title: Endogenous hydrogen peroxide up-regulates the expression of nitric oxide synthase in the kidney of SHR.
abstract: METHODS
  Both nitric oxide synthase (NOS) expression and oxidative stress are elevated in the tissues of spontaneously hypertensive rats (SHR) compared with Wistar-Kyoto rats (WKY). The purpose of the present study was to determine the relationship between the endothelial and neuronal NOS (eNOS and nNOS) expression and oxidative stress in the kidney of SHR and WKY.
  
  
  RESULTS
  Plasma and urinary hydrogen peroxide (H₂O₂) and nitrate/nitrite (NOx), the renal NADPH oxidase activity and eNOS and nNOS expressions were all higher in SHR than in WKY. Although the treatment with either the NADPH oxidase inhibitor, apocynin or the superoxide dismutase mimetic, tempol for 8 weeks decreased the systolic blood pressure (SBP) and inhibited the renal NADPH oxidase activity in SHR, apocynin decreased but tempol increased the plasma and urinary H₂O₂ and NOx and the eNOS and nNOS expressions in the renal cortex and medulla of SHR. In contrast to SHR, neither apocynin nor tempol affected these parameters in WKY. H₂O₂ administered intravenously for 1 week in WKY increased plasma and urinary H₂O₂ and NOx and the eNOS and nNOS expressions in the renal cortex and medulla in a dose-dependent manner without changing the renal NADPH oxidase activity.
  
  
  CONCLUSIONS
  These results indicate that oxidative stress up-regulates the NOS expression in the kidney of SHR compared with WKY; and that endogenous H₂O₂ is a mediator of the up-regulation of the NOS expression in the kidney of SHR.
authors.affiliation: Department of Internal Medicine and Rehabilitation Science, Japan.
authors.forename: Pengyu
authors.initials: P
authors.lastname: Cao
authors.forename: Osamu
authors.initials: O
authors.lastname: Ito
authors.forename: Qi
authors.initials: Q
authors.lastname: Guo
authors.forename: Daisuke
authors.initials: D
authors.lastname: Ito
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Muroya
authors.forename: Rong
authors.initials: R
authors.lastname: Rong
authors.forename: Takefumi
authors.initials: T
authors.lastname: Mori
authors.forename: Sadayoshi
authors.initials: S
authors.lastname: Ito
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kohzuki
mesh_terms: D000818:Animals; D006861:Hydrogen Peroxide / Q000378:metabolism*; D007668:Kidney / Q000201:enzymology*; D008297:Male; D019001:Nitric Oxide Synthase / Q000235:genetics*; D018384:Oxidative Stress; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D015854:Up-Regulation / Q000187:drug effects*
chemical_list: D006861:Hydrogen Peroxide; D019001:Nitric Oxide Synthase