title: UVB-induced apoptosis and DNA damaging potential of chrysene via reactive oxygen species in human keratinocytes.
abstract: Chrysene is one of the basic polycyclic aromatic hydrocarbon (PAH) which is toxic environmental pollutant and consistently exposed to sunlight. However, little information is available on its photogenotoxicity. The objective of the present study was to analyze the effects of chrysene, under environmental intensity of UVB (0.6mW/cm(2)) in human skin epidermal cell line (HaCaT). Kinetic of chrysene showed that the highest intracellular uptake of chrysene occurred after 24h of incubation. The intracellular reactive oxygen species (ROS) was increased in a concentration dependent manner in chrysene treated cells under UVB irradiation. It was observed that UVB-irradiated chrysene induced apoptosis through activation of caspases-3 and phosphatidylserine translocation. Glutathione reduced (GSH) and catalase activity were decreased while apoptosis and DNA damage were induced significantly (P>0.01) as concentration of chrysene increased. Thus our results suggest that chrysene may be phototoxic as well as photogenotoxic under UVB irradiation.
authors.affiliation: Photobiology Division, Indian Institute of Toxicology Research (CSIR), Post Box No. 80, M.G. Marg, Lucknow 226001, Uttar Pradesh, India.
authors.forename: Daoud
authors.initials: D
authors.lastname: Ali
authors.forename: A
authors.initials: A
authors.lastname: Verma
authors.forename: F
authors.initials: F
authors.lastname: Mujtaba
authors.forename: A
authors.initials: A
authors.lastname: Dwivedi
authors.forename: R K
authors.initials: RK
authors.lastname: Hans
authors.forename: R S
authors.initials: RS
authors.lastname: Ray
mesh_terms: D017304:Annexin A5 / Q000032:analysis; D017209:Apoptosis / Q000187:drug effects* / Q000528:radiation effects; D053148:Caspase 3 / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D002911:Chrysenes / Q000633:toxicity*; D004249:DNA Damage; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects; D010718:Phosphatidylserines / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D014466:Ultraviolet Rays
chemical_list: D017304:Annexin A5; D002911:Chrysenes; D010718:Phosphatidylserines; D017382:Reactive Oxygen Species; C031180:chrysene; D002374:Catalase; D053148:Caspase 3; D005978:Glutathione