title: miR-148a promoted cell proliferation by targeting p27 in gastric cancer cells.
abstract: Accumulating evidence has shown that miRNAs are aberrantly expressed in human gastric cancer and crucial to tumorigenesis. Herein, we identified the role of miR-148a in gastric cell proliferation. miR-148a knockdown inhibited cell proliferation in gastric cancer cell lines. Conversely, miR-148a overexpression promoted cell proliferation and cell cycle progression. p27, a key inhibitor of cell cycle, was verified as the target of miR-148a, indicating miR-148a might downregulate p27 expression to promote gastric cell proliferation. Moreover, we confirmed that miR-148a expression was frequently and dramatically downregulated in human advanced gastric cancer tissues, and observed a good inverse correlation between miR-148a and p27 expression in tumor samples. Thus, our results demonstrated that miR-148a downregulation might exert some sort of antagonistic function in cell proliferation, rather than promote cell proliferation in gastric cancer.
authors.affiliation: State Key Laboratory of Proteomics, Genetic Laboratory of Development and Diseases, Institute of Biotechnology, Beijing, PR China.
authors.forename: Shui-Long
authors.initials: SL
authors.lastname: Guo
authors.forename: Zheng
authors.initials: Z
authors.lastname: Peng
authors.forename: Xue
authors.initials: X
authors.lastname: Yang
authors.forename: Kai-Ji
authors.initials: KJ
authors.lastname: Fan
authors.forename: Hui
authors.initials: H
authors.lastname: Ye
authors.forename: Zhen-Hua
authors.initials: ZH
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Xu
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: You-Liang
authors.initials: YL
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Teng
authors.forename: Xiao
authors.initials: X
authors.lastname: Yang
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000378:metabolism*; D015536:Down-Regulation; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000378:metabolism*
chemical_list: C550570:MIRN148 microRNA, human; D035683:MicroRNAs; D050760:Cyclin-Dependent Kinase Inhibitor p27