title: Exercise affects both ovarian follicular dynamics and hormone concentrations in mares.
abstract: The objectives were to evaluate the effects of exercise on ovarian folliculogenesis and related hormones in mares. Mares (n = 11) were randomly assigned into a control (non-exercised) or treatment (exercised) group. Treatment mares (n = 5) were moderately exercised for 30 min, 6 d/wk. All mares underwent daily transrectal ultrasonographic examinations and ovarian follicles > 6 mm were measured. Blood samples were collected during the first (Cycle 1) and last (Cycle 4) cycle, and serum concentrations of cortisol, LH, and FSH were determined. Mean cortisol concentrations were elevated (P < 0.05) in exercised mares, 6.29 ± 0.22 compared with 5.62 ± 0.16 ng/dL (mean ± SEM), 30 min post exercise. There were no significant differences between groups in mean FSH concentrations; however, exercised mares had lower (17.3 ± 6.4 vs 41.1 ± 5.5 ng/mL; P < 0.05) peak LH concentrations. Furthermore, exercised mares experienced a longer (24.7 ± 0.8 vs 22.2 ± 0.8 d; P < 0.05) mean interovulatory interval for all cycles combined, fewer (P < 0.05) follicles 6 to 20 mm in diameter, and an increased (P < 0.05) number of follicles >20 mm following deviation. The dominant and largest subordinate follicle in exercised mares had a greater (P < 0.05) mean diameter on the day of deviation, suggesting delayed deviation. Exercised mares also tended (P = 0.06) to have an increased number of cycles with at least two dominant follicles compared to control (62 vs 36%, respectively), indicating a decreased ability of the largest follicle to assert dominance. Under the conditions of this study, moderately exercising mares induced higher cortisol concentrations, lowered peak LH concentrations, and altered ovarian follicular dynamics.
authors.affiliation: Department of Animal Sciences, University of Florida, Florida, USA. dekelle@ufl.edu
authors.forename: D E
authors.initials: DE
authors.lastname: Kelley
authors.forename: J R
authors.initials: JR
authors.lastname: Gibbons
authors.forename: R
authors.initials: R
authors.lastname: Smith
authors.forename: K L
authors.initials: KL
authors.lastname: Vernon
authors.forename: S E
authors.initials: SE
authors.lastname: Pratt-Phillip
authors.forename: C J
authors.initials: CJ
authors.lastname: Mortensen
mesh_terms: D000818:Animals; D016009:Chi-Square Distribution; D030762:Estrous Cycle / Q000502:physiology; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood*; D006736:Horses / Q000097:blood / Q000502:physiology*; D006854:Hydrocortisone / Q000097:blood*; D007986:Luteinizing Hormone / Q000097:blood*; D006080:Ovarian Follicle / Q000000981:diagnostic imaging / Q000502:physiology*; D010060:Ovulation / Q000502:physiology; D010805:Physical Conditioning, Animal; D011897:Random Allocation; D014463:Ultrasonography
chemical_list: D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D006854:Hydrocortisone