title: Quantification of human serum procollagen C-proteinase enhancer (hsPCPE) glycopattern.
abstract: BACKGROUND
  Procollagen C-proteinase enhancer 1 (PCPE1), a glycoprotein secreted from differentiating osteoblast, enhances the rate-limiting step of collagen type I fibrillar formation. It is expressed and secreted by cells that produce collagen type I and has the potential to be a marker for bone pathologies.
  
  
  METHODS
  We developed an assay to quantify PCPE glycopattern based on isoelectric focusing (IEF) and detection with a bio-imaging camera (coefficient of variation within and between assays, 15% and 20%, respectively).
  
  
  RESULTS
  PCPE was quantified in 39 serum samples from healthy subjects (17 females and 22 males). The concentration in the serum was 305(274) ng/ml, median(IQR). The level of the PCPE isoforms and their relative distribution were altered in patients with bone disorders.
  
  
  CONCLUSIONS
  The data generated by our system, support our hypothesis that combined data on PCPE concentration and isoforms may be useful for the diagnosis and follow-up of bone diseases. Further research, on larger cohorts of both normal subjects and patients, must be done.
authors.affiliation: Metabolic Enzymatic Laboratory, Chaim Sheba Medical Center, Tel Hashomer, Israel.
authors.forename: Yael
authors.initials: Y
authors.lastname: Olswang-Kuz
authors.forename: Boaz
authors.initials: B
authors.lastname: Liberman
authors.forename: Israel
authors.initials: I
authors.lastname: Weiss
authors.forename: Eyal
authors.initials: E
authors.lastname: Ramu
authors.forename: Rony
authors.initials: R
authors.lastname: Weitzen
authors.forename: Iris
authors.initials: I
authors.lastname: Vered
authors.forename: Galia
authors.initials: G
authors.lastname: Gat-Yablonski
authors.forename: Efrat
authors.initials: E
authors.lastname: Kessler
authors.forename: Yair
authors.initials: Y
authors.lastname: Anikster
authors.forename: Shlomit
authors.initials: S
authors.lastname: Mesilaty-Gross
mesh_terms: D000328:Adult; D001851:Bone Diseases, Metabolic / Q000097:blood; D002241:Carbohydrates / Q000737:chemistry*; D002460:Cell Line; D016326:Extracellular Matrix Proteins / Q000097:blood* / Q000737:chemistry; D005260:Female; D006023:Glycoproteins / Q000097:blood* / Q000737:chemistry; D006801:Humans; D007525:Isoelectric Focusing; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D015203:Reproducibility of Results
chemical_list: D002241:Carbohydrates; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; C491555:PCOLCE protein, human