title: Isolation of sixteen autosomal loci and a sex-linked polymorphic microsatellite locus from the Milne-Edwards' sportive lemur (Lepilemur edwardsi).
abstract: We isolated 21 microsatellites from the Milne-Edwards' sportive lemur, Lepilemur edwardsi. Eighteen microsatellite sequences possessed sufficient flanking DNA for primer design. Seventeen loci amplified and were found to be polymorphic displaying two to 17 alleles in 32 unrelated individuals from a population from the National Park of Ankarafantsika in northwest Madagascar. One locus (Led-12) was found to be sex linked located on the X chromosome and can be used to sex-type 40% of female L. edwardsi lemurs. These 17 loci were characterized to investigate family structure and the phylogeography of L. edwardsi.
authors.affiliation: NERC Molecular Genetics Facility, Department of Animal and Plant Sciences, University of Sheffield, Sheffield S10 2TN, UK; Institute of Zoology, University of Veterinary Medicine Hannover, Buenteweg 17, 30559 Hannover, Germany; Cardiff School of Biosciences, Cardiff University, Cardiff, CF10 3TL, Wales, UK.
authors.forename: M
authors.initials: M
authors.lastname: Craul
authors.forename: D A
authors.initials: DA
authors.lastname: Dawson
authors.forename: U
authors.initials: U
authors.lastname: Radespiel
authors.forename: E
authors.initials: E
authors.lastname: Zimmermann
authors.forename: M W
authors.initials: MW
authors.lastname: Bruford