title: New polymorphic microsatellite markers for the limpet Patella rustica and cross-priming testing in four Patella species.
abstract: Eighteen microsatellite markers have been characterized from the Patella rustica genome. An average of 10 alleles per locus and an expected heterozygosity ranging from 0.054 to 0.937 were observed in a sample of 32 wild individuals from Viana do Castelo, Portugal. Distinct cross-priming amplification rates were recovered on four additional Patella species. Three monomorphic P. rustica loci were polymorphic in other Patella species. The microsatellites developed herein could be a useful intraspecific genetic tool to undertake fine population studies in the genus Patella.
authors.affiliation: CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Campus Agrário de Vairão, 4485-661 Vairão, Portugal.
authors.forename: M
authors.initials: M
authors.lastname: Pérez
authors.forename: A
authors.initials: A
authors.lastname: Llavona
authors.forename: P
authors.initials: P
authors.lastname: Presa
authors.forename: P
authors.initials: P
authors.lastname: Ribeiro
authors.forename: S J
authors.initials: SJ
authors.lastname: Hawkins
authors.forename: A M
authors.initials: AM
authors.lastname: Santos
authors.forename: P
authors.initials: P
authors.lastname: Alexandrino