title: Effect of xylanase and glucanase supplementation to a cereal-based, threonine-limited diet on the nitrogen balance of growing pigs.
abstract: In cereal-based diets, non-starch polysaccharides (NSP) lower precaecal nutrient absorption and increase endogenous protein and amino acid (AA) losses. Adding exogenous NSP-degrading enzymes aims amongst others to reduce these negative effects and to thereby improve protein and AA supply. However, biased results exist in the literature on their efficacy in growing pigs. Hence, the objective of this study was to analyse the effects of different levels of xylanase and beta-glucanase supplementation. Nitrogen (N) retention from a threonine-limited diet was chosen as an indirect indicator for differences in praecaecal threonine absorption and endogenous protein and AA losses. During three balance periods, 12 male pigs with a bodyweight of 31-66 kg were used in a cross-over design. They received three different diets based on wheat, barley, rye, and soybean meal containing 0, 40 or 80 mg/kg of an enzyme preparation containing endo-1,4,-beta-xylanase and endo-1,4-beta-glucanase. N excretion and retention were identical in animals of the different treatment groups, stressing that enzyme supplementation did not affect threonine absorption and/or endogenous protein and AA losses neither at medium nor at high supplementation level. Hence, in the present trial, beta-glucanase and xylanase addition to cereal diets did not improve protein and AA availability in growing pigs of a body weight > 30 kg.
authors.affiliation: "Institute of Animal Nutrition and Physiology, Christian-Albrechts-University, Kiel, Germany. susenbeth@aninut.uni-kiel.de
authors.forename: Andreas
authors.initials: A
authors.lastname: Susenbeth
authors.forename: Maike
authors.initials: M
authors.lastname: Naatjes
authors.forename: Britta
authors.initials: B
authors.lastname: Blank
authors.forename: Rebecca
authors.initials: R
authors.lastname: Kühl
authors.forename: Peter
authors.initials: P
authors.lastname: Ader
authors.forename: Uta
authors.initials: U
authors.lastname: Dickhoefer
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D004032:Diet / Q000662:veterinary*; D019587:Dietary Supplements; D002523:Edible Grain; D043364:Endo-1,4-beta Xylanases / Q000494:pharmacology*; D006026:Glycoside Hydrolases / Q000494:pharmacology*; D008297:Male; D009584:Nitrogen / Q000378:metabolism; D013552:Swine / Q000254:growth & development* / Q000378:metabolism*; D013912:Threonine / Q000172:deficiency*
chemical_list: D013912:Threonine; D006026:Glycoside Hydrolases; D043364:Endo-1,4-beta Xylanases; D009584:Nitrogen