title: Absorption of 6-O-caffeoylsophorose and its metabolites in Sprague-Dawley rats detected by electrochemical detector-high-performance liquid chromatography and electrospray ionization-time-of-flight-mass spectrometry methods.
abstract: Absorption and metabolism of a natural compound, 6-O-caffeoylsophorose (CS) from acylated anthocyanins in a red vinegar fermented with purple sweet potato, were clarified. The absorption of CS and conjugated CS in blood from orally administrated Sprague-Dawley rats at a dose of 400 mg/kg was investigated by electrochemical detection-high performance liquid chromatography. As a result, CS was successfully detected in rat plasma (AUC(0-6h), 108.6 ± 8.1 nmol h/mL) and was found to be an intact absorbable polyphenol. In addition, half of the absorbed CS was detected as its conjugates (AUC(0-6h), 50.7 ± 5.7 nmol h/mL) as well as caffeic and ferulic acids from CS. By a time-of-flight-mass spectrometric analysis of CS-administered plasma sample, glucuronide and methylated conjugates of CS were identified, in addition to glucuronide, methylated, or sulfate conjugates of caffeic and ferulic acids. Consequently, CS was absorbed in intact form into rat blood and partly degraded to caffeic and ferulic acids or metabolized by glucuronidation, methylation, or sulfatation.
authors.affiliation: Department of Bioscience and Biotechnology, Division of Bioresource and Bioenvironmental Sciences, Faculty of Agriculture, Graduated School of Kyushu University, 6-10-1 Hakozaki, Fukuoka 812-8581, Japan.
authors.forename: Ju
authors.initials: J
authors.lastname: Qiu
authors.forename: Nozomi
authors.initials: N
authors.lastname: Saito
authors.forename: Mai
authors.initials: M
authors.lastname: Noguchi
authors.forename: Keiichi
authors.initials: K
authors.lastname: Fukui
authors.forename: Kayo
authors.initials: K
authors.lastname: Yoshiyama
authors.forename: Kazusato
authors.initials: K
authors.lastname: Matsugano
authors.forename: Norihiko
authors.initials: N
authors.lastname: Terehara
authors.forename: Toshiro
authors.initials: T
authors.lastname: Matsui
mesh_terms: D000042:Absorption; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004187:Disaccharides / Q000097:blood* / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: C475258:6-O-caffeoyl-(2-O-glucopyranosyl)-glucopyranose; D004187:Disaccharides