title: [The development and testing of a molecular biological test system for DNA detection of anthrax pathogen by real-time polymerase chain reaction assay].
abstract: The paper presents the results of the development and testing of a molecular biological test system for DNA detection of anthrax pathogen (Bacillus anthracis) by real-time polymerase chain reaction assay. The test system has shown high sensitivity, specificity, and reproducibility of results of analysis, as exemplified by aqueous suspensions of daily agar cultures of Bacillus anthracis strains, related and heterologous species of microorganisms, and clinical materials of experimental animals. There is evidence for the persistence of the basic characteristics of the test system when stored at 22 +/- 2 degrees C for 12 months.
authors.forename: D D
authors.initials: DD
authors.lastname: Abramov
authors.forename: A A
authors.initials: AA
authors.lastname: Vorob'ev
authors.forename: A V
authors.initials: AV
authors.lastname: Kuznetsovskiĭ
authors.forename: A V
authors.initials: AV
authors.lastname: Savinykh
authors.forename: N V
authors.initials: NV
authors.lastname: Onuchina
authors.forename: I V
authors.initials: IV
authors.lastname: Darmov
authors.forename: D Iu
authors.initials: DIu
authors.lastname: Trofimov
authors.forename: S L
authors.initials: SL
authors.lastname: Kuznetsov
mesh_terms: D000818:Animals; D000881:Anthrax / Q000175:diagnosis*; D000942:Antigens, Bacterial / Q000235:genetics; D001408:Bacillus anthracis / Q000235:genetics / Q000302:isolation & purification*; D001427:Bacterial Toxins / Q000235:genetics; D006224:Cricetinae; D004269:DNA, Bacterial / Q000032:analysis*; D006801:Humans; D008647:Mesocricetus; D016133:Polymerase Chain Reaction / Q000379:methods*; D013997:Time Factors
chemical_list: D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D004269:DNA, Bacterial; C030325:anthrax toxin