title: [Cell continuous extraction-HPLC determination biological affinity of 8 bufadienolides on MGC-803 cells and their correlation with anti-tumor activities].
abstract: OBJECTIVE
  To study the bioaffinity between 8 bufadienolides(Bu) and tumor cells and analyze the correlation between the bioaffinity and the anti-tumor activities of Bu.
  
  
  METHODS
  Mix and cultivate the chloroform extract of Chansu and MGC-803. Measure the content of 8 Bu in supernatant and cells using HPLC and calculate their affinity rate.
  
  
  RESULTS
  The coefficient correlation between the decrease of Bu in cell supernatant after affinity and its MGC-803 restrictive activities, and between the cotent percentage of the free Bu in free cells with its MGC-803 restrictive activities, and between the difference between the decrease and the percentage and its MGC-803 restrictive activities is r = 0.82 (P < 0.05), r = -0.04 and r = 0.83 (P < 0.05) respectively.
  
  
  CONCLUSIONS
  Eight Bu have different levels of affinity with MGC-803 which correlate with their anti-tumor activities.
authors.affiliation: Jiangsu Key Laboratory for Traditional Chinese Medicine Formulae Research, Nanjing University of Traditional Chinese Medicine, Nanjing 210046, China. jiangjj_118@126.com
authors.forename: Jiejun
authors.initials: J
authors.lastname: Jiang
authors.forename: Fenqiang
authors.initials: F
authors.lastname: You
authors.forename: Hongyue
authors.initials: H
authors.lastname: Ma
authors.forename: Jing
authors.initials: J
authors.lastname: Zhong
authors.forename: Junfeng
authors.initials: J
authors.lastname: Zhang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhan
authors.forename: Yuping
authors.initials: Y
authors.lastname: Tang
authors.forename: Jin'ao
authors.initials: J
authors.lastname: Duan
authors.forename: Anwei
authors.initials: A
authors.lastname: Ding
mesh_terms: D000664:Amphibian Venoms / Q000737:chemistry*; D000818:Animals; D000970:Antineoplastic Agents / Q000302:isolation & purification* / Q000494:pharmacology*; D001001:Anura; D002018:Bufanolides / Q000302:isolation & purification* / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D009369:Neoplasms / Q000188:drug therapy*
chemical_list: D000664:Amphibian Venoms; D000970:Antineoplastic Agents; D002018:Bufanolides; C095591:chan su