title: Characterization of Defluviitalea saccharophila gen. nov., sp. nov., a thermophilic bacterium isolated from an upflow anaerobic filter treating abattoir wastewaters, and proposal of Defluviitaleaceae fam. nov.
abstract: A novel thermophilic, anaerobic, Gram-stain-positive, terminal-spore-forming bacterium was isolated from an upflow anaerobic filter treating abattoir wastewaters in Tunisia. This strain, designated LIND6LT2(T), grew at 40-60 °C (optimum 50-55 °C) and at pH 6.0-8.5 (optimum pH 7.0-7.5). It did not require NaCl for growth, but tolerated it up to 2%. Sulfate, thiosulfate, elemental sulfur, sulfite, nitrate and nitrite were not used as electron acceptors. Growth of LIND6LT2(T) was inhibited by sulfite (2 mM). Strain LIND6LT2(T) used cellobiose, glucose, mannose, maltose, mannitol, sucrose and xylose as electron donors. The main fermentation products from glucose metabolism were acetate, formate, butyrate and isobutyrate. The predominant cellular fatty acids were C(16:0) (68.4%) and C(14:0) (8.3%). The G+C content of the genomic DNA was 35.2 mol%. On the basis of its phylogenetic and physiological properties, a new genus and species, Defluviitalea saccharophila gen. nov., sp. nov., are proposed to accommodate strain LIND6LT2(T), placed in Defluviitaleaceae fam. nov. within the phylum Firmicutes, class Clostridia, order Clostridiales. Strain LIND6LT2(T) (=DSM 22681(T) =JCM 16312(T)) is the type strain of Defluviitalea saccharophila, which itself is the type species of Defluviitalea.
authors.affiliation: Laboratoire d'Ecologie et de Technologie Microbienne, Institut National des Sciences Appliquées et de Technologie, Centre Urbain Nord, BP 676, 1080 Tunis Cedex, Tunisia.|Université d'Aix-Marseille, Mediterranean Institute of Oceanography (MIO), IRD, MIOUMR 235, CNRS/INSU MIOUMR 7294, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France.
authors.forename: Linda
authors.initials: L
authors.lastname: Jabari
authors.affiliation: Laboratoire d'Ecologie et de Technologie Microbienne, Institut National des Sciences Appliquées et de Technologie, Centre Urbain Nord, BP 676, 1080 Tunis Cedex, Tunisia.|Université d'Aix-Marseille, Mediterranean Institute of Oceanography (MIO), IRD, MIOUMR 235, CNRS/INSU MIOUMR 7294, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France.
authors.forename: Hana
authors.initials: H
authors.lastname: Gannoun
authors.affiliation: Université d'Aix-Marseille, Mediterranean Institute of Oceanography (MIO), IRD, MIOUMR 235, CNRS/INSU MIOUMR 7294, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France.
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Cayol
authors.affiliation: Laboratoire d'Ecologie et de Technologie Microbienne, Institut National des Sciences Appliquées et de Technologie, Centre Urbain Nord, BP 676, 1080 Tunis Cedex, Tunisia.
authors.forename: Moktar
authors.initials: M
authors.lastname: Hamdi
authors.affiliation: Université d'Aix-Marseille, Mediterranean Institute of Oceanography (MIO), IRD, MIOUMR 235, CNRS/INSU MIOUMR 7294, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France.
authors.forename: Guy
authors.initials: G
authors.lastname: Fauque
authors.affiliation: Université d'Aix-Marseille, Mediterranean Institute of Oceanography (MIO), IRD, MIOUMR 235, CNRS/INSU MIOUMR 7294, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France.
authors.forename: Bernard
authors.initials: B
authors.lastname: Ollivier
authors.affiliation: Université d'Aix-Marseille, Mediterranean Institute of Oceanography (MIO), IRD, MIOUMR 235, CNRS/INSU MIOUMR 7294, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France.
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Fardeau
mesh_terms: D000003:Abattoirs; D015373:Bacterial Typing Techniques; D001482:Base Composition; D050260:Carbohydrate Metabolism; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005374:Filtration / Q000379:methods; D006094:Gram-Positive Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D008853:Microscopy; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D013171:Spores, Bacterial / Q000166:cytology; D013696:Temperature; D014416:Tunisia; D018508:Water Purification / Q000379:methods
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride