title: The CD133+ tumor stem-like cell-associated antigen may elicit highly intense immune responses against human malignant glioma.
abstract: To explore the immunogenicity of glioma stem-like cell-associated antigens (SAAs) from sorted or unsorted glioma tumor stem-like cells (TSCs) as well as irradiated TSCs. Two primary human malignant glioma lines (SHG62, SHG66) and U87 cell line were primarily cultured in the serum-free medium (SFM) supplemented with EGF/bFGF. TSCs were identified by their self-renewal, multi-lineage differentiation and tumorigenic activity. To prepare SAAs in vitro, CD133+ TSCs were sorted either by magnetic cell sorting or with irradiation (6 Gy).The cytotoxicity induced by autogenous myeloid dendritic cell (DC)-mediated SAA-specific cytotoxic T lymphocytes (CTLs) was assessed by the Just Another Method test. SHG62, SHG66, and U87 cells contained TSCs. CD133+ SAAs-specific CTLs were significantly more cytotoxic than effector cells loaded with unsorted SAA (P < 0.05). Effector cells loaded with irradiated SAAs were more cytotoxic than those with regular SAAs (P < 0.01). SAAs from CD133+ TSCs and irradiated TSCs provide highly immunogenic antigens. TSCs might be a novel source of antigens for DC vaccination against malignant gliomas.
authors.affiliation: Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, 200040, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Hua
authors.forename: Yu
authors.initials: Y
authors.lastname: Yao
authors.forename: Yiwei
authors.initials: Y
authors.lastname: Chu
authors.forename: Ping
authors.initials: P
authors.lastname: Zhong
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Sheng
authors.forename: Baoguo
authors.initials: B
authors.lastname: Xiao
authors.forename: Jingsong
authors.initials: J
authors.lastname: Wu
authors.forename: Bojie
authors.initials: B
authors.lastname: Yang
authors.forename: Ying
authors.initials: Y
authors.lastname: Mao
authors.forename: Liangfu
authors.initials: L
authors.lastname: Zhou
mesh_terms: D000071916:AC133 Antigen; D000818:Animals; D015703:Antigens, CD / Q000276:immunology* / Q000378:metabolism; D001932:Brain Neoplasms / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D002470:Cell Survival; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D005260:Female; D005434:Flow Cytometry; D005910:Glioma / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D006023:Glycoproteins / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008875:Middle Aged; D014411:Neoplastic Stem Cells / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D010455:Peptides / Q000276:immunology* / Q000378:metabolism; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D014407:Tumor Cells, Cultured
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides; C000606024:Prom1 protein, mouse