title: P53 alterations show significant correlation with gene amplification and s-phase index in breast-cancer.
abstract: Correlation of p53 gene/protein alterations with incidence of oncogene amplification, a potential marker of prognosis, was evaluated in 26 fresh breast cancer samples. p53 gene was analyzed by SSCP and DNA sequencing while p53 protein status was investigated by immunohistochemistry (IH). Amplification of c-erbB2/neu, c-myc, N-ras, int-2, hst, PRAD-1 and EGFR genes was studied by slot blot and in situ hybridizations. p53 alterations were found in 31% cases by SSCP and 42% by IH; gene amplification was detected in 27% cases. p53 gene alterations correlated significantly with gene amplification (p=0.006) and also with higher S-phase index (p=0.026), aneuploidy (p=0.026) and negative progesterone receptor status (p=0.043).
authors.affiliation: UNIV TEXAS, MD ANDERSON CANC CTR, DIV LAB MED, HOUSTON, TX 77030 USA. UNIV TEXAS, MD ANDERSON CANC CTR, DIV PATHOL, HOUSTON, TX 77030 USA. UNIV TEXAS, MD ANDERSON CANC CTR, DEPT BREAST & GYNECOL MED ONCOL, HOUSTON, TX 77030 USA. UNIV TEXAS, MD ANDERSON CANC CTR, DEPT BIOMATH, HOUSTON, TX 77030 USA.
authors.forename: M
authors.initials: M
authors.lastname: Cervantes
authors.forename: H
authors.initials: H
authors.lastname: Zhou
authors.forename: A
authors.initials: A
authors.lastname: Sahin
authors.forename: K
authors.initials: K
authors.lastname: Dhingra
authors.forename: V
authors.initials: V
authors.lastname: Valero
authors.forename: N
authors.initials: N
authors.lastname: Ordonez
authors.forename: A
authors.initials: A
authors.lastname: Elnaggar
authors.forename: K
authors.initials: K
authors.lastname: Hess
authors.forename: A
authors.initials: A
authors.lastname: Glassman
authors.forename: S
authors.initials: S
authors.lastname: Sen