title: Optimization of polymerization parameters for the sorption of oseltamivir onto molecularly imprinted polymers.
abstract: Molecularly imprinted polymers (MIPs) are tailor-made polymers with high selectivity for a given analyte, or group of structurally related compounds. The influence of the process parameters (the moles of functional monomer and cross-linker, the selection of functional monomer and solvent) on the preparation of oseltamivir (OS)-imprinted polymers was investigated. A mathematical method for uniform design to optimize these selected parameters and to increase the MIP selectivity for template molecules was applied. The optimal conditions to synthesize MIP were 0.69 mmol 30% acrylamide (AA) + 70% 4-Vinylpyridine (4-VP) and 5.0 mmol ethylene glycol dimethacrylate (EGDMA) copolymerized in 5 ml toluene in the presence of 0.1 mmol OS. MIP showed high affinity and selectivity for separation of the template molecule from other compounds. In the present study, we have established an effective LC-MS/MS method to identify and quantify OS with good sensitivity, accuracy and precision.
authors.affiliation: Key Laboratory of New Animal Drug Project, Gansu Province, Key Laboratory of Veterinary Pharmaceutics Discovery, Ministry of Agriculture, Chinese Academy of Agricultural Sciences, Xiaoxihu, Qilihe District, Lanzhou, China.
authors.forename: Yajun
authors.initials: Y
authors.lastname: Yang
authors.forename: Jianyong
authors.initials: J
authors.lastname: Li
authors.forename: Yurong
authors.initials: Y
authors.lastname: Liu
authors.forename: Jiyu
authors.initials: J
authors.lastname: Zhang
authors.forename: Bing
authors.initials: B
authors.lastname: Li
authors.forename: Xuepeng
authors.initials: X
authors.lastname: Cai
mesh_terms: D000327:Adsorption; D000998:Antiviral Agents; D054802:Molecular Imprinting / Q000379:methods*; D053139:Oseltamivir / Q000032:analysis* / Q000302:isolation & purification; D058105:Polymerization; D011108:Polymers / Q000138:chemical synthesis*; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry
chemical_list: D000998:Antiviral Agents; D011108:Polymers; D053139:Oseltamivir