title: A small fluorophore reporter of protein conformation and redox state.
abstract: A new thiol-specific reagent introduces a small bis(methylamino)terephthalic acid fluorophore into proteins. The noninvasive probe with distinct spectroscopic properties offers many advantages for protein labeling, purification, and mechanistic work promising to serve as a powerful tool in studies of protein folding and heme redox reactions.
authors.affiliation: Department of Chemistry, Dartmouth College, Hanover, NH 03755, USA.
authors.forename: Graham J
authors.initials: GJ
authors.lastname: Pound
authors.forename: Alexandre A
authors.initials: AA
authors.lastname: Pletnev
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Fang
authors.forename: Ekaterina V
authors.initials: EV
authors.lastname: Pletneva
mesh_terms: D045304:Cytochromes c / Q000737:chemistry; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry*; D006418:Heme / Q000737:chemistry; D007700:Kinetics; D010084:Oxidation-Reduction; D010795:Phthalic Acids / Q000737:chemistry; D017510:Protein Folding; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry; D016596:Vinculin / Q000737:chemistry
chemical_list: C014059:CYC1 protein, S cerevisiae; D005456:Fluorescent Dyes; D010795:Phthalic Acids; D011506:Proteins; D029701:Saccharomyces cerevisiae Proteins; D016596:Vinculin; D006418:Heme; C011363:terephthalic acid; D045304:Cytochromes c