title: [The expression and clinical significance of RABEX-5 and RAB5 in breast cancer].
abstract: OBJECTIVE
  To study the expression and significance of RABEX-5 and RAB5 in human breast cancer.
  
  
  METHODS
  In this study the expression of RABEX-5 and RAB5 mRNA and protein was detected by Immunohistochemical techniques, the reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot assay in 60 cases of breast cancer, 15 cases of breast fibroadenomas and 15 cases of normal breast tissue. The relationship of RABEX-5, RAB5 and the clinic pathological features was also analyzed.
  
  
  RESULTS
  Expression of RABEX-5 and RAB5 in breast cancer was obviously higher than that in breast fibroadenomas and normal breast tissue (P < 0.05). RABEX-5 and RAB5 expression level in tumor diameter (T) >5 cm group of human breast cancer was obviously higher than that in T < or =2 cm and 2 < T < or =5 cm group (P < 0.05). RABEX-5 and RAB5 expression level in axillary lymph node positive group was obviously higher than that in negative group (P < 0.05). However, there were no significant differences of RABEX-5 and RAB5 levels between HER-2 (+) group and HER-2 (-) group (P > 0.05), ER (-) group and ER (+) group (P > 0.05), PR (-) group and PR (+) group (P> 0.05), and in histological grade I, II and III, there also were no significant differences of RABEX-5 and RAB5 levels. The expression of RABEX-5 is correlated with RAB5 in both mRNA and protein level.
  
  
  CONCLUSIONS
  RABEX-5 and RAB5 may be involved in the development of breast cancer metastasis.
authors.affiliation: Department of Endorcrine Surgery, the First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Ying-jian
authors.initials: YJ
authors.lastname: Wang
authors.forename: Hong-yuan
authors.initials: HY
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Jie
authors.initials: J
authors.lastname: Min
mesh_terms: D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000378:metabolism*; D005260:Female; D020662:Guanine Nucleotide Exchange Factors / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008875:Middle Aged; D009362:Neoplasm Metastasis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020696:rab5 GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D020662:Guanine Nucleotide Exchange Factors; C108382:RABGEF1 protein, human; D012333:RNA, Messenger; D020696:rab5 GTP-Binding Proteins