title: Human prostate adenocarcinomas express in-vivo messenger-rnas and protein products for platelet-derived growth factor-B and its receptor.
abstract: In situ hybridization and immunocytochemistry studies have shown the in vivo expression of platelet-derived growth factor B (PDGF-B) and PDGF receptor (PDGF-R) beta mRNAs and their respective protein products in the malignant epithelial cells of eight primary human prostatic adenocarcinomas. Examination of five nonmalignant adjacent prostate tissues did not demonstrate significant expression of PDGF B and PDGF-R beta mRNAs or production of their respective protein products in nonmalignant epithelial cells. Expression of androgen receptor mRNA was shown to be present in the epithelial cells of all of the five nonmalignant adjacent prostate tissues. There was a significant reduction in the expression of androgen receptor mRNA in poorly differentiated regions, and a moderate reduction in the well differentiated regions of the malignant tissues. It appears that dedifferentiation of the tumor cells in prostatic adenocarcinomas is accompanied by a reduction in androgen receptor mRNA expression. The coexpression of PDGF and its receptor in the malignant epithelial cells of prostatic adenocarcinomas signifies an abnormal autocrine loop that may contribute to their growth and maintenance.
authors.affiliation: HARVARD UNIV,SCH PUBL HLTH,CTR BLOOD RES,800 HUNTINGTON AVE,BOSTON,MA 02115. HARVARD UNIV,SCH PUBL HLTH,DEPT CANC BIOL,BOSTON,MA 02115. HARVARD UNIV,SCH PUBL HLTH,DEPT NUTR,BOSTON,MA 02115. BRIGHAM & WOMENS HOSP,DEPT UROL,BOSTON,MA 02115. HARVARD UNIV,SCH MED,DEPT SURG,BOSTON,MA 02115.
authors.forename: M
authors.initials: M
authors.lastname: Xiao
authors.forename: T
authors.initials: T
authors.lastname: Galanopoulos
authors.forename: J
authors.initials: J
authors.lastname: Nevillegolden
authors.forename: J
authors.initials: J
authors.lastname: Richie
authors.forename: H
authors.initials: H
authors.lastname: Antoniades