title: Advances in the production of membrane proteins in Pichia pastoris.
abstract: Membrane proteins play key roles in diverse cellular functions and have become the target for a large number of pharmacological drugs. Despite representing about 20-30% of cellular proteins, their characterization is long overdue since they are difficult to handle, to purify from their natural source or to obtain as recombinant proteins. Pichia pastoris is a methylotrophic yeast species increasingly used as a host for heterologous protein expression for both research and industrial purposes. Over the past few years many efforts have allowed important advances in the development of this expression system for the expression and production of membrane proteins. The most recent achievements in improving yield and proper folding of integral membrane proteins are summarized in this review.
authors.affiliation: Sección Bioquímica-Biología Molecular, Facultad de Ciencias, Iguá 4225, Montevideo, Uruguay. anaramon@fcien.edu.uy
authors.forename: Ana
authors.initials: A
authors.lastname: Ramón
authors.forename: Mónica
authors.initials: M
authors.lastname: Marín
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods; D003062:Codon; D015870:Gene Expression; D005822:Genetic Vectors; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics; D010843:Pichia / Q000235:genetics / Q000378:metabolism; D015202:Protein Engineering; D017510:Protein Folding; D011499:Protein Processing, Post-Translational / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics
chemical_list: D003062:Codon; D008565:Membrane Proteins; D011994:Recombinant Proteins