title: Metabolites in contact with the rat digestive tract after ingestion of a phenolic-rich dietary fiber matrix.
abstract: Grape antioxidant dietary fiber (GADF) is a phenolic-rich dietary fiber matrix. The aim of this work was to determine which phenolic compounds come into contact with colonic epithelial tissue after the ingestion of GADF. By use of HPLC-ESI-MS/MS techniques phenolic metabolites were detected in feces, cecal content, and colonic tissue from rats. Free (epi)catechin (EC) was detected in all three sources, and more than 20 conjugated metabolites of EC were also detected in feces. Fourteen microbially derived phenolic metabolites were also identified in feces, cecal content, and/or colonic tissue. These results show that during transit along the digestive tract, proanthocyanidin oligomers and polymers are depolymerized into EC units. After ingestion of GADF, free EC and its conjugates, as well as free and conjugated microbially derived phenolic metabolites, come into contact with the intestine epithelium for more than 24 h and may be partly responsible for the positive influence of GADF on gut health.
authors.affiliation: Institute for Advanced Chemistry of Catalonia, CSIC (IQAC-CSIC), Jordi Girona 18-26, 08034 Barcelona, Spain.
authors.forename: Sonia
authors.initials: S
authors.lastname: Touriño
authors.forename: Jara
authors.initials: J
authors.lastname: Pérez-Jiménez
authors.forename: María Luisa
authors.initials: ML
authors.lastname: Mateos-Martín
authors.forename: Elisabet
authors.initials: E
authors.lastname: Fuguet
authors.forename: María Pilar
authors.initials: MP
authors.lastname: Vinardell
authors.forename: Marta
authors.initials: M
authors.lastname: Cascante
authors.forename: Josep Lluís
authors.initials: JL
authors.lastname: Torres
mesh_terms: D000818:Animals; D004043:Dietary Fiber / Q000378:metabolism*; D004435:Eating; D005243:Feces / Q000737:chemistry; D005260:Female; D041981:Gastrointestinal Tract / Q000378:metabolism*; D010636:Phenols / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D027843:Vitis / Q000737:chemistry / Q000378:metabolism
chemical_list: D004043:Dietary Fiber; D010636:Phenols