title: The Jasmonate-ZIM-domain proteins interact with the WD-Repeat/bHLH/MYB complexes to regulate Jasmonate-mediated anthocyanin accumulation and trichome initiation in Arabidopsis thaliana.
abstract: Jasmonates (JAs) mediate plant responses to insect attack, wounding, pathogen infection, stress, and UV damage and regulate plant fertility, anthocyanin accumulation, trichome formation, and many other plant developmental processes. Arabidopsis thaliana Jasmonate ZIM-domain (JAZ) proteins, substrates of the CORONATINE INSENSITIVE1 (COI1)-based SCF(COI1) complex, negatively regulate these plant responses. Little is known about the molecular mechanism for JA regulation of anthocyanin accumulation and trichome initiation. In this study, we revealed that JAZ proteins interact with bHLH (Transparent Testa8, Glabra3 [GL3], and Enhancer of Glabra3 [EGL3]) and R2R3 MYB transcription factors (MYB75 and Glabra1), essential components of WD-repeat/bHLH/MYB transcriptional complexes, to repress JA-regulated anthocyanin accumulation and trichome initiation. Genetic and physiological evidence showed that JA regulates WD-repeat/bHLH/MYB complex-mediated anthocyanin accumulation and trichome initiation in a COI1-dependent manner. Overexpression of the MYB transcription factor MYB75 and bHLH factors (GL3 and EGL3) restored anthocyanin accumulation and trichome initiation in the coi1 mutant, respectively. We speculate that the JA-induced degradation of JAZ proteins abolishes the interactions of JAZ proteins with bHLH and MYB factors, allowing the transcriptional function of WD-repeat/bHLH/MYB complexes, which subsequently activate respective downstream signal cascades to modulate anthocyanin accumulation and trichome initiation.
authors.affiliation: School of Life Sciences, Tsinghua University, Beijing, China.
authors.forename: Tiancong
authors.initials: T
authors.lastname: Qi
authors.forename: Susheng
authors.initials: S
authors.lastname: Song
authors.forename: Qingcuo
authors.initials: Q
authors.lastname: Ren
authors.forename: Dewei
authors.initials: D
authors.lastname: Wu
authors.forename: Huang
authors.initials: H
authors.lastname: Huang
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.forename: Meng
authors.initials: M
authors.lastname: Fan
authors.forename: Wen
authors.initials: W
authors.lastname: Peng
authors.forename: Chunmei
authors.initials: C
authors.lastname: Ren
authors.forename: Daoxin
authors.initials: D
authors.lastname: Xie
mesh_terms: D020816:Amino Acid Motifs; D000872:Anthocyanins / Q000378:metabolism*; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism; D003517:Cyclopentanes / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D054883:Oxylipins / Q000378:metabolism*; D010641:Phenotype; D010937:Plant Growth Regulators / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D000872:Anthocyanins; D029681:Arabidopsis Proteins; D051792:Basic Helix-Loop-Helix Proteins; D003517:Cyclopentanes; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D054883:Oxylipins; D010937:Plant Growth Regulators; D012097:Repressor Proteins; D014157:Transcription Factors; C112279:COI1 protein, Arabidopsis; C528950:EGL3 protein, Arabidopsis; C417284:GL3 protein, Arabidopsis; C525049:JAS1 protein, Arabidopsis; C554741:JAZ1 protein, Arabidopsis; C502612:AT1G56650 protein, Arabidopsis; C417767:TT8 protein, Arabidopsis; C071556:GL1 protein, Arabidopsis; C011006:jasmonic acid