title: [Construction of the eukaryotic expression vector encoding multi-epitope fusion protein of human survivin and its expression in dendritic cells].
abstract: OBJECTIVE
  To construct a eukaryotic expression vector encoding the multi-epitope fusion protein of human survivin, and express it in human dendritic cells.
  
  
  METHODS
  Recombinant cDNA sequence encoding four HLA-A2-restricted CD8+ CTL epitopes and a CD4+ Th epitope was synthesized and cloned into pBluescript II SK (+) vector. After confirmed by sequencing, the cDNA fragment was inserted to eukaryotic expression vector pIRESneo3.0 to generate the recombinant plasmid pPIRESneo3.0-survivin (4)/Th. The pPIRESneo3.0-survivin (4)/Th was then transfected into human dendritic cells and the transfectants were selected for stable expression.
  
  
  RESULTS
  The eukaryotic expression vector encoding the multi-epitope fusion protein of survivin was constructed, and successfully transfected into human dendritic cells.
  
  
  CONCLUSIONS
  The eukaryotic expression vector encoding the multi-epitope fusion protein of survivin has been constructed successfully, and stably expressed in human dendritic cells, which provides clues for further research on multi-epitope cancer vaccine.
authors.affiliation: Department of General Surgery, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China. wangxy5829003@126.com
authors.forename: Xiao-yuan
authors.initials: XY
authors.lastname: Wang
authors.forename: Wen-zhe
authors.initials: WZ
authors.lastname: Song
authors.forename: Xiang-nong
authors.initials: XN
authors.lastname: Li
authors.forename: Meng
authors.initials: M
authors.lastname: Guo
authors.forename: Xiao
authors.initials: X
authors.lastname: Liang
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D003713:Dendritic Cells / Q000378:metabolism / Q000502:physiology*; D000939:Epitopes / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D005822:Genetic Vectors / Q000737:chemistry / Q000235:genetics; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D000077022:Survivin
chemical_list: C107489:BIRC5 protein, human; D000939:Epitopes; D051034:Inhibitor of Apoptosis Proteins; D011993:Recombinant Fusion Proteins; D000077022:Survivin