title: In vitro inhibitory effect of trichostatin A on canine grade 3 mast cell tumor.
abstract: Mast cell tumor (MCT) is one of the most prevalent neoplasms that affect skin and soft tissue in dogs. Because mast cell tumors present a great variety of clinical appearance and behavior, their treatment becomes a challenge. Trichostatin A (TSA), an antifungal antibiotic, has shown inhibitory effects on the proliferation and induction of apoptosis in various types of cancer cells. In order to evaluate the potential of trichostatin A as a therapeutic drug, cells of grade 3 MCT were cultured and treated with concentrations of 1 nM to 400 nM of TSA. MTT assay and trypan blue exclusion assays were performed to estimate cell growth and cell viability, and cell cycle analysis was evaluated. TSA treatment showed a reduction in numbers of viable cells and an increase of cell death by apoptosis. The cell cycle analysis showed an increase of hypodiploid cells and a reduction of G0/G1 and G2/M -phases. According to these results, trichostatin A may be an interesting potential chemotherapeutic agent for the treatment of canine MCT.
authors.affiliation: Laboratory of Experimental Oncology, Department of Pathology, School of Veterinary Medicine and Animal Science, University of São Paulo, SP, Brazil.
authors.forename: Marcia Kazumi
authors.initials: MK
authors.lastname: Nagamine
authors.forename: Daniel S
authors.initials: DS
authors.lastname: Sanches
authors.forename: Katia C
authors.initials: KC
authors.lastname: Pinello
authors.forename: Luciana Neves
authors.initials: LN
authors.lastname: Torres
authors.forename: Gregory
authors.initials: G
authors.lastname: Mennecier
authors.forename: Andréia O
authors.initials: AO
authors.lastname: Latorre
authors.forename: Heidge
authors.initials: H
authors.lastname: Fukumasu
authors.forename: Maria Lucia Zaidan
authors.initials: ML
authors.lastname: Dagli
mesh_terms: D000107:Acetylation; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004285:Dogs; D005260:Female; D056572:Histone Deacetylase Inhibitors / Q000008:administration & dosage / Q000494:pharmacology*; D006657:Histones / Q000378:metabolism; D006877:Hydroxamic Acids / Q000008:administration & dosage / Q000494:pharmacology*; D012515:Mast-Cell Sarcoma / Q000188:drug therapy / Q000473:pathology / Q000662:veterinary*; D051379:Mice; D008819:Mice, Nude; D013778:Tetrazolium Salts / Q000737:chemistry; D013844:Thiazoles / Q000737:chemistry; D014343:Trypan Blue / Q000737:chemistry
chemical_list: D056572:Histone Deacetylase Inhibitors; D006657:Histones; D006877:Hydroxamic Acids; D013778:Tetrazolium Salts; D013844:Thiazoles; C012589:trichostatin A; C022616:thiazolyl blue; D014343:Trypan Blue