title: Anthocyanin accumulation and expression of anthocyanin biosynthetic genes in radish (Raphanus sativus).
abstract: Radish [Raphanus sativus (Rs)] is an important dietary vegetable in Asian countries, especially China, Japan, and Korea. To elucidate the molecular mechanisms of anthocyanin accumulation in radish, the gene expression of enzymes directly involved in anthocyanin biosynthesis was analyzed. These genes include phenylalanine ammonia lyase (PAL), cinnamate 4-hydroxylase (C4H), 4-coumarate-CoA ligase (4CL), chalcone synthase (CHS), chalcone isomerase (CHI), flavanone 3-hydroxylase (F3H), dihydroflavonol reductase (DFR), and anthocyanidin synthase (ANS). RsDFR and RsANS were found to accumulate in the flesh or skin of two radish cultivars (Man Tang Hong and Hong Feng No.1). Radish skin contained higher CHS, CHI, and F3H transcript levels than radish flesh in all three cultivars. In the red radish, 16 anthocyanins were separated and identified by high-performance liquid chromatography (HPLC) and elctrospray ionization-tandem mass spectrometry (ESI-MS/MS). Some of them were acylated with coumaroyl, malonoyl, feruoyl, and caffeoyl moieties. Furthermore (-)-epicatechin and ferulic acid were also identified in the three cultivars.
authors.affiliation: Department of Crop Science, College of Agriculture and Life Sciences, Chungnam National University, 79 Daehangno, Yuseong-gu, Daejeon, 305-764, Korea.
authors.forename: Nam Il
authors.initials: NI
authors.lastname: Park
authors.forename: Hui
authors.initials: H
authors.lastname: Xu
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Li
authors.forename: In Hyuk
authors.initials: IH
authors.lastname: Jang
authors.forename: Suhyoung
authors.initials: S
authors.lastname: Park
authors.forename: Gil Hwan
authors.initials: GH
authors.lastname: Ahn
authors.forename: Yong Pyo
authors.initials: YP
authors.lastname: Lim
authors.forename: Sun Ju
authors.initials: SJ
authors.lastname: Kim
authors.forename: Sang Un
authors.initials: SU
authors.lastname: Park
mesh_terms: D000872:Anthocyanins / Q000096:biosynthesis*; D053898:Biosynthetic Pathways; D018506:Gene Expression Regulation, Plant; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D031224:Raphanus / Q000235:genetics* / Q000378:metabolism*
chemical_list: D000872:Anthocyanins; D010940:Plant Proteins