title: Transformation of wheat with the HMW-GS 1Bx14 gene without markers.
abstract: High molecular weight (HMW) glutenin polypeptides are critical contributors to the visco/elastic properties responsible for the processing characteristics and utilizations of wheat flour. In order to improve bread making quality of flour and produce transgenic plants free of selectable markers, a linear DNA construct consisting of a minimal expression cassette with the HMW-GS 1Bx14 gene was transformed into wheat cultivar Mianyang 19 by microprojectile bombardment. The transformants were selected by PCR instead of herbicidal markers. Seven transgenic plants were identified from a total of 1219 transformants, yielding a transformation frequency of 0.28%. An SDS-PAGE analysis confirmed that the 1Bx14 gene was expressed in three T1 seeds of the transgenic plants. Our results demonstrated that it is feasible to obtain marker-free transformants using the linear-expression-cassette-transformation approach coupled with PCR selection.
authors.affiliation: College of Life Science, Northwest A&F University, Yangling, Shaanxi, 712100 China.
authors.forename: X
authors.initials: X
authors.lastname: Liu
authors.forename: W
authors.initials: W
authors.lastname: Jin
authors.forename: J
authors.initials: J
authors.lastname: Liu
authors.forename: H
authors.initials: H
authors.lastname: Zhao
authors.forename: A
authors.initials: A
authors.lastname: Guo
mesh_terms: D017343:Genes, Plant; D005819:Genetic Markers; D014170:Transformation, Genetic; D014908:Triticum / Q000235:genetics* / Q000254:growth & development
chemical_list: D005819:Genetic Markers