title: Soluble fms-like tyrosine kinase-1 antibody for diagnosis purposes (WO2010075475).
abstract: BACKGROUND
  The application (WO2010075475) is involved in the diagnosis of angiogenesis-dependent diseases related with the soluble FMS-like tyrosine kinase-1 (sFlt-1) biomarker.
  
  
  OBJECTIVE
  It aims to identify, characterize and produce antibodies raised against sFlt-1 for the diagnosis of preeclampsia as well as other cardiovascular diseases.
  
  
  METHODS
  The diagnostic kit is based on a double mAb sandwich assay, comprising of a capture anti-sFlt-1 mAb, grafted onto paramagnetic particles that are able to bind sFlt-1 both in bound and free forms. An acridinium conjugated anti-sFlt-1 antibody, which binds to s-Flt1 on another epitope, serves as a chemioluminescent label.
  
  
  RESULTS
  Using this assay, the total amount of sFlt-1 could be estimated in various biological samples.
  
  
  CONCLUSIONS
  Antibodies against the free and bound forms of sFlt-1 offer new opportunities in the diagnostics of preeclampsia and other angiogenesis-dependent disorders. Furthermore, as demonstrated in this patent, the immunoassay could be automated and is fast and reliable.
authors.affiliation: Laboratoire de Chimie des Biomolécules et de l'Environnement (LCBE), EA 4215, centre de phytopharmacie, Université de Perpignan Via Domitia, 52 avenue P. Alduy, 66860 Perpignan, France.
authors.forename: France
authors.initials: F
authors.lastname: Boyaud
authors.forename: Nicolas
authors.initials: N
authors.lastname: Inguimbert
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002318:Cardiovascular Diseases / Q000175:diagnosis*; D005260:Female; D006801:Humans; D010330:Patents as Topic; D011225:Pre-Eclampsia / Q000175:diagnosis*; D011247:Pregnancy; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000032:analysis / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; C501162:FLT1 protein, human; D040281:Vascular Endothelial Growth Factor Receptor-1