title: [Effect of miR-146a on IL-18 expression in mouse macrophage].
abstract: OBJECTIVE
  To investigate the effect of miR-146a on the Th1/Th2 cytokine expression in mouse RAW264.7 cell line and primary peritoneal macrophage.
  
  
  METHODS
  miR-146a mimics, mimics negative control (NC mimics), inhibitor miR-146a and inhibitor negative control (NC inhibitor) were transfected into RAW264.7 cells and freshly isolated peritoneal macrophage. IL-18, IL-5 and IL-10 expressions in the cells were measured by real time PCR.
  
  
  RESULTS
  MiR-146a mimics suppressed IL-18 expression (P<0.05), and miR-146a specific inhibitor increased IL-18 expression significantly (P<0.05). However, IL-5 and IL-10 expressions were not affected by both miR-146a mimics and miR-146a inhibitor transfections.
  
  
  CONCLUSIONS
  These data demonstrate at first time that miR-146a can regulate Th1 cytokine IL-18 expression, but not affect Th2 cytokine IL-5 and IL-10 expressions.
authors.affiliation: National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China. ilovefreyal1984@yahoo.com.cn
authors.forename: Yang
authors.initials: Y
authors.lastname: Xiang
authors.forename: Ming-hong
authors.initials: MH
authors.lastname: Jiang
authors.forename: Dong-sheng
authors.initials: DS
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Gao
authors.forename: Ya-xi
authors.initials: YX
authors.lastname: Zhang
authors.forename: Yan-xin
authors.initials: YX
authors.lastname: Liu
authors.forename: De-xian
authors.initials: DX
authors.lastname: Zheng
mesh_terms: D000818:Animals; D002460:Cell Line; D020382:Interleukin-18 / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D017737:Macrophages, Peritoneal / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D014162:Transfection
chemical_list: D020382:Interleukin-18; D035683:MicroRNAs; C534190:Mirn146 microRNA, mouse