title: Glycoprotein labeling using engineered variants of galactose oxidase obtained by directed evolution.
abstract: A directed evolution approach has been used for the generation of variants of galactose oxidase (GOase) that can selectively oxidize glycans on glycoproteins. The aldehyde function introduced on the glycans D-mannose (Man) and D-N-acetyl glucosamine (GlcNAc) by the enzyme variants could then be used to label the glycoproteins and also whole cells that display mannosides on their surface.
authors.affiliation: School of Chemistry, University of Manchester, Manchester Interdisciplinary Biocentre, Manchester, M1 7DN, UK.
authors.forename: Julie B
authors.initials: JB
authors.lastname: Rannes
authors.forename: Avgousta
authors.initials: A
authors.lastname: Ioannou
authors.forename: Simon C
authors.initials: SC
authors.lastname: Willies
authors.forename: Gideon
authors.initials: G
authors.lastname: Grogan
authors.forename: Carsten
authors.initials: C
authors.lastname: Behrens
authors.forename: Sabine L
authors.initials: SL
authors.lastname: Flitsch
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Turner
mesh_terms: D002863:Chromogenic Compounds / Q000737:chemistry*; D019143:Evolution, Molecular; D005692:Galactose Oxidase / Q000737:chemistry / Q000235:genetics*; D014644:Genetic Variation; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics*; D008958:Models, Molecular; D015394:Molecular Structure; D015202:Protein Engineering; D013379:Substrate Specificity
chemical_list: D002863:Chromogenic Compounds; D006023:Glycoproteins; D005692:Galactose Oxidase