title: Induction of type B synoviocyte-like cells from plasmacytoid dendritic cells of the bone marrow in rheumatoid arthritis and osteoarthritis.
abstract: We examined the capacities of bone marrow (BM) plasmacytoid dendritic cells (pDC) to differentiate into type B synoviocyte-like cells. BM aspiration samples were obtained from 24 rheumatoid arthritis (RA) patients and 19 osteoarthritis (OA) patients during joint operations from the iliac crest. CD34+ cells and pDC purified from BM mononuclear cells were cultured with or without SCF, GM-CSF, and TNF-α for 2-4 weeks. RA BM pDC as well as OA BM pDC comparably differentiated into fibroblast-like cells (FLC), expressing cadherin-11 and producing MMP-1, especially in the presence of TNF-α. Of note, depletion of BDCA4+ pDC from RA BM CD34+ cells significantly diminished their capacities to differentiate into FLC, which were restored by addition of BDCA4+cells in a dose-response manner. These results indicate that pDC is one of the progenitors of type B synoviocytes, suggesting that BM pDC might be involved in the pathogenesis of RA and OA.
authors.affiliation: Department of Rheumatology and Infectious Diseases, Kitasato University School of Medicine, Kanagawa 252-0374, Japan.
authors.forename: Shunsei
authors.initials: S
authors.lastname: Hirohata
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Nagai
authors.forename: Kurumi
authors.initials: K
authors.lastname: Asako
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Tomita
authors.forename: Hideki
authors.initials: H
authors.lastname: Yoshikawa
mesh_terms: D018952:Antigens, CD34 / Q000276:immunology; D000954:Antigens, Surface; D018501:Antirheumatic Agents / Q000627:therapeutic use; D001172:Arthritis, Rheumatoid / Q000188:drug therapy / Q000276:immunology*; D001853:Bone Marrow / Q000276:immunology*; D015820:Cadherins / Q000096:biosynthesis / Q000276:immunology; D002454:Cell Differentiation / Q000276:immunology; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000276:immunology; D006801:Humans; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000096:biosynthesis; D008875:Middle Aged; D010003:Osteoarthritis / Q000276:immunology*; D019089:Stem Cell Factor / Q000276:immunology; D013583:Synovial Membrane / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
chemical_list: D018952:Antigens, CD34; D000954:Antigens, Surface; D018501:Antirheumatic Agents; D015820:Cadherins; D019089:Stem Cell Factor; D014409:Tumor Necrosis Factor-alpha; C478196:blood dendritic cell antigen 4, human; C086960:osteoblast cadherin; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D020781:Matrix Metalloproteinase 1