title: Human papilloma virus detection in neoplastic and non-neoplastic nasopharyngeal tissues in Taiwan.
abstract: BACKGROUND
  Human papilloma virus (HPV) has been implicated in the carcinogenesis and prognosis of certain head and neck cancers. Whether it also has a role in the pathogenesis of nasopharyngeal carcinoma (NPC) in Taiwan is unclear.
  
  
  METHODS
  Detection and genotyping of HPVs were performed in 43 primary NPCs (one WHO-I and 42 WHO-II/III) and 40 nasopharyngeal controls using PCR-based HPV genotyping arrays. Localisation of high-risk HPV and Epstein-Barr virus genomes was performed in another 46 primary NPCs (five WHO-I and 41 WHO-II/III) and seven paired metastatic WHO-II/III NPCs using in situ hybridisation.
  
  
  RESULTS
  In the HPV genotyping cohort, oncogenic HPVs were detected equally in WHO-II/III NPCs (31%, 13/42) and nasopharyngeal controls (35%, 14/40). Tumour high-risk HPV status did not correlate with the prognosis of patients with NPC. In the high-risk HPV in situ hybridisation cohort, 14 (88%) of the 16 oncogenic HPV-positive WHO-II/III NPCs showed a unique cytoplasmic/perinuclear staining pattern, which is distinct from the typical dot/punctate nuclear staining pattern indicating HPV genome integration. In addition, oncogenic HPVs were not always retained in NPC cells during the process of metastasis.
  
  
  CONCLUSIONS
  This study does not support an association between oncogenic HPV and the carcinogenesis or prognosis of WHO-II/III NPCs in Taiwan.
authors.affiliation: Department of Otolaryngology, Medical College and Hospital, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Cheng-Chih
authors.initials: CC
authors.lastname: Huang
authors.forename: Jenn-Ren
authors.initials: JR
authors.lastname: Hsiao
authors.forename: Ming-Wei
authors.initials: MW
authors.lastname: Yang
authors.forename: Yuan-Hua
authors.initials: YH
authors.lastname: Wu
authors.forename: Keng-Fu
authors.initials: KF
authors.lastname: Hsu
authors.forename: Yao
authors.initials: Y
authors.lastname: Chang
authors.forename: Chaio-Wei
authors.initials: CW
authors.lastname: Chen
authors.forename: Sen-Tien
authors.initials: ST
authors.lastname: Tsai
authors.forename: Hsuan-Pei
authors.initials: HP
authors.lastname: Wei
authors.forename: Ying-Tai
authors.initials: YT
authors.lastname: Jin
mesh_terms: D002277:Carcinoma; D020031:Epstein-Barr Virus Infections / Q000453:epidemiology; D005260:Female; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D008875:Middle Aged; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000473:pathology / Q000821:virology*; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000150:complications / Q000453:epidemiology*; D013624:Taiwan / Q000453:epidemiology