title: Optimal phenotypic testing of AmpC beta-lactamases using boronic acid solutions.
abstract: Plasmid-mediated class C β-lactamases are reported from Escherichia coli and Klebsiella pneumoniae with increasing frequency. No screening and confirmatory tests have been uniformly established for these strains. We investigated for the presence of plasmid-mediated AmpC production in 51 clinical isolates of Enterobacteriaceae, comparing two different boronic acid formulations, phenylboronic acid (PB) and 3-(N-Boc-amino)phenylboronic acid (APB), using polymerase chain reaction (PCR). PB performed better than APB.
authors.affiliation: Department of Microbiology, Concord Hospital, Sydney, NSW, Australia. Yuen.Su@sswahs.nsw.gov.au
authors.forename: W-Y
authors.initials: WY
authors.lastname: Su
authors.forename: T
authors.initials: T
authors.lastname: Gottlieb
authors.forename: J
authors.initials: J
authors.lastname: Merlino
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001897:Boronic Acids; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D010641:Phenotype; D010957:Plasmids; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D001897:Boronic Acids; C048795:AmpC beta-lactamases; D001618:beta-Lactamases