title: NONOates--polyethylenimine hydrogel for controlled nitric oxide release and cell proliferation modulation.
abstract: In recent years, numerous research activities have been devoted to the controlled release of nitric oxide (NO) due to its potential as a restenosis inhibitor which inhibits the proliferation of vascular smooth muscle cells, the apoptosis of vascular endothelial cells, and aggregation of platelets. This work has demonstrated the development of a novel NO-conjugated gel system comprising of thermosensitive Pluronic F127, branched polyethylenimine (BPEI), and diazeniumdiolates (NONOates). Synthesis of conjugated Pluronic-BPEI-NONOates involved coupling of activated F127 to BPEI followed by the installation of NONOates at the secondary amine sites of branched PEI backbone under high pressure. NO-conjugated gel system, F127-BPEI-NONOates, reduced the initial burst of NO release and prolonged NO release. Furthermore, F127-BPEI-NONOates polymer coated on cell culture dish displayed much higher increase of endothelial cell proliferation and reduction of smooth muscle cell proliferation than that exhibited by non-NO releasing control. Such an NO-releasing device can operate locally and has a great potential in several biomedical applications due to high biocompatibility imparted by the conjugated F127.
authors.affiliation: Department of Chemistry, Pohang University of Science and Technology, Pohang 790-784, Korea.
authors.forename: Jihoon
authors.initials: J
authors.lastname: Kim
authors.forename: Yanggy
authors.initials: Y
authors.lastname: Lee
authors.forename: Kaushik
authors.initials: K
authors.lastname: Singha
authors.forename: Hyun Woo
authors.initials: HW
authors.lastname: Kim
authors.forename: Jae Ho
authors.initials: JH
authors.lastname: Shin
authors.forename: Seongbong
authors.initials: S
authors.lastname: Jo
authors.forename: Dong-Keun
authors.initials: DK
authors.lastname: Han
authors.forename: Won Jong
authors.initials: WJ
authors.lastname: Kim
mesh_terms: D001391:Azo Compounds / Q000737:chemistry*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D008958:Models, Molecular; D015394:Molecular Structure; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000187:drug effects*; D009569:Nitric Oxide / Q000378:metabolism* / Q000494:pharmacology*; D020442:Poloxamer / Q000737:chemistry; D011094:Polyethyleneimine / Q000737:chemistry*; D013329:Structure-Activity Relationship
chemical_list: D001391:Azo Compounds; C452539:diazeniumdiolate; D020442:Poloxamer; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D009569:Nitric Oxide; D011094:Polyethyleneimine