title: Establishment of a continuous cell line from a human carcinoid of the small intestine (KRJ-I).
abstract: A new continuous cell line from a human malignant carcinoid of the small intestine (KRJ-I) was established. The cells showed morphological and immunocytochemical features of the tumor of origin and expressed estrogen receptors. The cells are growing as a suspension, forming multicellular aggregates and spheroids. Electron microscopy confirmed the presence of neuroendocrine granules. Dose-dependent growth inhibition was observed after incubation with 5-azacytidine. Cytogenetic analyses of the tumor of origin, the cell line KRJ-I and a liver metastasis KRJ-II revealed clonal tetraploidy and clonal loss of the Y-chromosome and chromosome 19.
authors.affiliation: GRAZ UNIV,SCH MED,DEPT INTERNAL MED,A-8010 GRAZ,AUSTRIA. GRAZ UNIV,SCH MED,DEPT MED BIOL & HUMAN GENET,A-8010 GRAZ,AUSTRIA. UNIV VIENNA,SCH MED,DEPT SURG,A-1010 VIENNA,AUSTRIA. UNIV VIENNA,RES INST LAB ANIM BREEDING,A-1010 VIENNA,AUSTRIA. RES INST ELECTRON MICROSCOPY & FINE STRUCT,GRAZ,AUSTRIA. GRAZ UNIV TECHNOL,A-8010 GRAZ,AUSTRIA.
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authors.initials: R
authors.lastname: Pfragner
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authors.lastname: Wirnsberger
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authors.lastname: Niederle
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authors.initials: A
authors.lastname: Behmel
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authors.lastname: Rinner
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authors.lastname: Mandl
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authors.lastname: Wawrina
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authors.lastname: Luo
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authors.lastname: Ingolic
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authors.lastname: Schauenstein