title: Helicobacter pylori exhibits a fur-dependent acid tolerance response.
abstract: BACKGROUND
  Helicobacter pylori colonizes the acid environment of the gastric mucosa. Like other enteric bacterial pathogens, including Salmonella enterica, which must survive a brief exposure to that environment, H. pylori displays a rapid response to subtle changes in pH, which confers an increased ability to survive at more extreme acidic pH. This two-step acid tolerance response (ATR) requires de novo protein synthesis and is dependent on the function of the global regulatory protein Fur.
  
  
  OBJECTIVE
  We have explored the physiological bases of the ATR in H. pylori.
  
  
  METHODS
  Proteomic analysis of phenotypes of H. pylori and fur mutant strains show that subtle pH changes elicit significant changes in the pattern of proteins synthesized.
  
  
  RESULTS
  A loss-of-function mutation in the fur gene, obtained by insertion of an antibiotic resistance cassette, indicated that Fur regulates the expression of a fraction of H. pylori proteins.
  
  
  CONCLUSIONS
  A subset of proteins is involved in the ATR and confer a negative ATR phenotype.
authors.affiliation: Programa de Biología Celular y Molecular, ICBM, Facultad de Medicina, Universidad de Chile, Casilla 70086, Santiago-7, Chile.
authors.forename: Manuel
authors.initials: M
authors.lastname: Valenzuela
authors.forename: Juan P
authors.initials: JP
authors.lastname: Albar
authors.forename: Alberto
authors.initials: A
authors.lastname: Paradela
authors.forename: Héctor
authors.initials: H
authors.lastname: Toledo
mesh_terms: D000143:Acids / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D016480:Helicobacter pylori / Q000235:genetics / Q000378:metabolism*; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D000143:Acids; D001426:Bacterial Proteins; D012097:Repressor Proteins