title: New polymorphic microsatellite loci for the Macedonian crested newt, Triturus macedonicus, and cross-priming testing in four other crested newt species.
abstract: Eleven microsatellites have been characterized for Triturus macedonicus. Nine loci showed different variation patterns in a sample of 40 individuals from a single breeding pond in Zagori province (Greece), with an average number of 4.2 alleles per locus and an expected heterozygosity ranging from 0.1199 to 0.8079. Distinct cross-priming amplification rates were recovered on four additional crested newt species. Two monomorphic T. macedonicus loci were polymorphic in other Triturus species. The microsatellites developed herein could be a useful intraspecific genetic tool to undertake fine-scale population genetic analyses as well as in the study of contact zones between crested newt species.
authors.affiliation: Section of Zoology-Marine Biology, Department of Biology, University of Athens, Panepistimioupolis, 157 84 Athens, Greece, Institute of Marine Biology and Genetics, Hellenic Centre for Marine Research, PO Box 2214, 71003 Heraklion, Greece, Zoological Museum, Department of Biology, University of Athens, Panepistimioupolis, 157 84 Athens, Greece.
authors.forename: K
authors.initials: K
authors.lastname: Sotiropoulos
authors.forename: D
authors.initials: D
authors.lastname: Tsaparis
authors.forename: K
authors.initials: K
authors.lastname: Eleftherakos
authors.forename: G
authors.initials: G
authors.lastname: Kotoulas
authors.forename: A
authors.initials: A
authors.lastname: Legakis
authors.forename: P
authors.initials: P
authors.lastname: Kasapidis