title: Quantitative analysis of DNA methylation of imprinted genes in single human blastocysts by pyrosequencing.
abstract: We report the first quantitative assessment of DNA methylation for any gene in the human preimplantation embryo to reveal that imprints exist at KvDMR1, RB1, SNRPN, and GRB10 in the human blastocyst. For comparison, in two human embryonic stem cell lines, imprints were also observed at KvDMR1, SNRPN, GRB10, and other imprinted loci, whereas RB1 and MEG3 were hypermethylated.
authors.affiliation: Division of Reproduction and Early Development, Leeds Institute of Genetics, Health and Therapeutics, University of Leeds, The LIGHT Laboratories, Clarendon Way, Leeds, United Kingdom. j.huntriss@leeds.ac.uk
authors.forename: John
authors.initials: J
authors.lastname: Huntriss
authors.forename: Kathryn
authors.initials: K
authors.lastname: Woodfine
authors.forename: Joanna E
authors.initials: JE
authors.lastname: Huddleston
authors.forename: Adele
authors.initials: A
authors.lastname: Murrell
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: Rutherford
authors.forename: Kay
authors.initials: K
authors.lastname: Elder
authors.forename: Amir Ali
authors.initials: AA
authors.lastname: Khan
authors.forename: Karen
authors.initials: K
authors.lastname: Hemmings
authors.forename: Helen
authors.initials: H
authors.lastname: Picton
mesh_terms: D001755:Blastocyst / Q000502:physiology*; D002460:Cell Line; D019175:DNA Methylation; D046149:Embryo Culture Techniques; D053595:Embryonic Stem Cells / Q000502:physiology*; D051383:GRB10 Adaptor Protein / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D018392:Genomic Imprinting; D006801:Humans; D024642:Potassium Channels, Voltage-Gated / Q000235:genetics; D019836:Preimplantation Diagnosis / Q000379:methods*; D011506:Proteins / Q000235:genetics; D062085:RNA, Long Noncoding; D016160:Retinoblastoma Protein / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000379:methods*; D055517:snRNP Core Proteins / Q000235:genetics
chemical_list: C096358:GRB10 protein, human; C485621:KCNQ1OT1 long non-coding RNA, human; C498907:MEG3 non-coding RNA, human; D024642:Potassium Channels, Voltage-Gated; D011506:Proteins; D062085:RNA, Long Noncoding; D016160:Retinoblastoma Protein; C526417:SNRPN protein, human; D055517:snRNP Core Proteins; D051383:GRB10 Adaptor Protein