title: [Protective effects of lidocaine on hyperoxia-exposed type Ⅱalveolar epithelial cells from premature rats].
abstract: OBJECTIVE
  To investigate the effects of lidocaine on apoptosis and proliferation of hyperoxia-exposed type Ⅱalveolar epithelial cells (AECⅡ) from premature rats.
  
  
  METHODS
  Primary cultured AEC Ⅱ isolated from premature rats were randomly divided into four groups: air, air+ lidocaine (20 μg/mL), 95%O2/5%CO2, and 95%O2/5%CO2+ lidocaine. The cells in each group were collected 24 hrs after culture in ordinary incubators (37℃,5%CO2). The proliferation and apoptosis of AEC Ⅱ were detected by flow cytometry. Protein levels of proliferating cell nuclear antigen (PCNA) were measured by Western blot.
  
  
  RESULTS
  The apoptosis rate of AECⅡincreased, the percentages of G2/M and S phase cells decreased and the protein levels of PCNA decreased significantly in the group exposed to 95%O2/5%CO2 compared with the group exposed to air (P<0.01). Lidocaine treatment decreased apoptosis rate of AECⅡ, increased percentage of G2/M and S phase cells, and increased protein levels of PCNA.
  
  
  CONCLUSIONS
  Hyperoxia can increase apoptosis and inhibit proliferation of AECⅡ in premature rats. Lidocaine may have protective effects against the AECⅡ injuries.
authors.affiliation: Department of Pediatrics, People's Hospital of Hunan, Changsha 410005, China.
authors.forename: Xiao-Fang
authors.initials: XF
authors.lastname: Ding
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Zhong
authors.forename: Bing
authors.initials: B
authors.lastname: Zhang
authors.forename: Jia
authors.initials: J
authors.lastname: Li
mesh_terms: D000818:Animals; D017209:Apoptosis; D002453:Cell Cycle; D019610:Cytoprotection; D004847:Epithelial Cells / Q000187:drug effects / Q000473:pathology; D005260:Female; D018496:Hyperoxia / Q000473:pathology*; D008012:Lidocaine / Q000494:pharmacology*; D008297:Male; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D011650:Pulmonary Alveoli / Q000187:drug effects* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D018809:Proliferating Cell Nuclear Antigen; D008012:Lidocaine