title: An investigation on the approach to target lipopolysaccharide through polymeric capped nano-structured formulation for the management of sepsis.
abstract: A ciprofloxacin (CFn)-loaded lipid emulsion was developed for treatment of intra-abdominal infections, especially sepsis. Loading efficiency depended on the proportion of chitosan (CH) and sodium deoxycholate (SDC). The average globule size was 225 to 325 nm. Animal survival improved when the prototype formulation was administered to LPS-induced septic mice. At 4 microg/ml, reduction in TNF-alpha and NO production were observed (P < 0.05) but at lower concentration these changes were not significant (P > 0.05) as compared to positive control (LPS-1 microg/ml). This indicates that chitosan can modify LPS interaction with macrophages, and that formulations have potential to control inflammation associated with sepsis.
authors.affiliation: Pharmaceutics Division, Central Drug Research Institute, (Council of Scientific and Industrial Research, India), P.O. Box 173, M.G. Marg, Lucknow 226001, India.
authors.forename: P R
authors.initials: PR
authors.lastname: Mishra
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000008:administration & dosage / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry*; D002939:Ciprofloxacin / Q000008:administration & dosage* / Q000737:chemistry; D003840:Deoxycholic Acid / Q000737:chemistry*; D004339:Drug Compounding / Q000379:methods; D008070:Lipopolysaccharides; D051379:Mice; D008807:Mice, Inbred BALB C; D053769:Nanocapsules / Q000737:chemistry*; D018805:Sepsis / Q000139:chemically induced* / Q000473:pathology / Q000517:prevention & control*; D016896:Treatment Outcome
chemical_list: D000890:Anti-Infective Agents; D008070:Lipopolysaccharides; D053769:Nanocapsules; D003840:Deoxycholic Acid; D002939:Ciprofloxacin; D048271:Chitosan