title: Multiplex PCR assay for identification of three major pathogenic Vibrio spp., Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus.
abstract: A multiplex PCR assay was developed based on atpA-sequence diversification for molecular identification of 3 major pathogenic Vibrio species: Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus. It specifically identified them from among 133 strains of various Vibrio species and other genera, and was applicable for testing seawater, suggesting its usefulness.
authors.affiliation: Department of Bacteriology I, National Institute of Infectious Diseases, Toyama, Shinjuku-ku, Tokyo, Japan. izumiya@nih.go.jp
authors.forename: Hidemasa
authors.initials: H
authors.lastname: Izumiya
authors.forename: Kazutoshi
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Yahiro
authors.forename: Jiyoung
authors.initials: J
authors.lastname: Lee
authors.forename: Masatomo
authors.initials: M
authors.lastname: Morita
authors.forename: Shouji
authors.initials: S
authors.lastname: Yamamoto
authors.forename: Eiji
authors.initials: E
authors.lastname: Arakawa
authors.forename: Makoto
authors.initials: M
authors.lastname: Ohnishi
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D014733:Vibrio / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D014734:Vibrio cholerae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D014736:Vibrio parahaemolyticus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D041261:Vibrio vulnificus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification