title: Passive immunization with Leptospira LPS-specific agglutinating but not non-agglutinating monoclonal antibodies protect guinea pigs from fatal pulmonary hemorrhages induced by serovar Copenhageni challenge.
abstract: Leptospira interrogans serovar Copenhageni causes pulmonary hemorrhages with respiratory failure, a major cause of death in leptospirosis patients. Protective immunity to Leptospira is known to correlate with the production of leptospiral lipopolysaccharide (L-LPS)-specific agglutinating antibodies. We generated L-LPS-specific mouse monoclonal antibodies (MAbs) and investigated if these MAbs can protect guinea pigs against fatal pulmonary hemorrhages caused by serovar Copenhageni. The MAbs L8H4 and L9B11 against 22kDa L-LPS agglutinated leptospires and completely protected guinea pigs from the development of fatal pulmonary hemorrhages by serovar Copenhageni, whereas the MAb L4C1 against 8kDa L-LPS neither agglutinated the bacteria nor protected the animals against the fatal pulmonary hemorrhages.
authors.affiliation: Department of Biomedical Sciences, Tufts University Cummings School of Veterinary Medicine, 200 Westboro Road, North Grafton, MA 01536, USA.
authors.forename: Sreerupa
authors.initials: S
authors.lastname: Challa
authors.forename: Jarlath E
authors.initials: JE
authors.lastname: Nally
authors.forename: Carroll
authors.initials: C
authors.lastname: Jones
authors.forename: Abhineet S
authors.initials: AS
authors.lastname: Sheoran
mesh_terms: D000371:Agglutination / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage* / Q000276:immunology; D000918:Antibody Specificity; D004195:Disease Models, Animal; D006168:Guinea Pigs; D006470:Hemorrhage / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D006801:Humans; D007116:Immunization, Passive; D015151:Immunoblotting; D007921:Leptospira interrogans / Q000145:classification / Q000276:immunology* / Q000472:pathogenicity; D007922:Leptospirosis / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D008070:Lipopolysaccharides / Q000276:immunology*; D008171:Lung Diseases / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D008297:Male; D051379:Mice; D012703:Serotyping; D014895:Weil Disease
chemical_list: D000911:Antibodies, Monoclonal; D008070:Lipopolysaccharides