title: Construction of recombinant lentivirus vector for tumor vasoinhibitory peptide alphastatin gene delivery.
abstract: Angiogenesis is a prerequisite for tumor progression and metastasis. Alphastatin, as an endogenous angiogenesis inhibitor, was recently used as an anticancer agent in several tumor models. We constructed recombinant self-inactivating lentivirus vectors expressing alphastatin and evaluated their ability to transfer genes into human umbilical vein endothelial cells (HUVECs) as well as their antiangiogenic activities in vitro. Recombinant self-inactivating lentiviral vectors efficiently and stably transduced endothelial cells, and lentivirus-transduced HUVECs were capable of sustainedly secreting the antiangiogenesis peptide alphastatin. Long-term expression and secretion of alphastatin resulted in significant inhibition of endothelial cell angiogenesis induced by vascular endothelial growth factor. This report presents the first use of lentivirus-based vectors to deliver the endogenous angiogenesis inhibitor alphastatin, and suggests the potential utility of antiangiogenic gene therapy with lentiviral vectors for the treatment of cancer.
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital, Medical School of Xi'an Jiaotong University, Shaanxi 710061, P.R. China.
authors.forename: S W
authors.initials: SW
authors.lastname: Guo
authors.forename: H M
authors.initials: HM
authors.lastname: Che
authors.forename: W Z
authors.initials: WZ
authors.lastname: Li