title: Analysis of bacterial populations in the environment using two-dimensional gel electrophoresis of genomic DNA and complementary DNA.
abstract: Two-dimensional gel electrophoresis (2-DGE) mapping of genomic DNA and complementary DNA (cDNA) amplicons was attempted to analyze total and active bacterial populations within soil and activated sludge samples. Distinct differences in the number and species of bacterial populations and those that were metabolically active at the time of sampling were visually observed especially for the soil community. Statistical analyses and sequencing based on the 2-DGE data further revealed the relationships between total and active bacterial populations within each community. This high-resolution technique would be useful for obtaining a better understanding of bacterial population structures in the environment.
authors.affiliation: Graduate School of Engineering, Yokohama National University, Yokohama 240–8501, Japan.
authors.forename: Guo-Hua
authors.initials: GH
authors.lastname: Liu
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Nakamura
authors.forename: Takashi
authors.initials: T
authors.lastname: Amemiya
authors.forename: Narasimmalu
authors.initials: N
authors.lastname: Rajendran
authors.forename: Kiminori
authors.initials: K
authors.lastname: Itoh
mesh_terms: D001419:Bacteria / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D044822:Biodiversity; D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics; D018076:DNA, Complementary / Q000032:analysis / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D004784:Environmental Monitoring / Q000379:methods; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D012722:Sewage / Q000032:analysis / Q000382:microbiology*; D012988:Soil Microbiology; D014197:Trees / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial; D018076:DNA, Complementary; D012336:RNA, Ribosomal, 16S; D012722:Sewage