title: Cytogenetic and molecular characterization of a hepatosplenic T-cell lymphoma: report of a novel chromosomal aberration.
abstract: Hepatosplenic T-cell lymphomas (HSTCL) are rare cancers and comprise 5% of peripheral T-cell lymphomas. These well-characterized extranodal lymphomas have a disguised onset, secondary to intrasinusoidal infiltration of the spleen, liver, and bone marrow, with a rapidly progressive course that is poorly responsive to chemotherapy and often ensues in the setting of immune system suppression. We describe the clinical, immunophenotypic, cytogenetic, fluorescence in situ hybridization, and molecular analyses for T cell receptor gene rearrangement in a 21-year-old man diagnosed with HSTCL. Immunophenotypic analysis revealed negativity for CD5 as well as double negativity for CD4/CD8 mature T-cell immunophenotype, which suggested the diagnosis of hepatosplenic T-cell lymphoma. Molecular analysis confirmed a TCR gene rearrangement, thereby verifying the common T-cell origin of the present HSTCL case. Furthermore, cytogenetic analysis revealed a novel chromosomal rearrangement, t(7;15)(p22;q21). Metaphase fluorescence in situ hybridization analysis confirmed the translocation of a chromosomal segment from 15q21 to 7p22.
authors.affiliation: Cytogenetics Division, Super Religare Laboratories, Mumbai, India. swarna@srl.in
authors.forename: Swarna
authors.initials: S
authors.lastname: Mandava
authors.forename: Reshma
authors.initials: R
authors.lastname: Sonar
authors.forename: Firoz
authors.initials: F
authors.lastname: Ahmad
authors.forename: Anil K
authors.initials: AK
authors.lastname: Yadav
authors.forename: Pratiksha
authors.initials: P
authors.lastname: Chheda
authors.forename: Manisha
authors.initials: M
authors.lastname: Ramani
authors.forename: Amar D
authors.initials: AD
authors.lastname: Gupta
authors.forename: Bibhu R
authors.initials: BR
authors.lastname: Das
mesh_terms: D002869:Chromosome Aberrations; D019674:Genes, T-Cell Receptor beta; D019675:Genes, T-Cell Receptor gamma; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology*; D016399:Lymphoma, T-Cell / Q000235:genetics* / Q000473:pathology*; D008297:Male; D013160:Splenic Neoplasms / Q000235:genetics* / Q000473:pathology*; D055815:Young Adult