title: Zinc binding to human lactogenic hormones and the human prolactin receptor.
abstract: Zinc half sites are present in all human lactogenic hormones: human prolactin (hPRL), growth hormone (hGH), placental lactogens (hPL) and the hPRL receptor (hPRLr). The influence of divalent zinc (Zn(2+)) as measured by intrinsic fluorescence or FRET in each of these hormones is unique and is affected by the presence of varying stoichiometries of hPRLr. These data show that both Zn(2+) and hPRLr binding influence hPRL conformers in an interdependent fashion. Although each of these three lactogenic hormones bind hPRLr and induce a biological response that is sensitive to the presence of increasing concentrations of Zn(2+), each hormone is unique in the mechanistic details of this process.
authors.affiliation: Ohio State Biochemistry Program, The Ohio State University, Columbus, OH 43210, United States. Voorhees.6@osu.edu
authors.forename: Jeffrey L
authors.initials: JL
authors.lastname: Voorhees
authors.forename: Geeta Vittal
authors.initials: GV
authors.lastname: Rao
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Gordon
authors.forename: Charles L
authors.initials: CL
authors.lastname: Brooks
mesh_terms: D001665:Binding Sites; D031541:Fluorescence Resonance Energy Transfer; D019382:Human Growth Hormone / Q000378:metabolism; D006801:Humans; D010907:Pituitary Hormones / Q000378:metabolism*; D010928:Placental Lactogen / Q000378:metabolism; D011388:Prolactin / Q000378:metabolism; D011487:Protein Conformation; D011981:Receptors, Prolactin / Q000378:metabolism*; D015032:Zinc / Q000378:metabolism*
chemical_list: D010907:Pituitary Hormones; D011981:Receptors, Prolactin; D019382:Human Growth Hormone; D011388:Prolactin; D010928:Placental Lactogen; D015032:Zinc