title: Analysis of properties and proinflammatory functions of cockroach allergens Per a 1.01s.
abstract: Cockroaches have been identified as one of the major indoor allergens inducing perennial rhinitis and asthma. Per a 1s are a group of the major allergens from American cockroach. Although Per a 1s are major allergens from American cockroach, factors contributing to the allergenicity of Per a 1s are still poorly defined. To investigate the effects of Per a 1s on the expression of PARs and the release of proinflammatory cytokines from mast cells. Per a 1.0101 and Per a 1.0104 were cloned from American cockroach and then expressed in Eschericia coli. The purified allergens were used to stimulate P815 mast cells, and the expression of protease-activated receptors (PARs) was determined by real-time RT-PCR and flow cytometry. The levels of IL-4 and IL-13 in culture media were detected with ELISA. Sera from 80 and 77.3% of cockroach allergy patients reacted to recombinant Per a (rPer a) 1.0101 and rPer a 1.0104, confirming they are major allergens. Both rPer a 1.0101 and rPer a 1.0104 had no enzymatic activity, but rPer a 1.0101 upregulated the expression of PAR-1 and PAR-2, and rPer a 1.0104 enhanced the expression of PAR-1 and PAR-4 proteins. Both recombinant allergens were able to increase the release of IL-4 and IL-13 from P815 mast cells. This is the first study aiming to investigate functions of group 1 allergens of American cockroach. rPer a 1.0101 and rPer a 1.0104 have the capacity to upregulate the expression of PARs and to enhance Th2 cytokine production in mast cells.
authors.affiliation: Clinical Research Centre, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, ChinaThe Key Immunopharmacology Laboratory of Guangdong Province, Allergy and Inflammation Research Institute, Shantou University Medical College, Shantou 515041, ChinaDepartment of Pathophysiology, Hainan Medical College, Haikou, Hainan 571101, ChinaSchool of Life Sciences, Huaiyin Normal University, Huaiyin, Jiangsu, 223300Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, Canada.
authors.forename: S
authors.initials: S
authors.lastname: He
authors.affiliation: Clinical Research Centre, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, ChinaThe Key Immunopharmacology Laboratory of Guangdong Province, Allergy and Inflammation Research Institute, Shantou University Medical College, Shantou 515041, ChinaDepartment of Pathophysiology, Hainan Medical College, Haikou, Hainan 571101, ChinaSchool of Life Sciences, Huaiyin Normal University, Huaiyin, Jiangsu, 223300Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, Canada.
authors.forename: Z
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Clinical Research Centre, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, ChinaThe Key Immunopharmacology Laboratory of Guangdong Province, Allergy and Inflammation Research Institute, Shantou University Medical College, Shantou 515041, ChinaDepartment of Pathophysiology, Hainan Medical College, Haikou, Hainan 571101, ChinaSchool of Life Sciences, Huaiyin Normal University, Huaiyin, Jiangsu, 223300Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, Canada.
authors.forename: H
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Clinical Research Centre, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, ChinaThe Key Immunopharmacology Laboratory of Guangdong Province, Allergy and Inflammation Research Institute, Shantou University Medical College, Shantou 515041, ChinaDepartment of Pathophysiology, Hainan Medical College, Haikou, Hainan 571101, ChinaSchool of Life Sciences, Huaiyin Normal University, Huaiyin, Jiangsu, 223300Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, Canada.
authors.forename: J
authors.initials: J
authors.lastname: Wei
authors.affiliation: Clinical Research Centre, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, ChinaThe Key Immunopharmacology Laboratory of Guangdong Province, Allergy and Inflammation Research Institute, Shantou University Medical College, Shantou 515041, ChinaDepartment of Pathophysiology, Hainan Medical College, Haikou, Hainan 571101, ChinaSchool of Life Sciences, Huaiyin Normal University, Huaiyin, Jiangsu, 223300Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, Canada.
authors.forename: L
authors.initials: L
authors.lastname: Yang
authors.affiliation: Clinical Research Centre, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, ChinaThe Key Immunopharmacology Laboratory of Guangdong Province, Allergy and Inflammation Research Institute, Shantou University Medical College, Shantou 515041, ChinaDepartment of Pathophysiology, Hainan Medical College, Haikou, Hainan 571101, ChinaSchool of Life Sciences, Huaiyin Normal University, Huaiyin, Jiangsu, 223300Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, Canada.
authors.forename: H
authors.initials: H
authors.lastname: Yang
authors.affiliation: Clinical Research Centre, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, ChinaThe Key Immunopharmacology Laboratory of Guangdong Province, Allergy and Inflammation Research Institute, Shantou University Medical College, Shantou 515041, ChinaDepartment of Pathophysiology, Hainan Medical College, Haikou, Hainan 571101, ChinaSchool of Life Sciences, Huaiyin Normal University, Huaiyin, Jiangsu, 223300Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, Canada.
authors.forename: W
authors.initials: W
authors.lastname: Sun
authors.affiliation: Clinical Research Centre, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, ChinaThe Key Immunopharmacology Laboratory of Guangdong Province, Allergy and Inflammation Research Institute, Shantou University Medical College, Shantou 515041, ChinaDepartment of Pathophysiology, Hainan Medical College, Haikou, Hainan 571101, ChinaSchool of Life Sciences, Huaiyin Normal University, Huaiyin, Jiangsu, 223300Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, Canada.
authors.forename: X
authors.initials: X
authors.lastname: Zeng
authors.affiliation: Clinical Research Centre, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, ChinaThe Key Immunopharmacology Laboratory of Guangdong Province, Allergy and Inflammation Research Institute, Shantou University Medical College, Shantou 515041, ChinaDepartment of Pathophysiology, Hainan Medical College, Haikou, Hainan 571101, ChinaSchool of Life Sciences, Huaiyin Normal University, Huaiyin, Jiangsu, 223300Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, Canada.
authors.forename: P
authors.initials: P
authors.lastname: Yang
mesh_terms: D000485:Allergens / Q000276:immunology; D000818:Animals; D052179:Antigens, Plant / Q000276:immunology*; D003058:Cockroaches / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics; D005434:Flow Cytometry; D006801:Humans; D006967:Hypersensitivity; D018793:Interleukin-13 / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D013058:Mass Spectrometry; D008407:Mast Cells / Q000276:immunology; D016133:Polymerase Chain Reaction; D044463:Receptor, PAR-1 / Q000096:biosynthesis; D044464:Receptor, PAR-2 / Q000096:biosynthesis; D044462:Receptors, Proteinase-Activated / Q000096:biosynthesis* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018418:Th2 Cells / Q000378:metabolism
chemical_list: D000485:Allergens; D052179:Antigens, Plant; D018793:Interleukin-13; D044463:Receptor, PAR-1; D044464:Receptor, PAR-2; D044462:Receptors, Proteinase-Activated; C091463:allergen Per a I; D015847:Interleukin-4