title: Evaluation of the genotoxicity and cytotoxicity of fexofenadine in cultured human peripheral blood lymphocytes.
abstract: Fexofenadine (FXF) is a new non-sedating antihistamine used in the treatment of seasonal allergic rhinitis and chronic idiopathic urticaria. Studies on FXF genotoxicity and cytotoxicity in cultured human peripheral blood lymphocytes have not been reported so far. Therefore, the present study is the first report investigating the genotoxic and cytotoxic effects of FXF in cultured human peripheral blood lymphocytes in vitro. Cultures were treated with FXF at three concentrations (50, 100 and 150 μg/ml) for 24 and 48 h. Endpoints analyzed included: mitotic index (MI), nuclear division index (NDI), chromosomal aberrations (CA) and micronucleus (MN) assay. Mitomycin C (MMC) was used as a positive control. The results of CA and MN assays showed that FXF was not genotoxic at all the concentrations tested, meanwhile MI and NDI results showed dose-dependent decrease and significant differences were found for at least one concentration. In conclusion, the results of this study suggest that FXF has a cytotoxic effect but not genotoxic effect on human peripheral blood lymphocyte cultures. Further cytogenetic studies, especially about the cell cycle kinetics of FXF are required to elucidate the decreases in dividing cells, and biomonitoring studies should also be conducted with patients receiving therapy with this drug.
authors.affiliation: Ordu University, Faculty of Science and Letters, Department of Biology, 52200 Ordu, Turkey.
authors.forename: Ceren Börçek
authors.initials: CB
authors.lastname: Kasurka
authors.forename: Zülal Atlı
authors.initials: ZA
authors.lastname: Sekeroğlu
authors.forename: Vedat
authors.initials: V
authors.lastname: Sekeroğlu
mesh_terms: D048750:Cell Nucleus Division / Q000187:drug effects; D002478:Cells, Cultured; D002869:Chromosome Aberrations / Q000139:chemically induced; D004305:Dose-Response Relationship, Drug; D005260:Female; D039563:Histamine H1 Antagonists, Non-Sedating / Q000737:chemistry / Q000633:toxicity*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects* / Q000502:physiology; D008297:Male; D015162:Micronucleus Tests; D008940:Mitotic Index; D015394:Molecular Structure; D016593:Terfenadine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000633:toxicity; D018675:Toxicity Tests / Q000379:methods*; D055815:Young Adult
chemical_list: D039563:Histamine H1 Antagonists, Non-Sedating; D016593:Terfenadine; C093230:fexofenadine