title: Biomarker responsiveness in different tissues of caged Ruditapes philippinarum and its use within an integrated sediment quality assessment.
abstract: Biomarkers comprising activities of biotransformation enzymes (ethoxyresorufin-O-deethylase -EROD-, dibenzylfluorescein dealkylase -DBF-, glutathione S-transferase -GST), antioxidant enzymes (glutathione reductase -GR- and glutathione peroxidase -GPX), lipid peroxidation -LPO- and DNA strand breaks were analyzed in the clam Ruditapes philippinarum caged at Cádiz Bay, Santander Bay and Las Palmas de Gran Canaria (LPGC) Port (Spain). Sediments were characterized. Digestive gland was the most sensitive tissue to sediment contamination. In Cádiz Bay, changes in LPO regarding day 0 were related with metals. In LPGC Port, DBF, EROD, and GST activity responses suggested the presence of undetermined contaminants which might have led to DNA damage. In Santander Bay, PAHs were related with EROD activity, organic and metal contamination was found to be associated with GR and GST activities and DNA damage presented significant (p < 0.05) induction. R. philippinarum was sensitive to sediment contamination at biochemical level. Biomarkers allowed chemical exposure and sediment quality assessment.
authors.affiliation: UNITWIN/UNESCO/WiCoP, Departamento de Química Física, Universidad de Cádiz, Facultad de Ciencias del Mar y Ambientales, Campus Universitario de Puerto Real, 11510 Puerto Real, Cádiz, Spain. julia.ramosgomez@uca.es
authors.forename: Julia
authors.initials: J
authors.lastname: Ramos-Gómez
authors.forename: Alberto
authors.initials: A
authors.lastname: Coz
authors.forename: Javier R
authors.initials: JR
authors.lastname: Viguri
authors.forename: Angel
authors.initials: A
authors.lastname: Luque
authors.forename: M Laura
authors.initials: ML
authors.lastname: Martín-Díaz
authors.forename: T Ángel
authors.initials: TÁ
authors.lastname: DelValls
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis* / Q000378:metabolism; D049872:Bivalvia / Q000737:chemistry* / Q000201:enzymology / Q000235:genetics; D019363:Cytochrome P-450 CYP1A1 / Q000032:analysis / Q000378:metabolism; D004784:Environmental Monitoring / Q000379:methods*; D019015:Geologic Sediments / Q000032:analysis*; D005979:Glutathione Peroxidase / Q000032:analysis / Q000378:metabolism; D005980:Glutathione Reductase / Q000032:analysis / Q000378:metabolism; D005982:Glutathione Transferase / Q000032:analysis / Q000378:metabolism; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000378:metabolism
chemical_list: D015415:Biomarkers; D011084:Polycyclic Aromatic Hydrocarbons; D014874:Water Pollutants, Chemical; D005979:Glutathione Peroxidase; D019363:Cytochrome P-450 CYP1A1; D005980:Glutathione Reductase; D005982:Glutathione Transferase