title: [Investigate of DNA extraction of os cervi].
abstract: OBJECTIVE
  To establish a convenient, practical and high efficient method of DNA extraction of os cervi, and lay the foundation of identification of animal bones.
  
  
  METHODS
  The bones of sika deer, red deer, cattle, dog and pig were used to extract DNA under different decalcification time (24,48,72 h) and decalcification temperature (4,25,37,56,70 degrees C), and extract method.
  
  
  RESULTS
  It proved by experiments that demineralization process promotes the cracking of osteocyte. In a broad of decalcification time and temperature, DNA could be extracted from all bone samples successfully while the quantity varied slightly.
  
  
  CONCLUSIONS
  Samples (about 0.1 g) decalcify with 0. mol x L(-1) EDTA at 4 degrees C for 24 h, then water-bath for 1 h after lysis buffer added, DNA extracted via the method above is of high quality and can be used for PCR.
authors.affiliation: College of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China.
authors.forename: Jingxue
authors.initials: J
authors.lastname: Zhao
authors.forename: Min
authors.initials: M
authors.lastname: Chen
authors.forename: Guanghong
authors.initials: G
authors.lastname: Cui
authors.forename: Shihuan
authors.initials: S
authors.lastname: Tang
authors.forename: Luqi
authors.initials: L
authors.lastname: Huang
authors.forename: Liqun
authors.initials: L
authors.lastname: He
authors.forename: Ruixue
authors.initials: R
authors.lastname: Xia
mesh_terms: D000818:Animals; D018775:Bone Demineralization Technique; D001842:Bone and Bones / Q000737:chemistry* / Q000378:metabolism*; D002417:Cattle; D004247:DNA / Q000302:isolation & purification*; D003670:Deer; D004285:Dogs; D016133:Polymerase Chain Reaction; D013552:Swine; D013696:Temperature; D013997:Time Factors
chemical_list: D004247:DNA