title: Chromatin under mechanical stress: from single 30 nm fibers to single nucleosomes.
abstract: About a decade ago, the elastic properties of a single chromatin fiber and, subsequently, those of a single nucleosome started to be explored using optical and magnetic tweezers. These techniques have allowed direct measurements of several essential physical parameters of individual nucleosomes and nucleosomal arrays, including the forces responsible for the maintenance of the structure of both the chromatin fiber and the individual nucleosomes, as well as the mechanism of their unwinding under mechanical stress. Experiments on the assembly of individual chromatin fibers have illustrated the complexity of the process and the key role of certain specific components. Nevertheless a substantial disparity exists in the data reported from various experiments. Chromatin, unlike naked DNA, is a system which is extremely sensitive to environmental conditions, and studies carried out under even slightly different conditions are difficult to compare directly. In this review we summarize the available data and their impact on our knowledge of both nucleosomal structure and the dynamics of nucleosome and chromatin fiber assembly and organization.
authors.affiliation: CNRS, Laboratoire de Spectrometrie Physique, St Martin d'Heres, France. jbedn@lf1.cuni.cz
authors.forename: Jan
authors.initials: J
authors.lastname: Bednar
authors.forename: Stefan
authors.initials: S
authors.lastname: Dimitrov
mesh_terms: D000818:Animals; D002843:Chromatin / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D009707:Nucleosomes / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
chemical_list: D002843:Chromatin; D009707:Nucleosomes