title: Establishment and characterization of a testicular cell line from the half-smooth tongue sole, Cynoglossus semilaevis.
abstract: Spermatogenesis within the adult testis is an excellent system for studying stem cell renewal and differentiation, which is under the control of testicular somatic cells. In order to understanding spermatogenesis in the half-smooth tongue sole (Cynoglossus semilaevis) as a marine fish model of aquaculture importance, we established a cell line called CSGC from a juvenile gonad of this organism. CSGC is composed of fibroblast-like cells, retains a diploid karyotype of 42 chromosomes, lacks the heterogametic W chromosome, lacks a female specific marker and expresses the dmrt, a marker for testicular somatic cells. Therefore, CSGC appears to consist of testicular somatic cell cells. We show that this cell line is effective for infection by the turbot reddish body iridovirus and flounder lymphocystis disease virus as evidenced by the appearance of cytopathic effect and virus propagation in the virus-infected cells, and most convincingly, the observation of viral particles by electon microscopy, demonstrateing that CSGC is suitable to study interactions between virus and host cells. As a first fish testicular somatic cell line of the ZZ-ZW genetic sex determination system, CSGC will be a useful tool to study sex-related events and interactions between somatic cells and germ cells during spermatogenesis.
authors.affiliation: Yellow Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Qingdao, China, 266071.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.forename: Xianli
authors.initials: X
authors.lastname: Wang
authors.forename: Zhenxia
authors.initials: Z
authors.lastname: Sha
authors.forename: Changgeng
authors.initials: C
authors.lastname: Yang
authors.forename: Shanshan
authors.initials: S
authors.lastname: Liu
authors.forename: Na
authors.initials: N
authors.lastname: Wang
authors.forename: Song-Lin
authors.initials: SL
authors.lastname: Chen
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D018929:Cell Culture Techniques; D002460:Cell Line; D049109:Cell Proliferation; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism; D005412:Flatfishes / Q000235:genetics* / Q000821:virology; D020869:Gene Expression Profiling; D007497:Iridoviridae / Q000502:physiology / Q000648:ultrastructure; D007621:Karyotyping; D008297:Male; D019849:Sex Determination Processes / Q000235:genetics; D013091:Spermatogenesis; D013234:Stem Cells / Q000166:cytology / Q000821:virology; D013737:Testis / Q000166:cytology*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D015415:Biomarkers; C110547:DMRT1 protein; D029941:Fish Proteins; D014157:Transcription Factors