title: Enhanced immune response of T-cell independent or dependent antigens in SIGN-R1 knock-out mice.
abstract: Dextran was used to explore a novel method of enhancing an immune response against T-cell independent type 2 (TI-2) polysaccharide antigens, because of its suitability as a model for the immunogenecity of many TI-2 polysaccharide antigens and its high affinity to SIGN-R1. Here we showed that the primary immune response of IgM, IgG3, and IgG2b was enhanced by dextran in SIGN-R1 knock-out (KO) mice, further evoking the induction of a secondary immune response to IgG2b in parallel. On the other hand, an immune response of IgG1 and IgG2b against T-cell dependent (TD) antigen was strongly enhanced by the administration of ovalbumin (OVA) in SIGN-R1 KO mice. These results indicate that SIGN-R1 is critical in the regulation of immune responses. Therefore, our study suggests that inhibition of TI-2 polysaccharide antigen uptake in SIGN-R1(+) macrophages contributes to the development of novel vaccination strategies against TI-2 polysaccharide antigens.
authors.affiliation: Department of Biomedical Science and Technology, Institute of Biomedical Science and Technology, Konkuk University, Seoul, Republic of Korea.
authors.forename: Hyeong-Jwa
authors.initials: HJ
authors.lastname: Choi
authors.forename: Woo-Sung
authors.initials: WS
authors.lastname: Choi
authors.forename: Jin-Yeon
authors.initials: JY
authors.lastname: Park
authors.forename: Kyeong-Hyeon
authors.initials: KH
authors.lastname: Kang
authors.forename: Miglena G
authors.initials: MG
authors.lastname: Prabagar
authors.forename: Chan Young
authors.initials: CY
authors.lastname: Shin
authors.forename: Young-Sun
authors.initials: YS
authors.lastname: Kang
mesh_terms: D000818:Animals; D000955:Antigens, T-Independent / Q000276:immunology*; D001424:Bacterial Infections / Q000517:prevention & control; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D003911:Dextrans / Q000276:immunology / Q000494:pharmacology*; D005260:Female; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives* / Q000494:pharmacology; D056724:Immunity, Humoral / Q000187:drug effects*; D007074:Immunoglobulin G / Q000032:analysis / Q000276:immunology; D007075:Immunoglobulin M / Q000032:analysis / Q000276:immunology; D037181:Lectins, C-Type / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D008099:Liver / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010047:Ovalbumin / Q000276:immunology / Q000494:pharmacology; D011956:Receptors, Cell Surface / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D011971:Receptors, Immunologic / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014611:Vaccination / Q000379:methods; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
chemical_list: D000955:Antigens, T-Independent; D015815:Cell Adhesion Molecules; D003911:Dextrans; D016650:Fluorescein-5-isothiocyanate; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D037181:Lectins, C-Type; D010047:Ovalbumin; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin; C036109:dextran receptor; C015219:fluorescein isothiocyanate dextran