title: Decreased expression of p16 gene in gastric cancer.
abstract: We analyzed gastric cancers for p16 gene alterations. Homozygous deletion of the p16 gene was found in 1 of 8 gastric cancer cell lines and none of 20 primary gastric cancers. Mutations of the p16 gene were not detected in exon 1-3. Reverse transcription-polymerase chain reaction (RT-PCR) analysis showed decreased or no expression of the p16 gene in 1 of 8 cell lines (12.5%) and in 8 of 20 tumors (40%), although no homozygous deletions or mutations within exon 1-3 were detected. These results suggest that the p16 gene may correlate to tumorigenesis due to decrease or loss of gene products in gastric cancers.
authors.affiliation: TUFTS UNIV,SCH MED,DEPT BIOCHEM,BOSTON,MA 02111.
authors.forename: S
authors.initials: S
authors.lastname: Fushida
authors.forename: Y
authors.initials: Y
authors.lastname: Yonemura
authors.forename: H
authors.initials: H
authors.lastname: Takamura
authors.forename: Y
authors.initials: Y
authors.lastname: Hirono
authors.forename: I
authors.initials: I
authors.lastname: Miyazaki
authors.forename: T
authors.initials: T
authors.lastname: Urano