title: Altered expression of claudin-1, claudin-7, and tricellulin regardless of human papilloma virus infection in human tonsillar squamous cell carcinoma.
abstract: CONCLUSIONS
  Altered expression of claudin-1, claudin-7, and tricellulin in early tonsillar squamous cell carcinoma (SCC) independent of human papilloma virus (HPV) infection may lead to tumor progression.
  
  
  OBJECTIVE
  Integral tight junction proteins, the claudins and tricellulin, play a crucial role in all tissues. HPV is significantly associated with tonsillar SCC. We sought to determine the expression of claudin-1, claudin-7, and tricellulin in HPV-infected and HPV-free tonsillar SCC.
  
  
  METHODS
  Twenty-eight tonsillar SCCs were studied by immunohistochemical analysis and real-time reverse transcription polymerase chain reaction with in situ hybridization analysis.
  
  
  RESULTS
  Compared with normal tissues, claudin-1 was strongly expressed, whereas claudin-7 and tricellulin were weakly expressed or were absent in primary SCC and metastatic lymph nodes. Claudin-7 and tricellulin were markedly reduced at all stages of tumor development. In situ hybridization analysis showed no correlation between HPV infection and altered expression of the tight junction proteins.
authors.affiliation: Department of Otolaryngology, Sapporo Medical University School of Medicine, Chuo-ku, Sapporo, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kondoh
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Takano
authors.forename: Takashi
authors.initials: T
authors.lastname: Kojima
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Ohkuni
authors.forename: Ryuta
authors.initials: R
authors.lastname: Kamekura
authors.forename: Noriko
authors.initials: N
authors.lastname: Ogasawara
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Go
authors.forename: Norimasa
authors.initials: N
authors.lastname: Sawada
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Himi
mesh_terms: D014408:Biomarkers, Tumor / Q000096:biosynthesis / Q000235:genetics; D001706:Biopsy; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology / Q000821:virology; D062445:Claudin-1; D057167:Claudins / Q000096:biosynthesis / Q000235:genetics*; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D062794:MARVEL Domain Containing 2 Protein; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics*; D008875:Middle Aged; D027383:Papillomaviridae / Q000235:genetics; D030361:Papillomavirus Infections / Q000235:genetics* / Q000473:pathology / Q000821:virology; D012334:RNA, Neoplasm / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D012189:Retrospective Studies; D014067:Tonsillar Neoplasms / Q000235:genetics* / Q000473:pathology / Q000821:virology
chemical_list: D014408:Biomarkers, Tumor; C568108:CLDN1 protein, human; C473255:CLDN7 protein, human; D062445:Claudin-1; D057167:Claudins; D004279:DNA, Viral; D062794:MARVEL Domain Containing 2 Protein; C516798:MARVELD2 protein, human; D008565:Membrane Proteins; D012334:RNA, Neoplasm