title: Genetic analyses place most Spanish isolates of Beauveria bassiana in a molecular group with word-wide distribution.
abstract: BACKGROUND
  The entomopathogenic anamorphic fungus Beauveria bassiana is currently used as a biocontrol agent (BCA) of insects. Fifty-seven Beauveria bassiana isolates -53 from Spain- were characterized, integrating group I intron insertion patterns at the 3'-end of the nuclear large subunit ribosomal gene (LSU rDNA) and elongation factor 1-alpha (EF1-α) phylogenetic information, in order to assess the genetic structure and diversity of this Spanish collection of B. bassiana.
  
  
  RESULTS
  Group I intron genotype analysis was based on the four highly conserved insertion sites of the LSU (Ec2653, Ec2449, Ec2066, Ec1921). Of the 16 possible combinations/genotypes, only four were detected, two of which were predominant, containing 44 and 9 members out of 57 isolates, respectively. Interestingly, the members of the latter two genotypes showed unique differences in their growth temperatures. In follow, EF1-α phylogeny served to classify most of the strains in the B. bassiana s.s. (sensu stricto) group and separate them into 5 molecular subgroups, all of which contained a group I intron belonging to the IC1 subtype at the Ec1921 position. A number of parameters such as thermal growth or origin (host, geographic location and climatic conditions) were also examined but in general no association could be found.
  
  
  CONCLUSIONS
  Most Spanish B. bassiana isolates (77.2%) are grouped into a major phylogenetic subgroup with word-wide distribution. However, high phylogenetic diversity was also detected among Spanish isolates from close geographic zones with low climatic variation. In general, no correlation was observed between the molecular distribution and geographic origin or climatic characteristics where the Spanish B. bassiana isolates were sampled.
authors.affiliation: Centro Hispano-Luso de Investigaciones Agrarias (CIALE), Departamento de Microbiología y Genética, Universidad de Salamanca, Río Duero 12, Campus de Villamayor, 37185 Salamanca, Spain.
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Garrido-Jurado
authors.forename: Marcela
authors.initials: M
authors.lastname: Márquez
authors.forename: Almudena
authors.initials: A
authors.lastname: Ortiz-Urquiza
authors.forename: Cándido
authors.initials: C
authors.lastname: Santiago-Álvarez
authors.forename: Enrique A
authors.initials: EA
authors.lastname: Iturriaga
authors.forename: Enrique
authors.initials: E
authors.lastname: Quesada-Moraga
authors.forename: Enrique
authors.initials: E
authors.lastname: Monte
authors.forename: Rosa
authors.initials: R
authors.lastname: Hermosa
mesh_terms: D000818:Animals; D052982:Beauveria / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016000:Cluster Analysis; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D020459:Genes, rRNA; D007313:Insecta / Q000382:microbiology*; D007438:Introns; D008969:Molecular Sequence Data; D020648:Peptide Elongation Factor 1 / Q000235:genetics*; D010802:Phylogeny; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D013030:Spain
chemical_list: D004271:DNA, Fungal; D020648:Peptide Elongation Factor 1