title: The stochastic quasi-steady-state assumption: reducing the model but not the noise.
abstract: Highly reactive species at small copy numbers play an important role in many biological reaction networks. We have described previously how these species can be removed from reaction networks using stochastic quasi-steady-state singular perturbation analysis (sQSPA). In this paper we apply sQSPA to three published biological models: the pap operon regulation, a biochemical oscillator, and an intracellular viral infection. These examples demonstrate three different potential benefits of sQSPA. First, rare state probabilities can be accurately estimated from simulation. Second, the method typically results in fewer and better scaled parameters that can be more readily estimated from experiments. Finally, the simulation time can be significantly reduced without sacrificing the accuracy of the solution.
authors.affiliation: Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, Wisconsin 53706-1607, USA. rsrivastava2@wisc.edu
authors.forename: Rishi
authors.initials: R
authors.lastname: Srivastava
authors.forename: Eric L
authors.initials: EL
authors.lastname: Haseltine
authors.forename: Ethan
authors.initials: E
authors.lastname: Mastny
authors.forename: James B
authors.initials: JB
authors.lastname: Rawlings
mesh_terms: D053263:Gene Regulatory Networks; D008954:Models, Biological; D009876:Operon / Q000235:genetics; D010507:Periodicity; D013269:Stochastic Processes; D018116:Vesiculovirus / Q000235:genetics / Q000472:pathogenicity