title: Distributions of transposable elements reveal hazardous zones in mammalian introns.
abstract: Comprising nearly half of the human and mouse genomes, transposable elements (TEs) are found within most genes. Although the vast majority of TEs in introns are fixed in the species and presumably exert no significant effects on the enclosing gene, some markedly perturb transcription and result in disease or a mutated phenotype. Factors determining the likelihood that an intronic TE will affect transcription are not clear. In this study, we examined intronic TE distributions in both human and mouse and found several factors that likely contribute to whether a particular TE can influence gene transcription. Specifically, we observed that TEs near exons are greatly underrepresented compared to random distributions, but the size of these "underrepresentation zones" differs between TE classes. Compared to elsewhere in introns, TEs within these zones are shorter on average and show stronger orientation biases. Moreover, TEs in extremely close proximity (<20 bp) to exons show a strong bias to be near splice-donor sites. Interestingly, disease-causing intronic TE insertions show the opposite distributional trends, and by examining expressed sequence tag (EST) databases, we found that the proportion of TEs contributing to chimeric TE-gene transcripts is significantly higher within their underrepresentation zones. In addition, an analysis of predicted splice sites within human long terminal repeat (LTR) elements showed a significantly lower total number and weaker strength for intronic LTRs near exons. Based on these factors, we selectively examined a list of polymorphic mouse LTR elements in introns and showed clear evidence of transcriptional disruption by LTR element insertions in the Trpc6 and Kcnh6 genes. Taken together, these studies lend insight into the potential selective forces that have shaped intronic TE distributions and enable identification of TEs most likely to exert transcriptional effects on genes.
authors.affiliation: Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, British Columbia, Canada.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Mark T
authors.initials: MT
authors.lastname: Romanish
authors.forename: Dixie L
authors.initials: DL
authors.lastname: Mager
mesh_terms: D000818:Animals; D019295:Computational Biology; D003198:Computer Simulation; D004251:DNA Transposable Elements; D030541:Databases, Genetic; D051638:Ether-A-Go-Go Potassium Channels / Q000235:genetics; D020224:Expressed Sequence Tags; D006801:Humans; D007438:Introns; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008957:Models, Genetic; D016254:Mutagenesis, Insertional; D009154:Mutation; D012326:RNA Splicing; D050052:TRPC Cation Channels / Q000235:genetics; D000074287:TRPC6 Cation Channel; D020079:Terminal Repeat Sequences; D014158:Transcription, Genetic
chemical_list: D004251:DNA Transposable Elements; D051638:Ether-A-Go-Go Potassium Channels; D050052:TRPC Cation Channels; D000074287:TRPC6 Cation Channel; C492146:Trpc6 protein, mouse