title: Intersectin1-s is involved in migration and invasion of human glioma cells.
abstract: Malignant gliomas have a tendency to invade diffusely into surrounding healthy brain tissues, thereby precluding their successful surgical removal. Intersectin1 (ITSN1) as a molecular linker in the central nervous system is well known as an important regulator of endocytosis and exocytosis. ITSN1 has two isoforms: ITSN1-l and ITSN1-s. In this study, we show that siRNA-mediated down regulation of ITSN1-s inhibited migration and invasion of glioma cells. In addition, we demonstrate the possible mechanisms by which ITSN1-s functions in migration and invasion. Several key proteins, including cofilin, LIMK, PAK, FAK, integrin β1, and MMP-9, which are critical for cells migration and invasion, were probably involved in ITSN1-s signaling pathways. These results suggest that ITSN1-s contributes to glioma cells migration and invasion by regulating the formation of cytoskeleton, influencing adhesion and increasing expression of MMP-9. Our results indicate that ITSN1-s is a critical factor in gliomas invasion and identify that ITSN1-s is a new potentially antiinvasion target for therapeutic intervention in gliomas.
authors.affiliation: Central Laboratory of Oncology Department, Tianjin Medical University Cancer Institute and Hospital, Tianjin, People's Republic of China.
authors.forename: Yongjie
authors.initials: Y
authors.lastname: Ma
authors.forename: Bingbing
authors.initials: B
authors.lastname: Wang
authors.forename: Wenliang
authors.initials: W
authors.lastname: Li
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Ting
authors.initials: T
authors.lastname: Ding
authors.forename: Jiao
authors.initials: J
authors.lastname: Zhang
authors.forename: Guoguang
authors.initials: G
authors.lastname: Ying
authors.forename: Li
authors.initials: L
authors.lastname: Fu
authors.forename: Feng
authors.initials: F
authors.lastname: Gu
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport / Q000235:genetics / Q000502:physiology*; D000818:Animals; D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D045744:Cell Line, Tumor; D002464:Cell Migration Inhibition / Q000235:genetics; D002465:Cell Movement / Q000235:genetics / Q000502:physiology; D004195:Disease Models, Animal; D005909:Glioblastoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology; D020033:Protein Isoforms / Q000235:genetics / Q000502:physiology; D034622:RNA Interference / Q000502:physiology
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; C485568:ITSN1 protein, human; D020033:Protein Isoforms