title: Effects of lipopolysaccharide and CpG-DNA on burn-induced skin injury.
abstract: Destruction of the skin barrier by thermal injury induces microbial invasion, which can lead to the development of systemic infection and septic shock. Microbial pathogens possess pathogen-associated molecular patterns (PAMPs), which are recognized by conserved receptors. To understand the role of PAMPs in thermal injury-induced mice, LPS or CpG-DNA were topically applied to dorsal skin after thermal injury. We observed an increase in the number of inflammatory cell infiltrates as well as thickening in the dermis upon treatment with LPS or CpG-DNA. We also found that expression of IL-1β, MIP-2, and RANTES induced by thermal injury was enhanced by LPS or CpG-DNA. In addition, the proportions of CD4(+) and CD8(+) T cells in the spleen and lymph nodes were altered by LPS or CpG-DNA. These results provide important information concerning PAMPs-induced inflammation upon thermal injury and provide a basis for studying the role of PAMPs in thermal injury-induced complications.
authors.affiliation: Department of Microbiology, College of Medicine, Hallym University, Chuncheon 200-702, Korea.
authors.forename: Byoung Kwon
authors.initials: BK
authors.lastname: Park
authors.forename: Dongbum
authors.initials: D
authors.lastname: Kim
authors.forename: Sunhee
authors.initials: S
authors.lastname: Cho
authors.forename: Jae-Nam
authors.initials: JN
authors.lastname: Seo
authors.forename: Jae-Bong
authors.initials: JB
authors.lastname: Park
authors.forename: Yong-Sun
authors.initials: YS
authors.lastname: Kim
authors.forename: Ihn-Geun
authors.initials: IG
authors.lastname: Choi
authors.forename: Hyeok-Yil
authors.initials: HY
authors.lastname: Kwon
authors.forename: Younghee
authors.initials: Y
authors.lastname: Lee
authors.forename: Hyung-Joo
authors.initials: HJ
authors.lastname: Kwon
mesh_terms: D000818:Animals; D002056:Burns / Q000150:complications*; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D018946:Chemokine CCL5 / Q000378:metabolism; D054426:Chemokine CXCL2 / Q000378:metabolism; D004247:DNA / Q000494:pharmacology; D004195:Disease Models, Animal; D053583:Interleukin-1beta / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology*; D012867:Skin / Q000276:immunology* / Q000293:injuries* / Q000473:pathology
chemical_list: C408982:CPG-oligonucleotide; D018946:Chemokine CCL5; D054426:Chemokine CXCL2; C404915:Cxcl2 protein, mouse; D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D009838:Oligodeoxyribonucleotides; D004247:DNA