title: Asp2 and Asp3 interact directly with GspB, the export substrate of the Streptococcus gordonii accessory Sec System.
abstract: GspB is a serine-rich glycoprotein adhesin of Streptococcus gordonii that is exported to the bacterial surface by the accessory Sec system. This dedicated export pathway is comprised of seven components (SecA2, SecY2, and five accessory Sec proteins [Asp1 to Asp5]). The latter proteins have no known homologs beyond the Asps of other species. Asp1 to Asp3 are absolutely required for export of the substrate GspB, but their roles in this process are unknown. Using copurification analysis and far-Western blotting, we found that Asp2 and Asp3 could individually bind the serine-rich repeat (SRR) domains of GspB. Deletion of both SRR regions of GspB led to a decrease in its export, suggesting that binding of the Asps to the SRR regions is important for GspB transport by the accessory Sec system. The Asps also bound a heterologous substrate for the accessory Sec system containing a slow-folding MalE variant, but they did not bind wild-type MalE. The combined results indicate that the Asps may recognize the export substrate through preferential interactions with its unstructured or unfolded regions. Glycosylation of the SRR domains on GspB prevented Asp binding, suggesting that binding of the Asps to the preprotein occurs prior to its full glycosylation. Together, these findings suggest that Asp2 and Asp3 are likely to function in part as chaperones in the early phase of GspB transport.
authors.affiliation: San Francisco VA Medical Center, San Francisco, CA 94121, USA.
authors.forename: Yihfen T
authors.initials: YT
authors.lastname: Yen
authors.forename: Ravin
authors.initials: R
authors.lastname: Seepersaud
authors.forename: Barbara A
authors.initials: BA
authors.lastname: Bensing
authors.forename: Paul M
authors.initials: PM
authors.lastname: Sullam
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001665:Binding Sites; D026481:Blotting, Far-Western; D026901:Membrane Transport Proteins / Q000302:isolation & purification / Q000378:metabolism*; D018832:Molecular Chaperones / Q000302:isolation & purification / Q000378:metabolism; D050505:Mutant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D011485:Protein Binding; D025941:Protein Interaction Mapping; D017384:Sequence Deletion; D054773:Streptococcus gordonii / Q000378:metabolism*
chemical_list: D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D026901:Membrane Transport Proteins; D018832:Molecular Chaperones; D050505:Mutant Proteins