title: Identification of serine/threonine kinase and nucleotide-binding site-leucine-rich repeat (NBS-LRR) genes in the fire blight resistance quantitative trait locus of apple cultivar 'Evereste'.
abstract: Fire blight is the most destructive bacterial disease affecting apple (Malus×domestica) worldwide. So far, no resistance gene against fire blight has been characterized in apple, despite several resistance regions having been identified. A highly efficacious resistance quantitative trait locus (QTL) was localized on linkage group 12 (LG12) of the ornamental cultivar 'Evereste'. A marker previously reported to be closely linked to this resistance was used to perform a chromosome landing. A bacterial artificial chromosome (BAC) clone of 189 kb carrying the fire blight resistance QTL was isolated and sequenced. New microsatellite markers were developed, and the genomic region containing the resistance locus was limited to 78 kb. A cluster of eight genes with homologies to already known resistance gene structures to bacterial diseases was identified and the corresponding gene transcription was verified. From this cluster, two genes were recognized in silico as the two most probable fire blight resistance genes showing homology with the Pto/Prf complex in tomato.
authors.affiliation: Plant Pathology, Institute of Integrative Biology, ETH Zurich, Universitaetstrasse 2, CH 8092 Zurich, Switzerland.
authors.forename: Gabriella
authors.initials: G
authors.lastname: Parravicini
authors.forename: Cesare
authors.initials: C
authors.lastname: Gessler
authors.forename: Caroline
authors.initials: C
authors.lastname: Denancé
authors.forename: Pauline
authors.initials: P
authors.lastname: Lasserre-Zuber
authors.forename: Emilie
authors.initials: E
authors.lastname: Vergne
authors.forename: Marie-Noëlle
authors.initials: MN
authors.lastname: Brisset
authors.forename: Andrea
authors.initials: A
authors.lastname: Patocchi
authors.forename: Charles-Eric
authors.initials: CE
authors.lastname: Durel
authors.forename: Giovanni A L
authors.initials: GA
authors.lastname: Broggini
mesh_terms: D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D007113:Immunity, Innate / Q000235:genetics / Q000502:physiology; D027845:Malus / Q000382:microbiology*; D010935:Plant Diseases / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D040641:Quantitative Trait Loci
chemical_list: D010940:Plant Proteins; D017346:Protein Serine-Threonine Kinases