title: Expression of insulin-like growth factor-1 by canine insulinomas and their metastases.
abstract: The long-term prognosis after surgical resection of canine insulinoma is poor. Signs of hypoglycaemia often recur soon after surgery because tumour tissue has only been resected partially and/or functional (micro-)metastases were present. Using quantitative real-time PCR, the expression of 16 target genes was compared between primary canine insulinomas and their corresponding metastases. There was significantly higher expression of genes encoding for growth hormone (GH) and insulin-like growth factor-1 (IGF-1) in metastases compared to their primary tumours. Immunohistochemical examination of proteins of the GH-IGF-1 axis revealed expression of GH, IGF-1 and GH receptor (GHR) in both primary insulinomas and metastases. Immunohistochemical staining for IGF-1 was significantly higher in metastases compared to primary tumours.
authors.affiliation: Department of Clinical Sciences of Companion Animals, Utrecht University, PO Box 80154, 3508 TD Utrecht, The Netherlands.
authors.forename: Floryne O
authors.initials: FO
authors.lastname: Buishand
authors.forename: Mariëlle G M
authors.initials: MG
authors.lastname: van Erp
authors.forename: Harmke A
authors.initials: HA
authors.lastname: Groenveld
authors.forename: Jan A
authors.initials: JA
authors.lastname: Mol
authors.forename: Marja
authors.initials: M
authors.lastname: Kik
authors.forename: Joris H
authors.initials: JH
authors.lastname: Robben
authors.forename: Hans S
authors.initials: HS
authors.lastname: Kooistra
authors.forename: Jolle
authors.initials: J
authors.lastname: Kirpensteijn
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000378:metabolism*; D004285:Dogs; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D007328:Insulin / Q000235:genetics / Q000378:metabolism; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000378:metabolism*; D007340:Insulinoma / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D053605:Leukemia Inhibitory Factor / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D053606:Receptors, OSM-LIF / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D007328:Insulin; D053605:Leukemia Inhibitory Factor; D053606:Receptors, OSM-LIF; D014157:Transcription Factors; D007334:Insulin-Like Growth Factor I