title: Kazachstania ichnusensis sp. nov., a diploid homothallic ascomycetous yeast from Sardinian lentisk rhizosphere.
abstract: During an investigation of yeast biota in the rhizosphere of lentisk in Sardinian semi-arid areas, a strain was isolated that could not be assigned to any known species. The sequence of the D1/D2 domain of the large subunit rDNA gene revealed that the strain belonged to the genus Kazachstania and was phylogenetically related to a clade including Kazachstania aerobia, Kazachstania servazzii, Kazachstania solicola and Kazachstania unispora. The novel isolate differed from members of this clade in its ability to assimilate D-glucono-1,5-lactone and its very weak fermentation of glucose and sucrose; its assimilation profile was unique within the genus Kazachstania. Monosporal colonies were able to sporulate, indicating that the species is homothallic. It is proposed that the isolate represents a novel species, Kazachstania ichnusensis sp. nov., with LCF 1675(T) (=CBS 11859(T)) as type strain.
authors.affiliation: Department of Applied Biology - Microbiology Division, Borgo 20 Giugno, 74, I - 06121 Perugia, Italy.
authors.forename: Gianluigi
authors.initials: G
authors.lastname: Cardinali
authors.affiliation: Department of Applied Biology - Microbiology Division, Borgo 20 Giugno, 74, I - 06121 Perugia, Italy.
authors.forename: Livio
authors.initials: L
authors.lastname: Antonielli
authors.affiliation: Department of Applied Biology - Microbiology Division, Borgo 20 Giugno, 74, I - 06121 Perugia, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Corte
authors.affiliation: Department of Applied Biology - Microbiology Division, Borgo 20 Giugno, 74, I - 06121 Perugia, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Roscini
authors.affiliation: Department of Applied Biology - Microbiology Division, Borgo 20 Giugno, 74, I - 06121 Perugia, Italy.
authors.forename: Ambra
authors.initials: A
authors.lastname: Bagnetti
authors.affiliation: Department of Applied Biology - Microbiology Division, Borgo 20 Giugno, 74, I - 06121 Perugia, Italy.
authors.forename: Cristina
authors.initials: C
authors.lastname: Pelliccia
authors.affiliation: Department of Applied Biology - Microbiology Division, Borgo 20 Giugno, 74, I - 06121 Perugia, Italy.
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Puddu
mesh_terms: D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D005942:Gluconates / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D007783:Lactones / Q000378:metabolism; D008853:Microscopy; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D010802:Phylogeny; D058441:Rhizosphere; D004718:Saccharomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013395:Sucrose / Q000378:metabolism
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D005942:Gluconates; D007783:Lactones; D013395:Sucrose; D005947:Glucose; C010730:beta-glucono-1,5-lactone