title: Effects of HuangKui capsules on glibenclamide pharmacokinetics in rats.
abstract: BACKGROUND
  The main components of HuangKui capsules' are the total flavonoids extracted from the flowers of Abelmoschus manihot L medic. They have been widely used to treat chronic glomerulonephritis, diabetic nephropathy and nephrotic syndrome. The combination of HuangKui capsules and glibenclamide is a possible therapy for patients with diabetes mellitus and diabetic nephropathy. However, there is no report about effects of HuangKui capsules on the glibenclamide pharmacokinetics till now.
  
  
  OBJECTIVE
  This study was aimed investigating the effect of HuangKui capsules on pharmacokinetics of glibenclamide in rats.
  
  
  METHODS
  Eight rats were administered with an oral dose of HuangKui capsules (0.75gkg(-1)) once daily for 10 consecutive days. All the rats were administered orally with the glibenclamide (1mgkg(-1)) before the first time and after the last time given HuangKui capsules. LC-MS/MS was utilized to determine the concentration of glibenclamide in rat plasma and to calculate the corresponding pharmacokinetic parameters. The statistical differences of the two cycles were evaluated by paired-samples t-test.
  
  
  RESULTS
  In the rats treated with HuangKui capsules and glibenclamide, the t(1/2), the time point of maximum plasma concentration (T(max)) of glibenclamide increased obviously (p<0.05) compared with the glibenclamide alone, while maximum plasma concentrations (C(max)), area under the plasma concentration-time curve (AUC((0-t))) decreased significantly (p<0.05). There was no significant difference between other parameters.
  
  
  CONCLUSIONS
  HuangKui capsules can reduce the absorption of glibenclamide and accelerate the metabolism of glibenclamide.
authors.affiliation: Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China.
authors.forename: Zixiu
authors.initials: Z
authors.lastname: Liu
authors.forename: Shijia
authors.initials: S
authors.lastname: Liu
authors.forename: Ling
authors.initials: L
authors.lastname: Zhou
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Gao
authors.forename: Wenzheng
authors.initials: W
authors.lastname: Ju
authors.forename: Hengshan
authors.initials: H
authors.lastname: Tan
authors.forename: Chen
authors.initials: C
authors.lastname: Yang
mesh_terms: D000042:Absorption / Q000187:drug effects; D000818:Animals; D019540:Area Under Curve; D002214:Capsules; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005905:Glyburide / Q000097:blood / Q000493:pharmacokinetics*; D041743:Herb-Drug Interactions; D007004:Hypoglycemic Agents / Q000097:blood / Q000493:pharmacokinetics*; D008297:Male; D019660:Malvaceae; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D002214:Capsules; D004365:Drugs, Chinese Herbal; D007004:Hypoglycemic Agents; D005905:Glyburide