title: [Tissue culture and rapid propogation of seeds of Uyghur traditional herbal Capparis spinosa].
abstract: OBJECTIVE
  To optimize the tissue culture and rapid proliferation techniques of seeds of Capparis spinosa for producing large scale seedlings.
  
  
  METHODS
  The seeds of Capparis spinosa were collected as explants and cultivated in different MS media, which were from Turpan in Xinjiang. The optimum media were selected by adjusting the combinations of different hormone and concentration.
  
  
  RESULTS
  The best on institution of asepsis explants was rinsing for 8 hours and 0.1% HgCl2 for 12 minutes. The medium MS + 6-BA 0.6 mg/L + NAA 0.1 mg/L was suitable for primary and second culture. The medium MS + 6-BA 0.6 mg/L + 2,4-D 1.0 mg/L was suitable for proliferation, and the optimum medium of rooting was MS + IBA 0.8 mg / L + 300 mg/L activated carbon.
  
  
  CONCLUSIONS
  The rapid proliferation technique of seeds of Capparis spinosa can be used for producing large scale seedlings.
authors.affiliation: College of Pharmaceutical Sciences, Xinjiang Agricultural University, Urumqi 830052, China. xjmshj@sina.com
authors.forename: Sheng-Jun
authors.initials: SJ
authors.lastname: Ma
authors.forename: Ting
authors.initials: T
authors.lastname: Lu
authors.forename: Ai-Qin
authors.initials: AQ
authors.lastname: Zhang
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Ling
authors.initials: L
authors.lastname: Zhou
mesh_terms: D001947:Breeding; D031484:Capparis / Q000254:growth & development*; D002681:China; D003470:Culture Media; D004203:Disinfection / Q000379:methods; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development; D036226:Seedlings / Q000187:drug effects*; D012639:Seeds; D046509:Tissue Culture Techniques
chemical_list: D003470:Culture Media; D010937:Plant Growth Regulators