title: Protection of European domestic pigs from virulent African isolates of African swine fever virus by experimental immunisation.
abstract: African swine fever (ASF) is an acute haemorrhagic disease of domestic pigs for which there is currently no vaccine. We showed that experimental immunisation of pigs with the non-virulent OURT88/3 genotype I isolate from Portugal followed by the closely related virulent OURT88/1 genotype I isolate could confer protection against challenge with virulent isolates from Africa including the genotype I Benin 97/1 isolate and genotype X Uganda 1965 isolate. This immunisation strategy protected most pigs challenged with either Benin or Uganda from both disease and viraemia. Cross-protection was correlated with the ability of different ASFV isolates to stimulate immune lymphocytes from the OURT88/3 and OURT88/1 immunised pigs.
authors.affiliation: Institute for Animal Health Pirbright Laboratory, Pirbright, Woking, Surrey GU24 0NF, UK.
authors.forename: Katherine
authors.initials: K
authors.lastname: King
authors.forename: Dave
authors.initials: D
authors.lastname: Chapman
authors.forename: Jordi M
authors.initials: JM
authors.lastname: Argilaguet
authors.forename: Emma
authors.initials: E
authors.lastname: Fishbourne
authors.forename: Evelyne
authors.initials: E
authors.lastname: Hutet
authors.forename: Roland
authors.initials: R
authors.lastname: Cariolet
authors.forename: Geoff
authors.initials: G
authors.lastname: Hutchings
authors.forename: Christopher A L
authors.initials: CA
authors.lastname: Oura
authors.forename: Christopher L
authors.initials: CL
authors.lastname: Netherton
authors.forename: Katy
authors.initials: K
authors.lastname: Moffat
authors.forename: Geraldine
authors.initials: G
authors.lastname: Taylor
authors.forename: Marie-Frederique
authors.initials: MF
authors.lastname: Le Potier
authors.forename: Linda K
authors.initials: LK
authors.lastname: Dixon
authors.forename: Haru-H
authors.initials: HH
authors.lastname: Takamatsu
mesh_terms: D000357:African Swine Fever / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D000358:African Swine Fever Virus / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification / Q000472:pathogenicity*; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001541:Benin; D007114:Immunization; D007371:Interferon-gamma / Q000096:biosynthesis; D011174:Portugal; D034421:Sus scrofa / Q000276:immunology* / Q000821:virology; D013552:Swine; D013601:T-Lymphocytes / Q000276:immunology; D014454:Uganda; D014765:Viral Vaccines / Q000008:administration & dosage* / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D014765:Viral Vaccines; D007371:Interferon-gamma