title: Antiproliferative and antioxidant activities of Juglans regia fruit extracts.
abstract: BACKGROUND
  Cancer chemopreventive action of walnut [Juglans regia L. (Juglandaceae)] has been explored.
  
  
  OBJECTIVE
  This study evaluated antiproliferative and antioxidant activities of walnut.
  
  
  METHODS
  Various fractions of walnut extract have been screened for antiproliferative activity against human cancer cell lines using the MTT assay. All these fractions have also been evaluated for total phenolic content, antioxidant activity, and reducing power capacity.
  
  
  CONCLUSIONS
  Chloroform and ethyl acetate fractions exhibited a high level of antiproliferation against HepG-2, liver cancer cell line (IC(50) = 9 and 15 µg/mL, respectively).
  
  
  CONCLUSIONS
  Exhibiting high phenolic content, antioxidant activity, and potent antiproliferative activity, walnut may act as a cancer chemopreventive agent.
authors.affiliation: Analytical Chemistry Department, Central Institute of Medicinal and Aromatic Plants (CIMAP-CSIR), Lucknow, Uttar Pradesh, India.
authors.forename: Arvind Singh
authors.initials: AS
authors.lastname: Negi
authors.forename: Suaib
authors.initials: S
authors.lastname: Luqman
authors.forename: Suchita
authors.initials: S
authors.lastname: Srivastava
authors.forename: Vinay
authors.initials: V
authors.lastname: Krishna
authors.forename: Namita
authors.initials: N
authors.lastname: Gupta
authors.forename: Mahendra Pandurang
authors.initials: MP
authors.lastname: Darokar
mesh_terms: D000085:Acetates / Q000737:chemistry; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D002725:Chloroform / Q000737:chemistry; D004354:Drug Screening Assays, Antitumor / Q000379:methods; D004610:Ellagic Acid / Q000737:chemistry; D005638:Fruit / Q000737:chemistry*; D005707:Gallic Acid / Q000737:chemistry; D056945:Hep G2 Cells; D006801:Humans; D020128:Inhibitory Concentration 50; D031324:Juglans / Q000737:chemistry*; D010084:Oxidation-Reduction / Q000187:drug effects; D010636:Phenols / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000085:Acetates; D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D010636:Phenols; D010936:Plant Extracts; D004610:Ellagic Acid; D005707:Gallic Acid; C007650:ethyl acetate; D002725:Chloroform