title: A novel indole alkaloid from deep-sea sediment metagenomic clone-derived Escherichia coli fermentation broth.
abstract: To explore secondary metabolites in deep-sea sediment metagenomic clone-derived Escherichia coli fermentation broth, different kinds of chromatography methods were used in the isolation procedures, while the structures of the isolated compounds were assigned based on the MS analysis and their (1)H and (13)C NMR spectra including 2D NMR techniques such as COSY, HMQC, and HMBC experiments. As a result, a novel compound was isolated and characterized as N-{1-[4-(acetylamino)phenyl]-3-hydroxy-1-(1H-indol-3-yl)propan-2-yl}-2,2-dichloroacetamide (1). In addition, eight known compounds were also obtained. Fatty acid amide hydrolase and monoacylglycerol lipase were used to screen analgesic activity, and the new compound showed analgesic activity to some extent in pharmacological test.
authors.affiliation: Medical College, Xiamen University, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.forename: Xi-Xiang
authors.initials: XX
authors.lastname: Tang
authors.forename: Min
authors.initials: M
authors.lastname: Zheng
authors.forename: Zhi-Wei
authors.initials: ZW
authors.lastname: Yi
authors.forename: Xiang
authors.initials: X
authors.lastname: Xiao
authors.forename: Ying-Kun
authors.initials: YK
authors.lastname: Qiu
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wu
mesh_terms: D000581:Amidohydrolases / Q000378:metabolism; D000700:Analgesics / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D004926:Escherichia coli / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D026121:Indole Alkaloids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D056186:Metagenomics; D015394:Molecular Structure; D008994:Monoacylglycerol Lipases / Q000378:metabolism; D019906:Nuclear Magnetic Resonance, Biomolecular; D009792:Oceans and Seas; D000099252:Fatty Acid Amide Hydrolases
chemical_list: D000581:Amidohydrolases; D000700:Analgesics; D026121:Indole Alkaloids; D008994:Monoacylglycerol Lipases; D000099252:Fatty Acid Amide Hydrolases; C559334:N-(1-(4-(acetylamino)phenyl)-3-hydroxy-1-(1H-indol-3-yl)propan-2-yl)-2,2-dichloroacetamide