title: Fatty acid composition of common barbel (Barbus barbus) roe and evaluation of its haemolytic and cytotoxic activities.
abstract: Eggs of the common barbel (Barbus barbus) cause intoxication in humans after ingestion. In this work, the chemical composition of the haemolytically active fraction from methanolic barbel roe extract was analyzed. Compounds showing haemolytic activity and cytotoxicity towards normal and transformed cell lines were isolated and identified as polyunsaturated fatty acids, using online liquid chromatography-electrospray ionization mass spectrometry through tandem fragmentation experiments (HPLC-MS/MS). Arachidonic acid (C20:4), docosahexaenoic acid (C22:6) and eicosapentaenoic acid (C20:5) proved to be the three most abundant members of a complex series of free fatty acids ranging from C14:0 to C24:5.
authors.affiliation: Laboratorio di Chimica Bioorganica, Dipartimento di Fisica, Università di Trento, via Sommarive 14, I-38123 Povo Trento, Italy.
authors.forename: Ines
authors.initials: I
authors.lastname: Mancini
authors.forename: Andrea
authors.initials: A
authors.lastname: Defant
authors.forename: Tina
authors.initials: T
authors.lastname: Mesarič
authors.forename: Franc
authors.initials: F
authors.lastname: Potočnik
authors.forename: Urška
authors.initials: U
authors.lastname: Batista
authors.forename: Graziano
authors.initials: G
authors.lastname: Guella
authors.forename: Tom
authors.initials: T
authors.lastname: Turk
authors.forename: Kristina
authors.initials: K
authors.lastname: Sepčić
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D002461:Cell Line, Transformed; D002851:Chromatography, High Pressure Liquid; D003530:Cyprinidae / Q000378:metabolism*; D004281:Docosahexaenoic Acids / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D015118:Eicosapentaenoic Acid / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D005069:Evaluation Studies as Topic; D005231:Fatty Acids, Unsaturated / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D005260:Female; D006461:Hemolysis; D006801:Humans; D000432:Methanol / Q000737:chemistry; D010063:Ovum / Q000737:chemistry / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization; D018675:Toxicity Tests
chemical_list: D005231:Fatty Acids, Unsaturated; D004281:Docosahexaenoic Acids; D016718:Arachidonic Acid; D015118:Eicosapentaenoic Acid; D000432:Methanol