title: Comparison of the dose-response relationship of radiation-induced apoptosis in the hippocampal dentate gyrus and intestinal crypt of adult mice.
abstract: The present study compared the dose-response curves for the frequency of apoptosis in mouse hippocampal dentate gyrus (DG) and intestinal crypt using whole-body gamma irradiation. The incidence of gamma-ray-induced apoptosis was measured using the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end-labelling (TUNEL) method. TUNEL-positive apoptotic nuclei in the DG and intestinal crypt were increased in a dose-dependent pattern (0-2 Gy). The dose-response curves were linear-quadratic, with a significant relationship between the appearance of apoptosis and irradiation dose. The slopes of the dose-response curves in the DG were much steeper (~5-6-fold) than those in the intestinal crypt within the range of 0-1 Gy exposure. Hippocampal DG might be a more effective and sensitive evaluation structure than the intestinal crypt to estimate the degree of radiation exposure in damaged organs of adult mice exposed to low irradiation dose.
authors.affiliation: Research Center, Dongnam Institute of Radiological and Medical Science, Busan 619-753, South Korea.
authors.forename: J S
authors.initials: JS
authors.lastname: Kim
authors.forename: M
authors.initials: M
authors.lastname: Yang
authors.forename: J
authors.initials: J
authors.lastname: Kim
authors.forename: D
authors.initials: D
authors.lastname: Lee
authors.forename: J C
authors.initials: JC
authors.lastname: Kim
authors.forename: T
authors.initials: T
authors.lastname: Shin
authors.forename: S H
authors.initials: SH
authors.lastname: Kim
authors.forename: C
authors.initials: C
authors.lastname: Moon
mesh_terms: D000818:Animals; D017209:Apoptosis; D002467:Cell Nucleus / Q000378:metabolism; D003037:Cobalt Radioisotopes / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D006624:Hippocampus / Q000473:pathology* / Q000528:radiation effects*; D020287:In Situ Nick-End Labeling; D007422:Intestines / Q000473:pathology* / Q000528:radiation effects*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008856:Microscopy, Fluorescence / Q000379:methods; D020534:Parahippocampal Gyrus / Q000473:pathology* / Q000528:radiation effects*; D011829:Radiation Dosage; D011874:Radiometry / Q000379:methods*
chemical_list: D003037:Cobalt Radioisotopes