title: Regulatory response to carbon starvation in Caulobacter crescentus.
abstract: Bacteria adapt to shifts from rapid to slow growth, and have developed strategies for long-term survival during prolonged starvation and stress conditions. We report the regulatory response of C. crescentus to carbon starvation, based on combined high-throughput proteome and transcriptome analyses. Our results identify cell cycle changes in gene expression in response to carbon starvation that involve the prominent role of the FixK FNR/CAP family transcription factor and the CtrA cell cycle regulator. Notably, the SigT ECF sigma factor mediates the carbon starvation-induced degradation of CtrA, while activating a core set of general starvation-stress genes that respond to carbon starvation, osmotic stress, and exposure to heavy metals. Comparison of the response of swarmer cells and stalked cells to carbon starvation revealed four groups of genes that exhibit different expression profiles. Also, cell pole morphogenesis and initiation of chromosome replication normally occurring at the swarmer-to-stalked cell transition are uncoupled in carbon-starved cells.
authors.affiliation: Department of Developmental Biology, Stanford University School of Medicine, Stanford, California, United States of America.
authors.forename: Leticia
authors.initials: L
authors.lastname: Britos
authors.forename: Eduardo
authors.initials: E
authors.lastname: Abeliuk
authors.forename: Thomas
authors.initials: T
authors.lastname: Taverner
authors.forename: Mary
authors.initials: M
authors.lastname: Lipton
authors.forename: Harley
authors.initials: H
authors.lastname: McAdams
authors.forename: Lucy
authors.initials: L
authors.lastname: Shapiro
mesh_terms: D000222:Adaptation, Physiological / Q000187:drug effects / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002244:Carbon / Q000172:deficiency* / Q000494:pharmacology; D016935:Caulobacter crescentus / Q000166:cytology / Q000187:drug effects / Q000235:genetics*; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D053263:Gene Regulatory Networks / Q000235:genetics; D009068:Movement / Q000187:drug effects; D020543:Proteome / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018085:Regulon / Q000235:genetics; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D020543:Proteome; D012333:RNA, Messenger; D014157:Transcription Factors; D002244:Carbon