title: Galectin-1-expressing stromal cells constitute a specific niche for pre-BII cell development in mouse bone marrow.
abstract: In the bone marrow (BM), stromal cells constitute a supportive tissue indispensable for the generation of pro-B/pre-BI, pre-BII, and immature B lymphocytes. IL-7-producing stromal cells constitute a cellular niche for pro-B/pre-BI cells, but no specific stromal cell microenvironment was identified for pre-BII cells expressing a functional pre-B cell receptor (pre-BCR). However expression of the pre-BCR represents a crucial checkpoint during B-cell development. We recently demonstrated that the stromal cell derived-galectin1 (GAL1) is a ligand for the pre-BCR, involved in the proliferation and differentiation of normal mouse pre-BII cells. Here we show that nonhematopoietic osteoblasts and reticular cells in the BM express GAL1. We observed that pre-BII cells, unlike the other B-cell subsets, were specifically localized in close contact with GAL1(+) reticular cells. We also determined that IL-7(+) and GAL1(+) cells represent 2 distinct mesenchymal populations with different BM localization. These results demonstrate the existence of a pre-BII specific stromal cell niche and indicate that early B cells move from IL-7(+) to GAL1(+) supportive BM niches during their development.
authors.affiliation: Centre d'Immunologie de Marseille-Luminy, Aix Marseille University, Faculté des Sciences de Luminy, Marseille, France;
authors.forename: Frédéric
authors.initials: F
authors.lastname: Mourcin
authors.forename: Caroline
authors.initials: C
authors.lastname: Breton
authors.forename: Julie
authors.initials: J
authors.lastname: Tellier
authors.forename: Priyanka
authors.initials: P
authors.lastname: Narang
authors.forename: Lionel
authors.initials: L
authors.lastname: Chasson
authors.forename: Audrey
authors.initials: A
authors.lastname: Jorquera
authors.forename: Mark
authors.initials: M
authors.lastname: Coles
authors.forename: Claudine
authors.initials: C
authors.lastname: Schiff
authors.forename: Stéphane J C
authors.initials: SJ
authors.lastname: Mancini
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000378:metabolism / Q000502:physiology; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D002454:Cell Differentiation / Q000276:immunology; D002478:Cells, Cultured; D037483:Galectin 1 / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000235:genetics; D015851:Interleukin-7 / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D054420:Pre-B Cell Receptors / Q000378:metabolism; D054448:Precursor Cells, B-Lymphoid / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D055153:Stem Cell Niche / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism / Q000502:physiology*
chemical_list: D037483:Galectin 1; C508605:IL7 protein, human; D015851:Interleukin-7; D054420:Pre-B Cell Receptors; D049452:Green Fluorescent Proteins