title: Instillation of mitomycin C after transurethral resection of bladder cancer impairs wound healing: an animal model.
abstract: BACKGROUND
  Mitomycin C is used in the immediate post-operative period to prevent tumor re-implantation, but it has adverse effects on the bladder. This study devised an animal model to investigate the effects of intravesical mitomycin C on wound healing.
  
  
  METHODS
  A cystotomy was made in the dome of the bladder of female rats. The mucosa of the posterior wall was scratch with closed forceps. The bladder was closed and 0.2 ml of saline with or without 0.4 mg mitomycin C was instilled into the bladder transurethrally. The rats were sacrificed 30 and 60 days after the treatment and the bladder was examined grossly and microscopically.
  
  
  RESULTS
  The most frequent histological findings in the bladder were chronic inflammation and fibrosis. Fibrosis but not chronic inflammation was significantly associated with the exposure to MMC and it persisted even 60 days after the exposure to mitomycin C.
  
  
  CONCLUSIONS
  Mitomycin C produces chronic fibrosis in rat bladder that is often seen in patients receiving prophylactic treatment with this drug.
authors.affiliation: Departments of Urology, SUNY Upstate Medical University, 750 East Adams Street, Syracuse, NY 13210, USA. houj@upstate.edu
authors.forename: Jack Cheng-Tsung
authors.initials: JC
authors.lastname: Hou
authors.forename: Steve
authors.initials: S
authors.lastname: Landas
authors.forename: Ching Y
authors.initials: CY
authors.lastname: Wang
authors.forename: Oleg
authors.initials: O
authors.lastname: Shapiro
mesh_terms: D000283:Administration, Intravesical; D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D016685:Mitomycin / Q000008:administration & dosage* / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014521:Urethra / Q000473:pathology / Q000601:surgery*; D001749:Urinary Bladder Neoplasms / Q000473:pathology / Q000601:surgery*; D013520:Urologic Surgical Procedures; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D016685:Mitomycin