title: Suppression of gliadins results in altered protein body morphology in wheat.
abstract: Wheat gluten proteins, gliadins and glutenins, are of great importance in determining the unique biomechanical properties of wheat. Studies have therefore been carried out to determine their pathways and mechanisms of synthesis, folding, and deposition in protein bodies. In the present work, a set of transgenic wheat lines has been studied with strongly suppressed levels of γ-gliadins and/or all groups of gliadins, using light and fluorescence microscopy combined with immunodetection using specific antibodies for γ-gliadins and HMW glutenin subunits. These lines represent a unique material to study the formation and fusion of protein bodies in developing seeds of wheat. Higher amounts of HMW subunits were present in most of the transgenic lines but only the lines with suppression of all gliadins showed differences in the formation and fusion of the protein bodies. Large rounded protein bodies were found in the wild-type lines and the transgenic lines with reduced levels of γ-gliadins, while the lines with all gliadins down-regulated had protein bodies of irregular shape and irregular formation. The size and number of inclusions, which have been reported to contain triticins, were also higher in the protein bodies in the lines with all the gliadins down-regulated. Changes in the protein composition and PB morphology reported in the transgenic lines with all gliadins down-regulated did not result in marked changes in the total protein content or instability of the different fractions.
authors.affiliation: Instituto de Agricultura Sostenible, CSIC, E-14080 Córdoba, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Gil-Humanes
authors.forename: Fernando
authors.initials: F
authors.lastname: Pistón
authors.forename: Peter R
authors.initials: PR
authors.lastname: Shewry
authors.forename: Paola
authors.initials: P
authors.lastname: Tosi
authors.forename: Francisco
authors.initials: F
authors.lastname: Barro
mesh_terms: D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D005903:Gliadin / Q000378:metabolism*; D005983:Glutens / Q000378:metabolism; D008970:Molecular Weight; D030821:Plants, Genetically Modified; D021122:Protein Subunits / Q000378:metabolism; D012639:Seeds / Q000166:cytology / Q000378:metabolism*; D013194:Staining and Labeling; D014908:Triticum / Q000166:cytology / Q000235:genetics / Q000378:metabolism*
chemical_list: D021122:Protein Subunits; D005983:Glutens; D005903:Gliadin; C037302:glutenin