title: MicroRNA profiling reveals that miR-21, miR486 and miR-214 are upregulated and involved in cell survival in Sézary syndrome.
abstract: Sézary syndrome (SS) is an incurable leukemic variant of cutaneous T-cell lymphoma and its pathogenesis is still unknown. Diagnosis/prognosis may strongly ameliorate the management of SS individuals. Here, we profiled the expression of 470 microRNAs (miRNAs) in a cohort of 22 SS patients, and we identified 45 miRNAs differentially expressed between SS and controls. Using predictive analysis, a list of 19 miRNAs, including miR-21, miR-214, miR-486, miR-18a, miR-342, miR-31 and let-7 members were also found. Moreover, we defined a signature of 14 miRNAs including again miR-21, potentially able to discriminate patients with unfavorable and favorable outcome. We validated our data for miR-21, miR-214 and miR-486 by qRT-PCR, including an additional set of array-independent SS cases. In addition, we also provide an in vitro evidence for a contribution of miR-214, miR-486 and miR-21 to apoptotic resistance of CTCL cell line.
authors.affiliation: Istituto Dermopatico dell'Immacolata-IRCCS, Roma, Italy.
authors.forename: M G
authors.initials: MG
authors.lastname: Narducci
authors.forename: D
authors.initials: D
authors.lastname: Arcelli
authors.forename: M C
authors.initials: MC
authors.lastname: Picchio
authors.forename: C
authors.initials: C
authors.lastname: Lazzeri
authors.forename: E
authors.initials: E
authors.lastname: Pagani
authors.forename: F
authors.initials: F
authors.lastname: Sampogna
authors.forename: E
authors.initials: E
authors.lastname: Scala
authors.forename: P
authors.initials: P
authors.lastname: Fadda
authors.forename: C
authors.initials: C
authors.lastname: Cristofoletti
authors.forename: A
authors.initials: A
authors.lastname: Facchiano
authors.forename: M
authors.initials: M
authors.lastname: Frontani
authors.forename: A
authors.initials: A
authors.lastname: Monopoli
authors.forename: M
authors.initials: M
authors.lastname: Ferracin
authors.forename: M
authors.initials: M
authors.lastname: Negrini
authors.forename: G A
authors.initials: GA
authors.lastname: Lombardo
authors.forename: E
authors.initials: E
authors.lastname: Caprini
authors.forename: G
authors.initials: G
authors.lastname: Russo
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis / Q000235:genetics; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D009707:Nucleosomes / Q000378:metabolism; D012751:Sezary Syndrome / Q000235:genetics* / Q000378:metabolism / Q000401:mortality; D012878:Skin Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000401:mortality; D013601:T-Lymphocytes / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; C524940:MIRN21 microRNA, human; C547397:MIRN214 microRNA, human; C560945:MIRN486 microRNA, human; D035683:MicroRNAs; D009707:Nucleosomes