title: Determination of fumonisins B₁ and B₂ in maize food products by a new analytical method based on high-performance liquid chromatography and fluorimetric detection with post-column derivatization.
abstract: A sensitive and selective analytical method for the quantitative determination of fumonisins B(1) (FB(1)) and B(2) (FB(2)) in maize-based foods for direct human consumption is described. The method, based on high-performance liquid chromatography and fluorescence detection, presents a rapid and automated online post-column derivatization, performed with o-phthalaldehyde and N,N-dimethyl-2-mercaptoethylamine (Thiofluor™). A complete separation of fumonisins is achieved in less than 13 min by using a C18 column and a gradient elution. Fumonisins are extracted from the sample with a mixture of water, acetonitrile, and methanol. The filtered extract is purified by immunoaffinity column and FB(1) and FB(2) are eluted with methanol. The method has been successfully validated, and performances comply with -criteria of the Regulation EC No 401/2006.
authors.affiliation: Dipartimento di Scienze Agro-Ambientali, Chimica e Difesa Vegetale and BIOAGROMED, Università degli Studi di Foggia, Foggia, Italy.
authors.forename: Marilena
authors.initials: M
authors.lastname: Muscarella
authors.forename: Sonia Lo
authors.initials: SL
authors.lastname: Magro
authors.forename: Donatella
authors.initials: D
authors.lastname: Nardiello
authors.forename: Carmen
authors.initials: C
authors.lastname: Palermo
authors.forename: Diego
authors.initials: D
authors.lastname: Centonze
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005470:Fluorometry / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D037341:Fumonisins / Q000032:analysis*; D003313:Zea mays / Q000737:chemistry* / Q000382:microbiology; D009764:o-Phthalaldehyde / Q000032:analysis
chemical_list: D037341:Fumonisins; C056934:fumonisin B2; C056933:fumonisin B1; D009764:o-Phthalaldehyde