title: Fasting and high-fat diet alter histone deacetylase expression in the medial hypothalamus.
abstract: Increasing attention is now being given to the epigenetic regulation of animal and human behaviors including the stress response and drug addiction. Epigenetic factors also influence feeding behavior and metabolic phenotypes, such as obesity and insulin sensitivity. In response to fasting and high-fat diets, the medial hypothalamus changes the expression of neuropeptides regulating feeding, metabolism, and reproductive behaviors. Histone deacetylases (HDACs) are involved in the epigenetic control of gene expression and alter behavior in response to a variety of environmental factors. Here, we examined the expression of HDAC family members in the medial hypothalamus of mice in response to either fasting or a high-fat diet. In response to fasting, HDAC3 and -4 expression levels increased while HDAC10 and -11 levels decreased. Four weeks on a high-fat diet resulted in the increased expression of HDAC5 and -8. Moreover, fasting decreased the number of acetylated histone H3- and acetylated histone H4-positive cells in the ventrolateral subdivision of the ventromedial hypothalamus. Therefore, HDACs may be implicated in altered gene expression profiles in the medial hypothalamus under different metabolic states.
authors.affiliation: Department of Anatomy, Toho University School of Medicine, Tokyo, Japan. hiromasa.funato@med.toho-u.ac.jp
authors.forename: Hiromasa
authors.initials: H
authors.lastname: Funato
authors.forename: Satoko
authors.initials: S
authors.lastname: Oda
authors.forename: Junko
authors.initials: J
authors.lastname: Yokofujita
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Igarashi
authors.forename: Masaru
authors.initials: M
authors.lastname: Kuroda
mesh_terms: D000107:Acetylation / Q000187:drug effects; D000818:Animals; D001111:Arcuate Nucleus of Hypothalamus / Q000187:drug effects / Q000201:enzymology; D004041:Dietary Fats / Q000008:administration & dosage / Q000494:pharmacology*; D005215:Fasting / Q000502:physiology*; D005247:Feeding Behavior / Q000187:drug effects; D006655:Histone Deacetylases / Q000378:metabolism*; D006657:Histones / Q000378:metabolism; D006801:Humans; D007031:Hypothalamus / Q000187:drug effects* / Q000201:enzymology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000187:drug effects / Q000201:enzymology; D011333:Pro-Opiomelanocortin / Q000378:metabolism
chemical_list: D004041:Dietary Fats; D006657:Histones; D011333:Pro-Opiomelanocortin; D006655:Histone Deacetylases