title: Characterization of monoclonal antibodies to the plasma cell alloantigen ENPP1.
abstract: The ectonucleotide pyrophosphatase phosphodiesterase 1 (ENPP1) has documented roles in mineralization, nucleotide recycling, and insulin resistance. While ENPP1 was first identified as an alloantigen on mouse plasma cells (PCs), later studies revealed expression in many tissues. Previously described monoclonal antibodies against ENPP1 expressed at the cell surface recognized cells only from mice bearing the a allotype, ENPP1(a), precluding studies of mice bearing the alternative allele, ENPP1(b). Here, we characterize a novel anti-ENPP1 monoclonal antibody that recognizes both alleles and can be used for flow cytometry.
authors.affiliation: Laboratory of Immunopathology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 5640 Fishers Lane, Rockville, MD 20850, USA.
authors.forename: Sadia
authors.initials: S
authors.lastname: Abbasi
authors.forename: Dong-Mi
authors.initials: DM
authors.lastname: Shin
authors.forename: Natalie
authors.initials: N
authors.lastname: Beaty
authors.forename: Marek
authors.initials: M
authors.lastname: Masiuk
authors.forename: Sophia
authors.initials: S
authors.lastname: Chen
authors.forename: Ines
authors.initials: I
authors.lastname: Gonzalez-Garcia
authors.forename: Ming
authors.initials: M
authors.lastname: Zhao
authors.forename: James
authors.initials: J
authors.lastname: Goding
authors.forename: Herbert C
authors.initials: HC
authors.lastname: Morse
authors.forename: Hongsheng
authors.initials: H
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology*; D002853:Chromatography, Liquid; D005434:Flow Cytometry / Q000379:methods*; D007126:Immunoglobulin Allotypes / Q000276:immunology; D047468:Immunoprecipitation; D007519:Isoantigens / Q000276:immunology*; D013058:Mass Spectrometry; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D010727:Phosphoric Diester Hydrolases / Q000235:genetics / Q000276:immunology*; D011755:Pyrophosphatases / Q000235:genetics / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D007126:Immunoglobulin Allotypes; D007519:Isoantigens; D010727:Phosphoric Diester Hydrolases; C065962:ectonucleotide pyrophosphatase phosphodiesterase 1; D011755:Pyrophosphatases