title: Novel biphenyl-oxidizing bacteria and dioxygenase genes from a korean tidal mudflat.
abstract: Gene-targeted FLX titanium pyrosequencing integrated with stable isotope probing (SIP) using [(13)C]biphenyl substrate revealed that tidal mudflat sediments harbor novel aromatic ring hydroxylating dioxygenases (ARHD). More than 80% of the detected ARHD genes comprise four clades (0.5 distance) with 49 to 70% amino acid identity to sequences in public databases. The 16S rRNA sequences enriched in the (13)C fraction were from the Betaproteobacteria, bacilli (primarily Paenibacillus-like), and unclassified phyla.
authors.affiliation: School of Civil and Environmental Engineering, Yonsei University, Seoul 120-749, Republic of Korea.
authors.forename: Tae Kwon
authors.initials: TK
authors.lastname: Lee
authors.forename: Jaejin
authors.initials: J
authors.lastname: Lee
authors.forename: Woo Jun
authors.initials: WJ
authors.lastname: Sul
authors.forename: Shoko
authors.initials: S
authors.lastname: Iwai
authors.forename: Benli
authors.initials: B
authors.lastname: Chai
authors.forename: James M
authors.initials: JM
authors.lastname: Tiedje
authors.forename: Joonhong
authors.initials: J
authors.lastname: Park
mesh_terms: D001419:Bacteria / Q000145:classification / Q000201:enzymology* / Q000302:isolation & purification / Q000378:metabolism*; D001713:Biphenyl Compounds / Q000378:metabolism*; D002247:Carbon Isotopes / Q000378:metabolism; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D049308:Dioxygenases / Q000235:genetics* / Q000378:metabolism; D004783:Environmental Microbiology; D007553:Isotope Labeling; D010084:Oxidation-Reduction; D010802:Phylogeny; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D001713:Biphenyl Compounds; D002247:Carbon Isotopes; D004269:DNA, Bacterial; D049308:Dioxygenases; C010574:biphenyl