title: Co-immunization with interlukin-18 enhances the protective efficacy of liposomes encapsulated recombinant Cu-Zn superoxide dismutase protein against Brucella abortus.
abstract: Brucellosis is a worldwide zoonotic disease caused by Brucella abortus and a number of closely related species. Brucellosis has severe impact on the health and economic prosperity of the developing countries due to the persistent nature of infection and unavailability of effective control measures. The Cu-Zn superoxide dismuatse (SOD) protein of Brucella have been extensively studied as a major antigen involved in bacterial evading mechanism of host defence. Being a critical pro-inflammatory cytokine interleukin-18 (IL-18) plays key role in induction of immune mediated protection against intracellular pathogens. In the present study, we aimed to investigate the immunogenic potential of fusogenic liposomes (escheriosomes) encapsulated recombinant Cu-Zn SOD (rSOD) protein alone or in combination with recombinant IL-18 (rIL-18). Escheriosomes encapsulated rSOD mediated immune responses were further increased upon co-immunization with rIL-18. Furthermore, immunization with escheriosomes encapsulated rSOD alone or in combination with rIL-18, increased resistance in mice against challenge with B. abortus 544.
authors.affiliation: Division of Biotechnology, Indian Veterinary Research Institute, Izatnagar 243122, India. hsssankarbty@gmail.com
authors.forename: Harisankar
authors.initials: H
authors.lastname: Singha
authors.forename: Amirul Islam
authors.initials: AI
authors.lastname: Mallick
authors.forename: Chandrakanta
authors.initials: C
authors.lastname: Jana
authors.forename: Nishat
authors.initials: N
authors.lastname: Fatima
authors.forename: Mohammad
authors.initials: M
authors.lastname: Owais
authors.forename: Pallab
authors.initials: P
authors.lastname: Chaudhuri
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology; D002004:Brucella Vaccine / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D002003:Brucella abortus / Q000235:genetics / Q000276:immunology*; D002006:Brucellosis / Q000517:prevention & control; D004195:Disease Models, Animal; D005260:Female; D007114:Immunization / Q000379:methods*; D020382:Interleukin-18 / Q000008:administration & dosage*; D008081:Liposomes / Q000008:administration & dosage*; D051379:Mice; D012376:Rodent Diseases / Q000517:prevention & control; D013482:Superoxide Dismutase / Q000235:genetics / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000942:Antigens, Bacterial; D002004:Brucella Vaccine; D020382:Interleukin-18; D008081:Liposomes; D014614:Vaccines, Synthetic; D013482:Superoxide Dismutase