title: Accelerated turnover of taste bud cells in mice deficient for the cyclin-dependent kinase inhibitor p27Kip1.
abstract: BACKGROUND
  Mammalian taste buds contain several specialized cell types that coordinately respond to tastants and communicate with sensory nerves. While it has long been appreciated that these cells undergo continual turnover, little is known concerning how adequate numbers of cells are generated and maintained. The cyclin-dependent kinase inhibitor p27Kip1 has been shown to influence cell number in several developing tissues, by coordinating cell cycle exit during cell differentiation. Here, we investigated its involvement in the control of taste cell replacement by examining adult mice with targeted ablation of the p27Kip1 gene.
  
  
  RESULTS
  Histological and morphometric analyses of fungiform and circumvallate taste buds reveal no structural differences between wild-type and p27Kip1-null mice. However, when examined in functional assays, mutants show substantial proliferative changes. In BrdU incorporation experiments, more S-phase-labeled precursors appear within circumvallate taste buds at 1 day post-injection, the earliest time point examined. After 1 week, twice as many labeled intragemmal cells are present, but numbers return to wild-type levels by 2 weeks. Mutant taste buds also contain more TUNEL-labeled cells and 50% more apoptotic bodies than wild-type controls. In normal mice, p27 Kip1 is evident in a subset of receptor and presynaptic taste cells beginning about 3 days post-injection, correlating with the onset of taste cell maturation. Loss of gene function, however, does not alter the proportions of distinct immunohistochemically-identified cell types.
  
  
  CONCLUSIONS
  p27Kip1 participates in taste cell replacement by regulating the number of precursor cells available for entry into taste buds. This is consistent with a role for the protein in timing cell cycle withdrawal in progenitor cells. The equivalence of mutant and wild-type taste buds with regard to cell number, cell types and general structure contrasts with the hyperplasia and tissue disruption seen in certain developing p27Kip1-null sensory organs, and may reflect a compensatory capability inherent in the regenerative taste system.
authors.affiliation: Department of Anatomy and Cell Biology, Quillen College of Medicine, East Tennessee State University, Johnson City, TN, USA. harrisot@etsu.edu
authors.forename: Theresa A
authors.initials: TA
authors.lastname: Harrison
authors.forename: Lorraine B
authors.initials: LB
authors.lastname: Smith Adams
authors.forename: Preston D
authors.initials: PD
authors.lastname: Moore
authors.forename: Marla K
authors.initials: MK
authors.lastname: Perna
authors.forename: Jarrod D
authors.initials: JD
authors.lastname: Sword
authors.forename: Dennis M
authors.initials: DM
authors.lastname: Defoe
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D002452:Cell Count; D002453:Cell Cycle / Q000502:physiology*; D049109:Cell Proliferation; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D013650:Taste Buds / Q000166:cytology / Q000378:metabolism*
chemical_list: D050760:Cyclin-Dependent Kinase Inhibitor p27