title: Prevention of Phormidium tenue Biofilm Formation by TiO(2) Photocatalysis.
abstract: We showed that the photocatalytic effect of a coating of TiO(2) greatly reduces the formation of a biofilm by Phormidium tenue (P. tenue), a filamentous cyanobacterium, on glass plates. Sample plates were immersed in P. tenue culture solution (OD(730)=0.3) under concurrent illumination with white fluorescent (WF) and UV light (0.3 mW cm(-2), each) for 11 days. TiO(2)-coated glass plates showed greatly reduced adhesion of P. tenue over 11 days compared to bare plates. The number of P. tenue adhering to bare glass plates increased to over 10(6) cells cm(-2) in 6 days. The photocatalytic anti-biofilm effect was also observed under WF light, although it was small and lasted only a few days. The addition of 1 mM mannitol, a scavenger for the hydroxyl radical (·OH), suppressed the effect. The surface of TiO(2)-coated plates was maintained in a highly hydrophilic state for 11 days, regardless of the addition of mannitol. Therefore, we conclude that the photocatalytic oxidation of P. tenue is effective in preventing the formation of a biofilm.
authors.affiliation: Research Institute for Environmental Management Technology, National Institute of Advanced Industrial Science and Technology (AIST).
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Ohko
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Nagao
authors.forename: Kunihiro
authors.initials: K
authors.lastname: Okano
authors.forename: Norio
authors.initials: N
authors.lastname: Sugiura
authors.forename: Akari
authors.initials: A
authors.lastname: Fukuda
authors.forename: Yingnan
authors.initials: Y
authors.lastname: Yang
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Negishi
authors.forename: Mio
authors.initials: M
authors.lastname: Takeuchi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Hanada