title: Inhibition of migration of MDA-MB-231 cells by methyl-3,5-diiodo-4-(4'-methoxyphenoxy)benzoate (DIME).
abstract: The GTPase activity of purified dimeric tubulin (alpha+beta) at 5 mu M was insensitive to methyl-3,5-diiodo-4-(4'-methoxyphenoxy) benzoate (DIME), in contrast to nocodazole which activated GTPase. Cellular motility of MDA-MB-231 (human mammary cancer) cells migrating through 12-mu m pores was inhibited by DIME similar to nocodazole in a drug concentration-and DIME structure-dependent manner. An increase of cytoplasmic ATPase activity of DIME-treated cells without a decrease in ATP contents of intact cells suggests that DIME may also influence additional as yet unidentified ATP-dependent system(s) probably also involved in cell motility. These results show that DIME not only arrests cells in M phase but also inhibits cell motility in interphase. However the cellular mode of action of DIME is different from the action of other toxic tubulin-targeted drugs, despite the fact that DIME in a concentration-dependent manner disrupts microtubule structures in intact cells.
authors.affiliation: SAN FRANCISCO STATE UNIV,LAB ENVIRONM TOXICOL & CHEM,OCTAMER RES FDN,ROMBERG TIBURON CTR,TIBURON,CA 94920.
authors.forename: K
authors.initials: K
authors.lastname: Buki
authors.forename: E
authors.initials: E
authors.lastname: Kirsten
authors.forename: P
authors.initials: P
authors.lastname: Bauer
authors.forename: C
authors.initials: C
authors.lastname: Vidair
authors.forename: A
authors.initials: A
authors.lastname: Kun
authors.forename: J
authors.initials: J
authors.lastname: Mendeleyev
authors.forename: E
authors.initials: E
authors.lastname: Kun