title: [Effects of oleanolic acid on apoptosis and PTEN expression of Jurkat cells].
abstract: This study was aimed to explore the effects of oleanolic acid on PTEN expression and apoptosis of Jurkat cells. The inhibitory rate was measured by Cell Counting Kit-8. The apoptotic nucleus morphous was observed by Hoechst 33258 staining. The apoptosis rate of Jurkat cells were determined by flow cytometry with Annexin V/PI double staining. PTEN mRNA and protein were detected by quantitative real-time PCR and Western blot respectively. The results showed that oleanolic acid inhibited the proliferation of Jurkat cells in time- and dose-dependent manners. The 50% growth inhibition (IC(50)) at 12, 24 and 48 hours were about 85.35 µmol/L, 53.66 µmol/L and 33.18 µmol/L respectively. Flow cytometric assay showed that the apoptotic rates of Jurkat cells treated with oleanolic acid (0, 40, 80 and 160 µmol/L) for 24 hours were 6.72%, 19.8%, 28.72% and 30.12% (p < 0.05). PTEN mRNA and protein expressions were up-regulated in Jurkat cells treated with oleanolic acid of concentration 80 µmol/L and 160 µmol/L for 24 hours. It is concluded that up-regulation of PTEN mRNA and PTEN protein may be involved in oleanolic acid-induced Jurkat cell apoptosis.
authors.affiliation: Diagnostic and Therapeutic Center of Hematologic Diseases, China Medical University, Shenyang, Liaoning Province, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.forename: Ai-Jun
authors.initials: AJ
authors.lastname: Liao
authors.forename: Bin
authors.initials: B
authors.lastname: Wu
authors.forename: Meng-Yao
authors.initials: MY
authors.lastname: Pan
authors.forename: Zhuo-Gang
authors.initials: ZG
authors.lastname: Liu
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation; D006801:Humans; D019169:Jurkat Cells; D009828:Oleanolic Acid / Q000494:pharmacology*; D051059:PTEN Phosphohydrolase / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D009828:Oleanolic Acid; D051059:PTEN Phosphohydrolase