title: Cell apoptosis and proliferation in salivary glands of Sjögren's syndrome.
abstract: BACKGROUND
  Sjögren's syndrome (SS) occurs associated with parotid neoplasm, non-Hodgkin's B-cell lymphoma, which could impair the condition or be life-threatening for patients. The aim of this work was to analyze cell proliferation and apoptosis modifications in acinar, ductal and inflammatory infiltrate in salivary glands (SG) in patients with Sjögren Syndrome, keratoconjunctivitis, or stomatitis sicca or in healthy subjects, to establish parameters that indicate the likelihood of malignancy of the disease in populations at risk.
  
  
  METHODS
  A study was performed with n = 58 histological samples of lower lip SG from patients diagnosed with SS, keratoconjunctivitis, or stomatitis sicca (SICCA) and from healthy subjects (C). Ki67 and caspase-3 immunolabeling were performed.
  
  
  RESULTS
  The most important result was significant differences between the three study groups in Ki67 and caspase-3 markers (P < 0.0001) in infiltrated lymphocytes.
  
  
  CONCLUSIONS
  The results of this work are indicative of a high degree of proliferation (85%) in infiltrated lymphocytes (IL) associated with SS which, according the literature, could be considered a risk. Furthermore, the markers used in this work are widely known and represent a lower cost than others and can be used to determine risk groups within the population of SS patients, enabling their follow-up.
authors.affiliation: Physiology Department, School of Dentistry, Córdoba National University, Córdoba, Argentina. beabusamia@hotmail.com
authors.forename: B
authors.initials: B
authors.lastname: Busamia
authors.forename: M A
authors.initials: MA
authors.lastname: Gonzalez-Moles
authors.forename: I
authors.initials: I
authors.lastname: Ruiz-Avila
authors.forename: M
authors.initials: M
authors.lastname: Brunotto
authors.forename: J A
authors.initials: JA
authors.lastname: Gil-Montoya
authors.forename: M
authors.initials: M
authors.lastname: Bravo
authors.forename: C
authors.initials: C
authors.lastname: Gobbi
authors.forename: A
authors.initials: A
authors.lastname: Finkelberg
mesh_terms: D017209:Apoptosis / Q000502:physiology*; D053148:Caspase 3 / Q000032:analysis; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000473:pathology; D006801:Humans; D007637:Keratoconjunctivitis / Q000473:pathology; D019394:Ki-67 Antigen / Q000032:analysis; D008046:Lip / Q000473:pathology; D008214:Lymphocytes / Q000473:pathology; D012307:Risk Factors; D018987:Salivary Ducts / Q000473:pathology; D012469:Salivary Glands / Q000473:pathology*; D012470:Salivary Glands, Minor / Q000473:pathology; D012793:Sialadenitis / Q000473:pathology; D012859:Sjogren's Syndrome / Q000473:pathology*; D014987:Xerostomia / Q000473:pathology
chemical_list: D019394:Ki-67 Antigen; D053148:Caspase 3