title: [The development and application of solid-phase ELISA for the identification of avian influenza virus and its subtypes H5 and N3 in different biological samples].
abstract: The paper presents the results of research on the development of effective solid-phase ELISA methods for the identification of avian influenza virus (AIV) and typing its H5 and N3 subtypes in different biological samples. Optimized schemes for the production of goat antisera to matrix protein, hemagglutinin H5 and neuraminidase N3 of AIV were proposed. They were used to produce active and specific immunoglobulins and conjugates. Conditions of performing solid-phase ELISA with use of these preparations were optimized.
authors.forename: V M
authors.initials: VM
authors.lastname: Matveeva
authors.forename: Zh K
authors.initials: ZhK
authors.lastname: Koshemetov
authors.forename: S M
authors.initials: SM
authors.lastname: Mamadaliev
mesh_terms: D000818:Animals; D001717:Birds; D004797:Enzyme-Linked Immunosorbent Assay; D006041:Goats; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000276:immunology; D053124:Influenza A Virus, H5N1 Subtype / Q000145:classification / Q000302:isolation & purification*; D005585:Influenza in Birds / Q000175:diagnosis* / Q000821:virology; D009439:Neuraminidase / Q000276:immunology; D014763:Viral Matrix Proteins / Q000276:immunology
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus; C031540:M-protein, influenza virus; D014763:Viral Matrix Proteins; C109963:hemagglutinin, avian influenza A virus; D009439:Neuraminidase