title: Genetic expression of an amyloid peptide fragment and analysis of formylated products.
abstract: The model amyloid peptide AAKLVFF was expressed as a His-tagged fusion protein with the immunoglobulin-binding domain B1 of streptococcal protein G (GB1), a small (56 residues), stable, single-domain protein. It is shown that expression of this model amyloid peptide is possible and is not hindered by aggregation. Formylation side reactions during the CNBr cleavage are investigated via synthesis of selectively formylated peptides.
authors.affiliation: School of Chemistry, Food Science and Pharmacy, University of Reading, Whiteknights, Reading, UK.
authors.forename: G
authors.initials: G
authors.lastname: Cheng
authors.forename: C
authors.initials: C
authors.lastname: Krasel
authors.forename: H G
authors.initials: HG
authors.lastname: Zhou
authors.forename: D
authors.initials: D
authors.lastname: Chappell
authors.forename: I W
authors.initials: IW
authors.lastname: Hamley
mesh_terms: D000595:Amino Acid Sequence; D016229:Amyloid beta-Peptides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism; D057930:Click Chemistry; D005563:Formic Acid Esters / Q000737:chemistry*; D005818:Genetic Engineering / Q000379:methods; D006639:Histidine / Q000737:chemistry; D008958:Models, Molecular; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: C559205:AAKLVFF; D016229:Amyloid beta-Peptides; D001426:Bacterial Proteins; D005563:Formic Acid Esters; C034284:IgG Fc-binding protein, Streptococcus; D010446:Peptide Fragments; D006639:Histidine