title: Enhanced auditory neuron survival following cell-based BDNF treatment in the deaf guinea pig.
abstract: Exogenous neurotrophin delivery to the deaf cochlea can prevent deafness-induced auditory neuron degeneration, however, we have previously reported that these survival effects are rapidly lost if the treatment stops. In addition, there are concerns that current experimental techniques are not safe enough to be used clinically. Therefore, for such treatments to be clinically transferable, methods of neurotrophin treatment that are safe, biocompatible and can support long-term auditory neuron survival are necessary. Cell transplantation and gene transfer, combined with encapsulation technologies, have the potential to address these issues. This study investigated the survival-promoting effects of encapsulated BDNF over-expressing Schwann cells on auditory neurons in the deaf guinea pig. In comparison to control (empty) capsules, there was significantly greater auditory neuron survival following the cell-based BDNF treatment. Concurrent use of a cochlear implant is expected to result in even greater auditory neuron survival, and provide a clinically relevant method to support auditory neuron survival that may lead to improved speech perception and language outcomes for cochlear implant patients.
authors.affiliation: The Bionic Ear Institute, East Melbourne, Victoria, Australia. lpettingill@bionicear.org
authors.forename: Lisa N
authors.initials: LN
authors.lastname: Pettingill
authors.forename: Andrew K
authors.initials: AK
authors.lastname: Wise
authors.forename: Marilyn S
authors.initials: MS
authors.lastname: Geaney
authors.forename: Robert K
authors.initials: RK
authors.lastname: Shepherd
mesh_terms: D000464:Alginates / Q000494:pharmacology; D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000494:pharmacology* / Q000627:therapeutic use*; D002214:Capsules; D002470:Cell Survival / Q000187:drug effects; D019929:Cochlear Implantation; D003056:Cochlear Nerve / Q000187:drug effects* / Q000473:pathology*; D003638:Deafness / Q000188:drug therapy* / Q000473:pathology; D020723:Glucuronic Acid / Q000494:pharmacology; D006168:Guinea Pigs; D006603:Hexuronic Acids / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects / Q000473:pathology*; D051381:Rats; D012583:Schwann Cells / Q000187:drug effects / Q000637:transplantation*; D014162:Transfection
chemical_list: D000464:Alginates; D019208:Brain-Derived Neurotrophic Factor; D002214:Capsules; D006603:Hexuronic Acids; D020723:Glucuronic Acid