title: 'The effect of micronutrients on superoxide dismutase in senescent fibroblasts'.
abstract: The specific activities of zinc/copper (Zn/Cu)-superoxide dismutase (SOD-1) and manganese (Mn)-superoxide dismutase (SOD-2) were assayed in young passage 5 fibroblasts and in serially subcultured cells that were characterized as senescent at passages 15-35. SOD-1 and SOD-2 activities did not significantly change in senescent and young cells cultured in either routine medium [minimum essential medium 1 (MEM1)], or in Zn, Cu and Mn supplemented medium (MEM2) containing normal human plasma levels of the cations. SOD-1 and SOD-2 activities, however, underwent parallel progressive significant activity increases in senescent passage 20 and 25 cells, which peaked in value in passage 30 and 35 cells subcultured in supplemented medium (MEM3) containing triple human plasma levels of the cations. Concurrently, superoxide radical generation rates underwent progressive significant increases in senescent passage 15-25 cells, which peaked in value in passage 30 and 35 cells subcultured in MEM1 or MEM2. These rates, however, were significantly lowered in senescent cells subcultured in MEM3. We infer that it was only possible to significantly stimulate SOD-1 and SOD-2 activities in senescent MEM3 cultured cells enabling them to combat oxidative stress.
authors.affiliation: Chair of Medical and Molecular Genetics, Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Yazeed A
authors.initials: YA
authors.lastname: Al-Sheikh
authors.forename: Hazem K
authors.initials: HK
authors.lastname: Ghneim
mesh_terms: D002412:Cations / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects / Q000502:physiology*; D003300:Copper / Q000494:pharmacology; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects* / Q000502:physiology; D024981:Glycogen Phosphorylase / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D045022:Lactate Dehydrogenases / Q000187:drug effects / Q000378:metabolism; D008345:Manganese / Q000494:pharmacology; D018977:Micronutrients / Q000494:pharmacology*; D025221:Phosphofructokinases / Q000187:drug effects / Q000378:metabolism; D012867:Skin / Q000166:cytology; D013482:Superoxide Dismutase / Q000187:drug effects* / Q000378:metabolism; D000072105:Superoxide Dismutase-1; D013481:Superoxides / Q000378:metabolism; D015032:Zinc / Q000494:pharmacology; D000099156:Superoxide Dismutase 2
chemical_list: D002412:Cations; D003300:Copper; D003470:Culture Media; D024981:Glycogen Phosphorylase; D045022:Lactate Dehydrogenases; D008345:Manganese; D018977:Micronutrients; D025221:Phosphofructokinases; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; D013481:Superoxides; D015032:Zinc; D000099156:Superoxide Dismutase 2; C000606290:SOD1 protein, human