title: Evaluation of Turkish seaweeds for antiprotozoal, antimycobacterial and cytotoxic activities.
abstract: As part of our continuing research on seaweeds, crude MeOH extracts of two green, three brown and six red algae collected from Marmara, Black, Aegean and Mediterranean Seas were screened. Four parasitic protozoa, i.e. Plasmodium falciparum, Trypanosoma brucei rhodesiense, T. cruzi, Leishmania donovani and the tubercle bacillus Mycobacterium tuberculosis were used as test organisms for the in vitro assays. The selective toxicity of the extracts was also determined against mammalian L6 cells. All seaweed extracts were active against T. brucei rhodesiense; the Dasya pedicellata extract was the most potent (IC(50) value 0.37 µg/mL). The same extract also weakly inhibited the growth of T. cruzi (IC(50) 62.02 µg/mL). All seaweed extracts also showed leishmanicidal activity (IC(50) values 16.76-69.98 µg/mL). The majority of the extracts also exhibited antiplasmodial potential and the most potent extracts were those from D. pedicellata (IC(50) 0.38 µg/mL), Codium bursa (IC(50) 1.38 µg/mL) and Caulerpa rasemosa (IC(50) 3.12 µg/mL). One brown and two red algal extracts showed some weak activity against Mycobacterium tuberculosis (MIC values 125-256 µg/mL). Except for the extract of Dasya pedicellata, none of the extracts displayed any cytotoxicity. This is the second study investigating the antiprotozoal activities of Turkish marine algae and identifies Dasya pedicellata, an understudied algal species, as a candidate for further studies.
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, University of Istanbul, Istanbul, Turkey.
authors.forename: Sevda
authors.initials: S
authors.lastname: Süzgeç-Selçuk
authors.forename: Ali H
authors.initials: AH
authors.lastname: Meriçli
authors.forename: Kasım C
authors.initials: KC
authors.lastname: Güven
authors.forename: Marcel
authors.initials: M
authors.lastname: Kaiser
authors.forename: Rosalyn
authors.initials: R
authors.lastname: Casey
authors.forename: Suzie
authors.initials: S
authors.lastname: Hingley-Wilson
authors.forename: Ajit
authors.initials: A
authors.lastname: Lalvani
authors.forename: Deniz
authors.initials: D
authors.lastname: Tasdemir
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000981:Antiprotozoal Agents / Q000494:pharmacology*; D059038:Black Sea; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D000460:Chlorophyta / Q000737:chemistry; D003603:Cytotoxins / Q000494:pharmacology*; D020128:Inhibitory Concentration 50; D007893:Leishmania donovani / Q000187:drug effects; D008522:Mediterranean Sea; D008826:Microbial Sensitivity Tests; D009169:Mycobacterium tuberculosis / Q000187:drug effects; D021261:Parasitic Sensitivity Tests; D000459:Phaeophyceae / Q000737:chemistry; D010963:Plasmodium falciparum / Q000187:drug effects; D051381:Rats; D000461:Rhodophyta / Q000737:chemistry; D012624:Seaweed / Q000737:chemistry*; D014345:Trypanosoma / Q000187:drug effects; D014421:Turkey
chemical_list: D000900:Anti-Bacterial Agents; D000981:Antiprotozoal Agents; D003603:Cytotoxins