title: Comparative physiology and transcriptional networks underlying the heat shock response in Populus trichocarpa, Arabidopsis thaliana and Glycine max.
abstract: The heat shock response continues to be layered with additional complexity as interactions and crosstalk among heat shock proteins (HSPs), the reactive oxygen network and hormonal signalling are discovered. However, comparative analyses exploring variation in each of these processes among species remain relatively unexplored. In controlled environment experiments, photosynthetic response curves were conducted from 22 to 42 °C and indicated that temperature optimum of light-saturated photosynthesis was greater for Glycine max relative to Arabidopsis thaliana or Populus trichocarpa. Transcript profiles were taken at defined states along the temperature response curves, and inferred pathway analysis revealed species-specific variation in the abiotic stress and the minor carbohydrate raffinose/galactinol pathways. A weighted gene co-expression network approach was used to group individual genes into network modules linking biochemical measures of the antioxidant system to leaf-level photosynthesis among P. trichocarpa, G. max and A. thaliana. Network-enabled results revealed an expansion in the G. max HSP17 protein family and divergence in the regulation of the antioxidant and heat shock modules relative to P. trichocarpa and A. thaliana. These results indicate that although the heat shock response is highly conserved, there is considerable species-specific variation in its regulation.
authors.affiliation: Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA. westondj@ornl.gov
authors.forename: David J
authors.initials: DJ
authors.lastname: Weston
authors.forename: Abhijit A
authors.initials: AA
authors.lastname: Karve
authors.forename: Lee E
authors.initials: LE
authors.lastname: Gunter
authors.forename: Sara S
authors.initials: SS
authors.lastname: Jawdy
authors.forename: Xiaohan
authors.initials: X
authors.lastname: Yang
authors.forename: Sara M
authors.initials: SM
authors.lastname: Allen
authors.forename: Stan D
authors.initials: SD
authors.lastname: Wullschleger
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D017360:Arabidopsis / Q000235:genetics / Q000502:physiology*; D005075:Biological Evolution; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D053263:Gene Regulatory Networks / Q000235:genetics / Q000502:physiology*; D017343:Genes, Plant / Q000235:genetics; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D018869:Heat-Shock Response / Q000502:physiology*; D006706:Homeostasis; D008027:Light; D010788:Photosynthesis / Q000502:physiology; D010802:Phylogeny; D018515:Plant Leaves / Q000235:genetics / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018526:Plant Transpiration; D032107:Populus / Q000235:genetics / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction; D013025:Glycine max / Q000235:genetics / Q000502:physiology*; D013045:Species Specificity; D049490:Systems Biology; D013696:Temperature
chemical_list: D000975:Antioxidants; D006360:Heat-Shock Proteins; D010940:Plant Proteins; D017382:Reactive Oxygen Species