title: Arabidopsis monothiol glutaredoxin, AtGRXS17, is critical for temperature-dependent postembryonic growth and development via modulating auxin response.
abstract: Global environmental temperature changes threaten innumerable plant species. Although various signaling networks regulate plant responses to temperature fluctuations, the mechanisms unifying these diverse processes are largely unknown. Here, we demonstrate that an Arabidopsis monothiol glutaredoxin, AtGRXS17 (At4g04950), plays a critical role in redox homeostasis and hormone perception to mediate temperature-dependent postembryonic growth. AtGRXS17 expression was induced by elevated temperatures. Lines altered in AtGRXS17 expression were hypersensitive to elevated temperatures and phenocopied mutants altered in the perception of the phytohormone auxin. We show that auxin sensitivity and polar auxin transport were perturbed in these mutants, whereas auxin biosynthesis was not altered. In addition, atgrxs17 plants displayed phenotypes consistent with defects in proliferation and/or cell cycle control while accumulating higher levels of reactive oxygen species and cellular membrane damage under high temperature. Together, our findings provide a nexus between reactive oxygen species homeostasis, auxin signaling, and temperature responses.
authors.affiliation: United States Department of Agriculture/Agricultural Research Service, Children's Nutrition Research Center, Baylor College of Medicine, Houston, Texas 77030, USA. ncheng@bcm.tmc.edu
authors.forename: Ning-Hui
authors.initials: NH
authors.lastname: Cheng
authors.forename: Jian-Zhong
authors.initials: JZ
authors.lastname: Liu
authors.forename: Xing
authors.initials: X
authors.lastname: Liu
authors.forename: Qingyu
authors.initials: Q
authors.lastname: Wu
authors.forename: Sean M
authors.initials: SM
authors.lastname: Thompson
authors.forename: Julie
authors.initials: J
authors.lastname: Lin
authors.forename: Joyce
authors.initials: J
authors.lastname: Chang
authors.forename: Steven A
authors.initials: SA
authors.lastname: Whitham
authors.forename: Sunghun
authors.initials: S
authors.lastname: Park
authors.forename: Jerry D
authors.initials: JD
authors.lastname: Cohen
authors.forename: Kendal D
authors.initials: KD
authors.lastname: Hirschi
mesh_terms: D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development*; D029681:Arabidopsis Proteins / Q000096:biosynthesis* / Q000235:genetics; D001692:Biological Transport / Q000502:physiology; D002453:Cell Cycle / Q000502:physiology; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D054477:Glutaredoxins / Q000096:biosynthesis* / Q000235:genetics; D006706:Homeostasis / Q000502:physiology; D006358:Hot Temperature; D007210:Indoleacetic Acids / Q000378:metabolism*; D009154:Mutation; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology
chemical_list: D029681:Arabidopsis Proteins; C578673:AtGRXS17 protein, Arabidopsis; D054477:Glutaredoxins; D007210:Indoleacetic Acids; D017382:Reactive Oxygen Species