title: [Expression of vigilin in cell lines and human hepatocellular carcinoma].
abstract: OBJECTIVE
  To explore the expression status of Vigilin (high density lipoprotein binding protein) in various cell lines and human hepatocellular carcinoma (HCC) tissues.
  
  
  METHODS
  The expression of Vigilin was measured semiquantitatively with western blot in hepatic cancer, cervical cancer and normal cell lines. The samples of 59 hepatocellular carcinoma tissues, 59 adjacent liver tissues and 33 distant non-tumor liver tissues were collected, Vigilin expression in the above samples was detected by immunohistochemistry.
  
  
  RESULTS
  Vigilin expressed in all cell lines, but no expression was found in peripheral blood lymphocytes. The expression levels of Vigilin in tumor cell lines were higher than those in normal cell lines (P < 0.05). Most of the hepatic cells expressed Vigilin, but the expression levels were different (tumor tissues: 0.2226 +/- 0.054, adjacent tissues: 0.2060 +/- 0.056, distant tissues: 0.1820 +/- 0.038, P < 0.001). Highly expression of Vigilin was observed in 54% of tumor tissues, 35% adjacement tissues, and 6% of distant non-tumor tissues, respectively.
  
  
  CONCLUSIONS
  Vigilin may have relationship with HCC progression and proliferation.
authors.affiliation: Department of Biochemistry and Molecular Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Shu-wang
authors.initials: SW
authors.lastname: Duan
authors.forename: Ling
authors.initials: L
authors.lastname: Wei
authors.forename: Wen-li
authors.initials: WL
authors.lastname: Yang
authors.forename: Ke-fei
authors.initials: KF
authors.lastname: Chen
authors.forename: Wen-qing
authors.initials: WQ
authors.lastname: Huang
authors.forename: Yang
authors.initials: Y
authors.lastname: Qin
mesh_terms: D000328:Adult; D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D002460:Cell Line; D045744:Cell Line, Tumor; D005260:Female; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D016601:RNA-Binding Proteins; C075197:high density lipoprotein binding protein