title: Development of an enzyme-linked immunosorbent assay for determination of pretilachlor in water and soil.
abstract: An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) has been developed for detection of pretilachlor in water and soil. An immunogen was prepared from haptens of pretilachlor conjugated to bovine serum albumin(BSA). The specific polyclonal antibodies were obtained by immunizing New Zealand white rabbits. The influence of parameters including concentrations of methanol, ionic strength and pH values were optimized to improve the sensitivity of the assay. The optimized ELISA was shown to have a high sensitivity and specificity for pretilachlor. Under optimal conditions, the ELISA has demonstrated an 50% inhibitory concentration (IC(50)) value of 0.0359 mg/L with a limit of detection (LOD, IC(10)) of 6.9 ng/L. The cross-reactivities to some analogs of pretilachlor (acetochlor, butachlor, metazachlor and metalaxyl) were below 1.5%. The average recoveries of pretilachlor from distilled water, tap water, paddy water and soil were in the range of 77.0-115.2% between 0.005 and 5.0mg/L. The results of ELISA for spiked samples were confirmed by GC-ECD with a high correlation coefficient of 0.9950(n=11). Thus, the ELISA proven to be a sensitive, specific, inexpensive and quantitative tool for detection of pretilachlor from four kinds of spiked samples.
authors.affiliation: Department of Pesticide Science, College of Plant Protection, Nanjing Agricultural University, Jiangsu Key Laboratory of Pesticide Science, Key Laboratory of Integrated Management of Crop Diseases and Pests (Nanjing Agricultural University), Ministry of Education, Nanjing 210095, PR China.
authors.forename: Zhen-Jiang
authors.initials: ZJ
authors.lastname: Liu
authors.forename: Peng-Min
authors.initials: PM
authors.lastname: Yu
authors.forename: Song
authors.initials: S
authors.lastname: Fang
authors.forename: Jia-Qin
authors.initials: JQ
authors.lastname: Fan
authors.forename: Ming-Hua
authors.initials: MH
authors.lastname: Wang
mesh_terms: D000083:Acetanilides / Q000032:analysis*; D000818:Animals; D000906:Antibodies / Q000032:analysis / Q000276:immunology; D004784:Environmental Monitoring / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005618:Fresh Water / Q000737:chemistry*; D006241:Haptens / Q000032:analysis / Q000276:immunology; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000032:analysis / Q000276:immunology; D012987:Soil / Q000737:chemistry*; D012989:Soil Pollutants / Q000032:analysis*; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D000083:Acetanilides; D000906:Antibodies; D006241:Haptens; D012987:Soil; D012989:Soil Pollutants; D014874:Water Pollutants, Chemical; D012710:Serum Albumin, Bovine; C118050:pretilachlor