title: Suitable reference genes for quantitative real-time rt-pcr in total RNA extracted from canine whole blood using the PAXgene™ system.
abstract: Since blood cells produce various soluble factors like cytokines or chemokines, gene expression analysis in whole blood could be important to investigate disease pathogenesis. In gene expression analysis with quantitative real-time RT-PCR, accurate determination of relative mRNA transcription levels requires appropriate reference genes. To identify the optimal reference gene in canine whole blood, we compared transcription levels of twelve candidate reference genes in total RNA extracted using the PAXgene system. The stability of the reference gene was evaluated by three different statistical programs, GeNorm, Normfinder and Bestkeeper. The results indicated that SDHA, CG14980 and TBP were the most stably expressed genes, which can be used as optimal reference genes for gene expression analysis in canine whole blood.
authors.affiliation: The United Graduate School of Veterinary Sciences, Gifu University, 1–1 Yanagido, Gifu 501–1193, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Chimura
authors.forename: Sanae
authors.initials: S
authors.lastname: Shibata
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Kimura
authors.forename: Naho
authors.initials: N
authors.lastname: Kondo
authors.forename: Takashi
authors.initials: T
authors.lastname: Mori
authors.forename: Yuki
authors.initials: Y
authors.lastname: Hoshino
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kamishina
authors.forename: Sadatoshi
authors.initials: S
authors.lastname: Maeda
mesh_terms: D000818:Animals; D004285:Dogs / Q000097:blood / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods / Q000662:veterinary*; D008297:Male; D012313:RNA / Q000097:blood*; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary*; D012015:Reference Standards
chemical_list: D012313:RNA