title: Expression of human factors CD81, claudin-1, scavenger receptor, and occludin in mouse hepatocytes does not confer susceptibility to HCV entry.
abstract: No suitable mouse model is available for studying chronic liver disease caused by hepatitis C virus (HCV). CD81, claudin-1, scavenger receptor class B type I, and occludin were recently reported to be the important factors in HCV entry into hepatocytes. We made transgenic mice (Alb-CCSO) expressing the four human proteins and examined whether HCV from a patient serum or HCV pseudoparticles (HCVpp) were capable of infecting them. HCV was not detected in the mouse serum after injecting the mice with HCV from a patient serum. We also found no indications of HCVpp entry into primary hepatocytes from Alb-CCSO mice. In addition, HCV-infectible Hep3B cells were fused with HCV-resistant primary mouse hepatocytes and the fused cells showed 35-fold lower infectivity compared to wild-type Hep3B cells, indicating that primary mouse hepatocytes have the inhibitory factor(s) in HCVpp entry. Our results suggest that the expression of the human factors does not confer susceptibility to HCV entry into the liver.
authors.affiliation: Department of Biochemistry, Hamamatsu University School of Medicine, Handa-yama, Higashi-ku, Hamamatsu, Shizuoka, Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Hikosaka
authors.forename: Hidenao
authors.initials: H
authors.lastname: Noritake
authors.forename: Wataru
authors.initials: W
authors.lastname: Kimura
authors.forename: Nishat
authors.initials: N
authors.lastname: Sultana
authors.forename: Mohammad T K
authors.initials: MT
authors.lastname: Sharkar
authors.forename: Yoh-Ichi
authors.initials: Y
authors.lastname: Tagawa
authors.forename: Tadayoshi
authors.initials: T
authors.lastname: Uezato
authors.forename: Yoshimasa
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: Takaji
authors.initials: T
authors.lastname: Wakita
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Miura
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D002459:Cell Fusion; D002478:Cells, Cultured; D062445:Claudin-1; D005260:Female; D015870:Gene Expression; D016174:Hepacivirus / Q000502:physiology*; D006526:Hepatitis C / Q000235:genetics* / Q000378:metabolism / Q000635:transmission / Q000821:virology; D022781:Hepatocytes / Q000378:metabolism / Q000821:virology*; D006801:Humans; D009052:Leukemia Virus, Murine / Q000235:genetics / Q000378:metabolism; D008099:Liver / Q000378:metabolism / Q000821:virology*; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D062793:Occludin; D011991:Receptors, Virus / Q000235:genetics* / Q000378:metabolism; D051122:Scavenger Receptors, Class B / Q000235:genetics / Q000378:metabolism; D060225:Tetraspanin 28; D014771:Virion / Q000235:genetics / Q000378:metabolism; D053586:Virus Internalization
chemical_list: D015703:Antigens, CD; C554798:CD81 protein, human; C568108:CLDN1 protein, human; C554799:Cd81 protein, mouse; D062445:Claudin-1; C568113:Cldn1 protein, mouse; D008565:Membrane Proteins; C568985:OCLN protein, human; D062793:Occludin; C568986:Ocln protein, mouse; D011991:Receptors, Virus; D051122:Scavenger Receptors, Class B; D060225:Tetraspanin 28