title: [Stromal cells inhibit Kallikrein 7 expression in the epithelial cells of benign prostate hyperplasia].
abstract: OBJECTIVE
  To investigate the influence of stromal cells on the Kallikrein 7 (KLK7) expression of epithelial cells in benign prostate hyperplasia (BPH).
  
  
  METHODS
  We constructed a stromal-epithelial co-culture model after separating the two types of cells from BPH tissues and identifying them by cell morphology and chemiluminescent microparticle immunoassay (CMIA). The expression of KLK7 mRNA was detected by RT-PCR in the epithelial cells with or without the stromal cells, and that of the KLK7 protein (hK7) determined by Western blot.
  
  
  RESULTS
  Stromal and epithelial cells were successfully separated and identified, and a stromal-epithelial co-culture model successfully established. RT-PCR showed that the mRNA expression of the KLK7 gene was higher in the epithelial cells co-cultured with stromal cells than in the epithelial cells alone, and the gray value of KLK7 to GAPDH was 1.41 +/- 0.041 in the former and 1.78 +/- 0.10 in the latter (P < 0.01). The results of Western blot were consistent with those of RT-PCR.
  
  
  CONCLUSIONS
  Stromal cells can suppress the expression of the KLK7 gene in the epithelial cells in BPH. KLK7 may be involved in the change of epithelial cells stimulated by stromal cells.
authors.affiliation: Medical Scientific Research Center, Guangxi Medical University, Nanning, Guangxi 530021, China. cncsyxl@gmail.com
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Yang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Xuan
authors.forename: Feng-Yu
authors.initials: FY
authors.lastname: Huang
authors.forename: You-Hong
authors.initials: YH
authors.lastname: Pang
authors.forename: Zeng-Nan
authors.initials: ZN
authors.lastname: Mo
mesh_terms: D002478:Cells, Cultured; D006801:Humans; D007610:Kallikreins / Q000378:metabolism*; D008297:Male; D011467:Prostate / Q000378:metabolism*; D011470:Prostatic Hyperplasia / Q000378:metabolism* / Q000473:pathology; D017154:Stromal Cells / Q000378:metabolism*
chemical_list: C082472:KLK7 protein, human; D007610:Kallikreins