title: In situ sol-gel preparation of porous alumina monoliths for chromatographic separations of adenosine phosphates.
abstract: A method enabling the in situ preparation of porous alumina monoliths within 100 μm i.d. fused silica capillaries has been developed. These monoliths were prepared using the sol-gel process from a mixture consisting of an inorganic aluminum salt, a porogen, an epoxide, and a solvent. We investigated the effects of varying the preparation conditions on the physical characteristics of the monoliths with respect to their potential application in chromatographic separations. The best columns were obtained from a mixture of aluminum chloride hexahydrate, N,N-dimethylformamide, water, ethanol and propylene oxide. Adenosine phosphates were then separated in the optimized column with retention increasing according to number of phosphate functionalities.
authors.affiliation: Department of Physical Sciences, Barry University, 11300 NE 2nd Ave, Miami Shores, FL 33161, USA. ZZajickova@mail.barry.edu
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Zajickova
authors.forename: Emir
authors.initials: E
authors.lastname: Rubi
authors.forename: Frantisek
authors.initials: F
authors.lastname: Svec
mesh_terms: D000227:Adenine Nucleotides / Q000302:isolation & purification*; D000537:Aluminum Oxide / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000295:instrumentation* / Q000379:methods; D008855:Microscopy, Electron, Scanning; D010539:Permeability; D044367:Phase Transition
chemical_list: D000227:Adenine Nucleotides; D000537:Aluminum Oxide