title: Collateral vessel growth induced by femoral artery ligature is impaired by denervation.
abstract: Innervation plays an important role in development and remodeling of blood vessels. However, very little is known whether innervation is involved in arteriogenesis. In the present study, we tested the hypothesis that innervation may contribute to the process of arteriogenesis induced by ligature of femoral artery in rat/rabbit hind limb with or without denervation. We found that: (1) angiography showed more collateral vessels in the ligature side than that in ligature plus denervation side; (2) collateral vessels in denervation side was characterized by an inward remodeling; (3) in both collateral vessels (CVs) from only femoral ligature side as well as the ligature plus denervation side, ICAM-1 and VCAM-1 expression was up-regulated but increased VCAM-1 was more evident in the adventitia of collateral vessels of only femoral ligature side; (4) 7 days after surgery, in CVs from the femoral ligature side only, numerous macrophages (RAM11 positive cells) and high cell proliferation ratio (ki67 positive cells) were detected, but they were less in the denervation side. In conclusion, our data demonstrate for the first time that neural regulation is one of the factors that contributes to collateral vessel growth in rat/rabbit hind limb ischemic model by showing collateral vessel growth induced by femoral artery ligature is impaired by denervation.
authors.affiliation: Department of Histology & Embryology, Xiangya School of Medicine, Central South University, 172 Tong-Zhi-Po Road, Changsha, 410078 Hunan, People's Republic of China.
authors.forename: Ming-Ying
authors.initials: MY
authors.lastname: Luo
authors.forename: Bao-Lin
authors.initials: BL
authors.lastname: Yang
authors.forename: Feng
authors.initials: F
authors.lastname: Ye
authors.forename: Xiaoqiong
authors.initials: X
authors.lastname: Wu
authors.forename: Song
authors.initials: S
authors.lastname: Peng
authors.forename: Bin
authors.initials: B
authors.lastname: Yi
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Wu
authors.initials: W
authors.lastname: Zhu
authors.forename: Hua
authors.initials: H
authors.lastname: Luo
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Wen
authors.forename: Wei-Jun
authors.initials: WJ
authors.lastname: Cai
authors.forename: Jutta
authors.initials: J
authors.lastname: Schaper
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Schaper
mesh_terms: D000792:Angiography; D000818:Animals; D049109:Cell Proliferation; D003097:Collateral Circulation / Q000502:physiology*; D003714:Denervation; D005263:Femoral Artery / Q000601:surgery*; D006614:Hindlimb / Q000033:anatomy & histology / Q000098:blood supply* / Q000294:innervation; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D019394:Ki-67 Antigen / Q000378:metabolism; D008026:Ligation; D008264:Macrophages / Q000378:metabolism; D018919:Neovascularization, Physiologic; D016900:Neurofilament Proteins / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000601:surgery*; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism
chemical_list: D019394:Ki-67 Antigen; D016900:Neurofilament Proteins; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1