title: No association between polymorphisms/haplotypes of the vascular endothelial growth factor gene and preeclampsia.
abstract: BACKGROUND
  Preeclampsia (PE) is the first worldwide cause of death in pregnant women, intra-uterine growth retardation, and fetal prematurity. Some vascular endothelial grown factor gene (VEGF) polymorphisms have been associated to PE and other pregnancy disturbances. We evaluated the associations between VEGF genotypes/haplotypes and PE in Mexican women.
  
  
  METHODS
  164 pregnant women were enrolled in a case-control study (78 cases and 86 normotensive pregnant controls). The rs699947 (-2578C/A), rs1570360 (-1154G/A), rs2010963 (+405G/C), and rs25648 (-7C/T), VEGF variants were discriminated using Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR-RFLP) methods or Taqman single nucleotide polymorphism (SNP) assays.
  
  
  RESULTS
  The proportions of the minor allele for rs699947, rs1570360, rs2010963, and rs25648 VEGF SNPs were 0.33, 0.2, 0.39, and 0.17 in controls, and 0.39, 0.23, 0.41, and 0.15 in cases, respectively (P values > 0.05). The most frequent haplotypes of rs699947, rs1570360, rs2010963, and rs25648 VEGF SNPs, were C-G-C-C and C-G-G-C with frequencies of 0.39, 0.21 in cases and 0.37, 0.25 in controls, respectively (P values > 0.05)
  
  
  CONCLUSIONS
  There was no evidence of an association between VEGF alleles, genotypes, or haplotypes frequencies and PE in our study.
authors.affiliation: Laboratorio de Medicina Molecular, Unidad Academica de Medicina Humana y Ciencias de la Salud, Universidad Autonoma de Zacatecas, Carretera Zacatecas-Guadalajara Km,6, Ejido la Escondida, Zacatecas, Mexico.
authors.forename: Idalia
authors.initials: I
authors.lastname: Garza-Veloz
authors.forename: Claudia
authors.initials: C
authors.lastname: Castruita-De la Rosa
authors.forename: Raul
authors.initials: R
authors.lastname: Cortes-Flores
authors.forename: Victoria
authors.initials: V
authors.lastname: Martinez-Gaytan
authors.forename: Jose E
authors.initials: JE
authors.lastname: Rivera-Muñoz
authors.forename: Elda A
authors.initials: EA
authors.lastname: Garcia-Mayorga
authors.forename: Esteban
authors.initials: E
authors.lastname: Meza-Lamas
authors.forename: Augusto
authors.initials: A
authors.lastname: Rojas-Martinez
authors.forename: Rocio
authors.initials: R
authors.lastname: Ortiz-Lopez
authors.forename: Margarita L
authors.initials: ML
authors.lastname: Martinez-Fierro
mesh_terms: D000293:Adolescent; D000328:Adult; D000483:Alleles; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D006239:Haplotypes; D006801:Humans; D008800:Mexico; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011225:Pre-Eclampsia / Q000235:genetics*; D011247:Pregnancy; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*; D055815:Young Adult
chemical_list: D042461:Vascular Endothelial Growth Factor A