title: New insights on the Chagas disease main vector Triatoma infestans (Reduviidae, Triatominae) brought by the genetic analysis of Bolivian sylvatic populations.
abstract: Triatoma infestans is the main and most widespread vector of Chagas disease in South America. For the first time, a large sample of sylvatic populations of T. infestans was analyzed by ITS-2 and mtCytB sequencing. ITS-2 showed a low level of polymorphism but revealed a dichotomy between the Andean and non-Andean sylvatic populations. On the contrary, mtCytB sequences showed a high polymorphism (19 haplotypes determined by 35 variable sites) revealing a strong structuring between most of the sylvatic populations and possible ancient isolation and bottleneck in the Northern Andes. The dichotomy Andean vs. non-Andean populations was not observed with this marker. Moreover, mtCytB haplotype genealogies showed that the non-Andean haplotypes would have derived from the Andean ones, supporting somewhat an Andean origin of the species. Nevertheless, a non-Andean origin could not be discarded because a remarkable genetic diversity was found in the non-Andean sample. The comparison of the sylvatic haplotypes with the domestic ones from GenBank suggested multiple events of T. infestans domestication in Andean and non-Andean areas, instead of a major and unique domestication event in the Bolivian Andes, as previously proposed.
authors.affiliation: MIVEGEC (Maladies Infectieuses et Vecteurs, Ecologie, Génétique, Evolution et Contrôle, IRD 224 - CNRS 5290 - Université Montpellier 1 - Université Montpellier 2, Institut de Recherche pour le Développement, 911 Avenue Agropolis, BP 64501, 34394 Montpellier, France. etienne.waleckx@ird.fr
authors.forename: Etienne
authors.initials: E
authors.lastname: Waleckx
authors.forename: Renata
authors.initials: R
authors.lastname: Salas
authors.forename: Nerida
authors.initials: N
authors.lastname: Huamán
authors.forename: Rosio
authors.initials: R
authors.lastname: Buitrago
authors.forename: Marie-France
authors.initials: MF
authors.lastname: Bosseno
authors.forename: Claudia
authors.initials: C
authors.lastname: Aliaga
authors.forename: Christian
authors.initials: C
authors.lastname: Barnabé
authors.forename: Roberto
authors.initials: R
authors.lastname: Rodriguez
authors.forename: Faustine
authors.initials: F
authors.lastname: Zoveda
authors.forename: Marcelo
authors.initials: M
authors.lastname: Monje
authors.forename: Marianne
authors.initials: M
authors.lastname: Baune
authors.forename: Sergio
authors.initials: S
authors.lastname: Quisberth
authors.forename: Erick
authors.initials: E
authors.lastname: Villena
authors.forename: Pierre
authors.initials: P
authors.lastname: Kengne
authors.forename: François
authors.initials: F
authors.lastname: Noireau
authors.forename: Simone Frédérique
authors.initials: SF
authors.lastname: Brenière
mesh_terms: D000818:Animals; D001483:Base Sequence; D001838:Bolivia / Q000453:epidemiology; D014355:Chagas Disease / Q000453:epidemiology / Q000635:transmission*; D045303:Cytochromes b; D004247:DNA / Q000235:genetics; D021901:DNA, Intergenic; D003710:Demography; D014644:Genetic Variation; D006801:Humans; D008928:Mitochondria; D008969:Molecular Sequence Data; D010802:Phylogeny; D014224:Triatoma / Q000235:genetics* / Q000502:physiology
chemical_list: D021901:DNA, Intergenic; D004247:DNA; D045303:Cytochromes b