title: Development of a genetic system for the archaeal virus Sulfolobus turreted icosahedral virus (STIV).
abstract: Our understanding of archaeal viruses has been limited by the lack of genetic systems for examining viral function. We describe the construction of an infectious clone for the archaeal virus Sulfolobus turreted icosahedral virus (STIV). STIV was isolated from a high temperature (82°C) acidic (pH 2.2) hot spring in Yellowstone National Park and replicates in the archaeal model organism Sulfolobus solfataricus (Rice et al., 2004). While STIV is one of most studied archaeal viruses, little is known about its replication cycle. The development of an STIV infectious clone allows for directed gene disruptions and detailed genetic analysis of the virus. The utility of the STIV infectious clone was demonstrated by gene disruption of STIV open reading frame (ORF) B116 which resulted in crippled virus replication, while disruption of ORFs A197, C381 and B345 was lethal for virus replication.
authors.affiliation: Thermal Biology Institute, Montana State University, Bozeman, MT, USA.
authors.forename: Jennifer Fulton
authors.initials: JF
authors.lastname: Wirth
authors.forename: Jamie C
authors.initials: JC
authors.lastname: Snyder
authors.forename: Rebecca A
authors.initials: RA
authors.lastname: Hochstein
authors.forename: Alice C
authors.initials: AC
authors.lastname: Ortmann
authors.forename: Deborah A
authors.initials: DA
authors.lastname: Willits
authors.forename: Trevor
authors.initials: T
authors.lastname: Douglas
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Young
mesh_terms: D023641:Archaeal Viruses / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology / Q000648:ultrastructure; D001483:Base Sequence; D015153:Blotting, Western; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000821:virology; D009154:Mutation; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D016944:Sulfolobus / Q000235:genetics / Q000302:isolation & purification / Q000648:ultrastructure / Q000821:virology*; D014481:United States; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics*; D014779:Virus Replication
chemical_list: D014764:Viral Proteins