title: Selective labeling and monitoring pH changes of lysosomes in living cells with fluorogenic pH sensors.
abstract: New BODIPY-based pH probes have been designed with excitation and emission wavelengths suitable for fluorescence microscopy and flow cytometry. These pH probes are cell-permeable, selectively label lysosomes, and can be used for noninvasive monitoring of lysosomal pH changes during physiological and pathological processes.
authors.affiliation: Life Technologies, 29851 Willow Creek Road, Eugene, OR 97402, USA. lai-qiang.ying@lifetech.com
authors.forename: Lai-Qiang
authors.initials: LQ
authors.lastname: Ying
authors.forename: Bruce P
authors.initials: BP
authors.lastname: Branchaud
mesh_terms: D000136:Acid-Base Equilibrium; D000818:Animals; D001896:Boron Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D002417:Cattle; D002460:Cell Line; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008247:Lysosomes / Q000737:chemistry* / Q000378:metabolism*; D018613:Microscopy, Confocal; D008954:Models, Biological; D015394:Molecular Structure
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D005456:Fluorescent Dyes