title: [Construction and expression of the eukaryotic expression vector carrying HSV-1 gC glycoprotein gene].
abstract: OBJECTIVE
  To stably express herpes simplex virus type 1 (HSV-1) glycoprotein C (gC) in Chinese hamster ovary cells (CHO-K1).
  
  
  METHODS
  The eukaryotic expression vector pCI-mCMV-gC-1-IRES-DHFR-L22R was constructed and transfected into CHO-K1 cells by Lipofectamine 2000. The transfected cells were selected by G418 and methotrexate (MTX). The expression of HSV-1 gC was analyzed by Slot blot. HSV-1 gC proteins were purified with His-Ni Sepharose and then detected by Western blot.
  
  
  RESULTS
  The eukaryotic expression vector pCI-mCMV-gC-1-IRES-DHFR-L22R was constructed successfully. CHO-K1 cells stably expressing HSV-1 gC proteins were established and confirmed by Western blot.
  
  
  CONCLUSIONS
  The HSV-1 gC proteins have been expressed successfully and have good bioactivity. The results make it possible for further study and clinical use of HSV-1 gC.
authors.affiliation: Department of Microbiology, Fourth Military Medical University, Xi'an 710032, China. dangyingli2005@163.com
authors.forename: Yin-li
authors.initials: YL
authors.lastname: Dang
authors.forename: Yan
authors.initials: Y
authors.lastname: Yan
authors.forename: Xiao-xiao
authors.initials: XX
authors.lastname: Zhang
authors.forename: Pu-yuan
authors.initials: PY
authors.lastname: Li
authors.forename: Lan
authors.initials: L
authors.lastname: Yu
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Fang-lin
authors.initials: FL
authors.lastname: Zhang
authors.forename: Zhi-kai
authors.initials: ZK
authors.lastname: Xu
authors.forename: Xing-an
authors.initials: XA
authors.lastname: Wu
mesh_terms: D000818:Animals; D016466:CHO Cells; D003001:Cloning, Molecular; D006224:Cricetinae; D003412:Cricetulus; D005822:Genetic Vectors / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D014759:Viral Envelope Proteins / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D014759:Viral Envelope Proteins; C038535:glycoprotein gC, herpes simplex virus type 1