title: Determination of atenolol in human urine by gas chromatography-mass spectrometry method.
abstract: A sensitive and efficient method was developed for the determination of atenolol in human urine by gas chromatography-mass spectrometry (GC-MS). Atenolol and metoprolol (internal standard, IS) were extracted from human urine with a mixture of chloroform and butanol at basic pH with liquid-liquid extraction. The extracts were derivatized with N-Methyl-N-(trimethylsilyl)trifluoroacetamide (MSTFA) and analyzed by GC-MS using a capillary column. The standard curve was linear (r = 0.99) over the concentration range of 50-750 ng/mL. Intra- and inter-day precision, expressed as the relative standard deviation were less than 5.0%, and accuracy (relative error) was better than 7.0%. The analytical recovery of atenolol from human urine has averaged 91%. The limit of quantification was 50 ng/mL. Also, the method was successfully applied to a patient with hypertension who had been given an oral tablet of 50 mg atenolol.
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, Ataturk University, 25240, Erzurum, Turkey. yilmazb@atauni.edu.tr
authors.forename: Bilal
authors.initials: B
authors.lastname: Yilmaz
authors.forename: Sakir
authors.initials: S
authors.lastname: Arslan
mesh_terms: D000328:Adult; D000959:Antihypertensive Agents / Q000302:isolation & purification / Q000652:urine; D001262:Atenolol / Q000302:isolation & purification / Q000652:urine*; D000440:Butanols / Q000737:chemistry; D002725:Chloroform / Q000737:chemistry; D004355:Drug Stability; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D016014:Linear Models; D008297:Male; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000959:Antihypertensive Agents; D000440:Butanols; D001262:Atenolol; D002725:Chloroform