title: Anticancer effects of novel photodynamic therapy with glycoconjugated chlorin for gastric and colon cancer.
abstract: OBJECTIVE
  Photodynamic therapy (PDT) is an attractive, minimally invasive modality for cancer therapy that utilizes the interaction of light and photosensitizer. To improve the efficacy of PDT, development of cancer specificity of the photosensitizer is needed. Cancer cells consume more glucose than normal cells. In this study, the efficacy of PDT using a newly developed photosensitizer, glycoconjugated chlorin (H2TFPC-SGlc), was compared with Talaporfin, which is clinically used in Japan.
  
  
  METHODS
  Photosensitizers were administered to gastric and colon cancer cell lines, followed by irradiation of light, and the cell death-inducing effects were compared. Xenograft tumor mouse models were established and photosensitizer accumulation was assessed and antitumor effects analyzed.
  
  
  RESULTS
  In vitro, H(2)TFPC-SGlc was 30 times more cytotoxic to cancer cells than was Talaporfin. In vivo, H(2)TFPC-SGlc accumulation was higher in xenograft tumors and significantly suppressed tumor growth when compared with Talaporfin.
  
  
  CONCLUSIONS
  This novel glycoconjugated chlorin is potentially useful in PDT.
authors.affiliation: Department of Gastroenterology and Metabolism, Nagoya City University Graduate School of Medical Sciences, 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 467-8601, Japan.
authors.forename: Mamoru
authors.initials: M
authors.lastname: Tanaka
authors.forename: Hiromi
authors.initials: H
authors.lastname: Kataoka
authors.forename: Motoshi
authors.initials: M
authors.lastname: Mabuchi
authors.forename: Shiho
authors.initials: S
authors.lastname: Sakuma
authors.forename: Satoru
authors.initials: S
authors.lastname: Takahashi
authors.forename: Ryoto
authors.initials: R
authors.lastname: Tujii
authors.forename: Haruo
authors.initials: H
authors.lastname: Akashi
authors.forename: Hiromi
authors.initials: H
authors.lastname: Ohi
authors.forename: Shigenobu
authors.initials: S
authors.lastname: Yano
authors.forename: Akimichi
authors.initials: A
authors.lastname: Morita
authors.forename: Takashi
authors.initials: T
authors.lastname: Joh
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003110:Colonic Neoplasms / Q000188:drug therapy* / Q000473:pathology; D006001:Glycoconjugates / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D006801:Humans; D020128:Inhibitory Concentration 50; D051379:Mice; D008819:Mice, Nude; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D011166:Porphyrins / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000473:pathology; D023041:Xenograft Model Antitumor Assays
chemical_list: D000970:Antineoplastic Agents; D006001:Glycoconjugates; D017319:Photosensitizing Agents; D011166:Porphyrins; D017382:Reactive Oxygen Species; C006969:chlorin