title: miR-185 and miR-133b deregulation is associated with overall survival and metastasis in colorectal cancer.
abstract: Colorectal cancer (CRC) is one of the most common and deadly forms of cancer. Despite improved treatment modalities, post-operative recurrence and metastasis remain the major problems for extending patient survival after surgery. This highlights the need to search for biomarkers for prognostication and treatment stratification of colorectal cancer patients. In this study, we applied the SYBR-green quantitative PCR-based array approach to screen for differentially expressed miRNAs between patients with short (<50 months, range 10-33 months) and long survival (≥ 50 months, range 50-152 months). The selected candidate prognostic miRNAs were validated in a cohort of 50 CRC patients by TaqMan quantitative PCR. We found that high expression of miR-185 and low expression of miR-133b were correlated with poor survival (p=0.001 and 0.028, respectively) and metastasis (p=0.007 and 0.036, respectively) in colorectal cancer. Our findings suggest the potential prognostic values of these miRNAs for predicting clinical outcome after surgery.
authors.affiliation: Department of Molecular Medicine and Surgery, CMM L8:01, Karolinska University Hospital-Solna, SE-17176 Stockholm, Sweden. pinar.akcakaya@ki.se
authors.forename: Pinar
authors.initials: P
authors.lastname: Akçakaya
authors.forename: Susanne
authors.initials: S
authors.lastname: Ekelund
authors.forename: Iryna
authors.initials: I
authors.lastname: Kolosenko
authors.forename: Stefano
authors.initials: S
authors.lastname: Caramuta
authors.forename: Deniz M
authors.initials: DM
authors.lastname: Ozata
authors.forename: Hong
authors.initials: H
authors.lastname: Xie
authors.forename: Ulrik
authors.initials: U
authors.lastname: Lindforss
authors.forename: Hans
authors.initials: H
authors.lastname: Olivecrona
authors.forename: Weng-Onn
authors.initials: WO
authors.lastname: Lui
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016000:Cluster Analysis; D015179:Colorectal Neoplasms / Q000401:mortality / Q000503:physiopathology*; D015536:Down-Regulation; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009362:Neoplasm Metastasis; D011379:Prognosis
chemical_list: D035683:MicroRNAs