title: A confirmatory method for aflatoxin M₁ determination in milk based on immunoaffinity cleanup and high-performance liquid chromatography with fluorometric detection.
abstract: A sensitive and reliable analytical method based on immunoaffinity chromatography cleanup followed by HPLC separation and fluorimetric detection is described for the quantitative determination of aflatoxin M(1) in milk. The chromatographic separation is accomplished by using a C18 column and a gradient elution with methanol, acetonitrile, and water. No extraction solvent process is required and a minimal milk sample cleanup is performed by a direct loading of the immunoaffinity columns and elution with methanol. The method has been successfully validated according to Decision EC No 657/2002 by using the conventional validation approach. The results of the validation process demonstrate the agreement of the method with the provisions of Regulation EC No 401/2006.
authors.affiliation: Dipartimento di Scienze Agro-Ambientali, Chimica e Difesa Vegetale and BIOAGROMED, Università degli Studi di Foggia, Foggia, Italy.
authors.forename: Marilena
authors.initials: M
authors.lastname: Muscarella
authors.forename: Sonia Lo
authors.initials: SL
authors.lastname: Magro
authors.forename: Carmen
authors.initials: C
authors.lastname: Palermo
authors.forename: Diego
authors.initials: D
authors.lastname: Centonze
mesh_terms: D016607:Aflatoxin M1 / Q000032:analysis*; D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015200:Fluoroimmunoassay / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D008892:Milk / Q000737:chemistry* / Q000382:microbiology
chemical_list: D016607:Aflatoxin M1