title: Development of a new selective medium for isolation of methicillin-resistant Staphylococcus aureus.
abstract: A new selective medium containing cephem antibiotics was developed for isolation of methicillin-resistant Staphylococcus aureus (MRSA). MRSA colonies on a medium containing ceftazidime (CAZ) were most easily identifiable and a medium containing cefoperazone (CPZ) was superior in suppressing the growth of other bacteria. With the medium containing a couple of CAZ and CPZ, MRSA and methicillin-resistant coagulase-negative staphylococci (MRCNS) were detected from 2 and 1 of 15 chicken meat samples respectively. The MRSA and MRCNS recovery test showed that the medium was effective for MRSA isolation, suppressing the growth of other bacteria efficiently. These results suggested that the medium containing a couple of CAZ and CPZ was useful for MRSA detection from foods and animals.
authors.affiliation: Department of Bioresource and Agrobiosciences, Graduate School of Agricultural, Kobe University, 1–1 Rokkodai-cho, Nada-ku, Kobe, Hyogo 657–8501, Japan. Etsuko_Saitou01@pref.hyogo.lg.jp
authors.forename: Etsuko
authors.initials: E
authors.lastname: Saito
authors.forename: Yoko
authors.initials: Y
authors.lastname: Kakita
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Shimoda
authors.forename: Shizuka
authors.initials: S
authors.lastname: Shimabukuro
authors.forename: Keiichi
authors.initials: K
authors.lastname: Saeki
authors.forename: Akira
authors.initials: A
authors.lastname: Shimizu
authors.forename: Junichi
authors.initials: J
authors.lastname: Kawano
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001431:Bacteriological Techniques; D002645:Chickens; D003470:Culture Media / Q000737:chemistry*; D005516:Food Microbiology; D008460:Meat / Q000382:microbiology; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects / Q000502:physiology*
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media