title: Laser confers less embryo exposure than acid tyrode for embryo biopsy in preimplantation genetic diagnosis cycles: a randomized study.
abstract: We compared two methods of zona pellucida drilling. 213 embryos were biopsied with acid Tyrode. Each biopsy took 3 minutes and the entire procedure ~29 minutes. 5% of blastomeres lysed, 49% of embryos became blastocyst and 36% of patients became pregnant. 229 embryos were biopsied with laser. Each biopsy took 30 seconds and the entire procedure ~7 minutes. 2.5% of blastomeres lysed, 50.6% of embryos became blastocyst and 47% of patients became pregnant. We can conclude that laser can be used for embryo biopsy. Reduction of embryo exposure and of removed blastomeres is associated with increased blastocysts available for transfer and a better clinical outcome.
authors.affiliation: ORIGEN, Centro de Medicina Reprodutiva, Av Contorno 7747, Lourdes, CEP 30110-120 Belo Horizonte, Minas Gerais, Brazil. selmogeber@origen.com.br
authors.forename: Selmo
authors.initials: S
authors.lastname: Geber
authors.forename: Renata
authors.initials: R
authors.lastname: Bossi
authors.forename: Cintia B
authors.initials: CB
authors.lastname: Lisboa
authors.forename: Marcelo
authors.initials: M
authors.lastname: Valle
authors.forename: Marcos
authors.initials: M
authors.lastname: Sampaio
mesh_terms: D000328:Adult; D001706:Biopsy / Q000379:methods*; D004622:Embryo, Mammalian / Q000187:drug effects* / Q000473:pathology; D047108:Embryonic Development / Q000187:drug effects; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007552:Isotonic Solutions; D007834:Lasers; D011247:Pregnancy; D018873:Pregnancy Rate; D019836:Preimplantation Diagnosis / Q000379:methods*; D020554:Sperm Injections, Intracytoplasmic; D015044:Zona Pellucida / Q000187:drug effects / Q000473:pathology
chemical_list: D007552:Isotonic Solutions; C037692:Tyrode's solution