title: Prognostic significance of matrix metalloproteinase-20 overexpression in laryngeal squamous cell carcinoma.
abstract: CONCLUSIONS
  Matrix metalloproteinase (MMP)-20 is overexpressed in laryngeal squamous cell carcinoma (LSCC) compared with the adjacent normal laryngeal epithelium and MMP-20 plays a role in lymph node metastasis. Overexpression of MMP-20 may be used as a significant prognostic factor for lymph node metastasis. All the findings indicate that MMP-20 may play a role in the initiation and progression of LSCC.
  
  
  OBJECTIVE
  The MMPs are a gene family of zinc-dependent endopeptidases that have been implicated in tumor invasion and metastasis, and MMP-20 is a new member of the MMP family. The purpose of this study was to investigate whether MMP-20 is overexpressed in human LSCC and, if so, the significance of its overexpression in relation to clinical parameters.
  
  
  METHODS
  We analyzed 33 cases of LSCC with RT-PCR and 73 cases of LSCC with immunohistochemistry compared with normal laryngeal epithelium.
  
  
  RESULTS
  We found that MMP-20 is overexpresssed in LSCC compared with the adjacent normal laryngeal epithelium.
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, First Affiliated Hospital of Anhui Medical University, Hefei, Anhui Province, China.
authors.forename: Yehai
authors.initials: Y
authors.lastname: Liu
authors.forename: Yifan
authors.initials: Y
authors.lastname: Li
authors.forename: Zhongmin
authors.initials: Z
authors.lastname: Liu
authors.forename: Liyong
authors.initials: L
authors.lastname: Zhang
authors.forename: Matti
authors.initials: M
authors.lastname: Anniko
authors.forename: Maoli
authors.initials: M
authors.lastname: Duan
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000096:biosynthesis / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000201:enzymology / Q000235:genetics* / Q000556:secondary; D018450:Disease Progression; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D007822:Laryngeal Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D008207:Lymphatic Metastasis / Q000235:genetics; D008297:Male; D053519:Matrix Metalloproteinase 20 / Q000096:biosynthesis / Q000235:genetics*; D008875:Middle Aged; D011379:Prognosis; D012334:RNA, Neoplasm / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; D012334:RNA, Neoplasm; D053519:Matrix Metalloproteinase 20