title: Role of Carum copticum seeds in modulating chromium-induced toxicity on human bronchial epithelial cells and human peripheral blood lymphocytes.
abstract: Carum copticum seeds are well known for ailment of various diseases since ancient times. The present study pertains to investigate modulatory effects of methanolic extract of C. copticum seeds (MCE) against hexavalent chromium induced cytotoxicity, genotoxicity, apoptosis and oxidative stress on human bronchial epithelial cells (BEAS-2B) and isolated human peripheral blood lymphocyte (PBL) in vitro. Treatment of BEAS-2B and PBL with MCE prior to potassium dichromate (K(2)Cr(2)O(7)) treatment exhibited an increase in cell viability and decrease of DNA damage as compared to K(2)Cr(2)O(7) treatment alone, as evaluated by WST-8 and Comet assay respectively. Further, MCE administration 1h prior to graded doses of K(2)Cr(2)O(7) significantly decreased reactive oxygen species (ROS) level, increased the mitochondrial membrane potential, reduced apoptosis and caspase 3 activity. MCE also ameliorated K(2)Cr(2)O(7) induced decrease in superoxide dismutase (SOD), glutathione peroxidase (GPx) antioxidant enzyme levels in BEAS-2B and PBL cells accompanied by reduction in lipid peroxides with maximum effect at 50 μg/ml. Thus, this study provides strong evidence to support the beneficial effect of MCE in preventing Cr(VI) induced toxicity in BEAS-2B and PBL cells.
authors.affiliation: Environmental Biotechnology Division, National Environmental Engineering Research Institute, Nehru Marg, Nagpur 440020, India. dipaduttadeb@gmail.com
authors.forename: Dipanwita Dutta
authors.initials: DD
authors.lastname: Deb
authors.forename: G
authors.initials: G
authors.lastname: Parimala
authors.forename: S Saravana
authors.initials: SS
authors.lastname: Devi
authors.forename: T
authors.initials: T
authors.lastname: Chakrabarti
mesh_terms: D000975:Antioxidants / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D015415:Biomarkers / Q000378:metabolism; D029974:Carum / Q000737:chemistry*; D018929:Cell Culture Techniques; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D020552:Comet Assay; D004249:DNA Damage; D004785:Environmental Pollutants / Q000633:toxicity*; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D011192:Potassium Dichromate / Q000633:toxicity*; D012639:Seeds / Q000737:chemistry
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D004785:Environmental Pollutants; D010936:Plant Extracts; D011192:Potassium Dichromate