title: Characterization of the interaction between farrerol and bovine serum albumin by fluorescence and circular dichroism.
abstract: The binding of farrerol to bovine serum albumin (BSA) in aqueous solution was investigated by fluorescence quenching spectra, synchronous fluorescence spectra, circular dichroism (CD) and the three-dimensional (3D) fluorescence spectra at pH 7.40. The results of fluorescence titration indicated that farrerol could quench the intrinsic fluorescence of BSA in a static quenching way. The cause of showing upward curvy patterns in Stern-Volmer plots was analyzed. The binding sites number n and binding constant K using fluorescence quenching equation at 310 K were calculated. The binding distance and the energy transfer efficiency between farrerol and BSA were also obtained according to the theory of Förster's non-radiation energy transfer. The effect of some metal ions on the binding constant of farrerol with BSA was also studied. The effect of farrerol on the conformation of BSA was analyzed using CD, synchronous fluorescence spectra and three-dimensional (3D) fluorescence spectra under experimental conditions. Furthermore, the fluorescence displacement experiments indicated that farrerol could bind to the site I of BSA.
authors.affiliation: College of Chemistry and Chemical Engineering, Luoyang Normal University, Luoyang 471022, China.
authors.forename: Daojin
authors.initials: D
authors.lastname: Li
authors.forename: Ye
authors.initials: Y
authors.lastname: Wang
authors.forename: Jianjun
authors.initials: J
authors.lastname: Chen
authors.forename: Baoming
authors.initials: B
authors.lastname: Ji
mesh_terms: D000818:Animals; D002417:Cattle; D002867:Chromones / Q000378:metabolism*; D002942:Circular Dichroism; D004365:Drugs, Chinese Herbal / Q000378:metabolism*; D008958:Models, Molecular; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
chemical_list: D002867:Chromones; D004365:Drugs, Chinese Herbal; C015881:farrerol; D012710:Serum Albumin, Bovine