title: Quantitative determination and pharmacokinetic study of solamargine in rat plasma by liquid chromatography-mass spectrometry.
abstract: A sensitive and simple liquid chromatography-mass spectrometry (LC-MS) method has been developed and validated for the quantification of solamargine, a steroidal glycoalkaloid, in rat plasma. Vincristine was selected as the internal standard. Sample preparation involved simple liquid-liquid extraction by ethyl acetate with high efficiency. The chromatographical separation was performed on a Shimadzu C(18) column (150mm×2.0mm, 5μm) with a gradient elution of acetonitrile and 0.02% (v/v) formic acid. The elutes were detected under positive electrospray ionization (ESI) and the target analytes quantified by selected ion monitoring (SIM) mode. The method was sensitive with the lowest limit of quantitation (LLOQ) at 0.5ng/mL in 50μL of rat plasma. Good linearity (r(2)=0.9996) was obtained covering the concentration of 0.5-2000.0ng/mL. The intra- and inter-day assay precision ranged from 2.87 to 3.60% and 0.52 to 6.81%, respectively. In addition, the stability, extraction recovery and matrix effect involved in the method were also validated. The practical utility of the aforementioned method was successfully confirmed in the pharmacokinetic evaluation of solamargine in Sprague-Dawley rats after intravenous administration.
authors.affiliation: Key Lab of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, No. 24 Tongjiaxiang Street, Nanjing 210009, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Zheng
authors.forename: Lianming
authors.initials: L
authors.lastname: Xu
authors.forename: Yan
authors.initials: Y
authors.lastname: Liang
authors.forename: Wei
authors.initials: W
authors.lastname: Xiao
authors.forename: Lin
authors.initials: L
authors.lastname: Xie
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Zhao
authors.forename: Liang
authors.initials: L
authors.lastname: Cao
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.forename: Guangji
authors.initials: G
authors.lastname: Wang
mesh_terms: D000818:Animals; D002138:Calibration; D002623:Chemistry Techniques, Analytical; D002626:Chemistry, Pharmaceutical / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods*; D005561:Formates / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D008956:Models, Chemical; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012991:Solanaceous Alkaloids / Q000493:pharmacokinetics*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D014750:Vincristine / Q000737:chemistry
chemical_list: D005561:Formates; D012991:Solanaceous Alkaloids; C030544:formic acid; C026609:beta-solamarine; D014750:Vincristine