title: Effect of age on surface molecules and cytokine expression in human dendritic cells.
abstract: Dendritic cells (DCs) are central in regulating both innate and acquired immunity, but their possible age-related functional modifications are still unclear. Here we have analyzed the effect of age on LPS-treated monocyte-derived DCs (MDDCs). A negative correlation between age and cell expression of ICAM-1, CD25 and IL-10 was observed in a group of healthy donors. This has been confirmed by a significantly reduced expression of the same molecules in cells of subgrouped elderly versus younger individuals. On the contrary, a positive correlation between age and cell expression of IL-6 and IL-18 has been reported in all the subjects and further supported by a significant increase of the two pro-inflammatory cytokines in cells of elderly versus young subjects. Our data indicate that aging can impair the expression of ICAM-1 and CD25 and skew the production of cytokines, including IL-18, which concur to make a pro-inflammatory milieu. Altogether, the present results point to additional molecules whose role might be relevant in immunosenescence of human DCs, confirming that these cells undergo functional changes during aging.
authors.affiliation: Department of Clinical and Behavioral Neurology, IRCCS Santa Lucia Foundation, Via Ardeatina 306, 00179 Rome, Italy. a.ciaramella@hsantalucia.it
authors.forename: Antonio
authors.initials: A
authors.lastname: Ciaramella
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Spalletta
authors.forename: Federica
authors.initials: F
authors.lastname: Bizzoni
authors.forename: Francesca
authors.initials: F
authors.lastname: Salani
authors.forename: Carlo
authors.initials: C
authors.lastname: Caltagirone
authors.forename: Paola
authors.initials: P
authors.lastname: Bossù
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000276:immunology* / Q000378:metabolism*; D015703:Antigens, CD / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects / Q000276:immunology; D016207:Cytokines / Q000276:immunology / Q000378:metabolism*; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D005260:Female; D006684:HLA-DR Antigens / Q000378:metabolism; D015395:Histocompatibility Antigens Class I / Q000378:metabolism; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D020382:Interleukin-18 / Q000378:metabolism; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D008875:Middle Aged; D009000:Monocytes / Q000166:cytology / Q000187:drug effects; D055815:Young Adult
chemical_list: D015703:Antigens, CD; D016207:Cytokines; D006684:HLA-DR Antigens; D015395:Histocompatibility Antigens Class I; C508609:IL10 protein, human; C508017:IL2RA protein, human; C508600:IL6 protein, human; D020382:Interleukin-18; D053645:Interleukin-2 Receptor alpha Subunit; D015850:Interleukin-6; D008070:Lipopolysaccharides; D008565:Membrane Proteins; D018799:Intercellular Adhesion Molecule-1; D016753:Interleukin-10