title: Identification of a 27.8 kDa protein from flounder gill cells involved in lymphocystis disease virus binding and infection.
abstract: In vitro, lymphocystis disease virus (LCDV) infection of flounder gill (FG) cell cultures causes obvious cytopathic effect (CPE). We describe attempts to isolate and characterize the LCDV-binding molecule(s) on the plasma membrane of FG cells that were responsible for virus entry. The results showed that the co-immunoprecipitation assay detected a 27.8 kDa molecule from FG cells that bound to LCDV. In a blocking ELISA, pre-incubation of FG cell membrane proteins with the specific antiserum developed against the 27.8 kDa protein could block LCDV binding. Similarly, antiserum against 27.8 kDa protein could also inhibit LCDV infection of FG cells in vitro. Mass spectrometric analysis established that the 27.8 kDa protein and beta-actin had a strong association. These results strongly supported the possibility that the 27.8 kDa protein was the putative receptor specific for LCDV infection of FG cells.
authors.affiliation: Laboratory of Pathology and Immunology of Aquatic Animals, Ocean University of China, 5 Yushan Road, Qingdao 266003, PR China.
authors.forename: Mu
authors.initials: M
authors.lastname: Wang
authors.forename: Xiu-Zhen
authors.initials: XZ
authors.lastname: Sheng
authors.forename: Jing
authors.initials: J
authors.lastname: Xing
authors.forename: Xiao-Qian
authors.initials: XQ
authors.lastname: Tang
authors.forename: Wen-Bin
authors.initials: WB
authors.lastname: Zhan
mesh_terms: D000818:Animals; D002460:Cell Line; D004266:DNA Virus Infections / Q000378:metabolism / Q000662:veterinary*; D005393:Fish Diseases / Q000821:virology*; D005432:Flounder / Q000502:physiology*; D015967:Gene Expression Regulation, Viral / Q000502:physiology; D005880:Gills / Q000166:cytology*; D007497:Iridoviridae / Q000378:metabolism*; D011485:Protein Binding; D014764:Viral Proteins / Q000378:metabolism; D053585:Virus Attachment; D014779:Virus Replication
chemical_list: D014764:Viral Proteins