title: [Role of HLA protein three-dimensional conformation difference in unrelated hematopoietic stem cell transplantation].
abstract: This study was purposed to investigate the value of Histocheck and HLA-Matchmaker softwares in evaluating influence of HLA protein three dimensional conformation among individuals on outcome of unrelated donor hematopoietic stem cell transplantation (URHSCT). Data of the HLA-A/B/C/DRB1/DQB1 genotypes from 62 cases of URHSCT (HLA-allele 10/10 match 30 cases, 9/10 match 32 cases) were input into Histocheck and HLA-Matchmaker softwares respectively. The relationship between the software dissimilar scores and the 1 year overall survival (OS), incidence of aGVHD of III-IV grade and relapse rate was analyzed. The results showed that (1) with increase of the Histocheck scores, incidence of aGVHD of III-IV increased from 0% to 20% (p = 0.25), while no or mild aGVHD occurred in 70% cases with the high scores. For the relapsed cases, there was no significant difference between the cases with low scores and with highest scores (relapse rate 20%) except that 9 cases had no relapse in the group with higher score (11 - 20). (2) the analysis using HLA matchmaker software showed that incidence of aGVHD of III-IV grade increased with the increase of numbers of mismatch Eplets, arranging from 0% to 30%, the incidence of moderate aGVHD reduced (p = 0.019), whereas 60% cases in highest scores group had moderate aGVHD. No relapse occurred in the group with higher scores (≥ 3) (n = 10), whereas high relapse rate appeared in the lower score group (20%, p = 0.54). It is concluded that the value of Histocheck and HLA-Mtchmaker software for analysing the outcome of URHSCT may be similar despite of different calculating methods; for the certain pair of recipient and donor, correlation of the two score systems with incidence of aGVHD and relapse rate is similar, but with less accuracy; The HLA Matchmaker software appears better than Histocheck software in terms of correlation.
authors.affiliation: Beijing Daopei Hospital, Beijing 100049, China. zhangxian@ludaopei.org
authors.forename: Xian
authors.initials: X
authors.lastname: Zhang
authors.forename: Yan-Ling
authors.initials: YL
authors.lastname: Zhang
authors.forename: Jian-Ling
authors.initials: JL
authors.lastname: Wang
authors.forename: Chun-Rong
authors.initials: CR
authors.lastname: Tong
authors.forename: Peng
authors.initials: P
authors.lastname: Cai
authors.forename: Hong-Xing
authors.initials: HX
authors.lastname: Liu
authors.forename: Jing-Bo
authors.initials: JB
authors.lastname: Wang
authors.forename: Xing-Yu
authors.initials: XY
authors.lastname: Cao
authors.forename: Yu-Ming
authors.initials: YM
authors.lastname: Yin
authors.forename: Yan-Li
authors.initials: YL
authors.lastname: Zhao
authors.forename: Yue
authors.initials: Y
authors.lastname: Lu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Sun
authors.forename: Jia-Rui
authors.initials: JR
authors.lastname: Zhou
authors.forename: Yan-Qu
authors.initials: YQ
authors.lastname: Gao
authors.forename: Tong
authors.initials: T
authors.lastname: Wu
mesh_terms: D000293:Adolescent; D000328:Adult; D000483:Alleles; D002648:Child; D002675:Child, Preschool; D005260:Female; D005838:Genotype; D006086:Graft vs Host Disease; D006680:HLA Antigens / Q000235:genetics*; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006650:Histocompatibility Testing; D006801:Humans; D008297:Male; D011487:Protein Conformation; D012008:Recurrence; D012984:Software; D014019:Tissue Donors; D055815:Young Adult
chemical_list: D006680:HLA Antigens