title: Structural identification of the metabolites for strictosamide in rats bile by an ion trap-TOF mass spectrometer and mass defect filter technique.
abstract: We report herein, a facile metabolite identification workflow on the antimicrobial strictosamide, which is derived from accurate mass measurement by a hybrid ion trap-TOF mass spectrometer. In step 1, the parent drug and metabolites in rat bile were separated on an HPLC column followed by ion trap-TOF mass spectrometer analysis after a single oral dose of 50mg/kg strictosamide. In step 2, mass defect filter technique, which enables high-resolution mass spectrometers to be utilized for detecting drug metabolites based on well-defined mass defect ranges, was used to find metabolites in the mass spectrum. In step 3, the differences of accurate masses and their mass fragmentation pattern among the parent drug and metabolites used to assign structures for the metabolites successfully. As a result, five metabolites of strictosamide were found in rat bile, and all the metabolites were reported for the first time.
authors.affiliation: Key Lab of Drug Metabolism & Pharmacokinetics, China Pharmaceutical University, No. 24, Tongjiaxiang Street, Nanjing 210009, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liang
authors.forename: Wei
authors.initials: W
authors.lastname: Xiao
authors.forename: Chen
authors.initials: C
authors.lastname: Dai
authors.forename: Lin
authors.initials: L
authors.lastname: Xie
authors.forename: Gang
authors.initials: G
authors.lastname: Ding
authors.forename: Guangji
authors.initials: G
authors.lastname: Wang
authors.forename: Zhaoqing
authors.initials: Z
authors.lastname: Meng
authors.forename: Juan
authors.initials: J
authors.lastname: Zhang
authors.forename: An
authors.initials: A
authors.lastname: Kang
authors.forename: Tong
authors.initials: T
authors.lastname: Xie
authors.forename: Yanna
authors.initials: Y
authors.lastname: Liu
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Zhou
authors.forename: Wenjun
authors.initials: W
authors.lastname: Liu
authors.forename: Li
authors.initials: L
authors.lastname: Zhao
authors.forename: Jia
authors.initials: J
authors.lastname: Xu
mesh_terms: D000818:Animals; D001646:Bile / Q000737:chemistry* / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014748:Vinca Alkaloids / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D014748:Vinca Alkaloids; C539462:strictosamide