title: [Comparison and significance of DCDF probe and ES probe in the detection of BCR/ABL fusion gene].
abstract: OBJECTIVE
  To investigate the signal patterns of dual color dual fusion (DCDF) probe and extra signal (ES) probe in the detection of BCR/ABL fusion gene, and illustrate the relation between the fluorescence in situ hybridization (FISH) pattern and the karyotype.
  
  
  METHODS
  Sixty-five cases of chronic myelocytic leukemia (CML) and 50 cases of acute lymphoblastic leukemia (ALL) were detected by FISH with DCDF probe, the BCR/ABL positive samples were detected by FISH with ES probe. Among these cases, 47 cases of CML and 40 cases of ALL perform conventional cytogenetics simultaneously.
  
  
  RESULTS
  All 65 cases of CML were all BCR/ABL positive by FISH. 17 cases showed the atypical pattern by DCDF-FISH, and 12 cases showed the atypical pattern by ES-FISH. There were 7 cases of BCR/ABL positive in 50 cases of ALL by FISH. By ES-FISH, there were 5 cases in which the break-point of BCR gene was located in m-bcr, 2 cases in which the break-point of BCR gene was located in M-bcr. Conventional cytogenetics demonstrated that 43/44(98%) cases of CML and 7/32(22%) cases of ALL were Ph positive.
  
  
  CONCLUSIONS
  The features of DCDF-FISH, ES-FISH and conventional cytogenetic are different from each other. According to the features of these method, it can increase the precision of the adjustment of genetic feature to analyze these results comprehensively.
authors.affiliation: Department of Hematology, Guangzhou First Municipal People's Hospital, Guangzhou, Guangdong, 510180 P. R. China. du_haven@yahoo.com.cn
authors.forename: Qing-hua
authors.initials: QH
authors.lastname: DU
authors.forename: Yi
authors.initials: Y
authors.lastname: YING
authors.forename: Xiao-yan
authors.initials: XY
authors.lastname: CHEN
authors.forename: Qing-shan
authors.initials: QS
authors.lastname: LI
authors.forename: Shun-qing
authors.initials: SQ
authors.lastname: WANG
authors.forename: Wen-jian
authors.initials: WJ
authors.lastname: MO
mesh_terms: D000328:Adult; D003582:Cytogenetics / Q000379:methods; D015342:DNA Probes; D005260:Female; D016044:Fusion Proteins, bcr-abl / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007621:Karyotyping / Q000379:methods; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics*; D008297:Male; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics
chemical_list: D015342:DNA Probes; D016044:Fusion Proteins, bcr-abl