title: Characterization of and host response to tyramine substituted-hyaluronan enriched fascia extracellular matrix.
abstract: Naturally-occurring biomaterial scaffolds derived from extracellular matrix (ECM) have been previously investigated for soft tissue repair. We propose to enrich fascia ECM with high molecular weight tyramine substituted-hyaluronan (TS-HA) to modulate inflammation associated with implantation and enhance fibroblast infiltration. As critical determinants of constructive remodeling, the host inflammatory response and macrophage polarization to TS-HA enriched fascia were characterized in a rat abdominal wall model. TS-HA treated fascia with cross-linking had a similar lymphocyte (P = 0.11) and plasma cell (P = 0.13) densities, greater macrophage (P = 0.001) and giant cell (P < 0.0001) densities, and a lower density of fibroblast-like cells (P < 0.0001) than water treated controls. Treated fascia, with or without cross-linking, exhibited a predominantly M2 pro-remodeling macrophage profile similar to water controls (P = 0.82), which is suggestive of constructive tissue remodeling. Our findings demonstrated that HA augmentation can alter the host response to an ECM, but the appropriate concentration and molecular weight needed to minimize chronic inflammation within the scaffold remains to be determined.
authors.affiliation: Department of Biomedical Engineering, Lerner Research Institute, Cleveland Clinic, 9500 Euclid Avenue, ND20, Cleveland, OH 44195, USA.
authors.forename: Likang
authors.initials: L
authors.lastname: Chin
authors.forename: Anthony
authors.initials: A
authors.lastname: Calabro
authors.forename: E Rene
authors.initials: ER
authors.lastname: Rodriguez
authors.forename: Carmela D
authors.initials: CD
authors.lastname: Tan
authors.forename: Esteban
authors.initials: E
authors.lastname: Walker
authors.forename: Kathleen A
authors.initials: KA
authors.lastname: Derwin
mesh_terms: D034861:Abdominal Wall / Q000502:physiology; D000293:Adolescent; D000328:Adult; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D005109:Extracellular Matrix / Q000737:chemistry / Q000187:drug effects; D005205:Fascia / Q000737:chemistry / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry* / Q000494:pharmacology; D008297:Male; D008875:Middle Aged; D051381:Rats; D011917:Rats, Inbred Lew; D013566:Sympathomimetics / Q000737:chemistry / Q000494:pharmacology; D054457:Tissue Scaffolds / Q000737:chemistry*; D014439:Tyramine / Q000737:chemistry / Q000494:pharmacology*; D055815:Young Adult
chemical_list: D001672:Biocompatible Materials; D013566:Sympathomimetics; D006820:Hyaluronic Acid; D014439:Tyramine