title: 1H, 13C and 15N resonance assignment of a 114-residue fragment of Engrailed 2 homeoprotein, a partially disordered protein.
abstract: Engrailed 2 is a transcription factor belonging to the class of homeoproteins. These proteins possess a 60-residue DNA binding globular domain and play an important role in the early stages of development. We expressed and purified a 13.4 kDa fragment of Engrailed 2, which comprises a 54-residue N-terminal extension in addition to the homeodomain region. Almost all backbone and side-chain resonances have been assigned. The weak dispersion in the proton dimension of the (1)H-(15)N HSQC spectrum indicates the presence of disordered regions that do not belong to the homeodomain. This work is a first step toward the NMR investigation of the structure and dynamics of Engrailed 2 protein that contains a well-structured globular domain and partially disordered regions.
authors.affiliation: UPMC Univ Paris 06, 4 Place Jussieu, 75252, Paris, Cedex 05, France.
authors.forename: Rafal
authors.initials: R
authors.lastname: Augustyniak
authors.forename: Stéphane
authors.initials: S
authors.lastname: Balayssac
authors.forename: Fabien
authors.initials: F
authors.lastname: Ferrage
authors.forename: Geoffrey
authors.initials: G
authors.lastname: Bodenhausen
authors.forename: Olivier
authors.initials: O
authors.lastname: Lequin
mesh_terms: D000818:Animals; D002645:Chickens; D018398:Homeodomain Proteins / Q000737:chemistry*; D007554:Isotopes / Q000737:chemistry; D009419:Nerve Tissue Proteins / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry; D000099266:Engrailed 2 Protein
chemical_list: D018398:Homeodomain Proteins; D007554:Isotopes; D009419:Nerve Tissue Proteins; D011994:Recombinant Proteins; D000099266:Engrailed 2 Protein