title: [Comparative evaluation of five test kits for antibody detection in Schistosoma japonicum endemic areas of Poyang Lake region].
abstract: OBJECTIVE
  To evaluate the efficacy of five diagnostic test kits for the detection of antibodies to Schistosoma japonicum in endemic areas of Poyang Lake.
  
  
  METHODS
  Residents over 5 years old in three schistosomiasis-endemic administrative villages in the lake region were simultaneously examined by modified Kato-Katz thick smears and nylon bag sedimentation/hatching method. At the same time, five sero-diagnostic test kits for detection of IgG antibody against soluble egg antigen of S. japonicum were carried out.
  
  
  RESULTS
  The distribution of seropositive rates of S. japonicum IgG antibodies determined by sero-diagnostic tests showed similar trends with that of egg positive rates in different age groups, but the seropositive rate (33.1%-57.1%) was significantly higher than that of egg positive rate (5.0%-8.2%), both reached a peak in 40-49-year-old group. The antibody positive rates determined by each test increased with the infection intensity. The sensitivity of the evaluated test kits ranged from 81.2%-94.5%. 26.9% to 46.1% among those egg negatives were antibody positive by the kits. Furthermore, 5.5%-18.8% of the egg positives were antibody negative by the kits.
  
  
  CONCLUSIONS
  The five sero-diagnostic kits can be used for epidemiological survey in schistosomiasis endemic areas at community level. Among them, enzyme-linked immunosorbent assay shows higher sensitivity.
authors.affiliation: Jiangxi Institute of Parasitic Diseases, Nanchang 330046, China.
authors.forename: Dan-Dan
authors.initials: DD
authors.lastname: Lin
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
authors.forename: Hong-Yun
authors.initials: HY
authors.lastname: Liu
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Zeng
authors.forename: Yue-Min
authors.initials: YM
authors.lastname: Liu
authors.forename: Shu-Ying
authors.initials: SY
authors.lastname: Xie
authors.forename: Fei
authors.initials: F
authors.lastname: Hu
authors.forename: Jian-Ying
authors.initials: JY
authors.lastname: Li
authors.forename: Mei-Jiao
authors.initials: MJ
authors.lastname: Huang
authors.forename: Hong-Gen
authors.initials: HG
authors.lastname: Chen
authors.forename: Xiao-Nong
authors.initials: XN
authors.lastname: Zhou
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000909:Antibodies, Helminth / Q000097:blood / Q000302:isolation & purification*; D002648:Child; D002675:Child, Preschool; D004784:Environmental Monitoring / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011933:Reagent Kits, Diagnostic; D012554:Schistosomiasis japonica / Q000276:immunology*; D055815:Young Adult
chemical_list: D000909:Antibodies, Helminth; D011933:Reagent Kits, Diagnostic