title: Direct pulp capping effect with experimentally developed adhesive resin systems containing reparative dentin-promoting agents on rat pulp: mixed amounts of additives and their effect on wound healing.
abstract: This study examined the wound-healing process of exposed rat pulp when treated with experimental adhesive resin systems. The experimental direct pulp capping adhesive resin systems were composed of primer-I, primer-II and an experimental bonding agent. Primer-I was Clearfil SE Bond (CSE) primer containing 1.0 or 5.0 wt% CaCl(2), and primer-II was CSE primer containing 0.1, 1.0 or 5.0 wt% compound of equal mole of pA and pB with synthetic peptides derived from dentin matrix protein 1 (DMP1). Primer-I containing 1.0 and 5.0 wt% CaCl(2) was assigned to the experimental groups 1-3 and 4-6, respectively. Primer-II containing 0.1, 1.0 or 5.0 wt% compound of pA and pB was assigned to the experimental groups 1 and 4, 2 and 5, and 3 and 6, respectively. In all experimental groups, CSE bond containing 10 wt% hydroxyapatite powder was used as the experimental bonding agent. The positive control teeth were capped with calcium hydroxide preparation (Dycal), and the negative control teeth were capped with CSE. The specimens were alternately stained with Mayer's H&E and the enhanced polymer one-step staining methods. Experimental groups 1, 4, 5 and 6 showed a higher level of reparative dentin formation compared to the negative control 14 days postoperatively. At 28 days postoperatively, all experimental groups showed the formation of extensive reparative dentin, and experimental groups 4, 5 and 6 demonstrated similar dentin bridge formation as that of the positive control. How quickly reparative dentin formation occurs may depend on the concentration of CaCl(2) and the compound of pA and pB in the experimental primer.
authors.affiliation: Advanced Operative Dentistry-Endodontics, The Nippon Dental University Graduate School of Life Dentistry at Niigata, Niigata, Japan. taira.y@ngt.ndu.ac.jp
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Taira
authors.forename: Koichi
authors.initials: K
authors.lastname: Shinkai
authors.forename: Masaya
authors.initials: M
authors.lastname: Suzuki
authors.forename: Chikage
authors.initials: C
authors.lastname: Kato
authors.forename: Yoshiroh
authors.initials: Y
authors.lastname: Katoh
mesh_terms: D000818:Animals; D002122:Calcium Chloride / Q000627:therapeutic use; D002126:Calcium Hydroxide / Q000627:therapeutic use; D001840:Dental Bonding; D003782:Dental Pulp / Q000187:drug effects* / Q000473:pathology; D003785:Dental Pulp Capping / Q000379:methods*; D003789:Dental Pulp Exposure / Q000628:therapy; D003790:Dental Pulp Necrosis / Q000473:pathology; D003793:Dental Restoration, Permanent; D003809:Dentin, Secondary / Q000187:drug effects* / Q000473:pathology; D017886:Durapatite / Q000627:therapeutic use; D016326:Extracellular Matrix Proteins / Q000627:therapeutic use*; D008297:Male; D008689:Methacrylates / Q000737:chemistry; D008903:Minerals / Q000627:therapeutic use; D009804:Odontoblasts / Q000473:pathology; D010446:Peptide Fragments / Q000627:therapeutic use*; D010750:Phosphoproteins / Q000627:therapeutic use*; D055668:Photochemical Processes; D058105:Polymerization; D057872:Pulp Capping and Pulpectomy Agents / Q000627:therapeutic use*; D011671:Pulpitis / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D019279:Resin Cements / Q000737:chemistry*; D013997:Time Factors; D014945:Wound Healing / Q000187:drug effects
chemical_list: C112828:Clearfil AP-X; C438313:Clearfil SE Bond; C081635:Dmp1 protein, rat; D016326:Extracellular Matrix Proteins; D008689:Methacrylates; D008903:Minerals; D010446:Peptide Fragments; D010750:Phosphoproteins; D057872:Pulp Capping and Pulpectomy Agents; D019279:Resin Cements; C025702:Dycal; D017886:Durapatite; D002122:Calcium Chloride; D002126:Calcium Hydroxide