title: Analysis for complete genomic sequence of HLA-B and HLA-C alleles in the Chinese Han population.
abstract: In the present study, we have determined the complete genomic sequence and analysed the intron polymorphism of partial HLA-B and HLA-C alleles in the Chinese Han population. Over 3.0 kb DNA fragments of HLA-B and HLA-C loci were amplified by polymerase chain reaction from partial 5' untranslated region to 3' noncoding region respectively, and then the amplified products were sequenced. Full-length nucleotide sequences of 14 HLA-B alleles and 10 HLA-C alleles were obtained and have been submitted to GenBank and IMGT/HLA database. Two novel alleles of HLA-B*52:01:01:02 and HLA-B*59:01:01:02 were identified, and the complete genomic sequence of HLA-B*52:01:01:01 was firstly reported. Totally 157 and 167 polymorphism positions were found in the full-length genomic sequence of HLA-B and HLA-C loci respectively. Our results suggested that many single nucleotide polymorphisms existed in the exon and intron regions, and the data can provide useful information for understanding the evolution of HLA-B and HLA-C alleles.
authors.affiliation: HLA Typing Laboratory, Blood Center of Zhejiang Province and Key Laboratory of Blood Safety Research, Ministry of Health, Hangzhou, Zhejiang Province, China.
authors.forename: F
authors.initials: F
authors.lastname: Zhu
authors.forename: Y
authors.initials: Y
authors.lastname: He
authors.forename: W
authors.initials: W
authors.lastname: Zhang
authors.forename: J
authors.initials: J
authors.lastname: He
authors.forename: J
authors.initials: J
authors.lastname: He
authors.forename: X
authors.initials: X
authors.lastname: Xu
authors.forename: H
authors.initials: H
authors.lastname: Lv
authors.forename: L
authors.initials: L
authors.lastname: Yan
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D020121:5' Untranslated Regions / Q000235:genetics; D000483:Alleles; D044466:Asian People / Q000235:genetics*; D019143:Evolution, Molecular; D005091:Exons; D016678:Genome / Q000235:genetics*; D015235:HLA-B Antigens / Q000235:genetics*; D015236:HLA-C Antigens / Q000235:genetics*; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic
chemical_list: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D015235:HLA-B Antigens; D015236:HLA-C Antigens