title: Critical role of c-Jun N-terminal kinase in regulating bFGF-induced angiogenesis in vitro.
abstract: Angiogenesis, the process of new blood vessels formation, is a critical step for wound healing, tumour growth and metastasis, diabetic retinopathy, psoriasis, etc. The present study was designed to investigate whether c-Jun N-terminal kinase/stress-activated protein kinase (JNK/SAPK) is critical for regulating basic fibroblastic growth factor (bFGF)-induced angiogenesis in human umbilical vein endothelial cells (HUVECs). Our results showed that bFGF-induced HUVECs proliferation, migration and tube formation with a concentration-dependent manner. Further results showed that bFGF induced the phosphorylation of JNK/SAPK at 15 min. Both JNK/SAPK inhibitor SP600125 and JNK/SAPK peptide inhibitor 420116 could inhibit bFGF-induced HUVECs proliferation, migration and tube formation, so did JNK/SAPK-specific siRNA. Moreover, when HUVECs were stimulated with bFGF, upstream signals of JNK/SAPK, SEK1/MKK4 and MKK7 were both activated at 2 min. In summary, our results indicate that JNK/SAPK signal pathway plays an important role in regulating bFGF-mediated angiogenesis in HUVECs, which may therefore be a new therapeutic approach for the treatment of angiogenesis-associated diseases.
authors.affiliation: The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201210, China.
authors.forename: Shen
authors.initials: S
authors.lastname: Kaikai
authors.forename: Sheng
authors.initials: S
authors.lastname: Yuchen
authors.forename: Ji
authors.initials: J
authors.lastname: Lili
authors.forename: Wang
authors.initials: W
authors.lastname: Zhengtao
mesh_terms: D000873:Anthracenes / Q000494:pharmacology; D002465:Cell Movement; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D020868:Gene Silencing; D006801:Humans; D048056:Mitogen-Activated Protein Kinase 9 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D018919:Neovascularization, Physiologic; D034741:RNA, Small Interfering / Q000235:genetics; D015398:Signal Transduction; D014471:Umbilical Veins / Q000166:cytology
chemical_list: D000873:Anthracenes; D034741:RNA, Small Interfering; D016222:Fibroblast Growth Factor 2; C432165:pyrazolanthrone; D048056:Mitogen-Activated Protein Kinase 9