title: Preparation of protein- and cell-resistant surfaces by hyperthermal hydrogen induced cross-linking of poly(ethylene oxide).
abstract: The functionalization of surfaces with poly(ethylene oxide) (PEO) is an effective means of imparting resistance to the adsorption of proteins and the attachment and growth of cells, properties that are critical for many biomedical applications. In this work, a new hyperthermal hydrogen induced cross-linking (HHIC) method was explored as a simple one-step approach for attaching PEO to surfaces through the selective cleavage of C-H bonds and subsequent cross-linking of the resulting carbon radicals. In order to study the effects of the process on the polymer, PEO-coated silicon wafers were prepared and the effects of different treatment times were investigated. Subsequently, using an optimized treatment time and a modified butyl polymer with increased affinity for PEO, the technique was applied to butyl rubber surfaces. All of the treated surfaces exhibited significantly reduced protein adsorption and cell growth relative to control surfaces and compared favorably with surfaces that were functionalized with PEO using conventional chemical methods. Thus HHIC is a simple and effective means of attaching PEO to non-functional polymer surfaces.
authors.affiliation: Department of Chemistry, The University of Western Ontario, 1151 Richmond Street, London, Canada N6A5B7.
authors.forename: Colin V
authors.initials: CV
authors.lastname: Bonduelle
authors.forename: Woon M
authors.initials: WM
authors.lastname: Lau
authors.forename: Elizabeth R
authors.initials: ER
authors.lastname: Gillies
mesh_terms: D000327:Adsorption; D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D005347:Fibroblasts / Q000502:physiology; D006859:Hydrogen / Q000737:chemistry*; D051379:Mice; D011092:Polyethylene Glycols / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D012825:Silicon / Q000737:chemistry; D013499:Surface Properties
chemical_list: D020099:Coated Materials, Biocompatible; D011506:Proteins; D011092:Polyethylene Glycols; D006859:Hydrogen; D012825:Silicon