title: Pkc-dependent and pkc-independent cellular effects induced by recombinant transforming growth-factor type-Beta-1.
abstract: TGF-beta 1 purified from human platelets induces colony formation of rat fibroblasts reversibly when administered together with EGF. TGF-beta 1 has been reported by us to lead to stable transformation of rat fibroblasts, to activate latent Epstein-Barr virus, to exhibit tumor promoting activity for murine fibroblasts, and to cause the release of apoptosis-inducing signals from normal cells which are directed specifically against transformed cells. The use of recombinant TGF-beta 1 in this study showed that all the effects measured were indeed due to TGF-beta 1. In order to get information on intracellular signal pathways induced by TGF-beta 1, specific inhibitors of protein kinase C (PKC) were applied. Induction of EBV antigens by TGF-beta 1 was dependent on the function of PKC, whereas induction of apoptosis in transformed cells by TGF-beta 1-treated normal cells was independent of the action of PKC.
authors.affiliation: UNIV FREIBURG,INST MED MIKROBIOL & HYG,VIROL ABT,D-79104 FREIBURG,GERMANY. INST MOLEK MED & NAT STOFFORSCH,TUMORBIOL KLIN,FREIBURG,GERMANY.
authors.forename: S
authors.initials: S
authors.lastname: Strauss
authors.forename: M
authors.initials: M
authors.lastname: Weber
authors.forename: C
authors.initials: C
authors.lastname: Schachtele
authors.forename: G
authors.initials: G
authors.lastname: Bauer