title: ELISA kit for mustard protein determination: interlaboratory study.
abstract: An interlaboratory study in 12 laboratories was performed to prove the validation of the ELISA method developed for the quantitative determination of mustard protein in foods. The ELISA kit used for this study is based on rabbit polyclonal antibody. This kit did not produce any false-positive results or cross-reactivity with in-house validation for a broad range of food matrixes with no detectable mustard protein. All participants obtained the Mustard ELISA kit with standard operational procedures, a list of samples, samples, and a protocol for recording test results. The study included 15 food samples and two spiked samples. Seven food matrix samples of zero mustard content and four samples with mustard declared as an ingredient showed mustard protein content lower than that of the first standard (0.42 mg/kg). Four samples with mustard declared as an ingredient revealed mustard protein content above 12.5 mg/kg (the highest standard). The statistical tests (Cochran, Dixon, and Mandel) and analysis of variance were used to evaluate the interlaboratory study results. Repeatability and reproducibility limits, as well as an LOQ (1.8 mg mustard proteins/kg) and LOD (0.5 mg mustard proteins/kg), for the kit were calculated.
authors.affiliation: Czech Agriculture and Food Inspection Authority, Za Opravnou 300/6, 150 06 Praha 5, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Cuhra
authors.forename: Dana
authors.initials: D
authors.lastname: Gabrovská
authors.forename: Jana
authors.initials: J
authors.lastname: Rysová
authors.forename: Petr
authors.initials: P
authors.lastname: Hanák
authors.forename: Frantisek
authors.initials: F
authors.lastname: Stumr
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D009149:Mustard Plant / Q000737:chemistry*; D010940:Plant Proteins / Q000378:metabolism*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D010940:Plant Proteins