title: TGFBI expression reduces in vitro and in vivo metastatic potential of lung and breast tumor cells.
abstract: Controversy has arisen as to the role of transforming growth factor-β-induced protein (TGFBI) in the regulation of tumor metastasis. Using lung and breast cancer cell lines (H522 and MCF-7, respectively), we established that TGFBI induced cell adhesion to extracellular matrix proteins by activating adhesion-associated signaling and subsequent structure reformation, ultimately leading to cells less motile; whereas TGFBI reduced abilities of colony formation in soft agar, penetration through matrix gel, and activation of matrix metalloproteinases 2 and 9. Furthermore, injection of TGFBI-expressing cells into immuno-deficient mice resulted in a significant reduction in tumor metastasis in vivo. Taken together, these data suggest that TGFBI moderates the metastatic potential of cancer cells.
authors.affiliation: Center for Radiological Research, College of Physicians & Surgeons, Columbia University, New York, NY, USA. gw2154@columbia.edu
authors.forename: Gengyun
authors.initials: G
authors.lastname: Wen
authors.forename: Michael A
authors.initials: MA
authors.lastname: Partridge
authors.forename: Bingyan
authors.initials: B
authors.lastname: Li
authors.forename: Mei
authors.initials: M
authors.lastname: Hong
authors.forename: Wupeng
authors.initials: W
authors.lastname: Liao
authors.forename: Simon K
authors.initials: SK
authors.lastname: Cheng
authors.forename: Yongliang
authors.initials: Y
authors.lastname: Zhao
authors.forename: Gloria M
authors.initials: GM
authors.lastname: Calaf
authors.forename: Tian
authors.initials: T
authors.lastname: Liu
authors.forename: Jun
authors.initials: J
authors.lastname: Zhou
authors.forename: Zengli
authors.initials: Z
authors.lastname: Zhang
authors.forename: Tom K
authors.initials: TK
authors.lastname: Hei
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D002448:Cell Adhesion / Q000502:physiology; D045744:Cell Line, Tumor; D003599:Cytoskeleton / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D051379:Mice; D009362:Neoplasm Metastasis; D015398:Signal Transduction; D014162:Transfection; D053773:Transforming Growth Factor beta1 / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism
chemical_list: D053773:Transforming Growth Factor beta1; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9