title: [Effect of S-allyl-L-cysteine on isolate heart subject to ischemia/reperfusion].
abstract: OBJECTIVE
  To investigate the effect of S-allyl-L-cysteine (SAC) on isolated rat heart subject to ischemia/reperfusion(I/R) injury and the mechanisms.
  
  
  METHODS
  The isolated perfused rat hearts on a Langendorff apparatus were subjected to global ischemia for 30 min and followed by 120 min of reperfusion. Hemodynamic index, the production of formazan and the level of lactate dehydrogenase (LDH) in the coronary effluent were determined. Superoxide dismutase (SOD) and reactive oxygen species (ROS) in myocardial homogenates were measured.
  
  
  RESULTS
  Compared with I/R group, the hemodynamics were greatly improved, the production of formazan was increased, and LDH level in effluent was reduced in SAC group. SAC improved the SOD activity and significantly decreased the level of ROS. In addition, threonine (Thr) attenuated the protective effect of SAC significantly.
  
  
  CONCLUSIONS
  SAC has protective effect against myocardial ischemia/reperfusion injury on rats. The possible mechanism is that SAC be transported into the cell through alanine-serine-cysteine-transporter 1 (ASCT-1) improves SOD activity and reduces the level of ROS.
authors.affiliation: Department of Physiology, Zhejiang University School of Medicine, Hangzhou 310058, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Xue
authors.forename: Jiea
authors.initials: J
authors.lastname: Cui
authors.forename: Wen
authors.initials: W
authors.lastname: Xia
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.forename: Ling-Bo
authors.initials: LB
authors.lastname: Qian
authors.forename: Zhi-Guo
authors.initials: ZG
authors.lastname: Ye
authors.forename: Hui-Ping
authors.initials: HP
authors.lastname: Wang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Xia
mesh_terms: D000818:Animals; D003545:Cysteine / Q000031:analogs & derivatives* / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D017202:Myocardial Ischemia / Q000503:physiopathology*; D015428:Myocardial Reperfusion Injury / Q000503:physiopathology / Q000517:prevention & control*; D020011:Protective Agents / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D020011:Protective Agents; D017382:Reactive Oxygen Species; C065299:S-allylcysteine; D013482:Superoxide Dismutase; D003545:Cysteine