title: Immuno-enzymatic evaluation of the recombinant TSSA-II protein of Trypanosoma cruzi in dogs and human sera: a tool for epidemiological studies.
abstract: The rTSSA-II (recombinant Trypomastigote Small Surface II) antigen was evaluated by ELISA to detect anti-Trypanosoma cruzi antibodies in sera from naturally infected dogs and humans. For this evaluation ELISA-rTSSA-II was standardized and groups were classified according to the results obtained through xenodiagnosis, ELISA and PCR. Sensitivity (Se), Specificity (Sp), Kappa index (KI) and area under curve (AUC) were determined. The Se was determined by using 14 sera from dogs infected with T. cruzi VI (TcVI) whereas Sp was determined by using 95 non-chagasic sera by xenodiagnosis, ELISA-Homogenate and PCR. The performance of ELISA-rTSSA-II in dog sera was high (AUC=0·93 and KI=0·91). The Se was 92·85% (1 false negative) and Sp was 100%. Two sera from dogs infected with TcI and 1 with TcIII were negative. For patients infected with T. cruzi, reactivity was 87·8% (36/41), there was only 1 indeterminate, and Sp was 100%. Fifty-four sera from non-chagasic and 68 sera from patients with cutaneous leishmaniasis did not react with rTSS-II. ELISA-rTSSA-II showed a high performance when studying sera from naturally infected dogs and it also presented 100% Sp. This assay could be an important tool to carry out sero-epidemiological surveys on the prevalence of T. cruzi circulating lineages in the region.
authors.affiliation: Cátedra de Química Biológica, Facultad de Ciencias Naturales. Universidad Nacional de Salta, Av. Bolivia 5150, Salta (4400), Argentina. rcimino@unsa.edu.ar
authors.forename: R O
authors.initials: RO
authors.lastname: Cimino
authors.forename: M Monje
authors.initials: MM
authors.lastname: Rumi
authors.forename: P
authors.initials: P
authors.lastname: Ragone
authors.forename: J
authors.initials: J
authors.lastname: Lauthier
authors.forename: A Alberti
authors.initials: AA
authors.lastname: D'Amato
authors.forename: I R López
authors.initials: IR
authors.lastname: Quiroga
authors.forename: J F
authors.initials: JF
authors.lastname: Gil
authors.forename: S P
authors.initials: SP
authors.lastname: Cajal
authors.forename: N
authors.initials: N
authors.lastname: Acosta
authors.forename: M
authors.initials: M
authors.lastname: Juárez
authors.forename: A
authors.initials: A
authors.lastname: Krolewiecki
authors.forename: V
authors.initials: V
authors.lastname: Orellana
authors.forename: R
authors.initials: R
authors.lastname: Zacca
authors.forename: I
authors.initials: I
authors.lastname: Marcipar
authors.forename: P
authors.initials: P
authors.lastname: Diosque
authors.forename: J R
authors.initials: JR
authors.lastname: Nasser
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D000953:Antigens, Protozoan / Q000276:immunology / Q000302:isolation & purification; D000954:Antigens, Surface / Q000276:immunology / Q000302:isolation & purification; D001118:Argentina / Q000453:epidemiology; D014355:Chagas Disease / Q000175:diagnosis* / Q000453:epidemiology / Q000469:parasitology; D004283:Dog Diseases / Q000175:diagnosis* / Q000453:epidemiology / Q000469:parasitology; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D006801:Humans; D011994:Recombinant Proteins / Q000276:immunology / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D016036:Seroepidemiologic Studies; D014349:Trypanosoma cruzi / Q000276:immunology*
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D000954:Antigens, Surface; D011994:Recombinant Proteins