title: Goose parvovirus structural proteins expressed by recombinant baculoviruses self-assemble into virus-like particles with strong immunogenicity in goose.
abstract: Goose parvovirus (GPV), a small non-enveloped ssDNA virus, can cause Derzsy's disease, and three capsid proteins of VP1, VP2, and VP3 are encoded by an overlapping nucleotide sequence. However, little is known on whether recombinant viral proteins (VPs) could spontaneously assemble into virus-like particles (VLPs) in insect cells and whether these VLPs could retain their immunoreactivity and immunogenicity in susceptible geese. To address these issues, genes for these GPV VPs were amplified by PCR, and the recombinant VPs proteins were expressed in insect cells using a baculovirus expression system for the characterization of their structures, immunoreactivity, and immunogenicity. The rVP1, rVP2, and rVP3 expressed in Sf9 cells were detected by anti-GPV sera, anti-VP3 sera, and anti-His antibodies, respectively. Electron microscopy revealed that these rVPs spontaneously assembled into VLPs in insect cells, similar to that of the purified wild-type GPV virions. In addition, vaccination with individual types of VLPs, particularly with the rVP2-VLPs, induced higher titers of antibodies and neutralized different strains of GPVs in primary goose and duck embryo fibroblast cells in vitro. These data indicated that these VLPs retained immunoreactivity and had strong immunogenicity in susceptible geese. Therefore, our findings may provide a framework for development of new vaccines for the prevention of Derzsy's disease and vehicles for the delivery of drugs.
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang, PR China.
authors.forename: Huanyu
authors.initials: H
authors.lastname: Ju
authors.forename: Na
authors.initials: N
authors.lastname: Wei
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.forename: Chunyuan
authors.initials: C
authors.lastname: Wang
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Jing
authors.forename: Lu
authors.initials: L
authors.lastname: Guo
authors.forename: Dapeng
authors.initials: D
authors.lastname: Liu
authors.forename: Mingchun
authors.initials: M
authors.lastname: Gao
authors.forename: Bo
authors.initials: B
authors.lastname: Ma
authors.forename: Junwei
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D016367:Baculoviridae; D036022:Capsid Proteins / Q000235:genetics / Q000276:immunology*; D005777:Geese / Q000276:immunology / Q000821:virology*; D007313:Insecta / Q000166:cytology; D010322:Parvoviridae Infections / Q000517:prevention & control / Q000662:veterinary*; D017992:Parvovirus / Q000276:immunology*; D011201:Poultry Diseases / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology*; D014771:Virion / Q000235:genetics / Q000276:immunology*; D019065:Virus Assembly
chemical_list: D036022:Capsid Proteins; D011994:Recombinant Proteins; D014765:Viral Vaccines