title: Overexpression of Rho GDP-dissociation inhibitor alpha predicts poor survival in oral squamous cell carcinoma.
abstract: Oral cancer has emerged as one of the fastest growing malignancies in Taiwan. However, biomarkers that reliably predict clinical outcomes have yet to be identified. This study was aimed to identify tumor-associated proteins that could be prognostic biomarkers for oral cancer. We compared the protein expression between oral squamous cell carcinoma (OSCC) tissues and adjacent non-cancerous matched tissues (NCMTs) by proteomics. We found that Rho GDP-dissociation inhibitor alpha (RhoGDIα) was differentially expressed in frozen cancerous samples and OSCC cell lines but not in NCMTs. Furthermore, our results indicated that RhoGDIα was selectively upregulated in 78 OSCC tissue sections (p<0.001), and this high expression was significantly correlated with increased tumor size (p<0.05) and poor overall survival (p<0.01). There was a trend that RhoGDIα expression was localized in the cytoplasm of cancer cells but was localized in the plasma membrane of NCMTs. Finally, expression of RhoGDIα was validated to be an independent prognostic indicator for overall survival (p<0.01). These results have identified a novel biomarker that may be useful for prediction of poor prognosis in OSCC patients.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Chi-Mei Medical Center, Liouying, Taiwan; School of Dentistry, National Yang-Ming University, Taipei, Taiwan.
authors.forename: Wei-Fan
authors.initials: WF
authors.lastname: Chiang
authors.forename: Hsu-Chueh
authors.initials: HC
authors.lastname: Ho
authors.forename: Hua-Yuan
authors.initials: HY
authors.lastname: Chang
authors.forename: Chien-Chih
authors.initials: CC
authors.lastname: Chiu
authors.forename: Yuh-Ling
authors.initials: YL
authors.lastname: Chen
authors.forename: Tzyh-Chyuan
authors.initials: TC
authors.lastname: Hour
authors.forename: Shu-Ju
authors.initials: SJ
authors.lastname: Chuang
authors.forename: Yu-Jen
authors.initials: YJ
authors.lastname: Wu
authors.forename: Hau-Ren
authors.initials: HR
authors.lastname: Chen
authors.forename: Jen-Hao
authors.initials: JH
authors.lastname: Chen
authors.forename: Shyun-Yeu
authors.initials: SY
authors.lastname: Liu
authors.forename: Chin-Li
authors.initials: CL
authors.lastname: Lu
authors.forename: Jeff Yi-Fu
authors.initials: JY
authors.lastname: Chen
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism* / Q000401:mortality; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D005260:Female; D020730:Guanine Nucleotide Dissociation Inhibitors / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000401:mortality; D011237:Predictive Value of Tests; D011379:Prognosis; D016019:Survival Analysis; D013624:Taiwan / Q000453:epidemiology; D015854:Up-Regulation; D062947:rho-Specific Guanine Nucleotide Dissociation Inhibitors
chemical_list: D014408:Biomarkers, Tumor; D020730:Guanine Nucleotide Dissociation Inhibitors; D062947:rho-Specific Guanine Nucleotide Dissociation Inhibitors