title: Lectin typing of Campylobacter jejuni using a novel quartz crystal microbalance technique.
abstract: Seven Campylobacter jejuni strains were characterised by a lectin typing assay. The typing system was based on a quartz crystal microbalance technique (QCM) with four commercially available lectins (wheat germ agglutinin, Maackia amurensis lectin, Lens culinaris agglutinin, and Concanavalin A), which were chosen for their differing carbohydrate specificities. Initially, the gold surfaces of the quartz crystals were modified with 11-mercaptoundecanoic acid followed by lectin immobilisation using a conventional amine-coupling technique. Bacterial cells were applied for lectin typing without preliminary treatment, and resonant frequency and dissipation responses were recorded. The adhesion of microorganisms on lectin surfaces was confirmed by atomic force microscopy. Scanning was performed in the tapping mode and the presence of bacteria on lectin-coated surfaces was successfully demonstrated. A significant difference in the dissipation response was observed for different C. jejuni strains which made it possible to use this parameter for discriminating between bacterial strains. In summary, the QCM technique proved a powerful tool for the recognition and discrimination of C. jejuni strains. The approach may also prove applicable to strain discrimination of other bacterial species, particularly pathogens.
authors.affiliation: Department of Infectious Diseases and Medical Microbiology, Lund University, Lund, Sweden. maria.yakovleva@gmail.com
authors.forename: Maria E
authors.initials: ME
authors.lastname: Yakovleva
authors.forename: Anthony P
authors.initials: AP
authors.lastname: Moran
authors.forename: Gulnara R
authors.initials: GR
authors.lastname: Safina
authors.forename: Torkel
authors.initials: T
authors.lastname: Wadström
authors.forename: Bengt
authors.initials: B
authors.lastname: Danielsson
mesh_terms: D015373:Bacterial Typing Techniques; D016123:Campylobacter jejuni / Q000145:classification* / Q000378:metabolism; D003208:Concanavalin A / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D055767:Immobilized Proteins / Q000737:chemistry / Q000378:metabolism; D037102:Lectins / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D058525:Quartz Crystal Microbalance Techniques / Q000379:methods*; D013438:Sulfhydryl Compounds / Q000737:chemistry; D014909:Wheat Germ Agglutinins / Q000737:chemistry
chemical_list: C505222:11-mercaptoundecanoic acid; D005227:Fatty Acids; D055767:Immobilized Proteins; D037102:Lectins; D013438:Sulfhydryl Compounds; D014909:Wheat Germ Agglutinins; D003208:Concanavalin A; D006046:Gold