title: Immune response and pathophysiological features of Klebsiella pneumoniae liver abscesses in an animal model.
abstract: Capsular serotypes K1 and K2, the rmpA gene (a regulator of the mucoid phenotype) and aerobactin from Klebsiella pneumoniae have been identified as the major virulence factors for pyogenic liver abscesses with high morbidity, mortality and severe complications. The pathological mechanisms remain unclear. In this study, we compared liver immune responses and pathological changes in response to different serotypes of K. pneumoniae infections. A mouse model was used to investigate cytokine and chemokine production, histopathology findings, phagocytic uptake and mortality induced by serotypes K1 (magA(+), rmpA(+), aerobactin(+)), K2 (magA(-), rmpA(+), aerobactin(+)), K62 (magA(-), rmpA(-), aerobactin(-)) and an acapsulated isogenic K1 mutant (ΔK1, magA(+), rmpA(+), aerobactin(+)). K. pneumoniae serotypes K1 and K2 showed lower 50% lethal dose values and more phagocytic resistance to neutrophils than K62 and the ΔK1 mutant. In sequential liver samples, viable bacteria counts increased 3 h to 3 days after low-dose inoculation (<10(1) colony-forming unit (cfu)) with K1 and K2, while K62 and ΔK1 cleared rapidly and became undetectable even with high-dose inoculation (∼2.9 × 10(5) cfu). Time-dependent increases in cytokines and chemokines, including tumor necrosis factor-α, interleukin (IL)-1β, IL-6, IL-10, keratinocyte-derived chemokines and macrophage inflammatory protein-2, were observed in the serum and liver tissue of K1- and K2-infected mice, and severe disease progression manifesting as microabscesses was also identified. K62 and ΔK1 inoculation did not result in similar immune responses and histological changes. These findings illustrate the critical role of phagocytic resistance against innate immunological defense mechanisms as well as its contribution to the development of liver abscesses.
authors.affiliation: Division of Infectious Diseases, Department of Medicine, Taipei Veterans General Hospital and National Yang-Ming University, Taiwan, ROC. cpfung@vghtpe.gov.tw
authors.forename: Chang-Phone
authors.initials: CP
authors.lastname: Fung
authors.forename: Feng-Yee
authors.initials: FY
authors.lastname: Chang
authors.forename: Jung-Chung
authors.initials: JC
authors.lastname: Lin
authors.forename: Donald Ming-Tak
authors.initials: DM
authors.lastname: Ho
authors.forename: Chiung-Tong
authors.initials: CT
authors.lastname: Chen
authors.forename: Jiun-Han
authors.initials: JH
authors.lastname: Chen
authors.forename: Kuo-Ming
authors.initials: KM
authors.lastname: Yeh
authors.forename: Te-Li
authors.initials: TL
authors.lastname: Chen
authors.forename: Yi-Tsung
authors.initials: YT
authors.lastname: Lin
authors.forename: L Kristopher
authors.initials: LK
authors.lastname: Siu
mesh_terms: D000818:Animals; D001483:Base Sequence; D015169:Colony Count, Microbial; D017931:DNA Primers; D004195:Disease Models, Animal; D007711:Klebsiella pneumoniae / Q000302:isolation & purification*; D008100:Liver Abscess / Q000276:immunology* / Q000382:microbiology / Q000503:physiopathology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010587:Phagocytosis
chemical_list: D017931:DNA Primers