title: Mitochondria associate with P-bodies and modulate microRNA-mediated RNA interference.
abstract: P-bodies are cytoplasmic granules that are linked to mRNA decay, mRNA storage, and RNA interference (RNAi). They are known to interact with stress granules in stressed cells, and with late endosomes. Here, we report that P-bodies also interact with mitochondria, as previously described for P-body-related granules in germ cells. The interaction is dynamic, as a large majority of P-bodies contacts mitochondria at least once within a 3-min interval, and for about 18 s. This association requires an intact microtubule network. The depletion of P-bodies does not seem to affect mitochondria, nor the mitochondrial activity to be required for their contacts with P-bodies. However, inactivation of mitochondria leads to a strong decrease of miRNA-mediated RNAi efficiency, and to a lesser extent of siRNA-mediated RNAi. The defect occurs during the assembly of active RISC and is associated with a specific delocalization of endogeneous Ago2 from P-bodies. Our study reveals the possible involvement of RNAi defect in pathologies involving mitochondrial deficiencies.
authors.affiliation: LBPA, CNRS, Ecole Normale Supérieure de Cachan, 94230 Cachan, France.
authors.forename: Lue
authors.initials: L
authors.lastname: Huang
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Mollet
authors.forename: Sylvie
authors.initials: S
authors.lastname: Souquere
authors.forename: Florence
authors.initials: F
authors.lastname: Le Roy
authors.forename: Michèle
authors.initials: M
authors.lastname: Ernoult-Lange
authors.forename: Gérard
authors.initials: G
authors.lastname: Pierron
authors.forename: François
authors.initials: F
authors.lastname: Dautry
authors.forename: Dominique
authors.initials: D
authors.lastname: Weil
mesh_terms: D060565:Argonaute Proteins; D003594:Cytoplasmic Granules / Q000235:genetics / Q000378:metabolism*; D015852:Eukaryotic Initiation Factor-2 / Q000235:genetics / Q000378:metabolism; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D028361:Mitochondrial Diseases / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D021381:Protein Transport / Q000235:genetics; D034622:RNA Interference; D020871:RNA Stability; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*
chemical_list: C466422:AGO2 protein, human; D060565:Argonaute Proteins; D015852:Eukaryotic Initiation Factor-2; D035683:MicroRNAs; D012333:RNA, Messenger