title: Reciprocal changes of renal neuronal nitric oxide synthase-α and -β associated with renal progression in a neonatal 5/6 nephrectomized rat model.
abstract: BACKGROUND
  Nitric oxide (NO) deficiency contributes to chronic kidney disease progression. NO deficiency could occur for many reasons, one of which is decreased NO synthase (NOS) abundance and/or activity.
  
  
  METHODS
  In these experiments, we studied two groups of male Sprague Dawley rats given sham or surgical excision of both poles of the left kidney (at 2 days of age) followed by sham or surgical removal of the right kidney at 10 days. Rats were sacrificed at 9 weeks of age and the kidneys examined for abundance of neuronal NOS (nNOS)-α and -β, endothelial NOS, arginase II, argininosuccinate synthase and lysate, protein arginine methyltransferase 1, dimethylarginine dimethylamino-hydrolase 1 and 2, as well as renal pathology.
  
  
  RESULTS
  The 5/6 nephrectomy (NX) group showed renal dysfunction, severe rapidly progressing glomerulosclerosis, and hypertension. Renal cortical nNOSα abundance was significantly reduced, whereas nNOSβ abundance was increased in the 5/6 NX group versus sham. Renal endothelial NOS was unchanged. Next, renal protein arginine methyltransferase 1 abundance was higher, whereas dimethylarginine dimethylamino-hydrolase 2 expression was lower in the 5/6 NX group versus sham. Renal arginase II, argininosuccinate synthase, and argininosuccinate lysate abundances were significantly decreased in 5/6 NX rats than those in sham.
  
  
  CONCLUSIONS
  The neonatal kidney is very susceptible to 5/6 NX-induced injury, and, as in adults, reciprocal changes in the nNOSα and nNOSβ in renal cortex occur during progression of chronic kidney disease and may contribute to the injury.
authors.affiliation: Department of Pediatrics, Chang Gung Memorial Hospital-Kaohsiung Medical Center, Chang Gung University, College of Medicine, Taiwan. tainyl@hotmail.com
authors.forename: You-Lin
authors.initials: YL
authors.lastname: Tain
authors.forename: Sid
authors.initials: S
authors.lastname: Ghosh
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Krieg
authors.forename: Chris
authors.initials: C
authors.lastname: Baylis
mesh_terms: D000818:Animals; D007668:Kidney / Q000201:enzymology*; D007674:Kidney Diseases / Q000378:metabolism; D008297:Male; D023421:Models, Animal; D009392:Nephrectomy; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D019001:Nitric Oxide Synthase