title: Liver injury and its molecular mechanisms in mice caused by exposure to cerium chloride.
abstract: Cerium has been demonstrated to damage liver of mice, but very little is known about the molecular mechanisms underlying the mouse liver apoptosis. In order to understand the liver injury induced by intragastric administration of cerium chloride (CeCl3) for 60 consecutive days, the hepatocyte ultrasrtucture, various oxidative stress parameters, and the stress-related gene expression levels were investigated for the mouse liver. The results demonstrated that CeCl3 had an obvious accumulation in the mouse liver, leading to a classical laddering cleavage of DNA and hepatocyte apoptosis. CeCl3 significantly promoted the accumulation of reactive oxygen species and inhibited the stress-related gene expression of superoxide dismutase, catalase, glutathione peroxidase, metallothionein, heat-shock protein 70, glutathione-S-transferase, P53, and transferring, and it effectively activated the cytochrome p450 1A. It implied that CeCl3 resulted in apoptosis and alteration of expression levels of the genes related with metal detoxification/metabolism regulation and radical scavenging action in mice.
authors.affiliation: Medical College of Soochow University, Suzhou, 215123, People's Republic of China.
authors.forename: Haiquan
authors.initials: H
authors.lastname: Zhao
authors.forename: Jie
authors.initials: J
authors.lastname: Cheng
authors.forename: Jingwei
authors.initials: J
authors.lastname: Cai
authors.forename: Zhe
authors.initials: Z
authors.lastname: Cheng
authors.forename: Yaling
authors.initials: Y
authors.lastname: Cui
authors.forename: Guodong
authors.initials: G
authors.lastname: Gao
authors.forename: Renping
authors.initials: R
authors.lastname: Hu
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Gong
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.forename: Fashui
authors.initials: F
authors.lastname: Hong
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects; D015415:Biomarkers / Q000378:metabolism; D002563:Cerium / Q000493:pharmacokinetics / Q000633:toxicity*; D056486:Chemical and Drug Induced Liver Injury / Q000378:metabolism / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D004785:Environmental Pollutants / Q000493:pharmacokinetics / Q000633:toxicity*; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D008658:Inactivation, Metabolic; D015227:Lipid Peroxidation; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D018384:Oxidative Stress; D011897:Random Allocation; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D004785:Environmental Pollutants; D017382:Reactive Oxygen Species; D002563:Cerium; C026690:cerous chloride