title: Degradation of cyhalofop-butyl (CyB) by Pseudomonas azotoformans strain QDZ-1 and cloning of a novel gene encoding CyB-hydrolyzing esterase.
abstract: Cyhalofop-butyl (CyB) is a widely used aryloxyphenoxy propanoate (AOPP) herbicide for control of grasses in rice fields. Five CyB-degrading strains were isolated from rice field soil and identified as Agromyces sp., Stenotrophomonas sp., Aquamicrobium sp., Microbacterium sp., and Pseudomonas azotoformans; the results revealed high biodiversity of CyB-degrading bacteria in rice soil. One strain, P. azotoformans QDZ-1, degraded 84.5% of 100 mg L(-1) CyB in 5 days of incubation in a flask and utilized CyB as carbon source for growth. Strain QDZ-1 could also degrade a wide range of other AOPP herbicides. An esterase gene, chbH, which hydrolyzes CyB to cyhalofop acid (CyA), was cloned from strain QDZ-1 and functionally expressed. A chbH-disrupted mutant dchbH was constructed by insertion mutation. Mutant dchbH could not degrade and utilize CyB, suggesting that chbH was the only esterase gene responsible for CyB degradation in strain QDZ-1. ChbH hydrolyzed all AOPP herbicides tested as well as permethrin. The catalytic efficiency of ChbH toward different AOPP herbicides followed the order quizalofop-P-ethyl ≈ fenoxaprop-P-ethyl > CyB ≈ fluazifop-P-butyl > diclofop-methyl ≈ haloxyfop-P-methyl; the results indicated that the chain length of the alcohol moiety strongly affected the biodegradability of the AOPP herbicides, whereas the substitutions in the aromatic ring had only a slight influence.
authors.affiliation: Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu 210095, People's Republic of China.
authors.forename: Zhi-Juan
authors.initials: ZJ
authors.lastname: Nie
authors.forename: Bao-Jian
authors.initials: BJ
authors.lastname: Hang
authors.forename: Shu
authors.initials: S
authors.lastname: Cai
authors.forename: Xiang-Ting
authors.initials: XT
authors.lastname: Xie
authors.forename: Jian
authors.initials: J
authors.lastname: He
authors.forename: Shun-Peng
authors.initials: SP
authors.lastname: Li
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001673:Biodegradation, Environmental; D002073:Butanes / Q000378:metabolism*; D003001:Cloning, Molecular; D004950:Esterases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D006540:Herbicides / Q000378:metabolism*; D007700:Kinetics; D008969:Molecular Sequence Data; D009570:Nitriles / Q000378:metabolism*; D010802:Phylogeny; D011549:Pseudomonas / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012988:Soil Microbiology
chemical_list: D001426:Bacterial Proteins; D002073:Butanes; D006540:Herbicides; D009570:Nitriles; C501583:cyhalofop-butyl; D004950:Esterases