title: Bryocella elongata gen. nov., sp. nov., a member of subdivision 1 of the Acidobacteria isolated from a methanotrophic enrichment culture, and emended description of Edaphobacter aggregans Koch et al. 2008.
abstract: An aerobic, pink-pigmented, chemo-organotrophic bacterium, designated strain SN10(T), was isolated from a methanotrophic enrichment culture obtained from an acidic Sphagnum peat. This isolate was represented by Gram-negative, non-motile rods that multiply by normal cell division and form rosettes. Strain SN10(T) is an obligately acidophilic, mesophilic bacterium capable of growth at pH 3.2-6.6 (with an optimum at pH 4.7-5.2) and at 6-32 °C (with an optimum at 20-24 °C). The preferred growth substrates are sugars and several heteropolysaccharides of plant and microbial origin, such as pectin, lichenan, fucoidan and gellan gum. While not being capable of growth on C(1) compounds, strain SN10(T) can develop in co-culture with exopolysaccharide-producing methanotrophs by utilization of their capsular material. The major fatty acids determined in strain SN10(T) using the conventional lipid extraction procedure are iso-C(15:0) and C(16:1)ω7c. Upon hydrolysis of total cell material, substantial amounts of the uncommon membrane-spanning lipid 13,16-dimethyl octacosanedioic acid (isodiabolic acid) were also detected. The polar lipids are two phosphohexoses, phosphatidylethanolamine, phosphatidylglycerol and several phospholipids of unknown structure. The major quinone is MK-8. Pigments are carotenoids. The G+C content of the DNA is 60.7 mol%. Strain SN10(T) forms a separate lineage within subdivision 1 of the phylum Acidobacteria and displays 94.0-95.4% 16S rRNA gene sequence similarity to members of the genera Edaphobacter and Granulicella, 93.0-93.7% similarity to members of the genus Terriglobus and 92.2-92.3 % similarity to the type strains of Telmatobacter bradus and Acidobacterium capsulatum. Therefore, strain SN10(T) is classified within a novel genus and species, for which the name Bryocella elongata gen. nov., sp. nov. is proposed. Strain SN10(T) (=LMG 25276(T) =DSM 22489(T)) is the type strain of Bryocella elongata. An emended description of Edaphobacter aggregans Koch et al. 2008 is also given.
authors.affiliation: S. N. Winogradsky Institute of Microbiology, Russian Academy of Sciences, Moscow 117312, Russia.
authors.forename: Svetlana N
authors.initials: SN
authors.lastname: Dedysh
authors.affiliation: S. N. Winogradsky Institute of Microbiology, Russian Academy of Sciences, Moscow 117312, Russia.
authors.forename: Irina S
authors.initials: IS
authors.lastname: Kulichevskaya
authors.affiliation: S. N. Winogradsky Institute of Microbiology, Russian Academy of Sciences, Moscow 117312, Russia.
authors.forename: Yulia M
authors.initials: YM
authors.lastname: Serkebaeva
authors.affiliation: S. N. Winogradsky Institute of Microbiology, Russian Academy of Sciences, Moscow 117312, Russia.
authors.forename: Maria A
authors.initials: MA
authors.lastname: Mityaeva
authors.affiliation: S. N. Winogradsky Institute of Microbiology, Russian Academy of Sciences, Moscow 117312, Russia.
authors.forename: Vladimir V
authors.initials: VV
authors.lastname: Sorokin
authors.affiliation: G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region 142292, Russia.
authors.forename: Natalia E
authors.initials: NE
authors.lastname: Suzina
authors.affiliation: NIOZ Royal Netherlands Institute for Sea Research, Department of Marine Organic Biogeochemistry, PO Box 59, 1790 AB Den Burg, The Netherlands.
authors.forename: W Irene C
authors.initials: WIC
authors.lastname: Rijpstra
authors.affiliation: NIOZ Royal Netherlands Institute for Sea Research, Department of Marine Organic Biogeochemistry, PO Box 59, 1790 AB Den Burg, The Netherlands.
authors.forename: Jaap S
authors.initials: JS
authors.lastname: Sinninghe Damsté
mesh_terms: D061271:Acidobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D000332:Aerobiosis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D016227:Benzoquinones / Q000032:analysis; D050260:Carbohydrate Metabolism; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008853:Microscopy; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D010860:Pigments, Biological / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D044003:Sphagnopsida / Q000382:microbiology*; D013696:Temperature
chemical_list: D016227:Benzoquinones; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D010860:Pigments, Biological; D012336:RNA, Ribosomal, 16S; C004532:quinone