title: Assessment of cardiovascular fibrosis using novel fluorescent probes.
abstract: Cardiovascular fibrosis resulted from pressure overload or ischemia could alter myocardial stiffness and lead to ventricular dysfunction. Fluorescently labeled collagen-binding protein CNA 35, derived from the surface component of Staphylococcus aureus, and a novel synthetic biphenylalanine containing peptide are applied to stain fibrosis associated collagen and myocytes, respectively. Detailed pathological characteristics of cardiovascular fibrosis could be identified clearly in 2 hours. This staining pair requires only simple staining and brief washing, generating less than 10 ml of waste. The image information collected by this novel fluorescent staining pair is compatible with it collected by the traditional Masson's Trichrome and Picrosirius Red staining which are widely used to stain cardiovascular fibrosis and isolated cells.
authors.affiliation: Cardiovascular Research Center, Mount Sinai School of Medicine, New York, New York, United States of America.
authors.forename: Jiqiu
authors.initials: J
authors.lastname: Chen
authors.forename: Seung Koo
authors.initials: SK
authors.lastname: Lee
authors.forename: Wael R
authors.initials: WR
authors.lastname: Abd-Elgaliel
authors.forename: Lifan
authors.initials: L
authors.lastname: Liang
authors.forename: Elisa-Yaniz
authors.initials: EY
authors.lastname: Galende
authors.forename: Roger J
authors.initials: RJ
authors.lastname: Hajjar
authors.forename: Ching-Hsuan
authors.initials: CH
authors.lastname: Tung
mesh_terms: D000818:Animals; D002318:Cardiovascular Diseases / Q000473:pathology*; D005355:Fibrosis; D005456:Fluorescent Dyes; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013194:Staining and Labeling
chemical_list: D005456:Fluorescent Dyes