title: Adipose injury-associated factors mitigate hypoxia in ischemic tissues through activation of adipose-derived stem/progenitor/stromal cells and induction of angiogenesis.
abstract: Based on the analysis of exudates from injured adipose tissue, we prepared a mixture containing the injury-associated growth factors at the same proportion as the exudates, named adipose injury cocktail (AIC). We hypothesized that AIC induces a series of regenerating and angiogenic processes without actual wounding. The purpose of this study is to elucidate the therapeutic potentials of AIC. AIC preferentially activated adipose-derived stem/progenitor/stromal cells (ASCs) to proliferate, migrate, and form networks compared with vascular endothelial cells, whereas vascular endothelial growth factor did not induce mitogenesis or chemotaxis in human ASCs. Each component growth factor of AIC was differently responsible for the ASC activation. AIC-treated ASCs tended to differentiate into adipocytes or vessel-constituting cells rather than into other cell types. In ischemic adipose tissues of mice, induced by either a surgical intervention or diabetes, AIC administration enhanced proliferation, especially of CD31(-)/CD34(+) ASCs, and mitigated tissue hypoxia by increasing capillary density and reducing fibrogenesis. These results suggest that AIC may have therapeutic potentials for various ischemic/hypoxic conditions by inducing adipose remodeling and neovascularization through activation of ASCs and other cells. Treatment with AIC has many advantages over cell-based therapies regarding morbidity, cost, and physical risks and may be used as an alternative therapy for improving tissue oxygen.
authors.affiliation: Department of Plastic Surgery, University of Tokyo School of Medicine, Tokyo, Japan.
authors.forename: Hitomi
authors.initials: H
authors.lastname: Eto
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Suga
authors.forename: Keita
authors.initials: K
authors.lastname: Inoue
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Aoi
authors.forename: Harunosuke
authors.initials: H
authors.lastname: Kato
authors.forename: Jun
authors.initials: J
authors.lastname: Araki
authors.forename: Kentaro
authors.initials: K
authors.lastname: Doi
authors.forename: Takuya
authors.initials: T
authors.lastname: Higashino
authors.forename: Kotaro
authors.initials: K
authors.lastname: Yoshimura
mesh_terms: D000273:Adipose Tissue / Q000293:injuries / Q000378:metabolism*; D000328:Adult; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002469:Cell Separation; D005434:Flow Cytometry; D006801:Humans; D000860:Hypoxia / Q000378:metabolism / Q000517:prevention & control*; D007150:Immunohistochemistry; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism* / Q000494:pharmacology; D007511:Ischemia / Q000378:metabolism / Q000517:prevention & control*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D051379:Mice; D018919:Neovascularization, Physiologic / Q000187:drug effects / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017154:Stromal Cells / Q000166:cytology / Q000187:drug effects
chemical_list: D036341:Intercellular Signaling Peptides and Proteins