title: Orange-spotted grouper (Epinephelus coioides) TLR2, MyD88 and IL-1β involved in anti-Cryptocaryon irritans response.
abstract: Cryptocaryon irritans is one of the most important ectoparasites of marine fish, and can have a devastating effect on aquacultured fish populations. The role of TLR signaling pathways in anti-parasitic immune responses is poorly understood in fish. In this paper, we first cloned Epinephelus coioides MyD88 full-length cDNA (EcMyD88) and its respective gene. The open reading frame (ORF) of cDNA is 873bp encoding 291 amino acid residues. Similar to other species, the EcMyD88 gene contains of five conserved exons and four diverse introns. The constitutive expression of EcMyD88 was detected in the gill, trunk kidney, head kidney, spleen, and heart in high concentrations, while the skin, brain, liver, and muscles contained much lower titers, indicating that EcMyD88 may play a crucial role in host innate immunologic surveillance. To identify the potential role of TLR pathways in fish anti-C. irritans immune responses, we chose three important molecules involved in anti-parasite responses, TLR2, MyD88 and IL-1β to indicate TLR pathway's signal-in, signal transduction, and signal-out functions, respectively. The expression profile of these three genes was detected in grouper infected by C. irritans. Results showed these molecules each experience significant changes within the skin, gill (two infected mucosal sites), head kidney and spleen (two systematic immune organs) after C. irritans infection. These findings indicate the TLR signaling pathway may play an important role in host defense against C. irritans.
authors.affiliation: Key Laboratory for Aquatic Products Safety of Ministry of Education/State Key Laboratory of Biocontrol, The School of Life Sciences, Sun Yat-sen University, 135 Xingang West Street, Haizhu District, Guangzhou 510275, Guangdong Province, PR China.
authors.forename: Yan-Wei
authors.initials: YW
authors.lastname: Li
authors.forename: Xiao-Chun
authors.initials: XC
authors.lastname: Luo
authors.forename: Xue-Ming
authors.initials: XM
authors.lastname: Dan
authors.forename: Xia-Zi
authors.initials: XZ
authors.lastname: Huang
authors.forename: Wei
authors.initials: W
authors.lastname: Qiao
authors.forename: Zheng-Ping
authors.initials: ZP
authors.lastname: Zhong
authors.forename: An-Xing
authors.initials: AX
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016770:Ciliophora Infections / Q000276:immunology / Q000662:veterinary*; D003001:Cloning, Molecular; D016000:Cluster Analysis; D019295:Computational Biology; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D005393:Fish Diseases / Q000276:immunology* / Q000469:parasitology*; D040461:Gene Components; D053583:Interleukin-1beta / Q000276:immunology* / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D053594:Myeloid Differentiation Factor 88 / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D010473:Perciformes; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D015398:Signal Transduction / Q000276:immunology*; D051195:Toll-Like Receptor 2 / Q000276:immunology* / Q000378:metabolism
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D053583:Interleukin-1beta; D053594:Myeloid Differentiation Factor 88; D051195:Toll-Like Receptor 2