title: Metallation of ethylenediamine based Schiff base with biologically active Cu(II), Ni(II) and Zn(II) ions: synthesis, spectroscopic characterization, electrochemical behaviour, DNA binding, photonuclease activity and in vitro antimicrobial efficacy.
abstract: A new ligand [C28H20N6O8] (L2) has been synthesized by the condensation reaction of 3-hydroxy-4-nitrobenzaldehydenephenylhydrazine (L1) with diethyloxalate. This ligand L2 is allowed to react with bis(ethylenediamine)Cu(II)/Ni(II)/Zn(II) complexes. It affords [(L2)Cu(en)2]Cl2(1)/[(L2)Ni(en)2]Cl2(2)/[(L2)Zn(en)2]Cl2(3) complexes, respectively. These complexes (1-3) have been characterized by the spectral and analytical techniques. The interaction of these complexes with calf thymus (CT) DNA is characterized by the absorption spectra which exhibit a slight red shift with hypochromic effect. Electrochemical analyses and viscosity measurements have also been carried out to determine the mode of binding. The shift in ΔEp, E1/2 and Ipc values explores the interaction of CT DNA with the above metal complexes. The slight increase in the viscosity of CT DNA indicates that these complexes bind to CT DNA through a partial non-classical intercalative mode. Cleavage experiments using pBR322 DNA in presence of H2O2 indicate that these complexes behave as efficient artificial chemical nucleases in the order of 1>2>3. Moreover, the antibacterial and antifungal studies reveal that complex 1 is highly active against the bacterial and fungal growth.
authors.affiliation: Research Department of Chemistry, VHNSN College, Virudhunagar, Tamil Nadu 626 001, India. drn_raman@yahoo.co.in
authors.forename: N
authors.initials: N
authors.lastname: Raman
authors.forename: A
authors.initials: A
authors.lastname: Selvan
authors.forename: S
authors.initials: S
authors.lastname: Sudharsan
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000494:pharmacology*; D000935:Antifungal Agents / Q000138:chemical synthesis / Q000494:pharmacology*; D002417:Cattle; D002712:Chlorides / Q000737:chemistry; D003300:Copper / Q000737:chemistry; D004247:DNA / Q000378:metabolism*; D004563:Electrochemistry; D004578:Electron Spin Resonance Spectroscopy; D005029:Ethylenediamines / Q000737:chemistry*; D006861:Hydrogen Peroxide; D008024:Ligands; D015394:Molecular Structure; D009532:Nickel / Q000737:chemistry; D009942:Organometallic Compounds / Q000138:chemical synthesis* / Q000494:pharmacology*; D010777:Photochemistry; D012545:Schiff Bases; D021241:Spectrometry, Mass, Electrospray Ionization; D014961:X-Ray Diffraction; D017967:Zinc Compounds / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D002712:Chlorides; D005029:Ethylenediamines; D008024:Ligands; D009942:Organometallic Compounds; D012545:Schiff Bases; D017967:Zinc Compounds; C031234:ethylenediamine; C022838:nickel chloride; D003300:Copper; D009532:Nickel; C016837:zinc chloride; D004247:DNA; C427203:calf thymus DNA; D006861:Hydrogen Peroxide; C028419:cuprous chloride