title: In-line monitoring of extraction process of scutellarein from Erigeron breviscapus (vant.) Hand-Mazz based on qualitative and quantitative uses of near-infrared spectroscopy.
abstract: The application of near-infrared (NIR) spectroscopy for in-line monitoring of extraction process of scutellarein from Erigeron breviscapus (vant.) Hand-Mazz was investigated. For NIR measurements, two fiber optic probes designed to transmit NIR radiation through a 2 mm pathlength flow cell were utilized to collect spectra in real-time. High performance liquid chromatography (HPLC) was used as a reference method to determine scutellarein in extract solution. Partial least squares regression (PLSR) calibration model of Savitzky-Golay smoothing NIR spectra in the 5450-10,000 cm(-1) region gave satisfactory predictive results for scutellarein. The results showed that the correlation coefficients of calibration and cross validation were 0.9967 and 0.9811, respectively, and the root mean square error of calibration and cross validation were 0.044 and 0.105, respectively. Furthermore, both the moving block standard deviation (MBSD) method and conformity test were used to identify the end point of extraction process, providing real-time data and instant feedback about the extraction course. The results obtained in this study indicated that the NIR spectroscopy technique provides an efficient and environmentally friendly approach for fast determination of scutellarein and end point control of extraction process.
authors.affiliation: College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Yongjiang
authors.initials: Y
authors.lastname: Wu
authors.forename: Ye
authors.initials: Y
authors.lastname: Jin
authors.forename: Haiying
authors.initials: H
authors.lastname: Ding
authors.forename: Lianjun
authors.initials: L
authors.lastname: Luan
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
authors.forename: Xuesong
authors.initials: X
authors.lastname: Liu
mesh_terms: D047310:Apigenin / Q000032:analysis* / Q000302:isolation & purification*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D049919:Erigeron / Q000737:chemistry*; D016018:Least-Squares Analysis; D010936:Plant Extracts / Q000032:analysis*; D019265:Spectroscopy, Near-Infrared
chemical_list: D010936:Plant Extracts; D047310:Apigenin; C458179:scutellarein