title: Rice OsGL1-1 is involved in leaf cuticular wax and cuticle membrane.
abstract: Cuticular wax forms a hydrophobic barrier on aerial plant organs; it plays an important role in protecting a plant from damage caused by many forms of environmental stress. In the present study, we characterized a rice leaf wax-deficient mutant osgl1-1 derived from a spontaneous mutation, which exhibited a wax-deficient and highly hydrophilic leaf phenotype. We cloned the OsGL1-1 gene by the map-based cloning method and performed a complementation test to confirm the function of the candidate gene. Molecular studies revealed that OsGL1-1 was a member of the OsGL1 family, and contained regions that were homologous to some regions in sterol desaturases and short-chain dehydrogenases/reductases. Compared to the wild-type, the osgl1-1 mutant showed decreased cuticular wax deposition, thinner cuticular membrane, decreased chlorophyll leaching, increased rate of water loss, and enhanced sensitivity to drought. OsGL1-1 is expressed ubiquitously in rice. The transient expression of OsGL1-1-green fluorescent protein fusion protein indicated that OsGL1-1 is localized in the cytoplasm, plasma membrane, and nucleus.
authors.affiliation: State Key Laboratory of Plant Genomics and Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Bao-Xiang
authors.initials: BX
authors.lastname: Qin
authors.forename: Ding
authors.initials: D
authors.lastname: Tang
authors.forename: Jian
authors.initials: J
authors.lastname: Huang
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.forename: Xin-Ru
authors.initials: XR
authors.lastname: Wu
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Lu
authors.forename: Ke-Jian
authors.initials: KJ
authors.lastname: Wang
authors.forename: Heng-Xiu
authors.initials: HX
authors.lastname: Yu
authors.forename: Jian-Min
authors.initials: JM
authors.lastname: Chen
authors.forename: Ming-Hong
authors.initials: MH
authors.lastname: Gu
authors.forename: Zhu-Kuan
authors.initials: ZK
authors.lastname: Cheng
mesh_terms: D021581:Active Transport, Cell Nucleus; D000595:Amino Acid Sequence; D002467:Cell Nucleus / Q000378:metabolism; D003001:Cloning, Molecular; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D008566:Membranes / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D012275:Oryza / Q000166:cytology* / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D010539:Permeability; D018515:Plant Leaves / Q000166:cytology* / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D014885:Waxes / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D014885:Waxes