title: PERMANENT GENETIC RESOURCES: Eighteen new polymorphic microsatellite markers for the endangered Florida manatee, Trichechus manatus latirostris.
abstract: Here we describe 18 polymorphic microsatellite loci for Trichechus manatus latirostris (Florida manatee), isolated using a polymerase chain reaction-based technique. The number of alleles at each locus ranged from two to four (mean = 2.5) in specimens from southwest (n = 58) and northeast (n = 58) Florida. Expected and observed heterozygosities ranged from 0.11 to 0.67 (mean = 0.35) and from 0.02 to 0.78 (mean = 0.34), respectively. Departures from Hardy-Weinberg equilibrium occurred at two loci. There was no evidence of genotypic disequilibrium for any pair of loci. For individual identification, mean random-mating and θ-corrected match probabilities were 9.36 × 10(-7) and 1.95 × 10(-6) , respectively.
authors.affiliation: Florida Fish and Wildlife Research Institute, 100 Eighth Avenue S.E. Saint Petersburg, FL 33701-5095, USA, Mote Marine Laboratory, 1600 Thompson Parkway, Sarasota, FL 34236-1004, USA.
authors.forename: Michael D
authors.initials: MD
authors.lastname: Tringali
authors.forename: Seifu
authors.initials: S
authors.lastname: Seyoum
authors.forename: Susan L
authors.initials: SL
authors.lastname: Carney
authors.forename: Michelle C
authors.initials: MC
authors.lastname: Davis
authors.forename: Marta A
authors.initials: MA
authors.lastname: Rodriguez-Lopez
authors.forename: John E
authors.initials: JE
authors.lastname: Reynolds Iii
authors.forename: Elsa
authors.initials: E
authors.lastname: Haubold