title: Conformal nano-thin modified polyelectrolyte coatings for encapsulation of cells.
abstract: Encapsulation of cells in polymeric shells allows for separation of biological material from produced factors, which may find biotechnological and biomedical applications. Human T-lymphocyte cell line Jurkat as well as rat pancreatic islets were encapsulated using LbL technique within shells of polyelectrolyte modified by incorporation of biotin complexed with avidin to improve cell coating and to create the potential ability to elicit specific biochemical responses. The coating with nano-thin modified shells allowed for maintenance of the evaluated cells' integrity and viability during the 8-day culture. The different PE impact may be observed on different biological materials. The islets exhibited lower mitochondrial activity than the Jurkat cells. Nevertheless, coating of cells with polyelectrolyte modified membrane allowed for functioning of both model cell types: 10 μm leukemia cells or 150 μm islets during the culture. Applied membranes maintained the molecular structure during the culture period. The conclusion is that applied modified membrane conformation may be recommended for coating shells for biomedical purposes.
authors.affiliation: Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Science, Warsaw, Poland. ludomira@ibib.waw.pl
authors.forename: L H
authors.initials: LH
authors.lastname: Granicka
authors.forename: M
authors.initials: M
authors.lastname: Antosiak-Iwańska
authors.forename: E
authors.initials: E
authors.lastname: Godlewska
authors.forename: M
authors.initials: M
authors.lastname: Strawski
authors.forename: M
authors.initials: M
authors.lastname: Szklarczyk
authors.forename: B
authors.initials: B
authors.lastname: Maranowski
authors.forename: C
authors.initials: C
authors.lastname: Kowalewski
authors.forename: J
authors.initials: J
authors.lastname: Wiśniewsk
mesh_terms: D000818:Animals; D001360:Avidin / Q000737:chemistry / Q000378:metabolism; D001709:Biotechnology / Q000379:methods*; D001710:Biotin / Q000737:chemistry / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D018914:Cells, Immobilized / Q000166:cytology / Q000378:metabolism*; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology; D005453:Fluorescence; D006801:Humans; D007515:Islets of Langerhans / Q000166:cytology / Q000378:metabolism*; D019169:Jurkat Cells / Q000166:cytology / Q000378:metabolism*; D018613:Microscopy, Confocal; D049329:Nanostructures / Q000737:chemistry; D011094:Polyethyleneimine / Q000737:chemistry; D011107:Polylysine / Q000737:chemistry; D051381:Rats
chemical_list: D020099:Coated Materials, Biocompatible; D001360:Avidin; D011107:Polylysine; D001710:Biotin; D011094:Polyethyleneimine