title: Bovine serum albumin nanoparticle promotes the stability of quercetin in simulated intestinal fluid.
abstract: Quercetin (Que) is a flavonoid widely distributed in vegetables and fruits and exhibits strong antioxidant activity, but the poor stability of Que limits its function and application. The present study developed a nanoparticle (NP) using bovine serum albumin (BSA) as a matrix to encapsulate Que. The stability of encapsulated Que by BSA NP was tracked in a simulated intestinal fluid (SIF). The antioxidant activity of encapsulated Que was evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging assays. Furthermore, the stabilizing mechanism of Que by BSA NP was investigated, using scanning transmisson electron microscopy (STEM), dynamic light scattering (DLS), UV-vis, fluorescence spectrometry, and circular dichroism (CD). The results revealed that Que was effectively encapsulated by BSA and formed spherical NP (<10 nm). BSA NP not only promoted the stability of encapsulated Que but also kept the antioxidant activity of encapsulated Que. The driving forces for BSA-Que association were hydrophobic interaction and hydrogen bond, and the latter was involved in the mechanism of Que stabilization. This suggested that BSA NP could be a good carrier to deliver hydrophobic flavonols.
authors.affiliation: College of Food Science and Nutritional Engineering, China Agricultural University, Key Laboratory of Functional Dairy Science of Beijing and Ministry of Education, Beijing Higher Institution Engineering Research Center of Animal Product, No. 17 Qinghua East Road, Haidian, Beijing 100083, China.
authors.forename: Ru
authors.initials: R
authors.lastname: Fang
authors.forename: Ruifang
authors.initials: R
authors.lastname: Hao
authors.forename: Xia
authors.initials: X
authors.lastname: Wu
authors.forename: Qi
authors.initials: Q
authors.lastname: Li
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Leng
authors.forename: Hao
authors.initials: H
authors.lastname: Jing
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry*; D002417:Cattle; D004339:Drug Compounding / Q000379:methods*; D004355:Drug Stability; D007413:Intestinal Mucosa / Q000378:metabolism*; D008954:Models, Biological; D053758:Nanoparticles / Q000737:chemistry; D011794:Quercetin / Q000737:chemistry* / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D012710:Serum Albumin, Bovine; D011794:Quercetin