title: Breviscapine attenuates acute pancreatitis by inhibiting expression of PKCα and NF-κB in pancreas.
abstract: OBJECTIVE
  To study the effect of breviscapine (Bre) on activity of protein kinase Cα (PKCα) and nuclear factor (NF)-κB in pancreas, and the mechanism of Bre attenuating acute pancreatitis (AP).
  
  
  METHODS
  One hundred and eight rats were randomly divided into acute necrotizing pancreatitis (ANP) group, Bre group (ANP + Bre group) and sham operation (SO) group, 36 rats in each group. ANP model was induced by a retrograde injection of 4% sodium deoxycholate into the bilio-pancreatic duct. Fifteen minutes after the ANP model was induced, the rats in Bre group were intraperitoneally injected with Bre (0.4 mg/100 g body weight or 0.1 mL/100 g body weight). Survival time and mortality of rats were calculated. Serum amylase and malondialdehyde levels were measured, volume of ascites was recorded and morphology of pancreas and lung was evaluated at 1, 5 and 10 h, after the ANP model was induced, respectively. Expressions of PKCα and subunit p65 of NF-κB in pancreas were detected by immunohistochemistry and Western blotting.
  
  
  RESULTS
  The life span of rats was longer and the mortality was lower in Bre group than in ANP group 13.51 ± 5.46 vs. 25.36 ± 8.11 (P < 0.05). The amylase and MDA levels as well as the volume of ascites were lower and the pathological changes in pancreas and lung were less in Bre group than ANP group (P < 0.05), indicating that the pancreatitis is less severe in Bre group than ANP group. The activation of PKCα and NF-κB p65 in pancreas was induced rapidly and reached their peak at 1 h or 5 h after ANP, but their activity in Bre group was significantly inhibited.
  
  
  CONCLUSIONS
  Bre exerts its therapeutic effect on AP by inhibiting the activation of PKCα and NF-κB p65 in pancreas.
authors.affiliation: Department of Pathophysiology, Shaanxi University of Traditional Chinese Medicine, Xianyang 712046, Shaanxi Province, China. zhangh1227@sohu.com
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.forename: Cui-Zhu
authors.initials: CZ
authors.lastname: Cai
authors.forename: Xiao-Qin
authors.initials: XQ
authors.lastname: Zhang
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.forename: Xiao-Yun
authors.initials: XY
authors.lastname: Jia
authors.forename: Bao-Lan
authors.initials: BL
authors.lastname: Li
authors.forename: Liang
authors.initials: L
authors.lastname: Song
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Ma
mesh_terms: D000818:Animals; D001201:Ascites; D004195:Disease Models, Animal; D004789:Enzyme Activation / Q000187:drug effects; D005419:Flavonoids / Q000494:pharmacology* / Q000627:therapeutic use*; D010179:Pancreas / Q000187:drug effects* / Q000378:metabolism* / Q000473:pathology; D010195:Pancreatitis / Q000188:drug therapy*; D051571:Protein Kinase C-alpha / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015996:Survival Rate; D051996:Transcription Factor RelA / Q000378:metabolism*
chemical_list: D005419:Flavonoids; C498145:Rela protein, rat; D051996:Transcription Factor RelA; C061097:breviscapine; D051571:Protein Kinase C-alpha