title: Single-shot full-field reflection phase microscopy.
abstract: We present a full-field reflection phase microscope that combines low-coherence interferometry and off-axis digital holographic microscopy (DHM). The reflection-based DHM provides highly sensitive and a single-shot imaging of cellular dynamics while the use of low coherence source provides a depth-selective measurement. The setup uniquely uses a diffraction grating in the reference arm to generate an interference image of uniform contrast over the entire field-of-view albeit low-coherence light source. We have measured the path-length sensitivity of our instrument to be approximately 21 picometers/Hz that makes it suitable for nanometer-scale full-field measurement of membrane dynamics in live cells.
authors.affiliation: G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA. zyaqoob@mit.edu
authors.forename: Zahid
authors.initials: Z
authors.lastname: Yaqoob
authors.forename: Toyohiko
authors.initials: T
authors.lastname: Yamauchi
authors.forename: Wonshik
authors.initials: W
authors.lastname: Choi
authors.forename: Dan
authors.initials: D
authors.lastname: Fu
authors.forename: Ramachandra R
authors.initials: RR
authors.lastname: Dasari
authors.forename: Michael S
authors.initials: MS
authors.lastname: Feld
mesh_terms: D000465:Algorithms; D002462:Cell Membrane / Q000378:metabolism; D004867:Equipment Design; D006367:HeLa Cells; D006801:Humans; D007368:Interferometry / Q000295:instrumentation / Q000379:methods; D008027:Light; D008858:Microscopy, Phase-Contrast / Q000295:instrumentation* / Q000379:methods; D015233:Models, Statistical; D009038:Motion; D055095:Optics and Photonics; D012815:Signal Processing, Computer-Assisted