title: [Serum IL-18 levels in mice with collagen-induced arthritis treated by recombinant adenovirus containing mIL-18BP and mIL-4 fusion gene].
abstract: To investigate serum IL-18 levels in mice with collagen-induced arthritis treated by recombinant adenoviral vector containing mIL-18BP and mIL-4 fusion gene (AdmIL-18BP/mIL-4).
  
  
  
  Arthritis was induced by injection of collagen in male DBA-1/BOM mice. Mice with collagen-induced arthritis (CIA) were intra-articularly injected with 10(7)pfu/6μL of AdmIL-18BP/mIL-4; and in mice of control groups AdLacZ or PBS were used. The animals were sacrificed at week 1, 2 and 4 after treatment. Serum IL-18 levels were determined by ELISA at the different time points.
  
  
  
  The mean serum levels of IL-18 at weeks 1, 2, and 4 after injection of AdmIL-18BP/mIL-4 were (36.5±5.4)ng/L, (32.5 ± 3.2) ng/L and (28.7 ±2.9)ng/L, respectively, which were significantly lower than those at the same time point of AdLacZ group [(66.2 ±5.1)ng/L, (69.2 ±4.2)ng/L and (77.7 ±3.9)ng/L] and PBS group [(67.3 ±7.1)ng/L, (71.9 ±1.8)ng/L and (78.7±4.1)ng/L] (P<0.01 at all time points). In the therapy group, there were no significant differences in the mean serum concentrations of IL-18 at all time points.
  
  
  
  The serum IL-18 levels in CIA mice are down-regulated by treatment of recombinant adenovirus containing mIL-18BP and mIL-4 fuse gene, which might be a promising therapeutic strategy for rheumatoid arthritis.
authors.affiliation: Clinical Laboratory, the First Hangzhou People's Hospital, Hangzhou 310006, China. lengjh5@hotmail.com
authors.forename: Jian-Hang
authors.initials: JH
authors.lastname: Leng
authors.forename: Hang-Ping
authors.initials: HP
authors.lastname: Yao
authors.forename: Jun-Ya
authors.initials: JY
authors.lastname: Shen
authors.forename: Ke-Yi
authors.initials: KY
authors.lastname: Wang
authors.forename: Zi-Wei
authors.initials: ZW
authors.lastname: Wang
authors.forename: Guang-Chao
authors.initials: GC
authors.lastname: Zhuo
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D001169:Arthritis, Experimental / Q000097:blood / Q000628:therapy*; D050939:Gene Fusion; D015316:Genetic Therapy; D005822:Genetic Vectors; D020382:Interleukin-18 / Q000097:blood / Q000235:genetics*; D015847:Interleukin-4 / Q000235:genetics*; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA
chemical_list: D020382:Interleukin-18; D015847:Interleukin-4