title: Peripheral nervous system progenitors can be reprogrammed to produce myelinating oligodendrocytes and repair brain lesions.
abstract: Neural crest stem cells (NCSCs) give rise to the neurons and glia of the peripheral nervous system (PNS). NCSC-like cells can be isolated from multiple peripheral organs and maintained in neurosphere culture. Combining in vitro culture and transplantation, we show that expanded embryonic NCSC-like cells lose PNS traits and are reprogrammed to generate CNS cell types. When transplanted into the embryonic or adult mouse CNS, they differentiate predominantly into cells of the oligodendrocyte lineage without any signs of tumor formation. NCSC-derived oligodendrocytes generate CNS myelin and contribute to the repair of the myelin deficiency in shiverer mice. These results demonstrate a reprogramming of PNS progenitors to CNS fates without genetic modification and imply that PNS cells could be a potential source for cell-based CNS therapy.
authors.affiliation: Department of Neurochemistry, Max Planck Institute for Brain Research, D-60528 Frankfurt/Main, Germany.
authors.forename: Ellen
authors.initials: E
authors.lastname: Binder
authors.forename: Marion
authors.initials: M
authors.lastname: Rukavina
authors.forename: Hessameh
authors.initials: H
authors.lastname: Hassani
authors.forename: Marlen
authors.initials: M
authors.lastname: Weber
authors.forename: Hiroko
authors.initials: H
authors.lastname: Nakatani
authors.forename: Tobias
authors.initials: T
authors.lastname: Reiff
authors.forename: Carlos
authors.initials: C
authors.lastname: Parras
authors.forename: Verdon
authors.initials: V
authors.lastname: Taylor
authors.forename: Hermann
authors.initials: H
authors.lastname: Rohrer
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D051792:Basic Helix-Loop-Helix Proteins / Q000378:metabolism; D001930:Brain Injuries / Q000378:metabolism / Q000503:physiopathology / Q000601:surgery*; D002454:Cell Differentiation / Q000502:physiology; D002465:Cell Movement / Q000187:drug effects / Q000235:genetics; D002478:Cells, Cultured; D004195:Disease Models, Animal; D004622:Embryo, Mammalian; D005260:Female; D005727:Ganglia, Spinal / Q000166:cytology; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D004676:Myelin Basic Protein / Q000235:genetics / Q000378:metabolism; D018991:Myelin Proteolipid Protein / Q000235:genetics / Q000378:metabolism; D009186:Myelin Sheath / Q000378:metabolism* / Q000648:ultrastructure; D009419:Nerve Tissue Proteins / Q000378:metabolism; D058953:Neural Stem Cells / Q000502:physiology*; D016900:Neurofilament Proteins / Q000378:metabolism; D019081:O Antigens / Q000378:metabolism; D000076104:Oligodendrocyte Transcription Factor 2; D009836:Oligodendroglia / Q000502:physiology* / Q000648:ultrastructure; D033581:Stem Cell Transplantation / Q000379:methods*; D014162:Transfection / Q000379:methods; D014404:Tubulin / Q000378:metabolism
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D005904:Glial Fibrillary Acidic Protein; D049452:Green Fluorescent Proteins; D036341:Intercellular Signaling Peptides and Proteins; D008869:Microtubule-Associated Proteins; D004676:Myelin Basic Protein; D018991:Myelin Proteolipid Protein; D009419:Nerve Tissue Proteins; D016900:Neurofilament Proteins; D019081:O Antigens; D000076104:Oligodendrocyte Transcription Factor 2; D014404:Tubulin; C079240:Mtap2 protein, mouse; C512773:Olig2 protein, mouse; C047516:Plp1 protein, mouse; C462941:beta3 tubulin, mouse; C485184:enhanced green fluorescent protein