title: Characterization of 15 microsatellite loci in the pulmonate snail Pseudosuccinea columella (Mollusca, Gastropoda).
abstract: We characterized 15 new variable microsatellites in the freshwater snail Pseudosuccinea (Lymnaea) columella, as well as conditions for multiplexing and simultaneously genotyping sets of loci. Two to six alleles were detected per locus over the six populations studied. Gene diversity ranged from 0.000 to 0.498, but essentially no heterozygous individuals were observed. This resulted in extremely high F(IS) estimates, and therefore high selfing rates. The F(ST) estimates ranged from 0.18 to 1 among populations, but was generally high. These markers will constitute efficient tools for investigating the population structure of this invasive species. Cross-species amplification was on the whole unsuccessful.
authors.affiliation: CEFE, UMR 5175 CNRS, 1919 route de Mende, 34293 Montpellier cedex 5, France.
authors.forename: Antoine
authors.initials: A
authors.lastname: Nicot
authors.forename: Marie-Pierre
authors.initials: MP
authors.lastname: Dubois
authors.forename: Chantal
authors.initials: C
authors.lastname: Debain
authors.forename: Patrice
authors.initials: P
authors.lastname: David
authors.forename: Philippe
authors.initials: P
authors.lastname: Jarne