title: Metabolic diversity among main microorganisms inside an arsenic-rich ecosystem revealed by meta- and proteo-genomics.
abstract: By their metabolic activities, microorganisms have a crucial role in the biogeochemical cycles of elements. The complete understanding of these processes requires, however, the deciphering of both the structure and the function, including synecologic interactions, of microbial communities. Using a metagenomic approach, we demonstrated here that an acid mine drainage highly contaminated with arsenic is dominated by seven bacterial strains whose genomes were reconstructed. Five of them represent yet uncultivated bacteria and include two strains belonging to a novel bacterial phylum present in some similar ecosystems, and which was named 'Candidatus Fodinabacter communificans.' Metaproteomic data unravelled several microbial capabilities expressed in situ, such as iron, sulfur and arsenic oxidation that are key mechanisms in biomineralization, or organic nutrient, amino acid and vitamin metabolism involved in synthrophic associations. A statistical analysis of genomic and proteomic data and reverse transcriptase-PCR experiments allowed us to build an integrated model of the metabolic interactions that may be of prime importance in the natural attenuation of such anthropized ecosystems.
authors.affiliation: Génétique Moléculaire, Génomique et Microbiologie, UMR7156 CNRS and UdS, Strasbourg, France. philippe.bertin@unistra.fr
authors.forename: Philippe N
authors.initials: PN
authors.lastname: Bertin
authors.forename: Audrey
authors.initials: A
authors.lastname: Heinrich-Salmeron
authors.forename: Eric
authors.initials: E
authors.lastname: Pelletier
authors.forename: Florence
authors.initials: F
authors.lastname: Goulhen-Chollet
authors.forename: Florence
authors.initials: F
authors.lastname: Arsène-Ploetze
authors.forename: Sébastien
authors.initials: S
authors.lastname: Gallien
authors.forename: Béatrice
authors.initials: B
authors.lastname: Lauga
authors.forename: Corinne
authors.initials: C
authors.lastname: Casiot
authors.forename: Alexandra
authors.initials: A
authors.lastname: Calteau
authors.forename: David
authors.initials: D
authors.lastname: Vallenet
authors.forename: Violaine
authors.initials: V
authors.lastname: Bonnefoy
authors.forename: Odile
authors.initials: O
authors.lastname: Bruneel
authors.forename: Béatrice
authors.initials: B
authors.lastname: Chane-Woon-Ming
authors.forename: Jessica
authors.initials: J
authors.lastname: Cleiss-Arnold
authors.forename: Robert
authors.initials: R
authors.lastname: Duran
authors.forename: Françoise
authors.initials: F
authors.lastname: Elbaz-Poulichet
authors.forename: Nuria
authors.initials: N
authors.lastname: Fonknechten
authors.forename: Ludovic
authors.initials: L
authors.lastname: Giloteaux
authors.forename: David
authors.initials: D
authors.lastname: Halter
authors.forename: Sandrine
authors.initials: S
authors.lastname: Koechler
authors.forename: Marie
authors.initials: M
authors.lastname: Marchal
authors.forename: Damien
authors.initials: D
authors.lastname: Mornico
authors.forename: Christine
authors.initials: C
authors.lastname: Schaeffer
authors.forename: Adam Alexander Thil
authors.initials: AA
authors.lastname: Smith
authors.forename: Alain
authors.initials: A
authors.lastname: Van Dorsselaer
authors.forename: Jean
authors.initials: J
authors.lastname: Weissenbach
authors.forename: Claudine
authors.initials: C
authors.lastname: Médigue
authors.forename: Denis
authors.initials: D
authors.lastname: Le Paslier
mesh_terms: D001151:Arsenic / Q000378:metabolism*; D001419:Bacteria / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D017753:Ecosystem; D007501:Iron / Q000378:metabolism; D056186:Metagenomics; D008906:Mining; D010802:Phylogeny; D040901:Proteomics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013455:Sulfur / Q000378:metabolism
chemical_list: D013455:Sulfur; D007501:Iron; D001151:Arsenic