title: Probing β-(1→3)-D-glucans interactions with recombinant human receptors using high-resolution NMR studies.
abstract: A full characterization of the high-resolution NMR spectrum of the laminarihexaose is described and used for the determination of the binding epitope of the more complex but structurally related laminarin. These biophysical data extend the current knowledge of β-glucans/Dectin-1 interactions and suggest different biological mechanisms in close relation with the size of the saccharidic chain.
authors.affiliation: Ecole Nationale Supérieure de Chimie de Rennes, CNRS, UMR 6226, Avenue du Général Leclerc, CS 50837, 35708 Rennes Cedex 7, France.
authors.forename: Balla
authors.initials: B
authors.lastname: Sylla
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Guégan
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Wieruszeski
authors.forename: Caroline
authors.initials: C
authors.lastname: Nugier-Chauvin
authors.forename: Laurent
authors.initials: L
authors.lastname: Legentil
authors.forename: Richard
authors.initials: R
authors.lastname: Daniellou
authors.forename: Vincent
authors.initials: V
authors.lastname: Ferrières
mesh_terms: D001665:Binding Sites; D002240:Carbohydrate Sequence; D018604:Epitope Mapping; D005936:Glucans; D006801:Humans; D007155:Immunologic Factors / Q000737:chemistry* / Q000378:metabolism; D037181:Lectins, C-Type; D016177:Macrophage-1 Antigen / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008565:Membrane Proteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D009844:Oligosaccharides / Q000737:chemistry* / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D011994:Recombinant Proteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D013329:Structure-Activity Relationship; D047071:beta-Glucans / Q000737:chemistry* / Q000378:metabolism; D000099181:Dectin-1
chemical_list: D005936:Glucans; D007155:Immunologic Factors; D037181:Lectins, C-Type; D016177:Macrophage-1 Antigen; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D009844:Oligosaccharides; D011134:Polysaccharides; D011994:Recombinant Proteins; D047071:beta-Glucans; D000099181:Dectin-1; C086571:laminarihexaose; C008247:laminaran