title: Assessment of the pathway of apoptosis involving PAR-4, DAXX and ZIPK proteins in CLL patients and its relationship with the principal prognostic factors.
abstract: Par-4 (prostate apoptosis response-4) protein was originally found upregulated in prostate tumor cells undergoing apoptosis. Then it was further identified as a proapoptotic protein upregulated both in normal and leukemic lymphocytes. The aim of our study was to assess PAR-4 protein expression in the B cells of CLL patients and to examine its relationship with the expression of other proteins involved in the apoptosis process, such as DAXX, ZIPK and BCL-2. We found a positive relationship between PAR-4 and BCL-2 protein expression. Additionally, there was a positive correlation between PAR-4 and both DAXX and ZIPK protein expression. The results of our research were also analyzed in association with the principal CLL prognostic factors. There was a positive correlation between the expression of PAR-4 protein and the lactate dehydrogenase (LDH) serum concentration (p < 0.005). The expression of PAR-4 protein in B cells correlated positively with the percentage of CD38(+) cells (p < 0.05), as well as with CD38(+)/ZAP-70(+) cells (p < 0.05). Moreover, we found a close relationship between LPL protein expression or LPL/ADAM29 MFI ratio and PAR-4 protein expression. Our results confirm the significance of apoptosis deregulation in CLL, and suggest a possible relationship between PAR-4 expression and the clinical course of the disease. This however requires further investigation.
authors.affiliation: Department of Clinical Immunology, Medical University of Lublin, Poland. agnieszkajunak@poczta.onet.pl
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Bojarska-Junak
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Sieklucka
authors.forename: Iwona
authors.initials: I
authors.lastname: Hus
authors.forename: Ewa
authors.initials: E
authors.lastname: Wąsik-Szczepanek
authors.forename: Maria Luiza
authors.initials: ML
authors.lastname: Kusz
authors.forename: Agata
authors.initials: A
authors.lastname: Surdacka
authors.forename: Sylwia
authors.initials: S
authors.lastname: Chocholska
authors.forename: Anna
authors.initials: A
authors.lastname: Dmoszyńska
authors.forename: Jacek
authors.initials: J
authors.lastname: Roliński
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000032:analysis / Q000378:metabolism*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000032:analysis / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000032:analysis / Q000378:metabolism*; D056970:Co-Repressor Proteins; D005260:Female; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000378:metabolism*; D020930:MAP Kinase Kinase Kinases / Q000032:analysis / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D018832:Molecular Chaperones; D009367:Neoplasm Staging; D009687:Nuclear Proteins / Q000032:analysis / Q000378:metabolism*; D011379:Prognosis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D051017:Apoptosis Regulatory Proteins; D014408:Biomarkers, Tumor; D056970:Co-Repressor Proteins; C106985:DAXX protein, human; D018832:Molecular Chaperones; D009687:Nuclear Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; C101296:prostate apoptosis response-4 protein; D020930:MAP Kinase Kinase Kinases; C090757:mitogen-activated protein kinase kinase kinase 12