title: Proteomic analysis of RNA interference induced knockdown plant.
abstract: RNA interference (RNAi) is a useful research tool for the specific deletion, or knockdown, of target genes that can be exploited both in cultured plant cells and in whole plants. In RNAi, hairpin RNA (hpRNA)-transduced lines are used to identify loss-of-function mutations in multi-copy genes with redundant functions in polyploid plant species. Plants transformed with hpRNA exhibit a range of phenotypes resulting from complete knockdown to weak suppression or tissue- and stage-specific knockdown. Functional genomics using proteomic analysis with two-dimensional gel electrophoresis (2-DE) and mass spectrometry provides valuable information about altered levels of expression of specific genes in biological samples. Here, we describe the proteomic analysis of Oryza sativa (Os) thioredoxin m (Ostrxm) knockdown using 2-DE and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS).
authors.affiliation: Division of Applied Life Science (BK21 Program) and PMBBRC, Gyeongsang National University, 660-701 Jinju, Korea. sylee@gnu.ac.kr
authors.forename: Sang Yeol
authors.initials: SY
authors.lastname: Lee
authors.forename: Kyun Oh
authors.initials: KO
authors.lastname: Lee
mesh_terms: D055785:Gene Knockdown Techniques; D023061:Gene Order; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D012275:Oryza / Q000235:genetics* / Q000378:metabolism*; D010940:Plant Proteins / Q000302:isolation & purification / Q000378:metabolism; D040901:Proteomics; D034622:RNA Interference; D018749:RNA, Plant / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014170:Transformation, Genetic
chemical_list: D010940:Plant Proteins; D018749:RNA, Plant