title: Influence of DNA-binding on the photochromic equilibrium of a chromene derivative.
abstract: The photochromic properties of a chromene derivative, which contains a positively charged substituent, were investigated in the presence of ct DNA. Upon irradiation in the absence of DNA the colored quinoid open form is highly unstable and rapidly reverts back to the initial closed form (bleaching). In the presence of DNA the open form is significantly stabilized, presumably because of the association with the macromolecule. The kinetics of the bleaching reaction of the bound ligand were determined and employed to estimate the binding constant.
authors.affiliation: A.N. Nesmeyanov Institute of Organoelement Compounds of the Russian Academy of Sciences, 28 Vavilova St., 119991, Moscow, Russian Federation. paramonov@ineos.ac.ru
authors.forename: Sergey V
authors.initials: SV
authors.lastname: Paramonov
authors.forename: Vladimir
authors.initials: V
authors.lastname: Lokshin
authors.forename: Heiko
authors.initials: H
authors.lastname: Ihmels
authors.forename: Olga A
authors.initials: OA
authors.lastname: Fedorova
mesh_terms: D001578:Benzopyrans / Q000737:chemistry*; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D007536:Isomerism; D007700:Kinetics; D013056:Spectrophotometry, Ultraviolet; D014466:Ultraviolet Rays
chemical_list: D001578:Benzopyrans; D004247:DNA