title: Hepatoprotective effect of curcumin in lipopolysaccharide/-galactosamine model of liver injury in rats: relationship to HO-1/CO antioxidant system.
abstract: This work studied a relationship between HO-1/CO system and lipid peroxidation with consequent effects on liver functions and NOS-2. We focused on curcumin pretreatment in rat toxic model of d-galactosamine and lipopolysaccharide. Hepatocyte viability, lipid peroxidation, antioxidant status, ALT and AST were evaluated. HO-1 and NOS-2 expressions and respective enzyme activity were determined. Curcumin caused decreases in ALT and AST levels as well as in lipid peroxidation. Furthermore, curcumin pretreatment increased liver HO-1 (2.4-fold, p=0.001), but reduced NOS-2 (4.1-fold, p=0.01) expressions. In conclusion, the tuning of CO/NO pathways is important in shedding light on curcumin's cytoprotective effects in this model.
authors.affiliation: Institute of Pharmacology, Charles University in Prague, Czech Republic. dcern@lf1.cuni.cz
authors.forename: Dalibor
authors.initials: D
authors.lastname: Cerný
authors.forename: Nataša
authors.initials: N
authors.lastname: Lekić
authors.forename: Kateřina
authors.initials: K
authors.lastname: Váňová
authors.forename: Lucie
authors.initials: L
authors.lastname: Muchová
authors.forename: Aleš
authors.initials: A
authors.lastname: Hořínek
authors.forename: Eva
authors.initials: E
authors.lastname: Kmoníčková
authors.forename: Zdeněk
authors.initials: Z
authors.lastname: Zídek
authors.forename: Ludmila
authors.initials: L
authors.lastname: Kameníková
authors.forename: Hassan
authors.initials: H
authors.lastname: Farghali
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D001219:Aspartate Aminotransferases / Q000097:blood; D002248:Carbon Monoxide / Q000378:metabolism*; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy* / Q000378:metabolism; D030024:Curcuma / Q000737:chemistry; D003474:Curcumin / Q000494:pharmacology / Q000627:therapeutic use*; D004195:Disease Models, Animal; D005688:Galactosamine; D051547:Heme Oxygenase-1 / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects; D008070:Lipopolysaccharides; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction
chemical_list: D000975:Antioxidants; D008070:Lipopolysaccharides; D010936:Plant Extracts; D005688:Galactosamine; D002248:Carbon Monoxide; D052247:Nitric Oxide Synthase Type II; D051547:Heme Oxygenase-1; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D003474:Curcumin