title: [Methylation status of IEX-1 gene promotor CpG island in malignant hematopoietic cell lines].
abstract: This study was to purposed to explore the methylation status changes of IEX-1 gene promoter CpG island and its relevance with occurrence of hematologic malignancies. The methylation status of IEX-1 gene promoter CpG island in 9 NB4, HL-60 U937, Raji, CA46, Jurkat, K562, CEM and Molt4 hematologic malignant cell lines was detected by using methylation-specific PCR, the methylation status of IEX-1 gene promoter CpG island in normal peripheral blood mononuclear cells treated by M. sssI enzyme and the methylation status of IEX-1 gene promoter CpG island in normal peripheral blood mononuclear cells untreated were used as positive and negative controls respectively. The results showed that the hypermethylation of IEX-1 gene promoter CpG islands was detected in NB4, Molt4 and Raji cell lines, as well as in normal peripheral blood mononuclear cells treated by M. sssl enzyme; the partial methylation status was found in CA46, CEM, U937, K562, HL-60 and Jurkat cell lines; the unmethylation status was observed in untreated normal peripheral blood mononuclear cells. It is concluded that the changes of methylation status of gene IEX-1 promoter CpG island correlates with hematologic malignancies to a certain extent.
authors.affiliation: Department of Hematology, Fujian Medical University, Fuzhou 350001, Fujian Province, China.
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Li
authors.forename: Jian-Zhen
authors.initials: JZ
authors.lastname: Shen
authors.forename: Song-Fei
authors.initials: SF
authors.lastname: Shen
authors.forename: Hai-Ying
authors.initials: HY
authors.lastname: Fu
authors.forename: Hua-Rong
authors.initials: HR
authors.lastname: Zhou
authors.forename: Dan-Sen
authors.initials: DS
authors.lastname: Wu
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Zhang
authors.forename: Yong-Qing
authors.initials: YQ
authors.lastname: Zheng
mesh_terms: D051017:Apoptosis Regulatory Proteins / Q000235:genetics*; D045744:Cell Line, Tumor; D018899:CpG Islands; D019175:DNA Methylation; D015972:Gene Expression Regulation, Neoplastic; D019337:Hematologic Neoplasms / Q000235:genetics* / Q000473:pathology; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000473:pathology; D008565:Membrane Proteins / Q000235:genetics*; D011401:Promoter Regions, Genetic
chemical_list: D051017:Apoptosis Regulatory Proteins; C099815:IER3 protein, human; D008565:Membrane Proteins