title: Use of a Vero cell-based fluorescent assay to assess relative toxicities of Shiga toxin 2 subtypes from Escherichia coli.
abstract: Shiga toxin-producing Escherichia coli is a leading cause of human gastroenteritis from food and waterborne sources worldwide. Shiga toxins 1 and 2 are important virulence factors linked to severe human illness. In particular, Shiga toxin 2 is composed of a diverse and heterogeneous group of subtypes with differential cytotoxicities in mammalian cells. In this chapter, we describe the use of the Vero-d2EGFP fluorescent assay to examine the relative toxicities of Stx2 and Stx2 subtypes expressed by strains of Shiga toxin-producing E. coli.
authors.affiliation: United States Department of Agriculture/Agricultural Research Service, Produce Safety and Microbiology Research Unit, Western Regional Research Center, Albany, CA, USA. Beatriz.Quinones@ars.usda.gov
authors.forename: Beatriz
authors.initials: B
authors.lastname: Quiñones
authors.forename: Michelle S
authors.initials: MS
authors.lastname: Swimley
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D005506:Food Contamination / Q000032:analysis; D049452:Green Fluorescent Proteins / Q000096:biosynthesis; D008856:Microscopy, Fluorescence / Q000379:methods*; D022622:Shiga Toxin 1 / Q000032:analysis / Q000633:toxicity; D022641:Shiga Toxin 2 / Q000032:analysis* / Q000633:toxicity; D054323:Shiga-Toxigenic Escherichia coli / Q000302:isolation & purification* / Q000472:pathogenicity*; D018675:Toxicity Tests; D014709:Vero Cells; D037521:Virulence Factors / Q000032:analysis / Q000633:toxicity
chemical_list: D022622:Shiga Toxin 1; D022641:Shiga Toxin 2; D037521:Virulence Factors; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins