title: Chemical screening method for the rapid identification of microbial sources of marine invertebrate-associated metabolites.
abstract: Marine invertebrates have proven to be a rich source of secondary metabolites. The growing recognition that marine microorganisms associated with invertebrate hosts are involved in the biosynthesis of secondary metabolites offers new alternatives for the discovery and development of marine natural products. However, the discovery of microorganisms producing secondary metabolites previously attributed to an invertebrate host poses a significant challenge. This study describes an efficient chemical screening method utilizing a 96-well plate-based bacterial cultivation strategy to identify and isolate microbial producers of marine invertebrate-associated metabolites.
authors.affiliation: Department of Chemistry & Department of Biomedical Sciences, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, PEI, Canada. fberrue@upei.ca
authors.forename: Fabrice
authors.initials: F
authors.lastname: Berrue
authors.forename: Sydnor T
authors.initials: ST
authors.lastname: Withers
authors.forename: Brad
authors.initials: B
authors.lastname: Haltli
authors.forename: Jo
authors.initials: J
authors.lastname: Withers
authors.forename: Russell G
authors.initials: RG
authors.lastname: Kerr
mesh_terms: D000818:Animals; D037421:Anthozoa / Q000382:microbiology*; D001419:Bacteria / Q000302:isolation & purification / Q000378:metabolism; D001688:Biological Products / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007448:Invertebrates / Q000382:microbiology*; D008386:Marine Biology; D013058:Mass Spectrometry / Q000379:methods*
chemical_list: D001688:Biological Products