title: Dynamic fluorescence imaging for multiparametric measurement of tumor vasculature.
abstract: Angiogenesis is essential for tumor growth and a promising target for cancer therapy. Blood vessel monitoring is an indispensable tool for evaluation and development of anti-angiogenic drugs. Here, we report a new noninvasive in vivo imaging tool, named dynamic fluorescence imaging (DyFI), for the simultaneous measurement of multiple vascular parameters including vascular density, perfusion rate, and permeability using spatiotemporal profiles of indocyanine green. Using DyFI in a tumor xenograft model, we quantitatively measured multiple vascular parameters in tumors and normal tissues with high spatial resolution. The multimodality of this method allowed us to find negative spatial correlations between perfusion and permeability. Moreover, DyFI was effective for revealing the early effects of an anti-angiogenic drug. We suggest that DyFI could be a useful tool for the preclinical development of anti-angiogenic drugs.
authors.affiliation: Department of Bio and Brain Engineering, Cell Signaling and Bioimaging Laboratory, KAIST, 335 Gwahangno, Yuseong-gu, Daejeon 305-701, South Korea.
authors.forename: Myunghwan
authors.initials: M
authors.lastname: Choi
authors.forename: Kyungsun
authors.initials: K
authors.lastname: Choi
authors.forename: Seung-Wook
authors.initials: SW
authors.lastname: Ryu
authors.forename: Jungwhoi
authors.initials: J
authors.lastname: Lee
authors.forename: Chulhee
authors.initials: C
authors.lastname: Choi
mesh_terms: D020533:Angiogenesis Inhibitors / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D045744:Cell Line, Tumor; D003952:Diagnostic Imaging / Q000379:methods*; D007091:Image Processing, Computer-Assisted; D007208:Indocyanine Green / Q000737:chemistry / Q000378:metabolism; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008954:Models, Biological; D009369:Neoplasms / Q000098:blood supply* / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D009389:Neovascularization, Pathologic / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D012044:Regression Analysis; D013050:Spectrometry, Fluorescence / Q000379:methods*; D018709:Statistics, Nonparametric; D014183:Transplantation, Heterologous
chemical_list: D020533:Angiogenesis Inhibitors; D007208:Indocyanine Green