title: Atomic force microscopy study of ganglioside GM1 concentration effect on lateral phase separation of sphingomyelin/dioleoylphosphatidylcholine/cholesterol bilayers.
abstract: The effect of monosialoganglioside GM1 (GM1) concentration on the lateral phase separation in the sphingomyelin/1,2-dioleoyl-sn-glycero-3-phosphocholine/cholesterol (SM/DOPC/Chol) bilayers was studied by using atomic force microscopy. The results show that, with the increase of GM1 mol fraction (x), the dominant composition of liquid-ordered (L(o)) domains changes from SM to SM/GM1 and finally to GM1. Meanwhile, the decrease of domain area (A) of the L(o) phase with the increase of x follows a scaling law of A ∼ x(-3/2), for x > 0.005, indicating that the domain growth is pinned with high GM1 concentration. Results of in situ experiments of GM1 insertion into SM/DOPC/cholesterol bilayers further supported our observations.
authors.affiliation: The Key Laboratory of Molecular Engineering of Polymers, Ministry of Education, Department of Macromolecular Science, the Centre of Analysis and Measurement, Fudan University, Shanghai 200433, PR China.
authors.forename: Ren
authors.initials: R
authors.lastname: Bao
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Feng
authors.initials: F
authors.lastname: Qiu
authors.forename: Yuliang
authors.initials: Y
authors.lastname: Yang
mesh_terms: D002784:Cholesterol / Q000737:chemistry*; D005677:G(M1) Ganglioside / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D008560:Membrane Fluidity; D018625:Microscopy, Atomic Force; D010713:Phosphatidylcholines / Q000737:chemistry*; D013109:Sphingomyelins / Q000737:chemistry*
chemical_list: D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D013109:Sphingomyelins; D005677:G(M1) Ganglioside; D002784:Cholesterol; C017251:1,2-oleoylphosphatidylcholine