title: Surface contamination artificially elevates initial sweat mineral concentrations.
abstract: Several sweat mineral element concentrations decline with serial sampling. Possible causes include reduced dermal mineral concentrations or flushing of surface contamination. The purpose of this study was to simultaneously sample mineral concentrations in transdermal fluid (TDF), sweat, and serum during extended exercise-heat stress to determine if these compartments show the same serial changes during repeat sampling. Sixteen heat-acclimated individuals walked on a treadmill (1.56 m/s, 3.0% grade) in a 35°C, 20% relative humidity (RH), 1 m/s wind environment 50 min each hour for 3 h. Mineral concentrations of Ca, Cu, Fe, K, Mg, Na, and Zn were measured each hour from serum, sweat from upper back (sweat pouch) and arm (bag), and TDF from the upper back. Sites were meticulously cleaned to minimize surface contamination. Mineral concentrations were determined by spectrometry. TDF remained stable over time, with exception of a modest increase in TDF [Fe] (15%) and decrease in TDF [Zn] (-18%). Likewise, serum and pouch sweat samples were stable over time. In contrast, the initial arm bag sweat mineral concentrations were greater than those in the sweat pouch, and [Ca], [Cu], [Mg], and [Zn] declined 26-76% from initial to the subsequent samples, becoming similar to sweat pouch. Nominal TDF mineral shifts do not affect sweat mineral concentrations. Arm bag sweat mineral concentrations are initially elevated due to skin surface contaminants that are not removed despite meticulous cleaning (e.g., under fingernails, on arm hair), then decrease with extended sweating and approach those measured from the scapular region.
authors.affiliation: U.S. Army Research Institute of Environmental Medicine, Kansas St., Bldg. 42, Natick, MA 01760-5007, USA. matthew.ely@us.army.mil
authors.forename: Matthew R
authors.initials: MR
authors.lastname: Ely
authors.forename: Robert W
authors.initials: RW
authors.lastname: Kenefick
authors.forename: Samuel N
authors.initials: SN
authors.lastname: Cheuvront
authors.forename: Troy D
authors.initials: TD
authors.lastname: Chinevere
authors.forename: Craig P
authors.initials: CP
authors.lastname: Lacher
authors.forename: Henry C
authors.initials: HC
authors.lastname: Lukaski
authors.forename: Scott J
authors.initials: SJ
authors.lastname: Montain
mesh_terms: D000328:Adult; D015444:Exercise; D005080:Exercise Test; D005260:Female; D018882:Heat Stress Disorders / Q000378:metabolism* / Q000503:physiopathology; D006801:Humans; D008297:Male; D008903:Minerals / Q000097:blood / Q000378:metabolism*; D015203:Reproducibility of Results; D012867:Skin / Q000378:metabolism*; D013048:Specimen Handling / Q000379:methods*; D013057:Spectrum Analysis; D013542:Sweat / Q000378:metabolism*; D013546:Sweating; D013997:Time Factors; D016138:Walking; D055815:Young Adult
chemical_list: D008903:Minerals