title: Early gene response of human brain microvascular endothelial cells to Listeria monocytogenes infection.
abstract: The gene expression of human brain microvascular endothelial cells (HBMEC) in response to 4 h of infection by Listeria monocytogenes was analyzed. Four hours after infection, the expression of 456 genes of HBMEC had changed (p < 0.05). We noted that many active genes were involved in the formyl-methionyl-leucyl-phenylalanine pathway in infected HBMEC. In the upregulated genes, mRNA levels of interleukin-8 and interleukin-15 in infected cells increased according to microarray and real-time reverse transcription - PCR analyses. Since both cytokines are regarded as potent chemotactic factors, the results suggest that HBMEC are capable of recruiting cells of innate and adaptive immune responses during early L. monocytogenes infection.
authors.affiliation: Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762, USA.
authors.forename: Chinling
authors.initials: C
authors.lastname: Wang
authors.forename: Chung-Hsi
authors.initials: CH
authors.lastname: Chou
authors.forename: Charles
authors.initials: C
authors.lastname: Tseng
authors.forename: Xijin
authors.initials: X
authors.lastname: Ge
authors.forename: Lesya M
authors.initials: LM
authors.lastname: Pinchuk
mesh_terms: D001921:Brain / Q000098:blood supply; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000378:metabolism / Q000382:microbiology*; D004727:Endothelium / Q000166:cytology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D054884:Host-Pathogen Interactions / Q000235:genetics*; D006801:Humans; D019409:Interleukin-15 / Q000235:genetics / Q000378:metabolism; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism; D008089:Listeria monocytogenes / Q000472:pathogenicity*; D008088:Listeriosis / Q000235:genetics*; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D059467:Transcriptome
chemical_list: D019409:Interleukin-15; D016209:Interleukin-8; D009240:N-Formylmethionine Leucyl-Phenylalanine