title: [Molecular mechanism of protective effect of puerarin solid lipid nanoparticle on cerebral ischemia-reperfusion injury in gerbils].
abstract: OBJECTIVE
  To investigate the effects of puerarin solid lipid nanoparticle on fore brain ischemic-reperfusion injury in gerbils and it's mechanisms.
  
  
  METHODS
  Gerbils were randomly divided into 4 groups: sham group, cerebral ischemia-reperfusion injury group, puerarin solid lipid nanoparticle group and puerarin injection control group. The gerbils' cerebral ischemia-reperfusion injury model was constructed with ligating bilater carotids method. The histomorphology and Bcl-2, Caspase-3 and HSP70 expressions were detected by HE dyeing and immunohistochemical method.
  
  
  RESULTS
  After 24 h ischemia and reperfusion in gerbils, the level of Bcl-2 and HSP70 expressions in puerarin solid lipid nanoparticle group increased (P < 0.01) compared with the ischemic-reperfusion model group, and the level of Caspase-3 expression decreased (P < 0.01). The same results was consistent in puerarin injection control group.
  
  
  CONCLUSIONS
  Puerarin solid lipid nanoparticle group can protect the cerebral ischemia-reperfusion injury in gerbils, which may be related to the upregulation of Bcl-2 and HSP70 expression and downregulation of Caspase-3 expression.
authors.affiliation: Drug Research Center, Guangzhou Medical College, Guangzhou 510182, China. misszhuliu@163.com
authors.forename: Liu
authors.initials: L
authors.lastname: Zhu
authors.forename: Cheng-Feng
authors.initials: CF
authors.lastname: Luo
authors.forename: Mu
authors.initials: M
authors.lastname: Yuan
authors.forename: Min-Sheng
authors.initials: MS
authors.lastname: Chen
authors.forename: Hong
authors.initials: H
authors.lastname: Ji
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D002545:Brain Ischemia / Q000188:drug therapy / Q000378:metabolism / Q000517:prevention & control*; D053148:Caspase 3 / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D005849:Gerbillinae; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D007150:Immunohistochemistry; D007529:Isoflavones / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D008055:Lipids / Q000737:chemistry; D008297:Male; D053758:Nanoparticles / Q000737:chemistry; D010316:Particle Size; D020011:Protective Agents / Q000008:administration & dosage / Q000494:pharmacology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D029908:Pueraria / Q000737:chemistry; D011897:Random Allocation; D015427:Reperfusion Injury / Q000188:drug therapy / Q000378:metabolism / Q000517:prevention & control*
chemical_list: D018840:HSP70 Heat-Shock Proteins; D007529:Isoflavones; D008055:Lipids; D020011:Protective Agents; D019253:Proto-Oncogene Proteins c-bcl-2; D053148:Caspase 3; C033607:puerarin