title: Major chondroitin sulfate proteoglycans identified in L6J1 myoblast culture.
abstract: The major proteoglycans from L6J1 rat myoblast culture were identified. The proteoglycans were isolated from different constituents of cell culture: culture medium, extracellular matrix (ECM), and myoblasts. To identify their core proteins, the proteoglycans were treated with enzymes specifically digesting chondroitin/dermatan sulfates or chondroitin sulfates. Subsequent electrophoresis and mass spectrometry revealed versican, collagen XII, and inter-α-trypsin inhibitor classified as chondroitin sulfate proteoglycans and biglycan known to be chondroitin/dermatan sulfate proteoglycan. Versican was identified in ECM and the other proteoglycans in the culture medium. Such difference in localization is likely to be a consequence of different biological functions. Versican, collagen XII, and biglycan are synthesized by myoblasts and inter-α-trypsin inhibitor originates from fetal bovine serum (a culture medium component).
authors.affiliation: Department of Cell Cultures, Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia. irinabiochem@mail.ru
authors.forename: I I
authors.initials: II
authors.lastname: Ermakova
authors.forename: G A
authors.initials: GA
authors.lastname: Sakuta
authors.forename: M A
authors.initials: MA
authors.lastname: Potekhina
authors.forename: M A
authors.initials: MA
authors.lastname: Fedorova
authors.forename: R
authors.initials: R
authors.lastname: Hoffmann
authors.forename: V I
authors.initials: VI
authors.lastname: Morozov
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D011508:Chondroitin Sulfate Proteoglycans / Q000032:analysis* / Q000302:isolation & purification; D004586:Electrophoresis; D013058:Mass Spectrometry; D032446:Myoblasts / Q000737:chemistry* / Q000166:cytology; D051381:Rats
chemical_list: D011508:Chondroitin Sulfate Proteoglycans