title: Molecular and Biological Characterization of Chinese Sacbrood Virus LN Isolate.
abstract: Chinese sacbrood virus (CSBV) was purified from diseased insects, and its genome was cloned and sequenced. The genomic RNA of CSBV is 8863 nucleotides in length and contains a single large open reading frame encoding a 319.614 kDa polyprotein. The coding sequence is flanked by a 178-nucleotide 5' nontranslated leader sequence and a 142-nucleotide 3' nontranslated region, followed a poly(A) tail. Four major structural proteins, VP1,VP2, VP3 and VP4, were predicted in the N-teminal of the polyprotein. The C-terminal part of the polyprotein contains sequence motifs which is a typical and well-characterized picornavirus nonstructural proteins: an RNA helicase, a chymotrypsin-like 3C protease, and an RNA-dependent RNA polymerase. Genetic analysis shows that the CSBV-LN had a 13-amino-acid deletion at amino acid positions 710-719 and 727-729 in comparison with CSBV-GZ and SBV-UK, and the SBV-UK had a 7-amino-acid deletion at amino acid positions 2124-2132 in comparison with CSBV-GZ and CSBV-LN, and the CSBV-GZ and CSBV-LN had a 6-amino-acid deletion at amino acid positions 2143-2150 in comparison with SBV-UK. Phylogenetic analysis using RdRp of selected picorna-like viruses shows that CSBV/SBV and Deformed Wing Virus (DWV) tend to group together, which possesses an RNA of similar size and gene order.
authors.affiliation: Laboratory Animal Center, Liaoning Medical University, Jinzhou 121001, China.
authors.forename: Ma
authors.initials: M
authors.lastname: Mingxiao
authors.forename: Li
authors.initials: L
authors.lastname: Ming
authors.forename: Cheng
authors.initials: C
authors.lastname: Jian
authors.forename: Yang
authors.initials: Y
authors.lastname: Song
authors.forename: Wang
authors.initials: W
authors.lastname: Shude
authors.forename: Li
authors.initials: L
authors.lastname: Pengfei