title: Biodistribution study of nanometric hybrid gadolinium oxide particles as a multimodal SPECT/MR/optical imaging and theragnostic agent.
abstract: Nanometric hybrid gadolinium oxide particles (Gado-6Si-NP) for diagnostic and therapeutic applications (mean diameter 3-4 nm) were obtained by encapsulating Gd(2)O(3) cores within a polysiloxane shell, which carries organic fluorophore (Cy 5) and is derivatized by a hydrophilic carboxylic layer. As residency time in the living body and methods of waste elimination are crucial to defining a good nanoparticle candidate and moving forward with steps for validation, this study was aimed at evaluating the biodistribution of these multimodal Gado-6Si-NP in rodents. Gado-6Si-NP were imaged following intravenous injection in control Wistar rats and mice using MRI (7 T), optical fluorescent imaging, and SPECT. A clear correlation was observed among MRI, optical imaging, and SPECT regarding the renal elimination. Quantitative biodistribution using gamma-counting of each sampled organ confirmed that these nanoparticles circulated freely in the blood pool and were rapidly cleared by renal excretion without accumulation in liver and RES uptake. These results demonstrate that Gado-6Si-NP display optimal biodistribution properties, enabling them to be developed as multimodal agents for in vivo imaging and theragnostics, especially in oncological applications.
authors.affiliation: Hospices Civils de Lyon, Lyon, France. david.kryza@chu-lyon.fr
authors.forename: David
authors.initials: D
authors.lastname: Kryza
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Taleb
authors.forename: Marc
authors.initials: M
authors.lastname: Janier
authors.forename: Laurence
authors.initials: L
authors.lastname: Marmuse
authors.forename: Imen
authors.initials: I
authors.lastname: Miladi
authors.forename: Pauline
authors.initials: P
authors.lastname: Bonazza
authors.forename: Cédric
authors.initials: C
authors.lastname: Louis
authors.forename: Pascal
authors.initials: P
authors.lastname: Perriat
authors.forename: Stéphane
authors.initials: S
authors.lastname: Roux
authors.forename: Olivier
authors.initials: O
authors.lastname: Tillement
authors.forename: Claire
authors.initials: C
authors.lastname: Billotey
mesh_terms: D000818:Animals; D005453:Fluorescence; D005682:Gadolinium / Q000493:pharmacokinetics* / Q000627:therapeutic use*; D007205:Indium Radioisotopes; D007668:Kidney / Q000378:metabolism; D008279:Magnetic Resonance Imaging; D057054:Molecular Imaging; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D051381:Rats; D017208:Rats, Wistar; D012825:Silicon / Q000737:chemistry*; D012995:Solubility; D013499:Surface Properties; D014018:Tissue Distribution; D015899:Tomography, Emission-Computed, Single-Photon
chemical_list: D007205:Indium Radioisotopes; C030581:gadolinium oxide; D005682:Gadolinium; D012825:Silicon