title: Fasciola hepatica: time-dependent disruption of spermatogenesis following in vivo treatment with triclabendazole.
abstract: Sheep infected with the Cullompton isolate of Fasciola hepatica were treated with triclabendazole at a concentration of 10 mg/kg at 12 weeks post-infection. Adult flukes were recovered from the liver and, where present, from the gall bladder at 48, 72 and 96 h post-treatment (pt). Gross changes to the spermatogenic cells of the testis were examined by histology and ultrastructural alterations were visualised via transmission electron microscopy. Disruption was progressive in nature, with the testis tubules becoming shrunken, vacuolated and gradually more denuded of cellular content over the 96-h time period. From 48 h pt, the number of primary and secondary spermatogonia decreased and multinucleate spermatogonial cells were frequent. Later, developmental stages were uncommon, giving rise to much empty space within the tubules. By 72 h pt, the tubules contained many apoptotic and degraded cells and had an extremely disorganised appearance. At 96 h pt, the tubules were almost completely empty, with the exception of the remains of degraded spermatogenic cells. These results indicate that triclabendazole severely disrupts spermatogenesis in the liver fluke from 48 h pt in vivo.
authors.affiliation: Parasite Therapeutics Research Group, School of Biological Sciences, Medical Biology Centre, The Queen's University of Belfast, 97 Lisburn Road, Belfast, BT9 7BL, UK.
authors.forename: Emma
authors.initials: E
authors.lastname: Toner
authors.forename: Gerard P
authors.initials: GP
authors.lastname: Brennan
authors.forename: Robert E B
authors.initials: RE
authors.lastname: Hanna
authors.forename: Hillary W J
authors.initials: HW
authors.lastname: Edgar
authors.forename: Ian
authors.initials: I
authors.lastname: Fairweather
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology / Q000627:therapeutic use; D001562:Benzimidazoles / Q000494:pharmacology* / Q000627:therapeutic use; D005210:Fasciola hepatica / Q000187:drug effects / Q000648:ultrastructure*; D005211:Fascioliasis / Q000188:drug therapy* / Q000469:parasitology / Q000662:veterinary; D005704:Gallbladder / Q000469:parasitology; D008099:Liver / Q000469:parasitology; D008297:Male; D046529:Microscopy, Electron, Transmission; D012756:Sheep; D012757:Sheep Diseases / Q000188:drug therapy* / Q000469:parasitology; D034561:Sheep, Domestic; D013091:Spermatogenesis / Q000187:drug effects*; D013093:Spermatogonia / Q000187:drug effects / Q000648:ultrastructure*; D013737:Testis / Q000187:drug effects / Q000648:ultrastructure*; D000077682:Triclabendazole
chemical_list: D000871:Anthelmintics; D001562:Benzimidazoles; D000077682:Triclabendazole