title: Impact of heparan sulfate chains and sulfur-mediated bonds on the mechanical properties of bovine lens capsule.
abstract: We assessed the importance of glycosaminoglycans and sulfur-mediated bonds for the mechanical properties of lens capsules by comparing the stress-strain responses from control and treated pairs of bovine source. No significant change in mechanical properties was observed upon reduction of disulfide bonds. However, removal of glycosaminoglycan chains resulted in a significantly stiffer lens capsule, whereas high concentrations of reducing agent, which is expected to reduce the recently reported sulfilimine bond of collagen IV, resulted in a significantly less stiff lens capsule. A comparison of the diffraction patterns of the control and strongly reduced lens capsules indicated structural rearrangements on a nanometer scale.
authors.affiliation: CSIRO Materials Science and Engineering, Clayton, Australia. leanne.dyksterhuis@monash.edu
authors.forename: L B
authors.initials: LB
authors.lastname: Dyksterhuis
authors.forename: L D
authors.initials: LD
authors.lastname: Dyksterhuis
authors.forename: J F
authors.initials: JF
authors.lastname: White
authors.forename: M
authors.initials: M
authors.lastname: Hickey
authors.forename: N
authors.initials: N
authors.lastname: Kirby
authors.forename: S
authors.initials: S
authors.lastname: Mudie
authors.forename: A
authors.initials: A
authors.lastname: Hawley
authors.forename: A
authors.initials: A
authors.lastname: Vashi
authors.forename: J
authors.initials: J
authors.lastname: Nigro
authors.forename: J A
authors.initials: JA
authors.lastname: Werkmeister
authors.forename: J A M
authors.initials: JA
authors.lastname: Ramshaw
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D002417:Cattle; D011508:Chondroitin Sulfate Proteoglycans / Q000737:chemistry; D004586:Electrophoresis; D006497:Heparan Sulfate / Q000737:chemistry*; D006820:Hyaluronic Acid / Q000737:chemistry; D007903:Lens Capsule, Crystalline / Q000737:chemistry*; D010084:Oxidation-Reduction; D015203:Reproducibility of Results; D013314:Stress, Mechanical; D013455:Sulfur / Q000737:chemistry*
chemical_list: D011508:Chondroitin Sulfate Proteoglycans; D006497:Heparan Sulfate; D006820:Hyaluronic Acid; D013455:Sulfur