title: Analyte-induced formation of partial duplexes for the preparation of a label-free electrochemiluminescent aptasensor.
abstract: Analyte-induced formation of partial duplexes was used for biosensor development with cocaine as a model. The cocaine-aptamer interaction resulted in formation of a partial double strand section in the aptamer, where Ru(phen)(3)(2+) was intercalated for electrochemiluminescent analysis of cocaine.
authors.affiliation: Research Center for Analytical Sciences, College of Chemistry, Nankai University, Tianjin, 300071, PR. China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhao
authors.forename: Xi-Wen
authors.initials: XW
authors.lastname: He
authors.forename: Xue-Bo
authors.initials: XB
authors.lastname: Yin
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D003042:Cocaine / Q000032:analysis*; D056831:Coordination Complexes / Q000737:chemistry; D004566:Electrodes; D006046:Gold / Q000737:chemistry; D007364:Intercalating Agents / Q000737:chemistry; D008163:Luminescent Measurements / Q000379:methods*; D012428:Ruthenium / Q000737:chemistry
chemical_list: D052157:Aptamers, Nucleotide; D056831:Coordination Complexes; D007364:Intercalating Agents; D006046:Gold; D012428:Ruthenium; D003042:Cocaine