title: [Effects of electroacupuncture on expression of tyrosine hydroxylase and c-fos in hippocampal CA 1 area in ketamine-addiction rats].
abstract: OBJECTIVE
  To explore the morphological basis of electroacupuncture (EA) in relieving addiction of ketamine.
  
  
  METHODS
  Forty Sprague-Dawley rats were randomized into normal control, normal saline (10 mL/kg), model and ketamine+ EA groups. Ketamine-addiction model was established by intraperitoneal administration of ketamine (100 mg/kg) , once a day for 7 days. EA (2 Hz) was applied to "Zusanli" (ST 36) and "Sanyinjiao"(SP 6) for 30 min, once a day for 7 days. The expression of tyrosine hydroxylase (TH) and c-fos in the hippocampal CA 1 region was detected by immunohistochemistry.
  
  
  RESULTS
  In comparison with the normal control group and normal saline group, the numbers of both TH immune-reaction (IR)-positive and c-fos IR-positive neurons in the hippocampal region in the model group were increased significantly (P<0. 05, P<0. 01). Correspondingly, the expression of both TH IR-positive and c-fos IR-positive products in the hippocampal CA 1 region in the model group was upregulated evidently (P<0. 05, P<0. 01). After EA of "Zusanli" (ST 36)-"Sanyinjiao" (SP 6), compared with the model group, the expression of both TH IR-positive and c-fos IR-positive products was downregulated considerably (P<0. 01).
  
  
  CONCLUSIONS
  EA of "Zusanli"(ST 36)-"Sanyinjiao" (SP 6) can downregulate ketamine-addiction induced increase of expression of tyrosine hydroxylase and c-fos in the hippocampal CA 1 region in ketamine-addiction rats, which may contribute to its effect in relieving ketamine addiction symptoms in clinic.
authors.affiliation: Department of Anatomy, Wannan Medical College, Wuhu 241002, China.
authors.forename: Li-fa
authors.initials: LF
authors.lastname: Wang
authors.forename: Chen-li
authors.initials: CL
authors.lastname: Xu
authors.forename: Ke-ren
authors.initials: KR
authors.lastname: Xiong
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D015671:Electroacupuncture; D005260:Female; D006624:Hippocampus / Q000378:metabolism*; D006801:Humans; D007649:Ketamine / Q000009:adverse effects*; D008297:Male; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics* / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D019966:Substance-Related Disorders / Q000235:genetics* / Q000378:metabolism / Q000628:therapy*; D014446:Tyrosine 3-Monooxygenase / Q000235:genetics* / Q000378:metabolism
chemical_list: D016760:Proto-Oncogene Proteins c-fos; D007649:Ketamine; D014446:Tyrosine 3-Monooxygenase