title: Haemocyte protein expression profiling of scallop Chlamys farreri response to acute viral necrosis virus (AVNV) infection.
abstract: Acute viral necrosis virus (AVNV) was newly reported as one causative agent responsible for mass mortality of adult Chinese scallop Chlamys farreri, which is widely cultured on northern China coast. Unfortunately, the interaction between virus and host is largely unknown. According to these, this study was undertaken to deeply explore the immune response of haemocyte against AVNV. Two-dimensional gel electrophoresis (2-DE) was introduced to produce protein expression profiles from samples taken at 24h post-infection (hpi) from the haemocytes of C. farreri that were either specific pathogen free or else infected with AVNV. Forty-eight protein spots, which consistently showed either a marked change (≥1.5-fold difference) in accumulated levels or else were highly expressed in haemocytes, were selected for further investigation. In-gel trypsin digestion was conducted followed by matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI-TOF-MS). Matching search was subsequently performed throughout bioinformatics databases. A total of 42 proteins were identified, all of which were classified into eight categories according to their Gene Ontology annotations of biological processes and molecular functions, i.e. cytoskeleton proteins, proteins involved in metabolism, proteins related to calcium homeostasis, chaperone, proteins involved in immunity, proteins involved in transcriptional regulation, proteins related to signal transduction, and ungrouped proteins. The possible biological significance of some observed proteins in the host response to AVNV was discussed. These studies could be served as the first global analysis of differentially expressed proteins in haemocytes from AVNV-infected C. farreri, and in addition to increasing our understanding of the pathogenesis of this virus-associated scallop disease, the results presented here should be useful both for potential biomarkers identification and anti-virus approaches development as well.
authors.affiliation: College of Oceanology, Harbin Institute of Technology (Weihai), Weihai 264209, PR China.
authors.forename: Guofu
authors.initials: G
authors.lastname: Chen
authors.forename: Chunyun
authors.initials: C
authors.lastname: Zhang
authors.forename: Chenghua
authors.initials: C
authors.lastname: Li
authors.forename: Chongming
authors.initials: C
authors.lastname: Wang
authors.forename: Zhong
authors.initials: Z
authors.lastname: Xu
authors.forename: Peisheng
authors.initials: P
authors.lastname: Yan
mesh_terms: D000818:Animals; D015870:Gene Expression; D020869:Gene Expression Profiling; D006434:Hemocytes / Q000201:enzymology / Q000276:immunology* / Q000378:metabolism*; D054884:Host-Pathogen Interactions; D049897:Pectinidae / Q000201:enzymology / Q000276:immunology* / Q000821:virology*; D015398:Signal Transduction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014780:Viruses / Q000276:immunology*