title: Development of an inactivated candidate vaccine against Chandipura virus (Rhabdoviridae: Vesiculovirus).
abstract: A Vero cell based vaccine candidate against Chandipura (CHP) virus (Rhabdoviridae: Vesiculovirus), was developed and evaluated for immunogenicity in mice. Virus was purified by ultracentrifugation on 30% glycerol cushion followed by differential centrifugation on 10-60% sucrose gradient and inactivated with β-propio lactone at a concentration of 1:3500. The inactivated product was blended with aluminium phosphate (3%) and immunized 4-week-old Swiss albino mice. Neutralizing antibodies in the range of 1:10 to 160 and 1:80 to 1:320 was detected with 85% and 100% sero-conversion after 2nd and 3rd dose, respectively. All the immunized mice with antibody titer above 1:20 survived live virus challenge. The vaccine candidate has potential to be an efficient vaccine against CHP virus.
authors.affiliation: National Institute of Virology, Indian Council of Medical Research, Sus Road, Pashan, Pune 411021, India.
authors.forename: R S
authors.initials: RS
authors.lastname: Jadi
authors.forename: A B
authors.initials: AB
authors.lastname: Sudeep
authors.forename: P V
authors.initials: PV
authors.lastname: Barde
authors.forename: V A
authors.initials: VA
authors.lastname: Arankalle
authors.forename: A C
authors.initials: AC
authors.lastname: Mishra
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D002522:Chlorocebus aethiops; D007114:Immunization; D051379:Mice; D018353:Rhabdoviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000821:virology; D016896:Treatment Outcome; D015164:Vaccines, Inactivated / Q000008:administration & dosage / Q000276:immunology*; D014709:Vero Cells; D018116:Vesiculovirus / Q000276:immunology* / Q000472:pathogenicity / Q000502:physiology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D015164:Vaccines, Inactivated; D014765:Viral Vaccines