title: Plasma cytokine response, lipid peroxidation and NF-kB activation in skeletal muscle following maximum progressive swimming.
abstract: Our objective was to determine lipid peroxidation and nuclear factor-κB (NF-κB) activation in skeletal muscle and the plasma cytokine profile following maximum progressive swimming. Adult male Swiss mice (N = 15) adapted to the aquatic environment were randomly divided into three groups: immediately after exercise (EX1), 3 h after exercise (EX2) and control. Animals from the exercising groups swam until exhaustion, with an initial workload of 2% of body mass attached to the tail. Control mice did not perform any exercise but were kept immersed in water for 20 min. Maximum swimming led to reactive oxygen species (ROS) generation in skeletal muscle, as indicated by increased thiobarbituric acid reactive species (TBARS) levels (4062.67 ± 1487.10 vs 19,072.48 ± 8738.16 nmol malondialdehyde (MDA)/mg protein, control vs EX1). Exercise also promoted NF-κB activation in soleus muscle. Cytokine secretion following exercise was marked by increased plasma interleukin-6 (IL-6) levels 3 h post-exercise (P < 0.05). Interleukin-10 (IL-10) levels were reduced following exercise and remained reduced 3 h post-exercise (P < 0.05). Plasma levels of other cytokines investigated, monocyte chemotactic protein-1 (MCP-1), tumor necrosis factor-alpha (TNF-α), interferon-gamma (IFN-γ) and interleukin-12 (IL-12), were not altered by exercise. The present findings showed that maximum swimming, as well as other exercise models, led to lipid peroxidation and NF-κB activation in skeletal muscle and increased plasma IL-6 levels. The plasma cytokine response was also marked by reduced IL-10 levels. These results were attributed to exercise type and intensity.
authors.affiliation: Departamento de Ciências Biológicas, Ambientais e da Saúde, Centro Universitário de Belo Horizonte, Belo Horizonte, MG, Brazil.
authors.forename: L S
authors.initials: LS
authors.lastname: Cleto
authors.forename: A F
authors.initials: AF
authors.lastname: Oleto
authors.forename: L P
authors.initials: LP
authors.lastname: Sousa
authors.forename: T O
authors.initials: TO
authors.lastname: Barreto
authors.forename: J S
authors.initials: JS
authors.lastname: Cruz
authors.forename: C L
authors.initials: CL
authors.lastname: Penaforte
authors.forename: J C
authors.initials: JC
authors.lastname: Magalhães
authors.forename: J
authors.initials: J
authors.lastname: Sousa-Franco
authors.forename: K M C
authors.initials: KM
authors.lastname: Pinto
authors.forename: A C
authors.initials: AC
authors.lastname: Campi-Azevedo
authors.forename: E
authors.initials: E
authors.lastname: Rocha-Vieira
mesh_terms: D000818:Animals; D015992:Body Mass Index; D016207:Cytokines / Q000097:blood*; D016753:Interleukin-10 / Q000097:blood; D018664:Interleukin-12 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D015227:Lipid Peroxidation / Q000502:physiology*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D018482:Muscle, Skeletal / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism*; D010805:Physical Conditioning, Animal / Q000502:physiology; D011897:Random Allocation; D017382:Reactive Oxygen Species / Q000378:metabolism; D013550:Swimming / Q000502:physiology*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D013997:Time Factors
chemical_list: D016207:Cytokines; D015850:Interleukin-6; D016328:NF-kappa B; D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances; D016753:Interleukin-10; D018664:Interleukin-12; D008315:Malondialdehyde