title: Mitochondrial and energy metabolism-related properties as novel indicators of lung cancer stem cells.
abstract: Energy metabolism is the foundation of survival for all organisms, and mitochondria are the most important energy-supplying organelles in eukaryotic cells. However, the mitochondrial and energy/metabolism-related properties of cancer stem cells (CSCs), the stem cell-like subpopulation in tumor masses, remain unknown. In our study, we compared the masses of mitochondria and mitochondrial DNA (mtDNA), the mitochondrial membrane potential (Δψm), oxygen/glucose consumption, and the concentration of reactive oxygen species (ROS) and ATP between lung CSCs (LCSCs) and non-LCSCs. In addition, the change in features during differentiation was examined. Some mitochondrial and energy metabolism-related properties, such as perinuclear mitochondrial distribution, a lower quantity of mtDNA, higher Δψm, lower oxygen/glucose consumption, and lower intracellular concentrations of ROS and ATP, can be used as indicators of LCSCs.
authors.affiliation: Institute of Respiratory Disease, Xinqiao Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Xiao-Qun
authors.initials: XQ
authors.lastname: Ye
authors.forename: Qi
authors.initials: Q
authors.lastname: Li
authors.forename: Guang-Hui
authors.initials: GH
authors.lastname: Wang
authors.forename: Fen-Fen
authors.initials: FF
authors.lastname: Sun
authors.forename: Gui-Jun
authors.initials: GJ
authors.lastname: Huang
authors.forename: Xiu-Wu
authors.initials: XW
authors.lastname: Bian
authors.forename: Shi-Cang
authors.initials: SC
authors.lastname: Yu
authors.forename: Gui-Sheng
authors.initials: GS
authors.lastname: Qian
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D017209:Apoptosis; D002454:Cell Differentiation; D004272:DNA, Mitochondrial / Q000235:genetics; D004734:Energy Metabolism; D005947:Glucose / Q000378:metabolism; D006801:Humans; D007124:Immunoenzyme Techniques; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D053078:Membrane Potential, Mitochondrial; D008928:Mitochondria / Q000378:metabolism*; D014411:Neoplastic Stem Cells / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D004272:DNA, Mitochondrial; D017382:Reactive Oxygen Species; D000255:Adenosine Triphosphate; D005947:Glucose; D010100:Oxygen