title: Alphavirus adsorption to mosquito cells as viewed by freeze fracture immunolabeling.
abstract: Sindbis Virus (SV), the prototype alphavirus in the family togaviridae, infects both mammalian and insect cells. The ability of SV to infect cells possessing significantly different biochemical environments suggests that there may be a common mode of entry into each cell type. Previous studies show that up to 4h post infection cells are permeable to small ions and alpha sarcin suggesting that the plasma membrane is compromised as infection takes place. Thin-section electron microscopy has also shown SV to bind to the plasma membrane and lose its electron dense core through a pore like structure developed upon interaction of the virus with the cell surface. Using freeze-fracture replicas, thin-sections and antibody labeling the data presented herein show virus associated with intramembrane particles on mosquito cells. These data suggest that the intramembrane particles associated with SV may be part of the pore structure consisting of virus proteins and cell receptor.
authors.affiliation: Department of Molecular and Structural Biochemistry, NC State University, Raleigh, NC 27695, USA.
authors.forename: Joseph P
authors.initials: JP
authors.lastname: Kononchik
authors.forename: Ricardo
authors.initials: R
authors.lastname: Vancini
authors.forename: Dennis T
authors.initials: DT
authors.lastname: Brown
mesh_terms: D000327:Adsorption; D000524:Alphavirus / Q000737:chemistry / Q000502:physiology* / Q000648:ultrastructure; D000818:Animals; D002462:Cell Membrane / Q000737:chemistry / Q000648:ultrastructure / Q000821:virology; D002478:Cells, Cultured; D009033:Culicidae / Q000821:virology*; D005614:Freeze Fracturing / Q000379:methods*; D007150:Immunohistochemistry / Q000379:methods*