title: Micro- and nanoengineering for stem cell biology: the promise with a caution.
abstract: Current techniques used in stem cell research only crudely mimic the physiological complexity of the stem cell niches. Recent advances in the field of micro- and nanoengineering have brought an array of in vitro cell culture models that have enabled development of novel, highly precise and standardized tools that capture physiological details in a single platform, with greater control, consistency, and throughput. In this review, we describe the micro- and nanotechnology-driven modern toolkit for stem cell biologists to design novel experiments in more physiological microenvironments with increased precision and standardization, and caution them against potential challenges that the modern technologies might present.
authors.affiliation: Department of Biomedical Engineering, The Johns Hopkins Medical Institutions, Baltimore, MD, USA.
authors.lastname: Kshitiz
authors.forename: Deok-Ho
authors.initials: DH
authors.lastname: Kim
authors.forename: David J
authors.initials: DJ
authors.lastname: Beebe
authors.forename: Andre
authors.initials: A
authors.lastname: Levchenko
mesh_terms: D000818:Animals; D057005:Bioengineering / Q000379:methods*; D006801:Humans; D057905:Stem Cell Research; D013234:Stem Cells / Q000166:cytology*