title: Identification of Transcription Factor-DNA Interactions In Vivo.
abstract: Recent technological developments have revolutionized our understanding of transcriptional regulation by providing an unprecedented ability to interrogate in vivo transcription factor binding. The combination of high-throughput sequencing with chromatin precipitation of transcription factors and specifically labeled histones has allowed direct protein-DNA contacts to be visualized across genomes as large and complex as mammals at base-pair resolution. This chapter reviews the developments that led to these insights, with particular focus on examples of early protein-DNA localization experiments using genomic microarrays in mammals and yeast. Four state-of-the-art research directions are highlighted as examples of previously unimaginable frontiers now under active investigation.
authors.affiliation: Cancer Research UK, Li Ka Shing Centre, University of Cambridge, Cambridge, UK, duncan.odom@cancer.org.uk.
authors.forename: Duncan T
authors.initials: DT
authors.lastname: Odom
mesh_terms: D000818:Animals; D002843:Chromatin; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics; D006657:Histones / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics
chemical_list: D002843:Chromatin; D004268:DNA-Binding Proteins; D006657:Histones; D014157:Transcription Factors; D004247:DNA