title: Intracellular Ca(2+) levels in rat ventricle cells exposed to extremely low frequency magnetic field.
abstract: OBJECTIVE
  Electromagnetic fields can affect intracellular Ca(2+) levels. The aim of this study was to determine the changes intracellular Ca(2+) concentration in cardiac ventricle cells of rats exposed to 0.25 mT (2.5 Gauss) magnetic field.
  
  
  METHODS
  Forty-five male rats were introduced to this study. The rats were divided into three groups: control, sham, and experiment. The experimental group was exposed to 0.25 mT extremely low frequency (ELF) magnetic field for 14 days, 3 h/day. The sham group was treated like the experimental group, except for elf-magnetic field exposure. The control group was not subjected to anything and differed from the experimental group and sham group. In the end of experiment, rats were sacrificed, cardiac tissue was removed, and these were fixed in 10% neutral formalin. Then, ventricular cells were stained by Alizarin red staining method.
  
  
  RESULTS
  In the light microscopic examinations of control groups, in myofibril structures between groups, changes were not observed. In myofibril regions of the experimental group compared to other groups, increased heterogen Ca(2+) accumulations were found.
  
  
  CONCLUSIONS
  ELF magnetic fields are used in daily life. The results of this study show that intracellular Ca(2+) accumulation in cardiac ventricles can increase in rats exposed to ELF magnetic field.
authors.affiliation: Department of Biophysics, Medicine Faculty of Harran University, Sanliurfa, Turkey. csert@harran.edu.tr
authors.forename: Cemil
authors.initials: C
authors.lastname: Sert
authors.forename: Sevda
authors.initials: S
authors.lastname: Söker
authors.forename: Mustafa
authors.initials: M
authors.lastname: Deniz
authors.forename: Yusuf
authors.initials: Y
authors.lastname: Nergiz
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism / Q000528:radiation effects*; D002413:Cations, Divalent / Q000378:metabolism / Q000528:radiation effects; D004574:Electromagnetic Fields; D006352:Heart Ventricles / Q000166:cytology* / Q000378:metabolism / Q000528:radiation effects*; D007425:Intracellular Membranes / Q000378:metabolism / Q000528:radiation effects*; D017136:Ion Transport / Q000528:radiation effects; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
chemical_list: D002413:Cations, Divalent; D002118:Calcium