title: Expression and distribution of PPP2R5C gene in leukemia.
abstract: BACKGROUND
  Recently, we clarified at the molecular level novel chromosomal translocation t(14;14)(q11;q32) in a case of Sézary syndrome, which caused a rearrangement from TRAJ7 to the PPP2R5C gene. PPP2R5C is one of the regulatory B subunits of protein phosphatase 2A (PP2A). It plays a crucial role in cell proliferation, differentiation, and transformation. To characterize the expression and distribution of five different transcript variants of the PPP2R5C gene in leukemia, we analyzed the expression level of PPP2R5C in peripheral blood mononuclear cells from 77 patients with de novo leukemia, 26 patients with leukemia in complete remission (CR), and 20 healthy individuals by real-time PCR and identified the different variants of PPP2R5C by RT-PCR.
  
  
  RESULTS
  Significantly higher expression of PPP2R5C was found in AML, CML, T-ALL, and B-CLL groups in comparison with healthy controls. High expression of PPP2R5C was detected in the B-ALL group; however, no significant difference was found compared with the healthy group. The expression level of PPP2R5C in the CML-CR group decreased significantly compared with that in the de novo CML group and was not significantly different from the level in the healthy group. By using different primer pairs that covered different exons, five transcript variants of PPP2R5C could be identified. All variants could be detected in healthy samples as well as in all the leukemia samples, and similar frequencies and distributions of PPP2R5C were indicated.
  
  
  CONCLUSIONS
  Overexpression of PPP2R5C in T-cell malignancy as well as in myeloid leukemia cells might relate to its proliferation and differentiation. Investigation of the effect of target inhibition of this gene might be beneficial to further characterization of molecular mechanisms and targeted therapy in leukemia.
authors.affiliation: Institute of Hematology, Medical College, Jinan University, Guangzhou, People's Republic of China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Zheng
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
authors.forename: Shaohua
authors.initials: S
authors.lastname: Chen
authors.forename: Yuzhe
authors.initials: Y
authors.lastname: Niu
authors.forename: Lijian
authors.initials: L
authors.lastname: Yang
authors.forename: Bo
authors.initials: B
authors.lastname: Li
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Lu
authors.forename: Suxia
authors.initials: S
authors.lastname: Geng
authors.forename: Xin
authors.initials: X
authors.lastname: Du
authors.forename: Yangqiu
authors.initials: Y
authors.lastname: Li
mesh_terms: D014408:Biomarkers, Tumor / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology; D045744:Cell Line, Tumor; D015870:Gene Expression; D006801:Humans; D019169:Jurkat Cells; D020014:K562 Cells; D007938:Leukemia / Q000201:enzymology* / Q000235:genetics* / Q000473:pathology; D054648:Protein Phosphatase 2 / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; C503981:PPP2R5C protein, human; D054648:Protein Phosphatase 2