title: [Suppressive effect of propofol on low-voltage-activated calcium currents in rat hippocampal neurons].
abstract: OBJECTIVE
  To investigate the effect of propofol on the low-voltage-activated calcium currents [ICa(LVA)] in rat hippocampal neurons.
  
  
  METHODS
  Hippocampal neurons were prepared from Wistar rats and cultured. ICa(LVA) was recorded using whole cell patch clamp technique. Different concentrations of propofol were added to the culture. The effect of propofol on ICa(LVA) was evaluated.
  
  
  RESULTS
  ICa(LVA) was inhibited by propofol in a concentration-dependent manner. Propofol 3 μmol /L had no effect on ICa(LVA). Propofol 10, 30, 100 and 300 μmol/L reduced peak ICa(LVA) by (12.6 ± 4.1)%, (29.2 ± 5.7)%, (36.6 ± 5.3)%, (31.6 ± 2.6)% respectively with a mean IC50 of 16.8 μmol/L and Hill coefficient of 0.15. The V1/2 of activation curve was shifted from (-10 ± 1 )mV to (-11 ± 2 )mV. K was 12 ± 1 and 8 ± 1. The V1/2 of inactivation curve was shifted from (-25 ± 1) mV to (-25 ± 5) mV. K was 15 ± 1 and 16 ± 3.
  
  
  CONCLUSIONS
  Propofol produces significant inhibition of ICa (LVA) calcium currents in the central neurons which may partly explain the anesthefic action of propofol on the central nervous system.
authors.affiliation: Department of Anesthesiology, Peking University School of Oncology, Beijing 100142, China. maggitan@163.com
authors.forename: Hong-yu
authors.initials: HY
authors.lastname: Tan
authors.forename: Tie-hu
authors.initials: TH
authors.lastname: Ye
authors.forename: Shi-ping
authors.initials: SP
authors.lastname: Ma
authors.forename: Xiao-liang
authors.initials: XL
authors.lastname: Wang
mesh_terms: D018686:Anesthetics, Intravenous / Q000494:pharmacology*; D000818:Animals; D015220:Calcium Channels / Q000187:drug effects* / Q000502:physiology; D002478:Cells, Cultured; D003864:Depression, Chemical; D004305:Dose-Response Relationship, Drug; D006624:Hippocampus / Q000166:cytology* / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D015742:Propofol / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D018686:Anesthetics, Intravenous; D015220:Calcium Channels; D015742:Propofol