title: Detection of trace proteins in multicomponent mixtures using displacement chromatography.
abstract: Model protein feed mixtures containing three abundant and seven trace proteins at various concentrations were identified and employed in a series of displacement experiments. Reversed-phase liquid chromatography (RPLC) and matrix assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry were used to evaluate the compositions of both the feed mixtures and effluent fractions from the displacement experiments. The results demonstrated that trace proteins were focused at the boundaries between the abundant solutes where they were enriched and concentrated. For many of the multicomponent feed mixtures, mass spectrometry analyses of the displacement column effluent fractions resulted in the identification of trace proteins that were not detectable in the feed. In addition, the use of minimal or no salt in the carrier solutions enabled the analysis of displacement fractions by direct infusion mass spectrometry. These results are significant in that they indicate that while the presence of abundant proteins can often be problematic for the detection of trace components, displacement chromatography may be able to employ these abundant proteins to focus trace proteins in the displacement train, thus facilitating detection.
authors.affiliation: Department of Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, New York 12180, USA.
authors.forename: Steven T
authors.initials: ST
authors.lastname: Evans
authors.forename: Melissa
authors.initials: M
authors.lastname: Holstein
authors.forename: Steven M
authors.initials: SM
authors.lastname: Cramer
mesh_terms: D000818:Animals; D007611:Aprotinin / Q000032:analysis; D002256:Carbonic Anhydrases / Q000032:analysis; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D005969:Glutamate Dehydrogenase / Q000032:analysis; D007328:Insulin / Q000032:analysis; D009113:Muramidase / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D012710:Serum Albumin, Bovine / Q000032:analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D014361:Trypsin Inhibitors / Q000032:analysis
chemical_list: D007328:Insulin; D011506:Proteins; D014361:Trypsin Inhibitors; D012710:Serum Albumin, Bovine; D007611:Aprotinin; D005969:Glutamate Dehydrogenase; D009113:Muramidase; D002256:Carbonic Anhydrases