title: [Characteristics of Mycobacterium tuberculosis genotype and the relationship between Beijing genotype and drug-resistant phenotypes in Tianjin].
abstract: OBJECTIVE
  To explore the distribution and characteristics on genotype of Mycobacterium tuberculosis and the relationship between Beijing genotype and drug-resistant phenotypes in Tianjin city.
  
  
  METHODS
  656 clinical strains were collected from Tianjin Center for Tuberculosis Control and ten other Tuberculosis Institute in Tianjin from January 2008 to June 2009. Information regarding administration, clinical as well as laboratory findings of patients were collected. Proportion method was adopted to detect the susceptibility on four anti-tuberculosis drugs, namely streptomycin (SM), isoniazid (INH), rifampicin (RFP) and ethambutol (EMB). Both Beijing and non-Beijing genotypes were differentiated by multiplex PCR. The relationship between Beijing genotype and drug-resistant phenotypes was analyzed.
  
  
  RESULTS
  In this study, the overall resistance rate of MTB was 26.98%, with multidrug-resistant rate was 6.25%. Among 656 MTB strains, 600 isolates (91.46%) belonged to Beijing genotype. There was significant difference between Beijing and non-Beijing genotype (χ(2) = 4.26, P = 0.039) among the Tianjin household registered population. Concerning the drug resistance, there was no significant difference between the two groups.
  
  
  CONCLUSIONS
  Beijing genotype strains were the predominant one in Tianjin. The proportion of people infected with the Beijing genotype strains in Tianjin household registration of patients was significantly higher than the proportion of patients in the floating population in the same region. Results from the statistical analysis did not reveal any statistically significant association between Beijing genotype and drug resistance.
authors.affiliation: Tianjin Center for Tuberculosis Control, China.
authors.forename: Han-fang
authors.initials: HF
authors.lastname: Ju
authors.forename: Xie-xiu
authors.initials: XX
authors.lastname: Wang
authors.forename: Gui-lian
authors.initials: GL
authors.lastname: Li
authors.forename: Tong
authors.initials: T
authors.lastname: Xie
authors.forename: De-fu
authors.initials: DF
authors.lastname: Zhao
authors.forename: Shang-lun
authors.initials: SL
authors.lastname: Li
authors.forename: Jing-xin
authors.initials: JX
authors.lastname: Li
authors.forename: Hui
authors.initials: H
authors.lastname: Zhao
authors.forename: Cheng
authors.initials: C
authors.lastname: Mu
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002681:China / Q000453:epidemiology; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D010641:Phenotype; D018088:Tuberculosis, Multidrug-Resistant / Q000453:epidemiology* / Q000382:microbiology*; D055815:Young Adult