title: Cell surface engineering with polyelectrolyte multilayer thin films.
abstract: Layer-by-layer assembly of polyelectrolyte multilayer (PEM) films represents a bottom-up approach for re-engineering the molecular landscape of cell surfaces with spatially continuous and molecularly uniform ultrathin films. However, fabricating PEMs on viable cells has proven challenging owing to the high cytotoxicity of polycations. Here, we report the rational engineering of a new class of PEMs with modular biological functionality and tunable physicochemical properties which have been engineered to abrogate cytotoxicity. Specifically, we have discovered a subset of cationic copolymers that undergoes a conformational change, which mitigates membrane disruption and facilitates the deposition of PEMs on cell surfaces that are tailorable in composition, reactivity, thickness, and mechanical properties. Furthermore, we demonstrate the first successful in vivo application of PEM-engineered cells, which maintained viability and function upon transplantation and were used as carriers for in vivo delivery of PEMs containing biomolecular payloads. This new class of polymeric film and the design strategies developed herein establish an enabling technology for cell transplantation and other therapies based on engineered cells.
authors.affiliation: Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332, USA.
authors.forename: John T
authors.initials: JT
authors.lastname: Wilson
authors.forename: Wanxing
authors.initials: W
authors.lastname: Cui
authors.forename: Veronika
authors.initials: V
authors.lastname: Kozlovskaya
authors.forename: Eugenia
authors.initials: E
authors.lastname: Kharlampieva
authors.forename: Di
authors.initials: D
authors.lastname: Pan
authors.forename: Zheng
authors.initials: Z
authors.lastname: Qu
authors.forename: Venkata R
authors.initials: VR
authors.lastname: Krishnamurthy
authors.forename: Joseph
authors.initials: J
authors.lastname: Mets
authors.forename: Vivek
authors.initials: V
authors.lastname: Kumar
authors.forename: Jing
authors.initials: J
authors.lastname: Wen
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Song
authors.forename: Vladimir V
authors.initials: VV
authors.lastname: Tsukruk
authors.forename: Elliot L
authors.initials: EL
authors.lastname: Chaikof
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity; D057005:Bioengineering; D002462:Cell Membrane / Q000737:chemistry*; D004573:Electrolytes / Q000138:chemical synthesis / Q000737:chemistry / Q000633:toxicity; D007515:Islets of Langerhans / Q000737:chemistry; D016381:Islets of Langerhans Transplantation; D051379:Mice; D011073:Polyamines / Q000138:chemical synthesis / Q000737:chemistry / Q000633:toxicity; D000071228:Polyelectrolytes; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry / Q000633:toxicity; D011107:Polylysine / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000737:chemistry / Q000633:toxicity; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity
chemical_list: D001672:Biocompatible Materials; D004573:Electrolytes; D011073:Polyamines; D000071228:Polyelectrolytes; D011092:Polyethylene Glycols; D011107:Polylysine; D011108:Polymers; C443714:polylysine-graft-(poly(ethylene glycol))