title: Influence of dietary advanced glycation end products on wound healing in nondiabetic mice.
abstract: The present study was to determine advanced glycation end products (AGEs) in foods from different processes, and the influence of dietary AGEs on wound healing in nondiabetic mice. AGEs mixtures were extracted from local fast foods and foods prepared in lab. A BSA-AGEs mixture made by incubating glucose with bovine serum albumin (BSA) was used as a positive control. Burns were made on the skin of mice. The results showed that foods processed by high temperatures generated higher dietary AGEs. Nonwounded mice showed no observable adverse response to high dietary AGEs. However, high dietary AGEs caused severe inflammatory responses in wounded mice. The plasma level of high mobility group box 1 (HMGB1) and its mRNA in white blood cells were found to be significantly higher in the wounded mice fed with high dietary AGEs than others. We conclude that dietary AGEs worsen inflammation and delay wound healing in nondiabetic burned mice, which might be mediated by HMGB1.
authors.affiliation: Dept. of Biochemistry and Molecular Biology, Medical School, Jinan Univ., Guangzhou, 510632, China.
authors.forename: Yuanchang
authors.initials: Y
authors.lastname: Zhu
authors.forename: Feifei
authors.initials: F
authors.lastname: Lan
authors.forename: Jiange
authors.initials: J
authors.lastname: Wei
authors.forename: Boonhor
authors.initials: B
authors.lastname: Chong
authors.forename: Pakho
authors.initials: P
authors.lastname: Chen
authors.forename: Longquan
authors.initials: L
authors.lastname: Huynh
authors.forename: Nganwa
authors.initials: N
authors.lastname: Wong
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000818:Animals; D021461:Animals, Outbred Strains; D002056:Burns / Q000276:immunology / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D003296:Cooking; D004032:Diet / Q000009:adverse effects*; D057140:Fast Foods / Q000032:analysis; D017127:Glycation End Products, Advanced / Q000032:analysis / Q000097:blood / Q000302:isolation & purification / Q000633:toxicity*; D024243:HMGB1 Protein / Q000097:blood / Q000235:genetics / Q000378:metabolism; D007962:Leukocytes / Q000378:metabolism; D008297:Male; D008460:Meat / Q000032:analysis / Q000528:radiation effects; D051379:Mice; D008872:Microwaves / Q000009:adverse effects; D012333:RNA, Messenger / Q000378:metabolism; D011897:Random Allocation; D012710:Serum Albumin, Bovine / Q000633:toxicity; D012867:Skin / Q000276:immunology / Q000293:injuries / Q000473:pathology; D013047:Specific Pathogen-Free Organisms; D016019:Survival Analysis; D014945:Wound Healing
chemical_list: D017127:Glycation End Products, Advanced; D024243:HMGB1 Protein; D012333:RNA, Messenger; C081628:advanced glycation end products-bovine serum albumin; D012710:Serum Albumin, Bovine