title: The conjunctival impression cytology between the diagnosed cases of dry eye and normal individuals.
abstract: BACKGROUND
  The dry eye or tear film dysfunction is a common ophthalmic syndrome.
  
  
  OBJECTIVE
  To compare the results of conjunctival impression cytology between dry eye patients and normal individuals.
  
  
  METHODS
  A case control study including consecutive cases of dry eye syndrome was carried out. Individuals without dry eye were taken as control. Impression of conjunctiva with cellulose acetate filter paper was taken from inferonasal bulbar conjunctiva and was stained with Periodic Acid- Schiff (PAS) and counter-stained with haematoxylin and eosin.
  
  
  METHODS
  goblet cell density.
  
  
  RESULTS
  There was a female preponderance in dry eye disease. Of 114 dry eye cases, 49.2% eyes showed decreased or absent goblet cell density. In 72 normal individuals 73.7% eyes showed normal goblet cell density and 26.3% of eyes showed decreased or absent goblet cells (p less than 0.001). The tear break-up time (TBUT) test was significantly more likely to be less than 10 seconds in cases as compared to the controls ( OR = 19.36, 95% CI = 7.56 - 52.52). Similarly, the goblet cell density was likely to be significantly reduced in cases with dry eye syndrome (OR= 2.25, 95% CI = 1.26 - 4.02, p = 0.003).
  
  
  CONCLUSIONS
  Goblet cell density significantly reduces in dry eye syndrome. The impression cytology is a useful test for the diagnosis of dry eye syndrome.
authors.affiliation: Consultant Ophthalmologist, Himalaya Eye Hospital, Pokhara, Nepal. eliyahshrestha@yahoo.com
authors.forename: E
authors.initials: E
authors.lastname: Shrestha
authors.forename: J K
authors.initials: JK
authors.lastname: Shrestha
authors.forename: G
authors.initials: G
authors.lastname: Shayami
authors.forename: M
authors.initials: M
authors.lastname: Chaudhary
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002452:Cell Count; D003228:Conjunctiva / Q000473:pathology*; D003584:Cytological Techniques / Q000379:methods; D003937:Diagnosis, Differential; D015352:Dry Eye Syndromes / Q000175:diagnosis*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results; D012189:Retrospective Studies; D013666:Tears / Q000166:cytology*; D055815:Young Adult