title: Molecular identification of `Candidatus Mycoplasma haemovis' in sheep with hemolytic anemia.
abstract: We examined the presence of hemoplasmas, hemotropic mycoplasmas, among 11 sheep (Ovis aries) with regenerative and hemolytic anemia and found six of them were positive by real-time PCR. The positive samples were then subjected to conventional PCR for direct sequencing of the 16S rRNA gene. Nucleotide sequences of all the positive samples were identified as the 16S rRNA gene of `Candidatus Mycoplasma haemovis' by phylogenetic analysis, demonstrating the infections with this particular hemoplasma species in Japan.
authors.affiliation: Department of Veterinary Microbiology, Faculty of Agriculture, Iwate University, Morioka 020–8550, Japan.
authors.forename: Jin
authors.initials: J
authors.lastname: Suzuki
authors.forename: Fumina
authors.initials: F
authors.lastname: Sasaoka
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Fujihara
authors.forename: Yusaku
authors.initials: Y
authors.lastname: Watanabe
authors.forename: Tomoko
authors.initials: T
authors.lastname: Tasaki
authors.forename: Shinichi
authors.initials: S
authors.lastname: Oda
authors.forename: Saori
authors.initials: S
authors.lastname: Kobayashi
authors.forename: Reeko
authors.initials: R
authors.lastname: Sato
authors.forename: Kazuya
authors.initials: K
authors.lastname: Nagai
authors.forename: Ryô
authors.initials: R
authors.lastname: Harasawa
mesh_terms: D000743:Anemia, Hemolytic / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D000818:Animals; D004196:Disease Outbreaks / Q000662:veterinary*; D007564:Japan / Q000453:epidemiology; D009174:Mycoplasma / Q000235:genetics / Q000302:isolation & purification*; D009175:Mycoplasma Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012329:RNA, Bacterial / Q000097:blood; D012336:RNA, Ribosomal, 16S / Q000097:blood; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D017423:Sequence Analysis, RNA / Q000662:veterinary; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology*
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S