title: Whooping cough in Pakistan: Bordetella pertussis vs Bordetella parapertussis in 2005-2009.
abstract: Pertussis, or whooping cough, is an acute respiratory disease mainly affecting infants and children and is caused by Bordetella pertussis and Bordetella parapertussis. The aim of this study was to investigate the share of Bordetella species from potential whooping cough cases during 2005-2009. Eight hundred and two samples from suspected pertussis cases were collected, mainly from 2 provinces of Pakistan. Bacterial culture, identification, DNA extraction and routinely used polymerase chain reaction (PCR) methods using IS1001, IS1002 and IS481 were used to identify the Bordetella species. The results were unexpected, because all of the isolates collected from the different cities were identified as B. parapertussis (7.4%); B. pertussis was not isolated from any sample. However, PCR results indicated the presence of a small percentage (0.6%) of B. pertussis among the total cases studied. This study suggests that vaccines to protect against both B. pertussis and B. parapertussis should be considered.
authors.affiliation: Department of Biosciences, COMSATS Institute of Information Technology, Islamabad, Pakistan. habib@comsats.edu.pk
authors.forename: Habib
authors.initials: H
authors.lastname: Bokhari
authors.forename: Fahad
authors.initials: F
authors.lastname: Said
authors.forename: Muhammad A
authors.initials: MA
authors.lastname: Syed
authors.forename: Amjad
authors.initials: A
authors.lastname: Mughal
authors.forename: Yasmeen F
authors.initials: YF
authors.lastname: Kazi
authors.forename: Kees
authors.initials: K
authors.lastname: Heuvelman
authors.forename: Frits R
authors.initials: FR
authors.lastname: Mooi
mesh_terms: D042483:Bordetella parapertussis / Q000235:genetics / Q000302:isolation & purification*; D001886:Bordetella pertussis / Q000235:genetics / Q000302:isolation & purification*; D015331:Cohort Studies; D004587:Electrophoresis, Agar Gel; D006801:Humans; D010154:Pakistan / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D014917:Whooping Cough / Q000453:epidemiology / Q000382:microbiology*