title: Naphthalene-degrading bacteria of the genus Rhodococcus from the Verkhnekamsk salt mining region of Russia.
abstract: Eight moderately halotolerant naphthalene-degrading strains of the genus Rhodococcus isolated from soil samples and slime pit bottom sediment of the Verkhnekamsk salt mining region of Russia were characterized by PCR amplification of repetitive bacterial DNA elements (rep-PCR) and identified by 16S ribosomal RNA gene sequence analysis. The diversity of their dioxygenase (nar-like) genes was investigated as these genes are known to be involved in naphthalene-degradation. The analysis of the nar-like genes identified revealed their heterogeneity in the strains under study and identity to the known sequences of nar-like genes of previously characterized from members of the genus Rhodococcus.
authors.affiliation: Laboratory of Chemical Mutagenesis, Institute of Ecology and Genetics of Microorganisms, The Ural Branch of the Russian Academy of Sciences, Perm, Russia. ludaananyina@mail.ru
authors.forename: Lyudmila N
authors.initials: LN
authors.lastname: Anan'ina
authors.forename: Olga V
authors.initials: OV
authors.lastname: Yastrebova
authors.forename: Vitaly A
authors.initials: VA
authors.lastname: Demakov
authors.forename: Elena G
authors.initials: EG
authors.lastname: Plotnikova
mesh_terms: D001483:Base Sequence; D001673:Biodegradation, Environmental; D017124:Conserved Sequence; D004269:DNA, Bacterial / Q000235:genetics; D049308:Dioxygenases / Q000235:genetics*; D005798:Genes, Bacterial; D018740:Genetic Heterogeneity; D005838:Genotype; D008906:Mining; D009281:Naphthalenes / Q000378:metabolism*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012240:Rhodococcus / Q000145:classification / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D012426:Russia; D012965:Sodium Chloride / Q000378:metabolism; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D009281:Naphthalenes; D012336:RNA, Ribosomal, 16S; C031721:naphthalene; D012965:Sodium Chloride; D049308:Dioxygenases