title: Evaluation of BacT/Alert 3D automated unit for detection of nontuberculous mycobacteria requiring incubation at 30 degrees C for optimal growth.
abstract: The reliability of the BacT/Alert 3D unit for automated detection of nontuberculous mycobacteria (NTM) that grow optimally at 30 °C was assessed. This system reliably maintained a temperature of 30 °C and detected 50% of the clinical NTM strains (5 Mycobacterium marinum and 3 Mycobacterium gordonae strains) faster than 37 °C culture.
authors.affiliation: Department of Medical Microbiology, University of Manitoba, Winnipeg, Canada. malfa@sbgh.mb.ca
authors.forename: Michelle J
authors.initials: MJ
authors.lastname: Alfa
authors.forename: Kanchana
authors.initials: K
authors.lastname: Manickam
authors.forename: Shadi
authors.initials: S
authors.lastname: Sepehri
authors.forename: Denise
authors.initials: D
authors.lastname: Sitter
authors.forename: Pat
authors.initials: P
authors.lastname: Lenton
mesh_terms: D001331:Automation / Q000379:methods*; D001431:Bacteriological Techniques / Q000379:methods*; D006801:Humans; D009164:Mycobacterium Infections / Q000175:diagnosis*; D019910:Mycobacterium marinum / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification*; D009170:Nontuberculous Mycobacteria / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D013696:Temperature