title: Real-time aptamer quantum dot fluorescent flow sensor.
abstract: The goal of this work was to develop and test a novel real-time biosensing approach which can be adapted to either environmental or clinical monitoring of biological pathogens. We have developed a working prototype of a real-time aptamer-based fluorescent flow sensor. The sensor utilizes a competitive displacement approach to measure the binding of the analyte, which keeps the nonspecific binding below detectable levels. The complex of surface-immobilized DNA aptamer with fluorescent complementary oligonucleotide releases the oligonucleotide upon binding with a specific target, which is translated by a decrease in fluorescence. Bright and stable fluorescence of quantum dots is utilized for prolonged detection of the analyte in flow conditions. The real-time sensor prototype is developed with previously characterized ATP-specific aptamer and is capable of specifically detecting 0.1 mM of ATP in biological buffer, with a quantitative response up to 5 mM. The developed prototype is portable and easy to use and its design allows further miniaturization and multiplexing. The developed real-time sensing approach can be adapted to a variety of targets of environmental and clinical significance.
authors.affiliation: Smart Polymers Research Corporation, 108 4th Street, Belleair Beach, FL 33786, USA.
authors.forename: Anastasia
authors.initials: A
authors.lastname: Bogomolova
authors.forename: Matt
authors.initials: M
authors.lastname: Aldissi
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis; D052157:Aptamers, Nucleotide; D015374:Biosensing Techniques / Q000379:methods*; D005453:Fluorescence; D045663:Quantum Dots
chemical_list: D052157:Aptamers, Nucleotide; D000255:Adenosine Triphosphate