title: Chondroitinase ABC treatment and the phenotype of neural progenitor cells isolated from injured rat spinal cord.
abstract: The aim of the present study was to investigate whether enzyme chondroitinase ABC (ChABC) treatment influences the phenotype of neural progenitor cells (NPCs) derived from injured rat spinal cord. Adult as well as fetal spinal cords contain a pool of endogenous neural progenitors cells, which play a key role in the neuroregenerative processes following spinal cord injury (SCI) and hold particular promise for therapeutic approaches in CNS injury or neurodegenerative disorders. In our study we used in vitro model to demonstrate the differentiation potential of NPCs isolated from adult rat spinal cord after SCI, treated with ChABC. The intrathecal delivery of ChABC (10 U/ml) was performed at day 1 and 2 after SCI. The present findings indicate that the impact of SCI resulted in a decrease of all NPCs phenotypes and the ChABC treatment, on the contrary, caused an opposite effect.
authors.affiliation: Institute of Neurobiology, CE, Slovak Academy of Sciences, Košice, Slovakia. slovinska@saske.sk
authors.forename: L
authors.initials: L
authors.lastname: Slovinská
authors.forename: I
authors.initials: I
authors.lastname: Novotná
authors.forename: D
authors.initials: D
authors.lastname: Cížková
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D019765:Chondroitin ABC Lyase / Q000494:pharmacology* / Q000627:therapeutic use; D008297:Male; D009474:Neurons / Q000187:drug effects / Q000201:enzymology*; D010641:Phenotype; D051381:Rats; D017208:Rats, Wistar; D013116:Spinal Cord / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D013119:Spinal Cord Injuries / Q000188:drug therapy* / Q000201:enzymology* / Q000473:pathology; D013234:Stem Cells / Q000187:drug effects / Q000201:enzymology*; D016896:Treatment Outcome
chemical_list: D019765:Chondroitin ABC Lyase