title: Feeder layers: co-culture with nonneoplastic cells.
abstract: Maintenance of a mitotically inactive feeder layer which is able to provide extracellular matrix and growth factors can be critical in establishing and maintaining primary tumor cells. How feeder cells are handled and processed is crucial for providing trouble-free support for primary tumor cells and spontaneously immortalized lines.
authors.affiliation: Centre for Oncology and Molecular Medicine, University of Dundee, Ninewells Hospital & Medical School, Dundee, UK.
authors.forename: Celine
authors.initials: C
authors.lastname: Pourreyron
authors.forename: Karin J
authors.initials: KJ
authors.lastname: Purdie
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Watt
authors.forename: Andrew P
authors.initials: AP
authors.lastname: South
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002470:Cell Survival; D018920:Coculture Techniques / Q000379:methods*; D051379:Mice; D008938:Mitosis