title: Simultaneous analysis of Δ(9)-tetrahydrocannabinol and 11-nor-9-carboxy-tetrahydrocannabinol in hair without different sample preparation and derivatization by gas chromatography-tandem mass spectrometry.
abstract: The present study describes a gas chromatography/tandem mass spectrometry-negative ion chemical ionization assay (GC/MS/MS-NCI) for simultaneous analysis of Δ(9)-tetrahydrocannabinol (THC) and 11-nor-9-carboxy-tetrahydrocannabinol (THCCOOH) in hair. Each hair sample, of approximately 20mg, was weighed and the sample was dissolved in 1ml of 1M sodium hydroxide (30min at 85°C) in the presence of THC-d(3) and THCCOOH-d(3). For the analysis of THC, hair samples were extracted with n-hexane:ethyl acetate (9:1) two times; acetic acid and sodium acetate buffer were added for the analysis of THCCOOH, and hair samples were re-extracted with n-hexane:ethyl acetate (9:1) two times. The extracts were then derivatized with pentafluoropropionic anhydride (PFPA) and pentafluoropropanol (PFPOH). This method allowed the analysis of THC and THCCOOH using the GC/MS/MS-NCI assay. This method was also fully validated and applied to hair specimens (n=54) collected from known cannabis users whose urine test results were positive. The concentrations of THC and THCCOOH in hair ranged from 7.52 to 60.41ng/mg and from 0.10 to 11.68pg/mg, respectively. In this paper, we simultaneously measured THC and THCCOOH in human hair using GC/MS/MS-NCI without requiring different sample preparation and derivatization procedures. The analytical sensitivity for THCCOOH in hair was good, while that for THC in hair needs to be improved in further study.
authors.affiliation: National Institute of Scientific Investigation, 331-1 Shinwol 7-Dong, Yang-Chun Gu, Seoul, South Korea.
authors.forename: Eunyoung
authors.initials: E
authors.lastname: Han
authors.forename: Yonghoon
authors.initials: Y
authors.lastname: Park
authors.forename: Eunmi
authors.initials: E
authors.lastname: Kim
authors.forename: Sangwhan
authors.initials: S
authors.lastname: In
authors.forename: Wonkyung
authors.initials: W
authors.lastname: Yang
authors.forename: Sooyeun
authors.initials: S
authors.lastname: Lee
authors.forename: Hwakyung
authors.initials: H
authors.lastname: Choi
authors.forename: Sangki
authors.initials: S
authors.lastname: Lee
authors.forename: Heesun
authors.initials: H
authors.lastname: Chung
authors.forename: Joon Myong
authors.initials: JM
authors.lastname: Song
mesh_terms: D002138:Calibration; D002188:Cannabis / Q000378:metabolism; D002623:Chemistry Techniques, Analytical / Q000379:methods; D002849:Chromatography, Gas / Q000379:methods*; D013759:Dronabinol / Q000031:analogs & derivatives* / Q000032:analysis*; D006197:Hair / Q000737:chemistry*; D006801:Humans; D007477:Ions; D008956:Models, Chemical; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013696:Temperature
chemical_list: D007477:Ions; C016780:11-nor-delta(9)-tetrahydrocannabinol-9-carboxylic acid; D013759:Dronabinol