title: Effect of dehydrozingerone, a half analog of curcumin on dexamethasone-delayed wound healing in albino rats.
abstract: Oxidative stress is triggered by the wound which results in the production of reactive oxygen species (ROS), thereby delaying normal wound repair. Therefore, it is important to reduce the level of ROS to improve healing. A known antioxidant, dehydrozingerone (DHZ) was synthesized and selected for the study. The authors aimed to investigate the wound healing action of topical (100 mg/wound) and systemic (100 mg/kg, p. o.). DHZ on different wound models in normal and dexamethasone (DEX)-suppressed healing. Topical DHZ showed a significant (P < 0.05) rise in tensile strength when compared to control in normal healing. Significant (P < 0.05) wound closure was observed from 3 to 9 days in DHZ oral and gel treated groups. There was a significant (P < 0.05) rise in hydroxyproline content with the DHZ treated groups when compared to control. Systemic DHZ exhibited a significant (P < 0.05) increase in lysyl oxidase (LO) levels of 3.73 ± 0.15 nmol of H(2)O(2) when compared to control. In DEX-suppressed healing, showed good pro-healing activity with respect to the parameters mentioned above. DHZ treatment exhibited a parabolic dose response of ROS inhibition with a plateau effect at 75 μM. There was a steady and constant increase in the % NO inhibition with increasing doses of DHZ. Oral DHZ is effective in accelerating the healing process in both normal and dexamethasone-suppressed wounds. Our study suggests that DHZ (half analog of curcumin) supplementation reduces the steroid-induced delay in wound healing.
authors.affiliation: Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, 576104 Karnataka, India.
authors.forename: Mallikarjuna C
authors.initials: MC
authors.lastname: Rao
authors.forename: Arun T
authors.initials: AT
authors.lastname: Sudheendra
authors.forename: Pawan G
authors.initials: PG
authors.lastname: Nayak
authors.forename: Piya
authors.initials: P
authors.lastname: Paul
authors.forename: Gopalan N
authors.initials: GN
authors.lastname: Kutty
authors.forename: Rekha R
authors.initials: RR
authors.lastname: Shenoy
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D002460:Cell Line; D006224:Cricetinae; D004247:DNA / Q000378:metabolism; D003907:Dexamethasone / Q000494:pharmacology*; D004355:Drug Stability; D057075:Enzyme Assays; D005782:Gels; D005982:Glutathione Transferase / Q000378:metabolism; D006097:Granulation Tissue / Q000187:drug effects / Q000378:metabolism; D006909:Hydroxyproline / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D008249:Protein-Lysine 6-Oxidase / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D012867:Skin / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D013343:Styrenes / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism; D013718:Tensile Strength / Q000187:drug effects; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000975:Antioxidants; D005782:Gels; D008070:Lipopolysaccharides; D017382:Reactive Oxygen Species; D013343:Styrenes; D009569:Nitric Oxide; D012313:RNA; D003907:Dexamethasone; C052933:methyl-3-methoxy-4-hydroxystyryl ketone; D004247:DNA; D002374:Catalase; D013482:Superoxide Dismutase; D008249:Protein-Lysine 6-Oxidase; D005982:Glutathione Transferase; D006909:Hydroxyproline