title: Isolation and characterization of 19 polymorphic microsatellite DNA markers in the Japanese brown frog (Rana japonica).
abstract: We report the isolation and characterization of 19 polymorphic microsatellite DNA markers in the Japanese brown frog (Rana japonica). These markers were tested in 24 individuals each collected from three distinct populations in Ichikai-machi, Tochigi Prefecture. The number of observed alleles per locus ranged from 3 to 24 across all populations, and the values of observed and expected heterozygosities ranged from 0.130 to 1 and from 0.125 to 0.941, respectively. These microsatellite loci will be useful for investigating the intraspecific genetic variation and population structure of this species.
authors.affiliation: National Institute for Rural Engineering, Kannondai, Tsukuba, Ibaraki 305-8609, Japan.
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Koizumi
authors.forename: Keiji
authors.initials: K
authors.lastname: Watabe
authors.forename: Atsushi
authors.initials: A
authors.lastname: Mori
authors.forename: Takeshi
authors.initials: T
authors.lastname: Takemura