title: H2 inhibits TNF-α-induced lectin-like oxidized LDL receptor-1 expression by inhibiting nuclear factor κB activation in endothelial cells.
abstract: H(2) is a therapeutic antioxidant that can reduce oxidative stress. Oxidized low-density lipoprotein, which plays roles in atherosclerosis, may promote endothelial dysfunction by binding the cell-surface receptor LOX-1. LOX-1 expression can be upregulated by various stimuli, including TNF-α. Thus, we aimed to examine whether the upregulation of LOX-1 by different stimuli could be blocked by H(2) in endothelial cells. H(2) significantly abolished the upregulation of LOX-1 by different stimuli, including TNF-α, at the protein and mRNA levels. The TNF-α-induced upregulation of LOX-1 was also attenuated by the NF-κB inhibitor N-acetyl-L-cysteine. H(2) inhibited the TNF-α-induced activation of NF-κB and the phosphorylation of IκB-α. Furthermore, H(2) inhibited the expression of LOX-1 and the activation of NF-κB in apolipoprotein E knockout mice, an animal model of atherosclerosis. Thus, H(2) probably inhibits cytokine-induced LOX-1 gene expression by suppressing NF-κB activation.
authors.affiliation: Institute of Atherosclerosis, TaiShan Medical University, ShanDong, China. girl_sapphire@hotmail.com
authors.forename: Guohua
authors.initials: G
authors.lastname: Song
authors.forename: Hua
authors.initials: H
authors.lastname: Tian
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
authors.forename: Hongle
authors.initials: H
authors.lastname: Zhang
authors.forename: Xuejun
authors.initials: X
authors.lastname: Sun
authors.forename: Shucun
authors.initials: S
authors.lastname: Qin
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001011:Aorta / Q000473:pathology; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D006859:Hydrogen / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D051131:Receptors, Oxidized LDL / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D016328:NF-kappa B; D012333:RNA, Messenger; D051131:Receptors, Oxidized LDL; D014409:Tumor Necrosis Factor-alpha; D006859:Hydrogen