title: Apoptosis photoinduction by C60 fullerene in human leukemic T cells.
abstract: The viability of normal (Wistar rat thymocytes) and transformed (human leukemia Jurkat cells) T cells after UV/Vis irradiation in the presence of pristine C60 fullerene was studied. The data obtained have shown that C60 fullerene exhibits cytotoxic effect against transformed T lymphocytes when combined with UV/Vis irradiation using mercury-vapor lamp (320-600 nm). C60 fullerene photocytotoxicity was not detected in thymocytes. C60-dependent photoinduced apoptosis of Jurkat cells was confirmed by DNA fragmentation and caspase-3 activation. No substantial increase of caspase-3 activation was observed in thymocytes treated with C60 fullerene plus irradiation, while antileukemic agent cytosine arabinoside was shown to induce caspase-3 activation both in Jurkat cells and thymocytes. The data obtained may be useful for development of photosensitizers for photodynamic therapy with selective action on leukemia cells.
authors.affiliation: Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv.
authors.forename: K O
authors.initials: KO
authors.lastname: Palyvoda
authors.forename: I I
authors.initials: II
authors.lastname: Grynyuk
authors.forename: S V
authors.initials: SV
authors.lastname: Prylutska
authors.forename: A A
authors.initials: AA
authors.lastname: Samoylenko
authors.forename: L B
authors.initials: LB
authors.lastname: Drobot
authors.forename: O P
authors.initials: OP
authors.lastname: Matyshevska
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D015153:Blotting, Western; D053148:Caspase 3 / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D053938:DNA Fragmentation / Q000187:drug effects / Q000528:radiation effects; D005260:Female; D037741:Fullerenes / Q000494:pharmacology*; D006801:Humans; D019169:Jurkat Cells; D008027:Light; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013601:T-Lymphocytes / Q000187:drug effects / Q000473:pathology / Q000528:radiation effects; D013950:Thymus Gland / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D013997:Time Factors
chemical_list: D037741:Fullerenes; D017319:Photosensitizing Agents; D053148:Caspase 3; C069837:fullerene C60