title: Cys377 residue in NqrF subunit confers Ag(+) sensitivity of Na+-translocating NADH:quinone oxidoreductase from Vibrio harveyi.
abstract: The Na(+)-translocating NADH:quinone oxidoreductase (Na(+)-NQR) is a component of the respiratory chain of various bacteria that generates a redox-driven transmembrane electrochemical Na(+) potential. The Na(+)-NQR activity is known to be specifically inhibited by low concentrations of silver ions. Replacement of the conserved Cys377 residue with alanine in the NqrF subunit of Na(+)-NQR from Vibrio harveyi resulted in resistance of the enzyme to Ag(+) and to other heavy metal ions. Analysis of the catalytic activity also showed that the rate of electron input into the mutant Na(+)-NQR decreased by about 14-fold in comparison to the wild type enzyme, whereas all other properties of (NqrF)C377A Na(+)-NQR including its stability remained unaffected.
authors.affiliation: Department of Molecular Energetics of Microorganisms, Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Russia.
authors.forename: M S
authors.initials: MS
authors.lastname: Fadeeva
authors.forename: Y V
authors.initials: YV
authors.lastname: Bertsova
authors.forename: L
authors.initials: L
authors.lastname: Euro
authors.forename: A V
authors.initials: AV
authors.lastname: Bogachev
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D001692:Biological Transport; D004579:Electron Transport / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D004926:Escherichia coli / Q000201:enzymology; D005420:Flavoproteins / Q000378:metabolism; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000737:chemistry / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D021381:Protein Transport / Q000187:drug effects; D011808:Quinone Reductases / Q000378:metabolism*; D011809:Quinones / Q000378:metabolism; D012834:Silver / Q000494:pharmacology*; D012964:Sodium / Q000378:metabolism; D014734:Vibrio cholerae / Q000201:enzymology*
chemical_list: D001426:Bacterial Proteins; D004791:Enzyme Inhibitors; D005420:Flavoproteins; D011809:Quinones; D012834:Silver; D012964:Sodium; C084142:sodium-translocating NADH-quinone reductase; D016660:NAD(P)H Dehydrogenase (Quinone); D011808:Quinone Reductases