title: Hydroxycinnamic acid amide derivatives, phenolic compounds and antioxidant activities of extracts of pollen samples from Southeast Brazil.
abstract: Seven bee pollen samples (C1-C7) with different palynological sources were harvested from Pindamonhangaba municipality (Southeast Brazil). Methanol extracts of untreated samples (control), samples frozen at -18 °C and samples frozen and then dried were analyzed by HPLC/PAD/ESI/MS/MS. Flavonoid diglycosides of quercetin, kaempferol, isorhamnetin and patuletin were detected, together with hydroxycinnamic acid amide derivatives, such as N',N'',N'''-tris-p-feruloylspermidine and N',N'',N'''-tris-p-coumaroylspermidine. Distinct phenolic profiles characterized the analyzed samples, but no differences were noted as resulting from different treatments. Total phenolic contents determined with the Folin-Ciocalteau reagent ranged from 1.7 to 2.2%. Antioxidant activities above 75%, based on the DPPH method, were observed for all extracts, not correlated with total phenolic contents. Among samples from the same origin, those frozen were more active than samples untreated (control), and the samples frozen and then dried were the most active.
authors.affiliation: Department of Psychobiology, Federal University of São Paulo-CEBRID-UNIFESP, São Paulo, Brazil. gnegri@terra.com.br
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Negri
authors.forename: Erica Weinstein
authors.initials: EW
authors.lastname: Teixeira
authors.forename: Maria Luisa Teles Marques Florêncio
authors.initials: ML
authors.lastname: Alves
authors.forename: Augusta Carolina de Camargo Carmello
authors.initials: AC
authors.lastname: Moreti
authors.forename: Ivani Pozar
authors.initials: IP
authors.lastname: Otsuk
authors.forename: Renata Galhardo
authors.initials: RG
authors.lastname: Borguini
authors.forename: Antonio
authors.initials: A
authors.lastname: Salatino
mesh_terms: D000975:Antioxidants / Q000032:analysis*; D001938:Brazil; D003373:Coumaric Acids / Q000032:analysis*; D005419:Flavonoids / Q000032:analysis; D010636:Phenols / Q000032:analysis*; D010936:Plant Extracts / Q000737:chemistry*; D011058:Pollen / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D003373:Coumaric Acids; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts