title: Identification and cloning of immunogenic Aliivibrio salmonicida Pal-like protein present in profiled outer membrane and secreted subproteome.
abstract: Aliivibrio salmonicida is the aetiological agent of cold water vibriosis affecting farmed fish species, a disease that today is fully controlled by vaccination. However, the molecular mechanisms behind the successful vaccine are largely unknown. In order to gain insight into the possible mechanisms of A. salmonicida vaccines, we report here the profiles of both the outer membrane and secreted subproteomes of A. salmonicida LFI315. The 2 subproteomes were resolved by 2-dimensional electrophoresis that identified a total of 82 protein entries. Monoclonal antibodies specific to an unidentified protein antigen were utilized in the immunoproteomic analysis of both outer membrane proteins and extracellular proteins. The immunogenic protein was located in both subproteomes and identified as a 20 kDa peptidoglycan-associated lipoprotein (Pal). The identity of the antigen was verified by heterologous expression of the cloned A. salmonicida pal gene (VSAL_I1899). It is likely that the immunogenic Pal-like protein is among the constituents that act as a protective antigen in the successful vaccine used today. In view of this, it may be considered a potentially useful component in future vaccine development and pathogenicity studies.
authors.affiliation: Department of Molecular Biotechnology, Institute of Medical Biology, Faculty of Medicine, University of Tromso, 9037 Tromse, Norway.
authors.forename: Christian
authors.initials: C
authors.lastname: Karlsen
authors.forename: Sigrun
authors.initials: S
authors.lastname: Espelid
authors.forename: Nils-Peder
authors.initials: NP
authors.lastname: Willassen
authors.forename: Steinar M
authors.initials: SM
authors.lastname: Paulsen
mesh_terms: D044165:Aliivibrio salmonicida / Q000235:genetics* / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D020543:Proteome
chemical_list: D001425:Bacterial Outer Membrane Proteins; D020543:Proteome