title: Construction of a recombinant human FGF1 expression vector for mammary gland-specific expression in human breast cancer cells.
abstract: Human Fibroblast growth factor 1 (FGF1) has been recognized as a valuable protein drug for the treatment of many diseases because of its multiple functions in regulating a variety of biological processes involved in embryonic development, cell growth and differentiation, morphogenesis, tissue repair, and others. The aim of this study was to develop an FGF1 mammary gland-specific expression vector to produce FGF1 on a large scale from transgenic cows to meet the demand for FGF1 in medical use. In this study, we generated an FGF1 mammary gland-specific expression vector and validated its function in human MCF-7 cells. This vector was shown to successfully express functional FGF1, thus potentially enabling the generation of transgenic cows to be used as mammary gland bioreactors.
authors.affiliation: Department of Animal Biotechnology, College of Animal Science and Veterinary Medicine, Jilin University, Changchun, 130062 Jilin, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhou
authors.forename: Linzhu
authors.initials: L
authors.lastname: Ren
authors.forename: Jianguo
authors.initials: J
authors.lastname: Zhu
authors.forename: Sen
authors.initials: S
authors.lastname: Yan
authors.forename: Haijun
authors.initials: H
authors.lastname: Wang
authors.forename: Na
authors.initials: N
authors.lastname: Song
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Hongsheng
authors.initials: H
authors.lastname: Ouyang
authors.forename: Daxin
authors.initials: D
authors.lastname: Pang
mesh_terms: D000818:Animals; D001943:Breast Neoplasms; D002417:Cattle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003001:Cloning, Molecular / Q000379:methods*; D005260:Female; D005346:Fibroblast Growth Factors / Q000096:biosynthesis / Q000235:genetics*; D055786:Gene Knockout Techniques; D017930:Genes, Reporter; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D019426:Integrases / Q000235:genetics; D008321:Mammary Glands, Animal / Q000378:metabolism*; D009928:Organ Specificity; D010446:Peptide Fragments / Q000096:biosynthesis / Q000235:genetics*; D015202:Protein Engineering; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D010446:Peptide Fragments; D011994:Recombinant Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D005346:Fibroblast Growth Factors; C045073:Cre recombinase; D019426:Integrases