title: Chronic cardiac pressure overload induces adrenal medulla hypertrophy and increased catecholamine synthesis.
abstract: Increased activity of the sympathetic system is an important feature contributing to the pathogenesis and progression of chronic heart failure. While the mechanisms and consequences of enhanced norepinephrine release from sympathetic nerves have been intensely studied, the role of the adrenal gland in the development of cardiac hypertrophy and progression of heart failure is less well known. Thus, the aim of the present study was to determine the effect of chronic cardiac pressure overload in mice on adrenal medulla structure and function. Cardiac hypertrophy was induced in wild-type mice by transverse aortic constriction (TAC) for 8 weeks. After TAC, the degree of cardiac hypertrophy correlated significantly with adrenal weight and adrenal catecholamine storage. In the medulla, TAC caused an increase in chromaffin cell size but did not result in chromaffin cell proliferation. Ablation of chromaffin α(2C)-adrenoceptors did not affect adrenal weight or epinephrine synthesis. However, unilateral denervation of the adrenal gland completely prevented adrenal hypertrophy and increased catecholamine synthesis. Transcriptome analysis of microdissected adrenal medulla identified 483 up- and 231 downregulated, well-annotated genes after TAC. Among these genes, G protein-coupled receptor kinases 2 (Grk2) and 6 and phenylethanolamine N-methyltransferase (Pnmt) were significantly upregulated by TAC. In vitro, acetylcholine-induced Pnmt and Grk2 expression as well as enhanced epinephrine content was prevented by inhibition of nicotinic acetylcholine receptors and Ca(2+)/calmodulin-dependent signaling. Thus, activation of preganglionic sympathetic nerves innervating the adrenal medulla plays an essential role in inducing adrenal hypertrophy, enhanced catecholamine synthesis and induction of Grk2 expression after cardiac pressure overload.
authors.affiliation: Institute of Experimental and Clinical Pharmacology and Toxicology, University of Freiburg, Albertstrasse 25, Freiburg, Germany.
authors.forename: Johanna
authors.initials: J
authors.lastname: Schneider
authors.forename: Achim
authors.initials: A
authors.lastname: Lother
authors.forename: Lutz
authors.initials: L
authors.lastname: Hein
authors.forename: Ralf
authors.initials: R
authors.lastname: Gilsbach
mesh_terms: D000311:Adrenal Glands / Q000294:innervation; D000313:Adrenal Medulla / Q000473:pathology*; D000818:Animals; D001794:Blood Pressure; D003714:Denervation; D004837:Epinephrine / Q000096:biosynthesis*; D054769:G-Protein-Coupled Receptor Kinase 2 / Q000235:genetics; D020869:Gene Expression Profiling; D006984:Hypertrophy; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000032:analysis; D018341:Receptors, Adrenergic, alpha-2 / Q000502:physiology
chemical_list: D012333:RNA, Messenger; D018341:Receptors, Adrenergic, alpha-2; C496568:GRK2 protein, mouse; D054769:G-Protein-Coupled Receptor Kinase 2; D004837:Epinephrine