title: Isolation and characterization of 11 microsatellite loci from the black fly, Simulium negativum (Diptera: Simuliidae).
abstract: Eleven polymorphic microsatellite loci were isolated and developed from the black fly, Simulium negativum, a member of the Simulium arcticum sibling species complex. The observed heterozygosity of the 11 loci ranged from 0.03 to 0.83. The number of alleles per locus ranged from 8 to 19. Significant linkage disequilibria were encountered only for the primer pairs BF7-1 with BF7-5 and BF6-32 with BF7-16. Presumably, these microsatellite loci can be used to study genetic structure within the entire S. arcticum complex.
authors.affiliation: Department of Natural History, Royal Ontario Museum, 100 Queen's Park, Toronto, Ontario, Canada M5S 2C6, Department of Ecology & Evolution Biology, University of Toronto, 25 Willcocks Street, Toronto, Ontario, Canada M5S 3B2.
authors.forename: Mike
authors.initials: M
authors.lastname: Spironello
authors.forename: Leena
authors.initials: L
authors.lastname: Rizvi
authors.forename: Douglas C
authors.initials: DC
authors.lastname: Currie
authors.forename: Christina M
authors.initials: CM
authors.lastname: Davy
authors.forename: Robert W
authors.initials: RW
authors.lastname: Murphy