title: Modification of the deoxy-myoglobin/carbonmonoxy-myoglobin UV-vis assay for reliable determination of CO-release rates from organometallic carbonyl complexes.
abstract: The deoxy-myoglobin (deoxy-Mb)/carbonmonoxy-myoglobin (Mb-CO) UV-vis assay is the principal method used for quantifying the rates of CO release from CO-releasing molecules (CO-RMs) that might possess therapeutic benefits. Some issues emerge when the Mb-CO assay is utilized for testing CO-RMs with novel structures, which are comprehensively discussed here for the first time. Two methods for processing raw UV-vis spectroscopic data generated from the assay are presented in this paper.
authors.affiliation: Department of Chemistry, University of York, Heslington, York.
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: Atkin
authors.forename: Jason M
authors.initials: JM
authors.lastname: Lynam
authors.forename: Benjamin E
authors.initials: BE
authors.lastname: Moulton
authors.forename: Philip
authors.initials: P
authors.lastname: Sawle
authors.forename: Roberto
authors.initials: R
authors.lastname: Motterlini
authors.forename: Nicola M
authors.initials: NM
authors.lastname: Boyle
authors.forename: Mary T
authors.initials: MT
authors.lastname: Pryce
authors.forename: Ian J S
authors.initials: IJ
authors.lastname: Fairlamb
mesh_terms: D002248:Carbon Monoxide / Q000032:analysis*; D056831:Coordination Complexes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D009211:Myoglobin / Q000737:chemistry*; D009942:Organometallic Compounds / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
chemical_list: D056831:Coordination Complexes; D009211:Myoglobin; D009942:Organometallic Compounds; C018013:carboxymyoglobin; C055547:deoxymyoglobin; D002248:Carbon Monoxide