title: Involvement of GDF-9, leptin, and IGF1 receptors associated with adipose tissue transplantation on fertility restoration in obese anovulatory mice.
abstract: The aim was to analyze the effect of adipose tissue transplantation on growth differentiation factor-9 (GDF-9), insulin growth factor 1 receptor (IGF1R), and leptin receptor (LEPR) protein expression in ovaries of obese anovulatory mice. Leptin-deficient female (ob/ob) and wild-type mice were divided into untreated ob/ob mice and gonadal white adipose tissue transplanted ob/ob mice, with evaluation after 7, 15, and 45 days and compared to control wild-type mice. The corporal weight and glycemia levels increased in the obese group concomitant with polymicrocyst formation and abundant estrone, mimicking anovulatory disease. In the treated group after 45 days, glycemia, weight, ovarian size, and number of follicles were decreased and corpora lutea were decreased. The analysis of GDF-9 revealed that, whereas control ovaries presented follicular localization, the obese ovary lacked this protein. On the other hand, obese ovaries showed elevated expression of IGF1R that was normalized after the transplantation. Finally, LEPR was reduced in obese ovaries, and adipose tissue transplantation was efficient in returning it to normal levels. In conclusion, the adipose tissue transplantation, especially after 45 days, seems to stimulate ovulation, supported by the fact that several proteins involved in ovulation returned to basal levels.
authors.affiliation: Department of Gynecology, Federal University of São Paulo, São Paulo, Brazil.
authors.forename: Melquíades
authors.initials: M
authors.lastname: Pereira
authors.forename: Daniela Berguio
authors.initials: DB
authors.lastname: Vidotti
authors.forename: Ricardo Carneiro
authors.initials: RC
authors.lastname: Borra
authors.forename: Manuel De Jesus
authors.initials: Mde J
authors.lastname: Simões
authors.forename: Ismael Dale Cotrim Guerreiro
authors.initials: ID
authors.lastname: Da Silva
authors.forename: Mauro Abi
authors.initials: MA
authors.lastname: Haidar
mesh_terms: D000818:Animals; D000858:Anovulation / Q000209:etiology / Q000517:prevention & control; D003338:Corpus Luteum / Q000378:metabolism / Q000473:pathology; D005260:Female; D005298:Fertility; D055429:Growth Differentiation Factor 9 / Q000378:metabolism*; D050152:Intra-Abdominal Fat / Q000637:transplantation*; D020738:Leptin / Q000235:genetics; D051379:Mice; D018345:Mice, Knockout; D008820:Mice, Obese; D009765:Obesity / Q000150:complications*; D009929:Organ Size; D010053:Ovary / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D010060:Ovulation; D011085:Polycystic Ovary Syndrome / Q000150:complications / Q000378:metabolism / Q000503:physiopathology / Q000628:therapy*; D017526:Receptor, IGF Type 1 / Q000378:metabolism*; D054411:Receptors, Leptin / Q000378:metabolism*; D040521:Subcutaneous Tissue; D016042:Transplantation, Heterotopic
chemical_list: C525898:Gdf9 protein, mouse; D055429:Growth Differentiation Factor 9; D020738:Leptin; D054411:Receptors, Leptin; C515447:leptin receptor, mouse; D017526:Receptor, IGF Type 1