title: Terminalia chebula extract acts as a potential NF-κB inhibitor in human lymphoblastic T cells.
abstract: Terminalia chebula (TC) is native to southern Asia to southwestern China and is used in traditional medicine for the treatment of human ailments including malignant tumors and diabetes. This plant also has antibacterial and immunomodulatory properties. Nuclear factor kappa-light chain-enhancer of activated B cells (NF-κB) is responsible for the expression of numerous genes involved in cell survival, proliferation, angiogenesis, inflammation, invasion and metastasis, among other processes. This study aims to assess the NF-κB inhibitory effect of TC extract in human lymphoblastic T (Jurkat) cells. The effects of TC extract were investigated using the FRET-based Gene Blazer technique in transfected Jurkat-NF-κB-RE-bla cells. The concentration of TC extract required for NF-κB inhibition was determined by a cell proliferation assay. Treatment with TC extract (50 μg/mL) inhibited NF-κB activity and protected against IκBα degradation and strongly suppressed IκBα phosphorylation in Jurkat-NF-κB-RE-bla cells. This treatment might be crucial for inhibiting NF-κB translocation and activation. In addition, the TC extract downregulated certain NF-κB regulated genes, including IL-8 and MCP-1, in Jurkat-NF-κB-RE-bla cells. Moreover, gallic acid was identified from the TC extract demonstrating its ability to inhibit NF-κB activity in Jurkat-NF-κB-RE-bla cells. Further studies to identify the role of gallic acid in NF-κB inhibition may uncover the crucial antiinflammatory and antitumor properties of the TC extract.
authors.affiliation: Division of Molecular and Life Science, Hanyang University, Ansan, 426-791, Korea.
authors.forename: Nando Dulal
authors.initials: ND
authors.lastname: Das
authors.forename: Kyoung Hwa
authors.initials: KH
authors.lastname: Jung
authors.forename: Ji Hyun
authors.initials: JH
authors.lastname: Park
authors.forename: Md Abdul Mojid
authors.initials: MA
authors.lastname: Mondol
authors.forename: Hee Jae
authors.initials: HJ
authors.lastname: Shin
authors.forename: Hyi-Seung
authors.initials: HS
authors.lastname: Lee
authors.forename: Kyoung Sun
authors.initials: KS
authors.lastname: Park
authors.forename: Mi Ran
authors.initials: MR
authors.lastname: Choi
authors.forename: Kyung Suk
authors.initials: KS
authors.lastname: Kim
authors.forename: Moo Sung
authors.initials: MS
authors.lastname: Kim
authors.forename: Sang Rin
authors.initials: SR
authors.lastname: Lee
authors.forename: Young Gyu
authors.initials: YG
authors.lastname: Chai
mesh_terms: D000818:Animals; D005707:Gallic Acid / Q000494:pharmacology*; D006801:Humans; D019169:Jurkat Cells; D051379:Mice; D016328:NF-kappa B / Q000037:antagonists & inhibitors* / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology; D013601:T-Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D029766:Terminalia / Q000737:chemistry*
chemical_list: D016328:NF-kappa B; D010936:Plant Extracts; D005707:Gallic Acid