title: OVCA1 inhibits the proliferation of epithelial ovarian cancer cells by decreasing cyclin D1 and increasing p16.
abstract: OVCA1, a tumor suppressor gene, is deleted or lower expressed in about 80% of ovarian cancer. Over expression of OVCA1 in human ovarian cancer A2780 cells inhibits cell proliferation and arrests cells in G1 stage. However, the fact that the molecular mechanism of OVCA1 inhibits cell growth is presently elusive. Here we investigated the potential signaling pathway induced by over-expression of OVCA1. Our results show that over-expression of human OVCA1 in ovarian cancer cells A2780 leads to down-regulation of cyclin D1, and up-regulation of p16, but no effect on the expression of NF-κB. It indicates that OVCA1 could inhibit the proliferation of ovarian cancer cell A2780 by p16/cyclin D1 pathway, but not by NF-κB.
authors.affiliation: Department of Gynecology and Obstetrics, The First Affiliated Hospital, Dalian Medical University, Dalian, China.
authors.forename: Fandou
authors.initials: F
authors.lastname: Kong
authors.forename: Rui
authors.initials: R
authors.lastname: Tong
authors.forename: Lingyu
authors.initials: L
authors.lastname: Jia
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Miao
authors.forename: Xinyu
authors.initials: X
authors.lastname: Zhao
authors.forename: Wenping
authors.initials: W
authors.lastname: Sun
authors.forename: Guang
authors.initials: G
authors.lastname: Yang
authors.forename: Chunyan
authors.initials: C
authors.lastname: Zhao
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation; D019938:Cyclin D1 / Q000235:genetics / Q000378:metabolism*; D019941:Cyclin-Dependent Kinase Inhibitor p16; D005260:Female; D016193:G1 Phase; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D015778:Minor Histocompatibility Antigens; D016328:NF-kappa B / Q000378:metabolism; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D010051:Ovarian Neoplasms; D011993:Recombinant Fusion Proteins / Q000378:metabolism*; D014158:Transcription, Genetic; D025521:Tumor Suppressor Proteins / Q000378:metabolism*
chemical_list: C530104:CCND1 protein, human; C000614131:CDKN2A protein, human; D019941:Cyclin-Dependent Kinase Inhibitor p16; C121987:DPH1 protein, human; D015778:Minor Histocompatibility Antigens; D016328:NF-kappa B; D009363:Neoplasm Proteins; D011993:Recombinant Fusion Proteins; D025521:Tumor Suppressor Proteins; D019938:Cyclin D1