title: Microfluidic-based 18F-labeling of biomolecules for immuno-positron emission tomography.
abstract: Methods for tagging biomolecules with fluorine 18 as immuno-positron emission tomography (immunoPET) tracers require tedious optimization of radiolabeling conditions and can consume large amounts of scarce biomolecules. We describe an improved method using a digital microfluidic droplet generation (DMDG) chip, which provides computer-controlled metering and mixing of 18F tag, biomolecule, and buffer in defined ratios, allowing rapid scouting of reaction conditions in nanoliter volumes. The identified optimized conditions were then translated to bench-scale 18F labeling of a cancer-specific engineered antibody fragments, enabling microPET imaging of tumors in xenografted mice at 0.5 to 4 hours postinjection.
authors.affiliation: College of Electronics and Information Engineering, Wuhan Textile University, Wuhan, China.
authors.forename: Kan
authors.initials: K
authors.lastname: Liu
authors.forename: Eric J
authors.initials: EJ
authors.lastname: Lepin
authors.forename: Ming-Wei
authors.initials: MW
authors.lastname: Wang
authors.forename: Feng
authors.initials: F
authors.lastname: Guo
authors.forename: Wei-Yu
authors.initials: WY
authors.lastname: Lin
authors.forename: Yi-Chun
authors.initials: YC
authors.lastname: Chen
authors.forename: Shannon J
authors.initials: SJ
authors.lastname: Sirk
authors.forename: Sebastian
authors.initials: S
authors.lastname: Olma
authors.forename: Michael E
authors.initials: ME
authors.lastname: Phelps
authors.forename: Xing-Zhong
authors.initials: XZ
authors.lastname: Zhao
authors.forename: Hsian-Rong
authors.initials: HR
authors.lastname: Tseng
authors.forename: R
authors.initials: R
authors.lastname: Michael van Dam
authors.forename: Anna M
authors.initials: AM
authors.lastname: Wu
authors.forename: Clifton K-F
authors.initials: CK
authors.lastname: Shen
mesh_terms: D000818:Animals; D005462:Fluorine Radioisotopes / Q000493:pharmacokinetics; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007553:Isotope Labeling / Q000379:methods*; D008297:Male; D051379:Mice; D016513:Mice, SCID; D044085:Microfluidics / Q000379:methods*; D049268:Positron-Emission Tomography / Q000379:methods*; D017430:Prostate-Specific Antigen / Q000276:immunology*; D014018:Tissue Distribution; D014057:Tomography, X-Ray Computed
chemical_list: D005462:Fluorine Radioisotopes; D017430:Prostate-Specific Antigen