title: Identification of T helper type 1-like, Foxp3+ regulatory T cells in human autoimmune disease.
abstract: CD4(+)CD25(high)CD127(low/-) forkhead box p3 (Foxp3)(+) regulatory T cells (T(reg) cells) possess functional plasticity. Here we describe a higher frequency of T helper type 1 (T(H)1)-like, interferon-γ (IFN-γ)-secreting Foxp3(+) T cells in untreated subjects with relapsing remitting multiple sclerosis (RRMS) as compared to healthy control individuals. In subjects treated with IFN-β, the frequency of IFN-γ(+)Foxp3(+) T cells is similar to that in healthy control subjects. In vitro, human T(reg) cells from healthy subjects acquire a T(H)1-like phenotype when cultured in the presence of interleukin-12 (IL-12). T(H)1-like T(reg) cells show reduced suppressive activity in vitro, which can partially be reversed by IFN-γ-specific antibodies or by removal of IL-12.
authors.affiliation: Department of Neurology and Immunobiology, Yale School of Medicine, New Haven, Connecticut, USA.
authors.forename: Margarita
authors.initials: M
authors.lastname: Dominguez-Villar
authors.forename: Clare M
authors.initials: CM
authors.lastname: Baecher-Allan
authors.forename: David A
authors.initials: DA
authors.lastname: Hafler
mesh_terms: D001323:Autoantibodies / Q000276:immunology; D051858:Forkhead Transcription Factors / Q000276:immunology* / Q000502:physiology; D006801:Humans; D007111:Immunity, Cellular / Q000276:immunology; D016899:Interferon-beta / Q000627:therapeutic use; D007371:Interferon-gamma / Q000276:immunology; D018664:Interleukin-12 / Q000502:physiology; D020529:Multiple Sclerosis, Relapsing-Remitting / Q000188:drug therapy / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000502:physiology; D018417:Th1 Cells / Q000187:drug effects / Q000276:immunology* / Q000502:physiology
chemical_list: D001323:Autoantibodies; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D018664:Interleukin-12; D016899:Interferon-beta; D007371:Interferon-gamma