title: Jaw and long bone marrow derived osteoclasts differ in shape and their response to bone and dentin.
abstract: Increasing evidence suggests the existence of osteoclast diversity. Here we investigated whether precursors obtained from marrow of the mandibula or long bone could give rise to phenotypically different osteoclasts. Formation of multinucleated cells was assessed after culturing mouse marrow cells of the two bone types with macrophage colony stimulating factor (M-CSF) and receptor activator of NFκB ligand (RANKL) for up to 10days on plastic, bone or dentin. Two times more osteoclasts formed from long bone marrow cells on bone compared to dentin, whereas higher numbers of jaw osteoclasts formed on dentin. Resorption of dentin or bone was similar for osteoclasts formed from both types of precursors. In contrast to jaw marrow derived osteoclasts, long bone osteoclasts predominantly had a multi-compartmented shape, with at least two nuclei containing compartments per cell. Osteoclasts on bone contained two times more actin rings than osteoclasts on dentin, regardless of their precursor origin. However, the area per osteoclast covered by actin rings was similar (20%) for both substrates. This study suggests that marrow cells obtained from different bones give rise to different osteoclasts. The substrate on which the osteoclasts are generated plays a role in steering their formation rather than their resorption.
authors.affiliation: Department of Periodontology, University of Amsterdam, VU University Amsterdam, Research Institute MOVE, Gustav Mahlerlaan 3004, 1081 LA Amsterdam, The Netherlands.
authors.forename: Azin
authors.initials: A
authors.lastname: Azari
authors.forename: Ton
authors.initials: T
authors.lastname: Schoenmaker
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: de Souza Faloni
authors.forename: Vincent
authors.initials: V
authors.lastname: Everts
authors.forename: Teun J
authors.initials: TJ
authors.lastname: de Vries
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D001842:Bone and Bones; D002453:Cell Cycle; D002454:Cell Differentiation; D048430:Cell Shape; D003804:Dentin / Q000166:cytology*; D016173:Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D008297:Male; D008334:Mandible; D051379:Mice; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000378:metabolism; D010010:Osteoclasts / Q000166:cytology*; D053245:RANK Ligand / Q000378:metabolism
chemical_list: D016328:NF-kappa B; D053245:RANK Ligand; C505689:Tnfsf11 protein, mouse; D016173:Macrophage Colony-Stimulating Factor