title: Surface plasmon resonance biosensor for determination of tetrodotoxin: prevalidation study.
abstract: A label-free surface plasmon resonance biosensor method was applied to determine tetrodotoxin (TTX) in pufferfish matrixes using an antibody inhibition assay format. A prevalidation study was conducted to demonstrate the assay performance characteristics, such as selectivity, LOD, LOQ, repeatability, reproducibility, and accuracy. Three participating laboratories reported standard curves in buffer and pufferfish matrix. A set of blind samples with TTX spiked into buffer as well as in 10% pufferfish extract were analyzed. Additionally, three blind naturally contaminated samples were analyzed, and the results were compared to those obtained using a reference method (HPLC/electrospray ionization-selected reaction monitoring-MS). The developed method was demonstrated to be capable of detecting TTX in pufferfish matrix standard samples in a broad concentration range (2-9000 ng/mL) with an LOD of 1.5 ng/mL. Between-laboratory recovery values were in the range of 51-190% with a mean of 107%, and 64-180% with a mean of 103% for TTX-spiked samples in buffer and pufferfish matrix, respectively. Between-laboratory recoveries were in the satisfactory range of 101-119% for naturally contaminated samples. This robust, rapid, and noninvasive method may serve as an attractive alternative to established methods for detection of TTX in pufferfish extracts.
authors.affiliation: University of Washington, Department of Chemical Engineering, Box 351750, Seattle, WA 98195, USA.
authors.forename: Hana
authors.initials: H
authors.lastname: Vaisocherová
authors.forename: Allen D
authors.initials: AD
authors.lastname: Taylor
authors.forename: Shaoyi
authors.initials: S
authors.lastname: Jiang
authors.forename: Katerina
authors.initials: K
authors.lastname: Hegnerová
authors.forename: Milan
authors.initials: M
authors.lastname: Vala
authors.forename: Jirí
authors.initials: J
authors.lastname: Homola
authors.forename: Betsy Jean
authors.initials: BJ
authors.lastname: Yakes
authors.forename: Jonathan
authors.initials: J
authors.lastname: Deeds
authors.forename: Stacey
authors.initials: S
authors.lastname: DeGrasse
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance / Q000295:instrumentation* / Q000379:methods; D023721:Tetraodontiformes / Q000378:metabolism; D013779:Tetrodotoxin / Q000737:chemistry* / Q000378:metabolism
chemical_list: D013779:Tetrodotoxin