title: Tenocyte apoptosis in the torn rotator cuff: a primary or secondary pathological event?
abstract: Little information exists on the contribution of apoptosis to pathological tendon changes in rotator cuff tendinopathy. The purpose of this study was to quantitate the rate of tenocyte apoptosis in torn supraspinatus tendons and in the matched intact subscapularis and to examine the potential relation between apoptotic index (AI) and tendon pathology. In addition, the authors examined tenocyte density, proliferation rate and p53 gene expression patterns to gain further insight into relevant pathological mechanisms in the torn suprapinatus. 15 torn supraspinatus tendons with matched intact subscapularis tendon samples and 10 reference subscapularis samples were collected. Immunohistochemistry was used to define the AI (F7-26), proliferation rate (Ki67) and presence of p53 (M7001). Tendon degeneration was evaluated according to the Bonar scale. Expression of p53 and relevant genes (n=84) was examined on a subset of samples using microfluidic arrays. The AI was significantly increased in torn supraspinatus tendon and matched subscapularis tendon (R² =0.5742; p=0.0005). Cell density and proliferation rate were also elevated in torn supraspinatus compared with reference subscapularis tendons (p<0.05). A significant increase in p53 occurred specifically in torn supraspinatus tendon (p<0.05), and several genes encoding p53-inhibiting proteins were downregulated in association, including HDAC1 (p<0.05), MDM4 (p<0.001) and PPM1D (p<0.05). Our results suggest that tenocyte apoptosis results from more than one mechanism in the injured rotator cuff, including both intrinsic factors related specifically to the torn supraspinatus tendon, as well as a more generalised effect on the adjacent subscapularis tendon.
authors.affiliation: Department of Orthopaedic.Surgery, Lovisenberg Deaconal.Hospital, Oslo, Norway. kirsten.lundgreen@lds.no
authors.forename: Kirsten
authors.initials: K
authors.lastname: Lundgreen
authors.forename: Oystein Bjerkestrand
authors.initials: OB
authors.lastname: Lian
authors.forename: Lars
authors.initials: L
authors.lastname: Engebretsen
authors.forename: Alex
authors.initials: A
authors.lastname: Scott
mesh_terms: D000368:Aged; D017209:Apoptosis / Q000235:genetics / Q000502:physiology*; D049109:Cell Proliferation; D005260:Female; D015870:Gene Expression; D016158:Genes, p53; D006801:Humans; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000378:metabolism; D017006:Rotator Cuff / Q000473:pathology; D000070636:Rotator Cuff Injuries; D012421:Rupture / Q000473:pathology; D052256:Tendinopathy / Q000473:pathology*; D016159:Tumor Suppressor Protein p53 / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D016159:Tumor Suppressor Protein p53