title: Repair of long bone defects with demineralized bone matrix and autogenous bone composite.
abstract: BACKGROUND
  Repair of diaphyseal bone defects is a challenging problem for orthopedic surgeons. In large bone defects the quantity of harvested autogenous bone may not be sufficient to fill the gap and then the use of synthetic or allogenic grafts along with autogenous bone becomes mandatory to achieve compact filling. Finding the optimal graft mixture for treatment of large diaphyseal defects is an important goal in contemporary orthopedics and this was the main focus of this study. The aim of this study is to investigate the efficacy of demineralized bone matrix (DBM) and autogenous cancellous bone (ACB) graft composite in a rabbit bilateral ulna segmental defect model.
  
  
  METHODS
  Twenty-seven adult female rabbits were divided into five groups. A two-centimeter piece of long bone on the midshaft of the ulna was osteotomized and removed from the rabbits' forearms. In group 1 (n=7) the defects were treated with ACB, in group 2 (n=7) with DBM, and in group 3 (n=7) with ACB and DBM in the ratio of 1:1. Groups 4 and 5, with three rabbits in each group, were the negative and positive controls, respectively. Twelve weeks after implantation the rabbits were sacrificed and union was evaluated with radiograph (Faxitron), dual-energy x-ray absorptiometry (DEXA), and histological methods (decalcified sectioning).
  
  
  RESULTS
  UNION RATES AND THE VOLUME OF NEW BONE IN THE DIFFERENT GROUPS WERE AS FOLLOWS: group 1 - 92.8% union and 78.6% new bone; group 2 - 72.2% union and 63.6% new bone; and group 3 - 100% union and 100% new bone. DEXA results (bone mineral density [BMD]) were as follows: group 1 - 0.164 g/cm(2), group 2 - 0.138 g/cm(2), and group 3 - 0.194 g/cm(2).
  
  
  CONCLUSIONS
  DBM serves as a graft extender or enhancer for autogenous graft and decreases the need of autogenous bone graft in the treatment of bone defects. In this study, the DBM and ACB composite facilitated the healing process. The union rate was better with the combination than with the use of any one of these grafts alone.
authors.affiliation: Department of Orthopedics, Corlu Military Hospital, Corlu, Tekirdag, and.
authors.forename: Mehmet T
authors.initials: MT
authors.lastname: Ozdemir
authors.forename: Mustafa Ç
authors.initials: MÇ
authors.lastname: Kir