title: Small interfering RNAs screened from random siRNA library direct neuronal differentiation.
abstract: Directed neuronal differentiation is crucial for development of cell therapy and investigation of neurogenesis. However, limited differentiation-inducing agents are available and most of current differentiation regimens are complicated. Here we carried out a combinatorial high-throughput screen with a random siRNA library on murine P19 cell differentiation toward neuronal lineage. Two siRNAs screened from the library were able to direct neuronal differentiation, determined by nestin, neurofilament-M and MAP-2 up-regulation. This is the first trial of a screening procedure for neuronal differentiation-directing agents arising from a random siRNA library and demonstrates that a random siRNA library can be considered as a new resource in efforts to seek new agents for directed differentiation. As the random siRNA library has a broad coverage for the entire genome, screening using random siRNA library can be expected to greatly augment the repertoire of siRNAs for directed differentiation.
authors.affiliation: National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (CAMS)/School of Basic Medicine, Peking Union Medical College (PUMC), No. 5, Dong-Dan-San-Tiao, Beijing 100005, People's Republic of China.
authors.forename: Yabin
authors.initials: Y
authors.lastname: Lu
authors.forename: Jiewen
authors.initials: J
authors.lastname: Zhang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Xiong
authors.forename: Ning
authors.initials: N
authors.lastname: Zhu
authors.forename: Cuiqing
authors.initials: C
authors.lastname: Fan
authors.forename: Song
authors.initials: S
authors.lastname: Wang
authors.forename: Shaowei
authors.initials: S
authors.lastname: Liu
authors.forename: Zicai
authors.initials: Z
authors.lastname: Liang
authors.forename: Yan
authors.initials: Y
authors.lastname: Shen
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wang
authors.forename: Meihong
authors.initials: M
authors.lastname: Chen
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D045744:Cell Line, Tumor; D020868:Gene Silencing; D057166:High-Throughput Screening Assays / Q000379:methods*; D051379:Mice; D009474:Neurons / Q000187:drug effects*; D034741:RNA, Small Interfering / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D034741:RNA, Small Interfering