title: Suppression of the rice heterotrimeric G protein β-subunit gene, RGB1, causes dwarfism and browning of internodes and lamina joint regions.
abstract: In the present study, we investigated the function of the heterotrimeric G protein β-subunit (Gβ) gene (RGB1) in rice. RGB1 knock-down lines were generated in the wild type and d1-5, a mutant deficient for the heterotrimeric G protein α-subunit (Gα) gene (RGA1). Both transgenic lines showed browning of the lamina joint regions and nodes that could be attributed to a reduction of RGB1 function, as the abnormality was not observed in d1-5. The RGB1 knock-down lines generated in d1-5 were shorter, suggesting RGB1 to be a positive regulator of cellular proliferation, in addition to RGA1. The number of sterile seeds also increased in both RGB1 knock-down lines. These results suggest that Gβγ and Gα cooperatively function in cellular proliferation and seed fertility. We discuss the potential predominant role of RGB1 in G protein signaling in rice.
authors.affiliation: Department of Bioscience, Fukui Prefectural University, 4-1-1 Matsuoka Kenjyojima, Eiheiji-cho, Yoshida-gun, Fukui 910-1195, Japan.
authors.forename: Yuzuko
authors.initials: Y
authors.lastname: Utsunomiya
authors.forename: Chihiro
authors.initials: C
authors.lastname: Samejima
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Takayanagi
authors.forename: Yuki
authors.initials: Y
authors.lastname: Izawa
authors.forename: Takahisa
authors.initials: T
authors.lastname: Yoshida
authors.forename: Yuka
authors.initials: Y
authors.lastname: Sawada
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Fujisawa
authors.forename: Hisaharu
authors.initials: H
authors.lastname: Kato
authors.forename: Yukimoto
authors.initials: Y
authors.lastname: Iwasaki
mesh_terms: D049109:Cell Proliferation; D018744:DNA, Plant / Q000235:genetics; D035264:Flowers / Q000033:anatomy & histology / Q000235:genetics / Q000502:physiology; D044385:GTP-Binding Protein alpha Subunits / Q000235:genetics / Q000378:metabolism*; D044387:GTP-Binding Protein beta Subunits / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D012275:Oryza / Q000033:anatomy & histology / Q000235:genetics / Q000502:physiology*; D010641:Phenotype; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000033:anatomy & histology / Q000235:genetics / Q000502:physiology; D018547:Plant Stems / Q000033:anatomy & histology / Q000235:genetics / Q000502:physiology; D030821:Plants, Genetically Modified / Q000033:anatomy & histology / Q000235:genetics / Q000502:physiology; D011401:Promoter Regions, Genetic / Q000235:genetics; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics; D036226:Seedlings / Q000033:anatomy & histology / Q000235:genetics / Q000502:physiology; D012639:Seeds / Q000033:anatomy & histology / Q000235:genetics / Q000502:physiology*; D017384:Sequence Deletion; D015398:Signal Transduction / Q000502:physiology
chemical_list: D018744:DNA, Plant; D044385:GTP-Binding Protein alpha Subunits; D044387:GTP-Binding Protein beta Subunits; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant