title: Determination of malachite green residues in fish using a highly sensitive electrochemiluminescence method combined with molecularly imprinted solid phase extraction.
abstract: An electrochemiluminescence (ECL) inhibition method combined with molecularly imprinted solid phase extraction (MISPE) was developed for quantitative determination of malachite green (MG) residues in fish. It was found that MG could strongly inhibit the ECL signal of luminol. Under the optimized conditions, the quenched ECL intensity versus the logarithm of the concentration of MG was in good linear relationship over a concentration range from 20 to 5000 ppt. The method detection limit was found to be about 6 ppt. Molecularly imprinted polymers (MIPs) were synthesized as solid phase extraction (SPE) sorbents, and MISPE was used for the selective extraction and purification of MG. By carrying out the oxidation reaction with 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ), which could convert leucomalachite green (LMG) into MG, this method was successfully applied to determine MG residues in fish. A possible mechanism for the quenching effects of MG on luminol was also proposed.
authors.affiliation: Faculty of Materials Science and Chemical Engineering, The State Key Laboratory Base of Novel Functional Materials and Preparation Science, Ningbo University, Ningbo, Zhejiang, P R China. nbuguo@163.com
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Guo
authors.forename: Panpan
authors.initials: P
authors.lastname: Gai
authors.forename: Tingting
authors.initials: T
authors.lastname: Hao
authors.forename: Jing
authors.initials: J
authors.lastname: Duan
authors.forename: Sui
authors.initials: S
authors.lastname: Wang
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000032:analysis*; D004566:Electrodes; D005399:Fishes; D005506:Food Contamination / Q000032:analysis*; D049408:Luminescent Agents; D008163:Luminescent Measurements / Q000379:methods*; D008165:Luminol; D012394:Rosaniline Dyes / Q000032:analysis*
chemical_list: D000890:Anti-Infective Agents; D049408:Luminescent Agents; D012394:Rosaniline Dyes; C005095:malachite green; D008165:Luminol