title: Bacteria contributing to behaviour of radiocarbon in sodium acetate.
abstract: An acetate-utilising bacterium was isolated and identified from deionised water that was used for flooding of paddy soils in this study's batch culture experiments. Bacteria in the deionised water samples formed colonies on agar plates containing [1,2-(14)C] sodium acetate, and the autoradiograms showed that all the colonies were positive for (14)C utilisation. Then one of the acetate-utilising bacteria was isolated. The isolate was characterised by phylogenetic analysis, cell morphology, Gram staining and growth at 30 °C. Phylogenetic analysis based on 16S rRNA sequencing showed that the isolate belonged to the genus Burkholderia. The bacterium was gram-negative rods and grew at 30 °C under aerobic conditions. Based on these characteristics, the isolate was identified as Burkholderia gladioli. Because B. gladioli is often found in soil, water and the rhizosphere, attention must be paid to the relationships between bacteria and the behaviour of (14)C to for the safety assessment of geological disposal of transuranic waste.
authors.affiliation: Office of Biospheric Assessment for Waste Disposal, National Institute of Radiological Sciences, 4-9-1 Anagawa, Inage, Chiba 263-8555, Japan. nobu@nirs.go.jp
authors.forename: Nobuyoshi
authors.initials: N
authors.lastname: Ishii
authors.forename: Shigeo
authors.initials: S
authors.lastname: Uchida
mesh_terms: D015373:Bacterial Typing Techniques; D019117:Burkholderia / Q000145:classification* / Q000235:genetics / Q000378:metabolism*; D002250:Carbon Radioisotopes / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D010641:Phenotype; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics; D058441:Rhizosphere; D019346:Sodium Acetate / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D012988:Soil Microbiology; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism
chemical_list: D002250:Carbon Radioisotopes; D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D014874:Water Pollutants, Chemical; D019346:Sodium Acetate