title: Correlation between insulin requirements and anti-galactose antibodies in patients with type 1 diabetes transplanted with neonatal pig islets.
abstract: Pig xenografts represent an alternative source of organs for transplantation. Immunosuppression can prevent rejection, but involves high risk and cost. New anti-rejection techniques have been developed; however, results have not been successful. Few studies have reported xenoantibody levels in xenotransplanted patients with diabetes and no patients have reported a clinical correlation. We analysed anti-galactose (Gal) and other anti-pig antibody (APA) levels in xenotransplanted patients with type 1 diabetes and the relation to the clinical outcome. Twenty-three patients with type 1 diabetes were transplanted with porcine islets inside a device without immunosuppression. Demographic characteristics, insulin dose and xenoantibody levels at different periods were recorded. Anti-Gal and anti-pig antibodies were measured through indirect enzyme-linked immunosorbent assay (ELISA) and haemolytic anti-pig antibody assay. More than 50% were female; the mean current age, current diabetes duration, diabetes duration at xenotransplantation and time post-transplantation were: 20·8, 11, 5·5 and 5·7 years, respectively. Insulin doses remained with a mean reduction greater than 33% in more than 50% of the patients. The lowest anti-Gal antibody levels were related to the highest insulin dose reductions. This relationship could be explained by the device, Sertoli cells and accommodation process.
authors.affiliation: Cell Therapy Laboratory, Medicine School, National Autonomous University of Mexico (UNAM).
authors.forename: R
authors.initials: R
authors.lastname: Esquivel-Pérez
authors.forename: A L
authors.initials: AL
authors.lastname: Rodriguez-Ventura
authors.forename: L M
authors.initials: LM
authors.lastname: Dorantes
authors.forename: B
authors.initials: B
authors.lastname: Ramírez-González
authors.forename: M G
authors.initials: MG
authors.lastname: López-Santos
authors.forename: R
authors.initials: R
authors.lastname: Valdes-Gonzalez
mesh_terms: D000293:Adolescent; D000818:Animals; D000831:Animals, Newborn; D000910:Antibodies, Heterophile / Q000097:blood; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000276:immunology* / Q000503:physiopathology / Q000628:therapy*; D004864:Equipment and Supplies; D005260:Female; D005500:Follow-Up Studies; D005690:Galactose / Q000276:immunology / Q000378:metabolism*; D006085:Graft Survival / Q000276:immunology; D006801:Humans; D007108:Immune Tolerance; D007328:Insulin / Q000378:metabolism*; D016381:Islets of Langerhans Transplantation / Q000295:instrumentation / Q000379:methods; D008297:Male; D020127:Recovery of Function; D012708:Sertoli Cells / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D013552:Swine; D014183:Transplantation, Heterologous; D055815:Young Adult
chemical_list: D000910:Antibodies, Heterophile; D007328:Insulin; D005690:Galactose