title: A new strategy for the discrimination of mitochondrial DNA haplogroups in Han population.
abstract: Mitochondrial DNA (mtDNA) haplogroup discrimination is interesting not only for phylogenetic and clinical but also for forensic studies. We discriminated the mtDNA haplogroups of 570 healthy unrelated Han people from Zhejiang Province, Southeast China, by comprehensive analysis mutations of the hypervariable segments-I sequence and diagnostic polymorphisms in mtDNA coding region using real-time polymerase chain reaction (RT-PCR), which was compared with the widely used PCR and restriction fragment length polymorphism (PCR-RFLP) method. The results showed that in superhaplogroup M, haplogroup D was the most common haplotype within this assay to 24.6%, and in the other superhaplotype N, haplogroup B and F were the most common groups. Samples re-identified by PCR-RFLP showed the consistent results that were got with RT-PCR. In conclusion, the RT-PCR strategy appears to be an accurate, reproducible, and sensitive technique for the discrimination of mtDNA haplogroups, especially for mass screenings quickly and economically.
authors.affiliation: Kidney Disease Center, First Affiliated Hospital, College of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou 310003, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Yang
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
authors.forename: Qiang
authors.initials: Q
authors.lastname: He
authors.forename: Yiling
authors.initials: Y
authors.lastname: Zhu
authors.forename: Xianghong
authors.initials: X
authors.lastname: Yang
authors.forename: Rong
authors.initials: R
authors.lastname: Lv
authors.forename: Jianghua
authors.initials: J
authors.lastname: Chen
mesh_terms: D044466:Asian People / Q000235:genetics; D002681:China; D022801:Complementarity Determining Regions / Q000235:genetics; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000235:genetics*; D005006:Ethnicity / Q000235:genetics*; D005828:Genetics, Population; D006239:Haplotypes; D006801:Humans; D009154:Mutation; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide
chemical_list: D022801:Complementarity Determining Regions; D017931:DNA Primers; D004272:DNA, Mitochondrial; D015345:Oligonucleotide Probes