title: Bisphenol-A (BPA) affects reproductive formation across generations in mice.
abstract: To understand effects of Bisphenol-A (BPA) exposure on the reproductive organ across generations, we analyzed morphology of the uterus and ovary, and the methylation pattern of HOXA10 gene of the 2(nd) generation. Pregnant mice (F0) were treated with sc injection of BPA in sesame oil at various doses of 0-1,000 mg/kg Bwt on days 12-16 of gestation. Their offspring (F1) were bred by foster mice, and the offspring (F2) from F1 mice were prepared. That is, F1 mice experienced in utero BPA exposure during the developmental period of reproductive organs, while F2 mice did not at all. Using these F2 mice, the present study was carried out. Comparing to the control, the body weights in BPA exposure groups were significantly increased. Correlating with the increase of body weight, the relative weights of the ovary and uterus in each group were decreased. The histological analysis revealed expansion or emphraxis of the uterine lumen and partial loss of the uterine epithelium. Unmethylation of HOXA10 gene in the uterus was observed in the intron region. The present study suggested that BPA exposure to F0 mice could affect reproductive organ of F2 mice who were not exposed to BPA.
authors.affiliation: Laboratory of Basic Veterinary Science, The United Graduate School of Veterinary Science, Yamaguchi University, 1677–1 Yoshida, Yamaguchi 753–8515, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Hiyama
authors.forename: Ehn-Kyong
authors.initials: EK
authors.lastname: Choi
authors.forename: Shoichi
authors.initials: S
authors.lastname: Wakitani
authors.forename: Toru
authors.initials: T
authors.lastname: Tachibana
authors.forename: Hamayun
authors.initials: H
authors.lastname: Khan
authors.forename: Ken Takeshi
authors.initials: KT
authors.lastname: Kusakabe
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Kiso
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds; D001835:Body Weight; D019175:DNA Methylation; D004305:Dose-Response Relationship, Drug; D004785:Environmental Pollutants / Q000633:toxicity; D004968:Estrogens, Non-Steroidal / Q000633:toxicity*; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology; D000080905:Homeobox A10 Proteins; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D018811:Maternal Exposure; D051379:Mice; D009929:Organ Size; D010053:Ovary / Q000002:abnormalities* / Q000187:drug effects; D010636:Phenols / Q000633:toxicity*; D011247:Pregnancy; D014599:Uterus / Q000002:abnormalities* / Q000187:drug effects; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D004785:Environmental Pollutants; D004968:Estrogens, Non-Steroidal; D000080905:Homeobox A10 Proteins; D018398:Homeodomain Proteins; D010636:Phenols; C092119:Hoxa10 protein, mouse; C006780:bisphenol A; D000098826:Bisphenol A Compounds