title: Ammonia disinfection of hatchery waste for elimination of single-stranded RNA viruses.
abstract: Hatchery waste, an animal by-product of the poultry industry, needs sanitation treatment before further use as fertilizer or as a substrate in biogas or composting plants, owing to the potential presence of opportunistic pathogens, including zoonotic viruses. Effective sanitation is also important in viral epizootic outbreaks and as a routine, ensuring high hygiene standards on farms. This study examined the use of ammonia at different concentrations and temperatures to disinfect hatchery waste. Inactivation kinetics of high-pathogenic avian influenza virus H7N1 and low-pathogenic avian influenza virus H5N3, as representatives of notifiable avian viral diseases, were determined in spiked hatchery waste. Bovine parainfluenza virus type 3, feline coronavirus, and feline calicivirus were used as models for other important avian pathogens, such as Newcastle disease virus, infectious bronchitis virus, and avian hepatitis E virus. Bacteriophage MS2 was also monitored as a stable indicator. Coronavirus was the most sensitive virus, with decimal reduction (D) values of 1.2 and 0.63 h after addition of 0.5% (wt/wt) ammonia at 14 and 25°C, respectively. Under similar conditions, high-pathogenic avian influenza H7N1 was the most resistant, with D values of 3.0 and 1.4 h. MS2 was more resistant than the viruses to all treatments and proved to be a suitable indicator of viral inactivation. The results indicate that ammonia treatment of hatchery waste is efficient in inactivating enveloped and naked single-stranded RNA viruses. Based on the D values and confidence intervals obtained, guidelines for treatment were proposed, and one was successfully validated at full scale at a hatchery, with MS2 added to hatchery waste.
authors.affiliation: Department of Virology, Immunobiology and Parasitology, National Veterinary Institute (SVA), SE-751 89 Uppsala, Sweden. Eva.Emmoth@sva.se
authors.forename: Eva
authors.initials: E
authors.lastname: Emmoth
authors.forename: Jakob
authors.initials: J
authors.lastname: Ottoson
authors.forename: Ann
authors.initials: A
authors.lastname: Albihn
authors.forename: Sándor
authors.initials: S
authors.lastname: Belák
authors.forename: Björn
authors.initials: B
authors.lastname: Vinnerås
mesh_terms: D000641:Ammonia / Q000633:toxicity*; D000822:Animal Husbandry; D000818:Animals; D004202:Disinfectants / Q000633:toxicity*; D004203:Disinfection / Q000379:methods*; D050296:Microbial Viability / Q000187:drug effects*; D011200:Poultry; D012328:RNA Viruses / Q000187:drug effects* / Q000502:physiology; D012499:Sanitation / Q000379:methods; D013696:Temperature
chemical_list: D004202:Disinfectants; D000641:Ammonia