title: Subcellular features revealed on unfixed rat brain sections by phase imaging.
abstract: For sectioned biologic tissues, atomic force microscopy (AFM) topographic images alone hardly provide adequate information leading to revealing biological structures. We demonstrate that phase imaging in amplitude-modulation AFM is a powerful tool in mapping structures present on the surface of unfixed rat brains sections. The contrast in phase images is originated from the difference in mechanical properties between biological structures. Visualization of the native state of biological structures by way of their mechanical properties provides a complementary technique to more traditional imaging techniques such as optical and electron microscopy.
authors.affiliation: Surface Science Western, The University of Western Ontario, 999 Collip Circle, London, Ontario N6G 0J3, Canada. hnie@uwo.ca
authors.forename: H-Y
authors.initials: HY
authors.lastname: Nie
authors.forename: A R
authors.initials: AR
authors.lastname: Taylor
authors.forename: W M
authors.initials: WM
authors.lastname: Lau
authors.forename: D F
authors.initials: DF
authors.lastname: MacFabe
mesh_terms: D000818:Animals; D001921:Brain / Q000033:anatomy & histology / Q000648:ultrastructure*; D008055:Lipids / Q000032:analysis; D008297:Male; D018625:Microscopy, Atomic Force / Q000295:instrumentation / Q000379:methods*; D051381:Rats; D020318:Rats, Long-Evans; D018629:Spectrometry, Mass, Secondary Ion
chemical_list: D008055:Lipids