title: Resolvin D1 protects mice from LPS-induced acute lung injury.
abstract: Resolvin D1 (RvD1), an endogenous lipid molecule derived from docosahexaenoic acid (DHA), has been described to promote inflammatory resolution. The present study aimed to determine the protective effects and the underlying mechanisms of RvD1 on lipopolysaccharide (LPS)-induced acute lung injury (ALI). Pretreatment RvD1 to mice 30 min before inducing ALI by LPS decreased the mortality and improved lung pathological changes, inhibited LPS-induced increases in polymorphonulear and mononuclear leukocytes recruitment, total proteins content, tumor necrosis factor (TNF-α) and interleukin-6 (IL-6) production in the bronchoalveolar lavage fluids (BALFs). In addition, RvD1 markedly reduced LPS-induced the expression of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), and adhesion molecules, as well as myeloperoxidase (MPO) activity. Moreover, RvD1 markedly inhibited LPS-induced the activation of mitogen-activated protein kinases (MAPKs) and nuclear factor-κB (NF-κB). Furthermore, pretreatment with Boc, a lipoxin A4 receptor (ALX) antagonist, significantly reversed these beneficial effects of RvD1 on LPS-induced acute lung injury in mice. Taken together, our study showed that RvD1 improved survival rate and attenuated ALI in mice induced by LPS, and the protective mechanisms might be related to selective reaction with ALX, which inhibits MAPKs and NF-κB pathway.
authors.affiliation: Department of Anesthesiology, The First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.forename: Xia
authors.initials: X
authors.lastname: Gong
authors.forename: Jing-yuan
authors.initials: JY
authors.lastname: Wan
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Zhang
authors.forename: Hong-zhong
authors.initials: HZ
authors.lastname: Li
authors.forename: Su
authors.initials: S
authors.lastname: Min
mesh_terms: D055371:Acute Lung Injury / Q000201:enzymology / Q000209:etiology / Q000517:prevention & control*; D000818:Animals; D015815:Cell Adhesion Molecules / Q000032:analysis; D015232:Dinoprostone / Q000032:analysis; D004281:Docosahexaenoic Acids / Q000627:therapeutic use*; D007958:Leukocyte Count; D008070:Lipopolysaccharides / Q000633:toxicity*; D008168:Lung / Q000187:drug effects / Q000473:pathology; D020935:MAP Kinase Signaling System / Q000187:drug effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000037:antagonists & inhibitors; D052247:Nitric Oxide Synthase Type II / Q000032:analysis; D009195:Peroxidase / Q000378:metabolism
chemical_list: D015815:Cell Adhesion Molecules; D008070:Lipopolysaccharides; D016328:NF-kappa B; C518399:resolvin D1; D004281:Docosahexaenoic Acids; D009195:Peroxidase; D052247:Nitric Oxide Synthase Type II; D015232:Dinoprostone