title: Semi-quantitative differences in gene transcription profiles between sexes of a marine snail by a new variant of cDNA-AFLP analysis.
abstract: A variant of the cDNA-AFLP method coupled to an automated sequencer was used to quantify transcripts differentially expressed between sexes of the marine snail Littorina saxatilis. First, we conducted a validation study of the technique using known concentrations of a commercial marker. Second, we analysed six replicates of males and females from a population showing no apparent sexual dimorphism. The results confirm that the method can be properly used within the range of DNA concentrations utilized. In addition, we detected a small percentage of spots (1.8%) differentially expressed between sexes, as expected from a low to moderately sexual dimorphic species.
authors.affiliation: Departamento de Bioquímica, Genética e Inmunología, Facultad de Biología, Campus As Lagoas-Marcosende Universidad de Vigo, 36310 Vigo, Spain Department of Biology, Pavillon Charles-Eugène-Marchand, 1030 Avenue de la Médecine, Université Laval, Québec, QC, Canada G1V 0A6.
authors.forename: M
authors.initials: M
authors.lastname: Martínez-Fernández
authors.forename: L
authors.initials: L
authors.lastname: Bernatchez
authors.forename: E
authors.initials: E
authors.lastname: Rolán-Alvarez
authors.forename: H
authors.initials: H
authors.lastname: Quesada