title: Matrix pre-coated MALDI MS targets for small molecule imaging in tissues.
abstract: A new sample preparation method for MALDI tissue imaging has been developed for the analysis of low molecular weight compounds that employs matrix pre-coated MALDI targets. Tissue sections need only to be transferred onto the pre-coated target before analysis for fast and easy sample preparation. Pre-coated targets have a homogenous matrix coating with uniform crystals of approximately 1-2 μm and do not require solvents that may lead to analyte delocalization within a tissue section. We report here the use of matrix pre-coated targets for imaging of lipids, peptides, and pharmaceuticals in tissues.
authors.affiliation: Departments of Chemistry, Biochemistry, and the Mass Spectrometry Research Center, Vanderbilt University, MRB3, 465 21st Ave S, Nashville, TN 37235, USA.
authors.forename: Kerri J
authors.initials: KJ
authors.lastname: Grove
authors.forename: Sara L
authors.initials: SL
authors.lastname: Frappier
authors.forename: Richard M
authors.initials: RM
authors.lastname: Caprioli
mesh_terms: D000818:Animals; D005260:Female; D005841:Gentisates; D006651:Histocytochemistry / Q000379:methods*; D008055:Lipids / Q000032:analysis; D051379:Mice; D008810:Mice, Inbred C57BL; D008855:Microscopy, Electron, Scanning; D057054:Molecular Imaging / Q000379:methods*; D010455:Peptides / Q000032:analysis; D004364:Pharmaceutical Preparations / Q000032:analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D005841:Gentisates; D008055:Lipids; D010455:Peptides; D004364:Pharmaceutical Preparations; C010925:2,5-dihydroxybenzoic acid