title: Stability of recombinant bovine interferon-γ antiviral activity in the absence of stabilizing additives.
abstract: The stability of recombinant bovine interferon-γ (rbIFN-γ) produced by a baculovirus expression system was investigated under different storage conditions: freezing-thawing and storage for 30 days at temperatures of -80, 4, 25, and 37°C. Antiviral activity was not significantly decreased by freeze-thawing at least five times. Furthermore, although not statistically different, antiviral activity gradually decreased as temperature increased. These findings suggest that rbIFN-γ possesses high thermal and freeze-thaw stability.
authors.affiliation: Harbin Veterinary Research Institute, 427 Maduan Street, Harbin 150001, China.
authors.forename: Qingyuan
authors.initials: Q
authors.lastname: Xu
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Nomura
authors.forename: Masahiro
authors.initials: M
authors.lastname: Ikeda
authors.forename: Masato
authors.initials: M
authors.lastname: Ohta
authors.forename: Ken-Ichiro
authors.initials: K
authors.lastname: Kameyama
authors.forename: Misako
authors.initials: M
authors.lastname: Konishi
authors.forename: Donglai
authors.initials: D
authors.lastname: Wu
authors.forename: Shigeki
authors.initials: S
authors.lastname: Inumaru
authors.forename: Kenji
authors.initials: K
authors.lastname: Murakami
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D016367:Baculoviridae / Q000235:genetics; D002417:Cattle; D004355:Drug Stability; D004926:Escherichia coli / Q000235:genetics; D007371:Interferon-gamma / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000494:pharmacology; D013696:Temperature; D013997:Time Factors
chemical_list: D000998:Antiviral Agents; D011994:Recombinant Proteins; D007371:Interferon-gamma