title: The effect of coimmobilizing heparin and fibronectin on titanium on hemocompatibility and endothelialization.
abstract: Currently available cardiovascular implants, such as heart valves and stents, exhibit suboptimal biocompatibility because of the incomplete endothelialization and sequential thrombosis formation especially after a long-term implantation. To improve the blood compatibility and endothelialization simultaneously and ensure the long-term effect of the cardiovascular implants, a technique of combining electrostatic interaction and coimmobilization was developed to form heparin and fibronectin (Hep/Fn) films on aminosilanized titanium (Ti) surfaces. The Hep/Fn coimmobilized films were stable after immersion in PBS for five days, probed by wettability studies and by the release kinetics of heparin and fibronectin. Blood compatibility tests showed that the coimmobilized Hep/Fn films displayed lower hemolysis rate, prolonged blood coagulation time, higher AT III binding density, less platelets activation and aggregation, and less fibrinogen conformational change compared with Ti surface. Endothelial cells (ECs) seeding and fibronectin bioactivity results showed more attached and proliferated ECs and exposed cell-binding sites on the Hep/Fn immobilized samples than that on Ti surfaces. Thus, the Hep/Fn coimmobilized films kept excellent bioactivity even after immersion in PBS for five days. Systemic evaluation suggests that the coimmobilization of Hep/Fn complex improves the blood compatibility and promotes the endothelialization simultaneously. We envisage that this method will provide a potential and effective selection for biomaterials surface modification of cardiovascular implants.
authors.affiliation: Institute of Biomaterials and Surface Engineering, Key Lab. for Advanced Technologies of Materials, Ministry of Education, Southwest Jiaotong University, Chengdu 610031, PR China.
authors.forename: Guicai
authors.initials: G
authors.lastname: Li
authors.forename: Ping
authors.initials: P
authors.lastname: Yang
authors.forename: Wei
authors.initials: W
authors.lastname: Qin
authors.forename: Manfred F
authors.initials: MF
authors.lastname: Maitz
authors.forename: Shuo
authors.initials: S
authors.lastname: Zhou
authors.forename: Nan
authors.initials: N
authors.lastname: Huang
mesh_terms: D000818:Animals; D000990:Antithrombin III / Q000378:metabolism; D049109:Cell Proliferation; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D042783:Endothelial Cells / Q000166:cytology / Q000502:physiology*; D005353:Fibronectins / Q000737:chemistry* / Q000378:metabolism; D006493:Heparin / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D008422:Materials Testing; D018625:Microscopy, Atomic Force; D056951:Photoelectron Spectroscopy; D015539:Platelet Activation; D010973:Platelet Adhesiveness; D012844:Sincalide / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D005353:Fibronectins; D000990:Antithrombin III; D006493:Heparin; D014025:Titanium; D012844:Sincalide