title: Lung immunoreactivity and airway inflammation: their assessment after scorpion envenomation.
abstract: Release and activation of pro-inflammatory mediators are among the most important induced factors that are involved in the scorpion envenomation pathogenesis. Inflammatory response and lung reactivity were studied in mice following subcutaneous injection with Androctonus australis hector (Aah) venom. Venom immunodetection in lungs and sequestered cell population in the airways were determined. Cytokines, cellular peroxidase activities (eosinophil peroxidase, myeloperoxydase), and IgE antibodies were also assessed. Immunohistochemical study revealed a positive detection of the Aah venom in the alveolar wall, venule lumens, and inside inflammatory cells. Severe lung edema associated with rapid inflammatory response was observed after animal envenomation. Lung neutrophilia and eosinophilia were accompanied with IL-4, IL-5 release, and IgE synthesis. In conclusion, high cytokine levels, recruitment of inflammatory cells (eosinophils and neutrophils), and increased IgE concentration may contribute to the exacerbation and maintenance of the induced inflammatory response in lungs by scorpion venom. These results lead to the better understanding of this induced pathogenesis and could help the physicians to take care of envenomed patients.
authors.affiliation: Laboratoire de Biologie Cellulaire et Moléculaire, Faculté des Sciences Biologiques, Université des Sciences et de la Technologie Houari Boumédienne, BP 32, El-Alia Bab Ezzouar, 16111, Algiers, Algeria.
authors.forename: Sonia
authors.initials: S
authors.lastname: Adi-Bessalem
authors.forename: Amina
authors.initials: A
authors.lastname: Mendil
authors.forename: Djelila
authors.initials: D
authors.lastname: Hammoudi-Triki
authors.forename: Fatima
authors.initials: F
authors.lastname: Laraba-Djebari
mesh_terms: D000818:Animals; D047209:Eosinophil Peroxidase / Q000096:biosynthesis / Q000378:metabolism; D004802:Eosinophilia / Q000276:immunology; D007073:Immunoglobulin E / Q000096:biosynthesis / Q000276:immunology; D007249:Inflammation / Q000276:immunology / Q000473:pathology; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D015848:Interleukin-5 / Q000096:biosynthesis; D008168:Lung / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D051379:Mice; D009195:Peroxidase / Q000378:metabolism; D011654:Pulmonary Edema / Q000276:immunology* / Q000473:pathology; D065008:Scorpion Stings / Q000276:immunology* / Q000473:pathology; D012604:Scorpion Venoms / Q000276:immunology / Q000378:metabolism / Q000506:poisoning*; D012605:Scorpions
chemical_list: D015848:Interleukin-5; D012604:Scorpion Venoms; D018799:Intercellular Adhesion Molecule-1; D015847:Interleukin-4; D007073:Immunoglobulin E; D047209:Eosinophil Peroxidase; D009195:Peroxidase