title: Multiple target loci assembly sequencing (mTAS).
abstract: Here we present multiple target loci assembly sequencing (mTAS), a method for examining multiple genomic loci in a single DNA sequencing read. The key to the success of mTAS target sequencing is the uniform amplification of multiple target genomic loci into a single DNA fragment using polymerase cycling assembly (PCA). Using this strategy, we successfully collected multiloci sequence information from a single DNA sequencing run. We applied mTAS to examine 29 different sets of human genomic loci, each containing from 2 to 11 single-nucleotide polymorphisms (SNP) present at different exons. We believe mTAS can be used to reduce the cost of Sanger sequencing-based genetic analysis.
authors.affiliation: Department of Chemistry, Yonsei University, Shinchon 134, Seoul 120-749, Republic of Korea.
authors.forename: Hyojun
authors.initials: H
authors.lastname: Han
authors.forename: Jung-Ki
authors.initials: JK
authors.lastname: Yoon
authors.forename: Byoung Chul
authors.initials: BC
authors.lastname: Cho
authors.forename: Hwangbeom
authors.initials: H
authors.lastname: Kim
authors.forename: Duhee
authors.initials: D
authors.lastname: Bang
mesh_terms: D005091:Exons; D056426:Genetic Loci; D015894:Genome, Human; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA / Q000379:methods*