title: Electrophoretic detection of genetic variability among Schistosoma japonicum isolates by sequence-related amplified polymorphism.
abstract: In the present study, sequence-related amplification polymorphism (SRAP) was utilized to study the genetic variability among Schistosoma japonicum isolates from different provinces in China, using Schistosoma mansoni from Puerto Rico for comparison. Five out of ten tested SRAP primer combinations displayed significant polymorphisms among S. japonicum isolates from China, namely ME2/EM1, ME4/EM1, ME4/EM6, ME5/EM4 and ME5/EM5. Analysis of the 61 S. japonicum samples from China with five SRAP primer combinations identified a total of 83 reproducible polymorphic fragments. The number of fragments using each primer combination ranged from 14 to 19, with an average of 16 polymorphic bands per primer pair, and the size of fragment ranged approximately from 100 to 1000 bp. Representative-specific SRAP fragments were excised from the gels, and confirmed by PCR amplification of genomic DNA using primers designed and based on the sequences of these SRAP fragments. Based on SRAP profiles, unweighted pair-group method with arithmetic averages (UPGMA) dendrogram was constructed. UPGMA clustering algorithm categorized S. japonicum isolates from China into nine clades and two lineages (representing the mountainous and lake/marshland regions). These results indicate the usefulness of the SRAP technique for revealing genetic variability among S. japonicum isolates from China, and the SRAP technique should be applicable to other living organisms.
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, CAAS, Lanzhou, Gansu Province, P. R. China.
authors.forename: Hui-Qun
authors.initials: HQ
authors.lastname: Song
authors.forename: Xi-Hao
authors.initials: XH
authors.lastname: Mo
authors.forename: Guang-Hui
authors.initials: GH
authors.lastname: Zhao
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.forename: Feng-Cai
authors.initials: FC
authors.lastname: Zou
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Xiang-Yun
authors.initials: XY
authors.lastname: Wu
authors.forename: Rui-Qing
authors.initials: RQ
authors.lastname: Lin
authors.forename: Ya-Biao
authors.initials: YB
authors.lastname: Weng
authors.forename: Xing-Quan
authors.initials: XQ
authors.lastname: Zhu
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D004247:DNA / Q000032:analysis / Q000235:genetics* / Q000302:isolation & purification; D017931:DNA Primers; D004587:Electrophoresis, Agar Gel; D005260:Female; D006801:Humans; D008297:Male; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D015203:Reproducibility of Results; D012549:Schistosoma japonicum / Q000235:genetics* / Q000302:isolation & purification; D012550:Schistosoma mansoni / Q000235:genetics; D012554:Schistosomiasis japonica / Q000469:parasitology
chemical_list: D017931:DNA Primers; D004247:DNA