title: Active role of small non-coding RNAs derived from SINE/B1 retrotransposon during early mouse development.
abstract: Small RNAs derived from repetitive sequences appear to play essential roles in mammalian gametogenesis and early development. In this study we focused on the short interspersed nucleotide element B1 (SINE/B1) small RNAs, which were zygotically expressed in pre-implantation mouse embryos; and we investigated whether the SINE/B1 small RNAs played an active role in gene silencing during early mouse development. The results indicated that the level of silencing activity involving the SINE/B1 small RNAs as mediators was significantly reduced in Dicer-knockdown mouse embryos. In addition, when the SINE/B1 small RNAs were mapped to a full-length SINE/B1 sequence, phase-distribution of the small RNAs appeared, suggesting possible enzymatic involvement. Therefore, our present study suggested that the zygotically expressed SINE/B1 small RNAs in pre-implantation mouse embryos contain active small RNAs, which were presumably processed by Dicer and involved in gene silencing.
authors.affiliation: Department of Molecular Genetics, National Institute of Neuroscience, NCNP, 4-1-1 Ogawahigashi, Kodaira, Tokyo 187-8502, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Ohnishi
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Totoki
authors.forename: Atsushi
authors.initials: A
authors.lastname: Toyoda
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Watanabe
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Katsushi
authors.initials: K
authors.lastname: Tokunaga
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Sakaki
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sasaki
authors.forename: Hirohiko
authors.initials: H
authors.lastname: Hohjoh
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000378:metabolism* / Q000502:physiology; D053487:DEAD-box RNA Helicases / Q000235:genetics; D018274:Electroporation; D047108:Embryonic Development / Q000235:genetics*; D005718:Gametogenesis / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D055785:Gene Knockdown Techniques; D059014:High-Throughput Nucleotide Sequencing; D051379:Mice; D058977:Molecular Sequence Annotation; D009841:Oligonucleotides / Q000235:genetics; D058727:RNA, Small Untranslated / Q000235:genetics / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D043244:Ribonuclease III / Q000235:genetics; D020086:Short Interspersed Nucleotide Elements / Q000235:genetics
chemical_list: D009841:Oligonucleotides; D058727:RNA, Small Untranslated; C509781:Dicer1 protein, mouse; D043244:Ribonuclease III; D053487:DEAD-box RNA Helicases