title: Differential expression of a set of genes in follicular and classic variants of papillary thyroid carcinoma.
abstract: Fine-needle aspiration biopsy (FNA) is currently the best initial diagnostic test for evaluation of a thyroid nodule. FNA cytology cannot discriminate between benign and malignant thyroid nodules in up to 30% of thyroid nodules. Therefore, an adjunct to FNA is needed to clarify these lesions as benign or malignant. Using differential display-polymerase chain reaction method, the gene expression differences between follicular and classic variants of papillary thyroid carcinoma (PTC) and benign thyroid nodules were evaluated in a group of 42 patients. Computational gene function analyses via Cytoscape, FuncBASE, and GeneMANIA led us to a functional network of 17 genes in which a core sub-network of five genes coexists. Although the exact mechanisms underlying in thyroid cancer biogenesis are not currently known, our data suggest that the pattern of transformation from healthy cells to cancer cells of PTC is different in follicular variant than in classic variant.
authors.affiliation: Department of Medical Biology, Faculty of Medicine, University of Gaziantep, 27310, Gaziantep, Turkey. igci@gantep.edu.tr
authors.forename: Yusuf Ziya
authors.initials: YZ
authors.lastname: Igci
authors.forename: Ahmet
authors.initials: A
authors.lastname: Arslan
authors.forename: Ersin
authors.initials: E
authors.lastname: Akarsu
authors.forename: Suna
authors.initials: S
authors.lastname: Erkilic
authors.forename: Mehri
authors.initials: M
authors.lastname: Igci
authors.forename: Serdar
authors.initials: S
authors.lastname: Oztuzcu
authors.forename: Beyhan
authors.initials: B
authors.lastname: Cengiz
authors.forename: Bulent
authors.initials: B
authors.lastname: Gogebakan
authors.forename: Ecir Ali
authors.initials: EA
authors.lastname: Cakmak
authors.forename: A Tuncay
authors.initials: AT
authors.lastname: Demiryurek
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D044963:Biopsy, Fine-Needle; D002277:Carcinoma; D002291:Carcinoma, Papillary / Q000175:diagnosis* / Q000235:genetics; D018265:Carcinoma, Papillary, Follicular / Q000175:diagnosis* / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000077273:Thyroid Cancer, Papillary; D013964:Thyroid Neoplasms / Q000175:diagnosis* / Q000235:genetics; D016606:Thyroid Nodule / Q000175:diagnosis* / Q000235:genetics; D055815:Young Adult