title: Antibacterial efficacy of endodontic irrigating solutions and their combinations in root canals contaminated with Enterococcus faecalis.
abstract: OBJECTIVE
  The objective of this study was to evaluate the antibacterial efficacy of irrigating solutions and their combinations against Enterococcus faecalis.
  
  
  METHODS
  One hundred ten single-rooted human teeth were inoculated with E. faecalis and incubated for 21 days. Teeth were divided according to the irrigant: Group I (GI), 2.5% sodium hypochlorite solution (NaOCl); GII, 2.5% NaOCl + 10% citric acid; GIII, 2.5% NaOCl + apple cider vinegar; GIV, apple cider vinegar; GV, 2% chlorhexidine solution; GVI, 1% peracetic acid; GVII, saline solution. Microbiological samples were taken after root canal preparation and 7 days later. Data were submitted to ANOVA (5%).
  
  
  RESULTS
  All solutions promoted reduction of E. faecalis after instrumentation, but bacterial counts were higher in the final sample. GI, GV, and GVI had lower bacterial counts than the other groups.
  
  
  CONCLUSIONS
  The irrigating solutions may present activity but do not eradicate E. faecalis in the root canal system.
authors.affiliation: Araraquara Dental School, UNESP, São Paulo State University, Department of Restorative Dentistry, Araraquara, SP, Brazil.
authors.forename: Renata
authors.initials: R
authors.lastname: Dornelles-Morgental
authors.forename: Juliane Maria
authors.initials: JM
authors.lastname: Guerreiro-Tanomaru
authors.forename: Norberto Batista
authors.initials: NB
authors.lastname: de Faria-Júnior
authors.forename: Marco Antonio
authors.initials: MA
authors.lastname: Hungaro-Duarte
authors.forename: Milton Carlos
authors.initials: MC
authors.lastname: Kuga
authors.forename: Mário
authors.initials: M
authors.lastname: Tanomaru-Filho
mesh_terms: D000704:Analysis of Variance; D000891:Anti-Infective Agents, Local / Q000737:chemistry / Q000627:therapeutic use*; D015169:Colony Count, Microbial; D003786:Dental Pulp Cavity / Q000187:drug effects / Q000382:microbiology*; D013293:Enterococcus faecalis / Q000187:drug effects*; D006801:Humans; D012388:Root Canal Irrigants / Q000737:chemistry / Q000627:therapeutic use*
chemical_list: D000891:Anti-Infective Agents, Local; D012388:Root Canal Irrigants