title: Hydrolysis of Chlorella by Cellulomonas sp. YJ5 cellulases and its biofunctional properties.
abstract: Both 10% and 20% (w/w) Chlorella suspensions were hydrolyzed by 150 to 350 U/mL of cellulases from a 3-d cultivation of Cellulomonas sp. YJ5. Higher chlorophyll, reducing sugars and soluble proteins, and lower residual insoluble solid were observed on both samples after 30-min hydrolysis by various concentrations of cellulases at 50 °C. Decrease in insoluble solid, increases in soluble proteins, peptides and chlorophyll contents, and microscopic observation indicated obvious lysis of cell walls occurred during 60- to 180-min hydrolysis. Significant increases in soluble proteins, peptides, Fe(2+) chelating ability, trolox equivalent antioxidation capacity (TEAC), and reducing power was obtained after 3-h hydrolysis by 150 U/mL of cellulase. These data suggested that cellulolysis technology has high application potential in Chlorella industry.
authors.affiliation: Dept. of Seafood Science, Natl. Kaohsiung Marine Univ., No.142 Hai-Chuan Rd. Nan-Tzu, Kaohsiung 81143, Taiwan. ljyin@mail.nkmu.edu.tw
authors.forename: Li-Jung
authors.initials: LJ
authors.lastname: Yin
authors.forename: Shann-Tzong
authors.initials: ST
authors.lastname: Jiang
authors.forename: Shen-Hwei
authors.initials: SH
authors.lastname: Pon
authors.forename: Hsin-Hung
authors.initials: HH
authors.lastname: Lin
mesh_terms: D000975:Antioxidants / Q000032:analysis; D002241:Carbohydrates / Q000032:analysis; D044602:Cellulases / Q000378:metabolism*; D040143:Cellulomonas / Q000201:enzymology*; D002708:Chlorella / Q000378:metabolism*; D002734:Chlorophyll / Q000032:analysis; D006868:Hydrolysis; D011506:Proteins / Q000032:analysis
chemical_list: D000975:Antioxidants; D002241:Carbohydrates; D011506:Proteins; D002734:Chlorophyll; D044602:Cellulases