title: Anti-inflammatory, antiviral and quantitative study of quercetin-3-O-β-D-glucuronide in Polygonum perfoliatum L.
abstract: Quercetin-3-O-β-D-glucuronide, isolated from Polygonum perfoliatum L., was evaluated by antiviral efficacy against influenza A virus and anti-inflammatory activity in vivo in mouse, and it was used for quality evaluation of P. perfoliatum L.. In vivo study, oral administration of quercetin-3-O-β-D-glucuronide significantly suppressed ear edema induced by dimethyl benzene and peritoneal permeability induced by acetic acid in mice, and quercetin-3-O-β-D-glucuronide also showed to possess inhibitory activity against influenza A virus (FLUAV). In the present study, additionally, a rapid, simple and sensitive method for quantitative analysis of quercetin-3-O-β-D-glucuronide in P. perfoliatum L. was developed using high performance liquid chromatography (HPLC) coupled with photodiode array detection. The separation was carried out on a Lichrosher-C18 column (250 mm × 4.6mm, 5 μm) together with a C18 guard column at isocratic elution systems of methanol (A) and 0.05% aqueous phosphoric acid (B) (43:57, v/v) with detection wavelength at 258 nm and column temperature at 30°C. The method was validated for linearity, repeatability, limit of quantification (LOQ), precision and robustness. The contents of quercetin-3-O-β-D-glucuronide in 28 samples from different regions of China were between 0.06% and 2.09%. The developed analytical method was applied to investigate P. perfoliatum L. and for quality control of the herb.
authors.affiliation: The Research Center for Quality Control of Natural Medicine, Guizhou Normal University, Guiyang 550001, China.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Fan
authors.forename: Xin
authors.initials: X
authors.lastname: Zhou
authors.forename: Chao
authors.initials: C
authors.lastname: Zhao
authors.forename: Huaguo
authors.initials: H
authors.lastname: Chen
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhao
authors.forename: Xiaojian
authors.initials: X
authors.lastname: Gong
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000998:Antiviral Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002199:Capillary Permeability / Q000187:drug effects; D002681:China; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D004487:Edema / Q000188:drug therapy; D009980:Influenza A virus / Q000187:drug effects; D008168:Lung / Q000821:virology; D051379:Mice; D023421:Models, Animal; D028502:Polygonum / Q000737:chemistry*; D011794:Quercetin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D011897:Random Allocation; D015203:Reproducibility of Results
chemical_list: D000893:Anti-Inflammatory Agents; D000998:Antiviral Agents; D004365:Drugs, Chinese Herbal; C443401:quercetin 3-O-glucuronide; D011794:Quercetin