title: A viable hypomorphic allele of the essential IMP3 gene reveals novel protein functions in Saccharomyces cerevisiae.
abstract: In Saccharomyces cerevisiae, the essential IMP3 gene encodes a component of the SSU processome, a large ribonucleoprotein complex required for processing of small ribosomal subunit RNA precursors. Mutation of the IMP3 termination codon to a sense codon resulted in a viable mutant allele producing a C-terminal elongated form of the Imp3 protein. A strain expressing the mutant allele displayed ribosome biogenesis defects equivalent to IMP3 depletion. This hypomorphic allele represented a unique opportunity to investigate and better understand the Imp3p functions. We demonstrated that the +1 frameshifting was increased in the mutant strain. Further characterizations revealed involvement of the Imp3 protein in DNA repair and telomere length control, pointing to a functional relationship between both pathways and ribosome biogenesis.
authors.affiliation: IGM, CNRS, UMR 8621, Orsay, France.
authors.forename: Bruno
authors.initials: B
authors.lastname: Cosnier
authors.forename: Marta
authors.initials: M
authors.lastname: Kwapisz
authors.forename: Isabelle
authors.initials: I
authors.lastname: Hatin
authors.forename: Olivier
authors.initials: O
authors.lastname: Namy
authors.forename: Sylvie
authors.initials: S
authors.lastname: Hermann-Le Denmat
authors.forename: Antonin
authors.initials: A
authors.lastname: Morillon
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Rousset
authors.forename: Céline
authors.initials: C
authors.lastname: Fabret
mesh_terms: D000483:Alleles; D004249:DNA Damage; D004260:DNA Repair; D016368:Frameshift Mutation; D015966:Gene Expression Regulation, Fungal; D016681:Genome, Fungal; D008957:Models, Genetic; D009154:Mutation; D011132:Polyribosomes / Q000378:metabolism; D017434:Protein Structure, Tertiary; D012269:Ribosomal Proteins / Q000235:genetics* / Q000502:physiology; D012270:Ribosomes / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000502:physiology; D016615:Telomere / Q000648:ultrastructure; D020798:Two-Hybrid System Techniques
chemical_list: C120533:IMP3 protein, S cerevisiae; D012269:Ribosomal Proteins; D029701:Saccharomyces cerevisiae Proteins