title: A cell-based fluorescent assay to detect the activity of Shiga toxin and other toxins that inhibit protein synthesis.
abstract: Escherichia coli O157:H7, a major cause of food-borne illness, produces Shiga toxins (Stxs) that block protein synthesis by inactivating the ribosome. In this chapter, we describe a simple cell-based fluorescent assay to detect Stxs and inhibitors of toxin activity. The assay can also be used to detect other plant and bacterial toxins that arrest protein synthesis.
authors.affiliation: Biomolecular Research Annex, Orlando, FL, USA.
authors.forename: Shane
authors.initials: S
authors.lastname: Massey
authors.forename: Beatriz
authors.initials: B
authors.lastname: Quiñones
authors.forename: Ken
authors.initials: K
authors.lastname: Teter
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D019453:Escherichia coli O157 / Q000302:isolation & purification* / Q000472:pathogenicity; D005506:Food Contamination / Q000032:analysis; D059022:Food Safety; D049452:Green Fluorescent Proteins / Q000096:biosynthesis; D014176:Protein Biosynthesis; D022641:Shiga Toxin 2 / Q000032:analysis* / Q000037:antagonists & inhibitors; D014709:Vero Cells
chemical_list: D022641:Shiga Toxin 2; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins