title: Altered contribution of RhoA/Rho kinase signaling in contractile activity of myometrium in leptin receptor-deficient mice.
abstract: In late gestation, enhanced myometrial contractility is mediated in part through increased Rho/Rho kinase. Since leptin, which is elevated in pregnancy and obesity, can directly depress myometrial function, we hypothesized that in leptin receptor-deficient mice, myometrial contractility would be greater in late pregnancy due to increased Rho/Rho kinase activity. To test this, we correlated RhoA and Rho kinase expression to contractility in myometrium from nonpregnant (NP) and late-pregnant (P18) heterozygous leptin receptor-deficient mice (db/+) vs. wild-type (WT) mice. In NP mice, KCl-induced contractions were similar between WT and db/+ myometrium. However, the Rho kinase-dependent component of the contractions was greater in db/+ mice, along with an increased expression of Rho kinase. KCl-induced contractions increased in strength in myometrium from P18 WT and db/+ compared with NP. Although the contribution of Rho kinase to contractions was unchanged in P18 WT mice, it was decreased in P18 db/+ mice. The decrease in Rho kinase-dependent contractions in P18 db/+ mice coincided with reduced RhoA and Rho kinase expression relative to NP db/+. Addition of high-fat-induced abnormal glucose utilization prevented changes in Rho kinase function. We conclude that abnormal leptin signaling increases expression and function of Rho kinase to maintain contractile function in NP myometrium and that during pregnancy the contribution of RhoA and Rho kinase expression to myometrial function is reduced despite an increase in myometrial contractility. Thus, other signaling mechanisms appear to compensate when leptin signaling is reduced to maintain contractile function during pregnancy.
authors.affiliation: Iowa City Veterans Affairs Health Care System, 601 Highway 6 West, Iowa City, IA 52246, USA.
authors.forename: Jeremy S
authors.initials: JS
authors.lastname: Harrod
authors.forename: Cara C
authors.initials: CC
authors.lastname: Rada
authors.forename: Stephanie L
authors.initials: SL
authors.lastname: Pierce
authors.forename: Sarah K
authors.initials: SK
authors.lastname: England
authors.forename: Kathryn G
authors.initials: KG
authors.lastname: Lamping
mesh_terms: D000818:Animals; D001835:Body Weight / Q000502:physiology; D016640:Diabetes, Gestational / Q000378:metabolism* / Q000503:physiopathology; D004195:Disease Models, Animal; D005260:Female; D005951:Glucose Tolerance Test; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D009119:Muscle Contraction / Q000502:physiology*; D009215:Myometrium / Q000378:metabolism*; D009765:Obesity / Q000378:metabolism / Q000503:physiopathology; D011247:Pregnancy; D054411:Receptors, Leptin / Q000235:genetics*; D015398:Signal Transduction / Q000502:physiology; D020741:rho GTP-Binding Proteins / Q000378:metabolism*; D054460:rho-Associated Kinases / Q000378:metabolism*; D020742:rhoA GTP-Binding Protein
chemical_list: D054411:Receptors, Leptin; C515447:leptin receptor, mouse; D054460:rho-Associated Kinases; C526380:RhoA protein, mouse; D020741:rho GTP-Binding Proteins; D020742:rhoA GTP-Binding Protein