title: Cellular Injury of Cardiomyocytes during Hepatocyte Growth Factor Gene Transfection with Ultrasound-Triggered Bubble Liposome Destruction.
abstract: We transfected naked HGF plasmid DNA into cultured cardiomyocytes using a sonoporation method consisting of ultrasound-triggered bubble liposome destruction. We examined the effects on transfection efficiency of three concentrations of bubble liposome (1 × 10(6), 1 × 10(7), 1 × 10(8)/mL), three concentrations of HGF DNA (60, 120, 180 μg/mL), two insonification times (30, 60 sec), and three incubation times (15, 60, 120 min). We found that low concentrations of bubble liposome and low concentrations of DNA provided the largest amount of the HGF protein expression by the sonoporated cardiomyocytes. Variation of insonification and incubation times did not affect the amount of product. Following insonification, cardiomyocytes showed cellular injury, as determined by a dye exclusion test. The extent of injury was most severe with the highest concentration of bubble liposome. In conclusion, there are some trade-offs between gene transfection efficiency and cellular injury using ultrasound-triggered bubble liposome destruction as a method for gene transfection.
authors.affiliation: Department of Cardiovascular Dynamics, Research Institute, National Cerebral and Cardiovascular Center, 5-7-1 Fujishiro-dai,Suita, Osaka 565-8565, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Komamura
authors.forename: Rie
authors.initials: R
authors.lastname: Tatsumi
authors.forename: Yuko
authors.initials: Y
authors.lastname: Tsujita-Kuroda
authors.forename: Takatoshi
authors.initials: T
authors.lastname: Onoe
authors.forename: Kunio
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Nakamura
authors.forename: Jun-Ichi
authors.initials: J
authors.lastname: Miyazaki
authors.forename: Takeshi
authors.initials: T
authors.lastname: Horio
authors.forename: Masaru
authors.initials: M
authors.lastname: Sugimachi