title: Absence of human papillomavirus in patients with breast cancer in north-west China.
abstract: The purpose of this study was to investigate the human papillomavirus (HPV) infection rate in malignant and benign breast tumours in north-west Chinese patients. Unselected fresh tissue samples were extracted from 48 invasive breast carcinoma and 30 benign breast tumours between August 2009 and August 2010. We used real-time fluorescence quantitative PCR (FQ-PCR) and in situ hybridization to detect HPV 6, 11 and 16, 18 sequences targeting the E6, E7 open-reading frame. The presence of HPV 6, 11 was only demonstrated in the 3 (10%) benign phyllodes tumour specimens. No HPV infection was detected in all the 48 invasive breast carcinoma. Specifically, all the positive specimens were detected by in situ hybridization but not in FQ-PCR. In addition, we found that the positive specimens became negative after 3 months frozen at -70°C. HPV was absent in patients with breast tumours in north-west China.
authors.affiliation: Department of Vascular and Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, 17th Changle West Road, Xi'an, 710032 Shaanxi Province, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Chang
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
authors.forename: Qing
authors.initials: Q
authors.lastname: Yao
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Lv
authors.forename: Juliang
authors.initials: J
authors.lastname: Zhang
authors.forename: Wen
authors.initials: W
authors.lastname: Guo
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.forename: Jianghao
authors.initials: J
authors.lastname: Chen
mesh_terms: D000328:Adult; D001943:Breast Neoplasms / Q000235:genetics / Q000473:pathology / Q000821:virology*; D002681:China; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D005453:Fluorescence; D006801:Humans; D017403:In Situ Hybridization; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D009856:Oncogene Proteins, Viral / Q000235:genetics; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics*; D050725:Papillomavirus E7 Proteins / Q000235:genetics; D030361:Papillomavirus Infections / Q000235:genetics / Q000821:virology*; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction; D012097:Repressor Proteins / Q000235:genetics
chemical_list: D004279:DNA, Viral; C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D012097:Repressor Proteins