title: Nanoscale-engineered cytochrome p450 system with a branch structure.
abstract: Most of the bacterial cytochrome P450 s require two kinds of electron transfer proteins, ferredoxin and ferredoxin reductase, and thus P450 s do not show catalytic activity by themselves. A microbial transglutaminase-mediated site-specific cross-linking enables the formation of fusion P450 protein with a branched structure, which is generated from a genetic fusion protein of P450-ferredoxin reductase and ferredoxin, an interactive nanoscale protein structure. This fusion P450 system is self-sufficient due to intramolecular electron transfer, which means the system does not require additional electron-transferring proteins. Because some components of bacterial cytochrome P450 system are interchangeable, this self-sufficient system can be applied to non-natural combination of P450 and electron transfer proteins from different species of bacteria.
authors.affiliation: Department of Bioengineering, Graduate School of Engineering, Center for NanoBio Integration, The University of Tokyo, Tokyo 113-8656, Japan.
authors.forename: Hidehiko
authors.initials: H
authors.lastname: Hirakawa
authors.forename: Teruyuki
authors.initials: T
authors.lastname: Nagamune
mesh_terms: D000595:Amino Acid Sequence; D001419:Bacteria / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism*; D004579:Electron Transport; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli; D005288:Ferredoxins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009247:NADH, NADPH Oxidoreductases / Q000235:genetics / Q000378:metabolism; D036103:Nanotechnology / Q000379:methods; D010084:Oxidation-Reduction; D015202:Protein Engineering / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*; D013057:Spectrum Analysis; D011503:Transglutaminases / Q000235:genetics / Q000378:metabolism*
chemical_list: D005288:Ferredoxins; D011993:Recombinant Fusion Proteins; D003577:Cytochrome P-450 Enzyme System; D009247:NADH, NADPH Oxidoreductases; C022486:putidaredoxin reductase; D011503:Transglutaminases