title: Miniaturized enzyme production and development of micro-assays for cellulolytic and xylanolytic enzymes.
abstract: Miniaturized fungal cultivation and enzyme assays were developed. Cultivation for enzyme production was performed in 50mL conical tubes. In addition, the miniaturized enzyme assays reduced the amount of enzymes and reagents necessary. These procedures can be adopted in screening fungi to determine if they produce cellulolytic and xylanolytic enzymes.
authors.affiliation: Division of Environmental Science & Ecological Engineering, College of Life Sciences & Biotechnology, Korea University, 5-1 Anam-dong, Seongbuk-gu, Seoul, 136-701 Republic of Korea.
authors.forename: Young Min
authors.initials: YM
authors.lastname: Lee
authors.forename: Hanbyul
authors.initials: H
authors.lastname: Lee
authors.forename: Gyu-Hyeok
authors.initials: GH
authors.lastname: Kim
authors.forename: Jae-Jin
authors.initials: JJ
authors.lastname: Kim
mesh_terms: D002480:Cellulase / Q000032:analysis* / Q000378:metabolism; D057075:Enzyme Assays / Q000379:methods*; D005656:Fungal Proteins / Q000032:analysis* / Q000378:metabolism; D005658:Fungi / Q000737:chemistry / Q000201:enzymology*; D014995:Xylosidases / Q000032:analysis* / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D014995:Xylosidases; D002480:Cellulase