title: Lowfat, a mammalian Lix1 homologue, regulates leg size and growth under the Dachsous/Fat signaling pathway during tissue regeneration.
abstract: In the cricket Gryllus bimaculatus, missing distal parts of amputated legs are regenerated from blastemas based on positional information. The Dachsous/Fat (Ds/Ft) signaling pathway regulates blastema cell proliferation and positional information along the longitudinal axis during leg regeneration. Herein, we show that the Gryllus homologue of Lowfat (Gb'Lft), which modulates Ds/Ft signaling in Drosophila, is involved in leg regeneration. Gb'lft is expressed in regenerating legs, and RNAi against Gb'lft (Gb'lft(RNAi)) suppressed blastema cell hyperproliferation caused by Gb'ft(RNAi) or Gb'ds(RNAi) but enhanced that caused by Gb'kibra(RNAi) or Gb'warts(RNAi). In Gb'lft(RNAi) nymphs, missing parts of amputated legs were regenerated, but the length of the regenerated legs was shortened depending on the position of the amputation. Both normal and reversed intercalary regeneration occurred in Gb'lft(RNAi) nymphs, suggesting that Gb'Lft is involved in blastema cell proliferation and longitudinal leg regeneration under the Ds/Ft signaling pathway, but it is not required for intercalary regeneration.
authors.affiliation: Department of Life Systems, Institute of Technology and Science, The University of Tokushima Graduate School, Tokushima City, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Bando
authors.forename: Yoshimasa
authors.initials: Y
authors.lastname: Hamada
authors.forename: Kazuki
authors.initials: K
authors.lastname: Kurita
authors.forename: Taro
authors.initials: T
authors.lastname: Nakamura
authors.forename: Taro
authors.initials: T
authors.lastname: Mito
authors.forename: Hideyo
authors.initials: H
authors.lastname: Ohuchi
authors.forename: Sumihare
authors.initials: S
authors.lastname: Noji
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D030801:Animals, Genetically Modified; D000071183:Autophagy-Related Proteins; D015820:Cadherins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D006135:Gryllidae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism / Q000502:physiology; D006614:Hindlimb / Q000254:growth & development* / Q000502:physiology; D006801:Humans; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D008322:Mammals / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009929:Organ Size / Q000235:genetics; D010802:Phylogeny; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D012038:Regeneration / Q000235:genetics* / Q000502:physiology; D017385:Sequence Homology; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction / Q000502:physiology
chemical_list: D000071183:Autophagy-Related Proteins; D015820:Cadherins; D015815:Cell Adhesion Molecules; D019476:Insect Proteins; C530882:Lix1 protein, human; D016601:RNA-Binding Proteins