title: Effect of cavtratin, a caveolin-1 scaffolding domain peptide, on oligodendroglial signaling cascades.
abstract: Caveolin and caveolin containing rafts are involved in the signaling of growth factors in various cell types. Previous reports of our lab indicated a co-localization of caveolin and the high affinity nerve growth factor (NGF) receptor tyrosine kinase A (TrkA). Mutual effects have been observed among which a caveolin-1 knock-down resulted in an impairment of the NGF signaling cascade rather than in an increase of activity as expected from other growth factor reports. On the other hand, an over-expression of caveolin-1 impaired the NGF stimulated activity of p42/44 mitogen activated protein kinases (MAPK). In this study, we used a caveolin-1 scaffolding domain (CSD) peptide (cavtratin) of which an inhibitory effect on growth factor receptors was reported. Our data showed that cavtratin suppresses the NGF-induced phosphorylation of TrkA as well as the activation of MAPK in porcine oligodendrocytes significantly.
authors.affiliation: Max-Planck Institute of Experimental Medicine, RU Neural Regeneration, Hermann-Rein-Strasse 3, 37075 Göttingen, Germany. matthias.schmitz@med.uni-goettingen.de
authors.forename: Matthias
authors.initials: M
authors.lastname: Schmitz
authors.forename: Inga
authors.initials: I
authors.lastname: Zerr
authors.forename: Hans H
authors.initials: HH
authors.lastname: Althaus
mesh_terms: D000818:Animals; D051242:Caveolin 1 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D006801:Humans; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D020932:Nerve Growth Factor / Q000378:metabolism; D009836:Oligodendroglia / Q000166:cytology / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D020917:Receptor, trkA / Q000378:metabolism; D015398:Signal Transduction; D013552:Swine
chemical_list: D051242:Caveolin 1; D010455:Peptides; D020932:Nerve Growth Factor; D020917:Receptor, trkA; D020928:Mitogen-Activated Protein Kinases