title: [Detection and significance of CD4+ CD25int/high CD127low regulatory T cells in pediatric aplastic anemia].
abstract: OBJECTIVE
  To study the expression of CD4+ CD25int/high CD127low regulatory T cells in peripheral blood (PB) and its relation to the quantity of Hb, WBC and platelet (Plt) in children with aplastic anemia (AA).
  
  
  METHODS
  Expression of CD4+ CD25int/high CD127low in PB was detected by flow cytometry in 22 children with AA before and after treatment and in 15 healthy controls. The relationships between CD4+CD25highCD127low and the quantity of Hb, WBC and Plt were evaluated.
  
  
  RESULTS
  Compared to controls, the percentages of CD4+ CD25+/CD4+, CD4+CD25high/CD4+, CD4+ CD25+ CD127low/CD4+ and CD4+CD25highCD127low/CD4+ in PB of AA patients decreased markedly at the active phase (P﹤0.05). By the recovery phase, the percentages of CD4+CD25+/CD4+, CD4+CD25high/CD4+, CD4+ CD25+ CD127low/CD4+ and CD4+CD25highCD127low/CD4+ increased significantly to the levels similar to the controls. There were significant positive relationships between the expression of CD4+CD25highCD127low cells and the quantity of Hb, WBC and Plt (r=0.499, 0.526, 0.540 respectively; P﹤0.05).
  
  
  CONCLUSIONS
  The decrease of the percentage of CD4+CD25int/highCD127low regulatory T cells might be associated with the development of pediatric AA. The CD4+CD25int/highCD127low regulatory T cells can serve as a marker for the evaluation of disease severity as well as a target of further study on immune treatment of AA.
authors.affiliation: Department of Pediatrics, Third Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China. xigewang1962@sina.com
authors.forename: Xi-Ge
authors.initials: XG
authors.lastname: Wang
authors.forename: Yi-Ming
authors.initials: YM
authors.lastname: Cao
authors.forename: Xiao-Ge
authors.initials: XG
authors.lastname: Wang
mesh_terms: D000293:Adolescent; D000741:Anemia, Aplastic / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D053699:Interleukin-7 Receptor alpha Subunit / Q000032:analysis*; D008297:Male; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
chemical_list: D053699:Interleukin-7 Receptor alpha Subunit