title: The seroprevalence of Lawsonia intracellularis in horses in The Netherlands.
abstract: Equine proliferative enteropathy caused by Lawsonia intracellularis is an emerging disease of weanling foals and affects their growth and development. The prevalence of Lawsonia intracellularis in The Netherlands is not known. The aim of the study was to investigate the seroprevalence of Lawsonia intracellularis in horses in The Netherlands. Blood samples were taken from healthy foals before and after weaning and from healthy yearlings and mature horses on farms throughout The Netherlands. These samples were analysed for the presence of Lawsonia intracellularis-specific antibodies with a blocking ELISA. White blood cell count, packed cell volume, and total protein concentration were also measured in all foals. Information regarding housing, pasture access, and contact with pig manure on the premises was obtained for all animals. The prevalence of Lawsonia intracellularis antibodies in foals increased significantly from 15% before weaning to 23% after weaning (p = 0.019); it was 89% in yearlings and 99% in horses older than 2 years. There was no significant difference in seroprevalence between the pasture-kept and stable-confined adult horses (97% and 100%, respectively), and there was no significant influence of contact with pig manure. None of the sampled animals showed clinical disease. In conclusion, the results suggest that Lawsonia intracellularis is widespread in The Netherlands and that seropositivity is not necessarily associated with clinical problems. The high seroprevalence in adult horses suggests long-term persistence of antibodies against Lawsonia intracellularis or constant exposure to the bacterium.
authors.affiliation: Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 114, 3584 CM, Utrecht, The Netherlands.
authors.forename: L C
authors.initials: LC
authors.lastname: Kranenburg
authors.forename: H E M I
authors.initials: HE
authors.lastname: van Ree
authors.forename: A N M
authors.initials: AN
authors.lastname: Calis
authors.forename: M
authors.initials: M
authors.lastname: de Pater
authors.forename: G J
authors.initials: GJ
authors.lastname: Buter
authors.forename: C
authors.initials: C
authors.lastname: van Maanen
authors.forename: M M
authors.initials: MM
authors.lastname: Sloet van Oldruitenborgh-Oosterbaarn
mesh_terms: D000822:Animal Husbandry / Q000379:methods / Q000706:statistics & numerical data; D000818:Animals; D000831:Animals, Newborn; D000907:Antibodies, Bacterial / Q000097:blood*; D045824:Desulfovibrionaceae Infections / Q000453:epidemiology / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D006734:Horse Diseases / Q000453:epidemiology*; D006736:Horses; D020639:Lawsonia Bacteria / Q000276:immunology*; D008297:Male; D009426:Netherlands / Q000453:epidemiology; D016036:Seroepidemiologic Studies; D014886:Weaning
chemical_list: D000907:Antibodies, Bacterial