title: Comparative antioxidant activity of edible Japanese brown seaweeds.
abstract: Japanese edible brown seaweeds, Eisenia bicyclis (Arame), Kjellmaniella crassifolia (Gagome), Alaria crassifolia (Chigaiso), Sargassum horneri (Akamoku), and Cystoseira hakodatensis (Uganomoku) were assayed for total phenolic content (TPC), fucoxanthin content, radical scavenging activities (DPPH, peroxyl radical, ABTS, and nitric oxide), and antioxidant activity in a liposome system. Among the solvents used for extraction, methanol was the most effective to extract total phenolics (TPC) from brown seaweeds. Among 5 kinds of brown seaweeds analyzed, methanol extract from C. hakodatensis was the best source for antioxidants. The high antioxidant activity of the extract was based not only on the high content of phenolics, but on the presence of fucoxanthin. No significant correlation (P > 0.05) was observed between TPC per gram extract with DPPH radical scavenging activity of the methanol extracts. These observed discrepancy would be due to structural variations in the phenolic compounds, and different levels of fucoxanthin in the extracts. The present study also demonstrated the synergy in the antioxidant activity of the combination of brown seaweed phenolics and fucoxanthin.
authors.affiliation: Lab. of Biofunctional Material Chemistry, Faculty of Fisheries Sciences, Hokkaido Univ. Minato-cho 3-1-1, Hakodate, Hokkaido 041-8611, Japan. airanthi@yahoo.com
authors.forename: M K Widjaja-Adhi
authors.initials: MK
authors.lastname: Airanthi
authors.forename: Masashi
authors.initials: M
authors.lastname: Hosokawa
authors.forename: Kazuo
authors.initials: K
authors.lastname: Miyashita
mesh_terms: D000465:Algorithms; D000975:Antioxidants / Q000032:analysis* / Q000737:chemistry* / Q000302:isolation & purification; D045424:Complex Mixtures / Q000737:chemistry / Q000302:isolation & purification; D005520:Food Preservatives / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D016166:Free Radical Scavengers / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D007564:Japan; D007795:Laminaria / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry; D000432:Methanol / Q000737:chemistry; D010084:Oxidation-Reduction; D010636:Phenols / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D010713:Phosphatidylcholines / Q000737:chemistry; D044647:Sargassum / Q000737:chemistry; D012624:Seaweed / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D013045:Species Specificity; D013997:Time Factors; D024341:Xanthophylls / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D000975:Antioxidants; D045424:Complex Mixtures; D005520:Food Preservatives; D016166:Free Radical Scavengers; D008081:Liposomes; D010636:Phenols; D010713:Phosphatidylcholines; D012997:Solvents; D024341:Xanthophylls; C025164:fucoxanthin; D000432:Methanol