title: [Progress on DNA quantification in estimation of postmortem interval].
abstract: Estimation of postmortem interval (PMI) is one of the difficult problems in forensic medicine. With the development of molecular biological techniques, DNA quantification methods were widely applied in estimating PMI. The postmortem degradation of DNA in different tissues and organs was discussed in this article and the recent DNA quantitative techniques being used for estimating PMI were reviewed. These techniques included single cell gel electrophoresis, Feulgen staining image analysis, flow cytometry.
authors.affiliation: Department of Forensic Medicine, School of Basic Medical Science, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Lin
authors.forename: Ya-sha
authors.initials: YS
authors.lastname: Yin
authors.forename: Qiang
authors.initials: Q
authors.lastname: Ji
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D020552:Comet Assay / Q000379:methods*; D004247:DNA / Q000032:analysis*; D053836:DNA Degradation, Necrotic; D005434:Flow Cytometry; D049429:Forensic Pathology; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007668:Kidney / Q000378:metabolism / Q000473:pathology; D011180:Postmortem Changes; D012394:Rosaniline Dyes; D013154:Spleen / Q000378:metabolism / Q000473:pathology; D013696:Temperature; D013997:Time Factors
chemical_list: C007722:Feulgen stain; D012394:Rosaniline Dyes; D004247:DNA