title: Study on chronic pancreatitis and pancreatic cancer using MRS and pancreatic juice samples.
abstract: OBJECTIVE
  To investigate the markers of pancreatic diseases and provide basic data and experimental methods for the diagnosis of pancreatic diseases.
  
  
  METHODS
  There were 15 patients in the present study, among whom 10 had pancreatic cancer and 5, chronic pancreatitis. In all patients, pancreatic cancer or chronic pancreatitis was located on the head of the pancreas. Pathology data of all patients was confirmed by biopsy and surgery. Among the 10 patients with pancreatic cancer, 3 people had a medical history of long-term alcohol consumption. Of 5 patients with chronic pancreatitis, 4 men suffered from alcoholic chronic pancreatitis. Pancreatic juice samples were obtained from patients by endoscopic retrograde cholangio-pancreatography. Magnetic resonance spectroscopyn was performed on an 11.7-T scanner (Bruker DRX-500) using Call-Purcell-Meiboom-Gill pulse sequences. The parameters were as follows: spectral width, 15 KHz; time domain, 64 K; number of scans, 512; and acquisition time, 2.128 s.
  
  
  RESULTS
  The main component of pancreatic juice included leucine, iso-leucine, valine, lactate, alanine, acetate, aspartate, lysine, glycine, threonine, tyrosine, histidine, tryptophan, and phenylalanine. On performing 1D (1)H and 2D total correlation spectroscopy, we found a triplet peak at the chemical shift of 1.19 ppm, which only appeared in the spectra of pancreatic juice obtained from patients with alcoholic chronic pancreatitis. This triplet peak was considered the resonance of the methyl of ethoxy group, which may be associated with the metabolism of alcohol in the pancreas.
  
  
  CONCLUSIONS
  The triplet peak, at the chemical shift of 1.19 ppm is likely to be the characteristic metabolite of alcoholic chronic pancreatitis.
authors.affiliation: Department of Radiology, Changhai Hospital, The Second Military Medical University, Shanghai 200433, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: Chao
authors.initials: C
authors.lastname: Ma
authors.forename: Zhuan
authors.initials: Z
authors.lastname: Liao
authors.forename: Bing
authors.initials: B
authors.lastname: Tian
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Lu
mesh_terms: D000368:Aged; D000596:Amino Acids / Q000032:analysis; D015415:Biomarkers / Q000032:analysis*; D005260:Female; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D008875:Middle Aged; D010189:Pancreatic Juice / Q000737:chemistry*; D010190:Pancreatic Neoplasms / Q000737:chemistry* / Q000503:physiopathology; D050500:Pancreatitis, Chronic / Q000378:metabolism* / Q000503:physiopathology
chemical_list: D000596:Amino Acids; D015415:Biomarkers