title: Engineering bioactive surfaces with Fischer carbene complex: protein A on self-assembled monolayer for antibody sensing.
abstract: A Fischer carbene complex was grafted onto self-assembled monolayers (SAMs) on gold or glass by a copper-free "click" reaction. Pendant lysine residues of protein A obtained from Staphylococcus aureus rapidly reacted with the electrophilic metal complex on SAM effecting a covalent attachment of protein A with the surface. The protein A coated surface further led to bioaffinity immobilization of rabbit IgG in an oriented manner, a feature that also permits its purification from rabbit serum. Rabbit IgG could be removed from protein A coated surface by pH adjustment. The regenerated protein A surface was reused three times without loss of activity.
authors.affiliation: Department of Organic Chemistry, Indian Association for the Cultivation of Science, Kolkata-700032, India.
authors.forename: Piyali
authors.initials: P
authors.lastname: Dutta
authors.forename: Sudeshna
authors.initials: S
authors.lastname: Sawoo
authors.forename: Namrata
authors.initials: N
authors.lastname: Ray
authors.forename: Othman
authors.initials: O
authors.lastname: Bouloussa
authors.forename: Amitabha
authors.initials: A
authors.lastname: Sarkar
mesh_terms: D000818:Animals; D057930:Click Chemistry; D005898:Glass / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology* / Q000302:isolation & purification; D008567:Membranes, Artificial; D008697:Methane / Q000031:analogs & derivatives* / Q000737:chemistry; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology*; D010316:Particle Size; D011817:Rabbits; D013205:Staphylococcal Protein A / Q000737:chemistry / Q000276:immunology*; D013211:Staphylococcus aureus / Q000737:chemistry; D013237:Stereoisomerism; D013499:Surface Properties; D014414:Tungsten / Q000737:chemistry
chemical_list: D007074:Immunoglobulin G; D008567:Membranes, Artificial; D009942:Organometallic Compounds; D013205:Staphylococcal Protein A; C030011:carbene; D006046:Gold; D008697:Methane; D014414:Tungsten