title: Upregulation of collagen type 1 in aged murine dermis after transplantation of dermal multipotent cells.
abstract: BACKGROUND
  Multipotent cells can be isolated from dermis, and have wound-healing and antioxidant effects on human and murine skin.
  
  
  OBJECTIVE
  To investigate the changes in aged dermis after injection of dermal multipotent cells and adult fibroblasts.
  
  
  METHODS
  Dermal multipotent cells were isolated and cultured from 3-day-old BALB/c mice by a regular dermal fibroblast culture system. We used various inducers to confirm the multilineage differentiation of these cells, then the dermal multipotent cells were transplanted into the dermis of aged (12-week-old) BALB/c mice. At 2 and 4 weeks after transplantation, we examined dermal thickness, and determined the amounts of collagen type I present, using PCR and western blotting.
  
  
  RESULTS
  The dermal multipotent cells exhibited adipogenic and osteogenic phenotypes when cultured in the presence of certain inducers. Dermal thickness and collagen content in the dermis also increased in the mice injected with dermal multipotent cells, which also had increased RNA and protein expression of collagen type I.
  
  
  CONCLUSIONS
  Collectively, these results indicate that dermal multipotent cells are more effective than fibroblasts in increasing collagen type I and dermal thickness, which may be of importance in preventing skin ageing.
authors.affiliation: Department of Dermatology, West China Hospital, Sichuan University, Chengdu, China.
authors.forename: S
authors.initials: S
authors.lastname: Xue
authors.forename: L
authors.initials: L
authors.lastname: Li
mesh_terms: D000818:Animals; D015153:Blotting, Western; D024042:Collagen Type I / Q000378:metabolism*; D020405:Dermis / Q000166:cytology / Q000378:metabolism*; D005347:Fibroblasts / Q000637:transplantation*; D051379:Mice; D008807:Mice, Inbred BALB C; D039902:Multipotent Stem Cells / Q000637:transplantation*; D016133:Polymerase Chain Reaction; D015595:Skin Aging / Q000502:physiology*; D015854:Up-Regulation
chemical_list: D024042:Collagen Type I