title: [Detection of tick-borne encephalitis virus in Ixodes ticks collected in a natural focus of Gornyi Altai].
abstract: Enzyme immunoassay of tick-borne encephalitis virus (TBE) in the samples of Ixodes ticks collected in the outskirts of the settlement of Manzherok, Maiminsk District, Republic of Altai, revealed TBE antigen in 16.9 +/- 1.9% of the talga ticks. Real-time reverse transcription-polymerase chain reaction (RT-PCR) with specific fluorescent probes and phylogenetic analysis of the nucleotide sequences of RT-RCR products corresponding to 5'-terminal fragment of the E gene of TBE, all the virus strains isolated from the ticks collected in Gomyi Altai were referred to as the Siberian genetic type that was dominant in virus-carrying ticks in the majority of endemic areas of Russia and near abroad. Viral load assays using the real-time RT-PCR with the probes indicated the threshold cycles Ct = 25.34-28.98, which, with regard to the efficiency of RNA identification and reverse transcription, was equal to about 10(4)-10(5) viral RNA copies per tick.
authors.forename: O V
authors.initials: OV
authors.lastname: Morozova
authors.forename: A E
authors.initials: AE
authors.lastname: Grishechkin
authors.forename: L S
authors.initials: LS
authors.lastname: Karan
authors.forename: E I
authors.initials: EI
authors.lastname: Isayeva
authors.forename: L D
authors.initials: LD
authors.lastname: Shchuchinova
authors.forename: N V
authors.initials: NV
authors.lastname: Loginova
authors.forename: V I
authors.initials: VI
authors.lastname: Zlobin
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004669:Encephalitis Viruses, Tick-Borne / Q000235:genetics / Q000302:isolation & purification*; D004675:Encephalitis, Tick-Borne / Q000453:epidemiology / Q000235:genetics / Q000821:virology*; D004797:Enzyme-Linked Immunosorbent Assay; D005814:Genes, Viral; D005838:Genotype; D006801:Humans; D018884:Ixodes / Q000235:genetics / Q000821:virology*; D008969:Molecular Sequence Data; D015995:Prevalence; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012426:Russia / Q000453:epidemiology; D012621:Seasons; D019562:Viral Load
chemical_list: D012367:RNA, Viral