title: Obtaining antimicrobial peptides by controlled peptic hydrolysis of bovine hemoglobin.
abstract: Under standard conditions, the peptides and specially the active peptides were obtained from either the denatured hemoglobin that all structures are completely modified or either the native hemoglobin where all structures are intact. In these conditions, antibacterial peptides were isolated from a very complex peptidic hydrolysate which contains more than one hundred peptides having various sizes and characteristics, involving a complex purification process. The new hydrolysis conditions were obtained by using 40% methanol, 30% ethanol, 20% propanol or 10% butanol. These conditions, where only the secondary structure of hemoglobin retains intact, were followed in order to enrich the hydrolyzed hemoglobin by active peptides or obtain new antibacterial peptides. In these controlled peptic hydrolysis of hemoglobin, a selective and restrictive hydrolysate contained only 29 peptides was obtained. 26 peptides have an antibacterial activity against Micrococcus luteus, Listeria innocua, and Escherichia coli with MIC from 187.1 to 1 μM. Among these peptides, 13 new antibacterial peptides are obtained only in these new hydrolysis conditions.
authors.affiliation: Laboratoire de Procédés Biologiques, Génie Enzymatique et Microbien, IUT A Polytech'Lille-Lille I, Villeneuve d'Ascq, France.
authors.forename: Estelle Yaba
authors.initials: EY
authors.lastname: Adje
authors.forename: Rafik
authors.initials: R
authors.lastname: Balti
authors.forename: Mostafa
authors.initials: M
authors.lastname: Kouach
authors.forename: Pascal
authors.initials: P
authors.lastname: Dhulster
authors.forename: Didier
authors.initials: D
authors.lastname: Guillochon
authors.forename: Naïma
authors.initials: N
authors.lastname: Nedjar-Arroume
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D002417:Cattle; D006454:Hemoglobins / Q000737:chemistry*; D006868:Hydrolysis; D057927:Hydrophobic and Hydrophilic Interactions; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010434:Pepsin A / Q000378:metabolism
chemical_list: D023181:Antimicrobial Cationic Peptides; D006454:Hemoglobins; D010434:Pepsin A