title: Methylorosula polaris gen. nov., sp. nov., an aerobic, facultatively methylotrophic psychrotolerant bacterium from tundra wetland soil.
abstract: Three strains of Gram-negative, aerobic, motile bacteria with bipolar flagella were isolated from acidic tundra wetland soils near the city of Vorkuta and from the Chukotka and Yugorsky Peninsulas and designated strains V-022(T), Ch-022 and Ju-022. The cells were rod-shaped, 0.5-0.6 µm in width and 1.3-4.5 µm in length and reproduced by irregular fission. These bacteria were facultative methylotrophs that used methanol, methylamines and a wide range of other sources of carbon and energy such as sugars and polysaccharides, ethanol and amino acids. The isolates used the Calvin-Benson pathway for the assimilation of one-carbon compounds and were unable to fix nitrogen. The new strains were moderately acidophilic and psychrotolerant, capable of growth over a pH range of 4.0 to 7.8, with optimum growth at pH 5.5-6.0. Growth occurred between 4 and 30 °C (optimum 20-25 °C). The principal phospholipid fatty acid was C(18:1)ω7c. The DNA G+C content of strain V-022(T) was 65.2 mol%. Analysis of the 16S rRNA gene sequences revealed that all three isolates V-022(T), Ch-022 and Yu-022 exhibited almost identical 16S rRNA gene sequences (99.9% gene sequence similarity) and formed a new lineage within the class Alphaproteobacteria. The name Methylorosula polaris is suggested to accommodate this new genus and novel species with strain V-022(T) (=DSM 22001(T)=VKM V-2485(T)) as the type strain of the type species.
authors.affiliation: S. N. Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospect 60-let Octyabrya 7/2, Moscow 117312, Russia.
authors.forename: Julia Ju
authors.initials: JJ
authors.lastname: Berestovskaya
authors.affiliation: Technical University Braunschweig, Spielmannstrasse 7, Braunschweig, Germany.
authors.forename: Oleg R
authors.initials: OR
authors.lastname: Kotsyurbenko
authors.affiliation: S. N. Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospect 60-let Octyabrya 7/2, Moscow 117312, Russia.
authors.forename: Tatyana P
authors.initials: TP
authors.lastname: Tourova
authors.affiliation: S. N. Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospect 60-let Octyabrya 7/2, Moscow 117312, Russia.
authors.forename: Tatyana V
authors.initials: TV
authors.lastname: Kolganova
authors.affiliation: G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Russia.
authors.forename: Nina V
authors.initials: NV
authors.lastname: Doronina
authors.affiliation: School of Biological Sciences, Bangor University, Bangor LL57 2UW, Gwynedd, Wales, UK.
authors.forename: Peter N
authors.initials: PN
authors.lastname: Golyshin
authors.affiliation: S. N. Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospect 60-let Octyabrya 7/2, Moscow 117312, Russia.
authors.forename: Lina V
authors.initials: LV
authors.lastname: Vasilyeva
mesh_terms: D000332:Aerobiosis; D001482:Base Composition; D042062:Beijerinckiaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D002244:Carbon / Q000378:metabolism*; D016000:Cluster Analysis; D003080:Cold Temperature; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004734:Energy Metabolism; D006863:Hydrogen-Ion Concentration; D008124:Locomotion; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012426:Russia; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D053833:Wetlands
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D002244:Carbon