title: High-efficiency cell seeding method by relatively hydrophobic culture strategy.
abstract: Cell adhesion efficiency is one of the key factors affecting the results of manufacturing tissue engineering constructs. High efficiency is required for seeding low proliferation cells onto scaffolds. In this study, we designed a strategy to improve the efficiency of cell adhesion using hydrophobic cell culture environment to enhance cells adhering to a scaffold. Cells have lower affinity to the surface of polydimethylsiloxane (PDMS) than tissue culture polystyrene (TCPS) plates. When cells were cultured with gelatin microspheres or chitosan films in a PDMS-coated plate instead of a normal TCPS plate, there was a significant increase in cell attachment efficiency. Cells cultured in the PDMS-coated system tended to selectively attach onto the gelatin microspheres or chitosan films, which are relatively more hydrophilic than the PDMS surface. However, minimal cell attachment on gelatin microspheres or chitosan films was observed when gelatin microspheres or chitosan films were placed in normal TCPS plate. Cell counting experiments with gelatin microspheres in the PDMS-coated system resulted in a cell attachment efficiency of 89.8% after 1 day of cultivation, whereas the cell attachment efficiency was less than 1% in normal TCPS plate. The results demonstrate that the method is easy to use and could be useful for fast cultivation of cell-scaffold constructs.
authors.affiliation: Institute of Biomedical Engineering, College of Engineering, College of Medicine, National Taiwan University, Taipei 100, Taiwan.
authors.forename: Chin-Hsiung
authors.initials: CH
authors.lastname: Hsieh
authors.forename: Wei-Ti
authors.initials: WT
authors.lastname: Kuo
authors.forename: Yi-Cheng
authors.initials: YC
authors.lastname: Huang
authors.forename: Yi-You
authors.initials: YY
authors.lastname: Huang
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D048271:Chitosan; D004129:Dimethylpolysiloxanes; D005780:Gelatin; D057927:Hydrophobic and Hydrophilic Interactions; D051379:Mice; D008863:Microspheres; D041681:NIH 3T3 Cells; D054457:Tissue Scaffolds
chemical_list: D004129:Dimethylpolysiloxanes; C013830:baysilon; D005780:Gelatin; D048271:Chitosan