title: Enhancing Jatropha oil extraction yield from the kernels assisted by a xylan-degrading bacterium to preserve protein structure.
abstract: We investigated the use of bacterial cells isolated from paddy crab for the extraction of oil from Jatropha seed kernels in aqueous media while simultaneously preserving the protein structures of this protein-rich endosperm. A bacterial strain-which was marked as MB4 and identified by means of 16S rDNA sequencing and physiological characterization as either Bacillus pumilus or Bacillus altitudinis-enhanced the extraction yield of Jatropha oil. The incubation of an MB4 starter culture with preheated kernel slurry in aqueous media with the initial pH of 5.5 at 37 °C for 6 h liberated 73% w/w of the Jatropha oil. Since MB4 produces xylanases, it is suggested that strain MB4 facilitates oil liberation via degradation of hemicelluloses which form the oil-containing cell wall structure of the kernel. After MB4 assisted oil extraction, SDS-PAGE analysis showed that the majority of Jatropha proteins were preserved in the solid phase of the extraction residues. The advantages offered by this process are: protein in the residue can be further processed for other applications, no purified enzyme preparation is needed, and the resulting oil can be used for biodiesel production.
authors.affiliation: Valorisation of Plant Production Chains, Wageningen University, Wageningen, the Netherlands. ahmad.marasabessy@wur.nl
authors.forename: Ahmad
authors.initials: A
authors.lastname: Marasabessy
authors.forename: Maelita R
authors.initials: MR
authors.lastname: Moeis
authors.forename: Johan P M
authors.initials: JP
authors.lastname: Sanders
authors.forename: Ruud A
authors.initials: RA
authors.lastname: Weusthuis
mesh_terms: D000818:Animals; D001407:Bacillus / Q000201:enzymology* / Q000302:isolation & purification / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods*; D003386:Brachyura / Q000382:microbiology; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D031286:Jatropha / Q000737:chemistry*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010938:Plant Oils / Q000302:isolation & purification*; D011506:Proteins / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013696:Temperature; D013997:Time Factors; D014990:Xylans / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D010938:Plant Oils; D011506:Proteins; D012336:RNA, Ribosomal, 16S; D014990:Xylans