title: Measurements of intracellular calcium signals in polarized primary cultures of normal and cystic fibrosis human airway epithelia.
abstract: The airways are continuously challenged by a variety of stimuli including bacteria, viruses, allergens, and inflammatory factors that act as agonists for G protein-coupled receptors (GPCR). Intracellular calcium (Ca(2+) (i)) mobilization in airway epithelia in response to extracellular stimuli regulates key airway innate defense functions, e.g., Ca(2+)-activated Cl(-) secretion, ciliary beating, mucin secretion, and inflammatory responses. Because Ca(2+) (i) mobilization in response to luminal stimuli is larger in CF vs. normal human airway epithelia, alterations in Ca(2+) (i) signals have been associated with the pathogenesis of CF airway disease. Hence, assessment of Ca(2+) (i) signaling has become an important area of CF research. This chapter will focus on measurements of cytoplasmic and mitochondrial Ca(2+) signals resulting from GPCR activation in polarized primary cultures of normal and CF human bronchial epithelia (HBE).
authors.affiliation: Department of Medicine, Cystic Fibrosis/Pulmonary Research and Treatment Center, The University of North Carolina, Chapel Hill, NC 27599-7248, USA. carla_ribeiro@med.unc.edu
authors.forename: Carla M P
authors.initials: CM
authors.lastname: Ribeiro
mesh_terms: D001980:Bronchi / Q000166:cytology / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D018929:Cell Culture Techniques; D016764:Cell Polarity; D002478:Cells, Cultured; D003550:Cystic Fibrosis / Q000378:metabolism* / Q000503:physiopathology; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism; D005456:Fluorescent Dyes / Q000032:analysis; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism*; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism*; D020545:Respiratory Mucosa / Q000378:metabolism* / Q000473:pathology; D015398:Signal Transduction
chemical_list: D005456:Fluorescent Dyes; D043562:Receptors, G-Protein-Coupled; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D002118:Calcium