title: Immunization with dendritic cells transfected in vivo with HIV-1 plasmid DNA induces HIV-1-specific immune responses.
abstract: We evaluated the importance of dendritic cells (DCs) in the induction of the immune response after immunization of mice with DNA plasmid Auxo-GTU(®)-MultiHIV. First, GTU(®)-encoded protein was shown to be expressed by DCs of the draining lymph nodes (LNs) following intradermal (i.d.) immunization. Next, donor mice were immunized with the MultiHIV DNA plasmid, and DCs were enriched and further used to immunize naïve recipient mice. For the first time, the results show that i.d. immunization with Auxo-GTU(®)-MultiHIV transfects DCs in vivo, enabling them to present antigens and induce HIV-specific immune responses in recipient mice.
authors.affiliation: FIT Biotech Oy, Biokatu 8, 33520 Tampere, Finland. maria.malm@fitbiotech.com
authors.forename: Maria
authors.initials: M
authors.lastname: Malm
authors.forename: Kai
authors.initials: K
authors.lastname: Krohn
authors.forename: Vesna
authors.initials: V
authors.lastname: Blazevic
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology; D000956:Antigens, Viral; D004279:DNA, Viral / Q000235:genetics / Q000276:immunology*; D003713:Dendritic Cells; D015658:HIV Infections / Q000276:immunology / Q000517:prevention & control*; D015497:HIV-1 / Q000276:immunology*; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids / Q000235:genetics*; D013154:Spleen / Q000166:cytology
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D004279:DNA, Viral; D007074:Immunoglobulin G