title: Gas chromatography/mass spectrometry based hair steroid profiling may reveal pathogenesis in hair follicles of the scalp.
abstract: A method of steroid profiling, including androgens, progestins, corticoids and sterols, was developed to evaluate the concentrations of steroids as well as the activities of the enzymes responsible for steroidogenesis in hair by gas chromatography/mass spectrometry. The extraction efficiencies of steroids from the hair matrix were improved by ultrasonication for 1 h at 50 °C. The overall recoveries ranged from 71 to 132%, with a limit of quantification for all analytes ranging from 1 to 50 ng/g. The devised method was used to identify the metabolic changes for both male-pattern baldness (MPB) and the drug efficiency of dutasteride, which inhibits 5α-reductase. Increased dihydrotestosterone levels and the dihydrotestosterone/testosterone (DHT/T) ratio, which is responsible for the 5α-reductase activity, were observed in the MPB patients. A dutasteride treatment resulted in decreases in the DHT and 5α-androstanedione concentrations and DHT/T ratio in the hair samples. Hair steroid profiling reflects the sebaceous status in the scalp and may be useful for monitoring the metabolic responses to both the disease and drug actions.
authors.affiliation: Life/Health Division, Korean Institute of Science and Technology, Seoul, Korea.
authors.forename: Hyun-Jin
authors.initials: HJ
authors.lastname: Jung
authors.forename: Sun Ju
authors.initials: SJ
authors.lastname: Kim
authors.forename: Won-Yong
authors.initials: WY
authors.lastname: Lee
authors.forename: Bong Chul
authors.initials: BC
authors.lastname: Chung
authors.forename: Man Ho
authors.initials: MH
authors.lastname: Choi
mesh_terms: D058891:5-alpha Reductase Inhibitors / Q000627:therapeutic use; D000505:Alopecia / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D001378:Azasteroids / Q000627:therapeutic use; D015415:Biomarkers / Q000032:analysis; D013196:Dihydrotestosterone / Q000032:analysis; D000068538:Dutasteride; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006197:Hair / Q000737:chemistry*; D018859:Hair Follicle / Q000737:chemistry* / Q000378:metabolism / Q000473:pathology; D006358:Hot Temperature; D006801:Humans; D008297:Male; D000432:Methanol / Q000737:chemistry; D015203:Reproducibility of Results; D012535:Scalp; D052616:Solid Phase Extraction; D013010:Sonication; D013256:Steroids / Q000032:analysis*; D013739:Testosterone / Q000032:analysis
chemical_list: D058891:5-alpha Reductase Inhibitors; D001378:Azasteroids; D015415:Biomarkers; D013256:Steroids; D013196:Dihydrotestosterone; D013739:Testosterone; D000068538:Dutasteride; D000432:Methanol