title: Development of polymorphic markers from expressed sequence tags of Manihot esculenta Crantz.
abstract: In this study, 49 primers were designed from sequences containing di-, tri-, tetra-, penta- and hexanucleotide motifs with a minimum of four repeats and presence of motif size polymorphisms (insertion/deletion) from cassava (Manihot esculenta Crantz) expressed sequence tags deposited in public sequence database. Each locus was subsequently screened on 29 M. esculenta Crantz obtained from 15 different countries. Cross-amplification was tested with M. esculenta Crantz (ssp. flabellifolia) and four different Manihot species, M. chlorosticta, M. carthaginensis, M. filamentosa and M. tristis. Of these, nine loci showed polymorphic profiles within M. esculenta Crantz, which revealed two to four alleles per locus. The average unbiased and direct count heterozygosities were 0.4901 and 0.5674, respectively.
authors.affiliation: National Center for Genetic Engineering and Biotechnology, 113 Phaholyothin Rd., Klong 1, Klong Luang, Pathumthani 12120, Thailand.
authors.forename: S
authors.initials: S
authors.lastname: Tangphatsornruang
authors.forename: S
authors.initials: S
authors.lastname: Sraphet
authors.forename: R
authors.initials: R
authors.lastname: Singh
authors.forename: E
authors.initials: E
authors.lastname: Okogbenin
authors.forename: M
authors.initials: M
authors.lastname: Fregene
authors.forename: K
authors.initials: K
authors.lastname: Triwitayakorn