title: [Effect of miR-221-specific inhibitor on the proliferation and apoptosis of human colorectal carcinoma cells].
abstract: OBJECTIVE
  To investigate miRNA-221 expression in human colorectal carcinoma (CRC) cells and the effects of miR-221-specific inhibitor on the proliferation and apoptosis of CRC cells.
  
  
  METHODS
  Four human CRC cell lines (HT-29, Lovo, SW-480, and CaCO2) were examined for miRNA-221 expression using real-time Q-PCR. The specific 2,-methoxy-modified RNA oligonucleotides of miR-221 (anti-miR-221) were synthesized and transfected into Caco2 cells via liposome, and the changes in the expression of miR-221 in the cells were detected by real-time Q-PCR. The proliferation and apoptosis of the transfected CRC cells were detected using MTT assay and flow cytometry.
  
  
  RESULTS
  The 4 human CRC cells showed significantly upregulated expression of miR-221 compare with HUVECs (P<0.01). The miR-221-specific inhibitor, anti-miR-221, significantly inhibited the expression of miR-221 in Caco2 cells and suppressed the cell proliferation, causing also obvious cell apoptosis (P<0.01).
  
  
  CONCLUSIONS
  The miR-221-specific inhibitor shows potent inhibitory effect on the growth of CRC cells, suggesting its value as a potential anti-tumor candidate for treatment of CRC.
authors.affiliation: Department of General Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. wangwei0808@foxmail.com
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Kai
authors.initials: K
authors.lastname: Sun
authors.forename: Cheng-Tang
authors.initials: CT
authors.lastname: Wu
authors.forename: Shang-Tong
authors.initials: ST
authors.lastname: Lei
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Zeng
authors.forename: Ying-Jun
authors.initials: YJ
authors.lastname: Wu
authors.forename: Guo-Xin
authors.initials: GX
authors.lastname: Li
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D015179:Colorectal Neoplasms / Q000473:pathology*; D006801:Humans; D035683:MicroRNAs / Q000037:antagonists & inhibitors* / Q000378:metabolism*
chemical_list: D035683:MicroRNAs; C525709:MIR221, human