title: Colonic mast cells in controls and slow transit constipation patients.
abstract: BACKGROUND
  There is recent evidence that mast cells may play important roles in the gut, especially concerning visceral hypersensitivity and motor activity. However, most data are only available for clinical conditions characterised by diarrhoea, where MC have chiefly investigated in the mucosal layer of the colon and there is almost no information concerning constipation.
  
  
  OBJECTIVE
  To investigate mast cells distribution in all colonic layers in controls and severely constipated patients.
  
  
  METHODS
  Full-thickness specimens from colons of patients undergoing surgery for slow transit constipation (n=29), compared with controls, were obtained and the number of mast cells (evaluated by specific monoclonal antibodies) counted as a whole and in single colonic segments (caecum, ascending, transverse, descending and sigmoid).
  
  
  RESULTS
  Compared with controls, constipated patients revealed significantly higher number of mast cells, both as overall number and in single colonic segments. The distribution of mast cells resulted fairly homogeneous in the various segment of the large bowel, in both controls and patients, and no significant difference in the percentage of degranulated cells was found between groups.
  
  
  CONCLUSIONS
  Colonic mast cells display a homogeneous distribution within the viscus. This cell population is shown to increase in severely constipated patients, which might represent a mechanism trying to compensate for the impaired propulsive activity of these patients.
authors.affiliation: Gastroenterology & Hepatology Section, Department of Clinical and Experimental Medicine, University of Perugia, Piazza Menghini 1, San Sisto (Perugia), Italy. gabassot@tin.it
authors.forename: G
authors.initials: G
authors.lastname: Bassotti
authors.forename: V
authors.initials: V
authors.lastname: Villanacci
authors.forename: R
authors.initials: R
authors.lastname: Nascimbeni
authors.forename: M
authors.initials: M
authors.lastname: Cadei
authors.forename: S
authors.initials: S
authors.lastname: Manenti
authors.forename: G
authors.initials: G
authors.lastname: Sabatino
authors.forename: C A
authors.initials: CA
authors.lastname: Maurer
authors.forename: G
authors.initials: G
authors.lastname: Cathomas
authors.forename: B
authors.initials: B
authors.lastname: Salerni
mesh_terms: D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D016022:Case-Control Studies; D003106:Colon / Q000473:pathology* / Q000503:physiopathology; D003248:Constipation / Q000473:pathology* / Q000503:physiopathology; D005260:Female; D005772:Gastrointestinal Transit; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008407:Mast Cells / Q000378:metabolism* / Q000473:pathology*; D008875:Middle Aged
chemical_list: D000911:Antibodies, Monoclonal