title: Ntann12 annexin expression is induced by auxin in tobacco roots.
abstract: Ntann12, encoding a polypeptide homologous to annexins, was found previously to be induced upon infection of tobacco with the bacterium Rhodococcus fascians. In this study, Ntann12 is shown to bind negatively charged phospholipids in a Ca(2+)-dependent manner. In plants growing in light conditions, Ntann12 is principally expressed in roots and the corresponding protein was mainly immunolocalized in the nucleus. Ntann12 expression was inhibited following plant transfer to darkness and in plants lacking the aerial part. However, an auxin (indole-3-acetic acid) treatment restored the expression of Ntann12 in the root system in dark conditions. Conversely, polar auxin transport inhibitors such as 1-naphthylphthalamic acid (NPA) or 2,3,5-triiodobenzoic acid (TIBA) inhibited Ntann12 expression in light condition. These results indicate that the expression of Ntann12 in the root is linked to the perception of a signal in the aerial part of the plant that is transmitted to the root via polar auxin transport.
authors.affiliation: Université Libre de Bruxelles, B-6041 Gosselies, Belgium. mbaucher@ulb.ac.be
authors.forename: Marie
authors.initials: M
authors.lastname: Baucher
authors.forename: Yves
authors.initials: Y
authors.lastname: Oukouomi Lowe
authors.forename: Olivier M
authors.initials: OM
authors.lastname: Vandeputte
authors.forename: Johnny
authors.initials: J
authors.lastname: Mukoko Bopopi
authors.forename: Jihad
authors.initials: J
authors.lastname: Moussawi
authors.forename: Marjorie
authors.initials: M
authors.lastname: Vermeersch
authors.forename: Adeline
authors.initials: A
authors.lastname: Mol
authors.forename: Mondher
authors.initials: M
authors.lastname: El Jaziri
authors.forename: Fabrice
authors.initials: F
authors.lastname: Homblé
authors.forename: David
authors.initials: D
authors.lastname: Pérez-Morga
mesh_terms: D017302:Annexins / Q000378:metabolism*; D003624:Darkness; D007210:Indoleacetic Acids / Q000037:antagonists & inhibitors / Q000494:pharmacology; D008027:Light; D010743:Phospholipids / Q000378:metabolism; D010797:Phthalimides / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000037:antagonists & inhibitors / Q000494:pharmacology; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism* / Q000528:radiation effects; D015398:Signal Transduction; D014026:Nicotiana / Q000254:growth & development / Q000378:metabolism* / Q000528:radiation effects; D014283:Triiodobenzoic Acids / Q000494:pharmacology
chemical_list: D017302:Annexins; D007210:Indoleacetic Acids; D010743:Phospholipids; D010797:Phthalimides; D010937:Plant Growth Regulators; D014283:Triiodobenzoic Acids; C002687:alpha-naphthylphthalamic acid; C030737:indoleacetic acid; C052968:2,3,5-triiodobenzoic acid