title: Serum resistance to singlet oxygen in patients with diabetes mellitus in comparison to healthy donors.
abstract: Diabetes mellitus causes endothelial injury through oxidative stress involving reactive oxygen species and peroxides as well as inflammation, both of which consume antioxidant defenses. Singlet oxygen ((1)O(2)) is produced by leukocytes during inflammatory and biochemical reactions and deactivated by producing reactive oxygen species and peroxides. To determine whether serum was capable of deactivating (1)O(2), we triggered a photo reaction in sera from 53 healthy donors and 52 diabetic patients. Immediately after light delivery, dichlorofluorescein was added and then its fluorescence was recorded. The mean capacity of (1)O(2) or secondary oxidant deactivation was reduced in patients with diabetes mellitus. Hemolysis reduced deactivation of (1)O(2)-induced secondary oxidants in both healthy and diabetic patients. Body mass index, age, platelet counts, and blood cell numbers exerted a nonlinear influence. High levels of glycated hemoglobin were associated with an increased deactivation of oxidative species, whereas high-density lipoprotein cholesterol, total cholesterol, and the total cholesterol to high-density lipoprotein cholesterol ratio decreased the serum deactivation capacity. Oral antidiabetics bore no influence on deactivation, which was restored by insulin in women. Deactivation capacity was lower in women, who had half the complications found in men, suggesting that, with more severe diabetes mellitus, protection was maintained against complications. Resistance to (1)O(2) should be considered during the monitoring of diabetes mellitus.
authors.affiliation: Laser Department, Laënnec Hospital, 44093 Nantes, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Lhommeau
authors.forename: Samuel
authors.initials: S
authors.lastname: Douillard
authors.forename: Edith
authors.initials: E
authors.lastname: Bigot
authors.forename: Isabelle
authors.initials: I
authors.lastname: Benoit
authors.forename: Michel
authors.initials: M
authors.lastname: Krempf
authors.forename: Thierry
authors.initials: T
authors.lastname: Patrice
mesh_terms: D000328:Adult; D019540:Area Under Curve; D003920:Diabetes Mellitus / Q000378:metabolism*; D005260:Female; D006442:Glycated Hemoglobin / Q000032:analysis; D006801:Humans; D008297:Male; D008875:Middle Aged; D017382:Reactive Oxygen Species / Q000378:metabolism; D026082:Singlet Oxygen / Q000097:blood / Q000378:metabolism*
chemical_list: D006442:Glycated Hemoglobin; D017382:Reactive Oxygen Species; D026082:Singlet Oxygen; C517652:hemoglobin A1c protein, human