title: Extraction of proteins with low fluoride level from Antarctic krill (Euphausia superba) and their composition analysis.
abstract: The extraction of proteins with low fluoride level (LFP) from Antarctic krill (Euphausia superba) was investigated in this work. The optimal conditions for protein solubilization were determined to be pH 11.5 and 4 °C. The proteins were solubilized two times; a water/krill ratio (mL/g) of 6 and a time of 30 min were used for the first step, whereas the second used a water/krill residue ratio (mL/g) of 3 and a time of 30 min. The optimum pH for protein precipitation was 4.6. A LFP with fluoride content of 9.86 mg/kg (dry weight) was finally obtained through a fluoride removal program. The protein yield of LFP was 52.68%. Composition analysis of LFP indicated it was composed of 66.96% of crude proteins (dry weight) and 33.01% of total lipids (dry weight),, and all nine essential amino acids were in sufficient amounts to meet FAO/WHO/UNU requirements for adults and infants. In addition, LFP could be taken as a good source of EPA and DHA for consideration of use as a food item for human consumption.
authors.affiliation: College of Food Science and Engineering, Ocean University of China, Qingdao 266003, People's Republic of China.
authors.forename: Lingzhao
authors.initials: L
authors.lastname: Wang
authors.forename: Changhu
authors.initials: C
authors.lastname: Xue
authors.forename: Yuming
authors.initials: Y
authors.lastname: Wang
authors.forename: Bao
authors.initials: B
authors.lastname: Yang
mesh_terms: D000818:Animals; D000864:Antarctic Regions; D005591:Chemical Fractionation; D033201:Euphausiacea / Q000737:chemistry*; D005459:Fluorides / Q000032:analysis*; D011506:Proteins / Q000032:analysis* / Q000302:isolation & purification*
chemical_list: D011506:Proteins; D005459:Fluorides