title: Expression of superoxide dismutase (SOD) genes from the copper-exposed polychaete, Neanthes succinea.
abstract: To evaluate the superoxide dismutase (SOD) genes as a potential indicator of environmental stress, we cloned and sequenced the full-length cDNA of a Cu/Zn-SOD and Mn-SOD genes from the marine polychaete, Neanthes succinea. Ns-Cu/Zn- and Mn-SOD expression was measured using quantitative real-time RT-PCR in response to the oxidative stress induced by copper in the form of CuCl2. Ns-Cu/Zn-SOD expression increased in a dose dependent manner but the increase of Mn-SOD expression was evident only at a high concentration of copper. Particularly, Ns-Mn-SOD dramatically increased in the 72 μg/L CuCl2-exposed groups, indicating that both Ns-SOD genes were induced by copper exposure. These results suggest that Ns-Cu/Zn- and Ns-Mn-SOD would play important roles in the detoxification of cellular oxidative stresses in the polychaete, N. succinea. Furthermore, as part of field work, the marine polychaete, N. succineaCu/Zn-SOD mRNA collected from the polluted area was accordingly upregulated in expression but not for NS-Mn-SOD.
authors.affiliation: Department of Marine Molecular and Environmental Bioscience, Graduate School, Hanyang University, Seoul 133-791, South Korea.
authors.forename: Jae-Sung
authors.initials: JS
authors.lastname: Rhee
authors.forename: Eun-Ji
authors.initials: EJ
authors.lastname: Won
authors.forename: Ryeo-Ok
authors.initials: RO
authors.lastname: Kim
authors.forename: Jehee
authors.initials: J
authors.lastname: Lee
authors.forename: Kyung-Hoon
authors.initials: KH
authors.lastname: Shin
authors.forename: Jae-Seong
authors.initials: JS
authors.lastname: Lee
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003300:Copper / Q000633:toxicity*; D015870:Gene Expression / Q000187:drug effects; D008969:Molecular Sequence Data; D018384:Oxidative Stress; D011077:Polychaeta / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D013482:Superoxide Dismutase / Q000235:genetics* / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D012333:RNA, Messenger; D014874:Water Pollutants, Chemical; D003300:Copper; D013482:Superoxide Dismutase