title: Silicate and borate glasses as composite fillers: a bioactivity and biocompatibility study.
abstract: Composites filled with a silicate glass (CSi) and a new borate glass (CB) were developed and compared in terms of their in vitro behaviour both in acellular and cellular media. Acellular tests were carried out in SBF and the composites were characterized by SEM-EDS, XRD and ICP. Biocompatibility studies were investigated by in vitro cell culture with MG-63 osteoblast-like and human bone marrow cells. The growth of spherical calcium phosphate aggregates was observed in acellular medium on all composite surfaces indicating that these materials became potentially bioactive. The biological assessment resulted in a dissimilar behavior of the composites. The CSi demonstrated an inductive effect on the proliferation of cells. The cells showed a normal morphology and high growth rate when compared to standard culture plates. Contrarily, inhibition of cell proliferation occurred in the CB probably due to its high degradation rate, leading to high B and Mg ionic concentration in the cell culture medium.
authors.affiliation: CICECO and Department of Ceramics and Glass Engineering, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal.
authors.forename: P P
authors.initials: PP
authors.lastname: Lopes
authors.forename: B J M Leite
authors.initials: BJ
authors.lastname: Ferreira
authors.forename: P S
authors.initials: PS
authors.lastname: Gomes
authors.forename: R N
authors.initials: RN
authors.lastname: Correia
authors.forename: M H
authors.initials: MH
authors.lastname: Fernandes
authors.forename: M H V
authors.initials: MH
authors.lastname: Fernandes
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry / Q000494:pharmacology*; D001854:Bone Marrow Cells / Q000187:drug effects / Q000502:physiology; D001881:Borates / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003188:Composite Resins / Q000737:chemistry / Q000494:pharmacology*; D003793:Dental Restoration, Permanent; D005898:Glass / Q000737:chemistry; D006801:Humans; D008422:Materials Testing / Q000379:methods; D008768:Methylmethacrylates / Q000737:chemistry / Q000494:pharmacology; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000187:drug effects / Q000502:physiology; D011140:Polyurethanes / Q000737:chemistry / Q000494:pharmacology*; D017640:Silicates / Q000494:pharmacology*
chemical_list: D000180:Acrylic Resins; D001881:Borates; C029012:Composite Dental Resin; D003188:Composite Resins; D008768:Methylmethacrylates; D011140:Polyurethanes; D017640:Silicates; C539373:poly(methylmethacrylate)-co-(ethylhexylacrylate)