title: [Study on the expression and the role of macrophage migration inhibitory factor in the brain of neonatal rats with hypoxia-ischemia brain damage].
abstract: OBJECTIVE
  To explore the expression of macrophage migration inhibitory factor (MIF), phosphorylated extracellular signal regulated kinases 1/2 (pERK1/2), matrix metalloproteinase-9 (MMP-9) in brain and the correlations with cerebral edema after hypoxia-ischemia brain damage (HIBD).
  
  
  METHODS
  144 seven-day-old SD rats were randomly dividend into three groups that was the sham operation group, HIBD group and intervention group with anti-MIF. Each group were executed in 1 h, 6 h, 12 h and 24 h, 3 d, and 7 d. The expression of MIF, MMP-9 protein in the right cerebral cortex was detected with immunohistochemistry methods, the relative content of pERK1/2 was detected with Western blot and the water content of brain was evaluated with dry-wet method.
  
  
  RESULTS
  Comparing with the sham group the expression of MIF in the HIBD group began to increase on 1 h, and peaked on 24 h (P < 0.05), and MMP-9 began to express on 6 h, and also peaked on 24 h (P < 0.05), while pERK1/2 expressed rapidly to peak on 1 h, and quickly decreased on 6 h, and began to increase on 12 h and reached the peak again on 24 h (P < 0.05). Being treated with hypoxia-ischemia, the water content of brain began to increase on 6 h and peaked on 3 d (P < 0.05). And comparing with HIBD group, the expressions of MIF, pERK1/2, MMP-9 and the water contents were decreased obviously in the anti-MIF group (P < 0.05).
  
  
  CONCLUSIONS
  The increased expression of MIF in the neonatal rats with hypoxia-ischemia brain damage maybe via activing the ERK1/2 ways to induce MMP-9 expressand leading to cerebral edema.
authors.affiliation: Department of Neonatal, West China Second Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Li-li
authors.initials: LL
authors.lastname: Wang
authors.forename: Wen-jun
authors.initials: WJ
authors.lastname: Guo
authors.forename: Ying
authors.initials: Y
authors.lastname: Xiong
authors.forename: Wei-na
authors.initials: WN
authors.lastname: Du
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001929:Brain Edema / Q000209:etiology*; D005260:Female; D020925:Hypoxia-Ischemia, Brain / Q000150:complications / Q000378:metabolism*; D019746:Intramolecular Oxidoreductases / Q000378:metabolism*; D020935:MAP Kinase Signaling System; D008263:Macrophage Migration-Inhibitory Factors / Q000378:metabolism*; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D008263:Macrophage Migration-Inhibitory Factors; D020780:Matrix Metalloproteinase 9; D019746:Intramolecular Oxidoreductases; C515599:Mif protein, rat