title: Chronic lymphocytic leukemia with t(14;19)(q32;q13) is characterized by atypical morphologic and immunophenotypic features and distinctive genetic features.
abstract: The t(14;19)(q32;q13) involving the IGH@ and BCL3 loci is an infrequent cytogenetic abnormality detected in B-cell malignancies. We describe the clinicopathologic, cytogenetic, and molecular genetic characteristics of 14 cases of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) with t(14;19)(q32;q13). All patients (10 men and 4 women) had lymphocytosis; 10 had lymphadenopathy. Blood and bone marrow lymphocytes were predominantly small, but cytologically and immunophenotypically atypical. In all cases, t(14;19) was found in the neoplastic stem line; it was the sole abnormality in 4. Ten cases showed additional cytogenetic abnormalities, including trisomy 12 in 9 and complex karyotypes in 7. Fluorescence in situ hybridization demonstrated IGH@/BCL3 fusion gene in all cases. In all cases, the IGHV genes were unmutated, but only 7 expressed ZAP70. Seven cases preferentially used IGHV4-39. Our results indicate that t(14;19)(q32;q13) identifies a subset of CLL/SLL with distinctive clinicopathologic and genetic features. Furthermore, t(14;19) may represent an early, possibly primary, genetic event.
authors.affiliation: Dept of Hematopathology, The University of Texas M. D. Anderson Cancer Center, Houston, 77030, USA.
authors.forename: Yang O
authors.initials: YO
authors.lastname: Huh
authors.forename: Carmen D
authors.initials: CD
authors.lastname: Schweighofer
authors.forename: Rhett P
authors.initials: RP
authors.lastname: Ketterling
authors.forename: Ryan A
authors.initials: RA
authors.lastname: Knudson
authors.forename: Francisco
authors.initials: F
authors.lastname: Vega
authors.forename: Ji E
authors.initials: JE
authors.lastname: Kim
authors.forename: Rajyalakshmi
authors.initials: R
authors.lastname: Luthra
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Keating
authors.forename: L Jeffrey
authors.initials: LJ
authors.lastname: Medeiros
authors.forename: Lynne V
authors.initials: LV
authors.lastname: Abruzzo
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001402:B-Lymphocytes / Q000276:immunology / Q000473:pathology*; D001853:Bone Marrow / Q000473:pathology; D048429:Cell Size; D002883:Chromosomes, Human, Pair 14; D002888:Chromosomes, Human, Pair 19; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D017404:In Situ Hybridization, Fluorescence; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics / Q000473:pathology / Q000503:physiopathology; D008218:Lymphocytosis / Q000235:genetics / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009154:Mutation; D014411:Neoplastic Stem Cells / Q000473:pathology; D015514:Oncogene Proteins, Fusion / Q000235:genetics; D014178:Translocation, Genetic; D051746:ZAP-70 Protein-Tyrosine Kinase / Q000235:genetics
chemical_list: D015514:Oncogene Proteins, Fusion; D051746:ZAP-70 Protein-Tyrosine Kinase; C497261:ZAP70 protein, human