title: Identification of salt-tolerant Sinorhizobium sp. strain BL3 membrane proteins based on proteomics.
abstract: Sinorhizobium sp. BL3 is a salt-tolerant strain that can fix atmospheric nitrogen in symbiosis with leguminous host plants under salt-stress conditions. Since cell membranes are the first barrier to environmental change, it is interesting to explore the membrane proteins within this protective barrier under salt stress. The protein contents of membrane-enriched fractions obtained from BL3 were analyzed by nanoflow liquid chromatography interfaced with electrospray ionization tandem mass spectrometry. A total of 105 membrane proteins were identified. These proteins could be classified into 17 functional categories, the two biggest of which were energy production and conversion, and proteins not in clusters of orthologous groups (COGs). In addition, a comparative analysis of membrane proteins between salt-stressed and non-stressed BL3 cells was conducted using a membrane enrichment method and off-line SCX fractionation coupled to nanoLC-MS/MS. These techniques would be useful for further comparative analysis of membrane proteins that function in the response to environmental stress.
authors.affiliation: Synchrotron Light Research Institute (Public Organization), Suranaree, Muang, Nakhon Ratchasima, 30000, Thailand.
authors.forename: Waraporn
authors.initials: W
authors.lastname: Tanthanuch
authors.forename: Panlada
authors.initials: P
authors.lastname: Tittabutr
authors.forename: Shabaz
authors.initials: S
authors.lastname: Mohammed
authors.forename: Rune
authors.initials: R
authors.lastname: Matthiesen
authors.forename: Montarop
authors.initials: M
authors.lastname: Yamabhai
authors.forename: Monchai
authors.initials: M
authors.lastname: Manassila
authors.forename: Ole Noerregaard
authors.initials: ON
authors.lastname: Jensen
authors.forename: Nantakorn
authors.initials: N
authors.lastname: Boonkerd
authors.forename: Neung
authors.initials: N
authors.lastname: Teaumroong
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D040901:Proteomics; D020579:Sinorhizobium / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012965:Sodium Chloride / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins; D012965:Sodium Chloride