title: Oxidatively generated damage to DNA by UVA radiation in cells and human skin.
abstract: Exposure of cells and human skin to UVA radiation oxidatively induces damage to DNA that consists mostly of 8-oxo-7,8-dihydroguanine (8-oxoGua), together with relatively minor amounts of oxidized pyrimidine bases and oligonucleotide strand breaks. Singlet oxygen generated by the type II photosensitization mechanism forms 8-oxoGua, with a small contribution by hydroxyl radicals. The mechanisms of UVA-mediated formation of DNA oxidation products indicate the absence of induction of double-strand breaks.
authors.affiliation: Laboratoire Lésions de Acides Nucléiques, SCIB-UMR-E no 3, CEA/UJF, FRE CNRS 3200, Département de Recherche Fondamentale sur la Matière Condensée, Grenoble, France. jean.cadet@cea.fr
authors.forename: Jean
authors.initials: J
authors.lastname: Cadet
authors.forename: Thierry
authors.initials: T
authors.lastname: Douki
mesh_terms: D002478:Cells, Cultured; D053903:DNA Breaks, Double-Stranded; D004249:DNA Damage; D006147:Guanine / Q000031:analogs & derivatives / Q000378:metabolism; D006801:Humans; D017665:Hydroxyl Radical / Q000378:metabolism; D018384:Oxidative Stress; D026082:Singlet Oxygen / Q000009:adverse effects; D012867:Skin / Q000378:metabolism* / Q000528:radiation effects*; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D026082:Singlet Oxygen; D017665:Hydroxyl Radical; C024829:8-hydroxyguanine; D006147:Guanine