title: miR-146a is modulated in human endothelial cell with aging.
abstract: BACKGROUND
  Increasing evidence has demonstrated that the senescence of vascular endothelial cells has critical roles in the pathogenesis of vascular dysfunction such as atherosclerosis and thrombosis. MicroRNA (miR) are small non-coding RNAs that inhibit gene expression by binding to complementary sequences in the 3'UTR of their target mRNAs. MiRs modulate a variety of biological functions such as cell development, cell differentiation, and apoptosis. Moreover, several miRs involved in endothelial cell function have been identified.
  
  
  RESULTS
  Through a microarray approach, we have identified a miR-146a that is progressively modulated in endothelial cells with aging. In young human umbilical vein endothelial cells, this miR is involved in a premature senescence-like phenotype through direct targeting of the NOX4 protein, implicated in cell senescence and aging.
  
  
  CONCLUSIONS
  Finding important factors that regulate endothelial cell senescence, like miR-146a, will help provide novel therapeutic strategies for vascular disorders.
authors.affiliation: Medical Research Council, Toxicology Unit, Leicester University, Leicester LE1 9HN, UK. mariuca.vasa-nicotera@ukb.uni-bonn.de
authors.forename: Mariuca
authors.initials: M
authors.lastname: Vasa-Nicotera
authors.forename: Hailan
authors.initials: H
authors.lastname: Chen
authors.forename: Paola
authors.initials: P
authors.lastname: Tucci
authors.forename: Ai Li
authors.initials: AL
authors.lastname: Yang
authors.forename: Gaelle
authors.initials: G
authors.lastname: Saintigny
authors.forename: Rossella
authors.initials: R
authors.lastname: Menghini
authors.forename: Christian
authors.initials: C
authors.lastname: Mahè
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Agostini
authors.forename: Richard A
authors.initials: RA
authors.lastname: Knight
authors.forename: Gerry
authors.initials: G
authors.lastname: Melino
authors.forename: Massimo
authors.initials: M
authors.lastname: Federici
mesh_terms: D002478:Cells, Cultured; D016922:Cellular Senescence / Q000235:genetics*; D020869:Gene Expression Profiling / Q000379:methods; D015971:Gene Expression Regulation, Enzymologic; D061307:Human Umbilical Vein Endothelial Cells / Q000378:metabolism*; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D000074663:NADPH Oxidase 4; D019255:NADPH Oxidases / Q000235:genetics / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D012333:RNA, Messenger / Q000378:metabolism; D014162:Transfection
chemical_list: C542542:MIRN146 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D000074663:NADPH Oxidase 4; D019255:NADPH Oxidases; C498910:NOX4 protein, human