title: Biodegradable glutaraldehyde-crosslinked casein conduit promotes regeneration after peripheral nerve injury in adult rats.
abstract: In this study, GCC protein was used for the first time to construct a biodegradable conduit for peripheral nerve repair. The GCC was highly stable with a sufficiently high level of mechanical properties and it was non-toxic and non-apoptotic which could maintain the survival and outgrowth of Schwann cells. Noninvasive bioluminescence imaging accompanied with histochemical assessment showed the GCC was highly biocompatible after subcutaneous implantation in transgenic mice. Electrophysiology, labeling of calcitonin gene-related peptide in the lumbar spinal cord and histology analysis also showed a rapid morphological and functional recovery for disrupted rat sciatic nerves repaired with the GCC conduits. Therefore, we conclude that the GCC can offer great nerve regeneration characteristics and can be a promising material for the successful repair of peripheral nerve defects.
authors.affiliation: Laboratory of Biomaterials, School of Chinese Medicine, China Medical University, Taichung, Taiwan.
authors.forename: Walter
authors.initials: W
authors.lastname: Wang
authors.forename: Jia-Horng
authors.initials: JH
authors.lastname: Lin
authors.forename: Chin-Chuan
authors.initials: CC
authors.lastname: Tsai
authors.forename: Hao-Che
authors.initials: HC
authors.lastname: Chuang
authors.forename: Chien-Yi
authors.initials: CY
authors.lastname: Ho
authors.forename: Chun-Hsu
authors.initials: CH
authors.lastname: Yao
authors.forename: Yueh-Sheng
authors.initials: YS
authors.lastname: Chen
mesh_terms: D000818:Animals; D002116:Calcitonin / Q000235:genetics; D002364:Caseins / Q000737:chemistry / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D004594:Electrophysiology; D005976:Glutaral / Q000737:chemistry / Q000494:pharmacology*; D048091:Guided Tissue Regeneration; D008160:Lumbosacral Plexus; D008164:Luminescent Proteins / Q000032:analysis; D051379:Mice; D008822:Mice, Transgenic; D009416:Nerve Regeneration / Q000187:drug effects* / Q000502:physiology; D059348:Peripheral Nerve Injuries; D010525:Peripheral Nerves / Q000187:drug effects / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000166:cytology / Q000187:drug effects; D012584:Sciatic Nerve / Q000187:drug effects* / Q000293:injuries / Q000473:pathology; D020196:Trauma, Nervous System / Q000473:pathology
chemical_list: D002364:Caseins; D008164:Luminescent Proteins; D002116:Calcitonin; D005976:Glutaral