title: Identification of tetrabromobisphenol A diallyl ether as an emerging neurotoxicant in environmental samples by bioassay-directed fractionation and HPLC-APCI-MS/MS.
abstract: Brominated flame retardants (BFRs) have been widely used as additives in products to reduce their flammability. Recent findings suggested that some BFRs exhibit neurotoxicity and thus might pose a threat to human health. In this work, a neurotoxicity assay-directed analysis was developed, combining sample cleanup, fractionation, chemical identification, and bioassay. Viability of primary cultured cerebellar granule neurons (CGNs) was used to evaluate the neurotoxicity of extracts or separated fractions from environmental samples. Tetrabromobisphenol A diallyl ether (TBBPA DAE) was identified as the causative toxicant in sediment samples collected from a river near a brominated flame retardant (BFR) manufacturing plant in South China. Liquid chromatography atmospheric pressure chemical ionization tandem mass spectrometry (LC-APCI-MS/MS) was optimized to determine TBBPA DAE levels in the potent fractions and to confirm TBBPA DAE as the key neurotoxicant. On the basis of comparison with the structure of other TBBPA derivatives, the 1-propenyl group in TBBPA DAE appears to be the cause for the neurotoxic potency. The levels of TBBPA DAE in samples along the river were found at up to 49 ng/L for river water, 10,183 ng/g dry weight (dw) in surface sediments, and 42 ng/g dw in soils. According to the distribution of TBBPA DAE in the environmental samples, the manufacturing plant was identified as the release source of TBBPA DAE. To our knowledge, this study is the first to demonstrate potential neurotoxicity induced by TBBPA DAE in real environmental samples.
authors.affiliation: State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
authors.forename: Guangbo
authors.initials: G
authors.lastname: Qu
authors.forename: Jianbo
authors.initials: J
authors.lastname: Shi
authors.forename: Thanh
authors.initials: T
authors.lastname: Wang
authors.forename: Jianjie
authors.initials: J
authors.lastname: Fu
authors.forename: Zhuona
authors.initials: Z
authors.lastname: Li
authors.forename: Pu
authors.initials: P
authors.lastname: Wang
authors.forename: Ting
authors.initials: T
authors.lastname: Ruan
authors.forename: Guibin
authors.initials: G
authors.lastname: Jiang
mesh_terms: D000818:Animals; D001681:Biological Assay; D002478:Cells, Cultured; D005411:Flame Retardants / Q000032:analysis / Q000633:toxicity*; D008401:Gas Chromatography-Mass Spectrometry; D019015:Geologic Sediments / Q000032:analysis; D009498:Neurotoxins / Q000302:isolation & purification*; D011075:Polybrominated Biphenyls / Q000032:analysis / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012989:Soil Pollutants / Q000032:analysis / Q000633:toxicity*
chemical_list: D005411:Flame Retardants; D009498:Neurotoxins; D011075:Polybrominated Biphenyls; D012989:Soil Pollutants; C471350:tetrabromobisphenol A-bisallylether