title: Spectral domain fluorescence coherence phase microscopy.
abstract: Spectral domain phase microscopy (SDPM) has been reported in the literature as a functional extension to low-coherence interferometry, which enables nanoscale measurement of a scatter's displacement. The signal in SDPM is generated from structural images that lack molecular specificity. This paper investigates the expansion of phase analysis to fluorescence self-interference signals to provide functional information about a sample. Spectral domain fluorescence coherence phase microscopy is demonstrated for nanoscale resolution motion detection of fluorescent particles with a signal-to-noise ratio limited resolution of ~10 nm. This paper demonstrates the feasibility of combining phase processing with fluorescence self-interference, which may be useful for future applications such as cell rheology.
authors.affiliation: School of Engineering Science, Simon Fraser University, 8888 University Drive, Burnaby, Canada, V5A 1S6.
authors.forename: Andra
authors.initials: A
authors.lastname: St Quintin
authors.forename: Lukas-Karim
authors.initials: LK
authors.lastname: Merhi
authors.forename: Marinko V
authors.initials: MV
authors.lastname: Sarunic
mesh_terms: D000818:Animals; D016477:Artifacts; D004867:Equipment Design; D005456:Fluorescent Dyes / Q000494:pharmacology; D006801:Humans; D008027:Light; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D008858:Microscopy, Phase-Contrast / Q000295:instrumentation / Q000379:methods*; D008863:Microspheres; D055095:Optics and Photonics; D011108:Polymers / Q000737:chemistry; D012212:Rheology / Q000379:methods; D012542:Scattering, Radiation; D013053:Spectrophotometry / Q000379:methods; D013997:Time Factors
chemical_list: D005456:Fluorescent Dyes; D011108:Polymers