title: Mutation of ABO gene in Thai blood donors with A3 phenotype.
abstract: The ABO system is the most important of all blood group systems in transfusion practice. The subgroup gives a weak reaction when treated with anti-A or anti-B. The most common subgroup found in Thai blood donors is subgroup A3, which is characterized by mixed-field agglutination when reacted with anti-A and anti-A,B, was caused by mutation in the ABO gene, especially in the exon 7. In the present study mutation in A3 were charactered in exon 7 of the ABO gene in 10 A3 phenotype Thai blood donors from the National Blood Centre, Thai Red Cross Society by PCR amplification and DNA sequencing. Mutations in exon 7 were identified in the A allele of six cases. In four cases, mutations were detected at positions 646T > A, 681G > A, 771C > T and 829G > A. One case showed a double mutations at positions 467C > Tand 745C > T and one case showed a mutation at position 467C > T. Four cases showed wild type exon 7 as A101 allele. These mutations were previously reported in BGMUT database and no novel mutation was identified These data suggest genetic heterogeneity in A3phenotype in Thai blood donors.
authors.affiliation: Department of Clinical Pathology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand. apiwan_7@hotmail.com
authors.forename: Apiwan
authors.initials: A
authors.lastname: Pipatvanichkul
authors.forename: Parichart
authors.initials: P
authors.lastname: Permpikul
authors.forename: Sasijit
authors.initials: S
authors.lastname: Vejbaesya
authors.forename: Wimol
authors.initials: W
authors.lastname: Chinswangwatanakul
mesh_terms: D000017:ABO Blood-Group System / Q000235:genetics*; D000483:Alleles; D044466:Asian People / Q000235:genetics*; D001483:Base Sequence / Q000235:genetics; D001782:Blood Donors; D005091:Exons; D006801:Humans; D007438:Introns / Q000235:genetics; D010641:Phenotype; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D000017:ABO Blood-Group System