title: The cbbL gene is required for thiosulfate-dependent autotrophic growth of Bradyrhizobium japonicum.
abstract: Bradyrhizobium japonicum is a facultative chemolithoautotroph capable of using thiosulfate and H(2) as an electron donor and CO(2) as a carbon source. In B. japonicum USDA110, the mutant of cbbL gene encoding a large subunit of ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO) was unable to grow using thiosulfate and H(2) as an electron donor. The cbbL deletion mutant was able to grow and oxidize thiosulfate in the presence of succinate. These results showed that the major route of CO(2) fixation for thiosulfate-dependent chemoautotrophic growth is the Calvin-Benson-Bassham cycle involving RuBisCO in B. japonicum.
authors.affiliation: Graduate School of Life Sciences, Tohoku University, 2–1–1 Katahira, Aoba-ku, Sendai 980–8577, Japan. sachiko@ige.tohoku.ac.jp
authors.forename: Sachiko
authors.initials: S
authors.lastname: Masuda
authors.forename: Shima
authors.initials: S
authors.lastname: Eda
authors.forename: Chiaki
authors.initials: C
authors.lastname: Sugawara
authors.forename: Hisayuki
authors.initials: H
authors.lastname: Mitsui
authors.forename: Kiwamu
authors.initials: K
authors.lastname: Minamisawa
mesh_terms: D052818:Autotrophic Processes; D020369:Bradyrhizobium / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D002245:Carbon Dioxide / Q000378:metabolism; D055786:Gene Knockout Techniques; D006859:Hydrogen / Q000378:metabolism; D010788:Photosynthesis / Q000235:genetics; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics* / Q000378:metabolism*; D019802:Succinic Acid / Q000378:metabolism; D013885:Thiosulfates / Q000378:metabolism*
chemical_list: D013885:Thiosulfates; D002245:Carbon Dioxide; D006859:Hydrogen; D019802:Succinic Acid; D012273:Ribulose-Bisphosphate Carboxylase