title: Cationic poly(amidoamine) dendrimers as additives for capillary electroseparation and detection of proteins.
abstract: We examine the influence of cationic poly(amidoamine) (PAMAM) dendrimers on capillary electroseparation-UV analysis of proteins. PAMAMs adsorbing to the capillary surface suppressed the wall-adsorption of proteins; meanwhile, PAMAMs added to the buffer exhibited selectivity toward proteins. Presence of 3 x 10⁻⁴  g/mL PAMAM generation one (G 1.0) in 30 mM phosphate, at pH 2.6, rendered significant enhancement in separation efficiency; the merged peaks of myoglobin and trypsin inhibitor were separated. Moreover, the protein-dendrimer interactions changed the inherent UV absorbance profiles of proteins. UV-Vis study showed that the absorbance of cytochrome C and transferrin increased at the detection wavelength of 214 nm; their detection sensitivity enhanced by 2.44 and 2.01-folds, respectively, with addition of 5 x 10⁻⁴  g/mL PAMAM G 1.0.
authors.affiliation: College of Chemistry, Beijing Normal University, Beijing, 100875, P. R. China.
authors.forename: Qingchun
authors.initials: Q
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Tian
authors.forename: Chenling
authors.initials: C
authors.lastname: Zhang
authors.forename: Huan
authors.initials: H
authors.lastname: Yang
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Weidong
authors.initials: W
authors.lastname: Qin
authors.forename: Zhengping
authors.initials: Z
authors.lastname: Liu
mesh_terms: D000818:Animals; D002412:Cations / Q000737:chemistry; D002417:Cattle; D050091:Dendrimers / Q000737:chemistry*; D004573:Electrolytes; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D010710:Phosphates / Q000737:chemistry; D010756:Phosphoric Acids / Q000737:chemistry; D011506:Proteins / Q000302:isolation & purification*
chemical_list: D002412:Cations; D050091:Dendrimers; D004573:Electrolytes; C104700:PAMAM Starburst; D010710:Phosphates; D010756:Phosphoric Acids; D011506:Proteins; C030242:phosphoric acid; C018279:sodium phosphate