title: [Functional activity of monocytes and mechanisms of iNOS intracellular regulation during wound process].
abstract: To investigate the dynamics and mechanisms of iNOS activity during wound process, the peripheral blood monocytes of 22 patients with acute foot wounds were analyzed. The basal and LPS-stimulated nitrites production was estimated at 1, 3-4, 7-10 and 14-18 days of wound process. iNOS activity and its molecular regulation was estimated by the inhibitory analysis. It was shown that since 1 to 3-4 day of wound process the basal and LPS-stimulated activity of iNOS, PDE and 5-LOX were elevated initially and than decreased. The COX and PkC activities increased after 3 days and reached the maximum level at days 7-10. The activity of PkA, which inhibits iNOS, intensively increased form 7-10th to 14-18th days of healing, and was accompanied by arginase-1 stimulation. Thus monitoring of intracellular signaling system can be used for diagnostics and correction of wound healing.
authors.forename: M E
authors.initials: ME
authors.lastname: Barinova
authors.forename: V M
authors.initials: VM
authors.lastname: Iel's'kyĭ
authors.forename: E F
authors.initials: EF
authors.lastname: Barinov
authors.forename: O M
authors.initials: OM
authors.lastname: Sulaieva
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D002478:Cells, Cultured; D005121:Extremities / Q000293:injuries*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000201:enzymology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008875:Middle Aged; D009569:Nitric Oxide / Q000096:biosynthesis / Q000502:physiology; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism* / Q000502:physiology; D013997:Time Factors; D014945:Wound Healing / Q000502:physiology; D014950:Wounds, Penetrating / Q000097:blood* / Q000201:enzymology
chemical_list: D008070:Lipopolysaccharides; D009569:Nitric Oxide; C496319:NOS2 protein, human; D052247:Nitric Oxide Synthase Type II