title: IL-29/IL-28A suppress HSV-1 infection of human NT2-N neurons.
abstract: The newly identified cytokines, IL-28/IL-29 (also termed type III IFNs), are able to inhibit a number of viruses. Here, we examined the antiviral effects of IL-29/IL-28A against herpes simplex virus type 1 (HSV-1) in human NT2-N neurons and CHP212 neuronal cells. Both IL-29 and IL-28A could efficiently inhibit HSV-1 replication in neuronal cells, as evidenced by the reduced expression of HSV-1 DNA and proteins. This inhibitory effect of IL-29 and IL-28A against HSV-1 could be partially blocked by antibody to IL-10Rβ, one of the key receptors for IL-29 and IL-28A. To explore the underlying antiviral mechanisms employed by IL-29/IL-28A, we showed that IL-29/IL-28A could selectively induce the expression of several Toll-like receptors (TLRs) as well as activate TLR-mediated antiviral pathway, including IFN regulatory factor 7, IFN-α, and the key IFN-α stimulated antiviral genes.
authors.affiliation: Department of Pathology & Laboratory Medicine, Temple University School of Medicine, Medical Education Research Building, 1052, Philadelphia, PA 19140, USA.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhou
authors.forename: Jieliang
authors.initials: J
authors.lastname: Li
authors.forename: Xu
authors.initials: X
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Ye
authors.forename: Wei
authors.initials: W
authors.lastname: Hou
authors.forename: Jie
authors.initials: J
authors.lastname: Ho
authors.forename: He
authors.initials: H
authors.lastname: Li
authors.forename: Wenzhe
authors.initials: W
authors.lastname: Ho
mesh_terms: D057134:Antibodies, Neutralizing / Q000276:immunology / Q000494:pharmacology; D045744:Cell Line, Tumor; D004279:DNA, Viral / Q000096:biosynthesis; D006561:Herpes Simplex / Q000276:immunology* / Q000473:pathology / Q000821:virology; D018259:Herpesvirus 1, Human / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry; D050839:Interferon Regulatory Factor-7 / Q000096:biosynthesis; D016898:Interferon-alpha / Q000096:biosynthesis; D007372:Interferons; D007378:Interleukins / Q000096:biosynthesis / Q000276:immunology*; D009474:Neurons / Q000276:immunology* / Q000473:pathology / Q000821:virology; D053701:Receptors, Interleukin-10 / Q000037:antagonists & inhibitors* / Q000276:immunology; D051193:Toll-Like Receptors / Q000276:immunology / Q000378:metabolism; D015854:Up-Regulation; D014779:Virus Replication / Q000187:drug effects / Q000276:immunology; D000095022:Interferon Lambda
chemical_list: D057134:Antibodies, Neutralizing; D004279:DNA, Viral; D050839:Interferon Regulatory Factor-7; D016898:Interferon-alpha; D007372:Interferons; D007378:Interleukins; D053701:Receptors, Interleukin-10; D051193:Toll-Like Receptors; C000624353:interferon-lambda, human; D000095022:Interferon Lambda