title: Arginine methylation of the RGG box does not appear to regulate ICP27 import during herpes simplex virus infection.
abstract: Arginine methylation can regulate protein import and export and can modulate protein interactions. Herpes simplex virus 1 (HSV-1) ICP27 is a shuttling protein involved in viral mRNA export. We previously reported that ICP27 is methylated on three arginines within its RGG box and that arginine methylation regulates ICP27 export and its interaction with SRPK1 and Aly/REF. Here, we report that ICP27 was efficiently imported into the nucleus when hypomethylated as determined by Fluorescence Recovery After Photobleaching (FRAP). Furthermore, coimmunoprecipitation of ICP27 with β-importin was not significantly affected by ICP27 hypomethylation. Thus, ICP27 import does not appear to be regulated by arginine methylation.
authors.affiliation: Department of Microbiology and Molecular Genetics, School of Medicine, Medical Sciences B240, University of California, Irvine, CA 92697-4025, USA.
authors.forename: Stuart K
authors.initials: SK
authors.lastname: Souki
authors.forename: Felicia P
authors.initials: FP
authors.lastname: Hernandez
authors.forename: Rozanne M
authors.initials: RM
authors.lastname: Sandri-Goldin
mesh_terms: D001120:Arginine / Q000235:genetics / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism*; D004279:DNA, Viral / Q000235:genetics; D036681:Fluorescence Recovery After Photobleaching / Q000379:methods; D015967:Gene Expression Regulation, Viral; D006367:HeLa Cells / Q000821:virology; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D006801:Humans; D017874:Immediate-Early Proteins / Q000378:metabolism*; D008745:Methylation; D021381:Protein Transport
chemical_list: D004279:DNA, Viral; C057120:ICP27 protein, human herpesvirus 1; D017874:Immediate-Early Proteins; D001120:Arginine