title: [Protective effect of intranasal cilostazol administration on chronic injury after cerebral ischemia in mice].
abstract: OBJECTIVE
  To investigate the protective effect of cilostazol administrated intranasally on chronic injury after focal cerebral ischemia in mice.
  
  
  METHODS
  Focal cerebral ischemia in mice was induced by middle cerebral artery occlusion (MCAO). Cilostazol was administrated intranasally or intraperitoneally 1 h, 4 h and 7 h after the operation; then twice a day from the second day for 2 weeks. The neurological deficit scoring and the inclined board testing were performed within 35 d after ischemia. The survival rate, infarct volume and neuron density were assessed 35 d after ischemia.
  
  
  RESULTS
  Intranasal cilostazol at 0.3 mg/kg increased the survival rate. Intranasal cilostazol (0.3 mg/kg, 1 mg/kg) and intraperitoneal cilostazol (10 mg/kg) significantly attenuated neurological deficit, reduced infarct volume, and increased the survival neuron density in the border of ischemia region.
  
  
  CONCLUSIONS
  Cilostazol administered intranasally demonstrates protective effects on chronic cerebral ischemia in mice.
authors.affiliation: Department of Pharmacology, Zhejiang Medical College, Hangzhou 310053, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.forename: Yi-Lu
authors.initials: YL
authors.lastname: Ye
authors.forename: Qing
authors.initials: Q
authors.lastname: Li
authors.forename: Wei-Ping
authors.initials: WP
authors.lastname: Zhang
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Jiang
authors.forename: Er-Qing
authors.initials: EQ
authors.lastname: Wei
authors.forename: Yue-Ping
authors.initials: YP
authors.lastname: Yu
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000473:pathology; D002545:Brain Ischemia / Q000188:drug therapy* / Q000473:pathology; D000077407:Cilostazol; D004195:Disease Models, Animal; D020244:Infarction, Middle Cerebral Artery / Q000188:drug therapy / Q000473:pathology; D008297:Male; D051379:Mice; D009474:Neurons / Q000187:drug effects / Q000473:pathology; D013777:Tetrazoles / Q000008:administration & dosage* / Q000627:therapeutic use
chemical_list: D013777:Tetrazoles; D000077407:Cilostazol