title: Selecting temperature for protein crystallization screens using the temperature dependence of the second virial coefficient.
abstract: Protein crystals usually grow at a preferable temperature which is however not known for a new protein. This paper reports a new approach for determination of favorable crystallization temperature, which can be adopted to facilitate the crystallization screening process. By taking advantage of the correlation between the temperature dependence of the second virial coefficient (B(22)) and the solubility of protein, we measured the temperature dependence of B(22) to predict the temperature dependence of the solubility. Using information about solubility versus temperature, a preferred crystallization temperature can be proposed. If B(22) is a positive function of the temperature, a lower crystallization temperature is recommended; if B(22) shows opposite behavior with respect to the temperature, a higher crystallization temperature is preferred. Otherwise, any temperature in the tested range can be used.
authors.affiliation: Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an, Shaanxi, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.forename: Da-Chuan
authors.initials: DC
authors.lastname: Yin
authors.forename: Yun-Zhu
authors.initials: YZ
authors.lastname: Guo
authors.forename: Xi-Kai
authors.initials: XK
authors.lastname: Wang
authors.forename: Si-Xiao
authors.initials: SX
authors.lastname: Xie
authors.forename: Qin-Qin
authors.initials: QQ
authors.lastname: Lu
authors.forename: Yong-Ming
authors.initials: YM
authors.lastname: Liu
mesh_terms: D000818:Animals; D002645:Chickens; D002919:Chymotrypsinogen / Q000737:chemistry; D003460:Crystallization / Q000379:methods*; D008027:Light; D008956:Models, Chemical; D009113:Muramidase / Q000737:chemistry; D010316:Particle Size; D011506:Proteins / Q000737:chemistry*; D012031:Refractometry; D015203:Reproducibility of Results; D012542:Scattering, Radiation; D012996:Solutions; D013696:Temperature
chemical_list: D011506:Proteins; D012996:Solutions; D002919:Chymotrypsinogen; C421864:hen egg lysozyme; D009113:Muramidase