title: Microfluidic assay of endothelial cell migration in 3D interpenetrating polymer semi-network HA-Collagen hydrogel.
abstract: Cell migration through the extracellular matrix (ECM) is one of the key features for physiological and pathological processes such as angiogenesis, cancer metastasis, and wound healing. In particular, the quantitative assay of endothelial cell migration under the well-defined three dimensional (3D) microenvironment is important to analyze the angiogenesis mechanism. In this study, we report a microfluidic assay of endothelial cell sprouting and migration into an interpenetrating polymer semi-network HA-Collagen (SIPNs CH) hydrogel as ECM providing an enhanced in vivo mimicking 3D microenvironment to cells. The microfluidic chip could provide a well-controlled gradient of growth factor to cells, whereas the hydrogel could mimic a well-defined 3D microenvironment in vivo. (In addition/Furthermore, the microfluidic chip gives a well-controlled gradient of growth factor to cells) For this reason, three types of hydrogel, composed of semi-interpenetrating networks of collagen and hyaluronic acid were prepared, and firstly we proved the role of the hydrogel in endothelial cell migration. The diffusion property and swelling ratio of the hydrogel were characterized. It modulated the migration of endothelial cells in quantified manner, also being influenced by additional synthesis of Matrix metalloproteinase(MMP)-sensitive remodeling peptides and Arginine-glycine-lycinee (RGD) cell adhesion peptides. We successfully established a novel cell migration platform by changing major determinants such as ECM material under biochemical synthesis and under growth factor gradients in a microfluidic manner.
authors.affiliation: Department of Biomedical Engineering, College of Health Science, Korea University, 1-boneji San, Jeongneung-dong, Seongbuk-gu, Seoul 136-100, Korea.
authors.forename: Gi Seok
authors.initials: GS
authors.lastname: Jeong
authors.forename: Gu Han
authors.initials: GH
authors.lastname: Kwon
authors.forename: Ah Ran
authors.initials: AR
authors.lastname: Kang
authors.forename: Bo Young
authors.initials: BY
authors.lastname: Jung
authors.forename: Yongdoo
authors.initials: Y
authors.lastname: Park
authors.forename: Seok
authors.initials: S
authors.lastname: Chung
authors.forename: Sang-Hoon
authors.initials: SH
authors.lastname: Lee
mesh_terms: D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods; D002465:Cell Movement; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry*; D042783:Endothelial Cells / Q000166:cytology*; D005109:Extracellular Matrix / Q000737:chemistry; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry*; D020782:Matrix Metalloproteinases / Q000096:biosynthesis; D044085:Microfluidics / Q000295:instrumentation* / Q000379:methods; D009842:Oligopeptides / Q000737:chemistry; D023822:Tissue Engineering / Q000379:methods
chemical_list: D020100:Hydrogels; D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid; D006820:Hyaluronic Acid; D003094:Collagen; D020782:Matrix Metalloproteinases