title: Identification of strain harboring both aac(6')-Ib and aac(6')-Ib-cr variant simultaneously in Escherichia coli and Klebsiella pneumoniae.
abstract: The aac(6')-Ib gene is the most prevalent gene that encodes aminoglycoside-modifying enzymes and confers resistance to tobramycin, kanamycin, and amikacin. The aac(6')-Ib-cr variant gene can induce resistance against aminoglycoside and fluoroquinolone simultaneously. Two main methods, sequence analysis and the restriction enzyme method, can detect the aac(6')-Ib-cr variant in clinical strains. We collected the 85 strains that were believed to be aac(6')-Ib positive from clinical isolates. Among them, 38 strains were the wild-type; the remaining 47 strains were the aac(6')-Ib-cr variant. Of these 47 strains, 19 simultaneously harbored aac(6')-Ib and aac(6')-Ib-cr. Our study aims to report the characteristics of the 19 strains that simultaneously harbored both genes. This study is the first investigation published in Korea of strains that included both aac(6')-Ib and aac(6')-Ib-cr variant.
authors.affiliation: Department of Molecular Biology, Seoul Medical Science Institute/Seoul Clinical Laboratories, Seoul 152-766, Korea.
authors.forename: Yun-Tae
authors.initials: YT
authors.lastname: Kim
authors.forename: Ji-Hyun
authors.initials: JH
authors.lastname: Jang
authors.forename: Hyun-Chul
authors.initials: HC
authors.lastname: Kim
authors.forename: Hyogyeong
authors.initials: H
authors.lastname: Kim
authors.forename: Kyoung-Ryul
authors.initials: KR
authors.lastname: Lee
authors.forename: Kyung Sun
authors.initials: KS
authors.lastname: Park
authors.forename: Hee-Joo
authors.initials: HJ
authors.lastname: Lee
authors.forename: Young-Jin
authors.initials: YJ
authors.lastname: Kim
mesh_terms: D000123:Acetyltransferases / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics*; D005798:Genes, Bacterial; D007711:Klebsiella pneumoniae / Q000201:enzymology / Q000235:genetics*; D009154:Mutation
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D000123:Acetyltransferases; C056532:aminoglycoside N(6')-acetyltransferase