title: Disulfide-stabilized helical hairpin structure and activity of a novel antifungal peptide EcAMP1 from seeds of barnyard grass (Echinochloa crus-galli).
abstract: This study presents purification, activity characterization, and (1)H NMR study of the novel antifungal peptide EcAMP1 from kernels of barnyard grass Echinochloa crus-galli. The peptide adopts a disulfide-stabilized α-helical hairpin structure in aqueous solution and thus represents a novel fold among naturally occurring antimicrobial peptides. Micromolar concentrations of EcAMP1 were shown to inhibit growth of several fungal phytopathogens. Confocal microscopy revealed intensive EcAMP1 binding to the surface of fungal conidia followed by internalization and accumulation in the cytoplasm without disturbance of membrane integrity. Close spatial structure similarity between EcAMP1, the trypsin inhibitor VhTI from seeds of Veronica hederifolia, and some scorpion and cone snail toxins suggests natural elaboration of different functions on a common fold.
authors.affiliation: M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, 117997 Moscow, Russian Federation.
authors.forename: Svetlana B
authors.initials: SB
authors.lastname: Nolde
authors.forename: Alexander A
authors.initials: AA
authors.lastname: Vassilevski
authors.forename: Eugene A
authors.initials: EA
authors.lastname: Rogozhin
authors.forename: Nikolay A
authors.initials: NA
authors.lastname: Barinov
authors.forename: Tamara A
authors.initials: TA
authors.lastname: Balashova
authors.forename: Olga V
authors.initials: OV
authors.lastname: Samsonova
authors.forename: Yuri V
authors.initials: YV
authors.lastname: Baranov
authors.forename: Alexey V
authors.initials: AV
authors.lastname: Feofanov
authors.forename: Tsezi A
authors.initials: TA
authors.lastname: Egorov
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Arseniev
authors.forename: Eugene V
authors.initials: EV
authors.lastname: Grishin
mesh_terms: D000935:Antifungal Agents / Q000737:chemistry* / Q000494:pharmacology; D031747:Echinochloa / Q000737:chemistry*; D005658:Fungi / Q000254:growth & development; D010455:Peptides / Q000737:chemistry* / Q000494:pharmacology; D010935:Plant Diseases / Q000382:microbiology; D010940:Plant Proteins / Q000737:chemistry* / Q000494:pharmacology; D017433:Protein Structure, Secondary
chemical_list: D000935:Antifungal Agents; D010455:Peptides; D010940:Plant Proteins