title: Growth factor priming of synovium-derived stem cells for cartilage tissue engineering.
abstract: This study investigated the potential use of synovium-derived stem cells (SDSCs) as a cell source for cartilage tissue engineering. Harvested SDSCs from juvenile bovine synovium were expanded in culture in the presence (primed) or absence (unprimed) of growth factors (1 ng/mL transforming growth factor-β(1), 10 ng/mL platelet-derived growth factor-ββ, and 5 ng/mL basic fibroblast growth factor-2) and subsequently seeded into clinically relevant agarose hydrogel scaffolds. Constructs seeded with growth factor-primed SDSCs that received an additional transient application of transforming growth factor-β(3) for the first 21 days (release) exhibited significantly better mechanical and biochemical properties compared to constructs that received sustained growth factor stimulation over the entire culture period (continuous). In particular, the release group exhibited a Young's modulus (267±96 kPa) approaching native immature bovine cartilage levels, with corresponding glycosaminoglycan content (5.19±1.45%ww) similar to native values, within 7 weeks of culture. These findings suggest that SDSCs may serve as a cell source for cartilage tissue engineering applications.
authors.affiliation: Department of Biomedical Engineering, Columbia University, New York, NY 10027, USA.
authors.forename: Sonal R
authors.initials: SR
authors.lastname: Sampat
authors.forename: Grace D
authors.initials: GD
authors.lastname: O'Connell
authors.forename: Jason V
authors.initials: JV
authors.lastname: Fong
authors.forename: Elena
authors.initials: E
authors.lastname: Alegre-Aguarón
authors.forename: Gerard A
authors.initials: GA
authors.lastname: Ateshian
authors.forename: Clark T
authors.initials: CT
authors.lastname: Hung
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D005434:Flow Cytometry; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects; D013583:Synovial Membrane / Q000166:cytology*; D023822:Tissue Engineering / Q000379:methods*; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology; D053782:Transforming Growth Factor beta3 / Q000494:pharmacology
chemical_list: D010982:Platelet-Derived Growth Factor; D053773:Transforming Growth Factor beta1; D053782:Transforming Growth Factor beta3