title: Characteristics of high-density lipoprotein subclasses distribution for subjects with desirable total cholesterol levels.
abstract: BACKGROUND
  To investigate alteration of high density lipoproteins (HDL) subclasses distribution in different total cholesterol (TC) levels, mainly the characteristics of HDL subclasses distribution in desirable TC levels and analyze the related mechanisms.
  
  
  METHODS
  ApoA-I contents of plasma HDL subclasses were determined by 2-dimensional gel electrophoresis coupled with immunodetection. 486 Chinese Adults subjects were assigned to different TC groups according to the third Report of NCEP (ATP-III) guidelines.
  
  
  RESULTS
  The increase in contents of small preβ1-HDL, HDL3c, HDL3b, and HDL3a particles clustered and reduce in HDL2b with increased of TC. The distribution of HDL subclasses have shown abnormality characterized by the lower HDL2b (324.2 mg/L) contents and the higher preβ1-HDL (90.4 mg/L) contents for desirable TC Chinese subjects. Among 176 desirable TC subjects, 58.6% subjects with triglyceride (TG)<2.26 mmol/L, 61.2% subjects with HDL-C≥1.03 mmol/L and 88.6% subjects with low density lipoprotein cholesterol(LDL-C)<3.34 mmol/L, and the profile of HDL subclasses distribution for above these subjects was reasonable.
  
  
  CONCLUSIONS
  The particles size of HDL subclasses shifted towards smaller with increased TC levels. The TC was liner with HDL2b contents and those can be reduced 17 mg/L for 0.5 mmol/L increment in TC levels. The HDL subclasses distribution phenotype was not expectation for Chinese Population with desirable TC levels. Thus, from the HDL subclasses distribution point, when assessing the coronary heart disease(CHD) risk not only rely on the TC levels, but also the concentrations of TG, HDL-C and LDL-C must considered in case the potential risk for desirable TC subjects with other plasma lipids metabolism disorders.
authors.affiliation: Laboratory of Endocrinology and Metabolism, West China Hospital, Sichuan University, Chengdu 610041, Sichuan, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Tian
authors.forename: Shiyin
authors.initials: S
authors.lastname: Long
authors.forename: Mingde
authors.initials: M
authors.lastname: Fu
authors.forename: Yinghui
authors.initials: Y
authors.lastname: Liu
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Xu
authors.forename: Lianqun
authors.initials: L
authors.lastname: Jia
mesh_terms: D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D016632:Apolipoprotein A-I / Q000097:blood; D002784:Cholesterol / Q000097:blood*; D008078:Cholesterol, LDL / Q000097:blood; D005260:Female; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood* / Q000145:classification*; D008297:Male; D008875:Middle Aged; D014280:Triglycerides / Q000097:blood
chemical_list: D016632:Apolipoprotein A-I; D008078:Cholesterol, LDL; D008075:Lipoproteins, HDL; D014280:Triglycerides; D002784:Cholesterol