title: Crystallization and preliminary X-ray crystallographic analysis of human autotaxin.
abstract: Autotaxin (ATX), which is also known as ectonucleotide pyrophosphatase/phosphodiesterase 2 (NPP2 or ENPP2) or phosphodiesterase Iα (PD-Iα), is an extracellular lysophospholipase D which generates lysophosphatidic acid (LPA) from lysophosphatidylcholine (LPC). ATX stimulates tumour-cell migration, angiogenesis and metastasis and is an attractive target for cancer therapy. For crystallographic studies, the α isoform of human ATX was overproduced in Escherichia coli, purified and crystallized using the hanging-drop vapour-diffusion method. X-ray diffraction data were collected to 3.0 Å resolution from a monoclinic crystal form belonging to space group C2, with unit-cell parameters a = 311.4, b = 147.9, c = 176.9 Å, β = 122.6°.
authors.affiliation: School of Pharmacy, Showa University, Tokyo 142-8555, Japan.
authors.forename: Keigo
authors.initials: K
authors.lastname: Inoue
authors.forename: Nobutada
authors.initials: N
authors.lastname: Tanaka
authors.forename: Arayo
authors.initials: A
authors.lastname: Haga
authors.forename: Kyohei
authors.initials: K
authors.lastname: Yamasaki
authors.forename: Tomonobu
authors.initials: T
authors.lastname: Umeda
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Kusakabe
authors.forename: Yasumitsu
authors.initials: Y
authors.lastname: Sakamoto
authors.forename: Takamasa
authors.initials: T
authors.lastname: Nonaka
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Deyashiki
authors.forename: Kazuo T
authors.initials: KT
authors.lastname: Nakamura
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D006801:Humans; D009097:Multienzyme Complexes / Q000737:chemistry*; D043264:Phosphodiesterase I / Q000737:chemistry*; D010727:Phosphoric Diester Hydrolases; D011755:Pyrophosphatases / Q000737:chemistry*; D000099189:Lysophospholipase D
chemical_list: D009097:Multienzyme Complexes; D043264:Phosphodiesterase I; D010727:Phosphoric Diester Hydrolases; D011755:Pyrophosphatases; D000099189:Lysophospholipase D