title: 1H, 13C, and 15N resonance assignment of the central domain of hRSV transcription antitermination factor M2-1.
abstract: M2-1 is an essential co-factor of the respiratory syncytial virus, an important respiratory pathogen in infants and calves. It acts as a transcription antitermination factor which enhances the processivity of the polymerase. Within the polymerase complex, M2-1 interacts with a second co-factor, the phosphoprotein P. It has been shown previously that P and RNA bind to M2-1 in a competitive manner in vitro and that these properties are related to a central domain located between residues Glu59 and Lys177. Here we report the almost complete (1)H, (13)C and (15)N assignment of a fragment of M2-1 corresponding to this region, for further structure determination and interaction studies.
authors.affiliation: Unité de Virologie et Immunologie Moléculaires, INRA UR0892, 78350, Jouy-en-Josas, France.
authors.forename: Virginie
authors.initials: V
authors.lastname: Dubosclard
authors.forename: Marie-Lise
authors.initials: ML
authors.lastname: Blondot
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Eléouët
authors.forename: François
authors.initials: F
authors.lastname: Bontems
authors.forename: Christina
authors.initials: C
authors.lastname: Sizun
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D007554:Isotopes / Q000737:chemistry; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D016601:RNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics; D018113:Respiratory Syncytial Virus, Human / Q000737:chemistry* / Q000235:genetics; D016415:Sequence Alignment; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics
chemical_list: D007554:Isotopes; D016601:RNA-Binding Proteins; D014764:Viral Proteins