title: Detection of somatic mutations of the bcl-2 oncogene in B cell lymphomas with the t(14;18).
abstract: The incidence of mutations within the first 582 bp of the open reading frame of the bcl-2 gene, has been investigated in presentation lymph node samples, from 7 cases with follicular non-Hodgkin's lymphoma (NHL), 1 case with centroblactic NHL, the DOHH, cell line derived from the immunoblastic transformation of a follicular NHL and one case with benign follicular hyperplasia. A total number of 43 point mutations within the examined portion of the bcl-2 gene were detected in the cases analysed including the DOHH, cell line. Similar analysis of peripheral blood lymphocytes from 2 normal individuals that lacked the t(14;18), revealed no mutations in one case and a single 101 bp A-->G transition in clone, in the other. Missense mutations were detected in 7/8 NHLs, the DOHH2 cell line and the case of benign follicular hyperplasia. There was a significantly higher frequency of mutations within the region corresponding to the BH1, one of the two known functional domains, of the bcl-2 protein. The same position, 445 bp of the bcl-2 gene, was found to be involved in missense mutations affecting the DOHH2 cell line and 3 cases with follicular NHL.
authors.affiliation: UNIV LONDON ST BARTHOLOMEWS HOSP MED COLL,ICRF DEPT,ICRF DEPT MED ONCOL,LONDON EC1M 6BQ,ENGLAND. ST BARTHOLOMEWS HOSP,DEPT HISTOPATHOL,LONDON,ENGLAND.
authors.forename: V
authors.initials: V
authors.lastname: Pappa
authors.forename: S
authors.initials: S
authors.lastname: Wilkes
authors.forename: A
authors.initials: A
authors.lastname: Norton
authors.forename: S
authors.initials: S
authors.lastname: Phillips
authors.forename: A
authors.initials: A
authors.lastname: Rohatiner
authors.forename: T
authors.initials: T
authors.lastname: Lister
authors.forename: B
authors.initials: B
authors.lastname: Young