title: A prognostic multigene classifier for squamous cell carcinomas of the larynx.
abstract: Survival after diagnosis of laryngeal cancer has not improved over the last 20 years. Selection of patients for radio- and chemotherapy or surgery or follow-up strategies based on a prognostic classifier could improve survival without unduly extending radical surgery. We performed microarray gene expression analysis and developed a four-gene classifier for laryngeal cancer using Prediction Analysis of Microarray and leave-one-out cross validation. A four-gene classifier containing the non-coding gene H19, the histone HIST1H3F and the two small nucleolar RNAs, SNORA16A and SNORD14C was developed that assigns cases to low and high risk classes. The high risk class has a relative risk of 6.5 (CI=1.817-23.258, Fisher exact test p<0.0001). The maternally imprinted gene H19 is the top classifier gene.
authors.affiliation: Integrated Molecular Pathology, National Cancer Research Institute, Genova, Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Mirisola
authors.affiliation: ENT Department, University of Genova, Italy.
authors.forename: Renzo
authors.initials: R
authors.lastname: Mora
authors.affiliation: Integrated Molecular Pathology, National Cancer Research Institute, Genova, Italy.
authors.forename: Alessia Isabella
authors.initials: AI
authors.lastname: Esposito
authors.affiliation: ENT Department, University of Genova, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Guastini
authors.affiliation: Integrated Molecular Pathology, National Cancer Research Institute, Genova, Italy.
authors.forename: Flavia
authors.initials: F
authors.lastname: Tabacchiera
authors.affiliation: Integrated Molecular Pathology, National Cancer Research Institute, Genova, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Paleari
authors.affiliation: Integrated Molecular Pathology, National Cancer Research Institute, Genova, Italy.
authors.forename: Adriana
authors.initials: A
authors.lastname: Amaro
authors.affiliation: Integrated Molecular Pathology, National Cancer Research Institute, Genova, Italy.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Angelini
authors.affiliation: ENT Department, University of Genova, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Dellepiane
authors.affiliation: Integrated Molecular Pathology, National Cancer Research Institute, Genova, Italy. Electronic address: ulrich.pfeffer@istge.it.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Pfeffer
authors.affiliation: ENT Department, University of Genova, Italy.
authors.forename: Angelo
authors.initials: A
authors.lastname: Salami
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000145:classification* / Q000235:genetics* / Q000601:surgery; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007822:Laryngeal Neoplasms / Q000145:classification* / Q000235:genetics* / Q000601:surgery; D008297:Male; D035683:MicroRNAs / Q000502:physiology; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000235:genetics; D009367:Neoplasm Staging; D020411:Oligonucleotide Array Sequence Analysis; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs; D012333:RNA, Messenger