title: Effects of thermal treatments on protein adsorption of Co-Cr-Mo ASTM-F75 alloys.
abstract: Post-manufacturing thermal treatments are commonly employed in the production of hip replacements to reduce shrinkage voids which can occur in cast components. Several studies have investigated the consequences of these treatments upon the alloy microstructure and tribological properties but none have determined if there are any biological ramifications. In this study the adsorption of proteins from foetal bovine serum (FBS) on three Co-Cr-Mo ASTM-F75 alloy samples with different metallurgical histories, has been studied as a function of protein concentration. Adsorption isotherms have been plotted using the surface concentration of nitrogen as a diagnostic of protein uptake as measured by X-ray photoelectron spectroscopy. The data was a good fit to the Langmuir adsorption isotherm up to the concentration at which critical protein saturation occurred. Differences in protein adsorption on each alloy have been observed. This suggests that development of the tissue/implant interface, although similar, may differ between as-cast (AC) and heat treated samples.
authors.affiliation: Faculty of Engineering and Physical Sciences, University of Surrey, Guildford, Surrey GU27XH, UK.
authors.forename: L A
authors.initials: LA
authors.lastname: Duncan
authors.forename: F H
authors.initials: FH
authors.lastname: Labeed
authors.forename: M-L
authors.initials: ML
authors.lastname: Abel
authors.forename: A
authors.initials: A
authors.lastname: Kamali
authors.forename: J F
authors.initials: JF
authors.lastname: Watts
mesh_terms: D000327:Adsorption; D000497:Alloys / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D004734:Energy Metabolism / Q000502:physiology; D056951:Photoelectron Spectroscopy; D011485:Protein Binding / Q000502:physiology; D011506:Proteins / Q000378:metabolism / Q000493:pharmacokinetics*; D044967:Serum / Q000737:chemistry / Q000378:metabolism; D013696:Temperature; D014800:Vitallium / Q000737:chemistry / Q000378:metabolism*
chemical_list: C105750:ASTM F-75 implant alloy; D000497:Alloys; D011506:Proteins; D014800:Vitallium