title: Architecture of the mammalian Golgi.
abstract: Since its first visualization in 1898, the Golgi has been a topic of intense morphological research. A typical mammalian Golgi consists of a pile of stapled cisternae, the Golgi stack, which is a key station for modification of newly synthesized proteins and lipids. Distinct stacks are interconnected by tubules to form the Golgi ribbon. At the entrance site of the Golgi, the cis-Golgi, vesicular tubular clusters (VTCs) form the intermediate between the endoplasmic reticulum and the Golgi stack. At the exit site of the Golgi, the trans-Golgi, the trans-Golgi network (TGN) is the major site of sorting proteins to distinct cellular locations. Golgi functioning can only be understood in light of its complex architecture, as was revealed by a range of distinct electron microscopy (EM) approaches. In this article, a general concept of mammalian Golgi architecture, including VTCs and the TGN, is described.
authors.affiliation: Department of Cell Biology, University Medical Center Utrecht, Heidelberglaan 100, 3584CX Utrecht, The Netherlands. j.klumperman@umcutrecht.nl
authors.forename: Judith
authors.initials: J
authors.lastname: Klumperman
mesh_terms: D000818:Animals; D022181:COP-Coated Vesicles / Q000502:physiology / Q000648:ultrastructure; D004721:Endoplasmic Reticulum / Q000648:ultrastructure; D006056:Golgi Apparatus / Q000502:physiology / Q000648:ultrastructure*; D006801:Humans; D008322:Mammals; D051379:Mice; D008854:Microscopy, Electron; D021601:trans-Golgi Network / Q000502:physiology / Q000648:ultrastructure