title: Evaluation of prognostic-significance of p53 gene alterations in patients with surgically resected lung-cancer.
abstract: Clinical significance of p53 gene alterations, as a prognostic factor, was assessed in 69 patients with surgically resected lung cancer. The p53 gene alterations (exon 5-9) were examined by the polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) method of genomic DNA. The p53 gene alterations were detected in all histological types of lung cancer, with a positive rate of 45% (31/69). In the alteration-positive group, patients in the advanced stages of III and IV were seen more frequently than in the negative group (58% vs. 21%, p<0.05). Such a difference was not observed in other parameters such as age, gender, histological type and smoking habit. The prognosis was, on a whole, poorer in the alteration-positive group than for the -negative one (5-year survival rate: 19.3% vs. 40.6%, MST: 17 months vs. 36 months), but the difference did not reach statistical significance. However, in the case of females (p<0.05), adenocarcinoma (p<0.01), early stages of I and II (p<0.05) and non-smokers (p<0.005), a significantly poorer prognosis was observed in the gene alteration-positive group than for the -negative one. These results suggest that the p53 gene alteration may be a useful prognostic factor in certain subgroups with lung resected for cancer.
authors.affiliation: TOYAMA MED & PHARMACEUT UNIV,DEPT INTERNAL MED 1,TOYAMA 93001,JAPAN. TOYAMA MED & PHARMACEUT UNIV,DEPT PATHOL 1,TOYAMA 93001,JAPAN. TOYAMA MED & PHARMACEUT UNIV,DEPT SURG 1,TOYAMA 93001,JAPAN. TOYAMA PREFECTURAL CENT HOSP,DEPT CHEST & CARDIOVASC SURG,TOYAMA 930,JAPAN. ISHIKAWA PREFECTURAL CENT HOSP,DEPT THORAC SURG,KANAZAWA,ISHIKAWA 920,JAPAN. KOSEIREN TAKAOKA HOSP,DEPT SURG,TAKAOKA,TOYAMA 933,JAPAN. TOYAMA CITY HOSP,DEPT CHEST SURG,TOYAMA 939,JAPAN.
authors.forename: T
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authors.lastname: Kashii
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authors.lastname: Mizushima
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authors.lastname: Lima
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authors.lastname: Noto
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authors.lastname: Sato
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authors.lastname: Saito
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authors.lastname: Kusajima
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authors.lastname: Kitagawa
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authors.lastname: Sugiyama
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authors.lastname: Kobayashi