title: Sequence analysis of topoisomerase gene of pseudocowpox virus isolates from camels (Camelus dromedarius).
abstract: Topoisomerase gene of pseudocowposvirus from Indian dromedarian camel was amplified by PCR using the primers of PCPV from Finnish reindeer and cloned into pGEM-T for sequence analysis. Analysis of amino acid identity revealed that Indian PCPV of camel shared 95.9-96.8 with PCPV of reindeer, 96.2-96.5 with ORFV and 87.5 with BPSV.
authors.affiliation: National Research Centre on Camel, Post Bag No. 7, Jorbeer, Bikaner 334 001, Rajasthan, India. camelnag@yahoo.com
authors.forename: G
authors.initials: G
authors.lastname: Nagarajan
authors.forename: Shelesh Kumar
authors.initials: SK
authors.lastname: Swami
authors.forename: Shyam Singh
authors.initials: SS
authors.lastname: Dahiya
authors.forename: G
authors.initials: G
authors.lastname: Sivakumar
authors.forename: Shirish Dadarao
authors.initials: SD
authors.lastname: Narnaware
authors.forename: F C
authors.initials: FC
authors.lastname: Tuteja
authors.forename: N V
authors.initials: NV
authors.lastname: Patil
mesh_terms: D000818:Animals; D002162:Camelus / Q000821:virology*; D016000:Cluster Analysis; D017931:DNA Primers / Q000235:genetics; D004264:DNA Topoisomerases, Type I / Q000235:genetics*; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D005260:Female; D007194:India; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011213:Poxviridae Infections / Q000821:virology*; D010285:Pseudocowpox Virus / Q000235:genetics* / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D017931:DNA Primers; D004279:DNA, Viral; D014764:Viral Proteins; D004264:DNA Topoisomerases, Type I