title: Over-expression of RAD51 or RAD54 but not RAD51/4 enhances extra-chromosomal homologous recombination in the human sarcoma (HT-1080) cell line.
abstract: RAD51 and RAD54, members of the RAD52 epistasis group, play key roles in homologous recombination (HR). The efficiency of homologous recombination (HR) can be increased by over-expression of either of them. A vector that allows co-expression of RAD51 and RAD54 was constructed to investigate interactions between the two proteins during extra-chromosomal HR. The efficiency of extra-chromosomal HR evaluated by GFP extra-chromosomal HR was enhanced (110-245%) in different transfected Human sarcoma (HT-1080) cell colonies. We observed that RAD51 clearly promotes extra-chromosomal HR; however, the actions of RAD54 in extra-chromosomal HR were weak. Our data suggest that RAD51 may function as a universal factor during HR, whereas RAD54 mainly functions in other types of HR (gene targeting or intra-chromosomal HR), which involves interaction with chromosomal DNA.
authors.affiliation: State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing, People's Republic of China. victory811111@126.com
authors.forename: Shengli
authors.initials: S
authors.lastname: Yu
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Song
authors.forename: Junjie
authors.initials: J
authors.lastname: Luo
authors.forename: Yunping
authors.initials: Y
authors.lastname: Dai
authors.forename: Ning
authors.initials: N
authors.lastname: Li
mesh_terms: D015153:Blotting, Western; D045744:Cell Line, Tumor; D002877:Chromosomes, Human / Q000235:genetics*; D004265:DNA Helicases; D004268:DNA-Binding Proteins; D005434:Flow Cytometry; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D009687:Nuclear Proteins / Q000235:genetics*; D051135:Rad51 Recombinase / Q000235:genetics*; D011995:Recombination, Genetic; D012509:Sarcoma / Q000235:genetics*; D019076:Transgenes / Q000235:genetics
chemical_list: D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; C495153:RAD51 protein, human; D051135:Rad51 Recombinase; D004265:DNA Helicases; C103532:RAD54L protein, human