title: An easy and practical method for detection and estimation of microcrystalline cellulose in pasteurized milk.
abstract: Microcrystalline cellulose (MCC) is suspected to be a new adulteration in pasteurized milk in China, yet an efficient method for MCC detection in dairy has not been established. This study presents a novel procedure to detect and estimate MCC in pasteurized milk using dialysis, cellulase hydrolysis, and a reducing sugar assay. The background value of reducing sugar was eliminated by dialysis, and cellulase activity toward MCC was stable in dialyzed milk. A criterion for MCC detection and an empirical formula for MCC estimation were summarized based on the reducing sugar variation after hydrolysis. The detection sensitivity was below 0.5 g/L. Reducing sugar distribution after cellulase-catalyzed hydrolysis was examined by HPLC, and revealed that most of the detected sugar was glucose. This paper describes a practical method for detection of MCC in pasteurized milk that might benefit dairy QC.
authors.affiliation: National Supervision & Testing Centre for Agricultural & Sideline Product Quality, 168 Hongshan Rd, Nanjing, Jiangsu, People's Republic of China 210028. wuwu3660@sina.com
authors.forename: Chi
authors.initials: C
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.forename: Ling
authors.initials: L
authors.lastname: Qiao
mesh_terms: D000818:Animals; D002417:Cattle; D002480:Cellulase / Q000378:metabolism; D002482:Cellulose / Q000737:chemistry* / Q000378:metabolism; D003956:Dialysis; D006868:Hydrolysis; D008892:Milk / Q000737:chemistry*
chemical_list: D002482:Cellulose; D002480:Cellulase; C109691:microcrystalline cellulose