title: Two-dimensional gel electrophoresis using immobilized pH gradient strips and FlamingoTM fluorescent gel stain identified non-nuclear proteins possibly related to malignant tumour progression.
abstract: The understanding of tumour progression is one of the most important strategies to conquer tumour. QR-32 is a regressive murine fibrosarcoma cell line, and QRsP-11 is a progressive malignant tumour cell clone derived from QR-32. Ina recently published study a differential display analysis for the cytoplasmic proteins was shown by using two-dimensional gel electrophoresis (2-DE) making full use of isoelectric focusing capillary gels and Coomassie brilliant blue R-250 staining. Furthermore, a differential display analysis of the nuclear proteome for QR-32 and QRsP-11 was performed. The present study shows a non-nuclear proteomic differential display analysis, using 2-DE making full use of immobilized pH gradient strips and Flamingo™ fluorescent gel stain, between QR-32 and QRsP-11 to identify particular proteins which may be involved in malignant progression. In QRsP-11 25 proteins were up-regulated, including hypoxia up-regulated protein 1, and 6 were down-regulated compared with QR-32. These results suggest that the identified non-nuclear proteins showing different expression between QR-32 and QRsP-11 possibly related to malignant tumour progression.
authors.affiliation: Department of Biochemistry, Yamaguchi University Graduate School of Medicine, Yamaguchi, Japan. climates@yamaguchi-u.ac.jp
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kuramitsu
authors.forename: Eiko
authors.initials: E
authors.lastname: Hayashi
authors.forename: Futoshi
authors.initials: F
authors.lastname: Okada
authors.forename: Xiulian
authors.initials: X
authors.lastname: Zhang
authors.forename: Yoshiya
authors.initials: Y
authors.lastname: Ueyama
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Nakamura
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003593:Cytoplasm / Q000378:metabolism*; D018450:Disease Progression; D015180:Electrophoresis, Gel, Two-Dimensional; D005354:Fibrosarcoma / Q000378:metabolism*; D007525:Isoelectric Focusing; D051379:Mice; D008810:Mice, Inbred C57BL; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D018892:Proton-Motive Force; D013194:Staining and Labeling; D053719:Tandem Mass Spectrometry; D014407:Tumor Cells, Cultured
chemical_list: D020543:Proteome