title: Uniformity trials in plant freezing assays involving microtitre plates.
abstract: The primary aim of this study was to measure the inherent experimental variability in plant freezing assays involving microtitre plates. Laurus nobilis leaf strips were used as experimental material. Data analysis involved variability measurements among and within microtitre plates. Statistically significant variability (p < 0.05) was observed in both cases. The second aim was to test the effectiveness of five experimental designs, in controlling the experimental error. According to our results the variability in microtitre plate freezing assays can be controlled by the use of blocked experimental designs and single well plots.
authors.affiliation: Department of Forestry and Management of Natural Environment, Technological Educational Institute of Larissa (Karditsa), Greece.
authors.forename: Dimitris
authors.initials: D
authors.lastname: Zaragotas
authors.forename: Elias
authors.initials: E
authors.lastname: Anastassopoulos
mesh_terms: D001681:Biological Assay / Q000592:standards*; D003080:Cold Temperature; D005069:Evaluation Studies as Topic; D005615:Freezing; D057166:High-Throughput Screening Assays / Q000592:standards*; D027442:Laurus; D018515:Plant Leaves; D010969:Plastics; D015203:Reproducibility of Results; D012107:Research Design
chemical_list: D010969:Plastics