title: Downregulation of GABA(A) β subunits is transcriptionally controlled by Fmr1p.
abstract: Fragile X mental retardation syndrome is caused by the transcriptional silence of FMR1. Here, a quantitative PCR technique was used to examine the effect of Fmr1p on the expression of GABA(A) β subunits in different mouse brain regions. Our results demonstrated the reduction of GABA(A) β2 mRNA in all brain regions assessed, and the reduction of GABA(A) β3 mRNA in the cortex, suggesting that the expression of GABA(A) β subunits is transcriptionally regulated by Fmr1p. This finding may help to establish the link between the transcriptional profile of the GABAergic inhibitory system and the development of fragile X mental retardation syndrome.
authors.affiliation: Department of Biology, College of Staten Island, City University of New York, 2800 Victory Blvd, Staten Island, NY 10314, USA.
authors.forename: Andrew
authors.initials: A
authors.lastname: Hong
authors.forename: Aiying
authors.initials: A
authors.lastname: Zhang
authors.forename: Yang
authors.initials: Y
authors.lastname: Ke
authors.forename: Abdeslem
authors.initials: A
authors.lastname: El Idrissi
authors.forename: Chang-Hui
authors.initials: CH
authors.lastname: Shen
mesh_terms: D000818:Animals; D020296:Animals, Congenic; D001921:Brain / Q000378:metabolism*; D002531:Cerebellum / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism; D004027:Diencephalon / Q000378:metabolism; D051860:Fragile X Messenger Ribonucleoprotein 1 / Q000235:genetics / Q000502:physiology*; D006624:Hippocampus / Q000378:metabolism; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics*; D009928:Organ Specificity; D021122:Protein Subunits; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D011963:Receptors, GABA-A / Q000096:biosynthesis / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D051860:Fragile X Messenger Ribonucleoprotein 1; D009419:Nerve Tissue Proteins; D021122:Protein Subunits; D012333:RNA, Messenger; D011963:Receptors, GABA-A; C497684:Fmr1 protein, mouse