title: Biosynthesis of a novel recombinant peptide derived from hPTH(1-34).
abstract: A recombinant human parathyroid hormone fragment, Pro-Pro-[Arg(11)]hPTH(1-34)-Pro-Pro (MW=4550 Da), was developed by substituting Arg for Leu at position 11 and adding Pro-Pro at the carboxyl terminus. Following a single injection (0.5-13.5 μg/bird) of the rhPTH fragment, the serum calcium level in chickens increased 12-42% (P<0.05) after 1h as determined by the Parson's Chicken Assay. The functional activity of Pro-Pro-[Arg(11)]hPTH(1-34)-Pro-Pro may be due to removal of the N-terminus Pro-Pro- by X-prolyl dipeptidyl peptidase IV (DPPIV) in vivo, increasing its activity compared to Pro-Pro-hPTH(1-34). This artificial rhPTH fragment could be used to increase calcium mobilization and potentially improve bone health.
authors.affiliation: Laboratory of Minigene Pharmacy, School of Life Science and Technology, China Pharmaceutical University, Nanjing, China. sunflowerpetal@126.com
authors.forename: Wang
authors.initials: W
authors.lastname: Chunxiao
authors.forename: Liu
authors.initials: L
authors.lastname: Jingjing
authors.forename: Xiao
authors.initials: X
authors.lastname: Yire
authors.forename: Lu
authors.initials: L
authors.lastname: Jingning
authors.forename: Kang
authors.initials: K
authors.lastname: Kai
authors.forename: Shen
authors.initials: S
authors.lastname: Liang
authors.forename: Lu
authors.initials: L
authors.lastname: Yi
authors.forename: Barbara
authors.initials: B
authors.lastname: Rasco
mesh_terms: D000818:Animals; D002118:Calcium / Q000097:blood; D002645:Chickens; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D010281:Parathyroid Hormone / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000494:pharmacology*; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000494:pharmacology*; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D010281:Parathyroid Hormone; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; D002118:Calcium