title: ASPS-1, a novel cell line manifesting key features of alveolar soft part sarcoma.
abstract: In vitro growth of alveolar soft part sarcoma (ASPS) and establishment of an ASPS cell line, ASPS-1, are described in this study. Using a recently developed xenograft model of ASPS derived from a lymph node metastasis, organoid nests consisting of 15 to 25 ASPS cells were isolated from ASPS xenograft tumors by capture on 70 μm filters and plated in vitro. After attachment to the substratum, these nests deposited small aggregates of ASPS cells. These cells grew slowly and were expanded over a period of 3 years and have maintained characteristics consistent with those of both the original ASPS tumor from the patient and the xenograft tumor including (1) presence of the alveolar soft part locus-transcription factor E3 type 1 fusion transcript and nuclear expression of the alveolar soft part locus-transcription factor E3 type 1 fusion protein; (2) maintenance of the t(X;17)(p11;q25) translocation characteristic of ASPS; and (3) expression of upregulated ASPS transcripts involved in angiogenesis (ANGPTL2, HIF-1-α, MDK, c-MET, VEGF, and TIMP-2), cell proliferation (PRL, PCSK1), metastasis (ADAM9), as well as the transcription factor BHLHB3 and the muscle-specific transcripts TRIM63 and ITGβ1BP3. This ASPS cell line forms colonies in soft agar and retains the ability to produce highly vascularized ASPS tumors in NOD.SCID/NCr mice. Immunohistochemistry of selected ASPS markers on these tumors indicated similarity to those of the original patient tumor as well as to the xenografted ASPS tumor. We anticipate that this ASPS cell line will accelerate investigations into the biology of ASPS including identification of new therapeutic approaches for treatment of this slow growing soft tissue sarcoma.
authors.affiliation: Screening Technologies Branch, Developmental Therapeutics Program, National Cancer Institute at Frederick, Frederick, MD 21702, USA.
authors.forename: Susan
authors.initials: S
authors.lastname: Kenney
authors.forename: David T
authors.initials: DT
authors.lastname: Vistica
authors.forename: Luke H
authors.initials: LH
authors.lastname: Stockwin
authors.forename: Sandra
authors.initials: S
authors.lastname: Burkett
authors.forename: Melinda G
authors.initials: MG
authors.lastname: Hollingshead
authors.forename: Suzanne D
authors.initials: SD
authors.lastname: Borgel
authors.forename: Donna O
authors.initials: DO
authors.lastname: Butcher
authors.forename: David S
authors.initials: DS
authors.lastname: Schrump
authors.forename: Robert H
authors.initials: RH
authors.lastname: Shoemaker
mesh_terms: D000328:Adult; D000818:Animals; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors / Q000235:genetics; D015197:Carcinogenicity Tests; D002455:Cell Division / Q000502:physiology; D045744:Cell Line, Tumor; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D066298:In Vitro Techniques; D047908:Intracellular Signaling Peptides and Proteins; D008198:Lymph Nodes / Q000473:pathology*; D008207:Lymphatic Metastasis; D008297:Male; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D015514:Oncogene Proteins, Fusion / Q000235:genetics; D018234:Sarcoma, Alveolar Soft Part / Q000235:genetics* / Q000556:secondary*; D012983:Soft Tissue Neoplasms / Q000235:genetics* / Q000473:pathology*; D014178:Translocation, Genetic; D014183:Transplantation, Heterologous
chemical_list: C422743:ASPSCR1 protein, human; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; D047908:Intracellular Signaling Peptides and Proteins; D015514:Oncogene Proteins, Fusion; C069203:TFE3 protein, human