title: Two-photon mapping of neural circuits.
abstract: For the study of neural circuits, it would be ideal to be able to discern in a living preparation the interconnections among neurons. Working toward that aim, we have developed a method, using two-photon uncaging of glutamate in brain slices, by which we can detect neurons that are presynaptic to any particular cell. The method, described here, has single-cell resolution and can be applied systematically and quickly to test entire populations of neurons, making it possible to perform online functional analysis of connectivity.
authors.forename: Volodymyr
authors.initials: V
authors.lastname: Nikolenko
authors.forename: Elodie
authors.initials: E
authors.lastname: Fino
authors.forename: Rafael
authors.initials: R
authors.lastname: Yuste
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology*; D018698:Glutamic Acid / Q000378:metabolism; D006801:Humans; D008853:Microscopy / Q000379:methods*; D009474:Neurons / Q000737:chemistry / Q000166:cytology* / Q000502:physiology*; D018377:Neurotransmitter Agents / Q000378:metabolism; D017785:Photons
chemical_list: D018377:Neurotransmitter Agents; D018698:Glutamic Acid