title: Rapid detection of a cocaine-binding aptamer using biological nanopores on a chip.
abstract: This paper describes a methodology for the rapid and highly selective detection of cocaine using a membrane protein channel combined with a DNA aptamer. The DNA aptamer recognizes the cocaine molecule with high selectivity. We successfully detected a low concentration of cocaine (300 ng/mL, the drug test cutoff limit) within 60 s using a biological nanopore embedded in a microchip.
authors.affiliation: Bio Microsystems Project, Kanagawa Academy of Science and Technology (KAST), Kawasaki City, Japan.
authors.forename: Ryuji
authors.initials: R
authors.lastname: Kawano
authors.forename: Toshihisa
authors.initials: T
authors.lastname: Osaki
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Sasaki
authors.forename: Masahiro
authors.initials: M
authors.lastname: Takinoue
authors.forename: Satoko
authors.initials: S
authors.lastname: Yoshizawa
authors.forename: Shoji
authors.initials: S
authors.lastname: Takeuchi
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000378:metabolism; D015374:Biosensing Techniques; D003042:Cocaine / Q000032:analysis* / Q000378:metabolism; D008954:Models, Biological; D015394:Molecular Structure; D058608:Nanopores; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D013997:Time Factors
chemical_list: D052157:Aptamers, Nucleotide; D003042:Cocaine