title: Imaging surface plasmon resonance for multiplex microassay sensing of mycotoxins.
abstract: A prototype imaging surface plasmon resonance-based multiplex microimmunoassay for mycotoxins is described. A microarray of mycotoxin-protein conjugates was fabricated using a continuous flow microspotter device. A competitive inhibition immunoassay format was developed for the simultaneous detection of deoxynivalenol (DON) and zearalenone (ZEN), using a single sensor chip. Initial in-house validation showed limits of detection of 21 and 17 ng/mL for DON and 16 and 10 ng/mL for ZEN in extracts, which corresponds to 84 and 68 μg/kg for DON and 64 and 40 μg/kg for ZEN in maize and wheat samples, respectively. Finally, the results were critically compared with data obtained from liquid chromatography-mass spectrometry confirmatory analysis method and found to be in good agreement. The described multiplex immunoassay for the rapid screening of several mycotoxins meets European Union regulatory limits and represents a robust platform for mycotoxin analysis in food and feed samples.
authors.affiliation: Laboratory of Organic Chemistry, Wageningen University, The Netherlands.
authors.forename: Denis
authors.initials: D
authors.lastname: Dorokhin
authors.forename: Willem
authors.initials: W
authors.lastname: Haasnoot
authors.forename: Maurice C R
authors.initials: MC
authors.lastname: Franssen
authors.forename: Han
authors.initials: H
authors.lastname: Zuilhof
authors.forename: Michel W F
authors.initials: MW
authors.lastname: Nielen
mesh_terms: D007118:Immunoassay / Q000379:methods; D057230:Limit of Detection; D009183:Mycotoxins / Q000032:analysis*; D020349:Surface Plasmon Resonance / Q000379:methods*; D014255:Trichothecenes / Q000032:analysis*; D014908:Triticum / Q000382:microbiology; D003313:Zea mays / Q000382:microbiology; D015025:Zearalenone / Q000032:analysis*
chemical_list: D009183:Mycotoxins; D014255:Trichothecenes; D015025:Zearalenone; C007262:deoxynivalenol