title: MicroRNA-126 inhibits ischemia-induced retinal neovascularization via regulating angiogenic growth factors.
abstract: To investigate the potential transcriptional regulation and signal pathway of a single microRNA in ischemia-induced retinal neovascularization (NV), we used oxygen-induced retinopathy (OIR) in establishing retinal NV model, and quantitative real-time reverse transcriptase PCR analyzing a microRNA (miR-126) alteration. The mice were treated with plasmid pCMV-MIR-126/liposome mixture intravitreal injection, using pCMV-MIR/liposome mixture as control. The expression levels of VEGF, IGF-2 and HIF-1α, and the level changes of total and phosphorylated p38, ERK in retina from OIR mice were determined by western blot analysis. The effects of miR-126 on retinal NV in OIR mice were identified with fluoresecin angiography and H & E staining. No effect of miR-126 intravitreal injection on retinal vessels was performed with CD31 stained retinal sections. Our results showed that miR-126 was significantly decreased in retina from OIR mice. We confirmed that restoration of miR-126 in retina overcame the high levels of VEGF, IGF-2 and HIF-1α through downregulating p38 and ERK signaling molecules in OIR model, and that miR-126 intravitreal injection reduced retinal NV in OIR model. These results suggest that miR-126 might play a potential transcriptional role in the pathogenesis in diabetic retinopathy.
authors.affiliation: Department of Medicine/Cell Biology, Soochow University, Suzhou 215123, China. yy_bai@yahoo.com
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Bai
authors.forename: Xia
authors.initials: X
authors.lastname: Bai
authors.forename: Zhaoyue
authors.initials: Z
authors.lastname: Wang
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Zhang
authors.forename: Changgeng
authors.initials: C
authors.lastname: Ruan
authors.forename: Jingcheng
authors.initials: J
authors.lastname: Miao
mesh_terms: D042501:Angiogenic Proteins / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D015415:Biomarkers / Q000378:metabolism; D049109:Cell Proliferation; D004195:Disease Models, Animal; D015536:Down-Regulation; D005451:Fluorescein Angiography; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D018496:Hyperoxia / Q000378:metabolism / Q000473:pathology; D007511:Ischemia / Q000188:drug therapy*; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000494:pharmacology*; D010100:Oxygen / Q000633:toxicity; D015861:Retinal Neovascularization / Q000188:drug therapy* / Q000378:metabolism; D012171:Retinal Vessels / Q000187:drug effects*
chemical_list: D042501:Angiogenic Proteins; D015415:Biomarkers; C545201:MIRN126 microRNA, mouse; D035683:MicroRNAs; D010100:Oxygen