title: Genetic variation in the promoter of DNMT3B is associated with the risk of colorectal cancer.
abstract: OBJECTIVE
  DNA methyltransferase-3B (DNMT3B) plays an important role in the generation of aberrant methylation in carcinogenesis. Polymorphisms of the DNMT3B gene may influence DNMT3B enzyme activity on DNA methylation, thereby modulating the susceptibility to colorectal cancer (CRC).
  
  
  METHODS
  The polymorphisms in the promoter region of the DNMT3B gene [-149C>T (rs2424913) and -579G>T (rs1569686)] were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), and a total of 544 CRC patients and 533 age- and sex-matched healthy controls were enrolled in the case-control study.
  
  
  RESULTS
  The results showed that the -579G allele was associated with a significantly decreased risk of CRC (adjusted OR, 0.50; 95%CI, 0.35-0.72; P = 0.0002) when compared with the -579TT genotype. However, the DNMT3B-149CT genotype was not associated with the risk of CRC (adjusted OR, 0.48; 95%CI, 0.18-1.30; P = 0.151). In addition, stratification analysis revealed that the increased risk was predominant in both colon cancer and rectal cancer showing no effect of primary occurrence site.
  
  
  CONCLUSIONS
  Our research demonstrated the -579G allele was a potential protective factor for the occurrence of CRC.
authors.affiliation: Department of Life Sciences, Nanjing Normal University, Nanjing, 210046, Jiangsu Province, China. Baoqian001@126.com
authors.forename: Qian
authors.initials: Q
authors.lastname: Bao
authors.forename: Bangshun
authors.initials: B
authors.lastname: He
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Pan
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Tang
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Lili
authors.initials: L
authors.lastname: Qu
authors.forename: Yongfei
authors.initials: Y
authors.lastname: Xu
authors.forename: Chan
authors.initials: C
authors.lastname: Zhu
authors.forename: Fuliang
authors.initials: F
authors.lastname: Tian
authors.forename: Shukui
authors.initials: S
authors.lastname: Wang
mesh_terms: D016022:Case-Control Studies; D015179:Colorectal Neoplasms / Q000201:enzymology* / Q000235:genetics*; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000235:genetics*; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D014644:Genetic Variation; D006801:Humans; D008297:Male; D008875:Middle Aged; D059645:Mutation Rate; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012307:Risk Factors; D000097584:DNA Methyltransferase 3B
chemical_list: D004248:DNA (Cytosine-5-)-Methyltransferases; D000097584:DNA Methyltransferase 3B