title: Study of the interaction between ofloxacin and human serum albumin by spectroscopic methods.
abstract: The binding of ofloxacin (OFLX) to human serum albumin (HSA) was investigated by fluorescence and circular dichroism (CD) techniques. The binding parameters have been evaluated by a fluorescence quenching method. Competitive binding measurements were performed in the presence of warfarin and ibuprofen and suggest binding to the warfarin site I of HSA. The distance r between donor (HSA) and acceptor (OFLX) was estimated according to the Forster's theory of non-radiatiative energy transfer. CD spectra revealed that the binding of OFLX to HSA induced conformational changes in HSA. Molecular docking was performed and shows that for the lowest energy complex OFLX is located in site I of HSA, which correlate to the competitive binding experiments.
authors.affiliation: Department of Physical Chemistry, University of Bucharest, Bd. Regina Elisabeta 4-12, Bucharest, Romania.
authors.forename: Aurica
authors.initials: A
authors.lastname: Varlan
authors.forename: Sorana
authors.initials: S
authors.lastname: Ionescu
authors.forename: Mihaela
authors.initials: M
authors.lastname: Hillebrand
mesh_terms: D002942:Circular Dichroism; D006801:Humans; D015242:Ofloxacin / Q000737:chemistry*; D012709:Serum Albumin / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D012709:Serum Albumin; D015242:Ofloxacin