title: Systemic and mucosal immunity induced by attenuated Salmonella enterica serovar Typhimurium expressing ORF7 of porcine reproductive and respiratory syndrome virus.
abstract: Oral administration of attenuated Salmonella vaccine may provide valuable advantages such as low cost, easy preparation, and safety. Attenuated Salmonella vaccines also serve as carriers of foreign antigens and immunomodulatory cytokines. Presently, an attenuated Salmonella enterica serovar Typhimurium strain was used as a carrier for open reading frame 7 (ORF7) protein of porcine reproductive and respiratory syndrome virus (PRRSV), a swine pathogen of significant global economic importance. Initially, an attenuated S. enterica serovar Typhimurium expressing ORF7 gene derived from PRRSV Korean isolate was constructed. Following oral administration of a single dose of the attenuated Salmonella vaccine expressing PRRSV ORF7, humoral and cell-mediated immune responses specific for ORF7 were induced at both systemic and mucosal sites including spleen, mesenteric lymph node, Peyer's patch, and laminar propria, as evaluated by determining serum ORF7-specific IgG and mucosal IgA responses, as well as Th1- and Th2-type cytokine production from antigen-stimulated T cells. The induced humoral responses were sustained for at least 12weeks post-immunization. In particular, the immunized mice displayed immune responses to both the foreign ORF7 antigen and Salmonella itself. The results indicate the value of attenuated S. enterica serovar Typhimurium as an oral carrier of PRRSV antigenic proteins to induce effective systemic and mucosal immunity.
authors.affiliation: College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University, Jeonju, Republic of Korea.
authors.forename: Young Woo
authors.initials: YW
authors.lastname: Han
authors.forename: Seong Bum
authors.initials: SB
authors.lastname: Kim
authors.forename: Masudur
authors.initials: M
authors.lastname: Rahman
authors.forename: Erdenebileg
authors.initials: E
authors.lastname: Uyangaa
authors.forename: Byung Min
authors.initials: BM
authors.lastname: Lee
authors.forename: Jin Hyoung
authors.initials: JH
authors.lastname: Kim
authors.forename: Ki In
authors.initials: KI
authors.lastname: Park
authors.forename: Jin Tae
authors.initials: JT
authors.lastname: Hong
authors.forename: Sang-Bae
authors.initials: SB
authors.lastname: Han
authors.forename: Seong Kug
authors.initials: SK
authors.lastname: Eo
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000942:Antigens, Bacterial; D000956:Antigens, Viral / Q000276:immunology; D001483:Base Sequence; D002478:Cells, Cultured; D005260:Female; D018928:Immunity, Mucosal; D007070:Immunoglobulin A / Q000032:analysis / Q000096:biosynthesis; D007074:Immunoglobulin G / Q000032:analysis / Q000096:biosynthesis; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology* / Q000382:microbiology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology* / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics / Q000276:immunology; D010581:Peyer's Patches / Q000166:cytology / Q000276:immunology* / Q000382:microbiology; D019316:Porcine respiratory and reproductive syndrome virus / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D012481:Salmonella Infections, Animal / Q000276:immunology / Q000378:metabolism / Q000517:prevention & control*; D012486:Salmonella typhimurium / Q000254:growth & development / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology* / Q000382:microbiology; D013552:Swine; D058408:Th1-Th2 Balance; D014611:Vaccination; D014613:Vaccines, Attenuated / Q000008:administration & dosage*
chemical_list: D000942:Antigens, Bacterial; D000956:Antigens, Viral; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D014613:Vaccines, Attenuated