title: 13q14 deletion size and number of deleted cells both influence prognosis in chronic lymphocytic leukemia.
abstract: Deletion at 13q14 is detected by fluorescence in situ hybridization (FISH) in about 50% of chronic lymphocytic leukemia (CLL). Although CLL with 13q deletion as the sole cytogenetic abnormality (del13q-only) usually have good prognosis, more aggressive clinical courses are documented for del13q-only CLL carrying higher percentages of 13q deleted nuclei. Moreover, deletion at 13q of different sizes have been described, whose prognostic significance is still unknown. In a multi-institutional cohort of 342 del13q-only cases and in a consecutive unselected cohort of 265 CLL, we investigated the prognostic significance of 13q deletion, using the 13q FISH probes locus-specific identifier (LSI)-D13S319 and LSI-RB1 that detect the DLEU2/MIR15A/MIR16-1 and RB1 loci, respectively. Results indicated that both percentage of deleted nuclei and presence of larger deletions involving the RB1 locus cooperated to refine the prognosis of del13q-only cases. In particular, CLL carrying <70% of 13q deleted nuclei with deletions not comprising the RB1 locus were characterized by particularly long time-to-treatment. Conversely, CLL with 13q deletion in <70% of nuclei but involving the RB1 locus, or CLL carrying 13q deletion in ≥70% of nuclei, with or without RB1 deletions, collectively experienced shorter time-to-treatment. A revised flowchart for the prognostic FISH assessment of del13q-only CLL, implying the usage of both 13q probes, is proposed.
authors.affiliation: Clinical and Experimental Onco-Hematology Unit, Centro di Riferimento Oncologico, IRCCS, Aviano (PN), Italy.
authors.forename: Michele
authors.initials: M
authors.lastname: Dal Bo
authors.forename: Francesca Maria
authors.initials: FM
authors.lastname: Rossi
authors.forename: Davide
authors.initials: D
authors.lastname: Rossi
authors.forename: Clara
authors.initials: C
authors.lastname: Deambrogi
authors.forename: Francesco
authors.initials: F
authors.lastname: Bertoni
authors.forename: Ilaria
authors.initials: I
authors.lastname: Del Giudice
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Palumbo
authors.forename: Mauro
authors.initials: M
authors.lastname: Nanni
authors.forename: Andrea
authors.initials: A
authors.lastname: Rinaldi
authors.forename: Ivo
authors.initials: I
authors.lastname: Kwee
authors.forename: Erika
authors.initials: E
authors.lastname: Tissino
authors.forename: Giorgia
authors.initials: G
authors.lastname: Corradini
authors.forename: Alessandro
authors.initials: A
authors.lastname: Gozzetti
authors.forename: Emanuele
authors.initials: E
authors.lastname: Cencini
authors.forename: Marco
authors.initials: M
authors.lastname: Ladetto
authors.forename: Angela Maria
authors.initials: AM
authors.lastname: Coletta
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Luciano
authors.forename: Pietro
authors.initials: P
authors.lastname: Bulian
authors.forename: Gabriele
authors.initials: G
authors.lastname: Pozzato
authors.forename: Luca
authors.initials: L
authors.lastname: Laurenti
authors.forename: Francesco
authors.initials: F
authors.lastname: Forconi
authors.forename: Francesco
authors.initials: F
authors.lastname: Di Raimondo
authors.forename: Roberto
authors.initials: R
authors.lastname: Marasca
authors.forename: Giovanni
authors.initials: G
authors.lastname: Del Poeta
authors.forename: Gianluca
authors.initials: G
authors.lastname: Gaidano
authors.forename: Robin
authors.initials: R
authors.lastname: Foà
authors.forename: Anna
authors.initials: A
authors.lastname: Guarini
authors.forename: Valter
authors.initials: V
authors.lastname: Gattei
mesh_terms: D002872:Chromosome Deletion; D025063:Chromosome Disorders / Q000235:genetics*; D002882:Chromosomes, Human, Pair 13 / Q000235:genetics; D015331:Cohort Studies; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics* / Q000473:pathology; D011379:Prognosis; D062085:RNA, Long Noncoding; D016160:Retinoblastoma Protein / Q000235:genetics; D017384:Sequence Deletion; D014166:Transferases; D025521:Tumor Suppressor Proteins / Q000235:genetics
chemical_list: C109420:DLEU2 lncRNA, human; D062085:RNA, Long Noncoding; D016160:Retinoblastoma Protein; D025521:Tumor Suppressor Proteins; D014166:Transferases