title: Molecular cloning, sequence analysis and structure modeling of OmpR, the response regulator of Aeromonas hydrophila.
abstract: The ability of bacteria to survive and proliferate in changing environmental conditions, and during host cell invasion is the key to their pathogenicity. In order to achieve this, the bacteria use a signal transduction system, the two component regulatory system, which consists of a sensor kinase and a response regulator. The EnvZ/OmpR system regulates the porin genes ompF/ompC in response to changes in osmolarity. In the present study, the ompR gene of Aeromonas hydrophila (isolate Ah17) was cloned, sequenced and characterized. Further an attempt was made to analyze the structural characteristics of the OmpR protein from Aeromonas hydrophila. The three dimensional structure of the protein was predicted by homology modeling and the modeled structure was compared to other members of two component response regulators. This study would be helpful for structure based drug design approaches to generate drugs against this harmful pathogen to control its proliferation in both human and fish hosts.
authors.affiliation: Gene Regulation Laboratory, School of Biotechnology, Jawaharlal Nehru University, New Delhi, 110067, India.
authors.forename: Gagan
authors.initials: G
authors.lastname: Chhabra
authors.forename: Tanuja
authors.initials: T
authors.lastname: Upadhyaya
authors.forename: Aparna
authors.initials: A
authors.lastname: Dixit
mesh_terms: D016980:Aeromonas hydrophila / Q000235:genetics*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D019295:Computational Biology; D017931:DNA Primers / Q000235:genetics; D008957:Models, Genetic; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D015534:Trans-Activators / Q000737:chemistry* / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; D015534:Trans-Activators; C112177:osmolarity response regulator proteins