title: K+ efflux is required for histone H3 dephosphorylation by Listeria monocytogenes listeriolysin O and other pore-forming toxins.
abstract: Chromatin modification triggered by bacteria is a newly described mechanism by which pathogens impact host transcription. Listeria monocytogenes dephosphorylates histone H3 through the action of listeriolysin O (LLO); however, the underlying mechanism is unknown. Here we show that an unrelated pore-forming toxin, Aeromonas aerolysin, also provokes H3 dephosphorylation (dePH3). As reported for aerolysin, we show that LLO and related toxins induce a pore-dependent K(+) efflux and that this efflux is the signal required for dePH3. In addition, LLO-induced K(+) efflux activates caspase-1. However, we demonstrate that dePH3 is unlinked to this activation. Therefore, our study unveils K(+) efflux as an important signal leading to two independent events critical for infection, inflammasome activation and histone modification.
authors.affiliation: Institut Pasteur, Unité des Interactions Bactéries-Cellules, Paris F-75015, France.
authors.forename: Mélanie Anne
authors.initials: MA
authors.lastname: Hamon
authors.forename: Pascale
authors.initials: P
authors.lastname: Cossart
mesh_terms: D001427:Bacterial Toxins / Q000235:genetics / Q000378:metabolism*; D020170:Caspase 1 / Q000378:metabolism; D045744:Cell Line, Tumor; D002784:Cholesterol / Q000494:pharmacology; D002843:Chromatin / Q000378:metabolism; D003603:Cytotoxins / Q000378:metabolism; D006367:HeLa Cells; D006360:Heat-Shock Proteins / Q000378:metabolism*; D006460:Hemolysin Proteins / Q000378:metabolism*; D006657:Histones / Q000378:metabolism*; D006801:Humans; D015151:Immunoblotting; D058847:Inflammasomes / Q000378:metabolism; D008089:Listeria monocytogenes / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity; D010766:Phosphorylation; D052899:Pore Forming Cytotoxic Proteins / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D015398:Signal Transduction
chemical_list: D001427:Bacterial Toxins; D002843:Chromatin; D003603:Cytotoxins; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; D006657:Histones; D058847:Inflammasomes; D052899:Pore Forming Cytotoxic Proteins; C032676:aerolysin; D002784:Cholesterol; D020170:Caspase 1; C038705:hlyA protein, Listeria monocytogenes; D011188:Potassium