title: Cloning, overexpression, purification, crystallization and preliminary X-ray diffraction analysis of an inositol monophosphatase family protein (SAS2203) from Staphylococcus aureus MSSA476.
abstract: The gene product of the sas2203 ORF of Staphylococcus aureus MSSA476 encodes a 30 kDa molecular-weight protein with a high sequence resemblance (29% identity) to tetrameric inositol monophosphatase from Thermotoga maritima. The protein was cloned, expressed, purified to homogeneity and crystallized. Crystals appeared in several conditions and good diffraction-quality crystals were obtained from 0.2 M Li(2)SO(4), 20% PEG 3350, 0.1 M HEPES pH 7.0 using the sitting-drop vapour-diffusion method. A complete diffraction data set was collected to 2.6 Å resolution using a Rigaku MicroMax-007 HF Cu Kα X-ray generator and a Rigaku R-AXIS IV(++) detector. The diffraction data were consistent with the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 49.98, b = 68.35, c = 143.79 Å, α = β = γ = 90°, and the crystal contained two molecules in the asymmetric unit.
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur, West Bengal 721 302, India.
authors.forename: Sudipta
authors.initials: S
authors.lastname: Bhattacharyya
authors.forename: Debajyoti
authors.initials: D
authors.lastname: Dutta
authors.forename: Ananta Kumar
authors.initials: AK
authors.lastname: Ghosh
authors.forename: Amit Kumar
authors.initials: AK
authors.lastname: Das
mesh_terms: D003001:Cloning, Molecular; D018360:Crystallography, X-Ray; D010744:Phosphoric Monoester Hydrolases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D013211:Staphylococcus aureus / Q000201:enzymology*
chemical_list: D010744:Phosphoric Monoester Hydrolases; C021822:myo-inositol-1 (or 4)-monophosphatase