title: The Golgi protein p115 associates with gamma-tubulin and plays a role in Golgi structure and mitosis progression.
abstract: The Golgi apparatus is a network of polarized cisternae localized to the perinuclear region in mammalian cells. It undergoes extensive vesiculation at the onset of mitosis and its reassembly requires factors that are in part segregated via the mitotic spindle. Here we show that unlike typical Golgi markers, the Golgi-protein p115 partitioned with the spindle poles throughout mitosis. An armadillo-fold in its N terminus mediated a novel interaction between p115 and γ-tubulin and functioned in its centrosomal targeting. Both the N- and C-terminal regions of p115 were required to maintain Golgi structure. Strikingly, p115 was essential for mitotic spindle function and the resolution of the cytokinetic bridge because its depletion resulted in spindle collapse, chromosome missegregation, and failed cytokinesis. We demonstrate that p115 plays a critical role in mitosis progression, implicating it as the only known golgin to regulate both mitosis and apoptosis.
authors.affiliation: Department of Developmental and Molecular Biology, Albert Einstein College of Medicine of Yeshiva University, Bronx, New York 10461, USA. aradulescu@ucsd.edu
authors.forename: Andreea E
authors.initials: AE
authors.lastname: Radulescu
authors.forename: Shaeri
authors.initials: S
authors.lastname: Mukherjee
authors.forename: Dennis
authors.initials: D
authors.lastname: Shields
mesh_terms: D000818:Animals; D017209:Apoptosis; D019556:COS Cells; D045744:Cell Line, Tumor; D002522:Chlorocebus aethiops; D002875:Chromosomes / Q000648:ultrastructure; D005786:Gene Expression Regulation; D006056:Golgi Apparatus / Q000378:metabolism*; D000077614:Golgi Matrix Proteins; D006367:HeLa Cells; D006801:Humans; D008938:Mitosis; D017434:Protein Structure, Tertiary; D008941:Spindle Apparatus / Q000378:metabolism; D014404:Tubulin / Q000378:metabolism*; D033921:Vesicular Transport Proteins / Q000378:metabolism / Q000502:physiology*
chemical_list: D000077614:Golgi Matrix Proteins; D014404:Tubulin; D033921:Vesicular Transport Proteins; C091580:vesicular transport factor p115