title: PERMANENT GENETIC RESOURCES: Identification of nuclear microsatellite loci for Ipomopsis aggregata and the distribution of pairwise relatedness in a natural population.
abstract: Nine microsatellite loci were developed from enriched libraries of scarlet gilia (Ipomopsis aggregata). A screen of 160 individuals from a population identified reduced levels of heterozygosity, low levels of relatedness, and weak spatial genetic patterns. The population inbreeding coefficient was calculated to be 0.19 (SE = 0.04). These patterns are consistent with those expected from low levels of biparental inbreeding in an obligate outcrosser and extensive seed and pollen dispersal. These preliminary data confirm the usefulness of microsatellite markers for breeding system studies of I. aggregata.
authors.affiliation: Section of Integrative Biology, The University of Texas at Austin, 1 University Station, C0930, Austin, Texas 78712, USA, Department of Biology, Duke University, Box 90338, Durham, North Carolina 27708, USA.
authors.forename: Frank
authors.initials: F
authors.lastname: Stearns
authors.forename: Sandra
authors.initials: S
authors.lastname: Boles
authors.forename: Heather
authors.initials: H
authors.lastname: Hurston
authors.forename: Tanya
authors.initials: T
authors.lastname: Vo
authors.forename: Dale
authors.initials: D
authors.lastname: Butler
authors.forename: Walter
authors.initials: W
authors.lastname: Shuham
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Juenger