title: Interactive effects of insulin, IGF-I and GMl ganglioside on growth of PC12 cells.
abstract: PC12 cells grow in serum-free medium (SFM) if high doses of insulin (5-50 μg/ml), or combined insulin (5 μg/ml) and insulin-like growth factor-I (3 ng/ml) are added daily. Ganglioside GMl counteracts these effects in a dose-responsive fashion between 10 nM and 50 μM. This provides a serum-free system to study the interactive effects of gangliosides on growth and neuritogenesis in PC12 cells.
authors.affiliation: Division of Neuropathology, Department of Pathology, The Ohio State University, Columbus, OH (USA).
authors.forename: A J
authors.initials: AJ
authors.lastname: Yates
authors.forename: J
authors.initials: J
authors.lastname: Conley