title: A novel approach to the rapid determination of amoxicillin in human plasma by solid phase microextraction and liquid chromatography.
abstract: A new approach to the rapid determination of amoxicillin (AMO) in human plasma followed by solid phase microextraction (SPME) fiber coatings based on conducting polymers (polypyrrole and polythiophene) and high performance liquid chromatography (HPLC) has been described. The porous structures of the electrochemically deposited polymer coatings have been characterized by scanning electron microscopy (SEM). The experimental parameters relating to the extraction efficiency of the SPME fibers such as pH, extraction time and desorption conditions (solvents, time) were studied and selected. The SPME/HPLC-UV method was linear over a working range of 1-50 μg ml(-1). The inter-day accuracy (expressed as coefficients of variations, CVs) was less than 15% and precision (expressed as the relative standard deviations, RSDs) with percentage values was less than 5.9%. Amoxicillin was found to be stable in the human plasma at room temperature (20 °C) within 8 hours. The developed method was successfully applied to the analysis of real human plasma samples. The limit of detection and limit of quantification for amoxicillin in plasma were 1.21 μg ml(-1) and 3.48 μg ml(-1), respectively.
authors.affiliation: Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Torun, Poland. bbusz@chem.uni.torun.pl
authors.forename: Boguslaw
authors.initials: B
authors.lastname: Buszewski
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Szultka
authors.forename: Pawel
authors.initials: P
authors.lastname: Olszowy
authors.forename: Szymon
authors.initials: S
authors.lastname: Bocian
authors.forename: Tomasz
authors.initials: T
authors.lastname: Ligor
mesh_terms: D000658:Amoxicillin / Q000097:blood* / Q000302:isolation & purification; D000900:Anti-Bacterial Agents / Q000097:blood* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D011108:Polymers / Q000737:chemistry; D011758:Pyrroles / Q000737:chemistry; D052617:Solid Phase Microextraction / Q000379:methods*; D013876:Thiophenes / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D011108:Polymers; D011758:Pyrroles; D013876:Thiophenes; C066730:polythiophene; C067635:polypyrrole; D000658:Amoxicillin