title: Analysis of expressed sequence tags (ESTs) and gene expression changes under different growth conditions for the ciliate Anophryoides haemophila, the causative agent of bumper car disease in the American lobster (Homarus americanus).
abstract: The scuticociliate Anophryoides haemophila, causes bumper car disease in American lobster (Homarus americanus) in commercial holding facilities in Atlantic Canada. While the parasite has been recognized since the 1970s and much has been learned about its biology, minimal molecular characterization exists. With genome consortiums turning to model organisms like the ciliates Tetrahymena and Paramecium, the amount of relevant sequence data available has made sequence surveys more attractive for gene discovery in related ciliates. We sequenced 9984 expressed sequence tags (ESTs) from a non-normalized A. haemophila cDNA library to characterize gene expression patterns, functional gene distribution and to discover novel genes related to the parasitic life history. The A. haemophila ESTs were grouped into 843 clusters and singletons with 658 EST clusters having identifiable homologs, while 159 ESTs were unique and had no similarity to any sequences in the public databases. Not unexpectedly, about 67% of the A. haemophila ESTs have similarity to annotated and hypothetical genes from the related oligohymenophorean ciliate, Tetrahymena. Numerous cysteine proteases, hypothetical proteins and novel sequences possess putative secretory signal peptides suggesting that they may contribute to the pathogenesis of bumper car disease in lobster. Real time RT-qPCR analysis of cathepsin L and two homologs of cathepsin B did not show any changes in gene expression under varying in vitro growth conditions or during a modified-in vivo infection which may be suggestive of the opportunistic life history strategy of this ciliate.
authors.affiliation: AVC Lobster Science Centre, Atlantic Veterinary College, University of Prince Edward Island, 550 University Ave., Charlottetown, Prince Edward Island, Canada C1A 4P3. aacorn@upei.ca
authors.forename: Adam R
authors.initials: AR
authors.lastname: Acorn
authors.forename: K Fraser
authors.initials: KF
authors.lastname: Clark
authors.forename: Sarah
authors.initials: S
authors.lastname: Jones
authors.forename: Béatrice M
authors.initials: BM
authors.lastname: Després
authors.forename: Sarah
authors.initials: S
authors.lastname: Munro
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Cawthorn
authors.forename: Spencer J
authors.initials: SJ
authors.lastname: Greenwood
mesh_terms: D000818:Animals; D002401:Cathepsin B / Q000235:genetics; D056668:Cathepsin L / Q000235:genetics; D016798:Ciliophora / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity; D016770:Ciliophora Infections / Q000235:genetics / Q000662:veterinary*; D020224:Expressed Sequence Tags / Q000737:chemistry; D015870:Gene Expression; D020869:Gene Expression Profiling; D015723:Gene Library; D006790:Host-Parasite Interactions / Q000235:genetics*; D008297:Male; D008121:Nephropidae / Q000469:parasitology*; D009656:North America; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D002401:Cathepsin B; D056668:Cathepsin L