title: [Breeding strains of Panax notoginseng by using EST-SSR markers].
abstract: OBJECTIVE
  To comparatively determine the genetic variation and differentiation of different breeding strains of Panax notoginseng for providing the basic information for genetic breeding.
  
  
  METHODS
  The genetic diversity and genetic structure of the 17 breeding strains of P. notoginseng were assayed by using EST-SSR molecular marker.
  
  
  RESULTS
  A total of 136 polymorphic loci of EST-SSR were detected in the 17 breeding strains of P. notoginseng, with the PIC (polymorphism information content) being 0.78, H (the gene diversity within population) being 0.139, the I (the Shannon's information index) being 0.208. Gst (coefficient of gene differentiation) was 0.382 among the 17 strains. The cluster analysis of genetic similarity showed that the 17 strains of P. notoginseng and P. stipuleanatus were classified into 4 groups, while the 17 strains of P. notoginseng were classified into three subgroups.
  
  
  CONCLUSIONS
  The genetic differentiation was detected among the 17 strains of P. notoginseng from the same cultivation population by bulk selecting. And it was feasible to detect the effect of bulk selection by EST-SSR markers.
authors.affiliation: Institute of Medicinal Plant, Yunnan Academy of Agricultural Sciences, Kunming 650223, China. zjy_wb740921@sina.com
authors.forename: Jinyu
authors.initials: J
authors.lastname: Zhang
authors.forename: Weize
authors.initials: W
authors.lastname: Yang
authors.forename: Xiuming
authors.initials: X
authors.lastname: Cui
authors.forename: Hong
authors.initials: H
authors.lastname: Yu
authors.forename: Hang
authors.initials: H
authors.lastname: Jin
authors.forename: Zhongjian
authors.initials: Z
authors.lastname: Chen
authors.forename: Tao
authors.initials: T
authors.lastname: Shen
mesh_terms: D001947:Breeding; D020224:Expressed Sequence Tags; D014644:Genetic Variation; D018895:Microsatellite Repeats; D053723:Panax notoginseng / Q000145:classification / Q000235:genetics* / Q000502:physiology