title: Ectopic expression of Col2.3 and Col3.6 promoters in the brain and association with leptin signaling.
abstract: The collagen 2.3 and 3.6 promoters have been used to drive Cre expression for generation of conditional transgenic mutant mice. Within the bone, Col3.6 is expressed by mesenchymal precursor cells and their downstream progeny, while Col2.3 is more osteoblast specific. Our generation of transgenic mice with Col2.3-Cre- and Col3.6-Cre-driven deletion of the long-form leptin receptor (ObRb) necessitated a thorough analysis of the nonspecific expression of these promoters in the central nervous system. Both Col2.3 and Col3.6 were capable of forcing loxP recombination in the brain as demonstrated by EGFP expression in ROSA reporter mice. Expression of Col2.3 was limited to the central base of the brain near the third ventricle. In contrast, robust expression of Col3.6 was noted throughout the brain, centering near the distal third ventricle, third ventricle, and aqueduct. We subsequently analyzed the colocalization of leptin-responsive P-Stat3 neurons with Col3.6-expressing neurons. Approximately 5-10% colocalization was noted in leptin-responsive brain areas such as the arcuate nucleus, dorsal medial hypothalamus, ventral premammillary nucleus, and lateral hypothalamus. Injection of 3.6(Cre+F/F) ObRb knockout mice with leptin confirmed the presence of an intact P-Stat3 response that was dampened in the lateral hypothalamus (p < 0.050). This test was done to explore the contribution of neural leptin signaling to the bone phenotype of the 3.6(Cre+F/F) mice. Our analysis indicates that neural ObRb deletion, while present, is likely not the sole driver of femoral changes through traditional sympathetic circuits.
authors.affiliation: University of Michigan School of Dentistry, Ann Arbor, Mich., USA.
authors.forename: Erica L
authors.initials: EL
authors.lastname: Scheller
authors.forename: Gina M
authors.initials: GM
authors.lastname: Leinninger
authors.forename: Kurt D
authors.initials: KD
authors.lastname: Hankenson
authors.forename: Martin G
authors.initials: MG
authors.lastname: Myers
authors.forename: Paul H
authors.initials: PH
authors.lastname: Krebsbach
mesh_terms: D000375:Aging / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D024043:Collagen Type II / Q000235:genetics*; D024142:Collagen Type VI / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects; D049452:Green Fluorescent Proteins / Q000378:metabolism; D019426:Integrases / Q000378:metabolism; D020738:Leptin / Q000008:administration & dosage / Q000378:metabolism* / Q000494:pharmacology; D051379:Mice; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics*; D021381:Protein Transport / Q000187:drug effects; D011995:Recombination, Genetic / Q000187:drug effects / Q000235:genetics; D050796:STAT3 Transcription Factor / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D024043:Collagen Type II; D024142:Collagen Type VI; D020738:Leptin; D050796:STAT3 Transcription Factor; D049452:Green Fluorescent Proteins; C045073:Cre recombinase; D019426:Integrases