title: miR-29c induces cell cycle arrest in esophageal squamous cell carcinoma by modulating cyclin E expression.
abstract: Cyclin E is reported to be an important cell cycle regulator, and its dysregulation is implicated in tumorigenesis including esophageal squamous cell carcinoma (ESCC). MicroRNAs (miRNAs) regulate gene expression at the posttranscriptional level and play important roles in tumor initiation and progression. However, the regulation of cyclin E by miRNAs is still unclear in ESCC. In the present study, we found that overexpression of miR-29c inhibited cyclin E expression by targeting 3' untranslated region of cyclin E messenger RNA in ESCC cells. Moreover, overexpression of miR-29c induced cell cycle G(1)/G(0) arrest through suppression of cyclin E expression, without affecting other G(1) phase-related proteins level, such as cyclin D1, cyclin D2, cyclin dependent kinase (CDK) 2 and CDK6. Furthermore, we demonstrated that overexpression of miR-29c inhibited proliferation of ESCC cells in vitro and in vivo. In addition, we detected miR-29c expression in 26 pairs of esophageal tumor-in-site-tissues and 60 pairs of ESCC tissues. The result showed that miR-29c level significantly decreased in ESCC tumor tissues and cell lines compared with normal esophageal epithelia. Taken together, our findings indicated that miR-29c was frequently downregulated in ESCC tissues and cells and suppressed tumor growth by inducing cell cycle G(1)/G(0) arrest mainly through modulating cyclin E expression.
authors.affiliation: Department of Thoracic Surgery, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 17, Panjiayuannanli, Chaoyang District, Beijing 100021, China.
authors.forename: Da-Peng
authors.initials: DP
authors.lastname: Ding
authors.forename: Zhao-Li
authors.initials: ZL
authors.lastname: Chen
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Zhao
authors.forename: Ji-Wen
authors.initials: JW
authors.lastname: Wang
authors.forename: Jian
authors.initials: J
authors.lastname: Sun
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wang
authors.forename: Feng-Wei
authors.initials: FW
authors.lastname: Tan
authors.forename: Xiao-Gang
authors.initials: XG
authors.lastname: Tan
authors.forename: Bao-Zhong
authors.initials: BZ
authors.lastname: Li
authors.forename: Fang
authors.initials: F
authors.lastname: Zhou
authors.forename: Kang
authors.initials: K
authors.lastname: Shao
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.forename: Bin
authors.initials: B
authors.lastname: Qiu
authors.forename: Jie
authors.initials: J
authors.lastname: He
mesh_terms: D000818:Animals; D001483:Base Sequence; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology*; D002453:Cell Cycle / Q000502:physiology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D019927:Cyclin E / Q000378:metabolism*; D017931:DNA Primers; D004938:Esophageal Neoplasms / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D017403:In Situ Hybridization; D035683:MicroRNAs / Q000502:physiology*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D019927:Cyclin E; D017931:DNA Primers; C532045:MIRN29a microRNA, human; D035683:MicroRNAs