title: Endomicroscopy imaging of epithelial structures using tissue autofluorescence.
abstract: We explore autofluorescence endomicroscopy as a potential tool for real-time visualization of epithelial tissue microstructure and organization in a clinical setting. The design parameters are explored using two experimental systems--an Olympus Medical Systems Corp. stand-alone clinical prototype probe, and a custom built bench-top rigid fiber conduit prototype. Both systems entail ultraviolet excitation at 266 nm and/or 325 nm using compact laser sources. Preliminary results using ex vivo animal and human tissue specimens suggest that this technology can be translated toward in vivo application to address the need for real-time histology.
authors.affiliation: University of California, Davis, NSF Center for Biophotonics Science & Technology, 4800 2nd Avenue, Sacramento, California 95817, USA.
authors.forename: Bevin
authors.initials: B
authors.lastname: Lin
authors.forename: Shiro
authors.initials: S
authors.lastname: Urayama
authors.forename: Ramez M G
authors.initials: RM
authors.lastname: Saroufeem
authors.forename: Dennis L
authors.initials: DL
authors.lastname: Matthews
authors.forename: Stavros G
authors.initials: SG
authors.lastname: Demos
mesh_terms: D000818:Animals; D001471:Barrett Esophagus / Q000473:pathology; D004724:Endoscopy / Q000295:instrumentation / Q000379:methods; D004867:Equipment Design; D005753:Gastric Mucosa / Q000737:chemistry / Q000166:cytology; D006651:Histocytochemistry / Q000295:instrumentation / Q000379:methods; D006801:Humans; D007668:Kidney / Q000737:chemistry / Q000166:cytology; D051379:Mice; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet