title: Increasing manganese peroxidase productivity of Phanerochaete chrysosporium by optimizing carbon sources and supplementing small molecules.
abstract: OBJECTIVE
  To overproduce manganese peroxidase (MnP) using Phanerochaete chrysosporium. Effects of carbon sources, agricultural by-products and small molecules were tested to enhance the MnP productivity.
  
  
  RESULTS
  Various carbon sources and agricultural by-products were compared as the basal medium. A MnP activity of 4.7 ± 0.31 U ml⁻¹ was obtained using mannose as a carbon source. The enzyme productivity further reached 7.36 ± 0.05 and 8.77 ± 0.23 U ml⁻¹ when the mannose medium was supplemented with cyclic adenosine monophosphate (cAMP) and S-adenosylmethionine, respectively.
  
  
  CONCLUSIONS
  This study revealed the highest MnP productivity obtained by optimizing the carbon sources and supplementing small molecules. It can provide insight for: (i) making high quantities of enzymes by optimizing media resources and (ii) engineering the global regulatory genes in P. chrysosporium for the onsite production of MnP.
  
  
  CONCLUSIONS
  As MnP is an important enzyme to hydrolyse lignin polymers which protects the plant cell wall from exposing of cellulose fibres, the production of the enzyme is a current demand for biofuel biotechnology. The knowledge generated from this study can help to advance the technology for stable production of MnP.
authors.affiliation: Department of Biological Systems Engineering, The Bioprocessing and Bioproduct Engineering Laboratory (BBEL), Washington State University, Pullman, WA, USA.
authors.forename: D
authors.initials: D
authors.lastname: Singh
authors.forename: J
authors.initials: J
authors.lastname: Zeng
authors.forename: S
authors.initials: S
authors.lastname: Chen
mesh_terms: D002244:Carbon / Q000378:metabolism; D007218:Industrial Microbiology; D010544:Peroxidases / Q000096:biosynthesis*; D020075:Phanerochaete / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism
chemical_list: D002244:Carbon; D010544:Peroxidases; C051129:manganese peroxidase