title: [Comparison of NO production level and proliferation of MCF-7 breast tumor cells under conditions of microenvironment modification].
abstract: Connection between the level of NO generation and its cytotoxic influence was investigated on two models of tumor cells (breast adenocarcinoma MCF-7) culture: monolayer and spheroids. Conditions of microenvironment were modified by Ca2+ channels blockers: EGTA, nitrendipine and Cl-lanthan. It was discovered in the work that deviation in concentration of extracellular Ca2+ under the influence of EGTA leads to reducing of the level of extracellular NO to 50% (P < or = 0.05) of control and increasing of the number of live cells by 10-40% (P < or = 0.05). Nitrendipin has the properties selective Ca2+ channels blocker that reduced the level of NO by 35-70% (P < or = 0.05), depending on the substances concentration and time of incubation. At that time proliferation potential of cells increased by 10-35% (P < or = 0.05). NO production in MCF-7 cells under the influence of Cl-lanthan in concentration of 0.01 and 0.1 mM is reduced by 50-60%, as compared with the control. But it had no influence on the number of live cells. On 2-D and 3-D models of tumor growth it was demonstrated that extracellular NO production in monolayer of MCF-7 was 3.2 pmol for 10(6) cells and for spheroids it was 8.5 pmol.
authors.forename: O M
authors.initials: OM
authors.lastname: Perepelytsina
authors.forename: L V
authors.initials: LV
authors.lastname: Harmanchuk
authors.forename: Ie A
authors.initials: IeA
authors.lastname: Shevchenko
authors.forename: M V
authors.initials: MV
authors.lastname: Sydorenko
authors.forename: L I
authors.initials: LI
authors.lastname: Ostapchenko
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology; D002118:Calcium / Q000378:metabolism; D002121:Calcium Channel Blockers / Q000494:pharmacology; D020746:Calcium Channels, L-Type / Q000378:metabolism; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003470:Culture Media; D005260:Female; D006801:Humans; D009569:Nitric Oxide / Q000096:biosynthesis*; D018874:Spheroids, Cellular / Q000378:metabolism / Q000473:pathology
chemical_list: D002121:Calcium Channel Blockers; D020746:Calcium Channels, L-Type; D003470:Culture Media; D009569:Nitric Oxide; D002118:Calcium