title: Immunoprecipitation of piRNPs and directional, next generation sequencing of piRNAs.
abstract: Piwi interacting RNAs (piRNAs) are small (∼25 to ∼30 nucleotide) and are expressed in the germline. piRNAs bind to the Piwi subclade of Argonaute proteins and form the core ribonucleoproteins (RNPs) of piRNPs. We describe a method for the massive identification of piRNAs from immunopurified piRNPs. This strategy may also be used for immunopurification and directional sequencing of RNAs from other RNPs that contain small RNAs.
authors.affiliation: Division of Neuropathology, Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA, USA.
authors.forename: Yohei
authors.initials: Y
authors.lastname: Kirino
authors.forename: Anastassios
authors.initials: A
authors.lastname: Vourekas
authors.forename: Eugene
authors.initials: E
authors.lastname: Khandros
authors.forename: Zissimos
authors.initials: Z
authors.lastname: Mourelatos
mesh_terms: D000818:Animals; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism; D005260:Female; D059014:High-Throughput Nucleotide Sequencing; D047468:Immunoprecipitation; D008297:Male; D051379:Mice; D016133:Polymerase Chain Reaction; D034741:RNA, Small Interfering / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D014982:Xenopus laevis / Q000235:genetics / Q000378:metabolism
chemical_list: D034741:RNA, Small Interfering