title: Osteosarcoma amplified 9 is highly expressed in mouse adipocytes and controls lipid storage.
abstract: Adipocytes are among the most important cells for energy storage, metabolism and balance in mammals. Dysfunctional adipocytes are closely associated with type 2 diabetes and obesity, and thus with insulin resistance and inflammation. Recent studies show that endoplasmic reticulum (ER) stress may be responsible for obesity-induced insulin resistance. In this study, we investigated an ER-associated degradation-related protein in adipocytes, namely osteosarcoma amplified 9 (OS-9), a protein that is reported to have lectin activity and to be involved in ER quality control. We found that OS-9 is expressed at high levels in both types of fat tissues - brown and white adipose tissues - and we examined the subcellular localization of OS-9 in 3T3-L1 adipocytes. Furthermore, we observed that the expression levels of the OS-9 protein gradually increased as 3T3-L1 adipocytes differentiated. Most notably, we found that the depletion of this protein influences adipocyte differentiation and lipid storage, although the mechanism behind this is unclear.
authors.affiliation: Institute of Cell Biology, Beijing Normal University, Beijing 100875, People's Republic of China.
authors.forename: Luxin
authors.initials: L
authors.lastname: Yu
authors.forename: Zongqian
authors.initials: Z
authors.lastname: Nian
authors.forename: Jianli
authors.initials: J
authors.lastname: Sang
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000378:metabolism*; D000818:Animals; D002454:Cell Differentiation; D004721:Endoplasmic Reticulum / Q000378:metabolism; D037102:Lectins / Q000032:analysis / Q000037:antagonists & inhibitors / Q000378:metabolism*; D050356:Lipid Metabolism; D051379:Mice; D009363:Neoplasm Proteins / Q000032:analysis / Q000037:antagonists & inhibitors / Q000378:metabolism*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism
chemical_list: D037102:Lectins; D009363:Neoplasm Proteins; C561422:OS9 protein, mouse; D034741:RNA, Small Interfering