title: Lack of association of polymorphic variants of genes encoding zinc transporters with the risk of orofacial cleft-affected pregnancies.
abstract: Maternal zinc deficiency seems to be a risk factor for orofacial clefts in offspring. This study was undertaken to investigate the involvement of polymorphic variants of genes for zinc transporters in the susceptibility of clefting. PCRRFLP analysis was used to analyze single nucleotide polymorphisms of SLC30A1 (rs7526700, rs2278651, rs611386), SLC30A4 (rs2453531, rs8029246), SLC30A5 (rs351444, rs164393, rs6886492), SLC39A1 (rs10127484, rs11264736), and SLC39A3 (rs759071, rs4806874, rs10415622) in mothers of children with non-syndromic cleft lip with or without cleft palate (CL/P) and control mothers. The allele, genotype, and haplotype distribution was found to be similar among case and control mothers. Also, the gene-by-gene interaction analysis conducted using the Multifactor Dimensionality Reduction approach revealed no significant interactive genetic effect on having a child with a cleft. In conclusion, our results demonstrated that the analyzed polymorphic variants of genes for zinc transporters are not implicated in abnormal palatogenesis in the investigated group of women from the Polish population.
authors.affiliation: Department of Paediatrics, Institute of Mother and Child, Warsaw, Poland. khozyasz@verco.com.pl
authors.forename: Kamil K
authors.initials: KK
authors.lastname: Hozyasz
authors.forename: Adrianna
authors.initials: A
authors.lastname: Mostowska
authors.forename: Margarita
authors.initials: M
authors.lastname: Lianeri
authors.forename: Barbara
authors.initials: B
authors.lastname: Offert
authors.forename: Pawel P
authors.initials: PP
authors.lastname: Jagodzinski
mesh_terms: D000328:Adult; D027682:Cation Transport Proteins / Q000235:genetics*; D002971:Cleft Lip / Q000235:genetics*; D002972:Cleft Palate / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D015032:Zinc / Q000378:metabolism*
chemical_list: D027682:Cation Transport Proteins; C491597:SLC30A4 protein, human; C457225:SLC30A5 protein, human; C403517:SLC39A1 protein, human; C106542:SLC39A3 protein, human; D015032:Zinc