title: The expression of damage-regulated autophagy modulator 2 (DRAM2) contributes to autophagy induction.
abstract: Autophagy is a membrane trafficking process involved in intracellular degradation and recycling in eukaryotic cells. DRAM2 (damage-regulated autophagy modulator 2) is a homologue of DRAM that regulates p53-mediated cell death. As its name implies, DRAM expression induces autophagy in a p53-dependent manner; however, the role of DRAM2 in autophagy is not clear. In this study, we report that DRAM2 expression contributes to autophagy induction. Overexpression of DRAM2 induces cytoplasmic GFP-LC3 punctuates, and increases the level of endogenous LC3-II. Moreover, the silencing of endogenous DRAM2 interferes with starvation-induced autophagy. Thus, we propose that DRAM2 as well as DRAM are involved in autophagy.
authors.affiliation: Division of Biological Science and Technology, Yonsei University, Wonju, 220-710, Republic of Korea.
authors.forename: Jung-Ho
authors.initials: JH
authors.lastname: Yoon
authors.forename: Song
authors.initials: S
authors.lastname: Her
authors.forename: Moonhee
authors.initials: M
authors.lastname: Kim
authors.forename: Ik-Soon
authors.initials: IS
authors.lastname: Jang
authors.forename: Junsoo
authors.initials: J
authors.lastname: Park
mesh_terms: D001343:Autophagy / Q000235:genetics / Q000502:physiology*; D002460:Cell Line; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D015151:Immunoblotting; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D034622:RNA Interference; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: C544299:DRAM2 protein, human; C477012:MAP1LC3A protein, human; D008565:Membrane Proteins; D008869:Microtubule-Associated Proteins; D016159:Tumor Suppressor Protein p53; D049452:Green Fluorescent Proteins