title: Cytochrome P450 1A1 expression in cetacean skin biopsies from the Indian Ocean.
abstract: The study describes cytochrome P450 1A1 (CYPA1) expression in the skin of different cetacean species (Megaptera novaeangliae, n=15; Stenella attenuata, n=7 and Stenella longirostris, n=24) from the Mozambique Channel island of Mayotte. Immunohistochemical examination was performed with a monoclonal antibody against scup cytochrome CYPA1. The sex was determined using a molecular approach consisting in the genotyping sex-specific genes. CYPA1 was detected at the junction between epidermis and blubber on dolphins only, mostly in the endothelial cells. Similar observation was obtained in the dermis of one M. novaeangliae. Immunohistochemical slides were scored to evaluate the expression of the CYPA1 and a higher expression was observed in S. longirostris, suggesting a higher exposure to pollutants for this species. The difference of expression between sexes was not significant.
authors.affiliation: Dept. of Pathology, Veterinary College, Sart Tilman B43, University of Liege, Belgium. t.jauniaux@ulg.ac.be
authors.forename: Thierry
authors.initials: T
authors.lastname: Jauniaux
authors.forename: Frédéric
authors.initials: F
authors.lastname: Farnir
authors.forename: Michaël
authors.initials: M
authors.lastname: Fontaine
authors.forename: Jeremy
authors.initials: J
authors.lastname: Kiszka
authors.forename: Michael
authors.initials: M
authors.lastname: Sarlet
authors.forename: Freddy
authors.initials: F
authors.lastname: Coignoul
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism*; D004781:Environmental Exposure / Q000032:analysis / Q000706:statistics & numerical data; D005260:Female; D050640:Humpback Whale / Q000378:metabolism*; D019156:Indian Ocean; D008297:Male; D012867:Skin / Q000378:metabolism*; D050706:Stenella / Q000378:metabolism*; D014873:Water Pollutants / Q000633:toxicity; D014876:Water Pollution / Q000706:statistics & numerical data
chemical_list: D015415:Biomarkers; D014873:Water Pollutants; D019363:Cytochrome P-450 CYP1A1