title: In vitro susceptibility of Pythium insidiosum to macrolides and tetracycline antibiotics.
abstract: We describe the in vitro activity of macrolides and tetracycline antibiotics against Pythium insidiosum. The MICs were determined according to CLSI procedures (visual MIC) and by a colorimetric method [3-(4,5-dimethyl-2-thiazyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT)]. The lowest geometric mean (GM) MIC (MICs in μg/ml) (0.39 and 0.7 by visual reading and colorimetric method, respectively) and MIC ranges (0.125 to 2.0) were obtained for minocycline, while the highest MICs were shown for erythromycin (GM of 7.58 and 12.25 by visual reading and colorimetric method, respectively, and MIC ranged from 2 to 32). This significant in vitro activity makes these classes of antibiotics good candidates for experimental treatment of pythiosis.
authors.affiliation: Departamento de Microbiologia e Parasitologia, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul, Brazil.
authors.forename: Erico Silva
authors.initials: ES
authors.lastname: Loreto
authors.forename: Débora Alves Nunes
authors.initials: DA
authors.lastname: Mario
authors.forename: Laura Bedin
authors.initials: LB
authors.lastname: Denardi
authors.forename: Sydney Hartz
authors.initials: SH
authors.lastname: Alves
authors.forename: Janio Morais
authors.initials: JM
authors.lastname: Santurio
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004917:Erythromycin / Q000494:pharmacology; D018942:Macrolides / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D008911:Minocycline / Q000494:pharmacology; D011775:Pythium / Q000187:drug effects*; D013752:Tetracycline / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D018942:Macrolides; D004917:Erythromycin; D013752:Tetracycline; D008911:Minocycline