title: One-to-one quantum dot-labeled single long DNA probes.
abstract: Quantum dots (QDs) have been received most attention due to their unique properties. Constructing QDs conjugated with certain number of biomolecules is considered as one of the most important research goals in nanobiotechnology. In this study, we report polymerase chain reaction (PCR) amplification of primer oligonucleotides bound to QDs, termed as QD-based PCR. Characterization of QD-based PCR products by gel electrophoresis and atomic force microscopy showed that QD-labeled long DNA strands were synthesized and only a single long DNA strand was conjugated with a QD. The QD-based PCR products still kept fluorescence properties. Moreover, the one-to-one QD-labeled long DNA conjugates as probes could detect a single-copy gene on maize chromosomes by fluorescence in situ hybridization. Labeling a single QD to a single long DNA will make detection of small single-copy DNA fragments, quantitative detection and single molecule imaging come true by nanotechnology, and it will promote medical diagnosis and basic biological research as well as nano-material fabrication.
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, PR China.
authors.forename: Shibin
authors.initials: S
authors.lastname: He
authors.forename: Bi-Hai
authors.initials: BH
authors.lastname: Huang
authors.forename: Junjun
authors.initials: J
authors.lastname: Tan
authors.forename: Qing-Ying
authors.initials: QY
authors.lastname: Luo
authors.forename: Yi
authors.initials: Y
authors.lastname: Lin
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Yong
authors.initials: Y
authors.lastname: Hu
authors.forename: Lu
authors.initials: L
authors.lastname: Zhang
authors.forename: Shihan
authors.initials: S
authors.lastname: Yan
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.forename: Dai-Wen
authors.initials: DW
authors.lastname: Pang
authors.forename: Lijia
authors.initials: L
authors.lastname: Li
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D000445:Aldehyde Oxidoreductases / Q000235:genetics; D000588:Amines / Q000737:chemistry; D019187:Cadmium Compounds / Q000737:chemistry; D032461:Chromosomes, Plant / Q000235:genetics; D015342:DNA Probes / Q000737:chemistry* / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D017404:In Situ Hybridization, Fluorescence; D008027:Light; D018625:Microscopy, Atomic Force; D046529:Microscopy, Electron, Transmission; D009841:Oligonucleotides / Q000737:chemistry; D010316:Particle Size; D016133:Polymerase Chain Reaction; D045663:Quantum Dots; D012542:Scattering, Radiation; D018036:Selenium Compounds / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013194:Staining and Labeling / Q000379:methods*; D013440:Sulfides / Q000737:chemistry; D013696:Temperature; D003313:Zea mays / Q000235:genetics; D017967:Zinc Compounds / Q000737:chemistry
chemical_list: D000180:Acrylic Resins; D000588:Amines; D019187:Cadmium Compounds; D015342:DNA Probes; D009841:Oligonucleotides; D018036:Selenium Compounds; D013440:Sulfides; D017967:Zinc Compounds; C006903:carbopol 940; C058667:cadmium selenide; D000445:Aldehyde Oxidoreductases; C038828:long-chain-aldehyde dehydrogenase; C031238:zinc sulfide; C008699:octylamine