title: Morphologic and mitochondrial characterization of human spermatogenic cells dispersed in wet preparation for testicular sperm extraction: establishment of a microscopic diagram of developing human spermatogenic cells.
abstract: The successful identification of spermatozoa at microdissection testicular sperm extraction is critical and can depend on the judgment of experienced reproductive clinicians. Therefore, we characterized human spermatogenic cells based on stage-specific mitochondrial location and morphologic change by using a vital mitochondrion-specific fluorescent probe, Mitotracker, and stage-specific antibodies to make a useful microscopic diagram.
authors.affiliation: Department of Urology, Osaka University Graduate School of Medicine, Osaka, Japan.
authors.forename: Hidenobu
authors.initials: H
authors.lastname: Okuda
authors.forename: Akira
authors.initials: A
authors.lastname: Tsujimura
authors.forename: Keisuke
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Fukuhara
authors.forename: Jiro
authors.initials: J
authors.lastname: Nakayama
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kiuchi
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Takao
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Miyagawa
authors.forename: Norio
authors.initials: N
authors.lastname: Nonomura
authors.forename: Akihiko
authors.initials: A
authors.lastname: Okuyama
mesh_terms: D048430:Cell Shape; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D006801:Humans; D008297:Male; D042282:Microdissection; D008856:Microscopy, Fluorescence / Q000379:methods; D008928:Mitochondria / Q000648:ultrastructure*; D009930:Organic Chemicals; D053625:Sperm Retrieval; D013091:Spermatogenesis; D013094:Spermatozoa / Q000648:ultrastructure*; D013737:Testis / Q000166:cytology / Q000648:ultrastructure*
chemical_list: D005456:Fluorescent Dyes; D009930:Organic Chemicals; C107472:red dye CMXRos