title: Hypo- and hyperthyroidism affect NEI concentration in discrete brain areas of adult male rats.
abstract: To date, there has been only one in vitro study of the relationship between neuropeptide EI (NEI) and the hypothalamic-pituitary-thyroid (HPT) axis. To investigate the possible relationship between NEI and the HPT axis, we developed a rat model of hypothyroidism and hyperthyroidism that allows us to determine whether NEI content is altered in selected brain areas after treatment, as well as whether such alterations are related to the time of day. Hypothyroidism and hyperthyroidism, induced in male rats, with 6-propyl-1-thiouracil and l-thyroxine, respectively, were confirmed by determination of triiodothyronine, total thyroxine, and thyrotropin levels. All groups were studied at the morning and the afternoon. In rats with hypothyroidism, NEI concentration, evaluated on postinduction days 7 and 24, was unchanged or slightly elevated on day 7 but was decreased on day 24. In rats with hyperthyroidism, NEI content, which was evaluated after 4 days of l-thyroxine administration, was slightly elevated, principally in the preoptic area in the morning and in the median eminence-arcuate nucleus and pineal gland in the afternoon, the morning and afternoon NEI contents being similar in the controls. These results provide the bases to pursue the study of the interaction between NEI and the HPT axis.
authors.affiliation: Laboratorio de Ciencias Fisiológicas, Cátedra de Bacteriología y Virología Médicas, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, CP 5000 Córdoba, Argentina.
authors.forename: Carolina
authors.initials: C
authors.lastname: Ayala
authors.forename: Susana Ruth
authors.initials: SR
authors.lastname: Valdez
authors.forename: María Luján Navarra
authors.initials: ML
authors.lastname: Morero
authors.forename: Marta
authors.initials: M
authors.lastname: Soaje
authors.forename: Norma Beatriz
authors.initials: NB
authors.lastname: Carreño
authors.forename: Mónica Silvina
authors.initials: MS
authors.lastname: Sanchez
authors.forename: Jakson Cioni
authors.initials: JC
authors.lastname: Bittencourt
authors.forename: Graciela Alma
authors.initials: GA
authors.lastname: Jahn
authors.forename: María Ester
authors.initials: ME
authors.lastname: Celis
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D006980:Hyperthyroidism / Q000139:chemically induced / Q000378:metabolism* / Q000503:physiopathology; D007037:Hypothyroidism / Q000139:chemically induced / Q000378:metabolism* / Q000503:physiopathology; D008297:Male; D008473:Median Eminence / Q000187:drug effects / Q000378:metabolism*; D009842:Oligopeptides / Q000096:biosynthesis*; D010902:Pituitary Gland / Q000187:drug effects / Q000378:metabolism*; D011441:Propylthiouracil / Q000009:adverse effects; D012333:RNA, Messenger; D051381:Rats; D017207:Rats, Sprague-Dawley; D013961:Thyroid Gland / Q000187:drug effects / Q000378:metabolism*; D013972:Thyrotropin / Q000096:biosynthesis; D013974:Thyroxine / Q000009:adverse effects; D014284:Triiodothyronine / Q000096:biosynthesis
chemical_list: D009842:Oligopeptides; D012333:RNA, Messenger; C522850:neuropeptide EI; D014284:Triiodothyronine; D011441:Propylthiouracil; D013972:Thyrotropin; D013974:Thyroxine