title: Time-varying migration process of moving neutralization boundary on the immobilized pH gradient strip in the weak-base rehydration buffer.
abstract: This paper quantificationally probes into time-varying migration processes of moving neutralization boundary (MNB) on immobilized pH gradient (IPG) strip in ammonia-rehydration buffers. The time-varying migration processes are determined by both time-varying dissociation equilibria of ammonia and position-varying pH environments formed by immobilized carrier ampholytes (CAs) on the IPG strip. Thus, the local dissociation equilibria of ammonia and the position-varying pH are introduced into the recursion equation of position of MNB migrations. The theoretical position-time curves and the velocity-time curves of MNB migrations obtained by the recursion approach were satisfactorily validated by a series of images of boundary migrations from the IPG-MNB experiments by using rehydration buffers with different ammonia concentrations on pH 3-6 IPG strips. The results achieved herein have significant evidence to a quantificational understanding of the mechanism of MNB and IEF.
authors.affiliation: Separation Science Institute, Key Laboratory of Biomedical Information Engineering of Education Ministry, Xi'an Jiaotong University, Xi'an, P. R. China. lheng@mail.xjtu.edu.cn
authors.forename: Heng
authors.initials: H
authors.lastname: Liang
authors.forename: Liang-Fei
authors.initials: LF
authors.lastname: OuYang
authors.forename: Qian
authors.initials: Q
authors.lastname: Liu
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.forename: Li-Jing
authors.initials: LJ
authors.lastname: Tian
authors.forename: Yang
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000665:Ampholyte Mixtures / Q000737:chemistry*; D002021:Buffers; D007525:Isoelectric Focusing / Q000295:instrumentation*; D018892:Proton-Motive Force; D013997:Time Factors
chemical_list: D000665:Ampholyte Mixtures; D002021:Buffers