title: Quantification of scar margin in keloid different from atrophic scar by multiphoton microscopic imaging.
abstract: Multiphoton microscopy (MPM) was applied to examine the marginal region at dermis of keloid compared with atrophic scar. High-resolution large-area image showed an obvious boundary at the scar margin and different morphological patterns of elastin and collagen on the two sides, further visualized by the focused three-dimensional images. Content alteration of elastin or collagen between the two sides of boundary was quantified to show significant difference between keloid and atrophic scar. Owing to the raised property of keloid with overproduced collagen on the scar side, the content alteration was positive for elastin and negative for collagen. On the contrary, the content alteration was negative for elastin and positive for collagen in the atrophic scar case due to the atrophic collagen on the scar side. It indicated that examination of the scar margin by MPM may lead a new way to discriminate different types of scars and better understand the scarring mechanisms.
authors.affiliation: Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou, China.
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Zhu
authors.forename: Shuangmu
authors.initials: S
authors.lastname: Zhuo
authors.forename: Liqin
authors.initials: L
authors.lastname: Zheng
authors.forename: Xingshan
authors.initials: X
authors.lastname: Jiang
authors.forename: Jianxin
authors.initials: J
authors.lastname: Chen
authors.forename: Bifang
authors.initials: B
authors.lastname: Lin
mesh_terms: D000328:Adult; D002921:Cicatrix / Q000145:classification* / Q000175:diagnosis / Q000473:pathology; D003094:Collagen / Q000378:metabolism; D020405:Dermis / Q000033:anatomy & histology / Q000473:pathology; D004549:Elastin / Q000378:metabolism; D005260:Female; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods; D007627:Keloid / Q000175:diagnosis* / Q000473:pathology; D008297:Male; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D012867:Skin / Q000473:pathology; D013518:Surgery, Plastic; D014945:Wound Healing
chemical_list: D003094:Collagen; D004549:Elastin