title: Arabidopsis CPR5 independently regulates seed germination and postgermination arrest of development through LOX pathway and ABA signaling.
abstract: The phytohormone abscisic acid (ABA) and the lipoxygenases (LOXs) pathway play important roles in seed germination and seedling growth and development. Here, we reported on the functional characterization of Arabidopsis CPR5 in the ABA signaling and LOX pathways. The cpr5 mutant was hypersensitive to ABA in the seed germination, cotyledon greening and root growth, whereas transgenic plants overexpressing CPR5 were insensitive. Genetic analysis demonstrated that CPR5 gene may be located downstream of the ABI1 in the ABA signaling pathway. However, the cpr5 mutant showed an ABA independent drought-resistant phenotype. It was also found that the cpr5 mutant was hypersensitive to NDGA and NDGA treatment aggravated the ABA-induced delay in the seed germination and cotyledon greening. Taken together, these results suggest that the CPR5 plays a regulatory role in the regulation of seed germination and early seedling growth through ABA and LOX pathways independently.
authors.affiliation: Guangdong Key Lab of Biotechnology for Plant Development, College of Life Science, South China Normal University, Guangzhou, China.
authors.forename: Guilan
authors.initials: G
authors.lastname: Gao
authors.forename: Shengchun
authors.initials: S
authors.lastname: Zhang
authors.forename: Chengfeng
authors.initials: C
authors.lastname: Wang
authors.forename: Xiang
authors.initials: X
authors.lastname: Yang
authors.forename: Yaqin
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Su
authors.forename: Jinju
authors.initials: J
authors.lastname: Du
authors.forename: Chengwei
authors.initials: C
authors.lastname: Yang
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism*; D017360:Arabidopsis / Q000196:embryology / Q000235:genetics / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000502:physiology*; D018506:Gene Expression Regulation, Plant; D018525:Germination; D058945:Lipoxygenases / Q000378:metabolism*; D008565:Membrane Proteins / Q000502:physiology*; D018613:Microscopy, Confocal; D016133:Polymerase Chain Reaction; D012639:Seeds / Q000502:physiology*; D015398:Signal Transduction
chemical_list: D029681:Arabidopsis Proteins; C528008:CPR5 protein, Arabidopsis; D008565:Membrane Proteins; D000040:Abscisic Acid; D058945:Lipoxygenases