title: A new Penicillium echinulatum strain with faster cellulase secretion obtained using hydrogen peroxide mutagenesis and screening with 2-deoxyglucose.
abstract: OBJECTIVE
  The aim of this study is to improve cellulase production and secretion by Penicillium echinulatum using mutagenesis and selection in association with microfermentation and microanalysis methods.
  
  
  RESULTS
  A new genetic variant was isolated from strain 9A02S1 and named S1M29. It was obtained by mutagenesis with H₂O₂ and two screening steps, which involved selection in Petri dishes using the medium supplemented with 2-deoxyglucose and microfermentations in submerged culture. The mutant showed higher cellulase productivity than 9A02S1 based on the Filter Paper Activity assay and endoglucanase; the peak activities for these enzymes were reached significantly faster than for the parent strain.
  
  
  CONCLUSIONS
  The mutant obtained after mutagenesis and selection could produce and secrete cellulase faster than the parent strain.
  
  
  CONCLUSIONS
  Mutagenesis followed by selection is a useful tool for rapidly generating new cellulase-producing phenotypes in fungi. Faster production and higher titers of cellulases in mutant strains contribute to reduce the production costs for enzymatic complexes that hydrolyse lignocellulose residues and form fermented syrups, thus contributing to the economic production of bioethanol.
authors.affiliation: Enzyme and Biomass Laboratory, Institute of Biotechnology, University of Caxias do Sul, Caxias do Sul, RS, Brazil. ajpdillo@ucs.br
authors.forename: A J P
authors.initials: AJ
authors.lastname: Dillon
authors.forename: M
authors.initials: M
authors.lastname: Bettio
authors.forename: F G
authors.initials: FG
authors.lastname: Pozzan
authors.forename: T
authors.initials: T
authors.lastname: Andrighetti
authors.forename: M
authors.initials: M
authors.lastname: Camassola
mesh_terms: D002480:Cellulase / Q000235:genetics / Q000378:metabolism*; D003847:Deoxyglucose; D005285:Fermentation; D005658:Fungi / Q000235:genetics / Q000378:metabolism; D006861:Hydrogen Peroxide; D007218:Industrial Microbiology; D008031:Lignin / Q000378:metabolism; D016296:Mutagenesis; D010407:Penicillium / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification
chemical_list: C036909:lignocellulose; D008031:Lignin; D003847:Deoxyglucose; D006861:Hydrogen Peroxide; D002480:Cellulase