title: The functions of TFIIF during initiation and transcript elongation are differentially affected by phosphorylation by casein kinase 2.
abstract: The RNA polymerase II (pol II) initiation and elongation factor elongation factor TFIIF can be extensively phosphorylated in vivo, although the significance of this modification has not been clear. We now show that phosphorylation of recombinant TFIIF by casein kinase 2 (CK2) reduces or eliminates some of the functions of TFIIF while paradoxically leaving others intact. Phospho-IIF is fully functional in binding to free pol II and is able to support the initiation of transcription. However, the phosphorylated factor does not bind to stalled elongation complexes as measured in a gel mobility shift assay. Significantly, phosphorylation strongly reduces (or for some truncated versions of RAP74, eliminates) stimulation of transcript elongation by TFIIF. Thus, although TFIIF must participate at the initiation of transcription, its ability to continue its association with pol II and stimulate transcript elongation can be specifically regulated by CK2. This is particularly interesting because CK2 is required for initiation at a subset of pol II promoters. Modulation of TFIIF function could be important in controlling promoter-proximal pausing by pol II during the early stage of transcript elongation.
authors.affiliation: Department of Molecular Genetics, Lerner Research Institute, The Cleveland Clinic, Cleveland, Ohio 44195, USA.
authors.forename: Andrea
authors.initials: A
authors.lastname: Újvári
authors.forename: Mahadeb
authors.initials: M
authors.lastname: Pal
authors.forename: Donal S
authors.initials: DS
authors.lastname: Luse
mesh_terms: D047390:Casein Kinase II / Q000737:chemistry* / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D010766:Phosphorylation / Q000502:physiology; D011401:Promoter Regions, Genetic / Q000502:physiology*; D012319:RNA Polymerase II / Q000737:chemistry* / Q000378:metabolism; D019473:Transcription Factors, TFII / Q000737:chemistry* / Q000378:metabolism; D014158:Transcription, Genetic / Q000502:physiology*
chemical_list: D019473:Transcription Factors, TFII; D047390:Casein Kinase II; D012319:RNA Polymerase II; C056604:transcription factor TFIIF