title: Intracellular free Fatty-Acid release and lipid-peroxidation in cultured human breast-cancer cells in response to gamma-linolenic Acid with iron (gla + fe).
abstract: Intracellular free fatty acid (FFA) release and peroxidation of polyunsaturated fatty acid (PUFA) were studied in cultured human breast cancer cells (ZR-75-1) exposed to gamma-linolenic acid with iron (GLA + Fe). This treatment results in cell death. Increased intracellular FFA were observed in association with both the accelerated peroxidation of PUFA and the killing effect. Vitamin E reduced all three effects. The FFA were methyl esterified and analyzed by gas chromatography-mass spectrometry. The identified FFAs were 16: 0, 18: 3, 20: 3 and 20: 4 (numbers of carbons and double bonds indicated). These results suggest an association of intracellular FFA release with the peroxidation of PUFA and the cancer cell-killing by GLA in the presence of iron.
authors.affiliation: NATL RES COUNCIL CANADA,ATLANTIC REG LAB,INST MARINE BIOSCI,HALIFAX B3H 3Z1,NS,CANADA.
authors.forename: S
authors.initials: S
authors.lastname: Takeda
authors.forename: P
authors.initials: P
authors.lastname: Sim
authors.forename: D
authors.initials: D
authors.lastname: Horrobin
authors.forename: K
authors.initials: K
authors.lastname: Chisholm
authors.forename: V
authors.initials: V
authors.lastname: Simmons
authors.forename: G
authors.initials: G
authors.lastname: Ells
authors.forename: D
authors.initials: D
authors.lastname: Jenkins
authors.forename: N
authors.initials: N
authors.lastname: Morsefisher