title: Chemical modification and structural analysis of protein isolates to produce hydrogel using Whitemouth croaker (Micropogonias furnieri) wastes.
abstract: Recovery and alteration of fish protein from wastes and its use has been regarded as a promising alternative to develop useful products once polymer gels have a high capacity of water uptake. This study aims to produce hydrogel, a super absorbent biopolymer from modified fish protein, in order to evaluate the protein structure. In the modified proteins, analyses of the extent of modification of the lysine residues, electrophoresis, and electrometric titration were performed. In the hydrogels were realized assays of swelling water. The proteins with more modifications were shown as 63.5% and 75.9% of lysine residues, from fish protein isolate obtained with alkaline and acid solubilization, respectively. The modified protein in that same rate presented 332.0 and 311.4 carboxyl groups. Accordingly, the hydrogel produced from alkaline and acid isolates reached a maximum water uptake in 24 h of 79.42 and 103.25 g(water)/g(dry gel), respectively.
authors.affiliation: Laboratory of Food Technology, School of Chemistry and Food, Federal University of Rio Grande, Rio Grande, RS, Brazil.
authors.forename: Vilásia Guimarães
authors.initials: VG
authors.lastname: Martins
authors.forename: Jorge Alberto Vieira
authors.initials: JA
authors.lastname: Costa
authors.forename: Srinivasan
authors.initials: S
authors.lastname: Damodaran
authors.forename: Carlos
authors.initials: C
authors.lastname: Prentice
mesh_terms: D000818:Animals; D001704:Biopolymers / Q000737:chemistry / Q000378:metabolism; D029941:Fish Proteins / Q000737:chemistry* / Q000378:metabolism*; D005976:Glutaral / Q000737:chemistry; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D010473:Perciformes / Q000378:metabolism*
chemical_list: D001704:Biopolymers; D029941:Fish Proteins; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D005976:Glutaral