title: Influence of inoculum density and aeration volume on biomass and bioactive compound production in bulb-type bubble bioreactor cultures of Eleutherococcus koreanum Nakai.
abstract: This study deals with the effects of initial inoculum density and aeration volume on biomass and bioactive compound production in adventitious roots of Eleutherococcus koreanum Nakai in bulb-type bubble bioreactors (3-L capacity). While the fresh and dry weights of the roots increased with increasing inoculum density, the highest percentage dry weight and accumulation of total target compounds (eleutheroside B and E, chlorogenic acid, total phenolics, and flavonoids) were noted at an inoculum density of 5.0 g L(-1). Poor aeration volume (0.05 vvm) stunted root growth, and high aeration volume (0.4 vvm) caused physiological disorders. Moreover, an inoculum density of 5.0 g L(-1) and an aeration volume of 0.1 vvm resulted in the highest concentration of total target compounds and least root death. Such optimization of culture conditions will be beneficial for the large-scale production of E. koreanum biomass and bioactive compounds.
authors.affiliation: CBN PLANTECH Co. Ltd., Industry Academic Cooperation Foundation Agribusiness Incubator Center 206, Chungbuk National University, Cheongju 361-763, Republic of Korea.
authors.forename: Eun-Jung
authors.initials: EJ
authors.lastname: Lee
authors.forename: Sang-Hyun
authors.initials: SH
authors.lastname: Moh
authors.forename: Kee-Yoeup
authors.initials: KY
authors.lastname: Paek
mesh_terms: D000332:Aerobiosis; D001688:Biological Products / Q000096:biosynthesis*; D018533:Biomass; D019149:Bioreactors; D002245:Carbon Dioxide / Q000032:analysis; D028001:Eleutherococcus / Q000166:cytology* / Q000254:growth & development*; D010100:Oxygen / Q000032:analysis; D018517:Plant Roots / Q000254:growth & development
chemical_list: D001688:Biological Products; D002245:Carbon Dioxide; D010100:Oxygen