title: Efficacy of marigold extract-loaded formulations against UV-induced oxidative stress.
abstract: The present study investigated the potential use of topical formulations containing marigold extract (ME) (Calendula officinalis extract) against ultraviolet (UV)B irradiation-induced skin damage. The physical and functional stabilities, as well as the skin penetration capacity, of the different topical formulations developed were evaluated. In addition, the in vivo capacity to prevent/treat the UVB irradiation-induced skin damage, in hairless mice, of the formulation with better skin penetration capacity was investigated. All of the formulations were physically and functionally stable. The gel formulation [Formulation 3 (F3)] was the most effective for the topical delivery of ME, which was detected as 0.21 μg/cm(2) of narcissin and as 0.07 μg/cm(2) of the rutin in the viable epidermis. This formulation was able to maintain glutathione reduced levels close to those of nonirradiated animals, but did not affect the gelatinase-9 and myeloperoxidase activities increased by exposure to UVB irradiation. In addition, F3 reduced the histological skin changes induced by UVB irradiation that appear as modifications of collagen fibrils. Therefore, the photoprotective effect in hairless mice achieved with the topical application of ME in gel formulation is most likely associated with a possible improvement in the collagen synthesis in the subepidermal connective tissue.
authors.affiliation: Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Yris Maria
authors.initials: YM
authors.lastname: Fonseca
authors.forename: Carolina Dias
authors.initials: CD
authors.lastname: Catini
authors.forename: Fabiana T M C
authors.initials: FT
authors.lastname: Vicentini
authors.forename: Juliana Cordeiro
authors.initials: JC
authors.lastname: Cardoso
authors.forename: Ricardo Luiz
authors.initials: RL
authors.lastname: Cavalcanti De Albuquerque Junior
authors.forename: Maria José
authors.initials: MJ
authors.lastname: Vieira Fonseca
mesh_terms: D000287:Administration, Topical; D000818:Animals; D020838:Calendula / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D003872:Dermatitis / Q000209:etiology / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control; D004355:Drug Stability; D005782:Gels; D066298:In Vitro Techniques; D051379:Mice; D008812:Mice, Hairless; D018384:Oxidative Stress / Q000187:drug effects* / Q000528:radiation effects*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000493:pharmacokinetics / Q000494:pharmacology*; D012867:Skin / Q000187:drug effects / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects; D012869:Skin Absorption; D013473:Sunscreening Agents / Q000302:isolation & purification / Q000493:pharmacokinetics / Q000494:pharmacology*; D013552:Swine; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D005782:Gels; D010936:Plant Extracts; D013473:Sunscreening Agents