title: IFN-gamma secreted by CD103+ dendritic cells leads to IgG generation in the mesenteric lymph node in the absence of vitamin A.
abstract: Although the induction mechanism of secretory IgA has been well studied, that of IgG in the mucosal compartments is not well understood. In this study, vitamin A deficiency was convincingly shown to be associated with increased IgG in serum and intestinal fluid. We found increased numbers of IgG-secreting B cells in the lamina propria of the small intestine and mesenteric lymph node (MLN) of vitamin A-deficient (VAD) mice. Of note, IFN-γ secreted by MLN dendritic cells (DCs) was significantly augmented in VAD mice, unlike control mice, and CD103(+) DCs were the main subsets to secrete IFN-γ. The aberrant increase of IgG in VAD mice can be ascribable to IFN-γ, because IFN-γ(-/-) VAD mice have normal IgG levels and the addition of rIFN-γ increased IgG production by B cells cocultured with MLN DCs from IFN-γ(-/-) VAD mice. Oral feeding of antibiotics resulted in significant reduction of IgG in VAD mice, indicating a critical role for altered commensal bacteria for IgG class-switching recombination in the absence of vitamin A. Collectively, vitamin A deficiency provokes the generation of IFN-γ-secreting CD103(+) DCs, which may be a critical regulator for IgG generation in the MLN.
authors.affiliation: Mucosal Immunology Section, International Vaccine Institute, Seoul, South Korea 151-818.
authors.forename: Jae-Hoon
authors.initials: JH
authors.lastname: Chang
authors.forename: Hye-Ran
authors.initials: HR
authors.lastname: Cha
authors.forename: Sun-Young
authors.initials: SY
authors.lastname: Chang
authors.forename: Hyun-Jeong
authors.initials: HJ
authors.lastname: Ko
authors.forename: Sang-Uk
authors.initials: SU
authors.lastname: Seo
authors.forename: Mi-Na
authors.initials: MN
authors.lastname: Kweon
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000276:immunology; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D007074:Immunoglobulin G / Q000096:biosynthesis*; D039001:Integrin alpha Chains / Q000276:immunology; D007371:Interferon-gamma / Q000276:immunology* / Q000378:metabolism; D007413:Intestinal Mucosa / Q000276:immunology; D008198:Lymph Nodes / Q000276:immunology*; D008643:Mesentery / Q000166:cytology / Q000276:immunology; D051379:Mice; D014802:Vitamin A Deficiency / Q000276:immunology*
chemical_list: D015703:Antigens, CD; D007074:Immunoglobulin G; D039001:Integrin alpha Chains; C091367:alpha E integrins; D007371:Interferon-gamma