title: Immunohistochemical detection of CDKN2, retinoblastoma and p53 gene products in primary astrocytic tumors.
abstract: The expressions of p16(INK4), retinoblastoma (RB) and p53 protein were immunohistochemically examined in 70 primary astrocytic tumors. In 58 patients with high grade astrocytoma (18 anaplastic astrocytomas and 40 glioblastomas), 30 (51.1%) and 15 (25.9%) cases were undetectable for p16(INK4) and pRB, respectively, but their lack occurred infrequently in 12 low grade astrocytomas. The expression of p16(INK4) was inversely correlated with that of PRB, especially in glioblastomas. Accumulation of p53 was detected in 32 (45.7%) of 70 cases without any dependence on the grade. A deregulation of three tumor suppressor gene products most often occurs singly. Only patients with negative staining for pRB were significantly associated with a shorter survival time. Our findings suggest that loss of functional pRB at the G1/S check point may represent an important step in glioblastoma development and have a stronger negative impact on clinical outcome than p16(INK4) or p53 aberrations.
authors.affiliation: NARA MED UNIV,DEPT PATHOL 2,KASHIHARA,NARA 634,JAPAN. UNIV OSAKA PREFECTURE,COLL INTEGRATED ARTS & SCI,DEPT LIFE SCI,SAKAI,OSAKA 593,JAPAN.
authors.forename: M
authors.initials: M
authors.lastname: Nakamura
authors.forename: N
authors.initials: N
authors.lastname: Konishi
authors.forename: Y
authors.initials: Y
authors.lastname: Hiasa
authors.forename: S
authors.initials: S
authors.lastname: Tsunoda
authors.forename: Y
authors.initials: Y
authors.lastname: Fukushima
authors.forename: T
authors.initials: T
authors.lastname: Tsuzuki
authors.forename: K
authors.initials: K
authors.lastname: Takemura
authors.forename: H
authors.initials: H
authors.lastname: Aoki
authors.forename: K
authors.initials: K
authors.lastname: Kobitsu
authors.forename: T
authors.initials: T
authors.lastname: Sakaki