title: Single mutation converts mild pathotype of the Pepino mosaic virus into necrotic one.
abstract: Pepino mosaic virus (PepMV) is a member of the Flexiviridae family, genus Potexvirus. PepMV isolates from the same genotype differ in the symptoms they induce in Datura inoxia and Solanum lycopersicum. Necrotic isolates cause necrosis on these plants whereas mild isolates are very often asymptomatic or induce mild mosaic. Sequence analysis of the genomes of mild (PepMV-P22) and necrotic (PepMV-P19) strains revealed that they differ by several nonsynonymous mutations. Eleven mutations are located in the RdRp coding region and one mutation is placed in the TGB3 coding region. To investigate whether these mutations affect the symptom induction, both PepMV-P22 and PepMV-P19 were mutagenized. As a result we found that the genetic determinant responsible for necrosis induction was amino acid 67 of TGB3. This showed that one point mutation is sufficient to alter the virus from mild to aggressive in tomato and D. inoxia.
authors.affiliation: Institute of Plant Protection-National Research Institute, Department of Virology and Bacteriology, ul. Władysława Węgorka 20, 60-318 Poznań, Poland. B.Hasiow@iorpib.poznan.pl
authors.forename: Beata
authors.initials: B
authors.lastname: Hasiów-Jaroszewska
authors.forename: Natasza
authors.initials: N
authors.lastname: Borodynko
authors.forename: Paulina
authors.initials: P
authors.lastname: Jackowiak
authors.forename: Marek
authors.initials: M
authors.lastname: Figlerowicz
authors.forename: Henryk
authors.initials: H
authors.lastname: Pospieszny
mesh_terms: D004252:DNA Mutational Analysis; D032302:Datura / Q000821:virology; D018551:Solanum lycopersicum / Q000821:virology; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000821:virology*; D017863:Potexvirus / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics*; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins; D037521:Virulence Factors