title: [Instantaneous expression aFGF-GFP fusion gene in safflower].
abstract: OBJECTIVE
  To study the instantaneous expression aFGF-GFP fusion gene in Carthamus tinctorius.
  
  
  METHODS
  Molecular biology methods were applied to construct aFGF and GFP fusion gene vector, it is transformed into C. tinctorius by Agrobacterium tumefaciens, forming the resistant callus, fluorescence microscopy was used for detection.
  
  
  RESULTS
  aFGF gene and GFP gene were amplified by PCR reaction. It was successfully constructed plant fluorescence expression vector pCAMBIA1390: :35S: :aFGF-GFP, it was used to transform C. tinctorius, and the acquired resistance calli showed strong green fluorescence under UV light.
  
  
  CONCLUSIONS
  The expression of GFP in resistance C. tinrictorius calli is good, it is indicated that aFGF gene in plant cells has also been expressed.
authors.affiliation: Jilin Agricultural University, Ministry of Education Bioreactor and Drug Development Research Center, Jilin 130118, China. xiziyang512@163.com
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.forename: Yongxin
authors.initials: Y
authors.lastname: Guo
authors.forename: Suping
authors.initials: S
authors.lastname: Ren
authors.forename: Tingting
authors.initials: T
authors.lastname: Zhou
authors.forename: Haiyan
authors.initials: H
authors.lastname: Li
authors.forename: Xiaokun
authors.initials: X
authors.lastname: Li
mesh_terms: D031187:Carthamus tinctorius / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D005346:Fibroblast Growth Factors / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D005346:Fibroblast Growth Factors