title: Direct assessments of the antioxidant effects of the novel collagen peptide on reactive oxygen species using electron spin resonance spectroscopy.
abstract: In the present study, we evaluated the antioxidant effects of a pepsin-treated novel collagen peptide (P-NCP) on reactive oxygen species (ROS) such as hydroxyl radical (HO(•)), superoxide anion radical (O(2)(•-)), and singlet oxygen ((1)O(2)), and the effects on cell viability after ultraviolet ray (UV) irradiation of human fibroblasts. We confirmed, using electron spin resonance, that P-NCP directly inhibited HO(•) and (1)O(2). Furthermore, addition of P-NCP to fibroblasts inhibited cell death induced by UVA (400-315 nm) irradiation in a dose-dependent manner. In addition, the antioxidant effect on (1)O(2) was observed in the peptide fractions rich in Gly, Pro, Hyp, Glu, Ala, and Arg. We found that Gly, Hyp, Glu, and Ala directly scavenged (1)O(2). These results indicated that a peptide sequence including Gly, Hyp, Glu, and Ala could play a key role in the antioxidant effects of P-NCP on (1)O(2). It was suggested that P-NCP can inhibit photo-aging related to ROS owing to its antioxidant effects.
authors.affiliation: Division of Pharmacology and ESR Laboratories, Department of Clinical Care Medicine, Kanagawa Dental College, Yokosuka, Kanagawa 238-8580, Japan.
authors.forename: Kyo
authors.initials: K
authors.lastname: Kobayashi
authors.forename: Yojiro
authors.initials: Y
authors.lastname: Maehata
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Kawamura
authors.forename: Masashi
authors.initials: M
authors.lastname: Kusubata
authors.forename: Shunji
authors.initials: S
authors.lastname: Hattori
authors.forename: Keisuke
authors.initials: K
authors.lastname: Tanaka
authors.forename: Chihiro
authors.initials: C
authors.lastname: Miyamoto
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Yoshino
authors.forename: Ayaka
authors.initials: A
authors.lastname: Yoshida
authors.forename: Satoko
authors.initials: S
authors.lastname: Wada-Takahashi
authors.forename: Tomoko
authors.initials: T
authors.lastname: Komatsu
authors.forename: Shun-Suke
authors.initials: SS
authors.lastname: Takahashi
authors.forename: Masaichi-Chang-Il
authors.initials: MC
authors.lastname: Lee
mesh_terms: D000596:Amino Acids / Q000032:analysis / Q000737:chemistry; D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D002417:Cattle; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002850:Chromatography, Gel; D024042:Collagen Type I / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D006868:Hydrolysis; D008297:Male; D010446:Peptide Fragments / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D011492:Protein Hydrolysates / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors; D026082:Singlet Oxygen / Q000037:antagonists & inhibitors; D012867:Skin / Q000187:drug effects / Q000528:radiation effects; D015595:Skin Aging / Q000187:drug effects; D014466:Ultraviolet Rays / Q000009:adverse effects
chemical_list: D000596:Amino Acids; D000975:Antioxidants; D024042:Collagen Type I; D016166:Free Radical Scavengers; D010446:Peptide Fragments; D011492:Protein Hydrolysates; D017382:Reactive Oxygen Species; D026082:Singlet Oxygen