title: Development of an immunochromatographic strip test for rapid detection of melamine in raw milk, milk products and animal feed.
abstract: A simple, rapid and sensitive immunogold chromatographic strip test based on a monoclonal antibody was developed for the detection of melamine (MEL) residues in raw milk, milk products and animal feed. The limit of detection was estimated to be 0.05 μg/mL in raw milk, since the detection test line on the strip test completely disappeared at this concentration. The limit of detection was 2 μg/mL (or 2 μg/g) for milk drinks, yogurt, condensed milk, cheese, and animal feed and 1 μg/g for milk powder. Sample pretreatment was simple and rapid, and the results can be obtained within 3-10 min. A parallel analysis of MEL in 52 blind raw milk samples conducted by gas chromatography-mass spectrometry showed comparable results to those obtained from the strip test. The results demonstrate that the developed method is suitable for the onsite determination of MEL residues in a large number of samples.
authors.affiliation: Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, People's Republic of China.
authors.forename: Xiangmei
authors.initials: X
authors.lastname: Li
authors.forename: Pengjie
authors.initials: P
authors.lastname: Luo
authors.forename: Shusheng
authors.initials: S
authors.lastname: Tang
authors.forename: Ross C
authors.initials: RC
authors.lastname: Beier
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Wu
authors.forename: Lili
authors.initials: L
authors.lastname: Yang
authors.forename: Yanwei
authors.initials: Y
authors.lastname: Li
authors.forename: Xilong
authors.initials: X
authors.lastname: Xiao
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D002845:Chromatography / Q000379:methods*; D003611:Dairy Products / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D007118:Immunoassay / Q000379:methods*; D008892:Milk / Q000737:chemistry*; D014227:Triazines / Q000032:analysis*
chemical_list: D014227:Triazines; C011907:melamine