title: Qualitative and quantitative analyses of Epimedium wushanense by high-performance liquid chromatography coupled with diode array detection and electrospray ionization tandem mass spectrometry.
abstract: A new HPLC-DAD-ESI-MS(n) method was developed for rapid separation, characterization and quantitation of flavonoids in Epimedium wushanense, a popular Chinese herbal medicine. For qualitative identification, a total of 37 compounds were characterized from the underground and aerial parts of E. wushanense. Among them, 28 compounds were prenylated flavonoids, and 23 were confirmed by comparing with reference standards. For quantitative analysis, 12 major flavonoids including kaempferol glycosides, desmethylicaritin glycosides, and icaritin glycosides were simultaneously determined by HPLC/UV. Samples were separated on a Waters Symmetry C(18) column at 35 °C eluted with a gradient three-component mobile phase of acetonitrile, methanol, and water containing 0.03% v/v formic acid. All the flavonoids showed good linearity (r(2) ≥0.9997). The recoveries varied from 92.6 to 106.1% at three concentration levels. This method was applied to the determination of 20 samples of different geographical sources, harvesting time, and plant parts. Contents of the predominant flavonoid, epimedin C, ranged from 1.4 to 5.1% in aerial parts and 1.0 to 2.8% in underground parts. The methods established in this paper were simple and reliable and could be used for the quality control of E. wushanense.
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, PR China.
authors.forename: Hui-fang
authors.initials: HF
authors.lastname: Li
authors.forename: Xiang-yu
authors.initials: XY
authors.lastname: Guan
authors.forename: Min
authors.initials: M
authors.lastname: Ye
authors.forename: Cheng
authors.initials: C
authors.lastname: Xiang
authors.forename: Chang-hu
authors.initials: CH
authors.lastname: Lin
authors.forename: Chao
authors.initials: C
authors.lastname: Sun
authors.forename: De-an
authors.initials: DA
authors.lastname: Guo
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D034181:Epimedium / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D011786:Quality Control; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D010936:Plant Extracts