title: Mass spectrometry detection as an innovative and advantageous tool in ligand exchange capillary electrophoresis.
abstract: The copper(II) complex of a modified cyclodextrin, namely 6-mono-deoxy-6-[4-(2-aminoethyl)imidazolyl]-β-CD (CDmh), previously synthesized and characterized in our laboratory and already used as chiral selector for ligand exchange capillary electrophoresis (LECE) with optical detection, is investigated here in LECE using electrospray-mass spectrometry (ESI-MS) as the detection device. The potential of this hyphenated method is compared with the results previously obtained with optical detection by studying the chiral resolution of tryptophan racemate. Chiral separation conditions compatible with LECE-ESI-MS could be achieved based on the figures of merit obtained by LECE-UV. Interestingly, the values of LOD obtained by LECE-ESI-MS were significantly better than those obtained by LECE-UV and thus, ESI-MS detection seems to open new perspectives in chiral separations by LECE.
authors.affiliation: Department of Chemical Sciences, University of Catania, viale Andrea Doria, Catania, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Giuffrida
authors.forename: Annalinda
authors.initials: A
authors.lastname: Contino
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Maccarrone
authors.forename: Marianna
authors.initials: M
authors.lastname: Messina
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Cucinotta
mesh_terms: D056831:Coordination Complexes / Q000737:chemistry; D003300:Copper / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D007093:Imidazoles / Q000737:chemistry; D008024:Ligands; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013237:Stereoisomerism; D014364:Tryptophan / Q000737:chemistry; D047392:beta-Cyclodextrins / Q000737:chemistry
chemical_list: D056831:Coordination Complexes; D007093:Imidazoles; D008024:Ligands; D047392:beta-Cyclodextrins; D003300:Copper; D014364:Tryptophan