title: The host phosphoinositide 5-phosphatase SHIP2 regulates dissemination of vaccinia virus.
abstract: After fusing with the plasma membrane, enveloped poxvirus virions form actin-filled membranous protrusions, called tails, beneath themselves and move toward adjacent uninfected cells. While much is known about the host and viral proteins that mediate formation of actin tails, much less is known about the factors controlling release. We found that the phosphoinositide 5-phosphatase SHIP2 localizes to actin tails. Localization requires phosphotyrosine, Abl and Src family tyrosine kinases, and neural Wiskott-Aldrich syndrome protein (N-WASP) but not the Arp2/Arp3 complex or actin. Cells lacking SHIP2 have normal actin tails but release more virus. Moreover, cells infected with viral strains with mutations in the release inhibitor A34 release more virus but recruit less SHIP2 to tails. Thus, the inhibitory effects of A34 on virus release are mediated by SHIP2. Together, these data suggest that SHIP2 and A34 may act as gatekeepers to regulate dissemination of poxviruses when environmental conditions are conducive.
authors.affiliation: Microbiology and Molecular Genetics Graduate Program, Emory University School of Medicine, Atlanta, Georgia 30322, USA.
authors.forename: Shannon
authors.initials: S
authors.lastname: McNulty
authors.forename: Kimberly
authors.initials: K
authors.lastname: Powell
authors.forename: Christophe
authors.initials: C
authors.lastname: Erneux
authors.forename: Daniel
authors.initials: D
authors.lastname: Kalman
mesh_terms: D000818:Animals; D002460:Cell Line; D020552:Comet Assay; D006801:Humans; D051379:Mice; D000072183:Phosphatidylinositol-3,4,5-Trisphosphate 5-Phosphatases; D010744:Phosphoric Monoester Hydrolases / Q000502:physiology*; D034622:RNA Interference; D014616:Vaccinia virus / Q000502:physiology*
chemical_list: D010744:Phosphoric Monoester Hydrolases; C098706:INPPL1 protein, human; D000072183:Phosphatidylinositol-3,4,5-Trisphosphate 5-Phosphatases