title: [Metabolic pathways of OGCP and the influence of parkin protein on the metabolism of OGCP].
abstract: OBJECTIVE
  To study the metabolic pathways of 2-oxoglutarate carrier protein (OGCP)and the influence of parkin protein on the metabolism of OGCP.
  
  
  METHODS
  The OGCP metabolic pathways were identified through inhibiting proteasome activities with specific proteasome inhibitors and protease inhibitors. The isotope pulse-chase experiments were performed to measure the turnover rate of OGCP and to study the influence of parkin protein on the metabolism of OGCP.
  
  
  RESULTS
  Proteasome inhibitors and protease inhibitors inhibited OGCP degradation. The OGCP metabolism had a half-life of about 8-10 h. Overexpression of parkin protein accelerated the OGCP degradation.
  
  
  CONCLUSIONS
  OGCP degrades through proteasome and lysosome degradation pathways. The degradation of parkin protein can promote the degradation of OGCP.
authors.affiliation: Department of Neurology, the Second Xiangya Hospital, Central South University, Changsha 410011, China.
authors.forename: Chun-yu
authors.initials: CY
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Cao
authors.forename: Bei-sha
authors.initials: BS
authors.lastname: Tang
authors.forename: Hai-nan
authors.initials: HN
authors.lastname: Zhang
authors.forename: Ji-feng
authors.initials: JF
authors.lastname: Guo
authors.forename: Shu-sheng
authors.initials: SS
authors.lastname: Liao
authors.forename: Jian-guang
authors.initials: JG
authors.lastname: Tang
authors.forename: Xin-riang
authors.initials: XR
authors.lastname: Yan
authors.forename: Li-ming
authors.initials: LM
authors.lastname: Tan
mesh_terms: D006207:Half-Life; D006801:Humans; D008247:Lysosomes / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000378:metabolism*; D053858:Metabolic Networks and Pathways / Q000502:physiology*; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D059748:Proteolysis; D044767:Ubiquitin-Protein Ligases / Q000502:physiology*
chemical_list: D026901:Membrane Transport Proteins; C021723:oxoglutarate translocator; D044767:Ubiquitin-Protein Ligases; C111567:parkin protein; D046988:Proteasome Endopeptidase Complex