title: Isolation and characterization of microsatellite loci in the entomopathogenic nematode Heterorhabditis bacteriophora.
abstract: Twenty microsatellite loci were identified from genomic DNA enrichment and expressed sequence tags of entomopathogenic nematode Heterorhabditis bacteriophora. Eight loci were found to be polymorphic in a Northeast Ohio H. bacteriophora population. Levels of polymorphism were evaluated in 31-56 individuals and the number of alleles ranged from two to three. The values of observed and expected heterozygosities ranged from 0 to 0.536 and from 0.223 to 0.616, respectively. All eight loci showed heterozygote deficiencies, but three conformed to Hardy-Weinberg equilibrium at the subpopulation level. This is the first set of microsatellite markers in entomopathogenic nematodes.
authors.affiliation: Department of Entomology, The Ohio State University - OARDC, 1680 Madison Avenue, Wooster, OH 44691, USA.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Bai
authors.forename: Amr T M
authors.initials: AT
authors.lastname: Saeb
authors.forename: Andrew
authors.initials: A
authors.lastname: Michel
authors.forename: Parwinder S
authors.initials: PS
authors.lastname: Grewal