title: Proteomic evaluation of cancer cells: identification of cell surface proteins.
abstract: The plasma membrane proteome can be defined as the entire complement of proteins present in the plasma membrane at a specific time. The process of carcinogenesis leads to changes in the array of proteins present in the plasma membrane proteome. Analysis of differential expression of such proteins in cancer is extremely important; due to their position on the cell surface they have a potential for use as diagnostic and/or prognostic markers and therapeutic targets. Biotin labelling followed by avidin chromatography can be used to obtain membrane protein enriched lysates from cell lines, which can then be resolved using SDS-PAGE, coomassie staining and mass spectrometry.
authors.affiliation: Cancer Sciences Division, School of Medicine, University of Southampton, Southampton, UK. Samantha.Larkin@kcl.ac.uk
authors.forename: Samantha
authors.initials: S
authors.lastname: Larkin
authors.forename: Claire
authors.initials: C
authors.lastname: Aukim-Hastie
mesh_terms: D001710:Biotin / Q000378:metabolism; D002457:Cell Extracts; D045744:Cell Line, Tumor; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000302:isolation & purification / Q000378:metabolism*; D011467:Prostate / Q000166:cytology / Q000473:pathology; D011471:Prostatic Neoplasms / Q000378:metabolism / Q000473:pathology*; D040901:Proteomics / Q000379:methods*; D013194:Staining and Labeling
chemical_list: D002457:Cell Extracts; D008565:Membrane Proteins; D001710:Biotin