title: Detecting UV-lesions in the genome: The modular CRL4 ubiquitin ligase does it best!
abstract: The DDB1-DDB2-CUL4-RBX1 complex serves as the primary detection device for UV-induced lesions in the genome. It simultaneously functions as a CUL4 type E3 ubiquitin ligase. We review the current understanding of this dual function ubiquitin ligase and damage detection complex. The DDB2 damage binding module is merely one of a large family of possible DDB1-CUL4 associated factors (DCAF), most of which are substrate receptors for other DDB1-CUL4 complexes. DDB2 and the Cockayne-syndrome A protein (CSA) function in nucleotide excision repair, whereas the remaining receptors operate in a wide range of other biological pathways. We will examine the modular architecture of DDB1-CUL4 in complex with DDB2, CSA and CDT2 focusing on shared architectural, targeting and regulatory principles.
authors.affiliation: Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, CH-4058 Basel, Switzerland.
authors.forename: Andrea
authors.initials: A
authors.lastname: Scrima
authors.forename: Eric S
authors.initials: ES
authors.lastname: Fischer
authors.forename: Gondichatnahalli M
authors.initials: GM
authors.lastname: Lingaraju
authors.forename: Kerstin
authors.initials: K
authors.lastname: Böhm
authors.forename: Simone
authors.initials: S
authors.lastname: Cavadini
authors.forename: Nicolas H
authors.initials: NH
authors.lastname: Thomä
mesh_terms: D044842:Cullin Proteins / Q000378:metabolism; D004249:DNA Damage; D004260:DNA Repair; D045643:DNA Repair Enzymes / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D015894:Genome, Human / Q000235:genetics / Q000528:radiation effects*; D006801:Humans; D008957:Models, Genetic; D009687:Nuclear Proteins / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: C114234:CUL4A protein, human; D044842:Cullin Proteins; C091435:DDB1 protein, human; C117669:DDB2 protein, human; D004268:DNA-Binding Proteins; C429530:DTL protein, human; C095472:ERCC8 protein, human; D009687:Nuclear Proteins; D014157:Transcription Factors; D044767:Ubiquitin-Protein Ligases; D045643:DNA Repair Enzymes