title: Deep sequencing of naupliar-, cyprid- and adult-specific normalised Expressed Sequence Tag (EST) libraries of the acorn barnacle Balanus amphitrite.
abstract: In order to improve the genetic characterisation of the barnacle Balanus amphitrite, normalised EST libraries for the developmental stages, viz. nauplius (a mix of instars I and II), cyprid and adult, were generated. The libraries were sequenced independently using 454 technologies and 575,666 reads were generated. For adults, 4843 unique isotigs were estimated and 6754 and 7506 in the cyprid and naupliar stage, respectively. It was found that some of the previously proposed cyprid-specific bcs genes were also expressed during the naupliar and adult stage. Furthermore, as lectins have been hypothesised to influence settlement cue recognition in barnacles, the database was searched for lectin-like isotigs. Two proteins, uniquely expressed in either the cyprid or the adult stage, matched a mannose receptor, and their nucleotide sequences were 33% and 31% identical to a lectin (BRA-3) isolated from Megabalanus rosa. Further characterisation of these genes may suggest their involvement in settlement.
authors.affiliation: School of Marine Science and Technology, Ridley Building, Newcastle University, UK.
authors.forename: Tristano Bacchetti
authors.initials: TB
authors.lastname: De Gregoris
authors.forename: Oliver
authors.initials: O
authors.lastname: Rupp
authors.forename: Sven
authors.initials: S
authors.lastname: Klages
authors.forename: Florian
authors.initials: F
authors.lastname: Knaust
authors.forename: Thomas
authors.initials: T
authors.lastname: Bekel
authors.forename: Michael
authors.initials: M
authors.lastname: Kube
authors.forename: J Grant
authors.initials: JG
authors.lastname: Burgess
authors.forename: Maria Ina
authors.initials: MI
authors.lastname: Arnone
authors.forename: Alexander
authors.initials: A
authors.lastname: Goesmann
authors.forename: Richard
authors.initials: R
authors.lastname: Reinhardt
authors.forename: Anthony S
authors.initials: AS
authors.lastname: Clare
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D057219:Biofouling; D030541:Databases, Genetic; D020224:Expressed Sequence Tags; D015723:Gene Library; D008018:Life Cycle Stages; D017422:Sequence Analysis, DNA; D001468:Thoracica / Q000235:genetics* / Q000254:growth & development; D014584:User-Computer Interface