title: Phenol glycosides with in vitro anti-Helicobacter pylori activity from Hypericum erectum Thunb.
abstract: To evaluate the anti-Helicobacter pylori activity of Hypericum erectum methanol extracts in order to provide the primary evidence for their use in clinical practice. An ethyl acetate fraction of H. erectum suspension-cell cultures inhibited the growth of H. pylori in vitro, with a MIC50 range of 38.7-63.2 μg/mL, comparable to metronidazole (MIC50  = 43.2 μg/mL). To further investigate the involved active compounds of the H. erectum extracts, four phenol glycosides were isolated for the current study: quercetin-3'-O-β-D-galactopyranoside, quercetin-3'-O-(2''-acetyl)-β-D-glucopyranoside, 4,6-dihydroxy-2-methoxyphenyl-1-O-β-D-glucopyranoside and 4-hydroxy-2,6-dimethoxyphenyl-1-O-α-L-rhamnopyranosyl(1-6)-β-D-glucopyranoside. The MIC50 values of 4,6-dihydroxy-2-methoxyphenyl-1-O-β-D-glucopyranoside and 4-hydroxy-2,6-dimethoxyphenyl-1-O-α-L-rhamnopyranosyl(1-6)-β-D-glucopyranoside from ATCC43504 strains were 7.3 and 27.3 μg/mL, respectively. The other two phenol glycosides did not show anti-H. pylori activity. The results of this work suggest that H. erectum has some therapeutic potential against H. pylori infection, which could be explored for patients with gastroduodenal disorders.
authors.affiliation: Department of Medicinal Biotechnology, College of Natural Resources and Life Science, Dong-A University, Busan, 604-714, South Korea. himun68@gmail.com.
authors.forename: Hyung-In
authors.initials: HI
authors.lastname: Moon
authors.forename: Young-Choon
authors.initials: YC
authors.lastname: Lee
authors.forename: Jai-Heon
authors.initials: JH
authors.lastname: Lee
mesh_terms: D005697:Galactosides / Q000494:pharmacology; D006027:Glycosides / Q000494:pharmacology*; D016480:Helicobacter pylori / Q000187:drug effects*; D020902:Hypericum / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D010636:Phenols / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology*; D011794:Quercetin / Q000031:analogs & derivatives / Q000494:pharmacology; D056910:Republic of Korea
chemical_list: D005697:Galactosides; D006027:Glycosides; D010636:Phenols; D010936:Plant Extracts; C508165:quercetin-3-O-beta-D-galactopyranoside; D011794:Quercetin