title: Tools to evaluate the conformation of protein products.
abstract: Production of recombinant proteins is a process intensively used in the research laboratory. In addition, the main biotechnology market products are recombinant proteins and monoclonal antibodies. The biological (and clinical) properties of the protein product strongly depend on the conformation of the polypeptide. Therefore, assessment of the correct conformation of the produced protein is crucial. There is no single method to assess every aspect of protein structure or function. Depending on the protein, the methods of choice vary. There are general methods to evaluate not only mass and primary sequence of the protein, but also higher-order structure. This review outlines the principal techniques for determining the conformation of a protein from structural (biophysical methods) to functional (in vitro binding assays) analyses.
authors.affiliation: Lab. Fisicoquímica Biológica, Instituto de Química Biológica, Facultad de Ciencias, Universidad de la República, Uruguay.
authors.forename: Bruno
authors.initials: B
authors.lastname: Manta
authors.forename: Gonzalo
authors.initials: G
authors.lastname: Obal
authors.forename: Alejandro
authors.initials: A
authors.lastname: Ricciardi
authors.forename: Otto
authors.initials: O
authors.lastname: Pritsch
authors.forename: Ana
authors.initials: A
authors.lastname: Denicola
mesh_terms: D001681:Biological Assay / Q000379:methods*; D001709:Biotechnology / Q000379:methods; D002152:Calorimetry, Differential Scanning; D002942:Circular Dichroism; D005453:Fluorescence; D011485:Protein Binding; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D020349:Surface Plasmon Resonance
chemical_list: D011994:Recombinant Proteins