title: Inhibition of miR-214 expression represses proliferation and differentiation of C2C12 myoblasts.
abstract: MicroRNAs (miRNAs) are small non-coding RNAs that participate in diverse biological processes including skeletal muscle development. MiR-214 is an miRNA that is differentially expressed in porcine embryonic muscle and adult skeletal muscle, suggesting that miR-214 may be related to embryonic myogenesis. In this study, the myoblast cell line C2C12 was used for functional analysis of miR-214 in vitro. The results showed that miR-214 was expressed both in myoblasts and in myotubes and was upregulated during differentiation. After treatment with an miR-214 inhibitor and culturing in differentiation medium, myoblast differentiation was repressed, as indicated by the significant downregulation of expression of the myogenic markers myogenin and myosin heavy chain (MyHC). Interestingly, myoblast proliferation was also repressed when cells were transfected with an miR-214 inhibitor and cultured in growth medium by real-time proliferation assay and cell cycle analysis. Our results showed that miR-214 regulates both proliferation and differentiation of myoblasts depending on the conditions.
authors.affiliation: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education and Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, Huazhong Agricultural University, Wuhan, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Feng
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Cao
authors.forename: Xin-Yun
authors.initials: XY
authors.lastname: Li
authors.forename: Shu-Hong
authors.initials: SH
authors.lastname: Zhao
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics; D002460:Cell Line; D049109:Cell Proliferation; D003470:Culture Media; D051379:Mice; D008809:Mice, Inbred C3H; D035683:MicroRNAs / Q000502:physiology*; D024510:Muscle Development / Q000502:physiology; D018485:Muscle Fibers, Skeletal / Q000502:physiology; D032446:Myoblasts / Q000166:cytology* / Q000502:physiology; D018008:Myogenin / Q000378:metabolism; D018995:Myosin Heavy Chains / Q000378:metabolism; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D015854:Up-Regulation
chemical_list: D003470:Culture Media; D035683:MicroRNAs; D018008:Myogenin; D016376:Oligonucleotides, Antisense; D018995:Myosin Heavy Chains