title: Extraction and normal-phase HPLC-fluorescence-electrospray MS characterization and quantification of procyanidins in cranberry extracts.
abstract: Cranberries are a major source of procyanidins, an abundant class of bioactive polyphenols found in nature. The objective of this study was to apply a protocol for extraction and normal-phase high-performance liquid chromatograph-fluorescence-mass spectroscopy separation and quantification of procyanidins in cranberry extracts containing varying processing aids and/or methods of production. Cranberry extracts were best extracted using an acetone/water technique versus an acid/alkaline extraction. Procyanidins were semi-purified with C-18 and Sephadex LH-20 SPE cartridges (GE Healthcare Life Sciences, Piscataway, N.J., U.S.A.). Characterization and quantification of procyanidins up to octamers and higher molecular weight compounds, including separation of the A- and B-type dimers to tetramers was achieved.
authors.affiliation: Dept. of Food Science and Technology, Ohio State Univ., 2015 Fyffe Road, Columbus, OH 43210, USA.
authors.forename: Taylor C
authors.initials: TC
authors.lastname: Wallace
authors.forename: M Monica
authors.initials: MM
authors.lastname: Giusti
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005638:Fruit / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification*; D011108:Polymers / Q000032:analysis / Q000737:chemistry; D044945:Proanthocyanidins / Q000032:analysis* / Q000737:chemistry; D052616:Solid Phase Extraction / Q000379:methods; D013050:Spectrometry, Fluorescence; D021241:Spectrometry, Mass, Electrospray Ionization; D029799:Vaccinium macrocarpon / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D011108:Polymers; D044945:Proanthocyanidins