title: Narciclasine modulates polar auxin transport in Arabidopsis roots.
abstract: Plant development displays an exceptional plasticity and adaptability that involves the dynamic, asymmetric distribution of the phytohormone auxin. Polar auxin flow, which requires transport facilitators of the PIN family, largely contributes to the establishment and maintenance of auxin gradients and mediates multiple developmental processes. Here, we report the effects of narciclasine (NCS), an Amaryllidaceae alkaloid isolated from Narcissus tazetta bulbs, on postembryonic development of Arabidopsis roots. Arabidopsis seedlings grown on NCS showed defects in root gravitropism which correlates with a reduction in auxin transport in roots. Expressions of auxin transport genes were affected and the polar localization of PIN2 protein was altered under NCS treatment. Taken together, we propose that NCS modulates auxin transport gene expression and PIN2 localization, and thus affects auxin transport and auxin distribution necessary for postembryonic development of Arabidopsis roots.
authors.affiliation: School of Life Sciences, Lanzhou University, Lanzhou 730000, People's Republic of China.
authors.forename: Xiaofan
authors.initials: X
authors.lastname: Na
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Hu
authors.forename: Kun
authors.initials: K
authors.lastname: Yue
authors.forename: Hongxia
authors.initials: H
authors.lastname: Lu
authors.forename: Pengfei
authors.initials: P
authors.lastname: Jia
authors.forename: Huahua
authors.initials: H
authors.lastname: Wang
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Wang
authors.forename: Yurong
authors.initials: Y
authors.lastname: Bi
mesh_terms: D047151:Amaryllidaceae Alkaloids / Q000494:pharmacology*; D017360:Arabidopsis / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D001692:Biological Transport; D018506:Gene Expression Regulation, Plant; D018522:Gravitropism / Q000187:drug effects*; D007210:Indoleacetic Acids / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D010617:Phenanthridines / Q000494:pharmacology*; D010937:Plant Growth Regulators / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D036226:Seedlings / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism
chemical_list: D047151:Amaryllidaceae Alkaloids; D029681:Arabidopsis Proteins; D007210:Indoleacetic Acids; D026901:Membrane Transport Proteins; D010617:Phenanthridines; D010937:Plant Growth Regulators; C116363:PIN1 protein, Arabidopsis; C117013:AT5G57090 protein, Arabidopsis; C010753:narciclasine