title: Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers.
abstract: We have developed simultaneous detection of eight genes associated with the five categories of diarrheagenic Escherichia coli by the multiplex PCR assay with Alexa Fluor-labeled primers. This assay can easily distinguish eight genes based on the size and color of amplified products without gel staining.
authors.affiliation: Center for Public Health and Environment, Hiroshima Prefectural Technology Research Institute, 1-6-29, Minami-machi, Minami-ku, Hiroshima 734-0007, Japan. m-kuwayama89298@pref.hiroshima.lg.jp
authors.forename: Masaru
authors.initials: M
authors.lastname: Kuwayama
authors.forename: Naoki
authors.initials: N
authors.lastname: Shigemoto
authors.forename: Sachiko
authors.initials: S
authors.lastname: Oohara
authors.forename: Yukie
authors.initials: Y
authors.lastname: Tanizawa
authors.forename: Hiroko
authors.initials: H
authors.lastname: Yamada
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Takeda
authors.forename: Takeshi
authors.initials: T
authors.lastname: Matsuo
authors.forename: Shinji
authors.initials: S
authors.lastname: Fukuda
mesh_terms: D017931:DNA Primers / Q000737:chemistry / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics* / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000382:microbiology*; D029968:Escherichia coli Proteins / Q000235:genetics*; D005456:Fluorescent Dyes / Q000737:chemistry; D016133:Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods*; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D017931:DNA Primers; D029968:Escherichia coli Proteins; D005456:Fluorescent Dyes; D037521:Virulence Factors