title: Homo-DNA templated chemistry and its application to nucleic acid sensing.
abstract: We have investigated the homo-DNA templated Staudinger reduction of the profluorophore rhodamine azide and have applied this reaction to the detection of natural DNA with a hybrid homo-DNA/DNA molecular beacon. In this system the sensing and the reporting unit are bioorthogonal to each other which facilitates sequence design and increases fidelity.
authors.affiliation: Department of Chemistry and Biochemistry, University of Bern, Freiestrasse 3, CH-3012 Bern, Switzerland.
authors.forename: Matthias
authors.initials: M
authors.lastname: Stoop
authors.forename: Christian J
authors.initials: CJ
authors.lastname: Leumann
mesh_terms: D020029:Base Pairing; D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000032:analysis* / Q000737:chemistry* / Q000235:genetics; D009705:Nucleosides / Q000737:chemistry*; D015345:Oligonucleotide Probes / Q000737:chemistry / Q000235:genetics; D013050:Spectrometry, Fluorescence
chemical_list: D009705:Nucleosides; D015345:Oligonucleotide Probes; D004247:DNA