title: Hepatoprotective properties of Caesalpinia sappan Linn. heartwood on carbon tetrachloride induced toxicity.
abstract: Aim of the study was to investigate the methanol and aqueous extracts of heartwood of C. sappan for its hepatoprotective activity against CCl4 induced toxicity in freshly isolated rat hepatocytes and animals. Freshly isolated rat hepatocytes were exposed to CCl4 (1%) along with/without various concentrations of methanolic and aqueous extract of C. sappan (1000-800 microg/ml) and the levels of selected liver enzymes were estimated. Antihepatotoxic effect of methanolic extract was observed in freshly isolated rat hepatocytes at concentrations 1000-800 microg/ml and was found to be similar to that of standard drug silymarin. Wistar strain albino rat model was used for the investigation of in vivo hepatoprotective properties of aqueous and methanolic extract of C. sappan (100 and 200 mg/kg body weight). Liver damage was induced by ip administration of CCl4 (30%) suspended in olive oil (1 ml/kg body weight). Both the tested extracts showed potent hepatoprotective activity at 200 mg/kg body weight test dose which was comparable with that of the standard silymarin used in similar test dose. The methanolic and aqueous extract was able to restore the biochemical levels to normal which were altered due to CCl4 intoxication in freshly isolated rat hepatocytes and also in animals.
authors.affiliation: Department of Pharmaceutical Biotechnology, J. S. S. College of Pharmacy, Ootacamund 643 001, India.
authors.forename: V Sathya
authors.initials: VS
authors.lastname: Srilakshmi
authors.forename: P
authors.initials: P
authors.lastname: Vijayan
authors.forename: P Vasanth
authors.initials: PV
authors.lastname: Raj
authors.forename: S A
authors.initials: SA
authors.lastname: Dhanaraj
authors.forename: H Raghu
authors.initials: HR
authors.lastname: Chandrashekhar
mesh_terms: D000818:Animals; D029911:Caesalpinia / Q000737:chemistry*; D002251:Carbon Tetrachloride; D002469:Cell Separation; D022781:Hepatocytes / Q000187:drug effects / Q000473:pathology; D008099:Liver / Q000187:drug effects* / Q000473:pathology*; D008107:Liver Diseases / Q000188:drug therapy / Q000473:pathology*; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D020011:Protective Agents / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D014934:Wood / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D020011:Protective Agents; D002251:Carbon Tetrachloride