title: Dimerisation process of silybin-type flavonolignans: insights from theory.
abstract: Natural polyphenols are known to be oxidized by free radicals, which partially explains the antioxidant properties of a number of these compounds. This oxidation may also be used to synthesise new compounds of biological interest, for example, dimers. The present theoretical study describes the existing experimental evidence showing that silybin and dehydrosilybin [natural polyphenols isolated from milk thistle (Silybum marianum)] form dimers regioselectively. Based on DFT calculations, thermodynamic and kinetic values were computed in order to better understand the physicochemical behaviour of these dimerisation processes. Calculations showed that after H-atom transfer (from polyphenol to radical), dimerisation could proceed in two steps: 1) bond formation and, when possible, 2) tautomerisation reorganisation. The former step is the limiting step, while the latter is crucial for the process to be thermodynamically favourable (ΔG<0). If this rearrangement is impossible then dimerisation is not feasible, or at least becomes a minor process (e.g., dehydrosilybin dimerisation after H-atom abstraction from the 3-OH group). This explains the regioselectivity of polyphenol dimerisation.
authors.affiliation: EA4021, Université de Limoges, Limoges, France.
authors.forename: Pavlína
authors.initials: P
authors.lastname: Košinová
authors.forename: Radek
authors.initials: R
authors.lastname: Gažák
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Duroux
authors.forename: Roberto
authors.initials: R
authors.lastname: Lazzaroni
authors.forename: Vladimír
authors.initials: V
authors.lastname: Křen
authors.forename: Xavier
authors.initials: X
authors.lastname: Assfeld
authors.forename: Patrick
authors.initials: P
authors.lastname: Trouillas
mesh_terms: D019281:Dimerization; D044947:Flavonolignans / Q000737:chemistry*; D007700:Kinetics; D010084:Oxidation-Reduction; D059808:Polyphenols / Q000737:chemistry; D000077385:Silybin; D012838:Silymarin / Q000737:chemistry*; D013237:Stereoisomerism; D013816:Thermodynamics
chemical_list: D044947:Flavonolignans; D059808:Polyphenols; D012838:Silymarin; D000077385:Silybin