title: Profiling of a few immune responsive genes expressed in postlarvae of Fenneropenaeus indicus challenged with Vibrio harveyi D3.
abstract: We identified 38 genes and eight hypothetical proteins by sequencing of 1200 clones from a Vibrio harveyi challenged Fenneropenaeus indicus subtracted cDNA library. Based on physiological roles and functions these genes were categorized into 10 groups with ∼29% of the sequences having no matches in the databases. Immune related transcripts in the library were carboxypeptidase B, ferritin, translationally controlled tumor proteins (TCTP), hemocyanin, chitinase and serine carboxy peptidase. Remarkably, qPCR results imply 4.15, 3.45 and 1.86-fold rises in expression of ferritin, TCTP and hemocyanin transcripts respectively. Additionally, minor upregulation of other immune relevant genes lectin, penaeidin, crustin, MnSOD was observed in the challenged postlarvae.
authors.affiliation: Biological Oceanography Division, National Institute of Oceanography, Council of Scientific and Industrial Research, Dona Paula, Goa 403 004, India.
authors.forename: S
authors.initials: S
authors.lastname: Nayak
authors.forename: K M
authors.initials: KM
authors.lastname: Ajay
authors.forename: N
authors.initials: N
authors.lastname: Ramaiah
authors.forename: Ram M
authors.initials: RM
authors.lastname: Meena
authors.forename: R A
authors.initials: RA
authors.lastname: Sreepada
mesh_terms: D000818:Animals; D017756:Aquaculture; D004266:DNA Virus Infections / Q000276:immunology*; D015870:Gene Expression; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D007814:Larva / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D033561:Penaeidae / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D014733:Vibrio / Q000276:immunology*; D014735:Vibrio Infections / Q000276:immunology*