title: Antifungal susceptibilities of Cryptococcus neoformans cerebrospinal fluid isolates from AIDS patients in Kenya.
abstract: Poor susceptibility of Cryptococcus neoformans to fluconazole (FLC) is a matter of concern among clinicians in Africa. The emergence of resistance to FLC was recently reported in Kenya, but it is not known whether it is widespread. Thus, there is need for more antifungal drug susceptibility studies in Kenya. The aim of this study was to measure the in vitro antifungal drug susceptibilities of incident C. neoformans isolates from acquired immunodeficiency syndrome patients in Kenya. Antifungal susceptibility testing was performed in 67 C. neoformans isolates by broth microdilution method as outlined in the Clinical and Laboratory Standards Institute document M27-A3 using FLC, amphotericin B (AMB), voriconazole (VOR), ravuconazole (RAV) and flucytosine (5-FC). Isolates were grown on l-canavanine glycine bromothymol blue medium for serotype identification. Six per cent of the isolates were identified as C. neoformans var. gattii serotype B or C and 94% as C. neoformans var. neoformans. All isolates tested were susceptible to AMB, VOR and RAV (100%), and high susceptibilities were seen to FLC (97%), and 5-FC (90%). Only 3% and 10% of the isolates' susceptibility to FLC and 5-FC, respectively, was dose-dependent or intermediate. These results demonstrate high susceptibilities of incident C. neoformans isolates to FLC and AMB, antifungals used for treatment of cryptococcal meningitis in Kenya.
authors.affiliation: School of Public Health, University of Alabama at Birmingham, Birmingham, AL 35294-0022, USA.
authors.forename: Rennatus
authors.initials: R
authors.lastname: Mdodo
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Moser
authors.forename: Walter
authors.initials: W
authors.lastname: Jaoko
authors.forename: John
authors.initials: J
authors.lastname: Baddley
authors.forename: Peter
authors.initials: P
authors.lastname: Pappas
authors.forename: Mirjam-Colette
authors.initials: MC
authors.lastname: Kempf
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Aban
authors.forename: Susan
authors.initials: S
authors.lastname: Odera
authors.forename: Pauline
authors.initials: P
authors.lastname: Jolly
mesh_terms: D000328:Adult; D000935:Antifungal Agents / Q000494:pharmacology*; D002555:Cerebrospinal Fluid / Q000382:microbiology*; D003453:Cryptococcosis / Q000382:microbiology*; D003455:Cryptococcus neoformans / Q000187:drug effects* / Q000302:isolation & purification*; D003470:Culture Media / Q000737:chemistry; D005260:Female; D015658:HIV Infections / Q000150:complications; D006801:Humans; D007630:Kenya; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged
chemical_list: D000935:Antifungal Agents; D003470:Culture Media