title: Evaluation of polysaccharide-based chiral stationary phases in quality control of (S)-mirtazapine.
abstract: High-performance liquid chromatographic methods were developed for separation of the enantiomers of mirtazapine and its four process-related substances. The direct separations were achieved on chiral stationary phases containing amylose tris(3,5-dimethylphenylcarbamate) (Chiralpak AD-H), cellulose tris(3,5-dimethylphenylcarbamate) (Chiralcel OD-H) and cellulose tris(4-methylbenzoate) (Chiralcel OJ-H ). The experimental data were utilized to discuss the effects of the mobile phase composition, the nature of the alcoholic modifier and the specific structural features of the analytes on retention and separation. The elution sequence was determined under the optimized separation conditions.
authors.affiliation: HPLC/UV Group, Analytical Chemistry Division, Discovery Laboratory, Indian Institute of Chemical Technology, Tarnaka, Hyderabad 500607, India. rnrao55@yahoo.com
authors.forename: R Nageswara
authors.initials: RN
authors.lastname: Rao
authors.forename: Pawan K
authors.initials: PK
authors.lastname: Maurya
authors.forename: K
authors.initials: K
authors.lastname: Guruprasad
mesh_terms: D000688:Amylose / Q000031:analogs & derivatives* / Q000737:chemistry; D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D008803:Mianserin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000592:standards; D000078785:Mirtazapine; D048448:Phenylcarbamates / Q000737:chemistry*; D013237:Stereoisomerism
chemical_list: D048448:Phenylcarbamates; C070231:Chiracel OJ; C065943:cellulose tris-3,5-dimethylphenyl-carbamate; C080033:Chiralpak AD; D008803:Mianserin; D002482:Cellulose; D000688:Amylose; D000078785:Mirtazapine