title: Combined photoacoustic microscopy and optical coherence tomography can measure metabolic rate of oxygen.
abstract: We proposed to measure the metabolic rate of oxygen (MRO(2)) in small animals in vivo using a multimodal imaging system that combines laser-scanning optical-resolution photoacoustic microscopy (LSOR-PAM) and spectral-domain optical coherence tomography (SD-OCT). We first tested the capability of the multimodal system to measure flow rate in a phantom made of two capillary tubes of different diameters. We then demonstrated the capability of measuring MRO(2) by imaging two parallel vessels selected from the ear of a Swiss Webster mouse. The hemoglobin oxygen saturation (sO(2)) and the vessel diameter were measured by the LSOR-PAM and the blood flow velocity was measured by the SD-OCT, from which blood flow rate and MRO(2) were further calculated. The measured blood flow rates in the two vessels agreed with each other.
authors.forename: Tan
authors.initials: T
authors.lastname: Liu
authors.forename: Qing
authors.initials: Q
authors.lastname: Wei
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Shuliang
authors.initials: S
authors.lastname: Jiao
authors.forename: Hao F
authors.initials: HF
authors.lastname: Zhang