title: Interaction between drug loaded polyaspartamide-polylactide-polysorbate based micelles and cell membrane models: a calorimetric study.
abstract: Amphiphilic biodegradable copolymers, for their ability to self-assemble into micelle-like aggregates, with a suitable loading capacity, are of emerging interest for the delivery of water-insoluble drugs. α,β-Poly[(N-hydroxyethyl)-dl-aspartamide] (PHEA) is suitable to obtain amphiphilic graft copolymers. These copolymers can be obtained starting from PHEA-ethylenediamine (PHEA-EDA) which is functionalized with polysorbate 80 (PS₈₀, like targeting residues to the brain) and polylactide (PLA, like hydrophobic chains) in order to obtain polymeric micelles of PHEA-EDA-PS₈₀-PLA potentially useful to release drugs to the central nervous system. In this paper, the interaction and absorption of PHEA-EDA-PS₈₀-PLA micelles loaded with (R)-flurbiprofen with biomembrane models, represented by multilamellar or unilamellar vesicles made of dimyristoylphosphatidylcholine, are investigated by means of differential scanning calorimetry technique. (R)-Flurbiprofen is the single enantiomer of the racemate flurbiprofen; the capacity of this nonsteroidal anti-inflammatory drug to reduce risk of Alzheimer's disease has been recently reported. Drug release from the micelles to the lipid vesicles has been investigated in simulated physiological fluid, and it resulted to be affected by the biomembrane model.
authors.affiliation: Dipartimento di Scienze del Farmaco, Università degli Studi di Catania , Viale A. Doria 6, 95125 Catania, Italy.
authors.forename: Maria Grazia
authors.initials: MG
authors.lastname: Sarpietro
authors.forename: Giovanna
authors.initials: G
authors.lastname: Pitarresi
authors.forename: Sara
authors.initials: S
authors.lastname: Ottimo
authors.forename: Maria Chiara
authors.initials: MC
authors.lastname: Giuffrida
authors.forename: Maria Chiara
authors.initials: MC
authors.lastname: Ognibene
authors.forename: Calogero
authors.initials: C
authors.lastname: Fiorica
authors.forename: Gaetano
authors.initials: G
authors.lastname: Giammona
authors.forename: Francesco
authors.initials: F
authors.lastname: Castelli
mesh_terms: D002152:Calorimetry, Differential Scanning; D002462:Cell Membrane; D005480:Flurbiprofen / Q000737:chemistry; D007700:Kinetics; D008081:Liposomes / Q000737:chemistry; D008823:Micelles; D011091:Polyesters / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011136:Polysorbates / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D008081:Liposomes; D008823:Micelles; D011091:Polyesters; D011108:Polymers; D011136:Polysorbates; C033616:poly(lactide); D005480:Flurbiprofen