title: Comparative analysis of bacterial and archaeal communities in methanogenic sludge granules from upflow anaerobic sludge blanket reactors treating various food-processing, high-strength organic wastewaters.
abstract: A comprehensive survey of bacterial and archaeal community structures within granular sludges taken from twelve different types of full-scale, food-processing wastewater-treating, upflow anaerobic sludge blanket (UASB) reactors was performed with a 16S rRNA gene-based clone library method. In total, 1,282 bacterial 16S rRNA gene clones and 722 archaeal clones were analyzed, and their identities were determined by phylogenetic analyses. Overall, clones belonging to the bacterial phyla Proteobacteria (the class Deltaproteobacteria in particular), Firmicutes, Spirochaetes, and Bacteroidetes were observed in abundance within the bacterial clone libraries examined, indicating common bacterial denominators in such treatment systems. Within the domain Archaea, clones affiliated with the classes Methanomicrobia and Methanobacteria were found to be abundant in the archaeal libraries. In relation to features of reactor performance (such as chemical oxygen demand removal, fatty acid accumulation, and sludge bulking), possible representative phylotypes likely to be associated with process failures, such as sludge bulking and the accumulation of propionate, were found in comparative analyses of the distribution of phylotypes in the sludge libraries.
authors.affiliation: Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Narihiro
authors.forename: Takeshi
authors.initials: T
authors.lastname: Terada
authors.forename: Kae
authors.initials: K
authors.lastname: Kikuchi
authors.forename: Akinori
authors.initials: A
authors.lastname: Iguchi
authors.forename: Mizuyo
authors.initials: M
authors.lastname: Ikeda
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Yamauchi
authors.forename: Koji
authors.initials: K
authors.lastname: Shiraishi
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Kamagata
authors.forename: Kazunori
authors.initials: K
authors.lastname: Nakamura
authors.forename: Yuji
authors.initials: Y
authors.lastname: Sekiguchi