title: Osteopontin modulates inflammation, mucin production, and gene expression signatures after inhalation of asbestos in a murine model of fibrosis.
abstract: Inflammation and lung remodeling are hallmarks of asbestos-induced fibrosis, but the molecular mechanisms that control these events are unclear. Using laser capture microdissection (LCM) of distal bronchioles in a murine asbestos inhalation model, we show that osteopontin (OPN) is up-regulated by bronchiolar epithelial cells after chrysotile asbestos exposures. In contrast to OPN wild-type mice (OPN(+/+)) inhaling asbestos, OPN null mice (OPN(-/-)) exposed to asbestos showed less eosinophilia in bronchoalveolar lavage fluids, diminished lung inflammation, and decreased mucin production. Bronchoalveolar lavage fluid concentrations of inflammatory cytokines (IL-1β, IL-4, IL-6, IL-12 subunit p40, MIP1α, MIP1β, and eotaxin) also were significantly less in asbestos-exposed OPN(-/-) mice. Microarrays performed on lung tissues from asbestos-exposed OPN(+/+) and OPN(-/-) mice showed that OPN modulated the expression of a number of genes (Col1a2, Timp1, Tnc, Eln, and Col3a1) linked to fibrosis via initiation and cross talk between IL-1β and epidermal growth factor receptor-related signaling pathways. Novel targets of OPN identified include genes involved in cell signaling, immune system/defense, extracellular matrix remodeling, and cell cycle regulation. Although it is unclear whether the present findings are specific to chrysotile asbestos or would be observed after inhalation of other fibers in general, these results highlight new potential mechanisms and therapeutic targets for asbestosis and other diseases (asthma, smoking-related interstitial lung diseases) linked to OPN overexpression.
authors.affiliation: Department of Environmental Health Sciences and NanoCenter, University of South Carolina, Columbia, South Carolina, USA. sabo@phhp.ufl.edu
authors.forename: Tara
authors.initials: T
authors.lastname: Sabo-Attwood
authors.forename: Maria E
authors.initials: ME
authors.lastname: Ramos-Nino
authors.forename: Maria
authors.initials: M
authors.lastname: Eugenia-Ariza
authors.forename: Maximilian B
authors.initials: MB
authors.lastname: Macpherson
authors.forename: Kelly J
authors.initials: KJ
authors.lastname: Butnor
authors.forename: Pamela C
authors.initials: PC
authors.lastname: Vacek
authors.forename: Sean P
authors.initials: SP
authors.lastname: McGee
authors.forename: Jessica C
authors.initials: JC
authors.lastname: Clark
authors.forename: Chad
authors.initials: C
authors.lastname: Steele
authors.forename: Brooke T
authors.initials: BT
authors.lastname: Mossman
mesh_terms: D000818:Animals; D017632:Asbestos, Serpentine / Q000009:adverse effects; D001195:Asbestosis / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D055745:Bronchioles / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D018893:Bronchoalveolar Lavage; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D007249:Inflammation / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D007834:Lasers; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D042282:Microdissection; D009077:Mucins / Q000096:biosynthesis*; D053495:Osteopontin / Q000235:genetics / Q000378:metabolism*; D020545:Respiratory Mucosa / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D015854:Up-Regulation
chemical_list: D017632:Asbestos, Serpentine; D009077:Mucins; C506896:Spp1 protein, mouse; D053495:Osteopontin