title: [Estimation of early postmortem interval using beta-actin mRNA in rat's brain, heart and kidney].
abstract: OBJECTIVE
  To explore the relationship between beta-actin mRNA degradation in SD rat's brain, heart and kidney and early postmortem interval (PMI) in order to find new markers for estimating early PMI.
  
  
  METHODS
  Rats were sacrificed and kept in the place at a temperature of 20 degrees C. The total RNA were extracted from the brain, heart and kidney at different PMI points. Real time RT-PCR was applied to determine beta-actin mRNA levels in total RNA and the results were given in the form of Ct values. Linear relationships between PMI and Ct values were obtained and the functions of linear regression were established.
  
  
  RESULTS
  The great decrease of beta-actin mRNA level were observed in the three organs. The degradation rate was obviously higher in 24 hours after death in the heart and kidney. However, there were no significant changes in the brain. The changes of Ct values and PMI showed a good linear relationship.
  
  
  CONCLUSIONS
  beta-actin mRNA in rat's brain, heart and kidney degrades obviously after death and can be used for estimating early PMI by its degradation rules.
authors.affiliation: Department of Forensic Medicine, Shanghai Medical College of Fudan University, Shanghai 200032, China. 082101057@fudan.edu.cn
authors.forename: Yue-lin
authors.initials: YL
authors.lastname: Liu
authors.forename: Kai-Jun
authors.initials: KJ
authors.lastname: Ma
authors.forename: Wen-can
authors.initials: WC
authors.lastname: Li
authors.forename: Hong-mei
authors.initials: HM
authors.lastname: Xu
authors.forename: Ai-min
authors.initials: AM
authors.lastname: Xue
authors.forename: Yi-wen
authors.initials: YW
authors.lastname: Shen
authors.forename: Yue-qin
authors.initials: YQ
authors.lastname: Zhou
authors.forename: Zi-qin
authors.initials: ZQ
authors.lastname: Zhao
mesh_terms: D000199:Actins / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D005554:Forensic Medicine / Q000379:methods; D007668:Kidney / Q000378:metabolism*; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D011180:Postmortem Changes; D020871:RNA Stability; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013997:Time Factors
chemical_list: D000199:Actins; D012333:RNA, Messenger