title: End-binding proteins EB3 and EB1 link microtubules to ankyrin G in the axon initial segment.
abstract: The axon initial segment (AIS) plays a key role in maintaining the molecular and functional polarity of the neuron. The relationship between the AIS architecture and the microtubules (MTs) supporting axonal transport is unknown. Here we provide evidence that the MT plus-end-binding (EB) proteins EB1 and EB3 have a role in the AIS in addition to their MT plus-end tracking protein behavior in other neuronal compartments. In mature neurons, EB3 is concentrated and stabilized in the AIS. We identified a direct interaction between EB3/EB1 and the AIS scaffold protein ankyrin G (ankG). In addition, EB3 and EB1 participate in AIS maintenance, and AIS disassembly through ankG knockdown leads to cell-wide up-regulation of EB3 and EB1 comets. Thus, EB3 and EB1 coordinate a molecular and functional interplay between ankG and the AIS MTs that supports the central role of ankG in the maintenance of neuronal polarity.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale, Unité Mixte de Recherche 641, F-13916 Marseille, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Leterrier
authors.forename: Hélène
authors.initials: H
authors.lastname: Vacher
authors.forename: Marie-Pierre
authors.initials: MP
authors.lastname: Fache
authors.forename: Stéphanie Angles
authors.initials: SA
authors.lastname: d'Ortoli
authors.forename: Francis
authors.initials: F
authors.lastname: Castets
authors.forename: Amapola
authors.initials: A
authors.lastname: Autillo-Touati
authors.forename: Bénédicte
authors.initials: B
authors.lastname: Dargent
mesh_terms: D000818:Animals; D017487:Ankyrins / Q000378:metabolism*; D001369:Axons / Q000378:metabolism*; D016764:Cell Polarity; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism*; D009474:Neurons / Q000648:ultrastructure; D011485:Protein Binding; D051381:Rats; D017208:Rats, Wistar
chemical_list: C490614:Ank3 protein, rat; D017487:Ankyrins; C561584:Mapre1 protein, rat; C534832:Mapre3 protein, rat; D008869:Microtubule-Associated Proteins