title: Small RNA library preparation for next-generation sequencing by single ligation, extension and circularization technology.
abstract: The discovery of novel small RNA classes and species has accelerated since the implementation of high-throughput sequencing technologies for the identification of small RNAs. However, as the sequence coverage increases in a cell, the expectation of finding novel small RNAs from a batch of sequencing gradually decreases. To improve the finding of novel small RNAs, an alternative small RNA library preparation method, the single ligation, extension and circularization method, has been developed which is adequate for high throughput sequencing. The procedure is faster and simpler than the more widely used procedures, and the constructed libraries are compatible with high-level multiplex analysis. The analysis of human small RNA libraries prepared by the SLEC method reported known small RNAs and novel small RNAs including 25 mirtron candidates. This study demonstrates that the method is effective in identifying known and novel small RNAs.
authors.affiliation: Department of Bioscience & Biotechnology, Sejong University, 98 Kunja-dong, Kwangjin-gu, Seoul, Republic of Korea. yngskwon@sejong.ac.kr
authors.forename: Young-Soo
authors.initials: YS
authors.lastname: Kwon
mesh_terms: D001483:Base Sequence; D045744:Cell Line, Tumor; D003001:Cloning, Molecular / Q000379:methods*; D030561:Databases, Nucleic Acid; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D035683:MicroRNAs / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D010316:Particle Size; D015203:Reproducibility of Results; D017423:Sequence Analysis, RNA
chemical_list: D035683:MicroRNAs