title: Ectonucleoside triphosphate diphosphohydrolase 6 expression in testis and testicular cancer and its implication in cisplatin resistance.
abstract: The development of resistance to cisplatin during treatment of testicular cancer constitutes a major obstacle to the cure of testicular cancer. To resolve its mechanism will provide useful information for making clinical decisions. We found 4 and 15 gene expressions were, respectively, up-regulated and down-regulated in cisplatin-resistant testicular cancer NEC-8/DDP cells compared with their parental NEC-8 cells (about 2.5-fold) using cDNA microarray analysis. After screening, we selected the ENTPD6 among these 19 genes. ENTPD6 was less expressed in cancerous region compared with that in non-cancerous lesion. In addition, ENTPD6 expression in seminoma was higher than that in other testicular tumors. ENTPD6 expression was involved in cellular sensitivity to cisplatin through an interaction with E-cadherin. ENTPD6 is a promising molecular biomarker of cisplatin resistance in testicular cancer, and also a novel therapeutical target modulating cisplatin resistance in testicular cancer.
authors.affiliation: Department of Urology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Tada
authors.forename: Akira
authors.initials: A
authors.lastname: Yokomizo
authors.forename: Masaki
authors.initials: M
authors.lastname: Shiota
authors.forename: Yoohyun
authors.initials: Y
authors.lastname: Song
authors.forename: Eiji
authors.initials: E
authors.lastname: Kashiwagi
authors.forename: Kentaro
authors.initials: K
authors.lastname: Kuroiwa
authors.forename: Yoshinao
authors.initials: Y
authors.lastname: Oda
authors.forename: Seiji
authors.initials: S
authors.lastname: Naito
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D014408:Biomarkers, Tumor; D015820:Cadherins / Q000378:metabolism; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000494:pharmacology*; D019008:Drug Resistance, Neoplasm; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D011755:Pyrophosphatases / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013736:Testicular Neoplasms / Q000188:drug therapy* / Q000201:enzymology; D013737:Testis / Q000201:enzymology*
chemical_list: D000970:Antineoplastic Agents; D014408:Biomarkers, Tumor; D015820:Cadherins; C561876:ENTPD6 protein, human; D011755:Pyrophosphatases; D002945:Cisplatin