title: Differential growth-related gene expression in abalone (Haliotis midae).
abstract: The slow growth rate of Haliotis midae impedes the optimal commercial production of this most profitable South African aquaculture species. To date, no comprehensive effort has been made to identify genes associated with growth variation in farmed H. midae. The aim of this study was therefore to investigate growth variation in H. midae and to identify and quantify the expression of selected growth-related genes. Towards this aim, molecular methodologies and cell cultures were combined as a time-efficient and economical way of studying abalone transcriptomics and cell biology. Modern Illumina sequencing-by-synthesis technology and subsequent sequence annotation were used to elucidate differential gene expression between two sibling groups of abalone demonstrating significant growth variation. The expression of selected target genes involved in growth was subsequently analysed by quantitative real-time PCR (qPCR). Fast- and slow-growing abalone and in vitro primary haemocyte cultures treated with different growth-stimulating factors were used. The results obtained from transcriptome analysis and qPCR revealed significant differences in gene expression between large and small abalone, and between treated and untreated haemocyte cell cultures. Throughout in vivo and in vitro qPCR experiments, the up-regulation of genes involved in the insulin signalling pathway suggests that insulin may be involved in enhanced growth rate for various H. midae tissues.
authors.affiliation: Molecular Aquatic Research Group, Department of Genetics, Stellenbosch University, Private Bag X1, Matieland 7602, South Africa.
authors.forename: Mathilde
authors.initials: M
authors.lastname: van der Merwe
authors.forename: Paolo
authors.initials: P
authors.lastname: Franchini
authors.forename: Rouvay
authors.initials: R
authors.lastname: Roodt-Wilding
mesh_terms: D000818:Animals; D017756:Aquaculture / Q000379:methods*; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D049851:Gastropoda / Q000254:growth & development* / Q000378:metabolism*; D006434:Hemocytes / Q000166:cytology / Q000378:metabolism; D007328:Insulin / Q000378:metabolism; D058977:Molecular Sequence Annotation / Q000379:methods; D008969:Molecular Sequence Data; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA / Q000662:veterinary; D013019:South Africa; D013045:Species Specificity; D059467:Transcriptome
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D007328:Insulin