title: In vitro sealing potential of a self-adhesive pit and fissure sealant.
abstract: OBJECTIVE
  To study the sealing ability of a self-adhesive sealant.
  
  
  METHODS
  Thirty-five extracted molars were assigned to seven groups: group 1, 35% phosphoric acid (PA) followed by Clinpro Sealant (CL) as the control group; group 2, Enamel Loc (EL), a self-adhesive sealant; group 3, PA followed by EL; group 4, Adper Prompt L-Pop (AP) followed by CL; group 5, AP followed by EL; group 6, PA followed by Adper Single Bond Plus (SB) and CL; and group 7, PA followed by SB and EL. Specimens were thermocycled and immersed in 50 wt% ammoniac silver nitrate. Silver leakage was measured in ranks from 0 to 4 and analyzed with Kruskal-Wallis and Mann-Whitney tests at P < .05. One additional tooth per group was used to study the enamel etching pattern. Specimens were observed under a field-emission SEM.
  
  
  RESULTS
  The least silver infiltration was measured for group 1 (control group), which was significantly different from all other six groups. Etching enamel prior to the application of EL resulted in significantly less leakage than all other groups, except for the control group. EL alone resulted in similar leakage to EL with either AP or SB. None of the groups resulted in perfectly sealed interfaces. Under SEM, EL was unable to etch enamel. The self-etch adhesive AP resulted in a less defined etching pattern than the etch-and-rinse adhesive SB.
  
  
  CONCLUSIONS
  Etching enamel with phosphoric acid is still essential for sealing the enamel-sealant interface. The self-adhesive sealant tested in this study may not be indicated for clinical use.
authors.affiliation: Division of Operative Dentistry, Department of Restorative Sciences, University of Minnesota, Minneapolis, MN 55455, USA. perdi001@umn.edu
authors.forename: Jorge
authors.initials: J
authors.lastname: Perdigão
authors.forename: Ana
authors.initials: A
authors.lastname: Sezinando
authors.forename: George
authors.initials: G
authors.lastname: Gomes
mesh_terms: D000134:Acid Etching, Dental / Q000379:methods; D017438:Bisphenol A-Glycidyl Methacrylate / Q000737:chemistry; D003188:Composite Resins / Q000737:chemistry; D003743:Dental Enamel / Q000648:ultrastructure; D003763:Dental Leakage / Q000145:classification; D006801:Humans; D007091:Image Processing, Computer-Assisted; D055115:Light-Curing of Dental Adhesives; D008422:Materials Testing; D008689:Methacrylates / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D063065:Organophosphonates / Q000737:chemistry; D010895:Pit and Fissure Sealants / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D011109:Polymethacrylic Acids / Q000737:chemistry; D011140:Polyurethanes / Q000737:chemistry; D019279:Resin Cements / Q000737:chemistry; D012834:Silver; D016622:Silver Staining; D013499:Surface Properties; D013696:Temperature; D013997:Time Factors; D014233:Tricarboxylic Acids / Q000737:chemistry
chemical_list: C500418:Clinpro Sealant; D003188:Composite Resins; D008689:Methacrylates; D063065:Organophosphonates; D010895:Pit and Fissure Sealants; D011109:Polymethacrylic Acids; D011140:Polyurethanes; C408584:Prompt L-Pop; D019279:Resin Cements; C454540:Scotchbond etchant; C511238:Single Bond Plus; D014233:Tricarboxylic Acids; C000212:ammoniacal silver; C029824:urethane dimethacrylate luting resin; C020946:triethylene glycol dimethacrylate; D012834:Silver; D011092:Polyethylene Glycols; D017438:Bisphenol A-Glycidyl Methacrylate; C098756:4-methacryloxyethyltrimellitic acid