title: RNA versus DNA (NucliSENS EasyQ HIV-1 v1.2 versus Amplicor HIV-1 DNA test v1.5) for early diagnosis of HIV-1 infection in infants in Senegal.
abstract: The objective of this study was to compare the performance of the NucliSENS EasyQ HIV-1 v1.2 platform (bioMérieux, France) to the Amplicor HIV-1 DNA test v1.5 (Roche Molecular Systems, Switzerland) in detecting HIV-1 infection in infants using venipuncture-derived whole blood in tubes and dried blood spots. A total of 149 dried blood spots and 43 EDTA-anticoagulated peripheral blood samples were collected throughout Dakar and other areas in Senegal from infants and children aged 3 weeks to 24 months who were born to HIV-1-infected mothers. Samples were tested using the NucliSENS and Amplicor technologies. The NucliSENS and Amplicor results were 100% concordant using either EDTA-anticoagulated peripheral blood or dried blood spots. Compared to Amplicor, the sensitivity and specificity of the NucliSENS test were 100%. The NucliSENS EasyQ HIV-1 RNA assay performed as well as the Amplicor HIV-1 DNA test in detecting HIV-1 infection in infants. In addition, this platform can give an indication of the viral load baseline. The NucliSENS EasyQ platform is a good alternative for early infant diagnosis of HIV-1 infection.
authors.affiliation: Laboratoire de Bactériologie Virologie, Hôpital Aristide le Dantec, Université Cheikh Anta Diop, Dakar, Senegal.
authors.forename: K
authors.initials: K
authors.lastname: Kébé
authors.forename: O
authors.initials: O
authors.lastname: Ndiaye
authors.forename: H Diop
authors.initials: HD
authors.lastname: Ndiaye
authors.forename: P Mbakob
authors.initials: PM
authors.lastname: Mengue
authors.forename: P M M
authors.initials: PM
authors.lastname: Guindo
authors.forename: S
authors.initials: S
authors.lastname: Diallo
authors.forename: N
authors.initials: N
authors.lastname: Léye
authors.forename: S B
authors.initials: SB
authors.lastname: Gueye
authors.forename: A Gaye
authors.initials: AG
authors.lastname: Diallo
authors.forename: C Touré
authors.initials: CT
authors.lastname: Kane
authors.forename: S
authors.initials: S
authors.lastname: Mboup
mesh_terms: D004279:DNA, Viral / Q000097:blood / Q000235:genetics / Q000302:isolation & purification*; D003890:Desiccation; D042241:Early Diagnosis; D015658:HIV Infections / Q000175:diagnosis*; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D012367:RNA, Viral / Q000097:blood / Q000235:genetics / Q000302:isolation & purification*; D011933:Reagent Kits, Diagnostic; D012675:Senegal; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000379:methods; D014773:Virology / Q000379:methods*
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral; D011933:Reagent Kits, Diagnostic