title: Expression of urocortin and corticotropin-releasing hormone receptors in the bovine adrenal gland.
abstract: Urocortin (UCN), a 40 amino acid peptide, is a corticotrophin-releasing hormone (CRH)-related peptide. The biological actions of CRH family peptides are mediated via two types of G protein-coupled receptors, CRH type 1 receptor (CRHR1) and CRH type 2 receptor (CRHR2). The aim of the present study was to investigate the expression of UCN, CRHR1 and CRHR2 by immunohistochemistry, western blot and real-time RT-PCR in the bovine adrenal gland to clarify the mechanisms of the intra-adrenal CRH-based regulatory system. The results showed that UCN, CRHR1 and CRHR2 were expressed in both the cortex and medulla. Specifically, UCN-immunoreactivity (IR) was distributed in the outer part of the zona fasciculata and in the zona reticularis of the cortex and in the medulla. UCN and CRHR2 mRNA expression levels were higher in the cortex than in the medulla, while CRHR1 mRNA levels were undetectable in the cortex. These results suggest that UCN, CRHR1 and CRHR2 are expressed in the bovine adrenal gland and that UCN might play a role in the intra-adrenal CRH-based regulatory system through an autocrine mechanism.
authors.affiliation: Department of Structure, Functions and Biological Technologies, University of Naples Federico II, Naples, Italy. caterina.squillacioti@unina.it
authors.forename: Caterina
authors.initials: C
authors.lastname: Squillacioti
authors.forename: Adriana
authors.initials: A
authors.lastname: De Luca
authors.forename: Giovanna
authors.initials: G
authors.lastname: Liguori
authors.forename: Salvatore
authors.initials: S
authors.lastname: Paino
authors.forename: Nicola
authors.initials: N
authors.lastname: Mirabella
mesh_terms: D000311:Adrenal Glands / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D002417:Cattle; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D018019:Receptors, Corticotropin-Releasing Hormone / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D054832:Urocortins / Q000378:metabolism*
chemical_list: D018019:Receptors, Corticotropin-Releasing Hormone; D054832:Urocortins