title: Role of axonal guidance factor netrin-1 in human placental vascular growth.
abstract: This study investigated the role of netrin-1 in placental vascular development. In vitro rat aortic ring assay and in vivo Matrigel plug assay were conducted to exmaine the effect of netrin-1 on angiogenesis. Human placental microvascular endothelial cells (HPMECs) were isolated and cultured and their viability, migration and tubular formation were studied, in order to examine the effects of netrin-1. The results showed that netrin-1 potently stimulated neovascularization in a mouse Matrigel plug in vivo and the sprouting of endothelial cells in rat aortic rings in vitro. In addition, netrin-1 enhanced the viability, migration and tube formation of HPMECs. Our study suggested that netrin-1 could significantly promote the formation of blood vessels of human placenta and may be a potential target for developing new therapeutic strategies for placental vasculature-related diseases.
authors.affiliation: Department of Gynecology and Obstetrics, Union Hospital, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors.forename: Qianhua
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Gynecology and Obstetrics, Union Hospital, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors.forename: Jianwen
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Department of Gynecology and Obstetrics, Union Hospital, Huazhong University of Science and Technology, Wuhan, 430022, China. whzl027@hotmail.com.
authors.forename: Li
authors.initials: L
authors.lastname: Zou
authors.affiliation: Department of Gynecology and Obstetrics, Union Hospital, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Yang
mesh_terms: D000818:Animals; D001011:Aorta / Q000166:cytology; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D055806:Microvessels / Q000166:cytology*; D018919:Neovascularization, Physiologic / Q000502:physiology*; D009414:Nerve Growth Factors / Q000502:physiology*; D000075388:Netrin-1; D010920:Placenta / Q000098:blood supply*; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D025521:Tumor Suppressor Proteins / Q000502:physiology*
chemical_list: C000617351:NTN1 protein, human; D009414:Nerve Growth Factors; C000617353:Ntn1 protein, mouse; C000617354:Ntn1 protein, rat; D025521:Tumor Suppressor Proteins; D000075388:Netrin-1