title: Occurrence of a conserved domain in ATP diphosphohydrolases from pathogenic organisms associated to antigenicity in human parasitic diseases.
abstract: A polypeptide (r78-117) belonging to the potato apyrase was identified as a conserved domain shared with apyrase-like proteins from distinct pathogenic organisms, and was obtained as a 6xHis tag polypeptide (r-Domain B). By ELISA, high IgG, and IgG1 and IgG2a subtypes levels were detected in BALB/c mice pre-inoculated with r-Domain B. In Schistosoma mansoni adult worm or Leishmania (V.) braziliensis promastigote preparation, anti-r-Domain B antibodies inhibit 22-72% of the phosphohydrolytic activities and when immobilized on Protein A-Sepharose immunoprecipitate 42-91% of them. Western blots of the immunoprecipitated resin-antibody-antigen complexes identified bands of mw similar to those predicted for parasite proteins. Total IgG and subclasses of patients with leishmaniasis or schistosomiasis exhibited cross-immunoreactivity with r-Domain B. Therefore, the domain B within both S. mansoni SmATPDase 2 (r156-195) and L. (V.) braziliensis NDPase (r83-122) are potentially involved in the host immune response, and also seem to be conserved during host and parasites co-evolution.
authors.affiliation: Departamento de Bioquímica, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, Juiz de Fora, MG, Brazil.
authors.forename: Ana Carolina Ribeiro Gomes
authors.initials: AC
authors.lastname: Maia
authors.forename: Michelle Lima
authors.initials: ML
authors.lastname: Detoni
authors.forename: Gabriane Nascimento
authors.initials: GN
authors.lastname: Porcino
authors.forename: Thais Vieira
authors.initials: TV
authors.lastname: Soares
authors.forename: Michelia Antônia
authors.initials: MA
authors.lastname: do Nascimento Gusmão
authors.forename: Melissa Regina
authors.initials: MR
authors.lastname: Fessel
authors.forename: Marcos José
authors.initials: MJ
authors.lastname: Marques
authors.forename: Maria Aparecida
authors.initials: MA
authors.lastname: Souza
authors.forename: Paulo Marcos Zech
authors.initials: PM
authors.lastname: Coelho
authors.forename: Juliana Assis Silva Gomes
authors.initials: JA
authors.lastname: Estanislau
authors.forename: Manoel Otávio
authors.initials: MO
authors.lastname: da Costa Rocha
authors.forename: Marcelo
authors.initials: M
authors.lastname: de Oliveira Santos
authors.forename: Priscila
authors.initials: P
authors.lastname: Faria-Pinto
authors.forename: Eveline Gomes
authors.initials: EG
authors.lastname: Vasconcelos
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology*; D000913:Antibodies, Protozoan / Q000276:immunology*; D001081:Apyrase / Q000037:antagonists & inhibitors / Q000276:immunology*; D016022:Case-Control Studies; D017124:Conserved Sequence / Q000276:immunology*; D003429:Cross Reactions / Q000276:immunology; D006801:Humans; D047468:Immunoprecipitation; D007892:Leishmania braziliensis / Q000201:enzymology / Q000276:immunology; D007896:Leishmaniasis / Q000097:blood / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008875:Middle Aged; D012550:Schistosoma mansoni / Q000201:enzymology / Q000276:immunology; D012552:Schistosomiasis / Q000097:blood / Q000276:immunology; D017386:Sequence Homology, Amino Acid; D014349:Trypanosoma cruzi / Q000201:enzymology / Q000276:immunology
chemical_list: D000909:Antibodies, Helminth; D000913:Antibodies, Protozoan; D001081:Apyrase