title: Angiogenesis assays.
abstract: The angiogenic process is central in the pathogenesis of various diseases. The in vitro and in vivo monitoring of the neovascular process is essential for the development and evaluation of angiogenesis inhibitors or stimulators. Since no single method exists that can assess angiogenesis in a robust, reliable, and reproducible fashion, researchers often use a combination of assays to circumvent this problem. The experimental details of the most commonly in vitro, ex vivo, and in vivo assays are presented here.
authors.affiliation: Department of Ophthalmology, Angiogenesis Laboratory, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, MA, USA. polakiv@gmail.com
authors.forename: V
authors.initials: V
authors.lastname: Poulaki
mesh_terms: D000818:Animals; D001011:Aorta / Q000502:physiology; D001681:Biological Assay / Q000379:methods*; D049033:Chorioallantoic Membrane / Q000098:blood supply; D018920:Coculture Techniques; D003094:Collagen / Q000378:metabolism; D003315:Cornea / Q000098:blood supply; D004338:Drug Combinations; D042783:Endothelial Cells / Q000166:cytology; D005260:Female; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D007231:Infant, Newborn; D007797:Laminin / Q000378:metabolism; D008297:Male; D051379:Mice; D009389:Neovascularization, Pathologic; D018919:Neovascularization, Physiologic; D011509:Proteoglycans / Q000378:metabolism; D051381:Rats; D012178:Retinopathy of Prematurity / Q000503:physiopathology
chemical_list: D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen