title: Polymorphisms in promoter regions of PDHB, SORBS1, and EDG1 genes showing marbling-associated expression changes.
abstract: We have recently showed that the pyruvate dehydrogenase (lipoamide) beta (PDHB) gene involved in fatty acid oxidation, the sorbin and SH3 domain containing 1 (SORBS1) gene involved in insulin-stimulated glucose uptake, and the endothelial differentiation, sphingolipid G-protein-coupled receptor, 1 (EDG1) gene involved in blood vessel formation possess expression differences in musculus longissimus muscle between low-marbled and high-marbled steer groups. In the present study, we detected single nucleotide polymorphisms (SNPs) in the promoter regions of the 3 genes between the 2 steer groups. A SNP in the EDG1 exhibited significantly different allelic distribution between animals with extremely high predicted breeding value for marbling and with extremely low one. The EDG1 SNP may be related to changes in gene expression and/or marbling.
authors.affiliation: Laboratory of Animal Breeding and Genetics, Graduate School of Agriculture, Kyoto University, Sakyoku, Kyoto 606-8502, Japan. tyamada@kais.kyoto-u.ac.jp
authors.forename: Takahisa
authors.initials: T
authors.lastname: Yamada
authors.forename: Seiki
authors.initials: S
authors.lastname: Sasaki
authors.forename: Shin
authors.initials: S
authors.lastname: Sukegawa
authors.forename: Youji
authors.initials: Y
authors.lastname: Muramatsu
authors.forename: Youichi
authors.initials: Y
authors.lastname: Takahagi
authors.forename: Sachiyo
authors.initials: S
authors.lastname: Yoshioka
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Fujita
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Morita
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Murakami
authors.forename: Fumiki
authors.initials: F
authors.lastname: Morimatsu
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Sasaki
mesh_terms: D000273:Adipose Tissue / Q000033:anatomy & histology; D000483:Alleles; D000818:Animals; D050218:Body Fat Distribution / Q000662:veterinary*; D001947:Breeding; D002417:Cattle / Q000033:anatomy & histology* / Q000235:genetics*; D008297:Male; D008840:Microfilament Proteins / Q000235:genetics*; D018482:Muscle, Skeletal / Q000033:anatomy & histology; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D030481:Pyruvate Dehydrogenase (Lipoamide) / Q000235:genetics*; D049349:Receptors, Lysosphingolipid / Q000235:genetics*
chemical_list: D008840:Microfilament Proteins; D049349:Receptors, Lysosphingolipid; C119201:ponsin; D030481:Pyruvate Dehydrogenase (Lipoamide)