title: Functional histology of glioma vasculature by FTIR imaging.
abstract: Fourier-transform infrared (FTIR) imaging has been used to investigate brain tumor angiogenesis using a mice solid tumor model and bare-gold (∅ 25 nm) or BaSO(4) (∅ 500 nm) nanoparticles (NP) injected into blood vasculature. FTIR images of 20-μm-thick tissue sections were used for chemical histology of healthy and tumor areas. Distribution of BaSO(4)-NP (using the 1,218-1,159 cm(-1) spectral interval) revealed clearly all details of blood vasculature with morphological abnormalities of tumor capillaries, while Au-NP (using the 1,046-1,002 cm(-1) spectral interval) revealed also diffusion properties of leaky blood vessels. Diffusion of Au-NP out of vascular space reached 64 ± 29 μm, showing the fenestration of "leaky" tumor blood vessels, which should allow small NP (<100 nm, as for Au-NP) to diffuse almost freely, while large NP should not (as for BaSO(4)-NP in this study). Therefore, we propose to develop FTIR imaging as a convenient tool for functional molecular histology imaging of brain tumor vasculature, both for identifying blood capillaries and for determining the extravascular diffusion space offered by vessel fenestration.
authors.affiliation: Université de Bordeaux, CNRS UMR 5248 CBMN, 2 Rue Robert Escarpit, 33604 Pessac, France.
authors.forename: Razia
authors.initials: R
authors.lastname: Noreen
authors.forename: Raphael
authors.initials: R
authors.lastname: Pineau
authors.forename: Chia-Chi
authors.initials: CC
authors.lastname: Chien
authors.forename: Mariangela
authors.initials: M
authors.lastname: Cestelli-Guidi
authors.forename: Yeukuang
authors.initials: Y
authors.lastname: Hwu
authors.forename: Augusto
authors.initials: A
authors.lastname: Marcelli
authors.forename: Michel
authors.initials: M
authors.lastname: Moenner
authors.forename: Cyril
authors.initials: C
authors.lastname: Petibois
mesh_terms: D000818:Animals; D001466:Barium Sulfate / Q000737:chemistry; D001932:Brain Neoplasms / Q000098:blood supply / Q000473:pathology; D003287:Contrast Media / Q000737:chemistry; D005910:Glioma / Q000098:blood supply / Q000473:pathology; D006046:Gold / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry; D051379:Mice; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D003287:Contrast Media; D001466:Barium Sulfate; D006046:Gold