title: SLA-DRB1 and -DQB1 genotyping by the PCR-SSOP-Luminex method.
abstract: A simple and novel genotyping method was developed to detect alleles at the swine leukocyte antigen (SLA)-DRB1 and -DQB1 class II loci by using polymerase chain reaction (PCR)-fluorescently labeled sequence-specific oligonucleotide probes (SSOPs) and Luminex 100 xMAP detection. The PCR-SSOP-Luminex method exhibited accuracy of 95% for both SLA-DRB1 and -DQB1 in 6 homozygous and 16 heterozygous pig samples as confirmed by sequencing the PCR products of the same samples. In addition, 12 low-resolution SLA class II haplotypes consisting of 7 and 9 DRB1 and DQB1 alleles were identified, respectively, in one population of 283 Landrace pigs. This genotyping method facilitates the rapid and accurate identification of two- or four-digit alleles at the SLA-DRB1 and -DQB1 loci.
authors.affiliation: Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Ando
authors.forename: A
authors.initials: A
authors.lastname: Shigenari
authors.forename: M
authors.initials: M
authors.lastname: Ota
authors.forename: M
authors.initials: M
authors.lastname: Sada
authors.forename: H
authors.initials: H
authors.lastname: Kawata
authors.forename: F
authors.initials: F
authors.lastname: Azuma
authors.forename: C
authors.initials: C
authors.lastname: Kojima-Shibata
authors.forename: M
authors.initials: M
authors.lastname: Nakajoh
authors.forename: K
authors.initials: K
authors.lastname: Suzuki
authors.forename: H
authors.initials: H
authors.lastname: Uenishi
authors.forename: J K
authors.initials: JK
authors.lastname: Kulski
authors.forename: H
authors.initials: H
authors.lastname: Inoko
mesh_terms: D000818:Animals; D005787:Gene Frequency; D056426:Genetic Loci; D005838:Genotype; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II / Q000235:genetics* / Q000276:immunology; D006650:Histocompatibility Testing / Q000379:methods / Q000662:veterinary; D015345:Oligonucleotide Probes / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods*; D013379:Substrate Specificity / Q000235:genetics; D013552:Swine / Q000235:genetics* / Q000276:immunology
chemical_list: D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D015345:Oligonucleotide Probes; C438668:swine leukocyte antigen