title: Efficient transfection method using deacylated polyethylenimine-coated magnetic nanoparticles.
abstract: Low efficiencies of nonviral gene vectors, such as transfection reagent, limit their utility in gene therapy. To overcome this disadvantage, we report on the preparation and properties of magnetic nanoparticles [diameter (d) = 121.32 ± 27.36 nm] positively charged by cationic polymer deacylated polyethylenimine (PEI max), which boosts gene delivery efficiency compare with polyethylenimine (PEI), and their use for the forced expression of plasmid delivery by application of a magnetic field. Magnetic nanoparticles were coated with PEI max, which enabled their electrostatic interaction with negatively charged molecules such as plasmid. We successfully transfected 81.1 ± 4.0% of the cells using PEI max-coated magnetic nanoparticles (PEI max-nanoparticles). Along with their superior properties as a DNA delivery vehicle, PEI max-nanoparticles offer to deliver various DNA formulations in addition to traditional methods. Furthermore, efficiency of the gene transfer was not inhibited in the presence of serum in the cells. PEI max-nanoparticles may be a promising gene carrier that has high transfection efficiency as well as low cytotoxicity.
authors.affiliation: Innovative Integration between Medicine and Engineering Based on Information Communications Technology, Yokohama National University Global COE Program, Yokohama, Japan. dkami@tmig.or.jp
authors.forename: Daisuke
authors.initials: D
authors.lastname: Kami
authors.forename: Shogo
authors.initials: S
authors.lastname: Takeda
authors.forename: Hatsune
authors.initials: H
authors.lastname: Makino
authors.forename: Masashi
authors.initials: M
authors.lastname: Toyoda
authors.forename: Yoko
authors.initials: Y
authors.lastname: Itakura
authors.forename: Satoshi
authors.initials: S
authors.lastname: Gojo
authors.forename: Shunei
authors.initials: S
authors.lastname: Kyo
authors.forename: Akihiro
authors.initials: A
authors.lastname: Umezawa
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Watanabe
mesh_terms: D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D004337:Drug Carriers; D051379:Mice; D053758:Nanoparticles; D011094:Polyethyleneimine; D014162:Transfection / Q000379:methods*
chemical_list: D004337:Drug Carriers; D011094:Polyethyleneimine