title: A fine balance between CCNL1 and TIMP1 contributes to the development of breast cancer cells.
abstract: Cyclin L1 (CCNL1) and tissue inhibitor of matrix metalloproteinase-1 (TIMP1) are candidate genes involved in several types of cancer. However, the expression of CCNL1 and the relationship between CCNL1 and TIMP1 in breast cancer cells is unknown. Using patients' breast cancer tissues, the expression of CCNL1 and TIMP1 was measured by cDNA microarray and further confirmed by real-time RT-PCR and western blotting. Overexpression or repression of CCNL1 and TIMP1, individually or together, was performed in breast cancer MDA-MB-231 cells by transient transformation methods to investigate their role in breast cancer cell growth. Simultaneously, mRNA and protein expression levels of CCNL1 and TIMP1 were also measured. CCNL1 and TIMP1 expression was significantly elevated in breast cancer tissues compared with that in peri-breast cancer tissues of patients by cDNA microarray and these results were further confirmed by real-time RT-PCR and western blotting. Interestingly, in vitro experiments showed a stimulatory effect of TIMP1 and an inhibitory effect of CCNL1 on growth of MDA-MB-231 cells. Co-expression or co-repression of these two genes did not affect cell growth. Overexpression of CCNL1 and TIMP1 individually induced overexpression of each other. These data demonstrate that there is a fine balance between CCNL1 and TIMP1, which may contribute to breast cancer development.
authors.affiliation: College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun 130062, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Peng
authors.forename: Ma
authors.initials: M
authors.lastname: Yanjiao
authors.forename: Wang
authors.initials: W
authors.lastname: Ai-guo
authors.forename: Gong
authors.initials: G
authors.lastname: Pengtao
authors.forename: Li
authors.initials: L
authors.lastname: Jianhua
authors.forename: Yang
authors.initials: Y
authors.lastname: Ju
authors.forename: Ouyang
authors.initials: O
authors.lastname: Hongsheng
authors.forename: Zhang
authors.initials: Z
authors.lastname: Xichen
mesh_terms: D000328:Adult; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology*; D049109:Cell Proliferation; D016213:Cyclins / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000235:genetics*; D014407:Tumor Cells, Cultured
chemical_list: C468873:CCNL1 protein, human; D016213:Cyclins; D019715:Tissue Inhibitor of Metalloproteinase-1