title: [Spectrophotometric determination of piroxicam using ferric ferricyanide as reagent].
abstract: For the piroxicam determination (nonsteroidal antiinflammatory drug-NSAID) it was developed a spectrophotometric method, based on the reduction of ferric ferricyanide into ferro-ferricyanide (Prussian Blue), with maximum of absorbance at 760 nm.
  
  
  METHODS
  The practical working conditions were established. In the 0.2 divided by 2.0 microg/mL range of piroxicam concentration, were used the 2 mL of ferric ferricyanide 1 mL of 2N hydrocloric acid. To delay the flocculation of Prussian Blue it was to add a 1 mL solution of sodium lauryl sulfate 1%. After 15 minutes read the absorbance at 760 nm. The developed method was validated.
  
  
  RESULTS
  The method showed a good linearity in the range of 0.2 divided by 2.0 microg/mL (the correlation coefficient r = 0.9995). The detection limit (LD) was 0.056 microg/mL and the quantification limit (LQ) was 0.18 microg/mL. There were established the system precision (RSD = 0.25%), the precison (RSD = 1.91%) and the accuracy-recovery in the range 98.21 divided by 104.92% with a mean recovery of 100.91%.
  
  
  CONCLUSIONS
  The experimental results demonstrated a good sensibility. The specific absorptivity for this method is A(1 cm, 760 nm)(1%) = 4374 much higher than piroxicam in UV (A(1 cm, 330 nm)(1%) = 296)
authors.affiliation: Institutul Naţional de Cercetare-Dezvoltare pentru Microbiologie şi Imunologie, I. Cantacuzino" filiala Iaşi.
authors.forename: Mariana
authors.initials: M
authors.lastname: Mândrescu
authors.forename: A F
authors.initials: AF
authors.lastname: Spac
authors.forename: V
authors.initials: V
authors.lastname: Dorneanu
mesh_terms: D000465:Algorithms; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis; D004396:Coloring Agents / Q000737:chemistry*; D005292:Ferricyanides / Q000737:chemistry*; D005295:Ferrocyanides / Q000737:chemistry; D006851:Hydrochloric Acid / Q000737:chemistry; D007202:Indicators and Reagents / Q000737:chemistry*; D010894:Piroxicam / Q000032:analysis*; D015203:Reproducibility of Results; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D004396:Coloring Agents; D005292:Ferricyanides; D005295:Ferrocyanides; D007202:Indicators and Reagents; D010894:Piroxicam; D012967:Sodium Dodecyl Sulfate; D006851:Hydrochloric Acid; C000170:ferric ferrocyanide; C053439:ferric ferricyanide