title: Induction of immune responses in ducks with a DNA vaccine encoding duck plague virus glycoprotein C.
abstract: BACKGROUND
  A DNA vaccine expressing glycoprotein C (gC) of duck plague virus (DPV) was evaluated for inducing immunity in ducks. The plasmid encoding gC of DPV was administered via intramuscular (IM) injection and gene gun bombardment.
  
  
  RESULTS
  After immunization by both routes virus-specific serum antibody and T-cell responses developed. Vaccination of ducks by IM injection induced a stronger humoral, but weaker cell-mediated immune response. In contrast, a better cell-mediated immune response was achieved by using a gene gun to deliver DNA-coated gold beads to the epidermis with as little as 6 μg of DNA.
  
  
  CONCLUSIONS
  This demonstrated that both routes of DNA inoculation can be used for eliciting virus-specific immune responses. Although DNA vaccine containing DPV gC is effective in both intramuscular injection and gene gun bombardment, the latter could induce significantly higher cell-mediated responses against DPV.
authors.affiliation: Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu city, Sichuan, 611130, China.
authors.forename: Bei
authors.initials: B
authors.lastname: Lian
authors.forename: Anchun
authors.initials: A
authors.lastname: Cheng
authors.forename: Mingshu
authors.initials: M
authors.lastname: Wang
authors.forename: Dekang
authors.initials: D
authors.lastname: Zhu
authors.forename: Qihui
authors.initials: Q
authors.lastname: Luo
authors.forename: Renyong
authors.initials: R
authors.lastname: Jia
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
authors.forename: Xinfeng
authors.initials: X
authors.lastname: Han
authors.forename: Xiaoyue
authors.initials: X
authors.lastname: Chen
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology; D019470:Biolistics; D004372:Ducks; D007273:Injections, Intramuscular; D025922:Mardivirus / Q000235:genetics* / Q000276:immunology*; D010957:Plasmids; D011201:Poultry Diseases / Q000276:immunology / Q000517:prevention & control*; D013601:T-Lymphocytes / Q000276:immunology; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D019444:Vaccines, DNA; D014759:Viral Envelope Proteins; D014765:Viral Vaccines