title: Mariniflexile aquimaris sp. nov., isolated from seawater, and emended description of the genus Mariniflexile Nedashkovskaya et al. 2006.
abstract: A Gram-staining-negative, non-flagellated, non-gliding and rod-shaped bacterial strain, designated HWR-17(T), was isolated from seawater of the Yellow Sea in Korea. Strain HWR-17(T) grew optimally at pH 7.0-8.0, at 30 °C and in the presence of 2% (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain HWR-17(T) clustered with the two Mariniflexile species in the family Flavobacteriaceae, exhibiting 16S rRNA gene sequence similarity of 97.1-97.2% to their type strains and less than 95.7% sequence similarity to other members of the family Flavobacteriaceae. Strain HWR-17(T) contained MK-6 as the predominant menaquinone and iso-C(15:0) as the major fatty acid. The polar lipid profile of strain HWR-17(T) contained phosphatidylethanolamine, an unidentified aminolipid and four unidentified lipids. The DNA G+C content of strain HWR-17(T) was 35.7 mol% and it exhibited 11 and 10% DNA-DNA relatedness, respectively, with Mariniflexile gromovii KCTC 12570(T) and Mariniflexile fucanivorans DSM 18792(T). The phylogenetic and genetic distinctiveness and differential phenotypic properties revealed that strain HWR-17(T) is distinguishable from the two recognized Mariniflexile species. On the basis of the data presented, strain HWR-17(T) is considered to represent a novel species of the genus Mariniflexile, for which the name Mariniflexile aquimaris sp. nov. is proposed. The type strain is HWR-17(T) (=KCTC 23346(T) =CCUG 60529(T)). An emended description of the genus Mariniflexile is also proposed.
authors.affiliation: University of Science and Technology (UST), 113 Gwahangno, Yuseong, Daejeon, Republic of Korea.|Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejeon, Republic of Korea.
authors.forename: Yong-Taek
authors.initials: YT
authors.lastname: Jung
authors.affiliation: University of Science and Technology (UST), 113 Gwahangno, Yuseong, Daejeon, Republic of Korea.|Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejeon, Republic of Korea.
authors.forename: Ji-Hoon
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejeon, Republic of Korea.
authors.forename: Tae-Kwang
authors.initials: TK
authors.lastname: Oh
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.|Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejeon, Republic of Korea.
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Yoon
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D005959:Glucosidases / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D007723:Korea; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature; D024482:Vitamin K 2 / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D012965:Sodium Chloride; D005959:Glucosidases