title: [Induction of monocyte-derived dendritic cell differentiation by asthmatic serum in a transendothelial trafficking model].
abstract: OBJECTIVE
  To explore the effect of asthmatic and healthy serum on differentiation and function of monocyte-derived dendritic cells (MDDC) in a transendothelial trafficking model.
  
  
  METHODS
  The sera and peripheral blood mononuclear cells (PBMC) were separated from 12 asthmatic patients and 12 healthy volunteers, and monocytes were selected from PBMC using magnetic beads. The trypsin-digested human umbilical vein endothelial cells (HUVEC) at passage 2 from 5 healthy lying-in women were used to construct the transendothelial trafficking model under asthmatic or healthy serum, wherein MDDC were identified by silver nitrate staining and scanning electron microscopy. Nuclear factor κB (NF-κB) activity was determined by electrophoretic mobility shift assay. Flow cytometry, ELISA and mixed leukocyte reaction were relevantly utilized to detect the phenotype, cytokine and T cell proliferation.
  
  
  RESULTS
  (1) Monocytes traversed through HUVEC monolayer after 2 h, and reverse-transmigrated to develop into DC 48 h later. (2) The healthy serum stimulated monocytes into immature MDDC with lower CD(14) [(20 ± 5)%] (F = 49.01, P < 0.05), and higher HLA-DR, CD(80), CD(86) and CD(83) [(43 ± 4)%, (17.9 ± 3.5)%, (43 ± 11)% and (6.7 ± 1.8)%, respectively] (F = 10.35 - 40.17, all P < 0.05) than monocytes did before transmigration at 0 h [CD(14) (81 ± 6)%, HLA-DR (24 ± 5)%, CD(80) (2.8 ± 2.0)%, CD(86) (14 ± 4)% and CD(83) (0.9 ± 0.8)%, respectively]. (3) The asthmatic serum stimulated monocytes into mature MDDC, characteristic of dendrites, with similar HLA-DR and CD(86) [(55 ± 6)% and (59 ± 12)%] (F = 15.29 and 35.97, all P > 0.05), higher CD(80) and CD(83) [(49.7 ± 10.2)% and (30.2 ± 6.8)%] (F = 4.01 and 20.68, all P < 0.05), accompanied by increased levels of NF-κB activity, IL-12 p70 and T cell proliferation [(100 ± 11)%, (568 ± 43) ng/L and (2033 ± 198) cpm, respectively] (F = 49.23 - 350.84, all P < 0.05) relative to the healthy serum-stimulated immature MDDC [(12 ± 3)%, (220 ± 35) ng/L and (952 ± 64) cpm, respectively].
  
  
  CONCLUSIONS
  The asthmatic serum induces mature MDDC in association with NF-κB overactivation in the transendothelial trafficking model, which provides a promising experimental platform for both investigation of immunological mechanisms in asthma and screening of novel anti-asthma drugs in vitro.
authors.affiliation: Department of Respiratory Medicine, the First Affiliated Hospital, Nanjing Medical University, China. lfzhou@njmu.edu.cn
authors.forename: Lin-fu
authors.initials: LF
authors.lastname: Zhou
authors.forename: Wen-lu
authors.initials: WL
authors.lastname: Wang
authors.forename: Hong-yan
authors.initials: HY
authors.lastname: Li
authors.forename: Ming-shun
authors.initials: MS
authors.lastname: Zhang
authors.forename: Xiao-hui
authors.initials: XH
authors.lastname: Ji
authors.forename: Shao-heng
authors.initials: SH
authors.lastname: He
authors.forename: Mao
authors.initials: M
authors.lastname: Huang
authors.forename: Kai-sheng
authors.initials: KS
authors.lastname: Yin
mesh_terms: D000293:Adolescent; D000328:Adult; D001249:Asthma / Q000097:blood*; D016022:Case-Control Studies; D002454:Cell Differentiation; D003713:Dendritic Cells / Q000166:cytology*; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology*; D008297:Male; D016328:NF-kappa B / Q000378:metabolism; D055815:Young Adult
chemical_list: D016328:NF-kappa B