title: Comparison of three strip-type tests and two laboratory methods for salivary buffering analysis.
abstract: This study evaluated the correlation between three strip-type, colorimetric tests and two laboratory methods with respect to the analysis of salivary buffering. The strip-type tests were saliva-check buffer, Dentobuff strip and CRT(®) Buffer test. The laboratory methods included Ericsson's laboratory method and a monotone acid/base titration to create a reference scale for the salivary titratable acidity. Additionally, defined buffer solutions were prepared and tested to simulate the carbonate, phosphate and protein buffer systems of saliva. The correlation between the methods was analysed by the Spearman's rank test. Disagreement was detected between buffering capacity values obtained with three strip-type tests that was more pronounced in case of saliva samples with medium and low buffering capacities. All strip-type tests were able to assign the hydrogencarbonate, di-hydrogenphosphate and 0.1% protein buffer solutions to the correct buffer categories. However, at 0.6% total protein concentrations, none of the test systems worked accurately. Improvements are necessary for strip-type tests because of certain disagreement with the Ericsson's laboratory method and dependence on the protein content of saliva.
authors.affiliation: Department of Preventive, Restorative and Pediatric Dentistry, University of Bern, Freiburgstrasse 7, 3010, Bern, Switzerland. zeinab.cheaib@zmk.unibe.ch
authors.forename: Zeinab
authors.initials: Z
authors.lastname: Cheaib
authors.forename: Carolina
authors.initials: C
authors.lastname: Ganss
authors.forename: Andreas
authors.initials: A
authors.lastname: Lamanda
authors.forename: Melek Dilek
authors.initials: MD
authors.lastname: Turgut
authors.forename: Adrian
authors.initials: A
authors.lastname: Lussi
mesh_terms: D000328:Adult; D002021:Buffers; D019963:Clinical Chemistry Tests; D003124:Colorimetry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008875:Middle Aged; D011933:Reagent Kits, Diagnostic; D011934:Reagent Strips; D015203:Reproducibility of Results; D012463:Saliva / Q000737:chemistry*; D012471:Salivary Proteins and Peptides / Q000737:chemistry; D017186:Titrimetry
chemical_list: D002021:Buffers; D011933:Reagent Kits, Diagnostic; D011934:Reagent Strips; D012471:Salivary Proteins and Peptides