title: In vitro study of antibacterial activity of the alga Sargassum oligocystum from the Persian Gulf.
abstract: OBJECTIVE
  With due attention to the development of drug-resistant bacteria, discovering of new antibacterial compounds is needed. Algae produce numerous bioactive substances which may have pharmacological properties such as antibacterial activity. The objective of this investigation was to in vitro study of antibacterial activity of brown alga Sargassum oligocystum collected along the Bushehr coast of Persian Gulf (south west of Iran).
  
  
  METHODS
  Hot water extract, cold water extract, and hot glycerin extract were prepared. The effect of the extracts were investigated on Staphylococcus aureus (ATCC 25923), Staphylococcus epidermidis (ATCC 14990), Pseudomonas aeruginosa (ATCC 27853), and Escherichia coli (ATCC 25922).
  
  
  RESULTS
  Hot water extract exhibited antibacterial activity against Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa. Cold water extract and hot glycerin extract did not show antibacterial activity on any of the four test bacteria. The minimum inhibitory concentration (MIC) of hot water extract for both Staphylococcus aureus and Staphylococcus epidermidis was 3.175 mg/ml. However, the MIC of this extract for Pseudomonas aeruginosa was 9.556 mg/ml.
  
  
  CONCLUSIONS
  In this study gram-positive bacteria were more susceptible to hot water extract than gram-negative bacteria. Extract of Sargassum oligocystum could be a candidate for purification and further in vivo studies.
authors.affiliation: Department of Microbiology and Parasitology, Faculty of Medicine, The Persian Gulf Tropical and Infectious Diseases Research Center, Bushehr University of Medical Sciences, Bushehr, Iran. tajbakhshsaeed@yahoo.com
authors.forename: S
authors.initials: S
authors.lastname: Tajbakhsh
authors.forename: M
authors.initials: M
authors.lastname: Pouyan
authors.forename: K
authors.initials: K
authors.lastname: Zandi
authors.forename: P
authors.initials: P
authors.lastname: Bahramian
authors.forename: K
authors.initials: K
authors.lastname: Sartavi
authors.forename: M
authors.initials: M
authors.lastname: Fouladvand
authors.forename: G
authors.initials: G
authors.lastname: Asayesh
authors.forename: A
authors.initials: A
authors.lastname: Barazesh
mesh_terms: D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D019156:Indian Ocean; D008826:Microbial Sensitivity Tests; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000254:growth & development; D044647:Sargassum / Q000737:chemistry*; D012623:Seawater; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development; D013212:Staphylococcus epidermidis / Q000187:drug effects / Q000254:growth & development
chemical_list: D000900:Anti-Bacterial Agents