title: [The effect of high frequency stimulation of epileptic foci on the release of glutamate and gamma-aminobutyric acid in hippocampus of the kainic acid-kindled rats].
abstract: OBJECTIVE
  To observe the dynamics of hippocampal release of glutamate (Glu) and gamma-aminobutyric acid (GABA) in epilepsy (TLE) after administration with high frequency stimulation (HFS).
  
  
  METHODS
  The SD were divided into four groups (n =10): (1) Control group (KB) the rats were injected intraperitoneally with saline 0.9%. (2) Kainic acid (KA) group: the rats were injected with KA. (3) Pseudo-deep brain stimulation (DBS) group: the KA-induced rats were implanted with rheophores alone. (4) DBS group: KA induced-rats with DBS in hippocampal epileptic foci. We then collected hippocampal extracellular fluid by microdialysis and the levels of Glu and GABA were measured by high-performance liquid chromatography (HPLC) and fluorescence detection.
  
  
  RESULTS
  There was no difference in the baseline of Glu and GABA in the four groups. In contrast, a significant increase in the content of Glu and GABA was shown in the three periods of KA-kindled seizures. Electrical stimulation of hippocampus resulted in a decrease of hippocampal Glu contents, while there was no change in GABA contents. Additionally, HFS of hippocampus normalized the Glu/GABA ratio in the chronic period of seizures.
  
  
  CONCLUSIONS
  The high frequency stimulation of epileptic foci may protect against seizures by modulating the extracellular release of hippocampal Glu.
authors.affiliation: Department of Neurology, Union Hospital, Fujian Medical University, Fuzhou 350001, China.
authors.forename: Wan-Hui
authors.initials: WH
authors.lastname: Lin
authors.forename: Hua-Pin
authors.initials: HP
authors.lastname: Huang
authors.forename: Ming-Xing
authors.initials: MX
authors.lastname: Lin
authors.forename: Sheng-Gen
authors.initials: SG
authors.lastname: Chen
mesh_terms: D000818:Animals; D004558:Electric Stimulation / Q000379:methods*; D004827:Epilepsy / Q000139:chemically induced / Q000628:therapy*; D018698:Glutamic Acid / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism*; D007608:Kainic Acid; D007696:Kindling, Neurologic / Q000187:drug effects; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
chemical_list: D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid; D007608:Kainic Acid