title: VEGF-A-induced immature DCs not mature DCs differentiation into endothelial-like cells through ERK1/2-dependent pathway.
abstract: Dendritic cells (DCs) are professional antigen-presenting cells that play an important role in anti-tumour immunity. Endothelial-like differentiation of DCs is an interesting phenomenon. The specific role of vascular endothelial growth factor-A (VEGF-A) on the differentiation of immature DCs (iDCs) and mature DCs (mDCs) is worth further research. Here, we show that VEGF-A can induce iDCs to differentiate into endothelial-like cells (ELCs). But it has no obvious influence on mDCs. In the process of endothelial-like differentiation of iDCs, a sustained activation of extracellular signal-regulated kinase (ERK1/2) and cAMP response element binding protein (CREB) was detected. VEGF-A induced the activation of ERK1/2, and led to the nuclear translocation of phosphorylation ERK1/2. Incubation of iDCs with the ERK1/2 upstream kinase MEK1/2 inhibitor PD98059, blocked the phosphorylation of ERK1/2 and CREB as well as the endothelial-like differentiation of iDCs. These data suggest that VEGF-A induces endothelial-like differentiation of iDCs not mDCs through ERK1/2 signalling pathway.
authors.affiliation: Department of Pathophysiology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Lu
authors.forename: Jun
authors.initials: J
authors.lastname: Zhao
authors.forename: Jimin
authors.initials: J
authors.lastname: Zhao
authors.forename: Junfen
authors.initials: J
authors.lastname: Ma
authors.forename: Kangdong
authors.initials: K
authors.lastname: Liu
authors.forename: Hongyan
authors.initials: H
authors.lastname: Yang
authors.forename: Youtian
authors.initials: Y
authors.lastname: Huang
authors.forename: Zhenzhu
authors.initials: Z
authors.lastname: Qin
authors.forename: Ruihua
authors.initials: R
authors.lastname: Bai
authors.forename: Pei
authors.initials: P
authors.lastname: Li
authors.forename: Wenhai
authors.initials: W
authors.lastname: Yan
authors.forename: Mingyao
authors.initials: M
authors.lastname: Zhao
authors.forename: Ziming
authors.initials: Z
authors.lastname: Dong
mesh_terms: D021581:Active Transport, Cell Nucleus; D002454:Cell Differentiation; D002478:Cells, Cultured; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism*; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects; D005419:Flavonoids / Q000494:pharmacology; D005434:Flow Cytometry; D006801:Humans; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism*; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism*; D010766:Phosphorylation; D015398:Signal Transduction; D014471:Umbilical Veins / Q000166:cytology; D042461:Vascular Endothelial Growth Factor A / Q000494:pharmacology*
chemical_list: D017362:Cyclic AMP Response Element-Binding Protein; D005419:Flavonoids; D042461:Vascular Endothelial Growth Factor A; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one