title: In-vitro cytotoxicity and cell uptake study of gelatin-coated magnetic iron oxide nanoparticles.
abstract: The aim of this study was to modify the surfaces of magnetic iron oxide nanoparticles (IOPs) with gelatin in order to reduce cytotoxicity and enhance cellular uptake. The gelatin-coated IOPs were characterized in terms of their functionalization, size, surface charge, morphology and crystalline structure using Fourier transform infrared (FT-IR) spectroscopy, thermogravimetric analysis (TGA), dynamic light scattering (DLS), transmission electron microscopy (BIO-TEM) and x-ray diffraction (XRD) analysis. The cytotoxicity of the gelatin-coated IOPs to human fibroblasts was assessed using an MTT-assay and was compared with uncoated IOPs. Similarly, the cellular uptake of the coated and uncoated IOPs was visualized using BIO-TEM and quantified using inductively coupled plasma spectroscopy (ICPS). As shown by the Fourier emission scanning electron microscopy (FE-SEM) and viability test, the massive uptake of uncoated IOPs lead to reduced viability. However, gelatin coating lead to increased viability and slow uptake without any visible distortion to the cell morphology.
authors.affiliation: Department of Bionanosystem Engineering, Chonbuk National University, Chonju, Republic of Korea. babita@uow.edu.au
authors.forename: Babita
authors.initials: B
authors.lastname: Gaihre
authors.forename: Young
authors.initials: Y
authors.lastname: Hee Lee
authors.forename: Myung Seob
authors.initials: MS
authors.lastname: Khil
authors.forename: Ho Keun
authors.initials: HK
authors.lastname: Yi
authors.forename: Hak Yong
authors.initials: HY
authors.lastname: Kim
mesh_terms: D002470:Cell Survival; D002478:Cells, Cultured; D016503:Drug Delivery Systems / Q000379:methods; D005290:Ferric Compounds / Q000737:chemistry / Q000494:pharmacology*; D005780:Gelatin / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D053758:Nanoparticles; D010316:Particle Size
chemical_list: D005290:Ferric Compounds; C000499:ferric oxide; D005780:Gelatin