title: Genetic manipulation of pathway regulation for overproduction of angucycline-like antibiotic auricin in Streptomyces aureofaciens CCM 3239.
abstract: The polyketide gene cluster aur1 is responsible for the production of the antibiotic auricin in Streptomyces aureofaciens CCM 3239. Auricin production is low and strictly regulated by two regulators, Aur1P and Aur1R. To improve auricin yield, we genetically manipulated S. aureofaciens CCM 3239 strain to overcome this strict regulation. A regulatory region including aur1R, aur1P, aur1O and the target biosynthetic aur1Ap promoter were replaced by the strong constitutive ermEp* promoter. However, auricin production was decreased in such a genetically manipulated strain. In the second strategy we placed the aur1P gene for auricin pathway-specific activator under the control of the ermEp* promoter. The resulting strain has been shown to produce 2.8-fold higher amount of auricin compared with the WT strain.
authors.affiliation: Institute of Molecular Biology, Slovak Academy of Sciences, 845 51, Bratislava, Slovak Republic.
authors.forename: Renata
authors.initials: R
authors.lastname: Novakova
authors.forename: Alena
authors.initials: A
authors.lastname: Rehakova
authors.forename: Lubomira
authors.initials: L
authors.lastname: Feckova
authors.forename: Peter
authors.initials: P
authors.lastname: Kutas
authors.forename: Renata
authors.initials: R
authors.lastname: Knischova
authors.forename: Jan
authors.initials: J
authors.lastname: Kormanec
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D002851:Chromatography, High Pressure Liquid; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D018942:Macrolides / Q000378:metabolism*; D005810:Multigene Family; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D013304:Streptomyces aureofaciens / Q000235:genetics* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D018942:Macrolides; C505282:auricin