title: Tapping an unexploited repository: Carnoy's fixed cell pellets for proteomic biomarker research in leukemia.
abstract: For chromosomal analysis in tumor genetics, cells from blood and bone marrow are prepared and preserved virtually indefinitely in Carnoy's fixative (methanol/acetic acid). Numerous samples are stored unvalued in hospitals and institutes worldwide. We developed a method to analyze proteins from even a small amount of these cells by mass spectrometry using affinity chromatographic surfaces (SELDI), and demonstrated the application of proteomic biomarker research in cases of acute myeloid leukemia.
authors.affiliation: Core Unit Chip Application, Institute of Human Genetics and Anthropology, Klinikum der FSU Jena, D-07740 Jena, Germany. fegg@mti.uni-jena.de.
authors.forename: Ferdinand
authors.initials: F
authors.lastname: Von Eggeling
authors.forename: Annett
authors.initials: A
authors.lastname: Bleul
authors.forename: Ivan F
authors.initials: IF
authors.lastname: Loncarevic
authors.forename: Susanne
authors.initials: S
authors.lastname: Michel
authors.forename: Anita
authors.initials: A
authors.lastname: Heller
authors.forename: Christian
authors.initials: C
authors.lastname: Melle