title: Proteomics displays cytoskeletal proteins and chaperones involvement in Hedyotis corymbosa-induced photokilling in skin cancer cells.
abstract: Photodynamic therapy was found to be an effective therapy for local malignant tumors. This study demonstrated that 80 μg/ml Hedyotis corymbosa extracts with 0.8 J/cm(2) fluence dose caused M21 skin cancer cell death. Photoactivated H. corymbosa-induced M21 cell death is a typical apoptosis that is accompanied by nuclear condensation, externalization of phosphatidylserine and the changes in protein expression of apoptosis-related proteins, such as Bcl-2 and caspase family members. This study applied 2D electrophoresis to analyse the proteins involved in the photoactivated H. corymbosa-induced M21 cell apoptosis. We found 12 proteins to be markedly changed. According to the results of protein sequence analysis of these altered protein spots, we identified that the expression of cytoskeletal proteins and chaperones were involved in the photoactivated H. corymbosa-induced M21 cell apoptosis. We further demonstrated that photoactivated H. corymbosa caused a significant effect on the cytoskeleton distribution and mitochondrial activity in M21 cells. Based on the above findings, this study characterized the effects and mechanisms of the photoactivated H. corymbosa-induced apoptosis in M21 skin cancer cells.
authors.affiliation: School of Chinese Medicine Resources, China Medical University, Taichung, Taiwan.
authors.forename: Bang-Jau
authors.initials: BJ
authors.lastname: You
authors.forename: Yang-Chang
authors.initials: YC
authors.lastname: Wu
authors.forename: Chi-Yu
authors.initials: CY
authors.lastname: Wu
authors.forename: Bo-Ying
authors.initials: BY
authors.lastname: Bao
authors.forename: Mei-Yu
authors.initials: MY
authors.lastname: Chen
authors.forename: Yu-Hao
authors.initials: YH
authors.lastname: Chang
authors.forename: Hong-Zin
authors.initials: HZ
authors.lastname: Lee
mesh_terms: D008841:Actin Cytoskeleton / Q000187:drug effects / Q000502:physiology; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D020169:Caspases / Q000502:physiology; D045744:Cell Line, Tumor; D045304:Cytochromes c / Q000502:physiology; D003598:Cytoskeletal Proteins / Q000502:physiology*; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D032064:Hedyotis; D006801:Humans; D008545:Melanoma / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D008928:Mitochondria / Q000187:drug effects / Q000502:physiology; D018832:Molecular Chaperones / Q000502:physiology*; D010590:Phalloidine / Q000494:pharmacology; D010778:Photochemotherapy; D040901:Proteomics; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000502:physiology; D012878:Skin Neoplasms / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D051028:bcl-2-Associated X Protein / Q000502:physiology
chemical_list: D003598:Cytoskeletal Proteins; D004365:Drugs, Chinese Herbal; D018832:Molecular Chaperones; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D010590:Phalloidine; D045304:Cytochromes c; D020169:Caspases