title: Human dental pulp cell responses to new calcium silicate-based endodontic materials.
abstract: OBJECTIVE
  To evaluate human dental pulp cell responses to radiopaque dicalcium silicate cement and white-coloured mineral trioxide aggregate (WMTA).
  
  
  METHODS
  Flow cytometry was employed to quantify the phase percentage of pulp cell cycle. Alamar Blue was used for real-time and repeated monitoring of cell proliferation. Reverse transcription-polymerase chain reaction was performed to determine gene expression in pulp cells cultured on the cements.
  
  
  RESULTS
  The cells cultured on the radiopaque dicalcium silicate cement had similar S and G2 phases in the cell cycle and proliferation to WMTA at all culture times. In addition, the two materials presented the same evolution with similar values in interleukin-1, inducible nitric oxide synthase, alkaline phosphatase, osteocalcin and bone sialoprotein gene expression at all culture times.
  
  
  CONCLUSIONS
  The dental pulp cell responses to radiopaque dicalcium silicate cement were similar to those reported for WMTA in terms of cell cycle, proliferation, immunocompatibility and osteogenic differentiation.
authors.affiliation: Department of Dentistry, Chung Shan Medical University Hospital, Taichung City, Taiwan.
authors.forename: C C
authors.initials: CC
authors.lastname: Chen
authors.forename: M Y
authors.initials: MY
authors.lastname: Shie
authors.forename: S J
authors.initials: SJ
authors.lastname: Ding
mesh_terms: D000469:Alkaline Phosphatase / Q000187:drug effects / Q000378:metabolism; D017607:Aluminum Compounds / Q000494:pharmacology*; D000704:Analysis of Variance; D017610:Calcium Compounds / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D003738:Dental Cements / Q000494:pharmacology; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D004338:Drug Combinations; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D058574:Integrin-Binding Sialoprotein / Q000187:drug effects / Q000378:metabolism; D007375:Interleukin-1 / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000187:drug effects / Q000378:metabolism; D010006:Osteoblasts / Q000187:drug effects; D015675:Osteocalcin / Q000187:drug effects / Q000378:metabolism; D010087:Oxides / Q000494:pharmacology*; D061251:Primary Cell Culture; D012387:Root Canal Filling Materials / Q000494:pharmacology*; D017640:Silicates / Q000494:pharmacology*
chemical_list: D017607:Aluminum Compounds; D017610:Calcium Compounds; D003738:Dental Cements; D004338:Drug Combinations; D058574:Integrin-Binding Sialoprotein; D007375:Interleukin-1; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; C086631:mineral trioxide aggregate; C013481:dicalcium silicate; D015675:Osteocalcin; D052247:Nitric Oxide Synthase Type II; D000469:Alkaline Phosphatase