title: Characterization of a human cell line stably over-expressing the candidate oncogene, dual specificity phosphatase 12.
abstract: BACKGROUND
  Analysis of chromosomal rearrangements within primary tumors has been influential in the identification of novel oncogenes. Identification of the "driver" gene(s) within cancer-derived amplicons is, however, hampered by the fact that most amplicons contain many gene products. Amplification of 1q21-1q23 is strongly associated with liposarcomas and microarray-based comparative genomic hybridization narrowed down the likely candidate oncogenes to two: the activating transcription factor 6 (atf6) and the dual specificity phosphatase 12 (dusp12). While atf6 is an established transcriptional regulator of the unfolded protein response, the potential role of dusp12 in cancer remains uncharacterized.
  
  
  RESULTS
  To evaluate the oncogenic potential of dusp12, we established stable cell lines that ectopically over-express dusp12 in isolation and determined whether this cell line acquired properties frequently associated with transformed cells. Here, we demonstrate that cells over-expressing dusp12 display increased cell motility and resistance to apoptosis. Additionally, over-expression of dusp12 promoted increased expression of the c-met proto-oncogene and the collagen and laminin receptor intergrin alpha 1 (itga1) which is implicated in metastasis.
  
  
  CONCLUSIONS
  Collectively, these results suggest that dusp12 is oncologically relevant and exposes a potential association between dusp12 and established oncogenes that could be therapeutically targeted.
authors.affiliation: Division of Biology, Kansas State University, Manhattan, Kansas, United States of America.
authors.forename: Erica L
authors.initials: EL
authors.lastname: Cain
authors.forename: Sterling E
authors.initials: SE
authors.lastname: Braun
authors.forename: Alexander
authors.initials: A
authors.lastname: Beeser
mesh_terms: D017209:Apoptosis / Q000502:physiology; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology; D054637:Dual-Specificity Phosphatases / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D006801:Humans; D008854:Microscopy, Electron; D009857:Oncogenes; D000090063:Proto-Oncogene Mas; D012333:RNA, Messenger / Q000235:genetics
chemical_list: C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D012333:RNA, Messenger; C489855:dual specificity phosphatase 12; D054637:Dual-Specificity Phosphatases