title: RAPD-based genetic diversities and correlation with morphological traits in Camellia (Theaceae) cultivars in China.
abstract: Camellia is an economically important ornamental plant that has many uses, such as in beverages, foods and medicines. We examined 15 Camellia cultivars in Wenzhou, China, using RAPD markers and measurements of three traits (petal color, flower diameter, blooming period). PCR amplification with 15 random primers produced 1935 bands, observed at 88 amplification loci; 77% of the amplified loci were polymorphic, with a mean of 4.5 polymorphic loci per primer. The similarity coefficient ranged from 0.5419 to 0.7933 among the 15 samples; the lowest value was between Manao (C. reticulata) and Feibai FR (C. japonica), and the largest value was between Chidan (C. japonica) and Yuanyang FG (C. japonica). Cluster analysis divided the 15 cultivars into two groups at the similarity coefficient of 0.65. A correlation was found between RAPD markers and petal color in the first group. No correlation was found between RAPD markers and the other traits (flower diameter, blooming period). This study provides information useful for the identification, classification, phylogenesis, and breeding of Camellia cultivars.
authors.affiliation: College of Life and Environmental Sciences, Wenzhou University, Wenzhou, Zhejiang, PR China. wxf8080@126.com
authors.forename: X F
authors.initials: XF
authors.lastname: Wang
authors.forename: H Y
authors.initials: HY
authors.lastname: Zheng
authors.forename: W H
authors.initials: WH
authors.lastname: Zheng
authors.forename: C Q
authors.initials: CQ
authors.lastname: Ao
authors.forename: H Y
authors.initials: HY
authors.lastname: Jin
authors.forename: L H
authors.initials: LH
authors.lastname: Zhao
authors.forename: N
authors.initials: N
authors.lastname: Li
authors.forename: L R
authors.initials: LR
authors.lastname: Jia
mesh_terms: D028244:Camellia / Q000033:anatomy & histology* / Q000235:genetics*; D002681:China; D016000:Cluster Analysis; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D014644:Genetic Variation; D010641:Phenotype; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique
chemical_list: D017931:DNA Primers; D018744:DNA, Plant