title: Transgenic tetraploid Isatis indigotica expressing Bt Cry1Ac and Pinellia ternata agglutinin showed enhanced resistance to moths and aphids.
abstract: Co-expression of multiple genes encoding different kinds of insect resistant proteins has been developed to confer a broader spectrum of pest control. Tetraploid Isatis indigotica Fort was transformed with a plasmid, p3300BP, containing Bacillus thuringiensis                         Cry1Ac gene (Bt) and Pinellia ternata agglutinin gene (Pta) and the selectable marker herbicide resistance gene (Bar) driven by the CaMV35S promoter via Agrobacterium tumefaciens-mediated transformation. The integration and expression of introduced genes in regenerated transgenic plants were confirmed by PCR and Western blot assays. Insect bioassay test demonstrated transgenic lines had significant inhibition to diamondback moths (Plutella xylostella L.) and peach potato aphids (Myzus persicae Sulzer) simultaneously. Our study reported here would be a great motivation for field culture of tetraploid I. indigotica, also providing an efficient molecular breeding strategy to provide insect tolerant plants.
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, and Department of Pharmacy, Changzheng Hospital, Shanghai, 200433, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xiao
authors.forename: Kai
authors.initials: K
authors.lastname: Wang
authors.forename: Ruxian
authors.initials: R
authors.lastname: Ding
authors.forename: Hanming
authors.initials: H
authors.lastname: Zhang
authors.forename: Peng
authors.initials: P
authors.lastname: Di
authors.forename: Junfeng
authors.initials: J
authors.lastname: Chen
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Wansheng
authors.initials: W
authors.lastname: Chen
mesh_terms: D000818:Animals; D001042:Aphids; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001681:Biological Assay; D015153:Blotting, Western; D001947:Breeding / Q000379:methods; D017931:DNA Primers / Q000235:genetics; D060467:Disease Resistance / Q000235:genetics*; D004731:Endotoxins / Q000235:genetics* / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics; D006460:Hemolysin Proteins / Q000235:genetics* / Q000378:metabolism; D031219:Isatis / Q000235:genetics* / Q000378:metabolism; D009036:Moths; D010935:Plant Diseases / Q000235:genetics / Q000469:parasitology*; D037121:Plant Lectins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D057891:Tetraploidy; D014170:Transformation, Genetic
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D017931:DNA Primers; D004731:Endotoxins; D006460:Hemolysin Proteins; C047938:Pinellia ternata lectin; D037121:Plant Lectins; C030956:insecticidal crystal protein, Bacillus Thuringiensis