title: Inactivation of the DNA repair genes mutS, mutL or the anti-recombination gene mutS2 leads to activation of vitamin B1 biosynthesis genes.
abstract: Oxidative stress generates harmful reactive oxygen species (ROS) that attack biomolecules including DNA. In living cells, there are several mechanisms for detoxifying ROS and repairing oxidatively-damaged DNA. In this study, transcriptomic analyses clarified that disruption of DNA repair genes mutS and mutL, or the anti-recombination gene mutS2, in Thermus thermophilus HB8, induces the biosynthesis pathway for vitamin B(1), which can serve as an ROS scavenger. In addition, disruption of mutS, mutL, or mutS2 resulted in an increased rate of oxidative stress-induced mutagenesis. Co-immunoprecipitation and pull-down experiments revealed previously-unknown interactions of MutS2 with MutS and MutL, indicating that these proteins cooperatively participate in the repair of oxidatively damaged DNA. These results suggested that bacterial cells sense the accumulation of oxidative DNA damage or absence of DNA repair activity, and signal the information to the transcriptional regulation machinery for an ROS-detoxifying system.
authors.affiliation: RIKEN SPring-8 Center, Harima Institute, Kouto, Sayo-gun, Hyogo, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Fukui
authors.forename: Taisuke
authors.initials: T
authors.lastname: Wakamatsu
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Agari
authors.forename: Ryoji
authors.initials: R
authors.lastname: Masui
authors.forename: Seiki
authors.initials: S
authors.lastname: Kuramitsu
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D004260:DNA Repair / Q000235:genetics*; D016166:Free Radical Scavengers / Q000378:metabolism; D020868:Gene Silencing; D047468:Immunoprecipitation; D016296:Mutagenesis; D009154:Mutation; D018384:Oxidative Stress / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism; D011995:Recombination, Genetic / Q000235:genetics*; D016963:Thermus thermophilus / Q000235:genetics* / Q000378:metabolism; D013831:Thiamine / Q000096:biosynthesis*; D015533:Transcriptional Activation
chemical_list: D001426:Bacterial Proteins; D016166:Free Radical Scavengers; D017382:Reactive Oxygen Species; D013831:Thiamine