title: Increased number of Arginine-based salt bridges contributes to the thermotolerance of thermotolerant acetic acid bacteria, Acetobacter tropicalis SKU1100.
abstract: Thermotolerant acetic acid bacteria (AAB), Acetobacter tropicalis SKU1100, can grow above 40°C. To investigate the basis of its thermotolerance, we compared the genome of A. tropicalis SKU1100 with that of mesophilic AAB strain Acetobacter pasteurianus IFO3283-01. The comparative genomic study showed that amino acid substitutions from large to small residue and Lys to Arg occur in many orthologous genes. Furthermore, comparative modeling study was carried out with the orthologous proteins between SKU1100 and IFO3283-01 strains, indicating that the number of Arg-based salt bridges increased in protein models. Since it has been reported that Arg-based salt bridges are important factor for thermo-stability of protein structure, our results strongly suggest that the increased number of Arg-based salt bridges may contributes to the thermotolerance of A. tropicalis SKU1100 (the thermo-stability of proteins in A. tropicalis SKU1100).
authors.affiliation: Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Japan.
authors.forename: Minenosuke
authors.initials: M
authors.lastname: Matsutani
authors.forename: Hideki
authors.initials: H
authors.lastname: Hirakawa
authors.forename: Mitsuteru
authors.initials: M
authors.lastname: Nishikura
authors.forename: Wichai
authors.initials: W
authors.lastname: Soemphol
authors.forename: Ibnaof Ali Ibnaof
authors.initials: IA
authors.lastname: Ali
authors.forename: Toshiharu
authors.initials: T
authors.lastname: Yakushi
authors.forename: Kazunobu
authors.initials: K
authors.lastname: Matsushita
mesh_terms: D000091:Acetobacter / Q000235:genetics / Q000502:physiology*; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D001120:Arginine / Q000737:chemistry* / Q000235:genetics; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D016680:Genome, Bacterial; D006358:Hot Temperature; D008239:Lysine / Q000737:chemistry / Q000235:genetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D055550:Protein Stability
chemical_list: D001426:Bacterial Proteins; D001120:Arginine; D008239:Lysine