title: Effect of tumor necrosis factor-α on neutralization of ventricular fibrillation in rats with acute myocardial infarction.
abstract: The purpose of this study was to explore the effects of tumor necrosis factor-α (TNF-α) on ventricular fibrillation (VF) in rats with acute myocardial infarction (AMI). Rats were randomly classified into AMI group, sham operation group and recombinant human tumor necrosis factor receptor:Fc fusion protein (rhTNFR:Fc) group. Spontaneous and induced VFs were recorded. Monophasic action potentials (MAPs) among different zones of myocardium were recorded at eight time points before and after ligation and MAP duration dispersions (MAPDds) were calculated. Then expression of TNF-α among different myocardial zones was detected. After ligation of the left anterior descending coronary artery, total TNF-α expression in AMI group began to markedly increase at 10 min, reached a climax at 20-30 min, and then gradually decreased. The time-windows of VFs and MAPDds in the border zone performed in a similar way. At the same time-point, the expression of TNF-α in the ischemia zone was greater than that in the border zone, and little in the non-ischemia zone. Although the time windows of TNF-α expression, the MAPDds in the border zone and the occurrence of VFs in the rhTNFR:Fc group were similar to those in the AMI group, they all decreased in the rhTNFR:Fc group. Our findings demonstrate that TNF-α could enlarge the MAPDds in the border zone, and promote the onset of VFs.
authors.affiliation: Department of Cardiology, Wuhan Union Hospital and Institute of Cardiovascular Disease, Tongji Medical College, Huazhong University of Science and Technology, Jiefang Road 1277, Jianghan District, Wuhan 430022, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhang
authors.forename: Yu-Hua
authors.initials: YH
authors.lastname: Liao
authors.forename: Zhe
authors.initials: Z
authors.lastname: Cao
authors.forename: Yi-Mei
authors.initials: YM
authors.lastname: Du
authors.forename: Jia-Ding
authors.initials: JD
authors.lastname: Xia
authors.forename: Hua
authors.initials: H
authors.lastname: Yang
authors.forename: Zhi-Jian
authors.initials: ZJ
authors.lastname: Chen
mesh_terms: D000208:Acute Disease; D000818:Animals; D007150:Immunohistochemistry; D008297:Male; D009203:Myocardial Infarction / Q000235:genetics / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*; D014693:Ventricular Fibrillation / Q000235:genetics / Q000378:metabolism*
chemical_list: D014409:Tumor Necrosis Factor-alpha