title: [Combined effect of fluoride and aluminum on the expression of Runx2 and Osterix mRNA in MC3T3-E1 cells].
abstract: OBJECTIVE
  To study the combined effect of fluoride and aluminum on cell proliferation and differentiation as well as on the expression of Runx2 and Osterix mRNA in osteoblastic cell line.
  
  
  METHODS
  MC3T3-E1 cells were cultured in medium containing 50 micromol/L NaF or/and 5 micromol/L AlCl3. Cell proliferation and differentiation was examined and the expression level of Runx2 and Osterix mRNA were determined by RT-PCR.
  
  
  RESULTS
  Compared with the control group, cell proliferation and differentiation and the expression of Runx2 and Osterix mRNA in MC3T3-E1 cells were enhanced by fluoride and aluminum (P < 0.05). There was a synergistic effect of fluoride and aluminum on the expression of Runx2 and Osterix mRNA in MC3T3-E1 cells.
  
  
  CONCLUSIONS
  Bone formation was promoted by fluoride and aluminum in stimulating the proliferation and differentiation of osteoblastic cells and the expression of Runx2 end Osterix mRNA in the cells.
authors.affiliation: Department of Biogeochemical Disease, School of Public Health, China Medical University, Shenyang 110001, China. xyguo@mail.cmu.edu.cn
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Guo
authors.forename: Ruoxin
authors.initials: R
authors.lastname: Cai
authors.forename: Sisi
authors.initials: S
authors.lastname: Wu
authors.forename: Yang
authors.initials: Y
authors.lastname: He
authors.forename: Guifan
authors.initials: G
authors.lastname: Sun
mesh_terms: D000535:Aluminum / Q000494:pharmacology*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics / Q000378:metabolism*; D004357:Drug Synergism; D005459:Fluorides / Q000494:pharmacology*; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D000076182:Sp7 Transcription Factor; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D050659:Core Binding Factor Alpha 1 Subunit; D012333:RNA, Messenger; C493716:Runx2 protein, mouse; D000076182:Sp7 Transcription Factor; C479466:Sp7 protein, mouse; D014157:Transcription Factors; D000535:Aluminum; D005459:Fluorides