title: Transgenic production of arachidonic acid in oilseeds.
abstract: We describe a transgenic microalgal Δ9-elongase pathway transformed in both Brassica napus and Arabidopsis thaliana seed resulting in the production of arachidonic acid (ARA). This pathway is noteworthy for both the production of ARA in seed tissue and the low levels of intermediate C20 fatty acids that accumulate. We also demonstrate that the arachidonic acid is naturally enriched at the sn2 position in triacylglycerol. This is the first report of ARA production by the Δ9-elongase pathway in an oilseed.
authors.affiliation: Food Futures National Research Flagship, CSIRO Plant Industry, PO Box 1600, Canberra, ACT, 2601, Australia.
authors.forename: James R
authors.initials: JR
authors.lastname: Petrie
authors.forename: Pushkar
authors.initials: P
authors.lastname: Shrestha
authors.forename: Srinivas
authors.initials: S
authors.lastname: Belide
authors.forename: Maged P
authors.initials: MP
authors.lastname: Mansour
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.forename: James
authors.initials: J
authors.lastname: Horne
authors.forename: Peter D
authors.initials: PD
authors.lastname: Nichols
authors.forename: Surinder P
authors.initials: SP
authors.lastname: Singh
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D016718:Arachidonic Acid / Q000096:biosynthesis* / Q000378:metabolism; D029688:Brassica napus / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D004798:Enzymes / Q000235:genetics / Q000378:metabolism; D005822:Genetic Vectors; D030821:Plants, Genetically Modified / Q000378:metabolism; D012639:Seeds / Q000235:genetics / Q000378:metabolism*; D014280:Triglycerides / Q000737:chemistry
chemical_list: D004798:Enzymes; D014280:Triglycerides; D016718:Arachidonic Acid