title: Differential roles of calcitonin family peptides in the dendrite formation and spinogenesis of the cerebral cortex in vitro.
abstract: We examined roles of calcitonin family peptides in the initial stages of dendrite formation and the maturation of dendritic spines in the rat cerebral cortex in vitro. Embryonic day 18 cortical neurons were dissociated and cultured for 2-3days in the presence of calcitonin gene-related peptide (CGRP), calcitonin, amylin or adrenomedullin. The treatment of cortical neurons with CGRP promoted the formation of primary dendrites of non-GABAergic neurons. In contrast, the treatment with amylin and adrenomedullin for 3days inhibited the dendritic elongation of non-GABAergic neurons. Calcitonin had no effect on the initial dendrite formation. Next, we examined roles of the peptides in the spine formation. Embryonic day 16 cortical neurons were cultured for 14days and then treated acutely with CGRP, amylin or adrenomedullin for 24h. The density of filopodia, puncta/stubby spines and spines were increased by the CGRP treatment, whereas decreased by amylin. Therefore, CGRP and amylin showed opposite effects on the formation of dendritic filopodia, puncta and spines. Adrenomedullin had no effects on the spine formation. In conclusion, the present study showed that calcitonin family peptides have differential effects both in the dendrite formation during the initial stages and the spine formation of cortical neurons in vitro.
authors.affiliation: University of Tsukuba, Graduate School of Comprehensive Human Sciences, Doctoral Program in Kansei, Behavioral and Brain Sciences, Tennodai, Japan.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Harigai
authors.forename: Masaki
authors.initials: M
authors.lastname: Natsume
authors.forename: Fei
authors.initials: F
authors.lastname: Li
authors.forename: Akiko
authors.initials: A
authors.lastname: Ohtani
authors.forename: Kouji
authors.initials: K
authors.lastname: Senzaki
authors.forename: Takashi
authors.initials: T
authors.lastname: Shiga
mesh_terms: D053607:Adrenomedullin / Q000494:pharmacology; D000818:Animals; D002116:Calcitonin / Q000494:pharmacology*; D015740:Calcitonin Gene-Related Peptide / Q000494:pharmacology; D058286:Calcitonin Receptor-Like Protein / Q000378:metabolism; D002540:Cerebral Cortex / Q000166:cytology* / Q000196:embryology; D003712:Dendrites / Q000187:drug effects* / Q000502:physiology* / Q000648:ultrastructure; D049229:Dendritic Spines / Q000187:drug effects* / Q000502:physiology*; D005260:Female; D058228:Islet Amyloid Polypeptide / Q000494:pharmacology; D009474:Neurons / Q000502:physiology / Q000648:ultrastructure; D010455:Peptides / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D058261:Receptor Activity-Modifying Proteins / Q000378:metabolism
chemical_list: D058286:Calcitonin Receptor-Like Protein; C545580:Calcrl protein, rat; D058228:Islet Amyloid Polypeptide; D010455:Peptides; D058261:Receptor Activity-Modifying Proteins; D053607:Adrenomedullin; D002116:Calcitonin; D015740:Calcitonin Gene-Related Peptide