title: A PNA microarray for tomato genotyping.
abstract: The design and development of a PNA microarray designed for the simultaneous identification of several SNPs characteristic of seven different tomato varieties is described. Highly selective arginine-based monomer containing PNAs (Arg-PNAs) have been used in order to obtain very selective probes. Seven modified PNA probes were synthesised and their binding properties in solution were studied. PNA-microarrays based on these probes were prepared and applied to SNP discrimination in model experiments using oligonucleotide mixtures simulating the different sequences of the seven tomato varieties. The strength and the limitations of such a system for SNP recognition are thoroughly discussed.
authors.affiliation: Dipartimento di Chimica Organica e Industriale, University of Parma, Parco Area delle Scienze 17a, I-43124, Parma, Italy.
authors.forename: Tullia
authors.initials: T
authors.lastname: Tedeschi
authors.forename: Alessandro
authors.initials: A
authors.lastname: Calabretta
authors.forename: Mariangela
authors.initials: M
authors.lastname: Bencivenni
authors.forename: Alex
authors.initials: A
authors.lastname: Manicardi
authors.forename: Giandomenico
authors.initials: G
authors.lastname: Corrado
authors.forename: Martina
authors.initials: M
authors.lastname: Caramante
authors.forename: Roberto
authors.initials: R
authors.lastname: Corradini
authors.forename: Rosa
authors.initials: R
authors.lastname: Rao
authors.forename: Stefano
authors.initials: S
authors.lastname: Sforza
authors.forename: Rosangela
authors.initials: R
authors.lastname: Marchelli
mesh_terms: D001483:Base Sequence; D005838:Genotype; D018551:Solanum lycopersicum / Q000235:genetics*; D015335:Molecular Probes / Q000138:chemical synthesis / Q000737:chemistry*; D015394:Molecular Structure; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis / Q000737:chemistry*; D020641:Polymorphism, Single Nucleotide; D044366:Transition Temperature
chemical_list: D015335:Molecular Probes; D020135:Peptide Nucleic Acids