title: The sphingosine kinase activator K6PC-5 stimulates C2C12 myoblast differentiation.
abstract: Previously, K6PC-5, a synthetic derivative of ceramide, was demonstrated to activate sphingosine kinase (SK)-1 in keratinocytes. In this study its potential biological effect in mouse myoblasts was examined. The obtained results show that K6PC-5 promotes myogenic differentiation by enhancing myogenic marker expression, differentiation index and fusion index. Interestingly, its biological action was prevented by pharmacological inhibition of SK or S1P2 receptor, in full agreement with their recognized role in myoblast differentiation. This is the first evidence that pharmacological activation of SK accelerates myogenesis and suggests that this new therapeutic strategy could be possibly employed in skeletal muscle disorders where muscle regeneration is deficient.
authors.affiliation: Dipartimento di Scienze Biochimiche, Università di Firenze; Istituto Interuniversitario di Miologia, Firenze, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Bernacchioni
authors.forename: F
authors.initials: F
authors.lastname: Cencetti
authors.forename: M J
authors.initials: MJ
authors.lastname: Kwon
authors.forename: H S
authors.initials: HS
authors.lastname: Gwak
authors.forename: S K
authors.initials: SK
authors.lastname: Jeong
authors.forename: P
authors.initials: P
authors.lastname: Bruni
authors.forename: C
authors.initials: C
authors.lastname: Donati
mesh_terms: D000577:Amides / Q000494:pharmacology*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D004789:Enzyme Activation / Q000187:drug effects; D051379:Mice; D024510:Muscle Development / Q000187:drug effects*; D032446:Myoblasts / Q000166:cytology / Q000187:drug effects*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000502:physiology*; D049349:Receptors, Lysosphingolipid / Q000502:physiology; D000099157:Sphingosine Kinase
chemical_list: D000577:Amides; D017853:Phosphotransferases (Alcohol Group Acceptor); D049349:Receptors, Lysosphingolipid; D000099157:Sphingosine Kinase; C531172:N-(1,3-dihydroxyisopropyl)-2-hexyl-3-oxodecanamide