title: Lectin histochemistry of respiratory mucosa in the Pacific white-sided dolphin.
abstract: Sugars in the glycocalyx play an important role in the attachment of infectious agents to the respiratory mucosa. We examined the histochemistry of 23 lectins to survey the sugar expression in the glycocalyx of the respiratory mucosa of the Pacific white-sided dolphin, Lagenorhynchus obliquidens. The ciliated and basal cells were positive for all of the lectins studied. SBA, WFA, GSL-II, STL, S-WGA, and PNA staining in the cytoplasm showed different intensities between basal cells and ciliated cells. These results suggest that multiple terminal glycosylation occurs on ciliated and basal cells, such as GalNAc, GlcNAc, NeuNAc, galactose, glucose/mannose, oligosaccharide, and fucose, and that sugar residue expression changes during cell differentiation. The Pacific white-sided dolphin respiratory mucosa might express multiple sugar residues in the glycocalyx, to prevent the attachment and colonisation of infectious agents.
authors.affiliation: Department of Anatomy and Embryology, Ehime University Graduate School of Medicine, To-on, Ehime 791–0295, Japan. tshimo@m.ehime-u.ac.jp
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Shimokawa
authors.forename: Takuya
authors.initials: T
authors.lastname: Doihara
authors.forename: Manami
authors.initials: M
authors.lastname: Makara
authors.forename: Kyojy
authors.initials: K
authors.lastname: Miyawaki
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Nabeka
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Wakisaka
authors.forename: Naoto
authors.initials: N
authors.lastname: Kobayashi
authors.forename: Seiji
authors.initials: S
authors.lastname: Matsuda
mesh_terms: D000818:Animals; D004289:Dolphins; D005786:Gene Expression Regulation / Q000502:physiology; D037102:Lectins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008297:Male; D020545:Respiratory Mucosa / Q000737:chemistry / Q000378:metabolism*
chemical_list: D037102:Lectins