title: Problem-solving test: Real-time polymerase chain reaction.
abstract: Terms to be familiar with before you start to solve the test: polymerase chain reaction, DNA amplification, electrophoresis, breast cancer, HER2 gene, genomic DNA, in vitro DNA synthesis, template, primer, Taq polymerase, 5'→3' elongation activity, 5'→3' exonuclease activity, deoxyribonucleoside triphosphates, DNA structure, proofreading, thermocycler, fluorescence.
authors.affiliation: Department of Medical Biology, Medical School, University of Pécs, H-7624 Pécs, Hungary. jozsef.szeberenyi@aok.pte.hu.
authors.forename: József
authors.initials: J
authors.lastname: Szeberényi