title: Down-regulation of miR-27a might inhibit proliferation and drug resistance of gastric cancer cells.
abstract: OBJECTIVE
  Here we aimed to firstly investigate the role of miR-27a in proliferation and multidrug resistance of gastric cancer cells.
  
  
  METHODS
  The role of miR-27a in gastric cancer cells was detected using MTT assay, soft agar assay, flow cytometry assay, nude mice assay, real-time PCR, western blot and reporter gene assay, etc.
  
  
  RESULTS
  Down-regulation of miR-27a could inhibit porliferation of gastric cancer cells in vitro and in vivo. Down-regulation of miR-27a could also confer sensitivity of drugs on gastric cancer cells, and might increase accumulation and decrease releasing amount of adriamycin in gastric cancer cells. Down-regulation of miR-27a could significantly decrease the expression of P-glycoprotein and the transcriptional activity of cyclin D1, and up-regulate the expression of p21.
  
  
  CONCLUSIONS
  MiR-27a might play important roles in porliferation and drug resistance of gastric cancer. MiR-27a might be considered as a useful target for cancer therapy.
authors.affiliation: Institute of Digestive Disease, Subsidary Hospital, The Medical University of Ningxia Province, Yin'chuan, Ningxia Province, China. ningxiaxiaohong@126.com
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Zhao
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.forename: Jianguo
authors.initials: J
authors.lastname: Hu
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000188:drug therapy / Q000503:physiopathology*; D023041:Xenograft Model Antitumor Assays
chemical_list: C531388:MIRN27 microRNA, human; D035683:MicroRNAs