title: Hedgehog signaling controls homeostasis of adult intestinal smooth muscle.
abstract: The Hedgehog (Hh) pathway plays multiple patterning roles during development of the mammalian gastrointestinal tract, but its role in adult gut function has not been extensively examined. Here we show that chronic reduction in the combined epithelial Indian (Ihh) and Sonic (Shh) hedgehog signal leads to mislocalization of intestinal subepithelial myofibroblasts, loss of smooth muscle in villus cores and muscularis mucosa as well as crypt hyperplasia. In contrast, chronic over-expression of Ihh in the intestinal epithelium leads to progressive expansion of villus smooth muscle, but does not result in reduced epithelial proliferation. Together, these mouse models show that smooth muscle populations in the adult intestinal lamina propria are highly sensitive to the level of Hh ligand. We demonstrate further that Hh ligand drives smooth muscle differentiation in primary intestinal mesenchyme cultures and that cell-autonomous Hh signal transduction in C3H10T1/2 cells activates the smooth muscle master regulator Myocardin (Myocd) and induces smooth muscle differentiation. The rapid kinetics of Myocd activation by Hh ligands as well as the presence of an unusual concentration of Gli sties in this gene suggest that regulation of Myocd by Hh might be direct. Thus, these data indicate that Hh is a critical regulator of adult intestinal smooth muscle homeostasis and suggest an important link between Hh signaling and Myocd activation. Moreover, the data support the idea that lowered Hh signals promote crypt expansion and increased epithelial cell proliferation, but indicate that chronically increased Hh ligand levels do not dampen crypt proliferation as previously proposed.
authors.affiliation: Department of Cell and Developmental Biology, University of Michigan Medical School, 109 Zina Pitcher Place, Ann Arbor, MI 48109, USA.
authors.forename: William J
authors.initials: WJ
authors.lastname: Zacharias
authors.forename: Blair B
authors.initials: BB
authors.lastname: Madison
authors.forename: Katherine E
authors.initials: KE
authors.lastname: Kretovich
authors.forename: Katherine D
authors.initials: KD
authors.lastname: Walton
authors.forename: Neil
authors.initials: N
authors.lastname: Richards
authors.forename: Aaron M
authors.initials: AM
authors.lastname: Udager
authors.forename: Xing
authors.initials: X
authors.lastname: Li
authors.forename: Deborah L
authors.initials: DL
authors.lastname: Gumucio
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000502:physiology; D053823:Hedgehog Proteins / Q000235:genetics / Q000378:metabolism*; D006706:Homeostasis; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000166:cytology / Q000254:growth & development; D051741:Kruppel-Like Transcription Factors / Q000502:physiology; D008648:Mesoderm; D051379:Mice; D008822:Mice, Transgenic; D009130:Muscle, Smooth / Q000502:physiology*; D058628:Myofibroblasts; D009687:Nuclear Proteins / Q000378:metabolism*; D015398:Signal Transduction; D015534:Trans-Activators / Q000378:metabolism*; D000071676:Zinc Finger Protein GLI1; D000099251:Myocardin
chemical_list: D053823:Hedgehog Proteins; D051741:Kruppel-Like Transcription Factors; D009687:Nuclear Proteins; D015534:Trans-Activators; D000071676:Zinc Finger Protein GLI1; D000099251:Myocardin; C498520:Gli1 protein, mouse