title: Polydopamine/liposome coatings and their interaction with myoblast cells.
abstract: Surface-mediated drug delivery is a recent concept, where active surface coatings are employed to deliver therapeutic cargo to cells. Herein, we explore the potential of liposomes embedded in polydopamine (PDA) coatings to serve as drug deposits stored on planar substrates. We quantify the PDA growth rate on glass by XPS and show that PDA coatings support myoblast adherence and proliferation. Further, PDA capping layers were deposited on glass substrates precoated with poly(L-lysine) and zwitterionic liposomes. Already thin PDA capping layers render liposome coated surfaces cell adhesive. We experimentally show for the first time, the internalization of a model hydrophobic cargo, that is, fluorescent lipids embedded within the lipid bilayer of liposomes by the cells from the surface. This is evident from the fluorescence exhibited by the cells grown on PDA coatings containing fluorescently labeled liposomes, with the highest fluorescent intensity found in the close proximity of the cell nuclei. The cargo uptake efficiency depends on the thickness of the PDA capping layer and the cell residence time on the coated substrates. Taken together, we demonstrate the first step toward the establishment of a versatile approach using liposomal drug deposits in polymer thin films for surface-mediated drug delivery.
authors.affiliation: iNANO Interdisciplinary Nanoscience Centre, Aarhus University, Denmark.
authors.forename: Martin E
authors.initials: ME
authors.lastname: Lynge
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Ogaki
authors.forename: Anja Overgård
authors.initials: AO
authors.lastname: Laursen
authors.forename: Jette
authors.initials: J
authors.lastname: Lovmand
authors.forename: Duncan S
authors.initials: DS
authors.lastname: Sutherland
authors.forename: Brigitte
authors.initials: B
authors.lastname: Städler
mesh_terms: D000818:Animals; D002460:Cell Line; D004298:Dopamine / Q000008:administration & dosage / Q000737:chemistry*; D016503:Drug Delivery Systems; D005434:Flow Cytometry; D008081:Liposomes / Q000008:administration & dosage / Q000737:chemistry*; D051379:Mice; D032446:Myoblasts / Q000378:metabolism*; D056951:Photoelectron Spectroscopy; D011108:Polymers / Q000737:chemistry*
chemical_list: D008081:Liposomes; D011108:Polymers; D004298:Dopamine