title: Is anti-Müllerian hormone a marker of acute cyclophosphamide-induced ovarian follicular destruction in mice pretreated with cetrorelix?
abstract: OBJECTIVE
  To define whether anti-Müllerian hormone (AMH) may be a marker of acute cyclophosphamide (CTX)-induced germ cell destruction in mice pretreated with the GnRH antagonist, cetrorelix.
  
  
  METHODS
  Controlled, experimental study.
  
  
  METHODS
  Research laboratory in a federal research facility.
  
  
  METHODS
  Balb/c female mice (6 weeks old).
  
  
  METHODS
  Mice were treated with GnRH antagonist (cetrorelix) or saline for 15 days followed by 75 mg/kg or 100 mg/kg of CTX or saline control on day 9.
  
  
  METHODS
  Number of primordial follicles (PMF), DNA damage, AMH protein expression, and AMH serum levels.
  
  
  RESULTS
  Ovaries in mice pretreated with cetrorelix had significantly more PMFs and reduced DNA damage compared with those exposed to CTX alone. Immunohistochemical staining for AMH expression and serum AMH levels did not differ significantly between treatment groups.
  
  
  CONCLUSIONS
  Cetrorelix protected PMFs and reduced DNA damage in follicles of mice treated with CTX, but AMH levels in tissue and serum did not correlate with germ cell destruction. Further research is needed to determine the mechanism responsible for the protective effects on PMF counts observed with cetrorelix.
authors.affiliation: Program in Reproductive and Adult Endocrinology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: Hyacinth N
authors.initials: HN
authors.lastname: Browne
authors.forename: Kimberly S
authors.initials: KS
authors.lastname: Moon
authors.forename: Sunni L
authors.initials: SL
authors.lastname: Mumford
authors.forename: Enrique F
authors.initials: EF
authors.lastname: Schisterman
authors.forename: Alan H
authors.initials: AH
authors.lastname: Decherney
authors.forename: James H
authors.initials: JH
authors.lastname: Segars
authors.forename: Alicia Y
authors.initials: AY
authors.lastname: Armstrong
mesh_terms: D000818:Animals; D054304:Anti-Mullerian Hormone / Q000097:blood*; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D015415:Biomarkers / Q000097:blood; D003520:Cyclophosphamide / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000008:administration & dosage / Q000031:analogs & derivatives*; D006727:Hormone Antagonists / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D006080:Ovarian Follicle / Q000187:drug effects* / Q000473:pathology*; D011897:Random Allocation
chemical_list: D015415:Biomarkers; D006727:Hormone Antagonists; D007987:Gonadotropin-Releasing Hormone; D054304:Anti-Mullerian Hormone; D003520:Cyclophosphamide; C062876:cetrorelix