title: Performance of the BD GeneOhm MRSA achromopeptidase assay for real-time PCR detection of methicillin-resistant Staphylococcus aureus in nasal specimens.
abstract: We evaluated the BD GeneOhm MRSA achromopeptidase (ACP) assay, which incorporates a new specimen preparation approach. A total of 1,216 leftover nasal samples were tested; using culture as the gold standard, the sensitivity and specificity were 92% and 94.6%, respectively. The new lysis method provides good sensitivity and simplifies specimen preparation.
authors.affiliation: NorthShore University HealthSystem, Evanston, Illinois 60201, USA. ppatel@northshore.org
authors.forename: Parul A
authors.initials: PA
authors.lastname: Patel
authors.forename: Nathan A
authors.initials: NA
authors.lastname: Ledeboer
authors.forename: Christine C
authors.initials: CC
authors.lastname: Ginocchio
authors.forename: Susan
authors.initials: S
authors.lastname: Condon
authors.forename: Stephanie
authors.initials: S
authors.lastname: Bouchard
authors.forename: Peibing
authors.initials: P
authors.lastname: Qin
authors.forename: Tobi
authors.initials: T
authors.lastname: Karchmer
authors.forename: Lance R
authors.initials: LR
authors.lastname: Peterson
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D001431:Bacteriological Techniques / Q000379:methods*; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009297:Nasal Mucosa / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D012697:Serine Endopeptidases / Q000235:genetics*; D013048:Specimen Handling / Q000379:methods; D013203:Staphylococcal Infections / Q000175:diagnosis* / Q000382:microbiology
chemical_list: D001426:Bacterial Proteins; D011933:Reagent Kits, Diagnostic; D012697:Serine Endopeptidases; C036813:lysyl endopeptidase