title: [Apoptosis inducing effect by TRAIL on human laryngeal squamous carcinoma Hep-2 cells].
abstract: OBJECTIVE
  To study the apoptosis inducing effect of tumor necrosis factor related apoptosis-ligand (TRAIL) on human laryngeal squamous carcinoma Hep-2 cells and its effect mechanism.
  
  
  METHODS
  The human laryngeal squamous carcinoma Hep-2 cell line was treated with different concentration of TRAIL in vitro. The inhibition ratio of tumor cells was determined by MTT colorimetric assay, the incidence of cell apoptosis was determined by flow cytometry method. The morphologic changes of laryngeal squamous carcinoma Hep-2 cell were observed with transmission electron microscope.
  
  
  RESULTS
  In vitro, all the different concentrations of TRAIL inhibited laryngeal squamous carcinoma cell's growth. The inhibited growth ratio showed significant concentration-dependence. The concentrations for inducing apoptosis-ratio(TRAIL 1, 10, 100 microg/L) determined by flow cytometry was (11.49 +/- 0.36)%, (22.31 +/- 0. 82)%, (59.64 +/- 1.10)% respectively in the study group, and (3.13 +/- 0.12)% in the control group, which was significantly different between these two groups (P < 0. 01).
  
  
  CONCLUSIONS
  In vitro, TRAIL inhibited the growth of human laryngeal squamous carcinoma Hep-2 cells. The induced apoptosis of TRAIL shows significant concentration- independence. TRAIL inhibits the growth of human laryngeal squamous carcinoma Hep-2 cells trough inducing apoptosis.
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, the Second Affiliated Hospital of Harbin Medical University, Harbin, 150086, China. yaoyaosworld@yahoo.com.cn
authors.forename: Hongchao
authors.initials: H
authors.lastname: Yao
authors.forename: Hui
authors.initials: H
authors.lastname: Xiao
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.forename: Ming
authors.initials: M
authors.lastname: Liu
authors.forename: Dejun
authors.initials: D
authors.lastname: Jin
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D002294:Carcinoma, Squamous Cell / Q000473:pathology; D045744:Cell Line, Tumor; D006801:Humans; D007822:Laryngeal Neoplasms / Q000473:pathology; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000494:pharmacology*
chemical_list: D053221:TNF-Related Apoptosis-Inducing Ligand