title: Selective biomolecular nanoarrays for parallel single-molecule investigations.
abstract: The ability to direct the self-assembly of biomolecules on surfaces with true nanoscale control is key for the creation of functional substrates. Herein we report the fabrication of nanoscale biomolecular arrays via selective self-assembly on nanopatterned surfaces and minimized nonspecific adsorption. We demonstrate that the platform developed allows for the simultaneous screening of specific protein-DNA binding events at the single-molecule level. The strategy presented here is generally applicable and enables high-throughput monitoring of biological activity in real time and with single-molecule resolution.
authors.affiliation: Department of Applied Physics & Applied Mathematics, Columbia University, New York, New York 10027, USA. mp2766@columbia.edu
authors.forename: Matteo
authors.initials: M
authors.lastname: Palma
authors.forename: Justin J
authors.initials: JJ
authors.lastname: Abramson
authors.forename: Alon A
authors.initials: AA
authors.lastname: Gorodetsky
authors.forename: Erika
authors.initials: E
authors.lastname: Penzo
authors.forename: Ruben L
authors.initials: RL
authors.lastname: Gonzalez
authors.forename: Michael P
authors.initials: MP
authors.lastname: Sheetz
authors.forename: Colin
authors.initials: C
authors.lastname: Nuckolls
authors.forename: James
authors.initials: J
authors.lastname: Hone
authors.forename: Shalom J
authors.initials: SJ
authors.lastname: Wind
mesh_terms: D004247:DNA / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D049329:Nanostructures
chemical_list: D004247:DNA