title: Synthesis of a novel zwitterionic biodegradable poly (α,β-L-aspartic acid) derivative with some L-histidine side-residues and its resistance to non-specific protein adsorption.
abstract: A novel zwitterionic polypeptide derivative, denoted as His-PAsp/PAsp, was successfully synthesized by amidation of Poly (α,β-L-aspartic acid) with L-histidine methyl ester. Turbidity, zeta potential and ¹H NMR measurements were used to study the aggregation behaviors of His-PAsp/PAsp under different pH values. The modified polypeptide derivative composed of electro-negatively carboxylic and electro-positively imidazole residues randomly so as to bear opposite charges at pH values above or below the isoelectric point. When the zwitterionic polypeptide was coated on silicon wafer as a model substrate material, the absorption resistance of fibrinogen, a blood protein resulting in the blood coagulation cascade, on the coated surface was measured. It was found that the adsorption amount of fibrinogen on the polypeptide-coated surface depended on the dose of the polypeptide on silicon wafer. Obvious resistance of the fibrinogen adsorption on the polypeptide-coated surface was observed. Since its good biodegradability and superior anti-protein-fouling property, this pH-responsive zwitterionic polypeptide is a promising candidate for surface modification in many biomedical applications, including medical implants, drug delivery carriers, and biosensors.
authors.affiliation: The Key Laboratory of Functional Polymer Materials (Ministry of Eduction), Institute of Polymer Chemistry, Nankai University, Tianjin 300071, PR China.
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Wang
authors.forename: Guolin
authors.initials: G
authors.lastname: Wu
authors.forename: Caicai
authors.initials: C
authors.lastname: Lu
authors.forename: Yinong
authors.initials: Y
authors.lastname: Wang
authors.forename: Yunge
authors.initials: Y
authors.lastname: Fan
authors.forename: Hui
authors.initials: H
authors.lastname: Gao
authors.forename: Jianbiao
authors.initials: J
authors.lastname: Ma
mesh_terms: D000327:Adsorption; D006639:Histidine / Q000737:chemistry*; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*
chemical_list: D010455:Peptides; D011506:Proteins; C017645:polyaspartate; D006639:Histidine