title: The neural circuits and sensory channels mediating harsh touch sensation in Caenorhabditis elegans.
abstract: Most animals can distinguish two distinct types of touch stimuli: gentle (innocuous) and harsh (noxious/painful) touch, however, the underlying mechanisms are not well understood. Caenorhabditis elegans is a useful model for the study of gentle touch sensation. However, little is known about harsh touch sensation in this organism. Here we characterize harsh touch sensation in C. elegans. We show that C. elegans exhibits differential behavioural responses to harsh touch and gentle touch. Laser ablations identify distinct sets of sensory neurons and interneurons required for harsh touch sensation at different body segments. Optogenetic stimulation of the circuitry can drive behaviour. Patch-clamp recordings reveal that TRP family and amiloride-sensitive Na(+) channels mediate touch-evoked currents in different sensory neurons. Our work identifies the neural circuits and characterizes the sensory channels mediating harsh touch sensation in C. elegans, establishing it as a genetic model for studying this sensory modality.
authors.affiliation: Life Sciences Institute, University of Michigan, Ann Arbor, Michigan 48109, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Lijun
authors.initials: L
authors.lastname: Kang
authors.forename: Beverly J
authors.initials: BJ
authors.lastname: Piggott
authors.forename: Zhaoyang
authors.initials: Z
authors.lastname: Feng
authors.forename: X Z Shawn
authors.initials: XZ
authors.lastname: Xu
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics / Q000502:physiology*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism*; D007395:Interneurons / Q000502:physiology*; D011984:Sensory Receptor Cells / Q000502:physiology*; D015222:Sodium Channels / Q000235:genetics / Q000378:metabolism*; D014110:Touch; D050051:Transient Receptor Potential Channels / Q000235:genetics / Q000378:metabolism*
chemical_list: D029742:Caenorhabditis elegans Proteins; D015222:Sodium Channels; D050051:Transient Receptor Potential Channels