title: Sphingobacterium wenxiniae sp. nov., a cypermethrin-degrading species from activated sludge.
abstract: A Gram-negative, non-motile, non-spore-forming, non-flagellated rod capable of degrading cypermethrin, designated LQY-18(T), was isolated from activated sludge of a wastewater treatment plant in China. Strain LQY-18(T) grew at 8-40 °C (optimum 30 °C), at pH 5.0-10.0 (optimum pH 7.0) and with 0-5% (w/v) NaCl (optimum 1%). The predominant menaquinone was MK-7 (97%) and the major fatty acids were summed feature 3 (comprising C(16:1)ω6c and/or C(16:1)ω7c), iso-C(15:0) and iso-C(17:0) 3-OH. The DNA G+C content was 40.3 mol%. Phylogenetic analysis revealed that the isolate belonged to the genus Sphingobacterium of the phylum Bacteroidetes and showed low 16S rRNA gene sequence similarity with recognized members of the genus Sphingobacterium. The closest neighbour was Sphingobacterium mizutaii ATCC 33299(T) (92.9% 16S rRNA gene sequence similarity). On the basis of phenotypic, genetic and phylogenetic data, strain LQY-18(T) (=ACCC 05410(T)=CCTCC AB 2010005(T)=KCTC 23009(T)) should be classified as a representative of a novel species of the genus Sphingobacterium, for which the name Sphingobacterium wenxiniae sp. nov. is proposed.
authors.affiliation: Key Laboratory for Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory for Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
authors.forename: Jin-Wei
authors.initials: JW
authors.lastname: Zheng
authors.affiliation: School of Earth and Environmental Sciences and Research Institute of Oceanography, Seoul National University, Shillim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea.
authors.forename: Byung Cheol
authors.initials: BC
authors.lastname: Cho
authors.affiliation: School of Earth and Environmental Sciences and Research Institute of Oceanography, Seoul National University, Shillim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea.
authors.forename: Chung Yeon
authors.initials: CY
authors.lastname: Hwang
authors.affiliation: College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
authors.forename: Chengxiang
authors.initials: C
authors.lastname: Fang
authors.affiliation: Key Laboratory for Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: He
authors.affiliation: Key Laboratory for Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
authors.forename: Shun-Peng
authors.initials: SP
authors.lastname: Li
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D010802:Phylogeny; D011722:Pyrethrins / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*; D012965:Sodium Chloride / Q000378:metabolism; D045249:Sphingobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D013696:Temperature; D024482:Vitamin K 2 / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D011722:Pyrethrins; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D024482:Vitamin K 2; C017160:cypermethrin; D012965:Sodium Chloride