title: Proteomic analysis of the differentially expressed proteins by airborne nanoparticles.
abstract: Airborne nanoparticles with thermodynamic diameters less than 56 nm (PM(0.056)) were collected using a Moudi cascade impactor, and the differentially expressed proteins upon exposure to the airborne nanoparticles were identified in human bronchial epithelial cells. More than 600 protein spots were detected on the two-dimensional gel, and the identified 13 of these proteins showed notable changes. Nine were up-regulated and four were down-regulated following treatment with the airborne nanoparticles. Notably, malignant transformation-associated multiple forms of keratins, epigenetic regulation-related MBD1-containing chromatin associated factor 2, epithelial malignancy-related vimentin and exocytosis-related annexin A2 were changed upon exposure to airborne nanoparticle PM(0.056).
authors.affiliation: Department of Medical Biotechnology, SoonChunHyang University, Asan, Chungnam, 336-600, Republic of Korea.
authors.forename: Seul Ki
authors.initials: SK
authors.lastname: Park
authors.forename: Yu Mi
authors.initials: YM
authors.lastname: Jeon
authors.forename: Bu Soon
authors.initials: BS
authors.lastname: Son
authors.forename: Hyung Sun
authors.initials: HS
authors.lastname: Youn
authors.forename: Mi Young
authors.initials: MY
authors.lastname: Lee
mesh_terms: D000393:Air Pollutants / Q000737:chemistry / Q000633:toxicity*; D017306:Annexin A2 / Q000235:genetics / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D015153:Blotting, Western; D001980:Bronchi / Q000187:drug effects* / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007633:Keratins / Q000235:genetics / Q000378:metabolism; D019216:Metals, Heavy / Q000032:analysis; D053758:Nanoparticles / Q000737:chemistry / Q000633:toxicity*; D040901:Proteomics; D020545:Respiratory Mucosa / Q000187:drug effects* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014746:Vimentin / Q000235:genetics / Q000378:metabolism
chemical_list: C472822:ANXA2 protein, human; D000393:Air Pollutants; D017306:Annexin A2; D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; C066141:MBD1 protein, human; D019216:Metals, Heavy; D014157:Transcription Factors; D014746:Vimentin; D007633:Keratins