title: Biosynthesis of 2-amino-2,3-dimethylbutyramide by nitrile hydratase from a newly isolated cyanide-resistant strain of Rhodococcus qingshengii.
abstract: Cells of a new isolate of Rhodococcus qingshengii harboring nitrile hydratase converted 2-amino-2,3-dimethylbutyronitrile into 2-amino-2,3-dimethylbutyramide (ADBA). Cells also hydrated a broad range of substrates including saturated, unsaturated and cyclic aliphatic nitriles. The microorganism tolerated KCN and ADBA up to 5 mM and 40 g/l, respectively. At 10 °C, ADBA reached 33.8 g/l (yield 84.5%) which was 2.5 times that at 30 °C.
authors.affiliation: Institute of Bioengineering, Zhijiang University of Technology, Hangzhou 310014, People's Republic of China.
authors.forename: Zhi-Jian
authors.initials: ZJ
authors.lastname: Lin
authors.forename: Ren-Chao
authors.initials: RC
authors.lastname: Zheng
authors.forename: Yu-Guo
authors.initials: YG
authors.lastname: Zheng
authors.forename: Yin-Chu
authors.initials: YC
authors.lastname: Shen
mesh_terms: D000577:Amides / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000378:metabolism; D001711:Biotransformation; D003486:Cyanides / Q000378:metabolism; D024881:Drug Resistance, Bacterial; D006836:Hydro-Lyases / Q000378:metabolism*; D012240:Rhodococcus / Q000187:drug effects / Q000201:enzymology*
chemical_list: D000577:Amides; D000900:Anti-Bacterial Agents; D003486:Cyanides; C006866:butyramide; D006836:Hydro-Lyases; C048831:nitrile hydratase