title: Rapid HCP5 single-nucleotide polymorphism genotyping: a simple allele-specific PCR method for prediction of hypersensitivity reaction to Abacavir.
abstract: BACKGROUND
  The most important factor limiting the success of an antiretroviral therapy is toxicity. The HLA-B*5701 allele is predictive of hypersensitivity reaction to Abacavir, and this gene is in a perfect linkage disequilibrium with the rs2395029 SNP present in the HCP5 gene.
  
  
  METHODS
  Genomic DNA was extracted from blood obtained from 201 unrelated healthy Argentinean volunteers. The DNA was subjected to an allele-specific PCR method. Sequencing was performed to validate the test results.
  
  
  RESULTS
  We were successful to amplify specific fragment of interest from the DNA samples. The method is easy, specific and reproducible.
  
  
  CONCLUSIONS
  The application of this methodology is a rapid and simple method to detect the HCP5 polymorphism (rs2395029) previous to administration of Abacavir in patients with HIV infection.
authors.affiliation: Laboratorio de Análisis Clínicos Especializados (LACE), Córdoba, Argentina.
authors.forename: Cristian A
authors.initials: CA
authors.lastname: Galván
authors.forename: Osvaldo C
authors.initials: OC
authors.lastname: Elbarcha
authors.forename: Eduardo J
authors.initials: EJ
authors.lastname: Fernández
authors.forename: Dante M
authors.initials: DM
authors.lastname: Beltramo
authors.forename: Néstor Walter
authors.initials: NW
authors.lastname: Soria
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000483:Alleles; D019380:Anti-HIV Agents / Q000009:adverse effects*; D015224:Dideoxynucleosides / Q000009:adverse effects*; D004342:Drug Hypersensitivity / Q000097:blood / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D008285:Major Histocompatibility Complex / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011237:Predictive Value of Tests; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated; D012016:Reference Values; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D055815:Young Adult; D000099227:Abacavir
chemical_list: D019380:Anti-HIV Agents; D015224:Dideoxynucleosides; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated; D000099227:Abacavir; C080521:HCP5 long noncoding RNA, human