title: Thermosyntropha tengcongensis sp. nov., a thermophilic bacterium that degrades long-chain fatty acids syntrophically.
abstract: A novel anaerobic, thermophilic, syntrophic, fatty-acid-oxidizing bacterium, strain L-60T, was isolated from a Chinese hot spring. Cells of the strain were non-motile, non-spore-forming, slightly curved rods. Growth occurred between 55 and 70 °C (optimum about 60 °C) and at pH 7.0-9.3 (optimum about pH 8.2). Crotonate was the only tested carbon source that supported the growth of the strain in pure culture. In co-culture with the thermophilic, hydrogenotrophic Methanothermobacter thermautotrophicus DSM 1053T, the isolate could oxidize saturated straight-chain fatty acids with 4-18 carbon atoms, and also unsaturated fatty acids such as oleate, syntrophically. The strain was unable to utilize sulfate, sulfite, thiosulfate, nitrate, fumarate or Fe(III) as electron acceptors. The major cellular fatty acids were C14:0 (35.0 %), C16:0 (20.3 %) and iso-C17:1 I/anteiso-C17:1 B (30.9 %). The genomic DNA G+C content was 40.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain was affiliated to the family Syntrophomonadaceae and was most closely related to Thermosyntropha lipolytica DSM 11003T (96.7 % similarity). On the basis of phylogenetic and phenotypic evidence, it is proposed that strain L-60T represents a novel species, for which the name Thermosyntropha tengcongensis sp. nov. is proposed. The type strain is L-60T (=CGMCC 1.5161T=JCM 17260T).
authors.affiliation: Graduate University, Chinese Academy of Sciences, Beijing 100049, PR China.|State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Fan
authors.initials: F
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Xiuzhu
authors.initials: X
authors.lastname: Dong
mesh_terms: D001421:Bacteria, Anaerobic / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D018920:Coculture Techniques; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism; D006094:Gram-Positive Bacteria / Q000235:genetics / Q000378:metabolism; D045482:Hot Springs / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S