title: Elevation of HPV-18 and HPV-16 DNA in the plasma of patients with advanced cervical cancer.
abstract: OBJECTIVE
  Cervical cancer (CC) is one of the main problems in women's health in which the pathologic role of the human papilloma virus, HPV, is undeniable. Molecular methods have shown viral DNA in affected tissues, related to the disease progression.
  
  
  METHODS
  We here studied 100 patients with abnormal Pap test results. HPV DNA loads in the plasma samples were measured by quantitative real time PCR, using specific primers and probes for the E6 genes of HPV types 16, 18, 33 and 52. Another 50 women with no obvious malignancy were enrolled as controls.
  
  
  RESULTS
  Pathological studies revealed 81 patients with CC and 19 with cervical intraepithelial neoplasia. Only 19 of the cancer patients (15 with squamous cell carcinomas and 4 with adenocarcinomas) had detectable genetic material of HPV-16 (N = 4) and HPV-18 (N = 15) in their plasma; genetic material of other types was absent. HPV DNA copies increased with advanced disease in both types. Significantly smaller amounts of HPV DNA of types 16 and/or 18 were detected in the plasma of 16% of the controls while other types were negative.
  
  
  CONCLUSIONS
  The evidence of HPV DNA of high risk types in the plasma of women with CCs strongly emphasizes the necessity of more longitudinal comprehensive studies to determine its role as a possible biomarker in cervical cancer.
authors.affiliation: Shiraz Institute for Cancer Research, Iran. agahim@sums.ac.ir
authors.forename: Mansooreh
authors.initials: M
authors.lastname: Jaberipour
authors.forename: Alamtaj
authors.initials: A
authors.lastname: Samsami
authors.forename: Farahnaz
authors.initials: F
authors.lastname: Sahraiian
authors.forename: Talieh
authors.initials: T
authors.lastname: Kazerooni
authors.forename: Masoomeh
authors.initials: M
authors.lastname: Hashemi
authors.forename: Abbas
authors.initials: A
authors.lastname: Ghaderi
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Habibagahi
mesh_terms: D000230:Adenocarcinoma / Q000097:blood / Q000821:virology; D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000097:blood / Q000821:virology; D004279:DNA, Viral / Q000097:blood*; D018450:Disease Progression; D005260:Female; D052162:Human papillomavirus 16 / Q000235:genetics*; D052161:Human papillomavirus 18 / Q000235:genetics*; D006801:Humans; D008875:Middle Aged; D009367:Neoplasm Staging; D030361:Papillomavirus Infections / Q000097:blood / Q000821:virology*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D012189:Retrospective Studies; D002583:Uterine Cervical Neoplasms / Q000097:blood / Q000821:virology*; D019562:Viral Load; D055815:Young Adult; D002578:Uterine Cervical Dysplasia / Q000097:blood / Q000821:virology
chemical_list: D004279:DNA, Viral