title: Increase in intracellular PGE2 induces apoptosis in Bax-expressing colon cancer cell.
abstract: BACKGROUND
  NSAIDs exhibit protective properties towards some cancers, especially colon cancer. Yet, it is not clear how they play their protective role. PGE2 is generally shown as the only target of the NSAIDs anticancerous activity. However, PGE2 known targets become more and more manifold, considering both the molecular pathways involved and the target cells in the tumour. The role of PGE2 in tumour progression thus appears complex and multipurpose.
  
  
  METHODS
  To gain understanding into the role of PGE2 in colon cancer, we focused on the activity of PGE2 in apoptosis in colon cancer cell lines.
  
  
  RESULTS
  We observed that an increase in intracellular PGE2 induced an apoptotic cell death, which was dependent on the expression of the proapoptotic protein Bax. This increase was induced by increasing PGE2 intracellular concentration, either by PGE2 microinjection or by the pharmacological inhibition of PGE2 exportation and enzymatic degradation.
  
  
  CONCLUSIONS
  We present here a new sight onto PGE2 in colon cancer cells opening the way to a new prospective therapeutic strategy in cancer, alternative to NSAIDs.
authors.affiliation: Département de Biologie Oncologique, Centre de Lutte Contre le Cancer René Gauducheau, Bd J, Monod, 44805 Nantes, Saint Herblain Cedex, France. lisenn.lalier@univ-nantes.fr
authors.forename: Lisenn
authors.initials: L
authors.lastname: Lalier
authors.forename: François
authors.initials: F
authors.lastname: Pedelaborde
authors.forename: Christophe
authors.initials: C
authors.lastname: Braud
authors.forename: Jean
authors.initials: J
authors.lastname: Menanteau
authors.forename: François M
authors.initials: FM
authors.lastname: Vallette
authors.forename: Christophe
authors.initials: C
authors.lastname: Olivier
mesh_terms: D017209:Apoptosis / Q000502:physiology*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000502:physiology; D003110:Colonic Neoplasms / Q000378:metabolism* / Q000473:pathology*; D051546:Cyclooxygenase 2 / Q000096:biosynthesis / Q000235:genetics; D052246:Cyclooxygenase 2 Inhibitors; D015232:Dinoprostone / Q000008:administration & dosage / Q000378:metabolism*; D045325:HCT116 Cells; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism; D019746:Intramolecular Oxidoreductases / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics; D008845:Microinjections; D000071876:Prostaglandin-E Synthases; D051028:bcl-2-Associated X Protein / Q000096:biosynthesis*
chemical_list: C494826:BAX protein, human; D052246:Cyclooxygenase 2 Inhibitors; D051028:bcl-2-Associated X Protein; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D019746:Intramolecular Oxidoreductases; D000071876:Prostaglandin-E Synthases; D015232:Dinoprostone