title: Isolation and characterization of polymorphic microsatellite loci in the freshwater fishes Telestes souffia and Telestes muticellus (Teleostei: Cyprinidae).
abstract: Ten novel polymorphic microsatellites (seven with perfect motifs) were isolated from vairone species (Telestes souffia and Telestes muticellus), which are endangered European cyprinid species. Together with 11 previously published cyprinid-specific loci, five multiplex sets were optimized, allowing the genotyping of 21 polymorphic loci. The level of genetic diversity was assessed in 97 individuals from the two species T. souffia and T. muticellus. We also successfully applied the 21 microsatellites to nine related species. These primers will thus be useful in assessing population structure of the vairone and other cyprinid species, with application for conservation issues and phylogeographical approaches.
authors.affiliation: Aix-Marseille Université, CNRS, IRD, UMR 6116 - IMEP, Equipe Persistance et Evolution de la Biodiversité, Centre Saint-Charles, Case 36, 3 Place Victor Hugo, 13331 Marseille cedex 3, France Montpellier SupAgro, Centre de Biologie et de Gestion des Populations, Campus International de Baillarguet, CS30016, 34988 Montferrier sur Lez, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Dubut
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Martin
authors.forename: Caroline
authors.initials: C
authors.lastname: Costedoat
authors.forename: Rémi
authors.initials: R
authors.lastname: Chappaz
authors.forename: André
authors.initials: A
authors.lastname: Gilles