title: Optimization of Inulinase Production from Garlic by Streptomyces sp. in Solid State Fermentation Using Statistical Designs.
abstract: Plackett-Burman design was employed for screening 18 nutrient components for the production of inulinase using Garlic as substrate by Streptomyces sp. in solid-state fermentation (SSF). From the experiments, 4 nutrients, namely, NH(4)NO(3), MnSO(4)·7H(2)O, Soya bean cake, and K(2)HPO(4) were found to be most significant nutrient components. Hence, these 4 components are selected. The selected components were optimized using response surface methodology (RSM). The optimum conditions are NH(4)NO(3)-6.63 mg/gds, MnSO(4)·7H(2)O-26.16 mg/gds, Soya bean cake-60.6 mg/gds, and K(2)HPO(4)-5.24 mg/gds. Under these conditions, the production of inulinase was found to be 76 U/gds.
authors.affiliation: Department of Chemical Engineering, Annamalai University, Annamalainagar, Tamilnadu 608002, India.
authors.forename: M
authors.initials: M
authors.lastname: Dilipkumar
authors.forename: M
authors.initials: M
authors.lastname: Rajasimman
authors.forename: N
authors.initials: N
authors.lastname: Rajamohan