title: Comparative proteomics analysis of proteins expressed in the I-1 and I-2 internodes of strawberry stolons.
abstract: BACKGROUND
  Strawberries (Fragaria ananassa) reproduce asexually through stolons, which have strong tendencies to form adventitious roots at their second node. Understanding how the development of the proximal (I-1) and distal (I-2) internodes of stolons differ should facilitate nursery cultivation of strawberries.
  
  
  RESULTS
  Herein, we compared the proteomic profiles of the strawberry stolon I-1 and I-2 internodes. Proteins extracted from the internodes were separated by two-dimensional gel electrophoresis, and 164 I-1 protein spots and 200 I-2 protein spots were examined further. Using mass spectrometry and database searches, 38 I-1 and 52 I-2 proteins were identified and categorized (8 and 10 groups, respectively) according to their cellular compartmentalization and functionality. Many of the identified proteins are enzymes necessary for carbohydrate metabolism and photosynthesis. Furthermore, identification of proteins that interact revealed that many of the I-2 proteins form a dynamic network during development. Finally, given our results, we present a mechanistic scheme for adventitious root formation of new clonal plants at the second node.
  
  
  CONCLUSIONS
  Comparative proteomic analysis of I-1 and I-2 proteins revealed that the ubiquitin-proteasome pathway and sugar-hormone pathways might be important during adventitious root formation at the second node of new clonal plants.
authors.affiliation: Laboratory of Plant Molecular Biology and Proteomics, Institute of Biology, Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China. hzhsma@163.com.
authors.forename: Xianping
authors.initials: X
authors.lastname: Fang
authors.forename: Huasheng
authors.initials: H
authors.lastname: Ma
authors.forename: Dezhao
authors.initials: D
authors.lastname: Lu
authors.forename: Hong
authors.initials: H
authors.lastname: Yu
authors.forename: Wenguo
authors.initials: W
authors.lastname: Lai
authors.forename: Songlin
authors.initials: S
authors.lastname: Ruan