title: ARHGAP30 is a Wrch-1-interacting protein involved in actin dynamics and cell adhesion.
abstract: The atypical Rho GTPase Wrch-1 has been proposed roles in cell migration, focal adhesion dissolution, stress fibre break down and tight junction heterogeneity. A screen for Wrch-1 binding-partners identified the novel RhoGAP protein, ARHGAP30, as a Wrch-1 interactor. ARHGAP30 is related to the Cdc42- and Rac1-specific RhoGAP CdGAP, which was likewise found to bind Wrch-1. In contrast to CdGAP, ARHGAP30 serves as a Rac1- and RhoA-specific RhoGAP. Ectopic expression of ARHGAP30 results in membrane blebbing and dissolution of stress-fibres and focal adhesions. Our data suggest roles for ARHGAP30 and CdGAP in regulation of cell adhesion downstream of Wrch-1.
authors.affiliation: Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Latifa
authors.initials: L
authors.lastname: Naji
authors.forename: Dirk
authors.initials: D
authors.lastname: Pacholsky
authors.forename: Pontus
authors.initials: P
authors.lastname: Aspenström
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D002448:Cell Adhesion; D002478:Cells, Cultured; D020690:GTPase-Activating Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D051379:Mice; D010802:Phylogeny; D013552:Swine; D020741:rho GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: C559942:ARHGAP30 protein, human; D000199:Actins; D020690:GTPase-Activating Proteins; C432668:RHOU protein, human; D020741:rho GTP-Binding Proteins