title: [Effects of lentinan on interleukin-1β-induced transdifferentiation of human embryonic lung fibroblasts to myofibroblasts].
abstract: OBJECTIVE
  To study the effects of interleukin-1β (IL-1β) on transdifferentiation of human embryonic lung fibroblasts to myofibroblasts and the effects of lentinan on the transdifferentiation.
  
  
  METHODS
  The human embryonic lung fibroblasts were cultured in vitro, and fibroblasts were treated with different concentrations of IL-1β and lentinan. The proliferation activity of the human embryonic lung fibroblasts was evaluated by the Cell Counting Kit-8 (CCK-8). The expression of α-smooth muscle actin (α-SMA) protein was measured by immunocytochemistry. The levels of fibronectin (FN), typeⅠcollagen (ColⅠ) and α-SMA mRNA were detected by RT-PCR.
  
  
  RESULTS
  Compared with the untreated control group, the absorbance value of cell proliferation, α-SMA protein levels, FN, ColⅠand α-SMA mRNA expression were significantly up-regulated after different concentrations of IL-1β (0.1, 1, 10 ng/mL) treatment for 48 hrs (P<0.01). Lentinan treatment inhibited up-regulation of the cell proliferation activity, α-SMA protein levels, FN, ColⅠand α-SMA mRNA expression induced by IL-1β in a dose-independent manner (P<0.01).
  
  
  CONCLUSIONS
  Lentinan can suppress human embryonic lung fibroblast proliferation, fibroblast-myofibroblast transdifferentiation and extra cellular matrix synthesis induced by IL-1β.
authors.affiliation: Department of Respiratory Disease, Yuying Children's Hospital of Wenzhou Medical Collage, Wenzhou, Zhejiang 325027, China.
authors.forename: Long-Teng
authors.initials: LT
authors.lastname: Jin
authors.forename: He
authors.initials: H
authors.lastname: Zhang
authors.forename: Chang-Chong
authors.initials: CC
authors.lastname: Li
authors.forename: Wei-Xi
authors.initials: WX
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Lin
mesh_terms: D000199:Actins / Q000032:analysis / Q000235:genetics; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D054338:Cell Transdifferentiation; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D005353:Fibronectins / Q000032:analysis / Q000235:genetics; D006801:Humans; D053583:Interleukin-1beta / Q000494:pharmacology*; D007912:Lentinan / Q000494:pharmacology*; D058628:Myofibroblasts / Q000166:cytology*
chemical_list: C541116:ACTA2 protein, human; D000199:Actins; D005353:Fibronectins; D053583:Interleukin-1beta; D007912:Lentinan