title: Synthesis of nanosilver using a vitamin C derivative and studies on radiation protection.
abstract: Silver nanoparticles were prepared from silver nitrate using a vitamin C derivative, 6-palmitoyl ascorbic acid-2-glucoside (PAsAG), via a sonochemical experiment. The resultant golden yellow solution that contained silver nanoparticle-PAsAG complex (SN-PAsAG) of about 5 nm particle sizes was explored for its potential to offer protection to DNA from γ-radiation-induced damages. The presence of SN-PAsAG during irradiation inhibited the disappearance of covalently closed circular (ccc) form of plasmid pBR322 with a dose modifying factor of 1.78. SN-PAsAG protected cellular DNA from radiation-induced damage as evident from comet assay study on mouse spleen cells, irradiated ex vivo. When orally administered with SN-PAsAG at 1 hour prior to whole-body radiation exposure, cellular DNA was found protected from radiation-induced strand breaks in various tissues (spleen cells, bone marrow cells, and blood leucocytes) of animals. Also, SN-PAsAG could enhance the rate of repair of cellular DNA in blood leucocytes and bone marrow cells when administered immediately after radiation exposure. The studies, under in vitro, ex vivo, and in vivo radiation exposure conditions, showed effective radiation protection.
authors.affiliation: Amala Cancer Research Centre, Thrissur, Kerala, India.
authors.forename: Dhanya K
authors.initials: DK
authors.lastname: Chandrasekharan
authors.forename: Pawan K
authors.initials: PK
authors.lastname: Khanna
authors.forename: Tsutomu V
authors.initials: TV
authors.lastname: Kagiya
authors.forename: Cherupally Krishnan Krishnan
authors.initials: CK
authors.lastname: Nair
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D004247:DNA / Q000187:drug effects* / Q000528:radiation effects; D004249:DNA Damage / Q000187:drug effects; D004260:DNA Repair / Q000187:drug effects; D005720:Gamma Rays / Q000009:adverse effects; D005960:Glucosides / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D053768:Metal Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry*; D051379:Mice; D010957:Plasmids / Q000187:drug effects; D011835:Radiation Protection; D011837:Radiation-Protective Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D012835:Silver Nitrate / Q000737:chemistry*; D014916:Whole-Body Irradiation
chemical_list: C543571:6-palmitoyl ascorbic acid-2-glucoside; D005960:Glucosides; D011837:Radiation-Protective Agents; D004247:DNA; D012835:Silver Nitrate; D001205:Ascorbic Acid