title: Straightforward, one-step fabrication of ultrathin thermoresponsive films from commercially available pNIPAm for cell culture and recovery.
abstract: The use of thermoresponsive surfaces as platforms for cell culture and cell regeneration has been explored over the last couple of decades. Poly-N-isopropylacrylamide (pNIPAm) is a well characterized thermoresponsive polymer which has an aqueous lower critical solution temperature (LCST) in a physiologically useful range, which allows it to reversibly attract (T < 32 °C) and repel water (T > 32 °C). It is this phenomenon that is exploited in temperature-controlled cell harvesting. pNIPAm coatings are generally poorly cell compatible and a number of complex or expensive techniques have been developed in order to overcome this issue. This study seeks to design a simple one-step system whereby commercially sourced pNIPAm is used to achieve similar results. Films were deposited using the operationally simple but rheologically complex spin coating technique. Reversible temperature modulated cell adhesion was achieved using a variety of different cell lines. This system offers a simplistic and cheaper alternative to methods used elsewhere.
authors.affiliation: School of Chemistry, National University of Ireland Galway, Galway, Ireland. nash.maria@gmail.com
authors.forename: Maria E
authors.initials: ME
authors.lastname: Nash
authors.forename: William M
authors.initials: WM
authors.lastname: Carroll
authors.forename: Natalia
authors.initials: N
authors.lastname: Nikoloskya
authors.forename: Rongbing
authors.initials: R
authors.lastname: Yang
authors.forename: Claire
authors.initials: C
authors.lastname: O'Connell
authors.forename: Alexander V
authors.initials: AV
authors.lastname: Gorelov
authors.forename: Peter
authors.initials: P
authors.lastname: Dockery
authors.forename: Catherine
authors.initials: C
authors.lastname: Liptrot
authors.forename: Fiona M
authors.initials: FM
authors.lastname: Lyng
authors.forename: Amaya
authors.initials: A
authors.lastname: Garcia
authors.forename: Yury A
authors.initials: YA
authors.lastname: Rochev
mesh_terms: D016475:3T3 Cells; D000180:Acrylic Resins / Q000737:chemistry*; D000818:Animals; D002448:Cell Adhesion; D051379:Mice; D011108:Polymers / Q000737:chemistry*; D013696:Temperature
chemical_list: D000180:Acrylic Resins; D011108:Polymers; C052970:poly-N-isopropylacrylamide