title: Expression analyses of AtAGP17 and AtAGP19, two lysine-rich arabinogalactan proteins, in Arabidopsis.
abstract: AtAGP17 and AtAGP19 are members of the lysine-rich arabinogalactan protein (AGP) subfamily in Arabidopsis. Detailed anatomical analysis of promoter activity of the AtAGP19 gene was carried out using transgenic Arabidopsis plants expressing a P(AtAGP19):GUS fusion. AtAGP19 promoter activity was tissue-specific and associated with vascular bundles, particularly differentiating xylem elements. Peptide-specific antibodies were raised against the Lys-rich regions of AtAGP17 and AtAGP19 and used to study the organ-specific expression patterns of these two AGPs. AtAGP17 and AtAGP19 were most abundant in roots and flowers, moderately abundant in stems, seedlings and siliques and virtually absent in leaves. Antibodies specific for AtAGP17 and AtAGP19, as reported here, represent valuable tools for understanding the biology of these two AGPs.
authors.affiliation: College of Biological Science and Technology, Fuzhou University, Fuzhou, Fujian, China.
authors.forename: J
authors.initials: J
authors.lastname: Yang
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.forename: Y
authors.initials: Y
authors.lastname: Liang
authors.forename: A M
authors.initials: AM
authors.lastname: Showalter
mesh_terms: D000595:Amino Acid Sequence; D000906:Antibodies / Q000737:chemistry / Q000276:immunology; D000918:Antibody Specificity; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000096:biosynthesis* / Q000235:genetics* / Q000276:immunology; D015153:Blotting, Western; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008239:Lysine / Q000378:metabolism; D008969:Molecular Sequence Data; D009088:Mucoproteins / Q000096:biosynthesis* / Q000235:genetics* / Q000276:immunology; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic
chemical_list: D000906:Antibodies; D029681:Arabidopsis Proteins; D009088:Mucoproteins; D010940:Plant Proteins; C006619:arabinogalactan proteins; D008239:Lysine