title: Waveguide evanescent field fluorescence microscopy: high contrast imaging of a domain forming mixed lipid Langmuir-Blodgett monolayer mimicking lung surfactant.
abstract: Waveguide evanescent field fluorescence (WEFF) microscopy is a new development that allows the imaging of contact regions between biological cells and their substratum, as well as imaging of ultrathin films such as monomolecular Langmuir-Blodgett (LB) films. Mixed-lipid monolayer systems mimicking lung surfactant were fabricated on waveguides using the LB technique and imaged by both WEFF and standard wide field epi-fluorescence microscopy. These two technologies were compared with respect to contrast, photobleaching, and sensitivity. It was found that WEFF microscopy produced images with a much higher contrast, lower photobleaching, and higher sensitivity. In addition, fine structures in the lipidic domains were observed for the first time.
authors.affiliation: Universityof Kurdistan, Department of Physics, Sanandej, Kurdistan, Iran.
authors.forename: Abdollah
authors.initials: A
authors.lastname: Hassanzadeh
authors.forename: Silvia
authors.initials: S
authors.lastname: Mittler
mesh_terms: D020404:Glycerophospholipids / Q000737:chemistry*; D008567:Membranes, Artificial; D008856:Microscopy, Fluorescence / Q000379:methods*; D008956:Models, Chemical; D019308:Palmitic Acid / Q000737:chemistry*; D038761:Photobleaching; D011312:Pressure; D011663:Pulmonary Surfactants / Q000737:chemistry*; D012680:Sensitivity and Specificity
chemical_list: D020404:Glycerophospholipids; D008567:Membranes, Artificial; D011663:Pulmonary Surfactants; D019308:Palmitic Acid