title: [Detection of TERC gene amplification by fluorescence in-situ hybridization in cervical intraepithelial lesions].
abstract: OBJECTIVE
  To explore the feasibility and practical value of fluorescence in situ hybridization (FISH) detection of TERC gene amplification in cervical intraepithelial lesions (CIN) and squamous cell carcinoma (SCC).
  
  
  METHODS
  Tissue microarray was constructed to cover 150 cases of various cervical conditions, including 24 cases of normal cervical mucosa, 78 cases of CINs (CINI, 25 cases; CINII, 21 cases and CINIII, 32 cases) and 48 cases of SCC. FISH was used to detect TERC gene amplification.
  
  
  RESULTS
  TERC gene amplification was detected in 8% (2/25) CINI, 47.6% (10/21) CINII, 71.9% (23/32) CINIII and 87.5% (42/48) SCC. There were significant differences among these groups (P < 0.05). The amplification rates of TERC gene in SCC, CINIII and CINII were significantly higher than those of normal cervical epithelium and CINI (P < 0.05). Significant differences were also observed among CINI and CINII, CINIII and SCC (P < 0.05), and between CINII and SCC (P < 0.05). There were no significant differences between normal cervical epithelium and CINI, CINII and CIN III, and between CINIII and SCC (P > 0.05). FISH detection of amplification of TERC gene in CINI and CINII-III demonstrated the following statistics: sensitivity of 62.3%, specificity of 92.0%, accuracy of 71.8%, positive and negative predictive values of 94.3% and 53.5%, respectively.
  
  
  CONCLUSIONS
  FISH detection is a reliable method in detecting TERC gene amplification using paraffin tissue sections. When histological evaluation becomes difficult, TERC amplification detectable by FISH may offer a diagnostic distinction of CINI from CINII. Moreover, TERC amplification may be used as a biomarker in predicting CIN progression to invasive cancer.
authors.affiliation: Department of Pathology, Hebei General Hospital, Shijiazhuang 050051, China.
authors.forename: Yan-long
authors.initials: YL
authors.lastname: YUAN
authors.forename: Chun-nian
authors.initials: CN
authors.lastname: HE
authors.forename: Ming-tang
authors.initials: MT
authors.lastname: XU
authors.forename: Cui-qing
authors.initials: CQ
authors.lastname: XU
authors.forename: Yu-ning
authors.initials: YN
authors.lastname: SUN
authors.forename: Huan-fen
authors.initials: HF
authors.lastname: ZHAO
authors.forename: Chen
authors.initials: C
authors.lastname: CHEN
mesh_terms: D000236:Adenoma / Q000175:diagnosis / Q000235:genetics; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000235:genetics*; D018450:Disease Progression; D005260:Female; D005784:Gene Amplification; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008875:Middle Aged; D012313:RNA / Q000235:genetics*; D012680:Sensitivity and Specificity; D019098:Telomerase / Q000235:genetics*; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis / Q000235:genetics*; D055815:Young Adult; D002578:Uterine Cervical Dysplasia / Q000175:diagnosis / Q000235:genetics*
chemical_list: D014408:Biomarkers, Tumor; C422952:telomerase RNA; D012313:RNA; D019098:Telomerase