title: msl2 mRNA is bound by free nuclear MSL complex in Drosophila melanogaster.
abstract: In Drosophila, the global increase in transcription from the male X chromosome to compensate for its monosomy is mediated by the male-specific lethal (MSL) complex consisting of five proteins and two non-coding RNAs, roX1 and roX2. After an initial sequence-dependent recognition by the MSL complex of 150-300 high affinity sites, the spread to the majority of the X-linked genes depends on local MSL-complex concentration and active transcription. We have explored whether any additional RNA species are associated with the MSL complex. No additional roX RNA species were found, but a strong association was found between a spliced and poly-adenylated msl2 RNA and the MSL complex. Based on our results, we propose a model in which a non-chromatin-associated partial or complete MSL-complex titrates newly transcribed msl2 mRNA and thus regulates the amount of available MSL complex by feedback. This represents a novel mechanism in chromatin structure regulation.
authors.affiliation: Department of Molecular Biology, Umeå University, SE-90187 Umeå, Sweden.
authors.forename: Anna-Mia
authors.initials: AM
authors.lastname: Johansson
authors.forename: Anders
authors.initials: A
authors.lastname: Allgardsson
authors.forename: Per
authors.initials: P
authors.lastname: Stenberg
authors.forename: Jan
authors.initials: J
authors.lastname: Larsson
mesh_terms: D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism; D004331:Drosophila melanogaster / Q000235:genetics* / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014960:X Chromosome
chemical_list: D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D009687:Nuclear Proteins; C092595:Pabp2 protein, Drosophila; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D014157:Transcription Factors; C094166:msl-2 protein, Drosophila; C454144:roX1 protein, Drosophila