title: Renal inflammation and elevated blood pressure in a mouse model of reduced {beta}-ENaC.
abstract: Previous studies suggest β-epithelial Na(+) channel protein (β-ENaC) may mediate myogenic constriction, a mechanism of blood flow autoregulation. A recent study demonstrated that mice with reduced levels of β-ENaC (β-ENaC m/m) have delayed correction of whole kidney blood flow responses, suggesting defective myogenic autoregulatory capacity. Reduced renal autoregulatory capacity is linked to renal inflammation, injury, and hypertension. However, it is unknown whether β-ENaC m/m mice have any complications associated with reductions in autoregulatory capacity such as renal inflammation, injury, or hypertension. To determine whether the previously observed altered autoregulatory control was associated with indicators of renal injury, we evaluated β-ENaC m/m mice for signs of renal inflammation and tissue remodeling using marker expression. We found that inflammatory and remodeling markers, such as IL-1β, IL-6, TNF-α, collagen III and transforming growth factor-β, were significantly upregulated in β-ENaC m/m mice. To determine whether renal changes were associated with changes in long-term control of blood pressure, we used radiotelemetry and found that 5-day mean arterial blood pressure (MAP) was significantly elevated in β-ENaC m/m (120 ± 3 vs. 105 ± 2 mmHg, P = 0.016). Our findings suggest loss of β-ENaC is associated with early signs of renal injury and increased MAP.
authors.affiliation: Dept. of Physiology and Biophysics, Univ. of Mississippi Medical Center, Jackson, 39216-4505, USA. hdrummond@umc.edu
authors.forename: Heather A
authors.initials: HA
authors.lastname: Drummond
authors.forename: Samira C
authors.initials: SC
authors.lastname: Grifoni
authors.forename: Ahmed
authors.initials: A
authors.lastname: Abu-Zaid
authors.forename: Monette
authors.initials: M
authors.lastname: Gousset
authors.forename: Rumbidayzi
authors.initials: R
authors.lastname: Chiposi
authors.forename: John M
authors.initials: JM
authors.lastname: Barnard
authors.forename: Beau
authors.initials: B
authors.lastname: Murphey
authors.forename: David E
authors.initials: DE
authors.lastname: Stec
mesh_terms: D000818:Animals; D001794:Blood Pressure; D053503:Epithelial Sodium Channels / Q000378:metabolism*; D005260:Female; D006339:Heart Rate; D006706:Homeostasis; D006977:Hypertension, Renal / Q000209:etiology* / Q000378:metabolism; D008297:Male; D051379:Mice; D009043:Motor Activity; D009131:Muscle, Smooth, Vascular / Q000502:physiology*; D009393:Nephritis / Q000209:etiology* / Q000378:metabolism; D012079:Renal Circulation
chemical_list: D053503:Epithelial Sodium Channels