title: Development of monoclonal antibodies that specifically interact with necrotic lymphoma cells.
abstract: The immune system has evolved mechanisms to sense not only microbes, but also necrotic cells. The pattern-recognition receptors in macrophages/dendritic cells that stimulate the acquired immune system are closely associated with danger signaling. In this study monoclonal antibodies (mAbs) that specifically interact with necrotic cells were developed. One IgG1 and two IgM mAbs were established, and they recognized a 80 kDa protein expressed in necrotic, but not live or apoptotic, cells. These mAbs, which serve as a probe for necrosis, facilitate analyses of the role of the immune complex that consists of necrotic cells and Ab and contributes to the formation of the inflammatory milieu induced by necrotic cell death.
authors.affiliation: Department of Microbiology and Immunology, Hokkaido University Graduate School of Medicine, Kita-ku, Sapporo, Japan.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Wakasa
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Shime
authors.forename: Mitsue
authors.initials: M
authors.lastname: Kurita-Taniguchi
authors.forename: Misako
authors.initials: M
authors.lastname: Matsumoto
authors.forename: Masahiro
authors.initials: M
authors.lastname: Imamura
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Seya
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000378:metabolism; D045744:Cell Line, Tumor; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology / Q000378:metabolism; D007075:Immunoglobulin M / Q000276:immunology / Q000378:metabolism; D008223:Lymphoma / Q000276:immunology* / Q000473:pathology; D008565:Membrane Proteins / Q000276:immunology* / Q000378:metabolism; D009336:Necrosis
chemical_list: D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008565:Membrane Proteins