title: Fundamental study of the radiation monitoring system based on evaluation of DNA lesions.
abstract: The biological dosemeter that measures biological responses to ionising radiation is useful for radiation protection. This paper presents the development and characterisation of a gamma ray irradiation dosimetry system based on real-time PCR (polymerase chain reaction) methodology. Real-time PCR is used to amplify and simultaneously quantify a targeted DNA molecule. If there are no limitations due to limiting substrates or reagents, at each extension step, the amount of DNA target is doubled, leading to exponential (geometric) amplification of the specific DNA fragment. The essential point of this assay is that DNA lesions caused by ionising radiation block DNA synthesis by DNA polymerase, resulting in a decrease in the amplification of a damaged DNA template compared with that of non-damaged DNA templates.
authors.affiliation: Radioisotope Research Center, Osaka University, Yamada-oka 2-4, Suita, Osaka 565-0871, Japan. shimizu@rirc.osaka-u.ac.jp
authors.forename: Kikuo
authors.initials: K
authors.lastname: Shimizu
authors.forename: Youichirou
authors.initials: Y
authors.lastname: Matuo
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Izumi
authors.forename: Toshiji
authors.initials: T
authors.lastname: Ikeda
mesh_terms: D003037:Cobalt Radioisotopes; D004247:DNA / Q000528:radiation effects*; D004249:DNA Damage / Q000528:radiation effects*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D005720:Gamma Rays / Q000009:adverse effects*; D006801:Humans; D016133:Polymerase Chain Reaction; D011834:Radiation Monitoring
chemical_list: D003037:Cobalt Radioisotopes; D004247:DNA; D004259:DNA-Directed DNA Polymerase