title: The apoptotic regulator Nrz controls cytoskeletal dynamics via the regulation of Ca2+ trafficking in the zebrafish blastula.
abstract: Bcl-2 family members are key regulators of apoptosis. Their involvement in other cellular processes has been so far overlooked. We have studied the role of the Bcl-2 homolog Nrz in the developing zebrafish. Nrz was found to be localized to the yolk syncytial layer, a region containing numerous mitochondria and ER membranes. Nrz knockdown resulted in developmental arrest before gastrulation, due to free Ca(2+) increase in the yolk cell, activating myosin light chain kinase, which led to premature contraction of actin-myosin cables in the margin and separation of the blastomeres from the yolk cell. In the yolk syncytial layer, Nrz appears to prevent the release of Ca(2+) from the endoplasmic reticulum by directly interacting with the IP3R1 Ca(2+) channel. Thus, the Bcl-2 family may participate in early development, not only by controlling apoptosis but also by acting on cytoskeletal dynamics and cell movements via Ca(2+) fluxes inside the embryo.
authors.affiliation: CRCL U1052 INSERM, UMS 3443 CNRS, Centre Léon Bérard, 28 rue Laennec, 69008 Lyon, France.
authors.forename: Nikolay
authors.initials: N
authors.lastname: Popgeorgiev
authors.forename: Benjamin
authors.initials: B
authors.lastname: Bonneau
authors.forename: Karine F
authors.initials: KF
authors.lastname: Ferri
authors.forename: Julien
authors.initials: J
authors.lastname: Prudent
authors.forename: Julien
authors.initials: J
authors.lastname: Thibaut
authors.forename: Germain
authors.initials: G
authors.lastname: Gillet
mesh_terms: D000818:Animals; D017209:Apoptosis; D036703:Blastula / Q000166:cytology / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D003599:Cytoskeleton / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000378:metabolism*
chemical_list: D011518:Proto-Oncogene Proteins; D029961:Zebrafish Proteins; C514486:bcl2l10 protein, zebrafish; D002118:Calcium