title: Bacterial dynamics in a raw cow's milk Caciotta cheese manufactured with aqueous extract of Cynara cardunculus dried flowers.
abstract: OBJECTIVE
  To investigate the bacterial dynamics of a Caciotta cheese traditionally manufactured in the Montefeltro area (Central Italy) with raw cow's milk and an aqueous extract of dried flowers from Cynara cardunculus as a coagulating agent.
  
  
  RESULTS
  Conventional methods and a combined PCR-DGGE approach, relying on culture-dependent and -independent analyses, were used to investigate the cheese bacterial community, with a special focus on lactic acid bacteria. A heterogeneous population, including enterococci, lactococci, lactobacilli, food spoilage and other banal micro-organisms, was found.
  
  
  CONCLUSIONS
  The study contributed to highlighting the influence of different technological parameters on bacterial dynamics of a raw milk Caciotta cheese coagulated with vegetable rennet.
  
  
  CONCLUSIONS
  None of the species found in the vegetable rennet became dominant during the cheese-making and a prevailing role of the adventitions microbita coming from the raw milk and the dairy environment was highlighted.
authors.affiliation: Sezione di Microbiologia Alimentare, Ambientale ed Industriale, SAIFET, Università Politecnica delle Marche, Ancona, Italy. l.aquilanti@univpm.it
authors.forename: L
authors.initials: L
authors.lastname: Aquilanti
authors.forename: V
authors.initials: V
authors.lastname: Babini
authors.forename: S
authors.initials: S
authors.lastname: Santarelli
authors.forename: A
authors.initials: A
authors.lastname: Osimani
authors.forename: A
authors.initials: A
authors.lastname: Petruzzelli
authors.forename: F
authors.initials: F
authors.lastname: Clementi
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification*; D002417:Cattle; D002611:Cheese / Q000382:microbiology*; D031942:Cynara; D058645:Denaturing Gradient Gel Electrophoresis / Q000379:methods; D035264:Flowers; D007558:Italy; D007778:Lactobacillus / Q000254:growth & development / Q000302:isolation & purification; D017045:Lactococcus / Q000254:growth & development / Q000302:isolation & purification; D008892:Milk / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods