title: Proteome changes in the insoluble protein fraction of bovine Longissimus dorsi muscle as a result of low-voltage electrical stimulation.
abstract: Changes induced by low-voltage electrical stimulation (ES; 0-95 V for 8 s; 95 V for 32 s) in the insoluble protein fraction of bovine longissimus dorsi (LD) muscle at 1 and 24h post-ES were investigated by proteomics. Protein abundance patterns from ten Norwegian Red (NRF) young bulls were compared, and significant changes due to ES were found by rotation test and partial least square (PLS) regression analyses. Five protein spots showed lower abundance in ES samples at both sampling times, and in addition, 10 proteins at 1 h post-ES and 13 proteins at 24 h post-ES changed significantly in abundance due to ES. Reduced abundance of full-length structural proteins in ES samples indicates an accelerated proteolysis due to ES. Moreover, increased abundance of small heat shock proteins indicates earlier initiation of stress responses due to ES. These findings provide a better understanding of the biochemical processes taking place as a result of ES during post mortem storage of meat.
authors.affiliation: Nofima Mat AS, Osloveien 1, NO-1430 Ås, Norway.
authors.forename: Stefanía Guðrún
authors.initials: SG
authors.lastname: Bjarnadóttir
authors.forename: Kristin
authors.initials: K
authors.lastname: Hollung
authors.forename: Martin
authors.initials: M
authors.lastname: Høy
authors.forename: Eva
authors.initials: E
authors.lastname: Veiseth-Kent
mesh_terms: D000199:Actins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D002417:Cattle; D003402:Creatine Kinase / Q000378:metabolism; D003893:Desmin / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D004558:Electric Stimulation / Q000379:methods; D015180:Electrophoresis, Gel, Two-Dimensional; D005519:Food Preservation / Q000379:methods; D006360:Heat-Shock Proteins / Q000032:analysis; D008297:Male; D013058:Mass Spectrometry; D008460:Meat; D018482:Muscle, Skeletal / Q000737:chemistry*; D009210:Myofibrils / Q000737:chemistry; D020543:Proteome / Q000032:analysis* / Q000378:metabolism; D040901:Proteomics; D020107:Troponin T / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D038201:alpha-Crystallins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism
chemical_list: D000199:Actins; D003893:Desmin; D006360:Heat-Shock Proteins; D020543:Proteome; D020107:Troponin T; D038201:alpha-Crystallins; D003402:Creatine Kinase