title: Characterization of microsatellite loci for the endangered cactus Echinocactus grusonii, and their cross-species utilization.
abstract: Echinocactus grusonii is common in trade but critically endangered in its natural habitat. With the ultimate aim of developing a certification scheme to aid in the conservation of this species, we have isolated E. grusonii microsatellites from a nonenriched library. Fifty-seven sequences contained a microsatellite array, of which 12 were polymorphic among 30 individuals from a single wild population. All 12 microsatellite primer pairs amplified product in one or more species in a screen of 27 other cactus species.
authors.affiliation: School of Biological Sciences, University of Reading, Whiteknights, Reading RG6 6AS, UK, CSIRO Sustainable Ecosystems, Atherton, Qld 4883, Australia, Escuela de Biologia, Fac. Ciencias Naturales, Universidad Autonoma de Queretaro, CP 76010 Queretaro, Mexico.
authors.forename: B D
authors.initials: BD
authors.lastname: Hardesty
authors.forename: S L
authors.initials: SL
authors.lastname: Hughes
authors.forename: V M
authors.initials: VM
authors.lastname: Rodriguez
authors.forename: J A
authors.initials: JA
authors.lastname: Hawkins