title: The miRNA expression profile of the uveal melanoma.
abstract: The miRNA expression profile was initially established to investigate its corresponding function in human uveal melanoma. The miRNA expression profile in human uveal melanoma was analyzed by a micro chip technique. The hsa-miRNA expression between four uveal melanomas and four normal uveal tissues was compared. Based on the bioinformatic approach, chip data was analyzed to select out differentially expressed candidate hsa-miRNAs. Real-time quantitative PCR (RT-PCR) was used to confirm the candidate hsa-miRNAs expression in all samples. The results of miRNA microarray chips that matched with RT-PCR were considered as the miRNA expression which was significantly different between normal tissue and uveal melanomas. In four uveal melanomas, expressions of miRNA-20a, miRNA-106a, miRNA-17, miRNA-21, and miRNA-34a were significantly up-regulated, while miRNA-145 and miRNA-204 expression were significantly down-regulated. We used miRNA microarray analysis as a fast, efficient technology to study biological information. The differentially expressed miRNAs may be involved in uveal melanoma pathogenesis, and may help promote the diagnosis and treatment for uveal melanoma.
authors.affiliation: Beijing Tongren Eye Center, Beijing Tongren Hospital, Beijing 100730, China.
authors.forename: Chenghsun
authors.initials: C
authors.lastname: Yang
authors.forename: Wenbin
authors.initials: W
authors.lastname: Wei
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008545:Melanoma / Q000175:diagnosis / Q000235:genetics; D035683:MicroRNAs / Q000032:analysis*; D020411:Oligonucleotide Array Sequence Analysis; D014604:Uveal Neoplasms / Q000175:diagnosis / Q000235:genetics; D000098943:Uveal Melanoma
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs