title: Production of (R)-3-hydroxybutyric acid by fermentation and bioconversion processes with Azohydromonas lata.
abstract: Feasibility of producing (R)-3-hydroxybutyric acid ((R)-3-HB) using wild type Azohydromonas lata and its mutants (derived by UV mutation) was investigated. A. lata mutant (M5) produced 780 m g/l in the culture broth when sucrose was used as the carbon source. M5 was further studied in terms of its specificity with various bioconversion substrates for production of (R)-3-HB. (R)-3-HB concentration produced in the culture broth by M5 mutant was 2.7-fold higher than that of the wild type strain when sucrose (3% w/v) and (R,S)-1,3-butanediol (3% v/v) were used as carbon source and bioconversion substrate, respectively. Bioconversion of resting cells (M5) with glucose (1% v/w), ethylacetoacetate (2% v/v), and (R,S)-1,3-butanediol (3% v/v), resulted in (R)-3-HB concentrations of 6.5 g/l, 7.3g/l and 8.7 g/l, respectively.
authors.affiliation: Okinawa Industrial Technology Center, Suzaki 12-2, Uruma, Okinawa 904-2234, Japan. ugutyaru@pref.okinawa.lg.jp
authors.forename: Charles U
authors.initials: CU
authors.lastname: Ugwu
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Tokiwa
authors.forename: Toshio
authors.initials: T
authors.lastname: Ichiba
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000096:biosynthesis*; D042401:Alcaligenaceae / Q000235:genetics / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods*; D002072:Butylene Glycols; D003470:Culture Media; D005285:Fermentation; D016296:Mutagenesis / Q000528:radiation effects; D009154:Mutation / Q000235:genetics; D013045:Species Specificity; D013395:Sucrose; D014466:Ultraviolet Rays
chemical_list: D002072:Butylene Glycols; D003470:Culture Media; C028491:1,3-butylene glycol; D013395:Sucrose; D020155:3-Hydroxybutyric Acid