title: Specific Silencing of L392V PSEN1 Mutant Allele by RNA Interference.
abstract: RNA interference (RNAi) technology provides a powerful molecular tool to reduce an expression of selected genes in eukaryotic cells. Short interfering RNAs (siRNAs) are the effector molecules that trigger RNAi. Here, we describe siRNAs that discriminate between the wild type and mutant (1174 C→G) alleles of human Presenilin1 gene (PSEN1). This mutation, resulting in L392V PSEN1 variant, contributes to early onset familial Alzheimer's disease. Using the dual fluorescence assay, flow cytometry and fluorescent microscopy we identified positions 8th-11th, within the central part of the antisense strand, as the most sensitive to mismatches. 2-Thiouridine chemical modification introduced at the 3'-end of the antisense strand improved the allele discrimination, but wobble base pairing adjacent to the mutation site abolished the siRNA activity. Our data indicate that siRNAs can be designed to discriminate between the wild type and mutant alleles of genes that differ by just a single nucleotide.
authors.affiliation: Department of Bioorganic Chemistry, Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, 90-363 Lodz, Sienkiewicza 112, Poland.
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Sierant
authors.forename: Alina
authors.initials: A
authors.lastname: Paduszynska
authors.forename: Julia
authors.initials: J
authors.lastname: Kazmierczak-Baranska
authors.forename: Benedetta
authors.initials: B
authors.lastname: Nacmias
authors.forename: Sandro
authors.initials: S
authors.lastname: Sorbi
authors.forename: Silvia
authors.initials: S
authors.lastname: Bagnoli
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Sochacka
authors.forename: Barbara
authors.initials: B
authors.lastname: Nawrot