title: [Antitumor effects of matrix protein of vesicular stomatic virus on EL4 lymphoma mice].
abstract: OBJECTIVE
  To explore antitumor effects of plasmid pcDNA3. 1-MP encoding matrix protein of vesicular stomatitis virus (VSV) complexed with cationic liposome (DOTAP:CHOL) in mice with EL4 lymphoma.
  
  
  METHODS
  C57BL/6 mouse model with EL4 lymphoma was established. Sixty mice bearing EL4 lymphoma were divided randomly into five groups including Lip-MP, Lip-pVAX, Lip, ADM and NS groups, which were intravenously injected with liposome-pcDNA 3. 1-MP complex, liposome-pVAX complex, empty liposome, Adriamycin and normal saline respectively every three days. Tumor volumes and survival time were monitored. Microvessel density and tumor proliferative index in tumor tissues were determined by CD31, Ki-67 immunohistochemistry staining, meanwhile the tumor apoptosis index was measured by TUNEL method.
  
  
  RESULTS
  From 6 days after treatments on, the tumor volume in Lip-MP group was much smaller than that in Lip-pVAX, Lip and NS group (P < 0.05). The median survival time of mice in Lip-MP group, 44 days after inoculation of tumor cells, was significantly higher than that in other groups (P < 0.05), which was 39 days, 38.5 days and 34 days in Lip-pVAX, Lip and NS groups respectively. The MVD value in tumor tissues in Lip-MP group was less than that in Lip-pVAX, Lip and NS groups (P < 0.05). Ki67 staining revealed that Lip-MP complex apparently suppressed the proliferation of EL4 tumor cells in vivo (P < 0.05). TUNEL assays showed that apoptosis index of tumor cells in Lip-MP group, 10.60 +/- 1.71, was much higher than that in other three groups (P < 0.05).
  
  
  CONCLUSIONS
  Lip-MP complex, the plasmid encoding matrix protein of VSV (VSV-MP) encapsulated in cationic liposome, significantly inhibited the growth of tumor and prolonged the survival of mice bearing EL4 lymphoma, which may be related to the induction of tumor cell apoptosis, inhibition of tumor angiogenesis, and suppression of tumor cell proliferation.
authors.affiliation: Department of Hematology & Research Laboratory of Hematology, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Shi-jia
authors.initials: SJ
authors.lastname: Lin
authors.forename: Qin-mei
authors.initials: QM
authors.lastname: Yu
authors.forename: Wen-tong
authors.initials: WT
authors.lastname: Meng
authors.forename: Yan-jun
authors.initials: YJ
authors.lastname: Wen
authors.forename: Li-juan
authors.initials: LJ
authors.lastname: Chen
authors.forename: Ting
authors.initials: T
authors.lastname: Niu
mesh_terms: D000818:Animals; D015316:Genetic Therapy / Q000379:methods; D008081:Liposomes / Q000008:administration & dosage; D008223:Lymphoma / Q000628:therapy*; D051379:Mice; D008810:Mice, Inbred C57BL; D010957:Plasmids; D011897:Random Allocation; D011994:Recombinant Proteins / Q000235:genetics / Q000494:pharmacology; D018116:Vesiculovirus / Q000378:metabolism; D014763:Viral Matrix Proteins / Q000235:genetics / Q000494:pharmacology*
chemical_list: D008081:Liposomes; C094460:M protein, Vesicular stomatitis virus; D011994:Recombinant Proteins; D014763:Viral Matrix Proteins