title: Development of a direct competitive enzyme-linked immunosorbent assay using a sensitive monoclonal antibody for bisphenol A.
abstract: To set up an immunoassay-based method to detect Bisphenol A (BPA), we generated a monoclonal antibody (MAb) using a specially designed carboxyl derivative of BPA as the immunogen. BPA-HS was synthesized by reaction using BPA and succinic anhydride. The mice were immunized with the BPA-HS-BSA conjugate. The MAb was obtained from a hybridoma. In addition, we showed that the MAb was highly specific for BPA. The limit of detection was approximately 0.05 ng mL(-1) (ppb) in assay buffer and 0.1 ng mL(-1) (ppb) in water samples. The recoveries of BPA for water samples were from 90.8% to 114%, and coefficients of variation were from 15.6% to 39.4%. Thus, the ELISA method is a rapid and high throughput screening tool to detect BPA in water products.
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
authors.forename: Chunmei
authors.initials: C
authors.lastname: Ju
authors.forename: Youhua
authors.initials: Y
authors.lastname: Xiong
authors.forename: Aizhong
authors.initials: A
authors.lastname: Gao
authors.forename: Tangbin
authors.initials: T
authors.lastname: Yang
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001559:Benzhydryl Compounds; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D057230:Limit of Detection; D051379:Mice; D008807:Mice, Inbred BALB C; D010636:Phenols / Q000737:chemistry / Q000276:immunology*; D019064:Product Packaging; D012680:Sensitivity and Specificity; D013387:Succinic Anhydrides / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000032:analysis*; D000098826:Bisphenol A Compounds
chemical_list: D000911:Antibodies, Monoclonal; D001559:Benzhydryl Compounds; D010636:Phenols; D013387:Succinic Anhydrides; D014874:Water Pollutants, Chemical; C031801:succinic anhydride; C006780:bisphenol A; D000098826:Bisphenol A Compounds