title: LPS structure influences protein secretion in Salmonella enterica.
abstract: In this study we have compared protein secretion in the wild type of S. Typhimurium and the rfaC mutant. We found out that the rfaC mutant was defective in protein secretion. In addition, the rfaC mutant was defective in its invasion into an IPEC-J2 porcine epithelial cell line and also in motility in semisolid agar. Consistent with this, reduced flagella numbers were observed in the rfaC mutant. In the rfaC mutant, there were no defects in flagellin expression as detected by western blot and immune electron microscopy which demonstrated equal amounts of flagellin in the cytoplasm of both the rfaC mutant and the wild-type S. Typhimurium. However, in the wild-type strain only, the flagellin was assembled to spatially restricted areas on the inner side of cytoplasmic membrane. The oligosaccharide core of LPS is therefore required for the assembly of flagella and T3SS secretion machinery followed by protein secretion.
authors.affiliation: Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic.
authors.forename: M
authors.initials: M
authors.lastname: Crhanova
authors.forename: M
authors.initials: M
authors.lastname: Malcova
authors.forename: M
authors.initials: M
authors.lastname: Mazgajova
authors.forename: D
authors.initials: D
authors.lastname: Karasova
authors.forename: A
authors.initials: A
authors.lastname: Sebkova
authors.forename: A
authors.initials: A
authors.lastname: Fucikova
authors.forename: Z
authors.initials: Z
authors.lastname: Bortlicek
authors.forename: L
authors.initials: L
authors.lastname: Pilousova
authors.forename: K
authors.initials: K
authors.lastname: Kyrova
authors.forename: M
authors.initials: M
authors.lastname: Dekanova
authors.forename: I
authors.initials: I
authors.lastname: Rychlik
mesh_terms: D000818:Animals; D058947:Bacterial Secretion Systems; D002460:Cell Line; D003593:Cytoplasm / Q000737:chemistry; D004847:Epithelial Cells / Q000382:microbiology; D005407:Flagella / Q000378:metabolism*; D005408:Flagellin / Q000096:biosynthesis / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000737:chemistry*; D016253:Microscopy, Immunoelectron; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D019779:Salmonella enterica / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D013552:Swine
chemical_list: D058947:Bacterial Secretion Systems; D008070:Lipopolysaccharides; D005408:Flagellin