title: Glycerol homeostasis and metabolism in glycerol kinase carrier mice.
abstract: To examine glycerol homeostasis and metabolism is essential for understanding of pathogenesis and evaluation of treatment efficacy in disorders of glycerol metabolism. In this study, we designed the intraperitoneal glycerol tolerance test (IPGlyTT) and studied glycerol tolerance in vivo using glycerol kinase (Gyk) carrier (C) and wild type (WT) mice. Serum glycerol concentrations in WT almost normalized at 90 min after injection, whereas Gyk C mice retained high serum glycerol concentrations at least until 180 min after injection. These results showed that glycerol tolerance was impaired in Gyk C mice compared to WT mice. The IPGlyTT is useful in accessing glycerol homeostasis and metabolism in animal models such as Gyk C mice and will be valuable in assessing therapeutic interventions in Gyk KO mice.
authors.affiliation: Department of Pediatrics, 22-412 MDCC, David Geffen School of Medicine at UCLA, 10833 Le Conte Avenue, Los Angeles, CA 90095-1752, USA.
authors.forename: Motomichi
authors.initials: M
authors.lastname: Kosuga
authors.forename: Nicole K
authors.initials: NK
authors.lastname: Henderson-MacLennan
authors.forename: Yao-Hua
authors.initials: YH
authors.lastname: Zhang
authors.forename: Bing-Ling
authors.initials: BL
authors.lastname: Huang
authors.forename: Katrina M
authors.initials: KM
authors.lastname: Dipple
authors.forename: Edward R B
authors.initials: ER
authors.lastname: McCabe
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D005990:Glycerol / Q000097:blood / Q000378:metabolism*; D005991:Glycerol Kinase / Q000235:genetics* / Q000378:metabolism*; D006579:Heterozygote; D006706:Homeostasis; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout
chemical_list: D005991:Glycerol Kinase; D005990:Glycerol