title: An alkyltransferase-like protein from Thermus thermophilus HB8 affects the regulation of gene expression in alkylation response.
abstract: Alkylation is a type of stress that is fatal to cells. However, cells have various responses to alkylation. Alkyltransferase-like (ATL) protein is a novel protein involved in the repair of alkylated DNA; however, its repair mechanism at the molecular level is unclear. DNA microarray analysis revealed that the upregulation of 71 genes because of treatment with an alkylating agent N-methyl-N'-nitro-N-nitrosoguanidine was related to the presence of TTHA1564, the ATL protein from Thermus thermophilus HB8. Affinity chromatography showed a direct interaction of purified TTHA1564 with purified RNA polymerase holoenzyme. The amino acid sequence of TTHA1564 is homologous to that of the C-terminal domain of Ada protein, which acts as a transcriptional activator. These results suggest that TTHA1564 might act as a transcriptional regulator. The results of DNA microarray analysis also implied that the alkylating agent induced oxidation stress in addition to alkylation stress.
authors.affiliation: Department of Biological Sciences, Graduate School of Science, Osaka University, 1-1 Machikaneyama-cho, Toyonaka, Osaka 560-0043, Japan.
authors.forename: Rihito
authors.initials: R
authors.lastname: Morita
authors.forename: Hisahiro
authors.initials: H
authors.lastname: Hishinuma
authors.forename: Hiromasa
authors.initials: H
authors.lastname: Ohyama
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Mega
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Ohta
authors.forename: Noriko
authors.initials: N
authors.lastname: Nakagawa
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Agari
authors.forename: Kenji
authors.initials: K
authors.lastname: Fukui
authors.forename: Akeo
authors.initials: A
authors.lastname: Shinkai
authors.forename: Seiki
authors.initials: S
authors.lastname: Kuramitsu
authors.forename: Ryoji
authors.initials: R
authors.lastname: Masui
mesh_terms: D019883:Alkyl and Aryl Transferases / Q000235:genetics / Q000378:metabolism*; D000477:Alkylating Agents / Q000494:pharmacology; D000478:Alkylation / Q000235:genetics; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D013312:Stress, Physiological / Q000235:genetics; D016963:Thermus thermophilus / Q000201:enzymology / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D000477:Alkylating Agents; D001426:Bacterial Proteins; D014157:Transcription Factors; D008769:Methylnitronitrosoguanidine; D019883:Alkyl and Aryl Transferases