title: Combined cross-linked enzyme aggregates from versatile peroxidase and glucose oxidase: production, partial characterization and application for the elimination of endocrine disruptors.
abstract: Versatile peroxidase (VP) from Bjerkandera adusta was insolubilized in the form of cross-linked enzyme aggregates (CLEA®s). Of the initially applied activity 67% was recovered as CLEA®s. Co-aggregation of VP with glucose oxidase from Aspergillus niger led to an increased activity recovery of 89%. The combined CLEA®s showed higher stability against H(2)O(2) and exerted VP activity upon glucose addition. The elimination of the endocrine disrupting chemicals bisphenol A, nonylphenol, triclosan, 17α-ethinylestradiol and the hormone 17β-estradiol (10 mg L(-1) each) and the removal of their estrogenic activity by combined CLEA®s were tested in batch experiments. Within 10 min, the combined CLEA®s were able to remove all the endocrine disruptors except triclosan (residual concentration 74%). The removal of the estrogenic activity was higher than 55% for all compounds, except triclosan. A membrane reactor continuously operated with combined CLEA®s could almost completely remove bisphenol A (10 mg L(-1)) for 43 h.
authors.affiliation: Department of Chemical Engineering, University of Santiago de Compostela, E-15782 Santiago de Compostela, Spain. roberto.taboada@rai.usc.es
authors.forename: R
authors.initials: R
authors.lastname: Taboada-Puig
authors.forename: C
authors.initials: C
authors.lastname: Junghanns
authors.forename: P
authors.initials: P
authors.lastname: Demarche
authors.forename: M T
authors.initials: MT
authors.lastname: Moreira
authors.forename: G
authors.initials: G
authors.lastname: Feijoo
authors.forename: J M
authors.initials: JM
authors.lastname: Lema
authors.forename: S N
authors.initials: SN
authors.lastname: Agathos
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology*; D001559:Benzhydryl Compounds; D001673:Biodegradation, Environmental; D019149:Bioreactors; D055453:Coriolaceae / Q000201:enzymology*; D003432:Cross-Linking Reagents / Q000378:metabolism*; D052244:Endocrine Disruptors / Q000302:isolation & purification*; D005949:Glucose Oxidase / Q000378:metabolism*; D007700:Kinetics; D008345:Manganese / Q000378:metabolism; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000737:chemistry* / Q000378:metabolism*; D010636:Phenols / Q000302:isolation & purification; D020836:Protein Structure, Quaternary; D011748:Pyrogallol / Q000031:analogs & derivatives / Q000378:metabolism; D013379:Substrate Specificity; D013997:Time Factors; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D003432:Cross-Linking Reagents; D052244:Endocrine Disruptors; D005949:Glucose Oxidase; D008345:Manganese; D009195:Peroxidase; D010636:Phenols; D011748:Pyrogallol; C010120:pyrogallol 1,3-dimethyl ether; C006780:bisphenol A; D000098826:Bisphenol A Compounds