title: Efficient production of a thermophilic 2-deoxyribose-5-phosphate aldolase in glucose-limited fed-batch cultivations of Escherichia coli by continuous lactose induction strategy.
abstract: The production of a thermophilic 2-deoxyribose-5-phosphate aldolases (DERA) in Escherichia coli BL21 under continuous lactose induction strategy was investigated. The process was combined with the exponential feeding method, controlling the feeding rate to maintain the specific growth rate at 0.15 h(-1). The results indicate that the lactose concentration in the feed medium affected directly the expression of the target protein. The use of 50 g/L in the feed medium resulted in the biomass concentration of 39.3 g DCW/L, and an expression level of above 30%, and the maximum final DERA concentration of 16,200 U/L. Furthermore, the acetate concentration remained at a low level in the fed-batch phase, less than 0.5 g/L. In conclusion, combining glucose feeding with lactose induction is a more powerful way to achieve high cell density cultures and to efficiently produce the thermophilic DERA. The results also indicate the potential industrial utility in the scale production of other recombinant proteins.
authors.affiliation: Center for Biomedicine and Health, Hangzhou Normal University, Hangzhou, 310012, People's Republic of China.
authors.forename: Xiao-Lin
authors.initials: XL
authors.lastname: Pei
authors.forename: Qiu-Yan
authors.initials: QY
authors.lastname: Wang
authors.forename: Cheng-Lu
authors.initials: CL
authors.lastname: Li
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Qiu
authors.forename: Kai-Lin
authors.initials: KL
authors.lastname: Xie
authors.forename: Li-Feng
authors.initials: LF
authors.lastname: Huang
authors.forename: An-Ming
authors.initials: AM
authors.lastname: Wang
authors.forename: Zhao-Wu
authors.initials: ZW
authors.lastname: Zeng
authors.forename: Tian
authors.initials: T
authors.lastname: Xie
mesh_terms: D000446:Aldehyde-Lyases / Q000096:biosynthesis* / Q000235:genetics; D018533:Biomass; D019149:Bioreactors; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D006358:Hot Temperature; D007218:Industrial Microbiology; D007785:Lactose / Q000378:metabolism / Q000494:pharmacology; D009876:Operon; D010957:Plasmids; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D015533:Transcriptional Activation / Q000187:drug effects*; D014169:Transformation, Bacterial
chemical_list: D011994:Recombinant Proteins; D000446:Aldehyde-Lyases; C020019:deoxyribose-phosphate aldolase; D005947:Glucose; D007785:Lactose