title: Intravital microscopy of subpleural alveoli via transthoracic endoscopy.
abstract: Transfer of too high mechanical energy from the ventilator to the lung's alveolar tissue is the main cause for ventilator-induced lung injury (VILI). To investigate the effects of cyclic energy transfer to the alveoli, we introduce a new method of transthoracic endoscopy that provides morphological as well as functional information about alveolar geometry and mechanics. We evaluate the new endoscopic method to continuously record images of focused subpleural alveoli. The method is evaluated by using finite element modeling techniques and by direct observation of subpleural alveoli both in isolated rat lungs as well as in intact animals (rats). The results confirm the overall low invasiveness of the endoscopic method insofar as the mechanical influences on the recorded alveoli are only marginal. It is, hence, a suited method for intravital microscopy in the rat model as well as in larger animals.
authors.affiliation: Division of Experimental Anaesthesiology, University Medical Center Freiburg, Hugstetter Strasse 55, 79106 Freiburg, Germany. David.Schwenninger@uniklinik-freiburg.de
authors.forename: David
authors.initials: D
authors.lastname: Schwenninger
authors.forename: Hanna
authors.initials: H
authors.lastname: Runck
authors.forename: Stefan
authors.initials: S
authors.lastname: Schumann
authors.forename: Jörg
authors.initials: J
authors.lastname: Haberstroh
authors.forename: Sven
authors.initials: S
authors.lastname: Meissner
authors.forename: Edmund
authors.initials: E
authors.lastname: Koch
authors.forename: Josef
authors.initials: J
authors.lastname: Guttmann
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D018715:Microscopy, Video / Q000379:methods*; D010313:Partial Pressure; D011650:Pulmonary Alveoli / Q000166:cytology* / Q000293:injuries / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D020708:Thoracoscopes; D013906:Thoracoscopy / Q000295:instrumentation / Q000379:methods*; D041623:Tomography, Optical Coherence / Q000379:methods*