title: Optimization of microwave-assisted FeCl3 pretreatment conditions of rice straw and utilization of Trichoderma viride and Bacillus pumilus for production of reducing sugars.
abstract: In this study, Box-Behnken design (BBD) and response surface methodology (RSM) were used to optimize microwave-assisted FeCl(3) pretreatment conditions of rice straw with respect to FeCl(3) concentration, microwave intensity, irradiation time and substrate concentration. When rice straw was pretreated at the optimal conditions of FeCl(3) concentration, 0.14 mol/L; microwave intensity, 160°C; irradiation time, 19 min; substrate concentration, 109 g/L; and inoculated with Trichoderma viride and Bacillus pumilus, the production of reducing sugars was 6.62 g/L. This yield was 2.9 times higher than that obtained with untreated rice straw. The microorganisms degraded 37.8% of pretreated rice straw after 72 h. The structural characteristic analyses suggest that microwave-assisted FeCl(3) pretreatment damaged the silicified waxy surface of rice straw, disrupted almost all the ether linkages between lignin and carbohydrates, and removed lignin.
authors.affiliation: School of Resource and Environmental Science, Hubei Biomass-Resource Chemistry and Environmental Biotechnology Key Laboratory, Wuhan University, Wuhan 430079, China. ljl_82919@163.com
authors.forename: Jiliang
authors.initials: J
authors.lastname: Lü
authors.forename: Peijiang
authors.initials: P
authors.lastname: Zhou
mesh_terms: D001407:Bacillus / Q000187:drug effects / Q000378:metabolism*; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D002241:Carbohydrates / Q000096:biosynthesis*; D002712:Chlorides / Q000494:pharmacology*; D003460:Crystallization; D005290:Ferric Compounds / Q000494:pharmacology*; D008855:Microscopy, Electron, Scanning; D008872:Microwaves; D012275:Oryza / Q000737:chemistry* / Q000187:drug effects; D010084:Oxidation-Reduction / Q000187:drug effects; D015203:Reproducibility of Results; D017550:Spectroscopy, Fourier Transform Infrared; D014242:Trichoderma / Q000378:metabolism*; D014866:Waste Products / Q000032:analysis
chemical_list: D002241:Carbohydrates; D002712:Chlorides; D005290:Ferric Compounds; D014866:Waste Products; C024555:ferric chloride