title: Water extracts of Brazilian leguminous seeds as rich sources of larvicidal compounds against Aedes aegypti L.
abstract: This study assessed the toxicity of seed water extracts of 15 leguminous species upon Aedes aegypti larvae. A partial chemical and biochemical characterization of water extracts, as well as the assessment of their acute toxicity in mice, were performed. The extracts of Amburana cearensis, Anadenanthera macrocarpa, Dioclea megacarpa, Enterolobium contortisiliquum and Piptadenia moniliformis caused 100% of mortalit y after 1 to 3 h of exposure. They showed LC(50) and LC(90) values ranging from 0.43 ± 0.01 to 9.06 ± 0.12 mg/mL and from 0.71 ± 0.02 to 13.03 ± 0.15 mg/mL, respectively. Among the secondary metabolite constituents, the seed water extracts showed tannins, phenols, flavones, favonols, xanthones, saponins and alkaloids. The extracts also showed high soluble proteins content (0.98 to 7.71 mg/mL), lectin (32 to 256 HU/mL) and trypsin inhibitory activity (3.64 = 0.43 to 26.19 = 0.05 gIT/kg of flour) The electrophoretic profiles showed a great diversity of protein bands, many of which already described as insecticide proteins. The extracts showed low toxicity to mice (LD(50) > 0.15 = 0.01 g/kg body weight), but despite these promising results, further studies are necessary to understand the toxicity of these extracts and their constituents from primary and secondary metabolism upon Ae. aegypti.
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Campus do Pici Universidade Federal do Ceará, Fortaleza, CE, Brazil.
authors.forename: Davi F
authors.initials: DF
authors.lastname: Farias
authors.forename: Mariana G
authors.initials: MG
authors.lastname: Cavalheiro
authors.forename: Martônio P
authors.initials: MP
authors.lastname: Viana
authors.forename: Vanessa A
authors.initials: VA
authors.lastname: Queiroz
authors.forename: Lady C B
authors.initials: LC
authors.lastname: Rocha-Bezerra
authors.forename: Ilka M
authors.initials: IM
authors.lastname: Vasconcelos
authors.forename: Selene M
authors.initials: SM
authors.lastname: Morais
authors.forename: Ana F U
authors.initials: AF
authors.lastname: Carvalho
mesh_terms: D000330:Aedes / Q000187:drug effects*; D000818:Animals; D007887:Fabaceae / Q000737:chemistry / Q000145:classification; D007306:Insecticides; D007814:Larva / Q000187:drug effects; D051379:Mice; D010936:Plant Extracts; D012639:Seeds / Q000737:chemistry; D023382:Toxicity Tests, Acute
chemical_list: D007306:Insecticides; D010936:Plant Extracts