title: p53 protein accumulation and p53 gene alterations (RFLP, VNTR and p53 gene mutations) in non-invasive versus invasive human transitional bladder cancer.
abstract: Eleven non-invasive and 24 invasive transitional cell bladder cancers were analysed for molecular alterations to the p53 gene and nuclear accumulation of the p53 protein. 9% (1/11) of non-invasive rumours and 21% (5/24) of invasive tumours revealed nuclear accumulation in more than 50% of the tumour cells. PCR analysis of D17S30 showed loss of heterozygosity (LOH) in invasive tumours (3/24; 12%). Two invasive tumours harboured point mutations in exon 6 and exon 7, respectively (8%). Our results indicate that p53 protein overexpression correlates with tumour progression, p53 gene mutations and LOH detected by PCR.
authors.affiliation: UNIV BASEL HOSP,RES CTR,ZLF,LAB 405,CH-4031 BASEL,SWITZERLAND. INST PATHOL,CH-4003 BASEL,SWITZERLAND. ST CLARA HOSP,SECT ONCOL,DEPT MED,CH-4016 BASEL,SWITZERLAND.
authors.forename: R
authors.initials: R
authors.lastname: Shipman
authors.forename: P
authors.initials: P
authors.lastname: Schraml
authors.forename: H
authors.initials: H
authors.lastname: Moch
authors.forename: M
authors.initials: M
authors.lastname: Colombi
authors.forename: G
authors.initials: G
authors.lastname: Sauter
authors.forename: M
authors.initials: M
authors.lastname: Mihatsch
authors.forename: C
authors.initials: C
authors.lastname: Ludwig