title: [Chemical constituents of rhizoma imperatae and their anti-complementary activity].
abstract: OBJECTIVE
  To study the chemical constituents of Rhizoma Imperatae and their anti-complementary activity.
  
  
  METHODS
  By the hemolysis test, the petroleum extraction, ethyl acetate extraction, n-butanol extraction and the water extraction was tested for anti-complementary activity. Compounds were isolated and purified by silica gel column chromatography, Sephadex LH-20 and reversed-phase column chromatography. The structures were identified by the various spectroscopic data of ESI-MS, 1H-NMR, 13C-NMR. The compounds were evaluated for anti-complementary activity in vitro.
  
  
  RESULTS
  The petroleum extraction, ethyl acetate extraction showed significant anti-complementary activity. Ten compounds were isolated from the petroleum and EtOAc soluble fractions and identified as cylindrin (1), arundoin (2), friedelin (3), beta-sitosterol (4), siderin (5), ethyl p-hydroxybenzoate (6), 5-methoxyflavone (7), vanillic acid (8), trans-p-coumaric acid (9), 5-hydroxymethylfurfural (10).
  
  
  CONCLUSIONS
  Compounds 6, 7, 8, and 10 are isolated from the genus for the first time, and compounds 3, 8 and 9 inhibited the complement system towards the classical pathway.
authors.affiliation: Key Laboratory of Modern Preparation of TCM, Nanchang 330004, China.
authors.forename: Li-Na
authors.initials: LN
authors.lastname: Fu
authors.forename: Lan-Ying
authors.initials: LY
authors.lastname: Chen
authors.forename: Rong-Hua
authors.initials: RH
authors.lastname: Liu
authors.forename: Dao-Feng
authors.initials: DF
authors.lastname: Chen
mesh_terms: D000818:Animals; D003169:Complement Inactivator Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D003171:Complement Pathway, Classical / Q000187:drug effects; D005260:Female; D047309:Flavones / Q000737:chemistry / Q000302:isolation & purification; D006168:Guinea Pigs; D020128:Inhibitory Concentration 50; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D010226:Parabens / Q000737:chemistry / Q000302:isolation & purification; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry*; D006109:Poaceae / Q000737:chemistry*; D027343:Rhizome / Q000737:chemistry; D014641:Vanillic Acid / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: C503867:3',5'-dimethoxyflavone; D003169:Complement Inactivator Proteins; D047309:Flavones; D010226:Parabens; D010936:Plant Extracts; C012313:ethyl-p-hydroxybenzoate; D014641:Vanillic Acid