title: Clinical implementation of whole-genome array CGH as a first-tier test in 5080 pre and postnatal cases.
abstract: BACKGROUND
  Array comparative genomic hybridization (CGH) is currently the most powerful method for detecting chromosomal alterations in pre and postnatal clinical cases. In this study, we developed a BAC based array CGH analysis platform for detecting whole genome DNA copy number changes including specific micro deletion and duplication chromosomal disorders. Additionally, we report our experience with the clinical implementation of our array CGH analysis platform. Array CGH was performed on 5080 pre and postnatal clinical samples from patients referred with a variety of clinical phenotypes.
  
  
  RESULTS
  A total of 4073 prenatal cases (4033 amniotic fluid and 40 chorionic villi specimens) and 1007 postnatal cases (407 peripheral blood and 600 cord blood) were studied with complete concordance between array CGH, karyotype and fluorescence in situ hybridization results. Among 75 positive prenatal cases with DNA copy number variations, 60 had an aneuploidy, seven had a deletion, and eight had a duplication. Among 39 positive postnatal cases samples, five had an aneuploidy, 23 had a deletion, and 11 had a duplication.
  
  
  CONCLUSIONS
  This study demonstrates the utility of using our newly developed whole-genome array CGH as first-tier test in 5080 pre and postnatal cases. Array CGH has increased the ability to detect segmental deletion and duplication in patients with variable clinical features and is becoming a more powerful tool in pre and postnatal diagnostics.
authors.affiliation: MG MED, Inc., Seoul, Korea.
authors.forename: Sang-Jin
authors.initials: SJ
authors.lastname: Park
authors.affiliation: MG MED, Inc., Seoul, Korea.
authors.forename: Eun Hye
authors.initials: EH
authors.lastname: Jung
authors.affiliation: MG MED, Inc., Seoul, Korea.
authors.forename: Ran-Suk
authors.initials: RS
authors.lastname: Ryu
authors.affiliation: MACROGEN, Inc, Seoul, Korea.
authors.forename: Hyun Woong
authors.initials: HW
authors.lastname: Kang
authors.affiliation: Department of Medical Genetics, Ajou University School of Medicine, Suwon, Korea.
authors.forename: Jung-Min
authors.initials: JM
authors.lastname: Ko
authors.affiliation: Department of Medical Genetics, Ajou University School of Medicine, Suwon, Korea.
authors.forename: Hyon J
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: Department of Pediatrics, School of Medicine, Pusan National University Children's Hospital, Yangsan, Korea.
authors.forename: Chong Kun
authors.initials: CK
authors.lastname: Cheon
authors.affiliation: Department of Laboratory Medicine, Center for Diagnostic Oncology, National Cancer Center, Gyeonggi-do, Korea.
authors.forename: Sang-Hyun
authors.initials: SH
authors.lastname: Hwang
authors.affiliation: MG MED, Inc., Seoul, Korea.
authors.forename: Ho-Young
authors.initials: HY
authors.lastname: Kang