title: STR markers for detecting heterogeneity in Indian population.
abstract: Short tandem repeats are highly polymorphic sequences of nucleotides, which are abundant in eukaryotic genome. They form approximately 3% of the total human genome and occur on average in every 10, 000 nucleotides. Due to their small dimension, low mutation, and high level of polymorphism, these markers are intensely used as important genetic markers for mapping studies, disease diagnosis, and human identity testing. In the present study allelic distribution of four autosomal short tandem repeat markers (D21S2055, D21S11, D21S1435 and D21S1411) has been analyzed in Indian population. For determination of heterogeneity and their allelic frequency QF-PCR analysis have been done. All the loci were found highly polymorphic. Marker D21S1411 was the most informative (93.6%) and D21S1435 (70.1%) was the least informative marker in Indian population.
authors.affiliation: Department of Medical Genetics, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Raibareilly Road, Lucknow, 226014, India.
authors.forename: Shalu
authors.initials: S
authors.lastname: Jain
authors.forename: Inusha
authors.initials: I
authors.lastname: Panigrahi
authors.forename: Jayesh
authors.initials: J
authors.lastname: Sheth
authors.forename: Sarita
authors.initials: S
authors.lastname: Agarwal
mesh_terms: D017931:DNA Primers / Q000235:genetics; D005453:Fluorescence; D005787:Gene Frequency; D018740:Genetic Heterogeneity; D005819:Genetic Markers / Q000235:genetics*; D005838:Genotype; D006801:Humans; D007194:India; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods
chemical_list: D017931:DNA Primers; D005819:Genetic Markers