title: In vitro cytotoxic evaluation of processed natural coral in human osteoblasts.
abstract: This study aimed to evaluate the in vitro cytotoxic effects of locally produced processed natural coral (PNC) using human osteoblasts (HOS). Cytotoxicity was not observed when HOS cells were cultured with PNC, as assessed by (3-(4,5-dimethylthiazol-2-yl)-2-5-diphenyl tetrazolium bromide; MTT) and Neutral Red (NR) assays at concentration up 200 mg/mL for up to 72 hours. Flow cytometry (FCM) analysis showed that PNC (200 mg/mL) did not decrease viability of HOS cells after 48 and 72 hours of treatment. In a cell attachment study, the HOS cells attached to the edge of the PNC disc, and later grew into the pores of the PNC disc. All results from these studies indicate that locally produced PNC material is noncytotoxic and favors the growth of HOS cells.
authors.affiliation: School of Dental Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia.
authors.forename: Nor Shamsuria
authors.initials: NS
authors.lastname: Omar
authors.forename: Thirumulu Ponnuraj
authors.initials: TP
authors.lastname: Kannan
authors.forename: Abdul Rashid
authors.initials: AR
authors.lastname: Ismail
authors.forename: Siti Fadilah
authors.initials: SF
authors.lastname: Abdullah
authors.forename: Abdul Rani
authors.initials: AR
authors.lastname: Samsudin
authors.forename: Suzina Sheikh Abdul
authors.initials: SS
authors.lastname: Hamid
mesh_terms: D000818:Animals; D037421:Anthozoa; D017209:Apoptosis; D001672:Biocompatible Materials / Q000633:toxicity; D002448:Cell Adhesion; D002460:Cell Line; D005434:Flow Cytometry; D006801:Humans; D008422:Materials Testing; D009499:Neutral Red / Q000737:chemistry; D010006:Osteoblasts / Q000166:cytology*; D013778:Tetrazolium Salts / Q000737:chemistry; D013844:Thiazoles / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D013778:Tetrazolium Salts; D013844:Thiazoles; D009499:Neutral Red; C022616:thiazolyl blue