title: [Effects of injectable chitosan thermosensitive hydrogel on dog bone marrow stromal cells in vitro].
abstract: OBJECTIVE
  To investigate the biological effects of injectable chitosan(CS) thermosensitive hydrogel on dog bone marrow stromal cells(BMSCs) in vitro.
  
  
  METHODS
  Dog BMSCs were obtained from dog bone marrow aspiration by density gradient centrifugation method, cultured in DMEM medium with 10% fetal bovine serum (FBS) and identified. Chitosan thermosensitive hydrogel was prepared and the BMSCs cultured in a medium containing hydrogel were observed under light microscope. The extract liquid from hydrogel was made and its effects on cell proliferation and differentiation were evaluated. Statistical significance was assessed with SPSS 13.0 software package for one -way ANOVA.
  
  
  RESULTS
  Dog BMSCs survived in the extract liquid and there was no significant difference. The proliferation of BMSCs in mineralization condition medium was lower than normal medium after 1,4,7 days culture and was similar to that in normal medium after 10 days. The osteogenic extract liquid improved the secretion of alkaline phosphatase (ALP) and osteocalcin (OC).
  
  
  CONCLUSIONS
  The CS thermosensitive hydrogel possesses good biocompatibility and can be used for in vitro and in vivo experimental studies of CS hydrogel loaded with BMSCs.
authors.affiliation: Institute of Stomatology, General Hospital of Chinese PLA, Beijing 100853,China. hanxue282004@sina.com
authors.forename: Xue
authors.initials: X
authors.lastname: Han
authors.forename: Hong-chen
authors.initials: HC
authors.lastname: Liu
authors.forename: Dong-sheng
authors.initials: DS
authors.lastname: Wang
authors.forename: Fang
authors.initials: F
authors.lastname: Su
authors.forename: Ling-Ling
authors.initials: LL
authors.lastname: E
authors.forename: Xia
authors.initials: X
authors.lastname: Wu
mesh_terms: D000469:Alkaline Phosphatase; D000818:Animals; D001854:Bone Marrow Cells; D002417:Cattle; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D048271:Chitosan; D004285:Dogs; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D066298:In Vitro Techniques; D059630:Mesenchymal Stem Cells
chemical_list: D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D048271:Chitosan; D000469:Alkaline Phosphatase