title: On the use of Kinetex(™) -C(18) core-shell 2.6 µm stationary phase to the multiclass determination of antibiotics.
abstract: Fast and accurate analysis is a prerequisite in all analytical fields especially in food, biological, pharmaceutical, and environmental samples. The new trend of ultra performance liquid chromatography (LC) has the main drawback of expensive instrumentation, which can't be easily found in low-budget analytical laboratories. The evolution of core shell technology has contributed to this direction, since ultra high efficiency can be achieved on common LC instrument platforms. Herein the novel core shell analytical column, KINETEX (™) 2.6 µm, (150 mm × 4.6 mm) was comparatively studied against two conventional reversed-phase silica-based and one monolithic column. Eight antimicrobial agents representing two different classes: penicillins and amphenicols, were separated using a typical 400 bar high performance liquid chromatography (HPLC) equipment. Comparison of column performance was carried out by calculation of the number of theoretical plates N, the tailing factor T(f) , the relative retention time RRT, the retention factor k, the resolution factor R(s) , and the precision of the retention time and peak area. Optimal chromatographic conditions were used to validate the method. Its applicability was proven by the analysis of veterinary drug formulations. The examined antibiotics were well resolved within 17 min. Limit of quantitation values were 25.9 ng for amoxicillin, 14.1 ng for ampicillin, 41.6 ng for thiamphenicol, 9.6 ng for oxacillin, 23.5 ng for florfenicol, 26.7 for cloxacillin, 23.5 ng for chloramphenicol and 42.3 ng for dicloxacillin for 20 µL injection volume. The developed method can be easily and readily transferred to any laboratory.
authors.affiliation: Laboratory of Analytical Chemistry, Department of Chemistry, Aristotle University of Thessaloniki, Thessaloniki, 54124 Greece. samanidu@chem.auth.gr
authors.forename: Victoria F
authors.initials: VF
authors.lastname: Samanidou
authors.forename: Eftichia G
authors.initials: EG
authors.lastname: Karageorgou
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis*; D002244:Carbon / Q000592:standards*; D002851:Chromatography, High Pressure Liquid / Q000379:methods / Q000592:standards; D015203:Reproducibility of Results; D012117:Resins, Synthetic / Q000592:standards*; D019155:Veterinary Drugs / Q000032:analysis*
chemical_list: D000900:Anti-Bacterial Agents; D012117:Resins, Synthetic; D019155:Veterinary Drugs; D002244:Carbon