title: Development of 15 polymorphic microsatellite markers in the Atlantic tarpon (Megalops atlanticus) for capture-recapture studies.
abstract: Fifteen polymorphic microsatellite DNA loci for Atlantic tarpon, Megalops atlanticus, were isolated by using PIMA, a polymerase chain reaction-based technique. The number of alleles at each locus ranged from two to 24 (mean = 7.7) in 65 specimens from Tampa Bay, Florida. Observed and expected heterozygosities ranged from 0.27 to 0.92 (mean = 0.60) and from 0.28 to 0.95 (mean = 0.62), respectively. Genotypes at one locus deviated significantly from Hardy-Weinberg equilibrium. In exact tests for genotypic disequilibrium, there was no evidence of associations between any pair of loci. Overall, loci were well resolved and highly polymorphic, confirming their suitability for DNA fingerprinting applications and other genetic studies.
authors.affiliation: Florida Fish and Wildlife Research Institute, 100 Eighth Avenue SE, Saint Petersburg, Florida 33701-5095, USA.
authors.forename: Seifu
authors.initials: S
authors.lastname: Seyoum
authors.forename: Michael D
authors.initials: MD
authors.lastname: Tringali
authors.forename: Maryanne
authors.initials: M
authors.lastname: Higham