title: Imbricatolic acid from Juniperus communis L. prevents cell cycle progression in CaLu-6 cells.
abstract: Imbricatolic acid was isolated from the methanolic extract of the fresh ripe berries of Juniperus communis (Cupressaceae) together with sixteen known compounds and a new dihydrobenzofuran lignan glycoside named juniperoside A. Their structures were determined by spectroscopic methods and by comparison with the spectral data reported in literature. Imbricatolic acid was evaluated for its ability to prevent cell cycle progression in p53-null CaLu-6 cells. This compound induces the upregulation of cyclin-dependent kinase inhibitors and their accumulation in the G1 phase of the cell cycle, as well as the degradation of cyclins A, D1, and E1. Furthermore, no significant imbricatolic acid-induced apoptosis was observed. Therefore, this plant-derived compound may play a role in the control of cell cycle.
authors.affiliation: Dipartimento di Chimica delle Sostanze Naturali, Università degli Studi di Napoli Federico II, Napoli, Italy.
authors.forename: Simona
authors.initials: S
authors.lastname: De Marino
authors.forename: Fabio
authors.initials: F
authors.lastname: Cattaneo
authors.forename: Carmen
authors.initials: C
authors.lastname: Festa
authors.forename: Franco
authors.initials: F
authors.lastname: Zollo
authors.forename: Annalisa
authors.initials: A
authors.lastname: Iaccio
authors.forename: Rosario
authors.initials: R
authors.lastname: Ammendola
authors.forename: Filomena
authors.initials: F
authors.lastname: Incollingo
authors.forename: Maria
authors.initials: M
authors.lastname: Iorizzi
mesh_terms: D000818:Animals; D000906:Antibodies; D045744:Cell Line, Tumor; D002470:Cell Survival; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000378:metabolism; D018844:Cyclin-Dependent Kinases / Q000378:metabolism; D016213:Cyclins / Q000378:metabolism; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D005638:Fruit / Q000737:chemistry; D016193:G1 Phase / Q000187:drug effects*; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification*; D006801:Humans; D007558:Italy; D018653:Juniperus / Q000737:chemistry*; D017705:Lignans / Q000737:chemistry / Q000302:isolation & purification*; D051379:Mice; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification*; D010946:Plants, Medicinal / Q000737:chemistry; D011817:Rabbits; D021241:Spectrometry, Mass, Electrospray Ionization; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013997:Time Factors; D016159:Tumor Suppressor Protein p53 / Q000235:genetics
chemical_list: D000906:Antibodies; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016213:Cyclins; D004224:Diterpenes; D006027:Glycosides; D017705:Lignans; D010936:Plant Extracts; D016159:Tumor Suppressor Protein p53; C524691:imbricatolic acid; C579580:juniperoside A; D018844:Cyclin-Dependent Kinases