title: Acid-degradable cationic dextran particles for the delivery of siRNA therapeutics.
abstract: We report a new acid-sensitive, biocompatible, and biodegradable microparticulate delivery system, spermine modified acetalated-dextran (Spermine-Ac-DEX), which can be used to efficiently encapsulate siRNA. These particles demonstrated efficient gene knockdown in HeLa-luc cells with minimal toxicity. This knockdown was comparable to that obtained using Lipofectamine, a commercially available transfection reagent generally limited to in vitro use due to its high toxicity.
authors.affiliation: College of Chemistry, University of California, Berkeley, California 94720-1460, United States.
authors.forename: Jessica L
authors.initials: JL
authors.lastname: Cohen
authors.forename: Stephanie
authors.initials: S
authors.lastname: Schubert
authors.forename: Peter R
authors.initials: PR
authors.lastname: Wich
authors.forename: Lina
authors.initials: L
authors.lastname: Cui
authors.forename: Joel A
authors.initials: JA
authors.lastname: Cohen
authors.forename: Justin L
authors.initials: JL
authors.lastname: Mynar
authors.forename: Jean M J
authors.initials: JM
authors.lastname: Fréchet
mesh_terms: D000143:Acids / Q000737:chemistry*; D002236:Carbohydrate Conformation; D002412:Cations / Q000138:chemical synthesis / Q000737:chemistry; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003911:Dextrans / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D016503:Drug Delivery Systems / Q000379:methods*; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010316:Particle Size; D034741:RNA, Small Interfering / Q000008:administration & dosage* / Q000494:pharmacology; D013329:Structure-Activity Relationship; D013499:Surface Properties; D014162:Transfection / Q000379:methods*
chemical_list: D000143:Acids; D002412:Cations; D003911:Dextrans; D034741:RNA, Small Interfering