title: Intracellular pH distribution as a cell health indicator in Saccharomyces cerevisiae.
abstract: Internal pH regulation is vital for many cell functions, including transport mechanisms and metabolic enzyme activity. More specifically, transport mechanisms are to a wide degree governed by internal pH distributions. We introduce the term standard deviation of the intracellular pH (s.d.(pH(int))) to describe the internal pH distributions. The cellular pH distributional response to external stress such as heat has not previously been determined. In this study, the intracellular pH (pH(i)) and the s.d.(pH(int)) of Saccharomyces cerevisiae cells exposed to supralethal temperatures were measured using fluorescence ratio imaging microscopy (FRIM). An exponential decline in pH(i) was observed after an initial small decline. For the first time, we report the use of FRIM for determining in vivo plasma membrane proton permeability coefficients in yeast. Furthermore, the exponential decay of pH(i) and the rupture of the cell plasma membrane, as measured by propidium iodide staining, at 70°C were not simultaneous but were separated by a significant temporal difference. Finally, a nonlinear relationship between the pH(i) and s.d.(pH(int)) was found; i.e. the s.d.(pH(int)) was significantly more sensitive to supralethal temperatures than pH(i). s.d.(pH(int)) is therefore proposed as an early health/vitality indicator in S. cerevisiae cells exposed to heat stress.
authors.affiliation: Department of Food Science, University of Copenhagen, , Frederiksberg, Denmark. taa@life.ku.dk
authors.forename: Thomas
authors.initials: T
authors.lastname: Aabo
authors.forename: Jesper
authors.initials: J
authors.lastname: Glückstad
authors.forename: Henrik
authors.initials: H
authors.lastname: Siegumfeldt
authors.forename: Nils
authors.initials: N
authors.lastname: Arneborg
mesh_terms: D000136:Acid-Base Equilibrium / Q000502:physiology*; D002463:Cell Membrane Permeability / Q000502:physiology*; D005452:Fluoresceins; D006304:Health Status; D006863:Hydrogen-Ion Concentration; D007424:Intracellular Fluid / Q000737:chemistry*; D008856:Microscopy, Fluorescence / Q000379:methods; D011419:Propidium; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D013388:Succinimides; D013696:Temperature; D013997:Time Factors
chemical_list: C087165:5-(6)-carboxyfluorescein diacetate succinimidyl ester; D005452:Fluoresceins; D013388:Succinimides; D011419:Propidium