title: Sphingobium jiangsuense sp. nov., a 3-phenoxybenzoic acid-degrading bacterium isolated from a wastewater treatment system.
abstract: A non-sporulating, non-motile, catalase- and oxidase-positive, Gram-negative, rod-shaped bacterial strain, designated BA-3T, was isolated from activated sludge of a wastewater treatment facility. The strain was able to degrade about 95 % of 100 mg 3-phenoxybenzoic acid l(-1) within 2 days of incubation. Growth occurred in the presence of 0-2 % (w/v) NaCl [optimum, 0.5 % (w/v) NaCl], at pH 5.5-9.0 (optimum, pH 7.0) and at 10-37 °C (optimum, 28 °C). Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain BA-3T was a member of the genus Sphingobium; it showed highest gene sequence similarity to Sphingobium qiguonii X23T (98.2 %), and similarities of <97.0 % with strains of other Sphingobium species. The polar lipid pattern, the presence of spermidine and ubiquinone Q-10, the predominance of summed feature 8 (C18:1ω6c and/or C18:1ω7c) in the cellular fatty acid profile and the DNA G+C content also supported affiliation of the isolate to the genus Sphingobium. Strain BA-3T showed low DNA-DNA relatedness values (21.3±0.8 %) with Sphingobium qiguonii X23(T). Based on phenotypic, genotypic and phylogenetic data, strain BA-3T represents a novel species of the genus Sphingobium, for which the name Sphingobium jiangsuense sp. nov. is proposed; the type strain is BA-3T (=CCTCC AB 2010217T= KCTC 23196T=KACC 16433T).
authors.affiliation: Key Laboratory for Microbiological Engineering of Agricultural Environment of Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Zhi-Fei
authors.initials: ZF
authors.lastname: Lang
authors.affiliation: Key Laboratory for Microbiological Engineering of Agricultural Environment of Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Jin-Wei
authors.initials: JW
authors.lastname: Zheng
authors.affiliation: Key Laboratory for Microbiological Engineering of Agricultural Environment of Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Bao-Jian
authors.initials: BJ
authors.lastname: Hang
authors.affiliation: Key Laboratory for Microbiological Engineering of Agricultural Environment of Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Xiao-Qin
authors.initials: XQ
authors.lastname: Duan
authors.affiliation: Key Laboratory for Microbiological Engineering of Agricultural Environment of Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: He
authors.affiliation: Key Laboratory for Microbiological Engineering of Agricultural Environment of Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Shun-Peng
authors.initials: SP
authors.lastname: Li
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D001565:Benzoates; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*; D013095:Spermidine / Q000737:chemistry; D042301:Sphingomonadaceae / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D014451:Ubiquinone / Q000737:chemistry; D014865:Waste Disposal, Fluid
chemical_list: D001565:Benzoates; D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D014451:Ubiquinone; C017618:3-phenoxybenzoic acid; C025204:Ubiquinone Q2; D013095:Spermidine