title: pGOODs: new plasmids for the co-expression of proteins in Escherichia coli.
abstract: Two systems for the co-expression of proteins in Escherichia coli were designed and constructed. The first system relies on the new vector, pGOOD, which is compatible with ColE1-type plasmids and sustains efficient co-expression of soluble protein complexes. The second system is based on the pGOOD1 vector (a derivative of pGOOD), useful for the production of toxic proteins, whose synthesis can be regulated by the co-expressed LacI repressor.
authors.affiliation: Department of Industrial Chemistry, University of Bologna, Viale Risorgimento 4, 40136 Bologna, Italy.
authors.forename: Emanuele
authors.initials: E
authors.lastname: Conte
authors.forename: Gianpiero
authors.initials: G
authors.lastname: Landolfi
authors.forename: Gabriele
authors.initials: G
authors.lastname: Vincelli
authors.forename: Alessandra
authors.initials: A
authors.lastname: Stefan
authors.forename: Alejandro
authors.initials: A
authors.lastname: Hochkoeppler
mesh_terms: D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000096:biosynthesis* / Q000235:genetics*; D015870:Gene Expression; D005818:Genetic Engineering / Q000379:methods; D005822:Genetic Vectors; D010957:Plasmids; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics
chemical_list: D029968:Escherichia coli Proteins; D011994:Recombinant Proteins