title: Fate of bone marrow stromal cells in a syngenic model of bone formation.
abstract: Bone marrow stromal cells (BMSCs) have been demonstrated to induce bone formation when associated to osteoconductive biomaterials and implanted in vivo. Nevertheless, their role in bone reconstruction is not fully understood and rare studies have been conducted to follow their destiny after implantation in syngenic models. The aim of the present work was to use sensitive and quantitative methods to track donor and recipient cells after implantation of BMSCs in a syngenic model of ectopic bone formation. Using polymerase chain reaction (PCR) amplification of the Sex determining Region Y (Sry) gene and in situ hybridization of the Y chromosome in parallel to histological analysis, we have quantified within the implants the survival of the donor cells and the colonization by the recipient cells. The putative migration of the BMSCs in peripheral organs was also analyzed. We show here that grafted cells do not survive more than 3 weeks after implantation and might migrate in peripheral lymphoid organs. These cells are responsible for the attraction of host cells within the implants, leading to the centripetal colonization of the biomaterial by new bone.
authors.affiliation: GEPITOS, Université Nice Sophia-Antipolis, CNRS, UFR de Médecine, Nice, France.
authors.forename: Florian
authors.initials: F
authors.lastname: Boukhechba
authors.forename: Thierry
authors.initials: T
authors.lastname: Balaguer
authors.forename: Sébastien
authors.initials: S
authors.lastname: Bouvet-Gerbettaz
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Michiels
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Bouler
authors.forename: Georges F
authors.initials: GF
authors.lastname: Carle
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Scimeca
authors.forename: Nathalie
authors.initials: N
authors.lastname: Rochet
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D002465:Cell Movement / Q000235:genetics / Q000502:physiology; D005260:Female; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010012:Osteogenesis / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051876:Sex-Determining Region Y Protein / Q000235:genetics; D017154:Stromal Cells / Q000166:cytology* / Q000378:metabolism; D014998:Y Chromosome / Q000235:genetics
chemical_list: D051876:Sex-Determining Region Y Protein