title: PERMANENT GENETIC RESOURCES: Isolation and characterization of microsatellite loci in the North Island brown kiwi, Apteryx mantelli.
abstract: We report the isolation and characterization of eight polymorphic and five monomorphic microsatellites in North Island brown kiwi (NIBK, Apteryx mantelli), using two polymerase chain reaction (PCR) techniques employing either short-tandem repeat primers (STR method) or random PCR-based isolation of microsatellite arrays (PIMA method). Microsatellite polymorphism was subsequently determined using 65 individuals. There were two to seven alleles for each polymorphic locus with heterozygozity ranging between 0.04 and 0.86. These primers will be used in future studies to determine the level of extra-pair copulation, dispersal patterns, and genetic diversity within and between wild populations of NIBK.
authors.affiliation: Conservation and Research for Endangered Species, Zoological Society of San Diego, San Diego, CA, USA, Department of Biological Sciences, University of Waikato, Hamilton 3240, New Zealand, School of Biological Sciences, University of Auckland, Auckland, New Zealand, Poultry Microbiological Safety Research Unit, Russell Research Center, ARS, USDA, Athens, GA, USA.
authors.forename: Thomas
authors.initials: T
authors.lastname: Jensen
authors.forename: Karen J
authors.initials: KJ
authors.lastname: Nutt
authors.forename: Bruce S
authors.initials: BS
authors.lastname: Seal
authors.forename: Luiza B
authors.initials: LB
authors.lastname: Fernandes
authors.forename: Barbara
authors.initials: B
authors.lastname: Durrant