title: Microarray-based evaluation of whole-community genome DNA amplification methods.
abstract: Three whole-community genome amplification methods, Bst, REPLI-g, and Templiphi, were evaluated using a microarray-based approach. The amplification biases of all methods were <3-fold. For pure-culture DNA, REPLI-g and Templiphi showed less bias than Bst. For community DNA, REPLI-g showed the least bias and highest number of genes, while Bst had the highest success rate and was suitable for low-quality DNA.
authors.affiliation: State Key Joint Laboratory of Environmental Stimulation and Pollution Control, School of Environment, Tsinghua University, Beijing, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: Joy D
authors.initials: JD
authors.lastname: Van Nostrand
authors.forename: Liyou
authors.initials: L
authors.lastname: Wu
authors.forename: Zhili
authors.initials: Z
authors.lastname: He
authors.forename: Guanghe
authors.initials: G
authors.lastname: Li
authors.forename: Jizhong
authors.initials: J
authors.lastname: Zhou
mesh_terms: D005827:Genetics, Microbial / Q000379:methods*; D056186:Metagenomics / Q000379:methods*; D046228:Microarray Analysis / Q000379:methods*