title: Cloning and heterologous expression of Lactobacillus reuteri uroporphyrinogen III synthase/methyltransferase gene (cobA/hemD): preliminary characterization.
abstract: To clone, express and characterize uroporphyrinogen III synthase/methyltransferase gene (cobA/hemD) from Lactobacillus reuteri. Some strains of Lb. reuteri produce cobalamin (vitamin B(12)). Cobalamin biosynthesis relies on the sequential action of more than 25 enzymes in a complex metabolic pathway. We have cloned, expressed and characterized the gene in Lb. reuteri that codes for the S-adenosy L: -methionine uroprophyrinogen III methyltransferase/synthase (CobA/HemD), a key bifunctional enzyme in the biosynthesis of cobalamin and other tetrapyrrols.
authors.affiliation: Centro de Referencia para Lactobacilos, CERELA-CONICET, Chacabuco 145 (4000), San Miguel de Tucumán, Tucumán, Argentina.
authors.forename: Verónica
authors.initials: V
authors.lastname: Vannini
authors.forename: Adriana
authors.initials: A
authors.lastname: Rodríguez
authors.forename: José L
authors.initials: JL
authors.lastname: Vera
authors.forename: Graciela F
authors.initials: GF
authors.lastname: de Valdéz
authors.forename: María P
authors.initials: MP
authors.lastname: Taranto
authors.forename: Fernando
authors.initials: F
authors.lastname: Sesma
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D003198:Computer Simulation; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D052200:Limosilactobacillus reuteri / Q000201:enzymology* / Q000235:genetics; D008780:Methyltransferases / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D013050:Spectrometry, Fluorescence; D014576:Uroporphyrinogen III Synthetase / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D011994:Recombinant Proteins; D008780:Methyltransferases; C060508:uroporphyrin-III C-methyltransferase; D014576:Uroporphyrinogen III Synthetase