title: Association of platelet-derived growth factor receptor β accumulation with increased oxidative stress and cellular injury in sestrin 2 silenced human glioblastoma cells.
abstract: Sestrin 2 (SESN2) is a stress-inducible protein required for maintaining redox homeostasis. However, its mode of action remains to be clarified. In this study, we found that SESN2 is induced in human glioblastoma U87 cells following ionizing radiation (IR). SESN2 silencing not only results in increased oxidative stress but also sensitizes U87 cells to IR. Intriguingly, we found SESN2 silencing significantly increases the expression of platelet-derived growth factor receptor β (PDGFRβ). Using a double knockdown technique, we showed that inhibition of PDGFRβ accumulation in SESN2-silencing cells protects the cells from the deleterious effects induced by SESN2 silencing. Taken together, we revealed that PDGFRβ accumulation is associated with increased oxidative stress and cellular damage in SESN2 silenced human glioblastoma U87 cells.
authors.affiliation: Department of Biomedical Image and Radiological Sciences, National Yang-Ming University, Taipei, Taiwan, ROC.
authors.forename: Shin-Yi
authors.initials: SY
authors.lastname: Liu
authors.forename: Yi-Jang
authors.initials: YJ
authors.lastname: Lee
authors.forename: Te-Chang
authors.initials: TC
authors.lastname: Lee
mesh_terms: D045744:Cell Line, Tumor; D005909:Glioblastoma / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D009687:Nuclear Proteins / Q000235:genetics*; D018384:Oxidative Stress; D034741:RNA, Small Interfering / Q000235:genetics*; D020797:Receptor, Platelet-Derived Growth Factor beta / Q000378:metabolism*; D000084622:Sestrins
chemical_list: D009687:Nuclear Proteins; D034741:RNA, Small Interfering; D020797:Receptor, Platelet-Derived Growth Factor beta; C465398:SESN2 protein, human; D000084622:Sestrins