title: [Effect of tetramethylpyrazine on JAK-STAT signal transduction in cardiomyocyte hypertrophy].
abstract: OBJECTIVE
  To study the effect of Tetramethylpyrazine (TMZ), the extracts of Chinese Herbs which accelerat blood circulation, on JAK-STAT signal transduction in cardiomyocyte hypertrophy.
  
  
  METHODS
  Cardiomyocyte hypertrophy Wistar rats model was established. The cardiomyocytes were seperated from one-day-old neonatal rats. The total RNA of cardiomyocytes was extracted by TRIzol reagent. Then the ANP mRNA were detected by RT-PCR. pJAK2, pJAK1 and pSTAT3 molecules were analysed by Western blot respectively.
  
  
  RESULTS
  Statistics analysis showed that TMZ significantly decreased the expression of ANP mRNA in cardiomyocytes (P<0.01), and that TMZ also decreased the levels of pJAK2, pJAK1 or pSTAT3 (P<0.01).
  
  
  CONCLUSIONS
  TMZ has the inhibitory effect on JAK-STAT signal transduction in cardiomyocyte hyertrophy, suggesting the clinical application of traditional Chinese medicine on cardiomyocyte hypertrophy treatment.
authors.affiliation: Department of Immunology, Medical College, Qingdao University, Qingdao 266000, China. meihuagao@yahoo.com.cn
authors.forename: Mei-hua
authors.initials: MH
authors.lastname: Gao
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Bing
authors.initials: B
authors.lastname: Li
authors.forename: Shu-rong
authors.initials: SR
authors.lastname: Ren
authors.forename: Bei
authors.initials: B
authors.lastname: Zhang
mesh_terms: D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D006984:Hypertrophy / Q000139:chemically induced / Q000378:metabolism*; D053612:Janus Kinases / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000378:metabolism*; D011719:Pyrazines / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D050791:STAT Transcription Factors / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D011719:Pyrazines; D050791:STAT Transcription Factors; D000804:Angiotensin II; D053612:Janus Kinases; C017953:tetramethylpyrazine