title: Roles of conserved arginines in ATP-binding domains of AAA+ chaperone ClpB from Thermus thermophilus.
abstract: ClpB, a member of the expanded superfamily of ATPases associated with diverse cellular activities (AAA+), forms a ring-shaped hexamer and cooperates with the DnaK chaperone system to reactivate aggregated proteins in an ATP-dependent manner. The ClpB protomer consists of an N-terminal domain, an AAA+ module (AAA-1), a middle domain, and a second AAA+ module (AAA-2). Each AAA+ module contains highly conserved WalkerA and WalkerB motifs, and two arginines (AAA-1) or one arginine (AAA-2). Here, we investigated the roles of these arginines (Arg322, Arg323, and Arg747) of ClpB from Thermus thermophilus in the ATPase cycle and chaperone function by alanine substitution. These mutations did not affect nucleotide binding, but did inhibit the hydrolysis of the bound ATP and slow the threading of the denatured protein through the central pore of the T. thermophilus ClpB ring, which severely impaired the chaperone functions. Previously, it was demonstrated that ATP binding to the AAA-1 module induced motion of the middle domain and stabilized the ClpB hexamer. However, the arginine mutations of the AAA-1 module destabilized the ClpB hexamer, even though ATP-induced motion of the middle domain was not affected. These results indicated that the three arginines are crucial for ATP hydrolysis and chaperone activity, but not for ATP binding. In addition, the two arginines in AAA-1 and the ATP-induced motion of the middle domain independently contribute to the stabilization of the hexamer.
authors.affiliation: Department of Biology, Faculty of Science and Engineering, Konan University, Okamoto, Kobe, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Yamasaki
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Nakazaki
authors.forename: Masasuke
authors.initials: M
authors.lastname: Yoshida
authors.forename: Yo-hei
authors.initials: YH
authors.lastname: Watanabe
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism*; D001120:Arginine / Q000378:metabolism*; D008958:Models, Molecular; D018832:Molecular Chaperones / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D016963:Thermus thermophilus / Q000235:genetics / Q000378:metabolism*
chemical_list: D018832:Molecular Chaperones; D000255:Adenosine Triphosphate; D001120:Arginine; D000251:Adenosine Triphosphatases