title: Temperature-response polymer coating for in-tube solid-phase microextraction coupled to high-performance liquid chromatography.
abstract: The silica nanoparticle (SiO(2) NP)-deposited capillary fabricated by liquid phase deposition (LPD) was bonded by 3-(triethoxysilyl) propyl methacrylate and then modified with poly(N-isopropylacrylamide) (PNIPAAm) by polymerization. The resulting PNIPAAm modified SiO(2) NP-deposited capillary was applied to in-tube solid-phase microextraction coupled to high-performance liquid chromatography (in-tube SPME-HPLC). To investigate the extraction performance of the prepared capillary, diethylstilbestrol (DES) with moderate polarity was selected as the model analyte. Results demonstrate that PNIPAAm modified SiO(2) NP-deposited capillary exhibited obvious temperature responsive character. Finally, the PNIPAAm modified SiO(2) NP-deposited capillary was applied to the analysis of three synthetical estrogens from milk samples. The detection limit of the method was found to be in the range 1.2-2.2 ng/g, and recovery was 71.7-98.9% with relative standard deviations in the range of 2.8-12.6%.
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072, China.
authors.forename: Qiong-Wei
authors.initials: QW
authors.lastname: Yu
authors.forename: Qiao
authors.initials: Q
authors.lastname: Ma
authors.forename: Yu-Qi
authors.initials: YQ
authors.lastname: Feng
mesh_terms: D000178:Acrylamides / Q000737:chemistry*; D000180:Acrylic Resins; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004967:Estrogens / Q000032:analysis / Q000302:isolation & purification; D008892:Milk / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry; D052617:Solid Phase Microextraction / Q000379:methods*; D013696:Temperature; D013997:Time Factors
chemical_list: D000178:Acrylamides; D000180:Acrylic Resins; D004967:Estrogens; D011108:Polymers; C052970:poly-N-isopropylacrylamide; D012822:Silicon Dioxide