title: Kaurenoic acid from pulp of Annona cherimolia in regard to Annonaceae-induced Parkinsonism.
abstract: Guadeloupean Parkinsonism has been linked epidemiologically to the consumption of Annonaceae fruits. These were proposed to be etiological agents for sporadic atypical Parkinsonism worldwide, because of their content of neurotoxins such as isoquinolinic alkaloids and Annonaceous acetogenins. The pulp of Annona cherimolia Mill. from Spain was screened for these toxic molecules using Matrix-Assisted Laser Desorption Ionisation - Time of Flight mass spectrometry (MALDI-TOF MS) and it was found not to be a source of exposure. However, kaurenoic acid, a diterpene considered to be cytotoxic, was detected in high amounts (66 mg/fresh fruit). Treatment of rat embryonic striatal primary cultures, up to a high concentration (50 µM), did not cause neuronal death nor astrogliosis, suggesting that this molecule is not at risk of implication in human neurodegenerative diseases.
authors.affiliation: Laboratoire de Pharmacognosie, CNRS UMR 8076 BioCIS, Faculté de Pharmacie, Université Paris-Sud 11, 92296, Châtenay-Malabry, France.
authors.forename: R
authors.initials: R
authors.lastname: Guillopé
authors.forename: M
authors.initials: M
authors.lastname: Escobar-Khondiker
authors.forename: V
authors.initials: V
authors.lastname: Guérineau
authors.forename: O
authors.initials: O
authors.lastname: Laprévote
authors.forename: G U
authors.initials: GU
authors.lastname: Höglinger
authors.forename: P
authors.initials: P
authors.lastname: Champy
mesh_terms: D000818:Animals; D031041:Annona / Q000009:adverse effects* / Q000737:chemistry; D001253:Astrocytes / Q000187:drug effects; D002478:Cells, Cultured; D004224:Diterpenes / Q000032:analysis* / Q000302:isolation & purification; D005638:Fruit / Q000737:chemistry; D009498:Neurotoxins / Q000032:analysis / Q000302:isolation & purification; D020734:Parkinsonian Disorders / Q000139:chemically induced*; D051381:Rats; D017208:Rats, Wistar; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D004224:Diterpenes; D009498:Neurotoxins; C051436:kaurenoic acid