title: Dynamics of bacterial communities in cockles (Cerastoderma edule) with respect to trematode parasite (Bucephalus minimus) infestation.
abstract: The bacterial communities associated with the cockle (Cerastoderma edule) were investigated at the individual level through a 10-month monitoring programme. Temporal changes and those changes associated with a common parasite of the cockle, Bucephalus minimus, were investigated by monthly sampling of individuals, selected based on their shell length (cohort monitoring). Cockle bacterial community abundance (CBCA) and diversity (CBCD) were estimated by epifluorescence microscopy counts and automated ribosomal intergenic spacer analysis, respectively. CBCA showed a temporal pattern peaking at 30 × 10(6) cells per gram of cockle flesh and intervalval liquid in October and a significant 1.8-fold increase linked with B. minimus occurrence. CBCD was characterized by 112 ± 26 intergenic transcribed spacer (ITS) per individual and showed a relative homology between individuals (52 ± 6%, Jaccard similarity) in spite of more than 30% of rare ITS. Consistent with an undisturbed evolution of the condition index of the studied cohort individuals as an estimate of their physiological state, neither temporal nor parasite-induced change in CBCA has been related to marked changes in CBCD.
authors.affiliation: Université de Bordeaux UMR 5805 EPOC, Station Marine d'Arcachon, 2 rue du Pr Jolyet, 33120 Arcachon, France. g.meisterhans@epoc.u-bordeaux1.fr
authors.forename: Guillaume
authors.initials: G
authors.lastname: Meisterhans
authors.forename: Natalie
authors.initials: N
authors.lastname: Raymond
authors.forename: Solène
authors.initials: S
authors.lastname: Lebreton
authors.forename: Franck
authors.initials: F
authors.lastname: Salin
authors.forename: Line
authors.initials: L
authors.lastname: Bourasseau
authors.forename: Xavier
authors.initials: X
authors.lastname: de Montaudouin
authors.forename: Frédéric
authors.initials: F
authors.lastname: Garabetian
authors.forename: Florence
authors.initials: F
authors.lastname: Jude-Lemeilleur
mesh_terms: D000818:Animals; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification*; D044822:Biodiversity; D049873:Cardiidae / Q000382:microbiology* / Q000469:parasitology*; D015169:Colony Count, Microbial; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D006790:Host-Parasite Interactions; D014200:Trematoda / Q000472:pathogenicity*
chemical_list: D004269:DNA, Bacterial; D021903:DNA, Ribosomal Spacer