title: Evaluation of the potential risk of porcine endogenous retrovirus (PERV) infection in nude mice.
abstract: Nude mice (BALB/c) were grafted with human 293 cells and PERV (porcine endogenous retrovirus)-IRES-EGFP (a packageable retroviral vector plasmid containing an internal ribosome entry site-enhanced green fluorescent protein)-producing pig PK15 cells in order to determine whether the pig cells could transmit PERV-IRES-EGFP to mice and human 293 cells in vivo. None of the transplanted human 293 cell lines were infected by PERV, but PCR analysis identified PERV-B provirus integration into both the heart and salivary gland of the inoculated nude mice. Our data indicate that hearts and salivary glands can be used to identify PERV-B receptors.
authors.affiliation: Department of Microbiology and Institute of Basic Science, Dankook University, Cheonan 330-714, Korea.
authors.forename: Eun Hye
authors.initials: EH
authors.lastname: Bae
authors.forename: Yong-Tae
authors.initials: YT
authors.lastname: Jung
mesh_terms: D000818:Animals; D002460:Cell Line; D004195:Disease Models, Animal; D020077:Endogenous Retroviruses / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D017988:Gammaretrovirus / Q000235:genetics / Q000502:physiology*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D012192:Retroviridae Infections / Q000635:transmission* / Q000662:veterinary* / Q000821:virology; D013552:Swine; D013553:Swine Diseases / Q000635:transmission / Q000821:virology*; D014183:Transplantation, Heterologous