title: Influence of protein hydrolysis on the growth kinetics of β-lg fibrils.
abstract: Recently it was found that protein hydrolysis is an important step in the formation of β-lactoglobulin fibrils at pH 2 and elevated temperatures. The objective of the present study was to further investigate the influence of hydrolysis on the kinetics of fibril formation. Both the hydrolysis of β-lactoglobulin and the growth of the fibrils were followed as a function of time and temperature, using SDS polyacrylamide gel electrophoresis and a Thioflavin T fluorescence assay. As an essential extension to existing models, the quantification of the effect of the hydrolysis on the fibrillar growth was established by a simple polymerization model including a hydrolysis step.
authors.affiliation: Physics and Physical Chemistry of Foods, Department of Agrotechnology and Food Sciences, Wageningen University, Bomenweg 2, 6703 HD, The Netherlands. ardy.nijboer@wur.nl
authors.forename: Ardy
authors.initials: A
authors.lastname: Kroes-Nijboer
authors.forename: Paul
authors.initials: P
authors.lastname: Venema
authors.forename: Jacob
authors.initials: J
authors.lastname: Bouman
authors.forename: Erik
authors.initials: E
authors.lastname: van der Linden
mesh_terms: D052160:Benzothiazoles; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D007782:Lactoglobulins / Q000737:chemistry* / Q000378:metabolism*; D008956:Models, Chemical; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D013696:Temperature; D013844:Thiazoles / Q000378:metabolism
chemical_list: D052160:Benzothiazoles; D007782:Lactoglobulins; D010446:Peptide Fragments; D013844:Thiazoles; C009462:thioflavin T