title: Population dynamics of Crenarchaeota and Euryarchaeota in the mixing front of river and marine waters.
abstract: A transect from the Tomoe River Mouth through Shimizu Port to Suruga Bay, Japan, was examined between 2005 and 2009 to reveal the population dynamics of Crenarchaeota and Euryarchaeota in an estuary environment. Crenarchaeota tended to increase in abundance in waters deeper than 100 m compared with Euryarchaeota, and comprised 11% of total direct counts. Archaeal abundance was highest in the Tomoe River Mouth, with a strong negative correlation between surface euryarchaeal abundance and salinity (P<0.001). The diversity index for the phylotypic archaeal community in the mouth was three times higher than that at sites St1-1m and St1-10m in the estuary, and OTUs represented most of the OTU groups at the sites. Three of the seven total OTUs, which comprised 83.6% of the 140 sequenced clones in the estuary, were related to the OTUs in the mouth with similarities higher than 97%. A significant proportion of the archaeal community appears to be derived from the Tomoe River. The two dominant phylotypes of the archaeal community in Shimizu Port, belonging to MGI and MGII, occurred ubiquitously.
authors.affiliation: Department of Environment and Energy System, Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422–8529, Japan.
authors.forename: Do Manh
authors.initials: do M
authors.lastname: Hao
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Tashiro
authors.forename: Miharu
authors.initials: M
authors.lastname: Kato
authors.forename: Rumi
authors.initials: R
authors.lastname: Sohrin
authors.forename: Tomotaka
authors.initials: T
authors.lastname: Ishibashi
authors.forename: Chie
authors.initials: C
authors.lastname: Katsuyama
authors.forename: Kazuyo
authors.initials: K
authors.lastname: Nagaosa
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kimura
authors.forename: Tran Duc
authors.initials: TD
authors.lastname: Thanh
authors.forename: Kenji
authors.initials: K
authors.lastname: Kato
mesh_terms: D001419:Bacteria / Q000254:growth & development / Q000302:isolation & purification; D001483:Base Sequence; D015169:Colony Count, Microbial; D019615:Crenarchaeota / Q000145:classification / Q000235:genetics / Q000254:growth & development*; D019641:DNA, Archaeal / Q000235:genetics / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004777:Environment; D019605:Euryarchaeota / Q000145:classification / Q000235:genetics / Q000254:growth & development*; D005618:Fresh Water / Q000382:microbiology*; D007564:Japan; D008969:Molecular Sequence Data; D010802:Phylogeny; D011157:Population Dynamics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D045483:Rivers / Q000382:microbiology; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA
chemical_list: D019641:DNA, Archaeal; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S