title: Application of renewable silver amalgam annular band electrode to voltammetric determination of vitamins C, B1 and B2.
abstract: In this work, the design and results of applying silver liquid amalgam film-modified silver solid amalgam annular band electrode (AgLAF-AgSAE), refreshed before each measurement, to voltammetric determination of vitamins C (VC), B(1) (VB1) and B(2) (VB2) are presented. The method is based on adsorptive accumulation of analytes at the AgLAF-AgSAE in a phosphate buffer (VB1), phosphate buffer with Triton X-100 (VB2) and an alkaline borate buffer with Triton X-100 (VC). The analytical parameters and procedure of electrode activation were optimized. The calibration graphs obtained for vitamins C, B(1) and B(2) are linear, respectively, for concentration range 0.05-12, 0.01-0.1 and 0.05-3 mg L(-1). The detection limits were calculated and equaled 0.02, 0.003 and 0.009 mg L(-1), while repeatability of the peak current was 2%, 1% and 3%, respectively. These results are comparable with results obtained for polarographic determination of the same vitamins using mercury electrodes. Finally, the AgLAF-AgSAE was applied to the determination of vitamins in pharmaceutical samples and fruit juices with satisfactory results.
authors.affiliation: Faculty of Materials Science and Ceramics, AGH University of Science and Technology, 30-059 Kraków, al. Mickiewicza 30, Poland. bas@agh.edu.pl
authors.forename: Bogusław
authors.initials: B
authors.lastname: Baś
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Jakubowska
authors.forename: Łukasz
authors.initials: Ł
authors.lastname: Górski
mesh_terms: D000497:Alloys / Q000737:chemistry*; D001205:Ascorbic Acid / Q000032:analysis / Q000737:chemistry; D001628:Beverages / Q000032:analysis; D002021:Buffers; D002138:Calibration; D004563:Electrochemistry / Q000295:instrumentation*; D004566:Electrodes; D004573:Electrolytes / Q000737:chemistry; D017743:Equipment Reuse; D008628:Mercury / Q000737:chemistry*; D004364:Pharmaceutical Preparations / Q000737:chemistry; D012256:Riboflavin / Q000032:analysis / Q000737:chemistry; D012834:Silver / Q000737:chemistry*; D013831:Thiamine / Q000032:analysis / Q000737:chemistry; D014815:Vitamins / Q000032:analysis* / Q000737:chemistry
chemical_list: D000497:Alloys; D002021:Buffers; D004573:Electrolytes; D004364:Pharmaceutical Preparations; D014815:Vitamins; D012834:Silver; D008628:Mercury; D001205:Ascorbic Acid; D012256:Riboflavin; D013831:Thiamine