title: Radiation induced G2/M block and apoptosis in two human sarcoma cell lines with different p53 gene status.
abstract: We investigated a wild-type (wt) p53 rhabdomyosarcoma (A-204) and a mutated (mt p53) undifferentiated sarcoma cell line (US8-93) for their response to X-rays. The observation period was 0 to 96 h after irradiation. Both cell lines showed a strikingly delayed G2/M arrest and an induction of apoptosis after irradiation. Compared with the cell line A-204 (wt p53), the cell line US8-93 (mt p53) revealed a stronger G2/M arrest. In agreement with this, in terms of viability as well as the rate of apoptosis, A-204 (wt p53) showed a stronger response to irradiation than US8-93 (mt p53). We suggest that the different p53 gene status might be the cause for a different response to irradiation.
authors.affiliation: UNIV HALLE WITTENBERG,INST PATHOL,D-060979 HALLE,GERMANY. UNIV HALLE WITTENBERG,DEPT RADIOTHERAPY,HALLE,GERMANY. UNIV HALLE WITTENBERG,CLIN GEN SURG,HALLE,GERMANY. UNIV HALLE WITTENBERG,DEPT DERMATOL,HALLE,GERMANY.
authors.forename: M
authors.initials: M
authors.lastname: Bache
authors.forename: J
authors.initials: J
authors.lastname: Dunst
authors.forename: P
authors.initials: P
authors.lastname: Wurl
authors.forename: D
authors.initials: D
authors.lastname: Frode
authors.forename: M
authors.initials: M
authors.lastname: Dietzel
authors.forename: A
authors.initials: A
authors.lastname: Meye
authors.forename: H
authors.initials: H
authors.lastname: Schmidt
authors.forename: F
authors.initials: F
authors.lastname: Rath
authors.forename: W
authors.initials: W
authors.lastname: Wohlrab
authors.forename: H
authors.initials: H
authors.lastname: Dralle
authors.forename: H
authors.initials: H
authors.lastname: Taubert