title: Cross-talk of the related bioactivity mediators in serum after injection of soluble TNF-α receptor on silicosis model of rats.
abstract: This study investigates cross-talk of the related bioactivity mediators in silica-induced pulmonary inflammatory and fibrosis on rats, which contributes to the preventive and therapeutic effect of soluble TNF-α receptor. Wistar rats received saline or 50 mg of quartz by intratracheal instillation. Rats in drug-treated groups were given soluble tumor necrosis factor-α (TNF-α) receptor (500 μg) by hypodermic injection on days 1, 5 and 8 after operation. At 7 days or 14 days after instillation, rats were killed to observe the degree of injury and expression of the related bioactivity mediators including nuclear factor KB (NF-KB), nitric oxide, interleukin-1β, interleukin-10, transforming growth factor beta 1 (TGF-β1), TNF-α, interferon-Y (IFN-Y) and granulocyte macrophage colony-stimulating factor (GM-CSF). The area percentages of type I and III collagens in intervention group were lower than those in silica group. The expression of NF-κB, TGF-β1, and COL I were lower in intervention group than in silica group(p < 0.05) and GM-CSF was significantly higher (p < 0.05) at 7 days after instillation, however, NF-κB, TGF-β1, and COL I were identically lower in intervention group than in silica group, and TNF-α, IFN-γ, and GM-CSF were higher at 14 days after instillation. It may be concluded that soluble TNF-α receptor upregulating or downregulating the expression of the related bioactivity mediators results in decreasing lung injury induced by silica.
authors.affiliation: Department of Hygiene, Medical College of China People's Armed Police Force, Tianjin, People's Republic of China, Key Laboratory of Biomarker for Occupational and Environmental Hazard, Tianjin, People's Republic of China. gaohs_LL@yahoo.com.cn
authors.forename: Hong-Sheng
authors.initials: HS
authors.lastname: Gao
authors.lastname: Rong Xue
authors.lastname: Peng Ding
authors.forename: Nan-Fang
authors.initials: NF
authors.lastname: Chen
authors.lastname: Bing Meng
authors.forename: Hua-Bing
authors.initials: HB
authors.lastname: Zhao
authors.lastname: Zhen Yang
authors.forename: Shi-Xing
authors.initials: SX
authors.lastname: Wang
mesh_terms: D000818:Animals; D003094:Collagen / Q000097:blood; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000097:blood; D007371:Interferon-gamma / Q000097:blood; D016753:Interleukin-10 / Q000097:blood; D053583:Interleukin-1beta / Q000097:blood; D008297:Male; D016328:NF-kappa B / Q000097:blood; D009569:Nitric Oxide / Q000097:blood; D051381:Rats; D017208:Rats, Wistar; D047888:Receptors, Tumor Necrosis Factor, Type I / Q000097:blood*; D015398:Signal Transduction; D012822:Silicon Dioxide / Q000633:toxicity*; D012829:Silicosis / Q000188:drug therapy*; D053773:Transforming Growth Factor beta1 / Q000097:blood
chemical_list: D053583:Interleukin-1beta; D016328:NF-kappa B; D047888:Receptors, Tumor Necrosis Factor, Type I; D053773:Transforming Growth Factor beta1; D016753:Interleukin-10; D009569:Nitric Oxide; D012822:Silicon Dioxide; D007371:Interferon-gamma; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D003094:Collagen