title: Proteomic analysis of ribosomes: translational control of mRNA populations by glycogen synthase GYS1.
abstract: Ribosomes exist as a heterogenous pool of macromolecular complexes composed of ribosomal RNA molecules, ribosomal proteins, and numerous associated "nonribosomal" proteins. To identify nonribosomal proteins that may modulate ribosome activity, we examined the composition of translationally active and inactive ribosomes using a proteomic multidimensional protein identification technology. Notably, the phosphorylated isoform of glycogen synthase, glycogen synthase 1 (GYS1), was preferentially associated with elongating ribosomes. Depletion of GYS1 affected the translation of a subset of cellular mRNAs, some of which encode proteins that modulate protein biosynthesis. These findings argue that GYS1 abundance, by virtue of its ribosomal association, provides a feedback loop between the energy state of the cells and the translation machinery.
authors.affiliation: Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA.
authors.forename: Gabriele
authors.initials: G
authors.lastname: Fuchs
authors.forename: Camille
authors.initials: C
authors.lastname: Diges
authors.forename: Lori A
authors.initials: LA
authors.lastname: Kohlstaedt
authors.forename: Karen A
authors.initials: KA
authors.lastname: Wehner
authors.forename: Peter
authors.initials: P
authors.lastname: Sarnow
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D015152:Blotting, Northern; D015153:Blotting, Western; D020869:Gene Expression Profiling; D006006:Glycogen Synthase / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D011132:Polyribosomes / Q000378:metabolism*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012269:Ribosomal Proteins / Q000378:metabolism*; D012270:Ribosomes; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D015415:Biomarkers; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D012269:Ribosomal Proteins; D006006:Glycogen Synthase