title: Antioxidant effects of antioxidant biofactor on reactive oxygen species in human gingival fibroblasts.
abstract: The purpose of this study was to investigate the effects of antioxidant biofactor (AOB) on reactive oxygen species (ROS). Generation of superoxide radical (O(2) (•-)) and hydroxyl radical ((•)OH) was determined using an electron spin resonance (ESR) spin-trapping method. AOB was added at different concentrations to these free radical generating systems. The generation of both O(2) (•-) and (•)OH was scavenged by the addition of AOB in a dose-dependent manner. These results indicate that AOB has strong antioxidant properties against these radicals. We further investigated the anti-oxidative effect of AOB on human gingival fibroblasts (HGFs). HGFs were treated for 3 h with α-MEM containing a combination of AOB and H(2)O(2) (AOB + H(2)O(2) group), containing H(2)O(2) (H(2)O(2) group), or containing AOB alone (AOB group). Non-stimulated HGFs were used as a control group. The number of surviving cells was in the order of the AOB group > control group > AOB + H(2)O(2) group > H(2)O(2) group. The level of expression of type I collagen mRNA and production of collagen were also in the order of the AOB group > control group > AOB + H(2)O(2) group > H(2)O(2) group. In conclusion, our results suggest that AOB may protect HGFs against oxidative stress by reducing stress-induced ROS.
authors.affiliation: Department of Endodontics, Nihon University School of Dentistry at Matsudo, 870-1, Sakaecho, Nishi-2, Matsudo, Chiba 271-8587, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Matsui
authors.forename: Yasuhisa
authors.initials: Y
authors.lastname: Tsujimoto
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Ozawa
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Matsushima