title: [Whole genome amplification and its application in forensic individual identification].
abstract: Whole genome amplification (WGA) is a technique that could non-selectively amplify the genomic DNA. In recent years, intensive studies have been made on application of WGA in trace DNA amplification, which might be applied to amplify trace DNA collected from crime scenes and provide enough DNA templates for forensic individual identification. However, the problem of amplification bias happened in the complicated test materials from real crime scenes is still troublesome. Development of WGA method with low amplification bias and high yield is the main target of current forensic research. Here, we reviewed the advances and the application prospect of WGA in individual identification. This might provide a reference for solving the problem of the amplification bias.
authors.affiliation: Laboratory of Molecular Genetics ofAging and Tumor, Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650224, China. caiseas@gmail.com
authors.forename: Hai-Qiang
authors.initials: HQ
authors.lastname: Cai
authors.forename: Hai-Tao
authors.initials: HT
authors.lastname: Liu
authors.forename: Bin
authors.initials: B
authors.lastname: Shi
authors.forename: An
authors.initials: A
authors.lastname: Li
authors.forename: Wen-Ru
authors.initials: WR
authors.lastname: Tang
authors.forename: Ying
authors.initials: Y
authors.lastname: Luo
mesh_terms: D004247:DNA / Q000235:genetics; D053803:Forensic Genetics / Q000379:methods*; D016678:Genome / Q000235:genetics*; D006801:Humans; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*
chemical_list: D004247:DNA