title: Internal distribution of uranium and associated genotoxic damages in the chronically exposed bivalve Corbicula fluminea.
abstract: Uranium (U) internal distribution and involved effects in the bivalve Corbicula fluminea have been studied after direct chronic exposure (90 d, 10 μg.L-1). U distribution was assessed at the subcellular level (Metal Rich Granules -MRG-, pellets and cytosol fractions) in two main organs of the bivalve (gills and visceral mass). Micro-localisation was investigated by TEM-EDX analysis in the gills epithelium. DNA damage in gill and hemolymph samples was measured by the Comet assay. The 90-d exposure period led to a significant increase of U concentration in gills over time (× 5) and a large U quantity in subcellular granules in gills. Finally, a significant increase (× 2) in DNA damage was noted in exposed gills and haemocytes. This study shows that the accumulation levels and consequently the potential toxicity cannot be successfully predicted only on the basis of concentration in water or in tissues and subcellular fractions after chronic exposure.
authors.affiliation: Laboratoire de Radioécologie et Ecotoxicologie, Institut de Radioprotection et de Sûreté Nucléaire, Cadarache, Bât, Saint Paul-Lez-Durance Cedex, France. olivier.simon@irsn.fr
authors.forename: Olivier
authors.initials: O
authors.lastname: Simon
authors.forename: Magali
authors.initials: M
authors.lastname: Floriani
authors.forename: Isabelle
authors.initials: I
authors.lastname: Cavalie
authors.forename: Virginie
authors.initials: V
authors.lastname: Camilleri
authors.forename: Christelle
authors.initials: C
authors.lastname: Adam
authors.forename: Rodolphe
authors.initials: R
authors.lastname: Gilbin
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Garnier-Laplace
mesh_terms: D000818:Animals; D020552:Comet Assay / Q000662:veterinary; D049874:Corbicula / Q000187:drug effects* / Q000235:genetics*; D004249:DNA Damage / Q000528:radiation effects*; D004781:Environmental Exposure; D005880:Gills / Q000737:chemistry / Q000528:radiation effects; D006458:Hemolymph / Q000737:chemistry / Q000528:radiation effects; D017348:Microscopy, Electron, Scanning Transmission / Q000662:veterinary; D011834:Radiation Monitoring; D014501:Uranium / Q000032:analysis* / Q000097:blood / Q000633:toxicity*; D014875:Water Pollutants, Radioactive / Q000032:analysis / Q000097:blood / Q000633:toxicity
chemical_list: D014875:Water Pollutants, Radioactive; D014501:Uranium