title: Enhanced expression of C-myc gene and binding-protein to the 2.3-kb upstream region of the p1 promoter in human liver and colon cancers.
abstract: We investigated binding proteins in the upstream regulatory region of c-myc gene in human liver and colon cancers. Gel shift assay revealed a nuclear protein which bound to the HindIII/PstI fragment (nt.-2328 to nt.-2118) upstream of the pi promoter of c-myc gene. DNase I footprinting showed a unique 25bp binding sequence (nt.-2305 to nt.-2281), not related to any reported elements. The amount of binding protein was higher in the cancerous than in the non-cancerous tissues. This suggests that the 25bp sequence possibly functions as a cis-acting positive element of the c-myc gene expression.
authors.affiliation: CANC INST HOSP,JFCR,DEPT GENE RES,TOKYO 170,JAPAN. CHIBA UNIV,SCH MED,DEPT SURG,CHIBA 260,JAPAN.
authors.forename: K
authors.initials: K
authors.lastname: Saigo
authors.forename: K
authors.initials: K
authors.lastname: Yaginuma
authors.forename: H
authors.initials: H
authors.lastname: Hayashi
authors.forename: K
authors.initials: K
authors.lastname: Isono
authors.forename: S
authors.initials: S
authors.lastname: Takada
authors.forename: K
authors.initials: K
authors.lastname: Koike