title: Leucobacter exalbidus sp. nov., an actinobacterium isolated from a mixed culture from compost.
abstract: A gram-positive bacterium, strain K-540B(T), which was separated from a mixed culture isolated from compost, was subjected to a polyphasic taxonomic study. Strain K-540B(T) was found to have 95.5%-98.7% sequence similarity with the genus Leucobacter based on 16S rRNA gene sequence analyis. Phenotypic and chemical properties of strain K-540B(T) were consistent with its classification in the genus Leucobacter. The major quinone system compound was MK-11 and major fatty acids were anteiso-C(15:0) and iso-C(16:0). Polar lipids included phosphatidylglycerol and diphosphatidylglycerol. The G+C content of the genomic DNA was 64.9 mol%. The cell wall peptidoglycan contained diaminobutyric acid and γ-aminobutyric acid. DNA-DNA hybridization results and physiological tests genotypically and phenotypically differentiated strain K-540B(T) from other Leucobacter species. Based on these results, strain K-540B(T) (=NBRC 106062(T)=DSM 22850(T)), for which the name Leucobacter exalbidus sp. nov. is proposed, represents a novel species of the genus Leucobacter.
authors.affiliation: Medicinal Chemistry Research Laboratories, Kyowa Hakko Kirin Co., Ltd., Machida-shi, Tokyo, Japan. harumi.ue@kyowa-kirin.co.jp
authors.forename: Harumi
authors.initials: H
authors.lastname: Ue
mesh_terms: D000192:Actinomycetales / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques / Q000379:methods*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D008055:Lipids / Q000096:biosynthesis; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D008055:Lipids; D012336:RNA, Ribosomal, 16S