title: Microsatellites from the charcoal rot fungus (Macrophomina phaseolina).
abstract: Microsatellite loci were identified from the charcoal rot fungus (Macrophomina phaseolina). Primer pairs for 46 loci were developed, and of these, 13 were optimized and screened using genomic DNA from 55 fungal isolates collected predominantly from two soybean fields in Mississippi. Twelve of the optimized loci were polymorphic and the number of alleles per locus ranged from 6 to 22. These microsatellites will be useful in population and pathogenicity studies to correspond with development of potential disease-resistant soybean and other susceptible crops.
authors.affiliation: Entomology and Plant Pathology, Mississippi State University, Starkville, MS 39762, USA, Entomology and Plant Pathology, University of Tennessee, Knoxville, TN 37996, USA, USDA-ARS Southern Horticultural Laboratory, Poplarville, MS 39470, USA, USDA-ARS-CGRU MSA, Stoneville, MS 38776, USA, University of Minnesota, Minneapolis, MN 54455 USA.
authors.forename: Richard E
authors.initials: RE
authors.lastname: Baird
authors.forename: Phillip A
authors.initials: PA
authors.lastname: Wadl
authors.forename: Xinwang
authors.initials: X
authors.lastname: Wang
authors.forename: Denita H
authors.initials: DH
authors.lastname: Johnson
authors.forename: Timothy A
authors.initials: TA
authors.lastname: Rinehart
authors.forename: Hamed K
authors.initials: HK
authors.lastname: Abbas
authors.forename: Thomas
authors.initials: T
authors.lastname: Shier
authors.forename: Robert N
authors.initials: RN
authors.lastname: Trigiano