title: Antifibrotic constituents of Alnus firma on hepatic stellate cells.
abstract: Suppression of hepatic stellate cell (HSC) growth and activation have been proposed as therapeutic strategies for the treatment and prevention of liver fibrosis. In the course of screening antifibrotic activity of natural products, the methanolic extract of Alnus firma barks (Betulaceae) showed inhibitory activity of cell proliferation on HSC-T6 cells. A new triterpenoid characterized as lup-20(29) en-2,28-diol-3-yl caffeate (13) was isolated with 12 known diarylheptanoids (1-12) from the barks of A. firma using bioactivity-guided fractionation. Among these compounds, 2 and 13 significantly inhibited the proliferation of HSCs in dose- and time-dependent manners at concentrations from 10 to 100 μM. Taken together, antifibrotic activities of A. firma and its active constituents might suggest the therapeutic potentials against liver fibrosis.
authors.affiliation: College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Mina
authors.initials: M
authors.lastname: Lee
authors.forename: Mi Kyeong
authors.initials: MK
authors.lastname: Lee
authors.forename: Young Choong
authors.initials: YC
authors.lastname: Kim
authors.forename: Sang Hyun
authors.initials: SH
authors.lastname: Sung
mesh_terms: D029661:Alnus / Q000737:chemistry*; D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D036381:Diarylheptanoids / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D055166:Hepatic Stellate Cells / Q000187:drug effects*; D020128:Inhibitory Concentration 50; D008103:Liver Cirrhosis / Q000188:drug therapy; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D024301:Plant Bark / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats
chemical_list: D036381:Diarylheptanoids; D010936:Plant Extracts