title: In vitro screening of ovarian tumor specific peptides from a phage display peptide library.
abstract: To develop more biomarkers for diagnosis and therapy of ovarian cancer, a 12-mer phage display library was used to isolate peptides that bound specifically to the human ovarian tumor cell line SK-OV-3. After five rounds of in vitro screening, the recovery rate of phages showed a 69-fold increase over the first round of washings and a group of phage clones capable of binding to SK-OV-3 cells were obtained. A phage clone named Z1 with high affinity and specificity to SK-OV-3 cells was identified in vitro. More importantly, the synthetic biotin-labeled peptide, ZP1 (=SVSVGMKPSPRP), which corresponded to the sequence of the inserted fragment of Z1, demonstrated a high specificity to SK-OV-3 cells especially when compared to other cell lines (A2780 and 3T3). ZP1 might therefore be a biomarker for targeting drug delivery in ovarian cancer therapy.
authors.affiliation: College of Materials Science and Engineering, Sichuan University, No. 24, South 1st Section, 1st Ring Road, Chengdu, Sichuan 610065, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Guangfu
authors.initials: G
authors.lastname: Yin
authors.forename: Danhong
authors.initials: D
authors.lastname: Yan
authors.forename: Yan
authors.initials: Y
authors.lastname: Wei
authors.forename: Chuying
authors.initials: C
authors.lastname: Ma
authors.forename: Zhongbing
authors.initials: Z
authors.lastname: Huang
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Liao
authors.forename: Yadong
authors.initials: Y
authors.lastname: Yao
authors.forename: Xianchun
authors.initials: X
authors.lastname: Chen
authors.forename: Baoqing
authors.initials: B
authors.lastname: Hao
mesh_terms: D000951:Antigens, Neoplasm / Q000032:analysis*; D014408:Biomarkers, Tumor / Q000032:analysis; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D008403:Mass Screening; D010051:Ovarian Neoplasms / Q000175:diagnosis*; D019151:Peptide Library; D010455:Peptides / Q000302:isolation & purification*; D011485:Protein Binding; D012680:Sensitivity and Specificity
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D019151:Peptide Library; D010455:Peptides