title: Effective compounds group of Mongolian prescriptions BAIMAI-SAN protect against peripheral neuropathy in lower limbs of rats through neuro protective effect.
abstract: BACKGROUND
  BAIMAI-SAN prescription is a famous Chinese minority complex prescription used for curing neuropathy.
  
  
  METHODS
  The Effective Compounds Groups of BAIMAI-SAN (ECGBM) is determined by high through-put screening, and it includes picroside II, verbascose, taurine and ellagic acid and borneol. To research the potential protective effect of ECGBM on the function of peripheral neuropathy, diabetic rats with peripheral neuropathy were induced by streptozotocin and treated with ECGBM (0.1, 0.3, 0.9 mg/kg/day i.g.) for 75 days. Primary cortical neuronal cultures were subjected to high d-glucitol, and treated with ECGBM prophylactically.
  
  
  RESULTS
  The administration resulted in reductions in speed of sciatic motor nerve conduction velocity (MNCV), sensory nerve conduction velocity (SNCV) and response speed to pain in the sciatic nerve fiber. Data from primary cortical neuronal cultures experiments indicated that neuronal survival rates were increased, and LDH release was decreased and the loss of neurite length was alleviated in ECGBM group.
  
  
  CONCLUSIONS
  It is first report that ECGBM could protect the peripheral neuron in diabetic rat in vivo and in vitro. This activity may be associated with the neuron protective effect.
authors.affiliation: National Research Center for Minority Medicine, Minzu University of China, Beijing 100081, PR China.
authors.forename: Qing-Shan
authors.initials: QS
authors.lastname: Liu
authors.forename: Zong-Ran
authors.initials: ZR
authors.lastname: Pang
authors.forename: Rui
authors.initials: R
authors.lastname: Liu
authors.forename: Guo-Rong
authors.initials: GR
authors.lastname: He
authors.forename: Jian
authors.initials: J
authors.lastname: Cui
authors.forename: Xiao-ying
authors.initials: XY
authors.lastname: Yin
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology / Q000187:drug effects; D018929:Cell Culture Techniques; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D048909:Diabetes Complications / Q000188:drug therapy* / Q000503:physiopathology; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D005260:Female; D006614:Hindlimb; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D008519:Medicine, Traditional; D008986:Mongolia; D009431:Neural Conduction / Q000187:drug effects* / Q000502:physiology; D016501:Neurites / Q000187:drug effects; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology / Q000502:physiology; D018696:Neuroprotective Agents / Q000032:analysis / Q000494:pharmacology / Q000627:therapeutic use*; D010146:Pain / Q000209:etiology / Q000503:physiopathology; D010523:Peripheral Nervous System Diseases / Q000188:drug therapy* / Q000209:etiology / Q000503:physiopathology; D008517:Phytotherapy; D010946:Plants, Medicinal; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000187:drug effects / Q000503:physiopathology; D011984:Sensory Receptor Cells / Q000187:drug effects / Q000502:physiology; D013012:Sorbitol
chemical_list: D004365:Drugs, Chinese Herbal; D018696:Neuroprotective Agents; D013012:Sorbitol; D007770:L-Lactate Dehydrogenase