title: Characterization of a new male sterility-related gene Camf1 in Capsicum annum L.
abstract: For the sake of screening novel genes related to the male sterility in chili pepper for studying the molecular mechanism of plant male sterility, the gene differential expression analysis was performed by cDNA-amplified fragment length polymorphism (cDNA-AFLP) in the genic male sterile-fertile line 114AB of Capsicum annum L., and a variety of differentially expressed cDNA fragments were detected in fertile or sterility material. Camf1, a transcript-derived fragment (TDF) accumulated in fertile line flower buds was further investigated. The Camf1 has 1,854 bp in length with no introns containing a 1,707-bp open reading frame (ORF). The deduced amino acid sequence of Camf1 shares higher similarity to some members from a glyoxal oxidase-related protein family, and has a glyoxal oxidase conserved domain at the N-terminus and a domain of unknown function (DUF1929) at C-terminal end. Expression analysis showed that Camf1 expressed only in stage 3-7 flower buds of male fertile of C. annum L. 114AB, and no detection in all organs of male sterility. The peak of expression level of Camf1 appeared at stage 4 flower buds of fertile line. Furthermore, expression analysis of different organs revealed that Camf1 expressed only in anthers of male fertile material and there were no expression in sepals, petals, pistils, roots, stems, leaves and open flowers. These results suggested that Camf1 was an anther-specific gene and might be essential for the fertility of C. annum L.
authors.affiliation: College of Horticulture, South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Chang Ming
authors.initials: CM
authors.lastname: Chen
authors.forename: Xue Feng
authors.initials: XF
authors.lastname: Hao
authors.forename: Guo Ju
authors.initials: GJ
authors.lastname: Chen
authors.forename: Bi Hao
authors.initials: BH
authors.lastname: Cao
authors.forename: Qing Hua
authors.initials: QH
authors.lastname: Chen
authors.forename: Shao Qun
authors.initials: SQ
authors.lastname: Liu
authors.forename: Jian Jun
authors.initials: JJ
authors.lastname: Lei
mesh_terms: D000429:Alcohol Oxidoreductases / Q000235:genetics; D054458:Amplified Fragment Length Polymorphism Analysis; D001483:Base Sequence; D002212:Capsicum / Q000235:genetics* / Q000502:physiology; D019295:Computational Biology; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D005298:Fertility / Q000235:genetics; D040461:Gene Components; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D017343:Genes, Plant / Q000235:genetics*; D008969:Molecular Sequence Data; D051479:Plant Infertility / Q000235:genetics* / Q000502:physiology; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D000429:Alcohol Oxidoreductases; C052371:glyoxal oxidase