title: Effect of lectins from Diocleinae subtribe against oral Streptococci.
abstract: Surface colonization is an essential step in biofilm development. The ability of oral pathogens to adhere to tooth surfaces is directly linked with the presence of specific molecules at the bacterial surface that can interact with enamel acquired pellicle ligands. In light of this, the aim of this study was to verify inhibitory and antibiofilm action of lectins from the Diocleinaesubtribe against Streptococcus mutans and Streptococcus oralis. The inhibitory action against planctonic cells was assessed using lectins from Canavaliaensi formis (ConA), Canavalia brasiliensis (ConBr), Canavalia maritima (ConM), Canavalia gladiata (CGL) and Canavalia boliviana (ConBol). ConBol, ConBr and ConM showed inhibitory activity on S. mutans growth. All lectins, except ConA, stimulated significantly the growth of S. oralis. To evaluate the effect on biofilm formation, clarified saliva was added to 96-well, flat-bottomed polystyrene plates, followed by the addition of solutions containing 100 or 200 µg/mL of the selected lectins. ConBol, ConM and ConA inhibited the S. mutans biofilms. No effects were found on S. oralis biofilms. Structure/function analysis were carried out using bioinformatics tools. The aperture and deepness of the CRD (Carbohydrate Recognition Domain) permit us to distinguish the two groups of Canavalia lectins in accordance to their actions against S. mutans and S. oralis. The results found provide a basis for encouraging the use of plant lectins as biotechnological tools in ecological control and prevention of caries disease.
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, Brazil.
authors.forename: Theodora Thays Arruda
authors.initials: TT
authors.lastname: Cavalcante
authors.forename: Bruno
authors.initials: B
authors.lastname: Anderson Matias da Rocha
authors.forename: Victor
authors.initials: V
authors.lastname: Alves Carneiro
authors.forename: Francisco
authors.initials: F
authors.lastname: Vassiliepe Sousa Arruda
authors.forename: Antônia Sâmia
authors.initials: AS
authors.lastname: Fernandes do Nascimento
authors.forename: Nairley
authors.initials: N
authors.lastname: Cardoso Sá
authors.forename: Kyria Santiago
authors.initials: KS
authors.lastname: do Nascimento
authors.forename: Benildo
authors.initials: B
authors.lastname: Sousa Cavada
authors.forename: Edson
authors.initials: E
authors.lastname: Holanda Teixeira
mesh_terms: D018441:Biofilms / Q000187:drug effects*; D003208:Concanavalin A / Q000494:pharmacology; D037121:Plant Lectins / Q000494:pharmacology*; D013291:Streptococcus / Q000187:drug effects* / Q000254:growth & development*; D013295:Streptococcus mutans / Q000187:drug effects / Q000254:growth & development; D019533:Streptococcus oralis / Q000187:drug effects / Q000254:growth & development
chemical_list: D037121:Plant Lectins; D003208:Concanavalin A