title: 8-aminoquinoline functionalized silica nanoparticles: a fluorescent nanosensor for detection of divalent zinc in aqueous and in yeast cell suspension.
abstract: Zinc is one of the most important transition metal of physiological importance, existing primarily as a divalent cation. A number of sensors have been developed for Zn(II) detection. Here, we present a novel fluorescent nanosensor for Zn(II) detection using a derivative of 8-aminoquinoline (N-(quinolin-8-yl)-2-(3 (triethoxysilyl)propylamino)acetamide (QTEPA) grafted on silica nanoparticles (SiNPs). These functionalized SiNPs were used to demonstrate specific detection of Zn(II) in tris-HCl buffer (pH 7.22), in yeast cell (Saccharomyces cerevisiae) suspension, and in tap water. The silane QTEPA, SiNPs and final product were characterized using solution and solid state nuclear magnetic resonance, Fourier transform infrared, ultraviolet-visible absorption spectroscopy, transmission electron microscopy, elemental analysis, thermogravimetric techniques, and fluorescence spectroscopy. The nanosensor shows almost 2.8-fold fluorescence emission enhancement and about 55 nm red-shift upon excitation with 330 ± 5 nm wavelength in presence of 1 μM Zn(II) ions in tris-HCl (pH 7.22). The presence of other metal ions has no observable effect on the sensitivity and selectivity of nanosensor. This sensor selectively detects Zn(II) ions with submicromolar detection to a limit of 0.1 μM. The sensor shows good applicability in the determination of Zn(II) in tris-HCl buffer and yeast cell environment. Further, it shows enhancement in fluorescence intensity in tap water samples.
authors.affiliation: Department of Chemistry, University of Idaho, Moscow, Idaho 83844-2343, USA. srastogi@uidaho.edu
authors.forename: Shiva K
authors.initials: SK
authors.lastname: Rastogi
authors.forename: Parul
authors.initials: P
authors.lastname: Pal
authors.forename: D Eric
authors.initials: DE
authors.lastname: Aston
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Bitterwolf
authors.forename: A Larry
authors.initials: AL
authors.lastname: Branen
mesh_terms: D000634:Aminoquinolines / Q000737:chemistry / Q000378:metabolism*; D002413:Cations, Divalent / Q000032:analysis; D005453:Fluorescence; D005456:Fluorescent Dyes; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000737:chemistry*; D013535:Suspensions / Q000737:chemistry; D014867:Water / Q000737:chemistry*; D015032:Zinc / Q000032:analysis*
chemical_list: D000634:Aminoquinolines; D002413:Cations, Divalent; D005456:Fluorescent Dyes; D013535:Suspensions; D014867:Water; D012822:Silicon Dioxide; D015032:Zinc; C080436:8-aminoquinoline