title: Heat shock protein DnaK--substrate of actin-specific bacterial protease ECP32.
abstract: It has been found that actin-specific bacterial protease ECP32 cleaves prokaryotic heat shock protein DnaK, which belongs to the family of heat shock proteins with molecular weight 70 kDa. We propose a new one-step method for DnaK purification using heat treatment. The technique yields ~1 mg of partially purified DnaK from 25 g of wet bacterial biomass. Polyclonal antibodies against DnaK were obtained. The degree of ECP32 catalyzed proteolysis of partially purified DnaK and that of DnaK in initial cell extracts was compared.
authors.affiliation: Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia. avmoro@gmail.com
authors.forename: A V
authors.initials: AV
authors.lastname: Morozova
authors.forename: S Yu
authors.initials: SY
authors.lastname: Khaitlina
authors.forename: A Yu
authors.initials: AY
authors.lastname: Malinin
mesh_terms: D000199:Actins / Q000737:chemistry*; D001412:Bacillus subtilis / Q000201:enzymology; D002176:Candida albicans / Q000201:enzymology; D010450:Endopeptidases / Q000737:chemistry*; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D006090:Gram-Negative Bacteria / Q000201:enzymology; D018840:HSP70 Heat-Shock Proteins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D007106:Immune Sera; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D020539:Sequence Analysis, Protein
chemical_list: D000199:Actins; D029968:Escherichia coli Proteins; D018840:HSP70 Heat-Shock Proteins; D007106:Immune Sera; D010450:Endopeptidases; C097881:proteinase ECP 32; C063842:dnaK protein, E coli