title: Barley stripe mosaic virus-induced gene silencing (BSMV-IGS) as a tool for functional analysis of barley genes potentially involved in nonhost resistance.
abstract: Barley is an alternative host for the rice blast fungus Magnaporthe oryzae but is resistant to Magnaporthe species associated with the grass genera Pennisetum and Digitaria. The latter cases are examples for nonhost resistance which confers effective and durable protection to plants against a broad spectrum of pathogens. Comparative transcript profiling of host and nonhost interaction revealed an early and pronounced change in gene expression in epidermal tissue of barley infected with a Magnaporthe nonhost isolate. Interestingly, this set of genes did not overlap considerably with the transcriptional response of barley against nonhost rust or powdery mildew isolates. For a functional testing of candidate genes a combined approach of virus-induced gene silencing (VIGS) and subsequent pathogen challenge was established. As anticipated, VIGS-mediated down-regulation of Mlo-transcripts led to higher resistance against Blumeria graminis f.sp. hordei and enhanced susceptibility against M. oryzae.
authors.affiliation: Department of Plant Physiology, RWTH Aachen University, Aachen, Germany.
authors.forename: Rhoda
authors.initials: R
authors.lastname: Delventhal
authors.forename: Nina
authors.initials: N
authors.lastname: Zellerhoff
authors.forename: Ulrich
authors.initials: U
authors.lastname: Schaffrath
mesh_terms: D060467:Disease Resistance / Q000235:genetics*; D020868:Gene Silencing; D017343:Genes, Plant / Q000235:genetics*; D001467:Hordeum / Q000235:genetics* / Q000276:immunology* / Q000382:microbiology; D009029:Mosaic Viruses / Q000502:physiology*; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology* / Q000382:microbiology