title: Radiation-induced irreversible g(0) g(1) block is abolished in human-diploid fibroblasts transfected with the human papilloma-virus e6 gene - implication of the p53-cip1 waf1 pathway.
abstract: It has been shown previously with human diploid fibroblasts that a fraction of cells remains irreversibly blocked in the G(0)/G(1) phase of the cell cycle when the cells are irradiated in confluent, density-inhibited cultures and released by subculture to low density. In the present study we demonstrate that this G(0)/G(1) block is abolished when a human fibroblast cell strain is transfected with the human papilloma virus 16 E6 gene. Corresponding to the abolition of the G(0)/G(1) block in the E6-transfected cells, the expression of both the p53 and WAF1 genes is also abolished, suggesting that the p53 pathway of DNA damage response may be involved in this phenomenon.
authors.affiliation: HARVARD UNIV,SCH PUBL HLTH,RADIOBIOL LAB,BOSTON,MA 02115.
authors.forename: C
authors.initials: C
authors.lastname: Li
authors.forename: H
authors.initials: H
authors.lastname: Nagasawa
authors.forename: N
authors.initials: N
authors.lastname: Tsang
authors.forename: J
authors.initials: J
authors.lastname: Little