title: Human serum albumin binding and cytotoxicity studies of surfactant-cobalt(III) complex containing 1,10-phenanthroline ligand.
abstract: The characteristics of the binding reaction of surfactant-cobalt(III) complex, cis-[Co(phen)₂(C₁₄H₂₉NH₂)]Cl₂·3H₂O (phen=1,10-phenanthroline, C₁₄H₂₉NH₂=tetradecylamine) with human serum albumin (HSA) were studied by fluorescence and UV-vis absorption spectroscopy. In addition, the effect of the surfactant-cobalt(III) complex on the conformation of HSA was analysed using synchronous fluorescence spectroscopy. The experimental results showed that surfactant-cobalt(III) complex caused the fluorescence quenching of HSA through a combination of static and dynamic quenching. The number of binding sites (n) and apparent binding constant (K(a)) of surfactant-cobalt(III) complex (above and below the critical micelle concentration (cmc) were determined at various temperatures. According to the thermodynamic parameters, it is likely that hydrophobic interactions are involved in the binding process. The cancer chemotherapeutic potential of surfactant-cobalt(III) complex on ME-180 cervical cancer cell was determined using MTT assay and specific staining techniques. The complex affected the viability of the cells significantly and the cells succumbed through an apoptosis process as seen in the nuclear morphology and cytoplasmic features. In addition, single-cell electrophoresis indicated DNA damage.
authors.affiliation: Medical Photonics Group, ISIC, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland. raj_sen81@yahoo.co.in
authors.forename: R Senthil
authors.initials: RS
authors.lastname: Kumar
authors.forename: Preethy
authors.initials: P
authors.lastname: Paul
authors.forename: A
authors.initials: A
authors.lastname: Riyasdeen
authors.forename: Georges
authors.initials: G
authors.lastname: Wagniéres
authors.forename: Hubert
authors.initials: H
authors.lastname: van den Bergh
authors.forename: M A
authors.initials: MA
authors.lastname: Akbarsha
authors.forename: S
authors.initials: S
authors.lastname: Arunachalam
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000493:pharmacokinetics*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D003035:Cobalt / Q000737:chemistry*; D006801:Humans; D015394:Molecular Structure; D010618:Phenanthrolines / Q000737:chemistry*; D012709:Serum Albumin / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013501:Surface-Active Agents / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D010618:Phenanthrolines; D012709:Serum Albumin; D013501:Surface-Active Agents; D003035:Cobalt; C025205:1,10-phenanthroline