title: Type V collagen-induced upregulation of capn2 (large subunit of m-calpain) gene expression and DNA fragmentation in 8701-BC breast cancer cells.
abstract: Type V collagen is known to be over-deposited in the stroma of ductal infiltrating carcinomas of the breast. When used as a substrate, type V collagen restrains growth and invasion, and affects gene expression of 8701-BC ductal infiltrating carcinomas cells. Here we supplement existing data by demonstrating type V collagen dependent upregulation of capn2 gene expression in 8701-BC cells through differential display-PCR and Western blot assays. Furthermore, we suggest that our data obtained by centrifugal sedimentation and electrophoresis strongly suggest a correlation between calpain overproduction and DNA fragmentation, since the incubation with calpain inhibitor partly reverts the latter.
authors.affiliation: Dipartimento di Biologia Cellulare e dello Sviluppo, Università di Palermo, I-90128 Palermo, Italy. claudio.luparello@unipa.it
authors.forename: Claudio
authors.initials: C
authors.lastname: Luparello
authors.forename: Rosalia
authors.initials: R
authors.lastname: Sirchia
mesh_terms: D001943:Breast Neoplasms / Q000201:enzymology* / Q000378:metabolism / Q000473:pathology; D002154:Calpain / Q000235:genetics*; D045744:Cell Line, Tumor; D024062:Collagen Type V / Q000494:pharmacology*; D053938:DNA Fragmentation / Q000187:drug effects*; D004273:DNA, Neoplasm / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction; D015854:Up-Regulation / Q000187:drug effects*
chemical_list: D024062:Collagen Type V; D004273:DNA, Neoplasm; D002154:Calpain; C569870:CAPN2 protein, human