title: Biodegradable CaMgZn bulk metallic glass for potential skeletal application.
abstract: A low density and high strength alloy, Ca65Mg15Zn20 bulk metallic glass (CaMgZn BMG), was evaluated by both in vitro tests on ion release and cytotoxicity and in vivo implantation, aimed at exploring the feasibility of this new biodegradable metallic material for potential skeletal applications. MTT assay results showed that the experimental CaMgZn BMG extracts had no detectable cytotoxic effects on L929, VSMC and ECV304 cells over a wide range of concentrations (0-50%), whereas for MG63 cells concentrations in the range ~5-20% promoted cell viability. Meanwhile, alkaline phosphatase (ALP) activity results showed that CaMgZn BMG extracts increased alkaline phosphatase (ALP) production by MG63 cells. However, Annexin V-fluorescein isothiocyanate and propidium iodide staining indicated that higher concentrations (50%) might induce cell apoptosis. The fluorescence observation of F-actin and nuclei in MG63 cells showed that cells incubated with lower concentrations (0-50%) displayed no significant change in morphology compared with a negative control. Tumor necrosis factor-α expression by Raw264.7 cells in the presence of CaMgZn BMG extract was significantly lower than that of the positive and negative controls. Animal tests proved that there was no obvious inflammation reaction at the implantation site and CaMgZn BMG implants did not result in animal death. The cortical thickness around the CaMgZn BMG implant increased gradually from 1 to 4 weeks, as measured by in vivo micro-computer tomography.
authors.affiliation: State Key Laboratory for Turbulence and Complex Systems and Department of Advanced Materials and Nanotechnology, College of Engineering, Peking University, Beijing 100871, People's Republic of China.
authors.forename: Y B
authors.initials: YB
authors.lastname: Wang
authors.forename: X H
authors.initials: XH
authors.lastname: Xie
authors.forename: H F
authors.initials: HF
authors.lastname: Li
authors.forename: X L
authors.initials: XL
authors.lastname: Wang
authors.forename: M Z
authors.initials: MZ
authors.lastname: Zhao
authors.forename: E W
authors.initials: EW
authors.lastname: Zhang
authors.forename: Y J
authors.initials: YJ
authors.lastname: Bai
authors.forename: Y F
authors.initials: YF
authors.lastname: Zheng
authors.forename: L
authors.initials: L
authors.lastname: Qin
mesh_terms: D000199:Actins / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001672:Biocompatible Materials / Q000494:pharmacology*; D001673:Biodegradation, Environmental / Q000187:drug effects; D015153:Blotting, Western; D001842:Bone and Bones / Q000187:drug effects* / Q000502:physiology*; D002118:Calcium / Q000494:pharmacology; D002460:Cell Line; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D003343:Corrosion; D005456:Fluorescent Dyes / Q000378:metabolism; D005898:Glass / Q000737:chemistry*; D006801:Humans; D020007:Implants, Experimental; D008274:Magnesium / Q000494:pharmacology; D008670:Metals / Q000494:pharmacology*; D051379:Mice; D008855:Microscopy, Electron, Scanning; D012996:Solutions; D023822:Tissue Engineering / Q000379:methods*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D055114:X-Ray Microtomography; D015032:Zinc / Q000494:pharmacology
chemical_list: D000199:Actins; D001672:Biocompatible Materials; D005456:Fluorescent Dyes; D008670:Metals; D012996:Solutions; D014409:Tumor Necrosis Factor-alpha; D000469:Alkaline Phosphatase; D008274:Magnesium; D015032:Zinc; D002118:Calcium