title: Oxidative stress and hypoxia-induced factor 1α expression in gastric ischemia.
abstract: OBJECTIVE
  To investigate the relation of reactive oxygen species (ROS) to hypoxia induced factor 1α (HIF-1α) in gastric ischemia.
  
  
  METHODS
  The animal model of gastric ischemia reperfusion was established by placing an elastic rubber band on the proximal part of the bilateral lower limb for ligature for 3 h and reperfusion for 0, 1, 3, 6, 12 or 24 h. Ischemic post-conditioning, three cycles of 30-s reperfusion and 30-s femoral aortic reocclusion were conducted before reperfusion. Histological and immunohistochemical methods were used to assess the gastric oxidative damage and the expression of HIF1-α in gastric ischemia. The malondialdehyde (MDA) content and superoxide dismutase (SOD), xanthine oxidase (XOD) and myeloperoxidase (MPO) activities were determined by colorimetric assays.
  
  
  RESULTS
  Ischemic post-conditioning can reduce post-ischemic oxidative stress and the expression of HIF-1α of gastric tissue resulting from limb ischemia reperfusion injury. MDA, SOD, XOD and MPO were regarded as indexes for mucosal injuries from ROS, and ROS was found to affect the expression of HIF-1α under gastric ischemic conditions.
  
  
  CONCLUSIONS
  ROS affects HIF-1α expression under gastric ischemic conditions induced by limb ischemia reperfusion injury. Therefore, ROS can regulate HIF-1α expression in gastric ischemia.
authors.affiliation: Department of Anesthesiology, The First Hospital of Lanzhou University, Lanzhou 73000, Gansu Province, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Yu-Fang
authors.initials: YF
authors.lastname: Leng
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xing
authors.initials: X
authors.lastname: Xue
authors.forename: Yu-Qing
authors.initials: YQ
authors.lastname: Kang
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D005753:Gastric Mucosa / Q000378:metabolism*; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism*; D007511:Ischemia / Q000378:metabolism*; D057775:Ischemic Postconditioning; D015227:Lipid Peroxidation; D008297:Male; D018384:Oxidative Stress; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015427:Reperfusion Injury / Q000378:metabolism; D013270:Stomach / Q000098:blood supply* / Q000473:pathology
chemical_list: D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D017382:Reactive Oxygen Species