title: A novel synthesis of iminodiacetic acid: biocatalysis by whole Alcaligenes faecalis ZJB-09133 cells from iminodiacetonitrile.
abstract: Iminodiacetic acid (IDA) has been widely used as an important intermediate in the fine chemical industry. In this study, a novel synthesis route of IDA from iminodiacetonitrile by whole microorganisms was investigated. A strain with the capability of producing nitrilase, ZJB-09133, was isolated and identified, and later named Alcaligenes faecalis ZJB-09133. In addition, the detailed biocatalysis of iminodiacetonitrile to produce IDA using ZJB-09133 was investigated. The results showed that the conversion reached 65.3% in Na(2)HPO(4)-NaH(2)PO(4) buffer of pH 8.0 under the following conditions: cells in the amount of 0.075-g DCW/L, 1.5% substrate, conversion time of 8 h, and a reaction temperature of 35°C. To the best of our knowledge, this is the first time that the production of IDA using a biocatalysis method has been reported.
authors.affiliation: Institute of Bioengineering, Zhejiang University of Technology, Hangzhou 310014, People's Republic of China.
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Liu
authors.forename: Fei-Fei
authors.initials: FF
authors.lastname: Li
authors.forename: Feng
authors.initials: F
authors.lastname: Cheng
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.forename: Zhong-Yu
authors.initials: ZY
authors.lastname: You
authors.forename: Jian-Miao
authors.initials: JM
authors.lastname: Xu
authors.forename: Ya-Ping
authors.initials: YP
authors.lastname: Xue
authors.forename: Yu-Guo
authors.initials: YG
authors.lastname: Zheng
authors.forename: Yin-Chu
authors.initials: YC
authors.lastname: Shen
mesh_terms: D000097:Acetonitriles / Q000378:metabolism*; D042481:Alcaligenes faecalis / Q000201:enzymology / Q000302:isolation & purification / Q000378:metabolism*; D000619:Aminohydrolases / Q000378:metabolism; D055162:Biocatalysis; D002021:Buffers; D007098:Imino Acids / Q000138:chemical synthesis*; D007700:Kinetics
chemical_list: D000097:Acetonitriles; D002021:Buffers; D007098:Imino Acids; D000619:Aminohydrolases; C021867:nitrilase; C008109:iminodiacetic acid