title: Effects of atrazine and chlorpyrifos on the mRNA levels of IL-1 and IFN-γ2b in immune organs of common carp.
abstract: Atrazine (ATR) and chlorpyrifos (CPF) are widely used in agriculture has resulted in a series of toxicological and environmental problems. The aim of this study was to investigate the effects of ATR, CPF and their mixture on the mRNA levels of interleukin-1β (IL-1β), interleukin receptor I (IL-1RI) and interferon gamma (IFN-γ2b) in both spleen and head kidney of Common carp. In this study, juvenile common carp were exposed to ATR (at concentrations of 4.28, 42.8 and 428 μg/L), CPF (at concentrations of 1.16, 11.6 and 116 μg/L), and their mixture (at concentrations of 1.16, 11.6 and 116 μg/L). The mRNA levels of IL-1β, IL-1R1 and IFN-γ2b in spleen and head kidney were detected by using RT-PCR. Our results indicated that IL-1β, IL-1R1 expression significantly increased after exposure in high concentration ATR, CPF and their mixture, but IFN-γ2b mRNA shown different expression trends. Our results suggested that ATR, CPF and their mixture probably induced damages on spleen and head kidney may be association with increasing IL-1β, IL-1R1 mRNA synthesis. After 20-day recovery test, IL-1β, IL-1R1 and IFN-γ2b mRNA expression remain at high level in majority of the treated groups, we concluded that the restoration of tissue and immune system damage probably needs longer time.
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Harbin 150030, PR China.
authors.forename: Xu
authors.initials: X
authors.lastname: Wang
authors.forename: Houjuan
authors.initials: H
authors.lastname: Xing
authors.forename: Xuelin
authors.initials: X
authors.lastname: Li
authors.forename: Shiwen
authors.initials: S
authors.lastname: Xu
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D001280:Atrazine / Q000276:immunology* / Q000633:toxicity; D002347:Carps / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D004390:Chlorpyrifos / Q000276:immunology* / Q000633:toxicity; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D007371:Interferon-gamma / Q000235:genetics* / Q000378:metabolism; D053583:Interleukin-1beta / Q000235:genetics* / Q000378:metabolism; D007668:Kidney / Q000276:immunology; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D053573:Receptors, Interleukin-1 Type I / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013154:Spleen / Q000276:immunology
chemical_list: D029941:Fish Proteins; D053583:Interleukin-1beta; D012333:RNA, Messenger; D053573:Receptors, Interleukin-1 Type I; D007371:Interferon-gamma; D004390:Chlorpyrifos; D001280:Atrazine