title: α-Cyclodextrin enhances myoblast fusion and muscle differentiation by the release of IL-4.
abstract: Muscle fibers are formed during embryonic development by the fusion of mononucleated myoblasts. The spatial structure and molecular composition of the sarcolemma are crucial for the myoblast recognition and fusion steps. Cyclodextrins are a group of substances that have the ability to solubilize lipids through the formation of molecular inclusion complexes. Previously, we have shown that methyl-β-cyclodextrin (MbCD) enhances muscle differentiation. Here, we analyzed the effects of α-cyclodextrin (aCD) during myogenesis. Myogenic cultures treated with aCD showed an increase in myoblast fusion and in the expression of myogenin, sarcomeric tropomyosin and desmin. aCD-conditioned media accelerates myogenesis in a similar way as aCD does, and increased levels of IL-4 were found in aCD-conditioned media. aCD-induced effects on myogenesis were inhibited by an anti-IL4 antibody. These results show that α-cyclodextrin induces myogenic differentiation by the release of IL-4.
authors.affiliation: Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21949-590, Brazil.
authors.forename: Ana Claudia Batista
authors.initials: AC
authors.lastname: Possidonio
authors.forename: Mariana Lopes
authors.initials: ML
authors.lastname: Senna
authors.forename: Debora Morueco
authors.initials: DM
authors.lastname: Portilho
authors.forename: Carolina
authors.initials: C
authors.lastname: Pontes Soares
authors.forename: Luzia
authors.initials: L
authors.lastname: da Silva Sampaio
authors.forename: Marcelo
authors.initials: M
authors.lastname: Einicker-Lamas
authors.forename: Morgana Teixeira Lima
authors.initials: MT
authors.lastname: Castelo Branco
authors.forename: Manoel Luis
authors.initials: ML
authors.lastname: Costa
authors.forename: Claudia
authors.initials: C
authors.lastname: Mermelstein
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002459:Cell Fusion; D002478:Cells, Cultured; D002642:Chick Embryo; D017077:Culture Media, Conditioned / Q000737:chemistry / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism*; D024510:Muscle Development / Q000187:drug effects; D018482:Muscle, Skeletal / Q000166:cytology / Q000187:drug effects / Q000196:embryology; D032446:Myoblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism* / Q000502:physiology*; D047391:alpha-Cyclodextrins / Q000494:pharmacology*
chemical_list: D017077:Culture Media, Conditioned; D047391:alpha-Cyclodextrins; D015847:Interleukin-4; C032613:alpha-cyclodextrin