title: [Calculation of feto-maternal haemorrhage volume using various morphological parameters and various formulas].
abstract: Quantification of FMH is essential for determination of an accurate dose of anti-RhD Ig in antepartum and postpartum immunoprophylaxis of HDFN. Doses of anti-RhD are established with reference to the volume of RhD positive foetal red blood cells in the circulation of RhD negative voluntaries. Results obtained from flow cytometry, microscopic Kleihauer-Betke test or serological test indicate percentage of these cells.
  
  
  OBJECTIVE
  To compare results of FMH volume calculation using various formulas.
  
  
  METHODS
  EDTA blood samples from 51 mothers and 51 cord blood samples from their newborns were analyzed. Five formulas were based on following parameters: percentage of foetal red blood cells in the blood sample from mother, mother's body weight and Hct, maternal and foetal MCV.
  
  
  RESULTS
  The range of maternal Hct was 25.7-46.2% (mean 34.3%), ratio of foetal MCV to maternal MCV was 1.09-1.41 (mean 1.19), body weight of mothers 56-99 kg (mean 73.5 kg) and calculated volume of their blood 3600-7425 mi (mean 5100 ml). For 0.4% FMH we predicted 2, 3 or 4 doses of anti-RhD Ig 500 IU (100 microg) and 1, 2 or 3 doses of anti-RhD Ig 750 IU (150 microg) depending on the woman.
  
  
  CONCLUSIONS
  The formula with individual maternal and foetal morphological parameters is much more accurate for calculation of the volume of FMH and doses of anti-RhD Ig than often used formula with average values of the parameters.
authors.affiliation: Centrum Medyczne Kształcenia Podyplomowego w Warszawie, Zakład Biofizyki.
authors.forename: Agata
authors.initials: A
authors.lastname: Gielezynska
authors.forename: Jadwiga
authors.initials: J
authors.lastname: Fabijańska-Mitek
authors.forename: Marzena
authors.initials: M
authors.lastname: Debska
mesh_terms: D004911:Erythrocyte Volume; D005260:Female; D005331:Fetomaternal Transfusion / Q000097:blood* / Q000175:diagnosis / Q000628:therapy*; D006801:Humans; D007231:Infant, Newborn; D011247:Pregnancy; D018029:Rho(D) Immune Globulin / Q000008:administration & dosage
chemical_list: D018029:Rho(D) Immune Globulin