title: High-purity flow sorting of early meiocytes based on DNA analysis of guinea pig spermatogenic cells.
abstract: Mammalian spermatogenesis is still nowadays poorly understood at the molecular level. Testis cellular heterogeneity is a major drawback for spermatogenic gene expression studies, especially when research is focused on stages that are usually very short and poorly represented at the cellular level such as initial meiotic prophase I (i.e., leptotene [L] and zygotene [Z]). Presumably, genes whose products are involved in critical meiotic events such as alignment, pairing and recombination of homologous chromosomes are expressed during the short stages of early meiotic prophase. Aiming to characterize mammalian early meiotic gene expression, we have found the guinea pig (Cavia porcellus) as an especially attractive model. A detailed analysis of its first spermatogenic wave by flow cytometry (FCM) and optical microscopy showed that guinea pig testes exhibit a higher representation of early meiotic stages compared to other studied rodents, partly because of their longer span, and also as a result of the increased number of cells entering meiosis. Moreover, we have found that adult guinea pig testes exhibit a peculiar 4C DNA content profile, with a bimodal peak for L/Z and P spermatocytes that is absent in other rodents. Besides, we show that this unusual 4C peak allows the separation by FCM of highly pure L/Z spermatocyte populations aside from pachytene ones, even from adult individuals. To our knowledge, this is the first report on an accurate and suitable method for highly pure early meiotic prophase cell isolation from adult mammals, and thus sets an interesting approach for gene expression studies aiming at a deeper understanding of the molecular groundwork underlying male gamete production.
authors.affiliation: Departamento de Biología Molecular, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), Montevideo, Uruguay. rosana@iibce.edu.uy
authors.forename: Rosana
authors.initials: R
authors.lastname: Rodríguez-Casuriaga
authors.forename: Adriana
authors.initials: A
authors.lastname: Geisinger
authors.forename: Federico F
authors.initials: FF
authors.lastname: Santiñaque
authors.forename: Beatriz
authors.initials: B
authors.lastname: López-Carro
authors.forename: Gustavo A
authors.initials: GA
authors.lastname: Folle
mesh_terms: D000818:Animals; D002469:Cell Separation / Q000379:methods*; D004247:DNA / Q000032:analysis*; D005434:Flow Cytometry / Q000379:methods*; D015870:Gene Expression; D006168:Guinea Pigs; D008297:Male; D049469:Meiotic Prophase I / Q000235:genetics; D015877:Miosis / Q000235:genetics*; D013091:Spermatogenesis / Q000235:genetics*; D013737:Testis / Q000166:cytology
chemical_list: D004247:DNA