title: Neurogenesis of adipose-derived stem cells in hydrogel.
abstract: Adipose tissue is a readily available source of adult stem cells with multipotent properties suitable for tissue engineering and regenerative medical applications. Peptide hydrogel is a novel biomaterial which provides three-dimensional microenvironments for a variety of cells for tissue grafting. In this study, adipose-derived stem cells (ADSCs) were isolated from rats, seeded into the peptide hydrogel polymer scaffolds and cultured in Neurobasal (NB) media supplemented with B27, basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF). Ten days after the culture, some cells were expanded into clonal populations in which the expression of both Nestin and Brdu was detected but only Brdu expression was detected in the cells that were not expanded into clonal populations. Our results suggested that ADSCs in peptide hydrogel polymer scaffolds can be induced to differentiate into cells capable of expressing the neuron-associated markers, self-renewal and self-propagation.
authors.affiliation: Department of Neurology, Tongji Hospital, Tongji Medical University, Huazhong University of Science and Technology, Wuhan, 430030, China.|Department of Neurology, Changzheng Hospital, the Second Military Medical University, Shanghai, 200003, China.
authors.forename: Xuewei
authors.initials: X
authors.lastname: Xie
authors.affiliation: Department of Neurology, Tongji Hospital, Tongji Medical University, Huazhong University of Science and Technology, Wuhan, 430030, China. ddjtzp@163.com.
authors.forename: Zhouping
authors.initials: Z
authors.lastname: Tang
authors.affiliation: Department of Neurology, Tongji Hospital, Tongji Medical University, Huazhong University of Science and Technology, Wuhan, 430030, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Neurology, Tongji Hospital, Tongji Medical University, Huazhong University of Science and Technology, Wuhan, 430030, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Neurology, Tongji Hospital, Tongji Medical University, Huazhong University of Science and Technology, Wuhan, 430030, China.
authors.forename: Wengao
authors.initials: W
authors.lastname: Zeng
authors.affiliation: Department of Neurology, Tongji Hospital, Tongji Medical University, Huazhong University of Science and Technology, Wuhan, 430030, China.
authors.forename: Na
authors.initials: N
authors.lastname: Liu
authors.affiliation: Department of Neurology, Tongji Hospital, Tongji Medical University, Huazhong University of Science and Technology, Wuhan, 430030, China.
authors.forename: Yongming
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D020100:Hydrogels / Q000737:chemistry*; D008297:Male; D009474:Neurons / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000166:cytology*; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D020100:Hydrogels