title: [Classification of Pueraria lobata in different geographical regions].
abstract: OBJECTIVE
  To classify Pueraria lobata originated from different geographical regions based on ITS,psbK-psbI and trnH-psbA information.
  
  
  METHODS
  Twenty-four samples of P. lobata were collected from northeast China, north China, central China and northwest China. DNA extraction, PCR, sequence and genotypes/haplotypes analysis were performed .
  
  
  RESULTS
  ITS1, 5.8S and ITS2 varied only 1 bp respectively, psbK-psbI 2 bps; trnH-psbA varied 1 bp and 10 bp deletion.
  
  
  CONCLUSIONS
  Based on the variation of ITS,psbK-psbI and trnH-psbA, 4 genotypes and 2 haplotypes were identified, respectively.
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China. year_365@163.com
authors.forename: Dongji
authors.initials: D
authors.lastname: Liu
authors.forename: Zhikui
authors.initials: Z
authors.lastname: Yu
authors.forename: Chunsheng
authors.initials: C
authors.lastname: Liu
authors.forename: Xueyong
authors.initials: X
authors.lastname: Wang
mesh_terms: D001483:Base Sequence; D021903:DNA, Ribosomal Spacer; D050259:Genes, Mitochondrial; D005838:Genotype; D008969:Molecular Sequence Data; D009154:Mutation; D029908:Pueraria / Q000145:classification* / Q000235:genetics*
chemical_list: D021903:DNA, Ribosomal Spacer