title: The composition of upstream open reading frames (uORF) in four genes from Trypanosoma cruzi typical strains.
abstract: Upstream open reading frames (uORF) are small open reading frames located in the 5' untranslated region (5' utr) of a mature mRNA. We analysed in four strains representing the Trypanosoma cruzi groups Tc I, Tc II, Tc IV and Tc VI the uORF present in 5' utr sequences of four genes: P-type H+-ATPase 1, DEAD/H RNA helicase, casein kinase 1.1 and ferredoxin-NADP+ reductase. A segment in the 5' utr at each of these genes encompassing one or more uORF was PCR amplified and sequenced. An analysis of these sequences reveals that the uORF in T. cruzi show minor variations; however, these nucleotide substitutions mirror the divergence of T. cruzi strains into major groups.
authors.affiliation: Laboratory of Interdisciplinary Medical Researches, Instituto Oswaldo Cruz, Fiocruz Av. Brasil 4365, 21040-360 Rio de Janeiro, Brazil.
authors.forename: Lauren Hubert
authors.initials: LH
authors.lastname: Jaeger
authors.forename: Adeilton
authors.initials: A
authors.lastname: Brandão
mesh_terms: D020121:5' Untranslated Regions; D001483:Base Sequence; D047388:Casein Kinases / Q000737:chemistry / Q000235:genetics; D014355:Chagas Disease / Q000469:parasitology*; D005287:Ferredoxin-NADP Reductase / Q000737:chemistry / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D006180:Proton-Translocating ATPases / Q000737:chemistry / Q000235:genetics; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics*; D020365:RNA Helicases / Q000737:chemistry / Q000235:genetics; D014158:Transcription, Genetic; D014349:Trypanosoma cruzi / Q000145:classification / Q000201:enzymology / Q000235:genetics*
chemical_list: D020121:5' Untranslated Regions; D015800:Protozoan Proteins; D005287:Ferredoxin-NADP Reductase; D047388:Casein Kinases; D006180:Proton-Translocating ATPases; D020365:RNA Helicases