title: Multi-day pre-clinical demonstration of glucose/galactose binding protein-based fiber optic sensor.
abstract: We report here the first pre-clinical demonstration of continuous glucose tracking by fluorophore-labeled and genetically engineered glucose/galactose binding protein (GGBP). Acrylodan-labeled GGBP was immobilized in a hydrogel matrix at the tip of a small diameter optical fiber contained in a stainless steel needle. The fiber optic biosensors were inserted subcutaneously into Yucatan and Yorkshire swine, and the sensor response to changing glucose levels was monitored at intervals over a 7-day period. Sensor mean percent error on day 7 was 16.4±5.0% using a single daily reference blood glucose value to calibrate the sensor. The GGBP sensor's susceptibility to common interferents was tested in a well-plate system using human sera. No significant interference was observed from the tested interferents except for tetracycline at the drug's maximum plasma concentration. The robust performance of the GGBP-based fiber optic sensor in swine models and resistance to interferents indicates the potential of this technology for continuous glucose monitoring in humans.
authors.affiliation: BD Technologies, 21 Davis Drive, Research Triangle Park, NC 27709, USA.
authors.forename: Kristin
authors.initials: K
authors.lastname: Weidemaier
authors.forename: Alexander
authors.initials: A
authors.lastname: Lastovich
authors.forename: Steven
authors.initials: S
authors.lastname: Keith
authors.forename: J Bruce
authors.initials: JB
authors.lastname: Pitner
authors.forename: Mark
authors.initials: M
authors.lastname: Sistare
authors.forename: Ross
authors.initials: R
authors.lastname: Jacobson
authors.forename: David
authors.initials: D
authors.lastname: Kurisko
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D001786:Blood Glucose / Q000032:analysis*; D029968:Escherichia coli Proteins / Q000737:chemistry; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry; D023421:Models, Animal; D008991:Monitoring, Physiologic / Q000295:instrumentation / Q000379:methods; D009004:Monosaccharide Transport Proteins / Q000737:chemistry; D055100:Optical Fibers; D011487:Protein Conformation; D034421:Sus scrofa; D013552:Swine; D013556:Swine, Miniature
chemical_list: D001786:Blood Glucose; D029968:Escherichia coli Proteins; D055767:Immobilized Proteins; D009004:Monosaccharide Transport Proteins; C509064:mglB protein, E coli