title: Molecular genotyping and epidemiology of Mycobacterium bovis strains obtained from cattle in Iran.
abstract: Restriction fragment length polymorphism (RFLP) genotyping was employed to analyze the population genetics of Mycobacterium bovis in Iran. One hundred and twenty-three isolates collected from slaughtered tuberculosis-suspect cattle and one clinically asymptomatic buffalo were subjected to RFLP analysis with probes of the polymorphic GC-rich sequence (PGRS) and the direct repeat sequence (DR) using DNA digested with PvuII and AluI. All these methods detected a large homogeneous population in which only a few isolates had variant genotypes. Only AluI-based RFLPs of both the PGRS and DR sequences were able to clearly differentiate between BCG and field strains of M. bovis. As in previous reports, these findings seem to reflect a recent dispersal of one or a few strains in Iran following the substantial expansion of Holstein-Friesian cattle over the last few decades.
authors.affiliation: PPD Tuberculin Department, Razi Vaccine & Serum Research Institute, Karaj, Iran.
authors.forename: Nader
authors.initials: N
authors.lastname: Mosavari
authors.forename: Mohammad Mehdi
authors.initials: MM
authors.lastname: Feizabadi
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Jamshidian
authors.forename: Masoud Reza Seifiabadi
authors.initials: MR
authors.lastname: Shahpouri
authors.forename: Ken J
authors.initials: KJ
authors.lastname: Forbes
authors.forename: Reza Aref
authors.initials: RA
authors.lastname: Pajoohi
authors.forename: Rouholah
authors.initials: R
authors.lastname: Keshavarz
authors.forename: Mohammad Mohammad
authors.initials: MM
authors.lastname: Taheri
authors.forename: Keyvan
authors.initials: K
authors.lastname: Tadayon
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D002020:Buffaloes / Q000382:microbiology; D002417:Cattle / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D005828:Genetics, Population; D005838:Genotype; D007492:Iran / Q000453:epidemiology; D017720:Molecular Epidemiology; D009163:Mycobacterium bovis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012091:Repetitive Sequences, Nucleic Acid; D014380:Tuberculosis, Bovine / Q000453:epidemiology* / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial