title: Vacuum sealing and cooling as methods to preserve surgical specimens.
abstract: Recently, vacuum-based preservation of surgical specimens has been proposed as a safe alternative to formalin fixation at the surgical theater. The method seems feasible from a practical point of view, but no systematic study has examined the effect of vacuum sealing alone with respect to tissue preservation. In this study, we therefore subjected tissue samples from 5 different organs to treatments with and without vacuum sealing and cooling at 4°C to study the effect of vacuum sealing of surgical specimens with respect to tissue preservation and compare it with the effect of cooling. No preserving effect of vacuum sealing was observed with respect to cellular morphology, detection of immunohistochemical epitopes, or RNA integrity. In contrast, storage at 4°C was shown to preserve tissue to a higher degree than storage at room temperature for all included endpoints, independently of whether the tissue was subjected to vacuum sealing or not. We, therefore, conclude that vacuum sealing is not an alternative to cooling on ice.
authors.affiliation: Department of Pathology, Odense University Hospital, Denmark. thomas.kristensen@ouh.regionsyddanmark.dk
authors.forename: Thomas
authors.initials: T
authors.lastname: Kristensen
authors.forename: Birte
authors.initials: B
authors.lastname: Engvad
authors.forename: Ole
authors.initials: O
authors.lastname: Nielsen
authors.forename: Torsten
authors.initials: T
authors.lastname: Pless
authors.forename: Steen
authors.initials: S
authors.lastname: Walter
authors.forename: Martin
authors.initials: M
authors.lastname: Bak
mesh_terms: D003080:Cold Temperature; D006801:Humans; D007150:Immunohistochemistry; D014021:Tissue Preservation / Q000379:methods* / Q000592:standards; D014618:Vacuum