title: [Effect of brucine on secretion function and proliferation capability of T lymphocytes in patients with aplastic anemia].
abstract: The aim of this study was to investigate the effect of brucine on secretion of TNF-α, IFN-γ, IL-4 and proliferation of T lymphocytes in patients with aplastic anemia (AA), and to explore its mechanism. Peripheral blood T lymphocytes from 10 patients with AA and 10 healthy volunteers were isolated, purified and cultured. T lymphocytes from the patients were divided into 0, 100, 200 and 400 µg/ml brucine-treated groups. T lymphocytes from healthy volunteers were used as control group. After being cultured for 72 hours, the levels of TNF-α, IFN-γ, IL-4 in the supernatant of cultured T lymphocytes from AA patients were detected by ELISA, and the proliferation of T lymphocytes from AA patients was detected by MTT. The results showed that compared with the normal control group, the levels of TNF-α and IFN-γ in the culture supernatant significantly increased, and IL-4 was significantly decreased. The levels of TNF-α and IFN-γ in the culture supernatant of brucine treated groups were lower, and were dependent on the concentration of brucine. However, the levels of IL-4 were found to be not obviously changed. The inhibition rate of T lymphocytes in 100, 200 and 400µg/ml brucine-treated groups were (13.61 ± 4.31)%, (14.28 ± 4.31)% and (15.12 ± 4.56)% respectively, among which the differences were not statistically significant. It is concluded that the brucine can reduce the levels of TNF-α and IFN-γ through inhibiting the proliferation of T lymphocytes in AA patients, which provides experimental basis for therapy of AA patients.
authors.affiliation: Shanxi Medical University First Clinical Hospital, Taiyuan 030001, Shanxi Province, China.
authors.forename: Chun-Yan
authors.initials: CY
authors.lastname: Zhai
authors.forename: Wu
authors.initials: W
authors.lastname: Wei
authors.forename: Ai-Fang
authors.initials: AF
authors.lastname: Ji
authors.forename: Xu-Liang
authors.initials: XL
authors.lastname: Shen
authors.forename: Guo-Xiang
authors.initials: GX
authors.lastname: Zhang
authors.forename: Jian-Bin
authors.initials: JB
authors.lastname: Yang
authors.forename: Hai-Li
authors.initials: HL
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Liang
authors.forename: Ming-Xia
authors.initials: MX
authors.lastname: Wei
mesh_terms: D000293:Adolescent; D000328:Adult; D000741:Anemia, Aplastic / Q000276:immunology / Q000378:metabolism*; D016022:Case-Control Studies; D049109:Cell Proliferation; D002478:Cells, Cultured; D002648:Child; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D013331:Strychnine / Q000031:analogs & derivatives* / Q000494:pharmacology; D013601:T-Lymphocytes / Q000166:cytology / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D055815:Young Adult
chemical_list: C508598:IL4 protein, human; D014409:Tumor Necrosis Factor-alpha; D015847:Interleukin-4; C083806:brucine; D007371:Interferon-gamma; D013331:Strychnine