title: Functional properties of suburothelial microvessels in the rat bladder.
abstract: OBJECTIVE
  We investigated the properties of suburothelial microvessels, which have a vital role in maintaining microcirculation to cells involved in bladder afferent signaling.
  
  
  METHODS
  Changes in the diameter of rat bladder suburothelial microvessels were measured using video microscopy. Membrane potential changes and intracellular Ca(2+) dynamics of suburothelial venules were examined using intracellular recording techniques and Ca(2+) imaging of fluo-4 fluorescence, respectively.
  
  
  RESULTS
  Suburothelial venules showed spontaneous action potential and vasoconstriction activity while suburothelial arterioles were quiescent. Venular vasoconstriction was prevented by cyclopiazonic acid or nicardipine and decreased by 2-aminoethoxydiphenyl borate, niflumic acid or 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid. Venular smooth muscle cells and perivascular interstitial cells showed spontaneous Ca(2+) transients. Nicardipine decreased the amplitude and disrupted the synchronicity of Ca(2+) transients in and between the 2 cell populations. Residual Ca(2+) transients in nicardipine occurred asynchronously and were abolished by cyclopiazonic acid. Suburothelial arterioles constricted in response to transmural nerve stimulation. These nerve induced constrictions were suppressed by prazosin or the selective α(1A) blocker RS17053 but not by the α(1D) blocker BMY7378. Remaining constrictions were abolished by guanethidine.
  
  
  CONCLUSIONS
  Spontaneous vasoconstriction of suburothelial venules appears to result upon Ca(2+) release from the sarcoplasmic reticulum upon activation of inositol 1,4,5-trisphosphate receptors. This Ca(2+) opens Ca(2+) activated Cl(-) channels to trigger action potentials and Ca(2+) influx through L-type Ca(2+) channels. Adjacent perivascular interstitial cells may also have a role in generating this spontaneous venular vasoconstriction. In contrast, sympathetic nerve released noradrenaline acts on α(1A)-adrenoceptors to constrict suburothelial arterioles.
authors.affiliation: Department of Cell Physiology, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan. hasitani@med.nagoya-cu.ac.jp
authors.forename: Hikaru
authors.initials: H
authors.lastname: Hashitani
authors.forename: Hiromich
authors.initials: H
authors.lastname: Takano
authors.forename: Kohei
authors.initials: K
authors.lastname: Fujita
authors.forename: Retsu
authors.initials: R
authors.lastname: Mitsui
authors.forename: Hikaru
authors.initials: H
authors.lastname: Suzuki
mesh_terms: D000818:Animals; D020013:Calcium Signaling; D055724:Electrophysiological Phenomena; D008297:Male; D055806:Microvessels / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D001743:Urinary Bladder / Q000098:blood supply*; D019459:Urothelium / Q000098:blood supply*