title: [Simultaneous determination of sixteen antibiotics in human urine with ultra performance liquid chromatography-tandem mass spectrometry].
abstract: OBJECTIVE
  To develop a method for simultaneous determination of sixteen antibiotics in human urine by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).
  
  
  METHODS
  With Piperacillin as an internal standard, the target antibiotics in urine samples were enriched and purified by Oasis HLB solid phase extraction (SPE) cartridges, then separated in a ZORBAX SB-C18 column with a gradient elution of mobile phase of 0.1% formic acid water and acetonitrile, finally analyzed with multiple reaction monitoring (MRM) mode.
  
  
  RESULTS
  The limits of detection (LOD) for these sixteen antibiotics were in the range of 0.05-10.0 ng/mL and the limits of quantification (LOQ) in the range of 0.25-20.0 ng/mL. Within the related linear range, the related coefficient (r) of sixteen antibiotics were all more than 0.995. Accuracies for these antibiotics were ranged from 82.0%-119.3%, the within-day precision were less than 13.9%.
  
  
  CONCLUSIONS
  The developed method is sensitive, specific and appropriate for the analysis of antibiotics in forensic toxicology and therapeutic drugs monitoring.
authors.affiliation: Department of Forensic Medicine, Shanghai Medical College of Fudan University, Shanghai 200032, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Chen
authors.forename: Hui
authors.initials: H
authors.lastname: Yan
authors.forename: Bao-hua
authors.initials: BH
authors.lastname: Shen
authors.forename: Xian-yi
authors.initials: XY
authors.lastname: Zhuo
mesh_terms: D000667:Ampicillin / Q000652:urine; D000900:Anti-Bacterial Agents / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D053593:Forensic Toxicology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010406:Penicillins / Q000652:urine; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction; D012997:Solvents / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D010406:Penicillins; D012997:Solvents; D000667:Ampicillin