title: Reconstruction of stratum corneum in organotypically cultured canine keratinocyte-derived CPEK cells.
abstract: The stratum corneum of epidermis is an essential barrier against the external environment and water loss. This study aimed to develop an organotypic culture model that targets the reconstruction of the stratum corneum using canine keratinocyte-derived CPEK cells. The CPEK cells cultured at the air-liquid interface became stratified and formed a stratum corneum-like layer on stratum spinosum- and stratum granulosum-like layers. The CPEK cells in the stratum granulosum-like layer expressed the cornified cell envelope (CCE)-related proteins loricrin and keratinocyte differentiation-associated protein. Organotypically cultured CPEK cells were considered to form a CCE at the stratum granulosum-like layer, allowing the formation of a stratum corneum-like layer. The organotypic culture of CPEK cells could be useful for studying the barrier function of canine stratum corneum.
authors.affiliation: Department of Veterinary Clinical Pathology, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino-shi, Tokyo, 180-8602, Japan.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Yagihara
authors.forename: Toshiki
authors.initials: T
authors.lastname: Okumura
authors.forename: Eri
authors.initials: E
authors.lastname: Shiomi
authors.forename: Nao
authors.initials: N
authors.lastname: Shinozaki
authors.forename: Shiori
authors.initials: S
authors.lastname: Kuroki
authors.forename: Yu
authors.initials: Y
authors.lastname: Sasaki
authors.forename: Keita
authors.initials: K
authors.lastname: Ito
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Ono
authors.forename: Tsukimi
authors.initials: T
authors.lastname: Washizu
authors.forename: Makoto
authors.initials: M
authors.lastname: Bonkobara
mesh_terms: D000818:Animals; D016282:Aspartic Acid Endopeptidases / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D004285:Dogs; D000078404:Epidermal Cells; D004817:Epidermis / Q000378:metabolism*; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism*; D007633:Keratins / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism
chemical_list: D008565:Membrane Proteins; C064554:loricrin; D007633:Keratins; D016282:Aspartic Acid Endopeptidases