title: Bone marrow mesenchymal stem cells can improve the motor function of a Huntington's disease rat model.
abstract: OBJECTIVE
  The purpose of this study was to investigate the ability of bone marrow mesenchymal stem cells (BMSCs) to survive in the striatum and improve motor function in the quinolinic acid (QA) lesion rat model of Huntington's disease (HD).
  
  
  METHODS
  One week following QA lesioning of the striatum, rats were transplanted with BMSCs that had been expanded in culture and labeled with 4'-6-diamidino-2-phenylindole (DAPI) prior to transplantation.
  
  
  RESULTS
  BMSCs survived transplantation into the lesioned striatum and differentiated into both neurons. Moreover, transplantation of BMSCs significantly reduced motor dysfunction observed following striatal QA lesioning. Stereological striatal volume analyses performed on Nissl-stained sections revealed that rats transplanted with BMSCs had a greater striatal volume on the lesioned side compared with rats injected with vehicle. Cultured BMSCs expressed trophic factors, including nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), glial cell line-derived neurotrophic factor (GDNF) and ciliary neurotrophic factor (CNTF). In the striatum of BMSC-treated group, the expression levels of these genes were significantly elevated when compared with those of the control group.
  
  
  CONCLUSIONS
  Our data suggested that striatal transplants of BMSCs elicit behavioral and anatomical recovery in the QA lesion model of HD.
authors.affiliation: Division of Histology and Embryology, Department of Anatomy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Jiang
authors.forename: Hailong
authors.initials: H
authors.lastname: Lv
authors.forename: Shanshan
authors.initials: S
authors.lastname: Huang
authors.forename: Huiping
authors.initials: H
authors.lastname: Tan
authors.forename: Yinong
authors.initials: Y
authors.lastname: Zhang
authors.forename: He
authors.initials: H
authors.lastname: Li
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000502:physiology; D003342:Corpus Striatum / Q000473:pathology; D004195:Disease Models, Animal; D005786:Gene Expression Regulation / Q000502:physiology; D006816:Huntington Disease / Q000473:pathology / Q000503:physiopathology* / Q000601:surgery*; D007211:Indoles; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000378:metabolism; D009043:Motor Activity / Q000502:physiology*; D009414:Nerve Growth Factors / Q000235:genetics / Q000378:metabolism; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D045442:Rotarod Performance Test
chemical_list: D007211:Indoles; D009414:Nerve Growth Factors; D012333:RNA, Messenger; C007293:DAPI; D010751:Phosphopyruvate Hydratase