title: Analysis of RNA from brush cytology detects changes in B2M, CYP1B1 and KRT17 levels with OSCC in tobacco users.
abstract: RNA expression analysis of oral keratinocytes can be used to detect early oral cancer, but a limitation is the inability to obtain high quality RNA from oral tissue without using biopsies. While oral cytology cell samples can be obtained from patients in a minimally invasive manner, they have not been validated for quantitative analysis of RNA expression. Earlier we showed RNA from brush cytology of hamster Oral Squamous Cell Carcinoma (OSCC) demonstrated differential expression of B2M and CYP1B1 using real time RT-PCR in a dibenz[a,I]pyrene, tobacco carcinogen, induced model of this disease. Here we show reproducibility of this approach to measuring gene expression in humans. Cytology brush samples from 12 tobacco and betel related OSCC and 17 nonmalignant oral lesions revealed B2M mRNA was enriched in tumor samples while CYP1B1 mRNA was reduced, similar to what was seen in the model system. Additionally, we showed that KRT17 mRNA, a gene linked to OSCC in another brush cytology study, was also enriched in OSCC versus nonmalignant lesions, again supporting the promise of using RNA from brush oral cytology to reproducibly monitor oral gene expression.
authors.affiliation: Department of Oral and Maxillofacial Surgery, College of Dentistry, and University of Illinois at Chicago, 801 South Paulina Street, Chicago, IL 60610, USA.
authors.forename: Antonia
authors.initials: A
authors.lastname: Kolokythas
authors.forename: Joel L
authors.initials: JL
authors.lastname: Schwartz
authors.forename: Kristen B
authors.initials: KB
authors.lastname: Pytynia
authors.forename: Suchismita
authors.initials: S
authors.lastname: Panda
authors.forename: Mike
authors.initials: M
authors.lastname: Yao
authors.forename: Brian
authors.initials: B
authors.lastname: Homann
authors.forename: Herve Y
authors.initials: HY
authors.lastname: Sroussi
authors.forename: Joel B
authors.initials: JB
authors.lastname: Epstein
authors.forename: Sara C
authors.initials: SC
authors.lastname: Gordon
authors.forename: Guy R
authors.initials: GR
authors.lastname: Adami
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000235:genetics / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D006224:Cricetinae; D065633:Cytochrome P-450 CYP1B1; D005260:Female; D006801:Humans; D053537:Keratin-17 / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism; D012334:RNA, Neoplasm / Q000378:metabolism; D015203:Reproducibility of Results; D012907:Smoking / Q000009:adverse effects*; D055815:Young Adult; D001613:beta 2-Microglobulin / Q000235:genetics / Q000378:metabolism*
chemical_list: D053537:Keratin-17; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D001613:beta 2-Microglobulin; D001189:Aryl Hydrocarbon Hydroxylases; C585496:CYP1B1 protein, human; D065633:Cytochrome P-450 CYP1B1