title: Increased lipid production of the marine oleaginous microalgae Isochrysis zhangjiangensis (Chrysophyta) by nitrogen supplement.
abstract: Effects of nitrate feeding on the cell growth and lipid accumulation of marine microalgae Isochrysis zhangjiangensis were investigated. When nitrate was supplied at interval of 24h, instead of 72 h, a high lipid content of 40.9% and a biomass density of 3.1 g L(-1) were obtained. To confirm whether I. zhangjiangensis accumulates lipid during nitrogen-repletion, a two-stage cultivation method was applied. This algal strain had a high lipid content during sustained nitrate addition and showed a high carbohydrate content under nitrate-depletion conditions. These results revealed that this algal strain can accumulate lipids under nitrogen-repletion conditions and accumulate carbohydrate under nitrogen-depletion conditions. When cultured in an extremely high nitrate concentration, 9 g L(-1) at 24h intervals, the growth of algal cells was suppressed, but the highest lipid content of 53% was attained. This special characteristic of lipid accumulation makes I. zhangjiangensis an ideal candidate for producing biodiesel using N-rich wastewater.
authors.affiliation: Marine Bioproducts Engineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
authors.forename: Dina
authors.initials: D
authors.lastname: Feng
authors.forename: Zhaoan
authors.initials: Z
authors.lastname: Chen
authors.forename: Song
authors.initials: S
authors.lastname: Xue
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
mesh_terms: D056804:Biofuels; D018533:Biomass; D019149:Bioreactors; D004951:Esterification; D058087:Haptophyta / Q000254:growth & development / Q000378:metabolism*; D008055:Lipids / Q000096:biosynthesis*; D058086:Microalgae / Q000254:growth & development / Q000378:metabolism*; D009566:Nitrates / Q000378:metabolism*; D010788:Photosynthesis / Q000502:physiology
chemical_list: D056804:Biofuels; D008055:Lipids; D009566:Nitrates