title: Cloning and characterization of a cDNA encoding type 1 diacylglycerol acyltransferase from sunflower (Helianthus annuus L.).
abstract: A full-length cDNA encoding a putative diacylglycerol acyltransferase (DGAT; EC 2.3.1.20) was obtained from sunflower (Helianthus annuus L.) seeds. The 1524-bp open reading frame of this cDNA, designated as HaDGAT1, encodes a protein of 507 amino acids with a molecular mass of 58.5 kDa showing high homology to DGAT1 enzymes of other plants. The protein characters, such as a predicted structure with a long N-terminal hydrophilic domain followed by 9 transmembrane domains, acyl-CoA-binding signature, diacylglycerol (DAG)-binding and putative endoplasmic reticulum retrieval motifs (ER-DIR), also indicated that HaDGAT belongs to the DGAT1 family. HaDGAT1 is expressed in all plant tissues especially in developing seeds. Expression of recombinant HaDGAT1 in yeast showed an 1.76-fold increase of total fatty acids, especially unsaturated fatty acids such as palmitoleic acid (enhanced by 86.6%) and oleic acid (enhanced by 81.6%).
authors.affiliation: College of Life Science, Sichuan University, 29 Wangjiang Road, 610064 Chengdu, Sichuan, P. R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Sun
authors.forename: Chao
authors.initials: C
authors.lastname: Ouyang
authors.forename: Shanglong
authors.initials: S
authors.lastname: Kou
authors.forename: Shenghua
authors.initials: S
authors.lastname: Wang
authors.forename: Yunyi
authors.initials: Y
authors.lastname: Yao
authors.forename: Tong
authors.initials: T
authors.lastname: Peng
authors.forename: Ying
authors.initials: Y
authors.lastname: Xu
authors.forename: Lin
authors.initials: L
authors.lastname: Tang
authors.forename: Fang
authors.initials: F
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics*; D051048:Diacylglycerol O-Acyltransferase / Q000737:chemistry / Q000235:genetics*; D008401:Gas Chromatography-Mass Spectrometry; D006368:Helianthus / Q000201:enzymology*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D051048:Diacylglycerol O-Acyltransferase