title: Identification of arginine residues in peptides by 2D-IR echo spectroscopy.
abstract: The CN stretching vibrations of the guanidyl group in the arginine dipeptide side chain are examined by two-dimensional infrared spectroscopy. In D(2)O, the spectra display two distinct diagonal peaks. These nearly degenerate modes undergo ultrafast energy transfer. The energy-transfer rate was determined directly from the 2D-IR spectra to be 1/2.1 ps(-1). The cross peaks in 2D-IR arising from the energy transfer provide a definitive identification of arginine in larger proteins. An example of arginine in the transmembrane protein M2, found in influenza viruses, is given.
authors.affiliation: Department of Chemistry, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6323, USA.
authors.forename: Ayanjeet
authors.initials: A
authors.lastname: Ghosh
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Tucker
authors.forename: Robin M
authors.initials: RM
authors.lastname: Hochstrasser
mesh_terms: D001120:Arginine / Q000032:analysis* / Q000737:chemistry; D002627:Chemistry, Physical; D017666:Deuterium Oxide / Q000737:chemistry; D004151:Dipeptides / Q000032:analysis / Q000737:chemistry*; D004735:Energy Transfer; D019791:Guanidine / Q000737:chemistry; D007700:Kinetics; D017433:Protein Structure, Secondary; D013055:Spectrophotometry, Infrared; D017550:Spectroscopy, Fourier Transform Infrared; D014732:Vibration; D014763:Viral Matrix Proteins / Q000032:analysis / Q000737:chemistry*; D000086082:Viroporin Proteins
chemical_list: D001120:Arginine; D017666:Deuterium Oxide; D004151:Dipeptides; D019791:Guanidine; D014763:Viral Matrix Proteins; C488937:M2 protein, Influenza A virus; D000086082:Viroporin Proteins