title: Targeting of HIV-p24 particle-based vaccine into differential skin layers induces distinct arms of the immune responses.
abstract: Skin routes of immunization such as subcutaneous (SC), intradermal (ID) and transcutaneous (TC) administration are utilized for vaccination against various pathogens, without understanding their potential impact on the outcome of immune responses. We demonstrated that SC immunization induced HIV-1 p24 specific IgG in absence of antigen-specific CD8 T cells, whereas the ID route induced both cellular and humoral responses. Interestingly, TC application through empty hair follicular ducts, targeting epidermal Langerhans Cells (LCs), induced major CD8 effector cells, in the absence of IgG. However, high levels of mucosal IgA, were localized in the stratified epithelium of the vagina after TC prime. We propose that re-directing the immune responses by targeting differential skin immunization routes, offers enormous potential for innovative vaccination strategies, especially against HIV.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale, INSERM UMR-S 945 and Université Pierre et Marie Curie (UPMC University Paris 06), Laboratory of Immunity and Infection, 83/91, Boulevard de l'Hôpital, 75013 Paris Cedex 13, France.
authors.forename: Christelle
authors.initials: C
authors.lastname: Liard
authors.forename: Séverine
authors.initials: S
authors.lastname: Munier
authors.forename: Mauricio
authors.initials: M
authors.lastname: Arias
authors.forename: Alix
authors.initials: A
authors.lastname: Joulin-Giet
authors.forename: Olivia
authors.initials: O
authors.lastname: Bonduelle
authors.forename: Darragh
authors.initials: D
authors.lastname: Duffy
authors.forename: Robin J
authors.initials: RJ
authors.lastname: Shattock
authors.forename: Bernard
authors.initials: B
authors.lastname: Verrier
authors.forename: Behazine
authors.initials: B
authors.lastname: Combadière
mesh_terms: D016915:AIDS Vaccines / Q000008:administration & dosage* / Q000276:immunology*; D000279:Administration, Cutaneous; D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D015483:HIV Antibodies / Q000276:immunology; D016655:HIV Core Protein p24 / Q000276:immunology*; D015497:HIV-1; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D053758:Nanoparticles; D012867:Skin / Q000276:immunology / Q000821:virology; D014611:Vaccination
chemical_list: D016915:AIDS Vaccines; D016207:Cytokines; D015483:HIV Antibodies; D016655:HIV Core Protein p24; D007070:Immunoglobulin A; D007074:Immunoglobulin G