title: Storage of cell lines.
abstract: The successful storage of cell lines depends upon many factors, including the condition of the cells to be frozen and the experience of the operator. Attempting to freeze down unhealthy, contaminated or poorly labelled cells can have huge implications for a research laboratory. This chapter outlines the importance of good record keeping, vigilant monitoring, aseptic technique, and high-quality reagents in the successful storage and downstream propagation of cell lines.
authors.affiliation: Translational Oncology Research Centre, Queen Alexandra Hospital, Portsmouth, UK. iancreephd@gmail.com
authors.forename: Katharine A
authors.initials: KA
authors.lastname: Parker
mesh_terms: D002021:Buffers; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D015925:Cryopreservation; D005990:Glycerol / Q000737:chemistry; D007202:Indicators and Reagents / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry; D013048:Specimen Handling / Q000379:methods*
chemical_list: D002021:Buffers; D007202:Indicators and Reagents; D011137:Polystyrenes; D005990:Glycerol