title: Secondary structural analyses of ITS1 in Paramecium.
abstract: The nuclear ribosomal RNA gene operon is interrupted by internal transcribed spacer (ITS) 1 and ITS2. Although the secondary structure of ITS2 has been widely investigated, less is known about ITS1 and its structure. In this study, the secondary structure of ITS1 sequences for Paramecium and other ciliates was predicted. Each Paramecium ITS1 forms an open loop with three helices, A through C. Helix B was highly conserved among Paramecium, and similar helices were found in other ciliates. A phylogenetic analysis using the ITS1 sequences showed high-resolution, implying that ITS1 is a good tool for species-level analyses.
authors.affiliation: Department of Biomedical Science, College of Life Sciences, Ritsumeikan University, Noji Higashi 1–1–1, Kusatsu,Shiga 525–8577, Japan. wwhoseena@hotmail.com
authors.forename: Ryo
authors.initials: R
authors.lastname: Hoshina
mesh_terms: D001483:Base Sequence; D016054:DNA, Protozoan / Q000737:chemistry* / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010247:Paramecium / Q000737:chemistry / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny
chemical_list: D016054:DNA, Protozoan; D021903:DNA, Ribosomal Spacer