title: Colocalization of gold nanoparticle-conjugated DNA hybridization for enhanced surface plasmon detection using nanograting antennas.
abstract: We have investigated enhanced surface plasmon resonance detection through colocalization of gold nanoparticle (GNP)-conjugated target molecules and near-fields established by nanograting-based antennas. The target colocalization was implemented by angled dielectric thin-film deposition on the nanograting structure. The concept was tested by detecting DNA hybridization and shows that the colocalization produces an additional 60%-80% increase of resonance shifts. The colocalization involves a much smaller number of target molecules, so that the measured enhancement per molecule by the colocalization of GNP-conjugated DNA oligomers was estimated to be by more than 2 orders of magnitude relative to that of thin-film-based conventional detection.
authors.affiliation: School of Electrical and Electronic Engineering, Yonsei University, Seoul, Korea 120-749.
authors.forename: Youngjin
authors.initials: Y
authors.lastname: Oh
authors.forename: Wonju
authors.initials: W
authors.lastname: Lee
authors.forename: Donghyun
authors.initials: D
authors.lastname: Kim
mesh_terms: D004247:DNA / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D009693:Nucleic Acid Hybridization; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D006046:Gold; D004247:DNA