title: Characterization of a TnMAVS protein from Tetraodon nigroviridis.
abstract: A growing family of cellular proteins encoding for caspase activation and the recruitment domain (CARD) plays a crucial role in immunity by sensing viral infections and signaling antiviral immune defenses. We obtained a MAVS-like protein (named TnMAVS) from Tetradon nigroviridis, which contains a CARD domain, a pro-rich domain, and a TM domain similar to human MAVS. A fluorescence assay showed that TnMAVS was located in the cytoplasm and near by the membrane, and not the mitochondria in FHM cells. As such, it was considered as a new member of MAVS. The TnMAVS was highly expressed in the liver and muscle of T. nigroviridis. In the spleen, TnMAVS was down-regulated when the fish was treated with polyinosinic:polycytidylic acid or challenged with ISKNV, but was not affected by PGN or LPS. The dual luciferase reporter assay revealed that TnMAVS overexpression resulted in the activation of the interferon-sensitive response element and NF-κB signal pathways. In addition, a characteristic TRAF3-associated peptide PVQD was found in the TnMAVS sequence. Co-immunoprecipitation assays indicated that TnMAVS could interact with zfTRAF3 in eukaryotic cells.
authors.affiliation: State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou, 510275, PR China.
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Xiang
authors.forename: Lin
authors.initials: L
authors.lastname: Qi
authors.forename: Weijian
authors.initials: W
authors.lastname: Chen
authors.forename: Chuanfu
authors.initials: C
authors.lastname: Dong
authors.forename: Zhaoyu
authors.initials: Z
authors.lastname: Liu
authors.forename: Dong
authors.initials: D
authors.lastname: Liu
authors.forename: Mengli
authors.initials: M
authors.lastname: Huang
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Gan
authors.initials: G
authors.lastname: Yang
authors.forename: Shaoping
authors.initials: S
authors.lastname: Weng
authors.forename: Jianguo
authors.initials: J
authors.lastname: He
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D053468:CARD Signaling Adaptor Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D003593:Cytoplasm; D004266:DNA Virus Infections / Q000276:immunology*; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D017930:Genes, Reporter; D057809:HEK293 Cells; D006801:Humans; D007113:Immunity, Innate; D007372:Interferons / Q000378:metabolism; D007497:Iridoviridae / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000276:immunology / Q000378:metabolism; D011070:Poly I-C / Q000276:immunology; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein; D048008:TNF Receptor-Associated Factor 3 / Q000276:immunology / Q000378:metabolism; D023721:Tetraodontiformes / Q000276:immunology / Q000378:metabolism / Q000821:virology
chemical_list: D053468:CARD Signaling Adaptor Proteins; D029941:Fish Proteins; D008070:Lipopolysaccharides; D016328:NF-kappa B; D048008:TNF Receptor-Associated Factor 3; D007372:Interferons; D011070:Poly I-C