title: Postprandial lipoprotein metabolism: VLDL vs chylomicrons.
abstract: Since Zilversmit first proposed postprandial lipemia as the most common risk of cardiovascular disease, chylomicrons (CM) and CM remnants have been thought to be the major lipoproteins which are increased in the postprandial hyperlipidemia. However, it has been shown over the last two decades that the major increase in the postprandial lipoproteins after food intake occurs in the very low density lipoprotein (VLDL) remnants (apoB-100 particles), not CM or CM remnants (apoB-48 particles). This finding was obtained using the following three analytical methods; isolation of remnant-like lipoprotein particles (RLP) with specific antibodies, separation and detection of lipoprotein subclasses by gel permeation HPLC and determination of apoB-48 in fractionated lipoproteins by a specific ELISA. The amount of the apoB-48 particles in the postprandial RLP is significantly less than the apoB-100 particles, and the particle sizes of apoB-48 and apoB-100 in RLP are very similar when analyzed by HPLC. Moreover, CM or CM remnants having a large amount of TG were not found in the postprandial RLP. Therefore, the major portion of the TG which is increased in the postprandial state is composed of VLDL remnants, which have been recognized as a significant risk for cardiovascular disease.
authors.affiliation: School of Health Sciences, Faculty of Medicine, Gunma University, Maebashi, Gunma, Japan. nakajimak05@ybb.ne.jp
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Nakajima
authors.forename: Takamitsu
authors.initials: T
authors.lastname: Nakano
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Tokita
authors.forename: Takeaki
authors.initials: T
authors.lastname: Nagamine
authors.forename: Akihiro
authors.initials: A
authors.lastname: Inazu
authors.forename: Junji
authors.initials: J
authors.lastname: Kobayashi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Mabuchi
authors.forename: Kimber L
authors.initials: KL
authors.lastname: Stanhope
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Havel
authors.forename: Mitsuyo
authors.initials: M
authors.lastname: Okazaki
authors.forename: Masumi
authors.initials: M
authors.lastname: Ai
authors.forename: Akira
authors.initials: A
authors.lastname: Tanaka
mesh_terms: D000818:Animals; D002914:Chylomicrons / Q000097:blood / Q000378:metabolism*; D006801:Humans; D008079:Lipoproteins, VLDL / Q000097:blood / Q000378:metabolism*
chemical_list: D002914:Chylomicrons; D008079:Lipoproteins, VLDL