title: Negative effects of alcohol intake and estrogen deficiency combination on osseointegration in a rat model.
abstract: Alcohol intake and estrogen deficiency can both affect bone physiology and have shown to have an adverse effect on dental implant therapy. However, the combination of both factors on osseointegration is unknown. The aim of this study was to evaluate osseointegration in rats fed with alcohol and presenting induced estrogen deficiency. Ninety-six female rats were divided according to diet and hormonal condition into 6 groups as follows: group Sh-W: sham (simulated ovariectomy) control, food and water ad libitum; group Sh-Et: sham, food and 20% ethanol solution ad libitum; group Sh-Su: sham, food and sucrose solution controlled to ensure an isocaloric diet in relation to Sh-Et; group Ov-W: ovariectomy, food and water ad libitum; group Ov-Et: ovariectomy, food and 20% ethanol solution ad libitum; and group Ov-Su: ovariectomy, food and sucrose solution controlled to ensure an isocaloric diet as Ov-Et. The groups were subdivided according to time of euthanasia: 30 and 45 days after placement of implants. Implant surgery was performed 1 month after ovariectomy or sham. After euthanasia, the femurs were removed and evaluated by histomorphometry. Groups Ov-Et and Ov-Su showed the lowest percentage of bone-to-implant contact. The combination of alcohol intake and estrogen deficiency, and the combination of estrogen deficiency and reduced ingestion of food can negatively affect osseointegration in rats.
authors.affiliation: Department of Biosciences and Oral Diagnosis, São José dos Campos School of Dentistry, São Paulo State University, São Paulo, Brazil.
authors.forename: Camila Porto
authors.initials: CP
authors.lastname: de Deco
authors.forename: Adriana Mathias Pereira
authors.initials: AM
authors.lastname: da Silva Marchini
authors.forename: Mary Anne Moreira
authors.initials: MA
authors.lastname: Bárbara
authors.forename: Luana Marotta Reis
authors.initials: LM
authors.lastname: de Vasconcellos
authors.forename: Rosilene Fernandes
authors.initials: RF
authors.lastname: da Rocha
authors.forename: Leonardo
authors.initials: L
authors.lastname: Marchini
mesh_terms: D000428:Alcohol Drinking / Q000009:adverse effects; D000818:Animals; D015921:Dental Implants; D004967:Estrogens / Q000172:deficiency*; D000431:Ethanol / Q000009:adverse effects*; D005260:Female; D005269:Femur / Q000601:surgery; D020007:Implants, Experimental; D016348:Osseointegration; D010052:Ovariectomy; D051381:Rats; D017208:Rats, Wistar
chemical_list: D015921:Dental Implants; D004967:Estrogens; D000431:Ethanol