title: Identification of dendritic cells in the blood and synovial fluid of children with Juvenile Idiopathic Arthritis.
abstract: Childhood chronic arthritis of unknown etiology is known collectively as juvenile idiopathic arthritis (JIA) and consists of heterogeneous subtypes with unique clinical patterns of disease. JIA is the commonest rheumatic disease in children and may still result in significant disability, with joint deformity, growth impairment, and persistence of active arthritis into adulthood. Basic research is rather focused on rheumatoid arthritis, and this lead to small number of publications considering JIA. In this study we examine, by flow cytometry, the expression of dendritic cells (DCs) in the peripheral blood and synovial fluid of children with active JIA in a group of 220 patients. We reveal a significant decrease in the percentage of immature DCs in the blood of patients compared to control children. Surprisingly, we found higher percentages of mature circulating dendritic cells. Both populations of DCs, immature and mature, were accumulated in patients' synovial fluid. We also confirmed the presence of CD206+/CD209+ in JIA samples, which can represent a population of macrophages with dendritic cells morphology. Our results support the thesis that dendritic cells are crucial in the induction and maintenance of autoimmune response and local inflammation during juvenile idiopathic arthritis.
authors.affiliation: Department of Clinical Immunology, Medical University of Lublin, Poland. jacek.tabarkiewicz@gmail.com
authors.forename: Jacek
authors.initials: J
authors.lastname: Tabarkiewicz
authors.forename: Jacek
authors.initials: J
authors.lastname: Postępski
authors.forename: Edyta
authors.initials: E
authors.lastname: Olesińska
authors.forename: Jacek
authors.initials: J
authors.lastname: Roliński
authors.forename: Ewa
authors.initials: E
authors.lastname: Tuszkiewicz-Misztal
mesh_terms: D000293:Adolescent; D001171:Arthritis, Juvenile / Q000097:blood* / Q000276:immunology* / Q000473:pathology; D015815:Cell Adhesion Molecules / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D003713:Dendritic Cells / Q000276:immunology* / Q000473:pathology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007223:Infant; D037181:Lectins, C-Type / Q000276:immunology; D008297:Male; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins / Q000276:immunology; D011956:Receptors, Cell Surface / Q000276:immunology; D013582:Synovial Fluid / Q000276:immunology*; D055815:Young Adult; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
chemical_list: D015815:Cell Adhesion Molecules; D037181:Lectins, C-Type; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D011956:Receptors, Cell Surface; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin