title: Towards establishment of a rice stress response interactome.
abstract: Rice (Oryza sativa) is a staple food for more than half the world and a model for studies of monocotyledonous species, which include cereal crops and candidate bioenergy grasses. A major limitation of crop production is imposed by a suite of abiotic and biotic stresses resulting in 30%-60% yield losses globally each year. To elucidate stress response signaling networks, we constructed an interactome of 100 proteins by yeast two-hybrid (Y2H) assays around key regulators of the rice biotic and abiotic stress responses. We validated the interactome using protein-protein interaction (PPI) assays, co-expression of transcripts, and phenotypic analyses. Using this interactome-guided prediction and phenotype validation, we identified ten novel regulators of stress tolerance, including two from protein classes not previously known to function in stress responses. Several lines of evidence support cross-talk between biotic and abiotic stress responses. The combination of focused interactome and systems analyses described here represents significant progress toward elucidating the molecular basis of traits of agronomic importance.
authors.affiliation: Department of Plant Pathology, University of California Davis, Davis, California, United States of America.
authors.forename: Young-Su
authors.initials: YS
authors.lastname: Seo
authors.forename: Mawsheng
authors.initials: M
authors.lastname: Chern
authors.forename: Laura E
authors.initials: LE
authors.lastname: Bartley
authors.forename: Muho
authors.initials: M
authors.lastname: Han
authors.forename: Ki-Hong
authors.initials: KH
authors.lastname: Jung
authors.forename: Insuk
authors.initials: I
authors.lastname: Lee
authors.forename: Harkamal
authors.initials: H
authors.lastname: Walia
authors.forename: Todd
authors.initials: T
authors.lastname: Richter
authors.forename: Xia
authors.initials: X
authors.lastname: Xu
authors.forename: Peijian
authors.initials: P
authors.lastname: Cao
authors.forename: Wei
authors.initials: W
authors.lastname: Bai
authors.forename: Rajeshwari
authors.initials: R
authors.lastname: Ramanan
authors.forename: Fawn
authors.initials: F
authors.lastname: Amonpant
authors.forename: Loganathan
authors.initials: L
authors.lastname: Arul
authors.forename: Patrick E
authors.initials: PE
authors.lastname: Canlas
authors.forename: Randy
authors.initials: R
authors.lastname: Ruan
authors.forename: Chang-Jin
authors.initials: CJ
authors.lastname: Park
authors.forename: Xuewei
authors.initials: X
authors.lastname: Chen
authors.forename: Sohyun
authors.initials: S
authors.lastname: Hwang
authors.forename: Jong-Seong
authors.initials: JS
authors.lastname: Jeon
authors.forename: Pamela C
authors.initials: PC
authors.lastname: Ronald
mesh_terms: D000222:Adaptation, Physiological; D003001:Cloning, Molecular; D020869:Gene Expression Profiling; D054884:Host-Pathogen Interactions / Q000235:genetics*; D007113:Immunity, Innate; D012275:Oryza / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D010641:Phenotype; D010935:Plant Diseases / Q000276:immunology / Q000517:prevention & control; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D025941:Protein Interaction Mapping; D013312:Stress, Physiological; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D014974:Xanthomonas / Q000472:pathogenicity
chemical_list: D010940:Plant Proteins; D014157:Transcription Factors