title: Improved identification of yeast species directly from positive blood culture media by combining Sepsityper specimen processing and Microflex analysis with the matrix-assisted laser desorption ionization Biotyper system.
abstract: Current methods for identification of yeast from blood cultures may take several days after these microorganisms have been observed by Gram stain smears from positive blood cultures. We explored the use of a matrix-assisted laser desorption ionization (MALDI) Biotyper system in combination with Sepsityper specimen processing and Microflex analysis for improved detection and identification of yeast species directly from positive blood culture specimens demonstrating yeast-like organisms by Gram stain. The limit of detection of yeast species in blood culture medium was determined to be 5.9 × 10(5) CFU, with intra- and interstrain coefficients of variation of 1.8 to 3.6% and 2.9%, respectively. A total of 42 yeast-containing positive blood culture specimens were processed, and the identification results were compared to those obtained by routinely used phenotypic methods. Specimens with discrepant results between the Biotyper and phenotypic methods were identified on the basis of internal transcribed spacer region sequencing. The MALDI Biotyper system correctly identified the 42 specimens to species level, including 28 (66.7%) Candida albicans, 8 (19.0%) Candida parapsilosis, and 5 (11.9%) Candida tropicalis isolates and 1 (2.4%) Cryptococcus neoformans isolate. The entire procedure, from specimen extraction to final result reporting, can be completed within 1 h. Our data indicated that the Sepsityper specimen processing and Microflex analysis by the MALDI Biotyper system provide a rapid and reliable tool for yeast species identification directly from positive blood culture media.
authors.affiliation: Department of Pathology and Medicine, Vanderbilt University Medical Center, Nashville, Tennessee 37232-5310, USA.
authors.forename: Yingjun
authors.initials: Y
authors.lastname: Yan
authors.forename: Ying
authors.initials: Y
authors.lastname: He
authors.forename: Thomas
authors.initials: T
authors.lastname: Maier
authors.forename: Criziel
authors.initials: C
authors.lastname: Quinn
authors.forename: Gongyi
authors.initials: G
authors.lastname: Shi
authors.forename: Haijing
authors.initials: H
authors.lastname: Li
authors.forename: Charles W
authors.initials: CW
authors.lastname: Stratton
authors.forename: Markus
authors.initials: M
authors.lastname: Kostrzewa
authors.forename: Yi-Wei
authors.initials: YW
authors.lastname: Tang
mesh_terms: D001769:Blood / Q000382:microbiology*; D019411:Clinical Laboratory Techniques / Q000379:methods*; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009172:Mycology / Q000379:methods*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D015003:Yeasts / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification*
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer