title: Cyanines as efficient photosensitizers in photodynamic reaction: photophysical properties and in vitro photodynamic activity.
abstract: The purpose of the present study was to explore the potential application of cyanines in photodynamic treatment. The photophysical features of four cyanines (KF570, HM118, FBF-749, and ER-139) were investigated by elemental and spectral analyses. Two malignant cell lines (MCF-7/WT and MCF-7/DOX) were used to test the potential for use in the photodynamic therapy. The cytotoxic effects of these dyes were determined by the MTT assay after 4 and 24 h of incubation with the cyanine. KF570 and HM118 were irradiated with red light (630-nm filter) and FBF-749 and ER-139 with green light (435-nm filter). The results showed that the cyanine HM118 demonstrated a major phototoxic effect. It was also noted that the efficiency of photodynamic therapy was higher in the doxorubicin-resistant cell line (MCF-7/DOX).
authors.affiliation: Medical University, Department of Medical Biochemistry, Wroclaw, Poland. jkulbacka@gmail.com
authors.forename: J
authors.initials: J
authors.lastname: Kulbacka
authors.forename: A
authors.initials: A
authors.lastname: Pola
authors.forename: D
authors.initials: D
authors.lastname: Mosiadz
authors.forename: A
authors.initials: A
authors.lastname: Choromanska
authors.forename: P
authors.initials: P
authors.lastname: Nowak
authors.forename: M
authors.initials: M
authors.lastname: Kotulska
authors.forename: M
authors.initials: M
authors.lastname: Majkowski
authors.forename: A
authors.initials: A
authors.lastname: Hryniewicz-Jankowska
authors.forename: L
authors.initials: L
authors.lastname: Purzyc
authors.forename: J
authors.initials: J
authors.lastname: Saczko
mesh_terms: D001578:Benzopyrans / Q000737:chemistry / Q000494:pharmacology*; D002232:Carbocyanines / Q000737:chemistry / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D005453:Fluorescence; D006801:Humans; D007211:Indoles / Q000737:chemistry / Q000494:pharmacology*; D008081:Liposomes; D010713:Phosphatidylcholines; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000737:chemistry / Q000494:pharmacology*; D053778:Thiazolidines / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D001578:Benzopyrans; D002232:Carbocyanines; D007211:Indoles; D008081:Liposomes; D010713:Phosphatidylcholines; D017319:Photosensitizing Agents; D053778:Thiazolidines; C548873:merocyanine