title: Characterization of metallo-beta-lactamase VIM-27, an A57S mutant of VIM-1 associated with Klebsiella pneumoniae ST147.
abstract: VIM-27 metallo-β-lactamase, an Ala(57) → Ser variant of VIM-1, was identified in three Klebsiella pneumoniae isolates belonging to sequence type 147. bla(VIM-27) was part of a class 1 integron carried by non-self-transferable plasmids. Kinetic parameters and MIC determinations indicated that VIM-27 hydrolyzed most β-lactams, especially imipenem and cefoxitin, less effectively than VIM-1.
authors.affiliation: Department of Microbiology, Medical School, University of Athens, Athens, Greece.
authors.forename: C C
authors.initials: CC
authors.lastname: Papagiannitsis
authors.forename: S D
authors.initials: SD
authors.lastname: Kotsakis
authors.forename: E
authors.initials: E
authors.lastname: Petinaki
authors.forename: A C
authors.initials: AC
authors.lastname: Vatopoulos
authors.forename: E
authors.initials: E
authors.lastname: Tzelepi
authors.forename: V
authors.initials: V
authors.lastname: Miriagou
authors.forename: L S
authors.initials: LS
authors.lastname: Tzouvelekis
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002440:Cefoxitin / Q000494:pharmacology; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D015378:Imipenem / Q000494:pharmacology; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D015394:Molecular Structure; D001618:beta-Lactamases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D002440:Cefoxitin; D015378:Imipenem; C417264:VIM-1 metallo-beta-lactamase; D001618:beta-Lactamases