title: A novel SNP of liver-type fatty acid-binding protein gene in duck and its associations with the intramuscular fat.
abstract: Liver-type fatty acid-binding protein (L-FABP) is a member of intracellular lipid-binding proteins involved in the transportation of fatty acids. We detected the polymorphisms of duck L-FABP gene and its association with the intramuscular fat (IMF) and other fat-related traits. The complete sequence of duck L-FABP gene (four exons and three introns, 2,542 bp) was obtained in this study. The polymorphism of L-FABP gene was examined with direct DNA sequencing method in 231 individuals from different breeds, and a novel single nucleotide polymorphism in the exon 3 was detected. The polymorphism was shown to be associated with the contents of C16:0, C18:3 and the total IMF in pectoral muscle. The content of C16:0 in genotype CC was significantly higher than CT (P < 0.01) and TT (P < 0.01), and the genotype CT was higher than TT (P < 0.01). The content of C18:3 in genotype TT was significantly higher than CC and CT (P < 0.01), whereas the genotype CC and CT had no significant difference (P > 0.05). The content of IMF in genotype CC was significantly higher than CT (P < 0.01). However, no significant difference was detected between genotype CC and TT or genotype CT and TT (P > 0.05).
authors.affiliation: College of Animal Sciences, Zhejiang University, No. 388 Yuhangtang Road, Hangzhou 310058, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: He
authors.forename: Jiucheng
authors.initials: J
authors.lastname: Chen
authors.forename: Lizhi
authors.initials: L
authors.lastname: Lu
authors.forename: Yong
authors.initials: Y
authors.lastname: Tian
authors.forename: Zhengrong
authors.initials: Z
authors.lastname: Tao
authors.forename: Deqian
authors.initials: D
authors.lastname: Wang
authors.forename: Jinjun
authors.initials: J
authors.lastname: Li
authors.forename: Guoqin
authors.initials: G
authors.lastname: Li
authors.forename: Junda
authors.initials: J
authors.lastname: Shen
authors.forename: Yan
authors.initials: Y
authors.lastname: Fu
authors.forename: Dong
authors.initials: D
authors.lastname: Niu
mesh_terms: D052436:Adipose Tissue, White / Q000502:physiology; D000818:Animals; D001483:Base Sequence; D001823:Body Composition / Q000235:genetics*; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D004372:Ducks / Q000235:genetics*; D050556:Fatty Acid-Binding Proteins / Q000235:genetics*; D056726:Genetic Association Studies; D008969:Molecular Sequence Data; D010369:Pectoralis Muscles / Q000502:physiology; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D050556:Fatty Acid-Binding Proteins