title: Aurora-C interacts with and phosphorylates the transforming acidic coiled-coil 1 protein.
abstract: Aurora-C, a member of the Aurora kinase family, is implicated in the regulation of mitosis. In contrast to Aurora-A and Aurora-B its cellular localization and functions are poorly characterized. TACC1 protein belongs to the transforming acidic coiled-coil family shown to interact with the Aurora kinases. In the present study we analyzed the interaction between Aurora-C and TACC1 by means of immunofluorescence (IF), co-immunoprecipitation (IP) and in vitro phosphorylation experiments. We demonstrated that Aurora-C and TACC1 proteins co-localize to the midbody of HeLa cells during cytokinesis. Immunoprecipitated TACC1 from HeLa cell extracts was associated with Aurora-C. In addition, the interaction of the two proteins was tested by analyzing the phosphorylation of TACC1 in vitro. The results demonstrated that TACC1 is phosphorylated by Aurora-C on a serine at position 228. In conclusion, the study demonstrated that TACC1 localizes at the midbody during cytokinesis and interacts with and is a substrate of Aurora-C, which warrant further investigation in order to elucidate the functional significance of this interaction.
authors.affiliation: CNRS-UMR 6061, Institut Génétique et Développement, IFR 140, UEB-Université Rennes 1, 2 Avenue du Pr Léon Bernard, Rennes Cedex, France.
authors.forename: Jean-Charles
authors.initials: JC
authors.lastname: Gabillard
authors.forename: Salvatore
authors.initials: S
authors.lastname: Ulisse
authors.forename: Enke
authors.initials: E
authors.lastname: Baldini
authors.forename: Salvatore
authors.initials: S
authors.lastname: Sorrenti
authors.forename: Jean-Yves
authors.initials: JY
authors.lastname: Cremet
authors.forename: Carmela
authors.initials: C
authors.lastname: Coccaro
authors.forename: Claude
authors.initials: C
authors.lastname: Prigent
authors.forename: Massimino
authors.initials: M
authors.lastname: D'Armiento
authors.forename: Yannick
authors.initials: Y
authors.lastname: Arlot-Bonnemains
mesh_terms: D064107:Aurora Kinase B; D064108:Aurora Kinase C; D064127:Aurora Kinases; D048749:Cytokinesis; D005326:Fetal Proteins / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D047468:Immunoprecipitation; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D012694:Serine / Q000235:genetics / Q000378:metabolism
chemical_list: D005326:Fetal Proteins; D008869:Microtubule-Associated Proteins; D009687:Nuclear Proteins; C120584:TACC1 protein, human; D012694:Serine; C576921:AURKB protein, human; C576927:AURKC protein, human; D064107:Aurora Kinase B; D064108:Aurora Kinase C; D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases