title: Alpha-Type 1 Polarized Dendritic Cells Loaded with Apoptotic Allogeneic Breast Cancer Cells Can Induce Potent Cytotoxic T Lymphocytes against Breast Cancer.
abstract: OBJECTIVE
  Various tumor antigens can be loaded onto dendritic cells (DCs) to induce a potent cytotoxic T lymphocyte (CTL) response in DC-based immunotherapy against breast cancer. However, in the clinical setting, obtaining a sufficient number of autologous tumor cells as a source of tumor antigens is a laborious process. We therefore investigated the feasibility of immunotherapy using breast-cancer-specific CTLs generated in vitro by use of alpha-type 1 polarized DCs (α DC1s) loaded with ultraviolet B-irradiated cells of the breast cancer cell line MCF-7.
  
  
  METHODS
  αDC1s were induced by loading allogeneic tumor antigen generated from the MCF-7 UVB-irradiated breast cancer cell line. Antigen-pulsed αDC1s were evaluated by morphological and functional assays, and the breast-cancer-specific CTL response was analyzed by cytotoxic assay.
  
  
  RESULTS
  The αDC1s significantly increased the expression of several molecules related to DC maturation without differences according to whether the αDC1s were loaded with tumor antigens. The αDC1s showed a high production of interleukin-12 both during maturation and after subsequent stimulation with CD40L, which was not significantly affected by loading with tumor antigens. Breast-cancer-specific CTLs against autologous breast cancer cells were successfully induced by αDC1s loaded with apoptotic MCF-7 cells.
  
  
  CONCLUSIONS
  Autologous DCs loaded with an allogeneic breast cancer cell line can generate potent breast-cancer-specific CTL responses. This may be a practical method for cellular immunotherapy in patients with breast cancer.
authors.affiliation: Department of Surgery, Chonnam National University Hwasun Hospital, Chonnam National University School of Medicine, Hwasun, Korea.
authors.forename: Min-Ho
authors.initials: MH
authors.lastname: Park
authors.forename: Deok-Hwan
authors.initials: DH
authors.lastname: Yang
authors.forename: Mi-Hyun
authors.initials: MH
authors.lastname: Kim
authors.forename: Jae-Hong
authors.initials: JH
authors.lastname: Jang
authors.forename: Yoon-Young
authors.initials: YY
authors.lastname: Jang
authors.forename: Youn-Kyung
authors.initials: YK
authors.lastname: Lee
authors.forename: Chun-Ji
authors.initials: CJ
authors.lastname: Jin
authors.forename: Than Nhan Nguyen
authors.initials: TN
authors.lastname: Pham
authors.forename: Truc Anh Nguyen
authors.initials: TA
authors.lastname: Thi
authors.forename: Mi-Seon
authors.initials: MS
authors.lastname: Lim
authors.forename: Hyun-Ju
authors.initials: HJ
authors.lastname: Lee
authors.forename: Cheol Yi
authors.initials: CY
authors.lastname: Hong
authors.forename: Jung-Han
authors.initials: JH
authors.lastname: Yoon
authors.forename: Je-Jung
authors.initials: JJ
authors.lastname: Lee