title: Use of nested polymerase chain reaction based on sequence-based typing of clinical samples to determine the source of infection for hospital-acquired Legionnaires' disease.
abstract: The source of infection of a hospital-acquired Legionnaires' disease case was determined for the first time by nested polymerase chain reaction based on sequence-based typing. The typing was performed directly on DNA extracted from tissue samples, allowing a rapid epidemiological correlation with environmental isolates.
authors.affiliation: Department of Infectious, Parasitic, and Immune-Mediated Diseases, Istituto Superiore di Sanità, Rome, Italy. maria.scaturro@iss.it
authors.forename: Maria
authors.initials: M
authors.lastname: Scaturro
authors.forename: Stefano
authors.initials: S
authors.lastname: Fontana
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Ricci
mesh_terms: D003428:Cross Infection / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis; D017809:Fatal Outcome; D005260:Female; D006801:Humans; D016952:Legionella pneumophila / Q000235:genetics* / Q000302:isolation & purification; D007877:Legionnaires' Disease / Q000382:microbiology* / Q000635:transmission; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D004269:DNA, Bacterial