title: Preparation and characterization of galactose-modified liposomes by a nonaqueous enzymatic reaction.
abstract: In this study, NOH (NOH = N-octadecyl-4-[(D-galactopyranosyl)oxy]-2,3,5,6-tetrahydroxy hexanamide) was enzymatically synthesized as a targeting molecule and incorporated into liposomes to prepare a liposome surface modified with galactose. Glycyrrhetinic-acid-loaded liposome (GA-LP) and glycyrrhetinic-acid-loaded liposome surface modified with galactose (NOH-GA-LP) were prepared by the ethanol-injection method. NOH-GA-LP was characterized by morphology, particle size, zeta potential, encapsulation efficiency, release in vitro, and stability. The size of spherical particles was in the range of 179-211 nm. Spherical particles exhibit a positive electrical charge (38.7 mV) and possess high encapsulation efficiency (91.3%) and show sustained release (72% over 48 hours) in vitro. This novel approach for the liposome surface modified with galactose by enzymatic synthesis is expected to provide potential application as a drug carrier for active targeted delivery to hepatocytes.
authors.affiliation: Department of Chinese Herbal Medicine, Guangdong, Guangzhou University of Chinese Medicine, Guangzhou, China.
authors.forename: Bo-hong
authors.initials: BH
authors.lastname: Guo
authors.forename: Yi
authors.initials: Y
authors.lastname: Cheng
authors.forename: Lv-ping
authors.initials: LP
authors.lastname: Lin
authors.forename: De-hui
authors.initials: DH
authors.lastname: Lin
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
mesh_terms: D000577:Amides / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry*; D005690:Galactose / Q000737:chemistry*; D006034:Glycyrrhetinic Acid / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry* / Q000648:ultrastructure; D015394:Molecular Structure; D010316:Particle Size; D013499:Surface Properties
chemical_list: D000577:Amides; D004337:Drug Carriers; D008081:Liposomes; D006034:Glycyrrhetinic Acid; C000360:caproamide; D005690:Galactose