title: Synergistic effect of graphene oxide/MWCNT films in laser desorption/ionization mass spectrometry of small molecules and tissue imaging.
abstract: Matrix-assisted laser desorption/ionization mass spectrometry has been considered an important tool for various biochemical analyses and proteomics research. Although addition of conventional matrix efficiently supports laser desorption/ionization of analytes with minimal fragmentation, it often results in high background interference and misinterpretation of the spatial distribution of biomolecules especially in low-mass regions. Here, we show design, systematic characterization, and application of graphene oxide/multiwalled carbon nanotube-based films fabricated on solid substrates as a new matrix-free laser desorption/ionization platform. We demonstrate that the graphene oxide/multiwalled carbon nanotube double layer provides many advantages as a laser desorption/ionization substrate, such as efficient desorption/ionization of analytes with minimum fragmentation, high salt tolerance, no sweet-spots for mass signal, excellent durability against mechanical and photoagitation and prolonged exposure to ambient conditions, and applicability to tissue imaging mass spectrometry. This platform will be widely used as an important tool for mass spectrometry-based biochemical analyses because of its outstanding performance, long-term stability, and cost effectiveness.
authors.affiliation: Department of Chemistry, Institute for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST), 373-1 Guseong Dong, Yuseong-gu, Daejeon 305-701, Korea.
authors.forename: Young-Kwan
authors.initials: YK
authors.lastname: Kim
authors.forename: Hee-Kyung
authors.initials: HK
authors.lastname: Na
authors.forename: Sul-Jin
authors.initials: SJ
authors.lastname: Kwack
authors.forename: Soo-Ryoon
authors.initials: SR
authors.lastname: Ryoo
authors.forename: Youngmi
authors.initials: Y
authors.lastname: Lee
authors.forename: Seunghee
authors.initials: S
authors.lastname: Hong
authors.forename: Sungwoo
authors.initials: S
authors.lastname: Hong
authors.forename: Yong
authors.initials: Y
authors.lastname: Jeong
authors.forename: Dal-Hee
authors.initials: DH
authors.lastname: Min
mesh_terms: D001671:Biochemistry / Q000379:methods; D001826:Body Fluids / Q000737:chemistry; D002475:Cellobiose / Q000737:chemistry; D003952:Diagnostic Imaging / Q000379:methods; D004745:Enkephalins / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D007477:Ions; D007834:Lasers; D008027:Light; D013058:Mass Spectrometry / Q000379:methods; D018625:Microscopy, Atomic Force / Q000379:methods; D036103:Nanotechnology / Q000379:methods*; D037742:Nanotubes, Carbon / Q000737:chemistry; D010087:Oxides / Q000737:chemistry*; D010777:Photochemistry / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D013499:Surface Properties
chemical_list: D004745:Enkephalins; D007477:Ions; D037742:Nanotubes, Carbon; D010087:Oxides; D002475:Cellobiose; D006108:Graphite