title: A sensitive monoclonal antibody-based enzyme-linked immunosorbent assay for chlorpyrifos residue determination in Chinese agricultural samples.
abstract: A monoclonal antibody-based competitive antibody-coated enzyme-linked immunosorbent assay (ELISA) was developed and optimized for determining chlorpyrifos residue in agricultural products. The IC(50) and IC(10) of this ELISA were 3.3 ng/mL and 0.1 ng/mL respectively. The average recoveries in six agricultural products were between 79.5% and 118.0%, with the intra-assay coefficient of variation being less than 8 %. The limit of detection for all tested products was 30 ng/g. To the best of our knowledge, this assay has the best specificity among all the published research on ELISAs for chlorpyrifos.
authors.affiliation: Institute of Pesticide and Environmental Toxicology, Zhejiang University, Hangzhou, China.
authors.forename: Yi H
authors.initials: YH
authors.lastname: Liu
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.forename: Yi R
authors.initials: YR
authors.lastname: Guo
authors.forename: Chun M
authors.initials: CM
authors.lastname: Wang
authors.forename: Xiao
authors.initials: X
authors.lastname: Liang
authors.forename: Guo N
authors.initials: GN
authors.lastname: Zhu
mesh_terms: D000911:Antibodies, Monoclonal; D004390:Chlorpyrifos / Q000032:analysis* / Q000737:chemistry; D002849:Chromatography, Gas; D018556:Crops, Agricultural / Q000737:chemistry*; D003429:Cross Reactions; D004784:Environmental Monitoring / Q000295:instrumentation / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation / Q000379:methods*; D020128:Inhibitory Concentration 50; D007306:Insecticides / Q000032:analysis* / Q000737:chemistry; D010573:Pesticide Residues / Q000032:analysis / Q000737:chemistry
chemical_list: D000911:Antibodies, Monoclonal; D007306:Insecticides; D010573:Pesticide Residues; D004390:Chlorpyrifos