title: Measurements of cardiac ion channel subunits in the chronic atrioventricular block dog.
abstract: The chronic atrioventricular block (CAVB) dog has been widely used as an in vivo proarrhythmia model. mRNA levels of K(+) and Ca(2+) channels in the isolated ventricular tissues from normal and CAVB dogs were assayed using a real-time PCR. The mRNA levels of KvLQT1 and MiRP1 were significantly less in the CAVB heart compared with those in the intact heart, whereas no significant difference was detected in the mRNA levels of other K(+)- or Ca(2+)-channel subunits. Adaptation against chronic bradycardia-related pathophysiology may have decreased the mRNA levels of cardiac K(+) channels, which may partly explain the arrhythmogenic property of this model.
authors.affiliation: Department of Clinical Pharmacy, Faculty of Pharmaceutical Sciences, Toho University, Funabashi, Chiba 274-8510, Japan. akirat@phar.toho-u.ac.jp
authors.forename: Akira
authors.initials: A
authors.lastname: Takahara
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Wagatsuma
authors.forename: Shizuka
authors.initials: S
authors.lastname: Aritomi
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Konda
authors.forename: Yasuki
authors.initials: Y
authors.lastname: Akie
authors.forename: Yuji
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Atsushi
authors.initials: A
authors.lastname: Sugiyama
mesh_terms: D000818:Animals; D054537:Atrioventricular Block / Q000378:metabolism*; D001919:Bradycardia / Q000378:metabolism; D015220:Calcium Channels / Q000235:genetics / Q000378:metabolism*; D004285:Dogs; D015536:Down-Regulation; D005260:Female; D006352:Heart Ventricles / Q000378:metabolism; D051657:KCNQ1 Potassium Channel / Q000235:genetics / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D015221:Potassium Channels / Q000235:genetics / Q000378:metabolism*; D024642:Potassium Channels, Voltage-Gated / Q000235:genetics / Q000378:metabolism; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D015220:Calcium Channels; D051657:KCNQ1 Potassium Channel; D015221:Potassium Channels; D024642:Potassium Channels, Voltage-Gated; D021122:Protein Subunits; D012333:RNA, Messenger