title: Overexpression of activin A inhibits growth, induces apoptosis, and suppresses tumorigenicity in an androgen-sensitive human prostate cancer cell line LNCaP.
abstract: The effects of overexpression of activin A in the androgen-sensitive human prostate cancer cell line LNCaP were studied. A full-length cDNA of activin beta A coding region was inserted into a eukaryotic expression vector and transfected into the LNCaP cells. Overexpression of activin BA significantly inhibited growth of this cell line. An increased death rate was also noted in these activin-overproducing cells, which was believed to be due to apoptosis as manifested by morphological change, DNA laddering, and FAGS analysis. The expression of bcl-2 was suppressed and the expression of c-myc was stimulated in these cells. In addition, the efficiency of soft agar colony formation and the tumorigenicity in the nude mice were suppressed for the activin producing LNCaP cells.
authors.affiliation: UNIV SO CALIF,DEPT CELL & NEUROBIOL,SCH MED,LOS ANGELES,CA 90033. UNIV SO CALIF,DEPT PATHOL,SCH MED,LOS ANGELES,CA 90033.
authors.forename: Z
authors.initials: Z
authors.lastname: Zhang
authors.forename: J
authors.initials: J
authors.lastname: Zheng
authors.forename: Y
authors.initials: Y
authors.lastname: Zhao
authors.forename: G
authors.initials: G
authors.lastname: Li
authors.forename: Y
authors.initials: Y
authors.lastname: Batres
authors.forename: M
authors.initials: M
authors.lastname: Luo
authors.forename: M
authors.initials: M
authors.lastname: Wan
authors.forename: S
authors.initials: S
authors.lastname: Ying