title: Genotoxic effect of chronic exposure to DDT on lymphocytes, oral mucosa and breast cells of female rats.
abstract: The genotoxicity of some environmental contaminants may affect human health directly by damaging genetic material and thus plays an important role in cancer development. Xenoestrogens are one kind of environmental pollutants that may alter hormonal routes or directly affect DNA. The number of available biomarkers used to assess genetic risk and cancer is very extensive. The present study evaluated genotoxicity produced by the pesticide DDT on systemic and mammary gland cells obtained from adult female Wistar rats. Oral mucosa cells micronuclei were assessed; the comet assay in peripheral blood-isolated lymphocytes and mammary epithelial cells was also carried out. Additionally, oxidative stress was studied in mammary tissue through a lipid peroxidation assay. Our data showed an increase in lipid peroxidation, product of an increase in free oxygen radical levels, which leads to an oxidative stress status. Our results suggest that DDT is genotoxic, not only for lymphocytes but also to mammary epithelial cells.
authors.affiliation: Unit of Medical and Pharmaceutical Biotechnology, Center for Research and Assistance in Technology and Design of Jalisco, Normalistas 800, Guadalajara 44270, Mexico. acanales@ciatej.net.mx
authors.forename: Alejandro
authors.initials: A
authors.lastname: Canales-Aguirre
authors.forename: Eduardo
authors.initials: E
authors.lastname: Padilla-Camberos
authors.forename: Ulises
authors.initials: U
authors.lastname: Gómez-Pinedo
authors.forename: Hugo
authors.initials: H
authors.lastname: Salado-Ponce
authors.forename: Alfredo
authors.initials: A
authors.lastname: Feria-Velasco
authors.forename: Ruth
authors.initials: R
authors.lastname: De Celis
mesh_terms: D000818:Animals; D020552:Comet Assay; D003634:DDT / Q000633:toxicity*; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D005609:Free Radicals / Q000378:metabolism; D008214:Lymphocytes / Q000187:drug effects*; D008321:Mammary Glands, Animal / Q000187:drug effects* / Q000378:metabolism; D015162:Micronucleus Tests; D009061:Mouth Mucosa / Q000187:drug effects*; D051381:Rats
chemical_list: D005609:Free Radicals; D003634:DDT