title: A fast and simple in situ PCR method to detect human papilloma virus infection in archival paraffin-embedded tissues.
abstract: We describe a simple and fast method for the detection and localization of low copy numbers of HPV DNA in formalin-fixed and paraffin-embedded archival tissues. We have developed a protocol for direct bl situ-PCR in order to demonstrate its convenience in rapid and reproducible assessment of HPV infection in unknown biopsies. The morphological aspect of the tissues has been maintained, despite the multiple steps of fixation, permeabilization and thermal cycling, and positivity has been detected only in virus target cells.
authors.affiliation: NATL INST CANC RES,DEPT EXPT ONCOL,I-16132 GENOA,ITALY. UNIV GENOA,DEPT CLIN & EXPT ONCOL,I-16132 GENOA,ITALY. PERKIN ELMER CORP,I-10044 PIANEZZA,TURIN,ITALY. OSPED GALLIERA,I-16128 GENOA,ITALY.
authors.forename: T
authors.initials: T
authors.lastname: Mancuso
authors.forename: S
authors.initials: S
authors.lastname: Crovella
authors.forename: S
authors.initials: S
authors.lastname: Casazza
authors.forename: M
authors.initials: M
authors.lastname: Pescarolo
authors.forename: L
authors.initials: L
authors.lastname: Clerico
authors.forename: P
authors.initials: P
authors.lastname: Russo
authors.forename: S
authors.initials: S
authors.lastname: Parodi