title: Early changes of LIFR and gp130 in sciatic nerve and muscle of diabetic mice.
abstract: Peripheral neuropathy is a common complication of diabetes mediated by alterations of growth factors. Members of the neuropoietic cytokine family, which include IL-6, LIF, and CNTF among others, have been shown to be important regulators of peripheral nerves and the muscles that they innervate. To investigate their potential role in diabetic nerve and muscle, we studied the expression of the shared receptor subunits, LIFR and gp130 in a mouse model of streptozotocin (STZ)-induced diabetes. The results of Western blotting and densitometric analysis showed that both LIFR and gp130 protein expression were increased in diabetic sciatic nerve compared to control mice at early time points following STZ injection. In diabetic gastrocnemius muscle, LIFR and gp130 were increased from 3 days to 24 weeks following STZ injection. In contrast, both LIFR and gp130 protein expression were decreased in diabetic soleus muscle at 3-days post-injection. Our results suggest that hyperglycemia results in changes to nerve and muscle soon after the onset of diabetes and that cytokines may play a role in this process.
authors.affiliation: Department of Biology, California State University, Northridge, 91330-8330, USA.
authors.forename: Claudia M
authors.initials: CM
authors.lastname: Toledo-Corral
authors.forename: Lisa R
authors.initials: LR
authors.lastname: Banner
mesh_terms: D000367:Age Factors; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D015153:Blotting, Western; D050822:Cytokine Receptor gp130 / Q000235:genetics / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D005260:Female; D053680:Leukemia Inhibitory Factor Receptor alpha Subunit / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018482:Muscle, Skeletal / Q000378:metabolism*; D012584:Sciatic Nerve / Q000378:metabolism*; D013311:Streptozocin; D015854:Up-Regulation
chemical_list: D001786:Blood Glucose; C494200:Il6st protein, mouse; D053680:Leukemia Inhibitory Factor Receptor alpha Subunit; C507841:Lifr protein, mouse; D050822:Cytokine Receptor gp130; D013311:Streptozocin