title: Characterizing the proteome of the human dorsolateral prefrontal cortex by shotgun mass spectrometry.
abstract: The studies of neuropsychiatric disorders would be facilitated by enhanced knowledge of the dorsolateral prefrontal cortex (DLPFC) proteome. To construct a data set of human DLPFC proteins, protein extracts were prepared from 12 postmortem brains focussing on the DLPFC region (Brodmann area 9) and analyzed using a combined gel electrophoresis and shotgun mass spectrometry approach, featuring data-independent label-free nanoflow liquid chromatography mass spectrometry (nLC-MS(E)). The detected mass/time features were aligned and annotated using the results from ProteinLynx Global Server. The resulting data set comprised 488 unique and accurately identified proteins, with stringent identification by a minimum of two distinct peptides detected at least in >75% of samples. These proteins were involved predominantly in cytoskeletal architecture, metabolism, transcription/translation, and synaptic function. Combination of this data set with that obtained by our previous characterization of the same brain region results in a total of 755 unique proteins, making this the most comprehensive analysis of this important brain region to date.
authors.affiliation: Institute of Biotechnology, University of Cambridge, Cambridge, UK. dm533@cam.ac.uk
authors.forename: Daniel
authors.initials: D
authors.lastname: Martins-de-Souza
authors.forename: Paul C
authors.initials: PC
authors.lastname: Guest
authors.forename: Hannah
authors.initials: H
authors.lastname: Steeb
authors.forename: Sandra
authors.initials: S
authors.lastname: Pietsch
authors.forename: Hassan
authors.initials: H
authors.lastname: Rahmoune
authors.forename: Laura W
authors.initials: LW
authors.lastname: Harris
authors.forename: Sabine
authors.initials: S
authors.lastname: Bahn
mesh_terms: D030562:Databases, Protein; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D009419:Nerve Tissue Proteins / Q000032:analysis* / Q000737:chemistry / Q000145:classification; D010449:Peptide Mapping / Q000379:methods*; D017397:Prefrontal Cortex / Q000737:chemistry*; D020543:Proteome / Q000032:analysis* / Q000737:chemistry; D040901:Proteomics / Q000379:methods*
chemical_list: D009419:Nerve Tissue Proteins; D020543:Proteome