title: Environmental stress-dependent effects of deletions encompassing Hsp70Ba on canalization and quantitative trait asymmetry in Drosophila melanogaster.
abstract: Hsp70 genes may influence the expression of wing abnormalities in Drosophila melanogaster but their effects on variability in quantitative characters and developmental instability are unclear. In this study, we focused on one of the six Hsp70 genes, Hsp70Ba, and investigated its effects on within- and among-individual variability in orbital bristle number, sternopleural bristle number, wing size and wing shape under different environmental conditions. To do this, we studied a newly constructed deletion, Df(3R)ED5579, which encompasses Hsp70Ba and nine non-Hsp genes, in the heterozygous condition and another, Hsp70Ba(304), which deletes only Hsp70Ba, in the homozygous condition. We found no significant effect of both deletions on within-individual variation quantified by fluctuating asymmetry (FA) of morphological traits. On the other hand, the Hsp70Ba(304)/Hsp70Ba(304) genotype significantly increased among-individual variation quantified by coefficient of variation (CV) of bristle number and wing size in female, while the Df(3R)ED5579 heterozygote showed no significant effect. The expression level of Hsp70Ba in the deletion heterozygote was 6 to 20 times higher than in control homozygotes, suggesting that the overexpression of Hsp70Ba did not influence developmental stability or canalization significantly. These findings suggest that the absence of expression of Hsp70Ba increases CV of some morphological traits and that HSP70Ba may buffer against environmental perturbations on some quantitative traits.
authors.affiliation: Department of Population Genetics, National Institute of Genetics, Mishima, Shizuoka-ken, Japan. kaz_tak@cc.okayama-u.ac.jp
authors.forename: Kazuo H
authors.initials: KH
authors.lastname: Takahashi
authors.forename: Phillip J
authors.initials: PJ
authors.lastname: Daborn
authors.forename: Ary A
authors.initials: AA
authors.lastname: Hoffmann
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Takano-Shimizu
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism; D004331:Drosophila melanogaster / Q000235:genetics* / Q000254:growth & development*; D004777:Environment; D005260:Female; D017353:Gene Deletion; D018507:Gene Expression Regulation, Developmental; D005838:Genotype; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D018869:Heat-Shock Response / Q000235:genetics; D007814:Larva / Q000235:genetics; D019655:Quantitative Trait, Heritable; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013312:Stress, Physiological / Q000235:genetics*; D013696:Temperature; D014921:Wings, Animal / Q000033:anatomy & histology / Q000254:growth & development / Q000378:metabolism
chemical_list: D029721:Drosophila Proteins; D018840:HSP70 Heat-Shock Proteins; C560434:Hsp70Ba protein, Drosophila; D012333:RNA, Messenger