title: [The effect of Aldosterone on the proliferation and collagen production of cardiac fibroblasts].
abstract: OBJECTIVE
  To investigate the effect of aldosterone (ALD) on the proliferation of fetal cardiac fibroblasts (FCFS) and the production of collagen I and collagen III in FCFS.
  
  
  METHODS
  FCFS were isolated by collagenase II and purified with differential attachment and detachment method. The proliferation of FCFS after ALD administration was assessed by CCK-8. The mRNA expression of COL1A1 and COL3A1 were assessed by reverse transcription polymerase chain reaction (RT-PCR) and the protein production of COL1A1 and COL3A1 were assessed by Western blot.
  
  
  RESULTS
  ALD facilitated the proliferation of FCFS concentration-dependently. ALD with lower concentration (10(-9);, 10(-8);, 10(-7); mol/L) significantly improved the expression of COL1A1 and COL3A1, while ALD with higher concentration of had no obvious effect.
  
  
  CONCLUSIONS
  ALD improved the proliferation of FCFS concentration-dependently. And in a certain concentration range, ALD improved the expression of COL1A1 and COL3A1 while higher concentration had opposite effect. There is no linear relationship among the effects of ALD on the proliferation of FCFS, expression and protein production of COL1A1 and COL3A1.
authors.affiliation: Department of Cardiology, Chinese PLA General Hospital, Beijing 100853, China. xieyongjin19820206@yahoo.cn
authors.forename: Yong-jin
authors.initials: YJ
authors.lastname: Xie
authors.forename: Sheng-qi
authors.initials: SQ
authors.lastname: Wang
authors.forename: Jin-song
authors.initials: JS
authors.lastname: Chen
authors.forename: Xiao-chen
authors.initials: XC
authors.lastname: Bo
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.forename: Yi-hong
authors.initials: YH
authors.lastname: Ren
mesh_terms: D000450:Aldosterone / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D003094:Collagen / Q000096:biosynthesis* / Q000235:genetics; D005318:Fetal Heart / Q000166:cytology / Q000187:drug effects*; D005347:Fibroblasts / Q000187:drug effects* / Q000502:physiology; D005355:Fibrosis; D006801:Humans; D009206:Myocardium / Q000473:pathology; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D012333:RNA, Messenger; D000450:Aldosterone; D003094:Collagen