title: Application of DNA markers linked to the potato H1 gene conferring resistance to pathotype Ro1 of Globodera rostochiensis.
abstract: Ninety-one potato genotypes (cultivars and breeding lines) selected as resistant or susceptible to pathotype Ro1 of Globodera rostochiensis were screened for the presence of two PCR markers, 0.14 and 0.76 kb in length. Both PCR markers were linked with the H1 gene, located at the distal end of the long arm of chromosome V, and were present in 88 to 100% of the resistant cultivars and breeding lines. The 0.76 kb PCR marker was detected in all resistant genotypes and in approximately 86% of susceptible breeding lines as well as in all susceptible cultivars. The 0.14 kb marker was detected in 88% of resistant breeding lines and in 94% of resistant cultivars. Most of the susceptible genotypes tested (91% of cultivars, but only 50% of breeding lines) did not show the presence of the 0.14 kb marker. We conclude that the 0.14 kb H1 marker is likely to be useful for the proper selection of potato genotypes resistant to the Ro1 pathotype of G. rostochiensis.
authors.affiliation: Department of Genetics, Plant Breeding and Seed Production, Wroclaw University of Environmental and Life Sciences, Poland.
authors.forename: Renata
authors.initials: R
authors.lastname: Galek
authors.forename: Michał
authors.initials: M
authors.lastname: Rurek
authors.forename: Walter S
authors.initials: WS
authors.lastname: De Jong
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Pietkiewicz
authors.forename: Halina
authors.initials: H
authors.lastname: Augustyniak
authors.forename: Ewa
authors.initials: E
authors.lastname: Sawicka-Sienkiewicz
mesh_terms: D000818:Animals; D001947:Breeding; D060467:Disease Resistance / Q000235:genetics*; D017343:Genes, Plant; D005819:Genetic Markers; D005838:Genotype; D011198:Solanum tuberosum / Q000235:genetics* / Q000469:parasitology; D014431:Tylenchoidea
chemical_list: D005819:Genetic Markers