title: Biomarkers for severity of spinal cord injury in the cerebrospinal fluid of rats.
abstract: One of the major challenges in management of spinal cord injury (SCI) is that the assessment of injury severity is often imprecise. Identification of reliable, easily quantifiable biomarkers that delineate the severity of the initial injury and that have prognostic value for the degree of functional recovery would significantly aid the clinician in the choice of potential treatments. To find such biomarkers we performed quantitative liquid chromatography-mass spectrometry (LC-MS/MS) analyses of cerebrospinal fluid (CSF) collected from rats 24 h after either a moderate or severe SCI. We identified a panel of 42 putative biomarkers of SCI, 10 of which represent potential biomarkers of SCI severity. Three of the candidate biomarkers, Ywhaz, Itih4, and Gpx3 were also validated by Western blot in a biological replicate of the injury. The putative biomarkers identified in this study may potentially be a valuable tool in the assessment of the extent of spinal cord damage.
authors.affiliation: Department of Zoology, Life Sciences Institute, University of British Columbia, Vancouver, British Columbia, Canada. jlubieni@interchange.ubc.ca
authors.forename: Joanna M
authors.initials: JM
authors.lastname: Lubieniecka
authors.forename: Femke
authors.initials: F
authors.lastname: Streijger
authors.forename: Jae H T
authors.initials: JH
authors.lastname: Lee
authors.forename: Nikolay
authors.initials: N
authors.lastname: Stoynov
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.forename: Randy
authors.initials: R
authors.lastname: Mottus
authors.forename: Tom
authors.initials: T
authors.lastname: Pfeifer
authors.forename: Brian K
authors.initials: BK
authors.lastname: Kwon
authors.forename: Jens R
authors.initials: JR
authors.lastname: Coorssen
authors.forename: Leonard J
authors.initials: LJ
authors.lastname: Foster
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Grigliatti
authors.forename: Wolfram
authors.initials: W
authors.lastname: Tetzlaff
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000134:cerebrospinal fluid* / Q000378:metabolism; D015153:Blotting, Western; D002853:Chromatography, Liquid / Q000379:methods; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D010455:Peptides / Q000737:chemistry; D011379:Prognosis; D040901:Proteomics / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000473:pathology; D013119:Spinal Cord Injuries / Q000134:cerebrospinal fluid* / Q000175:diagnosis*; D013997:Time Factors
chemical_list: D015415:Biomarkers; D010455:Peptides