title: Chromatin immunoprecipitation in fission yeast.
abstract: A tremendous amount of information regarding the nature and regulation of heterochromatin has emerged in the past 10 years. This rapid progress is largely due to the development of techniques such as chromatin immunoprecipitation or "ChIP," which allow analysis of chromatin structure. Further technological advances such as microarray analysis and, more recently, deep sequencing technologies, have made ChIP an even more powerful tool. ChIP allows the investigator to identify protein interactions and/or the presence of various chromatin modifications at specific genomic loci.
authors.affiliation: Feinberg School of Medicine, Northwestern University, Chicago, IL, USA. t-volpe2@northwestern.edu
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Volpe
authors.forename: Jessica
authors.initials: J
authors.lastname: Demaio
mesh_terms: D000906:Antibodies / Q000378:metabolism; D060565:Argonaute Proteins; D002843:Chromatin / Q000378:metabolism; D047369:Chromatin Immunoprecipitation; D016601:RNA-Binding Proteins / Q000378:metabolism; D012568:Schizosaccharomyces / Q000254:growth & development / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000378:metabolism
chemical_list: C481643:Ago1 protein, S pombe; D000906:Antibodies; D060565:Argonaute Proteins; D002843:Chromatin; D016601:RNA-Binding Proteins; D029702:Schizosaccharomyces pombe Proteins