title: Development of duplex RT-PCR-ELISA for the simultaneous detection of hepatitis A virus and hepatitis E virus.
abstract: This study aimed to develop a specific and sensitive duplex reverse transcription polymerase chain reaction enzyme-linked immunosorbent assay (duplex RT-PCR-ELISA) for hepatitis A virus (HAV) and hepatitis E virus (HEV). Duplex RT-PCR-ELISA could detect and differentiate HAV and HEV with specific probes. When ELISA technique was used to detect probe-bound RT-PCR products, duplex RT-PCR-ELISA could detect as little as 0.1 ng/μL HAV and HEV from clinical samples. Human norovirus, enterovirus, poliovirus, murine norovirus and feline calicivirus were used for the specificity test; all were negative. Therefore duplex RT-PCR-ELISA can be used for the simultaneous detection of HAV and HEV in contaminated fecal samples.
authors.affiliation: School of Food Science and Technology, College of Natural Science, Chung-Ang University, Republic of Korea.
authors.forename: Hongmin
authors.initials: H
authors.lastname: Tahk
authors.forename: Min Hwa
authors.initials: MH
authors.lastname: Lee
authors.forename: Kang Bum
authors.initials: KB
authors.lastname: Lee
authors.forename: Doo-Sung
authors.initials: DS
authors.lastname: Cheon
authors.forename: Changsun
authors.initials: C
authors.lastname: Choi
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006506:Hepatitis A / Q000821:virology*; D030041:Hepatitis A virus / Q000235:genetics / Q000302:isolation & purification*; D016751:Hepatitis E / Q000821:virology*; D016752:Hepatitis E virus / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*