title: [Study of the antitumor activity of alveolar macrophages after transfected human INF-γ gene].
abstract: OBJECTIVE
  Alveolar macrophages (AMs) activated have the antitumor activity. The interferon-γ (INF-γ) is one of the stimulating factors. INF-γ can enhance the immune function of AMs in vitro. The aim of this study is to investigate the effect of human INF-γ gene on the antitumor activity of AMs when transfected into the alveolar macrophages (AMs) from the patients with lung cancer in vitro.
  
  
  METHODS
  AMs obtained by brochoalveolar lavage were separated and transfected by INF-γ gene. RT-PCR and ELISA were applied to determine whether the transfection was successful. The levels of tumor necrosis factor α (TNF-α), nitric oxide (NO) and interleukin-1 (IL-1) produced by AMs and the killing activity of AMs against L1210 cells was detected respectively.
  
  
  RESULTS
  Both RT-PCR and ELISA demonstrated that human INF-γ gene had been successfully transfected into AMs. When transfected by human INF-γ gene, the levels of TNF-α, NO and IL-1 produced by AMs from the patients with lung cancer and the killing activity of AMs against L1210 cells were significantly higher than those of the control groups.
  
  
  CONCLUSIONS
  Human INF-γ gene can enhance the antitumor activity of AMs when transfected into AMs from the patients with lung cancer.
authors.affiliation: Department of Respiratory Medicine, the Third Afiliated Hospital, Zhongshan University, Guangzhou 510630, China. zhoufenglizfl@126.com
authors.forename: Fengli
authors.initials: F
authors.lastname: Zhou
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Bi
authors.forename: Tiantuo
authors.initials: T
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Huang
mesh_terms: D002478:Cells, Cultured; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics* / Q000276:immunology*; D008175:Lung Neoplasms / Q000235:genetics* / Q000276:immunology; D016676:Macrophages, Alveolar / Q000276:immunology*; D014162:Transfection
chemical_list: D007371:Interferon-gamma