title: Cytotoxicity and biocompatibility evaluation of a poly(magnesium acrylate) hydrogel synthesized for drug delivery.
abstract: We report the synthesis and characterization as well as cytotoxicity and biocompatibility studies of a poly(magnesium acrylate) hydrogel (PAMgA) developed for drug delivery applications. Two hydrogels with different mesh sizes, large and short, were synthesized (L-C PAMgA and S-C PAMgA). The hydrogels were characterized through swelling, FT-IR and DSC. Cytotoxicity in vitro was evaluated on cell line NIH-3T3 fibroblasts via direct contact and two indirect contact methods (MTT and flow citometry). Both PAMgA hydrogels exhibited low cytotoxicity with survival rates higher than 90%. To select their administration route, biocompatibility was evaluated after intraperitoneal, subcutaneous, and oral administration to mice of both hydrogels at different dose ranges. Swelling percentages obtained were 33.3 ± 4.2% and 166.7 ± 8.3% for L-C PAMgA and S-C PAMgA respectively, showing a great difference in both hydrogels. Among the administration routes assayed, the hydrogels were well tolerated after oral administration of a wide dose range (10-500 mg/kg), thereby indicating that both PAMgA hydrogels are excellent candidates for oral administration due to their in vitro biocompatibility and oral non-toxicity. These results together with the fact that their synthesis is simple and inexpensive make them good candidates for the design of oral drug delivery devices.
authors.affiliation: Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid, Spain.
authors.forename: Maha
authors.initials: M
authors.lastname: Cheddadi
authors.forename: Enrique
authors.initials: E
authors.lastname: López-Cabarcos
authors.forename: Karla
authors.initials: K
authors.lastname: Slowing
authors.forename: Emilia
authors.initials: E
authors.lastname: Barcia
authors.forename: Ana
authors.initials: A
authors.lastname: Fernández-Carballido
mesh_terms: D000179:Acrylates / Q000737:chemistry / Q000633:toxicity*; D000284:Administration, Oral; D000645:Ammonium Sulfate / Q000737:chemistry; D000818:Animals; D001672:Biocompatible Materials / Q000633:toxicity; D002452:Cell Count; D003603:Cytotoxins / Q000737:chemistry / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D016503:Drug Delivery Systems; D005562:Formazans / Q000378:metabolism; D020100:Hydrogels / Q000633:toxicity*; D007274:Injections, Intraperitoneal; D007279:Injections, Subcutaneous; D008276:Magnesium Hydroxide / Q000737:chemistry / Q000633:toxicity*; D008297:Male; D008422:Materials Testing; D051379:Mice; D041681:NIH 3T3 Cells; D011108:Polymers / Q000138:chemical synthesis; D013778:Tetrazolium Salts / Q000378:metabolism
chemical_list: D000179:Acrylates; D001672:Biocompatible Materials; D003603:Cytotoxins; D005562:Formazans; D020100:Hydrogels; D011108:Polymers; D013778:Tetrazolium Salts; C031276:ammonium peroxydisulfate; C079782:MTT formazan; D008276:Magnesium Hydroxide; D000645:Ammonium Sulfate