title: Ecofriendly decolorisation of Cr-complex dye Acid Black 172 by a newly isolated Pseudomonas sp. strain DY1.
abstract: Pseudomonas sp. strain DY1 was newly isolated from soil with rotten wood and identified as a member of the genus Pseudomonas based on 16S rDNA and biochemical tests. Acid Black 172, a water soluble Cr-complex dye, was then selected as a model dye to investigate the decolorisation ability of the strain. It was observed that the growth of the strain was not inhibited by high dose of metal ions (10 mM), and efficient decolorisation was still observed when high concentrations of Fe(2+), Fe(3+) and Ca(2+) existed. The optimal decolorising conditions obtained from Taguchi design were as follows: temperature 37˚C, pH 7.0, Fe(3+) and proline concentrations of 7 mM and 3.0 g/L, respectively. Under the optimal conditions, 94.5% of Acid Black 172 (100 mg/L) could be decolorised by the strain in 24 h. The kinetics of the decolorisation best fitted the first order kinetic model (R(2)=0.981). Besides, the phytotoxicity study demonstrated a good detoxification by the strain. This work has a certain practical value in microbial decolorisation of textile wastewater.
authors.affiliation: College of Life Science Zhejiang University, 310058, Hangzhou, Zhejiang Province, China.
authors.forename: Lin-Na
authors.initials: LN
authors.lastname: Du
authors.forename: Sheng
authors.initials: S
authors.lastname: Wang
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.forename: Yu-Yi
authors.initials: YY
authors.lastname: Yang
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Jia
authors.forename: Yu-Hua
authors.initials: YH
authors.lastname: Zhao
mesh_terms: D001673:Biodegradation, Environmental; D004396:Coloring Agents / Q000378:metabolism / Q000633:toxicity; D056831:Coordination Complexes / Q000378:metabolism* / Q000633:toxicity; D005163:Factor Analysis, Statistical; D009282:Naphthalenesulfonates / Q000378:metabolism* / Q000633:toxicity; D010802:Phylogeny; D011549:Pseudomonas / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S; D013783:Textile Industry; D014865:Waste Disposal, Fluid / Q000379:methods*; D014871:Water Microbiology; D018508:Water Purification / Q000379:methods*
chemical_list: D004396:Coloring Agents; D056831:Coordination Complexes; D009282:Naphthalenesulfonates; D012336:RNA, Ribosomal, 16S; C557641:acid black 172