title: The tumorigenic phenotype of a mutated form of Fc gamma RIIB1, lacking the ability to generate soluble receptor and allowing a low-level of ligand binding.
abstract: Non immunohematopoietic murine tumor cells ectopically expressing Fc gamma RIIB1 (B1) were recently shown to express a higher tumorigenicity phenotype than cells not expressing this receptor. Utilizing a genetic approach we studied the possible contribution of a soluble form of B1 to tumor enhancement. A mutated form of the B1, lacking the cleavage site responsible for the generation of soluble B1 was produced using gene splicing by overlap extension PCR. A deletion confirmed by sequence analysis from 172 to 178 residues was generated. Stable transfectants expressed the B1 deleted form (B1 Delta) both as specific RNA and as a membrane protein receptor allowing a low level of ligand binding. The soluble form of B1 was undetectable in tissue culture supernatants of Bib transfected cells while it was present in supernatants of wild type B1-transfectants. Stable B1 Delta transfectants were significantly more tumorigenic than negative control transfectants. Tumor incidence was almost as high as that of intact B1 and lagged in the latency period before the appearance of palpable tumors. It is suggested that the soluble B1 has a minimal contribution to tumor enhancement.
authors.affiliation: UNIV PENN,SCH MED,IMMUNOL GRAD GRP,PHILADELPHIA,PA 19104.
authors.forename: M
authors.initials: M
authors.lastname: Ran
authors.forename: T
authors.initials: T
authors.lastname: Zusman
authors.forename: E
authors.initials: E
authors.lastname: Lisansky
authors.forename: M
authors.initials: M
authors.lastname: Eskenasy
authors.forename: R
authors.initials: R
authors.lastname: Eshel
authors.forename: Y
authors.initials: Y
authors.lastname: Avivi
authors.forename: Z
authors.initials: Z
authors.lastname: Indik
authors.forename: A
authors.initials: A
authors.lastname: Schreiber