title: Characterization of a monoclonal antibody to erythroid related factor.
abstract: We describe here a novel IgG monoclonal antibody to erythroid-related factor (ERAF), also known as alpha hemoglobin stabilizing protein (AHSP) and eryththroid differentiation related factor (EDRF). Our antibody named PCE 5 is an IgG(1) kappa chain and is to the peptide sequence MVTVVE ranked highly in our active site analysis and binds with high affinity to ERAF.
authors.affiliation: Scottish National Blood Transfusion Service, National Science Laboratory, Components R&D section, Edinburgh, United Kingdom. alex.Morrison@nhs.net
authors.forename: Alex
authors.initials: A
authors.lastname: Morrison
authors.forename: Kim
authors.initials: K
authors.lastname: Wilson
authors.forename: Loraine
authors.initials: L
authors.lastname: McMillan
authors.forename: Ian
authors.initials: I
authors.lastname: MacGregor
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology / Q000302:isolation & purification; D001665:Binding Sites / Q000276:immunology*; D001798:Blood Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D018604:Epitope Mapping; D006453:Hemoglobinopathies / Q000175:diagnosis; D006801:Humans; D006825:Hybridomas / Q000276:immunology / Q000378:metabolism; D007074:Immunoglobulin G / Q000737:chemistry* / Q000378:metabolism; D007132:Immunoglobulin Isotypes / Q000032:analysis; D007145:Immunoglobulin kappa-Chains / Q000032:analysis; D051379:Mice; D018832:Molecular Chaperones / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D009101:Multiple Myeloma; D011485:Protein Binding / Q000276:immunology; D011817:Rabbits; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D012756:Sheep
chemical_list: C468768:AHSP protein, human; D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D001798:Blood Proteins; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D007145:Immunoglobulin kappa-Chains; D018832:Molecular Chaperones; D011994:Recombinant Proteins