title: A novel biosensor based on glucose oxidase for activity determination of α - amylase.
abstract: A glucose oxidase-based biosensor was developed for the determination of α-amylase activity. The determination method is based on monitoring the decrease in dissolved oxygen concentration related to the starch concentration, for which starch gives a reaction with α-amylase. Optimization parameters, including glucose oxidase amount, gelatin amount, and glutaraldehyde percentage for cross-linking, were investigated. The effects of pH, buffer system, and temperature on the biosensor system were also investigated. The biosensor had a linear relation to α-amylase activity and good measurement correlation between 0.66 and 9.83 U/ml. In sample analysis studies, α-amylase activity in baker's yeast was determined by the biosensor.
authors.affiliation: Ege University, Biochemistry Department, Bornova-Izmir, Turkey.
authors.forename: Cagrı
authors.initials: C
authors.lastname: Altug
authors.forename: Umut
authors.initials: U
authors.lastname: Mengulluoglu
authors.forename: Elif
authors.initials: E
authors.lastname: Kurt
authors.forename: Secil
authors.initials: S
authors.lastname: Kaya
authors.forename: Erhan
authors.initials: E
authors.lastname: Dinckaya
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D002623:Chemistry Techniques, Analytical; D003432:Cross-Linking Reagents / Q000737:chemistry; D004563:Electrochemistry; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005780:Gelatin / Q000737:chemistry; D005947:Glucose / Q000378:metabolism; D005949:Glucose Oxidase / Q000378:metabolism*; D005976:Glutaral / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D010100:Oxygen / Q000032:analysis* / Q000378:metabolism; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013213:Starch / Q000378:metabolism*; D013696:Temperature; D015003:Yeasts / Q000737:chemistry; D000516:alpha-Amylases / Q000378:metabolism*
chemical_list: D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D005780:Gelatin; D013213:Starch; D005949:Glucose Oxidase; D000516:alpha-Amylases; D005947:Glucose; D010100:Oxygen; D005976:Glutaral