title: In vitro activity of new antimicrobial agents against glycopeptide-resistant Enterococcus faecium clinical isolates from France between 2006 and 2008.
abstract: OBJECTIVE
  The aim of this study was to determine the in vitro activity of six new antimicrobial agents against glycopeptide-resistant enterococci (GRE) strains from France.
  
  
  METHODS
  Sixty epidemiologically unrelated clinical isolates of Enterococcus faecium (vanA or vanB), received at the National Reference Centre for Enterococci (CNR-Enc) between 2006 and 2008, were studied. The MICs of the following antibiotics were determined by broth microdilution according to Antibiogram Committee of the French Society for Microbiology (CA-SFM) guidelines: quinupristin-dalfopristin (Q-D), linezolid (LZD), daptomycin (DPT), tigecycline (TGC), ceftobiprole (CFT), and telavancin (TLV). Strains were classified using clinical breakpoints recommended by the CA-SFM (Q-D, LZD, TGC), or the Clinical and Laboratory Standards Institute (DPT).
  
  
  RESULTS
  All strains were susceptible to LZD and DPT (MIC(90), 4 and 2μg/ml, respectively) and only a single strain presented intermediate susceptibility to tigecycline (MIC(90), 0.25μg/ml). Thirty percent of strains were resistant to Q-D (MIC(90), 4μg/ml), and CFT was constantly inactive (MIC(90), 64μg/ml). Finally, TLV showed low-level MICs (MIC(90), 0.5μg/ml) against vanB-positive isolates but not against vanA-positive isolates (MIC(90), 8μg/ml).
  
  
  CONCLUSIONS
  Although several antibiotics are still active against GRE, it is essential to maintain an active antimicrobial resistance surveillance for these microorganisms considered as a model of multidrug resistance with a potential to transfer resistance to other bacterial species (e.g. Staphylococcus aureus).
authors.affiliation: Centre national de référence de la résistance aux antibiotiques, laboratoire associé entérocoques, service de microbiologie, CHU Côte-de-Nacre, avenue de la Côte-de-Nacre, 14033 Caen cedex 09, France.
authors.forename: R
authors.initials: R
authors.lastname: Bérenger
authors.forename: N
authors.initials: N
authors.lastname: Bourdon
authors.forename: M
authors.initials: M
authors.lastname: Auzou
authors.forename: R
authors.initials: R
authors.lastname: Leclercq
authors.forename: V
authors.initials: V
authors.lastname: Cattoir
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D016984:Enterococcus faecium / Q000187:drug effects*; D005602:France; D006020:Glycopeptides / Q000494:pharmacology; D006801:Humans; D013997:Time Factors
chemical_list: D000890:Anti-Infective Agents; D006020:Glycopeptides