title: Electroconductive polymeric nanowire templates facilitates in vitro C17.2 neural stem cell line adhesion, proliferation and differentiation.
abstract: Stem cells still remain one of the most exciting and lucrative options for treatment of a variety of nervous system disorders and diseases. Although there are neural stem cells present in adults, the ability of both the peripheral and central nervous system for self-repair is limited at best. As such, there is a great need for a tissue engineering approach to solve nervous system disorders and diseases. In this study, we have developed electrically conductive surfaces with controlled arrays of high aspect ratio nanowires for the growth and maintenance of neural stem cells. The nanowire surfaces were fabricated from polycaprolactone using a novel nanotemplating technique, and were coated with an electrically conductive polymer, polypyrrole. The polypyrrole-coated nanowire surfaces were characterized using scanning electron microscopy and X-ray photoelectron spectroscopy. Additionally, the surface resistance of polypyrrole-coated nanowire surfaces was measured. C17.2 neural stem cells were used to evaluate the efficacy of the polypyrrole-coated nanowire surfaces to promote cell adhesion, proliferation and differentiation. The results presented here indicate significantly higher cellular adhesion and proliferation on polypyrrole-coated nanowire surfaces as compared to control surfaces. The differentiation potential of polypyrrole nanowire surfaces was also evaluated by immunostaining key neuronal markers that are expressed when NSCs differentiate into their respective neural lineages.
authors.affiliation: School of Biomedical Engineering, Colorado State University, Fort Collins, CO 80523, USA.
authors.forename: Samuel
authors.initials: S
authors.lastname: Bechara
authors.forename: Lucas
authors.initials: L
authors.lastname: Wadman
authors.forename: Ketul C
authors.initials: KC
authors.lastname: Popat
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D055664:Electrochemical Techniques; D006801:Humans; D008297:Male; D008422:Materials Testing; D051379:Mice; D053770:Nanowires / Q000737:chemistry*; D009416:Nerve Regeneration / Q000502:physiology; D058953:Neural Stem Cells / Q000502:physiology* / Q000648:ultrastructure; D011091:Polyesters / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry; D013119:Spinal Cord Injuries / Q000503:physiopathology; D013499:Surface Properties
chemical_list: D011091:Polyesters; D011108:Polymers; D011758:Pyrroles; C016240:polycaprolactone; C067635:polypyrrole