title: Identification of serum protein markers for breast cancer relapse with SELDI-TOF MS.
abstract: Surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) was used to screen serum samples to identify protein markers for early breast cancer relapse. We collected 67 serum samples from patients with breast cancer (24 preoperative; 23 postoperative without breast cancer relapse; 20 postoperative with breast cancer relapse). Eight protein peaks varied between the presurgical group and the postsurgical group without breast cancer relapse; 4 protein peaks were differentially expressed between the postsurgical patients without relapse and patients with relapse. The peak at 3964 m/z dropped after surgery and rebounded after relapse (P < 0.01). These results indicate that there are differences in serum protein expression among the three different groups of patients. SELDI-TOF MS could be used to screen blood samples for the early detection of relapse in primary breast cancer patients. Specifically, protein peak at 3964 m/z is a potential biomarker for the detection of early breast cancer relapse.
authors.affiliation: Department of Chemotherapy Center, Zhejiang Cancer Hospital, Hangzhou, Zhejiang 310022, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Lei
authors.forename: Xiao-Jia
authors.initials: XJ
authors.lastname: Wang
authors.forename: Zhi-Guo
authors.initials: ZG
authors.lastname: Zheng
authors.forename: Jian
authors.initials: J
authors.lastname: Huang
authors.forename: Wen-Ming
authors.initials: WM
authors.lastname: Cao
authors.forename: Zhan-Hong
authors.initials: ZH
authors.lastname: Chen
authors.forename: Xi-Ying
authors.initials: XY
authors.lastname: Shao
authors.forename: Ju-Fen
authors.initials: JF
authors.lastname: Cai
authors.forename: Wei-Wu
authors.initials: WW
authors.lastname: Ye
authors.forename: Hong-Yang
authors.initials: HY
authors.lastname: Lu
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood; D014408:Biomarkers, Tumor / Q000097:blood*; D001798:Blood Proteins / Q000032:analysis; D001943:Breast Neoplasms / Q000097:blood* / Q000175:diagnosis; D005260:Female; D006801:Humans; D008875:Middle Aged; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D015415:Biomarkers; D014408:Biomarkers, Tumor; D001798:Blood Proteins