title: ZFPIP/Zfp462 is involved in P19 cell pluripotency and in their neuronal fate.
abstract: The nuclear zinc finger protein ZFPIP/Zfp462 is an important factor involved in cell division during the early embryonic development of vertebrates. In pluripotent P19 cells, ZFPIP/Zfp462 takes part in cell proliferation, likely via its role in maintaining chromatin structure. To further define the function of ZFPIP/Zfp462 in the mechanisms of pluripotency and cell differentiation, we constructed a stable P19 cell line in which ZFPIP/Zfp462 knockdown is inducible. We report that ZFPIP/Zfp462 was vital for mitosis and self-renewal in pluripotent P19 cells. Its depletion induced substantial decreases in the expression of the pluripotency genes Nanog, Oct4 and Sox2 and was associated with the transient expression of specific neuronal differentiation markers. We also demonstrated that ZFPIP/Zfp462 expression appears to be unnecessary after neuronal differentiation is induced in P19 cells. Taken together, our results strongly suggest that ZFPIP/Zfp462 is a key chromatin factor involved in maintaining P19 pluripotency and in the early mechanisms of neural differentiation but that it is dispensable in differentiated P19 cells.
authors.affiliation: CNRS UMR 6061, Institut de Génétique et Développement de Rennes, Université de Rennes 1, Campus Villejean, 2 avenue du Professeur Léon Bernard, Rennes Cedex, France.
authors.forename: Julie
authors.initials: J
authors.lastname: Massé
authors.forename: Claire
authors.initials: C
authors.lastname: Piquet-Pellorce
authors.forename: Justine
authors.initials: J
authors.lastname: Viet
authors.forename: Daniel
authors.initials: D
authors.lastname: Guerrier
authors.forename: Isabelle
authors.initials: I
authors.lastname: Pellerin
authors.forename: Stéphane
authors.initials: S
authors.lastname: Deschamps
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D004268:DNA-Binding Proteins; D051379:Mice; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000166:cytology* / Q000378:metabolism*; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D009419:Nerve Tissue Proteins; C519662:Zfp462 protein, mouse