title: Isolation of 10 polymorphic microsatellite loci in the marine midge Clunio marinus (Chironomidae, Diptera) and their efficient characterization by heteroduplex analysis.
abstract: Ten polymorphic microsatellite loci were cloned and characterized for the marine midge Clunio marinus (Chironomidae, Diptera). The number of alleles ranged from three to 31, the observed heterozygosity ranged from 0.06 to 0.83. During preliminary tests for polymorphisms, we identified subtly differing alleles due to the heteroduplexes they formed on nondenaturing gels. This allowed for the use of unlabelled primers in standard lower resolution PAGE and thus saved time and money in primer testing.
authors.affiliation: Department of Entomology, Max Planck Institute for Chemical Ecology, Hans-Knoell-Strasse 8, 07745 Jena, Germany.
authors.forename: Tobias S
authors.initials: TS
authors.lastname: Kaiser
authors.forename: David G
authors.initials: DG
authors.lastname: Heckel