title: Universal oligonucleotide microarray for sub-typing of Influenza A virus.
abstract: A universal microchip was developed for genotyping Influenza A viruses. It contains two sets of oligonucleotide probes allowing viruses to be classified by the subtypes of hemagglutinin (H1-H13, H15, H16) and neuraminidase (N1-N9). Additional sets of probes are used to detect H1N1 swine influenza viruses. Selection of probes was done in two steps. Initially, amino acid sequences specific to each subtype were identified, and then the most specific and representative oligonucleotide probes were selected. Overall, between 19 and 24 probes were used to identify each subtype of hemagglutinin (HA) and neuraminidase (NA). Genotyping included preparation of fluorescently labeled PCR amplicons of influenza virus cDNA and their hybridization to microarrays of specific oligonucleotide probes. Out of 40 samples tested, 36 unambiguously identified HA and NA subtypes of Influenza A virus.
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, Novosibirsk, Russia.
authors.forename: Vladimir A
authors.initials: VA
authors.lastname: Ryabinin
authors.forename: Elena V
authors.initials: EV
authors.lastname: Kostina
authors.forename: Galiya A
authors.initials: GA
authors.lastname: Maksakova
authors.forename: Alexander A
authors.initials: AA
authors.lastname: Neverov
authors.forename: Konstantin M
authors.initials: KM
authors.lastname: Chumakov
authors.forename: Alexander N
authors.initials: AN
authors.lastname: Sinyakov
mesh_terms: D000465:Algorithms; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000378:metabolism; D005821:Genetic Techniques; D005838:Genotype; D006388:Hemagglutinins / Q000235:genetics; D006801:Humans; D009980:Influenza A virus / Q000235:genetics*; D008957:Models, Genetic; D008969:Molecular Sequence Data; D058889:Molecular Typing / Q000379:methods*; D009439:Neuraminidase / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015345:Oligonucleotide Probes; D010802:Phylogeny; D016133:Polymerase Chain Reaction
chemical_list: D018076:DNA, Complementary; D006388:Hemagglutinins; D015345:Oligonucleotide Probes; D009439:Neuraminidase