title: RNA interference of caveolin-1 via lentiviral vector inhibits growth of hypopharyngeal squamous cell carcinoma FaDu cells In Vitro and In Vivo.
abstract: OBJECTIVE
  To investigate the effects of caveolin-1 (CAV1) on the growth of hypopharyngeal squamous cell carcinoma (HSCC) FaDu cells in vitro and in vivo.
  
  
  METHODS
  A CAV1-RNAi-lentivirus construct was transfected into FaDu cells and expression of caveolin-1 was tested by RT-PCR and western blotting analysis. Cell apoptosis was analyzed by transferase-medisated dUTP nick-end labeling (TUNEL) assay. Tumor inhibition effects were investigated by injecting rCAV1-RNAi-lentivirus construct into tumors created with FaDu cells in the HSCC mouse model, with the empty-vector lentivirus as a control. CAV1 expression in xenografts was tested by RT-PCR and immunohistochemistry.
  
  
  RESULTS
  RT-PCR and western blot analysis demonstrated successful construction of the CAV1-RNAi-lentivirus construct producing small hairpin RNA. The average weights and volumes of tumor in mice treated with CAV1-RNAi-lentivirus were lower than in mice with control treatment (P < 0.05). RT-PCR revealed weak positive expression of CAV1 in CAV1-construct-treated xenografts and immunohistochemistry confirmed lower CAV1 expression than in controls.(P < 0.05). In addition, downregulation of CAV1 increased cell apoptosis in vitro.
  
  
  CONCLUSIONS
  The growth of HSCCs could be inhibited by recombinant CAV1-RNAi-lentivirus in vitro and in vivo.
authors.affiliation: Department of Otolaryngology, Qilu Hospital, Shandong University, Jinan, PR China.
authors.forename: Xuening
authors.initials: X
authors.lastname: Zhao
authors.forename: Chao
authors.initials: C
authors.lastname: Ma
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Cai
authors.forename: Dapeng
authors.initials: D
authors.lastname: Lei
authors.forename: Dayu
authors.initials: D
authors.lastname: Liu
authors.forename: Fenglei
authors.initials: F
authors.lastname: Xu
authors.forename: Tong
authors.initials: T
authors.lastname: Jin
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.forename: Xinliang
authors.initials: X
authors.lastname: Pan
mesh_terms: D000818:Animals; D017209:Apoptosis; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology / Q000517:prevention & control*; D051242:Caveolin 1 / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D049109:Cell Proliferation; D005822:Genetic Vectors / Q000008:administration & dosage*; D006801:Humans; D007012:Hypopharyngeal Neoplasms / Q000235:genetics / Q000473:pathology / Q000517:prevention & control*; D007124:Immunoenzyme Techniques; D066298:In Vitro Techniques; D016086:Lentivirus / Q000235:genetics*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
chemical_list: D051242:Caveolin 1; D012333:RNA, Messenger; D034741:RNA, Small Interfering