title: Lhcb2 gene expression analysis in two ecotypes of Sedum alfredii subjected to Zn/Cd treatments with functional analysis of SaLhcb2 isolated from a Zn/Cd hyperaccumulator.
abstract: The Lhcb2 gene from hyperaccumulator Sedum alfredii was up-regulated more than three-fold while the non-hyperaccumulator accumulated one or two-fold higher amount of the mRNA than control plants under different concentrations of Cd(2+) for 24 h. Lhcb2 expression was up-regulated more than five-fold in a non-hyperaccumulator S. alfredii when exposed to 2 μM Cd(2+) or 50 μM Zn(2+) for 8 d and the hyperaccumulator had over two-fold more mRNA abundance than the control plants. Over-expression of SaLhcb2 increased the shoot biomass by 14-41% and the root biomass by 21-57% without Cd(2+) treatment. Four transgenic tobacco lines (L5, L7, L10 and L11) possessed higher shoot biomass than WT plants with Cd(2+). Four transgenic lines (L7, L8, L10 and L11) accumulated 6-35% higher Cd(2+) amounts in shoots than the wild type plants.
authors.affiliation: MOE Key Lab of Environmental Remediation and Ecosystem Health, College of Environmental & Resource Sciences, Zhejiang University, Hangzhou, Zhejiang 310029, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Takeshi
authors.initials: T
authors.lastname: Senoura
authors.forename: Xiaoe
authors.initials: X
authors.lastname: Yang
authors.forename: Yueen
authors.initials: Y
authors.lastname: Chao
authors.forename: Naoko K
authors.initials: NK
authors.lastname: Nishizawa
mesh_terms: D018533:Biomass; D002104:Cadmium / Q000378:metabolism*; D002413:Cations, Divalent / Q000378:metabolism; D061146:Chlorophyll Binding Proteins / Q000096:biosynthesis*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D018517:Plant Roots / Q000254:growth & development; D018520:Plant Shoots / Q000254:growth & development; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D031963:Sedum / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D017422:Sequence Analysis, DNA; D014026:Nicotiana / Q000201:enzymology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D015032:Zinc / Q000378:metabolism*
chemical_list: D002413:Cations, Divalent; D061146:Chlorophyll Binding Proteins; D012333:RNA, Messenger; D002104:Cadmium; D015032:Zinc