title: The CTGF -945GC polymorphism is not associated with plasma CTGF and does not predict nephropathy or outcome in type 1 diabetes.
abstract: The -945GC polymorphism (rs6918698) in the connective tissue growth factor gene promoter (CTGF/CCN-2) has been associated with end organ damage in systemic sclerosis. Because CTGF is important in progression of diabetic kidney disease, we investigated whether the -945GC polymorphism is associated with plasma CTGF level and outcome in type 1 diabetes. The study cohort consisted of 448 diabetic nephropathy patients and 419 normoalbuminuric diabetic patients with complete data concerning renal function and cardiovascular characteristics. Genomic DNA was genotyped by a QPCR-based SNP assay. We observed no relation between the -945GC polymorphism and plasma CTGF level, and the genotype frequencies were not different in nephropathy patients vs. normoalbuminuric controls. General and cardiovascular mortality, and renal function decline was similar in patients with CC, CG or GG genotypes. In conclusion, the -945GC SNP does not affect plasma CTGF levels, incidence and prognosis of diabetic nephropathy, and cardiovascular outcome.
authors.affiliation: Department of Pathology, University Medical Center Utrecht, Heidelberglaan 100, 3584CX Utrecht, The Netherlands.
authors.forename: Amélie
authors.initials: A
authors.lastname: Dendooven
authors.forename: Tri Q
authors.initials: TQ
authors.lastname: Nguyen
authors.forename: Lodewijk
authors.initials: L
authors.lastname: Brosens
authors.forename: Dongxia
authors.initials: D
authors.lastname: Li
authors.forename: Lise
authors.initials: L
authors.lastname: Tarnow
authors.forename: Hans-Henrik
authors.initials: HH
authors.lastname: Parving
authors.forename: Peter
authors.initials: P
authors.lastname: Rossing
authors.forename: Roel
authors.initials: R
authors.lastname: Goldschmeding
mesh_terms: D000328:Adult; D015331:Cohort Studies; D055513:Connective Tissue Growth Factor / Q000097:blood* / Q000235:genetics*; D003922:Diabetes Mellitus, Type 1 / Q000150:complications* / Q000235:genetics*; D003928:Diabetic Nephropathies / Q000209:etiology*; D018450:Disease Progression; D005838:Genotype; D006801:Humans; D007677:Kidney Function Tests; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide
chemical_list: C526317:CCN2 protein, human; D055513:Connective Tissue Growth Factor