title: Human nm23-h1-protein and h2-protein have similar nucleoside diphosphate kinase-activities.
abstract: The nucleoside diphosphate kinase activity of nm23-H1 and nm23-H2 proteins was examined. Full length nm23-H1 and nm23-H2 proteins were produced in E.coli in fusion form with a 26 kDa glutathione S-transferase (GST). Affinity purified nm23-H1 and nm23-H2 formed phosphoenzyme intermediates when incubated with [gamma-P-32]ATP. The formation of GTP from GDP was also demonstrated by these two proteins by thin layer chromatography. The 26 kDa GST alone did not show similar activity. Both nm23-H1 and nm23-H2 shared very similar biochemical characteristics, namely, time kinetics, pH, temperature and cation dependency for the formation of the phosphoenzyme intermediates.
authors.affiliation: NAGASAKI UNIV,SCH MED,DEPT ONCOL,12-4 SAKAMOTO MACHI,NAGASAKI 852,JAPAN. KANAZAWA UNIV,SCH MED,DEPT SURG 2,KANAZAWA,ISHIKAWA 920,JAPAN.
authors.forename: T
authors.initials: T
authors.lastname: Urano
authors.forename: S
authors.initials: S
authors.lastname: Fushida
authors.forename: K
authors.initials: K
authors.lastname: Furukawa
authors.forename: H
authors.initials: H
authors.lastname: Shiku