title: Haematological effect of pulsed ultrasound in acute muscular inflammation in rats.
abstract: OBJECTIVE
  To evaluate the effect of low-intensity pulsed ultrasound (LIPUS) on the haematological dynamics of an acute inflammatory process after an iatrogenic muscular lesion.
  
  
  METHODS
  Controlled laboratory study.
  
  
  METHODS
  Research laboratory.
  
  
  METHODS
  Eighteen male Wistar rats (weight 350 to 450g) were submitted to surgical incision in the biceps femoris muscle (approximately 50%), and subsequently subdivided into control (n=9) and LIPUS (n=9) groups.
  
  
  METHODS
  Ultrasound (1.0MHz) was applied at the lesion site in the pulsed mode (2ms on, 8ms off) at 0.4W/cm(2) for 3 minutes at 1, 8 and 24 hours post-surgery.
  
  
  METHODS
  In these periods, blood was collected through venepuncture of the retro-orbital plexus and evaluated for different types of leukocytes and erythrocytes.
  
  
  RESULTS
  LIPUS reduced the total leukocyte count at 1, 8 and 24 hours post-surgery (1 hour, control 9017.2±481×10(3)/mm(3) vs LIPUS 6189.8±450×10(3)/mm(3); 8 hours, control 8078.2±501×10(3)/mm(3) vs LIPUS 5371.3±378×10(3)/mm(3); 24 hours, control 8192.3±646×10(3)/mm(3) vs LIPUS 6059.1±503×10(3)/mm(3); P<0.001). The monocyte count was reduced at 8 and 24 hours post-surgery (8 hours, control 815.5±126×10(3)/mm(3) vs LIPUS 375.4±70×10(3)/mm(3); 24 hours, control 875.3±124×10(3)/mm(3) vs LIPUS 564.7±56×10(3)/mm(3); P<0.001). The number of segmented neutrophils was only reduced at 1 hour post-surgery (control 5033.1±397×10(3)/mm(3) vs LIPUS 3594.8±191×10(3)/mm(3); P=0.006), and the lymphocyte count was only reduced at 8 hours post-surgery (control 4759.7±459×10(3)/mm(3) vs LIPUS 2584.1±356×10(3)/mm(3); P=0.003). Changes were not observed in the concentrations of young neutrophils, polibocytes and erythrocytes.
  
  
  CONCLUSIONS
  LIPUS reduced aspects of the inflammatory process following an acute incisional muscular lesion.
authors.affiliation: Department of Physiotherapy, University of Cruz Alta, RS, Brazil; Institute of Biological Sciences, Federal University of Rio Grande, RS, Brazil.
authors.forename: Luis Ulisses
authors.initials: LU
authors.lastname: Signori
authors.forename: Silvio Teixeira da
authors.initials: ST
authors.lastname: Costa
authors.forename: Adão Felix Saurin
authors.initials: AF
authors.lastname: Neto
authors.forename: Rodrigo Machado
authors.initials: RM
authors.lastname: Pizzolotto
authors.forename: Cristiane
authors.initials: C
authors.lastname: Beck
authors.forename: Graciele
authors.initials: G
authors.lastname: Sbruzzi
authors.forename: Antônio Marcos Vargas da
authors.initials: AM
authors.lastname: Silva
authors.forename: Rodrigo Della Méa
authors.initials: RD
authors.lastname: Plentz
mesh_terms: D000208:Acute Disease; D000818:Animals; D004195:Disease Models, Animal; D004906:Erythrocyte Count; D007049:Iatrogenic Disease; D007958:Leukocyte Count; D008297:Male; D018482:Muscle, Skeletal / Q000098:blood supply / Q000000981:diagnostic imaging / Q000276:immunology; D009220:Myositis / Q000097:blood / Q000000981:diagnostic imaging / Q000534:rehabilitation; D026741:Physical Therapy Modalities; D051381:Rats; D017208:Rats, Wistar; D012038:Regeneration; D014464:Ultrasonic Therapy; D014463:Ultrasonography; D014947:Wounds and Injuries / Q000097:blood / Q000000981:diagnostic imaging / Q000534:rehabilitation