title: New microbial mannan catabolic pathway that involves a novel mannosylglucose phosphorylase.
abstract: The consecutive genes BF0771-BF0774 in the genome of Bacteroides fragilis NCTC 9343 were found to constitute an operon. The functional analysis of BF0772 showed that the gene encoded a novel enzyme, mannosylglucose phosphorylase that catalyzes the reaction, 4-O-β-d-mannopyranosyl-d-glucose+Pi→mannose-1-phosphate+glucose. Here we propose a new mannan catabolic pathway in the anaerobe, which involves 1,4-β-mannanase (BF0771), a mannobiose and/or sugar transporter (BF0773), mannobiose 2-epimerase (BF0774), and mannosylglucose phosphorylase (BF0772), finally progressing to glycolysis. This pathway is distributed in microbes such as Bacteroides, Parabacteroides, Flavobacterium, and Cellvibrio.
authors.affiliation: Creative Research Initiative 'Sousei', Hokkaido University, N-21 W-10 Kita-ku, Sapporo, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Senoura
authors.forename: Shigeaki
authors.initials: S
authors.lastname: Ito
authors.forename: Hidenori
authors.initials: H
authors.lastname: Taguchi
authors.forename: Mariko
authors.initials: M
authors.lastname: Higa
authors.forename: Shigeki
authors.initials: S
authors.lastname: Hamada
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Matsui
authors.forename: Tadahiro
authors.initials: T
authors.lastname: Ozawa
authors.forename: Shigeki
authors.initials: S
authors.lastname: Jin
authors.forename: Jun
authors.initials: J
authors.lastname: Watanabe
authors.forename: Jun
authors.initials: J
authors.lastname: Wasaki
authors.forename: Susumu
authors.initials: S
authors.lastname: Ito
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001441:Bacteroides fragilis / Q000201:enzymology* / Q000235:genetics; D002384:Catalysis; D004187:Disaccharides / Q000378:metabolism*; D005798:Genes, Bacterial; D005947:Glucose / Q000378:metabolism*; D008351:Mannans / Q000378:metabolism*; D008969:Molecular Sequence Data; D006005:Phosphorylases / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D004187:Disaccharides; D008351:Mannans; D006005:Phosphorylases; D005947:Glucose