title: Detection of KI polyomavirus and WU polyomavirus DNA by real-time polymerase chain reaction in nasopharyngeal swabs and in normal lung and lung adenocarcinoma tissues.
abstract: Polyomaviruses KI (KIPyV) and WU (WUPyV) were detected from 7 (3.0%) and 38 (16.4%) of 232 children with respiratory tract infections by real-time PCR. The rates of infection by KIPyV and WUPyV alone were 3 of 7 (42.9%) and 20 of 38 (52.6%), respectively. In the other samples, various viruses (human respiratory syncytial virus, human metapneumovirus, human rhinovirus, parainfluenza virus 1 and human bocavirus) were detected simultaneously. One case was positive for KIPyV, WUPyV and hMPV. There was no obvious difference in clinical symptoms between KIPyV-positive and WUPyV-positive patients with or without coinfection. KIPyV was detected in one of 30 specimens of lung tissue (3.3%). Neither of the viruses was detected in 30 samples of lung adenocarcinoma tissue.
authors.affiliation: Department of Pediatrics, Hokkaido University Graduate School of Medicine, Sapporo, Japan.
authors.forename: Shinobu
authors.initials: S
authors.lastname: Teramoto
authors.forename: Miki
authors.initials: M
authors.lastname: Kaiho
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Takano
authors.forename: Rika
authors.initials: R
authors.lastname: Endo
authors.forename: Hideaki
authors.initials: H
authors.lastname: Kikuta
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Sawa
authors.forename: Tadashi
authors.initials: T
authors.lastname: Ariga
authors.forename: Nobuhisa
authors.initials: N
authors.lastname: Ishiguro
mesh_terms: D000230:Adenocarcinoma / Q000150:complications / Q000821:virology; D000077192:Adenocarcinoma of Lung; D000368:Aged; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D008168:Lung / Q000821:virology*; D008175:Lung Neoplasms / Q000150:complications / Q000821:virology; D008297:Male; D008875:Middle Aged; D009305:Nasopharynx / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011120:Polyomavirus / Q000235:genetics / Q000302:isolation & purification*; D027601:Polyomavirus Infections / Q000453:epidemiology* / Q000821:virology; D015995:Prevalence; D012141:Respiratory Tract Infections / Q000453:epidemiology* / Q000821:virology