title: Construction of a Saccharomyces cerevisiae strain with a high level of RNA.
abstract: A strategy has been developed for creating Saccharomyces cerevisiae strains with a high RNA content by following a three-step breeding procedure. In the first step, an S. cerevisiae disruptant of the RRN10 gene, one of the components of the UAF (upstream activation factor) complex of rRNA transcription, was constructed and showed severely slow growth. In the second step, seven suppressors were isolated that restored the slow growth of the Δrrn10 disruptant. Genetic analysis revealed that each of the seven suppressors that were isolated appeared to have dominant and multiple mutations. The specific growth rate of those suppressors was increased approximately two-fold as compared with the Δrrn10 parental strain. The absolute RNA content showed that the suppressors had an RNA content 32-56% higher than that of the Δrrn10 parental strain. In the last step, the RRN10 wild-type gene was integrated into chromosome V of each of the original suppressors. The total RNA content of the integrants was also 1.4- to 2.3-fold higher than the wild-type strain. In conclusion, since yeast RNA is the source of 5'-IMP and 5'-GMP that enhance the delicious taste in certain types of food, like soups and sauces, the strategy taken in this study provides effective approach to breed S. cerevisiae strains producing a higher content of RNA that will contribute to yeast food biotechnology.
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita-shi, Osaka 565-0871, Japan.
authors.forename: Varesa
authors.initials: V
authors.lastname: Chuwattanakul
authors.forename: Yeon-Hee
authors.initials: YH
authors.lastname: Kim
authors.forename: Minetaka
authors.initials: M
authors.lastname: Sugiyama
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Nishiuchi
authors.forename: Haruhumi
authors.initials: H
authors.lastname: Miwa
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Kaneko
authors.forename: Satoshi
authors.initials: S
authors.lastname: Harashima
mesh_terms: D001709:Biotechnology; D004275:DNA, Ribosomal / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D005800:Genes, Fungal; D030781:Organisms, Genetically Modified / Q000235:genetics*; D012313:RNA / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D004275:DNA, Ribosomal; D004268:DNA-Binding Proteins; C098779:RRN10 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D012313:RNA