title: Identification and characterization of 18 novel polymorphic microsatellite makers derived from expressed sequence tags in the Pacific oyster Crassostrea gigas.
abstract: We report the development of 18 new polymorphic microsatellite DNA markers derived from Crassostrea gigas expressed sequences tags. Genotyping of 48 wild adult oysters sampled from Marennes-Oléron bay (France) revealed 12 to 48 alleles per locus. Observed and expected heterozygosity levels ranged from 0.64 to 1 and from 0.77 to 0.97, respectively. The development of these new markers creates a useful complementary tool for population genetics studies, parentage analysis and mapping in Pacific oyster, a species of major aquacultural and ecological importance.
authors.affiliation: Ifremer, Laboratoire de Génétique et Pathologie, La Tremblade, France.
authors.forename: C
authors.initials: C
authors.lastname: Sauvage
authors.forename: P
authors.initials: P
authors.lastname: Boudry
authors.forename: S
authors.initials: S
authors.lastname: Lapègue