title: [Identification of known microRNAs in root and leaf of maize by deep sequencing].
abstract: microRNA (miRNAs) is a newly identified class of 20-24 nt non-protein-coding and endogenous small RNA, which plays an important role in plant growth, development and response to environmental stresses. Combined with bioinformatic method, the types, abundance, and targets of known miRNAs in root and leaf of maize (Zea mays L.) were analyzed by small RNA deep sequencing technology, which was based on Illumina/Solexa principium. The results indicated that 92 known miRNAs were detected in maize root, which were attributed to 18 miRNA families and their abundance ranged from 1 to 105,943 reads. Synchronously, 86 known miRNAs were detected in maize leaf, which were attributed to 17 miRNA families and their abundance ranged from 1 to 85,973 reads. The target gene prediction showed that 54 putative target genes as these known miRNAs were predicted. Some of them were involved in the following processes, such as transcription regulation, substance and energy metabolism, electron transport, stress response, and signal transduction through further function prediction. In conclusion, there were obvious differences in both types and abundance of known miRNAs between root and leaf in maize.
authors.affiliation: Maize Research Institute, Sichuan Agricultural University, Ya 'an 625014, China. kathy.chen@yeah.net
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.forename: Hai-Jian
authors.initials: HJ
authors.lastname: Lin
authors.forename: Guang-Tang
authors.initials: GT
authors.lastname: Pan
authors.forename: Zhi-Ming
authors.initials: ZM
authors.lastname: Zhang
authors.forename: Biao
authors.initials: B
authors.lastname: Zhang
authors.forename: Ya-ou
authors.initials: YO
authors.lastname: Shen
authors.forename: Cheng
authors.initials: C
authors.lastname: Qin
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.forename: Mao-Jun
authors.initials: MJ
authors.lastname: Zhao
mesh_terms: D001483:Base Sequence; D018506:Gene Expression Regulation, Plant; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D018515:Plant Leaves / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA / Q000379:methods*; D003313:Zea mays / Q000235:genetics* / Q000378:metabolism
chemical_list: D035683:MicroRNAs