title: The first intron of human h-ras is regulated by p53 - mediation of specific activation by a p53-binding element.
abstract: We conducted transient reporter gene expression assays to show that the wild-type but not the mutant-type p53 protein positively regulates the expression of the human H-ras gene. This activation of expression is mediated through a specific p53-binding DNA element located in the first intron of the H-ras gene. This element is similar to a previously identified p53-binding element. Without this element, wild-type p53 represses the H-ras promoter, as do several p53 mutants. The repression function is lost when the N-terminal 81 amino acids of the p53 protein are deleted, suggesting that the N-terminus is required for repression. Therefore, p53 seems to regulate the H-ras through both positive and negative mechanisms.
authors.affiliation: UNIV TEXAS,MD ANDERSON CANCER CTR,DEPT NEUROONCOL,HOUSTON,TX 77030. UNIV TEXAS,SW MED CTR,DEPT CELL BIOL & NEUROSCI,DALLAS,TX 75235.
authors.forename: W
authors.initials: W
authors.lastname: Zhang
authors.forename: G
authors.initials: G
authors.lastname: Randhawa
authors.forename: J
authors.initials: J
authors.lastname: Gau
authors.forename: J
authors.initials: J
authors.lastname: Shay
authors.forename: A
authors.initials: A
authors.lastname: Deisseroth