title: Yeast dynamics during the fermentation of brined green olives treated in the field with kaolin and Bordeaux mixture to control the olive fruit fly.
abstract: The yeast microbiota associated with naturally fermented and inoculated green table olives, differently treated in the field with non-conventional repellent and antiovipositional products in the control of Bactrocera oleae, was analysed using a combination of culture-dependent and -independent molecular fingerprinting. The routine yeast isolation gave rise to 118 strains, whose identification was performed by PCR-RFLP of the internal transcribed spacer (ITS) regions. Total DNA was extracted directly from the brine throughout fermentation by means of an experimental protocol that included the removal of Taq polymerase inhibitors. Denaturing Gradient Gel Electrophoresis (DGGE) of 26S rRNA gene PCR amplicons highlighted the yeast community. Comparison of both culture-dependent and independent methods indicated that the yeast species Saccharomyces cerevisiae, Wickerhamomyces anomalus, Candida diddensiae and Issatchenkia orientalis were dominant during fermentation despite the addition of the Lactobacillus plantarum starter used in brining. The resultant isolated species were unaffected by treatments in field, except for C. diddensiae whose growth was delayed by kaolin.
authors.affiliation: DISPA, Sezione di Tecnologia e Microbiologia degli Alimenti, University of Catania, Italy.
authors.forename: Serena
authors.initials: S
authors.lastname: Muccilli
authors.forename: Cinzia
authors.initials: C
authors.lastname: Caggia
authors.forename: Cinzia L
authors.initials: CL
authors.lastname: Randazzo
authors.forename: Cristina
authors.initials: C
authors.lastname: Restuccia
mesh_terms: D058645:Denaturing Gradient Gel Electrophoresis / Q000379:methods; D005285:Fermentation; D007300:Insect Control; D007616:Kaolin; D048191:Lactiplantibacillus plantarum; D031658:Olea / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods; D012492:Salts; D015003:Yeasts / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*
chemical_list: D012492:Salts; C017082:brine; D007616:Kaolin