title: Site-specific proteomic analysis of lipoxidation adducts in cardiac mitochondria reveals chemical diversity of 2-alkenal adduction.
abstract: The modification of proteins by lipid peroxidation products has been linked to numerous diseases and age-related disorders. Here we report on the identification of endogenous protein targets of electrophilic 2-alkenals in cardiac mitochondria. An aldehyde/keto-specific chemical labeling and affinity strategy in combination with LC-MS/MS resulted in 39 unique lipoxidation sites on 27 proteins. Several of the target sites were modified by a variety of 2-alkenal products including acrolein, β-hydroxyacrolein, crotonaldehyde, 4-hydroxy-2-hexenal, 4-hydroxy-2-nonenal and 4-oxo-2-nonenal. Many of the adduction sites are implicated in the catalytic function of key mitochondrial enzymes suggesting potential impact on pathways and overall mitochondrial function.
authors.affiliation: Department of Chemistry, Oregon State University, Corvallis, OR 97331, USA.
authors.forename: Juan D
authors.initials: JD
authors.lastname: Chavez
authors.forename: Jianyong
authors.initials: J
authors.lastname: Wu
authors.forename: William
authors.initials: W
authors.lastname: Bisson
authors.forename: Claudia S
authors.initials: CS
authors.lastname: Maier
mesh_terms: D000447:Aldehydes / Q000737:chemistry / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D002853:Chromatography, Liquid; D015227:Lipid Peroxidation / Q000187:drug effects* / Q000502:physiology; D008297:Male; D008929:Mitochondria, Heart / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism*; D008954:Models, Biological; D008958:Models, Molecular; D010084:Oxidation-Reduction / Q000187:drug effects; D010449:Peptide Mapping / Q000379:methods*; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D040901:Proteomics / Q000379:methods*; D051381:Rats; D011916:Rats, Inbred F344; D013552:Swine; D053719:Tandem Mass Spectrometry
chemical_list: D000447:Aldehydes