title: [Study on the best concentration of combination of the effects of the four original constituents of liu-wei di-huang pill on the proliferation and differentiation of rat preadipocyte].
abstract: OBJECTIVE
  To investigate the effects of four original constituents (Loganin, Morroniside, Peoniflorin, Paeonol) of Liu-wei Di-huang pills on the proliferation and differentiation of rat preadipocyte, and optimize the best concentration by the orthogonal test.
  
  
  METHODS
  The rat preadipocytes were cultured, then above four original constituents according to 4 factors and 3 levels to make L9 (3(4)) orthogonal test, and the control was performed at the same time. The proliferation of preadipocytes was determined by MTT method, and the accumulation of cellular lipid was determined by Oil Red O staining, the differences between factors and variances were compared by the value of absorbance.
  
  
  RESULTS
  The combination of four original constituents of Liu-wei Di-huang pills could stimulate rat preadipocyte proliferation and inhibited its lipid accumulation. Both the effects were highly significant (P < 0.01) with the combination of A3 B1 C3 D2; Compared with the monosomic group, the effects of the A3 B1 C3 D2 was highly significant (P < 0.01).
  
  
  CONCLUSIONS
  Compared with the monosomic group, the effects of the combination of the four original constituents of Liu-wei Di-huang pills on the proliferation and differentiation of rat preadipocyte were highly significant. This combination consists of Loganin, morroniside with high doses, Paeonol, Peoniflorin with mid-dose and low-dose, respectively. Regnesent the overall regulatory role of synergy and officiencg.
authors.affiliation: The First Affiliated Hospial of Hunan Uninevsity of TCM, Changsha 410208, China. db0223@163.com
authors.forename: Bing
authors.initials: B
authors.lastname: Dai
authors.forename: Xing-Feng
authors.initials: XF
authors.lastname: Li
authors.forename: Zi-Zeng
authors.initials: ZZ
authors.lastname: Xiao
authors.forename: Ke-Jia
authors.initials: KJ
authors.lastname: Shen
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.forename: Liu
authors.initials: L
authors.lastname: Peng
mesh_terms: D000098:Acetophenones / Q000008:administration & dosage / Q000494:pharmacology; D017667:Adipocytes / Q000166:cytology* / Q000187:drug effects; D000818:Animals; D001565:Benzoates / Q000008:administration & dosage / Q000494:pharmacology; D001952:Bridged-Ring Compounds / Q000008:administration & dosage / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004338:Drug Combinations; D004357:Drug Synergism; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D005223:Fats / Q000378:metabolism; D005960:Glucosides / Q000008:administration & dosage / Q000494:pharmacology; D006027:Glycosides / Q000008:administration & dosage / Q000494:pharmacology; D039823:Iridoids / Q000008:administration & dosage / Q000494:pharmacology; D008297:Male; D039821:Monoterpenes; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000098:Acetophenones; D001565:Benzoates; D001952:Bridged-Ring Compounds; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D005223:Fats; D005960:Glucosides; D006027:Glycosides; D039823:Iridoids; C042579:Liuwei Dihuang Decoction; D039821:Monoterpenes; C488401:morroniside; C015423:peoniflorin; C013638:paeonol; C059516:loganin