title: [Effects of TNF-α on ICAM-1 and LFA-1 expression in peripheral blood mononuclear cells of children with febrile seizures].
abstract: OBJECTIVE
  To study the effects of TNF-α on ICAM-1 and LFA-1 expression in peripheral blood mononuclear cells (PBMC) of children with febrile seizures (FS).
  
  
  METHODS
  Sixteen children with FS and 16 age- and gender-matched healthy children were enrolled. The samples of PBMC from FS children were randomized into two groups with or without TNF-α treatment (TNF-α concentration 1.0 ng/mL). PBMC were purified and cultured with a conventional method in vitro. The expression of ICAM-1 and LFA-1 in PBMC was determined by flow cytometry (FCM).
  
  
  RESULTS
  ICAM-1［(20±9)% vs (14±7)%)］and LFA-1［(43±16)% vs (30±16)%］expression in PBMC in the untreated FS group was significantly higher than that in the normal control group (P<0.05). Compared with the untreated FS group, the treatment with TNF-α remarkably increased the ICAM-1 expression［(27±11)%］(P<0.05). PBMC LFA-1 expression［(52±21)%］in the TNF-α-treated group was higher than that in the untreated FS group, although there were no statistical differences between the two groups.
  
  
  CONCLUSIONS
  TNF-α treatment may increase LFA-1 and ICAM-1 expression in PBMC of children with FS.
authors.affiliation: Department of Neurology, Wuhan Children's Hospital, Wuhan 430016, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Sun
authors.forename: Zhi-Sheng
authors.initials: ZS
authors.lastname: Liu
authors.forename: Hui
authors.initials: H
authors.lastname: Yao
authors.forename: Shi-Xiu
authors.initials: SX
authors.lastname: Kang
authors.forename: Cai-Ying
authors.initials: CY
authors.lastname: He
authors.forename: Jia-Sheng
authors.initials: JS
authors.lastname: Hu
authors.forename: Ge-Fei
authors.initials: GF
authors.lastname: Wu
authors.forename: Fang-Lin
authors.initials: FL
authors.lastname: Wang
mesh_terms: D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D018799:Intercellular Adhesion Molecule-1 / Q000097:blood*; D007963:Leukocytes, Mononuclear / Q000737:chemistry* / Q000187:drug effects; D016169:Lymphocyte Function-Associated Antigen-1 / Q000097:blood*; D008297:Male; D003294:Seizures, Febrile / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
chemical_list: D016169:Lymphocyte Function-Associated Antigen-1; D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1