title: Multiplex polymerase chain reaction used for bovine embryo sex determination.
abstract: Widely used bovine sexing primers were compared in terms of suitability in determining the sex of bovine embryos. Under optimized multiplex PCR conditions, the ConBV/ConEY couple primers did not show accurate results when combined together in multiplex PCR, but worked well when the couple primers were used separately. The S4BF/S4BR primers showed accurate results; however, some unexpected bands were detected. When the BY/BSP couple primers were used to determine one-cell, two-cell, four-cell and eight-cell stage embryos of known sexed SCNT-derived embryos, the results showed 100% accuracy. The BY/BSP couple primers were also able to identify the sex of one-cell and two-cell IVF-derived embryos.
authors.affiliation: Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima, Thailand.
authors.forename: Surachai
authors.initials: S
authors.lastname: Rattanasuk
authors.forename: Rangsun
authors.initials: R
authors.lastname: Parnpai
authors.forename: Mariena
authors.initials: M
authors.lastname: Ketudat-Cairns
mesh_terms: D000818:Animals; D002417:Cattle; D017931:DNA Primers / Q000235:genetics; D004624:Embryo Transfer / Q000379:methods; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods; D008297:Male; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA; D012732:Sex Determination Analysis / Q000379:methods*
chemical_list: D017931:DNA Primers