title: Hepatocyte growth factor incorporated into herpes simplex virus vector accelerates facial nerve regeneration after crush injury.
abstract: Hepatocyte growth factor (HGF) promotes regeneration of the central nervous system, but its effects on the peripheral nervous system remain unclear. This study was conducted to elucidate the effect of HGF on regeneration of the murine facial nerve after crush injury. To do so, a replication-defective herpes simplex virus vector that incorporated HGF was prepared (HSV-HGF). The main trunk of the facial nerve was compressed by mosquito hemostats, and HSV-HGF, control vector or medium was then applied to the compressed nerve. We found that mice in the HGF group required significantly fewer days for complete recovery from nerve compression. Furthermore, the amplitude of the evoked buccinator muscle compound action potential increased following HSV-HGF application. HGF expression in and around the compressed nerve was demonstrated by enzyme-linked immunoassay and immunohistochemistry. In addition, HSV-HGF introduction around the damaged nerve significantly accelerated recovery of function of the facial nerve. These data suggest a possible role of HGF in promoting facial nerve regeneration after nerve damage. Furthermore, this viral delivery method may be applied clinically for many types of severe facial palsy during facial nerve decompression surgery.
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, Nagoya City University Graduate School of Medical Sciences and Medical School, Nagoya, Japan. sesaki@med.nagoya-cu.ac.jp
authors.forename: S
authors.initials: S
authors.lastname: Esaki
authors.forename: J
authors.initials: J
authors.lastname: Kitoh
authors.forename: S
authors.initials: S
authors.lastname: Katsumi
authors.forename: F
authors.initials: F
authors.lastname: Goshima
authors.forename: H
authors.initials: H
authors.lastname: Kimura
authors.forename: M
authors.initials: M
authors.lastname: Safwat
authors.forename: K
authors.initials: K
authors.lastname: Yamano
authors.forename: N
authors.initials: N
authors.lastname: Watanabe
authors.forename: N
authors.initials: N
authors.lastname: Nonoguchi
authors.forename: T
authors.initials: T
authors.lastname: Nakamura
authors.forename: R S
authors.initials: RS
authors.lastname: Coffin
authors.forename: S-I
authors.initials: SI
authors.lastname: Miyatake
authors.forename: Y
authors.initials: Y
authors.lastname: Nishiyama
authors.forename: S
authors.initials: S
authors.lastname: Murakami
mesh_terms: D000818:Animals; D005154:Facial Nerve / Q000502:physiology; D020220:Facial Nerve Injuries / Q000628:therapy*; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D017228:Hepatocyte Growth Factor / Q000235:genetics*; D051379:Mice; D009408:Nerve Compression Syndromes / Q000628:therapy; D009416:Nerve Regeneration / Q000187:drug effects* / Q000235:genetics; D018139:Simplexvirus / Q000235:genetics*
chemical_list: D017228:Hepatocyte Growth Factor