title: Effect of long-term normobaric hyperoxia on oxidative stress in mitochondria of the guinea pig brain.
abstract: Normobaric hyperoxia (NBO) is applied for treatment of various clinical conditions related to hypoxia, but it can potentially also induce generation of reactive oxygen species, causing cellular damage. In this study, we examined the effects of 60 h NBO treatment on lipid and protein oxidative damage and activity of superoxide dismutase (Mn-SOD) in brain mitochondria of guinea pigs. Despite significant stimulation of Mn-SOD expression and activity the NBO treatment resulted in accumulation of markers of oxidative lesions, including lipid peroxidation (conjugated dienes, thiobarbituric acid reactive substances) and protein modification (bityrosines, adducts with lipid peroxidation products, oxidized thiols). When inhaled O(2) was enriched with oxygen cation, O (2) (•+) , the Mn-SOD expression and activity were stimulated to similar extend, but lipid peroxidation and protein oxidation were prevented. These results suggest that long-term NBO treatment causes oxidative stress, but enrichment of inhaled oxygen by oxygen cation can protect the brain again adverse effects of hyperoxia.
authors.affiliation: Department of Medical Biochemistry, Jessenius Faculty of Medicine, Comenius University, Mala Hora 4, 036 01, Martin, Slovak Republic.
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Tatarkova
authors.forename: Ivan
authors.initials: I
authors.lastname: Engler
authors.forename: Andrea
authors.initials: A
authors.lastname: Calkovska
authors.forename: Daniela
authors.initials: D
authors.lastname: Mokra
authors.forename: Anna
authors.initials: A
authors.lastname: Drgova
authors.forename: Peter
authors.initials: P
authors.lastname: Hodas
authors.forename: Jan
authors.initials: J
authors.lastname: Lehotsky
authors.forename: Dusan
authors.initials: D
authors.lastname: Dobrota
authors.forename: Peter
authors.initials: P
authors.lastname: Kaplan
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism*; D006168:Guinea Pigs; D018496:Hyperoxia; D015227:Lipid Peroxidation; D008297:Male; D008928:Mitochondria / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D010100:Oxygen / Q000494:pharmacology*; D013385:Succinate Dehydrogenase / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D017392:Thiobarbituric Acid Reactive Substances; D013482:Superoxide Dismutase; D013385:Succinate Dehydrogenase; D010100:Oxygen