title: A role for GPN-loop GTPase yGPN1 in sister chromatid cohesion.
abstract: Sister chromatid cohesion and separation, involving the cohesin complex, are crucial for accurate inheritance of genetic information. This complex is also fundamental for efficient post-replicative repair of DNA double-strand breaks and has a key role in the mechanisms of gene transcription control. Cohesin is subjected to many post-translational modifications but the regulators implicated in the control of its activity have been poorly described. Here, we show that the conserved and essential GPN loop GTPase yGPN1 in Saccharomyces cerevisiae is involved in sister chromatid cohesion mechanisms. Based on a sister chromatid cohesion assay, we found that over-expression of the yGPN1 gene promotes sister chromatid separation during anaphase. The sharp slowdown in progression of the S phase observed in cells where yGPN1 expression is down-regulated strongly suggests that yGPN1 is necessary for DNA replication. Moreover, analysis of yGPN1 protein-protein interaction network highlights the yGPN1 links with DNA replication, sister chromatid cohesion/separation and the gene expression process.
authors.affiliation: Laboratoire de Biochimie des Systèmes Perturbés, Bagnols-sur-Cèze, France.
authors.forename: Béatrice
authors.initials: B
authors.lastname: Alonso
authors.forename: Guylaine
authors.initials: G
authors.lastname: Chaussinand
authors.forename: Jean
authors.initials: J
authors.lastname: Armengaud
authors.forename: Christian
authors.initials: C
authors.lastname: Godon
mesh_terms: D018797:Cell Cycle Proteins / Q000502:physiology*; D002842:Chromatids / Q000502:physiology*; D002868:Chromosomal Proteins, Non-Histone / Q000502:physiology*; D004261:DNA Replication; D020558:GTP Phosphohydrolases / Q000502:physiology*; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D016196:S Phase; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000201:enzymology* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D012854:Sister Chromatid Exchange; D000097722:Cohesins
chemical_list: D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D020558:GTP Phosphohydrolases; D006023:Glycoproteins; D029701:Saccharomyces cerevisiae Proteins; D000097722:Cohesins; C086475:YGP1 protein, S cerevisiae