title: Synthesis of fluorescently labelled rhamnosides: probes for the evaluation of rhamnogalacturonan II biosynthetic enzymes.
abstract: Three fluorescently labelled saccharides 10-12, representing structures found in pectic glycan rhamnogalacturonan II (RG-II), were synthesised by chemical glycosylation of O-6 of diacetone-d-galactose followed by deprotection and reductive amination with amino-substituted fluorophore APTS. This convenient method installs a common aminogalactitol-based tether in order to preserve the integrity of the reducing end of specific carbohydrates of interest. APTS-labelled glycans prepared in this manner were purified by carbohydrate gel electrophoresis and subjected to capillary electrophoresis analysis, as a basis for the subsequent development of high sensitivity assays for RG-II-active enzymes.
authors.affiliation: Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK.
authors.forename: Efthymia
authors.initials: E
authors.lastname: Prifti
authors.forename: Stephan
authors.initials: S
authors.lastname: Goetz
authors.forename: Sergey A
authors.initials: SA
authors.lastname: Nepogodiev
authors.forename: Robert A
authors.initials: RA
authors.lastname: Field
mesh_terms: D019075:Electrophoresis, Capillary; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D005690:Galactose / Q000737:chemistry*; D006031:Glycosylation; D010084:Oxidation-Reduction; D010368:Pectins / Q000737:chemistry*; D010940:Plant Proteins / Q000032:analysis / Q000378:metabolism; D010944:Plants / Q000737:chemistry / Q000201:enzymology; D011721:Pyrenes / Q000032:analysis / Q000737:chemistry*; D013194:Staining and Labeling / Q000379:methods*
chemical_list: C098210:1-aminopyrene-3,6,8-trisulfonic acid; D005456:Fluorescent Dyes; D010940:Plant Proteins; D011721:Pyrenes; C042492:rhamnogalacturonan II; D010368:Pectins; D005690:Galactose