title: Efficacy of ginkgolic acids against Cryptosporidium andersoni in cell culture.
abstract: Cryptosporidium is a worldwide waterborne parasite and the treatment is a severe problem in immunocompromised patients. In this study, we used the in vitro culture system to evaluate the anti-Cryptosporidium activity of ginkgolic acids (GAs), nitazoxanide (NTZ), garlicin (GAR), and artemether (ART). The growth of Cryptosporidium andersoni in HCT-8 cells was determined by real-time PCR assay. When exposed to 5.00 μg/ml GAs or 10.00 μg/ml NTZ for 48 h, the number of C. andersoni in cultures was on a very low lever, but the number of parasites did not significantly decrease when exposed to GAR and ART. Our results indicate that GAs could be a potential drug for the treatment of cryptosporidiosis.
authors.affiliation: School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang, 212013, People's Republic of China.
authors.forename: Liang
authors.initials: L
authors.lastname: Wu
authors.forename: Xu-gan
authors.initials: XG
authors.lastname: Jiang
authors.forename: Yu-juan
authors.initials: YJ
authors.lastname: Shen
authors.forename: Zhao-xi
authors.initials: ZX
authors.lastname: Lu
authors.forename: Guo-hua
authors.initials: GH
authors.lastname: Tu
authors.forename: Xing-li
authors.initials: XL
authors.lastname: Fu
authors.forename: Sheng-xia
authors.initials: SX
authors.lastname: Chen
authors.forename: Jian-ping
authors.initials: JP
authors.lastname: Cao
mesh_terms: D000498:Allyl Compounds / Q000494:pharmacology; D000981:Antiprotozoal Agents / Q000494:pharmacology*; D000077549:Artemether; D037621:Artemisinins / Q000494:pharmacology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003458:Cryptosporidium / Q000187:drug effects*; D004220:Disulfides / Q000494:pharmacology; D006801:Humans; D009574:Nitro Compounds; D021261:Parasitic Sensitivity Tests; D012459:Salicylates / Q000494:pharmacology*; D013844:Thiazoles / Q000494:pharmacology
chemical_list: D000498:Allyl Compounds; D000981:Antiprotozoal Agents; D037621:Artemisinins; D004220:Disulfides; D009574:Nitro Compounds; D012459:Salicylates; D013844:Thiazoles; C112485:ginkgolic acid; C028009:diallyl disulfide; D000077549:Artemether; C041747:nitazoxanide