title: Chryseobacterium vietnamense sp. nov., isolated from forest soil.
abstract: A Gram-reaction-negative bacterial strain, designated GIMN1.005T, was isolated from a forest soil sample in Vietnam. The isolate was yellow-pigmented, strictly aerobic, and unable to grow below 5 °C or above 37 °C and in the presence of more than 2.0 % NaCl. Cells were non-motile, non-gliding and non-spore-forming. The yellow pigment was of the flexirubin type, non-diffusible and non-fluorescent. Analysis of 16S rRNA gene sequences showed that strain GIMN1.005T occupied a distinct lineage within the genus Chryseobacterium, with sequence similarity values of 98.6, 98.5 and 98.3 % to Chryseobacterium aquifrigidense KCTC 12894T, Chryseobacterium jejuense KACC 12501T and Chryseobacterium indologenes GIFU 1347T, respectively. The level of DNA-DNA relatedness between strain GIMN1.005T and C. jejuense KACC 12501T was <30 %. The DNA G+C content of strain GIMN1.005T was 42.1 mol%. The predominant cellular fatty acids were iso-C15:0, iso-C17:0 3-OH and summed feature 3 (C16:1ω7c and/or C16:1ω6c); menaquinone 6 (MK-6) was the sole respiratory quinone. On the basis of phenotypic properties and phylogenetic distinctiveness, strain GIMN1.005T is considered to represent a novel species of the genus Chryseobacterium, for which the name Chryseobacterium vietnamense sp. nov. is proposed. The type strain is GIMN1.005T (=CCTCC M 209230T=NRRL B-59550T).
authors.affiliation: State Key Laboratory of Applied Microbiology (Ministry-Guangdong Province Jointly Breeding Base), South China Guangdong Provincial Microbial Culture Collection and Application Key Laboratory, Guangdong Institute of Microbiology, Guangzhou 510070, PR China.
authors.forename: Zhikun
authors.initials: Z
authors.lastname: Li
authors.affiliation: State Key Laboratory of Applied Microbiology (Ministry-Guangdong Province Jointly Breeding Base), South China Guangdong Provincial Microbial Culture Collection and Application Key Laboratory, Guangdong Institute of Microbiology, Guangzhou 510070, PR China.
authors.forename: Honghui
authors.initials: H
authors.lastname: Zhu
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D045247:Chryseobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D010858:Pigmentation; D011090:Polyenes / Q000737:chemistry; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014197:Trees / Q000382:microbiology*; D014744:Vietnam; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D011090:Polyenes; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C017313:flexirubins; C042455:menaquinone 6