title: PERMANENT GENETIC RESOURCES: A set of 12 microsatellite loci for genetic studies of Leishmania braziliensis.
abstract: Twelve microsatellite loci of Leishmania braziliensis were examined, nine of which were developed in this work. Fifty-six Leishmania braziliensis were genotyped with these microsatellite loci. The 12 loci studied were polymorphic with the number of alleles ranging from five to 19, with a mean of 9.7 ± 4.1 and the observed heterozygosity averaging 0.425 ± 0.202. The important heterozygote deficits we observed (F(IS)  = 0.41, P value = 0.004) appear incompatible with the heterozygote excess expected in clonal diploids. This last result could revive the clonality/sexuality debate regarding Leishmania. This work validates the potential use of these microsatellites for population genetics analysis.
authors.affiliation: Genetique et Evolution des Maladies Infectieuses, IRD/CNRS (UMR2724), Centre, IRD, 911, Avenue Agropolis BP 64501, F-34394, Montpellier Cedex 5, France, Instituto de Medicina Tropical 'Alexander von Humboldt', Lima, Peru.
authors.forename: V
authors.initials: V
authors.lastname: Rougeron
authors.forename: E
authors.initials: E
authors.lastname: Waleckx
authors.forename: M
authors.initials: M
authors.lastname: Hide
authors.forename: T
authors.initials: T
authors.lastname: DE Meeûs
authors.forename: J
authors.initials: J
authors.lastname: Arevalo
authors.forename: A
authors.initials: A
authors.lastname: Llanos-Cuentas
authors.forename: A L
authors.initials: AL
authors.lastname: Bañuls