title: Reaction of rat connective tissue to Mineral Trioxide Aggregate and Diaket.
abstract: BACKGROUND
  The aim of this study was to compare the reaction of rat connective tissue to two root-end filling materials: white Mineral Trioxide Aggregate (WMTA) and Diaket.
  
  
  METHODS
  Each of the materials was placed in dentine tubes and implanted subcutaneously in the dorsal connective tissue of 21 Wistar albino rats. Tissue biopsies were collected 7, 30, and 60 days after the implantation procedure. The specimens were processed and stained with hematoxylin and eosin and examined microscopically. After determining inflammatory cell numbers in sections from each specimen, inflammatory reaction scores were defined as follows: 0; no or few inflammatory cells (no reaction), 1; less than 25 cells (mild reaction), 2; 25 to 125 cells, (moderate reaction), and 3; 125 or more cells (severe reaction). Statistical analysis was performed using the Kruskal-Wallis and Mann-Whitney tests.
  
  
  RESULTS
  There were statistically significant differences in the median inflammatory cell numbers throughout the three test periods, with the most severe degree of inflammation observed at the one-week period. Few cases of necrosis were observed with WMTA. Diaket exhibited the most severe degree of inflammation and necrosis. After 30 days, both materials provoked moderate inflammatory reaction. The eight-week period showed the least severe degree of inflammation in all groups.
  
  
  CONCLUSIONS
  It was concluded that WMTA exhibits a more favourable tissue response compared with Diaket which induced more severe inflammatory reaction than WMTA and the control.
authors.affiliation: Department of Conservative Dentistry, Faculty of Dentistry, Jordan University of Science and Technology, Irbid, Jordan. womari@just.edu.jo
authors.forename: Wael M
authors.initials: WM
authors.lastname: Al-Omari
authors.forename: Maisoun S
authors.initials: MS
authors.lastname: Abu-Zaghlan
authors.forename: Huda M
authors.initials: HM
authors.lastname: Hammad
mesh_terms: D017607:Aluminum Compounds / Q000633:toxicity*; D000818:Animals; D001729:Bismuth / Q000633:toxicity*; D017610:Calcium Compounds / Q000633:toxicity*; D003238:Connective Tissue / Q000187:drug effects*; D003804:Dentin; D004338:Drug Combinations; D006801:Humans; D007249:Inflammation / Q000139:chemically induced; D008297:Male; D010087:Oxides / Q000633:toxicity*; D011145:Polyvinyls / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D012387:Root Canal Filling Materials / Q000633:toxicity*; D017640:Silicates / Q000633:toxicity*; D018709:Statistics, Nonparametric; D013997:Time Factors; D015034:Zinc Oxide / Q000633:toxicity*
chemical_list: D017607:Aluminum Compounds; D017610:Calcium Compounds; D004338:Drug Combinations; D010087:Oxides; D011145:Polyvinyls; D012387:Root Canal Filling Materials; D017640:Silicates; C086631:mineral trioxide aggregate; C014457:bismuth phosphate, copolymers of vinyl acetate, dichlorophen, propionyl-acetophenone, triethanolamine, vinyl chloride, vinylisobutyl ether, zinc oxide drug combination; D015034:Zinc Oxide; D001729:Bismuth