title: Preparation and analysis of glycan microarrays.
abstract: Determination of the binding specificity of glycan-binding proteins (GBPs), such as lectins, antibodies, and receptors, has traditionally been difficult and laborious. The advent of glycan microarrays has revolutionized the field of glycobiology by allowing simultaneous screening of a GBP for interactions with a large set of glycans in a single format. This unit describes the theory and method for production of two types of glycan microarrays (chemo/enzymatically synthesized and naturally derived), and their application to functional glycomics to explore glycan recognition by GBPs. These procedures are amenable to various types of arrays and a wide range of GBP samples.
authors.affiliation: The Glycomics Center at Emory University School of Medicine, Atlanta, Georgia, USA.
authors.forename: Jamie
authors.initials: J
authors.lastname: Heimburg-Molinaro
authors.forename: Xuezheng
authors.initials: X
authors.lastname: Song
authors.forename: David F
authors.initials: DF
authors.lastname: Smith
authors.forename: Richard D
authors.initials: RD
authors.lastname: Cummings
mesh_terms: D054794:Glycomics / Q000379:methods*; D037102:Lectins; D046228:Microarray Analysis / Q000379:methods*; D008958:Models, Molecular; D011134:Polysaccharides / Q000032:analysis*
chemical_list: D037102:Lectins; D011134:Polysaccharides