title: Crystallization and preliminary X-ray crystallographic studies of an oligomeric species of a refolded C39 peptidase-like domain of the Escherichia coli ABC transporter haemolysin B.
abstract: The ABC transporter haemolysin B (HlyB) from Escherichia coli is part of a type I secretion system that translocates a 110 kDa toxin in one step across both membranes of this Gram-negative bacterium in an ATP-dependent manner. Sequence analysis indicates that HlyB contains a C39 peptidase-like domain at its N-terminus. C39 domains are thiol-dependent peptidases that cleave their substrates after a GG motif. Interestingly, the catalytically invariant cysteine is replaced by a tyrosine in the C39-like domain of HlyB. Here, the overexpression, purification and crystallization of the isolated C39-like domain are described as a first step towards obtaining structural insights into this domain and eventually answering the question concerning the function of a degenerated C39 domain in the ABC transporter HlyB.
authors.affiliation: Institute of Biochemistry, Heinrich Heine University Düsseldorf, Universitätsstrasse 1, D-40225 Düsseldorf, Germany.
authors.forename: Christian K W
authors.initials: CK
authors.lastname: Schwarz
authors.forename: Britta
authors.initials: B
authors.lastname: Tschapek
authors.forename: Thorsten
authors.initials: T
authors.lastname: Jumpertz
authors.forename: Stefan
authors.initials: S
authors.lastname: Jenewein
authors.forename: Justin
authors.initials: J
authors.lastname: Lecher
authors.forename: Dieter
authors.initials: D
authors.lastname: Willbold
authors.forename: Santosh
authors.initials: S
authors.lastname: Panjikar
authors.forename: I Barry
authors.initials: IB
authors.lastname: Holland
authors.forename: Sander H J
authors.initials: SH
authors.lastname: Smits
authors.forename: Lutz
authors.initials: L
authors.lastname: Schmitt
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry*; D002352:Carrier Proteins / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000737:chemistry*; D006460:Hemolysin Proteins / Q000737:chemistry*; D055503:Protein Multimerization; D058849:Protein Refolding
chemical_list: D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D002352:Carrier Proteins; D006460:Hemolysin Proteins; C050837:Hlyb protein, Bacteria