title: Formalin-inactivated vaccine provokes cross-protective immunity in a mouse model of human enterovirus 71 infection.
abstract: Human enterovirus 71 (HEV71) has emerged as a major cause of epidemics of hand, foot and mouth disease associated with severe neurological sequelae in the Asia-Pacific region. In this study, a passive protection mouse model was used to evaluate the protective efficacy of formalin-inactivated HEV71 vaccines derived from a Chinese C4 genotype strain. Pregnant mice were immunised using a prime/boost strategy and ≥50U of vaccine protected five-day-old pups from lethal challenge with a mouse-adapted (B3 genotype) strain of HEV71. Immunised mice developed a neutralising antibody response to both the immunising C4 strain and to the mouse-adapted strain. Mice born to immunised dams showed significantly less myositis and reduced viral loads in tissues.
authors.affiliation: Infectious Diseases and Immunology, The University of Sydney, Australia. emily.bek@sydney.edu.au
authors.forename: Emily Jane
authors.initials: EJ
authors.lastname: Bek
authors.forename: Khairunnisa Mohamed
authors.initials: KM
authors.lastname: Hussain
authors.forename: Patchara
authors.initials: P
authors.lastname: Phuektes
authors.forename: Chee Choy
authors.initials: CC
authors.lastname: Kok
authors.forename: Qiang
authors.initials: Q
authors.lastname: Gao
authors.forename: Fang
authors.initials: F
authors.lastname: Cai
authors.forename: Zhenglun
authors.initials: Z
authors.lastname: Gao
authors.forename: Peter Charles
authors.initials: PC
authors.lastname: McMinn
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D056738:Cross Protection; D004195:Disease Models, Animal; D029821:Enterovirus A, Human / Q000276:immunology*; D004769:Enterovirus Infections / Q000517:prevention & control*; D005260:Female; D005557:Formaldehyde; D007117:Immunization, Secondary / Q000379:methods; D051379:Mice; D008807:Mice, Inbred BALB C; D011247:Pregnancy; D012376:Rodent Diseases / Q000517:prevention & control / Q000821:virology; D016019:Survival Analysis; D014611:Vaccination / Q000379:methods; D015164:Vaccines, Inactivated / Q000008:administration & dosage / Q000276:immunology; D019562:Viral Load; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D015164:Vaccines, Inactivated; D014765:Viral Vaccines; D005557:Formaldehyde