title: Anti-CD3 and anti-cd16 monoclonal-antibody in-vitro enhancement of NK activity in pbl from gastrointestinal-tract cancer-patients.
abstract: Natural killer (NK) cell cytotoxicity was assessed using peripheral blood mononuclear cells from 103 healthy volunteers and 51 cancer patients. Peripheral blood cells were assessed by flow cytometry, and cytotoxicity in a standard 4-hour Cr-51-release assay using K562 cells as targets. Anti-CD3 and anti-CD16 mAb significantly enhanced cytoxicity in vitro. NK cell numbers correlated with levels of cytotoxicity. Patients with liver metastases had significantly more CD3+ lymphocytes coexpressing NK markers than patients without liver involvement. These CD3+ NK cells may also mediate cytotoxicity. Enhancement of cellular cytotoxicity by anti-CD3 and anti-CD16 may be of use in cancer immunotherapy protocols.
authors.affiliation: UNIV LEICESTER,LEICESTER ROYAL INFIRM,SCH MED,DEPT SURG,LEICESTER LE2 7LX,ENGLAND.
authors.forename: R
authors.initials: R
authors.lastname: Catterick
authors.forename: J
authors.initials: J
authors.lastname: Hollingworth
authors.forename: T
authors.initials: T
authors.lastname: Horsburgh
authors.forename: P
authors.initials: P
authors.lastname: Veitch
authors.forename: P
authors.initials: P
authors.lastname: Bell
authors.forename: R
authors.initials: R
authors.lastname: James