title: Real time observation of mouse fetal skeleton using a high resolution X-ray synchrotron.
abstract: The X-ray synchrotron is quite different from conventional radiation sources. This technique may expand the capabilities of conventional radiology and be applied in novel manners for special cases. To evaluate the usefulness of X-ray synchrotron radiation systems for real time observations, mouse fetal skeleton development was monitored with a high resolution X-ray synchrotron. A non-monochromatized X-ray synchrotron (white beam, 5C1 beamline) was employed to observe the skeleton of mice under anesthesia at embryonic day (E)12, E14, E15, and E18. At the same time, conventional radiography and mammography were used to compare with X-ray synchrotron. After synchrotron radiation, each mouse was sacrificed and stained with Alizarin red S and Alcian blue to observe bony structures. Synchrotron radiation enabled us to view the mouse fetal skeleton beginning at gestation. Synchrotron radiation systems facilitate real time observations of the fetal skeleton with greater accuracy and magnification compared to mammography and conventional radiography. Our results show that X-ray synchrotron systems can be used to observe the fine structures of internal organs at high magnification.
authors.affiliation: Department of Radiology, College of Veterinary Medicine, Chungbuk National University, Cheongju 361-763, Korea.
authors.forename: Dong Woo
authors.initials: DW
authors.lastname: Chang
authors.forename: Bora
authors.initials: B
authors.lastname: Kim
authors.forename: Jae Hoon
authors.initials: JH
authors.lastname: Shin
authors.forename: Young Min
authors.initials: YM
authors.lastname: Yun
authors.forename: Jung Ho
authors.initials: JH
authors.lastname: Je
authors.forename: Yeu Kuang
authors.initials: YK
authors.lastname: Hwu
authors.forename: Jung Hee
authors.initials: JH
authors.lastname: Yoon
authors.forename: Je Kyung
authors.initials: JK
authors.lastname: Seong
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000033:anatomy & histology* / Q000000981:diagnostic imaging; D005260:Female; D005333:Fetus / Q000033:anatomy & histology* / Q000000981:diagnostic imaging; D006651:Histocytochemistry; D051379:Mice; D008813:Mice, Inbred ICR; D011247:Pregnancy; D011859:Radiography; D017356:Synchrotrons; D014965:X-Rays