title: Evaluation of the immunomodulatory effect of the 14 kDa protein isolated from aged garlic extract on dendritic cells.
abstract: Garlic is used all over the world for treatment of different diseases. A wide range of biological activities of garlic has been verified in vitro and in vivo. One of major proteins of garlic which has been isolated and purified is the 14 kDa protein. This protein has been shown to have immunomodulatory effects. In this study, the effect of the 14 kDa protein isolated from aged garlic extract (AGE) was investigated on maturation and immunomodulatory activity of dendritic cells (DC). Proteins were purified from AGE by biochemical method; the semi-purified 14 kDa protein was run on gel filtration Sephadex G50 and its purity was checked by SDS-PAGE. DC were isolated from spleen of BALB/c mice by Nycodenz centrifugation and their adhesiveness to plastic dish. 14 kDa protein from AGE was added to overnight culture of DC medium and the expression percentage of CD40, CD86, and MHC-II was evaluated by flowcytometric analysis. Also, proliferation of T-cells was measured by allogenic mixed lymphocyte reaction (MLR) test. The purified 14 kDa protein isolated from AGE increased the expression of CD40 molecule on DC, but it did not influence CD86 and MHCII molecules. Furthermore, no significant differences were noticed in the pulsed-DC with 14 kDa protein and non-pulsed DC on the MLR.
authors.affiliation: Department of Immunology, School of Medicine, Tarbiat Modares University, Tehran, Iran.
authors.forename: Hasan Namdar
authors.initials: HN
authors.lastname: Ahmadabad
authors.forename: Zuhair Mohammad
authors.initials: ZM
authors.lastname: Hassan
authors.forename: Elahe
authors.initials: E
authors.lastname: Safari
authors.forename: Mahmood
authors.initials: M
authors.lastname: Bozorgmehr
authors.forename: Tooba
authors.initials: T
authors.lastname: Ghazanfari
authors.forename: Seyed Mohammad
authors.initials: SM
authors.lastname: Moazzeni
mesh_terms: D000818:Animals; D051940:B7-2 Antigen / Q000378:metabolism; D019013:CD40 Antigens / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005737:Garlic / Q000737:chemistry*; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D007155:Immunologic Factors / Q000276:immunology* / Q000302:isolation & purification / Q000494:pharmacology; D008198:Lymph Nodes / Q000166:cytology; D008213:Lymphocyte Activation / Q000276:immunology; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010936:Plant Extracts / Q000737:chemistry* / Q000276:immunology; D014674:Plant Proteins, Dietary / Q000276:immunology* / Q000302:isolation & purification / Q000494:pharmacology; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology
chemical_list: D051940:B7-2 Antigen; D019013:CD40 Antigens; C497965:Cd86 protein, mouse; D000949:Histocompatibility Antigens Class II; D007155:Immunologic Factors; D010936:Plant Extracts; D014674:Plant Proteins, Dietary