title: Laser-induced thrombosis in zebrafish.
abstract: In the event of injury to the vasculature in vertebrate organisms bleeding is stopped by a defense mechanism called hemostasis. Even though biochemical studies characterized a number of factors, classical genetic methods have not been applied to study hemostasis. We introduced zebrafish as an animal model to study genetics of hemostasis. To conduct genetic studies of hemostasis, we required a global screening method to address all the factors of hemostasis such as those present in plasma, in platelets or those present in the endothelium. Therefore, we developed a global laser induced thrombosis method which can assay all these components. In this paper, we describe the principle of this method as well as provide the detailed protocol so this could be used as a screening tool to measure hemostasis in any laboratory.
authors.affiliation: Department of Biological Sciences, University of North Texas, Denton, Texas, USA.
authors.forename: Pudur
authors.initials: P
authors.lastname: Jagadeeswaran
authors.forename: Maira
authors.initials: M
authors.lastname: Carrillo
authors.forename: Uvaraj P
authors.initials: UP
authors.lastname: Radhakrishnan
authors.forename: Surendra K
authors.initials: SK
authors.lastname: Rajpurohit
authors.forename: Seongcheol
authors.initials: S
authors.lastname: Kim
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D006487:Hemostasis; D006801:Humans; D007834:Lasers; D013927:Thrombosis / Q000097:blood / Q000209:etiology*; D015027:Zebrafish / Q000097:blood*