title: Oxygenated perfluorocarbons protect the intestine from the ischemia/reperfusion injury in rabbits.
abstract: OBJECTIVE
  To investigate whether intraluminal administration of oxygenated perfluorocarbons (PFCs) protects the enterocyte from acute ischemia-reperfusion (I/R) injury.
  
  
  METHODS
  Twenty rabbits were divided in 4 groups: sham-operated controls (group A), acute I/R (group B), acute I/R plus infusion of oxygenated PFCs 30 minutes before ischemia (group C), and acute I/R plus infusion of oxygenated PFCs 30 minutes before reperfusion (group D). Serum creatine phosphokinase (CPK) and mucosal disaccharidase activity were examined. Intestinal biopsies were obtained for electron microscopy study.
  
  
  RESULTS
  Group B CPK mean values are 3495.2 ± 157.35 and 4855 ± 350.21 U/L. Group C: 2674.6 ± 265.87 and 3231 ± 232.30. Group D: 2382.2 ± 102.90 and 3217.6 ± 185.61 at 120 and 180 minutes (P<.05). At 180 minutes, maltase and sucrose values were 33.63, 51.88, 8.45, and 19.91, and 17.99, 22.87, 6.62, and 14.24 µmol/min per g for groups A, B, C, and D, respectively (P<.05). Histopathology showed the least cellular deterioration in PFC groups.
  
  
  CONCLUSIONS
  Oxygenated PFCs protect the enterocyte during bowel I/R.
authors.affiliation: 5th Department of Surgery, Hippokration Hospital, Aristotle University of Thessaloniki, Thessaloniki, Greece. achippo@hotmail.com
authors.forename: Achilleas
authors.initials: A
authors.lastname: Ntinas
authors.forename: Dionisios
authors.initials: D
authors.lastname: Vrochides
authors.forename: Stavros
authors.initials: S
authors.lastname: Iliadis
authors.forename: Georgios
authors.initials: G
authors.lastname: Papageorgiou
authors.forename: Athanasia
authors.initials: A
authors.lastname: Alvanou-Achparaki
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Papadimitriou
authors.forename: Charalambos
authors.initials: C
authors.lastname: Spiridis
authors.forename: Thomas
authors.initials: T
authors.lastname: Gerasimidis
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001706:Biopsy; D003402:Creatine Kinase / Q000097:blood; D019610:Cytoprotection; D004195:Disease Models, Animal; D020895:Enterocytes / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure; D005466:Fluorocarbons / Q000494:pharmacology*; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000098:blood supply* / Q000187:drug effects* / Q000648:ultrastructure; D016014:Linear Models; D008854:Microscopy, Electron; D010100:Oxygen / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology*; D011817:Rabbits; D015427:Reperfusion Injury / Q000517:prevention & control*; D013393:Sucrase / Q000378:metabolism; D013997:Time Factors; D000520:alpha-Glucosidases / Q000378:metabolism
chemical_list: D005466:Fluorocarbons; D020011:Protective Agents; D003402:Creatine Kinase; D000520:alpha-Glucosidases; D013393:Sucrase; D010100:Oxygen