title: [HPLC-UV-ELSD characteristic figure and chemical pattern recognition of Panacis Quinquefolii Radix].
abstract: The paper is to report the establishment of a method of characteristic figure analysis for the quality control of Panacis Quinquefolii Radix. Application of HPLC-UV-ELSD techniques was connected in series and applied. The separation was carried out on the Agilent Extend-C18 (250 mm x 4.6 mm, 5 microm) column. The mobile phase consisted of water and acetonitrile with gradient elution. The flow rate was 1.0 mL x min(-1) and the wavelength of measurement was 203 nm. The temperature of drift tube was maintained at 106.5 degrees C and the flow rate of air was set at 2.9 L x min(-1). Twenty batches of the Panacis Quinquefolii Radix were determined. Hierarchical cluster analysis (HCA) and principal component analysis (PCA) were applied to study on the HPLC characteristic figure and chemical pattern recognition. The HPLC-UV and HPLC-ELSD characteristic figure of Panacis Quinquefolii Radix was developed, the ginsenosides Rg1, Re, Rb1, Rc, Rb2, Rb3, Rd and the pseudoginsenoside F11 were identified. This method is accurate and reliable, and it can be used to control the quality of the Panacis Quinquefolii Radix.
authors.affiliation: Henan University of Traditional Chinese Medicine, Zhengzhou 450003, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Dong
authors.forename: Cui-ying
authors.initials: CY
authors.lastname: Zhang
authors.forename: Shi-lin
authors.initials: SL
authors.lastname: Chen
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D016000:Cluster Analysis; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D036145:Ginsenosides / Q000032:analysis*; D005894:Panax / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D025341:Principal Component Analysis; D011786:Quality Control
chemical_list: D004365:Drugs, Chinese Herbal; D036145:Ginsenosides; C413985:pseudoginsenoside F11; C035054:ginsenoside Rg1