title: Breast-carcinoma cell-lines with metastatic potential in mice.
abstract: We established and characterized three human breast carcinoma cell lines (two with metastatic potential) by in vivo-in vitro propagation of tumor tissues. Tissues were processed in culture as i) direct explants (n = 185); ii) cell suspensions obtained after enzymic digestion (n = 29); or iii) explants of xenografts established in vivo (n = 18). Tissues processed as explants or cell suspensions generated no viable cell lines. However, three cell lines (MAXF-401, MAXF-583 and OHSTMAM-4) were established from xenografts. All three showed characteristic features of human breast tumor tissue. All were tumorigenic in mice and MAXF-401 and MAXF-583 showed frequent distant metastasis following s.c. transplantation. All three overexpressed EGFR, p53 and c-erbB2 proteins.
authors.affiliation: COOK CTY HOSP,CHICAGO,IL 60612. HEKTOEN INST MED RES,CHICAGO,IL 60612.
authors.forename: R
authors.initials: R
authors.lastname: Mehta
authors.forename: J
authors.initials: J
authors.lastname: Graves
authors.forename: A
authors.initials: A
authors.lastname: Shilkaitis
authors.forename: G
authors.initials: G
authors.lastname: Hart
authors.forename: T
authors.initials: T
authors.lastname: Dasgupta