title: Lactic acid bacteria isolated from young calves--characterization and potential as probiotics.
abstract: Lactic acid bacteria (LAB) are widely used as probiotics in humans and animals to restore the ecological balance of different mucosa. They help in the physiological functions of newborn calves that are susceptible to a variety of syndromes. The criteria for the selection of strains for the design of probiotic products are not available. Based in the host-specificity of the indigenous microbiota, 96 LAB isolates from faeces and oral cavity of calves were obtained. The surface properties were screened showing a small number of highly hydrophobic or autoagglutinating isolates. Also, a group produced H(2)O(2) and were able to inhibit pathogens, and two strains were bacteriocin-producers. Some grew at very low pH and high bile concentrations. The strains sharing some of the specific properties evaluated were identified genetically, assayed their compatibility and exopolysaccharide production. The results allow going further in the establishment of criteria to select strains to be included in a multi-strain-probiotic-product to be further assayed in animals.
authors.affiliation: Centro de Referencia para Lactobacilos (CERELA-CONICET), Chacabuco 145, 4000 San Miguel de Tucumán, Argentina.
authors.forename: Natalia C
authors.initials: NC
authors.lastname: Maldonado
authors.forename: Clara Silva
authors.initials: CS
authors.lastname: de Ruiz
authors.forename: María Claudia
authors.initials: MC
authors.lastname: Otero
authors.forename: Fernando
authors.initials: F
authors.lastname: Sesma
authors.forename: María Elena
authors.initials: ME
authors.lastname: Nader-Macías
mesh_terms: D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn / Q000382:microbiology; D001430:Bacteriocins / Q000235:genetics / Q000302:isolation & purification; D002417:Cattle; D007778:Lactobacillus / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction / Q000662:veterinary; D019936:Probiotics / Q000302:isolation & purification*; D013499:Surface Properties
chemical_list: D001430:Bacteriocins