title: Ability to use the diazo dye, C.I. Acid Black 1 as a nitrogen source by the marine cyanobacterium Oscillatoria curviceps BDU92191.
abstract: Ten different strains of marine cyanobacteria were tested for their ability to decolourise and degrade a recalcitrant diazo dye, C.I. Acid Black 1. Of them, Oscillatoria curvicepsBDU92191 was able to grow up to a tested concentration of 500 mG L(-1). The organism degraded 84% of the dye at 100 mG L(-1) in 8 days in a medium free of combined nitrogen. The dye degrading ability is attributed to the activities of the enzymes: laccase, polyphenol oxidase and azoreductase. The absence of the doublet amine peak in addition to the overall reduction of absorption in the IR spectra confirmed the mineralisation of the tested azo dye. The nitrogen assimilating enzyme studies along with nitrogenase assay strongly suggested the ability of the non-heterocystous, filamentous marine cyanobacterium, O. curvicepsBDU92191 to use C.I. Acid Black 1 as a nitrogen source in an oligotrophic environment.
authors.affiliation: National Facility for Marine Cyanobacteria (Sponsored by DBT, Government of India), Department of Marine Biotechnology, School of Marine Sciences, Bharathidasan University, Tiruchirappalli, Tamil Nadu 620 024, India.
authors.forename: Balakrishnan
authors.initials: B
authors.lastname: Priya
authors.forename: Lakshmanan
authors.initials: L
authors.lastname: Uma
authors.forename: Abdul Khaleel
authors.initials: AK
authors.lastname: Ahamed
authors.forename: Gopalakrishnan
authors.initials: G
authors.lastname: Subramanian
authors.forename: Dharmar
authors.initials: D
authors.lastname: Prabaharan
mesh_terms: D000580:Amido Black; D001673:Biodegradation, Environmental / Q000187:drug effects; D004156:Catechol Oxidase / Q000378:metabolism; D004396:Coloring Agents / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D042845:Laccase / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism / Q000494:pharmacology*; D054300:Oscillatoria / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D004396:Coloring Agents; D004156:Catechol Oxidase; D042845:Laccase; D009584:Nitrogen; D000580:Amido Black