title: Metabolites of arsenic and increased DNA damage of p53 gene in arsenic plant workers.
abstract: Recent studies have shown that monomethylarsonous acid is more cytotoxic and genotoxic than arsenate and arsenite, which may attribute to the increased levels of reactive oxygen species. In this study, we used hydride generation-atomic absorption spectrometry to determine three arsenic species in urine of workers who had been working in arsenic plants,and calculated primary and secondary methylation indexes. The damages of exon 5, 6, 8 of p53 gene were determined by the method developed by Sikorsky, et al. Results show that the concentrations of each urinary arsenic species,and damage indexes of exon 5 and 8 of p53 gene in the exposed population were significantly higher, but SMI was significantly lower than in the control group. The closely positive correlation between the damage index of exon 5 and PMI,MMA, DMA were found, but there was closely negative correlation between the damage index of exon 5 and SMI. Those findings suggested that DNA damage of exon 5 and 8 of p53 gene existed in the population occupationally exposed to arsenic. For exon 5, the important factors may include the model of arsenic metabolic transformation, the concentrations of MMA and DMA, and the MMA may be of great importance.
authors.affiliation: Department of Occupational Health, Yunnan Provincial Center for Disease Control and Prevention, No. 158 Dongsi Street, Kunming, Yunnan, People's Republic of China. Dongsijiehua@sina.com
authors.forename: Weihua
authors.initials: W
authors.lastname: Wen
authors.forename: Jinghua
authors.initials: J
authors.lastname: Wen
authors.forename: Lin
authors.initials: L
authors.lastname: Lu
authors.forename: Hua
authors.initials: H
authors.lastname: Liu
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.forename: Huirong
authors.initials: H
authors.lastname: Cheng
authors.forename: Wangjun
authors.initials: W
authors.lastname: Che
authors.forename: Liang
authors.initials: L
authors.lastname: Li
authors.forename: Guanbei
authors.initials: G
authors.lastname: Zhang
mesh_terms: D001151:Arsenic / Q000378:metabolism / Q000633:toxicity*; D001152:Arsenicals / Q000009:adverse effects / Q000378:metabolism; D002101:Cacodylic Acid / Q000378:metabolism / Q000633:toxicity; D002617:Chemical Industry; D003404:Creatinine / Q000652:urine; D004249:DNA Damage / Q000187:drug effects*; D005091:Exons / Q000187:drug effects; D016158:Genes, p53 / Q000187:drug effects*; D006801:Humans; D016273:Occupational Exposure / Q000009:adverse effects*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D001152:Arsenicals; D002101:Cacodylic Acid; D003404:Creatinine; C020300:monomethylarsonic acid; D001151:Arsenic