title: The glycosylphosphatidylinositol-linked aspartyl protease Yps1 is transcriptionally regulated by the calcineurin-Crz1 and Slt2 MAPK pathways in Candida glabrata.
abstract: In the pathogenic fungus Candida glabrata, the YPS1 gene, which encodes a glycosylphosphatidylinositol-linked aspartyl protease, is required for cell wall integrity and virulence. Although the expression of YPS1 has been studied in Saccharomyces cerevisiae, the transcriptional regulation of this gene in C. glabrata is not well understood. Here, we report that C. glabrata Yps1 is required for cell growth at elevated temperatures, and that the heat-induced expression of YPS1 is regulated predominantly by the calcineurin-Crz1 pathway and partially by the Slt2 MAPK pathway. Although a total of 11 YPS genes are present in the C. glabrata genome, the loss of transcriptional induction in a calcineurin mutant was observed only for YPS1. The results of a YPS1 promoter-lacZ reporter assay using a series of constructs with mutated promoter elements indicated that the transcription factor Crz1 binds to multiple sites in the promoter region of YPS1. To date, as none of the putative Crz1 targets in C. glabrata have been characterized using a Δcrz1 mutant, monitoring the expression of YPS1 represents an effective method for measuring the activity of the calcineurin-Crz1 signaling pathway in this fungus.
authors.affiliation: Department of Molecular Microbiology and Immunology, Nagasaki University School of Medicine, Japan. taiga-m@nagasakiu.ac.jp
authors.forename: Taiga
authors.initials: T
authors.lastname: Miyazaki
authors.forename: Koichi
authors.initials: K
authors.lastname: Izumikawa
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Yamauchi
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Inamine
authors.forename: Yohsuke
authors.initials: Y
authors.lastname: Nagayoshi
authors.forename: Tomomi
authors.initials: T
authors.lastname: Saijo
authors.forename: Masafumi
authors.initials: M
authors.lastname: Seki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kakeya
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Katsunori
authors.initials: K
authors.lastname: Yanagihara
authors.forename: Yoshitsugu
authors.initials: Y
authors.lastname: Miyazaki
authors.forename: Akira
authors.initials: A
authors.lastname: Yasuoka
authors.forename: Shigeru
authors.initials: S
authors.lastname: Kohno
mesh_terms: D057055:Aspartic Acid Proteases / Q000235:genetics* / Q000378:metabolism; D019703:Calcineurin / Q000235:genetics* / Q000378:metabolism*; D041221:Candida glabrata / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D002177:Candidiasis / Q000382:microbiology*; D002473:Cell Wall / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D005816:Genetic Complementation Test; D017261:Glycosylphosphatidylinositols; D006358:Hot Temperature; D009154:Mutation; D011401:Promoter Regions, Genetic; D012331:RNA, Fungal / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*; D014774:Virulence; D001616:beta-Galactosidase
chemical_list: D005656:Fungal Proteins; D017261:Glycosylphosphatidylinositols; D012331:RNA, Fungal; D012333:RNA, Messenger; D014157:Transcription Factors; D019703:Calcineurin; D001616:beta-Galactosidase; D057055:Aspartic Acid Proteases