title: Development and characterization of polymorphic microsatellite loci in the endangered Miami blue butterfly (Cyclargus thomasi bethunebakeri).
abstract: The Miami blue butterfly (Cyclargus thomasi bethunebakeri) is a state-endangered taxon in Florida and a candidate for federal listing. Here we report 12 polymorphic microsatellite loci appropriate for use in population and conservation studies. We genotyped 114 individuals sampled from a metapopulation in the lower Florida Keys over a 2-year period (2005-2006). These results show 4-14 alleles per locus, and ranges of observed and expected heterozygosities are 0.02679-0.79630 and 0.06154-0.69565, respectively. Large deviations from Hardy-Weinberg equilibrium (HWE) are observed across the whole sample set. When a single breeding population is analysed alone, seven of the loci are in HWE.
authors.affiliation: University of Florida, Department of Entomology and Nematology, PO Box 110620, Gainesville, FL 32611-0620, USA, Florida Museum of Natural History, McGuire Center for Lepidoptera and Biodiversity, Gainesville, FL 32611, USA.
authors.forename: Emily V
authors.initials: EV
authors.lastname: Saarinen
authors.forename: Jaret C
authors.initials: JC
authors.lastname: Daniels
authors.forename: James E
authors.initials: JE
authors.lastname: Maruniak