title: Kinetic spectrophotometric methods for the determination of artificial sweetener (sucralose) in tablets.
abstract: Two simple and sensitive kinetic spectrophotometric methods for the determination of sucralose are described. The first method is based upon a kinetic investigation of the oxidation reaction of the drug with alkaline potassium permanganate at room temperature for a fixed time of 30 min. The absorbance of the green coloured manganate ions produced was measured at 610 nm. The second method is based on the reaction of sucralose with cerium (IV) ammonium sulfate in the presence of perchloric acid with the subsequent measurement of the excess unreacted cerium (IV) ammonium sulfate at 320 nm at a fixed time of 30 min in a thermostated water bath at 60 ± 1 °C. This principle is adopted to develop a kinetic method for sucralose determination. The absorbance concentration plots in both methods were rectilinear over the range 4-16 and 10-30 µg ml(-1) , for the first and second methods, respectively. The different experimental parameters affecting the development and stability of the colours were carefully studied and optimized. The determination of sucralose by rate constant method, fixed concentration method, and fixed-time method was also feasible with calibration equations obtained but the latter method was found to be more applicable. The two methods have been applied successfully to commercial tablets.
authors.affiliation: Faculty of Pharmacy, Department of Pharmaceutical Analytical Chemistry, University of Alexandria, El-Messalah, Alexandria 21521, Egypt. rmmy1973@yahoo.com
authors.forename: Rasha M
authors.initials: RM
authors.lastname: Youssef
authors.forename: Mohamed A
authors.initials: MA
authors.lastname: Korany
authors.forename: Essam F
authors.initials: EF
authors.lastname: Khamis
authors.forename: Hoda
authors.initials: H
authors.lastname: Mahgoub
authors.forename: Miranda F
authors.initials: MF
authors.lastname: Kamal
mesh_terms: D007700:Kinetics; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet / Q000379:methods / Q000592:standards; D013395:Sucrose / Q000031:analogs & derivatives* / Q000032:analysis / Q000493:pharmacokinetics; D013549:Sweetening Agents / Q000032:analysis* / Q000493:pharmacokinetics*; D013607:Tablets
chemical_list: D013549:Sweetening Agents; D013607:Tablets; D013395:Sucrose; C026285:trichlorosucrose