title: Differences in the priming effect of various clades/subclades of inactivated H5N1 vaccine for booster injection with heterologous clades of vaccine strains.
abstract: The prime-boost response induced by different combinations of four H5N1 vaccines (NIBRG-14 (clade 1), Indo05/2005(H5N1)/PR8-IBCDC-RG2 (clade 2.1), A/Bar-Headed Goose/Qinhai Lake/1A/05 SJ163222 (clade 2.2), and Anhui01/2005(H5N1)-PR8-IBCDC-RG5 (clade 2.3.4)) was evaluated in mice. Clade 1-primed BALB/c mice showed a booster response to all of the other three H5N1 vaccines. Clade 2.2 vaccine was also a good priming vaccine. However, mice primed with clade 2.1 or clade 2.3.4 vaccine did not respond to booster injection with clade 1 vaccine, suggesting that priming might actually inhibit the booster response with some combinations of vaccines belonging to different clades. Analysis of the mechanism involved showed that lymphocytes from primed mice secreted comparable amounts of cytokines with any combination of priming and booster vaccines. Therefore, impairment of B cell immunity specific to certain booster strains may have been involved.
authors.affiliation: The Chemo-Sero-Therapeutic Research Institute, Kikuchi Research Center, Kawabe Kyokushi, Kikuchi, Kumamoto 869-1298, Japan. ikeno-da@kaketsuken.or.jp
authors.forename: Daisuke
authors.initials: D
authors.lastname: Ikeno
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Kimachi
authors.forename: Kayo
authors.initials: K
authors.lastname: Ibaragi
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kudo
authors.forename: Shuro
authors.initials: S
authors.lastname: Goto
authors.forename: Koichi
authors.initials: K
authors.lastname: Odoh
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Itamura
authors.forename: Takato
authors.initials: T
authors.lastname: Odagiri
authors.forename: Masato
authors.initials: M
authors.lastname: Tashiro
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Kino
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D016207:Cytokines / Q000378:metabolism; D005260:Female; D005838:Genotype; D006385:Hemagglutination Inhibition Tests; D007117:Immunization, Secondary / Q000379:methods*; D053124:Influenza A Virus, H5N1 Subtype / Q000145:classification / Q000235:genetics* / Q000276:immunology*; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D014611:Vaccination / Q000379:methods*; D015164:Vaccines, Inactivated / Q000008:administration & dosage / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D016207:Cytokines; D007252:Influenza Vaccines; D015164:Vaccines, Inactivated