title: Efficient adenovirus-mediated gene transfer into human cancer cell lines derived from digestive tract.
abstract: The efficiency of gene transfer into human cancer cells from digestive tract was evaluated using a replication-deficient recombinant adenovirus (Ad) vector harboring a lacZ gene of E. coli as a reporter gene (AxCALacZ). Average percent X-gal staining of esophageal cancer cell lines was 46%, that of gastric cancer cell lines 82% and that of colon cancer cell lines 70% at 3 days after Ad vector infection. X-gal staining in vitro continued 2 months after infection. By the direct injection of adenovirus vector to the tumors in nude mice, a certain percentage of tumor cells was stained by the X-gal. Colon26 cell line infected with AxCALacZ was implanted in BALB/c mice immunized with AxCALacZ, and tumor growth was suppressed. We presume this was due to anti-adenoviral immunity.
authors.affiliation: NAGOYA UNIV,SCH MED,DEPT IMMUNOL,SHOWA KU,NAGOYA,AICHI 466,JAPAN. NAGOYA UNIV,SCH MED,DEPT SURG 2,SHOWA KU,NAGOYA,AICHI 466,JAPAN. FUJITA HLTH UNIV,SCH MED,DEPT SURG,HISAI CITY,MIE 51412,JAPAN. UNIV TOKYO,INST MED SCI,MINATO KU,TOKYO 108,JAPAN.
authors.forename: H
authors.initials: H
authors.lastname: Sekiguchi
authors.forename: K
authors.initials: K
authors.lastname: Isobe
authors.forename: S
authors.initials: S
authors.lastname: Akiyama
authors.forename: H
authors.initials: H
authors.lastname: Yi
authors.forename: H
authors.initials: H
authors.lastname: Takeshita
authors.forename: T
authors.initials: T
authors.lastname: Watanabe
authors.forename: I
authors.initials: I
authors.lastname: Saito
authors.forename: Y
authors.initials: Y
authors.lastname: Kasai
authors.forename: K
authors.initials: K
authors.lastname: Ito
authors.forename: Y
authors.initials: Y
authors.lastname: Kanegae
authors.forename: I
authors.initials: I
authors.lastname: Nakashima
authors.forename: H
authors.initials: H
authors.lastname: Takagi