title: Coupled expression of dipeptidyl peptidase-IV and fibroblast activation protein-α in transformed astrocytic cells.
abstract: Dipeptidyl peptidase-IV (DPP-IV) and fibroblast activation protein-α (FAP) are speculated to participate in the regulation of multiple biological processes, because of their unique enzymatic activity, as well as by non-hydrolytic molecular interactions. At present, the role of DPP-IV and FAP in the development and progression of various types of tumors, including glioblastoma, is intensively studied, and their functional crosstalk is hypothesized. In this article, we describe the correlative expression of DPP-IV and FAP mRNA in primary cell cultures derived from human glioblastoma and associated expression dynamics of both molecules in astrocytoma cell lines depending on culture conditions. Although the molecular mechanisms of DPP-IV and FAP co-regulations remain unclear, uncoupled expression of transgenic DPP-IV and the endogenous FAP suggests that it occurs rather at the transcriptional than at the posttranscriptional level. Understanding of the expressional and functional coordinations of DPP-IV and FAP may help clarify the mechanisms of biological roles of both molecules in transformed astrocytic cells.
authors.affiliation: Institute of Biochemistry and Experimental Oncology of the 1st Faculty of Medicine, Charles University in Prague, U Nemocnice 5, Prague 2, 12853, Czech Republic.
authors.forename: Eva
authors.initials: E
authors.lastname: Balaziova
authors.forename: Petr
authors.initials: P
authors.lastname: Busek
authors.forename: Jarmila
authors.initials: J
authors.lastname: Stremenova
authors.forename: Lucie
authors.initials: L
authors.lastname: Sromova
authors.forename: Evzen
authors.initials: E
authors.lastname: Krepela
authors.forename: Libuse
authors.initials: L
authors.lastname: Lizcova
authors.forename: Aleksi
authors.initials: A
authors.lastname: Sedo
mesh_terms: D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002457:Cell Extracts / Q000737:chemistry; D002461:Cell Line, Transformed; D018819:Dipeptidyl Peptidase 4 / Q000235:genetics / Q000378:metabolism*; D010450:Endopeptidases; D057075:Enzyme Assays; D018093:Gelatinases / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D009457:Neuroglia / Q000201:enzymology / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*; D012697:Serine Endopeptidases / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic; D000099167:Fibroblast Activation Protein Alpha
chemical_list: D002457:Cell Extracts; D018819:Dipeptidyl Peptidase 4; D010450:Endopeptidases; D018093:Gelatinases; D008565:Membrane Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D012697:Serine Endopeptidases; D000099167:Fibroblast Activation Protein Alpha