title: Coupling of gelatin to inner surfaces of pore walls in spongy alginate-based scaffolds facilitates the adhesion, growth and differentiation of human bone marrow mesenchymal stromal cells.
abstract: We have developed a novel wide-pore scaffold for cell 3D culturing, based on the technology of freeze-drying of Ca-alginate and gelatin. Two different preparation methodologies were compared: (i) freeze-drying of Na-alginate + gelatin mixed solution followed by the incubation of dried polymer in saturated ethanolic solution of CaCl₂; (ii) freeze-drying of the Na-alginate solution followed by the chemical "activation" of polysaccharide core with divinylsulfone with subsequent gelatin covalent attachment to the inner surfaces of pore walls. The scaffolds produced using the first approach did not provide adhesion and proliferation of human bone marrow mesenchymal stromal cells (MSCs). Conversely, the second approach allowed to obtain scaffolds with a high adherence ability for the cells. When cultured within the latter type of scaffold, MSCs proliferated and were able to differentiate into adipogenic, osteogenic and chondrogenic cell lineages, in response to specific induction stimuli. The results indicate that Ca-alginate wide-pore scaffolds with covalently attached gelatin could be useful for stem cell-based bone, cartilage and adipose tissue engineering.
authors.affiliation: Institute for Problems of Cryobiology and Cryomedicine NAS Ukraine, 23 Peryaslavskaya Str, 61015 Kharkov, Ukraine. yuripetrenko@cryo.org.ua
authors.forename: Yu A
authors.initials: YA
authors.lastname: Petrenko
authors.forename: R V
authors.initials: RV
authors.lastname: Ivanov
authors.forename: A Yu
authors.initials: AY
authors.lastname: Petrenko
authors.forename: V I
authors.initials: VI
authors.lastname: Lozinsky
mesh_terms: D000328:Adult; D000464:Alginates / Q000737:chemistry* / Q000494:pharmacology; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000502:physiology; D002448:Cell Adhesion / Q000187:drug effects; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology; D004548:Elasticity / Q000187:drug effects / Q000502:physiology; D005780:Gelatin / Q000737:chemistry / Q000494:pharmacology*; D020723:Glucuronic Acid / Q000737:chemistry / Q000494:pharmacology; D006603:Hexuronic Acids / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000187:drug effects / Q000502:physiology; D016062:Porosity / Q000187:drug effects; D017154:Stromal Cells / Q000187:drug effects* / Q000502:physiology; D013499:Surface Properties; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D000464:Alginates; D020099:Coated Materials, Biocompatible; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D005780:Gelatin