title: The CYP701B1 of Physcomitrella patens is an ent-kaurene oxidase that resists inhibition by uniconazole-P.
abstract: The moss Physcomitrella patens produces both ent-kaurene and ent-kaurenoic acid, which are intermediates of gibberellin biosynthesis in flowering plants. The CYP701 superfamily of cytochrome P450s functions as ent-kaurene oxidases in the biosynthesis of ent-kaurenoic acid. A candidate gene encoding ent-kaurene oxidase in P. patens, CYP701B1, was cloned and heterologously expressed in yeast to examine enzyme activities in vitro. The recombinant CYP701B1 protein catalyzed the oxidation reaction from ent-kaurene to ent-kaurenoic acid. CYP701B1 activity was highly resistant to the ent-kaurene oxidase inhibitor uniconazole-P (IC(50) 64 μM), even though the activity of Arabidopsis ent-kaurene oxidase (CYP701A3) was sensitive (IC(50) 0.26 μM).
authors.affiliation: United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Tokyo, Japan.
authors.forename: Sho
authors.initials: S
authors.lastname: Miyazaki
authors.forename: Takumi
authors.initials: T
authors.lastname: Katsumata
authors.forename: Masahiro
authors.initials: M
authors.lastname: Natsume
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kawaide
mesh_terms: D017360:Arabidopsis / Q000201:enzymology; D019068:Bryopsida / Q000201:enzymology*; D065607:Cytochrome P-450 Enzyme Inhibitors; D003577:Cytochrome P-450 Enzyme System; D004224:Diterpenes / Q000378:metabolism; D045786:Diterpenes, Kaurane / Q000378:metabolism; D005875:Gibberellins / Q000096:biosynthesis; D010940:Plant Proteins; D014230:Triazoles / Q000494:pharmacology*
chemical_list: D065607:Cytochrome P-450 Enzyme Inhibitors; D004224:Diterpenes; D045786:Diterpenes, Kaurane; D005875:Gibberellins; D010940:Plant Proteins; D014230:Triazoles; C511205:uniconazole-P; C002627:kaurene; C051436:kaurenoic acid; D003577:Cytochrome P-450 Enzyme System; C066398:ent-kaurene oxidase