title: Gene transfection and expression in the primary culture of crayfish hemocytes.
abstract: Lack of efficient gene delivery and expression methods is the major obstacle for crustacean research in the cellular level. Here, we reported the construction of an expression vector with a strong promoter from shrimp white spot syndrome virus. This vector could efficiently express foreign genes in the primary culture of crayfish hemocytes with the transfection efficiency between 5 and 10% by electroporation. Our findings provide a method for in vitro gene functional study in primary crayfish cells.
authors.affiliation: Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration (SOA), Xiamen 361005, PR China. lifang_87@163.com
authors.forename: Fang
authors.initials: F
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Ke
authors.forename: Xiumin
authors.initials: X
authors.lastname: Yan
authors.forename: Limei
authors.initials: L
authors.lastname: Xu
mesh_terms: D000818:Animals; D003400:Astacoidea / Q000235:genetics* / Q000821:virology; D018274:Electroporation; D005822:Genetic Vectors; D014162:Transfection / Q000379:methods*; D046848:White spot syndrome virus 1 / Q000235:genetics