title: In vitro and in vivo antioxidant properties of extracts from Coptis chinensis inflorescence.
abstract: The inflorescence of cultivated Coptis chinensis has been valued for tea production for many years in China. The antioxidant activities of C. chinensis inflorescence extracts prepared by various solvents were investigated by using several established in vitro systems: 2,2'-azinobis(3-ethylbenzthiazoline-6-sulphonic acid (ABTS), α,α-diphenyl-β-picrylhydrazyl (DPPH) and superoxide radical scavenging assays, reducing power assay, and ferrothiocyanate (FTC) and thiobarbituric acid (TBA) assays. The results showed that the 70% ethanol extract (EE) had the strongest antioxidant activity in vitro among the various extracts. Based on the in vitro results, EE was used to evaluate the antioxidant activity of C. chinensis inflorescence in vivo. The liver and kidney of intoxicated animals showed a significant decrease in superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) levels, while the malondialdehyde (MDA) level showed a significant increase. These changes were significantly reversed after treatment with EE and the standard vitamin E. Thus, the C. chinensis inflorescence may be a valuable natural source that can be applicable to food industries.
authors.affiliation: The State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, Peoples Republic of China.
authors.forename: Xiaoquan
authors.initials: X
authors.lastname: Ban
authors.forename: Bo
authors.initials: B
authors.lastname: Huang
authors.forename: Jingsheng
authors.initials: J
authors.lastname: He
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Chen
authors.forename: Hong
authors.initials: H
authors.lastname: Zeng
authors.forename: Li
authors.initials: L
authors.lastname: Han
authors.forename: Youwei
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D052160:Benzothiazoles / Q000737:chemistry / Q000378:metabolism; D001713:Biphenyl Compounds / Q000737:chemistry / Q000378:metabolism; D002374:Catalase / Q000187:drug effects / Q000378:metabolism; D002681:China; D039323:Coptis / Q000737:chemistry*; D004353:Drug Evaluation, Preclinical; D000431:Ethanol / Q000737:chemistry; D016166:Free Radical Scavengers / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D056626:Inflorescence / Q000737:chemistry; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D010851:Picrates / Q000737:chemistry / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D013451:Sulfonic Acids / Q000737:chemistry / Q000378:metabolism; D013482:Superoxide Dismutase / Q000187:drug effects / Q000378:metabolism; D013858:Thiobarbiturates / Q000737:chemistry / Q000378:metabolism; D014810:Vitamin E / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010851:Picrates; D010936:Plant Extracts; D013451:Sulfonic Acids; D013858:Thiobarbiturates; D014810:Vitamin E; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D000431:Ethanol; D008315:Malondialdehyde; C004931:1,1-diphenyl-2-picrylhydrazyl; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione; C029684:thiobarbituric acid