title: Genetic analysis of basophil function in vivo.
abstract: Contributions by basophils to allergic and helminth immunity remain incompletely defined. Using sensitive interleukin 4 (Il4) reporter alleles, we demonstrate here that basophil IL-4 production occurs by a CD4(+) T cell-dependent process restricted to the peripheral tissues affected. We genetically marked and achieved specific deletion of basophils and found that basophils did not mediate T helper type 2 (T(H)2) priming in vivo. Two-photon imaging confirmed that basophils did not interact with antigen-specific T cells in lymph nodes but engaged in prolonged serial interactions with T cells in lung tissues. Although targeted deletion of IL-4 and IL-13 in either CD4(+) T cells or basophils had a minimal effect on worm clearance, deletion from both lineages demonstrated a nonredundant role for basophil cytokines in primary helminth immunity.
authors.affiliation: Howard Hughes Medical Institute, University of California San Francisco, San Francisco, California, USA.
authors.forename: Brandon M
authors.initials: BM
authors.lastname: Sullivan
authors.forename: Hong-Erh
authors.initials: HE
authors.lastname: Liang
authors.forename: Jennifer K
authors.initials: JK
authors.lastname: Bando
authors.forename: Davina
authors.initials: D
authors.lastname: Wu
authors.forename: Laurence E
authors.initials: LE
authors.lastname: Cheng
authors.forename: James K
authors.initials: JK
authors.lastname: McKerrow
authors.forename: Christopher D C
authors.initials: CD
authors.lastname: Allen
authors.forename: Richard M
authors.initials: RM
authors.lastname: Locksley
mesh_terms: D000818:Animals; D001491:Basophils / Q000276:immunology* / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006374:Helminthiasis, Animal / Q000276:immunology / Q000378:metabolism / Q000469:parasitology; D006790:Host-Parasite Interactions / Q000276:immunology; D006801:Humans; D018793:Interleukin-13 / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D015847:Interleukin-4 / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D008099:Liver / Q000276:immunology / Q000378:metabolism / Q000469:parasitology; D008168:Lung / Q000276:immunology* / Q000378:metabolism / Q000469:parasitology; D008174:Lung Diseases, Parasitic / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D036641:Microscopy, Fluorescence, Multiphoton; D009559:Nippostrongylus / Q000276:immunology / Q000502:physiology; D012550:Schistosoma mansoni / Q000276:immunology / Q000502:physiology; D017206:Strongylida Infections / Q000276:immunology* / Q000378:metabolism / Q000469:parasitology
chemical_list: D018793:Interleukin-13; D049452:Green Fluorescent Proteins; D015847:Interleukin-4