title: [Inhibitory effect of solanine on prostate cancer cell line PC-3 in vitro].
abstract: OBJECTIVE
  To investigate the mechanisms of the effects of solanine on human androgen-independent prostate cancer cell line PC-3 in vitro.
  
  
  METHODS
  PC-3 cells were treated with solanine at the concentration of 0, 30, 40 and 50 microg/ml, and the cell activity was measured by CCK-8 at 12, 24 and 48 hours after the treatment. At 24 hours, the cell cycle and apoptosis were detected by flow cytometry and fluorescence microscopy, and the protein expressions of I(kappa)B(alpha) and Bcl-2 determined by Western blot.
  
  
  RESULTS
  Solanine suppressed the growth of PC-3 cells in a dose- and time-dependent manner in vitro, with significant differences among different concentration and time groups (P < 0.05). The cycle of the PC-3 cells was arrested in the S phase (P < 0.05), with a significantly higher rate of apoptosis in the treated groups than in the controls (P < 0.05). The protein expression of I(kappa)B(alpha) was obviously up-regulated and that of Bcl-2 down-regulated in all the solanine concentration groups.
  
  
  CONCLUSIONS
  Solanine has an anti-prostate cancer effect by inhibiting PC-3 cell proliferation, arresting the S phase, inducing cell apoptosis, up-regulating the protein expression of I(kappa)B(alpha) and down-regulating that of Bcl-2.
authors.affiliation: Department of Urology, The 5th People's Hospital of Shanghai, Fudan University, Shanghai 200240, China. med_zj@sina.com
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.forename: Guo-wei
authors.initials: GW
authors.lastname: Shi
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D020672:I-kappa B Proteins / Q000378:metabolism; D008297:Male; D000072000:NF-KappaB Inhibitor alpha; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D012992:Solanine / Q000494:pharmacology*
chemical_list: D020672:I-kappa B Proteins; C000606204:NFKBIA protein, human; D019253:Proto-Oncogene Proteins c-bcl-2; D000072000:NF-KappaB Inhibitor alpha; D012992:Solanine