title: In vivo intra-articular negative pressure wound therapy effect on cartilage in a goat model.
abstract: This prospective, randomized, blinded pilot study determined if a difference was present in the histology and apoptotic rate of articular cartilage after application of a negative pressure wound therapy (NPWT) device to an uninjured joint surface compared to a control side using Capra hircus goats. The goats were euthanized at 3 or 7 days after surgery. The en bloc joint resection was divided into medial (direct sponge contact) and lateral compartments (no sponge contact; indirect NPWT). In the necropsied cartilage and menisci, there were no gross or histologic/morphometric differences identified by a blinded veterinary pathologist. The percentages of apoptotic and necrotic chondrocytes based on flow cytometry were not statistically different. This study demonstrated that there were no observable deleterious effects to uninjured cartilage from direct or indirect intra-articular NPWT placement. These data suggest that NPWT may be placed safely in an intra-articular position for up to 7 days. Further studies in humans are warranted.
authors.affiliation: 2200 Bergquist Drive, Suite 1, SGOYO, Orthopaedics, Lackland AFB, TX 78236, USA. Kenneth.bode@us.af.mil
authors.forename: Kenneth
authors.initials: K
authors.lastname: Bode
authors.forename: Charles
authors.initials: C
authors.lastname: Haggerty
authors.forename: J T
authors.initials: JT
authors.lastname: Tokish
authors.forename: Warren
authors.initials: W
authors.lastname: Kadrmas
mesh_terms: D000818:Animals; D017209:Apoptosis; D002358:Cartilage, Articular / Q000473:pathology; D019902:Chondrocytes / Q000473:pathology; D005434:Flow Cytometry; D006041:Goats; D023421:Models, Animal; D054843:Negative-Pressure Wound Therapy; D011446:Prospective Studies; D011897:Random Allocation