title: A miR-125b binding site polymorphism in bone morphogenetic protein membrane receptor type IB gene and prostate cancer risk in China.
abstract: Recently, a C>T polymorphism (rs1434536) in a miR-125b binding site in the 3' untranslated region (3'UTR) of bone morphogenetic protein membrane receptor type IB gene (BMPR1B) has been found to contribute to cancer susceptibility. To investigate whether it plays an important role in the development of prostate cancer in southern Chinese Han population, we performed a case-control study. 247 prostate cancer and 278 control subjects were included in the cancer association study and dual-luciferase reporter assay was used to test the binding ability of miR-125b to BMPR1B-C or -T vectors. The effect of CT/TT genotype on prostate cancer risk was found to be significant for localized disease (OR=1.60, 95% CI=1.01-2.53, P=0.044) and among subgroups of aged>70 years (OR=1.90, 95% CI=1.15-3.15, P=0.015) compared with CC genotype. Moreover, C-allele gave a reduced luciferase activity relative to T-allele in dual-luciferase reporter assay. Our findings show that rs1434536 in the 3'UTR of BMPR1B gene affects the binding ability of miR-125b to BMPR1B mRNA and contributes to the genetic predisposition to localized prostate cancer and patients aged>70 years.
authors.affiliation: Department of Urology, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, China.
authors.forename: Ninghan
authors.initials: N
authors.lastname: Feng
authors.forename: Bin
authors.initials: B
authors.lastname: Xu
authors.forename: Jun
authors.initials: J
authors.lastname: Tao
authors.forename: Pengchao
authors.initials: P
authors.lastname: Li
authors.forename: Gong
authors.initials: G
authors.lastname: Cheng
authors.forename: Zhichao
authors.initials: Z
authors.lastname: Min
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Mi
authors.forename: Meilin
authors.initials: M
authors.lastname: Wang
authors.forename: Na
authors.initials: N
authors.lastname: Tong
authors.forename: Jialin
authors.initials: J
authors.lastname: Tang
authors.forename: Zhengdong
authors.initials: Z
authors.lastname: Zhang
authors.forename: Hongfei
authors.initials: H
authors.lastname: Wu
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Zengjun
authors.initials: Z
authors.lastname: Wang
authors.forename: Lixin
authors.initials: L
authors.lastname: Hua
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000367:Age Factors; D000368:Aged; D001665:Binding Sites / Q000235:genetics; D052005:Bone Morphogenetic Protein Receptors, Type I / Q000235:genetics*; D016022:Case-Control Studies; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D006801:Humans; D008156:Luciferases; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D011471:Prostatic Neoplasms / Q000235:genetics*
chemical_list: D020413:3' Untranslated Regions; C531888:MIRN125 microRNA, human; D035683:MicroRNAs; D008156:Luciferases; C498214:BMPR1B protein, human; D052005:Bone Morphogenetic Protein Receptors, Type I