title: Hollow fiber liquid-phase microextraction as clean-up step for the determination of organophosphorus pesticides residues in fish tissue by gas chromatography coupled with mass spectrometry.
abstract: Hollow fiber liquid-phase microextraction (HF-LPME) technique was used as a clean-up procedure for the determination of organophosphorus pesticides (OPPs) in fish tissue. In this study, eight OPPs were first extracted with acetone from fish sample, the organic extract after rotatory evaporation was then redissolved with water-methanol (95:5, v/v) solution, followed by polyvinylidene difluoride (PVDF) HF-LPME. Experimental HF-LPME and other sample preparation conditions were carefully investigated and optimized. Under the optimum conditions, good linearity were observed in the range of 20-500 ng/g, limits of detections (LODs) were in the range of 2.1-4.5 ng/g. The repeatability and recovery of the method also showed satisfactory results. Compared with traditional sample preparation method for the determination of OPPs in fish tissue, the method developed in this study eliminated the solid phase extraction (SPE) step, simplified the sample preparation procedure and lowered the cost of analysis.
authors.affiliation: MOE Key Laboratory of Aquatic Product Safety/KLGHEI of Environment and Energy Chemistry, School of Chemistry and Chemical Engineer, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Xiaojin
authors.initials: X
authors.lastname: Sun
authors.forename: Fang
authors.initials: F
authors.lastname: Zhu
authors.forename: Jiabin
authors.initials: J
authors.lastname: Xi
authors.forename: Tongbu
authors.initials: T
authors.lastname: Lu
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.forename: Yexiang
authors.initials: Y
authors.lastname: Tong
authors.forename: Gangfeng
authors.initials: G
authors.lastname: Ouyang
mesh_terms: D000818:Animals; D004784:Environmental Monitoring / Q000379:methods*; D005399:Fishes / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D057230:Limit of Detection; D009943:Organophosphorus Compounds / Q000737:chemistry / Q000378:metabolism*; D010575:Pesticides / Q000737:chemistry / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000378:metabolism*
chemical_list: D009943:Organophosphorus Compounds; D010575:Pesticides; D014874:Water Pollutants, Chemical