title: High transfection efficiency of neural stem cells with magnetofection.
abstract: Primary neural stem cells (NSCs) can be cultivated and differentiated in vitro but are difficult to transfect using conventional methods. We describe a simple and rapid magnetofection-based method suitable for the lab bench as well as for high-throughput projects. Our method yields high transfection efficiency and can be used for deciphering the genetic control of neural cell differentiation.
authors.affiliation: OZ Biosciences, Marseille, France. csapet@ozbiosciences.com
authors.forename: Cédric
authors.initials: C
authors.lastname: Sapet
authors.forename: Nicolas
authors.initials: N
authors.lastname: Laurent
authors.forename: Antoine
authors.initials: A
authors.lastname: de Chevigny
authors.forename: Loïc
authors.initials: L
authors.lastname: Le Gourrierec
authors.forename: Elodie
authors.initials: E
authors.lastname: Bertosio
authors.forename: Olivier
authors.initials: O
authors.lastname: Zelphati
authors.forename: Christophe
authors.initials: C
authors.lastname: Béclin
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004247:DNA / Q000008:administration & dosage*; D008280:Magnetics; D051379:Mice; D058953:Neural Stem Cells / Q000166:cytology*; D055495:Neurogenesis; D014162:Transfection / Q000191:economics / Q000379:methods*
chemical_list: D004247:DNA