title: Cellular analysis of the mode of action of methyl-3,5-diiodo-4-(4'-methoxyphenoxy) benzoate (DIME) on tumor cells.
abstract: The hormonally inactive methyl-3,5-diiodo-4-(4'-methoxyphenoxy) benzoate (DIME) at 1-4 mu M concentration induces morphologic changes in E-ras 20 and MDA-MB-231 and other human cancer cells such as multinucleation and enlargement and arrests the cell cycle in the M phase without affecting interphase. Time-lapse videomicroscopy allowed us to follow individual cells. Cells exposed to DIME divided in an abnormal manner, leading to 20% cell fusion and multinucleation. Chromosome painting demonstrated a large accumulation of chromosomes after 5 days of treatment with DIME, consistent with the failure of cells to divide normally. Chromosome breakage was not observed. On the other hand, highly abnormal tubulin-containing structures ensue upon exposure to DIME (1 mu M for 18 h) treatment, indicating an early biochemical action of DIME on the spindle assembly system.
authors.affiliation: SAN FRANCISCO STATE UNIV,ROMBERG TIBURON CTR,OCTAMER RES FDN,ENVIRONM TOXICOL & CHEM RES LAB,TIBURON,CA 94920. UNIV CALIF SAN FRANCISCO,DIV MOL CYTOMETRY,SAN FRANCISCO,CA 94143. UNIV CALIF DAVIS,MED CTR,SCH MED,DEPT PATHOL,SACRAMENTO,CA 95187.
authors.forename: J
authors.initials: J
authors.lastname: Zhen
authors.forename: B
authors.initials: B
authors.lastname: Feuerstein
authors.forename: C
authors.initials: C
authors.lastname: Vidair
authors.forename: W
authors.initials: W
authors.lastname: Hyun
authors.forename: L
authors.initials: L
authors.lastname: Young
authors.forename: E
authors.initials: E
authors.lastname: Kirsten
authors.forename: E
authors.initials: E
authors.lastname: Kun