title: Evaluation of serum and urine 1,5-anhydro-D-glucitol and myo-inositol concentrations in healthy dogs.
abstract: 1,5-anhydro-D-glucitol (1,5AG) is a pyranoid polyol compound found in human circulating blood. Myo-inositol (MI) is a stereoisomer of inositol and serves as a precursor of inositol phospholipids. 1,5AG and MI are filtered by the glomerulus and almost completely reabsorbed through the renal tubules. However, under hyperglycemic conditions, reabsorption through the renal tubules is competitively inhibited because the structures of 1,5AG and MI resemble that of glucose. In this study, we investigated the kinetics of serum and urine 1,5AG and MI levels in healthy dogs. We demonstrated that 1,5AG and MI exist in canine serum and urine by gas chromatography-mass spectrometry. Under continuous hyperglycemic conditions, the serum 1,5AG concentration in healthy dogs decreased while the serum MI concentration remained unchanged. Urinary excretion of 1,5AG and MI increased significantly after blood glucose concentrations reached 200 to 220 mg/dl. A significant negative correlation was observed between serum 1,5AG and glucose concentrations during hyperglycemia. However, no significant correlation was observed between serum MI and glucose concentrations. In this study, we demonstrated that serum and urine 1,5AG and MI levels were changed by blood glucose concentrations. The serum 1,5AG concentration was decreased by continuous hyperglycemia. However, the serum MI concentration does not reflect hyperglycemia.
authors.affiliation: Department of Veterinary Nursing and Technology, Nippon Veterinary and Life Science University, 1–7–1 Kyonancho, Musashino, Tokyo 180–8602, Japan.
authors.forename: Yohei
authors.initials: Y
authors.lastname: Miki
authors.forename: Akihiro
authors.initials: A
authors.lastname: Mori
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Hayakawa
authors.forename: Tomoe
authors.initials: T
authors.lastname: Niki
authors.forename: Hitomi
authors.initials: H
authors.lastname: Oda
authors.forename: Kaori
authors.initials: K
authors.lastname: Saeki
authors.forename: Toko
authors.initials: T
authors.lastname: Sato
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tazaki
authors.forename: Katsumi
authors.initials: K
authors.lastname: Ishioka
authors.forename: Toshiro
authors.initials: T
authors.lastname: Arai
authors.forename: Toshinori
authors.initials: T
authors.lastname: Sako
mesh_terms: D000818:Animals; D001786:Blood Glucose; D003847:Deoxyglucose / Q000097:blood* / Q000652:urine*; D003920:Diabetes Mellitus / Q000097:blood / Q000652:urine / Q000662:veterinary; D004283:Dog Diseases / Q000097:blood / Q000188:drug therapy / Q000652:urine; D004285:Dogs; D005260:Female; D007294:Inositol / Q000097:blood* / Q000652:urine*; D007328:Insulin / Q000627:therapeutic use; D008297:Male; D019397:Pancreas, Artificial / Q000662:veterinary
chemical_list: D001786:Blood Glucose; D007328:Insulin; D007294:Inositol; C006584:1,5-anhydroglucitol; D003847:Deoxyglucose