title: Degradation of N-acylhomoserine lactone quorum sensing signaling molecules by potato root surface-associated Chryseobacterium strains.
abstract: N-Acylhomoserine lactones (AHLs) are used as quorum-sensing signaling molecules by many Gram-negative bacteria. Here, 413 bacterial strains were obtained from the roots of potato plants and screened for AHL-degrading bacteria using Chromobacterium violaceum reporter strains. Sixty one isolates degraded N-hexanoyl-L-homoserine lactone (C6-HSL) within 24 h. Based on their 16S rRNA gene sequences, most of these isolates were assigned to the genus Chryseobacterium and divided into eight groups. Most of the strains degraded N-decanoyl-L-homoserine lactone (C10-HSL) and were active against 3-oxo-substituted AHLs. Groups V and VI were more effective at inactivating AHLs. One group V strain, StRB402, showed putative AHL-lactonase activity. This is the first report of AHL-degrading Chryseobacterium strains.
authors.affiliation: Department of Material and Environmental Chemistry, Graduate School of Engineering, Utsunomiya University, Utsunomiya, Tochigi 321–8585, Japan.
authors.forename: Rumana
authors.initials: R
authors.lastname: Rashid
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Morohoshi
authors.forename: Nobutaka
authors.initials: N
authors.lastname: Someya
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Ikeda
mesh_terms: D054742:Acyl-Butyrolactones / Q000378:metabolism*; D002861:Chromobacterium / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D045247:Chryseobacterium / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D015964:Gene Expression Regulation, Bacterial; D014644:Genetic Variation; D007564:Japan; D010802:Phylogeny; D018517:Plant Roots / Q000378:metabolism; D053038:Quorum Sensing; D012329:RNA, Bacterial / Q000032:analysis / Q000235:genetics; D015398:Signal Transduction; D011198:Solanum tuberosum / Q000382:microbiology*
chemical_list: D054742:Acyl-Butyrolactones; D012329:RNA, Bacterial