title: cTAGE5 mediates collagen secretion through interaction with TANGO1 at endoplasmic reticulum exit sites.
abstract: Cutaneous T-cell lymphoma-associated antigen 5 (cTAGE5), an originally identified tumor antigen, is overexpressed in various cancer cell lines. The cDNA encodes an integral membrane protein containing two coiled-coil motifs and a proline-rich domain. We show that cTAGE5 specifically localizes to the endoplasmic reticulum (ER) exit sites. In addition, cTAGE5 forms a complex with TANGO1 (MIA3), a previously characterized cargo receptor for collagen VII, by the interaction of their coiled-coil motifs. Of interest, cTAGE5, as well as TANGO1, is capable of interacting with the inner-layer coatomer of COPII Sec23/24 complex through their C-terminal proline-rich domains and required for collagen VII secretion. We propose that cTAGE5 acts as a coreceptor of TANGO1 for collagen VII export from the ER.
authors.affiliation: Department of Physiological Chemistry, Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo 113-0033, Japan.
authors.forename: Kota
authors.initials: K
authors.lastname: Saito
authors.forename: Koh
authors.initials: K
authors.lastname: Yamashiro
authors.forename: Yuki
authors.initials: Y
authors.lastname: Ichikawa
authors.forename: Patrik
authors.initials: P
authors.lastname: Erlmann
authors.forename: Kenji
authors.initials: K
authors.lastname: Kontani
authors.forename: Vivek
authors.initials: V
authors.lastname: Malhotra
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Katada
mesh_terms: D000951:Antigens, Neoplasm / Q000378:metabolism*; D051784:Aryl Hydrocarbon Receptor Nuclear Translocator / Q000378:metabolism*; D022181:COP-Coated Vesicles / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism; D002461:Cell Line, Transformed; D003094:Collagen / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism; D009363:Neoplasm Proteins / Q000378:metabolism*; D011485:Protein Binding; D021381:Protein Transport; D011956:Receptors, Cell Surface / Q000378:metabolism
chemical_list: C497396:ARNT protein, human; D000951:Antigens, Neoplasm; D002352:Carrier Proteins; C109278:MIA2 protein, human; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D011956:Receptors, Cell Surface; C064184:serpin-enzyme complex receptor; D051784:Aryl Hydrocarbon Receptor Nuclear Translocator; D003094:Collagen