title: Detection of glutathione in whole blood after stabilization with N-ethylmaleimide.
abstract: The measurement of glutathione is a challenging task in that, during sample manipulation, a large percentage of this compound can be artificially oxidized. Here a high-performance liquid chromatography (HPLC) method to measure reduced glutathione in blood, based on the analysis of its conjugate with N-ethylmaleimide, has been developed and validated. The method is linear in the concentration range 0.1-2 mM, and the lower limit of quantification is 0.05 mM. Blood samples treated with N-ethylmaleimide are stable at -20°C for 90 days. This method has simplicity and rapidity as its main advantages; therefore, it is particularly suitable for large-scale clinical studies.
authors.affiliation: Department of Evolutionary Biology, Laboratory of Pharmacology and Toxicology, University of Siena, 53100 Siena, Italy. giustarini@unisi.it
authors.forename: Daniela
authors.initials: D
authors.lastname: Giustarini
authors.forename: Isabella
authors.initials: I
authors.lastname: Dalle-Donne
authors.forename: Aldo
authors.initials: A
authors.lastname: Milzani
authors.forename: Ranieri
authors.initials: R
authors.lastname: Rossi
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005033:Ethylmaleimide / Q000737:chemistry*; D005978:Glutathione / Q000097:blood* / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection
chemical_list: D005978:Glutathione; D005033:Ethylmaleimide