title: Isolation and characterization of 11 microsatellite loci in the mangrove crab, Ucides cordatus (Ocypodidae: Ucidinae).
abstract: Eleven polymorphic microsatellite loci were described for the mangrove crab, Ucides cordatus, an important fishery resource on the Brazilian coast. The number of alleles observed at each locus varied between eight and 23. Observed and expected mean heterozygosities were 0.791 and 0.893 respectively. Amplification of all loci was highly successful, under the same polymerase chain reaction conditions. With the exception of P2D3, all loci adhered to the assumptions of the Hardy-Weinberg equilibrium and did not present deviations reflecting linkage disequilibrium. Given this, these markers will be extremely useful in future management programmes for U. cordatus stocks.
authors.affiliation: Instituto de Estudos Costeiros (IECOS), Campus de Bragança, UFPA, 68600-000 Bragança, PA, Brazil Laboratório de Polimorfismo de DNA, Departamento de Genética, UFPA, 66075-110 Belém, PA, Brazil Centro de Biologia Molecular e Engenharia Genética (CBMEG), UNICAMP, 13083-970 Campinas, SP, Brazil.
authors.forename: Eduardo Sousa
authors.initials: ES
authors.lastname: Varela
authors.forename: Evonnildo D Costa
authors.initials: ED
authors.lastname: Gonçalves
authors.forename: Melissa D Oliveira
authors.initials: MD
authors.lastname: Santos
authors.forename: Iracilda
authors.initials: I
authors.lastname: Sampaio
authors.forename: Horacio
authors.initials: H
authors.lastname: Schneider