title: Crystallization and preliminary X-ray diffraction analysis of a galactose-specific lectin from the seeds of Butea monosperma.
abstract: The galactose-specific lectin from the seeds of Butea monosperma has been crystallized by the hanging-drop vapour-diffusion technique. The crystals belonged to space group P1, with unit-cell parameters a = 78.45, b = 78.91, c = 101.85 Å, α = 74.30, β = 76.65, γ = 86.88°. X-ray diffraction data were collected to a resolution of 2.44 Å under cryoconditions (100 K) using a MAR image-plate detector system mounted on a rotating-anode X-ray generator. Molecular-replacement calculations carried out using the coordinates of several structures of legume lectins as search models indicate that the galactose-specific lectin from B. monosperma forms an octamer.
authors.affiliation: Department of Biotechnology and Microbiology, Kannur University, Kannur, Kerala 670 661, India.
authors.forename: Joseph
authors.initials: J
authors.lastname: Abhilash
authors.forename: K
authors.initials: K
authors.lastname: Geethanandan
authors.forename: S R
authors.initials: SR
authors.lastname: Bharath
authors.forename: C
authors.initials: C
authors.lastname: Sadasivan
authors.forename: M
authors.initials: M
authors.lastname: Haridas
mesh_terms: D031290:Butea / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D005690:Galactose / Q000378:metabolism; D037102:Lectins / Q000737:chemistry* / Q000378:metabolism; D012639:Seeds / Q000737:chemistry
chemical_list: D037102:Lectins; D005690:Galactose