title: Vaccination of guinea pigs using mce operon mutants of Mycobacterium tuberculosis.
abstract: The limited efficacy of the BCG vaccine for tuberculosis, coupled with emerging information suggesting that it is poorly protective against newly emerging strains of Mycobacterium tuberculosis such as the W-Beijing isolates, makes it paramount to search for more potent alternatives. One such class of candidates is attenuated mutants derived from M. tuberculosis itself. We demonstrate here, in an initial short term assay, that mutants derived from disruption of the mce genes of the bacillus were highly protective in guinea pigs exposed by low dose aerosol infection with the virulent W-Beijing isolate SA161. This protection was demonstrated by a significant reduction in the numbers of bacilli harvested from the lungs, and dramatic improvements in lung histopathology.
authors.affiliation: Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA. aobregon@colostate.edu
authors.forename: Andrés
authors.initials: A
authors.lastname: Obregón-Henao
authors.forename: Crystal
authors.initials: C
authors.lastname: Shanley
authors.forename: María Verónica
authors.initials: MV
authors.lastname: Bianco
authors.forename: Angel A
authors.initials: AA
authors.lastname: Cataldi
authors.forename: Randall J
authors.initials: RJ
authors.lastname: Basaraba
authors.forename: Ian M
authors.initials: IM
authors.lastname: Orme
authors.forename: Fabiana
authors.initials: F
authors.lastname: Bigi
mesh_terms: D000818:Animals; D058491:Bacterial Load; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D005260:Female; D006168:Guinea Pigs; D006801:Humans; D008168:Lung / Q000382:microbiology / Q000473:pathology; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification / Q000472:pathogenicity; D009876:Operon / Q000235:genetics / Q000276:immunology; D013047:Specific Pathogen-Free Organisms; D016896:Treatment Outcome; D032581:Tuberculosis Vaccines / Q000008:administration & dosage* / Q000276:immunology; D014397:Tuberculosis, Pulmonary / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D014611:Vaccination; D014613:Vaccines, Attenuated / Q000008:administration & dosage* / Q000276:immunology
chemical_list: D001426:Bacterial Proteins; D032581:Tuberculosis Vaccines; D014613:Vaccines, Attenuated