title: Evaluation of the URIT-2900 automated hematology analyzer for screening of thalassemia and hemoglobinopathies in Southeast Asian populations.
abstract: BACKGROUND
  The effectiveness of the URIT-2900 Hematology Analyzer for screening of hemoglobinopathies commonly found in Southeast Asian populations was examined.
  
  
  METHODS
  Appropriate cut-off values of MCV and MCH for screening of α(0) and β thalassemias were derived from the receiver operator characteristic curve conducted initially on 279 subjects with various thalassemia genotypes. Validation was performed additionally in a cohort of another unrelated 313 subjects.
  
  
  RESULTS
  The best cut off values of MCV and MCH were found to be 78fL and 27pg, respectively. Using these cut off values in combination with the dichlorophenolindophenol test in screening of α(0) thalassemia, β thalassemia and Hb E in a cohort study revealed 100% sensitivity, 79.6% specificity, 80.0% positive predictive value and 100% negative predictive value.
  
  
  CONCLUSIONS
  The combined blood cell counting using the URIT-2900 Automated Hematology Analyzer and dichlorophenolindophenol test is suitable for population screening of thalassemia and hemoglobinopathies in Southeast Asia.
authors.affiliation: The Graduate School, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.
authors.forename: Rossarin
authors.initials: R
authors.lastname: Karnpean
authors.forename: Anupong
authors.initials: A
authors.lastname: Pansuwan
authors.forename: Goonnapa
authors.initials: G
authors.lastname: Fucharoen
authors.forename: Supan
authors.initials: S
authors.lastname: Fucharoen
mesh_terms: D001210:Asia, Southeastern; D001331:Automation; D016922:Cellular Senescence; D015331:Cohort Studies; D004909:Erythrocyte Indices; D005838:Genotype; D006405:Hematology / Q000295:instrumentation*; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D008403:Mass Screening; D012372:ROC Curve; D015203:Reproducibility of Results; D013789:Thalassemia / Q000097:blood / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology
chemical_list: D006454:Hemoglobins