title: The C-terminus of MIP-T3 protein is required for ubiquitin-proteasome-mediated degradation in human cells.
abstract: The intraflagellar transport (IFT) complex is essential for the formation and functional maintenance of eukaryotic cilia which play a vital role in development and tissue homeostasis. However, the biochemical characteristics and precise functions of IFT proteins remain unknown. Here, we report that MIP-T3, a human microtubule-interacting protein recently identified as a novel conserved component of the IFT complex, is an easily degradable protein in human cell lines. Protein degradation is mediated by the ubiquitin-proteasome system, and the C-terminus is required for ubiquitination and proteasome-mediated degradation of MIP-T3 protein. This study provides the first evidence for regulation of IFT protein stability.
authors.affiliation: Division of Molecular Pharmacology of Infectious Agents, Department of Molecular Microbiology and Immunology, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan.
authors.forename: Chao-Wan
authors.initials: CW
authors.lastname: Guo
authors.forename: Ge
authors.initials: G
authors.lastname: Liu
authors.forename: Sheng
authors.initials: S
authors.lastname: Xiong
authors.forename: Feng
authors.initials: F
authors.lastname: Ge
authors.forename: Takayuki
authors.initials: T
authors.lastname: Fuse
authors.forename: Yi-Fei
authors.initials: YF
authors.lastname: Wang
authors.forename: Kaio
authors.initials: K
authors.lastname: Kitazato
mesh_terms: D015153:Blotting, Western; D045744:Cell Line, Tumor; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D025801:Ubiquitin / Q000378:metabolism*
chemical_list: D008869:Microtubule-Associated Proteins; C412724:TRAF3IP1 protein, human; D025801:Ubiquitin; D049452:Green Fluorescent Proteins; D046988:Proteasome Endopeptidase Complex