title: A catabolic pathway for the degradation of chrysene by Pseudoxanthomonas sp. PNK-04.
abstract: The chrysene-degrading bacterium Pseudoxanthomonas sp. PNK-04 was isolated from a coal sample. Three novel metabolites, hydroxyphenanthroic acid, 1-hydroxy-2-naphthoic acid and salicylic acid, were identified by TLC, HPLC and MS. Key enzyme activities, namely 1-hydroxy-2-naphthoate hydroxylase, 1,2-dihydroxynaphthalene dioxygenase, salicylaldehyde dehydrogenase and catechol-1,2-dioxygenase, were noted in the cell-free extract. These results suggest that chrysene is catabolized via hydroxyphenanthroic acid, 1-hydroxy-2-naphthoic acid, salicylic acid and catechol. The terminal aromatic metabolite, catechol, is then catabolized by catechol-1,2-dioxygenase to cis,cis-muconic acid, ultimately forming TCA cycle intermediates. Based on these studies, the proposed catabolic pathway for chrysene degradation by strain PNK-04 is chrysene → hydroxyphenanthroic acid → 1-hydroxy-2-naphthoic acid → 1,2-dihydroxynaphthalene → salicylic acid → catechol →cis,cis-muconic acid.
authors.affiliation: Department of Biochemistry, Gulbarga University, Karnataka, India.
authors.forename: Anand S
authors.initials: AS
authors.lastname: Nayak
authors.forename: Sanganal
authors.initials: S
authors.lastname: Sanjeev Kumar
authors.forename: Mudde
authors.initials: M
authors.lastname: Santosh Kumar
authors.forename: Oblesha
authors.initials: O
authors.lastname: Anjaneya
authors.forename: Timmanagouda B
authors.initials: TB
authors.lastname: Karegoudar
mesh_terms: D002264:Carboxylic Acids / Q000378:metabolism; D050548:Catechol 1,2-Dioxygenase / Q000378:metabolism; D002396:Catechols / Q000378:metabolism; D002911:Chrysenes / Q000378:metabolism*; D003031:Coal / Q000382:microbiology; D053858:Metabolic Networks and Pathways; D009281:Naphthalenes / Q000378:metabolism; D020156:Salicylic Acid / Q000378:metabolism; D013011:Sorbic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D044166:Xanthomonadaceae / Q000254:growth & development / Q000378:metabolism*
chemical_list: D002264:Carboxylic Acids; D002396:Catechols; D002911:Chrysenes; D003031:Coal; D009281:Naphthalenes; C031180:chrysene; C085312:1-naphthoic acid; C005939:muconic acid; D050548:Catechol 1,2-Dioxygenase; C034221:catechol; D020156:Salicylic Acid; D013011:Sorbic Acid