title: Foot and mouth disease virus polyepitope protein produced in bacteria and plants induces protective immunity in guinea pigs.
abstract: The goal of this project was to develop an alternative foot and mouth disease (FMD) vaccine candidate based on a recombinant protein consisting of efficient viral epitopes. A recombinant gene was designed that encodes B-cell epitopes of proteins VP1 and VP4 and T-cell epitopes of proteins 2C and 3D. The polyepitope protein (H-PE) was produced in E. coli bacteria or in N. benthamiana plants using a phytovirus expression system. The methods of extraction and purification of H-PE proteins from bacteria and plants were developed. Immunization of guinea pigs with the purified H-PE proteins induced an efficient immune response against foot and mouth disease virus (FMDV) serotype O/Taiwan/99 and protection against the disease. The polyepitope protein H-PE can be used as a basis for developing a new recombinant vaccine against FMD.
authors.affiliation: Bioengineering Center, Russian Academy of Sciences, Moscow, Russia.
authors.forename: E P
authors.initials: EP
authors.lastname: Andrianova
authors.forename: S R
authors.initials: SR
authors.lastname: Krementsugskaia
authors.forename: N N
authors.initials: NN
authors.lastname: Lugovskaia
authors.forename: T K
authors.initials: TK
authors.lastname: Mayorova
authors.forename: V V
authors.initials: VV
authors.lastname: Borisov
authors.forename: M A
authors.initials: MA
authors.lastname: Eldarov
authors.forename: N V
authors.initials: NV
authors.lastname: Ravin
authors.forename: A S
authors.initials: AS
authors.lastname: Folimonov
authors.forename: K G
authors.initials: KG
authors.lastname: Skryabin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D000939:Epitopes / Q000276:immunology; D004926:Escherichia coli / Q000166:cytology / Q000235:genetics*; D029544:Foot-and-Mouth Disease Virus / Q000276:immunology*; D006168:Guinea Pigs; D008969:Molecular Sequence Data; D014026:Nicotiana / Q000166:cytology / Q000235:genetics*; D014614:Vaccines, Synthetic / Q000096:biosynthesis / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification; D014764:Viral Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D014765:Viral Vaccines / Q000096:biosynthesis* / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification
chemical_list: D000939:Epitopes; D014614:Vaccines, Synthetic; D014764:Viral Proteins; D014765:Viral Vaccines