title: Synthesis and evaluation of a bimodal CXCR4 antagonistic peptide.
abstract: The antagonistic Ac-TZ14011 peptide, which binds to the chemokine receptor 4, has been labeled with a multifunctional single attachment point reagent that contains a DTPA chelate and a fluorescent dye with Cy5.5 spectral properties. Flow cytometry and confocal microscopy showed that the bimodal labeled peptide gave a specific receptor binding that is similar to monofunctionalized peptide derivatives. Therefore, the newly developed bimodal peptide derivative can be used in multimodal imaging applications.
authors.affiliation: Division of Diagnostic Oncology, The Netherlands Cancer Institute , Antoni van Leeuwenhoek Hospital, Amsterdam, The Netherlands.
authors.forename: Joeri
authors.initials: J
authors.lastname: Kuil
authors.forename: Tessa
authors.initials: T
authors.lastname: Buckle
authors.forename: Hushan
authors.initials: H
authors.lastname: Yuan
authors.forename: Nynke S
authors.initials: NS
authors.lastname: van den Berg
authors.forename: Shinya
authors.initials: S
authors.lastname: Oishi
authors.forename: Nobutaka
authors.initials: N
authors.lastname: Fujii
authors.forename: Lee
authors.initials: L
authors.lastname: Josephson
authors.forename: Fijs W B
authors.initials: FW
authors.lastname: van Leeuwen
mesh_terms: D000818:Animals; D002614:Chelating Agents / Q000737:chemistry*; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D051379:Mice; D018613:Microscopy, Confocal; D008968:Molecular Conformation; D004369:Pentetic Acid / Q000737:chemistry*; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D019718:Receptors, CXCR4 / Q000037:antagonists & inhibitors*
chemical_list: D002614:Chelating Agents; D005456:Fluorescent Dyes; D010455:Peptides; D019718:Receptors, CXCR4; D004369:Pentetic Acid