title: Antioxidant and antigenotoxic activities in Acacia salicina extracts and its protective role against DNA strand scission induced by hydroxyl radical.
abstract: The antioxidant potency of Acacia salicina extracts was investigated. Total antioxidant capacity was determined using an ABTS(+) assay. Superoxide radical scavenging was measured using riboflavin-light-nitro blue tetrazolium (NBT) assay. In addition, the content of phenols, total flavonoids and sterols were measured in the tested extracts. The petroleum ether exhibited a potent scavenging activity toward ABTS radical cations. Whereas, chloroform extract showed the highest activity against superoxides radicals and was also able to protect pKS plasmid DNA against hydroxyl radicals induced DNA damages. The antimutagenicity of these extracts was assayed using the Ames assay against Salmonella typhimurium TA98 and S. typhimurium TA 1535 tester strains at different concentrations. These extracts decreased significantly the mutagenecity induced by sodium azide (SA) and 4-nitro-o-phenylenediamine (NOP). The antioxidant and antimutagenecity activities exhibited by A. salicina depended on the chemical composition of the tested extracts.
authors.affiliation: Unité de Pharmacognosie/Biologie moléculaire 99/UR/07-03, Faculté de Pharmacie/Médecine Dentaire de Monastir, Tunisia. leila.chekir@laposte.net
authors.forename: Ines Bouhlel
authors.initials: IB
authors.lastname: Chatti
authors.forename: Jihed
authors.initials: J
authors.lastname: Boubaker
authors.forename: Ines
authors.initials: I
authors.lastname: Skandrani
authors.forename: Wissem
authors.initials: W
authors.lastname: Bhouri
authors.forename: Kamel
authors.initials: K
authors.lastname: Ghedira
authors.forename: Leila
authors.initials: L
authors.lastname: Chekir Ghedira
mesh_terms: D000045:Acacia / Q000737:chemistry*; D016587:Antimutagenic Agents / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology*; D004249:DNA Damage / Q000187:drug effects*; D005419:Flavonoids / Q000032:analysis / Q000494:pharmacology; D017665:Hydroxyl Radical / Q000633:toxicity*; D010636:Phenols / Q000032:analysis / Q000494:pharmacology; D010655:Phenylenediamines / Q000633:toxicity; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D010957:Plasmids / Q000187:drug effects; D012486:Salmonella typhimurium / Q000187:drug effects; D019810:Sodium Azide / Q000633:toxicity; D013261:Sterols / Q000032:analysis / Q000494:pharmacology
chemical_list: D016587:Antimutagenic Agents; D000975:Antioxidants; D005419:Flavonoids; D010636:Phenols; D010655:Phenylenediamines; D010936:Plant Extracts; D013261:Sterols; D017665:Hydroxyl Radical; C012277:1,2-diamino-4-nitrobenzene; D019810:Sodium Azide