title: Identification of PhoB binding sites of the yibD and ytfK promoter regions in Escherichia coli.
abstract: By using a lacZ operon fusion genomic library of the Escherichia coli 0157:H7 Sakai, we identified phosphate-starvation-inducible (psi) promoters located upstream of the yibD and ytfK genes. They have been previously proposed to belong to the phosphate regulon (pho regulon) by Beak and Lee (2006), based on the DNA array and in vivo transcriptional experiments. However, the direct interaction of these promoters with the activator protein of the pho regulon, PhoB, has not been determined. We determined the binding regions of PhoB in these promoter regions by DNase I footprinting. Both regions contained two pho boxes similar to the consensus sequence for PhoB binding.
authors.affiliation: Department of Applied Chemistry, National Defense Academy, Yokosuka 239-8686, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Yoshida
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Sugiyama
authors.forename: Tomoya
authors.initials: T
authors.lastname: Oyamada
authors.forename: Katsushi
authors.initials: K
authors.lastname: Yokoyama
authors.forename: Soo-Ki
authors.initials: SK
authors.lastname: Kim
authors.forename: Kozo
authors.initials: K
authors.lastname: Makino
mesh_terms: D019677:Artificial Gene Fusion; D001426:Bacterial Proteins / Q000378:metabolism*; D001665:Binding Sites; D018983:DNA Footprinting; D003850:Deoxyribonuclease I / Q000378:metabolism; D019453:Escherichia coli O157 / Q000235:genetics* / Q000378:metabolism; D005798:Genes, Bacterial / Q000235:genetics*; D017930:Genes, Reporter; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; C061278:PhoB protein, Bacteria; D003850:Deoxyribonuclease I; D001616:beta-Galactosidase