title: Development of a lab-on-a-chip device for diagnosis of plant pathogens.
abstract: A lab-on-a-chip system for rapid nucleic acid-based analysis was developed that can be applied for diagnosis of selected Phytophthora species as a first example for use in plant pathology. All necessary polymerase chain reaction process (PCR) and hybridization steps can be performed consecutively within a single chip consisting of two components, an inflexible and a flexible one, with integrated microchannels and microchambers. Data from the microarray is collected from a simple electrical measurement that is based on elementary silver deposition by enzymatical catalyzation. Temperatures in the PCR and in the hybridization zone are managed by two independent Peltier elements. The chip will be integrated in a compact portable system with a pump and power supply for use on site. The specificity of the lab-on-a-chip system could be demonstrated for the tested five Phytophthora species. The two Pythium species gave signals below the threshold. The results of the electrical detection of the microarray correspond to the values obtained with the control method (optical grey scale analysis).
authors.affiliation: Institute of Photonic Technology (IPHT), Nanobiophotonics Department, Albert-Einstein-Str. 9, 07745 Jena, Germany. sandra.julich@ipht-jena.de
authors.forename: Sandra
authors.initials: S
authors.lastname: Julich
authors.forename: Marko
authors.initials: M
authors.lastname: Riedel
authors.forename: Mark
authors.initials: M
authors.lastname: Kielpinski
authors.forename: Matthias
authors.initials: M
authors.lastname: Urban
authors.forename: Robert
authors.initials: R
authors.lastname: Kretschmer
authors.forename: Stefan
authors.initials: S
authors.lastname: Wagner
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Fritzsche
authors.forename: Thomas
authors.initials: T
authors.lastname: Henkel
authors.forename: Robert
authors.initials: R
authors.lastname: Möller
authors.forename: Sabine
authors.initials: S
authors.lastname: Werres
mesh_terms: D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004867:Equipment Design; D056656:Lab-On-A-Chip Devices; D010838:Phytophthora / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D010935:Plant Diseases / Q000469:parasitology; D010944:Plants / Q000469:parasitology*; D016133:Polymerase Chain Reaction; D013045:Species Specificity
chemical_list: D017931:DNA Primers