title: Production of interleukin-6 by human osteoclast-like cells from giant cell tumor of bone.
abstract: Giant cell tumor (GCT) is a bone neoplasm which is characterized by the presence of large numbers of multinucleated osteoclast-like giant cells. Although GCT can be considered a benign lesion, it exhibits high local aggressiveness often associated with osteolytic properties. In this study, we used five different GCT primary cell cultures to evaluate whether osteoclast-like cells from GCT are able to produce interleukin-6 (IL-6), a cytokine strictly involved in the induction of osteoclast-mediated bone resorption. IL-6 assessment with ELISA revealed that osteoclast-like GCT cells produce low levels of this cytokine, which can be greatly increased after treatment with both lipopolysaccharide (LPS) or interleukin-1 beta (IL-1 beta). These data were confirmed by molecular analysis which revealed that GCT cells synthesize IL-6 mRNA and that the levels of IL-6 transcripts are greatly increased after treatment with both LPS and IL-1 beta. Moreover, by using a biologic assay with the 7TD1, a IL-6 dependent cell Line, we also determined that IL-6 synthesized by GCT cells is biologically active. This study supports the hypothesis that IL-6 locally released by GCT osteoclast-like cells may be involved in the induction of the osteolysis which is strictly associated with the biologic aggressiveness of GCT cells.
authors.affiliation: UNIV BOLOGNA,IST ISTOL & EMBRIOL,I-40126 BOLOGNA,ITALY. IST ORTOPED RIZZOLI,LAB RICERCA ONCOL,BOLOGNA,ITALY. IST RIC FARMACOL MARIO NEGRI,MILAN,ITALY. CNR,IST TECNOL BIOMED AVANZATE,I-20131 MILAN,ITALY. UNIV FLORENCE,DIPARTIMENTO ANAT UMANA & ISTOL,I-50139 FLORENCE,ITALY. UNIV FLORENCE,DIPARTIMENTO FISIOPATOL CLIN,I-50139 FLORENCE,ITALY. UNIV BOLOGNA,CTR INTERDIPARTIMENTALE RIC CANC G PROD,I-40126 BOLOGNA,ITALY.
authors.forename: M
authors.initials: M
authors.lastname: Giovannini
authors.forename: P
authors.initials: P
authors.lastname: Strippoli
authors.forename: M
authors.initials: M
authors.lastname: Serra
authors.forename: M
authors.initials: M
authors.lastname: Sironi
authors.forename: G
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authors.lastname: Fincato
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authors.initials: F
authors.lastname: Colotta
authors.forename: M
authors.initials: M
authors.lastname: Bonafe
authors.forename: I
authors.initials: I
authors.lastname: Biunno
authors.forename: A
authors.initials: A
authors.lastname: Mantovani
authors.forename: S
authors.initials: S
authors.lastname: Orlandini
authors.forename: M
authors.initials: M
authors.lastname: Brandi
authors.forename: R
authors.initials: R
authors.lastname: Pastano
authors.forename: G
authors.initials: G
authors.lastname: Bagnara