title: [Topoisomerase IIalpha activity and tumor cell proliferation level from the degree of bromodeoxyuridine label in uterine body leiomyosarcoma].
abstract: The investigation was undertaken to estimate the expression of the enzyme gyrase Topoisomerase IIalpha (TOPOIIalpha) and proliferative activity from the level of bromodeoxyuridine (Brdu) in uterine body leiomyosarcoma (UBL) (n=66) versus the unchanged myometrium (UM), a tumor of uncertain malignancy potential (STUMP) (n=12), and leiomyoma (LM) (n=46). An immunohistochemical technique was used to reveal TopOIIalpha and Brdu. The findings indicated that Brdu was undetectable in UM and simple LM and that TOPOIIalpha activity was low. At the same time there was an exponential rise in the activity of the enzyme and cells during the S phase of the cell cycle from cellular LM to UBL. An increase was also noted in the study markers as sarcoma malignancy progressed. Thus, it can be said that the estimation of the number of cell S fractions and the activity of TOPOIIalpha in UM, LM, STUMP, and UBL may serve as a relatively simple and reliable method for the differential diagnosis of benign and malignant smooth muscle tumors of the uterine body and the determination of the activity of the enzyme may be used to define the degree of tumor chemoresistance in the complex treatment of patients with UBL.
authors.forename: A F
authors.initials: AF
authors.lastname: Lazarev
authors.forename: A M
authors.initials: AM
authors.lastname: Avdalian
authors.forename: I P
authors.initials: IP
authors.lastname: Bobrov
authors.forename: V V
authors.initials: VV
authors.lastname: Klimachev
mesh_terms: D000328:Adult; D000951:Antigens, Neoplasm / Q000378:metabolism*; D001973:Bromodeoxyuridine / Q000737:chemistry; D004250:DNA Topoisomerases, Type II / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D007890:Leiomyosarcoma / Q000201:enzymology* / Q000473:pathology*; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000378:metabolism*; D016196:S Phase; D013194:Staining and Labeling / Q000379:methods; D014594:Uterine Neoplasms / Q000201:enzymology* / Q000473:pathology*
chemical_list: D000951:Antigens, Neoplasm; D004268:DNA-Binding Proteins; D009363:Neoplasm Proteins; D004250:DNA Topoisomerases, Type II; D001973:Bromodeoxyuridine