title: [Molecular and cellular consequences of the Chernobyl accident].
abstract: In this paper the results of the Chernobyl accident investigation 5-10 and 24 years after are summarized. The genomic instability, adaptive response formation, genome damage and oxidative status have been investigated. The studies were performed on cells in culture, mice, children and adults living in contaminated areas and liquidators. On cells in culture after exposition in the accident zone and culturing thereafter in laboratory conditions the cell proliferative activity decrease; the late cell death, the frequency of cells with micronuclei and giant cells increasing have been observed. In the progeny of exposed cells the enhancement of radiosensitivity has been noticed. So we can suppose that in cultured cells exposition in the zone of the accident the genomic instability is induced which results in many disturbances. At the organism level in mice exposed in the Chernobyl zone the radiosensitivity increase and the decrease of endotheliocytes density in brain tissue has been observed. On the stimulated by PHA blood lymphocytes of children the increase of the frequency of cells with micronuclei more than 2 time have been noticed. In all groups investigated, the decrease of individuals with significant adaptive response was observed. In children and adults inhabitants the increase of radiosensitivity after low dose of irradiation has been noticed. 24-year after the accident it was discovered that in liquidators lymphocytes the frequency of cells with micronuclei, with chromosome type aberrations, with DNA double strand breaks have been increased; the reactive oxygen species (ROS) were decreased in comparison with the control population. We can suppose that genomic instability induced in residents of contaminated regions and liquidators long after the accident results in the genetic apparatus damage, radiosensitivity enhancement, hypoxia that represent risk factors and increase the probability of tumour and non-tumour diseases. The development of these pathological processes may happen in much more remote periods.
authors.forename: I I
authors.initials: II
authors.lastname: Pelevina
authors.forename: G G
authors.initials: GG
authors.lastname: Afanas'ev
authors.forename: A V
authors.initials: AV
authors.lastname: Aleshchenko
authors.forename: M M
authors.initials: MM
authors.lastname: Antoshchina
authors.forename: V Ia
authors.initials: VIa
authors.lastname: Gotlib
authors.forename: A A
authors.initials: AA
authors.lastname: Konradov
authors.forename: O V
authors.initials: OV
authors.lastname: Kudriashova
authors.forename: E Iu
authors.initials: EIu
authors.lastname: Lizunova
authors.forename: A N
authors.initials: AN
authors.lastname: Osipov
authors.forename: N I
authors.initials: NI
authors.lastname: Riabchenko
authors.forename: A M
authors.initials: AM
authors.lastname: Serebrianyĭ
mesh_terms: D000328:Adult; D000818:Animals; D001921:Brain / Q000528:radiation effects / Q000648:ultrastructure; D002470:Cell Survival / Q000528:radiation effects; D002478:Cells, Cultured; D047852:Chernobyl Nuclear Accident; D002648:Child; D005720:Gamma Rays / Q000009:adverse effects*; D042822:Genomic Instability / Q000528:radiation effects*; D006801:Humans; D008214:Lymphocytes / Q000528:radiation effects* / Q000648:ultrastructure; D051379:Mice; D048629:Micronuclei, Chromosome-Defective / Q000528:radiation effects*; D011833:Radiation Injuries, Experimental / Q000209:etiology; D011836:Radiation Tolerance / Q000528:radiation effects; D016019:Survival Analysis; D013997:Time Factors; D014455:Ukraine