title: The effect of injection technique on the passive spread of astrocytes following transplantation into rat spinal cord white matter.
abstract: Bisbenzimide-labelled astrocytes were transplanted into the spinal white matter of the rat using three different injection techniques and the variability of the longitudinal distance over which they were found was compared 30 min later. Cells spread up to 5.02 mm and the greatest variability was seen when they were injected as a bolus (54%), compared with 26% when injected over 2 min. These results show the importance of establishing the extent of passive spread of cells and its variability when performing studies in cell migration.
authors.affiliation: Department of Clinical Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge CB3 OES, UK.
authors.forename: N J
authors.initials: NJ
authors.lastname: Olby
authors.forename: M T
authors.initials: MT
authors.lastname: O'leary
authors.forename: M P
authors.initials: MP
authors.lastname: Targett
authors.forename: W F
authors.initials: WF
authors.lastname: Blakemore