title: [Construction of cheA insertion mutant of Campylobacter jejuni and the effect of its adhesion on mice jejunum].
abstract: OBJECTIVE
  To construct cheA (chemotaxis, che) insertion mutant of Campylobacter jejuni and to observe the role of cheA gene in adhesion of Campylobacter jejuni on mice jejunum.
  
  
  METHODS
  We generated cheA gene insertion mutant of C. jejuni NCTC11168 based on homologous recombination. The cheA mutant was checked by PCR and sequencing. We detected the difference in mice jejunal adhesion between cheA mutant and wild-type of C. jejuni NCTC11168 by CFU (Colony-Forming Units) counting of C. jejuni in jejunal content. We confirmed the of role cheA gene plays in mice jejunal adhesion of Campylobacter jejuni by complementation analysis.
  
  
  RESULTS
  PCR results reveal that we have successfully constructed cheA insertion mutant of C. jejuni NCTC11168. The cheA mutant displayed significantly attenuated colonization on jejunal mucosa of mice compared to wild-type strain (P < 0.05). Complementation analysis shows that the complementation of cheA mutant regained its ability in colonization on jejunal mucosa of mice.
  
  
  CONCLUSIONS
  The cheA mutant and its complementation were successfully constructed. The cheA gene may play an important role in colonization of C. jejuni on jejunal mucosa of mice.
authors.affiliation: Clinical Laboratory, First People Hospital of Chang Shu, Changshu 215500, China. 275342137@qq.com
authors.forename: Lili
authors.initials: L
authors.lastname: Xie
authors.forename: Song
authors.initials: S
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Yao
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D001483:Base Sequence; D002169:Campylobacter Infections / Q000382:microbiology*; D016123:Campylobacter jejuni / Q000201:enzymology* / Q000235:genetics* / Q000502:physiology; D006801:Humans; D007583:Jejunum / Q000382:microbiology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D011494:Protein Kinases / Q000235:genetics* / Q000378:metabolism
chemical_list: D011494:Protein Kinases