title: Cytotoxicity of a plant steroidal saponin on human lung cancer cells.
abstract: Trillium tschonoskii Maxim has been used to treat several diseases, including cancers, in folk medicine. The primary objective of the present study was to test whether TTB2, a steroidal saponin isolated from n-BuOH extracts, might exert effects on cell survival, morphology, cell cycle, reactive oxygen species (ROS) production and mitochondrial function in a lung cancer cell line. It was found that TTB2 had anti-proliferative and morphological influence, leading to the loss of mitochondrial membrane potential (MMP) in a dose-dependent manner. A significant increase in the level of intracellular ROS and an accumulation of cells in the G2/M phases of the cell cycle were also observed in treated cells. In summary, our results indicated that this compound might have potential use for the treatment of cancer.
authors.affiliation: Hubei Key Laboratory of Natural Products Research and Development, Medical College, China Three Gorges University, Yichang, China. kzou@sina.cn
authors.forename: Wenfeng
authors.initials: W
authors.lastname: Huang
authors.forename: Kun
authors.initials: K
authors.lastname: Zou
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects*; D008928:Mitochondria / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012503:Saponins / Q000494:pharmacology*; D031446:Trillium / Q000737:chemistry; D014407:Tumor Cells, Cultured
chemical_list: D010936:Plant Extracts; D017382:Reactive Oxygen Species; D012503:Saponins