title: Influence of retrovirally transduced human tumor-necrosis-factor-alpha on the expression of C-myc, k-ras, C-jun, p53, tgf-alpha, and cea in human colon-carcinoma cell-lines.
abstract: Northern slot blot hybridization and immunohistochemical staining were applied for the characterization of tumor necrosis factor alpha (TNF)-transduced human colon carcinoma cell lines. A significant decrease in the CEA-specific mRNA and protein was observed in TNF-transduced tumor cells LS174T and LoVo while other probes (c-myc, K-ras, c-jun, p53, TGF alpha) as well as anti-K-ras- and anti-p53-antibodies failed to detect differences between cytokine-transduced and parental tumor cells.
authors.forename: W
authors.initials: W
authors.lastname: Uckert
authors.forename: W
authors.initials: W
authors.lastname: Walther
authors.forename: O
authors.initials: O
authors.lastname: Hummel