title: Arabidopsis cryptochrome 1 interacts with SPA1 to suppress COP1 activity in response to blue light.
abstract: Plant photoreceptors mediate light suppression of the E3 ubiquitin ligase COP1 (CONSTITUTIVE PHOTOMORPHOGENIC 1) to affect gene expression and photomorphogenesis. However, how photoreceptors mediate light regulation of COP1 activity remains unknown. We report here that Arabidopsis blue-light receptor cryptochrome 1 (CRY1) undergoes blue-light-dependent interaction with the COP1-interacting protein SPA1 (SUPPRESSOR OF PHYTOCHROME A). We further show that the CRY1-SPA1 interaction suppresses the SPA1-COP1 interaction and COP1-dependent degradation of the transcription factor HY5. These results are consistent with a hypothesis that photoexcited CRY1 interacts with SPA1 to modulate COP1 activity and plant development.
authors.affiliation: Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.forename: Zecheng
authors.initials: Z
authors.lastname: Zuo
authors.forename: Hongtao
authors.initials: H
authors.lastname: Liu
authors.forename: Xuanming
authors.initials: X
authors.lastname: Liu
authors.forename: Chentao
authors.initials: C
authors.lastname: Lin
mesh_terms: D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D050976:Basic-Leucine Zipper Transcription Factors / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000378:metabolism*; D056931:Cryptochromes / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D008027:Light; D009687:Nuclear Proteins / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000378:metabolism; D036226:Seedlings / Q000254:growth & development / Q000378:metabolism; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D050976:Basic-Leucine Zipper Transcription Factors; C538962:CRY1 protein, Arabidopsis; D018797:Cell Cycle Proteins; D056931:Cryptochromes; C109254:HY5 protein, Arabidopsis; D009687:Nuclear Proteins; C118571:SPA1 protein, Arabidopsis; C471983:AT2G32950 protein, Arabidopsis; D044767:Ubiquitin-Protein Ligases