title: Development of an animal model for endometrial ablation using trichloroacetic acid.
abstract: OBJECTIVE
  To develop an animal model of endometrial ablation, and to evaluate the histologic effects of trichloroacetic acid (TCA) in the uterine cavity.
  
  
  METHODS
  Experimental prospective.
  
  
  METHODS
  Department of gynecology.
  
  
  METHODS
  Thirty female adult rats.
  
  
  METHODS
  Animals were submitted to injection of TCA in one uterine horn and saline solution in the other. Group 1 was sacrificed the day after the procedure. Group 2 was sacrificed in phase of diestrus. Superficial epithelia of the endometrium, stromal thickness, endometrial glands, and myometrium thickness were compared among the uterine horns of the same rats of group 1. The same evaluation was performed in group 2. Endometrial regeneration was evaluated.
  
  
  METHODS
  Histologic effects.
  
  
  RESULTS
  In group 1, histologic parameters showed endometrial destruction on TCA injected uterine horn. In group 2, four rats died after the procedure, and six rats had no viable material. In the rest of the group, TCA-injected uterine horns showed endometrial destruction. Superficial epithelia of the endometrium and stromal thickness were similar between TCA uterine horn from groups. However, the number of endometrial glands was higher in group 1.
  
  
  CONCLUSIONS
  The study developed an experimental model for endometrial ablation. TCA acid is a potent agent for endometrial ablation in rat model. No endometrial regeneration was observed after recovery of cycle.
authors.affiliation: Department of Gynecology, University of Sao Paulo, Sao Paulo, Brazil. mcocuzza@uol.com.br
authors.forename: Mariana Amora
authors.initials: MA
authors.lastname: Cocuzza
authors.forename: Marcello
authors.initials: M
authors.lastname: Cocuzza
authors.forename: Gustavo A R
authors.initials: GA
authors.lastname: Maciel
authors.forename: Eduardo Vieira
authors.initials: EV
authors.lastname: da Motta
authors.forename: Ana P T
authors.initials: AP
authors.lastname: Cardoso
authors.forename: Jose M
authors.initials: JM
authors.lastname: Soares
authors.forename: Edmund C
authors.initials: EC
authors.lastname: Baracat
mesh_terms: D000818:Animals; D002424:Caustics / Q000008:administration & dosage / Q000494:pharmacology*; D004717:Endometrium / Q000187:drug effects* / Q000473:pathology; D030762:Estrous Cycle; D005260:Female; D007267:Injections; D023421:Models, Animal; D051381:Rats; D012038:Regeneration / Q000187:drug effects; D013997:Time Factors; D014238:Trichloroacetic Acid / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D002424:Caustics; D014238:Trichloroacetic Acid