title: Cutting edge: Identification of a motile IL-17-producing gammadelta T cell population in the dermis.
abstract: Dendritic epidermal T cells (DETCs) are a well-studied population of γδ T cells that play important roles in wound repair. In this study, we characterize a second major population of γδ T cells in the skin that is present in the dermis. In contrast to DETCs, these Vγ5-negative cells are IL-7R(hi)CCR6(hi) retinoic acid-related orphan receptor γt(+) and are precommitted to IL-17 production. Dermal γδ T cells fail to reconstitute following irradiation and bone marrow transplantation unless the mice also receive a transfer of neonatal thymocytes. Real-time intravital imaging of CXCR6(GFP/+) mouse skin reveals dermal γδ T cells migrate at ∼4 μm/min, whereas DETCs are immobile. Like their counterparts in peripheral lymph nodes, dermal γδ T cells rapidly produce IL-17 following exposure to IL-1β plus IL-23. We have characterized a major population of skin γδ T cells and propose that these cells are a key source of IL-17 in the early hours after skin infection.
authors.affiliation: Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA 94143, USA.
authors.forename: Elizabeth E
authors.initials: EE
authors.lastname: Gray
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Suzuki
authors.forename: Jason G
authors.initials: JG
authors.lastname: Cyster
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002465:Cell Movement / Q000276:immunology; D020405:Dermis / Q000276:immunology* / Q000378:metabolism; D005434:Flow Cytometry; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D020381:Interleukin-17 / Q000276:immunology* / Q000378:metabolism; D053583:Interleukin-1beta / Q000494:pharmacology; D053759:Interleukin-23 / Q000494:pharmacology; D008198:Lymph Nodes / Q000276:immunology / Q000378:metabolism; D051379:Mice; D057507:Mice, 129 Strain; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D054399:Receptors, CCR6 / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D012867:Skin / Q000276:immunology / Q000378:metabolism; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D013997:Time Factors
chemical_list: C498126:CCR6 protein, mouse; D020381:Interleukin-17; D053583:Interleukin-1beta; D053759:Interleukin-23; D016692:Receptors, Antigen, T-Cell, gamma-delta; D054399:Receptors, CCR6; D049452:Green Fluorescent Proteins