title: A universal code for RNA recognition by PUF proteins.
abstract: The design of proteins that can bind any RNA sequence of interest has many potential biological and medical applications. Here we have expanded the recognition of Pumilio and FBF homology protein (PUF) repeats beyond adenine, guanine and uracil and evolved them to specifically bind cytosine. These repeat sequences can be used to create PUF domains capable of selectively binding RNA targets of diverse sequence and structure.
authors.affiliation: Western Australian Institute for Medical Research and Centre for Medical Research, The University of Western Australia, Perth, Australia.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Filipovska
authors.forename: Muhammad F M
authors.initials: MF
authors.lastname: Razif
authors.forename: Karoline K A
authors.initials: KK
authors.lastname: Nygård
authors.forename: Oliver
authors.initials: O
authors.lastname: Rackham
mesh_terms: D003596:Cytosine / Q000378:metabolism*; D024202:Electrophoretic Mobility Shift Assay; D006801:Humans; D009154:Mutation; D015202:Protein Engineering; D012313:RNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020449:Repetitive Sequences, Amino Acid; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D020798:Two-Hybrid System Techniques
chemical_list: C471242:PUM1 protein, human; D016601:RNA-Binding Proteins; D012313:RNA; D003596:Cytosine