title: [Relationship between Sp3 and the transcriptional regulation of enamelin gene].
abstract: OBJECTIVE
  To investigate the role of Sp3 in the transcriptional regulation of enamelin gene.
  
  
  METHODS
  By bioinformatic analysis, a putative responsive element for Sp3 was identified. Electrophoretic mobility shift assay was used to examine the interaction between Sp3 and enamelin. 5'-flanking regulatory region of enamelin was cloned and ligated into pGL3-basic luciferase vector. Sp3 and the Enam-luc were cotransfected into mouse ameloblast-like cell line, and the activity of luciferase was examined.
  
  
  RESULTS
  The results showed that Sp3 could not directly bind to the enamelin regulation region and activate enamelin transcription.
  
  
  CONCLUSIONS
  Sp3 might not be involved in transcriptional regulation of enamelin gene via an indirect interaction.
authors.affiliation: Department of Cariology and Endodontology, Peking University School, Beijing 100081, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Lü
authors.forename: Hua
authors.initials: H
authors.lastname: Tian
authors.forename: Xue-jun
authors.initials: XJ
authors.lastname: Gao
mesh_terms: D024506:5' Flanking Region / Q000235:genetics; D000565:Ameloblasts / Q000166:cytology; D000818:Animals; D002460:Cell Line; D003746:Dental Enamel Proteins / Q000235:genetics* / Q000378:metabolism*; D024202:Electrophoretic Mobility Shift Assay; D005260:Female; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D008156:Luciferases; D008297:Male; D051379:Mice; D011401:Promoter Regions, Genetic; D051381:Rats; D017207:Rats, Sprague-Dawley; D050436:Regulatory Elements, Transcriptional; D051705:Sp3 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D014162:Transfection
chemical_list: D003746:Dental Enamel Proteins; C038456:tuftelin; D051705:Sp3 Transcription Factor; D008156:Luciferases