title: Photocytotoxicity of hypocrellin B (HB) was enhanced by liposomalization in vitro.
abstract: Hypocrellin B (HB) was encapsulated into a phosphatidylcholine liposome. Encapsulation of HB into liposomes not only improved the delivery of this photosensitizer but also increased its photodynamic efficacy compared to free HB molecules. Liposomal HB showed a higher cellular uptake than free HB as measured by confocal microscopy and was internalized into cultured HeLa cells by caveolar endocytosis, which was lipid-raft-dependent. Cell viability measurements demonstrated that liposomal HB was more phototoxic to HeLa cells than free HB as a result of the higher concentration of intracellular HB delivered by the liposomal formulation. The encapsulation of HB influenced the cell death pathway by an increased rate of necrotic cells after irradiation versus free HB, and a Type II (singlet oxygen) mechanism was responsible for the photocytotoxicity.
authors.affiliation: College of Life Science and Bioengineering, Beijing University of Technology, Beijing, China. zhouzhixiang@bjut.edu.cn
authors.forename: Zhixiang
authors.initials: Z
authors.lastname: Zhou
authors.forename: Lina
authors.initials: L
authors.lastname: Zhang
authors.forename: Huimin
authors.initials: H
authors.lastname: Liu
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.forename: Boya
authors.initials: B
authors.lastname: Liu
authors.forename: Ping
authors.initials: P
authors.lastname: Ai
authors.forename: Hongtao
authors.initials: H
authors.lastname: Ma
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Zelin
authors.initials: Z
authors.lastname: Li
authors.forename: Wang
authors.initials: W
authors.lastname: Sheng
authors.forename: Yi
authors.initials: Y
authors.lastname: Zeng
authors.forename: Rugang
authors.initials: R
authors.lastname: Zhong
mesh_terms: D002470:Cell Survival; D003593:Cytoplasm; D003603:Cytotoxins / Q000493:pharmacokinetics; D016503:Drug Delivery Systems / Q000379:methods*; D004705:Endocytosis; D006367:HeLa Cells; D006801:Humans; D008081:Liposomes / Q000378:metabolism; D018613:Microscopy, Confocal; D010569:Perylene / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D010713:Phosphatidylcholines / Q000378:metabolism; D010778:Photochemotherapy / Q000379:methods; D017319:Photosensitizing Agents / Q000493:pharmacokinetics*; D011809:Quinones / Q000493:pharmacokinetics*
chemical_list: D003603:Cytotoxins; D008081:Liposomes; D010713:Phosphatidylcholines; D017319:Photosensitizing Agents; D011809:Quinones; D010569:Perylene; C064522:hypocrellin B