title: A pilot study: microbiological conditions of the oral cavity in minipigs for peri-implantitis models.
abstract: As peri-implantitis is an emerging problem, the development of validated animal models is mandatory. The aim of this pilot study was to provide a first step in describing the normal oral flora of minipigs. In five minipigs, samples of the oral flora were collected with sterile cotton swabs from the buccal gingiva of the lower jaw. Two swabs per animal were collected, followed by bacterial isolation under both aerobe and anaerobe conditions. Microbiological analyses included biochemical tests, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and 16S rDNA gene sequence analysis. A total of 61 taxa were detected, 14-21 different bacterial taxa from each minipig. Among the Gram-positive cocci, mainly staphylococcal and streptococcal species were identified. Different Actinomyces species were the most abundant taxa in the group of Gram-positive rods. Among the anaerobic bacteria, the Gram-negative genera Fusobacterium, Bacteroides and Prevotella were the most often observed taxa. This is the first study which begins to describe the normal oral flora in minipigs in cultures to allow for the detection of a broad spectrum. Several bacterial species identified are different from human ones. No specific species for peri-implantitis could be detected in that healthy sample.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Christian-Albrechts-University Kiel, Arnold-Heller-Str 16, 24105 Kiel, Germany. becker@mkg.uni-kiel.de
authors.forename: Stephan T
authors.initials: ST
authors.lastname: Becker
authors.forename: Christof
authors.initials: C
authors.lastname: Dörfer
authors.forename: Christian
authors.initials: C
authors.lastname: Graetz
authors.forename: Wiebke
authors.initials: W
authors.lastname: De Buhr
authors.forename: Jörg
authors.initials: J
authors.lastname: Wiltfang
authors.forename: Rainer
authors.initials: R
authors.lastname: Podschun
mesh_terms: D000818:Animals; D001421:Bacteria, Anaerobic / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004195:Disease Models, Animal; D005881:Gingiva / Q000382:microbiology*; D006095:Gram-Positive Cocci / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016987:Gram-Positive Rods / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D008297:Male; D057873:Peri-Implantitis / Q000382:microbiology*; D010865:Pilot Projects; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012016:Reference Values; D017422:Sequence Analysis, DNA; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013552:Swine; D013556:Swine, Miniature
chemical_list: D012336:RNA, Ribosomal, 16S