title: Determination of anthocyanins in cranberry fruit and cranberry fruit products by high-performance liquid chromatography with ultraviolet detection: single-laboratory validation.
abstract: A single-laboratory validation study was conducted on an HPLC method for the detection and quantification of cyanidin-3-O-galactoside (C3Ga), cyanidin-3-O-glucoside (C3GI), cyanidin-3-O-arabinoside (C3Ar), peonidin-3-O-galactoside (P3Ga), and peonidin-3-O-arabinoside (P3Ar) in cranberry fruit (Vaccinium macrocarpon Aiton) raw material and finished products. An extraction procedure using a combination of sonication and shaking with acidified methanol was optimized for all five anthocyanins in freeze-dried cranberry fruit and finished products (commercial extract powder, juice, and juice cocktail). Final extract solutions were analyzed by HPLC using a C18 RP column. Calibration curves for all anthocyanin concentrations had correlation coefficients (r2) of > or = 99.8%. The method detection limits for C3Ga, C3Gl, C3Ar, P3Ga, and P3Ar were estimated to be 0.018, 0.016, 0.006, 0.013, and 0.011 microg/mL, respectively. Separation was achieved with a chromatographic run time of 35 min using a binary mobile phase with gradient elution. Quantitative determination performed in triplicate on four test materials on each of 3 days (n = 12) resulted in RSD(r) from 1.77 to 3.31%. Analytical range, as defined by the calibration curves, was 0.57-36.53 microg/mL for C3Ga, 0.15-9.83 microg/mL for C3GI, 0.28-17.67 microg/mL for C3Ar, 1.01-64.71 microg/mL for P3Ga, and 0.42-27.14 microg/mL for P3Ar. For solid materials prepared by the described method, this translates to 0.06-3.65 mglg for C3Ga, 0.02-0.98 mg/g for C3Gl, 0.03-1.77 mg/g for C3Ar, 0.10-6.47 mg/g for P3Ga, and 0.04-2.71 mg/g for P3Ar.
authors.affiliation: University of British Columbia Okanagan, 3333 University Way, Kelowna, British Columbia, Canada V1V 1V7. paula_brown@bcit.ca
authors.forename: Paula N
authors.initials: PN
authors.lastname: Brown
authors.forename: Paul R
authors.initials: PR
authors.lastname: Shipley
mesh_terms: D000872:Anthocyanins / Q000737:chemistry*; D001628:Beverages / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005638:Fruit / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D014466:Ultraviolet Rays; D029799:Vaccinium macrocarpon / Q000737:chemistry*
chemical_list: D000872:Anthocyanins; D010936:Plant Extracts