title: Brazilian Bidens pilosa Linné yields fraction containing quercetin-derived flavonoid with free radical scavenger activity and hepatoprotective effects.
abstract: Bidens pilosa is a plant used by Amazonian and Asian folks for some hepatopathies. The hydroethanol crude extract and three fractions were assessed for antioxidant and hepatoprotective effects. Higher levels of scavenger activity on the 1,1-diphenyl-2-picrylhydrazyl radical, inhibition of deoxyribose oxidation and lipid peroxidation in vitro were detected for the ethyl acetate fraction (IC(50)~4.3-32.3 µg/ml) followed by the crude extract (IC(50)~14.2-98.0 µg/ml). The ethyl acetate fraction, again followed by the crude extract, showed high contents of total soluble polyphenols (3.6±0.2 and 2.1±0.2 GAE/mg, respectively) and presence of a quercetin-derived flavonoid identified as quercetin 3,3'-dimethyl ether 7-O-β-D-glycopyranoside. Both products were assayed for hepatoprotector effects against CCl(4)-induced liver injury in mice. Markers of oxidative stress and hepatic injury were evaluated. The results showed that the 10-day pretreatments (15 mg/kg, p.o.) protected the livers against injury by blocking CCl(4)-induced lipid peroxidation and protein carbonylation and the DNA fragmentation was decreased (~60%). The pretreatments avoided the loss of the plasma ferric reducing/antioxidant power and the elevation of serum transaminases and lactate dehydrogenase activities. The results suggest that the main constituents responsible for the hepatoprotective effects with free radical scavenger power associated are well extracted by performing fractionation with ethyl acetate. The findings support the Brazilian traditional use of this plant and justify further evaluations for the therapeutic efficacy and safety of the constituents of the ethyl acetate fraction to treat some liver diseases.
authors.affiliation: Departamento de Bioquímica, Universidade Federal de Santa Catarina, Florianópolis, Brazil.
authors.forename: Maicon Roberto
authors.initials: MR
authors.lastname: Kviecinski
authors.forename: Karina Bettega
authors.initials: KB
authors.lastname: Felipe
authors.forename: João Francisco Gomes
authors.initials: JF
authors.lastname: Correia
authors.forename: Eduardo Antonio
authors.initials: EA
authors.lastname: Ferreira
authors.forename: Maria Helena
authors.initials: MH
authors.lastname: Rossi
authors.forename: Fernando
authors.initials: F
authors.lastname: de Moura Gatti
authors.forename: Danilo Wilhelm
authors.initials: DW
authors.lastname: Filho
authors.forename: Rozangela Curi
authors.initials: RC
authors.lastname: Pedrosa