title: Toward high-content/high-throughput imaging and analysis of embryonic morphogenesis.
abstract: In vivo study of embryonic morphogenesis tremendously benefits from recent advances in live microscopy and computational analyses. Quantitative and automated investigation of morphogenetic processes opens the field to high-content and high-throughput strategies. Following experimental workflow currently developed in cell biology, we identify the key challenges for applying such strategies in developmental biology. We review the recent progress in embryo preparation and manipulation, live imaging, data registration, image segmentation, feature computation, and data mining dedicated to the study of embryonic morphogenesis. We discuss a selection of pioneering studies that tackled the current methodological bottlenecks and illustrated the investigation of morphogenetic processes in vivo using quantitative and automated imaging and analysis of hundreds or thousands of cells simultaneously, paving the way for high-content/high-throughput strategies and systems analysis of embryonic morphogenesis.
authors.affiliation: California Institute of Technology, Division of Biology, Beckman Institute, Pasadena, California 91125, USA.
authors.forename: Thai V
authors.initials: TV
authors.lastname: Truong
authors.forename: Willy
authors.initials: W
authors.lastname: Supatto
mesh_terms: D000818:Animals; D004330:Drosophila / Q000196:embryology; D047108:Embryonic Development; D057166:High-Throughput Screening Assays; D007091:Image Processing, Computer-Assisted; D057054:Molecular Imaging; D009024:Morphogenesis / Q000502:physiology; D013194:Staining and Labeling