title: Modeled microgravity suppressed expansion of the MBP-specific T lymphocytes of rats with experimental autoimmune encephalomyelitis.
abstract: Experimental autoimmune encephalomyelitis (EAE) is characterized by uncontrolled proliferation of autoreactive T lymphocytes, with markedly increased secretion of pro-inflammatory cytokines. To further dissect the pathogenetic pathways of this disease, we exposed T lymphocytes from EAE rats, which were specific for myelin basic protein (MBP) to a modeled microgravity (MMG) environment, using a rotated cell culture system (RCCS) that was known to suppress proliferation of normal T cells. Following exposure to MMG, the proliferation of EAE lymphocytes decreased dramatically compared to those cultured in unit gravity (UG). At the beginning of MMG, a significant increase of apoptosis of MBP-specific T lymphocytes was observed, while at a later stage, the cytokine secretion profile of exposed MBP-specific T lymphocytes was altered, as was the differentiation of Th subsets. We concluded that the function of MBP-specific T lymphocytes was disordered after exposure to MMG.
authors.affiliation: Department of Neurobiology, Neurobiology Key Laboratory, Harbin Medical University, Education Department of Heilongjiang Province, China.
authors.forename: Shasha
authors.initials: S
authors.lastname: Bai
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.forename: Jinghua
authors.initials: J
authors.lastname: Wang
authors.forename: Dongxu
authors.initials: D
authors.lastname: Zhai
authors.forename: Qingfei
authors.initials: Q
authors.lastname: Kong
authors.forename: Yumei
authors.initials: Y
authors.lastname: Liu
authors.forename: Xijun
authors.initials: X
authors.lastname: Liu
authors.forename: Bo
authors.initials: B
authors.lastname: Sun
authors.forename: Jiayuan
authors.initials: J
authors.lastname: Xu
authors.forename: Dandan
authors.initials: D
authors.lastname: Wang
authors.forename: Guangyou
authors.initials: G
authors.lastname: Wang
authors.forename: Lili
authors.initials: L
authors.lastname: Mu
authors.forename: Xiangying
authors.initials: X
authors.lastname: Xu
authors.forename: Hulun
authors.initials: H
authors.lastname: Li
mesh_terms: D000818:Animals; D017209:Apoptosis; D020169:Caspases / Q000276:immunology / Q000378:metabolism; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology / Q000503:physiopathology*; D000939:Epitopes / Q000276:immunology; D005260:Female; D004676:Myelin Basic Protein / Q000276:immunology*; D051381:Rats; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000166:cytology / Q000276:immunology; D014893:Weightlessness
chemical_list: D016207:Cytokines; D000939:Epitopes; D004676:Myelin Basic Protein; D020169:Caspases