title: An inducible transgenic Cre mouse line for the study of hippocampal development and adult neurogenesis.
abstract: The hippocampus is crucial for higher brain functions, such as learning, memory, and emotion. Many diseases like epilepsy and Down's syndrome are associated with abnormalities in early hippocampal development. In addition, adult dentate neurogenesis is thought to be defective in several classes of psychiatric disorders. However, the mechanisms regulating hippocampal development and adult neurogenesis remain unclear. One of the limitations to studying these processes is the scarcity of available specific mouse tools. Here, we report an inducible transgenic Cre mouse line, Frizzled 9-CreER™, in which tamoxifen administration induces Cre recombinant. Our data show that Cre is expressed in the developing hippocampal primordium, confined to the granule cell layer at P20 and further limited to the subgranular zone in the adult dentate gyrus. Cre recombinase shows very high activity in all of these regions. Thus, this transgenic line will be a powerful tool in understanding the mechanisms of hippocampal development, adult neurogenesis, and associated diseases.
authors.affiliation: Key Laboratory of Developmental Genes and Human Diseases, MOE, Institute of Life Science, Department of Pathology, School of Medicine, Southeast University, Nanjing, Jiangsu 210009, People's Republic of China.
authors.forename: Yiping
authors.initials: Y
authors.lastname: Li
authors.forename: Chuanxi
authors.initials: C
authors.lastname: Tian
authors.forename: Ying
authors.initials: Y
authors.lastname: Yang
authors.forename: Yan
authors.initials: Y
authors.lastname: Yan
authors.forename: Yang
authors.initials: Y
authors.lastname: Ni
authors.forename: Yiquan
authors.initials: Y
authors.lastname: Wei
authors.forename: Samuel J
authors.initials: SJ
authors.lastname: Pleasure
authors.forename: Chunjie
authors.initials: C
authors.lastname: Zhao
mesh_terms: D000818:Animals; D002460:Cell Line; D018891:Dentate Gyrus / Q000254:growth & development* / Q000378:metabolism*; D051157:Frizzled Receptors; D018507:Gene Expression Regulation, Developmental; D007150:Immunohistochemistry; D019426:Integrases / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D055495:Neurogenesis / Q000235:genetics*; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D017981:Receptors, Neurotransmitter / Q000235:genetics / Q000378:metabolism; D013687:Telencephalon / Q000378:metabolism
chemical_list: D051157:Frizzled Receptors; C403212:Fzd9 protein, mouse; D017981:Receptors, Neurotransmitter; C045073:Cre recombinase; D019426:Integrases