title: [Cultivated oral epithelial cells in oral cavity tissue losses treatment with autologous transplantation--case reports].
abstract: The rapid development of bioengineering in recent years enables to search for new therapies involving the use of tissue cultures. The aim of this paper is to study and apply the method of keratinocytes culture of mucous membrane on amniotic membrane to cover the losses in the oral cavity.
  
  
  METHODS
  In four cases keratinocytes culture on amniotic membrane was applied and transplanted to cover antro-oral fistulas and mucosal and mandibular bone losses.
  
  
  RESULTS
  Histopathological and immunohistochemistrical examinations of the culture were performed. Cytokeratin 14, protein 63, conexin 43 and IgG were indicated.
  
  
  CONCLUSIONS
  Autologous transplants of epithelial cells on amniotic membrane are a new effective method to cover unhealed tissue losses in oral cavity with the use of modern methods of tissue engineering.
authors.affiliation: Katedra i Klinika Chirurgii Czaszkowo-Szczekowo-Twarzowej SUM w Katowicach. boguslawa.wylegala@gmail.com
authors.forename: Bogusława
authors.initials: B
authors.lastname: Orzechowska-Wylegała'
authors.forename: Dariusz
authors.initials: D
authors.lastname: Dobrowolski
authors.forename: Bogumił
authors.initials: B
authors.lastname: Wowra
authors.forename: Ewa
authors.initials: E
authors.lastname: Obuchowicz
authors.forename: Anna
authors.initials: A
authors.lastname: Bielecka
mesh_terms: D000328:Adult; D000650:Amnion; D002478:Cells, Cultured; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000637:transplantation*; D008297:Male; D008334:Mandible / Q000601:surgery*; D009061:Mouth Mucosa / Q000473:pathology / Q000601:surgery*; D009957:Oroantral Fistula / Q000601:surgery*; D023822:Tissue Engineering / Q000379:methods*