title: Human cd4+ cd45ra+ lymphocytes-T can be stimulated by crocidolite, anthophyllite and amosite asbestos invitro.
abstract: Crocidolite, anthophyllite and amosite asbestos stimulate T lymphocyte subsets. Cell surface CD4 or CD45RA expression in peripheral blood mononuclear cells (PBMC) was downregulated temporarily after incubation with asbestos in vitro. The percentage of CD4+ CD45RA+ cells significantly decreased 12 h after incubation with asbestos, suggesting activation of the cells. An early increase in the intracellular Ca++ level was also indicative of activation. An elevated Ca++ level was observed after incubation in both PBMC and purified T cell fractions.
authors.affiliation: KAWASAKI MED UNIV,DEPT HYG,MATSUSHIMA 577,KURASHIKI,OKAYAMA 70101,JAPAN. KAWASAKI MED UNIV,DEPT MED,KURASHIKI,OKAYAMA 70101,JAPAN.
authors.forename: Y
authors.initials: Y
authors.lastname: Watanabe
authors.forename: M
authors.initials: M
authors.lastname: Yamaguchi
authors.forename: Y
authors.initials: Y
authors.lastname: Kawakami
authors.forename: F
authors.initials: F
authors.lastname: Hyodoh
authors.forename: H
authors.initials: H
authors.lastname: Tsushima
authors.forename: G
authors.initials: G
authors.lastname: Ohsawa
authors.forename: A
authors.initials: A
authors.lastname: Ueki