title: [Analysis of the genetic constructs expressing antiviral siRNAs in the non-viral test system].
abstract: The efficiency of RNA interference triggered by the genetic constructs encoding siRNAs and directed against human immunodeficiency virus type 1 (HIV-1) transcripts was investigated in the non-viral test system. The investigation showed 6 biologically active siRNAs attacking 6 conservative targets in the HIV-1 genome. Expression of these genetic constructs failed to induce a nonspecific interferon response.
authors.forename: I R
authors.initials: IR
authors.lastname: Alembekov
authors.forename: O V
authors.initials: OV
authors.lastname: Kretova
authors.forename: N A
authors.initials: NA
authors.lastname: Churikov
mesh_terms: D000998:Antiviral Agents; D001681:Biological Assay; D016679:Genome, Viral; D057809:HEK293 Cells; D015497:HIV-1 / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007372:Interferons / Q000378:metabolism*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D012367:RNA, Viral / Q000235:genetics
chemical_list: D000998:Antiviral Agents; D034741:RNA, Small Interfering; D012367:RNA, Viral; D007372:Interferons