title: Complete genome sequence of Metallosphaera cuprina, a metal sulfide-oxidizing archaeon from a hot spring.
abstract: The genome of the metal sulfide-oxidizing, thermoacidophilic strain Metallosphaera cuprina Ar-4 has been completely sequenced and annotated. Originally isolated from a sulfuric hot spring, strain Ar-4 grows optimally at 65°C and a pH of 3.5. The M. cuprina genome has a 1,840,348-bp circular chromosome (2,029 open reading frames [ORFs]) and is 16% smaller than the previously sequenced Metallosphaera sedula genome. Compared to the M. sedula genome, there are no counterpart genes in the M. cuprina genome for about 480 ORFs in the M. sedula genome, of which 243 ORFs are annotated as hypothetical protein genes. Still, there are 233 ORFs uniquely occurring in M. cuprina. Genome annotation supports that M. cuprina lives a facultative life on CO(2) and organics and obtains energy from oxidation of sulfidic ores and reduced inorganic sulfuric compounds.
authors.affiliation: Institute of Microbiology, Beijing, People's Republic of China.
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Liu
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: You
authors.forename: Huajun
authors.initials: H
authors.lastname: Zheng
authors.forename: Shengyue
authors.initials: S
authors.lastname: Wang
authors.forename: Cheng-Ying
authors.initials: CY
authors.lastname: Jiang
authors.forename: Shuang-Jiang
authors.initials: SJ
authors.lastname: Liu
mesh_terms: D002876:Chromosomes, Bacterial; D019641:DNA, Archaeal / Q000737:chemistry* / Q000235:genetics*; D020745:Genome, Archaeal; D045482:Hot Springs / Q000382:microbiology; D006863:Hydrogen-Ion Concentration; D008670:Metals / Q000378:metabolism; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D017422:Sequence Analysis, DNA; D013440:Sulfides / Q000378:metabolism; D016943:Sulfolobaceae / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism; D013696:Temperature
chemical_list: D019641:DNA, Archaeal; D008670:Metals; D013440:Sulfides