title: Correlation between influence of polysaccharides on hydrolase activity and their antiviral effect in tobacco leaves.
abstract: The activities of hydrolases (acid phosphatase, RNase, and proteases) in healthy and tobacco mosaic virus-infected leaves of Nicotiana tabacum L. var. Samsun, both untreated and treated with polysaccharides (PS) (1,3;1,6-β-D-glucan, fucoidan, and κ/β-carrageenan), were determined. The PS lead to substantial increase in the hydrolase level. The percentage of viral particles undergoing destructive change also increases in leaves treated with PS 24 h before infection. We suppose that the PS-mediated hydrolase activation promotes intracellular destruction of the viral particles and, thus, comprises one of the PS-induced protective mechanisms limiting intracellular viral accumulation.
authors.affiliation: Pacific Institute of Bioorganic Chemistry, Far East Branch of the Russian Academy of Sciences, Vladivostok, Russia.
authors.forename: L A
authors.initials: LA
authors.lastname: Lapshina
authors.forename: V P
authors.initials: VP
authors.lastname: Nagorskaya
authors.forename: A V
authors.initials: AV
authors.lastname: Reunov
authors.forename: A O
authors.initials: AO
authors.lastname: Barabanova
authors.forename: N M
authors.initials: NM
authors.lastname: Shevchenko
authors.forename: I M
authors.initials: IM
authors.lastname: Yermak
authors.forename: T N
authors.initials: TN
authors.lastname: Zvyagintseva
authors.forename: L A
authors.initials: LA
authors.lastname: Elyakova
mesh_terms: D002351:Carrageenan / Q000494:pharmacology; D004789:Enzyme Activation; D005936:Glucans / Q000494:pharmacology; D006867:Hydrolases / Q000378:metabolism*; D016253:Microscopy, Immunoelectron; D010316:Particle Size; D018515:Plant Leaves / Q000201:enzymology / Q000276:immunology / Q000821:virology*; D011134:Polysaccharides / Q000494:pharmacology*; D014026:Nicotiana / Q000187:drug effects* / Q000201:enzymology / Q000821:virology; D014027:Tobacco Mosaic Virus / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D014771:Virion / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D005936:Glucans; D011134:Polysaccharides; C033671:epiglucan; D002351:Carrageenan; C007789:fucoidan; D006867:Hydrolases