title: Next-generation sequencing to generate interactome datasets.
abstract: Next-generation sequencing has not been applied to protein-protein interactome network mapping so far because the association between the members of each interacting pair would not be maintained in en masse sequencing. We describe a massively parallel interactome-mapping pipeline, Stitch-seq, that combines PCR stitching with next-generation sequencing and used it to generate a new human interactome dataset. Stitch-seq is applicable to various interaction assays and should help expand interactome network mapping.
authors.affiliation: Center for Cancer Systems Biology, Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, Massachusetts, USA.
authors.forename: Haiyuan
authors.initials: H
authors.lastname: Yu
authors.forename: Leah
authors.initials: L
authors.lastname: Tardivo
authors.forename: Stanley
authors.initials: S
authors.lastname: Tam
authors.forename: Evan
authors.initials: E
authors.lastname: Weiner
authors.forename: Fana
authors.initials: F
authors.lastname: Gebreab
authors.forename: Changyu
authors.initials: C
authors.lastname: Fan
authors.forename: Nenad
authors.initials: N
authors.lastname: Svrzikapa
authors.forename: Tomoko
authors.initials: T
authors.lastname: Hirozane-Kishikawa
authors.forename: Edward
authors.initials: E
authors.lastname: Rietman
authors.forename: Xinping
authors.initials: X
authors.lastname: Yang
authors.forename: Julie
authors.initials: J
authors.lastname: Sahalie
authors.forename: Kourosh
authors.initials: K
authors.lastname: Salehi-Ashtiani
authors.forename: Tong
authors.initials: T
authors.lastname: Hao
authors.forename: Michael E
authors.initials: ME
authors.lastname: Cusick
authors.forename: David E
authors.initials: DE
authors.lastname: Hill
authors.forename: Frederick P
authors.initials: FP
authors.lastname: Roth
authors.forename: Pascal
authors.initials: P
authors.lastname: Braun
authors.forename: Marc
authors.initials: M
authors.lastname: Vidal
mesh_terms: D030562:Databases, Protein / Q000706:statistics & numerical data*; D006801:Humans; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D025941:Protein Interaction Mapping / Q000706:statistics & numerical data*; D017422:Sequence Analysis, DNA / Q000706:statistics & numerical data*; D020798:Two-Hybrid System Techniques