title: In vitro anticancer activity of doxorubicin-loaded gelatin-coated magnetic iron oxide nanoparticles.
abstract: Magnetic drug targeting allows accumulation of drug at a defined target site with the help of an external magnetic field. Current research explored uptake and anticancer activity of doxorubicin-loaded gelatin-coated magnetic iron oxide particles (DXR-GIOPs) in order to investigate potential of gelatin-coated iron oxide particles (GIOPs) as a drug carrier in the field of magnetic drug targeting. The in vitro test was done using HeLa cells as a model cell and DXR as a model drug. The cytotoxicity and uptake of GIOPs were also studied and results were compared with that of DXR-GIOPs. The results indicated that GIOPs were not toxic to HeLa cells even at higher concentration of 1.2 mg/mL; however, DXR-GIOPs showed toxicity in time as well as dose-dependent manner. Furthermore, quantitative and qualitative uptake studies showed higher uptake of DXR-GIOPs compared to GIOPs in the identical condition by the cells.
authors.affiliation: School of Mechanical, Material and Mechatronic Engineering, University of Wollongong, Wollongong, NSW, Australia.
authors.forename: Babita
authors.initials: B
authors.lastname: Gaihre
authors.forename: Myung S
authors.initials: MS
authors.lastname: Khil
authors.forename: Hak Y
authors.initials: HY
authors.lastname: Kim
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D004337:Drug Carriers / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor / Q000379:methods; D005290:Ferric Compounds / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D005780:Gelatin / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D053758:Nanoparticles
chemical_list: D000903:Antibiotics, Antineoplastic; D004337:Drug Carriers; D005290:Ferric Compounds; C000499:ferric oxide; D004317:Doxorubicin; D005780:Gelatin