title: Growth inhibition and induction of stress protein, GroEL, of Bacillus cereus exposed to antibacterial peptide isolated from Bacillus subtilis SC-8.
abstract: This study was conducted to investigate the antibacterial effect of BSAP-254 on Bacillus cereus with the induced stress proteins. The BSAP-254 is an antimicrobial peptide isolated from soybean-fermenting bacteria, Bacillus subtilis SC-8. It had a narrow spectrum of activity against B. cereus group. The growth inhibitory effect of BSAP-254 (50 μg/mL) reduced the population of B. cereus from >10(8) to 10(4) colony-forming units per milliliter within 30 min. In B. cereus exposed to BSAP-254, 14 intracellular proteins were differentially expressed as determined by 2-DE coupled with MS. Of the differentially expressed proteins identified, the stress protein GroEL, which is heat shock protein, was induced in B. cereus exposed to antibacterial peptide.
authors.affiliation: Department of Biotechnology (BK21 Program), Chung-Ang University, An-seong, 456-756, Republic of Korea.
authors.forename: Nam Keun
authors.initials: NK
authors.lastname: Lee
authors.forename: In-Cheol
authors.initials: IC
authors.lastname: Yeo
authors.forename: Joung Whan
authors.initials: JW
authors.lastname: Park
authors.forename: Young Tae
authors.initials: YT
authors.lastname: Hahm
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D001409:Bacillus cereus / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism*; D001412:Bacillus subtilis / Q000737:chemistry*; D001426:Bacterial Proteins / Q000096:biosynthesis*; D018834:Chaperonin 60 / Q000096:biosynthesis*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D018834:Chaperonin 60