title: An in vivo engineered nerve conduit--fabrication and experimental study in rats.
abstract: BACKGROUND
  Several types of nerve conduits have been used for peripheral nerve gap bridging. This study investigated the in vivo engineering of a biological nerve conduit and its suitability for nerve gap bridging.
  
  
  METHODS
  A 19-mm long polyvinyl chloride (PVC) tube was implanted parallely to the sciatic nerve. After implantation, a connective tissue cover developed around the PVC-tube, the so-called biogenic conduit. Histological cross-sections were performed after 1, 2, 3, and 4 weeks. Wall thicknesses were measured and all vessels per cross-section were counted. The biogenic conduit filled with fibrin was used to bridge a 15-mm long nerve gap in the sciatic lesion model of the rat (n = 8). The results of nerve repair with the conduit were compared to the autologous nerve graft (n = 8). Sciatic functional index (SFI), nerve area, axon count, myelination index, and ratio of total myelinated fiber area/nerve area (N-ratio) were analyzed after 4 weeks.
  
  
  RESULTS
  The wall thickness of biogenic conduits increased over the 4 weeks implantation time. Biogenic conduits revealed highest number of vessels per cross-section after 4 weeks. The results of SFI analysis did not show significant difference between the repairs with biogenic conduit and autologous nerve graft. Nerve area and axon count in the biogenic conduit group were significantly lower than in the autologous nerve group (P < 0.001). The biogenic conduit group showed significant higher myelination values, but lower N-ratio when compared to the nerve graft group (P < 0.001).
  
  
  CONCLUSIONS
  The in vivo engineered conduits allow nerve gap bridging of 15 mm. However, quality of regeneration after 4 weeks observation time is not comparable to autologous nerve grafts. Whether biogenic conduits might be a suitable alternative to artificial and biological conduits for gap bridging will have to be evaluated in further studies.
authors.affiliation: Department of Plastic and Hand Surgery, Albert-Ludwigs University, Freiburg, Germany. vincenzo.penna@uniklinik-freiburg.de
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Penna
authors.forename: Beatrix
authors.initials: B
authors.lastname: Munder
authors.forename: G-Bjoern
authors.initials: GB
authors.lastname: Stark
authors.forename: Eva M
authors.initials: EM
authors.lastname: Lang
mesh_terms: D000818:Animals; D057005:Bioengineering; D004311:Double-Blind Method; D005260:Female; D008866:Microsurgery / Q000295:instrumentation / Q000379:methods*; D009416:Nerve Regeneration; D009431:Neural Conduction; D051381:Rats; D019651:Plastic Surgery Procedures / Q000379:methods; D020127:Recovery of Function; D012584:Sciatic Nerve / Q000033:anatomy & histology / Q000293:injuries / Q000502:physiology / Q000601:surgery*