title: Evaluation of anti-inflammatory activity of derivatives from aerial parts of Baccharis uncinella.
abstract: BACKGROUND
  Species of Baccharis exhibit antibiotic, antiseptic, and wound-healing properties, and have been used in the traditional medicine of South America for the treatment of inflammation, headaches, diabetes, and hepatobiliary disorders.
  
  
  OBJECTIVE
  To investigate the anti-inflammatory activity of organic phases from EtOH extract of the aerial parts of Baccharis uncinella DC (Asteraceae).
  
  
  METHODS
  The crude EtOH extract from the aerial parts of B. uncinella was subjected to partition procedures and the corresponding CH(2)Cl(2) and EtOAc phases were subjected to several chromatographic separation procedures. Thus, these phases and their purified compounds were assayed for evaluation of anti-inflammatory activity.
  
  
  RESULTS
  The CH(2)Cl(2) phase from EtOH extract from B. uncinella contained two triterpenoids (oleanolic and ursolic acids) and one flavonoid (pectolinaringenin), whereas the respective EtOAc phase showed to be composed mainly by two phenylpropanoid derivatives (caffeic and ferulic acids). The CH(2)Cl(2) and EtOAc phases as well as their isolated compounds exhibited anti-inflammatory effects against inflammatory reactions induced by phospholipase A2 (from Crotalus durissus terrificus venom) and by carrageenan.
  
  
  CONCLUSIONS
  The results suggested that the components obtained from partition phases of EtOH extract of B. uncinella could represent lead molecules for the development of anti-inflammatory agents. Additionally, the results confirmed the use of Baccharis genus in the traditional medicine of South America for the treatment of inflammation and other heath disorders. To date, the present work describes for the first time the anti-inflammatory effects of compounds isolated from B. uncinella.
authors.affiliation: Departamento de Ciências Exatas e da Terra, Universidade Federal de São Paulo, Diadema, SP, Brazil.
authors.forename: Cynthia A
authors.initials: CA
authors.lastname: Zalewski
authors.forename: Luiz Felipe D
authors.initials: LF
authors.lastname: Passero
authors.forename: Alexis S R B
authors.initials: AS
authors.lastname: Melo
authors.forename: Carlos Eduardo P
authors.initials: CE
authors.lastname: Corbett
authors.forename: Márcia D
authors.initials: MD
authors.lastname: Laurenti
authors.forename: Marcos H
authors.initials: MH
authors.lastname: Toyama
authors.forename: Daniela O
authors.initials: DO
authors.lastname: Toyama
authors.forename: Paulete
authors.initials: P
authors.lastname: Romoff
authors.forename: Oriana A
authors.initials: OA
authors.lastname: Fávero
authors.forename: João Henrique G
authors.initials: JH
authors.lastname: Lago
mesh_terms: D000085:Acetates / Q000737:chemistry; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D031175:Baccharis / Q000737:chemistry*; D004353:Drug Evaluation, Preclinical; D000431:Ethanol / Q000737:chemistry; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy*; D008297:Male; D008519:Medicine, Traditional / Q000379:methods; D008752:Methylene Chloride / Q000737:chemistry; D054497:Phospholipases A2, Secretory / Q000037:antagonists & inhibitors*; D008517:Phytotherapy / Q000379:methods*; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000085:Acetates; D000893:Anti-Inflammatory Agents; D010936:Plant Extracts; D000431:Ethanol; D008752:Methylene Chloride; C007650:ethyl acetate; D054497:Phospholipases A2, Secretory