title: Frequencies of IL10 SNP genotypes by multiplex PCR-SSP and their association with viral load and CD4 counts in HIV-1-infected Thais.
abstract: BACKGROUND
  Interleukin (IL)-10 is an immunoregulatory cytokine, levels of which can be influenced by single nucleotide polymorphisms (SNPs) in the promoter. Some, but not all previous studies have shown associations of IL10 SNPs with HIV-1 disease progression, using markers such as viral load or CD4 count. There are few data on IL10 SNP frequencies and HIV-1 disease in regions where non-B HIV-1 subtypes predominate.
  
  
  OBJECTIVE
  To determine genotypes, haplotypes, allele frequencies and associations with markers of HIV-1 disease progression of ILO SNPs.
  
  
  METHODS
  A new multiplexed PCR-SSP assay to detect IL10 SNPs at positions -1082, -819 and -592 was developed and used to determine genotypes and haplotypes in 244 HIV-1 CRF01_AE-infected northern Thais having a median time since HIV-1 infection of 2.7 years.
  
  
  RESULTS
  At position -1082 of IL10, AA genotype and A allele were the most common (87.3% and 93.2%, respectively). The -819 CT and -592 CA genotypes were the most prevalent (44.3%), and -819T and -592A were the most prevalent alleles (64.8%). The ATA/ATA was the most common genotype (42.6%) with the most prevalent haplotype of ATA (64.7%). No associations of any of the three ILO SNPs with CD4+ or CD8+ T cell counts or with viral load were found.
  
  
  CONCLUSIONS
  This first report of IL10-1082A, -819T and the IL10-592A allele frequencies in HIV-l-infected Thais shows the highest frequencies in HIV-l-infected persons worldwide. The lack of association of IL10 SNPs with CD4+ T cell count and viral load suggest that other genes may influence these markers in HIV-l-infected Thais.
authors.affiliation: Department of Microbiology, Chiang Mai University, Thailand. doungnapa@rihes.org
authors.forename: Doungnapa
authors.initials: D
authors.lastname: Kingkeow
authors.forename: Janet M
authors.initials: JM
authors.lastname: McNicholl
authors.forename: Niwat
authors.initials: N
authors.lastname: Maneekarn
authors.forename: Jeerang
authors.initials: J
authors.lastname: Wongtrakul
authors.forename: Sineenart
authors.initials: S
authors.lastname: Taechareonkul
authors.forename: Vinai
authors.initials: V
authors.lastname: Suriyanon
authors.forename: Kenrad E
authors.initials: KE
authors.lastname: Nelson
authors.forename: Ann
authors.initials: A
authors.lastname: Duerr
authors.forename: Sanit
authors.initials: S
authors.lastname: Makonkawkeyoon
mesh_terms: D000328:Adult; D018791:CD4 Lymphocyte Count; D016516:CD4-CD8 Ratio; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D015658:HIV Infections / Q000235:genetics / Q000276:immunology / Q000821:virology; D015497:HIV-1 / Q000502:physiology; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D008297:Male; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D013785:Thailand; D019562:Viral Load; D055815:Young Adult
chemical_list: D016753:Interleukin-10