title: Two-photon absorption properties of fluorescent proteins.
abstract: Two-photon excitation of fluorescent proteins is an attractive approach for imaging living systems. Today researchers are eager to know which proteins are the brightest and what the best excitation wavelengths are. Here we review the two-photon absorption properties of a wide variety of fluorescent proteins, including new far-red variants, to produce a comprehensive guide to choosing the right fluorescent protein and excitation wavelength for two-photon applications.
authors.affiliation: Department of Physics, Montana State University, Bozeman, Montana, USA. drobizhev@physics.montana.edu
authors.forename: Mikhail
authors.initials: M
authors.lastname: Drobizhev
authors.forename: Nikolay S
authors.initials: NS
authors.lastname: Makarov
authors.forename: Shane E
authors.initials: SE
authors.lastname: Tillo
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Hughes
authors.forename: Aleksander
authors.initials: A
authors.lastname: Rebane
mesh_terms: D007834:Lasers; D008164:Luminescent Proteins / Q000737:chemistry* / Q000235:genetics; D018613:Microscopy, Confocal / Q000379:methods; D017785:Photons; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D013053:Spectrophotometry / Q000379:methods*; D055672:Static Electricity
chemical_list: D008164:Luminescent Proteins; D011994:Recombinant Proteins