title: Development of novel microsatellites from Moniliophthora perniciosa, causal agent of the witches' broom disease of Theobroma cacao.
abstract: Moniliophthora perniciosa is the causal agent of the witches' broom disease of cacao. Based on available genomic sequences, we identified 30 new microsatellite loci, which were analysed using 50 isolates from four populations sampled over a wide geographical area in Brazil, including three populations from the Amazon, the fungal putative centre of diversity, plus one from Bahia. Nine loci were polymorphic, with an average of 2.9 alleles per locus. The level of polymorphism observed was low, but these markers may allow the evaluation of pathogen diversity and the establishment of molecular standards for isolate fingerprinting to support cacao breeding.
authors.affiliation: Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, CP 96, Piracicaba, SP 13400-970, Brazil, Universidade Estadual de Campinas, CP 6109, Campinas, SP 13083-970, Brazil, Comissão Executiva do Plano da Lavoura Cacaueira, CP 46, Belém, PA 66000-000, Brazil.
authors.forename: Jurema R Q
authors.initials: JR
authors.lastname: Silva
authors.forename: Antonio
authors.initials: A
authors.lastname: Figueira
authors.forename: Gonçalo A G
authors.initials: GA
authors.lastname: Pereira
authors.forename: Paulo
authors.initials: P
authors.lastname: Albuquerque