title: Proteomic analysis of lipopolysaccharide-treated submandibular gland in rat.
abstract: We investigated changes in the protein profile of submandibular gland (SMG) with inflammation induced by exposure to lipopolysaccharide (LPS) with the aim of identifying potential molecular markers of injured gland. Lipopolysaccharide (2.5µg) was directly administered into rat SMG unilaterally by retrograde ductal injection. At 12hr after treatment, the gland was excised and the proteins identified by two-dimensional difference gel electrophoresis and matrix-assisted laser desorption/ionization-time of flight mass spectrometry. Many proteins in the LPS-treated gland showed a marked change compared to those in the contralateral gland. Of particular note were increases in ubiquitin, a highly-conserved small regulatory protein and in calgranulin B, which has an immunological function in inflammation. Proteins related to apoptosis and stress also showed change in the inflamed gland. The results of this study suggest that the ubiquitin system of protein modification is involved in LPS-induced inflammation in salivary gland, and that a number of specific proteins might be applicable as molecular markers in the monitoring of inflamed or injured gland.
authors.affiliation: Department of Pharmacology, Tokyo Dental College, Miham-ku, Chiba, Japan. sawaki@tdc.ac.jp
authors.forename: Kohei
authors.initials: K
authors.lastname: Sawaki
authors.forename: Takashi
authors.initials: T
authors.lastname: Shinomiya
authors.forename: Migiwa
authors.initials: M
authors.lastname: Okubo
authors.forename: Eri
authors.initials: E
authors.lastname: Tsukagoshi
authors.forename: Makoto
authors.initials: M
authors.lastname: Ogane
authors.forename: Mototsugu
authors.initials: M
authors.lastname: Matsuura
authors.forename: Masanobu
authors.initials: M
authors.lastname: Yoshikawa
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Kawaguchi
mesh_terms: D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000096:biosynthesis; D040502:Calgranulin B / Q000096:biosynthesis*; D015536:Down-Regulation; D006360:Heat-Shock Proteins / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000494:pharmacology; D008291:Malate Dehydrogenase / Q000096:biosynthesis; D008297:Male; D020543:Proteome / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012793:Sialadenitis / Q000276:immunology / Q000378:metabolism / Q000382:microbiology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013363:Submandibular Gland / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D057825:Two-Dimensional Difference Gel Electrophoresis; D025801:Ubiquitin / Q000096:biosynthesis*; D015854:Up-Regulation
chemical_list: D051017:Apoptosis Regulatory Proteins; D040502:Calgranulin B; D006360:Heat-Shock Proteins; D008070:Lipopolysaccharides; D020543:Proteome; C489790:Stip1 protein, rat; D025801:Ubiquitin; D008291:Malate Dehydrogenase