title: Interplay between glycogen synthase kinase-3β and tau in the cerebellum of Hsp27 transgenic mouse.
abstract: The association between heat shock protein 27 (Hsp27) and hyperphosphorylated tau has gained attention for more than a decade, but it has never been explored in vivo. In the present study, we found that tau phosphorylated at S396/404 (PHF-1) and S262 sites was significantly increased in the cerebellum of Hsp27 transgenic mice, which was concomitant with increased glycogen synthase kinase-3β (GSK3β) phosphorylated at Y216 and decreased GSK3β phosphorylated at S9. Neither 70-kDa ribosomal protein S6 kinase (p70S6K; total p70S6K, p70S6K at T389, and p70S6K at T421/S424) nor protein phosphatase PP2A (total PP2A, PP2A at Y307, methylated or demethylated PP2A) was changed. This suggests that the increased tau phosphorylation at S396/404 and S262 sites may be induced by Hsp27 through enhancement of GSK3β activity in the mouse cerebellum.
authors.affiliation: Karolinska Institutet, Department of Neurobiology, Care Sciences and Society, KI-Alzheimer's Disease Research Center, Novum, Huddinge, Sweden.
authors.forename: Shan
authors.initials: S
authors.lastname: Wang
authors.forename: Melinda Erzsebet
authors.initials: ME
authors.lastname: Toth
authors.forename: Erika
authors.initials: E
authors.lastname: Bereczki
authors.forename: Miklos
authors.initials: M
authors.lastname: Santha
authors.forename: Zhi-Zhong
authors.initials: ZZ
authors.lastname: Guan
authors.forename: Bengt
authors.initials: B
authors.lastname: Winblad
authors.forename: Jin-Jing
authors.initials: JJ
authors.lastname: Pei
mesh_terms: D000818:Animals; D002531:Cerebellum / Q000378:metabolism*; D038362:Glycogen Synthase Kinase 3 / Q000378:metabolism*; D000071679:Glycogen Synthase Kinase 3 beta; D055551:HSP27 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D010766:Phosphorylation; D016875:tau Proteins / Q000378:metabolism*
chemical_list: D055551:HSP27 Heat-Shock Proteins; D016875:tau Proteins; D000071679:Glycogen Synthase Kinase 3 beta; C000605507:Gsk3b protein, mouse; D038362:Glycogen Synthase Kinase 3