title: Early occurrence of apoptosis in lymphoid tissues from chickens infected with strains of Newcastle disease virus of varying virulence.
abstract: Newcastle disease virus (NDV), the causative agent of Newcastle disease, is a prevalent problem in the poultry industry and often the cause of severe economic loss. There are many strains of the virus and these have varying virulence. The most virulent strains cause systemic lesions of lymphoid tissues, with necrosis and severe lymphoid depletion. Less virulent strains do not cause as much necrosis, but may predispose to secondary infection with other pathogens. Apoptosis or programmed cell death, has been demonstrated to play a role in the pathogenesis of other paramyxovirus infections, notably those caused by measles and canine distemper viruses. To investigate the role of apoptosis in lymphoid organs during NDV infection, immunohistochemistry for determination of expression of caspase-3, a marker of imminent apoptosis, was performed on formalin-fixed paraffin wax-embedded tissues (spleen, thymus, caecal tonsils and bursa of Fabricius) from 4-week-old chickens infected with NDV strains of varying virulence 2 days previously. The amount of apoptosis was proportional to the severity of the clinical disease elicited by the strains.
authors.affiliation: Department of Veterinary Pathology, College of Veterinary Medicine, University of Georgia, Athens, GA 30602-7388, USA.
authors.forename: L
authors.initials: L
authors.lastname: Harrison
authors.forename: C
authors.initials: C
authors.lastname: Brown
authors.forename: C
authors.initials: C
authors.lastname: Afonso
authors.forename: J
authors.initials: J
authors.lastname: Zhang
authors.forename: L
authors.initials: L
authors.lastname: Susta
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D017209:Apoptosis; D002060:Bursa of Fabricius / Q000473:pathology / Q000821:virology; D053148:Caspase 3 / Q000032:analysis; D002432:Cecum / Q000473:pathology / Q000821:virology; D002645:Chickens / Q000821:virology*; D003588:Cytopathogenic Effect, Viral; D008221:Lymphoid Tissue / Q000201:enzymology / Q000473:pathology* / Q000821:virology; D009521:Newcastle Disease / Q000473:pathology* / Q000821:virology; D009522:Newcastle disease virus / Q000145:classification / Q000276:immunology / Q000472:pathogenicity*; D013047:Specific Pathogen-Free Organisms; D013154:Spleen / Q000473:pathology / Q000821:virology; D013950:Thymus Gland / Q000473:pathology / Q000821:virology; D014774:Virulence
chemical_list: D000956:Antigens, Viral; D053148:Caspase 3