title: Panning and immune electron-microscopic study of mucinous ovarian-cancer antigen-specific cells derived from malignantly transformed bloom-syndrome B-lymphoblastoid cells.
abstract: We separated characteristic mucinous ovarian cancer (OVC) antigen cells from malignantly transformed Bloom syndrome (BS) cell line (BS-SHI-4M) with the panning procedure using OVC patients sera. We undertook an immune electron-microscopic and scanning electron-microscopic study to acquire information regarding the antigenic determinant of the membrane using pre-embedding method, as well as immunofluorescence (IF) study. The distribution of Protein A colloidal gold (PAG) grains on the cell membrane of mucinous OVC antigen cells paralleled that of fluorescein-conjugated anti-human IgG observed in the IF study. The three patterns of PAG labeling of uniform labeling, uniform partial labeling, and partial labeling of one side of the cell paralleled the three patterns of IF labeling observed under IF. These findings strongly suggest the immunological reaction of BS-SHI-4M OVC-MU antigen cells with the antibody of mucinous OVC patient serum. Western blot analysis demonstrated that the antigen which characterizes mucinous OVC has a band at 84000 MW.
authors.affiliation: KOCHI MED SCH,DEPT OBSTET & GYNECOL,MED RES LAB,NANKOKU 783,KOCHI,JAPAN. NARA MED UNIV,DEPT ANAT,KASHIHARA,NARA 634,JAPAN.
authors.forename: Y
authors.initials: Y
authors.lastname: Shiraishi
authors.forename: M
authors.initials: M
authors.lastname: Ozawa
authors.forename: K
authors.initials: K
authors.lastname: Yamamoto
authors.forename: K
authors.initials: K
authors.lastname: Yagyu
authors.forename: K
authors.initials: K
authors.lastname: Nakayama
authors.forename: Y
authors.initials: Y
authors.lastname: Sagara
authors.forename: A
authors.initials: A
authors.lastname: Takakusu