title: Enzymatic assay of phosphatidylethanolamine in serum using amine oxidase from Arthrobacter sp.
abstract: BACKGROUND
  In human serum, as for phospholipids not containing choline, phosphatidylethanolamine (PE) exists approximately 5% in a whole phospholipid. PE is well known as one of the main components of biological membranes, and also plays important roles that contribute to apoptosis and cell signaling. However, it could not measure PE with other phospholipids due to a lack of choline in them.
  
  
  METHODS
  Using an amine oxidase (EC 1.4.3.6), from Arthrobacter species, a simple and rapid enzymatic assay for measurements of PE in serum was established. That assay used the Hitachi 7170 analyzer to evaluate the analytical performance.
  
  
  RESULTS
  The average within-run CVs were 0.38-1.27% (n=20) at 69-160 μmol/l. The correlation between values obtained with the present method (y) and the high-performance liquid chromatography (HPLC) method (x) was: y=0.944x+9.441 (r=0.977, S(y|x)=5.82, n=34). In addition, the reference interval of healthy subjects was 115±45 μmol/l.
  
  
  CONCLUSIONS
  This new enzymatic method shows a high specificity for serum PE and can be easily applied to an automated analyzer. The present method is available as a novel marker of changes in the clinical condition of serum phospholipids.
authors.affiliation: Division of Biological Science and Technology Department of Health Sciences, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka City, Japan. hokazono@shs.kyushu-u.ac.jp
authors.forename: Eisaku
authors.initials: E
authors.lastname: Hokazono
authors.forename: Hideto
authors.initials: H
authors.lastname: Tamezane
authors.forename: Taeko
authors.initials: T
authors.lastname: Hotta
authors.forename: Yuzo
authors.initials: Y
authors.lastname: Kayamori
authors.forename: Susumu
authors.initials: S
authors.lastname: Osawa
mesh_terms: D006631:Amine Oxidase (Copper-Containing) / Q000737:chemistry* / Q000378:metabolism; D001173:Arthrobacter / Q000201:enzymology*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D010714:Phosphatidylethanolamines / Q000097:blood* / Q000378:metabolism; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D010714:Phosphatidylethanolamines; D006631:Amine Oxidase (Copper-Containing)