title: A biomimetic membrane device that modulates the excessive inflammatory response to sepsis.
abstract: OBJECTIVE
  Septic shock has a clinical mortality rate approaching fifty percent. The major clinical manifestations of sepsis are due to the dysregulation of the host's response to infection rather than the direct consequences of the invading pathogen. Central to this initial immunologic response is the activation of leukocytes and microvascular endothelium resulting in cardiovascular instability, lung injury and renal dysfunction. Due to the primary role of leukocyte activation in the sepsis syndrome, a synthetic biomimetic membrane, called a selective cytopheretic device (SCD), was developed to bind activated leukocytes. The incorporation of the SCD along an extracorporeal blood circuit coupled with regional anticoagulation with citrate to lower blood ionized calcium was devised to modulate leukocyte activation in sepsis.
  
  
  METHODS
  Laboratory investigation.
  
  
  METHODS
  University of Michigan Medical School.
  
  
  METHODS
  Pigs weighing 30-35 kg.
  
  
  METHODS
  To assess the effect of the SCD in septic shock, pigs were administered 30×10(10) bacteria/kg body weight of Escherichia coli into the peritoneal cavity and within 1 hr were immediately placed in an extracorporeal circuit containing SCD.
  
  
  RESULTS
  In this animal model, the SCD with citrate compared to control groups without the SCD or with heparin anticoagulation ameliorated the cardiovascular instability and lung sequestration of activated leukocytes, reduced renal dysfunction and improved survival time compared to various control groups. This effect was associated with minimal elevations of systemic circulating neutrophil activation.
  
  
  CONCLUSIONS
  These preclinical studies along with two favorable exploratory clinical trials form the basis of an FDA-approved investigational device exemption for a pivotal multicenter, randomized control trial currently underway.
authors.affiliation: Division of Nephrology, Huashan Hospital, Fudan University, Shanghai, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Ding
authors.forename: Joon Ho
authors.initials: JH
authors.lastname: Song
authors.forename: Ju Young
authors.initials: JY
authors.lastname: Jung
authors.forename: Liandi
authors.initials: L
authors.lastname: Lou
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.forename: Linda
authors.initials: L
authors.lastname: Charles
authors.forename: Angela
authors.initials: A
authors.lastname: Westover
authors.forename: Peter L
authors.initials: PL
authors.lastname: Smith
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Pino
authors.forename: Deborah A
authors.initials: DA
authors.lastname: Buffington
authors.forename: H David
authors.initials: HD
authors.lastname: Humes
mesh_terms: D000818:Animals; D007150:Immunohistochemistry; D007249:Inflammation / Q000517:prevention & control*; D008567:Membranes, Artificial; D018716:Molecular Mimicry; D018805:Sepsis / Q000517:prevention & control*; D013552:Swine
chemical_list: D008567:Membranes, Artificial