title: Sperm and embryo analysis of similar t(7;10) translocations transmitted in two families.
abstract: OBJECTIVE
  To compare the sperm meiotic segregation profiles in two men from families with similar t(7;10) translocations and determine the frequency of unbalanced sperm and preimplantation embryos in one couple.
  
  
  METHODS
  Analysis of sperm nuclei and blastomeres by fluorescence in situ hybridization (FISH).
  
  
  METHODS
  Research institute.
  
  
  METHODS
  Carriers of balanced translocations t(7;10)(q34;q24) and t(7;10)(q36;q24.3).
  
  
  METHODS
  Multicolor FISH using probes for chromosomes 7, 10, 8, 18, 21, X, and Y on sperm and preimplantation genetic diagnosis (PGD) of blastomeres.
  
  
  METHODS
  Frequencies of meiotic segregation products in sperm and blastomeres and sperm aneuploidy of chromosomes 8, 18, 21, X, and Y.
  
  
  RESULTS
  Similar meiotic segregation patterns, with preferential alternate segregation (50.6% in patient P1, 48.1% in P2) followed by adjacent 1, adjacent 2 and 3:1 segregations, were observed in the sperm of the two carriers. An interchromosomal effect on the sex chromosomes was found when compared with disomy frequencies reported in control donors. The results of preimplantation genetic diagnosis in the first couple are roughly consistent with the sperm analysis results.
  
  
  CONCLUSIONS
  Carriers of similar translocations show similar segregation profiles. The in vitro fertilization method accompanied by preimplantation genetic diagnosis increases the chance of translocation carriers fathering a healthy child.
authors.affiliation: Department of Genetics and Reproduction, Veterinary Research Institute, Brno, Czech Republic. vozdova@vri.cz
authors.forename: Miluse
authors.initials: M
authors.lastname: Vozdova
authors.forename: Eva
authors.initials: E
authors.lastname: Oracova
authors.forename: Petra
authors.initials: P
authors.lastname: Musilova
authors.forename: Katerina
authors.initials: K
authors.lastname: Kasikova
authors.forename: Petra
authors.initials: P
authors.lastname: Prinosilova
authors.forename: Renata
authors.initials: R
authors.lastname: Gaillyova
authors.forename: Jiri
authors.initials: J
authors.lastname: Rubes
mesh_terms: D000328:Adult; D002879:Chromosomes, Human, Pair 10 / Q000235:genetics*; D002897:Chromosomes, Human, Pair 7 / Q000235:genetics*; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D008297:Male; D010375:Pedigree; D019836:Preimplantation Diagnosis / Q000379:methods*; D013094:Spermatozoa / Q000502:physiology*; D014178:Translocation, Genetic / Q000235:genetics*