title: Effect of mucoprotein on the bond strength of resin composite to human dentin.
abstract: The purpose of this study was to test the bond strength and analyze the morphology of the dentin-adhesive interface of two etch and rinse and two self-etch adhesive systems with two kinds of artificial saliva (with and without 450 mg/L mucin) contamination under different conditions of decontaminating the interface. Bonded specimens were sectioned perpendicularly to the bonded surface in 1-mm thick slabs. These 1-mm thick slabs were remounted in acrylic blocks and sectioned in sticks perpendicular to the bonding interfaces with a 1-mm(2) area. Nine specimens from each condition were tested after 24 h on a testing machine (Instron) at a speed of 0.5 mm/min for a total of 360 specimens. Mean and standard deviations of bond strength (MPa) were calculated. ANOVA showed significant differences as well as Fisher's PLSD intervals (p < 0.05). The following values are the results for different groups: Control group 34-60 MPa, saliva without mucin 0-52 MPa, and saliva with mucin 0-57 MPa. Failure sites were mixed and adhesive failure was common for the low bond strength results. P&BNT with ideal conditions and following the manufacturer's instructions (control) had the highest bond strengths and the dentin-adhesive interface exhibited an ideal morphology of etch-and-rinse system. SEM gave complementary visual evidence of the effect in the dentin/adhesive interface structure with some contaminated conditions compared with their respective control groups. This in vitro artificial saliva model with and without mucin showed that an organic component of saliva could increase or decrease the bond strength depending on the specific bonding agent and decontamination procedure.
authors.affiliation: Department of Preventive and Restorative Dentistry, University of California, San Francisco, 707 Parnassus Avenue Room 3212, San Francisco, CA 94143-0758, USA. Lilliam.Pinzon@ucsf.edu
authors.forename: Lilliam Marie
authors.initials: LM
authors.lastname: Pinzon
authors.forename: John M
authors.initials: JM
authors.lastname: Powers
authors.forename: Kathy L
authors.initials: KL
authors.lastname: O'Keefe
authors.forename: Vladimir
authors.initials: V
authors.lastname: Dusevish
authors.forename: Paulette
authors.initials: P
authors.lastname: Spencer
authors.forename: Grayson W
authors.initials: GW
authors.lastname: Marshall
mesh_terms: D000134:Acid Etching, Dental / Q000379:methods; D000268:Adhesiveness; D017438:Bisphenol A-Glycidyl Methacrylate / Q000737:chemistry; D003188:Composite Resins / Q000737:chemistry*; D001840:Dental Bonding; D003799:Dental Stress Analysis / Q000295:instrumentation; D003804:Dentin / Q000648:ultrastructure*; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D008689:Methacrylates / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D009077:Mucins / Q000737:chemistry*; D055668:Photochemical Processes; D058105:Polymerization; D011109:Polymethacrylic Acids / Q000737:chemistry; D019279:Resin Cements / Q000737:chemistry; D012464:Saliva, Artificial / Q000737:chemistry; D012471:Salivary Proteins and Peptides / Q000737:chemistry*; D013314:Stress, Mechanical; D013499:Surface Properties; D014867:Water / Q000737:chemistry
chemical_list: C112828:Clearfil AP-X; C505694:Clearfil S3 Bond; C438313:Clearfil SE Bond; D003188:Composite Resins; D017220:Dentin-Bonding Agents; C474035:Filtek Supreme; D008689:Methacrylates; D009077:Mucins; D011109:Polymethacrylic Acids; C425843:Prime and Bond NT; D019279:Resin Cements; D012464:Saliva, Artificial; D012471:Salivary Proteins and Peptides; C511238:Single Bond Plus; C467914:TPH spectrum; D014867:Water; D017438:Bisphenol A-Glycidyl Methacrylate