title: [The cellular and molecular mechanism of inhibitory effect of asiaticoside on capsular contracture following breast augmentation].
abstract: OBJECTIVE
  To explore the cellular and molecular mechanism of the inhibitory effect of asiaticoside on capsular contracture following breast augmentation.
  
  
  METHODS
  Contracture capsule derived fibroblasts were cultured in medium with different concentration of asiaticoside. The cell proliferation, collage synthesis and alpha-SMA expression were detected by means of 3H-thymidine incorporation, 3H-proline incorporation, and Western-blot. The results were analyzed by SPSS 11.0 with t test.
  
  
  RESULTS
  DNA and collagen synthesis of fibroblasts were dramatically inhibited when the asiaticoside reached the concentration of 50 mg/L. The inhibitory rate was 34.7% and 30.1% respectively, showing a significant difference from that in control group (P < 0.05). The inhibitory effect increased with the rise of the asiaticoside concentration in a dose-dependent manner. When the concentration of asiaticoside reached 25 mg/L, the expression of alpha-SMA was down-regulated with an activation index of 1.673, showing a significant difference when compared with that in control group (P < 0.05).
  
  
  CONCLUSIONS
  asiaticoside can effectively inhibit the DNA and collagen synthesis of capsule-derived fibroblasts. The trans-differentiation of fibroblast to myo-fibroblasts is also prevented by it.
authors.affiliation: Department of Plastic and Aesthetic Surgery, Plastic and Aesthetic Surgery, Southwest Hospital, the Third Military Medical University, Chongqing 400038, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Dai
authors.forename: Zhe
authors.initials: Z
authors.lastname: Li
authors.forename: Jian-yi
authors.initials: JY
authors.lastname: Liu
authors.forename: Shi-rong
authors.initials: SR
authors.lastname: Li
authors.forename: Ju-long
authors.initials: JL
authors.lastname: Wu
mesh_terms: D000328:Adult; D001940:Breast / Q000601:surgery; D054338:Cell Transdifferentiation; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis; D003286:Contracture / Q000209:etiology / Q000517:prevention & control*; D004247:DNA / Q000096:biosynthesis; D005260:Female; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D016462:Mammaplasty; D011183:Postoperative Complications / Q000517:prevention & control; D014315:Triterpenes / Q000627:therapeutic use*
chemical_list: D014315:Triterpenes; D003094:Collagen; D004247:DNA; C004446:asiaticoside