title: [Expression of LOX and MMP-2 in gastric cancer tissue and the effects of LOX and MMP-2 on tumor invasion and metastasis].
abstract: OBJECTIVE
  To compare the expressions of lysyl oxidase (LOX) and matrix metalloproteinases-2 (MMP-2) in gastric cancer and pericancerous tissues, in gastric cancers with and without lymph node metastasis, and to analyze the effects of LOX and MMP-2 on tumor invasion and metastasis.
  
  
  METHODS
  Gastric cancer and pericancerous tissues were collected from 46 patients who underwent surgery. Levels of LOX and MMP-2 mRNA were detected by RT-PCR. Protein abundance of LOX and MMP-2 was examined using Western blot.
  
  
  RESULTS
  Expressions of LOX and MMP-2 mRNA, and protein in 46 gastric cancers were significantly higher than that in 46 pericancerous tissues. In gastric cancer with lymph node metastasis, the levels of LOX and MMP-2 mRNA and protein were higher than those in gastric cancers without lymph node metastasis (P < 0.05). In the groups of gastric cancer with lymph node metastasis, expression of LOX was positively correlated with MMP-2 protein expression (P < 0.01).
  
  
  CONCLUSIONS
  Expressions of LOX and MMP-2 in gastric cancer tissues are significantly higher than that in pericancerous tissues. The expressions of LOX and MMP-2 in gastric cancer with lymph node metastasis are higher than that in gastric cancer without lymph node metastasis. Expressions of LOX and MMP-2 are positively correlated. The results suggest that LOX and MMP-2 may promote the growth and metastasis of gastric cancer.
authors.affiliation: The Second Affiliated Hospital of Ningxia Medical University, Yingchuan 750001, China.
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Ma
authors.forename: Yi-Gong
authors.initials: YG
authors.lastname: Li
authors.forename: Ling
authors.initials: L
authors.lastname: Huang
authors.forename: Mei
authors.initials: M
authors.lastname: Han
authors.forename: Bai-Jing
authors.initials: BJ
authors.lastname: Ma
authors.forename: Bo-Jian
authors.initials: BJ
authors.lastname: Sun
authors.forename: Jia-Jing
authors.initials: JJ
authors.lastname: Lin
authors.forename: Ting-Ge
authors.initials: TG
authors.lastname: Song
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D014408:Biomarkers, Tumor / Q000378:metabolism; D005260:Female; D005743:Gastrectomy; D005753:Gastric Mucosa / Q000378:metabolism; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D008249:Protein-Lysine 6-Oxidase / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D013270:Stomach / Q000601:surgery; D013274:Stomach Neoplasms / Q000378:metabolism / Q000473:pathology / Q000601:surgery
chemical_list: D014408:Biomarkers, Tumor; D012333:RNA, Messenger; D008249:Protein-Lysine 6-Oxidase; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2