title: Electrophoretic fingerprint metallothionein analysis as a potential prostate cancer biomarker.
abstract: Prostate-specific antigen (PSA) is a routinely used marker of prostate cancer; however, the cut-off values for unambiguous positive/negative prostate cancer diagnoses are not defined. Therefore, despite the best effort, certain percentage of misdiagnosed cases is being recorded every year. For this reason, search for more specific diagnostic markers is of great interest. In this study, systematic comparison of PSA and metallothionein (MT) levels in blood serum of 46 prostate cancer-diagnosed patients is presented. It is clearly demonstrated that PSA levels vary significantly and despite normal total PSA values in the range of 0 - 4 ng/mL were obtained in over 36.9% of cases, positive prostate cancer was diagnosed by biopsy. In contrary, MT levels were considerably elevated in all tested samples and no significant variations were observed. These results are indicating the potential of MT as an additional prostate cancer marker reducing, in combination with PSA, the probability of false positive/negative diagnosis. To increase the throughput of the screening, chip-based capillary electrophoresis was suggested as a rapid and effective method for the fingerprinting analysis of prostate cancer from diseased blood sera.
authors.affiliation: Department of Chemistry and Biochemistry, Mendel University in Brno, Brno, Czech Republic.
authors.forename: Sona
authors.initials: S
authors.lastname: Krizkova
authors.forename: Marketa
authors.initials: M
authors.lastname: Ryvolova
authors.forename: Jaromir
authors.initials: J
authors.lastname: Gumulec
authors.forename: Michal
authors.initials: M
authors.lastname: Masarik
authors.forename: Vojtech
authors.initials: V
authors.lastname: Adam
authors.forename: Petr
authors.initials: P
authors.lastname: Majzlik
authors.forename: Jaromir
authors.initials: J
authors.lastname: Hubalek
authors.forename: Ivo
authors.initials: I
authors.lastname: Provaznik
authors.forename: Rene
authors.initials: R
authors.lastname: Kizek
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood*; D015153:Blotting, Western; D018267:Carcinoma, Acinar Cell; D015331:Cohort Studies; D019075:Electrophoresis, Capillary / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D005978:Glutathione / Q000097:blood; D006801:Humans; D008297:Male; D008668:Metallothionein / Q000097:blood*; D008875:Middle Aged; D011237:Predictive Value of Tests; D017430:Prostate-Specific Antigen / Q000097:blood; D011471:Prostatic Neoplasms / Q000097:blood* / Q000175:diagnosis; D013438:Sulfhydryl Compounds / Q000097:blood
chemical_list: D014408:Biomarkers, Tumor; D013438:Sulfhydryl Compounds; D008668:Metallothionein; D017430:Prostate-Specific Antigen; D005978:Glutathione