title: PERMANENT GENETIC RESOURCES: Isolation and characterization of microsatellite loci in the Mediterranean shore crab Carcinus aestuarii (Decapoda, Portunidae).
abstract: We characterized nine polymorphic microsatellites in the Mediterranean shore crab Carcinus aestuarii (Decapoda: Portunidae). Microsatellites were isolated from a partial genomic library enriched for multiple motifs. All loci were polymorphic, with number of alleles ranging from two to 16 and a mean observed heterozygosity of 0.75. Seven loci were in Hardy-Weinberg equilibrium, and two showed weak heterozygote deficiency. No linkage disequilibrium was found between loci. In addition, we tested Hardy-Weinberg and linkage equilibrium of three Carcinus maenas loci, already reported to cross-amplify in C. aestuarii. These molecular markers will be potentially useful to investigate genetic structure of this species.
authors.affiliation: Department of Biology, University of Padova, via U. Bassi 58/B, I-35121 Padova, Italy.
authors.forename: Ilaria A M
authors.initials: IA
authors.lastname: Marino
authors.forename: Federica
authors.initials: F
authors.lastname: Barbisan
authors.forename: Micol
authors.initials: M
authors.lastname: Gennari
authors.forename: Paolo Maria
authors.initials: PM
authors.lastname: Bisol
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Zane