title: Characterization of extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae isolates from the community in Morocco.
abstract: Of 803 community Escherichia coli (n = 767) and Klebsiella pneumoniae (n = 36) isolates collected from patients with urinary tract infections in three Moroccan cities, 10 E. coli (1.3%) and 2 K. pneumoniae (5.6 %) isolates were shown to produce extended-spectrum β-lactamases (ESBLs). PFGE revealed that the E. coli isolates comprised seven distinct genotypes. The presence of plasmids in the 12 isolates was revealed by conjugation experiments of plasmids from these Enterobacteriaceae strains with E. coli K(12)J(5), with further isolation of the plasmids in the transconjugants. Subsequent nucleotide sequencing indicated that the plasmids encoded the bla(CTX-M), bla(OXA), bla(TEM) and bla(SHV) genes, including genes for CTX-M-15 (n = 11), OXA-1 (n = 11), TEM-1b (n = 4), SHV-5 (n = 1) and SHV-1 (n = 2). Identification of plasmid-mediated quinolone-resistance genes was performed by PCR. The aac(6')Ib-cr variant was detected in all strains, and two strains co-expressed qnrS1, bla(CTX-M-15) and bla(OXA-1) genes. The presence of ESBLs in the Enterobacteriaceae strains studied was probably due to the dissemination of resistance plasmids with the predominant genotype of bla(CTX-M-15.).
authors.affiliation: Microbiology, Health and Environment Team, Department of Biology, School of Sciences, Chouaib Doukkali University, El Jadida, Morocco.|Molecular Bacteriology Laboratory, Pasteur Institute of Morocco, Casablanca, Morocco.
authors.forename: Abouddihaj
authors.initials: A
authors.lastname: Barguigua
authors.affiliation: Microbiology, Health and Environment Team, Department of Biology, School of Sciences, Chouaib Doukkali University, El Jadida, Morocco.
authors.forename: Fatima
authors.initials: F
authors.lastname: El Otmani
authors.affiliation: Microbiology, Health and Environment Team, Department of Biology, School of Sciences, Chouaib Doukkali University, El Jadida, Morocco.
authors.forename: Mustapha
authors.initials: M
authors.lastname: Talmi
authors.affiliation: Molecular Bacteriology Laboratory, Pasteur Institute of Morocco, Casablanca, Morocco.
authors.forename: Fatna
authors.initials: F
authors.lastname: Bourjilat
authors.affiliation: Microbiology, Health and Environment Team, Department of Biology, School of Sciences, Chouaib Doukkali University, El Jadida, Morocco.|Molecular Bacteriology Laboratory, Pasteur Institute of Morocco, Casablanca, Morocco.
authors.forename: Fatima
authors.initials: F
authors.lastname: Haouzane
authors.affiliation: University Hospital Center, Ibn Rochd, Casablanca, Morocco.
authors.forename: Khalid
authors.initials: K
authors.lastname: Zerouali
authors.affiliation: Molecular Bacteriology Laboratory, Pasteur Institute of Morocco, Casablanca, Morocco.
authors.forename: Mohammed
authors.initials: M
authors.lastname: Timinouni
mesh_terms: D016000:Cluster Analysis; D017714:Community-Acquired Infections / Q000382:microbiology*; D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D004926:Escherichia coli / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000382:microbiology*; D005798:Genes, Bacterial; D005838:Genotype; D006801:Humans; D007710:Klebsiella Infections / Q000382:microbiology*; D007711:Klebsiella pneumoniae / Q000201:enzymology* / Q000302:isolation & purification; D058889:Molecular Typing; D009018:Morocco; D010957:Plasmids / Q000032:analysis; D017422:Sequence Analysis, DNA; D014552:Urinary Tract Infections / Q000382:microbiology; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D001618:beta-Lactamases