title: Improved COI barcoding primers for Southeast Asian perching birds (Aves: Passeriformes).
abstract: The All Birds Barcoding Initiative aims to assemble a DNA barcode database for all bird species, but the 648-bp 'barcoding' region of cytochrome c oxidase subunit I (COI) can be difficult to amplify in Southeast Asian perching birds (Aves: Passeriformes). Using COI sequences from complete mitochondrial genomes, we designed a primer pair that more reliably amplifies and sequences the COI barcoding region of Southeast Asian passerine birds. The 655-bp region amplified with these primers overlaps the COI region amplified with other barcoding primer pairs, enabling direct comparison of sequences with previously published DNA barcodes.
authors.affiliation: Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543, Division of Zoology, Research Centre for Biology-LIPI, Jl. Raya Bogor Km 46, Cibinong-Bogor, 16911, Indonesia.
authors.forename: David J
authors.initials: DJ
authors.lastname: Lohman
authors.forename: Dewi M
authors.initials: DM
authors.lastname: Prawiradilaga
authors.forename: Rudolf
authors.initials: R
authors.lastname: Meier