title: Influence of pre-analytical factors on α-galactosidase A, arylsulfatase B and α-glucosidase activities measured on dried blood spots on filter paper.
abstract: OBJECTIVE
  To analyze the effect of blood collection and storage conditions on activity of α-galactosidase A, arylsulfatase B and α-glucosidase.
  
  
  METHODS
  Blood was collected in EDTA, heparin, or direct spotting on filter paper and stored at different temperatures (-20, 4, 25 and 37°C) and storage times (3, 10, 17 and 180 days). The influence of filter paper size was also assessed (3.0 and 1.2mm).
  
  
  RESULTS
  No statistically significant difference was observed between the three collection methods. α-Glucosidase A activity significantly decreased after the 10th day, while arylsulfatase B activity only differed significantly after the 180th day, and α-galactosidase A activity remained constant throughout this storage time. Excellent correlation coefficients were observed for the two filter paper sizes used.
  
  
  CONCLUSIONS
  Both paper sizes may be employed. Filter paper specimens should be transported under refrigeration as soon as possible after blood collection.
authors.affiliation: Department and Program of Post-graduation in Biochemistry, Federal University of Rio Grande do Sul, UFRGS, Porto Alegre, RS, Brazil.
authors.forename: Cristina D
authors.initials: CD
authors.lastname: de Castilhos
authors.forename: Jamila
authors.initials: J
authors.lastname: Mezzalira
authors.forename: Mariana P S
authors.initials: MP
authors.lastname: Goldim
authors.forename: Janice C
authors.initials: JC
authors.lastname: Coelho
mesh_terms: D001800:Blood Specimen Collection / Q000379:methods*; D005374:Filtration; D006801:Humans; D020051:N-Acetylgalactosamine-4-Sulfatase / Q000097:blood*; D010209:Paper; D013696:Temperature; D013997:Time Factors; D000519:alpha-Galactosidase / Q000097:blood*; D000520:alpha-Glucosidases / Q000097:blood*
chemical_list: D020051:N-Acetylgalactosamine-4-Sulfatase; D000520:alpha-Glucosidases; D000519:alpha-Galactosidase