title: Copper-induced oxidative damage, antioxidant response and genotoxicity in Lycopersicum esculentum Mill. and Cucumis sativus L.
abstract: Adequate copper (Cu(2+)) concentrations are required for plants; however, at higher concentrations it can also cause multiple toxic effects. In the present study, lipid peroxidation, hydrogen peroxide levels as well as ascorbate peroxidase (APX: EC 1/11/1/11) and catalase (CAT: EC 1.11.1.6) activities were determined in Lycopersicum esculentum Mill. and Cucumis sativus L. seedlings after 7-day exposure to copper sulfate. In addition, DNA damage in these two crops was assessed by measuring micronucleus (MN) frequency and tail moments (TM) as determined by Comet assay. Inhibitory copper concentrations (EC(50): 30 and 5.5 ppm for L. esculentum and C. sativus, respectively) were determined according to dose-dependent root inhibition curves, and EC(50) and 2×EC(50) were applied. Malondialdehyde (MDA) and H(2)O(2) levels significantly increased in all groups studied. CAT activity increased in treatment groups of C. sativus. APX activity increased in L. esculentum seedlings due to 2×EC(50) treatment. Reductions in mitotic indices (MI) represented Cu(2+)dependent root growth inhibition in all treatment groups studied. According to TMs and MN frequencies, copper exposure induced significant DNA damage (p < 0.05) in all study groups, whereas the DNA damage induced was dose dependent in C. sativus roots. In conclusion, Cu(2+)induced oxidative damage, elevations in H(2)O(2) levels and alterations in APX and CAT activities, as well as significant DNA damage in nuclei of both study groups. To our knowledge, this is the first comparative and comprehensive study demonstrating the effects of copper on two different plant species at relevant cytotoxic concentrations at both biochemical and genotoxicity levels with multiple end points.
authors.affiliation: Institute of Transplantation and Gene Sciences, Baskent University, 06980 Kazan, Ankara, Turkey. odiseri@baskent.edu.tr
authors.forename: Özlem Darcansoy
authors.initials: ÖD
authors.lastname: İşeri
authors.forename: Didem Aksoy
authors.initials: DA
authors.lastname: Körpe
authors.forename: Erkan
authors.initials: E
authors.lastname: Yurtcu
authors.forename: Feride Iffet
authors.initials: FI
authors.lastname: Sahin
authors.forename: Mehmet
authors.initials: M
authors.lastname: Haberal
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D003300:Copper / Q000494:pharmacology*; D019327:Copper Sulfate / Q000494:pharmacology*; D018553:Cucumis sativus / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D004249:DNA Damage; D006861:Hydrogen Peroxide / Q000378:metabolism; D015227:Lipid Peroxidation; D018551:Solanum lycopersicum / Q000187:drug effects* / Q000378:metabolism; D008940:Mitotic Index; D009152:Mutagenicity Tests / Q000379:methods; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D036226:Seedlings / Q000187:drug effects / Q000378:metabolism
chemical_list: D000975:Antioxidants; D003300:Copper; D006861:Hydrogen Peroxide; D002374:Catalase; D019327:Copper Sulfate