title: [Simultaneous determination of five yellow dyes in foods by high performance liquid chromatography coupled with tandem mass spectrometry].
abstract: A method based on high performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) was developed for simultaneous determination of five yellow industrial dyes. The separations were performed with an Agilent ODS C18 column at a flow rate of 0.3 mL/min. The mobile phase was 5 mmol/L ammonium acetate (containing 0.1% formic acid)-acetonitrile (3:2, v/v). Under the optimized detection conditions, the linear ranges for Chrysoidine G and Basic Yellow 2 were 5.0-80.0 mg/L, and for Acid Orange I, Acid orange Il and Acid Yellow 36 were 10.0-160.0 microg/L. The limits of quantification for Chrysoidine G, Basic Yellow 2, Acid Orange I, Acid Orange II and Acid Yellow 36 were 20, 20, 40, 40 and 40 ng/g, respectively. The relative standard deviations of reproducibility of this method for retention time and peak area were no more than 0.50% and 2.14%, respectively. The method was applied to determine the recoveries of the above five dyes in chicken, bean products and yellow croaker were between 79.8%-95.2% with satisfactory results.
authors.affiliation: Hangzhou Institution of Quality and Technical Supervision and Inspection, Hangzhou 310019, China.
authors.forename: Saijun
authors.initials: S
authors.lastname: Lin
authors.forename: Haiyun
authors.initials: H
authors.lastname: Tu
authors.forename: Lan
authors.initials: L
authors.lastname: Sun
authors.forename: Hailong
authors.initials: H
authors.lastname: Xiao
authors.forename: Xiangrong
authors.initials: X
authors.lastname: Pan
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Ma
mesh_terms: D001391:Azo Compounds / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004396:Coloring Agents / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D009281:Naphthalenes / Q000032:analysis; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: C456587:Acid Orange II; D001391:Azo Compounds; D004396:Coloring Agents; D009281:Naphthalenes; C005108:metanil yellow