title: Morphometric analysis of human sciatic nerve perineurial collagen type IV content.
abstract: OBJECTIVE
  Aging is the process which unavoidably alters structure and function of the basal membranes in humans. Though, collagen type IV presents the most prominent component of the basal membranes, we estimated its presence in the perineurium of the human sciatic nerve samples during the aging process.
  
  
  METHODS
  Material was 12 sciatic nerve samples, obtained from cadavers whose age ranged from 36 to 84 years. Cadavers were classified into three age groups: first which age ranged from 35 to 54 years, second which age ranged from 55 to 74 years and third which included cases older than 75 years. Tissue slices were further stained by labeled streptavidin-biotin method with collagen type IV monoclonal antibody and analyzed with light microscope under 100× lens magnification with oil immersion. Digital images of sciatic nerve perineurium were further processed and analyzed with ImageJ software.
  
  
  RESULTS
  Our results showed that there is statistically significant increase of perineurial area, perimeter, collagen type IV area, and collagen type IV area per perineurial perimeter unit in the third age group. These parameters also increased in the second age group, but this increase was not significant. Multiple regression analysis showed that beside fascicular size, age more significantly predict perineurial collagen type IV content.
  
  
  CONCLUSIONS
  Results of morphometric and statistical analysis pointed to the conclusion that there is significant increase of sciatic nerve perineurial thickness during the aging process. This increase might represent the consequence of perineurial collagen type IV deposition with aging.
authors.affiliation: Department of Anatomy, Faculty of Medicine, Nis, Serbia. sdugren@sbb.rs
authors.forename: Sladjana
authors.initials: S
authors.lastname: Ugrenović
authors.forename: Ivan
authors.initials: I
authors.lastname: Jovanović
authors.forename: Ljiljana
authors.initials: L
authors.lastname: Vasović
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging; D001485:Basement Membrane / Q000737:chemistry* / Q000648:ultrastructure*; D024141:Collagen Type IV / Q000032:analysis* / Q000648:ultrastructure*; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008297:Male; D008853:Microscopy; D008875:Middle Aged; D012584:Sciatic Nerve / Q000737:chemistry* / Q000648:ultrastructure*
chemical_list: D024141:Collagen Type IV