title: Do commercial serologic tests for Trypanosoma cruzi infection detect Mexican strains in women and newborns?
abstract: We sought to determine the serological test that could be used for Trypanosoma cruzi seroprevalence studies in Mexico, where lineage I predominates. In a previous study among pregnant women and their newborns in the states of Yucatan and Guanajuato, we reported a 0.8-0.9% of prevalence for T. cruzi -specific antibodies by Stat-Pak and Wiener ELISA. We have expanded this study here by performing an additional non-commercial ELISA and confirming the seropositives with Western blot, using whole antigens of a local parasite strain. We found a seroprevalence of 0.6% (3/500) in Merida and 0.4% in Guanajuato (2/488). The 5 seropositive umbilical cord samples reacted to both non-commercial ELISA and Western blot tests, and only 1 of the maternal samples was not reactive to non-commercial ELISA. A follow-up of the newborns at 10 mo was performed in Yucatan to determine the presence of T. cruzi antibodies in children as evidence of congenital infection. None of the children was seropositive. One newborn from an infected mother died at 2 wk of age of cardiac arrest, but T. cruzi infection was not confirmed. The T. cruzi seroprevalence data obtained with both commercial tests (Stat-Pak and ELISA Wiener) are similar to those from non-commercial tests using a local Mexican strain of T. cruzi.
authors.affiliation: Laboratorio de Parasitología, Centro de Investigaciones Regionales "Dr. Hideyo Noguchi", Universidad Autónoma de Yucatán, Ave. Itzaes 490 x 59A, 97000 Mérida, Yucatán, México. gleon@uady.mx
authors.forename: Rubi
authors.initials: R
authors.lastname: Gamboa-León
authors.forename: Claudia
authors.initials: C
authors.lastname: Gonzalez-Ramirez
authors.forename: Nicolas
authors.initials: N
authors.lastname: Padilla-Raygoza
authors.forename: Sergio
authors.initials: S
authors.lastname: Sosa-Estani
authors.forename: Alejandra
authors.initials: A
authors.lastname: Caamal-Kantun
authors.forename: Pierre
authors.initials: P
authors.lastname: Buekens
authors.forename: Eric
authors.initials: E
authors.lastname: Dumonteil
mesh_terms: D000328:Adult; D000913:Antibodies, Protozoan / Q000097:blood*; D000953:Antigens, Protozoan / Q000276:immunology; D015153:Blotting, Western; D014355:Chagas Disease / Q000175:diagnosis* / Q000453:epidemiology / Q000469:parasitology / Q000635:transmission; D003430:Cross-Sectional Studies; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000592:standards; D005260:Female; D005312:Fetal Blood / Q000276:immunology; D006801:Humans; D007231:Infant, Newborn; D018445:Infectious Disease Transmission, Vertical; D008800:Mexico / Q000453:epidemiology; D011247:Pregnancy; D015597:Pregnancy Complications, Parasitic / Q000175:diagnosis* / Q000453:epidemiology / Q000469:parasitology; D012680:Sensitivity and Specificity; D016036:Seroepidemiologic Studies; D014349:Trypanosoma cruzi / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification; D055815:Young Adult
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan