title: MED19 promotes proliferation and tumorigenesis of lung cancer.
abstract: MED19 is a subunit of Mediator that is an essential component of RNA polymerase II-mediated transcription machinery. High expression levels of MED19 were examined in human lung adenocarcinoma tissues by immunohistochemical assay. MED19-specific short hairpin RNA (shRNA) expressing lentivirus was constructed and infected lung cancer cell line A549. MED19 mRNA and protein expression levels were downregulated in A549 cells as evidenced by real-time PCR and western blot assays. Importantly, MED19 inhibition resulted in impaired proliferation and colony formation, and induced accumulation of G1-phase cells and mitigated invasiveness of cells. More importantly, downregulation of MED19 expression reduced the tumorigenicity of A549 cells in vivo. It was suggested that MED19 is a novel proliferation regulator that promotes growth of lung cancer cells, thereby indicating that MED19 may serve as a new molecular target for lung cancer therapy.
authors.affiliation: Department of Pathology, The Second Hospital of Jilin University, 218 Ziqiang Street, Changchun 130041, People's Republic of China.
authors.forename: Mei
authors.initials: M
authors.lastname: Sun
authors.forename: Rui
authors.initials: R
authors.lastname: Jiang
authors.forename: Jin-Dong
authors.initials: JD
authors.lastname: Li
authors.forename: Shu-Li
authors.initials: SL
authors.lastname: Luo
authors.forename: Hong-Wen
authors.initials: HW
authors.lastname: Gao
authors.forename: Cheng-Yan
authors.initials: CY
authors.lastname: Jin
authors.forename: Dong-Lei
authors.initials: DL
authors.lastname: Shi
authors.forename: Chun-Guang
authors.initials: CG
authors.lastname: Wang
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.forename: Xing-Yi
authors.initials: XY
authors.lastname: Zhang
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D016022:Case-Control Studies; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic; D005260:Female; D005822:Genetic Vectors; D006801:Humans; D016086:Lentivirus; D008175:Lung Neoplasms / Q000378:metabolism* / Q000473:pathology; D056892:Mediator Complex / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008819:Mice, Nude; D009361:Neoplasm Invasiveness / Q000235:genetics; D009368:Neoplasm Transplantation; D034622:RNA Interference; D047368:Tumor Burden / Q000235:genetics
chemical_list: C558319:MED19 protein, human; D056892:Mediator Complex