title: Markers of human T lymphotropic virus type I in patients with cancer of uterine cervix in Amazon, Brazil.
abstract: Human T lymphotropic virus type I(HTLV-I) has been implicated in various human diseases. Serum samples of 390 Brazilian Amazonians with cancer of various types were tested for HTLV-I antibodies by Gelatin particle agglutination test, Enzyme linked immunosorbent assay and Western blotting. Of 134 sera from patients with cancer of uterine cervix, 4 were positive by all the methods. Three of these were from non-transfused patients. DNA was extracted from 2 of 4 seropositive sera that gave strong reactions and were analyzed by PCR-SSCP for HTLV-I sequences. One was positive for all HTLV-I genes tested while the other one was positive for LTR and tax and negative for gag. In view of a possible pathway of the virus by sexual contact, the involvement of HTLV-I in cervical cancer warrants further studies.
authors.affiliation: FUNDACAO UNIV FED AMAPA,MACAPA,AMAPA,BRAZIL. CHIBA CANC CTR,RES INST,CHUO KU,CHIBA 260,JAPAN. UNIV AUTONOMA NUEVO LEON,FAC CIENCIAS BIOL,MEXICO CITY,DF,MEXICO. CALIF DEPT HLTH SERV,BERKELEY,CA 94704. EMPRESA BRASILEIRA BIOTECNOL,SAO PAULO,BRAZIL. HOSP OFFIR LOYOLA,BELEM,PARA,BRAZIL.
authors.forename: L
authors.initials: L
authors.lastname: Kanzaki
authors.forename: K
authors.initials: K
authors.lastname: Maruyama
authors.forename: T
authors.initials: T
authors.lastname: Fukushima
authors.forename: R
authors.initials: R
authors.lastname: Tamezguerra
authors.forename: L
authors.initials: L
authors.lastname: Trejoavila
authors.forename: J
authors.initials: J
authors.lastname: Casseb
authors.forename: E
authors.initials: E
authors.lastname: Neitzert
authors.forename: J
authors.initials: J
authors.lastname: Macedo