title: Production and properties of two collagenases from bacteria and their application for collagen extraction.
abstract: Two collagenolytic protease (collagenase) producing bacteria, a Gram positive Bacillus cereus CNA1 and a Gram negative Klebsiella pneumoniae CNL3, were isolated under alkaline and acidic conditions, respectively. The production of collagenase by these two bacteria was optimized. Glycerol was the suitable carbon source for collagenase production by both strains. The optimal initial pH values for collagenase production by CNA1 and CNL3 were 7.5 and 6.0, respectively, and the optimal temperature was 37°C for both strains. The maximum activity of the partially purified collagenase from CNA1 was at pH 7.0 and 45°C. Its pH and thermal stability were in the range of 6-8 and below 40°C, respectively. The maximum activity of the partially purified collagenase from CNL3 was at pH 6.0 and 40°C. Its pH and thermal stability were in the range of 5-7 and below 37°C, respectively. The collagenase from CNL3 was more stable at a low pH compared with that from CNA1. Collagenases from both strains were used to extract collagen from salmon fish skin. The use of collagenases from CNA1 and CNL3 combined with acid treatment yielded a high collagen extraction of 54.6% and 53.0%, of the fish skin dry weight, respectively.
authors.affiliation: Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Hat-Yai 90112, Thailand.
authors.forename: Warinda
authors.initials: W
authors.lastname: Suphatharaprateep
authors.forename: Benjamas
authors.initials: B
authors.lastname: Cheirsilp
authors.forename: Akkasit
authors.initials: A
authors.lastname: Jongjareonrak
mesh_terms: D000818:Animals; D001409:Bacillus cereus / Q000201:enzymology* / Q000254:growth & development; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification; D003094:Collagen / Q000737:chemistry*; D017364:Collagenases / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007711:Klebsiella pneumoniae / Q000201:enzymology* / Q000254:growth & development; D012474:Salmon; D012867:Skin / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; D003094:Collagen; D017364:Collagenases