title: High expression of cytoskeletal protein drebrin in TEL/AML1pos B-cell precursor acute lymphoblastic leukemia identified by a novel monoclonal antibody.
abstract: The expression of drebrin, a cytoskeletal protein newly estimated by expression profiling to correlate with the genotype and prognosis of B-cell precursor acute lymphoblastic leukemia (BCP-ALL), was examined by independent methods. After demonstrating its higher expression in TEL/AML1(pos) BCP-ALL by quantitative reverse transcriptase polymerase chain reaction, we developed an anti-drebrin monoclonal antibody (mAb). In a cohort of 86 children with BCP-ALL, we found increased expression of drebrin in TEL/AML1(pos) ALL. In conclusion, relationship of drebrin expression and prognosis or genotype can now be assessed using flow cytometry.
authors.affiliation: Childhood Leukemia Investigation Prague (CLIP), Prague, Czech Republic.
authors.forename: Martina
authors.initials: M
authors.lastname: Vaskova
authors.forename: Martin
authors.initials: M
authors.lastname: Kovac
authors.forename: Petra
authors.initials: P
authors.lastname: Volna
authors.forename: Pavla
authors.initials: P
authors.lastname: Angelisova
authors.forename: Ester
authors.initials: E
authors.lastname: Mejstrikova
authors.forename: Jan
authors.initials: J
authors.lastname: Zuna
authors.forename: Tomas
authors.initials: T
authors.lastname: Brdicka
authors.forename: Ondrej
authors.initials: O
authors.lastname: Hrusak
mesh_terms: D000208:Acute Disease; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D050676:Core Binding Factor Alpha 2 Subunit; D005434:Flow Cytometry; D006801:Humans; D051379:Mice; D009479:Neuropeptides / Q000276:immunology* / Q000378:metabolism*; D015514:Oncogene Proteins, Fusion; D015452:Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000097764:ETS Translocation Variant 6 Protein
chemical_list: D000911:Antibodies, Monoclonal; D050676:Core Binding Factor Alpha 2 Subunit; D009479:Neuropeptides; D015514:Oncogene Proteins, Fusion; D012333:RNA, Messenger; C102421:TEL-AML1 fusion protein; D000097764:ETS Translocation Variant 6 Protein; C053386:drebrins