title: Quantification of cynaropicrin in artichoke leaf extracts by ¹H NMR spectroscopy.
abstract: The quantification of cynaropicrin in artichoke leaf extracts (ALE) by means of quantitative ¹H NMR was performed. The method relies on solid phase extraction for sample preparation and spectra acquisition in DMSO- D₆ containing anthracene as the internal standard. The obtained ¹H NMR spectra revealed excellent signal purity for H-13(b) of cynaropicrin, which was thus chosen for quantification. Validation was performed in terms of selectivity, linearity, precision, accuracy, and robustness. The content of cynaropicrin in the analyzed samples showed considerable variation, ranging from non detectable to 1.6 %. The NMR approach does not rely on external calibration, which represents the major advantage compared to traditional approaches based on HPLC.
authors.affiliation: Institute of Pharmacy/Pharmacognosy, Center for Molecular Biosciences Innsbruck, Leopold-Franzens University of Innsbruck, Innsbruck, Austria.
authors.forename: Valerio
authors.initials: V
authors.lastname: Pieri
authors.forename: Hermann
authors.initials: H
authors.lastname: Stuppner
mesh_terms: D031943:Cynara scolymus / Q000737:chemistry*; D007783:Lactones / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D010936:Plant Extracts / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D018515:Plant Leaves / Q000737:chemistry; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012717:Sesquiterpenes / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D052616:Solid Phase Extraction / Q000379:methods
chemical_list: D007783:Lactones; D010936:Plant Extracts; D012717:Sesquiterpenes; C075687:cynaropicrin