title: Effects of licarin E on expression of matrix metalloproteinase-1 and type-1 procollagen in UVB-irradiated human skin fibroblasts.
abstract: Ultraviolet B (UVB) radiation induces photoaging by upregulating the expression of matrix metalloproteinase (MMP) and decreasing collagen synthesis in human skin cells. This study evaluated the effects of licarin E isolated from mace, the aril of Myristica fragrans Houtt., on MMP-1 and type-1 procollagen levels in UVB-irradiated human skin fibroblasts. Powdered mace extracted with 95% ethanol was used and licarin E isolated by preparative high-performance liquid chromatography. In addition, western blot analysis, reverse transcription PCR and electrophoretic mobility shift assay were used to evaluate the effects of licarin E and its molecular mechanism. It was found that licarin E attenuated UVB-induced MMP-1 expression by inactivating mitogen-activated protein kinases (MAPKs), thereby inhibiting activator protein 1. Licarin E also increased type-1 procollagen expression by stimulating transforming growth factor β (TGFβ)/Smad signaling. The findings show that licarin E positively regulates the expression of MMP-1 and type-1 procollagen in UVB-irradiated human skin fibroblasts through MAPK and TGFβ signaling, suggesting its potential as a potent antiphotoaging agent.
authors.affiliation: Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seodaemun‐gu, Seoul, 120-749, Korea.
authors.forename: Yi-Young
authors.initials: YY
authors.lastname: Kwon
authors.forename: Daeyoung
authors.initials: D
authors.lastname: Kim
authors.forename: Jaekyung
authors.initials: J
authors.lastname: Kim
authors.forename: Jae-Kwan
authors.initials: JK
authors.lastname: Hwang
mesh_terms: D002460:Cell Line; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D006801:Humans; D017705:Lignans / Q000302:isolation & purification / Q000494:pharmacology*; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism*; D026323:Myristica / Q000737:chemistry; D011347:Procollagen / Q000378:metabolism*; D012867:Skin / Q000166:cytology; D014466:Ultraviolet Rays
chemical_list: D017705:Lignans; D011347:Procollagen; C570709:licarin E; C522360:MMP1 protein, human; D020781:Matrix Metalloproteinase 1