title: Tunable supramolecular hydrogel for in situ encapsulation and sustained release of bioactive lysozyme.
abstract: To develop new matrices for the entrapment and sustained release of bioactive lysozyme, a series of supramolecular hydrogels based on α-cyclodextrin (α-CD) and water-soluble poly(ε-caprolactone)-poly(ethylene glycol) block copolymer (PCL-b-PEG) were prepared in the presence of chicken egg lysozyme. Different from commonly used polymeric microspheres and chemically crosslinked hydrogels for lysozyme encapsulation, such hydrogel matrices could be formed under mild conditions without high temperature and the use of chemical emulsifiers or crosslinkers. Their gelation rate, mechanical strength and shear viscosity as well as the release behavior for the encapsulated lysozyme could be tuned easily by the change of α-CD or PCL-b-PEG amount. For the encapsulated lysozyme, its conformation and biological activity could be well maintained when compared to native lysozyme. For the resultant supramolecular hydrogels, they were also confirmed to have a good biocompatibility by MTT assay using mice skin fibroblast (L929).
authors.affiliation: DSAPM Lab and PCFM Lab, Institute of Polymer Science, School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Ma
authors.forename: Li-Ming
authors.initials: LM
authors.lastname: Zhang
authors.forename: Xi
authors.initials: X
authors.lastname: Xie
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
authors.forename: Min-Qiang
authors.initials: MQ
authors.lastname: Xie
mesh_terms: D000818:Animals; D002460:Cell Line; D002645:Chickens; D003692:Delayed-Action Preparations / Q000737:chemistry*; D005026:Ethylene Glycols / Q000737:chemistry; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D051379:Mice; D009113:Muramidase / Q000008:administration & dosage*; D011091:Polyesters / Q000737:chemistry; D014783:Viscosity; D047391:alpha-Cyclodextrins / Q000737:chemistry
chemical_list: D003692:Delayed-Action Preparations; D005026:Ethylene Glycols; D011091:Polyesters; D047391:alpha-Cyclodextrins; C476300:poly(epsilon-caprolactone)-b-poly(ethylene glycol); D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D009113:Muramidase; C032613:alpha-cyclodextrin