title: Cyclotron production of radioactive CeO(2) nanoparticles and their application for in vitro uptake studies.
abstract: Nowadays, a wide variety of nanoparticles (NPs) are applied in different fields such as medical science and industry. Due to their large commercial volume, the OECD Working Party on Manufactured Nanomaterials (NMs) has proposed to study a set of 14 nanomaterials, one of which being cerium oxide (CeO(2)). In particular, CeO(2) based NPs are widely used in automotive industry, healthcare, and cosmetics. In this paper, we propose a method for the production of radioactive CeO(2) NPs.We demonstrate that they maintain the same physicochemical characteristics as the “cold” ones in terms of size distribution and Zeta potential; we develop a new protocol to assess their cellular interaction in immortalized mouse fibroblast cell line Balb/3T3, a model for the study of basal cytotoxicity and carcinogenic potential induced by chemicals and in the present case by NPs. Experimental result of this work, which shows a quasi-linear concentration-uptake response of cells, can be useful as a reference dose-uptake curve for explaining effects following biological uptake after exposure to CeO(2) NPs.
authors.affiliation: Institute for Health and Consumer Protection, Joint Research Centre, European Commission, Via E. Fermi 2749, I-21020 Ispra (VA), Italy. federica.simonelli@jrc.ec.europa.eu
authors.forename: Federica
authors.initials: F
authors.lastname: Simonelli
authors.forename: P
authors.initials: P
authors.lastname: Marmorato
authors.forename: K
authors.initials: K
authors.lastname: Abbas
authors.forename: J
authors.initials: J
authors.lastname: Ponti
authors.forename: J
authors.initials: J
authors.lastname: Kozempel
authors.forename: U
authors.initials: U
authors.lastname: Holzwarth
authors.forename: F
authors.initials: F
authors.lastname: Franchini
authors.forename: F
authors.initials: F
authors.lastname: Rossi
mesh_terms: D000818:Animals; D041702:BALB 3T3 Cells / Q000000981:diagnostic imaging* / Q000378:metabolism*; D002460:Cell Line; D002563:Cerium / Q000032:analysis* / Q000737:chemistry / Q000493:pharmacokinetics*; D002565:Cerium Radioisotopes / Q000032:analysis* / Q000737:chemistry / Q000493:pharmacokinetics*; D017357:Cyclotrons; D005720:Gamma Rays; D051379:Mice; D053758:Nanoparticles / Q000032:analysis; D011877:Radionuclide Imaging
chemical_list: D002565:Cerium Radioisotopes; D002563:Cerium; C030583:ceric oxide