title: Jeotgalibacillus soli sp. nov., a Gram-stain-positive bacterium isolated from soil.
abstract: A Gram-staining-positive, motile, rod-shaped, spore-forming bacterium, designated P9(T), was isolated from soil in Portugal. This organism was aerobic and catalase- and oxidase-positive. It had an optimum growth temperature of about 35 °C and an optimum growth pH of about 8.0-8.5, and grew in medium with 0-9% (w/v) NaCl. The cell-wall peptidoglycan was of the A1α type, with L-lysine as the diagnostic diamino acid. The major respiratory quinone was menaquinone 7 (MK-7) and the major fatty acids were anteiso-C(15:0) (45.4%), iso-C(15:0) (22.0%) and anteiso-C(17:0) (11.2%). The genomic DNA G+C content was about 39.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain P9(T) was most closely related to Jeotgalibacillus campisalis DSM 18983(T) (96.8%) and Jeotgalibacillus marinus DSM 1297(T) (96.5%). These two recognized species formed a coherent cluster with strain P9(T) that was supported by a bootstrap value of 99%. On the basis of the phylogenetic analysis and physiological and biochemical characteristics, strain P9(T) (=DSM 23228(T)=LMG 25523(T)) represents a novel species of the genus Jeotgalibacillus, for which the name Jeotgalibacillus soli sp. nov. is proposed.
authors.affiliation: Department of Life Sciences, Apartado 3046, University of Coimbra, 3001-401 Coimbra, Portugal, and Center for Neuroscience and Cell Biology, University of Coimbra, 3004-517 Coimbra, Portugal.
authors.forename: Sofia
authors.initials: S
authors.lastname: Cunha
authors.affiliation: Department of Life Sciences, Apartado 3046, University of Coimbra, 3001-401 Coimbra, Portugal, and Center for Neuroscience and Cell Biology, University of Coimbra, 3004-517 Coimbra, Portugal.
authors.forename: Igor
authors.initials: I
authors.lastname: Tiago
authors.affiliation: Department of Life Sciences, Apartado 3046, University of Coimbra, 3001-401 Coimbra, Portugal, and Center for Neuroscience and Cell Biology, University of Coimbra, 3004-517 Coimbra, Portugal.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Paiva
authors.affiliation: Department of Life Sciences, Apartado 3046, University of Coimbra, 3001-401 Coimbra, Portugal, and Center for Neuroscience and Cell Biology, University of Coimbra, 3004-517 Coimbra, Portugal.
authors.forename: Fernanda
authors.initials: F
authors.lastname: Nobre
authors.affiliation: Department of Life Sciences, Apartado 3046, University of Coimbra, 3001-401 Coimbra, Portugal, and Center for Neuroscience and Cell Biology, University of Coimbra, 3004-517 Coimbra, Portugal.
authors.forename: Milton S
authors.initials: MS
authors.lastname: da Costa
authors.affiliation: Department of Life Sciences, Apartado 3046, University of Coimbra, 3001-401 Coimbra, Portugal, and Center for Neuroscience and Cell Biology, University of Coimbra, 3004-517 Coimbra, Portugal.
authors.forename: António
authors.initials: A
authors.lastname: Veríssimo
mesh_terms: D000332:Aerobiosis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D016227:Benzoquinones / Q000032:analysis; D002374:Catalase / Q000378:metabolism; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008239:Lysine / Q000032:analysis; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000378:metabolism; D010457:Peptidoglycan / Q000737:chemistry; D010802:Phylogeny; D058641:Planococcaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D011174:Portugal; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D012988:Soil Microbiology; D013696:Temperature
chemical_list: D016227:Benzoquinones; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; C004532:quinone; D012965:Sodium Chloride; D010088:Oxidoreductases; D002374:Catalase; D008239:Lysine