title: Quantification of specific E. coli in gut mucosa from Crohn's disease patients.
abstract: We here present a method based on qRT-PCR to quantify E. coli LF82 in intestinal human samples. Two different primer-probe sets were designed to detect LF82, and a third to target total E. coli. The assay showed high robustness and specificity for detection of LF82 in the presence of intestinal tissue.
authors.affiliation: Center for Biological Sequence Analysis, Department of Systems Biology, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark. SJEN0236@heh.regionh.dk
authors.forename: Stina Rikke
authors.initials: SR
authors.lastname: Jensen
authors.forename: Lisbeth Nielsen
authors.initials: LN
authors.lastname: Fink
authors.forename: Carsten
authors.initials: C
authors.lastname: Struve
authors.forename: Claus
authors.initials: C
authors.lastname: Sternberg
authors.forename: Jens Bo
authors.initials: JB
authors.lastname: Andersen
authors.forename: Jørn
authors.initials: J
authors.lastname: Brynskov
authors.forename: Ole Haagen
authors.initials: OH
authors.lastname: Nielsen
authors.forename: Susanne
authors.initials: S
authors.lastname: Brix
mesh_terms: D003424:Crohn Disease / Q000382:microbiology*; D017931:DNA Primers / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007413:Intestinal Mucosa / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D017931:DNA Primers