title: Repair of nerve defect with acellular nerve graft supplemented by bone marrow stromal cells in mice.
abstract: The acellular nerve graft that can provide internal structure and extracellular matrix components of the nerve is an alternative for repair of peripheral nerve defects. However, results of the acellular nerve grafting for nerve repair still remain inconsistent. This study aimed to investigate if supplementing bone marrow mesenchymal stromal cells (MSCs) could improve the results of nerve repair with the acellular nerve graft in a 10-mm sciatic nerve defect model in mice. Eighteen mice were divided into three groups (n = 6 for each group) for nerve repairs with the nerve autograft, the acellular nerve graft, and the acellular nerve graft by supplemented with MSCs (5 × 10(5)) fibrin glue around the graft. The mouse static sciatic index was evaluated by walking-track testing every 2 weeks. The weight preservation of the triceps surae muscles and histomorphometric assessment of triceps surae muscles and repaired nerves were examined at week 8. The results showed that the nerve repair by the nerve autografting obtained the best functional recovery of limb. The nerve repair with the acellular nerve graft supplemented with MSCs achieved better functional recovery and higher axon number than that with the acellular nerve graft alone at week 8 postoperatively. The results indicated that supplementing MSCs might help to improve nerve regeneration and functional recovery in repair of the nerve defect with the acellular nerve graft.
authors.affiliation: Orthopedic Research Institute of Chinese PLA, General Hospital of Chinese PLA, Beijing 100853, People's Republic Of China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Zhao
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.forename: Jiang
authors.initials: J
authors.lastname: Peng
authors.forename: Zhiwu
authors.initials: Z
authors.lastname: Ren
authors.forename: Shengfeng
authors.initials: S
authors.lastname: Zhan
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Bin
authors.initials: B
authors.lastname: Zhao
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhao
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Quanyi
authors.initials: Q
authors.lastname: Guo
authors.forename: Wenjing
authors.initials: W
authors.lastname: Xu
authors.forename: Shibi
authors.initials: S
authors.lastname: Lu
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation / Q000379:methods*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008866:Microsurgery / Q000379:methods*; D009416:Nerve Regeneration; D019651:Plastic Surgery Procedures / Q000379:methods; D020127:Recovery of Function; D012584:Sciatic Nerve / Q000293:injuries / Q000502:physiology / Q000601:surgery* / Q000637:transplantation; D017154:Stromal Cells / Q000637:transplantation; D014182:Transplantation, Autologous