title: Chlamydia trachomatis ompA genotypes in male patients with urethritis in Greece: conservation of the serovar distribution and evidence for mixed infections with Chlamydophila abortus.
abstract: PCR amplification and nucleotide sequencing of the ompA gene of Chlamydia trachomatis were used to determine the prevalence and distribution of genotypes in 51 urine and urethral specimens from Greek male patients with urethritis, that were positive by the COBAS Amplicor test. A single C. trachomatis serovar was identified in 43 of the 51 amplified samples. Serovars F and E were the most prevalent (both 12, 28%), followed by D (9, 21%), G (4, 9%), B and K (both 2, 5%) and H and J (both 1, 2%). Over one third of the samples bared a variant ompA genotype that had been previously identified in other areas worldwide. Two results in this study, both observed for the first time, were of particular interest. First, the emergence of the unique variant genotype D/Ep6 (X77364.2) identified in 3 urethral samples. Second, the ompA genotype OCLH196 of the animal pathogen Chlamydophila abortus as well as a 23S rRNA gene fragment of this species detected by the assay ArrayTube™ was found in 7 urethral samples. The implications resulting from this observation for the health of the general population are discussed.
authors.affiliation: Laboratory of Biotechnology, Hellenic Pasteur Institute, Athens, Greece.
authors.forename: Panagiotis
authors.initials: P
authors.lastname: Psarrakos
authors.forename: Eleni
authors.initials: E
authors.lastname: Papadogeorgakis
authors.forename: Konrad
authors.initials: K
authors.lastname: Sachse
authors.forename: Evangelia
authors.initials: E
authors.lastname: Vretou
mesh_terms: D000328:Adult; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D015373:Bacterial Typing Techniques / Q000379:methods*; D002690:Chlamydia Infections / Q000453:epidemiology / Q000469:parasitology*; D002692:Chlamydia trachomatis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D023501:Chlamydophila / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005838:Genotype; D006115:Greece; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D012338:RNA, Ribosomal, 23S; D014526:Urethritis / Q000469:parasitology*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D012338:RNA, Ribosomal, 23S; C078167:OMPA outer membrane proteins