title: Acetate repression of methane oxidation by supplemental Methylocella silvestris in a peat soil microcosm.
abstract: Methylocella spp. are facultative methanotrophs that grow on methane and multicarbon substrates, such as acetate. Acetate represses transcription of methane monooxygenase of Methylocella silvestris in laboratory culture. DNA stable-isotope probing (DNA-SIP) using (13)C-methane and (12)C-acetate, carried out with Methylocella-spiked peat soil, showed that acetate also repressed methane oxidation by Methylocella in environmental samples.
authors.affiliation: School of Life Sciences, University of Warwick, Coventry CV4 7AL, United Kingdom.
authors.forename: M Tanvir
authors.initials: MT
authors.lastname: Rahman
authors.forename: Andrew
authors.initials: A
authors.lastname: Crombie
authors.forename: Hélène
authors.initials: H
authors.lastname: Moussard
authors.forename: Yin
authors.initials: Y
authors.lastname: Chen
authors.forename: J Colin
authors.initials: JC
authors.lastname: Murrell
mesh_terms: D000085:Acetates / Q000378:metabolism*; D042062:Beijerinckiaceae / Q000378:metabolism*; D002247:Carbon Isotopes / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D007553:Isotope Labeling / Q000379:methods; D008697:Methane / Q000378:metabolism*; D010084:Oxidation-Reduction; D010105:Oxygenases / Q000378:metabolism; D012988:Soil Microbiology; D014158:Transcription, Genetic
chemical_list: D000085:Acetates; D002247:Carbon Isotopes; D010105:Oxygenases; C021555:methane monooxygenase; D008697:Methane