title: TRIP6 and LPP, but not Zyxin, are present at a subset of telomeres in human cells.
abstract: The protection of chromosome ends requires the inhibition of DNA damage responses at telomeres. This inhibition is exerted in great part by the shelterin complex, known to prevent inappropriate ATM and ATR activation. The molecular mechanisms by which shelterin protects telomeres are incompletely understood. Recently, we have implicated for the first time a class of molecules, LIM domain proteins, in telomere protection. This protection occurred through interaction with shelterin, possibly through POT1, and required the pair of LIM proteins TRIP6 and LPP, themselves part of the Zyxin family. The domain similarity between TRIP6, LPP and Zyxin led us to ask whether the latter also interacted with telomeres. Here, we show that there is specificity in the association of LIM proteins with telomeres: Zyxin, despite a high degree of similarity with TRIP6 and LPP, was not detected at telomeres, nor found in a complex with shelterin. TRIP6 and LPP, however, were detected by immunofluorescence at a small subset of telomeres, perhaps those that are critically short. We speculate that specific LIM proteins are part of complex events occurring in the context of the telomere dysfunction response, and possibly at play during the induction of senescence.
authors.affiliation: Hunter College, New York, NY, USA.
authors.forename: Samantha A
authors.initials: SA
authors.lastname: Sheppard
authors.forename: Tatiana
authors.initials: T
authors.lastname: Savinova
authors.forename: Diego
authors.initials: D
authors.lastname: Loayza
mesh_terms: D000074183:ATPases Associated with Diverse Cellular Activities; D048868:Adaptor Proteins, Signal Transducing / Q000032:analysis* / Q000378:metabolism; D002460:Cell Line; D003598:Cytoskeletal Proteins / Q000032:analysis* / Q000378:metabolism; D004249:DNA Damage; D006801:Humans; D060588:LIM Domain Proteins / Q000032:analysis* / Q000378:metabolism; D046988:Proteasome Endopeptidase Complex; D011485:Protein Binding; D000089804:Shelterin Complex; D016615:Telomere / Q000737:chemistry* / Q000378:metabolism; D034501:Telomere-Binding Proteins / Q000378:metabolism; D014157:Transcription Factors / Q000032:analysis* / Q000378:metabolism; D060589:Zyxin / Q000032:analysis* / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D003598:Cytoskeletal Proteins; D060588:LIM Domain Proteins; C103594:LPP protein, human; C092282:PSMC5 protein, human; D000089804:Shelterin Complex; D034501:Telomere-Binding Proteins; D014157:Transcription Factors; C092842:ZYX protein, human; D060589:Zyxin; D046988:Proteasome Endopeptidase Complex; D000074183:ATPases Associated with Diverse Cellular Activities