title: Evaluation of bacteriochlorophyll-reconstituted low-density lipoprotein nanoparticles for photodynamic therapy efficacy in vivo.
abstract: OBJECTIVE
  To evaluate the novel nanoparticle reconstituted bacteriochlorin e6 bisoleate low-density lipoprotein (r-Bchl-BOA-LDL) for its efficacy as a photodynamic therapy agent delivery system in xenografts of human hepatoblastoma G2 (HepG2) tumors.
  
  
  METHODS
  Bchl-BOA was encapsulated in the nanoparticle low-density lipoprotein (LDL), a native particle whose receptor's overexpression is a cancer signature for a number of neoplasms. Evaluation of r-Bchl-BOA-LDL as a potential photosensitizer was performed using a tumor response and foot response assay.
  
  
  CONCLUSIONS
  When compared with controls, tumor regrowth was significantly delayed at injected murine doses of 2 µmole/kg r-Bchl-BOA-LDL after illumination at fluences of 125, 150 or 175 J/cm(2). Foot response assays showed that although normal tissue toxicity accompanied the higher fluences it was significantly reduced at the lowest fluence tested.
  
  
  CONCLUSIONS
  This research demonstrates that r-Bchl-BOA-LDL is an effective photosensitizer and a promising candidate for further investigation.
authors.affiliation: Department of Radiation Oncology, School of Medicine, University of Pennsylvania, PA 19104, USA. dmarott1@jhu.edu
authors.forename: Diane E
authors.initials: DE
authors.lastname: Marotta
authors.forename: Weiguo
authors.initials: W
authors.lastname: Cao
authors.forename: E Paul
authors.initials: EP
authors.lastname: Wileyto
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Ian
authors.initials: I
authors.lastname: Corbin
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Rickter
authors.forename: Jerry D
authors.initials: JD
authors.lastname: Glickson
authors.forename: Britton
authors.initials: B
authors.lastname: Chance
authors.forename: Gang
authors.initials: G
authors.lastname: Zheng
authors.forename: Theresa M
authors.initials: TM
authors.lastname: Busch
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D016503:Drug Delivery Systems; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D009369:Neoplasms / Q000188:drug therapy*; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000008:administration & dosage*; D023041:Xenograft Model Antitumor Assays
chemical_list: D017319:Photosensitizing Agents