title: High-performance liquid chromatography with photodiode array detection for determination of nobiletin content in the brain and serum of mice administrated the natural compound.
abstract: We recently demonstrated that nobiletin, a citrus flavonoid, exhibits anti-dementia action in animals. However, no determination methods for the content of nobiletin with beneficial action in the brain of nobiletin-administered animals have been developed, nor has its pharmacokinetics been revealed completely. Here, we established the high-performance liquid chromatography/photodiode array detection method for nobiletin determination using Bond Elut C18 SPE cartridges for extraction, where the calibration curve was linear over 0.025-10 ng, with coefficient of variation of less than 6.76%. This method enabled us to determine pharmacokinetic parameters of nobiletin given intraperitoneally or per os in the brain of mice.
authors.affiliation: Laboratory of Oncology Pharmacy Practice and Sciences, Graduate School of Pharmaceutical Science, Tohoku University, Aoba-ku, Sendai, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Saigusa
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Shibuya
authors.forename: Daisuke
authors.initials: D
authors.lastname: Jinno
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Yamakoshi
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Iwabuchi
authors.forename: Akihito
authors.initials: A
authors.lastname: Yokosuka
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Mimaki
authors.forename: Akira
authors.initials: A
authors.lastname: Naganuma
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Ohizumi
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Tomioka
authors.forename: Tohru
authors.initials: T
authors.lastname: Yamakuni
mesh_terms: D000818:Animals; D000975:Antioxidants; D001921:Brain / Q000378:metabolism*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D047309:Flavones / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D005419:Flavonoids; D051379:Mice; D015203:Reproducibility of Results
chemical_list: D000975:Antioxidants; D047309:Flavones; D005419:Flavonoids; C008661:nobiletin