title: A potential for granulocyte-colony stimulating factor for use as a prophylactic agent for heatstroke in rats.
abstract: Heatstroke is a form of excessive hyperthermia associated with a systemic inflammatory response that leads to multi-organ dysfunction in which central nervous system disorders predominate. Herein we determined to ascertain whether heat-induced multi-organ dysfunction in rats could be attenuated by granulocyte-colony stimulating factor (G-CSF) preconditioning. Anesthetized rats were divided into 2 major groups and given vehicle solution (isotonic saline, 0.3 ml, subcutaneously) or G-CSF (50-200 μg/kg body weight in 0.3 ml normal saline, subcutaneously) daily and consecutively for 5 days before the start of thermal experiments. They were exposed to an ambient temperature of 43°C for 68 min to induce heatstroke. G-CSF preconditioning significantly prolonged the survival time in heatstroke rats in a dose-related way (82-98 min vs 127-243 min). The non-preconditioning heatstroke animals showed hyperthermia, arterial hypotension, increased serum levels of systemic inflammatory response molecules, increased hypothalamic apoptotic cell numbers as well as neuronal damage scores, and increased serum levels of renal and hepatic dysfunction indicators. These heatstroke syndromes could be significantly reduced by G-CSF preconditioning. Thus our results revealed a potential for G-CSF used as a prophylactic agent for heatstroke in rats.
authors.affiliation: Department of Cardiovascular Surgery, Taiwan Adventist Hospital, School of Medicine, National Yang-Ming University, Taipei, Taiwan.
authors.forename: Ming-Chi
authors.initials: MC
authors.lastname: Yung
authors.forename: Chuan-Chih
authors.initials: CC
authors.lastname: Hsu
authors.forename: Chieh-Yi
authors.initials: CY
authors.lastname: Kang
authors.forename: Chia-Li
authors.initials: CL
authors.lastname: Lin
authors.forename: Shu-Ling
authors.initials: SL
authors.lastname: Chang
authors.forename: Jhi-Joung
authors.initials: JJ
authors.lastname: Wang
authors.forename: Mao-Tsun
authors.initials: MT
authors.lastname: Lin
authors.forename: Pei-Jarn
authors.initials: PJ
authors.lastname: Chen
authors.forename: Sheng-Hsien
authors.initials: SH
authors.lastname: Chen
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001854:Bone Marrow Cells / Q000473:pathology; D002452:Cell Count; D042783:Endothelial Cells / Q000473:pathology; D005334:Fever / Q000150:complications / Q000188:drug therapy; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology* / Q000627:therapeutic use; D018883:Heat Stroke / Q000150:complications / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D022781:Hepatocytes / Q000187:drug effects / Q000473:pathology; D006801:Humans; D007022:Hypotension / Q000150:complications / Q000188:drug therapy; D007031:Hypothalamus / Q000473:pathology; D007249:Inflammation / Q000378:metabolism; D007668:Kidney / Q000187:drug effects / Q000473:pathology; D008297:Male; D009414:Nerve Growth Factors / Q000378:metabolism; D009474:Neurons / Q000187:drug effects / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins; D013234:Stem Cells / Q000187:drug effects / Q000473:pathology; D016019:Survival Analysis; D013997:Time Factors; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D009414:Nerve Growth Factors; D011994:Recombinant Proteins; D042461:Vascular Endothelial Growth Factor A; D016179:Granulocyte Colony-Stimulating Factor