title: Clinical study of cultured epithelial autografts in liquid suspension in severe burn patients.
abstract: We address the clinical application of the suspension type cultured epithelial autografts (CEAs), Keraheal™ (MCTT, Seoul, Korea), along with the effects, application method, merits and demerits thereof. From February 2007 to June 2010, 29 burn patients with extensive burns, participated in the suspension type of CEA clinical test. A widely meshed autograft (1:4-6 ratio) was applied to the wound bed and the suspension type CEA was sprayed with a Tissomat cell sprayer, followed by a Tissucol spray, a fibrin sealant. The patients' (men/women=26/3) median (interquartile ranges) age was 42 (30-49) years old, the burned TBSA was 55 (44-60) %, and the full thickness burn area was 40 (30-46.5) %. The area of Keraheal™ applied was 800 (400-1200) cm(2). The take rate was 96 (90.5-99) % and 100 (98.5-100) % at 2 and 4 weeks after treatment with Keraheal™, respectively. The Vancouver burn scar scale was 5 (4-6.5), 4 (3-6), and 3 (2-4) at 8, 12 and 24 weeks after the Keraheal™ application. Widely meshed autograft must be applied in massive burns but it's take rate is greatly reduced. The CEAs enhance the take rate of a wide meshed autograft in massive burns and allow for grafting wide meshed autograft together with acellular dermal matrix in some cases.
authors.affiliation: Department of Burn Surgery, Hangang Sacred Heart Hospital, Hallym University Medical Center, Youngdeungpo-gu, Seoul, Republic of Korea.
authors.forename: Haejun
authors.initials: H
authors.lastname: Yim
authors.forename: Hyeong Tae
authors.initials: HT
authors.lastname: Yang
authors.forename: Yong Suk
authors.initials: YS
authors.lastname: Cho
authors.forename: Cheong Hoon
authors.initials: CH
authors.lastname: Seo
authors.forename: Boung Chul
authors.initials: BC
authors.lastname: Lee
authors.forename: Jang Hyu
authors.initials: JH
authors.lastname: Ko
authors.forename: In Suk
authors.initials: IS
authors.lastname: Kwak
authors.forename: Dohern
authors.initials: D
authors.lastname: Kim
authors.forename: Jun
authors.initials: J
authors.lastname: Hur
authors.forename: Jong Hyun
authors.initials: JH
authors.lastname: Kim
authors.forename: Wook
authors.initials: W
authors.lastname: Chun
mesh_terms: D000328:Adult; D002056:Burns / Q000473:pathology / Q000601:surgery*; D004847:Epithelial Cells / Q000637:transplantation*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology; D008297:Male; D008875:Middle Aged; D009330:Nebulizers and Vaporizers; D011446:Prospective Studies; D046509:Tissue Culture Techniques / Q000379:methods*; D014182:Transplantation, Autologous