title: Asymmetric bioreduction of alkenes using ene-reductases YersER and KYE1 and effects of organic solvents.
abstract: Asymmetric trans-bioreduction of activated alkenes by KYE1 from Kluyveromyces lactis and Yers-ER from Yersinia bercovieri, two ene-reductases from the Old Yellow Enzyme family, showed a broad substrate spectrum with a moderate to excellent degree of stereoselectivity. Both substrate- and enzyme-based stereocontrols were observed to furnish opposite stereoisomeric products. The effects of organic solvents on enzyme activity and stereoselectivity were outlined in this study, where two-phase systems hexane and toluene are shown to sustain bioreduction efficiency even at high organic solvent content.
authors.affiliation: School of Chemical and Biomolecular Engineering, Parker H. Petit Institute of Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, Georgia 30332-0363, United States.
authors.forename: Yanto
authors.initials: Y
authors.lastname: Yanto
authors.forename: Christoph K
authors.initials: CK
authors.lastname: Winkler
authors.forename: Stephanie
authors.initials: S
authors.lastname: Lohr
authors.forename: Mélanie
authors.initials: M
authors.lastname: Hall
authors.forename: Kurt
authors.initials: K
authors.lastname: Faber
authors.forename: Andreas S
authors.initials: AS
authors.lastname: Bommarius
mesh_terms: D000475:Alkenes / Q000737:chemistry*; D007716:Kluyveromyces / Q000201:enzymology / Q000235:genetics; D009252:NADPH Dehydrogenase / Q000378:metabolism*; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism*; D012997:Solvents / Q000737:chemistry; D013237:Stereoisomerism; D014050:Toluene / Q000737:chemistry; D015007:Yersinia / Q000201:enzymology / Q000235:genetics
chemical_list: D000475:Alkenes; D012997:Solvents; D014050:Toluene; D010088:Oxidoreductases; D009252:NADPH Dehydrogenase