title: Isolation and characterization of 13 microsatellite markers for the viviparous surfperch Ditrema temmincki (Embiotocidae) and cross-species amplification.
abstract: Thirteen microsatellite loci were isolated from a size-selected genomic library of the surfperch (Ditrema temmincki Bleeker). All loci displayed a high degree of length polymorphism, as observed in the total number of alleles per locus (two to 23) and a high degree of estimated heterozygosity, ranging from 0.080 to 0.893. The primers developed for D. temmincki were also tested for their ability to amplify homologous sequences in D. viride and Neoditrema ransonetii. Distinct differences were observed among three species of surfperches, in both genetic variability and the frequency distribution of the alleles.
authors.affiliation: Faculty of Agriculture, Ehime University, Tarumi, Matsuyama, 790-8566, Japan, The Research Institute of Marine Bioresources, Fukuyama University, Innoshima, Onomichi, 722-2101, Japan.
authors.forename: Motohiro
authors.initials: M
authors.lastname: Takagi
authors.forename: Kumiko
authors.initials: K
authors.lastname: Sakai
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Taniguchi