title: Interaction between CTLA4 gene and IBD5 locus in Hungarian Crohn's disease patients.
abstract: OBJECTIVE
  The IGR2198a_1 and IGR2096a_1 variants of the IBD5 region were found to be associated with Crohn's disease (CD) in the Hungarian population, while IGR2230a_1 does not seem to confer risk for the disease. In the present study, our aim was to investigate the statistical interaction of these three IBD5 polymorphisms with the +49 A/G substitution within the cytotoxic T lymphocyte antigen-4 (CTLA4) gene, detected previously as neutral gene variant in Hungarian IBD patients.
  
  
  METHODS
  A total of 305 unrelated subjects with CD and 310 healthy controls were genotyped with PCR-RFLP methods.
  
  
  RESULTS
  In contrast with single gene effects, after genotype stratification, the IGR2198a_1 C and IGR2096a_1 T variants were found to confer susceptibility only in subjects with CTLA4 +49 AA genotype (P = 0.008; OR = 1.86 and P = 0.016; OR = 1.74, respectively), for IGR2230a_1 no such effect on disease risk could be demonstrated.
  
  
  CONCLUSIONS
  Analysis of specific genotype combinations unfolded a possible association between the CTLA4 +49 A/G substitution and two of the observed IBD5 variants with respect to disease risk.
authors.affiliation: Department of Medical Genetics, University of Pécs, Pécs, Szigeti út 12, H-7624, Hungary.
authors.forename: Veronika
authors.initials: V
authors.lastname: Csöngei
authors.forename: Luca
authors.initials: L
authors.lastname: Járomi
authors.forename: Eniko
authors.initials: E
authors.lastname: Sáfrány
authors.forename: Csilla
authors.initials: C
authors.lastname: Sipeky
authors.forename: Lili
authors.initials: L
authors.lastname: Magyari
authors.forename: Noémi
authors.initials: N
authors.lastname: Polgár
authors.forename: Judit
authors.initials: J
authors.lastname: Bene
authors.forename: Patrícia
authors.initials: P
authors.lastname: Sarlós
authors.forename: Lilla
authors.initials: L
authors.lastname: Lakner
authors.forename: Eszter
authors.initials: E
authors.lastname: Baricza
authors.forename: Melinda
authors.initials: M
authors.lastname: Szabó
authors.forename: Gábor
authors.initials: G
authors.lastname: Rappai
authors.forename: Béla
authors.initials: B
authors.lastname: Melegh
mesh_terms: D000328:Adult; D060908:CTLA-4 Antigen / Q000235:genetics*; D016022:Case-Control Studies; D003424:Crohn Disease / Q000235:genetics*; D004843:Epistasis, Genetic; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D056426:Genetic Loci / Q000235:genetics*; D020022:Genetic Predisposition to Disease; D006801:Humans; D006814:Hungary; D008297:Male; D012307:Risk Factors
chemical_list: D060908:CTLA-4 Antigen; C556706:CTLA4 protein, human