title: The use of RNAi technologies for gene knockdown in zebrafish.
abstract: Despite being a popular and versatile model organism in which to study development and model disease, the use of zebrafish has been hampered by the lack of a reliable, stable and cost-effective method of gene knockdown. It is therefore not surprising that the discovery of RNAi as an exploitable method of post-transcriptional gene regulation has created a lot of excitement within the zebrafish research community. However, despite concerted efforts in the field, progress in the use of RNAi technologies in zebrafish has been extremely slow and a reliable means of RNAi-mediated gene knockdown remains elusive. The following reviews progress in the field to date, highlights the major pitfalls identified and suggests possible future directions.
authors.affiliation: Michael Smith Building, The University of Manchester, UK. adam.hurlstone@manchester.ac.uk
authors.forename: Amanda
authors.initials: A
authors.lastname: Kelly
authors.forename: Adam F
authors.initials: AF
authors.lastname: Hurlstone
mesh_terms: D000818:Animals; D055785:Gene Knockdown Techniques / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D015027:Zebrafish / Q000235:genetics*
chemical_list: D012330:RNA, Double-Stranded; D034741:RNA, Small Interfering