title: Gene expression differences in healthy brachial and femoral arteries of Rapacz familial hypercholesterolemic swine.
abstract: The mechanisms underlying the unequal distribution of atherosclerotic disease in the peripheral arteries are currently unclear. Gene expression differences in healthy arteries may influence the heterogeneous distribution of atherosclerosis. Therefore, this investigation compares gene expression in healthy atheroprotected brachial and atherosusceptible femoral arteries of young and disease free Rapacz familial hypercholesterolemic (FHC) swine. We hypothesized that transcripts related to atherosusceptibility would be differentially expressed between these arteries prior to the onset of disease. Femoral and brachial arteries were harvested from four 13-day-old Rapacz FHC swine. No atherosclerotic disease was detected using Sudan IV, Verhoeff-van Gieson, and hematoxylin-eosin staining. Gene expression was quantified using Affymetrix GeneChip Porcine Genome Arrays. An average of 15,552 probe sets had detectable transcripts, while 430 probe sets showed a significant differential expression between arteries (false discovery rate < 0.05). The human orthologs of 63 probe sets with differential expression and a 1.5-fold or greater transcript abundance between arteries are associated with Wnt/β-catenin, lysophospholipid, and Ca-signaling, as well as apoptosis. This is the first investigation reporting that differences in relative abundance of gene expression exist between brachial and femoral arteries in young Rapacz FHC swine prior to the development of atherosclerotic lesions.
authors.affiliation: Departments of Health and Kinesiology, Purdue University, West Lafayette, Indiana, USA.
authors.forename: Martin
authors.initials: M
authors.lastname: Bahls
authors.forename: Christopher A
authors.initials: CA
authors.lastname: Bidwell
authors.forename: Juan
authors.initials: J
authors.lastname: Hu
authors.forename: Christian G
authors.initials: CG
authors.lastname: Krueger
authors.forename: Jess D
authors.initials: JD
authors.lastname: Reed
authors.forename: Armando
authors.initials: A
authors.lastname: Tellez
authors.forename: Greg L
authors.initials: GL
authors.lastname: Kaluza
authors.forename: Juan F
authors.initials: JF
authors.lastname: Granada
authors.forename: William G
authors.initials: WG
authors.lastname: Van Alstine
authors.forename: Sean C
authors.initials: SC
authors.lastname: Newcomer
mesh_terms: D000818:Animals; D001916:Brachial Artery / Q000378:metabolism*; D020013:Calcium Signaling / Q000235:genetics / Q000502:physiology*; D017931:DNA Primers / Q000235:genetics; D005263:Femoral Artery / Q000378:metabolism*; D020869:Gene Expression Profiling / Q000662:veterinary; D005786:Gene Expression Regulation / Q000502:physiology*; D006652:Histological Techniques / Q000662:veterinary; D006938:Hyperlipoproteinemia Type II / Q000235:genetics / Q000378:metabolism / Q000662:veterinary*; D008246:Lysophospholipids / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis / Q000662:veterinary; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000235:genetics / Q000378:metabolism*; D051153:Wnt Proteins / Q000378:metabolism; D051176:beta Catenin / Q000378:metabolism
chemical_list: D017931:DNA Primers; D008246:Lysophospholipids; D051153:Wnt Proteins; D051176:beta Catenin