title: Lewis X oligosaccharides-heparanase complex targeting to DCs enhance antitumor response in mice.
abstract: Heparanase has been proved as an promising tumor antigen for the therapeutical target. However, the antigen alone cannot fully elicit the immune response in vivo. In this study, Lewis X oligosaccharides-heparanase complex was prepared, which can target to the dendritic cells (DCs) via dendritic cell-specific intercellular-adhesion-molecule-grabbing non-integrin (DC-SIGN). In addition, the DCs were loaded with the complex, and then were utilized to immunize mice to detect the immune response. Our data demonstrated that the modified DCs could enhance the specific IFN-γ production and cytotoxic T cell response. Furthermore, the modified DCs could also significantly suppress the established tumor growth and prolong the life span of tumor-bearing mice. Therefore, the Lewis X oligosaccharides-heparanase complex might be regarded as an ideal vaccine, and represent a novel way for the therapeutical strategy of tumor.
authors.affiliation: Department of Neurosurgery, Fuzhou General Hospital of Nanjing Command, Fuzhou, Fujian 350025, China.
authors.forename: Hongjie
authors.initials: H
authors.lastname: Chen
authors.forename: Bangqing
authors.initials: B
authors.lastname: Yuan
authors.forename: Zhaocong
authors.initials: Z
authors.lastname: Zheng
authors.forename: Zheng
authors.initials: Z
authors.lastname: Liu
authors.forename: Shousen
authors.initials: S
authors.lastname: Wang
mesh_terms: D000178:Acrylamides / Q000737:chemistry; D000180:Acrylic Resins; D000818:Animals; D001710:Biotin / Q000031:analogs & derivatives / Q000737:chemistry; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D019496:Cancer Vaccines / Q000138:chemical synthesis / Q000276:immunology / Q000627:therapeutic use*; D045744:Cell Line, Tumor; D003601:Cytotoxicity Tests, Immunologic; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism / Q000637:transplantation; D005966:Glucuronidase / Q000737:chemistry / Q000276:immunology*; D007371:Interferon-gamma / Q000378:metabolism; D007983:Lewis Blood Group Antigens; D051379:Mice; D008815:Mice, Inbred Strains; D009369:Neoplasms / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D009844:Oligosaccharides / Q000737:chemistry* / Q000378:metabolism; D019809:Streptavidin / Q000737:chemistry; D016019:Survival Analysis; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014614:Vaccines, Synthetic / Q000737:chemistry / Q000276:immunology; D000099175:Heparanase; D000099166:Polyacrylamides
chemical_list: D000178:Acrylamides; D000180:Acrylic Resins; D001710:Biotin; D019496:Cancer Vaccines; D005966:Glucuronidase; D007371:Interferon-gamma; D007983:Lewis Blood Group Antigens; D009844:Oligosaccharides; D019809:Streptavidin; D014614:Vaccines, Synthetic; D000099175:Heparanase; C494219:Lewis a oligosaccharide; C501519:biotinylated sulfo-Lewis A derivatized polyacrylamide; D000099166:Polyacrylamides