title: Structural elements of the signal propagation pathway in squid rhodopsin and bovine rhodopsin.
abstract: Squid and bovine rhodopsins are G-protein coupled receptors (GPCRs) that activate Gq- and Gt-type G-proteins, respectively. To understand the structural elements of the signal propagation pathway, we performed molecular dynamics (MD) simulations of squid and bovine rhodopsins plus a detailed sequence analysis of class A GPCRs. The computations indicate that although the geometry of the retinal is similar in bovine and squid rhodopsins, the important interhelical hydrogen bond networks are different. In squid rhodopsin, an extended hydrogen bond network that spans ∼13 Å to Tyr315 on the cytoplasmic site is present regardless of the protonation state of Asp80. In contrast, the extended hydrogen bond network is interrupted at Tyr306 in bovine rhodopsin. Those differences in the hydrogen bond network may play significant functional roles in the signal propagation from the retinal binding site to the cytoplasmic site, including transmembrane helix (TM) 6 to which the G-protein binds. The MD calculations demonstrate that the elongated conformation of TM6 in squid rhodopsin is stabilized by salt bridges formed with helix (H) 9. Together with the interhelical hydrogen bonds, the salt bridges between TM6 and H9 stabilize the protein conformation of squid rhodopsin and may hinder the occurrence of large conformational changes that are observed upon activation of bovine rhodopsin.
authors.affiliation: Computational Biology Research Center (CBRC), National Institute of Advanced Industrial Science and Technology (AIST), AIST Tokyo Waterfront BIO-IT Research Building, 2-4-7 Aomi, Tokyo 135-0064, Japan.
authors.forename: Minoru
authors.initials: M
authors.lastname: Sugihara
authors.forename: Wataru
authors.initials: W
authors.lastname: Fujibuchi
authors.forename: Makiko
authors.initials: M
authors.lastname: Suwa
mesh_terms: D000818:Animals; D002417:Cattle; D049832:Decapodiformes / Q000378:metabolism*; D006860:Hydrogen Bonding; D056004:Molecular Dynamics Simulation; D011789:Quantum Theory; D012243:Rhodopsin / Q000737:chemistry*; D015398:Signal Transduction
chemical_list: D012243:Rhodopsin