title: Proteomic approach for the detection of chicken mechanically recovered meat.
abstract: Mechanically recovered meat is cheaper than raw meat and thus has been incorporated into many meat-derived products. EU regulations exclude mechanically recovered meat from the definition of meat; as a consequence analytical procedures are needed to differentiate it from hand-deboned meat. The present pilot study has utilized a proteomic approach to find potential markers for the detection of chicken mechanically recovered meat. Intact proteins were extracted from raw meat and then analyzed with OFF-GEL electrophoresis followed by SDS-PAGE and identification of potential markers by nano-LC-MS/MS. It was shown that it is possible to extract, separate and identify key proteins from processed meat material. Potential chicken mechanically recovered meat markers--hemoglobin subunits and those similar to myosin-binding protein C were also identified.
authors.affiliation: School of Biological Sciences, Royal Holloway University of London, Egham, Surrey, TW20 0EX, UK.
authors.forename: I
authors.initials: I
authors.lastname: Surowiec
authors.forename: K M
authors.initials: KM
authors.lastname: Koistinen
authors.forename: P D
authors.initials: PD
authors.lastname: Fraser
authors.forename: P M
authors.initials: PM
authors.lastname: Bramley
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis / Q000737:chemistry; D002352:Carrier Proteins / Q000302:isolation & purification; D002645:Chickens; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005504:Food Analysis / Q000379:methods; D005511:Food Handling / Q000379:methods*; D055554:Hemoglobin Subunits / Q000302:isolation & purification; D013058:Mass Spectrometry; D008460:Meat / Q000032:analysis*; D010865:Pilot Projects; D040901:Proteomics / Q000379:methods*; D000099163:Myosin Binding Protein C
chemical_list: D015415:Biomarkers; D002352:Carrier Proteins; D055554:Hemoglobin Subunits; D000099163:Myosin Binding Protein C