title: Herpesviral replication compartments move and coalesce at nuclear speckles to enhance export of viral late mRNA.
abstract: The role of the intranuclear movement of chromatin in gene expression is not well-understood. Herpes simplex virus forms replication compartments (RCs) in infected cell nuclei as sites of viral DNA replication and late gene transcription. These structures develop from small compartments that grow in size, move, and coalesce. Quantitative analysis of RC trajectories, derived from 4D images, shows that most RCs move by directed motion. Directed movement is impaired in the presence of actin and myosin inhibitors as well as a transcription inhibitor. In addition, RCs coalesce at and reorganize nuclear speckles. Lastly, distinct effects of actin and myosin inhibitors on viral gene expression suggest that RC movement is not required for transcription, but rather, movement results in the bridging of transcriptionally active RCs with nuclear speckles to form structures that enhance export of viral late mRNAs.
authors.affiliation: Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Lynne
authors.initials: L
authors.lastname: Chang
authors.forename: William J
authors.initials: WJ
authors.lastname: Godinez
authors.forename: Il-Han
authors.initials: IH
authors.lastname: Kim
authors.forename: Marco
authors.initials: M
authors.lastname: Tektonidis
authors.forename: Primal
authors.initials: P
authors.lastname: de Lanerolle
authors.forename: Roland
authors.initials: R
authors.lastname: Eils
authors.forename: Karl
authors.initials: K
authors.lastname: Rohr
authors.forename: David M
authors.initials: DM
authors.lastname: Knipe
mesh_terms: D021581:Active Transport, Cell Nucleus; D000818:Animals; D002467:Cell Nucleus; D002522:Chlorocebus aethiops; D002843:Chromatin; D006564:Herpesviridae / Q000502:physiology*; D012367:RNA, Viral / Q000378:metabolism*; D014158:Transcription, Genetic; D014162:Transfection; D014709:Vero Cells; D014779:Virus Replication
chemical_list: D002843:Chromatin; D012367:RNA, Viral