title: MiD49 and MiD51, new components of the mitochondrial fission machinery.
abstract: Mitochondria form intricate networks through fission and fusion events. Here, we identify mitochondrial dynamics proteins of 49 and 51 kDa (MiD49 and MiD51, respectively) anchored in the mitochondrial outer membrane. MiD49/51 form foci and rings around mitochondria similar to the fission mediator dynamin-related protein 1 (Drp1). MiD49/51 directly recruit Drp1 to the mitochondrial surface, whereas their knockdown reduces Drp1 association, leading to unopposed fusion. Overexpression of MiD49/51 seems to sequester Drp1 from functioning at mitochondria and cause fused tubules to associate with actin. Thus, MiD49/51 are new mediators of mitochondrial division affecting Drp1 action at mitochondria.
authors.affiliation: La Trobe Institute for Molecular Science, La Trobe University, Plenty Road, Melbourne 3086, Victoria, Australia.
authors.forename: Catherine S
authors.initials: CS
authors.lastname: Palmer
authors.forename: Laura D
authors.initials: LD
authors.lastname: Osellame
authors.forename: David
authors.initials: D
authors.lastname: Laine
authors.forename: Olga S
authors.initials: OS
authors.lastname: Koutsopoulos
authors.forename: Ann E
authors.initials: AE
authors.lastname: Frazier
authors.forename: Michael T
authors.initials: MT
authors.lastname: Ryan
mesh_terms: D000199:Actins / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D002460:Cell Line; D002522:Chlorocebus aethiops; D006367:HeLa Cells; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism* / Q000473:pathology; D051336:Mitochondrial Membranes / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010445:Peptide Elongation Factors / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport / Q000235:genetics; D034622:RNA Interference; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D014475:Uncoupling Agents / Q000494:pharmacology
chemical_list: D000199:Actins; C561316:MIEF1 protein, human; C000608253:MIEF2 protein, mouse; D008565:Membrane Proteins; C000597830:MiD51 protein, mouse; D008869:Microtubule-Associated Proteins; D024101:Mitochondrial Proteins; D010445:Peptide Elongation Factors; D018160:Receptors, Cytoplasmic and Nuclear; D014475:Uncoupling Agents; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone