title: Isolation and characterization of microsatellites in the bird-pollinated, autohexaploid, Eremophila glabra ssp. glabra (R.Br. (Ostenf.)) (Myoporaceae), an Australian endemic plant.
abstract: Thirty-eight microsatellite loci were developed for the bird pollinated, autohexaploid, Eremophila glabra ssp. glabra. A genomic library was screened with dinucleotide and trinucleotide sequence repeats. Polymorphism ranged from one to 21 alleles per locus. Twenty-four loci exhibited null alleles, based on patterns of inheritance between maternal and progeny phenotypes. Cross-species amplification of nine Eremophila species was successful for most primers, indicating wide applicability across the genus. These microsatellites will be used to study the gene flow patterns of fragmented populations of E. glabra ssp. glabra.
authors.affiliation: CSIRO Plant Industry, GPO Box 1600, Canberra, ACT 2601, Australia; Australian National University, The Fenner School of Environment and Society, Canberra, ACT 0200, Australia. carole.elliott@csiro.au
authors.forename: C P
authors.initials: CP
authors.lastname: Elliott