title: [Heterogeneity of cell population of lymphoma NK/Ly and leukemia L-1210 according to carbohydrate structure of cell surface: immunocytochemical analysis of lectin binding].
abstract: The heterogeneity of tumor cell populations according to binding of lectins from lentil (LcL), wheat germs (WGA), peanut (PNA) and concanavalin A was investigated on a model of murine Nemeth-Kellner lymphoma (NK/Ly) and leukemia L-1210. Bound lectins were detected by indirect immunochemical method using home obtained polyclonal antilectin antibodies and immunogold silver staining (IGSS) technique. Significant differences in binding of Con A were revealed between NK/Ly (67% Con A+) and L-1210 (7.2% Con A+) cells, while the differences in binding of other lectins with both types of tumor cells were not significant. A relatively high percentage of PNA+ cells was registered that can indicate a high degree of desialization of membrane glycoproteins.
authors.forename: M M
authors.initials: MM
authors.lastname: Lutsik
authors.forename: A M
authors.initials: AM
authors.lastname: Iashchenko
authors.forename: V I
authors.initials: VI
authors.lastname: Kovalishin
authors.forename: O E
authors.initials: OE
authors.lastname: Pridatko
authors.forename: R S
authors.initials: RS
authors.lastname: Stoĭka
authors.forename: M D
authors.initials: MD
authors.lastname: Lutsik
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001202:Ascitic Fluid / Q000473:pathology; D001665:Binding Sites; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000032:analysis*; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism* / Q000648:ultrastructure; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods*; D007202:Indicators and Reagents; D007939:Leukemia L1210 / Q000378:metabolism / Q000473:pathology; D008223:Lymphoma / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008854:Microscopy, Electron; D037121:Plant Lectins / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D002241:Carbohydrates; D007202:Indicators and Reagents; D037121:Plant Lectins