title: DNA diagnostics with a bacterial reporter probe.
abstract: A DNA diagnostic system has been developed by using a bacterial reporter probe. DNA hybridization-driven magnetic isolation of E. coli, followed by exponential bacterial growth and fluorescent protein expression, allows the translation of a target binding event into a vastly amplified signal and a highly sensitive/selective detection platform.
authors.affiliation: Department of Polymer Science and Engineering, School of Chemistry and Chemical Engineering, State Key Laboratory of Coordination Chemistry, Nanjing University, Nanjing 210093, China.
authors.forename: Yishu
authors.initials: Y
authors.lastname: Yan
authors.forename: Wei
authors.initials: W
authors.lastname: Sha
authors.forename: Jingjing
authors.initials: J
authors.lastname: Sun
authors.forename: Xin
authors.initials: X
authors.lastname: Shu
authors.forename: Sha
authors.initials: S
authors.lastname: Sun
authors.forename: Jin
authors.initials: J
authors.lastname: Zhu
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D015342:DNA Probes / Q000235:genetics*; D004926:Escherichia coli / Q000302:isolation & purification*; D008280:Magnetics; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009693:Nucleic Acid Hybridization
chemical_list: D015342:DNA Probes; D004247:DNA