title: Separation and identification of norcantharidin metabolites in vivo by GC-MS method.
abstract: Norcantharidin (NCTD), the demethylated analogue of cantharidin, inhibits the proliferation of a variety of human tumor cell lines, and appears to cause the least nephrotoxic and inflammatory side effects. Although NCTD has been used to treat human cancers in China for years, there is no report regarding its metabolism up to now. This is the first report to separate and identify the main metabolites of NCTD in vivo by GC-MS using TMS derivatives. Two hydrolyzed products and five phase I or phase II metabolites were found in rat by the chromatogram comparisons of the blank with incurred biological samples. Multiple stages of fragmentation patterns were used to confirm the metabolites characterizations. The established GC-MS method can also be applied to identifying unknown metabolites of the drugs containing hydroxyl or carbonyl groups in molecular structure.
authors.affiliation: Institute of Clinical Pharmacology, Qilu Hospital of Shandong University, 107#, Wenhua Xi Road, Jinan, Shandong 250012, PR China.
authors.forename: Chunmin
authors.initials: C
authors.lastname: Wei
authors.forename: Yanni
authors.initials: Y
authors.lastname: Teng
authors.forename: Benjie
authors.initials: B
authors.lastname: Wang
authors.forename: Xiumei
authors.initials: X
authors.lastname: Zhang
authors.forename: Guiyan
authors.initials: G
authors.lastname: Yuan
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Liu
authors.forename: Rong
authors.initials: R
authors.lastname: Li
authors.forename: Ruichen
authors.initials: R
authors.lastname: Guo
mesh_terms: D000818:Animals; D001646:Bile / Q000737:chemistry; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000032:analysis* / Q000097:blood / Q000378:metabolism / Q000493:pharmacokinetics; D004365:Drugs, Chinese Herbal; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D053858:Metabolic Networks and Pathways; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D019086:Bridged Bicyclo Compounds, Heterocyclic; D004365:Drugs, Chinese Herbal; C069741:norcantharidin