title: [Construction and expression of a coxsackievirus A16 VP1 gene plasmid which delivered by live attenuated Salmonella].
abstract: OBJECTIVE
  To develop a coxsackievirus A16 (Cox A16) VP1 gene plasmid which delivered by live attenuated Salmonella.
  
  
  METHODS
  The plasmid which expressed VP1 protein of CoxA16 was constructed by gene recombination. Cellular expression was assessed by Western bloten analysis. Then the recombinant attenuated Salmonella which harboring the plasmid were constructed by electro transformation.
  
  
  RESULTS
  CoxA16 VP1 gene sequence was inserted into a eukaryotic expression plasmid. VP1 protein was detected in the culture supernatant.
  
  
  CONCLUSIONS
  The plasmid is constructed successfully and it can be expressed effectively in vitro. The recombinant bacteria are constructed successfully. This has provided a basis for further research of an oral CoxA16 vaccine.
authors.affiliation: Military Postgraduate Medical College, Beijing 100853, China. zeer@sohu.com
authors.forename: Ze
authors.initials: Z
authors.lastname: Liu
authors.forename: Bing-ke
authors.initials: BK
authors.lastname: Bai
authors.forename: Rong
authors.initials: R
authors.lastname: Gao
authors.forename: Shao-ping
authors.initials: SP
authors.lastname: Cai
authors.forename: Yan
authors.initials: Y
authors.lastname: Hu
authors.forename: Hong-hui
authors.initials: HH
authors.lastname: Shen
authors.forename: Pan-yong
authors.initials: PY
authors.lastname: Mao
mesh_terms: D001428:Bacterial Vaccines / Q000737:chemistry / Q000235:genetics*; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D004770:Enterovirus / Q000235:genetics*; D010957:Plasmids / Q000737:chemistry / Q000235:genetics*; D012475:Salmonella / Q000737:chemistry / Q000235:genetics*; D014613:Vaccines, Attenuated / Q000138:chemical synthesis / Q000235:genetics*
chemical_list: D001428:Bacterial Vaccines; D036022:Capsid Proteins; C442501:VP1 protein, Foot-and-mouth disease virus; D014613:Vaccines, Attenuated