title: Detection of Dobrava-Belgrade hantavirus using recombinant-nucleocapsid-based enzyme-linked immunosorbent assay and SYBR Green-based real-time reverse transcriptase-polymerase chain reaction.
abstract: Dobrava (DOBV) hantaviruses belong to the genus Hantavirus, family Bunyaviridae, and are carried by yellow-necked and striped field mice. The goal of this study was to detect DOBV using serological and genetic methods in Apodemus rodents in Hungary and in northern Croatia. During the study period, a total of 125 Apodemus sp. (67 A. agrarius, 58 A. flavicollis) were tested for the presence of hantaviruses, and 21 rodents (17%) were positive by rRT-PCR and/or ELISA. We conclude that the prevalence of DOBV is much higher than previously anticipated. The simultaneous use of molecular and serological techniques provides a highly reliable way to detect hantavirus infections.
authors.affiliation: Virological Research Group, Faculty of Sciences, Institute of Biology, University of Pécs, Ifjúság út 6, 7624 Pecs, Hungary.
authors.forename: Viktória
authors.initials: V
authors.lastname: Németh
authors.forename: Mónika
authors.initials: M
authors.lastname: Madai
authors.forename: Alexandra
authors.initials: A
authors.lastname: Maráczi
authors.forename: Bálint
authors.initials: B
authors.lastname: Bérczi
authors.forename: Gyozo
authors.initials: G
authors.lastname: Horváth
authors.forename: Miklós
authors.initials: M
authors.lastname: Oldal
authors.forename: Péter
authors.initials: P
authors.lastname: Kisfali
authors.forename: Krisztián
authors.initials: K
authors.lastname: Bányai
authors.forename: Ferenc
authors.initials: F
authors.lastname: Jakab
mesh_terms: D000818:Animals; D017523:Croatia / Q000453:epidemiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D005456:Fluorescent Dyes; D006476:Orthohantavirus / Q000235:genetics / Q000302:isolation & purification*; D006814:Hungary / Q000453:epidemiology; D008297:Male; D051189:Murinae / Q000821:virology*; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012376:Rodent Diseases / Q000453:epidemiology / Q000821:virology
chemical_list: D005456:Fluorescent Dyes