title: Electrostatic-assembly-driven formation of supramolecular rhombus microparticles and their application for fluorescent nucleic acid detection.
abstract: In this paper, we report on the large-scale formation of supramolecular rhombus microparticles (SRMs) driven by electrostatic assembly, carried out by direct mixing of an aqueous HAuCl(4) solution and an ethanol solution of 4,4'-bipyridine at room temperature. We further demonstrate their use as an effective fluorescent sensing platform for nucleic acid detection with a high selectivity down to single-base mismatch. The general concept used in this approach is based on adsorption of the fluorescently labeled single-stranded DNA (ssDNA) probe by SRM, which is accompanied by substantial fluorescence quenching. In the following assay, specific hybridization with its target to form double-stranded DNA (dsDNA) results in desorption of ssDNA from SRM surface and subsequent fluorescence recovery.
authors.affiliation: State Key Lab of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, China.
authors.forename: Hailong
authors.initials: H
authors.lastname: Li
authors.forename: Junfeng
authors.initials: J
authors.lastname: Zhai
authors.forename: Xuping
authors.initials: X
authors.lastname: Sun
mesh_terms: D020137:Base Pair Mismatch; D011232:Chemical Precipitation; D004247:DNA / Q000032:analysis; D005453:Fluorescence; D007700:Kinetics; D008863:Microspheres; D009693:Nucleic Acid Hybridization / Q000379:methods*; D009696:Nucleic Acids / Q000032:analysis*; D055672:Static Electricity
chemical_list: D009696:Nucleic Acids; D004247:DNA