title: Dynamic lineage analysis of embryonic morphogenesis using transgenic quail and 4D multispectral imaging.
abstract: We describe the development of transgenic quail that express various fluorescent proteins in targeted manners and their use as a model system that integrates advanced imaging approaches with conventional and emerging molecular genetics technologies. We also review the progression and complications of past fate mapping techniques that led us to generate transgenic quail, which permit dynamic imaging of amniote embryogenesis with unprecedented subcellular resolution.
authors.affiliation: Department of Biology and the Biological Imaging Center, California Institute of Technology, Pasadena, California 91125, USA.
authors.forename: Danielle V
authors.initials: DV
authors.lastname: Bower
authors.forename: Yuki
authors.initials: Y
authors.lastname: Sato
authors.forename: Rusty
authors.initials: R
authors.lastname: Lansford
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D019070:Cell Lineage / Q000235:genetics*; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000378:metabolism; D047108:Embryonic Development / Q000235:genetics*; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D057054:Molecular Imaging; D009024:Morphogenesis / Q000235:genetics*; D021381:Protein Transport / Q000235:genetics; D011784:Quail / Q000196:embryology / Q000235:genetics; D059008:Time-Lapse Imaging
chemical_list: D008164:Luminescent Proteins