title: [Investigation of pseudoautosomal and bordering regions in avian Z and W chromosomes with the use of large insert genomic BAC clones].
abstract: To study pseudoautosomal and bordering regions in the avian Z and W chromosomes, we used seven BAC clones from genomic libraries as DNA probes of fragments of different gametologs of the ATP5A1 gene located close to the proximal border of the pseudoautosomal region (PAR) of sex chromosomes of domestic chicken and Japanese quail. Localization of BAC clones TAM31-b100C09, TAM31-b99N01, TAM31-b27P16, and TAM31-b95L18 in the short arm of Z chromosomes of domestic chicken and Japanese quail (region Zp23-p22) and localization of the BAC clones CHORI-261-CH46G16, CHORI-261-CH33F10, and CHORI-261-CH64F22 on W chromosomes of these species and in the short arm of Z chromosomes (region Zp23-p22) were determined by fluorescence in situ hybridization with the use of W-specific probes. The difference in the localization of the BAC clones on the Z and W chromosomes is probably explained by divergence of the nucleotide sequences of different sex chromosomes located beyond the pseudoautosomal region.
authors.forename: I Iu
authors.initials: IIu
authors.lastname: Blagoveshchenskiĭ
authors.forename: A L
authors.initials: AL
authors.lastname: Sazanova
authors.forename: V A
authors.initials: VA
authors.lastname: Stekol'nikova
authors.forename: K A
authors.initials: KA
authors.lastname: Fomichev
authors.forename: O Iu
authors.initials: OIu
authors.lastname: Barkova
authors.forename: M N
authors.initials: MN
authors.lastname: Romanov
authors.forename: A A
authors.initials: AA
authors.lastname: Sazanov
mesh_terms: D000818:Animals; D001717:Birds / Q000235:genetics*; D002645:Chickens / Q000235:genetics; D022202:Chromosomes, Artificial, Bacterial; D003370:Coturnix / Q000235:genetics; D015698:Genomic Library; D017404:In Situ Hybridization, Fluorescence; D011995:Recombination, Genetic; D012730:Sex Chromosomes / Q000235:genetics*