title: Synthesis, characterization, and study on HeLa cells activity of a dinuclear complex [Cu4(phen)4(H2O)2]·(pyri)·3H2O.
abstract: The complex [Cu4(phen)4(H2O)2]·(pyri)·3H2O(where phen=1,10-phenanthroline and pyri=3,5-pyridine dicarboxylic acid)has been synthesized and characterized. IR spectra, elemental analysis and X-ray single-crystal diffraction were carried out to determine the composition and crystal structure of the complex. The binding of the complex with HC-DNA (HeLa cells DNA, which was extracted by ourselves) was investigated by fluorescence spectrum. Gel electrophoresis assay demonstrates the ability of the complex to cleave the extracted HC-DNA. Additionally, the complex exhibited a significant cytotoxic specificity and cancer cell inhibitory rate. The apoptotic tests indicate that the complex have an apoptotic effect on HeLa cells.
authors.affiliation: Department of Coordination Chemistry, Shenyang University of Chemical Technology, International Key Laboratory of Shenyang Inorganic Molecule-Based Chemical, Shenyang 110142, China. ejgao@yahoo.com.cn
authors.forename: En-jun
authors.initials: EJ
authors.lastname: Gao
authors.forename: Lin
authors.initials: L
authors.lastname: Lin
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Ren-shu
authors.initials: RS
authors.lastname: Wang
authors.forename: Ming-chang
authors.initials: MC
authors.lastname: Zhu
authors.forename: Shi-hua
authors.initials: SH
authors.lastname: Liu
authors.forename: Tie-dong
authors.initials: TD
authors.lastname: Sun
authors.forename: Wei
authors.initials: W
authors.lastname: Jiao
authors.forename: Vladimirovich-Zadesenets
authors.initials: VZ
authors.lastname: Andrey
mesh_terms: D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D018360:Crystallography, X-Ray; D004247:DNA / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D006367:HeLa Cells; D006801:Humans; D008958:Models, Molecular; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D013237:Stereoisomerism; D013329:Structure-Activity Relationship; D014407:Tumor Cells, Cultured
chemical_list: D000970:Antineoplastic Agents; D056831:Coordination Complexes; D009942:Organometallic Compounds; D004247:DNA