title: The effect of fgf-3 int-2 on growth and transformation of mcf-10a normal human mammary epithelial-cells is distinct from fgf-1 and fgf-2.
abstract: Fibroblast growth factors (FGFs) are related polypeptides with mitogenic activity on cells of mesodermal and neuroectodermal origin. The Fgf-3 gene shares high homology with FGF-2 and its protein product can substitute FGF-2 as a growth factor. Other observations, however, indicate that Fgf-3 has specialized functions. We have investigated the effect of the expression and secretion of Fgf-3 on the growth and transformation of the human breast epithelial cell line MCF-10A. Overexpression of Fgf-3 stimulates proliferation of these cells in serum-free medium and induces anchorage-independent colony formation in soft agar. In contrast, these effects were not observed with purified FGF-1 and FGF-2 on either the parental or the Fgf-3-MCF-10A cells. Thus, Fgf-3 is distinct from FGF-1 and FGF-2 for its ability to induce cell proliferation and transformation of MCF-10A cells. This difference could be due, at least in part, to the expression of a specific set of FGF receptors with higher affinity for FGF-3 than FGF-1 or FGF-2.
authors.affiliation: S GIOVANNI HOSP, ANAT PATHOL SECT, I-10123 TURIN, ITALY. UNIV NAPOLI FEDERICO II, CATTEDRA ONCOL MED, I-80131 NAPLES, ITALY. UNIV PAVIA, SCH MED, INST MED & PUBL HLTH, I-21100 VARESE, ITALY. FRIEDRICH MIESCHER INST, CH-4002 BASEL, SWITZERLAND.
authors.forename: F
authors.initials: F
authors.lastname: Basolo
authors.forename: T
authors.initials: T
authors.lastname: Venesio
authors.forename: S
authors.initials: S
authors.lastname: Calvo
authors.forename: L
authors.initials: L
authors.lastname: Fiore
authors.forename: G
authors.initials: G
authors.lastname: Fontanini
authors.forename: F
authors.initials: F
authors.lastname: Ciardiello
authors.forename: A
authors.initials: A
authors.lastname: Toniolo
authors.forename: D
authors.initials: D
authors.lastname: Liscia
authors.forename: G
authors.initials: G
authors.lastname: Merlo