title: In vitro investigation of the cellular toxicity of boron nitride nanotubes.
abstract: Nanotubes present one of the most promising opportunities in nanotechnology with a plethora of applications in nanoelectronics, mechanical engineering, as well as in biomedical technology. Due to their structure and some physical properties, boron nitride (BN) nanotubes (BNNTs) possess several advantages over carbon nanotubes (CNTs), and they are now commercially produced and used on a large scale. The human and environmental exposure to BN nanomaterials is expected to increase in the near future, and their biological responses need to be examined. Using complementary assays, we have extensively investigated the effects of BNNTs on the viability and metabolic status of different cell types: on the one hand, the effects on cells present in the lung alveoli, and on the other hand, on human embryonic kidney (HEK) cells. Our results indicate that BNNTs are cytotoxic for all cell types studied and, in most cases, are more cytotoxic than CNTs in their pristine (p-CNT) and functionalized (f-CNT) form. However, the level of toxicity and the prominent morphological alterations in the cell populations withstanding BNNT exposure are cell-type-dependent. For instance, BNNTs induced extensive multinucleated giant cell formation in macrophages and increased levels of eosinophilia in fibroblasts. Finally, our results point the toxicity of tubular nanomaterials to be strongly correlated with the cellular accumulation enhanced for straight nanotubes.
authors.affiliation: Laboratory of Physics of Complex Matter (LPMC), Ecole Polytechnique Fédérale de Lausanne, 1015 Lausanne, Switzerland.
authors.forename: Lenke
authors.initials: L
authors.lastname: Horváth
authors.forename: Arnaud
authors.initials: A
authors.lastname: Magrez
authors.forename: Dmitri
authors.initials: D
authors.lastname: Golberg
authors.forename: Chunyi
authors.initials: C
authors.lastname: Zhi
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Bando
authors.forename: Rita
authors.initials: R
authors.lastname: Smajda
authors.forename: Endre
authors.initials: E
authors.lastname: Horváth
authors.forename: László
authors.initials: L
authors.lastname: Forró
authors.forename: Beat
authors.initials: B
authors.lastname: Schwaller
mesh_terms: D001896:Boron Compounds / Q000633:toxicity*; D002470:Cell Survival / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D057809:HEK293 Cells; D006801:Humans; D043942:Nanotubes / Q000633:toxicity*
chemical_list: D001896:Boron Compounds; C017282:boron nitride