title: Coupling non-destructive DNA extraction and voucher retrieval for small soft-bodied Arthropods in a high-throughput context: the example of Collembola.
abstract: Here, we describe a simple method adapted for high-throughput protocols allowing voucher specimen recovery for Collembola and by extension for other soft-bodied small arthropods. A standard extraction protocol was tested to examine the effects of lysis duration (1, 2, 4, 12 h) on DNA concentration, amplification success and specimen condition. Good quality DNA was obtained after 1 h of lysis, while voucher condition was fine for up to 12 h. The lysis step substantially shortens the clearing process necessary for morphological examination.
authors.affiliation: Canadian Center of DNA Barcoding, University of Guelph, 50 Stone Road East, N1G 2W1, Guelph, Ontario, Canada UMR 5202 CNRS "Origine, Structure et Evolution de la Biodiversité", Muséum National d'Histoire Naturelle, C.P.50 45 rue Buffon, 75005 Paris, France.
authors.forename: David
authors.initials: D
authors.lastname: Porco
authors.forename: Rodolphe
authors.initials: R
authors.lastname: Rougerie
authors.forename: Louis
authors.initials: L
authors.lastname: Deharveng
authors.forename: Paul
authors.initials: P
authors.lastname: Hebert