title: A novel nanostructured iron oxide-gold bioelectrode for hydrogen peroxide sensing.
abstract: Fe(3)O(4) nanoparticles covalently linked to a gold electrode have been used for immobilizing catalase (CAT) enzyme to sense the presence of various concentrations of H(2)O(2). These nanoparticles ranging from 20 to 30 nm were synthesized by thermal co-precipitation of ferric and ferrous chlorides. SEM and XRD have been used for morphological and structural characterization of Fe(3)O(4) nanoparticles. CAT enzyme was linked covalently to the surface of iron oxide using carbodiimide in phosphate buffer (pH 7.4) at 4 °C. The enzyme-iron oxide link was confirmed by FT-IR spectroscopy. Sensing studies carried out using cyclic voltammetry showed a linear response of the CAT/nano Fe(3)O(4)/Au bioelectrode towards H(2)O(2) between 1.5 and 13.5 µM with a very sharp response time of 2 s.
authors.affiliation: Centre for Nanotechnology and Advanced Biomaterials, School of Chemical and Biotechnology, SASTRA University, Thanjavur, India.
authors.forename: Kavitha
authors.initials: K
authors.lastname: Thandavan
authors.forename: Sakthivel
authors.initials: S
authors.lastname: Gandhi
authors.forename: Swaminathan
authors.initials: S
authors.lastname: Sethuraman
authors.forename: John Bosco Balaguru
authors.initials: JB
authors.lastname: Rayappan
authors.forename: Uma Maheswari
authors.initials: UM
authors.lastname: Krishnan
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D002374:Catalase / Q000378:metabolism; D002417:Cattle; D055664:Electrochemical Techniques; D004566:Electrodes; D005290:Ferric Compounds / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000032:analysis*; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D005290:Ferric Compounds; C000499:ferric oxide; D006046:Gold; D006861:Hydrogen Peroxide; D002374:Catalase