title: Plant gateway vectors for RNAi as a tool for functional genomic studies.
abstract: Gateway(®) system takes advantage of high-throughput creation of various expression vectors from one entry vector. This technology is also applied to RNAi vectors for functional analysis of plant genomics. To date, several plant Gateway vectors have been developed and distributed to plant science community. Here I would like to introduce unique plant Gateway vectors developed for functional analysis of the metabolic pathway in root tissues. The protocol shown here is basically applied to other plant Gateway vectors for RNAi.
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan. muranaka@bio.eng.osaka-u.ac.jp
authors.forename: Toshiya
authors.initials: T
authors.lastname: Muranaka
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D018274:Electroporation; D023061:Gene Order; D005822:Genetic Vectors / Q000235:genetics* / Q000378:metabolism*; D023281:Genomics; D010944:Plants / Q000235:genetics* / Q000378:metabolism*; D034622:RNA Interference; D011995:Recombination, Genetic / Q000235:genetics; D014169:Transformation, Bacterial