title: Hsp60 and heme oxygenase-1 (Hsp32) in acute myocardial infarction.
abstract: Heat shock proteins (Hsps) are produced in response to various stressors, including ischemia-reperfusion, and they can exit cells and reach the blood. In this pilot study, we determined serum levels of Hsp60 and heme-oxygenase-1 (HO-1; also named Hsp32) in subjects with acute myocardial infarction (AMI) to assess their clinical significance and potential prognostic value. We also performed a bioinformatics analysis of the 2 molecules in search of structural clues on the mechanism of their release from cells. We studied 40 patients consecutively admitted for AMI (male:female patient ratio=20:20, mean age: 64 ± 13 years) and 40 matched controls. A blood sample was drawn for biochemical analyses within 24 h of symptoms onset, and Hsp60 and HO-1 concentrations were determined by enzyme-linked immunosorbent assay (ELISA). All patients were followed up for 6 months to register adverse post-AMI cardiovascular events. A multivariate analysis demonstrated that elevated Hsp60 (P=0.0361), creatine phosphokinase-muscle brain (CK-MB) (P=0.0446), and troponin (P=0.0490) were predictive of post-AMI adverse events. In contrast, increased HO-1 showed a significant association with less severity of coronary artery diseases (P=0.0223). These findings suggest that Hsp60 and HO-1 play distinct roles in the pathogenesis of AMI and subsequent AMI-related pathology. The possibility that these proteins differ in their roles and mechanisms of action in AMI and post-AMI pathology was supported also by the bioinformatics estimates of probability of their localization in various subcellular compartments. The results clear the way for subsequent investigation on the pathogenetic role and clinical significance of Hsp60 and HO-1 in AMI.
authors.affiliation: Department of Internal Medicine, Cardiovascular, and Nephrourological Diseases, University of Palermo, Palermo, Italy.
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Novo
authors.forename: Francesco
authors.initials: F
authors.lastname: Cappello
authors.forename: Manfredi
authors.initials: M
authors.lastname: Rizzo
authors.forename: Giovanni
authors.initials: G
authors.lastname: Fazio
authors.forename: Sabrina
authors.initials: S
authors.lastname: Zambuto
authors.forename: Enza
authors.initials: E
authors.lastname: Tortorici
authors.forename: Antonella
authors.initials: A
authors.lastname: Marino Gammazza
authors.forename: Antonella M
authors.initials: AM
authors.lastname: Gammazza
authors.forename: Simona
authors.initials: S
authors.lastname: Corrao
authors.forename: Giovanni
authors.initials: G
authors.lastname: Zummo
authors.forename: Everly C
authors.initials: EC
authors.lastname: De Macario
authors.forename: Alberto J L
authors.initials: AJ
authors.lastname: Macario
authors.forename: Pasquale
authors.initials: P
authors.lastname: Assennato
authors.forename: Salvatore
authors.initials: S
authors.lastname: Novo
authors.forename: Giovanni
authors.initials: G
authors.lastname: Li Volti
authors.forename: Giovanni L
authors.initials: GL
authors.lastname: Volti
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D018834:Chaperonin 60 / Q000097:blood; D019295:Computational Biology; D005260:Female; D005500:Follow-Up Studies; D051547:Heme Oxygenase-1 / Q000097:blood; D006801:Humans; D008297:Male; D008875:Middle Aged; D009203:Myocardial Infarction / Q000097:blood / Q000503:physiopathology; D010865:Pilot Projects; D011237:Predictive Value of Tests; D013223:Statistics as Topic
chemical_list: D018834:Chaperonin 60; C496541:HMOX1 protein, human; D051547:Heme Oxygenase-1