title: The role of stroma cells on the proliferation and C-kit expression of acute myeloblastic-leukemia cells.
abstract: To examine the role of stroma cells on the proliferation and c-kit expression of acute myeloblastic leukemia (AML) cells, blast cells from patients were cultured with growth factors or on human marrow stroma cells. c-KIT protein detected by flow cytometry and the plating efficiencies of the cells cultured with growth factors decreased markedly, but those of the cells cultured on stroma cells were mostly preserved. Conditioned medium from the stroma cells and fibroblasts from fetal Sl/Sl mouse showed, to some extent, the same effects. The growth factors including kit ligand seemed to favor the terminal division more than the self renewal and stroma cells had the opposite tendency. Stoma cells may produce some factors other than kit ligand to maintain c-kit expression and high clonogenicity.
authors.affiliation: ONTARIO CANC INST,TORONTO M4X 1K9,ONTARIO,CANADA. HANSON CTR,LEUKEMIA RES UNIT,ADELAIDE,SA 5000,AUSTRALIA.
authors.forename: S
authors.initials: S
authors.lastname: Tohda
authors.forename: L
authors.initials: L
authors.lastname: Ashman
authors.forename: M
authors.initials: M
authors.lastname: Minden