title: Pentapeptide-modified poly(N,N-diethylacrylamide) hydrogel scaffolds for tissue engineering.
abstract: Poly(N,N-diethylacrylamide) (PDEAAm) hydrogel scaffolds were prepared by radical copolymerization of N,N-diethylacrylamide (DEAAm), N,N'-methylenebisacrylamide and methacrylic acid in the presence of (NH₄)₂SO₄ or NaCl. The hydrogels were characterized by low-vacuum scanning electron microscopy in the water-swollen state, water and cyclohexane regain, and by mercury porosimetry. The pentapeptide, YIGSR-NH₂, was immobilized on the hydrogel. Human embryonic stem cells (hESCs) were cultured with the hydrogels to test their biocompatibility. The results suggest that the PDEAAm hydrogel scaffolds are nontoxic and support hESC attachment and proliferation, and that interconnected pores of the scaffolds are important for hESC cultivation. Immobilization of YIGSR-NH₂ pentapeptide on the PDEAAm surface improved both adhesion and growth of hESCs compared with the unmodified hydrogel. The YIGSR-NH₂-modified PDEAAm hydrogels may be a useful tool for tissue-engineering purposes.
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, 16206 Prague 6, Czech Republic. horak@imc.cas.cz
authors.forename: Daniel
authors.initials: D
authors.lastname: Horák
authors.forename: Kamil
authors.initials: K
authors.lastname: Matulka
authors.forename: Helena
authors.initials: H
authors.lastname: Hlídková
authors.forename: Monika
authors.initials: M
authors.lastname: Lapčíková
authors.forename: Milan J
authors.initials: MJ
authors.lastname: Beneš
authors.forename: Josef
authors.initials: J
authors.lastname: Jaroš
authors.forename: Aleš
authors.initials: A
authors.lastname: Hampl
authors.forename: Petr
authors.initials: P
authors.lastname: Dvořák
mesh_terms: D000178:Acrylamides / Q000737:chemistry*; D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation; D053595:Embryonic Stem Cells / Q000166:cytology*; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D051379:Mice; D009842:Oligopeptides / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D000178:Acrylamides; D020100:Hydrogels; D009842:Oligopeptides; D011108:Polymers; C428028:poly(N,N-diethylacrylamide)