title: Comparison of three detergent-free protein extraction protocols for white adipose tissue.
abstract: A comparative study of three detergent-free protein extraction protocols--a differential centrifugal fractionation, a delipidation protocol based on the Bligh and Dyer method, and the trifluoroethanol addition as cosolvent to an aqueous buffer--was performed on white adipose tissue. The performance of the protocols directly compatible with liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) was evaluated based on the total protein extraction yield and the protein recovery from different functional and cellular compartments. The most suitable method for the extraction of white adipose tissue proteins from a wide range of cellular and structural compartments was the delipidation protocol based on the Bligh and Dyer method.
authors.affiliation: Life Sciences Mass Spectrometry, School of Pharmaceutical Sciences, University of Geneva, CH-1211 Geneva 4, Switzerland.
authors.forename: Tatjana
authors.initials: T
authors.lastname: Sajic
authors.forename: Gérard
authors.initials: G
authors.lastname: Hopfgartner
authors.forename: Ildiko
authors.initials: I
authors.lastname: Szanto
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Varesio
mesh_terms: D052436:Adipose Tissue, White / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western / Q000379:methods; D002498:Centrifugation / Q000379:methods*; D005591:Chemical Fractionation / Q000379:methods*; D002725:Chloroform / Q000737:chemistry; D003902:Detergents / Q000737:chemistry; D008297:Male; D000432:Methanol / Q000737:chemistry; D051379:Mice; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*; D013395:Sucrose / Q000737:chemistry; D014270:Trifluoroethanol / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D003902:Detergents; D011506:Proteins; D014867:Water; D013395:Sucrose; D014270:Trifluoroethanol; D002725:Chloroform; D000432:Methanol