title: Microarray analysis uncovers a role for Tip60 in nervous system function and general metabolism.
abstract: BACKGROUND
  Tip60 is a key histone acetyltransferase (HAT) enzyme that plays a central role in diverse biological processes critical for general cell function; however, the chromatin-mediated cell-type specific developmental pathways that are dependent exclusively upon the HAT activity of Tip60 remain to be explored.
  
  
  RESULTS
  Here, we investigate the role of Tip60 HAT activity in transcriptional control during multicellular development in vivo by examining genome-wide changes in gene expression in a Drosophila model system specifically depleted for endogenous dTip60 HAT function.
  
  
  CONCLUSIONS
  We show that amino acid residue E431 in the catalytic HAT domain of dTip60 is critical for the acetylation of endogenous histone H4 in our fly model in vivo, and demonstrate that dTip60 HAT activity is essential for multicellular development. Moreover, our results uncover a novel role for Tip60 HAT activity in controlling neuronal specific gene expression profiles essential for nervous system function as well as a central regulatory role for Tip60 HAT function in general metabolism.
authors.affiliation: Department of Biology, Drexel University, Philadelphia, Pennsylvania, United States of America.
authors.forename: Meridith
authors.initials: M
authors.lastname: Lorbeck
authors.forename: Keerthy
authors.initials: K
authors.lastname: Pirooznia
authors.forename: Jessica
authors.initials: J
authors.lastname: Sarthi
authors.forename: Xianmin
authors.initials: X
authors.lastname: Zhu
authors.forename: Felice
authors.initials: F
authors.lastname: Elefant
mesh_terms: D000107:Acetylation; D000818:Animals; D030801:Animals, Genetically Modified; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D005799:Genes, Dominant / Q000235:genetics; D017344:Genes, Insect / Q000235:genetics; D051548:Histone Acetyltransferases / Q000235:genetics / Q000378:metabolism*; D006657:Histones / Q000378:metabolism; D006801:Humans; D005810:Multigene Family / Q000235:genetics; D050505:Mutant Proteins; D009420:Nervous System / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D021381:Protein Transport; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic
chemical_list: D029721:Drosophila Proteins; D006657:Histones; D050505:Mutant Proteins; D051548:Histone Acetyltransferases; C511056:Tip60 protein, Drosophila