title: The ganglioside profile of human bladder cell lines and the effect of exogenous gangliosides on their growth.
abstract: The ganglioside composition of three human bladder cell lines, two tumourigenic (RT112 and EJ138) and one non-tumourigenic (Hu609) were analysed by thin-layer chromatography and mass spectroscopy. Sub-confluent cultures of the three cell lines expressed mainly monosialogangliosides. Xenografts of EJ138 cells expressed nearly twice as much GM(3) as tissue cultured cells. Proliferation of Hu609 and EJ138 cells measured by H-3-thymidine incorporation was increased by GM(3) and further enhanced by addition of EGF. Paradoxically the migration of all three cell lines was inhibited by a mixture of monosialogangliosides and the inhibition was abolished by EGF.
authors.affiliation: CHRISTIE HOSP & HOLT RADIUM INST,MANCHESTER M20 9BX,LANCS,ENGLAND.
authors.forename: M
authors.initials: M
authors.lastname: Slevin
authors.forename: S
authors.initials: S
authors.lastname: Kumar
authors.forename: J
authors.initials: J
authors.lastname: Gaffney