title: Phosphorylation of the epidermal growth factor receptor (EGFR) is essential for interleukin-8 release from intestinal epithelial cells in response to challenge with Escherichia coli O157 : H7 flagellin.
abstract: Enterohaemorrhagic Escherichia coli O157 : H7 is a major foodborne and environmental pathogen responsible for both sporadic cases and outbreaks of food poisoning, which can lead to serious sequelae, such as haemolytic uraemic syndrome. The structural subunit of E. coli O157 : H7 flagella is flagellin, which is both the antigenic determinant of the H7 serotype, an important factor in colonization, and an immunomodulatory protein that has been determined to be a major pro-inflammatory component through the instigation of host cell signalling pathways. Flagellin has highly conserved N- and C-terminal regions that are recognized by the host cell pattern recognition receptor Toll-like receptor (TLR) 5. Activation of this receptor triggers cell signalling cascades, which are known to activate host cell kinases and transcription factors that respond with the production of inflammatory mediators such as the chemokine interleukin-8 (IL-8), although the exact components of this pathway are not yet fully characterized. We demonstrate that E. coli O157 : H7-derived flagellin induces rapid phosphorylation of the epidermal growth factor receptor (EGFR), as an early event in intestinal epithelial cell signalling, and that this is required for the release of the pro-inflammatory cytokine IL-8.
authors.affiliation: Biomedical Sciences and Microbiology Group, School of Life, Sport and Social Sciences, Faculty of Health, Life and Social Sciences, Sighthill Campus, Edinburgh Napier University, Edinburgh EH11 4BN, UK.
authors.forename: Douglas J
authors.initials: DJ
authors.lastname: Fraser-Pitt
authors.affiliation: Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, Midlothian EH26 0PZ, UK.
authors.forename: Pamela
authors.initials: P
authors.lastname: Cameron
authors.affiliation: Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, Midlothian EH26 0PZ, UK.
authors.forename: Tom N
authors.initials: TN
authors.lastname: McNeilly
authors.affiliation: Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, Midlothian EH26 0PZ, UK.
authors.forename: Amanda
authors.initials: A
authors.lastname: Boyd
authors.affiliation: Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, Midlothian EH26 0PZ, UK.
authors.forename: Erin D T
authors.initials: EDT
authors.lastname: Manson
authors.affiliation: Institute for Comparative Medicine, Faculty of Veterinary Medicine, University of Glasgow, Glasgow G61 1QH, UK.|Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, Midlothian EH26 0PZ, UK.
authors.forename: David G E
authors.initials: DGE
authors.lastname: Smith
mesh_terms: D018938:Caco-2 Cells; D004847:Epithelial Cells / Q000276:immunology*; D066246:ErbB Receptors / Q000378:metabolism*; D019453:Escherichia coli O157 / Q000276:immunology*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000276:immunology*; D005408:Flagellin / Q000235:genetics / Q000276:immunology*; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000276:immunology*; D010766:Phosphorylation; D015398:Signal Transduction; D051198:Toll-Like Receptor 5 / Q000276:immunology
chemical_list: D029968:Escherichia coli Proteins; C516095:FliC protein, E coli; D016209:Interleukin-8; C495346:TLR5 protein, human; D051198:Toll-Like Receptor 5; D005408:Flagellin; D066246:ErbB Receptors