title: [Construction and confirmation of a recombinant adenovirus vector of survivin].
abstract: OBJECTIVE
  To construct a recombinant adenovirus of survivin vector and provid valuable reference for gene therapy of laryngeal cancer.
  
  
  METHODS
  The survivin gene was cloned by PCR. After confirmation by enzyme restriction analysis and sequencing, the gene and the adenovirus vector were recombined together to construct the recombinant adenovirus vector. The recombinant adenovirus vector was confirmed via both sequencing and digestion restriction analysis, and then linearized and transfected into the HEK 293 cell line to generate recombinant adenovirus.
  
  
  RESULTS
  The sequence analysis demonstrated that the survivin gene sequence was the same as published in the literature, suggesting that a recombinant adenovirus vector has been successfully constructed.
  
  
  CONCLUSIONS
  A survivin recombinant adenovirus has been successfully constructed.
authors.affiliation: Department of Head and Neck Surgery, The Third Affiliated Hospital of Harbin Medical University, Harbin 150081, China.
authors.forename: Yuan-Jing
authors.initials: YJ
authors.lastname: Lü
authors.forename: Su-Sheng
authors.initials: SS
authors.lastname: Miao
authors.forename: Wei-Song
authors.initials: WS
authors.lastname: Liu
authors.forename: Hong-Jiang
authors.initials: HJ
authors.lastname: He
authors.forename: Shen-Shan
authors.initials: SS
authors.lastname: Jia
authors.forename: Guo-Qing
authors.initials: GQ
authors.lastname: He
authors.forename: Cheng
authors.initials: C
authors.lastname: Xiang
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D005822:Genetic Vectors; D057809:HEK293 Cells; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000235:genetics* / Q000378:metabolism; D000077022:Survivin; D014162:Transfection
chemical_list: C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D011993:Recombinant Fusion Proteins; D000077022:Survivin