title: [Implication of integrin alpha5beta1 in human breast carcinoma apoptosis and drug resistance].
abstract: Doxorubicin-resistant MCF-7Dox line, which is a derivative of the drug-sensitive MCF-7 human breast carcinoma line, differs from the latter by a strongly reduced expression of the alpha2beta1 integrin and a highly increased expression of the alpha5beta1 receptor. Silencing of this integrin in the MCF-7Dox cells by transfection with alpha5-specific siRNA markedly stimulated anoikis and increased sensitivity of the cells to doxorubicin. Alpha5beta1 silencing also leads to significant inhibition of the activity of kinases Akt and Erk2 in MCF-7Dox cells. Our results suggest that integrins alpha5beta1-induced signals, controlling distinct aspects of cell behavior, are conducted through the common signal pathways.
authors.forename: G E
authors.initials: GE
authors.lastname: Morozevich
authors.forename: N I
authors.initials: NI
authors.lastname: Kozlova
authors.forename: N A
authors.initials: NA
authors.lastname: Ushakova
authors.forename: M E
authors.initials: ME
authors.lastname: Preobrazhenskaia
authors.forename: A E
authors.initials: AE
authors.lastname: Berman
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000493:pharmacokinetics*; D017209:Apoptosis / Q000187:drug effects*; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D004317:Doxorubicin / Q000494:pharmacology*; D019008:Drug Resistance, Neoplasm / Q000187:drug effects*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D039081:Integrin alpha5beta1 / Q000235:genetics / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics
chemical_list: D000903:Antibiotics, Antineoplastic; D039081:Integrin alpha5beta1; D009363:Neoplasm Proteins; D004317:Doxorubicin