title: Constitutive expression of C-myc in nih 3t3 cells which coexpress the colony stimulating factor-I receptor and the catalytic domain of the gtpase-activating protein restores colony stimulating factor-I dependent mitogenic growth.
abstract: To begin to understand how ras p21 might be coupled to the human colony stimulating factor-1 receptor (CSF-IR), we compared the signaling properties of a CSF-IR containing a point mutation that converts the tyrosine at position 809 to a phenylalanine residue to signal transduction in cells that co-express the wild-type receptor and the catalytic domain of the GTPase activating protein (GAP(C)). We find that modulation of the immediate early gene c-myc and a delayed early response represented by the retrotransposon NVL3 were selectively impaired in both situations. Further, constitutive expression of c-myc in cells that contain GAP(C) restored CSF-1-dependent signal transduction. The data suggest a model in which information important for mitogenic cell.growth flows from the region of the receptor containing tyrosine 809, through ras p21, to c-myc in the nucleus.
authors.affiliation: DUKE UNIV,MED CTR,DEPT MICROBIOL & IMMUNOL,BOX 3020,DURHAM,NC 27710.
authors.forename: D
authors.initials: D
authors.lastname: Bortner
authors.forename: M
authors.initials: M
authors.lastname: Ulivi
authors.forename: M
authors.initials: M
authors.lastname: Ostrowski