title: Highly sensitive, quick and simple quantification method for mono and disaccharides in aqueous media using liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry (LC-APCI-MS).
abstract: A highly sensitive, rapid LC-APCI-MS method for identification and quantification of mono and disaccharides in simple or complex aqueous phase has been developed. This original method is easy to use, no derivation and no post-column injection are needed. The separation is performed with a hydrophilic amino interaction (HILIC) column allowing high-throughput analysis with analysis times of 15 min for monosaccharides to 22 min for disaccharides. The development of the method carried out with 9 standard saccharides allowed to point out a dynamic range from 0.1-25.6 to 1-256 μg mL(-1) depending on the considered sugar. Next, the method was validated on saccharides at known concentrations in water and on 2 real samples: orange juice and aqueous phase obtained after enzymatic hydrolysis of sunflower seeds.
authors.affiliation: Laboratoire d'Ingénierie des Biomolécules (LIBio) Nancy-Université, Vandoeuvre lès Nancy Cedex, France.
authors.forename: G
authors.initials: G
authors.lastname: Ricochon
authors.forename: C
authors.initials: C
authors.lastname: Paris
authors.forename: M
authors.initials: M
authors.lastname: Girardin
authors.forename: L
authors.initials: L
authors.lastname: Muniglia
mesh_terms: D001628:Beverages / Q000032:analysis; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D004187:Disaccharides / Q000032:analysis* / Q000302:isolation & purification; D006368:Helianthus / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D009005:Monosaccharides / Q000032:analysis* / Q000302:isolation & purification; D010936:Plant Extracts / Q000737:chemistry; D012015:Reference Standards; D015203:Reproducibility of Results; D012639:Seeds / Q000737:chemistry; D012680:Sensitivity and Specificity; D014867:Water / Q000737:chemistry
chemical_list: D004187:Disaccharides; D009005:Monosaccharides; D010936:Plant Extracts; D014867:Water