title: Chemically induced self-assembly of enzyme nanorings.
abstract: Continued exploration into the field of chemically induced dimerization (CID) has revealed a number of applications for its use in a broader context as a method of structural assembly (1-4). In particular, the use of CID technology to generate self-assembled (and selectively disassembled) protein toroids serves as a key advancement toward developing stable and controllable protein-based platforms. Such structures have broad application to the development of novel therapeutics, lab-on-a-chip technologies, and multi-enzyme assemblies (5, 6). This chapter describes a method of developing an enzymatically active protein nanostructure incorporating both a CID-based assembly region containing dihydrofolate reductase (DHFR) and an enzymatic region consisting of histidine triad nucleotide binding protein 1 (Hint1). Details of both the production and the characterization of this structure are provided.
authors.affiliation: Department of Medicinal Chemistry, University of Minnesota, Minneapolis, MN 55455, USA.
authors.forename: Brian R
authors.initials: BR
authors.lastname: White
authors.forename: Qing
authors.initials: Q
authors.lastname: Li
authors.forename: Carston R
authors.initials: CR
authors.lastname: Wagner
mesh_terms: D001665:Binding Sites / Q000187:drug effects; D002850:Chromatography, Gel; D003001:Cloning, Molecular; D004926:Escherichia coli; D015870:Gene Expression; D006639:Histidine / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008727:Methotrexate / Q000737:chemistry* / Q000494:pharmacology; D008958:Models, Molecular; D058726:Nanoconjugates / Q000032:analysis / Q000737:chemistry; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D055503:Protein Multimerization / Q000187:drug effects*; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013762:Tetrahydrofolate Dehydrogenase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: C063306:HINT1 protein, human; D058726:Nanoconjugates; D009419:Nerve Tissue Proteins; D011993:Recombinant Fusion Proteins; D006639:Histidine; D013762:Tetrahydrofolate Dehydrogenase; D008727:Methotrexate