title: In vitro screening for antihyperlipidemic activities in foodstuffs by evaluating lipoprotein profiles secreted from human hepatoma cells.
abstract: We screened the antihyperlipidemic effects of seven edible plants by evaluation of the triglyceride (TG) and cholesterol profiles secreted from HepG2 cells. We found that the water- and ethanol-extracts of Brasenia schreberi at 100 μg/ml exhibited strong inhibitory activities against TG and cholesterol secretions from HepG2 cells stimulated with sodium oleate. Real-time RT-PCR analysis demonstrated that ethanol extract of B. schreberi (BSET) attenuated the expression of the sterol regulatory element binding protein-1c and -2, fatty acid synthase and HMG CoA synthase-1 genes, which are involved in lipid synthesis in hepatocyte/hepatoma cells. Furthermore, we studied the action of BSET on adipose tissue accumulation and serum parameters in mice fed a high-fat diet (HFD). BSET suppressed mesenteric and epididymal adipose tissue accumulation and normalized serum TG and glucose, but not cholesterol levels in HFD-fed mice.
authors.forename: Jyunichiro
authors.initials: J
authors.lastname: Takahashi
authors.forename: Gen
authors.initials: G
authors.lastname: Toshima
authors.forename: Yukie
authors.initials: Y
authors.lastname: Matsumoto
authors.forename: Fumiko
authors.initials: F
authors.lastname: Kimura
authors.forename: Takanobu
authors.initials: T
authors.lastname: Kiuchi
authors.forename: Kentaro
authors.initials: K
authors.lastname: Hamada
authors.forename: Keishi
authors.initials: K
authors.lastname: Hata
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular; D002784:Cholesterol / Q000378:metabolism; D056945:Hep G2 Cells; D006801:Humans; D000960:Hypolipidemic Agents / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D050356:Lipid Metabolism / Q000187:drug effects; D008074:Lipoproteins / Q000378:metabolism*; D008113:Liver Neoplasms / Q000188:drug therapy / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014280:Triglycerides / Q000378:metabolism
chemical_list: D000960:Hypolipidemic Agents; D008074:Lipoproteins; D010936:Plant Extracts; D014280:Triglycerides; D002784:Cholesterol