title: J3 regulation of flowering time is mainly contributed by its activity in leaves.
abstract: Multiple genetic pathways coordinately determine the timing of the floral transition from vegetative to reproductive development in response to various endogenous and environmental signals. We have recently reported that a novel flowering promoter, DNAJ HOMOLOG 3 (J3), acts downstream of various floral pathways and mediates the transcriptional regulation of two floral pathway integrators, FLOWRING LOCUS T and SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1, through its interaction with a potent flowering repressor, SHORT VEGETATIVE PHASE. Here we show that J3 expression driven by the promoter of SUCROSE TRANSPORTER 2 significantly rescues the late-flowering phenotype of j3-1 loss-of-function mutants, suggesting that J3 activity in leaves mainly contributes to its role in regulating flowering time. Furthermore, we reveal that endogenous expression of J3 requires the cis-element(s) located within J3 coding regions or introns.
authors.affiliation: Department of Biological Sciences, National University of Singapore, Singapore.
authors.forename: Lisha
authors.initials: L
authors.lastname: Shen
authors.forename: Hao
authors.initials: H
authors.lastname: Yu
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D035264:Flowers / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D007438:Introns; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D026901:Membrane Transport Proteins; D010940:Plant Proteins; C407549:SVP protein, Arabidopsis; D014157:Transcription Factors; C078729:sucrose transport protein, plant