title: Thymus and activation-regulated chemokine (TARC)/CC chemokine ligand (CCL) 17 accelerates wound healing by enhancing fibroblast migration.
abstract: BACKGROUND
  Some chemokines are known to accelerate wound healing. However, there has been no report on the relationship between Thymus and activation-regulated chemokine (TARC)/CC chemokine ligand (CCL) 17 and wound healing. The purpose of this study was to determine whether CCL17 enhances response to cutaneous injury.
  
  
  METHODS
  We made a full-thickness dorsal wound in transgenic (Tg) mice, in which CCL17 was overexpressed and in control mice. Wound size was compared over the course of time. We evaluated the effect of CCL17 on fibroblast migration by a Boyden chamber assay and a scratch wound assay.
  
  
  RESULTS
  Wound closure in Tg mice was more accelerated than in control mice. CCL17 enhanced nerve growth factor (NGF) production by 2B4, which is mouse T cell hybridoma. Further, in the wound area of Tg mice, the number of CCR4(+) fibroblasts, CCR4(+) lymphocytes and mast cells was increased compared to control mice, as was the number of NGF(+) lymphocytes around the wound area. In vitro assay, CCL17 was shown to enhance the migration of fibroblasts.
  
  
  CONCLUSIONS
  These results suggest that CCL17 accelerates wound healing, mainly by enhancing fibroblast migration, and possibly by increasing NGF(+) lymphocytes and mast cells, which have independently been reported to enhance wound healing.
authors.affiliation: Department of Dermatology, Faculty of Medicine, University of Tokyo, Tokyo, Japan. tkatou-tky@umin.net
authors.forename: Toyoaki
authors.initials: T
authors.lastname: Kato
authors.forename: Hidehisa
authors.initials: H
authors.lastname: Saeki
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Tsunemi
authors.forename: Sayaka
authors.initials: S
authors.lastname: Shibata
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Tamaki
authors.forename: Shinichi
authors.initials: S
authors.lastname: Sato
mesh_terms: D000818:Animals; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology*; D049109:Cell Proliferation; D054414:Chemokine CCL17 / Q000235:genetics / Q000502:physiology*; D005347:Fibroblasts / Q000166:cytology* / Q000502:physiology; D008214:Lymphocytes / Q000166:cytology / Q000502:physiology; D008407:Mast Cells / Q000166:cytology / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D023421:Models, Animal; D020932:Nerve Growth Factor / Q000097:blood; D012867:Skin / Q000166:cytology / Q000293:injuries*; D013997:Time Factors; D014945:Wound Healing / Q000502:physiology*
chemical_list: C499538:Ccl17 protein, mouse; D054414:Chemokine CCL17; D020932:Nerve Growth Factor