title: Phages of Pseudomonas aeruginosa: response to environmental factors and in vitro ability to inhibit bacterial growth and biofilm formation.
abstract: OBJECTIVE
  To examine effects of various environmental factors on adsorption and inactivation of Pseudomonas aeruginosa-specific phages: δ (family Podoviridae), J-1, σ-1 and 001A (family Siphoviridae) and their ability to inhibit bacterial growth and biofilm formation.
  
  
  RESULTS
  The phages examined in the study were clonally different, as revealed by RFLP. The temperature in the range 7-44°C had no influence on the adsorption of Podoviridae, but did affect Siphoviridae adsorption, particularly 001A. All phages were significantly stable at pH 5-9, and phages δ and 001A even at pH 3. Most of the examined carbohydrates and exopolysaccharides of the original host efficiently inactivated phage δ, while phages σ-1 and J-1 were inactivated considerably only by the amino acid alanine. Silver nitrate efficiently inactivated all the phages, while Siphoviridae were more resistant to povidone-iodine. Serum of nonimmunized rats had no influence on phage inactivation and adsorption. Only phage δ showed ability to effectively inhibit in vitro bacterial growth and biofilm formation.
  
  
  CONCLUSIONS
  The examined environmental parameters can significantly influence the adsorption and viability of Ps. aeruginosa-specific phages. The phage δ is a good candidate for biocontrol of Ps. aeruginosa.
  
  
  CONCLUSIONS
  The study provides important data on Ps. aeruginosa-specific phage adsorption, inactivation and in vitro lytic efficacy.
authors.affiliation: Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad, Novi Sad, Vojvodina, Serbia. petar.knezevic@dbe.uns.ac.rs
authors.forename: P
authors.initials: P
authors.lastname: Knezevic
authors.forename: D
authors.initials: D
authors.lastname: Obreht
authors.forename: S
authors.initials: S
authors.lastname: Curcin
authors.forename: M
authors.initials: M
authors.lastname: Petrusic
authors.forename: V
authors.initials: V
authors.lastname: Aleksic
authors.forename: R
authors.initials: R
authors.lastname: Kostanjsek
authors.forename: O
authors.initials: O
authors.lastname: Petrovic
mesh_terms: D000818:Animals; D001433:Bacteriolysis; D018441:Biofilms; D005260:Female; D017902:Podoviridae / Q000235:genetics / Q000502:physiology; D017105:Pseudomonas Phages / Q000145:classification / Q000235:genetics / Q000502:physiology*; D011550:Pseudomonas aeruginosa / Q000502:physiology* / Q000821:virology*; D051381:Rats; D017208:Rats, Wistar; D017906:Siphoviridae / Q000235:genetics / Q000502:physiology; D013696:Temperature