title: [Effects of phytohormones on plant regeneration and production of flavonoids in transgenic Saussurea involucrata hairy roots].
abstract: We investigated the plant regeneration and production of flavonoids in three high-yield flavonoids transgenic Saussurea involucrata hairy roots C17, C27 and C46 by quantification of two phytohormones GA3 and IAA. The results showed that GA3 concentration at more than 1.0 mg/L could induce adventitious shoots in the hairy root lines. The highest shoot regeneration rate, about 82%, was obtained when the hairy roots C17 were cultured with 2.0 mg/L GA3. The results on HPLC and UV spectrophotometry showed that exogenous application of both GA3 and IAA increased the content of flavonoids in the hairy roots. The contents of flavonoids and apigenin in the hormone-treated hairy roots and regenerates were higher comparing with those in the untreated hairy roots and the regenerates. However, the content of flavonoids was not related to tissue weight, and was negatively related to the regeneration efficiency.
authors.affiliation: Key Laboratory for Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
authors.forename: Xianli
authors.initials: X
authors.lastname: Qiao
authors.forename: Shuguang
authors.initials: S
authors.lastname: Jiang
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Li
authors.forename: Fengxia
authors.initials: F
authors.lastname: Li
authors.forename: Dexiu
authors.initials: D
authors.lastname: Zhao
mesh_terms: D046508:Culture Techniques; D005419:Flavonoids / Q000096:biosynthesis*; D005875:Gibberellins / Q000494:pharmacology; D007210:Indoleacetic Acids / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000254:growth & development / Q000378:metabolism*; D030441:Saussurea / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism
chemical_list: D005419:Flavonoids; D005875:Gibberellins; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; C030737:indoleacetic acid; C007842:gibberellic acid