title: Direct detection of RNAs in living cells using peptide-inserted Renilla luciferase.
abstract: In this study, non-engineered RNAs were detected in living cells using bioluminescence. Two types of probe were utilized: a peptide inserted RLuc (PI-RLuc) probe and a split-RNA probe. Incorporation of the PI-RLuc and split-RNA probes enabled the direct detection of RNA introduced into living cells.
authors.affiliation: Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta, Midori-ku, Yokohama, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Andou
authors.forename: Tamaki
authors.initials: T
authors.lastname: Endoh
authors.forename: Masayasu
authors.initials: M
authors.lastname: Mie
authors.forename: Eiry
authors.initials: E
authors.lastname: Kobatake
mesh_terms: D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D006801:Humans; D049410:Luciferases, Renilla / Q000737:chemistry*; D008856:Microscopy, Fluorescence / Q000379:methods*; D010455:Peptides / Q000737:chemistry*; D012313:RNA / Q000032:analysis*; D015347:RNA Probes / Q000737:chemistry; D048790:Renilla / Q000201:enzymology
chemical_list: D010455:Peptides; D015347:RNA Probes; D012313:RNA; D049410:Luciferases, Renilla