title: Manipulation of the gut microbiota in C57BL/6 mice changes glucose tolerance without affecting weight development and gut mucosal immunity.
abstract: Inflammatory diseases such as type 2 diabetes (T2D) in humans and mice are under the influence of the composition of the gut microbiota (GM). It was previously demonstrated that treating Lep(ob) mice with antibiotics improved glucose tolerance. However, wild type C57BL/6J mice may also exhibit plasma glucose intolerance reminiscent of human T2D. We hypothesized that antibiotic treatment in C57BL/6 mice would have an impact on glucose tolerance without affecting weight and gut immunology. When compared to mice treated with erythromycin or the controls, treatment for five weeks with ampicillin improved glucose tolerance without significantly affecting the weight or the number of gut mucosal regulatory T cells, tolerogenic dendritic cells or T helper cells type 1. 16S rRNA gene based denaturing gradient gel electrophoresis profiles clearly clustered according to treatment and showed that antibiotic treatment reduced GM diversity. It is concluded that antibiotic treatment changes glucose metabolism as well as the composition of the GM in C57BL/6 mice, and that this does not seem to be correlated to weight development in the mice.
authors.affiliation: Department of Veterinary Disease Biology, Faculty of Life Sciences, University of Copenhagen, Grønnegårdsvej 18, DK-1870 Frederiksberg, Denmark. gvbn@vet.dtu.dk
authors.forename: Gunilla Veslemøy
authors.initials: GV
authors.lastname: Bech-Nielsen
authors.forename: Camilla Hartmann Friis
authors.initials: CH
authors.lastname: Hansen
authors.forename: Majbritt Ravn
authors.initials: MR
authors.lastname: Hufeldt
authors.forename: Dennis Sandris
authors.initials: DS
authors.lastname: Nielsen
authors.forename: Bent
authors.initials: B
authors.lastname: Aasted
authors.forename: Finn Kvist
authors.initials: FK
authors.lastname: Vogensen
authors.forename: Tore
authors.initials: T
authors.lastname: Midtvedt
authors.forename: Axel Kornerup
authors.initials: AK
authors.lastname: Hansen
mesh_terms: D000667:Ampicillin / Q000494:pharmacology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D001786:Blood Glucose; D001835:Body Weight / Q000502:physiology*; D004917:Erythromycin / Q000494:pharmacology; D005260:Female; D041981:Gastrointestinal Tract / Q000276:immunology / Q000382:microbiology*; D018149:Glucose Intolerance / Q000382:microbiology*; D005951:Glucose Tolerance Test; D007413:Intestinal Mucosa / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL
chemical_list: D000900:Anti-Bacterial Agents; D001786:Blood Glucose; D004917:Erythromycin; D000667:Ampicillin