title: Evaporative preconcentration of fluorescent protein samples in capillary based microplates.
abstract: The preconcentration of analytes is important in biochemical analysis as it offers the ability to detect for trace species, and increase signal-to-noise ratios when using optical sensing on fluorophores. A strong advantage of the evaporation technique lies in its ability to operate without the need of any energy source; albeit major challenges exist on how to increase the surface area exposure to air for heightened evaporation, ensure no further increases once specified analyte concentrations have been achieved, and not needing any intervening membranes. We demonstrate here that the droplet creation and retraction approach in capillary based microplates offers such abilities whilst at the same time facilitating mixing.
authors.affiliation: Laboratory for Optics, Acoustics, & Mechanics, Department of Mechanical & Aerospace Engineering, Monash University, Clayton, Victoria 3800, Australia.
authors.forename: Fenfen
authors.initials: F
authors.lastname: Shao
authors.forename: Tuck Wah
authors.initials: TW
authors.lastname: Ng
authors.forename: Jonathan Kok Keung
authors.initials: JK
authors.lastname: Lye
authors.forename: Oi Wah
authors.initials: OW
authors.lastname: Liew
mesh_terms: D003890:Desiccation; D005453:Fluorescence; D055616:Microtechnology / Q000295:instrumentation* / Q000379:methods*; D011506:Proteins / Q000032:analysis* / Q000302:isolation & purification*; D013997:Time Factors
chemical_list: D011506:Proteins