title: Down-regulation of TM4SF is associated with the metastatic potential of gastric carcinoma TM4SF members in gastric carcinoma.
abstract: BACKGROUND
  The aim of this study was to clarify the clinical significance of TM4SF members CD9, CD63 and CD82 in human gastric carcinoma.
  
  
  METHODS
  By employing RT-PCR and immunohistochemistry, we studied the expression of CD9, CD63 and CD82 in 49 paired tissue specimens of normal gastric mucosa and carcinoma. All tissues were obtained from patients who underwent curative surgery.
  
  
  RESULTS
  All normal gastric epithelium and gastric ulcer tissues strongly expressed transcripts and proteins of CD9, CD63 and CD82 as compared with corresponding controls. We found a significant correlation between CD63 mRNA level and different pM statuses (P = 0.036). Carcinomas in M0 stage revealed a stronger expression of CD63 than carcinomas in M1 stage. Expression of CD9 protein was found significantly stronger in pN0, pM0 than in advanced pN stages (P = 0.03), pM1 (P = 0.013), respectively. We found the relationship between CD63 expression, gender (p = 0.09) and nodal status (p = 0.028), respectively. Additionally, advanced and metastasized tumor tissues revealed significantly down-regulated CD82 protein expression (p = 0.033 and p = 0, respectively), which correlated with the tumor pTNM stage (p = 0.001).
  
  
  CONCLUSIONS
  The reduction of CD9, CD63 and CD82 expression are indicators for the metastatic potential of gastric carcinoma cells. Unlike their expression in other tumor types, the constitutive expression of CD63 may indicate that this factor does play a direct role in human gastric carcinogenesis.
authors.affiliation: Department of General Surgery, The first affiliated Hospital of Wenzhou medical School, Wenzhou 325000, Zhejiang, PR, China. zhouxun.chen@googlemail.com
authors.forename: Zhouxun
authors.initials: Z
authors.lastname: Chen
authors.forename: Suchen
authors.initials: S
authors.lastname: Gu
authors.forename: Bogusz
authors.initials: B
authors.lastname: Trojanowicz
authors.forename: Naxin
authors.initials: N
authors.lastname: Liu
authors.forename: Guanbao
authors.initials: G
authors.lastname: Zhu
authors.forename: Henning
authors.initials: H
authors.lastname: Dralle
authors.forename: Cuong
authors.initials: C
authors.lastname: Hoang-Vu
mesh_terms: D015703:Antigens, CD / Q000235:genetics*; D015536:Down-Regulation; D015870:Gene Expression; D006801:Humans; D051861:Kangai-1 Protein / Q000235:genetics*; D008562:Membrane Glycoproteins / Q000235:genetics*; D010980:Platelet Membrane Glycoproteins / Q000235:genetics*; D011379:Prognosis; D012189:Retrospective Studies; D013274:Stomach Neoplasms / Q000235:genetics*; D060245:Tetraspanin 29; D060149:Tetraspanin 30
chemical_list: D015703:Antigens, CD; C554586:CD63 protein, human; C497688:CD82 protein, human; C554869:CD9 protein, human; D051861:Kangai-1 Protein; D008562:Membrane Glycoproteins; D010980:Platelet Membrane Glycoproteins; D060245:Tetraspanin 29; D060149:Tetraspanin 30