title: Repair of rat cranial bone defects with nHAC/PLLA and BMP-2-related peptide or rhBMP-2.
abstract: An ideal artificial substitute has good biocompatibility properties and is able to provide for rapid bone formation. Bone morphogenetic protein-2 (BMP-2) is considered as one of the most important growth factors for bone regeneration. In this study, a synthetic BMP-2-related peptide (designated P24) corresponding to residues of the knuckle epitope of BMP-2 was introduced into a bioactive scaffold based on nano-hydroxyapatite/collagen/poly(L-lactic acid) (nHAC/PLLA); its in vitro release kinetics was then measured. A 5 mm diameter cranial bone defect was created in the calvariae of 30 rats and randomly implanted with three groups of biomaterials: Group A (nHAC/PLLA alone); Group B (P24/nHAC/PLLA composite); and Group C (recombinant human BMP-2 (rhBMP-2)/nHAC/PLLA composite). The P24/nHAC/PLLA implants significantly stimulated bone growth similarly to the rhBMP-2/nHAC/PLLA implants based on the radiographic and three-dimensional CT evaluation and histological examination, thereby confirming the enhanced bone healing rate of these compounds compared with the stand-alone nHAC/PLLA scaffold material. The osteoinductive ability of 3 mg P24 was similar to that of 1 µg rhBMP-2. P24/nHAC/PLLA is a promising scaffold biomaterial for bone tissue regeneration.
authors.affiliation: Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, People's Republic of China.
authors.forename: Jingfeng
authors.initials: J
authors.lastname: Li
authors.forename: Jijun
authors.initials: J
authors.lastname: Hong
authors.forename: Qixin
authors.initials: Q
authors.lastname: Zheng
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Guo
authors.forename: Shenghui
authors.initials: S
authors.lastname: Lan
authors.forename: Fuzhai
authors.initials: F
authors.lastname: Cui
authors.forename: Haitao
authors.initials: H
authors.lastname: Pan
authors.forename: Zhenwei
authors.initials: Z
authors.lastname: Zou
authors.forename: Chao
authors.initials: C
authors.lastname: Chen
mesh_terms: D000818:Animals; D001847:Bone Diseases / Q000000981:diagnostic imaging / Q000188:drug therapy*; D055396:Bone Morphogenetic Protein 2 / Q000494:pharmacology*; D001861:Bone Regeneration / Q000187:drug effects* / Q000502:physiology; D003094:Collagen / Q000494:pharmacology*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D017886:Durapatite / Q000494:pharmacology*; D006801:Humans; D019344:Lactic Acid / Q000494:pharmacology*; D008297:Male; D010455:Peptides / Q000494:pharmacology; D011091:Polyesters; D011108:Polymers / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000494:pharmacology; D012886:Skull / Q000000981:diagnostic imaging / Q000187:drug effects* / Q000502:physiology; D054457:Tissue Scaffolds; D014057:Tomography, X-Ray Computed; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
chemical_list: D055396:Bone Morphogenetic Protein 2; D010455:Peptides; D011091:Polyesters; D011108:Polymers; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C509317:nano-hydroxyapatite-collagen; C499469:recombinant human bone morphogenetic protein-2; D019344:Lactic Acid; C033616:poly(lactide); D003094:Collagen; D017886:Durapatite