title: Identification and characterization of Leucobacter sp. N-4 for Ni (II) biosorption by response surface methodology.
abstract: In the present study, batch experiments were carried out to characterize and optimize the removal process of Ni (II) by a nickel tolerant strain Leucobacter sp. N-4, which was isolated from the soil samples. The effects of operating parameters with respect to initial solution pH (3.0-6.5), initial nickel concentration (50-100mg/L) and biomass dosage (1-10 g/L) on Ni (II) biosorption were investigated by response surface methodology (RSM). The maximal Ni (II) removal efficiency (nearly 99%) was achieved under the following conditions: pH 4.75, biomass dosage 5.38 g/L and initial Ni (II) concentration 53.6 mg/L. The adsorption-equilibrium data fitted well with both Langmuir and Freundlich isotherms. The monolayer adsorption capacity of biomass obtained from Langmuir isotherm was about 19.6 mg/g. Infrared spectrometer (IR) results showed that chemical functional groups (e.g. -NH(2), -OH and COO-M) of the biomass should be the active binding sites for Ni (II) biosorption from aqueous solutions.
authors.affiliation: State Key Laboratory of Fine Chemicals, Key Laboratory of Industrial Ecology and Environmental Engineering (MOE), School of Environmental Science and Technology, Dalian University of Technology, Linggong Road 2, Dalian 116024, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Qu
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Ang
authors.initials: A
authors.lastname: Li
authors.forename: Fang
authors.initials: F
authors.lastname: Ma
authors.forename: Jiti
authors.initials: J
authors.lastname: Zhou
mesh_terms: D000327:Adsorption; D001665:Binding Sites; D001673:Biodegradation, Environmental; D018533:Biomass; D004361:Drug Tolerance; D006094:Gram-Positive Bacteria / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008722:Methods; D009532:Nickel / Q000494:pharmacology*; D012988:Soil Microbiology
chemical_list: D009532:Nickel