title: Approaches for cytogenetic and molecular analyses of small flow-sorted cell populations from childhood leukemia bone marrow samples.
abstract: Discordances between minimal residual disease estimates obtained by different methods are a problem in childhood acute lymphoblastic leukemia. We aimed to optimize methods allowing the biological exploration of such discrepancies, i.e. the combination of flow-sorting of small immunophenotypically defined cell populations with subsequent analyses of leukemia-associated cytogenetic and molecular marker. The approaches described here optimize the use of the same tube of unfixed, antibody-stained BM cells for flow-sorting of small cell populations and subsequent exploratory FISH and PCR-based analyses.
authors.affiliation: Department of Clinical Immunology, section 7631, University Hospital Rigshospitalet, Copenhagen, Denmark.
authors.forename: Nina Friesgaard
authors.initials: NF
authors.lastname: Obro
authors.forename: Hans Ole
authors.initials: HO
authors.lastname: Madsen
authors.forename: Lars Peter
authors.initials: LP
authors.lastname: Ryder
authors.forename: Mette Klarskov
authors.initials: MK
authors.lastname: Andersen
authors.forename: Kjeld
authors.initials: K
authors.lastname: Schmiegelow
authors.forename: Hanne Vibeke
authors.initials: HV
authors.lastname: Marquart
mesh_terms: D001853:Bone Marrow / Q000737:chemistry* / Q000276:immunology; D002469:Cell Separation / Q000379:methods*; D002648:Child; D003582:Cytogenetics / Q000379:methods*; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D018365:Neoplasm, Residual / Q000737:chemistry / Q000276:immunology; D016133:Polymerase Chain Reaction / Q000379:methods*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D011948:Receptors, Antigen, T-Cell / Q000276:immunology
chemical_list: D007074:Immunoglobulin G; D011948:Receptors, Antigen, T-Cell