title: Isolation and characterization of polymorphic nuclear microsatellite loci in Pinus cembra L.
abstract: We developed eight polymorphic nuclear microsatellite markers for the Swiss stone pine (Pinus cembra L.), of which seven may be amplified in a multiplex polymerase chain reaction. Allelic polymorphism across all loci and 40 individuals representing two populations in the Swiss Alps was high (mean = 7.6 alleles). No significant linkage disequlibrium was displayed between pairs of loci. Significant deviation from Hardy-Weinberg equilibrium was revealed at three loci in one population. Cross-amplification was achieved in two related species within the genus (P. sibirica and P. pumila). Thus, the markers may be useful for population genetic studies in these three pine species. They will be applied in ongoing projects on genetic diversity and patterns of gene flow in P. cembra.
authors.affiliation: WSL Swiss Federal Research Institute, Zürcherstrasse 111, 8903 Birmensdorf, Switzerland, Dipartimento di Biotecnologie Agrarie, Genexpress, Università degli Studi di Firenze, Via della Lastruccia 14, 50019 Sesto Fiorentino, Firenze, Italy, Istituto di Genetica Vegetale, Consiglio Nazionale delle Ricerche, Via Madonna del Piano 10, 50019 Sesto Fiorentino, Firenze, Italy.
authors.forename: K
authors.initials: K
authors.lastname: Salzer
authors.forename: F
authors.initials: F
authors.lastname: Sebastiani
authors.forename: F
authors.initials: F
authors.lastname: Gugerli
authors.forename: A
authors.initials: A
authors.lastname: Buonamici
authors.forename: G G
authors.initials: GG
authors.lastname: Vendramin