title: Secondary metabolites from Glycine soja and their growth inhibitory effect against Spodoptera litura.
abstract: The wild soybean (Glycine soja Sieb. et Zucc) has been reported to be relatively resistant to insect and pathogenic pests. However, the responsible secondary metabolites in the aerial part of this important plant are largely unknown. From the aerial part of G. soja, 13 compounds were isolated and identified, including seven isoflavonoids (1-7), a cyclitol (8), two sterol derivatives (9 and 10), and three triterpenoids (11-13). Compound 7 is a new isoflavonoid, and compounds 9 and 10 are reported as natural products for the first time. The growth inhibitory activity of 1, 3, 4, and 8 against the larvae of Spodoptera litura was investigated. The most abundant isoflavonoid in the aerial part of G. soja, daidzein (1), which could not be metabolized by S. litura, was found to inhibit the insect larvae growth significantly in 3 days after feeding diets containing the compound. Compounds 3, 4, and 8, which could be partially or completely metabolized, were inactive. Our results suggested that the isoflavonoid daidzein (1) might function as a constitutive defense component in G. soja against insect pests.
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, Yunnan, People's Republic of China.
authors.forename: Yan-Ying
authors.initials: YY
authors.lastname: Zhou
authors.forename: Shi-Hong
authors.initials: SH
authors.lastname: Luo
authors.forename: Ting-Shuang
authors.initials: TS
authors.lastname: Yi
authors.forename: Chun-Huan
authors.initials: CH
authors.lastname: Li
authors.forename: Qian
authors.initials: Q
authors.lastname: Luo
authors.forename: Juan
authors.initials: J
authors.lastname: Hua
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Sheng-Hong
authors.initials: SH
authors.lastname: Li
mesh_terms: D000818:Animals; D007529:Isoflavones / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D007814:Larva / Q000187:drug effects / Q000254:growth & development; D010936:Plant Extracts / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D013025:Glycine max / Q000737:chemistry / Q000378:metabolism*; D018411:Spodoptera / Q000187:drug effects* / Q000254:growth & development*
chemical_list: D007529:Isoflavones; D010936:Plant Extracts