title: Flavonoids from Daphne aurantiaca and their inhibitory activities against nitric oxide production.
abstract: Chemical examination of the methanolic extract from the stem bark of Daphne aurantiaca led to the isolation of three new flavonoids (1-3), and 29 known flavonoids. All 32 compounds were isolated for the first time from Daphne aurantiaca. The isolates were tested for inhibitory activities against lipopolysaccharide (LPS)-induced nitric oxide (NO) production in RAW 264.7 macrophages. Compounds 21 and 24 showed potent inhibitory activities against the production of NO with IC₅₀ values of 0.006 and 0.076 µM, respectively.
authors.affiliation: School of Pharmacy, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Liang
authors.forename: Jun-Mian
authors.initials: JM
authors.lastname: Tian
authors.forename: Yi
authors.initials: Y
authors.lastname: Feng
authors.forename: Xiao-Hua
authors.initials: XH
authors.lastname: Liu
authors.forename: Zhi
authors.initials: Z
authors.lastname: Xiong
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Zhang
mesh_terms: D032364:Daphne / Q000737:chemistry* / Q000378:metabolism; D005419:Flavonoids / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification* / Q000494:pharmacology*; D020128:Inhibitory Concentration 50; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D000432:Methanol / Q000737:chemistry; D009569:Nitric Oxide / Q000096:biosynthesis*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors*; D010936:Plant Extracts / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification / Q000494:pharmacology; D013056:Spectrophotometry, Ultraviolet
chemical_list: D005419:Flavonoids; D008070:Lipopolysaccharides; D010936:Plant Extracts; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D000432:Methanol