title: Evaluation of antibacterial activity of 3-butenyl, 4-pentenyl, 2-phenylethyl, and benzyl isothiocyanate in Brassica vegetables.
abstract: This study investigated antibacterial activities of 4 isothiocyanates (3-butenyl, 4-phentenyl, 2-phenylethyl, and benzyl isothiocyanate) against 4 Gram-positive bacteria (Bacillus cereus, Bacillus subtilis, Listeria monocytogenes, and Staphylococcus aureus) and 7 Gram-negative bacteria (Aeromonas hydrophila, Pseudomonas aeruginosa, Salmonella choleaesuis, Salmonella enterica, Serratia marcescens, Shigella sonnei, and Vibrio parahaemolyticus) by an agar disc diffusion assay. Benzyl isothiocyanate (> 90.00 mm inhibition zone diameter at 0.1 μL/mL) and 2-phenylethyl isothiocyanate (58.33 mm at 0.2 μL/mL) showed large inhibition zones especially against B. cereus. Also, 3-butenyl isothiocyanate (21.67 mm at 1.0 μL/mL) and 4-pentenyl isothiocyanate (19.67 mm at 1.0 μL/mL) displayed potent antibacterial activity against A. hydrophila. Benzyl and 2-phenylethyl isothiocyanate indicated higher activity against most of the pathogenic bacteria than 3-butenyl and 4-pentenyl isothiocyanate, and were more effective against Gram-positive bacteria than Gram-negative bacteria.
authors.affiliation: Plant Resources Research Inst., Duksung Women's Univ., Seoul 132-714, Korea.
authors.forename: Miran
authors.initials: M
authors.lastname: Jang
authors.forename: Eunyoung
authors.initials: E
authors.lastname: Hong
authors.forename: Gun-Hee
authors.initials: GH
authors.lastname: Kim
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001937:Brassica / Q000737:chemistry*; D052978:Disk Diffusion Antimicrobial Tests; D005520:Food Preservatives / Q000494:pharmacology*; D005961:Glucosinolates / Q000032:analysis; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D017879:Isothiocyanates / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D005520:Food Preservatives; D005961:Glucosinolates; D017879:Isothiocyanates