title: Development and validation of a potentiometric biosensor assay for tylosin with demonstrated applicability for the detection of two other antimicrobial growth-promoter compounds in feedstuffs.
abstract: A potentiometric biosensor assay based on a commercially available polyclonal antibody was developed to detect tylosin residues in animal feed. The method can be used as a rapid (less than 45 min) laboratory-based procedure or as a portable field-test for the simultaneous measurement of up to 12 different samples. For both procedures the qualitative detection capability (CCβ) for tylosin was determined as 0.2 mg kg(-1) in a range of animal feeds with a measurement repeatability at concentrations between 0.2 and 4 mg kg(-1) of ≤13% coefficient of variation (%CV). The field-test format was capable of detecting tylosin residues at operating (external air) temperatures ranging between +4 and 37°C, although some reduction in signal was observed at the lower temperatures. The laboratory-based tylosin assay was evaluated using 16 medicated and 22 non-medicated feeds and was found to give comparable data with a confirmatory method based upon liquid chromatography-tandem mass spectrometry (LC-MS/MS). The potential to develop a multi-probe format assay for the simultaneous detection of tylosin, spiramycin and virginiamycin was also demonstrated. Cross-validation in a second laboratory showed the assay to be transferable, reliable and robust.
authors.affiliation: a The Food and Environment Research Agency (Fera) , Sand Hutton , York YO41 1LZ , UK.
authors.forename: S L
authors.initials: SL
authors.lastname: Stead
authors.forename: K B
authors.initials: KB
authors.lastname: Wolodko-Cierniak
authors.forename: S F
authors.initials: SF
authors.lastname: Richmond
authors.forename: M
authors.initials: M
authors.lastname: Sharman
authors.forename: P
authors.initials: P
authors.lastname: Driver
authors.forename: P
authors.initials: P
authors.lastname: Teale
authors.forename: O
authors.initials: O
authors.lastname: Leonardova
authors.forename: D
authors.initials: D
authors.lastname: Purvis
mesh_terms: D000821:Animal Feed / Q000032:analysis* / Q000633:toxicity; D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000633:toxicity; D015374:Biosensing Techniques / Q000379:methods*; D005506:Food Contamination / Q000032:analysis / Q000331:legislation & jurisprudence; D059022:Food Safety; D006133:Growth Substances / Q000032:analysis / Q000633:toxicity; D006801:Humans; D011199:Potentiometry / Q000379:methods; D015572:Spiramycin / Q000032:analysis / Q000633:toxicity; D015645:Tylosin / Q000032:analysis* / Q000633:toxicity; D019155:Veterinary Drugs / Q000032:analysis / Q000633:toxicity; D014769:Virginiamycin / Q000032:analysis / Q000633:toxicity
chemical_list: D000900:Anti-Bacterial Agents; D006133:Growth Substances; D019155:Veterinary Drugs; D014769:Virginiamycin; D015572:Spiramycin; D015645:Tylosin