title: PREPARATIVE ISOLATION AND PURIFICATION OF CHEMICAL CONSTITUENTS OF BELAMCANDA BY MPLC, HSCCC AND PREP-HPLC.
abstract: Combined with medium-pressure liquid chromatography (MPLC) and preparative high-pressure liquid chromatography (Prep-HPLC), high-speed countercurrent chromatography (HSCCC) was successfully applied for separation and purification of isoflavonoids from the extract of belamcanda. HSCCC separation was performed on a two-phase solvent system composed of methyl tert-butyl ether -ethyl acetate - n-butyl alcohol - acetonitrile -0.1% aqueous trifluoroacetic acid at a volume radio of 1:2:1:1:5. Semi-purified peak fractions from HSCCC separation were further purified by Prep-HPLC. Nine well-separated fractions were analyzed by HPLC-UV absorption spectrometry to determine their purities and characterized with ESI-MS(n). Except for peaksland VII (unknown) seven compounds were identified as apocynin (peak II), mangiferin (peak III), 7-O-methylmangiferin (peak IV), hispidulin (peak V), 3'-hydroxyltectoridin (peak VI), iristectorin B (peak VII), isoiridin (peak IX).
authors.affiliation: Institute of Analytical Chemistry, School of Chemistry and Environment, South China Normal University, Guangzhou, 510006.China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Wang
authors.forename: Yong
authors.initials: Y
authors.lastname: Liang
authors.forename: Cuilin
authors.initials: C
authors.lastname: Peng
authors.forename: Huichun
authors.initials: H
authors.lastname: Xie
authors.forename: Man
authors.initials: M
authors.lastname: Pan
authors.forename: Tianyou
authors.initials: T
authors.lastname: Zhang
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Ito