title: [Effect and mechanism of Aconitum vaginatum on the proliferation, invasion and metastasis in human A549 lung carcinoma cells].
abstract: OBJECTIVE
  To study the effects and mechanism of Aconitum vaginatum on proliferation, invasion and metastasis of human lung adenocarcinoma A549 cell lines.
  
  
  METHODS
  A549 cells were treated with Aconitum vaginatum at different concentrations, and divided into 4 groups: Aconitum vaginatum 0.01, 0.1, 1 mg/mL groups and control group, respectively. MTT assay was used to evaluate cell proliferation; migration and invasion (with matrigel) assay was conducted in the transwell chamber; gelatin zymography was performed to evaluated the impacts of Aconitum vaginatum on matrix metalloproteinase (MMP)-2, MMP-9.
  
  
  RESULTS
  Aconitum vaginatum exhibited a concentration-dependent inhibitory on proliferation and invasion of A549 cells. Aconitum vaginatum was also found to decrease the activity of MMP-2, MMP-9 in a concentration-dependent manner (P < 0.05).
  
  
  CONCLUSIONS
  Aconitum vaginatum can inhibit the proliferation, invasion and metastasis of human adenocarcinoma A549 cell lines, the mechanism maybe deceasing MMP-2, MMP-9 activity.
authors.affiliation: Department of Pharmacy, The People's Hospital of Yunyang, Shiyan 442000, China. xxf_120@yahoo.cn
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Xu
authors.forename: Yin-Hua
authors.initials: YH
authors.lastname: Chen
authors.forename: Ju
authors.initials: J
authors.lastname: Wang
authors.forename: Jia-Jun
authors.initials: JJ
authors.lastname: Liao
authors.forename: Jin-Hu
authors.initials: JH
authors.lastname: Wu
mesh_terms: D031841:Aconitum / Q000737:chemistry*; D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology*; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9