title: Respiratory distress and perinatal lethality in Nedd4-2-deficient mice.
abstract: The epithelial sodium channel (ENaC) is essential for sodium homoeostasis in many epithelia. ENaC activity is required for lung fluid clearance in newborn animals and for maintenance of blood volume and blood pressure in adults. In vitro studies show that the ubiquitin ligase Nedd4-2 ubiquitinates ENaC to regulate its cell surface expression. Here we show that knockout of Nedd4-2 in mice leads to increased ENaC expression and activity in embryonic lung. This increased ENaC activity is the likely reason for premature fetal lung fluid clearance in Nedd4-2(-/-) animals, resulting in a failure to inflate lungs and perinatal lethality. A small percentage of Nedd4-2(-/-) animals survive up to 22 days, and these animals also show increased ENaC expression and develop lethal sterile inflammation of the lung. Thus, we provide critical in vivo evidence that Nedd4-2 is essential for correct regulation of ENaC expression, fetal and postnatal lung function and animal survival.
authors.affiliation: Department of Haematology, Centre for Cancer Biology, SA Pathology, PO Box 14, Rundle Mall, Adelaide, South Australia 5000, Australia.
authors.forename: Natasha A
authors.initials: NA
authors.lastname: Boase
authors.forename: Grigori Y
authors.initials: GY
authors.lastname: Rychkov
authors.forename: Scott L
authors.initials: SL
authors.lastname: Townley
authors.forename: Anuwat
authors.initials: A
authors.lastname: Dinudom
authors.forename: Eleanora
authors.initials: E
authors.lastname: Candi
authors.forename: Anne K
authors.initials: AK
authors.lastname: Voss
authors.forename: Tatiana
authors.initials: T
authors.lastname: Tsoutsman
authors.forename: Chris
authors.initials: C
authors.lastname: Semsarian
authors.forename: Gerry
authors.initials: G
authors.lastname: Melino
authors.forename: Frank
authors.initials: F
authors.lastname: Koentgen
authors.forename: David I
authors.initials: DI
authors.lastname: Cook
authors.forename: Sharad
authors.initials: S
authors.lastname: Kumar
mesh_terms: D000818:Animals; D001794:Blood Pressure; D015152:Blotting, Northern; D018893:Bronchoalveolar Lavage; D018925:Chemokines / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D004622:Embryo, Mammalian / Q000378:metabolism / Q000473:pathology; D056827:Endosomal Sorting Complexes Required for Transport / Q000172:deficiency* / Q000378:metabolism; D053503:Epithelial Sodium Channels / Q000378:metabolism*; D015151:Immunoblotting; D008168:Lung / Q000196:embryology / Q000378:metabolism / Q000473:pathology*; D008171:Lung Diseases / Q000235:genetics* / Q000401:mortality; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D000075702:Nedd4 Ubiquitin Protein Ligases; D044767:Ubiquitin-Protein Ligases / Q000172:deficiency* / Q000378:metabolism
chemical_list: D018925:Chemokines; D016207:Cytokines; D056827:Endosomal Sorting Complexes Required for Transport; D053503:Epithelial Sodium Channels; D000075702:Nedd4 Ubiquitin Protein Ligases; C000618461:Nedd4l protein, mouse; D044767:Ubiquitin-Protein Ligases