title: Biocatalytic generation of Mn(III)-chelate as a chemical oxidant of different environmental contaminants.
abstract: The objective of this study was to investigate the enzymatic generation of the Mn(3+) -malonate complex and its application to the process of oxidizing several organic compounds. The experimental set-up consisted of an enzymatic reactor coupled to an ultrafiltration membrane, providing continuous generation of Mn(3+) -malonate from a reaction medium containing versatile peroxidase (an enzyme produced by Bjerkandera adusta strain BOS55), H(2) O(2) , MnSO(4,) and malonate. The effluent of the enzymatic reactor was introduced into a batch-stirred reactor to oxidize three different classes of compounds: an azo dye (Orange II), three natural and synthetic estrogens, and a polycyclic aromatic hydrocarbon (anthracene). The enzymatic reactor provided the Mn(3+) complex under steady-state conditions, and this oxidative species was able to transform the three classes of xenobiotics considerably (90-99%) with negligible loss of activity.
authors.affiliation: Dept. of Chemical Engineering, ETSE, Lope Gómez de Marzoa, Santiago de Compostela 15782, Spain. roberto.taboada@rai.usc.es
authors.forename: Roberto
authors.initials: R
authors.lastname: Taboada-Puig
authors.forename: Thelmo
authors.initials: T
authors.lastname: Lú-Chau
authors.forename: Gemma
authors.initials: G
authors.lastname: Eibes
authors.forename: María Teresa
authors.initials: MT
authors.lastname: Moreira
authors.forename: Gumersindo
authors.initials: G
authors.lastname: Feijoo
authors.forename: Juan Manuel
authors.initials: JM
authors.lastname: Lema
mesh_terms: D000873:Anthracenes; D001391:Azo Compounds; D001557:Benzenesulfonates; D055162:Biocatalysis; D002614:Chelating Agents; D004785:Environmental Pollutants / Q000737:chemistry*; D052918:Environmental Restoration and Remediation / Q000379:methods*; D004867:Equipment Design; D004967:Estrogens; D008314:Malonates / Q000737:chemistry*; D008345:Manganese; D016877:Oxidants / Q000737:chemistry*; D009195:Peroxidase; D011083:Polycyclic Compounds
chemical_list: D000873:Anthracenes; D001391:Azo Compounds; D001557:Benzenesulfonates; D002614:Chelating Agents; D004785:Environmental Pollutants; D004967:Estrogens; D008314:Malonates; D016877:Oxidants; D011083:Polycyclic Compounds; D008345:Manganese; D009195:Peroxidase; C034020:anthracene; C014722:2-naphthol orange