title: Four cleaved amplified polymorphic sequence (CAPS) markers for the detection of the Juglans ailantifolia chloroplast in putatively native J. cinerea populations.
abstract: Hybridization between butternut (Juglans cinerea), a forest tree native to eastern North America, and Japanese walnut (J. ailantifolia), a tree tolerant to the lethal fungal disease butternut canker, casts doubt on the genetic identity of the remaining butternuts. We report a diagnostic test to distinguish the J. cinerea chloroplast from the J. ailantifolia chloroplast using cleaved amplified polymorphic sequences resolvable in 1.5% agarose gels. J. ailantifolia maternal ancestry in naturally regenerated stands provides a site selection criterion for studies of introgression dynamics when the non-native parent and the hybrids tolerate a disease to which the native species is susceptible.
authors.affiliation: Department of Biological Sciences, 327 Galvin Life Sciences, University of Notre Dame, Notre Dame, IN 46556, USA 9825 Bomarc Street, El Paso, TX 79924, USA Department of Plant Pathology and Ecology, Connecticut Agricultural Experiment Station, 123 Huntington Street, PO Box 1106, New Haven, CT 06504-1106, USA Department of Forest Biology, 282 Ellington Plant Sciences Bldg, University of Tennessee, Knoxville, TN 37996, USA.
authors.forename: Tim S
authors.initials: TS
authors.lastname: McCleary
authors.forename: Rodney L
authors.initials: RL
authors.lastname: Robichaud
authors.forename: Steve
authors.initials: S
authors.lastname: Nuanes
authors.forename: Sandra L
authors.initials: SL
authors.lastname: Anagnostakis
authors.forename: Scott E
authors.initials: SE
authors.lastname: Schlarbaum
authors.forename: Jeanne
authors.initials: J
authors.lastname: Romero-Severson