title: A novel antimicrobial peptide from skin secretions of the earthworm, Pheretima guillelmi (Michaelsen).
abstract: A novel lumbricin-like antimicrobial peptide named lumbricin-PG was isolated from skin secretions of the earthworm, Pheretima guillelmi (Michaelsen), using a procedure of one step Sephadex G-50 gel filtration and one step C(8) reverse-phase high-performance liquid chromatography (RP-HPLC). Its amino acid sequence was determined as FSRYARMRDSRPWSDRKNNYSGPQFTYPPEKAPPEKLIKWNN EGSPIFEMPAEGGHIEP by Edman degradation combined with cDNA cloning and mass spectrometry analysis. The cDNA encoding lumbricin-PG was cloned by cDNA library screening. The predicted protein from the cDNA sequence was composed of 73 amino acid residues including a mature lumbricin-PG and predicted signal peptide. It showed similarity with lumbricin antimicrobial peptide from the earthworm, Lumbricus rubellus by BLAST search. Purified lumbricin-PG exerted potential antimicrobial activities against bacteria and fungi; it showed weak hemolysis activity against human and rabbit red cells.
authors.affiliation: Oncology Department, The First Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan, China.
authors.forename: Wenliang
authors.initials: W
authors.lastname: Li
authors.forename: Sisi
authors.initials: S
authors.lastname: Li
authors.forename: Jian
authors.initials: J
authors.lastname: Zhong
authors.forename: Zhu
authors.initials: Z
authors.lastname: Zhu
authors.forename: Jingze
authors.initials: J
authors.lastname: Liu
authors.forename: Wenhong
authors.initials: W
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D012634:Bodily Secretions / Q000737:chemistry* / Q000378:metabolism; D002176:Candida albicans / Q000187:drug effects; D056148:Chromatography, Reverse-Phase; D003001:Cloning, Molecular; D004912:Erythrocytes / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D015723:Gene Library; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D013058:Mass Spectrometry; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009835:Oligochaeta / Q000235:genetics / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D011817:Rabbits; D012867:Skin / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D010455:Peptides; C115676:lumbricin I