title: Identification and evaluation of an outer membrane protein OmpU from a pathogenic Vibrio harveyi isolate as vaccine candidate in turbot (Scophthalmus maximus).
abstract: OBJECTIVE
  The main aims of this study were to clone and express a new outer membrane protein U (OmpU) from a pathogenic Vibrio harveyi SF-1 and investigate its immune efficiency as a vaccine candidate against V. harveyi infection in turbot (Scophthalmus maximus).
  
  
  RESULTS
  In this study, a new gene, ompU was cloned from the genomic DNA of pathogenic V. harveyi SF-1. The ompU gene encoded a 35 kDa protein, which was purified by Ni-NTA His-Bind Resin column. A DNA vaccine was constructed by inserting ompU gene into pEGFP-N1 plasmid. Turbot were injected intramuscularly with the purified OmpU protein and the recombinant pEGFP-N1/ompU plasmid, respectively. The fish vaccinated with the purified OmpU protein were completely protected with a relative per cent of survival (RPS) of 100% against pathogenic V. harveyi infection. Efficient protection was also found in the pEGFP-N1/ompU vaccinated group, with a RPS of 51.4%. Significant specific antibody responses were detected in the vaccinated turbot by indirect enzyme-linked immunosorbent assay.
  
  
  CONCLUSIONS
  A new OmpU was cloned and expressed. Both OmpU protein vaccine and DNA vaccine showed good immune protections in turbot.
  
  
  CONCLUSIONS
  The OmpU was identified to be a new effective vaccine candidate and could be used as subunit vaccine and DNA vaccine for disease control caused by pathogenic V. harveyi.
authors.affiliation: Department of Marine Biology, College of Marine Life Sciences, Ocean University of China, Qingdao, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Wang
authors.forename: J
authors.initials: J
authors.lastname: Chen
authors.forename: R
authors.initials: R
authors.lastname: Liu
authors.forename: J
authors.initials: J
authors.lastname: Jia
mesh_terms: D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology; D001428:Bacterial Vaccines / Q000276:immunology*; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay; D005393:Fish Diseases / Q000276:immunology* / Q000382:microbiology / Q000517:prevention & control*; D005412:Flatfishes; D015870:Gene Expression; D049452:Green Fluorescent Proteins / Q000235:genetics; D010957:Plasmids; D014158:Transcription, Genetic; D019444:Vaccines, DNA / Q000276:immunology; D014733:Vibrio / Q000737:chemistry* / Q000235:genetics* / Q000502:physiology; D014735:Vibrio Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D019444:Vaccines, DNA; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins