title: Proteomics-based discovery of koranimine, a cyclic imine natural product.
abstract: Nonribosomal peptide synthetases (NRPSs) and polyketide synthases (PKSs) are large enzymes responsible for the biosynthesis of medically and ecologically important secondary metabolites. In a previous report, we described a proteomics approach to screen for expressed NRPSs or PKSs from bacteria with or without sequenced genomes. Here we used this proteome mining approach to discover a novel natural product arising from rare adenylation (A) and reductase (Red) domains in its biosynthetic machinery. We also cloned the entire gene cluster and elucidated the biosynthesis of the new compound, which is produced by an unsequenced Bacillus sp. isolated from soil collected in Koran, Louisiana.
authors.affiliation: Institute of Genomic Biology, University of Illinois Urbana-Champaign, 1206 West Gregory Drive, Urbana, Illinois 61801, USA.
authors.forename: Bradley S
authors.initials: BS
authors.lastname: Evans
authors.forename: Ioanna
authors.initials: I
authors.lastname: Ntai
authors.forename: Yunqiu
authors.initials: Y
authors.lastname: Chen
authors.forename: Sarah J
authors.initials: SJ
authors.lastname: Robinson
authors.forename: Neil L
authors.initials: NL
authors.lastname: Kelleher
mesh_terms: D000595:Amino Acid Sequence; D001688:Biological Products / Q000737:chemistry* / Q000235:genetics; D003500:Cyclization; D007097:Imines / Q000737:chemistry*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010456:Peptides, Cyclic / Q000737:chemistry* / Q000235:genetics; D040901:Proteomics
chemical_list: D001688:Biological Products; D007097:Imines; D010456:Peptides, Cyclic