title: [Determination of the content of phloridzin using reversed-phase high-performance liquid chromatography].
abstract: OBJECTIVE
  To establish a reversed-phase high-performance liquid chromatographic (RP-HPLC) method for determination of phloridzin content.
  
  
  METHODS
  A RP-HPLC method was established for determination of phloridzin using an Inertsil ODS-3 (4.6×150 mm, 5 µm) column with the detection wavelength of 288 nm, flow rate of 1.0 ml/min, and column temperature of 25 degrees celsius;.
  
  
  RESULTS
  The result showed that the phloridzin had a good linear relationship when its concentration ranged between 0.5988 and 89.72 µg/ml. The regression equation was Y=46.370 X-0.6728 (r=0.9999, n=3). The average recovery of phloridzin was 99.40% with the relative standard deviations (RSD) of 0.67%.
  
  
  CONCLUSIONS
  This method is simple, quick and accurate for determination of phloridzin content.
authors.affiliation: Department of Pharmacy, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
authors.forename: Jian-Rong
authors.initials: JR
authors.lastname: He
authors.forename: Xue-Ying
authors.initials: XY
authors.lastname: Liu
authors.forename: Qing-Wei
authors.initials: QW
authors.lastname: Wang
authors.forename: Zi-Chen
authors.initials: ZC
authors.lastname: Ye
authors.forename: Min
authors.initials: M
authors.lastname: Chang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D010695:Phlorhizin / Q000032:analysis*
chemical_list: D010695:Phlorhizin