title: Cylindrospermopsin inhibits growth and modulates protease activity in the aquatic plants Lemna minor L. and Wolffia arrhiza (L.) Horkel.
abstract: The toxic effects of cylindrospermopsin (cyanobacterial toxin) on animals have been examined extensively, but little research has focused on their effects on plants. In this study cylindrospermopsin (CYN) caused alterations of growth, soluble protein content and protease enzyme activity were studied on two aquatic plants Lemna minor and Wolffia arrhiza in short-term (5 days) experiments. For the treatments we used CYN containing crude extracts of Aphanizomenon ovalisporum (BGSD-423) and purified CYN as well. The maximal inhibitory effects on fresh weight of L. minor and W. arrhiza caused by crude extract were 60% and 54%, respectively, while the maximum inhibitory effects were 30% and 43% in the case of purified CYN at 20 μg ml(-1) CYN content of culture medium. In CYN-treated plants the concentration of soluble protein showed mild increases, especially in W. arrhiza. Protease isoenzyme activity gels showed significant alterations of enzyme activities under the influence of CYN. Several isoenzymes were far more active and new ones appeared in CYN-treated plants. Treatments with cyanobacterial crude extract caused stronger effects than the purified cyanobacterial toxins used in equivalent CYN concentrations.
authors.affiliation: Department of Botany, Faculty of Science and Technology, University of Debrecen, Egyetem tér 1 H-4010 Debrecen, Hungary.
authors.forename: Katalin
authors.initials: K
authors.lastname: Jámbrik
authors.forename: C
authors.initials: C
authors.lastname: Máthé
authors.forename: G
authors.initials: G
authors.lastname: Vasas
authors.forename: I
authors.initials: I
authors.lastname: Bácsi
authors.forename: G
authors.initials: G
authors.lastname: Surányi
authors.forename: S
authors.initials: S
authors.lastname: Gonda
authors.forename: G
authors.initials: G
authors.lastname: Borbély
authors.forename: Márta
authors.initials: M
authors.lastname: M-Hamvas
mesh_terms: D000470:Alkaloids; D046890:Aphanizomenon / Q000737:chemistry*; D059001:Aquatic Organisms / Q000187:drug effects; D029064:Araceae / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development; D001427:Bacterial Toxins; D000087522:Cyanobacteria Toxins; D007527:Isoenzymes / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D014498:Uracil / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000633:toxicity
chemical_list: D000470:Alkaloids; D001427:Bacterial Toxins; D000087522:Cyanobacteria Toxins; D007527:Isoenzymes; D010940:Plant Proteins; C089595:cylindrospermopsin; D014498:Uracil; D010447:Peptide Hydrolases