title: Detecting DNA methylation through changes in transverse proton relaxation.
abstract: We present a facile, simple method to detect DNA methylation by measuring the transverse proton relaxation behaviour. Positively charged nanoparticles are arranged along the negatively charged backbone of DNA strands through electrostatic interactions. The arrangement of NPs along DNA strands aids to amplify and compare the transverse proton relaxation signal for un-cut versus cut DNA strands cleaved by sequence specific restriction enzymes. Results from this study suggest that the presence of methylation on DNA can be detected using superparamagnetic NPs using NMR.
authors.affiliation: Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN, USA.
authors.forename: Hamsa
authors.initials: H
authors.lastname: Jaganathan
authors.forename: Pengfei
authors.initials: P
authors.lastname: Wang
authors.forename: Jonathon
authors.initials: J
authors.lastname: Klein
authors.forename: Amy C
authors.initials: AC
authors.lastname: Lossie
authors.forename: Albena
authors.initials: A
authors.lastname: Ivanisevic
mesh_terms: D004247:DNA / Q000032:analysis*; D019175:DNA Methylation; D015257:DNA-Cytosine Methylases / Q000378:metabolism; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry; D011522:Protons; D055672:Static Electricity
chemical_list: D011522:Protons; D004247:DNA; C029641:DNA modification methylase HpaII; D015257:DNA-Cytosine Methylases