title: Tissue-engineered bone formation using periosteal-derived cells and polydioxanone/pluronic F127 scaffold with pre-seeded adipose tissue-derived CD146 positive endothelial-like cells.
abstract: The aim of this study was to generate tissue-engineered bone formation using periosteal-derived cells seeded into a polydioxanone/pluronic F127 (PDO/Pluronic F127) scaffold with adipose tissue-derived CD146 positive endothelial-like cells. Considering the hematopoietic and mesenchymal phenotypes of adipose tissue-derived cells cultured in EBM-2 medium, CD146 positive adipose tissue-derived cells was sorted to purify more endothelial cells in characterization. These sorted cells were referred to as adipose tissue-derived CD146 positive endothelial-like cells. Periosteum is a good source of osteogenic cells for tissue-engineered bone formation. Periosteal-derived cells were found to have good osteogenic capacity in a PDO/Pluronic F127 scaffold, which could provide a suitable environment for the osteoblastic differentiation of these cells. Through the investigation of capillary-like tube formation on matrigel and the cellular proliferation of adipose tissue-derived CD146 positive endothelial-like cells cultured in different media conditions, we examined these cells could be cultured in EBM-2 with osteogenic induction factors. We also observed that the osteogenic activity of periosteal-derived cells could be good in EBM-2 with osteogenic induction factors, in the early period of culture. The experimental results obtained in the miniature pig model suggest that tissue-engineered bone formation using periosteal-derived cells and PDO/Pluronic F127 scaffold with pre-seeded adipose tissue-derived CD146 positive endothelial-like cells can be used to restore the bony defects of the maxillofacial region when used in clinics.
authors.affiliation: Department of Advanced Materials, College of Life Science and Nano Technology, Hannam University, 133 Ojeong-dong, Daedeog gu, Daejeon 306-791, South Korea.
authors.forename: Jin-Ho
authors.initials: JH
authors.lastname: Lee
authors.forename: Jin-Hyun
authors.initials: JH
authors.lastname: Kim
authors.forename: Se-Heang
authors.initials: SH
authors.lastname: Oh
authors.forename: Sang-June
authors.initials: SJ
authors.lastname: Kim
authors.forename: Young-Sool
authors.initials: YS
authors.lastname: Hah
authors.forename: Bong-Wook
authors.initials: BW
authors.lastname: Park
authors.forename: Deok Ryong
authors.initials: DR
authors.lastname: Kim
authors.forename: Gyu-Jin
authors.initials: GJ
authors.lastname: Rho
authors.forename: Geun-Ho
authors.initials: GH
authors.lastname: Maeng
authors.forename: Ryoung-Hoon
authors.initials: RH
authors.lastname: Jeon
authors.forename: Hee-Chun
authors.initials: HC
authors.lastname: Lee
authors.forename: Jong-Ryoul
authors.initials: JR
authors.lastname: Kim
authors.forename: Gyoo-Cheon
authors.initials: GC
authors.lastname: Kim
authors.forename: Uk-Kyu
authors.initials: UK
authors.lastname: Kim
authors.forename: June-Ho
authors.initials: JH
authors.lastname: Byun
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000293:Adolescent; D051929:CD146 Antigen / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism; D006801:Humans; D008422:Materials Testing; D010012:Osteogenesis / Q000502:physiology*; D010521:Periosteum / Q000166:cytology*; D020442:Poloxamer / Q000737:chemistry*; D016687:Polydioxanone / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D051929:CD146 Antigen; D003470:Culture Media; D013501:Surface-Active Agents; D020442:Poloxamer; D016687:Polydioxanone