title: [Effects of As2O3 and all-trans retinoic acid on the growth of HeLa cell line and their relation with gene NDRG1].
abstract: OBJECTIVE
  To study the effect of arsenic trioxide (As2O3) and all-trans retinoic acid (ATRA) on human cervical carcinoma HeLa cell line.
  
  
  METHODS
  HeLa cells were treated with As2O3 and ATRA. The cell proliferation was evaluated by MTT assay. The expressions of NDRG-1 protein and mRNA were determined by Western blot and RT-PCR analysis.
  
  
  RESULTS
  MTT assay showed that As2O3 and ATRA inhibited the growth of human cervical carcinoma HeLa cells in vitro in a dose- and time-dependent manner. Western blot and RT-PCR techniques showed that As2O3 and ATRA down-regulated the expressions of NDRG-1 protein and mRNA (P < 0.05).
  
  
  CONCLUSIONS
  As2O3 and ATRA can significantly inhibit the growth and proliferation of HeLa cells. The reason of these changes may be related with the down-regulation of expression of NDRG-1.
authors.affiliation: Tumor Hospital Affiliated with Harbin Medical University, Harbin 150080, China.
authors.forename: Xiao-Xing
authors.initials: XX
authors.lastname: Geng
authors.forename: Li-Na
authors.initials: LN
authors.lastname: Quan
authors.forename: Rong
authors.initials: R
authors.lastname: Ma
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Tang
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000008:administration & dosage / Q000494:pharmacology*; D015153:Blotting, Western; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D049109:Cell Proliferation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D006367:HeLa Cells; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D010087:Oxides / Q000008:administration & dosage / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014212:Tretinoin / Q000008:administration & dosage / Q000494:pharmacology*; D000099259:N-myc Downstream-Regulated Gene 1 Protein
chemical_list: D000970:Antineoplastic Agents; D000077237:Arsenic Trioxide; D001152:Arsenicals; D018797:Cell Cycle Proteins; D047908:Intracellular Signaling Peptides and Proteins; D010087:Oxides; D012333:RNA, Messenger; D014212:Tretinoin; D000099259:N-myc Downstream-Regulated Gene 1 Protein