title: [Cytotoxic effect of IL-2/IL-15 stimulated cord blood derived NK cells on K562/Jurkat cell lines].
abstract: The aim of this study was to explore the cytotoxicity of fresh cord blood(CB) NK cells and the influence of IL-12 and IL-15 on activity of the NK cells killing K562 and Jurkat cells lines. The NK cells were isolated from cord blood by depleting CD3(+) cells and then enriching CD56(+) cells using sorting with immunomagnetic beads. The experiment was divided into 3 groups: group A (fresh CB-NK cells without cytokines), group B (CB-NK cells cultured by IL-2) and group C (CB-NK cells cultured by IL-2 and IL-15). The purity of NK cells was determined by flow cytometry; the cytotoxity of fresh and different cytokine-treated CB-NK cells on K562 and Jurkat cell lines was detected by LDH release test. The results showed that the purity of NK cells before and after sorting was 14.88 ± 9.2% and 92.39 ± 0.8% respectively. After culture for 3 days, NK-forming colony amounts in group B and group C were 148.60 ± 13.0 and 831.80 ± 23.0 respectively, the comparison between group B and group C showed the significant difference (p < 0.05). The cytotoxicities of NK cells in group A, B and C on K562 and Jurkat cell lines were 27.76 ± 8.8%, 61.90 ± 9.1% and 87.62 ± 3.7%; 29.32 ± 2.5%, 69.43 ± 4.4% and 92.95 ± 3.2% respectively, the difference was significant (p < 0.05). It is concluded that the fresh isolated CB-NK cells show low cytotoxic activity. After stimulated with IL-2 or IL-2 plus IL15, cytotoxicity of CB-NK cells increases obviously, the effect of IL-2 plus IL-15 is much better than IL-2 alone for promoting the growth and enhancing the cytotoxicity of CB-NK cells.
authors.affiliation: Department of Pediatrics, SUN Yat-Sen Memorial Hospital, Guangzhou 510120, Guangdong Province, China.
authors.forename: Bi-Hong
authors.initials: BH
authors.lastname: Zhang
authors.forename: Yan-Feng
authors.initials: YF
authors.lastname: Wu
authors.forename: Dan-Yang
authors.initials: DY
authors.lastname: Cen
authors.forename: Jing
authors.initials: J
authors.lastname: Wei
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.forename: Chun
authors.initials: C
authors.lastname: Chen
mesh_terms: D003602:Cytotoxicity, Immunologic / Q000187:drug effects / Q000276:immunology*; D005312:Fetal Blood / Q000187:drug effects / Q000276:immunology; D006801:Humans; D019409:Interleukin-15 / Q000494:pharmacology*; D007376:Interleukin-2 / Q000494:pharmacology*; D019169:Jurkat Cells; D020014:K562 Cells; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology*
chemical_list: D019409:Interleukin-15; D007376:Interleukin-2