title: [Identification of ubiquitously expressed transcript as the potential interactor of hepatitis B virus polymerase].
abstract: OBJECTIVE
  To investigate the function of hepatitis B virus polymerase (HBV Pol) in the viral life cycle by screening the proteins interacting with HBV polymerase.
  
  
  METHODS
  The HBV Pol gene was constructed into the pGBKT7 vector. GAL4 yeast two-hybrid system was used to screen the human liver cDNA library to obtain proteins which interacted with HBV Pol. GST-pull down assay was applied to confirm the protein interactions.
  
  
  RESULTS
  Ubiquitously expressed transcript (UXT) was selected by the yeast two-hybrid system. GST-pull down assay confirmed the in vitro interaction between HBV Pol and UXT.
  
  
  CONCLUSIONS
  UXT is a potential interactor of HBV Pol, and this protein interaction may provide clues of the function of HBV Pol in HBV life cycle.
authors.affiliation: Department of Bioengineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.
authors.forename: Meng-Zhang
authors.initials: MZ
authors.lastname: Chen
authors.forename: Yi
authors.initials: Y
authors.lastname: Zou
authors.forename: Jun-Wu
authors.initials: JW
authors.lastname: Li
authors.forename: Yue-Qin
authors.initials: YQ
authors.lastname: Li
authors.forename: Hong-Jian
authors.initials: HJ
authors.lastname: Li
authors.forename: Tian-Hong
authors.initials: TH
authors.lastname: Zhou
mesh_terms: D018797:Cell Cycle Proteins; D015684:Gene Products, pol / Q000378:metabolism*; D006515:Hepatitis B virus / Q000201:enzymology*; D006801:Humans; D018832:Molecular Chaperones; D009363:Neoplasm Proteins / Q000378:metabolism*; D025941:Protein Interaction Mapping; D020798:Two-Hybrid System Techniques; D014779:Virus Replication
chemical_list: D018797:Cell Cycle Proteins; D015684:Gene Products, pol; D018832:Molecular Chaperones; D009363:Neoplasm Proteins; C066027:P protein, Hepatitis B virus; C118920:UXT protein, human