title: The complete genome sequence of 'Candidatus Liberibacter solanacearum', the bacterium associated with potato zebra chip disease.
abstract: Zebra Chip (ZC) is an emerging plant disease that causes aboveground decline of potato shoots and generally results in unusable tubers. This disease has led to multi-million dollar losses for growers in the central and western United States over the past decade and impacts the livelihood of potato farmers in Mexico and New Zealand. ZC is associated with 'Candidatus Liberibacter solanacearum', a fastidious alpha-proteobacterium that is transmitted by a phloem-feeding psyllid vector, Bactericera cockerelli Sulc. Research on this disease has been hampered by a lack of robust culture methods and paucity of genome sequence information for 'Ca. L. solanacearum'. Here we present the sequence of the 1.26 Mbp metagenome of 'Ca. L. solanacearum', based on DNA isolated from potato psyllids. The coding inventory of the 'Ca. L. solanacearum' genome was analyzed and compared to related Rhizobiaceae to better understand 'Ca. L. solanacearum' physiology and identify potential targets to develop improved treatment strategies. This analysis revealed a number of unique transporters and pathways, all potentially contributing to ZC pathogenesis. Some of these factors may have been acquired through horizontal gene transfer. Taxonomically, 'Ca. L. solanacearum' is related to 'Ca. L. asiaticus', a suspected causative agent of citrus huanglongbing, yet many genome rearrangements and several gene gains/losses are evident when comparing these two Liberibacter. species. Relative to 'Ca. L. asiaticus', 'Ca. L. solanacearum' probably has reduced capacity for nucleic acid modification, increased amino acid and vitamin biosynthesis functionalities, and gained a high-affinity iron transport system characteristic of several pathogenic microbes.
authors.affiliation: United States Department of Agriculture-Agricultural Research Service, CDPG, San Joaquin Valley Agricultural Sciences Center, Parlier, California, United States of America. hong.lin@ars.usda.gov
authors.forename: Hong
authors.initials: H
authors.lastname: Lin
authors.forename: Binghai
authors.initials: B
authors.lastname: Lou
authors.forename: Jonathan M
authors.initials: JM
authors.lastname: Glynn
authors.forename: Harshavardhan
authors.initials: H
authors.lastname: Doddapaneni
authors.forename: Edwin L
authors.initials: EL
authors.lastname: Civerolo
authors.forename: Chuanwu
authors.initials: C
authors.lastname: Chen
authors.forename: Yongping
authors.initials: Y
authors.lastname: Duan
authors.forename: Lijuan
authors.initials: L
authors.lastname: Zhou
authors.forename: Cheryl M
authors.initials: CM
authors.lastname: Vahling
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D001692:Biological Transport / Q000235:genetics; D050260:Carbohydrate Metabolism / Q000235:genetics; D002455:Cell Division / Q000235:genetics; D049109:Cell Proliferation; D002957:Citrus / Q000382:microbiology; D004261:DNA Replication / Q000235:genetics; D004269:DNA, Bacterial / Q000096:biosynthesis / Q000378:metabolism; D004734:Energy Metabolism / Q000235:genetics; D016680:Genome, Bacterial / Q000235:genetics*; D023281:Genomics; D009584:Nitrogen / Q000378:metabolism; D009711:Nucleotides / Q000378:metabolism; D010935:Plant Diseases / Q000382:microbiology*; D039002:Prophages / Q000235:genetics; D020560:Proteobacteria / Q000166:cytology / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D011198:Solanum tuberosum / Q000382:microbiology*; D013455:Sulfur / Q000378:metabolism; D014815:Vitamins / Q000096:biosynthesis / Q000378:metabolism
chemical_list: D000596:Amino Acids; D004269:DNA, Bacterial; D009711:Nucleotides; D014815:Vitamins; D013455:Sulfur; D009584:Nitrogen