title: Long-term storage of surface-adsorbed protein machines.
abstract: The effective and simple long-term storage of complex functional proteins is critical in achieving commercially viable biosensors. This issue is particularly challenging in recently proposed types of nanobiosensors, where molecular-motor-driven transportation substitutes microfluidics and forms the basis for novel detection schemes. Importantly, therefore, we here describe that delicate heavy meromyosin (HMM)-based nanodevices (HMM motor fragments adsorbed to silanized surfaces and actin bound to HMM) fully maintain their function when stored at -20 °C for more than a month. The mechanisms for the excellent preservation of acto-HMM motor function upon repeated freeze-thaw cycles are discussed. The results are important to the future commercial implementation of motor-based nanodevices and are of more general value to the long-term storage of any protein-based bionanodevice.
authors.affiliation: School of Natural Sciences, Linnaeus University, SE-39245 Kalmar, Sweden. nuria.albettorres@lnu.se
authors.forename: Nuria
authors.initials: N
authors.lastname: Albet-Torres
authors.forename: Alf
authors.initials: A
authors.lastname: Månsson
mesh_terms: D000327:Adsorption; D000818:Animals; D002417:Cattle; D003080:Cold Temperature; D004356:Drug Storage; D055767:Immobilized Proteins / Q000737:chemistry* / Q000378:metabolism; D036103:Nanotechnology / Q000295:instrumentation*; D011817:Rabbits; D012821:Silanes / Q000737:chemistry; D013499:Surface Properties; D013997:Time Factors
chemical_list: D055767:Immobilized Proteins; D012821:Silanes