title: Identification of a polymeric β-cyclodextrin-binding peptide from a phage-displayed peptide library.
abstract: In this work, a phage-displayed peptide library was applied to identification of β-cyclodextrin (CD)-binding peptide tag, capable of being combined to target peptides or proteins in a homogeneous way by established methods such as peptide synthesis and the recombinant DNA technique. Four enriched sequences were obtained after five rounds of biopanning against polymeric β-CD beads. One of the sequences showed high binding affinity to β-CD beads with a dissociation constant of approximately 7×10(-6)M. The β-CD-binding sequence was used for immobilization of a hepatitis C virus (HCV) antigenic peptide on β-CD beads. The functionalized β-CD beads were successfully used for immunoassay of anti-HCV antibody with a detection limit of 1 ng. These results demonstrate that the identified peptide sequence has the potential of being used as an affinity tag to β-CD-containing surfaces.
authors.affiliation: Department of Chemistry, Gangneung-Wonju National University, Gangneung 210-702, Republic of Korea.
authors.forename: Boram
authors.initials: B
authors.lastname: Kang
authors.forename: Suk-Jung
authors.initials: SJ
authors.lastname: Choi
mesh_terms: D019793:Fluorescein / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D018937:Hepatitis C Antibodies / Q000032:analysis; D019151:Peptide Library; D011485:Protein Binding; D014760:Viral Fusion Proteins / Q000737:chemistry; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D018937:Hepatitis C Antibodies; D019151:Peptide Library; D014760:Viral Fusion Proteins; D047392:beta-Cyclodextrins; C031215:betadex; D019793:Fluorescein