title: Feruloylated and nonferuloylated arabino-oligosaccharides from sugar beet pectin selectively stimulate the growth of Bifidobacterium spp. in human fecal in vitro fermentations.
abstract: The side chains of the rhamnogalacturonan I fraction in sugar beet pectin are particularly rich in arabinan moieties, which may be substituted with feruloyl groups. In this work the arabinan-rich fraction resulting from sugar beet pulp based pectin production was separated by Amberlite XAD hydrophobic interaction and membrane separation into four fractions based on feruloyl substitution and arabino-oligosaccharide chain length: short-chain (DP 2-10) and long-chain (DP 7-14) feruloylated and nonferuloylated arabino-oligosaccharides, respectively. HPAEC, SEC, and MALDI-TOF/TOF analyses of the fractions confirmed the presence of singly and doubly substituted feruloylated arabino-oligosaccharides in the feruloyl-substituted fractions. In vitro microbial fermentation by human fecal samples (n = 6 healthy human volunteers) showed a selective stimulation of bifidobacteria by both the feruloylated and the nonferuloylated long-chain arabino-oligosaccharides to the same extent as the prebiotic fructo-oligosaccharides control. None of the fractions stimulated the growth of the potential pathogen Clostridium difficile in monocultures. This work provides a first report on the separation of potentially bioactive feruloylated arabino-oligosaccharides from sugar beet pulp and an initial indication of the potentially larger bifidogenic effect of relatively long-chain arabino-oligosaccharides as opposed to short-chain arabino-oligosaccharides.
authors.affiliation: Center for Bioprocess Engineering, Department of Chemical and Biochemical Engineering, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark.
authors.forename: Jesper
authors.initials: J
authors.lastname: Holck
authors.forename: Andrea
authors.initials: A
authors.lastname: Lorentzen
authors.forename: Louise K
authors.initials: LK
authors.lastname: Vigsnæs
authors.forename: Tine R
authors.initials: TR
authors.lastname: Licht
authors.forename: Jørn D
authors.initials: JD
authors.lastname: Mikkelsen
authors.forename: Anne S
authors.initials: AS
authors.lastname: Meyer
mesh_terms: D001089:Arabinose / Q000737:chemistry / Q000378:metabolism; D027461:Beta vulgaris / Q000737:chemistry*; D001644:Bifidobacterium / Q000254:growth & development* / Q000378:metabolism*; D005243:Feces / Q000382:microbiology*; D005285:Fermentation; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000382:microbiology*; D008954:Models, Biological; D015394:Molecular Structure; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D010368:Pectins / Q000737:chemistry* / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D056692:Prebiotics / Q000032:analysis
chemical_list: D009844:Oligosaccharides; D010936:Plant Extracts; D056692:Prebiotics; D010368:Pectins; D001089:Arabinose