title: Modelling the growth and ethanol production of Brettanomyces bruxellensis at different glucose concentrations.
abstract: OBJECTIVE
  To study the effect of glucose concentrations on the growth by Brettanomyces bruxellensis yeast strain in batch experiments and develop a mathematical model for kinetic behaviour analysis of yeast growing in batch culture.
  
  
  RESULTS
  A Matlab algorithm was developed for the estimation of model parameters. Glucose fermentation by B. bruxellensis was studied by varying its concentration (5, 9.3, 13.8, 16.5, 17.6 and 21.4%). The increase in substrate concentration up to a certain limit was accompanied by an increase in ethanol and biomass production; at a substrate concentration of 50-138 g l(-1), the ethanol and biomass production were 24, 59 and 6.3, 11.4 g l(-1), respectively. However, an increase in glucose concentration to 165 g l(-1) led to a drastic decrease in product formation and substrate utilization.
  
  
  CONCLUSIONS
  The model successfully simulated the batch kinetic observed in all cases. The confidence intervals were also estimated at each phase at a 0.95 probability level in a t-Student distribution for f degrees of freedom. The maximum ethanol and biomass yields were obtained with an initial glucose concentration of 138 g l(-1).
  
  
  CONCLUSIONS
  These experiments illustrate the importance of using a mathematical model applied to kinetic behaviour on glucose concentration by B. bruxellensis.
authors.affiliation: Departamento Ing. Elétrica y Electrónica, Instituto Tecnológico de Veracruz, Unidad de Invesitgación y Desarrollo en Alimentos, Veracruz, Ver, México. maguilaruscanga@yahoo.com.mx
authors.forename: M G
authors.initials: MG
authors.lastname: Aguilar-Uscanga
authors.forename: Y
authors.initials: Y
authors.lastname: Garcia-Alvarado
authors.forename: J
authors.initials: J
authors.lastname: Gomez-Rodriguez
authors.forename: T
authors.initials: T
authors.lastname: Phister
authors.forename: M L
authors.initials: ML
authors.lastname: Delia
authors.forename: P
authors.initials: P
authors.lastname: Strehaiano
mesh_terms: D061249:Batch Cell Culture Techniques; D018533:Biomass; D055168:Brettanomyces / Q000378:metabolism*; D004271:DNA, Fungal; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005516:Food Microbiology; D005947:Glucose / Q000378:metabolism*; D008954:Models, Biological
chemical_list: D004271:DNA, Fungal; D000431:Ethanol; D005947:Glucose