title: Black tea and theaflavins suppress various inflammatory modulators and i-NOS mediated nitric oxide synthesis during gastric ulcer healing.
abstract: The modulation of the cyclooxygenase-independent pathway by black tea (BT) and its constituent theaflavins (TFs) during their healing action against indomethacin-induced stomach ulceration in mice was investigated. On the 3(rd) day of its administration, indomethacin (18 mg/kg) induced maximum stomach ulceration, which was associated with increased myeloperoxidase (MPO) activity (93.3%, p<0.001), and inducible nitric oxide synthase (iNOS) expression (1.6-fold, p<0.001), along with augmented levels of serum nitrite (1.5-fold, p<0.001), selectins and cell adhesion molecules (CAMs), as well as reduced endothelial nitric oxide synthase (eNOS) expression (60%, p<0.001). Treatment with BT (40 mg/kg) and TF (1 mg/kg) for 3 days reversed these parameters and provided excellent (78-81%) ulcer healing. However, alterations of NOS expressions and levels of selectins and CAMs were only partially responsible for the excellent healing capacity (∼80%) of omeprazole (3 mg/kg × 3 days).
authors.affiliation: Department of Biochemistry, Dr. B.C. Roy Post Graduate Institute of Basic Medical Sciences & IPGME&R, 244B, Acharya Jagadish Chandra Bose Road, Kolkata - 700 020, India.
authors.forename: Biplab
authors.initials: B
authors.lastname: Adhikary
authors.forename: Sudhir K
authors.initials: SK
authors.lastname: Yadav
authors.forename: Saswati
authors.initials: S
authors.lastname: Chand
authors.forename: Sandip K
authors.initials: SK
authors.lastname: Bandyopadhyay
authors.forename: Subrata
authors.initials: S
authors.lastname: Chattopadhyay
mesh_terms: D000818:Animals; D044946:Biflavonoids / Q000627:therapeutic use*; D002392:Catechin / Q000627:therapeutic use*; D015815:Cell Adhesion Molecules / Q000097:blood; D015151:Immunoblotting; D007213:Indomethacin; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis*; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism*; D052250:Nitric Oxide Synthase Type III / Q000096:biosynthesis / Q000097:blood / Q000235:genetics; D009573:Nitrites / Q000097:blood; D009853:Omeprazole / Q000494:pharmacology; D009195:Peroxidase / Q000378:metabolism; D019087:Selectins / Q000097:blood; D013276:Stomach Ulcer / Q000139:chemically induced / Q000188:drug therapy*; D013662:Tea / Q000737:chemistry*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D044946:Biflavonoids; D015815:Cell Adhesion Molecules; D009573:Nitrites; D019087:Selectins; D013662:Tea; C056068:theaflavin; D009569:Nitric Oxide; D002392:Catechin; D009195:Peroxidase; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III; D009853:Omeprazole; D007213:Indomethacin