title: Synthesis, crystal structure and living cell imaging of a Cu(2+)-specific molecular probe.
abstract: We report the development of a rhodamine chromene-based fluorescence probe to monitor the intracellular Cu(2+) level in living cells. The new fluorescent probe exhibits a fluorescence response towards Cu(2+) under physiological conditions with high sensitivity and selectivity, and facilitates the naked-eye detection of Cu(2+). The fluorescence intensity was significantly increased by about 40-fold with 10 equiv. of added Cu(2+).
authors.affiliation: Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan, 250100, P. R. China.
authors.forename: Wei-Yong
authors.initials: WY
authors.lastname: Liu
authors.forename: Hai-Ying
authors.initials: HY
authors.lastname: Li
authors.forename: Bao-Xiang
authors.initials: BX
authors.lastname: Zhao
authors.forename: Jun-Ying
authors.initials: JY
authors.lastname: Miao
mesh_terms: D002413:Cations, Divalent / Q000737:chemistry; D002470:Cell Survival; D003300:Copper / Q000032:analysis* / Q000737:chemistry; D018360:Crystallography, X-Ray; D005456:Fluorescent Dyes / Q000032:analysis* / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D008958:Models, Molecular; D015394:Molecular Structure; D013141:Spiro Compounds / Q000032:analysis* / Q000737:chemistry
chemical_list: D002413:Cations, Divalent; D005456:Fluorescent Dyes; D013141:Spiro Compounds; D003300:Copper