title: Comparative Study of Serological Tests for Mycoplasma synoviae Diagnosis in Commercial Poultry Breeders.
abstract: Avian mycoplasmosis causes great economic losses to the poultry industry, and one of the major agents involved is Mycoplasma synovie (MS). Serum from commercial poultry breeders (n = 2781) was tested for MS by serum plate agglutination (SPA), hemagglutination inhibition (HI), and enzyme-linked immunosorbent assay (ELISA). From 2,781 samples tested, 736 (26.46%) were positive in SPA. From 712 SPA-positive sera, 30 samples (4.21%) were positive in HI, and 150 samples (21.06%) were positive in ELISA. Copositivity between ELISA and HI was 90%, and conegativity was 82.0%. Agreement between HI and ELISA was rejected by McNemar's test (P ≤ .001), and Kappa coefficient showed a weak correlation between the two techniques (k = 0.25; 0.21 ≤ k < 0.40). Weak statistical correlation was observed between all serological tests (SPA, HI, and ELISA), and they should only be used for initial screening for MS.
authors.affiliation: Centro Avançado de Pesquisa Tecnológica do Agronegócio Avícola, Instituto Biológico, Rua Bezerra Paes, 2278, Jardim Belém, 13.690-000 Descalvado, SP, Brazil.
authors.forename: R L
authors.initials: RL
authors.lastname: Luciano
authors.forename: A L S P
authors.initials: AL
authors.lastname: Cardoso
authors.forename: G F Z
authors.initials: GF
authors.lastname: Stoppa
authors.forename: A M I
authors.initials: AM
authors.lastname: Kanashiro
authors.forename: A G M
authors.initials: AG
authors.lastname: de Castro
authors.forename: E N C
authors.initials: EN
authors.lastname: Tessari