title: Chromosomal position effects are linked to sir2-mediated variation in transcriptional burst size.
abstract: Gene expression noise varies with genomic position and is a driving force in the evolution of chromosome organization. Nevertheless, position effects remain poorly characterized. Here, we present a systematic analysis of chromosomal position effects by characterizing single-cell gene expression from euchromatic positions spanning the length of a eukaryotic chromosome. We demonstrate that position affects gene expression by modulating the size of transcriptional bursts, rather than their frequency, and that the histone deacetylase Sir2 plays a role in this process across the chromosome.
authors.affiliation: Ottawa Institute of Systems Biology, University of Ottawa, Ottawa, Ontario, Canada.
authors.forename: Cory
authors.initials: C
authors.lastname: Batenchuk
authors.forename: Simon
authors.initials: S
authors.lastname: St-Pierre
authors.forename: Lioudmila
authors.initials: L
authors.lastname: Tepliakova
authors.forename: Samyuktha
authors.initials: S
authors.lastname: Adiga
authors.forename: Anna
authors.initials: A
authors.lastname: Szuto
authors.forename: Nazir
authors.initials: N
authors.lastname: Kabbani
authors.forename: John C
authors.initials: JC
authors.lastname: Bell
authors.forename: Kristin
authors.initials: K
authors.lastname: Baetz
authors.forename: Mads
authors.initials: M
authors.lastname: Kærn
mesh_terms: D055012:Chromosomal Position Effects / Q000235:genetics*; D015825:Chromosomes, Fungal / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D008957:Models, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D038281:Silent Information Regulator Proteins, Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D056565:Sirtuin 2 / Q000235:genetics / Q000378:metabolism*; D013269:Stochastic Processes; D014158:Transcription, Genetic
chemical_list: D038281:Silent Information Regulator Proteins, Saccharomyces cerevisiae; C087778:SIR2 protein, S cerevisiae; D056565:Sirtuin 2