title: Fractionated irradiation of five human lung cancer cell lines and prediction of survival according to a radiobiology model.
abstract: BACKGROUND
  This study evaluates a predictive radiobiology model by measurements of surviving fraction (SF) by the clonogenic assay or the extrapolation method and the proliferation rate in vitro. It is hypothesized that incorporating proliferation to intrinsic radiosensitivity, measured by SF, to predict radiation responsiveness after fractionated irradiation adds to the model's accuracy. Materials and Methods. Five lung cancer cell lines with known SF after 1 Gy (SF1), and also SF2 and SF5, were irradiated with three different fractionation regimes; 10 × 1 Gy, 5 × 2 Gy or 2 × 5 Gy during the same total time to achieve empirical SF. In addition, the SF1, SF2 and SF5 after fractionated irradiation was calculated for each cell line based on the already known single fraction SF and with or without a proliferation factor. The results were compared to the empirical data.
  
  
  CONCLUSIONS
  By using the clonogenic assay to measure radiosensitivity, prediction of radiosensitivity was improved after fractionated radiotherapy when proliferation was used in the radiobiology model. However, this was not the case in the cell lines where the extrapolation method was used to calculate SF. Thus, a radiobiology model including intrinsic radiosensitivity, measured by the clonogenic assay, as well as proliferation, is better at predicting survival after fractionated radiotherapy, compared to the use of intrinsic radiosensitivity alone.
authors.affiliation: Department of Oncology-Pathology, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden. mattias.hedman@ki.se
authors.forename: Mattias
authors.initials: M
authors.lastname: Hedman
authors.forename: Michael
authors.initials: M
authors.lastname: Bergqvist
authors.forename: Daniel
authors.initials: D
authors.lastname: Brattström
authors.forename: Ola
authors.initials: O
authors.lastname: Brodin
mesh_terms: D018287:Carcinoma, Large Cell / Q000473:pathology / Q000532:radiotherapy; D002294:Carcinoma, Squamous Cell / Q000473:pathology / Q000532:radiotherapy; D002470:Cell Survival / Q000528:radiation effects*; D019583:Dose Fractionation, Radiation; D005720:Gamma Rays; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology / Q000532:radiotherapy*; D011836:Radiation Tolerance; D011878:Radiotherapy; D011879:Radiotherapy Dosage; D055752:Small Cell Lung Carcinoma / Q000473:pathology / Q000532:radiotherapy; D014407:Tumor Cells, Cultured; D014410:Tumor Stem Cell Assay