title: Concentration-dependent fluorescence live-cell imaging and tracking of intracellular nanoparticles.
abstract: Using live-cell imaging techniques we investigated concentration-dependent intracellular movements of fluorescence nanoparticles (NPs) in real-time after their entry into HeLa cells via incubation. Intracellular particle traces appeared to be a mixture of both random and fairly unidirectional movements of the particles. At rather low concentrations of NPs, a majority of the non-random intracellular particle trajectories are assumed to mostly go along microtubule networks after endocytosis, as evidenced from the inhibition test with nocodazole. On the other hand, as the concentrations of NPs increased, random motions were more frequently observed inside the cells.
authors.affiliation: Department of Chemistry, Soongsil University, Seoul, Korea.
authors.forename: Ji Hye
authors.initials: JH
authors.lastname: Seo
authors.forename: Keunchang
authors.initials: K
authors.lastname: Cho
authors.forename: So Yeong
authors.initials: SY
authors.lastname: Lee
authors.forename: Sang-Woo
authors.initials: SW
authors.lastname: Joo
mesh_terms: D002470:Cell Survival; D004705:Endocytosis; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods; D053758:Nanoparticles / Q000032:analysis*