title: Synergistic interactions between Glomus mosseae and Bradyrhizobium japonicum in enhancing proton release from nodules and hyphae.
abstract: Soybean (Glycine max L. Merr.) seedlings were inoculated with Glomus mosseae (GM) and Bradyrhizobium japonicum (BJ) together or separately to study the effect of interactions on net H(+) effluxes of nodules or extraradical hyphae by in vivo vibrating electrode techniques. GM promoted three-fold the H(+) effluxes of nodules on mycorrhizal lateral roots and BJ increased eight-fold the net H(+) effluxes of hyphae developing in the vicinity of nodules on lateral roots. Increments in plant P content were positively and linearly correlated with the net H(+) efflux of nodules and hyphae. It is concluded that increased H(+) effluxes of nodules resulted from enhanced nitrogenase activities induced by the presence of the AM fungus in lateral roots. The results point to additive effects of interactions between mycorrhizal fungi and rhizobia in increasing the extent of acidification of the "nodulesphere" and the hyposphere.
authors.affiliation: Key Laboratory of Plant-Soil Interactions, Ministry of Education, College of Resource and Environmental Science, China Agricultural University, Beijing, China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Ding
authors.forename: Xinhua
authors.initials: X
authors.lastname: Sui
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Junhua
authors.initials: J
authors.lastname: Gao
authors.forename: Xinhua
authors.initials: X
authors.lastname: He
authors.forename: Fusuo
authors.initials: F
authors.lastname: Zhang
authors.forename: Juncheng
authors.initials: J
authors.lastname: Yang
authors.forename: Gu
authors.initials: G
authors.lastname: Feng
mesh_terms: D020369:Bradyrhizobium / Q000502:physiology*; D055137:Glomeromycota / Q000502:physiology*; D025301:Hyphae / Q000502:physiology; D056265:Microbial Interactions / Q000502:physiology*; D038821:Mycorrhizae / Q000502:physiology*; D009584:Nitrogen / Q000378:metabolism; D009586:Nitrogen Fixation; D009591:Nitrogenase / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism; D018517:Plant Roots / Q000382:microbiology; D011522:Protons; D058441:Rhizosphere; D053204:Root Nodules, Plant / Q000201:enzymology / Q000382:microbiology / Q000502:physiology; D036226:Seedlings / Q000382:microbiology / Q000502:physiology; D013025:Glycine max / Q000382:microbiology* / Q000502:physiology
chemical_list: D010710:Phosphates; D011522:Protons; D009591:Nitrogenase; D009584:Nitrogen