title: Heat shock protein B1 and its regulator genes are negatively correlated with intramuscular fat content in the longissimus thoracis muscle of Hanwoo (Korean cattle) steers.
abstract: In previous proteomic studies, heat shock protein β 1 (HSPB1) was detected as a candidate protein related to meat quality in cattle. This study sought to determine if its gene expression was associated with intramuscular fat content in the longissimus thoracis muscle of Korean cattle (Hanwoo). Tissue from two groups of 10 steers each, low-marbling (mean intramuscular fat content, 7.4 ± 1.5%) and high-marbling (23.5 ± 2.8%), were used for immunoblotting, real-time PCR, and statistical analyses. HSPB1 expression in both mRNA and protein was shown to be negatively related to intramuscular fat content (P < 0.05). Pathway analysis found two genes, TNF receptor superfamily member 6 (FAS) and angiotensinogen (AGT), that were regulators of the HSPB1 gene. The expression of the two genes showed a negative correlation with intramuscular fat content (P < 0.05). These results suggest that HSPB1, FAS, and AGT may be good candidate genes associated with intramuscular fat content in the longissimus muscle of Korean cattle.
authors.affiliation: National Institute of Animal Science, Rural Development Administration, Suwon, Korea.
authors.forename: Nam-Kuk
authors.initials: NK
authors.lastname: Kim
authors.forename: Dajeong
authors.initials: D
authors.lastname: Lim
authors.forename: Seung-Hwan
authors.initials: SH
authors.lastname: Lee
authors.forename: Yong-Min
authors.initials: YM
authors.lastname: Cho
authors.forename: Eung-Woo
authors.initials: EW
authors.lastname: Park
authors.forename: Chang-Soo
authors.initials: CS
authors.lastname: Lee
authors.forename: Bong-Shig
authors.initials: BS
authors.lastname: Shin
authors.forename: Tae-Hun
authors.initials: TH
authors.lastname: Kim
authors.forename: Duhak
authors.initials: D
authors.lastname: Yoon
mesh_terms: D000273:Adipose Tissue; D000818:Animals; D002417:Cattle; D004041:Dietary Fats / Q000032:analysis; D005809:Genes, Regulator / Q000502:physiology*; D055551:HSP27 Heat-Shock Proteins / Q000032:analysis / Q000235:genetics* / Q000502:physiology*; D008297:Male; D008460:Meat / Q000032:analysis; D018482:Muscle, Skeletal / Q000033:anatomy & histology* / Q000737:chemistry; D012333:RNA, Messenger / Q000032:analysis; D056910:Republic of Korea
chemical_list: D004041:Dietary Fats; D055551:HSP27 Heat-Shock Proteins; D012333:RNA, Messenger