title: miR-29c regulates BACE1 protein expression.
abstract: Recently, BACE1 expression was shown to be regulated by microRNAs, small endogenous RNA molecules that regulate protein expression through sequence-specific interaction with messenger RNA. Here, we showed that microRNA-29c (miR-29c), a miRNA that is enriched in the brain and highly expressed in the APPswe/PSΔE9 mouse lowers BACE1 protein in vitro and in transgenic miR-29c mice. In silico analysis identified two putative target sites in the BACE1 mRNA for the miR-29c family. We chose SH-SY5Y, HEK-293T cell lines and miR-29c transgenic mice for these studies to validate our hypothesis. Significantly, over-expression of miR-29c in SH-SY5Y, HEK-293T cell lines and miR-29c transgenic mice downregulated BACE1 protein levels. Our findings suggest that miR-29c may be an endogenous regulator of BACE1 protein expression.
authors.affiliation: Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS) & Comparative Medicine Center, Peking Union Medical Collage (PUMC), No. 5, Panjiayuan, Nanli, Chaoyang District, Beijing 10021, PR China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Zong
authors.forename: Hailin
authors.initials: H
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Dong
authors.forename: XiongZhi
authors.initials: X
authors.lastname: Quan
authors.forename: Hua
authors.initials: H
authors.lastname: Zhu
authors.forename: YanFeng
authors.initials: Y
authors.lastname: Xu
authors.forename: Lan
authors.initials: L
authors.lastname: Huang
authors.forename: Chunmei
authors.initials: C
authors.lastname: Ma
authors.forename: Chuan
authors.initials: C
authors.lastname: Qin
mesh_terms: D053829:Amyloid Precursor Protein Secretases / Q000037:antagonists & inhibitors* / Q000235:genetics*; D000818:Animals; D016282:Aspartic Acid Endopeptidases / Q000037:antagonists & inhibitors* / Q000235:genetics*; D045744:Cell Line, Tumor; D015536:Down-Regulation / Q000235:genetics*; D005786:Gene Expression Regulation / Q000235:genetics; D057809:HEK293 Cells; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D035683:MicroRNAs / Q000502:physiology*; D009154:Mutation / Q000235:genetics; D019076:Transgenes / Q000235:genetics
chemical_list: C532045:MIRN29a microRNA, human; D035683:MicroRNAs; D053829:Amyloid Precursor Protein Secretases; D016282:Aspartic Acid Endopeptidases; C509280:BACE1 protein, human