title: Ecofriendly degradation, decolorization and detoxification of textile effluent by a developed bacterial consortium.
abstract: Present study illustrates the effectual decolorization and degradation of the textile effluent using a developed bacterial consortium SDS, consisted of bacterial species Providencia sp. SDS and Pseudomonas aeuroginosa strain BCH, originally isolated from dye contaminated soil. The intensive metabolic activity of the consortium SDS led to complete decolorization of textile effluent within 20 h at pH 7 and temperature 30°C. Significant induction in the activities of veratryl alcohol oxidase, laccase, azoreductase and DCIP reductase were observed during decolorization, which indicates their involvement in decolorization and degradation process. The decolorization and biodegradation was monitored using UV-vis spectroscopy, IR spectroscopy, HPLC and HPTLC analysis. Toxicological analysis of effluent before and after treatment was performed using classical Allium cepa test. Investigations of various toxicological parameters viz, oxidative stress response, cytotoxicity, genotoxicity and phytotoxicity, collectively concludes that, the toxicity of effluent reduces significantly after treatment with consortium SDS.
authors.affiliation: Department of Biochemistry, Shivaji University, Kolhapur 416004, India.
authors.forename: Swapnil S
authors.initials: SS
authors.lastname: Phugare
authors.forename: Dayanand C
authors.initials: DC
authors.lastname: Kalyani
authors.forename: Shripad N
authors.initials: SN
authors.lastname: Surwase
authors.forename: Jyoti P
authors.initials: JP
authors.lastname: Jadhav
mesh_terms: D000429:Alcohol Oxidoreductases / Q000378:metabolism; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002851:Chromatography, High Pressure Liquid; D004396:Coloring Agents / Q000032:analysis / Q000378:metabolism* / Q000633:toxicity; D042845:Laccase / Q000378:metabolism; D059013:Microbial Consortia; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D009601:Nitroreductases; D019697:Onions / Q000187:drug effects; D011532:Providencia / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D011549:Pseudomonas / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D011808:Quinone Reductases; D013783:Textile Industry; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000378:metabolism* / Q000633:toxicity
chemical_list: D004396:Coloring Agents; D014874:Water Pollutants, Chemical; D000429:Alcohol Oxidoreductases; C057990:veratryl alcohol oxidase; D042845:Laccase; D009247:NADH, NADPH Oxidoreductases; D011808:Quinone Reductases; C033633:dichlorophenolindophenol reductase; D009601:Nitroreductases; C021106:azoreductase