title: Wild-type p53 is not involved in reversion of the tumorigenic phenotype of breast-cancer cells after transfer of normal chromosome-17.
abstract: A number of different candidate tumor suppressor genes involved in human breast cancer are presumed to be located on chromosome 17. To verify the relevance of chromosome 17 abnormalities in breast cancer cells, a normal human chromosome 17 was transferred by microcell fusion to R30 tumor cells derived from an infiltrating ductal mammary carcinoma. The tumorigenicity of the microcell hybrids in nude mice was examined. The tumor volume obtained with different clones was reduced by up to 94% of the value corresponding to the parental tumor cells. This effect was accompanied by a reduction of anchorage-independent growth, as well as cell growth rates on plastic plates. These effects were independent of the continued presence of a transferred 17q arm and could not be attributed to the action of the normal p53 gene. The results support the assumption that in addition to p53 a further tumor suppressor gene is located on 17p which is involved in breast cancer.
authors.affiliation: MAX DELBRUCK CTR MOLEK MED,BEREICH TUMOR THERAPIE,D-13122 BERLIN,GERMANY. WITEGA EV,D-13122 BERLIN,GERMANY. HEINE PETTE INST EXPT VIROL & IMMUNOL,TUMOR VIROL ABT,D-20251 HAMBURG,GERMANY. NIEHS,MOLEC CARCINOGENESIS LAB,RES TRIANGLE PK,NC 27709.
authors.forename: M
authors.initials: M
authors.lastname: Theile
authors.forename: S
authors.initials: S
authors.lastname: Hartmann
authors.forename: H
authors.initials: H
authors.lastname: Naundorf
authors.forename: D
authors.initials: D
authors.lastname: Ruess
authors.forename: B
authors.initials: B
authors.lastname: Elbe
authors.forename: H
authors.initials: H
authors.lastname: Krause
authors.forename: W
authors.initials: W
authors.lastname: Deppert
authors.forename: J
authors.initials: J
authors.lastname: Barrett
authors.forename: S
authors.initials: S
authors.lastname: Scherneck