title: 17α-Ethinylestradiol and nonylphenol affect the development of forebrain GnRH neurons through an estrogen receptors-dependent pathway.
abstract: There is growing evidence that neuroendocrine circuits controlling development and reproduction are targeted by EDCs. We have previously demonstrated that low concentrations of 17α-ethinylestradiol (EE2) disrupt the development of forebrain GnRH neurons during zebrafish development. The objectives of the present study were to determine whether the weak estrogenic compound, nonylphenol (NP), could elicit similar effects to EE2 and to what extent the estrogen receptors are involved in mediating these effects. Using immunohistochemistry, we confirmed that EE2 exposure induces an increase in the number of GnRH-ir neurons and we demonstrated that NP is able to produce similar effects in a concentration-dependent manner. The effects of both NP and EE2 were shown to be blocked by the estrogen receptors (ERs) antagonist ICI 182-780, demonstrating the involvement of functional ERs in mediating their effects. Altogether, these results highlight the need to consider neuroendocrine networks as critical endpoints in the field of endocrine disruption.
authors.affiliation: Unité d'évaluation des risques écotoxicologiques, Direction des Risques Chroniques, Institut National de l'Environnement Industriel et des Risques, BP 2. F-60550 Verneuil-en-Halatte, France.
authors.forename: Mélanie
authors.initials: M
authors.lastname: Vosges
authors.forename: Olivier
authors.initials: O
authors.lastname: Kah
authors.forename: Nathalie
authors.initials: N
authors.lastname: Hinfray
authors.forename: Edith
authors.initials: E
authors.lastname: Chadili
authors.forename: Yann
authors.initials: Y
authors.lastname: Le Page
authors.forename: Yves
authors.initials: Y
authors.lastname: Combarnous
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Porcher
authors.forename: François
authors.initials: F
authors.lastname: Brion
mesh_terms: D000818:Animals; D001141:Aromatase / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000187:drug effects; D052244:Endocrine Disruptors / Q000633:toxicity; D004958:Estradiol / Q000031:analogs & derivatives / Q000494:pharmacology; D004965:Estrogen Antagonists / Q000494:pharmacology; D004967:Estrogens / Q000633:toxicity*; D004997:Ethinyl Estradiol / Q000633:toxicity*; D000077267:Fulvestrant; D007987:Gonadotropin-Releasing Hormone / Q000502:physiology; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D010636:Phenols / Q000633:toxicity*; D016548:Prosencephalon / Q000502:physiology; D011960:Receptors, Estrogen / Q000037:antagonists & inhibitors / Q000502:physiology*; D015027:Zebrafish
chemical_list: D052244:Endocrine Disruptors; D004965:Estrogen Antagonists; D004967:Estrogens; D010636:Phenols; D011960:Receptors, Estrogen; D000077267:Fulvestrant; D007987:Gonadotropin-Releasing Hormone; D004997:Ethinyl Estradiol; D004958:Estradiol; C025256:nonylphenol; D001141:Aromatase