title: Methods for the determination of antioxidant capacity in food and raw materials.
abstract: A comprehensive description of the most frequently used methods to determine the antioxidant activity in food and raw materials is given. The methods are classified into two categories, depending on the type of the assessment carried out. Several methods for the assessment ofantioxidant efficacy using free radical scavenging such as oxygen radical absorbance capacity assay (ORAC), total radical trapping antioxidant parameter assay (TEAC), ferric reducing antioxidant power assay (FRAP) and 2,2'-diphenyl-11-picrylhydrazyl (DPPH) assay are described. An example of methods based on the assessment of antioxidant efficacy using significant biological substrates is also presented. Critical opinions concerning the proposed methods are presented.
authors.affiliation: Centre of Bioanalysis, National Institute for Biological Sciences Bucharest, 296 Splaiul Independentei, Bucharest 6, 060031, Romania. slitescu@gmail.com
authors.forename: Simona Carmen
authors.initials: SC
authors.lastname: Litescu
authors.forename: Sandra
authors.initials: S
authors.lastname: Eremia
authors.forename: Gabriel Lucian
authors.initials: GL
authors.lastname: Radu
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism*; D001681:Biological Assay / Q000379:methods; D001713:Biphenyl Compounds / Q000378:metabolism; D005502:Food; D005609:Free Radicals / Q000378:metabolism; D007202:Indicators and Reagents / Q000378:metabolism; D008954:Models, Biological; D015394:Molecular Structure; D010084:Oxidation-Reduction; D010851:Picrates / Q000378:metabolism; D010944:Plants / Q000737:chemistry*; D017382:Reactive Oxygen Species / Q000737:chemistry / Q000378:metabolism
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D005609:Free Radicals; D007202:Indicators and Reagents; D010851:Picrates; D017382:Reactive Oxygen Species; C004931:1,1-diphenyl-2-picrylhydrazyl