title: Mig1 is involved in mycelial formation and expression of the genes encoding extracellular enzymes in Saccharomycopsis fibuligera A11.
abstract: The MIG1 gene of Saccharomycopsis fibuligera A11 was cloned from its genomic DNA using the degenerated primers and inverse PCR. The MIG1 gene (1152bp, accession number: HM450676) encoded a 384-amino acid protein very similar to Mig1s from other fungi. Besides their highly conserved zinc fingers, the Mig1 proteins displayed short conserved motifs of possible significance in glucose repression. The MIG1 gene in S. fibuligera A11 was disrupted by integrating the HPT (hygromycin B phosphotransferase) gene into ORF (Open Reading Frame) of the MIG1 gene. The disruptant A11-c obtained could grow in the media containing hygromycin and 2-deoxy-d-glucose, respectively. α-Amylase, glucoamylse, acid protease and β-glucosidase production by the disruptant and expression of their genes in the disruptant were greatly enhanced. This confirms that Mig1, the transcriptional repressor, indeed regulates expression of the genes and production of the extracellular enzymes in S. fibuligera A11. At the same time, it was found that cell budding was enhanced and mycelial formation was reduced in the disruptant.
authors.affiliation: UNESCO Chinese Center of Marine Biotechnology and Institute of Biodiversity and Evolution, Ocean University of China, Yushan Road No. 5, Qingdao, China.
authors.forename: Guang-Lei
authors.initials: GL
authors.lastname: Liu
authors.forename: Dong-Sheng
authors.initials: DS
authors.lastname: Wang
authors.forename: Ling-Fei
authors.initials: LF
authors.lastname: Wang
authors.forename: Shou-Feng
authors.initials: SF
authors.lastname: Zhao
authors.forename: Zhen-Ming
authors.initials: ZM
authors.lastname: Chi
mesh_terms: D000595:Amino Acid Sequence; D000681:Amylases / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D005110:Extracellular Space / Q000201:enzymology* / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D008969:Molecular Sequence Data; D025282:Mycelium / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000235:genetics / Q000378:metabolism; D012442:Saccharomycopsis / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D001617:beta-Glucosidase / Q000235:genetics / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D000681:Amylases; D001617:beta-Glucosidase; D010447:Peptide Hydrolases