title: Rutin inhibits oleic acid induced lipid accumulation via reducing lipogenesis and oxidative stress in hepatocarcinoma cells.
abstract: Excessive lipid accumulation within liver has been proposed to cause obesity, hyperlipidemia, diabetes, and fatty liver disease. Rutin, a common dietary flavonoid that is consumed in fruits, vegetables, and plant-derived beverages, has various biological functions, including antioxidant, anti-inflammatory, and anticancer effects. However, a hypolipidemic effect of rutin on fatty liver disease has not been reported. In this study, we examined the effect of rutin on reducing lipid accumulation in hepatic cells. Hepatocytes were treated with oleic acid (OA) containing with or without rutin to observe the lipid accumulation by Nile red stain. The result showed rutin suppressed OA-induced lipid accumulation and increased adenosine 5'-monophosphate (AMP)-activated protein kinase (AMPK) activity in hepatocytes. The expression of critical molecule involved in lipid synthesis, sterol regulatory element binding proteins-1 (SREBP-1), was attenuated in rutin-treated cells. Moreover, long-term incubation of rutin inhibited the transcriptions of 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase (HMGCR), glycerol-3-phosphate acyltransferase (GPAT), fatty acid synthase (FAS), and acetyl-coenzyme carboxylase (ACC). Besides, we also found out the antioxidative effect of rutin by increasing the expression of peroxisome proliferator-activated receptor (PPAR)-α and antioxidative enzymes. Taken together, our findings suggest rutin could attenuate lipid accumulation by decreasing lipogenesis and oxidative stress in hepatocyte.
authors.affiliation: Inst of Biochemistry and Biotechnology, Chung Shan Medical Univ, No 110, Section 1, Chien-kauo N Rd Taichung, Taiwan, ROC.
authors.forename: Cheng-Hsun
authors.initials: CH
authors.lastname: Wu
authors.forename: Ming-Cheng
authors.initials: MC
authors.lastname: Lin
authors.forename: Hsueh-Chun
authors.initials: HC
authors.lastname: Wang
authors.forename: Mon-Yuan
authors.initials: MY
authors.lastname: Yang
authors.forename: Ming-Jia
authors.initials: MJ
authors.lastname: Jou
authors.forename: Chau-Jong
authors.initials: CJ
authors.lastname: Wang
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism; D000214:Acyl Coenzyme A / Q000378:metabolism; D015153:Blotting, Western; D064429:Fatty Acid Synthases / Q000378:metabolism; D015870:Gene Expression; D005992:Glycerol-3-Phosphate O-Acyltransferase / Q000378:metabolism; D056945:Hep G2 Cells; D022781:Hepatocytes / Q000378:metabolism; D006801:Humans; D000960:Hypolipidemic Agents / Q000494:pharmacology*; D008055:Lipids / Q000096:biosynthesis; D050155:Lipogenesis / Q000187:drug effects*; D008099:Liver / Q000166:cytology / Q000378:metabolism; D019301:Oleic Acid / Q000378:metabolism*; D010078:Oxazines; D018384:Oxidative Stress / Q000187:drug effects*; D047492:Peroxisome Proliferator-Activated Receptors; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012431:Rutin / Q000494:pharmacology*; D051780:Sterol Regulatory Element Binding Protein 1 / Q000378:metabolism
chemical_list: D000214:Acyl Coenzyme A; D000960:Hypolipidemic Agents; D008055:Lipids; D010078:Oxazines; D047492:Peroxisome Proliferator-Activated Receptors; D051780:Sterol Regulatory Element Binding Protein 1; C008047:3-hydroxy-3-methylglutaryl-coenzyme A; D019301:Oleic Acid; D012431:Rutin; D005992:Glycerol-3-Phosphate O-Acyltransferase; D064429:Fatty Acid Synthases; D055372:AMP-Activated Protein Kinases; C044808:nile red