title: [Transformation system of Aspergillus japonicus mediated by Agrobacterium tumefaciens].
abstract: OBJECTIVE
  In to order to select Aspergillus japonicus mutant strains that express high-yield of glycerol oxidase, we constructed a mutant library of Aspergillus japonicus that mediated by Agrobacterium tumefaciens.
  
  
  METHODS
  Tri-relative hybridization experiments were performed to transfer binary vector pB I -hph II into Agrobacterium tumefaciens EHA105 that was used as the infective strain and Aspergillus japonicus was used as the host strain. We established the transformation system of Aspergillus japonicus mediated by Agrobacterium tumefaciens and constructed a mutant library. At the same time, we analyzed the factors affecting the transformation efficiency such as the concentration of Agrobacterium tumefaciens, adding and not adding Acetosyringone, co-culture time, and co-culture temperature.
  
  
  RESULTS
  PCR detection and Southern blot analysis showed that the T-DNA was integrated into genme of the Aspergillus japonicus. All 9 randomly selected transformants were stable after 10 rounds of successive cultures.
  
  
  CONCLUSIONS
  The transformation system is a good basis for selection of Aspergillus japonicus mutant strains that express high-yield of glycerol oxidase.
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Department of Microbiology, Nankai University, Tianjin 300071, China. guohui204@yahoo.com.cn
authors.forename: Hui
authors.initials: H
authors.lastname: Guo
authors.forename: Zhe
authors.initials: Z
authors.lastname: Yang
authors.forename: Laijun
authors.initials: L
authors.lastname: Xing
authors.forename: Mingchun
authors.initials: M
authors.lastname: Li
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics* / Q000502:physiology; D001230:Aspergillus / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D014170:Transformation, Genetic
chemical_list: D004269:DNA, Bacterial; C036483:T-DNA