title: Photodynamic therapy and two-photon bio-imaging applications of hydrophobic chromophores through amphiphilic polymer delivery.
abstract: The synthesis and photophysical properties of two lipophilic quadrupolar chromophores featuring anthracenyl (1) or dibromobenzene (2) were described. These two chromophores combined significant two-photon absorption cross-sections with high fluorescence quantum yield for 1 and improved singlet oxygen generation efficiency for 2, in organic solvents. The use of Pluronic nanoparticles allowed a simple and straightforward introduction of these lipophilic chromophores into biological cell media. Their internal distribution in various cell lines was studied using fluorescence microscopy and flow-cytometry following a successful staining that was achieved upon 2 h of incubation. Finally, multiphoton excitation microscopy and photodynamic therapy capability of the chromophores were demonstrated by cell exposure to a 820 nm fs laser and cell death upon one photon resonant irradiation at 436 ± 10 nm, respectively.
authors.affiliation: Université de Lyon, CNRS UMR 5182, Institut de Chimie de Lyon, Université Lyon 1, Ecole Normale Supérieure de Lyon, Site Monod, 46 allée d'Italie, 69364, Lyon Cedex 07, France.
authors.forename: Thibault
authors.initials: T
authors.lastname: Gallavardin
authors.forename: Mathieu
authors.initials: M
authors.lastname: Maurin
authors.forename: Sophie
authors.initials: S
authors.lastname: Marotte
authors.forename: Timea
authors.initials: T
authors.lastname: Simon
authors.forename: Ana-Maria
authors.initials: AM
authors.lastname: Gabudean
authors.forename: Yann
authors.initials: Y
authors.lastname: Bretonnière
authors.forename: Mikael
authors.initials: M
authors.lastname: Lindgren
authors.forename: Frédéric
authors.initials: F
authors.lastname: Lerouge
authors.forename: Patrick L
authors.initials: PL
authors.lastname: Baldeck
authors.forename: Olivier
authors.initials: O
authors.lastname: Stéphan
authors.forename: Yann
authors.initials: Y
authors.lastname: Leverrier
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Marvel
authors.forename: Stéphane
authors.initials: S
authors.lastname: Parola
authors.forename: Olivier
authors.initials: O
authors.lastname: Maury
authors.forename: Chantal
authors.initials: C
authors.lastname: Andraud
mesh_terms: D000480:Alkynes / Q000737:chemistry* / Q000494:pharmacology / Q000627:therapeutic use; D000814:Aniline Compounds / Q000737:chemistry* / Q000494:pharmacology / Q000627:therapeutic use; D000818:Animals; D000873:Anthracenes / Q000737:chemistry* / Q000494:pharmacology / Q000627:therapeutic use; D017209:Apoptosis; D001969:Bromobenzenes / Q000737:chemistry* / Q000494:pharmacology / Q000627:therapeutic use; D045744:Cell Line, Tumor; D005456:Fluorescent Dyes / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D008856:Microscopy, Fluorescence; D053758:Nanoparticles / Q000737:chemistry; D009369:Neoplasms / Q000188:drug therapy; D010778:Photochemotherapy; D017785:Photons; D017319:Photosensitizing Agents / Q000737:chemistry* / Q000494:pharmacology / Q000627:therapeutic use; D020442:Poloxamer / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D011789:Quantum Theory; D051381:Rats; D026082:Singlet Oxygen / Q000378:metabolism
chemical_list: D000480:Alkynes; D000814:Aniline Compounds; D000873:Anthracenes; D001969:Bromobenzenes; D005456:Fluorescent Dyes; D017319:Photosensitizing Agents; D011108:Polymers; D020442:Poloxamer; D026082:Singlet Oxygen