title: [Study of rongban tongmai granule on anti-oxidant stress in atherosclerosis].
abstract: OBJECTIVE
  To observe the therapeutic effects of Rongban Tongmai granule on oxidative stress in atherosclerotic rabbits.
  
  
  METHODS
  The experimental rabbits were randomly divided into control group and hyperlipidemic group. The model of experimental atherosclerosis was prepared by feeding high cholesterol and lipid diet for weeks. After 4 weeks, hyperlipidemic rabbits were randomly divided into five groups, model group, high, medium and low doses of Rongban Tongmai granule and Shujiangzhi group, taking medicine for 8 weeks. The level of NO, ox-LDL, LP(a), SOD and MDA in the serum was measured before experiment, after treatment, treated for 4 weeks and for 8 weeks. After treated for 8 weeks, the level of MDA, GSH and NO, as well as activity of SOD and GSH-Px in the liver of rabbits was determined, meanwhile, the pathologic morphology of aortas was observed by light microscope.
  
  
  RESULTS
  Compared with control group, aortic intima of rabbits in model group had obviously thickened and developed atherosclerotic plaque. The serum level of MDA and LP(a) in model group had increased (P < 0.01) at 12 weeks after feeding high cholesterol and lipid diet, but the activity of SOD and level of NO were decreased (P < 0.01, P < 0.001). At the same time, the level of MDA in the liver had been elevated (P < 0.01), but the activity of SOD, GSH and NO was decreased (P < 0.01, P < 0.001) in 12 weeks. Rongban Tong-mai granule could inhibit atherosclerotic lesion in aorta, decrease the level of MDA and LP(a) (P < 0.05), increase the activity of SOD and NO (P < 0.05) after treated for 8 weeks in serum, moreover, the activity of SOD, GSH and NO content in the liver were increased (P < 0.05), as well as MDA was decreased (P < 0.05).
  
  
  CONCLUSIONS
  Rongban Tongmai granule can prevent atherosclerosis by antioxidative stress and correcting unbalance of redox.
authors.affiliation: Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China.
authors.forename: Chengren
authors.initials: C
authors.lastname: Lin
authors.forename: Jianxun
authors.initials: J
authors.lastname: Ren
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.forename: Xueying
authors.initials: X
authors.lastname: Ma
authors.forename: Jianxun
authors.initials: J
authors.lastname: Liu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage*; D050197:Atherosclerosis / Q000097:blood / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D005978:Glutathione / Q000097:blood; D006801:Humans; D008077:Lipoproteins, LDL / Q000097:blood; D008315:Malondialdehyde / Q000097:blood; D018384:Oxidative Stress / Q000187:drug effects; D058226:Plaque, Atherosclerotic; D011817:Rabbits; D011897:Random Allocation; D013482:Superoxide Dismutase / Q000097:blood
chemical_list: D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D005978:Glutathione