title: High-yields heterologous production of the novel Aspergillus fumigatus elastase inhibitor AFUEI in Aspergillus oryzae.
abstract: AFUEI, an elastase inhibitor produced by Aspergillus fumigatus strongly inhibits the elastolytic activity of A. fumigatus etc. To purify AFUEI, we constructed a strain that overproduces AFUEI by introducing the gene encoding AFUEI (Genbank accession no. AB546725) under control of the amyB promoter into the heterologous host Aspergillus oryzae. A. oryzae TF-4 displayed strong elastase inhibitory activity and produced considerably more AFUEI than that of A. fumigatus. Furthermore, AFUEI could be purified using culture broth and single ultrafiltration (UF) treatment, allowing for the effective production of AFUEI for use in clinical trials.
authors.affiliation: Research & Development Department, Hakutsuru Sake Brewing Co. Ltd, 4-5-5 Sumiyoshiminami-machi, Higashinada-ku, Kobe 658-0041, Japan. yamashita@hakutsuru.co.jp
authors.forename: Nobuo
authors.initials: N
authors.lastname: Yamashita
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Komori
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Okumura
authors.forename: Kei-Ichi
authors.initials: K
authors.lastname: Uchiya
authors.forename: Takeshi
authors.initials: T
authors.lastname: Matsui
authors.forename: Akira
authors.initials: A
authors.lastname: Nishimura
authors.forename: Kenji
authors.initials: K
authors.lastname: Ogawa
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Nikai
mesh_terms: D000935:Antifungal Agents / Q000378:metabolism* / Q000494:pharmacology; D001232:Aspergillus fumigatus / Q000201:enzymology*; D001236:Aspergillus oryzae / Q000235:genetics*; D002478:Cells, Cultured; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology; D006801:Humans; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology; D015842:Serine Proteinase Inhibitors / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology
chemical_list: D000935:Antifungal Agents; D005656:Fungal Proteins; D011994:Recombinant Proteins; D015842:Serine Proteinase Inhibitors; D010196:Pancreatic Elastase