title: Macrocyclic lactone resistance in Dirofilaria immitis.
abstract: Microfilariae were isolated from a Katrina rescue dog that remained microfilariaemic despite successful adulticidal treatments and repeated treatment with high doses of macrocyclic lactones (MLs). The microfilariae were genotyped at two P-glycoprotein single nucleotide polymorphic sites which had been found to correlate with reduced sensitivity to MLs. The genetic polymorphism (GG-GG), previously found to be associated with insensitivity to MLs in vitro, was present at a frequency of 45.3% in microfilariae that survived repeated treatments with high doses of ML anthelmintics. The data show phenotypic and genotypic evidence of ML resistance in Dirofilaria immitis.
authors.affiliation: Institute of Parasitology, McGill University, 21111 Lakeshore Road, Sainte-Anne-de-Bellevue, Quebec H9X3V9, Canada.
authors.forename: Catherine
authors.initials: C
authors.lastname: Bourguinat
authors.forename: Kathy
authors.initials: K
authors.lastname: Keller
authors.forename: Aron
authors.initials: A
authors.lastname: Bhan
authors.forename: Andrew
authors.initials: A
authors.lastname: Peregrine
authors.forename: Timothy
authors.initials: T
authors.lastname: Geary
authors.forename: Roger
authors.initials: R
authors.lastname: Prichard
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology*; D004183:Dirofilaria immitis / Q000187:drug effects* / Q000235:genetics; D004184:Dirofilariasis / Q000469:parasitology*; D004283:Dog Diseases / Q000469:parasitology; D004285:Dogs; D004351:Drug Resistance / Q000235:genetics*; D047029:Lactams, Macrocyclic / Q000494:pharmacology*; D008297:Male; D011110:Polymorphism, Genetic
chemical_list: D000871:Anthelmintics; D047029:Lactams, Macrocyclic