title: Poly-beta-hydroxybutyrate production by Pseudomonas sp. RZS 1 under aerobic and semi-aerobic condition.
abstract: Pseudomonas sp. RZS1 was isolated from distillery effluent and identified based on phenotypic characters and 16s rRNA sequencing. It accumulated optimum amount (703.79 microg/mg of biomass) of poly-beta-hydroxybutyrate (PHB) under aerobic process of fermentation and 75 microg/mg of biomass under the anaerobic process of fermentation. Aerobic fermentation yielded 9.3-fold more PHB than semi-aerobic fermentation. Acetone alcohol method proved to be the best suitable recovery method as it gave 703.79 microg PHB per mg of biomass with a percentage recovery yield of 70.37. It started to accumulate PHB at the end of lag phase (from 6 h of incubation). Optimum amount of PHB (20 microg/ml) was reported during early stationary phase (30 h of incubation). Extracted PHB showed two peaks, minor one at 248 nm and major one at 365 nm. IR spectra revealed the presence of functional groups characteristics of PHB.
authors.affiliation: PG Department of Microbiology, S. I. Patil Arts, G. B. Patel Science and S.T.S.K.V.S. Commerce College, Shahada, Dist. Nandurbar 425409, India. sayyedrz@gmail.com
authors.forename: R Z
authors.initials: RZ
authors.lastname: Sayyed
authors.forename: N S
authors.initials: NS
authors.lastname: Gangurde
mesh_terms: D000332:Aerobiosis; D006885:Hydroxybutyrates / Q000378:metabolism*; D010641:Phenotype; D011091:Polyesters / Q000378:metabolism*; D011549:Pseudomonas / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017550:Spectroscopy, Fourier Transform Infrared; D013997:Time Factors; D014865:Waste Disposal, Fluid; D000094122:Polyhydroxybutyrates
chemical_list: D006885:Hydroxybutyrates; D011091:Polyesters; D012336:RNA, Ribosomal, 16S; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates