title: Toxin-binding proteins isolated from yellow mealworm Tenebrio molitor and wax moth Galleria mellonella.
abstract: A 67-kDa protein that can specifically bind the activated Cry9A endotoxin under ligand-blotting conditions was purified from midgut epithelium apical membranes of wax moth Galleria mellonella by affinity chromatography. N-Terminal amino acid sequencing enabled identification of this protein as aminopeptidase N. In similar experiments, 66- and 58-kDa proteins specific to endotoxin Cry3A were isolated from the midgut epithelium apical membranes of Tenebrio molitor larvae. Mass spectrometry showed close similarity of the 58-kDa protein to the Tenebrio molitor α-amylase.
authors.affiliation: Scientific Research Institute for Genetics and Selection of Industrial Microorganisms, Moscow, Russia. nat86@yandex.ru
authors.forename: N V
authors.initials: NV
authors.lastname: Bulushova
authors.forename: D P
authors.initials: DP
authors.lastname: Zhuzhikov
authors.forename: L I
authors.initials: LI
authors.lastname: Lyutikova
authors.forename: N E
authors.initials: NE
authors.lastname: Kirillova
authors.forename: I A
authors.initials: IA
authors.lastname: Zalunin
authors.forename: G G
authors.initials: GG
authors.lastname: Chestukhina
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001413:Bacillus thuringiensis; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D018826:CD13 Antigens / Q000737:chemistry / Q000302:isolation & purification; D004064:Digestive System / Q000378:metabolism; D004731:Endotoxins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D006460:Hemolysin Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D019476:Insect Proteins / Q000737:chemistry / Q000302:isolation & purification*; D007814:Larva / Q000201:enzymology; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009036:Moths / Q000201:enzymology; D011485:Protein Binding; D013712:Tenebrio / Q000201:enzymology; D000516:alpha-Amylases / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; D019476:Insect Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis; D000516:alpha-Amylases; D018826:CD13 Antigens