title: The contribution of peripheral stem-loops to the catalytic activity of archaeal RNase P RNA from Pyrococcus horikoshii OT3.
abstract: We investigated the contribution of peripheral stem-loops to the catalytic activity of an archaeal RNase P RNA, PhopRNA, from Pyrococcus horikoshii OT3. PhopRNA mutants, in which the stem-loops were individually deleted, were prepared and characterized with respect to precursor tRNA (pre-tRNA) cleavage activity in the presence of five RNase P proteins. All the mutants retained the activity to some extent, indicating that they are moderately implicated in catalysis. Further characterization suggested that the stem-loops serve largely as binding sites for the proteins, and that their interactions are predominantly involved in stabilization of the active conformation of PhopRNA.
authors.affiliation: Laboratory of Biochemistry, Department of Bioscience and Biotechnology, Graduate School, Faculty of Agriculture, Kyushu University, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Hara
authors.forename: Atsushi
authors.initials: A
authors.lastname: Terada
authors.forename: Hiroki
authors.initials: H
authors.lastname: Yamaguchi
authors.forename: Takashi
authors.initials: T
authors.lastname: Nakashima
authors.forename: Yoshimitsu
authors.initials: Y
authors.lastname: Kakuta
authors.forename: Makoto
authors.initials: M
authors.lastname: Kimura
mesh_terms: D055162:Biocatalysis; D043902:Pyrococcus horikoshii / Q000201:enzymology*; D012322:RNA Precursors / Q000378:metabolism; D043262:Ribonuclease P / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D012322:RNA Precursors; D043262:Ribonuclease P