title: Human papillomavirus type-16 (HPV-16) major transforming proteins functionally interact with interferon signaling mechanisms.
abstract: Since the IFN system has been implicated in cell growth and differentiation control mechanisms, we evaluated the influence of the expression of HPV-16 E6 and E7 oncoproteins on IFN signaling by using cotransfection experiments. Both viral oncoproteins differentially interfered with the inducibility of IFN-beta promoter by Sendai virus. The activation by IFN-gamma of a GBP ISRE reporter was dramatically affected by both viral proteins suggesting a disruption of STATs/IRFs function. Further, the inducibility of 6-16 gene ISRE reporter by IFN-alpha was decreased to varying degrees by both viral oncoproteins, implying that ISGF3 function is also impaired. Taken together, these observations suggest that HPV-16 negatively interacts with cellular targets of the IFN system, and these interactions may be implicated in cellular transformation caused by HPVs and their refractory response to IFN treatment.
authors.affiliation: UNIV VIENNA,DEPT CELL & MICROBIOL,INST MICROBIOL & GENET,A-1030 VIENNA,AUSTRIA.
authors.forename: S
authors.initials: S
authors.lastname: Perea
authors.forename: O
authors.initials: O
authors.lastname: Lopezocejo
authors.forename: A
authors.initials: A
authors.lastname: Vongabain
authors.forename: M
authors.initials: M
authors.lastname: Arana