title: Synthesis of biotinylated muramyl tripeptides with NOD2-stimulating activity.
abstract: Muramyl di- and tri-peptides are putative activators of the innate immune system through stimulation of the NOD2 receptor. To provide tools for the clarification of the mechanism of this activation we isolated different UDP-muramyl tripeptides (Lys- and DAP-type) from bacteria and used them to synthesize biotinylated derivatives. All biotinylated compounds retained their ability to activate NOD2 in a cell-based test system and are therefore suitable for binding studies aimed at identifying the appropriate pattern recognition receptor(s).
authors.affiliation: Department of Molecular Infection Biology, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Parkallee 1-40, 23845 Borstel, Germany.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Gisch
authors.forename: Birte
authors.initials: B
authors.lastname: Buske
authors.forename: Holger
authors.initials: H
authors.lastname: Heine
authors.forename: Buko
authors.initials: B
authors.lastname: Lindner
authors.forename: Ulrich
authors.initials: U
authors.lastname: Zähringer
mesh_terms: D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D000276:Adjuvants, Immunologic; D019921:Biotinylation; D005786:Gene Expression Regulation / Q000187:drug effects; D057809:HEK293 Cells; D006801:Humans; D007113:Immunity, Innate; D053473:Nod2 Signaling Adaptor Protein / Q000378:metabolism*; D009842:Oligopeptides / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000276:Adjuvants, Immunologic; D053473:Nod2 Signaling Adaptor Protein; D009842:Oligopeptides; D000119:Acetylmuramyl-Alanyl-Isoglutamine; C035104:muramylNAc-Ala-isoGln-Lys-tripeptide