title: Effect of endodontic irrigating solutions on the microhardness and roughness of root canal dentin: an in vitro study.
abstract: BACKGROUND
  To evaluate the effect of widely used endodontic irrigating solutions on root dentin microhardness and surface roughness.
  
  
  METHODS
  One hundred twenty, non-carious extracted human permanent incisor teeth were selected. The crowns of the teeth were sectioned and the roots were separated longitudinally to get 240 specimens. These specimens were then divided into six groups according to the irrigating solutions used. The solutions used were 5% and 2.5% NaOCl solutions, 3% H2 O2 , 17% EDTA solution, 0.2% chlorhexidine gluconate, and distilled water. Then, the specimens were subjected to microhardness and roughness testing. The data were analyzed using ANOVA and Tukey's multiple comparison tests.
  
  
  RESULTS
  The results of this study indicated that all irrigation solutions, except 0.2% chlorhexidine gluconate, decreased the microhardness of root dentin, and 3% H2 O2 and 0.2% chlorhexidine gluconate had no effect on surface roughness.
  
  
  CONCLUSIONS
  Within the limitation of this study, it is concluded that 0.2% chlorhexidine gluconate seems to be an appropriate irrigation solution, because of its harmless effect on the microhardness and surface roughness of root canal dentin.
authors.affiliation: Department of Conservative & Endodontics, KLE's V K Institute of Dental Sciences, Belgaum, Karnataka, India. chetsrpats@yahoo.com
authors.forename: Chetan R
authors.initials: CR
authors.lastname: Patil
authors.forename: Veerendra
authors.initials: V
authors.lastname: Uppin
mesh_terms: D000704:Analysis of Variance; D002710:Chlorhexidine / Q000031:analogs & derivatives / Q000494:pharmacology; D003646:Debridement / Q000379:methods*; D003786:Dental Pulp Cavity / Q000187:drug effects* / Q000648:ultrastructure; D003804:Dentin / Q000187:drug effects* / Q000648:ultrastructure; D004492:Edetic Acid / Q000494:pharmacology; D006244:Hardness; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D012388:Root Canal Irrigants / Q000494:pharmacology*; D012973:Sodium Hypochlorite / Q000494:pharmacology; D018709:Statistics, Nonparametric; D013499:Surface Properties; D007507:Therapeutic Irrigation / Q000379:methods*; D014092:Tooth Root
chemical_list: D012388:Root Canal Irrigants; D004492:Edetic Acid; D006861:Hydrogen Peroxide; D012973:Sodium Hypochlorite; C010882:chlorhexidine gluconate; D002710:Chlorhexidine