title: A multipoint hydrogen-bond network underlying KcsA C-type inactivation.
abstract: In the prokaryotic potassium channel KcsA activation gating at the inner bundle gate is followed by C-type inactivation at the selectivity filter. Entry into the C-type inactivated state has been directly linked to the strength of the H-bond interaction between residues Glu-71 and Asp-80 behind the filter, and is allosterically triggered by the rearrangement of the inner bundle gate. Here, we show that H-bond pairing between residues Trp-67 and Asp-80, conserved in most K⁺ channels, constitutes another critical interaction that determines the rate and extent of KcsA C-type inactivation. Disruption of the equivalent interaction in Shaker (Trp-434-Asp-447) and Kv1.2 (Trp-366-Asp-379) leads also to modulation of the inactivation process, suggesting that these residues also play an analogous role in the inactivation gating of Kv channels. The present results show that in KcsA C-type inactivation gating is governed by a multipoint hydrogen-bond network formed by the triad Trp-67-Glu71-Asp-80. This triad exerts a critical role in the dynamics and conformational stability of the selectivity filter and might serve as a general modulator of selectivity filter gating in other members of the K⁺ channel family.
authors.affiliation: Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, Illinois, USA.
authors.forename: Julio F
authors.initials: JF
authors.lastname: Cordero-Morales
authors.forename: Vishwanath
authors.initials: V
authors.lastname: Jogini
authors.forename: Sudha
authors.initials: S
authors.lastname: Chakrapani
authors.forename: Eduardo
authors.initials: E
authors.lastname: Perozo
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution / Q000235:genetics; D000818:Animals; D001224:Aspartic Acid / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D006860:Hydrogen Bonding; D015640:Ion Channel Gating; D008958:Models, Molecular; D008969:Molecular Sequence Data; D050505:Mutant Proteins / Q000737:chemistry / Q000378:metabolism; D015221:Potassium Channels / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D051381:Rats; D014364:Tryptophan / Q000378:metabolism; D014981:Xenopus
chemical_list: D001426:Bacterial Proteins; D050505:Mutant Proteins; D015221:Potassium Channels; C107790:prokaryotic potassium channel; D001224:Aspartic Acid; D014364:Tryptophan