title: Quality control guidelines for amphotericin B, Itraconazole, posaconazole, and voriconazole disk diffusion susceptibility tests with nonsupplemented Mueller-Hinton Agar (CLSI M51-A document) for nondermatophyte Filamentous Fungi.
abstract: Although Clinical and Laboratory Standards Institute (CLSI) disk diffusion assay standard conditions are available for susceptibility testing of filamentous fungi (molds) to antifungal agents, quality control (QC) disk diffusion zone diameter ranges have not been established. This multicenter study documented the reproducibility of tests for one isolate each of five molds (Paecilomyces variotii ATCC MYA-3630, Aspergillus fumigatus ATCC MYA-3626, A. flavus ATCC MYA-3631, A. terreus ATCC MYA-3633, and Fusarium verticillioides [moniliforme] ATCC MYA-3629) and Candida krusei ATCC 6258 by the CLSI disk diffusion method (M51-A document). The zone diameter ranges for selected QC isolates were as follows: P. variotii ATCC MYA-3630, amphotericin B (15 to 24 mm), itraconazole (20 to 31 mm), and posaconazole (33 to 43 mm); A. fumigatus ATCC MYA-3626, amphotericin B (18 to 25 mm), itraconazole (11 to 21 mm), posaconazole (28 to 35 mm), and voriconazole (25 to 33 mm); and C. krusei, amphotericin B (18 to 27 mm), itraconazole (18 to 26 mm), posaconazole (28 to 38 mm), and voriconazole (29 to 39 mm). Due to low testing reproducibility, zone diameter ranges were not proposed for the other three molds.
authors.affiliation: VCU Medical Center, Richmond, Virginia 23221, USA. avingrof@verizon.net
authors.forename: A
authors.initials: A
authors.lastname: Espinel-Ingroff
authors.forename: E
authors.initials: E
authors.lastname: Canton
authors.forename: A
authors.initials: A
authors.lastname: Fothergill
authors.forename: M
authors.initials: M
authors.lastname: Ghannoum
authors.forename: E
authors.initials: E
authors.lastname: Johnson
authors.forename: R N
authors.initials: RN
authors.lastname: Jones
authors.forename: L
authors.initials: L
authors.lastname: Ostrosky-Zeichner
authors.forename: W
authors.initials: W
authors.lastname: Schell
authors.forename: D L
authors.initials: DL
authors.lastname: Gibbs
authors.forename: A
authors.initials: A
authors.lastname: Wang
authors.forename: J
authors.initials: J
authors.lastname: Turnidge
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D003470:Culture Media / Q000737:chemistry*; D005658:Fungi / Q000187:drug effects*; D008826:Microbial Sensitivity Tests / Q000379:methods / Q000592:standards; D009172:Mycology / Q000379:methods* / Q000592:standards*; D011786:Quality Control; D015203:Reproducibility of Results
chemical_list: D000935:Antifungal Agents; D003470:Culture Media