title: Cross-species transmission of gibbon and orangutan hepatitis B virus to uPA/SCID mice with human hepatocytes.
abstract: To investigate the potential of cross-species transmission of non-human primate HBV to humans, severe combined immunodeficiency mice transgenic for urokinase-type plasminogen activator, in which the mouse liver has been engrafted with human hepatocytes, were inoculated with non-human primate HBV. HBV-DNA positive serum samples from a gibbon or orangutan were inoculated into 6 chimeric mice. HBV-DNA, hepatitis B surface antigen (HBsAg), and HB core-related antigen in sera and HBV cccDNA in liver were detectable in 2 of 3 mice each from the gibbon and orangutan. Likewise, applying immunofluorescence HBV core protein was only found in human hepatocytes expressing human albumin. The HBV sequences from mouse sera were identical to those from orangutan and gibbon sera determined prior to inoculation. In conclusion, human hepatocytes have been infected with gibbon/orangutan HBV.
authors.affiliation: Center of Excellence in Clinical Virology, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Pattaratida
authors.initials: P
authors.lastname: Sa-Nguanmoo
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Parntep
authors.initials: P
authors.lastname: Ratanakorn
authors.forename: Masaya
authors.initials: M
authors.lastname: Sugiyama
authors.forename: Shuko
authors.initials: S
authors.lastname: Murakami
authors.forename: Sunchai
authors.initials: S
authors.lastname: Payungporn
authors.forename: Angkana
authors.initials: A
authors.lastname: Sommanustweechai
authors.forename: Masashi
authors.initials: M
authors.lastname: Mizokami
authors.forename: Yong
authors.initials: Y
authors.lastname: Poovorawan
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004195:Disease Models, Animal; D006509:Hepatitis B / Q000635:transmission / Q000662:veterinary*; D006515:Hepatitis B virus / Q000302:isolation & purification*; D022781:Hepatocytes / Q000821:virology*; D006801:Humans; D006922:Hylobates; D051379:Mice; D016513:Mice, SCID; D057079:Pongo; D018419:Primate Diseases / Q000635:transmission* / Q000821:virology*; D015047:Zoonoses / Q000635:transmission / Q000821:virology