title: CD44 with variant exons 8-10 in colorectal tumors.
abstract: Using a fusion protein encoded by variant exon (v) 8-10 of CD44, a monoclonal antibody (mAb) 44-1V reactive with v9 product was generated. Western immunoblots and reverse transcriptase-polymerase chain reaction/Southern blot revealed that a 130 kilodalton component containing v8-10 products was specifically expressed in colorectal and gastric cancer cell lines. Immunohistological examination of 179 pairs of colorectal cancer and normal tissues revealed that about 47% of cancers expressed the CD44 variant (CD44v8-10), and its expression correlated with hematogenous and lymph node metastasis. These findings suggest the usefulness of CD44v8-10 in the prediction of colorectal cancer metastasis.
authors.affiliation: NAGASAKI UNIV,SCH MED,DEPT ONCOL,NAGASAKI 852,JAPAN. FUKUI MED SCH,DEPT SURG 1,FUKUI 91011,JAPAN. MIE UNIV,SCH MED,DEPT INTERNAL MED 2,TSU,MIE 514,JAPAN.
authors.forename: T
authors.initials: T
authors.lastname: Goi
authors.forename: A
authors.initials: A
authors.lastname: Yamaguchi
authors.forename: K
authors.initials: K
authors.lastname: Takeuchi
authors.forename: G
authors.initials: G
authors.lastname: Nakagawa
authors.forename: S
authors.initials: S
authors.lastname: Yamashiro
authors.forename: K
authors.initials: K
authors.lastname: Furukawa
authors.forename: T
authors.initials: T
authors.lastname: Urano
authors.forename: H
authors.initials: H
authors.lastname: Shiku