title: Eph receptors are involved in the activity-dependent synaptic wiring in the mouse cerebellar cortex.
abstract: Eph receptor tyrosine kinases are involved in many cellular processes. In the developing brain, they act as migratory and cell adhesive cues while in the adult brain they regulate dendritic spine plasticity. Here we show a new role for Eph receptor signalling in the cerebellar cortex. Cerebellar Purkinje cells are innervated by two different excitatory inputs. The climbing fibres contact the proximal dendritic domain of Purkinje cells, where synapse and spine density is low; the parallel fibres contact the distal dendritic domain, where synapse and spine density is high. Interestingly, Purkinje cells have the intrinsic ability to generate a high number of spines over their entire dendritic arborisations, which can be innervated by the parallel fibres. However, the climbing fibre input continuously exerts an activity-dependent repression on parallel fibre synapses, thus confining them to the distal Purkinje cell dendritic domain. Such repression persists after Eph receptor activation, but is overridden by Eph receptor inhibition with EphA4/Fc in neonatal cultured cerebellar slices as well as mature acute cerebellar slices, following in vivo infusion of the EphA4/Fc inhibitor and in EphB receptor-deficient mice. When electrical activity is blocked in vivo by tetrodotoxin leading to a high spine density in Purkinje cell proximal dendrites, stimulation of Eph receptor activation recapitulates the spine repressive effects of climbing fibres. These results suggest that Eph receptor signalling mediates the repression of spine proliferation induced by climbing fibre activity in Purkinje cell proximal dendrites. Such repression is necessary to maintain the correct architecture of the cerebellar cortex.
authors.affiliation: Department of Neuroscience, University of Turin, Turin, Italy.
authors.forename: Roberta
authors.initials: R
authors.lastname: Cesa
authors.forename: Federica
authors.initials: F
authors.lastname: Premoselli
authors.forename: Annamaria
authors.initials: A
authors.lastname: Renna
authors.forename: Iryna M
authors.initials: IM
authors.lastname: Ethell
authors.forename: Elena B
authors.initials: EB
authors.lastname: Pasquale
authors.forename: Piergiorgio
authors.initials: P
authors.lastname: Strata
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002525:Cerebellar Cortex / Q000378:metabolism*; D003712:Dendrites / Q000378:metabolism; D004594:Electrophysiology / Q000379:methods; D005786:Gene Expression Regulation; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008024:Ligands; D051379:Mice; D008822:Mice, Transgenic; D008856:Microscopy, Fluorescence / Q000379:methods; D008954:Models, Biological; D011689:Purkinje Cells / Q000378:metabolism*; D036081:Receptors, Eph Family / Q000378:metabolism / Q000502:physiology*; D013569:Synapses
chemical_list: D008024:Ligands; D049452:Green Fluorescent Proteins; D036081:Receptors, Eph Family