title: CAPS markers using mitochondrial consensus primers for molecular identification of Panax species and Korean ginseng cultivars (Panax ginseng C. A. Meyer).
abstract: Cleaved amplified polymorphic sequence (CAPS) marker system using mitochondrial consensus primers was applied for molecular identification of Korean ginseng cultivars (Panax ginseng). Initially, a total of 34 primers were tested to six Korean ginseng cultivars and two foreign Panax species, P. quinquefolius and P. notoginseng. In the polymerase chain reaction (PCR) amplification results, four primers (mt7, mt11, mt13, and mt18) generated co-dominant polymorphic banding patterns discriminating the Korean ginseng cultivars from P. quinquefolius and P. notoginseng. In the CAPS analysis results, the majority of the cleaved PCR products also yielded additional latent polymorphisms between the Korean ginseng cultivars and two foreign Panax species. Specific latent CAPS polymorphisms for cultivar Gopoong and Chunpoong were detected from internal region amplified with mt9 primer by treating HinfI and Tsp509I endonucleases, respectively. Sequencing analysis revealed that the length of amplified region of Korean ginseng cultivars was 2,179 bp, and those of P. quinquefolius and P. notoginseng were 2,178 and 2,185 bp, respectively. Blast search revealed that the amplified region was a mitochondrial cytochrome oxidase subunit 2 (cox2) gene intron II region. Nineteen single nucleotide polymorphisms (SNP) including each specific SNP for Gopoong and Chunpoong, and three insertion and deletion (InDel) polymorphisms were detected by sequence alignment. The CAPS markers developed in this study, which are specific to Gopoong and Chunpoong, and between the Korean ginseng cultivars and two foreign Panax species, will serve as a practical and reliable tool for their identification, purity maintenance, and selection of candidate lines and cultivars.
authors.affiliation: Division of Forest Genetic Resources, Korea Forest Research Institute, Suwon, 441-847, Korea.
authors.forename: Jei-Wan
authors.initials: JW
authors.lastname: Lee
authors.forename: Kyong-Hwan
authors.initials: KH
authors.lastname: Bang
authors.forename: Young-Chang
authors.initials: YC
authors.lastname: Kim
authors.forename: A-Yeon
authors.initials: AY
authors.lastname: Seo
authors.forename: Ick-Hyun
authors.initials: IH
authors.lastname: Jo
authors.forename: Jeong-Hoon
authors.initials: JH
authors.lastname: Lee
authors.forename: Ok-Tae
authors.initials: OT
authors.lastname: Kim
authors.forename: Dong-Yun
authors.initials: DY
authors.lastname: Hyun
authors.forename: Seon-Woo
authors.initials: SW
authors.lastname: Cha
authors.forename: Joon-Hyeong
authors.initials: JH
authors.lastname: Cho
mesh_terms: D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D004996:Ethidium; D005819:Genetic Markers / Q000235:genetics*; D008969:Molecular Sequence Data; D005894:Panax / Q000235:genetics*; D016133:Polymerase Chain Reaction; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D004272:DNA, Mitochondrial; D005819:Genetic Markers; D004996:Ethidium