title: Characterization of a mannose-binding lectin from channel catfish (Ictalurus punctatus).
abstract: Mannose-binding lectin (MBL) is an important component of innate immunity capable of activating the lectin pathway of the complement system. A MBL gene was isolated from channel catfish (Ictalurus punctatus). The deduced protein contains a canonical collagen-like domain, a carbohydrate recognition domain (CRD), and a neck region similar to mammalian mannose-binding lectin. The catfish mannose-binding lectin CRD contains the EPN motif shown previously to mediate mannose specificity. The catfish mannose-binding lectin showed 30-43% identity with MBL protein sequences of rainbow trout, zebrafish, common carp, and goldfish, and 33-35% identity with sequences of mammalian species. In this study, while liver was the predominant source of mannose-binding lectin gene expression in healthy tissues, mannose-binding lectin expression in spleen rose sharply following challenge with a Gram-negative bacterium.
authors.affiliation: School of Life Science, East China Normal University, Shanghai 200062, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.forename: Eric
authors.initials: E
authors.lastname: Peatman
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.forename: Donghong
authors.initials: D
authors.lastname: Niu
authors.forename: Tingting
authors.initials: T
authors.lastname: Feng
authors.forename: Huseyin
authors.initials: H
authors.lastname: Kucuktas
authors.forename: Geoff
authors.initials: G
authors.lastname: Waldbieser
authors.forename: Liqiao
authors.initials: L
authors.lastname: Chen
authors.forename: Zhanjiang
authors.initials: Z
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000378:metabolism; D003001:Cloning, Molecular; D020610:Edwardsiella ictaluri; D004756:Enterobacteriaceae Infections / Q000276:immunology / Q000378:metabolism / Q000662:veterinary; D005753:Gastric Mucosa / Q000378:metabolism; D005786:Gene Expression Regulation / Q000276:immunology; D060226:Head Kidney / Q000378:metabolism; D007059:Ictaluridae / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D037601:Mannose-Binding Lectin / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000378:metabolism; D010802:Phylogeny; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012867:Skin / Q000378:metabolism; D013154:Spleen / Q000378:metabolism
chemical_list: D037601:Mannose-Binding Lectin