title: Monoclonal antibodies against lipopolysaccharide of Chlamydia trachomatis with cross reactivity to human ApoB.
abstract: Splenocytes obtained from mice immunized with whole purified elementary bodies of Chlamydia trachomatis were used for hybridoma construction. The resulting clones were screened with ELISA using chlamydial lipopolysaccharide (LPS) and full-length human apolipoprotein B (ApoB). One analyzed clone producing IgG1 (MAb 7B5) showed simultaneous recognition of chlamydial LPS and human ApoB, suggesting the presence of common antigenic epitopes in their structures. MAb 7B5 exhibited agreeable activity in immunoblot analysis conducted using chlamydial extracts or full-length human ApoB as well as in immunofluorescence (IF) detecting typical inclusion bodies of C. trachomatis and C. pneumoniae in the infected eukaryotic host cells. The removal of LPS from chlamydial suspensions with lauroyl sarcosyl led to a complete disappearance of IF associated with the elementary bodies of C. trachomatis. Therefore, immunologic response to chlamydial antigen may be associated with the generation of ApoB-specific antibody. Molecular mimicry and subsequent formation of cross-reactive antibodies might be an essential mechanism explaining the appearance of circulating auto-antibodies against low density lipoproteins (LDL) in patients with atherosclerosis. Moreover, newly generated MAb 7B5 can be a useful tool in the laboratory diagnosis of chlamydial infections.
authors.affiliation: Cambridge Theranostics Ltd., Babraham Research Campus, Babraham, Cambridge, United Kingdom.
authors.forename: Ivan M
authors.initials: IM
authors.lastname: Petyaev
authors.forename: Nayilia A
authors.initials: NA
authors.lastname: Zigangirova
authors.forename: Valeriy V
authors.initials: VV
authors.lastname: Tsibezov
authors.forename: Agnij
authors.initials: A
authors.lastname: Ross
authors.forename: Yuriy K
authors.initials: YK
authors.lastname: Bashmakov
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000276:immunology* / Q000302:isolation & purification; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology* / Q000302:isolation & purification; D000942:Antigens, Bacterial / Q000032:analysis / Q000276:immunology; D001055:Apolipoproteins B / Q000276:immunology*; D050197:Atherosclerosis / Q000276:immunology; D015153:Blotting, Western; D002690:Chlamydia Infections / Q000175:diagnosis / Q000276:immunology*; D002692:Chlamydia trachomatis / Q000737:chemistry / Q000276:immunology*; D003429:Cross Reactions / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006825:Hybridomas / Q000276:immunology / Q000378:metabolism; D007074:Immunoglobulin G / Q000276:immunology* / Q000302:isolation & purification; D008070:Lipopolysaccharides / Q000276:immunology*; D051379:Mice; D018716:Molecular Mimicry / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001055:Apolipoproteins B; D007074:Immunoglobulin G; D008070:Lipopolysaccharides