title: Cancer of the gastrointestinal tract results in a restricted T-cell repertoire dependent upon tumor differentiation.
abstract: We investigated the influence of tumor tissue differentiation on the diversity of TCR repertoire. CDR3 spectratypes of CD4(+) and CD8(+) T cell subsets were analyzed from 27 patients with gastrointestinal tract tumors exhibiting varying degrees of differentiation. A CDR3 spectratype complexity scoring system was used to quantify the diversity of TCR repertoire. Each patient was matched with an age-matched healthy group to control for age variability. Results show that the complexity scores (TCR repertoire diversity) have a significant correlation with the degree of tumor differentiation, which provides useful information for understanding immune response in cancer patients.
authors.affiliation: Institute of Molecular Immunology, School of Biotechnology, Southern Medical University, Guangzhou 510515, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Luo
authors.forename: Wang-Jun
authors.initials: WJ
authors.lastname: Liao
authors.forename: Yong-Ta
authors.initials: YT
authors.lastname: Huang
authors.forename: Min
authors.initials: M
authors.lastname: Shi
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Qian
authors.initials: Q
authors.lastname: Wen
authors.forename: Ming-Qian
authors.initials: MQ
authors.lastname: Zhou
authors.forename: Li
authors.initials: L
authors.lastname: Ma
mesh_terms: D000328:Adult; D000368:Aged; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002454:Cell Differentiation; D002471:Cell Transformation, Neoplastic; D003110:Colonic Neoplasms / Q000276:immunology* / Q000473:pathology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007111:Immunity, Cellular; D016130:Immunophenotyping; D008297:Male; D008875:Middle Aged; D017260:Receptor-CD3 Complex, Antigen, T-Cell / Q000276:immunology*; D012004:Rectal Neoplasms / Q000276:immunology* / Q000473:pathology*; D013274:Stomach Neoplasms / Q000276:immunology* / Q000473:pathology*; D016176:T-Lymphocyte Subsets / Q000276:immunology
chemical_list: D017260:Receptor-CD3 Complex, Antigen, T-Cell