title: Red and white wines inhibit cholesterol oxidation induced by free radicals.
abstract: The capabilities of two red (RW) and two white wines (WW) in inhibiting cholesterol oxidation were evaluated using a cholesterol emulsion (CE) system. Each RW or WW was mixed with CE at different (v/v) ratios. Cholesterol oxidation was accelerated by a free radical generator, 2,2'-azobis(2-methylpropionamidine) dihydrochloride (AAPH), at 37 °C. The major oxidation product, 7-ketocholesterol, was monitored to determine cholesterol stability in the CE system. At a ratio of 1:250 (RW/CE), 7-ketocholesterol production was not detected during 72 h of oxidation. At a 1:1000 ratio, the inhibition rate of each RW was maintained at 100% at 24 h but decreased afterward. Both WWs had 100% inhibition rate within 48 h at a ratio of 1:10. Also, the capabilities of catechin and resveratrol solutions (1 mg/mL) in inhibiting cholesterol oxidation were studied. Each of the wine polyphenolics showed a 100% of 7-ketocholesterol inhibition rate in 24 h at a ratio of 1:500 (solution/CE). However, the inhibition rate of resveratrol was lower than that of catechin at 48 or 72 h. The results demonstrated that red wine possesses great anti-cholesterol-oxidation capability, which may contribute to health benefits in preventing cardiovascular diseases. Catechin may play a more important role than resveratrol in inhibiting cholesterol oxidation.
authors.affiliation: College of Enology, Northwest A&F University , Shaanxi, Yangling 712100, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Tian
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.forename: Ahmed Moursy
authors.initials: AM
authors.lastname: Abdallah
authors.forename: Witoon
authors.initials: W
authors.lastname: Prinyawiwatkul
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Xu
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D002784:Cholesterol / Q000737:chemistry*; D015536:Down-Regulation; D005609:Free Radicals / Q000737:chemistry*; D010084:Oxidation-Reduction; D014920:Wine / Q000032:analysis*
chemical_list: D000975:Antioxidants; D005609:Free Radicals; D002784:Cholesterol