title: Lipogenic gene expression in abdominal adipose and liver tissues of diet-induced overweight cats.
abstract: The effect of overweight status on the expression of SREBP-1c and downstream lipogenic genes, such as ATP citrate lyase (ACL) and fatty acid synthase (FAS), in abdominal adipose and liver tissues was determined in cats using a diet-induced weight gain model. ACL and SREBP-1c mRNA expression was significantly reduced (∼65% and 20%, respectively) in liver tissue, whereas FAS and SREBP-1c expression was significantly increased (∼80% and 45%, respectively) in abdominal omental adipose tissue of overweight animals as compared to healthy animals. Additionally, ACL, FAS, and SREBP-1c expression was significantly reduced by ∼50%, 75%, and 70%, respectively, in abdominal subcutaneous adipose tissue of overweight animals. Omental adipose tissue appeared to foster, whereas subcutaneous adipose and liver tissues appeared to defer lipid storage based on differences in SREBP-1c mRNA expression. Overall, reduced lipogenic gene mRNA expression patterns support the hypothesis that SREBP-1c expression is reduced in overweight and possibly obese cats, reflecting down-regulation of the lipogenic pathway to prevent further fat accumulation and weight gain.
authors.affiliation: Department of Veterinary Science, School of Veterinary Medicine, 1-7-1 Kyonancho, Musashino, Tokyo, Japan. Electronic address: peter.lee@mail.mcgill.ca.
authors.forename: Peter
authors.initials: P
authors.lastname: Lee
authors.affiliation: Department of Veterinary Nursing & Technology, School of Veterinary Medicine, 1-7-1 Kyonancho, Musashino, Tokyo, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Mori
authors.affiliation: Department of Veterinary Science, School of Veterinary Medicine, 1-7-1 Kyonancho, Musashino, Tokyo, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Takemitsu
authors.affiliation: Department of Veterinary Science, School of Veterinary Medicine, 1-7-1 Kyonancho, Musashino, Tokyo, Japan.
authors.forename: Ichiro
authors.initials: I
authors.lastname: Yamamoto
authors.affiliation: Department of Veterinary Science, School of Veterinary Medicine, 1-7-1 Kyonancho, Musashino, Tokyo, Japan.
authors.forename: Toshiro
authors.initials: T
authors.lastname: Arai
mesh_terms: D001275:ATP Citrate (pro-S)-Lyase / Q000235:genetics / Q000378:metabolism*; D050153:Abdominal Fat / Q000378:metabolism*; D000818:Animals; D002371:Cat Diseases / Q000378:metabolism*; D002415:Cats; D015536:Down-Regulation; D064429:Fatty Acid Synthases / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D008099:Liver / Q000378:metabolism*; D008297:Male; D050177:Overweight / Q000378:metabolism / Q000662:veterinary*; D010865:Pilot Projects; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D051780:Sterol Regulatory Element Binding Protein 1 / Q000235:genetics / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D051780:Sterol Regulatory Element Binding Protein 1; D064429:Fatty Acid Synthases; D001275:ATP Citrate (pro-S)-Lyase