title: Expression of CD44 spliced variants with both exons v9 and v10 in primary N(-)M(-) breast carcinomas correlates with metastasis.
abstract: We analyzed the representation of CD44 isoforms with both exons v9 and v10 among CD44 total amount and also examined correlation between their expression, clinical parameters and survival. We used a semi-quantitative RT-PCR reaction and a panel of 25 normal breast specimens, 10 mammary fibroadenomas, 8 cystic samples and 52 primary breast tumors. CD44 expression level was statistically higher in malignant tumors than in normal breast tissues (p = 0.038) or in fibroadenomas (p = 0.047) and correlated with histological grading, p = 0.047. Ratios CD44 variants with both exons v9 and v10/ total CD44 were similar in normal breast tissues and fibroadenomas but lower in the cystic samples. In primary N(-)M(-) breast tumors, unfavourable outcome and relapse were correlated with low ratios.
authors.affiliation: UNIV HOSP,INST RECH HEMATOL,F-67000 STRASBOURG,FRANCE. UNIV HOSP,DEPT GYNAECOL,F-67000 STRASBOURG,FRANCE. UNIV HOSP,DEPT STOMATOL & PLAST SURG,F-67000 STRASBOURG,FRANCE. UNIV HOSP,DEPT ONCOL,F-67000 STRASBOURG,FRANCE. UNIV HOSP,DEPT ANATOMOPATHOL,F-67000 STRASBOURG,FRANCE. UNIV HOSP,DEPT SURG ONCOL,F-67000 STRASBOURG,FRANCE.
authors.forename: N
authors.initials: N
authors.lastname: Guriec
authors.forename: B
authors.initials: B
authors.lastname: Gairard
authors.forename: L
authors.initials: L
authors.lastname: Marcellin
authors.forename: A
authors.initials: A
authors.lastname: Wilk
authors.forename: H
authors.initials: H
authors.lastname: Calderoli
authors.forename: R
authors.initials: R
authors.lastname: Renaud
authors.forename: J
authors.initials: J
authors.lastname: Bergerat
authors.forename: F
authors.initials: F
authors.lastname: Oberling