title: Tissue-direct PCR, a rapid and extraction-free method for barcoding of ferns.
abstract: Fern gametophytes and young sporophytes often provide too little material for DNA extraction and are particularly difficult to identify to genus. Here we developed an efficient procedure called 'Tissue-direct PCR', in which a slice of fern tissue is mixed with PCR reagents and primers, allowing certain genomic regions to be amplified directly in the thermal cycler. For these diminutive and featureless stages of ferns, Tissue-direct PCR combined with amplifying plant barcodes promises to make the identification of immature ferns easy and rapid. Tissue-direct PCR would also be very helpful for large-scale ecological studies surveying distribution and population structure.
authors.affiliation: Department of Life Science, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 10617, Taiwan Institute of Ecology and Evolutionary Biology, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 10617, Taiwan Division of Forest Biology, Taiwan Forestry Research Institute, 53 Nan-Hai Road, Taipei, 10066, Taiwan.
authors.forename: F-W
authors.initials: FW
authors.lastname: Li
authors.forename: L-Y
authors.initials: LY
authors.lastname: Kuo
authors.forename: Y-M
authors.initials: YM
authors.lastname: Huang
authors.forename: W-L
authors.initials: WL
authors.lastname: Chiou
authors.forename: C-N
authors.initials: CN
authors.lastname: Wang