title: Real-time four-dimensional optical-resolution photoacoustic microscopy with Au nanoparticle-assisted subdiffraction-limit resolution.
abstract: Photoacoustic microscopy (PAM) offers label-free, optical absorption contrast. A high-speed, high-resolution PAM system in an inverted microscope configuration with a laser pulse repetition rate of 100,000 Hz and a stationary ultrasonic transducer was built. Four-dimensional in vivo imaging of microcirculation in mouse skin was achieved at 18 three-dimensional volumes per second with repeated two-dimensional (2D) raster scans of 100 by 50 points. The corresponding 2D B-scan (50 A-lines) frame rate was 1800 Hz, and the one-dimensional A-scan rate was 90,000 Hz. The lateral resolution is 0.23 ± 0.03 μm for Au nanowire imaging, which is 2.0 times below the diffraction limit.
authors.affiliation: Department of Biomedical Engineering, Optical Imaging Lab, Washington University in St. Louis, Campus Box 1097, One Brookings Drive, St. Louis, Missouri 63130-4899, USA.
authors.forename: Bin
authors.initials: B
authors.lastname: Rao
authors.forename: Konstantin
authors.initials: K
authors.lastname: Maslov
authors.forename: Amos
authors.initials: A
authors.lastname: Danielli
authors.forename: Ruimin
authors.initials: R
authors.lastname: Chen
authors.forename: K Kirk
authors.initials: KK
authors.lastname: Shung
authors.forename: Qifa
authors.initials: Q
authors.lastname: Zhou
authors.forename: Lihong V
authors.initials: LV
authors.lastname: Wang
mesh_terms: D000162:Acoustics; D000818:Animals; D004423:Ear; D004912:Erythrocytes / Q000166:cytology; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*; D055620:Optical Phenomena; D012867:Skin / Q000166:cytology; D013997:Time Factors
chemical_list: D006046:Gold