title: Identification of PSA peptide mimotopes using phage display peptide library.
abstract: Prostate cancer (PCa) is one of the most common types of cancer in men in the United States and is the second leading cause of cancer related death in men. Clinically, secreted prostate specific antigen (PSA) has gained recognition because of its proteolytic activity being directly linked to PCa cell proliferation leading to disease initiation and progression. Using phage display technology, we identified four distinct cyclical peptides. These peptides apart from differences in their amino acid sequence, elicited minimal cross reactive antibody responses against each other. One of the four peptides analyzed produced an antibody response that recognizes the PSA protein. We demonstrate that the synthetic PSA peptide mimics identified in our study are immunologically active and produce neutralizing activity and this has relevance and utility for prostate cancer disease progression.
authors.affiliation: Department of Microbiology and Immunology, New York Medical College, Valhalla, New York 10595, USA.
authors.forename: Arulkumaran
authors.initials: A
authors.lastname: Shanmugam
authors.forename: Robert
authors.initials: R
authors.lastname: Suriano
authors.forename: Devyani
authors.initials: D
authors.lastname: Chaudhuri
authors.forename: Shilpi
authors.initials: S
authors.lastname: Rajoria
authors.forename: Andrea
authors.initials: A
authors.lastname: George
authors.forename: Abraham
authors.initials: A
authors.lastname: Mittelman
authors.forename: Raj K
authors.initials: RK
authors.lastname: Tiwari
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D000951:Antigens, Neoplasm / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D015153:Blotting, Western; D019496:Cancer Vaccines / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D045744:Cell Line, Tumor; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018716:Molecular Mimicry; D008969:Molecular Sequence Data; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry* / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D017430:Prostate-Specific Antigen / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000235:genetics / Q000276:immunology* / Q000378:metabolism
chemical_list: D000951:Antigens, Neoplasm; D019496:Cancer Vaccines; D019151:Peptide Library; D010455:Peptides; D017430:Prostate-Specific Antigen