title: Activated platelet-derived growth factor β receptor and Ras-mitogen-activated protein kinase pathway in natural bovine urinary bladder carcinomas.
abstract: Bovine papillomavirus types 1 or 2 (BPV-1/2) are involved in the aetiopathogenesis of bovine urinary bladder cancer. BPV-1/2 E5 activates the platelet-derived growth factor β receptor (PDGFβR). The aim of this study was to analyse the Ras/mitogen-activated protein kinase (MAPK) pathway in relation to activation of PDGFβR in natural bovine urinary bladder carcinomas. Co-immunoprecipitation and Western blot analysis demonstrated that recruitment of growth factor receptor bound protein 2 (GRB-2) and Sos-1 to the activated PDGFβR was increased in carcinomas compared to normal tissues. Higher grade bovine urinary bladder carcinomas were associated with activation of Ras, but not with activation of downstream mitogen-activated protein kinase/extracellular signal-regulated kinase (Mek 1/2) or extracellular signal-regulated kinase (Erk 1/2).
authors.affiliation: Department of Pathology and Animal Health, Faculty of Veterinary Medicine, University of Naples Federico II, Via F. Delpino, 1, 80137 Napoli, Italy.
authors.forename: Annunziata
authors.initials: A
authors.lastname: Corteggio
authors.forename: Ornella
authors.initials: O
authors.lastname: Di Geronimo
authors.forename: Sante
authors.initials: S
authors.lastname: Roperto
authors.forename: Franco
authors.initials: F
authors.lastname: Roperto
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Borzacchiello
mesh_terms: D000818:Animals; D002277:Carcinoma / Q000378:metabolism / Q000662:veterinary*; D002417:Cattle; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology; D020935:MAP Kinase Signaling System / Q000502:physiology*; D020929:Mitogen-Activated Protein Kinase Kinases / Q000378:metabolism*; D020797:Receptor, Platelet-Derived Growth Factor beta / Q000235:genetics / Q000378:metabolism*; D001749:Urinary Bladder Neoplasms / Q000378:metabolism / Q000662:veterinary*; D018631:ras Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D020797:Receptor, Platelet-Derived Growth Factor beta; D020929:Mitogen-Activated Protein Kinase Kinases; D018631:ras Proteins