title: Molecular epidemiology of Oropouche virus, Brazil.
abstract: Oropouche virus (OROV) is the causative agent of Oropouche fever, an urban febrile arboviral disease widespread in South America, with >30 epidemics reported in Brazil and other Latin American countries during 1960-2009. To describe the molecular epidemiology of OROV, we analyzed the entire N gene sequences (small RNA) of 66 strains and 35 partial Gn (medium RNA) and large RNA gene sequences. Distinct patterns of OROV strain clustered according to N, Gn, and large gene sequences, which suggests that each RNA segment had a different evolutionary history and that the classification in genotypes must consider the genetic information for all genetic segments. Finally, time-scale analysis based on the N gene showed that OROV emerged in Brazil ≈223 years ago and that genotype I (based on N gene data) was responsible for the emergence of all other genotypes and for virus dispersal.
authors.affiliation: Instituto Evandro Chagas, Ananindeua, Brazil.
authors.forename: Helena Baldez
authors.initials: HB
authors.lastname: Vasconcelos
authors.forename: Márcio R T
authors.initials: MR
authors.lastname: Nunes
authors.forename: Lívia M N
authors.initials: LM
authors.lastname: Casseb
authors.forename: Valéria L
authors.initials: VL
authors.lastname: Carvalho
authors.forename: Eliana V
authors.initials: EV
authors.lastname: Pinto da Silva
authors.forename: Mayra
authors.initials: M
authors.lastname: Silva
authors.forename: Samir M M
authors.initials: SM
authors.lastname: Casseb
authors.forename: Pedro F C
authors.initials: PF
authors.lastname: Vasconcelos
mesh_terms: D000818:Animals; D001938:Brazil / Q000453:epidemiology; D002044:Bunyaviridae Infections / Q000453:epidemiology* / Q000821:virology*; D002522:Chlorocebus aethiops; D019143:Evolution, Molecular; D005814:Genes, Viral / Q000235:genetics; D014644:Genetic Variation / Q000235:genetics; D005838:Genotype; D006801:Humans; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D016851:Orthobunyavirus / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D014709:Vero Cells
chemical_list: D012367:RNA, Viral