title: [The expression and relationship of CXCR4 and microvessel density in laryngeal squamous cell carcinoma].
abstract: OBJECTIVE
  To investigate the expression of chemokine receptor 4 (CXCR4) in laryngeal squamous carcinoma and its correlation with clinicopathology character and the building of microvessel density (MVD). To evaluate its role in the carcinogenesis and progression in laryngeal squamous carcinoma.
  
  
  METHODS
  The expression of CXCR4 in fresh laryngeal squamous carcinoma tissues and adjacent normal tissues from 42 patients were examined by RT-PCR, immunohistochemical staining and Image-pro-plus software. The numbers of regeneration blood vessels in the laryngeal squamous carcinoma was counted by antibody against factor V associated antigen and immunohistochemical analysis.
  
  
  RESULTS
  The positive expression rate of CXCR4 in tumor samples was significantly higher than that in normal ones (P < 0.01). In tumor samples, the expression of CXCR4 were not associated with age, sex, tumor site and T stage (P > 0.05), while it were higher in tumors of grade III, IV than in grade I, II of pathology classification (P < 0. 01). The expression of CXCR4 were significantly higher in tumors with cervical lymph node metastasis than that in tumor without cervical lymph node metastasis (P < 0.01). The expression of CXCR4 protein and CXCR4 mRNA were at the same level. The expression level of CXCR4 in the laryngeal squamous carcinoma tissue was positively correlated with vascularization.
  
  
  CONCLUSIONS
  The higher expression of CXCR4 may play a key role in the invasion and metastasis of laryngeal squamous carcinoma, and were correlated with micro-vascularization.
authors.affiliation: Department of Head and Neck Surgery, Zhejiang Cancer Hospital, Hangzhou, 310022, China.
authors.forename: Chuanming
authors.initials: C
authors.lastname: Zheng
authors.forename: Rongming
authors.initials: R
authors.lastname: Ge
authors.forename: Minghua
authors.initials: M
authors.lastname: Ge
authors.forename: Xicai
authors.initials: X
authors.lastname: Sun
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000098:blood supply* / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D007822:Laryngeal Neoplasms / Q000098:blood supply* / Q000378:metabolism / Q000473:pathology; D008207:Lymphatic Metastasis; D055806:Microvessels / Q000473:pathology; D008875:Middle Aged; D009367:Neoplasm Staging; D009389:Neovascularization, Pathologic / Q000473:pathology; D019718:Receptors, CXCR4 / Q000378:metabolism*
chemical_list: C527276:CXCR4 protein, human; D019718:Receptors, CXCR4