title: Characterization and expression profile analysis of a sucrose synthase gene from common bean (Phaseolus vulgaris L.) during seed development.
abstract: A full-length cDNA encoding common bean (Phaseolus vulgaris L.) sucrose synthase (designated as Pv_BAT93 Sus), which catalyses the synthesis and cleavage of sucrose, was isolated from seeds at 15 days after pollination (DAP) by rapid amplification of cDNA ends (RACE). The full-length cDNA of Pv_BAT93 Sus had a 2,418 bp open reading frame (ORF) encoding a protein of 806 amino acid residues. Sequence comparison analysis showed that Pv_BAT93 Sus was very similar to several members of the sucrose synthase family of other plant species. Tissue expression pattern analysis showed that Pv_BAT93 Sus was expressed in leaves, flowers, stems, roots, cotyledons, and particularly during seed development. Expression studies using in situ hybridization revealed altered spatial and temporal patterns of Sus expression in the EMS mutant relative to wild-type and confirmed Sus expression in common bean developing seeds. The expression and accumulation of Sus mRNA was clearly shown in several tissues, such as the suspensor and embryo, but also in the transfer cells and endothelium. The results highlight the diverse roles that Sus might play during seed development in common bean.
authors.affiliation: University of Liège-Gembloux Agro-Bio Tech., Unit of Tropical Crop Husbandry and Horticulture, Gembloux Agricultural University, Passage des Déportés 2, 5030 Gembloux, Belgium. gha_abid@yahoo.fr
authors.forename: Ghassen
authors.initials: G
authors.lastname: Abid
authors.forename: Yordan
authors.initials: Y
authors.lastname: Muhovski
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Jacquemin
authors.forename: Dominique
authors.initials: D
authors.lastname: Mingeot
authors.forename: Khaled
authors.initials: K
authors.lastname: Sassi
authors.forename: André
authors.initials: A
authors.lastname: Toussaint
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Baudoin
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D019295:Computational Biology; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D020869:Gene Expression Profiling; D005964:Glucosyltransferases / Q000235:genetics* / Q000378:metabolism*; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D027805:Phaseolus / Q000201:enzymology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012639:Seeds / Q000201:enzymology* / Q000254:growth & development; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D005964:Glucosyltransferases; C022699:sucrose synthase