title: Anti-dermatitis effects of oak wood vinegar on the DNCB-induced contact hypersensitivity via STAT3 suppression.
abstract: OBJECTIVE
  The aim of this study was to evaluate the anti-dermatitis effects of oak wood vinegar (OWV) in a 2,4-dinitrochlorobenzene (DNCB)-induced contact dermatitis mice model.
  
  
  METHODS
  Immunoglobulin E (IgE) production, infiltration of immune cells (neutrophils, CD3+ cells), inducible nitric oxide synthase (iNOS) expression, skin thickness, and expression of phosphorylated STAT3 (signal transducers and activators of transcription 3) protein were tested in a DNCB-induced contact dermatitis model. In vitro wound healing and proliferative assays were also performed.
  
  
  RESULTS
  OWV showed anti-inflammatory effects on DNCB-induced dermatitis in mice, leading to inhibition of IgE production, immune cell infiltration, and iNOS expression. Skin thickness and the level of phospho-STAT3 were dramatically reduced by OWV. Using the HaCaT human keratinocyte cell line, we confirmed that constitutive STAT3 activation induced faster proliferation of epithelial cells. In addition, OWV suppressed HaCaT proliferative ability and phospho-STAT3 levels.
  
  
  CONCLUSIONS
  The study revealed that OWV has anti-inflammatory and anti-proliferative effects in a DNCB-induced contact dermatitis mice model. Furthermore, we showed that the mechanism by which OWV most likely inhibits epithelial proliferation is through STAT3 inactivation.
authors.affiliation: Department of Pharmacology and Ischemic/Hypoxic Disease Institute, Seoul National University College of Medicine, 103 Daehangno, Jongno-gu, Seoul 110-799, Republic of Korea.
authors.forename: Chang Seok
authors.initials: CS
authors.lastname: Lee
authors.forename: Eun Hee
authors.initials: EH
authors.lastname: Yi
authors.forename: Hang-Rae
authors.initials: HR
authors.lastname: Kim
authors.forename: Sang-Rok
authors.initials: SR
authors.lastname: Huh
authors.forename: Sang-Hyun
authors.initials: SH
authors.lastname: Sung
authors.forename: Myung-Hee
authors.initials: MH
authors.lastname: Chung
authors.forename: Sang-Kyu
authors.initials: SK
authors.lastname: Ye
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D017449:Dermatitis, Allergic Contact / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D004137:Dinitrochlorobenzene; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D055633:Immune System Phenomena / Q000187:drug effects; D007073:Immunoglobulin E / Q000096:biosynthesis; D015603:Keratinocytes / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D008517:Phytotherapy; D028321:Plant Preparations / Q000494:pharmacology / Q000627:therapeutic use*; D029963:Quercus; D050796:STAT3 Transcription Factor / Q000037:antagonists & inhibitors*; D012867:Skin / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D014934:Wood
chemical_list: D000893:Anti-Inflammatory Agents; D004137:Dinitrochlorobenzene; D028321:Plant Preparations; D050796:STAT3 Transcription Factor; D007073:Immunoglobulin E; D052247:Nitric Oxide Synthase Type II