title: Glycyrrhiza glabra (Linn.) and Lavandula officinalis (L.) cell suspension cultures-based biotransformation of β-artemether.
abstract: The biotransformation of β-artemether (1) by cell suspension cultures of Glycyrrhiza glabra and Lavandula officinalis is reported here for the first time. The major biotransformed product appeared as a grayish-blue color spot on thin-layer chromatography (TLC) with transparent crystal-like texture. Based on its infrared (IR) and (1)H nuclear magnetic resonance (NMR) spectra, the product was characterized as a tetrahydrofuran (THF)-acetate derivative (2). The highest conversion efficiencies of 57 and 60% were obtained when 8-9-day-old cell suspensions of G. glabra and L. officinalis were respectively fed with 4-7 mg of compound 1 in 40 ml of medium per culture and the cells were harvested after 2-5 days of incubation. The addition of compound 1 at the beginning of the culture cycle caused severe growth depression in a dose-dependent manner, resulting in poor bioconversion efficiency of ~25% at 2-5 mg/culture dose only.
authors.affiliation: Central Institute of Medicinal and Aromatic Plants, Lucknow, 226015, India.
authors.forename: Suman
authors.initials: S
authors.lastname: Patel
authors.forename: Rashmi
authors.initials: R
authors.lastname: Gaur
authors.forename: Mohita
authors.initials: M
authors.lastname: Upadhyaya
authors.forename: Archana
authors.initials: A
authors.lastname: Mathur
authors.forename: Ajay K
authors.initials: AK
authors.lastname: Mathur
authors.forename: Rajendra S
authors.initials: RS
authors.lastname: Bhakuni
mesh_terms: D000077549:Artemether; D037621:Artemisinins / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D002478:Cells, Cultured; D002855:Chromatography, Thin Layer; D006035:Glycyrrhiza / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D027523:Lavandula / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure
chemical_list: D037621:Artemisinins; D000077549:Artemether