title: Isolation and characterization of microsatellite loci in an endangered tree species, Afzelia xylocarpa (Kurz) Craib (Caesalpinioideae).
abstract: Afzelia xylocarpa is listed as an endangered species on the International Union for the Conservation of Nature and Natural Resources World list of Threatened Trees, due to overexploitation for its valuable timber and habitat loss. We isolated eight polymorphic microsatellite loci from A. xylocarpa using dual suppression polymerase chain reaction technique. These loci provide microsatellite markers with high polymorphism, as the number of alleles ranged from two to seven, and the estimate of gene diversity was between 0.285 and 0.795. The markers are now available for more detailed investigation of population genetic structure and gene flow among A. xylocarpa populations.
authors.affiliation: Forest Restoration Research Unit, Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand Tree Genetics Laboratory, Department of Forest Genetics, Forestry and Forest Products Research Institute, Tsukuba, Ibaraki 305-8687, Japan Ecological Genetics Laboratory, Department of Forest Genetics, Forestry and Forest Products Research Institute, Tsukuba, Ibaraki 305-8687, Japan.
authors.forename: Greuk
authors.initials: G
authors.lastname: Pakkad
authors.forename: Saneyoshi
authors.initials: S
authors.lastname: Ueno
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yoshimaru