title: 16S rRNA gene microarray analysis of microbial communities in ethanol-stimulated subsurface sediment.
abstract: A high-density 16S rRNA gene microarray was used to analyze microbial communities in a slurry of ethanol-amended, uranium-contaminated subsurface sediment. Of specific interest was the extent to which the microarray could detect temporal patterns in the relative abundance of major metabolic groups (nitrate-reducing, metal-reducing, sulfate-reducing, and methanogenic taxa) that were stimulated by ethanol addition. The results show that the microarray, when used in conjunction with geochemical data and knowledge of the physiological properties of relevant taxa, provided accurate assessment of the response of key functional groups to biostimulation.
authors.affiliation: Department of Geoscience, University of Wisconsin, Madison, 1215 W. Dayton St., WI 53706, USA.
authors.forename: Santosh R
authors.initials: SR
authors.lastname: Mohanty
authors.forename: Bharati
authors.initials: B
authors.lastname: Kollah
authors.forename: Eoin L
authors.initials: EL
authors.lastname: Brodie
authors.forename: Terry C
authors.initials: TC
authors.lastname: Hazen
authors.forename: Eric E
authors.initials: EE
authors.lastname: Roden
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D015373:Bacterial Typing Techniques / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics*; D000431:Ethanol / Q000378:metabolism*; D019015:Geologic Sediments / Q000737:chemistry / Q000382:microbiology*; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D014501:Uranium / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D000431:Ethanol; D014501:Uranium