title: [Expression of the MyoD and m-cadherin genes in the myogenic precursor cell culture isolated from muscles of rats at different stages of ontogenesis].
abstract: Comparative analysis of expression of the MyoD gene and m-cadherin in the myogenic precursor cells isolated from rats' muscular tissue on the 20th-21st day of embryogenesis, on the third to fifth day of postnatal development, and from adult animals was carried out. No significant differences in expression of the @MyoD@[ital] gene were observed. Nevertheless, we found that in contrast to myogenic cells isolated from postnatal muscular tissues, in the subpopulation of myoblasts isolated from embryonic tissues on the 20th-21st day of embryogenesis cells that were more progressive with respect to differentiation prevailed. These cells demonstrate little proliferative activity alongside with expression of m-cadherin, the protein responsible for cytoadherence, and, as a consequence, a higher rate of differentiation.
authors.forename: O V
authors.initials: OV
authors.lastname: Balan
authors.forename: N D
authors.initials: ND
authors.lastname: Ozerniuk
mesh_terms: D000818:Animals; D015820:Cadherins / Q000096:biosynthesis / Q000235:genetics*; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000235:genetics; D049109:Cell Proliferation; D002478:Cells, Cultured; D018507:Gene Expression Regulation, Developmental; D007150:Immunohistochemistry; D024510:Muscle Development / Q000235:genetics*; D018485:Muscle Fibers, Skeletal / Q000378:metabolism; D018482:Muscle, Skeletal / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D017570:MyoD Protein / Q000096:biosynthesis / Q000235:genetics*; D032446:Myoblasts / Q000378:metabolism; D016133:Polymerase Chain Reaction; D051381:Rats
chemical_list: D015820:Cadherins; D017570:MyoD Protein; C070896:M-cadherin