title: Mineral trioxide aggregate promotes odontoblastic differentiation via mitogen-activated protein kinase pathway in human dental pulp stem cells.
abstract: Mitogen-activated protein kinase (MAPK) pathways are involved in stem cell differentiation. However, the odontoblastic differentiation-inducing effects by mineral trioxide aggregate (MTA) via MAPK pathways have not been clarified in human dental pulp stem cells (DPSCs). In this study we investigated the effects of MTA on cell viability and production of differentiation markers, and the involvement of MAPK signaling pathways in cultured human DPSCs. Cells were cultured with MTA, and the viability and differentiation productions of the cells were determined using the MTT assay and real-time PCR analysis, respectively. MAPK activation was measured by western blotting. MTA at concentrations of 20 and 10 mg/ml was toxic for human DPSCs. MTA significantly increased the expression of alkaline phosphatase (ALP), dentin sialophosphoprotein (DSPP), type I collagen (COLI), osteocalcin (OCN) and bone sialoprotein (BSP) mRNAs and induced the phosphorylation of p42 and p44 (p42/44), p38 and c-Jun N-terminal kinases 1 and 2 (JNK1/2) MAPK. Furthermore, the inhibitor of p42/44 MAPK attenuated the MTA-induced odontoblastic differentiation. These data indicated that MTA-induced odontoblastic differentiation of human DPSCs was via MAPK pathways, which may play a key role in the repair responses of dentin-pulp-like complexes.
authors.affiliation: Department of Operative Dentistry & Endodontics, School of Stomatology, Fourth Military Medical University, and Department of Hepatobiliary Surgery, Xijing Hospital, Xi'an, 710032, People's Republic of China.
authors.forename: Xiu
authors.initials: X
authors.lastname: Zhao
authors.forename: Wenxi
authors.initials: W
authors.lastname: He
authors.forename: Zhi
authors.initials: Z
authors.lastname: Song
authors.forename: Zhongchun
authors.initials: Z
authors.lastname: Tong
authors.forename: Shiting
authors.initials: S
authors.lastname: Li
authors.forename: Longxing
authors.initials: L
authors.lastname: Ni
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D017607:Aluminum Compounds / Q000494:pharmacology*; D015153:Blotting, Western; D017610:Calcium Compounds / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects* / Q000502:physiology; D024042:Collagen Type I / Q000378:metabolism; D003782:Dental Pulp / Q000166:cytology*; D004338:Drug Combinations; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D006801:Humans; D058574:Integrin-Binding Sialoprotein / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D009804:Odontoblasts / Q000502:physiology*; D015675:Osteocalcin / Q000378:metabolism; D010087:Oxides / Q000494:pharmacology*; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation; D060888:Real-Time Polymerase Chain Reaction; D012795:Sialoglycoproteins / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects* / Q000502:physiology; D017640:Silicates / Q000494:pharmacology*; D013234:Stem Cells / Q000502:physiology*; D013778:Tetrazolium Salts; D013844:Thiazoles; D000099311:Dentin Sialophosphoprotein
chemical_list: D000469:Alkaline Phosphatase; D017607:Aluminum Compounds; D017610:Calcium Compounds; D024042:Collagen Type I; D004338:Drug Combinations; D016326:Extracellular Matrix Proteins; D058574:Integrin-Binding Sialoprotein; D020928:Mitogen-Activated Protein Kinases; D015675:Osteocalcin; D010087:Oxides; D010750:Phosphoproteins; D012795:Sialoglycoproteins; D017640:Silicates; D013778:Tetrazolium Salts; D013844:Thiazoles; D000099311:Dentin Sialophosphoprotein; C086631:mineral trioxide aggregate; C022616:thiazolyl blue