title: Comparative salivary proteomics of cleft palate patients.
abstract: OBJECTIVE
  To investigate salivary proteins with proteomic technologies to evaluate protein composition differences between samples with cleft lip and palate and healthy controls.
  
  
  METHODS
  Matrix-assisted laser desorption/ionization tandem time-of-flight (MALDI TOF/TOF) mass spectrometry was used as a high-throughput analytical technique for identification of nonsyndromic cleft lip and palate stimulated salivary proteins. The samples consisted of two groups: 31 cleft lip and palate patients and a control group with 20 healthy volunteers.
  
  
  RESULTS
  The presence of cleft lip and palate stimulated the expression of several proteins, included adaptor-related protein complex 3, dermokine, nidogen 1 precursor, transforming growth factor-β3, and a zinc finger RAN-binding domain containing 2.
  
  
  CONCLUSIONS
  The salivary proteome of cleft lip and palate patients differs from the protein composition of healthy control saliva samples. Several common secreted proteins such as actins, salivary cystatins, and keratins were upregulated by cleft; increased levels of TGF-β3 and dermokine were detected in the pathologic samples. The current proteomic results suggest keratinocyte activation among patients with cleft lip and palate. The score of our preliminary results suggests the hypothesis that identified salivary proteins are of vital clinical importance in tissue regeneration and the molecular repair mechanism seen in patients with cleft lip and palate.
authors.affiliation: Department of Dentistry, Oral and Maxillofacial Surgery, Medical School, University of Pecs, Pecs, Hungary.
authors.forename: Gyula Tamas
authors.initials: GT
authors.lastname: Szabo
authors.forename: Reka
authors.initials: R
authors.lastname: Tihanyi
authors.forename: Fruzsina
authors.initials: F
authors.lastname: Csulak
authors.forename: Eva
authors.initials: E
authors.lastname: Jambor
authors.forename: Agnes
authors.initials: A
authors.lastname: Bona
authors.forename: Gyula
authors.initials: G
authors.lastname: Szabo
authors.forename: Laszlo
authors.initials: L
authors.lastname: Mark
mesh_terms: D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D002971:Cleft Lip / Q000378:metabolism*; D002972:Cleft Palate / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D008297:Male; D040901:Proteomics; D012471:Salivary Proteins and Peptides / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D015854:Up-Regulation
chemical_list: D012471:Salivary Proteins and Peptides