title: Enzyme-immobilized CNT network probe for in vivo neurotransmitter detection.
abstract: Glutamate is the principal excitatory neurotransmitter in the brain, and its excessive release plays a key role in neuronal death associated with a wide range of neural disorders. Real-time monitoring of extracellular glutamate levels would be very helpful in understanding the excitotoxic process of neurotransmitters on brain injury. Toward the detection of L: -glutamate, we describe in this chapter the preparation of carbon nanotube (CNT) network probes with immobilized L: -glutamate oxidase (GLOD) by using a non-covalent functionalized method. Such GOLD-CNT network probes are evaluated with real-time electronic responses corresponding to standard glutamate solutions in vitro and a 11-vessel occlusion (11 VO) rat model in vivo. The ultrahigh sensitivity, selectivity, and fast response time of GLOD-CNT network probes are greatly promising for the real-time electronic detection of extracellular glutamate levels in brain.
authors.affiliation: Department of Biomedical Engineering, College of Medicine, Kyung Hee University, Seoul 130-701, Republic of Korea.
authors.forename: Gi-Ja
authors.initials: GJ
authors.lastname: Lee
authors.forename: Seok Keun
authors.initials: SK
authors.lastname: Choi
authors.forename: Samjin
authors.initials: S
authors.lastname: Choi
authors.forename: Ji Hye
authors.initials: JH
authors.lastname: Park
authors.forename: Hun-Kuk
authors.initials: HK
authors.lastname: Park
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D015374:Biosensing Techniques; D001921:Brain / Q000502:physiology; D004195:Disease Models, Animal; D004569:Electroencephalography; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D005110:Extracellular Space / Q000378:metabolism; D018698:Glutamic Acid / Q000032:analysis / Q000096:biosynthesis; D008297:Male; D037742:Nanotubes, Carbon / Q000737:chemistry*; D018377:Neurotransmitter Agents / Q000032:analysis* / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D020521:Stroke / Q000378:metabolism / Q000503:physiopathology; D009435:Synaptic Transmission
chemical_list: D004800:Enzymes, Immobilized; D037742:Nanotubes, Carbon; D018377:Neurotransmitter Agents; D018698:Glutamic Acid; D000594:Amino Acid Oxidoreductases; C023061:L-glutamate oxidase