title: Hydrolysis of mixed monomolecular films of tricaprylin/dilauroylphosphatidylcholine by lipase and phospholipase A₂.
abstract: The purpose of this article was to describe the kinetics of the enzymatic action of one or more enzymes on mixture of substrates organized in 2D structures in order to mimic some situations existing in biological or industrial systems. Hydrolysis of the mixed monomolecular films of tricaprylin/dilauroylphosphatidylcholine (TC8/DiC12PC) by Thermomyces lanuginosus lipase (TLL) and phospholipase A₂ (PLA₂) was studied by measuring the decrease of the surface area and change of the surface potential at barostatic conditions. The decrease of the surface area detects the transition of the substrate into reaction products and their solubilization while the change of the surface potential detects the contribution of dipole moment of the molecules remaining at the interface during the hydrolysis. The kinetic models, describing the interfacial hydrolysis allowed us to estimate the values of the global kinetic constants for TC8 and DiC12PC hydrolysis, respectively. The role of interaction between all participants of the catalytic act in that complex catalytic system is shown. The catalytic activity of TLL and PLA₂ is affected by the molecular environment in TC8/DiC12PC mixed monolayers.
authors.affiliation: Biophysical Chemistry Laboratory, Department of Physical Chemistry, University of Sofia, Sofia, Bulgaria.
authors.forename: K
authors.initials: K
authors.lastname: Mircheva
authors.forename: Tz
authors.initials: T
authors.lastname: Ivanova
authors.forename: I
authors.initials: I
authors.lastname: Panaiotov
authors.forename: R
authors.initials: R
authors.lastname: Verger
mesh_terms: D002210:Caprylates / Q000737:chemistry* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D008049:Lipase / Q000378:metabolism*; D008567:Membranes, Artificial; D008962:Models, Theoretical; D010713:Phosphatidylcholines / Q000737:chemistry* / Q000378:metabolism*; D054467:Phospholipases A2 / Q000378:metabolism*; D014280:Triglycerides / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D002210:Caprylates; D008567:Membranes, Artificial; D010713:Phosphatidylcholines; D014280:Triglycerides; C015153:1,2-dilauroylphosphatidylcholine; C003637:tricaprylin; D008049:Lipase; D054467:Phospholipases A2