title: Mutational analysis of the tumor-suppressor gene wt1 - detection of a novel homozygous point mutation in sporadic unilateral wilms-tumor.
abstract: The WT1 gene located on chromosome 11p13, has been identified as the first Wilms' tumor suppressor gene and has been implicated in the development of Wilms' tumor. About 10% of Wilms' tumors analyzed to date carry a mutation and only 6 different point mutations affecting the zinc finger region have been reported. We analyzed the zinc finger coding exons of 38 sporadic Wilms' tumor by SSCP and detected 2 point mutations. One homo/hemizygous mutation, already described in the literature, replaced an arginine in zinc finger II by a stop codon. The other mutation, a replacement of an arginine by a stop codon in zinc finger I, represents a novel mutation.
authors.affiliation: UNIV ZURICH HOSP,INST CLIN PATHOL,ZURICH,SWITZERLAND. UNIV ZURICH HOSP,DEPT DERMATOL,ZURICH,SWITZERLAND. UNIV ZURICH HOSP,DEPT PATHOL,DIV CANC RES,ZURICH,SWITZERLAND.
authors.forename: R
authors.initials: R
authors.lastname: Kosters
authors.forename: V
authors.initials: V
authors.lastname: Adams
authors.forename: S
authors.initials: S
authors.lastname: Hassam
authors.forename: R
authors.initials: R
authors.lastname: Schafer
authors.forename: M
authors.initials: M
authors.lastname: Schmid
authors.forename: R
authors.initials: R
authors.lastname: Moos
authors.forename: J
authors.initials: J
authors.lastname: Briner