title: The P-selectin gene Pro715 allele and low levels of soluble P-selectin are associated with reduced P2Y12 adenosine diphosphate receptor reactivity in clopidogrel-treated patients.
abstract: OBJECTIVE
  To investigate the interrelation between the P-selectin gene (SELP) Pro715 allele, P2Y12 adenosine diphosphate (ADP) receptor reactivity and levels of soluble P-selectin (sP-selectin) in clopidogrel treated patients.
  
  
  METHODS
  The Pro715 allele within SELP was tested by allele specific PCR, sP-selectin was determined by ELISA, and P2Y12 receptor reactivity was analyzed by the VASP assay in 156 patients after angioplasty and stenting.
  
  
  RESULTS
  Carriers of the SELP Pro715 allele had significantly lower levels of sP-selectin and P2Y12 receptor reactivity, and high on-treatment residual P2Y12 receptor reactivity (HRPR) was significantly less frequent compared to non-carriers (both p<0.05). Further, patients within the lowest quartile of sP-selectin had significantly lower reactivity values and also less often HRPR compared to patients with higher sP-selectin (both p<0.01).
  
  
  CONCLUSIONS
  The SELP Pro715 allele is linked to low levels of sP-selectin, and both are associated with decreased P2Y12 ADP receptor reactivity in patients on clopidogrel therapy.
authors.affiliation: Division of Angiology, Department of Internal Medicine II, Medical University of Vienna, Vienna, Austria. thomas.gremmel@meduniwien.ac.at
authors.forename: Thomas
authors.initials: T
authors.lastname: Gremmel
authors.forename: Christoph W
authors.initials: CW
authors.lastname: Kopp
authors.forename: Sabine
authors.initials: S
authors.lastname: Steiner
authors.forename: Daniela
authors.initials: D
authors.lastname: Seidinger
authors.forename: Cihan
authors.initials: C
authors.lastname: Ay
authors.forename: Renate
authors.initials: R
authors.lastname: Koppensteiner
authors.forename: Christine
authors.initials: C
authors.lastname: Mannhalter
authors.forename: Simon
authors.initials: S
authors.lastname: Panzer
mesh_terms: D000328:Adult; D000483:Alleles; D017130:Angioplasty / Q000379:methods; D000077144:Clopidogrel; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D019007:P-Selectin / Q000097:blood* / Q000235:genetics*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D011110:Polymorphism, Genetic; D011392:Proline / Q000235:genetics; D011446:Prospective Studies; D058921:Purinergic P2Y Receptor Antagonists / Q000494:pharmacology; D058925:Receptors, Purinergic P2Y12 / Q000235:genetics*; D013988:Ticlopidine / Q000031:analogs & derivatives / Q000494:pharmacology
chemical_list: D019007:P-Selectin; C548576:P2RY12 protein, human; D010975:Platelet Aggregation Inhibitors; D058921:Purinergic P2Y Receptor Antagonists; D058925:Receptors, Purinergic P2Y12; D011392:Proline; D000077144:Clopidogrel; D013988:Ticlopidine