title: The evolution of Pyrosequencing® for microbiology: From genes to genomes.
abstract: Pyrosequencing is a unique sequencing method that was developed as an alternative to classical DNA sequencing for short- to medium-read applications. Compared to other methods, it is highly quantitative, fast and inexpensive. Additional advantages include high accuracy, flexibility and ability to automate sample preparation. This article presents a historical overview of pyrosequencing, improvements that have been made through the years and its evolution into a platform that vastly expanded the scope of genetic analysis that could be performed outside of a big sequencing center. In addition, we describe numerous applications in microbiology that have benefited from the pyrosequencing method.
authors.affiliation: University of State of Rio de Janeiro, Rio de Janeiro, RJ, Brazil. rnovais@uerj.br
authors.forename: R C
authors.initials: RC
authors.lastname: Novais
authors.forename: Y R
authors.initials: YR
authors.lastname: Thorstenson
mesh_terms: D005796:Genes; D016678:Genome; D008829:Microbiology / Q000295:instrumentation; D017422:Sequence Analysis, DNA / Q000295:instrumentation / Q000379:methods*