title: Development of urinary albumin immunosensor based on colloidal AuNP and PVA.
abstract: The development of immunosensors with high sensitivity and specificity in detecting the pathogenic or physiologically relevant molecules in the body, offers a powerful opportunity in early diagnosis and treatment of diseases. In this study, we developed a new competitive immunosensor with employing antibody (Ab) labeled AuNP (Ab-AuNP) and PVA modified screen-printed carbon electrode (SPCE) surface to detect the urine albumin. Field emission scanning electron microscopy (FE-SEM) of modified electrode showed a suitable and stable attachment between HSA antigen- mAb and AuNP. Cyclic voltammetric (CV) method demonstrated that modification process was well performed. Electrochemical measurements including differential pulse voltammetry (DPV) and square wave voltammetry (SWV) were employed for quantitative antigen detection. The electrochemical measurements performed with other proteins mixed with samples demonstrated a high specificity and selectivity for this biosensor in detecting the HSA. In optimal conditions, the immunosensor could detect HSA in a high linear range (from 2.5 to 200 μg/mL) with a low detection limit of 25 ng/mL. This new strategy could be improved and applied to detect the other antigen.
authors.affiliation: Endocrine and Metabolism Research Center, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran. omidfar@tums.ac.ir
authors.forename: Kobra
authors.initials: K
authors.lastname: Omidfar
authors.forename: Ahmad
authors.initials: A
authors.lastname: Dehdast
authors.forename: Hajar
authors.initials: H
authors.lastname: Zarei
authors.forename: Behnoush Khorsand
authors.initials: BK
authors.lastname: Sourkohi
authors.forename: Bagher
authors.initials: B
authors.lastname: Larijani
mesh_terms: D000418:Albumins / Q000032:analysis* / Q000276:immunology; D000419:Albuminuria / Q000175:diagnosis* / Q000652:urine*; D000818:Animals; D000911:Antibodies, Monoclonal; D015374:Biosensing Techniques / Q000379:methods* / Q000706:statistics & numerical data; D055664:Electrochemical Techniques; D017979:Gold Colloid; D006801:Humans; D053768:Metal Nanoparticles / Q000648:ultrastructure; D051379:Mice; D008855:Microscopy, Electron, Scanning; D011142:Polyvinyl Alcohol; D012709:Serum Albumin / Q000032:analysis
chemical_list: D000418:Albumins; D000911:Antibodies, Monoclonal; D017979:Gold Colloid; D012709:Serum Albumin; D011142:Polyvinyl Alcohol