title: NaOH-induced Crohn's colitis in rats: a novel experimental model.
abstract: OBJECTIVE
  Several models of experimental ulcerative colitis have been previously reported. To date, only one model of Crohn's colitis that was induced by trinitrobenzene sulfonic acid has been described.
  
  
  METHODS
  Twenty-one male albino rats were divided into three groups: group I: sham control group (n = 7), group II: acetic acid group (n = 7), group III: NaOH group (n = 7). On the day of induction, all rats were lightly anesthetized with intramuscular ketamine (8 mg/kg). A 6F plastic catheter was inserted rectally until the tip was 5 cm proximal to the anus. Then, 2 ml of 0.9% saline, 2 ml of 4% acetic acid, and 2 ml of 6.25% NaOH was administered to groups I, II, and III, respectively. All rats were sacrificed 5 days after colitis induction. The distal colon segment was assessed macroscopically and microscopically. In addition, malondialdehyde (MDA) and nitric oxide (NO) levels of the colonic tissue and changes in body weight were measured.
  
  
  RESULTS
  Macroscopic and microscopic examinations of colonic tissue samples showed morphological similarities to human Crohn's disease (CD). The MDA and NO levels of the colonic tissues were significantly higher in the NaOH group compared to the acetic acid and sham control groups (P = 0.001).
  
  
  CONCLUSIONS
  NaOH may be used to induce Crohn's colitis as an experimental model.
authors.affiliation: Department of Gastroenterology, Ankara Education and Research Hospital, Keklikpınarı mahallesi, 240 sokak, Duru apartmanı, 8/6, Dikmen, Çankaya, Ankara, Turkey. kocak67@hotmail.com
authors.forename: Erdem
authors.initials: E
authors.lastname: Koçak
authors.forename: Seyfettin
authors.initials: S
authors.lastname: Köklü
authors.forename: Erdem
authors.initials: E
authors.lastname: Akbal
authors.forename: Adnan
authors.initials: A
authors.lastname: Taş
authors.forename: Gökhan
authors.initials: G
authors.lastname: Karaca
authors.forename: Müzeyyen Hesna
authors.initials: MH
authors.lastname: Astarcı
authors.forename: Berrak
authors.initials: B
authors.lastname: Güven
authors.forename: Murat
authors.initials: M
authors.lastname: Can
mesh_terms: D000818:Animals; D001835:Body Weight / Q000502:physiology; D003106:Colon / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D003424:Crohn Disease / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology*; D004195:Disease Models, Animal; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012972:Sodium Hydroxide / Q000009:adverse effects* / Q000494:pharmacology
chemical_list: D009569:Nitric Oxide; D008315:Malondialdehyde; D012972:Sodium Hydroxide