title: Tumor cell markers in uveal melanoma.
abstract: Monoclonal antibodies to the melanoma-associated antigens HMB-45 and NKI/C3, and for S-100 protein were applied to archival tissue of 43 intraocular melanomas. Tn addition, the expression of the oncoproteins ras-p21 (ras 10) and mutated Ha-ras (E 184) as well as neu/erb-B2 (p185) were immunohistochemically evaluated. Incubation with antibodies to HMB-45 and NKI/C3 revealed consistently moderate to strong staining in all cases. Comparable ras-p21 immunostaining with normal epithelium observed in infiltrating components with a pronounced heterogeneous pattern, was particulary evident in epitheloid tumor cells. In melanomas of the spindle cell type B there was a tendency for patients with neu/erb-B2 positivity to have a worse prognosis. Using the chi-squared test for trend a significant correlation was found between S-100 reactivity, neu/erb-B2 amplification and the clinical outcome.
authors.affiliation: KARL FRANZENS UNIV GRAZ,INST PATHOL,GRAZ,AUSTRIA. KARL FRANZENS UNIV GRAZ,DEPT OPHTHALMOL,GRAZ,AUSTRIA. UNIV LEIPZIG,INST PATHOL ANAT,D-7010 LEIPZIG,GERMANY.
authors.forename: G
authors.initials: G
authors.lastname: Wirnsberger
authors.forename: R
authors.initials: R
authors.lastname: Kleinert
authors.forename: G
authors.initials: G
authors.lastname: Langmann
authors.forename: U
authors.initials: U
authors.lastname: Fuchs