title: Primary study and anti-tumor mechanisms of analog from SC002.
abstract: OBJECTIVE
  To study the anti-tumor mechanisms of ascidiacea analog SC002.
  
  
  METHODS
  After treated the tumor cells with ascidiacea analog SC002, the changes of a serials of tumor associated cell signal molecules, including VEGF (vascular endothelial growth factor), P53, P21 and Bcl-2 et al, were detected by Western blot; further more the expression of serum VEGF in tumor-bearing mice and the activity of Caspase-3, 8, 9 in tumor tissue were investigated by ELISA; at last.
  
  
  RESULTS
  Ascidiacea analog SC002 can obviously induce the expression of P53, while decreasing the expression of Bcl-2 and VEGF, but have on effect on p21 in tumor tissue; ELISA shown that SC002 can also reduce the concentration of serum VEGF in tumor bearing mice, but did not affected the activity of Caspase-3, 8 and 9.
  
  
  CONCLUSIONS
  SC002 have the anti-tumor function through promoting the expression of tumor suppressor gene p53, supress the expression of Bcl-2 and VEGF. This founding will provided an important base theory for it's further use as a novel anti-tumor drugs.
authors.affiliation: Institute of infectious disease, 302 Hospital of PLA, Beijing.
authors.forename: Shu-ping
authors.initials: SP
authors.lastname: Chi
authors.forename: Rong
authors.initials: R
authors.lastname: Gao
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
authors.forename: Li-yue
authors.initials: LY
authors.lastname: You
authors.forename: Hai-min
authors.initials: HM
authors.lastname: Lei
authors.forename: Yun
authors.initials: Y
authors.lastname: Cheng
authors.forename: Jin
authors.initials: J
authors.lastname: Li
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D020169:Caspases / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002511:Cephalosporins / Q000494:pharmacology*; D005260:Female; D056945:Hep G2 Cells; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D014183:Transplantation, Heterologous; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D002511:Cephalosporins; D019253:Proto-Oncogene Proteins c-bcl-2; D016159:Tumor Suppressor Protein p53; D042461:Vascular Endothelial Growth Factor A; D020169:Caspases; C430544:SC002