title: Iron oxide nanoparticle coating of organic polymer-based monolithic columns for phosphopeptide enrichment.
abstract: A new monolithic capillary column with an iron oxide nanoparticle coating has been developed for selective and efficient enrichment of phosphopeptides. Iron oxide nanoparticles were prepared by a co-precipitation method and stabilized by citrate ions. A stable coating of nanoparticles was obtained via multivalent electrostatic interactions of citrate ions on the surface of iron oxide nanoparticles with a quaternary amine functionalized poly(glycidyl methacrylate-co-ethylene dimethacrylate) monolith. A high dynamic binding capacity of 86 μmol/mL column volume was measured with an adenosine-5'-triphosphate. Performance of the monolithic column was demonstrated with the efficient and selective enrichment of phosphopeptides from peptide mixtures of α-casein and β-casein digests and their MALDI/MS characterization in off-line mode.
authors.affiliation: Institute of Analytical Chemistry of the ASCR, v. v. i., Veveri, Brno, Czech Republic. krenkova@iach.cz.
authors.forename: Jana
authors.initials: J
authors.lastname: Krenkova
authors.forename: Frantisek
authors.initials: F
authors.lastname: Foret
mesh_terms: D002364:Caseins / Q000737:chemistry; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D005290:Ferric Compounds / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry; D010748:Phosphopeptides / Q000737:chemistry / Q000302:isolation & purification*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D002364:Caseins; D005290:Ferric Compounds; D010748:Phosphopeptides; D011108:Polymers; C000499:ferric oxide