title: Nicotine induces pro-inflammatory response in aortic vascular smooth muscle cells through a NFκB/osteopontin amplification loop-dependent pathway.
abstract: Nicotine has anti- and pro-inflammatory properties in various cells. Its role in aortic vascular smooth muscle cells (VSMC) was explored. Human aortic VSMC were cultured. After nicotine (1.0 μM) and/or pyrrolidinedithiocarbamic acid (PDTC, 50 μM) treatment, the activation of nuclear factor κB (NFκB) was investigated. The levels of pro-inflammatory cytokines, osteopontin (OPN), interleukin-6 (IL-6), and monocyte chemoattractant protein 1 (MCP-1) were also assessed. After OPN was downregulated by small interfering RNA (siRNA) transfection, the pro-inflammatory effect was reassessed. We found that NFκB was activated after nicotine administration. Nicotine upregulated OPN, IL-6, and MCP-1 expressions, and this effect attenuated after PDTC pretreatment. RNAi knocked down the OPN expression in nicotine-treated cells and abolished its pro-inflammatory effects. We concluded that nicotine induces a pro-inflammatory response in VSMC through a NFκB/osteopontin amplification loop-dependent pathway.
authors.affiliation: Department of Cardiovascular Surgery, RenJi Hospital of Shanghai Jiaotong University, Shanghai, 200127, People's Republic of China.
authors.forename: Yongyi
authors.initials: Y
authors.lastname: Wang
authors.forename: Fei
authors.initials: F
authors.lastname: Zhang
authors.forename: Wengang
authors.initials: W
authors.lastname: Yang
authors.forename: Song
authors.initials: S
authors.lastname: Xue
mesh_terms: D001011:Aorta / Q000378:metabolism*; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000096:biosynthesis; D006801:Humans; D007249:Inflammation / Q000378:metabolism; D015850:Interleukin-6 / Q000096:biosynthesis; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism*; D009538:Nicotine / Q000494:pharmacology*; D053495:Osteopontin / Q000378:metabolism*; D011759:Pyrrolidines / Q000494:pharmacology; D034622:RNA Interference; D034741:RNA, Small Interfering; D012907:Smoking / Q000009:adverse effects; D013859:Thiocarbamates / Q000494:pharmacology
chemical_list: D018932:Chemokine CCL2; D015850:Interleukin-6; D016328:NF-kappa B; D011759:Pyrrolidines; D034741:RNA, Small Interfering; D013859:Thiocarbamates; D053495:Osteopontin; C020972:pyrrolidine dithiocarbamic acid; D009538:Nicotine