title: Incorporation of endothelial progenitor cells into mosaic pseudoislets.
abstract: Pancreatic islet transplantation is limited by extensive apoptosis and suboptimal function of the implanted islets in the longer term. Endothelial progenitor cells (EPC) may be ideal for enhancing both the survival and function of transplanted islets. Here, we describe for the first time the in vitro formation of rat mosaic pseudoislets comprised of pancreatic β-cells with interspersed vasculogenic EPC. Bone marrow-derived EPC displayed a similar phenotype to non-adherent EPC, recently described in the human and mouse. Mosaic pseudoislet formation was enhanced by the use of an embryoid body forming medium (BPEL) and a spin protocol. Mosaic pseudoislets maintained function in vitro and may represent an enhanced cell therapy delivery approach to enhance the survival and revascularisation of transplanted islets.
authors.affiliation: Islet Transplantation and Dendritic Cell Biology Laboratory, Central Northern Adelaide Renal and Transplantation Service, Royal Adelaide Hospital, Adelaide, Australia.
authors.forename: Daniella
authors.initials: D
authors.lastname: Penko
authors.forename: Daisy
authors.initials: D
authors.lastname: Mohanasundaram
authors.forename: Shaundeep
authors.initials: S
authors.lastname: Sen
authors.forename: Christopher
authors.initials: C
authors.lastname: Drogemuller
authors.forename: Claire
authors.initials: C
authors.lastname: Mee
authors.forename: Claudine S
authors.initials: CS
authors.lastname: Bonder
authors.forename: P Toby H
authors.initials: PT
authors.lastname: Coates
authors.forename: Claire F
authors.initials: CF
authors.lastname: Jessup
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D044842:Cullin Proteins / Q000502:physiology; D042783:Endothelial Cells / Q000166:cytology*; D005434:Flow Cytometry; D007515:Islets of Langerhans / Q000166:cytology*; D016381:Islets of Langerhans Transplantation / Q000379:methods; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D018613:Microscopy, Confocal; D051381:Rats; D017208:Rats, Wistar; D017483:Receptors, Vasopressin / Q000502:physiology; D013234:Stem Cells / Q000166:cytology*; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000502:physiology
chemical_list: C094547:Cul5 protein, rat; D044842:Cullin Proteins; D017483:Receptors, Vasopressin; D040301:Vascular Endothelial Growth Factor Receptor-2