title: Development of a PIXE analysis method for the determination of the biopersistence of SiC and TiC nanoparticles in rat lungs.
abstract: With the advent of nanoparticles produced in high quantities and employed in products or processes, the need to evaluate their potential toxicological effects is necessary. For this purpose, biopersistence studies are needed to assess the possible effects of nanoparticles in parallel with a proper characterization. The insoluble character of many nanomaterials makes traditional chemical analytical methods unapplicable for the ex-vivo measurements of their concentration in organs. Ion beam-based techniques such as Particle-Induced X-ray Emission (PIXE) can solve this difficulty. We illustrate that by the measurement of biopersistence of SiC and TiC nanoparticles instilled in rats lungs and investigated over a 60-day time span. The results can be obtained within minutes and the limits of detection are within ppm levels.
authors.affiliation: Research Centre for the Physics of Matter and Radiation (PMR-LARN - NARILIS), University of Namur, Namur, Belgium. omar.lozanogarcia@fundp.ac.be
authors.forename: Omar
authors.initials: O
authors.lastname: Lozano
authors.forename: Jorge
authors.initials: J
authors.lastname: Mejia
authors.forename: Bernard
authors.initials: B
authors.lastname: Masereel
authors.forename: Olivier
authors.initials: O
authors.lastname: Toussaint
authors.forename: Dominique
authors.initials: D
authors.lastname: Lison
authors.forename: Stéphane
authors.initials: S
authors.lastname: Lucas
mesh_terms: D000818:Animals; D017554:Carbon Compounds, Inorganic / Q000008:administration & dosage / Q000032:analysis* / Q000493:pharmacokinetics; D004867:Equipment Design; D005260:Female; D007322:Instillation, Drug; D008168:Lung / Q000378:metabolism*; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000008:administration & dosage / Q000032:analysis* / Q000648:ultrastructure; D010316:Particle Size; D051381:Rats; D017208:Rats, Wistar; D017655:Silicon Compounds / Q000008:administration & dosage / Q000032:analysis* / Q000493:pharmacokinetics; D012995:Solubility; D013052:Spectrometry, X-Ray Emission / Q000379:methods*; D013499:Surface Properties; D014018:Tissue Distribution; D014025:Titanium / Q000008:administration & dosage / Q000032:analysis* / Q000493:pharmacokinetics
chemical_list: D017554:Carbon Compounds, Inorganic; D017655:Silicon Compounds; C096521:titanium carbide; D014025:Titanium; C022088:silicon carbide