title: Increased MLL gene rearrangements in amniocytes from fetuses of mothers who smoke.
abstract: We assess the possible genotoxic effect of maternal smoking on amniotic fluid cells, based on the presence of an increasing of structural abnormality of the 11q23 band bearing the MLL gene rearrangements. In this observational and prospective study cultured amniocytes were obtained from 20 control and 20 women who smoke (>10 cigarettes/day for >10 years and during pregnancy). We performed fluorescence in situ hybridization (FISH) analysis in amniocytes. Comparison of FISH data between smoker and control groups showed statistical significance for the MLL gene rearrangements. Epidemiologic studies, including a large series of patients, will be needed to determine whether the offspring of parents who smoke have an increased lifetime risk of leukemia.
authors.affiliation: Laboratori de Citogenètica Molecular, Servei de Patologia, Hospital del Mar, IMAS, Barcelona, Spain. rosaana.delachica@uab.cat
authors.forename: Rosa Ana
authors.initials: RA
authors.lastname: de la Chica
authors.forename: Carmen
authors.initials: C
authors.lastname: Mediano
authors.forename: Marta
authors.initials: M
authors.lastname: Salido
authors.forename: Blanca
authors.initials: B
authors.lastname: Espinet
authors.forename: Josep Maria
authors.initials: JM
authors.lastname: Manresa
authors.forename: Francesc
authors.initials: F
authors.lastname: Solé
mesh_terms: D000328:Adult; D000653:Amniotic Fluid / Q000166:cytology / Q000378:metabolism*; D016022:Case-Control Studies; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics*; D005260:Female; D005333:Fetus / Q000187:drug effects*; D015321:Gene Rearrangement / Q000187:drug effects*; D011495:Histone-Lysine N-Methyltransferase; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D009035:Mothers; D051788:Myeloid-Lymphoid Leukemia Protein / Q000235:genetics*; D011247:Pregnancy; D011379:Prognosis; D011446:Prospective Studies; D012907:Smoking / Q000009:adverse effects*
chemical_list: C497422:KMT2A protein, human; D051788:Myeloid-Lymphoid Leukemia Protein; D011495:Histone-Lysine N-Methyltransferase