title: Genomics for key players in the N cycle from guinea pigs to the next frontier.
abstract: While sequencing methods were available in the late 1970s, it was not until the human genome project and a significant influx of funds for such research that this technology became high throughput. The fields of microbiology and microbial ecology, among many others, have been tremendously impacted over the years, to such an extent that the determination of complete microbial genome sequences is now commonplace. Given the lower costs of next-generation sequencing platforms, even small laboratories from around the world will be able to generate millions of base pairs of data, equivalent to entire genomes worth of sequence information. With this prospect just around the corner, it is timely to provide an overview of the genomics process: from sample preparation to some of the analytical methods used to gain functional knowledge from sequence information.
authors.affiliation: Genome Biology Group, Los Alamos National Laboratory, Los Alamos, New Mexico, USA.
authors.forename: Patrick S G
authors.initials: PS
authors.lastname: Chain
authors.forename: Gary
authors.initials: G
authors.lastname: Xie
authors.forename: Shawn R
authors.initials: SR
authors.lastname: Starkenburg
authors.forename: Matthew B
authors.initials: MB
authors.lastname: Scholz
authors.forename: Nicholas
authors.initials: N
authors.lastname: Beckloff
authors.forename: Chien-Chi
authors.initials: CC
authors.lastname: Lo
authors.forename: Karen W
authors.initials: KW
authors.lastname: Davenport
authors.forename: Krista G
authors.initials: KG
authors.lastname: Reitenga
authors.forename: Hajnalka E
authors.initials: HE
authors.lastname: Daligault
authors.forename: J Chris
authors.initials: JC
authors.lastname: Detter
authors.forename: Tracey A K
authors.initials: TA
authors.lastname: Freitas
authors.forename: Cheryl D
authors.initials: CD
authors.lastname: Gleasner
authors.forename: Lance D
authors.initials: LD
authors.lastname: Green
authors.forename: Cliff S
authors.initials: CS
authors.lastname: Han
authors.forename: Kim K
authors.initials: KK
authors.lastname: McMurry
authors.forename: Linda J
authors.initials: LJ
authors.lastname: Meincke
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Shen
authors.forename: Ahmet
authors.initials: A
authors.lastname: Zeytun
mesh_terms: D001419:Bacteria / Q000235:genetics* / Q000378:metabolism; D016680:Genome, Bacterial; D023281:Genomics / Q000379:methods*; D058977:Molecular Sequence Annotation / Q000379:methods*; D058465:Nitrification / Q000235:genetics*; D058458:Nitrogen Cycle / Q000235:genetics*; D043370:Nitrosomonas europaea / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA / Q000379:methods*