title: Mechanical stretch increases CCN2/CTGF expression in anterior cruciate ligament-derived cells.
abstract: Anterior cruciate ligament (ACL)-to-bone interface serves to minimize the stress concentrations that would arise between two different tissues. Mechanical stretch plays an important role in maintaining cell-specific features by inducing CCN family 2/connective tissue growth factor (CCN2/CTGF). We previously reported that cyclic tensile strain (CTS) stimulates α1(I) collagen (COL1A1) expression in human ACL-derived cells. However, the biological function and stress-related response of CCN2/CTGF were still unclear in ACL fibroblasts. In the present study, CCN2/CTGF was observed in ACL-to-bone interface, but was not in the midsubstance region by immunohistochemical analyses. CTS treatments induced higher increase of CCN2/CTGF expression and secretion in interface cells compared with midsubstance cells. COL1A1 expression was not influenced by CCN2/CTGF treatment in interface cells despite CCN2/CTGF stimulated COL1A1 expression in midsubstance cells. However, CCN2/CTGF stimulated the proliferation of interface cells. Our results suggest that distinct biological function of stretch-induced CCN2/CTGF might regulate region-specific phenotypes of ACL-derived cells.
authors.affiliation: Department of Orthopaedic Surgery, Science of Functional Recovery and Reconstruction, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama, Japan.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Miyake
authors.forename: Takayuki
authors.initials: T
authors.lastname: Furumatsu
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kubota
authors.forename: Kazumi
authors.initials: K
authors.lastname: Kawata
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Ozaki
authors.forename: Masaharu
authors.initials: M
authors.lastname: Takigawa
mesh_terms: D000368:Aged; D016118:Anterior Cruciate Ligament / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D049109:Cell Proliferation; D002478:Cells, Cultured; D024042:Collagen Type I / Q000096:biosynthesis / Q000235:genetics; D000090602:Collagen Type I, alpha 1 Chain; D055513:Connective Tissue Growth Factor / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D013314:Stress, Mechanical
chemical_list: C526317:CCN2 protein, human; D024042:Collagen Type I; D000090602:Collagen Type I, alpha 1 Chain; D055513:Connective Tissue Growth Factor