title: [Optimization of extraction procedure of tongmai granules by orthogonal design with pharmacodynamic index].
abstract: OBJECTIVE
  To optimize the extraction technology for anti-myocardial ischemia active component group in tongmai granules.
  
  
  METHODS
  The extracting conditions were optimized by using orthogonal experiment and pharmacodynamic index. Employing puerarin, ferulic acid, tanshinone IIA, and salvianolic acid B as indexes, the effects of ethanol concentration, solvent volume, extraction times and extraction time on the extracting process were investigated.
  
  
  RESULTS
  The optimum extraction process was adding 8 times amount of 70% alcohol, refluxing and extracting for 3 times, 1.5 h each time.
  
  
  CONCLUSIONS
  This extraction process shows good stability and is available for extracting anti-myocardial ischemia component group in tongmnai granules.
authors.affiliation: School of Pharmacy, Jiangsu University, Zhenjiang 212013, China. jxiaobin2005@hotmail.com
authors.forename: Xiaobin
authors.initials: X
authors.lastname: Jia
authors.forename: Yang
authors.initials: Y
authors.lastname: Huang
authors.forename: Bin
authors.initials: B
authors.lastname: Chen
authors.forename: Qi
authors.initials: Q
authors.lastname: Liu
authors.forename: Zhongwen
authors.initials: Z
authors.lastname: Sun
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000302:isolation & purification* / Q000493:pharmacokinetics*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013678:Technology, Pharmaceutical / Q000295:instrumentation / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal