title: Isolation of a polysaccharide with antiproliferative, hypoglycemic, antioxidant and HIV-1 reverse transcriptase inhibitory activities from the fruiting bodies of the abalone mushroom Pleurotus abalonus.
abstract: OBJECTIVE
  The intent of this study was to purify and characterize a polysaccharide named LA from the fruiting bodies of the edible mushroom Pleurotus abalones.
  
  
  METHODS
  The 120-kDa polysaccharide was obtained by extraction with boiling water, ethanol precipitation, ion exchange chromatography on Diethylaminoethyl-cellulose and gel filtration on Sephadex G-200.
  
  
  RESULTS
  The LA was composed of glucose, rhamnose, glucuronic acid, xylose, galactose and arabinose in the molar ratio of 26.3:2.7:1:1.4:1.8:1.2. The FITR and 1H-NMR spectrum of LA disclosed that it was a saccharide with an α-configuration. Its 13C-NMR spectrum revealed that its main chain was [→6)-α-D-Gly(1→]n. The LA exhibited antioxidant activities, especially in scavenging 1,1- diphenyl-2-picryl-hydrazyl radicals and hydroxyl radicals. It manifested antiproliferative activity towards breast cancer MCF7 cells with an IC50 of 3.7 µm, and also exerted some antiproliferative activity against HepG2 cells. It manifested a hypoglycemic action on diabetic mice. It inhibited HIV-1 reverse transcriptase with an IC50 of 8.7 × 10(-2) µm.
  
  
  CONCLUSIONS
  The polysaccharide from the abalone mushroom.
authors.affiliation: Center for AIDS Research, Department of Microbiology, Nankai University, Tianjin, China.
authors.forename: Chang Rong
authors.initials: CR
authors.lastname: Wang
authors.forename: Tzi Bun
authors.initials: TB
authors.lastname: Ng
authors.forename: Le
authors.initials: L
authors.lastname: Li
authors.forename: Jin Cen
authors.initials: JC
authors.lastname: Fang
authors.forename: Yun
authors.initials: Y
authors.lastname: Jiang
authors.forename: Ting Yi
authors.initials: TY
authors.lastname: Wen
authors.forename: Wen Tao
authors.initials: WT
authors.lastname: Qiao
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
mesh_terms: D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D001688:Biological Products / Q000494:pharmacology* / Q000627:therapeutic use; D001713:Biphenyl Compounds / Q000378:metabolism; D001943:Breast Neoplasms / Q000188:drug therapy; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003920:Diabetes Mellitus / Q000139:chemically induced / Q000188:drug therapy; D005260:Female; D048690:Fruiting Bodies, Fungal; D054303:HIV Reverse Transcriptase / Q000187:drug effects; D056945:Hep G2 Cells; D006801:Humans; D017665:Hydroxyl Radical / Q000378:metabolism; D006943:Hyperglycemia / Q000139:chemically induced / Q000188:drug therapy; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D020128:Inhibitory Concentration 50; D008113:Liver Neoplasms / Q000188:drug therapy; D008517:Phytotherapy; D010851:Picrates / Q000378:metabolism; D020076:Pleurotus / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018894:Reverse Transcriptase Inhibitors / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D001688:Biological Products; D001713:Biphenyl Compounds; D007004:Hypoglycemic Agents; D010851:Picrates; D011134:Polysaccharides; D018894:Reverse Transcriptase Inhibitors; D017665:Hydroxyl Radical; C004931:1,1-diphenyl-2-picrylhydrazyl; C514824:reverse transcriptase, Human immunodeficiency virus 1; D054303:HIV Reverse Transcriptase