title: Electrochemistry of cytochrome c1, cytochrome c552, and CuA from the respiratory chain of Thermus thermophilus immobilized on gold nanoparticles.
abstract: The electrochemical behavior of three proteins fragments from the respiratory chain of the extremophilic bacterium Thermus thermophilus , namely, cytochrome c(1) (Cyt-c(1)), cytochrome c(552) (Cyt-c(552)), and Cu(A), immobilized on three-dimensional gold nanoparticles electrodes was investigated by cyclic voltammetry. The gold nanoparticles were modified by either dithiobissuccinimidyl propionate (DTSP) or a mixed self-assembled monolayer of 6-mercaptohexan-1-ol and hexanethiol, depending on the surface of the protein. High surface coverages with enzymes and good electron transfer rates were achieved in the case of Cyt-c(1) immobilized on DTSP-modified gold nanoparticles and Cyt-c(552) or Cu(A) immobilized on mixed SAMs-modified gold nanoparticles. Interestingly, high surface coverages with Cu(A) were also observed on DTSP-modified gold nanoparticles, but a slower electron transfer rate was determined in this case. The gold nanoparticle/protein assemblies were characterized by surface-enhanced IR spectroscopy and transmission electron microscopy.
authors.affiliation: Laboratoire de Spectroscopie Vibrationnelle et Electrochimie des Biomolécules (Institut de Chimie, UdS), Strasbourg, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Meyer
authors.forename: Julien
authors.initials: J
authors.lastname: Gross
authors.forename: Christian
authors.initials: C
authors.lastname: Blanck
authors.forename: Marc
authors.initials: M
authors.lastname: Schmutz
authors.forename: Bernd
authors.initials: B
authors.lastname: Ludwig
authors.forename: Petra
authors.initials: P
authors.lastname: Hellwig
authors.forename: Frederic
authors.initials: F
authors.lastname: Melin
mesh_terms: D003574:Cytochrome c Group / Q000737:chemistry* / Q000378:metabolism; D003575:Cytochromes c1 / Q000737:chemistry* / Q000378:metabolism; D004563:Electrochemistry; D004566:Electrodes; D003576:Electron Transport Complex IV / Q000737:chemistry* / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D008958:Models, Molecular; D010316:Particle Size; D013499:Surface Properties; D016963:Thermus thermophilus / Q000737:chemistry* / Q000378:metabolism
chemical_list: D003574:Cytochrome c Group; D006046:Gold; D003575:Cytochromes c1; C022302:cytochrome C-552; D003576:Electron Transport Complex IV