title: Prognostic significance of tumor/stromal caveolin-1 expression in breast cancer patients.
abstract: Caveolin-1 (Cav-1) has been extensively characterized in cancer biological research. However, the role of Cav-1 in the interaction between tumor and stromal cells remains unclear. In the present study, we examined Cav-1 expression in tumor cells and stromal cells in breast cancer tissue by immunohistochemical analysis and evaluated its prognostic value in a training cohort. Immunohistochemical analysis of Cav-1 expression was scored as (++), (+) or (-) according to the proportion of positively stained tumor cells (T) and stromal cells (S). Correlation analysis between tumor/stromal Cav-1 expression and clinicopathological parameters revealed that only T(++) Cav-1 status was positively associated with tumor size and histological nodal status (P = 0.019 and 0.021, respectively). Univariate analysis revealed that combined T(++)/S(-) status was significantly correlated with unfavorable prognostic outcomes (P < 0.001). Multivariate analysis demonstrated that this combined status is an independent prognostic factor for primary breast cancer (P = 0.002). Clinical outcomes in different subgroups of breast cancer patients were also strictly dependent on this combined status (P < 0.05). The prognostic value of T(++)/S(-) Cav-1 status was also validated in the testing cohort. Collectively, our data indicate that high Cav-1 expression in tumor cells and lack of this expression in stromal cells could help identify a particular subgroup of breast cancer patients with potentially poor survival. Further studies are required to understand the regulatory mechanism of Cav-1 in the tumor microenvironment.
authors.affiliation: Department of Breast Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
authors.forename: Niansong
authors.initials: N
authors.lastname: Qian
authors.forename: Takayuki
authors.initials: T
authors.lastname: Ueno
authors.forename: Nobuko
authors.initials: N
authors.lastname: Kawaguchi-Sakita
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kawashima
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Yoshida
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Mikami
authors.forename: Tomoko
authors.initials: T
authors.lastname: Wakasa
authors.forename: Masayuki
authors.initials: M
authors.lastname: Shintaku
authors.forename: Shigeru
authors.initials: S
authors.lastname: Tsuyuki
authors.forename: Takashi
authors.initials: T
authors.lastname: Inamoto
authors.forename: Masakazu
authors.initials: M
authors.lastname: Toi
mesh_terms: D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000737:chemistry / Q000401:mortality* / Q000473:pathology; D051242:Caveolin 1 / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D015331:Cohort Studies; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D011379:Prognosis
chemical_list: C495540:CAV1 protein, human; D051242:Caveolin 1