title: Dynamic of oxidative and nitrosative stress markers during the convalescent period of stroke patients undergoing rehabilitation.
abstract: BACKGROUND
  Stroke patients have a redox imbalance, a consequence of both the cerebrovascular event and the associated pathological conditions. Our study was aimed to investigate the dynamic of some oxidative and nitrosative markers during the convalescent phase of postacute stroke patients undergoing rehabilitation.
  
  
  METHODS
  We assessed thiol, advanced oxidation protein product, protein carbonyl, 3-nitro-l-tyrosine, ceruloplasmin and oxidized LDL concentrations, as well as gamma-glutamyltranspeptidase (GGT) activity in 20 patients at the beginning of the hospitalization and at the discharge moment, respectively, and 24 apparently healthy controls.
  
  
  RESULTS
  We found significantly increased values for GGT (P = 0.04), ceruloplasmin (P = 0.01) and protein carbonyl (P = 0.04) in stroke patients at the hospitalization moment when compared with healthy controls, while total thiols were significantly decreased (P = 0.002). Rehabilitation was associated with a significant decrease of protein carbonyl (P = 0.03) and oxidized LDL particle concentrations (P = 0.03), as well as GGT activity (P = 0.02). At the hospitalization moment, both GGT and ceruloplasmin were significantly negatively correlated with non-proteic thiols (r = -0.44, P = 0.049, and r = -0.53, P = 0.015, respectively) and significantly positively with protein carbonyls (r = +0.80, P < 0.001, and r = +0.69, P < 0.001, respectively) suggesting putative roles of GGT and ceruloplasmin in the redox imbalance.
  
  
  CONCLUSIONS
  These results highlight the existence of a redox imbalance in postacute stroke patients, and the possible benefits of an antioxidant-based therapy for the recovery of these patients.
authors.affiliation: Department of Biochemistry, University of Medicine and Pharmacy Carol Davila, 020022 Bucharest, Romania. manolescub@yahoo.fr
authors.forename: Bogdan Nicolae
authors.initials: BN
authors.lastname: Manolescu
authors.forename: M
authors.initials: M
authors.lastname: Berteanu
authors.forename: E
authors.initials: E
authors.lastname: Oprea
authors.forename: N
authors.initials: N
authors.lastname: Chiriac
authors.forename: L
authors.initials: L
authors.lastname: Dumitru
authors.forename: S
authors.initials: S
authors.lastname: Vladoiu
authors.forename: O
authors.initials: O
authors.lastname: Popa
authors.forename: O
authors.initials: O
authors.lastname: Ianas
mesh_terms: D000368:Aged; D015415:Biomarkers / Q000097:blood; D002570:Ceruloplasmin / Q000032:analysis; D003289:Convalescence; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009584:Nitrogen / Q000378:metabolism*; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D020521:Stroke / Q000097:blood*; D000071939:Stroke Rehabilitation; D013438:Sulfhydryl Compounds / Q000097:blood; D005723:gamma-Glutamyltransferase / Q000097:blood
chemical_list: D015415:Biomarkers; D013438:Sulfhydryl Compounds; D002570:Ceruloplasmin; D005723:gamma-Glutamyltransferase; C554782:gamma-glutamyltransferase, human; D009584:Nitrogen