title: Morphological and functional differences in coculture system of keratinocytes and dorsal-root-ganglion-derived cells depending on time of seeding.
abstract: Previous study indicated that in a coculture system of keratinocytes and dorsal-root-ganglion-derived (DRG) cells, mechanical stimulation of keratinocytes induced ATP-mediated calcium propagation and excitation of DRG cells. Here, we examined two different coculture systems of keratinocytes and DRG cells. In one, we seeded keratinocytes first and then seeded DRG cells on the keratinocytes. In this system, nerve fibres from DRG cells passed between keratinocytes. Mechanical stimulation of keratinocytes did not induce excitation of DRG cells. In the other, we seeded both cell types together. At first, each cell type grew separately, forming cell aggregates. Then, nerve fibres grew out from the DRG cell aggregates to keratinocyte aggregates and penetrated into them. In this system, mechanical stimulation of keratinocytes induced excitation of the nerve fibres, but the excitation was not completely blocked by apyrase, an ATP-degrading enzyme. These results suggest that coculture of keratinocytes and DRG can generate a variety of structures, depending on the seeding conditions.
authors.affiliation: Shiseido Research Center, Kanazawa-ku, Yokohama, Japan.
authors.forename: Moe
authors.initials: M
authors.lastname: Tsutsumi
authors.forename: Makiko
authors.initials: M
authors.lastname: Goto
authors.forename: Sumiko
authors.initials: S
authors.lastname: Denda
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Denda
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001081:Apyrase / Q000378:metabolism; D020013:Calcium Signaling; D018920:Coculture Techniques / Q000379:methods*; D005727:Ganglia, Spinal / Q000166:cytology* / Q000502:physiology*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology* / Q000502:physiology*; D009412:Nerve Fibers / Q000502:physiology / Q000648:ultrastructure; D051381:Rats; D013314:Stress, Mechanical; D013997:Time Factors
chemical_list: D000255:Adenosine Triphosphate; D001081:Apyrase