title: PICOT is a molecule which binds to anamorsin.
abstract: Anamorsin (AM) (also called CIAPIN-1) is a cell-death-defying factor. AM deficient mice die during late gestation; AM deficient embryos are anemic and very small compared to wild type (WT) embryos. It is thought that AM plays crucial roles in hematopoiesis and embryogenesis. To clarify the mechanisms of AM functions, we performed the yeast-two-hybrid assay to identify AM-interacting molecules; we found that PICOT (PKCθ interacting cousin of thioredoxin) preferentially bound to AM. We also showed that the N-terminal regions of both AM and PICOT were essential for their bindings and the inhibition of interaction of both molecules might lead to the cell growth retardation. Both PICOT and the yeast homolog of AM are known to be iron-sulfur proteins. The phenotype of PICOT deficient mice is very similar to that of anamorsin deficient mice; both mice are embryonic lethal. These data suggest that AM and PICOT might play cooperatively essential roles in embryogenesis as iron-sulfur cluster proteins.
authors.affiliation: Department of Hematology and Oncology, Osaka University Graduate School of Medicine, Japan.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Saito
authors.forename: Hirohiko
authors.initials: H
authors.lastname: Shibayama
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Tanaka
authors.forename: Akira
authors.initials: A
authors.lastname: Tanimura
authors.forename: Itaru
authors.initials: I
authors.lastname: Matsumura
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Kanakura
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D047108:Embryonic Development / Q000235:genetics; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D007506:Iron-Sulfur Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008817:Mice, Mutant Strains; D011490:Protein Disulfide Reductase (Glutathione); D014018:Tissue Distribution; D020798:Two-Hybrid System Techniques
chemical_list: D002352:Carrier Proteins; C557202:Ciapin1 protein, mouse; D047908:Intracellular Signaling Peptides and Proteins; D007506:Iron-Sulfur Proteins; D011490:Protein Disulfide Reductase (Glutathione); C498606:Txnl2 protein, mouse