title: Single nucleotide polymorphisms in collagenous lectins and other innate immune genes in pigs with common infectious diseases.
abstract: Innate immune recognition of pathogens involves various surface receptors and soluble proteins that precede agglutination, complement activation, phagocytosis, and the adaptive immune response. Mannan-binding lectins (MBLs), ficolins (FCNs) and surfactant protein A (SP-A) are soluble collagenous lectins that bind surface structures of various bacteria, viruses and fungi. Some single nucleotide polymorphisms (SNPs) in collagenous lectin genes of humans and other species, including pigs, have been implicated in variation in susceptibility to infectious and inflammatory diseases. In this study we determined the frequencies of 13 SNP alleles of MBL-A, MBL-C, ficolin-α, ficolin-β, and SP-A in 1324 healthy pigs and 461 pigs diagnosed with common infectious diseases at necropsy. For comparison, we also analyzed 12 other SNP alleles in several other innate immune genes, including galectins and TLRs. Several SNPs within genes encoding porcine MBL-A, MBL-C and SP-A were more frequent in pigs diagnosed at necropsy with various diseases or pathogens. These findings suggest that several collagenous lectin SNPs are associated with disease susceptibility and therefore might be genetic markers of impaired innate immune function.
authors.affiliation: Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1, Canada.
authors.forename: N D
authors.initials: ND
authors.lastname: Keirstead
authors.forename: M A
authors.initials: MA
authors.lastname: Hayes
authors.forename: G E
authors.initials: GE
authors.lastname: Vandervoort
authors.forename: A S
authors.initials: AS
authors.lastname: Brooks
authors.forename: E J
authors.initials: EJ
authors.lastname: Squires
authors.forename: B N
authors.initials: BN
authors.lastname: Lillie
mesh_terms: D000818:Animals; D037222:Collectins / Q000235:genetics*; D003141:Communicable Diseases / Q000235:genetics / Q000276:immunology / Q000382:microbiology / Q000662:veterinary* / Q000821:virology; D037541:Galectin 4 / Q000235:genetics; D005838:Genotype; D007113:Immunity, Innate / Q000235:genetics* / Q000276:immunology; D037102:Lectins / Q000235:genetics; D050606:Mannose-Binding Protein-Associated Serine Proteases / Q000235:genetics; D020641:Polymorphism, Single Nucleotide / Q000235:genetics* / Q000276:immunology; D013552:Swine / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D013553:Swine Diseases / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D000097604:Ficolins
chemical_list: D037222:Collectins; D037541:Galectin 4; D037102:Lectins; D050606:Mannose-Binding Protein-Associated Serine Proteases; D000097604:Ficolins