title: Preparation of suspensions of human cervical epithelial-cells suitable for biochemical, cytochemical and immunocytochemical investigations.
abstract: A simple gradient centrifuging method that separates epithelial cells from suspensions of material obtained by conventional cervical sampling procedures, is described. Heterogeneous cell suspensions were disaggregated by syringing and placed on a discontinuous density gradient. Following centrifugation up to 5 bands of, cells could be collected. The three central bands of epithelial cells corresponded to a density range between 1.035g/ml and 1.055g/ml. The cell samples so obtained are suitable for biochemical, cytochemical and immunocytochemical investigations.
authors.affiliation: BRUNEL UNIV,DEPT BIOL & BIOCHEM,UXBRIDGE UB8 3PH,MIDDX,ENGLAND. QUEENS MED CTR,NOTTINGHAM NG7 2UH,ENGLAND.
authors.forename: T
authors.initials: T
authors.lastname: Slater
authors.forename: J
authors.initials: J
authors.lastname: Beesley
authors.forename: A
authors.initials: A
authors.lastname: Flatman
authors.forename: R
authors.initials: R
authors.lastname: Hammond
authors.forename: S
authors.initials: S
authors.lastname: Jonas