title: Tetraspanin CD151 is a novel prognostic marker in poor outcome endometrial cancer.
abstract: BACKGROUND
  Type II cancers account for 10% of endometrial cancers but 50% of recurrence. Response rates to chemotherapy at recurrence are poor and better prognostic markers are needed to guide therapy. CD151 is a small transmembrane protein that regulates cell migration and facilitates cancer metastasis. High CD151 expression confers poor prognosis in breast, pancreatic and colorectal cancer. The prognostic significance of tetraspanin CD151 expression in poor outcome endometrial cancers was evaluated, along with oestrogen receptor (ER), progesterone receptor (PR), p53, human epidermal growth factor receptor -2 (HER-2), and CD 151 staining compared with α6β1, α3β1 integrins, and E-cadherin.
  
  
  METHODS
  Tissue microarray constructed from 156 poor outcome endometrial cancers, tested with immunohistochemistry and staining correlated with clinicopathological data were used. A total of 131 data sets were complete for analysis.
  
  
  RESULTS
  Expression of CD151 was significantly higher in uterine papillary serous and clear cell carcinoma than in grade 3 endometrioid carcinoma, sarcoma or carcinosarcoma (P<0.001). In univariate analysis, age, stage, histology type and CD151 were significant for both recurrence free (RFS) and disease specific survival (DSS). In multivariate analyses, CD151 was significant for RFS and DSS (P=0.036 and 0.033, respectively) in triple negative (ER, PR and HER-2 negative) tumours (88/131). The HER-2, p53, ER and PR were not prognostic for survival. There was strong concordance of CD151 with E-cadherin (98%), but not with α6β1 (35%), α3β1 staining (60%).
  
  
  CONCLUSIONS
  The CD151 is a novel marker in type 2 cancers that can guide therapeutic decisions. CD151 may have an important role in tumourigenesis in some histology types.
authors.affiliation: Department of Obstetrics and Gynaecology, Cheltenham General Hospital, Gloucestershire, UK.
authors.forename: M A
authors.initials: MA
authors.lastname: Voss
authors.forename: N
authors.initials: N
authors.lastname: Gordon
authors.forename: S
authors.initials: S
authors.lastname: Maloney
authors.forename: R
authors.initials: R
authors.lastname: Ganesan
authors.forename: L
authors.initials: L
authors.lastname: Ludeman
authors.forename: K
authors.initials: K
authors.lastname: McCarthy
authors.forename: R
authors.initials: R
authors.lastname: Gornall
authors.forename: G
authors.initials: G
authors.lastname: Schaller
authors.forename: W
authors.initials: W
authors.lastname: Wei
authors.forename: F
authors.initials: F
authors.lastname: Berditchevski
authors.forename: S
authors.initials: S
authors.lastname: Sundar
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism*; D016889:Endometrial Neoplasms / Q000378:metabolism* / Q000473:pathology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D060185:Tetraspanin 24
chemical_list: D015703:Antigens, CD; D014408:Biomarkers, Tumor; C554715:CD151 protein, human; D060185:Tetraspanin 24