title: Characterization of a novel dextran produced by Gluconobacter oxydans DSM 2003.
abstract: A novel water-soluble dextran was synthesized from maltodextrin by cell-free extract of Gluconobacter oxydans DSM 2003. The dextran was purified by size exclusion chromatography, and the structure was determined by Fourier transform infrared spectroscopy, nuclear magnetic resonance, and gas chromatography-mass spectrometer. Based on the spectral data, we found that the dextran contained only D-glucose residues. The ratio of nonreducing end glucopyranosyl (Glcp) to 6-linked Glcp to 4,6-linked Glcp was estimated to be 8.62:78.79:12.59 by methylation analysis. This result indicated the existence of a small proportion of α(1,4) branches in α(1,6) glucosyl linear chains. Here, we reported the first time a novel dextran was synthesized by G. oxydans DSM 2003.
authors.affiliation: Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Wang
authors.forename: Xiangzhao
authors.initials: X
authors.lastname: Mao
authors.forename: Hualei
authors.initials: H
authors.lastname: Wang
authors.forename: Jinping
authors.initials: J
authors.lastname: Lin
authors.forename: Fuli
authors.initials: F
authors.lastname: Li
authors.forename: Dongzhi
authors.initials: D
authors.lastname: Wei
mesh_terms: D001709:Biotechnology; D003911:Dextrans / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification; D008401:Gas Chromatography-Mass Spectrometry; D020600:Gluconobacter oxydans / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D011134:Polysaccharides / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D003911:Dextrans; D011134:Polysaccharides; C008315:maltodextrin