title: Scutellarin-dependent inhibition of collagen biosynthesis in cultured fibroblasts.
abstract: The effects of the flavonoid compound scutellarin (SCUT) on collagen biosynthesis, prolidase activity, expression of β₁ integrin, insulin-like growth factor-I (IGF-I) receptor and the transcription factor NF-κB were evaluated in human dermal fibroblasts. Confluent fibroblasts were treated with micromolar concentrations (10-30 µM) of SCUT for 24 h. It was found that a SCUT-dependent decrease in collagen biosynthesis was accompanied by an increase in prolidase activity. Since the IGF-I receptor (IGF-IR) is the most potent regulator of both collagen biosynthesis and prolidase activity, and prolidase is regulated by β₁ integrin signalling, the effect of SCUT on IGF-IR and β₁ integrin receptor expressions were evaluated. It was found that the exposure of the cells to SCUT contributed to an increase in IGF-IR and β₁ integrin receptor expressions. This was accompanied by an increase in expression of NF-κB, the known inhibitor of collagen gene expression. These data suggest that the SCUT-dependent decrease of collagen biosynthesis in cultured human skin fibroblasts results from activation of NF-κB, which is responsible for the down-regulation of collagen gene expression.
authors.affiliation: Department of Medicinal Chemistry, Medical University of Białystok, Kilińskiego 1 Street, Białystok 15-089, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Karna
authors.forename: Jolanta
authors.initials: J
authors.lastname: Nazaruk
authors.forename: Łukasz
authors.initials: Ł
authors.lastname: Szoka
authors.forename: Jerzy A
authors.initials: JA
authors.lastname: Pałka
mesh_terms: D047310:Apigenin / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D015153:Blotting, Western; D003094:Collagen / Q000096:biosynthesis* / Q000187:drug effects; D004150:Dipeptidases / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D049919:Erigeron / Q000737:chemistry*; D005347:Fibroblasts / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects*; D005965:Glucuronates / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D019012:Integrin beta1 / Q000378:metabolism; D015394:Molecular Structure; D016328:NF-kappa B / Q000378:metabolism
chemical_list: D005965:Glucuronates; D019012:Integrin beta1; D016328:NF-kappa B; C484876:scutellarin; D007334:Insulin-Like Growth Factor I; D047310:Apigenin; D003094:Collagen; D004150:Dipeptidases; C022449:proline dipeptidase