title: Effects of antisense oligonucleotides targeting k-ras expression in pancreatic-cancer cell-lines.
abstract: The K-ras oncogene is activated in more than 75% of pancreatic cancers and represents a compelling target for therapeutic intervention. We have examined the potential of K-ras antisense oligonucleotides in human pancreatic cancer cell lines. Dose-dependent antiproliferative effects are seen, but these do not correlate with reduced K-ras levels in the treated cells. Although patterns of uptake can be altered by treatment with cationic lipids, this does not enhance antiproliferative effects nor reduce K-ras expression. The antisense approach requires considerable development before useful anti-cancer agents can emerge and alternative approaches are needed for K-ras to be a target for genetic intervention therapy for human cancer.
authors.affiliation: HAMMERSMITH HOSP,ROYAL POSTGRAD MED SCH,ICRF ONCOL UNIT,MOLEC PATHOL LAB,LONDON W12 0NN,ENGLAND. IMPERIAL CANC RES FUND,VIDEOMICROSCOPY & MICROINJECT LAB,LONDON WC2A 3PX,ENGLAND.
authors.forename: G
authors.initials: G
authors.lastname: Carter
authors.forename: C
authors.initials: C
authors.lastname: Gilbert
authors.forename: N
authors.initials: N
authors.lastname: Lemoine