title: Lipase from marine Aspergillus awamori BTMFW032: production, partial purification and application in oil effluent treatment.
abstract: Marine fungus BTMFW032, isolated from seawater and identified as Aspergillus awamori, was observed to produce an extracellular lipase, which could reduce 92% fat and oil content in the effluent laden with oil. In this study, medium for lipase production under submerged fermentation was optimized statistically employing response surface method toward maximal enzyme production. Medium with soyabean meal-0.77% (w/v); (NH(4))(2)SO(4)-0.1m; KH(2)PO(4)-0.05 m; rice bran oil-2% (v/v); CaCl(2)-0.05 m; PEG 6000-0.05% (w/v); NaCl-1% (w/v); inoculum-1% (v/v); pH 3.0; incubation temperature 35°C and incubation period-five days were identified as optimal conditions for maximal lipase production. The time course experiment under optimized condition, after statistical modeling, indicated that enzyme production commenced after 36 hours of incubation and reached a maximum after 96 hours (495.0 U/ml), whereas maximal specific activity of enzyme was recorded at 108 hours (1164.63 U/mg protein). After optimization an overall 4.6-fold increase in lipase production was achieved. Partial purification by (NH(4))(2)SO(4) precipitation and ion exchange chromatography resulted in 33.7% final yield. The lipase was noted to have a molecular mass of 90 kDa and optimal activity at pH 7 and 40°C. Results indicated the scope for potential application of this marine fungal lipase in bioremediation.
authors.affiliation: Microbial Technology Laboratory, Department of Biotechnology, Cochin University of Science & Technology, Cochin 682022, Kerala, India.
authors.forename: Soorej M
authors.initials: SM
authors.lastname: Basheer
authors.forename: Sreeja
authors.initials: S
authors.lastname: Chellappan
authors.forename: P S
authors.initials: PS
authors.lastname: Beena
authors.forename: Rajeev K
authors.initials: RK
authors.lastname: Sukumaran
authors.forename: K K
authors.initials: KK
authors.lastname: Elyas
authors.forename: M
authors.initials: M
authors.lastname: Chandrasekaran
mesh_terms: D001230:Aspergillus / Q000201:enzymology* / Q000254:growth & development*; D001673:Biodegradation, Environmental; D002852:Chromatography, Ion Exchange; D003470:Culture Media; D005590:Fractional Precipitation; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification; D008049:Lipase / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification*; D008899:Mineral Oil / Q000737:chemistry / Q000378:metabolism; D059392:Petroleum Pollution; D013997:Time Factors; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000378:metabolism
chemical_list: D003470:Culture Media; D005656:Fungal Proteins; D014874:Water Pollutants, Chemical; D008899:Mineral Oil; D008049:Lipase