title: Cloning and characterization of ribosomal protein S29, a deltamethrin resistance associated gene from Culex pipiens pallens.
abstract: The 40S ribosomal protein S29 (RPS29) was shown differentially expressed in deltamethrin (DM)-susceptible and resistant Culex pipiens pallens previously. Here, we cloned RPS29 from this mosquito. An open reading frame (ORF) of 171 bp was found to encode a putative protein with 56 amino acid residues, which shares more than 90% identities with other mosquito RPS29 genes. Moreover, quantitative real-time PCR analysis demonstrated that the transcriptional level of RPS29 was significantly up-regulated both in DM-resistant Cx. p. pallens and C6/36 cells compared with the susceptible ones. Over-expression of RPS29 showed a slight decrease of cell viability in DM-susceptible C6/36 cells under DM treatment and knockdown of RPS29 by siRNA comparably increased cell viability in DM-resistant cells. Our study provides evidence that RPS29 might be associated with fitness cost of DM resistance in mosquitoes for the first time.
authors.affiliation: Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu 210029, People's Republic of China.
authors.forename: Haibo
authors.initials: H
authors.lastname: Sun
authors.forename: Linchun
authors.initials: L
authors.lastname: Sun
authors.forename: Ji
authors.initials: J
authors.lastname: He
authors.forename: Bo
authors.initials: B
authors.lastname: Shen
authors.forename: Jing
authors.initials: J
authors.lastname: Yu
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.forename: Mifang
authors.initials: M
authors.lastname: Yang
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
authors.forename: Donghui
authors.initials: D
authors.lastname: Zhang
authors.forename: Lei
authors.initials: L
authors.lastname: Ma
authors.forename: Changliang
authors.initials: C
authors.lastname: Zhu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003465:Culex / Q000235:genetics* / Q000378:metabolism; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics; D007305:Insecticide Resistance / Q000235:genetics*; D007306:Insecticides / Q000494:pharmacology*; D009570:Nitriles / Q000494:pharmacology*; D011722:Pyrethrins / Q000494:pharmacology*; D034622:RNA Interference; D034741:RNA, Small Interfering; D012269:Ribosomal Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D019476:Insect Proteins; D007306:Insecticides; D009570:Nitriles; D011722:Pyrethrins; D034741:RNA, Small Interfering; D012269:Ribosomal Proteins; C079855:ribosomal protein S29; C017180:decamethrin