title: Transplantation of skin grafts and organs infected with Toxoplasma gondii as a source of toxoplasmosis in immunocompromised mice.
abstract: The possibility of Toxoplasma gondii infection resulting from transplantation of a skin graft and various organs has been investigated. The parasite was detected in very low numbers in all organs examined in wild-type (WT) BALB/c (B/c) mice that received skin grafts from infected interferon gamma knockout (GKO) B/c mice both with and without sulfamethoxazole treatment; all recipient mice survived. In contrast, transplantation of skin grafts from untreated infected WT B/c mice to naïve GKO B/c mice led to the death of all recipients within 20 days post-transplantation; T. gondii was found to be disseminated in all organs examined. Similar results were obtained after transplantation of skin from untreated and treated GKO B/c mice to naïve GKO B/c mice, whereas the recipient GKO B/c mice died within 10 days after intraperitoneal transplantation of lung, heart, brain or small intestine from infected untreated GKO B/c mice. These results indicate that skin grafts as well as various organs infected with T. gondii can be sources of infection in immunocompromised hosts. Toxoplasmosis should therefore be taken into consideration during organ transplantation to immunocompromised hosts.
authors.affiliation: Department of Infection and Host Defense, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan.
authors.forename: Usama Salah
authors.initials: US
authors.lastname: Belal
authors.forename: Kazumi
authors.initials: K
authors.lastname: Norose
authors.forename: Rabie Mohamed
authors.initials: RM
authors.lastname: Mohamed
authors.forename: Koji
authors.initials: K
authors.lastname: Naoi
authors.forename: Akihiko
authors.initials: A
authors.lastname: Yano
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D016867:Immunocompromised Host; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D016377:Organ Transplantation / Q000009:adverse effects*; D014122:Toxoplasma / Q000302:isolation & purification*; D014123:Toxoplasmosis / Q000175:diagnosis* / Q000635:transmission*; D019737:Transplants / Q000469:parasitology*