title: CCN3-mediated promotion of sulfated proteoglycan synthesis in rat chondrocytes from developing joint heads.
abstract: Chondrocytes forming articular cartilage are embedded in a vast amount of extracellular matrix having physical stiffness and elasticity, properties that support the mechanical load from bones and enable the flexible movement of synovial joints. Unlike chondrocytes that conduct the growth of long bones by forming the growth plate, articular chondrocytes show suppressed cell proliferation, unless these cells are exposed to pathological conditions such as mechanical overload. In the present study, we found that one of the members of the CCN family, CCN3, was significantly expressed in chondrocytes isolated from the epiphyseal head in developing rat synovial joints. Evaluation of the effect of recombinant CCN3 on those chondrocytes revealed that CCN3 promoted proteoglycan synthesis, whereas this factor repressed the proliferation of the same cells. These results suggest a critical role for CCN3 in the regulation of the biological properties of articular chondrocytes.
authors.affiliation: Department of Biochemistry and Molecular Dentistry, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama, 700-8525, Japan.
authors.forename: Danilo
authors.initials: D
authors.lastname: Janune
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kubota
authors.forename: Noureddine
authors.initials: N
authors.lastname: Lazar
authors.forename: Bernard
authors.initials: B
authors.lastname: Perbal
authors.forename: Seiji
authors.initials: S
authors.lastname: Iida
authors.forename: Masaharu
authors.initials: M
authors.lastname: Takigawa