title: D-allose as ischemic retina injury inhibitor during rabbit vitrectomy.
abstract: OBJECTIVE
  To investigate the protective effects of D-allose, a rare sugar, on pressure-induced ischemia during vitrectomy in the rabbit eye.
  
  
  METHODS
  The rabbits underwent pars plana vitrectomy, and continuous intraocular irrigation at a perfusion pressure of 140 mmHg was performed for 45 min. Intraocular pressure was regulated by adjusting the height of a bottle of balanced saline solution containing D-allose. Morphometric studies were performed to study the effects of D-allose on the histological changes induced by ischemia in the rabbit retina. Electroretinograms (ERGs) were taken before and 1 and 7 days after vitrectomy. Nitroblue tetrazolium was used as an index of superoxide anion (O(2)(·-)) generation. Data were analyzed by use of the unpaired Student's t test.
  
  
  RESULTS
  Seven days after ischemia, significant reductions in both number of ganglion cells and the thickness of the inner plexiform layer were observed. D-Allose significantly inhibited ischemic injury of the inner retina (P < 0.05). On postoperative day 7, amplitudes of ERG b-waves were significantly lower in the control group than in the D-allose group (P < 0.05). D-Allose suppressed the production of O(2)(-).
  
  
  CONCLUSIONS
  Intraocular irrigation with D-allose during vitrectomy may protect the retina against ischemia-induced damage.
authors.affiliation: Department of Ophthalmology, Kagawa Prefectural Central Hospital, 5-4-16 Ban, Takamatu, Kagawa, 760-8557, Japan. m-mizote@chp-kagawa.jp.
authors.forename: Masanori
authors.initials: M
authors.lastname: Mizote
authors.affiliation: Department of Ophthalmology, Kagawa University Faculty of Medicine, 1750-1 Ikenobe, Miki, Kagawa, 761-0793, Japan.
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Hirooka
authors.affiliation: Department of Ophthalmology, Kagawa University Faculty of Medicine, 1750-1 Ikenobe, Miki, Kagawa, 761-0793, Japan.
authors.forename: Kouki
authors.initials: K
authors.lastname: Fukuda
authors.affiliation: Department of Neurobiology, Kagawa University Faculty of Medicine, 1750-1 Ikenobe, Miki, Kagawa, 761-0793, Japan.
authors.forename: Takehiro
authors.initials: T
authors.lastname: Nakamura
authors.affiliation: Department of Neurobiology, Kagawa University Faculty of Medicine, 1750-1 Ikenobe, Miki, Kagawa, 761-0793, Japan.
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Itano
authors.affiliation: Department of Ophthalmology, Kagawa University Faculty of Medicine, 1750-1 Ikenobe, Miki, Kagawa, 761-0793, Japan.
authors.forename: Fumio
authors.initials: F
authors.lastname: Shiraga
mesh_terms: D000818:Animals; D002470:Cell Survival; D004596:Electroretinography; D005260:Female; D005947:Glucose / Q000008:administration & dosage*; D018696:Neuroprotective Agents / Q000008:administration & dosage*; D010100:Oxygen / Q000378:metabolism; D011817:Rabbits; D015427:Reperfusion Injury / Q000503:physiopathology / Q000517:prevention & control*; D012164:Retinal Diseases / Q000503:physiopathology / Q000517:prevention & control*; D012165:Retinal Ganglion Cells / Q000187:drug effects* / Q000378:metabolism; D012171:Retinal Vessels / Q000187:drug effects*; D014821:Vitrectomy
chemical_list: D018696:Neuroprotective Agents; C002055:allose; D005947:Glucose; D010100:Oxygen