title: Importance of viral genomic composition in modulating glycoprotein content on the surface of influenza virus particles.
abstract: Despite progress in our knowledge of the internal organisation of influenza virus particles, little is known about the determinants of their morphology and, more particularly, of the actual abundance of structural proteins at the virion level. To address these issues, we used cryo-EM to focus on viral (and host) factors that might account for observed differences in virion morphology and characteristics such as size, shape and glycoprotein (GP) spike density. Twelve recombinant viruses were characterised in terms of their morphology, neuraminidase activity and virus growth. The genomic composition was shown to be important in determining the GP spike density. In particular, polymerase gene segments and especially PB1/PB2 were shown to have a prominent influence in addition to that for HA in determining GP spike density, a feature consistent with a functional link between these virus components important for virus fitness.
authors.affiliation: Université Lyon 1, Faculté de médecine RTH Laennec, CNRS FRE 3011 VirPath, Virologie et Pathologie Humaine, F-69008 Lyon, France. vincent.moules@univ-lyon1.fr
authors.forename: Vincent
authors.initials: V
authors.lastname: Moulès
authors.forename: Olivier
authors.initials: O
authors.lastname: Terrier
authors.forename: Matthieu
authors.initials: M
authors.lastname: Yver
authors.forename: Beatrice
authors.initials: B
authors.lastname: Riteau
authors.forename: Christine
authors.initials: C
authors.lastname: Moriscot
authors.forename: Olivier
authors.initials: O
authors.lastname: Ferraris
authors.forename: Thomas
authors.initials: T
authors.lastname: Julien
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Giudice
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Rolland
authors.forename: Alexandra
authors.initials: A
authors.lastname: Erny
authors.forename: Maude
authors.initials: M
authors.lastname: Bouscambert-Duchamp
authors.forename: Emilie
authors.initials: E
authors.lastname: Frobert
authors.forename: Manuel
authors.initials: M
authors.lastname: Rosa-Calatrava
authors.forename: Yi
authors.initials: Y
authors.lastname: Pu Lin
authors.forename: Alan
authors.initials: A
authors.lastname: Hay
authors.forename: Daniel
authors.initials: D
authors.lastname: Thomas
authors.forename: Guy
authors.initials: G
authors.lastname: Schoehn
authors.forename: Bruno
authors.initials: B
authors.lastname: Lina
mesh_terms: D000818:Animals; D002460:Cell Line; D020285:Cryoelectron Microscopy; D004285:Dogs; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000378:metabolism* / Q000648:ultrastructure; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000378:metabolism / Q000648:ultrastructure*; D053122:Influenza A Virus, H3N2 Subtype / Q000378:metabolism / Q000648:ultrastructure*; D009439:Neuraminidase / Q000378:metabolism* / Q000648:ultrastructure; D012324:RNA-Dependent RNA Polymerase / Q000378:metabolism; D014764:Viral Proteins / Q000378:metabolism* / Q000648:ultrastructure; D014771:Virion / Q000378:metabolism / Q000648:ultrastructure*
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus; C057027:PB2 protein, Influenzavirus A; D014764:Viral Proteins; C077346:influenza virus polymerase basic protein 1; D012324:RNA-Dependent RNA Polymerase; C487630:NA protein, influenza A virus; D009439:Neuraminidase