title: [Activation of calcium-sensing receptors is associated with apoptosis in cardiomyocytes under simulated ischemia/reperfusion].
abstract: To examine the association of activation of calcium-sensing receptors (CaSR) with apoptosis in cardiomyocytes under simulated ischemia/reperfusion.
  
  
  
  Ventricular cardiomyocytes of neonatal rats were incubated in ischemia-mimetic solution for 2 h, then re-incubated in normal culture medium for 24 h to establish a model of simulated ischemia/reperfusion (I/R). Cell apoptosis was detected by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL assay). The expression of CaSR mRNA was detected by reverse transcriptase polymerase chain reaction (RT-PCR). The expression of Caspase -3 and Bcl-2 was detected by Western blotting.
  
  
  
  The simulated I/R enhanced the expression of CaSR and cardiomyocyte apoptosis. GdCl(3), a specific activator of CaSR, further increased the expression of CaSR and cardiomyocyte apoptosis, along with upregulation of Caspase-3 and downregulation of Bcl-2.
  
  
  
  CaSR is associated with I/R injury and apoptosis in neonatal rat ventricular cardiomyocytes via suppressing Bcl-2 and promoting Caspase -3 expression.
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Yan
authors.forename: Tie-Bing
authors.initials: TB
authors.lastname: Zhu
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Sun
authors.forename: Shi-Yang
authors.initials: SY
authors.lastname: Pan
authors.forename: Lain-Sheng
authors.initials: LS
authors.lastname: Wang
authors.forename: Zheng-Xian
authors.initials: ZX
authors.lastname: Tao
authors.forename: Zhi-Jian
authors.initials: ZJ
authors.lastname: Yang
authors.forename: Ke-Jiang
authors.initials: KJ
authors.lastname: Cao
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D053148:Caspase 3 / Q000378:metabolism; D002478:Cells, Cultured; D015428:Myocardial Reperfusion Injury / Q000378:metabolism / Q000473:pathology*; D032383:Myocytes, Cardiac / Q000378:metabolism / Q000473:pathology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051381:Rats; D044169:Receptors, Calcium-Sensing / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2; D044169:Receptors, Calcium-Sensing; C505410:Casp3 protein, rat; D053148:Caspase 3