title: Effects of puffer (Sphoeroides rubripes) supplementation on disruption of antioxidant defense systems in ethanol-treated rats.
abstract: We investigated the effects of puffer (Sphoeroides rubripes) supplementation on antioxidant metabolism in ethanol-treated rats. Sprague-Dawley rats were randomly assigned into 4 groups of 7 rats each and fed (1) an AIN-93G diet (NC), (2) 25% ethanol (E), (3) 25% ethanol and an AIN-93G diet containing 1% puffer flesh (E+F), or (4) 25% ethanol and an AIN-93G diet containing 1% puffer skin (E+S) for 5 wk. At the end of the experimental period, the rats were sacrificed and their blood and organs were collected. To evaluate the effect of puffer supplementation, lipid-soluble antioxidant vitamin and conjugated diene (CD) levels, DNA damage, and mRNA expression of heme oxygenase-1 (HO-1) were assessed. Animals that were fed ethanol showed reduced plasma levels of lipid-soluble antioxidant vitamin and significantly increased levels of lipid peroxides, DNA damage, and HO-1 expression. Dietary supplementation with puffer conferred an antioxidant effect by significantly increasing the levels of γ-tocopherol, a lipid-soluble antioxidant vitamin, and by significantly decreasing the plasma levels of CD, DNA damage, and HO-1 expression. These results suggest that consumption of puffer improves the antioxidant status of ethanol-treated rats.
authors.affiliation: Department of Hotel Culinary and Bakery, Chang-shin College, Changwon, Gyeongnam, Korea.
authors.forename: Jong-Chan
authors.initials: JC
authors.lastname: Joo
authors.forename: Jae-Hee
authors.initials: JH
authors.lastname: Park
authors.forename: Rae-Young
authors.initials: RY
authors.lastname: Kim
authors.forename: Kyoung-Im
authors.initials: KI
authors.lastname: Jeon
authors.forename: Hyun-Jung
authors.initials: HJ
authors.lastname: Lee
authors.forename: Bo-Young
authors.initials: BY
authors.lastname: Seo
authors.forename: Eunju
authors.initials: E
authors.lastname: Park
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D004249:DNA Damage; D019587:Dietary Supplements; D000431:Ethanol / Q000008:administration & dosage*; D006419:Heme Oxygenase (Decyclizing) / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D015227:Lipid Peroxidation; D008099:Liver / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013154:Spleen / Q000378:metabolism; D023721:Tetraodontiformes; D024504:gamma-Tocopherol / Q000097:blood
chemical_list: D000975:Antioxidants; D000431:Ethanol; D024504:gamma-Tocopherol; D006419:Heme Oxygenase (Decyclizing); C491523:Hmox1 protein, rat