title: Dental caries experience in relation to salivary findings and molecular identification of S. mutans and S. sobrinus in subjects with Down syndrome.
abstract: This study investigated the association between clinical and salivary or molecular parameters in Down syndrome subjects. Sixty individuals (1- to 48-year old) were clinically examined using DMFT/DMFS. Stimulated saliva was collected; salivary flow was calculated (mL/min), buffering capacity was measured using a standard pH tape. In addition, 25 μL of saliva was diluted using 10-fold-dilution method and then placed on Mitis-Salivarius-Bacitracin agar to count colony forming units (CFU/mL) of mutans streptococci. Polymerase chain reaction analysis identified species. Caries indexes were 0.65-13.5 (DMFT) and 0.65-26.0 (DMFS) according to groups. Ninety-four percent of subjects had low flow rate (0.7-1.0 mL/min) and 44% had low buffering capacity (pH < 4). Besides, 60% had more than 1 × 10(6) CFU/mL, 60% had S. mutans, and 41.4% had S. sobrinus. Caries indexes did not significantly correlate with flow rate, buffering capacity, CFU/mL by Pearson's correlation (p > 0.05), and showed no significant association with prevalence of species by Chi-square (p > 0.05). There is no association between clinical picture and salivary or molecular parameters in Down syndrome subjects.
authors.affiliation: Pedodontic Division, Department of Pediatric Dentistry, Piracicaba Dental School, University of Campinas-UNICAMP, P.O. Box 52, Piracicaba, SP, 13414-903, Brazil. acastilho@fop.unicamp.br
authors.forename: Aline Rogéria Freire
authors.initials: AR
authors.lastname: de Castilho
authors.forename: Vanessa
authors.initials: V
authors.lastname: Pardi
authors.forename: Cássio Vicente
authors.initials: CV
authors.lastname: Pereira
mesh_terms: D000293:Adolescent; D000328:Adult; D058491:Bacterial Load; D002021:Buffers; D002648:Child; D002675:Child, Preschool; D004245:DMF Index; D003731:Dental Caries / Q000382:microbiology; D003793:Dental Restoration, Permanent / Q000706:statistics & numerical data; D004314:Down Syndrome / Q000150:complications* / Q000382:microbiology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007223:Infant; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012463:Saliva / Q000378:metabolism / Q000382:microbiology*; D012636:Secretory Rate / Q000502:physiology; D013291:Streptococcus / Q000145:classification; D013295:Streptococcus mutans / Q000145:classification*; D016986:Streptococcus sobrinus / Q000145:classification*; D014081:Tooth Extraction / Q000706:statistics & numerical data; D016388:Tooth Loss / Q000145:classification; D055815:Young Adult
chemical_list: D002021:Buffers