title: Polymerase chain reaction multiplexing of microsatellites and single nucleotide polymorphism markers for quantitative trait loci mapping of wild house mice.
abstract: We tested 96 microsatellites and 10 single nucleotide polymorphisms for their allelic distribution in two subspecies of the house mouse, Mus musculus musculus and M. m. domesticus. Sixty-two microsatellites discriminated strain-specific differences among nine wild-derived 'musculus' and 'domesticus' and three 'classical' laboratory strains. For efficient genotyping, we optimized multiplex conditions using five microsatellites per polymerase chain reaction. All 10 single nucleotide polymorphisms were also optimized for simultaneous analysis in one reaction using SNaPshot multiplex. The uniform distribution of markers on autosomes and on the X chromosome makes these panels potentially useful tools for quantitative trait loci mapping of wild house mice.
authors.affiliation: Mammal Research Institute, Polish Academy of Sciences, 17-230 Białowieża, Poland, Department of Population Biology, Institute of Vertebrate Biology, Academy of Sciences of the Czech Republic, 675 02 Studenec 122, Czech Republic.
authors.forename: Agata
authors.initials: A
authors.lastname: Kawałko
authors.forename: Petra
authors.initials: P
authors.lastname: Dufková
authors.forename: Jan M
authors.initials: JM
authors.lastname: Wójcik
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Piálek