title: Expression of β defensins 1, 3 and 4 in chorioamniotic membranes of preterm pregnancies complicated by chorioamnionitis.
abstract: OBJECTIVE
  To quantify the expression of human β-defensins (HBDs) 1, 3 and 4 in chorioamniotic membranes in pregnancies complicated by prematurity associated with histologic chorioamnionitis.
  
  
  METHODS
  The study group included 23 fragments of chorioamniotic membranes with histologic chorioamnionitis from women with preterm premature rupture of membranes (PPROM) and preterm labor (PTL) with intact membranes, who had preterm deliveries. As a control group, 30 chorioamniotic membranes without chorioamnionitis at the same gestational age as those in the study group were included. Chorioamniotic membranes were collected for histopathological analyses, and HBD mRNA expression quantification was analyzed by real time PCR. Comparisons were performed using the Mann-Whitney or Kruskal-Wallis tests and all the women were informed and provided written consent.
  
  
  RESULTS
  The expression of HBDs mRNA in the fetal membranes was similar in patients without histologic chorioamnionitis (HBD1: 0.7-fold, HBD3: 0.9-fold, HBD4: 0.3-fold) compared to patients with chorioamnionitis (HBD1: 1.1-fold, HBD3: 0.9-fold, HBD4: 0.4-fold; p>0.05). Regarding the gestational complications that resulted in premature delivery, PPROM or PTL, the relative quantification of HBD1, HBD3 and HBD4 showed no statistically significant differences in either the absence or presence of chorioamnionitis. Among patients with histologic chorioamnionitis, patients with PPROM (HBD1: 2.7-fold, HBD3: 0.3-fold, HBD4: 0.7-fold) presented similar mRNA expression to those with PTL (HBD1: 0.7-fold, HBD3: 1.2-fold, HBD4: 0.13-fold; p>0.05).
  
  
  CONCLUSIONS
  Chorioamniotic membranes are sources of β defensins 1, 3 and 4; however, considering the sample size and the methodology applied, mRNA concentrations were not related to the presence of histologic chorioamnionitis.
authors.affiliation: Faculdade de Medicina de Botucatu, UNESP-Univ Estadual Paulista, Departmento de Patologia, São Paulo, Brazil.
authors.forename: Jossimara
authors.initials: J
authors.lastname: Polettini
authors.forename: Juliana
authors.initials: J
authors.lastname: Takitane
authors.forename: José C
authors.initials: JC
authors.lastname: Peraçoli
authors.forename: Márcia G
authors.initials: MG
authors.lastname: Silva
mesh_terms: D000293:Adolescent; D000650:Amnion / Q000378:metabolism*; D002821:Chorioamnionitis / Q000378:metabolism*; D002823:Chorion / Q000378:metabolism*; D005260:Female; D005322:Premature Rupture of Fetal Membranes / Q000378:metabolism; D005865:Gestational Age; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature / Q000378:metabolism*; D007752:Obstetric Labor, Premature / Q000378:metabolism; D011247:Pregnancy; D011248:Pregnancy Complications / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D055815:Young Adult; D023083:beta-Defensins / Q000378:metabolism*
chemical_list: C406407:DEFB1 protein, human; C422950:DEFB103A protein, human; C106641:DEFB4A protein, human; D012333:RNA, Messenger; D023083:beta-Defensins