title: Backbone and Ile-δ1, Leu, Val methyl 1H, 13C and 15N NMR chemical shift assignments for human interferon-stimulated gene 15 protein.
abstract: Human interferon-stimulated gene 15 protein (ISG15), also called ubiquitin cross-reactive protein (UCRP), is the first identified ubiquitin-like protein containing two ubiquitin-like domains fused in tandem. The active form of ISG15 is conjugated to target proteins via the C-terminal glycine residue through an isopeptide bond in a manner similar to ubiquitin. The biological role of ISG15 is strongly associated with the modulation of cell immune function, and there is mounting evidence suggesting that many viral pathogens evade the host innate immune response by interfering with ISG15 conjugation to both host and viral proteins in a variety of ways. Here we report nearly complete backbone (1)H(N), (15)N, (13)C', and (13)C(α), as well as side chain (13)C(β), methyl (Ile-δ1, Leu, Val), amide (Asn, Gln), and indole N-H (Trp) NMR resonance assignments for the 157-residue human ISG15 protein. These resonance assignments provide the basis for future structural and functional solution NMR studies of the biologically important human ISG15 protein.
authors.affiliation: Department of Molecular Biology and Biochemistry, Center for Advanced Biotechnology and Medicine, Northeast Structural Genomics Consortium, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA.
authors.forename: Cuifeng
authors.initials: C
authors.lastname: Yin
authors.forename: James M
authors.initials: JM
authors.lastname: Aramini
authors.forename: Li-Chung
authors.initials: LC
authors.lastname: Ma
authors.forename: John R
authors.initials: JR
authors.lastname: Cort
authors.forename: G V T
authors.initials: GV
authors.lastname: Swapna
authors.forename: Robert M
authors.initials: RM
authors.lastname: Krug
authors.forename: Gaetano T
authors.initials: GT
authors.lastname: Montelione
mesh_terms: D000595:Amino Acid Sequence; D000597:Amino Acids, Branched-Chain / Q000737:chemistry; D016207:Cytokines / Q000737:chemistry*; D006801:Humans; D007554:Isotopes / Q000737:chemistry; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D011487:Protein Conformation; D014452:Ubiquitins / Q000737:chemistry*
chemical_list: D000597:Amino Acids, Branched-Chain; D016207:Cytokines; D007554:Isotopes; D014452:Ubiquitins; C074034:ISG15 protein, human