title: Characteristic resistance mechanism of Mycobacterium tuberculosis to DC-159a, a new respiratory quinolone.
abstract: A G88C mutation in GyrA is one of the key alterations by which Mycobacterium tuberculosis mutants acquire DC-159a resistance in vitro. A novel double mutation in GyrA, G88C D94H, conferred high DC-159a resistance. Different mutation patterns in GyrA were demonstrated for DC-159a-resistant mutants and quinolone-resistant multidrug-resistant (QR-MDR) M. tuberculosis isolates, with a mutation either at position 90 or 94 and double mutations at 90 and 91 or at 90 and 94. DC-159a might be promising for QR M. tuberculosis treatment.
authors.affiliation: Department of Mycobacteria References and Research, Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association, 3-1-24 Matsuyama, Kiyose, Tokyo 204-8533, Japan.
authors.forename: Jun-ichiro
authors.initials: J
authors.lastname: Sekiguchi
authors.forename: Areeya
authors.initials: A
authors.lastname: Disratthakit
authors.forename: Shinji
authors.initials: S
authors.lastname: Maeda
authors.forename: Norio
authors.initials: N
authors.lastname: Doi
mesh_terms: D000631:Aminopyridines / Q000494:pharmacology*; D000995:Antitubercular Agents / Q000494:pharmacology*; D027081:DNA Gyrase / Q000235:genetics*; D004278:DNA, Superhelical / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics; D024841:Fluoroquinolones / Q000494:pharmacology*; D006801:Humans; D008826:Microbial Sensitivity Tests; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D018088:Tuberculosis, Multidrug-Resistant / Q000382:microbiology
chemical_list: C530475:7-(7-amino-7-methyl-5-azaspiro(2.4)heptan-5-yl)-6-fluoro-1-(2-fluoro-1-cyclopropyl)-1,4-dihydro-8-methoxy-4-oxo-3-quinolinecarboxylic acid; D000631:Aminopyridines; D000995:Antitubercular Agents; D004278:DNA, Superhelical; D024841:Fluoroquinolones; D027081:DNA Gyrase