title: Establishment of an arabinose-inducible system in Stenotrophomonas maltophilia.
abstract: A pBBad22T-derived conditioned arabinose (Ara)-inducible expression system was evaluated in Stenotrophomonas maltophilia (an opportunistic pathogen and has gained increasing attention as a cause of healthcare-associated infection). S. maltophilia cannot grow well when Ara is the sole available carbon source. The induction kinetic study, optimal inducer concentration determination, and depletion experiment were performed by using a xylE gene fusion construct, pBxylE, to monitor the expression of pBBad22T in S. maltophilia. For induction survey, the expression of catechol 2,3-dioxygenase (C23O), encoded by xylE gene, continuously increases during an 8-h induced course and can be modulated by different inducer concentrations. The applied induction condition of pBBad22T in S. maltophilia is the inducer concentration ranging from 0.1% to 0.5% for an induction time of 4 h. For repression evaluation, the C23O expression is rapidly turned off within 30 min after the removal of Ara. Accordingly, the established Ara-inducible system can provide a convenient tool for the study of S. maltophilia.
authors.affiliation: Graduate Institute of Microbiology and Public Health, National Chung-Hsing University, Taichung, 402, Taiwan.
authors.forename: Yi-Wei
authors.initials: YW
authors.lastname: Huang
authors.forename: Rouh-Mei
authors.initials: RM
authors.lastname: Hu
authors.forename: Yu-Ting
authors.initials: YT
authors.lastname: Chiang
authors.forename: Tsao-Chuen
authors.initials: TC
authors.lastname: Chung
authors.forename: Tung-Ching
authors.initials: TC
authors.lastname: Chung
authors.forename: Tsuey-Ching
authors.initials: TC
authors.lastname: Yang
mesh_terms: D001089:Arabinose / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D050557:Catechol 2,3-Dioxygenase / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D020615:Stenotrophomonas maltophilia / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D001089:Arabinose; D050557:Catechol 2,3-Dioxygenase