title: Prognostic impact of nm23-H1 and PCNA expression in pathologic stage I non-small cell lung cancer.
abstract: BACKGROUND
  The purpose of this study was to evaluate the value of nm23-H1 and proliferating cell nuclear antigen (PCNA) expression as well as other confirmed prognostic factors in predicting the clinical outcome after definitive surgery of pathologic stage I non-small cell lung cancer (NSCLC).
  
  
  METHODS
  Four hundred fifty-two consecutive and non-selected patients who underwent definitive surgery for stage I NSCLC were included in this study. Formalin-fixed paraffin-embedded specimens were stained for nm23-H1 and PCNA, the correlation between the staining and its clinicopathological parameters, and its prognostic power were analyzed statistically.
  
  
  RESULTS
  Of the 452 patients studied, 320 cases (70.8%) were high expression for nm23-H1. A total of 182 carcinomas (40.3%) were PCNA high expression tumors. PCNA expression correlated with serum CEA level (P < 0.001), and differentiation (P < 0.001). In univariate analysis by log-rank test, serum CEA level, pT stage, differentiation, nm23-H1 expression, and PCNA expression were significant prognostic factors (P = 0.037, 0.021, <0.001, 0.042, and 0.014, respectively). In multivariate analysis, pT stage and nm23-H1 expression maintained its independent prognostic influence on overall survival (P = 0.041 and 0.003, respectively).
  
  
  CONCLUSIONS
  nm23-H1 may be a good biomarker to be applied in clinic to predict the prognosis of patients with completely resected pathologic stage I NSCLC.
authors.affiliation: Department of Respiratory Medicine, Jiangyin People's Hospital, Nantong University, Jiangyin, People's Republic of China.
authors.forename: Chengying
authors.initials: C
authors.lastname: Liu
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Weidong
authors.initials: W
authors.lastname: Mao
authors.forename: Lian
authors.initials: L
authors.lastname: Jiang
authors.forename: Hua
authors.initials: H
authors.lastname: Ni
authors.forename: Mingcong
authors.initials: M
authors.lastname: Mo
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000096:biosynthesis*; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism* / Q000401:mortality / Q000473:pathology / Q000601:surgery; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology / Q000601:surgery; D008297:Male; D008875:Middle Aged; D054778:NM23 Nucleoside Diphosphate Kinases / Q000096:biosynthesis*; D009367:Neoplasm Staging; D011379:Prognosis; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis*
chemical_list: D014408:Biomarkers, Tumor; D054778:NM23 Nucleoside Diphosphate Kinases; D018809:Proliferating Cell Nuclear Antigen; C517938:NME1 protein, human