title: New cytotoxic oxygenated sterols from the marine bryozoan Cryptosula pallasiana.
abstract: Six new sterols (1-6), together with seven known sterols (7-13), were isolated from the CCl(4) extract of the marine bryozoan Cryptosula pallasiana, four (3-6) of which have already been reported as synthetic sterols. This is the first time that these compounds (3-6) are reported as natural sterols. The structures of the new compounds were determined on the basis of the extensive spectroscopic analysis, including two-dimensional (2D) NMR and HR-ESI-MS data. Compounds 1-4, 7 and 10-13 were evaluated for their cytotoxicity against HL-60 human myeloid leukemia cell line, and all of the evaluated compounds exhibited moderate cytotoxicity to HL-60 cells with a range of IC(50) values from 14.73 to 22.11 µg/mL except for compounds 12 and 13.
authors.affiliation: Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, Shannxi, China.|School of Traditional Chinese Medicines, Shenyang Pharmaceutical University, Shenyang 110016, Liaoning, China.
authors.forename: Xiang-Rong
authors.initials: XR
authors.lastname: Tian
authors.affiliation: Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, Shannxi, China.
authors.forename: Hai-Feng
authors.initials: HF
authors.lastname: Tang
authors.affiliation: School of Traditional Chinese Medicines, Shenyang Pharmaceutical University, Shenyang 110016, Liaoning, China.
authors.forename: Yu-Shan
authors.initials: YS
authors.lastname: Li
authors.affiliation: Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200433, China.
authors.forename: Hou-Wen
authors.initials: HW
authors.lastname: Lin
authors.affiliation: Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, Shannxi, China.
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Chen
authors.affiliation: Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, Shannxi, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Ma
authors.affiliation: Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, Shannxi, China.
authors.forename: Min-Na
authors.initials: MN
authors.lastname: Yao
authors.affiliation: Jiangsu Center for Drug Screening & National Drug Screening Laboratory, China Pharmaceutical University, Nanjing 210009, Jiangsu, China.
authors.forename: Ping-Hu
authors.initials: PH
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D002013:Bryozoa / Q000737:chemistry*; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D018922:HL-60 Cells; D006801:Humans; D007951:Leukemia, Myeloid / Q000188:drug therapy; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D013261:Sterols / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D020298:U937 Cells
chemical_list: D000970:Antineoplastic Agents; D013261:Sterols