title: Bioactive components from the tea polyphenols influence on endogenous antioxidant defense system and modulate inflammatory cytokines after total-body irradiation in mice.
abstract: The present study aimed to evaluate the radioprotective efficacy of green tea polyphenols and the component ingredients against irradiated-induced damage in mice and elucidate the underlying mechanisms. Green tea polyphenols (GTP 50, 50 and 100 mg/kg, p.o. daily) and its four individual components (25 and 50 mg/kg, p.o. daily) were administrated to the irradiated-injured mice for 21 days. The radioprotective effect on the hematopoietic system, serum cytokines, and endogenous antioxidant enzymes was studied. GTP 50 significant revert the irradiated-induced decline in hematological parameters (RBCs, WBCs, Hb), meanwhile, protected antioxidant defense system, as evidenced by decreased of serum lipid peroxidation (malonyldialdehyde) and elevation the antioxidant enzyme superoxide dismutase (SOD). Among the GTP components, catechin showed the best effect on elevation of hematological parameters, and epigallocatechin gallate showed the best antioxidant activity. Moreover GTP and its bioactive components (catechin, epigallocatechin and epigallocatechin-3-gallate) assisted in decreasing the leukocytopenia seen after whole mice irradiation and significantly reduced the elevated serum inflammatory cytokines (TNF-α, IL-1β, and IL-6). Green tea polyphenols have a potential to be developed as radioprotective agents against irradiated-induced toxicity. Furthermore the antioxidant and anti-inflammatory activities of GTP can be attributed to the interaction of the different components through multiple and synergistic mechanisms.
authors.affiliation: Dept. of Clinical Pharmacology and Pharmacy, Centre of Pharmacy, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Hu
authors.forename: Dai-Hong
authors.initials: DH
authors.lastname: Guo
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Cao
authors.forename: Ya-Ping
authors.initials: YP
authors.lastname: Wang
authors.forename: Jian
authors.initials: J
authors.lastname: Yin
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhu
authors.forename: Khalid
authors.initials: K
authors.lastname: Rahman
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use; D000975:Antioxidants / Q000378:metabolism*; D002392:Catechin / Q000031:analogs & derivatives / Q000627:therapeutic use; D005720:Gamma Rays; D006401:Hematologic Agents / Q000627:therapeutic use; D053583:Interleukin-1beta / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D007970:Leukopenia / Q000188:drug therapy; D008315:Malondialdehyde / Q000097:blood; D051379:Mice; D015394:Molecular Structure; D059808:Polyphenols / Q000737:chemistry / Q000494:pharmacology*; D011832:Radiation Injuries / Q000188:drug therapy*; D011837:Radiation-Protective Agents / Q000627:therapeutic use; D013482:Superoxide Dismutase / Q000097:blood; D013662:Tea / Q000737:chemistry*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014916:Whole-Body Irradiation / Q000009:adverse effects*
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D006401:Hematologic Agents; D053583:Interleukin-1beta; D015850:Interleukin-6; D059808:Polyphenols; D011837:Radiation-Protective Agents; D013662:Tea; D014409:Tumor Necrosis Factor-alpha; D008315:Malondialdehyde; D002392:Catechin; C062669:epicatechin gallate; C045651:epigallocatechin gallate; D013482:Superoxide Dismutase; C057580:gallocatechol