title: Long-term nano-mechanical properties of biomodified dentin-resin interface components.
abstract: Failures of dental composite restorative procedures are largely attributed to the degradation of dentin-resin interface components. Biomodification of dentin using bioactive agents may improve the quality and durability of the dentin-resin bonds. The aim of this study was to nanomechanically assess the reduced modulus of elasticity (Er) and nano-hardness (H) of major components of the dentin-resin interface (hybrid layer, adhesive layer and underlying dentin) biomodified by collagen cross-linkers at 24h, 3 and 6 months following restorative procedure. Demineralized dentin surfaces were biomodified with 5% glutaraldehyde (GD) or 6.5% grape seed extract (GSE) prior to placement of adhesive systems and composite resin. Nano-measurements of the interface components in a fluid cell showed that both agents increased the Er and H of underlying dentin after 3 and 6 months when compared to a control. The mechanical properties of the adhesive and hybrid layers decreased over time. Biomodification of the dentin-resin interface structures using GD and GSE can increase the mechanical properties of the interface over time and may contribute to the long-term quality of adhesive restorations.
authors.affiliation: Department of Dental Materials and Prosthodontics, Araçatuba School of Dentistry, UNESP, Araçatuba, SP, Brazil.
authors.forename: Paulo Henrique
authors.initials: PH
authors.lastname: Dos Santos
authors.forename: Sachin
authors.initials: S
authors.lastname: Karol
authors.forename: Ana Karina
authors.initials: AK
authors.lastname: Bedran-Russo
mesh_terms: D000268:Adhesiveness; D001672:Biocompatible Materials / Q000737:chemistry*; D001696:Biomechanical Phenomena; D003094:Collagen / Q000737:chemistry; D003804:Dentin / Q000737:chemistry*; D004548:Elasticity; D005976:Glutaral / Q000737:chemistry; D006801:Humans; D008422:Materials Testing; D036103:Nanotechnology / Q000379:methods*; D044945:Proanthocyanidins / Q000737:chemistry; D012117:Resins, Synthetic / Q000737:chemistry; D013499:Surface Properties; D013718:Tensile Strength; D013997:Time Factors
chemical_list: D001672:Biocompatible Materials; D044945:Proanthocyanidins; D012117:Resins, Synthetic; C013221:proanthocyanidin; D003094:Collagen; D005976:Glutaral