title: [Genetic characterization of wild-type mumps virus isolated in Liaoning Province].
abstract: Three mumps virus strains were isolated using Vero/Slam cell line from the patients' throat swabs and urines during mumps outbreaks and sporadic period in Liaoning province from 2008. Fragments of 1028 nucleotides including SH genes from 3 mumps virus isolates were amplified by RT-PCR, the PCR products were ligated into pMD19-T vector and cloned to JM109 cell. By blue-white selection, the positive white clones were sequenced and analyzed. Based on the 316 nucleotides of SH gene, the phylogenetic analyses were processed with WHO mumps reference strains downloaded from GenBank and 3 mumps viruses strains. It wan shown that the 3 mumps virus strains isolated in 2008 belonged to F genotype, 3 strains (LN-2008-001-06, LN-2008-001-07 and LN-2008-001-10) showed a nucleotide and amino acid similarity of 98.7%-100% and 94.7%-100% respectively. Two strains (LN-2008-001-06 and LN-2008-001-10) had the same sequence completely. Comparing to the F reference strains, the 3 mumps virus strains' nucleotide and amino acid similarity of 92.4%-96.2% and 84.2%-94.7% respectively. Due to the limited strain numbers, whether the F genotype was the predominant circulating genotype can not be determined. The surveillance on the mumps virus in Liaoning should be therefore strengthened.
authors.affiliation: Liaoning Provincial Center for Disease Control and Prevention, Shenyang 110005, China. dawangyan@lncdc.com
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Ma
authors.forename: Yue
authors.initials: Y
authors.lastname: Han
authors.forename: Jun-qiao
authors.initials: JQ
authors.lastname: Guo
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002681:China / Q000453:epidemiology; D006801:Humans; D008969:Molecular Sequence Data; D009107:Mumps / Q000453:epidemiology / Q000821:virology*; D009109:Mumps virus / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D010802:Phylogeny; D016415:Sequence Alignment; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D014764:Viral Proteins