title: Variable region gene analysis of 2 human monoclonal-antibodies to gd3.
abstract: We analyzed the genetic origins of anti-GD3 antibodies by comparing nucleotide sequences of the variable regions from the human monoclonal antibody (mAb), 27-26 (mu, k), established from a patient with leukemia, and another human anti-GD3 mAb, HJM-1 (mu, lambda) derived from a patient with melanoma. The variable regions of 27-26 and HJM-1 were remarkably similar to the germ-line genes. The mAb 27-26 was thought to be derived from germ-line repertoire expanded throughout our experiment. HJM-1 was derived from lymphocytes stimulated by GD3 abundantly expressed on melanoma cells.
authors.affiliation: AICHI RED CROSS BLOOD CTR,SETO 489,JAPAN. NAGASAKI UNIV,SCH MED,DEPT ONCOL,NAGASAKI 852,JAPAN.
authors.forename: T
authors.initials: T
authors.lastname: Ichihashi
authors.forename: H
authors.initials: H
authors.lastname: Yamaguchi
authors.forename: K
authors.initials: K
authors.lastname: Furukawa
authors.forename: H
authors.initials: H
authors.lastname: Shiku
authors.forename: T
authors.initials: T
authors.lastname: Naoe
authors.forename: R
authors.initials: R
authors.lastname: Ohno