title: Automated radiosynthesis of the thiol-reactive labeling agent N-[6-(4-[18F]fluorobenzylidene)aminooxyhexyl]maleimide ([18F]FBAM).
abstract: The two-step radiosynthesis of N-[6-(4-[(18)F]fluorobenzylidene)aminooxyhexyl]maleimide ([(18)F]FBAM) was adapted to a remotely controlled synthesizer module. After optimization of reaction conditions as well as solid phase extraction based purification steps, the final [(18)F]FBAM was obtained in a decay-corrected radiochemical yield of 29±4% (related to [(18)F]fluoride, n=12) within a total synthesis time of 40 min. The radiochemical purity of [(18)F]FBAM was in the range 94-98%, the specific activity was determined with 13.4-17.2 GBq/μmol.
authors.affiliation: Institute of Radiopharmacy, Forschungszentrum Dresden-Rossendorf e.V., Germany. t.kniess@fzd.de
authors.forename: Torsten
authors.initials: T
authors.lastname: Kniess
authors.forename: Manuela
authors.initials: M
authors.lastname: Kuchar
authors.forename: Jens
authors.initials: J
authors.lastname: Pietzsch
mesh_terms: D057205:Automation, Laboratory / Q000379:methods; D005462:Fluorine Radioisotopes; D007553:Isotope Labeling / Q000379:methods*; D008301:Maleimides / Q000138:chemical synthesis*; D019275:Radiopharmaceuticals / Q000138:chemical synthesis*; D013439:Sulfhydryl Reagents / Q000138:chemical synthesis*
chemical_list: D005462:Fluorine Radioisotopes; D008301:Maleimides; C518594:N-(6-(4-fluorobenzylidene)aminooxyhexyl)maleimide; D019275:Radiopharmaceuticals; D013439:Sulfhydryl Reagents