title: The ADP-ribose-1''-monophosphatase domains of severe acute respiratory syndrome coronavirus and human coronavirus 229E mediate resistance to antiviral interferon responses.
abstract: Several plus-strand RNA viruses encode proteins containing macrodomains. These domains possess ADP-ribose-1″-phosphatase (ADRP) activity and/or bind poly(ADP-ribose), poly(A) or poly(G). The relevance of these activities in the viral life cycle has not yet been resolved. Here, we report that genetically engineered mutants of severe acute respiratory syndrome coronavirus (SARS-CoV) and human coronavirus 229E (HCoV-229E) expressing ADRP-deficient macrodomains displayed an increased sensitivity to the antiviral effect of alpha interferon compared with their wild-type counterparts. The data suggest that macrodomain-associated ADRP activities may have a role in viral escape from the innate immune responses of the host.
authors.affiliation: Department of Virology, University of Freiburg, D-79008 Freiburg, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Kuri
authors.affiliation: Research Department, Kantonal Hospital St Gallen, St Gallen, Switzerland.
authors.forename: Klara K
authors.initials: KK
authors.lastname: Eriksson
authors.affiliation: Institute of Virology and Immunology, University of Würzburg, D-97078 Würzburg, Germany.
authors.forename: Akos
authors.initials: A
authors.lastname: Putics
authors.affiliation: Research Department, Kantonal Hospital St Gallen, St Gallen, Switzerland.
authors.forename: Roland
authors.initials: R
authors.lastname: Züst
authors.affiliation: Molecular Virology Laboratory, Department of Medical Microbiology, Center of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands.
authors.forename: Eric J
authors.initials: EJ
authors.lastname: Snijder
authors.affiliation: Division of Virology, Department of Cellular and Molecular Medicine, School of Medical and Veterinary Sciences, University of Bristol, Bristol, UK.
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Davidson
authors.affiliation: Division of Virology, Department of Cellular and Molecular Medicine, School of Medical and Veterinary Sciences, University of Bristol, Bristol, UK.
authors.forename: Stuart G
authors.initials: SG
authors.lastname: Siddell
authors.affiliation: Research Department, Kantonal Hospital St Gallen, St Gallen, Switzerland.
authors.forename: Volker
authors.initials: V
authors.lastname: Thiel
authors.affiliation: Institute of Medical Virology, Justus Liebig University Giessen, D-35392 Giessen, Germany.
authors.forename: John
authors.initials: J
authors.lastname: Ziebuhr
authors.affiliation: Institute for Virology, Philipps-University Marburg, D-35043 Marburg, Germany.|Department of Virology, University of Freiburg, D-79008 Freiburg, Germany.
authors.forename: Friedemann
authors.initials: F
authors.lastname: Weber
mesh_terms: D000595:Amino Acid Sequence; D000998:Antiviral Agents / Q000276:immunology*; D002460:Cell Line; D028941:Coronavirus 229E, Human / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics / Q000276:immunology; D018352:Coronavirus Infections / Q000235:genetics / Q000276:immunology* / Q000821:virology; D006801:Humans; D016898:Interferon-alpha / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D010744:Phosphoric Monoester Hydrolases / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D017434:Protein Structure, Tertiary; D045473:Severe acute respiratory syndrome-related coronavirus / Q000737:chemistry / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D016415:Sequence Alignment; D045169:Severe Acute Respiratory Syndrome / Q000235:genetics / Q000276:immunology* / Q000821:virology; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology
chemical_list: D000998:Antiviral Agents; D016898:Interferon-alpha; D014764:Viral Proteins; D010744:Phosphoric Monoester Hydrolases