title: Aminopropyl-functionalized cubic Ia3d mesoporous silica nanoparticle as an efficient support for immobilization of superoxide dismutase.
abstract: In this research, the immobilization of superoxide dismutase (SOD) onto aminopropyl-functionalized KIT-6 [n-PrNH(2)-KIT-6] was investigated. This organo-functionalized mesoporous silica nanoparticle was prepared using a non-ionic surfactant and was fully characterized by XRD, nitrogen adsorption-desorption isotherm assay, IR and TGA techniques. An activity assay demonstrated that the immobilized SOD had a higher activity than the free enzyme. Further investigations using FT-IR, circular dichroism (CD), and probe 1-anilino-8-naphthalene sulfonate (ANS) fluorescence intensity measurements indicated that the structure of the enzyme did not change upon binding to the mesoporous silica, and that immobilized SOD was also less affected by higher temperatures. The melting temperatures of the free and immobilized enzymes were measured by differential scanning calorimetry (DSC), which showed that a fraction of immobilized enzyme was more stable and revealed that immobilized enzyme was partly reversible.
authors.affiliation: Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Falahati
authors.forename: Leila
authors.initials: L
authors.lastname: Ma'mani
authors.forename: Ali Akbar
authors.initials: AA
authors.lastname: Saboury
authors.forename: Abbas
authors.initials: A
authors.lastname: Shafiee
authors.forename: Alireza
authors.initials: A
authors.lastname: Foroumadi
authors.forename: Ali Reza
authors.initials: AR
authors.lastname: Badiei
mesh_terms: D000327:Adsorption; D002152:Calorimetry, Differential Scanning; D002942:Circular Dichroism; D004800:Enzymes, Immobilized / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry; D013482:Superoxide Dismutase / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D004800:Enzymes, Immobilized; D012822:Silicon Dioxide; D013482:Superoxide Dismutase