title: Development of 52 new polymorphic microsatellite markers for the olive flounder, Paralichthys olivaceus.
abstract: We characterized 52 new microsatellite markers isolated from (GT)(n) and (CT)(n) microsatellite-enriched genomic libraries of the olive flounder (Paralichthys olivaceus). All markers were polymorphic, with eight to 30 (mean 15.1) alleles detected in 30 individuals from a single natural population. Observed heterozygosity ranged from 0.20 to 1.0. Segregation analysis within a mapping family revealed non-amplifying null alleles at six loci. These results indicate that these new microsatellite markers will be useful for population genetic, parentage, and genome mapping studies.
authors.affiliation: Biotechnology Research Institute, National Fisheries Research and Development Institute, Busan 619-705, South Korea Genetics and Breeding Research Center, National Fisheries Research and Development Institute, Geoje 656-842, South Korea Department of Microbiology, Pukyung National University, Busan 608-737, South Korea Department of Molecular Biology, Pusan National University, Busan 609-735, South Korea.
authors.forename: Woo-Jin
authors.initials: WJ
authors.lastname: Kim
authors.forename: Jung-Ha
authors.initials: JH
authors.lastname: Kang
authors.forename: Bo-Hye
authors.initials: BH
authors.lastname: Nam
authors.forename: Hee Jeong
authors.initials: HJ
authors.lastname: Kong
authors.forename: Eun-Mi
authors.initials: EM
authors.lastname: Park
authors.forename: Jeong-Ho
authors.initials: JH
authors.lastname: Lee
authors.forename: Kyung-Kil
authors.initials: KK
authors.lastname: Kim
authors.forename: Young-Ok
authors.initials: YO
authors.lastname: Kim
authors.forename: Hyon Sob
authors.initials: HS
authors.lastname: Han
authors.forename: Tae-Jin
authors.initials: TJ
authors.lastname: Choi
authors.forename: Jaehun
authors.initials: J
authors.lastname: Cheong