title: hHGF overexpression in myoblast sheets enhances their angiogenic potential in rat chronic heart failure.
abstract: After severe myocardial infarction (MI), heart failure results from ischemia, fibrosis, and remodeling. A promising therapy to enhance cardiac function and induce therapeutic angiogenesis via a paracrine mechanism in MI is myoblast sheet transplantation. We hypothesized that in a rat model of MI-induced chronic heart failure, this therapy could be further improved by overexpression of the antiapoptotic, antifibrotic, and proangiogenic hepatocyte growth factor (HGF) in the myoblast sheets. We studied the ability of wild type (L6-WT) and human HGF-expressing (L6-HGF) L6 myoblast sheet-derived paracrine factors to stimulate cardiomyocyte, endothelial cell, or smooth muscle cell migration in culture. Further, we studied the autocrine effect of hHGF-expression on myoblast gene expression profiles by use of microarray analysis. We induced MI in Wistar rats by left anterior descending coronary artery (LAD) ligation and allowed heart failure to develop for 4 weeks. Thereafter, we administered L6-WT (n = 15) or L6-HGF (n = 16) myoblast sheet therapy. Control rats (n = 13) underwent LAD ligation and rethoracotomy without therapy, and five rats underwent a sham operation in both surgeries. We evaluated cardiac function with echocardiography at 2 and 4 weeks after therapy, and analyzed cardiac angiogenesis and left ventricular architecture from histological sections at 4 weeks. Paracrine mediators from L6-HGF myoblast sheets effectively induced migration of cardiac endothelial and smooth muscle cells but not cardiomyocytes. Microarray data revealed that hHGF-expression modulated myoblast gene expression. In vivo, L6-HGF sheet therapy effectively stimulated angiogenesis in the infarcted and non-infarcted areas. Both L6-WT and L6-HGF therapies enhanced cardiac function and inhibited remodeling in a similar fashion. In conclusion, L6-HGF therapy effectively induced angiogenesis in the chronically failing heart. Cardiac function, however, was not further enhanced by hHGF expression.
authors.affiliation: Institute of Biomedicine, University of Helsinki, Helsinki, Finland. antti.siltanen@helsinki.fi
authors.forename: Antti
authors.initials: A
authors.lastname: Siltanen
authors.forename: Katsukiyo
authors.initials: K
authors.lastname: Kitabayashi
authors.forename: Päivi
authors.initials: P
authors.lastname: Lakkisto
authors.forename: Johanna
authors.initials: J
authors.lastname: Mäkelä
authors.forename: Tommi
authors.initials: T
authors.lastname: Pätilä
authors.forename: Masamichi
authors.initials: M
authors.lastname: Ono
authors.forename: Ilkka
authors.initials: I
authors.lastname: Tikkanen
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Sawa
authors.forename: Esko
authors.initials: E
authors.lastname: Kankuri
authors.forename: Ari
authors.initials: A
authors.lastname: Harjula
mesh_terms: D000818:Animals; D017209:Apoptosis; D001681:Biological Assay; D002460:Cell Line; D002908:Chronic Disease; D005355:Fibrosis; D005786:Gene Expression Regulation; D006333:Heart Failure / Q000000981:diagnostic imaging / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D006334:Heart Function Tests; D017228:Hepatocyte Growth Factor / Q000235:genetics* / Q000627:therapeutic use*; D006801:Humans; D032446:Myoblasts / Q000378:metabolism* / Q000473:pathology; D018919:Neovascularization, Physiologic; D051381:Rats; D017208:Rats, Wistar; D014162:Transfection; D014463:Ultrasonography; D014664:Vasodilation / Q000502:physiology
chemical_list: C066855:HGF protein, human; D017228:Hepatocyte Growth Factor