title: Recognition of molecularly imprinted polymers for a quaternary alkaloid of berberine.
abstract: Selective and affinitive imprinted polymers incorporating a quaternary alkaloid of berberine (BER) were prepared using a non-covalent imprinting method. The results showed that, compared to other imprinted polymers, the polymer AD-10 had not only a higher of the ratio of Q(MIP)/Q(BP) for BER adsorption, and but also a larger of the ratio of Q(MIP,B)/Q(MIP,P) for BER and palmatine (PAL) adsorptions. Spectrophotometric analysis demonstrated that a 1:1 cooperative hydrogen-bonding complex might be predominating in the pre-polymerization between the BER template and AA monomer. Adsorption experiments of BER on the polymer AD-10 were in accordance with the second-order and Langmuir adsorption models. The E value (5.70 kJ/mol) calculated from the Dubinin-Radushkevich model indicated that the adsorption followed a physisorption process. In addition, a Scatchard plot showed a single straight line with an equilibrium dissociation constant (K(D)) of 65.80 μmol/L. SPE analyses of a mixture of BER and PAL and the methanol extract from the cortices of Phellodendron wilsonii showed that AD-10 had more efficiency, and higher specificity and selectivity for SPE in the concentration and determination of BER and its extraction from natural products.
authors.affiliation: Material and Chemical Research Laboratories, Industrial Technology Research Institute, Hsinchu 31040, Taiwan, ROC.
authors.forename: Chia-Yun
authors.initials: CY
authors.lastname: Chen
authors.forename: Chih-Hung
authors.initials: CH
authors.lastname: Wang
authors.forename: Arh-Hwang
authors.initials: AH
authors.lastname: Chen
mesh_terms: D000178:Acrylamides / Q000737:chemistry; D000327:Adsorption; D001599:Berberine / Q000737:chemistry* / Q000302:isolation & purification; D007700:Kinetics; D008689:Methacrylates / Q000737:chemistry; D000432:Methanol / Q000737:chemistry; D054802:Molecular Imprinting / Q000379:methods*; D032102:Phellodendron / Q000737:chemistry; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry*; D016062:Porosity; D052616:Solid Phase Extraction; D013499:Surface Properties
chemical_list: D000178:Acrylamides; D008689:Methacrylates; D011108:Polymers; D001599:Berberine; C008384:methacrylic acid; C004919:ethylene dimethacrylate; D000432:Methanol