title: Sensitive and specific phenotypic assay for metallo-beta-lactamase detection in Enterobacteria by use of a moxalactam disk supplemented with EDTA.
abstract: Moxalactam is highly hydrolyzed by plasmid-mediated metallo-β-lactamases (MBLs), whereas it is poorly inactivated by serine-active carbapenemases. This study demonstrated that moxalactam resistance constituted an effective screen for MBL expression in enterobacteria, which could be confirmed, even in low-MBL-producing isolates, by a disk potentiation test using moxalactam and EDTA.
authors.affiliation: Service de Bactériologie, Centre Hospitalo-Universitaire d'Amiens, Hôpital Nord, 80054 Amiens, France.
authors.forename: Emilie
authors.initials: E
authors.lastname: Pluquet
authors.forename: Guillaume
authors.initials: G
authors.lastname: Arlet
authors.forename: Edouard
authors.initials: E
authors.lastname: Bingen
authors.forename: Laurence
authors.initials: L
authors.lastname: Drieux
authors.forename: Hedi
authors.initials: H
authors.lastname: Mammeri
mesh_terms: D003470:Culture Media / Q000737:chemistry*; D004492:Edetic Acid / Q000378:metabolism; D004755:Enterobacteriaceae / Q000201:enzymology*; D008826:Microbial Sensitivity Tests / Q000379:methods; D009070:Moxalactam / Q000494:pharmacology*; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000096:biosynthesis*; D047090:beta-Lactams / Q000494:pharmacology*
chemical_list: D003470:Culture Media; D047090:beta-Lactams; D004492:Edetic Acid; D001618:beta-Lactamases; D009070:Moxalactam