title: Determination of glyceryl trinitrate and its two main metabolites in human plasma using a new sensitive gas chromatography method.
abstract: The aim of the present study was to determine the concentrations of nitroglycerin (glyceryl trinitrate, GTN, CAS 55-63-0) and its two main stable metabolites; 1,2-dinitroglycerin (1,2-glyceryl dinitrate, GDN, CAS 621-65-8) and 1,3-dinitroglycerin (1,3-GDN, CAS 623-87-0) in human plasma using a capillary gas chromatography method with an electron capture detection. Using the GC conditions, linear calibrations were obtained for 1,3-GDN from 0.14 to 3 ng/mL, for 1,2-GDN from 0.06 to 6 ng/mL, and for GTN from 0.01 to 0.3 ng/mL in plasma samples by the following calibration curve equations: [y = 0.1924x - 0.0088 (r = 0.999)], [y = 0.2273x + 0.0164 (r = 0.995)], [y = 17.434x - 0.0751] for 1,3-GDN, 1,2-GDN, and GTN respectively. The calculated limits of quantification values for GTN, 1,2-GDN, and 1,3-GDN were 0.03 ng/mL, 0.2 ng/mL, and 0.15 ng/mL respectively. This method was verified with a bioequivalence study of an Iranian brand of oral sustained release nitroglycerin with an innovator formulation.
authors.affiliation: Faculty of Pharmacy, Tabriz University of Medical Science, Tabriz, Iran.
authors.forename: Mohammadreza
authors.initials: M
authors.lastname: Ghandforoush-Sattari
authors.forename: Parvin
authors.initials: P
authors.lastname: Zakeri-Milani
authors.forename: Siminozar
authors.initials: S
authors.lastname: Mashayekhi
authors.forename: Hadi
authors.initials: H
authors.lastname: Valizadeh
mesh_terms: D000279:Administration, Cutaneous; D000328:Adult; D019540:Area Under Curve; D001682:Biological Availability; D001711:Biotransformation; D002138:Calibration; D002849:Chromatography, Gas; D006801:Humans; D008297:Male; D005996:Nitroglycerin / Q000031:analogs & derivatives / Q000097:blood* / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D012997:Solvents; D013810:Therapeutic Equivalency; D014665:Vasodilator Agents / Q000097:blood* / Q000493:pharmacokinetics; D055815:Young Adult
chemical_list: D012997:Solvents; D014665:Vasodilator Agents; C014509:dinitroglycerol; D005996:Nitroglycerin