title: Inductive properties of polypyridyl ruthenium complexes significantly regulate various protein distributions in Escherichia coli.
abstract: Ruthenium complexes with similar octahedral structures but different intrinsic inductive properties significantly influence the total cellular protein distributions, which may affect different metabolic pathways. A systematic study of the relationship between ruthenium complexes and Escherichia coli was undertaken, using two-dimensional gel electrophoresis analysis and the identification of various proteins by mass data mining. Based on the low similarities (<40%) between the total protein distributions, the inductive properties of the ruthenium complexes are relevant to the formation of the protein-Ru interaction in addition to the Ru-DNA interaction. Two major protein functions in E. coli BL21 that were reduced by compound 1 were oxidoreductases and transporters, corresponding to 29% and 25% of the 24 down-regulated proteins. The main biological processes of the proteins down-regulated by compound 1 were related to carbohydrate reactions, including in transport, tricarboxylic acid (TCA) cycle, glycolysis, and gluconeogenesis. All four ruthenium complexes shared similar up-regulated proteins, including clpB and kpyk1, and down-regulated similar proteins, including ompA and ybbN. This result supports that the presence of Ru-protein interactions is a major factor affecting bacteria growth, and particularly transport and carbohydrate-related reactions.
authors.affiliation: Genomics Research Center, Academia Sinica, Taipei 115, Taiwan, ROC.
authors.forename: Ming-Yi
authors.initials: MY
authors.lastname: Ho
authors.forename: Min-Lun
authors.initials: ML
authors.lastname: Chiou
authors.forename: Wan-Shan
authors.initials: WS
authors.lastname: Du
authors.forename: Fang Ying
authors.initials: FY
authors.lastname: Chang
authors.forename: Yau-Hung
authors.initials: YH
authors.lastname: Chen
authors.forename: Yu-Jong
authors.initials: YJ
authors.lastname: Weng
authors.forename: Chien-Chung
authors.initials: CC
authors.lastname: Cheng
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D056831:Coordination Complexes / Q000737:chemistry*; D049071:Endopeptidase Clp; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006360:Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D018832:Molecular Chaperones / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D050862:Oxidoreductases Acting on Sulfur Group Donors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012428:Ruthenium / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014233:Tricarboxylic Acids / Q000737:chemistry / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D001425:Bacterial Outer Membrane Proteins; D056831:Coordination Complexes; D029968:Escherichia coli Proteins; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D014233:Tricarboxylic Acids; C078167:OMPA outer membrane proteins; D012428:Ruthenium; D050862:Oxidoreductases Acting on Sulfur Group Donors; C510399:YbbN protein, E coli; D049071:Endopeptidase Clp; C069775:ClpB protein, E coli