title: Studies on in vitro degradability of mixed crude enzyme extracts produced from Pleurotus spp.
abstract: A preliminary investigation was conducted to assess lignocellulolytic efficiency of crude extracts from three white-rot fungi, Pleurotus florida PF05 (PF), Pleurotus sajor-caju PS07 (PS) and Pleurotus eryngii PE08 (PE). The activities of CMC-ase, xylanase, beta-glucosidase, beta-xylosidase, laccase and Mn peroxidase in extracts were evaluated. PF produced its highest CMC-ase (317 UL(-1)'), beta-glucosidase (62 UL(-1)), beta-xylosidase (37 UL(-1)) and laccase (347 UL(-1)) activities while, PS produced highest xylanase (269 UL-(1)) and Mn peroxidase (69 UL(-1)) activities. In addition, crude extracts extracted were employed for their in vitro degradability assessment; and were evaluated with mono and mixed extracts separately to corn cob substrate. The losses in cell wall components and dry matter during 5 and 10 days incubations were analyzed after treatments of extracts. Maximum 8.2, 4.4 and 2.8% loss were found respectivelyin hemicellulose (HC), cellulose (C) and lignin (L) with mono extract of PF within 10 days. The influence of mono extract of each strain (PF PS and PE) and their mixed extracts (PF+PS, PF+PE, PS+PE and PF+PS+PE) on degradation of cell wall constituents were remarkably differed. The mixed extract treatment proved maximum 13.6% HC loss by PF+PS+PE extract, 9.2% loss in C by PF+PS extract and 5.2% loss of L by the PF+PS+PE extract treatment. The highest dry matter loss (8.2%) was recorded with PF+PS+PE mixed extract combination.
authors.affiliation: Department of Microbiology, Institute of Bioscience and Biotechnology, Chhatrapati Sahu Ji Mahraj University, Kanpur--208 024, India. ramnarain_itrc@rediffmail.com
authors.forename: Ram
authors.initials: R
authors.lastname: Naraian
authors.forename: Dharam
authors.initials: D
authors.lastname: Singh
authors.forename: Anju
authors.initials: A
authors.lastname: Verma
authors.forename: S K
authors.initials: SK
authors.lastname: Garg
mesh_terms: D002473:Cell Wall / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry*; D020076:Pleurotus / Q000737:chemistry* / Q000201:enzymology*
chemical_list: D010936:Plant Extracts