title: Genotyping of potentially zoonotic Giardia duodenalis from exotic and wild animals kept in captivity in Brazil.
abstract: We have studied the variability of glutamate dehydrogenase (gdh) and small subunit ribosomal (SSU) rRNA coding genes of Giardia species in fecal samples isolated from wild and exotic animals in Brazil, and compared with homologous sequences of isolates from human and domestic animals characterized in previous studies. Cysts of Giardia duodenalis were obtained from feces of naturally infected monkeys (Alouatta fusca) (n=20), chinchillas (Chinchilla lanigera) (n=3), ostriches (Struthio camelus) (n=2) and jaguar (Panthera onca) (n=1). Assemblage AI was assigned to the unique isolate of jaguar. All the samples from monkeys, chinchillas, and ostriches were assigned to Assemblage B. There was little evolutionary divergence between the referred isolates and isolates described elsewhere. The Assemblage B isolates identified in this study were closely related to Assemblage BIV isolated from humans. The molecular identification of Assemblages A and B of G. duodenalis isolates from exotic and wild animals demonstrates that such hosts may be a potential reservoir for zoonotic transmission of G. duodenalis.
authors.affiliation: Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, CEP 05508-270 São Paulo, SP, Brazil. rosoares@usp.br
authors.forename: Rodrigo Martins
authors.initials: RM
authors.lastname: Soares
authors.forename: Sílvio Luís Pereira
authors.initials: SL
authors.lastname: de Souza
authors.forename: Luciane Holsback
authors.initials: LH
authors.lastname: Silveira
authors.forename: Mikaela Renata
authors.initials: MR
authors.lastname: Funada
authors.forename: Leonardo José
authors.initials: LJ
authors.lastname: Richtzenhain
authors.forename: Solange M
authors.initials: SM
authors.lastname: Gennari
mesh_terms: D000383:Agriculture; D000818:Animals; D000835:Animals, Wild; D001938:Brazil / Q000453:epidemiology; D005243:Feces / Q000469:parasitology; D005786:Gene Expression Regulation / Q000502:physiology; D014644:Genetic Variation; D005838:Genotype; D016829:Giardia lamblia / Q000235:genetics* / Q000302:isolation & purification*; D005873:Giardiasis / Q000635:transmission / Q000662:veterinary*; D005969:Glutamate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D006801:Humans; D057805:Pets; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000235:genetics / Q000378:metabolism; D015047:Zoonoses / Q000469:parasitology*
chemical_list: D012335:RNA, Ribosomal; D005969:Glutamate Dehydrogenase