title: Interaction of enterocyte FABPs with phospholipid membranes: clues for specific physiological roles.
abstract: Intestinal and liver fatty acid binding proteins (IFABP and LFABP, respectively) are cytosolic soluble proteins with the capacity to bind and transport hydrophobic ligands between different sub-cellular compartments. Their functions are still not clear but they are supposed to be involved in lipid trafficking and metabolism, cell growth, and regulation of several other processes, like cell differentiation. Here we investigated the interaction of these proteins with different models of phospholipid membrane vesicles in order to achieve further insight into their specificity within the enterocyte. A combination of biophysical and biochemical techniques allowed us to determine affinities of these proteins to membranes, the way phospholipid composition and vesicle size and curvature modulate such interaction, as well as the effect of protein binding on the integrity of the membrane structure. We demonstrate here that, besides their apparently opposite ligand transfer mechanisms, both LFABP and IFABP are able to interact with phospholipid membranes, but the factors that modulate such interactions are different for each protein, further implying different roles for IFABP and LFABP in the intracellular context. These results contribute to the proposed central role of intestinal FABPs in the lipid traffic within enterocytes as well as in the regulation of more complex cellular processes.
authors.affiliation: Instituto de Investigaciones Bioquímicas de La Plata, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, La Plata, Argentina.
authors.forename: Lisandro J
authors.initials: LJ
authors.lastname: Falomir-Lockhart
authors.forename: Gisela R
authors.initials: GR
authors.lastname: Franchini
authors.forename: María Ximena
authors.initials: MX
authors.lastname: Guerbi
authors.forename: Judith
authors.initials: J
authors.lastname: Storch
authors.forename: Betina
authors.initials: B
authors.lastname: Córsico
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D055592:Biophysical Phenomena; D045304:Cytochromes c / Q000378:metabolism; D020895:Enterocytes / Q000378:metabolism*; D050556:Fatty Acid-Binding Proteins / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008563:Membrane Lipids / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D053835:Unilamellar Liposomes / Q000378:metabolism
chemical_list: D050556:Fatty Acid-Binding Proteins; D008563:Membrane Lipids; D010743:Phospholipids; D053835:Unilamellar Liposomes; D045304:Cytochromes c