title: Nanoneedle transistor-based sensors for the selective detection of intracellular calcium ions.
abstract: We developed a nanoneedle transistor-based sensor (NTS) for the selective detection of calcium ions inside a living cell. In this work, a single-walled carbon nanotube-based field effect transistor (swCNT-FET) was first fabricated at the end of a glass nanopipette and functionalized with Fluo-4-AM probe dye. The selective binding of calcium ions onto the dye molecules altered the charge state of the dye molecules, resulting in the change of the source-drain current of the swCNT-FET as well as the fluorescence intensity from the dye. We demonstrated the electrical and fluorescence detection of the concentration change of intracellular calcium ions inside a HeLa cell using the NTS.
authors.affiliation: Interdisciplinary Program in Nano-Science and Technology, Seoul National University, Seoul 151-748, Korea.
authors.forename: Donghee
authors.initials: D
authors.lastname: Son
authors.forename: Sung Young
authors.initials: SY
authors.lastname: Park
authors.forename: Byeongju
authors.initials: B
authors.lastname: Kim
authors.forename: Jun Tae
authors.initials: JT
authors.lastname: Koh
authors.forename: Tae Hyun
authors.initials: TH
authors.lastname: Kim
authors.forename: Sangmin
authors.initials: S
authors.lastname: An
authors.forename: Doyoung
authors.initials: D
authors.lastname: Jang
authors.forename: Gyu Tae
authors.initials: GT
authors.lastname: Kim
authors.forename: Wonho
authors.initials: W
authors.lastname: Jhe
authors.forename: Seunghun
authors.initials: S
authors.lastname: Hong
mesh_terms: D000669:Amplifiers, Electronic; D015374:Biosensing Techniques / Q000295:instrumentation*; D002118:Calcium / Q000032:analysis*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006367:HeLa Cells; D006801:Humans; D007477:Ions; D036103:Nanotechnology / Q000295:instrumentation*; D009339:Needles; D014173:Transistors, Electronic
chemical_list: D007477:Ions; D002118:Calcium