title: Gold nanoparticle-based colorimetric detection of kanamycin using a DNA aptamer.
abstract: A selective kanamycin-binding single-strand DNA (ssDNA) aptamer (TGGGGGTTGAGGCTAAGCCGA) was discovered through in vitro selection using affinity chromatography with kanamycin-immobilized sepharose beads. The selected aptamer has a high affinity for kanamycin and also for kanamycin derivatives such as kanamycin B and tobramycin. The dissociation constants (K(d) [kanamycin]=78.8 nM, K(d) [kanamycin B]=84.5 nM, and K(d) [tobramycin]=103 nM) of the new aptamer were determined by fluorescence intensity analysis using 5'-fluorescein amidite (FAM) modification. Using this aptamer, kanamycin was detected down to 25 nM by the gold nanoparticle-based colorimetric method. Because the designed colorimetric method is simple, easy, and visible to the naked eye, it has advantages that make it useful for the detection of kanamycin. Furthermore, the selected new aptamer has many potential applications as a bioprobe for the detection of kanamycin, kanamycin B, and tobramycin in pharmaceutical preparations and food products.
authors.affiliation: Department of Chemistry, Pohang University of Science and Technology, Pohang, Gyungbuk 790-784, South Korea.
authors.forename: Kyung-Mi
authors.initials: KM
authors.lastname: Song
authors.forename: Minseon
authors.initials: M
authors.lastname: Cho
authors.forename: Hunho
authors.initials: H
authors.lastname: Jo
authors.forename: Kyoungin
authors.initials: K
authors.lastname: Min
authors.forename: Sung Ho
authors.initials: SH
authors.lastname: Jeon
authors.forename: Taisun
authors.initials: T
authors.lastname: Kim
authors.forename: Min Su
authors.initials: MS
authors.lastname: Han
authors.forename: Ja Kang
authors.initials: JK
authors.lastname: Ku
authors.forename: Changill
authors.initials: C
authors.lastname: Ban
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis*; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D003124:Colorimetry / Q000379:methods*; D004277:DNA, Single-Stranded / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D007612:Kanamycin / Q000032:analysis*; D007700:Kinetics; D053768:Metal Nanoparticles / Q000737:chemistry*; D004364:Pharmaceutical Preparations / Q000737:chemistry; D014031:Tobramycin / Q000032:analysis
chemical_list: D000900:Anti-Bacterial Agents; D052157:Aptamers, Nucleotide; D004277:DNA, Single-Stranded; D004364:Pharmaceutical Preparations; D007612:Kanamycin; D006046:Gold; D014031:Tobramycin