title: Isolation and characterization of polymorphic microsatellite and COI loci from the whelk Kelletia kelletii.
abstract: There is considerable interest in the genetic structure of Kelletia kelletii because of its economic, ecological and scientific importance. To that end, we developed species-specific primers which amplify mitochondrial cytochrome c oxidase subunit I as well as 13 hypervariable nuclear microsatellite loci. Using dye-labelled primers, the microsatellite loci can be co-amplified in two multiplex polymerase chain reactions and scored simultaneously on an automated sequencer.
authors.affiliation: Department of Ecology, Evolution & Marine Biology, University of California, Santa Barbara, CA 93106, USA, Hawaii Institute of Marine Biology, University of Hawaii at Manoa, Kaneohe, HI 96744, USA.
authors.forename: Crow
authors.initials: C
authors.lastname: White
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Toonen