title: [Pathologic and molecular genetic study of anaplastic lymphoma kinase-positive large B-cell lymphoma].
abstract: OBJECTIVE
  To study clinicopathologic and genetic features of anaplastic lymphoma kinase (ALK)-positive large B-cell lymphoma (LBCL).
  
  
  METHODS
  Light microscopy, EliVision immunohistocheimical method and fluorescence in-situ hybridization were used to evaluate three ALK + LBCL cases recently diagnosed accompanied with a literature review.
  
  
  RESULTS
  All three cases were male adult patients (mean age = 36.3 years) with nodal involvement by lymphoma. Histologic evaluation revealed a diffuse effacement of the nodal architecture by the infiltration of tumor cells. Sinusoidal infiltration was seen. The neoplastic cells were large and exhibited the immunoblastic/plasmablastic morphology. By immunohistochemistry, all the cases showed a cytoplasmic granular staining of ALK. They were positive for CD45, CD138, and epithelial membrane antigen (EMA), but were negative for CD3, CD20, CD79a and CD30. Fluorescence in situ hybridization (FISH) demonstrated the presence of ALK gene translocation in all of the cases.
  
  
  CONCLUSIONS
  ALK + LBCL represents a distinct variant of diffuse large B-cell lymphoma, usually involving lymph node of middle-aged men. The tumor has a immunoblastic/plasmablastic morphology along with a distinct immunophenotypic profile and ALK gene rearrangement.
authors.affiliation: Department of Pathology, Fujian Provincial Cancer Hospital, Fuzhou 350014, China.
authors.forename: Rong-fang
authors.initials: RF
authors.lastname: Huang
authors.forename: Gang
authors.initials: G
authors.lastname: CHEN
authors.forename: Li-ping
authors.initials: LP
authors.lastname: GONG
authors.forename: Li-li
authors.initials: LL
authors.lastname: LU
mesh_terms: D051997:ADP-ribosyl Cyclase 1 / Q000378:metabolism; D000328:Adult; D000077548:Anaplastic Lymphoma Kinase; D003937:Diagnosis, Differential; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D017493:Leukocyte Common Antigens / Q000378:metabolism; D016403:Lymphoma, Large B-Cell, Diffuse / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D017728:Lymphoma, Large-Cell, Anaplastic / Q000378:metabolism / Q000473:pathology; D008297:Male; D018396:Mucin-1 / Q000378:metabolism; D009101:Multiple Myeloma / Q000378:metabolism / Q000473:pathology; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics* / Q000378:metabolism*; D014178:Translocation, Genetic
chemical_list: D018396:Mucin-1; C000626173:ALK protein, human; D000077548:Anaplastic Lymphoma Kinase; D020794:Receptor Protein-Tyrosine Kinases; D017493:Leukocyte Common Antigens; D051997:ADP-ribosyl Cyclase 1