title: The laparoscopy splenic injury repair: the use of fibrin glue in a heparinized porcine model.
abstract: OBJECTIVE
  To investigate the effectiveness of fibrin glue (laparoscopic via) into promote the hemostasis of a spleen injury on a heparinized porcine model.
  
  
  METHODS
  Eighteen Landrace porcine were submitted to laparoscopic spleen injury and randomly distributed: GHA (heparin plus adhesive), GH (heparin without adhesive) and GS (Sham - without heparin or adhesive). Ten minutes before the surgical procedures a single IV dose (200UI/kg) of heparin sodium was administrated only to groups GHA and GH. In the GHA, adhesive was applied after the mechanical injury and recorded the time until the polymerization and clot formation.
  
  
  RESULTS
  No significant differences occurred among the groups (Fisher test) considering the weight and surgery time. The blood amount in the abdominal cavity on GH was significantly higher in comparison to the sham group and especially with the GHA (p<0.004). No significant differences were observed in the body temperature, heart rate, cardiac output, means arterial pressure, pulmonary artery pressure during the experiment. The activated partial thromboplastin time (APTT) was lower in the GHA in comparison to GH (p<0.003).
  
  
  CONCLUSIONS
  The fibrin biological adhesive applied by laparoscopy is effective for hemostasis of minor spleen injury in a porcine model under the effect of anticoagulant drug.
authors.affiliation: Surgery Residence, County Hospital, Campo Limpo, Sao Paulo, Brazil. dalmerfreire@gmail.com
authors.forename: Dalmer Faria
authors.initials: DF
authors.lastname: Freire
authors.forename: Murched Omar
authors.initials: MO
authors.lastname: Taha
authors.forename: José Homero
authors.initials: JH
authors.lastname: Soares
authors.forename: Manoel de Jesus
authors.initials: Mde J
authors.lastname: Simões
authors.forename: Anna Luiza Negrini
authors.initials: AL
authors.lastname: Fagundes
authors.forename: Djalma José
authors.initials: DJ
authors.lastname: Fagundes
mesh_terms: D000818:Animals; D015718:Fibrin Tissue Adhesive / Q000627:therapeutic use*; D006488:Hemostasis, Surgical / Q000379:methods*; D010535:Laparoscopy; D008297:Male; D011897:Random Allocation; D013154:Spleen / Q000293:injuries* / Q000601:surgery*; D013552:Swine; D014014:Tissue Adhesives / Q000627:therapeutic use*
chemical_list: D015718:Fibrin Tissue Adhesive; D014014:Tissue Adhesives