title: [Study on purification of polysaccharides in root of Salvia miltiorrhiza by macroporous adsorption resin and ion-exchange resin methods].
abstract: OBJECTIVE
  To study the purification and isolation of polysaccharides from Salvia miltiorrhiza.
  
  
  METHODS
  The root was extracted by water purified preliminarily by alcohol precipitation, and then four different types macroporous adsorption resin and one ion-exchange resin were comparatively investigated in the purification and isolation of Salvia polysaccharides.
  
  
  RESULTS
  The total polysaccharides of crude extracts was 40.35%, and protein content amounted to 8.96%. Compared with the traditional methods, AB-8, DB-301 type of resin used in purification of polysaccharides could simplify the working process and obtain better effect. Then the obtained crude polysaccharides through AB-8 resin were purified by ion-exchange resin DEAE-52. Three portions of powder were obtained through lyophilization and named as SMP1, SMP2, SMP3.
  
  
  CONCLUSIONS
  Purification of Salvia polysaccharides can be conducted by adoping AB-8, DB-301 type of resin and DEAE-52 ion-exchange resin.
authors.affiliation: Department of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China. tangwei0516@163.com
authors.forename: Wei
authors.initials: W
authors.lastname: Tang
authors.forename: Qiu-Xian
authors.initials: QX
authors.lastname: Peng
authors.forename: Yu-Miao
authors.initials: YM
authors.lastname: Yan
authors.forename: Zhi-Xian
authors.initials: ZX
authors.lastname: Mo
authors.forename: Shao-Hui
authors.initials: SH
authors.lastname: Huang
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.forename: Hong-Bing
authors.initials: HB
authors.lastname: Cai
mesh_terms: D000327:Adsorption; D002852:Chromatography, Ion Exchange; D000431:Ethanol; D007475:Ion Exchange Resins; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011134:Polysaccharides / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D011506:Proteins / Q000302:isolation & purification; D012117:Resins, Synthetic; D027343:Rhizome / Q000737:chemistry; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D013678:Technology, Pharmaceutical / Q000379:methods
chemical_list: D007475:Ion Exchange Resins; D011134:Polysaccharides; D011506:Proteins; D012117:Resins, Synthetic; D000431:Ethanol