title: Discrimination of Alicyclobacillus strains using nitrocellulose membrane filter and attenuated total reflectance fourier transform infrared spectroscopy.
abstract: Alicyclobacillus spp. are thermoacidophilic, spore-forming bacteria, some of which cause spoilage in pasteurized and heat-treated apple juice products through the production of guaiacol. It would be helpful if a rapid method to detect and discriminate Alicyclobacillus strains was available. A simple and rapid sample preparation method using nitrocellulose membrane filter (NMF) and a single reflection horizontal attenuated total reflection (HATR) accessory with Fourier transform infrared (FT-IR) was developed here. Fourier transform infrared (FT-IR) spectroscopy was used and tested on 8 Alicyclobacillus strains (KF, WAC, NWN-13501, NWN-12697, NWN-12654, NWN-10682, 1016, 1101). A linear discriminant analysis (LDA) was established to discriminate Alicyclobacillus strains. The sample preparation method could successfully separated strains into different groups by principal component analysis (PCA). High identification accuracy (95%) was achieved with the LDA model.
  
  
  CONCLUSIONS
  The method developed in the paper can be used to discriminate different Alicyclobacillus strains from each other making it possible to easily determine whether the strain of Alicyclobacillus present is associated with juice spoilage.
authors.affiliation: College of Food Science and Engineering, Northwest A&F Univ, Yangling, Shaanxi, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Tianli
authors.initials: T
authors.lastname: Yue
authors.forename: Yahong
authors.initials: Y
authors.lastname: Yuan
authors.forename: Xiaonan
authors.initials: X
authors.lastname: Lu
authors.forename: Joong-Han
authors.initials: JH
authors.lastname: Shin
authors.forename: Barbara
authors.initials: B
authors.lastname: Rasco
mesh_terms: D056450:Alicyclobacillus / Q000145:classification / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques; D001628:Beverages / Q000382:microbiology; D003101:Collodion / Q000378:metabolism*; D016002:Discriminant Analysis; D005374:Filtration; D005506:Food Contamination / Q000032:analysis*; D016014:Linear Models; D015999:Multivariate Analysis; D025341:Principal Component Analysis; D013045:Species Specificity; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
chemical_list: D003101:Collodion