title: Simultaneous biochemical and topographical patterning on curved surfaces using biocompatible sacrificial molds.
abstract: A method for the simultaneous (bio)chemical and topographical patterning of enclosed structures in poly(dimethyl siloxane) (PDMS) is presented. The simultaneous chemical and topography transference uses a water-soluble chitosan sacrificial mold to impart a predefined pattern with micrometric accuracy to a PDMS replica. The method is compared to conventional soft-lithography techniques on planar surfaces. Its functionality is demonstrated by the transference of streptavidin directly to the surface of the three-dimensional PDMS structures as well as indirectly using streptavidin-loaded latex nanoparticles. The streptavidin immobilized on the PDMS is tested for bioactivity by coupling with fluorescently labeled biotin. This proves that the streptavidin is immobilized on the PDMS surface, not in the bulk of the polymer, and is therefore accessible for use as signaling/binding element in micro and bioengineering. The use of a biocompatible polymer and processes enables the technique to be used for the chemical patterning of tissue constructions.
authors.affiliation: Nanobioengineering group, Institute for Bioengineering of Catalonia, 08028 Barcelona, Spain. javier.fernandez@wyss.harvard.edu
authors.forename: Javier G
authors.initials: JG
authors.lastname: Fernandez
authors.forename: Josep
authors.initials: J
authors.lastname: Samitier
authors.forename: Christopher A
authors.initials: CA
authors.lastname: Mills
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D001710:Biotin / Q000378:metabolism; D048271:Chitosan / Q000737:chemistry; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D019809:Streptavidin / Q000378:metabolism; D013499:Surface Properties; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D004129:Dimethylpolysiloxanes; D001710:Biotin; D048271:Chitosan; D019809:Streptavidin