title: [Effect of jinqiaomai on expression of TLR2/4, MyD88 mRNA and IkappaB-alpha in lung tissue of rats with Klebsiella pneumonia].
abstract: OBJECTIVE
  To study the mechanism of protective effect of Jinqiaomai (JQM) on lung injury induced by Klebsiella pneumonia in rats.
  
  
  METHODS
  The model of rats with Klebsiella pneumonia was established. The male SD rats were randomly divided into control group, model group, JQM (6, 3, 1.5 g x kg(-1)) three groups, levofloxacin (25 mg x kg(-1)) group, JQM (3 g x kg(-1)) + levofloxacin (25 mg x kg(-1)) group. The contents of IL-1beta, ICAM-1 and INF-gamma in the lung tissue homogenate were measured by radio-immunoassay and Elisa. TLR2/4 mRNA and MyD88 mRNA expression were detected by RT-PCR. IkappaB-alpha expression was detected by Western Blot.
  
  
  RESULTS
  The rats of model group had obvious lung injury, but those of JQM, JQM + levofloxacin and levofloxacin groups had less injury. The contents of IL-1beta, ICAM-1 and INF-gamma in lung tissue homogenate and the expressions of TLR2/4 mRNA, MyD88 mRNA and IkappaB-alpha in lung of model group were significantly higher than those in the control group (P < 0.05 or P < 0.01), while IL-1beta, ICAM-1 and INF-gamma of JQM groups were significantly lower than those of model group (P < 0.05 or P < 0.01). The expressions of TLR2/4 mRNA, MyD88 mRNA and IkappaB-alpha of JQM (6.3 g x kg(-1)) groups were significantly lower than those of model group(P <0. 05 or P <0. 01).
  
  
  CONCLUSIONS
  The lung injury induced by Klebsiella pneumonia is related to TLR2/4, MyD88 mRNA and IkappaB-alpha. To decrease the excessive expression of TLR2/4, MyD88 mRNA and IkappaB-alpha might be the main mechanism of protective effect of Jinqiaomai on lung injury.
authors.affiliation: Key Laboratory of Antiinflammatory and Immunopharmacology, Ministry of Education, Key Laboratory of Chinese Medicine Research and Development, State Administration of Traditional Chinese Medicine, Department of Pharmacology, Anhui Medical University, Hefei 230032, China. dongly061@yahoo.com.cn
authors.forename: Liuyi
authors.initials: L
authors.lastname: Dong
authors.forename: Chunyan
authors.initials: C
authors.lastname: Wang
authors.forename: Changqing
authors.initials: C
authors.lastname: Wu
authors.forename: Qin
authors.initials: Q
authors.lastname: Jiang
authors.forename: Zhifen
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D020672:I-kappa B Proteins / Q000235:genetics* / Q000378:metabolism; D007710:Klebsiella Infections / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000502:physiology; D008168:Lung / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology*; D008297:Male; D053594:Myeloid Differentiation Factor 88 / Q000235:genetics* / Q000378:metabolism; D000072000:NF-KappaB Inhibitor alpha; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D051195:Toll-Like Receptor 2 / Q000235:genetics* / Q000378:metabolism; D051197:Toll-Like Receptor 4 / Q000235:genetics* / Q000378:metabolism
chemical_list: D004365:Drugs, Chinese Herbal; D020672:I-kappa B Proteins; C507781:Myd88 protein, rat; D053594:Myeloid Differentiation Factor 88; C000606204:NFKBIA protein, human; C000606206:Nfkbia protein, rat; C495343:Tlr2 protein, rat; C495347:Tlr4 protein, rat; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; D000072000:NF-KappaB Inhibitor alpha