title: Antibacterial nano-structured titania coating incorporated with silver nanoparticles.
abstract: Titanium (Ti) implants are widely used clinically but post-operation infection remains one of the most common and serious complications. A surface boasting long-term antibacterial ability is highly desirable in order to prevent implant associated infection. In this study, titania nanotubes (TiO(2)-NTs) incorporated with silver (Ag) nanoparticles are fabricated on Ti implants to achieve this purpose. The Ag nanoparticles adhere tightly to the wall of the TiO(2)-NTs prepared by immersion in a silver nitrate solution followed by ultraviolet light radiation. The amount of Ag introduced to the NTs can be varied by changing processing parameters such as the AgNO(3) concentration and immersion time. The TiO(2)-NTs loaded with Ag nanoparticles (NT-Ag) can kill all the planktonic bacteria in the suspension during the first several days, and the ability of the NT-Ag to prevent bacterial adhesion is maintained without obvious decline for 30 days, which are normally long enough to prevent post-operation infection in the early and intermediate stages and perhaps even late infection around the implant. Although the NT-Ag structure shows some cytotoxicity, it can be reduced by controlling the Ag release rate. The NT-Ag materials are also expected to possess satisfactory osteoconductivity in addition to the good biological performance expected of TiO(2)-NTs. This controllable NT-Ag structure which provides relatively long-term antibacterial ability and good tissue integration has promising applications in orthopedics, dentistry, and other biomedical devices.
authors.affiliation: School of Stomatology, The Fourth Military Medical University, No. 145 West Changle Road, Xi'an 710032, China.
authors.forename: Lingzhou
authors.initials: L
authors.lastname: Zhao
authors.forename: Hairong
authors.initials: H
authors.lastname: Wang
authors.forename: Kaifu
authors.initials: K
authors.lastname: Huo
authors.forename: Lingyun
authors.initials: L
authors.lastname: Cui
authors.forename: Wenrui
authors.initials: W
authors.lastname: Zhang
authors.forename: Hongwei
authors.initials: H
authors.lastname: Ni
authors.forename: Yumei
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zhifen
authors.initials: Z
authors.lastname: Wu
authors.forename: Paul K
authors.initials: PK
authors.lastname: Chu
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D000900:Anti-Bacterial Agents / Q000009:adverse effects / Q000737:chemistry / Q000494:pharmacology*; D002478:Cells, Cultured; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D053768:Metal Nanoparticles / Q000009:adverse effects* / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D043942:Nanotubes / Q000737:chemistry*; D010006:Osteoblasts / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012834:Silver / Q000009:adverse effects / Q000737:chemistry / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects; D014025:Titanium / Q000009:adverse effects / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; C009495:titanium dioxide; D012834:Silver; D014025:Titanium; D007770:L-Lactate Dehydrogenase; D000469:Alkaline Phosphatase