title: Serotonin-enhanced hyperactivation of hamster sperm.
abstract: The effects of serotonin on reproductive function were examined using hamster spermatozoa. When serotonin at concentrations from 1 fmol/l to 1 μmol/l was added to modified Tyrode's albumin lactate pyruvate (mTALP) medium, hyperactivation was significantly enhanced. Agonists and antagonists of 5-hydroxytryptamine hydrochloride (5-HT) receptors (5-HT(2) and 5-HT(4) receptors) were added to the medium. Both 5-HT(2) and 5-HT(4) receptor agonists significantly enhanced hyperactivation, although the effect was greater than the former. However, both 5-HT(2) and 5-HT(4) receptor antagonists significantly suppressed serotonin-enhanced hyperactivation, with the former suppressing stimulation by a lower concentration of serotonin than the latter. These results indicate that serotonin enhances hyperactivation via 5-HT(2) and/or 5-HT(4) receptors in a dose-dependent manner.
authors.affiliation: Department of Physiology, School of Medicine, Dokkyo Medical University, Mibu, Tochigi 321-0293, Japan. fujinoki@dokkyomed.ac.jp
authors.forename: Masakatsu
authors.initials: M
authors.lastname: Fujinoki
mesh_terms: D000818:Animals; D020013:Calcium Signaling; D006224:Cricetinae; D016895:Culture Media, Serum-Free; D004822:Epididymis / Q000166:cytology; D008297:Male; D008647:Mesocricetus; D009994:Osmolar Concentration; D012701:Serotonin / Q000378:metabolism*; D058826:Serotonin 5-HT2 Receptor Agonists / Q000494:pharmacology; D058830:Serotonin 5-HT2 Receptor Antagonists / Q000494:pharmacology; D058828:Serotonin 5-HT4 Receptor Agonists / Q000494:pharmacology; D058832:Serotonin 5-HT4 Receptor Antagonists / Q000494:pharmacology; D013075:Sperm Capacitation / Q000187:drug effects; D013081:Sperm Motility / Q000187:drug effects; D013082:Sperm Tail / Q000187:drug effects / Q000502:physiology; D013094:Spermatozoa / Q000187:drug effects / Q000502:physiology*; D013997:Time Factors
chemical_list: D016895:Culture Media, Serum-Free; D058826:Serotonin 5-HT2 Receptor Agonists; D058830:Serotonin 5-HT2 Receptor Antagonists; D058828:Serotonin 5-HT4 Receptor Agonists; D058832:Serotonin 5-HT4 Receptor Antagonists; D012701:Serotonin