title: A novel homogenous detection method based on the self-assembled DNAzyme labeled DNA probes with SWNT conjugates and its application in detecting pathogen.
abstract: In this paper, a novel and cost-effective homogeneous detection method was constructed for the detection of genomic DNA and Staphylococcus aureus (S. aureus), based on the noncovalent assembly of DNAzyme-labeled detection probe and single-walled carbon nanotubes (SWNTs). When the target genomic DNA and hemin was existed in the detection solution, the detection probe wrapped on the SWNTs by π-stacking interactions would keep away from SWNTs and form a DNAzyme-self-assembly construction. This DNAzyme construction could catalyze 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS²⁻) and generate a colored product which could lead to the absorbance changes. Hence, according to its catalyzed capacity, the DNAzyme construction could amplify the detection signal. The concentration of target DNA could be quantified by exploiting their optical absorption changes at 414 nm and the concentration limit of detection of the method was 30 nM. And this detection method detected S. aureus quantitatively. In addition, this work proved that the method obtain higher detection sensitivity compared with the method without SWNTs because of the protection profile of SWNTs towards the detection probe.
authors.affiliation: The Co-construction Laboratory of Microbial Molecular Biology of Province Department and Ministry of Science and Technology, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, People's Republic of China.
authors.forename: Xinghua
authors.initials: X
authors.lastname: Ding
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.forename: Le
authors.initials: L
authors.lastname: Deng
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Peng
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
authors.forename: Dan
authors.initials: D
authors.lastname: Wang
mesh_terms: D001431:Bacteriological Techniques; D052160:Benzothiazoles; D015374:Biosensing Techniques / Q000379:methods* / Q000706:statistics & numerical data; D015342:DNA Probes / Q000737:chemistry / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics*; D021881:DNA, Catalytic; D015336:Molecular Probe Techniques; D037742:Nanotubes, Carbon; D013211:Staphylococcus aureus / Q000235:genetics* / Q000302:isolation & purification*; D013451:Sulfonic Acids; D013844:Thiazoles
chemical_list: D052160:Benzothiazoles; D015342:DNA Probes; D004269:DNA, Bacterial; D021881:DNA, Catalytic; D037742:Nanotubes, Carbon; D013451:Sulfonic Acids; D013844:Thiazoles; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid