title: Tangential migration and proliferation of intermediate progenitors of GABAergic neurons in the mouse telencephalon.
abstract: In the embryonic neocortex, neuronal precursors are generated in the ventricular zone (VZ) and accumulate in the cortical plate. Recently, the subventricular zone (SVZ) of the embryonic neocortex was recognized as an additional neurogenic site for both principal excitatory neurons and GABAergic inhibitory neurons. To gain insight into the neurogenesis of GABAergic neurons in the SVZ, we investigated the characteristics of intermediate progenitors of GABAergic neurons (IPGNs) in mouse neocortex by immunohistochemistry, immunocytochemistry, single-cell RT-PCR and single-cell array analysis. IPGNs were identified by their expression of some neuronal and cell cycle markers. Moreover, we investigated the origins of the neocortical IPGNs by Cre-loxP fate mapping in transgenic mice and the transduction of part of the telencephalic VZ by Cre-reporter plasmids, and found them in the medial and lateral ganglionic eminence. Therefore, they must migrate tangentially within the telencephalon to reach the neocortex. Cell-lineage analysis by simple-retrovirus transduction revealed that the neocortical IPGNs self-renew and give rise to a small number of neocortical GABAergic neurons and to a large number of granule and periglomerular cells in the olfactory bulb. IPGNs are maintained in the neocortex and may act as progenitors for adult neurogenesis.
authors.affiliation: Department of Morphological Neural Science, Graduate School of Medical Sciences, Kumamoto University, Kumamoto 860-8556 Japan.
authors.forename: Shengxi
authors.initials: S
authors.lastname: Wu
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Esumi
authors.forename: Keisuke
authors.initials: K
authors.lastname: Watanabe
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.forename: Kouichi C
authors.initials: KC
authors.lastname: Nakamura
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Nakamura
authors.forename: Kouhei
authors.initials: K
authors.lastname: Kometani
authors.forename: Nagahiro
authors.initials: N
authors.lastname: Minato
authors.forename: Yuchio
authors.initials: Y
authors.lastname: Yanagawa
authors.forename: Kaori
authors.initials: K
authors.lastname: Akashi
authors.forename: Kenji
authors.initials: K
authors.lastname: Sakimura
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kaneko
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Tamamaki
mesh_terms: D000818:Animals; D002465:Cell Movement; D049109:Cell Proliferation; D051379:Mice; D019579:Neocortex / Q000166:cytology / Q000196:embryology; D058953:Neural Stem Cells / Q000166:cytology*; D055495:Neurogenesis; D009474:Neurons / Q000166:cytology*; D009830:Olfactory Bulb / Q000166:cytology / Q000196:embryology; D013687:Telencephalon / Q000166:cytology* / Q000196:embryology; D005680:gamma-Aminobutyric Acid
chemical_list: D005680:gamma-Aminobutyric Acid