title: Cryopreservation of umbilical cord blood-derived mesenchymal stem cells without dimethyl sulfoxide.
abstract: Cryopreservation of umbilical cord blood-derived mesenchymal stem cells (UCB-derived MSCs) is crucial step for its clinical applications in cell transplantation therapy. In the cryopreservation of MSCs, dimethyl sulfoxide has been widely used as a cryoprotectant (CPA). However, it has been proved that DMSO has toxic side effects to human body. In this study, DMSO-free CPA solutions which contained ethylene glycol (EG), 1, 2-propylene glycol (PG) and sucrose as basic CPAs, supplemented with polyvinyl alcohol (PVA) as an additive, were developed for the cryopreservation of UCB-derived MSCs. The cryopreservation of UCB-derived MSCs was achieved by vitrification via plunging into liquid nitrogen and by programmed freezing via an optical-DSC system respectively. The viability of thawed UCB-derived MSCs was tested by trypan blue exclusion assay. Results showed that the viability of thawed UCB-derived MSCs was enhanced from 71.2% to 95.4% in the presence of PVA for vitrification, but only < 10% to 45% of viability was found for programmed freezing. These results indicate that PVA exerts a beneficial effect on the cryopreservation of UCB-derived MSCs and suggest the vitrification in combination with the dimethyl sulfoxide free CPA solutions supplemented with PVA would be an efficient protocol for the cryopreservation of UCB-derived MSCs.
authors.affiliation: School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou, China.
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Wang
authors.forename: Zhao-Rong
authors.initials: ZR
authors.lastname: Lun
authors.forename: Shu-Shen
authors.initials: SS
authors.lastname: Lu
mesh_terms: D002470:Cell Survival; D015925:Cryopreservation; D003451:Cryoprotective Agents / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000633:toxicity; D019855:Ethylene Glycol / Q000494:pharmacology; D005260:Female; D005312:Fetal Blood / Q000166:cytology* / Q000187:drug effects; D005333:Fetus; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D011142:Polyvinyl Alcohol / Q000494:pharmacology; D019946:Propylene Glycol / Q000494:pharmacology; D013395:Sucrose / Q000494:pharmacology; D014343:Trypan Blue; D058989:Vitrification / Q000187:drug effects
chemical_list: D003451:Cryoprotective Agents; D013395:Sucrose; D019946:Propylene Glycol; D011142:Polyvinyl Alcohol; D019855:Ethylene Glycol; D014343:Trypan Blue; D004121:Dimethyl Sulfoxide