title: Enhanced cardiac differentiation of mouse embryonic stem cells by use of the slow-turning, lateral vessel (STLV) bioreactor.
abstract: Embryoid body (EB) formation is a common intermediate during in vitro differentiation of pluripotent stem cells into specialized cell types. We have optimized the slow-turning, lateral vessel (STLV) for large scale and homogenous EB production from mouse embryonic stem cells. The effects of inoculating different cell numbers, time of EB adherence to gelatin-coated dishes, and rotation speed for optimal EB formation and cardiac differentiation were investigated. Using 3 × 10(5) cells/ml, 10 rpm rotary speed and plating of EBs onto gelatin-coated surfaces three days after culture, were the best parameters for optimal size and EB quality on consequent cardiac differentiation. These optimized parameters enrich cardiac differentiation in ES cells when using the STLV method.
authors.affiliation: BioTalentum Ltd., Aulich Lajos u. 26, Gödöllö, Hungary. nut_vs@yahoo.com
authors.forename: Sasitorn
authors.initials: S
authors.lastname: Rungarunlert
authors.forename: Nuttha
authors.initials: N
authors.lastname: Klincumhom
authors.forename: Istvan
authors.initials: I
authors.lastname: Bock
authors.forename: Csilla
authors.initials: C
authors.lastname: Nemes
authors.forename: Mongkol
authors.initials: M
authors.lastname: Techakumphu
authors.forename: Melinda K
authors.initials: MK
authors.lastname: Pirity
authors.forename: Andras
authors.initials: A
authors.lastname: Dinnyes
mesh_terms: D000818:Animals; D019149:Bioreactors; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology*; D048429:Cell Size; D058732:Embryoid Bodies / Q000166:cytology; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D020869:Gene Expression Profiling; D005819:Genetic Markers; D051379:Mice; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D005819:Genetic Markers