title: Supernatant of bone marrow mesenchymal stromal cells induces peripheral blood mononuclear cells possessing mesenchymal features.
abstract: Increasing evidence shows that some cells from peripheral blood fibroblast-like mononuclear cells have the capacity to differentiate into mesenchymal lineages. However, the insufficiency of these cells in the circulation challenges the cell isolation and subsequently limits the clinical application of these cells. In the present study, the peripheral blood mononuclear cells (pbMNCs) were isolated from wound animals and treated with the supernatant of bone marrow mesenchymal stromal cells (bmMSCs). Results showed these pbMNCs were fibroblast-like, had stromal morphology, were negative for CD34 and CD45, but positive for Vimentin and Collagen I, and had the multipotency to differentiate into adipocytes and osteoblasts. We named these induced peripheral blood-derived mesenchymal stromal cells (ipbMSCs). Skin grafts in combination with ipbMSCs and collagen I were applied for wound healing, and results revealed ipbMSC exhibited similar potency and effectiveness in the promotion of wound healing to the bmMSCs. Hereafter, we speculate that the mixture of growth factors and chemokines secreted by bmMSCs may play an important roles in the induction of the proliferation and mesenchymal differentiation of mononuclear cells. Our results are clinically relevant because it provide a new method for the acquisition of MSCs which can be used as a candidate for the wound repair.
authors.affiliation: Department of Dermatology, Second Hospital of Xi'an Jiaotong University, Xi'an, 710004, China. gang1008@yeah.net
authors.forename: Gang
authors.initials: G
authors.lastname: Hu
authors.forename: Jun-jun
authors.initials: JJ
authors.lastname: Xu
authors.forename: Zhi-hong
authors.initials: ZH
authors.lastname: Deng
authors.forename: Jie
authors.initials: J
authors.lastname: Feng
authors.forename: Yan
authors.initials: Y
authors.lastname: Jin
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism*; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D019070:Cell Lineage; D024042:Collagen Type I / Q000494:pharmacology; D017077:Culture Media, Conditioned; D005260:Female; D007963:Leukocytes, Mononuclear / Q000166:cytology*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008819:Mice, Nude; D017154:Stromal Cells / Q000378:metabolism*; D014945:Wound Healing
chemical_list: D024042:Collagen Type I; D017077:Culture Media, Conditioned