title: Characterization of 24 microsatellite loci in delta smelt, Hypomesus transpacificus, and their cross-species amplification in two other smelt species of the Osmeridae family.
abstract: We characterized 24 polymorphic tetranucleotide microsatellite loci for delta smelt (Hypomesus transpacificus) endemic to the San Francisco Bay Estuary, CA, USA. Screening of samples (n = 30) yielded two to 26 alleles per locus with observed levels of heterozygosity ranging from 0.17 to 1.0. Only one locus deviated from Hardy-Weinberg equilibrium, suggesting these individuals originate from a single panmictic population. Linkage disequilibrium was found in two pairs of loci after excluding the locus out of Hardy-Weinberg equilibrium. Twenty-two primer pairs cross-amplified in wakasagi smelt (Hypomesus nipponensis), and 15 primer pairs cross-amplified in longfin smelt (Spirinchus thaleichthys).
authors.affiliation: Department of Animal Science, University of California - Davis, 1 Shields Avenue, Davis, CA 95616, USA.
authors.forename: Kathleen M
authors.initials: KM
authors.lastname: Fisch
authors.forename: Jessica L
authors.initials: JL
authors.lastname: Petersen
authors.forename: Melinda R
authors.initials: MR
authors.lastname: Baerwald
authors.forename: John K
authors.initials: JK
authors.lastname: Pedroia
authors.forename: Bernie
authors.initials: B
authors.lastname: May