title: Prokaryotic expression and identification of 3-1E gene of merozoite surface antigen of Eimeria acervulina.
abstract: Eimeria acervulina was isolated from chicken at Hebei province, China. The gene of merozoite surface antigen 3-1E was amplified and cloned into pET28a(+) vector and then transformed into Escherichia coli BL21 strain. Results showed that 3-1E fusion protein band of about 22 kDa was identified by SDS-PAGE. Western blot analysis indicated that the recombinant protein specifically reacted with E. acervulina polyclonal antibody.
authors.affiliation: College of Chinese Traditional Veterinary Science, Hebei Agriculture University, Dingzhou, China.
authors.forename: Yuelan
authors.initials: Y
authors.lastname: Zhao
authors.forename: Chengmin
authors.initials: C
authors.lastname: Wang
authors.forename: Yanmin
authors.initials: Y
authors.lastname: Lu
authors.forename: Said
authors.initials: S
authors.lastname: Amer
authors.forename: Ping
authors.initials: P
authors.lastname: Xu
authors.forename: Jianyong
authors.initials: J
authors.lastname: Wang
authors.forename: Junxia
authors.initials: J
authors.lastname: Lu
authors.forename: Yongzhan
authors.initials: Y
authors.lastname: Bao
authors.forename: Bolin
authors.initials: B
authors.lastname: Deng
authors.forename: Hongxuan
authors.initials: H
authors.lastname: He
authors.forename: Jianhua
authors.initials: J
authors.lastname: Qin
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000953:Antigens, Protozoan / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D000954:Antigens, Surface / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D015153:Blotting, Western; D002645:Chickens; D002681:China; D003001:Cloning, Molecular; D003048:Coccidiosis / Q000469:parasitology / Q000662:veterinary; D004539:Eimeria / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D052940:Merozoites / Q000276:immunology; D008970:Molecular Weight; D011201:Poultry Diseases / Q000469:parasitology; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D000954:Antigens, Surface; D015800:Protozoan Proteins