title: Characterization of Haemophilus parasuis isolated from Brazilian swine through serotyping, AFLP and PFGE.
abstract: Haemophilus parasuis infection in pigs is characterized by fibrinous polyserositis, arthritis and meningitis. Despite the fact that traditional diagnosis is based on herd history, clinical signs, bacterial isolation and serotyping, molecular-based methods are alternatives for species-specific tests and epidemiological studies. The aim of this study was to characterize H. parasuis field strains from different states of Brazil, employing serotyping and genotyping methods. Serotyping revealed that serovar 4 was the most prevalent (26.1%), followed by serovars 5 (17.4%), 14 (8.7%), 13 (4.4%) and 2 (4.4%), whereas 39% of the strains were considered as untypeable. AFLP with a single enzyme and PFGE were able to type all isolates tested, generating 34 and 20 different profiles, respectively, including untypeable strains. Besides the slightly higher discrimination index presented by AFLP, PFGE with Not I restriction enzyme showed a better correlation with epidemiological data, grouping strains of the same serovar, animal or farm origin. The results indicated AFLP and PFGE as valuable tools for typing H. parasuis isolates collected in Brazil.
authors.affiliation: Laboratório de Sanidade Suína e Virologia, Faculdade de Medicina Veterinária e Zootecnia (FMVZ), Universidade de São Paulo (USP), São Paulo, Brazil.
authors.forename: Karina Salvagni
authors.initials: KS
authors.lastname: Castilla
authors.forename: Débora Dirani Sena
authors.initials: DD
authors.lastname: de Gobbi
authors.forename: Luisa Zanolli
authors.initials: LZ
authors.lastname: Moreno
authors.forename: Renata
authors.initials: R
authors.lastname: Paixão
authors.forename: Tania Alen
authors.initials: TA
authors.lastname: Coutinho
authors.forename: José Lúcio
authors.initials: JL
authors.lastname: dos Santos
authors.forename: Andrea Micke
authors.initials: AM
authors.lastname: Moreno
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis / Q000662:veterinary*; D000818:Animals; D001938:Brazil / Q000453:epidemiology; D016521:Electrophoresis, Gel, Pulsed-Field / Q000662:veterinary*; D015964:Gene Expression Regulation, Bacterial; D006192:Haemophilus Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D044137:Haemophilus parasuis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000382:microbiology*