title: Immunodepletion of albumin and immunoglobulin G from bovine plasma.
abstract: Current MS-based proteomics has facilitated the identification of large numbers of proteins from complex mixtures. The bovine plasma proteome has the potential to provide a wealth of information concerning the biological state of an animal. However, during MS-based experiments, higher abundance proteins such as albumin and immunoglobulin G (IgG) can hinder the identification of potentially important proteins that are present in much lower abundance. While a variety of readily available technologies exist for the depletion of multiple high-abundance proteins from human, mouse and rat samples, there are few available for bovine. In this study, we report the depletion of >97% of albumin and >92% of IgG from bovine plasma.
authors.affiliation: Animal and Bioscience Research Department, Animal and Grassland Research and Innovation Centre, Teagasc, Athenry, Co., Galway, Ireland.
authors.forename: Simon
authors.initials: S
authors.lastname: Faulkner
authors.forename: Giuliano
authors.initials: G
authors.lastname: Elia
authors.forename: Mark
authors.initials: M
authors.lastname: Hillard
authors.forename: Padraic
authors.initials: P
authors.lastname: O'Boyle
authors.forename: Michael
authors.initials: M
authors.lastname: Dunn
authors.forename: Dermot
authors.initials: D
authors.lastname: Morris
mesh_terms: D000818:Animals; D055910:Antibodies, Immobilized / Q000378:metabolism; D002417:Cattle / Q000097:blood*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D007074:Immunoglobulin G / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D007163:Immunosorbent Techniques; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D012710:Serum Albumin, Bovine / Q000032:analysis / Q000302:isolation & purification*
chemical_list: D055910:Antibodies, Immobilized; D007074:Immunoglobulin G; D012710:Serum Albumin, Bovine