title: Cryopreservation of goldfish caudal fin explants using glycerol as a cryoprotecant.
abstract: The objective of this study was to investigate the effect of glycerol on the cryopreservation fin explants of goldfish, Carassius auratus. Four different concentrations, 5, 10, 15, and 20% (v/v) of glycerol and a control were tested. These were prepared in Dulbecco's modified Eagle's medium with 20% (v/v) Fetal Bovine Serum. Attachment and outgrowing rates were monitored from day 3 to day 14. Results showed that fin explants cryopreserved in 20% concentration of glycerol was significantly higher (P < 0.05) with a 100% attachment rate compared to 5, 10, and 15% concentrations with 36.67, 84.19 and 86.51% attachment rate, respectively. Fin explants cryopreserved in 20% glycerol concentration also had significantly higher (P < 0.05) outgrowth of cells (73%) than the other three concentrations on day 3. Moreover, a 100% outgrowth of cells in all concentrations was achieved after 14 days of culture. No attachment and out growth of cells were observed in control group. Goldfish caudal fin explants cryopreserved in glycerol can produce live cells efficiently, regardless of concentration.
authors.affiliation: Laboratory of Aquatic Animal Medicine, College of Veterinary Medicine, Seoul National University, Seoul, South Korea.
authors.forename: Casiano H
authors.initials: CH
authors.lastname: Choresca
authors.forename: Denis K
authors.initials: DK
authors.lastname: Gomez
authors.forename: Ji Hyung
authors.initials: JH
authors.lastname: Kim
authors.forename: Jee Eun
authors.initials: JE
authors.lastname: Han
authors.forename: Sang Phil
authors.initials: SP
authors.lastname: Shin
authors.forename: Byeong Chun
authors.initials: BC
authors.lastname: Lee
authors.forename: Se Chang
authors.initials: SC
authors.lastname: Park
mesh_terms: D058500:Animal Fins / Q000166:cytology* / Q000187:drug effects*; D000818:Animals; D002448:Cell Adhesion; D002452:Cell Count; D018929:Cell Culture Techniques; D003080:Cold Temperature; D015925:Cryopreservation; D003451:Cryoprotective Agents / Q000494:pharmacology*; D005990:Glycerol / Q000494:pharmacology*; D006054:Goldfish
chemical_list: D003451:Cryoprotective Agents; D005990:Glycerol