title: Anaphase DNA bridges induced by lack of RecQ5 in Drosophila syncytial embryos.
abstract: Drosophila melanogaster RecQ5, a member of the RecQ family, is expressed in early embryos. The loss of maternally-derived RecQ5 leads to spontaneous mitotic defects in syncytial embryos. We demonstrate that the mitotic defects are derived from anaphase DNA bridges. Pairs of daughter nuclei that had been linked by the bridges concurrently exited from the cycle and were eliminated by Chk2-dependent centrosome inactivation. These results suggest that the lack of RecQ5 leads to spontaneous double-stranded DNA breaks (DSBs). RecQ5 may function in the resolution of anaphase DNA bridges during mitosis or in DSB repair during interphase in syncytial Drosophila embryos.
authors.affiliation: Division of Biochemistry, Faculty of Pharmaceutical Science, Setsunan University, Hirakata, Osaka, Japan.
authors.forename: Haruna
authors.initials: H
authors.lastname: Sakurai
authors.forename: Misa
authors.initials: M
authors.lastname: Okado
authors.forename: Fumiaki
authors.initials: F
authors.lastname: Ito
authors.forename: Katsumi
authors.initials: K
authors.lastname: Kawasaki
mesh_terms: D000705:Anaphase / Q000502:physiology*; D000818:Animals; D064447:Checkpoint Kinase 2; D004247:DNA / Q000648:ultrastructure*; D053903:DNA Breaks, Double-Stranded; D004265:DNA Helicases; D029721:Drosophila Proteins / Q000172:deficiency*; D004331:Drosophila melanogaster / Q000166:cytology / Q000196:embryology* / Q000378:metabolism*; D004625:Embryo, Nonmammalian / Q000166:cytology*; D015726:Giant Cells; D008938:Mitosis; D017346:Protein Serine-Threonine Kinases; D053484:RecQ Helicases / Q000172:deficiency*
chemical_list: D029721:Drosophila Proteins; D004247:DNA; D064447:Checkpoint Kinase 2; D017346:Protein Serine-Threonine Kinases; D004265:DNA Helicases; D053484:RecQ Helicases; C407462:RecQ5 protein, Drosophila