title: [Micro- and ultrastructural characteristics of liver and nuclear endonuclease activity in hepatocytes after the combined exposure to industrial frequency magnetic field and continuous illumination].
abstract: The effects of combined action of the industrial frequency magnetic field (MF) and continuous illumination (CI) on microcirculation, ultrastructural features of liver cells and apoptosis were studied in Wistar rats (n=56). It was found that in the liver of rats exposed to MF, CI and CI+MF for 14 days, parenchyma relative area was reduced, and that occupied by the sinusoidal capillaries was increased together with the liver vascularization coefficient. Combined exposure resulted in most pronounced ultrastructural changes (plasma membrane lysis in hepatocytes and endothelial cells, doubling of osmiophilic layers in tight intercellular junctions). Hepatocytes revealed condensed cytoplasm, mitochondria with electron-dense matrix, nuclear chromatin disintegration and nucleolar compaction. Hepatocyte intranuclear endonucleases were activated.
authors.forename: S V
authors.initials: SV
authors.lastname: Michurina
authors.forename: Iu I
authors.initials: IuI
authors.lastname: Borodin
authors.forename: V A
authors.initials: VA
authors.lastname: Trufakin
authors.forename: A D
authors.initials: AD
authors.lastname: Belkin
authors.forename: G M
authors.initials: GM
authors.lastname: Vakulin
authors.forename: P M
authors.initials: PM
authors.lastname: Larionov
authors.forename: A V
authors.initials: AV
authors.lastname: Shurlygina
mesh_terms: D000818:Animals; D017209:Apoptosis; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D002467:Cell Nucleus / Q000201:enzymology / Q000648:ultrastructure; D003593:Cytoplasm / Q000378:metabolism / Q000648:ultrastructure; D004720:Endonucleases / Q000378:metabolism*; D022781:Hepatocytes / Q000201:enzymology / Q000648:ultrastructure; D008029:Lighting / Q000009:adverse effects*; D008099:Liver / Q000201:enzymology / Q000648:ultrastructure; D008280:Magnetics; D008297:Male; D008930:Mitochondria, Liver / Q000378:metabolism / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
chemical_list: D004720:Endonucleases