title: Low-temperature synthesis of nanoparticle-assembled, transparent, and low-crystallized hydroxyapatite blocks.
abstract: The conditions for preparing transparent blocks assembled with low-crystallized hydroxyapatite (HAp) nanoparticles were examined. An aqueous dispersion of 32-nm-sized HAp nanoparticles was prepared by a wet chemical process at room temperature (18-22 °C), and then the nanoparticle-assembled block was prepared by casting the dispersion at 60 °C. We also proposed a novel casting method on flowable substrates to fabricate crack-free nanoparticle-assembled blocks, because large and thick blocks were not obtained by a conventional casting method on solid substrates due to crack formation. The nanoparticle-assembled transparent HAp had nanosized pores among the particles. Cell adhesion and proliferation on the block could be directly observed with an optical microscope.
authors.affiliation: Department of Biomedical Engineering, School of Biology-Oriented Science and Technology, Kinki University, 930 Nishi-Mitani, Kinokawa, Wakayama 649-6493, Japan. okada-m@cc.osaka-dent.ac.jp
authors.forename: Masahiro
authors.initials: M
authors.lastname: Okada
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Furuzono
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D003460:Crystallization; D017886:Durapatite / Q000737:chemistry*; D005347:Fibroblasts / Q000166:cytology; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D013499:Surface Properties; D013696:Temperature
chemical_list: D017886:Durapatite