title: Development of an HPLC method for the determination of anidulafungin in human plasma and saline.
abstract: An ultraviolet high-performance liquid chromatography (HPLC) method was developed to analyze anidulafungin in human plasma and saline. A reversed-phase column was used with a UV detector set at 310 nm. The mobile phase consisted of methanol and ammonium phosphate buffer at a flow rate of 1 mL/min. Micafungin was used as the internal standard. Both standard curves were linear over a range of 1 to 10 μg/mL. The intra-assay relative standard deviations (RSD) for plasma and saline matrices were 1.60-1.81% and 1.96-3.70%, respectively. The inter-assay RSD for plasma and saline matrices were 2.41-7.25% and 1.31-3.16%, respectively. This method successfully recapitulated anidulafungin plasma concentrations previously analyzed by HPLC-tandem mass spectrometry with precision and accuracy of 6.9% and 1.59%, respectively.
authors.affiliation: Center for Anti-Infective Research and Development, Hartford Hospital, Hartford, CT 06102, USA.
authors.forename: Christina A
authors.initials: CA
authors.lastname: Sutherland
authors.forename: David P
authors.initials: DP
authors.lastname: Nicolau
authors.forename: Joseph L
authors.initials: JL
authors.lastname: Kuti
mesh_terms: D000077612:Anidulafungin; D000935:Antifungal Agents / Q000032:analysis / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D054714:Echinocandins / Q000032:analysis* / Q000097:blood*; D006801:Humans; D016018:Least-Squares Analysis; D055666:Lipopeptides; D000077551:Micafungin; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012965:Sodium Chloride / Q000737:chemistry; D053719:Tandem Mass Spectrometry
chemical_list: D000935:Antifungal Agents; D054714:Echinocandins; D055666:Lipopeptides; D012965:Sodium Chloride; D000077612:Anidulafungin; D000077551:Micafungin