title: A soluble acetylcholinesterase provides chemical defense against xenobiotics in the pinewood nematode.
abstract: The pinewood nematode genome encodes at least three distinct acetylcholinesterases (AChEs). To understand physiological roles of the three pinewood nematode AChEs (BxACE-1, BxACE-2, and BxACE-3), BxACE-3 in particular, their tissue distribution and inhibition profiles were investigated. Immunohistochemistry revealed that BxACE-1 and BxACE-2 were distributed in neuronal tissues. In contrast, BxACE-3 was detected from some specific tissues and extracted without the aid of detergent, suggesting its soluble nature unlike BxACE-1 and BxACE-2. When present together, BxAChE3 significantly reduced the inhibition of BxACE-1 and BxACE-2 by cholinesterase inhibitors. Knockdown of BxACE-3 by RNA interference significantly increased the toxicity of three nematicidal compounds, supporting the protective role of BxACE-3 against chemicals. In summary, BxACE-3 appears to have a non-neuronal function of chemical defense whereas both BxACE-1 and BxACE-2 have classical neuronal function of synaptic transmission.
authors.affiliation: Research Institute for Agriculture and Life Science, Seoul National University, Seoul, Korea.
authors.forename: Jae Soon
authors.initials: JS
authors.lastname: Kang
authors.forename: Dae-Weon
authors.initials: DW
authors.lastname: Lee
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Koh
authors.forename: Si Hyeock
authors.initials: SH
authors.lastname: Lee
mesh_terms: D000110:Acetylcholinesterase / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D055785:Gene Knockdown Techniques; D007150:Immunohistochemistry; D009348:Nematoda / Q000187:drug effects* / Q000201:enzymology; D034622:RNA Interference; D012995:Solubility; D015262:Xenobiotics / Q000494:pharmacology*
chemical_list: D015262:Xenobiotics; D000110:Acetylcholinesterase