title: [Sequencing and analysis of the complete genome sequence of WU polyomavirus in Fuzhou, China].
abstract: WU polyomavirus (WUPyV), a new member of the genus Polyomavirus in the family Polyomaviridae, is recently found in patients with respiratory tract infections. In our study, the complete genome of the two WUPyV isolates (FZ18, FZTF) were sequenced and deposited in GenBank (accession nos. FJ890981, FJ890982). The two sequences of the WUPyV isolates in this study varied little from each other. Compared with other complete genome sequences of WUPyV in GenBank (strain B0, S1-S4, CLFF, accession nos. EF444549, EF444550, EF444551, EF444552, EF444553, EU296475 respectively), the sequence length in nucleotides is 5228bp, 1bp shorter than the known sequences. The deleted base pair was at nucleotide position 4536 in the non-coding region of large T antigen (LTAg). The genome of the WUPyV encoded for five proteins. They were three capsid proteins: VP2, VP1, VP3 and LTAg, small T antigen (STAg), respectively. To investigate whether these nucleotide sequences had any unique features, we compared the genome sequence of the 2 WUPyV isolates in Fuzhou, China to those documented in the GenBank database by using PHYLIP software version 3.65 and the neighbor-joining method. The 2 WUPyV strains in our study were clustered together. Strain FZTF was more closed to the reference strain B0 of Australian than strain FZ18.
authors.affiliation: Fujian Center for Disease Control and Prevention, Fuzhou 350001, China. wqshiu@live.cn
authors.forename: Wen-qiong
authors.initials: WQ
authors.lastname: Xiu
authors.forename: Xiao-na
authors.initials: XN
authors.lastname: Shen
authors.forename: Guang-hua
authors.initials: GH
authors.lastname: Liu
authors.forename: Jian-feng
authors.initials: JF
authors.lastname: Xie
authors.forename: Yu-lan
authors.initials: YL
authors.lastname: Kang
authors.forename: Mei-ai
authors.initials: MA
authors.lastname: Wang
authors.forename: Wen-qing
authors.initials: WQ
authors.lastname: Zhang
authors.forename: Qi-zhu
authors.initials: QZ
authors.lastname: Weng
authors.forename: Yan-sheng
authors.initials: YS
authors.lastname: Yan
mesh_terms: D000328:Adult; D002675:Child, Preschool; D002681:China; D019143:Evolution, Molecular; D016679:Genome, Viral / Q000235:genetics*; D023281:Genomics; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D027382:Polyomaviridae / Q000235:genetics* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA / Q000379:methods*