title: Erythrocyte sedimentation rate measurement by VES Matic Cube 80 in relation to inflammation plasma proteins.
abstract: Westergren method is considered as the reference procedure to measure Erythrocyte Sedimentation Rate (ESR) by the International Council for Standardization in Haematology. However, a closed automated method, VES Matic Cube 80 (DIESSE S.p.A., Siena, Italy), has been introduced as a new ESR measurement instrument. In this article, we report two different studies: first, we compared the two methods (Westergren and VES Matic Cube 80) and second, we correlated the inflammatory state of 248 patients with their ESR values. Total protein, albumin, C-reactive protein, and other inflammatory proteins were detected in each sample. The results obtained using VES Matic Cube 80 demonstrated a good correlation with those obtained using the Westergren method (Ordinary linear regression: y=0.955x-0.205, r(2) =0.816, P<0.05; Passing-Bablock regression equation: y=0.9153x-0.5763; Bland-Altman analysis: bias 1.2; limits of agreement -17.4-19.9) and with the inflammatory protein levels (CRP: r=0.554 and r=0.498 and Fibrinogen: r=0.699 and r=0.663 for Ves Matic Cube 80 and Westergren, respectively), supporting the hypothesis that VES Matic Cube 80 offers a fast and safe ESR determination, ensuring precision and a very good correlation with the reference method.
authors.affiliation: Dipartimento di Medicina Interna Scienze Endocrino-Metaboliche e Biochimica, Università di Siena, Siena, Italy. cerutti3@unisi.it
authors.forename: Helena
authors.initials: H
authors.lastname: Cerutti
authors.forename: Chiara
authors.initials: C
authors.lastname: Muzzi
authors.forename: Roberto
authors.initials: R
authors.lastname: Leoncini
authors.forename: Carlo
authors.initials: C
authors.lastname: Scapellato
authors.forename: Alessio
authors.initials: A
authors.lastname: Cortelazzo
authors.forename: Emiliano
authors.initials: E
authors.lastname: Furlani
authors.forename: Roberto
authors.initials: R
authors.lastname: Guerranti
mesh_terms: D001798:Blood Proteins / Q000378:metabolism*; D001799:Blood Sedimentation; D005260:Female; D006403:Hematologic Tests / Q000295:instrumentation* / Q000379:methods*; D006801:Humans; D007249:Inflammation / Q000097:blood*; D016014:Linear Models; D008297:Male; D008875:Middle Aged
chemical_list: D001798:Blood Proteins