title: [Study of three methods on joint toxicity of diazinon, propoxur and bisphenol A on proliferation of mouse RAW264.7 cell].
abstract: OBJECTIVE
  To investigate possible joint toxic effects of diazinon, propoxur and bisphenol A (BPA) on proliferation of RAW264.7 cells in vitro.
  
  
  METHODS
  Cytotoxicity was assessed by MTT assay. The median inhibiting concentration values (IC50) and 95% confidence interval (CI) of diazinon, propoxur and BPA individually and in mixture (mixed according to ratio of IC50) were established by weighted probit method. The types of toxic interaction of diazinon & BPA and propoxur & BPA were assessed by three methods commonly used for binary mixtures, which were Additional Index Method, Equivalent Effect Curve Method and Logistic Regression Method.
  
  
  RESULTS
  After 24-hour expoxure, the IC50 and 95% CI of diazinon, propoxur and BPA to RAW264.7 cells were 194.1 microg/ml (173.4 microg/ml-217.4 microg/ml), 448.4 mg/L (358.2 microg/ml-573.2 microg/ml) , and 37.5 microg/ml (35.3 microg/ml-39.9 microg/ml), respectively. Those of mixtures of diazinon & BPA and propoxur & BPA were 168.8 microg/ml (160.1 microg/ml-178.2 microg/ml) and 253.4 microg/ml (236.0-273.0 microg/ml). In the interaction assessment, three methods all demonstrated an antagonistic action of diazinon & BPA and an addition action of propoxur & BPA.
  
  
  CONCLUSIONS
  It is concluded that the interaction of diazinon & BPA on proliferation of RAW264.7 cells is antagonistic, and that of propoxur & BPA is additive.
authors.affiliation: Center for Disease Control and Prevention of Guangdong Province, Guangzhou 510300, China. tanxiaohua9619@163.com
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Tan
authors.forename: Xingfen
authors.initials: X
authors.lastname: Yang
authors.forename: Qiong
authors.initials: Q
authors.lastname: Huang
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.forename: Weidong
authors.initials: W
authors.lastname: Hao
authors.forename: Junming
authors.initials: J
authors.lastname: Huang
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds / Q000633:toxicity*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D003976:Diazinon / Q000633:toxicity*; D004357:Drug Synergism; D004781:Environmental Exposure; D020128:Inhibitory Concentration 50; D008264:Macrophages / Q000166:cytology / Q000187:drug effects*; D051379:Mice; D010636:Phenols / Q000633:toxicity*; D001074:Propoxur / Q000633:toxicity*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D003976:Diazinon; D010636:Phenols; D001074:Propoxur; C006780:bisphenol A; D000098826:Bisphenol A Compounds