title: Preparation, characterization and transfection efficacy of chitosan nanoparticles containing the intestinal trefoil factor gene.
abstract: Intestinal trefoil factor (ITF) is a novel polypeptide with potential pharmacological value for the prevention and healing of tissue injury; however, poor production capacity limits its clinical application. Chitosan, as a non-viral vehicle, has been successfully used in gene delivery for its intrinsic characteristics. In this context, we prepared chitosan nanoparticles enwrapping ITF cDNA and investigated its size, zeta potential, stability, release profiles, loading efficiency and loading capacity. Gene transfer capability was assessed in HEK293 cells. The data revealed that the chitosan/DNA nanoparticles were successfully prepared with sizes less than 500 nm and positive zeta potentials. The nanoparticles could protect DNA from nuclease degradation, and release profiles of DNA were dependent on N/P ratios. In addition, transfection efficiency of chitosan/DNA nanoparticles was equivalent to Lipofectamine (TM). Collectively, the results suggest that chitosan/DNA nanoparticles could be a promising method for ITF gene therapy.
authors.affiliation: State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Burn Research, Southwest Hospital, The Third Military Medical University, Chongqing 400038, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Sun
authors.forename: Shuai
authors.initials: S
authors.lastname: Zhang
authors.forename: Xi
authors.initials: X
authors.lastname: Peng
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Gong
authors.forename: Xiaolu
authors.initials: X
authors.lastname: Li
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Yuan
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.forename: Dawei
authors.initials: D
authors.lastname: Zhang
authors.forename: Yizhi
authors.initials: Y
authors.lastname: Peng
mesh_terms: D015153:Blotting, Western; D048271:Chitosan / Q000737:chemistry*; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D024202:Electrophoretic Mobility Shift Assay; D005434:Flow Cytometry; D015316:Genetic Therapy / Q000379:methods; D057809:HEK293 Cells; D006801:Humans; D007211:Indoles; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry*; D010455:Peptides / Q000235:genetics*; D014162:Transfection / Q000379:methods*; D000071164:Trefoil Factor-2
chemical_list: D018076:DNA, Complementary; D007211:Indoles; D010455:Peptides; D000071164:Trefoil Factor-2; C007293:DAPI; D048271:Chitosan