title: Growth stimulatory effect of interleukin (IL)-1 alpha produced by oral squamous carcinoma cell lines.
abstract: The expression of IL-1 alpha and its effect on the cell growth were examined in six human oral squamous carcinoma cell lines. All the cell lines expressed IL-1 alpha mRNA and protein at various levels. Particularly, HSC-2 and HSC-3 cells showed high level of the mRNA expression and secreted large amounts of IL-1 alpha into the culture fluid. Scatchard plot analysis of IL-1 alpha binding revealed that HSC-2 cells had high-and low-affinity receptors, whereas IL-1 alpha receptors on HSC-3 cells were of undetectable level. The cell growth of HSC-2 and HSC-3 cells was stimulated by IL-1 alpha and inhibited by anti-IL-1 alpha antibody or IL-1 receptor antagonist. The expression of IL-1 alpha mRNA by these cell lines was induced by either IL-1 alpha, epidermal growth factor (EGF) or transforming growth factor alpha (TGF-alpha). On the other hand, IL-1 alpha promoted the mRNA expression of TGF-alpha and EGF receptor. These findings indicate that IL-1 alpha acts as an autocrine growth stimulator for oral squamous carcinoma cells in vitro and its interaction with EGF/TGF-alpha/receptor system may play a role in this enhanced growth by IL-1 alpha.
authors.affiliation: HIROSHIMA UNIV,SCH MED,DEPT PATHOL 1,MINAMI KU,HIROSHIMA 734,JAPAN. HIROSHIMA UNIV,SCH MED,DEPT PARASITOL,MINAMI KU,HIROSHIMA 734,JAPAN. HIROSHIMA UNIV,SCH DENT,DEPT ORAL PATHOL,MINAMI KU,HIROSHIMA 734,JAPAN.
authors.forename: R
authors.initials: R
authors.lastname: Ito
authors.forename: W
authors.initials: W
authors.lastname: Yasui
authors.forename: H
authors.initials: H
authors.lastname: Yokozaki
authors.forename: U
authors.initials: U
authors.lastname: Yamashita
authors.forename: T
authors.initials: T
authors.lastname: Takata
authors.forename: H
authors.initials: H
authors.lastname: Nikai
authors.forename: E
authors.initials: E
authors.lastname: Tahara