title: Biocompatible, biodegradable and thermo-sensitive chitosan-g-poly (N-isopropylacrylamide) nanocarrier for curcumin drug delivery.
abstract: A nano formulation of curcumin loaded biodegradable thermoresponsive chitosan-g-poly (N-isopropylacrylamide) co-polymeric nanoparticles (TRC-NPs) (150 nm) were prepared by ionic cross-linking method and characterized. The in vitro drug release was prominent at above LCST. Cytocompatibility of TRC-NPs (100-1000 μg/ml) on an array of cell line is proved by MTT assay. The drug loaded TRC-NPs showed specific toxicity on cancer cells. The cell uptake studies were confirmed by fluorescent microscopy. Flowcytometric analysis of curcumin loaded TRC-NPs showed increased apoptosis on PC3 cells. These results indicated that TRC-NPs could be a potential nanovehicle for curcumin drug delivery.
authors.affiliation: Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham University, Kochi, India.
authors.forename: N Sanoj
authors.initials: NS
authors.lastname: Rejinold
authors.forename: P R
authors.initials: PR
authors.lastname: Sreerekha
authors.forename: K P
authors.initials: KP
authors.lastname: Chennazhi
authors.forename: S V
authors.initials: SV
authors.lastname: Nair
authors.forename: R
authors.initials: R
authors.lastname: Jayakumar
mesh_terms: D000178:Acrylamides / Q000138:chemical synthesis / Q000378:metabolism*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D003474:Curcumin / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology / Q000633:toxicity; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D016503:Drug Delivery Systems; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size
chemical_list: D000178:Acrylamides; D001672:Biocompatible Materials; D004337:Drug Carriers; D048271:Chitosan; C067295:N-isopropylacrylamide; D003474:Curcumin