title: Production of phloroglucinol by Escherichia coli using a stationary-phase promoter.
abstract: Escherichia coli was metabolically engineered using a new host-vector system to produce phloroglucinol. The key biosynthetic gene phlD (encoding a type III polyketide synthase) from Pseudomonas fluorescens was expressed in E. coli using the stationary-phase promoter of the fic gene and a high-copy plasmid. In shake-flasks, the engineered strain produced phloroglucinol up to 0.28 g/l with a productivity of 0.014 g/l h. About 9.2% of the glucose consumed was converted to phloroglucinol after 20 h. Compared with the widely used inducible T7 promoter system, this strain did not require IPTG induction and the final titer of phloroglucinol was 22% higher.
authors.affiliation: Key Lab of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China.
authors.forename: Yujin
authors.initials: Y
authors.lastname: Cao
authors.forename: Mo
authors.initials: M
authors.lastname: Xian
mesh_terms: D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D015870:Gene Expression; D015964:Gene Expression Regulation, Bacterial; D060847:Metabolic Engineering / Q000379:methods*; D053858:Metabolic Networks and Pathways / Q000235:genetics; D010696:Phloroglucinol / Q000378:metabolism*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011551:Pseudomonas fluorescens / Q000201:enzymology / Q000235:genetics; D011995:Recombination, Genetic
chemical_list: D010696:Phloroglucinol