title: Ultra-performance ion-pairing liquid chromatography with on-line electrospray ion trap mass spectrometry for heparin disaccharide analysis.
abstract: A high-resolution method for the separation and analysis of disaccharides prepared from heparin and heparan sulfate (HS) using heparin lyases is described. Ultra-performance liquid chromatography in a reverse-phase ion-pairing mode efficiently separates eight heparin/HS disaccharides. The disaccharides can then be detected and quantified using electrospray ionization mass spectrometry. This method is particularly useful in the analysis of small amounts of biological samples, including cells, tissues, and biological fluids, because it provides high sensitivity without being subject to interference from proteins, peptides, and other sample impurities.
authors.affiliation: Department of Chemistry and Chemical Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA.
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.forename: Amanda
authors.initials: A
authors.lastname: Weyers
authors.forename: Jong Youn
authors.initials: JY
authors.lastname: Baik
authors.forename: Eric
authors.initials: E
authors.lastname: Sterner
authors.forename: Susan
authors.initials: S
authors.lastname: Sharfstein
authors.forename: Shaker A
authors.initials: SA
authors.lastname: Mousa
authors.forename: Fuming
authors.initials: F
authors.lastname: Zhang
authors.forename: Jonathan S
authors.initials: JS
authors.lastname: Dordick
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Linhardt
mesh_terms: D000818:Animals; D016466:CHO Cells; D002162:Camelus; D002853:Chromatography, Liquid / Q000379:methods*; D006224:Cricetinae; D003412:Cricetulus; D004187:Disaccharides / Q000032:analysis* / Q000302:isolation & purification; D006493:Heparin / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification; D019763:Heparin Lyase / Q000378:metabolism; D006495:Heparin, Low-Molecular-Weight / Q000032:analysis; D006497:Heparan Sulfate / Q000032:analysis; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D004187:Disaccharides; D006493:Heparin; D019763:Heparin Lyase; D006495:Heparin, Low-Molecular-Weight; D006497:Heparan Sulfate; C464536:heparin disaccharide