title: Suppression of a NAC-like transcription factor gene improves boron-toxicity tolerance in rice.
abstract: We identified a gene responsible for tolerance to boron (B) toxicity in rice (Oryza sativa), named BORON EXCESS TOLERANT1. Using recombinant inbred lines derived from the B-toxicity-sensitive indica-ecotype cultivar IR36 and the tolerant japonica-ecotype cultivar Nekken 1, the region responsible for tolerance to B toxicity was narrowed to 49 kb on chromosome 4. Eight genes are annotated in this region. The DNA sequence in this region was compared between the B-toxicity-sensitive japonica cultivar Wataribune and the B-toxicity-tolerant japonica cultivar Nipponbare by eco-TILLING analysis and revealed a one-base insertion mutation in the open reading frame sequence of the gene Os04g0477300. The gene encodes a NAC (NAM, ATAF, and CUC)-like transcription factor and the function of the transcript is abolished in B-toxicity-tolerant cultivars. Transgenic plants in which the expression of Os04g0477300 is abolished by RNA interference gain tolerance to B toxicity.
authors.affiliation: Laboratory of Plant Nutrition, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.
authors.forename: Kumiko
authors.initials: K
authors.lastname: Ochiai
authors.forename: Akifumi
authors.initials: A
authors.lastname: Shimizu
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Okumoto
authors.forename: Toru
authors.initials: T
authors.lastname: Fujiwara
authors.forename: Toru
authors.initials: T
authors.lastname: Matoh
mesh_terms: D000222:Adaptation, Physiological / Q000187:drug effects / Q000235:genetics*; D001895:Boron / Q000633:toxicity*; D018744:DNA, Plant / Q000235:genetics; D017343:Genes, Plant / Q000235:genetics; D008969:Molecular Sequence Data; D012275:Oryza / Q000187:drug effects* / Q000235:genetics*; D020161:Physical Chromosome Mapping; D010940:Plant Proteins / Q000235:genetics*; D011110:Polymorphism, Genetic; D034622:RNA Interference / Q000187:drug effects; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013489:Suppression, Genetic / Q000187:drug effects*; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D015533:Transcriptional Activation / Q000187:drug effects / Q000235:genetics
chemical_list: D018744:DNA, Plant; D010940:Plant Proteins; D014157:Transcription Factors; D001895:Boron