title: Jasmonate modulates endocytosis and plasma membrane accumulation of the Arabidopsis PIN2 protein.
abstract: The subcellular distribution of the PIN-FORMED (PIN) family of auxin transporters plays a critical role in auxin gradient-mediated developmental processes, including lateral root formation and gravitropic growth. Here, we report two distinct aspects of CORONATINE INSENSITIVE 1 (COI1)- and AUXIN RESISTANT 1 (AXR1)-dependent methyl jasmonate (MeJA) effects on PIN2 subcellular distribution: at lower concentration (5 μM), MeJA inhibits PIN2 endocytosis, whereas, at higher concentration (50 μM), MeJA reduces PIN2 accumulation in the plasma membrane. We show that mutations of ASA1 (ANTHRANILATE SYNTHASE a1) and the TIR1/AFBs (TRANSPORT INHIBITOR RESPONSE 1/AUXIN-SIGNALING F-BOX PROTEINs) auxin receptor genes impair the inhibitory effect of 5 μM MeJA on PIN2 endocytosis, suggesting that a lower concentration of jasmonate inhibits PIN2 endocytosis through interaction with the auxin pathway. In contrast, mutations of ASA1 and the TIR1/AFBs auxin receptor genes enhance, rather than impair, the reduction effect of 50 μM MeJA on the plasma membrane accumulation of PIN2, suggesting that this action of jasmonate is independent of the auxin pathway. In addition to the MeJA effects on PIN2 endocytosis and plasma membrane residence, we also show that MeJA alters lateral auxin redistribution on gravi-stimulation, and therefore impairs the root gravitropic response. Our results highlight the importance of jasmonate-auxin interaction in the coordination of plant growth and the adaptation response.
authors.affiliation: State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Datun Road, Beijing 100101, China.|Chinese-German Joint Group for Plant Hormone Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Datun Road, Beijing 100101, China.
authors.forename: Jiaqiang
authors.initials: J
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Datun Road, Beijing 100101, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Datun Road, Beijing 100101, China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Qi
authors.affiliation: State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Datun Road, Beijing 100101, China.
authors.forename: Hongling
authors.initials: H
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Datun Road, Beijing 100101, China.
authors.forename: Shuyu
authors.initials: S
authors.lastname: Li
authors.affiliation: State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Datun Road, Beijing 100101, China.
authors.forename: Yingxiu
authors.initials: Y
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Datun Road, Beijing 100101, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Datun Road, Beijing 100101, China.
authors.forename: Wenkun
authors.initials: W
authors.lastname: Zhou
authors.affiliation: College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China.
authors.forename: Jianwei
authors.initials: J
authors.lastname: Pan
authors.affiliation: Chinese-German Joint Group for Plant Hormone Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Datun Road, Beijing 100101, China.|Institute for Biology II/Botany and Freiburg Institute of Advanced Sciences, Faculty of Biology, University of Freiburg, D-79104 Freiburg, Germany.
authors.forename: Xugang
authors.initials: X
authors.lastname: Li
authors.affiliation: Chinese-German Joint Group for Plant Hormone Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Datun Road, Beijing 100101, China.|Institute for Biology II/Botany and Freiburg Institute of Advanced Sciences, Faculty of Biology, University of Freiburg, D-79104 Freiburg, Germany.
authors.forename: Klaus
authors.initials: K
authors.lastname: Palme
authors.affiliation: State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Datun Road, Beijing 100101, China.|Chinese-German Joint Group for Plant Hormone Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Datun Road, Beijing 100101, China.
authors.forename: Chuanyou
authors.initials: C
authors.lastname: Li
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D000878:Anthranilate Synthase / Q000235:genetics; D017360:Arabidopsis / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D001692:Biological Transport / Q000187:drug effects; D002462:Cell Membrane / Q000378:metabolism; D003517:Cyclopentanes / Q000494:pharmacology*; D015536:Down-Regulation; D004705:Endocytosis / Q000187:drug effects*; D044783:F-Box Proteins / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D018522:Gravitropism / Q000187:drug effects; D007210:Indoleacetic Acids / Q000378:metabolism; D018519:Meristem / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D009154:Mutation; D054883:Oxylipins / Q000494:pharmacology*; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000235:genetics; D011993:Recombinant Fusion Proteins; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D000085:Acetates; D000878:Anthranilate Synthase; D029681:Arabidopsis Proteins; D003517:Cyclopentanes; D044783:F-Box Proteins; D007210:Indoleacetic Acids; D054883:Oxylipins; D010937:Plant Growth Regulators; D011956:Receptors, Cell Surface; D011993:Recombinant Fusion Proteins; C081977:AXR1 protein, Arabidopsis; C112279:COI1 protein, Arabidopsis; C117013:AT5G57090 protein, Arabidopsis; C501405:TIR1 protein, Arabidopsis; C072239:methyl jasmonate; C543688:ASA1 protein, Arabidopsis