title: Preparation of reactive oxygen scavenging peptides from tilapia (Oreochromis niloticus) skin gelatin: optimization using response surface methodology.
abstract: Gelatin extracted from tilapia skin was hydrolyzed with Properase E. Response surface methodology (RSM) was applied to optimize the hydrolysis condition (temperature [T], enzyme-to-substrate ratio [E/S], pH and reaction time [t]), to obtain the hydrolysate with the highest hydroxyl radical (•OH) scavenging activity. The optimum conditions obtained were T of 44.2 °C, E/S of 2.2%, pH of 9.2, and t of 3.4 h. The predicted •OH scavenging activity of the hydrolysate under the optimum conditions was 60.7%, and the actually experimental scavenging activity was 60.8%. The hydrolysate was fractionated by ultrafiltration, and 4 fractions were collected. The fraction TSGH4 (MW<2000 Da) showed the strongest •OH scavenging activity with the highest yield. Furthermore, reactive oxygen species (ROS) scavenging activities of TSGH4 with different concentrations were investigated in 5 model systems, including superoxide anion radical (•O2), •OH, hydrogen peroxide (H2O2), peroxynitrite (ONOO-), and nitric oxide (NO•), compared with reduced glutathione (GSH). The results showed that TSGH4 significantly scavenged these ROS, and could be used as a functional ingredient in medicine and food industries.
authors.affiliation: Research Center of Food Engineering, College of Chemistry and Engineering, Kunming University of Science and Technology, Kunming, Yunnan 650224, China. kmylzhuang@yahoo.com.cn
authors.forename: Yongliang
authors.initials: Y
authors.lastname: Zhuang
authors.forename: Liping
authors.initials: L
authors.lastname: Sun
mesh_terms: D000818:Animals; D023681:Cichlids; D029941:Fish Proteins / Q000737:chemistry / Q000191:economics / Q000302:isolation & purification* / Q000378:metabolism; D005503:Food Additives / Q000737:chemistry / Q000191:economics / Q000302:isolation & purification / Q000378:metabolism; D005525:Food-Processing Industry / Q000191:economics; D016166:Free Radical Scavengers / Q000737:chemistry / Q000191:economics / Q000302:isolation & purification* / Q000378:metabolism; D005780:Gelatin / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007220:Industrial Waste / Q000032:analysis / Q000191:economics; D007700:Kinetics; D008970:Molecular Weight; D010447:Peptide Hydrolases / Q000378:metabolism; D010455:Peptides / Q000737:chemistry / Q000191:economics / Q000302:isolation & purification* / Q000378:metabolism; D011492:Protein Hydrolysates / Q000737:chemistry; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors*; D012867:Skin / Q000737:chemistry*; D013223:Statistics as Topic; D013696:Temperature; D014462:Ultrafiltration
chemical_list: D029941:Fish Proteins; D005503:Food Additives; D016166:Free Radical Scavengers; D007220:Industrial Waste; D010455:Peptides; D011492:Protein Hydrolysates; D017382:Reactive Oxygen Species; D005780:Gelatin; D010447:Peptide Hydrolases