title: A microfluidic device for simultaneous electrical and mechanical measurements on single cells.
abstract: This paper presents a microfluidic device for simultaneous mechanical and electrical characterization of single cells. The device performs two types of cellular characterization (impedance spectroscopy and micropipette aspiration) on a single chip to enable cell electrical and mechanical characterization. To investigate the performance of the device design, electrical and mechanical properties of MC-3T3 osteoblast cells were measured. Based on electrical models, membrane capacitance of MC-3T3 cells was determined to be 3.39±1.23 and 2.99±0.82 pF at the aspiration pressure of 50 and 100 Pa, respectively. Cytoplasm resistance values were 110.1±37.7 kΩ (50 Pa) and 145.2±44.3 kΩ (100 Pa). Aspiration length of cells was found to be 0.813±0.351 μm at 50 Pa and 1.771±0.623 μm at 100 Pa. Quantified Young's modulus values were 377±189 Pa at 50 Pa and 344±156 Pa at 100 Pa. Experimental results demonstrate the device's capability for characterizing both electrical and mechanical properties of single cells.
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.forename: Yi
authors.initials: Y
authors.lastname: Zheng
authors.forename: Qingyuan
authors.initials: Q
authors.lastname: Tan
authors.forename: Yan Liang
authors.initials: YL
authors.lastname: Zhang
authors.forename: Jason
authors.initials: J
authors.lastname: Li
authors.forename: William R
authors.initials: WR
authors.lastname: Geddie
authors.forename: Michael A S
authors.initials: MA
authors.lastname: Jewett
authors.forename: Yu
authors.initials: Y
authors.lastname: Sun