title: Regulation of PBX3 expression by androgen and Let-7d in prostate cancer.
abstract: BACKGROUND
  The pre-leukemia transcription factor 3 (PBX) is part of the PBX family of transcription factors, which is known to regulate genes involved in differentiation of urogenital organs and steroidogenesis. This is of interest with regard to prostate cancer progression as regulation of steroidogenesis is one of the mechanisms involved in the development of castration-resistant prostate cancer. In light of this we wanted to investigate the possible involvement of androgen regulation of PBX3 expression in prostate cancer.
  
  
  RESULTS
  In this study, we show that PBX3 is post-transcriptionally regulated by androgen in prostate cancer cells and that the effect might be independent of the androgen receptor. Furthermore, PBX3 was identified as a target of Let-7d, an androgen regulated microRNA. Let-7d was down-regulated in malignant compared to benign prostate tissue, whereas up-regulation of PBX3 expression was observed.
  
  
  CONCLUSIONS
  We demonstrate that PBX3 is up-regulated in prostate cancer and post- transcriptionally regulated by androgen through Let-7d.
authors.affiliation: Faculty Division Aker University Hospital, University of Oslo, Aker, N-0514 Oslo, Norway.
authors.forename: Håkon
authors.initials: H
authors.lastname: Ramberg
authors.forename: Ayham
authors.initials: A
authors.lastname: Alshbib
authors.forename: Viktor
authors.initials: V
authors.lastname: Berge
authors.forename: Aud
authors.initials: A
authors.lastname: Svindland
authors.forename: Kristin Austlid
authors.initials: KA
authors.lastname: Taskén
mesh_terms: D000728:Androgens / Q000494:pharmacology*; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000473:pathology / Q000503:physiopathology*; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D011944:Receptors, Androgen / Q000378:metabolism
chemical_list: D000728:Androgens; D018398:Homeodomain Proteins; D035683:MicroRNAs; D011518:Proto-Oncogene Proteins; D011944:Receptors, Androgen; C487931:mirnlet7 microRNA, human; C090466:proto-oncogene protein Pbx3