title: Molecular changes induced by bisphenol-A in rat Sertoli cell culture.
abstract: Bisphenol-A (BPA) is an industrial chemical and is known to act as an endocrine disrupter. This study was designed to evaluate how BPA regulates Sertoli cell (SC) signal molecules. Purified rat SCs were cultured and treated with BPA (200 µmol/l) at various time points. Western blot analysis was used to determine the activation of extracellular signal-related kinases 1 and 2 (ERK1/2), c-Jun N-terminal kinase (JNK), p38 mitogen activated protein kinase (MAPK), nuclear factor kappa B (NF-κB), cyclooxygenase-1,2 (COX-1, 2), estrogen receptor-α (ER-α), and androgen receptor (AR). The levels of transferrin (TF), prostaglandin E(2) (PGE(2)), and prostaglandin F(2α) (PGF(2α)) in culture medium were quantified by ELISA. Interleukin (IL)-1β and IL-6 mRNAs were measured by quantitative real-time PCR (QRT-PCR). Compared with the control, BPA activated the phosphorylation of ERK1/2 (p-ERK1/2) through 30 min to 6 h. TF was down-regulated at 6 and 24 h. Furthermore, IL-1β was up-regulated at 30 min and IL-6 was up-regulated at 1 and 24 h. ERK activity inhibitor (PD98059, 10 µmol/l) inhibited these molecular changes. These results reveal the possibility that BPA may have adverse effects on spermatogenesis via ERK1/2.
authors.affiliation: Division of Urology, Department of Organs Therapeutics, Faculty of Medicine, Kobe University Graduate School of Medicine, Kobe, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Izumi
authors.forename: Kohei
authors.initials: K
authors.lastname: Yamaguchi
authors.forename: Tomomoto
authors.initials: T
authors.lastname: Ishikawa
authors.forename: Makoto
authors.initials: M
authors.lastname: Ando
authors.forename: Koji
authors.initials: K
authors.lastname: Chiba
authors.forename: Hiromi
authors.initials: H
authors.lastname: Hashimoto
authors.forename: Masahide
authors.initials: M
authors.lastname: Shiotani
authors.forename: Masato
authors.initials: M
authors.lastname: Fujisawa
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds; D015153:Blotting, Western; D003470:Culture Media; D015237:Dinoprost / Q000378:metabolism; D015232:Dinoprostone / Q000378:metabolism; D004789:Enzyme Activation; D004797:Enzyme-Linked Immunosorbent Assay; D047628:Estrogen Receptor alpha / Q000378:metabolism; D053583:Interleukin-1beta / Q000235:genetics; D015850:Interleukin-6 / Q000235:genetics; D008297:Male; D016328:NF-kappa B / Q000378:metabolism; D010636:Phenols / Q000494:pharmacology*; D011494:Protein Kinases / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D060888:Real-Time Polymerase Chain Reaction; D011944:Receptors, Androgen / Q000378:metabolism; D012708:Sertoli Cells / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D014168:Transferrin / Q000378:metabolism; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D003470:Culture Media; D015237:Dinoprost; D015232:Dinoprostone; D047628:Estrogen Receptor alpha; D053583:Interleukin-1beta; D015850:Interleukin-6; D016328:NF-kappa B; D010636:Phenols; D011494:Protein Kinases; D012333:RNA, Messenger; D011944:Receptors, Androgen; D014168:Transferrin; C006780:bisphenol A; D000098826:Bisphenol A Compounds