title: Grooved surface topography alters matrix-metalloproteinase production by human fibroblasts.
abstract: Extracellular matrix (ECM) remodelling is an essential physiological process in which matrix-metalloproteinases (MMPs) have a key role. Manipulating the manner in which cells produce MMPs and ECMs may enable the creation of a desired tissue type, i.e. effect repair, or the prevention of tissue invasion (e.g. metastasis). The aim of this project was to determine if culturing fibroblasts on grooved topography altered collagen deposition or MMP production. Human fibroblasts were seeded on planar or grooved polycaprolactone substrates (grooves were 12.5 µm wide with varying depths of 240 nm, 540 nm or 2300 nm). Cell behaviour and collagen production were studied using fluorescence microscopy and the spent culture medium was assessed using gel zymography to detect MMPs. Total collagen deposition was high on the 240 nm deep grooves, but decreased as the groove depth increased, i.e. as cell contact guidance decreased. There was an increase in gelatinase on the 2300 nm deep grooved topography and there was a difference in the temporal expression of MMP-3 observed on the planar surface compared to the 540 nm and 2300 nm topographies. These results show that topography can alter collagen and MMP production. A fuller understanding of these processes may permit the design of surfaces tailored to tissue regeneration e.g. tendon repair.
authors.affiliation: Department of Orthopaedics, Southern General Hospital, 1345 Govan Road, Glasgow G51 4TF, UK. alibrydone@gmail.com
authors.forename: Alistair S
authors.initials: AS
authors.lastname: Brydone
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Dalby
authors.forename: Catherine C
authors.initials: CC
authors.lastname: Berry
authors.forename: R M
authors.initials: RM
authors.lastname: Dominic Meek
authors.forename: Laura E
authors.initials: LE
authors.lastname: McNamara
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D002460:Cell Line; D003094:Collagen / Q000378:metabolism; D005347:Fibroblasts / Q000201:enzymology* / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D020782:Matrix Metalloproteinases / Q000096:biosynthesis*; D008856:Microscopy, Fluorescence / Q000379:methods; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D011091:Polyesters / Q000737:chemistry; D011791:Quartz / Q000737:chemistry; D013499:Surface Properties; D013997:Time Factors
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; D011791:Quartz; C016240:polycaprolactone; D003094:Collagen; D020782:Matrix Metalloproteinases