title: Hepatitis B virus induces G1 phase arrest by regulating cell cycle genes in HepG2.2.15 cells.
abstract: BACKGROUND
  To investigate the effect of HBV on the proliferative ability of host cells and explore the potential mechanism.
  
  
  METHODS
  MTT, colony formation assay and tumourigenicity in nude mice were performed to investigate the effect of HBV on the proliferative capability of host cells. In order to explore the potential mechanism, cell cycle and apoptosis were analysed. The cell cycle genes controlling the G1/S phase transition were detected by immunohistochemistry, westernblot and RT-PCR.
  
  
  RESULTS
  HepG2.2.15 cells showed decreased proliferation ability compared to HepG2 cells. G1 phase arrest was the main cause but was not associated with apoptosis. p53, p21 and total retinoblastoma (Rb) were determined to be up-regulated, whereas cyclinE was down-regulated at both the protein and mRNA levels in HepG2.2.15 cells. The phosphorylated Rb in HepG2.2.15 cells was decreased.
  
  
  CONCLUSIONS
  Our results suggested that HBV inhibited the capability of proliferation of HepG2.2.15 cells by regulating cell cycle genes expression and inducing G1 arrest.
authors.affiliation: Department of Pathology, Basic Medical Science College, Harbin Medical University, 157 Baojian Road, Nangang District, Harbin 150081, China.
authors.forename: Tianzhen
authors.initials: T
authors.lastname: Wang
authors.forename: Ran
authors.initials: R
authors.lastname: Zhao
authors.forename: Yiqi
authors.initials: Y
authors.lastname: Wu
authors.forename: Dan
authors.initials: D
authors.lastname: Kong
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Di
authors.initials: D
authors.lastname: Wu
authors.forename: Chao
authors.initials: C
authors.lastname: Li
authors.forename: Chong
authors.initials: C
authors.lastname: Zhang
authors.forename: Zuxi
authors.initials: Z
authors.lastname: Yu
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Jin
mesh_terms: D000818:Animals; D018797:Cell Cycle Proteins / Q000037:antagonists & inhibitors*; D002460:Cell Line; D002470:Cell Survival; D005260:Female; D016193:G1 Phase; D006515:Hepatitis B virus / Q000472:pathogenicity*; D022781:Hepatocytes / Q000821:virology*; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009369:Neoplasms / Q000517:prevention & control; D013194:Staining and Labeling / Q000379:methods; D013234:Stem Cells; D013778:Tetrazolium Salts / Q000378:metabolism; D013844:Thiazoles / Q000378:metabolism
chemical_list: D018797:Cell Cycle Proteins; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue