title: Rapid on-site detection of Acidovorax avenae subsp. citrulli by gold-labeled DNA strip sensor.
abstract: Acidovorax avenae subsp. citrulli (AAC) is one of the most harmful diseases in cucurbit production. A rapid and sensitive DNA strip sensor was constructed based on gold nanoparticle-labeled oligonucleotide probes for the detection of AAC. Both the qualitative and semi-quantitative detections of target DNA were successfully achieved using the developed DNA strip sensor. The qualitative limit of detection (LOD) of the strip sensor was determined as 4 nM. The LOD for the semi-quantitative detection was calculated to be 0.48 nM in the range of 0-10 nM. The genomic DNA was detected directly using the DNA strip sensor without any further treatment. This DNA strip sensor is a potentially useful tool for rapid on-site DNA screening.
authors.affiliation: Chinese Academy of Inspection and Quarantine, Beijing 100029, PR China.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Zhao
authors.forename: Jie
authors.initials: J
authors.lastname: Lu
authors.forename: Wenwei
authors.initials: W
authors.lastname: Ma
authors.forename: Chuanlai
authors.initials: C
authors.lastname: Xu
authors.forename: Hua
authors.initials: H
authors.lastname: Kuang
authors.forename: Shuifang
authors.initials: S
authors.lastname: Zhu
mesh_terms: D001483:Base Sequence; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods / Q000706:statistics & numerical data; D042621:Comamonadaceae / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D015342:DNA Probes / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics*; D005516:Food Microbiology; D005638:Fruit / Q000382:microbiology; D006046:Gold; D053768:Metal Nanoparticles; D010935:Plant Diseases / Q000382:microbiology; D012639:Seeds / Q000382:microbiology
chemical_list: D015342:DNA Probes; D004269:DNA, Bacterial; D006046:Gold