title: [Effect of TPT1 transduction on the biological behaviors of liver cell lines].
abstract: OBJECTIVE
  The aim of this study was to transfect TPT1 into cell lines SMMC-7721 and L-02, seperately, and to observe the changes of biological behaviors of the cell lines.
  
  
  METHODS
  Through lipofectamine, the eukaryotic report expression vector containing TPT1 ORF (open reading frame), pEGFP-N3TPT1, were transducted into hepatocarcinoma cell line SMMC-7721 cells and normal liver cell line L-02 cells, seperately. The transduction was repeated three times in 24 hrs. The differences of biological behaviors between the pEGFP-N3TPT1 and pEGFP-N3 groups were studied by RT-PCR, MTT assay, soft agar colony formation assay and cell cycle analysis.
  
  
  RESULTS
  The pEGFP-N3TPT1 transfected cells had a high mRNA level in the two cell lines (P < 0.05) compared with the pEGFP-N3 controls. The ability of proliferation and the soft agar colony formation were enhanced in the SMMC-7721 transducted cells with pEGFP-N3TPT1 compared with that transducted with pEGFP-N3 (P < 0.05), and the cell cycle analysis showed that the cells in the phase G(2)+S/M increased after pEGFP-N3TPT1 transduction. In the L-02 cell line, we obtained similar results, pEGFP-N3TPT1 enhanced the colony formation in plate (P < 0.05), but not make it form colony in soft agar.
  
  
  CONCLUSIONS
  TPT1 can enhance malignant phenotype of SMMC-7721 cells and promote the growth of L-02 cells, but not transform L-02 into malignant phenotype.
authors.affiliation: Department of Oncology, Henan Provincial People's Hospital, Zhengzhou 450003, China. gaotianhui@yahoo.com.cn
authors.forename: Tian-hui
authors.initials: TH
authors.lastname: Gao
authors.forename: Fang-ling
authors.initials: FL
authors.lastname: Duan
authors.forename: Ming-yue
authors.initials: MY
authors.lastname: Liu
authors.forename: Xiao-yan
authors.initials: XY
authors.lastname: Li
mesh_terms: D014408:Biomarkers, Tumor / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology*; D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000473:pathology*; D002453:Cell Cycle; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology*; D016366:Open Reading Frames; D012333:RNA, Messenger / Q000378:metabolism; D014162:Transfection; D000090324:Tumor Protein, Translationally-Controlled 1
chemical_list: D014408:Biomarkers, Tumor; D012333:RNA, Messenger; C000715647:TPT1 protein, human; D000090324:Tumor Protein, Translationally-Controlled 1; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins