title: FvbA is required for vibriobactin utilization in Pseudomonas aeruginosa.
abstract: Bacteria acquire iron through a highly specific mechanism involving iron-chelating molecules termed siderophores. The Gram-negative bacterium Pseudomonas aeruginosa can utilize siderophores produced by other micro-organisms to facilitate iron uptake. Here we show that a P. aeruginosa strain deficient in siderophore production can use the Vibrio cholerae siderophore vibriobactin as an iron source. In addition, we identified a P. aeruginosa gene, PA4156 (fvbA), encoding a protein highly homologous to the V. cholerae vibriobactin receptor (ViuA). A P. aeruginosa mutant in the two endogenous siderophores (pyoverdine and pyochelin) and in fvbA was unable to utilize vibriobactin as an iron source. Additionally, preliminary analyses revealed the involvement of vibriobactin, Fur protein and an IclR-type regulator, FvbR (PA4157), in fvbA regulation.
authors.affiliation: The Bacterial Biofilm Research Laboratory, The Institute for Nanotechnology and Advanced Materials, The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat Gan 52900, Israel.
authors.forename: Sivan
authors.initials: S
authors.lastname: Elias
authors.affiliation: The Bacterial Biofilm Research Laboratory, The Institute for Nanotechnology and Advanced Materials, The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat Gan 52900, Israel.
authors.forename: Elena
authors.initials: E
authors.lastname: Degtyar
authors.affiliation: The Bacterial Biofilm Research Laboratory, The Institute for Nanotechnology and Advanced Materials, The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat Gan 52900, Israel.
authors.forename: Ehud
authors.initials: E
authors.lastname: Banin
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D002396:Catechols / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D007501:Iron / Q000378:metabolism*; D009842:Oligopeptides / Q000235:genetics; D010080:Oxazoles / Q000378:metabolism*; D010636:Phenols; D016133:Polymerase Chain Reaction; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000378:metabolism*; D011956:Receptors, Cell Surface; D012097:Repressor Proteins / Q000378:metabolism; D017262:Siderophores / Q000235:genetics / Q000378:metabolism*; D013844:Thiazoles; D014734:Vibrio cholerae / Q000201:enzymology
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002396:Catechols; D009842:Oligopeptides; D010080:Oxazoles; D010636:Phenols; D011956:Receptors, Cell Surface; D012097:Repressor Proteins; D017262:Siderophores; D013844:Thiazoles; C068477:ferric uptake regulating proteins, bacterial; C025316:pyochelin; C042453:pyoverdin; C040227:vibriobactin; D007501:Iron