title: Simultaneous production of citric acid and invertase by Yarrowia lipolytica SUC+ transformants.
abstract: Simultaneous production of citric acid (CA) and invertase by Yarrowia lipolytica A-101-B56-5 (SUC(+) clone) growing from sucrose, mixture of glucose and fructose, glucose or glycerol was investigated. Among the tested substrates the highest concentration of CA was reached from glycerol (57.15 g/L) with high yield (Y(CA/S)=0.6g/g). When sucrose was used, comparable amount of CA was secreted (45 g/L) with slightly higher yield (Y(CA/S)=0.643 g/g). In all cultures amount of isocitrate (ICA) was below 2% of total citrates. Considering invertase production, the best carbon source appeared to be sucrose (72380 U/L). The highest yield of CA and invertase biosynthesis calculated for 1g of biomass was obtained for cells growing from glycerol (9.9 g/g and 4325 U/g, respectively). Concentrates of extra- and intracellular invertase of the highest activity were obtained from sucrose as substrate (0.5 and 1.8 × 10(6)U/L, respectively).
authors.affiliation: Department of Biotechnology and Food Microbiology, Wroclaw University of Environmental and Life Sciences, Norwida 25/27, 50-375 Wroclaw, Poland.
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Lazar
authors.forename: Ewa
authors.initials: E
authors.lastname: Walczak
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Robak
mesh_terms: D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D019343:Citric Acid / Q000378:metabolism*; D005285:Fermentation / Q000502:physiology; D005632:Fructose / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal / Q000235:genetics; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D042541:Intracellular Space / Q000201:enzymology; D007523:Isocitrates / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism*; D014170:Transformation, Genetic; D025062:Yarrowia / Q000235:genetics* / Q000378:metabolism*; D043324:beta-Fructofuranosidase / Q000096:biosynthesis*
chemical_list: D007523:Isocitrates; D019343:Citric Acid; D005632:Fructose; D013395:Sucrose; C034219:isocitric acid; D043324:beta-Fructofuranosidase; D005947:Glucose; D005990:Glycerol