title: Isolation and characterization of proline/betaine transporter gene from oil palm.
abstract: Oil production from oil palm is adversely affected by drought and salt. Under drought and salt stress, proline content increases in oil palm; the mechanism for this is unknown. Here, an 8319-nucleotide sequence including cDNA, genomic DNA and the promoter region of proline transporter gene from oil palm Elaeis guineensis was determined. The transporter gene exhibited high similarity to Bet/ProT genes from several plants, but the highest homology was found with rice ProT1. The exon-intron structure of genomic DNA was unique, and numerous stress-response cis-elements were found in the promoter region. Expression of cDNA EgProT1 in Escherichia coli mutant exhibited uptake activities for glycinebetaine and choline as well as proline. Under salt-stressed conditions, exogenously applied glycinebetaine was taken up into the root more rapidly than the control. These data indicate that oil palm has a unique Pro/T1 gene. Nucleotide sequence data for the cDNA and genomic DNA of proline transporter gene from Elaeis guineensis are available in the DDJB database under accession numbers AB597035 and AB597036, respectively.
authors.affiliation: Graduate School of Environmental and Human Sciences, Meijo University, Nagoya 468-8502, Japan.
authors.forename: Nana
authors.initials: N
authors.lastname: Yamada
authors.forename: Suriyan
authors.initials: S
authors.lastname: Cha-Um
authors.forename: Hakuto
authors.initials: H
authors.lastname: Kageyama
authors.forename: Worrawat
authors.initials: W
authors.lastname: Promden
authors.forename: Yoshito
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Chalermpol
authors.initials: C
authors.lastname: Kirdmanee
authors.forename: Teruhiro
authors.initials: T
authors.lastname: Takabe
mesh_terms: D026921:Amino Acid Transport Systems, Neutral / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D028023:Arecaceae / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D001622:Betaine / Q000032:analysis / Q000378:metabolism*; D001692:Biological Transport; D002352:Carrier Proteins / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D002794:Choline / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D050485:GABA Plasma Membrane Transport Proteins; D049452:Green Fluorescent Proteins; D008969:Molecular Sequence Data; D010802:Phylogeny; D011392:Proline / Q000032:analysis / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics; D012639:Seeds / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological
chemical_list: D026921:Amino Acid Transport Systems, Neutral; D002352:Carrier Proteins; D018076:DNA, Complementary; D018744:DNA, Plant; D050485:GABA Plasma Membrane Transport Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; C072207:betaine plasma membrane transport proteins; D049452:Green Fluorescent Proteins; D001622:Betaine; D012965:Sodium Chloride; C033082:proline transporter; D011392:Proline; D002794:Choline