title: Analysis of intracellular distribution of Borna disease virus glycoprotein fused with fluorescent markers in living cells.
abstract: Borna disease virus (BDV) is a non-segmented, negative-strand RNA virus that is characterized by nuclear replication and persistent infection. A unique feature of BDV is that it releases only a small number of infectious particles from infected cells. Although these characteristics might make it difficult to obtain a large amount of recombinant viruses in a reverse genetics system, the mechanism underlying the budding or assembly of BDV particle has remained largely unknown. In this study, as a first step toward understanding the virion formation of BDV, we investigated the intracellular distribution and mobility of the fluorescent marker fusion envelope glycoprotein (G) of BDV in living cells. Expression analysis revealed that fusion proteins seem to cleave into functional subunits and localize in the endoplasmic reticulum (ER)/Golgi apparatus, as well as the authentic BDV G. Furthermore, we demonstrated using fluorescence recovery after photobleaching analysis that BDV G fluorescence shows rapid recovery in both the ER/Golgi and plasma membrane regions, indicating that BDV G fusion protein may be a useful tool to investigate not only the maturation of BDV G but also the budding and assembly of BDV particles in living cells.
authors.affiliation: Department of Virology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565–0871, Japan.
authors.forename: Takuji
authors.initials: T
authors.lastname: Daito
authors.forename: Kan
authors.initials: K
authors.lastname: Fujino
authors.forename: Yohei
authors.initials: Y
authors.lastname: Watanabe
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Ikuta
authors.forename: Keizo
authors.initials: K
authors.lastname: Tomonaga
mesh_terms: D000818:Animals; D001891:Borna disease virus / Q000502:physiology*; D002462:Cell Membrane; D002522:Chlorocebus aethiops; D004721:Endoplasmic Reticulum; D005453:Fluorescence; D015967:Gene Expression Regulation, Viral / Q000502:physiology*; D006056:Golgi Apparatus; D008164:Luminescent Proteins / Q000378:metabolism*; D014709:Vero Cells; D014760:Viral Fusion Proteins / Q000235:genetics / Q000378:metabolism*; D000097573:Red Fluorescent Protein
chemical_list: D008164:Luminescent Proteins; D014760:Viral Fusion Proteins; D000097573:Red Fluorescent Protein