title: Real-time PCR investigation on the expression of sboA and ituD genes in Bacillus spp.
abstract: OBJECTIVE
  To investigate the expression of sboA and ituD genes among strains of Bacillus spp. at different pH and temperature.
  
  
  RESULTS
  Different Bacillus strains from the Amazon basin and Bacillus subtilis ATCC 19659 were investigated for the production of subtilosin A and iturin A by qRT-PCR, analysing sboA and ituD gene expression under different culture conditions. Amazonian strains presented a general gene expression level lower than B. subtilis ATCC 19659 for sboA. In contrast, when analysing the expression of ituD gene, the strains from the Amazon, particularly P40 and P45B, exhibited higher levels of expression. Changes in pH (6 and 8) and temperature (37 and 42 °C) caused a decrease in sboA expression, but increased ituD expression among strains from Amazonian environment.
  
  
  CONCLUSIONS
  Temperature and pH have an important influence on the expression of genes sboA (subtilosin A) and ituD (iturin A) among Bacillus spp. The strains P40 and P45B can be useful for the production of antimicrobial peptide iturin A.
  
  
  CONCLUSIONS
  Monitoring the expression of essential biosynthetic genes by qRT-PCR is a valuable tool for optimization of the production of antimicrobial peptides.
authors.affiliation: Laboratório de Bioquímica e Microbiologia Aplicada, Departamento de Ciência de Alimentos, Universidade Federal do Rio Grande do Sul, 91501-970 Porto Alegre, Brasil.
authors.forename: R V
authors.initials: RV
authors.lastname: Velho
authors.forename: D G G
authors.initials: DG
authors.lastname: Caldas
authors.forename: L F C
authors.initials: LF
authors.lastname: Medina
authors.forename: S M
authors.initials: SM
authors.lastname: Tsai
authors.forename: A
authors.initials: A
authors.lastname: Brandelli
mesh_terms: D001407:Bacillus / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D001412:Bacillus subtilis / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D001430:Bacteriocins / Q000235:genetics*; D001938:Brazil; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D010456:Peptides, Cyclic / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D001430:Bacteriocins; D010456:Peptides, Cyclic; C013579:iturin A; C047828:sboA protein, Bacillus subtilis