title: Transcriptome analysis reveals salt-stress-regulated biological processes and key pathways in roots of cotton (Gossypium hirsutum L.).
abstract: High salinity is one of the main factors limiting cotton growth and productivity. The genes that regulate salt stress in TM-1 upland cotton were monitored using microarray and real-time PCR (RT-PCR) with samples taken from roots. Microarray analysis showed that 1503 probe sets were up-regulated and 1490 probe sets were down-regulated in plants exposed for 3h to 100mM NaCl, and RT-PCR analysis validated 42 relevant/related genes. The distribution of enriched gene ontology terms showed such important processes as the response to water stress and pathways of hormone metabolism and signal transduction were induced by the NaCl treatment. Some key regulatory gene families involved in abiotic and biotic sources of stress such as WRKY, ERF, and JAZ were differentially expressed. Our transcriptome analysis might provide some useful insights into salt-mediated signal transduction pathways in cotton and offer a number of candidate genes as potential markers of tolerance to salt stress.
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing, PR China.
authors.forename: Dongxia
authors.initials: D
authors.lastname: Yao
authors.forename: Xueyan
authors.initials: X
authors.lastname: Zhang
authors.forename: Xinhua
authors.initials: X
authors.lastname: Zhao
authors.forename: Chuanliang
authors.initials: C
authors.lastname: Liu
authors.forename: Chunchao
authors.initials: C
authors.lastname: Wang
authors.forename: Zhenghai
authors.initials: Z
authors.lastname: Zhang
authors.forename: Chaojun
authors.initials: C
authors.lastname: Zhang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wei
authors.forename: Qianhua
authors.initials: Q
authors.lastname: Wang
authors.forename: Hong
authors.initials: H
authors.lastname: Yan
authors.forename: Fuguang
authors.initials: F
authors.lastname: Li
authors.forename: Zhen
authors.initials: Z
authors.lastname: Su
mesh_terms: D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D005819:Genetic Markers; D003368:Gossypium / Q000187:drug effects / Q000235:genetics*; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics*; D012965:Sodium Chloride / Q000494:pharmacology*; D013312:Stress, Physiological / Q000187:drug effects*; D014157:Transcription Factors / Q000235:genetics
chemical_list: D005819:Genetic Markers; D014157:Transcription Factors; D012965:Sodium Chloride