title: Brassinosteroid enhances jasmonate-induced anthocyanin accumulation in Arabidopsis seedlings.
abstract: Jasmonate (JA) regulates plant development, mediates defense responses, and induces anthocyanin biosynthesis as well. Previously, we isolated the psc1 mutant that partially suppressed coi1 insensitivity to JA, and found that brassinosteroid (BR) was involved in JA signaling and negatively regulated JA inhibition of root growth in Arabidopsis. In this study it was shown that JA-induced anthocyanin accumulation was reduced in BR mutants or in wild type treated with brassinazole, an inhibitor of BR biosynthesis, whereas it was induced by an application of exogenous BR. It was also shown that the 'late' anthocyanin biosynthesis genes including DFR, LDOX, and UF3GT, were induced slightly by JA in the BR mutants relative to wild type. Furthermore, the expression level of JA-induced Myb/bHLH transcription factors such as PAP1, PAP2, and GL3, which are components of the WD-repeat/Myb/bHLH transcriptional complexes that mediate the 'late' anthocyanin biosynthesis genes, was lower in the BR mutants than that in wild type. These results suggested that BR affects JA-induced anthocyanin accumulation by regulating the 'late' anthocyanin biosynthesis genes and this regulation might be mediated by the WD-repeat/Myb/bHLH transcriptional complexes.
authors.affiliation: Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization, Hunan Agricultural University, Changsha, China.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Peng
authors.forename: Chengyun
authors.initials: C
authors.lastname: Han
authors.forename: Libing
authors.initials: L
authors.lastname: Yuan
authors.forename: Kun
authors.initials: K
authors.lastname: Zhang
authors.forename: Hongman
authors.initials: H
authors.lastname: Huang
authors.forename: Chunmei
authors.initials: C
authors.lastname: Ren
mesh_terms: D000872:Anthocyanins / Q000378:metabolism*; D017360:Arabidopsis / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism; D060406:Brassinosteroids / Q000494:pharmacology*; D003517:Cyclopentanes / Q000494:pharmacology*; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant / Q000235:genetics; D009154:Mutation / Q000235:genetics; D054883:Oxylipins / Q000494:pharmacology*; D000073718:Pancreatitis-Associated Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction; D036226:Seedlings / Q000187:drug effects* / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D013260:Steroids, Heterocyclic / Q000494:pharmacology*; D014230:Triazoles / Q000494:pharmacology
chemical_list: D000872:Anthocyanins; D029681:Arabidopsis Proteins; D051792:Basic Helix-Loop-Helix Proteins; D060406:Brassinosteroids; D003517:Cyclopentanes; D003577:Cytochrome P-450 Enzyme System; D054883:Oxylipins; D000073718:Pancreatitis-Associated Proteins; D013260:Steroids, Heterocyclic; D014230:Triazoles; C111451:AT3G50660 protein, Arabidopsis; C000612141:REG3A protein, human; C011006:jasmonic acid; C412342:brassinazole; C023623:brassinolide