title: Rapid quantification of four major bioactive alkaloids in Corydalis decumbens (Thunb.) Pers. by pressurised liquid extraction combined with liquid chromatography-triple quadrupole linear ion trap mass spectrometry.
abstract: A new method based on pressurised liquid extraction (PLE) followed by liquid chromatography-triple quadrupole linear ion trap mass spectrometry (LC-QTrap-MS) analysis has been developed for the identification and quantification of four major alkaloids in extracts of Corydalis decumbens (Thunb.) Pers. PLE extractions were performed using 90% ethanol; temperature was set at 100°C and pressure at 1500 psi. HPLC analysis was performed on a Waters XBridge™ C(18) column (150 mm × 2.1mm i.d., 3.5 μm) eluted by a mobile phase of acetonitrile and 0.2% acetic acid. Data acquisition was carried out in multiple reaction monitoring transitions (MRMs) mode, monitoring two MRM transitions to ensure an accurate identification of target compounds in the samples. Additional identification and confirmation of target compounds were performed using the enhanced product ion modus (EPI) of the linear ion trap. The novel LC-QTrap-MS platform offers the best sensitivity and specificity for characterization and quantitative determination of the four alkaloids in C. decumbens (Thunb.) Pers. and fulfils the quality criteria for routine laboratory application.
authors.affiliation: College of Chemistry and Materials Engineering, Wenzhou University, Wenzhou 325035, China. lwsheny@yahoo.com.cn
authors.forename: Yan
authors.initials: Y
authors.lastname: Shen
authors.forename: Chao
authors.initials: C
authors.lastname: Han
authors.forename: Yongxiang
authors.initials: Y
authors.lastname: Jiang
authors.forename: Xiujin
authors.initials: X
authors.lastname: Zhou
authors.forename: Zhenou
authors.initials: Z
authors.lastname: Zhu
authors.forename: Xinxiang
authors.initials: X
authors.lastname: Lei
mesh_terms: D000470:Alkaloids / Q000032:analysis* / Q000302:isolation & purification* / Q000378:metabolism; D005591:Chemical Fractionation / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D031311:Corydalis / Q000737:chemistry*; D057230:Limit of Detection; D016014:Linear Models; D013058:Mass Spectrometry / Q000379:methods*; D010936:Plant Extracts / Q000737:chemistry; D011312:Pressure; D015203:Reproducibility of Results; D013010:Sonication; D053719:Tandem Mass Spectrometry; D013997:Time Factors
chemical_list: D000470:Alkaloids; D010936:Plant Extracts