title: Mesenchymal progenitor cells from canine fetal tissues: yolk sac, liver, and bone marrow.
abstract: During fetal development, mesenchymal progenitor (MP) cells are co-localized in major hematopoietic territories, such as yolk sac (YS), bone marrow (BM), liver (LV), and others. Studies using mouse and human MP cells isolated from fetus have shown that these cells are very similar but not identical to adult mesenchymal stem cells (MSC). Their differentiation potential is usually restricted to production of highly committed osteogenic and chondrogenic precursors. Such properties of fetal MP cells can be very useful for tissue regeneration, when a great number of committed precursors are required. The objectives of this study were to isolate and characterize MP cells from canine YS, BM, and LV in early and late stages of fetal development. Gestational stage was identified, and cell culture conditions were evaluated for efficient isolation of canine MP cells. All canine fetal MP cells expressed vimentin, nestin, and CD44 proteins. Cytokeratin 18 expression was observed in BM- and LV-MP cells, and vascular endothelial (VE)-cadherin expression was observed only in YS-MP cells. A small number of MP cells (5%) from LV and YS expressed Oct3/4 protein. The differentiation potential of canine fetal MP cells varied significantly: YS- and BM-MP cells differentiated into bone and cartilage, whereas LV-MP cells differentiation was limited to osteogenic fate. None of the canine fetal MP cells were able to differentiate into adipose cells. Our data suggest that canine fetal MP cells are an appropriate in vitro model to study MP biology from hematopoietic territories and they are a source of committed osteogenic and chondrogenic precursors for regenerative medicine.
authors.affiliation: Department of Surgery, University of São Paulo, São Paulo, Brazil. criswenceslau@usp.br
authors.forename: Cristiane Valverde
authors.initials: CV
authors.lastname: Wenceslau
authors.forename: Maria Angélica
authors.initials: MA
authors.lastname: Miglino
authors.forename: Daniele Santos
authors.initials: DS
authors.lastname: Martins
authors.forename: Carlos Eduardo
authors.initials: CE
authors.lastname: Ambrósio
authors.forename: Nelson Foresto
authors.initials: NF
authors.lastname: Lizier
authors.forename: Graciela Conceição
authors.initials: GC
authors.lastname: Pignatari
authors.forename: Irina
authors.initials: I
authors.lastname: Kerkis
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002478:Cells, Cultured; D004285:Dogs; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism; D007381:Intermediate Filament Proteins / Q000378:metabolism; D053538:Keratin-18 / Q000378:metabolism; D008099:Liver / Q000166:cytology*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism* / Q000648:ultrastructure; D051379:Mice; D008819:Mice, Nude; D046529:Microscopy, Electron, Transmission; D009419:Nerve Tissue Proteins / Q000378:metabolism; D064231:Nestin; D060888:Real-Time Polymerase Chain Reaction; D014746:Vimentin / Q000378:metabolism; D015017:Yolk Sac / Q000166:cytology*
chemical_list: D018960:Hyaluronan Receptors; D007381:Intermediate Filament Proteins; D053538:Keratin-18; C577484:NES protein, human; D009419:Nerve Tissue Proteins; C577485:Nes protein, mouse; D064231:Nestin; D014746:Vimentin