title: Algoriphagus aquaeductus sp. nov., isolated from a freshwater pipe.
abstract: A Gram-staining-negative, aerobic to microaerophilic, rod-shaped, red-coloured bacterium, strain T4(T), was isolated from a freshwater pipe on Tenerife island. A polyphasic taxonomic study was performed in order to characterize the strain in detail. The isolate is surrounded by a slime capsule, occurs singly, in the form of short chains, or in aggregates, and exhibits catalase and oxidase activities. Growth was observed at 15-42 °C. Optimum growth occurred at pH 8 with mono- and disaccharides, followed by polysaccharides and deoxysaccharides, but the bacterium utilized only a restricted spectrum of alcohols, alditols, amides, amines, carboxylic acids and amino acids. Strain T4(T) tolerated concentrations of 0-4% (w/v) NaCl and contained MK-7 as predominant isoprenoid quinone as well as carotenoids, but lacked pigments of the flexirubin type. The predominant fatty acids were iso-C(15:0) (32.2%), summed feature 3 (C(16:1)ω6c and/or C(16:1)ω7c; 22.5%), and iso-C(17:0) 3-OH (7.9%). Major polar lipids were phosphatidylethanolamine, phospholipids, aminophospholipids and other lipids of unknown character. The DNA G+C content was approximately 41.8 mol%. The sequence of the 16S-rRNA gene assigned strain T4(T) to the CFB group, forming a coherent cluster with species of the genus Algoriphagus with the highest similarity of 98.8% to Algoriphagus aquatilis A8-7(T). DNA-DNA hybridization revealed 37.5% relatedness to strain A8-7(T). Based on morphological, physiological and molecular properties as well as on phylogenetic distinctiveness, strain T4(T) should be placed into the genus Algoriphagus as a novel species, for which the name Algoriphagus aquaeductus sp. nov. (type strain T4(T) =DSM 19759(T) =LMG 24398(T) =NCIMB 14399(T)) is proposed.
authors.affiliation: Zentrum für Umweltforschung und nachhaltige Technologien, Fachbereich Biologie/Chemie, Abteilung Marine Mikrobiologie, Universität Bremen, Leobenerstraße, D-28359 Bremen, Germany.
authors.forename: Jan Erik
authors.initials: JE
authors.lastname: Rau
authors.affiliation: Zentrum für Umweltforschung und nachhaltige Technologien, Fachbereich Biologie/Chemie, Abteilung Marine Mikrobiologie, Universität Bremen, Leobenerstraße, D-28359 Bremen, Germany.
authors.forename: Karl-Heinz
authors.initials: KH
authors.lastname: Blotevogel
authors.affiliation: Zentrum für Umweltforschung und nachhaltige Technologien, Fachbereich Biologie/Chemie, Abteilung Marine Mikrobiologie, Universität Bremen, Leobenerstraße, D-28359 Bremen, Germany.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Fischer
mesh_terms: D000332:Aerobiosis; D016667:Bacterial Capsules / Q000378:metabolism; D015373:Bacterial Typing Techniques; D041963:Bacteroidetes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D001482:Base Composition; D016227:Benzoquinones / Q000032:analysis; D002244:Carbon / Q000378:metabolism; D002338:Carotenoids / Q000032:analysis; D002374:Catalase / Q000378:metabolism; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D060766:Drinking Water / Q000382:microbiology*; D004734:Energy Metabolism; D005227:Fatty Acids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010088:Oxidoreductases / Q000378:metabolism; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D010860:Pigments, Biological / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature
chemical_list: D016227:Benzoquinones; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D060766:Drinking Water; D005227:Fatty Acids; D010743:Phospholipids; D010860:Pigments, Biological; D012336:RNA, Ribosomal, 16S; D002338:Carotenoids; C004532:quinone; D012965:Sodium Chloride; D002244:Carbon; D010088:Oxidoreductases; D002374:Catalase