title: Flow cytometric immunophenotypic assessment of T-cell clonality by Vβ repertoire analysis: detection of T-cell clonality at diagnosis and monitoring of minimal residual disease following therapy.
abstract: Flow cytometric T-cell receptor (TCR)-V(β) repertoire analysis (TCR-V(β)-R) is a sensitive method for detection of T-cell clonality; however, no uniform approach exists to define clonality in neoplastic T cells. TCR-V(β)-R was evaluated in patients with a diagnosis of T-cell neoplasia in initial diagnostic specimens from 41 patients and for minimal residual disease (MRD) monitoring in 61 sequential samples from 14 patients with mature T-cell neoplasia. Gating strategies and criteria for detection of T-cell clonality were determined. In all 41 initial specimens, T-cell clonality was demonstrated via TCR-V(β)-R. The frequency of V(β) usage was consistent with random neoplastic transformation of TCR-V(β) subsets. MRD was successfully detected in follow-up samples from all 14 patients evaluated, Furthermore, MRD after therapy was quantitated in 48 peripheral blood specimens. TCR-V(β)-R analysis is a sensitive method for detection of T-cell clonality and is useful for diagnosis and MRD detection in multiple specimen types.
authors.affiliation: Flow Cytometry Unit, Laboratory of Pathology, National Cancer Institute/NIH, Bethesda, MD 20892, USA.
authors.forename: Prashant
authors.initials: P
authors.lastname: Tembhare
authors.forename: Constance M
authors.initials: CM
authors.lastname: Yuan
authors.forename: Liqiang
authors.initials: L
authors.lastname: Xi
authors.forename: John C
authors.initials: JC
authors.lastname: Morris
authors.forename: David
authors.initials: D
authors.lastname: Liewehr
authors.forename: David
authors.initials: D
authors.lastname: Venzon
authors.forename: John E
authors.initials: JE
authors.lastname: Janik
authors.forename: Mark
authors.initials: M
authors.lastname: Raffeld
authors.forename: Maryalice
authors.initials: M
authors.lastname: Stetler-Stevenson
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002471:Cell Transformation, Neoplastic / Q000473:pathology; D002999:Clone Cells / Q000378:metabolism / Q000473:pathology; D005260:Female; D005434:Flow Cytometry; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; D015490:HTLV-I Infections / Q000150:complications; D006801:Humans; D016130:Immunophenotyping / Q000379:methods*; D015458:Leukemia, T-Cell / Q000378:metabolism / Q000473:pathology* / Q000628:therapy / Q000821:virology; D016399:Lymphoma, T-Cell / Q000378:metabolism / Q000473:pathology* / Q000628:therapy / Q000821:virology; D008297:Male; D008875:Middle Aged; D018365:Neoplasm, Residual / Q000097:blood / Q000175:diagnosis* / Q000235:genetics; D016133:Polymerase Chain Reaction; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000097:blood*; D013601:T-Lymphocytes / Q000378:metabolism* / Q000473:pathology*; D055815:Young Adult
chemical_list: D016693:Receptors, Antigen, T-Cell, alpha-beta