title: Alternative splicing of human papillomavirus type-16 e6-messenger RNA in mouse and monkey cell-lines.
abstract: Previous studies have indicated that the splice patterns of E6-transcripts of human papillomavirus type-16 (HPV-16) are uniform. The splice ratios of E6-transcripts, however, seem to be variable in several HPV-positive cell lines, suggesting that cellular factors may affect the alternative splicing of E6-transcripts. To test this hypothesis, the splice ratios of E6-transcripts in various HPV-16 E6-expressing cell lines derived from CV-1 and 10T1/2 cells were quantitatively evaluated by S1 nuclease protection assays. The splice ratios varied among cell lines derived from the same parental lines, indicating that factors specific to cell-type do not play a major role in alternative splicing. The splice ratios appeared to be low in cell lines which prominently expressed longer than expected E6-transcripts indicating that the structure of the E6-transcript affects its splicing. Analysis of the expression patterns of COS-1 cells which transiently expressed various E6-transcript constructs suggested that structure was a factor in determining alternative splicing.
authors.affiliation: CHIBA UNIV,SCH MED,DEPT MICROBIOL,1-8-1 INOHANA,CHUO KU,CHIBA 260,JAPAN. CHIBA UNIV,SCH MED,DEPT OBSTET & GYNECOL,CHUO KU,CHIBA 260,JAPAN.
authors.forename: K
authors.initials: K
authors.lastname: Shimizu
authors.forename: H
authors.initials: H
authors.lastname: Shirasawa
authors.forename: M
authors.initials: M
authors.lastname: Jin
authors.forename: N
authors.initials: N
authors.lastname: Inaba
authors.forename: S
authors.initials: S
authors.lastname: Sekiya
authors.forename: B
authors.initials: B
authors.lastname: Simizu