title: Electron spin resonance. Part one: a diagnostic method in the biomedical sciences.
abstract: A review is presented of some of the ways in which electron spin resonance (ESR) spectroscopy may be useful to investigate systems of relevance to the biomedical sciences. Specifically considered are: spin-trapping in biological media; the determination of antioxidant efficiencies; lipid-peroxidation; the use of nitroxides as probes of metabolic activity in cells and as structumral probes of cell-membranes; ESR coupled with materials for radiation-dosimetry; food- and drug-irradiation; studies of enzyme systems and ofcyclodextrins; diagnosis of cancer and rheumatoid arthritis; measurement of oxidative stress in synovial tissue in preparation for joint replacement; determination of oxidative species during kidney dialysis; measurement of biological oxygen concentrations (oximetry); trapping in living cells of the endothelium-derived relaxing factor nitric oxide (NO); measurement of hydrogen peroxide; determination of drugs of abuse (opiates); ESR measurements of whole blood and as a means to determine the age of bloodstains for forensic analysis are surveyed, and also a determination of the aqueous volume of human sperm cells is described, among other topics.
authors.affiliation: University of Reading. cjrhodes@fresh-lands.com
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Rhodes
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000032:analysis; D001774:Blood Chemical Analysis; D016503:Drug Delivery Systems; D004578:Electron Spin Resonance Spectroscopy / Q000295:instrumentation / Q000379:methods*; D005514:Food Irradiation; D005609:Free Radicals / Q000032:analysis; D006801:Humans; D015227:Lipid Peroxidation; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D016877:Oxidants / Q000737:chemistry; D010092:Oximetry / Q000379:methods; D011874:Radiometry / Q000379:methods; D013094:Spermatozoa / Q000737:chemistry; D013113:Spin Labels; D019097:Spin Trapping / Q000379:methods
chemical_list: D000975:Antioxidants; D005609:Free Radicals; D016877:Oxidants; D013113:Spin Labels