title: [Comparative study on the osteogenic differentiation of rat bone marrow stromal cells effected by icariin and icariside II].
abstract: OBJECTIVE
  To investigate the effects of icariin and it's main metabolites-icariside II on the osteogenic differentiation of rat bone marrow stromal cells (rBMSCs).
  
  
  METHODS
  rBMSCs were cultured by adherence screening method, icariin and icariside II were supplemented into the culture at 5 x 10(-5) mol/L respectively. The osteogenic differentiation markers including alkaline phosphatase (ALP) activity, CFU-F(ALp), osteocalcin secretion, calcium deposition and mineralized bone modulus were compared among the icariin-supplemented group, icariside II and the control. The gene expressions of bFGF, IGF-1, Osterix and Runx-2 were examined by RT-Real Time PCR.
  
  
  RESULTS
  Both icariside II and icariin significantly improved ALP activity, CFU-F(ALP) amount, osteocalcin secretion, calcium deposition and mineralized modulus. Besides, they enhanced the gene expressions of bFGF, IGF-1, Osterix and Runx-2. Icariside II was obviously stronger than icariin at the above activities.
  
  
  CONCLUSIONS
  Icariside II is stronger than icariin at enhancing the osteogenic differentiation of rBMSCs, suggesting that icariin can be administered via oral and it's metabolites are the effective constitutes for antiosteoporosis activity.
authors.affiliation: Institute of Orthopaedics, Lanzhou General Hospital, Lanzhou 730050, China. zhaiyk1984@163.com
authors.forename: Yuan-Kun
authors.initials: YK
authors.lastname: Zhai
authors.forename: Bao-Feng
authors.initials: BF
authors.lastname: Ge
authors.forename: Ke-Ming
authors.initials: KM
authors.lastname: Chen
authors.forename: Hui-Ping
authors.initials: HP
authors.lastname: Ma
authors.forename: Lei-Guo
authors.initials: LG
authors.lastname: Ming
authors.forename: Zhi-Feng
authors.initials: ZF
authors.lastname: Li
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000235:genetics / Q000378:metabolism; D005419:Flavonoids / Q000494:pharmacology*; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000378:metabolism; D015675:Osteocalcin / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017154:Stromal Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D012333:RNA, Messenger; D016222:Fibroblast Growth Factor 2; D015675:Osteocalcin; C060988:baohuoside I; D007334:Insulin-Like Growth Factor I; D000469:Alkaline Phosphatase; C056599:icariin