title: Circulating tissue factor and microparticles are not increased in patients with deep vein thrombosis.
abstract: BACKGROUND
  Circulating tissue factor (TF) is associated with inflammation and may contribute to thrombotic events. Aim of this study was to analyze circulating TF activity and proinflammatory cytokines in patients with deep venous thrombosis.
  
  
  METHODS
  Forty-eight patients with deep vein thrombosis and 45 control subjects were included. Venous blood samples were obtained at diagnosis for analysis of TF activity, TF antigen, prothrombin fragment F1 + 2, microparticles (expressing phosphatidylserine and supporting FXa generation), Interleukin (IL)-1beta, IL-6, IL-8, IL-10, IL-12 and tumor-necrosis-factor-alpha (TNF).
  
  
  RESULTS
  TF antigen, activity and microparticles were similar in both groups: In contrast, a significant increase in plasma IL-6, IL-8 and F1 + 2 levels was found in thrombosis. This increase in IL-6 and IL-8 as well as F1 + 2 was not correlated with the extent of thrombosis, predisposing factors or onset of symptoms.
  
  
  CONCLUSIONS
  Circulating TF and microparticles are not elevated in deep venous thrombosis. The increase in IL-6, IL-8 and F1 + 2 during thrombosis was not proportional to the extent or predisposing risk factors.
authors.affiliation: 1. Medizinische Klinik, Klinikums rechts der Isar der Technischen Universität München, München, Germany.
authors.forename: B A
authors.initials: BA
authors.lastname: Steppich
authors.forename: M
authors.initials: M
authors.lastname: Hassenpflug
authors.forename: S L
authors.initials: SL
authors.lastname: Braun
authors.forename: K
authors.initials: K
authors.lastname: Schömig
authors.forename: O
authors.initials: O
authors.lastname: von Beckerath
authors.forename: N
authors.initials: N
authors.lastname: von Beckerath
authors.forename: H-H
authors.initials: HH
authors.lastname: Eckstein
authors.forename: I
authors.initials: I
authors.lastname: Ott
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D055252:Cell-Derived Microparticles / Q000473:pathology*; D016207:Cytokines / Q000097:blood; D005260:Female; D005858:Germany; D006801:Humans; D018836:Inflammation Mediators / Q000097:blood; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D018570:Risk Assessment; D012307:Risk Factors; D013925:Thromboplastin / Q000032:analysis*; D020246:Venous Thrombosis / Q000097:blood* / Q000209:etiology / Q000473:pathology
chemical_list: D015415:Biomarkers; D016207:Cytokines; D018836:Inflammation Mediators; D013925:Thromboplastin