title: FTH1 binds to Daxx and inhibits Daxx-mediated cell apoptosis.
abstract: As a highly conserved nuclear protein, death domain-associated protein (Daxx) plays an important role in transcriptional control, carcinogenesis, and resistance to virus infection and so on. In order to further investigate the mechanism of Daxx, the yeast two-hybrid technique was used to screen the intra-cellular proteins interacting with Daxx. And 13 positive colonies and three proteins interacting with Daxx were obtained. One of the candidate proteins was identified as ferritin, heavy polypeptide 1(FTH1). The interaction between Daxx and FTH1 was further supported by GST pull-down and co-immunoprecipitation respectively. Then Daxx was determined to induce apoptosis and FTH1 can inhibit Daxx-mediated apoptosis. Besides, it is found that Daxx mediated apoptosis through the Fas-Daxx-ASK1-JNK1 signaling pathway, while FTH1 can inhibit the activation of JNK signaling pathway. We present evidence to demonstrate the FTH1 and Daxx are able to participate in apoptosis pathway through JNK signal molecule and FTH1 can inhibit this pathway.
authors.affiliation: Department of Clinical Laboratory, the First People's Hospital in Jiangbei District, Chongqing 400020, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.forename: Zhi-Yin
authors.initials: ZY
authors.lastname: Du
authors.forename: Jun-Lin
authors.initials: JL
authors.lastname: He
authors.forename: Xue-Qing
authors.initials: XQ
authors.lastname: Liu
authors.forename: Qiu-Bo
authors.initials: QB
authors.lastname: Yu
authors.forename: Ying-Xiong
authors.initials: YX
authors.lastname: Wang
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D017209:Apoptosis / Q000235:genetics*; D015153:Blotting, Western; D056970:Co-Repressor Proteins; D017931:DNA Primers / Q000235:genetics; D005293:Ferritins / Q000235:genetics / Q000378:metabolism*; D005434:Flow Cytometry; D015723:Gene Library; D006367:HeLa Cells; D006801:Humans; D047468:Immunoprecipitation; D018832:Molecular Chaperones; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D010088:Oxidoreductases; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D015398:Signal Transduction / Q000235:genetics*; D020798:Two-Hybrid System Techniques
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D056970:Co-Repressor Proteins; C106985:DAXX protein, human; D017931:DNA Primers; D018832:Molecular Chaperones; D009687:Nuclear Proteins; D005293:Ferritins; C454461:FTH1 protein, human; D010088:Oxidoreductases