title: P53 expression in stage iii-iv squamous-cell carcinoma of the larynx - an immunohistochemical study related to clinicopathological, flow-cytometric DNA analysis and prognosis.
abstract: A series of 71 patients undergoing radical surgical resection for stage III and IV laryngeal carcinoma (LC) consecutively diagnosed was prospectively studied in order to evaluate the relative weight of p53 expression in predicting clinical outcome, All the patients taking part in this study were followed up for a median of 18 months (range: 6-41 months). Positive staining for p53 protein was detected in 44 of 71 (62%) of these tumors on paraffin-embedded tissue, even in dysplastic areas. Among the clinico-pathological and biological parameters analyzed, only flow-cytometric S-phase (FCM-S) Values of turners showed a significant relationship to p53 immunostaining (p=0.01). With Kaplan-Meier estimation, in multivariate analysis only high FCM-S (>15.1) was independently related to risk of relapse (RR=5.82), while both FCM-S and site (subglottis) were related to risk of death (RR=6.83 and RR=14.3, respectively). These findings indicate that p53 immunoreactivity, though of no utility as a prognostic indicator, probably plays a role in the early stages of LC tumorigenesis.
authors.affiliation: UNIV PALERMO,CTR ONCOBIOL SPERIMENTALE,CATTEDRA ANAT & ISTOL PATOL R,I-90127 PALERMO,ITALY. UNIV PALERMO,CTR INTERDIPARTIMENTALE RIC ONCOL CLIN,IST CLIN & FISIOPATOL CHIRURG,PALERMO,ITALY. UNIV PALERMO,IST CLIN OTORINOLARINGOIATR B,I-90127 PALERMO,ITALY. UNIV PALERMO,CATTEDRA DIAGNOST ISTO & CITPPATOL,I-90127 PALERMO,ITALY. OSSERV EPIDEMIOL REG SICILIANA,I-90145 PALERMO,ITALY.
authors.forename: R
authors.initials: R
authors.lastname: Tomasino
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authors.lastname: Morello
authors.forename: V
authors.initials: V
authors.lastname: Bazan
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authors.lastname: Nagar
authors.forename: V
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authors.lastname: Tralongo
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authors.lastname: Dardanoni
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authors.lastname: Ingria
authors.forename: G
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authors.lastname: Monteleone
authors.forename: S
authors.initials: S
authors.lastname: Restivo
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authors.lastname: Nuara
authors.forename: E
authors.initials: E
authors.lastname: Daniele
authors.forename: A
authors.initials: A
authors.lastname: Russo