title: Indole-3-carbinol inhibits prostate cancer cell migration via degradation of beta-catenin.
abstract: We determined whether indole-3-carbinol (I3C) could affect DU145 human prostate carcinoma cell migration to prevent the development and progression of prostate cancer. Although previous studies have shown anticancer properties of I3C in various cancer cell lines, it has not been determined how I3C regulates epidermal growth factor (EGF)-induced migration and related signaling pathways. DU145 cells were treated with I3C (100 microM) in the absence or presence of EGF (10 ng/ml). Our results showed that I3C significantly inhibited DU145 cell migration with and without EGF stimulation. It has been reported that the beta-catenin signaling pathway controls androgen receptor (AR)-mediated prostate cancer progression, which plays a key role in the metastasis of prostate cancer. Western blot analysis demonstrated that I3C led to the phosphorylation of beta-catenin and subsequent degradation of beta-catenin in the absence and presence of EGF. In contrast, I3C did not have any effect on the expression of beta-catenin mRNA. From these results, we suggest that I3C inhibits EGF (dependent or independent)-induced DU145 cell migration through beta-catenin degradation.
authors.affiliation: Department of Biochemistry, Chung-Ang University College of Medicine, Seoul, Korea.
authors.forename: Yun-Mi
authors.initials: YM
authors.lastname: Jeong
authors.forename: Hailan
authors.initials: H
authors.lastname: Li
authors.forename: Su Yeon
authors.initials: SY
authors.lastname: Kim
authors.forename: Hye-Young
authors.initials: HY
authors.lastname: Yun
authors.forename: Kwang Jin
authors.initials: KJ
authors.lastname: Baek
authors.forename: Nyoun Soo
authors.initials: NS
authors.lastname: Kwon
authors.forename: Soon Chul
authors.initials: SC
authors.lastname: Myung
authors.forename: Dong-Seok
authors.initials: DS
authors.lastname: Kim
mesh_terms: D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D002465:Cell Movement / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000494:pharmacology; D006801:Humans; D007211:Indoles / Q000494:pharmacology*; D008297:Male; D010766:Phosphorylation; D011471:Prostatic Neoplasms / Q000188:drug therapy* / Q000473:pathology; D051176:beta Catenin / Q000378:metabolism*
chemical_list: D016588:Anticarcinogenic Agents; D007211:Indoles; D051176:beta Catenin; D004815:Epidermal Growth Factor; C016517:indole-3-carbinol