title: New sequence-tagged site molecular markers for identification of sex in Distichlis spicata.
abstract: Sex-linked molecular markers have become valuable tools for understanding sex ratio evolution and sex-specific physiology in pre-reproductive plants. To develop new accurate methods for sexing Distichlis spicata juveniles and nonflowering individuals, we converted a random amplified polymorphic DNA-polymerase chain reaction marker that co-segregated with the female phenotype into a set of sequence-tagged site markers. We tested the marker pair on known males and females from populations in Oregon and California. A single band was obtained for all female samples but never for males.
authors.affiliation: Department of Biology, Portland State University, PO Box 751, Portland, OR 97207-0751, USA.
authors.forename: Sarah M
authors.initials: SM
authors.lastname: Eppley
authors.forename: Robin
authors.initials: R
authors.lastname: O'Quinn
authors.forename: Anna L
authors.initials: AL
authors.lastname: Brown