title: [Study on fragmentation of vitexin and isorhamnetin-3-O-beta-D-rutinoside using electrospray quadrupole time of flight mass spectrometry].
abstract: OBJECTIVE
  To investigate the fragmentation pathway of vitexin and isorhamnetin-3-O-beta-D-rutinoside with CID-TOF-MS.
  
  
  METHODS
  Equipped with an LC-MS was carried out using an ultra-performance liquid chromatography, electrospray ionization quadrupole collision-induced dissociation-TOF-MS.
  
  
  RESULTS
  ESI-MS spectrum showed [M-H]- base peak of m/z 431. 0958 and m/z 623.1566. The CID-MS of vitexin showed five basic fragment ions, three of which corresponded to the glucosyl ring fracture: m/z 353, 341 and 311; other two were benzyl ion m/z 283, aglycone ion m/z 269. In addition, two low abundance ions, namely, m/z 161 and m/z 117, generated by RDA cracking ions, were also characteristic ions. The CID-MS of isorhamnetin-3-O-beta-D-rutinoside showed six main characteristic fragments ions corresponding to the loss of rhamnosyl m/z 477 and the glycosyl ring fracture: m/z 387, 357 and 311, and aglycone ion m/z 315. In addition, B ring generated m/z 300 and m/z 271 and C ring generated m/z 243 and the RDA cleavage generated m/z 151 and m/z 125.
  
  
  CONCLUSIONS
  Those fragment ions can be used to quickly identify vitexin and isorhamnetin-3-O-beta-D-rutinoside.
authors.affiliation: Guangdong Pharmnaceutical University, Guangzhou 510006, China. lkpchina@hotmail.com
authors.forename: Kunping
authors.initials: K
authors.lastname: Li
authors.forename: Chongkai
authors.initials: C
authors.lastname: Gao
authors.forename: Weiming
authors.initials: W
authors.lastname: Li
mesh_terms: D047310:Apigenin / Q000737:chemistry*; D004187:Disaccharides / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry*; D015394:Molecular Structure; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods*
chemical_list: D004187:Disaccharides; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; C515815:isorhamnetin 3-O-rutinoside; D047310:Apigenin; C032731:vitexin