title: Resistance to tetracycline and β-lactams and distribution of resistance markers in enteric microorganisms and pseudomonads isolated from the oral cavity.
abstract: This study evaluated the occurrence of enteric bacteria and pseudomonads resistant to tetracycline and β-lactams in the oral cavity of patients exhibiting gingivitis (n=89), periodontitis (n=79), periodontally healthy (n=50) and wearing complete dentures (n=41). Microbial identification and presence of resistance markers associated with the production of β-lactamases and tetracycline resistance were performed by using biochemical tests and PCR. Susceptibility tests were carried out in 201 isolates of enteric cocci and rods. Resistance to ampicillin, amoxicillin/clavulanic acid, imipenem, meropenem and tetracycline was detected in 57.4%, 34.6%, 2.4%, 1.9% and 36.5% of the isolates, respectively. β-lactamase production was observed in 41.2% of tested microorganisms, while the most commonly found β-lactamase genetic determinant was gene blaTEM. Tetracycline resistance was disseminated and a wide scope of tet genes were detected in all studied microbial genus.
authors.affiliation: Department of Pathology and Clinical Propedeutics, School of Dentistry of Araçatuba, São Paulo State University.
authors.forename: Marcelle Marie Buso
authors.initials: MM
authors.lastname: Ramos
authors.forename: Ellen Cristina
authors.initials: EC
authors.lastname: Gaetti-Jardim
authors.forename: Elerson
authors.initials: E
authors.lastname: Gaetti-Jardim Junior
mesh_terms: D000328:Adult; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015415:Biomarkers; D003824:Denture, Complete / Q000382:microbiology; D004755:Enterobacteriaceae / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D005798:Genes, Bacterial; D005891:Gingivitis / Q000382:microbiology; D006801:Humans; D008297:Male; D009055:Mouth / Q000382:microbiology*; D010518:Periodontitis / Q000382:microbiology; D016133:Polymerase Chain Reaction; D011549:Pseudomonas / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D013752:Tetracycline / Q000494:pharmacology; D013753:Tetracycline Resistance / Q000235:genetics*; D018440:beta-Lactam Resistance / Q000235:genetics*; D001618:beta-Lactamases / Q000378:metabolism / Q000494:pharmacology; D047090:beta-Lactams / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D015415:Biomarkers; D047090:beta-Lactams; D001618:beta-Lactamases; D013752:Tetracycline