title: Altered expression and secretion of colonic interleukin-6 in a stress-sensitive animal model of brain-gut axis dysfunction.
abstract: Interleukin-6 (IL-6) can activate gastrointestinal submucosal neurons, with associated implications for motility and secretory function. Patients with irritable bowel syndrome (IBS) have elevated levels of circulating IL-6. Colons from the Wistar Kyoto (WKY) rat model of IBS secrete more IL-6 (12.84 pg/ml) than control Sprague Dawley (SD) colons (5.55 pg/ml) and WKY secretions stimulated calcium responses in naïve submucosal neurons of greater amplitude. Recombinant IL-6 activated more submucosal neurons in WKY tissue preparations (p<0.05). These data demonstrate that WKY colonic supernatants activate submucosal neurons using an IL-6-dependent mechanism, thereby providing a link between gastrointestinal dysfunction and alterations in IL-6 levels.
authors.affiliation: Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland. d.omalley@ucc.ie
authors.forename: Dervla
authors.initials: D
authors.lastname: O'Malley
authors.forename: Timothy G
authors.initials: TG
authors.lastname: Dinan
authors.forename: John F
authors.initials: JF
authors.lastname: Cryan
mesh_terms: D000818:Animals; D001921:Brain / Q000276:immunology* / Q000378:metabolism; D003106:Colon / Q000276:immunology* / Q000378:metabolism; D004195:Disease Models, Animal; D041981:Gastrointestinal Tract / Q000276:immunology* / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis* / Q000378:metabolism; D043183:Irritable Bowel Syndrome / Q000276:immunology* / Q000378:metabolism* / Q000523:psychology; D008297:Male; D051381:Rats; D011921:Rats, Inbred WKY; D017207:Rats, Sprague-Dawley; D013045:Species Specificity; D013315:Stress, Psychological / Q000276:immunology* / Q000378:metabolism
chemical_list: D015850:Interleukin-6