title: Impact of blood processing on estimation of soluble HLA-G.
abstract: HLA-G is a non-classical MHC class I antigen that functions as an immunomodulatory molecule. There are two forms of HLA-G antigens, soluble and membrane bound. Soluble HLA-G can be produced by translation of HLA-G transcripts (HLA-G5, -G6, -G7) and by shedding/proteolytic cleavage of membrane bound antigens (HLA-G1, -G2, -G3, -G4). Soluble as well as membrane bound HLA-G molecules have a direct inhibitory effect on immune responses. The relevance of soluble HLA-G in various pathologic conditions, such as transplantation, autoimmunity, infectious and malignant diseases, has been extensively investigated, however interpretation remains controversial. In this work we analyzed the levels of sHLA-G (sHLA-G1 and HLA-G5) in different blood samples of healthy donors as serum, and blood plasma isolated using anti-coagulant EDTA and heparin, respectively. We found that the levels of sHLA-G (sHLA-G1 and HLA-G5) in blood plasma prepared with EDTA were significantly higher than those observed in plasma with heparin or in serum. Finally we detected the average levels of sHLA-G in females exceeded those of males.
authors.affiliation: Cancer Research Institute, Slovak Academy of Sciences 833 91 Bratislava. exonpola@savba.sk
authors.forename: K
authors.initials: K
authors.lastname: Poláková
authors.forename: E
authors.initials: E
authors.lastname: Bandžuchová
authors.forename: G
authors.initials: G
authors.lastname: Russ
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006680:HLA Antigens / Q000032:analysis* / Q000097:blood*; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I / Q000032:analysis* / Q000097:blood*; D006801:Humans; D008297:Male; D013048:Specimen Handling / Q000379:methods*
chemical_list: D006680:HLA Antigens; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I