title: Glucosaminylmuramyldipeptide-induced changes in phenotype of melanoma cells result in their increased lysis by peripheral blood cells.
abstract: Flow cytometry was used to show that biologically active N-acetylglucosamine-containing muramylpeptides (GMPs) induced in vitro dose-dependent increase in the expression of tumor-associated antigens (TAAs) characteristic for colon and mammary gland carcinomas, melanoma and lung adenocarcinoma. Forty to two hundred percent enhancement in TAA-expressing cells was observed after 18-48 h incubation with GMPs. In contrast, MHC class I antigen expression was not altered. Using MTT and chromium-release assays, melanoma cells treated in vitro with GMDP were shown to be more susceptible to killing by peripheral blood cells of healthy donors than non-treated cells. Fractionation of blood cells revealed that platelets were responsible for this effect.
authors.affiliation: MM SHEMYAKIN INST BIOORGAN CHEM,MOSCOW 117871,RUSSIA. RUSSIAN ACAD MED SCI,CTR CANC RES,MOSCOW 115478,RUSSIA.
authors.forename: T
authors.initials: T
authors.lastname: Valyakina
authors.forename: A
authors.initials: A
authors.lastname: Malakhov
authors.forename: N
authors.initials: N
authors.lastname: Malakhova
authors.forename: E
authors.initials: E
authors.lastname: Petrova
authors.forename: S
authors.initials: S
authors.lastname: Bykovskaya
authors.forename: E
authors.initials: E
authors.lastname: Revazova
authors.forename: V
authors.initials: V
authors.lastname: Nesmeyanov