title: Dynamic expression of the main related indicators of thrombosis, inflammatory reaction and tissue damage in a rat model of myocardial infarction.
abstract: In this study, the dynamic expression of granule membrane protein-140 (GMP-140), tissue-type plasminogen activator (t-PA), high-sensitivity C-reactive protein (hs-CRP), interleukin (IL)-6, matrix metalloproteinase (MMP)-9 and tissue inhibitor of metalloproteinase (TIMP)-1 was observed in a rat model of myocardial infarction. Out of 276 Wistar rats, 6 were randomly selected as the control group, and the rest were randomly divided into 18 groups, with 15 rats in each group. The left coronary artery was ligated in 9 groups to establish the myocardial infarction model, and the remaining 9 groups served as the sham-operated groups without ligature. Of the surviving rats, 6 were randomly selected from each myocardial infarction group and sham-operated group, respectively, at 6, 12, 18, 24 and 36 h, and at 2, 3, 5 and 7 days after successful modeling. Serum levels of GMP-140, t-PA, hs-CRP, IL-6, MMP-9 and TIMP-1 were then detected using an enzyme-linked immunoassay. The results revealed that, after successful modeling, the serum levels of GMP-140 and MMP-9 continually increased, reaching the first peak at 18 h and the maximum peak on day 2. Levels then decreased slightly, but remained higher than those of the control group (p<0.05). The serum levels of hs-CRP and IL-6 increased, reached a peak at 18 h, and then decreased slightly, but were still significantly higher than those of the control group (p<0.05). The serum level of t-PA decreased significantly (p<0.05) and was restored to baseline by day 5. The serum level of TIMP-1 started to decrease as of 24 h, but was maintained until day 7 (p<0.05). In conclusion, in a rat model of myocardial infarction, the thrombosis, inflammatory reaction and tissue damage-related indicators GMP-140, hs-CRP, IL-6 and MMP-9 increased significantly, while t-PA and TIMP-1 decreased dynamically. Based on the dynamic changes of each indicator, the optimal intervention time may be determined.
authors.affiliation: Department of Cardiovascular Disease, China Academy of Chinese Medical Sciences, Beijing 100091, People Republic of China.
authors.forename: Mei
authors.initials: M
authors.lastname: Xue
authors.forename: Huijun
authors.initials: H
authors.lastname: Yin
authors.forename: Lu
authors.initials: L
authors.lastname: Zhang
authors.forename: Chunyu
authors.initials: C
authors.lastname: Guo
authors.forename: Yuerong
authors.initials: Y
authors.lastname: Jiang
authors.forename: Caifeng
authors.initials: C
authors.lastname: Wu
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Li
authors.forename: Keji
authors.initials: K
authors.lastname: Chen
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis / Q000097:blood*; D002097:C-Reactive Protein / Q000032:analysis; D004195:Disease Models, Animal; D007249:Inflammation / Q000150:complications; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000097:blood; D009203:Myocardial Infarction / Q000209:etiology / Q000378:metabolism*; D019007:P-Selectin / Q000097:blood; D051381:Rats; D017208:Rats, Wistar; D013927:Thrombosis / Q000150:complications; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000097:blood; D010959:Tissue Plasminogen Activator / Q000097:blood
chemical_list: D015415:Biomarkers; D015850:Interleukin-6; D019007:P-Selectin; D019715:Tissue Inhibitor of Metalloproteinase-1; D002097:C-Reactive Protein; D010959:Tissue Plasminogen Activator; D020780:Matrix Metalloproteinase 9