title: Visualization of sialic acid produced on bacterial cell surfaces by lectin staining.
abstract: Oligosaccharides containing N-acetylneuraminic acid on the cell surface of some pathogenic bacteria are important for host-microbe interactions. N-acetylneuraminic acid (Neu5Ac) plays a major role in the pathogenicity of bacterial pathogens. For example, cell surface sialyloligosaccharide moieties of the human pathogen Haemophilus influenzae are involved in virulence and adhesion to host cells. In this study, we have established a method of visualizing Neu5Ac linked to a glycoconjugate on the bacterial cell surface based on lectin staining. Photobacterium damselae strain JT0160, known to produce a-2,6-sialyltransferase, was revealed to possess Neu5Ac by HPLC. Using the strain, a strong Sambucus sieboldiana lectin-binding signal was detected. The bacteria producing α-2,6-sialyltransferases could be divided into two groups: those with a lot of α-2,6-linked Neu5Ac on the cell surface and those with a little. In the present study, we developed a useful method for evaluating the relationship between Neu5Ac expression on the cell surface and the degree of virulence of marine bacteria.
authors.affiliation: Glycotechnology Business Unit, Japan Tobacco Inc., 700 Higashibara, Iwata, Shizuoka 438–0802, Japan.
authors.forename: Hitomi
authors.initials: H
authors.lastname: Kajiwara
authors.forename: Munetoyo
authors.initials: M
authors.lastname: Toda
authors.forename: Toshiki
authors.initials: T
authors.lastname: Mine
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nakada
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Wariishi
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yamamoto
mesh_terms: D019158:N-Acetylneuraminic Acid / Q000032:analysis*; D010776:Photobacterium / Q000737:chemistry*; D037121:Plant Lectins / Q000378:metabolism*; D011485:Protein Binding; D054788:Ribosome Inactivating Proteins / Q000378:metabolism*; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D037121:Plant Lectins; C050600:Sambucus nigra lectins; D054788:Ribosome Inactivating Proteins; D019158:N-Acetylneuraminic Acid