title: Insights into the genome of the xanthan-producing phytopathogen Xanthomonas arboricola pv. pruni 109 by comparative genomic hybridization.
abstract: The phytopathogenic bacterium Xanthomonas arboricola pv. pruni is the causal agent of Prunus Bacterial Spot disease that infects cultivated Prunus species and their hybrids. Furthermore, X. arboricola pv. pruni (Xap) plays a role in biotechnology since it produces xanthan gum, an important biopolymer used mainly in the food, oil, and cosmetics industry. To gain first insights into the genome composition of this pathovar, genomic DNA of X. arboricola pv. pruni strains was compared to the genomes of reference strains X. campestris pv. campestris B100 (Xcc B100) and X. campestris pv. vesicatoria 85-10 (Xcv 85-10) applying microarray-based comparative genomic hybridizations (CGH). The results implied that X. arboricola pv. pruni 109 lacks 6.67% and 5.21% of the genes present in the reference strains Xcc B100 and Xcv 85-10, respectively. Most of the missing genes were found to be organized in clusters and do not belong to the core genome of the two reference strains. Often they encode mobile genetic elements. Furthermore, the absence of gene clusters coding for the lipopolysaccharide (LPS) O-antigens of Xcc B100 and Xcv 85-10 indicates that the structure of the O-antigen of X. arboricola pv. pruni 109 differs from that of Xcc B100 and Xcv 85-10.
authors.affiliation: Departamento de Ciência e Tecnologia Agroindustrial, FAEM, Universidade Federal de Pelotas, CEP 96010-900, Caixa Postal 354, Pelotas/RS, Brazil. laurimayer@gmail.com
authors.forename: Laurí
authors.initials: L
authors.lastname: Mayer
authors.forename: Claire Tondo
authors.initials: CT
authors.lastname: Vendruscolo
authors.forename: Wladimir Padilha da
authors.initials: WP
authors.lastname: Silva
authors.forename: Frank-Jörg
authors.initials: FJ
authors.lastname: Vorhölter
authors.forename: Anke
authors.initials: A
authors.lastname: Becker
authors.forename: Alfred
authors.initials: A
authors.lastname: Pühler
mesh_terms: D055028:Comparative Genomic Hybridization; D016680:Genome, Bacterial / Q000235:genetics*; D019081:O Antigens / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D010935:Plant Diseases / Q000382:microbiology*; D027861:Prunus; D014974:Xanthomonas / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity
chemical_list: D019081:O Antigens