title: The phosphate transporter gene OsPht1;8 is involved in phosphate homeostasis in rice.
abstract: Plant phosphate transporters (PTs) are active in the uptake of inorganic phosphate (Pi) from the soil and its translocation within the plant. Here, we report on the biological properties and physiological roles of OsPht1;8 (OsPT8), one of the PTs belonging to the Pht1 family in rice (Oryza sativa). Expression of a β-glucuronidase and green fluorescent protein reporter gene driven by the OsPT8 promoter showed that OsPT8 is expressed in various tissue organs from roots to seeds independent of Pi supply. OsPT8 was able to complement a yeast Pi-uptake mutant and increase Pi accumulation of Xenopus laevis oocytes when supplied with micromolar (33)Pi concentrations at their external solution, indicating that it has a high affinity for Pi transport. Overexpression of OsPT8 resulted in excessive Pi in both roots and shoots and Pi toxic symptoms under the high-Pi supply condition. In contrast, knockdown of OsPT8 by RNA interference decreased Pi uptake and plant growth under both high- and low-Pi conditions. Moreover, OsPT8 suppression resulted in an increase of phosphorus content in the panicle axis and in a decrease of phosphorus content in unfilled grain hulls, accompanied by lower seed-setting rate. Altogether, our data suggest that OsPT8 is involved in Pi homeostasis in rice and is critical for plant growth and development.
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Hongfang
authors.initials: H
authors.lastname: Jia
authors.forename: Hongyan
authors.initials: H
authors.lastname: Ren
authors.forename: Mian
authors.initials: M
authors.lastname: Gu
authors.forename: Jianning
authors.initials: J
authors.lastname: Zhao
authors.forename: Shubin
authors.initials: S
authors.lastname: Sun
authors.forename: Xiao
authors.initials: X
authors.lastname: Zhang
authors.forename: Jieyu
authors.initials: J
authors.lastname: Chen
authors.forename: Ping
authors.initials: P
authors.lastname: Wu
authors.forename: Guohua
authors.initials: G
authors.lastname: Xu
mesh_terms: D000818:Animals; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics*; D006706:Homeostasis / Q000187:drug effects / Q000235:genetics*; D008969:Molecular Sequence Data; D009865:Oocytes / Q000187:drug effects / Q000378:metabolism; D009928:Organ Specificity / Q000187:drug effects; D012275:Oryza / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D028061:Phosphate Transport Proteins / Q000235:genetics* / Q000378:metabolism; D010710:Phosphates / Q000172:deficiency / Q000378:metabolism* / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D021381:Protein Transport / Q000187:drug effects; D012441:Saccharomyces cerevisiae; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism; D013489:Suppression, Genetic / Q000187:drug effects; D014981:Xenopus
chemical_list: D028061:Phosphate Transport Proteins; D010710:Phosphates; D010940:Plant Proteins