title: STED-SPIM: Stimulated emission depletion improves sheet illumination microscopy resolution.
abstract: We demonstrate the first, to our knowledge, integration of stimulated emission depletion (STED) with selective plane illumination microscopy (SPIM). Using this method, we were able to obtain up to 60% improvements in axial resolution with lateral resolution enhancements in control samples and zebrafish embryos. The integrated STED-SPIM method combines the advantages of SPIM with the resolution enhancement of STED, and thus provides a method for fast, high-resolution imaging with >100 μm deep penetration into biological tissue.
authors.affiliation: Molecular Microscopy Group, Bio-Imaging Center, Rudolf Virchow Center, University of Würzburg, Würzburg, Germany.
authors.forename: Mike
authors.initials: M
authors.lastname: Friedrich
authors.forename: Qiang
authors.initials: Q
authors.lastname: Gan
authors.forename: Vladimir
authors.initials: V
authors.lastname: Ermolayev
authors.forename: Gregory S
authors.initials: GS
authors.lastname: Harms
mesh_terms: D000818:Animals; D004625:Embryo, Nonmammalian; D008029:Lighting / Q000379:methods*; D008853:Microscopy / Q000379:methods*; D055620:Optical Phenomena; D015027:Zebrafish / Q000196:embryology