title: Detection of norovirus, sapovirus, and human astrovirus in fecal specimens using a multiplex reverse transcription-PCR with fluorescent dye-labeled primers.
abstract: We applied a multiplex reverse transcription-PCR with fluorescent dye-labeled primers (fluorescent multiplex RT-PCR) for noroviruses (NoV), sapovirus (SaV), and human astrovirus (HAstV) to diagnose 71 outbreaks of acute gastroenteritis during July 2007 and May 2010 in Hiroshima prefecture. In this assay, the green, red, yellow, and blue fluorescence for NoV genogroup I, NoV genogroup II, SaV, and HAstV, respectively, were indicated on an agarose gel under ultraviolet light. In 61 virus-positive outbreaks confirmed by fluorescent multiplex RT-PCR, detection rates of outbreaks for NoVs, SaV, and HAstV were 96.7%, 3.3%, and 0%, respectively.
authors.affiliation: Center for Public Health and Environment, Hiroshima Prefectural Technology Research Institute, Minami-ku, Hiroshima, Japan. n-shigemoto81161@pref.hiroshima.lg.jp
authors.forename: Naoki
authors.initials: N
authors.lastname: Shigemoto
authors.forename: Shinji
authors.initials: S
authors.lastname: Fukuda
authors.forename: Yukie
authors.initials: Y
authors.lastname: Tanizawa
authors.forename: Masaru
authors.initials: M
authors.lastname: Kuwayama
authors.forename: Sachiko
authors.initials: S
authors.lastname: Ohara
authors.forename: Masato
authors.initials: M
authors.lastname: Seno
mesh_terms: D017931:DNA Primers; D004196:Disease Outbreaks; D005243:Feces / Q000821:virology*; D005456:Fluorescent Dyes; D005759:Gastroenteritis / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D006801:Humans; D007564:Japan; D001257:Mamastrovirus / Q000235:genetics / Q000302:isolation & purification*; D029322:Norovirus / Q000235:genetics / Q000302:isolation & purification*; D012367:RNA, Viral / Q000032:analysis / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D029341:Sapovirus / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D017931:DNA Primers; D005456:Fluorescent Dyes; D012367:RNA, Viral