title: Identification and characterization of CdgB, a diguanylate cyclase involved in developmental processes in Streptomyces coelicolor.
abstract: We describe the identification and functional characterization of cdgB (SCO4281), a recently discovered target of BldD, a key regulator of morphological differentiation and antibiotic production in the mycelial bacteria of the genus Streptomyces. cdgB (cyclic dimeric GMP [c-di-GMP] B) encodes a GGDEF-containing protein that has diguanylate cyclase activity in vitro. Consistent with this enzymatic activity, heterologous expression of cdgB in Escherichia coli resulted in increased production of extracellular matrix in colonies and enhanced surface attachment of cells in standing liquid cultures. In Streptomyces coelicolor, both overexpression and deletion of cdgB inhibited aerial-mycelium formation, and overexpression also inhibited production of the antibiotic actinorhodin, implicating c-di-GMP in the regulation of developmental processes in Streptomyces.
authors.affiliation: Department of Molecular Microbiology, John Innes Centre, Colney Lane, Norwich NR4 7UH, UK.
authors.forename: Ngat T
authors.initials: NT
authors.lastname: Tran
authors.forename: Chris D
authors.initials: CD
authors.lastname: Den Hengst
authors.forename: Juan Pablo
authors.initials: JP
authors.lastname: Gomez-Escribano
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Buttner
mesh_terms: D000880:Anthraquinones / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D019761:Phosphorus-Oxygen Lyases / Q000235:genetics / Q000378:metabolism*; D048372:Streptomyces coelicolor / Q000201:enzymology* / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D000095622:Benzoisochromanequinones
chemical_list: D000880:Anthraquinones; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D019761:Phosphorus-Oxygen Lyases; D014157:Transcription Factors; C111306:BldD protein, Streptomyces coelicolor; C060720:diguanylate cyclase; C013390:actinorhodin; D000095622:Benzoisochromanequinones