title: Derivation of buffalo embryonic stem-like cells from in vitro-produced blastocysts on homologous and heterologous feeder cells.
abstract: OBJECTIVE
  The aim of the present study is to compare the ability of homologous and heterologous embryonic fibroblast feeder layers to support isolation and proliferation of buffalo ES-like cells generated from hatched and expanded blastocysts produced by in vitro fertilization and characterization of derived cells through expression of pluripotent markers.
  
  
  METHODS
  Embryonic stem cells were derived from hatched and expanded blastocysts through intact blastocyst culture and enzymatic method respectively and compared for proliferation rate on homologous (buffalo) and heterologous feeder layers (goat and sheep).
  
  
  RESULTS
  A total of 69 hatched and 83 expanded blastocysts were used for isolation of inner cell masses which were seeded on buffalo, goat and sheep embryonic feeder layers. Following seeding, attachment rate, primary colony formation rate and survival to maximum number of passages were observed to be higher on homologous feeder layers.
  
  
  CONCLUSIONS
  Upon comparison of different feeder layer cells for derivation and maintenance of buffalo ES-like cells from hatched and expanded blastocysts, buffalo embryonic fibroblast cells were able to provide a better environment for maintaining pluripotency in culture conditions.
authors.affiliation: Animal Biotechnology Centre, National Dairy Research Institute, Karnal, 132001, India. vetdmkl@yahoo.co.in
authors.forename: Dharmendra
authors.initials: D
authors.lastname: Kumar
authors.forename: T
authors.initials: T
authors.lastname: Anand
authors.forename: K P
authors.initials: KP
authors.lastname: Singh
authors.forename: M K
authors.initials: MK
authors.lastname: Singh
authors.forename: R A
authors.initials: RA
authors.lastname: Shah
authors.forename: M S
authors.initials: MS
authors.lastname: Chauhan
authors.forename: P
authors.initials: P
authors.lastname: Palta
authors.forename: S K
authors.initials: SK
authors.lastname: Singla
authors.forename: R S
authors.initials: RS
authors.lastname: Manik
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology* / Q000378:metabolism; D002020:Buffaloes / Q000196:embryology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism*; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D061252:Feeder Cells / Q000166:cytology* / Q000378:metabolism; D005307:Fertilization in Vitro / Q000662:veterinary*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D007621:Karyotyping; D012756:Sheep