title: New proteomic developments to analyze protein isomerization and their biological significance in plants.
abstract: Spontaneous isoaspartyl formation from aspartyl dehydration or asparaginyl deamidation is a major source of modifications in protein structures. In cells, these conformational changes could be reverted by the protein L-isoaspartyl methyltransferase (PIMT) repair enzyme that converts the isoaspartyl residues into aspartyl. The physiological importance of this metabolism has been recently illustrated in plants. Recent developments allowing peptide isomer identification and quantification at the proteome scale are portrayed. The relevance of these new proteomic approaches based on 2-D electrophoresis or electron capture dissociation analysis methods was initially documented in mammals. Extended use to Arabidopsis model systems is promising for the discovery of controlling mechanisms induced by these particular post-translational modifications and their biological role in plants.
authors.affiliation: Institut Jean-Pierre Bourgin, UMR1318 INRA-AgroParisTech, Institut National de la Recherche Agronomique, F-78026 Versailles cedex, France. grappin@versailles.inra.fr
authors.forename: Philippe
authors.initials: P
authors.lastname: Grappin
authors.forename: Boris
authors.initials: B
authors.lastname: Collet
authors.forename: Hongqian
authors.initials: H
authors.lastname: Yang
authors.forename: Denis
authors.initials: D
authors.lastname: Jallet
authors.forename: Laurent
authors.initials: L
authors.lastname: Ogé
authors.forename: Roman
authors.initials: R
authors.lastname: Zubarev
mesh_terms: D017360:Arabidopsis / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D026581:Isoaspartic Acid / Q000378:metabolism; D013058:Mass Spectrometry; D008745:Methylation; D019151:Peptide Library; D010944:Plants / Q000378:metabolism*; D026601:Protein D-Aspartate-L-Isoaspartate Methyltransferase / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D026581:Isoaspartic Acid; D019151:Peptide Library; D026601:Protein D-Aspartate-L-Isoaspartate Methyltransferase