title: Cardiomyocyte marker expression in a human lymphocyte cell line using mouse cardiomyocyte extract.
abstract: Cell transplantation shows potential for the treatment of cardiac diseases. Embryonic stem cells, cord blood and mesenchymal stem cells have been suggested as sources for transplantation therapy. Because of some technical limitations with the use of stem cells, transdifferentiation of fully differentiated cells is a potentially useful alternative. We investigated whether human peripheral blood cells could transdifferentiate into cardiomyocyte. Transdifferentiation was induced in a human B lymphocyte cell line (Raji). Cardiomyocyte extract was prepared from adult mouse cardiomyocytes. The cells were treated with 5-aza-2-deoxycytidine and trichostatin A, permeabilized with streptolysin O, and exposed to the mouse cardiomyocyte extract. They were cultured for 10 days, 3 weeks and 4 weeks. Cardiomyocyte markers were detected with immunohistochemistry and flow cytometry. Immunocytochemistry revealed that some cells expressed myosin heavy chain, α-actinin and cardiac troponin T after 3 and 4 weeks. Flow cytometry confirmed these data. In cells exposed to trichostatin A and 5-aza-2-deoxycytidine and permeabilized in the presence of the cardiomyocyte extract, troponin T expression was seen in 3.53% of the cells and 3.11% of them expressed α-actinin. After exposure to the cardiomyocyte extract, some permeabilized cells adhered to the plate loosely; however, the morphology did not change significantly, and they continued to show a rounded shape after 4 weeks. Our treated lymphocytes expressed cardiomyocyte markers. Our results suggest that lymphocytes may be useful in future research as a source of cells for reprogramming procedures.
authors.affiliation: Laboratory for Stem Cell Research, Anatomy Department, Shiraz University of Medical Sciences (SUMS), Shiraz, Iran. Vojdaniz@sums.ac.ir
authors.forename: Zahra
authors.initials: Z
authors.lastname: Vojdani
authors.forename: Sima
authors.initials: S
authors.lastname: Tavakolinejad
authors.forename: Tahereh
authors.initials: T
authors.lastname: Talaei-Khozani
authors.forename: Tahereh
authors.initials: T
authors.lastname: Esmaeilpour
authors.forename: Manuchehr
authors.initials: M
authors.lastname: Rasooli
mesh_terms: D000199:Actins / Q000032:analysis; D000818:Animals; D001374:Azacitidine / Q000031:analogs & derivatives / Q000494:pharmacology; D001402:B-Lymphocytes / Q000737:chemistry* / Q000166:cytology; D015415:Biomarkers / Q000032:analysis; D002457:Cell Extracts / Q000494:pharmacology; D002463:Cell Membrane Permeability; D054338:Cell Transdifferentiation; D000077209:Decitabine; D005434:Flow Cytometry; D006801:Humans; D006877:Hydroxamic Acids / Q000494:pharmacology; D007150:Immunohistochemistry; D051379:Mice; D032383:Myocytes, Cardiac / Q000737:chemistry*; D018995:Myosin Heavy Chains / Q000032:analysis; D020107:Troponin T / Q000032:analysis
chemical_list: D000199:Actins; D015415:Biomarkers; D002457:Cell Extracts; D006877:Hydroxamic Acids; D020107:Troponin T; C012589:trichostatin A; D000077209:Decitabine; D018995:Myosin Heavy Chains; D001374:Azacitidine