title: Characterization of a mutated p53 exhibiting a cell cycle-related nuclear translocation.
abstract: We studied the p53 expression in the F4NW0 cell line. We found that this mutated p53 has a deletion of eight amino acids, between the conserved domains IV and V. Only one of the two alleles is expressed. It contains a single base mutation, which seems to promote the use of a cryptic splicing acceptor site, resulting in the observed deletion. The protein shows a cell cycle-dependent nuclear translocation: in G(1), it is exclusively cytoplasmic, and during the G(1)/S transition, it translocates into the nucleus. Nevertheless, different antibodies assayed revealed different patterns of localization.
authors.affiliation: CEA,DEPT BIOL MOLEC & STRUCT,INSERM,U309,BIOL MOLEC CYCLE CELLULAIRE LAB,F-38054 GRENOBLE 9,FRANCE.
authors.forename: P
authors.initials: P
authors.lastname: Cahen
authors.forename: D
authors.initials: D
authors.lastname: Grunwald
authors.forename: D
authors.initials: D
authors.lastname: Pigott
authors.forename: M
authors.initials: M
authors.lastname: Brocard
authors.forename: S
authors.initials: S
authors.lastname: Khochbin
authors.forename: J
authors.initials: J
authors.lastname: Lawrence