title: Atorvastatin reduces lipopolysaccharide-induced expression of C-reactive protein in human lung epithelial cells.
abstract: Accumulating evidence suggests that statins possess anti-inflammatory properties and may decrease C-reactive protein (CRP) levels in plasma. However, no studies have as yet addressed whether or not statins regulate the expression of CRP in human lung epithelial cells (A549). In this study, we determined whether atorvastatin modulates the lipopolysaccharide (LPS)-induced expression of CRP in A549 cells. A549 cells were incubated in Dulbecco's modified Eagle's medium containing LPS in the absence or presence of various concentrations of atorvastatin. After incubation, the medium was collected and the amount of CRP was measured by an enzyme-linked immunosorbent assay. The cells were harvested and CRP messenger ribonucleic acid (mRNA) was analyzed by reverse transcription polymerase chain reaction. Incubation with LPS induced a significant time- and dose-dependent increase in CRP mRNA expression and CRP production in A549 cells, whereas atorvastatin significantly decreased LPS-induced CRP mRNA expression and CRP production in a dose-dependent manner. The present study revealed that A549 cells are capable of LPS-induced CRP expression, and that atorvastatin down-regulates the LPS-induced expression of CRP in cultured A549 cells. Our results suggest that statins ameliorate lung inflammation by regulating CRP production in human lung epithelial cells.
authors.affiliation: First Department of Respiratory Medicine, Kunming Medical College, Yunnan 650051, People's Repulic of China.
authors.forename: Xi-Qian
authors.initials: XQ
authors.lastname: Xing
authors.forename: Shu
authors.initials: S
authors.lastname: Duan
authors.forename: Xu-Wei
authors.initials: XW
authors.lastname: Wu
authors.forename: Ye
authors.initials: Y
authors.lastname: Gan
authors.forename: Shui-Ping
authors.initials: SP
authors.lastname: Zhao
authors.forename: Ping
authors.initials: P
authors.lastname: Chen
authors.forename: Shang-Jie
authors.initials: SJ
authors.lastname: Wu
mesh_terms: D000924:Anticholesteremic Agents / Q000494:pharmacology*; D000069059:Atorvastatin; D002097:C-Reactive Protein / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D015536:Down-Regulation; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D006538:Heptanoic Acids / Q000494:pharmacology*; D006801:Humans; D008070:Lipopolysaccharides / Q000633:toxicity*; D011758:Pyrroles / Q000494:pharmacology*
chemical_list: D000924:Anticholesteremic Agents; D006538:Heptanoic Acids; D008070:Lipopolysaccharides; D011758:Pyrroles; D002097:C-Reactive Protein; D000069059:Atorvastatin