title: A new Beta defensin from sika deer: molecular cloning and sequence characterization.
abstract: The beta-defensins are small, well-characterized peptides with broad antimicrobial activities. Here we report the identification of a novel β-defensin, sika deer β-defensin-1 (siBD-1), from sika deer tissues with a pair of PCR primers according to the conserved cDNA sequences of known ruminant β-defensins. Total RNA was extracted from the tongue epithelia of a sika deer and the 418 bp cDNA encoding siBD-1 was amplified by the reverse transcription PCR (RT-PCR), 5'- and 3'-RACE. The cDNA contained an open reading frame (ORF) of 192 bases which encoded a 64 amino acid prepro-peptide. The prepro-peptide contained six invariantly spaced cysteine residues, which is the β defensin consensus sequence. The putative mature peptide of the siBD-1 contained nine positively charged residues (5 arginine-R, 3 lysine-K, and 1 histidine-H). The sequence homology shows that siBD-1 has 73.0-90.6% amino-acid identity and 74.6-90.6% cDNA identity with other ruminant beta-defensins, sharing the greatest identity with buffalo enteric β-defensin in both amino acid and nucleotide sequences.
authors.affiliation: Veterinary Medicine College of Inner Mongolia Agricultural University, Hohhot City, China.
authors.forename: Yinfeng
authors.initials: Y
authors.lastname: Yang
authors.forename: Lan
authors.initials: L
authors.lastname: Jing
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.forename: Guifang
authors.initials: G
authors.lastname: Cao
authors.forename: Shuying
authors.initials: S
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002020:Buffaloes / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D003670:Deer / Q000235:genetics* / Q000276:immunology*; D008297:Male; D012313:RNA / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D023083:beta-Defensins / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D023083:beta-Defensins; D012313:RNA