title: Bioconcentration and biotransformation of [¹⁴C]methoxychlor in the brackish water bivalve Corbicula japonica.
abstract: To obtain basic information on the metabolic fate of xenobiotics in the brackish water, bivalve Corbicula japonica, bioconcentration and biotransformation experiments were performed using methoxychlor (MXC) as a model compound. Bivalves were exposed to [ring-U-¹⁴C]MXC (10 µg L⁻¹) for 28 days under semi-static conditions followed by a 14-day depuration phase. The ¹⁴C concentration in the bivalves rapidly increased and reached a steady state after exposure for 7 days (BCFss = 2010); however, it rapidly decreased with a half-life of 2.2 days in the depuration phase. Mono- and bis-demethylated MXC, and their corresponding sulphate conjugates, were identified as minor metabolites. No glycoside conjugates (including glucuronide and glucoside) were detected. Despite this biotransformation system, bivalves were found to excrete retained MXC mostly unchanged although its relatively hydrophobic nature.
authors.affiliation: The Institute of Environmental Toxicology, Laboratory of Metabolism, Joso-shi, Japan. masuda@iet.or.jp
authors.forename: Minoru
authors.initials: M
authors.lastname: Masuda
authors.forename: Kazutoshi
authors.initials: K
authors.lastname: Ohyama
authors.forename: Osamu
authors.initials: O
authors.lastname: Hayashi
authors.forename: Koji
authors.initials: K
authors.lastname: Satsuma
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Sato
mesh_terms: D000818:Animals; D001711:Biotransformation; D002250:Carbon Radioisotopes; D002851:Chromatography, High Pressure Liquid; D049874:Corbicula / Q000378:metabolism*; D053858:Metabolic Networks and Pathways; D008731:Methoxychlor / Q000737:chemistry / Q000378:metabolism* / Q000493:pharmacokinetics*; D008745:Methylation; D021241:Spectrometry, Mass, Electrospray Ionization; D013237:Stereoisomerism; D014867:Water
chemical_list: D002250:Carbon Radioisotopes; D014867:Water; D008731:Methoxychlor