title: Distinct MHC class I-dependent NK cell-activating receptors control cytomegalovirus infection in different mouse strains.
abstract: Recognition of mouse cytomegalovirus (MCMV)-infected cells by activating NK cell receptors was first described in the context of Ly49H, which confers resistance to C57BL/6 mice. We investigated the ability of other activating Ly49 receptors to recognize MCMV-infected cells in mice from various H-2 backgrounds. We observed that Ly49P1 from NOD/Ltj mice, Ly49L from BALB mice, and Ly49D2 from PWK/Pas mice respond to MCMV-infected cells in the context of H-2D(k) and the viral protein m04/gp34. Recognition was also seen in the H-2(d) and/or H-2(f) contexts, depending on the Ly49 receptor examined, but never in H-2(b). Furthermore, BALB.K (H-2(k)) mice showed reduced viral loads compared with their H-2(d) or H-2(b) congenic partners, a reduction which was dependent on interferon γ secretion by Ly49L(+) NK cells early after infection. Adoptive transfer of Ly49L(+), but not Ly49L(-), NK cells significantly increased resistance against MCMV infection in neonate BALB.K mice. These results suggest that multiple activating Ly49 receptors participate in H-2-dependent recognition of MCMV infection, providing a common mechanism of NK cell-mediated resistance against viral infection.
authors.affiliation: Department of Human Genetics, McGill University, Montreal, Quebec H3A 2T5, Canada.
authors.forename: Michał
authors.initials: M
authors.lastname: Pyzik
authors.forename: Benoit
authors.initials: B
authors.lastname: Charbonneau
authors.forename: Eve-Marie
authors.initials: EM
authors.lastname: Gendron-Pontbriand
authors.forename: Marina
authors.initials: M
authors.lastname: Babić
authors.forename: Astrid
authors.initials: A
authors.lastname: Krmpotić
authors.forename: Stipan
authors.initials: S
authors.lastname: Jonjić
authors.forename: Silvia M
authors.initials: SM
authors.lastname: Vidal
mesh_terms: D000818:Animals; D006566:Herpesviridae Infections / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D015395:Histocompatibility Antigens Class I / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D007113:Immunity, Innate; D007694:Killer Cells, Natural / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D016688:Mice, Inbred NOD; D018146:Muromegalovirus / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D055650:NK Cell Lectin-Like Receptor Subfamily A / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D013045:Species Specificity; D019562:Viral Load / Q000276:immunology
chemical_list: D015395:Histocompatibility Antigens Class I; C527096:Klra8 protein, mouse; D055650:NK Cell Lectin-Like Receptor Subfamily A