title: Expression of the membrane-cytoskeletal linker Ezrin in salivary gland adenoid cystic carcinoma.
abstract: OBJECTIVE
  The aim of this study was to investigate the relationship between membrane cytoskeleton linker protein Ezrin and CD44v6, iNOS, Ki-67, and clinicopathologic characteristics, and the prognostic significance of Ezrin expression in salivary gland adenoid cystic carcinoma (SACC).
  
  
  METHODS
  Immunohistochemistry and reverse-transcription polymerase chain reaction were used to quantify the expression of Ezrin, CD44v6, inducible nitric oxide synthase (iNOS), and Ki-67 in 75 primary SACCs, 25 tumor-free salivary tissues, and 2 SACC cell lines (ACC-M and ACC-2). Survival analysis was performed to find the prognostic significance of Ezrin expression.
  
  
  RESULTS
  Expressions of Ezrin, CD44v6, iNOS, and Ki-67 in SACC tissues, especially with distant metastasis, were significantly higher than in tumor-free tissues. Ezrin mRNA and protein levels in ACC-M cells were significantly higher than in ACC-2 cells. Ezrin, CD44v6, iNOS, and Ki-67 expressions were significantly higher in solid pattern than in cribriform and tubular patterns. Ezrin and its partners, CD44v6, iNOS, and Ki-67, were significantly related to tumor size, clinical stage, perineural and vascular invasion, and recurrence. Furthermore, Ezrin had an independent prognostic effect on overall survival.
  
  
  CONCLUSIONS
  The increased expression of Ezrin and its partners, CD44v6, iNOS, and Ki-67, in SACC correlated with histologic patterns, may play a role in distant metastasis, and might indicate poor clinical outcome.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Sun Yat-sen Memorial Hospital No 2 Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China.
authors.forename: You-yuan
authors.initials: YY
authors.lastname: Wang
authors.forename: Wei-liang
authors.initials: WL
authors.lastname: Chen
authors.forename: Zhi-quan
authors.initials: ZQ
authors.lastname: Huang
authors.forename: Zhao-hui
authors.initials: ZH
authors.lastname: Yang
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.forename: Jian-guang
authors.initials: JG
authors.lastname: Wang
authors.forename: Hai-gang
authors.initials: HG
authors.lastname: Li
authors.forename: Jin-Song
authors.initials: JS
authors.lastname: Li
mesh_terms: D003528:Carcinoma, Adenoid Cystic / Q000473:pathology* / Q000556:secondary; D045744:Cell Line, Tumor; D003598:Cytoskeletal Proteins / Q000032:analysis*; D005260:Female; D005500:Follow-Up Studies; D015870:Gene Expression; D006801:Humans; D018960:Hyaluronan Receptors / Q000032:analysis; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000032:analysis; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009364:Neoplasm Recurrence, Local / Q000473:pathology; D009367:Neoplasm Staging; D052247:Nitric Oxide Synthase Type II / Q000032:analysis; D051179:Plakins / Q000032:analysis*; D011379:Prognosis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012468:Salivary Gland Neoplasms / Q000473:pathology*; D012469:Salivary Glands / Q000473:pathology; D015996:Survival Rate; D000099149:Ezrin
chemical_list: D003598:Cytoskeletal Proteins; D018960:Hyaluronan Receptors; D019394:Ki-67 Antigen; D052247:Nitric Oxide Synthase Type II; D051179:Plakins; D000099149:Ezrin; C112697:CD44v6 antigen; C496319:NOS2 protein, human