title: Amelogenin promotes odontoblast-like MDPC-23 cell differentiation via activation of ERK1/2 and p38 MAPK.
abstract: Amelogenin (AMG) is a highly conserved protein secreted by ameloblasts. Some research indicates that AMG might induce the differentiation and maturation of odontoblasts. The aim of this study was to clarify the function of AMG during the differentiation of odontoblast-like MDPC-23 cells. The results revealed that the alkaline phosphatase activity and the number of mineralized nodules were significantly enhanced in AMG-overexpressing MDPC-23 cells during the mineralization process. Tissue-specific markers such as dentin matrix protein 1 and dentin sialophosphoprotein also elevated significantly, indicating the cell differentiation and maturation process. Furthermore, AMG could upregulate the phosphorylation levels of ERK1/2 and p38 MAPK. However, JNK, another MAPK pathway molecule, didn't change the activity at all. And the differentiation induced by AMG was abrogated when the MDPC-23 cells were treated with U0126 and SB203580, the inhibitors of ERK1/2 and p38, respectively. Taken together, our present results showed that AMG could promote the differentiation of odontoblast-like MDPC-23 cells via ERK1/2 and p38 MAPK pathways.
authors.affiliation: Department of Stomatology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, People's Republic of China.
authors.forename: Naihui
authors.initials: N
authors.lastname: Yao
authors.forename: Shiting
authors.initials: S
authors.lastname: Li
authors.forename: Yong
authors.initials: Y
authors.lastname: Jiang
authors.forename: Songbo
authors.initials: S
authors.lastname: Qiu
authors.forename: Yinghui
authors.initials: Y
authors.lastname: Tan
mesh_terms: D000256:Adenoviridae; D000469:Alkaline Phosphatase / Q000378:metabolism; D053523:Amelogenin / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D000943:Antigens, Differentiation / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation; D002460:Cell Line; D003001:Cloning, Molecular; D057075:Enzyme Assays; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D005822:Genetic Vectors; D051379:Mice; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism*; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism*; D009804:Odontoblasts / Q000166:cytology*; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D010766:Phosphorylation; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*; D012795:Sialoglycoproteins / Q000235:genetics / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*; D000099311:Dentin Sialophosphoprotein
chemical_list: D000469:Alkaline Phosphatase; D053523:Amelogenin; D000943:Antigens, Differentiation; D016326:Extracellular Matrix Proteins; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D010750:Phosphoproteins; D011994:Recombinant Proteins; D012795:Sialoglycoproteins; D048051:p38 Mitogen-Activated Protein Kinases; D000099311:Dentin Sialophosphoprotein; C513308:Dmp1 protein, mouse