title: Parallel on-chip gene synthesis and application to optimization of protein expression.
abstract: Low-cost, high-throughput gene synthesis and precise control of protein expression are of critical importance to synthetic biology and biotechnology. Here we describe the development of an on-chip gene synthesis technology, which integrates on a single microchip the synthesis of DNA oligonucleotides using inkjet printing, isothermal oligonucleotide amplification and parallel gene assembly. Use of a mismatch-specific endonuclease for error correction results in an error rate of ~0.19 errors per kb. We applied this approach to synthesize pools of thousands of codon-usage variants of lacZα and 74 challenging Drosophila protein antigens, which were then screened for expression in Escherichia coli. In one round of synthesis and screening, we obtained DNA sequences that were expressed at a wide range of levels, from zero to almost 60% of the total cell protein mass. This technology may facilitate systematic investigation of the molecular mechanisms of protein translation and the design, construction and evolution of macromolecular machines, metabolic networks and synthetic cells.
authors.affiliation: Department of Biomedical Engineering, Duke University, Durham, North Carolina, USA.
authors.forename: Jiayuan
authors.initials: J
authors.lastname: Quan
authors.forename: Ishtiaq
authors.initials: I
authors.lastname: Saaem
authors.forename: Nicholas
authors.initials: N
authors.lastname: Tang
authors.forename: Siying
authors.initials: S
authors.lastname: Ma
authors.forename: Nicolas
authors.initials: N
authors.lastname: Negre
authors.forename: Hui
authors.initials: H
authors.lastname: Gong
authors.forename: Kevin P
authors.initials: KP
authors.lastname: White
authors.forename: Jingdong
authors.initials: J
authors.lastname: Tian
mesh_terms: D000465:Algorithms; D000818:Animals; D003062:Codon / Q000235:genetics; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D005813:Genes, Synthetic; D007763:Lac Operon / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015202:Protein Engineering / Q000379:methods*; D040901:Proteomics / Q000379:methods*; D017422:Sequence Analysis, DNA; D014157:Transcription Factors / Q000235:genetics
chemical_list: D003062:Codon; D029721:Drosophila Proteins; D029968:Escherichia coli Proteins; D014157:Transcription Factors