title: Elevated serum 8-OHdG is associated with poor prognosis in epithelial ovarian cancer.
abstract: BACKGROUND
  8-Hydroxydeoxyguanosine (8-OHdG) is a marker of oxidative stress in DNA. This study was undertaken to reveal whether serum 8-OHdG could be a prognostic factor in epithelial ovarian carcinoma (EOC).
  
  
  METHODS
  Preoperative serum 8-OHdG levels were examined by enzyme-linked immunosorbent assay in 84 stage I-IV EOC patients. Immunohistochemical (IHC) 8-OHdG expression was determined in 78 of these patients.
  
  
  RESULTS
  Strong 8-OHdG immunostaining predicted poor survival. High serum 8-OHdG (>140 pg/ml) was associated with poor ovarian cancer-specific survival (p<0.05) in patients with grade 1-2 EOC (p<0.05), but was not observed among the grade 3 patients. High 8-OHdG levels both in the serum and in the tumour tissue was associated with traditional factors of poor prognosis and serous histology.
  
  
  CONCLUSIONS
  Both serum and IHC 8-OHdG assessment may serve as prognostic tools in EOC and the role of oxidative stress as a carcinogenic factor in ovarian cancer pathogenesis is also suggested.
authors.affiliation: Department of Obstetrics, University of Oulu, Finland.
authors.forename: Marjo
authors.initials: M
authors.lastname: Pylväs
authors.forename: Ulla
authors.initials: U
authors.lastname: Puistola
authors.forename: Liisa
authors.initials: L
authors.lastname: Laatio
authors.forename: Saila
authors.initials: S
authors.lastname: Kauppila
authors.forename: Peeter
authors.initials: P
authors.lastname: Karihtala
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D018262:Adenocarcinoma, Clear Cell / Q000097:blood* / Q000175:diagnosis / Q000601:surgery; D002288:Adenocarcinoma, Mucinous / Q000097:blood* / Q000175:diagnosis / Q000601:surgery; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000097:blood*; D018284:Cystadenocarcinoma, Serous / Q000097:blood* / Q000175:diagnosis / Q000601:surgery; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000097:blood; D016889:Endometrial Neoplasms / Q000097:blood* / Q000175:diagnosis / Q000601:surgery; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008875:Middle Aged; D009367:Neoplasm Staging; D010051:Ovarian Neoplasms / Q000097:blood* / Q000175:diagnosis / Q000601:surgery; D018384:Oxidative Stress; D011379:Prognosis; D015996:Survival Rate
chemical_list: D014408:Biomarkers, Tumor; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine