title: [Determination of rosmarinic acid in seeds and its processed products of Perilla frutescens var. typica and Perilla frutescens var. acuta].
abstract: OBJECTIVE
  To determine the content of rosmarinic acid in seeds and its processed products of Perilla frutescens var. typica (PFT), Perilla frutescens var. acuta (PFA) and Perilla frutescens var. acuta form. discolor (PFAD).
  
  
  METHODS
  The content of rosmarinic acid was determined by RP-HPLC with Welch C18 (250 mm x 4.6 mm, 5 microm) column, using methanol-phosphonic acid solution (39:61) as mobile phase at flow rate of 1.0 mL/min. UV detection wavelength was set at 330 nm and column temperature was set at 28 degrees C.
  
  
  RESULTS
  The content of rosmarinic acid in roasted seeds of PFT is higher than in its seeds, but that of PFA and PFAD are lower; The content of rosmarinic acid in seeds of PFT, PFA and PFAD decline after the seeds are honey-pocessed and made into frost-like powder, and that of honey-processed one decline by a larger margin.
  
  
  CONCLUSIONS
  The content of rosmarinic acid in seeds of PFT, PFA and PFAD are prone to decline by heat-processing.
authors.affiliation: Biomedicine Key Laboratory of Shaanxi Province, Northwest University, Xi'an 710069, China. lianghui_huang@163.com
authors.forename: Liang-Hui
authors.initials: LH
authors.lastname: Huang
authors.forename: Qi
authors.initials: Q
authors.lastname: Su
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Zhao
authors.forename: Wen-Ji
authors.initials: WJ
authors.lastname: Sun
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002934:Cinnamates / Q000032:analysis*; D053630:Depsides / Q000032:analysis*; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification; D006358:Hot Temperature; D031344:Perilla frutescens / Q000737:chemistry*; D010598:Pharmacognosy; D011786:Quality Control; D015203:Reproducibility of Results; D012639:Seeds / Q000737:chemistry*; D013678:Technology, Pharmaceutical / Q000379:methods*; D000097123:Rosmarinic Acid
chemical_list: D002934:Cinnamates; D053630:Depsides; D004365:Drugs, Chinese Herbal; D000097123:Rosmarinic Acid