title: Comparative studies on sorting cells from Artemia sinica at different developmental stages for in vitro cell culture.
abstract: Cell growth in primary cell culture of the brine shrimp (Artemia sinica) embryo at 12 and 20 h after rehydration at 25°C was examined comparatively in modified Leibovitz-15 medium. The cells from A. sinica embryo at 12 h after rehydration were dispersed, and the cells disseminated but did not attach to the surface of wells and multiply at 2 d of culture, and 12 d later, the cells were degenerated and dead. The best growth of the brine shrimp cells was obtained from the prenauplii of A. sinica at 20 h after dormant embryo rehydration. The fibroblast-like cells attached to the well surface and multiplied at 15 d after the primary culture was set up. Confluent monolayer was formed at 50 d. The prenauplii cells have been subcultured up to passage 3 and maintained for approximately 200 d. The reasons for cell growth potential at the different developmental stages of Artemia embryo were discussed.
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China.
authors.forename: Guojian
authors.initials: G
authors.lastname: Jiang
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Xu
authors.forename: Yi
authors.initials: Y
authors.lastname: Jing
authors.forename: Ruixin
authors.initials: R
authors.lastname: Wang
authors.forename: Tingjun
authors.initials: T
authors.lastname: Fan
mesh_terms: D000818:Animals; D001156:Artemia / Q000196:embryology*; D018929:Cell Culture Techniques; D002469:Cell Separation; D004625:Embryo, Nonmammalian / Q000166:cytology*