title: Partial protection against classical swine fever virus elicited by dendrimeric vaccine-candidate peptides in domestic pigs.
abstract: We report the immunogenicity of three dendrimeric peptide vaccine candidates for classical swine fever virus (CSFV). Each dendrimeric construct contained four copies of a B-cell epitope from the E2 glycoprotein of CSFV [construct 1: E2 (694-712); 2: E2 (712-727); 3: E2 (829-842)] joined to a T-cell epitope from the NS3 protein (residues 1446-1460). Intramuscular immunization of domestic pigs with the different constructs significantly reduced the clinical score after lethal challenge with CSFV. In contrast, control pigs developed severe clinical signs of the disease. All pigs vaccinated with construct 1, containing a B-cell epitope from the E2 B-C domain, developed an antibody response that recognized not only the original dendrimeric immunogen but also its constituting E2 epitope in linear form, albeit no neutralizing antibodies were detected prior to viral challenge. Two of these pigs were partially protected, which associated with the induction of IFN-γ producing cells and of neutralizing antibodies upon challenge. Interestingly, the serological response elicited by construct 1 lacked antibodies to E2 A domain, used as infection markers. The dendrimeric approach could therefore provide a basis for the development of CSFV marker (DIVA) vaccines, and contribute to a better understanding of the immune responses against CSFV.
authors.affiliation: Centre de Recerca en Sanitat Animal, IRTA-UAB, Campus de UAB, 08193 Bellaterra, Barcelona, Spain.
authors.forename: Joan
authors.initials: J
authors.lastname: Tarradas
authors.forename: Marta
authors.initials: M
authors.lastname: Monsó
authors.forename: Marta
authors.initials: M
authors.lastname: Muñoz
authors.forename: Rosa
authors.initials: R
authors.lastname: Rosell
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Fraile
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Frías
authors.forename: Mariano
authors.initials: M
authors.lastname: Domingo
authors.forename: David
authors.initials: D
authors.lastname: Andreu
authors.forename: Francisco
authors.initials: F
authors.lastname: Sobrino
authors.forename: Llilianne
authors.initials: L
authors.lastname: Ganges
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D002460:Cell Line; D006691:Classical Swine Fever / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D006692:Classical Swine Fever Virus / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism / Q000472:pathogenicity; D050091:Dendrimers / Q000008:administration & dosage* / Q000138:chemical synthesis / Q000737:chemistry; D018985:Epitopes, B-Lymphocyte / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology; D018984:Epitopes, T-Lymphocyte / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology; D007114:Immunization; D007371:Interferon-gamma / Q000096:biosynthesis; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D010455:Peptides / Q000008:administration & dosage* / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology; D020365:RNA Helicases / Q000737:chemistry / Q000276:immunology; D012697:Serine Endopeptidases / Q000737:chemistry / Q000276:immunology; D034421:Sus scrofa; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000276:immunology; D017361:Viral Nonstructural Proteins / Q000737:chemistry / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage* / Q000737:chemistry / Q000276:immunology; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D050091:Dendrimers; D018985:Epitopes, B-Lymphocyte; D018984:Epitopes, T-Lymphocyte; D007371:Interferon-gamma; D010455:Peptides; D020365:RNA Helicases; D012697:Serine Endopeptidases; D014759:Viral Envelope Proteins; D017361:Viral Nonstructural Proteins; D014765:Viral Vaccines; C060028:NS3 protein, flavivirus; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases; C087086:glycoprotein E2, classical swine fever virus