title: Superabsorbent polysaccharide hydrogels based on pullulan derivate as antibacterial release wound dressing.
abstract: To accomplish ideal wound dressing, hydrogels based on a natural polysaccharide, pullulan were synthesized by chemical cross-linking. The tensile strengths of the hydrogel films (1 mm thick) were determined to range from 0.663 to 1.097 MPa in proportion to cross-linking degrees and water contents. The swelling study of the hydrogels in water showed remarkable water absorption property with swelling ratio up to 4000%, which provided the hydrogel with quick hemostatic ability and prevent the wound bed from accumulation of exudates. The water vapor transmission rate and water retention of the hydrogels were found to be in the range of 2213-3498 g/m²/day and 34.74-45.81% (after 6 days), indicating that the hydrogel can maintain a moist environment over wound bed, which could prevent the dehydration of the wound bed and prevent the scab formation. Biocompatibility test revealed that the hydrogels were not cytotoxic. The hydrogel could load antimicrobial agents and effectively suppress bacterial proliferation to protect the wound from bacterial invasion. These results suggest that the pullulan hydrogels prepared in this study may have high potential as new ideal wound-dressing materials.
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, Sichuan 610064, China.
authors.forename: Huanan
authors.initials: H
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.forename: Xiaona
authors.initials: X
authors.lastname: Hu
authors.forename: Jie
authors.initials: J
authors.lastname: Liang
authors.forename: Yujiang
authors.initials: Y
authors.lastname: Fan
authors.forename: Xingdong
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001458:Bandages; D001672:Biocompatible Materials / Q000494:pharmacology; D002478:Cells, Cultured; D003538:Cystamine / Q000737:chemistry; D004926:Escherichia coli / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D005839:Gentamicins / Q000494:pharmacology; D005936:Glucans / Q000737:chemistry*; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D008422:Materials Testing; D051379:Mice; D008826:Microbial Sensitivity Tests; D011134:Polysaccharides / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013211:Staphylococcus aureus / Q000187:drug effects; D013227:Steam; D013718:Tensile Strength / Q000187:drug effects; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D005839:Gentamicins; D005936:Glucans; D011134:Polysaccharides; D013227:Steam; C410955:carboxymethylpullulan; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D003538:Cystamine