title: A rapid DNA extraction method for PCR amplification from wetland soils.
abstract: OBJECTIVE
  We tested a method of rapid DNA extraction from wetland soil samples for use in the polymerase chain reaction.
  
  
  RESULTS
  The glass bead/calcium chloride/SDS method obtained in the present study was compared with the calcium chloride/SDS/enzymatic extraction method and the UltraClean™ Soil DNA Isolation Kit. Rapid DNA extraction could be completed within about two hours without purification steps.
  
  
  CONCLUSIONS
  This study succeeded in establishing a fast soil DNA extraction protocol that can be applied to various environmental sources that are rich in humic acid content.
  
  
  CONCLUSIONS
  The method provides a technology with high-quality DNA extraction from soils for testing the diversity of AOB and AOA.
authors.affiliation: College of Life Sciences, Inner Mongolia University, Huhhot, China.
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.forename: B
authors.initials: B
authors.lastname: Li
authors.forename: Y
authors.initials: Y
authors.lastname: Zhou
authors.forename: J
authors.initials: J
authors.lastname: Xu
authors.forename: J
authors.initials: J
authors.lastname: Zhao
mesh_terms: D000641:Ammonia / Q000378:metabolism; D001105:Archaea / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D019641:DNA, Archaeal / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification*; D005821:Genetic Techniques; D006812:Humic Substances / Q000032:analysis; D008828:Microbiological Techniques; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000235:genetics; D012987:Soil / Q000737:chemistry*; D012988:Soil Microbiology; D053833:Wetlands
chemical_list: D019641:DNA, Archaeal; D004269:DNA, Bacterial; D006812:Humic Substances; D012336:RNA, Ribosomal, 16S; D012987:Soil; D000641:Ammonia