title: A novel biosensing mechanism based on a poly(N-butyl benzimidazole)-modified gold electrode for the detection of hydrogen peroxide.
abstract: A novel mechanism to detect hydrogen peroxide (H(2)O(2)) using a poly(N-butyl benzimidazole) (PBBI)-modified gold (PBBI/Au) electrode is proposed. Synthetic PBBI was oxidized using a mixture of acetic acid (AcOH) and H(2)O(2) to form PBBI N-oxide (PBBINO). The structure of PBBINO was verified by Fourier transform infrared spectroscopy (FT-IR) and the degree of oxidation was measured by X-ray photoelectron spectroscopy (XPS). Moreover, the oxide could be reduced electrochemically back to PBBI. Based on this reaction, a novel enzyme-free PBBI/Au electrode was developed to detect H(2)O(2) in the presence of AcOH electrochemically. The biosensor detected H(2)O(2) linearly over concentrations ranging from 25 μM to 10 mM with a detection limit of 6.25 μM in phosphate buffer solution (PBS) mixed with AcOH at pH 6.4. In addition, at an applied potential of -0.5 V, the sensor characteristics could be tuned using AcOH over a pH range of 3.7-6.4. The sensitivity of the probe could be enhanced from 35.1 to 419.4 μA mM(-1) cm(-2) by modifying the surface morphology of the PBBI/Au electrode from a smooth plane to a granular, three-dimensional configuration. Furthermore, it was not influenced by interfering compounds and showed high thermal stability.
authors.affiliation: Green Technology Research Center, Department of Chemical and Materials Engineering, Chang Gung University, Tao-Yuan, Taiwan, ROC. huamy@mail.cgu.edu.tw
authors.forename: Mu-Yi
authors.initials: MY
authors.lastname: Hua
authors.forename: Hsiao-Chien
authors.initials: HC
authors.lastname: Chen
authors.forename: Rung-Ywan
authors.initials: RY
authors.lastname: Tsai
authors.forename: Yu-Chen
authors.initials: YC
authors.lastname: Lin
authors.forename: Leeyih
authors.initials: L
authors.lastname: Wang
mesh_terms: D019342:Acetic Acid / Q000737:chemistry; D001562:Benzimidazoles / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D055664:Electrochemical Techniques / Q000379:methods; D004566:Electrodes; D006046:Gold / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D010084:Oxidation-Reduction; D011108:Polymers / Q000737:chemistry*; D014527:Uric Acid / Q000737:chemistry
chemical_list: D001562:Benzimidazoles; D011108:Polymers; D014527:Uric Acid; D006046:Gold; D006861:Hydrogen Peroxide; D019342:Acetic Acid