title: Neuroprotective effect of magnesium sulfate treatment on fetal brain in experimental intrauterine ischemia reperfusion injury.
abstract: OBJECTIVE
  The aim of this study was to demonstrate the neuroprotective effect of magnesium sulfate on ischemia-reperfusion-induced injury in fetal rat brain.
  
  
  METHODS
  Twenty-four, 19-days pregnant rats were randomly allocated into four groups. Fetal brain ischemia was induced by clamping the utero-ovarian artery bilaterally for 20 min and reperfusion was achieved by removing the clamps for 30 min. The control group consisted of noninjured rats. No treatment was given in the ischemia-reperfusion group; whereas 1 ml saline and 600 mg/kg magnesium sulfate was administered in the vehicle and the treatment groups 30 min before ischemia reperfusion injury. Lipid peroxidation in the brain tissue was determined as the concentration of thiobarbituric acid-reactive substances (TBARS) for each fetal rat. A one-way analysis of variance and post hoc test were used for statistical analysis.
  
  
  RESULTS
  TBARS levels were found to be increased after ischemia reperfusion injury when compared with controls. Magnesium sulfate treatment prevented the increase in TBARS after ischemia reperfusion injury.
  
  
  CONCLUSIONS
  We have shown that magnesium sulfate decreases TBARS levels significantly in fetal rat brain subjected to ischemia reperfusion injury and may have potential therapeutic benefits by reducing oxidative stress after intrauterine ischemia-reperfusion-induced fetal brain damage.
authors.affiliation: Etlik Zubeyde Hanim Women's Health Teaching & Research Hospital, Ankara, Turkey.
authors.forename: Ayse
authors.initials: A
authors.lastname: Solaroglu
authors.forename: Faruk
authors.initials: F
authors.lastname: Suat Dede
authors.forename: Orhan
authors.initials: O
authors.lastname: Gelisen
authors.forename: Ozlem
authors.initials: O
authors.lastname: Secilmis
authors.forename: Hulya
authors.initials: H
authors.lastname: Dede
mesh_terms: D000818:Animals; D000927:Anticonvulsants / Q000627:therapeutic use*; D001930:Brain Injuries / Q000209:etiology / Q000517:prevention & control*; D002545:Brain Ischemia / Q000150:complications; D004353:Drug Evaluation, Preclinical; D005260:Female; D005315:Fetal Diseases / Q000517:prevention & control*; D008278:Magnesium Sulfate / Q000627:therapeutic use*; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000517:prevention & control*
chemical_list: D000927:Anticonvulsants; D008278:Magnesium Sulfate