title: Monoacylglycerol lipase (MAGL) knockdown inhibits tumor cells growth in colorectal cancer.
abstract: OBJECTIVE
  Obesity has been reported to increase the risk of colorectal cancer, which may due to aberrant lipid metabolism. And recently findings of monoacylglycerol lipase provide a novel evidence in the correlation of obesity and cancer. So in this study, we investigated the effect of MAGL in regulation of tumor growth in colorectal cancer.
  
  
  METHODS
  MAGL expression in tumor tissues was estimated, and then JZL184 and siRNA were used to knockdown the expression of MAGL in colorectal cancer cells. Cell viability and invasion were detected to estimate the influence of MAGL knocked down in vitro and vivo. Then cell proliferation, apoptosis, cell cycle transition and screening of candidate genes were performed for further exploring of the effect mediated by MAGL knocked down.
  
  
  RESULTS
  It was noted that the expression of MAGL was highly elevated in tumor tissues, however, it was found only significantly correlated with the BMI index. Tumor cells' growth and invasion was significantly inhibited in vitro and in vivo induced by pharmacological and siRNA mediated MAGL knocked down. Cell proliferation was reduced and apoptosis was increased. And two target genes Cyclin D1 and Bcl-2 seemed to be repressed by MAGL knocked down.
  
  
  CONCLUSIONS
  This study demonstrated colorectal cancer cells growth can be inhibited via knockdown of MAGL, which manipulate tumor cells proliferation and apoptosis by downregulation of Cyclin D1 and Bcl-2. It provides a novel therapeutic target in treatment of colorectal cancer and a further support for the correlation of obesity and colorectal cancer.
authors.affiliation: Department of General Surgery, Union Hospital affiliated to HuaZhong University of Science and Technology, WuHan 430022, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Ye
authors.affiliation: Department of General Surgery, Union Hospital affiliated to HuaZhong University of Science and Technology, WuHan 430022, China; Department of Systems Biology, Unit 950, The University of Texas M. D. Anderson Cancer Center, Houston, TX 77054, United States of America.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Department of Systems Biology, Unit 950, The University of Texas M. D. Anderson Cancer Center, Houston, TX 77054, United States of America.
authors.forename: Elena G
authors.initials: EG
authors.lastname: Seviour
authors.affiliation: Department of General Surgery, Union Hospital affiliated to HuaZhong University of Science and Technology, WuHan 430022, China.
authors.forename: Kai-Xiong
authors.initials: KX
authors.lastname: Tao
authors.affiliation: Department of General Surgery, Union Hospital affiliated to HuaZhong University of Science and Technology, WuHan 430022, China.
authors.forename: Xing-Hua
authors.initials: XH
authors.lastname: Liu
authors.affiliation: Department of General Surgery, Union Hospital affiliated to HuaZhong University of Science and Technology, WuHan 430022, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Ling
authors.affiliation: Department of General Surgery, Union Hospital affiliated to HuaZhong University of Science and Technology, WuHan 430022, China. Electronic address: yelinwuhan@yahoo.com.cn.
authors.forename: Jian-Ying
authors.initials: JY
authors.lastname: Chen
authors.affiliation: Department of General Surgery, Union Hospital affiliated to HuaZhong University of Science and Technology, WuHan 430022, China. Electronic address: yelinwuhan@sohu.com.
authors.forename: Guo-Bin
authors.initials: GB
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis; D015153:Blotting, Western; D002448:Cell Adhesion; D002453:Cell Cycle; D002465:Cell Movement; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000201:enzymology / Q000473:pathology / Q000517:prevention & control*; D019938:Cyclin D1 / Q000235:genetics / Q000378:metabolism; D005260:Female; D055785:Gene Knockdown Techniques; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D008994:Monoacylglycerol Lipases / Q000235:genetics* / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D019938:Cyclin D1; D008994:Monoacylglycerol Lipases