title: Mapping signals that are important for nuclear and nucleolar localization in MCRS2.
abstract: MCRS2 is an oncoprotein that is sequestered in the nucleolus. When in the nucleolus, it promotes the transcription of the rRNA gene. MCRS2 also brings proteins into the nucleolus to change their function. This study analyzes the sequence of MCRS2 and determines that the nuclear localization signal, which has the sequence KRKK, is situated between amino acids 66 and 69. Meanwhile, MCRS2 contains a bipartite nucleolar localization signal, which comprises a KKSK motif, located between amino acids 133 and 136, and a downstream 152-amino acid region, from amino acid 314 to 465. The results of this study are important to understand the function of MCRS2.
authors.affiliation: Department of Microbiology and Immunology, Chang-Gung University, Kwei-Shan, Taoyuan, 33302, Taiwan.
authors.forename: Chung-Wen
authors.initials: CW
authors.lastname: Kuo
authors.forename: Wen-Hung
authors.initials: WH
authors.lastname: Wang
authors.forename: Shih-Tung
authors.initials: ST
authors.lastname: Liu
mesh_terms: D002466:Cell Nucleolus / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D019913:Nuclear Localization Signals / Q000235:genetics / Q000378:metabolism*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D017384:Sequence Deletion
chemical_list: C113292:MCRS1 protein, human; D019913:Nuclear Localization Signals; D009687:Nuclear Proteins; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins