title: Isolation and identification of acaricidal compounds in Inula japonica (Asteraceae).
abstract: We identified acaricidal compounds in Inula japonica Thunberg (Asteraceae) that were effective against carmine spider mite, Tetranychus cinnabarinus (Boisduval). Petroleum-ether extracts from I. japonica were toxic to T. cinnabarinus, with a 50% lethal concentration (LC50) value of 1.18 mg/ml. Silica gel column chromatography was used to separate the acaricidal components. Seventeen of 38 fraction groups had mite mortality rates >50%, nine fraction groups had rates >60%, and three fraction groups had rates >80% at 2 mg/ml. The major volatile compounds in the bioactive fraction groups were identified by gas chromatography-mass spectroscopy, and beta-sitosterol (1), stigmasterol (2), lupeol (3), and alpha-amyrin (4) were identified and determined to have acaricidal activity against T. cinnabarinus in vitro.
authors.affiliation: Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture P. R. China, Beijing University of Agriculture Beijing, 102206, China.
authors.forename: D D
authors.initials: DD
authors.lastname: Duan
authors.forename: C Y
authors.initials: CY
authors.lastname: Bu
authors.forename: J
authors.initials: J
authors.lastname: Cheng
authors.forename: Y N
authors.initials: YN
authors.lastname: Wang
authors.forename: G L
authors.initials: GL
authors.lastname: Shi
mesh_terms: D056810:Acaricides / Q000302:isolation & purification*; D000818:Animals; D008401:Gas Chromatography-Mass Spectrometry; D038143:Inula / Q000737:chemistry*; D010840:Phytosterols / Q000302:isolation & purification; D010936:Plant Extracts / Q000737:chemistry; D040102:Tetranychidae; D018675:Toxicity Tests; D014315:Triterpenes / Q000302:isolation & purification
chemical_list: D056810:Acaricides; D010840:Phytosterols; D010936:Plant Extracts; D014315:Triterpenes