title: Characterization of intracellular translocation of Forkhead transcription factor O (FoxO) members induced by NGF in PC12 cells.
abstract: Nuclear translocation of Forkhead transcription factors of the O class (FoxOs) is important for the action of growth factors. However it is not known if all members of the FOXO family have the same translocation properties. We examined the effects of nerve growth factor (NGF) on nuclear/cytoplasmic shuttling of FoxO1, FoxO3a and FoxO6 in PC12 cells and determined their translocation kinetics. Our data demonstrated that NGF could induce the nuclear exclusion of FoxO1-GFP and FoxO3a-GFP in PC12 cells with different properties, but had no effect on FoxO6-GFP's nuclear localization and FoxO6-GFP showed an exclusive nuclear localization. Translocat ould be blocked by K252a and LY294002 but not by PD98059. Moreover, FoxO3a returned to cytoplasm at a higher rate than FoxO1 after NGF stimulation and it was more sensitive than FoxO1 to NGF stimulation.
authors.affiliation: Neuropharmacology, School of Pharmaceutical Science, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wen
authors.forename: Xiaolu
authors.initials: X
authors.lastname: Duan
authors.forename: Rifang
authors.initials: R
authors.lastname: Liao
authors.forename: Peter
authors.initials: P
authors.lastname: Little
authors.forename: Guoquan
authors.initials: G
authors.lastname: Gao
authors.forename: Hao
authors.initials: H
authors.lastname: Jiang
authors.forename: Srivastava
authors.initials: S
authors.lastname: Lalit
authors.forename: Remi
authors.initials: R
authors.lastname: Quirion
authors.forename: Wenhua
authors.initials: W
authors.lastname: Zheng
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002467:Cell Nucleus / Q000737:chemistry / Q000378:metabolism; D003593:Cytoplasm / Q000737:chemistry / Q000378:metabolism; D000071316:Forkhead Box Protein O3; D051858:Forkhead Transcription Factors / Q000378:metabolism*; D042541:Intracellular Space / Q000378:metabolism; D020932:Nerve Growth Factor / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism; D016716:PC12 Cells; D021381:Protein Transport / Q000502:physiology*; D051381:Rats; D000071161:Forkhead Box Protein O1
chemical_list: D000071316:Forkhead Box Protein O3; D051858:Forkhead Transcription Factors; D020932:Nerve Growth Factor; D009419:Nerve Tissue Proteins; C499515:FOXO3 protein, rat; C562177:Foxo6 protein, rat; C074639:Foxo1 protein, rat; D000071161:Forkhead Box Protein O1