title: Expression pattern of tumor endothelial marker 8 protein in gallbladder carcinomas.
abstract: Tumor endothelial marker 8 protein (TEM8) is highly specific to tumor angiogenesis and is not required for normal adult angiogenesis and hence might prove to be a target for anti-angiogenic therapies in the future. We here evaluated protein and gene expression patterns in human endothelial cells of benign gallbladder - gallstone diseases (GSDs) and gallbladder carcinomas (GBCs) using immunostaining, immunofluorescence and western blotting techniques. Subjects comprised 175 GBC patients, 38 males and 137 females, aged 30-85 years (mean age 50.3 ± 13.4 years) and twenty with GSDs, aged 30-75 years, (51.4 ± 10.0 years) for comparison (male 4/20 and females 16/20). TEM8 protein expression increased significantly (p < 0.0001) with increasing stage of GBC and was mostly limited to endothelial cells, although there was no significant change with the grade. Interestingly, only 80-85 kDa and 60 kDa isoforms of TEM8 increased significantly whereas 45 kDa isoform was absent in GBCs. Conclusions- These results suggest that TEM8 plays an unknown important biological role to promote tumor angiogenesis in GBC.
authors.affiliation: Surgical Oncology, Institute of Medical Science, Banaras Hindu University, Varanasi, India.
authors.forename: Sanjeev Kumar
authors.initials: SK
authors.lastname: Maurya
authors.forename: Mallika
authors.initials: M
authors.lastname: Tewari
authors.forename: Mohan
authors.initials: M
authors.lastname: Kumar
authors.forename: Mahendra Kumar
authors.initials: MK
authors.lastname: Thakur
authors.forename: Hari Shanker
authors.initials: HS
authors.lastname: Shukla
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism*; D015153:Blotting, Western; D016022:Case-Control Studies; D005260:Female; D005455:Fluorescent Antibody Technique; D005704:Gallbladder / Q000378:metabolism*; D005706:Gallbladder Neoplasms / Q000098:blood supply / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008840:Microfilament Proteins; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000378:metabolism*; D009367:Neoplasm Staging; D009389:Neovascularization, Pathologic / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*
chemical_list: C473287:ANTXR1 protein, human; D014408:Biomarkers, Tumor; D008840:Microfilament Proteins; D009363:Neoplasm Proteins; D011956:Receptors, Cell Surface