title: A comparative study of the typhidot (Dot-EIA) and Widal tests in blood culture positive cases of typhoid fever.
abstract: Seventy-six blood culture positive typhoid cases and forty-eight controls were studied. The typhidot test was positive in 74 (97.36%) cases, with a sensitivity, specificity and positive predictive value of 96%, 89.5%, and 95%, respectively, compared to the Widal test which was positive in 56 (73.68%) cases with a sensitivity, specificity, and positive predictive value of 72%, 87%, and 87%, respectively (P = 0.001). In the control group, seven (14.5%) cases tested positive for the Widal test and two (4.16%) for the typhidot (P = 0.001), yielding the sensitivity and specificity for the Widal test and the typhidot test of 63% and 83%, and 85% and 97%, respectively. We conclude that the Dot-EIA (enzyme immunoassay; typhidot) is a more sensitive and specific test which is easy to perform and more reliable compared to the Widal test and that it is useful in early therapy.
authors.affiliation: Department of Medicine, Muhammad Medical College Hospital, Mirpurkhas, Sindh, Pakistan. drhajikhan786@yahoo.com
authors.forename: Haji Khan
authors.initials: HK
authors.lastname: Khoharo
mesh_terms: D000293:Adolescent; D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood*; D001769:Blood / Q000382:microbiology*; D003470:Culture Media; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods*; D008297:Male; D010154:Pakistan; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic; D012485:Salmonella typhi / Q000276:immunology* / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods*; D014435:Typhoid Fever / Q000175:diagnosis* / Q000382:microbiology; D055815:Young Adult
chemical_list: D000907:Antibodies, Bacterial; D003470:Culture Media; D011933:Reagent Kits, Diagnostic