title: Alteration of the proteome profile of the pancreas in diabetic rats induced by streptozotocin.
abstract: Type 1 diabetes mellitus (T1DM) is receiving increased attention. To obtain a better understanding of the mechanism underlying T1DM, we performed a proteomic study on a rat model induced by streptozotocin. Pancreatic proteins were separated by two-dimensional gel electrophoresis. Eighteen protein spots were differentially expressed (P<0.05) with 2-fold or more increased or decreased intensity in the diabetic rats as compared with controls, of which 11 protein spots were up-regulated and 7 protein spots were down-regulated. These protein spots were successfully identified by liquid chromatography-electrospray ionization tandem mass spectrometry. The 60 kDa heat shock protein, the carbonyl reductase 1 (Cbr1), the hydroxyacyl-CoA dehydrogenase, Δ(3,5),Δ(2,4)-dienoyl-CoA isomerase, the elongation factor 1-δ, the 26S protease regulatory subunit 7 and the transitional endoplasmic reticulum ATPase were up-regulated, while the 78 kDa glucose-regulated protein, peroxiredoxin 4 and plakoglobin were down-regulated. The expression change of Cbr1 which is closely related to diabetic complications was further validated by western blotting. Our results and those of the bioinformatics analysis suggest that oxidative stress, the Wnt pathway, fatty acid degradation and glucose transport may be closely related to T1DM.
authors.affiliation: Yue Yang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200030, P.R. China.
authors.forename: Yong-Liang
authors.initials: YL
authors.lastname: Jiang
authors.forename: You
authors.initials: Y
authors.lastname: Ning
authors.forename: Xiao-Lei
authors.initials: XL
authors.lastname: Ma
authors.forename: Yan-Yan
authors.initials: YY
authors.lastname: Liu
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.forename: Zeng
authors.initials: Z
authors.lastname: Zhang
authors.forename: Chun-Xiao
authors.initials: CX
authors.lastname: Shan
authors.forename: Yu-Dong
authors.initials: YD
authors.lastname: Xu
authors.forename: Lei-Miao
authors.initials: LM
authors.lastname: Yin
authors.forename: Yong-Qing
authors.initials: YQ
authors.lastname: Yang
mesh_terms: D000429:Alcohol Oxidoreductases / Q000378:metabolism; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D019295:Computational Biology; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism* / Q000503:physiopathology; D003922:Diabetes Mellitus, Type 1 / Q000378:metabolism / Q000503:physiopathology; D004195:Disease Models, Animal; D015180:Electrophoresis, Gel, Two-Dimensional; D008297:Male; D010179:Pancreas / Q000378:metabolism* / Q000503:physiopathology; D025941:Protein Interaction Mapping; D020543:Proteome; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D001786:Blood Glucose; D020543:Proteome; D000429:Alcohol Oxidoreductases; C531129:CBR1 protein, human