title: Evaluation of a loop-mediated isothermal amplification assay for diagnosis of Clostridium difficile infections.
abstract: A new assay (illumigene C. difficile; Meridian Bioscience), based on the original loop-mediated isothermal amplification (LAMP) assay, was evaluated with 472 unformed stools from patients suspected of Clostridium difficile infection. Compared to the toxigenic culture, the sensitivity, specificity, and positive and negative predictive values were 91.8, 99.1, 91.8, and 99.1% for the illumigene C. difficile assay and 69.4, 100, 100, and 96.6% for the cytotoxicity assay, respectively.
authors.affiliation: National Reference Laboratory for Clostridium Difficile, Université Pierre et Marie Curie, Site Saint-Antoine, 27 rue de Chaligny, 75012 Paris, France. valerie.lalande@sat.aphp.fr
authors.forename: Valérie
authors.initials: V
authors.lastname: Lalande
authors.forename: Laurence
authors.initials: L
authors.lastname: Barrault
authors.forename: Sophie
authors.initials: S
authors.lastname: Wadel
authors.forename: Catherine
authors.initials: C
authors.lastname: Eckert
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Petit
authors.forename: Frédéric
authors.initials: F
authors.lastname: Barbut
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D016360:Clostridioides difficile / Q000235:genetics / Q000302:isolation & purification*; D003015:Clostridium Infections / Q000175:diagnosis* / Q000382:microbiology; D005243:Feces / Q000382:microbiology; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D013696:Temperature