title: Normal and abnormal p21 h-ras posttranslational products in human neoplastic and nonneoplastic liver-tissues.
abstract: Using two-dimensional Western blot analysis with a pan-ras antibody, we previously defined conditions that allow to resolve the four post-translational p21-H-ras products expressed in normal mature rat tissues. Using the same approach, we conducted experiments that sometimes revealed deviations from the normal basal p21-H-ras pattern in primary human liver tumors. One type of alteration encountered was indicative of modifications in the relative rate of accomplishment of the different steps in the post-translational metabolisation of the protein, resulting in the accumulation of precursors of the fully-processed p21-H-ras product. This was also observed during ontogenesis and might thus be correlated with either cellular growth potential or differentiation. The second type of altered pattern is defined by the detection of abnormal spots and probably corresponds to the presence of mutant p21-ras products.
authors.affiliation: UNIV LIBRE BRUXELLES,SCH MED,IRIBHN,DEPT BIOL,B-1070 BRUSSELS,BELGIUM. UNIV LIBRE BRUXELLES,HOP ERASME,DEPT CLIN CHEM,B-1070 BRUSSELS,BELGIUM. UNIV LIBRE BRUXELLES,HOP ERASME,DEPT GASTROENTEROL,B-1070 BRUSSELS,BELGIUM.
authors.forename: B
authors.initials: B
authors.lastname: Gulbis
authors.forename: K
authors.initials: K
authors.lastname: Alexandre
authors.forename: M
authors.initials: M
authors.lastname: Delhaye
authors.forename: P
authors.initials: P
authors.lastname: Galand