title: Cells behaviour in presence of nano-scaffolds.
abstract: Nanomaterials are being made for use in various fields in biology from sensors to clutches. Nanomaterials may be perceived, in bizarre ways by the cells. Starting from the membrane to the nucleus, each component of the cell could have a new response. Mesenchymal Stem Cells (MSC) isolated from human placenta, Wharton's jelly, cord blood, fetal bone marrow and adipose tissue, cultured in the presence of biomaterials were monitored for differentiation in to the osteogenic phenotype. On the basis of osteogenic marker expression analysis, placental cells exhibited the best osteogenic potential among the different MSC. There is some cell death initially but the cells that remain viable show uptake of nanoparticles. The viable cells both divide and show differentiation into osteogenic cell type. The placental MSC in conjunction with the biomaterials were implanted in the injured femur of Wistar rats showed complete regeneration of the damaged region.
authors.affiliation: Centre for Cellular and Molecular Biology (Council of Scientific and Industrial Research, India), Hyderabad 500007, India.
authors.forename: G
authors.initials: G
authors.lastname: Sujatha
authors.forename: Arvind
authors.initials: A
authors.lastname: Sinha
authors.forename: Shashi
authors.initials: S
authors.lastname: Singh
mesh_terms: D018786:Bone Substitutes / Q000737:chemistry*; D002454:Cell Differentiation; D002478:Cells, Cultured; D004867:Equipment Design; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D050997:Nanomedicine / Q000295:instrumentation; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology; D010012:Osteogenesis / Q000502:physiology*; D054457:Tissue Scaffolds
chemical_list: D018786:Bone Substitutes