title: The regulation mode of RIN transcription factor involved in ethylene biosynthesis in tomato fruit.
abstract: BACKGROUND
  LE-RIN encodes the RIN (Ripening Inhibitor) transcription factor belonging to the MADS (Mcm1, Agamous, Deficiens, Srf) box family and regulates tomato ripening. The RIN transcription factor plays an indispensable role in ethylene biosynthesis. In the study, we respectively evaluated gene expressions and enzymatic activities of 1-aminocyclopropane-1-carboxylic acid (ACC) synthase (ACS) and ACC oxidase (ACO) and ethylene production to understand the transcriptional regulation of RIN transcription factor in ethylene biosynthesis.
  
  
  RESULTS
  RIN transcription factor generates strong or weak induction of the expression of LE-ACS2, LE-ACS4 and LE-ACO1 associated with ethylene biosynthesis. Exogenous ethylene could positively affect gene expression of mature green AC tomato fruit compared with the control, including LE-ACS2, LE-ACO1 and LE-ACO4. However, LE-ACS6 expression was negatively affected by RIN transcription factor and high ethylene production. Accordingly, the activities of ACC synthase and ACC oxidase and ethylene production were also changed.
  
  
  CONCLUSIONS
  RIN transcription factor has an impact upon ethylene biosynthesis via regulating transcription of LE-ACS2, LE-ACS4, LE-ACO1 and LE-ACS6. These results will help to further clarify the mechanism of tomato fruit development and ripening, and to maintain quality and prolong shelf life of tomato fruit.
authors.affiliation: College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Li
authors.forename: Benzhong
authors.initials: B
authors.lastname: Zhu
authors.forename: Pengyue
authors.initials: P
authors.lastname: Yang
authors.forename: Daqi
authors.initials: D
authors.lastname: Fu
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhu
authors.forename: Yunbo
authors.initials: Y
authors.lastname: Luo
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000235:genetics / Q000378:metabolism; D005030:Ethylenes / Q000096:biosynthesis* / Q000494:pharmacology; D005638:Fruit / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008190:Lyases / Q000235:genetics / Q000378:metabolism; D018551:Solanum lycopersicum / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D005030:Ethylenes; D010940:Plant Proteins; D014157:Transcription Factors; C036216:ethylene; D000594:Amino Acid Oxidoreductases; C059460:1-aminocyclopropane-1-carboxylic acid oxidase; D008190:Lyases; C021773:1-aminocyclopropanecarboxylate synthase