title: Binding of the cell adhesive protein tropoelastin to PTFE through plasma immersion ion implantation treatment.
abstract: The interaction of proteins and cells with polymers is critical to their use in scientific and medical applications. In this study, plasma immersion ion implantation (PIII) was used to modify the surface of polytetrafluorethylene (PTFE), enabling the covalent binding of a cell adhesive protein, tropoelastin, without employing chemical linking molecules. Tropoelastin coating of untreated or PIII treated PFTE simultaneously promoted and blocked cell interactions respectively, i.e. PIII treatment of the PTFE surface completely inverses the cell interactive properties of bound tropoelastin. This activity persisted over long term storage of the PIII treated surfaces. The integrin binding C-terminus of tropoelastin was markedly less solvent exposed when bound to PIII treated PTFE than untreated PTFE, accounting for the modulation of cell adhesive activity. This presents a new methodology to specifically modulate cell behavior on a polymer surface using a simple one step treatment process, by adjusting the adhesive activity of a single extracellular matrix protein.
authors.affiliation: Applied and Plasma Physics, School of Physics, University of Sydney, Building A28, Sydney, NSW 2006, Australia. daniel.bax@sydney.edu.au
authors.forename: Daniel V
authors.initials: DV
authors.lastname: Bax
authors.forename: Yiwei
authors.initials: Y
authors.lastname: Wang
authors.forename: Zhe
authors.initials: Z
authors.lastname: Li
authors.forename: Peter K M
authors.initials: PK
authors.lastname: Maitz
authors.forename: David R
authors.initials: DR
authors.lastname: McKenzie
authors.forename: Marcela M M
authors.initials: MM
authors.lastname: Bilek
authors.forename: Anthony S
authors.initials: AS
authors.lastname: Weiss
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology; D006801:Humans; D007477:Ions / Q000737:chemistry*; D008297:Male; D008422:Materials Testing; D051379:Mice; D008807:Mice, Inbred BALB C; D011138:Polytetrafluoroethylene / Q000737:chemistry* / Q000378:metabolism*; D011485:Protein Binding; D013499:Surface Properties; D014333:Tropoelastin / Q000737:chemistry / Q000378:metabolism*
chemical_list: D020099:Coated Materials, Biocompatible; D007477:Ions; D014333:Tropoelastin; D011138:Polytetrafluoroethylene