title: [Immunoregulatory function of interleukin-6 on human Th17 cells].
abstract: The aim of this study was to explore the regulatory function of interleukin-6(IL-6) on human Th17 cells. Human peripheral blood CD4(+) T cells were purified from healthy donors by anti-CD4 monoclonal antibody (mAb) conjugated microbeads. The experiment was divided into 2 groups. Test group in which CD4(+) T cells (1 × 10(6)/ml) were stimulated by human recombined IL-6 (20 ng/ml) for 4 days; control group in which CD4(+) T cells did not stimulated by IL-6. The concentrations of IL-17 protein in the supernatants were assayed by enzyme-linked immunosorbent assay (ELISA), and quantity of Th17 cells were detected by flow cytometry. The results showed that as compared to control group, IL-17 protein level in the supernatants of CD4(+) T cells significantly increased in IL-6 stimulated group: (337.05 ± 189.09 pg/ml; vs 15.07 ± 12.70 pg/ml) (p < 0.05). Furthermore, the percentage of Th17 cells in cultures of CD4(+) T cells stimulated by IL-6 was significantly higher than that in control group (4.05% ± 0.30% vs. 2.81% ± 0.44%)(p < 0.01). It is concluded that IL-6 promotes the expansion of Th17 cells in vitro.
authors.affiliation: Department of Hematology/Oncology, Tsinghua University First Hospital, Beijing, 100016, China. gzx2962@yahoo.com.cn
authors.forename: Zhen-Xing
authors.initials: ZX
authors.lastname: Guo
authors.forename: Cui-Ling
authors.initials: CL
authors.lastname: Zheng
authors.forename: Zhen-Ping
authors.initials: ZP
authors.lastname: Chen
authors.forename: Wen-Chuan
authors.initials: WC
authors.lastname: Dong
authors.forename: Ren-Chi
authors.initials: RC
authors.lastname: Yang
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology; D002478:Cells, Cultured; D006801:Humans; D015850:Interleukin-6 / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000276:immunology; D058504:Th17 Cells / Q000187:drug effects* / Q000276:immunology
chemical_list: D015850:Interleukin-6