title: Icariin induces the expression of toll-like receptor 9 in ana-1 murine macrophages.
abstract: Icariin is the major pharmacologically active compound of Herba epimedii which has been used as a tonic, aphrodisiac and an antirheumatic in traditional Chinese medicine. This study analysed the effect of icariin on the expression of Toll-like receptor 9 (TLR9) which plays an important role in regulation of the innate immune response. Stimulation of Ana-1 murine macrophages with icariin induced a significant dose-dependent expression of TLR9, and its mRNA expression which increased from 3 h post-treatment was approximately five-fold that of DMSO-treated cells. Several molecules, such as myeloid differentiation factor 88, tumor necrosis factor-α and interleukin 6, which are involved in the TLR9 downstream signaling pathway, were also significantly up-regulated in response to icariin stimulation. Our findings demonstrated that icariin is able to induce the expression of TLR9.
authors.affiliation: Guangxi Botanic Garden of Medicinal Plants, No. 189, Changgang Road, Nanning 530023, PR China. liboshi1963@vip.163.com
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Lina
authors.initials: L
authors.lastname: Peng
authors.forename: Jianhua
authors.initials: J
authors.lastname: Miao
authors.forename: Yafeng
authors.initials: Y
authors.lastname: Qiu
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhou
authors.forename: Xiaoqi
authors.initials: X
authors.lastname: Gao
authors.forename: Yongli
authors.initials: Y
authors.lastname: Xu
authors.forename: Zixue
authors.initials: Z
authors.lastname: Shi
authors.forename: Donghua
authors.initials: D
authors.lastname: Shao
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Ma
mesh_terms: D000818:Animals; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005419:Flavonoids / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D015850:Interleukin-6 / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D053594:Myeloid Differentiation Factor 88 / Q000378:metabolism; D051217:Toll-Like Receptor 9 / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D015850:Interleukin-6; C507780:Myd88 protein, mouse; D053594:Myeloid Differentiation Factor 88; C495370:Tlr9 protein, mouse; D051217:Toll-Like Receptor 9; D014409:Tumor Necrosis Factor-alpha; C056599:icariin