title: Reporting from the field: genetically encoded fluorescent reporters uncover signaling dynamics in living biological systems.
abstract: Real-time visualization of a wide range of biochemical processes in living systems is being made possible through the development and application of genetically encoded fluorescent reporters. These versatile biosensors have proven themselves tailor-made to the study of signal transduction, and in this review, we discuss some of the unique insights that they continue to provide regarding the spatial organization and dynamic regulation of intracellular signaling networks. In addition, we explore the more recent push to expand the scope of biological phenomena that can be monitored using these reporters, while also considering the potential to integrate this highly adaptable technology with a number of emerging techniques that may significantly broaden our view of how networks of biochemical processes shape larger biological phenomena.
authors.affiliation: Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
authors.forename: Sohum
authors.initials: S
authors.lastname: Mehta
authors.forename: Jin
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002118:Calcium / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D007425:Intracellular Membranes / Q000737:chemistry; D008958:Models, Molecular; D015398:Signal Transduction / Q000502:physiology*
chemical_list: D005456:Fluorescent Dyes; D049452:Green Fluorescent Proteins; D002118:Calcium