title: [Cloning and sequence analysis of a new cathepsin L-like cysteine proteinase gene from Ditylenchus destructor].
abstract: The Cathepsin L-like cysteine proteinase genes (cpls) are multifunction genes related to the parasitic abilities of plant parasitic nematodes. A new cathepsin L-like cysteine proteinase gene (Dd-cpl-1) (GenBank Accession GQ 180107) was cloned from Ditylenchus destructor by RT-PCR and RACE. The cDNA sequence consisted of a 1 131 bp open reading frame (ORF) encoding 376 amino acid residues that were franked by a 29 bp 5'-untranslated region (UTR) and a 159 bp 3'-UTR. Genomic sequence analysis showed that Dd-cpl-1 contained 7 introns, obeyed the GT/AG rule in the splice-site junctions. Homology analysis showed that the identity was 77% between Dd-cpl-1 deduced protein Dd-CPL-1 and cathepsin L-like cysteine proteinase of Bursaphelenchus xylophilus. Multi-sequence alignment indicated that there were the catalytic triad (Cys183, His322 and Asn343) and two motifs ERFNIN motif and GNFD motif in deduced protein Dd-CPL-1. Cysteine proteinases phylogenetic analysis showed that Dd-cpl-1 belonged to the sub-clade of cathepsin L-like cysteine proteinases.
authors.affiliation: College of Life Sciences, Tianjin Normal University, Tianjin 300387, China.
authors.forename: Gaofeng
authors.initials: G
authors.lastname: Wang
authors.forename: Deliang
authors.initials: D
authors.lastname: Peng
authors.forename: Jianhua
authors.initials: J
authors.lastname: Sun
authors.forename: Wenkun
authors.initials: W
authors.lastname: Huang
authors.forename: Huan
authors.initials: H
authors.lastname: Peng
authors.forename: Haibo
authors.initials: H
authors.lastname: Long
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D056668:Cathepsin L / Q000235:genetics*; D003001:Cloning, Molecular; D057056:Cysteine Proteases / Q000235:genetics*; D017238:Genes, Helminth / Q000235:genetics*; D008969:Molecular Sequence Data; D009348:Nematoda / Q000201:enzymology* / Q000235:genetics; D010802:Phylogeny; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D011198:Solanum tuberosum / Q000469:parasitology*
chemical_list: D057056:Cysteine Proteases; D056668:Cathepsin L