title: Cytoplasmic HDPR1 is involved in regional lymph node metastasis and tumor development via beta-catenin accumulation in esophageal squamous cell carcinoma.
abstract: The aim of this study was to evaluate HDPR1 expression in esophageal squamous cell carcinoma (ESCC) and the relationship between HDPR1 and beta-catenin by immunohistochemical analysis. The clinical relevance of these proteins was also analyzed. Immunohistochemistry was performed on paraffin-embedded tissue specimens from 184 ESCC patients to detect the expression of HDPR1 and beta-catenin. The correlation between the results of immunoexpression and the clinicopathologic features was processed statistically. Increased cytoplasmic and nuclear HDPR1 expression was noted in 100 (54.3%) and 131 (71.2%) of 184 specimens, respectively. Statistical analysis showed significant associations of cytoplasmic HDPR1 with regional lymph node metastasis (p = 0.021) and P-stage (p = 0.004). The increased nuclear staining was only correlated with P-stage (p = 0.047). Significant associations of coexpression of cytoplasmic and nuclear HDPR1 with regional lymph node metastasis (p = 0.015) or P-stage (p = 0.002) were observed. Enhanced cytoplasmic expression of HDPR1 was positively correlated with increased cytoplasmic but not reduced membranous beta-catenin expression (r = 0.239, p = 0.027 and r = 0.126, p = 0.089, respectively). These finding suggested that cytoplasmic HDPR1 protein expression was associated with tumor malignant progression via beta-catenin accumulation. It implicated that cytoplasmic HDPR1 expression may serve as a potential predictive factor for lymph node metastasis and tumor development in ESCC.
authors.affiliation: Institute of Oncologic Pathology, Medical College of Shantou University, Shantou, Guangdong, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Hou
authors.forename: En-Min
authors.initials: EM
authors.lastname: Li
authors.forename: Jin-Hui
authors.initials: JH
authors.lastname: Shen
authors.lastname: Qing-Zhao
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Wu
authors.forename: Xiu-E
authors.initials: XE
authors.lastname: Xu
authors.forename: Jian-Yi
authors.initials: JY
authors.lastname: Wu
authors.lastname: Qiao-Huang
authors.forename: Jian
authors.initials: J
authors.lastname: Shen
authors.forename: Ming-Zhou
authors.initials: MZ
authors.lastname: Guo
authors.forename: Li-Yan
authors.initials: LY
authors.lastname: Xu
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000096:biosynthesis*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D003593:Cytoplasm / Q000378:metabolism; D004938:Esophageal Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D005260:Female; D006801:Humans; D053208:Kaplan-Meier Estimate; D008198:Lymph Nodes / Q000378:metabolism / Q000473:pathology*; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009687:Nuclear Proteins / Q000096:biosynthesis*; D012189:Retrospective Studies; D015996:Survival Rate; D051176:beta Catenin / Q000096:biosynthesis*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C491683:DACT1 protein, human; D009687:Nuclear Proteins; D051176:beta Catenin