title: Analysis of genet size and local gene flow in the ectomycorrhizal basidiomycete Suillus spraguei (synonym S. pictus).
abstract: Several recent fine-scale genetic structure studies of ectomycorrhizal fungi have reported significant spatial clustering of genets with similar genotypes, supporting locally restricted gene flow. In this study we used genotype data from microsatellite markers and spatial autocorrelation analysis to examine local gene flow in Suillus spraguei at distances up to 2 km. Previously developed microsatellite markers for S. spraguei from Japan were unsuccessful at amplifying DNA isolated from sporocarps found in New York state, and other research suggested that both are disjunct species. Novel microsatellite markers therefore were developed with New York specimens. We identified nine polymorphic microsatellite loci and developed primer sets to amplify these regions. We tested the efficiency of the primer sets on 50 sporocarps collected from a natural Pinus strobus stand. The majority of the markers were in Hardy-Weinberg and linkage equilibrium. The location of all sampled sporocarps was recorded and used along with multilocus genotype data to create a genet map. The distance between sporocarps with the same multilocus genotype was small (≤ 7.65 m) and the majority of sporocarps collected were genetically unique, suggesting frequent spore establishment and sexual recombination on this site. Spatial autocorrelation analysis did not support clustering of similar genotypes, suggesting few restrictions to gene flow within this local population.
authors.affiliation: State University of New York College of Environmental Science and Forestry, Department of Environmental and Forest Biology, 1 Forestry Drive, Syracuse, New York 13210, USA.
authors.forename: Kathleen M
authors.initials: KM
authors.lastname: Burchhardt
authors.forename: Yazmin
authors.initials: Y
authors.lastname: Rivera
authors.forename: Thomas
authors.initials: T
authors.lastname: Baldwin
authors.forename: David
authors.initials: D
authors.lastname: Vanearden
authors.forename: Annette M
authors.initials: AM
authors.lastname: Kretzer
mesh_terms: D001483:Base Sequence / Q000235:genetics; D001487:Basidiomycota / Q000235:genetics*; D017931:DNA Primers; D051456:Gene Flow; D005819:Genetic Markers / Q000235:genetics; D005828:Genetics, Population / Q000379:methods; D007564:Japan; D018895:Microsatellite Repeats / Q000235:genetics; D008969:Molecular Sequence Data; D038821:Mycorrhizae / Q000235:genetics*; D009518:New York; D028223:Pinus / Q000382:microbiology; D018517:Plant Roots / Q000382:microbiology; D011110:Polymorphism, Genetic
chemical_list: D017931:DNA Primers; D005819:Genetic Markers