title: Genetic relatedness of subgingival and buccal Candida dubliniensis isolates in immunocompetent subjects assessed by RAPD-PCR.
abstract: BACKGROUND
  It is recognized that Candida dubliniensis commonly colonizes oral and subgingival sites in immunocompetent subjects with periodontal disease.
  
  
  OBJECTIVE
  Since there are few data available on genetic characterization of C. dubliniensis in periodontal pockets and other oral sites, the aim of this study was to characterize subgingival and mucosal C. dubliniensis isolates recovered from immunocompetent subjects and to assay the genetic similarity of such isolates from both niches in the same patient by random amplified polymorphic DNA (RAPD).
  
  
  METHODS
  C. dubliniensis recovered from subgingival plaque and from buccal cavity samples were studied in 240 immunocompetent non-smoking individuals. Arbitrary amplification was carried out by RAPD-polymerase chain reaction (PCR).
  
  
  RESULTS
  RAPD analysis showed identical genotypes of C. dubliniensis in different sampling sites (buccal cavity and subgingival areas) in eight of 10 patients except for those derived from two participants who presented presumably unrelated isolates.
  
  
  CONCLUSIONS
  On the basis of the findings presented, the origin of the colonization of C. dubliniensis in subgingival biofilm seems to be the buccal cavity in a single patient. Consequently, it may be assumed that most of C. dubliniensis in these sites arise from the endogenous commensal strains.
authors.affiliation: Department of Microbiology, Parasitology and Immunology, School of Medicine, University of Buenos Aires, Buenos Aires, Argentina.
authors.forename: Virginia Marta
authors.initials: VM
authors.lastname: Jewtuchowicz
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Mujica
authors.forename: Maria Celina
authors.initials: MC
authors.lastname: Malzone
authors.forename: Alicia
authors.initials: A
authors.lastname: Cuesta
authors.forename: Maria Lorena
authors.initials: ML
authors.lastname: Nastri
authors.forename: Cristina Adela
authors.initials: CA
authors.lastname: Iovannitti
authors.forename: Alcira Cristina
authors.initials: AC
authors.lastname: Rosa