title: High dietary protein combats the stress of Labeo rohita fingerlings exposed to heat shock.
abstract: The amelioration effect of high dietary protein against stress was evaluated in Labeo rohita fingerlings, exposed to heat shock. Two hundred and forty fingerlings (6.57 ± 0.04 g, average weight ± SE) were randomly distributed into 4 treatment groups, each with 4 replicates was fed with either of four diets containing different levels of protein (20, 30, 40 or 45%). Water temperatures of all the treatments were within the range of 25.5-26.5°C throughout the experimental period of 30 days. After 30 days of feeding, fish were given heat shock by exposing to 38°C for 2 h. Heat shock significantly decreased (P < 0.05) liver glycogen content in treatment groups fed with 20 and 30% dietary protein, whereas unaffected in the 40 and 45% protein-fed groups. Heat shock significantly increased (P < 0.05) serum glucose and cortisol level in all the treatments. The 40 and 45% dietary protein-fed groups registered significantly higher survival (%) after the heat shock compared with their lower-protein counterparts. Heat shock increased the glycolytic, gluconeogenic, protein metabolic and antioxidative enzymes to cope up with thermal stress. Our results indicate that high-protein diet (≥40%) combats the stress due to heat shock in Labeo rohita.
authors.affiliation: Krishi Vigyan Kendra, Rajendra Agricultural University, Madhopur, Bihar, India. shivdholi@rediffmail.com
authors.forename: Shivendra
authors.initials: S
authors.lastname: Kumar
authors.forename: N P
authors.initials: NP
authors.lastname: Sahu
authors.forename: A K
authors.initials: AK
authors.lastname: Pal
authors.forename: Saravanan
authors.initials: S
authors.lastname: Subramanian
authors.forename: Himanshu
authors.initials: H
authors.lastname: Priyadarshi
authors.forename: Vikas
authors.initials: V
authors.lastname: Kumar
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D003530:Cyprinidae / Q000502:physiology*; D004044:Dietary Proteins; D006003:Glycogen / Q000378:metabolism; D018869:Heat-Shock Response; D006358:Hot Temperature; D006854:Hydrocortisone / Q000097:blood; D008099:Liver / Q000201:enzymology; D009132:Muscles / Q000378:metabolism
chemical_list: D001786:Blood Glucose; D004044:Dietary Proteins; D006003:Glycogen; D006854:Hydrocortisone