title: The conversion of centrioles to centrosomes: essential coupling of duplication with segregation.
abstract: Centrioles are self-reproducing organelles that form the core structure of centrosomes or microtubule-organizing centers (MTOCs). However, whether duplication and MTOC organization reflect innate activities of centrioles or activities acquired conditionally is unclear. In this paper, we show that newly formed full-length centrioles had no inherent capacity to duplicate or to organize pericentriolar material (PCM) but acquired both after mitosis through a Plk1-dependent modification that occurred in early mitosis. Modified centrioles initiated PCM recruitment in G1 and segregated equally in mitosis through association with spindle poles. Conversely, unmodified centrioles segregated randomly unless passively tethered to modified centrioles. Strikingly, duplication occurred only in centrioles that were both modified and disengaged, whereas unmodified centrioles, engaged or not, were "infertile," indicating that engagement specifically blocks modified centrioles from reduplication. These two requirements, centriole modification and disengagement, fully exclude unlimited duplication in one cell cycle. We thus uncovered a Plk1-dependent mechanism whereby duplication and segregation are coupled to maintain centriole homeostasis.
authors.affiliation: Cell Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10065, USA.
authors.forename: Won-Jing
authors.initials: WJ
authors.lastname: Wang
authors.forename: Rajesh Kumar
authors.initials: RK
authors.lastname: Soni
authors.forename: Kunihiro
authors.initials: K
authors.lastname: Uryu
authors.forename: Meng-Fu Bryan
authors.initials: MF
authors.lastname: Tsou
mesh_terms: D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D002502:Centrioles / Q000502:physiology* / Q000648:ultrastructure; D018385:Centrosome / Q000502:physiology* / Q000648:ultrastructure; D004847:Epithelial Cells / Q000502:physiology* / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells; D006706:Homeostasis; D006801:Humans; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D018715:Microscopy, Video; D008938:Mitosis; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D055213:Retinal Pigment Epithelium / Q000166:cytology / Q000502:physiology* / Q000648:ultrastructure; D013997:Time Factors; D014162:Transfection; D012359:tRNA Methyltransferases; D000097869:Polo-Like Kinase 1
chemical_list: D018797:Cell Cycle Proteins; D017346:Protein Serine-Threonine Kinases; D011506:Proteins; D011518:Proto-Oncogene Proteins; D012359:tRNA Methyltransferases; D000097869:Polo-Like Kinase 1; C497202:SASS6 protein, human; C498548:TRMO protein, human