title: Hesperidin, a flavone glycoside, as mediator of neuronal survival.
abstract: Flavonoids comprise the most common group of plant polyphenols and provide much of the flavor and color to fruits and vegetables. More than 5,000 different flavonoids have been described. The biological activities of flavonoids cover a very broad spectrum, from anticancer and antibacterial activities to inhibition of bone reabsorption and neuroprotection effect. Although emerging evidence suggests that flavonoids have an important role on brain development, little is known about their mechanisms of action. In the present work, we performed a screening of flavonoid actions by analyzing the effects of these substances (hesperidin and rutin) on neural progenitors and neuronal morphogenesis in vitro. We demonstrated that treatment of neural progenitors with the flavonoid hesperidin enhanced neuronal population as revealed by an 80% increase in the number of β-tubulin III cells. This effect was mainly due to modulation of neuronal progenitor survival. Pools of astrocyte and oligodendrocyte progenitors were not affected by hesperidin whereas rutin had no effect on neuronal population. We also demonstrated that the flavonoid hesperidin modulates neuronal cell death by activating MAPK and PI3K pathways. This opens the possibility of using flavonoids for potential new therapeutic strategies for neurodegenerative diseases.
authors.affiliation: Laboratório de Neurobiologia Celular, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde, Bloco F, Sala F15. Ilha do Fundão, 21941-590 Rio de Janeiro, Brazil.
authors.forename: Jader
authors.initials: J
authors.lastname: Nones
authors.forename: Tania Cristina Leite de Sampaio
authors.initials: TC
authors.lastname: E Spohr
authors.forename: Flávia Carvalho Alcantara
authors.initials: FC
authors.lastname: Gomes
mesh_terms: D000818:Animals; D016923:Cell Death / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D006569:Hesperidin / Q000494:pharmacology*; D006801:Humans; D051379:Mice; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D058953:Neural Stem Cells / Q000166:cytology / Q000187:drug effects*; D009474:Neurons / Q000166:cytology / Q000187:drug effects*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry; D012431:Rutin / Q000494:pharmacology; D015398:Signal Transduction / Q000502:physiology; D014404:Tubulin / Q000378:metabolism
chemical_list: D010936:Plant Extracts; D014404:Tubulin; D012431:Rutin; D006569:Hesperidin; D019869:Phosphatidylinositol 3-Kinases; D020928:Mitogen-Activated Protein Kinases