title: Laboratory and field evaluation of the transgenic Populus alba × Populus glandulosa expressing double coleopteran-resistance genes.
abstract: Expression of the two coleopteran-resistant proteins (Bt-Cry3A and oryzacystatin I) was detected in the leaves of field-grown transgenic poplar (BOGA-5) in two or three subsequent years. The BOGA-5 contained ∼10 μg g(-1) of Cry3A over the individual years with no detection in the control, and protein extracts from BOGA-5 displayed a higher reduction in papain activity (∼42%) compared with ∼21% in the control. Laboratory feeding experiments showed that the total mortality of the target pest Plagiodera versicolora (Coleoptera, Chrysomelida) larvae fed with BOGA-5 leaves was 76.7%, significantly higher than that of the control (P< .05). However, no significant differences were detected in the mortality, exuviation index, pupation rate or adult eclosion rate of the non-target Clostera anachoreta (Lepidoptera, Notodontidae) fed with leaves from transgenic and non-transgenic poplars. Field investigation indicated that the transgenic poplar retained coleopteran insect resistance in the field, suggesting the potential use of the double gene transgenic poplar for pest management in commercial poplar plantations.
authors.affiliation: Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, PR China.
authors.forename: Bingyu
authors.initials: B
authors.lastname: Zhang
authors.forename: Min
authors.initials: M
authors.lastname: Chen
authors.forename: Xiaofen
authors.initials: X
authors.lastname: Zhang
authors.forename: Hehui
authors.initials: H
authors.lastname: Luan
authors.forename: Shu
authors.initials: S
authors.lastname: Diao
authors.forename: Yingchuan
authors.initials: Y
authors.lastname: Tian
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Su
mesh_terms: D000818:Animals; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D001517:Coleoptera; D015891:Cystatins / Q000096:biosynthesis / Q000235:genetics*; D004731:Endotoxins / Q000096:biosynthesis / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D006460:Hemolysin Proteins / Q000096:biosynthesis / Q000235:genetics*; D007300:Insect Control / Q000379:methods*; D007814:Larva; D007915:Lepidoptera; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000378:metabolism; D032107:Populus / Q000235:genetics* / Q000378:metabolism
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D015891:Cystatins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis