title: Differential regulation of diacylglycerol kinase isoform in human failing hearts.
abstract: Evidence from several studies indicates the importance of Gαq protein-coupled receptor (GPCR) signaling pathway, which includes diacylglycerol (DAG), and protein kinase C, in the development of heart failure. DAG kinase (DGK) acts as an endogenous regulator of GPCR signaling pathway by catalyzing and regulating DAG. Expressions of DGK isoforms α, ε, and ζ in rodent hearts have been detected; however, the expression and alteration of DGK isoforms in a failing human heart has not yet been examined. In this study, we detected mRNA expressions of DGK isoforms γ, η, ε, and ζ in failing human heart samples obtained from patients undergoing cardiovascular surgery with cardiopulmonary bypass. Furthermore, we investigated modulation of DGK isoform expression in these hearts. We found that expressions of DGKη and DGKζ were increased and decreased, respectively, whereas those of DGKγ and DGKε remained unchanged. This is the first report that describes the differential regulation of DGK isoforms in normal and failing human hearts.
authors.affiliation: Department of Cardiology, Pulmonology, and Nephrology, Yamagata University School of Medicine, Yamagata, Japan.
authors.forename: Olga
authors.initials: O
authors.lastname: Bilim
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Shishido
authors.forename: Shuji
authors.initials: S
authors.lastname: Toyama
authors.forename: Satoshi
authors.initials: S
authors.lastname: Suzuki
authors.forename: Toshiki
authors.initials: T
authors.lastname: Sasaki
authors.forename: Tatsuro
authors.initials: T
authors.lastname: Kitahara
authors.forename: Mitsuaki
authors.initials: M
authors.lastname: Sadahiro
authors.forename: Yasuchika
authors.initials: Y
authors.lastname: Takeishi
authors.forename: Isao
authors.initials: I
authors.lastname: Kubota
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002306:Cardiac Volume / Q000235:genetics; D019852:Diacylglycerol Kinase / Q000096:biosynthesis / Q000235:genetics*; D005260:Female; D005500:Follow-Up Studies; D005786:Gene Expression Regulation; D006333:Heart Failure / Q000201:enzymology / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009206:Myocardium / Q000201:enzymology; D012313:RNA / Q000235:genetics*; D012189:Retrospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D012313:RNA; D019852:Diacylglycerol Kinase