was developed. In the proposed method, CL was elicited from PGK upon its oxidation with H(2)O(2). The light emission was enhanced in the presence of N-cetyl-N,N,N-trimethylammonium bromide (CTMAB). An experimental design, central composite design (CCD), was used to realize the optimized variables, including pH, surfactant (CTMAB) and H(2)O(2) concentrations. Under optimum condition, the calibration graph was linear in the range 3.3 × 10(-3) -3.3 × 10(-1) mmol/L, with a detection limit of 8.8 × 10(-4) mmol/L for PGK. The precision was calculated by analysing samples containing 1.6 × 10(-1) mmol/L PGK (n = 5) and the relative standard deviation (RSD) was 1.40%. The utility of this method was demonstrated by determining PGK in pharmaceutical formulations for injection. The proposed method was validated by a reference method.
authors.affiliation: Analytical Chemistry Department, Faculty of Chemistry, University of Tabriz, Iran. soruraddin@tabrizu.ac.ir
authors.forename: Mohammad H
authors.initials: MH
authors.lastname: Sorouraddin
authors.forename: Mortaza
authors.initials: M
authors.lastname: Iranifam
authors.forename: Abdolhossein
authors.initials: A
authors.lastname: Naseri
authors.forename: Mohammad
authors.initials: M
authors.lastname: Fadakar-Sardroud
authors.forename: Hossein
authors.initials: H
authors.lastname: Gharari-Alibabalou
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D049449:Luminescence; D010400:Penicillin G / Q000032:analysis*; D015203:Reproducibility of Results
chemical_list: D000900:Anti-Bacterial Agents; D010400:Penicillin G