title: Immunoassay method to check the flagellin mediated binding of stenotrophomonas maltophilia to polystyrene.
abstract: Plate count and spectrophotometric methods have been used to asses the ability of an organism to attach to different surfaces and form biofilms. In the present study we report a highly sensitive, specific and quick method to check the role of flagellin in bacterial adhesion to polystyrene. Flagellin from Stenotrophomonas maltophilia showed high affinity for polystyrene (P < 0.05), which decreased on pretreatment offlagellin with anti-flagellin in a dilution dependent manner. In an enzyme immunoassay format a positive correlation was detected between the anti-flagellin dilutions and flagellin attachment to polystyrene (correlation coefficient +0.860155). These evidences conclusively prove the involvement of flagella in the adhesion ofS. maltophilia to polystyrene surface and enzyme immunoassay, a quick and reliable method to check this phenomenon.
authors.affiliation: Department of Microbiology, Panjab University, Chandigarh, 160014, India.
authors.forename: Ayaid K
authors.initials: AK
authors.lastname: Zgair
authors.forename: Sanjay
authors.initials: S
authors.lastname: Chhibber
mesh_terms: D000907:Antibodies, Bacterial / Q000737:chemistry* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005408:Flagellin / Q000032:analysis* / Q000276:immunology; D011137:Polystyrenes / Q000737:chemistry; D020615:Stenotrophomonas maltophilia / Q000737:chemistry* / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D011137:Polystyrenes; D005408:Flagellin