title: Crystallization and preliminary X-ray analysis of the bacillaene synthase trans-acting acyltransferase PksC.
abstract: The antibiotic bacillaene is biosynthesized in Bacillus subtilis by a hybrid type 1 modular polyketide synthase/nonribosomal peptide synthetase of the trans-acyltransferase (trans-AT) class. Within this system, the essential acyl-group loading activity is provided by the action of three free-standing trans-acting acyltransferases. Here, the recombinant expression, purification and crystallization of the bacillaene synthase trans-acting acyltransferase PksC are reported. A diffraction data set has been collected from a single PksC crystal to 1.44 Å resolution and the crystal was found to belong to the orthorhombic space group P2(1)2(1)2(1).
authors.affiliation: Institute for Cell and Molecular Biosciences, Faculty of Medical Sciences, Newcastle University, Framlington Place, Newcastle-upon-Tyne NE2 4HH, England.
authors.forename: Fiona
authors.initials: F
authors.lastname: Cuskin
authors.forename: Alexandra S
authors.initials: AS
authors.lastname: Solovyova
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Lewis
authors.forename: Paul R
authors.initials: PR
authors.lastname: Race
mesh_terms: D000217:Acyltransferases / Q000737:chemistry*; D001412:Bacillus subtilis / Q000201:enzymology*; D003460:Crystallization; D018360:Crystallography, X-Ray
chemical_list: D000217:Acyltransferases