title: Accurate and highly sensitive screening of DNA-binding molecules with primer-dimer templates using a real-time PCR analyzer.
abstract: We have developed a strategy to detect the DNA-binding affinities of chemical compounds with a real-time PCR analyzer. In this system, the DNA-binding affinities of chemical compounds are efficiency detected by PCR with high sensitivity. To obtain accurate information about the DNA-binding affinities of chemical compounds, primer-dimers prepared in advance are used as templates. The denature temperature is settled to around the melting temperature of the templates.
authors.affiliation: Faculty of Pharmaceutical Sciences, Tokyo University of Science, Chiba, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Inoue
authors.forename: Tsunehiro
authors.initials: T
authors.lastname: Kumeda
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nakamura
mesh_terms: D004247:DNA / Q000737:chemistry* / Q000187:drug effects / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics*; D019281:Dimerization; D007211:Indoles / Q000032:analysis* / Q000737:chemistry* / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D013696:Temperature
chemical_list: D017931:DNA Primers; D007211:Indoles; C007293:DAPI; D004247:DNA