title: Biofunctionalization of polyelectrolyte microcapsules with biotinylated polyethylene glycol-grafted liposomes.
abstract: Hollow polyelectrolyte microcapsules (PEMC) are prepared using layer-by-layer self-assembly of polyelectrolytes on melamine formaldehyde templates, followed by template dissolution, and subsequent coating with biotinylated polyethylene glycol-grafted liposomes. These potential site-specific carrier systems show a high specificity for NeutrAvidin binding and a strong resistance against unspecific protein binding. It is concluded that this design with NeutrAvidin as the outermost layer of such capsules provides an ideal platform for the biofunctionalization of PEMC as drug delivery systems or as artificial cell-like structures for biomimetic studies.
authors.affiliation: School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore.
authors.forename: Jie
authors.initials: J
authors.lastname: Gao
authors.forename: Uta
authors.initials: U
authors.lastname: Reibetanz
authors.forename: Subbu
authors.initials: S
authors.lastname: Venkatraman
authors.forename: Björn
authors.initials: B
authors.lastname: Neu
mesh_terms: D058045:Artificial Cells / Q000737:chemistry*; D001360:Avidin / Q000737:chemistry / Q000378:metabolism; D001672:Biocompatible Materials / Q000032:analysis / Q000737:chemistry*; D032701:Biomimetics / Q000379:methods*; D001710:Biotin / Q000737:chemistry / Q000378:metabolism*; D019921:Biotinylation; D002214:Capsules / Q000032:analysis / Q000737:chemistry*; D004337:Drug Carriers / Q000032:analysis / Q000737:chemistry*; D004573:Electrolytes / Q000737:chemistry; D005434:Flow Cytometry; D006801:Humans; D008081:Liposomes / Q000032:analysis / Q000737:chemistry; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D011092:Polyethylene Glycols / Q000737:chemistry; D014227:Triazines / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D002214:Capsules; D004337:Drug Carriers; D004573:Electrolytes; D008081:Liposomes; D014227:Triazines; C504353:melamine formaldehyde; D001360:Avidin; D011092:Polyethylene Glycols; D001710:Biotin