title: Linkage of a RAPD marker with powdery mildew resistance.
abstract: The aim of this study was to investigate the inheritance of powdery mildew disease and to tag it with a DNA marker to utilize for the marker-assisted selection (MAS) breeding program. The powdery mildew resistant genotype Fallon(er) and susceptible genotype 11760-3ER were selected from 177 genotypes by heavy infestation of germplasm with Erysiphe pisi through artificial inoculation. The F1 plants of the cross Fallon/11760-3 indicated the dominance of the susceptible allele, while F2 plants segregated in 3 : 1 ratio (susceptible : resistant) that fit for goodness of fitness by chi2 (P > 0.07), indicating monogenic recessive inheritance for powdery mildew resistance in Pisum sativum. A novel RAPD marker OPB18 (5'-CCACAGCAGT-3') was linked to the er-1 gene with 83% probability with a LOD score of 4.13, and was located at a distance of 11.2 cM from the er-1 gene.
authors.affiliation: Nuasheen University of Malakand, Department of Botany, Khyber Pakhtunkhwa, Pakistan. mnshaalpk@yahoo.com
authors.forename: M
authors.initials: M
authors.lastname: Nisar
authors.forename: A
authors.initials: A
authors.lastname: Ghafoor
mesh_terms: D000704:Analysis of Variance; D001203:Ascomycota; D008040:Genetic Linkage; D005819:Genetic Markers / Q000235:genetics; D005838:Genotype; D018532:Pisum sativum / Q000235:genetics* / Q000382:microbiology*; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology*; D019105:Random Amplified Polymorphic DNA Technique
chemical_list: D005819:Genetic Markers