title: Oral immunization with live Lactococcus lactis expressing rotavirus VP8 subunit induces specific immune response in mice.
abstract: Rotaviruses are the major cause of worldwide infectious diarrhea in children and vaccination is considered to be the most effective way to control these infections. The development of a mucosal live vaccine using the food-grade lactic acid bacteria Lactococcus lactis as antigen vehicle is an attractive and safe vaccination strategy against rotavirus. In this study, the construction of recombinant L. lactis strains able to produce the rotavirus spike-protein subunit VP8 in cytoplasmic, secreted and cell wall-anchored forms is reported. Evaluation of the immune response generated after immunization was conducted in a mouse model. The present study shows that animals inoculated orally with the L. lactis strain producing the cytoplasmic form of VP8 (LL1) developed significant levels of intestinal IgA antibodies while animals receiving L. lactis producing the cell wall-anchored VP8 form (LL3) exhibited anti-VP8 antibodies at both intestinal and systemic levels. Furthermore, it was observed that intestinal antibodies of the LL1-treated group and serum antibodies of the LL3-treated group were able to block rotavirus infection by 50% and 100%, respectively. These encouraging results represent a step towards the development of a new and safe mucosal vaccine against rotavirus.
authors.affiliation: Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET) and Departamento de Microbiología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, (S2002LRK) Rosario, Argentina.
authors.forename: Belkis
authors.initials: B
authors.lastname: Marelli
authors.forename: Ana Rosa
authors.initials: AR
authors.lastname: Perez
authors.forename: Claudia
authors.initials: C
authors.lastname: Banchio
authors.forename: Diego
authors.initials: D
authors.lastname: de Mendoza
authors.forename: Christian
authors.initials: C
authors.lastname: Magni
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D018928:Immunity, Mucosal; D013294:Lactococcus lactis / Q000235:genetics / Q000276:immunology*; D008253:Macaca mulatta; D051379:Mice; D008807:Mice, Inbred BALB C; D014677:Pharmaceutical Vehicles / Q000494:pharmacology; D016601:RNA-Binding Proteins / Q000235:genetics / Q000276:immunology*; D012401:Rotavirus / Q000276:immunology*; D012400:Rotavirus Infections / Q000276:immunology / Q000517:prevention & control*; D022243:Rotavirus Vaccines / Q000008:administration & dosage / Q000276:immunology*; D014611:Vaccination; D014613:Vaccines, Attenuated / Q000276:immunology; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D014677:Pharmaceutical Vehicles; D016601:RNA-Binding Proteins; D022243:Rotavirus Vaccines; D014613:Vaccines, Attenuated; D017361:Viral Nonstructural Proteins; C079234:NS35 protein, rotavirus