title: Enzymatic synthesis of capsaicin 4-O-β-xylooligosaccharides by β-xylosidase from Aspergillus sp.
abstract: Capsaicin 4-O-β-xylooligosaccharides were synthesized by a biocatalytic xylosylation using Aspergillus sp. β-xylosidase. Capsaicin was converted into three new capsaicin glycosides, i.e. capsaicin 4-O-β-xyloside, capsaicin 4-O-β-xylobioside, and capsaicin 4-O-β-xylotrioside in 15, 12 and 10% yield, respectively. All products were isolated from the reaction mixtures by preparative HPLC. The structures of the products were determined by NMR spectroscopic method.
authors.affiliation: Sunny Health Co. Ltd., Yaesu Chuo-ku, Tokyo 103-0028 Japan.
authors.forename: Hisashi
authors.initials: H
authors.lastname: Katsuragi
authors.forename: Kei
authors.initials: K
authors.lastname: Shimoda
authors.forename: Ryohei
authors.initials: R
authors.lastname: Yamamoto
authors.forename: Takahiro
authors.initials: T
authors.lastname: Ohara
authors.forename: Hiroki
authors.initials: H
authors.lastname: Hamada
mesh_terms: D001230:Aspergillus / Q000201:enzymology*; D002211:Capsaicin / Q000138:chemical synthesis*; D002851:Chromatography, High Pressure Liquid; D006027:Glycosides / Q000138:chemical synthesis; D006031:Glycosylation; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D009844:Oligosaccharides / Q000138:chemical synthesis*; D014995:Xylosidases / Q000378:metabolism*
chemical_list: D006027:Glycosides; D009844:Oligosaccharides; C010807:xylosides; D014995:Xylosidases; C026579:exo-1,4-beta-D-xylosidase; D002211:Capsaicin