title: Evaluation of pathogenic fungi occurrence in traumatogenic structures of freshwater fish.
abstract: BACKGROUND
  Fungal infections in human skin, such as sporotrichosis, can occur after fish induced trauma. This work aimed to identify fungi in freshwater fish that are pathogenic to humans.
  
  
  METHODS
  Extraction of dental arches from Serrassalmus maculatus (piranha) and Hoplias malabaricus (wolf fish), stings from Pimelodus maculatus (mandis catfish), dorsal fin rays from Plagioscion spp. (corvina) and Tilapia spp., for culture in Mycosel agar. Some cultures were submitted to DNA extraction for molecular identification by sequencing ITS-5.8S rDNA.
  
  
  RESULTS
  Cultures identified most yeast as Candida spp., while sequencing also permitted the identification of Phoma spp. and Yarrowia lipolytica.
  
  
  CONCLUSIONS
  While the search for S. schenckii was negative, the presence of fungus of the genera Phoma and Candida revealed the pathogenic potential of this infection route. The genus Phoma is involved in certain forms of phaeohyphomycosis, a subcutaneous mycosis caused by dematiaceous fungi, with reports of infections in human organs and systems. Traumatizing structures of some freshwater fish present pathogenic fungi and this may be an important infection route that must be considered in some regions of Brazil, since there are a large number of a fisherman in constant contact with traumatogenic fish.
authors.affiliation: Departamento de Dermatologia e Radioterapia, Faculdade de Medicina de Botucatu, Universidade Estadual Paulista, Botucatu, SP, Brasil.
authors.forename: Fabio Caetano Oliveira
authors.initials: FC
authors.lastname: Leme
authors.forename: Marcos Mendes de Barros
authors.initials: MM
authors.lastname: Negreiros
authors.forename: Fernando Akira
authors.initials: FA
authors.lastname: Koga
authors.forename: Sandra de Moraes Gimenes
authors.initials: Sde M
authors.lastname: Bosco
authors.forename: Eduardo
authors.initials: E
authors.lastname: Bagagli
authors.forename: Vidal
authors.initials: V
authors.lastname: Haddad Junior
mesh_terms: D000818:Animals; D001938:Brazil; D002175:Candida / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D004271:DNA, Fungal / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005398:Fisheries; D005399:Fishes / Q000145:classification / Q000382:microbiology*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D045483:Rivers; D025062:Yarrowia / Q000235:genetics* / Q000472:pathogenicity
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal