title: Identification of the strong vasorelaxing substance scirpusin B, a dimer of piceatannol, from passion fruit (Passiflora edulis) seeds.
abstract: Piceatannol is present in passion fruit (Passiflora edulis) seeds in high amounts. In this study, we isolated the second major polyphenolic compound of passion fruit seeds and identified it as scirpusin B, which is a dimer of piceatannol. We investigated the antioxidant activities and vasorelaxing effects of these polyphenols. Their antioxidant effects were measured using an in vitro 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay, and their vasorelaxant effects were determined ex vivo in rat thoracic aorta. Both polyphenolic compounds exhibited potent antioxidant activities and significant vasorelaxant effects in endothelium-intact aortas. More specifically, scirpusin B exerted a greater antioxidant activity and vasorelaxant effect compared with that of piceatannol. Additionally, the vasorelaxation effects of the compounds were induced via the NO derived from the endothelium. This study provides the possibility that polyphenols in passion fruit seeds are effective against cardiovascular diseases (CVDs).
authors.affiliation: Health Care Division, Morinaga and Company, Limited , 2-1-1 Shimosueyoshi, Tsurumi-ku, Yokohama 230-8504, Japan. s-sano-jh@morinaga.co.jp
authors.forename: Shoko
authors.initials: S
authors.lastname: Sano
authors.forename: Kenkichi
authors.initials: K
authors.lastname: Sugiyama
authors.forename: Tatsuhiko
authors.initials: T
authors.lastname: Ito
authors.forename: Yumi
authors.initials: Y
authors.lastname: Katano
authors.forename: Akira
authors.initials: A
authors.lastname: Ishihata
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification; D001011:Aorta / Q000187:drug effects / Q000502:physiology; D001572:Benzofurans / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D019281:Dimerization; D005638:Fruit / Q000737:chemistry; D066298:In Vitro Techniques; D008297:Male; D015394:Molecular Structure; D029598:Passiflora / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D051381:Rats; D011916:Rats, Inbred F344; D012639:Seeds / Q000737:chemistry*; D013267:Stilbenes / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D014665:Vasodilator Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D001572:Benzofurans; D010936:Plant Extracts; D013267:Stilbenes; D014665:Vasodilator Agents; C546905:scirpusin B