title: Expression of transforming growth factor-beta 1 and connective tissue growth factor in women with pelvic organ prolapse.
abstract: OBJECTIVE
  To investigate the presence of transforming growth factor-beta 1 (TGF-ß1) and connective tissue growth factor (CTGF) in women with pelvic organ prolapse (POP).
  
  
  METHODS
  This study was conducted from May to December 2009. Fifty patients with POP that underwent vaginal hysterectomy in the Department of Gynecology, Renmin Hospital of Wuhan University, Hubei, Wuhan, China were enrolled in this study. They were divided into: Group 1 (n=10); Group 2 (n=10); and Group 3 (n=10) according to Pelvic Organ Prolapse Quantitation (POP-Q). Meanwhile, 20 cases treated by vaginal hysterectomy for other benign gynecological diseases were selected as the control group. Immunohistochemical staining and Western blot were performed to detect the expression of TGF-ß1 and CTGF.
  
  
  RESULTS
  Immunohistochemical staining of TGF-ß1 and CTGF were mainly expressed in the cytoplasm of fibroblast cells. The expression of TGF-ß1 and CTGF protein was significantly negatively correlated with POP-Q stage. There were significantly positive correlations between the expression of TGF-ß1 and CTGF protein. The expression of TGF-ß1 protein among the 3 POP groups were all significantly lower than that of the control group, while there was no significant differences in the expression of TGF-ß1 protein among the POP groups, excluding the comparison between Groups 1 and 3. The expression of CTGF protein in the 3 POP groups were all significantly lower than that of the control group, and significant differences were also detected among the 3 POP groups.
  
  
  CONCLUSIONS
  In this study, we found that the TGF-ß1 and CTGF protein expression may be associated with POP, especially in POP-Q stages.
authors.affiliation: Department of Gynecology and Obstetrics, Renmin Hospital of Wuhan University, Jiefang Road 238, Wuhan, Hubei 430060, China.
authors.forename: Xiao-Ying
authors.initials: XY
authors.lastname: Qi
authors.forename: Li
authors.initials: L
authors.lastname: Hong
authors.forename: Feng-Qin
authors.initials: FQ
authors.lastname: Guo
authors.forename: Qiong
authors.initials: Q
authors.lastname: Fu
authors.forename: Lu
authors.initials: L
authors.lastname: Chen
authors.forename: Bing-Shu
authors.initials: BS
authors.lastname: Li
mesh_terms: D000328:Adult; D015153:Blotting, Western; D016022:Case-Control Studies; D055513:Connective Tissue Growth Factor / Q000378:metabolism*; D005260:Female; D006801:Humans; D007045:Hysterectomy, Vaginal; D007150:Immunohistochemistry; D008875:Middle Aged; D056887:Pelvic Organ Prolapse / Q000378:metabolism* / Q000601:surgery; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*
chemical_list: D053773:Transforming Growth Factor beta1; D055513:Connective Tissue Growth Factor