title: A coupling process for improving purity of bacterial minicells by holin/lysin.
abstract: Drug and gene delivery systems using bacterial minicells have attracted much attention. Here we attempted to enhance the yield and purity of the minicells using a novel method in which autolysin induced in actively metabolizing parent cells led to autolysis. A two-step protocol coupling an autolysin (holin/lysin) step with a conventional centrifugation step achieved a purity of the minicells similar to that resulting from successive six- or seven-step methods.
authors.affiliation: Department of Biological Sciences, College of Natural Sciences, Chonnam National University, Yongbong-Dong, Buk-Gu, Gwangju 500-757, Korea.
authors.forename: So-Youn
authors.initials: SY
authors.lastname: Park
authors.forename: Jin-Young
authors.initials: JY
authors.lastname: Lee
authors.forename: Woo-Suk
authors.initials: WS
authors.lastname: Chang
authors.forename: Hyon E
authors.initials: HE
authors.lastname: Choy
authors.forename: Geun-Joong
authors.initials: GJ
authors.lastname: Kim
mesh_terms: D001433:Bacteriolysis; D001435:Bacteriophages / Q000235:genetics*; D002499:Centrifugation, Density Gradient / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D015870:Gene Expression; D012475:Salmonella / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D014764:Viral Proteins