title: Restoration of motor control and dopaminergic activity in rats with unilateral 6-hydroxy-dopamine lesions.
abstract: OBJECTIVE
  To restore motor function and dopaminergic activity in the nigrostriatum of rats with unilateral 6-hydroxy-dopamine lesions using implants of encapsulated porcine choroid plexus cells.
  
  
  METHODS
  Neonatal porcine choroid plexus cells were prepared and maintained in culture, then encapsulated within alginate-polyornithine capsules (600-660 µm). Rats were unilaterally injected with 6-hydroxy-dopamine into the striatum. Those with lesions verified after 2 and 4 weeks were selected for experiments. Rats were implanted adjacent to the lesion with ten capsules 2-4 days later with (treated, n = 12) or without (control, n = 10) choroid plexus cells.
  
  
  RESULTS
  The choroid plexus cells were shown to produce a wide range of neurorestorative proteins. The treated group had a 60% improvement in motor behavior compared with the control group (p < 0.01). The treated group also had a significant improvement in nigrostriatal dopaminergic activity (31%, p < 0.02).
  
  
  CONCLUSIONS
  Capsules containing porcine choroid plexus cells release therapeutic molecules that stimulate regeneration of the lesioned nigrostriatum in rats.
authors.affiliation: Living Cell Technologies (NZ) Ltd, PO Box 23566, Hunters Corner, Papatoetoe, Auckland, New Zealand. sskinner@lctglobal.com
authors.forename: Stephen J M
authors.initials: SJ
authors.lastname: Skinner
authors.forename: Hai
authors.initials: H
authors.lastname: Lin
authors.forename: Marilyn S
authors.initials: MS
authors.lastname: Geaney
authors.forename: Thorsten
authors.initials: T
authors.lastname: Gorba
authors.forename: Robert B
authors.initials: RB
authors.lastname: Elliott
authors.forename: Paul L J
authors.initials: PL
authors.lastname: Tan
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D017690:Cell Transplantation; D002831:Choroid Plexus / Q000166:cytology; D007150:Immunohistochemistry; D020007:Implants, Experimental; D009043:Motor Activity / Q000502:physiology*; D017072:Neostriatum / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology*; D016627:Oxidopamine; D051381:Rats; D020127:Recovery of Function / Q000502:physiology*; D034421:Sus scrofa; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: D016627:Oxidopamine; D014446:Tyrosine 3-Monooxygenase