title: Nitric oxide content is associated with tolerance to bicarbonate-induced chlorosis in micropropagated Prunus explants.
abstract: Iron (Fe) chlorosis is a common nutritional deficiency in fruit trees grown in calcareous soils. Grafting on tolerant rootstocks is the most efficient practice to cope with it. In the present work, three Prunus hybrid genotypes, commonly used as peach rootstocks, and one peach cultivar were cultivated with bicarbonate in the growth medium. Parameters describing oxidative stress and the metabolism of reactive nitrogen species were studied. Lower contents of nitric oxide and a decreased nitrosoglutathione reductase activity were found in the most sensitive genotypes, characterized by higher oxidative stress and reduced antioxidant defense. In the peach cultivar, which behaved as a tolerant genotype, a specifically nitrated polypeptide was found.
authors.affiliation: Dipartimento di Colture Arboree, Università degli Studi di Bologna, Viale Fanin 46, 40127 Bologna, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Cellini
authors.forename: Francisco Javier
authors.initials: FJ
authors.lastname: Corpas
authors.forename: Juan Bautista
authors.initials: JB
authors.lastname: Barroso
authors.forename: Andrea
authors.initials: A
authors.lastname: Masia
mesh_terms: D000222:Adaptation, Physiological / Q000502:physiology*; D000975:Antioxidants / Q000378:metabolism; D001639:Bicarbonates / Q000494:pharmacology*; D002678:Chimera; D002734:Chlorophyll / Q000378:metabolism; D005638:Fruit / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D005838:Genotype; D006861:Hydrogen Peroxide / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000378:metabolism*; D000090463:Iron Deficiencies; D009569:Nitric Oxide / Q000378:metabolism*; D018384:Oxidative Stress; D010641:Phenotype; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D027861:Prunus / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D026361:Reactive Nitrogen Species / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012987:Soil; D013312:Stress, Physiological
chemical_list: D000975:Antioxidants; D001639:Bicarbonates; D026361:Reactive Nitrogen Species; D017382:Reactive Oxygen Species; D012987:Soil; D002734:Chlorophyll; D009569:Nitric Oxide; D006861:Hydrogen Peroxide; D007501:Iron