title: In-vivo effect of the combination of tnf and adriamycin against a human breast cell-line expressing the mdr-phenotype.
abstract: The combined effect of tumor necrosis factor alpha (TNF) and adriamycin (ADR) on tumor cell killing was investigated in vivo. The human breast adenocarcinoma ADR-resistant MDA/ADR cells found to be resistant to in vitro TNF lysis and with an apparent index of resistance to ADR of 23 have been used. Treatment of MDA/ADR subcutaneous tumor bearing mice with PBS, TNF, ADR or the combination of TNF/ADR indicate that the combination of TNF and ADR treatment leads to a significant decrease in tumor growth while treatment with TNF was inactive and treatment with ADR was moderately active. Tumor cells were collected from mice treated with TNF/ADR combination and analysed by PCR for MnSOD and TNF ne expression. No induction of the expression of these genes, known to be involved in the regulation of TNF-induced cell killing, was noted following TNF/ADR combination treatment, suggesting that their products were not involved in the observed resistance.
authors.affiliation: INST GUSTAVE ROUSSY,INSERM,CJF 94-11,IMMUNOL LAB,F-94805 VILLEJUIF,FRANCE. INST GUSTAVE ROUSSY,PHARMACOL CLIN & MOLEC LAB,VILLEJUIF,FRANCE. INST GUSTAVE ROUSSY,MOLEC PHARMACOL LAB,CNRS,URA 147,VILLEJUIF,FRANCE.
authors.forename: A
authors.initials: A
authors.lastname: Zyad
authors.forename: Z
authors.initials: Z
authors.lastname: Cai
authors.forename: J
authors.initials: J
authors.lastname: Morizet
authors.forename: L
authors.initials: L
authors.lastname: Legres
authors.forename: J
authors.initials: J
authors.lastname: Benard
authors.forename: S
authors.initials: S
authors.lastname: Chouaib