title: Tumoricidal function against human cervical-cancer cells in human peritoneal mesothelial cells - contribution of tumor-necrosis-factor-alpha.
abstract: The present study demonstrates the important and novel finding that human mesothelial cells possess tumoricidal activity. This cytotoxicity in mesothelial cells was induced by recombinant human interferon (rhIFN)-gamma and was measured against the human cervical carcinoma cell line, ME-180, by Cr-51-release, visual assessment and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide uptake assays. Tumor necrosis factor (TNF)-alpha-sensitive ME-180 cells were shown to be lysed by 24-h-rhIFN-gamma-treated-mesothelial cell-supernatants alone, which was partially inhibitable by anti-TNF-alpha. Induction of TNF-alpha in mesothelial cells was confirmed by Northern blotting. Our data describe a novel rhIFN-gamma-activated cytotoxic function in mesothelial cells, and suggest the involvement of TNF-alpha as one of the lytic mechanisms. These results are of extreme importance in relation to the future understanding of general local carcinogenic processes, and henceforth therapies.
authors.affiliation: UNIV S FLORIDA,COLL MED,DEPT BIOCHEM,TAMPA,FL 33612. UNIV S FLORIDA,COLL MED,DEPT MED MICROBIOL & IMMUNOL,H LEE MOFFITT CANC CTR & RES INST,TAMPA,FL 33612. UNIV S FLORIDA,COLL MED,DEPT OBSTET & GYNECOL,DIV GYNECOL ONCOL,TAMPA,FL 33612.
authors.forename: M
authors.initials: M
authors.lastname: Michelininorris
authors.forename: D
authors.initials: D
authors.lastname: Blanchard
authors.forename: D
authors.initials: D
authors.lastname: Barton
authors.forename: W
authors.initials: W
authors.lastname: Roberts
authors.forename: D
authors.initials: D
authors.lastname: Cavanagh
authors.forename: J
authors.initials: J
authors.lastname: Djeu