title: Evaluation of pH-sensitive poly(β-amino ester)-graft-poly(ethylene glycol) and its usefulness as a pH-sensor and protein carrier.
abstract: In this study, some possible biomedical applications of a pH-sensitive and amphiphilic copolymer as a pH sensor and protein delivery system are reported. PAE-g-PEG was used as a pH-sensitive polymer that can exhibit a sharp pH-dependent transition. Various fluorescent dyes including pyrene and RITC can be used to label the pH-sensitive polymer PAE-g-PEG, which was evaluated for protein encapsulation. pH-sensing was possible by observing excimer formation of the labeled pyrene via pH-dependent expansion of the polymeric chain. Also, it was confirmed that FITC-BSA could be entrapped in RITC-labeled pH-sensitive micelles of PAE-g-PEG by FRET. As a result, PAE-g-PEG can be a pH sensor and carrier for protein delivery.
authors.affiliation: Department of Polymer Science and Engineering, Sungkyunkwan University, Suwon, Gyeonggi 440-746, Korea.
authors.forename: Min Sang
authors.initials: MS
authors.lastname: Kim
authors.forename: Guang Hui
authors.initials: GH
authors.lastname: Gao
authors.forename: Seong Woo
authors.initials: SW
authors.lastname: Kang
authors.forename: Doo Sung
authors.initials: DS
authors.lastname: Lee
mesh_terms: D000818:Animals; D002412:Cations / Q000737:chemistry; D002417:Cattle; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D021381:Protein Transport; D012710:Serum Albumin, Bovine / Q000737:chemistry*
chemical_list: D002412:Cations; D011108:Polymers; C039217:fluorescein isothiocyanate bovine serum albumin; C507253:poly(beta-amino ester); D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; D016650:Fluorescein-5-isothiocyanate