title: Atypical cristae morphology of human syncytiotrophoblast mitochondria: role for complex V.
abstract: Mitochondrial complexes I, III(2), and IV from human cytotrophoblast and syncytiotrophoblast associate to form supercomplexes or respirasomes, with the following stoichiometries: I(1):(III(2))(1) and I(1):(III(2))(1-2):IV(1-4). The content of respirasomes was similar in both cell types after isolating mitochondria. However, syncytiotrophoblast mitochondria possess low levels of dimeric complex V and do not have orthodox cristae morphology. In contrast, cytotrophoblast mitochondria show normal cristae morphology and a higher content of ATP synthase dimer. Consistent with the dimerizing role of the ATPase inhibitory protein (IF(1)) (García, J. J., Morales-Ríos, E., Cortés-Hernandez, P., and Rodríguez-Zavala, J. S. (2006) Biochemistry 45, 12695-12703), higher relative amounts of IF(1) were observed in cytotrophoblast when compared with syncytiotrophoblast mitochondria. Therefore, there is a correlation between dimerization of complex V, IF(1) expression, and the morphology of mitochondrial cristae in human placental mitochondria. The possible relationship between cristae architecture and the physiological function of the syncytiotrophoblast mitochondria is discussed.
authors.affiliation: Department of Biochemistry and Molecular Biology, Medicine Faculty, National Autonomous University of Mexico, 04510 Mexico City, Mexico.
authors.forename: Daniela
authors.initials: D
authors.lastname: De los Rios Castillo
authors.forename: Mariel
authors.initials: M
authors.lastname: Zarco-Zavala
authors.forename: Sofia
authors.initials: S
authors.lastname: Olvera-Sanchez
authors.forename: Juan Pablo
authors.initials: JP
authors.lastname: Pardo
authors.forename: Oscar
authors.initials: O
authors.lastname: Juarez
authors.forename: Federico
authors.initials: F
authors.lastname: Martinez
authors.forename: Guillermo
authors.initials: G
authors.lastname: Mendoza-Hernandez
authors.forename: José J
authors.initials: JJ
authors.lastname: García-Trejo
authors.forename: Oscar
authors.initials: O
authors.lastname: Flores-Herrera
mesh_terms: D006801:Humans; D008928:Mitochondria / Q000201:enzymology* / Q000648:ultrastructure*; D024101:Mitochondrial Proteins / Q000737:chemistry / Q000378:metabolism*; D009097:Multienzyme Complexes / Q000737:chemistry / Q000378:metabolism*; D014327:Trophoblasts / Q000201:enzymology* / Q000648:ultrastructure*
chemical_list: D024101:Mitochondrial Proteins; D009097:Multienzyme Complexes