title: Zinc(II) and PPi selective fluorescence OFF-ON-OFF functionality of a chemosensor in physiological conditions.
abstract: A fluorescent chemosensor based on a quinoline derivative, L(2) (OFF state), selectively senses Zn(2+) by effective chelate-enhanced fluorescence (ON state), which further shows selectivity toward PPi over competing anions like Pi, AMP, and ATP via fluorescence quenching (OFF state) in a 100% aqueous HEPES buffer (pH 7.4). A plausible mode for the selective binding of PPi to 1 has been demonstrated by quantum mechanical density functional theory calculations and high-resolution mass spectrometry analysis.
authors.affiliation: Department of Inorganic Chemistry, Indian Association for the Cultivation of Science (IACS), 2A and 2B Raja S. C. Mullick Road, Kolkata 700032, India.
authors.forename: I
authors.initials: I
authors.lastname: Ravikumar
authors.forename: Pradyut
authors.initials: P
authors.lastname: Ghosh
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002021:Buffers; D018360:Crystallography, X-Ray; D011756:Diphosphates / Q000032:analysis* / Q000378:metabolism; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D011804:Quinolines / Q000737:chemistry / Q000378:metabolism*; D012996:Solutions; D013050:Spectrometry, Fluorescence; D014867:Water / Q000737:chemistry; D015032:Zinc / Q000032:analysis* / Q000378:metabolism
chemical_list: D002021:Buffers; D011756:Diphosphates; D005456:Fluorescent Dyes; D011804:Quinolines; D012996:Solutions; D014867:Water; D015032:Zinc