title: Direct detection of equine herpesvirus type 1 DNA in nasal swabs by loop-mediated isothermal amplification (LAMP).
abstract: We evaluated loop-mediated isothermal amplification (LAMP) as a means of detecting equine herpesvirus type 1 (EHV-1) DNA directly from nasal swabs. To increase the sensitivity, we added a step in which the samples were heat-treated to the original LAMP procedure. The detection limit of the LAMP assay with heat treatment was 10 times more sensitive than the original LAMP assay even when the DNA extraction step was omitted. In addition, the LAMP assay with heat treatment was more sensitive than the original LAMP assay and the polymerase chain reaction using clinical samples. The LAMP assay with heat treatment is easy to perform and so should be applicable to the diagnosis of EHV-1 infections in clinical laboratories.
authors.affiliation: Epizootic Research Center, Equine Research Institute, Japan Racing Association, 1400–4 Shiba, Shimotsuke, Tochigi 329–0412, Japan. nemoto_manabu@epizoo.equinst.go.jp
authors.forename: Manabu
authors.initials: M
authors.lastname: Nemoto
authors.forename: Minoru
authors.initials: M
authors.lastname: Ohta
authors.forename: Koji
authors.initials: K
authors.lastname: Tsujimura
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Bannai
authors.forename: Takashi
authors.initials: T
authors.lastname: Yamanaka
authors.forename: Takashi
authors.initials: T
authors.lastname: Kondo
authors.forename: Tomio
authors.initials: T
authors.lastname: Matsumura
mesh_terms: D000818:Animals; D004279:DNA, Viral / Q000302:isolation & purification*; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D004861:Herpesvirus 1, Equid / Q000235:genetics* / Q000302:isolation & purification; D006734:Horse Diseases / Q000175:diagnosis / Q000821:virology*; D006736:Horses; D009093:Mucus / Q000821:virology*; D021141:Nucleic Acid Amplification Techniques / Q000662:veterinary*; D012680:Sensitivity and Specificity
chemical_list: D004279:DNA, Viral