title: A novel composition for in vitro and in vivo regeneration of skin and connective tissues.
abstract: The particular combination of polydeoxyribonucleotides, l-carnitine, calcium ions, proteolytic enzyme and other ingredients acts in a synergetic way in the regeneration of skin and connective tissues. This new formulation of active principles was tested in vitro as a cell and tissue culture medium and in vivo for various preparations in support of tissue regeneration. In vitro, the new blend allowed the maintenance of skin biopsies for more than 1 year in eutrophic conditions. Immunocytochemical analyses of fibroblasts isolated from these biopsies confirmed a significant increase of the epidermal and connective wound-healing markers such as collagen type I, collagen type IV, cytokeratin 1 (CK1), CK5, CK10 and CK14 versus controls. To examine the effects of the new compound in vivo, we studied impaired wound healing in genetically diabetic db/db mice. At day 18, diabetic mice treated with the new composition showed 100% closure of wounds and faster healing than mice treated with the other solutions. This complex of vital continuity factors or life-keeping factors could be used as a tissue-preserving solution or a cosmetic/drug/medical device to accelerate wound healing in the treatment of patients with deficient wound repair to promote the regeneration of cutaneous and connective tissues (injuries-wound, dermatitis) and prevent the recurrent relapses.
authors.affiliation: Department of Genetics, Biology and Biochemistry, University of Turin, Turin, Italy. luisagennero@yahoo.com
authors.forename: Luisa
authors.initials: L
authors.lastname: Gennero
authors.forename: Rocco
authors.initials: R
authors.lastname: De Siena
authors.forename: Tetyana
authors.initials: T
authors.lastname: Denysenko
authors.forename: Maria Augusta
authors.initials: MA
authors.lastname: Roos
authors.forename: Gian Franco
authors.initials: GF
authors.lastname: Calisti
authors.forename: Manuela
authors.initials: M
authors.lastname: Martano
authors.forename: Simona
authors.initials: S
authors.lastname: Fiobellot
authors.forename: Michele
authors.initials: M
authors.lastname: Panzone
authors.forename: Stefano
authors.initials: S
authors.lastname: Reguzzi
authors.forename: Luisa
authors.initials: L
authors.lastname: Gabetti
authors.forename: Andrea
authors.initials: A
authors.lastname: Vercelli
authors.forename: Giovanni
authors.initials: G
authors.lastname: Cavallo
authors.forename: Elia
authors.initials: E
authors.lastname: Ricci
authors.forename: Gian Piero
authors.initials: GP
authors.lastname: Pescarmona
mesh_terms: D000287:Administration, Topical; D000818:Animals; D001706:Biopsy; D001835:Body Weight; D002470:Cell Survival; D002478:Cells, Cultured; D002626:Chemistry, Pharmaceutical; D024042:Collagen Type I / Q000378:metabolism; D024043:Collagen Type II / Q000378:metabolism; D003238:Connective Tissue / Q000187:drug effects / Q000254:growth & development*; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology*; D003920:Diabetes Mellitus / Q000473:pathology; D005347:Fibroblasts / Q000187:drug effects / Q000502:physiology; D006801:Humans; D007150:Immunohistochemistry; D007633:Keratins / Q000378:metabolism; D008297:Male; D051379:Mice; D011089:Polydeoxyribonucleotides / Q000494:pharmacology; D012867:Skin / Q000187:drug effects / Q000254:growth & development*; D013194:Staining and Labeling / Q000379:methods; D014945:Wound Healing
chemical_list: D024042:Collagen Type I; D024043:Collagen Type II; D003470:Culture Media; D011089:Polydeoxyribonucleotides; D007633:Keratins