title: Investigating Nitrosomonas europaea stress biomarkers in batch, continuous culture, and biofilm reactors.
abstract: The understanding of nitrification inhibition in ammonia oxidizing bacteria (AOB) by priority pollutants and emerging contaminants is critical in managing the nitrogen cycle to preserve current water supplies, one of the National Academy of Engineers Grand Challenges in Engineering for the twenty-first century. Nitrosomonas europaea is an excellent model AOB for nitrification inhibition experimentation due to its well-defined NH(3) metabolism and the availability of a wide range of physiological and transcriptional tools that can characterize the mechanism of nitrification inhibition and probe N. europaea's response to the inhibitor. This chapter is a compilation of the physiological and transcriptional methods that have been used to characterize nitrification inhibition of N. europaea under a wide variety of growth conditions including batch, continuously cultured, and in biofilms. The protocols presented here can be applied to other AOB, and may be readily adapted for other autotrophic bacteria (e.g., nitrite oxidizing bacteria).
authors.affiliation: School of Chemical, Biological and Environmental Engineering, Oregon State University, Corvallis, Oregon, USA.
authors.forename: Tyler S
authors.initials: TS
authors.lastname: Radniecki
authors.forename: Ellen G
authors.initials: EG
authors.lastname: Lauchnor
mesh_terms: D000641:Ammonia / Q000378:metabolism; D052818:Autotrophic Processes; D018441:Biofilms / Q000254:growth & development*; D015415:Biomarkers / Q000032:analysis; D019149:Bioreactors; D018929:Cell Culture Techniques; D009584:Nitrogen / Q000378:metabolism; D058458:Nitrogen Cycle; D009586:Nitrogen Fixation / Q000235:genetics / Q000502:physiology; D043370:Nitrosomonas europaea / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013312:Stress, Physiological
chemical_list: D015415:Biomarkers; D012336:RNA, Ribosomal, 16S; D000641:Ammonia; D009584:Nitrogen