title: Antibodies against Helicobacter pylori heat shock protein 60 aggravate HSP60-mediated proinflammatory responses.
abstract: Anti-Helicobacter pylori heat shock protein 60 (HpHSP60) antibodies are usually found in H. pylori-infected patients and are known to be associated with the progression of gastric diseases. However, the effects of these antibodies on the functions of HpHSP60 have not been identified. This study aims to investigate the effects of the interaction between anti-HSP60 antibodies and HpHSP60 on inflammatory responses. Anti-HpHSP60 polyclonal sera and monoclonal antibodies (mAbs) were produced to evaluate their effects on HpHSP60-induced IL-8 and TNF-α activity. The results indicated that anti-HpHSP60 polyclonal sera collected from patients infected with H. pylori or from rabbit and mice immunized with HpHSP60 could significantly enhance HpHSP60-mediated IL-8 and TNF-α secretion from monocytic THP-1 cells. Similar effects were also found with anti-HpHSP60 mAbs. Further analysis revealed that this phenomenon was only carried out by anti-HpHSP60 antibody but not by other non-specific mAbs. Moreover, the non-specific mAbs decreased the synergism of HpHSP60 and anti-HpHSP60 mAbs in proinflammatory cytokine induction. Herein, we have examined the role of anti-HpHSP60 antibody in host immune responses for the first time. This study demonstrated that H. pylori HSP60/mAbs could modulate helicobacterial pathogenesis by increasing IL-8 and TNF-α production. The pathogen-specific antibodies may execute potential immune functions rather than recognize or neutralize microbes.
authors.affiliation: Department of Biological Science and Technology and Institute of Molecular Medicine and Bioengineering, National Chiao Tung University, Hsinchu, Taiwan.
authors.forename: Kuang-Wen
authors.initials: KW
authors.lastname: Liao
authors.forename: Chen-Si
authors.initials: CS
authors.lastname: Lin
authors.forename: Wen-Liang
authors.initials: WL
authors.lastname: Chen
authors.forename: Chu-Ting
authors.initials: CT
authors.lastname: Yang
authors.forename: Ching-Min
authors.initials: CM
authors.lastname: Lin
authors.forename: Wei-Tung
authors.initials: WT
authors.lastname: Hsu
authors.forename: Yi-Yin
authors.initials: YY
authors.lastname: Lin
authors.forename: Yi-Han
authors.initials: YH
authors.lastname: Chiu
authors.forename: Kuo-Chin
authors.initials: KC
authors.lastname: Huang
authors.forename: Hsin-Yi
authors.initials: HY
authors.lastname: Wu
authors.forename: Ming-Shiang
authors.initials: MS
authors.lastname: Wu
authors.forename: Chang-Jer
authors.initials: CJ
authors.lastname: Wu
authors.forename: Simon Jen-Tan
authors.initials: SJ
authors.lastname: Mao
authors.forename: Nu-Man
authors.initials: NM
authors.lastname: Tsai
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology*; D002460:Cell Line; D018834:Chaperonin 60 / Q000276:immunology*; D005260:Female; D016481:Helicobacter Infections / Q000276:immunology; D016480:Helicobacter pylori / Q000276:immunology* / Q000472:pathogenicity; D006801:Humans; D007249:Inflammation / Q000276:immunology*; D016209:Interleukin-8 / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits; D011961:Receptors, Fc / Q000276:immunology; D015398:Signal Transduction / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
chemical_list: D000906:Antibodies; D001426:Bacterial Proteins; D018834:Chaperonin 60; D016209:Interleukin-8; D011961:Receptors, Fc; D014409:Tumor Necrosis Factor-alpha