title: Isolated mouse inner cell mass is unable to reconstruct trophectoderm.
abstract: The ability of ICM to differentiate into TE is still a controversial issue. Many of authors have showed the reconstruction of TE from isolated ICMs. We showed that immunosurgical method is not 100% efficient and that the original TE cells very often remain on the surface of isolated ICMs. We also found that isolated ICM cells cultured in vitro do not express Cdx2, and that the TE is reconstituted from TE cells which have survived immunosurgery. This indicates that very soon after the formation of TE in the blastocyst, the cells of ICM lose the potency to differentiate into trophectoderm.
authors.affiliation: Department of Embryology, Institute of Zoology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland. kasiasz@biol.uw.edu.pl
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Szczepanska
authors.forename: Lukasz
authors.initials: L
authors.lastname: Stanczuk
authors.forename: Marek
authors.initials: M
authors.lastname: Maleszewski
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology*; D053624:Blastocyst Inner Cell Mass / Q000166:cytology* / Q000378:metabolism; D000071616:CDX2 Transcription Factor; D002454:Cell Differentiation; D002478:Cells, Cultured; D004475:Ectoderm / Q000166:cytology* / Q000196:embryology; D005260:Female; D018398:Homeodomain Proteins / Q000378:metabolism; D007150:Immunohistochemistry; D051379:Mice; D018613:Microscopy, Confocal; D014327:Trophoblasts / Q000166:cytology* / Q000378:metabolism
chemical_list: D000071616:CDX2 Transcription Factor; C491270:CDX2 protein, human; D018398:Homeodomain Proteins