title: Discovery of a small peptide from combinatorial libraries that can activate the plant immune system by a jasmonic acid signaling pathway.
abstract: Plants defend themselves by using an innate immune system that is activated in response to a variety of molecules derived from pathogens. These molecules have provided profound insights into the mechanisms of pathogen recognition and subsequent signaling pathways in plants. In the present study, we screened a combinatorial random hexapeptide library for peptides that activate the plant immune system, by using a cell-based high-throughput screening system in which H(2)O(2) generation was monitored. We discovered a novel small peptide (YGIHTH-amide, PIP-1) that triggered H(2)O(2) production in tobacco and tomato cells, but not in Arabidopsis cells. PIP-1 induced significant levels of phytoalexin biosynthesis and defense-related gene expression in tobacco cells; this is likely to be activated by a jasmonic acid pathway.
authors.affiliation: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan. miyamasa@kais.kyoto-u.ac.jp
authors.forename: Masahiro
authors.initials: M
authors.lastname: Miyashita
authors.forename: Masashi
authors.initials: M
authors.lastname: Oda
authors.forename: Yuji
authors.initials: Y
authors.lastname: Ono
authors.forename: Eriko
authors.initials: E
authors.lastname: Komoda
authors.forename: Hisashi
authors.initials: H
authors.lastname: Miyagawa
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000737:chemistry / Q000378:metabolism*; D020650:Combinatorial Chemistry Techniques; D003517:Cyclopentanes / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000737:chemistry / Q000378:metabolism; D007113:Immunity, Innate / Q000187:drug effects; D018551:Solanum lycopersicum / Q000737:chemistry; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D054883:Oxylipins / Q000378:metabolism*; D057865:Plant Immunity / Q000187:drug effects; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects; D054852:Small Molecule Libraries / Q000737:chemistry; D013329:Structure-Activity Relationship; D014026:Nicotiana / Q000737:chemistry
chemical_list: D003517:Cyclopentanes; D009842:Oligopeptides; D054883:Oxylipins; D054852:Small Molecule Libraries; C011006:jasmonic acid; D006861:Hydrogen Peroxide