title: The PurR regulon in Escherichia coli K-12 MG1655.
abstract: The PurR transcription factor plays a critical role in transcriptional regulation of purine metabolism in enterobacteria. Here, we elucidate the role of PurR under exogenous adenine stimulation at the genome-scale using high-resolution chromatin immunoprecipitation (ChIP)-chip and gene expression data obtained under in vivo conditions. Analysis of microarray data revealed that adenine stimulation led to changes in transcript level of about 10% of Escherichia coli genes, including the purine biosynthesis pathway. The E. coli strain lacking the purR gene showed that a total of 56 genes are affected by the deletion. From the ChIP-chip analysis, we determined that over 73% of genes directly regulated by PurR were enriched in the biosynthesis, utilization and transport of purine and pyrimidine nucleotides, and 20% of them were functionally unknown. Compared to the functional diversity of the regulon of the other general transcription factors in E. coli, the functions and size of the PurR regulon are limited.
authors.affiliation: Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA.
authors.forename: Byung-Kwan
authors.initials: BK
authors.lastname: Cho
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Federowicz
authors.forename: Mallory
authors.initials: M
authors.lastname: Embree
authors.forename: Young-Seoub
authors.initials: YS
authors.lastname: Park
authors.forename: Donghyuk
authors.initials: D
authors.lastname: Kim
authors.forename: Bernhard Ø
authors.initials: BØ
authors.lastname: Palsson
mesh_terms: D000225:Adenine / Q000494:pharmacology; D001665:Binding Sites; D047369:Chromatin Immunoprecipitation; D048168:Escherichia coli K12 / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D017353:Gene Deletion; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D016680:Genome, Bacterial; D019271:Hypoxanthine / Q000378:metabolism; D053858:Metabolic Networks and Pathways / Q000235:genetics; D018085:Regulon; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
chemical_list: D029968:Escherichia coli Proteins; C489317:PurR protein, E coli; D012097:Repressor Proteins; D019271:Hypoxanthine; D000225:Adenine