title: Apoptotic bone marrow CD34+ cells in cirrhotic patients.
abstract: OBJECTIVE
  To access the frequency and level of apoptotic CD34+ cells isolated from the marrow fluid of patients with post-hepatitis cirrhosis.
  
  
  METHODS
  The frequency of bone marrow CD34+ cells and apoptotic bone marrow CD34+ cells in 31 in-patients with post-hepatitis cirrhosis (cirrhosis group), and 15 out-patients without liver or blood disorders (control group) was calculated by flow cytometry. Parameters were collected to evaluate liver functions of patients in cirrhosis group.
  
  
  RESULTS
  The percentage of normal bone marrow CD34+ cells was 6.30% ± 2.48% and 1.87% ± 0.53% (t = 3.906, P < 0.01) while that of apoptotic marrow CD34+ cells was 15.00% ± 15.81% and 5.73% ± 1.57% (t = 2.367, P < 0.05) in cirrhosis and control groups, respectively. The percentage of apoptotic marrow CD34+ cells was 6.25% ± 3.30% and 20.92 ± 18.5% (t = 2.409, P < 0.05) in Child-Pugh A and Child-Pugh B + C cirrhotic patients, respectively. The percentage of late apoptotic marrow CD34+ cells was positively correlated with the total bilirubin and aspartate aminotransferase serum levels in patients with cirrhosis.
  
  
  CONCLUSIONS
  The status of CD34+ marrow cells in cirrhotic patients may suggest that the ability of hematopoietic progenitor cells to transform into mature blood cells is impaired.
authors.affiliation: Department of Infectious Diseases, Second Affiliated Hospital of Medical School of Xi'an Jiaotong University, 157 Xiwu Road, Xi'an 710004, Shaanxi Province, China. dang212@126.com
authors.forename: Shuang-Suo
authors.initials: SS
authors.lastname: Dang
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Wang
authors.forename: Ning
authors.initials: N
authors.lastname: Gao
authors.forename: Shun-Da
authors.initials: SD
authors.lastname: Wang
authors.forename: Mei
authors.initials: M
authors.lastname: Li
authors.forename: La-Yang
authors.initials: LY
authors.lastname: Liu
authors.forename: Ming-Zhun
authors.initials: MZ
authors.lastname: Sun
authors.forename: Tao
authors.initials: T
authors.lastname: Dong
mesh_terms: D000328:Adult; D018952:Antigens, CD34 / Q000276:immunology*; D017209:Apoptosis / Q000276:immunology*; D001663:Bilirubin / Q000097:blood; D001854:Bone Marrow Cells / Q000276:immunology* / Q000502:physiology*; D005260:Female; D006521:Hepatitis, Chronic / Q000150:complications / Q000276:immunology; D006801:Humans; D008103:Liver Cirrhosis / Q000097:blood / Q000209:etiology / Q000276:immunology* / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D000637:Transaminases / Q000097:blood
chemical_list: D018952:Antigens, CD34; D000637:Transaminases; D001663:Bilirubin