title: Development and application of a simple loop-mediated isothermal amplification method on rapid detection of Listeria monocytogenes strains.
abstract: A loop-mediated isothermal amplification (LAMP) method for rapid detection of the food-borne L. monocytogenes strains had been developed and evaluated in this study. The optimal reaction condition was 65 °C for 45 min, with the detection limit as 1 pg DNA/tube and 100 CFU/reaction. Application of the established LAMP assay was performed on 182 food-borne L. monocytogenes strains using a rapid procedure and easy result confirmation, with the sensitivity of LAMP versus PCR assays as 96.7% (176/182) and 91.2% (166/182), respectively; with 100% specificity, positive predictive value (PPV) and negative predictive value (NPV) for both assays.
authors.affiliation: Food Safety Key Laboratory of Guangdong Province, College of Food Science, South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Yue
authors.initials: Y
authors.lastname: Li
authors.forename: Jin
authors.initials: J
authors.lastname: Chu
authors.forename: Zhenbo
authors.initials: Z
authors.lastname: Xu
authors.forename: Qingping
authors.initials: Q
authors.lastname: Zhong
mesh_terms: D017931:DNA Primers / Q000235:genetics; D005504:Food Analysis / Q000379:methods*; D005516:Food Microbiology; D008089:Listeria monocytogenes / Q000235:genetics / Q000302:isolation & purification*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers