title: Liquid chromatography/tandem mass spectrometry of dolichols and polyprenols, lipid sugar carriers across evolution.
abstract: Across evolution, dolichols and polyprenols serve as sugar carriers in biosynthetic processes that include protein glycosylation and lipopolysaccharide biogenesis. Liquid chromatography coupled with electrospray ionization mass spectrometry offers a powerful tool for studying dolichols and polyprenols in their alcohol or glycan-modified forms in members of all three domains of life. In the following, recent examples of the how different versions of this analytical approach, namely reverse phase liquid chromatography-multiple reaction monitoring, normal phase liquid chromatography/tandem mass spectrometry and normal phase liquid chromatography-precursor ion scan detection have respectively served to address novel aspects of dolichol or polyprenol biology in Eukarya, Archaea and Bacteria.
authors.affiliation: Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA. zguan@biochem.duke.edu
authors.forename: Ziqiang
authors.initials: Z
authors.lastname: Guan
authors.forename: Jerry
authors.initials: J
authors.lastname: Eichler
mesh_terms: D000818:Animals; D001419:Bacteria / Q000378:metabolism; D005075:Biological Evolution; D002241:Carbohydrates / Q000032:analysis*; D002853:Chromatography, Liquid; D004286:Dolichols / Q000032:analysis* / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D002241:Carbohydrates; D004286:Dolichols