title: Structural study of prepuce in hypospadias--does topical treatment with testosterone produce alterations in prepuce vascularization?
abstract: OBJECTIVE
  Androgen stimulation before hypospadias surgery has resulted in increased penile size, fewer complications and improved cosmesis, and suggests increased neovascularization. To our knowledge the real effect on neovascularization remains to be proved. We studied the histological effects of testosterone on neovascularization.
  
  
  METHODS
  A total of 26 boys with hypospadias were randomly allocated to 2 groups before surgical correction. Group 1 did not receive any treatment and group 2 received 1% testosterone propionate ointment twice daily for 30 days before surgery. During the surgical procedure a fragment of prepuce was excised and prepared for histological evaluation. The number and volume density of blood vessels were determined by labeling for von Willebrand's factor. Blood vessel quantification as volume density was done using a video microscopy system with a superimposed cycloid arch test system.
  
  
  RESULTS
  The groups were similar in age and hypospadias classification. Testosterone treated prepuces (group 2) had an increased absolute number of blood vessels (mean ± SD 8.5 ± 1.3 vs 4.8 ± 1.8 vessels per field) and increased blood vessel volume density (mean 50.5% ± 7.8% vs 24.8% ± 8.6% vessels per point) (each p <0.001) compared to those in untreated patients (group 1).
  
  
  CONCLUSIONS
  The use of 1% testosterone propionate ointment before hypospadias surgery produces neovascularization in absolute numbers and in volume density.
authors.affiliation: Federal University of Juiz de Fora, Rio Branco 2985/sl.605, Juiz de Fora-MG, Brazil. jmbnetto@uropedjf.com.br
authors.forename: André Netto
authors.initials: AN
authors.lastname: Bastos
authors.forename: Leonardo Resende Souza
authors.initials: LR
authors.lastname: Oliveira
authors.forename: Carlos Eduardo Prata Fernandes
authors.initials: CE
authors.lastname: Ferrarez
authors.forename: André Avarese
authors.initials: AA
authors.lastname: de Figueiredo
authors.forename: Luciano Alves
authors.initials: LA
authors.lastname: Favorito
authors.forename: Jose Murillo
authors.initials: JM
authors.lastname: Bastos Netto
mesh_terms: D000287:Administration, Topical; D002675:Child, Preschool; D052816:Foreskin / Q000098:blood supply* / Q000187:drug effects*; D006801:Humans; D007021:Hypospadias / Q000601:surgery*; D007223:Infant; D008297:Male; D013739:Testosterone / Q000008:administration & dosage* / Q000494:pharmacology
chemical_list: D013739:Testosterone