title: Epidemiological survey of Crimean-Congo hemorrhagic fever virus in Yunnan, China, 2008.
abstract: OBJECTIVE
  We aimed to determine the seroprevalence of Crimean-Congo hemorrhagic fever virus (CCHFV) infection in Yunnan Province, China.
  
  
  METHODS
  One thousand six hundred and fifty-seven human serum samples and 1280 ticks (Hyalomma asiaticum) were collected from five counties (Menglian, Menghai, Lancang, Mengla, and Ximeng). Serum samples were analyzed independently by indirect immunofluorescence assay and Western blotting to detected CCHFV antibody. The ticks were examined by reverse transcriptase-polymerase chain reaction (RT-PCR) to detect virus RNA.
  
  
  RESULTS
  The CCHFV IgG positivity was 3.4% (57/1657). A multivariate analysis was performed, and variables that increased the chance of infection were found to include history of tick bite or contact (odds ratio (OR) 16.6, 95% confidence interval (CI) 7.5-37.0) and age>30 years (OR 6.8, 95% CI 1.6-28.2). The RT-PCR positive rate for ticks was 14.3% (6/42).
  
  
  CONCLUSIONS
  The five counties (Menglian, Menghai, Lancang, Mengla, and Ximeng) in Yunnan are areas with the potential for CCHF outbreaks. Residents should protect themselves against tick bites and the surveillance of CCHFV in this region should be improved.
authors.affiliation: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.
authors.forename: Han
authors.initials: H
authors.lastname: Xia
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.forename: Jialin
authors.initials: J
authors.lastname: Yang
authors.forename: Limin
authors.initials: L
authors.lastname: Pan
authors.forename: Jiuru
authors.initials: J
authors.lastname: Zhao
authors.forename: Zhengyou
authors.initials: Z
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Hongpin
authors.initials: H
authors.lastname: Zhou
authors.forename: Ying
authors.initials: Y
authors.lastname: Dong
authors.forename: Shan
authors.initials: S
authors.lastname: Guo
authors.forename: Shuang
authors.initials: S
authors.lastname: Tang
authors.forename: Zhong
authors.initials: Z
authors.lastname: Zhang
authors.forename: Zhaojun
authors.initials: Z
authors.lastname: Fan
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Hu
authors.forename: Zheng
authors.initials: Z
authors.lastname: Kou
authors.forename: Tianxian
authors.initials: T
authors.lastname: Li
mesh_terms: D000328:Adult; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002681:China / Q000453:epidemiology; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D003225:Hemorrhagic Fever Virus, Crimean-Congo / Q000145:classification / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification*; D006479:Hemorrhagic Fever, Crimean / Q000175:diagnosis / Q000453:epidemiology* / Q000821:virology; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D026863:Ixodidae / Q000821:virology*; D008297:Male; D008875:Middle Aged; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012307:Risk Factors; D016036:Seroepidemiologic Studies; D055815:Young Adult
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D012367:RNA, Viral