title: The peripheral myelin protein 22 kDa (PMP22) gene is amplified in cell lines derived from glioma and osteogenic sarcoma.
abstract: Two glioma (SF188, SF763) and two osteogenic sarcoma cell lines (RH30, SA1) were examined for the presence of an amplification of the PMP22 gene by means of fluorescence in situ hybridization (FISH). In one cell line of both cell types, we found about 10 copies of the PMP22 (peripheral myelin protein 22 kDa) gene, located on different marker chromosomes within homogeneously staining regions. Surrounding chromosome 17p material was found to be coamplified, but coamplification of TP53 (17p13) and erbB2/Her2 (17q11.12) were excluded by FISH for both cell lines, SF763 and RH30. This is the first report of a PMP22 amplification in cell lines derived from human tumors.
authors.affiliation: UNIV ERLANGEN NURNBERG,INST HUMAN GENET,D-91054 ERLANGEN,GERMANY. UNIV CALIF SAN FRANCISCO,BRAIN TUMOR RES CTR,SAN FRANCISCO,CA 94143. UNIV CALIF SAN FRANCISCO,DEPT LAB MED,SAN FRANCISCO,CA 94143.
authors.forename: T
authors.initials: T
authors.lastname: Liehr
authors.forename: O
authors.initials: O
authors.lastname: Park
authors.forename: B
authors.initials: B
authors.lastname: Feuerstein
authors.forename: E
authors.initials: E
authors.lastname: Gebhart
authors.forename: B
authors.initials: B
authors.lastname: Rautenstrauss