title: Therapeutic effect of ethanolic extract of Hygrophila spinosa T. Anders on gentamicin-induced nephrotoxicity in rats.
abstract: Therapeutic effect of ethanolic extract of Hygrophila spinosa in gentamicin-induced nephrotoxic model of kidney injury in male Sprague-Dawley rats was studied. Rats were administered with gentamicin at a dose of 80 mg/kg intraperitoneally (ip) to induce nephrotoxicity. Kidney function was assessed by measuring serum creatinine and urea. Kidney superoxide dismutase, lipid peroxidation, catalase and reduced glutathione were also measured in control and treated rats. H. spinosa extract showed free radical scavenging activities at doses of 50 and 250 mg/kg with a predominant activity at 250 mg/kg. The ethanolic extract also caused a reduction in serum creatinine and urea levels. Histopathological studies were conducted to confirm the therapeutic action of the plant extract. The results demonstrated that the ethanolic extract of whole plant of H. spinosa evinced the therapeutic effect and inhibited gentamicin-induced proximal tubular necrosis.
authors.affiliation: Department of Pharmacology and Toxicology, Faculty of Veterinary and Animal Sciences, Mannuthy, Thrissur 680 651, India. bibujohn@rediffmail.com
authors.forename: K J
authors.initials: KJ
authors.lastname: Bibu
authors.forename: A D
authors.initials: AD
authors.lastname: Joy
authors.forename: K A
authors.initials: KA
authors.lastname: Mercey
mesh_terms: D027901:Acanthaceae / Q000737:chemistry*; D000818:Animals; D002374:Catalase / Q000378:metabolism; D003404:Creatinine / Q000097:blood; D000431:Ethanol / Q000737:chemistry*; D005839:Gentamicins; D005978:Glutathione / Q000378:metabolism; D007668:Kidney / Q000187:drug effects / Q000201:enzymology / Q000473:pathology*; D007674:Kidney Diseases / Q000097:blood / Q000188:drug therapy* / Q000201:enzymology / Q000473:pathology; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism; D023382:Toxicity Tests, Acute; D014508:Urea / Q000097:blood
chemical_list: D005839:Gentamicins; D010936:Plant Extracts; D000431:Ethanol; D014508:Urea; D003404:Creatinine; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione