title: Microsatellite analysis of homozygosity progression of heterozygous genotypes segregating in the rice subspecies cross Pei'ai64s/Nipponbare.
abstract: Progression to homozygosity of heterozygous genotypes was studied in a cross of the rice subspecies Pei'ai64s and Nipponbare, using a set of 157 polymorphic microsatellite (SSR) markers. The segregation of heterozygous genotypes ranged from 49.13% in the F(2) population to 4.52% in the F(6) population (progression value 11.15%). The heterozygous genotypes were widely distributed in 180 F(2) plants, 330 F(6) lines, and 157 SSR markers. Homozygosity progression showed a wide distribution in plants and SSR markers but not in chromosomes. The segregation of heterozygous genotypes was not significant between populations but varied greatly in F(2) plants, F(6) lines, and SSR markers. The correlation between the progression to homozygosity and the heterozygosity of SSR markers was significant at the chromosome level. The segregation of heterozygous genotypes in plants, SSR markers, and chromosomes was not completely in accordance with Mendel's law. This information will help rice geneticists and breeders to understand heterozygous genotype segregation at the DNA level and to screen special markers for breeding.
authors.affiliation: Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 310006, China.
authors.forename: Yong-shu
authors.initials: YS
authors.lastname: Liang
authors.forename: Qi-jun
authors.initials: QJ
authors.lastname: Zhang
authors.forename: Shi-quan
authors.initials: SQ
authors.lastname: Wang
authors.forename: Li-yong
authors.initials: LY
authors.lastname: Cao
authors.forename: Zhi-qiang
authors.initials: ZQ
authors.lastname: Gao
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.forename: Shi-hua
authors.initials: SH
authors.lastname: Cheng
mesh_terms: D032461:Chromosomes, Plant / Q000235:genetics; D005819:Genetic Markers; D005838:Genotype; D006720:Homozygote; D006824:Hybridization, Genetic; D019656:Loss of Heterozygosity; D018895:Microsatellite Repeats; D012275:Oryza / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D005819:Genetic Markers