title: Effects of immunization with the rNfa1 protein on experimental Naegleria fowleri-PAM mice.
abstract: Free-living Naegleria fowleri causes primary amoebic meningoencephalitis (PAM) in humans and animals. To examine the effect of immunization with Nfa1 protein on experimental murine PAM because of N. fowleri, BALB/c mice were intra-peritoneally or intra-nasally immunized with a recombinant Nfa1 protein. We analysed Nfa1-specific antibody and cytokine induction, and the mean survival time of infected mice. Mice immunized intra-peritoneally or intra-nasally with rNfa1 protein developed specific IgG, IgA and IgE antibodies; the IgG response was dominated by IgG1, followed by IgG2b, IgG2a and IgG3. High levels of the Th1 cytokine, IFN-γ, and the regulatory cytokine, IL-10, were also induced. The mean survival time of mice immunized intra-peritoneally with rNfa1 protein was prolonged compared with controls, (25.0 and 15.5 days, respectively). Similarly, the mean survival time of mice immunized intra-nasally with rNfa1 protein was 24.7 days, compared with 15.0 days for controls.
authors.affiliation: Department of Microbiology, and Molecular Science and Technology, Ajou University School of Medicine, Suwon, Korea.
authors.forename: Y J
authors.initials: YJ
authors.lastname: Lee
authors.forename: J H
authors.initials: JH
authors.lastname: Kim
authors.forename: H J
authors.initials: HJ
authors.lastname: Sohn
authors.forename: J
authors.initials: J
authors.lastname: Lee
authors.forename: S Y
authors.initials: SY
authors.lastname: Jung
authors.forename: Y J
authors.initials: YJ
authors.lastname: Chwae
authors.forename: K
authors.initials: K
authors.lastname: Kim
authors.forename: S
authors.initials: S
authors.lastname: Park
authors.forename: H J
authors.initials: HJ
authors.lastname: Shin
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000953:Antigens, Protozoan / Q000235:genetics / Q000276:immunology*; D020808:Central Nervous System Protozoal Infections / Q000276:immunology / Q000517:prevention & control*; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D007070:Immunoglobulin A / Q000097:blood; D007073:Immunoglobulin E / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007274:Injections, Intraperitoneal; D007963:Leukocytes, Mononuclear / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D016848:Naegleria fowleri / Q000276:immunology* / Q000472:pathogenicity; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology*; D016052:Protozoan Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D012376:Rodent Diseases / Q000517:prevention & control; D013154:Spleen / Q000276:immunology; D016019:Survival Analysis; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D016207:Cytokines; D007070:Immunoglobulin A; D007074:Immunoglobulin G; C460560:Nfa1 protein, Naegleria fowleri; D015800:Protozoan Proteins; D016052:Protozoan Vaccines; D014614:Vaccines, Synthetic; D007073:Immunoglobulin E