title: Gene expression profiles in BCL11B-siRNA treated malignant T cells.
abstract: BACKGROUND
  Downregulation of the B-cell chronic lymphocytic leukemia (CLL)/lymphoma11B (BCL11B) gene by small interfering RNA (siRNA) leads to growth inhibition and apoptosis of the human T-cell acute lymphoblastic leukemia (T-ALL) cell line Molt-4. To further characterize the molecular mechanism, a global gene expression profile of BCL11B-siRNA -treated Molt-4 cells was established. The expression profiles of several genes were further validated in the BCL11B-siRNA -treated Molt-4 cells and primary T-ALL cells.
  
  
  RESULTS
  142 genes were found to be upregulated and 109 genes downregulated in the BCL11B-siRNA -treated Molt-4 cells by microarray analysis. Among apoptosis-related genes, three pro-apoptotic genes, TNFSF10, BIK, BNIP3, were upregulated and one anti-apoptotic gene, BCL2L1 was downregulated. Moreover, the expression of SPP1 and CREBBP genes involved in the transforming growth factor (TGF-β) pathway was down 16-fold. Expression levels of TNFSF10, BCL2L1, SPP1, and CREBBP were also examined by real-time PCR. A similar expression pattern of TNFSF10, BCL2L1, and SPP1 was identified. However, CREBBP was not downregulated in the BLC11B-siRNA -treated Molt-4 cells.
  
  
  CONCLUSIONS
  BCL11B-siRNA treatment altered expression profiles of TNFSF10, BCL2L1, and SPP1 in both Molt-4 T cell line and primary T-ALL cells.
authors.affiliation: Institute of Hematology, Medical College, Jinan University, Guangzhou, PR China. yangqiuli@hotmail.com
authors.forename: Xin
authors.initials: X
authors.lastname: Huang
authors.forename: Qi
authors.initials: Q
authors.lastname: Shen
authors.forename: Si
authors.initials: S
authors.lastname: Chen
authors.forename: Shaohua
authors.initials: S
authors.lastname: Chen
authors.forename: Lijian
authors.initials: L
authors.lastname: Yang
authors.forename: Jianyu
authors.initials: J
authors.lastname: Weng
authors.forename: Xin
authors.initials: X
authors.lastname: Du
authors.forename: Piotr
authors.initials: P
authors.lastname: Grabarczyk
authors.forename: Grzegorz K
authors.initials: GK
authors.lastname: Przybylski
authors.forename: Christian A
authors.initials: CA
authors.lastname: Schmidt
authors.forename: Yangqiu
authors.initials: Y
authors.lastname: Li
mesh_terms: D000328:Adult; D050882:CREB-Binding Protein / Q000096:biosynthesis / Q000235:genetics; D045744:Cell Line, Tumor; D002648:Child; D015536:Down-Regulation; D005260:Female; D020869:Gene Expression Profiling; D015973:Gene Expression Regulation, Leukemic; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D054218:Precursor T-Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics* / Q000378:metabolism; D034741:RNA, Small Interfering / Q000008:administration & dosage / Q000235:genetics*; D012097:Repressor Proteins / Q000235:genetics*; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000096:biosynthesis / Q000235:genetics; D025521:Tumor Suppressor Proteins / Q000235:genetics*; D015854:Up-Regulation; D051020:bcl-X Protein / Q000096:biosynthesis / Q000235:genetics
chemical_list: C479740:BCL11B protein, human; C494811:BCL2L1 protein, human; D034741:RNA, Small Interfering; D012097:Repressor Proteins; D053221:TNF-Related Apoptosis-Inducing Ligand; C505653:TNFSF10 protein, human; D025521:Tumor Suppressor Proteins; D051020:bcl-X Protein; D050882:CREB-Binding Protein; C494363:CREBBP protein, human