title: Application of trichloroacetic acid-acetone precipitation method for protein extraction in bone tissue.
abstract: OBJECTIVE
  To explore the feasibility and efficiency of extracting protein from bone tissue using trichloroacetic acid (TCA)-acetone precipitation method.
  
  
  METHODS
  Hydrochloric acid (HCL) decalcification method and TCA-acetone precipitation method were separately used for bone protein extraction. The efficiencies of these two methods were compared.
  
  
  RESULTS
  TCA-acetone precipitation method had significantly higher extraction efficiency. Compared with HCL decalcification method, it had less pollution from bone marrow hematopoietic tissue. Protein band distribution was similar between these two methods.
  
  
  CONCLUSIONS
  TCA-acetone precipitation method is useful for bone proteomics research.
authors.affiliation: Department of Orthopedics, the First People's Hospital of Xiaoshan District, Hangzhou 311200, China. guke120@hotmail.com
authors.forename: Cui
authors.initials: C
authors.lastname: Yong-feng
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhen
authors.forename: Zhu
authors.initials: Z
authors.lastname: Bao-hua
authors.forename: Xiao
authors.initials: X
authors.lastname: Lu-wei
authors.forename: Tong
authors.initials: T
authors.lastname: Pei-jian
mesh_terms: D000096:Acetone; D000818:Animals; D001842:Bone and Bones / Q000737:chemistry*; D011232:Chemical Precipitation; D008297:Male; D011506:Proteins / Q000302:isolation & purification*; D040901:Proteomics / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar; D014238:Trichloroacetic Acid
chemical_list: D011506:Proteins; D000096:Acetone; D014238:Trichloroacetic Acid