title: MiR-34a inhibits lymphatic metastasis potential of mouse hepatoma cells.
abstract: MicroRNAs are small non-coding RNAs that regulate the expression of other genes in a post-transcriptional manner. MiR-34a can induce apoptosis, cell cycle arrest, and senescence. However, its role in tumor progress remains to be fully elucidated. In the present study, the role of miR-34a in lymphatic metastasis was investigated using mouse hepatocarcinoma cell lines Hca-F and Hepa1-6. MicroRNA profiling and Hairpin-RT-PCR analysis showed that the expression level of miR-34a was higher in Hepa1-6 cells (of no metastatic ability) than that in Hca-F cells (of high metastatic ability). Ectopic expression of miR-34a can inhibit cell growth and cell invasion in Hepa1-6 and Hca-F cells. Moreover, miR-34a triggers G1 arrest and down-regulates CyclinD1 and CDK6 in Hepa1-6 cells. Furthermore, we proved that miR-34a decreased adhesion of Hca-F cells to regional lymph node in vitro, reduced lymph nodes-metastasized burden, and inhibited tumor lymph node metastases in vivo. All these results suggest that miR-34a plays multiple tumor suppressive roles in murine hepatocarcinoma, not only inhibiting cell growth by cell cycle arrest, but also repressing metastasis, and may serve as a novel therapeutic target for hepatocarcinoma.
authors.affiliation: Department of Biochemistry, Institute of Glycobiology, Dalian Medical University, 9 South Lvshun Road Western Section, Dalian, 116044 Liaoning, China.
authors.forename: Yanjie
authors.initials: Y
authors.lastname: Guo
authors.forename: Sheng
authors.initials: S
authors.lastname: Li
authors.forename: Jianhua
authors.initials: J
authors.lastname: Qu
authors.forename: Shujing
authors.initials: S
authors.lastname: Wang
authors.forename: Yibing
authors.initials: Y
authors.lastname: Dang
authors.forename: Jianhui
authors.initials: J
authors.lastname: Fan
authors.forename: Shengjin
authors.initials: S
authors.lastname: Yu
authors.forename: Jianing
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000473:pathology*; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D016193:G1 Phase; D008198:Lymph Nodes / Q000473:pathology; D008207:Lymphatic Metastasis / Q000235:genetics*; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D009368:Neoplasm Transplantation; D014158:Transcription, Genetic; D047368:Tumor Burden
chemical_list: C551848:MIRN34a microRNA, mouse; D035683:MicroRNAs