title: Detection and characterization of a multicopper oxidase from Nitrosomonas europaea.
abstract: Blue copper oxidase (BCO) is a multicopper oxidase (MCO) found in Nitrosomonas europaea as well as in other ammonia-oxidizing organisms. In this chapter, we detail methods used to detect, isolate, and characterize BCO from N. europaea. A method for identifying and classifying MCOs commonly found in nitrifiers based on primary sequence is also described.
authors.affiliation: Department of Molecular Biosciences, Northwestern University, Evanston, Illinois, USA.
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Lawton
authors.forename: Amy C
authors.initials: AC
authors.lastname: Rosenzweig
mesh_terms: D000641:Ammonia / Q000378:metabolism; D003460:Crystallization; D043370:Nitrosomonas europaea / Q000201:enzymology*; D010088:Oxidoreductases / Q000032:analysis* / Q000737:chemistry* / Q000145:classification; D011487:Protein Conformation
chemical_list: D000641:Ammonia; D010088:Oxidoreductases; C024748:copper oxidase