title: [Expression of TGF-β1 and PCNA in cyclosporin A-induced gingival overgrowth].
abstract: OBJECTIVE
  To investigate the expression of transforming growth factor-β1(TGF-β1) and proliferating cell nuclear of antigen (PCNA) in cyclosporin A(CsA)-induced gingival overgrowth (GO) tissues.
  
  
  METHODS
  After extraction of half of the maxillary molars, 20 male Sprague-Dawley rats were randomized into a CsA-injection group (10mg·kg⁻·d⁻¹) or a normal saline control group. Ten rats per group were sacrificed at the fourth week after injection. The gingival width was measured under a stereo-microscope. After full demineralization of the mandible, gingival specimens were taken. The linear measurements and the immunohistochemistry analysis of TGF-β1 and PCNA were made using the software of Image Pro-plus 6.0. The data was analyzed with SAS6.12 software package.
  
  
  RESULTS
  The percentage of cells positive staining for TGF-β1 and PCNA was significantly higher in the CsA rats than in the control rats (P<0.05), but there was no significant difference between the edentulous and denti-gingiva in the same group(P>0.05).
  
  
  CONCLUSIONS
  The upregulation of TGF-β1 may have no direct relations with gingival inflammation in CsA-induced gingival overgrowth.In addition, TGF-β1 may be one of the key cytokines for CsA-induced gingival overgrowth. Increased epithelial proliferative activity may be the main cause of epithelial hyperplasia.
authors.affiliation: Department of Periodontics, Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine,Shanghai Key Laboratory of Stomatology, Shanghai 200011, China. trili2006@163.com
authors.forename: Li-li
authors.initials: LL
authors.lastname: Li
authors.forename: Yuan-zheng
authors.initials: YZ
authors.lastname: Yin
authors.forename: Zhong-kai
authors.initials: ZK
authors.lastname: Wu
authors.forename: Yuan-jie
authors.initials: YJ
authors.lastname: Shi
mesh_terms: D000818:Animals; D016572:Cyclosporine; D005881:Gingiva; D019214:Gingival Overgrowth; D005891:Gingivitis; D007166:Immunosuppressive Agents; D008297:Male; D018809:Proliferating Cell Nuclear Antigen; D051381:Rats; D017207:Rats, Sprague-Dawley; D053773:Transforming Growth Factor beta1
chemical_list: D007166:Immunosuppressive Agents; D018809:Proliferating Cell Nuclear Antigen; D053773:Transforming Growth Factor beta1; D016572:Cyclosporine