title: A mouse reporter line to conditionally mark nuclei and cell membranes for in vivo live-imaging.
abstract: Live-imaging is an essential tool to visualize live cells and monitor their behaviors during development. This technology demands a variety of mouse reporter lines, each uniquely expressing a fluorescent protein. Here, we developed an R26R-RG reporter mouse line that conditionally and simultaneously expresses mCherry and EGFP in nuclei and plasma membranes, respectively, from the Rosa26 locus. The intensity and resolution of mCherry nuclear localization and EGFP membrane localization were demonstrated to be sufficient for live-imaging with embryos that express RG (mCherry and EGFP) ubiquitously and specifically in fetal Sertoli cells. The conditional R26R-RG reporter mouse line should be a useful tool for labeling nuclei and membranes simultaneously in distinct cell populations.
authors.affiliation: Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Developmental Biology (CDB), Kobe 650-0047, Japan.
authors.forename: Go
authors.initials: G
authors.lastname: Shioi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kiyonari
authors.forename: Takaya
authors.initials: T
authors.lastname: Abe
authors.forename: Kazuki
authors.initials: K
authors.lastname: Nakao
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Fujimori
authors.forename: Chuan-Wei
authors.initials: CW
authors.lastname: Jang
authors.forename: Cheng-Chiu
authors.initials: CC
authors.lastname: Huang
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Akiyama
authors.forename: Richard R
authors.initials: RR
authors.lastname: Behringer
authors.forename: Shinichi
authors.initials: S
authors.lastname: Aizawa
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000235:genetics / Q000378:metabolism*; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism*; D047108:Embryonic Development / Q000235:genetics; D005260:Female; D018507:Gene Expression Regulation, Developmental; D023061:Gene Order; D018390:Gene Targeting; D056426:Genetic Loci; D005838:Genotype; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D057054:Molecular Imaging; D009928:Organ Specificity / Q000235:genetics; D013194:Staining and Labeling; D059008:Time-Lapse Imaging