title: [Analysis of porcine preadipocytes differentiation by atomic force microscope].
abstract: Abnormal changes during fat formation are closely related to the prevalence of many diseases. In order to understand the formation mechanism of fat, we used atomic force microscopy (AFM) to characterize the morphology and mechanical properties of porcine preadipocytes during the differentiation. Preadipocytes and adipocytes were different morphologically. The surface roughness of adipocytes was less than preadipocytes by detection of the ultrastructure. The mechanical properties of preadipocytes were changed during differentiation with AFM-based force spectroscopy. Preadipocytes were 20% higher than adipocytes in the adhesion force, stiffness and Young's modulus. Therefore, AFM analysis of membrane changes related to adipocytes formation provided quantitative data in the nanometer level for further studying the formation mechanism of the adipocytes.
authors.affiliation: Department of Chemistry, Jinan University, Guangzhou 510632, China.
authors.forename: Shengpu
authors.initials: S
authors.lastname: Li
authors.forename: Ruyi
authors.initials: R
authors.lastname: Shi
authors.forename: Qiulan
authors.initials: Q
authors.lastname: Wang
authors.forename: Mu
authors.initials: M
authors.lastname: Wang
authors.forename: Rui
authors.initials: R
authors.lastname: Gan
authors.forename: Jie
authors.initials: J
authors.lastname: Pan
authors.forename: Jiye
authors.initials: J
authors.lastname: Cai
authors.forename: Shouquan
authors.initials: S
authors.lastname: Zhang
mesh_terms: D017667:Adipocytes / Q000166:cytology* / Q000648:ultrastructure*; D050156:Adipogenesis; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D018625:Microscopy, Atomic Force; D013552:Swine