title: Therapeutic effect of anti-amyloid β peptide single-chain antibody gene mediated by adeno-associated virus on animal Alzheimer's disease.
abstract: OBJECTIVE
  To investigate the therapeutic effect of recombinant adeno-associated virus carrying anti-amyloid β peptide single-chain antibody gene on Alzheimer's disease (AD) in animal models.
  
  
  METHODS
  The recombinant adeno-associated viruses were injected to the leg muscle of mutant amyloid precursor protein transgenic AD mice. The latency of the mice in Morris water maze was tested before and 3, 7, 10 months after drug administration. The animal brains were harvested 10 months after drug administration and sectioned for amyloid plaques staining.
  
  
  RESULTS
  The learning and memory abilities of AD model mice were improved significantly 3 months after gene drug administration. Ten months after gene therapy, the numbers of amyloid plaque in hippocampus of model mice decreased.
  
  
  CONCLUSIONS
  The adeno-associated virus carrying anti-amyloid β peptide single-chain antibody gene has therapeutic effect on AD in model mice.
authors.affiliation: Department of Nuclear Medicine, PUMC Hospital, CAMS and PUMC, Beijing 100730, China. jiongcai@sina.com
authors.forename: Cai
authors.initials: C
authors.lastname: Jiong
authors.forename: Niu
authors.initials: N
authors.lastname: Na
authors.forename: Li
authors.initials: L
authors.lastname: Fang
authors.forename: Wang
authors.initials: W
authors.lastname: Shi-zhen
mesh_terms: D000544:Alzheimer Disease / Q000628:therapy*; D016564:Amyloid beta-Protein Precursor / Q000276:immunology*; D000818:Animals; D000229:Dependovirus / Q000235:genetics*; D004195:Disease Models, Animal; D005260:Female; D015316:Genetic Therapy; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D057127:Single-Chain Antibodies / Q000235:genetics*
chemical_list: D016564:Amyloid beta-Protein Precursor; D057127:Single-Chain Antibodies