title: Strain-specific detection by pulsed-field gel electrophoresis of Lactobacillus gasseri TMC0356 in human feces after oral administration of these organisms.
abstract: The present study aimed to develop an innovative, strain-specific means of identifying the probiotic Lactobacillus gasseri TMC0356 and to determine whether orally administered TMC0356 could be recovered from the human intestine. High molecular weight genomic DNA was isolated from TMC0356 and 14 reference strains of L. gasseri, including the type strain. The DNA samples were digested with the selected rare-cutting restriction endonucleases SmaI, SacII and ApaI and the resulting fragments separated by pulsed-field gel electrophoresis (PFGE) in a size range between 20 to 290 kb. TMC0356 could be distinguished from the other L. gasseri strains on the basis of the SmaI and SacII macrorestriction patterns. Furthermore, L. gasseri strains isolated from the feces of subjects who had ingested TMC0356 were identical to TMC0356 in the SmaI, SacII and ApaI macrorestriction fragments of digested DNA. These results suggest that PFGE of genomic DNA digested with SmaI, SacII, could be a practical means of identification of TMC0356. Furthermore, these results indicate that ingested TMC0356 can survive in, and colonize, the human intestine.
authors.affiliation: Technical Research Laboratory, Takanashi Milk Products, 5 Honjyuku-cho, Asahi-ku, Yokohama, Kanagawa 241-0023, Japan.
authors.forename: Manabu
authors.initials: M
authors.lastname: Kawase
authors.forename: Fang
authors.initials: F
authors.lastname: He
authors.forename: Akira
authors.initials: A
authors.lastname: Kubota
authors.forename: Kenji
authors.initials: K
authors.lastname: Miyazawa
authors.forename: Kazutoyo
authors.initials: K
authors.lastname: Yoda
authors.forename: Masaru
authors.initials: M
authors.lastname: Hiramatsu
mesh_terms: D000284:Administration, Oral; D000328:Adult; D001431:Bacteriological Techniques / Q000379:methods*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D005243:Feces / Q000382:microbiology*; D006801:Humans; D007778:Lactobacillus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D019936:Probiotics / Q000008:administration & dosage*
chemical_list: D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes