title: Oceanicoccus sagamiensis gen. nov., sp. nov., a gammaproteobacterium isolated from sea water of Sagami Bay in Japan.
abstract: A gram-negative, motile, coccoid- and amorphous-shaped, non-pigmented chemoheterotrophic bacterium, designated strain PZ-5(T), was isolated from sea water of Sagami Bay in Japan and subjected to a polyphasic taxonomic study. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the novel isolate could be affiliated with the class Gammaproteobacteria. Strain PZ-5(T) showed below 93.9% similarity with validly published bacteria and demonstrated the highest sequence similarity to Dasania marina KOPRI 20902(T) (93.9%). Strain PZ-5(T) formed a monophyletic group with D. marina KOPRI 20902(T). The DNA G+C content of strain PZ-5(T) was 49.8 mol%. The major isoprenoid quinone was Q-8 and predominant cellular fatty acids were C(15:0) ISO 20H (19%), C(16:1) ω7c (17.4%), C(17;1) ω8c (16.2%), C(11:0) 3OH (7.5%), and C(15:1) ω8c (6.5%). Based on evidence from a polyphasic taxonomical study, it was concluded that the strain should be classified as representing a new genus and species of the class Gammaproteobacteria, for which the name Oceanicoccus sagamiensis gen. nov., sp. nov., (type strain PZ-5(T) =NBRC 107125(T) =KCTC 23278(T)) is proposed.
authors.affiliation: Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo 113-0032, Japan. psh214@hotmail.com
authors.forename: Sanghwa
authors.initials: S
authors.lastname: Park
authors.forename: Susumu
authors.initials: S
authors.lastname: Yoshizawa
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Kogure
authors.forename: Akira
authors.initials: A
authors.lastname: Yokota
mesh_terms: D001482:Base Composition; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D020563:Gammaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D007564:Japan; D008124:Locomotion; D008969:Molecular Sequence Data; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D011809:Quinones; D012336:RNA, Ribosomal, 16S