title: Enzymatic enrichment of polyunsaturated fatty acids using novel lipase preparations modified by combination of immobilization and fish oil treatment.
abstract: Novel modification methods for lipase biocatalysts effective in hydrolysis of fish oil for enrichment of polyunsaturated fatty acids (PUFAs) were described. Based on conventional immobilization in single aqueous medium, immobilization of lipase in two phase medium composed of buffer and octane was employed. Furthermore, immobilization (in single aqueous or in two phase medium) coupled to fish oil treatment was integrated. Among these, lipase immobilized in two phase medium coupled to fish oil treatment (IMLAOF) had advantages over other modified lipases in initial reaction rate and hydrolysis degree. The hydrolysis degree increased from 12% with the free lipase to 40% with IMLAOF. Strong polar and hydrophobic solvents had negative impact on immobilization-fish oil treatment lipases, while low polar solvents were helpful to maintain the modification effect of immobilization-fish oil treatment. After five cycles of usage, the immobilization-fish oil treatment lipases still maintained more than 80% of relative hydrolysis degree.
authors.affiliation: Key Laboratory of Molecular Biophysics, Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China. yjiny@126.com
authors.forename: Jinyong
authors.initials: J
authors.lastname: Yan
authors.forename: Sanxiong
authors.initials: S
authors.lastname: Liu
authors.forename: Jiang
authors.initials: J
authors.lastname: Hu
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Gui
authors.forename: Guilong
authors.initials: G
authors.lastname: Wang
authors.forename: Yunjun
authors.initials: Y
authors.lastname: Yan
mesh_terms: D000818:Animals; D001709:Biotechnology / Q000379:methods*; D002021:Buffers; D004281:Docosahexaenoic Acids / Q000032:analysis; D015118:Eicosapentaenoic Acid / Q000032:analysis; D004795:Enzyme Stability / Q000187:drug effects; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D005395:Fish Oils / Q000494:pharmacology*; D005848:Geotrichum / Q000187:drug effects / Q000201:enzymology; D006868:Hydrolysis / Q000187:drug effects; D008049:Lipase / Q000378:metabolism*; D009795:Octanes / Q000494:pharmacology; D059027:Recycling; D012997:Solvents / Q000494:pharmacology; D013696:Temperature
chemical_list: D002021:Buffers; D004800:Enzymes, Immobilized; D005231:Fatty Acids, Unsaturated; D005395:Fish Oils; D009795:Octanes; D012997:Solvents; D004281:Docosahexaenoic Acids; D015118:Eicosapentaenoic Acid; D008049:Lipase; C026728:octane