title: Microfluidic reactors for diagnostics applications.
abstract: Diagnostic assays are an important part of health care, both in the clinic and in research laboratories. In addition to improving treatments and clinical outcomes, rapid and reliable diagnostics help track disease epidemiology, curb infectious outbreaks, and further the understanding of chronic illness. Disease markers such as antigens, RNA, and DNA are present at low concentrations in biological samples, such that the majority of diagnostic assays rely on an amplification reaction before detection is possible. Ideally, these amplification reactions would be sensitive, specific, inexpensive, rapid, integrated, and automated. Microfluidic technology currently in development offers many advantages over conventional benchtop reactions that help achieve these goals. The small reaction volumes and energy consumption make reactions cheaper and more efficient in a microfluidic reactor. Additionally, the channel architecture could be designed to perform multiple tests or experimental steps on one integrated, automated platform. This review explores the current research on microfluidic reactors designed to aid diagnostic applications, covering a broad spectrum of amplification techniques and designs.
authors.affiliation: Center for Biomedical Engineering, School of Engineering and Medical Sciences, Brown University, Providence, RI 02912, USA.
authors.forename: Stephanie E
authors.initials: SE
authors.lastname: McCalla
authors.forename: Anubhav
authors.initials: A
authors.lastname: Tripathi
mesh_terms: D000941:Antigens / Q000032:analysis; D015415:Biomarkers / Q000032:analysis*; D004247:DNA / Q000032:analysis; D004867:Equipment Design; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D044085:Microfluidics / Q000295:instrumentation* / Q000379:methods; D016133:Polymerase Chain Reaction / Q000295:instrumentation; D012313:RNA / Q000032:analysis; D021161:Self-Sustained Sequence Replication / Q000295:instrumentation
chemical_list: D000941:Antigens; D015415:Biomarkers; D012313:RNA; D004247:DNA