title: Expression patterns of the steroid receptor coactivator family in human ovarian endometriosis.
abstract: OBJECTIVE
  Endometriosis is an estrogen-dependent disease that causes pelvic pain and infertility. In this study, to examine the estrogen-dependent mechanisms of human endometriosis, we focused on the expression patterns of the steroid receptor coactivator (SRC) family of cofactors for nuclear steroid receptors and estrogen receptor α (ERα).
  
  
  METHODS
  The expression patterns of SRC-1, transitional intermediary factor 2 (TIF2), SRC-3, and ERα, were analyzed by immunohistochemistry of normal endometrium and ovarian endometriotic tissue. In addition, reverse transcription polymerase chain reaction (RT-PCR) for the SRCs was performed for ovarian endometriosis.
  
  
  RESULTS
  SRCs were expressed in all examined tissues. The expression levels of SRC-1 and the number of SRC-1-positive cells in ovarian endometriosis were greater than those of TIF2 and SRC-3. In addition, immunohistochemistry showed that ERα was colocalized with SRC-1 in almost all glandular and many stromal cells in ovarian endometriotic tissue.
  
  
  CONCLUSIONS
  The present study demonstrates the expression pattern of SRCs in ovarian endometriosis. It appears that SRC-1 is predominant among the other SRC family members and colocalizes with ERα. Although further study is needed, SRC-1 may affect the transcriptional activity of ERα in human ovarian endometriosis.
authors.affiliation: Department of Obstetrics and Gynecology, School of Medicine, Fukushima Medical University, Fukushima, St. Luke's International Hospital, Tokyo, Japan. kumagami0017@yahoo.co.jp
authors.forename: Atsuko
authors.initials: A
authors.lastname: Kumagami
authors.forename: Akiko
authors.initials: A
authors.lastname: Ito
authors.forename: Hiromi
authors.initials: H
authors.lastname: Yoshida-Komiya
authors.forename: Keiya
authors.initials: K
authors.lastname: Fujimori
authors.forename: Akira
authors.initials: A
authors.lastname: Sato
mesh_terms: D000328:Adult; D004715:Endometriosis / Q000235:genetics / Q000378:metabolism*; D004717:Endometrium / Q000378:metabolism*; D047628:Estrogen Receptor alpha / Q000235:genetics / Q000378:metabolism; D005260:Female; D006801:Humans; D008875:Middle Aged; D056920:Nuclear Receptor Coactivator 1 / Q000235:genetics / Q000378:metabolism*; D051958:Nuclear Receptor Coactivator 2 / Q000235:genetics / Q000378:metabolism*; D056921:Nuclear Receptor Coactivator 3 / Q000235:genetics / Q000378:metabolism*; D010049:Ovarian Diseases / Q000235:genetics / Q000378:metabolism*; D010053:Ovary / Q000378:metabolism*
chemical_list: D047628:Estrogen Receptor alpha; C498001:NCOA2 protein, human; D051958:Nuclear Receptor Coactivator 2; C538866:NCOA3 protein, human; D056920:Nuclear Receptor Coactivator 1; D056921:Nuclear Receptor Coactivator 3