title: Isolation, antiproliferation on tumor cell and immunomodulatory activity of BSP-I, a novel bursal peptide from chicken humoral immune system.
abstract: The bursa of Fabricius (BF) is acknowledged as central humoral immune organ unique to birds. Our purpose was to identify the potential function of a novel bursal-derived bioactive peptide. A bursal septpeptide (BSP-I), EPASGMM, first isolated from BF, reduced MCF and Hela tumor cells proliferation, and enhanced antitumor factor p53 luciferase activity and protein expression. Further, we found the significantly immune inducing function of BSP-I on antigen-specific immune response in BALB/c mice intraperitoneally immunized with inactivated avian influence virus (AIV, H(9)N(2) subtype) vaccine, including of enhancing the antibody (IgG, the isotypes IgG1 and IgG2a) production, and stimulating cytokines IL-4 and IFN-γ level, and inducing T cell immunophenotyping and lymphocyte proliferation. These results suggested that as the bioactive peptide from avian humoral immune system, various biological function of BSP-I may have far-reaching implication on immune system significance, which might provide novel insight on linking between humoral immune system and development of effective immunotherapeutic strategies for treating human cancers diseases.
authors.affiliation: Division of Key Lab of Animal Disease Diagnosis and Immunology of China's Department of Agriculture, Nanjing Agriculture University, Nanjing, China.
authors.forename: Xiuli
authors.initials: X
authors.lastname: Feng
authors.forename: Taoqing
authors.initials: T
authors.lastname: Liu
authors.forename: Fangquan
authors.initials: F
authors.lastname: Wang
authors.forename: Ruibing
authors.initials: R
authors.lastname: Cao
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xiang
authors.initials: X
authors.lastname: Mao
authors.forename: Puyan
authors.initials: P
authors.lastname: Chen
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D030161:Avian Proteins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000188:drug therapy / Q000473:pathology; D002060:Bursa of Fabricius / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D002645:Chickens / Q000276:immunology; D056148:Chromatography, Reverse-Phase; D004797:Enzyme-Linked Immunosorbent Assay; D015870:Gene Expression / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis* / Q000276:immunology; D007155:Immunologic Factors / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology*; D053127:Influenza A Virus, H9N2 Subtype / Q000187:drug effects / Q000254:growth & development / Q000276:immunology; D005585:Influenza in Birds / Q000188:drug therapy / Q000276:immunology; D007371:Interferon-gamma / Q000096:biosynthesis* / Q000276:immunology; D015847:Interleukin-4 / Q000096:biosynthesis* / Q000276:immunology; D008156:Luciferases / Q000032:analysis; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009842:Oligopeptides / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology*; D010455:Peptides / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism
chemical_list: D000906:Antibodies; D030161:Avian Proteins; D007074:Immunoglobulin G; D007155:Immunologic Factors; D009842:Oligopeptides; D010455:Peptides; D016159:Tumor Suppressor Protein p53; D015847:Interleukin-4; D007371:Interferon-gamma; D008156:Luciferases