title: Improved electrochemical immunosensor for myeloperoxidase in human serum based on nanogold/cerium dioxide-BMIMPF6/L-Cysteine composite film.
abstract: An electrochemical immunosensing assay for myeloperoxidase (MPO) determination in human serum has been developed. Firstly, L-Cysteine was initially electropolymerized on an Au electrode to form L-Cysteine film. After that cerium dioxide (CeO2) dispersed in 1-butyl-3-methylimidazolium hexafluorophosphate (BMIMPF6) were immobilized on the L-Cysteine film. Then the negatively charged nanogold particles were adsorbed onto the membrane via the positive charge of CeO2, which aimed at assembling more antibody of MPO (anti-MPO). The resulting immunosensor showed a high sensitivity, broad linear response to the MPO concentration comprised between 10 ng/mL and 400 ng/mL with a detection limit of 0.06 ng/mL. Moreover, the surface morphology of the electrode was studied by means of a scanning electron microscope and the electrochemical properties of the fabricated immunosensor were further characterized by cyclic voltammetry. Also, factors influencing the performance of the resulting immunosensors were studied in detail.
authors.affiliation: Key Laboratory of Medical Diagnostics of Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, PR China.
authors.forename: Lingsong
authors.initials: L
authors.lastname: Lu
authors.forename: Bei
authors.initials: B
authors.lastname: Liu
authors.forename: Shenfeng
authors.initials: S
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Guoming
authors.initials: G
authors.lastname: Xie
mesh_terms: D054058:Acute Coronary Syndrome / Q000097:blood; D000906:Antibodies / Q000276:immunology; D015415:Biomarkers / Q000097:blood*; D015374:Biosensing Techniques / Q000379:methods*; D002563:Cerium / Q000737:chemistry; D003545:Cysteine / Q000737:chemistry; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D017743:Equipment Reuse; D006046:Gold / Q000737:chemistry; D006801:Humans; D007093:Imidazoles / Q000737:chemistry; D018796:Immunoconjugates / Q000737:chemistry*; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D009195:Peroxidase / Q000097:blood* / Q000276:immunology; D011199:Potentiometry; D012680:Sensitivity and Specificity
chemical_list: C412621:1-butyl-3-methylimidazolium hexafluorophosphate; D000906:Antibodies; D015415:Biomarkers; D007093:Imidazoles; D018796:Immunoconjugates; D002563:Cerium; C030583:ceric oxide; D006046:Gold; D009195:Peroxidase; D003545:Cysteine