title: A novel agonistic anti-human death receptor 5 monoclonal antibody with tumoricidal activity induces caspase- and mitochondrial-dependent apoptosis in human leukemia Jurkat cells.
abstract: An agonistic antibody against TNF-related apoptosis-inducing ligand death receptor 5 (DR5) is a practicable candidate drug for antitumor therapy. In this study, a novel murine anti-human DR5 monoclonal antibody, mDRA-6(IgG1-κ), has been generated. This study aimed to explore the caspase-dependent and mitochondrial mechanisms of mDRA-6 in inducing apoptosis in human leukemia Jurkat cells. The apoptotic effects of mDRA-6 on Jurkat cells, which express DR5 on the cell surface, were detected by flow cytometry and western blot after exposure to different doses of mDRA-6 and at fixed doses of mDRA-6 at different times. It was demonstrated that mDRA-6 can induce Jurkat cell apoptosis via caspase- and mitochondrial-dependent pathways. These results indicate that the novel antibody mDRA-6 against DR5 has an antitumor function and may provide a new reagent for tumor therapy.
authors.affiliation: Institute for Immunology of Henan University , Kaifeng, Henan, China.
authors.forename: Yao-Wu
authors.initials: YW
authors.lastname: Du
authors.forename: Ju-Gao
authors.initials: JG
authors.lastname: Chen
authors.forename: Hui-Ling
authors.initials: HL
authors.lastname: Bai
authors.forename: Hong-Ying
authors.initials: HY
authors.lastname: Huang
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Shu-Lian
authors.initials: SL
authors.lastname: Li
authors.forename: Guang-Chao
authors.initials: GC
authors.lastname: Liu
authors.forename: Qi
authors.initials: Q
authors.lastname: Jiang
authors.forename: Jing
authors.initials: J
authors.lastname: Chai
authors.forename: Yue-Ping
authors.initials: YP
authors.lastname: Zhao
authors.forename: Yuan-Fang
authors.initials: YF
authors.lastname: Ma
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D020169:Caspases / Q000096:biosynthesis*; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D004790:Enzyme Induction / Q000187:drug effects; D006801:Humans; D019169:Jurkat Cells; D007938:Leukemia / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D008928:Mitochondria / Q000187:drug effects* / Q000502:physiology; D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand / Q000819:agonists* / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand; D020169:Caspases