title: Comparative evaluation of PCR in Ziehl-Neelsen stained smears and PCR in tissues for diagnosis of Mycobacterium avium subsp. paratuberculosis.
abstract: Thirty six tissues from sheep, previously diagnosed with paratuberculosis, were tested by PCR in positive Ziehl-Neelsen staining smears of tissues, and PCR in tissues targeting IS900 specific for Mycobacterium avium subsp. paratuberculosis. DNA amplification was achieved in 33.3% Ziehl-Neelsen smears, and in 61.1% tissue samples. Combination of both techniques found 66.7% samples as positive. Combination of techniques would, therefore, increase the sensitivity of diagnosis.
authors.affiliation: Veterinary Microbiology Laboratory, Veterinary Medicine, Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro, 5001-801 Vila Real, Portugal. accoelho@utad.pt
authors.forename: A C
authors.initials: AC
authors.lastname: Coelho
authors.forename: M L
authors.initials: ML
authors.lastname: Pinto
authors.forename: A
authors.initials: A
authors.lastname: Miranda
authors.forename: A M
authors.initials: AM
authors.lastname: Coelho
authors.forename: M A
authors.initials: MA
authors.lastname: Pires
authors.forename: M
authors.initials: M
authors.lastname: Matos
mesh_terms: D000818:Animals; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D016927:Mycobacterium avium subsp. paratuberculosis / Q000235:genetics*; D009928:Organ Specificity; D010283:Paratuberculosis / Q000175:diagnosis* / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012756:Sheep / Q000382:microbiology; D013194:Staining and Labeling
chemical_list: D004269:DNA, Bacterial