title: Effects of surface dehydration on mucosal wave amplitude and frequency in excised canine larynges.
abstract: OBJECTIVE
  Evaluate the effect of vocal fold surface dehydration on mucosal wave amplitude and frequency.
  
  
  METHODS
  Controlled test-retest.
  
  
  METHODS
  Larynges were mounted on an excised larynx phonation system and attached to a pseudolung in a triple-walled sound-attenuated room that eliminated background noise and maintained a stabilized room temperature and humidity level.
  
  
  METHODS
  High-speed video was recorded for 8 excised canine larynges during exposure to dehumidified air at 20 cm H(2)O. Control trials consisted of high-speed videos recorded for 2 excised canine larynges during exposure to humidified air at the same pressure.
  
  
  RESULTS
  In the majority of larynges, increased levels of dehydration were correlated with decreased amplitude and frequency. The slope of the linear regression fitted to the change in amplitude (P = .003) and the percent change (P < .001) between the initial and final trials were significantly decreased in dehydrated larynges. These measurements with respect to the change in frequency were also significantly decreased in dehydrated larynges (P < .001; P = .027).
  
  
  CONCLUSIONS
  Vocal fold surface dehydration caused a decrease in mucosal wave amplitude and frequency. This study provides objective, quantitative support for the mechanism of voice deterioration observed after extreme surface dehydration.
authors.affiliation: University of Wisconsin-Madison School of Medicine and Public Health, Department of Surgery, Division of Otolaryngology-Head and Neck Surgery, Madison, WI 53706, USA.
authors.forename: Rachel E
authors.initials: RE
authors.lastname: Witt
authors.forename: Lindsay N
authors.initials: LN
authors.lastname: Taylor
authors.forename: Michael F
authors.initials: MF
authors.lastname: Regner
authors.forename: Jack J
authors.initials: JJ
authors.lastname: Jiang
mesh_terms: D000818:Animals; D003681:Dehydration; D004285:Dogs; D007820:Laryngeal Mucosa / Q000502:physiology*; D007825:Laryngectomy; D007830:Larynx / Q000166:cytology* / Q000601:surgery; D009092:Mucous Membrane / Q000166:cytology; D010699:Phonation / Q000502:physiology*; D014827:Vocal Cords / Q000502:physiology*