title: Subtractive hybridization analysis of gastric diseases-associated Helicobacter pylori identifies peptidyl-prolyl isomerase as a potential marker for gastric cancer.
abstract: Helicobacter pylori, a microaerophilic Gram-negative bacterium, is known to cause chronic gastritis, peptic ulcer and gastric cancer. Genes that are present in certain isolates may determine strain-specific traits such as disease association and drug resistance. In order to understand the pathogenic mechanisms of gastric diseases, identify molecular markers of the diseases associated with H. pylori strains and provide clues for target treatment of H. pylori-related diseases, a subtracted DNA library was constructed from a gastric cancer-associated H. pylori strain and a superficial gastritis-associated H. pylori strain by suppression subtractive hybridization. The presence of gastric cancer-specific genes was identified by dot blot hybridization, DNA sequencing and PCR-based screening. Twelve gastric cancer-specific high-copy genes and nine low-copy genes were found in gastric cancer compared with the superficial gastritis strain. These genes were confirmed by PCR analysis of H. pylori isolates. Notably, peptidyl-prolyl cis-trans isomerase (PPIase) was detected positively in 11 out of 22 (50%) gastric cancer-associated H. pylori strains. In contrast, <24% of the H. pylori strains from superficial gastritis showed positive results. Given the potential role of PPIases in cell growth, apoptosis and oncogenic transformation, our results suggest that PPIase may represent a novel marker and potential therapeutic target for gastric cancer.
authors.affiliation: Cancer Research Institute and General Surgery, The First Affiliated Hospital, China Medical University, Shenyang, China Mary Babb Randolph Cancer Center, West Virginia University, Morgantown, USA.
authors.forename: Yue-Hua
authors.initials: YH
authors.lastname: Gong
authors.forename: Moye
authors.initials: M
authors.lastname: Chen
authors.forename: Ying
authors.initials: Y
authors.lastname: Xu
authors.forename: Nannan
authors.initials: N
authors.lastname: Dong
authors.forename: Zhikun
authors.initials: Z
authors.lastname: Sang
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yuan
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D016009:Chi-Square Distribution; D004269:DNA, Bacterial / Q000235:genetics; D005756:Gastritis / Q000382:microbiology; D015723:Gene Library; D005819:Genetic Markers / Q000235:genetics; D016480:Helicobacter pylori / Q000201:enzymology* / Q000235:genetics*; D006801:Humans; D015151:Immunoblotting; D009693:Nucleic Acid Hybridization / Q000379:methods; D019696:Peptidylprolyl Isomerase / Q000235:genetics*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013274:Stomach Neoplasms / Q000382:microbiology*
chemical_list: D001426:Bacterial Proteins; D014408:Biomarkers, Tumor; D004269:DNA, Bacterial; D005819:Genetic Markers; D019696:Peptidylprolyl Isomerase