title: [The morphological and karyological characteristics of MDCK and vero (B) cells cultures on plant hydrolyzate-based nutrient media].
abstract: MDCK and Vero (B) cell cultures were propagated during 10 passages in the experimental nutrient media containing the soybean powder hydrolyzate prepared using trypsin and bromelain enzymes and the rice powder hydrolysate prepared with trypsin and in the control DMEM and SFM4 MegaVir media. The karyological, morphological, and proliferative characteristics of continuous cultures were examined and compared. The experimental media supplied with 3% fetal bovine serum (FBS) (Gibco, U.S.A.) showed high growth-enhancing properties and failed to affect their morphology. After propagated during 10 passages in the experimental media preserved a stable karyotype. MDCK cell cultures in the nutrient media based on rice and soybean powder hydrolyzates low (2%) in FBS caused no substantial changes in the proliferation indices and morphological and karyological characteristics of cell cultures.
authors.forename: G R
authors.initials: GR
authors.lastname: Mikhailova
authors.forename: N A
authors.initials: NA
authors.lastname: Mazurkova
authors.forename: R Ya
authors.initials: RY
authors.lastname: Podchernyaeva
authors.forename: E I
authors.initials: EI
authors.lastname: Ryabchikova
authors.forename: G P
authors.initials: GP
authors.lastname: Troshkova
authors.forename: L N
authors.initials: LN
authors.lastname: Shishkina
mesh_terms: D000818:Animals; D001963:Bromelains; D002417:Cattle; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D048430:Cell Shape; D002522:Chlorocebus aethiops; D003470:Culture Media; D004285:Dogs; D007621:Karyotyping; D008853:Microscopy; D012275:Oryza; D011492:Protein Hydrolysates; D044967:Serum; D013025:Glycine max; D014357:Trypsin; D014709:Vero Cells
chemical_list: D003470:Culture Media; D011492:Protein Hydrolysates; D001963:Bromelains; D014357:Trypsin