title: Isolation and characterization of microsatellite markers for the ornamental discus fish Symphysodon discus and cross-species amplification in other Heroini cichlid species.
abstract: The discus fishes (Symphysodon spp.) are economically important ornamental species. Thirteen microsatellite markers were developed from a CT(12) - and CA(12) -enriched whole genomic DNA library of Symphysodon discus. Allelic variability was tested on 44 individuals of two species (S. discus and S. aequifasciatus). Allelic richness ranged from two to 11 alleles per locus and observed heterozygosities from 0.083 to 0.998. All loci were at Hardy-Weinberg equilibrium, and no pair of loci showed linkage disequilibrium within a species. Cross-species amplification was also successfully performed in the Neotropical cichlids Uaru amphiacanthoides, Hoplarchus psittacus, Hypselecara coryphaenoides, Pterophyllum sp., Mesonauta sp. and Heros sp.
authors.affiliation: Departamento de Biologia, Laboratório de Evolução e Genética Animal, Universidade Federal do Amazonas, Av. Rodrigo Octávio Jordão Ramos, 3000, 69077-000, Manaus, Amazonas, Brazil, Biology Department, University of Puerto Rico - Rio Piedras, San Juan, PR 00931, Puerto Rico, Centro de Apoio Muldisciplinar, Laboratório de Tecnologias de DNA, Universidade Federal do Amazonas, Av. Rodrigo Octávio Jordão Ramos, 3000, 69077-000, Manaus, Amazonas, Brazil.
authors.forename: Manuella Villar
authors.initials: MV
authors.lastname: Amado
authors.forename: Tomas
authors.initials: T
authors.lastname: Hrbek
authors.forename: Waleska
authors.initials: W
authors.lastname: Gravena
authors.forename: Cleiton
authors.initials: C
authors.lastname: Fantin
authors.forename: Enedina Nogueira
authors.initials: EN
authors.lastname: DE Assunção
authors.forename: Spartaco
authors.initials: S
authors.lastname: Astolfi-Filho
authors.forename: Izeni P
authors.initials: IP
authors.lastname: Farias