title: Possible reduction of hepatoma formation by Smmu 7721 cells in SCID mice and metastasis formation by B16F10 melanoma cells in C57BL/6 mice by Agaricus blazei murill extract.
abstract: Agaricus blazei Murill extract (ABM) has been reported to possess antitumor effects. In this study, the role of ABM in tumor growth and metastasis in vivo was evaluated in experimental Smmu 7721 hepatoma cells in severe combined immunodeficiency (SCID) mice and B16F10 melanoma cells lung metastasis in C57BL/6 mice. For the tumor growth model, the size of the liver tumor mass was about 10 mm to 20 mm in the control group. In comparison with the control group, the tumor mass seem to grow slowly with ABM treatment, especially at the high dose. For the tumor metastasis model, after a six-week treatment, the survival rates of B6 mice were 0%, 30%, 10% and 50% for control group, low, median and high concentration ABM treatment groups, respectively. The survival rate showed that pretreatment of C57BL/6 (B6) mice with ABM lengthened their lifespan after tumor cell inoculation, which supports the notion that ABM successfully reduced lung metastasis formation by B16F10 melanoma cells. The treatment effect was dependent on the concentration of ABM for tumor growth and metastasis in these models.
authors.affiliation: Animal Medicine Center, College of Medicine, National Taiwan University, Taipei, Taiwan, ROC.
authors.forename: Ming-Fang
authors.initials: MF
authors.lastname: Wu
authors.forename: Hsu-Feng
authors.initials: HF
authors.lastname: Lu
authors.forename: Yu-Ming
authors.initials: YM
authors.lastname: Hsu
authors.forename: Ming-Chu
authors.initials: MC
authors.lastname: Tang
authors.forename: Hsueh-Chin
authors.initials: HC
authors.lastname: Chen
authors.forename: Ching-Sung
authors.initials: CS
authors.lastname: Lee
authors.forename: Yi-Yuan
authors.initials: YY
authors.lastname: Yang
authors.forename: Ming-Yang
authors.initials: MY
authors.lastname: Yeh
authors.forename: Hsiung-Kwang
authors.initials: HK
authors.lastname: Chung
authors.forename: Yi-Ping
authors.initials: YP
authors.lastname: Huang
authors.forename: Chih-Chung
authors.initials: CC
authors.lastname: Wu
authors.forename: Jing-Gung
authors.initials: JG
authors.lastname: Chung
mesh_terms: D000364:Agaricus / Q000737:chemistry*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D045424:Complex Mixtures / Q000494:pharmacology*; D004195:Disease Models, Animal; D008175:Lung Neoplasms / Q000401:mortality / Q000473:pathology / Q000556:secondary; D008297:Male; D008545:Melanoma / Q000401:mortality / Q000473:pathology / Q000556:secondary*; D051379:Mice; D008810:Mice, Inbred C57BL; D016513:Mice, SCID; D047368:Tumor Burden / Q000187:drug effects*
chemical_list: D045424:Complex Mixtures