title: Depletion of cellular poly (A) binding protein prevents protein synthesis and leads to apoptosis in HeLa cells.
abstract: The cytoplasmic poly (A) binding protein (PABP) is important in mRNA translation and stability. In yeast, depletion of PABP leads to translation arrest. Similarly, the PABP gene in Drosophila is important for proper development. It is however uncertain, whether mammalian PABP is essential for mRNA translation. Here we showed the effect of PABP depletion on mRNA metabolism in HeLa cells by using a small interfering RNA. Our results suggest that depletion of PABP prevents protein synthesis and consequently leads to cell death through apoptosis. Interestingly, no detectable effect of PABP depletion on transcription, transport and stability of mRNA was observed.
authors.affiliation: Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada N1G2W1.
authors.forename: Mst
authors.initials: M
authors.lastname: Thangima Zannat
authors.forename: Rumpa B
authors.initials: RB
authors.lastname: Bhattacharjee
authors.forename: Jnanankur
authors.initials: J
authors.lastname: Bag
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D055785:Gene Knockdown Techniques; D006367:HeLa Cells; D006801:Humans; D039122:Poly(A)-Binding Protein I / Q000235:genetics / Q000502:physiology*; D014176:Protein Biosynthesis / Q000235:genetics*; D034622:RNA Interference
chemical_list: D039122:Poly(A)-Binding Protein I