title: Quantitative analysis of shape and volume changes in activated thrombocytes in real time by single-shot spatial light modulator-based differential interference contrast imaging.
abstract: We suggest to use a combination of optical tweezers and single-image quantitative differential interference contrast (DIC) emulated by a spatial light modulator (SLM) to study physiological shape changes in thrombocytes after activation and demonstrate the effectiveness of this system for the given task. A specially designed phase mask displayed at the SLM enables quantitative phase calculation from only a single recording. The optical tweezers stabilize trapped thrombocytes for long-time monitoring of changes in the optical thickness profile of thrombocytes during activation by adenosine diphosphate (ADP).
authors.affiliation: Division for Biomedical Physics, Innsbruck Medical University, 6020 Innsbruck, Austria. saranjam.khan@i-med.ac.at
authors.forename: Saranjam
authors.initials: S
authors.lastname: Khan
authors.forename: Alexander
authors.initials: A
authors.lastname: Jesacher
authors.forename: Walter
authors.initials: W
authors.lastname: Nussbaumer
authors.forename: Stefan
authors.initials: S
authors.lastname: Bernet
authors.forename: Monika
authors.initials: M
authors.lastname: Ritsch-Marte
mesh_terms: D000244:Adenosine Diphosphate / Q000737:chemistry; D001792:Blood Platelets / Q000166:cytology*; D003287:Contrast Media; D003952:Diagnostic Imaging / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D008027:Light; D008857:Microscopy, Interference / Q000295:instrumentation / Q000379:methods*; D052898:Optical Tweezers; D015539:Platelet Activation; D013997:Time Factors
chemical_list: D003287:Contrast Media; D000244:Adenosine Diphosphate