title: Aberrant phosphorylation of STAT5 by granulocyte-macrophage colony-stimulating factor in infant cytomegalovirus infection mimicking juvenile myelomonocytic leukemia.
abstract: Juvenile myelomonocytic leukemia (JMML) progenitor cells exhibit in vitro hypersensitivity to granulocyte-macrophage colony-stimulating factor (GM-CSF). Phospho-specific flow cytometry using anti-phosphorylated STAT5 antibody is a new method recently reported to detect GM-CSF hypersensitivity of cells. However, colony assays using methylcellulose medium to measure GM-CSF-hypersensitivity remain as the current gold standard. Interestingly, cytomegalovirus (CMV) infection in infancy often presents with a variety of clinical symptoms that mimic JMML, with CMV giving a positive result by colony assay. We wanted to determine whether aberrant STAT5 activation occurs in CMV infection by using phospho-specific flow cytometry, and to ascertain whether this method is effective at discriminating CMV infection from JMML. Peripheral blood mononuclear cells from patients with JMML and CMV infection displayed an elevated proportion of p-STAT5 cells after low-dose GM-CSF stimulation when compared with cells from normal individuals. However, we found no significant differences in the percentage of p-STAT5 positive cells from patients with CMV infection and JMML at any doses of the GM-CSF doses used. We conclude that patients with CMV infection cannot be discriminated from patients with JMML by this new diagnostic method.
authors.affiliation: Department of Pediatrics, Nagoya University Graduate School of Medicine, Shouwa-ku, Nagoya, Japan.
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Nishio
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Makito
authors.initials: M
authors.lastname: Tanaka
authors.forename: Yinyan
authors.initials: Y
authors.lastname: Xu
authors.forename: Nao
authors.initials: N
authors.lastname: Yoshida
authors.forename: Hirotoshi
authors.initials: H
authors.lastname: Sakaguchi
authors.forename: Sayoko
authors.initials: S
authors.lastname: Doisaki
authors.forename: Asahito
authors.initials: A
authors.lastname: Hama
authors.forename: Hideki
authors.initials: H
authors.lastname: Muramatsu
authors.forename: Akira
authors.initials: A
authors.lastname: Shimada
authors.forename: Seiji
authors.initials: S
authors.lastname: Kojima
mesh_terms: D002675:Child, Preschool; D003586:Cytomegalovirus Infections / Q000097:blood / Q000151:congenital / Q000175:diagnosis* / Q000378:metabolism*; D003937:Diagnosis, Differential; D004526:Efficiency; D005260:Female; D005434:Flow Cytometry; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000097:blood / Q000378:metabolism* / Q000502:physiology; D006801:Humans; D007223:Infant; D054429:Leukemia, Myelomonocytic, Juvenile / Q000097:blood / Q000175:diagnosis* / Q000378:metabolism; D008297:Male; D010766:Phosphorylation; D050799:STAT5 Transcription Factor / Q000097:blood / Q000378:metabolism*; D012680:Sensitivity and Specificity
chemical_list: D050799:STAT5 Transcription Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor