title: Continuous production of isopropanol and butanol using Clostridium beijerinckii DSM 6423.
abstract: Clostridium beijerinckii DSM 6423 was studied using different continuous production methods to give maximum and stable production of isopropanol and n-butanol. In a single-stage continuous culture, when wood pulp was added as a cell holding material, we could increase the solvent productivity from 0.47 to 5.52 g L⁻¹ h⁻¹ with the yield of 54% from glucose. The overall solvent concentration of 7.51 g L⁻¹ (39.4% isopropanol and 60.6% n-butanol) with the maximum solvent productivity of 0.84 g L⁻¹ h⁻¹ was obtained with two-stage continuous culture. We were able to run the process for more than 48 overall retention times without losing the ability to produce solvents.
authors.affiliation: School of Chemical Technology, Department of Biotechnology and Chemical Technology, Aalto University, POB 16100, 00076 Aalto, Finland. shrikant.survase@aalto.fi
authors.forename: Shrikant A
authors.initials: SA
authors.lastname: Survase
authors.forename: German
authors.initials: G
authors.lastname: Jurgens
authors.forename: Adriaan
authors.initials: A
authors.lastname: van Heiningen
authors.forename: Tom
authors.initials: T
authors.lastname: Granström
mesh_terms: D019840:2-Propanol / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology; D000440:Butanols / Q000378:metabolism*; D046970:Clostridium beijerinckii / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D007218:Industrial Microbiology / Q000379:methods*
chemical_list: D000440:Butanols; D005947:Glucose; D019840:2-Propanol