title: [Effect of local delivery of basic fibroblast growth factor 2 on osseointegration around implant in tibia of diabetic rats].
abstract: OBJECTIVE
  To evaluate the effect of the local delivery of basic fibroblast growth factor 2 (bFGF-2) on the osseointegration around titanium implant of diabetic rats.
  
  
  METHODS
  The bFGF-2-loaded poly (lactic-co-glycolic acid) microspheres were prepared by water/oil/water (W/O/W) double-emulsion solvent evaporation method. Thirty-five male SPF level Sprague Dawley rats, weighing 220-250 g and aged 9 weeks, were selected as experimental animals. Ten rats were fed with the routine diet as normal control group. The other 25 rats were made the diabetic animal model by giving high fat-sugar diet and a low dose streptozotocin (30 mg/kg) intravenously; 20 rats were made the diabetic animal model successfully. Then 20 rats were randomly divided into diabetic control group (n = 10) and bFGF-2 intervention group (n = 10). A hole was drilled in the right tibia bone of all rats, and the titanium implant treated by micro-arc oxidation surface was planted into the hole. Simultaneously, the previously prepared microspheres and blood were mixed and were loaded on the surface of the implant before it was implanted into the rats of the bFGF-2 intervention group. At 4 and 8 weeks, the tibia containing implants was harvested, embedded with resin and made undecalcified tissue slices to compare the osseointegration.
  
  
  RESULTS
  At 4 weeks, the implants of the normal control group were surrounded by new lamellar bone with continuity; whereas the tissue around the implants of the diabetic control group contained little woven bone and some fibrous tissue; and obvious new formed bone with continuity was observed in bFGF-2 intervention group. At 8 weeks, the results of 3 groups were similar to those at 4 weeks. At 4 weeks, the percentage of bone-implant contact (BIC) in diabetic control group was significantly less than those in normal control group (P < 0.05) and in bFGF-2 intervention group (P < 0.05); the BIC in bFGF-2 intervention group was less than in normal control group, but showing no significant difference (P > 0.05). After 8 weeks, the BIC in normal control group and in bFGF-2 intervention group were significantly greater than that in diabetic control group (P < 0.05), but there was no significant difference between bFGF-2 intervention group and normal control group (P > 0.05).
  
  
  CONCLUSIONS
  Local delivery of bFGF-2 around titanium implants may improve the osseointegration in diabetic rats.
authors.affiliation: Department of Oral Implantology, School of Stomatology, the Fourth Military Medical University, Xi'an Shaanxi, 710032, P.R.China.
authors.forename: Guike
authors.initials: G
authors.lastname: Zou
authors.forename: Yingliang
authors.initials: Y
authors.lastname: Song
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
authors.forename: Miao
authors.initials: M
authors.lastname: Yu
authors.forename: Lihua
authors.initials: L
authors.lastname: Liang
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage / Q000494:pharmacology*; D020007:Implants, Experimental; D008297:Male; D008863:Microspheres; D016348:Osseointegration / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013977:Tibia / Q000601:surgery*; D014025:Titanium
chemical_list: D016222:Fibroblast Growth Factor 2; D014025:Titanium