title: Three new cytochalasins from the marine-derived fungus Spicaria elegans KLA03 by supplementing the cultures with L- and D-tryptophan.
abstract: Three new cytochalasins Z(21) -Z(23) (1-3, resp.), together with five analogs, 4-8, were isolated from Spicaria elegans KLA03 by the OSMAC (one strain-many compounds) approach with adding L- and D-tryptophan during its cultivation. The structures of new cytochalasins were elucidated on the basis of comprehensive 1D- and 2D-NMR and HR-ESI-MS analyses. Cytochalasins Z(21) and Z(22) (1 and 2, resp.), and compound 5 showed cytotoxic activities against A-549 cell lines with IC(50) values of 8.2, 20.0, and 3.1 μM, respectively.
authors.affiliation: Key Laboratory of Marine Drugs, Chinese Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao, P. R. China.
authors.forename: Fa-Zuo
authors.initials: FZ
authors.lastname: Wang
authors.forename: Hong-Juan
authors.initials: HJ
authors.lastname: Wei
authors.forename: Tian-Jiao
authors.initials: TJ
authors.lastname: Zhu
authors.forename: De-Hai
authors.initials: DH
authors.lastname: Li
authors.forename: Zhen-Jian
authors.initials: ZJ
authors.lastname: Lin
authors.forename: Qian-Qun
authors.initials: QQ
authors.lastname: Gu
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003572:Cytochalasins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D005658:Fungi / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D009369:Neoplasms / Q000188:drug therapy; D014364:Tryptophan / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D003572:Cytochalasins; C470240:cytochalasin Z; D014364:Tryptophan