title: Head and neck carconogenesis: impact of MTHFD1 G1958A polymorphism.
abstract: OBJECTIVE
  To investigate the MTHFD1 G1958A polymorphism involved in the folate metabolism as a risk for head and neck cancer, and to find the association of the polymorphism with the risk factors and clinical and histopathological characteristics.
  
  
  METHODS
  Retrospective study investigating MTHFD1 G1958A polymorphism in 694 subjects (240 patients in the Case Group and 454 in the Control Group) by Restriction Fragment Length Polymorphism (RFLP) Analysis. Multiple logistic regression and chi-square tests were used in the statistical analysis.
  
  
  RESULTS
  Multivariable analysis showed that smoking and age over 42 years were disease predictors (p < 0.05). MTHFD1 1958GA or AA genotypes were associated with smoking (p = 0.04) and alcoholism (p = 0.03) and were more often found in more advanced stage tumors (p = 0.04) and in patients with a shorter survival (p = 0.03).
  
  
  CONCLUSIONS
  The presence of MTHFD1 G1948A polymorphism associated with smoking and alcoholism raises the head and neck cancer risk.
authors.affiliation: Unit of Research in Genetics and Molecular Biology (UPGEM), Medical College of São José do Rio Preto (FAMERP), São José do Rio Preto, SP, Brazil. eny.goloni@famerp.br
authors.forename: Lidia Maria Rebolho Batista da
authors.initials: LM
authors.lastname: Silva
authors.forename: Jéssika Nunes Gomes da
authors.initials: JN
authors.lastname: Silva
authors.forename: Ana Lívia Silva
authors.initials: AL
authors.lastname: Galbiatti
authors.forename: Maysa
authors.initials: M
authors.lastname: Succi
authors.forename: Mariangela Torreglosa
authors.initials: MT
authors.lastname: Ruiz
authors.forename: Luiz Sérgio
authors.initials: LS
authors.lastname: Raposo
authors.forename: José Víctor
authors.initials: JV
authors.lastname: Maniglia
authors.forename: Erika Cristina
authors.initials: EC
authors.lastname: Pavarino-Bertelli
authors.forename: Eny Maria
authors.initials: EM
authors.lastname: Goloni-Bertollo
mesh_terms: D000328:Adult; D000367:Age Factors; D000428:Alcohol Drinking / Q000009:adverse effects; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006258:Head and Neck Neoplasms / Q000201:enzymology / Q000235:genetics*; D006801:Humans; D008297:Male; D008754:Methylenetetrahydrofolate Dehydrogenase (NADP) / Q000235:genetics*; D008875:Middle Aged; D015778:Minor Histocompatibility Antigens; D009363:Neoplasm Proteins / Q000235:genetics*; D011110:Polymorphism, Genetic; D012189:Retrospective Studies; D012307:Risk Factors; D012907:Smoking / Q000009:adverse effects
chemical_list: D015778:Minor Histocompatibility Antigens; D009363:Neoplasm Proteins; C539735:MTHFD1 protein, human; D008754:Methylenetetrahydrofolate Dehydrogenase (NADP)