title: Evaluation of the Xpert™ MRSA/SA Blood Culture assay for the detection of Staphylococcus aureus including strains with reduced vancomycin susceptibility from blood culture specimens.
abstract: The Xpert MRSA/SA Blood Culture (BC) assay (Cepheid, Sunnyvale, CA) was prospectively compared to culture and found to have excellent specificity for both Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) in BC specimens with a sensitivity of 75% (3/4) and 100% (17/17), respectively. Among 28 heterogeneous vancomycin-intermediate S. aureus (hVISA)/VISA spiked BCs, the assay correctly identified 84.6% VISA and 80% hVISA isolates as MRSA.
authors.affiliation: Microbiology, Austin Health, Victoria, Australia.
authors.forename: Peter G
authors.initials: PG
authors.lastname: Kelley
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Grabsch
authors.forename: Jenny
authors.initials: J
authors.lastname: Farrell
authors.forename: Shirley
authors.initials: S
authors.lastname: Xie
authors.forename: Janet
authors.initials: J
authors.lastname: Montgomery
authors.forename: Barrie
authors.initials: B
authors.lastname: Mayall
authors.forename: Benjamin P
authors.initials: BP
authors.lastname: Howden
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016470:Bacteremia / Q000382:microbiology*; D001431:Bacteriological Techniques / Q000379:methods*; D001769:Blood / Q000382:microbiology*; D024881:Drug Resistance, Bacterial; D006801:Humans; D008875:Middle Aged; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000302:isolation & purification*; D014640:Vancomycin / Q000494:pharmacology; D055815:Young Adult
chemical_list: D014640:Vancomycin