title: Immunomodulating potency of lipopolysaccharides (LPS) derived from smooth type of bacterial pathogens in Indian major carp.
abstract: Lipopolysaccharides (LPS), the outer membrane of the Gram-negative bacteria, are reported to stimulate the immunity of different vertebrates including fish. However, their potency and spectrum of actions often differ among different bacteria. In this study, effect of crude LPS, derived from three species of smooth Gram-negative bacterial fish pathogens viz. Edwardsiella tarda, Escherichia coli, and Pseudomonas fluorescens, on certain innate immune parameters of Indian major carp, Labeo rohita was studied. L. rohita yearlings, when injected intraperitoneally with crude LPS extracted from these bacteria showed little variations in different innate immune parameters. Furthermore, LPS injected fish were protected against a virulent E. tarda challenge. Although, no significant difference (p>0.05) in most of the immune parameters were found with LPS of different bacteria, the E. coli LPS injected fish elucidated high resistivity during challenge study. Hence, there could be some variations in LPS with respect to the bacterial type which needs to be further explored.
authors.affiliation: Laboratory of Fish Pathology, Department of Veterinary Medicine, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-8510, Japan. sukantanayak@rediffmail.com
authors.forename: S K
authors.initials: SK
authors.lastname: Nayak
authors.forename: P
authors.initials: P
authors.lastname: Swain
authors.forename: P K
authors.initials: PK
authors.lastname: Nanda
authors.forename: D
authors.initials: D
authors.lastname: Mohapatra
authors.forename: T
authors.initials: T
authors.lastname: Behera
mesh_terms: D000818:Animals; D002347:Carps / Q000276:immunology*; D020609:Edwardsiella tarda / Q000276:immunology / Q000472:pathogenicity; D004926:Escherichia coli / Q000276:immunology; D007113:Immunity, Innate; D056747:Immunomodulation; D007274:Injections, Intraperitoneal; D008070:Lipopolysaccharides / Q000276:immunology*; D011551:Pseudomonas fluorescens / Q000276:immunology
chemical_list: D008070:Lipopolysaccharides