title: Cross-reactivity of anti-EV71 IgM and neutralizing antibody in series sera of patients infected with Enterovirus 71 and Coxsackievirus A 16.
abstract: OBJECTIVE
  To evaluate the cross-reactivity of anti-EV71 IgM and neutralizing antibody in series sera of patients infected with EV71 and CA16.
  
  
  METHODS
  Real-time RT-PCR, virus isolation, ELISA and neutralization test were used to detect enteroviruses from clinical specimens and series sera of 79 HFMD patients.
  
  
  RESULTS
  27 EV71, 37 CA16, and 11 other enterovirus-infected patients were identified by RT-PCR. Among EV71 infected patients, anti-EV71 IgM positive ratios were 87.5% during 1-3 days after onset and 100% over 4 days after onset. In CA16 infected patients, the positive ratios were 7.4%, 26.4%, and 62.5% during 1-3 days, 4-6 days, and over 6 days after onset, respectively. Meanwhile, the results of neutralization test showed 18.9% of CA16 infected patients and 11.1% of EV71 infected patients present high cross-neutralization antibody against each other.
  
  
  CONCLUSIONS
  Cross-reactivity of anti-EV71 IgM in patients infected with EV71 and CA16 becomes stronger with the progress of disease. Moreover, high cross-neutralization antibody existing in part of patients suggests that the immune reactivity to EV71 infection can be recalled by CA16, and the immune reactivity to CA16 infection can be recalled by EV71. Therefore, identifying enteroviruses by neutralization test may not be an ideal selection.
authors.affiliation: Center of Clinical Laboratory, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, PR China.
authors.forename: Yulong
authors.initials: Y
authors.lastname: Lin
authors.forename: Kun
authors.initials: K
authors.lastname: Wen
authors.forename: Yuxian
authors.initials: Y
authors.lastname: Pan
authors.forename: Yadi
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Che
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood / Q000276:immunology*; D000937:Antigen-Antibody Reactions; D045744:Cell Line, Tumor; D002648:Child; D002675:Child, Preschool; D002522:Chlorocebus aethiops; D003429:Cross Reactions / Q000276:immunology; D004769:Enterovirus Infections / Q000276:immunology* / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay; D006232:Hand, Foot and Mouth Disease / Q000276:immunology* / Q000821:virology; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood / Q000276:immunology*; D007223:Infant; D009500:Neutralization Tests; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014709:Vero Cells
chemical_list: D057134:Antibodies, Neutralizing; D007075:Immunoglobulin M; D012367:RNA, Viral