title: Microcirculation and metastasis in a new mouse mammary tumor model system.
abstract: Two new metastatic mouse mammary tumor transplant lines have been established in nude mice. The Met-1 line, with the polyoma virus middle T (PyV-MT) transgene, metastasized with 100% efficiency. The Db-7 line, expressing a PyV-MT transgene mutated at positions 315 and 322, metastasized with 8.8% efficiency. Histology and computer-assisted intravital microscopy demonstrated that internal microcirculation in Met-1 was more complex than Db-7; Met-1 exhibited higher microvessel density and tortuosity (P < 0.0001). These indices of microvascular complexity correlated with the higher Met-1 metastatic rate (P < 0.0001). These two transplantable lines will be useful for investigating the complex relationship between angiogenesis and metastasis.
authors.affiliation: UNIV CALIF DAVIS,SCH MED,DEPT MED PATHOL,DAVIS,CA 95616. UNIV CALIF SAN DIEGO,INST BIOMED ENGN,LA JOLLA,CA 92093. FOURTH MIL MED UNIV,TEACHING HOSP 2,DEPT PATHOL,XIAN 710038,PEOPLES R CHINA. MCMASTER UNIV,INST MOL BIOL & TECHNOL,HAMILTON,ON L8S 4K1,CANADA.
authors.forename: A
authors.initials: A
authors.lastname: Cheung
authors.forename: L
authors.initials: L
authors.lastname: Young
authors.forename: P
authors.initials: P
authors.lastname: Chen
authors.forename: C
authors.initials: C
authors.lastname: Chao
authors.forename: A
authors.initials: A
authors.lastname: Ndoye
authors.forename: P
authors.initials: P
authors.lastname: Barry
authors.forename: W
authors.initials: W
authors.lastname: Muller
authors.forename: R
authors.initials: R
authors.lastname: Cardiff