title: Identification of proteins differentially expressed in gastric cancer cells with high metastatic potential for invasion to lymph nodes.
abstract: In a search for proteins involved in cancer metastasis, we analyzed proteomes of the human gastric cancer cell OCUM-2M and its metastatic subline OCUM-2MLN. We observed that aspartate aminotransferase (AAT), D-site binding protein (DBP), and anterior gradient protein 2 (AGR2) are differentially expressed in metastatic OCUM-2MLN cells. Measurement of protein expression in clinical samples indicated that DBP and AAT are also down-regulated in metastatic adenocarcinoma. Additionally, urokinase-type tissue plasminogen activator is up-regulated in OCUM-2MLN cells and also in metastatic gastric cancer samples. Collectively, these results raise a possibility that AAT, DBP and AGR2 are functionally implicated in the invasiveness of gastric cancer cells.
authors.affiliation: Department of Biotechnology, Seoul Women's University, Seoul, 139-774, Korea.
authors.forename: Do Hee
authors.initials: DH
authors.lastname: Lee
authors.forename: Youra
authors.initials: Y
authors.lastname: Lee
authors.forename: Joohyun
authors.initials: J
authors.lastname: Ryu
authors.forename: Sung Goo
authors.initials: SG
authors.lastname: Park
authors.forename: Sayeon
authors.initials: S
authors.lastname: Cho
authors.forename: Je-Jung
authors.initials: JJ
authors.lastname: Lee
authors.forename: Chan
authors.initials: C
authors.lastname: Choi
authors.forename: Byoung Chul
authors.initials: BC
authors.lastname: Park
mesh_terms: D002293:Adenocarcinoma, Scirrhous / Q000378:metabolism* / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000595:Amino Acid Sequence; D001219:Aspartate Aminotransferases / Q000378:metabolism; D045744:Cell Line, Tumor; D004268:DNA-Binding Proteins / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D025261:Mitochondrial Proton-Translocating ATPases / Q000378:metabolism; D008969:Molecular Sequence Data; D009088:Mucoproteins; D009361:Neoplasm Invasiveness; D015513:Oncogene Proteins; D011506:Proteins / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology; D014157:Transcription Factors / Q000378:metabolism
chemical_list: C502296:AGR2 protein, human; C063898:DBP protein, human; D004268:DNA-Binding Proteins; D009088:Mucoproteins; D015513:Oncogene Proteins; D011506:Proteins; D014157:Transcription Factors; D001219:Aspartate Aminotransferases; D025261:Mitochondrial Proton-Translocating ATPases