title: Hormonal regulation of galectin 3 in trophoblasts and its effects on endometrium.
abstract: Previous studies had shown important functions of galectin 3 (Gal-3) in endometrium during embryo implantation, in regulation of endometrial cell proliferation and adhesion by interacting with integrin β3. In this study, we investigated hormonal regulation of Gal-3 in trophoblasts and its extracellular effects on endometrium. We used BeWo and RL95-2 cells as a model of trophoblastic and endometrial epithelial cells, respectively, to create an in vivo model of embryo implantation. Our results indicated that 17β-estradiol (E2), progesterone (P4), and human chorionic gonadotropin (hCG) induced the expression of Gal-3 and promoted its secretion from BeWo cells. The exogenous Gal-3 inhibited cell proliferation and induced apoptosis of endometrial cells (RL95-2 cells) through activation of integrin β1. We further validated the proapoptotic effect of Gal-3 secreted by trophoblastic cell on endometrial cells by culturing RL95-2 cells with Bewo cells and measuring the apoptotic rate. Our analysis provides new insight into the critical roles of Gal-3 in embryo implantation.
authors.affiliation: Department of Reproductive Endocrinology, Obstetrics and Gynecology Hospital, Fudan University, Shanghai, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Yang
authors.forename: Hugh S
authors.initials: HS
authors.lastname: Taylor
authors.forename: Caixia
authors.initials: C
authors.lastname: Lei
authors.forename: Chao
authors.initials: C
authors.lastname: Cheng
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D010064:Embryo Implantation / Q000502:physiology; D004717:Endometrium / Q000502:physiology*; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D037502:Galectin 3 / Q000235:genetics* / Q000494:pharmacology / Q000502:physiology; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D019012:Integrin beta1 / Q000502:physiology; D011374:Progesterone / Q000494:pharmacology*; D014327:Trophoblasts / Q000378:metabolism*
chemical_list: D006063:Chorionic Gonadotropin; D037502:Galectin 3; D019012:Integrin beta1; D011374:Progesterone; D004958:Estradiol