title: Boron-containing two-photon-absorbing chromophores. 3. One- and two-photon photophysical properties of p-carborane-containing fluorescent bioprobes.
abstract: Boron-containing two-photon-absorbing fluorophores have been prepared as new bifunctional molecules, potentially useful in two-photon excited microscopy (TPEM) and boron neutron capture therapy. They are based on a one-dimensional conjugated system containing a p-carborane entity at one end of the molecule and various electron-donating groups containing oxygen or nitrogen atoms at the other end. We investigated their one- and two-photon photophysical properties. They showed efficient fluorescence in an organic solvent, as well as in water for two of them, allowing microscopy on cell cultures. High two-photon absorption cross sections were determined in the 700-900 nm range. TPEM images were obtained with these new p-carborane-containing fluorophores, with laser intensities in the submilliwatt range.
authors.affiliation: Laboratoire de Biophotonique et Pharmacologie, Université de Strasbourg, CNRS (UMR 7213), Faculté de Pharmacie, 74 route du Rhin, F-67401 Illkirch Graffenstaden Cedex, France. jean-francois.nicoud@unistra.fr
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Nicoud
authors.forename: Frédéric
authors.initials: F
authors.lastname: Bolze
authors.forename: Xiao-Hua
authors.initials: XH
authors.lastname: Sun
authors.forename: Ali
authors.initials: A
authors.lastname: Hayek
authors.forename: Patrice
authors.initials: P
authors.lastname: Baldeck
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D001880:Boranes / Q000138:chemical synthesis* / Q000378:metabolism; D016754:Boron Neutron Capture Therapy; D005260:Female; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D007834:Lasers; D008027:Light; D036641:Microscopy, Fluorescence, Multiphoton; D055668:Photochemical Processes / Q000528:radiation effects; D017785:Photons
chemical_list: D001880:Boranes; D005456:Fluorescent Dyes