title: Production of succinate and polyhydroxyalkanoate from substrate mixture by metabolically engineered Escherichia coli.
abstract: The strategic design of this study aimed at producing succinate and polyhydroxyalkanoate (PHA) from substrate mixture of glycerol/glucose and fatty acid in Escherichia coli. To accomplish this, an E. coli KNSP1 strain derived from E. coli LR1110 was constructed by deletions of ptsG, sdhA and pta genes and overexpression of phaC1 from Pseudomonas aeruginosa. Cultivation of E. coli KNSP1 showed that this strain was able to produce 21.07 g/L succinate and 0.54 g/L PHA (5.62 wt.% of cell dry weight) from glycerol and fatty acid mixture. The generated PHA composed of 58.7 mol% 3-hydroxyoctanoate (3HO) and 41.3 mol% 3-hydroxydecanoate (3HD). This strain would be useful for complete utilization of byproducts glycerol and fatty acid of biodiesel production process.
authors.affiliation: State Key Laboratory of Microbial Technology, Life Science School, Shandong University, Jinan 250100, PR China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Kang
authors.forename: Lili
authors.initials: L
authors.lastname: Du
authors.forename: Junhua
authors.initials: J
authors.lastname: Kang
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.forename: Quanfeng
authors.initials: Q
authors.lastname: Liang
authors.forename: Qingsheng
authors.initials: Q
authors.lastname: Qi
mesh_terms: D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D005818:Genetic Engineering / Q000379:methods*; D005990:Glycerol / Q000378:metabolism; D054813:Polyhydroxyalkanoates / Q000378:metabolism*; D013379:Substrate Specificity; D019802:Succinic Acid / Q000138:chemical synthesis*
chemical_list: D054813:Polyhydroxyalkanoates; D019802:Succinic Acid; D005990:Glycerol