title: Characterization of discriminatory urinary proteomic biomarkers for severe preeclampsia using SELDI-TOF mass spectrometry.
abstract: To analyze the proteomic pattern in urine for distinguishing severe preeclampsia from mild preeclampsia and normotensive controls using surface-enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI-TOF-MS).
  
  
  
  Urine samples were collected from women with severe preeclampsia (n=11 [sPE]), mild preeclampsia (n=7 [mPE]), and normotensive controls (n=8) and analyzed by SELDI-TOF-MS to identify discriminatory protein peaks in the sPE cohort. A scoring system was constructed--designated as Preeclampsia Proteomic Score of Urine (PPSU)--to differentiate sPE from mPE and normotensive controls.
  
  
  
  Four discriminatory protein peaks were identified (m/z ratio: 4155, 6044, 6663, and 7971), all of which were down-regulated in women with sPE. PPSU scores in women with sPE were significantly lower than that in both mPE and controls (sPE 0 [0-4] vs. mPE 3 [0-4] vs. controls 4 [2-4]; median [range]; P<0.05). PPSU<2 had a sensitivity of 90.9% and specificity of 93.3% in discriminating patients with sPE from mPE and controls.
  
  
  
  Proteomic analysis of urine can accurately distinguish sPE from mPE and normotensive controls.
authors.affiliation: Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Seung Mi
authors.initials: SM
authors.lastname: Lee
authors.forename: Joong Shin
authors.initials: JS
authors.lastname: Park
authors.forename: Errol R
authors.initials: ER
authors.lastname: Norwitz
authors.forename: Sun Min
authors.initials: SM
authors.lastname: Kim
authors.forename: Byoung Jae
authors.initials: BJ
authors.lastname: Kim
authors.forename: Chan-Wook
authors.initials: CW
authors.lastname: Park
authors.forename: Jong Kwan
authors.initials: JK
authors.lastname: Jun
authors.forename: Hee Chul
authors.initials: HC
authors.lastname: Syn
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000652:urine*; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D011225:Pre-Eclampsia / Q000175:diagnosis / Q000652:urine*; D011247:Pregnancy; D020543:Proteome / Q000032:analysis; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D015415:Biomarkers; D020543:Proteome