title: Metabolic effects of feeding ethanol or propanol to postpartum transition Holstein cows.
abstract: Eight lactating Holstein cows implanted with a ruminal cannula and permanent indwelling catheters in major splanchnic blood vessels were used to investigate metabolism of propanol and ethanol in the postpartum transition period. Cows were randomly allocated to 1 of 4 treatments in a randomized design with a 2 by 2 factorial arrangement of treatments. Factor 1 was 2.6g of calcium carbonate/kg of dry matter (DM) versus 1.5 g of 2-hydroxy-4-(methylthio)-butanoic acid isopropyl ester/kg of DM. Factor 2 was supplementation with 14 g of propanol/kg of DM (propanol treatment; PT) versus 14 g of ethanol/kg of DM (ethanol treatment; ET). Only factor 2 data are presented in the present paper. Treatments were administered in silage-based total mixed rations and cows were fed the experimental total mixed ration from the day of parturition. Daily rations were fed in 3 equally sized portions at 8-h intervals. Eight hourly sets of ruminal fluid, arterial, and hepatic portal and hepatic vein samples were collected at day -15 ± 5, 4, 15, and 29 relative to parturition. Dry matter intake and milk yield increased with days in milk (DIM), but were not affected by treatment. From prepartum to 4 DIM ruminal concentrations of propanol and ethanol increased with PT and ET, respectively. Postpartum, alcohol intake increased 49% in PT and 34% in ET from 4 to 29 d in milk, respectively. Ruminal concentrations of the alcohols remained unaffected by DIM. Treatments did not affect total ruminal volatile fatty acid concentrations, but the molar proportion of acetate increased in ET and the molar proportion of propionate increased in PT compared with the contrasting treatment. Propanol treatment decreased milk fat content at 15 to 29 DIM compared with ET. The net portal release of propanol and ethanol increased with increasing ruminal concentration of the respective alcohol. The portal release of alcohol accounted for 43 to 85% of ingested propanol and 36 to 57% of ingested ethanol. Hepatic uptake of propanol and ethanol equaled the net portal flux and no effect of treatment was detected for net splanchnic release of propanol and ethanol. In conclusion, ruminal metabolism is a major component of alcohol metabolism in dairy cows. The postpartum transition dairy cow has sufficient metabolic capacity to cope with high dietary concentrations of primary alcohols even when alcohol intake is abruptly increased at the day of calving. Alcohol intake affects milk fat content and alcohol composition of silage might be important to improve predictions of milk composition.
authors.affiliation: Department of Animal Health and Bioscience, Faculty of Agricultural Sciences, Aarhus University, DK-8830 Tjele, Denmark.
authors.forename: B M L
authors.initials: BM
authors.lastname: Raun
authors.forename: N B
authors.initials: NB
authors.lastname: Kristensen
mesh_terms: D000433:1-Propanol / Q000008:administration & dosage / Q000378:metabolism*; D000818:Animals; D002417:Cattle / Q000378:metabolism*; D000431:Ethanol / Q000008:administration & dosage / Q000378:metabolism*; D005260:Female; D005285:Fermentation; D008099:Liver / Q000098:blood supply / Q000378:metabolism; D011168:Portal System / Q000378:metabolism; D049590:Postpartum Period / Q000378:metabolism*; D012417:Rumen / Q000378:metabolism; D012820:Silage; D013045:Species Specificity
chemical_list: D000431:Ethanol; D000433:1-Propanol