title: (n-3) long-chain PUFA differentially affect resistance to Pseudomonas aeruginosa infection of male and female cftr-/- mice.
abstract: The aim of this study was to determine whether oral supplementation with EPA/DHA (10.5 and 5.1% of fat, respectively) could improve the outcome of pulmonary P. aeruginosa infection in cftr(-/-) mice compared with wild-type (Wt) mice similarly treated. Because gender could influence the susceptibility of cftr-deficient mice, results were analyzed by gender. Wt and (-/-) mice were randomized for 6 wk to consume a control or EPA/DHA diet, infected with endotracheal injection of 5 × 10(7) CFU/mouse of P. aeruginosa, and killed 24 h later. Cftr(-/-) mice were more susceptible to infection than were Wt mice; (-/-) males had more neutrophils (P < 0.01) and a higher keratinocyte-derived chemokine (KC) level (P < 0.05), and (-/-) females had greater lung injury and mortality (P < 0.05). Female (-/-) mice were more susceptible than (-/-) males with a higher mortality and lung injury (P < 0.05). The EPA/DHA diet reduced neutrophil numbers and KC and IL-6 levels (P < 0.05) in (-/-) males and reduced mortality rate (P < 0.001), lung permeability, and IL-6 level (P < 0.05) in (-/-) females compared with (-/-) mice fed the control diet. These results were associated with a reduction in the pulmonary bacterial load (P < 0.05), an increase in the EPA/DHA concentration in cell membranes of (-/-) males and females (P < 0.01), and an increased weight gain only in males compared with (-/-) mice fed the control diet (P < 0.01). In conclusion, EPA/DHA improves the host resistance of (-/-) mice, although the beneficial effect differed in males and females.
authors.affiliation: Inserm U995, IFR 114, University Lille Nord de France, Lille 59045, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Tiesset
authors.forename: Henry
authors.initials: H
authors.lastname: Bernard
authors.forename: Nana
authors.initials: N
authors.lastname: Bartke
authors.forename: Christopher
authors.initials: C
authors.lastname: Beermann
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Flachaire
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Desseyn
authors.forename: Frédéric
authors.initials: F
authors.lastname: Gottrand
authors.forename: Marie-Odile
authors.initials: MO
authors.lastname: Husson
mesh_terms: D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D003550:Cystic Fibrosis / Q000150:complications / Q000178:diet therapy*; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000172:deficiency* / Q000235:genetics; D016207:Cytokines / Q000378:metabolism; D019587:Dietary Supplements; D004198:Disease Susceptibility; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage*; D005260:Female; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism; D008168:Lung / Q000503:physiopathology; D008297:Male; D051379:Mice; D057507:Mice, 129 Strain; D008810:Mice, Inbred C57BL; D018181:Mice, Inbred CFTR; D018345:Mice, Knockout; D009894:Opportunistic Infections / Q000517:prevention & control; D018410:Pneumonia, Bacterial / Q000382:microbiology / Q000503:physiopathology / Q000517:prevention & control*; D011552:Pseudomonas Infections / Q000382:microbiology / Q000503:physiopathology / Q000517:prevention & control*; D012737:Sex Factors
chemical_list: D016207:Cytokines; D015525:Fatty Acids, Omega-3; D018836:Inflammation Mediators; D019005:Cystic Fibrosis Transmembrane Conductance Regulator