title: Effect of cross-fostering on transfer of maternal immunity to Mycoplasma hyopneumoniae to piglets.
abstract: The aim of this study was to assess the effect of cross-fostering on transfer of maternal Mycoplasma hyopneumoniae-specific humoral and cell-mediated immunity (CMI) from gilts to piglets. Cross-fostering, carried out within gilt pairs, was based on the gilts' M hyopneumoniae vaccination status in accordance with the following scheme: six pairs of vaccinated gilt × non-vaccinated gilt (V × N); five pairs of non-vaccinated gilt × vaccinated gilt (N × V); and five pairs of vaccinated gilt × vaccinated gilt (V × V). The piglets were cross-fostered at 0, six, 12 or 20 hours after birth. Two piglets per gilt per time point were cross-fostered (that is, eight piglets per gilt were moved) and the remaining piglets served as non-cross-fostered controls. In addition, four litters served as non-cross-fostered controls. A maximum of 10 piglets per gilt were sampled. The piglets' M hyopneumoniae-specific humoral immunity was assessed by ELISA and their CMI was assessed by delayed-type hypersensitivity testing. M hyopneumoniae-specific antibodies were detected in non-cross-fostered piglets from vaccinated dams and from piglets cross-fostered within the V × N gilt pair at six hours or more, and within the V × V gilt pair at all time points. Piglets cross-fostered within the N × V gilt pair had detectable M hyopneumoniae-specific antibodies only if they had been moved within six hours of birth. The transfer of M hyopneumoniae-specific CMI to piglets appeared to be source-dependent, and was detected only in piglets maintained on their vaccinated dams for at least 12 hours after birth.
authors.affiliation: Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, St Paul, MN 55108, USA.
authors.forename: M
authors.initials: M
authors.lastname: Bandrick
authors.forename: M
authors.initials: M
authors.lastname: Pieters
authors.forename: C
authors.initials: C
authors.lastname: Pijoan
authors.forename: S K
authors.initials: SK
authors.lastname: Baidoo
authors.forename: T W
authors.initials: TW
authors.lastname: Molitor
mesh_terms: D000818:Animals; D000833:Animals, Suckling; D000907:Antibodies, Bacterial / Q000097:blood; D001428:Bacterial Vaccines / Q000276:immunology*; D003126:Colostrum / Q000276:immunology*; D005260:Female; D007112:Immunity, Maternally-Acquired; D018445:Infectious Disease Transmission, Vertical / Q000517:prevention & control / Q000662:veterinary; D045705:Mycoplasma hyopneumoniae / Q000276:immunology; D045729:Pneumonia of Swine, Mycoplasmal / Q000276:immunology* / Q000517:prevention & control / Q000635:transmission; D011247:Pregnancy; D013552:Swine; D014611:Vaccination / Q000662:veterinary
chemical_list: D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines