title: A direct, efficient method for the preparation of siRNAs containing ribo-like North bicyclo[3.1.0]hexane pseudosugars.
abstract: An efficient method for the preparation of siRNAs modified with ribo-like North bicyclo[3.1.0]hexane pseudosugars is described. The combined use of 2'-O-(2-cyanoethoxymethyl) (CEM) and 2'-O-TBDMS protection was successfully employed for RNA synthesis with the added advantage that both groups were efficiently removed in a single step. The resulting North ribo-methanocarba-modified siRNAs are compatible with the intracellular RNAi machinery and can mediate specific degradation of target mRNA.
authors.affiliation: Institute for Research in Biomedicine (IRB Barcelona), Networking Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Barcelona, Spain.
authors.forename: Montserrat
authors.initials: M
authors.lastname: Terrazas
authors.forename: Anna
authors.initials: A
authors.lastname: Aviñó
authors.forename: Maqbool A
authors.initials: MA
authors.lastname: Siddiqui
authors.forename: Victor E
authors.initials: VE
authors.lastname: Marquez
authors.forename: Ramon
authors.initials: R
authors.lastname: Eritja
mesh_terms: D054329:Carbasugars / Q000737:chemistry*; D015394:Molecular Structure; D009705:Nucleosides / Q000138:chemical synthesis* / Q000737:chemistry; D012333:RNA, Messenger / Q000187:drug effects; D034741:RNA, Small Interfering / Q000138:chemical synthesis* / Q000737:chemistry; D012689:Sequence Homology, Nucleic Acid
chemical_list: D054329:Carbasugars; D009705:Nucleosides; D012333:RNA, Messenger; D034741:RNA, Small Interfering