title: The kinase DYRKIA regulates pre-mRNA splicing in spermatogonia and proliferation of spermatogonia and Sertoli cells by phosphorylating a spliceosomal component, SAP155, in postnatal murine testes.
abstract: SAP155 is an essential component of the spliceosome and its phosphorylation is required for splicing catalysis, but little is known concerning its function and regulation during spermatogenesis in postnatal murine testes. We report that inhibition of dual-specificity tyrosine-phosphorylation regulated kinase (DYRK) IA strongly suppressed the mitogen-stimulated SAP155 phosphorylation and constitutive splicing of IκB pre-mRNA as well as the proliferation of spermatogonial and Sertoli cells in cultures of the 6-day post partum testes and a spermatogonial cell line, but not in a Sertoli cell line. Our findings suggest that the active spliceosome, containing SAP155 phosphorylated by DYRKIA, performs pre-mRNA splicing in spermatogonia during testicular development.
authors.affiliation: Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University, Kumamoto 860-8555, Japan. etoko@gpo.kumamoto-u.ac.jp
authors.forename: Ko
authors.initials: K
authors.lastname: Eto
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Sonoda
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Abe
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002460:Cell Line; D049109:Cell Proliferation; D051357:Cyclin-Dependent Kinase 2 / Q000037:antagonists & inhibitors / Q000378:metabolism; D008297:Male; D051379:Mice; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D017346:Protein Serine-Threonine Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011714:Pyrans / Q000494:pharmacology; D012322:RNA Precursors / Q000378:metabolism*; D012326:RNA Splicing / Q000187:drug effects; D000072260:RNA Splicing Factors; D017413:Ribonucleoprotein, U2 Small Nuclear / Q000378:metabolism*; D012708:Sertoli Cells / Q000201:enzymology* / Q000378:metabolism; D013093:Spermatogonia / Q000201:enzymology* / Q000378:metabolism; D013141:Spiro Compounds / Q000494:pharmacology; D017381:Spliceosomes / Q000378:metabolism*; D013737:Testis / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism; D014230:Triazoles / Q000494:pharmacology; D000097603:Dyrk Kinases
chemical_list: D051357:Cyclin-Dependent Kinase 2; D010750:Phosphoproteins; D047428:Protein Kinase Inhibitors; D017346:Protein Serine-Threonine Kinases; D011505:Protein-Tyrosine Kinases; D011714:Pyrans; D012322:RNA Precursors; D000072260:RNA Splicing Factors; D017413:Ribonucleoprotein, U2 Small Nuclear; D013141:Spiro Compounds; D014230:Triazoles; D000097603:Dyrk Kinases; C103118:4,5,6,7-tetrabromo-2-azabenzimidazole; C491799:Sf3b1 protein, mouse; C553684:spliceostatin A; C495893:Cdk2 protein, mouse