title: Polymorphisms of the CYP1A1, CYP2E1 and XRCC1 genes and cancer risk in a Southern Italian population: a case-control study.
abstract: BACKGROUND
  Polymorphisms in genes encoding enzymes involved in xenobiotic metabolism and/or in cellular defenses against carcinogen-induced DNA damage play an important role in determining individual cancer susceptibility. However, their distribution and association with cancer susceptibility can vary in different populations.
  
  
  METHODS
  A case-control study including 290 cancer patients (cases) and 242 controls was performed to evaluate the relationship between polymorphisms of cytochrome P450 (CYP)1A1 and CYP2E1 and X-ray repair complementing defective repair in Chinese hamster cells (XRCC)1 genes and the risk of developing cancer in a Southern Italian (Basilicata) population. Genomic DNA was isolated from 5 ml whole blood and genotyping was performed using a PCR-RFLP technique.
  
  
  RESULTS
  No significant differences were observed in the distribution of the CYP1A1, CYP2E1 and XRCC1 gene polymorphisms between the cases and controls in the population under study.
  
  
  CONCLUSIONS
  The distribution of CYP1A1, CYP2E1 and XRCC1 gene polymorphisms in the Basilicata population is not different from that of other Italian regions or from that reported in the literature for Caucasian populations, and polymorphisms in these genes do not play an important role in determining cancer risk in the population under study.
authors.affiliation: Istituto di Ricovero e Cura a Carattere Scientifico, Rionero in Vulture, Potenza, Italy.
authors.forename: Gabriella
authors.initials: G
authors.lastname: Bianchino
authors.forename: Achille
authors.initials: A
authors.lastname: Cittadini
authors.forename: Vitina
authors.initials: V
authors.lastname: Grieco
authors.forename: Antonio
authors.initials: A
authors.lastname: Traficante
authors.forename: Angela
authors.initials: A
authors.lastname: Zupa
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Improta
authors.forename: Michele
authors.initials: M
authors.lastname: Aieta
authors.forename: Alessandro
authors.initials: A
authors.lastname: Sgambato
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics*; D019392:Cytochrome P-450 CYP2E1 / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D006801:Humans; D007558:Italy / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000453:epidemiology* / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D000076105:X-ray Repair Cross Complementing Protein 1
chemical_list: D004273:DNA, Neoplasm; D004268:DNA-Binding Proteins; D000076105:X-ray Repair Cross Complementing Protein 1; C000619371:XRCC1 protein, human; D019392:Cytochrome P-450 CYP2E1; D019363:Cytochrome P-450 CYP1A1