title: ODV-associated proteins of the Pieris rapae granulovirus.
abstract: Alphabaculovirus (lepidopteran-specific nucleopolyhedroviruses, NPV) and Betabaculovirus (granuloviruses, GV) are two main genera of the family Baculoviridae. The virion proteomes of Alphabaculovirus have been well studied; however, the Betabaculovirus virion compositions remain unclear. Pieris rapae granulovirus (PrGV) can kill larvae of P. rapae, a worldwide and important pest of mustard family crops. In this study, the occlusion-derived virus (ODV)-associated proteins of PrGV were identified using three mass spectrometry (MS) approaches. The MS analyses demonstrated that 47 proteins were present in PrGV-ODV. Of the 47 PrGV-ODV proteins, 33 have homologues identified previously in other baculovirus ODV/BVs, whereas 14 (P10, Pr21, Pr29, Pr35, Pr42, Pr54, P45/48, Pr83, Pr84, Pr89, Pr92, Pr111, Pr114 and FGF3) were newly identified ODV proteins. Seven of the 14 newly identified ODV proteins are specific to Betabaculovirus, including Pr35, Pr42, Pr54, Pr83, Pr84, Pr111 and Pr114. Furthermore, the data derived from these MS approaches were validated by immunoblotting analysis using antisera prepared from 11 randomly selected recombinant PrGV-ODV proteins (including 5 Betabaculovirus-unique proteins). Comparison analyses revealed the similar and different compositions between Betabaculovirus and Alphabaculovirus virions, which deepen our understanding of the baculovirus virion structure and provide helpful information on Betabaculovirus--host interaction studies.
authors.affiliation: Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture, Institute of Insect Science, Zhejiang-California International Nanosystem Institute, Zhejiang University, Hangzhou 310058, China.
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Wang
authors.forename: Bao-Qin
authors.initials: BQ
authors.lastname: Zhang
authors.forename: Hai-Jun
authors.initials: HJ
authors.lastname: Xu
authors.forename: Ying-Jun
authors.initials: YJ
authors.lastname: Cui
authors.forename: Yi-Peng
authors.initials: YP
authors.lastname: Xu
authors.forename: Min-Juan
authors.initials: MJ
authors.lastname: Zhang
authors.forename: Yeon Soo
authors.initials: YS
authors.lastname: Han
authors.forename: Yong Seok
authors.initials: YS
authors.lastname: Lee
authors.forename: Yan-Yuan
authors.initials: YY
authors.lastname: Bao
authors.forename: Chuan-Xi
authors.initials: CX
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D002080:Butterflies / Q000821:virology*; D017124:Conserved Sequence; D025722:Granulovirus / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D007106:Immune Sera; D007181:Inclusion Bodies, Viral / Q000378:metabolism* / Q000648:ultrastructure; D008970:Molecular Weight; D010572:Pest Control, Biological; D020543:Proteome / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D007106:Immune Sera; D020543:Proteome; D011994:Recombinant Proteins; D014764:Viral Proteins