title: Ha-ras codon 12 mutation in papillary tumors of the urinary-bladder - a retrospective study.
abstract: This report concerns the retrospective examination of low grade, low stage (Ta/T1) bladder tumors for the Ha-ras codon 12 G-->T mutation. The patients studied had a minimum of 5 years follow-up and were grouped into 3 categories: (i) patients with no recurring tumors, (ii) patients with recurring Ta/T1 tumors and (iii) patients who subsequently developed high grade, high stage tumors. A heminested, non-isotopic, allele-specific polymerase chain reaction (PCR) amplification assay was used. The codon 12 G-->T mutation was found in 10/27 specimens from patients with non-recurring Ta/T1 tumors; in 11/27 initial and 12/23 recurring Ta/T1 tumors, and in 5/8 initial Ta/T1 lesions and 8/12 subsequently developed high grade/stage tumors. Although there was no correlation between disease recurrence and mutation, these results indicate that a relatively large proportion of patients with Ta/T1 tumors of the bladder have cells with the Ha-ras codon 12 G-->T substitution.
authors.affiliation: MONTEFIORE MED CTR,ALBERT EINSTEIN COLL MED,DEPT PATHOL,BRONX,NY 10467. MEM SLOAN KETTERING CANC CTR,DEPT PATHOL,NEW YORK,NY 10021. UNIV LAVAL,CANC RES CTR,DEPT SURG UROL,QUEBEC CITY G1R 2J6,PQ,CANADA. UNIV CALIF SAN FRANCISCO,DEPT LAB MED,SAN FRANCISCO,CA 94143.
authors.forename: A
authors.initials: A
authors.lastname: Ooi
authors.forename: F
authors.initials: F
authors.lastname: Herz
authors.forename: S
authors.initials: S
authors.lastname: Ii
authors.forename: C
authors.initials: C
authors.lastname: Cordoncardo
authors.forename: Y
authors.initials: Y
authors.lastname: Fradet
authors.forename: B
authors.initials: B
authors.lastname: Mayall