title: Diversity and evolution of AbaR genomic resistance islands in Acinetobacter baumannii strains of European clone I.
abstract: To assess the diversity of AbaR genomic resistance islands in Acinetobacter baumannii European clone I (MLST clonal complex 1), we investigated 26 multidrug-resistant strains of this major clone isolated from hospitals in 21 cities of 10 European countries between 1984 and 2005. Each strain harbored an AbaR structure integrated at the same position in the chromosomal ATPase gene. AbaR3, including four subtypes based on variations in class 1 integron cassettes, and AbaR10 were found in 15 and 2 strains, respectively, whereas a new, unique AbaR variant was discovered in each of the other 9 strains. These new variants, designated AbaR11 to AbaR19 (19.8 kb to 57.5 kb), seem to be truncated derivatives of AbaR3, likely resulting from the deletions of its internal parts mediated by either IS26 elements (AbaR12 to AbaR19) or homologous recombination (AbaR11). AbaR3 was detected in all 10 strains isolated in 1984 to 1991, while AbaR11 to AbaR19 were carried only by strains isolated since 1997. Our results and those from previous publications suggest that AbaR3 is the original form of AbaR in European clone I, which may have provided strains of the lineage with a selective advantage facilitating their spread in European hospitals in the 1980s or before.
authors.affiliation: Laboratory of Bacterial Genetics, National Institute of Public Health, Šrobárova 48, 100 42 Prague, Czech Republic.
authors.forename: Lenka
authors.initials: L
authors.lastname: Krizova
authors.forename: Lenie
authors.initials: L
authors.lastname: Dijkshoorn
authors.forename: Alexandr
authors.initials: A
authors.lastname: Nemec
mesh_terms: D040981:Acinetobacter baumannii / Q000187:drug effects / Q000235:genetics*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics*; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D044404:Genomic Islands / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins