title: Development of an Aspergillus oryzae whole-cell biocatalyst coexpressing triglyceride and partial glyceride lipases for biodiesel production.
abstract: An Aspergillus oryzae whole-cell biocatalyst which coexpresses Fusarium heterosporum lipase (FHL) and mono- and di-acylglycerol lipase B (mdlB) in the same cell has been developed to improve biodiesel production. By screening a number of transformants, the best strain was obtained when FHL gene was integrated into A. oryzae chromosome using sC selection marker while mdlB was integrated using niaD selection marker. The reaction system using the lipase-coexpressing whole-cells was found to be superior in biodiesel production to others such as lipase-mixing and two-step reactions, affording the highest reaction rate and the highest ME content (98%). Moreover, an ME content of more than 90% was maintained during 10 repeated batch cycles. The whole-cell biocatalyst developed in this work would be promising biocatalysts for efficient biodiesel production.
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Adachi
authors.forename: Shinji
authors.initials: S
authors.lastname: Hama
authors.forename: Takao
authors.initials: T
authors.lastname: Numata
authors.forename: Kazunori
authors.initials: K
authors.lastname: Nakashima
authors.forename: Chiaki
authors.initials: C
authors.lastname: Ogino
authors.forename: Hideki
authors.initials: H
authors.lastname: Fukuda
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kondo
mesh_terms: D001236:Aspergillus oryzae / Q000378:metabolism*; D056804:Biofuels; D019149:Bioreactors; D002384:Catalysis; D002849:Chromatography, Gas; D005670:Fusarium / Q000201:enzymology; D008994:Monoacylglycerol Lipases / Q000378:metabolism*; D014280:Triglycerides / Q000378:metabolism*
chemical_list: D056804:Biofuels; D014280:Triglycerides; D008994:Monoacylglycerol Lipases