title: Inhibitory effect of ginsenosides from steamed ginseng-leaves and flowers on the LPS-stimulated IL-12 production in bone marrow-derived dendritic cells.
abstract: Interleukin-12, a heterodimeric cytokine comprising p40 and p35 subunits, plays an essential role in the regulating the differentiation of Th cells, which establish and maximize the capabilities of the immune system. The aim of present study is to screen the effect of 21 ginsenosides from steamed ginseng-leaves and flowers on IL-12 production in bone marrow-derived dendritic cells induced by lipopolysaccharide. Noticeably, ginsenoside Rg(6) (12) and ginsenoside F(4) (13) exhibited particularly inhibitory effect on LPS-induced IL-12 production with the inhibition values of 80 and 82%; and ginsenoside ST(1) (4), ginsenoside SL(2) (8), ginsenoside SL(3) (9), ginsenoside Rh(3) (14), ginsenoside Rk(2) (15), and ginsenoside Rs(4) (18) showed moderate effects with inhibition rates of 63, 65, 67, 68, 71, 73, and 67%, respectively. These results warrant further studies concerning potential of saponin extracts of steamed ginseng-leaves and flowers for medicinal uses.
authors.affiliation: College of Pharmacy, Chungnam National University, Daejeon, 305-764, Korea.
authors.forename: Nguyen Huu
authors.initials: NH
authors.lastname: Tung
authors.forename: Tran Hong
authors.initials: TH
authors.lastname: Quang
authors.forename: Jeong-Hyun
authors.initials: JH
authors.lastname: Son
authors.forename: Jung-Eun
authors.initials: JE
authors.lastname: Koo
authors.forename: Hye-Jin
authors.initials: HJ
authors.lastname: Hong
authors.forename: Young-Sang
authors.initials: YS
authors.lastname: Koh
authors.forename: Gyu Yong
authors.initials: GY
authors.lastname: Song
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Kim
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000276:immunology; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000187:drug effects* / Q000276:immunology; D004305:Dose-Response Relationship, Drug; D035264:Flowers / Q000737:chemistry; D036145:Ginsenosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018664:Interleukin-12 / Q000096:biosynthesis* / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D015394:Molecular Structure; D005894:Panax / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D028321:Plant Preparations / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D013227:Steam; D013329:Structure-Activity Relationship
chemical_list: D036145:Ginsenosides; D008070:Lipopolysaccharides; D028321:Plant Preparations; D013227:Steam; D018664:Interleukin-12