title: Inhibition of PRAME expression causes cell cycle arrest and apoptosis in leukemic cells.
abstract: The preferentially expressed antigen of melanoma (PRAME) is known as a tumor-associated antigen, but its function in leukemia remains unclear. We investigated the function with small interfering RNA (siRNA)-induced knockdown of PRAME in a K562 cell line. After PRAME siRNA transfection, proliferation was suppressed and cell cycle analysis showed G(0)/G(1) arrest, followed by apoptosis. PRAME siRNA-treated cells also showed changes in the genes affecting erythroid differentiation. We examined the PRAME expression levels and the S phase population of 32 acute leukemia patients at the time of diagnosis and relapse. An increase of the S phase population was accompanied by an increase of PRAME expression at relapse. Our results suggest that PRAME plays an important role in disease progression in acute leukemia.
authors.affiliation: Department of Hematology, Tokyo Women's Medical University, Tokyo, Japan.
authors.forename: Norina
authors.initials: N
authors.lastname: Tanaka
authors.forename: Yan-Hua
authors.initials: YH
authors.lastname: Wang
authors.forename: Masayuki
authors.initials: M
authors.lastname: Shiseki
authors.forename: Minoko
authors.initials: M
authors.lastname: Takanashi
authors.forename: Toshiko
authors.initials: T
authors.lastname: Motoji
mesh_terms: D000951:Antigens, Neoplasm / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D002453:Cell Cycle / Q000187:drug effects* / Q000235:genetics; D018450:Disease Progression; D004353:Drug Evaluation, Preclinical; D015973:Gene Expression Regulation, Leukemic / Q000187:drug effects; D006801:Humans; D020014:K562 Cells; D007938:Leukemia / Q000235:genetics / Q000473:pathology*; D034741:RNA, Small Interfering / Q000494:pharmacology*
chemical_list: D000951:Antigens, Neoplasm; D000970:Antineoplastic Agents; C404944:PRAME protein, human; D034741:RNA, Small Interfering