title: [Study on absorption ingredients of Plantaginis semen by in vitro everted intestinal sac method].
abstract: OBJECTIVE
  To establish a method for quick investigating the absorption ingredients of Plantaginis semen and guiding the index selection for its quality control.
  
  
  METHODS
  The absorption of three concentrations of Plantaginis semem was investigated with the in vitro everted intestinal sac (VEIS) model The intestinal sac contents of jejunum and ileum were collected at different time and geniposidic acid was detected by HPLC and LC-MS(n) as the representative marker.
  
  
  RESULTS
  Six ingredients could be detected. At different concentrations of Plantaginis semen, geniposidic acid tested by VEIS showed that there was a good linear correlation between the drug absorption from the medium across the intestinal epithelium into the sac contents in various intestines section. The absorption of the gut sacs from 0 to 90 min manifested a significant time-dependent manner. The Ka of geniposidic acid in the jejunum and ileum increased along with the raised dosage of the Plantaginis Semen (P < 0.05), which indicated a passive absorption manner.
  
  
  CONCLUSIONS
  This method can be used as a tool to investigate the absorption ingredients of Plantaginis Semen. Comparing with the jejunum, the ileum can provide more absorption information faster. The optimal incubation time in intestinal sac was 90 min.
authors.affiliation: Shaanxi University of Chinese Medicine, Shaanxi 712046, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Tan
authors.forename: Jingjing
authors.initials: J
authors.lastname: Zhu
authors.forename: Weihao
authors.initials: W
authors.lastname: Wang
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Wang
authors.forename: Jiucheng
authors.initials: J
authors.lastname: Cui
authors.forename: Jingjing
authors.initials: J
authors.lastname: Kong
authors.forename: Meng
authors.initials: M
authors.lastname: Qi
authors.forename: Li
authors.initials: L
authors.lastname: Yang
mesh_terms: D000818:Animals; D001826:Body Fluids / Q000737:chemistry / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000493:pharmacokinetics*; D007082:Ileum / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D007408:Intestinal Absorption; D007583:Jejunum / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008954:Models, Biological; D010943:Plantago / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004365:Drugs, Chinese Herbal