title: Identification of Helicobacter pylori strain cagPAI+ and cagPAI- Antigens by IgG antibodies from sera of experimentally colonized meriones unguiculatus (Mongolian gerbils).
abstract: BACKGROUND
  Mongolian gerbils that are experimentally infected with Helicobacter pylori develop a chronic inflammation that is similar to natural infections in humans. The aim of this study was to compare the antigens of H. pylori cagPAI+ and cagPAI- strains that are expressed during Meriones unguiculatus colonization.
  
  
  METHODS
  We identified H. pylori cagPAI+ and cagPAI- strain antigens via Western blotting of samples from Mongolian gerbils that were subjected to unique, mixed, and sequential bacterial infections.
  
  
  RESULTS
  The antigens from the J99/CG3 (cagPAI+) strain had a lower molecular weight than the antigens from the 251F/CG3 (cagPAI-) strain. There were fewer identified antigens in the single unique infections compared with the mixed and sequential infections. The number of recognized antigens that had a frequency of recognition >60% was higher for the simultaneous and sequential infection groups compared with the single infection group. A 57-kDa antigen was present in >60% of the samples and four of the five experimental groups. Antigens specific to each bacterial strain were identified; the 190- and 158-kDa antigens appear to be specific for cagPAI-, and the 70-kDa antigen appears to be specific for cagPAI+.
  
  
  CONCLUSIONS
  In this study, we identified antigens that are common and specific to the H. pylori cagPAI+ and cagPAI- strains.
authors.affiliation: Laboratory of Experimental Bacteriology, National Institute of Pediatrics, Mexico City, Mexico.
authors.forename: Carmen
authors.initials: C
authors.lastname: Zárate-Aquino
authors.forename: Jaime
authors.initials: J
authors.lastname: Torres-Marcial
authors.forename: Maribel
authors.initials: M
authors.lastname: Ortiz-Herrera
authors.forename: Héctor
authors.initials: H
authors.lastname: Romero-Ramírez
authors.forename: Leopoldo
authors.initials: L
authors.lastname: Santos-Argumedo
authors.forename: Eduardo
authors.initials: E
authors.lastname: López-Corella
authors.forename: Rafael
authors.initials: R
authors.lastname: Coria-Jiménez
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology*; D000942:Antigens, Bacterial / Q000737:chemistry / Q000276:immunology*; D015153:Blotting, Western; D004195:Disease Models, Animal; D044404:Genomic Islands; D005849:Gerbillinae; D016481:Helicobacter Infections / Q000276:immunology* / Q000382:microbiology; D016480:Helicobacter pylori / Q000737:chemistry / Q000276:immunology* / Q000502:physiology; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology*; D008297:Male; D008970:Molecular Weight; D011817:Rabbits
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007074:Immunoglobulin G