title: Streptomyces qinglanensis sp. nov., isolated from mangrove sediment.
abstract: A Streptomyces-like strain, 172205(T), was obtained from mangrove soil collected at Qinglan Harbour, Wenchang, Hainan, China. The strain was characterized by white aerial mycelium and long spore chains. Comparison of 16S rRNA gene sequences indicated that the strain represents a novel member of the genus Streptomyces, exhibiting highest levels of similarity (<98.29%) to the type strains of members of the genus Streptomyces. However, DNA-DNA relatedness and phenotypic data readily distinguished strain 172205(T) from phylogenetically related type strains. The predominant menaquinones were MK-9(H(6)) and MK-9(H(8)). The major fatty acids were iso-C(15:0) (10.31%), anteiso-C(15:0) (35.19%), iso-C(16:0) (20.24%) and anteiso-C(17:0) (10.05%). The diagnostic phospholipid was phosphatidylethanolamine. The cell wall contained ll-diaminopimelic acid and meso-diaminopimelic acid and whole-cell hydrolysates contained ribose, galactose and glucose. The results of DNA-DNA hybridization, physiological and biochemical tests allowed the genotypic and phenotypic differentiation of strain 172205(T) from phylogenetically related type strains. Therefore, strain 172205(T) is considered to represent a novel species of the genus Streptomyces, for which the name Streptomyces qinglanensis sp. nov. is proposed. The type strain is 172205(T) (=CGMCC 4.6825(T) =DSM 42035(T)).
authors.affiliation: College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.|Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agriculture Sciences, Haikou 571101, PR China.
authors.forename: Huo
authors.initials: H
authors.lastname: Hu
authors.affiliation: Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agriculture Sciences, Haikou 571101, PR China.
authors.forename: Hai-Peng
authors.initials: HP
authors.lastname: Lin
authors.affiliation: Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agriculture Sciences, Haikou 571101, PR China.
authors.forename: Qingyi
authors.initials: Q
authors.lastname: Xie
authors.affiliation: Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agriculture Sciences, Haikou 571101, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.affiliation: Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agriculture Sciences, Haikou 571101, PR China.
authors.forename: Xin-Qiang
authors.initials: XQ
authors.lastname: Xie
authors.affiliation: Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, and Wuhan University School of Pharmaceutical Sciences, Wuhan 430071, PR China.|College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.|Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agriculture Sciences, Haikou 571101, PR China.
authors.forename: Kui
authors.initials: K
authors.lastname: Hong
mesh_terms: D015373:Bacterial Typing Techniques; D002473:Cell Wall / Q000737:chemistry; D002681:China; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003960:Diaminopimelic Acid / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013171:Spores, Bacterial / Q000166:cytology; D013302:Streptomyces / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D024482:Vitamin K 2 / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D003960:Diaminopimelic Acid