title: Aquaporin-4 expression after experimental contusional injury in an ovine impact-acceleration head injury model.
abstract: Cerebral contusions are one of the principal lesions of traumatic brain injury and the attendant oedema formation contributes substantially to the clinicopathological manifestations. While it is now recognised that the membrane channel protein aquaporin-4 (AQP-4) plays a major role in the development and resolution of cerebral oedema, assessments of its expression in and around contusions have produced conflicting results. We used an ovine impact-acceleration model of closed head injury to examine contusion-related AQP-4 expression and found that there was a heterogeneous AQP-4 response within contusions, with some astrocytes being nonviable and immunonegative, while others showed increased AQP-4 expression. Pericontusional astrocytes in the penumbra region generally showed more robust AQP-4 immunopositivity than intracontusional glia. Thus, manipulation of AQP-4 expression could have therapeutic applications in controlling cerebral oedema associated with contusions.
authors.affiliation: SA Pathology, Hanson Institute Centre for Neurological Diseases, Institute of Medical and Veterinary Science, PO Box 14 Rundle Mall, Adelaide, South Australia 5000, Australia.
authors.forename: John W
authors.initials: JW
authors.lastname: Finnie
authors.forename: Peter C
authors.initials: PC
authors.lastname: Blumbergs
authors.forename: Jim
authors.initials: J
authors.lastname: Manavis
mesh_terms: D000818:Animals; D051401:Aquaporin 4 / Q000096:biosynthesis*; D001253:Astrocytes / Q000378:metabolism / Q000473:pathology; D001929:Brain Edema / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D001930:Brain Injuries / Q000150:complications / Q000378:metabolism* / Q000473:pathology*; D004195:Disease Models, Animal; D005260:Female; D016489:Head Injuries, Closed / Q000378:metabolism / Q000473:pathology; D007150:Immunohistochemistry; D012756:Sheep
chemical_list: D051401:Aquaporin 4