title: Polyhemoglobin-superoxide dismutase-catalase-carbonic anhydrase: a novel biotechnology-based blood substitute that transports both oxygen and carbon dioxide and also acts as an antioxidant.
abstract: Polyhemoglobin-superoxide dismutase-catalase-carbonic anhydrase (PolyHb-SOD-CAT-CA) is a therapeutic antioxidant that also transports both oxygen and carbon dioxide. This is formed by crosslinking Hb with SOD, CAT, and CA using glutaraldehyde. Crosslinking stroma free Hb from red blood cell (rbc) reduces CA activity to 55%. Addition of more CA resulted in a preparation with the same CA activity as RBC. PolyHb in the complex acts as a buffer to prevent large pH changes as carbon dioxide is converted to carbonic acid. We then prepare and optimize a novel PolyHb-SOD-CAT-CA, a therapeutic antioxidant that also transports both oxygen and carbon dioxide.
authors.affiliation: Artificial Cells and Organs Research Centre, Departments of Physiology, Medicine and Biomedical Engineering, Faculty of Medicine, McGill University, Montreal, Quebec, Canada.
authors.forename: Yuzhu
authors.initials: Y
authors.lastname: Bian
authors.forename: Zhixia
authors.initials: Z
authors.lastname: Rong
authors.forename: Thomas Ming Swi
authors.initials: TM
authors.lastname: Chang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism* / Q000627:therapeutic use; D001709:Biotechnology; D001802:Blood Substitutes / Q000737:chemistry / Q000378:metabolism* / Q000627:therapeutic use; D002245:Carbon Dioxide / Q000378:metabolism; D002256:Carbonic Anhydrases / Q000737:chemistry / Q000378:metabolism* / Q000627:therapeutic use; D002374:Catalase / Q000737:chemistry / Q000378:metabolism* / Q000627:therapeutic use; D002417:Cattle; D005976:Glutaral / Q000378:metabolism; D006454:Hemoglobins / Q000737:chemistry / Q000378:metabolism* / Q000627:therapeutic use; D006801:Humans; D009097:Multienzyme Complexes / Q000737:chemistry / Q000378:metabolism* / Q000627:therapeutic use; D036103:Nanotechnology; D018384:Oxidative Stress / Q000187:drug effects; D010100:Oxygen / Q000378:metabolism; D058105:Polymerization; D015427:Reperfusion Injury / Q000378:metabolism / Q000628:therapy*; D013482:Superoxide Dismutase / Q000737:chemistry / Q000378:metabolism* / Q000627:therapeutic use
chemical_list: D000975:Antioxidants; D001802:Blood Substitutes; D006454:Hemoglobins; D009097:Multienzyme Complexes; D002245:Carbon Dioxide; D002374:Catalase; D013482:Superoxide Dismutase; C086134:polyhemoglobin-superoxide dismutase-catalase; C582341:polyhemoglobin-superoxide dismutase-catalase-carbonic anhydrase; D002256:Carbonic Anhydrases; D010100:Oxygen; D005976:Glutaral