title: Development of polymorphic microsatellite markers for the livebearing fish Poecilia parae.
abstract: We developed 16 novel polymorphic tetranucleotide microsatellite markers for Poecilia parae, a livebearing fish used in evolutionary studies because of its Y-linked colour and size polymorphism. A set of 199 clones was sequenced out of an enriched genomic library, and we achieved an enrichment efficiency of nearly 80%. Primers were designed for 16 pure repeats, and 59 P. parae were screened for polymorphism. Cross-amplification was tested on Poecilia picta and Poecilia reticulata, the guppy. The new microsatellite markers showed an exceptionally high allelic diversity and low stutter formation, proving their suitability for a broad range of applications in these species.
authors.affiliation: Anthropological Institute and Museum, University of Zurich, Winterthurerstrasse 190, 8057 Zürich, Switzerland, Zoological Institute, University of Zurich, Winterthurerstrasse 190, 8057 Zürich, Switzerland.
authors.forename: Alexander
authors.initials: A
authors.lastname: Nater
authors.forename: Michael
authors.initials: M
authors.lastname: Krützen
authors.forename: Anna K
authors.initials: AK
authors.lastname: Lindholm