title: Rapid identification of bacteria with a disposable colorimetric sensing array.
abstract: Rapid identification of both species and even specific strains of human pathogenic bacteria grown on standard agar has been achieved from the volatiles they produce using a disposable colorimetric sensor array in a Petri dish imaged with an inexpensive scanner. All 10 strains of bacteria tested, including Enterococcus faecalis and Staphylococcus aureus and their antibiotic-resistant forms, were identified with 98.8% accuracy within 10 h, a clinically important time frame. Furthermore, the colorimetric sensor arrays also proved useful as a simple research tool for the study of bacterial metabolism and as an easy method for the optimization of bacterial production of fine chemicals or other fermentation processes.
authors.affiliation: Department of Applied Chemistry, National University of Kaohsiung, 700 Kaohsiung University Road, Kaosiung 811, Taiwan. jcarey@nuk.edu.tw
authors.forename: James R
authors.initials: JR
authors.lastname: Carey
authors.forename: Kenneth S
authors.initials: KS
authors.lastname: Suslick
authors.forename: Keren I
authors.initials: KI
authors.lastname: Hulkower
authors.forename: James A
authors.initials: JA
authors.lastname: Imlay
authors.forename: Karin R C
authors.initials: KR
authors.lastname: Imlay
authors.forename: Crystal K
authors.initials: CK
authors.lastname: Ingison
authors.forename: Jennifer B
authors.initials: JB
authors.lastname: Ponder
authors.forename: Avijit
authors.initials: A
authors.lastname: Sen
authors.forename: Aaron E
authors.initials: AE
authors.lastname: Wittrig
mesh_terms: D001419:Bacteria / Q000737:chemistry* / Q000145:classification* / Q000166:cytology; D015373:Bacterial Typing Techniques / Q000379:methods*; D003124:Colorimetry / Q000295:instrumentation / Q000379:methods*; D004209:Disposable Equipment; D013293:Enterococcus faecalis / Q000737:chemistry / Q000166:cytology; D006801:Humans; D046228:Microarray Analysis; D013211:Staphylococcus aureus / Q000737:chemistry / Q000166:cytology; D013997:Time Factors