title: Ribosome-dependent ATPase interacts with conserved membrane protein in Escherichia coli to modulate protein synthesis and oxidative phosphorylation.
abstract: Elongation factor RbbA is required for ATP-dependent deacyl-tRNA release presumably after each peptide bond formation; however, there is no information about the cellular role. Proteomic analysis in Escherichia coli revealed that RbbA reciprocally co-purified with a conserved inner membrane protein of unknown function, YhjD. Both proteins are also physically associated with the 30S ribosome and with members of the lipopolysaccharide transport machinery. Genome-wide genetic screens of rbbA and yhjD deletion mutants revealed aggravating genetic interactions with mutants deficient in the electron transport chain. Cells lacking both rbbA and yhjD exhibited reduced cell division, respiration and global protein synthesis as well as increased sensitivity to antibiotics targeting the ETC and the accuracy of protein synthesis. Our results suggest that RbbA appears to function together with YhjD as part of a regulatory network that impacts bacterial oxidative phosphorylation and translation efficiency.
authors.affiliation: Banting and Best Department of Medical Research, University of Toronto, Toronto, Ontario, Canada.
authors.forename: Mohan
authors.initials: M
authors.lastname: Babu
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Aoki
authors.forename: Wasimul Q
authors.initials: WQ
authors.lastname: Chowdhury
authors.forename: Alla
authors.initials: A
authors.lastname: Gagarinova
authors.forename: Chris
authors.initials: C
authors.lastname: Graham
authors.forename: Sadhna
authors.initials: S
authors.lastname: Phanse
authors.forename: Ben
authors.initials: B
authors.lastname: Laliberte
authors.forename: Noor
authors.initials: N
authors.lastname: Sunba
authors.forename: Matthew
authors.initials: M
authors.lastname: Jessulat
authors.forename: Ashkan
authors.initials: A
authors.lastname: Golshani
authors.forename: Andrew
authors.initials: A
authors.lastname: Emili
authors.forename: Jack F
authors.initials: JF
authors.lastname: Greenblatt
authors.forename: M Clelia
authors.initials: MC
authors.lastname: Ganoza
mesh_terms: D000251:Adenosine Triphosphatases / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D002455:Cell Division; D004579:Electron Transport; D029968:Escherichia coli Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D047468:Immunoprecipitation; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D010085:Oxidative Phosphorylation; D014176:Protein Biosynthesis; D012270:Ribosomes / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; D008565:Membrane Proteins; C528319:YhjD protein, E coli; D000251:Adenosine Triphosphatases; C422450:RbbA protein, E coli