title: Induced migration of dental pulp stem cells for in vivo pulp regeneration.
abstract: Dental pulp has intrinsic capacity for self-repair. However, it is not clear whether dental pulp cells can be recruited endogenously for regenerating pulp tissues, including mineralizing into dentin. This work is based on a hypothesis that dental pulp stem/progenitor cells can be induced to migrate by chemotactic cytokines and act as endogenous cell sources for regeneration and mineralization. Dental stem cells (DSCs) were isolated from adult human tooth pulp and seeded on the surfaces of 3D collagen gel cylinders that were incubated in chemically defined media with stromal-derived factor-1α (SDF1), basic fibroblast growth factor (bFGF), or bone morphogenetic protein-7 (BMP7). Significantly more cells were recruited into collagen gel by SDF1 or bFGF than without cytokines in 7 days, whereas BMP7 had little effect on cell recruitment. BMP7, however, was highly effective, equally to dexamethasone, in orchestrating mineralization of cultured DSCs. Cell membrane receptors for SDF1, bFGF, and BMP7 were up-regulated in treated DSCs. Upon in vivo delivery, bFGF induced re-cellularization and re-vascularization in endodontically treated human teeth implanted into the dorsum of rats. Thus, endogenous dental pulp cells, including stem/progenitor cells, may be recruited and subsequently differentiated by chemotaxis of selective cytokines in the regeneration of dental pulp.
authors.affiliation: Center for Craniofacial Regeneration (CCR), Fu Foundation School of Engineering and Applied Science, Columbia University Medical Center, Columbia University, 630 W. 168 St. – PH7E-CDM, New York, NY 10032, USA.
authors.forename: T
authors.initials: T
authors.lastname: Suzuki
authors.forename: C H
authors.initials: CH
authors.lastname: Lee
authors.forename: M
authors.initials: M
authors.lastname: Chen
authors.forename: W
authors.initials: W
authors.lastname: Zhao
authors.forename: S Y
authors.initials: SY
authors.lastname: Fu
authors.forename: J J
authors.initials: JJ
authors.lastname: Qi
authors.forename: G
authors.initials: G
authors.lastname: Chotkowski
authors.forename: S B
authors.initials: SB
authors.lastname: Eisig
authors.forename: A
authors.initials: A
authors.lastname: Wong
authors.forename: J J
authors.initials: JJ
authors.lastname: Mao
mesh_terms: D000293:Adolescent; D000328:Adult; D053687:Adult Stem Cells / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D000704:Analysis of Variance; D000818:Animals; D055419:Bone Morphogenetic Protein 7 / Q000494:pharmacology; D052004:Bone Morphogenetic Protein Receptors / Q000096:biosynthesis; D002113:Calcification, Physiologic; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D054377:Chemokine CXCL12 / Q000494:pharmacology*; D002633:Chemotaxis / Q000187:drug effects*; D003094:Collagen; D003782:Dental Pulp / Q000166:cytology*; D003790:Dental Pulp Necrosis / Q000188:drug therapy; D023381:Endpoint Determination; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D006801:Humans; D008297:Male; D018919:Neovascularization, Physiologic; D051381:Rats; D019718:Receptors, CXCR4 / Q000096:biosynthesis; D017468:Receptors, Fibroblast Growth Factor / Q000096:biosynthesis; D012038:Regeneration / Q000187:drug effects*; D033581:Stem Cell Transplantation; D040521:Subcutaneous Tissue; D054457:Tissue Scaffolds; D019553:Tooth, Nonvital / Q000188:drug therapy
chemical_list: D055419:Bone Morphogenetic Protein 7; D054377:Chemokine CXCL12; D019718:Receptors, CXCR4; D017468:Receptors, Fibroblast Growth Factor; D016222:Fibroblast Growth Factor 2; D003094:Collagen; D052004:Bone Morphogenetic Protein Receptors