title: IL-1 polymorphisms in children with peptic symptoms in South China.
abstract: BACKGROUND
  Polymorphisms of IL-1 gene cluster are reported to be associated with histological changes and IL-1β expression in the gastric mucosa in adults, especially in Helicobacter pylori-infected subjects. As H. pylori infecting adults and children own different virulence genotypes, the aim of this study was to investigate whether IL-1 polymorphisms are risk factors in young children in South China.
  
  
  METHODS
  A total of 128 children with peptic symptoms were enrolled in this study. Polymorphisms of IL-1B-511 and IL-1B-31 were identified by dual fluorescence PCR. Variable number of tandem repeat region in IL-1RN was detected by conventional PCR and IL-1β mRNA expression by real-time PCR ddCT assay.
  
  
  RESULTS
  IL-1B-31T and IL-1B-511C were completely linked in this study. Significant differences of IL-1B-511/-31 genotypes were observed among different clinical outcomes (p = .001). The IL-1B-511TT/-31CC was mostly found in the moderate gastritis and the above (severe gastritis or gastric ulcer) groups, with percentage of 60.7%. While no association was observed between IL-1RN genotypes and the gastric mucosal histological changes (p = .128). Also no relationships were found between IL-1 polymorphisms and H. pylori infection or gastric mucosal IL-1β mRNA expression level.
  
  
  CONCLUSIONS
  Children with IL-1B-511TT/-31CC may have a risk to develop relatively severe gastric mucosal histological changes in South China.
authors.affiliation: Department of Laboratories, the Second Affiliated Hospital of GuangZhou Medical University, Guangzhou, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.forename: Fangjin
authors.initials: F
authors.lastname: Wang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhou
authors.forename: Zhiying
authors.initials: Z
authors.lastname: Ou
authors.forename: Hongyun
authors.initials: H
authors.lastname: Jia
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Deng
authors.forename: Yunshao
authors.initials: Y
authors.lastname: He
authors.forename: Xiaoman
authors.initials: X
authors.lastname: Wu
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D005260:Female; D005838:Genotype; D016481:Helicobacter Infections / Q000175:diagnosis / Q000453:epidemiology / Q000235:genetics* / Q000382:microbiology; D016480:Helicobacter pylori / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D006801:Humans; D053583:Interleukin-1beta / Q000235:genetics*; D008297:Male; D010437:Peptic Ulcer / Q000175:diagnosis / Q000453:epidemiology / Q000235:genetics* / Q000382:microbiology; D011110:Polymorphism, Genetic; D012307:Risk Factors; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D053583:Interleukin-1beta; D037521:Virulence Factors