title: Enzymatic glutathione production using metabolically engineered Saccharomyces cerevisiae as a whole-cell biocatalyst.
abstract: We developed a novel enzymatic glutathione (GSH) production system using Saccharomyces cerevisiae as a whole-cell biocatalyst, and improved its GSH productivity by metabolic engineering. We demonstrated that the metabolic engineering of GSH pathway and ATP regeneration can significantly improve GSH productivity by up to 1.7-fold higher compared with the parental strain, respectively. Furthermore, the combination of both improvements in GSH pathway and ATP regeneration is more effective (2.6-fold) than either improvement individually for GSH enzymatic production using yeast. The improved whole-cell biocatalyst indicates its great potential for applications to other kinds of ATP-dependent bioproduction.
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan.
authors.forename: Hideyo
authors.initials: H
authors.lastname: Yoshida
authors.forename: Kiyotaka Y
authors.initials: KY
authors.lastname: Hara
authors.forename: Kentaro
authors.initials: K
authors.lastname: Kiriyama
authors.forename: Hideki
authors.initials: H
authors.lastname: Nakayama
authors.forename: Fumiyoshi
authors.initials: F
authors.lastname: Okazaki
authors.forename: Fumio
authors.initials: F
authors.lastname: Matsuda
authors.forename: Chiaki
authors.initials: C
authors.lastname: Ogino
authors.forename: Hideki
authors.initials: H
authors.lastname: Fukuda
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kondo
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D004798:Enzymes / Q000378:metabolism*; D005818:Genetic Engineering; D005978:Glutathione / Q000378:metabolism*; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*
chemical_list: D004798:Enzymes; D000255:Adenosine Triphosphate; D005978:Glutathione