title: Differences in origin of reactive microglia in bone marrow chimeric mouse and rat after transient global ischemia.
abstract: Current understanding of microglial involvement in disease is influenced by the observation that recruited bone marrow (BM)-derived cells contribute to reactive microgliosis in BM-chimeric mice. In contrast, a similar phenomenon has not been reported for BM-chimeric rats. We investigated the recruitment and microglial transformation of BM-derived cells in radiation BM-chimeric mice and rats after transient global cerebral ischemia, which elicits a characteristic microglial reaction. Both species displayed microglial hyperplasia and rod cell transformation in the hippocampal CA1 region. In mice, a subpopulation of lesion-reactive microglia originated from transformed BM-derived cells. By contrast, no recruitment or microglial transformation of BM-derived cells was observed in BM-chimeric rats. These results suggest that reactive microglia in rats originate from resident microglia, whereas they have a mixed BM-derived and resident origin in mice, depending on the severity of ischemic tissue damage.
authors.affiliation: Institute of Molecular Medicine, Department of Neurobiology Research, University of Southern Denmark, Odense, Denmark.
authors.forename: Kate L
authors.initials: KL
authors.lastname: Lambertsen
authors.forename: Tomas
authors.initials: T
authors.lastname: Deierborg
authors.forename: Rikke
authors.initials: R
authors.lastname: Gregersen
authors.forename: Bettina H
authors.initials: BH
authors.lastname: Clausen
authors.forename: Martin
authors.initials: M
authors.lastname: Wirenfeldt
authors.forename: Helle H
authors.initials: HH
authors.lastname: Nielsen
authors.forename: Ishar
authors.initials: I
authors.lastname: Dalmau
authors.forename: Nils H
authors.initials: NH
authors.lastname: Diemer
authors.forename: Frederik
authors.initials: F
authors.lastname: Dagnaes-Hansen
authors.forename: Flemming F
authors.initials: FF
authors.lastname: Johansen
authors.forename: Armand
authors.initials: A
authors.lastname: Keating
authors.forename: Bente
authors.initials: B
authors.lastname: Finsen
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D000951:Antigens, Neoplasm / Q000235:genetics / Q000378:metabolism; D001853:Bone Marrow / Q000473:pathology*; D001854:Bone Marrow Cells / Q000473:pathology* / Q000502:physiology; D016026:Bone Marrow Transplantation; D039481:CD11b Antigen / Q000378:metabolism; D062507:Claudin-5; D004195:Disease Models, Animal; D005452:Fluoresceins; D049452:Green Fluorescent Proteins / Q000235:genetics; D015395:Histocompatibility Antigens Class I / Q000378:metabolism; D002546:Ischemic Attack, Transient / Q000473:pathology* / Q000503:physiopathology* / Q000601:surgery; D017493:Leukocyte Common Antigens / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D017628:Microglia / Q000502:physiology*; D009930:Organic Chemicals; D051381:Rats; D011917:Rats, Inbred Lew; D017208:Rats, Wistar; D013997:Time Factors
chemical_list: D000951:Antigens, Neoplasm; D039481:CD11b Antigen; D062507:Claudin-5; C491511:Cldn5 protein, mouse; D005452:Fluoresceins; D015395:Histocompatibility Antigens Class I; D008565:Membrane Proteins; D009930:Organic Chemicals; C440529:UBC antigen; C435731:fluoro jade; D049452:Green Fluorescent Proteins; D017493:Leukocyte Common Antigens