title: Spectrum-to-spectrum searching using a proteome-wide spectral library.
abstract: The unambiguous assignment of tandem mass spectra (MS/MS) to peptide sequences remains a key unsolved problem in proteomics. Spectral library search strategies have emerged as a promising alternative for peptide identification, in which MS/MS spectra are directly compared against a reference library of confidently assigned spectra. Two problems relate to library size. First, reference spectral libraries are limited to rediscovery of previously identified peptides and are not applicable to new peptides, because of their incomplete coverage of the human proteome. Second, problems arise when searching a spectral library the size of the entire human proteome. We observed that traditional dot product scoring methods do not scale well with spectral library size, showing reduction in sensitivity when library size is increased. We show that this problem can be addressed by optimizing scoring metrics for spectrum-to-spectrum searches with large spectral libraries. MS/MS spectra for the 1.3 million predicted tryptic peptides in the human proteome are simulated using a kinetic fragmentation model (MassAnalyzer version2.1) to create a proteome-wide simulated spectral library. Searches of the simulated library increase MS/MS assignments by 24% compared with Mascot, when using probabilistic and rank based scoring methods. The proteome-wide coverage of the simulated library leads to 11% increase in unique peptide assignments, compared with parallel searches of a reference spectral library. Further improvement is attained when reference spectra and simulated spectra are combined into a hybrid spectral library, yielding 52% increased MS/MS assignments compared with Mascot searches. Our study demonstrates the advantages of using probabilistic and rank based scores to improve performance of spectrum-to-spectrum search strategies.
authors.affiliation: Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO 80309, USA.
authors.forename: Chia-Yu
authors.initials: CY
authors.lastname: Yen
authors.forename: Stephane
authors.initials: S
authors.lastname: Houel
authors.forename: Natalie G
authors.initials: NG
authors.lastname: Ahn
authors.forename: William M
authors.initials: WM
authors.lastname: Old
mesh_terms: D000465:Algorithms; D003627:Data Interpretation, Statistical; D006801:Humans; D010446:Peptide Fragments / Q000032:analysis*; D019151:Peptide Library; D010449:Peptide Mapping / Q000379:methods*; D011336:Probability; D020543:Proteome / Q000032:analysis*; D012372:ROC Curve; D012016:Reference Values; D053719:Tandem Mass Spectrometry
chemical_list: D010446:Peptide Fragments; D019151:Peptide Library; D020543:Proteome