title: Modeling initiation of Ewing sarcoma in human neural crest cells.
abstract: Ewing sarcoma family tumors (ESFT) are aggressive bone and soft tissue tumors that express EWS-ETS fusion genes as driver mutations. Although the histogenesis of ESFT is controversial, mesenchymal (MSC) and/or neural crest (NCSC) stem cells have been implicated as cells of origin. For the current study we evaluated the consequences of EWS-FLI1 expression in human embryonic stem cell-derived NCSC (hNCSC). Ectopic expression of EWS-FLI1 in undifferentiated hNCSC and their neuro-mesenchymal stem cell (hNC-MSC) progeny was readily tolerated and led to altered expression of both well established as well as novel EWS-FLI1 target genes. Importantly, whole genome expression profiling studies revealed that the molecular signature of established ESFT is more similar to hNCSC than any other normal tissue, including MSC, indicating that maintenance or reactivation of the NCSC program is a feature of ESFT pathogenesis. Consistent with this hypothesis, EWS-FLI1 induced hNCSC genes as well as the polycomb proteins BMI-1 and EZH2 in hNC-MSC. In addition, up-regulation of BMI-1 was associated with avoidance of cellular senescence and reversible silencing of p16. Together these studies confirm that, unlike terminally differentiated cells but consistent with bone marrow-derived MSC, NCSC tolerate expression of EWS-FLI1 and ectopic expression of the oncogene initiates transition to an ESFT-like state. In addition, to our knowledge this is the first demonstration that EWS-FLI1-mediated induction of BMI-1 and epigenetic silencing of p16 might be critical early initiating events in ESFT tumorigenesis.
authors.affiliation: Department of Pediatrics, University of Michigan, Ann Arbor, Michigan, United States of America.
authors.forename: Cornelia
authors.initials: C
authors.lastname: von Levetzow
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Jiang
authors.forename: Ynnez
authors.initials: Y
authors.lastname: Gwye
authors.forename: Gregor
authors.initials: G
authors.lastname: von Levetzow
authors.forename: Long
authors.initials: L
authors.lastname: Hung
authors.forename: Aaron
authors.initials: A
authors.lastname: Cooper
authors.forename: Jessie Hao-Ru
authors.initials: JH
authors.lastname: Hsu
authors.forename: Elizabeth R
authors.initials: ER
authors.lastname: Lawlor
mesh_terms: D015153:Blotting, Western; D002454:Cell Differentiation; D016922:Cellular Senescence; D053595:Embryonic Stem Cells / Q000166:cytology*; D044127:Epigenesis, Genetic; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D006801:Humans; D016086:Lentivirus / Q000378:metabolism; D008957:Models, Genetic; D009432:Neural Crest / Q000166:cytology*; D058953:Neural Stem Cells / Q000166:cytology; D009857:Oncogenes; D012512:Sarcoma, Ewing / Q000175:diagnosis*