title: Degradation pathway, toxicity and kinetics of 2,4,6-trichlorophenol with different co-substrate by aerobic granules in SBR.
abstract: The present study deals with cultivation of 2,4,6-trichlorophenol (TCP) degrading aerobic granules in two SBR systems based on glucose and acetate as co-substrate. Biodegradation of TCP containing wastewater starting from 10 to 360 mg L(-1) with more than 90% efficiency was achieved. Sludge volume index decreases as the operation proceeds to stabilize at 35 and 30 mL g(-1) while MLVSS increases from 4 to 6.5 and 6.2 g L(-1) for R1 (with glucose as co-substrate) and R2 (with sodium acetate as co-substrate), respectively. FTIR, GC and GC/MS spectral studies shows that the biodegradation occurred via chlorocatechol pathway and the cleavage may be at ortho-position. Haldane model for inhibitory substrate was applied to the system and it was observed that glucose fed granules have a high specific degradation rate and efficiency than acetate fed granules. Genotoxicity studies shows that effluent coming from SBRs was non-toxic.
authors.affiliation: Environmental Research Laboratory, Department of Chemistry, Aligarh Muslim University, Faculty of Science, Aligarh 202 002, UP, India.
authors.forename: Mohammad Zain
authors.initials: MZ
authors.lastname: Khan
authors.forename: Pijush Kanti
authors.initials: PK
authors.lastname: Mondal
authors.forename: Suhail
authors.initials: S
authors.lastname: Sabir
authors.forename: Vinod
authors.initials: V
authors.lastname: Tare
mesh_terms: D000332:Aerobiosis / Q000187:drug effects; D001673:Biodegradation, Environmental / Q000187:drug effects; D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors / Q000382:microbiology*; D002733:Chlorophenols / Q000302:isolation & purification* / Q000633:toxicity*; D008401:Gas Chromatography-Mass Spectrometry; D007700:Kinetics; D009152:Mutagenicity Tests; D010957:Plasmids / Q000378:metabolism; D012722:Sewage / Q000382:microbiology*; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D014835:Volatilization / Q000187:drug effects
chemical_list: D002733:Chlorophenols; D012722:Sewage; C024564:2,4,6-trichlorophenol