title: Determination of puerarin in biological samples and its application to a pharmacokinetic study by flow-injection chemiluminescence.
abstract: A simple and sensitive flow injection-chemiluminescence (FI-CL) method has been developed for the determination of puerarin, based on the fact that puerarin can greatly inhibit CL of the luminol-H₂O₂-haemoglobin system. The inhibition of CL intensity was linear to the logarithm of the concentration of puerarin in the range 0.08-10.0 μg/mL (r²  = 0.9912). The limit of detection was 0.05 μg/mL (3σ) and the relative standard deviation (RSD) for 1.0 μg/mL (n = 11) of puerarin solution was 1.4%. Coupled with solid-phase extraction (SPE) as the sample pretreatment, the determination of puerarin in biological samples and a preliminary pharmocokinetic study of puerarin in rats were performed. The recoveries for plasma and urine at three different concentrations were 89.2-110.0% and 91.4-104.8%, respectively. The pharmacokinetics of puerarin in plasma of rat coincides with the two-compartment open model. The T(½α) , T(½β) , CL/F, V(Z/F), AUC(₀₋t), MRT₀₋∞, T(max) and C(max) were 0.77 ± 0.21 h, 7.55 ± 2.64 h, 2.43 ± 1.02 L/kg/h, 11.40 ± 3.45 L/kg, 56.67 ± 10.65 mg/h/L, 5.04 ± 2.78 h, 1.00 ± 0.35 h and 19.70 ± 4.67 μg/mL, respectively.
authors.affiliation: Department of Chemistry, Zhengzhou University, Zhengzhou 450001, People's Republic of China.
authors.forename: Ran
authors.initials: R
authors.lastname: Yang
authors.forename: Qingwen
authors.initials: Q
authors.lastname: Wang
authors.forename: Huajin
authors.initials: H
authors.lastname: Zeng
authors.forename: Zao
authors.initials: Z
authors.lastname: Qin
authors.forename: Jianjun
authors.initials: J
authors.lastname: Li
authors.forename: Lingbo
authors.initials: L
authors.lastname: Qu
mesh_terms: D000818:Animals; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000493:pharmacokinetics; D017022:Flow Injection Analysis / Q000379:methods*; D006801:Humans; D007529:Isoflavones / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics / Q000652:urine; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000379:methods*; D008297:Male; D029908:Pueraria / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004365:Drugs, Chinese Herbal; D007529:Isoflavones; C033607:puerarin