title: G-quadruplex RNA structure as a signal for neurite mRNA targeting.
abstract: Targeting of messenger RNAs (mRNAs) in neuron processes relies on cis-acting regulatory elements, the nature of which is poorly understood. Here, we report that approximately 30% of the best-known dendritic mRNAs contain a guanine (G)-quadruplex consensus in their 3'-untranslated region. Among these mRNAs, we show by using RNA structure probing that a G-quadruplex is present in the mRNAs of two key postsynaptic proteins: PSD-95 and CaMKIIa. The G-quadruplex structure is necessary and sufficient for the potent and fast localization of mRNAs in cortical neurites and this occurs in a metabotropic glutamate receptor-responsive manner. Thus, G-quadruplex seems to be a common neurite localization signal.
authors.affiliation: IGBMC (Institut de Génétique et de Biologie Moléculaire et Cellulaire), CNRS, UMR7104, Inserm, U596, Collège de France, Strasbourg University, Illkirch-Graffenstaden 67404, France.
authors.forename: Murugan
authors.initials: M
authors.lastname: Subramanian
authors.forename: Florence
authors.initials: F
authors.lastname: Rage
authors.forename: Ricardos
authors.initials: R
authors.lastname: Tabet
authors.forename: Eric
authors.initials: E
authors.lastname: Flatter
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Mandel
authors.forename: Hervé
authors.initials: H
authors.lastname: Moine
mesh_terms: D000818:Animals; D001483:Base Sequence; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2 / Q000235:genetics; D000076122:Disks Large Homolog 4 Protein; D054856:G-Quadruplexes; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D016501:Neurites / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D034443:RNA Transport; D012333:RNA, Messenger / Q000737:chemistry* / Q000378:metabolism*
chemical_list: C501444:DLG4 protein, human; D000076122:Disks Large Homolog 4 Protein; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D012333:RNA, Messenger; C517657:CAMK2A protein, human; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2