title: The effect of interleukin-10 gene promoter polymorphisms on early-onset coronary artery disease.
abstract: OBJECTIVE
  We assessed the association between interleukin-10 (IL-10) -1082G/A and -592C/A polymorphisms, and coronary heart disease (CHD).
  
  
  METHODS
  A cross-sectional, observational study included 86 patients (mean age 43.36±4.930 years) diagnosed to have CHD and 88 healthy controls (mean age 47.07±8.135 years). IL-10 -1082G/A and -592C/A polymorphisms were analyzed using restriction fragment length polymorphism (RFLP) and agarose gel electrophoresis methods in both patient and control groups. Genotype distributions of the polymorphisms between CHD patients and controls were assessed by Chi-square analysis.
  
  
  RESULTS
  The genotype distribution of the -1082 G/A polymorphism was not different in premature CHD patients (GG: 38.3%; GA: 51.1%; AA: 10.6%) and controls (GG: 43.1%; GC: 43.1%; CC: 13.8%) (p=0.57). The prevalence of the A allele at -1082G/A polymorphism was 36.6% in patients and 35.3% in controls. Both allele and genotype frequencies of -592C/A polymorphism did not also differ significantly between patients with CHD and controls. We did not observe relationships between polymorphism-specific haplotypes and adverse angiographic and clinical outcomes. We have observed a significant difference of IL-10 -592C/A allelic frequency (OR=2.00 95% CI=0.9434-4.2579) between the younger CHD patients (<45 years, Group 2) and matched controls.
  
  
  CONCLUSIONS
  Our study suggests that IL-10-592C/A polymorphism may play a role in susceptibility to CHD in younger patients.
authors.affiliation: Department of Medical Genetics, Faculty of Medicine, Ege University, Izmir, Turkey. emin.karaca@ege.edu.tr
authors.forename: Emin
authors.initials: E
authors.lastname: Karaca
authors.forename: Meral
authors.initials: M
authors.lastname: Kayıkçıoğlu
authors.forename: Hüseyin
authors.initials: H
authors.lastname: Onay
authors.forename: Cumhur
authors.initials: C
authors.lastname: Gündüz
authors.forename: Ferda
authors.initials: F
authors.lastname: Ozkınay
mesh_terms: D000328:Adult; D000367:Age Factors; D016022:Case-Control Studies; D003327:Coronary Disease / Q000097:blood / Q000235:genetics*; D003430:Cross-Sectional Studies; D004587:Electrophoresis, Agar Gel; D005260:Female; D005838:Genotype; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011401:Promoter Regions, Genetic / Q000235:genetics
chemical_list: D016753:Interleukin-10