title: Speciation of arsenic in urine following intravenous administration of arsthinol in mice.
abstract: Recent investigations have shown that arsthinol, a trivalent organoarsenic compound (dithiarsolane), has been active in vitro on leukemia cell lines and offers a better therapeutic index than arsenic trioxide, as estimated by the ratio LD50/IC50. To complete our understanding of its urinary excretion, a sensitive method using liquid chromatography coupled with mass spectrometry (LC-MS) was used. Mice were injected intravenously with a single dose of arsthinol at 0.2 mmol/kg of body weight. The amount of total arsenic in tissues and body fluids was determined by a colorimetric method and urine metabolites were analyzed on a C18 Acclaim PepMap 100 A column by LC-MS. Our results showed that only three arsenic species (acetarsol, acetarsol oxide and arsthinol) were detected in the first 24-h urine. Overall, this study confirms that the hydrolysis of dithiarsolanes to arsenoxides (i.e. acetarsol oxide) can be followed by an oxidation in arsonic acids (i.e. acetarsol). All these compounds are excreted in the urine.
authors.affiliation: Pharmacie Clinique, EA 3452 Cibles therapeutiques, formulation et expertise pré-clinique du médicament", Université Henri Poincaré, Nancy Université, 5, rue Albert Lebrun, BP 80403, 54001 Nancy Cedex, France.
authors.forename: Imane
authors.initials: I
authors.lastname: Ajana
authors.forename: Alain
authors.initials: A
authors.lastname: Astier
authors.forename: Stéphane
authors.initials: S
authors.lastname: Gibaud
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000493:pharmacokinetics* / Q000652:urine; D001151:Arsenic / Q000493:pharmacokinetics*; D001152:Arsenicals / Q000493:pharmacokinetics* / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D005260:Female; D013058:Mass Spectrometry; D051379:Mice; D010087:Oxides / Q000652:urine; D014556:Urine / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D001152:Arsenicals; D010087:Oxides; C005284:acetarsol; D001151:Arsenic; C546471:arsthinol