title: Characterization of sarcoplasmic calcium binding protein (SCP) variants from freshwater crayfish Procambarus clarkii.
abstract: Sarcoplasmic calcium binding protein (SCP) is an invertebrate EF-hand calcium buffering protein that has been proposed to fulfill a similar function in muscle relaxation as vertebrate parvalbumin. We have identified three SCP variants in the freshwater crayfish Procambarus clarkii. The variants (pcSCP1a, pcSCP1b, and pcSCP1c) differ across a 37 amino acid region that lies mainly between the second and third EF-hand calcium binding domains. We evaluated tissue distribution and response of the variants to cold exposure, a stress known to affect expression of parvalbumin. Expression patterns of the variants were not different and therefore do not provide a functional rationale for the polymorphism of pcSCP1. Compared to hepatopancreas, expression of pcSCP1 variants was 100,000-fold greater in axial abdominal muscle and 10-fold greater in cardiac muscle. Expression was 10-100 greater in fast-twitch deep flexor and extensor muscles compared to slow-twitch superficial flexor and extensors. In axial muscle, no significant changes of pcSCP1, calmodulin (CaM), or sarcoplasmic/endoplasmic reticulum Ca-ATPase (SERCA) expression were measured after one week of 4°C exposure. In contrast, large decreases of pcSCP1 were measured in cardiac muscle, with no changes in CaM or SERCA. Knockdown of pcSCP1 by dsRNA led to reduced muscle activity and decreased expression of SERCA. In summary, the pattern of pcSCP1 tissue expression is similar to parvalbumin, supporting a role in muscle contraction. However, the response of pcSCP1 to cold exposure differs from parvalbumin, suggesting possible functional divergence between the two proteins.
authors.affiliation: Department of Biology, Kenyon College, Gambier, OH 43022, USA.
authors.forename: Alexandra J
authors.initials: AJ
authors.lastname: White
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Northcutt
authors.forename: Suzanne E
authors.initials: SE
authors.lastname: Rohrback
authors.forename: Robert O
authors.initials: RO
authors.lastname: Carpenter
authors.forename: Margaret M
authors.initials: MM
authors.lastname: Niehaus-Sauter
authors.forename: Yongping
authors.initials: Y
authors.lastname: Gao
authors.forename: Michele G
authors.initials: MG
authors.lastname: Wheatly
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Gillen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003400:Astacoidea / Q000235:genetics / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D003080:Cold Temperature; D005260:Female; D008297:Male; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D012519:Sarcoplasmic Reticulum / Q000378:metabolism*; D016415:Sequence Alignment; D014018:Tissue Distribution
chemical_list: D002135:Calcium-Binding Proteins; D020033:Protein Isoforms; D002118:Calcium