title: A novel amperometric hydrogen peroxide biosensor based on electrospun Hb-collagen composite.
abstract: In this paper, the hemoglobin (Hb)-collagen microbelt modified electrode with three-dimensional configuration was fabricated via the electrospinning method. Direct electron transfer of the Hb immobilized into the electrospun collagen microbelts was greatly facilitated. The apparent heterogeneous electron transfer rate constant (k(s)) was calculated to be 270.6s⁻¹. The electrospun Hb-collagen microbelt modified electrode showed an excellent bioelectrocatalytic activity toward the reduction of H₂O₂. The amperometric response of the biosensor varied linearly with the H₂O₂ concentration ranging from 5 × 10⁻⁶molL⁻¹ to 30×10⁻⁶molL⁻¹, with a detection limit of 0.37 × 10⁻⁶molL⁻¹ (signal-to-noise ratio of 3). The apparent Michaelis-Menten constant (K(m)(app)) was 77.7 μmolL⁻¹. The established biosensor exhibited fast amperometric response, high sensitivity, good reproducibility and stability.
authors.affiliation: Center for Biomedical Materials and Engineering, Harbin Engineering University, No. 145, Na-Tong-Da Street, Nan-Gang District, Harbin 150001, China.
authors.forename: F
authors.initials: F
authors.lastname: Guo
authors.forename: X X
authors.initials: XX
authors.lastname: Xu
authors.forename: Z Z
authors.initials: ZZ
authors.lastname: Sun
authors.forename: J X
authors.initials: JX
authors.lastname: Zhang
authors.forename: Z X
authors.initials: ZX
authors.lastname: Meng
authors.forename: W
authors.initials: W
authors.lastname: Zheng
authors.forename: H M
authors.initials: HM
authors.lastname: Zhou
authors.forename: B L
authors.initials: BL
authors.lastname: Wang
authors.forename: Y F
authors.initials: YF
authors.lastname: Zheng
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D003094:Collagen / Q000737:chemistry*; D004563:Electrochemistry / Q000379:methods; D006454:Hemoglobins / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry*
chemical_list: D006454:Hemoglobins; D003094:Collagen; D006861:Hydrogen Peroxide