title: In vitro antioxidant and anti-inflammatory activities of protocatechualdehyde isolated from Phellinus gilvus.
abstract: Although various biological activities of Phellinus gilvus (PG) have been reported, the active compounds responsible for these effects are not known. Here, we evaluated the activity of various solvent extracts of PG, and found the ethyl acetate extract (Fd) to be the most active fraction, showing a strong DPPH free radical scavenging activity, and inhibitory effects on LPS-induced nitric oxide (NO) production and COX-2 mRNA expression in RAW264.7 macrophages. Six major compounds were identified from the ethyl acetate extract of PG, and protocatechualdehyde (PCA) was supposed to be the major phenolic compound of PG responsible for its DPPH free radical scavenging activity and its inhibitory effects on LPS-induced NO production in RAW264.7 cells. Further in vitro and in vivo experiments are currently underway to confirm this observation and to investigate the detailed molecular mechanisms involved in the process as well as the biological activities of other fractions of Fd.
authors.affiliation: College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea.
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Chang
authors.forename: Elias
authors.initials: E
authors.lastname: Gebru
authors.forename: Sam-Pin
authors.initials: SP
authors.lastname: Lee
authors.forename: Man-Hee
authors.initials: MH
authors.lastname: Rhee
authors.forename: Jong-Choon
authors.initials: JC
authors.lastname: Kim
authors.forename: Henrique
authors.initials: H
authors.lastname: Cheng
authors.forename: Seung-Chun
authors.initials: SC
authors.lastname: Park
mesh_terms: D000085:Acetates / Q000302:isolation & purification / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D001487:Basidiomycota / Q000737:chemistry*; D001547:Benzaldehydes / Q000302:isolation & purification* / Q000494:pharmacology*; D002396:Catechols / Q000302:isolation & purification* / Q000494:pharmacology*; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D051379:Mice; D008969:Molecular Sequence Data; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D000085:Acetates; D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D001547:Benzaldehydes; D002396:Catechols; D012333:RNA, Messenger; D009569:Nitric Oxide; C005581:protocatechualdehyde; C007650:ethyl acetate; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2