title: A simplified polyethylenimine-mediated transfection process for large-scale and high-throughput applications.
abstract: Transient gene expression in mammalian cells is a valuable alternative to stable cell lines for the rapid production of large amounts of recombinant proteins. While the establishment of stable cell lines takes 2-6 months, milligram amounts of protein can be obtained within a week following transfection. The polycation polyethylenimine (PEI) is one of the most utilized reagents for small- to large-scale transfections as it is simple to use and, when combined with optimized expression vectors and cell lines, provides high transfection efficiency and titers. As with most transfection reagents, PEI-mediated transfection involves the formation of nanoparticles (polyplexes) which are obtained by its mixing with plasmid DNA. A short incubation period that allows polyplexes to reach their optimal size is performed prior to their addition to the culture. As the quality of polyplexes directly impacts transfection efficiency and productivity, their formation complicates scalability and automation of the process, especially when performed in large-scale bioreactors or small-scale high-throughput formats. To avoid variations in transfection efficiency and productivity that arise from polyplexes formation step, we have optimized the conditions for their creation directly in the culture by the consecutive addition of DNA and PEI. This simplified approach is directly transferable from suspension cultures grown in 6-well plates to shaker flasks and 5-L WAVE bioreactors. As it minimizes the number of steps and does not require an incubation period for polyplex formation, it is also suitable for automation using static cultures in 96-well plates. This "direct" transfection method thus provides a robust platform for both high-throughput expression and large-scale production of recombinant proteins.
authors.affiliation: Département de Biochimie, Faculté de Médecine, Université de Montréal, QC, Canada.
authors.forename: Céline
authors.initials: C
authors.lastname: Raymond
authors.forename: Roseanne
authors.initials: R
authors.lastname: Tom
authors.forename: Sylvie
authors.initials: S
authors.lastname: Perret
authors.forename: Pascal
authors.initials: P
authors.lastname: Moussouami
authors.forename: Denis
authors.initials: D
authors.lastname: L'Abbé
authors.forename: Gilles
authors.initials: G
authors.lastname: St-Laurent
authors.forename: Yves
authors.initials: Y
authors.lastname: Durocher
mesh_terms: D000818:Animals; D019149:Bioreactors; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D004247:DNA / Q000737:chemistry / Q000235:genetics; D015870:Gene Expression; D005822:Genetic Vectors / Q000737:chemistry; D057166:High-Throughput Screening Assays; D006801:Humans; D008322:Mammals; D053758:Nanoparticles / Q000737:chemistry; D010957:Plasmids / Q000737:chemistry / Q000235:genetics; D011094:Polyethyleneimine / Q000737:chemistry* / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D014162:Transfection / Q000379:methods*
chemical_list: D011994:Recombinant Proteins; D011094:Polyethyleneimine; D004247:DNA