title: Simultaneous determination of aflatoxins B₁, B₂, G₁, and G₂ in foods and feed materials.
abstract: A high-performance liquid chromatographic method with on-line post-column photochemical derivatization and fluorimetric detection for the simultaneous separation and quantitative determination of aflatoxin (AF) B(1), B(2), G(1), and G(2) in foodstuffs and feed materials is reported.The chromatographic separation is accomplished by using a C(18) column eluted with an isocratic mobile phase consisting of water, methanol, and acetonitrile. The sample preparation requires a simple extraction of aflatoxins with a mixture of water and methanol, and a purification step by immunoaffinity column clean-up. The total analysis time, including sample preparation and chromatographic separation, does not exceed 40 min with a run time of 10 min. The procedure for the determination of aflatoxins in food samples and cereals for animal consumption has been extensively validated, in agreement with Regulation (EC) No. 882/2004, demonstrating the conformity of the method with provisions of Regulation (EC) No. 401/2006 in terms of sensitivity, linearity, selectivity, and precision.
authors.affiliation: Dipartimento di Scienze Agro-Ambientali, Chimica e Difesa Vegetale and BIOAGROMED, Università degli Studi di Foggia, Foggia, Italy.
authors.forename: Marilena
authors.initials: M
authors.lastname: Muscarella
authors.forename: Marco
authors.initials: M
authors.lastname: Iammarino
authors.forename: Donatella
authors.initials: D
authors.lastname: Nardiello
authors.forename: Sonia Lo
authors.initials: SL
authors.lastname: Magro
authors.forename: Carmen
authors.initials: C
authors.lastname: Palermo
authors.forename: Diego
authors.initials: D
authors.lastname: Centonze
mesh_terms: D016604:Aflatoxin B1 / Q000032:analysis*; D000348:Aflatoxins / Q000032:analysis*; D000821:Animal Feed / Q000382:microbiology; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002523:Edible Grain / Q000737:chemistry / Q000382:microbiology; D005506:Food Contamination / Q000032:analysis*
chemical_list: D000348:Aflatoxins; C027955:aflatoxin G1; C029754:aflatoxin G2; C029753:aflatoxin B2; D016604:Aflatoxin B1