title: Dynamic variations in the peripheral blood lymphocyte subgroups of patients with 2009 pandemic H1N1 swine-origin influenza A virus infection.
abstract: BACKGROUND
  Novel Influenza A (H1N1) is an acute respiratory infectious disease. Animal experiments indicated that when H1N1 virus infected early hosts, it showed strong CD4(+), CD8(+), and CD4(+)CD25(+) T cell reactions. The aim of this study was to investigate the dynamic fluctuations of the peripheral blood lymphocyte subgroups in patients infected with H1N1 swine-origin influenza A virus (S-OIV).
  
  
  METHODS
  The frequency of T cells, B cells, natural killer (NK) cells, and regulatory T cells (Treg) in 36 severe H1N1 and 40 moderate H1N1 patients were detected at different periods by flow cytometry. In parallel, serum cytokines were detected by enzyme-linked immunosorbent assay and C-reactive protein (CRP) was analyzed through an image-type automatic biochemical analyzer. In addition, 20 healthy volunteers, who were not infected with 2009 H1N1 virus, were selected as controls.
  
  
  RESULTS
  The frequency of NK cells were decreased in all cases and CD19(+) B cells were increased in severe cases than those of the controls. At 1-2d from onset, the frequency of CD4(+) and CD4(+)CD25(+) T cells in moderate cases was higher than in the severe cases. Serum cytokines, specifically IL-2, IL-4, IL-6, IL-10, and IFN-γ exhibited no significant change both in the moderate and the severe cases during the whole monitoring process. In the early stage of the disease, serum CRP levels in the severe and moderate groups were significantly higher than that in the control group.
  
  
  CONCLUSIONS
  Patients showed different lymphocyte subgroup distributions between mild and severe cases, which might affect the incidence and development of 2009 H1N1.
authors.affiliation: State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310003, PR China.
authors.forename: Xichao
authors.initials: X
authors.lastname: Guo
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
authors.forename: Xuefen
authors.initials: X
authors.lastname: Li
authors.forename: Haishen
authors.initials: H
authors.lastname: Kong
authors.forename: Shigui
authors.initials: S
authors.lastname: Yang
authors.forename: Bo
authors.initials: B
authors.lastname: Ye
authors.forename: Dawei
authors.initials: D
authors.lastname: Cui
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.forename: Lanjuan
authors.initials: L
authors.lastname: Li
mesh_terms: D000328:Adult; D000818:Animals; D001769:Blood / Q000276:immunology*; D002097:C-Reactive Protein / Q000032:analysis; D016207:Cytokines / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology*; D007251:Influenza, Human / Q000276:immunology* / Q000473:pathology / Q000821:virology; D016131:Lymphocyte Subsets / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D013997:Time Factors
chemical_list: D016207:Cytokines; D002097:C-Reactive Protein