title: Impact of rpoB mutations on reduced vancomycin susceptibility in Staphylococcus aureus.
abstract: Of 38 vancomycin-intermediate Staphylococcus aureus (VISA) clinical strains, 27 (71%) possessed a mutation(s) in rpoB encoding the β-subunit of RNA polymerase. Furthermore, 95.6% of the rifampin-resistant mutants obtained from 9 methicillin-resistant S. aureus (MRSA) clinical isolates showed decreased vancomycin susceptibilities. These data indicate the involvement of an rpoB mutation in VISA phenotype expression.
authors.affiliation: Department of Bacteriology, Graduate School of Medicine, Juntendo University, 2-1-1 Hongo, Bunkyo-ku, Tokyo, Japan.
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Watanabe
authors.forename: Longzhu
authors.initials: L
authors.lastname: Cui
authors.forename: Yuki
authors.initials: Y
authors.lastname: Katayama
authors.forename: Kishii
authors.initials: K
authors.lastname: Kozue
authors.forename: Keiichi
authors.initials: K
authors.lastname: Hiramatsu
mesh_terms: D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D006801:Humans; D008826:Microbial Sensitivity Tests; D020125:Mutation, Missense; D013203:Staphylococcal Infections / Q000382:microbiology; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D014640:Vancomycin / Q000494:pharmacology*; D020713:Vancomycin Resistance
chemical_list: D014640:Vancomycin; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit