title: Sialic acid C-glycosides with aromatic residues: investigating enzyme binding and inhibition of Trypanosoma cruzi trans-sialidase.
abstract: Several α-configured C-sialosides were synthesised by cross metathesis and further synthetic derivatisation to obtain ligands for Trypanosoma cruzi trans-sialidase (TcTS), a key enzyme in Chagas disease. Affinities of these compounds to immobilised TcTS were measured by surface plasmon resonance (SPR). The K(D) values thus obtained are in the lower millimolar range and will be discussed. The results show the importance of addressing Tyr(119) and Trp(312) side chains of TcTS in target oriented ligand synthesis, since these amino acids constitute the acceptor binding region in the active site of TcTS. The best ligand showed a significant decrease of TcTS activity in a preliminary NMR based inhibition assay.
authors.affiliation: University of Hamburg, Faculty of Science, Department of Chemistry, Institute of Organic Chemistry, Martin-Luther-King Platz 6, 20146, Hamburg, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Meinke
authors.forename: Andreas
authors.initials: A
authors.lastname: Schroven
authors.forename: Joachim
authors.initials: J
authors.lastname: Thiem
mesh_terms: D001665:Binding Sites; D020134:Catalytic Domain; D014355:Chagas Disease / Q000188:drug therapy* / Q000469:parasitology; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D004791:Enzyme Inhibitors / Q000138:chemical synthesis / Q000494:pharmacology*; D004800:Enzymes, Immobilized / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism; D006023:Glycoproteins / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism; D006027:Glycosides / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007700:Kinetics; D008024:Ligands; D008958:Models, Molecular; D009439:Neuraminidase / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism; D019906:Nuclear Magnetic Resonance, Biomolecular; D011485:Protein Binding; D012794:Sialic Acids / Q000737:chemistry* / Q000378:metabolism; D020349:Surface Plasmon Resonance; D014349:Trypanosoma cruzi / Q000187:drug effects* / Q000201:enzymology
chemical_list: D004791:Enzyme Inhibitors; D004800:Enzymes, Immobilized; D006023:Glycoproteins; D006027:Glycosides; D008024:Ligands; D012794:Sialic Acids; C069715:trans-sialidase; D009439:Neuraminidase