title: Heme oxygenase-1 is associated with wheat salinity acclimation by modulating reactive oxygen species homeostasis.
abstract: Heme oxygenase-1 (HO-1) has been recently identified as an endogenous signaling system in animals. In this study, HO-1 upregulation and its role in acquired salt tolerance (salinity acclimation) were investigated in wheat plants. We discovered that pretreatment with a low concentration of NaCl (25 mmol/L) not only led to the induction of HO-1 protein and gene expression, as well as enhanced HO activity, but also to a salinity acclimatory response thereafter. The effect is specific for HO-1, since the potent HO-1 inhibitor zinc protoporphyrin IX blocks the above cytoprotective actions, and the cytotoxic responses conferred by 200 mmol/L NaCl are reversed partially when HO-1 inducer hemin is added. Heme oxygenase catalytic product, carbon monoxide (CO) aqueous solution pretreatment, mimicked the salinity acclimatory responses. Meanwhile, the CO-triggered re-establishment of reactive oxygen species (ROS) homeostasis was mainly guaranteed by the induction of total and isozymatic activities, or corresponding transcripts of superoxide dismutase, ascorbate peroxidase, and cytosolic peroxidase (POD), as well as the downregulation of NADPH oxidase expression and cell-wall POD activity. A requirement of hydrogen peroxide homeostasis for HO-1-mediated salinity acclimation was also discovered. Taken together, the above results suggest that the upregulation of HO-1 expression was responsible for the observed salinity acclimation through the regulation of ROS homeostasis.
authors.affiliation: College of Life Sciences, Cooperative Demonstration Laboratory of Centrifuge Technique, Nanjing Agricultural University and Beckman Coulter Ltd. Co., Nanjing Agricultural University, China.
authors.forename: Yanjie
authors.initials: Y
authors.lastname: Xie
authors.forename: Weiti
authors.initials: W
authors.lastname: Cui
authors.forename: Xingxing
authors.initials: X
authors.lastname: Yuan
authors.forename: Wenbiao
authors.initials: W
authors.lastname: Shen
authors.forename: Qing
authors.initials: Q
authors.lastname: Yang
mesh_terms: D000064:Acclimatization / Q000187:drug effects; D002248:Carbon Monoxide / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000378:metabolism; D051547:Heme Oxygenase-1 / Q000378:metabolism*; D006706:Homeostasis / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D054712:Salinity; D036226:Seedlings / Q000187:drug effects / Q000254:growth & development; D012965:Sodium Chloride / Q000494:pharmacology; D012996:Solutions; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014908:Triticum / Q000187:drug effects / Q000201:enzymology* / Q000502:physiology*
chemical_list: D016166:Free Radical Scavengers; D017382:Reactive Oxygen Species; D012996:Solutions; D017392:Thiobarbituric Acid Reactive Substances; D012965:Sodium Chloride; D002248:Carbon Monoxide; D006861:Hydrogen Peroxide; D051547:Heme Oxygenase-1