title: Banana peel: a potential substrate for laccase production by Aspergillus fumigatus VkJ2.4.5 in solid-state fermentation.
abstract: In solid-state fermentation, among various solid supports evaluated, banana peel was found to be an ideal support and resulted into higher levels of laccase (6281.4 ± 63.60 U l(-1)) along with notable levels of manganese peroxidase production (1339.0 ± 131.23 U l(-1)) by Aspergillus fumigatus VkJ2.4.5. Maximum levels of laccase was achieved under derived conditions consisting of 80% of moisture level, 6 days of incubation period, 6% inoculum level, and an aeration level of 2.5 l min(-1). A column-tray bioreactor was designed to scale up and economize the enzyme production in three successive cycles of fermentation using the same fungal biomass. Thermal and pH stability profiles revealed that enzyme was stable up to 50°C and at varying pH range from 5-9 for up to 2 h. The apparent molecular weight of laccase was found to be 34 ± 1 kDa. MALDI-TOF/TOF analysis of the protein showed significant homology with maximum identity of 67% to other laccases reported in database.
authors.affiliation: Protein Engineering and Proteomics Group, Department of Chemistry, Biotechnology and Food Sciences, Norwegian University of Life Sciences, Ås, 1430, Norway.
authors.forename: V
authors.initials: V
authors.lastname: Vivekanand
authors.forename: Pallavi
authors.initials: P
authors.lastname: Dwivedi
authors.forename: Nidhi
authors.initials: N
authors.lastname: Pareek
authors.forename: Rajesh P
authors.initials: RP
authors.lastname: Singh
mesh_terms: D001232:Aspergillus fumigatus / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology; D005285:Fermentation / Q000502:physiology*; D042845:Laccase / Q000096:biosynthesis*; D028521:Musa / Q000737:chemistry*; D010544:Peroxidases / Q000378:metabolism
chemical_list: D042845:Laccase; D010544:Peroxidases; C051129:manganese peroxidase