title: Influence of insulin-like growth factor-I antiserum to proliferation of T-cell receptor-activated human T-lymphocytes.
abstract: We have previously reported that Insulin-like growth factor (IGF-I) is a cellular growth factor of human transformed T-lymphocytes. To evaluate the role of IGF-I for nontransformed T-cells we investigated synthesis of IGF-I and the influence of an IGF-I antiserum, UBK487, during proliferation process. IGF-I is synthesized by B-lymphocytes and monocytes during proliferation. Via the T-cell receptor (CD3 subunit) induced proliferation of resting T-cells was inhibited by addition of UBK487. The addition of monocytes to the resting T-cell fraction reversed the UBK487 effect. Using UBK487, a 12 kDa protein could be immunoprecipitated from unstimulated or PHA stimulated T-cells whereas a 36 kDa protein could be immunoprecipitated from OKT3 stimulated T-cells.
authors.affiliation: DEPT PEDIAT,FRANKFURT,GERMANY. UNIV CATANIA,DEPT ENDOCRINOL,I-95124 CATANIA,ITALY. UNIV HEIDELBERG,DEPT PEDIAT ENDOCRINOL,W-6900 HEIDELBERG,GERMANY. UNIV HEIDELBERG,CANC RES CTR,W-6900 HEIDELBERG,GERMANY.
authors.forename: K
authors.initials: K
authors.lastname: Hartmann
authors.forename: R
authors.initials: R
authors.lastname: Teckentrup
authors.forename: D
authors.initials: D
authors.lastname: Nixdorf
authors.forename: S
authors.initials: S
authors.lastname: Meuer
authors.forename: V
authors.initials: V
authors.lastname: Papa
authors.forename: U
authors.initials: U
authors.lastname: Heinrich