title: A difference gel electrophoresis study on thylakoids isolated from poplar leaves reveals a negative impact of ozone exposure on membrane proteins.
abstract: Populus tremula L. x P. alba L. (Populus x canescens (Aiton) Smith), clone INRA 717-1-B4, saplings were subjected to 120 ppb ozone exposure for 28 days. Chloroplasts were isolated, and the membrane proteins, solubilized using the detergent 1,2-diheptanoyl-sn-glycero-3-phosphocholine (DHPC), were analyzed in a difference gel electrophoresis (DiGE) experiment comparing control versus ozone-exposed plants. Extrinsic photosystem (PS) proteins and adenosine triphosphatase (ATPase) subunits were detected to vary in abundance. The general trend was a decrease in abundance, except for ferredoxin-NADP(+) oxidoreductase (FNR), which increased after the first 7 days of exposure. The up-regulation of FNR would increase NAPDH production for reducing power and detoxification inside and outside of the chloroplast. Later on, FNR and a number of PS and ATPase subunits decrease in abundance. This could be the result of oxidative processes on chloroplast proteins but could also be a way to down-regulate photochemical reactions in response to an inhibition in Calvin cycle activity.
authors.affiliation: Department Environment and Agro-biotechnologies, CRP-Gabriel Lippmann, 41 rue du Brill, L-4422 Belvaux, GD, Luxembourg. sachabo@pt.lu
authors.forename: Sacha
authors.initials: S
authors.lastname: Bohler
authors.forename: Kjell
authors.initials: K
authors.lastname: Sergeant
authors.forename: Lucien
authors.initials: L
authors.lastname: Hoffmann
authors.forename: Pierre
authors.initials: P
authors.lastname: Dizengremel
authors.forename: Jean-Francois
authors.initials: JF
authors.lastname: Hausman
authors.forename: Jenny
authors.initials: J
authors.lastname: Renaut
authors.forename: Yves
authors.initials: Y
authors.lastname: Jolivet
mesh_terms: D003902:Detergents / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D005287:Ferredoxin-NADP Reductase / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D009249:NADP / Q000096:biosynthesis*; D010084:Oxidation-Reduction / Q000187:drug effects; D010126:Ozone / Q000009:adverse effects* / Q000494:pharmacology; D010742:Phospholipid Ethers / Q000737:chemistry; D010788:Photosynthesis / Q000187:drug effects / Q000235:genetics*; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D032107:Populus / Q000235:genetics / Q000378:metabolism*; D025341:Principal Component Analysis; D040901:Proteomics / Q000379:methods*; D015398:Signal Transduction / Q000187:drug effects; D020524:Thylakoids / Q000235:genetics / Q000378:metabolism
chemical_list: C052298:1,2-dihexadecyl-sn-glycero-3-phosphocholine; D003902:Detergents; D010742:Phospholipid Ethers; D010940:Plant Proteins; D009249:NADP; D010126:Ozone; D005287:Ferredoxin-NADP Reductase