title: A study of the impact of cytokine gene polymorphism in acute rejection of renal transplant recipients.
abstract: Acute rejection is a common phenomenon in transplantation. Inflammatory and anti-inflammatory mediators affect the graft microenvironment. Th1 responses cause acute rejection while Th2 immune responses help the survival of the graft. In this study, we evaluated gene polymorphisms of IL-6 G-174C, TGF-β T+869C, IL-4 C-590T, and IFN-γ T+874A cytokines in renal transplant patients. ARMS-PCR method was used to characterize IL-6 G-174C (rs76144090), TGF-β T+869C (rs1800471), and IFN-γ T+874A (rs2430561) polymorphisms and PCR-RFLP, for characterization of IL-4 C-590T (rs2243250) in 100 renal transplant patients. Acute rejection episodes were diagnosed according to the standard criteria. Analysis of the results showed that IL-6-174 GG genotype (P=0.018, OR=3.023, 95% CI=1.183-7.726) and IL-6-174G allele (P=0.046, OR=2.114, 95% CI=1.005-4.447) were more frequent, but IL-6-174GC genotype was less frequent in acute rejection of kidney transplantation in comparison with control group (P=0.024, OR=0.302, 95% CI=0.103-0.883). IFN-γ+874 T allele was associated with a higher risk of acute rejection (P=0.019, OR=2.088, 95% CI=1.124-3.880) while IFN-γ+874 AA genotype was associated with a lower risk of rejection (P=0.023, OR=0.318, 95% CI=0.115-0.875). Frequencies of TGF-β T+869C and IL-4 C-590T were not significantly different (P>0.05). Consequently, our results show that IL-6 G-174C and IFN-γ T+874A gene polymorphisms have predictive values for acute rejection after renal transplantation in Iranian patients.
authors.affiliation: Transplant Research Center, Shiraz University of Medical Sciences, and Transplant Center, Namazi Hospital, Shiraz, Iran. Karimimh@sums.ac.ir
authors.forename: Mohammad Hossein
authors.initials: MH
authors.lastname: Karimi
authors.forename: Saeed
authors.initials: S
authors.lastname: Daneshmandi
authors.forename: Ali Akbar
authors.initials: AA
authors.lastname: Pourfathollah
authors.forename: Bita
authors.initials: B
authors.lastname: Geramizadeh
authors.forename: Ramin
authors.initials: R
authors.lastname: Yaghobi
authors.forename: Ghanbar Ali
authors.initials: GA
authors.lastname: Rais-Jalali
authors.forename: Jamshid
authors.initials: J
authors.lastname: Roozbeh
authors.forename: Shahram
authors.initials: S
authors.lastname: Bolandparvaz
mesh_terms: D000328:Adult; D016207:Cytokines / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006084:Graft Rejection / Q000235:genetics*; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics; D015847:Interleukin-4 / Q000235:genetics; D015850:Interleukin-6 / Q000235:genetics; D007492:Iran; D016030:Kidney Transplantation; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D016212:Transforming Growth Factor beta / Q000235:genetics
chemical_list: D016207:Cytokines; D017931:DNA Primers; C508598:IL4 protein, human; C508600:IL6 protein, human; D015850:Interleukin-6; D016212:Transforming Growth Factor beta; D015847:Interleukin-4; D007371:Interferon-gamma