title: Toxicity of graphene in normal human lung cells (BEAS-2B).
abstract: Graphite nanomaterials such as thermally exfoliated graphite oxide (GO) are versatile in many applications. However, little is known about its effects on biological systems. In this study we characrerized the GO using dynamic light scattering (DLS) along with the toxicological aspects related to cytotoxicity and apoptosis in normal human lung cells (BEAS-2B). A significant concentration and time dependent decrease in cell viability was observed at different concentrations (10-100 microg/ml) by the MTT assay after 24 and 48 h of exposure and significant increase of early and late apoptotic cells was observed as compared to control cells. Our study demonstrates that GO induces cytotoxicity and apoptosis in human lung cells.
authors.affiliation: Nanomaterial Toxicology Group, Indian Institute of Toxicology Research (CSIR), P.O. Box 80, M. G. Marg, Lucknow 226001, India.
authors.forename: N V Srikanth
authors.initials: NV
authors.lastname: Vallabani
authors.forename: Sandeep
authors.initials: S
authors.lastname: Mittal
authors.forename: Ritesh K
authors.initials: RK
authors.lastname: Shukla
authors.forename: Alok K
authors.initials: AK
authors.lastname: Pandey
authors.forename: Sanjay R
authors.initials: SR
authors.lastname: Dhakate
authors.forename: Renu
authors.initials: R
authors.lastname: Pasricha
authors.forename: Alok
authors.initials: A
authors.lastname: Dhawan
mesh_terms: D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006108:Graphite / Q000633:toxicity*; D006801:Humans; D008168:Lung / Q000187:drug effects* / Q000502:physiology*; D008422:Materials Testing; D053758:Nanoparticles / Q000633:toxicity*; D010316:Particle Size
chemical_list: D006108:Graphite