title: A novel disposable electrochemical immunosensor for phenyl urea herbicide diuron.
abstract: A disposable electrochemical immunosensor has been developed for the determination of phenyl urea herbicide-diuron using a low cost laser ablated gold electrodes (LC-LAGE) fabricated on polystyrene substrate. The electrodes were electrochemically deposited with prussian blue-gold nanoparticle (PB-GNP) film, and a competitive inhibition immunoassay was performed on LC-LAGE by using a specific hapten-protein conjugate. The binding of available diuron specific antibody on conjugate coated electrode was detected using alkaline phosphatase rabbit anti-IgG antibody. The addition of 1-naphthyl phosphate substrate resulted in the production of electrochemically active product, 1-naphthol, which was monitored using square wave voltammetry technique. The assay exhibited an excellent sensitivity and specificity showing the dynamic response range between 1 ppt and 10 ppm for diuron with detection limit around 1 ppt. This study provides insight into development of a rapid and high-throughput screening of pesticides in environmental samples at a very low cost.
authors.affiliation: Institute of Microbial Technology (CSIR), Sector 39-A, Chandigarh 160036, India.
authors.forename: Priyanka
authors.initials: P
authors.lastname: Sharma
authors.forename: Kavita
authors.initials: K
authors.lastname: Sablok
authors.forename: Vijayender
authors.initials: V
authors.lastname: Bhalla
authors.forename: C Raman
authors.initials: CR
authors.lastname: Suri
mesh_terms: D000818:Animals; D055910:Antibodies, Immobilized; D015374:Biosensing Techniques / Q000379:methods* / Q000706:statistics & numerical data; D004237:Diuron / Q000032:analysis* / Q000276:immunology; D055664:Electrochemical Techniques; D004784:Environmental Monitoring / Q000379:methods; D006046:Gold; D006241:Haptens; D006540:Herbicides / Q000032:analysis* / Q000276:immunology; D007118:Immunoassay / Q000379:methods / Q000706:statistics & numerical data; D053768:Metal Nanoparticles; D011817:Rabbits; D012680:Sensitivity and Specificity
chemical_list: D055910:Antibodies, Immobilized; D006241:Haptens; D006540:Herbicides; D006046:Gold; D004237:Diuron