title: Determination of superoxide dismutase and SOD-mimetic activities by a chemical system: Co2/H2O2/lucigenin.
abstract: The bright chemiluminescence has been observed in the system: Co(2+)/hydrogen peroxide/lucigenin. The chemiluminescence intensity was directly proportional to either cobalt, hydrogen peroxide, or lucigenin concentrations. A procedure of determination of superoxide dismutase (SOD) activity by the chemiluminescence method in the cobalt-hydrogen peroxide-lucigenin system at pH 8.5 is suggested. A linear dependence was established between a relative chemiluminescence intensity and SOD concentration in the range of SOD concentrations between 0 and 4.5 nM, c (1/2) = 0.8 nM. The determination of SOD activity was performed in several tissue samples (rat plasma, erythrocyte hemolysate, and liver mitochondria). A technique of tissue sample preparation with the use of thermal inactivation of interfering proteins at 60 °C was used. The method was successfully applied for comparison of the efficiency of SOD mimetics.
authors.affiliation: Faculty of Fundamental Medicine, Lomonosov Moscow State University, Moscow, Russia. t_msu@mail.ru
authors.forename: T V
authors.initials: TV
authors.lastname: Zhidkova
authors.forename: E V
authors.initials: EV
authors.lastname: Proskurnina
authors.forename: E A
authors.initials: EA
authors.lastname: Parfenov
authors.forename: Yu A
authors.initials: YA
authors.lastname: Vladimirov
mesh_terms: D000166:Acridines / Q000378:metabolism*; D000818:Animals; D003035:Cobalt / Q000378:metabolism*; D004912:Erythrocytes / Q000201:enzymology; D006861:Hydrogen Peroxide / Q000378:metabolism*; D057230:Limit of Detection; D049408:Luminescent Agents / Q000378:metabolism*; D008163:Luminescent Measurements / Q000379:methods*; D008297:Male; D008930:Mitochondria, Liver / Q000201:enzymology; D051381:Rats; D013482:Superoxide Dismutase / Q000097:blood / Q000378:metabolism*
chemical_list: D000166:Acridines; D049408:Luminescent Agents; C033472:10,10'-dimethyl-9,9'-biacridinium; D003035:Cobalt; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase