title: Inhibition of proliferation of rat lens epithelial cell by overexpression of KLF6.
abstract: OBJECTIVE
  Proliferation of lens epithelial cell (LEC) is main principle for posterior capsular opacity (PCO) following surgery. We investigated whether overexpression of Krüppel-like factor 6 (KLF6) can be employed to increase protein 21 (p21) and protein 27 kinase inhibition protein 1 (p27(kip1)) levels and its effect on proliferation of LEC.
  
  
  METHODS
  A plasmid containing KLF6 cDNA was used to increase the level of KLF6 protein in rat lens epithelial cells (rLEC) which can lead to consequent degradation of p21 and p27(kip1). Cell proliferation was assayed by cell counts and bromodeoxyuridine (BrdU) Incorporation.
  
  
  RESULTS
  western blot analysis showed increased levels of KLF6, p21, and p27(kip1) in cells transfected with pKLF6 cDNA. pKLF6 cDNA transfected cells showed less compared with control cells in vitro.
  
  
  CONCLUSIONS
  pKLF6 cDNA inhibited cell proliferation and decreased cell viability of LEC by unregulation of p21 and p27(kip1).
authors.affiliation: Department of Ophthalmology, First Clinic College of Harbin Medical University, Harbin, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Su
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: Dan
authors.initials: D
authors.lastname: Zhou
authors.forename: Weiqi
authors.initials: W
authors.lastname: Gao
authors.forename: Qi
authors.initials: Q
authors.lastname: Hu
authors.forename: Hao
authors.initials: H
authors.lastname: Cui
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D001973:Bromodeoxyuridine / Q000032:analysis; D002386:Cataract / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D002452:Cell Count; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D002470:Cell Survival / Q000235:genetics; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000235:genetics / Q000378:metabolism*; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000235:genetics / Q000378:metabolism*; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D015870:Gene Expression; D000075723:Kruppel-Like Factor 6; D051741:Kruppel-Like Transcription Factors / Q000235:genetics / Q000378:metabolism*; D007908:Lens, Crystalline / Q000378:metabolism / Q000473:pathology; D010957:Plasmids; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D014162:Transfection
chemical_list: C493958:Cdkn1a protein, rat; C493962:Cdkn1b protein, rat; D050759:Cyclin-Dependent Kinase Inhibitor p21; C491357:Klf6 protein, rat; D000075723:Kruppel-Like Factor 6; D051741:Kruppel-Like Transcription Factors; D011518:Proto-Oncogene Proteins; D050760:Cyclin-Dependent Kinase Inhibitor p27; D001973:Bromodeoxyuridine