title: Autoimmune melanocyte destruction is required for robust CD8+ memory T cell responses to mouse melanoma.
abstract: A link between autoimmunity and improved antitumor immunity has long been recognized, although the exact mechanistic relationship between these two phenomena remains unclear. In the present study we have found that vitiligo, the autoimmune destruction of melanocytes, generates self antigen required for mounting persistent and protective memory CD8+ T cell responses to melanoma. Vitiligo developed in approximately 60% of mice that were depleted of regulatory CD4+ T cells and then subjected to surgical excision of large established B16 melanomas. Mice with vitiligo generated 10-fold larger populations of CD8+ memory T cells specific for shared melanoma/melanocyte antigens. CD8+ T cells in mice with vitiligo acquired phenotypic and functional characteristics of effector memory, suggesting that they were supported by ongoing antigen stimulation. Such responses were not generated in melanocyte-deficient mice, indicating a requirement for melanocyte destruction in maintaining CD8+ T cell immunity to melanoma. Vitiligo-associated memory CD8+ T cells provided durable tumor protection, were capable of mounting a rapid recall response to melanoma, and did not demonstrate phenotypic or functional signs of exhaustion even after many months of exposure to antigen. This work establishes melanocyte destruction as a key determinant of lasting melanoma-reactive immune responses, thus illustrating that immune-mediated destruction of normal tissues can perpetuate adaptive immune responses to cancer.
authors.affiliation: Department of Microbiology and Immunology, Dartmouth Medical School, Lebanon, New Hampshire, USA.
authors.forename: Katelyn T
authors.initials: KT
authors.lastname: Byrne
authors.forename: Anik L
authors.initials: AL
authors.lastname: Côté
authors.forename: Peisheng
authors.initials: P
authors.lastname: Zhang
authors.forename: Shannon M
authors.initials: SM
authors.lastname: Steinberg
authors.forename: Yanxia
authors.initials: Y
authors.lastname: Guo
authors.forename: Rameeza
authors.initials: R
authors.lastname: Allie
authors.forename: Weijun
authors.initials: W
authors.lastname: Zhang
authors.forename: Marc S
authors.initials: MS
authors.lastname: Ernstoff
authors.forename: Edward J
authors.initials: EJ
authors.lastname: Usherwood
authors.forename: Mary Jo
authors.initials: MJ
authors.lastname: Turk
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology*; D016207:Cytokines / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006706:Homeostasis; D007156:Immunologic Memory; D007371:Interferon-gamma / Q000378:metabolism; D008544:Melanocytes / Q000166:cytology*; D008545:Melanoma / Q000378:metabolism*; D008546:Melanoma, Experimental; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D012878:Skin Neoplasms / Q000378:metabolism*; D014820:Vitiligo / Q000378:metabolism
chemical_list: D016207:Cytokines; D007371:Interferon-gamma