title: Isolation and in vitro characterization of putative porcine embryonic stem cells from cloned embryos treated with trichostatin A.
abstract: We report here the establishment and characterization of putative porcine embryonic stem cell (ESC) lines derived from somatic cell nuclear transfer embryos (NT-ESCs). These cells had a similar morphology to that described previously by us for ESCs derived from in vitro produced embryos, namely, a polygonal shape, a relatively small (10-15 μm) diameter, a small cytoplasmic/nuclear ratio, a single nucleus with multiple nucleoli and multiple lipid inclusions in the cytoplasm. NT-ESCs could be passaged at least 15 times and vitrified repeatedly without changes in their morphology, karyotype, or Oct-4 and Nanog expression. These cells formed embryoid bodies and could be directed to differentiate in vitro to cell types representative of all three germ layers. Following their injection into blastocysts, these cells preferentially localized in the inner cell mass. In conclusion, we have isolated putative porcine ESCs from cloned embryos that have the potential to be used for a variety of applications including as a model for human therapeutic cloning.
authors.affiliation: Reproductive Biotechnology Group, Robinson Institute, Centre for Stem Cell Research and School of Paediatrics and Reproductive Health, University of Adelaide, Australia.
authors.forename: Ivan
authors.initials: I
authors.lastname: Vassiliev
authors.forename: Svetlana
authors.initials: S
authors.lastname: Vassilieva
authors.forename: Kam P
authors.initials: KP
authors.lastname: Truong
authors.forename: Luke F S
authors.initials: LF
authors.lastname: Beebe
authors.forename: Stephen M
authors.initials: SM
authors.lastname: McIlfatrick
authors.forename: Sharon J
authors.initials: SJ
authors.lastname: Harrison
authors.forename: Mark B
authors.initials: MB
authors.lastname: Nottle
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000187:drug effects; D002460:Cell Line; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D019976:Cloning, Organism / Q000379:methods; D004622:Embryo, Mammalian / Q000166:cytology* / Q000187:drug effects*; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000187:drug effects*; D005260:Female; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology; D006877:Hydroxamic Acids / Q000494:pharmacology*; D007621:Karyotyping; D023421:Models, Animal; D013552:Swine
chemical_list: D056572:Histone Deacetylase Inhibitors; D006877:Hydroxamic Acids; C012589:trichostatin A