title: A rhodamine-based "turn-on" fluorescent chemodosimeter for Cu2+ and its application in living cell imaging.
abstract: A new fluorescent probe 1, N-(Rhodamine-6G)lactam-hydrazinecarbothioamide, was synthesized as a fluorescent and colorimetric chemodosimeter in aqueous solution for Cu(2+). Following Cu(2+)-promoted ring opening, redox and hydrolysis reactions, comparable amplifications of absorption and fluorescence signals were observed upon addition of Cu(2+); this suggests that chemodosimeter 1 effectively avoided the fluorescence quenching caused by the paramagnetic nature of Cu(2+). Importantly, 1 can selectively recognize Cu(2+) in aqueous media in the presence of other trace metal ions in organisms, abundant cellular cations and the prevalent toxic metal ions in the environment with high sensitivity (detection limit <3 ppb) and a rapid response time (<2 min). In addition, the biological imaging study has demonstrated that 1 can detect Cu(2+) in the living cells.
authors.affiliation: Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province, State Key Laboratory of Applied Organic Chemistry and College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, PR China.
authors.forename: Liang
authors.initials: L
authors.lastname: Huang
authors.forename: Feng-ping
authors.initials: FP
authors.lastname: Hou
authors.forename: Pinxian
authors.initials: P
authors.lastname: Xi
authors.forename: Decheng
authors.initials: D
authors.lastname: Bai
authors.forename: Min
authors.initials: M
authors.lastname: Xu
authors.forename: Zhengpeng
authors.initials: Z
authors.lastname: Li
authors.forename: Gouqiang
authors.initials: G
authors.lastname: Xie
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Shi
authors.forename: Hongyan
authors.initials: H
authors.lastname: Liu
authors.forename: Zhengzhi
authors.initials: Z
authors.lastname: Zeng
mesh_terms: D045744:Cell Line, Tumor; D003300:Copper / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D057230:Limit of Detection; D018613:Microscopy, Confocal; D012235:Rhodamines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: C560854:1,N-(rhodamine-6G)lactam-hydrazinecarbothioamide; D005456:Fluorescent Dyes; D012235:Rhodamines; C026188:rhodamine 6G; D003300:Copper