title: Expression of adenosine receptors in human retinal pigment epithelium cells in vitro.
abstract: BACKGROUND
  Adenosine receptors (ADORs) have been reported to play a role in experimental myopia. This study aimed to determine the distribution of ADORs in human retinal pigment epithelium (RPE) cells cultured in vitro.
  
  
  METHODS
  Human RPE cells (cell line D407) were cultured in vitro. ADOR mRNA in RPE was detected by reverse transcription polymerase chain reaction. ADOR protein expression in RPE was confirmed by Western blotting analysis of cell lysates. Confocal fluorescence microscopy was used to study the subcellular distribution of ADORs.
  
  
  RESULTS
  All four subtypes of ADORs mRNA and protein were expressed in human RPE. This was confirmed by Western blotting analysis. The ADOR subtypes were differently distributed within the cells. ADORA1 was expressed in nucleus, perinucleus and cytoplasm of RPE. ADORA2A was concentrated mainly in one side of the perinucleus and cytoplasm of RPE. ADORA2B was strongly expressed in the nucleus, perinucleus and the cytoplasm, and ADORA3 was expressed weakly in the cytoplasm of RPE.
  
  
  CONCLUSIONS
  ADORs are expressed in human RPE. The different distribution at the subcellular level suggests different functions of ADOR subtypes.
authors.affiliation: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, Guangdong, China.
authors.forename: Wen-juan
authors.initials: WJ
authors.lastname: Wan
authors.forename: Dong-mei
authors.initials: DM
authors.lastname: Cui
authors.forename: Xiao
authors.initials: X
authors.lastname: Yang
authors.forename: Jian-min
authors.initials: JM
authors.lastname: Hu
authors.forename: Chuan-xu
authors.initials: CX
authors.lastname: Li
authors.forename: Shou-long
authors.initials: SL
authors.lastname: Hu
authors.forename: Klaus
authors.initials: K
authors.lastname: Trier
authors.forename: Jun-wen
authors.initials: JW
authors.lastname: Zeng
mesh_terms: D015153:Blotting, Western; D002460:Cell Line; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D018047:Receptors, Purinergic P1 / Q000235:genetics / Q000378:metabolism*; D055213:Retinal Pigment Epithelium / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D018047:Receptors, Purinergic P1