title: An ENDOR and HYSCORE investigation of a reaction intermediate in IspG (GcpE) catalysis.
abstract: IspG is a 4Fe-4S protein that carries out an essential reduction step in isoprenoid biosynthesis. Using electron-nuclear double resonance (ENDOR) and hyperfine sublevel correlation (HYSCORE) spectroscopies on labeled samples, we have specifically assigned the hyperfine interactions in a reaction intermediate. These results help clarify the nature of the reaction intermediate, supporting a direct interaction between the unique fourth Fe in the cluster and C2 and O3 of the ligand.
authors.affiliation: Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign, 607 South Mathews Avenue, Urbana, Illinois 61801, USA.
authors.forename: Weixue
authors.initials: W
authors.lastname: Wang
authors.forename: Ke
authors.initials: K
authors.lastname: Wang
authors.forename: Jikun
authors.initials: J
authors.lastname: Li
authors.forename: Saritha
authors.initials: S
authors.lastname: Nellutla
authors.forename: Tatyana I
authors.initials: TI
authors.lastname: Smirnova
authors.forename: Eric
authors.initials: E
authors.lastname: Oldfield
mesh_terms: D019883:Alkyl and Aryl Transferases / Q000096:biosynthesis* / Q000737:chemistry*; D002384:Catalysis; D004578:Electron Spin Resonance Spectroscopy; D004798:Enzymes / Q000737:chemistry*; D008958:Models, Molecular; D015394:Molecular Structure
chemical_list: D004798:Enzymes; C421242:hydroxymethylbutenyl 4-diphosphate synthase; D019883:Alkyl and Aryl Transferases