title: Colorimetric artificial tongue for protein identification.
abstract: Artificial tongue systems are multisensory devices which are highly desirable for the analysis and recognition of complicated composition samples. Herein, a low-cost and simple colorimetric sensor array for identification and quantification of proteins were reported. Using prophyrin, porphyrin derivatives (mainly metalloporphyrins) and chemically responsive dyes as the sensing elements, the developed sensor array of artificial tongue showed a unique pattern of colorific change upon its exposure to proteins. The composite pattern for each sample was subjected to principal component analysis (PCA), thus providing a clustering map for more practical visualization. All the pure and mixed proteins, as well as denatured proteins, gave distinct patterns, thus resulting in their unambiguous identification. The PCA analysis also suggested that the unique pattern of colorific change may be due to the change of protein conformation and local environmental pH. These results demonstrate that the developed colorimetric artificial tongue system is an excellent sensing platform for identification and quantitative analysis of protein samples.
authors.affiliation: Key Laboratory of Biorheology Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, 400044, PR China.
authors.forename: Changjun
authors.initials: C
authors.lastname: Hou
authors.forename: Jiale
authors.initials: J
authors.lastname: Dong
authors.forename: Guoping
authors.initials: G
authors.lastname: Zhang
authors.forename: Yu
authors.initials: Y
authors.lastname: Lei
authors.forename: Mei
authors.initials: M
authors.lastname: Yang
authors.forename: Yuchan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Liu
authors.forename: Suyi
authors.initials: S
authors.lastname: Zhang
authors.forename: Danqun
authors.initials: D
authors.lastname: Huo
mesh_terms: D001187:Artificial Organs; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods / Q000706:statistics & numerical data; D003124:Colorimetry / Q000295:instrumentation / Q000379:methods; D004396:Coloring Agents; D006863:Hydrogen-Ion Concentration; D011166:Porphyrins; D025341:Principal Component Analysis; D011487:Protein Conformation; D011489:Protein Denaturation; D011506:Proteins / Q000032:analysis*; D014059:Tongue
chemical_list: D004396:Coloring Agents; D011166:Porphyrins; D011506:Proteins