title: Evaluation of Spoligotyping and MIRU-VNTR for Mycobacterium bovis in Xinjiang, China.
abstract: Mycobacterium bovis, the causative agent of bovine tuberculosis (bTB) in cattle, is also a pathogen for human and other mammals. In this study, 406 cows were screened for bTB by both single intradermal comparative cervical tuberculin (SICCT) test and IFN-γ assay. 135 M. bovis were isolated from 31 SICCT and IFN-γ double-positive cows in Xinjiang Uygur Autonomous Region of China. Spoligotyping and MIRU-VNTR were evaluated for genotyping, and 4 and 7 genotypes were identified, respectively. A new combination of nine MIRU-VNTR loci was most discriminative for M. bovis clones from Xinjiang. Interestingly, two new spoligotypes (SB1903 and SB1904) and special repeat numbers of three loci (ETR-D, QUB 1895 and QUB 3336) were discovered in this study. These results indicated a specific epidemic conservation in Xinjiang, China. M. bovis strains with the unique genotypes were isolated from the herds maintaining parent cows imported from the bTB-free countries, suggesting a possible transmission from the local breed of Xinjiang brown cattle.
authors.affiliation: Department of Bacteriology and Immunology, Beijing Tuberculosis & Thoracic Tumor Research Institute, Beijing 101149, China. sunzg75@163.com
authors.forename: Zhaogang
authors.initials: Z
authors.lastname: Sun
authors.forename: Rui
authors.initials: R
authors.lastname: Cao
authors.forename: Miao
authors.initials: M
authors.lastname: Tian
authors.forename: Xuxia
authors.initials: X
authors.lastname: Zhang
authors.forename: Xiyue
authors.initials: X
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Yuhui
authors.initials: Y
authors.lastname: Xu
authors.forename: Weixing
authors.initials: W
authors.lastname: Fan
authors.forename: Baoxu
authors.initials: B
authors.lastname: Huang
authors.forename: Chuanyou
authors.initials: C
authors.lastname: Li
mesh_terms: D000818:Animals; D002417:Cattle; D002681:China; D005838:Genotype; D018598:Minisatellite Repeats / Q000235:genetics*; D058889:Molecular Typing / Q000379:methods / Q000662:veterinary*; D009163:Mycobacterium bovis / Q000235:genetics*; D014380:Tuberculosis, Bovine / Q000382:microbiology*