title: Screening and identification of host factors interacting with UL14 of herpes simplex virus 1.
abstract: The UL14 protein of herpes simplex virus type 1 (HSV-1) is highly conserved in herpesvirus family. However, its exact function during the HSV-1 replication cycle is little known. In the present study, a high throughput yeast two-hybrid system was employed to screen the cellular factors interacting with UL14, and five target candidates were yielded: (1) TSC22 domain family protein 3 (TSC22D3); (2) Mediator of RNA polymerase II transcription subunit 8 isoform 1(MED8); (3) Runt-related transcription factor 3 (RUNX3); (4) Arrestin beta-2 (ARRB2); (5) Cereblon (CRBN). Indirect immunofluorescent assay showed that both TSC22D3 and MED8 co-localized with UL14. Co-immunoprecipitation assay demonstrated that UL14 could be immunoprecipitated by TSC22D3, suggesting that UL14 interacted with TSC22D3 under physiological condition. In summary, this study opened up new avenues toward delineating the function and physiological significance of UL14 during the HSV-1 replication cycle.
authors.affiliation: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuchang, Wuhan, China.
authors.forename: Fuqing
authors.initials: F
authors.lastname: Wu
authors.forename: Junji
authors.initials: J
authors.lastname: Xing
authors.forename: Shuai
authors.initials: S
authors.lastname: Wang
authors.forename: Meili
authors.initials: M
authors.lastname: Li
authors.forename: Chunfu
authors.initials: C
authors.lastname: Zheng
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D019390:Arrestins / Q000235:genetics / Q000378:metabolism; D019556:COS Cells; D002522:Chlorocebus aethiops; D050677:Core Binding Factor Alpha 3 Subunit / Q000235:genetics / Q000378:metabolism; D019084:Fluorescent Antibody Technique, Indirect; D057809:HEK293 Cells; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D054884:Host-Pathogen Interactions; D006801:Humans; D047468:Immunoprecipitation; D056892:Mediator Complex / Q000235:genetics / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000235:genetics / Q000378:metabolism; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D044767:Ubiquitin-Protein Ligases; D014709:Vero Cells; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D014779:Virus Replication; D000071560:beta-Arrestin 2; D000071557:beta-Arrestins
chemical_list: C000605254:ARRB2 protein, human; D048868:Adaptor Proteins, Signal Transducing; D019390:Arrestins; C502543:CRBN protein, human; D050677:Core Binding Factor Alpha 3 Subunit; C480227:MED8 protein, human; D056892:Mediator Complex; C493729:Runx3 protein, human; C404226:TSC22D3 protein, human; D014157:Transcription Factors; C402639:UL14 protein, Human herpesvirus 1; D014764:Viral Proteins; D000071560:beta-Arrestin 2; D000071557:beta-Arrestins; D044767:Ubiquitin-Protein Ligases; D010447:Peptide Hydrolases