title: Cloning and sequence analysis of IL-2, IL-4 and IFN-γ from Indian Dromedary camels (Camelus dromedarius).
abstract: The cDNAs of three cytokines, viz., IL-2, IL-4 and IFN-γ from Dromedary camels were amplified by PCR using Bactrian camel sequences and subsequently cloned for sequence analysis. Relationship based on amino acid sequences revealed that Dromedary camel IL-2 shared 99.5% and 99.3% identity at the nucleotide and amino acid levels with Bactrian camel IL-2. In the case of IL-4, the identity of Dromedary camel was 99.7% and 99.2% at the nucleotide and amino acid levels, respectively with that of Bactrian camel. The Dromedary camel IFN-γ shared 100% identity both at nucleotide and amino acid levels with Bactrian camel IFN-γ. Phylogenetic analysis based on amino acid sequences indicated the close relationship in these cytokine genes between the Dromedary camel and other camelids.
authors.affiliation: National Research Centre on Camel, Post Bag No. 7, Jorbeer, Bikaner 334 001, Rajasthan, India. camelnag@yahoo.com
authors.forename: G
authors.initials: G
authors.lastname: Nagarajan
authors.forename: Shelesh Kumar
authors.initials: SK
authors.lastname: Swami
authors.forename: S K
authors.initials: SK
authors.lastname: Ghorui
authors.forename: K M L
authors.initials: KM
authors.lastname: Pathak
authors.forename: R K
authors.initials: RK
authors.lastname: Singh
authors.forename: N V
authors.initials: NV
authors.lastname: Patil
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002162:Camelus / Q000235:genetics / Q000276:immunology; D003001:Cloning, Molecular; D007371:Interferon-gamma / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D007376:Interleukin-2 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015847:Interleukin-4 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny
chemical_list: D007376:Interleukin-2; D015847:Interleukin-4; D007371:Interferon-gamma