title: Molecular authentication of geo-authentic Scrophularia ningpoensis.
abstract: Scrophularia ningpoensis has long been used in the Chinese Materia Medica for inflammation. Like other herbal medicines, S. ningpoensis collected from different localities may considerably differ in their therapeutic efficacy, and the one grown in Zhejiang Province is recognized as geo-authentic. However, it is difficult to confirm the geographical authenticity by similar morphological characteristics. In the present study, inter-simple sequence repeat (ISSR) markers were conducted to detect S. ningpoensis from different origins. A 1 259-bp fragment amplified by primer UBC874 was found only in geo-authentic ones. By cloning and sequencing that specific band, sequence characterized amplified region (SCAR) markers were designed to distinguish geo-authentic S. ningpoensis from others. This is a rapid and easy method that can be used to identify the geographical authenticity of S. ningpoensis.
authors.affiliation: Key Laboratory of Conservation Biology for Endangered Wildlife of Ministry of Education, College of Life Sciences, Zhejiang University, Hangzhou, China.
authors.forename: Chuan
authors.initials: C
authors.lastname: Chen
authors.forename: Li-na
authors.initials: LN
authors.lastname: Duan
authors.forename: Xiao-long
authors.initials: XL
authors.lastname: Zhou
authors.forename: Bing-long
authors.initials: BL
authors.lastname: Chen
authors.forename: Cheng-xin
authors.initials: CX
authors.lastname: Fu
mesh_terms: D001483:Base Sequence; D002681:China; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000592:standards; D005819:Genetic Markers; D008516:Medicine, Chinese Traditional; D018598:Minisatellite Repeats; D010946:Plants, Medicinal / Q000235:genetics*; D016133:Polymerase Chain Reaction; D032266:Scrophularia / Q000235:genetics*
chemical_list: D017931:DNA Primers; D018744:DNA, Plant; D004365:Drugs, Chinese Herbal; D005819:Genetic Markers