title: Genetic variations in fetal and maternal tumor necrosis factor-α and interleukin 10: is there an association with preterm birth or periventricular leucomalacia?
abstract: OBJECTIVE
  The aim of the study was to identify whether tumor necrosis factor-α (TNF-α) (-308) and interleukin (IL)-10 (-1082; -819) genotypes were associated with preterm delivery and cystic periventricular leucomalacia (PVL).
  
  
  METHODS
  Venous blood, buccal swabs or cord blood were collected from mother/child pairs with infants born at term (200) or preterm (106) in the presence and absence of neonatal PVL and of premature infants with PVL (7). Extracted genomic DNA served as template for determination of IL-10 (-1082), IL-10 (-819) and TNF-α (-308) genotypes by allele-specific PCR.
  
  
  RESULTS
  No significant difference was observed in the frequencies of IL-10 (-1082), IL-10 (-819) and TNF-α (-308) genotypes in mothers or in children of term versus preterm deliveries with or without PVL.
  
  
  CONCLUSIONS
  Maternal and infant IL-10 (-1082, -819) and TNF-α (-308) genotypes are not indicative for an increased risk of preterm birth or the development of PVL in premature newborns.
authors.affiliation: Center for Medical Research, Medical University of Graz, Graz, Austria.
authors.forename: M
authors.initials: M
authors.lastname: Nuk
authors.forename: K
authors.initials: K
authors.lastname: Orendi
authors.forename: S
authors.initials: S
authors.lastname: Rosenberger
authors.forename: A
authors.initials: A
authors.lastname: Griesbacher
authors.forename: M
authors.initials: M
authors.lastname: Holzapfel-Bauer
authors.forename: B
authors.initials: B
authors.lastname: Resch
authors.forename: U
authors.initials: U
authors.lastname: Lang
authors.forename: B
authors.initials: B
authors.lastname: Pertl
mesh_terms: D000293:Adolescent; D000328:Adult; D005260:Female; D014644:Genetic Variation; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature / Q000097:blood*; D016753:Interleukin-10 / Q000097:blood / Q000235:genetics*; D007969:Leukomalacia, Periventricular / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011248:Pregnancy Complications; D047928:Premature Birth / Q000235:genetics*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000235:genetics*; D055815:Young Adult
chemical_list: D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10