title: Isolation of inflammatory cells from human tumours.
abstract: Inflammatory cells are present in many tumours, and understanding their function is of increasing importance, particularly to studies of tumour immunology. The tumour-infiltrating leukocytes encompass a variety of cell types, e.g. T lymphocytes, macrophages, dendritic cells, NK cells, and mast cells. Choice of the isolation method greatly depends on the tumour type and the leukocyte subset of interest, but the protocol usually includes tissue disaggregation and cell enrichment. We recommend density centrifugation for initial enrichment, followed by specific magnetic bead negative or positive panning with leukocyte and tumour cell selective antibodies.
authors.affiliation: Division of Infection, Inflammation and Immunity, University of Southampton School of Medicine, Southampton, UK. marta.polak@gmail.com
authors.forename: Marta E
authors.initials: ME
authors.lastname: Polak
mesh_terms: D000938:Antigen-Presenting Cells / Q000166:cytology / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000150:complications / Q000276:immunology / Q000473:pathology; D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient; D017364:Collagenases / Q000378:metabolism; D006801:Humans; D007249:Inflammation / Q000150:complications / Q000276:immunology / Q000473:pathology*; D007962:Leukocytes / Q000166:cytology / Q000378:metabolism; D008280:Magnetics; D009369:Neoplasms / Q000150:complications / Q000276:immunology / Q000473:pathology*; D013535:Suspensions; D013601:T-Lymphocytes / Q000166:cytology / Q000378:metabolism
chemical_list: D013535:Suspensions; D017364:Collagenases