title: HIV-1 remodels the nuclear pore complex.
abstract: Human immunodeficiency virus type 1 (HIV-1) commandeers host cell proteins and machineries for its replication. Our earlier work showed that HIV-1 induced the cytoplasmic retention of nucleocytoplasmic shuttling and ribonucleic acid (RNA)-binding proteins. This retention is dependent on nuclear export of the viral genomic RNA and on changes in the localization and expression level of the nucleoporin (Nup) p62 (Nup62). To further characterize the extent of perturbation induced by HIV-1, we performed proteomics analyses of nuclear envelopes (NEs) isolated from infected T cells. Infection induced extensive changes in the composition of the NE and its associated proteins, including a remarkable decrease in the abundance of Nups. Immunogold electron microscopy revealed the translocation of Nups into the cytoplasm. Nup62 was identified as a component of purified virus, and small interfering RNA depletion studies revealed an important role for this Nup in virus gene expression and infectivity. This detailed analysis highlights the profound effects on NE composition induced by HIV-1 infection, providing further evidence of the magnitude of viral control over the cell biology of its host.
authors.affiliation: HIV-1 RNA Trafficking Laboratory, Lady Davis Institute for Medical Research, Sir Mortimer B. Davis Jewish General Hospital, Montréal, Québec H3T 1E2, Canada.
authors.forename: Anne
authors.initials: A
authors.lastname: Monette
authors.forename: Nelly
authors.initials: N
authors.lastname: Panté
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Mouland
mesh_terms: D021581:Active Transport, Cell Nucleus; D053263:Gene Regulatory Networks; D015497:HIV-1 / Q000235:genetics / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D019169:Jurkat Cells; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D022022:Nuclear Pore / Q000378:metabolism / Q000821:virology*; D028861:Nuclear Pore Complex Proteins / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D034622:RNA Interference; D012367:RNA, Viral / Q000378:metabolism; D012261:Ribonucleoproteins / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism / Q000821:virology*; D014162:Transfection; D014779:Virus Replication
chemical_list: D008562:Membrane Glycoproteins; D028861:Nuclear Pore Complex Proteins; D012367:RNA, Viral; D012261:Ribonucleoproteins; C049312:nuclear pore protein p62