title: Well-controlled cationic water-soluble phospholipid polymer-DNA nanocomplexes for gene delivery.
abstract: The facile synthesis of biocompatible and nontoxic gene delivery vectors has been the focus of research in recent years due to the high potential in treating genetic diseases. 2-Methacryloxyethyl phosphorylcholine (MPC) copolymers were recently studied for their ability to produce nontoxic and biocompatible materials. The synthesis of well-defined and water-soluble MPC polymer based cationic vectors for gene delivery purposes was therefore attractive, due to the potential excellent biocompatibility of the resulting copolymers. Herein, cationic MPC copolymers of varying architectures (block versus random) were produced by the reversible addition--fragmentation chain transfer (RAFT) polymerization technique. The copolymers produced were evaluated for their gene delivery efficacy in the presence and absence of serum. It was found that copolymer architectures and molecular weights do affect their gene delivery efficacy. The statistical copolymers produced larger particles, and showed poor gene transfection efficiency as compared to the diblock copolymers. The diblock copolymers served as efficient gene delivery vectors, in both the presence and absence of serum in vitro. To the best of our knowledge, this is the first report where the effect of architecture of MPC based copolymer on gene delivery efficacy has been studied.
authors.affiliation: Department of Chemical and Materials Engineering, Alberta Ingenuity Centre for Carbohydrate Science, University of Alberta, Edmonton, AB, T6G 2G6, Canada.
authors.forename: Marya
authors.initials: M
authors.lastname: Ahmed
authors.forename: Neha
authors.initials: N
authors.lastname: Bhuchar
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Ishihara
authors.forename: Ravin
authors.initials: R
authors.lastname: Narain
mesh_terms: D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D002412:Cations / Q000737:chemistry; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000737:chemistry*; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000138:chemical synthesis / Q000737:chemistry; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D008689:Methacrylates / Q000737:chemistry*; D015394:Molecular Structure; D049329:Nanostructures / Q000737:chemistry*; D010316:Particle Size; D010767:Phosphorylcholine / Q000031:analogs & derivatives* / Q000737:chemistry; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D012995:Solubility; D013237:Stereoisomerism; D013499:Surface Properties; D014018:Tissue Distribution; D014867:Water / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D002412:Cations; D008689:Methacrylates; D011108:Polymers; D014867:Water; D010767:Phosphorylcholine; C070638:2-methacryloyloxyethyl phosphorylcholine; D004247:DNA