title: Large-area protein patterns generated by ordered binary colloidal assemblies as templates.
abstract: We demonstrate the use of binary colloidal assemblies as lithographic masks to generate tunable Au patterns on SiO(2) substrates with dimensions ranging from micrometers to nanometers. Such patterns can be modified with different chemistries to create patterns with well-defined sites for selective adsorption of proteins, where the pattern size and spacing is adjustable depending on particle choice. In our system, the binary colloidal assemblies contain large and small particles of similar or different material and are self-assembled from dilute dispersions with particle size ratios ranging from 0.10 to 0.50. This allows masks with variable morphology and thus production of chemical patterns of tunable geometry. Finally, the Au or SiO(2) regions of the pattern are surface modified with protein resistant oligoethyleneglycol self-assembled molecules, which facilitates site selective adsorption of proteins into the unmodified regions of the pattern. This we show with fluorescently labeled bovine serum albumin.
authors.affiliation: Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Ny Munkegade, Building 1521, 8000 Aarhus C, Denmark.
authors.forename: Gurvinder
authors.initials: G
authors.lastname: Singh
authors.forename: Vipul
authors.initials: V
authors.lastname: Gohri
authors.forename: Saju
authors.initials: S
authors.lastname: Pillai
authors.forename: Ayyoob
authors.initials: A
authors.lastname: Arpanaei
authors.forename: Morten
authors.initials: M
authors.lastname: Foss
authors.forename: Peter
authors.initials: P
authors.lastname: Kingshott
mesh_terms: D000327:Adsorption; D003102:Colloids / Q000737:chemistry; D003460:Crystallization / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D046911:Macromolecular Substances / Q000737:chemistry; D008422:Materials Testing; D008567:Membranes, Artificial; D008968:Molecular Conformation; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D010316:Particle Size; D011506:Proteins / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D003102:Colloids; D046911:Macromolecular Substances; D008567:Membranes, Artificial; D011506:Proteins; D006046:Gold