title: In vitro antitumor activity of silybin nanosuspension in PC-3 cells.
abstract: The present study aims to evaluate the antitumor activity of silybin nanosuspension on human prostatic carcinoma PC-3 cell line in vitro. Silybin nanosuspension was prepared by the high pressure homogenization (HPH) method. MTT assay, observation of morphological changes and apoptotic body showed that silybin nanosuspension could significantly enhance the in vitro cytotoxicity against PC-3 cells compared to the silybin solution. Flow cytometric (FCM) analysis demonstrated that silybin nanosuspension induced G1 cycle arrest and apoptosis in PC-3 cells. Thereby, the overall results suggest that the silybin nanosuspension represents a potential source of medicine for the treatment of human prostate cancer.
authors.affiliation: College of Pharmacy, Shandong University, 44 Wenhua Xi Road, Jinan, PR China. zhangdianrui2006@163.com
authors.forename: Dandan
authors.initials: D
authors.lastname: Zheng
authors.forename: Yancai
authors.initials: Y
authors.lastname: Wang
authors.forename: Dianrui
authors.initials: D
authors.lastname: Zhang
authors.forename: Zhaoping
authors.initials: Z
authors.lastname: Liu
authors.forename: Cunxian
authors.initials: C
authors.lastname: Duan
authors.forename: Lejiao
authors.initials: L
authors.lastname: Jia
authors.forename: Feihu
authors.initials: F
authors.lastname: Wang
authors.forename: Yue
authors.initials: Y
authors.lastname: Liu
authors.forename: Guangpu
authors.initials: G
authors.lastname: Liu
authors.forename: Leilei
authors.initials: L
authors.lastname: Hao
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D005434:Flow Cytometry; D006801:Humans; D066298:In Vitro Techniques; D008856:Microscopy, Fluorescence; D000077385:Silybin; D012838:Silymarin / Q000494:pharmacology*
chemical_list: D012838:Silymarin; D000077385:Silybin