title: [Gentiana veitchiorum particles inhibited LPS induced pulmonary alveolar macrophages (AM) TNF-α production and the underlying mechanism].
abstract: OBJECTIVE
  To investigate the effect of Gentiana veitchiorum particles on the expression of TNF-α in pulmonary alveolar macrophages (AM) which induced by LPS, to explain the mechanism about anti-inflammatory action of Gentiana veitchiorum particles.
  
  
  METHODS
  Purification of rat AM, TNF-α level in AM culture supernatant was detected by ELISA. Western blot method for detecting the expression of TNF-α and pERK in the AM. While application of ERK antagonist (PD98059) in rat AM and the expression of TNF-α was observed by Western blot.
  
  
  RESULTS
  Gentiana veitchiorum particles can reduce the LPS induced AM TNF-α increase in dose dependent manner. Gentiana veitchiorum particles (100 mg/L) can significantly reduce the LPS induced pERK and TNF-α protein expression in AM. compared with LPS stimulation group, we found that ERK inhibitor (PD98059 30 mol/L), Gentiana veitchiorum particles intervention and Gentiana veitchiorum particles+PD98059 groups' TNF-α expression were significantly reduced in rat AM.
  
  
  CONCLUSIONS
  Gentiana veitchiorum particles can inhibit the LPS induced pulmonary AM TNF-α expression, one of the possible mechanism is to inhibit the extracellular signal transduction pathway.
authors.affiliation: Department of Pharmacology, School of Pharmacy, Fourth Military Medical University, Xi'an 710032, China. litao-hy@163.com.cn
authors.forename: Ying
authors.initials: Y
authors.lastname: Hou
authors.forename: Wei
authors.initials: W
authors.lastname: Cao
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.forename: Shui-bing
authors.initials: SB
authors.lastname: Liu
authors.forename: Xiao-nan
authors.initials: XN
authors.lastname: Zhang
authors.forename: Xu-bo
authors.initials: XB
authors.lastname: Li
authors.forename: Qiong
authors.initials: Q
authors.lastname: Tian
authors.forename: Fu-sheng
authors.initials: FS
authors.lastname: You
mesh_terms: D000818:Animals; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D030012:Gentiana; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors*; D020935:MAP Kinase Signaling System / Q000502:physiology; D016676:Macrophages, Alveolar / Q000187:drug effects* / Q000276:immunology; D008297:Male; D010766:Phosphorylation; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
chemical_list: D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D048049:Extracellular Signal-Regulated MAP Kinases