title: [Effects of Herba Ephedra Sinicae and Fructus Schisandrae Chinensis on pathology of rats with bleomycin A(5)-induced idiopathic pulmonary fibrosis].
abstract: OBJECTIVE
  To observe the different effects between Mahuang (Herba Ephedra Sinicae) and Wuweizi (Fructus Schisandrae Chinensis) on the pathological changes of rats with bleomycin A(5)-induced pulmonary fibrosis.
  
  
  METHODS
  Ninety Wistar rats were randomly divided into sham operation group, model group, hydrocortisone group, Herba Ephedra Sinicae group, Fructus Schisandrae Chinensis group and Herba Ephedra Sinicae plus Fructus Schisandrae Chinensis group. There were 16 rats in each group except the sham operation group (10 rats). Pulmonary fibrosis was induced by a single intratracheal injection of bleomycin A5. Hematoxylin and eosin straining and immunohistochemical method were used after 7- and 28-day treatment to observe the pathology of lung injury, measure the inner diameter of pulmonary arterioles and the density of nuclear membrane.
  
  
  RESULTS
  Compared with the sham operation group at 7 and 28 d, alveolar inflammation level was significantly increased in the model group (P<0.01). Alveolar inflammation level was decreased obviously in the hydrocortisone group (P<0.05) after 7- and 28-day treatment as compared with the model group, and that in Herba Ephedra Sinicae plus Fructus Schisandrae Chinensis group was also decreased obviously (P<0.05) at 28 d. Compared with the sham operation group, nuclear density of the model group was increased, while its inner diameter was decreased (P<0.05). In the Fructus Schisandrae Chinensis group, Herba Ephedra Sinicae plus Fructus Schisandrae Chinensis group and hydrocortisone group, the nuclear density was decreased (P<0.05) as compared with the model group. Inner diameter in the Herba Ephedra Sinicae group, Herba Ephedra Sinicae plus Fructus Schisandrae Chinensis group and hydrocortisone group was higher than that in the model group (P<0.05). Microvessel density of the model group was obviously higher than that of the sham operation group (P<0.05). Compared with the model group, Herba Ephedra Sinicae plus Fructus Schisandrae Chinensis group and hydrocortisone group had lower microvessel density (P<0.05).
  
  
  CONCLUSIONS
  Herba Ephedra Sinicae combined with Fructus Schisandrae Chinensis can restrain pulmonary artery injury. The nuclear density and microvessel density can be reduced by Fructus Schisandrae Chinensis, while Herba Ephedra Sinicae can increase the inner diameter.
authors.affiliation: Beijing Normal University, Beijing 100875, China.
authors.forename: Hua-qiang
authors.initials: HQ
authors.lastname: Zhai
authors.forename: Shuo-feng
authors.initials: SF
authors.lastname: Zhang
authors.forename: Ming-chao
authors.initials: MC
authors.lastname: Gao
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Min
authors.initials: M
authors.lastname: Ou
authors.forename: Fan-yun
authors.initials: FY
authors.lastname: Meng
authors.forename: Yong-yan
authors.initials: YY
authors.lastname: Wang
mesh_terms: D000818:Animals; D001761:Bleomycin / Q000009:adverse effects / Q000031:analogs & derivatives; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D029790:Ephedra sinica / Q000737:chemistry; D008168:Lung / Q000187:drug effects* / Q000473:pathology*; D008297:Male; D011658:Pulmonary Fibrosis / Q000188:drug therapy / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D032141:Schisandra / Q000737:chemistry
chemical_list: D004365:Drugs, Chinese Herbal; D001761:Bleomycin; C025703:bleomycetin