title: Sucrose monolaurate synthesis with Protex 6L immobilized on electrospun TiO2 nanofiber.
abstract: TiO(2) nanofibers with uniform diameter about 125 nm were prepared based on sol-gel process and electrospinning technology. Protex 6L, an industrial alkaline protease, was covalently immobilized on TiO(2) nanofiber through γ-aminopropyltriethoxysilane modification and glutaraldehyde crosslinking. With 2 (v/v)% glutaraldehyde as crosslinker, the enzyme loading is about 201 mg (g nanofiber membrane)(-1), and the specific activity of the immobilized Protex 6L is 2.45 μmol h(-1) ml(-1) mg(-1) protein for synthesis of sucrose monolaurate from sucrose and vinyl laurate. The optimal condition for sucrose monolaurate production is 5% (v/v) water content in DMSO/2-methyl-2-butanol solvent mixture and 50 °C. Under this condition, 97% conversion was achieved within 36 h by nanofibrous Protex 6L, which is corresponding to a productivity 34 times higher than that of most widely used Novozym 435. After 10 cycles reuse, nanofibrous Protex 6L retained 52.4% of its original activity.
authors.affiliation: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Xin-Ran
authors.initials: XR
authors.lastname: Wang
authors.forename: Song-Ping
authors.initials: SP
authors.lastname: Zhang
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D010450:Endopeptidases / Q000378:metabolism*; D004800:Enzymes, Immobilized / Q000378:metabolism*; D053768:Metal Nanoparticles; D057139:Nanofibers; D011485:Protein Binding; D013395:Sucrose / Q000031:analogs & derivatives* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D004800:Enzymes, Immobilized; C018152:sucrose monolaurate; D013395:Sucrose; D010450:Endopeptidases; C087822:alkaline protease