title: A new subspecies of Anoxybacillus flavithermus ssp. yunnanensis ssp. nov. with very high ethanol tolerance.
abstract: In a search for thermophilic ethanol-tolerant bacteria, water-sediment samples collected at springs in Yunnan province of China were screened by ethanol enrichment. A novel thermophilic bacterium, strain E13(T) , was isolated. It exhibits a unique and remarkable ability to preferably grow in the presence of ethanol and is able to tolerate 13% (v/v) ethanol at 60 °C. The isolate is a facultative aerobic, Gram-positive, motile, spore-forming rod that is capable of utilizing a range of carbon sources, such as xylose, arabinose and cellobiose. Phylogenetic analysis based on 16S rRNA gene similarity showed the strain to be affiliated with the species Anoxybacillus flavithermus (99.2% sequence similarity). DNA-DNA hybridization comparisons demonstrated a 64.8% DNA-DNA relatedness between strain E13(T) and A. flavithermus DSM 2641(T) . On the basis of phenotypic characteristics, phylogenetic data and DNA-DNA hybridization data, it was concluded that the isolate merited classification as a novel subspecies of A. flavithermus, for which the name Anoxybacillus flavithermus ssp. yunnanensis ssp. nov. is proposed. The type strain of this subspecies is E13(T) (=CCTCC AB2010187(T) =KCTC 13759(T) ).
authors.affiliation: Engineering Technology Research Center of Microorganisms and Biocatalysis, Anhui Province, School of Life Sciences, Anhui University, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Dai
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Yin
authors.initials: Y
authors.lastname: Lei
authors.forename: Yi
authors.initials: Y
authors.lastname: Gao
authors.forename: Fang
authors.initials: F
authors.lastname: Han
authors.forename: Yazhong
authors.initials: Y
authors.lastname: Xiao
authors.forename: Hui
authors.initials: H
authors.lastname: Peng
mesh_terms: D058677:Anoxybacillus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D002681:China; D000431:Ethanol / Q000378:metabolism*; D019015:Geologic Sediments / Q000382:microbiology; D045482:Hot Springs / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny
chemical_list: D000431:Ethanol