title: Effects of cold shock treatment on total lipid content and fatty acid composition of Aurantiochytrium limacinum strain mh0186.
abstract: To examine the effect of cold shock treatment on the fatty acid composition of Aurantiochytrium limacinum strain mh0186, a marine thraustochytrid, we cultivated this strain at 28°C for 72 h with shaking and stored the obtained biomass at 10°C for 72 h. A growth experiment was carried out for comparison, wherein strain mh0186 was grown at 10 and 15°C for 72 h with shaking, and it was found that the unsaturation of fatty acids was accelerated relative to that at 28°C. In the cold shock experiment, the total lipid content significantly increased during storage at 10°C for 72 h. Overall, the percentage of unsaturated fatty acids such as docosahexaenoic acid was almost stable while that of n-6 docosapentaenoic acid decreased slightly, but significantly, relative to that in the growth experiment.
authors.affiliation: Department of Biological Production and Environmental Science, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan.
authors.forename: Yousuke
authors.initials: Y
authors.lastname: Taoka
authors.forename: Naoki
authors.initials: N
authors.lastname: Nagano
authors.forename: Yuji
authors.initials: Y
authors.lastname: Okita
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Izumida
authors.forename: Shinichi
authors.initials: S
authors.lastname: Sugimoto
authors.forename: Masahiro
authors.initials: M
authors.lastname: Hayashi
mesh_terms: D000818:Animals; D018533:Biomass; D003001:Cloning, Molecular; D003080:Cold Temperature; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism*; D049851:Gastropoda / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism*; D008055:Lipids / Q000737:chemistry*
chemical_list: D005227:Fatty Acids; D008055:Lipids