title: Human decidua and invasive trophoblasts are rich sources of nearly all human matrix metalloproteinases.
abstract: Trophoblast cell (CTB) invasion into the maternal endometrium plays a crucial role during human embryo implantation and placentation. As for all invasive cell types, the ability of CTB to infiltrate the uterine wall is facilitated by the activity of matrix metalloproteinases (MMPs), which is regulated by tissue inhibitors of MMPs (TIMPs). There is evidence for the expression of several MMPs and TIMPs in decidua. However, published data are limited. Therefore, to set a foundation for future research, we screened a panel of healthy human deciduas obtained during first, second and third trimester of pregnancy in addition to isolated decidual cell populations for the expression of all known human MMPs and TIMPs by RT-PCR, western blot and immunohistochemistry. In the decidual samples, we detected almost all MMPs and all four TIMPs at mRNA level. While the expression of proMMP-3 and active MMP-13 and -23 was down-regulated in the course of pregnancy, the pro forms of MMP-8, -19 and -23, active MMP-9, -10, -12, -15, -16, -26 and -28, and pro- and active MMP-14 increased towards the end of gestation. All MMPs and TIMPs were expressed in uterine natural killer cells, decidual fibroblasts and/or trophoblasts, with the exception of MMP-20 and -25. In summary, a remarkably broad spectrum of MMPs was expressed at the human feto-maternal interface, reflecting the highly invasive and remodelling effect on placenta formation. It can be speculated that expression of MMPs correlates with the invasive potential of CTBs together with a crucial role in activation of labour at term.
authors.affiliation: Department of Obstetrics and Gynaecology, University Hospital of Würzburg, Germany. stojic_j@klinik.uni-wuerzburg.de
authors.forename: Jelena
authors.initials: J
authors.lastname: Anacker
authors.forename: Sabine E
authors.initials: SE
authors.lastname: Segerer
authors.forename: Carsten
authors.initials: C
authors.lastname: Hagemann
authors.forename: Sonja
authors.initials: S
authors.lastname: Feix
authors.forename: Michaela
authors.initials: M
authors.lastname: Kapp
authors.forename: Renate
authors.initials: R
authors.lastname: Bausch
authors.forename: Ulrike
authors.initials: U
authors.lastname: Kämmerer
mesh_terms: D003656:Decidua / Q000166:cytology / Q000201:enzymology* / Q000378:metabolism; D010064:Embryo Implantation; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D007694:Killer Cells, Natural / Q000378:metabolism; D020782:Matrix Metalloproteinases / Q000378:metabolism*; D010920:Placenta / Q000378:metabolism; D010929:Placentation / Q000502:physiology; D011247:Pregnancy; D012333:RNA, Messenger / Q000096:biosynthesis; D019714:Tissue Inhibitor of Metalloproteinases / Q000378:metabolism*; D014327:Trophoblasts / Q000201:enzymology* / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D019714:Tissue Inhibitor of Metalloproteinases; D020782:Matrix Metalloproteinases