title: Cytomegalovirus DNA quantification using an automated platform for nucleic acid extraction and real-time PCR assay setup.
abstract: Analytical performance characteristics of the QIAsymphony RGQ system with artus cytomegalovirus (CMV) reagents were determined. Measurable range spanned 2.0 to ≥ 7.0 log(10) copies/ml. The detection limit was 23 copies/ml. Intrarun and interrun coefficients of variation were ≤ 2.1% at 3.0 and 5.0 log(10) copies/ml. In clinical specimens, RGQ values were ~0.2 log(10) copies/ml higher than those in an assay using a BioRobot M48 extraction/manual reaction setup/7500 Real-Time PCR instrument. No cross-contamination was observed.
authors.affiliation: The Johns Hopkins Medical Institutions, Division of Microbiology, Baltimore, Maryland, USA. mformaa@jhmi.edu
authors.forename: Michael
authors.initials: M
authors.lastname: Forman
authors.forename: Andy
authors.initials: A
authors.lastname: Wilson
authors.forename: Alexandra
authors.initials: A
authors.lastname: Valsamakis
mesh_terms: D001331:Automation / Q000379:methods*; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000821:virology*; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019562:Viral Load / Q000379:methods*
chemical_list: D004279:DNA, Viral