title: H HRMAS NMR Derived Bio-markers Related to Tumor Grade, Tumor Cell Fraction, and Cell Proliferation in Prostate Tissue Samples.
abstract: A high-resolution magic angle spinning NMR spectroscopic approach is presented for evaluating the occurrence, amount and aggressiveness of cancer in human prostate tissue samples. Using this technique, key metabolites in malignant and non-malignant samples (n = 149) were identified, and patterns of their relative abundance were analyzed by multivariate statistical methods. Ratios of various metabolites - including (glycerophophorylcholine + phosphorylcholine)/creatine, myo-inositol/scyllo-inositol, scyllo-inositol/creatine, choline/creatine, and citrate/creatine - correlated with: i) for non-malignant tissue samples, the distance to the nearest tumor and its Gleason score and; ii) the fraction of tumor cells present in the sample; and iii) tumor cell proliferation (Ki67 labelling index). This NMR-based approach allows the extraction of information that could be useful for developing novel diagnostic methods for prostate cancer.
authors.affiliation: Departments of Radiation Sciences, Diagnostic Radiology.
authors.forename: Katarina
authors.initials: K
authors.lastname: Stenman
authors.forename: Pär
authors.initials: P
authors.lastname: Stattin
authors.forename: Hans
authors.initials: H
authors.lastname: Stenlund
authors.forename: Katrine
authors.initials: K
authors.lastname: Riklund
authors.forename: Gerhard
authors.initials: G
authors.lastname: Gröbner
authors.forename: Anders
authors.initials: A
authors.lastname: Bergh