title: Analysis of outer membrane vesicle protein involved in biofilm formation of Helicobacter pylori.
abstract: Helicobacter pylori is one of the most common causes of bacterial infection in humans. Infection with H. pylori is closely associated with gastritis and peptic ulcers and is a risk factor for gastric cancer and mucosa-associated lymphoid tissue lymphoma. H. pylori forms biofilms on glass surfaces at the air-liquid interface in in-vitro batch cultures. We previously reported that strain TK1402 showed a strong biofilm-forming ability in vitro. We also suggested the outer membrane vesicles (OMV) produced by strain TK1402 might be related to its biofilm forming ability. In the present study, we analyzed the protein profile of the OMV produced by strain TK1402 and found a unique 22-kDa protein in TK1402 OMV cultured for 2-3 days. In addition, this protein could not be detected in the OMVs produced by other H. pylori strains. These results suggest that the 22-kDa protein is involved in effective biofilm formation by strain TK1402.
authors.affiliation: Department of Infectious Disease, Kyorin University School of Medicine, 6-20-2 Shinkawa, Mitaka-shi, Tokyo 181-8611, Japan.
authors.forename: Hideo
authors.initials: H
authors.lastname: Yonezawa
authors.forename: Takako
authors.initials: T
authors.lastname: Osaki
authors.forename: Timothy
authors.initials: T
authors.lastname: Woo
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kurata
authors.forename: Cynthia
authors.initials: C
authors.lastname: Zaman
authors.forename: Fuhito
authors.initials: F
authors.lastname: Hojo
authors.forename: Tomoko
authors.initials: T
authors.lastname: Hanawa
authors.forename: Shuichi
authors.initials: S
authors.lastname: Kato
authors.forename: Shigeru
authors.initials: S
authors.lastname: Kamiya
mesh_terms: D001422:Bacterial Adhesion; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis*; D018441:Biofilms / Q000254:growth & development*; D016480:Helicobacter pylori / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D020543:Proteome / Q000032:analysis; D022142:Secretory Vesicles / Q000737:chemistry*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D020543:Proteome