title: Picobirnavirus detection in bovine and buffalo calves from foothills of Himalaya and Central India.
abstract: The present study describes detection of picobirnavirus (PBV) in faecal samples from bovine and buffalo calves employing the polyacrylamide gel electrophoresis (PAGE). A total of 136 faecal samples from buffalo (n = 122) and cow calves (n = 14) exhibiting clinical signs of diarrhoea and from healthy calves were collected during 2007-2010 from subtropical (central India) and tarai area of western temperate Himalayan foothills (Uttarakhand). The dsRNA nature of the virus was confirmed by nuclease treatment (RNase A, RNaseT1 and DNase 1). PAGE results confirmed 3.67% (5/136) positivity for PBV, showing a typical genomic migration pattern with two discrete bands with size of approximately 2.4 and 1.7 kbps for the larger and smaller segments, respectively. Among the five PBV samples identified, three were from buffalo calves and one from cow calf exhibiting clinical signs of acute diarrhoea, while one sample from non-diarrhoeic buffalo calf also showed the presence of PBV. None of the samples showed dual infection of rotavirus and PBV. The preliminary findings indicate sporadic incidences of PBV in bovine calves and emphasize the need for the development of better diagnostics for early detection and genetic characterization of these emerging isolates of farm animals of economic significance.
authors.affiliation: Division of Virology, Indian Veterinary Research Institute, Mukteswar, Nainital, Uttarakhand 263138, India. malikyps@yahoo.com
authors.forename: Yashpal Singh
authors.initials: YS
authors.lastname: Malik
authors.forename: K M
authors.initials: KM
authors.lastname: Chandrashekar
authors.forename: Kuldeep
authors.initials: K
authors.lastname: Sharma
authors.forename: Adil A
authors.initials: AA
authors.lastname: Haq
authors.forename: Nirupama
authors.initials: N
authors.lastname: Vaid
authors.forename: Somendu
authors.initials: S
authors.lastname: Chakravarti
authors.forename: Munish
authors.initials: M
authors.lastname: Batra
authors.forename: Rashmi
authors.initials: R
authors.lastname: Singh
authors.forename: A B
authors.initials: AB
authors.lastname: Pandey
mesh_terms: D000818:Animals; D002020:Buffaloes; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000821:virology; D003612:Dairying; D003967:Diarrhea / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D005243:Feces / Q000382:microbiology*; D015994:Incidence; D007194:India / Q000453:epidemiology; D018708:Picobirnavirus / Q000235:genetics / Q000302:isolation & purification*; D012327:RNA Virus Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D012330:RNA, Double-Stranded / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D012401:Rotavirus / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000453:epidemiology / Q000662:veterinary / Q000821:virology
chemical_list: D012330:RNA, Double-Stranded; D012367:RNA, Viral