title: Coherent anti-Stokes Raman scattering hyperspectral tissue imaging with a wavelength-swept system.
abstract: We present a wavelength-swept coherent anti-Stokes Raman scattering (WS-CARS) spectroscopy system for hyperspectral imaging in thick tissue. We use a strategy where the Raman lines are excited sequentially, circumventing the need for a spectrometer. This fibre laser system, consisting of a pump laser synchronized with a rapidly tunable programmable laser (PL), can access Raman lines over a significant fraction of the high wavenumber region (2700-2950 cm(-1)) at rates of up to 10,000 spectral points per second. To demonstrate its capabilities, we have acquired WS-CARS spectra of several samples as well as images and hyperspectral images (HSI) of thick tissue both in forward and epi-detection. This instrument should be especially useful in providing local biochemical information with surrounding context supplied by imaging.
authors.forename: Steve
authors.initials: S
authors.lastname: Bégin
authors.forename: Bryan
authors.initials: B
authors.lastname: Burgoyne
authors.forename: Vincent
authors.initials: V
authors.lastname: Mercier
authors.forename: Alain
authors.initials: A
authors.lastname: Villeneuve
authors.forename: Réal
authors.initials: R
authors.lastname: Vallée
authors.forename: Daniel
authors.initials: D
authors.lastname: Côté