title: Quantum dot-aptamer nanoprobes for recognizing and labeling influenza A virus particles.
abstract: The fluorescence labeling of viruses is a useful technology for virus detection and imaging. By combining the excellent fluorescence properties of quantum dots (QDs) with the high affinity and specificity of aptamers, we constructed a QD-aptamer probe. The aptamer A22, against the hemagglutinin of influenza A virus, was linked to QDs, producing the QD-A22 probe. Fluorescence imaging and transmission electron microscopy showed that the QD-A22 probe could specifically recognize and label influenza A virus particles. This QD labeling technique provides a new strategy for labeling virus particles for virus detection and imaging.
authors.affiliation: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China.
authors.forename: Zong-Qiang
authors.initials: ZQ
authors.lastname: Cui
authors.forename: Qian
authors.initials: Q
authors.lastname: Ren
authors.forename: Hong-Ping
authors.initials: HP
authors.lastname: Wei
authors.forename: Ze
authors.initials: Z
authors.lastname: Chen
authors.forename: Jiao-Yu
authors.initials: JY
authors.lastname: Deng
authors.forename: Zhi-Ping
authors.initials: ZP
authors.lastname: Zhang
authors.forename: Xian-En
authors.initials: XE
authors.lastname: Zhang
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D009980:Influenza A virus / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D045663:Quantum Dots
chemical_list: D052157:Aptamers, Nucleotide