title: Upgraded Cobas Ampliprep-Cobas TaqMan version 2.0 HIV-1 RNA quantification assay versus first version: correction of underestimations.
abstract: The large underestimations of HIV RNA quantification observed in 17 patients with the first version of Cobas TaqMan assay have been successfully corrected in the upgraded version 2.0. In comparison with the Abbott RealTime assay, the mean difference that was 1.18 log(10) copies/ml is now zero. The discrepancies have disappeared.
authors.affiliation: Department of Virology, Pitié-Salpêtriere Hospital, Paris, France. marc.wirden@psl.aphp.fr
authors.forename: M
authors.initials: M
authors.lastname: Wirden
authors.forename: R
authors.initials: R
authors.lastname: Tubiana
authors.forename: S
authors.initials: S
authors.lastname: Fourati
authors.forename: M
authors.initials: M
authors.lastname: Thevenin
authors.forename: A
authors.initials: A
authors.lastname: Simon
authors.forename: A
authors.initials: A
authors.lastname: Canestri
authors.forename: Z
authors.initials: Z
authors.lastname: Ait-Arkoub
authors.forename: C
authors.initials: C
authors.lastname: Soulie
authors.forename: A G
authors.initials: AG
authors.lastname: Marcelin
authors.forename: C
authors.initials: C
authors.lastname: Katlama
authors.forename: V
authors.initials: V
authors.lastname: Calvez
mesh_terms: D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D011933:Reagent Kits, Diagnostic; D019562:Viral Load / Q000379:methods*
chemical_list: D012367:RNA, Viral; D011933:Reagent Kits, Diagnostic