title: Site-specific induction of intestinal hypoxia-inducible factor-1α after hemorrhagic shock.
abstract: The intestine is a major target organ in hemorrhagic shock (HS)-induced tissue injury. Hypoxia-inducible factor (HIF)-1α is the primary transcription factor responsible for regulating cellular response to changes in oxygen tension. Since HS is an acute hypoxic insult, the present study examined changes in the gene expression of HIF-1α in various regions of the intestine, as well as the distribution of HIF-1α protein in the intestinal cells of a rat model of HS. Levels of HIF-1α mRNA were marginally detectable in the intestine of sham-operated control animals, but obviously induced following HS. Duodenal, jejunal and colonic levels of HIF-1α mRNA robustly increased and reached a maximum during the ischemic phase of HS, followed by a rapid decrease almost to control levels during the early phase of resuscitation. The induction of HIF-1α mRNA was maximal in the duodenum. In contrast to the duodenum, jejunum and colon, in the ileum the HIF-1α mRNA level did not increase after HS. Consistent with enhanced HIF-1α gene expression, HIF-1α protein was expressed in the mucosal cells of the duodenum, jejunum and colon, but not in the ileum following HS. These findings indicate that intestinal HIF-1α expression was up-regulated at both the transcriptional and protein level in a site-specific manner in this rat model of HS. Differential regulation of HIF-1α expression along the longitudinal axes of the intestine might be a determinant of the adaptive response to HS-induced intestinal damage.
authors.affiliation: Department of Anesthesiology and Resuscitology, Okayama University Medical School, Okayama 700-8558, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nishie
authors.forename: Toru
authors.initials: T
authors.lastname: Takahashi
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Inoue
authors.forename: Hiroko
authors.initials: H
authors.lastname: Shimizu
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Morimatsu
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Toda
authors.forename: Emiko
authors.initials: E
authors.lastname: Omori
authors.forename: Reiko
authors.initials: R
authors.lastname: Akagi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Katayama
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Morita