title: [The enhancing effect of Angelica dahurica extracts on absorption of baicalin--the active composition of Scutellaria].
abstract: To explore the mechanism of the absorption enhancement of Angelica dahurica extract (Ade), the absorption mechanism of baicalin in the Scutcllaria water extraction as well as the effect of Angelica dahurica extract on absorption of baicalin were investigated. In order to determine the main absorption site, everted intestinal sac model was used to study the effect of Angelica dahurica extract on the absorption of baicalin at duodenum, jejunum, ileum and colon. In situ single pass intestinal perfusion model was performed to study the absorption of various concentrations of baicalin and the effect of Angelica dahurica extract on the absorption of baicalin at the main absorption site. To authenticate the consequence of perfusion by getting the blood from the hepatic portal vein and determine the concentration of the baicalin in the blood. The result showed that baicalin could be absorbed at all of the four intestinal segments with increasing absorption amount per unit as follows: ileum > colon > jejunum > duodenum. The absorption ofbaicalin in the duodenum significantly increased with Angelica dahurica extract, thus, duodenum was chosen to be the studying site. Apparent permeability values (Papp) and absorption rate constant (Ka) of baicalin in the duodenum increased gradually with higher concentrations. When the concentration of baicalin rises to a certain degree, the absorption increase had a saturable process, the absorption of baicalin may be an active transportation. Baicalin may be not a substrate of P-gp as verapamil which had not significantly affected the Papp and Ka of baicalin. The absorption of baicalin in the duodenum significantly increased (P < 0.01) in the two models with Angelica dahurica extract and the concentration of baicalin in the blood from the hepatic portal vein showed that the Angelica dahurica extract can increase the absorption of baicalin.
authors.affiliation: Key Laboratory of Modern Preparation of Traditional Chinese Medicine, Ministry of Education, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.
authors.forename: Jing-yun
authors.initials: JY
authors.lastname: Zhu
authors.forename: Xin-li
authors.initials: XL
authors.lastname: Liang
authors.forename: Guang-fa
authors.initials: GF
authors.lastname: Wang
authors.forename: Guo-wei
authors.initials: GW
authors.lastname: Zhao
authors.forename: Zheng-gen
authors.initials: ZG
authors.lastname: Liao
authors.forename: Yun-chao
authors.initials: YC
authors.lastname: Cao
authors.forename: Xu-long
authors.initials: XL
authors.lastname: Chen
authors.forename: Ming
authors.initials: M
authors.lastname: Yang
mesh_terms: D029969:Angelica / Q000737:chemistry*; D000818:Animals; D004357:Drug Synergism; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D004386:Duodenum / Q000378:metabolism; D005419:Flavonoids / Q000302:isolation & purification / Q000493:pharmacokinetics*; D041743:Herb-Drug Interactions; D007408:Intestinal Absorption / Q000187:drug effects*; D007413:Intestinal Mucosa / Q000378:metabolism; D008297:Male; D010477:Perfusion; D010539:Permeability; D010946:Plants, Medicinal / Q000737:chemistry; D011169:Portal Vein / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D031363:Scutellaria / Q000737:chemistry*; D014700:Verapamil / Q000494:pharmacology
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids; C038044:baicalin; D014700:Verapamil