title: Development of nine microsatellite markers for Pomacentrus amboinensis.
abstract: The relatively long pelagic larval duration of Pomacentrus amboinensis, a tropical fish, suggests the potential for long-distance dispersal; however, several nongenetic studies have found substantial self-recruitment at one location. To analyse patterns of connectivity of this species, primers for nine independent microsatellite loci were developed for P. amboinensis using a magnetic bead enrichment protocol. Twenty individuals from one location were analysed and observed heterozygosities ranged from 0.7 to 0.95. Eight of nine loci were in Hardy-Weinberg equilibrium and no evidence of linkage or null alleles were found.
authors.affiliation: School of Marine and Tropical Biology, James Cook University, Townsville, Qld 4811, Australia, ARC Centre of Excellence for Coral Reef Studies, Townsville, Qld 4811, Australia.
authors.forename: David B
authors.initials: DB
authors.lastname: Jones
authors.forename: Dean R
authors.initials: DR
authors.lastname: Jerry
authors.forename: Mark I
authors.initials: MI
authors.lastname: McCormick
authors.forename: Line K
authors.initials: LK
authors.lastname: Bay