title: Description of microsatellite markers and genotyping performances using feathers and buccal swabs for the Ivory gull (Pagophila eburnea).
abstract: We report 22 new polymorphic microsatellites for the Ivory gull (Pagophila eburnea), and we describe how they can be efficiently co-amplified using multiplexed polymerase chain reactions. In addition, we report DNA concentration, amplification success, rates of genotyping errors and the number of genotyping repetitions required to obtain reliable data with three types of noninvasive or nondestructive samples: shed feathers collected in colonies, feathers plucked from living individuals and buccal swabs. In two populations from Greenland (n=21) and Russia (Severnaya Zemlya Archipelago, n=21), the number of alleles per locus varied between 2 and 17, and expected heterozygosity per population ranged from 0.18 to 0.92. Twenty of the markers conformed to Hardy-Weinberg and linkage equilibrium expectations. Most markers were easily amplified and highly reliable when analysed from buccal swabs and plucked feathers, showing that buccal swabbing is a very efficient approach allowing good quality DNA retrieval. Although DNA amplification success using single shed feathers was generally high, the genotypes obtained from this type of samples were prone to error and thus need to be amplified several times. The set of microsatellite markers described here together with multiplex amplification conditions and genotyping error rates will be useful for population genetic studies of the Ivory gull.
authors.affiliation: Department of Ecology and Evolution, University of Lausanne, CH-1015 Lausanne, Switzerland. Glenn.Yannic@gmail.com
authors.forename: Glenn
authors.initials: G
authors.lastname: Yannic
authors.forename: Roberto
authors.initials: R
authors.lastname: Sermier
authors.forename: Adrian
authors.initials: A
authors.lastname: Aebischer
authors.forename: Maria V
authors.initials: MV
authors.lastname: Gavrilo
authors.forename: Olivier
authors.initials: O
authors.lastname: Gilg
authors.forename: Cecilie
authors.initials: C
authors.lastname: Miljeteig
authors.forename: Brigitte
authors.initials: B
authors.lastname: Sabard
authors.forename: Hallvard
authors.initials: H
authors.lastname: Strøm
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Pouivé
authors.forename: T
authors.initials: T
authors.lastname: Broquet
mesh_terms: D000483:Alleles; D000818:Animals; D046091:Charadriiformes / Q000235:genetics*; D005241:Feathers / Q000737:chemistry*; D014644:Genetic Variation; D005838:Genotype; D006116:Greenland; D015810:Linkage Disequilibrium; D018895:Microsatellite Repeats / Q000235:genetics*; D009061:Mouth Mucosa / Q000737:chemistry*; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D012426:Russia