title: The self-compatibility mechanism in Brassica napus L. is applicable to F1 hybrid breeding.
abstract: Brassica napus, an allopolyploid species having the A genome of B. rapa and the C genome of B. oleracea, is self-compatible, although both B. rapa and B. oleracea are self-incompatible. We have previously reported that SP11/SCR alleles are not expressed in anthers, while SRK alleles are functional in the stigma in B. napus cv. 'Westar', which has BnS-1 similar to B. rapa S-47 and BnS-6 similar to B. oleracea S-15. This genotype is the most frequent S genotype in B. napus, and we hypothesized that the loss of the function of SP11 is the primary cause of the self-compatibility of 'Westar'. To verify this hypothesis, we transformed 'Westar' plants with the SP11 allele of B. rapa S-47. All the transgenic plants and their progeny were completely self-incompatible, demonstrating self-compatibility to be due to the S haplotype having the non-functional SP11 allele in the A genome, which suppresses a functional recessive SP11 allele in the C genome. An artificially synthesized B. napus line having two recessive SP11 alleles was developed by interspecific hybridization between B. rapa and B. oleracea. This line was self-incompatible, but F(1) hybrids between this line and 'Westar' were self-compatible. These results suggest that the self-compatibility mechanism of 'Westar' is applicable to F(1) seed production in B. napus.
authors.affiliation: Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Tochigi
authors.forename: Hisashi
authors.initials: H
authors.lastname: Udagawa
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Kitashiba
authors.forename: Takeshi
authors.initials: T
authors.lastname: Nishio
mesh_terms: D000483:Alleles; D015139:Blotting, Southern; D029688:Brassica napus / Q000235:genetics*; D001947:Breeding; D018628:Gene Dosage; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D006239:Haplotypes; D006824:Hybridization, Genetic; D010940:Plant Proteins / Q000235:genetics; D030821:Plants, Genetically Modified / Q000235:genetics; D011058:Pollen / Q000235:genetics; D011401:Promoter Regions, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D060436:Self-Incompatibility in Flowering Plants
chemical_list: D010940:Plant Proteins