title: The efficacy of HGAL and LMO2 in the separation of lymphomas derived from small B cells in nodal and extranodal sites, including the bone marrow.
abstract: We studied the efficacy of 2 germinal center B-cell markers, HGAL and LMO2, in the separation of lymphomas derived from small B cells, particularly follicular lymphoma (FL) and marginal zone lymphoma occurring in nodal, extranodal, splenic, and bone marrow sites using immunohistochemical analysis for CD10, BCL6, BCL2, HGAL, and LMO2. Our results showed that HGAL and LMO2 are sensitive and specific markers for detecting FL in nodal and extranodal sites. In contrast, all markers were down-regulated in FL infiltrates in the bone marrow. CD10 and HGAL were expressed in a subset of FLs in the bone marrow and were highly correlated with each other and with CD21, a marker of follicular dendritic cells. We conclude that HGAL and LMO2 should be considered in immunohistochemical panels used for the routine workup of lymphomas derived from small B cells. In the bone marrow, staining for HGAL or CD10 can be helpful in making a diagnosis of FL, although they are absent in a subset of cases.
authors.affiliation: Dept of Pathology, Division of Oncology, Stanford University School of Medicine, CA 94305, USA.
authors.forename: Sheren F
authors.initials: SF
authors.lastname: Younes
authors.forename: Andrew H
authors.initials: AH
authors.lastname: Beck
authors.forename: Robert S
authors.initials: RS
authors.lastname: Ohgami
authors.forename: Izidore S
authors.initials: IS
authors.lastname: Lossos
authors.forename: Ronald
authors.initials: R
authors.lastname: Levy
authors.forename: Roger A
authors.initials: RA
authors.lastname: Warnke
authors.forename: Yasodha
authors.initials: Y
authors.lastname: Natkunam
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D001402:B-Lymphocytes / Q000473:pathology; D014408:Biomarkers, Tumor; D001853:Bone Marrow / Q000473:pathology; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D018858:Germinal Center / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D047908:Intracellular Signaling Peptides and Proteins; D060588:LIM Domain Proteins; D008198:Lymph Nodes / Q000473:pathology; D018442:Lymphoma, B-Cell, Marginal Zone / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D008224:Lymphoma, Follicular / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D008667:Metalloproteins / Q000096:biosynthesis / Q000235:genetics*; D008840:Microfilament Proteins; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics*; D015260:Neprilysin / Q000378:metabolism; D009928:Organ Specificity; D011518:Proto-Oncogene Proteins; D013154:Spleen / Q000473:pathology
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; C476824:GCSAM protein, human; D047908:Intracellular Signaling Peptides and Proteins; D060588:LIM Domain Proteins; C085117:LMO2 protein, human; D008667:Metalloproteins; D008840:Microfilament Proteins; D009363:Neoplasm Proteins; D011518:Proto-Oncogene Proteins; D015260:Neprilysin