title: Biochemical and biophysical characterization of collagens of marine sponge, Ircinia fusca (Porifera: Demospongiae: Irciniidae).
abstract: Collagens were isolated and partially characterized from the marine demosponge, Ircinia fusca from Gulf of Mannar (GoM), India, with an aim to develop potentially applicable collagens from unused and under-used resources. The yield of insoluble, salt soluble and acid soluble forms of collagens was 31.71 ± 1.59, 20.69 ± 1.03, and 17.38 ± 0.87 mg/g dry weight, respectively. Trichrome staining, Scanning & Transmission Electron microscopic (SEM & TEM) studies confirmed the presence of collagen in the isolated, terminally globular irciniid filaments. The partially purified (gel filtration chromatography), non-fibrillar collagens appeared as basement type collagenous sheets under light microscopy whereas the purified fibrillar collagens appeared as fibrils with a repeated band periodicity of 67 nm under Atomic Force Microscope (AFM). The non-fibrillar and fibrillar collagens were seen to have affinity for anti-collagen type IV and type I antibodies raised against human collagens, respectively. The macromolecules, i.e., total protein, carbohydrate and lipid contents within the tissues were also quantified. The present information on the three characteristic irciniid collagens (filamentous, fibrillar and non-fibrillar) could assist the future attempts to unravel the therapeutically important, safer collagens from marine sponges for their use in pharmaceutical and cosmeceutical industries.
authors.affiliation: Toxicology Unit, Biology Division, Indian Institute of Chemical Technology, Hyderabad 500 607, India.
authors.forename: Ramjee
authors.initials: R
authors.lastname: Pallela
authors.forename: Sreedhar
authors.initials: S
authors.lastname: Bojja
authors.forename: Venkateswara Rao
authors.initials: VR
authors.lastname: Janapala
mesh_terms: D000818:Animals; D002850:Chromatography, Gel; D003094:Collagen / Q000302:isolation & purification* / Q000648:ultrastructure*; D007194:India; D018625:Microscopy, Atomic Force; D008854:Microscopy, Electron; D011161:Porifera / Q000033:anatomy & histology / Q000737:chemistry*
chemical_list: D003094:Collagen