title: Oxidative stress response in the opportunistic oral pathogen Fusobacterium nucleatum.
abstract: The anaerobic, Gram-negative bacillus Fusobacterium nucleatum plays a vital role in oral biofilm formation and the development of periodontal disease. The organism plays a central bridging role between early and late colonizers within dental plaque and plays a protective role against reactive oxygen species. Using a two-dimensional gel electrophoresis and mass spectrometry approach, we have annotated 78 proteins within the proteome of F. nucleatum subsp. nucleatum and identified those proteins whose apparent intracellular concentrations change in response to either O(2)- or H(2)O(2)-induced oxidative stress. Three major protein systems were altered in response to oxidative stress: (i) proteins of the alkyl hydroperoxide reductase/thioredoxin reductase system were increased in intracellular concentration; (ii) glycolytic enzymes were modified by oxidation (i.e. D-glyceraldehyde 3-phosphate dehydrogenase, and fructose 6-phosphate aldolase) or increased in intracellular concentration, with an accompanying decrease in ATP production; and (iii) the intracellular concentrations of molecular chaperone proteins and related proteins (i.e. ClpB, DnaK, HtpG, and HrcA) were increased.
authors.affiliation: Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, Nova Scotia, Canada.
authors.forename: Craig H
authors.initials: CH
authors.lastname: Steeves
authors.forename: Joanna
authors.initials: J
authors.lastname: Potrykus
authors.forename: David A
authors.initials: DA
authors.lastname: Barnett
authors.forename: Stephen L
authors.initials: SL
authors.lastname: Bearne
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis / Q000378:metabolism*; D050260:Carbohydrate Metabolism; D018833:Chaperonins / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D016967:Fusobacterium nucleatum / Q000378:metabolism / Q000502:physiology*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D010100:Oxygen / Q000494:pharmacology; D020543:Proteome / Q000187:drug effects / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
chemical_list: D001426:Bacterial Proteins; D020543:Proteome; D006861:Hydrogen Peroxide; D018833:Chaperonins; D010100:Oxygen