title: Insulin signaling in adipose tissue of patients with primary aldosteronism.
abstract: OBJECTIVE
  We studied phosphorylation of insulin-receptors substrate downstream molecules: 1) in the ex-vivo visceral adipose tissue (VAT) of patients with aldosterone-producing adenoma (APA) (no.=7) and non-functioning adenoma (NFA) (no.=7) undergoing laparoscopic adrenalectomy; 2) in aldosterone-treated sc adipocytes of subjects (no.=5) who requested abdominoplasty.
  
  
  METHODS
  Western blotting was used to detect phosphorylation of Akt and extracellular signal-regulated kinase (ERK) 1/2 in VAT from APA and NFA patients, and in subcutaneous adipocytes pre-treated with different aldosterone concentrations. Phosphorylation of Akt and ERK1/2 was similar in VAT of patients with APA and NFA. Pre-treatment in adipocytes with both physiological (1 nM) and pharmacological (10 μM) doses of aldosterone did not affect basal or insulin-induced phosphorylation of Akt and ERK1/2.
  
  
  CONCLUSIONS
  Our data give further evidence that insulin signaling in human VAT is not affected by primary aldosterone overproduction.
authors.affiliation: Department of Medical and Surgical Sciences, University of Padova, Via Giustiniani 2, 35128 Padova, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Urbanet
authors.forename: C
authors.initials: C
authors.lastname: Pilon
authors.forename: F
authors.initials: F
authors.lastname: Giorgino
authors.forename: R
authors.initials: R
authors.lastname: Vettor
authors.forename: F
authors.initials: F
authors.lastname: Fallo
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism*; D000306:Adrenal Cortex Neoplasms / Q000473:pathology / Q000503:physiopathology / Q000601:surgery; D000315:Adrenalectomy; D018246:Adrenocortical Adenoma / Q000473:pathology / Q000503:physiopathology / Q000601:surgery; D000328:Adult; D000368:Aged; D000450:Aldosterone / Q000097:blood / Q000494:pharmacology; D002478:Cells, Cultured; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005260:Female; D006801:Humans; D006929:Hyperaldosteronism / Q000503:physiopathology* / Q000601:surgery; D007328:Insulin / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*
chemical_list: D007328:Insulin; D000450:Aldosterone; D051057:Proto-Oncogene Proteins c-akt; D048049:Extracellular Signal-Regulated MAP Kinases