title: Reduced expression of the DOG1 gene in Arabidopsis mutant seeds lacking the transcript elongation factor TFIIS.
abstract: TFIIS is a transcript elongation factor that facilitates transcription by RNA polymerase II through blocks to elongation. Arabidopsis plants lacking TFIIS are affected in seed dormancy, which represents a block to complete germination under favourable conditions. We have comparatively profiled the transcript levels of seeds of tfIIs mutants and control plants. Among the differentially expressed genes, the DOG1 gene was identified that is a QTL for seed dormancy. The reduced expression of DOG1 in tfIIs seeds was confirmed by quantitative RT-PCR and Northern analyses, suggesting that down-regulation of DOG1 expression is involved in the seed dormancy phenotype of tfIIs mutants.
authors.affiliation: Cell Biology and Plant Biochemistry, Regensburg University, Regensburg, Germany.
authors.forename: Simon A
authors.initials: SA
authors.lastname: Mortensen
authors.forename: Mads
authors.initials: M
authors.lastname: Sønderkær
authors.forename: Carina
authors.initials: C
authors.lastname: Lynggaard
authors.forename: Marion
authors.initials: M
authors.lastname: Grasser
authors.forename: Kåre L
authors.initials: KL
authors.lastname: Nielsen
authors.forename: Klaus D
authors.initials: KD
authors.lastname: Grasser
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000032:analysis / Q000235:genetics*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D057445:Plant Dormancy / Q000235:genetics; D040641:Quantitative Trait Loci; D012639:Seeds / Q000235:genetics; D035602:Transcriptional Elongation Factors / Q000172:deficiency*
chemical_list: D029681:Arabidopsis Proteins; C532384:DOG1 protein, Arabidopsis; D035602:Transcriptional Elongation Factors; C018648:transcription factor S-II