title: The identification and characterization of zebrafish hematopoietic stem cells.
abstract: HSCs are defined by their ability to self-renew and maintain hematopoiesis throughout the lifespan of an organism. The optical clarity of their embryos and the ease of genetic manipulation make the zebrafish (Danio rerio) an excellent model for studying hematopoiesis. Using flow cytometry, we identified 2 populations of CD41-GFP(+) cells (GFP(hi) and GFP(lo)) in the whole kidney marrow of Tg(CD41:GFP) zebrafish. Past studies in humans and mice have shown that CD41 is transiently expressed in the earliest hematopoietic progenitors and is then silenced, reappearing in the platelet/thrombocyte lineage. We have transplanted flow-sorted GFP(hi) and GFP(lo) cells into irradiated adult zebrafish and assessed long-term hematopoietic engraftment. Transplantation of GFP(hi) cells did not reconstitute hematopoiesis. In contrast, we observed multilineage hematopoiesis up to 68 weeks after primary and secondary transplantation of GFP(lo) cells. We detected the CD41-GFP transgene in all major hematopoietic lineages and CD41-GFP(+) cells in histologic sections of kidneys from transplant recipients. These studies show that CD41-GFP(lo) cells fulfill generally accepted criteria for HSCs. The identification of fluorescent zebrafish HSCs, coupled with our ability to transplant them into irradiated adult recipients, provide a valuable new tool to track HSC homing, proliferation, and differentiation into hematopoietic cells.
authors.affiliation: Hematology Division, Brigham and Women's Hospital, Boston, MA 02115, USA.
authors.forename: Dongdong
authors.initials: D
authors.lastname: Ma
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Hui-feng
authors.initials: HF
authors.lastname: Lin
authors.forename: Joseph
authors.initials: J
authors.lastname: Italiano
authors.forename: Robert I
authors.initials: RI
authors.lastname: Handin
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001792:Blood Platelets / Q000166:cytology / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology; D004625:Embryo, Nonmammalian; D005434:Flow Cytometry; D005720:Gamma Rays; D006085:Graft Survival; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006410:Hematopoiesis / Q000235:genetics / Q000502:physiology; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000528:radiation effects; D040201:Platelet Membrane Glycoprotein IIb / Q000235:genetics; D015027:Zebrafish / Q000196:embryology / Q000235:genetics / Q000502:physiology
chemical_list: D040201:Platelet Membrane Glycoprotein IIb; D049452:Green Fluorescent Proteins