title: Patulibacter ginsengiterrae sp. nov., isolated from soil of a ginseng field, and an emended description of the genus Patulibacter.
abstract: A novel actinobacterial strain, designated P4-5(T), was isolated from soil of a ginseng field located in Geumsan County, Republic of Korea. Cells of strain P4-5(T) were Gram-stain-positive, oxidase- and catalase-positive, motile, short rods and the strain produced creamy white colonies on trypticase soy agar. The isolate contained demethylmenaquinone 7 (DMK-7) as the predominant isoprenoid quinone, C(18:1)ω9c and anteiso-C(15:0) as major fatty acids, diphosphatidylglycerol, phosphatidylglycerol and several unknown lipids in the polar lipid profile, galactose, glucose, mannose, arabinose, xylose (trace) and rhamnose as cell-wall sugars, and meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan. The DNA G+C content of strain P4-5(T) was 74.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequencing showed that strain P4-5(T) was related most closely to Patulibacter minatonensis KV-614(T) and Patulibacter americanus CP177-2(T) (98.4 and 98.2% similarity, respectively) and that it formed a separate lineage in the genus Patulibacter. Combined phenotypic and DNA-DNA hybridization data supported the conclusion that strain P4-5(T) represents a novel species of the genus Patulibacter, for which the name Patulibacter ginsengiterrae sp. nov. is proposed. The type strain is P4-5(T) (=KCTC 19427(T) =CECT 7603(T)). An emended description of the genus Patulibacter is also provided.
authors.affiliation: Korean Collection for Type Cultures, Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, 52 Eoeun-dong, Yuseong-gu, Daejeon 305-806, Republic of Korea.
authors.forename: Kwang Kyu
authors.initials: KK
authors.lastname: Kim
authors.affiliation: Korean Collection for Type Cultures, Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, 52 Eoeun-dong, Yuseong-gu, Daejeon 305-806, Republic of Korea.
authors.forename: Keun Chul
authors.initials: KC
authors.lastname: Lee
authors.affiliation: Korean Collection for Type Cultures, Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, 52 Eoeun-dong, Yuseong-gu, Daejeon 305-806, Republic of Korea.
authors.forename: Jung-Sook
authors.initials: JS
authors.lastname: Lee
mesh_terms: D039903:Actinobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002241:Carbohydrates / Q000032:analysis; D002473:Cell Wall / Q000737:chemistry; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003960:Diaminopimelic Acid / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D005894:Panax / Q000254:growth & development; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D002241:Carbohydrates; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D003960:Diaminopimelic Acid