title: [Study of a novel compound 2460A with activities produced by fungus].
abstract: With IL-6R as target, a new compound 2460A was identified from fungus using HTS screening model. The taxonomics of the produced strain was confirmed to be Trichoderma hazianum rifai after sequencing analysis of rDNA-ITS (internal transcribed spacer). Results showed that this compound has a binding activity on IL-6R competed with IL-6, thus it is a new ligand of IL-6R originating from microbe. With MTT assay, the anti-tumor activities of 2460A were demonstrated on CM126 and HT-29 cell lines separately, the IC50 are 2.17 x 10(-5) mol x L(-1) and 1.8 x 10(-5) mol x L(-1) respectively. The compound affected lightly the HT-29 cell cycle at S phase. Studies for the anti-tumor activity of 2460A in vivo are in progress in our lab.
authors.affiliation: Ministry of Health Key Laboratory of Biotechnology of Antibiotics, Institute of Medicinal Biotechnology Chinese Academy of Medical Sciences & Peking Union Medical College, Bejing 100050, China.
authors.forename: Xiao-qiang
authors.initials: XQ
authors.lastname: Qi
authors.forename: Feng-chang
authors.initials: FC
authors.lastname: Zhu
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.forename: Lian-hong
authors.initials: LH
authors.lastname: Guo
authors.forename: Rong
authors.initials: R
authors.lastname: Jiang
authors.forename: Qi-yang
authors.initials: QY
authors.lastname: He
authors.forename: Yuan
authors.initials: Y
authors.lastname: Li
mesh_terms: D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D001667:Binding, Competitive; D019046:Bone Marrow Neoplasms / Q000473:pathology*; D002453:Cell Cycle / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D019073:HT29 Cells; D057166:High-Throughput Screening Assays; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D008024:Ligands; D019947:Receptors, Interleukin-6 / Q000378:metabolism; D014242:Trichoderma / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D015850:Interleukin-6; D008024:Ligands; D019947:Receptors, Interleukin-6