title: PCP can enhance dendritic cells to present the HBsAg peptide.
abstract: OBJECTIVE
  To investigate the influence of paraclorophenol (pCP) on dendritic cells loading and presenting HBsAg from peripheral blood monocytes of healthy volunteers identified as hepatitis B vaccine nonresponders.
  
  
  METHODS
  The density gradient centrifugation was performed to isolate mononuclear cells from 10 hepatitis B vaccine nonresponders. The adherent monocytes were incubated with HBsAg adding rhGM-CSF and rhIL-4 in the presence of absence of pCP for 7 days. Then the supernatant was collected for ELISA assays. The culture medium system without pCP was used as negative control and without pCP or HBsAG was named blank control. the matured DCs were co-incubated with autologous T lymphocytes for 72h and the supernatant was also collected for ELISA assays.
  
  
  RESULTS
  In the presence of pCP, the level of IL-12 in supernate (265.68± 16.21) ng/L was significantly higher than the negative control (168.76±10.01) ng/L (P<0.05) and blank control (87±5.79)ng/L (P<0.05); after co-incubated with autologous T lymphocytes for 3 days, the level of IFN-γ with pCP (773.04±32.73) mg/L was also significantly higher than the negative control (573.59±26.11) mg/L (P<0.05) ans blank control (362.81±24.27)mg/L (P<0.05).
  
  
  CONCLUSIONS
  pCP can effectively enhance the dendritic cells loading and presenting HBsAg from peripheral blood monocytes of healthy volunteers identified as hepatitis B vaccine nonresponders, which also can dramatically increase te autologous T lymphocytes response.7
authors.affiliation: State Key Laboratory of Cancer Biology, Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi'an, China. tangshan@fmmu.edu.cn
authors.forename: Shang-hong
authors.initials: SH
authors.lastname: Tang
authors.forename: Liu-cun
authors.initials: LC
authors.lastname: Gao
authors.forename: Zhi-yong
authors.initials: ZY
authors.lastname: Zhang
authors.forename: Yun-ming
authors.initials: YM
authors.lastname: Li
authors.forename: Qian
authors.initials: Q
authors.lastname: Bi
authors.forename: Cao-jun
authors.initials: CJ
authors.lastname: Song
authors.forename: Gui-tao
authors.initials: GT
authors.lastname: Yang
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D002733:Chlorophenols / Q000494:pharmacology*; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000187:drug effects* / Q000276:immunology* / Q000378:metabolism; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D018664:Interleukin-12 / Q000378:metabolism; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology; D010455:Peptides / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism
chemical_list: D000890:Anti-Infective Agents; D002733:Chlorophenols; D006514:Hepatitis B Surface Antigens; D010455:Peptides; D018664:Interleukin-12; C029107:4-chlorophenol; D007371:Interferon-gamma