title: Anti-human FLT3 monoclonal antibody that inhibits proliferation of monocytic leukemia cell line SHI-1.
abstract: FLT3, a transmembrane molecule, was found on hematopoietic stem/progenitor cells and leukemia cells and determined to be a promising target in leukemia diagnosis and therapy. In this study a functional anti-human FLT3, monoclonal antibody (MAb) 10G6, was obtained and the specificity of this MAb was verified by flow cytometry. This MAb effectively recognized the FLT3 molecule expressed on a series of malignant cell lines. Furthermore, we demonstrated that MAb 10G6 inhibited the proliferation and migration ability and induced the apoptosis of SHI-1 cells that derived from a human monocytic leukemia. This functional anti-human FLT3 MAb provides a valuable tool for further study targeting the FLT3 on leukemia cells.
authors.affiliation: Biotechnology Institute, School of Medicine, Soochow University, Suzhou, China.
authors.forename: Songguang
authors.initials: S
authors.lastname: Ju
authors.forename: Zhimou
authors.initials: Z
authors.lastname: Xue
authors.forename: Songwen
authors.initials: S
authors.lastname: Ju
authors.forename: Yan
authors.initials: Y
authors.lastname: Ge
authors.forename: Wei
authors.initials: W
authors.lastname: Xie
authors.forename: Huanting
authors.initials: H
authors.lastname: Zhu
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Pan
authors.forename: Xueguang
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000494:pharmacology*; D000918:Antibody Specificity; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D005434:Flow Cytometry; D006801:Humans; D007938:Leukemia / Q000188:drug therapy* / Q000276:immunology; D009000:Monocytes / Q000187:drug effects / Q000502:physiology; D051941:fms-Like Tyrosine Kinase 3 / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; C497970:FLT3 protein, human; D051941:fms-Like Tyrosine Kinase 3