title: Purification, crystallization and preliminary data analysis of the ligand-receptor complex of the growth and differentiation factor 5 variant R57A (GDF5R57A) and BMP receptor IA (BRIA).
abstract: The binary ligand-receptor complex of human growth and differentiation factor 5 (GDF5) bound to its type I receptor BMP receptor IA (BRIA) was prepared and crystallized. By utilizing the GDF5 variant R57A, which exhibits a high affinity in the subnanomolar range for BRIA, the binary complex of GDF5R57A bound to the extracellular domain of BRIA could be produced and purified. Crystals of this complex belonged to a monoclinic space group: either I2, with unit-cell parameters a = 63.81, b = 62.85, c = 124.99 Å, β = 95.9°, or C2, with unit-cell parameters a = 132.17, b = 62.78, c = 63.53 Å, β = 112.8°.
authors.affiliation: Lehrstuhl für Physiologische Chemie II, Theodor-Boveri-Institut für Biowissenschaften (Biozentrum) der Universität Würzburg, Am Hubland, D-97074 Würzburg, Germany.
authors.forename: Joachim
authors.initials: J
authors.lastname: Nickel
authors.forename: Alexander
authors.initials: A
authors.lastname: Kotzsch
authors.forename: Walter
authors.initials: W
authors.lastname: Sebald
authors.forename: Thomas D
authors.initials: TD
authors.lastname: Mueller
mesh_terms: D052005:Bone Morphogenetic Protein Receptors, Type I / Q000737:chemistry* / Q000302:isolation & purification; D003460:Crystallization; D018360:Crystallography, X-Ray; D055428:Growth Differentiation Factor 5 / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008024:Ligands; D009154:Mutation; D011485:Protein Binding
chemical_list: C525893:GDF5 protein, human; D055428:Growth Differentiation Factor 5; D008024:Ligands; D052005:Bone Morphogenetic Protein Receptors, Type I