title: 2D-immunoblotting analysis of Sporothrix schenckii cell wall.
abstract: We utilized two-dimensional gel electrophoresis and immunoblotting (2D-immunoblotting) with anti-Sporothrix schenckii antibodies to identify antigenic proteins in cell wall preparations obtained from the mycelial and yeast-like morphologies of the fungus. Results showed that a 70-kDa glycoprotein (Gp70) was the major antigen detected in the cell wall of both morphologies and that a 60-kDa glycoprotein was present only in yeast-like cells. In addition to the Gp70, the wall from filament cells showed four proteins with molecular weights of 48, 55, 66 and 67 kDa, some of which exhibited several isoforms. To our knowledge, this is the first 2D-immunoblotting analysis of the S. schenckii cell wall.
authors.affiliation: Facultad de Ciencias Químicas, Universidad Juárez del Estado de Durango, Durango, México. erb750@hotmail.com
authors.forename: Estela
authors.initials: E
authors.lastname: Ruiz-Baca
authors.forename: Héctor M
authors.initials: HM
authors.lastname: Mora-Montes
authors.forename: Everardo
authors.initials: E
authors.lastname: López-Romero
authors.forename: Conchita
authors.initials: C
authors.lastname: Toriello
authors.forename: Virgilio
authors.initials: V
authors.lastname: Mojica-Marín
authors.forename: Norma
authors.initials: N
authors.lastname: Urtiz-Estrada
mesh_terms: D000818:Animals; D000946:Antigens, Fungal / Q000032:analysis*; D002473:Cell Wall / Q000276:immunology*; D015180:Electrophoresis, Gel, Two-Dimensional; D015151:Immunoblotting; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis*; D011817:Rabbits; D013175:Sporothrix / Q000276:immunology* / Q000302:isolation & purification
chemical_list: D000946:Antigens, Fungal; D008562:Membrane Glycoproteins