title: [Effect of annexin 5 on StAR and testosterone synthesis related enzyme in rat Leydig cells].
abstract: OBJECTIVE
  To investigate the effect of annexin 5 on the expressions at mRNA levels and protein levels of StAR, P450scc, 3β-HSD, 17α-hydroxylase and 17β-HSD in rat Leydig cells.
  
  
  METHODS
  The primary rat Leydig cells were cultured for 24 h and then stimulated with 10(-9) mol/L annexin 5 for 12 h and 24 h respectively. Cellular total RNA and total protein were extracted respectively. The expressions of StAR, P450scc, 3β-HSD, and 17α-hydroxylase and 17β-HSD(10) mRNA were detected by reverse transcription-polymerase chain reaction (RT-PCR)and the protein levels were detected by Western blotting.
  
  
  RESULTS
  Compared with the control group, at the mRNA level, after being treated with annexin 5 for 12 h, only 17β-HSD(10) expression had a 26% increase (P<0.05) while the others had no significant difference. The expressions of StAR, P450scc and 3β-HSD elevated 55%, 69% and 59%(P<0.05) respectively, and 17β-HSD(10) increased 104%(P<0.01) while 17α-hydroxylase had no significant difference after being treated with annexin 5 for 24 h. At the protein level, after being treated with annexin 5 for 12 h, 17β-HSD expression had a 39% increase (P<0.05). After 24 h, P450scc, 3β-HSD and 17β-HSD elevated 35%, 88% (P<0.05) and 47% (P<0.01) respectively while StAR had no significant difference.
  
  
  CONCLUSIONS
  Annexin 5 regulates testosterone synthesis by affecting the expressions of P450scc, 3β-HSD and 17β-HSD at gene and protein levels.
authors.affiliation: Nanjing General Hospital of Nanjing Military Command, Nanjing 210002, China.
authors.forename: Xiao-qian
authors.initials: XQ
authors.lastname: Tao
authors.forename: Chai-ying
authors.initials: CY
authors.lastname: Lin
authors.forename: Hai-yan
authors.initials: HY
authors.lastname: Liu
authors.forename: Shan-shan
authors.initials: SS
authors.lastname: Shi
authors.forename: Rong
authors.initials: R
authors.lastname: Lu
authors.forename: Yu-feng
authors.initials: YF
authors.lastname: Huang
authors.forename: Xiao-jun
authors.initials: XJ
authors.lastname: Li
authors.forename: Yi-feng
authors.initials: YF
authors.lastname: Ge
authors.forename: Ying-xia
authors.initials: YX
authors.lastname: Cui
authors.forename: Bing
authors.initials: B
authors.lastname: Yao
mesh_terms: D015067:17-Hydroxysteroid Dehydrogenases / Q000378:metabolism*; D015096:3-Hydroxysteroid Dehydrogenases / Q000378:metabolism; D000818:Animals; D017304:Annexin A5 / Q000494:pharmacology*; D002478:Cells, Cultured; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000378:metabolism; D007985:Leydig Cells / Q000166:cytology / Q000378:metabolism*; D008297:Male; D010750:Phosphoproteins / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013739:Testosterone / Q000096:biosynthesis*; D000098682:Steroidogenic Acute Regulatory Protein
chemical_list: D017304:Annexin A5; D010750:Phosphoproteins; D000098682:Steroidogenic Acute Regulatory Protein; D013739:Testosterone; D015067:17-Hydroxysteroid Dehydrogenases; D015096:3-Hydroxysteroid Dehydrogenases; D002786:Cholesterol Side-Chain Cleavage Enzyme