title: Aromatic aldehyde and hydrazine activated peptide coated quantum dots for easy bioconjugation and live cell imaging.
abstract: We present a robust scheme for preparation of semiconductor quantum dots (QDs) and cognate partners in a conjugation ready format. Our approach is based on bis-aryl hydrazone bond formation mediated by aromatic aldehyde and hydrazinonicotinate acetone hydrazone (HyNic) activated peptide coated quantum dots. We demonstrate controlled preparation of antibody--QD bioconjugates for specific targeting of endogenous epidermal growth factor receptors in breast cancer cells and for single QD tracking of transmembrane proteins via an extracellular epitope. The same approach was also used for optical mapping of RNA polymerases bound to combed genomic DNA in vitro.
authors.affiliation: Department of Chemistry and Biochemistry, California NanoSystems Institute, University of California at Los Angeles, Los Angeles, California 90095, United States. gopal@chem.ucla.edu
authors.forename: Gopal
authors.initials: G
authors.lastname: Iyer
authors.forename: Fabien
authors.initials: F
authors.lastname: Pinaud
authors.forename: Jianmin
authors.initials: J
authors.lastname: Xu
authors.forename: Yuval
authors.initials: Y
authors.lastname: Ebenstein
authors.forename: Jack
authors.initials: J
authors.lastname: Li
authors.forename: Jessica
authors.initials: J
authors.lastname: Chang
authors.forename: Maxime
authors.initials: M
authors.lastname: Dahan
authors.forename: Shimon
authors.initials: S
authors.lastname: Weiss
mesh_terms: D000447:Aldehydes / Q000737:chemistry*; D000818:Animals; D000906:Antibodies / Q000276:immunology; D016466:CHO Cells; D045744:Cell Line, Tumor; D002470:Cell Survival; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000737:chemistry*; D006224:Cricetinae; D003412:Cricetulus; D066246:ErbB Receptors / Q000276:immunology; D005260:Female; D006367:HeLa Cells; D006801:Humans; D006834:Hydrazines / Q000737:chemistry*; D008422:Materials Testing; D057054:Molecular Imaging / Q000379:methods*; D015394:Molecular Structure; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry*; D045663:Quantum Dots; D012666:Semiconductors
chemical_list: D000447:Aldehydes; D000906:Antibodies; D020099:Coated Materials, Biocompatible; D006834:Hydrazines; D010455:Peptides; D066246:ErbB Receptors