title: Effects of per- and polyfluorinated compounds on adult rat testicular cells following in vitro exposure.
abstract: Testicular toxicity is observed following exposure of rats to per- and polyfluorinated compounds (PFCs). Such compounds were also shown to induce oxidative stress and changes in ABC efflux transporters e.g. P-gp, implying two mechanisms which may contribute to testicular toxicity. We studied the toxicity of four PFCs (PFOA, PFNA, 8:2 FTOH and 6:2 FTOH) on primary rat testicular cells. DNA damage was studied by the comet assay including Fpg enzyme treatment to detect oxidative lesions. The levels of the ABC efflux transporters Bcrp1, Oat2 and P-gp were studied by real-time RT-PCR or flow cytometry. A PFNA associated increase in DNA SSBs was attributed to a subpopulation of moderately damaged cells possibly associated with cytotoxicity. No significant increase in oxidative DNA damage was measured for any of the PFCs. Expression levels of ABC efflux transporters suggest that PFCs may increase expression levels of the P-gp protein and the Oat2 gene.
authors.affiliation: Norwegian Institute of Public Health, Division of Environmental Medicine, Department of Chemical Toxicology, P.O. Box 4404 Nydalen, NO-0403 Oslo, Norway.
authors.forename: Birgitte
authors.initials: B
authors.lastname: Lindeman
authors.affiliation: Norwegian Institute of Public Health, Division of Environmental Medicine, Department of Chemical Toxicology, P.O. Box 4404 Nydalen, NO-0403 Oslo, Norway.
authors.forename: Christine
authors.initials: C
authors.lastname: Maass
authors.affiliation: Norwegian Institute of Public Health, Division of Environmental Medicine, Department of Chemical Toxicology, P.O. Box 4404 Nydalen, NO-0403 Oslo, Norway.
authors.forename: Nur
authors.initials: N
authors.lastname: Duale
authors.affiliation: Norwegian Institute of Public Health, Division of Environmental Medicine, Department of Chemical Toxicology, P.O. Box 4404 Nydalen, NO-0403 Oslo, Norway.
authors.forename: Kristine B
authors.initials: KB
authors.lastname: Gützkow
authors.affiliation: Norwegian Institute of Public Health, Division of Environmental Medicine, Department of Chemical Toxicology, P.O. Box 4404 Nydalen, NO-0403 Oslo, Norway.
authors.forename: Gunnar
authors.initials: G
authors.lastname: Brunborg
authors.affiliation: Norwegian Institute of Public Health, Division of Environmental Medicine, Department of Chemical Toxicology, P.O. Box 4404 Nydalen, NO-0403 Oslo, Norway. Electronic address: ashild.andreassen@fhi.no.
authors.forename: Åshild
authors.initials: Å
authors.lastname: Andreassen
mesh_terms: D000818:Animals; D002210:Caprylates / Q000633:toxicity; D018929:Cell Culture Techniques; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D020552:Comet Assay; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D005466:Fluorocarbons / Q000633:toxicity*; D015870:Gene Expression / Q000187:drug effects; D008297:Male; D012313:RNA / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D013737:Testis / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism
chemical_list: D002210:Caprylates; D005466:Fluorocarbons; C033729:fluorotelomer alcohols; D012313:RNA; C018959:hexadecafluoro-nonanoic acid; C023036:perfluorooctanoic acid