title: Establishment and characterization of canine rhabdomyosarcoma cell line CMS-C.
abstract: A novel canine tumor cell line designated as the CMS-C cell line was established from pleomorphic rhabdomyosarcoma (RMS) raised in the prostate gland of a 14-year-old intact male mixed-breed dog. CMS-C cells displayed the same immunohistochemical characteristics (positive for vimentin and desmin and negative for cytokeratin and smooth muscle actin) as the original tumor cells and express myoD1 and UCP3, known as striated muscle-specific molecules, as shown by RT-PCR assay. Therefore, the established CMS-C cell line appears to be of rhabdomyoblast cell origin. The CMS-C cell line established from pleomorphic RMS will be a useful tool for further studies about canine RMS.
authors.affiliation: Department of Veterinary Nursing, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, 1–7–1 Kyonan-cho, Musashino, Tokyo 180–8602, Japan. azakami@nvlu.ac.jp
authors.forename: Daigo
authors.initials: D
authors.lastname: Azakami
authors.forename: Hana
authors.initials: H
authors.lastname: Shibutani
authors.forename: Marie
authors.initials: M
authors.lastname: Dohi
authors.forename: Mariko
authors.initials: M
authors.lastname: Takasaki
authors.forename: Katsumi
authors.initials: K
authors.lastname: Ishioka
authors.forename: Akihiro
authors.initials: A
authors.lastname: Mori
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Momota
authors.forename: Makoto
authors.initials: M
authors.lastname: Bonkobara
authors.forename: Tsukimi
authors.initials: T
authors.lastname: Washizu
authors.forename: Masaki
authors.initials: M
authors.lastname: Michishita
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Hatakeyama
authors.forename: Seigo
authors.initials: S
authors.lastname: Ogasawara
authors.forename: Toshinori
authors.initials: T
authors.lastname: Sako
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor / Q000166:cytology; D004285:Dogs; D008297:Male; D011471:Prostatic Neoplasms / Q000473:pathology*; D012208:Rhabdomyosarcoma / Q000473:pathology*