title: Typing of Brachyspira spp. from rodents, pigs and chickens on Swedish farms.
abstract: The aim of the current study was to look for evidence of possible cross-species transmission of Brachyspira species between rodents and farm animals. To do this, previously collected and characterised Brachyspira isolates from rodents, pigs and chickens on the same farms were analysed by random amplified polymorphic DNA (RAPD). Isolates with similar RAPD banding patterns were further typed by pulsed-field gel electrophoresis (PFGE). Identical isolates of Brachyspira pilosicoli, Brachyspira intermedia, Brachyspira murdochii and Brachyspira innocens from pigs and rodents and of B. murdochii from laying hens and rodents were found, indicating cross-species transmission at farm level. PFGE data from rodent isolates of Brachyspira hyodysenteriae were compared with PFGE data from previously typed field isolates of B. hyodysenteriae from pigs with swine dysentery and isolates from mallards (Anas platyrhynchos). Three of four isolates of B. hyodysenteriae from rodents were similar to porcine field isolates by PFGE. PCR analyses of the plasmid-encoded and potential virulence determinants rfb genes B, A, D and C showed that they were present in isolates of B. hyodysenteriae of porcine, mallard and rodent origin.
authors.affiliation: Department of Clinical Sciences, Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural Sciences, Uppsala, Sweden. annette.backhans@slu.se
authors.forename: A
authors.initials: A
authors.lastname: Backhans
authors.forename: D S
authors.initials: DS
authors.lastname: Jansson
authors.forename: A
authors.initials: A
authors.lastname: Aspán
authors.forename: C
authors.initials: C
authors.lastname: Fellström
mesh_terms: D000818:Animals; D017400:Brachyspira / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D002645:Chickens; D004372:Ducks; D016521:Electrophoresis, Gel, Pulsed-Field / Q000662:veterinary; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology / Q000635:transmission / Q000662:veterinary*; D010802:Phylogeny; D011201:Poultry Diseases / Q000382:microbiology / Q000635:transmission; D019105:Random Amplified Polymorphic DNA Technique; D051381:Rats; D012376:Rodent Diseases / Q000382:microbiology; D034421:Sus scrofa; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology / Q000635:transmission