title: Analysis of repetitive DNA in chromosomes by flow cytometry.
abstract: We developed a flow cytometry method, chromosome flow fluorescence in situ hybridization (FISH), called CFF, to analyze repetitive DNA in chromosomes using FISH with directly labeled peptide nucleic acid (PNA) probes. We used CFF to measure the abundance of interstitial telomeric sequences in Chinese hamster chromosomes and major satellite sequences in mouse chromosomes. Using CFF we also identified parental homologs of human chromosome 18 with different amounts of repetitive DNA.
authors.affiliation: Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, British Columbia, Canada.
authors.forename: Julie
authors.initials: J
authors.lastname: Brind'Amour
authors.forename: Peter M
authors.initials: PM
authors.lastname: Lansdorp
mesh_terms: D000818:Animals; D001483:Base Sequence; D002875:Chromosomes / Q000235:genetics*; D002887:Chromosomes, Human, Pair 18 / Q000235:genetics; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000235:genetics*; D004276:DNA, Satellite / Q000235:genetics; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D051379:Mice; D015345:Oligonucleotide Probes; D020135:Peptide Nucleic Acids; D012091:Repetitive Sequences, Nucleic Acid; D016615:Telomere / Q000235:genetics
chemical_list: D004276:DNA, Satellite; D015345:Oligonucleotide Probes; D020135:Peptide Nucleic Acids; D004247:DNA