title: Degradation kinetics of metronidazole and olsalazine by bacteria in ascending colon and in feces of healthy adults.
abstract: OBJECTIVE
  To compare the degradation kinetics of metronidazole and olsalazine by the bacteria of ascending colon and the bacteria of feces of healthy adults.
  
  
  METHODS
  Contents of the ascending colon of seven healthy adults were collected under conditions simulating the bioavailability/bioequivalence studies in the fasted and in the fed states on a crossover basis. Material from the contents of the ascending colon was prepared by ultracentrifuging and diluting the precipitate with a volume of normal saline equivalent to that of the supernatant. Fecal material was prepared from feces of three healthy adults collected at two occasions that were separated by at least 6 months. Ex vivo drug degradation kinetics were evaluated under anaerobic conditions.
  
  
  RESULTS
  Mean half-lives of metronidazole degradation in material from the contents of the ascending colon collected in the fasted state and in fecal material were 16.1 and 2.4 min, respectively (p<0.001). The corresponding numbers for olsalazine were 57.8 and 9.2 min, respectively (p<0.001). Both compounds were stable in material from the contents of ascending colon collected in the fed state.
  
  
  CONCLUSIONS
  Compared with data in fecal material, degradation of metronidazole and olsalazine in material from the contents of the ascending colon is significantly slower and it becomes non-significant during the arrival of fresh food remnants in the region.
authors.affiliation: Laboratory of Biopharmaceutics and Pharmacokinetics, National and Kapodistrian, University of Athens, Athens, Greece. vertzoni@pharm.uoa.gr
authors.forename: Maria
authors.initials: M
authors.lastname: Vertzoni
authors.forename: Anders
authors.initials: A
authors.lastname: Carlsson
authors.forename: Bertil
authors.initials: B
authors.lastname: Abrahamsson
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Goumas
authors.forename: Christos
authors.initials: C
authors.lastname: Reppas
mesh_terms: D000328:Adult; D000636:Aminosalicylic Acids / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D000890:Anti-Infective Agents / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D001419:Bacteria / Q000378:metabolism*; D044682:Colon, Ascending / Q000382:microbiology; D005243:Feces / Q000382:microbiology; D005260:Female; D005765:Gastrointestinal Agents / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008297:Male; D008795:Metronidazole / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D008875:Middle Aged; D055815:Young Adult
chemical_list: D000636:Aminosalicylic Acids; D000890:Anti-Infective Agents; D005765:Gastrointestinal Agents; D008795:Metronidazole; C032587:olsalazine