title: Identification of unknown ocular pathogens in clinically suspected eye infections using ribosomal RNA gene sequence analysis.
abstract: DNA sequence-based identification of pathogens from ocular samples of patients with clinically suspected eye infections was accomplished using 16S and internal transcribed spacer (ITS) ribosomal RNA gene sequence analysis. PCR was positive for 24 of 99 samples tested. Both culture and 16S rDNA sequence analysis identified Pseudomonas aeruginosa, streptococci and Enterobacteriaceae. Isolates misidentified as Burkholderia cepacia by biochemical tests were identified as Ralstonia mannitolilytica by 16S rDNA sequence analysis. Sequence analysis identified the following microorganisms from 19 culture-negative samples: Haemophilus influenzae, Sphingomonas sp., Klebsiella pneumoniae, Staphylococcus haemolyticus, Morganella morganii, Mycobacterium sp., Chryseobacterium sp., Pseudomonas saccharophila (Xanthomonas) and the fungus, Phaeoacremonium inflatipes.
authors.affiliation: Research and Biotechnology Division, St. Luke's Medical Centre, Quezon City, Philippines. mldaroy@yahoo.com
authors.forename: M L G
authors.initials: ML
authors.lastname: Daroy
authors.forename: J S
authors.initials: JS
authors.lastname: Lopez
authors.forename: B C L
authors.initials: BC
authors.lastname: Torres
authors.forename: M J
authors.initials: MJ
authors.lastname: Loy
authors.forename: P M C
authors.initials: PM
authors.lastname: Tuaño
authors.forename: R R
authors.initials: RR
authors.lastname: Matias
mesh_terms: D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D015818:Eye Infections, Bacterial / Q000175:diagnosis*; D015821:Eye Infections, Fungal / Q000175:diagnosis*; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D021903:DNA, Ribosomal Spacer; D012336:RNA, Ribosomal, 16S