title: Development of Porphyromonas gingivalis-specific quantitative real-time PCR primers based on the nucleotide sequence of rpoB.
abstract: Species-specific quantitative real-time PCR (qPCR) primers were developed for the detection of Porphyromonas gingivalis. These primers, Pg-F/Pg-R, were designed based on the nucleotide sequences of RNA polymerase β-subunit gene (rpoB). Species-specific amplicons were obtained from the tested P. gingivalis strains but not in any of the other strains (46 strains of 46 species). The qPCR primers could detect as little as 4 fg of P. gingivalis chromosomal DNA. These findings suggest that these qPCR primers are suitable for applications in epidemiological studies.
authors.affiliation: Department of Oral Biochemistry, Chosun University, Gwangju 501-759, Republic of Korea.
authors.forename: Soon-Nang
authors.initials: SN
authors.lastname: Park
authors.forename: Jae-Yoon
authors.initials: JY
authors.lastname: Park
authors.forename: Joong-Ki
authors.initials: JK
authors.lastname: Kook
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D016866:Bacteroidaceae Infections / Q000175:diagnosis / Q000382:microbiology; D017931:DNA Primers / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D016966:Porphyromonas gingivalis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit