title: Protective role of cytosolic NADP(+)-dependent isocitrate dehydrogenase, IDH1, in ischemic pre-conditioned kidney in mice.
abstract: Ischemic pre-conditioning protects the kidney against subsequent ischemia/reperfusion (I/R). This study investigated the role of cytosolic NADP(+)-dependent isocitrate dehydrogenase (IDH1), a producer of NADPH, in the ischemic pre-conditioning. Mice were pre-conditioned by 30 min of renal ischemia and 8 days of reperfusion. In non-pre-conditioned mice 30 min of ischemia had significantly increased the levels of plasma creatinine, BUN, lipid peroxidation and hydrogen peroxide in kidneys, whereas in pre-conditioned mice, the ischemia did not increase them. The reductions of reduced glutathione and NADPH after I/R were greater in non-pre-conditioned mice than in pre-conditioned mice. Ischemic pre-conditioning prevented the I/R-induced decreases in IDH1 activity and expression, but not in glucose-6-phosphate dehydrogenase activity. In conclusion, protection of the kidney afforded by ischemic pre-conditioning may be associated with increased activity of IDH1 which relates to increased levels of NADPH, increased ratios of GSH/total glutathione, less oxidative stress and less kidney injury induced by subsequent I/R insult.
authors.affiliation: Department of Anatomy and BK 21 Project, Kyungpook National University School of Medicine, Daegu, 700-422, Republic of Korea.
authors.forename: Jinu
authors.initials: J
authors.lastname: Kim
authors.forename: Jee In
authors.initials: JI
authors.lastname: Kim
authors.forename: Hee-Seong
authors.initials: HS
authors.lastname: Jang
authors.forename: Jeen-Woo
authors.initials: JW
authors.lastname: Park
authors.forename: Kwon Moo
authors.initials: KM
authors.lastname: Park
mesh_terms: D000818:Animals; D001806:Blood Urea Nitrogen; D003404:Creatinine / Q000097:blood; D003600:Cytosol / Q000201:enzymology; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D005978:Glutathione / Q000097:blood; D006861:Hydrogen Peroxide / Q000097:blood; D007511:Ischemia / Q000201:enzymology; D019194:Ischemic Preconditioning; D007521:Isocitrate Dehydrogenase / Q000378:metabolism*; D007668:Kidney / Q000098:blood supply / Q000201:enzymology*; D015227:Lipid Peroxidation; D051379:Mice; D008810:Mice, Inbred C57BL; D009249:NADP / Q000378:metabolism; D018384:Oxidative Stress; D015427:Reperfusion Injury
chemical_list: D009249:NADP; D003404:Creatinine; D006861:Hydrogen Peroxide; D007521:Isocitrate Dehydrogenase; C543588:IDH1 protein, human; D005954:Glucosephosphate Dehydrogenase; D005978:Glutathione