title: Cellometer vision as an alternative to flow cytometry for cell cycle analysis, mitochondrial potential, and immunophenotyping.
abstract: Cell phenotyping and cell cycle analysis are two commonly used assays in both clinical diagnosis and biomedical research. Cell phenotyping by identifying different biomarkers is essential for the diagnosis of hematologic malignancy, sub-classifying diseases, monitoring response to treatment, predicting prognosis, detecting rare cell populations and residual malignant cells. Cell cycle analysis distinguishes cells in different phases of cell cycle and is often used to determine the cellular response to drugs and biological stimulations. These assays have been traditionally carried out by sensitive fluorescence detection methods such as flow cytometry and laser scanning cytometry for fluorescence-based cell population analysis. However, these instruments remain relatively expensive, large in size, and require a considerable amount of maintenance, which may not be feasible for smaller research groups that do not have access to these equipments or field clinics that require quick diagnostic results on site. Recently, a small portable imaging cytometry system (Cellometer Vision) has been developed by Nexcelom Bioscience LLC (Lawrence, MA) for automated cell concentration and viability measurement using bright-field and fluorescent imaging methods. Here we report new applications of the Cellometer imaging cytometry for fluorescence-based cell population analysis and compared them with conventional flow cytometry. Cell population analysis assays such as immunophenotyping, cell cycle, and mitochondrial membrane potential detection methods have not yet been reported for the Cellometer Vision system. Using this imaging cytometry method for fluorescence-based assays that are typically done by flow cytometry offers a quick, simple, and inexpensive alternative method for biomedical research, which may be beneficial for smaller research laboratories and clinics.
authors.affiliation: Department of Technology R&D, Nexcelom Bioscience LLC, Lawrence, Massachusetts 01843, USA. lchan@nexcelom.com
authors.forename: Leo L
authors.initials: LL
authors.lastname: Chan
authors.forename: Xuemei
authors.initials: X
authors.lastname: Zhong
authors.forename: Jean
authors.initials: J
authors.lastname: Qiu
authors.forename: Peter Y
authors.initials: PY
authors.lastname: Li
authors.forename: Bo
authors.initials: B
authors.lastname: Lin
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000502:physiology*; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods*; D019044:Image Cytometry / Q000295:instrumentation / Q000379:methods*; D016130:Immunophenotyping / Q000379:methods*; D053078:Membrane Potential, Mitochondrial / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000166:cytology; D013950:Thymus Gland / Q000166:cytology