title: Development and characterization of nine polymorphic microsatellite markers in the Chilean kelp Lessonia nigrescens.
abstract: A total of nine microsatellite loci were isolated and characterized in the Chilean kelp Lessonia nigrescens Bory. Using two different enriched libraries, we observed 1-14 alleles per locus in two samples of 21 kelp individuals each. The observed heterozygosities ranged from 0.05 to 0.80 and all loci are in Hardy-Weinberg equilibrium for one or both samples. Seventeen samples collected from different sites showed high allele diversity along the species distribution. The variation detected at these markers is currently being used for the study of populations of Lessonia nigrescens at different geographical scales.
authors.affiliation: Center for Advanced Studies in Ecology and Biodiversity, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Casilla 114-D, Santiago, Chile.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Faugeron
authors.forename: David
authors.initials: D
authors.lastname: Veliz
authors.forename: Gioconda
authors.initials: G
authors.lastname: Peralta
authors.forename: Javier
authors.initials: J
authors.lastname: Tapia
authors.forename: Florence
authors.initials: F
authors.lastname: Tellier
authors.forename: Claire
authors.initials: C
authors.lastname: Billot
authors.forename: Enrique
authors.initials: E
authors.lastname: Martinez