title: The anti-apoptotic Bcl-x(L) protein, a new piece in the puzzle of cytochrome c interactome.
abstract: A structural model of the adduct between human cytochrome c and the human anti-apoptotic protein Bcl-x(L), which defines the protein-protein interaction surface, was obtained from solution NMR chemical shift perturbation data. The atomic level information reveals key intermolecular contacts identifying new potentially druggable areas on cytochrome c and Bcl-x(L). Involvement of residues on cytochrome c other than those in its complexes with electron transfer partners is apparent. Key differences in the contact area also exist between the Bcl-x(L) adduct with the Bak peptide and that with cytochrome c. The present model provides insights to the mechanism by which cytochrome c translocated to cytosol can be intercepted, so that the apoptosome is not assembled.
authors.affiliation: Magnetic Resonance Center (CERM), University of Florence, Sesto Fiorentino, Florence, Italy. bertini@cerm.unifi.it
authors.forename: Ivano
authors.initials: I
authors.lastname: Bertini
authors.forename: Soizic
authors.initials: S
authors.lastname: Chevance
authors.forename: Rebecca
authors.initials: R
authors.lastname: Del Conte
authors.forename: Daniela
authors.initials: D
authors.lastname: Lalli
authors.forename: Paola
authors.initials: P
authors.lastname: Turano
mesh_terms: D017209:Apoptosis; D045304:Cytochromes c / Q000737:chemistry / Q000378:metabolism*; D004579:Electron Transport; D006801:Humans; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular; D011485:Protein Binding; D055672:Static Electricity; D051020:bcl-X Protein / Q000737:chemistry / Q000378:metabolism*
chemical_list: D051020:bcl-X Protein; D045304:Cytochromes c