title: Down-regulation of c-Myc expression inhibits the invasion of bile duct carcinoma cells.
abstract: Cholangiocarcinoma is the second most common primary hepatic tumour originating from biliary tract epithelial cells with poor prognosis. Enhanced c-Myc protein expression contributes to many aspects of tumour cell biology. Although the ability of c-Myc to drive unrestricted cell proliferation and to inhibit cell differentiation had been well recognized, whether down-regulated c-Myc expression can inhibit tumour cell invasion still remains to be explored. The c-Myc ASODN (antisense oligodeoxyribonucleotide) and NSODN (nonsense oligodeoxyribonucleotide) were designed, synthesized and transfected into human QBC939 bile duct carcinoma cells using the Lipofectamine 2000 reagent. The protein expression of c-Myc was detected by Western blot. A transwell experiment was applied to evaluate the invasive capacity of the QBC939 cells. c-Myc ASODN could significantly suppress the c-Myc protein expression (P<0.05) and the invasion (P<0.01) of QBC939 cells transfected with c-Myc ASODN compared with that in the control and c-Myc NSODN-transfected group. Thus in the present study we show that down-regulation of c-Myc expression can inhibit the invasion of QBC939 cells in vitro.
authors.affiliation: Department of Hepatobiliary Surgery, Hainan Provincial Peoples Hospital, Haikou 570311, Peoples Republic of China. LZR59@hotmail.com
authors.forename: Zhuo-Ri
authors.initials: ZR
authors.lastname: Li
authors.forename: Yi-Fei
authors.initials: YF
authors.lastname: Wu
authors.forename: Chun-Yang
authors.initials: CY
authors.lastname: Ma
authors.forename: Shen-Dan
authors.initials: SD
authors.lastname: Nie
authors.forename: Xian-Hai
authors.initials: XH
authors.lastname: Mao
authors.forename: Yong-Zhong
authors.initials: YZ
authors.lastname: Shi
mesh_terms: D001650:Bile Duct Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D001653:Bile Ducts, Intrahepatic / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D018281:Cholangiocarcinoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D018389:Codon, Nonsense / Q000235:genetics / Q000494:pharmacology; D015536:Down-Regulation / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008099:Liver / Q000473:pathology; D008113:Liver Neoplasms / Q000378:metabolism; D009361:Neoplasm Invasiveness; D020319:Oligodeoxyribonucleotides, Antisense / Q000235:genetics / Q000494:pharmacology; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis* / Q000235:genetics; D014162:Transfection
chemical_list: D018389:Codon, Nonsense; C489427:MYC protein, human; D020319:Oligodeoxyribonucleotides, Antisense; D016271:Proto-Oncogene Proteins c-myc