title: Compensation-free, all-fiber-optic, two-photon endomicroscopy at 1.55 μm.
abstract: We present an all-fiber-optic scanning multiphoton endomicroscope with 1.55 μm excitation without the need for prechirping femtosecond pulses before the endomicroscope. The system consists of a 1.55 μm femtosecond fiber laser, a customized double-clad fiber for light delivery and fluorescence collection, and a piezoelectric scan head. We demonstrate two-photon imaging of cultured cells and mouse tissue, both labeled with indocyanine green. Free-space multiphoton imaging with near-IR emission has previously shown benefits in reduced background fluorescence and lower attenuation for the fluorescence emission. For fiber-optic multiphoton imaging there is the additional advantage of using the soliton effect at the telecommunication wavelengths (1.3-1.6 μm) in fibers, permitting dispersion-compensation-free, small-footprint systems. We expect these advantages will help transition multiphoton endomicroscopy to the clinic.
authors.affiliation: Department of Biomedical Engineering, Johns Hopkins University, 720 Rutland Avenue, Baltimore, Maryland 21205, USA.
authors.forename: Kartikeya
authors.initials: K
authors.lastname: Murari
authors.forename: Yuying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Shenping
authors.initials: S
authors.lastname: Li
authors.forename: Yongping
authors.initials: Y
authors.lastname: Chen
authors.forename: Ming-Jun
authors.initials: MJ
authors.lastname: Li
authors.forename: Xingde
authors.initials: X
authors.lastname: Li
mesh_terms: D000818:Animals; D001647:Bile Acids and Salts / Q000378:metabolism; D002460:Cell Line; D007259:Infrared Rays; D051379:Mice; D008853:Microscopy / Q000295:instrumentation*; D055100:Optical Fibers; D017785:Photons
chemical_list: D001647:Bile Acids and Salts