title: [Effects of verapamil on the proliferation, DNA synthesis and migration of human hepatoma cell line HepG2].
abstract: OBJECTIVE
  To study the effects of verapamil (VR) on proliferation, DNA synthesis and migration of human hepatoma cell line HepG2.
  
  
  METHODS
  HepG2 cells were treated with different concentration of VR (0,1, 5, 10, 15, 20 microg/mL) for different time point. Cell proliferation was evaluated by MTT assay and DNA synthesis was determined by 5-ethynyl-2'-deoxyuridine (Edu) fluorescent assay. The migration of cells was measured by Scratch wound migration assay.
  
  
  RESULTS
  MTT assay demonstrated that the different concentration of VR (1, 5, 10, 15, 20 microg/mL) could inhibit the proliferation of HepG2 cells after 24 h and 48 h treatment (P < 0.05) except 1, 5 microg/mL group processing for 24 h (P > 0.05). Edu fluorescent assay showed that, the proportion of HepG2 cells in the stage of DNA synthesis was (51.5 +/- 3.78)%, (52.4 +/- 3.26)%, (53.1 +/- 1.94)%, (39.6 +/- 4.25)%, (40.2 +/- 2.67)%, (42.6 +/- 3.13)% respectively for the treatment of 0, 1, 5, 10, 15, 20 microg/mL concentration of VR for 24 h. DNA synthesis in the cells treated with VR (10, 15 and 20 microg/mL concentration) was lower than that in the cells treated with 0, 1, 5 microg/mL concentrations of VR (P < 0.05). Scratch wound migration assay indicated that treatment with VR (10, 15 and 20 microg/mL) for 24 h significantly inhibited cells migration distance (P < 0.05).
  
  
  CONCLUSIONS
  Calcium channel blocker VR could inhibit the cell proliferation and migration of human hepatoma cell line HepG2.
authors.affiliation: Department of Gastroenterology, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Ning-lin
authors.initials: NL
authors.lastname: Liang
authors.forename: Yan
authors.initials: Y
authors.lastname: Wei
authors.forename: Wen-chao
authors.initials: WC
authors.lastname: Wu
authors.forename: Xiao-jing
authors.initials: XJ
authors.lastname: Liu
authors.forename: Li
authors.initials: L
authors.lastname: Yang
mesh_terms: D002121:Calcium Channel Blockers / Q000494:pharmacology; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D004247:DNA / Q000096:biosynthesis*; D056945:Hep G2 Cells; D006801:Humans; D014700:Verapamil / Q000494:pharmacology*
chemical_list: D002121:Calcium Channel Blockers; D004247:DNA; D014700:Verapamil