title: The genotype of the calcium/calmodulin-dependent protein kinase gene (CCaMK) determines bacterial community diversity in rice roots under paddy and upland field conditions.
abstract: The effects of the Oryza sativa calcium/calmodulin-dependent protein kinase OsCCaMK genotype (dominant homozygous [D], heterozygous [H], recessive homozygous [R]) on rice root-associated bacteria, including endophytes and epiphytes, were examined by using a Tos17 rice mutant line under paddy and upland field conditions. Roots were sampled at the flowering stage and were subjected to clone library analyses. The relative abundance of Alphaproteobacteria was noticeably decreased in R plants under both paddy and upland conditions (0.8% and 3.0%, respectively) relative to those in D plants (10.3% and 17.4%, respectively). Population shifts of the Sphingomonadales and Rhizobiales were mainly responsible for this low abundance in R plants. The abundance of Anaerolineae (Chloroflexi) and Clostridia (Firmicutes) was increased in R plants under paddy conditions. The abundance of a subpopulation of Actinobacteria (Saccharothrix spp. and unclassified Actinosynnemataceae) was increased in R plants under upland conditions. Principal coordinate analysis revealed unidirectional community shifts in relation to OsCCaMK gene dosage under both conditions. In addition, shoot length, tiller number, and plant weight decreased as the OsCCaMK gene dosage decreased under upland conditions. These results suggest significant impacts of OsCCaMK on both the diversity of root-associated bacteria and rice plant growth under both paddy and upland field conditions.
authors.affiliation: Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai 980-8577, Japan.
authors.forename: Seishi
authors.initials: S
authors.lastname: Ikeda
authors.forename: Takashi
authors.initials: T
authors.lastname: Okubo
authors.forename: Naoya
authors.initials: N
authors.lastname: Takeda
authors.forename: Mari
authors.initials: M
authors.lastname: Banba
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Sasaki
authors.forename: Haruko
authors.initials: H
authors.lastname: Imaizumi-Anraku
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Fujihara
authors.forename: Yoshinari
authors.initials: Y
authors.lastname: Ohwaki
authors.forename: Kenshiro
authors.initials: K
authors.lastname: Ohshima
authors.forename: Yoshimichi
authors.initials: Y
authors.lastname: Fukuta
authors.forename: Shima
authors.initials: S
authors.lastname: Eda
authors.forename: Hisayuki
authors.initials: H
authors.lastname: Mitsui
authors.forename: Masahira
authors.initials: M
authors.lastname: Hattori
authors.forename: Tadashi
authors.initials: T
authors.lastname: Sato
authors.forename: Takuro
authors.initials: T
authors.lastname: Shinano
authors.forename: Kiwamu
authors.initials: K
authors.lastname: Minamisawa
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000302:isolation & purification*; D044822:Biodiversity; D054737:Calcium-Calmodulin-Dependent Protein Kinase Kinase / Q000235:genetics*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005838:Genotype; D006579:Heterozygote; D006720:Homozygote; D008969:Molecular Sequence Data; D012275:Oryza / Q000201:enzymology* / Q000235:genetics / Q000382:microbiology*; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D054737:Calcium-Calmodulin-Dependent Protein Kinase Kinase