title: Structural insights into transcription complexes.
abstract: Control of transcription allows the regulation of cell activity in response to external stimuli and research in the field has greatly benefited from efforts in structural biology. In this review, based on specific examples from the European SPINE2-COMPLEXES initiative, we illustrate the impact of structural proteomics on our understanding of the molecular basis of gene expression. While most atomic structures were obtained by X-ray crystallography, the impact of solution NMR and cryo-electron microscopy is far from being negligible. Here, we summarize some highlights and illustrate the importance of specific technologies on the structural biology of protein-protein or protein/DNA transcription complexes: structure/function analysis of components the eukaryotic basal and activated transcription machinery with focus on the TFIID and TFIIH multi-subunit complexes as well as transcription regulators such as members of the nuclear hormone receptor families. We also discuss molecular aspects of promoter recognition and epigenetic control of gene expression.
authors.affiliation: EMBL-Grenoble, BP 181, 6 Rue Jules Horowitz, 38042 Grenoble Cedex 9, France.
authors.forename: Imre
authors.initials: I
authors.lastname: Berger
authors.forename: Alexandre G
authors.initials: AG
authors.lastname: Blanco
authors.forename: Rolf
authors.initials: R
authors.lastname: Boelens
authors.forename: Jean
authors.initials: J
authors.lastname: Cavarelli
authors.forename: Miquel
authors.initials: M
authors.lastname: Coll
authors.forename: Gert E
authors.initials: GE
authors.lastname: Folkers
authors.forename: Yan
authors.initials: Y
authors.lastname: Nie
authors.forename: Vivian
authors.initials: V
authors.lastname: Pogenberg
authors.forename: Patrick
authors.initials: P
authors.lastname: Schultz
authors.forename: Matthias
authors.initials: M
authors.lastname: Wilmanns
authors.forename: Dino
authors.initials: D
authors.lastname: Moras
authors.forename: Arnaud
authors.initials: A
authors.lastname: Poterszman
mesh_terms: D000494:Allosteric Regulation; D000818:Animals; D020285:Cryoelectron Microscopy; D018360:Crystallography, X-Ray; D044127:Epigenesis, Genetic; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D046912:Multiprotein Complexes / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011487:Protein Conformation; D018160:Receptors, Cytoplasmic and Nuclear / Q000737:chemistry / Q000378:metabolism; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D046912:Multiprotein Complexes; D018160:Receptors, Cytoplasmic and Nuclear; D014157:Transcription Factors