title: SDF1-induced antagonism of axonal repulsion requires multiple G-protein coupled signaling components that work in parallel.
abstract: SDF1 reduces the responsiveness of axonal growth cones to repellent guidance cues in a pertussis-toxin-sensitive, cAMP-dependent manner. Here, we show that SDF1's antirepellent effect can be blocked in embryonic chick dorsal root ganglia (DRGs) by expression of peptides or proteins inhibiting either Gα(i), Gα(q), or Gβγ. SDF1 antirepellent activity is also blocked by pharmacological inhibition of PLC, a common effector protein for Gα(q). We also show that SDF1 antirepellent activity can be mimicked by overexpression of constitutively active Gα(i), Gα(q), or Gα(s). These results suggest a model in which multiple G protein components cooperate to produce the cAMP levels required for SDF1 antirepellent activity.
authors.affiliation: Neuroscience Graduate Group, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, United States of America.
authors.forename: E Naomi
authors.initials: EN
authors.lastname: Twery
authors.forename: Jonathan A
authors.initials: JA
authors.lastname: Raper
mesh_terms: D000818:Animals; D001369:Axons; D054377:Chemokine CXCL12 / Q000502:physiology*; D002642:Chick Embryo; D019204:GTP-Binding Proteins / Q000378:metabolism*; D015398:Signal Transduction; D010738:Type C Phospholipases / Q000037:antagonists & inhibitors
chemical_list: D054377:Chemokine CXCL12; D010738:Type C Phospholipases; D019204:GTP-Binding Proteins