title: Secretion of biologically-active human interferon-β by Bacillus subtilis.
abstract: Human interferon-β (hIFN-β) was used as a heterologous model protein to investigate the effects of the Bacillus subtilis AmyE propeptide and co-expression of PrsA in enhancing the secretion of heterologous proteins in B. subtilis. Secretion and activity of hIFN-β with AmyE propeptide increased by more than four-fold compared to that without AmyE propeptide. Moreover, under conditions of co-expressed PrsA, the secretion production and activity of hIFN-β with AmyE propeptide increased by more than 1.5-fold. AmyE propeptide and co-expression of PrsA thus have an additive effect on enhancing the production of the hIFN-β in B. subtilis.
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki 305-8572, Japan. hkakeshi@sakura.cc.tsukuba.ac.jp
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kakeshita
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Kageyama
authors.forename: Keiji
authors.initials: K
authors.lastname: Endo
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Tohata
authors.forename: Katsutoshi
authors.initials: K
authors.lastname: Ara
authors.forename: Katsuya
authors.initials: K
authors.lastname: Ozaki
authors.forename: Kouji
authors.initials: K
authors.lastname: Nakamura
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001709:Biotechnology / Q000379:methods; D015870:Gene Expression; D006801:Humans; D016899:Interferon-beta / Q000235:genetics / Q000378:metabolism*; D008074:Lipoproteins / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D008074:Lipoproteins; D008565:Membrane Proteins; D011498:Protein Precursors; D011994:Recombinant Proteins; C082160:prsA protein, bacteria; D016899:Interferon-beta