title: c-Jun/AP-1 transcription factor regulates laminin-1-induced neurite outgrowth in human bone marrow mesenchymal stem cells: role of multiple signaling pathways.
abstract: In this study, we demonstrated that laminin-1 (LM-1) induces neurite outgrowth and enhances the expression of neurofilament-L and MAP2 in human bone marrow mesenchymal stem cells (MSCs). The c-Jun transcription factor was strongly activated by LM-1 during neurite induction. Suppression of c-Jun inhibited the expression of the c-Jun target genes α6 integrin and neurofilament-L, resulting in the loss of neurite outgrowth. Additionally, we found that the LM-1-α6 integrin interaction stimulated phosphorylation of FAK, leading to the activation of JNK and Akt. Pharmacological inhibition of these pathways blocked c-Jun activation and neurite outgrowth. Collectively, our findings suggest that c-Jun/AP-1 activity mediated by JNK, PI3K/Akt and ERK pathways is required for LM-1-induced neurite outgrowth in human bone marrow MSCs.
authors.affiliation: National Centre for Cell Science, Ganeshkhind, Pune, India.
authors.forename: S
authors.initials: S
authors.lastname: Mruthyunjaya
authors.forename: Manchanda
authors.initials: M
authors.lastname: Rumma
authors.forename: Godbole
authors.initials: G
authors.lastname: Ravibhushan
authors.forename: Shiras
authors.initials: S
authors.lastname: Anjali
authors.forename: Shastry
authors.initials: S
authors.lastname: Padma
mesh_terms: D001854:Bone Marrow Cells; D006801:Humans; D039503:Integrin alpha6 / Q000378:metabolism; D007797:Laminin / Q000502:physiology*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000648:ultrastructure; D016501:Neurites / Q000648:ultrastructure*; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D016755:Proto-Oncogene Proteins c-jun / Q000235:genetics; D015398:Signal Transduction / Q000502:physiology; D018808:Transcription Factor AP-1 / Q000502:physiology*
chemical_list: D039503:Integrin alpha6; D007797:Laminin; D016755:Proto-Oncogene Proteins c-jun; D018808:Transcription Factor AP-1; C121346:laminin 1; D051057:Proto-Oncogene Proteins c-akt