title: DA-9601 suppresses 2, 4-dinitrochlorobenzene and dust mite extract-induced atopic dermatitis-like skin lesions.
abstract: DA-9601 (Stillen™) is a novel anti-peptic formulation prepared from the ethanol extracts of Artemisia asiatica possessing anti-oxidative, anti-allergic and anti-inflammatory activities. However, their effect on atopic dermatitis (AD) has not been studied yet. In this study, we report that topical application of DA-9601 suppressed house dust mite extract (Dermatophagoides farinae extract, DFE) and 2, 4-dinitrochlorobenzene (DNCB)-induced AD-like skin lesions in BALB/c mice model. We established atopic dermatitis model in BALB/c mice by repeated local exposure of DFE/DNCB to the ears. Repeated alternative treatment of DFE/DNCB caused AD-like lesions. DA-9601 reduced AD-like skin lesions based on ear thickness and histopathological analysis, and serum IgE levels. DA-9601 inhibited mast cell infiltration into the ear and elevation of serum histamine in AD model. In addition, DA-9601 suppressed DFE/DNCB-induced expression of IL-4, IL-13, IL-31, and TNF-α in the ears. Taken together, our results showed that topical application of DA-9601 exerts beneficial effects in animal model of AD, suggesting that DA-9601 might be a candidate for the treatment of AD.
authors.affiliation: Division of Sport Science, College of Natural Sciences, Konkuk University, Chungbuk 380-702, Republic of Korea.
authors.forename: Eun-Ju
authors.initials: EJ
authors.lastname: Choi
authors.forename: Soyoung
authors.initials: S
authors.lastname: Lee
authors.forename: Ji-Sun
authors.initials: JS
authors.lastname: Hwang
authors.forename: Sin-Hyeog
authors.initials: SH
authors.lastname: Im
authors.forename: Chang-Duk
authors.initials: CD
authors.lastname: Jun
authors.forename: Hyun-Shik
authors.initials: HS
authors.lastname: Lee
authors.forename: Sang-Hyun
authors.initials: SH
authors.lastname: Kim
mesh_terms: D000287:Administration, Topical; D000818:Animals; D003876:Dermatitis, Atopic / Q000139:chemically induced / Q000188:drug therapy* / Q000276:immunology* / Q000473:pathology; D004137:Dinitrochlorobenzene / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D005260:Female; D006632:Histamine / Q000276:immunology / Q000378:metabolism; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D007378:Interleukins / Q000276:immunology / Q000378:metabolism; D008407:Mast Cells / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D010936:Plant Extracts / Q000494:pharmacology*; D039981:Pyroglyphidae / Q000737:chemistry / Q000276:immunology*; D012867:Skin / Q000187:drug effects* / Q000276:immunology* / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology / Q000378:metabolism
chemical_list: C116959:DA 9601; D004137:Dinitrochlorobenzene; D007378:Interleukins; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D007073:Immunoglobulin E; D006632:Histamine