title: Electrospun chitosan-graft-poly (ɛ-caprolactone)/poly (ɛ-caprolactone) nanofibrous scaffolds for retinal tissue engineering.
abstract: A promising therapy for retinal diseases is to employ biodegradable scaffolds to deliver retinal progenitor cells (RPCs) for repairing damaged or diseased retinal tissue. In the present study, cationic chitosan-graft-poly(ɛ-caprolactone)/polycaprolactone (CS-PCL/PCL) hybrid scaffolds were successfully prepared by electrospinning. Characterization of the obtained nanofibrous scaffolds indicated that zeta-potential, fiber diameter, and the content of amino groups on their surface were closely correlated with the amount of CS-PCL in CS-PCL/PCL scaffolds. To assess the cell-scaffold interaction, mice RPCs (mRPCs) were cultured on the electrospun scaffolds for 7 days. In-vitro proliferation assays revealed that mRPCs proliferated faster on the CS-PCL/PCL (20/80) scaffolds than the other electrospun scaffolds. Scanning electron microscopy and the real-time quantitative polymerase chain reaction results showed that mRPCs grown on CS-PCL/PCL (20/80) scaffolds were more likely to differentiate towards retinal neurons than those on PCL scaffolds. Taken together, these results suggest that CS-PCL/PCL(20/80) scaffolds have potential application in retinal tissue engineering.
authors.affiliation: Department of Ophthalmology, Shanghai Ninth People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Honglin
authors.initials: H
authors.lastname: Chen
authors.forename: Xianqun
authors.initials: X
authors.lastname: Fan
authors.forename: Jing
authors.initials: J
authors.lastname: Xia
authors.forename: Ping
authors.initials: P
authors.lastname: Chen
authors.forename: Xiaojian
authors.initials: X
authors.lastname: Zhou
authors.forename: Jin
authors.initials: J
authors.lastname: Huang
authors.forename: Jiahui
authors.initials: J
authors.lastname: Yu
authors.forename: Ping
authors.initials: P
authors.lastname: Gu
mesh_terms: D000818:Animals; D001483:Base Sequence; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry; D049109:Cell Proliferation; D002478:Cells, Cultured; D048271:Chitosan / Q000138:chemical synthesis / Q000737:chemistry; D017931:DNA Primers / Q000235:genetics; D015870:Gene Expression; D008422:Materials Testing; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers / Q000737:chemistry*; D050997:Nanomedicine; D011091:Polyesters / Q000138:chemical synthesis / Q000737:chemistry; D012160:Retina / Q000166:cytology / Q000378:metabolism; D012164:Retinal Diseases / Q000628:therapy*; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D017931:DNA Primers; D011091:Polyesters; C016240:polycaprolactone; D048271:Chitosan