title: Identification of a phylogenetically distinct orthobunyavirus from group C.
abstract: Apeu virus (APEUV) (family Bunyaviridae, genus Orthobunyavirus) was plaque purified and characterised by serological and molecular analysis. Neutralising assays confirmed cross-reactivity between purified APEUV clones and the Caraparu virus complex of group C orthobunyaviruses. Partial sequencing of the L, M and S segments of one APEUV clone (APEUV-CL5) was carried out. A phylogenetic tree constructed with the L amino acid sequences clustered APEUV-CL5 within the genus Orthobunyavirus, confirming its serological classification. Analysis of M segment sequences clustered APEUV-CL5 in the Caraparu virus complex (Group C), in agreement with serological tests and previous molecular characterisation. However, the sequence of the nucleocapsid gene (N) gave low identity values when compared to those of the group C viruses. The phylogenetic tree based on N nucleotide sequences clustered APEUV-CL5 next to the California and Bwamba groups. This remarkable S nucleotide variability suggests that APEUV-CL5 could be a genetic reassortant and that this evolutionary mechanism is present in the history of the group C viruses.
authors.affiliation: Laboratório de Bioquímica Metabólica, Departamento de Ciências Biológicas, Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil. cintia.magalhaes@gmail.com
authors.forename: Cintia Lopes
authors.initials: CL
authors.lastname: de Brito Magalhães
authors.forename: Betânia Paiva
authors.initials: BP
authors.lastname: Drumond
authors.forename: Renata Franco Vianna
authors.initials: RF
authors.lastname: Novaes
authors.forename: Bárbara Rezende
authors.initials: BR
authors.lastname: Quinan
authors.forename: José Carlos
authors.initials: JC
authors.lastname: de Magalhães
authors.forename: João Rodrigues
authors.initials: JR
authors.lastname: dos Santos
authors.forename: Carla do Amaral
authors.initials: Cdo A
authors.lastname: Pinto
authors.forename: Marieta Torres de Abreu
authors.initials: MT
authors.lastname: Assis
authors.forename: Cláudio Antônio
authors.initials: CA
authors.lastname: Bonjardim
authors.forename: Erna Geessien
authors.initials: EG
authors.lastname: Kroon
authors.forename: Paulo César Peregrino
authors.initials: PC
authors.lastname: Ferreira
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D002044:Bunyaviridae Infections / Q000821:virology*; D002460:Cell Line; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D016851:Orthobunyavirus / Q000145:classification* / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D010802:Phylogeny
chemical_list: D000914:Antibodies, Viral