title: Evaluation of ABC efflux transporters genes expression in kidney of rainbow trout (Oncorhynchus mykiss) fed with melamine and cyanuric acid diets.
abstract: Gene expression experiments were targeted in order to monitor the ABC efflux transporters, which is potentially involved in cellular detoxification/defense. Changes in expression levels of different ABC genes in kidney of Oncorhynchus mykiss fed with melamine and melamine+cyanuric acid enriched diets were recorded in both treated groups by mRNA ΔΔ(CT) relative quantification method. Expression profiles of eight different ABC genes basically showed low alterations in melamine group and more consistent changes in melamine+cyanuric acid treated fish, compared with own control. In the last group ABCC2 gene over expression was the more evident alteration. These results suggest that ABC efflux system could be involved in mobilization of hydrophilic molecules in the forcing condition of chronic exposure.
authors.affiliation: CReAA-National Reference Center for the Surveillance and Monitoring of Animal Feed, Istituto Zooprofilattico Sperimentale del Piemonte, Turin, Italy. alessandro.benedetto@izsto.it
authors.forename: Alessandro
authors.initials: A
authors.lastname: Benedetto
authors.forename: Stefania
authors.initials: S
authors.lastname: Squadrone
authors.forename: Marino
authors.initials: M
authors.lastname: Prearo
authors.forename: Antonia Concetta
authors.initials: AC
authors.lastname: Elia
authors.forename: Ilaria
authors.initials: I
authors.lastname: Giorgi
authors.forename: Maria Cesarina
authors.initials: MC
authors.lastname: Abete
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D004032:Diet; D015870:Gene Expression / Q000187:drug effects; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D000089762:Multidrug Resistance-Associated Protein 2; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000235:genetics / Q000378:metabolism; D017686:Oncorhynchus mykiss / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D014227:Triazines / Q000008:administration & dosage / Q000378:metabolism / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000008:administration & dosage / Q000378:metabolism / Q000633:toxicity*
chemical_list: D018528:ATP-Binding Cassette Transporters; D000089762:Multidrug Resistance-Associated Protein 2; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D012333:RNA, Messenger; D014227:Triazines; D014874:Water Pollutants, Chemical; C004632:cyanuric acid; C011907:melamine