title: [Analysis of T-DNA integration pattern in Botrytis cinerea genome].
abstract: OBJECTIVE
  To analyze the T-DNA integration pattern in the genome of grey mold Botrytis cinerea.
  
  
  METHODS
  T-DNA (Transfer DNA) inserted mutant library of Botrytis cinerea was created by Agrobactirium tumfacience mediated transformation. By using TAIL-PCR (Thermal asymmetric interlaced polymerase chain reaction), we amplified and cloned the chromosomal regions flanking T-DNA insertions. The obtained T-DNA flanking sequences were subjected to alignment with standard T-DNA border sequence for identification and analysis of integration.
  
  
  RESULTS
  Up to 69% T-DNA inserted at noncoding regions and 30% inserted at exons. Recombination including deletion or addition of bases in T-DNA region was observed. The right borders of the T-DNA were frequently truncated, and by contrast the left borders were less prone to degradation and appeared to have been inserted in a relatively integrated manner. Extra sequence additions also occurred in T-DNA integration sites.
  
  
  CONCLUSIONS
  Analysis of T-DNA integration pattern in B. cinerea genome will stimulate the functional genomics study of this fungus.
authors.affiliation: College of Biological & Environmental Engineering, Zhejiang University of Technology, Hangzhou 310014, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
authors.forename: Jianyun
authors.initials: J
authors.lastname: Zhang
authors.forename: Tingheng
authors.initials: T
authors.lastname: Zhu
authors.forename: Kun
authors.initials: K
authors.lastname: Wang
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Cui
mesh_terms: D001483:Base Sequence; D020171:Botrytis / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics*; D016680:Genome, Bacterial; D008969:Molecular Sequence Data
chemical_list: D004269:DNA, Bacterial; C036483:T-DNA