title: Strain engineering for improved expression of recombinant proteins in bacteria.
abstract: Protein expression in Escherichia coli represents the most facile approach for the preparation of non-glycosylated proteins for analytical and preparative purposes. So far, the optimization of recombinant expression has largely remained a matter of trial and error and has relied upon varying parameters, such as expression vector, media composition, growth temperature and chaperone co-expression. Recently several new approaches for the genome-scale engineering of E. coli to enhance recombinant protein expression have been developed. These methodologies now enable the generation of optimized E. coli expression strains in a manner analogous to metabolic engineering for the synthesis of low-molecular-weight compounds. In this review, we provide an overview of strain engineering approaches useful for enhancing the expression of hard-to-produce proteins, including heterologous membrane proteins.
authors.affiliation: Department of Chemical Engineering, The University of Texas at Austin, 78712, USA.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Makino
authors.forename: Georgios
authors.initials: G
authors.lastname: Skretas
authors.forename: George
authors.initials: G
authors.lastname: Georgiou
mesh_terms: D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D005818:Genetic Engineering; D057166:High-Throughput Screening Assays; D019897:Periplasm / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D011994:Recombinant Proteins