title: Proteomic analysis of inviable salmonid hybrids between female masu salmon Oncorhynchus masou masou and male rainbow trout Oncorhynchus mykiss during early embryogenesis.
abstract: Early embryos of inviable hybrids between female masu salmon Oncorhynchus masou masou and male rainbow trout Oncorhynchus mykiss at 9, 12, 15 and 20 days after fertilization were examined for protein expression profiles. A total of 44 proteins, mostly down-regulated products of house-keeping genes and those involved in nucleic acid metabolism or chromatin replication, were identified in hybrid embryos by mass spectrometry analysis and protein database searching. The identified down-regulated proteins may be responsible for the inviability in the hybrids.
authors.affiliation: Laboratory of Aquaculture Genetics and Genomics, Graduate School of Fisheries Sciences, Hokkaido University, 3-1-1 Minato, Hakodate 041-8611, Japan. dong589@hotmail.com
authors.forename: L
authors.initials: L
authors.lastname: Zheng
authors.forename: H
authors.initials: H
authors.lastname: Tanaka
authors.forename: S
authors.initials: S
authors.lastname: Abe
mesh_terms: D000818:Animals; D004625:Embryo, Nonmammalian / Q000378:metabolism*; D047108:Embryonic Development / Q000502:physiology; D005260:Female; D029941:Fish Proteins / Q000737:chemistry / Q000378:metabolism*; D006824:Hybridization, Genetic; D008297:Male; D013058:Mass Spectrometry; D017683:Oncorhynchus / Q000196:embryology* / Q000378:metabolism / Q000502:physiology; D017686:Oncorhynchus mykiss / Q000196:embryology* / Q000378:metabolism / Q000502:physiology; D020543:Proteome; D040901:Proteomics
chemical_list: D029941:Fish Proteins; D020543:Proteome