title: PERMANENT GENETIC RESOURCES: Twelve polymorphic microsatellite markers for the bonefish, Albula vulpes and two congeners.
abstract: Twelve polymorphic microsatellite loci were isolated for the bonefish, Albula vulpes using a polymerase chain reaction-based procedure. The number of alleles ranged from two to 23 (mean = 8.8) in 37 specimens from south Florida. Observed and expected heterozygosities ranged from 0.07 to 0.77 (mean = 0.42) and from 0.07 to 0.84 (mean = 0.48), respectively. There were no significant departures from Hardy-Weinberg equilibrium and no evidence of genotypic disequilibrium between any pair of loci. In a cross-amplification test, all markers yielded appropriately sized alleles for specimens of the provisional Albula sp. B and 11 of the 12 loci amplified for those of Albula glossodonta.
authors.affiliation: Florida Fish and Wildlife Research Institute, 100 Eighth Avenue S.E. Saint Petersburg, FL 33701-5095, USA.
authors.forename: Seifu
authors.initials: S
authors.lastname: Seyoum
authors.forename: Elizabeth M
authors.initials: EM
authors.lastname: Wallace
authors.forename: Michael D
authors.initials: MD
authors.lastname: Tringali