title: Identification of a phytase gene in barley (Hordeum vulgare L.).
abstract: BACKGROUND
  Endogenous phytase plays a crucial role in phytate degradation and is thus closely related to nutrient efficiency in barley products. The understanding of genetic information of phytase in barley can provide a useful tool for breeding new barley varieties with high phytase activity.
  
  
  RESULTS
  Quantitative trait loci (QTL) analysis for phytase activity was conducted using a doubled haploid population. Phytase protein was purified and identified by the LC-ESI MS/MS Shotgun method. Purple acid phosphatase (PAP) gene was sequenced and the position was compared with the QTL controlling phytase activity. A major QTL for phytase activity was mapped to chromosome 5 H in barley. The gene controlling phytase activity in the region was named as mqPhy. The gene HvPAP a was mapped to the same position as mqPhy, supporting the colinearity between HvPAP a and mqPhy.
  
  
  CONCLUSIONS
  It is the first report on QTLs for phytase activity and the results showed that HvPAP a, which shares a same position with the QTL, is a major phytase gene in barley grains.
authors.affiliation: Department of Agronomy, Zhejiang University, Hangzhou, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Dai
authors.forename: Long
authors.initials: L
authors.lastname: Qiu
authors.forename: Lingzhen
authors.initials: L
authors.lastname: Ye
authors.forename: Dezhi
authors.initials: D
authors.lastname: Wu
authors.forename: Meixue
authors.initials: M
authors.lastname: Zhou
authors.forename: Guoping
authors.initials: G
authors.lastname: Zhang
mesh_terms: D010832:6-Phytase / Q000235:genetics*; D002853:Chromatography, Liquid; D002874:Chromosome Mapping; D017343:Genes, Plant; D001467:Hordeum / Q000201:enzymology* / Q000235:genetics; D040641:Quantitative Trait Loci; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D010832:6-Phytase