title: Preparation and characterization of 1,3-dioleoyl-2-palmitoylglycerol.
abstract: 1,3-Dioleoyl-2-palmitoylglycerol, an important triacylglycerol in infant formulas, was effectively enriched by a two-step process: (a) dry fractionation of leaf lard and (b) enzymatic acidolysis of the fractionated leaf lard. In step a, the 1,3-dioleoyl-2-palmitoylglycerol content was increased from 16.77 to 30.73% after programmed temperature treatment of the leaf lard at 60 °C for 20 min followed by 34 °C for 10 h. In step b, 43.72% of the 1,3-dioleoyl-2-palmitoylglycerol content was obtained at the optimal conditions of enzymatic acidolysis: a substrate molar ratio of 1:4 (the fractionated leaf lard/camellia oil fatty acids), 6% (w/w) of enzyme loading, and 6 h of reaction time at 45 °C. On the basis of gas chromatography determination and "deducting score" principle, a model was properly established for characterizing the quality of triacylglycerols enriched with 1,3-dioleoyl-2-palmitoylglycerol. This approach would be a valuable contribution in structured lipids industries because only gas chromatography determination was involved.
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, People's Republic of China.
authors.forename: Xiao Li
authors.initials: XL
authors.lastname: Qin
authors.forename: Yun Ming
authors.initials: YM
authors.lastname: Wang
authors.forename: Yong Hua
authors.initials: YH
authors.lastname: Wang
authors.forename: Hui Hua
authors.initials: HH
authors.lastname: Huang
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
mesh_terms: D000818:Animals; D028244:Camellia / Q000737:chemistry; D005591:Chemical Fractionation; D004041:Dietary Fats / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D006801:Humans; D007223:Infant; D041943:Infant Formula / Q000737:chemistry; D008049:Lipase / Q000378:metabolism; D013552:Swine; D014280:Triglycerides / Q000096:biosynthesis* / Q000737:chemistry
chemical_list: D004041:Dietary Fats; D005227:Fatty Acids; D014280:Triglycerides; D008049:Lipase; C029310:lard; C095376:1,3-dioleoyl-2-palmitoylglycerol