title: [Identification of six kinds of monoclonal antibodies against rat Nogo-A molecule in the immunofluorescent histochemical staining].
abstract: OBJECTIVE
  To detect and characterize of 6 monoclonal antibodies (mAbs) against different epitopes of rat Nogo-A molecule in immunohistochemistry to decide their applications in futrue. Four mAbs against Nogo66 fragment are named Nogo66-1, Nogo66-2, Nogo66-3 and Nogo66-4. The rest of 2 mAbs against N-termial 570-691aa fragment are named NogoN-1 and NogoN-2.
  
  
  METHODS
  The immunofluorescence staining was used to detect the reactivity and specificity of those 6 mAbs in spinal tissue sections of rat.
  
  
  RESULTS
  All 6 mAbs were double-labelled with commercial rabbit anti-Rat Nogo-A polyclonal antibody (PcAb) in spinal cord sections respecitvely. All 6 mAbs were colocalization with MBP respectively. However Nogo66-3 and NogoN-1 could also be double-staining with GFAP respectively.
  
  
  CONCLUSIONS
  Nogo66-1, Nogo66-2, Nogo66-4 and NogoN-2 could recognize specifically in Nogo-A protein of tissues in immunohistochemical methods.
authors.affiliation: Department of Neurobiology, Fourth Military Medical University, Xi'an 710032, China. xajxdb@126.com
authors.forename: Bin
authors.initials: B
authors.lastname: Deng
authors.forename: Fang-fang
authors.initials: FF
authors.lastname: Liu
authors.forename: Xiang-hui
authors.initials: XH
authors.lastname: Zhao
authors.forename: Cai-yong
authors.initials: CY
authors.lastname: Yu
authors.forename: Gan-lan
authors.initials: GL
authors.lastname: Bian
authors.forename: Rui
authors.initials: R
authors.lastname: Feng
authors.forename: Gong
authors.initials: G
authors.lastname: Ju
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D009185:Myelin Proteins / Q000032:analysis / Q000276:immunology*; D000070798:Nogo Proteins; D051381:Rats
chemical_list: D000911:Antibodies, Monoclonal; D009185:Myelin Proteins; D000070798:Nogo Proteins; C000603201:Rtn4 protein, mouse; C000603197:Rtn4 protein, rat