title: Production of extracellular polysaccharide by Bacillus megaterium RB-05 using jute as substrate.
abstract: Bacillus megaterium RB-05 was grown on glucose and on "tossa-daisee" (Corchorus olitorius)-derived jute, and production and composition of extracellular polysaccharide (EPS) were monitored. An EPS yield of 0.065 ± 0.013 and of 0.297 g ± 0.054 g(-1) substrate after 72 h was obtained for glucose and jute, respectively. EPS production in the presence of jute paralleled bacterial cellulase activity. High performance liquid chromatography (HPLC), matrix assisted LASER desorption/ionization-time of flight (MALDI-ToF) mass spectroscopy, and fourier transform infrared (FT-IR) spectroscopy demonstrated that the EPS synthesized in jute culture (JC) differed from that synthesized in glucose mineral salts medium (GMSM). While fucose was only a minor constituent (4.9 wt.%) of EPS from GMSM, it a major component (41.9 wt.%) of EPS synthesized in JC. This study establishes jute as an effective fermentation substrate for EPS production by a cellulase-producing bacterium.
authors.affiliation: Materials Science Centre, Indian Institute of Technology Kharagpur, West Bengal 721302, India.
authors.forename: Sougata Roy
authors.initials: SR
authors.lastname: Chowdhury
authors.forename: Ratan Kumar
authors.initials: RK
authors.lastname: Basak
authors.forename: Ramkrishna
authors.initials: R
authors.lastname: Sen
authors.forename: Basudam
authors.initials: B
authors.lastname: Adhikari
mesh_terms: D001410:Bacillus megaterium / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D002480:Cellulase / Q000378:metabolism; D032381:Corchorus / Q000378:metabolism*; D005110:Extracellular Space / Q000378:metabolism*; D009005:Monosaccharides / Q000032:analysis; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*
chemical_list: D009005:Monosaccharides; D011135:Polysaccharides, Bacterial; D002480:Cellulase