title: Effects of prevention of oxidative and nitro-oxidative stress on experimental rat colon anastomosis using acetylcysteine, Ebselen and 1400w.
abstract: Oxygen radicals and radicals derived from nitrogen metabolism are important in wound and anostomotic healing. In particular, nitrous oxide, originating from induced nitrous oxide synthetase, retards the wound healing process by producing peroxynitride. Therefore induced nitric oxide synthase (INOS) inhibitors and peroxynitride cleansing agents seem helpful in promoting healing. The purpose of this study was to investigate the effects of N-acetylcysteine (antioxidant), ebselen (peroxynitride cleansing agent) and 1400w (INOS inhibitor) on experimental colonic anastomotic wound healing.
  
  
  METHODS
  45 randomized Sprague-Dawley rats received colonic anastomosis, and all animals were treated for four days with drugs specific for each group except for the sham and control groups. All rats were given a relaparatomy on the fifth day of the study and evaluated for study parameters indicating anastomotic healing, burst pressure, tissue malondialdehit (MDA), superoxide dismutase (SOD), glutathione peroxidase (GPx) and hydroxyproline (OH-proline).
  
  
  RESULTS
  when compared to the control group, increased (p < 0.01) burst pressure, OH-proline and decreased MDA, and SOD levels were noted in the 1400w group. Furthermore, the GPx levels were higher (p < 0.05) in rats given NAC therapy.
  
  
  CONCLUSIONS
  the positive results of selective INOS inhibition using 1400w in this study confirm the adverse effects of the INOS enzyme on anastomotic wound healing. Therefore, we have concluded that 1400w may be helpful in promoting anastomotic healing.
authors.affiliation: Gülhane Military Medical Academy, Department of General Surgery, Etlik, Ankara, Turkey.
authors.forename: Y
authors.initials: Y
authors.lastname: Poyrazoglu
authors.forename: T
authors.initials: T
authors.lastname: Yigit
authors.forename: A
authors.initials: A
authors.lastname: Harlak
authors.forename: O
authors.initials: O
authors.lastname: Mentes
authors.forename: S
authors.initials: S
authors.lastname: Gorgulu
authors.forename: A I
authors.initials: AI
authors.lastname: Uzar
authors.forename: O
authors.initials: O
authors.lastname: Kozak
mesh_terms: D000111:Acetylcysteine / Q000627:therapeutic use*; D000578:Amidines / Q000627:therapeutic use*; D000714:Anastomosis, Surgical; D000818:Animals; D001393:Azoles / Q000627:therapeutic use*; D001596:Benzylamines / Q000627:therapeutic use*; D003106:Colon / Q000601:surgery*; D016166:Free Radical Scavengers / Q000627:therapeutic use*; D054833:Isoindoles; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors*; D016566:Organoselenium Compounds / Q000627:therapeutic use*; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
chemical_list: D000578:Amidines; D001393:Azoles; D001596:Benzylamines; D016166:Free Radical Scavengers; D054833:Isoindoles; C104820:N-(3-(aminomethyl)benzyl)acetamidine; D016566:Organoselenium Compounds; C042986:ebselen; D019001:Nitric Oxide Synthase; D000111:Acetylcysteine