title: Genomic and resistance gene homolog diversity of the dominant tallgrass prairie species across the U.S. Great Plains precipitation gradient.
abstract: BACKGROUND
  Environmental variables such as moisture availability are often important in determining species prevalence and intraspecific diversity. The population genetic structure of dominant plant species in response to a cline of these variables has rarely been addressed. We evaluated the spatial genetic structure and diversity of Andropogon gerardii populations across the U.S. Great Plains precipitation gradient, ranging from approximately 48 cm/year to 105 cm/year.
  
  
  RESULTS
  Genomic diversity was evaluated with AFLP markers and diversity of a disease resistance gene homolog was evaluated by PCR-amplification and digestion with restriction enzymes. We determined the degree of spatial genetic structure using Mantel tests. Genomic and resistance gene homolog diversity were evaluated across prairies using Shannon's index and by averaging haplotype dissimilarity. Trends in diversity across prairies were determined using linear regression of diversity on average precipitation for each prairie. We identified significant spatial genetic structure, with genomic similarity decreasing as a function of distance between samples. However, our data indicated that genome-wide diversity did not vary consistently across the precipitation gradient. In contrast, we found that disease resistance gene homolog diversity was positively correlated with precipitation.
  
  
  CONCLUSIONS
  Prairie remnants differ in the genetic resources they maintain. Selection and evolution in this disease resistance homolog is environmentally dependent. Overall, we found that, though this environmental gradient may not predict genomic diversity, individual traits such as disease resistance genes may vary significantly.
authors.affiliation: Department of Plant Pathology, Kansas State University, Manhattan, Kansas, United States of America.
authors.forename: Matthew N
authors.initials: MN
authors.lastname: Rouse
authors.forename: Amgad A
authors.initials: AA
authors.lastname: Saleh
authors.forename: Amadou
authors.initials: A
authors.lastname: Seck
authors.forename: Kathleen H
authors.initials: KH
authors.lastname: Keeler
authors.forename: Steven E
authors.initials: SE
authors.lastname: Travers
authors.forename: Scot H
authors.initials: SH
authors.lastname: Hulbert
authors.forename: Karen A
authors.initials: KA
authors.lastname: Garrett
mesh_terms: D001483:Base Sequence; D044822:Biodiversity; D017931:DNA Primers; D017343:Genes, Plant; D015146:Midwestern United States; D011003:Ploidies; D006109:Poaceae / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011891:Rain
chemical_list: D017931:DNA Primers