title: Inflammatory cytokine and matrix metalloproteinase expression induced by collagen-coated and uncoated polypropylene meshes in a rat model.
abstract: OBJECTIVE
  The objective of the study was to compare the influence of collagen-coated vs uncoated polypropylene meshes on the expression of genes critical for wound healing.
  
  
  METHODS
  In 54 rats, abdominal wall defects were created, repaired by polypropylene sutures, and covered by an overlay of coated polypropylene (n = 20), uncoated polypropylene (n = 18), or no mesh (n = 16). Explants were harvested 7 or 90 days after repair and divided for histological, immunohistochemical, and messenger ribonucleic acid (mRNA) analyses. Real-time quantitative polymerase chain reaction arrays were used to profile the expression of 84 genes at the tissue-mesh interface.
  
  
  RESULTS
  One week after implantation, coated mesh elicited a slightly greater inflammatory response and increased mRNA expression of 4 proinflammatory cytokines compared with uncoated mesh. Both materials, however, induced a comparable expression of cytokines and matrix metalloproteinases relative to suture repair 90 days after implantation.
  
  
  CONCLUSIONS
  Collagen-coated polypropylene mesh induces elevated inflammatory cytokine expression compared with uncoated mesh early in the healing process, but the response to both meshes is similar 90 days after implantation.
authors.affiliation: Department of Clinical Investigation, Tripler Army Medical Center, Honolulu, HI, USA.
authors.forename: Lisa M
authors.initials: LM
authors.lastname: Pierce
authors.forename: Jennifer R
authors.initials: JR
authors.lastname: Asarias
authors.forename: Phuoc T
authors.initials: PT
authors.lastname: Nguyen
authors.forename: Jamie R
authors.initials: JR
authors.lastname: Mings
authors.forename: Alan P
authors.initials: AP
authors.lastname: Gehrich
mesh_terms: D034861:Abdominal Wall / Q000473:pathology / Q000601:surgery; D000818:Animals; D001672:Biocompatible Materials / Q000378:metabolism; D003094:Collagen / Q000378:metabolism*; D016207:Cytokines / Q000235:genetics*; D020869:Gene Expression Profiling; D007249:Inflammation / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D008297:Male; D020782:Matrix Metalloproteinases / Q000235:genetics*; D011126:Polypropylenes; D051381:Rats; D013526:Surgical Mesh; D014945:Wound Healing / Q000235:genetics
chemical_list: D001672:Biocompatible Materials; D016207:Cytokines; D011126:Polypropylenes; D003094:Collagen; D020782:Matrix Metalloproteinases