title: Modulation of apoptosis by V protein mumps virus.
abstract: BACKGROUND
  The Urabe AM9 vaccine strain of mumps virus contains two variants of V protein: VWT (of HN-A1081 viral population) and VGly (of HN-G1081). The V protein is a promoting factor of viral replication by blocking the IFN antiviral pathway.
  
  
  RESULTS
  We studied the relationship between V protein variants and IFN-α2b-induced apoptosis. V proteins decrease activation of the extrinsic IFN-α2b-induced apoptotic pathway monitored by the caspase 8 activity, being the effect greater with the VWT protein. Both V proteins decrease the activity of caspase 9 of the intrinsic apoptotic pathway. In a system without IFN, the VWT and VGly proteins expression promotes activation of caspases 3 and 7. However, when the cellular system was stimulated with IFN-α, this activity decreased partially. TUNEL assay shows that for treatment with IFN-α and ibuprofen of cervical adenocarcinoma cells there is nuclear DNA fragmentation but the V protein expression reduces this process.
  
  
  CONCLUSIONS
  The reduction in the levels of caspases and DNA fragmentation, suggesting that V protein, particularly VWT protein of Urabe AM9 vaccine strain, modulates apoptosis. In addition, the VWT protein shows a protective role for cell proliferation in the presence of antiproliferative signals.
authors.affiliation: Laboratorio de Bioquímica y Biología Molecular, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Edif, 103H, CU-BUAP, San Manuel, CP 72550, Puebla, México. nora.rosas@correo.buap.mx
authors.forename: Nora H
authors.initials: NH
authors.lastname: Rosas-Murrieta
authors.forename: Gerardo
authors.initials: G
authors.lastname: Santos-López
authors.forename: Julio
authors.initials: J
authors.lastname: Reyes-Leyva
authors.forename: Francisca Sosa
authors.initials: FS
authors.lastname: Jurado
authors.forename: Irma
authors.initials: I
authors.lastname: Herrera-Camacho
mesh_terms: D017209:Apoptosis; D053148:Caspase 3 / Q000096:biosynthesis; D053179:Caspase 7 / Q000096:biosynthesis; D061945:Caspase Inhibitors; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000037:antagonists & inhibitors / Q000276:immunology; D009109:Mumps virus / Q000276:immunology* / Q000472:pathogenicity*; D011506:Proteins; D011994:Recombinant Proteins; D014764:Viral Proteins / Q000378:metabolism*; D037521:Virulence Factors / Q000378:metabolism*
chemical_list: D061945:Caspase Inhibitors; D000077190:Interferon alpha-2; D016898:Interferon-alpha; D011506:Proteins; D011994:Recombinant Proteins; D014764:Viral Proteins; D037521:Virulence Factors; D053148:Caspase 3; D053179:Caspase 7