title: [Laser tweezers Raman spectroscopy analysis of cold-adapted aromatic hydrocarbons-degradating strains isolated from Antarctic Sea].
abstract: Laser tweezers Raman spectroscopy can help with observing and studying individual cells or organelles in a natural state for a relatively long period. In the present experiment, Laser tweezers Raman spectroscopy (LTRS) was used as a tool to report physiological metabolism such as cells growth and nucleic acid, proteins, lipid and glucose of a single active cold-adapted Aromatic hydrocarbons-degradating strains isolated from Antarctic Sea. After the Raman spectrum was collected and analyzed, the findings are as follows: Raman spectrum identified the components of a single cold-adapted Aromatic hydrocarbons-degradating strain and there were more proteins and carbohydrate produced during the Planococcus sp. NJ41 and Shewanella sp. NJ49 growth and degradation; but there was more lipid than the proteins produced during the Pseudoalteromonas sp. NJ289 growth and degradation; the amount of proteins produced by the strains corresponds with the production of degradation rate-limiting enzyme, and was also related to the capacity of low-temperature degradation of aromatic hydrocarbons.
authors.affiliation: First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China. wangyb1983@163.com
authors.forename: Yi-Bin
authors.initials: YB
authors.lastname: Wang
authors.forename: Jin-Lai
authors.initials: JL
authors.lastname: Miao
authors.forename: Bi-Juan
authors.initials: BJ
authors.lastname: He
authors.forename: Qiang
authors.initials: Q
authors.lastname: Liang
authors.forename: Fang-Ming
authors.initials: FM
authors.lastname: Liu
authors.forename: Zhou
authors.initials: Z
authors.lastname: Zheng
mesh_terms: D000864:Antarctic Regions; D001419:Bacteria / Q000378:metabolism*; D006841:Hydrocarbons, Aromatic / Q000378:metabolism*; D052898:Optical Tweezers; D013059:Spectrum Analysis, Raman / Q000379:methods*; D014871:Water Microbiology
chemical_list: D006841:Hydrocarbons, Aromatic