title: Investigation of the growth of focal adhesions using protein nanoarrays fabricated by nanocontact printing using size tunable polymeric nanopillars.
abstract: Here we describe a simple approach to create various sizes of protein nanoarrays for the investigation of cell adhesion. Using a combination of nanosphere lithography, oxygen plasma treatment, deep etching and nanomolding processes, well-ordered polymeric nanopillar arrays have been fabricated with diameters in the range of 50-600 nm. These nanopillar arrays were used as stamps for nanocontact printing to create fibronectin nanoarrays, which were used to study the size dependent formation of focal adhesion. It was found that cells can adhere and spread on fibronectin nanoarrays with a fibronectin pattern as small as 50 nm. It was also found that the average size of focal adhesion decreased as the size of the fibronectin pattern was reduced.
authors.affiliation: Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan.
authors.forename: Chiung Wen
authors.initials: CW
authors.lastname: Kuo
authors.forename: Fan-Ching
authors.initials: FC
authors.lastname: Chien
authors.forename: Jau-Ye
authors.initials: JY
authors.lastname: Shiu
authors.forename: Shih-Min
authors.initials: SM
authors.lastname: Tsai
authors.forename: Di-Yan
authors.initials: DY
authors.lastname: Chueh
authors.forename: Yu-Sheng
authors.initials: YS
authors.lastname: Hsiao
authors.forename: Zong-Han
authors.initials: ZH
authors.lastname: Yang
authors.forename: Peilin
authors.initials: P
authors.lastname: Chen
mesh_terms: D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D005353:Fibronectins / Q000737:chemistry*; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D022001:Focal Adhesions / Q000378:metabolism*; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods*; D010316:Particle Size; D011108:Polymers / Q000737:chemistry*; D040081:Protein Array Analysis / Q000379:methods*; D012825:Silicon / Q000737:chemistry
chemical_list: D005353:Fibronectins; D011108:Polymers; D016650:Fluorescein-5-isothiocyanate; D012825:Silicon