title: The effects of tumbling and sodium tripolyphosphate on the proteins of döner.
abstract: This study was designed to investigate the influence of tumbling and sodium tripolyphosphate (STPP) with marination on the protein structure of raw and cooked döner, a traditional Middle East product consumed widely in many areas of the world. Proximate composition (%moisture, %protein, %fat, %ash, and pH value), salt soluble protein (SSP) and total α-amino grup amount were determined. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was used to investigate alterations in the sarcoplasmic and myofibrillar proteins. Utilization of STPP increased the moisture content and ash contents, and pH value of raw döner (p<0.05). Only tumbling and STPP had a significant two-way interaction for moisture content, SSP and total α-amino group amounts of cooked döner (p<0.05). None of the treatments significantly affected the fraction of sarcoplasmic and myofibrillar proteins. In addition, cooking caused proteolysis of these proteins.
authors.affiliation: Ankara University, Engineering Faculty, Food Engineering Department, Dışkapı Campus 14/C, 06110 Ankara, Turkey. edemirok@eng.ankara.edu.tr
authors.forename: Eda
authors.initials: E
authors.lastname: Demirok
authors.forename: Nuray
authors.initials: N
authors.lastname: Kolsarıcı
authors.forename: İlker Turan
authors.initials: İT
authors.lastname: Akoğlu
authors.forename: Esma
authors.initials: E
authors.lastname: Özden
mesh_terms: D000818:Animals; D002417:Cattle; D003296:Cooking; D004591:Electrophoresis, Polyacrylamide Gel; D005223:Fats / Q000032:analysis / Q000737:chemistry; D005511:Food Handling / Q000379:methods*; D005520:Food Preservatives / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D008461:Meat Products; D008877:Middle East; D009124:Muscle Proteins / Q000032:analysis / Q000737:chemistry* / Q000187:drug effects; D009210:Myofibrils / Q000737:chemistry / Q000187:drug effects; D011122:Polyphosphates / Q000494:pharmacology*; D012519:Sarcoplasmic Reticulum / Q000737:chemistry / Q000187:drug effects
chemical_list: D005223:Fats; D005520:Food Preservatives; D009124:Muscle Proteins; D011122:Polyphosphates; C005692:triphosphoric acid