title: Immobilisation of linear and cyclic RGD-peptides on titanium surfaces and their impact on endothelial cell adhesion and proliferation.
abstract: Functional coatings on titanium vascular stents and endosseous dental implants could probably enhance endothelial cell (EC) adhesion and activity with a shortening of the wound healing time and an increase of peri-implant angiogenesis during early bone formation. Therefore, the role of the structure of linear and cyclic cell adhesive peptides Arg-Gly-Asp (l-RGD and c-RGD) on differently pre-treated titanium (Ti) surfaces (untreated, silanised vs. functionalised with l- and c-RGD peptides) on EC cell coverage and proliferation was evaluated. After 24 h and after 3 d, surface coverage of adherent cells was quantified and an alamarBlue® proliferation assay was conducted. After 24 h, l-RGD modified surfaces showed a significantly better coverage of adhered cells than untreated titanium (p=0.01). Differences between l-RGD surfaces and silanised Ti (p=0.066) as well as between l-RGD and c-RGD surfaces (p=0.191) were not significant. After 3 d, c-RGD surfaces showed a significantly higher cell coverage than untreated Ti, silanised and l-RGD titanium surfaces (all p<0.0001). After 24 h, c-RGD modified surfaces showed significant higher cell proliferation compared to untreated Ti (p=0.003). However, there were no differences in proliferation between c-RGD and l-RGD (p=0.126) or c-RGD and silanised titanium (p=0.196). After 3 d, proliferation on c-RGD surfaces outranged significantly untreated titanium (p=0.004), silanised (p=0.001) and l-RGD surfaces (p=0.023), whereas no significant difference could be found between untreated Ti and l-RGD surfaces (p=0.54). According to these results, the biomimetic coating of c-RGD peptides on conventional titanium surfaces showed a positive effect on EC cell coverage and proliferation. We were able to show that modifications of titanium surfaces with c-RGD are a promising approach in promoting endothelial cell growth.
authors.affiliation: Department of Oral and Maxillofacial Surgery, University Medical Centre of the Johannes Gutenberg-University Mainz, Mainz, Germany. peer.kaemmerer@unimedizin-mainz.de
authors.forename: P W
authors.initials: PW
authors.lastname: Kämmerer
authors.forename: M
authors.initials: M
authors.lastname: Heller
authors.forename: J
authors.initials: J
authors.lastname: Brieger
authors.forename: M O
authors.initials: MO
authors.lastname: Klein
authors.forename: B
authors.initials: B
authors.lastname: Al-Nawas
authors.forename: M
authors.initials: M
authors.lastname: Gabriel
mesh_terms: D000328:Adult; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology; D042783:Endothelial Cells / Q000187:drug effects*; D005260:Female; D006801:Humans; D008297:Male; D008855:Microscopy, Electron, Scanning; D009842:Oligopeptides / Q000737:chemistry / Q000494:pharmacology*; D013499:Surface Properties; D013997:Time Factors; D014025:Titanium / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D020099:Coated Materials, Biocompatible; D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid; D014025:Titanium