title: T cell deficiency does not reduce lesions in mice produced by intracerebral injection of NMO-IgG and complement.
abstract: We reported recently that intracerebral administration of NMO-IgG with human complement produces neuromyelitis optica (NMO) lesions in mice. We examined the role of T cells in the formation of NMO lesions by comparing brain histopathology in wildtype and nude mice. Brains were co-injected with IgG from NMO patients and human complement. At 24h and 5days, wildtype vs. nude mouse brains had comparable inflammation (CD45 immunoreactivity), loss of myelin (Luxol Fast Blue staining) and loss of AQP4 immunoreactivity. We conclude that T cells are not required for the formation of NMO lesions in this mouse model.
authors.affiliation: Academic Neurosurgery Unit, St. George's, University of London, London SW170RE, UK.
authors.forename: Samira
authors.initials: S
authors.lastname: Saadoun
authors.forename: Patrick
authors.initials: P
authors.lastname: Waters
authors.forename: Claire
authors.initials: C
authors.lastname: Macdonald
authors.forename: Leslie R
authors.initials: LR
authors.lastname: Bridges
authors.forename: B Anthony
authors.initials: BA
authors.lastname: Bell
authors.forename: Angela
authors.initials: A
authors.lastname: Vincent
authors.forename: A S
authors.initials: AS
authors.lastname: Verkman
authors.forename: Marios C
authors.initials: MC
authors.lastname: Papadopoulos
mesh_terms: D000818:Animals; D001921:Brain / Q000276:immunology / Q000473:pathology; D003165:Complement System Proteins / Q000008:administration & dosage*; D006801:Humans; D007074:Immunoglobulin G / Q000008:administration & dosage*; D007153:Immunologic Deficiency Syndromes / Q000276:immunology* / Q000473:pathology; D007276:Injections, Intraventricular; D051379:Mice; D018345:Mice, Knockout; D008819:Mice, Nude; D009471:Neuromyelitis Optica / Q000276:immunology* / Q000473:pathology; D013601:T-Lymphocytes / Q000276:immunology / Q000473:pathology*
chemical_list: D007074:Immunoglobulin G; D003165:Complement System Proteins