title: Detection of higher levels of dengue viremia using FcγR-expressing BHK-21 cells than FcγR-negative cells in secondary infection but not in primary infection.
abstract: It has been reported that levels of viremia reflect the severity of illness in dengue virus infection. We assessed the levels of viremia in patients with primary and secondary infections, using 2 cell lines: FcγR-expressing BHK cells and FcγR-negative cells. In primary infection, virus titers were at similar levels between FcγR-expressing and FcγR-negative cells. In secondary infection, however, virus titers were ∼10 times higher in FcγR-expressing cells on days 1-6 when compared with FcγR-negative cells, indicating discrepancy in viremia titers between FcγR-expressing and FcγR-negative cells. The results suggest that dengue virus-antibody complexes with infectious capacity exist in patients with secondary infection, and these immune complexes can be detected by using FcγR-expressing cells. As it has been reported that principal target cells of dengue virus infection are FcγR-positive, monocyte/macrophage lineage cells, virus titers determined using FcγR-expressing cells may better reflect the actual viremic conditions in vivo.
authors.affiliation: Department of Virology 1, National Institute of Infectious Diseases, Tokyo, Japan.
authors.forename: Meng Ling
authors.initials: ML
authors.lastname: Moi
authors.forename: Chang-Kweng
authors.initials: CK
authors.lastname: Lim
authors.forename: Akira
authors.initials: A
authors.lastname: Kotaki
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Takasaki
authors.forename: Ichiro
authors.initials: I
authors.lastname: Kurane
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D000936:Antigen-Antibody Complex / Q000032:analysis* / Q000502:physiology; D002460:Cell Line; D006224:Cricetinae; D003715:Dengue / Q000097:blood / Q000276:immunology* / Q000821:virology; D003716:Dengue Virus / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000276:immunology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D012367:RNA, Viral / Q000032:analysis; D017452:Receptors, IgG / Q000235:genetics / Q000378:metabolism*; D012008:Recurrence; D020133:Reverse Transcriptase Polymerase Chain Reaction; D010948:Viral Plaque Assay; D014766:Viremia / Q000097:blood / Q000276:immunology* / Q000821:virology; D055815:Young Adult
chemical_list: D000936:Antigen-Antibody Complex; C080568:Fc gamma receptor IIA; D012367:RNA, Viral; D017452:Receptors, IgG