title: PNAS4 knockout does not induce obviously neurocytes apoptosis and abnormal development in mice brain.
abstract: Apoptosis is one kind of programmed cell death and contributes to development of a variety of organs such as brain. PNAS4 has been reported to be a novel apoptosis-related gene. Overexpression and knocking down of PNAS4 would cause zebrafish and Xenopus lavis developmental abnormalities. But its function and apoptotic mechanism in mammals are still unknown. Here, we first reported that established PNAS4 CKO (conditional knock out) mice using recombineering technology. We prepared its polyclonal antibodies which recognized both myc-PNAS4 overexpression protein and WT and CKO mice brain tissue and MEFS cells with high titre and specificity. Further we detected that PNAS4 was highly expressed in the embryonic period. However, we observed neither neural structural abnormality nor apoptosis signal in PNAS4 CKO mice brain. Our data suggested that PNAS4 was not involved in mice brain development and apoptosis.
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu, 610041, China.
authors.forename: Peiling
authors.initials: P
authors.lastname: Cai
authors.forename: Xiaoqiang
authors.initials: X
authors.lastname: Xia
authors.forename: Fanghua
authors.initials: F
authors.lastname: Li
authors.forename: Yiyuan
authors.initials: Y
authors.lastname: Cui
authors.forename: Qiaona
authors.initials: Q
authors.lastname: Yang
authors.forename: Mina
authors.initials: M
authors.lastname: Chen
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D051017:Apoptosis Regulatory Proteins / Q000235:genetics* / Q000378:metabolism; D015153:Blotting, Western; D001921:Brain / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D057809:HEK293 Cells; D006652:Histological Techniques; D006801:Humans; D020287:In Situ Nick-End Labeling; D051379:Mice; D018345:Mice, Knockout
chemical_list: D051017:Apoptosis Regulatory Proteins; D017931:DNA Primers; C541601:PNAS4 protein, mouse