title: Fetal adrenal development: comparing effects of combined exposures to PCB 118 and PCB 153 in a sheep model.
abstract: This study investigated the effects of exposure to the ubiquitous contaminants polychlorinated biphenyls (PCBs) on the fetal adrenal cortex and on plasma cortisol using the domestic sheep (Ovis aries) as a model. Pregnant ewes were intendedly subjected to oral treatment with PCB 153 (98 μg/kg bw/day), PCB 118 (49 μg/kg bw/day) or the vehicle corn oil from mating until euthanasia on gestation day 134 (±0.25 SE). However, because of accidental cross-contamination occurring twice causing a mixed exposure scenario in all three groups, the focus of this paper is to compare three distinct groups of fetuses with different adipose tissue PCB levels (PCB 153high, PCB 118high and low, combined groups) rather than comparing animals exposed to single PCB congeners to those of a control group. When comparing endocrine and anatomical parameters from fetuses in the PCB 153high (n = 13) or PCB 118high (n = 14) groups with the low, combined group (n = 14), there was a significant decrease in fetal body weight (P < 0.05), plasma cortisol concentration (P < 0.001) and adrenal cortex thickness (P < 0.001). Furthermore, adrenal weight was decreased and plasma ACTH was increased only in the PCB 118high group. Expression of several genes encoding enzymes and receptors related to steroid hormone synthesis was also affected and mostly down-regulated in fetuses with high PCB tissue levels. In conclusion, we suggest that mono-and di-ortho PCBs were able to interfere with growth, adrenal development and cortisol production in the fetal sheep model. © 2011 Wiley Periodicals, Inc. Environ Toxicol, 2013.
authors.affiliation: Department of Basic Sciences and Aquatic Medicine, Norwegian School of Veterinary Science, Postboks 8146 Dep, 0033 Oslo, Norway. Karin.Zimmer@nvh.no
authors.forename: Karin E
authors.initials: KE
authors.lastname: Zimmer
authors.forename: Marianne
authors.initials: M
authors.lastname: Kraugerud
authors.forename: Mona
authors.initials: M
authors.lastname: Aleksandersen
authors.forename: Arno C
authors.initials: AC
authors.lastname: Gutleb
authors.forename: Gunn C
authors.initials: GC
authors.lastname: Østby
authors.forename: Ellen
authors.initials: E
authors.lastname: Dahl
authors.forename: Vidar
authors.initials: V
authors.lastname: Berg
authors.forename: Janneche U
authors.initials: JU
authors.lastname: Skaare
authors.forename: Ingrid
authors.initials: I
authors.lastname: Olsaker
authors.forename: Erik
authors.initials: E
authors.lastname: Ropstad
mesh_terms: D000311:Adrenal Glands / Q000187:drug effects* / Q000196:embryology* / Q000378:metabolism; D000818:Animals; D005260:Female; D008297:Male; D023421:Models, Animal; D011078:Polychlorinated Biphenyls / Q000633:toxicity*; D011247:Pregnancy; D012756:Sheep; D034561:Sheep, Domestic
chemical_list: C070055:2,3',4,4',5-pentachlorobiphenyl; D011078:Polychlorinated Biphenyls; C014024:2,4,5,2',4',5'-hexachlorobiphenyl