title: Characterization of 15 polymorphic microsatellite markers in the freshwater snail Aplexa marmorata (Mollusca, Gastropoda).
abstract: We describe 15 new variable microsatellites in the freshwater snail Aplexa (Physa) marmorata, as well as conditions for multiplexing and simultaneous genotyping sets of loci. Two to 11 alleles were detected per locus over the five populations studied, and up to eight alleles per locus and population. The observed heterozygosity was extremely low, most probably as the consequence of high selfing rates. The genetic differentiation among populations was large. These markers will constitute efficient tools for investigating the population structure in fragmented habitats. Cross-species amplification in three Physa species (P. acuta, P. fontinalis and P. gyrina) was rather unsuccessful.
authors.affiliation: Centre d'Ecologie Fonctionnelle et Evolutive, UMR 5175 CNRS, 1919 route de Mende, 34293 Montpellier cedex 5, France.
authors.forename: Marie-Pierre
authors.initials: MP
authors.lastname: Dubois
authors.forename: Antoine
authors.initials: A
authors.lastname: Nicot
authors.forename: Philippe
authors.initials: P
authors.lastname: Jarne
authors.forename: Patrice
authors.initials: P
authors.lastname: David