title: Regulation of Substantia Nigra Pars Reticulata GABAergic Neuron Activity by H₂O₂ via Flufenamic Acid-Sensitive Channels and KATPChannels.
abstract: Substantia nigra pars reticulata (SNr) GABAergic neurons are key output neurons of the basal ganglia. Given the role of these neurons in motor control, it is important to understand factors that regulate their firing rate and pattern. One potential regulator is hydrogen peroxide (H₂O₂), a reactive oxygen species that is increasingly recognized as a neuromodulator. We used whole-cell current clamp recordings of SNr GABAergic neurons in guinea-pig midbrain slices to determine how H₂O₂ affects the activity of these neurons and to explore the classes of ion channels underlying those effects. Elevation of H₂O₂ levels caused an increase in the spontaneous firing rate of SNr GABAergic neurons, whether by application of exogenous H₂O₂ or amplification of endogenous H₂O₂ through inhibition of glutathione peroxidase with mercaptosuccinate. This effect was reversed by flufenamic acid (FFA), implicating transient receptor potential (TRP) channels. Conversely, depletion of endogenous H₂O₂ by catalase, a peroxidase enzyme, decreased spontaneous firing rate and firing precision of SNr neurons, demonstrating tonic control of firing rate by H₂O₂. Elevation of H₂O₂ in the presence of FFA revealed an inhibition of tonic firing that was prevented by blockade of ATP-sensitive K(+) (K(ATP)) channels with glibenclamide. In contrast to guinea-pig SNr neurons, the dominant effect of H₂O₂ elevation in mouse SNr GABAergic neurons was hyperpolarization, indicating a species difference in H₂O₂-dependent regulation. Thus, H₂O₂ is an endogenous modulator of SNr GABAergic neurons, acting primarily through presumed TRP channels in guinea-pig SNr, with additional modulation via K(ATP) channels to regulate SNr output.
authors.affiliation: Department of Neurosurgery, New York University School of Medicine New York, NY, USA.
authors.forename: Christian R
authors.initials: CR
authors.lastname: Lee
authors.forename: Paul
authors.initials: P
authors.lastname: Witkovsky
authors.forename: Margaret E
authors.initials: ME
authors.lastname: Rice