title: Heterologous expression, purification, and characterization of an α-mannosidase belonging to glycoside hydrolase family 99 of Shewanella amazonensis.
abstract: Shewanella amazonensis α-mannosidase (Sama99), a member of glycoside hydrolase family 99, was expressed in Escherichia coli. The purified Sama99 hydrolyzed pyridylamino (PA)-sugars, Glc₁Man₉GlcNAc₂-PA, and Glc₃Man₉GlcNAc₂-PA, and the product was probably a pyridylamino-decasaccharide in both cases. The mode of action of Sama99 was found to be essentially identical to that of rat endo-α-1,2-mannosidase, but the specificity of Sama99 was low.
authors.affiliation: Department of Applied Biological Science, Tokyo University of Agriculture and Technology, Japan.
authors.forename: Kana
authors.initials: K
authors.lastname: Matsuda
authors.forename: Yuma
authors.initials: Y
authors.lastname: Kurakata
authors.forename: Takatsugu
authors.initials: T
authors.lastname: Miyazaki
authors.forename: Ichiro
authors.initials: I
authors.lastname: Matsuo
authors.forename: Yukishige
authors.initials: Y
authors.lastname: Ito
authors.forename: Atsushi
authors.initials: A
authors.lastname: Nishikawa
authors.forename: Takashi
authors.initials: T
authors.lastname: Tonozuka
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015870:Gene Expression; D008969:Molecular Sequence Data; D051381:Rats; D020592:Shewanella / Q000201:enzymology* / Q000235:genetics; D013379:Substrate Specificity; D043323:alpha-Mannosidase / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*
chemical_list: D043323:alpha-Mannosidase