title: Cell-specific visualization of jasmonates in wounded tomato and Arabidopsis leaves using jasmonate-specific antibodies.
abstract: Jasmonates are well-characterized signals in the development of plants and their response to abiotic and biotic stresses, such as touch and wounding by herbivores. A gap in our knowledge on jasmonate-induced processes, however, is the cellular localization of jasmonates. Here, a novel antibody-based approach was developed to visualize jasmonates in cross-sections of plant material. Antibodies raised in rabbits against BSA-coupled jasmonic acid (JA) are specific for JA, its methyl ester and isoleucine conjugate. They do not bind to 12-oxophytodienoic acid, 12-hydoxy-JA or coronatine. These antibodies were used in combination with newly established fixation and embedding methods. Jasmonates were rapidly and uniformly distributed within all cells near the site of damage of a mechanically wounded tomato (Solanum lycopersicum) leaf. Leaf tissue distally located to the wound site exhibited identical distribution, but had a lower signal intensity. The occurrence of jasmonates in all cell types of a wounded leaf was accompanied by transcript accumulation of early JA-induced genes visualized by in situ hybridization. With these new antibodies, a powerful tool is available to detect cell-specifically the occurrence of jasmonates in any jasmonate-dependent stress response or developmental process of plants.
authors.affiliation: Leibniz-Institut für Pflanzenbiochemie, D-06120 Halle, Germany.
authors.forename: Kati
authors.initials: K
authors.lastname: Mielke
authors.affiliation: Leibniz-Institut für Pflanzenbiochemie, D-06120 Halle, Germany.
authors.forename: Susanne
authors.initials: S
authors.lastname: Forner
authors.affiliation: Leibniz-Institut für Pflanzenbiochemie, D-06120 Halle, Germany.
authors.forename: Robert
authors.initials: R
authors.lastname: Kramell
authors.affiliation: Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK), D-06466 Gatersleben, Germany.
authors.forename: Udo
authors.initials: U
authors.lastname: Conrad
authors.affiliation: Leibniz-Institut für Pflanzenbiochemie, D-06120 Halle, Germany.
authors.forename: Bettina
authors.initials: B
authors.lastname: Hause
mesh_terms: D000906:Antibodies / Q000378:metabolism; D017360:Arabidopsis / Q000166:cytology* / Q000378:metabolism; D003517:Cyclopentanes / Q000032:analysis*; D005231:Fatty Acids, Unsaturated / Q000032:analysis; D007150:Immunohistochemistry / Q000379:methods*; D018551:Solanum lycopersicum / Q000166:cytology* / Q000378:metabolism; D054883:Oxylipins / Q000032:analysis*; D010937:Plant Growth Regulators / Q000032:analysis; D018515:Plant Leaves / Q000166:cytology* / Q000378:metabolism
chemical_list: D000906:Antibodies; D003517:Cyclopentanes; D005231:Fatty Acids, Unsaturated; D054883:Oxylipins; D010937:Plant Growth Regulators; C025999:12-oxophytodienoic acid; C011006:jasmonic acid