title: Structure-function analysis of a CVNH-LysM lectin expressed during plant infection by the rice blast fungus Magnaporthe oryzae.
abstract: The rice blast fungus Magnaporthe oryzae's genome encodes a hypothetical protein (MGG_03307) containing a type III CVNH lectin, in which a LysM domain is inserted between individual repeats of a single CVNH domain. At present, no structural or ligand binding data are available for any type III CVNH and functional studies in natural source organisms are scarce. Here, we report NMR solution structure and functional data on MGG_03307. The structure of the CVNH/LysM module revealed that intact and functionally competent CVNH and LysM domains are present. Using NMR titrations, carbohydrate specificities for both domains were determined, and it was found that each domain behaves as an isolated unit without any interdomain communication. Furthermore, live-cell imaging revealed a predominant localization of MGG_03307 within the appressorium, the specialized fungal cell for gaining entry into rice tissue. Our results suggest that MGG_03307 plays a role in the early stages of plant infection.
authors.affiliation: Department of Structural Biology, University of Pittsburgh School of Medicine, Biomedical Science Tower 3, Pittsburgh, PA 15260, USA.
authors.forename: Leonardus M I
authors.initials: LM
authors.lastname: Koharudin
authors.forename: Arturo R
authors.initials: AR
authors.lastname: Viscomi
authors.forename: Barbara
authors.initials: B
authors.lastname: Montanini
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Kershaw
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Talbot
authors.forename: Simone
authors.initials: S
authors.lastname: Ottonello
authors.forename: Angela M
authors.initials: AM
authors.lastname: Gronenborn
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli; D005656:Fungal Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D037102:Lectins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D020082:Magnaporthe / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism*; D012275:Oryza / Q000382:microbiology; D010935:Plant Diseases / Q000382:microbiology; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D013329:Structure-Activity Relationship
chemical_list: D005656:Fungal Proteins; D037102:Lectins; D009844:Oligosaccharides; D011994:Recombinant Proteins