title: High performance thin layer chromatography determination of cellobiosan and levoglucosan in bio-oil obtained by fast pyrolysis of sawdust.
abstract: In this work, high performance thin layer liquid chromatography (HTPLC) is applied to the determination of sugars in fast pyrolysis liquids (bio-oil) and fractions thereof. The proposed procedure allows the separation of anhydrosugar levoglucosan and cellobiosan, as well as glucose, arabinose, xylose and cellobiose. Pre-treatment and derivatization of samples are not necessary and volatile compounds present in bio-oil do not interfere with sugar analysis. The detrimental effect of the complex bio-oil matrix on columns and detector lifetime is avoided by using disposable HTPLC plates. Prior screening of glucose, present especially in aged and aqueous bio-oil fractions, is required to quantify cellobiosan without interference. Concentrations of levoglucosan and cellobiosan in bio-oil samples obtained from Pinus radiata sawdust were ranged between 1.27-2.26% and 0.98-1.96% respectively, while a bio-oil sample obtained from native wood contained a higher levoglucosan concentration.
authors.affiliation: Departamento de Análisis Instrumental, Facultad de Farmacia, Universidad de Concepción, Casilla 237, Correo 3, Concepción, Chile.
authors.forename: Catherine
authors.initials: C
authors.lastname: Tessini
authors.forename: Mario
authors.initials: M
authors.lastname: Vega
authors.forename: Niels
authors.initials: N
authors.lastname: Müller
authors.forename: Luis
authors.initials: L
authors.lastname: Bustamante
authors.forename: Dietrich
authors.initials: D
authors.lastname: von Baer
authors.forename: Alex
authors.initials: A
authors.lastname: Berg
authors.forename: Claudia
authors.initials: C
authors.lastname: Mardones
mesh_terms: D056804:Biofuels / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002855:Chromatography, Thin Layer / Q000379:methods*; D004187:Disaccharides / Q000032:analysis* / Q000302:isolation & purification; D005947:Glucose / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification; D006358:Hot Temperature; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014934:Wood / Q000737:chemistry
chemical_list: D056804:Biofuels; D004187:Disaccharides; C524805:glucopyranosyl-(1-4)-1,6-anhydroglucopyranose; C014989:1,6-anhydro-beta-glucopyranose; D005947:Glucose