title: Evaluation, using extracted human teeth, of Ricetral as a storage medium for avulsions--an in vitro study.
abstract: The prognosis of teeth replanted following avulsion is determined by the extra-alveolar time and storage medium used. This study was undertaken to determine the efficacy of an oral rehydration solution 'Ricetral', in retaining the vitality of periodontal ligament cells when used as a storage medium for avulsed teeth prior to replantation. The study consisted of a comparative evaluation between Ricetral and two currently recommended solutions, Hank's balanced salt solution (HBSS) and milk. Thirty extracted teeth were dried for 30min and soaked in the respective storage media for 45min. The periodontal ligament cells were isolated by an enzyme treatment with collagenase and trypsin. The cells were evaluated for vitality by trypan blue staining and number of vital cells counted in a hemocytometer. Statistical analysis revealed that cell vitality was high with Ricetral and HBSS, but poor with milk.
authors.affiliation: Department of Conservative Dentistry and Endodontics, Amrita School of Dentistry, Kochi, Kerala, India. priyar@aims.amrita.edu
authors.forename: Priya
authors.initials: P
authors.lastname: Rajendran
authors.forename: Nettiyat Oommen
authors.initials: NO
authors.lastname: Varghese
authors.forename: Jolly Mary
authors.initials: JM
authors.lastname: Varughese
authors.forename: Elango
authors.initials: E
authors.lastname: Murugaian
mesh_terms: D000818:Animals; D002452:Cell Count; D018929:Cell Culture Techniques; D002470:Cell Survival / Q000187:drug effects; D004396:Coloring Agents; D003890:Desiccation; D006801:Humans; D007552:Isotonic Solutions / Q000627:therapeutic use; D008422:Materials Testing; D008892:Milk; D019188:Organ Preservation Solutions / Q000627:therapeutic use*; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects*; D015532:Rehydration Solutions / Q000627:therapeutic use*; D013997:Time Factors; D014084:Tooth Avulsion / Q000473:pathology*; D014343:Trypan Blue
chemical_list: D004396:Coloring Agents; C072670:Hanks Balanced Salt Solution; D007552:Isotonic Solutions; D019188:Organ Preservation Solutions; D015532:Rehydration Solutions; D014343:Trypan Blue