title: Effect of low-intensity pulsed ultrasound on MMP-13 and MAPKs signaling pathway in rabbit knee osteoarthritis.
abstract: We evaluated the effect of low-intensity pulsed ultrasound (LIPUS) on MMP-13 and MAPKs expression in rabbit knee osteoarthritis (OA). For this purpose, 18 New Zealand white rabbits were randomly and equally divided into O + L, O - L, and SO groups. In O + L group, animals underwent right back leg ACLT operation and LIPUS radiation. In O - L group, animals underwent ACLT but no LIPUS treatment. In SO (control) group, animals underwent sham operation without LIPUS. After 6 weeks, we assessed the pathologic changes in the articular surface of femoral condyle and compared using Mankin scores. Also, expression of type-II collagen, MMP-13, ERK1/2, p38, and JNK was measured by Western blot. Compared with controls, Mankin scores were higher in O + L (P < 0.05)/O - L (P < 0.01) groups. Compared with O + L group, score was higher in O - L group (P < 0.05). Compared with controls, type-II collagen expression was less in O + L/O - L groups, with more significant decrease in O - L group (P < 0.05). Contrarily, expression of MMP-13, p-ERK1/2, and p-p38 was enhanced in O + L/O - L groups as compared with controls, with more significant increase in O - L group (P < 0.01). Compared with O + L group, expression was higher in O - L group (P < 0.05). We, therefore, concluded that LIPUS application promoted cartilage repair in OA through the downregulation of MMP-13, ERK1/2, and p38.
authors.affiliation: Department of Rehabilitation, Nanjing First Hospital Affiliated to Nanjing Medical University, Nanjing, China.
authors.forename: Xueping
authors.initials: X
authors.lastname: Li
authors.forename: Jianan
authors.initials: J
authors.lastname: Li
authors.forename: Kai
authors.initials: K
authors.lastname: Cheng
authors.forename: Qiang
authors.initials: Q
authors.lastname: Lin
authors.forename: Daxin
authors.initials: D
authors.lastname: Wang
authors.forename: Hongfei
authors.initials: H
authors.lastname: Zhang
authors.forename: Hengyuan
authors.initials: H
authors.lastname: An
authors.forename: Mingxia
authors.initials: M
authors.lastname: Gao
authors.forename: Anliang
authors.initials: A
authors.lastname: Chen
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000000981:diagnostic imaging / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D019902:Chondrocytes / Q000000981:diagnostic imaging / Q000201:enzymology / Q000378:metabolism; D004789:Enzyme Activation; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D020935:MAP Kinase Signaling System; D008297:Male; D053509:Matrix Metalloproteinase 13 / Q000378:metabolism*; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D020370:Osteoarthritis, Knee / Q000000981:diagnostic imaging* / Q000201:enzymology* / Q000378:metabolism / Q000473:pathology; D011817:Rabbits; D014463:Ultrasonography
chemical_list: D020928:Mitogen-Activated Protein Kinases; D053509:Matrix Metalloproteinase 13