title: Protective effect of ε-viniferin on β-amyloid peptide aggregation investigated by electrospray ionization mass spectrometry.
abstract: Abnormal β-amyloid peptide accumulation and aggregation is considered to be responsible for the formation and cerebral deposition of senile plaques in the brains of patients with Alzheimer's disease (AD). Inhibition of the formation of β-amyloid (Aβ) fibrils would be an attractive therapeutic target for the treatment of AD. Resveratrol and its derivatives exhibit a broad range of pharmacological properties such as protection against cardiovascular diseases and cancers, as well as promoting antiaging effects. We reported previously that ε-viniferin glucoside (VG), a resveratrol-derived dimer, strongly inhibits Aβ (25-35) fibril formation in vitro. In this study, we investigated the effects of VG on the aggregation of the full-length peptides (Aβ (1-40) and Aβ (1-42)) and on the β-amyloid-induced toxicity in PC12 cells. VG inhibited Aβ cytotoxicity and the non-covalent complex between VG and Aβ was observed by electrospray ionization mass spectrometry.
authors.affiliation: GESVAB, Université de Bordeaux, Villenave d'Ornon, France. tristan.richard@u-bordeaux2.fr
authors.forename: Tristan
authors.initials: T
authors.lastname: Richard
authors.forename: Pascal
authors.initials: P
authors.lastname: Poupard
authors.forename: Merian
authors.initials: M
authors.lastname: Nassra
authors.forename: Yorgos
authors.initials: Y
authors.lastname: Papastamoulis
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Iglésias
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Krisa
authors.forename: Pierre
authors.initials: P
authors.lastname: Waffo-Teguo
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Mérillon
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Monti
mesh_terms: D000544:Alzheimer Disease / Q000188:drug therapy / Q000378:metabolism; D016229:Amyloid beta-Peptides / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D000818:Animals; D001572:Benzofurans / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D016716:PC12 Cells; D051381:Rats; D021241:Spectrometry, Mass, Electrospray Ionization; D013267:Stilbenes / Q000302:isolation & purification / Q000494:pharmacology*; D027843:Vitis / Q000737:chemistry*
chemical_list: D016229:Amyloid beta-Peptides; D001572:Benzofurans; D013267:Stilbenes; C091891:epsilon-viniferin