title: Additive inheritance of histone modifications in Arabidopsis thaliana intra-specific hybrids.
abstract: Plant genomes are earmarked with defined patterns of chromatin marks. Little is known about the stability of these epigenomes when related, but distinct genomes are brought together by intra-species hybridization. Arabidopsis thaliana accessions and their reciprocal hybrids were used as a model system to investigate the dynamics of histone modification patterns. The genome-wide distribution of histone modifications H3K4me2 and H3K27me3 in the inbred parental accessions Col-0, C24 and Cvi and their hybrid offspring was compared by chromatin immunoprecipitation in combination with genome tiling array hybridization. The analysis revealed that, in addition to DNA sequence polymorphisms, chromatin modification variations exist among accessions of A. thaliana. The range of these variations was higher for H3K27me3 (typically a repressive mark) than for H3K4me2 (typically an active mark). H3K4me2 and H3K27me3 were rather stable in response to intra-species hybridization, with mainly additive inheritance in hybrid offspring. In conclusion, intra-species hybridization does not result in gross changes to chromatin modifications.
authors.affiliation: Leibniz Institute of Plant Genetics and Crop Plant Research, Corrensstraße 3, 06466 Gatersleben, Germany.
authors.forename: Ali M Banaei
authors.initials: AM
authors.lastname: Moghaddam
authors.forename: Francois
authors.initials: F
authors.lastname: Roudier
authors.forename: Michael
authors.initials: M
authors.lastname: Seifert
authors.forename: Caroline
authors.initials: C
authors.lastname: Bérard
authors.forename: Marie-Laure M
authors.initials: ML
authors.lastname: Magniette
authors.forename: Raheleh Karimi
authors.initials: RK
authors.lastname: Ashtiyani
authors.forename: Andreas
authors.initials: A
authors.lastname: Houben
authors.forename: Vincent
authors.initials: V
authors.lastname: Colot
authors.forename: Michael F
authors.initials: MF
authors.lastname: Mette
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000378:metabolism; D002843:Chromatin / Q000235:genetics; D055028:Comparative Genomic Hybridization; D004251:DNA Transposable Elements / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D044127:Epigenesis, Genetic; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant / Q000235:genetics*; D011495:Histone-Lysine N-Methyltransferase / Q000378:metabolism*; D006657:Histones / Q000378:metabolism*; D006824:Hybridization, Genetic; D008745:Methylation; D020411:Oligonucleotide Array Sequence Analysis; D011110:Polymorphism, Genetic / Q000235:genetics*; D011499:Protein Processing, Post-Translational
chemical_list: D029681:Arabidopsis Proteins; D002843:Chromatin; D004251:DNA Transposable Elements; D018744:DNA, Plant; D006657:Histones; D011495:Histone-Lysine N-Methyltransferase