title: Low dose of fine particulate matter (PM2.5) can induce acute oxidative stress, inflammation and pulmonary impairment in healthy mice.
abstract: Air pollution is associated with morbidity and mortality induced by respiratory diseases. However, the mechanisms therein involved are not yet fully clarified. Thus, we tested the hypothesis that a single acute exposure to low doses of fine particulate matter (PM2.5) may induce functional and histological lung changes and unchain inflammatory and oxidative stress processes. PM2.5 was collected from the urban area of São Paulo city during 24 h and underwent analysis for elements and polycyclic aromatic hydrocarbon contents. Forty-six male BALB/c mice received intranasal instillation of 30 μL of saline (CTRL) or PM2.5 at 5 or 15 μg in 30 μL of saline (P5 and P15, respectively). Twenty-four hours later, lung mechanics were determined. Lungs were then prepared for histological and biochemical analysis. P15 group showed significantly increased lung impedance and alveolar collapse, as well as lung tissue inflammation, oxidative stress and damage. P5 presented values between CTRL and P15: higher mechanical impedance and inflammation than CTRL, but lower inflammation and oxidative stress than P15. In conclusion, acute exposure to low doses of fine PM induced lung inflammation, oxidative stress and worsened lung impedance and histology in a dose-dependent pattern in mice.
authors.affiliation: Laboratory of Respiration Physiology, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: D R
authors.initials: DR
authors.lastname: Riva
authors.forename: C B
authors.initials: CB
authors.lastname: Magalhães
authors.forename: A A
authors.initials: AA
authors.lastname: Lopes
authors.forename: T
authors.initials: T
authors.lastname: Lanças
authors.forename: T
authors.initials: T
authors.lastname: Mauad
authors.forename: O
authors.initials: O
authors.lastname: Malm
authors.forename: S S
authors.initials: SS
authors.lastname: Valença
authors.forename: P H
authors.initials: PH
authors.lastname: Saldiva
authors.forename: D S
authors.initials: DS
authors.lastname: Faffe
authors.forename: W A
authors.initials: WA
authors.lastname: Zin
mesh_terms: D000818:Animals; D002947:Cities; D004305:Dose-Response Relationship, Drug; D019803:Glutathione Disulfide / Q000378:metabolism; D008168:Lung / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D055370:Lung Injury / Q000139:chemically induced* / Q000378:metabolism / Q000503:physiopathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018384:Oxidative Stress / Q000187:drug effects*; D010088:Oxidoreductases / Q000378:metabolism; D010316:Particle Size; D052638:Particulate Matter / Q000737:chemistry / Q000633:toxicity*; D012129:Respiratory Function Tests
chemical_list: D052638:Particulate Matter; D010088:Oxidoreductases; D019803:Glutathione Disulfide