title: Detection of head-to-tail DNA sequences of human bocavirus in clinical samples.
abstract: Parvoviruses are single stranded DNA viruses that replicate in a so called "rolling-hairpin" mechanism, a variant of the rolling circle replication known for bacteriophages like φX174. The replication intermediates of parvoviruses thus are concatemers of head-to-head or tail-to-tail structure. Surprisingly, in case of the novel human bocavirus, neither head-to-head nor tail-to-tail DNA sequences were detected in clinical isolates; in contrast head-to-tail DNA sequences were identified by PCR and sequencing. Thereby, the head-to-tail sequences were linked by a novel sequence of 54 bp of which 20 bp also occur as conserved structures of the palindromic ends of parvovirus MVC which in turn is a close relative to human bocavirus.
authors.affiliation: Institut für Pathologie, Kliniken der Stadt Köln gGmbH, Klinikum der Privaten Universität Witten-Herdecke, Köln, Cologne, Germany.
authors.forename: Jessica
authors.initials: J
authors.lastname: Lüsebrink
authors.forename: Verena
authors.initials: V
authors.lastname: Schildgen
authors.forename: Ramona Liza
authors.initials: RL
authors.lastname: Tillmann
authors.forename: Felix
authors.initials: F
authors.lastname: Wittleben
authors.forename: Anne
authors.initials: A
authors.lastname: Böhmer
authors.forename: Andreas
authors.initials: A
authors.lastname: Müller
authors.forename: Oliver
authors.initials: O
authors.lastname: Schildgen
mesh_terms: D004247:DNA / Q000737:chemistry*; D056661:Human bocavirus / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D004247:DNA