title: Microsatellite markers for the red band needle blight pathogen, Dothistroma septosporum.
abstract: Twelve microsatellite markers were developed for population analyses of the fungal pathogen, Dothistroma septosporum. Intersimple sequence repeat polymerase chain reaction (ISSR-PCR) and an enrichment protocol (fast isolation by amplified fragment length polymorphism of sequences containing repeats [FIASCO]) were both used to identify 28 unique microsatellite regions in the genome. From 22 primer pairs designed, 12 were polymorphic. These markers, screened on two populations representing 42 isolates, produced 40 alleles across all loci with an allelic diversity of 0.09-0.76 per locus. Cross-species amplification showed variable success with Dothistroma rhabdoclinis and Mycosphaerella dearnessi and some sequence variation within isolates of Dothistroma pini. These markers will be used to further study the population structure and diversity of D. septosporum.
authors.affiliation: Department of Genetics, Forestry and Agricultural Biotechnology Institute, University of Pretoria, Pretoria 0002, South Africa.
authors.forename: I
authors.initials: I
authors.lastname: Barnes
authors.forename: M N
authors.initials: MN
authors.lastname: Cortinas
authors.forename: M J
authors.initials: MJ
authors.lastname: Wingfield
authors.forename: B D
authors.initials: BD
authors.lastname: Wingfield