title: Gene dosage imbalance of human chromosome 21 in mouse embryonic stem cells differentiating to neurons.
abstract: Gene dosage imbalance is the central working hypothesis in understanding cognitive impairment and learning difficulty in Down syndrome. A mouse embryonic stem cell line containing single human chromosome 21 was used to study genomic and transcriptomic implications of autosomal imbalance during early neurogenesis. In this study bioinformatic analysis in the differentiating aneuploid neurons showed 53.6% primary dosage, 25% dosage compensation, and 21.4% reverse dosage effects on trisomic genes, revealing locus-specific secondary dosage effects on disomic genes and its specific trans-acting networks for neural attenuation, degeneration and apoptosis. The obtained results supported the significant gene dosage effects of autosomal imbalance on early neural development, suggesting novel molecular regulations for neurodevelopmental abnormalities in Down syndrome.
authors.affiliation: Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Shatin, New Territories.
authors.forename: Chi Chiu
authors.initials: CC
authors.lastname: Wang
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kazuki
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Oshimura
authors.forename: Kazuho
authors.initials: K
authors.lastname: Ikeo
authors.forename: Takashi
authors.initials: T
authors.lastname: Gojobori
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D002891:Chromosomes, Human, Pair 21; D019295:Computational Biology; D004314:Down Syndrome / Q000235:genetics*; D053595:Embryonic Stem Cells / Q000502:physiology*; D018628:Gene Dosage; D006801:Humans; D051379:Mice; D055495:Neurogenesis; D009474:Neurons / Q000502:physiology*; D014314:Trisomy