title: Assessment of in vivo and in vitro tuberculosis diagnostic tests in Mycobacterium caprae naturally infected caprine flocks.
abstract: Caprine tuberculosis in Spain is mainly caused by Mycobacterium caprae although the progression of the disease and lesion severity is similar to that caused by Mycobacterium bovis. In this study, the sensitivity of the gamma-interferon (IFN-γ) assay using an antigen cocktail containing early secretory antigenic target-6kDa (ESAT-6) and culture filtrate protein 10 (CFP-10) peptides for stimulation was determined and compared with those obtained in single intradermal tuberculin (SIT) and single intradermal cervical comparative tuberculin (SICCT) tests and IFN-γ assay using purified protein derivative (PPD) in three different flocks infected with M. caprae under different epidemiological conditions. Correlation between specific IFN-γ production and severity of lesions was also evaluated. Sensitivities of the diagnostic tests varied greatly in the three flocks studied, with higher values in those where higher lesion scores were observed. The results show that IFN-γ assay applied in goats using PPD or the ESAT-6/CFP-10 peptides cocktail for stimulation yielded similar sensitivity values. A significant yet weak positive correlation between specific IFN-γ production and lesion scores was detected after the stimulation with PPDs (p=0.004) whereas when the blood samples were stimulated with ESAT-6/CFP-10 peptides, the correlation was not significant (p>0.05). Therefore, specific-IFN-γ production after the stimulation with PPDs or ESAT-6/CFP-10 was not an accurate indicator of lesion severity in naturally tuberculosis infected goats with M. caprae.
authors.affiliation: Centro VISAVET, Universidad Complutense de Madrid, 28040 Madrid, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Bezos
authors.forename: Julio
authors.initials: J
authors.lastname: Alvarez
authors.forename: Lucía
authors.initials: L
authors.lastname: de Juan
authors.forename: Beatriz
authors.initials: B
authors.lastname: Romero
authors.forename: Sabrina
authors.initials: S
authors.lastname: Rodríguez
authors.forename: Isabel G
authors.initials: IG
authors.lastname: Fernández-de-Mera
authors.forename: R Glyn
authors.initials: RG
authors.lastname: Hewinson
authors.forename: Martin
authors.initials: M
authors.lastname: Vordermeier
authors.forename: Ana
authors.initials: A
authors.lastname: Mateos
authors.forename: Lucas
authors.initials: L
authors.lastname: Domínguez
authors.forename: Alicia
authors.initials: A
authors.lastname: Aranaz
mesh_terms: D000818:Animals; D015511:Goat Diseases / Q000175:diagnosis* / Q000382:microbiology; D006041:Goats; D007371:Interferon-gamma; D009161:Mycobacterium / Q000276:immunology; D016133:Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000032:analysis; D012680:Sensitivity and Specificity; D013030:Spain; D014373:Tuberculin; D014374:Tuberculin Test / Q000379:methods* / Q000662:veterinary*; D014376:Tuberculosis / Q000175:diagnosis / Q000662:veterinary*
chemical_list: C505464:ESAT-6-CFP10 fusion protein; D011993:Recombinant Fusion Proteins; D014373:Tuberculin; D007371:Interferon-gamma