title: [Role of p38 in cyclic strain induced fibroblast orientation].
abstract: OBJECTIVE
  To assess the role of p38 in fibroblast orientation and to explore the cell signal transduction mechanism of cyclic strain induced cell orientation.
  
  
  METHODS
  Fibroblasts were seeded onto collagen coated flexible membranes. Membranes were then deformed at 10 cycles per minute under 135 mmHg subatmospheric pressure. Orientation angles of cells treated with or without SB203580 were measured with inverted microscope. P38 phosporylation was analyzed with Western blot.
  
  
  RESULTS
  Eighty percent cyclic strain induced cells rotated from 60 degree to 90 degree perpendicular to stretch direction after 4 h strain exposure. P38 phosphorylation reached the peak at 5 min. Fibroblast orientation was inhibited after SB203580 treatment.
  
  
  CONCLUSIONS
  Fibroblast orientation in response to cyclic strain is mediated by p38 phosporylation.
authors.affiliation: Department of Surgery, First Affiliated Hospital of Chongqing Medical University, Chongqing 400013, China. dhuangwen@hotmail.com
authors.forename: Wen
authors.initials: W
authors.lastname: Huang
authors.forename: Jianwu
authors.initials: J
authors.lastname: Zhao
mesh_terms: D002465:Cell Movement; D002478:Cells, Cultured; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology* / Q000201:enzymology*; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology; D008297:Male; D040542:Mechanotransduction, Cellular / Q000502:physiology*; D010766:Phosphorylation; D011725:Pyridines / Q000494:pharmacology; D012867:Skin / Q000166:cytology / Q000201:enzymology; D013314:Stress, Mechanical; D048051:p38 Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D004791:Enzyme Inhibitors; D007093:Imidazoles; D011725:Pyridines; D048051:p38 Mitogen-Activated Protein Kinases; C093642:SB 203580