title: A novel combination of selective agents for isolation of Leptospira species.
abstract: A novel combination of antimicrobial agents (sulfamethoxazole, 40 μg/mL; trimethoprim, 20 μg/mL; amphotericin B, 5 μg/mL; fosfomycin, 400 μg/mL; and 5-fluorouracil, 100 μg/mL) was developed for selective isolation of leptospires from contaminated samples. The growth of 16 microorganisms considered as possible contaminants during isolation of Leptospira were inhibited by this antimicrobial cocktail. In contrast, the growth of a smaller inoculum (10(1) cells per mL) of 25 Leptospira strains (representing 18 serovars/serogroups of 5 species) was not suppressed by this antimicrobial combination. This cocktail, after being incorporated into Leptospira growth medium (Korthof's), successfully detected leptospires in environmental soil and water. Based on the results, this selective medium has the potential to meet the existing need for an effective selective medium for the isolation of Leptospira.
authors.affiliation: Department of Bacteriology, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan. antu_cc@yahoo.com.sg
authors.forename: Antara
authors.initials: A
authors.lastname: Chakraborty
authors.forename: Satoshi
authors.initials: S
authors.lastname: Miyahara
authors.forename: Sharon Y A M
authors.initials: SY
authors.lastname: Villanueva
authors.forename: Mitsumasa
authors.initials: M
authors.lastname: Saito
authors.forename: Nina G
authors.initials: NG
authors.lastname: Gloriani
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Yoshida
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D001431:Bacteriological Techniques / Q000379:methods*; D003470:Culture Media / Q000737:chemistry*; D007919:Leptospira / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification*; D012988:Soil Microbiology; D014871:Water Microbiology
chemical_list: D000890:Anti-Infective Agents; D003470:Culture Media