title: Atomic model of CPV reveals the mechanism used by this single-shelled virus to economically carry out functions conserved in multishelled reoviruses.
abstract: Unlike the multishelled viruses in the Reoviridae, cytoplasmic polyhedrosis virus (CPV) is single shelled, yet stable and fully capable of carrying out functions conserved within Reoviridae. Here, we report a 3.1 Å resolution cryo electron microscopy structure of CPV and derive its atomic model, consisting of 60 turret proteins (TPs), 120 each of capsid shell proteins (CSPs) and large protrusion proteins (LPPs). Two unique segments of CSP contribute to CPV's stability: an inserted protrusion domain interacting with neighboring proteins, and an N-anchor tying up CSPs together through strong interactions such as β sheet augmentation. Without the need to interact with outer shell proteins, LPP retains only the N-terminal two-third region containing a conserved helix-barrel core and interacts exclusively with CSP. TP is also simplified, containing only domains involved in RNA capping. Our results illustrate how CPV proteins have evolved in a coordinative manner to economically carry out their conserved functions.
authors.affiliation: Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA 90095-7364, USA.
authors.forename: Xuekui
authors.initials: X
authors.lastname: Yu
authors.forename: Peng
authors.initials: P
authors.lastname: Ge
authors.forename: Jiansen
authors.initials: J
authors.lastname: Jiang
authors.forename: Ivo
authors.initials: I
authors.lastname: Atanasov
authors.forename: Z Hong
authors.initials: ZH
authors.lastname: Zhou
mesh_terms: D000595:Amino Acid Sequence; D002213:Capsid / Q000737:chemistry* / Q000378:metabolism; D036022:Capsid Proteins / Q000737:chemistry* / Q000378:metabolism; D020285:Cryoelectron Microscopy; D019143:Evolution, Molecular; D007091:Image Processing, Computer-Assisted; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D055550:Protein Stability; D012367:RNA, Viral / Q000378:metabolism*; D012087:Reoviridae / Q000737:chemistry / Q000378:metabolism; D012088:Reoviridae Infections / Q000821:virology
chemical_list: D036022:Capsid Proteins; D012367:RNA, Viral