title: [Determination of main degradation products of lignin using reversed-phase high performance liquid chromatography].
abstract: An analytical method using reversed-phase high performance liquid chromatography (RP-HPLC) was developed for the separation and quantitative determination of main degradation products of lignin (4-hydroxybenzoic acid, vanillic acid, syringic acid, 4-hydroxybenzaldehyde, vanillin and syringaldehyde) during the steam exploded pretreatment for corn stovers. The separation was carried out on a C18 column with the mobile phase of acetonitrile-water (containing 1.5% acetic acid) at 30 degrees C at a flow rate of 0.8 mL/min and the detection wavelengths of 254 and 280 nm. Under the optimized conditions, the correlation coefficients of the 6 compounds were between 0.999 9 and 1.000 0. The recoveries of the 6 compounds were all above 96% and the relative standard deviations (n = 6) were less than 2.5%. This method is suitable for the determination of the main degradation products of lignin during the steam exploded pretreatment of lignocellulosics.
authors.affiliation: Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education Nanjing Forestry University, Nanjing 210037, China.
authors.forename: Zhijing
authors.initials: Z
authors.lastname: Jiang
authors.forename: Junjun
authors.initials: J
authors.lastname: Zhu
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.forename: Zhina
authors.initials: Z
authors.lastname: Lian
authors.forename: Shiyuan
authors.initials: S
authors.lastname: Yu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Yong
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D005707:Gallic Acid / Q000031:analogs & derivatives / Q000032:analysis; D006358:Hot Temperature; D008031:Lignin / Q000737:chemistry*; D010226:Parabens / Q000032:analysis*; D014641:Vanillic Acid / Q000032:analysis*
chemical_list: D010226:Parabens; D005707:Gallic Acid; D008031:Lignin; C001945:syringic acid; D014641:Vanillic Acid; C038193:4-hydroxybenzoic acid