title: Isolation of the Chinese rose sHSP gene promoter and its differential regulation analysis in transgenic Arabidopsis plants.
abstract: In our previous study, we identified a Rosa chinensis heat shock protein (HSP) gene, RcHSP17.8, which was induced by abiotic stresses, such as high temperature and osmotic stress. To analyze the expression of RcHSP17.8 and the function of cis-acting elements in the promoter region, a 1,910 bp fragment of the upstream sequence of the RcHSP17.8 translation initiation codon and five promoter deletion fragments were fused to a β-glucuronidase (GUS) report gene. These plasmids were transferred to Arabidopsis thaliana via Agrobacterium. GUS staining was seen in all the organs, especially in the vascular tissues after heat treatment. In transgenic Arabidopsis, GUS expression driven by the full length promoter was significantly higher under heat shock, but no GUS activity was detected under other abiotic stresses. Deletion analysis indicated that the region from -178 to -771 was essential for the promoter's response to high temperature.
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, 220 Handan Road, Shanghai 200433, People's Republic of China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Zhang
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Hu
authors.forename: Changhua
authors.initials: C
authors.lastname: Jiang
authors.forename: Wenzheng
authors.initials: W
authors.lastname: Zhang
authors.forename: Zhong
authors.initials: Z
authors.lastname: Li
authors.forename: Feng
authors.initials: F
authors.lastname: Ming
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D017931:DNA Primers / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology*; D017930:Genes, Reporter / Q000235:genetics; D005966:Glucuronidase / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D006358:Hot Temperature; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D027826:Rosa / Q000235:genetics*
chemical_list: D017931:DNA Primers; D006360:Heat-Shock Proteins; D010940:Plant Proteins; D005966:Glucuronidase