title: Glutaraldehyde-activated chitosan matrix for immobilization of a novel cysteine protease, procerain B.
abstract: Proteases have several applications in the food industry. We report the immobilization of procerain B, a novel cysteine protease, on glutaraldehyde-activated chitosan beads through covalent attachment. Glutaraldehyde not only serves as a cross-linking agent but also links the procerain B on the surface of bead through primary amine group (either lysine side chain or N-terminal) by Schiff base linkage. Immobilized procerain B was characterized for optimum functional range and stability with respect to pH and temperature. The chitosan-immobilized procerain B has broad pH and thermal optima. The effects of substrate concentration and reusability of immobilized beads were also studied. It showed nearly 50% activity until the 10th use.
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Guwahati, Assam, India 781039.
authors.forename: Abhay Narayan
authors.initials: AN
authors.lastname: Singh
authors.forename: Sushant
authors.initials: S
authors.lastname: Singh
authors.forename: Neeraj
authors.initials: N
authors.lastname: Suthar
authors.forename: Vikash Kumar
authors.initials: VK
authors.lastname: Dubey
mesh_terms: D031107:Calotropis / Q000737:chemistry / Q000201:enzymology*; D048271:Chitosan / Q000737:chemistry; D003546:Cysteine Endopeptidases / Q000737:chemistry*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry; D005976:Glutaral / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010940:Plant Proteins / Q000737:chemistry*; D013696:Temperature
chemical_list: D004800:Enzymes, Immobilized; D010940:Plant Proteins; D048271:Chitosan; D003546:Cysteine Endopeptidases; C474045:procerain; D005976:Glutaral