title: Monocyte chemoattractant protein-1 modulates invasion and apoptosis of PC-3M prostate cancer cells via regulating expression of VEGF, MMP9 and caspase-3.
abstract: Prostate cancer is a form of malignancy that is most likely to develop in older males, but because of the propensity to metastasize to parts of the body, particularly the bones, can have a deleterious impact on quality of life. Recently monocyte chemoattractant protein-1 (MCP-1) has been shown to play important role in prostate cancer progression and metastasi. In this study we aimed to investigate the mechanisms underlying its functional roles. In vitro transwell invasion assays with PC-3M prostate cancer cells demonstrated MCP-1 promotion of invasion, while annexin V-FITC and TUNEL confirmed inhibition of apoptosis. Treatment MCP-1 further led to significant upregulation of VEGF and MMP-9 and downregulation of Caspase-3 at both mRNA and protein levels compared with untreated control (P < 0.05), while siRNA mediated knockdown reversed these changes. Taken together, our results indicate important roles of MCP-1 in prostate cancer progression and metastasis and our finding of regulation of VEGF, MMP-9 and Caspase-3 expression open up new possibilities for targeted therapy.
authors.affiliation: Fourth Hospital of Jilin University, Changchun, China.
authors.forename: Chao-Ling
authors.initials: CL
authors.lastname: Shi
authors.forename: Chang-Hai
authors.initials: CH
authors.lastname: Yu
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Dan
authors.initials: D
authors.lastname: Zhao
authors.forename: Xi-Hua
authors.initials: XH
authors.lastname: Chang
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Wang
mesh_terms: D017209:Apoptosis; D015153:Blotting, Western; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism*; D002465:Cell Movement; D049109:Cell Proliferation; D018932:Chemokine CCL2 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology*; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism*
chemical_list: C111835:CCL2 protein, human; D018932:Chemokine CCL2; D012333:RNA, Messenger; D034741:RNA, Small Interfering; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D053148:Caspase 3; D020780:Matrix Metalloproteinase 9