title: The vimentin-tubulin binding site peptide (Vim-TBS.58-81) crosses the plasma membrane and enters the nuclei of human glioma cells.
abstract: Cell-penetrating peptides (CPPs) can translocate through the plasma membrane and localize in different cell compartments providing a promising delivery system for peptides, proteins, nucleic acids, and other products. Here we describe features of a novel cell-penetrating peptide derived from the intermediate filament protein vimentin, called Vim-TBS.58-81. We show that it enters cells from a glioblastoma line via endocytosis where it distributes throughout the cytoplasm and nucleus. Moreover, when coupled to the pro-apoptogenic peptide P10, it localizes to the nucleus inhibiting cell proliferation. Thus, the Vim-TBS.58-81 peptide represents an effective vector for delivery of peptides and potentially a broad range of cargos to the nucleus.
authors.affiliation: Laboratoire Neurobiologie & Transgenese, UPRES-EA3143, Université d'Angers, Bâtiment IBS-IRIS, Centre Hospitalier Universitaire, 49033 Angers, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Balzeau
authors.forename: Alan
authors.initials: A
authors.lastname: Peterson
authors.forename: Joel
authors.initials: J
authors.lastname: Eyer
mesh_terms: D000255:Adenosine Triphosphate / Q000037:antagonists & inhibitors / Q000378:metabolism; D021941:Caveolae / Q000502:physiology; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism*; D049109:Cell Proliferation / Q000187:drug effects; D057846:Cell-Penetrating Peptides / Q000378:metabolism* / Q000494:pharmacology; D002772:Cholera Toxin / Q000378:metabolism; D018844:Cyclin-Dependent Kinases / Q000037:antagonists & inhibitors; D003593:Cytoplasm / Q000378:metabolism; D003847:Deoxyglucose / Q000494:pharmacology; D004705:Endocytosis / Q000187:drug effects / Q000502:physiology; D005909:Glioblastoma; D006801:Humans; D008856:Microscopy, Fluorescence; D016900:Neurofilament Proteins / Q000737:chemistry; D010446:Peptide Fragments / Q000378:metabolism* / Q000494:pharmacology; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D054730:Protein Interaction Domains and Motifs / Q000502:physiology*; D019810:Sodium Azide / Q000494:pharmacology; D013696:Temperature; D014168:Transferrin / Q000378:metabolism; D014404:Tubulin / Q000378:metabolism*; D014746:Vimentin / Q000737:chemistry*; D054322:tat Gene Products, Human Immunodeficiency Virus / Q000737:chemistry
chemical_list: D057846:Cell-Penetrating Peptides; D016900:Neurofilament Proteins; D010446:Peptide Fragments; D018809:Proliferating Cell Nuclear Antigen; D014168:Transferrin; D014404:Tubulin; D014746:Vimentin; C044882:neurofilament protein L; D054322:tat Gene Products, Human Immunodeficiency Virus; D000255:Adenosine Triphosphate; D002772:Cholera Toxin; D019810:Sodium Azide; D003847:Deoxyglucose; D018844:Cyclin-Dependent Kinases