title: Isolation of mouse pancreatic alpha, beta, duct and acinar populations with cell surface markers.
abstract: Tools permitting the isolation of live pancreatic cell subsets for culture and/or molecular analysis are limited. To address this, we developed a collection of monoclonal antibodies with selective surface labeling of endocrine and exocrine pancreatic cell types. Cell type labeling specificity and cell surface reactivity were validated on mouse pancreatic sections and by gene expression analysis of cells isolated using FACS. Five antibodies which marked populations of particular interest were used to isolate and study viable populations of purified pancreatic ducts, acinar cells, and subsets of acinar cells from whole pancreatic tissue or of alpha or beta cells from isolated mouse islets. Gene expression analysis showed the presence of known endocrine markers in alpha and beta cell populations and revealed that TTR and DPPIV are primarily expressed in alpha cells whereas DGKB and GPM6A have a beta cell specific expression profile.
authors.affiliation: Oregon Health and Science University and Oregon Stem Cell Center, 3181 SW Sam Jackson Park Rd., Portland, OR 97239, USA. dorrellc@ohsu.edu
authors.forename: Craig
authors.initials: C
authors.lastname: Dorrell
authors.forename: Maria T
authors.initials: MT
authors.lastname: Grompe
authors.forename: Fong Cheng
authors.initials: FC
authors.lastname: Pan
authors.forename: Yongping
authors.initials: Y
authors.lastname: Zhong
authors.forename: Pamela S
authors.initials: PS
authors.lastname: Canaday
authors.forename: Leonard D
authors.initials: LD
authors.lastname: Shultz
authors.forename: Dale L
authors.initials: DL
authors.lastname: Greiner
authors.forename: Chris V
authors.initials: CV
authors.lastname: Wright
authors.forename: Philip R
authors.initials: PR
authors.lastname: Streeter
authors.forename: Markus
authors.initials: M
authors.lastname: Grompe
mesh_terms: D000818:Animals; D058846:Antibodies, Monoclonal, Murine-Derived; D000954:Antigens, Surface / Q000276:immunology / Q000378:metabolism*; D002469:Cell Separation / Q000379:methods*; D019852:Diacylglycerol Kinase / Q000378:metabolism; D018819:Dipeptidyl Peptidase 4 / Q000378:metabolism; D005434:Flow Cytometry / Q000379:methods; D050416:Glucagon-Secreting Cells / Q000378:metabolism*; D050417:Insulin-Secreting Cells / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009419:Nerve Tissue Proteins / Q000378:metabolism; D010179:Pancreas / Q000166:cytology / Q000196:embryology; D010183:Pancreatic Ducts / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D011228:Prealbumin / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D013194:Staining and Labeling
chemical_list: D058846:Antibodies, Monoclonal, Murine-Derived; D000954:Antigens, Surface; C083480:Gpm6a protein, mouse; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D011228:Prealbumin; D019852:Diacylglycerol Kinase; D018819:Dipeptidyl Peptidase 4; C545245:Dpp4 protein, mouse