title: Kinetic resolution of 3-fluoroalanine using a fusion protein of D-amino acid oxidase with Vitroscilla hemoglobin.
abstract: In this study, a fusion protein (VHb-DAAO) of D-amino acid oxidase (DAAO) with Vitreoscilla hemoglobin (VHb) was functionally expressed in Escherichia coli and purified. The k(cat) value VHb-DAAO (47.1 s⁻¹) towards rac-3-flouroalanine was about 2-fold higher than that of DAAO (21.9 s⁻¹). rac-3-Flouroalanine (500 mM) was kinetically resolved into (R)-3-fluoroalanine with high enatiomeric excess (>99%) by VHb-DAAO with about 52% conversion.
authors.affiliation: School of Biotechnology, Yeungnam University, Japan.
authors.forename: Young-Man
authors.initials: YM
authors.lastname: Seo
authors.forename: Yong-Ho
authors.initials: YH
authors.lastname: Khang
authors.forename: Hyungdon
authors.initials: H
authors.lastname: Yun
mesh_terms: D000409:Alanine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics*; D003605:D-Amino-Acid Oxidase / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D005560:Formate Dehydrogenases / Q000378:metabolism; D007700:Kinetics; D009243:NAD / Q000378:metabolism; D010084:Oxidation-Reduction; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012248:Rhodotorula / Q000201:enzymology; D013237:Stereoisomerism; D054793:Truncated Hemoglobins / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D011993:Recombinant Fusion Proteins; D054793:Truncated Hemoglobins; D009243:NAD; C082613:hemoglobin protein, Vitreoscilla; C007742:3-fluoroalanine; D005560:Formate Dehydrogenases; D003605:D-Amino-Acid Oxidase; D000409:Alanine