title: Involvement of cancer biomarker C7orf24 in the growth of human osteosarcoma.
abstract: BACKGROUND
  Up-regulation of the expression of the gene C7orf24, encoding γ-glutamyl cyclotransferase, is a common event in cancers derived from various tissues, but its involvement in osteosarcomas (OS) has not yet been demonstrated.
  
  
  METHODS
  The expression of C7orf24 was analyzed in human OS cell lines and primary tumor samples. The biological effects of C7orf24 on growth, motility, and invasion in the OS cell lines were investigated using siRNA for C7orf24. Genes related to the function of C7orf24 were sought by genome-wide gene expression profiling.
  
  
  RESULTS
  The level of C7orf24 expression was much higher in the OS cell lines and OS primary tumors than in normal osteoblasts. Down-regulation of C7orf24 expression inhibited the growth of the cell lines in association with enhancement of cell-clustering. Treatment with C7orf24-siRNA inhibited cell motility and invasion. Gene ontology suggested the function of C7orf24 to be related to cell adhesion and protein transport.
  
  
  CONCLUSIONS
  C7orf24 is also involved in the growth of OS, and is a potential biomarker for this type of tumor.
authors.affiliation: Department of Tissue Regeneration, Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Uejima
authors.forename: Koichi
authors.initials: K
authors.lastname: Nishijo
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Kajita
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Ishibe
authors.forename: Tomoki
authors.initials: T
authors.lastname: Aoyama
authors.forename: Susumu
authors.initials: S
authors.lastname: Kageyama
authors.forename: Hideaki
authors.initials: H
authors.lastname: Iwaki
authors.forename: Takashi
authors.initials: T
authors.lastname: Nakamura
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Iida
authors.forename: Tatsuhiro
authors.initials: T
authors.lastname: Yoshiki
authors.forename: Junya
authors.initials: J
authors.lastname: Toguchida
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D015153:Blotting, Western; D001859:Bone Neoplasms / Q000235:genetics* / Q000378:metabolism; D002448:Cell Adhesion; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D002648:Child; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007124:Immunoenzyme Techniques; D020411:Oligonucleotide Array Sequence Analysis; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism; D012516:Osteosarcoma / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D005722:gamma-Glutamylcyclotransferase / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; D012333:RNA, Messenger; D005722:gamma-Glutamylcyclotransferase