title: Protection against Escherichia coli O157:H7 challenge by immunization of mice with purified Tir proteins.
abstract: Enterohemorrhagic Escherichia coli (EHEC) O157:H7 infections cause serious public health problems worldwide. The translocation intimin receptor (Tir) is responsible for adhesion and attaching and effacing lesions. In the current study, we used a mitomycin-treated mouse model to evaluate the efficacy of subcutaneous vs intranasal administration of the recombinant Tir as vaccine. Following immunization, mice were infected with E. coli O157:H7 and faces were monitored for shedding. Mice immunized intrasally with purified Tir proteins produced higher IgG and IgA titers in serum and feces, resulting in significant reductions in fecal shedding of EHEC O157 and higher a survival rate (92.9%), compared with subcutaneous or control immunizations. These results demonstrate the potential for the use of Tir proteins in mucosal vaccine formulations to prevent colonization and shedding of E. coli O157:H7. Therefore, purified Tir protects mice against EHEC challenge after intranasal immunization and is worth further clinical development as a vaccine candidate.
authors.affiliation: School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, Guangdong Province, People's Republic of China.
authors.forename: Hong-Ying
authors.initials: HY
authors.lastname: Fan
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Luo
authors.forename: Bei-Guo
authors.initials: BG
authors.lastname: Long
mesh_terms: D000281:Administration, Intranasal / Q000379:methods*; D000818:Animals; D001428:Bacterial Vaccines / Q000008:administration & dosage*; D017931:DNA Primers / Q000235:genetics; D004927:Escherichia coli Infections / Q000517:prevention & control*; D019453:Escherichia coli O157 / Q000276:immunology*; D029968:Escherichia coli Proteins / Q000008:administration & dosage / Q000276:immunology*; D005243:Feces / Q000737:chemistry / Q000382:microbiology; D007070:Immunoglobulin A / Q000097:blood / Q000378:metabolism; D007074:Immunoglobulin G / Q000097:blood / Q000378:metabolism; D007279:Injections, Subcutaneous / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids / Q000235:genetics; D011956:Receptors, Cell Surface / Q000008:administration & dosage / Q000276:immunology*; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000276:immunology*; D013047:Specific Pathogen-Free Organisms; D016019:Survival Analysis
chemical_list: D001428:Bacterial Vaccines; D017931:DNA Primers; D029968:Escherichia coli Proteins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; C109287:Tir protein, E coli