title: Immunomodulatory effects of cinobufagin isolated from Chan Su on activation and cytokines secretion of immunocyte in vitro.
abstract: The objective of this study was to evaluate the immunomodulatory effects of cinobufagin (CBG) isolated from Chan Su (Venenum Bufonis) in vitro. In this paper, our results show that CBG significantly stimulated cell proliferation of splenocytes and peritoneal macrophages (PMΦ) and markedly enhanced the phagocytic activation of PMΦ. CBG also significantly increased CD4(+)CD8(+) double-positive T-cell populations and the percentage of S-phase cells of splenic lymphocytes. The levels of several Th1 cytokines, including interferon-γ and tumor necrosis factor-α, are significantly increased after CBG treatment, whereas the levels of the Th2 cytokine interleukin-4 and interleukin-10 are significantly decreased. As a result, the ratio of Th1/Th2 also increased. Taken together, these results indicated that CBG had potential immune system regulatory effects and suggested that this compound could be developed as a novel immunotherapeutic agent to treat immune-mediated diseases such as cancer.
authors.affiliation: College of Animal Science and Veterinary Medicine, Jilin University, Changchun, China.
authors.forename: Xiao-Liang
authors.initials: XL
authors.lastname: Wang
authors.forename: Guang-Hou
authors.initials: GH
authors.lastname: Zhao
authors.forename: Jin
authors.initials: J
authors.lastname: Zhang
authors.forename: Qi-Yun
authors.initials: QY
authors.lastname: Shi
authors.forename: Wei-Xiao
authors.initials: WX
authors.lastname: Guo
authors.forename: Xiu-Li
authors.initials: XL
authors.lastname: Tian
authors.forename: Jia-Zhang
authors.initials: JZ
authors.lastname: Qiu
authors.forename: Li-Zi
authors.initials: LZ
authors.lastname: Yin
authors.forename: Xu-Ming
authors.initials: XM
authors.lastname: Deng
authors.forename: Yu
authors.initials: Y
authors.lastname: Song
mesh_terms: D000664:Amphibian Venoms / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D002018:Bufanolides / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology*; D016207:Cytokines / Q000187:drug effects* / Q000378:metabolism; D007155:Immunologic Factors / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology*; D007371:Interferon-gamma / Q000032:analysis; D016753:Interleukin-10 / Q000032:analysis; D015847:Interleukin-4 / Q000032:analysis; D017737:Macrophages, Peritoneal / Q000187:drug effects*; D015394:Molecular Structure; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D018417:Th1 Cells / Q000187:drug effects; D018418:Th2 Cells / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
chemical_list: D000664:Amphibian Venoms; D002018:Bufanolides; D016207:Cytokines; D007155:Immunologic Factors; D014409:Tumor Necrosis Factor-alpha; C095591:chan su; D016753:Interleukin-10; D015847:Interleukin-4; D007371:Interferon-gamma; C002471:cinobufagin