title: Antibodies reactive to non-HLA antigens in transplant glomerulopathy.
abstract: Although T and B cell alloimmunity contribute to transplant injury, autoimmunity directed at kidney-expressed, non-HLA antigens may also participate. Because the specificity, prevalence, and importance of antibodies to non-HLA antigens in late allograft injury are poorly characterized, we used a protein microarray to compare antibody repertoires in pre- and post-transplant sera from several cohorts of patients with and without transplant glomerulopathy. Transplantation routinely induced changes in antibody repertoires, but we did not identify any de novo non-HLA antibodies common to patients with transplant glomerulopathy. The screening studies identified three reactivities present before transplantation that persisted after transplant and strongly associated with transplant glomerulopathy. ELISA confirmed that reactivity against peroxisomal-trans-2-enoyl-coA-reductase strongly associated with the development of transplant glomerulopathy in independent validation sets. In addition to providing insight into effects of transplantation on non-HLA antibody repertoires, these results suggest that pretransplant serum antibodies to peroxisomal-trans-2-enoyl-coA-reductase may predict prognosis in kidney transplantation.
authors.affiliation: Department of Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA. rajani.dinavahi@mssm.edu
authors.forename: Rajani
authors.initials: R
authors.lastname: Dinavahi
authors.forename: Ajish
authors.initials: A
authors.lastname: George
authors.forename: Anne
authors.initials: A
authors.lastname: Tretin
authors.forename: Enver
authors.initials: E
authors.lastname: Akalin
authors.forename: Scott
authors.initials: S
authors.lastname: Ames
authors.forename: Jonathan S
authors.initials: JS
authors.lastname: Bromberg
authors.forename: Graciela
authors.initials: G
authors.lastname: Deboccardo
authors.forename: Nicholas
authors.initials: N
authors.lastname: Dipaola
authors.forename: Susan M
authors.initials: SM
authors.lastname: Lerner
authors.forename: Anita
authors.initials: A
authors.lastname: Mehrotra
authors.forename: Barbara T
authors.initials: BT
authors.lastname: Murphy
authors.forename: Tibor
authors.initials: T
authors.lastname: Nadasdy
authors.forename: Estela
authors.initials: E
authors.lastname: Paz-Artal
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Salomon
authors.forename: Bernd
authors.initials: B
authors.lastname: Schröppel
authors.forename: Vinita
authors.initials: V
authors.lastname: Sehgal
authors.forename: Ravi
authors.initials: R
authors.lastname: Sachidanandam
authors.forename: Peter S
authors.initials: PS
authors.lastname: Heeger
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000906:Antibodies / Q000097:blood* / Q000276:immunology; D016022:Case-Control Studies; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007678:Kidney Glomerulus / Q000276:immunology / Q000473:pathology*; D016030:Kidney Transplantation / Q000276:immunology / Q000473:pathology*; D008875:Middle Aged; D044925:Oxidoreductases Acting on CH-CH Group Donors / Q000276:immunology*; D011237:Predictive Value of Tests; D011379:Prognosis; D040081:Protein Array Analysis; D055815:Young Adult
chemical_list: D000906:Antibodies; D044925:Oxidoreductases Acting on CH-CH Group Donors; C434934:trans-2-enoyl-CoA reductase (NADPH)