title: Dry-reagent gold nanoparticle-based lateral flow biosensor for the simultaneous detection of Vibrio cholerae serogroups O1 and O139.
abstract: Cholera is a communicable disease caused by consumption of contaminated food and water. This potentially fatal intestinal infection is characterised by profuse secretion of rice watery stool that can rapidly lead to severe dehydration and shock, thus requiring treatment to be given immediately. Epidemic and pandemic cholera are exclusively associated with Vibrio cholerae serogroups O1 and O139. In light of the need for rapid diagnosis of cholera and to prevent spread of outbreaks, we have developed and evaluated a direct one-step lateral flow biosensor for the simultaneous detection of both V. cholerae O1 and O139 serogroups using alkaline peptone water culture. Serogroup specific monoclonal antibodies raised against lipopolysaccharides (LPS) were used to functionalize the colloidal gold nanoparticles for dual detection in the biosensor. The assay is based on immunochromatographic principle where antigen-antibody reaction would result in the accumulation of gold nanoparticles and thus, the appearance of a red line on the strip. The dry-reagent dipstick format of the biosensor ensure user-friendly application, rapid result that can be read with the naked eyes and cold-chain free storage that is well-suited to be performed at resource-limited settings.
authors.affiliation: Department of Medical Microbiology and Parasitology, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.
authors.forename: Choo Yee
authors.initials: CY
authors.lastname: Yu
authors.forename: Geik Yong
authors.initials: GY
authors.lastname: Ang
authors.forename: Ang Lim
authors.initials: AL
authors.lastname: Chua
authors.forename: Elina Husni
authors.initials: EH
authors.lastname: Tan
authors.forename: Su Yin
authors.initials: SY
authors.lastname: Lee
authors.forename: Gustavo
authors.initials: G
authors.lastname: Falero-Diaz
authors.forename: Oscar
authors.initials: O
authors.lastname: Otero
authors.forename: Irelio
authors.initials: I
authors.lastname: Rodríguez
authors.forename: Fátima
authors.initials: F
authors.lastname: Reyes
authors.forename: Armando
authors.initials: A
authors.lastname: Acosta
authors.forename: María E
authors.initials: ME
authors.lastname: Sarmiento
authors.forename: Santanu
authors.initials: S
authors.lastname: Ghosh
authors.forename: Thandavarayan
authors.initials: T
authors.lastname: Ramamurthy
authors.forename: Chan
authors.initials: C
authors.lastname: Yean Yean
authors.forename: Pathabhiraman
authors.initials: P
authors.lastname: Lalitha
authors.forename: Manickam
authors.initials: M
authors.lastname: Ravichandran
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D000937:Antigen-Antibody Reactions; D015374:Biosensing Techniques / Q000379:methods*; D002771:Cholera / Q000175:diagnosis* / Q000276:immunology; D006046:Gold / Q000737:chemistry*; D006801:Humans; D008070:Lipopolysaccharides / Q000276:immunology; D053768:Metal Nanoparticles / Q000737:chemistry; D012680:Sensitivity and Specificity; D012698:Serologic Tests; D012703:Serotyping; D041241:Vibrio cholerae O1 / Q000276:immunology / Q000302:isolation & purification*; D041242:Vibrio cholerae O139 / Q000276:immunology / Q000302:isolation & purification*
chemical_list: D000911:Antibodies, Monoclonal; D008070:Lipopolysaccharides; D006046:Gold