title: [Role of microRNA-223 and its target gene oncogene c-myc in hepatocellular carcinoma pathogenesis].
abstract: To investigate the regulatory role of microRNA-223 (miR-223) on c-myc and its role in hepatocarcinogenesis. miR-223 and c-myc mRNA expressions in normal tissue, paraneoplastic tissue, liver cancer tissue and liver cancer cells were tested with microRNA microarray and quantitative real-time PCR (qRT-PCR). C-myc protein expression was detected by Western blot. MiR-223 mimic was transfected into HepG2 cells and the expression changes of c-myc mRNA and protein were tested with qRT-PCR and Western blot respectively. MiR-223 was down-regulated by 61.53% and 30.77% respectively in hepatocellular carcinoma and adjacent tissues as compared to normal liver tissues and the expression of miR-223 was also decreased in HepG2 cell as compared to fetal liver cells L02, whereas the expressions of c-myc mRNA and protein increased in paraneoplastic and HCC tissues compared with normal liver tissues. It prompts that the expressions of miR-223 and c-myc are negatively correlated. No obvious difference found among c-myc mRNA expressions after miR-223 mimics transfection. The c-myc abnormal high-expression may play a dynamic role in hepatocarcinogenesis due to the miR-223 down-regulation.
authors.affiliation: Digested Sections of Union Hospital of Huazhong University of Science and Technology, Wuhan 430022, China.
authors.forename: Wen-Yue
authors.initials: WY
authors.lastname: Zhao
authors.forename: Dong-Dong
authors.initials: DD
authors.lastname: Wang
authors.forename: Meng-Qi
authors.initials: MQ
authors.lastname: Song
authors.forename: Ling
authors.initials: L
authors.lastname: Yang
authors.forename: Jin
authors.initials: J
authors.lastname: Ye
authors.forename: Li-Bo
authors.initials: LB
authors.lastname: Chen