title: Gastroprotective activities of adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) on the growth of the stomach cancer AGS cell line and indomethacin-induced gastric ulcers.
abstract: Adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) seeds have long been used to treat warts, chapped skin, rheumatism, and neuralgia in traditional Chinese medicine (TCM). Recently, studies demonstrated its anti-inflammatory, antiproliferative, antitumor, and antiallergic activities. In the present study, we first report the gastroprotective effects of dehulled adlay (DA) seeds, which consist of bran (AB) and endosperm (AE). The DA ethanolic extract (DAE) was prepared, along with the AB and AE ethanolic extracts (ABE and AEE), and the inhibitory effects of these extracts were tested on the AGS gastric cancer cell line. Results indicated that the ABE showed better antiproliferative activity, and 19 compounds were purified from AB in a further phenolic-compound-guided separation. Among the isolated compounds, caffeic and chlorogenic acids significantly suppressed the growth of AGS cells. In addition, the antiulcer activity of DA was examined in an indomethacin-induced gastric lesion model. The ulcer index (UI) and oxidative biomarkers in animals decreased, while the non-protein sulfhydryl (NPSH) groups were elevated when given DA. This is the first investigation of antiulcer activity of adlay, and we demonstrated that the antioxidative-active phenolic acids in DA contribute to some portion of the gastroprotective effects.
authors.affiliation: Graduate Institute of Food Science and Technology, National Taiwan University, Taipei 106, Taiwan.
authors.forename: Cheng-Pei
authors.initials: CP
authors.lastname: Chung
authors.forename: Shih-Min
authors.initials: SM
authors.lastname: Hsia
authors.forename: Ming-Yi
authors.initials: MY
authors.lastname: Lee
authors.forename: Hong-Jhang
authors.initials: HJ
authors.lastname: Chen
authors.forename: Faiwen
authors.initials: F
authors.lastname: Cheng
authors.forename: Lu-Chi
authors.initials: LC
authors.lastname: Chan
authors.forename: Yueh-Hsiung
authors.initials: YH
authors.lastname: Kuo
authors.forename: Yun-Lian
authors.initials: YL
authors.lastname: Lin
authors.forename: Wenchang
authors.initials: W
authors.lastname: Chiang
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D031742:Coix / Q000737:chemistry*; D004195:Disease Models, Animal; D006801:Humans; D007213:Indomethacin / Q000009:adverse effects; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D020011:Protective Agents / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D013274:Stomach Neoplasms / Q000188:drug therapy / Q000503:physiopathology*; D013276:Stomach Ulcer / Q000139:chemically induced / Q000188:drug therapy* / Q000503:physiopathology
chemical_list: D010936:Plant Extracts; D020011:Protective Agents; D007213:Indomethacin