title: MicroRNA-143 functions as a tumor suppressor in human bladder cancer T24 cells.
abstract: MicroRNA (miR)-143 and -145 were down-regulated in human bladder cancer T24 cells. The enforced expression of miR-143 induced growth-suppression in T24 cells through down-regulation of ERK5 and Akt expression at translational level, and chemically-modified synthetic miR-143 (miR-143/BP) exhibited a greater growth inhibitory effect than wild-type miR-143. In addition, the synthetic miR-143/BP induced apoptotic cell death in some of the transfected cells. Furthermore, co-treatment with the synthetic miR-143/BP and cisplatin showed the additive growth-suppressing effect on T24 cells. These findings suggest that the chemically-modified synthetic miR-143 functions as a tumor suppressor in T24 cells by targeting ERK5 and/or Akt.
authors.affiliation: The United Graduate School of Veterinary Sciences, Gifu University, Yanagido, Gifu, Japan. o5104401@edu.gifu-u.ac.jp
authors.forename: Syunsuke
authors.initials: S
authors.lastname: Noguchi
authors.forename: Takashi
authors.initials: T
authors.lastname: Mori
authors.forename: Yuki
authors.initials: Y
authors.lastname: Hoshino
authors.forename: Kohji
authors.initials: K
authors.lastname: Maruo
authors.forename: Nami
authors.initials: N
authors.lastname: Yamada
authors.forename: Yukio
authors.initials: Y
authors.lastname: Kitade
authors.forename: Tomoki
authors.initials: T
authors.lastname: Naoe
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Akao
mesh_terms: D001483:Base Sequence; D015153:Blotting, Western; D045744:Cell Line, Tumor; D017931:DNA Primers; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D001749:Urinary Bladder Neoplasms / Q000473:pathology*
chemical_list: D017931:DNA Primers; C542359:MIRN143 microRNA, human; D035683:MicroRNAs