title: AEG -1 overexpression: a novel indicator for peritoneal dissemination and lymph node metastasis in epithelial ovarian cancers.
abstract: OBJECTIVE
  Despite advances in chemotherapy and cytoreductive surgery, ovarian cancer remains the most lethal gynecological malignancy with a 5-year survival rate of 25% to 30% in advanced stage disease. Our purpose is to evaluate whether astrocyte elevated gene-1 (AEG-1) is a novel predictor of peritoneal dissemination and lymph node metastasis in epithelial ovarian cancer (EOC), which was not previously studied by others.
  
  
  METHODS
  Through immunohistochemical and Western blot analysis, AEG-1 expression was evaluated in 25 normal ovarian and 157 EOC specimens. The relationship between AEG-1 expression and EOC metastasis was determined by univariate and multivariate analyses.
  
  
  RESULTS
  Western blotting analysis revealed that AEG-1 was overexpressed in metastatic tissues from patients with ovarian cancers. Immunohistochemistry results showed that 83 (95.4%) presented peritoneal dissemination; 41 (47.1%) had lymph node metastasis among 87 patients with AEG-1 overexpression. Univariate and multivariate logistic regression analyses demonstrated that AEG-1 overexpression correlated with peritoneal dissemination and lymph node metastasis in EOC. We further found that the positive and specificity predictive value of AEG-1 staining were better for peritoneal metastasis, whereas the negative and sensitivity predictive value of AEG-1 staining were better for lymph node metastasis. The odds ratio of high-to-low expression for peritoneal dissemination was 8.541 (95% confidence interval, 2.561-37.461), and that for lymph node metastasis was 9.581 (95% confidence interval, 2.613-23.214).
  
  
  CONCLUSIONS
  The present findings indicate that AEG-1 is overexpressed in a great portion of EOC patients with peritoneal dissemination and/or lymph node metastasis and may be clinically useful for predicting metastasis in EOC. Our findings might provide some benefits for metastatic EOC patients in the clinic.
authors.affiliation: Department of Pathology, The Third Affiliated (Tumor) Hospital, Harbin, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Li
authors.forename: Junjun
authors.initials: J
authors.lastname: Liu
authors.forename: Renbo
authors.initials: R
authors.lastname: Lu
authors.forename: Ge
authors.initials: G
authors.lastname: Yu
authors.forename: Xiaochuan
authors.initials: X
authors.lastname: Wang
authors.forename: Yulan
authors.initials: Y
authors.lastname: Zhao
authors.forename: Hongtao
authors.initials: H
authors.lastname: Song
authors.forename: Ping
authors.initials: P
authors.lastname: Lin
authors.forename: Xicai
authors.initials: X
authors.lastname: Sun
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Yu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Ning
authors.forename: Jingshu
authors.initials: J
authors.lastname: Geng
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics; D000077216:Carcinoma, Ovarian Epithelial; D015815:Cell Adhesion Molecules / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008198:Lymph Nodes / Q000473:pathology; D008207:Lymphatic Metastasis; D008565:Membrane Proteins; D008875:Middle Aged; D009375:Neoplasms, Glandular and Epithelial / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology*; D010051:Ovarian Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology*; D010534:Peritoneal Neoplasms / Q000175:diagnosis / Q000235:genetics / Q000556:secondary*; D011379:Prognosis; D016601:RNA-Binding Proteins; D015854:Up-Regulation / Q000235:genetics
chemical_list: D014408:Biomarkers, Tumor; D015815:Cell Adhesion Molecules; C483039:MTDH protein, human; D008565:Membrane Proteins; D016601:RNA-Binding Proteins