title: Celiac disease and HLA typing using real-time PCR with melting curve analysis.
abstract: Celiac disease (CD) is an autoimmune disease characterized by chronic diarrhea, inflammatory lesions of small bowel and nutritional malabsorption. CD is strongly associated with the presence of HLA-DQB*02, DQB*03:02 and DQA*05. The absence of any one of these three human leukocyte antigen (HLA) alleles rules out the diagnosis of CD in suspected patients. Here, we describe a novel method to detect the presence of these specific HLA alleles using real-time polymerase chain reaction (PCR) with melting curve analysis. Compared with current HLA typing assays, the real-time PCR method is faster, requires fewer handling steps and provides 100% sensitivity and specificity for typing of HLA-DQB*02, DQB*03:02 and DQA*05 alleles.
authors.affiliation: Histocompatibility & Immunogenetics Laboratory, University of Utah School of Medicine, Salt Lake City, UT 84132-1904, USA. tracie.profaizer@hsc.utah.edu
authors.forename: T
authors.initials: T
authors.lastname: Profaizer
authors.forename: D
authors.initials: D
authors.lastname: Eckels
authors.forename: J C
authors.initials: JC
authors.lastname: Delgado
mesh_terms: D002446:Celiac Disease / Q000235:genetics* / Q000276:immunology; D003199:Computer Systems; D004247:DNA / Q000032:analysis; D004252:DNA Mutational Analysis / Q000379:methods; D006683:HLA-DQ Antigens / Q000032:analysis / Q000235:genetics; D059848:HLA-DQ alpha-Chains; D059866:HLA-DQ beta-Chains; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D009691:Nucleic Acid Denaturation / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013696:Temperature; D044366:Transition Temperature
chemical_list: D006683:HLA-DQ Antigens; D059848:HLA-DQ alpha-Chains; D059866:HLA-DQ beta-Chains; C069050:HLA-DQA1 antigen; C057615:HLA-DQbeta antigen; D004247:DNA