title: 1H-Detected 13C photo-CIDNP as a sensitivity enhancement tool in solution NMR.
abstract: NMR is a powerful yet intrinsically insensitive technique. The applicability of NMR to chemical and biological systems would be substantially extended by new approaches going beyond current signal-to-noise capabilities. Here, we exploit the large enhancements arising from (13)C photochemically induced dynamic nuclear polarization ((13)C photo-CIDNP) in solution to improve biomolecular NMR sensitivity in the context of heteronuclear correlation spectroscopy. The (13)C-PRINT pulse sequence presented here involves an initial (13)C nuclear spin polarization via photo-CIDNP followed by conversion to anti-phase coherence and transfer to (1)H for detection. We observe substantial enhancements, up to ≫200-fold, relative to the dark (laser off) experiment. Resonances of both side-chain and backbone CH pairs are enhanced for the three aromatic residues Trp, His, and Tyr, the σ(32) peptide, and the drkN SH3 protein. The sensitivity of this experiment, defined as signal-to-noise per square root of unit time (S/N)(t), is unprecedented in the NMR polarization enhancement literature dealing with polypeptides in solution. Up to a 16-fold larger (S/N)(t) than for the (1)H-(13)C SE-HSQC reference sequence is achieved, for the σ(32) peptide. Data collection time is reduced up to 256-fold, highlighting the advantages of (1)H-detected (13)C photo-CIDNP in solution NMR.
authors.affiliation: Biophysics Graduate Program, University of Wisconsin, Madison, Wisconsin 53706, USA.
authors.forename: Jung Ho
authors.initials: JH
authors.lastname: Lee
authors.forename: Ashok
authors.initials: A
authors.lastname: Sekhar
authors.forename: Silvia
authors.initials: S
authors.lastname: Cavagnero
mesh_terms: D000818:Animals; D002247:Carbon Isotopes; D029721:Drosophila Proteins / Q000737:chemistry; D006360:Heat-Shock Proteins / Q000737:chemistry; D006639:Histidine / Q000737:chemistry; D006859:Hydrogen; D007834:Lasers; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D012808:Sigma Factor / Q000737:chemistry; D012996:Solutions; D014364:Tryptophan / Q000737:chemistry; D014443:Tyrosine / Q000737:chemistry
chemical_list: D002247:Carbon Isotopes; D029721:Drosophila Proteins; D006360:Heat-Shock Proteins; D009587:Nitrogen Isotopes; D012808:Sigma Factor; D012996:Solutions; C080530:drk protein, Drosophila; C069774:heat-shock sigma factor 32; D014443:Tyrosine; D006639:Histidine; D006859:Hydrogen; D014364:Tryptophan