title: Efficient hydrolytic reaction of an acetate ester with fungal lipase in a liquid-liquid interface bioreactor (L-L IBR) using CaCO₃-coated ballooned microsphere.
abstract: In a liquid-liquid interface bioreactor using a CaCO₃-coated ballooned microsphere, 2-ethylhexyl acetate was efficiently hydrolyzed to 2-ethyl-1-hexanol with Absidia coerulea NBRC 4423 compared with using talc-coated or non-coated ballooned microsphere. It was assumed that CaCO₃ brought about stabilization of lipase by Ca²⁺ and maintenance of medium pH.
authors.affiliation: Genome Biotechnology Laboratory, Kanazawa Institute of Technology, 3-1 Yatsukaho, Hakusan, Ishikawa 924-0838, Japan. odas@neptune.kanazawa-it.ac.jp
authors.forename: Shinobu
authors.initials: S
authors.lastname: Oda
authors.forename: Hironori
authors.initials: H
authors.lastname: Wakui
authors.forename: Shinichi
authors.initials: S
authors.lastname: Ohashi
mesh_terms: D020095:Absidia / Q000201:enzymology*; D000085:Acetates / Q000378:metabolism*; D019149:Bioreactors; D002119:Calcium Carbonate; D004952:Esters; D006868:Hydrolysis; D008049:Lipase / Q000378:metabolism*; D008863:Microspheres
chemical_list: D000085:Acetates; D004952:Esters; C073401:2-ethylhexyl acetate; D008049:Lipase; D002119:Calcium Carbonate