title: Glycosidic bond cleavage is not required for phytosteryl glycoside-induced reduction of cholesterol absorption in mice.
abstract: Phytosteryl glycosides occur in natural foods but little is known about their metabolism and bioactivity. Purified acylated steryl glycosides (ASG) were compared with phytosteryl esters (PSE) in mice. Animals on a phytosterol-free diet received ASG or PSE by gavage in purified soybean oil along with tracers cholesterol-d(7) and sitostanol-d(4). In a three-day fecal recovery study, ASG reduced cholesterol absorption efficiency by 45 ± 6% compared with 40 ± 6% observed with PSE. Four hours after gavage, plasma and liver cholesterol-d(7) levels were reduced 86% or more when ASG was present. Liver total phytosterols were unchanged after ASG administration but were significantly increased after PSE. After ASG treatment both ASG and deacylated steryl glycosides (SG) were found in the gut mucosa and lumen. ASG was quantitatively recovered from stool samples as SG. These results demonstrate that ASG reduces cholesterol absorption in mice as efficiently as PSE while having little systemic absorption itself. Cleavage of the glycosidic linkage is not required for biological activity of ASG. Phytosteryl glycosides should be included in measurements of bioactive phytosterols.
authors.affiliation: Division of Endocrinology, Metabolism and Lipid Research, Department of Medicine, Washington University School of Medicine, Campus Box 8127, 660 South Euclid Ave., St. Louis, MO 63110, USA.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Lin
authors.forename: Lina
authors.initials: L
authors.lastname: Ma
authors.forename: Robert A
authors.initials: RA
authors.lastname: Moreau
authors.forename: Richard E
authors.initials: RE
authors.lastname: Ostlund
mesh_terms: D000818:Animals; D002784:Cholesterol / Q000378:metabolism*; D002849:Chromatography, Gas / Q000379:methods; D005243:Feces / Q000737:chemistry; D006027:Glycosides / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007408:Intestinal Absorption / Q000502:physiology*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D051379:Mice; D008810:Mice, Inbred C57BL; D010840:Phytosterols / Q000737:chemistry / Q000378:metabolism*
chemical_list: D006027:Glycosides; D010840:Phytosterols; D002784:Cholesterol