title: Polymorphisms of the cocaine-amphetamine-regulated transcript (CART) gene and their association with reproductive traits in Chinese goats.
abstract: Polymorphisms of the CART gene were investigated by PCR-single-strand conformation polymorphism analysis in 540 samples from 10 goat breeds. Ten novel single-nucleotide polymorphisms as well as three microsatellites were detected; a mutation, 77T → C, led to an amino acid change (Leu → Ser). Associations between polymorphic loci and reproductive traits were analyzed in Chuandong White, Guizhou White and Gulin Ma breeds. Mutation at position 524 had no significant effect on litter size in these three goat breeds. The polymorphism 539C → A differed significantly among the three breeds (P < 0.05); C(7)T(8)/C(9)T(8) at 939 was associated with larger litter size (P < 0.05) than genotypes C(7)T(8)/C(7)T(8) and C(7)T(8)/C(8)T(8). No significant association of birth weight was found with gene variation (524C → T, 539C → A and 939 CnTn). These findings could be valuable for marker-assisted selection for goat breeding.
authors.affiliation: College of Bioengineering, Chongqing University, Chongqing, PR. China. wang_pq@21cn.com
authors.forename: P Q
authors.initials: PQ
authors.lastname: Wang
authors.forename: L M
authors.initials: LM
authors.lastname: Deng
authors.forename: B Y
authors.initials: BY
authors.lastname: Zhang
authors.forename: M X
authors.initials: MX
authors.lastname: Chu
authors.forename: J Z
authors.initials: JZ
authors.lastname: Hou
mesh_terms: D000818:Animals; D001483:Base Sequence; D015415:Biomarkers; D001724:Birth Weight / Q000235:genetics; D001947:Breeding; D006041:Goats / Q000235:genetics*; D008098:Litter Size / Q000235:genetics; D018895:Microsatellite Repeats / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D018807:Polymorphism, Single-Stranded Conformational / Q000235:genetics*; D012098:Reproduction / Q000235:genetics*; D017422:Sequence Analysis, DNA; D000098882:Cocaine- and Amphetamine-Regulated Transcript Protein
chemical_list: D015415:Biomarkers; D009419:Nerve Tissue Proteins; D000098882:Cocaine- and Amphetamine-Regulated Transcript Protein