title: [Expression and significance of interleukin-23 and its related cytokines in chronic idiopathic thrombocytopenic purpura].
abstract: The aim of this study was to investigate the expression and immunologic regulation function of interleukin-23 and its related cytokines in chronic idiopathic thrombocytopenic purpura (ITP) patients. Levels of cytokines in peripheral blood mononuclear cells (PBMNC) were detected by reverse-transcription real-time polymerase chain reaction in 30 patients with chronic ITP and 15 healthy volunteers. The quantity of IL-23, IL-12, IL-17 in serum was detected by enzyme-linked immunosorbent assay (ELISA). The results showed that low detectable mRNA levels of IL-23p19, IL-12p35, IL-27 and IL-12p40 were found in all patients and healthy persons. Trace of IL-17 mRNA were expressed in PBMNC of part of patients and normal controls. Levels of IL-12p35, IL-27, IL-17 mRNA between healthy persons and chronic ITP patients were not statistically different. Compared with normal controls, patients showed the lower expression levels of IL-23p19 and IL-12p40 mRNA (p < 0.01). The IL-12 levels of chronic ITP patients were significantly higher than that of normal controls (p < 0.01). The IL-23 and IL-17 levels of chronic ITP patients were same to that of normal controls. It is concluded that the imbalance of T cell subsets in ITP patients mainly associated with IL-12, but not with IL-23 and IL-17.
authors.affiliation: Department of Hematology, Kunming Medicine College, Kunming, Yunan Province, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
authors.forename: Yong-Zhi
authors.initials: YZ
authors.lastname: Li
authors.forename: Cai-Xia
authors.initials: CX
authors.lastname: Wei
authors.forename: Cheng-Ping
authors.initials: CP
authors.lastname: Li
authors.forename: Wei-Jia
authors.initials: WJ
authors.lastname: Li
authors.forename: Hong
authors.initials: H
authors.lastname: Yang
mesh_terms: D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D002908:Chronic Disease; D005260:Female; D006801:Humans; D018664:Interleukin-12 / Q000378:metabolism*; D053765:Interleukin-12 Subunit p35 / Q000378:metabolism; D053762:Interleukin-12 Subunit p40 / Q000378:metabolism; D020381:Interleukin-17 / Q000378:metabolism; D053759:Interleukin-23 / Q000378:metabolism*; D053760:Interleukin-23 Subunit p19 / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000378:metabolism*; D055815:Young Adult
chemical_list: D053765:Interleukin-12 Subunit p35; D053762:Interleukin-12 Subunit p40; D020381:Interleukin-17; D053759:Interleukin-23; D053760:Interleukin-23 Subunit p19; D018664:Interleukin-12