title: Determination of valganciclovir and ganciclovir in human plasma by liquid chromatography tandem mass spectrometric detection.
abstract: OBJECTIVE
  The aim of this work was to develop a simple, sensitive and selective LC/MS/MS method for the assay of valganciclovir and ganciclovir in human plasma.
  
  
  METHODS
  Sample preparation involved solid phase extraction on mix mode cation exchanger. Separation was performed on Chromolith RP18e column using water, trifluoroacetic acid (1M, pH 4.4) and methanol (29.9:0.1:70, v/v) as mobile phase. Both analytes were detected by electro spray ionization mass spectrometry in positive ion multiple reaction monitoring mode.
  
  
  RESULTS
  CCs with good linearties having r≥0.9990 and ≥0.9992 were obtained in the range of 5-800ng/mL and 70-11,200ng/mL for valganciclovir and ganciclovir, respectively. The extraction recoveries were around 85% for both the analytes.
  
  
  CONCLUSIONS
  The method provided a simple and selective procedure that can be easily used for the evaluation of the pharmacokinetic profile of valganciclovir and ganciclovir in human plasma.
authors.affiliation: Clinical Pharmacology and Pharmacokinetics, Ranbaxy Research Laboratories, Plot GP-5, Sector 18, HSIDC, Gurgaon, Haryana-122015, India. onkar.singh@ranbaxy.com
authors.forename: Onkar
authors.initials: O
authors.lastname: Singh
authors.forename: Saurabh
authors.initials: S
authors.lastname: Saxena
authors.forename: Sanjeev
authors.initials: S
authors.lastname: Mishra
authors.forename: Arshad
authors.initials: A
authors.lastname: Khuroo
authors.forename: Tausif
authors.initials: T
authors.lastname: Monif
mesh_terms: D000212:Acyclovir / Q000031:analogs & derivatives / Q000097:blood / Q000737:chemistry; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D004355:Drug Stability; D015774:Ganciclovir / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics; D006801:Humans; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013997:Time Factors; D000077483:Valacyclovir; D000077562:Valganciclovir; D014633:Valine / Q000031:analogs & derivatives / Q000097:blood / Q000737:chemistry
chemical_list: D000077562:Valganciclovir; D014633:Valine; D000077483:Valacyclovir; D015774:Ganciclovir; D000212:Acyclovir