title: Nano-C(60) : a novel, effective, fluorescent sensing platform for biomolecular detection.
abstract: Water-soluble nano-C(60) can serve as a novel, effective, fluorescent sensing platform for biomolecular detection with high sensitivity and selectivity. In this paper, fluorescent detection of DNA and thrombin via nano-C(60) is demonstrated for the first time. The principle of the assay lies in the fact that the adsorption of the fluorescently labeled single-stranded DNA (ssDNA) probe by nano-C(60) leads to substantial fluorescence quenching. In the presence of a target, the biomolecular mutual interaction suppresses this quenching, signaling the existence of the target. This sensing system rivals graphene oxide but is superior to other carbon-structure-based systems. The present method can also achieve multiplex DNA detection and withstand the interference from human blood serum.
authors.affiliation: Changchun Institute of Applied Chemistry, Changchun 130022, Jilin, People's Republic of China.
authors.forename: Hailong
authors.initials: H
authors.lastname: Li
authors.forename: Yingwei
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yonglan
authors.initials: Y
authors.lastname: Luo
authors.forename: Xuping
authors.initials: X
authors.lastname: Sun
mesh_terms: D020137:Base Pair Mismatch / Q000235:genetics; D001483:Base Sequence; D004247:DNA / Q000032:analysis*; D037741:Fullerenes / Q000737:chemistry*; D006801:Humans; D007700:Kinetics; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013917:Thrombin / Q000032:analysis*
chemical_list: D037741:Fullerenes; D004247:DNA; D013917:Thrombin