title: Organic and conventional Coffea arabica L.: a comparative study of the chemical composition and physiological, biochemical and toxicological effects in Wistar rats.
abstract: Differentiation between organic and conventional coffee has increased due to the growing demand and high consumption of healthy foods that contain compounds with antioxidant potential, which have been associated with the reduction of chronic diseases. We used organic and conventional coffee in powder 4% (w/w) and infusions 5%, 10% and 20% (w/v) incorporated in a commercial diet to test in vivo. The levels of chlorogenic acid, caffeine and trigonelline were determined by high performance liquid chromatography. The body weight, weight gain, food consumption, aberrant foci crypt, mucin depleted foci, stress biomarkers protein carbonyl and malondialdehyde, biochemical parameters and behavior of the rats were compared between the experimental and control groups within a framework of colon carcinogenesis. The organic coffee showed higher levels of chlorogenic acid, caffeine and trigonelline than conventional, however, this difference did not significantly affect behavior. The infusions had an antioxidant effect, reducing the levels of malondialdehyde; however, the biochemical parameters of the serum were not altered, and there was neither induction nor prevention of preneoplasic lesions.
authors.affiliation: Department of Exact Science, Federal University of Alfenas-MG, Rua Gabriel Monteiro da Silva, 700, CEP: 37130-000, Alfenas, MG, Brazil. dayenemg@yahoo.com.br
authors.forename: Dayene do Carmo
authors.initials: Ddo C
authors.lastname: Carvalho
authors.forename: Maisa Ribeiro Pereira Lima
authors.initials: MR
authors.lastname: Brigagão
authors.forename: Marcelo Henrique
authors.initials: MH
authors.lastname: dos Santos
authors.forename: Fernanda Borges Araújo
authors.initials: FB
authors.lastname: de Paula
authors.forename: Alexandre
authors.initials: A
authors.lastname: Giusti-Paiva
authors.forename: Luciana
authors.initials: L
authors.lastname: Azevedo
mesh_terms: D000470:Alkaloids / Q000032:analysis* / Q000494:pharmacology; D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D001522:Behavior, Animal / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D002110:Caffeine / Q000032:analysis* / Q000494:pharmacology; D002726:Chlorogenic Acid / Q000032:analysis* / Q000494:pharmacology; D040503:Coffea / Q000737:chemistry*; D003106:Colon / Q000187:drug effects / Q000473:pathology; D003110:Colonic Neoplasms / Q000517:prevention & control; D004305:Dose-Response Relationship, Drug; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009077:Mucins / Q000187:drug effects; D058871:Organic Agriculture; D018384:Oxidative Stress / Q000187:drug effects; D011230:Precancerous Conditions; D050050:Protein Carbonylation / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000470:Alkaloids; D016588:Anticarcinogenic Agents; D000975:Antioxidants; D009077:Mucins; D002726:Chlorogenic Acid; D002110:Caffeine; C009560:trigonelline; D008315:Malondialdehyde