title: [Ultraviolet spectrophotometric determination of tenoxicam using iodine solution as reagent].
abstract: For the tenoxicam determination (Nonsteroidal Antiinflammatory Drug - NSAID) it was developed a spectrophotometric method, after the coupling reaction of tenoxicam with iodine, in methanolic medium, with maximum of absorbance at 289 nm.
  
  
  METHODS
  The practical working conditions were established. In the 0.5 divided by 5.0 microg/mL range of tenoxicam concentration, were used 5 x 10(-3) M iodine solution and 0.5 N hydrocloric acid. The stability of product were evaluated for 30 minutes. The developed method was validated.
  
  
  RESULTS
  The method showed a good linearity in the range of 0.5 divided 5.0 microg/mL (the correlation coefficient r = 0.9995). The detection limit (LD) was 0.14 microg/mL and the quantification limit (LQ) was 0.49 microg/mL. There were established the precison (RSD = 1.90%) and the accuracy-recovery in the range 97.27 divided by 102.56% with a mean recovery of 99.49%.
  
  
  CONCLUSIONS
  The experimental results demonstrated a good sensibility. The specific absorptivity for this method is A1%(1 cm,289 nm) = 1770 much higher than tenoxicam in methanol (A1%(1 cm,360 nm) = 323).
authors.affiliation: Institutul Naţional de Cercetare-Dezvoltare pentru Microbiologie si Imunologie I. Cantacuzino filiala Iaşi.
authors.forename: Mariana
authors.initials: M
authors.lastname: Mândrescu
authors.forename: A F
authors.initials: AF
authors.lastname: Spac
authors.forename: V
authors.initials: V
authors.lastname: Dorneanu
mesh_terms: D000891:Anti-Infective Agents, Local / Q000737:chemistry*; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry*; D006851:Hydrochloric Acid / Q000737:chemistry; D007202:Indicators and Reagents / Q000737:chemistry; D007455:Iodine / Q000737:chemistry*; D057230:Limit of Detection; D000432:Methanol / Q000737:chemistry; D010894:Piroxicam / Q000031:analogs & derivatives* / Q000737:chemistry; D015203:Reproducibility of Results; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000891:Anti-Infective Agents, Local; D000894:Anti-Inflammatory Agents, Non-Steroidal; D007202:Indicators and Reagents; D012996:Solutions; D010894:Piroxicam; D007455:Iodine; D006851:Hydrochloric Acid; D000432:Methanol; C032801:tenoxicam