title: Performance evaluation of a modified chromogenic medium, ChromID MRSA New, for the detection of methicillin-resistant Staphylococcus aureus from clinical specimens.
abstract: A novel chromogenic medium for the detection of methicillin-resistant Staphylococcus aureus (MRSA), ChromID MRSA New, was evaluated and compared with the original ChromID MRSA agar, using 355 consecutive screening specimens from nose (120), throat (121) and perineum (114). The specimens were collected with an E-swab and inoculated within 24 hours onto both ChromID MRSA New and on ChromID MRSA. ChromID MRSA New was more sensitive than ChromID MRSA in detecting MRSA after 24 hours of incubation (94.3% versus 81.4%; p < 0.05). With the ChromID MRSA New, processing time is reduced from 48 h to 24 h and confirmation of the resistance to methicillin is redundant.
authors.affiliation: Department of Clinical Chemistry, Microbiology and Immunology, Ghent University Hospital, Ghent, Belgium. Frederik.VanHoecke@Ugent.be
authors.forename: F
authors.initials: F
authors.lastname: Van Hoecke
authors.forename: N
authors.initials: N
authors.lastname: Deloof
authors.forename: G
authors.initials: G
authors.lastname: Claeys
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D002353:Carrier State / Q000175:diagnosis / Q000382:microbiology; D002863:Chromogenic Compounds / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry*; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000302:isolation & purification*; D009666:Nose / Q000382:microbiology; D010502:Perineum / Q000382:microbiology; D010614:Pharynx / Q000382:microbiology; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000175:diagnosis* / Q000382:microbiology; D013997:Time Factors
chemical_list: D002863:Chromogenic Compounds; D003470:Culture Media