title: Autologous bone marrow mesenchymal stem cells implantation for cartilage defects: two cases report.
abstract: OBJECTIVE
  The authors reported the results of autologous bone marrow mesenchymal stem cells (BM-MSCs) implantation in two patients with large traumatic cartilage defects of the knee.
  
  
  METHODS
  Two patients with grade 3-4 according to the International Cartilage Repair Society Classification System were performed autologous bone marrow mesenchymal stem cells (BM-MSCs) implantation on December 2007 and January 2008. The bone marrow aspiration was performed in the outpatient visit under local anesthesia and sent to the laboratory for BM-MSCs isolation and expansion. The BM-MSCs were re-implanted into the defects with the three-dimensional collagen scaffold. The patients were clinical evaluated preoperatively and postoperatively with Knee and Osteoarthritis Outcome Score (KOOS), International Knee Documentation Committee Score (IKDC Score) and arthroscopic examination. The duration of follow-up was 30-31 months.
  
  
  RESULTS
  There was no postoperative complication. The clinical evaluation with Knee and Osteoarthritis Outcome Score (KOOS) and International Knee Documentation Committee Score (IKDC Score) showed significant improvement. The arthroscopic assessment showed the good defect fill, stiffness and incorporation to the adjacent cartilage.
  
  
  CONCLUSIONS
  The autologous bone marrow mesenchymal stem cells implantation showed the potential for the treatment of large cartilage defects. The one-stage procedure is the advantage over the conventional autologous chondrocytes implantation. The long-term follow-up with long last hyaline-like cartilage is required.
authors.affiliation: Department of Orthopedics, Faculty of Medicine, HRH Princess Maha Chakri Sirindhorn Medical Center, Srinakhrinwirot University, Nakhon Nayok, Thailand. chann@swu.ac.th
authors.forename: Channarong
authors.initials: C
authors.lastname: Kasemkijwattana
authors.forename: Suradej
authors.initials: S
authors.lastname: Hongeng
authors.forename: Suraphol
authors.initials: S
authors.lastname: Kesprayura
authors.forename: Visit
authors.initials: V
authors.lastname: Rungsinaporn
authors.forename: Kanda
authors.initials: K
authors.lastname: Chaipinyo
authors.forename: Kosum
authors.initials: K
authors.lastname: Chansiri
mesh_terms: D000328:Adult; D016026:Bone Marrow Transplantation / Q000379:methods*; D002357:Cartilage Diseases / Q000601:surgery*; D002358:Cartilage, Articular / Q000601:surgery; D020219:Chondrogenesis; D005500:Follow-Up Studies; D006801:Humans; D007719:Knee Joint / Q000601:surgery; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D014182:Transplantation, Autologous; D016896:Treatment Outcome