title: Chemical and biological assessment of Angelicae Sinensis Radix after processing with wine: an orthogonal array design to reveal the optimized conditions.
abstract: The roots of Angelica sinensis [Angelica Sinensis Radix (ASR)] have been used as a common health food supplement for women's care for thousands of years in China. According to Asian tradition, ASR could be processed with the treatment of wine, which subsequently promoted the biological functions of ASR. By chemical and biological assessments, an orthogonal array design was employed here to determine the roles of three variable parameters in the processing of ASR, including oven temperature, baking time, and flipping frequency. The results suggested that oven temperature and baking time were two significant factors, while flipping frequency was a subordinate factor. The optimized condition of processing with wine therefore was considered to be heating in an oven at 80 °C for 90 min with flipping twice per hour. Under the optimized processing conditions, the solubilities of ferulic acid and Z-ligustilide from ASR were markedly increased and decreased, respectively. In parallel, the biological functions of processed ASR were enhanced in both anti-platelet aggregation and estrogenic activation; these increased functions could be a result of the altered levels of ferulic acid and Z-ligustilide in wine-processed ASR. Thus, the chemical and biological assessment of the processed ASR was in full accordance with the Chinese old tradition.
authors.affiliation: Section of Marine Ecology and Biotechnology, Division of Life Science, and Center for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong, China.
authors.forename: Janis Y X
authors.initials: JY
authors.lastname: Zhan
authors.forename: Ken Y Z
authors.initials: KY
authors.lastname: Zheng
authors.forename: Kevin Y
authors.initials: KY
authors.lastname: Zhu
authors.forename: Cathy W C
authors.initials: CW
authors.lastname: Bi
authors.forename: Wendy L
authors.initials: WL
authors.lastname: Zhang
authors.forename: Crystal Y Q
authors.initials: CY
authors.lastname: Du
authors.forename: Qiang
authors.initials: Q
authors.lastname: Fu
authors.forename: Tina T X
authors.initials: TT
authors.lastname: Dong
authors.forename: Roy C Y
authors.initials: RC
authors.lastname: Choi
authors.forename: Karl W K
authors.initials: KW
authors.lastname: Tsim
authors.forename: David T W
authors.initials: DT
authors.lastname: Lau
mesh_terms: D029971:Angelica sinensis / Q000737:chemistry*; D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003296:Cooking / Q000295:instrumentation / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000494:pharmacology*; D006801:Humans; D010974:Platelet Aggregation / Q000187:drug effects; D011817:Rabbits; D013696:Temperature; D014920:Wine / Q000032:analysis
chemical_list: D004365:Drugs, Chinese Herbal