title: Myostatin regulates preadipocyte differentiation and lipid metabolism of adipocyte via ERK1/2.
abstract: Myostatin is known as an inhibitor of muscle development, but its role in adipogenesis and lipid metabolism is still unclear, especially the underlying mechanisms. Here, we demonstrated that myostatin inhibited 3T3-L1 preadipocyte differentiation into adipocyte by suppressing C/EBPα (CCAAT/enhancer-binding protein α) and PPARγ (peroxisome-proliferator-activated receptor γ), also activated ERK1/2 (extracellular-signal-regulated kinase 1/2). Furthermore, myostatin enhanced the phosphorylation of HSL (hormone-sensitive lipase) and ACC (acetyl-CoA carboxylase) in fully differentiated adipocytes, as well as ERK1/2. Besides, we noted that myostatin markedly raised the levels of leptin and adiponectin release and mRNA expression during preadipocyte differentiation, but the levels were inhibited by myostatin treatments in fully differentiated adipocytes. These results suggested that myostatin suppressed 3T3-L1 preadipocyte differentiation and regulated lipid metabolism of mature adipocyte, in part, via activation of ERK1/2 signalling pathway.
authors.affiliation: Laboratory of Agroecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan 410125, Peoples Republic of China.
authors.forename: Fengna
authors.initials: F
authors.lastname: Li
authors.forename: Huansheng
authors.initials: H
authors.lastname: Yang
authors.forename: Yehui
authors.initials: Y
authors.lastname: Duan
authors.forename: Yulong
authors.initials: Y
authors.lastname: Yin
mesh_terms: D016475:3T3 Cells; D000103:Acetyl-CoA Carboxylase / Q000378:metabolism; D017667:Adipocytes / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D050156:Adipogenesis / Q000187:drug effects*; D054392:Adipokines / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D000818:Animals; D022762:CCAAT-Enhancer-Binding Proteins / Q000378:metabolism; D005786:Gene Expression Regulation; D050356:Lipid Metabolism; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D051379:Mice; D055435:Myostatin / Q000494:pharmacology*; D047495:PPAR gamma / Q000378:metabolism; D010766:Phosphorylation; D012333:RNA, Messenger / Q000032:analysis; D044967:Serum / Q000378:metabolism; D002787:Sterol Esterase / Q000378:metabolism; D013997:Time Factors
chemical_list: D054392:Adipokines; D022762:CCAAT-Enhancer-Binding Proteins; C530718:CEBPA protein, mouse; D055435:Myostatin; D047495:PPAR gamma; D012333:RNA, Messenger; D002787:Sterol Esterase; D000103:Acetyl-CoA Carboxylase