title: Artificial-epitope mapping for CK-MB assay.
abstract: A quantitative method using artificial antibody to detect creatine kinases was developed. Linear epitope sequences were selected based on an artificial-epitope mapping strategy. Nine different MIPs corresponding to the selected peptides were then fabricated on QCM chips. The subtle conformational changes were also recognized by these chips.
authors.affiliation: Department of Chemistry, National Dong-Hwa University, Hualien, Taiwan. dftai@mail.ndhu.edu.tw
authors.forename: Dar-Fu
authors.initials: DF
authors.lastname: Tai
authors.forename: Yi-Fang
authors.initials: YF
authors.lastname: Ho
authors.forename: Cheng-Hsin
authors.initials: CH
authors.lastname: Wu
authors.forename: Tzu-Chieh
authors.initials: TC
authors.lastname: Lin
authors.forename: Kuo-Hao
authors.initials: KH
authors.lastname: Lu
authors.forename: Kun-Shian
authors.initials: KS
authors.lastname: Lin
mesh_terms: D000595:Amino Acid Sequence; D000906:Antibodies / Q000276:immunology; D052279:Creatine Kinase, MB Form / Q000737:chemistry*; D018604:Epitope Mapping / Q000379:methods; D054802:Molecular Imprinting / Q000379:methods*; D010455:Peptides / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D020033:Protein Isoforms / Q000737:chemistry; D017434:Protein Structure, Tertiary; D058525:Quartz Crystal Microbalance Techniques
chemical_list: D000906:Antibodies; D010455:Peptides; D011108:Polymers; D020033:Protein Isoforms; D052279:Creatine Kinase, MB Form