title: Improved BC method of Compomat G4 for expression of BCs twice from whole blood in top and top bags.
abstract: The aim of this paper was to evaluate an improved buffy coat (BC) method of Compomat G4 for automated expression of BCs twice from whole blood (WB) in top and top (T&T) bags. WB was separated using hard spin centrifugation (2,988g, 10 min) into layers of blood components by specific gravity, and different components were subsequently expressed into satellite bags in the T&T system using the manual BC method, the conventional BC method of G4, and our improved BC method of G4. In the improved BC method, an accessorial device we have named a 'gravity press' was designed and installed on the top flat of G4 to produce gravitational pressure on the plasma bag so as to exclude air and some of plasma to the upper compartment of the slide after BCs were expressed for the first time. The residual BCs in the upper compartment were expressed a second time by extending the upper press once more. All of the pooled BCs were centrifuged by soft spin (402g, 10 min) and upper platelet-rich supernatant was manually expressed into a platelet container by the plasma extractor. In vitro studies of blood components and pooled platelet concentrates (PCs) revealed no significant differences in BC blood components and platelet recovery of pooled platelets (61 ± 9 vs. 60 ± 7%, n = 12, p > 0.05) between the improved BC method and the conventional BC method; all components met our specifications for blood products. We suggest that the new BC method for use of T&T bags may improve the collection of BCs.
authors.affiliation: Hematology Laboratory, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510260, China.
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Deng
authors.affiliation: Hematology Laboratory, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510260, China.
authors.forename: Xiao-Man
authors.initials: XM
authors.lastname: Wu
authors.affiliation: Institution of Blood Transfusion, Guangzhou Blood Center, Guangzhou, 510095, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Institution of Blood Transfusion, Guangzhou Blood Center, Guangzhou, 510095, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Luo
authors.affiliation: Hematology Laboratory, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510260, China.
authors.forename: Hong-Yun
authors.initials: HY
authors.lastname: Jia
authors.affiliation: Hematology Laboratory, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510260, China.
authors.forename: Zhong-Ying
authors.initials: ZY
authors.lastname: Wang
authors.affiliation: Institution of Blood Transfusion, Guangzhou Blood Center, Guangzhou, 510095, China.
authors.forename: Bo
authors.initials: B
authors.lastname: He
authors.affiliation: Institution of Blood Transfusion, Guangzhou Blood Center, Guangzhou, 510095, China. callmm@126.com.
authors.forename: Chuan-Xi
authors.initials: CX
authors.lastname: Wang
mesh_terms: D058621:Blood Buffy Coat / Q000166:cytology*; D001781:Blood Component Removal / Q000295:instrumentation / Q000379:methods*; D001792:Blood Platelets / Q000166:cytology*; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D002498:Centrifugation / Q000379:methods; D018470:Gravity, Altered; D006801:Humans; D007962:Leukocytes / Q000166:cytology; D010976:Platelet Count