title: Paxillin and phospholipase D interact to regulate actin-based processes in Dictyostelium discoideum.
abstract: The actin cytoskeleton forms a membrane-associated network whose proper regulation is essential for numerous processes, including cell differentiation, proliferation, adhesion, chemotaxis, endocytosis, exocytosis, and multicellular development. In this report, we show that in Dictyostelium discoideum, paxillin (PaxB) and phospholipase D (PldB) colocalize and coimmunoprecipitate, suggesting that they interact physically. Additionally, the phenotypes observed during development, cell sorting, and several actin-required processes, including cyclic AMP (cAMP) chemotaxis, cell-substrate adhesion, actin polymerization, phagocytosis, and exocytosis, reveal a genetic interaction between paxB and pldB, suggesting a functional interaction between their gene products. Taken together, our data point to PldB being a required binding partner of PaxB during processes involving actin reorganization.
authors.affiliation: Department of Biological Sciences, Hunter College, 695 Park Avenue, New York, NY 10065, USA.
authors.forename: Jelena
authors.initials: J
authors.lastname: Pribic
authors.forename: Rebecca
authors.initials: R
authors.lastname: Garcia
authors.forename: May
authors.initials: M
authors.lastname: Kong
authors.forename: Derrick
authors.initials: D
authors.lastname: Brazill
mesh_terms: D000199:Actins / Q000378:metabolism; D002448:Cell Adhesion; D002454:Cell Differentiation; D002465:Cell Movement; D049109:Cell Proliferation; D000242:Cyclic AMP / Q000378:metabolism; D003599:Cytoskeleton / Q000378:metabolism; D004023:Dictyostelium / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D004705:Endocytosis / Q000502:physiology; D005089:Exocytosis / Q000502:physiology; D055786:Gene Knockout Techniques; D047468:Immunoprecipitation; D051419:Paxillin / Q000235:genetics* / Q000378:metabolism; D010587:Phagocytosis / Q000502:physiology; D010739:Phospholipase D / Q000235:genetics* / Q000378:metabolism; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D000199:Actins; D051419:Paxillin; D015800:Protozoan Proteins; D000242:Cyclic AMP; D010739:Phospholipase D