title: Effect of melatonin on the cytotoxicity of chemotherapeutic drugs in human leukemia cells.
abstract: OBJECTIVE
  Limited data are available on the effect of melatonin (MLT) on the cytotoxicity of chemotherapeutic drugs in tumor cells. In this study, we aimed to evaluate the effect of MLT on the cytotoxicity of different chemotherapeutic agents in leukemia cells in vitro.
  
  
  METHODS
  The experiments were carried out using human leukemia cell lines, Jurkat, MOLT-4, Daudi, HL-60, CMK, and K562, and two patient samples. Leukemia cells were incubated with cytarabine, daunorubicin, and etoposide with or without 10(-5) M and 10(-3) M concentrations of MLT. Cytotoxicity was measured by detecting apoptosis using flow cytometry.
  
  
  RESULTS
  Overall, co-incubation with melatonin did not alter the cytotoxicity of chemotherapeutic drugs in cell lines and patient samples except one. In a patient sample with acute myeloid leukemia, etoposide treatment in combination either concentrations of MLT resulted in increased elimination of the leukemia cells.
  
  
  CONCLUSIONS
  Melatonin does not interfere with the cytotoxic effect of cytarabine, daunorubicin and etoposide in leukemia cells.
authors.affiliation: Children's Hospital of Michigan, Division of Hematology/Oncology, Barbara Ann Karmanos Cancer Center, Wayne State University School of Medicine, Detroit, MI 48201, USA.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Büyükavci
authors.forename: Oner
authors.initials: O
authors.lastname: Ozdemir
authors.forename: Steve
authors.initials: S
authors.lastname: Buck
authors.forename: Yaddanapudi
authors.initials: Y
authors.lastname: Ravindranath
authors.forename: Süreyya
authors.initials: S
authors.lastname: Savaşan
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology / Q000633:toxicity*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects*; D004347:Drug Interactions; D018922:HL-60 Cells; D006801:Humans; D019169:Jurkat Cells; D020014:K562 Cells; D007938:Leukemia / Q000473:pathology; D008550:Melatonin / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D008550:Melatonin