title: Activity of a dry mist-generated hydrogen peroxide disinfection system against methicillin-resistant Staphylococcus aureus and Acinetobacter baumannii.
abstract: BACKGROUND
  The aim of this study was to evaluate the activity of a dry mist-generated hydrogen peroxide (DMHP) system (Sterinis; Gloster Sante Europe, Labege cedex, France) against methicillin-resistant Staphylococcus aureus (MRSA) and Acinetobacter baumannii.
  
  
  METHODS
  McFarland 0.5 suspensions of 2 test bacteria, either pure or containing 5% sterile serum, were prepared and inoculated onto sterile stainless steel disks. Each disk in a Petri dish-with the Petri dish cover either closed or open-was placed in different locations in an intensive care unit room. Quantitative cultures were performed after the cycle.
  
  
  RESULTS
  No growth occurred on the disks in the absence of a barrier, except 1 disk containing serum. Existence of a barrier, as a drawer or a covered Petri dish, caused failure in the disinfection activity. The mean reduction in initial log(10) bacterial count was lower for both of the test bacteria in presence of a barrier: 4.44- to 4.70-log(10) colony-forming units (cfu) decrease was observed in absence of a barrier, whereas 1.49- to 3.79-log(10) cfu decrease was observed in presence of a barrier. When the culture results were compared according to organic load content, the mean (±standard deviation) reduction of initial contamination in pure and in serum containing MRSA suspensions was 4.25 ± 1.20- and 3.34 ± 1.89-log(10) cfu, respectively. The mean (±standard deviation) reduction in pure and in serum containing A baumannii suspensions was 4.34 ± 0.89- and 3.87 ± 1.26-log(10) cfu, respectively. The differences were statistically significant (P < .001).
  
  
  CONCLUSIONS
  Sterinis was capable of killing MRSA and A baumannii on open surfaces; however, it was not effective in closed or semiclosed areas. Presence of serum also caused failure in the disinfection activity of the system.
authors.affiliation: Department of Infectious Diseases and Clinical Microbiology, Zonguldak Karaelmas University, Turkey. nihal_piskin@yahoo.com
authors.forename: Nihal
authors.initials: N
authors.lastname: Piskin
authors.forename: Guven
authors.initials: G
authors.lastname: Celebi
authors.forename: Canan
authors.initials: C
authors.lastname: Kulah
authors.forename: Zafer
authors.initials: Z
authors.lastname: Mengeloglu
authors.forename: Mehtap
authors.initials: M
authors.lastname: Yumusak
mesh_terms: D040981:Acinetobacter baumannii / Q000187:drug effects*; D000336:Aerosols / Q000494:pharmacology*; D015169:Colony Count, Microbial; D004202:Disinfectants / Q000494:pharmacology*; D004203:Disinfection / Q000379:methods*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects*; D050296:Microbial Viability / Q000187:drug effects; D044967:Serum / Q000378:metabolism
chemical_list: D000336:Aerosols; D004202:Disinfectants; D006861:Hydrogen Peroxide