title: High copper levels promotes broiler hepatocyte mitochondrial permeability transition in vivo and in vitro.
abstract: This study was to examine the effects of copper on the mitochondrial non-specific pore. Three hundred sixty, one-day-old, healthy Arbor Acres (AA) broilers were fed with different concentrations (11, 110, 220, and 330 mg/kg) of copper originated from copper sulfate, tribasic copper chloride (TBCC), or copper methionine. At the indicated time point, the mitochondrial permeability transition (MPT) and copper concentration were analyzed. Results showed that under the same copper concentration, the MPT of broilers fed copper methionine was the greatest, followed by TBCC, then copper sulfate. The effects of copper on MPT were time- and dose-dependent. Furthermore, in vitro in the presence of K(+), 5 μM Cu(2+) could cause permeability transition as compared to 10 μM Cu(2+) in buffer without K(+). Taking these results together, we have shown that hepatocellular MPT may be influenced not only by source and concentration of copper or the raising period of broilers, but also by the existence of K(+).
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Rongsheng
authors.initials: R
authors.lastname: Su
authors.forename: Rongmei
authors.initials: R
authors.lastname: Wang
authors.forename: Huabin
authors.initials: H
authors.lastname: Cao
authors.forename: Jiaqiang
authors.initials: J
authors.lastname: Pan
authors.forename: Lijun
authors.initials: L
authors.lastname: Chen
authors.forename: Chengmei
authors.initials: C
authors.lastname: Li
authors.forename: Dayou
authors.initials: D
authors.lastname: Shi
authors.forename: Zhaoxin
authors.initials: Z
authors.lastname: Tang
mesh_terms: D000818:Animals; D002570:Ceruloplasmin / Q000187:drug effects / Q000378:metabolism; D002645:Chickens; D003300:Copper / Q000008:administration & dosage / Q000378:metabolism / Q000493:pharmacokinetics / Q000494:pharmacology*; D019327:Copper Sulfate / Q000493:pharmacokinetics / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D006861:Hydrogen Peroxide / Q000378:metabolism; D007202:Indicators and Reagents; D008715:Methionine / Q000378:metabolism; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D015233:Models, Statistical; D009942:Organometallic Compounds / Q000378:metabolism; D010539:Permeability / Q000187:drug effects
chemical_list: D007202:Indicators and Reagents; D009942:Organometallic Compounds; C042079:copper methionate; D003300:Copper; D008715:Methionine; D006861:Hydrogen Peroxide; D002570:Ceruloplasmin; D019327:Copper Sulfate; C029892:cupric chloride