title: Novel genes and cellular pathways related to infection with adenovirus-36 as an obesity agent in human mesenchymal stem cells.
abstract: OBJECTIVE
  The objective of this study was to investigate the molecular mechanisms underlying adenovirus-36 (Ad-36)-induced obesity by the identification of novel genes and cellular pathways.
  
  
  METHODS
  Viral growth, intracellular lipid accumulation and gene expression profiles were determined in human mesenchymal stem cells (hMSCs) infected with Ad-36 or Ad-2. A microarray assay and gene set enrichment analysis (GSEA) were performed to assess alterations in global gene expression profiles.
  
  
  RESULTS
  Ad-36, but not Ad-2, induced lipid accumulation and upregulated adipogenesis-related genes. There was no difference in viral growth between Ad-36 infection and Ad-2 infection in hMSCs. GSEA revealed that Ad-36 infection was more frequently associated with activation of novel pathways, including the PPAR-gamma signaling pathway, and inflammation compared with Ad-2 infection, raising the possibility that these pathways may be key regulators of Ad-36-induced adipogenesis.
  
  
  CONCLUSIONS
  This study may help foster a better understanding of the roles of several cellular factors in Ad-36-induced obesity.
authors.affiliation: Department of Biotechnology, The Catholic University of Korea, Bucheon, Korea.
authors.forename: H-N
authors.initials: HN
authors.lastname: Na
authors.forename: H
authors.initials: H
authors.lastname: Kim
authors.forename: J-H
authors.initials: JH
authors.lastname: Nam
mesh_terms: D017501:Adenovirus E1B Proteins / Q000235:genetics* / Q000378:metabolism; D000260:Adenoviruses, Human / Q000235:genetics; D050156:Adipogenesis / Q000235:genetics*; D002454:Cell Differentiation; D006801:Humans; D050356:Lipid Metabolism / Q000235:genetics*; D059630:Mesenchymal Stem Cells / Q000378:metabolism / Q000821:virology*; D009765:Obesity / Q000235:genetics*; D009856:Oncogene Proteins, Viral / Q000235:genetics / Q000378:metabolism*; D047495:PPAR gamma / Q000378:metabolism; D010446:Peptide Fragments / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics
chemical_list: D017501:Adenovirus E1B Proteins; D009856:Oncogene Proteins, Viral; D047495:PPAR gamma; D010446:Peptide Fragments; C119311:adenovirus E1B protein (368-381)