title: Fabrication of three-dimensional collagen scaffold using an inverse mould-leaching process.
abstract: Natural biopolymers, such as collagen or chitosan, are considered ideal for biomedical scaffolds. However, low processability of the materials has hindered the fabrication of designed pore structures controlled by various solid freeform-fabrication methods. A new technique to fabricate a biomedical three-dimensional collagen scaffold, supplemented with a sacrificial poly(ethylene oxide) mould is proposed. The fabricated collagen scaffold shows a highly porous surface and a three-dimensional structure with high porosity as well as mechanically stable structure. To show its feasibility for biomedical applications, fibroblasts/keratinocytes were co-cultured on the scaffold, and the cell proliferation and cell migration of the scaffold was more favorable than that obtained with a spongy-type collagen scaffold.
authors.affiliation: Bio/Nanofluidics Laboratory, Department of Mechanical Engineering, Chosun University, 375 Seosok-dong, Gwangju, 501-759, Korea.
authors.forename: SeungHyun
authors.initials: S
authors.lastname: Ahn
authors.forename: SuYeon
authors.initials: S
authors.lastname: Lee
authors.forename: Youngseok
authors.initials: Y
authors.lastname: Cho
authors.forename: Wook
authors.initials: W
authors.lastname: Chun
authors.forename: GeunHyung
authors.initials: G
authors.lastname: Kim
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D049109:Cell Proliferation; D002478:Cells, Cultured; D018920:Coculture Techniques / Q000379:methods; D003094:Collagen / Q000737:chemistry* / Q000648:ultrastructure; D005240:Feasibility Studies; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism*; D008422:Materials Testing; D011092:Polyethylene Glycols / Q000737:chemistry*; D016062:Porosity; D012038:Regeneration; D012867:Skin; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D011092:Polyethylene Glycols; D003094:Collagen