title: [Myocardial structural changes in acute left ventricular overload in an experiment].
abstract: Acute left ventricular (LV) overload was simulated in rabbits, by applying a metal spiral to narrow the ascending aorta by one third of its baseline diameter. A control group comprised intact animals. Studies were conducted 1, 3, and 5 days after surgery for simulation of LV overload in parallel series. In the first series of the experiment, semifine LV sections were examined by light microscopy and morphometry. The rate of cardiomyocyte (CMC) apoptosis was estimated calculating the free lying nuclei--the morphologically unchanged nuclei that were present in the extracellular space. In the second series, CMC apoptosis underwent immunohistochemical assessment, by running the TUNEL test. During its acute hemodynamic overload, the LV myocardium showed significant tissue destruction and a substantially lower number of viable myofibrils, and an increase in the count of apoptotically altered CMCs.
authors.forename: M L
authors.initials: ML
authors.lastname: Blagonravov
authors.forename: V A
authors.initials: VA
authors.lastname: Koviazin
authors.forename: A Iu
authors.initials: AIu
authors.lastname: Korshunova
authors.forename: I I
authors.initials: II
authors.lastname: Babichenko
authors.forename: V A
authors.initials: VA
authors.lastname: Frolov
mesh_terms: D000818:Animals; D017209:Apoptosis; D004195:Disease Models, Animal; D007150:Immunohistochemistry; D008297:Male; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D032383:Myocytes, Cardiac / Q000378:metabolism / Q000473:pathology; D011817:Rabbits; D018487:Ventricular Dysfunction, Left / Q000378:metabolism / Q000473:pathology