title: Transcription initiation from within P elements generates hypomorphic mutations in Drosophila melanogaster.
abstract: Numerous transposable element insertions in Drosophila melanogaster cause hypomorphic mutations. We report that transcription initiation within a region found in many P-element constructs provides an explanation for why some gene function is retained. We detected evidence of this transcription in four different types of P constructs, regardless of whether the insertion was in a coding exon, intron, 5' untranslated region, or upstream of the gene span.
authors.affiliation: Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, North Carolina 27599, USA.
authors.forename: Matthew C
authors.initials: MC
authors.lastname: LaFave
authors.forename: Jeff
authors.initials: J
authors.lastname: Sekelsky
mesh_terms: D020121:5' Untranslated Regions; D000483:Alleles; D000818:Animals; D001483:Base Sequence; D004251:DNA Transposable Elements; D029721:Drosophila Proteins / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics*; D005091:Exons; D005260:Female; D015870:Gene Expression; D017344:Genes, Insect; D007438:Introns; D007814:Larva / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009154:Mutation; D010641:Phenotype; D011679:Pupa / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D020121:5' Untranslated Regions; D004251:DNA Transposable Elements; D029721:Drosophila Proteins; D012333:RNA, Messenger