title: Robusta coffee beans post-harvest microflora: Lactobacillus plantarum sp. as potential antagonist of Aspergillus carbonarius.
abstract: Coffee contamination by ochratoxigenic fungi affects both coffee quality as well as coffee price with harmful consequences on the economy of the coffee exporting countries for whom which is their main source of income. Fungal strains were isolated from coffee beans and identified as black Aspergilli. Ochratoxigenic moulds like Aspergillus carbonarius were screened and selected for detailed studies. Also lactic acid bacteria (LAB) were isolated from silage coffee pulp and their antifungal activity was tested on dual-culture agar plate. Ten of the isolated LAB demonstrated antifungal effect against A. carbonarius. API 50 CH and APIZYM were used to perform phenotypic identification. 16S rDNA sequencing was made to confirm the results.
authors.affiliation: Institut Méditerranéen d'Écologie et de Paléoécologie (IMEP, UMR CNRS/IRD 6116), Laboratoire d'Écologie Microbienne et Biotechnologies, FST St Jérôme, Université Paul Cézanne, 13397 Marseille Cedex 20, France. olgitha@yahoo.fr
authors.forename: Olga
authors.initials: O
authors.lastname: Djossou
authors.forename: Isabelle
authors.initials: I
authors.lastname: Perraud-Gaime
authors.forename: Fatma Lakhal
authors.initials: FL
authors.lastname: Mirleau
authors.forename: Gabriela
authors.initials: G
authors.lastname: Rodriguez-Serrano
authors.forename: Germain
authors.initials: G
authors.lastname: Karou
authors.forename: Sebastien
authors.initials: S
authors.lastname: Niamke
authors.forename: Imene
authors.initials: I
authors.lastname: Ouzari
authors.forename: Abdellatif
authors.initials: A
authors.lastname: Boudabous
authors.forename: Sevastianos
authors.initials: S
authors.lastname: Roussos
mesh_terms: D001230:Aspergillus / Q000254:growth & development* / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D050260:Carbohydrate Metabolism; D040503:Coffea / Q000382:microbiology*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004798:Enzymes / Q000378:metabolism; D048191:Lactiplantibacillus plantarum / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism / Q000502:physiology*; D056265:Microbial Interactions; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012639:Seeds / Q000382:microbiology; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D004798:Enzymes; D012336:RNA, Ribosomal, 16S