title: Characterization of hyaluronic acid interaction with calcium oxalate crystals: implication of crystals faces, pH and citrate.
abstract: Interaction between hyaluronic acid (HA) present at the surface of tubular epithelial cells and calcium oxalate monohydrate (COM) crystals is thought to play an important role in kidney stone formation. AFM-based force spectroscopy, where HA is covalently attached to AFM-probes, was used to quantify the interaction between HA and the surfaces of COM crystals. The work of adhesion of the HA-probe as well as the rupture force of single HA molecules were quantified in order to understand the molecular regulation of HA binding to COM crystals. Our results reveal that HA adsorbs to the crystal surface in physiological conditions. We also observed increased adhesion when the pH is lowered to a value that increases the risk of kidney stone formation. HA adhesion to the COM crystal surface can be suppressed by citrate, a physiological inhibitor of stone retention currently used in the treatment and prevention of kidney stone formation. Interestingly, we also observed preferential binding of HA onto the [100] face versus the [010] face, suggesting a major contribution of the [100] faces in the crystal retention process at the surface of tubular epithelial cells and the promotion of stone formation. Our results clearly establish a direct role for the glycosaminoglycan HA present at the surface of kidney tubular epithelium in the process of COM crystal retention.
authors.affiliation: Département de Pharmacologie, Université de Sherbrooke, Sherbrooke, QC, J1H-5N4, Canada.
authors.forename: Charles-Antoine
authors.initials: CA
authors.lastname: Lamontagne
authors.forename: Gérard E
authors.initials: GE
authors.lastname: Plante
authors.forename: Michel
authors.initials: M
authors.lastname: Grandbois
mesh_terms: D002129:Calcium Oxalate / Q000737:chemistry*; D019343:Citric Acid / Q000737:chemistry*; D006820:Hyaluronic Acid / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D018625:Microscopy, Atomic Force
chemical_list: D002129:Calcium Oxalate; D019343:Citric Acid; D006820:Hyaluronic Acid