title: dsDNA-coated quantum dots.
abstract: Because of their unique spectral properties, quantum dots (QDs) have recently proved useful as fluorescent labels for biosensing probes. We developed a versatile QD label by modifying dsDNA with biotin and thiol groups at opposite ends and attaching it to quantum dots via a metal-thiol bond. These dsDNA-coated QDs fluorescently label their targets through biotin-streptavidin binding and show excellent histological results when used to detect biotin-labeled chromosome probes. The dsDNA coating also circumvented the common problems of aggregation and steric hindrance that occur with other QD probes.
authors.affiliation: College of Life Science, Jilin University, No. 2699 Qian Jin Da Jie Road, Changchun City, Jilin Province, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Xing
authors.forename: Huaibin
authors.initials: H
authors.lastname: Shen
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Liu
authors.forename: Yingda
authors.initials: Y
authors.lastname: Du
authors.forename: Zhenxiang
authors.initials: Z
authors.lastname: Yu
authors.forename: Xia
authors.initials: X
authors.lastname: Chen
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002875:Chromosomes; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism; D005737:Garlic / Q000166:cytology; D015335:Molecular Probes / Q000737:chemistry* / Q000378:metabolism; D018517:Plant Roots / Q000166:cytology; D010957:Plasmids / Q000235:genetics; D045663:Quantum Dots
chemical_list: D005456:Fluorescent Dyes; D015335:Molecular Probes; D004247:DNA