title: Biochemical properties of pancreatic colipase from the common stingray Dasyatis pastinaca.
abstract: BACKGROUND
  Pancreatic colipase is a required co-factor for pancreatic lipase, being necessary for its activity during hydrolysis of dietary triglycerides in the presence of bile salts. In the intestine, colipase is cleaved from a precursor molecule, procolipase, through the action of trypsin. This cleavage yields a peptide called enterostatin knoswn, being produced in equimolar proportions to colipase.
  
  
  RESULTS
  In this study, colipase from the common stingray Dasyatis pastinaca (CoSPL) was purified to homogeneity. The purified colipase is not glycosylated and has an apparent molecular mass of around 10 kDa. The NH2-terminal sequencing of purified CoSPL exhibits more than 55% identity with those of mammalian, bird or marine colipases. CoSPL was found to be less effective activator of bird and mammal pancreatic lipases than for the lipase from the same specie. The apparent dissociation constant (Kd) of the colipase/lipase complex and the apparent Vmax of the colipase-activated lipase values were deduced from the linear curves of the Scatchard plots. We concluded that Stingray Pancreatic Lipase (SPL) has higher ability to interact with colipase from the same species than with the mammal or bird ones.
  
  
  CONCLUSIONS
  The fact that colipase is a universal lipase cofactor might thus be explained by a conservation of the colipase-lipase interaction site. The results obtained in the study may improve our knowledge of marine lipase/colipase.
authors.affiliation: Laboratoire de Biochimie et de Génie Enzymatique des Lipases, ENIS route de Soukra, BP1173, University of Sfax - 3038 Sfax, Tunisia.
authors.forename: Abir
authors.initials: A
authors.lastname: Ben Bacha
authors.forename: Aida
authors.initials: A
authors.lastname: Karray
authors.forename: Lobna
authors.initials: L
authors.lastname: Daoud
authors.forename: Emna
authors.initials: E
authors.lastname: Bouchaala
authors.forename: Madiha
authors.initials: M
authors.lastname: Bou Ali
authors.forename: Youssef
authors.initials: Y
authors.lastname: Gargouri
authors.forename: Yassine
authors.initials: Y
authors.lastname: Ben Ali
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003089:Colipases / Q000737:chemistry* / Q000302:isolation & purification; D006801:Humans; D007700:Kinetics; D008049:Lipase / Q000378:metabolism; D008969:Molecular Sequence Data; D010179:Pancreas / Q000201:enzymology*; D016415:Sequence Alignment; D012860:Skates, Fish; D013045:Species Specificity; D014280:Triglycerides / Q000378:metabolism
chemical_list: D003089:Colipases; D014280:Triglycerides; D008049:Lipase; C005830:tributyrin