title: The preparation of bovine serum albumin surface-imprinted superparamagnetic polymer with the assistance of basic functional monomer and its application for protein separation.
abstract: Currently, small proteins imprinting are more reported since large proteins molecular imprinting faces challenge due to their bulk size and complex structure. In this work, bovine serum albumin (BSA) surface-imprinted magnetic polymer was successfully synthesized based on atomic transfer radical polymerization (ATRP) method in the presence of common monomer (N-isopropylacrylamide) with the assistant of basic functional monomer (N-[3-(dimethylamino)propyl]-methacrylamide), which provides a achievable attempt for imprinting larger target proteins based on the ATPR with the mild reaction conditions. The BSA-imprinted polymer exhibited higher adsorption capacity and selectivity to BSA over the non-imprinted polymer. Competitive adsorption tests indicated the BSA-imprinted polymer had better selective adsorption and recognition properties to BSA in the mixture. The obtained BSA-imprinted polymer was applied to bovine serum, which also showed selectivity to BSA. In addition, a conventional aqueous two-phase solution of PEG/sulphate was used as elution for adsorbed BSA, which was compared with common NaCl elution.
authors.affiliation: School of Life Science, Beijing Institute of Technology, 5 South Zhongguancun Street, Beijing 100081, China.
authors.forename: Qing-Qing
authors.initials: QQ
authors.lastname: Gai
authors.forename: Feng
authors.initials: F
authors.lastname: Qu
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.forename: Yu-Kui
authors.initials: YK
authors.lastname: Zhang
mesh_terms: D000327:Adsorption; D000818:Animals; D002417:Cattle; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D054802:Molecular Imprinting / Q000379:methods*; D011092:Polyethylene Glycols; D011506:Proteins / Q000302:isolation & purification*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012965:Sodium Chloride; D013431:Sulfates
chemical_list: D011506:Proteins; D013431:Sulfates; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; D012965:Sodium Chloride; D052203:Ferrosoferric Oxide