title: [Study on leptin enhancing collagen systhesis in wounded rats].
abstract: OBJECTIVE
  To investigate the effect of leptin on collagen systhesis in wounded rats.
  
  
  METHODS
  Thirty male Wistar rats, weight (180 +/- 20)g, were randomly divided into three groups (n = 10) by weight: normal depilation group, wound control group and leptin treatment group and ten rats were included in each group. A full-thickness defect measuring 2 x 2.5 cm was made in the back of rats in wound control group and leptin treatment group. Each wound in rats of leptin treatment group was applied topically with 0.1 ml leptin solution (2.0 microg leptin), daily for 7 days and that of wound control group with equivalent saline solution. All rats were killed and then granulation tissues samples and skin were collected to examine the synthesis of collagen.
  
  
  RESULTS
  Hydroxyproline content in granulation tissues of in leptin treatment group (33.92 +/- 3.09) mg/g were significantly increased than those in control group (29.55 +/- 3.59 mg/g, P < 0.05). The mRNA expressions of collagen I and III were significantly enhanced in leptin treatment group (0.96 +/- 0.09, 0.09 +/- 0.06) than those in control group (0.80 +/- 0.03, 0.08 +/- 0.03). The levels of type I and III collagen were significantly increased in leptin treatment group than those in control group.
  
  
  CONCLUSIONS
  Leptin applied topically can accelerate wound healing through enhancing gene expression of type I and III collagen and synthesis of collagen in wound tissue.
authors.affiliation: Institute of Health and Environmental Medicine, Academy of Military Medical Sciences, Tianjin 300050, China.
authors.forename: Pei-Bing
authors.initials: PB
authors.lastname: Li
authors.forename: Hong
authors.initials: H
authors.lastname: Jin
authors.forename: Dian-Xin
authors.initials: DX
authors.lastname: Liu
authors.forename: Yong-Hui
authors.initials: YH
authors.lastname: Wang
authors.forename: Wen-Kao
authors.initials: WK
authors.lastname: Nan
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism*; D024061:Collagen Type III / Q000235:genetics / Q000378:metabolism*; D020738:Leptin / Q000008:administration & dosage / Q000627:therapeutic use*; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014945:Wound Healing / Q000187:drug effects; D014947:Wounds and Injuries / Q000188:drug therapy*
chemical_list: D024042:Collagen Type I; D024061:Collagen Type III; D020738:Leptin; D012333:RNA, Messenger