title: Printing of polymer microcapsules for enzyme immobilization on paper substrate.
abstract: Poly(ethyleneimine) (PEI) microcapsules containing laccase from Trametes hirsuta (ThL) and Trametes versicolor (TvL) were printed onto paper substrate by three different methods: screen printing, rod coating, and flexo printing. Microcapsules were fabricated via interfacial polycondensation of PEI with the cross-linker sebacoyl chloride, incorporated into an ink, and printed or coated on the paper substrate. The same ink components were used for three printing methods, and it was found that laccase microcapsules were compatible with the ink. Enzymatic activity of microencapsulated TvL was maintained constant in polymer-based ink for at least eight weeks. Thick layers with high enzymatic activity were obtained when laccase-containing microcapsules were screen printed on paper substrate. Flexo printed bioactive paper showed very low activity, since by using this printing method the paper surface was not fully covered by enzyme microcapsules. Finally, screen printing provided a bioactive paper with high water-resistance and the highest enzyme lifetime.
authors.affiliation: VTT Technical Research Center of Finland , 02044 VTT, Finland.
authors.forename: Anne
authors.initials: A
authors.lastname: Savolainen
authors.forename: Yufen
authors.initials: Y
authors.lastname: Zhang
authors.forename: Dominic
authors.initials: D
authors.lastname: Rochefort
authors.forename: Ulla
authors.initials: U
authors.lastname: Holopainen
authors.forename: Tomi
authors.initials: T
authors.lastname: Erho
authors.forename: Jouko
authors.initials: J
authors.lastname: Virtanen
authors.forename: Maria
authors.initials: M
authors.lastname: Smolander
mesh_terms: D001709:Biotechnology / Q000379:methods; D002214:Capsules / Q000737:chemistry / Q000378:metabolism; D004339:Drug Compounding / Q000379:methods; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D005656:Fungal Proteins / Q000737:chemistry / Q000378:metabolism*; D007281:Ink; D042845:Laccase / Q000737:chemistry / Q000378:metabolism*; D008031:Lignin / Q000378:metabolism; D010209:Paper; D011094:Polyethyleneimine / Q000737:chemistry* / Q000378:metabolism; D011327:Printing / Q000379:methods*; D055454:Trametes / Q000737:chemistry / Q000201:enzymology*
chemical_list: D002214:Capsules; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D011094:Polyethyleneimine; D008031:Lignin; D042845:Laccase