title: The stromal cell antigen CD248 (endosialin) is expressed on naive CD8+ human T cells and regulates proliferation.
abstract: CD248 (endosialin) is a transmembrane glycoprotein that is dynamically expressed on pericytes and fibroblasts during tissue development, tumour neovascularization and inflammation. Its role in tissue remodelling is associated with increased stromal cell proliferation and migration. We show that CD248 is also uniquely expressed by human, but not mouse (C57BL/6), CD8(+) naive T cells. CD248 is found only on CD8(+) CCR7(+) CD11a(low) naive T cells and on CD8 single-positive T cells in the thymus. Transfection of the CD248 negative T-cell line MOLT-4 with CD248 cDNA surprisingly reduced cell proliferation. Knock-down of CD248 on naive CD8 T cells increased cell proliferation. These data demonstrate opposing functions for CD248 on haematopoietic (CD8(+)) versus stromal cells and suggests that CD248 helps to maintain naive CD8(+) human T cells in a quiescent state.
authors.affiliation: Rheumatology Research Group, MRC Centre for Immune Regulation, Institute for Biomedical Research, University of Birmingham, Edgbaston, UK.
authors.forename: Debbie L
authors.initials: DL
authors.lastname: Hardie
authors.forename: Mathew J
authors.initials: MJ
authors.lastname: Baldwin
authors.forename: Amy
authors.initials: A
authors.lastname: Naylor
authors.forename: Oliver J
authors.initials: OJ
authors.lastname: Haworth
authors.forename: Tie Zheng
authors.initials: TZ
authors.lastname: Hou
authors.forename: Sian
authors.initials: S
authors.lastname: Lax
authors.forename: S John
authors.initials: SJ
authors.lastname: Curnow
authors.forename: Nick
authors.initials: N
authors.lastname: Willcox
authors.forename: John
authors.initials: J
authors.lastname: MacFadyen
authors.forename: Clare M
authors.initials: CM
authors.lastname: Isacke
authors.forename: Christopher D
authors.initials: CD
authors.lastname: Buckley
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism*; D000951:Antigens, Neoplasm / Q000378:metabolism*; D015153:Blotting, Western; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D049109:Cell Proliferation; D005434:Flow Cytometry; D006801:Humans; D051379:Mice; D017154:Stromal Cells / Q000276:immunology*
chemical_list: D015703:Antigens, CD; D000951:Antigens, Neoplasm; C078203:CD248 protein, human