title: Diverse biological activity of Odontoglossum Harvengtense 'Tutu' bulb extracts.
abstract: BACKGROUND
  Several pharmacologically active substances have been isolated from orchid plants, but not from Odontoglossum Harvengtense 'Tutu'. Whether MeOH extract fractions from Odontoglossum Harvengtense 'Tutu' bulb exert biological activity was investigated.
  
  
  METHODS
  The MeOH extract was stepwise separated by organic solvents into n-hexane, EtOAc, n-BuOH and H(2)O layer fractions. Cytotoxic activity against human tumor and normal cells was determined by MTT method. Nitric oxide (NO) was determined by Griess method. Osteoclastogenesis was monitored by tartrate-resistant acid phosphatase (TRAP) activity.
  
  
  RESULTS
  Among four fractions, the EtOAc fraction showed the highest tumor-specific cytotoxicity, and inhibited NO production by lipopoly-saccharide (LPS)-stimulated mouse macrophage-like cells and receptor activator for nuclear factor-κB ligand (RANKL)-induced osteoclastogenesis to the greatest extent.
  
  
  CONCLUSIONS
  As compared with Odontioda Marie Noel 'Velano' bulbs, the anti-tumor and anti-inflammatory substances of Odontoglossum Harvengtense 'Tutu' are concentrated more exclusively into the EtOAc fraction.
authors.affiliation: Faculty of Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Sakado, Saitama 350-0295, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Masuda
authors.forename: Jun-Ya
authors.initials: JY
authors.lastname: Ueda
authors.forename: Masafumi
authors.initials: M
authors.lastname: Tamura
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sakagami
authors.forename: Mineko
authors.initials: M
authors.lastname: Tomomura
authors.forename: Akito
authors.initials: A
authors.lastname: Tomomura
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Shirataki
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology* / Q000633:toxicity; D001862:Bone Resorption / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D018922:HL-60 Cells; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D029595:Orchidaceae / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology* / Q000633:toxicity; D000071681:Tartrate-Resistant Acid Phosphatase; D014466:Ultraviolet Rays
chemical_list: D000970:Antineoplastic Agents; D007527:Isoenzymes; D010936:Plant Extracts; D009569:Nitric Oxide; D000135:Acid Phosphatase; C000605511:Acp5 protein, mouse; D000071681:Tartrate-Resistant Acid Phosphatase