title: NSAIDs acutely inhibit TRPC channels in freshly isolated rat glomeruli.
abstract: Using a novel approach for analysis of TRPC channel activity, we report here that NSAIDs are involved into regulation of TRPC channels in the podocytes of the freshly isolated decapsulated glomeruli. Fluorescence and electron microscopy techniques confirmed the integrity of podocytes in the glomeruli. Western blotting showed that TRPC1, 3 and 6 are highly expressed in the glomeruli. Single-channel patch clamp analysis revealed cation currents with distinct TRPC properties. This is the first report describing single TRPC-like currents in glomerular podocytes. Furthermore, our data provide a novel mechanism of NSAIDs regulation of TRPC channels, which might be implicated in maintaining the glomerular filtration barrier.
authors.affiliation: Department of Physiology, Medical College of Wisconsin, Milwaukee, WI, USA.
authors.forename: Daria V
authors.initials: DV
authors.lastname: Ilatovskaya
authors.forename: Vladislav
authors.initials: V
authors.lastname: Levchenko
authors.forename: Robert P
authors.initials: RP
authors.lastname: Ryan
authors.forename: Allen W
authors.initials: AW
authors.lastname: Cowley
authors.forename: Alexander
authors.initials: A
authors.lastname: Staruschenko
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D004008:Diclofenac / Q000494:pharmacology; D005923:Glomerulosclerosis, Focal Segmental / Q000188:drug therapy / Q000378:metabolism; D007052:Ibuprofen / Q000494:pharmacology; D007678:Kidney Glomerulus / Q000187:drug effects*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D050052:TRPC Cation Channels / Q000037:antagonists & inhibitors* / Q000378:metabolism
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D050052:TRPC Cation Channels; D004008:Diclofenac; D007052:Ibuprofen