title: Design and evaluation of a recombinant multi-epitope-based ELISA for the serological surveillance of HEV infection in northern China.
abstract: In this study, seven recombinant epitope peptides from within the ORF2 protein of the local genotype 4 swine hepatitis E virus (HEV) DQ strain were designed and analyzed. Then, a new multi-epitope-based ELISA was established. In comparison with a commercial kit, this test exhibited good specificity and sensitivity for anti-HEV genotype 4. Subsequently, this test was applied for analyzing serum samples from either swine herds or human populations in northern China. The overall seroprevalence rate of anti-HEV IgG reached up to 40.4% for swine and 8.1% for humans. A statistical difference was observed for humans in rural and urban areas, with a higher prevalence for people living in rural than urban areas. Moreover, sequencing confirmed that all RNA-positive samples belonged to genotype 4.
authors.affiliation: Department of Animal Husbandry & Veterinary Medicine, Liaoning Medical University, Renmin Street 5-48, Jinzhou 121001, China.
authors.forename: Gao
authors.initials: G
authors.lastname: Shenyang
authors.forename: Li
authors.initials: L
authors.lastname: Dandan
authors.forename: Fan
authors.initials: F
authors.lastname: Chen
authors.forename: Wu
authors.initials: W
authors.lastname: Shuliang
authors.forename: Zhou
authors.initials: Z
authors.lastname: Tiezhong
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000595:Amino Acid Sequence; D000818:Animals; D002648:Child; D002681:China / Q000453:epidemiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D000939:Epitopes; D005838:Genotype; D016751:Hepatitis E / Q000097:blood / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D016752:Hepatitis E virus / Q000302:isolation & purification*; D006801:Humans; D008875:Middle Aged; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012680:Sensitivity and Specificity; D016036:Seroepidemiologic Studies; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D055815:Young Adult
chemical_list: D000939:Epitopes; D014764:Viral Proteins