title: Characterization of the immune response of human cord-blood derived gamma/delta T cells to stimulation with aminobisphosphonate compounds.
abstract: Vγ9Vδ2 T lymphocytes have been shown to respond to a variety of non-peptide antigens including alkylamines and phosphoantigens. Recently, aminobisphosphonates have also been shown to stimulate this subset of γδ+ T cells. In this study we analyzed the proliferative responses of freshly isolated γδ T lymphocytes obtained from human cord blood when challenged with pyrophosphomonoesters or aminobisphosphonates. Nitrogen-containing aminobisphopsphonates, in contrast to phoshoantigens, readily stimulated expansion of Vδ2Vγ9 cells in human cord blood. Expanded cells displayed an activated mature phenotype, and were capable of producing TNFalpha and IFNgamma but not perforin following secondary stimulation, consistent with the development of a regulatory, as opposed to cytotoxic, phenotype. This approach may provide a useful strategy for a new approach to the treatment of neonatal pathologies.
authors.affiliation: European Center for Brain Research - Santa Lucia Foundation, Rome, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Placido
authors.forename: G
authors.initials: G
authors.lastname: Auricchio
authors.forename: I
authors.initials: I
authors.lastname: Gabriele
authors.forename: E
authors.initials: E
authors.lastname: Galli
authors.forename: E
authors.initials: E
authors.lastname: Brunetti
authors.forename: V
authors.initials: V
authors.lastname: Colizzi
authors.forename: L
authors.initials: L
authors.lastname: Battistini
authors.forename: G
authors.initials: G
authors.lastname: Mancino
mesh_terms: D015703:Antigens, CD / Q000032:analysis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D002478:Cells, Cultured; D004164:Diphosphonates / Q000494:pharmacology*; D005312:Fetal Blood / Q000166:cytology*; D006801:Humans; D016130:Immunophenotyping; D053645:Interleukin-2 Receptor alpha Subunit / Q000032:analysis; D037181:Lectins, C-Type / Q000032:analysis; D008213:Lymphocyte Activation / Q000187:drug effects*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis*; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D000099192:CD69 Antigens
chemical_list: D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D004164:Diphosphonates; D053645:Interleukin-2 Receptor alpha Subunit; D037181:Lectins, C-Type; D016692:Receptors, Antigen, T-Cell, gamma-delta; D000099192:CD69 Antigens; C508017:IL2RA protein, human