title: Changes in chromosomes 1 and 6 associated with human cell immortalisation.
abstract: A normal diploid human fibroblast cell line infected with HL60 c-myc has become immortal in vitro following continuous passage. At early passage post-infection the cells exhibited a normal diploid karyotype and in the absence of karyotypic changes grew well beyond the normal passage at which senescence occurred in the normal non-transfected parental cell line or the vector-infected control. Immortalisation of both the infected population and a subclone taken prior to karyotypic change was associated with two independent events involving chromosome 1. The subcloned population also possessed a deletion of the long arm of chromosome 6.
authors.affiliation: UNIV LIVERPOOL, DEPT SURG, CELLULAR ONCOL GRP, LIVERPOOL L69 3GA, MERSEYSIDE, ENGLAND. CHRISTIE HOSP & HOLT RADIUM INST, PATERSON INST CANC RES, CRC, DEPT CANC GENET, MANCHESTER M20 9BX, LANCS, ENGLAND.
authors.forename: E
authors.initials: E
authors.lastname: Mitchell
authors.forename: A
authors.initials: A
authors.lastname: Kinsella