title: Abnormal peripubertal development of the rat mammary gland following exposure in utero and during lactation to a mixture of genistein and the food contaminant vinclozolin.
abstract: The impact of early exposure to endocrine disruptor mixtures on mammary gland development is poorly known. Here, we identify the effects of a conception to weaning exposure of rats to the phytoestrogen genistein (G) and/or the antiandrogen vinclozolin (V) at 1mg/kg-d, alone or in association. Using several approaches, we found that G- and GV-exposed rats displayed significantly greater epithelial branching and proliferation, wider terminal end buds than controls at PND35, as well as ductal hyperplasia and periductal fibrosis. Focal branching defects were present in V-exposed rats. An increased ER and AR expression was observed in G- and GV- as compared to V-exposed rats at PND35. Surprisingly, a significant number of GV- and to a lesser extent, V-exposed animals displayed abnormal hyperplasic alveolar structures at PND50. Thus, gestational and lactational exposure to low doses of genistein plus vinclozolin may seriously affect peripubertal development of the rat mammary gland.
authors.affiliation: INSERM U965 UFR Médecine, Hôpital Lariboisière, Université Paris 7, F-75475 Paris, France.
authors.forename: H
authors.initials: H
authors.lastname: El Sheikh Saad
authors.forename: G
authors.initials: G
authors.lastname: Meduri
authors.forename: P
authors.initials: P
authors.lastname: Phrakonkham
authors.forename: R
authors.initials: R
authors.lastname: Bergès
authors.forename: S
authors.initials: S
authors.lastname: Vacher
authors.forename: M
authors.initials: M
authors.lastname: Djallali
authors.forename: J
authors.initials: J
authors.lastname: Auger
authors.forename: M C
authors.initials: MC
authors.lastname: Canivenc-Lavier
authors.forename: M
authors.initials: M
authors.lastname: Perrot-Applanat
mesh_terms: D000726:Androgen Antagonists / Q000633:toxicity*; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004338:Drug Combinations; D005260:Female; D005506:Food Contamination; D019833:Genistein / Q000633:toxicity*; D006965:Hyperplasia / Q000139:chemically induced / Q000473:pathology; D007774:Lactation / Q000187:drug effects*; D008321:Mammary Glands, Animal / Q000187:drug effects* / Q000254:growth & development / Q000473:pathology; D018811:Maternal Exposure / Q000009:adverse effects; D010080:Oxazoles / Q000633:toxicity*; D048789:Phytoestrogens / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D012741:Sexual Maturation / Q000187:drug effects; D014621:Vagina / Q000187:drug effects / Q000254:growth & development
chemical_list: D000726:Androgen Antagonists; D004338:Drug Combinations; D010080:Oxazoles; D048789:Phytoestrogens; D019833:Genistein; C025643:vinclozolin