title: [Study of Streptococcus pneumoniae strains resistant to antibiotics analyzed at "Cantacuzino" NIRDMI during 2006-2008].
abstract: OBJECTIVE
  The aim of the this study was the analysis of the resistance to antibiotics of Streptococcus pneumoniae isolated in last years.
  
  
  METHODS
  328 S. pneumoniae strains, coming from blood, CSF tracheal aspirate (TA), or sputum, pleural fluid (PL) and other samples (ear and sinus fluid) isolated in 2006-2008, were analyzed at INCDMI "Cantacuzino", National Reference Center for Streptococcus pneumoniae. Strains were tested for susceptibility to by agar diution method (minimal inhibitory concentration-MIC) to the following antibiotics: penicillin (Pc), erythromycin (Em), cephalothin (Kf). cefuroxim (Cxm), cefotaxim (Ctx), trimethoprim/sulfamethoxazol (Sxt), ofloxacin (Ojx), amoxicillin (Amx). tetracycline (Te), cloramphenicol (Cm), vancomycin (Va).
  
  
  RESULTS
  The analysis of the results was done according to CLSI 2009. Pneumococci strains isolated from blood, CSF, TA or sputum and PL showed lower resistance level to antibiotics (38.8% Pc, 9.3% Cxm. 4.1% Ctx, 2.7% Amx. 24% Em, 2.4% Ofx, 68% Sxt) against those isolated from ear ans sinus fluid which revealed high levels of resistance (70% Pc, 11.2 % Cxm, 5.9 % Ctx, 3.4% Amx, 58.4 % Em. 3.8% Ofx, 73% Sxt). Strains resistant to penicillin, isolated from blood and CSF revealed the following aspects: 17% low level of resistance and 11 % high level of resistance. CONCLUSIONS. The most efficient antibiotics were Ctx, Amx and Oft. A continuous surveillance of pneumococci strains resistant to antibiotics is needed, as well as the use of an pneumococcal efficient vaccine.
authors.affiliation: INCDMI Cantacuzino Bucureşti.
authors.forename: Marina
authors.initials: M
authors.lastname: Pană
authors.forename: Maria
authors.initials: M
authors.lastname: Ghiţă
authors.forename: Maria
authors.initials: M
authors.lastname: Nica
authors.forename: Smaranda
authors.initials: S
authors.lastname: Botea
authors.forename: Olga-Mihaela
authors.initials: OM
authors.lastname: Dorobăţ
authors.forename: Alexandru
authors.initials: A
authors.lastname: Rafila
authors.forename: Raluca
authors.initials: R
authors.lastname: Papagheorghe
authors.forename: Nicoleta
authors.initials: N
authors.lastname: Popescu
authors.forename: Irina
authors.initials: I
authors.lastname: Nistor
authors.forename: Vasilica
authors.initials: V
authors.lastname: Ungureanu
mesh_terms: D000047:Academies and Institutes; D000658:Amoxicillin / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000627:therapeutic use; D035843:Biomedical Research; D002439:Cefotaxime / Q000494:pharmacology; D002444:Cefuroxime / Q000494:pharmacology; D002512:Cephalothin / Q000494:pharmacology; D002701:Chloramphenicol / Q000494:pharmacology; D004917:Erythromycin / Q000494:pharmacology; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D015242:Ofloxacin / Q000494:pharmacology; D010406:Penicillins / Q000494:pharmacology; D012189:Retrospective Studies; D012383:Romania; D013296:Streptococcus pneumoniae / Q000145:classification / Q000187:drug effects / Q000302:isolation & purification*; D013752:Tetracycline / Q000494:pharmacology; D015662:Trimethoprim, Sulfamethoxazole Drug Combination / Q000494:pharmacology; D014640:Vancomycin / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D010406:Penicillins; D004917:Erythromycin; D002701:Chloramphenicol; D014640:Vancomycin; D000658:Amoxicillin; D015662:Trimethoprim, Sulfamethoxazole Drug Combination; D015242:Ofloxacin; D013752:Tetracycline; D002439:Cefotaxime; D002444:Cefuroxime; D002512:Cephalothin