title: In vitro concurrent endothelial and osteogenic commitment of adipose-derived stem cells and their genomical analyses through comparative genomic hybridization array: novel strategies to increase the successful engraftment of tissue-engineered bone grafts.
abstract: In the field of tissue engineering, adult stem cells are increasingly recognized as an important tool for in vitro reconstructed tissue-engineered grafts. In the world of cell therapies, undoubtedly, mesenchymal stem cells from bone marrow or adipose tissue are the most promising progenitors for tissue engineering applications. In this setting, adipose-derived stem cells (ASCs) are generally similar to those derived from bone marrow and are most conveniently extracted from tissue removed by elective cosmetic liposuction procedures; they also show a great potential for endothelization. The aim of the present work was to investigate how the cocommitment into a vascular and bone phenotype of ASCs could be a useful tool for improving the in vitro and in vivo reconstruction of a vascularized bone graft. Human ASCs obtained from abdominoplasty procedures were loaded in a hydroxyapatite clinical-grade scaffold, codifferentiated, and tested for proliferation, cell distribution, and osteogenic and vasculogenic gene expression. The chromosomal stability of the cultures was investigated using the comparative genomic hybridization array for 3D cultures. ASC adhesion, distribution, proliferation, and gene expression not only demonstrated a full osteogenic and vasculogenic commitment in vitro and in vivo, but also showed that endothelization strongly improves their osteogenic commitment. In the end, genetic analyses confirmed that no genomical alteration in long-term in vitro culture of ASCs in 3D scaffolds occurs.
authors.affiliation: Department of Histology, Microbiology, and Medical Biotechnology, University of Padova, Padova, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Gardin
authors.forename: Eriberto
authors.initials: E
authors.lastname: Bressan
authors.forename: Letizia
authors.initials: L
authors.lastname: Ferroni
authors.forename: Elisa
authors.initials: E
authors.lastname: Nalesso
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Vindigni
authors.forename: Edoardo
authors.initials: E
authors.lastname: Stellini
authors.forename: Paolo
authors.initials: P
authors.lastname: Pinton
authors.forename: Stefano
authors.initials: S
authors.lastname: Sivolella
authors.forename: Barbara
authors.initials: B
authors.lastname: Zavan
mesh_terms: D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D000328:Adult; D000818:Animals; D016025:Bone Transplantation / Q000379:methods; D002454:Cell Differentiation / Q000235:genetics; D049109:Cell Proliferation; D002478:Cells, Cultured; D055028:Comparative Genomic Hybridization; D017886:Durapatite / Q000737:chemistry; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D010012:Osteogenesis / Q000235:genetics; D051381:Rats; D011923:Rats, Nude; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry; D014183:Transplantation, Heterologous; D055815:Young Adult
chemical_list: D017886:Durapatite