title: Crystallization and initial crystallographic analysis of AafA: the major adhesive subunit of the enteroaggregative Escherichia coli AAF/II pilus.
abstract: AafA is the major adhesive pilin subunit of the aggregative adherence fimbriae (AAF) from enteroaggregative Escherichia coli, which play an important role by attaching to the host cells during the initial phase of bacterial colonization and invasion. AafA has been crystallized at pH 3.4 and diffraction data have been collected to 2.1 Å resolution. Molecular replacement was unsuccessful and selenomethionine-substituted protein and heavy-atom derivatives are being prepared for phasing.
authors.affiliation: Centre for Structural Biology, Division of Molecular Biosciences, Department of Life Sciences, Imperial College London, South Kensington, London SW7 2AZ, England.
authors.forename: Yi
authors.initials: Y
authors.lastname: Yang
authors.forename: James A
authors.initials: JA
authors.lastname: Garnett
authors.forename: Stephen
authors.initials: S
authors.lastname: Matthews
mesh_terms: D018830:Adhesins, Escherichia coli / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000737:chemistry*; D010861:Fimbriae, Bacterial / Q000737:chemistry*; D021122:Protein Subunits / Q000737:chemistry
chemical_list: C108511:AafA protein, E coli; D018830:Adhesins, Escherichia coli; D021122:Protein Subunits