title: Diagnostic significance of immunocytochemistry on fine needle aspiration biopsies processed by thin-layer cytology.
abstract: To evaluate the significance of immunocytochemistry (ICC) performed on Thin-Layer Cytology (TLC) preparations. During the period August 2004 to May 2010, 5,736 consecutive fine needle aspiration biopsies were processed by both conventional smears and TLC preparations, and 180 cases required further immunocytochemical staining to resolve diagnostic questions. TLC preparations were processed with the Thin-Layer Cytology 2000TM Method (Cytyc Corp, Marlborough, MA). Conventional smears and TLC preparations were stained with HE. ICC staining was carried out on TLC slides. All 180 cases were processed successfully for ICC. In 161 cases (89.5%), ICC staining resulted in a definitive diagnosis, while in 19 cases (10.5%) the diagnosis was still inconclusive. About 148 (82.2%) of the cases also had tissue available for histological examination. Accepting the histologic findings as the true diagnosis, the sensitivity and specificity of the ICC diagnoses were 98.6 and 97.3%, respectively. ICC can be successfully applied on TLC preparations, and its results can be very useful for making diagnoses on fine needle aspiration (FNA) biopsies.
authors.affiliation: Cytopathology Section, Cancer Hospital of the Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, People's Republic of China. zhangzhh9@163.com
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Zhang
authors.forename: Linlin
authors.initials: L
authors.lastname: Zhao
authors.forename: Huiqin
authors.initials: H
authors.lastname: Guo
authors.forename: Qinjing
authors.initials: Q
authors.lastname: Pan
authors.forename: Yuntian
authors.initials: Y
authors.lastname: Sun
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis* / Q000473:pathology; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D044963:Biopsy, Fine-Needle; D018278:Carcinoma, Neuroendocrine / Q000175:diagnosis* / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis* / Q000473:pathology; D003937:Diagnosis, Differential; D004701:Endocrine Gland Neoplasms / Q000175:diagnosis* / Q000473:pathology; D004801:Eosine Yellowish-(YS); D005260:Female; D006416:Hematoxylin; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D008228:Lymphoma, Non-Hodgkin / Q000175:diagnosis* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D004801:Eosine Yellowish-(YS); D006416:Hematoxylin