title: Oviduct-specific enhanced green fluorescent protein expression in transgenic chickens.
abstract: In this study, we confirmed the ability of the 2-kb promoter fragment of the chicken ovalbumin gene to drive tissue-specific expression of a foreign EGFP gene in chickens. Recombinant lentiviruses containing the EGFP gene were injected into the subgerminal cavity of 539 freshly laid embryos (stage X). Subsequently the embryos were incubated to hatch using phases II and III of the surrogate shell ex vivo culture system. Twenty-four chicks (G0) were hatched and screened for EGFP with PCR. Two chicks were identified as transgenic birds (G1), and these founders were mated with wild-type chickens to generate transgenic progeny. In the generated transgenic hens (G2), EGFP was expressed specifically in the tubular gland of the oviduct. These results show the potential of the chicken ovalbumin promoter for the production of biologically active proteins in egg white.
authors.affiliation: Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Republic of Korea.
authors.forename: Sung June
authors.initials: SJ
authors.lastname: Byun
authors.forename: Sung Woo
authors.initials: SW
authors.lastname: Kim
authors.forename: Kyung-Woon
authors.initials: KW
authors.lastname: Kim
authors.forename: Jeom Sun
authors.initials: JS
authors.lastname: Kim
authors.forename: In-Sul
authors.initials: IS
authors.lastname: Hwang
authors.forename: Hee Kyoung
authors.initials: HK
authors.lastname: Chung
authors.forename: In Sun
authors.initials: IS
authors.lastname: Kan
authors.forename: Ik-Soo
authors.initials: IS
authors.lastname: Jeon
authors.forename: Won-Kyoung
authors.initials: WK
authors.lastname: Chang
authors.forename: Soo-Bong
authors.initials: SB
authors.lastname: Park
authors.forename: Jae Gyu
authors.initials: JG
authors.lastname: Yoo
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D002645:Chickens / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics*; D016086:Lentivirus / Q000235:genetics; D009928:Organ Specificity; D010047:Ovalbumin / Q000235:genetics; D010057:Oviducts / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics
chemical_list: C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D010047:Ovalbumin