title: Aging-related alterations in the expression and distribution of GluR2 and PICK1 in the rat hippocampus.
abstract: Deficit in synaptic plasticity in the hippocampus frequently occurs during normal aging. Although the protein level and calcium permeability of AMPARs alter with aging, the alteration of AMPARs and their regulatory proteins during aging are far from understanding. Dynamics of GluR2 subunit are dependent on the function of protein interacting with Cα kinase 1 (PICK1), PKCα and calcineurin (CaN). Here, we firstly show that the expression of PICK1 and CaN B decreased significantly in the hippocampus of old rats compared to that of young and adult rats. The decrease was accompanied by a reduction of GluR2 and PKCα and an increase in CaN A. Next, we found that in young and adult rats, the distribution of PICK1 and GluR2 diffused in the cytoplasm of hippocampal neurons, but closely around perinuclear in the hippocampal neurons of old rats. These results suggest that the expression of GluR2, PICK1, PKCα and CaN B significant decreased in the hippocampus and these alterations may lead to altered distribution of GluR2 and PICK1 during aging.
authors.affiliation: Department of Pharmacology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Dan-Fang
authors.initials: DF
authors.lastname: Yu
authors.forename: Peng-Fei
authors.initials: PF
authors.lastname: Wu
authors.forename: Hui
authors.initials: H
authors.lastname: Fu
authors.forename: Jin
authors.initials: J
authors.lastname: Cheng
authors.forename: Yuan-Jian
authors.initials: YJ
authors.lastname: Yang
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
authors.forename: Li-Hong
authors.initials: LH
authors.lastname: Long
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Chen
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D015153:Blotting, Western; D019703:Calcineurin / Q000096:biosynthesis; D002352:Carrier Proteins / Q000096:biosynthesis*; D003598:Cytoskeletal Proteins; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D020869:Gene Expression Profiling; D006624:Hippocampus / Q000378:metabolism*; D008297:Male; D009687:Nuclear Proteins / Q000096:biosynthesis*; D011493:Protein Kinase C / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D018091:Receptors, AMPA / Q000096:biosynthesis*
chemical_list: D002352:Carrier Proteins; D003598:Cytoskeletal Proteins; D009687:Nuclear Proteins; C498298:PICK1 protein, rat; D018091:Receptors, AMPA; D011493:Protein Kinase C; D019703:Calcineurin; C104722:glutamate receptor ionotropic, AMPA 2