title: Multiple tumor-suppressor-1 gene and esophageal-carcinoma.
abstract: The multiple tumor suppressor 1 (MTS1) gene is homozygously deleted frequently in cell lines derived from a wide variety of tumors. We investigated the deletion of the MTS1 gene in esophageal cancer cell lines and primary esophageal squamous carcinomas using the polymerase chain reaction. Sixteen and 15 of 23 esophageal cancer cell lines showed homozygous deletion of MTS1 exon 1 and exon 2, respectively, while none of 21 primary esophageal carcinomas showed the deletion. An analysis of MTS1 gene mutations was carried out by direct DNA sequencing in 8 cell lines and 21 primary carcinomas showing no homozygous deletion. In contrast to previous reports of esophageal carcinoma, there were no mutations recognized in the region sequenced. Our study suggests that the inactivation of the MTS 1 gene may play an important role in esophageal carcinoma cell lines but may be less important in primary carcinomas of the human esophagus.
authors.affiliation: KYUSHU UNIV,MED INST BIOREGULAT,DEPT SURG,BEPPU,OITA 874,JAPAN. SAITAMA CANC CTR,DEPT SURG,INA,SAITAMA,JAPAN. KYOTO UNIV,FAC MED,DEPT SURG 1,KYOTO,JAPAN. UNIV MASSACHUSETTS,MED CTR,DIV DIGEST DIS & NUTR,WORCESTER,MA. KYUSHU UNIV,SCH MED,DEPT SURG 2,FUKUOKA 812,JAPAN.
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.forename: M
authors.initials: M
authors.lastname: Mori
authors.forename: Y
authors.initials: Y
authors.lastname: Yang
authors.forename: H
authors.initials: H
authors.lastname: Inoue
authors.forename: K
authors.initials: K
authors.lastname: Mimori
authors.forename: K
authors.initials: K
authors.lastname: Shibuta
authors.forename: H
authors.initials: H
authors.lastname: Nakashima
authors.forename: K
authors.initials: K
authors.lastname: Mafune
authors.forename: Y
authors.initials: Y
authors.lastname: Shimada
authors.forename: G
authors.initials: G
authors.lastname: Barnard
authors.forename: K
authors.initials: K
authors.lastname: Sugimachi
authors.forename: T
authors.initials: T
authors.lastname: Akiyoshi