title: Development of a series of gateway binary vectors possessing a tunicamycin resistance gene as a marker for the transformation of Arabidopsis thaliana.
abstract: We made two series of Gateway binary vectors, pGWBs and R4pGWBs, possessing a UDP-N-acetylglucosamine: dolichol phosphate N-acetylglucosamine-1-P transferase (GPT) gene driven by the nopaline synthase promoter (Pnos) as a tunicamycin resistance marker for the transformation of Arabidopsis thaliana. The reporters and tags employed in this system are sGFP, GUS, LUC, EYFP, ECFP, G3GFP, mRFP, TagRFP, 6xHis, FLAG, 3xHA, 4xMyc, 10xMyc, GST, T7, and TAP. Selection of transformants was successful on plates containing 0.15 mg/L of tunicamycin. These vectors were compatible with existing pGWB and R4pGWB vectors for kanamycin, hygromycin B, and BASTA® selection, and are useful new tools for making transgenic Arabidopsis.
authors.affiliation: Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Shinya
authors.initials: S
authors.lastname: Nakamura
authors.forename: Makoto
authors.initials: M
authors.lastname: Kawamukai
authors.forename: Nozomu
authors.initials: N
authors.lastname: Koizumi
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Nakagawa
mesh_terms: D017360:Arabidopsis / Q000187:drug effects* / Q000235:genetics*; D015415:Biomarkers / Q000378:metabolism; D004351:Drug Resistance / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics*; D014170:Transformation, Genetic / Q000187:drug effects* / Q000235:genetics*; D014415:Tunicamycin / Q000494:pharmacology*
chemical_list: D015415:Biomarkers; D014415:Tunicamycin