title: Improved antigen yield in pandemic H1N1 (2009) candidate vaccine viruses with chimeric hemagglutinin molecules.
abstract: The candidate pandemic H1N1 vaccine virus NIBRG-121 was derived by reverse genetics and comprises the hemagglutinin (HA) and neuraminidase (NA) genes from A/California/7/2009 (CAL) on an A/Puerto Rico/8/34 (PR8) backbone. NIBRG-121 was found to grow poorly in eggs, compared to seasonal H1N1 candidate vaccine viruses. Based on our previous study with H5N1 candidate vaccine viruses, we generated two new viruses with chimeric PR8/CAL HA genes. Here we show that these new viruses have considerably improved growth in eggs and are therefore better candidate vaccine viruses for use in production of pandemic H1N1 (2009) vaccine.
authors.affiliation: Division of Virology, National Institute for Biological Standards and Control, Health Protection Agency, Blanche Lane, Potters Bar, Hertfordshire EN6 3QG, United Kingdom.
authors.forename: Ruth
authors.initials: R
authors.lastname: Harvey
authors.forename: Kate A
authors.initials: KA
authors.lastname: Guilfoyle
authors.forename: Sarah
authors.initials: S
authors.lastname: Roseby
authors.forename: James S
authors.initials: JS
authors.lastname: Robertson
authors.forename: Othmar G
authors.initials: OG
authors.lastname: Engelhardt
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002642:Chick Embryo; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000254:growth & development* / Q000276:immunology; D009980:Influenza A virus / Q000235:genetics* / Q000378:metabolism; D007252:Influenza Vaccines; D007251:Influenza, Human / Q000517:prevention & control; D008969:Molecular Sequence Data; D009439:Neuraminidase / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D058873:Pandemics; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism*
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007252:Influenza Vaccines; D011994:Recombinant Proteins; D009439:Neuraminidase