title: Seminal molecular markers as a non-invasive diagnostic tool for the evaluation of spermatogenesis in non-obstructive azoospermia.
abstract: Non-obstructive azoospermia (NOA) is currently evaluated by the use of conventional histopathological methods. In some cases, focal spermatogenesis is present in the testes of patients with NOA which may be almost undetectable by routine histopathological examinations. Application of molecular markers in semen to predict the spermatogenesis status in the testis will emphasize the probability of finding sperm in NOA testis through further search using TESE or mTESE. Detection of germ cell-specific transcripts in semen is a signal of germ cells present in the testis. In this study, we used molecular methods to evaluate spermatogenesis status in azoospermic men. Semen samples were collected from 203 men with azoospermia. Total RNA was extracted from the semen precipitates. First-strand complementary deoxyribonucleic acid (cDNA) was synthesized by reverse transcriptase then, (RT)-PCRs were carried out using primers for testis stage-specific genes (DAZ, AKAP4, PRM1, and PRM2). Testicular tissue biopsies were used for evaluating spermatogenesis status in testis. Histopathological examination and LH, FSH, and testosterone level measurements (chemiluminescence assay) were performed. The presence of DAZ and PRM2 transcripts in semen significantly indicated the presence of spermatogonia and spermatids in the testicular tissues. Absence of all four markers in semen confirmed the histopathological results corresponding to sertoli cell only syndrome (SCO). Although TESE should not be excluded solely on this criteria, using PRM1, PRM2, AKAP4, and DAZ transcripts in semen would provide a non-invasive molecular diagnostic tool to better counsel patients before undergoing TESE.
authors.affiliation: Department of Anatomy and Cell Biology, Justus-Liebig-University of Giessen, Giessen, Germany.
authors.forename: Ferial
authors.initials: F
authors.lastname: Aslani
authors.forename: Mohammad Hossein
authors.initials: MH
authors.lastname: Modarresi
authors.forename: Haleh
authors.initials: H
authors.lastname: Soltanghoraee
authors.forename: Mohammad Mehdi
authors.initials: MM
authors.lastname: Akhondi
authors.forename: Ashraf
authors.initials: A
authors.lastname: Shabani
authors.forename: Niknam
authors.initials: N
authors.lastname: Lakpour
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Sadeghi
mesh_terms: D054758:A Kinase Anchor Proteins / Q000032:analysis; D000328:Adult; D053713:Azoospermia / Q000175:diagnosis* / Q000503:physiopathology; D015415:Biomarkers / Q000032:analysis*; D000075364:Deleted in Azoospermia 1 Protein; D006801:Humans; D008297:Male; D008875:Middle Aged; D011479:Protamines / Q000032:analysis; D016601:RNA-Binding Proteins / Q000032:analysis; D012661:Semen / Q000378:metabolism*; D013091:Spermatogenesis / Q000502:physiology*; D013737:Testis / Q000473:pathology / Q000503:physiopathology
chemical_list: D054758:A Kinase Anchor Proteins; C485480:AKAP4 protein, human; D015415:Biomarkers; C095668:DAZ1 protein, human; D000075364:Deleted in Azoospermia 1 Protein; C082073:PRM1 protein, human; D011479:Protamines; D016601:RNA-Binding Proteins; C068621:protamine 2