title: Enzyme-responsive multifunctional magnetic nanoparticles for tumor intracellular drug delivery and imaging.
abstract: Enzyme-responsive, hybrid, magnetic silica nanoparticles have been employed for multifunctional applications in selective drug delivery and intracellular tumor imaging. In this study, doxorubicin (Dox)-conjugated, enzyme-cleavable peptide precursors were covalently tethered onto the surface of uniform silica-coated magnetic nanoparticles through click chemistry. This enzyme-responsive nanoparticle conjugate demonstrated highly efficient Dox release upon specific enzyme interactions in vitro. It also exhibits multiple functions in selective tumor intracellular drug delivery and imaging in the tumor cells with high cathepsin B expression, whereas it exhibited lower cytotoxicity towards other cells without enzyme expression.
authors.affiliation: Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore 637371, Singapore.
authors.forename: Yanmei
authors.initials: Y
authors.lastname: Yang
authors.forename: Junxin
authors.initials: J
authors.lastname: Aw
authors.forename: Kai
authors.initials: K
authors.lastname: Chen
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.forename: Parasuraman
authors.initials: P
authors.lastname: Padmanabhan
authors.forename: Yanglong
authors.initials: Y
authors.lastname: Hou
authors.forename: Zhen
authors.initials: Z
authors.lastname: Cheng
authors.forename: Bengang
authors.initials: B
authors.lastname: Xing
mesh_terms: D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage / Q000737:chemistry* / Q000633:toxicity; D002401:Cathepsin B / Q000378:metabolism*; D045744:Cell Line, Tumor; D057930:Click Chemistry; D004317:Doxorubicin / Q000008:administration & dosage / Q000737:chemistry* / Q000633:toxicity; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry*; D005290:Ferric Compounds / Q000737:chemistry; D006801:Humans; D008279:Magnetic Resonance Imaging; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D051379:Mice; D008856:Microscopy, Fluorescence; D009369:Neoplasms / Q000175:diagnosis / Q000188:drug therapy / Q000201:enzymology; D010455:Peptides / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D000903:Antibiotics, Antineoplastic; D004337:Drug Carriers; D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; D010455:Peptides; C000499:ferric oxide; D012822:Silicon Dioxide; D004317:Doxorubicin; D002401:Cathepsin B