title: Different ischemic preconditioning regimens affecting preservation injury of intestines.
abstract: Decreasing ischemia-reperfusion injury in intestinal transplantation is of paramount importance for improving graft recovery and function. This study explores the ability of two ischemic preconditioning (IPC) regimens to reduce preservation injury. Sprague-Dawley rats were divided into 3 groups (n = 11 each). In the controls (group C), intestinal grafts were harvested and preserved. IPC was performed either through 4 cycles of mesenteric ischemia of 4 min each followed by 10 min of reperfusion (group BIPC) or 2 ischemic cycles of 12 min each followed by 10 min of reperfusion (group LIPC). Grafts were stored in histidine-tryptophan-ketoglutarate, and samples were taken 0, 3, 6, 9, 12, 18, and 24 h after preservation. Preservation injury was scored using the Park/Chiu scale. Goblet cells (GC), enteroendocrine cells (EEC) and serotonin-producing EEC (SPEEC) were studied for evaluation of the graft conditions. Group C had the most advanced preservation injury followed by group BIPC. GC count was lowest in group C, followed by BIPC. Comparison between groups BIPC and LIPC showed superior parameters (preservation injury, GC, EEC, and SPEEC) in LIPC. In conclusion, an IPC regimen of 2 ischemic cycles of 12 min each followed by 10 min of reperfusion distinctly decreased the preservation injury of intestinal grafts compared with non-manipulated grafts.
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, P.J. Šafárik University, Košice, Slovakia. janko.varga@gmail.com
authors.forename: Ján
authors.initials: J
authors.lastname: Varga
authors.forename: Stefan
authors.initials: S
authors.lastname: Tóth
authors.forename: Pavel
authors.initials: P
authors.lastname: Staško
authors.forename: Martin
authors.initials: M
authors.lastname: Bujdoš
authors.forename: Jarmila
authors.initials: J
authors.lastname: Veselá
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Jonecová
authors.forename: Mikuláš
authors.initials: M
authors.lastname: Pomfy
mesh_terms: D000818:Animals; D002452:Cell Count; D019858:Enteroendocrine Cells / Q000166:cytology; D020397:Goblet Cells / Q000166:cytology; D007422:Intestines / Q000098:blood supply* / Q000473:pathology / Q000637:transplantation*; D019194:Ischemic Preconditioning; D008297:Male; D009926:Organ Preservation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000473:pathology / Q000517:prevention & control*; D012701:Serotonin / Q000096:biosynthesis
chemical_list: D012701:Serotonin