title: Immunodeficient NOD-scid IL-2Rγ(null) mice do not display T and B cell leakiness.
abstract: NOD/Shi-scid IL-2Rγ(null) (NOG) mice established by introducing the IL-2Rγ(null) gene of IL-2Rγ KO mice into NOD/Shi-scid mice by backcross-mating show a high xenograft engraftment level and are therefore well suited as a humanized mouse model. SCID mice bearing the Prkdc(scid) gene show a high incidence of thymic lymphoma and a leaky phenomenon in which a few clonal T and B cells develop in aged mice. In the present study, NOG mice were assessed for the presence of a leaky phenomenon such as the one observed in C.B-17-scid and NOD-scid mice. Serum immunoglobulin analysis did not detect IgG or IgM in NOG mice, unlike the findings in C.B-17-scid and NOD-scid mice. Flow cytometry analysis revealed the absence of T and B cells in the peripheral blood and spleens of NOG mice. These results reflect the suppression of the leaky phenomenon in NOG mice through the inactivation of the IL-2Rγ gene, which is commonly expressed in T and B cell growth factor receptors to IL-2, IL-4 and IL-7.
authors.affiliation: Central Institute for Experimental Animals, 1430 Nogawa, Kawasaki 216-0001, Japan.
authors.forename: Ikumi
authors.initials: I
authors.lastname: Katano
authors.forename: Ryoji
authors.initials: R
authors.lastname: Ito
authors.forename: Tomoo
authors.initials: T
authors.lastname: Eto
authors.forename: Sadakazu
authors.initials: S
authors.lastname: Aiso
authors.forename: Mamoru
authors.initials: M
authors.lastname: Ito
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology* / Q000473:pathology; D003433:Crosses, Genetic; D004195:Disease Models, Animal; D005434:Flow Cytometry; D007136:Immunoglobulins / Q000097:blood*; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D013154:Spleen / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology* / Q000473:pathology
chemical_list: D007136:Immunoglobulins