title: Cyathane diterpenes from Chinese mushroom Sarcodon scabrosus and their neurite outgrowth-promoting activity.
abstract: Two novel cyathane diterpenoids, designated scabronines K (1) and L (2), were isolated from the fruiting bodies of the basidiomycete Sarcodon scabrosus together with four known analogues, sarcodonins G (3), A (5), M (6), and scabronine H (4). Their structures were elucidated on the basis of extensive spectroscopic analysis including 2D-NMR (HMBC, HSQC, ROESY, (1)H,(1)H-COSY) and MS experiments. The isolated compounds were evaluated for nerve growth factor (NGF)-mediated neurite outgrowth using rat pheochromocytoma (PC12) cells as a model system of neuronal differentiation. Among these compounds, only sarcodonins G and A (3 and 5) at 25 μM showed significant neurite outgrowth (neuritegenesis)-promoting activity in the presence of 20 ng/mL NGF after 24h treatment. Their structure-neurite inducing activity relationship was also discussed.
authors.affiliation: Research Centre for Natural Medicinal Chemistry, College of Science, Northwest A&F University, Yangling, 712100 Shaanxi, China.
authors.forename: Xin-Wei
authors.initials: XW
authors.lastname: Shi
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.forename: Jin-Ming
authors.initials: JM
authors.lastname: Gao
authors.forename: An-Ling
authors.initials: AL
authors.lastname: Zhang
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D000818:Animals; D004224:Diterpenes / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D048690:Fruiting Bodies, Fungal / Q000737:chemistry; D009414:Nerve Growth Factors / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D016501:Neurites / Q000187:drug effects* / Q000502:physiology; D016716:PC12 Cells; D051381:Rats; D013329:Structure-Activity Relationship
chemical_list: D004224:Diterpenes; D009414:Nerve Growth Factors; C497675:cyathane; C410409:sarcodonin G