title: Development of eight polymorphic microsatellites for a Zn/Cd hyperaccumulator Sedum alfredii Hance (Crassulaceae).
abstract: Sedum alfredii is a Zn/Cd hyperaccumulator distributed in East Asia. A total of eight polymorphic microsatellite markers were developed. These loci were screened in 25 individuals from one heavy metal-tolerant population and one nontolerant population, respectively. The average allele number of these markers was 5.25 per locus, ranging from two to nine. Population-specific alleles were found at each locus. The observed and expected heterozygosities ranged from 0.000 to 0.640 and from 0.451 to 0.819. Significant deviation from Hardy-Weinberg equilibrium was detected at both the species and the population level. No significant linkage disequilibrium was detected at population level.
authors.affiliation: South China Botanical Garden, the Chinese Academy of Sciences, Guangzhou 510650, China, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China, Graduate School of the Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Hui-Run
authors.initials: HR
authors.lastname: Huang
authors.forename: Wen-Sheng
authors.initials: WS
authors.lastname: Shu
authors.forename: Zhi-Bin
authors.initials: ZB
authors.lastname: Mao
authors.forename: Xue-Jun
authors.initials: XJ
authors.lastname: Ge