title: Extenders containing dimethylformamide associated or not with glycerol are ineffective for ovine sperm cryopreservation.
abstract: The study aimed at testing the effectiveness of dimethylformamide, alone or combined with glycerol, as cryoprotectant for freezing ram semen. Ejaculates from nine rams were cryopreserved in Tris-based extenders, containing 5% of glycerol, association of dimethylformamide with glycerol, in four proportions achieving 5% of cryoprotectors in the media and pure dimethylformamide (2, 3, 4 and 5%) in replacement to glycerol. The samples were diluted to 100 × 10(6) sptz/ml and stored in 0.25-ml straws in liquid nitrogen. After thawing (37 °C for 30 s), motility was preserved better by the extender containing 5% of glycerol (p < 0.05). The extenders containing pure dimethylformamide, or more than 2% in combination with glycerol, provided sperm motilities close to zero. Plasma and acrosomal membrane integrity were preserved better (p < 0.05) in the extender containing 5% glycerol. It can be concluded that dimethylformamide, alone or combined with glycerol, has no beneficial effects on ovine semen cryopreservation.
authors.affiliation: Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: V S
authors.initials: VS
authors.lastname: Moustacas
authors.forename: B C
authors.initials: BC
authors.lastname: Cruz
authors.forename: F C
authors.initials: FC
authors.lastname: Varago
authors.forename: D A
authors.initials: DA
authors.lastname: Miranda
authors.forename: P G
authors.initials: PG
authors.lastname: Lage
authors.forename: M
authors.initials: M
authors.lastname: Henry
mesh_terms: D000818:Animals; D004126:Dimethylformamide / Q000494:pharmacology*; D005990:Glycerol / Q000494:pharmacology*; D008297:Male; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D012756:Sheep / Q000502:physiology*; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects*
chemical_list: D004126:Dimethylformamide; D005990:Glycerol