title: The association between GSTT1, M1, and P1 polymorphisms with coronary artery disease in Western Iran.
abstract: DNA damage which occurred by the effect of oxidant and mutant agents has an essential role in the development of atherosclerosis. To investigate the possible association between GSTs polymorphism with coronary artery disease (CAD), we investigated the frequency of GSTT1, M1, and P1 genotypes in patients with CAD compared to controls. The genotypes of GSTT1, M1, and P1 were determined in 209 angiographically documented CAD patients and 108 normal coronary artery cases (as controls) by Multiplex Polymerase Chain Reaction and PCR-RFLP. In CAD patients, the frequency of GSTT1-null genotype was significantly (P = 0.025) lower than that in control. The presence of this genotype was associated with 2.2-fold increased risk of CAD. However, the frequency of GSTM1 and GSTP1 genotypes were not significantly different comparing both groups (P = 0.405 and P = 0.521, respectively). Moreover, non smokers patients had a lower frequency of GSTM1-null genotype (29.2%) compared to non smoker controls (43.5%, P = 0.043). Also, the frequency of both GSTT1-null and GSTM1-null genotypes in patients (3.8%) was significantly lower compared to controls with the same genotypes (10.2%, P = 0.014). Our results indicated that a reduction in the frequency of GSTT1-null and GSTM1-null genotypes that observed in our study might be involved in the pathogenesis of CAD in our population.
authors.affiliation: Department of Biochemistry, Medical School, Kermanshah University of Medical Sciences, Kermanshah, Iran.
authors.forename: Hamid
authors.initials: H
authors.lastname: Nomani
authors.forename: Hadi
authors.initials: H
authors.lastname: Mozafari
authors.forename: Shahrokh Mohamadzadeh
authors.initials: SM
authors.lastname: Ghobadloo
authors.forename: Zohreh
authors.initials: Z
authors.lastname: Rahimi
authors.forename: Asad Vaisi
authors.initials: AV
authors.lastname: Raygani
authors.forename: Mehr Ali
authors.initials: MA
authors.lastname: Rahimi
authors.forename: Asghar Fadaei
authors.initials: AF
authors.lastname: Haghi
authors.forename: Ali Asghar
authors.initials: AA
authors.lastname: Keshavarz
mesh_terms: D000368:Aged; D001794:Blood Pressure; D016022:Case-Control Studies; D003324:Coronary Artery Disease / Q000097:blood / Q000235:genetics*; D005260:Female; D056726:Genetic Association Studies; D005838:Genotype; D051549:Glutathione S-Transferase pi / Q000235:genetics*; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D007492:Iran; D008055:Lipids / Q000097:blood; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D018709:Statistics, Nonparametric
chemical_list: D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase; D008055:Lipids; C413545:glutathione S-transferase T1; C496556:GSTP1 protein, human