title: Effects of slightly acidic electrolysed drinking water on mice.
abstract: Slightly acidic electrolysed (SAE) water is a sanitizer with strong bactericidal activity due to hypochlorous acid. We assessed the safety of SAE water as drinking water for mice at a 5 ppm total residual chlorine (TRC) concentration to examine the possibility of SAE water as a labour- and energy-saving alternative to sterile water. We provided SAE water or sterile water to mice for 12 weeks, during which time we recorded changes in body weight and weekly water and food intakes. At the end of the experiment, all of the subject animals were sacrificed to assess serum aspartate aminotransferase, alanine aminotransferase and creatinine levels and to examine the main organs histopathologically under a light microscope. In addition, we investigated the bacteria levels of both types of water. We found no difference in functional and morphological health condition indices between the groups. Compared with sterile water, SAE water had a relatively higher ability to suppress bacterial growth. We suggest that SAE water at 5 ppm TRC is a safe and useful alternative to sterile water for use as drinking water in laboratory animal facilities.
authors.affiliation: Animal Research Laboratory, Bioscience Education-Research Center, Akita University, 1-1-1 Hondo, Akita 010-8543, Japan. ahin@mail.ecc.u-tokyo.ac.jp
authors.forename: Hideaki
authors.initials: H
authors.lastname: Inagaki
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Shibata
authors.forename: Takahiro
authors.initials: T
authors.lastname: Obata
authors.forename: Masami
authors.initials: M
authors.lastname: Kawagoe
authors.forename: Katsuhisa
authors.initials: K
authors.lastname: Ikeda
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Sato
authors.forename: Kazumi
authors.initials: K
authors.lastname: Toida
authors.forename: Hidemi
authors.initials: H
authors.lastname: Kushima
authors.forename: Yukihisa
authors.initials: Y
authors.lastname: Matsuda
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001835:Body Weight / Q000187:drug effects*; D003404:Creatinine / Q000097:blood; D004326:Drinking / Q000187:drug effects*; D060766:Drinking Water / Q000737:chemistry* / Q000382:microbiology; D004435:Eating / Q000187:drug effects*; D006861:Hydrogen Peroxide / Q000737:chemistry / Q000494:pharmacology*; D051379:Mice; D014781:Viscera / Q000473:pathology
chemical_list: D060766:Drinking Water; C118085:superoxidized water; D003404:Creatinine; D006861:Hydrogen Peroxide; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase