title: Engineering of a redox protein for DNA-directed assembly.
abstract: Engineered enzyme conjugate of the small laccase enzyme from Streptomyces coelicolor and zinc finger DNA binding domain from Zif268 is demonstrated to bind double stranded DNA in a site specific manner while retaining enzymatic activity.
authors.affiliation: Department of Chemical Engineering, Columbia University, 500 W 120th Street, New York, NY 10027, USA. geza.szilvay@vtt.fi
authors.forename: Géza R
authors.initials: GR
authors.lastname: Szilvay
authors.forename: Shayna
authors.initials: S
authors.lastname: Brocato
authors.forename: Dmitri
authors.initials: D
authors.lastname: Ivnitski
authors.forename: Carol
authors.initials: C
authors.lastname: Li
authors.forename: Pablo
authors.initials: P
authors.lastname: De La Iglesia
authors.forename: Carolin
authors.initials: C
authors.lastname: Lau
authors.forename: Eva
authors.initials: E
authors.lastname: Chi
authors.forename: Margaret
authors.initials: M
authors.lastname: Werner-Washburne
authors.forename: Scott
authors.initials: S
authors.lastname: Banta
authors.forename: Plamen
authors.initials: P
authors.lastname: Atanassov
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D042845:Laccase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D008958:Models, Molecular; D015202:Protein Engineering / Q000379:methods*; D017434:Protein Structure, Tertiary; D048372:Streptomyces coelicolor / Q000201:enzymology*; D013379:Substrate Specificity; D016335:Zinc Fingers
chemical_list: D004247:DNA; D042845:Laccase