title: Isolation and characterization of 145 polymorphic microsatellite loci for the common frog (Rana temporaria).
abstract: We describe primers and polymerase chain reaction conditions to amplify 145 di-, tri- and tetranucleotide microsatellite loci from the common frog (Rana temporaria), a species commonly used as a model in ecological and evolutionary research. Primers were tested on 46 individuals from two Fennoscandian populations and yielded an average of six to nine alleles per locus (range = 1-30) depending on the population. Average observed heterozygosities in the two populations were 0.16 (range = 0-0.91) and 0.36 (range = 0-1).
authors.affiliation: Ecological Genetics Research Unit, Department of Biological and Environmental Sciences, University of Helsinki, PO Box 65, FI-00014 Helsinki, Finland.
authors.forename: Chikako
authors.initials: C
authors.lastname: Matsuba
authors.forename: Juha
authors.initials: J
authors.lastname: Merilä