title: Sensitive on-chip detection of a protein biomarker in human serum and plasma over an extended dynamic range using silicon photonic microring resonators and sub-micron beads.
abstract: We demonstrate a three-step assay on a silicon photonic microring resonator-based detection platform that enables the quantitation of the cardiac biomarker C-reactive protein (CRP) over a dynamic range spanning six orders of magnitude. Using antibody-modified microrings, we sequentially monitor primary CRP binding, secondary recognition of bound CRP by a biotinylated antibody, and tertiary signal amplification using streptavidin-functionalized beads. This detection methodology is applied to CRP quantitation in human serum and plasma samples.
authors.affiliation: Department of Chemistry, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, Illinois 61801, USA.
authors.forename: Matthew S
authors.initials: MS
authors.lastname: Luchansky
authors.forename: Adam L
authors.initials: AL
authors.lastname: Washburn
authors.forename: Melinda S
authors.initials: MS
authors.lastname: McClellan
authors.forename: Ryan C
authors.initials: RC
authors.lastname: Bailey
mesh_terms: D015415:Biomarkers / Q000032:analysis*; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D002097:C-Reactive Protein / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods; D010316:Particle Size; D010949:Plasma / Q000737:chemistry*; D044967:Serum / Q000737:chemistry*; D012825:Silicon / Q000737:chemistry*
chemical_list: D015415:Biomarkers; D002097:C-Reactive Protein; D012825:Silicon