title: [Effects of a microfluidic sperm sorter on sperm routine parameters and DNA integrity].
abstract: OBJECTIVE
  To investigate the effects of a microfluidic sperm sorter on the routine parameters and DNA integrity of human sperm.
  
  
  METHODS
  We divided 40 semen samples into two aliquots and performed sperm sorting using a self-made polydimethylsiloxane microfluidic sperm sorter and the swim-up method, respectively. Then we evaluated and compared the effects of these two methods on the sperm routine parameters and DNA integrity by computer-assisted sperm analysis and sperm chromatin dispersion test.
  
  
  RESULTS
  After processing, sperm motility, normal morphology and tail hypoosmotic swelling rate were significantly improved, while sperm DNA damage remarkably decreased (P < 0.01). The microfluidic sperm sorter achieved a significantly lower rate of sperm DNA damage than the swim-up method ([ 8.4 +/- 5.8 ]% vs [16.4 +/- 9.2] %, P < 0.01), but no statistically significant differences were found in all other parameters between the two methods.
  
  
  CONCLUSIONS
  High-quality sperm with less DNA integrity damage could be obtained in sperm sorting with the microfluidic sperm sorter.
authors.affiliation: Department of Clinical Laboratory, The First People's Hospital of Guangzhou Affiliated to Guangzhou Medical College, Guangzhou, Guangdong 510180, China. wwbrain@163.com
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Guang-Tie
authors.initials: GT
authors.lastname: Liang
authors.forename: Ya-Ya
authors.initials: YY
authors.lastname: Peng
authors.forename: Da-Yu
authors.initials: DY
authors.lastname: Liu
authors.forename: Xiao-Mian
authors.initials: XM
authors.lastname: Zhou
mesh_terms: D000328:Adult; D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods; D004247:DNA; D004249:DNA Damage; D006801:Humans; D007248:Infertility, Male / Q000235:genetics / Q000503:physiopathology; D008297:Male; D044085:Microfluidics; D008875:Middle Aged; D040081:Protein Array Analysis; D055101:Semen Analysis / Q000295:instrumentation* / Q000379:methods; D013081:Sperm Motility; D013094:Spermatozoa
chemical_list: D004247:DNA