title: [Action of an extract of Vanda coerulea on the senescence of skin fibroblasts].
abstract: The family of the orchids to date is poorly studied as a potential source of molecules with biological activity. The phytochemical analysis of extracts of Vanda coerulea stems (Orchidaceae), the isolation and the purification of the secondary metabolites realized by CLHP followed with high-resolution mass spectrometry and mono and two-dimensional nuclear magnetic resonance made it possible to identify the joint presence in an orchid of three stilbenoïds i.e, imbricatin, methoxycoelonin and gigantol. By flow cytometry, it is shown that the replicative senescence of human normal skin fibroblasts involves a reduction in the number of cells in phase S. A proteins chips technology dedicated to cell cycle proteins makes it possible to correlate this decrease of the number of cells in phase S to a decrease in cyclin E and cyclin dependant kinase 2, cdk2. The treatment by an ethanolic extract of stems of Vanda coerulea titrated in the three stilbenoids restores the percentage at an equivalent rate to that of young cells and the rate of cyclin E and, cdk2, thus bringing a beginning of explanation of their mechanism. These activities let predict an interesting potential as active ingredients to fight against the visible signs of cutaneous ageing.
authors.affiliation: LVMH recherche, Saint-Jean-de-Braye, France. fredericbonte@research.lvmh-pc.com
authors.forename: F
authors.initials: F
authors.lastname: Bonté
authors.forename: C
authors.initials: C
authors.lastname: Simmler
authors.forename: A
authors.initials: A
authors.lastname: Lobstein
authors.forename: F
authors.initials: F
authors.lastname: Pellicier
authors.forename: J-H
authors.initials: JH
authors.lastname: Cauchard
mesh_terms: D018797:Cell Cycle Proteins / Q000096:biosynthesis; D049109:Cell Proliferation / Q000187:drug effects; D016922:Cellular Senescence / Q000187:drug effects*; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D029595:Orchidaceae / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology; D018547:Plant Stems / Q000737:chemistry; D013267:Stilbenes / Q000737:chemistry
chemical_list: D018797:Cell Cycle Proteins; D010936:Plant Extracts; D013267:Stilbenes