title: Crystallization and preliminary X-ray diffraction analysis of cyclic imide hydrolase (CIH) from Pseudomonas putida YZ-26.
abstract: A recombinant form of cyclic imide hydrolase from Pseudomonas putida YZ-26 has been crystallized by the hanging-drop method. X-ray diffraction data were collected to 1.9 Å resolution. The crystals belonged to the orthorhombic space group C222(1), with unit-cell parameters a = 111.91, b = 176.04, c = 176.06 Å. Assuming the presence of four molecules in the asymmetric unit gives a V(M) value of 3.10 Å(3) Da(-1) and a solvent content of 60.31%.
authors.affiliation: Core Facility Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China. fanzh@im.ac.cn
authors.forename: Zheng
authors.initials: Z
authors.lastname: Fan
authors.forename: Jianxun
authors.initials: J
authors.lastname: Qi
authors.forename: Yawei
authors.initials: Y
authors.lastname: Shi
authors.forename: Yiwei
authors.initials: Y
authors.lastname: Liu
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D006867:Hydrolases / Q000737:chemistry* / Q000378:metabolism; D007094:Imides / Q000378:metabolism; D016958:Pseudomonas putida / Q000201:enzymology*
chemical_list: D007094:Imides; D006867:Hydrolases