title: Detection of three-base deletion by exciplex formation with perylene derivatives.
abstract: Here, we synthesized fluorescent DNA probes labeled with two perylene derivatives for the detection of a three-base deletion mutant. One such probe discriminated the three-base deletion mutant from the wild-type sequence by exciplex emission, and the deletion mutant was identifiable even by the naked eye.
authors.affiliation: Graduate School of Engineering, Nagoya University, Furocho, Nagoya 464-8603, Japan.
authors.forename: Hiromu
authors.initials: H
authors.lastname: Kashida
authors.forename: Nobuyo
authors.initials: N
authors.lastname: Kondo
authors.forename: Koji
authors.initials: K
authors.lastname: Sekiguchi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Asanuma
mesh_terms: D001483:Base Sequence; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000235:genetics; D015342:DNA Probes / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D017353:Gene Deletion; D006801:Humans; D009154:Mutation; D010569:Perylene / Q000737:chemistry*; D011110:Polymorphism, Genetic; D013056:Spectrophotometry, Ultraviolet
chemical_list: D015342:DNA Probes; D005456:Fluorescent Dyes; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D010569:Perylene