title: Use of a novel microfluidic disk in the analysis of single-cell viability and the application to Jurkat cells.
abstract: Jurkat cells were trapped in the microchambers of a novel disk-shaped cell separation device and stained with Cellstain. Approximately 90% of the cells were living. Single cells were isolated with a branching microchannel after rotation at 4500rpm for 30s, demonstrating that a living single cell could be trapped in the microchambers.
authors.affiliation: Department of Bioinformatics, Graduate School of Engineering, Soka University, Hachioji, Tokyo 192-8577, Japan. kubo@t.soka.ac.jp
authors.forename: Izumi
authors.initials: I
authors.lastname: Kubo
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Furutani
authors.forename: Kenji
authors.initials: K
authors.lastname: Matoba
mesh_terms: D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D002470:Cell Survival; D006801:Humans; D019169:Jurkat Cells; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D044085:Microfluidics