title: Identification and quantification of a major anti-oxidant and anti-inflammatory phenolic compound found in basil, lemon thyme, mint, oregano, rosemary, sage, and thyme.
abstract: Major phenolic compounds from basil, lemon thyme, mint, oregano, rosemary, sage, and thyme were investigated using a high-performance liquid chromatography (HPLC) profiling technique in combination with DPPH-radical scavenging, xanthine oxidase and cyclooxygenase assays. For the present study, 15 plant-derived phenolic compounds (gallic, protocatechuic, vanillic, syringic, chlorogenic, rosmarinic, caffeic, ferulic, and sinapic acids, protocatechualdehyde, vanillin, N-coumaroyltyramine, N-caffeoyltyramine, N-feruloyltyramine, and N-sinapoyltyramine) were selected and their DPPH-radical scavenging activities were first determined. Then, a standard HPLC profiling of these phenolics was constructed using an HPLC method to isolate anti-oxidant and anti-inflammatory phenolic compounds from MeOH extracts of the plants. Rosmarinic acid was identified as a major anti-oxidant compound (0.22-0.97%) in all seven herbs, confirmed by nuclear magnetic resonance. Rosmarinic acid from the plants quenched superoxide radicals from xanthine oxidase and inhibited cyclooxygenase I and II enzymes. In this study, the rosmarinic acid content of perilla was also determined and compared with those of the seven herbs.
authors.affiliation: Diet, Genomics, and Immunology Laboratory, US Department of Agriculture, Beltsville, Maryland 20705, USA. jae.park@ars.usda.gov
authors.forename: Jae B
authors.initials: JB
authors.lastname: Park
mesh_terms: D000975:Antioxidants / Q000032:analysis*; D001713:Biphenyl Compounds / Q000378:metabolism; D016861:Cyclooxygenase Inhibitors / Q000032:analysis*; D019686:Lamiaceae / Q000737:chemistry*; D010636:Phenols / Q000032:analysis* / Q000494:pharmacology*; D010851:Picrates / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D013481:Superoxides / Q000378:metabolism; D014969:Xanthine Oxidase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D016861:Cyclooxygenase Inhibitors; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D013481:Superoxides; C004931:1,1-diphenyl-2-picrylhydrazyl; D014969:Xanthine Oxidase