title: Influence of ammonium sulphate feeding time on fed-batch Arthrospira (Spirulina) platensis cultivation and biomass composition with and without pH control.
abstract: Previous work demonstrated that a mixture of NH(4)Cl and KNO(3) as nitrogen source was beneficial to fed-batch Arthrospira (Spirulina) platensis cultivation, in terms of either lower costs or higher cell concentration. On the basis of those results, this study focused on the use of a cheaper nitrogen source mixture, namely (NH(4))(2)SO(4) plus NaNO(3), varying the ammonium feeding time (T=7-15 days), either controlling the pH by CO(2) addition or not. A. platensis was cultivated in mini-tanks at 30°C, 156 μmol photons m(-2) s(-1), and starting cell concentration of 400 mg L(-1), on a modified Schlösser medium. T=13 days under pH control were selected as optimum conditions, ensuring the best results in terms of biomass production (maximum cell concentration of 2911 mg L(-1), cell productivity of 179 mg L(-1)d(-1) and specific growth rate of 0.77 d(-1)) and satisfactory protein and lipid contents (around 30% each).
authors.affiliation: Department of Biochemical and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 580, B-16, 05508-900 São Paulo - SP, Brazil.
authors.forename: Mayla Santos
authors.initials: MS
authors.lastname: Rodrigues
authors.forename: Lívia Seno
authors.initials: LS
authors.lastname: Ferreira
authors.forename: Attilio
authors.initials: A
authors.lastname: Converti
authors.forename: Sunao
authors.initials: S
authors.lastname: Sato
authors.forename: João Carlos Monteiro
authors.initials: JC
authors.lastname: de Carvalho
mesh_terms: D000645:Ammonium Sulfate / Q000494:pharmacology*; D018533:Biomass; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D053545:Spirulina / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development*; D013997:Time Factors
chemical_list: D000645:Ammonium Sulfate