title: Effect of dinitrosyl iron complexes with glutathione on hemorrhagic shock followed by saline treatment.
abstract: It has been found that dinitrosyl iron complexes with glutathione (DNIC-GS) injected into the blood flow of rats at a dose of 0.05 μmoles/kg prior to hemorrhage significantly improve cardiac function under conditions of hemorrhagic shock manifested in increased stroke volume, left ventricular work and cardiac output to a level exceeding control values 1.5-fold. Enhanced myocardial contractile activity leads to a situation where mean arterial pressure does not decrease further despite the significant decrease of total peripheral resistance. The decrease of total peripheral vascular resistance of the vascular system under vasodilating effects of DNIC-GS used as nitric oxide donors improves microcirculation in experimental rats judging from increased rates of blood flow and low degree of erythrocyte aggregation. Pretreatment of rats with the complexes significantly increases survival (by 21%) under conditions of hemorrhagic shock. It is suggested that beneficial effects of DNIC-GS on systemic circulation parameters under conditions of hemorrhagic shock are determined by their antioxidant activity and the ability to induce S-nitrosylation of proteins.
authors.affiliation: Russian Research Institute of Hematology and Transfusiology, Russian Ministry of Public Health, Saint-Petersburg, Russia.
authors.forename: Marina I
authors.initials: MI
authors.lastname: Remizova
authors.forename: Nikolai I
authors.initials: NI
authors.lastname: Kochetygov
authors.forename: Konstantin A
authors.initials: KA
authors.lastname: Gerbout
authors.forename: Vladimir L
authors.initials: VL
authors.lastname: Lakomkin
authors.forename: Alexander A
authors.initials: AA
authors.lastname: Timoshin
authors.forename: Evgenia N
authors.initials: EN
authors.lastname: Burgova
authors.forename: Anatoly F
authors.initials: AF
authors.lastname: Vanin
mesh_terms: D000818:Animals; D001775:Blood Circulation / Q000187:drug effects; D001794:Blood Pressure / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D005978:Glutathione / Q000737:chemistry*; D007501:Iron / Q000737:chemistry* / Q000494:pharmacology* / Q000627:therapeutic use; D009589:Nitrogen Oxides / Q000737:chemistry* / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D012771:Shock, Hemorrhagic / Q000188:drug therapy* / Q000503:physiopathology; D012965:Sodium Chloride / Q000494:pharmacology*; D016019:Survival Analysis
chemical_list: D009589:Nitrogen Oxides; D012965:Sodium Chloride; C073661:dinitrosyl iron complex; D007501:Iron; D005978:Glutathione