title: Measurement of urinary N-telopeptides from type I collagen using a colloidal gold-based immunoassay.
abstract: This study aimed to investigate precision of a newly developed gold nanoparticle-based immunoassay for measuring urinary N-telopeptides (NTx) and its correlation with ELISA measurement. NTx concentrations were determined by absorption changes using spectroscopy at 535 nm, which were measured three times with an interval of two hours for precision test, using urine samples of 60 subjects (32 males and 28 females; mean age 30.4 years, SD 21.9 years). The correlation of the immunoassay with the measurement of ELISA kit was also analyzed. Precision of the immunoassay and its correlation with ELISA were 0.924 (ICC, 95% confidence interval, 0.661-0.971) and 0.90 (p<0.001). Our findings suggested that this immunoassay of urinary NTx could be used as a means of screening for bone resorption and point-of-care testing.
authors.affiliation: Korea Electronics Technology Institute, Yatap-dong, Bundang-gu, Sungnam City, Gyeonggi Do 463-816, South Korea.
authors.forename: Min-Ho
authors.initials: MH
authors.lastname: Lee
authors.forename: Chin Youb
authors.initials: CY
authors.lastname: Chung
authors.forename: Kyoung Min
authors.initials: KM
authors.lastname: Lee
authors.forename: Woo Kyeong
authors.initials: WK
authors.lastname: Seong
authors.forename: Kyung Shik
authors.initials: KS
authors.lastname: Kim
authors.forename: Sang Dae
authors.initials: SD
authors.lastname: Lee
authors.forename: Moon Seok
authors.initials: MS
authors.lastname: Park
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000652:urine; D001862:Bone Resorption / Q000652:urine; D024042:Collagen Type I / Q000378:metabolism* / Q000652:urine; D005260:Female; D017979:Gold Colloid / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008297:Male; D053768:Metal Nanoparticles / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D010455:Peptides / Q000378:metabolism / Q000652:urine*; D011446:Prospective Studies; D018709:Statistics, Nonparametric
chemical_list: D015415:Biomarkers; D024042:Collagen Type I; D017979:Gold Colloid; D010455:Peptides; C052929:collagen type I trimeric cross-linked peptide