title: DArT markers tightly linked with the Rfc1 gene controlling restoration of male fertility in the CMS-C system in cultivated rye (Secale cereale L.).
abstract: The Rfc1 gene controls restoration of male fertility in rye (Secale cereale L.) with sterility-inducing cytoplasm CMS-C. Two populations of recombinant inbred lines (RIL) were used in this study to identify DArT markers located on the 4RL chromosome, in the close vicinity of the Rfc1 gene. In the population developed from the 541×2020LM intercross, numerous markers tightly linked with the restorer gene were identified. This group contained 91 DArT markers and three SCARs additionally analyzed in the study. All these markers were mapped in the distance not exceeding 6 cM from the gene of interest. In the second mapping population (541×Ot1-3 intercross), only 9 DArT markers located closely to the Rfc1 gene were identified. Five of these DArT markers were polymorphic in both populations.
authors.affiliation: Department of Plant Genetics, Breeding and Biotechnology, West-Pomeranian University of Technology, Szczecin, Poland. sstojalowski@zut.edu.pl
authors.forename: Stefan Andrzej
authors.initials: SA
authors.lastname: Stojałowski
authors.forename: Paweł
authors.initials: P
authors.lastname: Milczarski
authors.forename: Monika
authors.initials: M
authors.lastname: Hanek
authors.forename: Hanna
authors.initials: H
authors.lastname: Bolibok-Brągoszewska
authors.forename: Beata
authors.initials: B
authors.lastname: Myśków
authors.forename: Andrzej
authors.initials: A
authors.lastname: Kilian
authors.forename: Monika
authors.initials: M
authors.lastname: Rakoczy-Trojanowska
mesh_terms: D002874:Chromosome Mapping; D003433:Crosses, Genetic; D003593:Cytoplasm / Q000235:genetics; D005298:Fertility; D017343:Genes, Plant; D008040:Genetic Linkage; D005819:Genetic Markers; D007246:Infertility; D051479:Plant Infertility / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D012434:Secale / Q000235:genetics*
chemical_list: D005819:Genetic Markers; D010940:Plant Proteins