title: Inhibition of peripheral blood neutrophil oxidative burst in periodontitis patients with a homeopathic medication Traumeel S.
abstract: BACKGROUND
  The anti-inflammatory effects of a homeopathic remedy, Traumeel S, have been observed in experimental and clinical studies; however, its antioxidant properties have not been elucidated. The aim of the present study was to evaluate the antioxidant effects of Traumeel S on peripheral blood neutrophils in patients with periodontitis.
  
  
  METHODS
  The study was performed using venous blood of 22 individuals with chronic periodontitis and 21 healthy subjects. The antioxidant effects of Traumeel S on the production of reactive oxygen species by unstimulated and stimulated with unopsonized E. coli neutrophils were investigated using luminol- and lucigenin-dependent chemiluminescence (CL).
  
  
  RESULTS
  Polymorphonuclear leukocytes of periodontitis patients produced higher levels (p<0.01) of light output of lucigenin-dependent chemiluminescence and significantly reduced (p<0.01) light output of luminol-dependent chemiluminescence than analogous cells of healthy subjects. Highly diluted (10⁻⁴ of the stem solution) Traumeel S significantly (by approximately 50%) reduced superoxide-induced oxidation of lucigenin by unstimulated and stimulated with unopsonized E. coli polymorphonuclear leukocytes of periodontitis patients and had a tendency to intensify luminol-dependent chemiluminescence. Preincubation of the unstimulated and stimulated with unopsonized E. coli polymorphonuclear leukocytes of healthy subjects with Traumeel S exerts no inhibitory action on the luminol- and lucigenin-dependent chemiluminescence of the above-mentioned cells.
  
  
  CONCLUSIONS
  This study indicates that Traumeel S may significantly reduce production of superoxide anion by unstimulated and stimulated peripheral blood polymorphonuclear neutrophils of periodontitis patients.
authors.affiliation: STOMATOLOGIJA Baltic Dental and Maxillofacial Journal, Kaunas, Lithuania.
authors.forename: Juozas
authors.initials: J
authors.lastname: Žilinskas
authors.forename: Jonas
authors.initials: J
authors.lastname: Žekonis
authors.forename: Gediminas
authors.initials: G
authors.lastname: Žekonis
authors.forename: Renata
authors.initials: R
authors.lastname: Šadzevičienė
authors.forename: Marija
authors.initials: M
authors.lastname: Sapragonienė
authors.forename: Justina
authors.initials: J
authors.lastname: Navickaitė
authors.forename: Ingrida
authors.initials: I
authors.lastname: Barzdžiukaitė
mesh_terms: D000166:Acridines / Q000378:metabolism; D000328:Adult; D005260:Female; D006705:Homeopathy; D006801:Humans; D008163:Luminescent Measurements; D008165:Luminol / Q000378:metabolism; D008297:Male; D008903:Minerals / Q000494:pharmacology*; D009504:Neutrophils / Q000187:drug effects* / Q000473:pathology*; D010518:Periodontitis / Q000097:blood* / Q000473:pathology*; D010936:Plant Extracts / Q000494:pharmacology*; D016897:Respiratory Burst / Q000187:drug effects*
chemical_list: D000166:Acridines; D008903:Minerals; D010936:Plant Extracts; C433370:Traumeel S; C033472:10,10'-dimethyl-9,9'-biacridinium; D008165:Luminol