title: Process evaluations and economic analyses of recombinant human lysozyme and hen egg-white lysozyme purifications.
abstract: Human lysozyme and hen egg-white lysozyme have antibacterial, antiviral, and antifungal properties with numerous potential commercial applications. Currently, hen egg-white lysozyme dominates low cost applications but the recent high-level expression of human lysozyme in rice could provide an economical source of lysozyme. This work compares human lysozyme and hen egg-white lysozyme adsorption to the cation exchange resin, SP-Sepharose FF, and the effect of rice extract components on lysozyme purification. With one exception, the dynamic binding capacities of human lysozyme were lower than those of hen egg-white at pH 4.5, 6, and 7.5 with ionic strengths ranging from 0 to 100 mM (5-20 mS). Ionic strength and pH had a similar effect on the adsorption capacities, but human lysozyme was more sensitive to these two factors than hen egg-white lysozyme. In the presence of rice extract, the dynamic binding capacities of human and hen egg-white lysozymes were reduced by 20-30% and by 32-39% at pH 6. Hen egg-white lysozyme was used as a benchmark to compare the effectiveness of human lysozyme purification from transgenic rice extract. Process simulation and cost analyses for human lysozyme purification from rice and hen egg-white lysozyme purification from egg-white resulted in similar unit production costs at 1 ton per year scale.
authors.affiliation: Dept. of Biological & Agricultural Engineering, Texas A&M University, College Station, TX 77843, USA.
authors.forename: Lisa R
authors.initials: LR
authors.lastname: Wilken
authors.forename: Zivko L
authors.initials: ZL
authors.lastname: Nikolov
mesh_terms: D000327:Adsorption; D000818:Animals; D002411:Cation Exchange Resins; D003365:Costs and Cost Analysis; D006801:Humans; D009113:Muramidase / Q000191:economics / Q000302:isolation & purification*; D012275:Oryza / Q000235:genetics; D011485:Protein Binding; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000191:economics / Q000302:isolation & purification*
chemical_list: D002411:Cation Exchange Resins; D011994:Recombinant Proteins; C421864:hen egg lysozyme; D009113:Muramidase