title: Using a starch-rich mutant of Arabidopsis thaliana as feedstock for fermentative hydrogen production.
abstract: A mutant plant (Arabidopsis thaliana), sex1-1 (starch excess 1-1), accumulating high starch content in leaves was created to serve as better biomass feedstock for a H2-producing strain Clostridium butyricum CGS2, which efficiently utilizes starch for H2 production but cannot assimilate cellulosic materials. The starch content of the mutant plant increased to 10.67 mg/fresh weight, which is four times higher than that of wild type plant. Using sex1-1 mutant plant as feedstock, C. butyricum CGS2 could produce 490.4 ml/l of H2 with a H2 production rate of 32.9 ml/h/l. The H2 production performance appeared to increase with the increase in the concentration of mutant plant from 2.5 to 10 g/l. The highest H2 to plant biomass yield was nearly 49 ml/g for the mutant plant. This study successfully demonstrated the feasibility of using a starch-rich mutant plant for more effective bioH2 production with C. butyricum CGS2.
authors.affiliation: Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Yung-Chung
authors.initials: YC
authors.lastname: Lo
authors.forename: Li-Fen
authors.initials: LF
authors.lastname: Huang
authors.forename: Chieh-Lun
authors.initials: CL
authors.lastname: Cheng
authors.forename: Jychian
authors.initials: J
authors.lastname: Chen
authors.forename: Jo-Shu
authors.initials: JS
authors.lastname: Chang
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics; D056804:Biofuels / Q000032:analysis; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D002244:Carbon / Q000494:pharmacology; D002482:Cellulose / Q000378:metabolism; D003352:Corynebacterium / Q000187:drug effects / Q000302:isolation & purification; D005285:Fermentation / Q000187:drug effects / Q000502:physiology*; D006859:Hydrogen / Q000378:metabolism*; D009004:Monosaccharide Transport Proteins / Q000235:genetics; D009154:Mutation / Q000235:genetics*; D012995:Solubility / Q000187:drug effects; D013213:Starch / Q000096:biosynthesis / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D056804:Biofuels; D002244:Carbon; D002482:Cellulose; D006859:Hydrogen; D009004:Monosaccharide Transport Proteins; D013213:Starch; C435922:At1g10760 protein, Arabidopsis