title: Development of a pyrosequencing assay for rapid assessment of quinolone resistance in Acinetobacter baumannii isolates.
abstract: Rapid and reliable assessment of Acinetobacter baumannii resistance to quinolones was successfully achieved through pyrosequencing of the gyrA and parC quinolone-resistance determining regions. A strong correlation was found between quinolone resistance and mutations in gyrA codon 83 and/or in the parC gene (codons 80 or 84). Absence of QRDR mutations was associated with susceptibility to quinolones.
authors.affiliation: Defence Laboratories Department, Belgian Armed Forces, Brussels, Belgium.
authors.forename: Yann
authors.initials: Y
authors.lastname: Deccache
authors.forename: Léonid M
authors.initials: LM
authors.lastname: Irenge
authors.forename: Encho
authors.initials: E
authors.lastname: Savov
authors.forename: Mihaela
authors.initials: M
authors.lastname: Ariciuc
authors.forename: Alexandra
authors.initials: A
authors.lastname: Macovei
authors.forename: Angelina
authors.initials: A
authors.lastname: Trifonova
authors.forename: Ivanka
authors.initials: I
authors.lastname: Gergova
authors.forename: Jérôme
authors.initials: J
authors.lastname: Ambroise
authors.forename: Raymond
authors.initials: R
authors.lastname: Vanhoof
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Gala
mesh_terms: D000151:Acinetobacter Infections / Q000382:microbiology*; D040981:Acinetobacter baumannii / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D027081:DNA Gyrase / Q000235:genetics; D027101:DNA Topoisomerase IV / Q000235:genetics; D024881:Drug Resistance, Bacterial; D006801:Humans; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009154:Mutation; D011804:Quinolines / Q000494:pharmacology*; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D001426:Bacterial Proteins; D011804:Quinolines; D027101:DNA Topoisomerase IV; D027081:DNA Gyrase