title: PERMANENT GENETIC RESOURCES: Isolation and characterization of microsatellite loci from the European corn borer, Ostrinia nubilalis (Hübner) (Insecta: Lepidoptera: Crambidae).
abstract: Few useful microsatellites are available for population studies of the European corn borer, Ostrinia nubilalis (Hübner). An enrichment strategy was used to develop microsatellite markers for O. nubilalis, and over 500 positive clones were isolated. Seventy-five contained unique microsatellites, 10 of which were polymorphic with discernable polymerase chain reaction products. The 10 loci were surveyed for variability in 72 wild individuals from central Iowa. Five loci showed no deviation from Hardy-Weinberg proportions, and all were successfully cross-amplified in the related Asian corn borer, Ostrinia furnacalis. These loci represent a significant addition to microsatellites appropriate for population studies of O. nubilalis.
authors.affiliation: USDA-ARS, Corn Insects and Crop Genetics Research Unit, Genetics Laboratory, Iowa State University, Ames, IA 50011, USA.
authors.forename: Kyung Seok
authors.initials: KS
authors.lastname: Kim
authors.forename: Brad S
authors.initials: BS
authors.lastname: Coates
authors.forename: Richard L
authors.initials: RL
authors.lastname: Hellmich
authors.forename: Douglas V
authors.initials: DV
authors.lastname: Sumerford
authors.forename: Thomas W
authors.initials: TW
authors.lastname: Sappington