title: Genotyping and drug resistance patterns of Mycobacterium tuberculosis strains in five provinces of China.
abstract: OBJECTIVE
  To evaluate the distribution of the Mycobacterium tuberculosis Beijing genotype and the association of the genotype with drug-resistant M. tuberculosis strains in five provinces in China.
  
  
  METHODS
  M. tuberculosis strains (n = 158) isolated from five provinces of China were subjected to insertion sequence 6110 restriction fragment length polymorphism (RFLP), spoligotyping and mycobacterial interspersed repetitive units (MIRU) analyses. The prevalence of the Beijing genotype strains in each province was determined and compared. The proportion method was used to test the drug susceptibility of all strains.
  
  
  RESULTS
  Of the 158 strains, 123 (77.8%) were identified as the Beijing genotype by RFLP and spoligotyping. Nearly all the strains (n = 152, 96.2%) were grouped into 14 shared spoligotypes. Six other spoligotypes were unique to China. The prevalence of the Beijing genotype was significantly higher in the interior than in coastal areas (P < 0.001, OR 5.4, 95%CI 2.3-12.7). Resistance to rifampicin (RMP) was associated with the Beijing strain (P = 0.05, OR 3.7, 95%CI 1.2-11.1).
  
  
  CONCLUSIONS
  The M. tuberculosis Beijing genotype varies in prevalence in different regions of China and is solely associated with RMP resistance.
authors.affiliation: Beijing Tuberculosis and Thoracic Tumour Research Institute, Beijing, China.
authors.forename: Y-L
authors.initials: YL
authors.lastname: Guo
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.forename: S-M
authors.initials: SM
authors.lastname: Wang
authors.forename: C-Y
authors.initials: CY
authors.lastname: Li
authors.forename: G-L
authors.initials: GL
authors.lastname: Jiang
authors.forename: G-L
authors.initials: GL
authors.lastname: Shi
authors.forename: C-X
authors.initials: CX
authors.lastname: Song
mesh_terms: D000328:Adult; D000904:Antibiotics, Antitubercular / Q000494:pharmacology; D000995:Antitubercular Agents / Q000494:pharmacology*; D002681:China / Q000453:epidemiology; D016172:DNA Fingerprinting; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D005260:Female; D005838:Genotype; D005843:Geography; D006801:Humans; D020071:Interspersed Repetitive Sequences; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D012293:Rifampin / Q000494:pharmacology; D014376:Tuberculosis / Q000188:drug therapy / Q000453:epidemiology* / Q000382:microbiology*; D055815:Young Adult
chemical_list: D000904:Antibiotics, Antitubercular; D000995:Antitubercular Agents; D012293:Rifampin