title: Enantioselective open-tubular capillary electrochromatography using cyclodextrin-modified gold nanoparticles as stationary phase.
abstract: Enantioselective open-tubular CEC (OTCEC) with thiolated β-CD modified gold nanoparticles (CD-GNPs) as stationary phase was developed. The enantioselective OT capillary column was fabricated by electrostatic assembly of poly(diallydimethylammonium chloride) (PDDA) followed by self-adsorption of negatively charged CD-GNPs. The enantioselective capillary column has a steady EOF mobility over a wide pH range of 3.0 to 9.2 (RSD 4.8%), and is quite stable over 240 min with very good column to column reproducibility. Efficient enantioseparation of the presented method was demonstrated by analyzing three drug enantiomers. Our results show that the column exhibits good run-to-run repeatability for enantioseparations and can maintain the enantioselectivity for more than 1 month if the column was stored in CD-GNPs solution at 4 °C.
authors.affiliation: Faculty of Chemistry, Northeast Normal University, Changchun, Jilin, China.
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.forename: Xi
authors.initials: X
authors.lastname: Liu
authors.forename: Fengyi
authors.initials: F
authors.lastname: Jiang
authors.forename: Liping
authors.initials: L
authors.lastname: Guo
authors.forename: Li
authors.initials: L
authors.lastname: Yang
mesh_terms: D053801:Capillary Electrochromatography / Q000379:methods*; D017743:Equipment Reuse; D006046:Gold / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D053768:Metal Nanoparticles / Q000737:chemistry*; D004364:Pharmaceutical Preparations / Q000737:chemistry / Q000302:isolation & purification; D011095:Polyethylenes / Q000737:chemistry; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D015203:Reproducibility of Results; D013237:Stereoisomerism; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D004364:Pharmaceutical Preparations; D011095:Polyethylenes; D000644:Quaternary Ammonium Compounds; D047392:beta-Cyclodextrins; C041004:poly-N,N-dimethyl-N,N-diallylammonium chloride; D006046:Gold