title: Intrapleural delivery of mesenchymal stem cells: a novel potential treatment for pleural diseases.
abstract: OBJECTIVE
  To develop a method to deliver mesenchymal stem cells (MSCs) into the pleural cavity for the treatment of pleural diseases.
  
  
  METHODS
  MSCs were isolated from rat bone marrow of rats and labeled with 4',6-diamidino-2-phenylindole dihydrochloride (DAPI) or green fluorescent protein (GFP) using a lentiviral vector. Eighteen Sprague-Dawley (SD) rats were inoculated intrapleurally with 1×10(6) MSCs-DAPI. The distribution of the fluorescent cells was observed using fluorescent microscopy for the following 30 d. Another 12 rats inoculated intrapleurally with 1×10(6) MSCs-GFP were observed for 14 d.
  
  
  RESULTS
  The isolated cells were typical MSC phenotypes and could differentiate into adipocytes, osteoblasts, and chondroblasts in vitro. Microscopic analysis revealed that the labeled cells adhered to the surface of the pleural cavity. The highest number of the labeled cells was found to be adhered to all specimens from the mediastinal pleura, but no labeled cells were detected in the lung parenchyma or other tissues/organs, such as the liver, kidney, spleen, and mesenterium. Incidentally, stomas were found in the mediastinal pleura. The recovered MSCs-GFP from the pleural cavity retained their ability to adhere and proliferate.
  
  
  CONCLUSIONS
  We have established a novel method for intrapleural delivery of MSCs. The distribution of intrapleurally delivered MSCs was found to be limited to the pleurae and the pleural cavity, thereby providing us with a new approach to further investigation of the therapeutic roles of MSCs in pleural diseases.
authors.affiliation: Department of Pulmonary Medicine, Huadong Hospital, Shanghai Medical College, Fudan University, Shanghai 200040, China.
authors.forename: Zhao-hui
authors.initials: ZH
authors.lastname: Qin
authors.forename: Jie-ming
authors.initials: JM
authors.lastname: Qu
authors.forename: Jin-fu
authors.initials: JF
authors.lastname: Xu
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Hanssa
authors.initials: H
authors.lastname: Summah
authors.forename: He-xi Ge
authors.initials: HX
authors.lastname: Sai-Yin
authors.forename: Chun-mei
authors.initials: CM
authors.lastname: Chen
authors.forename: Long
authors.initials: L
authors.lastname: Yu
mesh_terms: D017667:Adipocytes / Q000378:metabolism; D000818:Animals; D002448:Cell Adhesion; D002454:Cell Differentiation; D019902:Chondrocytes / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007211:Indoles / Q000378:metabolism; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D010006:Osteoblasts / Q000378:metabolism; D010995:Pleural Diseases / Q000628:therapy*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013194:Staining and Labeling
chemical_list: D007211:Indoles; D049452:Green Fluorescent Proteins; C007293:DAPI