title: Characterization of deoxynivalenol-induced anorexia using mouse bioassay.
abstract: A short-term mouse model was devised to investigate induction of food refusal by the common foodborne trichothecene deoxynivalenol (DON). DON dose-dependently induced anorexia within 2 h of exposure when administered either by intraperitoneal (ip.) injection or by oral gavage. The no observed adverse effect and lowest observed adverse effect levels in this assay were 0.5 and 1 mg/kg bw for ip. exposure and 1 and 2.5 mg/kg bw for oral exposure, respectively. DON's effects on food intake were transient, lasting up to 3h at 1 mg/kg bw and up to 6 h at 5 mg/kg bw. Interestingly, a dose-dependent orexigenic response was observed in the 14 h following the initial 2h food intake measurement. Toxin-treated mice exhibited partial resistance to feed refusal when exposed to DON subsequently after 2 d, but not after 7 d suggesting that this modest tolerance was reversible. The short-term mouse bioassay described here was useful in characterizing DON-induced anorexia and should be applicable to elucidating mechanisms underlying this adverse nutritional effect.
authors.affiliation: Department of Food Science and Human Nutrition, Michigan State University, East Lansing, MI 48824-1224, USA.
authors.forename: Brenna M
authors.initials: BM
authors.lastname: Flannery
authors.forename: Wenda
authors.initials: W
authors.lastname: Wu
authors.forename: James J
authors.initials: JJ
authors.lastname: Pestka
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000855:Anorexia / Q000139:chemically induced*; D001681:Biological Assay / Q000379:methods*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004435:Eating; D005260:Female; D016014:Linear Models; D051379:Mice; D019055:No-Observed-Adverse-Effect Level; D014255:Trichothecenes / Q000633:toxicity*
chemical_list: D014255:Trichothecenes; C007262:deoxynivalenol