title: Dual function of a tip fimbrillin of Actinomyces in fimbrial assembly and receptor binding.
abstract: Interaction of Actinomyces oris with salivary proline-rich proteins (PRPs), which serve as fimbrial receptors, involves type 1 fimbriae. Encoded by the gene locus fimQ-fimP-srtC1, the type 1 fimbria is comprised of the fimbrial shaft FimP and the tip fimbrillin FimQ. Fimbrial polymerization requires the fimbria-specific sortase SrtC1, which catalyzes covalent linkage of fimbrial subunits. Using genetics, biochemical methods, and electron microscopy, we provide evidence that the tip fimbrillin, FimQ, is involved in fimbrial assembly and interaction with PRPs. Specifically, while deletion of fimP completely abolished the type 1 fimbrial structures, surface display of monomeric FimQ was not affected by this mutation. Surprisingly, deletion of fimQ significantly reduced surface assembly of the type 1 fimbriae. This defect was rescued by recombinant FimQ ectopically expressed from a plasmid. In agreement with the role of type 1 fimbriae in binding to PRPs, aggregation of A. oris with PRP-coated beads was abrogated in cells lacking srtC1 or fimP. This aggregation defect of the ΔfimP mutant was mainly due to significant reduction of FimQ on the bacterial surface, as the aggregation was not observed in a strain lacking fimQ. Increasing expression of FimQ in the ΔfimP mutant enhanced aggregation, while overexpression of FimP in the ΔfimQ mutant did not. Furthermore, recombinant FimQ, not FimP, bound surface-associated PRPs in a dose-dependent manner. Thus, not only does FimQ function as the major adhesin of the type 1 fimbriae, it also plays an important role in fimbrial assembly.
authors.affiliation: Department of Microbiology and Molecular Genetics, University of Texas Health Science Center, Houston, TX 77030, USA.
authors.forename: Chenggang
authors.initials: C
authors.lastname: Wu
authors.forename: Arunima
authors.initials: A
authors.lastname: Mishra
authors.forename: Jinghua
authors.initials: J
authors.lastname: Yang
authors.forename: John O
authors.initials: JO
authors.lastname: Cisar
authors.forename: Asis
authors.initials: A
authors.lastname: Das
authors.forename: Hung
authors.initials: H
authors.lastname: Ton-That
mesh_terms: D000190:Actinomyces / Q000378:metabolism*; D001422:Bacterial Adhesion; D036781:Fimbriae Proteins / Q000235:genetics / Q000378:metabolism*; D010861:Fimbriae, Bacterial / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D017353:Gene Deletion; D005816:Genetic Complementation Test; D046911:Macromolecular Substances / Q000378:metabolism / Q000648:ultrastructure; D011485:Protein Binding; D055503:Protein Multimerization
chemical_list: D046911:Macromolecular Substances; C063055:fimbrillin; D036781:Fimbriae Proteins