title: Enhanced transcription of estrogen receptor α and mitochondrial cytochrome b genes in uterine leiomyomas.
abstract: The relative expression levels of estrogen receptor α (ERα) and mitochondrial cytochrome b (MTCYB) transcripts and their association with ERα, -397T > C gene polymorphism was determined in premenopausal uterine leiomyomas and myometrium tissues to gain an insight into the role of ER-mediated action of estrogen on mitochondrial gene transcription. Both ERα and MTCYB transcripts were overexpressed in leiomyomas compared with myometrium tissues with 9.18 ± 0.79 folds and 5.24 ± 0.48 folds, respectively. ERα demonstrated ≥1.7 folds overexpression expressed over MTCYB (p < 0.001). Genotype correlation with transcript expression revealed that leiomyomas with CC genotype had significantly increased levels of ERα with 11.9 ± 1.02 folds as compared with 6.46 ± 0.56 folds seen in CT and TT genotypes together (p < 0.001). Interestingly, MTCYB transcript levels were also >1.9 folds overexpressed in leiomyomas with the CC genotype as compared with leiomyomas with other genotypes (p < 0.01).Significant elevation of ERα and MTCYB transcript levels in premenopausal leiomyomas and its association with ERα, -397 CC genotype suggests the mitochondrial-mediated role of estrogen as the promoter of leiomyoma tumorigenesis.
authors.affiliation: Department of Genetics, Vasavi Medical and Research Centre, Khairatabad, Hyderabad, 500 004, India.
authors.forename: Noor Ahmad
authors.initials: NA
authors.lastname: Shaik
authors.forename: Waseem Gul
authors.initials: WG
authors.lastname: Lone
authors.forename: Imran Ali
authors.initials: IA
authors.lastname: Khan
authors.forename: Kaipa Prabhakar
authors.initials: KP
authors.lastname: Rao
authors.forename: Vijaya Lakshmi
authors.initials: VL
authors.lastname: Kodati
authors.forename: Qurratulain
authors.initials: Q
authors.lastname: Hasan
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D045303:Cytochromes b / Q000235:genetics* / Q000378:metabolism; D047628:Estrogen Receptor alpha / Q000235:genetics* / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D050259:Genes, Mitochondrial / Q000235:genetics; D005838:Genotype; D006801:Humans; D007889:Leiomyoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism; D008954:Models, Biological; D009215:Myometrium / Q000378:metabolism / Q000473:pathology; D017697:Premenopause / Q000235:genetics; D014158:Transcription, Genetic / Q000502:physiology; D015854:Up-Regulation / Q000235:genetics; D014594:Uterine Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology
chemical_list: C506487:ESR1 protein, human; D047628:Estrogen Receptor alpha; D045303:Cytochromes b