title: Preparation of decellularized and crosslinked artery patch for vascular tissue-engineering application.
abstract: There is an urgent clinical need of tissue-engineering (TE) vascular grafts, so this study was for developing a fast and simple way of producing TE vascular scaffold. The TE vascular scaffold was prepared with pepsin, DNase and RNase enzymatic decellularization and crosslinked with 0.1, 1, 5% glutaraldehyde (GA), respectively. The samples were underwent analyses of burst pressure; suture strength; cytotoxicity; enzymatic degradation in vitro; degradation in vivo; rehydration; biocompatibilities detected with hematoxylin and eosin (H&E), scan electron microscope, immunohistochemistry both in vivo and in vitro; macrophage infiltration and calcification using Von Kossa staining. After being decellularized the scaffold had a complete removal of cellular components, an intact collagen structure. The burst pressure and suture strength were similar to native artery. 0.1% GA crosslinked scaffold showed less cytotoxicity than 1 and 5% GA groups (P < 0.05) and was resistance to enzymatic degradation in vitro. Once being implanted, 0.1% GA group was resistant to degradation and formed endothelium, smooth muscle and adventitia with few macrophages infiltration. However, there appeared calcification in implants compared with that in native artery. This study demonstrated that DVPs producing methods by enzymatic decellularizing and crosslinking with 0.1% GA could be used for clinical TE vascular graft manufacture.
authors.affiliation: Department of Vascular Surgery, Zhongshan Hospital, Xiamen University, Xiamen, China.
authors.forename: Yilin
authors.initials: Y
authors.lastname: Zhao
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Zhang
authors.forename: Jinling
authors.initials: J
authors.lastname: Wang
authors.forename: Ping
authors.initials: P
authors.lastname: Yin
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Yin
authors.forename: Jianyin
authors.initials: J
authors.lastname: Zhou
authors.forename: Gang
authors.initials: G
authors.lastname: Xu
authors.forename: Yun
authors.initials: Y
authors.lastname: Liu
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Deng
authors.forename: Maochuan
authors.initials: M
authors.lastname: Zhen
authors.forename: Wugeng
authors.initials: W
authors.lastname: Cui
authors.forename: Zhongchen
authors.initials: Z
authors.lastname: Liu
mesh_terms: D000818:Animals; D001158:Arteries / Q000166:cytology* / Q000187:drug effects; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000494:pharmacology; D001807:Blood Vessel Prosthesis; D002454:Cell Differentiation; D002478:Cells, Cultured; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004798:Enzymes / Q000378:metabolism; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D010012:Osteogenesis / Q000187:drug effects / Q000502:physiology; D012756:Sheep; D023822:Tissue Engineering / Q000379:methods*; D058017:Vascular Grafting / Q000295:instrumentation / Q000379:methods
chemical_list: D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D004798:Enzymes