title: Conversion of airborne nerolidol to DMNT emission requires additional signals in Achyranthes bidentata.
abstract: DMNT biosynthesis was proposed to proceed via (E)-nerolidol in plants a decade ago. However, (E)-nerolidol function as airborne signal/substrate for in-vivo biosynthesis of DMNT remains to be investigated and the regulation of DMNT production and emission is largely unknown. We address both of these aspects using Achyranthes bidentata model plant in conjunction with deuterium-labeled d(5)-(E)-nerolidol, headspace, GC-FID, and GC/MS-based absolute quantification approaches. We demonstrate that airborne (E)-nerolidol is specifically metabolized in-vivo into DMNT emission, but requires airborne VOC MeJA or predator herbivore as additional environmental signal. In addition, we provide new insight into the complex regulation underlying DMNT emission, and highlight the importance of studying multiple environmental factors on emission patterns of plant VOCs and their mechanistic regulation.
authors.affiliation: Laboratory of Biologically Active Compounds, Department of Biological Production, Akita Prefectural University, Akita, Japan. tamo_chem@akita-pu.ac.jp
authors.forename: Shigeru
authors.initials: S
authors.lastname: Tamogami
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Makoto
authors.initials: M
authors.lastname: Abe
authors.forename: Koji
authors.initials: K
authors.lastname: Noge
authors.forename: Randeep
authors.initials: R
authors.lastname: Rakwal
authors.forename: Ganesh Kumar
authors.initials: GK
authors.lastname: Agrawal
mesh_terms: D029044:Achyranthes / Q000378:metabolism*; D000475:Alkenes / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D012717:Sesquiterpenes / Q000378:metabolism*; D015398:Signal Transduction; D055549:Volatile Organic Compounds / Q000378:metabolism; D013729:Terpenes
chemical_list: D000475:Alkenes; D012717:Sesquiterpenes; D055549:Volatile Organic Compounds; C411254:4,8-dimethyl-1,3,7-nonatriene; D013729:Terpenes; C037055:nerolidol