title: Isolation of Mycobacterium tuberculosis strains with a silent mutation in rpoB leading to potential misassignment of resistance category.
abstract: Our study provides an alert regarding the transmission of rifampin-susceptible strains of Mycobacterium tuberculosis with a silent substitution in codon 514 of rpoB. Among 1,450 cases, we identified 12 isolates sharing this mutation and related restriction fragment length polymorphism (RFLP) types. The mutation impaired hybridization with the wild-type probes in three independent commercial assays, which could lead to misassignment of resistance.
authors.affiliation: Servicio de Microbiología y Enfermedades Infecciosas, Hospital Gregorio Marañón, Madrid, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Alonso
authors.forename: Juan José
authors.initials: JJ
authors.lastname: Palacios
authors.forename: Marta
authors.initials: M
authors.lastname: Herranz
authors.forename: Ana
authors.initials: A
authors.lastname: Penedo
authors.forename: Angela
authors.initials: A
authors.lastname: Menéndez
authors.forename: Emilio
authors.initials: E
authors.lastname: Bouza
authors.forename: Darío
authors.initials: D
authors.lastname: García de Viedma
mesh_terms: D019943:Amino Acid Substitution / Q000235:genetics; D000995:Antitubercular Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D012321:DNA-Directed RNA Polymerases; D003951:Diagnostic Errors; D024881:Drug Resistance, Bacterial; D005838:Genotype; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D058889:Molecular Typing; D020125:Mutation, Missense; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D009693:Nucleic Acid Hybridization / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D012293:Rifampin / Q000494:pharmacology*
chemical_list: D000995:Antitubercular Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; C505805:rpoB protein, Mycobacterium tuberculosis; D012321:DNA-Directed RNA Polymerases; D012293:Rifampin