title: [Effect of ginsenoside Rb1 on immune maturation of human monocyte-derived dendritic cells induced by oxidized low-density lipoprotein].
abstract: OBJECTIVE
  To examine the effect of ginsenoside Rb1 (GRb1) on the immune maturation of monocyte-derived dendritic cells (DCs) induced by oxidized low-density lipoprotein (OX-LDL).
  
  
  METHODS
  Human monocytes purified by CD14+ immuno-magnetic beads were differentiated and induced into immature DCs, which were randomly divided into 6 groups, Group A treated with PBS, Group B treated with OX-LDL, Group C and D treated respectively with GRb1 and ciglitazone, Group E and F were pretreated with the two testing drugs respectively followed by OX-LDL. The immuno-phenotypic expression (CD40, CD1a, and HLA-DR) and endocytosis function of DCs were examined using flow cytometry, the concentration of interleukin-12 (IL-12) and tumor necrosis factor alpha (TNF-alpha) in the culture supernatants were measured with ELISA.
  
  
  RESULTS
  Compared with Group B, Group E showed significantly lowered immuno-phenotypic expression of DCs in terms of CD40 (67.4 +/- 1.62 vs. 145.69 +/- 14.86), CD1a (79.64 +/- 3.04 vs. 159.89 +/- 6.09), and HLA-DR (46.43 +/- 2.85 vs. 99.33 +/- 17.11), as well as higher endocytosis level (88.13% +/- 1.06% vs. 25.90% +/- 5.77%, all P < 0.01). Meantime, the serum levels of IL-12 (88.65 +/- 5.59 ng/L vs. 716.69 +/- 36.35 ng/L) and TNF-alpha (133.27 +/- 11.98 ng/L vs. 968.10 +/- 36.42 ng/L) obviously decreased (P < 0.01). The surface molecular expression of DCs and the secretion of inflammatory factors in Group F also obviously decreased, showing insignificant difference from Group E (P > 0.05).
  
  
  CONCLUSIONS
  GRb1 could obviously inhibit the OX-LDL-induced maturation of DCs, showing similar effects to ciglitazone.
authors.affiliation: Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing.
authors.forename: Hong-ying
authors.initials: HY
authors.lastname: Liu
authors.forename: Da-zhuo
authors.initials: DZ
authors.lastname: Shi
authors.forename: Jun-bo
authors.initials: JB
authors.lastname: Ge
mesh_terms: D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D005434:Flow Cytometry; D036145:Ginsenosides / Q000494:pharmacology*; D006801:Humans; D008077:Lipoproteins, LDL; D009000:Monocytes / Q000166:cytology / Q000187:drug effects* / Q000276:immunology
chemical_list: D036145:Ginsenosides; D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein; C442759:ginsenoside Rb1