title: Fast copper-free click DNA ligation by the ring-strain promoted alkyne-azide cycloaddition reaction.
abstract: Templated DNA strand ligation by the ring-strain promoted alkyne-azide [3+2] cycloaddition reaction is very fast; with dibenzocyclooctyne, the reaction is essentially complete in 1 min. It is inhibited by the presence of a single mismatched base pair suggesting applications in genetic analysis.
authors.affiliation: School of Chemistry, University of Southampton, Highfield, Southampton, SO17 1BJ, UK.
authors.forename: Montserrat
authors.initials: M
authors.lastname: Shelbourne
authors.forename: Xiong
authors.initials: X
authors.lastname: Chen
authors.forename: Tom
authors.initials: T
authors.lastname: Brown
authors.forename: Afaf H
authors.initials: AH
authors.lastname: El-Sagheer
mesh_terms: D000480:Alkynes / Q000737:chemistry*; D001386:Azides / Q000737:chemistry*; D057930:Click Chemistry; D003300:Copper / Q000737:chemistry; D003500:Cyclization; D004247:DNA / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D009841:Oligonucleotides / Q000737:chemistry
chemical_list: D000480:Alkynes; D001386:Azides; D005456:Fluorescent Dyes; D009841:Oligonucleotides; D003300:Copper; D004247:DNA