title: The effects of G-CSF on proliferation of mouse myocardial microvascular endothelial cells.
abstract: This paper explores the effect of granulocyte colony-stimulating factor (G-CSF) on mouse myocardial microvascular endothelial cell (CMECs) proliferation. CMECs were harvested from C57/BL6 mice. CMECs were cultured in medium containing G-CSF (0 ng/mL, 20 ng/mL, 40 ng/mL, 60 ng/mL) for five days. Proliferative activity of CMECs was examined by CCK-8 method. Hypoxia inducible factor-1 (HIF-1) and p53 expression levels was determined from the mRNA obtained by reverse transcription polymerase chain reaction (RT-PCR). Results showed that the purity quotient of the CMECs, which were cultured by the method of modified myocardial tissue explant culture, was higher than 95%. Compared with control untreated cells, the proliferative activity of CMECs and the expression level of HIF-1 mRNA in these cells were enhanced by G-CSF treatment, whereas the expression level of p53 mRNA was markedly reduced. It may be concluded that G-CSF could promote the proliferative activity of CMECs, which might be mediated by upregulation of HIF-1 and downregulation of p53.
authors.affiliation: Department of Cardiology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, 150 Jimo Road, Shanghai 200120, China; E-Mails: lijmsh912@yahoo.com.cn (J.L.); dfzhangsh912@yahoo.com.cn (D.Z.).
authors.forename: Jiming
authors.initials: J
authors.lastname: Li
authors.forename: Yunzeng
authors.initials: Y
authors.lastname: Zou
authors.forename: Junbo
authors.initials: J
authors.lastname: Ge
authors.forename: Daifu
authors.initials: D
authors.lastname: Zhang
authors.forename: Aili
authors.initials: A
authors.lastname: Guan
authors.forename: Jian
authors.initials: J
authors.lastname: Wu
authors.forename: Lei
authors.initials: L
authors.lastname: Li
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D002478:Cells, Cultured; D003331:Coronary Vessels / Q000166:cytology; D015536:Down-Regulation; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D016179:Granulocyte Colony-Stimulating Factor / Q000187:drug effects*; D051793:Hypoxia-Inducible Factor 1 / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D055806:Microvessels / Q000166:cytology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism
chemical_list: D051793:Hypoxia-Inducible Factor 1; D012333:RNA, Messenger; D016159:Tumor Suppressor Protein p53; D016179:Granulocyte Colony-Stimulating Factor