title: Isolation and characterization of an oil palm constitutive promoter derived from a translationally control tumor protein (TCTP) gene.
abstract: We have characterized an oil palm (Elaeis guineensis Jacq.) constitutive promoter that is derived from a translationally control tumor protein (TCTP) gene. The TCTP promoter was fused transcriptionally with the gusA reporter gene and transferred to monocot and dicot systems in order to study its regulatory role in a transient expression study. It was found that the 5' region of TCTP was capable of driving the gusA expression in all the oil palm tissues tested, including immature embryo, embryogenic callus, embryoid, young leaflet from mature palm, green leaf, mesocarp and stem. It could also be used in dicot systems as it was also capable of driving gusA expression in tobacco leaves. The results indicate that the TCTP promoter could be used for the production of recombinant proteins that require constitutive expression in the plant system.
authors.affiliation: Advanced Biotechnology and Breeding Centre (ABBC), Biological Research Division, Malaysian Palm Oil Board (MPOB), P.O. Box 10620, 50720 Kuala Lumpur, Malaysia.
authors.forename: Subhi Siti
authors.initials: SS
authors.lastname: Masura
authors.forename: Ghulam Kadir Ahmad
authors.initials: GK
authors.lastname: Parveez
authors.forename: Leslie Low Eng
authors.initials: LL
authors.lastname: Ti
mesh_terms: D000595:Amino Acid Sequence; D028023:Arecaceae / Q000166:cytology / Q000235:genetics* / Q000502:physiology; D001483:Base Sequence; D019470:Biolistics; D015152:Blotting, Northern; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D035264:Flowers / Q000235:genetics / Q000502:physiology; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D017343:Genes, Plant / Q000235:genetics*; D017930:Genes, Reporter; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000235:genetics / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics*; D018517:Plant Roots / Q000235:genetics / Q000502:physiology; D030821:Plants, Genetically Modified; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics*; D018749:RNA, Plant / Q000235:genetics; D017422:Sequence Analysis, DNA; D013312:Stress, Physiological; D014026:Nicotiana / Q000235:genetics / Q000502:physiology
chemical_list: D018744:DNA, Plant; D010940:Plant Proteins; D018749:RNA, Plant