title: Angiotensin II induced catabolic effect and muscle atrophy are redox dependent.
abstract: Angiotensin II (Ang II) causes skeletal muscle wasting via an increase in muscle catabolism. To determine whether the wasting effects of Ang II were related to its ability to increase NADPH oxidase-derived reactive oxygen species (ROS) we infused wild-type C57BL/6J or p47(phox)(-/-) mice with vehicle or Ang II for 7days. Superoxide production was increased 2.4-fold in the skeletal muscle of Ang II infused mice, and this increase was prevented in p47(phox)(-/-) mice. Apocynin treatment prevented Ang II-induced superoxide production in skeletal muscle, consistent with Ang II increasing NADPH oxidase derived ROS. Ang II induced loss of body and skeletal muscle weight in C57BL/6J mice, whereas the reduction was significantly attenuated in p47(phox)(-/-) animals. The reduction of skeletal muscle weight caused by Ang II was associated with an increase of proteasome activity, and this increase was completely prevented in the skeletal muscle of p47(phox)(-/-) mice. In conclusion, Ang II-induced skeletal muscle wasting is in part dependent on NADPH oxidase derived ROS.
authors.affiliation: Heart and Vascular Institute, Tulane University School of Medicine, Tulane University Heart and Vascular Institute, 1430 Tulane Avenue, SL 48, New Orleans, LA 70112, United States.
authors.forename: Laura C
authors.initials: LC
authors.lastname: Semprun-Prieto
authors.forename: Sergiy
authors.initials: S
authors.lastname: Sukhanov
authors.forename: Tadashi
authors.initials: T
authors.lastname: Yoshida
authors.forename: Bashir M
authors.initials: BM
authors.lastname: Rezk
authors.forename: Romer A
authors.initials: RA
authors.lastname: Gonzalez-Villalobos
authors.forename: Charlotte
authors.initials: C
authors.lastname: Vaughn
authors.forename: A
authors.initials: A
authors.lastname: Michael Tabony
authors.forename: Patrice
authors.initials: P
authors.lastname: Delafontaine
mesh_terms: D000804:Angiotensin II / Q000009:adverse effects*; D000818:Animals; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000378:metabolism; D009133:Muscular Atrophy / Q000139:chemically induced* / Q000378:metabolism; D019255:NADPH Oxidases / Q000235:genetics; D010084:Oxidation-Reduction; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism; D019282:Wasting Syndrome / Q000139:chemically induced* / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D013481:Superoxides; D000804:Angiotensin II; D019255:NADPH Oxidases; C060505:neutrophil cytosolic factor 1; D046988:Proteasome Endopeptidase Complex