title: Experimental strategies for the analysis of D-amino acid containing peptides in crustaceans: a review.
abstract: Detection of D-amino acids in natural peptides has been, and remains a challenging task, as peptidyl isomerization is a peculiar and subtle posttranslational modification that does not induce any change in primary sequence or in physicochemical properties of the molecule such as molecular mass or pI. Therefore, the presence of a D-amino acid residue in a peptide chain is generally transparent to classical methods of peptide analysis (electrophoresis, chromatography, mass spectrometry, molecular biology). In this article, we will review the various experimental strategies and analytical techniques, which have been used to characterize and to study D-amino acid containing peptides in crustaceans.
authors.affiliation: Université Pierre et Marie Curie - Paris 06, Equipe Biogenèse des signaux peptidiques, ER3, 7 Quai Saint Bernard, F-75251, Paris cedex 05, France.
authors.forename: Daniel
authors.initials: D
authors.lastname: Soyez
authors.forename: Jean-Yves
authors.initials: JY
authors.lastname: Toullec
authors.forename: Nicolas
authors.initials: N
authors.lastname: Montagné
authors.forename: Céline
authors.initials: C
authors.lastname: Ollivaux
mesh_terms: D000596:Amino Acids / Q000032:analysis* / Q000737:chemistry; D000818:Animals; D002845:Chromatography; D003445:Crustacea / Q000737:chemistry*; D013058:Mass Spectrometry; D010455:Peptides / Q000737:chemistry*; D013237:Stereoisomerism
chemical_list: D000596:Amino Acids; D010455:Peptides