title: [A method for differential identification of group C and G streptococci with PCR].
abstract: The paper describes a rapid method for PCR identification of Groups C and G streptococci (Streptococcus dysgalactiae subspecies equisimilis, Streptococcus constellatus, and Streptococcus anginosus) that cause human disease. Species-specific regions of the cpn60 gene encoding heat shock protein GroEL (HSP60) were chosen as markers for PCR diagnosis; three pairs of primers were constructed for these regions, each of which was peculiar to the specific type. The method was tested on a large collection of pathogenic streptococci of different serogroups isolated from man and animals; its specificity was shown to identify S. dysgalactiae subsp. equisimilis, S. constellatus, and S. anginosus. The proposed method has all benefits of PCR-based techniques, which enables it to be used for the purposes of molecular epidemiology.
authors.forename: Iu Iu
authors.initials: IuIu
authors.lastname: Il'iasov
authors.forename: I
authors.initials: I
authors.lastname: Biswas
authors.forename: A A
authors.initials: AA
authors.lastname: Totolian
authors.forename: A V
authors.initials: AV
authors.lastname: Dmitriev
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D006801:Humans; D016133:Polymerase Chain Reaction; D013291:Streptococcus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D034366:Streptococcus anginosus / Q000235:genetics / Q000302:isolation & purification; D034365:Streptococcus constellatus / Q000235:genetics / Q000302:isolation & purification