title: Image cytometry characterization of ploidy level, proliferative activity and chromatin pattern in 50 nasal polyps.
abstract: A computer-assisted microscope analysis of Feulgen-stained nuclei was carried out on a series of 50 nasal polyps in order to try to identify specific biological subgroups. The present series of 50 nasal polyps includes single polyps both associated (n=9) and unassociated (n=9) with allergy and diffuse polyposis both associated (n=7) and unassociated (n=9) with allergy, cystic fibrosis (n=9) and ASA (aspirin-sinusitis-asthma) related polyposis (n=7). The computer-assisted microscope analysis provides 36 quantitative variables which include 1 variable describing proliferative activity, 9 describing the nuclear desoxyribonucleic acid distribution (DNA ploidy level) and 26 describing nucleus morphology, i.e. its size and chromatin pattern. The results show that the methodology proposed here enabled four major groups of nasal polyps to be identified, i.e. diffuse polyposis associated with allergy, cystic fibrosis-related polyposis, single polyps both associated and unassociated either with allergy and a fourth group including diffuse polyposis not associated with allergy and ASA-related polyposis. These four groups of nasal polyps differed markedly in their morphonuclear characteristics, but not in the proliferative activity- and DNA ploidy-related variables.
authors.affiliation: FREE UNIV BRUSSELS,FAC MED,HISTOL LAB,B-1070 BRUSSELS,BELGIUM. FREE UNIV BRUSSELS,ERASME ACAD HOSP,ENT DEPT,BRUSSELS,BELGIUM. FREE UNIV BRUSSELS,ERASME ACAD HOSP,DEPT PATHOL,BRUSSELS,BELGIUM. FREE UNIV BRUSSELS,INST INTERDISCIPLINARY RES & DEV ARTIFICIAL INTEL,BRUSSELS,BELGIUM.
authors.forename: S
authors.initials: S
authors.lastname: Hassid
authors.forename: G
authors.initials: G
authors.lastname: Choufani
authors.forename: O
authors.initials: O
authors.lastname: Elkattabi
authors.forename: S
authors.initials: S
authors.lastname: Dawance
authors.forename: C
authors.initials: C
authors.lastname: Decaestecker
authors.forename: M
authors.initials: M
authors.lastname: Brugmans
authors.forename: A
authors.initials: A
authors.lastname: Danguy
authors.forename: J
authors.initials: J
authors.lastname: Pasteels
authors.forename: I
authors.initials: I
authors.lastname: Salmon
authors.forename: R
authors.initials: R
authors.lastname: Kiss