title: Characterization of glycosyltransferases responsible for salidroside biosynthesis in Rhodiola sachalinensis.
abstract: Salidroside, the 8-O-β-D-glucoside of tyrosol, is a novel adaptogenic drug extracted from the medicinal plant Rhodiola sachalinensis A. Bor. Due to the scarcity of R. sachalinensis and its low yield of salidroside, there is great interest in enhancing production of salidroside by biotechnological manipulations. In this study, two putative UDP-glycosyltransferase (UGT) cDNAs, UGT72B14 and UGT74R1, were isolated from roots and cultured cells of methyl jasmonate (MeJA)-treated R. sachalinensis, respectively. The level of sequence identity between their deduced amino acid sequences was ca. 20%. RNA gel-blot analysis established that UGT72B14 transcripts were more abundant in roots, and UGT74R1 was highly expressed in the calli, but not in roots. Functional analysis indicated that recombinant UGT72B14 had the highest level of activity for salidroside production, and that the catalytic efficiency (Vmax/Km) of UGT72B14 was 620% higher than that of UGT74R1. The salidroside contents of the UGT72B14 and UGT74R1 transgenic hairy root lines of R. sachalinensis were also ∼420% and ∼50% higher than the controls, respectively. UGT72B14 transcripts were mainly detected in roots, and UGT72B14 had the highest level of activity for salidroside production in vitro and in vivo.
authors.affiliation: Food Science and Engineering College, Jilin Agricultural University, Changchun 130118, People's Republic of China.
authors.forename: Han-Song
authors.initials: HS
authors.lastname: Yu
authors.affiliation: Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture PR China, Beijing University of Agriculture, Beijing 102206, People's Republic of China.
authors.forename: Lan-Qing
authors.initials: LQ
authors.lastname: Ma
authors.affiliation: College of Life Science, Inner Mongolia University for Nationalities, Tongliao 028043, People's Republic of China.
authors.forename: Ji-Xing
authors.initials: JX
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture PR China, Beijing University of Agriculture, Beijing 102206, People's Republic of China.
authors.forename: Guang-Lu
authors.initials: GL
authors.lastname: Shi
authors.affiliation: Food Science and Engineering College, Jilin Agricultural University, Changchun 130118, People's Republic of China.
authors.forename: Yao-Hui
authors.initials: YH
authors.lastname: Hu
authors.affiliation: Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture PR China, Beijing University of Agriculture, Beijing 102206, People's Republic of China.
authors.forename: You-Nian
authors.initials: YN
authors.lastname: Wang
mesh_terms: D000085:Acetates; D003517:Cyclopentanes; D005960:Glucosides / Q000096:biosynthesis*; D016695:Glycosyltransferases / Q000235:genetics / Q000378:metabolism*; D054883:Oxylipins; D010636:Phenols; D010802:Phylogeny; D018517:Plant Roots / Q000201:enzymology; D030821:Plants, Genetically Modified / Q000201:enzymology; D011994:Recombinant Proteins / Q000378:metabolism; D029771:Rhodiola / Q000201:enzymology* / Q000235:genetics
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D005960:Glucosides; D054883:Oxylipins; D010636:Phenols; D011994:Recombinant Proteins; C072239:methyl jasmonate; D016695:Glycosyltransferases; C009172:rhodioloside