title: A glycosidic spinasterol from Koreana stewartia promotes procollagen production and inhibits matrix metalloproteinase-1 expression in UVB-irradiated human dermal fibroblasts.
abstract: Methanol extract of Koreana stewartia leaves (SKE) stimulated collagen production in ultraviolet-B (UVB)-irradiated human fibroblast cells. An active compound was isolated from SKE by successive partitioning and chromatography, and the chemical structure was determined to be 3-O-β-D-glucopyranosylspinasterol (spinasterol-Glc) by spectroscopic characterization. Spinasterol-Glc increased collagen production in the supernatant of UVB-irradiated dermal fibroblast cell cultures in a dose-dependent manner. The effects of spinasteol-Glc on expression of procollagen and matrix metalloproteinase-1 (MMP-1) were further evaluated. We found that the compound stimulated collagen production in UVB-treated fibroblasts than in vehicle-treated control cells by about 3-fold. In addition, we also demonstrate that the compound increased the mRNA and protein levels of procollagen in UVB-treated fibroblast cells, while it inhibited expression of MMP-1. These results indicate that spinasterol-Glc protects fibroblast cells from the adverse effects of UV radiation via stimulation of procollagen synthesis as well as inhibition of MMP-1 expression. Spinasterol-Glc may be useful in the future development of therapeutic and cosmetic applications.
authors.affiliation: Graduate School of Biotechnology and College of Life Science, Kyung Hee University, Korea.
authors.forename: Tae Hoon
authors.initials: TH
authors.lastname: Lee
authors.forename: Sang Min
authors.initials: SM
authors.lastname: Lee
authors.forename: Dae-Young
authors.initials: DY
authors.lastname: Lee
authors.forename: Youngsook
authors.initials: Y
authors.lastname: Son
authors.forename: Dae Kyun
authors.initials: DK
authors.lastname: Chung
authors.forename: Nam-In
authors.initials: NI
authors.lastname: Baek
authors.forename: Jiyoung
authors.initials: J
authors.lastname: Kim
mesh_terms: D001483:Base Sequence; D015153:Blotting, Western; D002478:Cells, Cultured; D017931:DNA Primers; D005347:Fibroblasts / Q000201:enzymology / Q000378:metabolism / Q000528:radiation effects; D006801:Humans; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism*; D011347:Procollagen / Q000096:biosynthesis*; D012867:Skin / Q000201:enzymology / Q000378:metabolism / Q000528:radiation effects*; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013265:Stigmasterol / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology; D028242:Theaceae / Q000737:chemistry*
chemical_list: D017931:DNA Primers; D011347:Procollagen; C031312:spinasterol; D013265:Stigmasterol; D020781:Matrix Metalloproteinase 1