title: Immunohistochemical Evaluation of p63, E-Cadherin, Collagen I and III Expression in Lower Limb Wound Healing under Honey.
abstract: Honey is recognized traditionally for its medicinal properties and also appreciated as a topical healing agent for infected and noninfected wounds. This study evaluates impact of honey-based occlusive dressing on nonhealing (nonresponding to conventional antibiotics) traumatic lower limb wounds (n = 34) through clinicopathological and immunohistochemical (e.g., expression of p63, E-cadherin, and Collagen I and III) evaluations to enrich the scientific validation. Clinical findings noted the nonadherence of honey dressing with remarkable chemical debridement and healing progression within 11-15 days of postintervention. Histopathologically, in comparison to preintervention biopsies, the postintervention tissues of wound peripheries demonstrated gradual normalization of epithelial and connective tissue features with significant changes in p63(+) epithelial cell population, reappearance of membranous E-cadherin (P < .0001), and optimum deposition of collagen I and III (P < .0001). Thus, the present study for the first time reports the impact of honey on vital protein expressions in epithelial and connective tissues during repair of nonhealing lower limb wounds.
authors.affiliation: School of Medical Science & Technology, Indian Institute of Technology, Kharagpur 721302, India.
authors.forename: Ananya
authors.initials: A
authors.lastname: Barui
authors.forename: Provas
authors.initials: P
authors.lastname: Banerjee
authors.forename: Raunak Kumar
authors.initials: RK
authors.lastname: Das
authors.forename: Shyamal Kumar
authors.initials: SK
authors.lastname: Basu
authors.forename: Santanu
authors.initials: S
authors.lastname: Dhara
authors.forename: Jyotirmoy
authors.initials: J
authors.lastname: Chatterjee