title: On-line monitoring of oxygen in Tubespin, a novel, small-scale disposable bioreactor.
abstract: A novel, optical sensor was fixed in a new type of disposable bioreactor, Tubespin, for the on-line (real-time) monitoring of dissolved oxygen concentrations during cell culture. The cell density, viability and volumetric mass transfer coefficient were also determined to further characterize the bioreactors. The k(L)a value of the Tubespin at standard conditions was 24.3 h(-1), while that of a spinner flask was only 2.7 h(-1). The maximum cell density in the Tubespin bioreactor reached 6 × 10(6) cells mL(-1), which was two times higher than the cell density in a spinner flask. Furthermore, the dynamic dissolved oxygen level was maintained above 90% air-saturation in the Tubespin, while the value was only 1.9% in a spinner flask. These results demonstrate the competitive advantage of using the Tubespin system over spinner flasks for process optimization and scale-down studies of oxygen transfer and cell growth.
authors.affiliation: Biomedical R&D Center, Guangdong Provincial Key Laboratory of Bioengineering Medicine, National Engineering Research Center of Genetic Medicine, Jinan University, 5/F Building of Biology, Guangzhou, 510632, Guangdong, People's Republic of China.
authors.forename: Kui
authors.initials: K
authors.lastname: Xie
authors.forename: Xiao-Wei
authors.initials: XW
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Huang
authors.forename: Yi-Ting
authors.initials: YT
authors.lastname: Wang
authors.forename: Yun
authors.initials: Y
authors.lastname: Lei
authors.forename: Jing
authors.initials: J
authors.lastname: Rong
authors.forename: Chui-Wen
authors.initials: CW
authors.lastname: Qian
authors.forename: Qiu-Ling
authors.initials: QL
authors.lastname: Xie
authors.forename: Yi-Fei
authors.initials: YF
authors.lastname: Wang
authors.forename: An
authors.initials: A
authors.lastname: Hong
authors.forename: Sheng
authors.initials: S
authors.lastname: Xiong