title: Cloning, purification, crystallization and preliminary crystallographic analysis of the human histone deacetylase sirtuin 1.
abstract: Human sirtuin 1 is a member of the histone deacetylase family and is involved in cellular aging, tumourigenesis and cellular metabolism. Recombinant sirtuin 1 comprising residues 140-747 was crystallized using the hanging-drop vapour-diffusion method. The crystal diffracted to 3.4 Å resolution and belonged to space group P622, with unit-cell parameters a = b = 203.1, c = 625.3 Å, and is estimated to contain between six and 12 molecules per asymmetric unit.
authors.affiliation: Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, England.
authors.forename: Kenneth P
authors.initials: KP
authors.lastname: Holbourn
authors.forename: Matthew D
authors.initials: MD
authors.lastname: Lloyd
authors.forename: Andrew S
authors.initials: AS
authors.lastname: Thompson
authors.forename: Michael D
authors.initials: MD
authors.lastname: Threadgill
authors.forename: K Ravi
authors.initials: KR
authors.lastname: Acharya
mesh_terms: D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D006801:Humans; D056564:Sirtuin 1 / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification
chemical_list: C447939:SIRT1 protein, human; D056564:Sirtuin 1