title: Admixture mapping to identify spontaneous preterm birth susceptibility loci in African Americans.
abstract: OBJECTIVE
  Preterm birth is 1.5 times more common in African American (17.8%) than European American women (11.5%), even after controlling for confounding variables. We hypothesize that genetic factors may account for this disparity and can be identified by admixture mapping.
  
  
  METHODS
  This is a secondary analysis of women with at least one prior spontaneous preterm birth enrolled in a multicenter prospective study. DNA was extracted and whole-genome amplified from stored saliva samples. Self-identified African American patients were genotyped with a 1,509 single nucleotide polymorphism (SNP) commercially available admixture panel. A logarithm of odds locus-genome score of 1.5 or higher was considered suggestive and 2 or higher was considered significant for a disease locus.
  
  
  RESULTS
  One hundred seventy-seven African American women with one or more prior spontaneous preterm births were studied. One thousand four hundred fifty SNPs were in Hardy-Weinberg equilibrium and passed quality filters. Individuals had a mean of 78.3% to 87.9% African American ancestry for each SNP. A locus on chromosome 7q21-22 was suggestive of an association with spontaneous preterm birth before 37 weeks of gestation (three SNPs with logarithm of odds scores 1.50-1.99). This signal strengthened when women with at least one preterm birth before 35.0 (eight SNPs with logarithm of odds scores greater than 1.50) and before 32.0 weeks of gestation were considered (15 SNPs with logarithm of odds scores greater than 1.50). No other areas of the genome had logarithm of odds scores higher than 1.5.
  
  
  CONCLUSIONS
  Spontaneous preterm birth in African American women may be genetically mediated by a susceptibility locus on chromosome 7. This region contains multiple potential candidate genes, including collagen type 1-α-2 gene and genes involved with calcium regulation.
authors.affiliation: From the Department of Obstetrics and Gynecology at University of Utah, Salt Lake City, Utah; Wake Forest University Health Sciences, Winston-Salem, North Carolina; University of Tennessee, Memphis, Tennessee; University of Alabama at Birmingham, Birmingham, Alabama; The Ohio State University, Columbus, Ohio; University of Pittsburgh, Pittsburgh, Pennsylvania; University of Miami, Miami, Florida; Drexel University, Philadelphia, Pennsylvania; Case Western Reserve University-MetroHealth Medical Center, Cleveland, Ohio; University of Texas Health Science Center at Houston, Houston, Texas; Northwestern University, Chicago, Illinois; The George Washington University Biostatistics Center, Washington, DC; and the Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, Maryland.
authors.forename: Tracy A
authors.initials: TA
authors.lastname: Manuck
authors.forename: Yinglei
authors.initials: Y
authors.lastname: Lai
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Meis
authors.forename: Baha
authors.initials: B
authors.lastname: Sibai
authors.forename: Catherine Y
authors.initials: CY
authors.lastname: Spong
authors.forename: Dwight J
authors.initials: DJ
authors.lastname: Rouse
authors.forename: Jay D
authors.initials: JD
authors.lastname: Iams
authors.forename: Steve N
authors.initials: SN
authors.lastname: Caritis
authors.forename: Mary J
authors.initials: MJ
authors.lastname: O'Sullivan
authors.forename: Ronald J
authors.initials: RJ
authors.lastname: Wapner
authors.forename: Brian
authors.initials: B
authors.lastname: Mercer
authors.forename: Susan M
authors.initials: SM
authors.lastname: Ramin
authors.forename: Alan M
authors.initials: AM
authors.lastname: Peaceman
mesh_terms: D000328:Adult; D001741:Black or African American; D005260:Female; D005819:Genetic Markers; D060005:Genotyping Techniques; D006801:Humans; D020641:Polymorphism, Single Nucleotide; D011247:Pregnancy; D047928:Premature Birth / Q000208:ethnology / Q000235:genetics*; D011446:Prospective Studies
chemical_list: D005819:Genetic Markers