title: SugE, a new member of the SMR family of transporters, contributes to antimicrobial resistance in Enterobacter cloacae.
abstract: We cloned a gene, sugE, from the chromosome of Enterobacter cloacae ATCC 13047. Analysis of the susceptibilities of the sugE-containing strain (Escherichia coli KAM32/pSUGE28) and sugE-deficient E. cloacae (EcΔsugE) showed that SugE confers resistance to cetyltrimethylammonium bromide, cetylpyridinium chloride, tetraphenylphosphonium, benzalkonium chloride, ethidium bromide, and sodium dodecyl sulfate. We also investigated expression of sugE. We confirm here that SugE from E. cloacae is an SMR family transporter as determined by observing its energy-dependent drug efflux activity.
authors.affiliation: Department of Clinical Laboratory and Nutritional Sciences, University of Massachusetts Lowell, Lowell, MA 01854, USA. Guixin_He@uml.edu
authors.forename: Gui-Xin
authors.initials: GX
authors.lastname: He
authors.forename: Chu
authors.initials: C
authors.lastname: Zhang
authors.forename: Robert R
authors.initials: RR
authors.lastname: Crow
authors.forename: Conner
authors.initials: C
authors.lastname: Thorpe
authors.forename: Huizhong
authors.initials: H
authors.lastname: Chen
authors.forename: Sanath
authors.initials: S
authors.lastname: Kumar
authors.forename: Tomofusa
authors.initials: T
authors.lastname: Tsuchiya
authors.forename: Manuel F
authors.initials: MF
authors.lastname: Varela
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001483:Base Sequence; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D016972:Enterobacter cloacae / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D055786:Gene Knockout Techniques; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D018832:Molecular Chaperones / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion
chemical_list: D000900:Anti-Bacterial Agents; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D018832:Molecular Chaperones; C080403:sugE protein, E coli