title: Source determination of nitrous oxide based on nitrogen and oxygen isotope tracing dealing with oxygen exchange.
abstract: Source determination of nitrous oxide (N(2)O) from soils has so far been complicated by methodological constraints: the frequently used (15)N tracer method could not differentiate between pathways related to nitrification, that is, nitrifier nitrification (NN), nitrifier denitrification (ND), and nitrification-coupled denitrification (NCD). To overcome this problem, a dual isotope method using both (15)N and (18)O was proposed. However, O exchange between nitrogen oxides and water has been found to disturb such a method. We here explain in detail a novel dual isotope method that allows to quantify O exchange in denitrification and to differentiate N(2)O production from NN, ND, NCD, and fertilizer denitrification (FD). The method has already been applied to a range of soils with good success. Potential of and scope for further improvement of the method are discussed.
authors.affiliation: Department of Soil Quality, Wageningen University and Research Centre, Wageningen, The Netherlands.
authors.forename: Dorien M
authors.initials: DM
authors.lastname: Kool
authors.forename: Jan Willem
authors.initials: JW
authors.lastname: Van Groenigen
authors.forename: Nicole
authors.initials: N
authors.lastname: Wrage
mesh_terms: D058440:Denitrification; D004784:Environmental Monitoring; D009584:Nitrogen / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D009587:Nitrogen Isotopes / Q000032:analysis* / Q000737:chemistry; D009609:Nitrous Oxide / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D010100:Oxygen / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D010103:Oxygen Isotopes / Q000032:analysis* / Q000737:chemistry; D012987:Soil / Q000737:chemistry*; D014867:Water / Q000032:analysis
chemical_list: D009587:Nitrogen Isotopes; D010103:Oxygen Isotopes; D012987:Soil; D014867:Water; D009609:Nitrous Oxide; D009584:Nitrogen; D010100:Oxygen