title: Expression analysis of homeobox genes in leukemia/lymphoma cell lines.
abstract: Homeobox genes code for transcription factors which have a strong impact on cellular behavior, including differentiation, proliferation, and survival. Therefore, upon deregulation these genes may turn into oncogenes, contributing substantially to cancerogenesis. Among hematopoietic malignancies, including leukemias and lymphomas, several homeo-oncogenes have been described. Many of them have been identified in hematopoietic cell lines, which serve as useful tools for oncogene hunting and characterization. Here, we describe molecular methods for analysis and quantification of dysregulated homeobox gene expression in leukemia/lymphoma cell lines.
authors.affiliation: DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen, Braunschweig, Germany. sna@dsmz.de
authors.forename: Stefan
authors.initials: S
authors.lastname: Nagel
authors.forename: Hans G
authors.initials: HG
authors.lastname: Drexler
mesh_terms: D001483:Base Sequence; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D005801:Genes, Homeobox / Q000235:genetics*; D006801:Humans; D007938:Leukemia / Q000235:genetics / Q000473:pathology*; D008223:Lymphoma / Q000235:genetics / Q000473:pathology*; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000235:genetics; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
chemical_list: D018076:DNA, Complementary; D009841:Oligonucleotides; D012313:RNA