title: New lipidomic approaches in cystic fibrosis.
abstract: Lipid analysis has been a crucial source of information in cystic fibrosis (CF). New methodologies for qualitative and quantitative lipidomics allow evaluation of a large number of samples, of special interest in patient screening for diagnostic and prognostic biological markers, as well as in cell physiology. In this chapter, two new complementary approaches are described: matrix-assisted laser desorption coupled to time of flight (MALDI-TOF-ClinProTools™) and liquid chromatography coupled to ion trap mass spectrometry (LC-MS( n )). MALDI-TOF-ClinProTools™ offers a large unbiased screening for the discovery of potential lipid alterations in diseased patients. LC-MS( n ) represents a state-of-the-art lipidomic tool for the identification and quantification of such alterations. The combination of both may open new perspectives in the quest for lipids participating in CF pathogenesis, therapy targets, and biomarkers.
authors.affiliation: INSERM U845, Université Paris Descartes, Paris, France. mario.ollero@inserm.fr
authors.forename: Mario
authors.initials: M
authors.lastname: Ollero
authors.forename: Ida Chiara
authors.initials: IC
authors.lastname: Guerrera
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Astarita
authors.forename: Daniele
authors.initials: D
authors.lastname: Piomelli
authors.forename: Aleksander
authors.initials: A
authors.lastname: Edelman
mesh_terms: D015415:Biomarkers / Q000032:analysis*; D002461:Cell Line, Transformed; D002853:Chromatography, Liquid; D003550:Cystic Fibrosis / Q000097:blood* / Q000175:diagnosis / Q000503:physiopathology; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006801:Humans; D062385:Hydroxybenzoates / Q000737:chemistry; D050356:Lipid Metabolism; D008055:Lipids / Q000032:analysis / Q000096:biosynthesis / Q000737:chemistry; D009154:Mutation; D040901:Proteomics / Q000379:methods; D012015:Reference Standards; D012997:Solvents / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014162:Transfection
chemical_list: D015415:Biomarkers; D062385:Hydroxybenzoates; D008055:Lipids; D012997:Solvents; D019005:Cystic Fibrosis Transmembrane Conductance Regulator