title: Roles of sequential ubiquitination of PCNA in DNA-damage tolerance.
abstract: Living organisms not only repair DNA damage induced by environmental agents and endogenous cellular metabolites, but have also developed mechanisms to survive in the presence of otherwise lethal lesions. DNA-damage tolerance (DDT) is considered such a mechanism that resumes DNA synthesis in the presence of replication-blocking lesions. Recent studies revealed that DDT in budding yeast is achieved through sequential ubiquitination of DNA polymerase processivity factor, proliferating cell nuclear antigen (PCNA). It is generally believed that monoubiquitinated PCNA promotes translesion DNA synthesis, whereas polyubiquitinated PCNA mediates an error-free mode of lesion bypass. This review will discuss how ubiquitinated PCNA modulates different means of lesion bypass.
authors.affiliation: College of Life Sciences, Capital Normal University, Beijing 100048, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Zhang
authors.forename: Zhoushuai
authors.initials: Z
authors.lastname: Qin
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Xiao
mesh_terms: D000818:Animals; D004247:DNA / Q000235:genetics / Q000378:metabolism; D004249:DNA Damage; D004261:DNA Replication; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D006801:Humans; D008957:Models, Genetic; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D054875:Ubiquitination
chemical_list: D018809:Proliferating Cell Nuclear Antigen; D004247:DNA; D004259:DNA-Directed DNA Polymerase