title: DOLICHOL PHOSPHATE MANNOSE SYNTHASE1 mediates the biogenesis of isoprenyl-linked glycans and influences development, stress response, and ammonium hypersensitivity in Arabidopsis.
abstract: The most abundant posttranslational modification in nature is the attachment of preassembled high-mannose-type glycans, which determines the fate and localization of the modified protein and modulates the biological functions of glycosylphosphatidylinositol-anchored and N-glycosylated proteins. In eukaryotes, all mannose residues attached to glycoproteins from the luminal side of the endoplasmic reticulum (ER) derive from the polyprenyl monosaccharide carrier, dolichol P-mannose (Dol-P-Man), which is flipped across the ER membrane to the lumen. We show that in plants, Dol-P-Man is synthesized when Dol-P-Man synthase1 (DPMS1), the catalytic core, interacts with two binding proteins, DPMS2 and DPMS3, that may serve as membrane anchors for DPMS1 or provide catalytic assistance. This configuration is reminiscent of that observed in mammals but is distinct from the single DPMS protein catalyzing Dol-P-Man biosynthesis in bakers' yeast and protozoan parasites. Overexpression of DPMS1 in Arabidopsis thaliana results in disorganized stem morphology and vascular bundle arrangements, wrinkled seed coat, and constitutive ER stress response. Loss-of-function mutations and RNA interference-mediated reduction of DPMS1 expression in Arabidopsis also caused a wrinkled seed coat phenotype and most remarkably enhanced hypersensitivity to ammonium that was manifested by extensive chlorosis and a strong reduction of root growth. Collectively, these data reveal a previously unsuspected role of the prenyl-linked carrier pathway for plant development and physiology that may help integrate several aspects of candidate susceptibility genes to ammonium stress.
authors.affiliation: Institut de Biologie Moléculaire des Plantes du Centre National de la Recherche Scientifique, Université de Strasbourg, Strasbourg Cedex, France.
authors.forename: Nurul
authors.initials: N
authors.lastname: Jadid
authors.forename: Alexis Samba
authors.initials: AS
authors.lastname: Mialoundama
authors.forename: Dimitri
authors.initials: D
authors.lastname: Heintz
authors.forename: Daniel
authors.initials: D
authors.lastname: Ayoub
authors.forename: Mathieu
authors.initials: M
authors.lastname: Erhardt
authors.forename: Jérôme
authors.initials: J
authors.lastname: Mutterer
authors.forename: Denise
authors.initials: D
authors.lastname: Meyer
authors.forename: Abdelmalek
authors.initials: A
authors.lastname: Alioua
authors.forename: Alain
authors.initials: A
authors.lastname: Van Dorsselaer
authors.forename: Alain
authors.initials: A
authors.lastname: Rahier
authors.forename: Bilal
authors.initials: B
authors.lastname: Camara
authors.forename: Florence
authors.initials: F
authors.lastname: Bouvier
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D001205:Ascorbic Acid / Q000032:analysis / Q000378:metabolism; D004287:Dolichol Monophosphate Mannose / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000378:metabolism; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D006031:Glycosylation; D008364:Mannosyltransferases / Q000235:genetics / Q000378:metabolism*; D016254:Mutagenesis, Insertional; D010641:Phenotype; D018515:Plant Leaves / Q000187:drug effects / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D018547:Plant Stems / Q000187:drug effects / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D011134:Polysaccharides / Q000378:metabolism*; D060066:Protein Interaction Maps; D011499:Protein Processing, Post-Translational; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology*; D012639:Seeds / Q000187:drug effects / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D013312:Stress, Physiological
chemical_list: D029681:Arabidopsis Proteins; D011134:Polysaccharides; D000644:Quaternary Ammonium Compounds; D004287:Dolichol Monophosphate Mannose; D008364:Mannosyltransferases; C022755:dolichyl-phosphate beta-D-mannosyltransferase; D001205:Ascorbic Acid