title: Screening of ESTs from Mytilus for the detection of SSR markers in Mytilus californianus.
abstract: A set of expressed sequence tag-simple sequence repeat (EST-SSR) markers for the genus Mytilus was developed through bioinformatic mining of the GenBank public database. A total of 33 782 EST sequences from GenBank were downloaded and screened for di-, tri- and tetranucleotide, with 1274 EST containing SSR markers. Nine microsatellite markers were characterized in Mytilus californianus with a number of alleles per locus ranging from two to six, and total observed and expected heterozygosities ranging from 0.490 to 0.730 and from 0.510 to 0.860 respectively. Cross-species amplification was achieved in several other species, confirming the usefulness of these markers in Mytilus genetics.
authors.affiliation: Laboratory of Molecular Ecology, Genomics and Evolutionary Studies, Department of Biology, Faculty of Chemistry and Biology, University of Santiago of Chile, Avda. Libertador Bernardo O'Higgins 3363, Santiago, Chile Instituto de Biología Marina, Universidad Austral de Chile, Casilla 567, Valdivia, Chile Center for Bioinformatics and Molecular Simulations, University of Talca, Chile. 2 Norte 685, Talca, Chile.
authors.forename: R
authors.initials: R
authors.lastname: Vidal
authors.forename: C
authors.initials: C
authors.lastname: Peñaloza
authors.forename: R
authors.initials: R
authors.lastname: Urzúa
authors.forename: J E
authors.initials: JE
authors.lastname: Toro