title: Evaluation of sperm proteins in infertile men: a proteomic approach.
abstract: In this study, the sperm protein profile was compared between fertile and infertile men using 2-dimensional gel electrophoresis, liquid chromatography mass spectrometer analysis, and matrix-assisted laser desorption ionization-time-of-flight-mass spectrometry. Four unique proteins, semenogelin II precursor, prolactin-induced protein, clusterin isoform 1, and prostate-specific antigen isoform 1 preproprotein, were predominantly present in the semen of healthy men; however, semenogelin II precursor and clusterin isoform 1 were not seen in the semen of infertile men, suggesting unique differences in the spermatozoa protein profiles of fertile and infertile men.
authors.affiliation: Molecular Biotechnology Core Laboratory, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio, USA.
authors.forename: Stetson
authors.initials: S
authors.lastname: Thacker
authors.forename: Satya P
authors.initials: SP
authors.lastname: Yadav
authors.forename: Rakesh K
authors.initials: RK
authors.lastname: Sharma
authors.forename: Anthony
authors.initials: A
authors.lastname: Kashou
authors.forename: Belinda
authors.initials: B
authors.lastname: Willard
authors.forename: Dongmei
authors.initials: D
authors.lastname: Zhang
authors.forename: Ashok
authors.initials: A
authors.lastname: Agarwal
mesh_terms: D000595:Amino Acid Sequence; D015415:Biomarkers / Q000032:analysis; D002352:Carrier Proteins / Q000032:analysis; D016022:Case-Control Studies; D056148:Chromatography, Reverse-Phase; D051152:Clusterin / Q000032:analysis; D015180:Electrophoresis, Gel, Two-Dimensional; D005298:Fertility; D006023:Glycoproteins / Q000032:analysis; D006801:Humans; D007248:Infertility, Male / Q000378:metabolism* / Q000503:physiopathology; D008297:Male; D026901:Membrane Transport Proteins; D008970:Molecular Weight; D009820:Ohio; D010865:Pilot Projects; D017430:Prostate-Specific Antigen / Q000032:analysis; D040901:Proteomics / Q000379:methods; D029589:Seminal Plasma Proteins / Q000032:analysis* / Q000737:chemistry; D029607:Seminal Vesicle Secretory Proteins / Q000032:analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013094:Spermatozoa / Q000737:chemistry*
chemical_list: D015415:Biomarkers; C495242:CLU protein, human; D002352:Carrier Proteins; D051152:Clusterin; D006023:Glycoproteins; D026901:Membrane Transport Proteins; C488892:PIP protein, human; D029589:Seminal Plasma Proteins; D029607:Seminal Vesicle Secretory Proteins; C424482:seminal vesicle-specific antigen; D017430:Prostate-Specific Antigen