title: A parallel microfluidic flow cytometer for high-content screening.
abstract: A parallel microfluidic cytometer (PMC) uses a high-speed scanning photomultiplier-based detector to combine low-pixel-count, one-dimensional imaging with flow cytometry. The 384 parallel flow channels of the PMC decouple count rate from signal-to-noise ratio. Using six-pixel one-dimensional images, we investigated protein localization in a yeast model for human protein misfolding diseases and demonstrated the feasibility of a nuclear-translocation assay in Chinese hamster ovary (CHO) cells expressing an NFκB-EGFP reporter.
authors.affiliation: Boston University, Department of Biomedical Engineering, Boston, Massachusetts, USA.
authors.forename: Brian K
authors.initials: BK
authors.lastname: McKenna
authors.forename: James G
authors.initials: JG
authors.lastname: Evans
authors.forename: Man Ching
authors.initials: MC
authors.lastname: Cheung
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Ehrlich
mesh_terms: D021581:Active Transport, Cell Nucleus; D000465:Algorithms; D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D005434:Flow Cytometry / Q000295:instrumentation* / Q000706:statistics & numerical data; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D057166:High-Throughput Screening Assays / Q000295:instrumentation / Q000706:statistics & numerical data; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000706:statistics & numerical data; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000706:statistics & numerical data; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D055503:Protein Multimerization; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D051844:alpha-Synuclein / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D016328:NF-kappa B; D011993:Recombinant Fusion Proteins; D051844:alpha-Synuclein; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins