title: Expression and prognostic value of WISP-1 in patients with endometrial endometrioid adenocarcinoma.
abstract: OBJECTIVE
  It has been well established that the aberrant activation of the Wnt/β-catenin signal correlates to endometrial endometrioid adenocarcinoma (EEC). As an effector of the Wnt/β-catenin mediated antiapoptotic signal, the role of Wnt-induced secreted protein-1 (WISP-1) in EEC still remains unclear.
  
  
  METHODS
  We used immunohistochemistry and quantitative real-time RT-PCR to examine the expression of WISP-1, the estrogen receptor (ER) and progesterone receptor (PR) in 86 cases of EEC, with 20 cases of endometrial hyperplasia, 20 of proliferative endometrium and 20 of secretory endometrium used as the control group. We also correlated the expression of WISP-1 with various clinicopathologic factors and prognostic values in patients with EEC.
  
  
  RESULTS
  A high expression of WISP-1 was observed in 26 of the 86 cases of EEC (30.2%). The expression rate of WISP-1 in EEC was significantly higher than that in secretory endometrium (P < 0.005). Histopathological grades and PR were associated with high WISP-1 expression (P = 0.002, P = 0.027, respectively). The estimated five-year survival rate of patients with low-to-moderate expression of WISP-1 was significantly higher than those with high WISP-1 expression (91.3% vs 65%, P = 0.011). Multivariate analysis revealed that high WISP-1 expression and positive lymphovascular space invasion were independent prognostic factors for survival.
  
  
  CONCLUSIONS
  High expression of WISP-1 was related to tumor cell dedifferentiation and PR loss. WISP-1 might be a new molecular marker to predict the prognosis of patients with EEC.
authors.affiliation: Department of Pathology, The Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China.
authors.forename: Qionglan
authors.initials: Q
authors.lastname: Tang
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Jiang
authors.forename: Haigang
authors.initials: H
authors.lastname: Li
authors.forename: Zhongqiu
authors.initials: Z
authors.lastname: Lin
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Zhou
authors.forename: Xin
authors.initials: X
authors.lastname: Luo
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.forename: Gengbiao
authors.initials: G
authors.lastname: Chen
mesh_terms: D000328:Adult; D000368:Aged; D055511:CCN Intercellular Signaling Proteins / Q000235:genetics / Q000378:metabolism*; D018269:Carcinoma, Endometrioid / Q000175:diagnosis* / Q000378:metabolism* / Q000473:pathology; D015331:Cohort Studies; D016889:Endometrial Neoplasms / Q000175:diagnosis* / Q000378:metabolism* / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008875:Middle Aged; D011379:Prognosis; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D016019:Survival Analysis
chemical_list: D055511:CCN Intercellular Signaling Proteins; C116396:CCN4 protein, human; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger