title: CD10 and ICOS expression by multiparametric flow cytometry in angioimmunoblastic T-cell lymphoma.
abstract: Angioimmunoblastic T-cell lymphoma is immunologically defined by the expression of CD10 and the follicular helper T cell (T(FH)) markers such as CXCL13, programmed death-1 (PD-1) and inducible T-cell costimulator (ICOS). This T(FH) profile has been mainly reported by immunohistochemistry. Here, using multiparametric flow cytometry, the relevance of ICOS and PD-1 to angioimmunoblastic T-cell lymphoma diagnosis was evaluated in lymph node (n=15) as well as in peripheral blood (n=13) among a series of 28 angioimmunoblastic T-cell lymphoma cases, in addition to the CD10 expression (available in 26 lymph node and 15 peripheral blood specimens). In this series, CD10 expression was present in 23/26 (88%) lymph node and in 12/15 (80%) peripheral blood cases and ICOS in 13/15 (87%) lymph node and in 6/13 (47%) peripheral blood cases, whereas neither significant CD10 nor ICOS T cells were identified in the control group (lymph nodes with reactive hyperplasia=10, peripheral blood of healthy donors=15). PD-1 expression was less informative as observed in both angioimmunoblastic T-cell lymphoma and control cases. The multiparametric approach allowed us to confirm the frequent blood dissemination in angioimmunoblastic T-cell lymphoma and to show that circulating neoplastic T cells correspond more often to a CD10-positive subset than to an ICOS-positive subset. Consequently, if ICOS constitutes an additional feature for the diagnosis of angioimmunoblastic T-cell lymphoma, it appears less sensitive than CD10 expression for the detection of circulating neoplastic T cells.
authors.affiliation: Laboratoire d'Hématologie Cellulaire, Centre Hospitalier Lyon-Sud, Pierre-Bénite, France.
authors.forename: Lucile
authors.initials: L
authors.lastname: Baseggio
authors.forename: Alexandra
authors.initials: A
authors.lastname: Traverse-Glehen
authors.forename: Françoise
authors.initials: F
authors.lastname: Berger
authors.forename: Martine
authors.initials: M
authors.lastname: Ffrench
authors.forename: Laurent
authors.initials: L
authors.lastname: Jallades
authors.forename: Dominique
authors.initials: D
authors.lastname: Morel
authors.forename: Ghislaine
authors.initials: G
authors.lastname: Goedert
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Magaud
authors.forename: Gilles
authors.initials: G
authors.lastname: Salles
authors.forename: Pascale
authors.initials: P
authors.lastname: Felman
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000032:analysis / Q000096:biosynthesis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis / Q000096:biosynthesis*; D051017:Apoptosis Regulatory Proteins / Q000032:analysis / Q000096:biosynthesis; D014408:Biomarkers, Tumor / Q000032:analysis / Q000097:blood; D002469:Cell Separation; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007119:Immunoblastic Lymphadenopathy / Q000378:metabolism*; D060889:Inducible T-Cell Co-Stimulator Protein; D008198:Lymph Nodes / Q000378:metabolism / Q000473:pathology; D016399:Lymphoma, T-Cell / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009360:Neoplastic Cells, Circulating / Q000378:metabolism*; D015260:Neprilysin / Q000032:analysis / Q000096:biosynthesis*; D061026:Programmed Cell Death 1 Receptor
chemical_list: D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D051017:Apoptosis Regulatory Proteins; D014408:Biomarkers, Tumor; C556692:ICOS protein, human; D060889:Inducible T-Cell Co-Stimulator Protein; C105992:PDCD1 protein, human; D061026:Programmed Cell Death 1 Receptor; D015260:Neprilysin