title: Quantification of asenapine and three metabolites in human plasma using liquid chromatography-tandem mass spectrometry with automated solid-phase extraction: application to a phase I clinical trial with asenapine in healthy male subjects.
abstract: The development and validation of methods for determining concentrations of the antipsychotic drug asenapine (ASE) and three of its metabolites [N-desmethylasenapine (DMA), asenapine-N(+) -glucuronide (ASG) and 11-O-sulfate-asenapine (OSA)] in human plasma using LC-MS/MS with automated solid-phase extraction is described. The three assessment methods in human plasma were found to be acceptable for quantification in the ranges 0.0250-20.0 ng/mL (ASE), 0.0500-20.0 ng/mL (DMA and OSA) and 0.250-50.0 ng/mL (ASG).
authors.affiliation: QPS Netherlands, Petrus Campersingel 123, 9713 AG Groningen, The Netherlands. theo.de.boer@qps.com
authors.forename: Theo
authors.initials: T
authors.lastname: de Boer
authors.forename: Erik
authors.initials: E
authors.lastname: Meulman
authors.forename: Henri
authors.initials: H
authors.lastname: Meijering
authors.forename: Jaap
authors.initials: J
authors.lastname: Wieling
authors.forename: Peter
authors.initials: P
authors.lastname: Dogterom
authors.forename: Holger
authors.initials: H
authors.lastname: Lass
mesh_terms: D000328:Adult; D002853:Chromatography, Liquid / Q000379:methods*; D003986:Dibenzocycloheptenes; D004311:Double-Blind Method; D004355:Drug Stability; D006576:Heterocyclic Compounds, 4 or More Rings / Q000097:blood* / Q000378:metabolism / Q000493:pharmacokinetics; D006801:Humans; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D003986:Dibenzocycloheptenes; D006576:Heterocyclic Compounds, 4 or More Rings; C522667:asenapine