title: Proteomic analysis of protective effects of polysaccharides from Salvia miltiorrhiza against immunological liver injury in mice.
abstract: This study was designed to investigate mechanisms of the protective effects of Salvia miltiorrhiza polysaccharide (SMPS) against lipopolysaccharide (LPS)-induced immunological liver injury (ILI) in Bacille Calmette-Guérin (BCG)-primed mice. Two-dimensional difference gel electrophoresis (2D-DIGE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis showed that three proteins are down-regulated and six proteins are up-regulated by SMPS. SMPS reduces the degree of liver injury by up-regulating the enzymes of the citric acid cycle, namely malate dehydrogenase (MDH) and 2-oxoglutarate dehydrogenase complex. LPS significantly increases nuclear factor kappa B (NF-κB) activation, inducible nitric oxide synthase (iNOS) expression and MDA level in BCG primed mice liver, whereas SMPS treatment protects against the immunological liver injury through inhibition of the NF-κB activation by up-regulation of PRDX6 and the subsequent attenuation of lipid peroxidation, iNOS expression and inflammation.
authors.affiliation: The Key Laboratory of Molecular Biology, State Administration of Traditional Chinese Medicine, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong Province, China.
authors.forename: Xue-Gang
authors.initials: XG
authors.lastname: Sun
authors.forename: Xiu-Qiong
authors.initials: XQ
authors.lastname: Fu
authors.forename: Hong-Bing
authors.initials: HB
authors.lastname: Cai
authors.forename: Qiang
authors.initials: Q
authors.lastname: Liu
authors.forename: Chun-Hua
authors.initials: CH
authors.lastname: Li
authors.forename: Ya-Wei
authors.initials: YW
authors.lastname: Liu
authors.forename: Ying-Jia
authors.initials: YJ
authors.lastname: Li
authors.forename: Zhi-Feng
authors.initials: ZF
authors.lastname: Liu
authors.forename: Yu-Hong
authors.initials: YH
authors.lastname: Song
authors.forename: Zhi-Ping
authors.initials: ZP
authors.lastname: Lv
mesh_terms: D000818:Animals; D008070:Lipopolysaccharides; D008099:Liver / Q000201:enzymology / Q000276:immunology / Q000473:pathology*; D008315:Malondialdehyde / Q000032:analysis; D051379:Mice; D008815:Mice, Inbred Strains; D016328:NF-kappa B / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D054465:Peroxiredoxin VI / Q000378:metabolism; D011134:Polysaccharides / Q000494:pharmacology*; D040901:Proteomics; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D057825:Two-Dimensional Difference Gel Electrophoresis
chemical_list: D008070:Lipopolysaccharides; D016328:NF-kappa B; D011134:Polysaccharides; D008315:Malondialdehyde; D054465:Peroxiredoxin VI; C515983:Prdx6 protein, mouse; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse