title: Evaluation of lytic activity of staphylococcal bacteriophage Sb-1 against freshly isolated clinical pathogens.
abstract: In recent decades the increase in antibiotic-resistant bacterial strains has become a serious threat to the treatment of infectious diseases. Drug resistance of Staphylococcus aureus has become a major problem in hospitals of many countries, including developed ones. Today the interest in alternative remedies to antibiotics, including bacteriophage treatment, is gaining new ground. Here, we describe the staphylococcal bacteriophage Sb-1 - a key component of therapeutic phage preparation that was successfully used against staphylococcal infections during many years in the Former Soviet Union. This phage still reveals a high spectrum of lytic activity in vitro against freshly isolated, genetically different clinical samples (including methicillin-resistant S. aureus) obtained from the local hospitals, as well as the clinics from different geographical areas. The sequence analyses of phage genome showed absence of bacterial virulence genes. A case report describes a promising clinical response after phage application in patient with cystic fibrosis and indicates the efficacy of usage of Sb-1 phage against various staphylococcal infections.
authors.affiliation: Laboratory of Genetic Engineering and Biotechnology, G Eliava Institute of Bacteriophages, Microbiology and Virology, 3, Gotua str, Tbilisi 0160, Georgia.
authors.forename: Leila
authors.initials: L
authors.lastname: Kvachadze
authors.forename: Nana
authors.initials: N
authors.lastname: Balarjishvili
authors.forename: Tamila
authors.initials: T
authors.lastname: Meskhi
authors.forename: Ekaterine
authors.initials: E
authors.lastname: Tevdoradze
authors.forename: Natia
authors.initials: N
authors.lastname: Skhirtladze
authors.forename: Tamila
authors.initials: T
authors.lastname: Pataridze
authors.forename: Revaz
authors.initials: R
authors.lastname: Adamia
authors.forename: Temur
authors.initials: T
authors.lastname: Topuria
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Kutter
authors.forename: Christine
authors.initials: C
authors.lastname: Rohde
authors.forename: Mzia
authors.initials: M
authors.lastname: Kutateladze
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001483:Base Sequence; D001691:Biological Therapy / Q000379:methods*; D002648:Child; D024881:Drug Resistance, Bacterial; D005260:Female; D016679:Genome, Viral; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D013203:Staphylococcal Infections / Q000382:microbiology* / Q000628:therapy; D013204:Staphylococcus Phages / Q000235:genetics / Q000502:physiology*; D013211:Staphylococcus aureus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D014764:Viral Proteins