title: Improved preservation of fine structure of deep-sea microorganisms by freeze-substitution after glutaraldehyde fixation.
abstract: A method was proposed for improving preservation of ultrastructures of deep-sea microorganisms by using rapid-freeze freeze-substitution after glutaraldehyde fixation. This method produced clear high-resolution images of cells appearing in their natural state, close to the quality of images obtained by rapidly freezing freeze-substituted specimens of living cells. The method may be useful for observing any microorganism when rapid freezing of living samples is difficult and only glutaraldehyde fixation can be carried out.
authors.affiliation: Medical Mycology Research Center, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8673, Japan. yama@faculty.chiba-u.jp
authors.forename: Masashi
authors.initials: M
authors.lastname: Yamaguchi
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Namiki
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Okada
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Uematsu
authors.forename: Akihiro
authors.initials: A
authors.lastname: Tame
authors.forename: Tadashi
authors.initials: T
authors.lastname: Maruyama
authors.forename: Yoshimichi
authors.initials: Y
authors.lastname: Kozuka
mesh_terms: D000818:Animals; D001419:Bacteria / Q000166:cytology / Q000648:ultrastructure*; D017110:Freeze Substitution / Q000379:methods*; D005976:Glutaral / Q000737:chemistry*; D008854:Microscopy, Electron / Q000379:methods; D011077:Polychaeta / Q000382:microbiology / Q000648:ultrastructure; D012623:Seawater / Q000382:microbiology*; D016707:Tissue Fixation / Q000379:methods*
chemical_list: D005976:Glutaral