title: An in vitro screening method to evaluate chemicals as potential chemotherapeutants to control Aeromonas hydrophila infection in channel catfish.
abstract: OBJECTIVE
  To develop an in vitro screening method to be used for identifying potential effective chemotherapeutants to control Aeromonas hydrophila infections.
  
  
  RESULTS
  Using catfish gill cells G1B and four chemicals (hydrogen peroxide, sodium chloride, potassium permanganate and D-mannose), the feasibility of using an in vitro screening method to identify potential effective chemotherapeutants was evaluated in this study. In vitro screening results revealed that, at concentration of 100 mg l⁻¹, H₂O₂ was the only chemical tested that was able to completely abolish the attachment and invasion of Aer. hydrophila to catfish gill cells. In vivo virulence studies using live channel catfish through bath immersion confirmed that H₂O₂ was the only chemical tested that was able to significantly (P < 0·001) reduce the mortality (from 90 or 100% to 0 or 20%) caused by Aer. hydrophila infections.
  
  
  CONCLUSIONS
  The in vitro screening method using catfish gill cells G1B could be used to initially identify potential effective chemotherapeutants to control Aer. hydrophila.
  
  
  CONCLUSIONS
  An in vitro screening method using catfish gill cells to identify potential effective chemotherapeutants described here will cut cost in research compared with the method of using live fish to screen lead compounds for fish disease control.
authors.affiliation: Aquatic Animal Health Research Unit, USDA-ARS, Auburn, AL, USA. julia.pridgeon@ars.usda.gov
authors.forename: J W
authors.initials: JW
authors.lastname: Pridgeon
authors.forename: P H
authors.initials: PH
authors.lastname: Klesius
authors.forename: X
authors.initials: X
authors.lastname: Mu
authors.forename: L
authors.initials: L
authors.lastname: Song
mesh_terms: D000818:Animals; D001419:Bacteria / Q000187:drug effects; D001424:Bacterial Infections / Q000188:drug therapy / Q000382:microbiology / Q000662:veterinary*; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D005393:Fish Diseases / Q000188:drug therapy* / Q000382:microbiology*; D005880:Gills / Q000166:cytology / Q000187:drug effects; D007059:Ictaluridae