title: High genetic diversity in field isolates of Trypanosoma theileri assessed by analysis of cathepsin L-like sequences disclosed multiple and new genotypes infecting cattle in Thailand.
abstract: In this study, we describe the first survey in Thailand of Trypanosoma theileri, a widespread and prevalent parasite of cattle that is transmitted by tabanid flies. Investigation of 210 bovine blood samples of Thai cattle from six farms by hematocrit centrifuge technique (HCT) revealed 14 samples with trypanosomes morphologically compatible to T. theileri. Additional animals were positive for T. theileri by PCR based on the Cathepsin L-like sequence (TthCATL-PCR) despite negative by HCT, indicating cryptic infections. Results revealed a prevalence of 26 ± 15% (95% CI) of T. theileri infection. Additionally, 12 samples positive for T. theileri were detected in cattle from other 11 farms. From a total of 30 blood samples positive by HCT and/or PCR from 17 farms, seven were characterized to evaluate the genetic polymorphism of T. theileri through sequence analysis of PCR-amplified CATL DNA sequences. All CATL sequences of T. theileri from Thai cattle clustered with sequences of the previously described phylogenetic lineages TthI and TthII, supporting only two major lineages of T. theileri in cattle around the world. However, 11 of the 29 CATL sequences analyzed showed to be different, disclosing an unexpectedly large polymorphic genetic repertoire, with multiple genotypes of T. theileri not previously described in other countries circulating in Thai cattle.
authors.affiliation: Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, Av. Prof. Lineu Prestes 1374, 05508-900 São Paulo, SP, Brazil.
authors.forename: Herakles A
authors.initials: HA
authors.lastname: Garcia
authors.forename: Ketsarin
authors.initials: K
authors.lastname: Kamyingkird
authors.forename: Adriana C
authors.initials: AC
authors.lastname: Rodrigues
authors.forename: Sathaporn
authors.initials: S
authors.lastname: Jittapalapong
authors.forename: Marta M G
authors.initials: MM
authors.lastname: Teixeira
authors.forename: Marc
authors.initials: M
authors.lastname: Desquesnes
mesh_terms: D000818:Animals; D001483:Base Sequence; D056668:Cathepsin L / Q000235:genetics / Q000378:metabolism*; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000469:parasitology*; D016054:DNA, Protozoan / Q000235:genetics; D014644:Genetic Variation; D005838:Genotype; D008969:Molecular Sequence Data; D058974:Phylogeography; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D013785:Thailand / Q000453:epidemiology; D014345:Trypanosoma / Q000235:genetics*; D014354:Trypanosomiasis, Bovine / Q000453:epidemiology / Q000469:parasitology*
chemical_list: D016054:DNA, Protozoan; D056668:Cathepsin L