title: Determination of N-acetylaspartic acid concentration in the mouse brain using HPLC with fluorescence detection.
abstract: The concentration of brain N-acetylaspartic acid (NAA) in mice was determined by high-performance liquid chromatography (HPLC) using fluorescence detection after pre-column derivatization with 4-N,N-dimethylaminosulfonyl-7-N-(2-aminoethyl)amino-2,1,3-benzoxadiazole (DBD-ED). Six different brain parts, namely, the prefrontal cortex, olfactory bulb, nucleus accumbens, striatum, cerebellum and hippocampus, of male C57BL6/J mice, were investigated. The NAA concentration (nmol/mg protein) was highest in the olfactory bulb (58.2 ± 4.0, n = 8) and lowest in the hippocampus (42.8 ± 1.6, n = 8). The proposed HPLC method with fluorescence detection was successfully used to determine the NAA concentration in each investigated brain area.
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Toho University, 2-2-1 Miyama, Funabashi-shi, Chiba 274-8510, Japan.
authors.forename: Ziyu
authors.initials: Z
authors.lastname: Song
authors.forename: Dan
authors.initials: D
authors.lastname: Ge
authors.forename: Kana
authors.initials: K
authors.lastname: Ishii
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yamada
authors.forename: Kazuya
authors.initials: K
authors.lastname: Toriumi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Watanabe
authors.forename: Toshitaka
authors.initials: T
authors.lastname: Nabeshima
authors.forename: Takeshi
authors.initials: T
authors.lastname: Fukushima
mesh_terms: D000818:Animals; D001224:Aspartic Acid / Q000031:analogs & derivatives* / Q000032:analysis; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009928:Organ Specificity; D010069:Oxadiazoles; D011506:Proteins / Q000032:analysis; D013050:Spectrometry, Fluorescence
chemical_list: D010069:Oxadiazoles; D011506:Proteins; D001224:Aspartic Acid; C000179:N-acetylaspartate