title: Acoustically mounted microcrystals yield high-resolution X-ray structures.
abstract: We demonstrate a general strategy for determining structures from showers of microcrystals. It uses acoustic droplet ejection to transfer 2.5 nL droplets from the surface of microcrystal slurries, through the air, onto mounting micromesh pins. Individual microcrystals are located by raster-scanning a several-micrometer X-ray beam across the cryocooled micromeshes. X-ray diffraction data sets merged from several micrometer-sized crystals are used to determine 1.8 Ǻ resolution crystal structures.
authors.affiliation: Biology Department, Brookhaven National Laboratory, Upton, NY 11973-5000, USA. soares@bnl.gov
authors.forename: Alexei S
authors.initials: AS
authors.lastname: Soares
authors.forename: Matthew A
authors.initials: MA
authors.lastname: Engel
authors.forename: Richard
authors.initials: R
authors.lastname: Stearns
authors.forename: Sammy
authors.initials: S
authors.lastname: Datwani
authors.forename: Joe
authors.initials: J
authors.lastname: Olechno
authors.forename: Richard
authors.initials: R
authors.lastname: Ellson
authors.forename: John M
authors.initials: JM
authors.lastname: Skinner
authors.forename: Marc
authors.initials: M
authors.lastname: Allaire
authors.forename: Allen M
authors.initials: AM
authors.lastname: Orville
mesh_terms: D000162:Acoustics; D018360:Crystallography, X-Ray / Q000379:methods*