title: Effect of short hairpin RNA of Bcl-xL gene on biological behaviors of HepG-2 cells.
abstract: To investigate the inhibitory effect of short hairpin RNA (shRNA) targeting the Bcl-xL gene on the apoptosis and proliferation of hepatocellular carcinoma HepG-2 cells. We constructed Bcl-xL shRNA, and transfected it into HepG-2 cells, then detected Bcl-xL gene expression on mRNA and protein levels by reverse transcription-polymerase chain reaction (RT-PCR) and flow cytometry, respectively. Cell proliferation was detected by MTT assay. Cell apoptosis and Bcl-2 and Bax expression were detected by flow cytometry. The expression of Bcl-xL was obviously inhibited by RNA interference. The inhibition rates of Bcl-xL mRNA and protein were 86.6 and 70.2%, respectively. Bcl-xL shRNA inhibited cell proliferation (P < 0.05), whereas promoted apoptosis (P < 0.01). After transfection of Bcl-xL shRNA in HepG-2 cells, the expression of Bcl-2 did not change, but Bax increased significantly. Bcl-xL shRNA effectively inhibits Bcl-xL gene expression and proliferation of HepG-2 cells and promotes apoptosis.
authors.affiliation: Department of Clinical Laboratory, The First Affiliated Hospital of Dalian Medical University, Dalian 116011, China.
authors.forename: Duan
authors.initials: D
authors.lastname: Mengxi
authors.forename: Yuan
authors.initials: Y
authors.lastname: Hong
authors.forename: Wang
authors.initials: W
authors.lastname: Nan
authors.forename: Li
authors.initials: L
authors.lastname: Shijun
authors.forename: Jiang
authors.initials: J
authors.lastname: Yanmei
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D049109:Cell Proliferation; D005434:Flow Cytometry; D056945:Hep G2 Cells; D006801:Humans; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics*; D034741:RNA, Small Interfering / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051020:bcl-X Protein / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism
chemical_list: C494811:BCL2L1 protein, human; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D051020:bcl-X Protein