title: Production of influenza pseudotyped lentiviral particles and their use in influenza research and diagnosis: an update.
abstract: Pseudotyped viral particles are being used as safe surrogates to mimic the structure and surface of many viruses, including highly pathogenic viruses such as avian influenza H5N1, to investigate biological functions mediated by the envelope proteins derived from these viruses. The first part of this article evaluates and discusses the differences in the production and characterization of influenza pseudoparticles. The second part focuses on the applications that such a flexible tool can provide in modern influenza research, in particular in the fields of drug discovery, molecular biology and diagnosis.
authors.affiliation: HKU-Pasteur Research Centre, Dexter HC Man Building, 8 Sassoon Road, Pokfulam, Hong Kong. jmgarcia@hku.hk
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Garcia
authors.forename: Jimmy C C
authors.initials: JC
authors.lastname: Lai
mesh_terms: D000818:Animals; D001715:Bird Diseases / Q000175:diagnosis / Q000821:virology; D001717:Birds; D002678:Chimera / Q000502:physiology; D055808:Drug Discovery; D006801:Humans; D005585:Influenza in Birds / Q000175:diagnosis / Q000821:virology; D007251:Influenza, Human / Q000175:diagnosis / Q000821:virology; D029521:Alphainfluenzavirus / Q000502:physiology*; D016086:Lentivirus / Q000235:genetics* / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D008967:Molecular Biology; D009976:Orthomyxoviridae Infections / Q000175:diagnosis* / Q000821:virology; D010957:Plasmids / Q000502:physiology; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis / Q000821:virology; D014162:Transfection; D014764:Viral Proteins / Q000378:metabolism*; D014771:Virion / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D008562:Membrane Glycoproteins; D014764:Viral Proteins