title: TP53INP1 overexpression in prostate cancer correlates with poor prognostic factors and is predictive of biological cancer relapse.
abstract: BACKGROUND
  Tumor protein 53-induced nuclear protein 1 (TP53INP1) is a proapoptotic protein involved in cell stress response. Whereas there is an overexpression of TP53INP1 in numerous tissues submitted to stress agents, TP53INP1 is down-expressed in stomach, pancreatic, and inflammation-mediated colic carcinomas. In medullary thyroid carcinomas, TP53INP1 overexpression correlates with poor prognosis. TP53INP1 expression has never been reported in Prostate Cancer (PC). Our aim was to investigate variations of TP53INP1 expression and their correlation to clinicopathological parameters in PC.
  
  
  METHODS
  Quantitative measurements of immunohistochemical expression of TP53INP1 using high-throughput densitometry, assessed on digitized microscopic tissue micro-array images, were correlated with clinicopathological parameters in 91 human PC. Treatment of LNCaP tumor cells in vitro with cytokines and with TP53INP1 antisense oligonucleotide (ASO) was also analyzed.
  
  
  RESULTS
  In normal prostate tissues, TP53INP1 is only expressed in prostate basal cells. There is a de novo TP53INP1 expression in prostate luminal cells in inflammatory prostate tissues, high grade PIN lesions and in PC. Stimulation of LNCaP cells with inflammatory cytokines enhances the level of TP53INP1 mRNA. In PC, TP53INP1 overexpression correlates with high Gleason grade, unfavorable D'Amico score and lymph node invasion, and is an independent factor of biological cancer relapse. Moreover, treatment of LNCaP cells with a TP53INP1 ASO down-regulates TP53INP1 protein level, inhibits proliferation, and induces apoptosis.
  
  
  CONCLUSIONS
  TP53INP1 overexpression in PC seems to be a worse prognostic factor, particularly predictive of biological cancer relapse. Results in vitro suggest that TP53INP1 could be considered as a relevant target for potential specific therapy.
authors.affiliation: Department of Pathology, Hôpital Nord, Chemin des Bourrellys, Marseille, France. sophie.giusiano@ap-hm.fr
authors.forename: Sophie
authors.initials: S
authors.lastname: Giusiano
authors.forename: Stéphane
authors.initials: S
authors.lastname: Garcia
authors.forename: Claudia
authors.initials: C
authors.lastname: Andrieu
authors.forename: Nelson Javier
authors.initials: NJ
authors.lastname: Dusetti
authors.forename: Cyrille
authors.initials: C
authors.lastname: Bastide
authors.forename: Martin
authors.initials: M
authors.lastname: Gleave
authors.forename: Colette
authors.initials: C
authors.lastname: Taranger-Charpin
authors.forename: Juan Lucio
authors.initials: JL
authors.lastname: Iovanna
authors.forename: Palma
authors.initials: P
authors.lastname: Rocchi
mesh_terms: D014408:Biomarkers, Tumor / Q000096:biosynthesis / Q000235:genetics; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics; D005434:Flow Cytometry; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D009364:Neoplasm Recurrence, Local / Q000235:genetics / Q000378:metabolism*; D009376:Neoplasms, Hormone-Dependent / Q000235:genetics / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000235:genetics / Q000378:metabolism*; D012334:RNA, Neoplasm / Q000737:chemistry / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D012044:Regression Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D046888:Tissue Array Analysis
chemical_list: D014408:Biomarkers, Tumor; D002352:Carrier Proteins; D006360:Heat-Shock Proteins; D012334:RNA, Neoplasm; C429767:TP53INP1 protein, human