title: DcHsp17.7, a small heat shock protein in carrot, is tissue-specifically expressed under salt stress and confers tolerance to salinity.
abstract: The expression and function of DcHsp17.7, a small heat shock protein in carrot (Daucus carota L.), were examined under salt stress, which is an exacerbating environmental condition due to water shortage and irrigation. DcHsp17.7 was constitutively expressed in leaf and stem tissues under normal growth conditions. Upon exposure to 300 mM NaCl, the protein level of DcHsp17.7 increased dramatically in leaf tissue, but did not significantly change in stem tissue. Native-PAGE analysis showed tissue-specific oligomer formation. Under normal growth conditions, DcHsp17.7 was found in an approximately 240 kDa complex in both tissues. However, NaCl treatment induced an additional approximately 160 kDa complex containing DcHsp17.7. This occurred only in leaf tissue, suggesting tissue-specific oligomeric complex formation. To examine the functional mechanism of DcHsp17.7 under stress conditions, the DcHsp17.7 coding gene was introduced into Escherichia coli. Heterologous expression of DcHsp17.7 was induced by isopropyl β-d-1-thiogalactopyranoside treatment. Upon exposure to salinity, protein levels of DcHsp17.7 decreased, and the protein was not detected after 16 hours. Native-PAGE analysis showed that DcHsp17.7 was present in an approximately 250 kDa complex both before and after salt treatment. Salinity reduced bacterial cell viability; however, the transgenic E. coli expressing DcHsp17.7 exhibited a higher survival rate than control E. coli under salt stress. When the level of soluble proteins was measured under salt stress, transgenic E. coli expressing DcHsp17.7 reproducibly showed slightly higher levels than control cells. This suggests that DcHsp17.7 performs molecular chaperone activity in salt-stressed transgenic E. coli. Our results suggest that DcHsp17.7 is likely to be involved in tolerance not only to thermal stresses but also to other abiotic stresses, such as salinity.
authors.affiliation: Department of Green Life Science, College of Convergence, Sangmyung University, 7 Hongji-dong, Jongno-gu, Seoul 110-743, Republic of Korea.
authors.forename: Na-Hyun
authors.initials: NH
authors.lastname: Song
authors.forename: Yeh-Jin
authors.initials: YJ
authors.lastname: Ahn
mesh_terms: D018552:Daucus carota / Q000235:genetics / Q000378:metabolism*; D024881:Drug Resistance, Bacterial / Q000187:drug effects / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant / Q000187:drug effects* / Q000502:physiology; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D054712:Salinity; D012965:Sodium Chloride / Q000494:pharmacology*; D013312:Stress, Physiological / Q000187:drug effects*
chemical_list: D006360:Heat-Shock Proteins; D010940:Plant Proteins; D011994:Recombinant Proteins; D012965:Sodium Chloride