title: In vitro targeted magnetic delivery and tracking of superparamagnetic iron oxide particles labeled stem cells for articular cartilage defect repair.
abstract: To assess a novel cell manipulation technique of tissue engineering with respect to its ability to augment superparamagnetic iron oxide particles (SPIO) labeled mesenchymal stem cells (MSCs) density at a localized cartilage defect site in an in vitro phantom by applying magnetic force. Meanwhile, non-invasive imaging techniques were use to track SPIO-labeled MSCs by magnetic resonance imaging (MRI). Human bone marrow MSCs were cultured and labeled with SPIO. Fresh degenerated human osteochondral fragments were obtained during total knee arthroplasty and a cartilage defect was created at the center. Then, the osteochondral fragments were attached to the sidewalls of culture flasks filled with phosphate-buffered saline (PBS) to mimic the human joint cavity. The SPIO-labeled MSCs were injected into the culture flasks in the presence of a 0.57 Tesla (T) magnetic force. Before and 90 min after cell targeting, the specimens underwent T2-weighted turbo spin-echo (SET2WI) sequence of 3.0 T MRI. MRI results were compared with histological findings. Macroscopic observation showed that SPIO-labeled MSCs were steered to the target region of cartilage defect. MRI revealed significant changes in signal intensity (P<0.01). HE staining exibited that a great number of MSCs formed a three-dimensional (3D) cell "sheet" structure at the chondral defect site. It was concluded that 0.57 T magnetic force permits spatial delivery of magnetically labeled MSCs to the target region in vitro. High-field MRI can serve as an very sensitive non-invasive technique for the visualization of SPIO-labeled MSCs.
authors.affiliation: Center of Joint Surgery, Southwest Hospital, the Third Military Medical University, Chongqing, 400038, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Department of Orthopedics, Haikou Hospital, Xiangya Medical College, Central South University, Hainan, 570208, China.
authors.forename: Xuhong
authors.initials: X
authors.lastname: Jin
authors.affiliation: Center of Joint Surgery, Southwest Hospital, the Third Military Medical University, Chongqing, 400038, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Dai
authors.affiliation: Center of Joint Surgery, Southwest Hospital, the Third Military Medical University, Chongqing, 400038, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.affiliation: Center of Joint Surgery, Southwest Hospital, the Third Military Medical University, Chongqing, 400038, China.
authors.forename: Jiarong
authors.initials: J
authors.lastname: Chen
authors.affiliation: Center of Joint Surgery, Southwest Hospital, the Third Military Medical University, Chongqing, 400038, China. jointsurgery@163.com.
authors.forename: Liu
authors.initials: L
authors.lastname: Yang
mesh_terms: D001854:Bone Marrow Cells / Q000166:cytology; D001861:Bone Regeneration; D002358:Cartilage, Articular / Q000293:injuries* / Q000502:physiology; D002478:Cells, Cultured; D005290:Ferric Compounds; D052203:Ferrosoferric Oxide; D006801:Humans; D007270:Injections, Intra-Articular; D008279:Magnetic Resonance Imaging; D008280:Magnetics; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D053758:Nanoparticles
chemical_list: D005290:Ferric Compounds; C000499:ferric oxide; D052203:Ferrosoferric Oxide