title: Hepatoprotective and neuroprotective activity of liposomal quercetin in combating chronic arsenic induced oxidative damage in liver and brain of rats.
abstract: BACKGROUND
  Arsenic is a naturally occurring toxicant that causes acute and chronic adverse health effects, including cancer.
  
  
  OBJECTIVE
  The study was performed to evaluate the therapeutic efficacy of liposome entrapped flavonoidal quercetin in combating arsenic toxicity mediated oxidative damage in hepatocytes and brain cells in rat model.
  
  
  METHODS
  Hepatic and neuronal cell damage in rats was made by daily arsenic (6 mg/kg b wt, 9 mg/kg b wt and 12 mg/kg b wt) treatment via oral route for four consecutive months. Liposomal quercetin (2.71 mg QC/kg b. wt) were injected s.c. on rats treated with 12 mg/kg b. wt. NaAsO(2) twice a week for four months.
  
  
  CONCLUSIONS
  Inorganic arsenic deposition was found to be most significant in hepatic (9.32 ± 0.100 µg/g tissue) and neuronal (6.21 ± 0.090 µg/g tissue) cells of rats treated with 12 mg/kg b wt of arsenite. Antioxidant levels in hepatic and neuronal cells were reduced significantly by the induction of arsenic. Liposomal quercetin was found most potent for a complete prevention of arsenite-induced reduction in antioxidant levels in the liver and brain of rats. Arsenic induced a substantial increase in hepatic hydroxyproline (HP) and Liposomal quercetin treatment resulted in complete replenishment of the HP level to normal. Liposomal quercetin completely prevented the arsenite-induced upregulation of cytochrome c expression in liver and brain significantly suggesting that the protective effect of Liposomal quercetin could be related to the reduction of arsenic deposition in both the organs.
  
  
  CONCLUSIONS
  Thus, Liposomal quercetin might prove to be of therapeutic potential against arsenite-induced hepatic and neuronal cell damage in rats.
authors.affiliation: Biomembrane Division, Indian Institute of Chemical Biology, 4, Raja S.C. Mullick Road, Kolkata-700032, India.
authors.forename: Aparajita
authors.initials: A
authors.lastname: Ghosh
authors.forename: Ardhendu K
authors.initials: AK
authors.lastname: Mandal
authors.forename: Sibani
authors.initials: S
authors.lastname: Sarkar
authors.forename: Nirmalendu
authors.initials: N
authors.lastname: Das
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001151:Arsenic / Q000633:toxicity; D001921:Brain / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D045304:Cytochromes c / Q000378:metabolism; D005260:Female; D008081:Liposomes / Q000008:administration & dosage*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects*; D011794:Quercetin / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats
chemical_list: D000975:Antioxidants; D008081:Liposomes; D045304:Cytochromes c; D011794:Quercetin; D001151:Arsenic