title: The need for enzymatic steering in abietic acid biosynthesis: gas-phase chemical dynamics simulations of carbocation rearrangements on a bifurcating potential energy surface.
abstract: Abietic acid, a constituent of pine resin, is naturally derived from abietadiene --a process that requires four enzymes: one (abietadiene synthase) for conversion of the acyclic, achiral geranylgeranyl diphosphate to the polycyclic, chiral abietadiene (a complex process involving the copalyl diphosphate intermediate) and then three to oxidize a single methyl group of abietadiene to the corresponding carboxylic acid. In previous work (Nature Chem.2009, 1, 384), electronic structure calculations on carbocation rearrangements leading to abietadienyl cation revealed an interesting potential energy surface with a bifurcating reaction pathway (two transition-state structures connected directly with no intervening minimum), which links two products--one natural and one not yet isolated from Nature. Herein we describe direct dynamics simulations of the key step in the formation of abietadiene (in the gas phase and in the absence of the enzyme). The simulations reveal that abietadiene synthase must intervene in order to produce abietadiene selectively, in essence steering this reaction to avoid the generation of byproducts with different molecular architectures.
authors.affiliation: Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, Texas 79409, USA.
authors.forename: Matthew R
authors.initials: MR
authors.lastname: Siebert
authors.forename: Jiaxu
authors.initials: J
authors.lastname: Zhang
authors.forename: Srirangam V
authors.initials: SV
authors.lastname: Addepalli
authors.forename: Dean J
authors.initials: DJ
authors.lastname: Tantillo
authors.forename: William L
authors.initials: WL
authors.lastname: Hase
mesh_terms: D045784:Abietanes / Q000096:biosynthesis* / Q000378:metabolism*; D007535:Isomerases / Q000378:metabolism; D008956:Models, Chemical; D056004:Molecular Dynamics Simulation; D010755:Organophosphates / Q000378:metabolism; D012116:Resins, Plant / Q000378:metabolism
chemical_list: D045784:Abietanes; D010755:Organophosphates; D012116:Resins, Plant; C101761:abieta-7(8),13(14)-diene; C408747:copalyl diphosphate; C013893:rosin; D007535:Isomerases; C088741:abietadiene cyclase; C023710:abietic acid