title: Tissue microarray analysis of topoisomerase IIalpha protein in gastric adenocarcinomas: histogenetic and prognostic implications.
abstract: The aim of this study was to: To investigate topoisomerase IIα (topo-IIα) expression and its correlation with clinicopathological parameters in primary gastric cancer patients.
  
  
  METHODS
  A tissue microarray including tumor, paired non-tumoral and lymph node metastasis specimens from 210 gastric adenocarcinoma patients was built for immunohistochemical interrogation. The correlation between topo-IIα expression and patient clinicopathological parameters was evaluated by univariate and multivariate analyses.
  
  
  RESULTS
  High topo-IIα expression was observed in 30.00% (63/210) of the primary tumors, 25.27% (23/91) of the lymph node metastases and 0.47% (1/210) of the non-tumoral gastric mucosa (p<0.001). Topo-IIα expression in the gastric adenocarcinoma was positively correlated with tumor location (gastric cardia, p<0.001), intestinal histological type (p=0.041), late age onset of gastric adenocarcinoma (≥50 years; p=0.002) and male gender (p=0.038). There was no association with other clinicopathological parameters. No correlation was observed between topo-IIα expression and survival.
  
  
  CONCLUSIONS
  The prognostic value of topo-IIα in gastric adenocarcinoma remains underdetermined.
authors.affiliation: Department of Geriatric Gastroenterology, China PLA General Hospital, Beijing, PR China. wanggangshi@hotmail.com
authors.forename: Gangshi
authors.initials: G
authors.lastname: Wang
authors.forename: Haili
authors.initials: H
authors.lastname: Huang
authors.forename: Jie
authors.initials: J
authors.lastname: Gao
authors.forename: Ping
authors.initials: P
authors.lastname: Chen
authors.forename: Weidi
authors.initials: W
authors.lastname: You
authors.forename: Benyan
authors.initials: B
authors.lastname: Wu
authors.forename: Mengwei
authors.initials: M
authors.lastname: Wang
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology* / Q000473:pathology; D000951:Antigens, Neoplasm / Q000378:metabolism*; D004250:DNA Topoisomerases, Type II / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005260:Female; D006801:Humans; D008019:Life Style; D008297:Male; D008875:Middle Aged; D013274:Stomach Neoplasms / Q000201:enzymology* / Q000473:pathology; D046888:Tissue Array Analysis / Q000379:methods*
chemical_list: D000951:Antigens, Neoplasm; D004268:DNA-Binding Proteins; D004250:DNA Topoisomerases, Type II