title: Zinc-binding proteins from boar seminal plasma -- isolation, biochemical characteristics and influence on spermatozoa stored at 4°C.
abstract: Affinity chromatography on Chelating Sepharose Fast Flow Gel-Zn(2+) was used for fractionation of boar seminal plasma proteins. Approximately 30% of total boar seminal plasma proteins showed affinity for zinc ions (ZnBP fraction). Native electrophoresis (PAGE) of ZnBP revealed six protein fractions which separated into 27 bands under denaturing conditions (SDS/PAGE). Two-dimensional electrophoresis (2D PAGE) showed 148 polypeptides with isoelectric points mostly in the basic and neutral pH range. The zinc-binding proteins comprise mainly 10-20 kDa polypeptides which are probably members of the spermadhesin family. ZnBP present in the incubation mixture of spermatozoa stored for 1 or 24 h at 4 °C allowed preservation of a higher percentage of cells exhibiting linear motility in comparison to a control sample stored in PBS. Presented results indicate that proteins binding Zn(2+) ions have a shielding effect on the sperm plasma membrane and acrosome of spermatozoa, protecting these structures against consequences of cold shock.
authors.affiliation: University of Warmia and Mazury in Olsztyn, Department of Animal Biochemistry and Biotechnology, Olsztyn, Poland. mmog@uwm.edu.pl
authors.forename: Marzena
authors.initials: M
authors.lastname: Mogielnicka-Brzozowska
authors.forename: Paweł
authors.initials: P
authors.lastname: Wysocki
authors.forename: Jerzy
authors.initials: J
authors.lastname: Strzeżek
authors.forename: Władysław
authors.initials: W
authors.lastname: Kordan
mesh_terms: D000177:Acrosome / Q000378:metabolism / Q000473:pathology; D000818:Animals; D002352:Carrier Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism / Q000473:pathology; D002850:Chromatography, Gel; D008297:Male; D008970:Molecular Weight; D012034:Refrigeration; D012661:Semen / Q000737:chemistry / Q000378:metabolism*; D013081:Sperm Motility; D013094:Spermatozoa / Q000378:metabolism* / Q000473:pathology; D034421:Sus scrofa; D015032:Zinc / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D015032:Zinc