title: The macrophage is a key factor in renal injuries caused by glomerular hyperfiltration.
abstract: Glomerular hyperfiltration is a common pathway leading to glomerulosclerosis in various kinds of kidney diseases. The 5/6 renal ablation is an established experimental animal model for glomerular hyperfiltration. On the other hand, low-grade inflammation is also a common mechanism for the progression of kidney diseases including diabetic nephropathy and atherosclerosis. Here we analyzed the gene expression profile in the remnant kidney tissues of 5/6 nephrectomized mice using a DNA microarray system and compared it with that of sham-operated control mice. The 5/6 nephrectomized mice showed glomerular hypertrophy and an increase in the extracellular matrix in the glomeruli. DNA microarray analysis indicated the up-regulated expression of various kinds of genes related to the inflammatory process in remnant kidneys. We confirmed the up-regulated expression of platelet factor-4, and monocyte chemoattractant protein-1, 2, and 5 in remnant kidneys by RT-PCR. The current results suggest that the inflammatory process is involved in the progression of glomerulosclerosis and is a common pathway of the pathogenesis of kidney disease.
authors.affiliation: Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8558, Japan.
authors.forename: Motofumi
authors.initials: M
authors.lastname: Sasaki
authors.forename: Kenichi
authors.initials: K
authors.lastname: Shikata
authors.forename: Shinichi
authors.initials: S
authors.lastname: Okada
authors.forename: Satoshi
authors.initials: S
authors.lastname: Miyamoto
authors.forename: Shingo
authors.initials: S
authors.lastname: Nishishita
authors.forename: Hitomi Usui
authors.initials: HU
authors.lastname: Kataoka
authors.forename: Chikage
authors.initials: C
authors.lastname: Sato
authors.forename: Jun
authors.initials: J
authors.lastname: Wada
authors.forename: Daisuke
authors.initials: D
authors.lastname: Ogawa
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Makino
mesh_terms: D000818:Animals; D018925:Chemokines / Q000235:genetics; D020869:Gene Expression Profiling; D005919:Glomerular Filtration Rate; D007668:Kidney / Q000473:pathology*; D008264:Macrophages / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D020411:Oligonucleotide Array Sequence Analysis; D012598:Sclerosis
chemical_list: D018925:Chemokines