title: Cell tracking in live Caenorhabditis elegans embryos via third harmonic generation imaging microscopy measurements.
abstract: In this study, we demonstrate the potential of employing third harmonic generation (THG) imaging microscopy measurements for cell tracking studies in live Caenorhabditis elegans (C. elegans) embryos. A 1028-nm femtosecond laser was used for the excitation of unstained C. elegans samples. Different C. elegans embryonic stages (from two-cell to threefold) were imaged. Live biological specimens were irradiated for prolonged periods of time (up to 7 h), testifying to the nondestructive nature of this nonlinear imaging technique. Thus, THG image contrast modality is a powerful diagnostic tool for probing in vivo cell division during early embryogenesis.
authors.affiliation: Foundation of Research and Technology-Hellas, Institute of Electronic Structure and Laser, PO Box 1385, Heraklion, Crete 71110, Greece.
authors.forename: George J
authors.initials: GJ
authors.lastname: Tserevelakis
authors.forename: George
authors.initials: G
authors.lastname: Filippidis
authors.forename: Evgenia V
authors.initials: EV
authors.lastname: Megalou
authors.forename: Costas
authors.initials: C
authors.lastname: Fotakis
authors.forename: Nektarios
authors.initials: N
authors.lastname: Tavernarakis
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000166:cytology* / Q000196:embryology; D058948:Cell Tracking / Q000379:methods*; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000196:embryology; D007834:Lasers; D008853:Microscopy / Q000379:methods*; D008954:Models, Biological; D017711:Nonlinear Dynamics; D013997:Time Factors; D059008:Time-Lapse Imaging / Q000379:methods*