title: Activation of AMPK by berberine promotes adiponectin multimerization in 3T3-L1 adipocytes.
abstract: Adiponectin is assembled into trimer (LMW), hexamer (MMW) and high-molecular-weight (HMW) multimer in adipocytes. The HMW adiponectin is more metabolically active and closely associated with peripheral insulin sensitivity. In this study, we reported that berberine, an isoquinoline alkaloid with insulin-sensitizing effect, inhibits the expression of adiponectin, but promotes the assembly of HMW adiponectin and increases the ratio of HMW to total adiponectin. Berberine activates AMPK. Knockdown of AMPKα1 abolishes the effect of berberine. Activation of AMPK by AICAR also increases the level of HMW adiponectin. Our study suggested that activation of AMPK by berberine promotes adiponectin multimerization.
authors.affiliation: MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Li
authors.forename: Pengcheng
authors.initials: P
authors.lastname: Wang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhuang
authors.forename: Huan
authors.initials: H
authors.lastname: Lin
authors.forename: Yehua
authors.initials: Y
authors.lastname: Li
authors.forename: Ling
authors.initials: L
authors.lastname: Liu
authors.forename: Qinghang
authors.initials: Q
authors.lastname: Meng
authors.forename: Ting
authors.initials: T
authors.lastname: Cui
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Zhen
authors.initials: Z
authors.lastname: Li
mesh_terms: D041721:3T3-L1 Cells; D000091162:AMP-Activated Protein Kinase Kinases; D052242:Adiponectin / Q000378:metabolism*; D000818:Animals; D001599:Berberine / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D007333:Insulin Resistance; D051379:Mice; D008970:Molecular Weight; D011494:Protein Kinases / Q000378:metabolism* / Q000502:physiology; D055503:Protein Multimerization / Q000187:drug effects*
chemical_list: D052242:Adiponectin; D001599:Berberine; D011494:Protein Kinases; D000091162:AMP-Activated Protein Kinase Kinases