title: Improved adhesion of human cultured periosteal sheets to a porous poly(L-lactic acid) membrane scaffold without the aid of exogenous adhesion biomolecules.
abstract: Human cultured periosteal sheets, which are developed from small excised periosteum tissue segments (PTSs) in culture dishes by simple expansion culture, have been applied as a promising autologous osteogenic grafting material for periodontal regenerative therapy. However, the weak initial adhesion of PTSs to dish surfaces often hampers cellular outgrowth and limits the number of preparations. To correct this weakness and still avoid the use of animal-derived adhesion biomolecules, we have developed a novel, biodegradable, porous poly(L-lactic acid) (pPLLA) membrane. Freshly excised PTSs bound well to the highly porous pPLLA membrane, possibly due to the presence of semihemispheric 20-30 μm diameter openings on the upper surface. Global gene expression analysis demonstrated that periosteal sheets cultured on pPLLA membranes upregulated expression of many adhesion molecules. Osteogenic induction stimulated the production of proteoglycans by these cells and concomitantly enhanced their expansion and penetration into the deep pore regions of the membrane in parallel with the progression of in vitro mineralization. These findings suggest that our pPLLA membranes not only facilitate initial adhesion, primarily mediated by adsorbed proteins, but also enhance biological adhesion by inducing endogenous adhesion molecules in periosteal sheet cultures. Therefore, the efficacy of periosteal sheets in therapy should be greatly enhanced by using this new pPLLA membrane.
authors.affiliation: Division of Oral Bioengineering, Department of Tissue Regeneration and Reconstitution, Institute of Medicine and Dentistry, Niigata University, Niigata 951-8514, Japan. kawase@dent.niigata-u.ac.jp
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Kawase
authors.forename: Takaaki
authors.initials: T
authors.lastname: Tanaka
authors.forename: Takayuki
authors.initials: T
authors.lastname: Nishimoto
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Okuda
authors.forename: Masaki
authors.initials: M
authors.lastname: Nagata
authors.forename: Douglas M
authors.initials: DM
authors.lastname: Burns
authors.forename: Hiromasa
authors.initials: H
authors.lastname: Yoshie
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects; D015815:Cell Adhesion Molecules / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D019344:Lactic Acid / Q000494:pharmacology*; D008855:Microscopy, Electron, Scanning; D010521:Periosteum / Q000166:cytology* / Q000187:drug effects* / Q000201:enzymology; D011091:Polyesters; D011108:Polymers / Q000494:pharmacology*; D016062:Porosity / Q000187:drug effects; D011509:Proteoglycans / Q000235:genetics / Q000378:metabolism; D013997:Time Factors; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D015815:Cell Adhesion Molecules; D011091:Polyesters; D011108:Polymers; D011509:Proteoglycans; D019344:Lactic Acid; C033616:poly(lactide); D000469:Alkaline Phosphatase