title: Casein kinase 1 enables nucleus accumbens amphetamine-induced locomotion by regulating AMPA receptor phosphorylation.
abstract: The closely related δ and ε isoforms of the serine/threonine protein kinase casein kinase 1 (Csnk1) have been implicated in the generation of psychostimulant-induced behaviors. In this study, we show that Csnk1δ/ε produces its effects on behavior by acting on the Darpp-32-PP1 signaling pathway to regulate AMPA receptor phosphorylation in the nucleus accumbens (NAcc). Inhibiting Csnk1δ/ε in the NAcc with the selective inhibitor PF-670462 blocks amphetamine induced locomotion and its ability to increase phosphorylation of Darpp-32 at S137 and T34, decrease PP1 activity and increase phosphorylation of the AMPA receptor subunit at S845. Consistent with these findings, preventing GluR1 phosphorylation with the alanine mutant GluR1(S845A) reduces glutamate-evoked currents in cultured medium spiny neurons and blocks the locomotor activity produced by NAcc amphetamine. Thus, Csnk1 enables the locomotor and likely the incentive motivational effects of amphetamine by regulating Darrp-32-PP1-GlurR1(S845) signaling in the NAcc. As such, Csnk1 may be a critical target for intervention in the treatment of drug use disorders.
authors.affiliation: Department of Psychiatry and Behavioral Neuroscience, The University of Chicago, Chicago, Illinois 60637, USA.
authors.forename: Dongdong
authors.initials: D
authors.lastname: Li
authors.forename: Stacy
authors.initials: S
authors.lastname: Herrera
authors.forename: Nancy
authors.initials: N
authors.lastname: Bubula
authors.forename: Elena
authors.initials: E
authors.lastname: Nikitina
authors.forename: Abraham A
authors.initials: AA
authors.lastname: Palmer
authors.forename: Dorothy A
authors.initials: DA
authors.lastname: Hanck
authors.forename: Jessica A
authors.initials: JA
authors.lastname: Loweth
authors.forename: Paul
authors.initials: P
authors.lastname: Vezina
mesh_terms: D000661:Amphetamine / Q000494:pharmacology*; D000818:Animals; D048149:Casein Kinase 1 epsilon / Q000502:physiology*; D048148:Casein Kinase Idelta / Q000502:physiology*; D018698:Glutamic Acid / Q000502:physiology; D008297:Male; D009043:Motor Activity / Q000187:drug effects / Q000502:physiology*; D009714:Nucleus Accumbens / Q000187:drug effects / Q000502:physiology*; D010766:Phosphorylation / Q000502:physiology; D020033:Protein Isoforms / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D018091:Receptors, AMPA / Q000378:metabolism* / Q000502:physiology
chemical_list: D000661:Amphetamine; D048149:Casein Kinase 1 epsilon; D048148:Casein Kinase Idelta; D018698:Glutamic Acid; D020033:Protein Isoforms; D018091:Receptors, AMPA