title: Effects of verbascoside, biotechnologically purified by Syringa vulgaris plant cell cultures, in a rodent model of periodontitis.
abstract: OBJECTIVE
  Verbascoside has previously been characterized as an effective scavenger of active free radicals and an inhibitor of lipid peroxidation. In the present study, we have investigated the effects of verbascoside from Syringa vulgaris in a rat model of ligature-induced periodontitis.
  
  
  METHODS
  Male Sprague-Dawley rats were lightly anaesthetized with pentobarbitone (35 mg/kg). Sterile, 2-0 black braided silk thread was placed around the cervix of the lower left first molar and knotted medially. Animals received vebascoside 2 mg/kg orally, daily for 8 days.
  
  
  RESULTS
  On the eighth day after placement of the ligature, we evaluated several markers of inflammation: (i) myeloperoxidase activity, (ii) thiobarbituric acid-reactant substance measurements, (iii) NF-κB expression, (iv) iNOS expression, (v) the nitration of tyrosine residues, (vi) activation of the nuclear enzyme poly(ADP-ribose) polymerase, (vii) Bax and Bcl-2 expression and (viii) a degree of gingivomucosal tissue injury. Oral administration of verbascoside (2 mg/kg daily for 8 days) significantly decreased all of the parameters of inflammation as described above.
  
  
  CONCLUSIONS
  These results demonstrate that verbascoside exerts an anti-inflammatory role during experimental periodontitis and is able to ameliorate the tissue damage associated with ligature-induced periodontitis.
authors.affiliation: IRCCS Centro Neurolesi Bonino-Pulejo, Messina, Italy.
authors.forename: Rosanna D I
authors.initials: RD
authors.lastname: Paola
authors.forename: Giacomo
authors.initials: G
authors.lastname: Oteri
authors.forename: Emanuela
authors.initials: E
authors.lastname: Mazzon
authors.forename: Concetta
authors.initials: C
authors.lastname: Crisafulli
authors.forename: Maria
authors.initials: M
authors.lastname: Galuppo
authors.forename: Roberto D A L
authors.initials: RD
authors.lastname: Toso
authors.forename: Giovanna
authors.initials: G
authors.lastname: Pressi
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Cordasco
authors.forename: Salvatore
authors.initials: S
authors.lastname: Cuzzocrea
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D015415:Biomarkers / Q000378:metabolism; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005881:Gingiva / Q000187:drug effects / Q000473:pathology; D005960:Glucosides / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D018836:Inflammation Mediators / Q000378:metabolism*; D008026:Ligation; D008297:Male; D008963:Molar; D009061:Mouth Mucosa / Q000187:drug effects / Q000473:pathology; D010518:Periodontitis / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D031662:Syringa / Q000737:chemistry*; D059808:Polyphenols
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D015415:Biomarkers; D005960:Glucosides; D018836:Inflammation Mediators; D010636:Phenols; D010936:Plant Extracts; C058956:acteoside; D059808:Polyphenols