title: Dramatically improved catalytic activity of an artificial (S)-selective arylmalonate decarboxylase by structure-guided directed evolution.
abstract: Using three rounds of structure-guided directed evolution, the catalytic activity of the (S)-selective arylmalonate decarboxylase variant G74C/C188S could be increased up to 920-fold. The best variant had a 220-fold improved activity in the production of (S)-naproxen with excellent enantioselectivity (>99% ee).
authors.affiliation: Department of Biosciences and Informatics, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama, Kanagawa, 223-8522, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Miyauchi
authors.forename: Robert
authors.initials: R
authors.lastname: Kourist
authors.forename: Daisuke
authors.initials: D
authors.lastname: Uemura
authors.forename: Kenji
authors.initials: K
authors.lastname: Miyamoto
mesh_terms: D055162:Biocatalysis; D016950:Bordetella bronchiseptica / Q000201:enzymology; D002262:Carboxy-Lyases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D020134:Catalytic Domain; D019020:Directed Molecular Evolution / Q000379:methods*; D008958:Models, Molecular; D009154:Mutation; D013379:Substrate Specificity; D013455:Sulfur / Q000378:metabolism*
chemical_list: D013455:Sulfur; D002262:Carboxy-Lyases; C071852:malonate decarboxylase