title: Human dental pulp stem cells derived from different cryopreservation methods of human dental pulp tissues of diseased teeth.
abstract: BACKGROUND
  Successful isolation of human dental pulp stem cells (hDPSCs) has been documented at least 120h after tooth extraction. Viable hDPSCs have been isolated chiefly from cryopreserved healthy molar teeth and their undigested dental pulp tissue. Isolation of hDPSCs from diseased but vital teeth after cryopreservation has not been reported. This study aimed to isolate hDPSCs from cryopreserved diseased but vital teeth of various tooth types.
  
  
  METHODS
  Fifty tooth samples were divided into group A (n = 20) - freshly derived dental pulp tissues, group B (n = 20) - liquid nitrogen (liq N(2) )-stored dental pulp tissues and group C (n = 10) - liq N(2) -stored intact teeth.
  
  
  RESULTS
  The success rate for hDPSCs isolation was 100% for groups A and B and only 20% for group C. hDPSCs from all groups demonstrated self-renewal properties and similar multipotent potential characteristics of adipogenic, chondrogenic and osteogenic differentiation. In addition, hDPSCs showed high expression of bone-marrow mesenchymal stem-cell markers (CD29, CD90 and CD105) and very low expression of specific hematopoietic cells markers (CD14, CD34 and CD45).
  
  
  CONCLUSIONS
  Our results indicate that hDPSCs isolated from diseased but vital teeth of various tooth types can be stored in liq N(2) for future usage.
authors.affiliation: Department of Dentistry, College of Dental Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan. k0285@ms22.hinet.net
authors.forename: Yuk-Kwan
authors.initials: YK
authors.lastname: Chen
authors.forename: Anderson Hsien-Cheng
authors.initials: AH
authors.lastname: Huang
authors.forename: Anthony Wing-Sang
authors.initials: AW
authors.lastname: Chan
authors.forename: Tien-Yu
authors.initials: TY
authors.lastname: Shieh
authors.forename: Li-Min
authors.initials: LM
authors.lastname: Lin
mesh_terms: D050156:Adipogenesis / Q000502:physiology; D000293:Adolescent; D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000032:analysis; D018952:Antigens, CD34 / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation / Q000502:physiology; D002469:Cell Separation / Q000379:methods; D002648:Child; D020219:Chondrogenesis / Q000502:physiology; D015331:Cohort Studies; D015925:Cryopreservation / Q000379:methods*; D003782:Dental Pulp / Q000166:cytology*; D000071063:Endoglin; D005260:Female; D006801:Humans; D019012:Integrin beta1 / Q000032:analysis; D017493:Leukocyte Common Antigens / Q000032:analysis; D018950:Lipopolysaccharide Receptors / Q000032:analysis; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology; D008875:Middle Aged; D039902:Multipotent Stem Cells / Q000166:cytology / Q000502:physiology; D010012:Osteogenesis / Q000502:physiology; D011956:Receptors, Cell Surface / Q000032:analysis; D013234:Stem Cells / Q000166:cytology*; D018800:Thy-1 Antigens / Q000032:analysis; D014076:Tooth Diseases / Q000473:pathology*; D055815:Young Adult
chemical_list: D015703:Antigens, CD; D018952:Antigens, CD34; D015415:Biomarkers; C064206:ENG protein, human; D000071063:Endoglin; D019012:Integrin beta1; D018950:Lipopolysaccharide Receptors; D011956:Receptors, Cell Surface; D018800:Thy-1 Antigens; D017493:Leukocyte Common Antigens; C516769:PTPRC protein, human