title: Trehalose has a protective effect on human brain-type creatine kinase during thermal denaturation.
abstract: We investigated the effects of trehalose on thermal inactivation and aggregation of human brain-type creatine kinase (hBBCK) in this study. In the presence of 1.0 M trehalose, the midpoint temperature of thermal inactivation (T (m)) of hBBCK increased by 4.6 °C, and the activation energy (E (a)) for thermal inactivation increased from 29.7 to 41.1 kJ mol(-1). Intrinsic fluorescence spectra also showed an increase in the apparent transition temperature (T (1/2)) of hBBCK from 43.0 °C to 46.5 °C, 47.7 °C, and 49.9 °C in 0, 0.6, 0.8, and 1.2 M trehalose, respectively. In addition, trehalose significantly blocked the aggregation of hBBCK during thermal denaturation. Our results indicate that trehalose has potential applications as a thermal stabilizer and may aid in the folding of other enzymes in addition to hBBCK.
authors.affiliation: School of Life Science, Ningxia University, Yinchuan 750021, People's Republic of China.
authors.forename: Jiang-Liu
authors.initials: JL
authors.lastname: Yang
authors.forename: Hang
authors.initials: H
authors.lastname: Mu
authors.forename: Zhi-Rong
authors.initials: ZR
authors.lastname: Lü
authors.forename: Shang-Jun
authors.initials: SJ
authors.lastname: Yin
authors.forename: Yue-Xiu
authors.initials: YX
authors.lastname: Si
authors.forename: Sheng-Mei
authors.initials: SM
authors.lastname: Zhou
authors.forename: Fang
authors.initials: F
authors.lastname: Zhang
authors.forename: Wei-Jiang
authors.initials: WJ
authors.lastname: Hu
authors.forename: Fan-Guo
authors.initials: FG
authors.lastname: Meng
authors.forename: Hai-Meng
authors.initials: HM
authors.lastname: Zhou
authors.forename: Zi-Ping
authors.initials: ZP
authors.lastname: Zhang
authors.forename: Guo-Ying
authors.initials: GY
authors.lastname: Qian
mesh_terms: D055731:Carbohydrate Biochemistry / Q000379:methods*; D052276:Creatine Kinase, BB Form / Q000737:chemistry / Q000378:metabolism*; D004795:Enzyme Stability / Q000187:drug effects; D006358:Hot Temperature; D006801:Humans; D007700:Kinetics; D011489:Protein Denaturation / Q000187:drug effects*; D017510:Protein Folding / Q000187:drug effects; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics; D014199:Trehalose / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D014199:Trehalose; D052276:Creatine Kinase, BB Form