title: BTI1, an azoreductase with pH-dependent substrate specificity.
abstract: The group II azoreductase BTI1 utilizes NADPH to directly cleave azo bonds in water-soluble azo dyes, including quenchers of fluorescence. Unexpectedly, optimal reduction was dye specific, ranging from a pH of <5.5 for Janus green B, to pH 6.0 for methyl red, methyl orange, and BHQ-10, to pH >8.3 for flame orange.
authors.affiliation: Biosearch Technologies, Inc., 81 Digital Drive, Novato, CA 94949-5728, USA. hjohansson@biosearchtech.com
authors.forename: Hans E
authors.initials: HE
authors.lastname: Johansson
authors.forename: Mary K
authors.initials: MK
authors.lastname: Johansson
authors.forename: Albert C
authors.initials: AC
authors.lastname: Wong
authors.forename: Eliana S
authors.initials: ES
authors.lastname: Armstrong
authors.forename: Erik J
authors.initials: EJ
authors.lastname: Peterson
authors.forename: Richard E
authors.initials: RE
authors.lastname: Grant
authors.forename: Margaret A
authors.initials: MA
authors.lastname: Roy
authors.forename: Mark V
authors.initials: MV
authors.lastname: Reddington
authors.forename: Ronald M
authors.initials: RM
authors.lastname: Cook
mesh_terms: D001391:Azo Compounds / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D009249:NADP / Q000378:metabolism*; D009601:Nitroreductases; D013379:Substrate Specificity
chemical_list: D001391:Azo Compounds; D009249:NADP; D009247:NADH, NADPH Oxidoreductases; D009601:Nitroreductases; C021106:azoreductase