title: [Detection of multiple Ophiocordyceps sinensis mutants in premature stroma of Cordyceps sinensis by MassARRAY SNP MALDI-TOF mass spectrum genotyping].
abstract: OBJECTIVE
  To examine the mutants of Ophiocordyceps sinensis (Os) in the stroma of premature Cordyceps sinensis (Cs).
  
  
  METHODS
  Used MassARRAY single nucleotide polymorphism (SNP) MALDI-TOF mass spectrum genotyping, designed eight SNP extension primers on the basis of the scattered, multiple point mutations of known Os mutants within their internal transcribed spacer (ITS) segments, and examined the Os mutant genotypes relating to the GC-biased Os genotype (gb #AB067721) in premature Cs stroma.
  
  
  RESULTS
  The two AT-biased genotypes and the GC-biased Os were simultaneously detected in premature Cs stroma. SNP genotyping also detected at least two other Os genotypes of unknown sequences.
  
  
  CONCLUSIONS
  Coexistence of the three known Os genotypes indicates the existence of possible transition point mutations within Os genes during germination and early maturation of Cs. Simultaneous detection of at least two unknown genotypes coexisting with those known mutants possibly evidences the transversion mutations within Os genes.
authors.affiliation: Pharmanex Beijing Clinical Pharmacology Center, Beijing 100088, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Gao
authors.forename: Xiao-hong
authors.initials: XH
authors.lastname: Li
authors.forename: Jian-qing
authors.initials: JQ
authors.lastname: Zhao
authors.forename: Ji-hong
authors.initials: JH
authors.lastname: Lu
authors.forename: Jia-shi
authors.initials: JS
authors.lastname: Zhu
mesh_terms: D001483:Base Sequence; D032661:Cordyceps / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D005838:Genotype; D008969:Molecular Sequence Data; D017354:Point Mutation; D016133:Polymerase Chain Reaction / Q000379:methods; D020641:Polymorphism, Single Nucleotide; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D017931:DNA Primers