title: A low frequency variant within the GWAS locus of MTNR1B affects fasting glucose concentrations: genetic risk is modulated by obesity.
abstract: Two common variants (rs1387153, rs10830963) in MTNR1B have been reported to have independent effects on fasting blood glucose (FBG) levels with increased risk to type 2 diabetes (T2D) in recent genome-wide association studies (GWAS). In this investigation, we report the association of these two variants, and an additional variant (rs1374645) within the GWAS locus of MTNR1B with FBG, 2h glucose, insulin resistance (HOMA IR), β-cell function (HOMA B), and T2D in our sample of Asian Sikhs from India. Our cohort comprised 2222 subjects [1201 T2D, 1021 controls]. None of these SNPs was associated with T2D in this cohort. Our data also could not confirm association of rs1387153 and rs10830963 with FBG phenotype. However, upon stratifying data according to body mass index (BMI) (low ≤ 25 kg/m(2) and high > 25 kg/m(2)) in normoglycemic subjects (n = 1021), the rs1374645 revealed a strong association with low FBG levels in low BMI group (β = -0.073, p = 0.002, Bonferroni p = 0.01) compared to the high BMI group (β = 0.015, p = 0.50). We also detected a strong evidence of interaction between rs1374645 and BMI with respect to FBG levels (p = 0.002). Our data provide new information about the significant impact of another MTNR1B variant on FBG levels that appears to be modulated by BMI. Future confirmation on independent datasets and functional studies will be required to define the role of this variant in fasting glucose variation.
authors.affiliation: Department of Pediatrics, Section of Genetics, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.
authors.forename: L F
authors.initials: LF
authors.lastname: Been
authors.forename: J L
authors.initials: JL
authors.lastname: Hatfield
authors.forename: A
authors.initials: A
authors.lastname: Shankar
authors.forename: C E
authors.initials: CE
authors.lastname: Aston
authors.forename: S
authors.initials: S
authors.lastname: Ralhan
authors.forename: G S
authors.initials: GS
authors.lastname: Wander
authors.forename: N K
authors.initials: NK
authors.lastname: Mehra
authors.forename: J R
authors.initials: JR
authors.lastname: Singh
authors.forename: J J
authors.initials: JJ
authors.lastname: Mulvihill
authors.forename: D K
authors.initials: DK
authors.lastname: Sanghera
mesh_terms: D000328:Adult; D000368:Aged; D044466:Asian People / Q000235:genetics; D001786:Blood Glucose / Q000032:analysis*; D015992:Body Mass Index; D015331:Cohort Studies; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics; D005215:Fasting / Q000097:blood; D005260:Female; D005787:Gene Frequency; D056426:Genetic Loci; D055106:Genome-Wide Association Study; D005838:Genotype; D006801:Humans; D007194:India; D007333:Insulin Resistance; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D044122:Receptor, Melatonin, MT1 / Q000235:genetics* / Q000378:metabolism; D044123:Receptor, Melatonin, MT2; D012307:Risk Factors; D017422:Sequence Analysis, DNA
chemical_list: D001786:Blood Glucose; C573871:MTNR1B protein, human; D044122:Receptor, Melatonin, MT1; D044123:Receptor, Melatonin, MT2