title: Linalool from rosewood (Aniba rosaeodora Ducke) oil inhibits adenylate cyclase in the retina, contributing to understanding its biological activity.
abstract: Rosewood oil (RO) (Aniba rosaeodora Ducke) is rich in linalool, a monoterpene alcohol, which has well studied anxiolytic, sedative and anticonvulsant effects. The inhibition of the increases in cAMP protects against seizures in a diversity of models of epilepsy. In this paper, the principal aim was to investigate the effects of RO, (±)-linalool and (-)-linalool) on adenylate cyclase. They were tested in chick retinas and forskolin was used to stimulate the enzyme target. The phosphodiesterase inhibitor, 4-(3-butoxy-4-methoxybenzyl)-imidazolidin-2-one, and the non-selective adenosine receptor antagonist 3-isobutyl-methyl-xanthine (IBMX), were used to control the participation of phosphodiesterase and adenosine receptors in the resulting effects, respectively. The cAMP accumulation was measured by enzyme immune assay (EIA). Rosewood oil, (-)-linalool and (±)-linalool inhibited exclusively the cAMP accumulation stimulated by forskolin, even when adenosine receptors were blocked with IBMX. The IC(50) values (in μ m concentration range) calculated from their concentration response-curves were not statistically different, however, the compounds presented a different relative efficacy. These results extend the range of subcellular mechanisms underlying the relaxant action of linalool on the central nervous system.
authors.affiliation: Laboratório de Bioquímica do Desenvolvimento do Sistema Nervoso, Instituto de Ciências Biológicas, Universidade Federal do Pará, 66075-110 Belém, PA, Brazil. lsampaio@ufpa.br
authors.forename: Lucia de Fatima S
authors.initials: Lde F
authors.lastname: Sampaio
authors.forename: José Guilherme S
authors.initials: JG
authors.lastname: Maia
authors.forename: Amanda M
authors.initials: AM
authors.lastname: de Parijós
authors.forename: Rita Z
authors.initials: RZ
authors.lastname: de Souza
authors.forename: Lauro Euclides S
authors.initials: LE
authors.lastname: Barata
mesh_terms: D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000080462:Acyclic Monoterpenes; D000067956:Adenylyl Cyclase Inhibitors; D000818:Animals; D002491:Central Nervous System Agents / Q000494:pharmacology*; D002645:Chickens; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D020128:Inhibitory Concentration 50; D019770:Lauraceae / Q000737:chemistry*; D039821:Monoterpenes / Q000494:pharmacology*; D009822:Oils, Volatile / Q000737:chemistry / Q000494:pharmacology*; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D018047:Receptors, Purinergic P1 / Q000378:metabolism; D012160:Retina / Q000187:drug effects* / Q000201:enzymology
chemical_list: D000080462:Acyclic Monoterpenes; D000067956:Adenylyl Cyclase Inhibitors; D002491:Central Nervous System Agents; D004791:Enzyme Inhibitors; D039821:Monoterpenes; D009822:Oils, Volatile; D010936:Plant Extracts; D018047:Receptors, Purinergic P1; D005576:Colforsin; C018584:linalool; D000242:Cyclic AMP; D010727:Phosphoric Diester Hydrolases; D015056:1-Methyl-3-isobutylxanthine