title: Distribution of serine/threonine kinase SAD-B in mouse peripheral nerve synapse.
abstract: The serine/threonine kinase SAD regulates neural functions such as axon/dendrite polarization and neurotransmitter release. In the vertebrate central nervous system, SAD-B, a homolog of Caenorhabditis elegans SAD-1, is associated with synaptic vesicles and the active zone cytomatrix in nerve terminals. However, the distribution of SAD-B in the peripheral nervous system remains elusive. Here, we show that SAD-B is specifically localized to neuromuscular junctions. Although the active zone protein bassoon showed a punctated signal indicating its localization to motor end plates, SAD-B shows relatively diffuse localization indicating its association with both the active zone and synaptic vesicles. Therefore, SAD kinase may regulate neurotransmitter release from motor end plates in a similar manner to its regulation of neurotransmitter release in the central nervous system.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, University of Yamanashi, Yamanashi, Japan.
authors.forename: Akari
authors.initials: A
authors.lastname: Hagiwara
authors.forename: Kenu
authors.initials: K
authors.lastname: Harada
authors.forename: Yamato
authors.initials: Y
authors.lastname: Hida
authors.forename: Isao
authors.initials: I
authors.lastname: Kitajima
authors.forename: Toshihisa
authors.initials: T
authors.lastname: Ohtsuka
mesh_terms: D000818:Animals; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009046:Motor Neurons / Q000166:cytology / Q000201:enzymology*; D009469:Neuromuscular Junction / Q000166:cytology / Q000201:enzymology*; D018377:Neurotransmitter Agents / Q000378:metabolism; D010525:Peripheral Nerves / Q000166:cytology / Q000201:enzymology*; D017729:Presynaptic Terminals / Q000201:enzymology*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D013569:Synapses / Q000201:enzymology*
chemical_list: D018377:Neurotransmitter Agents; C496826:Brsk1 protein, mouse; D017346:Protein Serine-Threonine Kinases