title: Spectral study of interaction between poly(L-lysine)-poly(ethylene glycol)-poly(L-lysine) and nucleic acids.
abstract: Polymer-DNA interactions have attracted considerable interests due to their important application in DNA transfection and cellular drug delivery technologies. In this work, a new detection assay for DNA is proposed with a tri-block copolymer poly(L-lysine)-poly(ethylene glycol)-poly(L-lysine) by resonance light scattering technique with the linear ranges from 0.0656 to 6.56 μg ml⁻¹. The detection limit for DNA is 0.42 ng ml⁻¹. Most coexisting substances do not interfere in the detection. UV-spectra and FTIR-spectra were employed to demonstrate the mechanisms of the interaction that the conformation of the DNA changes because the microenvironment of DNA changes.
authors.affiliation: Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Function Molecules, Hubei University, Wuhan 430062, People's Republic of China.
authors.forename: Yu
authors.initials: Y
authors.lastname: He
authors.forename: Qiujun
authors.initials: Q
authors.lastname: Yan
authors.forename: Gongwu
authors.initials: G
authors.lastname: Song
authors.forename: Juan
authors.initials: J
authors.lastname: Chen
mesh_terms: D000818:Animals; D002138:Calibration; D002417:Cattle; D004247:DNA / Q000737:chemistry / Q000494:pharmacology; D004347:Drug Interactions / Q000502:physiology; D004355:Drug Stability; D018014:Gene Transfer Techniques / Q000592:standards; D006863:Hydrogen-Ion Concentration; D009696:Nucleic Acids / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D009994:Osmolar Concentration; D011092:Polyethylene Glycols / Q000737:chemistry* / Q000494:pharmacology; D011107:Polylysine / Q000031:analogs & derivatives / Q000737:chemistry* / Q000494:pharmacology; D012542:Scattering, Radiation; D013057:Spectrum Analysis / Q000379:methods / Q000592:standards; D013997:Time Factors
chemical_list: D009696:Nucleic Acids; D011107:Polylysine; D011092:Polyethylene Glycols; D004247:DNA