title: Kinesin I ATPase manipulates biohybrids formed from tubulin and carbon nanotubes.
abstract: This chapter describes a method for the formation of novel protein-nanotube hybrid conjugates. Specifically, we took advantage of the self-assembly and self-recognition properties of tubulin cytoskeletal protein immobilized onto carbon nanotubes to form nanotube-based biohybrids. Further biohybrid hierarchical integration in assemblies enabled molecular-level manipulation on engineered surfaces, as demonstrated with biocatalyst kinesin 1 ATPase molecular motor. The method presented herein can be extended for the preparation of biocatalyst-based or protein-based assemblies to be used as sensors or biological templates for nanofabrication.
authors.affiliation: Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, Center for Biotechnology and Interdisciplinary Studies, Troy, NY, USA. cerasela-zoica.dinu@mail.wvu.edu
authors.forename: Cerasela Zoica
authors.initials: CZ
authors.lastname: Dinu
authors.forename: Shyam Sundhar
authors.initials: SS
authors.lastname: Bale
authors.forename: Jonathan S
authors.initials: JS
authors.lastname: Dordick
mesh_terms: D000818:Animals; D001710:Biotin / Q000032:analysis; D002417:Cattle; D004330:Drosophila; D005453:Fluorescence; D055767:Immobilized Proteins / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure*; D016547:Kinesins / Q000378:metabolism* / Q000494:pharmacology; D046529:Microscopy, Electron, Transmission; D009068:Movement / Q000187:drug effects; D058726:Nanoconjugates / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D012235:Rhodamines / Q000032:analysis; D014404:Tubulin / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure*
chemical_list: D055767:Immobilized Proteins; D058726:Nanoconjugates; D037742:Nanotubes, Carbon; D012235:Rhodamines; D014404:Tubulin; D001710:Biotin; D016547:Kinesins