title: Effect of coupled plasma filtration adsorption on endothelial cell function in patients with multiple organ dysfunction syndrome.
abstract: OBJECTIVE
  The purpose of our study was to investigate the effect of coupled plasma filtration adsorption (CPFA) on endothelial cell (EC) function in patients with multiple organ dysfunction syndrome (MODS).
  
  
  METHODS
  Besides routine therapy, the 24 MODS patients underwent both CPFA and high volume hemofiltration (HVHF), scheduled randomly at intervals of 12 hours. Patient serum from 0, 5, and 10 hours of therapy was collected to measure soluble E-selectin (sE-selectin) and soluble thrombomodulin (sTM) by the ELISA method. Human umbilical vein endothelial cells (HUVEC) were incubated for 24 hours with the patient serum and the supernatant liquid was gathered to detect sTM and sE-selectin. The proliferation function of the ECs was detected by methyl thiazolyl tetrazolium (MTT) method.
  
  
  RESULTS
  1. The serum levels of sE-selectin and sTM were significantly higher in MODS patients than in controls; serum sE-selectin and sTM decreased remarkably after a single circulation in CPFA (p<0. 05) but not in HVHF (p>0. 05); the level of sE-selectin and sTM in systemic circulation had no change during CPFA or HVHF (p>0.05); 2. sTM in supernatant liquid incubated with serum from 5 hours of CPFA and 10 hours of HVHF decreased remarkably (p<0.05), while sE-selectin decreased significantly (p<0. 05) from 10 hours of CPFA , but there was no change from 5 hours and 10 hours of HVHF (p>0. 05); 3. when incubated with serum taken from the device pre- or post-CPFA, the optical density (OD) value of the latter was higher. The OD value increased gradually when incubated with serum from 0, 5, and 10 hours of CPFA (p<0.05), but changed little from HVHF.
  
  
  CONCLUSIONS
  CPFA can eliminate sE-selectin and sTM and improve the secretion function of ECs. CPFA was somewhat better and earlier than HVHF, while to a certain degree it can weaken the inhibitory effect of serum on the proliferation function of ECs.
authors.affiliation: Department of Nephrology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu Province, China.
authors.forename: Huijuan
authors.initials: H
authors.lastname: Mao
authors.forename: Shu
authors.initials: S
authors.lastname: Yu
authors.forename: Xiangbao
authors.initials: X
authors.lastname: Yu
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Jianming
authors.initials: J
authors.lastname: Hu
authors.forename: Xianrong
authors.initials: X
authors.lastname: Xu
authors.forename: Yamei
authors.initials: Y
authors.lastname: Zhu
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Wang
authors.forename: Changying
authors.initials: C
authors.lastname: Xing
mesh_terms: D000328:Adult; D000368:Aged; D049109:Cell Proliferation; D019040:E-Selectin / Q000097:blood; D042783:Endothelial Cells / Q000502:physiology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006440:Hemofiltration / Q000379:methods*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009102:Multiple Organ Failure / Q000097:blood* / Q000503:physiopathology / Q000628:therapy*; D018180:Thrombomodulin / Q000097:blood
chemical_list: D019040:E-Selectin; D018180:Thrombomodulin