title: [Identification of the offspring of Vegfr2-luc transgenic mouse].
abstract: OBJECTIVE
  A transgenic mouse, Vegfr2-luc, in which a luciferase reporter (luc) is under control of the murine VEGFR2 promoter, can be used to track angiogenesis in vivo. The aim of this study is to identify the offspring of Vegfr2-luc transgenic mouse.
  
  
  METHODS
  Luc was detected with PCR in genomic DNA of the new-born mouse. Luc expression in the offspring of Vegfr2-luc transgenic mouse was monitored with IVIS in vivo imaging system during post-natal development. Wound-healing models of Vegfr2-luc transgenic mouse offspring were established and the expression of luc was monitored during the wound-healing process. Luc activity and VEGFR2 mRNA expression in different organs were detected with luc Assay System and Real-time PCR respectively.
  
  
  RESULTS
  PCR showed that 50% (56/112) of the offspring of Vegfr2-luc transgenic mouse carry luc. IVIS in vivo imaging results demonstrated that luc expression in Vegfr2-luc transgenic mouse dropped dramatically with age increase (P<0.001) and luc expression in the wound first increased and then decreased during the wound-healing process (P<0.001). Luc activity in female Vegfr2-luc transgenic mouse organs was positively correlated with VEGFR2 mRNA expression (r=0.948, P<0.001). Except testis, luc activity in male Vegfr2-luc transgenic mouse organs was also positively correlated with VEGFR2 mRNA expression (r=0.836, P<0.001).
  
  
  CONCLUSIONS
  The offspring of Vegfr2-luc transgenic mouse is applicable to tracking angiogenesis in vivo.
authors.affiliation: Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenviroment, Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin 300052, China.
authors.forename: Weiqiang
authors.initials: W
authors.lastname: Wang
authors.forename: Hongyu
authors.initials: H
authors.lastname: Liu
authors.forename: Zhitao
authors.initials: Z
authors.lastname: Song
authors.forename: Yuli
authors.initials: Y
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.forename: Yongwen
authors.initials: Y
authors.lastname: Li
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Zhou
mesh_terms: D000818:Animals; D005260:Female; D017930:Genes, Reporter; D008156:Luciferases / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008822:Mice, Transgenic / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000235:genetics* / Q000378:metabolism; D014945:Wound Healing
chemical_list: D008156:Luciferases; D040301:Vascular Endothelial Growth Factor Receptor-2