title: Development and characterization of transgenic mouse models for conditional gene knockout in the blood-brain and blood-CSF barriers.
abstract: For many CNS acting drugs, penetration into the central nervous system (CNS) is limited by the blood-CNS-barriers. In an effort to quantitate the role of the protein components that make up the blood-CNS-barriers, we created transgenic mice that allow conditional gene knockout using Cre/loxP technology. We targeted the expression of Cre-recombinase to the choroid plexus (the blood-cerebral spinal fluid barrier) using the lymphotropic papovavirus control region (LPVcr) and to brain endothelium (the blood-brain-barrier) using the proximal promoter region of the human von Willebrand Factor gene (hVWF-f). We verified that LPVcr restricts expression to the choroid plexus in adult mice by using the LPVcr to drive n-LacZ expression in transgenic mice. The LPV-Cre and hVWF-Cre plasmids were then constructed and tested for Cre-recombinase function in vitro, and subsequently used to create transgenic mice. The resulting transgenic mice were characterized for cell-type specific Cre-mediated endonuclease activity by crossing them with transgenic mice containing a loxP-flanked-LacZ/EGFP dual reporter gene Z/EG. The dual Cre-Z/EG transgenic offspring were evaluated for the location of EGFP mRNA expression by reverse transcriptase PCR and for protein expression by immunohistochemistry. Immunohistochemistry for EGFP verified expression in the target cells, and no ectopic expression outside of the expected cell types. The LPV-Cre.0607 transgenic line expressed functional Cre only in the choroid plexus and hVWF-Cre.1304 line in brain endothelium.
authors.affiliation: Department of Pharmaceutics, University of Washington, Box 357610, Seattle, WA 98195-7610, USA.
authors.forename: Matthew H
authors.initials: MH
authors.lastname: Crouthamel
authors.forename: Edward J
authors.initials: EJ
authors.lastname: Kelly
authors.forename: Rodney J Y
authors.initials: RJ
authors.lastname: Ho
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier; D002555:Cerebrospinal Fluid; D002831:Choroid Plexus / Q000502:physiology; D055786:Gene Knockout Techniques / Q000379:methods*; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D019426:Integrases / Q000235:genetics / Q000378:metabolism; D007763:Lac Operon; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009928:Organ Specificity; D011401:Promoter Regions, Genetic; D014841:von Willebrand Factor / Q000235:genetics
chemical_list: C485184:enhanced green fluorescent protein; D014841:von Willebrand Factor; D049452:Green Fluorescent Proteins; C045073:Cre recombinase; D019426:Integrases