title: Anti-oxidative and anti-genotoxic effects of methanolic extract of Mentha pulegium on human lymphocyte culture.
abstract: In the present work, methanolic extract of Mentha pulegium from Erzurum, Turkey, was used in order to report the results of anti-oxidant capacity, anti-oxidant activity and anti-genotoxic effects. The total antioxidant capacity and total phenolic content were measured by using CUPRAC, ABTS and Folin-Ciocalteu colorimetric methods. The total phenolic content was higher than the total antioxidant capacity (for the results of both the CUPRAC and ABTS methods) of methanolic extract of M pulegium (ME). Also, we evaluated the anti-oxidant enzyme activity such as superoxide dismutase (SOD) and glutation peroxidase, total glutation (GSH) and malondialdehyde (MDA) in human lymphocyte culture. In CCl₄-treated group, the activity of SOD, glutathione peroxidase (GPx) and GSH decreased significantly and the level of MDA increased significantly. A significant increase in the activity of SOD, GPx and the level of GSH were seen when supplemented with ME to CCl₄-treated group. Furthermore, a significant decrease in the level of MDA was observed when compared with CCl₄ alone treated group. In addition, anti-genotoxic effect of ME was studied by using sister chromatid exchange (SCE) method. As a result, ME has shown anti-genotoxic effect depend on anti-oxidative effect on human lymphocyte culture.
authors.affiliation: Department of Biology, Faculty of Art and Science, Fatih University, Istanbul, Turkey. lalpsoy@fatih.edu.tr.
authors.forename: Lokman
authors.initials: L
authors.lastname: Alpsoy
authors.forename: Hilal
authors.initials: H
authors.lastname: Sahin
authors.forename: Seyda
authors.initials: S
authors.lastname: Karaman
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D004249:DNA Damage / Q000187:drug effects; D005260:Female; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D006801:Humans; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000187:drug effects*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D031335:Mentha pulegium / Q000737:chemistry*; D000432:Methanol / Q000378:metabolism; D010636:Phenols / Q000032:analysis / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology*; D012854:Sister Chromatid Exchange; D013482:Superoxide Dismutase / Q000378:metabolism; D014421:Turkey
chemical_list: D000975:Antioxidants; D010636:Phenols; D010936:Plant Extracts; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione; D000432:Methanol