title: Association of the angiotensinogen M235T and APO E gene polymorphisms in Turkish type 2 diabetic patients with and without nephropathy.
abstract: BACKGROUND
  Diabetic nephropathy (DN) is a leading cause of diabetes-related morbidity and mortality. The aim of this study was to evaluate the relationship of AGT M235T and apoprotein E (APO E) gene polymorphism with DN in Turkish patients of Type 2 diabetes, and to compare genotype and allele distributions among DN patients, non-DN patients, and healthy controls.
  
  
  METHODS
  AGT M235T and APO E genotype and allele analysis were performed in 111 DN patients, 108 non-DN patients, 106 healthy control subjects for APO E genotype, and 100 for AGT M235T genotype polymorphism. APO E and AGT M235T genotype were determined by RFLP-PCR.
  
  
  RESULTS
  The frequencies of APO E ε2/3, ε 3/3, ε 3/4 genotypes were 22.7%, 60%, 60%, respectively, among DN patients and 6.6%, 80%, 10.4%, respectively (p < 0.001), in the non-DN patients. The frequencies of AGT M235T MM, MT, TT genotypes among the same groups were 17%, 46%, 37% and 21%, 63%, 16%, respectively (p < 0.02). Having the ε2/3 genotype and TT genotype increased the risk for DN nephropathy [4.8-fold (95% CI: 1.94-11.67), 2.9-fold (95% CI: 1.27-6.69), respectively].
  
  
  CONCLUSIONS
  Our study has shown that AGT M235T TT genotype and APO E ε 2/3 genotype may be linked to a risk for DN among Turkish population.
authors.affiliation: Department of Nephrology, Faculty of Medicine, Gazi University, Ankara, Turkey.
authors.forename: Kadriye Altok
authors.initials: KA
authors.lastname: Reis
authors.forename: Fatma Ayerden
authors.initials: FA
authors.lastname: Ebinç
authors.forename: Eyüp
authors.initials: E
authors.lastname: Koç
authors.forename: Hüseyin
authors.initials: H
authors.lastname: Demirci
authors.forename: Yasemin
authors.initials: Y
authors.lastname: Erten
authors.forename: Galip
authors.initials: G
authors.lastname: Güz
authors.forename: Ulver Boztepe
authors.initials: UB
authors.lastname: Derici
authors.forename: Musa
authors.initials: M
authors.lastname: Bali
authors.forename: Oğuz
authors.initials: O
authors.lastname: Söylemezoğlu
authors.forename: Turgay
authors.initials: T
authors.lastname: Arınsoy
authors.forename: Sükrü
authors.initials: S
authors.lastname: Sindel
mesh_terms: D000368:Aged; D000483:Alleles; D019943:Amino Acid Substitution; D000808:Angiotensinogen / Q000235:genetics*; D001057:Apolipoproteins E / Q000235:genetics*; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000150:complications* / Q000235:genetics; D003928:Diabetic Nephropathies / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D014421:Turkey
chemical_list: D001057:Apolipoproteins E; D000808:Angiotensinogen