title: Two-phase temporary immersion system for Agrobacterium rhizogenes genetic transformation of sage (Salvia tomentosa Mill.).
abstract: Hairy root cultures of Salvia tomentosa were initiated by transformation with Agrobacterium rhizogenes. To prevent necrosis in the explants and to protect young hairy roots, Amberlite XAD-4 resin, in combination with a temporary immersion cultivation system, was applied. HPLC analyzes showed that the resin adsorbed more than 93% of the released phenolic acids and 100% of the released flavonoids. The decreased content of the released phenolics significantly reduced their destructive effects on the plant tissues, prevented, and speeded up the appearance of hairy roots.
authors.affiliation: Laboratory of Applied Biotechnologies, The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 139 Ruski Blvd, 4000 Plovdiv, Bulgaria.
authors.forename: Andrey
authors.initials: A
authors.lastname: Marchev
authors.forename: Vasil
authors.initials: V
authors.lastname: Georgiev
authors.forename: Ivan
authors.initials: I
authors.lastname: Ivanov
authors.forename: Ilian
authors.initials: I
authors.lastname: Badjakov
authors.forename: Atanas
authors.initials: A
authors.lastname: Pavlov
mesh_terms: D000042:Absorption; D060054:Agrobacterium / Q000235:genetics*; D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000378:metabolism; D018014:Gene Transfer Techniques; D007101:Immersion; D007475:Ion Exchange Resins; D010636:Phenols / Q000378:metabolism; D027544:Salvia / Q000235:genetics*; D014170:Transformation, Genetic
chemical_list: D005419:Flavonoids; D007475:Ion Exchange Resins; D010636:Phenols