title: Comparative analysis of enzymatic stability and amino acid sequences of thermostable alkaline proteases from two haloalkaliphilic bacteria isolated from Coastal region of Gujarat, India.
abstract: Thermostable alkaline proteases from two haloalkaliphilic bacteria, Oceanobacillus iheyensis O.M.A(1)8 (EU680961) and Haloalkaliphilic bacterium O.M.E(1)2 (EU680960) were studied for enzymatic properties and amino acid sequences in comparative manner. The bacteria were isolated from salt enriched soil located in Okha, Coastal Gujarat, India. The unique aspect of the study was that alkaline protease from Haloalkaliphilic bacterium O.M.A(1)8 optimally catalyzed the reaction over a wide range of temperature, 50-90°C, with a half-life of 36 h at 90°C. The molecular weights of O.M.A(1)8 and O.M.E(1)2 were 35 kDa and 25 kDa, respectively. The enzyme secretion was over the broader range of pH 8-11, with an optimum at 11. The alkaline proteases from the two haloalkaliphilic strains isolated from the same site reflected quite different characteristics features. To the best of our knowledge, we have not come across with any such report on the thermal stability of alkaline proteases from haloalkaliphiles. Amino acid sequences for both enzymes were deduced from the nucleotide sequences of their corresponding genes followed by the analysis of physico-chemical properties of the enzymes.
authors.affiliation: Department of Biosciences, Saurashtra University, Rajkot 360005, India.
authors.forename: Megha K
authors.initials: MK
authors.lastname: Purohit
authors.forename: Satya P
authors.initials: SP
authors.lastname: Singh
mesh_terms: D000595:Amino Acid Sequence; D001406:Bacillaceae / Q000201:enzymology*; D001483:Base Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D004795:Enzyme Stability / Q000235:genetics; D006207:Half-Life; D006863:Hydrogen-Ion Concentration; D007194:India; D007700:Kinetics; D008969:Molecular Sequence Data; D012697:Serine Endopeptidases / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D012988:Soil Microbiology; D013696:Temperature
chemical_list: D012697:Serine Endopeptidases; C032923:microbial serine proteinases