title: Neuroprotective mechanisms of SMTP-7 in cerebral infarction model in mice.
abstract: Reactive oxygen species (ROS) formation has been found to induce the brain damage following stroke-like events. The aim of the present study was to investigate the effect of Stachybotrys microspora triprenyl phenol-7 (SMTP-7) on the generation of ROS in ischemia-induced cerebral infarction model and in vitro lipid peroxidation. We used immunohistochemistry and real-time reverse-transcription PCR for ex vivo evaluation and thiobarbituric acid-reactive substance reagent assay for in vitro evaluation. We demonstrated that SMTP-7 did not induce enhancement of 4-hydroxynonenal or neutrophil cytosolic factor 2 like t-PA administration at 3 h after ischemia ex vivo and reduce lipid peroxidation in vitro. This compound is the first low molecular weight compound with triplet activities of thrombolytic, anti-inflammatory, and antioxidant activities. We theorized that SMTP-7 is among the pharmacological agents that reduce ROS formation and have been found to limit the extent of brain damage following stroke-like events.
authors.affiliation: Department of Pharmacology, School of Pharmacy, Showa University, Hatanodai, Shinagawa-ku, Tokyo, Japan. kshibata@pharm.showa-u.ac.jp
authors.forename: Keita
authors.initials: K
authors.lastname: Shibata
authors.forename: Terumasa
authors.initials: T
authors.lastname: Hashimoto
authors.forename: Koji
authors.initials: K
authors.lastname: Nobe
authors.forename: Keiji
authors.initials: K
authors.lastname: Hasumi
authors.forename: Kazuo
authors.initials: K
authors.lastname: Honda
mesh_terms: D000447:Aldehydes / Q000378:metabolism; D000818:Animals; D000983:Antipyrine / Q000031:analogs & derivatives / Q000494:pharmacology; D001578:Benzopyrans / Q000494:pharmacology* / Q000627:therapeutic use; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D002544:Cerebral Infarction / Q000188:drug therapy / Q000378:metabolism / Q000517:prevention & control*; D004195:Disease Models, Animal; D000077553:Edaravone; D005296:Ferrous Compounds / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000494:pharmacology / Q000627:therapeutic use; D015870:Gene Expression / Q000187:drug effects / Q000235:genetics; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D019255:NADPH Oxidases / Q000235:genetics; D018696:Neuroprotective Agents / Q000494:pharmacology* / Q000627:therapeutic use; D011760:Pyrrolidinones / Q000494:pharmacology* / Q000627:therapeutic use; D017382:Reactive Oxygen Species / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D010959:Tissue Plasminogen Activator / Q000494:pharmacology / Q000627:therapeutic use
chemical_list: D000447:Aldehydes; D001578:Benzopyrans; D005296:Ferrous Compounds; D016166:Free Radical Scavengers; D018696:Neuroprotective Agents; D011760:Pyrrolidinones; D017382:Reactive Oxygen Species; C409191:SMTP 7; D017392:Thiobarbituric Acid Reactive Substances; D019255:NADPH Oxidases; C511584:Ncf2 protein, mouse; D010959:Tissue Plasminogen Activator; C027576:4-hydroxy-2-nonenal; D000077553:Edaravone; D000983:Antipyrine