title: The Prp19 complex is a novel transcription elongation factor required for TREX occupancy at transcribed genes.
abstract: Different steps in gene expression are intimately linked. In Saccharomyces cerevisiae, the conserved TREX complex couples transcription to nuclear messenger RNA (mRNA) export. However, it is unknown how TREX is recruited to actively transcribed genes. Here, we show that the Prp19 splicing complex functions in transcription elongation. The Prp19 complex is recruited to transcribed genes, interacts with RNA polymerase II (RNAPII) and TREX, and is absolutely required for TREX occupancy at transcribed genes. Importantly, the Prp19 complex is necessary for full transcriptional activity. Taken together, we identify the Prp19 splicing complex as a novel transcription elongation factor that is essential for TREX occupancy at transcribed genes and that thus provides a novel link between transcription and messenger ribonucleoprotein (mRNP) formation.
authors.affiliation: Gene Center Munich, Department of Biochemistry, Ludwig-Maximilians-University Munich, Germany.
authors.forename: Sittinan
authors.initials: S
authors.lastname: Chanarat
authors.forename: Martin
authors.initials: M
authors.lastname: Seizl
authors.forename: Katja
authors.initials: K
authors.lastname: Strässer
mesh_terms: D000963:Antimetabolites / Q000494:pharmacology; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D025141:Drug Resistance, Fungal / Q000235:genetics; D000072260:RNA Splicing Factors; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D017384:Sequence Deletion; D017381:Spliceosomes / Q000235:genetics / Q000378:metabolism*; D035602:Transcriptional Elongation Factors / Q000378:metabolism*; D014498:Uracil / Q000031:analogs & derivatives / Q000494:pharmacology
chemical_list: D000963:Antimetabolites; D018797:Cell Cycle Proteins; C079877:PRP19 protein, S cerevisiae; D000072260:RNA Splicing Factors; D029701:Saccharomyces cerevisiae Proteins; C419764:Syf1 protein, S cerevisiae; D035602:Transcriptional Elongation Factors; D014498:Uracil; C100248:azauracil