title: [Bioinformatics analysis of CO I gene of Simulium quinquestriatum].
abstract: The genomic DNA was extracted from Simulium quinquestriatum (Sq) and its CO I gene was amplified by PCR. The PCR product was purified and cloned into plasmid pMD18-T vector. The recombinant plasmid was transformed into Escherichia coli DH5alpha and then identified by digestion with restriction enzyme and PCR amplification. The amplified fractions (1 621 bp) included complete CO I gene (1 542 bp, GenBank accession number: DQ534949), 5' tRNA-Tyr and 3' tRNA-Leu partial fraction. The CO I gene sequence had a high identity (99%) with that of S. quinquestriatum (GenBank accession number: AY251520). Bioinformatics analysis showed that the Sq-CO I open reading frame encoded a 513-amino acid protein with M(r) 5565, pI5.84. Structural prediction showed this protein possessed a conservative domain of CO I gene.
authors.affiliation: Department of Biochemistry, Medical School, Hubei University for Nationalities, Enshi 445000, China.
authors.forename: Hong-Bin
authors.initials: HB
authors.lastname: Luo
authors.forename: Ming
authors.initials: M
authors.lastname: Yang
authors.forename: Han-Bin
authors.initials: HB
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D019295:Computational Biology; D003576:Electron Transport Complex IV / Q000235:genetics*; D008969:Molecular Sequence Data; D012843:Simuliidae / Q000145:classification / Q000201:enzymology / Q000235:genetics*
chemical_list: D003576:Electron Transport Complex IV