title: Heteronuclear single-quantum coherence nuclear magnetic resonance (HSQC NMR) characterization of acetylated fir (Abies sachallnensis MAST) wood regenerated from ionic liquid.
abstract: An ionic liquid, 1-butyl-3-methylimidazolium chloride ([Bmim]Cl), was used to dissolve Japanese fir (Abies sachallnensis MAST) wood. Milled woods prepared by planetary ball-milling for 8 h dissolved completely in [Bmim]Cl at 100 °C in 2 h. The dissolved woods were then subjected to in situ acetylation, and the fully acetylated woods were regenerated from [Bmim]Cl. (1)H-(13)C correlation heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) experiments were successfully conducted with the acetylated woods in dimethyl sulfoxide (DMSO)-d(6). The acetylated lignin and polysaccharide signals dispersed reasonably well on the 2D spectra. Characterization of the NMR signals for the whole cell-wall components, including lignin, cellulose, and hemicelluloses, was achieved by comparison with isolated lignin and commercial cellulose and hemicelluloses (arabinoxylan, galactomannan, and glucomannan). The procedure used here is applicable for the characterization of cell-wall components in various plant biomasses.
authors.affiliation: Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, Imizu, Japan.
authors.forename: Chen
authors.initials: C
authors.lastname: Qu
authors.forename: Takao
authors.initials: T
authors.lastname: Kishimoto
authors.forename: Masanori
authors.initials: M
authors.lastname: Kishino
authors.forename: Masahiro
authors.initials: M
authors.lastname: Hamada
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Nakajima
mesh_terms: D028202:Abies; D000107:Acetylation; D002473:Cell Wall / Q000737:chemistry; D002482:Cellulose / Q000032:analysis; D004121:Dimethyl Sulfoxide; D052578:Ionic Liquids; D008031:Lignin / Q000032:analysis; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D011134:Polysaccharides / Q000032:analysis; D014934:Wood / Q000737:chemistry*
chemical_list: D052578:Ionic Liquids; D011134:Polysaccharides; C007916:hemicellulose; D002482:Cellulose; D008031:Lignin; D004121:Dimethyl Sulfoxide