title: [Study on the mechanism of C17orf62 induced cell death].
abstract: OBJECTIVE
  To isolate the long coding sequence of human novel gene C17orf62 which is named as C17orf62-L by us, and analyze its effects on cell viability, subcellular localization, and expression profile in multiple cell lines.
  
  
  METHODS
  RT-PCR (reverse transcription polymerase chain reaction ) was used to clone C17orf62-L which encoded 187 amino acids from human multi-tissue cDNA library. We used bioinformatics analysis to identify structural characteristics of C17orf62-L, and RT-PCR to detect its expression. By Laser Scanning Confocal Microscopy we identified its subcellular localization of C17orf62-L. Furthermore, flow cytometry experiment was used to validate whether overexpression of C17orf62-L could influence cell phenotypes and Western blot was used to study related mechanisms.
  
  
  RESULTS
  C17orf62-L was cloned and constructed into the pcDNA-and pEGFP-expression plasmids. C17orf62-L had signal peptide and transmembrane domain.C17orf62-L was widely expressed in multiple cell lines and was validated partial co-localization with Golgi apparatus. Functional studies showed C17orf62-L could induce cell death accompanied with rising of cleaved PARP(poly ADP-ribose polymerase).
  
  
  CONCLUSIONS
  Human C17orf62 is a novel cell death inducing gene.
authors.affiliation: Department of Medical Immunology, Peking University School of Basic Medical Science, Peking University Center for Human Disease Genomics, Beijing, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Deng
authors.forename: Hong-shan
authors.initials: HS
authors.lastname: Zhao
authors.forename: Zhi
authors.initials: Z
authors.lastname: Peng
authors.forename: Wei-wei
authors.initials: WW
authors.lastname: Deng
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.forename: Shuai
authors.initials: S
authors.lastname: Guo
authors.forename: Tai-ping
authors.initials: TP
authors.lastname: Shi
mesh_terms: D001798:Blood Proteins / Q000235:genetics* / Q000502:physiology; D016923:Cell Death / Q000235:genetics*; D002886:Chromosomes, Human, Pair 17 / Q000235:genetics*; D003001:Cloning, Molecular; D016207:Cytokines / Q000235:genetics* / Q000502:physiology; D006801:Humans; D016366:Open Reading Frames / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
chemical_list: D001798:Blood Proteins; C412483:CYTL1 protein, human; D016207:Cytokines