title: Alterations in the expression of genes related to NF-κB signaling in liver and kidney of CuZnSOD-deficient mice.
abstract: NF-κB signaling pathway plays a central role in regulation of the cellular response to stress. Among numerous factors that modulate NF-κB dependent transcription, reactive oxygen species attracted special attention. In the present work, we compared the expression of 84 genes related to NF-κB signaling between cytosolic superoxide dismutase (CuZnSOD)-deficient and wild-type mice. In kidney, we found seven genes which expression was significantly affected by CuZnSOD deficiency. Among them, four were up-regulated, Egr1, Fos, Il1b, Tnfrsf10b, and three down-regulated, Card10, Ikbkb, Tgfbr2. In the case of liver, six genes were up-regulated, Fos, Il1b, Il1r1, Jun, Tlr7, Tnfrsf10b, and five down-regulated, Casp8, Ikbke, Irak1, Nfkb1, Raf1. The results demonstrate that CuZnSOD deficiency has a significant impact on the expression of NF-κB related genes in both kidney and liver. The differences in gene expression reported in our work may contribute to understanding of the molecular mechanisms underlying phenotypic abnormalities in CuZnSOD-deficient mice, e.g., increase in the incidence of liver cancer.
authors.affiliation: Centre for Radiobiology and Biological Dosimetry, Institute of Nuclear Chemistry and Technology, Dorodna 16, 03-195 Warsaw, Poland. k.brzoska@ichtj.waw.pl
authors.forename: Kamil
authors.initials: K
authors.lastname: Brzóska
authors.forename: Barbara
authors.initials: B
authors.lastname: Sochanowicz
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Siomek
authors.forename: Ryszard
authors.initials: R
authors.lastname: Oliński
authors.forename: Marcin
authors.initials: M
authors.lastname: Kruszewski
mesh_terms: D000818:Animals; D053468:CARD Signaling Adaptor Proteins / Q000235:genetics; D053181:Caspase 8 / Q000235:genetics; D015536:Down-Regulation / Q000235:genetics; D051766:Early Growth Response Protein 1 / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D007668:Kidney / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D051379:Mice; D057507:Mice, 129 Strain; D018345:Mice, Knockout; D016328:NF-kappa B / Q000235:genetics*; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000235:genetics*; D013482:Superoxide Dismutase / Q000172:deficiency / Q000235:genetics*; D015854:Up-Regulation / Q000235:genetics
chemical_list: D053468:CARD Signaling Adaptor Proteins; C498975:Card10 protein, mouse; D051766:Early Growth Response Protein 1; C486109:Egr1 protein, mouse; D016328:NF-kappa B; D016760:Proto-Oncogene Proteins c-fos; D013482:Superoxide Dismutase; C505526:Casp8 protein, mouse; D053181:Caspase 8