title: Combined Cerenkov luminescence and nuclear imaging of radioiodine in the thyroid gland and thyroid cancer cells expressing sodium iodide symporter: initial feasibility study.
abstract: Radioiodine (RI) such as (131)I or (124)I, can generate luminescent emission and be detected with an optical imaging (OI) device. To evaluate the possibility of a novel Cerenkov luminescence imaging (CLI) for application in thyroid research, we performed feasibility studies of CLI by RI in the thyroid gland and human anaplastic thyroid carcinoma cells expressing sodium iodide symporter gene (ARO-NIS). For in vitro study, FRTL-5 and ARO-NIS were incubated with RI, and the luminometric and CLI intensity was measured with luminometer and OI device. Luminescence intensity was compared with the radioactivity measured with γ-counter. In vivo CLI of the thyroid gland was performed in mice after intravenous injection of RI with and without thyroid blocking. Mice were implanted with ARO-NIS subcutaneously, and CLI was performed with injection of (124)I. Small animal PET or γ-camera imaging was also performed. CLI intensities of thyroid gland and ARO-NIS were quantified, and compared with the radioactivities measured from nuclear images (NI). Luminometric assay and OI confirmed RI uptake in the cells in a dose-dependent manner, and luminescence intensity was well correlated with radioactivity of the cells. CLI clearly demonstrated RI uptake in thyroid gland and xenografted ARO-NIS cells in mice, which was further confirmed by NI. A strong positive correlation was observed between CLI intensity and radioactivity assessed by NI. We successfully demonstrated dual molecular imaging of CLI and NI using RI both in vitro and in vivo. CLI can provide a new OI strategy in preclinical thyroid studies.
authors.affiliation: Department of Nuclear Medicine, Kyungpook National University School of Medicine, Daegu 700-422, Korea.
authors.forename: Shin Young
authors.initials: SY
authors.lastname: Jeong
authors.forename: Mi-Hye
authors.initials: MH
authors.lastname: Hwang
authors.forename: Jung Eun
authors.initials: JE
authors.lastname: Kim
authors.forename: Sungmin
authors.initials: S
authors.lastname: Kang
authors.forename: Jeong Chan
authors.initials: JC
authors.lastname: Park
authors.forename: Jeongsoo
authors.initials: J
authors.lastname: Yoo
authors.forename: Jeoung-Hee
authors.initials: JH
authors.lastname: Ha
authors.forename: Sang-Woo
authors.initials: SW
authors.lastname: Lee
authors.forename: Byeong-Cheol
authors.initials: BC
authors.lastname: Ahn
authors.forename: Jaetae
authors.initials: J
authors.lastname: Lee
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D003952:Diagnostic Imaging / Q000379:methods; D005260:Female; D006801:Humans; D007457:Iodine Radioisotopes; D049449:Luminescence; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D018613:Microscopy, Confocal; D010865:Pilot Projects; D011877:Radionuclide Imaging; D013047:Specific Pathogen-Free Organisms; D027981:Symporters / Q000096:biosynthesis*; D013961:Thyroid Gland / Q000000981:diagnostic imaging* / Q000378:metabolism*; D014183:Transplantation, Heterologous; D000099209:Sodium-Iodide Symporters
chemical_list: D007457:Iodine Radioisotopes; D027981:Symporters; D000099209:Sodium-Iodide Symporters