title: Capillary electrophoresis immunoassays with conjugated quantum dots.
abstract: Water-soluble CdTe quantum dots (QDs) and their conjugates with antibodies and antigenes were prepared by optimized procedures for applications in CE immunoassays. The QD size of 3.5 nm, excitation spectrum in the range of 300-500 nm, the maximum wavelength of the emission spectrum at 610 nm, quantum yield of 0.25 and luminescence lifetimes in the range of 3.6-43 ns were determined. The 0.1 M solution of TRIS/TAPS (pH 8.3) was found to be the optimum buffer for the separation of the antiovalbumin-ovalbumin immunocomplex from the free conjugates of QDs.
authors.affiliation: Institute of Analytical Chemistry, Czech Academy of Sciences, Brno, Czech Republic. klep@iach.cz
authors.forename: Karel
authors.initials: K
authors.lastname: Klepárník
authors.forename: Ivona
authors.initials: I
authors.lastname: Voráčová
authors.forename: Marcela
authors.initials: M
authors.lastname: Lišková
authors.forename: Jan
authors.initials: J
authors.lastname: Přikryl
authors.forename: Věra
authors.initials: V
authors.lastname: Hezinová
authors.forename: František
authors.initials: F
authors.lastname: Foret
mesh_terms: D019187:Cadmium Compounds / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D007118:Immunoassay / Q000379:methods*; D008163:Luminescent Measurements; D010047:Ovalbumin / Q000737:chemistry; D010316:Particle Size; D045663:Quantum Dots; D013691:Tellurium / Q000737:chemistry
chemical_list: D019187:Cadmium Compounds; D010047:Ovalbumin; D013691:Tellurium; C028337:cadmium telluride