title: Members of the TEAD family of transcription factors regulate the expression of Myf5 in ventral somitic compartments.
abstract: The transcriptional regulation of the Mrf4/Myf5 locus depends on a multitude of enhancers that, in equilibria with transcription balancing sequences and the promoters, regulate the expression of the two genes throughout embryonic development and in the adult. Transcription in a particular set of muscle progenitors can be driven by the combined outputs of several enhancers that are not able to recapitulate the entire expression pattern in isolation, or by the action of a single enhancer the activity of which in isolation is equivalent to that within the context of the locus. We identified a new enhancer element of this second class, ECR111, which is highly conserved in all vertebrate species and is necessary and sufficient to drive Myf5 expression in ventro-caudal and ventro-rostral somitic compartments in the mouse embryo. EMSA analyses and data obtained from binding-site mutations in transgenic embryos show that a binding site for a TEA Domain (TEAD) transcription factor is essential for the function of this new enhancer, while ChIP assays show that at least two members of the family of transcription factors bind to it in vivo.
authors.affiliation: Section of Gene Function and Regulation, The Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London, SW3 6JB, UK.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Ribas
authors.forename: Natalia
authors.initials: N
authors.lastname: Moncaut
authors.forename: Christine
authors.initials: C
authors.lastname: Siligan
authors.forename: Kevin
authors.initials: K
authors.lastname: Taylor
authors.forename: Joe W
authors.initials: JW
authors.lastname: Cross
authors.forename: Peter W J
authors.initials: PW
authors.lastname: Rigby
authors.forename: Jaime J
authors.initials: JJ
authors.lastname: Carvajal
mesh_terms: D000818:Animals; D001665:Binding Sites / Q000235:genetics; D047369:Chromatin Immunoprecipitation; D004268:DNA-Binding Proteins / Q000378:metabolism*; D024202:Electrophoretic Mobility Shift Assay; D004742:Enhancer Elements, Genetic / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D051379:Mice; D024510:Muscle Development / Q000502:physiology*; D051787:Myogenic Regulatory Factor 5 / Q000378:metabolism*; D019170:Somites / Q000196:embryology* / Q000378:metabolism; D000091262:TEA Domain Transcription Factors; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; C497418:Myf5 protein, mouse; D051787:Myogenic Regulatory Factor 5; D000091262:TEA Domain Transcription Factors; C083215:Tead1 protein, mouse; D014157:Transcription Factors