title: [Effects of nonylphenol and cadmium on sperm acrosome reaction in vitro in mice].
abstract: OBJECTIVE
  To study the effects of nonylphenol and cadmium on acrosome reaction in vitro in mouse spermatozoa.
  
  
  METHODS
  Sperm were collected from the vas deferens of mice, capacitated in vitro and stimulated with A23187 at 30 micromol/L to induce acrosome reaction. Then the sperm suspension was treated with nonylphenol at 10, 20, 30, 60 and 100 micromol/L or cadmium at 500, 2500 and 5 000 micromol/L, and the control group treated with the carrier solvent. Acrosome reaction of the sperm was analyzed by FITC-PSA staining.
  
  
  RESULTS
  Compared with the control group, nonylphenol significantly inhibited acrosome reaction at the concentration of > 60 micromol/L (P < 0.01), but not at < 30 micromol/L (P > 0.05), and the sperm survival rate was reduced with increased concentration of nonylphenol. However, cadmium exhibited no significant influence on either acrosome reaction (P > 0.05) or sperm survival rate at 500 - 5 000 micromol/L.
  
  
  CONCLUSIONS
  Nonylphenol and cadmium affect the spermatogenesis of mice in different ways; the former directly inhibits sperm acrosome reaction, while the latter has no direct effect on it.
authors.affiliation: College of Life Science, Capital Normal University, Beijing 100048, China. shaozhuxiong@sina.com
authors.forename: Zhu-Xiong
authors.initials: ZX
authors.lastname: Shao
authors.forename: Hong-Tao
authors.initials: HT
authors.lastname: Jiang
authors.forename: Fang
authors.initials: F
authors.lastname: Liang
authors.forename: Bao-Chang
authors.initials: BC
authors.lastname: Zhu
mesh_terms: D000177:Acrosome / Q000187:drug effects*; D020101:Acrosome Reaction / Q000187:drug effects*; D000818:Animals; D002104:Cadmium / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010636:Phenols / Q000494:pharmacology*; D013094:Spermatozoa / Q000187:drug effects
chemical_list: D010636:Phenols; D002104:Cadmium; C025256:nonylphenol