title: Approaches to study differentiation and repair of human airway epithelial cells.
abstract: One of the main functions of the airway mucosa is to maintain a mechanical barrier at the air-surface interface and to protect the respiratory tract from external injuries. Differentiation of human airway epithelial cells (hAECs) to polarized airway mucosa can be reproduced in vitro by culturing the cells on microporous membrane at the air-liquid interface. Here, we describe approaches to study differentiation as well as repair of the hAECs by using a commercially available airway cell culture model called MucilAir™.
authors.affiliation: Laboratory of Clinical Investigation III, Faculty of Medicine, Department of Pediatrics, Geneva University Hospitals and University of Geneva, Foundation for Medical Research, 64 Avenue de la Roseraie, 1211, Geneva, Switzerland. Sophie.Crespin@hcuge.ch
authors.forename: Sophie
authors.initials: S
authors.lastname: Crespin
authors.forename: Marc
authors.initials: M
authors.lastname: Bacchetta
authors.forename: Song
authors.initials: S
authors.lastname: Huang
authors.forename: Tecla
authors.initials: T
authors.lastname: Dudez
authors.forename: Ludovic
authors.initials: L
authors.lastname: Wiszniewski
authors.forename: Marc
authors.initials: M
authors.lastname: Chanson
mesh_terms: D000818:Animals; D002450:Cell Communication / Q000502:physiology; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology*; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D016209:Interleukin-8 / Q000032:analysis / Q000096:biosynthesis; D051379:Mice; D008954:Models, Biological; D009077:Mucins / Q000032:analysis / Q000096:biosynthesis; D012107:Research Design; D020545:Respiratory Mucosa / Q000166:cytology / Q000378:metabolism*; D014945:Wound Healing / Q000502:physiology*
chemical_list: D016209:Interleukin-8; D009077:Mucins