title: [Expression of porcine interleukin-18 in baculovirus/insect cells].
abstract: IL-18, as a polyphonic cytokine, is important in immune response and physiologic function. We designed one pair of primers, amplified the porcine IL-18 gene fused with a C-terminal 6xHistidine tag, and then subcloned into the pFastBacDual of Baculovirus transfer vector and transformed into DH10Bac containing a shuttle vector of Bacmid. After co-transfecting the recombinant plasmid into insect cells, the 18 kDa expressed protein of porcine IL-18 was detected by SDS-PAGE; the specificity of expressed protein was confirmed by Western blotting. The purified porcine IL-18 protein induced obvious proliferation of porcine T lymphocytes in vitro, which indicated that the expression of IL-18 had high biological activity.
authors.affiliation: College of Animal Husbandry and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China.
authors.forename: Zhenya
authors.initials: Z
authors.lastname: Wang
authors.forename: Yanbin
authors.initials: Y
authors.lastname: Wang
authors.forename: Hongying
authors.initials: H
authors.lastname: Chen
authors.forename: Panfeng
authors.initials: P
authors.lastname: Shao
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Ning
authors.forename: Na
authors.initials: N
authors.lastname: Pan
authors.forename: Lijing
authors.initials: L
authors.lastname: Han
authors.forename: Baoan
authors.initials: B
authors.lastname: Cui
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D005822:Genetic Vectors / Q000235:genetics*; D007313:Insecta / Q000235:genetics / Q000378:metabolism*; D020382:Interleukin-18 / Q000096:biosynthesis* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D013552:Swine; D014162:Transfection
chemical_list: D020382:Interleukin-18; D011994:Recombinant Proteins