title: Detection of renal allograft dysfunction with characteristic protein fingerprint by serum proteomic analysis.
abstract: This study aimed to diagnose renal allograft dysfunction with specific biomarkers by serum proteomic analysis. Surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) and bioinformatics (support vector machine and leave-one cross validation) were used to analyze serum proteome. Enrolled patients included 38 biopsy-proved acute rejection (BPAR), 10 acute tubular necrosis (ATN), 24 subclinical rejection (SCR) and 29 stable control recipients verified by protocol biopsy. A characteristic protein profile can be detected in each renal allograft dysfunction group. BPAR patients were differentiated from stable patients with markers of 9710.1, 4971, 6675.5, 8563.8, 6709.2, 9319 and 4476.7 Da with high sensitivity and specificity. ATN can be clearly distinguished from BPAR and stable control. Subclinical rejection differentiated from stable control with markers of 9193.1, 2759.1, 8464.6 Da. The independent blind test yielded with high specificity and sensitivity for each group. Serum proteome analysis by SELDI-TOF MS combined with bioinformatics in renal allograft dysfunction is valuable and promising. Specific markers were detected in each group. Identification of these proteins may prove useful as diagnostic markers for allograft dysfunction and better to elucidate the mechanism of acute rejection.
authors.affiliation: The Kidney Disease Center, The First Affiliated Hospital of Medical College, Zhejiang University, 79 Qingchun Road, Hangzhou, China.
authors.forename: Minmin
authors.initials: M
authors.lastname: Wang
authors.forename: Qiu
authors.initials: Q
authors.lastname: Jin
authors.forename: Haiyan
authors.initials: H
authors.lastname: Tu
authors.forename: Youying
authors.initials: Y
authors.lastname: Mao
authors.forename: Jiekai
authors.initials: J
authors.lastname: Yu
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.forename: Zhangfei
authors.initials: Z
authors.lastname: Shou
authors.forename: Qiang
authors.initials: Q
authors.lastname: He
authors.forename: Jianyong
authors.initials: J
authors.lastname: Wu
authors.forename: Shu
authors.initials: S
authors.lastname: Zheng
authors.forename: Jianghua
authors.initials: J
authors.lastname: Chen
mesh_terms: D000328:Adult; D000704:Analysis of Variance; D058070:Asymptomatic Diseases; D015415:Biomarkers / Q000097:blood; D001706:Biopsy; D019295:Computational Biology; D005260:Female; D006084:Graft Rejection / Q000097:blood* / Q000175:diagnosis* / Q000473:pathology; D006801:Humans; D016030:Kidney Transplantation / Q000473:pathology* / Q000502:physiology; D007683:Kidney Tubular Necrosis, Acute / Q000097:blood / Q000175:diagnosis; D008297:Male; D008875:Middle Aged; D010449:Peptide Mapping; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D055815:Young Adult
chemical_list: D015415:Biomarkers