title: Removal of 3'untranslated sequences dramatically enhances transient expression of ovine follicle-stimulating hormone Beta gene messenger ribonucleic Acid.
abstract: The influence of the 3'untranslated (3'-UT) region of the ovine follicle-stimulating hormone (FSH) β mRNA on the level of transcript expression was studied. Only very low levels of FSH β mRNA were detected following transient transfection of COS cells with a eukaryotic expression vector containing a full-length ovine FSH β cDNA that includes 1.1 kilobases (kb) of 3'-UT. In contrast, deletion of all but 135 basepairs (bp) of the 3'-UT resulted in a striking increase in FSH β mRNA expression following transfection of the truncated cDNA construct. These observations suggest that sequences within the 3'-UT of the ovine FSH β mRNA may play a significant role in the post-transcriptional regulation of FSH β expression.
authors.affiliation: Centre for Animal Biotechnology, School of Veterinary Science, The University of Melbourne, Parkville Victoria 3052, Australia.
authors.forename: P S
authors.initials: PS
authors.lastname: Mountford
authors.forename: M R
authors.initials: MR
authors.lastname: Brandon
authors.forename: T E
authors.initials: TE
authors.lastname: Adams