title: Production of xylooligosaccharides by xylanase from Pichia stipitis based on xylan preparation from triploid Populas tomentosa.
abstract: Xylooligosaccharides (XOS) with DP 2-4 are important synbiotics used as food ingredients based on its prebiotic characteristics. In this work, the production of XOS from lignocellulosic material was performed by combined chemical-enzymatic methods. Xylan was prepared from triploid Populas tomentosa, and bioconverted into XOS by crude xylanase solution obtained from Pichia stipitis. The effects of reaction time, temperature, enzyme dosage, and pH value on the production of XOS were fully evaluated. Under the optimal condition (25U g(-1) substrate, pH 5.4 and 50°C), 36.8% of the xylan preparation was converted to XOS, equivalent to 3.95 mg/mL of the hydrolyzate. Xylobiose, xylotriose and xylotetrose were analyzed to be the main products of the enzymatic hydrolyzate, which together accounted for over 95% of the released oligosaccharides. Meanwhile, the effect of sonication pretreatment on the conversion efficiency of the xylan preparation was also investigated.
authors.affiliation: Institute of Biomass Chemistry and Technology, College of Material Science and Technology, Beijing Forestry University, Beijing, 100083, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Yang
authors.forename: Kun
authors.initials: K
authors.lastname: Wang
authors.forename: Xianliang
authors.initials: X
authors.lastname: Song
authors.forename: Feng
authors.initials: F
authors.lastname: Xu
mesh_terms: D055598:Chemical Phenomena; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008970:Molecular Weight; D009844:Oligosaccharides / Q000096:biosynthesis*; D010843:Pichia / Q000201:enzymology*; D032107:Populus / Q000737:chemistry*; D013010:Sonication; D013696:Temperature; D057885:Triploidy; D014990:Xylans / Q000096:biosynthesis* / Q000737:chemistry
chemical_list: D009844:Oligosaccharides; D014990:Xylans; D043364:Endo-1,4-beta Xylanases