title: Tumor vascular shutdown following photodynamic therapy based on polyhematoporphyrin or 5-aminolevulinic Acid.
abstract: The effects of photodynamic therapy (PDT) based on polyhaematoporphyrin (PHP) or 5-aminolaevulinic acid (ALA) on the tumour vascular perfusion (TVP) of a rat fibrosarcoma were examined using an (RbCl)-Rb-86 extraction technique. Both forms of therapy induced large and highly significant reductions in TVP, countering the previous suggestion that ALA-PDT has no vascular effects. Ultrasensitive digital imaging fluorescence microscopy indicated that ALA-induced PpIX was distributed relatively evenly throughout the tumour. Use of Hoechst 33342 as a vascular marker revealed that PpIX was also present in cells lining the tumour microvascular spaces, contrary to previous findings.
authors.affiliation: COOKRIDGE HOSP,DEPT CLIN ONCOL,LEEDS LS16 6QB,W YORKSHIRE,ENGLAND. COOKRIDGE HOSP,DEPT MED PHYS,LEEDS LS16 6QB,W YORKSHIRE,ENGLAND. UNIV LEEDS,DEPT BIOCHEM & MOLEC BIOL,LEEDS LS2 9JT,W YORKSHIRE,ENGLAND.
authors.forename: D
authors.initials: D
authors.lastname: Roberts
authors.forename: F
authors.initials: F
authors.lastname: Cairnduff
authors.forename: I
authors.initials: I
authors.lastname: Driver
authors.forename: B
authors.initials: B
authors.lastname: Dixon
authors.forename: S
authors.initials: S
authors.lastname: Brown