title: Selection dynamic of Escherichia coli host in M13 combinatorial peptide phage display libraries.
abstract: Phage display relies on an iterative cycle of selection and amplification of random combinatorial libraries to enrich the initial population of those peptides that satisfy a priori chosen criteria. The effectiveness of any phage display protocol depends directly on library amino acid sequence diversity and the strength of the selection procedure. In this study we monitored the dynamics of the selective pressure exerted by the host organism on a random peptide library in the absence of any additional selection pressure. The results indicate that sequence censorship exerted by Escherichia coli dramatically reduces library diversity and can significantly impair phage display effectiveness.
authors.affiliation: European Centre for Living Technology, Ca' Foscari University Venice, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Zanconato
authors.forename: Giovanni
authors.initials: G
authors.lastname: Minervini
authors.forename: Irene
authors.initials: I
authors.lastname: Poli
authors.forename: Davide
authors.initials: D
authors.lastname: De Lucrezia
mesh_terms: D017104:Bacteriophage M13 / Q000235:genetics*; D020650:Combinatorial Chemistry Techniques; D004926:Escherichia coli / Q000166:cytology / Q000235:genetics*; D019151:Peptide Library
chemical_list: D019151:Peptide Library