title: BMPR-II is dispensable for formation of the limb skeleton.
abstract: Initiation of BMP signaling is dependent upon activation of Type I BMP receptor by constitutively active Type II BMP receptor. Three Type II BMP receptors have been identified; Acvr2a and Acvr2b serve as receptors for BMPs and for activin-like ligands whereas BMPR-II functions only as a BMP receptor. As BMP signaling is required for endochondral ossification and loss of either Acvr2a or Acvr2b is not associated with deficits in limb development, we hypothesized that BMPR-II would be essential for BMP signaling during skeletogenesis. We removed BMPR-II from early limb mesoderm by crossing BMPR-II floxed mice with those carrying the Prx1-Cre transgene. Mice lacking limb expression of BMPR-II have normal skeletons that could not be distinguished from control littermates. From these data, we conclude that BMPR-II is not required for endochondral ossification in the limb where loss of BMPR-II may be compensated by BMP utilization of Acvr2a and Acvr2b.
authors.forename: Laura W
authors.initials: LW
authors.lastname: Gamer
authors.forename: Kunikazu
authors.initials: K
authors.lastname: Tsuji
authors.forename: Karen
authors.initials: K
authors.lastname: Cox
authors.forename: Luciane P
authors.initials: LP
authors.lastname: Capelo
authors.forename: Jonathan
authors.initials: J
authors.lastname: Lowery
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Beppu
authors.forename: Vicki
authors.initials: V
authors.lastname: Rosen
mesh_terms: D030301:Activin Receptors, Type II / Q000235:genetics / Q000378:metabolism; D000818:Animals; D052005:Bone Morphogenetic Protein Receptors, Type I / Q000235:genetics / Q000378:metabolism*; D052006:Bone Morphogenetic Protein Receptors, Type II / Q000235:genetics / Q000378:metabolism*; D019485:Bone Morphogenetic Proteins / Q000235:genetics / Q000378:metabolism*; D005121:Extremities / Q000196:embryology*; D018507:Gene Expression Regulation, Developmental; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D010012:Osteogenesis / Q000235:genetics*; D010641:Phenotype; D011485:Protein Binding; D017384:Sequence Deletion; D015398:Signal Transduction / Q000235:genetics; D019076:Transgenes
chemical_list: D019485:Bone Morphogenetic Proteins; D030301:Activin Receptors, Type II; C498219:Bmpr2 protein, mouse; D052005:Bone Morphogenetic Protein Receptors, Type I; D052006:Bone Morphogenetic Protein Receptors, Type II; C425585:activin receptor type II-A; C425584:activin receptor type II-B