title: Identification of biotic and abiotic stress up-regulated ESTs in Gossypium arboreum.
abstract: Asiatic desi cotton (Gossypium arboreum) shows great potential against biotic and abiotic stresses. The stress resistant nature makes it a best source for the identification of biotic and abiotic stress resistant genes. As in many plants same set of genes show responding behavior against the various abiotic and biotic stresses. Thus in the present study the ESTs from the G. arboreum drought stressed leaves were subjected to find the up-regulated ESTs in abiotic and biotic stresses through homology and in-silico analysis. A cDNA library has been constructed from the drought stressed G. arboreum plant. 778 clones were randomly picked and sequenced. All these sequences were subjected to in-silico identification of biotic and abiotic up-regulated ESTs. Total 39 abiotic and biotic up-regulated ESTs were identified. The results were further validated by real-time PCR; by randomly selection of ten ESTs. These findings will help to develop stress resistant crop varieties for better yield and growth performance under stresses.
authors.affiliation: Department of Botany, University of Baluchistan, Sariab Road, Quetta, Pakistan. barozaikhan@gmail.com
authors.forename: Muhammad Younas Khan
authors.initials: MY
authors.lastname: Barozai
authors.forename: Tayyab
authors.initials: T
authors.lastname: Husnain
mesh_terms: D001483:Base Sequence; D001947:Breeding / Q000379:methods; D055864:Droughts; D020224:Expressed Sequence Tags / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology*; D015723:Gene Library; D003368:Gossypium / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013312:Stress, Physiological / Q000502:physiology*