title: Diversity analysis of Burkholderia cepacia complex in the water bodies of West Lake, Hangzhou, China.
abstract: A survey of Burkholderia cepacia complex (Bcc) species was conducted in water bodies of West Lake in China. A total of 670 bacterial isolates were recovered on selective media. Out of them, 39.6% (265 isolates) were assigned to the following species: Burkholderia multivorans, Burkholderia cenocepacia recA lineage IIIA, IIIB, Burkholderia stabilis, Burkholderia vietnamiensis, and Burkholderia seminalis while B. cenocepacia is documented as a dominant Bcc species in water of West Lake. In addition, all Bcc isolates tested were PCR negative for the cblA and esmR transmissibility marker genes except B. cenocepacia IIIB A8 which was positive for esmR genelater. The present study raises great concerns on the role of West Lake as a "reservoir" for potential Bcc pathogenic strains.
authors.affiliation: State Key Laboratory of Rice Biology and Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture, Institute of Biotechnology, Zhejiang University, Hangzhou 310029, P R China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Fang
authors.forename: Guan-Lin
authors.initials: GL
authors.lastname: Xie
authors.forename: Miao-Miao
authors.initials: MM
authors.lastname: Lou
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Muhammad
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D042602:Burkholderia cepacia complex / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D002681:China; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005618:Fresh Water / Q000382:microbiology*; D008969:Molecular Sequence Data; D058889:Molecular Typing; D012150:Polymorphism, Restriction Fragment Length; D011938:Rec A Recombinases; D017422:Sequence Analysis, DNA
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D011938:Rec A Recombinases