title: [Structure determination of five trace chemical constituents from roots of Linum usitatissimum].
abstract: OBJECTIVE
  To investigate the trace chemical constituents from the roots of Linum usitatissimum.
  
  
  METHODS
  Isolation and purification of the trace constituents were carried out mainly by solvents extraction and macroporous adsorbing resin and silica gel column chromatography. The structures of the isolates were elucidated by extensive spectroscopic analysis,including 1D and 2D NMR, MS, and HRMS.
  
  
  RESULTS
  Five trace compounds were isolated and identified as 3-methyl-2, 5-pyrrolidinedione-5-oxime (1), hypoxanthine (2), nonanedioic acid-1,9 (3), and the mixture of isoleucine and leucine(4 and 5).
  
  
  CONCLUSIONS
  Compound 1 is a new compound, and compounds 2-5 are isolated from L. usitatissimum for the first time.
authors.affiliation: Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
authors.forename: Jianlong
authors.initials: J
authors.lastname: Sun
authors.forename: Anjun
authors.initials: A
authors.lastname: Deng
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Li
authors.forename: Hailin
authors.initials: H
authors.lastname: Qin
mesh_terms: D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification; D019597:Flax / Q000737:chemistry*; D015394:Molecular Structure; D018517:Plant Roots / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal