title: MiR-21 plays an important role in radiation induced carcinogenesis in BALB/c mice by directly targeting the tumor suppressor gene Big-h3.
abstract: Dysregulation of certain microRNAs (miRNAs) in cancer can promote tumorigenesis, metastasis and invasion. However, the functions and targets of only a few mammalian miRNAs are known. In particular, the miRNAs that participates in radiation induced carcinogenesis and the miRNAs that target the tumor suppressor gene Big-h3 remain undefined. Here in this study, using a radiation induced thymic lymphoma model in BALB/c mice, we found that the tumor suppressor gene Big-h3 is down-regulated and miR-21 is up-regulated in radiation induced thymic lymphoma tissue samples. We also found inverse correlations between Big-h3 protein and miR-21 expression level among different tissue samples. Furthermore, our data indicated that miR-21 could directly target Big-h3 in a 3'UTR dependent manner. Finally, we found that miR-21 could be induced by TGFβ, and miR-21 has both positive and negative effects in regulating TGFβ signaling. We conclude that miR-21 participates in radiation induced carcinogenesis and it regulates TGFβ signaling.
authors.affiliation: Department of Radiation Medicine, Second Military Medical University, Xiangyin Road, Shanghai 200433, PR China.
authors.forename: Cong
authors.initials: C
authors.lastname: Liu
authors.forename: Bailong
authors.initials: B
authors.lastname: Li
authors.forename: Ying
authors.initials: Y
authors.lastname: Cheng
authors.forename: Jing
authors.initials: J
authors.lastname: Lin
authors.forename: Jun
authors.initials: J
authors.lastname: Hao
authors.forename: Shuyu
authors.initials: S
authors.lastname: Zhang
authors.forename: R E J
authors.initials: RE
authors.lastname: Mitchel
authors.forename: Ding
authors.initials: D
authors.lastname: Sun
authors.forename: Jin
authors.initials: J
authors.lastname: Ni
authors.forename: Luqian
authors.initials: L
authors.lastname: Zhao
authors.forename: Fu
authors.initials: F
authors.lastname: Gao
authors.forename: Jianming
authors.initials: J
authors.lastname: Cai
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics / Q000528:radiation effects; D049109:Cell Proliferation / Q000528:radiation effects; D016326:Extracellular Matrix Proteins / Q000235:genetics* / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D008223:Lymphoma / Q000235:genetics* / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D035683:MicroRNAs / Q000502:physiology*; D009381:Neoplasms, Radiation-Induced / Q000235:genetics* / Q000378:metabolism; D013953:Thymus Neoplasms / Q000235:genetics* / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000235:genetics* / Q000378:metabolism; D000099222:betaIG-H3 Protein
chemical_list: D016326:Extracellular Matrix Proteins; D035683:MicroRNAs; D016212:Transforming Growth Factor beta; D000099222:betaIG-H3 Protein; C531950:MIRN21 microRNA, mouse