title: Dietary fish oil supplements increase tissue n-3 fatty acid composition and expression of delta-6 desaturase and elongase-2 in Jade Tiger hybrid abalone.
abstract: This study was conducted to investigate the effects of fish oil (FO) supplements on fatty acid composition and the expression of ∆6 desaturase and elongase 2 genes in Jade Tiger abalone. Five test diets were formulated to contain 0.5, 1.0, 1.5, 2.0 and 2.5% of FO respectively, and the control diet was the normal commercial abalone diet with no additional FO supplement. The muscle, gonad and digestive glands (DG) of abalone fed with all of the five test diets showed significantly high levels of total n-3 polyunsaturated fatty acid (PUFA), eicosapentaenoic acid (EPA), docosapentaenoic acid n-3 (DPAn-3), and docosahexaenoic acid (DHA) than the control group. In all three types of tissue, abalone fed diet supplemented with 1.5% FO showed the highest level of these fatty acids (P < 0.05). For DPAn-3 the higher level was also found in muscle and gonad of abalone fed diet supplemented with 2% FO (P < 0.05). Elongase 2 expression was markedly higher in the muscle of abalone fed diet supplemented with 1.5% FO (P < 0.05), followed by the diet containing 2% FO supplement. For ∆6 desaturase, significantly higher expression was observed in muscle of abalone fed with diet containing 0.5% FO supplement (P < 0.05). Supplementation with FO in the normal commercial diet can significantly improve long chain n-3 PUFA level in cultured abalone, with 1.5% being the most effective supplementation level.
authors.affiliation: School of Biomedical and Health Sciences, Victoria University, P.O. Box 14428, MCMC, Melbourne, VIC, 8001, Australia.
authors.forename: Hintsa T
authors.initials: HT
authors.lastname: Mateos
authors.forename: Paul A
authors.initials: PA
authors.lastname: Lewandowski
authors.forename: Xiao Q
authors.initials: XQ
authors.lastname: Su
mesh_terms: D000123:Acetyltransferases / Q000378:metabolism*; D000818:Animals; D004032:Diet; D019587:Dietary Supplements; D000081031:Fatty Acid Elongases; D015525:Fatty Acids, Omega-3 / Q000737:chemistry* / Q000378:metabolism; D005395:Fish Oils / Q000008:administration & dosage* / Q000737:chemistry / Q000378:metabolism; D050602:Linoleoyl-CoA Desaturase / Q000378:metabolism*; D008974:Mollusca / Q000033:anatomy & histology / Q000737:chemistry* / Q000201:enzymology*; D014018:Tissue Distribution
chemical_list: D015525:Fatty Acids, Omega-3; D005395:Fish Oils; D050602:Linoleoyl-CoA Desaturase; D000123:Acetyltransferases; D000081031:Fatty Acid Elongases