title: Polarization-controlled differentiation of human neural stem cells using synergistic cues from the patterns of carbon nanotube monolayer coating.
abstract: We report a method for selective growth and structural-polarization-controlled neuronal differentiation of human neural stem cells (hNSCs) into neurons using carbon nanotube network patterns. The CNT patterns provide synergistic cues for the differentiation of hNSCs in physiological solution and an optimal nanotopography at the same time with good biocompatibility. We demonstrated a polarization-controlled neuronal differentiation at the level of individual NSCs. This result should provide a stable and versatile platform for controlling the hNSC growth because CNT patterns are known to be stable in time unlike commonly used organic molecular patterns.
authors.affiliation: Interdisciplinary Program in Nano-Science and Technology, Seoul National University, Seoul 151-747, Korea.
authors.forename: Sung Young
authors.initials: SY
authors.lastname: Park
authors.forename: Dong Shin
authors.initials: DS
authors.lastname: Choi
authors.forename: Hye Jun
authors.initials: HJ
authors.lastname: Jin
authors.forename: Juhun
authors.initials: J
authors.lastname: Park
authors.forename: Kyung-Eun
authors.initials: KE
authors.lastname: Byun
authors.forename: Ki-Bum
authors.initials: KB
authors.lastname: Lee
authors.forename: Seunghun
authors.initials: S
authors.lastname: Hong
mesh_terms: D000327:Adsorption; D001672:Biocompatible Materials / Q000737:chemistry; D002454:Cell Differentiation; D049109:Cell Proliferation; D002470:Cell Survival; D004815:Epidermal Growth Factor / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008855:Microscopy, Electron, Scanning / Q000379:methods; D036103:Nanotechnology / Q000379:methods*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D058953:Neural Stem Cells / Q000166:cytology*; D012117:Resins, Synthetic / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D037742:Nanotubes, Carbon; D012117:Resins, Synthetic; D016222:Fibroblast Growth Factor 2; C029945:polyimide resin; D004815:Epidermal Growth Factor