title: Role of reactive oxygen species and proline cycle in anthraquinone accumulation in Rubia tinctorum cell suspension cultures subjected to methyl jasmonate elicitation.
abstract: Elicitors are compounds or factors capable of triggering a defense response in plants. This kind of response involves signal transduction pathways, second messengers and events such as Reactive Oxygen Species (ROS) generation, proline accumulation and secondary metabolite production. Anthraquinone (AQs) biosynthesis in Rubia tinctorum L. involves different metabolic routes, including shikimate and 2-C-methyl-d-erythritol-4-phosphate (MEP) pathways. It has been proposed that the proline cycle could be coupled with the pentose phosphate pathway (PPP), since the NADP+ generated by this cycle could act as a cofactor of the first enzymes of the PPP. The end-product of this pathway is erithrose-4-phosphate, which becomes the substrate of the shikimate pathway. The aim of this work was to study the effect of methyl jasmonate (MeJ), a well-known endogenous elicitor, on the PPP, the proline cycle and AQs production in R. tinctorum cell suspension cultures, and to elucidate the role of ROS in MeJ elicitation. Treatment with MeJ resulted in AQs as well as proline accumulation, which was mimicked by the treatment with a H₂O₂-generating system. Both MeJ-induced effects were abolished in the presence of diphenyliodonium (DPI), a NADPH oxidase inhibitor (main source of ROS). Treatment with the elicitor failed to induce PPP; therefore, this route did not turn out to be limiting the carbon flux to the shikimate pathway.
authors.affiliation: Cátedra de Microbiología Industrial y Biotecnología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, 1113 Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: María
authors.initials: M
authors.lastname: Perassolo
authors.forename: Carla Verónica
authors.initials: CV
authors.lastname: Quevedo
authors.forename: Víctor Daniel
authors.initials: VD
authors.lastname: Busto
authors.forename: Ana María
authors.initials: AM
authors.lastname: Giulietti
authors.forename: Julián Rodríguez
authors.initials: JR
authors.lastname: Talou
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D000880:Anthraquinones / Q000032:analysis / Q000378:metabolism*; D001713:Biphenyl Compounds / Q000494:pharmacology; D057486:Carbon Cycle; D002470:Cell Survival; D002478:Cells, Cultured; D003517:Cyclopentanes / Q000494:pharmacology*; D004896:Erythritol / Q000031:analogs & derivatives / Q000378:metabolism; D005969:Glutamate Dehydrogenase / Q000187:drug effects / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D007521:Isocitrate Dehydrogenase / Q000187:drug effects / Q000378:metabolism; D019255:NADPH Oxidases / Q000037:antagonists & inhibitors / Q000378:metabolism; D009861:Onium Compounds / Q000494:pharmacology; D054883:Oxylipins / Q000494:pharmacology*; D010427:Pentose Phosphate Pathway / Q000187:drug effects; D010937:Plant Growth Regulators / Q000494:pharmacology*; D057865:Plant Immunity; D011392:Proline / Q000032:analysis / Q000187:drug effects / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D045470:Rubia / Q000166:cytology / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D015398:Signal Transduction; D013403:Sugar Phosphates / Q000378:metabolism; D013997:Time Factors
chemical_list: C114232:2-C-methylerythritol 4-phosphate; D000085:Acetates; D000880:Anthraquinones; D001713:Biphenyl Compounds; D003517:Cyclopentanes; D009861:Onium Compounds; D054883:Oxylipins; D010937:Plant Growth Regulators; D017382:Reactive Oxygen Species; D013403:Sugar Phosphates; C031291:diphenyliodonium; C072239:methyl jasmonate; D011392:Proline; D006861:Hydrogen Peroxide; D007521:Isocitrate Dehydrogenase; D005969:Glutamate Dehydrogenase; D019255:NADPH Oxidases; D004896:Erythritol