title: Metabolite profiles of human immunodeficiency virus infected CD4+ T cells and macrophages using LC-MS/MS analysis.
abstract: Human immunodeficiency virus type 1 (HIV-1) infects both activated CD4+ T cells and macrophages. We tested if liquid chromatography-tandem mass spectrometry (LC-MS/MS) technology can monitor metabolic alterations induced by HIV-1 in the infected cells. Here we monitored glucose uptake and conducted LC-MS/MS-based metabolomic analysis in HIV-1 infected primary human CD4+ T cells and a macrophage model system: differentiated U1 (HIV-1 producing) and differentiated U937 (control) cells. HIV-1 infected CD4+ T cells have higher glucose uptake and increases in several metabolite pool sizes, whereas HIV-1 producing macrophages had substantial reductions in glucose uptake and steady state glycolytic intermediates. This data suggests that the two HIV-1 target cell types exhibit very different metabolic outcomes during viral production. This study also validates the LC-MS/MS technology as an effective metabolomic approach to monitor various metabolic alterations made by HIV-1 infection.
authors.affiliation: Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester, NY, USA.
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Hollenbaugh
authors.forename: Joshua
authors.initials: J
authors.lastname: Munger
authors.forename: Baek
authors.initials: B
authors.lastname: Kim
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000737:chemistry* / Q000378:metabolism* / Q000821:virology; D002460:Cell Line; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006678:HIV / Q000502:physiology*; D015658:HIV Infections / Q000378:metabolism* / Q000821:virology; D006801:Humans; D008264:Macrophages / Q000737:chemistry* / Q000378:metabolism* / Q000821:virology; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014779:Virus Replication