title: Potential role of the amelogenin N-terminus in the regulation of calcium phosphate formation in vitro.
abstract: N-terminal and C-terminal (CT) domains of amelogenin have been shown to be essential for proper enamel formation. Recent studies have also suggested that although the C-terminus plays an apparent role in protein-mineral interactions, other amelogenin structural domains are involved. The objective was to explore the role of the amelogenin N-terminus in the regulation of calcium phosphate formation in vitro. Spontaneous mineralization studies were carried out using the phosphorylated (+P) and nonphosphorylated (-P) N-terminus of the leucine-rich amelogenin peptide (LRAP) that lacks the hydrophilic CT domain. Mineralization progress was monitored via changes in solution pH. Mineral phases formed were characterized using TEM, selected area electron diffraction, and FT-IR. In controls, amorphous calcium phosphate was initially formed and subsequently transformed to randomly oriented hydroxyapatite (HA) plate-like crystals. In contrast to the control, LRAP(+P)-CT stabilized ACP formation for >1 day, while LRAP(-P)-CT accelerated the transformation of ACP to HA but had little effect on crystal shape or orientation. In conclusion, the N-terminal domain found in LRAP, as in amelogenins, appears to have the capacity to interact with forming calcium phosphate mineral phases. Results suggest that the N-terminal domain of amelogenin may play a direct role in early stages of enamel formation.
authors.affiliation: Department of Biomineralization, The Forsyth Institute, Cambridge, Mass., USA.
authors.forename: E
authors.initials: E
authors.lastname: Le Norcy
authors.forename: S-Y
authors.initials: SY
authors.lastname: Kwak
authors.forename: F B
authors.initials: FB
authors.lastname: Wiedemann-Bidlack
authors.forename: E
authors.initials: E
authors.lastname: Beniash
authors.forename: Y
authors.initials: Y
authors.lastname: Yamakoshi
authors.forename: J P
authors.initials: JP
authors.lastname: Simmer
authors.forename: H C
authors.initials: HC
authors.lastname: Margolis
mesh_terms: D053523:Amelogenin / Q000737:chemistry* / Q000378:metabolism* / Q000648:ultrastructure; D000595:Amino Acid Sequence; D000818:Animals; D002113:Calcification, Physiologic / Q000502:physiology; D002130:Calcium Phosphates / Q000378:metabolism*; D003746:Dental Enamel Proteins / Q000737:chemistry / Q000648:ultrastructure; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D017550:Spectroscopy, Fourier Transform Infrared; D013329:Structure-Activity Relationship; D034421:Sus scrofa; D013997:Time Factors
chemical_list: D053523:Amelogenin; D002130:Calcium Phosphates; D003746:Dental Enamel Proteins; C067432:leucine-rich amelogenin peptide