title: Modification of buffered peptone water for improved recovery of heat-injured Salmonella Typhimurium.
abstract: Rapid detection of Salmonella in foods is often limited by the high demand for the sensitivity of detection, poor physiological conditions of the target cells, and high concentration of background flora. In this study, the conditions of nonselective enrichment cultivation were modified in order to improve the quantitative detection of heat-injured Salmonella in minced meat. The effect of the modifications on the recovery was observed by means of RNA-based sandwich hybridization, which was adjusted for the quantification of Salmonella enterica 23S rRNA in crude cell extracts. The supplementation of buffered peptone water with the enzyme-controlled substrate delivery system EnBase-Flo and ferrioxamine E was shown to improve the recovery of cells in both single strain cultures and in the presence of minced meat. The presented results can be used for the development of more efficient enrichment cultivation media for faster detection of food borne Salmonella.
authors.affiliation: Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering, University of Oulu, P.O. Box 4300, Oulu FI-90014, Finland. sanna.taskila@oulu.fi
authors.forename: Sanna
authors.initials: S
authors.lastname: Taskila
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Osmekhina
authors.forename: Mika
authors.initials: M
authors.lastname: Tuomola
authors.forename: Jari
authors.initials: J
authors.lastname: Ruuska
authors.forename: Peter
authors.initials: P
authors.lastname: Neubauer
mesh_terms: D015373:Bacterial Typing Techniques; D002470:Cell Survival / Q000187:drug effects; D015169:Colony Count, Microbial; D003470:Culture Media / Q000737:chemistry*; D005290:Ferric Compounds / Q000494:pharmacology; D005516:Food Microbiology / Q000379:methods*; D006358:Hot Temperature / Q000009:adverse effects*; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents / Q000494:pharmacology*; D008461:Meat Products / Q000382:microbiology; D015233:Models, Statistical; D009693:Nucleic Acid Hybridization; D010456:Peptides, Cyclic / Q000494:pharmacology; D010461:Peptones / Q000378:metabolism*; D012329:RNA, Bacterial / Q000378:metabolism; D012338:RNA, Ribosomal, 23S / Q000378:metabolism; D012486:Salmonella typhimurium / Q000145:classification / Q000187:drug effects* / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D003470:Culture Media; D005290:Ferric Compounds; D007202:Indicators and Reagents; D010456:Peptides, Cyclic; D010461:Peptones; D012329:RNA, Bacterial; D012338:RNA, Ribosomal, 23S; C017508:ferrioxamine E