title: Lobophorin C and D, new kijanimicin derivatives from a marine sponge-associated actinomycetal strain AZS17.
abstract: Marine sponge Hymeniacidon sp. was collected from coastal waters of the East China Sea to isolate symbiotic microorganisms. The resulting sponge-associated actinomycete, Streptomyces carnosus strain AZS17, was cultivated in a 20 L volume of medium for production of bioactive secondary metabolites. Bioassay-guided isolation and purification by varied chromatographic methods yielded two new compounds of kijanimicin derivatives, AS7-2 and AS9-12. Their structures were elucidated by spectroscopy and comparison with literatures. Results showed these two compounds were structurally similar to the previously reported compounds lobophorin A and B, yet differed in specific bond forms, stereochemistry and optical activities. The two novel compounds were named lobophorin C and D. In vitro cytotoxicity investigation by MTT assay indicated their selective activities. Lobophorin C displayed potent cytotoxic activity against the human liver cancer cell line 7402, while lobophorin D showed significant inhibitory effect on human breast cancer cells MDA-MB 435.
authors.affiliation: College of Marine Science, Zhejiang Ocean University, Zhoushan 316004, China. windswingcn@yahoo.com.cn
authors.forename: Rong-Bian
authors.initials: RB
authors.lastname: Wei
authors.forename: Tao
authors.initials: T
authors.lastname: Xi
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.forename: Fu-Chao
authors.initials: FC
authors.lastname: Li
authors.forename: Yong-Cheng
authors.initials: YC
authors.lastname: Lin
authors.forename: Song
authors.initials: S
authors.lastname: Qin
mesh_terms: D000617:Aminoglycosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology; D001681:Biological Assay; D001943:Breast Neoplasms / Q000188:drug therapy / Q000473:pathology; D045744:Cell Line, Tumor; D002681:China; D005260:Female; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy / Q000473:pathology; D009792:Oceans and Seas; D011161:Porifera / Q000382:microbiology*; D013057:Spectrum Analysis; D013302:Streptomyces / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D000617:Aminoglycosides; D000970:Antineoplastic Agents; C560852:lobophorin C; C560853:lobophorin D