title: Directed differentiation of motor neuron cell-like cells from human adipose-derived stem cells in vitro.
abstract: The capacity of human adipose-derived stem cells (hADSCs) to differentiate into motor neurons and the identity of molecular factors that confer hADSCs with the competence of motor neurons have yet to be elucidated. Here, retinoic acid and sonic hedgehog were applied to examine whether hADSCs could be differentiated into motor neurons. As early as 6 h after induction, hADSCs were changed toward neuronal morphology. After induction, hADSCs showed positive immunocytochemical staining for β-III-tubulin, choline acetyltransferase, and neuron-specific enolase. Reverse-transcriptase polymerase chain reaction characterization indicated that cells differentiated from hADSCs were restricted to the ventral spinal fate (Nkx2.2, Pax6, Hb9, and Olig2). Our results suggest that hADSCs may be a potential candidate in cellular therapy for motor neuron disease.
authors.affiliation: Department of Neurology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liqing
authors.forename: Geng
authors.initials: G
authors.lastname: Jia
authors.forename: Cao
authors.initials: C
authors.lastname: Jiqing
authors.forename: Gu
authors.initials: G
authors.lastname: Ran
authors.forename: Chen
authors.initials: C
authors.lastname: Fei
authors.forename: Kong
authors.initials: K
authors.lastname: Jie
authors.forename: Wang
authors.initials: W
authors.lastname: Yanyun
authors.forename: Zhang
authors.initials: Z
authors.lastname: Cheng
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D053687:Adult Stem Cells / Q000166:cytology / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D000090322:Homeobox Protein Nkx-2.2; D018398:Homeodomain Proteins; D006801:Humans; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology; D009046:Motor Neurons / Q000166:cytology* / Q000187:drug effects; D058953:Neural Stem Cells / Q000166:cytology* / Q000187:drug effects; D009687:Nuclear Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors
chemical_list: D000090322:Homeobox Protein Nkx-2.2; D018398:Homeodomain Proteins; D036341:Intercellular Signaling Peptides and Proteins; C000715631:NKX2-2 protein, human; D009687:Nuclear Proteins; D014157:Transcription Factors