title: Pardaxin-induced apoptosis enhances antitumor activity in HeLa cells.
abstract: Pardaxin, a pore-forming antimicrobial peptide that encodes 33 amino acids was isolated from the Red Sea Moses sole, Pardachirus mamoratus. In this study, we investigated its antitumor activity in human fibrosarcoma (HT-1080) cells and epithelial carcinoma (HeLa) cells. In vitro results showed that the synthetic pardaxin peptide had antitumor activity in these two types of cancer cells and that 15μg/ml pardaxin did not lyse human red blood cells. Moreover, this synthetic pardaxin inhibited the proliferation of HT1080 cells in a dose-dependent manner and induced programmed cell death in HeLa cells. DNA fragmentation and increases in the subG1 phase and caspase 8 activities suggest that pardaxin caused HeLa cell death by inducing apoptosis, but had a different mechanism in HT1080 cells.
authors.affiliation: Graduate Institute of Biotechnology, National Chung-Hsing University, 250 Kuo-Kuang Rd., Taichung 402, Taiwan.
authors.forename: Jung-Chieh
authors.initials: JC
authors.lastname: Hsu
authors.forename: Li-Ching
authors.initials: LC
authors.lastname: Lin
authors.forename: Jason T C
authors.initials: JT
authors.lastname: Tzen
authors.forename: Jyh-Yih
authors.initials: JY
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002277:Carcinoma / Q000188:drug therapy / Q000473:pathology; D053181:Caspase 8 / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D057846:Cell-Penetrating Peptides / Q000138:chemical synthesis / Q000494:pharmacology*; D053938:DNA Fragmentation / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects; D005260:Female; D005354:Fibrosarcoma / Q000188:drug therapy / Q000473:pathology; D029941:Fish Proteins / Q000138:chemical synthesis / Q000494:pharmacology*; D005397:Fish Venoms / Q000138:chemical synthesis / Q000494:pharmacology*; D005400:Fishes, Poisonous / Q000378:metabolism; D016193:G1 Phase / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D009498:Neurotoxins / Q000138:chemical synthesis / Q000494:pharmacology*; D009928:Organ Specificity; D015854:Up-Regulation
chemical_list: D057846:Cell-Penetrating Peptides; D029941:Fish Proteins; D005397:Fish Venoms; D009498:Neurotoxins; C017603:pardaxin; D053181:Caspase 8