title: Chemiluminescence detection of label-free C-reactive protein based on catalytic activity of gold nanoparticles.
abstract: A novel, quantitative analytical method for measuring C-reactive protein (CRP) levels in human serum has been developed based on the catalytic activity of gold nanoparticles (GNPs) and luminol-H(2)O(2) chemiluminescence (CL). The CL intensity in the presence of CRP and its ligand, O-phosphorylethanolamine (PEA), was greatly enhanced due to the aggregation of GNPs after the addition of 0.5M NaCl. Any pretreatment steps, such as covalent functionalization of GNPs, addition of antibodies, or labeling of CRP, were not needed for CL detection. The CL enhancement was linearly proportional to CRP concentration in the range of 1.88 fM to 1.925 pM. The detection limit of CRP in serum samples was estimated to be as low as 1.88 fM. The detection sensitivity was increased more than 164 times of magnitude over that of the conventional, enzyme-linked immunosorbent assay (ELISA) method. This proposed GNP-based CL detection method offers the advantages of simplicity, rapidity, and sensitivity.
authors.affiliation: Department of Chemistry, Chonbuk National University, Jeonju 561-756, South Korea.
authors.forename: Md Shahinul
authors.initials: MS
authors.lastname: Islam
authors.forename: Seong Ho
authors.initials: SH
authors.lastname: Kang
mesh_terms: D002097:C-Reactive Protein / Q000032:analysis*; D002384:Catalysis; D006046:Gold / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D049449:Luminescence; D053768:Metal Nanoparticles; D046529:Microscopy, Electron, Transmission
chemical_list: D006046:Gold; D002097:C-Reactive Protein; D006861:Hydrogen Peroxide