title: Complex assembly, crystallization and preliminary X-ray crystallographic studies of the swine major histocompatibility complex molecule SLA-1*1502.
abstract: In order to illustrate the structure of the swine MHC class I (SLA-I) molecule and to evaluate the cytotoxic T lymphocyte (CTL) response against porcine reproductive and respiratory syndrome virus (PRRSV), the ternary complex of the SLA-I molecule termed SLA-1*1502 with β(2)-microglobulin and the CTL epitope TMPPGFELY (PRRSV-NSP9(TY9)) derived from PRRSV nonstructural protein 9 (residues 198-206) was assembled and crystallized. The crystal diffracted X-rays to 2.2 Å resolution and belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 66.1, b = 74.1, c = 98.6 Å; it contained one molecule in the asymmetric unit. The Matthews coefficient and the solvent content were calculated to be 2.74 Å(3) Da(-1) and 55.17%, respectively. The results will be helpful in obtaining insight into the structural basis of the presentation of viral epitopes by SLA-I.
authors.affiliation: Department of Microbiology and Immunology, College of Veterinary Medicine, China Agricultural University, Bejing 100094, People's Republic of China.
authors.forename: Xiaocheng
authors.initials: X
authors.lastname: Pan
authors.forename: Jianxun
authors.initials: J
authors.lastname: Qi
authors.forename: Nianzhi
authors.initials: N
authors.lastname: Zhang
authors.forename: Qirun
authors.initials: Q
authors.lastname: Li
authors.forename: Chunsheng
authors.initials: C
authors.lastname: Yin
authors.forename: Rong
authors.initials: R
authors.lastname: Chen
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.forename: Chun
authors.initials: C
authors.lastname: Xia
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray; D015395:Histocompatibility Antigens Class I / Q000737:chemistry* / Q000276:immunology; D008285:Major Histocompatibility Complex; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D013552:Swine / Q000276:immunology*
chemical_list: D015395:Histocompatibility Antigens Class I