title: Gene expression analysis of leprosy by using a multiplex branched DNA assay.
abstract: Leprosy is caused by Mycobacterium leprae, and the global registered prevalence of leprosy at the beginning of 2009 stood at 213,036 cases. It has long been thought that leprosy has a strong genetic risk. Recently, we have identified significant associations (P < 1.00 × 10(-10)) between SNPs in the genes CCDC122, C13orf31, NOD2, TNFSF15, HLA-DR and RIPK2 and a trend towards an association (P = 5.10 × 10(-5)) with a SNP in LRRK2. Here, we investigated the expression of these seven genes in formalin-fixed, paraffin-embedded skin tissues of leprosy and matched normal tissues using branched DNA technology. This technology allows for direct measurement of targeted mRNA within cellular lysate using a 96-well plate format in a time frame compared to a reporter gene assay. The clear upregulation of all seven genes was found in leprosy tissues compared to normal tissues, which further supports our genome-wide association study results.
authors.affiliation: Shandong Provincial Institute of Dermatology and Venereology, Provincial Academy of Medical Science, Jinan, Shandong, China.
authors.forename: Yonghu
authors.initials: Y
authors.lastname: Sun
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.forename: Gongqi
authors.initials: G
authors.lastname: Yu
authors.forename: Xuechao
authors.initials: X
authors.lastname: Chen
authors.forename: Huaxu
authors.initials: H
authors.lastname: Liu
authors.forename: Hongqing
authors.initials: H
authors.lastname: Tian
authors.forename: Guizhi
authors.initials: G
authors.lastname: Zhou
authors.forename: Furen
authors.initials: F
authors.lastname: Zhang
mesh_terms: D021121:Branched DNA Signal Amplification Assay; D016022:Case-Control Studies; D020022:Genetic Predisposition to Disease; D055106:Genome-Wide Association Study; D006684:HLA-DR Antigens / Q000235:genetics; D006801:Humans; D007918:Leprosy / Q000235:genetics*; D000071158:Leucine-Rich Repeat Serine-Threonine Protein Kinase-2; D053473:Nod2 Signaling Adaptor Protein / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D017346:Protein Serine-Threonine Kinases / Q000235:genetics; D053475:Receptor-Interacting Protein Serine-Threonine Kinase 2 / Q000235:genetics; D012307:Risk Factors; D053380:Tumor Necrosis Factor Ligand Superfamily Member 15 / Q000235:genetics
chemical_list: D006684:HLA-DR Antigens; C429018:NOD2 protein, human; D053473:Nod2 Signaling Adaptor Protein; C117026:TNFSF15 protein, human; D053380:Tumor Necrosis Factor Ligand Superfamily Member 15; C495280:LRRK2 protein, human; D000071158:Leucine-Rich Repeat Serine-Threonine Protein Kinase-2; D017346:Protein Serine-Threonine Kinases; C506253:RIPK2 protein, human; D053475:Receptor-Interacting Protein Serine-Threonine Kinase 2