title: Comparative study of two in vitro methods for assessing drug absorption: Sartorius SM 16750 apparatus versus Everted Gut Sac.
abstract: OBJECTIVE
  Oral drug administration remains the most common and most convenient way used in clinical therapy. The availability of a simple, rapid, economic and reproducible in vitro method to assess the rate, extent and mechanism of intestinal drug absorption is a very helpful tool. The purpose of this study was to compare the performance of Sartorius SM 16750 Absorption Simulator apparatus to Everted Gut Sac (EGS) technique in terms of predicting drug permeability.
  
  
  METHODS
  Permeation studies across these two in vitro models were performed with six drugs selected across the Biopharmaceutics Classification System (BCS) categories: tramadol (class I of BCS), doxycycline (class I of BCS), diclofenac (class II of BCS), clopidogrel (class II of BCS), metformin (class III of BCS) and chlorothiazide (class IV of BCS).
  
  
  RESULTS
  Apparent permeability coefficient (Papp) and diffusion profiles obtained with EGS and Sartorius SM 16750 apparatus were similar for diclofenac and metformin, whereas, we noticed significant differences (p ≤ 0.05), for tramadol, doxycycline, clopidogrel and chlorothiazide.
  
  
  CONCLUSIONS
  Compared to Everted Gut Sac model, Sartorius SM 16750 absorption simulator apparatus seems to have limited application for the assessment of intestinal drug absorption since it does not take into consideration the involvement of others processes than the passive transcellular pathway as mechanism of drug absorption.
authors.affiliation: Laboratory of Galenic Pharmacy, Faculty of Pharmacy of Monastir, University of Monastir, 5000 Monastir, Tunisia. lassoued98@yahoo.fr
authors.forename: Mohamed Ali
authors.initials: MA
authors.lastname: Lassoued
authors.forename: Fathia
authors.initials: F
authors.lastname: Khemiss
authors.forename: Souad
authors.initials: S
authors.lastname: Sfar
mesh_terms: D000284:Administration, Oral; D000818:Animals; D007408:Intestinal Absorption; D008297:Male; D008954:Models, Biological; D010539:Permeability; D004364:Pharmaceutical Preparations / Q000737:chemistry / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012995:Solubility
chemical_list: D004364:Pharmaceutical Preparations