title: Human oncoprotein mdm2 interacts with the tata-binding protein in-vitro and in-vivo.
abstract: Human oncoprotein MDM2 inhibits p53-induced transcriptional activation of promoters containing p53-binding sites. In this report we show that MDM2 interacts with the human TATA binding protein (TBP), in vivo and in. vitro, in the absence of p53. The C-terminal boundary of the TBP-binding domain on MDM2 resides between amino acids 221 and 276, whereas the N-terminal boundary is beyond amino acid 120. Thus, the acidic domain of MDM2 overlaps with the TBP binding domain and is needed for the interaction. The C-terminal conserved domain of TBP is required for MDM2 binding. MDM2-TBP interaction suggests a p53-independent, transcription regulatory role of MDM2.
authors.affiliation: UNIV TEXAS,HLTH SCI CTR,DEPT MICROBIOL,SAN ANTONIO,TX 78284.
authors.forename: P
authors.initials: P
authors.lastname: Leng
authors.forename: D
authors.initials: D
authors.lastname: Brown
authors.forename: S
authors.initials: S
authors.lastname: Deb
authors.forename: S
authors.initials: S
authors.lastname: Deb