title: [Mechanism of DADS in the bystander effect of HSV-tk/GCV suicide gene therapy system in lens epithelial cells].
abstract: OBJECTIVE
  To investigate the mechanism and effect of diallyl disulfide (DADS) on the bystander effect of herpes simplex virus kinase/ganciclovir (HSV-tk/GCV) suicide gene therapy system which was mediated by recombinant adeno-associated virus (rAAV) in lens epithelial cells.
  
  
  METHODS
  Immunohistochemistry method was used to determine the distribution of connexin 43 (Cx43) protein in cultured rabbit lens epithelial cells and rabbit lens epithelial cells transfected by HSV-tk suicide gene. Cx43 protein was measured and analyzed in cultured rabbit lens epithelial cells and rabbit lens epithelial cells transfected by HSV-tk suicide gene that was induced by various DADS. Cell survival was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method.
  
  
  RESULTS
  DADS increased the Cx43 protein expression in cultured rabbit lens epithelial cells and rabbit lens epithelial cells transfected by HSV-tk suicide gene, and especially in 50 μmol/L DADS. After combining with DADS, the bystander effect was significantly improved (P<0.05).
  
  
  CONCLUSIONS
  DADS can elevate the expression of Cx43 protein and enhance the bystander effect of HSV-tk/GCV suicide gene therapy system.
authors.affiliation: Department of Ophthalmology, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Zhongqing
authors.initials: Z
authors.lastname: Li
authors.forename: Qian
authors.initials: Q
authors.lastname: Tan
authors.forename: Zhixiang
authors.initials: Z
authors.lastname: Ding
authors.forename: Dan
authors.initials: D
authors.lastname: Liu
mesh_terms: D000256:Adenoviridae / Q000235:genetics / Q000378:metabolism; D000498:Allyl Compounds / Q000494:pharmacology*; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology; D024201:Bystander Effect / Q000187:drug effects*; D002478:Cells, Cultured; D018031:Connexin 43 / Q000378:metabolism; D004220:Disulfides / Q000494:pharmacology*; D004847:Epithelial Cells / Q000378:metabolism; D015774:Ganciclovir / Q000494:pharmacology; D041722:Genes, Transgenic, Suicide; D007908:Lens, Crystalline / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010938:Plant Oils; D011817:Rabbits; D018139:Simplexvirus / Q000201:enzymology*; D013937:Thymidine Kinase / Q000235:genetics* / Q000378:metabolism
chemical_list: D000498:Allyl Compounds; D000998:Antiviral Agents; D018031:Connexin 43; D004220:Disulfides; D010938:Plant Oils; C028009:diallyl disulfide; D013937:Thymidine Kinase; D015774:Ganciclovir