title: Amitriptyline induces coenzyme Q deficiency and oxidative damage in mouse lung and liver.
abstract: Amitriptyline is a tricyclic antidepressant commonly prescribed for the treatment of several neuropathic and inflammatory illnesses. We have already reported that amitriptyline has cytotoxic effect in human cell cultures, increasing oxidative stress, and decreasing growth rate and mitochondrial activity. Coenzyme Q (CoQ), a component of the respiratory chain and a potent antioxidant, has been proposed as a mitochondrial dysfunction marker. In the present work we evaluated lipid peroxidation, a consequence of oxidative stress, and CoQ level in liver, lung, kidney, brain, heart, skeletal muscle, and serum of mice treated with amitriptyline for two weeks. Lipid peroxidation was increased in a dose-dependent manner in all tissues analyzed. CoQ levels were increased in brain, heart, skeletal muscle, and serum, and strongly decreased in liver and lung. The relation between amitriptyline, CoQ, and oxidative stress is discussed.
authors.affiliation: Dpto. Citología e Histología Normal y Patológica, Facultad de Medicina, Universidad de Sevilla, Avda. Sánchez-Pizjuán s/n, 41009 Sevilla, Spain.
authors.forename: María Rosa
authors.initials: MR
authors.lastname: Bautista-Ferrufino
authors.forename: Mario D
authors.initials: MD
authors.lastname: Cordero
authors.forename: José Antonio
authors.initials: JA
authors.lastname: Sánchez-Alcázar
authors.forename: Matilde
authors.initials: M
authors.lastname: Illanes
authors.forename: Ana
authors.initials: A
authors.lastname: Fernández-Rodríguez
authors.forename: Plácido
authors.initials: P
authors.lastname: Navas
authors.forename: Manuel
authors.initials: M
authors.lastname: de Miguel
mesh_terms: D000639:Amitriptyline / Q000009:adverse effects* / Q000494:pharmacology; D000818:Animals; D000929:Antidepressive Agents, Tricyclic / Q000009:adverse effects* / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D008168:Lung / Q000187:drug effects* / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018384:Oxidative Stress / Q000187:drug effects*; D014451:Ubiquinone / Q000032:analysis / Q000172:deficiency* / Q000187:drug effects
chemical_list: D000929:Antidepressive Agents, Tricyclic; D014451:Ubiquinone; D000639:Amitriptyline