title: Prenatal LPS exposure reduces olfactory perception in neonatal and adult rats.
abstract: Prenatal lipopolysaccharide (LPS) exposure causes reproductive, behavioral and neurochemical defects in both dams and pups. The present study evaluated male rats prenatally treated with LPS for behavioral and neurological effects related to the olfactory system, which is the main sensorial path in rodents. Pregnant Wistar rats received 100 μg/kg of LPS intraperitoneally (i.p.) on gestational day (GD) 9.5, and maternal behavior was evaluated. Pups were evaluated for (1) maternal odor preference, (2) aversion to cat odor, (3) monoamine levels and turnover in the olfactory bulb (OB) and (4) protein expression (via immunoblotting) within the OB dopaminergic system and glial cells. Results showed that prenatal LPS exposure impaired maternal preference and cat odor aversion and decreased dopamine (DA) levels in the OB. This dopaminergic impairment may have been due to defects in another brain area given that protein expression of the first enzyme in the DA biosynthetic pathway was unchanged in the OB. Moreover, there was no change in the protein expression of the DA receptors. The fact that the number of astrocytes and microglia was not increased suggests that prenatal LPS did not induce neuroinflammation in the OB. Furthermore, given that maternal care was not impaired, abnormalities in the offspring were not the result of reduced maternal care.
authors.affiliation: Department of Pathology, School of Veterinary Medicine, University of São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, 05508-270, São Paulo, SP, Brazil. thik@hotmail.com
authors.forename: Thiago Berti
authors.initials: TB
authors.lastname: Kirsten
authors.forename: Gabriela Pena
authors.initials: GP
authors.lastname: Chaves
authors.forename: Marina
authors.initials: M
authors.lastname: Taricano
authors.forename: Daniel Oliveira
authors.initials: DO
authors.lastname: Martins
authors.forename: Jorge Camilo
authors.initials: JC
authors.lastname: Flório
authors.forename: Luiz Roberto Giorgetti de
authors.initials: LR
authors.lastname: Britto
authors.forename: Andrea da Silva
authors.initials: AS
authors.lastname: Torrão
authors.forename: João
authors.initials: J
authors.lastname: Palermo-Neto
authors.forename: Maria Martha
authors.initials: MM
authors.lastname: Bernardi
mesh_terms: D000367:Age Factors; D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D015306:Biogenic Monoamines / Q000378:metabolism; D039481:CD11b Antigen / Q000378:metabolism; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D008070:Lipopolysaccharides / Q000633:toxicity*; D008297:Male; D008425:Maternal Behavior / Q000187:drug effects; D009812:Odorants; D009830:Olfactory Bulb / Q000378:metabolism; D055696:Olfactory Perception / Q000187:drug effects*; D010468:Perceptual Disorders / Q000209:etiology* / Q000473:pathology; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000139:chemically induced / Q000503:physiopathology*; D051381:Rats; D011954:Receptors, Dopamine / Q000378:metabolism
chemical_list: D015306:Biogenic Monoamines; D039481:CD11b Antigen; D005904:Glial Fibrillary Acidic Protein; D008070:Lipopolysaccharides; D011954:Receptors, Dopamine