title: D-Ornithine coopts pyrrolysine biosynthesis to make and insert pyrroline-carboxy-lysine.
abstract: D-ornithine has previously been suggested to enhance the expression of pyrrolysine-containing proteins. We unexpectedly discovered that uptake of D-ornithine results in the insertion of a new amino acid, pyrroline-carboxy-lysine (Pcl) instead of the anticipated pyrrolysine (Pyl). Our feeding and biochemical studies point to specific roles of the poorly understood Pyl biosynthetic enzymes PylC and PylD in converting L-lysine and D-ornithine to Pcl and confirm intermediates in the biosynthesis of Pyl.
authors.affiliation: Genomics Institute of the Novartis Research Foundation, San Diego, California, USA.
authors.forename: Susan E
authors.initials: SE
authors.lastname: Cellitti
authors.forename: Weijia
authors.initials: W
authors.lastname: Ou
authors.forename: Hsien-Po
authors.initials: HP
authors.lastname: Chiu
authors.forename: Jan
authors.initials: J
authors.lastname: Grünewald
authors.forename: David H
authors.initials: DH
authors.lastname: Jones
authors.forename: Xueshi
authors.initials: X
authors.lastname: Hao
authors.forename: Qian
authors.initials: Q
authors.lastname: Fan
authors.forename: Lisa L
authors.initials: LL
authors.lastname: Quinn
authors.forename: Kenneth
authors.initials: K
authors.lastname: Ng
authors.forename: Andrew T
authors.initials: AT
authors.lastname: Anfora
authors.forename: Scott A
authors.initials: SA
authors.lastname: Lesley
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Uno
authors.forename: Ansgar
authors.initials: A
authors.lastname: Brock
authors.forename: Bernhard H
authors.initials: BH
authors.lastname: Geierstanger
mesh_terms: D000595:Amino Acid Sequence; D004926:Escherichia coli; D005786:Gene Expression Regulation / Q000502:physiology; D057809:HEK293 Cells; D006801:Humans; D008239:Lysine / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000737:chemistry; D017020:Methanosarcina / Q000235:genetics / Q000378:metabolism; D015394:Molecular Structure; D009952:Ornithine / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D010957:Plasmids; D011401:Promoter Regions, Genetic
chemical_list: C561311:2-amino-6-(3,4-dihydro-2H-pyrrole-2-carboxamido)hexanoic acid; D009952:Ornithine; C456839:pyrrolysine; D008239:Lysine