title: Protein engineering of α/β-hydrolase fold enzymes.
abstract: The superfamily of α/β-hydrolase fold enzymes is one of the largest known protein families, including a broad range of synthetically useful enzymes such as lipases, esterases, amidases, hydroxynitrile lyases, epoxide hydrolases and dehalogenases. This minireview covers methods developed for efficient protein engineering of these enzymes. Special emphasis is placed on the alteration of enzyme properties such as substrate range, thermostability and enantioselectivity for their application in biocatalysis. In addition, concepts for the investigation of the evolutionary relationship between the different members of this protein superfamily are covered, together with successful examples.
authors.affiliation: Department of Biotechnology and Enzyme Catalysis, Institute of Biochemistry, Greifswald University, Greifswald, Germany.
authors.forename: Helge
authors.initials: H
authors.lastname: Jochens
authors.forename: Martin
authors.initials: M
authors.lastname: Hesseler
authors.forename: Konstanze
authors.initials: K
authors.lastname: Stiba
authors.forename: Santosh Kumar
authors.initials: SK
authors.lastname: Padhi
authors.forename: Romas J
authors.initials: RJ
authors.lastname: Kazlauskas
authors.forename: Uwe T
authors.initials: UT
authors.lastname: Bornscheuer
mesh_terms: D000595:Amino Acid Sequence; D001419:Bacteria / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D019020:Directed Molecular Evolution / Q000379:methods*; D005658:Fungi / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D006867:Hydrolases / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D015202:Protein Engineering / Q000379:methods*; D016415:Sequence Alignment
chemical_list: D006867:Hydrolases