title: Effect of post-harvest heat treatment on proteome change of peach fruit during ripening.
abstract: The extracted proteins from the heat-treated peach fruit (dipped in hot water at 48°C for 10min and then stored at room temperature (20°C-25°C) for up to 6 days) were used for proteomic analysis in order to understand the response of post-harvest peach fruit to heat treatment during ripening stage at proteomic level. After two dimensional gels electrophoresis (2-DE) was conducted, more than 600 protein spots were detected. Among them, 35 differently expressed spots (P<0.05) were selected to be excised and analyzed using MALDI-TOF/TOF, and finally 30 protein spots were confidently identified according to NCBI database. The results demonstrated that among the thirty protein spots expressed particularly induced by heat treatment, 43% were related to stress response, 17% to cell structure, 13% to protein fate, 7% to glycolytic pathway, 3% to ripening and senescence and 17% to unclassified. All of them are involved in the regulation of peach fruit development and ripening. All these indicated that the self-defense capability of peach fruit was improved by heat treatment. The study will enable future detailed investigation of gene expression and function linked with peach fruit ripening.
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Zhifang
authors.initials: Z
authors.lastname: Yu
authors.forename: Li
authors.initials: L
authors.lastname: Jiang
authors.forename: Juan
authors.initials: J
authors.lastname: Jiang
authors.forename: Haibo
authors.initials: H
authors.lastname: Luo
authors.forename: Linran
authors.initials: L
authors.lastname: Fu
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional; D005638:Fruit / Q000737:chemistry; D018506:Gene Expression Regulation, Plant; D018869:Heat-Shock Response; D006358:Hot Temperature; D010940:Plant Proteins / Q000032:analysis*; D020543:Proteome / Q000032:analysis*; D027861:Prunus / Q000737:chemistry / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D010940:Plant Proteins; D020543:Proteome