title: IFN-γ enhances the anti-tumour immune response of dendritic cells against oral squamous cell carcinoma.
abstract: OBJECTIVE
  To investigate the expression of antigen processing-1 (Tap-1) and Tapasin in oral squamous cell carcinoma (OSCC), and observe the immune response against OSCC by use of IFN-γ-antigen induced dendritic cells (DCs) in vitro and in vivo.
  
  
  METHODS
  Expression of Tap-1 and Tapasin in different cell lines was analysed. CAL27 cells were treated with IFN-γ. Antigen from the treated cells was presented by DCs. Pulsed DC was then co-cultivated with CD8+ T lymphocyte to induce antigen specific cytotoxic lymphocytes (CTLs). The immune response elicited by CTLs against OSCC was observed.
  
  
  RESULTS
  A significant lower expression of Tap-1 and Tapasin was observed in OSCC cell lines. IFN-γ exerted time-dependent effect for increasing the expression of these genes. Antigen from the treated CAL27 cells was presented by DCs. CTLs were induced and generated a strong immune response in vitro and in vivo.
  
  
  CONCLUSIONS
  Tap-1 and Tapasin were downregulated in OSCC. IFN-γ increased the expression of these genes. Use of IFN-γ-antigen induced DCs could induce stronger immune response in vitro and in vivo.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Nanjing Stomatological Hospital, Affiliated to Medical School, Nanjing University, Nanjing 210008, China. doctorhanwei@hotmail.com
authors.forename: Han
authors.initials: H
authors.lastname: Wei
authors.forename: Pan
authors.initials: P
authors.lastname: Hongya
authors.forename: Jiang
authors.initials: J
authors.lastname: Linlin
authors.forename: Ali
authors.initials: A
authors.lastname: Mujiang
authors.forename: Wei
authors.initials: W
authors.lastname: Kuijie
authors.forename: Zou
authors.initials: Z
authors.lastname: Duohong
authors.forename: Hu
authors.initials: H
authors.lastname: Qingang
authors.forename: Zhang
authors.initials: Z
authors.lastname: Zhiyuan
mesh_terms: D000071181:ATP Binding Cassette Transporter, Subfamily B, Member 2; D018528:ATP-Binding Cassette Transporters / Q000096:biosynthesis / Q000235:genetics; D000818:Animals; D017951:Antigen Presentation; D002294:Carcinoma, Squamous Cell / Q000276:immunology*; D045744:Cell Line, Tumor / Q000187:drug effects; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology*; D000939:Epitopes; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology* / Q000494:pharmacology; D008213:Lymphocyte Activation; D026901:Membrane Transport Proteins / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D008819:Mice, Nude; D009062:Mouth Neoplasms / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
chemical_list: D000071181:ATP Binding Cassette Transporter, Subfamily B, Member 2; D018528:ATP-Binding Cassette Transporters; D000939:Epitopes; D026901:Membrane Transport Proteins; C073119:TAP1 protein, human; C107762:tapasin; D007371:Interferon-gamma