title: Dietary grape-seed procyanidins decreased postweaning diarrhea by modulating intestinal permeability and suppressing oxidative stress in rats.
abstract: This study was conducted to evaluate the effects of grape-seed procyanidins in controlling weaning diarrhea using a rat model. Weaned rats were fed either the basal diet or basal diet supplemented with either 250 mg/kg grape-seed procyanidins or 2000 mg/kg ZnO. Treated rats had better performance with a reduced incidence of diarrhea (P < 0.05). Both ZnO and grape-seed procyanidins significantly reduced urinary lactulose to mannitol ratios (P < 0.05) and enhanced the mRNA and protein expression of the intestinal mucosal tight junction proteins Ocln/ZO-1 (P < 0.05). Grape-seed procyanidins increased the activities of SOD, GSH-Px, and GSH while decreasing the level of MDA in the intestinal mucosa (P < 0.05). Furthermore, an in vitro investigation revealed that supplementation with grape-seed procyanidins in IEC-6 intestinal epithelial cells significantly enhanced the expression of Ocln/ZO-1 under H(2)O(2)-induced oxidative stress. Collectively, these results indicate that grape-seed procyanidins have the potential to prevent weaning diarrhea by reducing intestinal permeability and improving antioxidant indices.
authors.affiliation: State Key Lab of Animal Nutrition, Ministry of Agriculture Feed Industry Centre, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing 100193, People's Republic of China.
authors.forename: Peixia
authors.initials: P
authors.lastname: Song
authors.forename: Ruoji
authors.initials: R
authors.lastname: Zhang
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: Wang
authors.forename: Pingli
authors.initials: P
authors.lastname: He
authors.forename: Lulin
authors.initials: L
authors.lastname: Tan
authors.forename: Xi
authors.initials: X
authors.lastname: Ma
mesh_terms: D000818:Animals; D002460:Cell Line; D002463:Cell Membrane Permeability / Q000187:drug effects; D003967:Diarrhea / Q000188:drug therapy* / Q000378:metabolism*; D019587:Dietary Supplements / Q000032:analysis*; D015536:Down-Regulation / Q000187:drug effects*; D006801:Humans; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D044945:Proanthocyanidins / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012639:Seeds / Q000737:chemistry; D027843:Vitis / Q000737:chemistry*
chemical_list: D044945:Proanthocyanidins