title: Retro-orbital injections in mice.
abstract: Intravenous vascular access is technically challenging in the adult mouse and even more challenging in neonatal mice. The authors describe the technique of retro-orbital injection of the venous sinus in the adult and neonatal mouse. This technique is a useful alternative to tail vein injection for the administration of non-tumorigenic compounds. The authors report that they have routinely used this technique in the adult mouse to administer volumes up to 150 μl without incident. Administration of retro-orbital injections is more challenging in neonatal mice but can reliably deliver volumes up to 10 μl.
authors.affiliation: Medical Genetics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, USA.
authors.forename: Tal
authors.initials: T
authors.lastname: Yardeni
authors.forename: Michael
authors.initials: M
authors.lastname: Eckhaus
authors.forename: H Douglas
authors.initials: HD
authors.lastname: Morris
authors.forename: Marjan
authors.initials: M
authors.lastname: Huizing
authors.forename: Shelley
authors.initials: S
authors.lastname: Hoogstraten-Miller
mesh_terms: D000758:Anesthesia; D000818:Animals; D000831:Animals, Newborn; D007275:Injections, Intravenous / Q000295:instrumentation / Q000379:methods*; D007756:Laboratory Animal Science / Q000379:methods*; D051379:Mice; D009915:Orbit / Q000098:blood supply* / Q000473:pathology