title: Retinoic Acid induces loss of viral-DNA, phenotypic reversion and transformation-resistance of bovine papillomavirus type-1 DNA-transformed cells.
abstract: Two revertant cell lines were obtained by treatment of bovine papillomavirus type 1 (BPV-1) DNA-transformed cell lines B3 and BF3 with 5 muM of all-trans-retinoic acid (RA) for 10 weeks. The revertant cells were devoid of detectable viral DNA, no longer exhibited a transformed morphology and lost the ability to form multilayered transformed foci. They replicated with a population doubling time of 16-18 hours, which was also characteristic of the C127, B3 and BF3 cells. They attained a similar saturation density as the C127 cells. However, the revertant cells were at least 5- to 7-fold more resistant than the parental C127 cells to neoplastic transformation induced by BPV-1 DNA.
authors.affiliation: BRITISH COLUMBIA CANC RES CTR,ENVIRONM CARCINOGENESIS SECT,601 W 10TH AVE,VANCOUVER V5Z 1L3,BC,CANADA.
authors.forename: G
authors.initials: G
authors.lastname: Li
authors.forename: S
authors.initials: S
authors.lastname: Tsang
authors.forename: P
authors.initials: P
authors.lastname: Lam
authors.forename: H
authors.initials: H
authors.lastname: Stich