title: DNA-binding study of anthraquinone derivatives using chemometrics methods.
abstract: This work presents the interaction of two anthraquinones including quinizarin (1, 4-dihydroxyanthraquinone) and danthron (1, 8-dihydroxyanthraquinone) with CT-DNA in a mixture of Brittone-Robinson buffer (pH=7) with 50% of ethanol by UV-vis absorption, circular dichroism spectroscopic methods and viscosity measurements. The PCA and INDICES methods were used for predicting the number of light-absorbing components. Partially intercalative and hydrogen binding were found to be the dominant binding modes between anthraquinones and CT-DNA. The effect of ionic strength and pH on the behavior of the above system and also the interaction of anthraquinones with ds and ss-DNA were used to confirm the mentioned binding modes. The EQUISPEC software and nonlinear least-squares analysis were applied for binding constant determination.
authors.affiliation: Faculty of Chemistry, Sensor and Biosensor Research Center (SBRC) & Nanoscience and Nanotechnology Research Center (NNRC), Razi University, Kermanshah, Iran. mbgholivand@yahoo.com
authors.forename: M B
authors.initials: MB
authors.lastname: Gholivand
authors.forename: S
authors.initials: S
authors.lastname: Kashanian
authors.forename: H
authors.initials: H
authors.lastname: Peyman
authors.forename: H
authors.initials: H
authors.lastname: Roshanfekr
mesh_terms: D000818:Animals; D000880:Anthraquinones / Q000737:chemistry*; D002021:Buffers; D002417:Cattle; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000737:chemistry*; D006860:Hydrogen Bonding; D006863:Hydrogen-Ion Concentration; D007364:Intercalating Agents / Q000737:chemistry*; D007700:Kinetics; D009994:Osmolar Concentration; D013950:Thymus Gland / Q000737:chemistry; D014783:Viscosity
chemical_list: D000880:Anthraquinones; D002021:Buffers; D004277:DNA, Single-Stranded; D007364:Intercalating Agents; C034890:1,4-dihydroxyanthraquinone; D004247:DNA; C004315:danthron