title: Effects of ascorbate fatty ester derivatives on erythrocyte membrane lipoperoxidation.
abstract: 6-O-alkyl ascorbic acid esters (ASC(n)) are amphiphilic molecules that behave as surfactants in aqueous solution. ASC(n) have shown some physical and rheological properties that suggest a potential utility as drug carriers. The present paper aims to evaluate the effect of ASC(n) on erythrocyte properties in order to get information regarding the relationship between osmotic fragility, erythrocyte deformability and membrane lipoperoxidation process. The assays were performed at the following concentrations: the critical micelar concentration (CMC), producing 10% hemolysis (CH(10)) and producing 50% hemolysis (CH(50)). We observed that ASC(n) (ASC(8), ASC(10) and ASC(12)), at concentration nearby CMC, neither affected cell deformability nor produced lipoperoxidation. Nevertheless, at higher concentrations (CH(10) and CH(50)), the RBCs incubated with ASC(n) were affected by a significant and progressive loss of deformability, simultaneously with an increase of osmotic fragility and membrane lipoperoxidation.
authors.affiliation: Cátedra de Biofísica, Facultad de Ciencias Médicas, Universidad Nacional de Rosario, Argentina. isabelspengler@hotmail.com
authors.forename: M I
authors.initials: MI
authors.lastname: Spengler
authors.forename: M
authors.initials: M
authors.lastname: Rasia
authors.forename: S
authors.initials: S
authors.lastname: Palma
authors.forename: D
authors.initials: D
authors.lastname: Allemandi
mesh_terms: D000328:Adult; D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004907:Erythrocyte Deformability / Q000187:drug effects; D004910:Erythrocyte Membrane / Q000187:drug effects* / Q000378:metabolism; D004952:Esters / Q000737:chemistry / Q000494:pharmacology; D005227:Fatty Acids / Q000097:blood*; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects*; D009996:Osmotic Fragility / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects
chemical_list: D004952:Esters; D005227:Fatty Acids; D001205:Ascorbic Acid