title: Biologic response to autogenous particulate bone graft and shaped titanium mesh in segmental mandibular defect reconstruction.
abstract: OBJECTIVE
  This study evaluated the biologic response to titanium mesh and autogenous particulate bone grafts for the reconstruction of segmental mandibular defects in a dog model.
  
  
  METHODS
  Unilateral 40-mm critical-sized continuity defects of the mandible were made in five beagle dogs. Titanium mesh was shaped and fixed with titanium screws in the defects. Autogenous bone was harvested from the ablated mandible and iliac crest (the ratio of cortical bone:cancellous bone was 3:1) and used to fill the mesh. Two implants were placed into each bone graft. The animals were sacrificed after 6 months. Radiographs, histologic sections, scanning electron microscopy, and energy dispersive spectroscopy were performed to evaluate bone formation and osseointegration of the implants in the reconstructed mandibles.
  
  
  RESULTS
  The outline of the reconstructed mandible was satisfactory, and no bone resorption was observed in the defect area. All implants showed excellent osseointegration of the grafted bone. Furthermore, the density of bone formed around the implants was higher than that seen in control samples (intact, ungrafted dog hemimandibles).
  
  
  CONCLUSIONS
  Shaped titanium mesh with autogenous particulate bone graft is a predictable method for restoring critical-sized continuity defects of the mandible. Simultaneous implant placement is feasible and the structure of bone formed near implants may be optimal.
authors.affiliation: Research Institute of Stomatology, Nanjing Medical University, Jiangsu, China. danxiangren2006@163.com
authors.forename: Ning
authors.initials: N
authors.lastname: Chen
authors.forename: Yi-fei
authors.initials: YF
authors.lastname: Du
authors.forename: Ji-lai
authors.initials: JL
authors.lastname: Guo
authors.forename: Shuang-yue
authors.initials: SY
authors.lastname: Zhang
authors.forename: Jiang-feng
authors.initials: JF
authors.lastname: Tao
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D015519:Bone Density / Q000502:physiology; D001863:Bone Screws; D016025:Bone Transplantation / Q000000981:diagnostic imaging / Q000379:methods*; D003758:Dental Implantation, Endosseous; D015921:Dental Implants; D004195:Disease Models, Animal; D004285:Dogs; D005240:Feasibility Studies; D005452:Fluoresceins; D005456:Fluorescent Dyes; D008336:Mandibular Diseases / Q000000981:diagnostic imaging / Q000601:surgery*; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D016348:Osseointegration / Q000502:physiology; D010012:Osteogenesis / Q000502:physiology; D011859:Radiography; D019651:Plastic Surgery Procedures / Q000295:instrumentation / Q000379:methods*; D013052:Spectrometry, X-Ray Emission; D013526:Surgical Mesh; D020858:Tissue and Organ Harvesting / Q000379:methods; D014025:Titanium / Q000737:chemistry*; D014182:Transplantation, Autologous
chemical_list: D001672:Biocompatible Materials; D015921:Dental Implants; D005452:Fluoresceins; D005456:Fluorescent Dyes; D014025:Titanium; C007740:fluorexon