title: Photoluminescent hyperbranched poly(amido amine) containing β-cyclodextrin as a nonviral gene delivery vector.
abstract: Hyperbranched poly(amido amine)s (HPAAs) containing different amounts of β-cyclodextrin (β-CD) (HPAA-CDs) were synthesized in one-pot by Michael addition copolymerization of N,N'-methylene bisacrylamide, 1-(2-aminoethyl)piperazine, and mono-6-deoxy-6-ethylenediamino-β-CD. In comparison to pure HPAA, the fluorescence intensity of HPAA-CDs was enhanced significantly while the cytotoxicity became lower. Ascribed to plenty of amino groups and strong photoluminescence, HPAA-CDs could be used as nonviral gene delivery vectors, and the corresponding gene transfection was evaluated. The experimental results indicated that HPAA-CDs condensed the plasmid DNA very well. By utilizing the fluorescent properties of HPAA-CDs, the cellular uptake and gene transfection processes were tracked by flow cytometry and confocal laser scanning microscopy without any fluorescent labeling. The transfection efficiencies of HPAA-CDs were similar to that of pure HPAA. In addition, the inner cavities of β-CDs in HPAA-CDs could be used to encapsulate drugs through host--guest interaction. Therefore, the HPAA-CDs may have potential application in the combination of gene therapy and chemotherapy.
authors.affiliation: School of Chemistry and Chemical Engineering, State Key Laboratory of Metal Matrix Composites, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.forename: Linzhu
authors.initials: L
authors.lastname: Zhou
authors.forename: Yan
authors.initials: Y
authors.lastname: Pang
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.forename: Feng
authors.initials: F
authors.lastname: Qiu
authors.forename: Xulin
authors.initials: X
authors.lastname: Jiang
authors.forename: Xinyuan
authors.initials: X
authors.lastname: Zhu
authors.forename: Deyue
authors.initials: D
authors.lastname: Yan
authors.forename: Qun
authors.initials: Q
authors.lastname: Chen
mesh_terms: D000818:Animals; D019556:COS Cells; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D004247:DNA / Q000737:chemistry / Q000235:genetics; D005434:Flow Cytometry; D005453:Fluorescence; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D049408:Luminescent Agents / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D018613:Microscopy, Confocal; D008968:Molecular Conformation; D010316:Particle Size; D010957:Plasmids; D011073:Polyamines / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D012235:Rhodamines / Q000737:chemistry / Q000494:pharmacology; D013499:Surface Properties; D014018:Tissue Distribution; D047392:beta-Cyclodextrins / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D049408:Luminescent Agents; C531249:Poly(amidoamine); D011073:Polyamines; D012235:Rhodamines; D047392:beta-Cyclodextrins; D004247:DNA; C031215:betadex; C029773:rhodamine B