title: Genotoxic monitoring and benzene exposure assessment of gasoline station workers in metropolitan Bangkok: sister chromatid exchange (SCE) and urinary trans, trans-muconic acid (t,t-MA).
abstract: Early warning of the potential of mutagens or carcinogens caused by benzene exposure that might occur in gasoline station workers can be achieved by examining 2 major biomarkers: sister chromatid exchange (SCE) and trans, trans-muconic acid (t,t-MA), a urinary metabolite of benzene. The main objective of this study was to assess benzene exposure and monitor the genotoxic effect of gasoline station workers in Bangkok, Thailand. Blood and urine samples were collected from 33 gasoline station workers, working in Pathumwan district area, central Bangkok, Thailand, for SCE and t,t-MA analysis, from April to June 2009. Control samples were collected from 30 office workers and students in the same area at the same period. Our results indicated significantly higher frequencies of SCE in gasoline exposed workers were than in controls (p<0.01), independent of gender. Urinary t,t-MA and t,t-MA/creatinine levels of gasoline exposed workers were also significantly higher than the control groups (p<0.05) were significantly higher in women than men workers (p<0.01). Calculated chromosomal damage relative risk (RR) of gasoline station workers was 3.00 (95% CI = 1.81 - 4.98, p<0.001) compared to controls. The gasoline exposed workers had potentially higher risk of chromosomal damage and cancer development because of direct contact to benzene.
authors.affiliation: College of Public Health Sciences, Chulalongkorn University, Bangkok, Thailand. tkalayan@chula.ac.th
authors.forename: Tanasorn
authors.initials: T
authors.lastname: Tunsaringkarn
authors.forename: Jamsai
authors.initials: J
authors.lastname: Suwansaksri
authors.forename: Suphan
authors.initials: S
authors.lastname: Soogarun
authors.forename: Wattasit
authors.initials: W
authors.lastname: Siriwong
authors.forename: Anusorn
authors.initials: A
authors.lastname: Rungsiyothin
authors.forename: Kalaya
authors.initials: K
authors.lastname: Zapuang
authors.forename: Mark
authors.initials: M
authors.lastname: Robson
mesh_terms: D000328:Adult; D000395:Air Pollutants, Occupational / Q000506:poisoning; D001554:Benzene / Q000506:poisoning*; D014408:Biomarkers, Tumor / Q000032:analysis / Q000378:metabolism / Q000652:urine; D002273:Carcinogens; D003404:Creatinine / Q000652:urine; D003430:Cross-Sectional Studies; D004249:DNA Damage / Q000187:drug effects / Q000235:genetics; D004784:Environmental Monitoring / Q000379:methods; D005260:Female; D005742:Gasoline / Q000506:poisoning*; D006801:Humans; D008297:Male; D009784:Occupational Diseases / Q000139:chemically induced* / Q000235:genetics*; D016273:Occupational Exposure / Q000009:adverse effects* / Q000032:analysis; D012854:Sister Chromatid Exchange / Q000187:drug effects*; D013011:Sorbic Acid / Q000031:analogs & derivatives* / Q000378:metabolism; D013785:Thailand
chemical_list: D000395:Air Pollutants, Occupational; D014408:Biomarkers, Tumor; D002273:Carcinogens; D005742:Gasoline; C005939:muconic acid; D003404:Creatinine; D001554:Benzene; D013011:Sorbic Acid