title: [Isolating sperm cells by laser capture microdissection technique from mixture sample].
abstract: OBJECTIVE
  To assess the application value of laser capture microdissection (LCM) technique for isolating a small number of sperm cells from mixture sample.
  
  
  METHODS
  Mixture samples were prepared with sperm cells and vaginal epithelia at different concentrations. Both LCM technique and the differential lysis method were employed to obtain sperm cells from the mixture samples, and DNA was extracted by magnetic beads method. STR genotyping was determined using Identifiler kit.
  
  
  RESULTS
  The successful STR genotype rate of sperm cells isolated from mixture samples with LCM technique was 92.86% (13/14). The rate of differential lysis method was 7.14% (1/14). The successful rates between the two methods were statistically different (P < 0.05).
  
  
  CONCLUSIONS
  LCM technique can effectively exclude the interference of female cell component and isolate a small number of sperm cells to obtain a single male STR genotyping. LCM technique is obviously better than the differential lysis method.
authors.affiliation: School of Forensic Medicine, Shanxi Medical University, Taiyuan 030001, China. lf0911@qq.com
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Li-juan
authors.initials: LJ
authors.lastname: Yu
authors.forename: Jian-zhang
authors.initials: JZ
authors.lastname: Guo
authors.forename: Jun-wei
authors.initials: JW
authors.lastname: Gao
authors.forename: Zhang-ping
authors.initials: ZP
authors.lastname: Jiao
authors.forename: Hui
authors.initials: H
authors.lastname: Tang
mesh_terms: D002469:Cell Separation / Q000379:methods*; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D016172:DNA Fingerprinting / Q000379:methods; D004847:Epithelial Cells; D005260:Female; D005554:Forensic Medicine / Q000379:methods; D005838:Genotype; D006801:Humans; D061346:Laser Capture Microdissection / Q000379:methods*; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods; D013094:Spermatozoa / Q000166:cytology*; D013194:Staining and Labeling; D020080:Tandem Repeat Sequences; D014621:Vagina / Q000166:cytology
chemical_list: D004247:DNA