title: Reduced tumorigenicity and cell motility of a gastric-carcinoma cell-line by introduction of wild-type p53 gene.
abstract: Wild-type or mutant human p53 gene was transfected into a human gastric carcinoma cell line MKN-1 which shares a mutant p53 allele. Transfected wild-type p53 reduced the colony forming efficiency and tumorigenicity of MKN-1 cells. However, no difference in expression of cell adhesion molecule, oncogenes and growth factors was observed among parent, wild-type p53 and mutant p53 transfectants. In motility assay, the wild-type p53 transfectants relative to the parental or mutant p53 transfectants exhibited a decreased motility, and HGF had a greater effect on the motility of the mutant p53 transfectants, but very little effect on the motility of either the parental or wild-type transfectants. In invasion assay, mutant p53 transfectants revealed the increased invasion ability into collagen gel. These results suggest that allele loss and point mutation of p53 gene may play a critical role not only in growth but also in invasion of gastric carcinoma cells.
authors.affiliation: HIROSHIMA UNIV,SCH MED,DEPT PATHOL 1,1-2-3 KASUMI,MINAMI KU,HIROSHIMA 734,JAPAN. HIROSHIMA UNIV,NUCL BIOL & MED RES INST,DEPT PATHOL,MINAMI KU,HIROSHIMA 734,JAPAN. UNIV TEXAS,MD ANDERSON CANC CTR,DEPT NEUROONCOL,HOUSTON,TX 77030.
authors.forename: E
authors.initials: E
authors.lastname: Kyo
authors.forename: H
authors.initials: H
authors.lastname: Yokozaki
authors.forename: K
authors.initials: K
authors.lastname: Yanagihara
authors.forename: W
authors.initials: W
authors.lastname: Yasui
authors.forename: M
authors.initials: M
authors.lastname: Ito
authors.forename: T
authors.initials: T
authors.lastname: Sano
authors.forename: P
authors.initials: P
authors.lastname: Lee
authors.forename: H
authors.initials: H
authors.lastname: Saya
authors.forename: E
authors.initials: E
authors.lastname: Tahara