title: Copper exposure effects on yeast mitochondrial proteome.
abstract: Mitochondria play an important role on the entire cellular copper homeostatic mechanisms. Alteration of cellular copper levels may thus influence mitochondrial proteome and its investigation represents an important contribution to the general understanding of copper-related cellular effects. In these study we have performed an organelle targeted proteomic investigation focusing our attention on the effect of non-lethal 1mM copper concentration on Saccharomyces cerevisiae mitochondrial proteome. Functional copper effects on yeast mitochondrial proteome were evaluated by using both 2D electrophoresis (2-DE) and liquid chromatography coupled with tandem mass spectrometry. Proteomic data have been then analyzed by different unsupervised meta-analysis approaches that highlight the impairment of mitochondrial functions and the activation of oxidative stress response. Interestingly, our data have shown that stress response generated by 1mM copper treatment determines the activation of S. cerevisiae survival pathway. To investigate these findings we have treated yeast cells responsiveness to copper with hydrogen peroxide and observed a protective role of this metal. These results are suggestive of a copper role in the protection from oxidative stress possibly due to the activation of mechanisms involved in cellular survival and growth.
authors.affiliation: Magnetic Resonance Center CERM and Department of Chemistry, University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Florence, Italy.
authors.forename: Lucia
authors.initials: L
authors.lastname: Banci
authors.forename: Ivano
authors.initials: I
authors.lastname: Bertini
authors.forename: Simone
authors.initials: S
authors.lastname: Ciofi-Baffoni
authors.forename: Annamaria
authors.initials: A
authors.lastname: D'Alessandro
authors.forename: Deepa
authors.initials: D
authors.lastname: Jaiswal
authors.forename: Valeria
authors.initials: V
authors.lastname: Marzano
authors.forename: Sara
authors.initials: S
authors.lastname: Neri
authors.forename: Maurizio
authors.initials: M
authors.lastname: Ronci
authors.forename: Andrea
authors.initials: A
authors.lastname: Urbani
mesh_terms: D003300:Copper / Q000494:pharmacology*; D015180:Electrophoresis, Gel, Two-Dimensional; D004781:Environmental Exposure / Q000009:adverse effects; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000032:analysis / Q000378:metabolism*; D008954:Models, Biological; D010084:Oxidation-Reduction; D020543:Proteome / Q000187:drug effects*; D040901:Proteomics / Q000379:methods; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000187:drug effects / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000009:adverse effects / Q000494:pharmacology
chemical_list: D024101:Mitochondrial Proteins; D020543:Proteome; D029701:Saccharomyces cerevisiae Proteins; D014874:Water Pollutants, Chemical; D003300:Copper; D006861:Hydrogen Peroxide