title: [Microarray data analysis of genes related with erectile dysfunction in diabetic rats].
abstract: OBJECTIVE
  To investigate the changes of gene expression profiles associated with erectile dysfunction in diabetic rats.
  
  
  METHODS
  Affymetrix Gene Chip arrays from the Gene Expression Omnibus (GEO) were used to examine the alterations in the gene expression profiles between streptozotocin-induced diabetic rats and littermate controls, and the data were analyzed with GeneSifter microarray analysis software.
  
  
  RESULTS
  A total of 661 differentially expressed genes were identified, including 280 up-regulated and 381 down-regulated ones. Among the differentially expressed genes, kruppel-like factor 5 (klf5) was upregulated by 4.01 folds and ceruloplasmin(cp) by 5.14 folds; collagen, type XI, alpha1 was down-regulated by 5.84 folds and collagen, type I, alpha1 by 5.77 folds. The 661 differentially expressed genes involved such functional processes as glycoprotein biosynthesis, collagen fibril organization, angiogenesis in wound healing, triglyceride metabolism, cell proliferation and other important biological processes, and some pathways also involved such as fatty acid metabolism, neurodegenerative disorders, and ECM-receptor interactions.
  
  
  CONCLUSIONS
  Some genes such as klf5, cp, and collagen play important roles in the pathophysiology of diabetes-induced erectile dysfunction. Bioinformatic approaches offer a new means for identifying candidate genes and pathways relevant to the pathophysiology of diabetes-induced erectile dysfunction, highlighting also the potential complexity of this disorder.
authors.affiliation: Division of Urinary Surgery of Huiqiao Department, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors.forename: An-Yang
authors.initials: AY
authors.lastname: Wei
authors.forename: Xin-Gui
authors.initials: XG
authors.lastname: Luo
authors.forename: Yong
authors.initials: Y
authors.lastname: Yang
authors.forename: Shu-Hua
authors.initials: SH
authors.lastname: He
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000818:Animals; D019295:Computational Biology; D003921:Diabetes Mellitus, Experimental / Q000150:complications / Q000235:genetics*; D007172:Erectile Dysfunction / Q000209:etiology / Q000235:genetics*; D015870:Gene Expression; D020869:Gene Expression Profiling; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats