title: Mutations in an alternatively spliced exon of h-ras are not associated with loss of heterozygosity on chromosome 11p15.5 in ovarian tumorigenesis.
abstract: There is evidence that an alternatively spliced exon of the H-ras gene, called idx is associated with down-regulation of H-ras activity. We tested the hypothesis that mutations in this exon play an important role in the development of ovarian carcinomas because loss of heterozygosity at the H-ras locus is frequently observed in these tumors. The idx sequence of 26 different ovarian carcinomas was amplified by PCR and the products were analyzed for possible mutations by single-stranded conformation polymorphism and DNA sequencing. The results showed no idx mutations, even in tumors with a demonstrable loss of one H-ras allele.
authors.affiliation: UNIV SO CALIF,KENNETH NORRIS JR COMPREHENS CANC CTR,DEPT PATHOL,LOS ANGELES,CA 90033. UNIV SO CALIF,KENNETH NORRIS JR COMPREHENS CANC CTR,DEPT GYNECOL ONCOL,LOS ANGELES,CA 90033.
authors.forename: R
authors.initials: R
authors.lastname: Friedman
authors.forename: M
authors.initials: M
authors.lastname: Velicescu
authors.forename: L
authors.initials: L
authors.lastname: Dubeau