title: Characterization and purification of angiogenic factor derived from highly liver metastatic colon cancer cells.
abstract: We investigated the effects of serum-free conditioned medium (SFCM) of colon cancer cells on human umbilical vein endothelial cells (HUVECs). SFCM of highly liver metastatic cell line (LM-HS) strongly enhanced proliferation, migration and tube formation of HUVECs. Moreover, it was suggested that a main angiogenic factor derived from LM-H5 may be VEGF based on HPLC, SDS-PAGE analysis and neutralizing experiment using anti-growth factor antibodies. These findings suggest that angiogenesis is related to the processes of metastasis of colon cancer, and that VEGF synthesized by colon cancer cells acts as a key factor inducing angiogenesis.
authors.affiliation: OSAKA CITY UNIV,SCH MED,DEPT SURG 1,ABENO KU,OSAKA 545,JAPAN. OSAKA CITY UNIV,SCH MED,DEPT INTERNAL MED 3,ABENO KU,OSAKA 545,JAPAN. OSAKA CITY UNIV,SCH MED,CHEM LAB,ABENO KU,OSAKA 545,JAPAN.
authors.forename: S
authors.initials: S
authors.lastname: Takatsuka
authors.forename: Y
authors.initials: Y
authors.lastname: Chung
authors.forename: N
authors.initials: N
authors.lastname: Yamada
authors.forename: T
authors.initials: T
authors.lastname: Sawada
authors.forename: S
authors.initials: S
authors.lastname: Seki
authors.forename: Y
authors.initials: Y
authors.lastname: Funae
authors.forename: M
authors.initials: M
authors.lastname: Sowa