title: Visualizing the effects of biofilm structures on the influx of fluorescent material using combined confocal reflection and fluorescent microscopy.
abstract: Mass transport into the interior of biofilms and biological aggregates is a critical factor that affects their metabolic activity. In this study, we demonstrated the utility of a simple procedure that combines confocal reflection microscopy and fluorescent confocal laser scanning microscopy to visually explain the effects of biofilm structure on mass transport.
authors.affiliation: Institute of Life and Environmental Sciences, University of Tsukuba, Tennnoudai 1–1–1, Tsukuba, Ibaraki, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Yawata
authors.forename: Hiroo
authors.initials: H
authors.lastname: Uchiyama
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Nomura
mesh_terms: D018441:Biofilms / Q000254:growth & development*; D005456:Fluorescent Dyes / Q000378:metabolism*; D008856:Microscopy, Fluorescence / Q000379:methods; D011550:Pseudomonas aeruginosa / Q000378:metabolism / Q000502:physiology*
chemical_list: D005456:Fluorescent Dyes