title: Maturation of bone marrow-derived dendritic cells by a novel β-glucan purified from Paenibacillus polymyxa JB115.
abstract: We investigated the immunostimulatory effects of a novel β-glucan purified from Paenibacillus (P.) polymyxa JB115 on bone marrow-derived dendritic cells (DCs), a type of potent antigen-presenting cells. β-glucan isolated from P. polymyxa JB115 enhanced the viability and induced the maturation of DCs. β-glucan markedly increased the cytokine production of DCs and surface expression of DC markers. In addition, DCs treated with β-glucan showed a higher capacity to stimulate allogeneic spleen cell proliferation compared to those treated with medium alone. These results demonstrate the effect of β-glucan on DC maturation and may increase the use of β-glucan.
authors.affiliation: College of Veterinary Medicine, Jeju National University, Jeju 690-756, Korea.
authors.forename: Eun-Ju
authors.initials: EJ
authors.lastname: Ko
authors.forename: Yun-Young
authors.initials: YY
authors.lastname: Byon
authors.forename: Youngheun
authors.initials: Y
authors.lastname: Jee
authors.forename: Taekyun
authors.initials: T
authors.lastname: Shin
authors.forename: Seung-Chun
authors.initials: SC
authors.lastname: Park
authors.forename: Tae-Wook
authors.initials: TW
authors.lastname: Hahn
authors.forename: Hong-Gu
authors.initials: HG
authors.lastname: Joo
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects* / Q000276:immunology*; D002470:Cell Survival / Q000187:drug effects / Q000276:immunology*; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects* / Q000276:immunology*; D005434:Flow Cytometry; D016130:Immunophenotyping / Q000379:methods; D018664:Interleukin-12 / Q000032:analysis / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000032:analysis / Q000276:immunology; D056507:Paenibacillus / Q000737:chemistry*; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000276:immunology; D047071:beta-Glucans / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D014409:Tumor Necrosis Factor-alpha; D047071:beta-Glucans; D018664:Interleukin-12; D009569:Nitric Oxide