title: [Establishment of PD-L1 transgenic mouse model and recovery of the motion after spinal cord injury].
abstract: OBJECTIVE
  To establish a ubiquitously expressed PD-L1 transgenic mouse model and evaluate its recovery of motor function after spinal cord injury.
  
  
  METHODS
  Clone and sequence the complete mouse PD-L1 cDNA and construct the pCAG-PD-L1 transgenic vector by inserting the PD-L1 cDNA into the pCAGGS vector. The PD-L1 transgenic (TgPD-L1) mice were established by pronuclear micro-injection with fertilized eggs from C57BL/6 mice and the genotypes were confirmed by PCR with tail genomic DNA. The expression of PD-L1 on T and B lymphocytes from mouse spleen were detected by flow cytometry. The expression of PD-L1 in peripheral tissues was displayed by immunohistochemistry. The expression level of PD-L1 in spinal tissue was evaluated by RT-PCR. The recovery of motor function was analyzed by Basso-Beattie-Bresnahan(BBB) locomotion testing system at 3, 7, 14, 21, 28 and 35 day after spinal severe crush with forceps in mice.
  
  
  RESULTS
  Three lines of TgPD-L1 mice in C57BL/6 background were generated and the exogenous PD-L1 gene can be heritable steadily to offsprings. PD-L1 was highly exppressed in spinal tissue, peripheral tissues, T and B lymphocytes using RT-PCR, immunohistochemistry and flow cytometry respectively in TgPD-L1 mice. The BBB scores were obviously higher at 21 day post-injury in TgPD-L1 than those of in WT mice (P<0.05).
  
  
  CONCLUSIONS
  The TgPD-L1 mice whose background are C57BL/6 were established successfully and high expression level of PD-L1 in tissues promotes locomotion recovery after spinal cord injury in TgPD-L1 mice.
authors.affiliation: Department of Neurobiology, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Tang
authors.forename: Cai-yong
authors.initials: CY
authors.lastname: Yu
authors.forename: Rui
authors.initials: R
authors.lastname: Feng
authors.forename: Fang-fang
authors.initials: FF
authors.lastname: Liu
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhao
authors.forename: Hong-min
authors.initials: HM
authors.lastname: Guo
authors.forename: Gong
authors.initials: G
authors.lastname: Ju
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D018122:B7-1 Antigen / Q000032:analysis / Q000235:genetics* / Q000502:physiology; D060890:B7-H1 Antigen; D004195:Disease Models, Animal; D005260:Female; D008124:Locomotion; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis / Q000235:genetics* / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D008822:Mice, Transgenic; D010455:Peptides / Q000032:analysis / Q000235:genetics* / Q000502:physiology; D013119:Spinal Cord Injuries / Q000503:physiopathology*
chemical_list: D018122:B7-1 Antigen; D060890:B7-H1 Antigen; C498919:Cd274 protein, mouse; D008562:Membrane Glycoproteins; D010455:Peptides