title: Development and characterization of 30 polymorphic microsatellite markers for the Atlantic surfclam, Spisula solidissima (Dillwyn, 1817).
abstract: Thirty polymorphic microsatellite markers were developed for the Atlantic surfclam, Spisula solidissima, from an enriched library and characterized in 24 clams from a wild population. The number of alleles ranged from 3 to 16 per locus. The expected and observed heterozygosities ranged from 0.1942 to 0.9238 and 0.0833 to 0.875 respectively. Six loci showed significant (P < 0.05 after Bonferroni correction) deviation from Hardy-Weinberg equilibrium, probably because of the presence of null alleles. Three primer pairs amplified duplicated loci with two involving tandem mini-satellite repeats. Most of the microsatellite markers developed here should be useful for genetic studies in this species.
authors.affiliation: Ocean College, Hainan University, Key Laboratory of Tropic Biological Resources, MOE, Hainan Key Laboratory of Tropical Hydrobiological Technology, 58 Renmin Road, Haikou, Hainan 570228, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Aimin
authors.initials: A
authors.lastname: Wang
authors.forename: Ximing
authors.initials: X
authors.lastname: Guo