title: Mutagenic adaptive response to high-LET radiation in human lymphoblastoid cells exposed to low doses of heavy-ion radiation.
abstract: Adaptive response (AR) and bystander effect are two important phenomena involved in biological responses to low doses of ionizing radiation (IR). Furthermore, there is a strong interest in better understanding the biological effects of high-LET radiation. We previously demonstrated the ability of low doses of X-rays to induce an AR to challenging heavy-ion radiation [8]. In this study, we assessed in vitro the ability of priming low doses (0.01Gy) of heavy-ion radiation to induce a similar AR to a subsequent challenging dose (1-4Gy) of high-LET IR (carbon-ion: 20 and 40keV/μm, neon-ion: 150keV/μm) in TK6, AHH-1 and NH32 cells. Our results showed that low doses of high-LET radiation can induce an AR characterized by lower mutation frequencies at hypoxanthine-guanine phosphoribosyl transferase locus and faster DNA repair kinetics, in cells expressing p53.
authors.affiliation: Radiation Risk Reduction Research Program, National Institute of Radiological Sciences, Anagawa 4-9-1, Inage-ku, Chiba 263-8555, Japan. vares@nirs.go.jp
authors.forename: Guillaume
authors.initials: G
authors.lastname: Varès
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
authors.forename: Kaoru
authors.initials: K
authors.lastname: Tanaka
authors.forename: Ayana
authors.initials: A
authors.lastname: Kakimoto
authors.forename: Kyomi
authors.initials: K
authors.lastname: Eguchi-Kasai
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Nenoi
mesh_terms: D000220:Adaptation, Biological; D002460:Cell Line; D004260:DNA Repair; D020450:Heavy Ions; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics; D018499:Linear Energy Transfer; D008214:Lymphocytes / Q000528:radiation effects*; D009154:Mutation; D009356:Neon; D011829:Radiation Dosage; D011839:Radiation, Ionizing
chemical_list: D009356:Neon; D007041:Hypoxanthine Phosphoribosyltransferase