title: [Variability in low-molecular subproteome of blood serum of healthy man in normal life conditions].
abstract: For analysis of inter-individual variability in low-molecular serum subproteome proteome profiles of healthy men at the age of 20-30 years (36 subjects), 30-40 years (11 subjects) and 40-50 years (11 subjects) were obtained. Serum samples were fractionated on magnetic beads MB WCX using ClinProt robot prior to mass-spectrometry based profiling. Mass-spectra were obtained with time-of-flight mass-spectrometer Autoflex III ("Bruker Daltonics") in automatic mode. It was shown that low-molecular serum subproteome of healthy humans was characterized by significant inter-individual variability. 21% of all peaks in proteome profiles had coefficient of variation more than 50% and 29% of all peaks had low dispersion (CV < 30%).Therefore majority of peaks in proteome profile were peaks with moderate inter-individual variability (CV from 30% to 50%). Fragments of high-molecular kininogen, inter-alpha-trypsin inhibitor, complement components C3 and C4a, apolipoprotein CI, platelet factor IV, beta2-microglobulin and cystatin C showed wide variation among examined groups of healthy men. Dispersion of high-molecular kininogen, inter-alpha-trypsin inhibitor, apolipoproteins AII and CIII peaks increased with age.
authors.forename: N A
authors.initials: NA
authors.lastname: Pakharukova
authors.forename: L Kh
authors.initials: LKh
authors.lastname: Pastushkova
authors.forename: O P
authors.initials: OP
authors.lastname: Trifonova
authors.forename: S A
authors.initials: SA
authors.lastname: Moshkovskiĭ
authors.forename: I M
authors.initials: IM
authors.lastname: Larina
mesh_terms: D000328:Adult; D000375:Aging / Q000097:blood*; D000704:Analysis of Variance; D015415:Biomarkers / Q000097:blood*; D001798:Blood Proteins / Q000032:analysis*; D003627:Data Interpretation, Statistical; D006801:Humans; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D020543:Proteome / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry; D055815:Young Adult
chemical_list: D015415:Biomarkers; D001798:Blood Proteins; D020543:Proteome