title: The ganglioside GM(3) is raised in the sera and tissue of patients with bladder tumours.
abstract: An extraction procedure was developed which allowed the quantification of gangliosides from small volumes of sera (0.5 cm(3)) and samples of tissue (10 mg wet weight) from subjects with bladder cancer and from controls. The gangliosides were identified by high performance thin layer chromatography and mass spectrometry. In all samples the major ganglioside was GM(3) and amounts were elevated in both tissue and sera derived from tumour patients. The total lipid-bound sialic acid was greater in tumour tissue than in healthy bladder but was below the level of detection in all sera. We suggest that serum GM(3) may be of prognostic value in bladder cancer.
authors.affiliation: N MANCHESTER GRP HOSP,MANCHESTER,LANCS,ENGLAND. CHRISTIE HOSP & HOLT RADIUM INST,MANCHESTER M20 9BX,LANCS,ENGLAND.
authors.forename: M
authors.initials: M
authors.lastname: Slevin
authors.forename: B
authors.initials: B
authors.lastname: Costello
authors.forename: S
authors.initials: S
authors.lastname: Kumar
authors.forename: J
authors.initials: J
authors.lastname: Gaffney