title: Tanshinone IIa alleviates the biochemical changes associated with hypoxic ischemic brain damage in a rat model.
abstract: This study aimed to investigate the effects of Tanshinone IIa (TanIIa) on the biochemical changes associated with hypoxic ischemic brain damage (HIBD) in a rat model. Neonatal SD rats were randomized into normal control, HIBD and TanIIa + HIBD groups. At different time points after HIBD, TanIIa was given at 1 µg/g. The intracellular free calcium concentration and the expression of phospho-NR1 S897 was determined. The intracellular free calcium concentration in the HIBD group was significantly increased. The induction of intracellular free calcium concentration in the TanIIa + HIBD group was less than that in the HIBD group. Large amounts of phospho-NR1 S897 positive cells were distributed in the cortex in the normal control group; the number of phospho-NR1 S897 positive cells in the ipsilateral cortex was dramatically decreased at 24 h after HIBD. Both the number of phospho-NR1 S897 positive cells and the FITC fluorescent density in the HIBD + TanIIa group were less than those in the normal control group at every time point after HIBD, but more than those in the HIBD group. TanIIa alleviated the down-regulation of phospho-NR1 S897 and the elevated intracellular free calcium concentration in the cerebral cortex in the HIBD model. TanIIa could exert a neuroprotective effect through affecting NMDA receptor expression, inhibiting calcium transportation and decreasing the intracellular free calcium concentration.
authors.affiliation: Department of Pediatrics, The 3rd Xiangya Hospital of Central South University, Changsha, Hunan, China. heiming_yan@yahoo.com.cn
authors.forename: Mingyan
authors.initials: M
authors.lastname: Hei
authors.forename: Yali
authors.initials: Y
authors.lastname: Luo
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Zhang
authors.forename: Furong
authors.initials: F
authors.lastname: Liu
mesh_terms: D045784:Abietanes / Q000494:pharmacology*; D000818:Animals; D000831:Animals, Newborn; D002118:Calcium / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D020925:Hypoxia-Ischemia, Brain / Q000188:drug therapy* / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*
chemical_list: D045784:Abietanes; D004365:Drugs, Chinese Herbal; D018696:Neuroprotective Agents; D016194:Receptors, N-Methyl-D-Aspartate; C021751:tanshinone; D002118:Calcium