title: Fatty acid profile during the differentiation and infection with Mycobacterium tuberculosis of mononuclear phagocytes of patients with TB and healthy individuals.
abstract: The blockade of sPLA-2, as well as the removal of calcium during the infection with Mycobacterium tuberculosis, prevents necrosis in mononuclear phagocytes. In addition, previous evidence indicates that the necrosis is modulated by cytokines and may condition the inflammatory environment. The production of cytokines and chemokines in response to infection with M. tuberculosis, fatty acid profile and the lactate dehydrogenase activity in mononuclear phagocytes from tuberculosis patients and healthy controls were interrelated using a principal component analysis in order to establish whether there was an association between the induction and effector stages of necrosis with the production of cytokines and chemokines. Differentiation increased the ratio of saturated/unsaturated fatty acids. The oleate and palmitate correlated with differentiation, laureate, arachidonate and linolenate with infection and necrosis correlates with the production of IL-10. Monocytes from tuberculosis patients seem to be lees differentiated ex vivo.
authors.affiliation: Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia. mrojaslop@hotmail.com
authors.forename: Maria Elena
authors.initials: ME
authors.lastname: Ramírez-Agudelo
authors.forename: Ana Cecilia
authors.initials: AC
authors.lastname: Caro
authors.forename: Carlos Alberto Peláez
authors.initials: CA
authors.lastname: Jaramillo
authors.forename: Mauricio
authors.initials: M
authors.lastname: Rojas
mesh_terms: D016022:Case-Control Studies; D002454:Cell Differentiation; D018925:Chemokines / Q000096:biosynthesis; D016207:Cytokines / Q000096:biosynthesis; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism*; D054884:Host-Pathogen Interactions / Q000276:immunology / Q000502:physiology; D006801:Humans; D066298:In Vitro Techniques; D016753:Interleukin-10 / Q000096:biosynthesis; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D055442:Metabolome; D009169:Mycobacterium tuberculosis / Q000472:pathogenicity*; D009336:Necrosis; D010586:Phagocytes / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology* / Q000473:pathology; D014397:Tuberculosis, Pulmonary / Q000378:metabolism* / Q000382:microbiology* / Q000473:pathology
chemical_list: D018925:Chemokines; D016207:Cytokines; D005227:Fatty Acids; C508609:IL10 protein, human; D016753:Interleukin-10; D007770:L-Lactate Dehydrogenase