title: Radiation-initiated, immortal Syrian hamster embryo fibroblasts release elevated levels of H2O2 and show divergent MnSOD and catalase activities.
abstract: To characterize molecular events associated with the neoplastic conversion of primary cells by ionizing radiation, we studied the activities and mRNA expression of manganese superoxide dismutase (MnSOD) and catalase (CAT) in Syrian hamster embryo fibroblasts during the early stages of immortalization after treatment with gamma-rays. The irradiated cells showed divergent MnSOD and CAT responses relative to unirradiated controls. At passage 6, MnSOD activity was increased about 50-fold, although the concentration of MnSOD mRNA increased only 1.6-fold. By contrast, CAT activity diminished 2-fold despite an increase of 1.6-fold in the concentration of CAT mRNA. This divergence between the MnSOD and CAT activities was maintained upon culturing and, at passage 12, MnSOD was 35-fold increased and CAT 3.7-fold decreased, relative to unirradiated cells. The amount of H2O2 released into the culture medium by the radiation-initiated cells was 6-fold greater than in control media. Because H2O2 is a causative agent in the induction of malignant transformation in vitro, our results suggest that the elevated production of H2O2 caused by the imbalance between the activities of MnSOD and CAT may participate in the immortalization and subsequent malignant conversion of Syrian hamster embryo fibroblasts by ionizing radiation.
authors.affiliation: GEORGETOWN UNIV,MED CTR,DEPT RADIAT MED,WASHINGTON,DC 20007. GEORGETOWN UNIV,MED CTR,LUNG BIOL LABS,WASHINGTON,DC 20007.
authors.forename: G
authors.initials: G
authors.lastname: Otero
authors.forename: A
authors.initials: A
authors.lastname: Berkovich
authors.forename: L
authors.initials: L
authors.lastname: Clerch
authors.forename: D
authors.initials: D
authors.lastname: Massaro
authors.forename: V
authors.initials: V
authors.lastname: Notario