title: Development and characterization of a Chinese hamster ovary cell-specific oligonucleotide microarray.
abstract: The Chinese hamster ovary (CHO) cell line is one of the most widely used mammalian cell lines for biopharmaceutical production. We have developed and characterized a gene expression microarray (WyeHamster2a) specific for CHO cells that has enabled the study of ~3,500 sequences. Analysis of multiple sets of replicate scans showed that data derived from the WyeHamster2a array is highly reproducible confirming it as a robust tool for profiling. Twelve gene sequences were selected for follow-up RT-qPCR to confirm the accuracy and precision of the microarray results. In all but the most subtle gene expression differences, the microarray proved to be a reliable measure of differential gene expression. Finally, we were able to quantify the difference between using a bona fide CHO-specific microarray for profiling CHO cells versus an alternate, commercially available, rodent microarray such as a mouse or rat-specific format.
authors.affiliation: Pfizer, 1 Burtt Rd, Andover, MA 01810, USA. Mark.Melville@pfizer.com
authors.forename: Mark
authors.initials: M
authors.lastname: Melville
authors.forename: Padraig
authors.initials: P
authors.lastname: Doolan
authors.forename: William
authors.initials: W
authors.lastname: Mounts
authors.forename: Niall
authors.initials: N
authors.lastname: Barron
authors.forename: Louane
authors.initials: L
authors.lastname: Hann
authors.forename: Mark
authors.initials: M
authors.lastname: Leonard
authors.forename: Martin
authors.initials: M
authors.lastname: Clynes
authors.forename: Tim
authors.initials: T
authors.lastname: Charlebois
mesh_terms: D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction