title: Overexpression of Grb2-associated binder 2 in human lung cancer.
abstract: OBJECTIVE
  Grb2-associated binder 2 (Gab2), a member of the family of Gab scaffolding adaptors, transmits and amplifies the signals from receptor tyrosine kinases. A recent study demonstrated that Gab2 was over-expressed in breast cancers and metastatic melanomas, and Gab2 was an oncogenic protein. However, the roles of Gab2 in lung cancers are largely unknown.
  
  
  METHODS
  In this study, to investigate whether Gab2 expression could be a characteristic of lung cancers, we analyzed the expression of Gab2 in 88 lung frozen tissue samples and 122 paraffin-embedded tissue specimens, using quantitative real-time-PCR, immunohistochemistry and western blot.
  
  
  RESULTS
  We found that the positive expression rate of Gab2 in the tumor tissues, as detected by immunohistochemistry, 62.5% in squamous cell cancers, 51.35% in adenocarcinomas, and 75% in other types of lung cancers, was significantly higher than that (12%) in normal lung tissues. The mRNA expression detected by quantitative real-time-PCR and protein expression detected by western blotting in different groups were consistent with the immunohistochemical results.
  
  
  CONCLUSIONS
  Our data indicate that Gab2 is over-expressed in malignant lung tissues compared with that in normal lung tissues, and suggest that Gab2 expression may play a role in lung cancer development.
authors.affiliation: Department of Pathology, Tianjin Medical University, Heping District, Tianjin 300070, China.
authors.forename: Xiu-Li
authors.initials: XL
authors.lastname: Xu
authors.forename: Xia
authors.initials: X
authors.lastname: Wang
authors.forename: Zhao-Li
authors.initials: ZL
authors.lastname: Chen
authors.forename: Min
authors.initials: M
authors.lastname: Jin
authors.forename: Wu
authors.initials: W
authors.lastname: Yang
authors.forename: Guo-Fan
authors.initials: GF
authors.lastname: Zhao
authors.forename: Jun-Wen
authors.initials: JW
authors.lastname: Li
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D000230:Adenocarcinoma / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism*; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D014408:Biomarkers, Tumor; C490895:GAB2 protein, human; D012333:RNA, Messenger