title: Characterisation of a satellite RNA of Cucumber mosaic virus that induces chlorosis in Capsicum annuum.
abstract: The presence of Cucumber mosaic virus (CMV) satellite RNA dramatically changes symptoms on some hosts. A satellite RNA present in a strain of CMV (PepY-CMV) that induced chlorosis in pepper (Capsicum annuum) was shown to induce chlorosis in pepper in combination with another strain (Fny-CMV) that by itself induced a green mosaic symptom. The location of sequences within the PepY satellite RNA (PepY-satRNA) of CMV that conferred the ability to induce chlorosis on pepper plants were analyzed by exchanging sequence domains between cDNA clones of PepY-satRNA and an attenuated mosaic satellite RNA (Paf-satRNA), as well as site-directed mutagenesis of various clusters of the 22-nt sequence differences between the two satellite RNAs in the delimited central domain. The symptoms induced by site-directed mutants of PepY-satRNA and Paf-satRNA in the presence of Fny-CMV demonstrated an insertion within PepY-satRNA of 11 nt at positions 86-96 relative to Paf-satRNA determined the chlorosis-inducing phenotype. Within the chlorosis-inducing domain, deletion of nucleotides did not affect the satRNA replication but abolished the ability of PepY-satRNA to elicit chlorosis symptom. Conversely, a mutant satellite RNA derived from Paf-satRNA in which eleven nucleotides were inserted indicated that sequences of 11 nucleotides were found to be sufficient for chlorosis induction in pepper.
authors.affiliation: Virology Unit, Department of Horticultural Environment, National Institute of Horticultural & Herbal Science, RDA, Suwon, 441-440, Republic of Korea.
authors.forename: Seung-Kook
authors.initials: SK
authors.lastname: Choi
authors.forename: Yong-Woon
authors.initials: YW
authors.lastname: Jeon
authors.forename: Ju-Yeon
authors.initials: JY
authors.lastname: Yoon
authors.forename: Jang-Kyung
authors.initials: JK
authors.lastname: Choi
mesh_terms: D002212:Capsicum / Q000821:virology*; D017799:Cucumovirus / Q000235:genetics* / Q000472:pathogenicity*; D016254:Mutagenesis, Insertional; D016297:Mutagenesis, Site-Directed; D010935:Plant Diseases / Q000821:virology*; D019244:RNA, Satellite / Q000235:genetics*; D011995:Recombination, Genetic; D017384:Sequence Deletion; D037521:Virulence Factors / Q000235:genetics*; D014779:Virus Replication
chemical_list: D019244:RNA, Satellite; D037521:Virulence Factors