title: Amino acid sequences of two immune-dominant epitopes of recoverin are involved in Ca2+/recoverin-dependent inhibition of phosphorylation of rhodopsin.
abstract: Antibodies AB(60-72) and AB(80-92) against two immune-dominant epitopes of photoreceptor Ca(2+)-binding protein recoverin, 60-DPKAYAQHVFRSF-72 and 80-LDFKEYVIALHMT-92, which can be exposed in a Ca(2+)-dependent manner, were obtained. The presence of AB(60-72) or AB(80-92) results in a slight increase in Ca(2+)-affinity of recoverin and does not affect significantly a Ca(2+)-myristoyl switch mechanism of the protein. However in the presence of AB(60-72) or AB(80-92) recoverin loses its ability to interact with rhodopsin kinase and consequently to perform a function of Ca(2+)-sensitive inhibitor of rhodopsin phosphorylation in photoreceptor cells.
authors.affiliation: Department of Cell Signaling, Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Russia. senin@belozersky.msu.ru
authors.forename: I I
authors.initials: II
authors.lastname: Senin
authors.forename: N K
authors.initials: NK
authors.lastname: Tikhomirova
authors.forename: V A
authors.initials: VA
authors.lastname: Churumova
authors.forename: I I
authors.initials: II
authors.lastname: Grigoriev
authors.forename: T A
authors.initials: TA
authors.lastname: Kolpakova
authors.forename: D V
authors.initials: DV
authors.lastname: Zinchenko
authors.forename: P P
authors.initials: PP
authors.lastname: Philippov
authors.forename: E Yu
authors.initials: EY
authors.lastname: Zernii
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology; D000918:Antibody Specificity; D002118:Calcium / Q000378:metabolism*; D002417:Cattle; D051606:G-Protein-Coupled Receptor Kinase 1 / Q000037:antagonists & inhibitors; D016056:Immunodominant Epitopes / Q000737:chemistry* / Q000276:immunology* / Q000378:metabolism; D019814:Myristic Acid / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D047428:Protein Kinase Inhibitors / Q000737:chemistry / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology; D051603:Recoverin / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism* / Q000494:pharmacology; D012243:Rhodopsin / Q000378:metabolism*
chemical_list: D000906:Antibodies; D016056:Immunodominant Epitopes; D047428:Protein Kinase Inhibitors; D019814:Myristic Acid; D051603:Recoverin; D012243:Rhodopsin; D051606:G-Protein-Coupled Receptor Kinase 1; D002118:Calcium