title: Aquipuribacter hungaricus gen. nov., sp. nov., an actinobacterium isolated from the ultrapure water system of a power plant.
abstract: A Gram-positive actinobacterium, strain IV-75(T), was isolated by using R2A agar from the ultrapure water system of a power plant in Hungary. The strain exhibited a rod-coccus cell cycle, and was strictly aerobic, non-motile, catalase-positive and oxidase-negative. 16S rRNA gene sequence analysis revealed that strain IV-75(T) belonged to the suborder Micrococcineae and clustered with members of the family Intrasporangiaceae. Its closest phylogenetic neighbour was Arsenicicoccus bolidensis CCUG 47306(T) (94.3% 16S rRNA gene sequence similarity). The peptidoglycan of strain IV-75(T) contained meso-diaminopimelic acid and MK-10(H(4)) was the major menaquinone. The polar lipid pattern contained phosphatidylglycerol, two unidentified phospholipids, one glycolipid and several other lipid components. The major fatty acids were anteiso-C(15:0), C(18:1)ω9c and C(16:0). Based on the moderate levels of 16S rRNA gene sequence similarity to all members of the family Intrasporangiaceae and the unique combination of chemotaxonomic characteristics, strain IV-75(T) is considered to represent a novel species of a new genus, for which the name Aquipuribacter hungaricus gen. nov., sp. nov. is proposed. The type strain of Aquipuribacter hungaricus is IV-75(T) (=DSM 21674(T)=NCAIM B 02333(T)).
authors.affiliation: Eötvös Loránd University, Faculty of Science, Department of Microbiology, Pázmány Péter sétány 1/c, H-1117 Budapest, Hungary.
authors.forename: E M
authors.initials: EM
authors.lastname: Tóth
authors.affiliation: Eötvös Loránd University, Faculty of Science, Department of Microbiology, Pázmány Péter sétány 1/c, H-1117 Budapest, Hungary.
authors.forename: Zs
authors.initials: Z
authors.lastname: Kéki
authors.affiliation: Eötvös Loránd University, Faculty of Science, Department of Microbiology, Pázmány Péter sétány 1/c, H-1117 Budapest, Hungary.
authors.forename: V
authors.initials: V
authors.lastname: Bohus
authors.affiliation: Eötvös Loránd University, Faculty of Science, Department of Microbiology, Pázmány Péter sétány 1/c, H-1117 Budapest, Hungary.
authors.forename: A K
authors.initials: AK
authors.lastname: Borsodi
authors.affiliation: Eötvös Loránd University, Faculty of Science, Department of Microbiology, Pázmány Péter sétány 1/c, H-1117 Budapest, Hungary.
authors.forename: K
authors.initials: K
authors.lastname: Márialigeti
authors.affiliation: Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstrasse 7B, D-38124 Braunschweig, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Schumann
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003960:Diaminopimelic Acid / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D006814:Hungary; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011210:Power Plants; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000032:analysis; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D003960:Diaminopimelic Acid