title: Xenotropic murine leukemia virus-related virus-associated chronic fatigue syndrome reveals a distinct inflammatory signature.
abstract: BACKGROUND
  The recent identification of xenotropic murine leukemia virus-related virus (XMRV) in the blood of patients with chronic fatigue syndrome (CFS) establishes that a retrovirus may play a role in the pathology in this disease. Knowledge of the immune response might lead to a better understanding of the role XMRV plays in this syndrome. Our objective was to investigate the cytokine and chemokine response in XMRV-associated CFS.
  
  
  METHODS
  Using Luminex multi-analyte profiling technology, we measured cytokine and chemokine values in the plasma of XMRV-infected CFS patients and compared these data to those of healthy controls. Analysis was performed using the Gene Expression Pattern Analysis Suite and the Random Forest tree classification algorithm.
  
  
  RESULTS
  This study identifies a signature of 10 cytokines and chemokines which correctly identifies XMRV/CFS patients with 93% specificity and 96% sensitivity.
  
  
  CONCLUSIONS
  These data show, for the first time, an immunological pattern associated with XMRV/CFS.
authors.affiliation: Whittemore Peterson Institute, University of Nevada, Reno MS 0552, 1664 N. Virginia St. Reno, NV 89557-0552, USA. vclombardi@wpinstitute.org
authors.forename: Vincent C
authors.initials: VC
authors.lastname: Lombardi
authors.forename: Kathryn S
authors.initials: KS
authors.lastname: Hagen
authors.forename: Kenneth W
authors.initials: KW
authors.lastname: Hunter
authors.forename: John W
authors.initials: JW
authors.lastname: Diamond
authors.forename: Julie
authors.initials: J
authors.lastname: Smith-Gagen
authors.forename: Wei
authors.initials: W
authors.lastname: Yang
authors.forename: Judy A
authors.initials: JA
authors.lastname: Mikovits
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016000:Cluster Analysis; D016207:Cytokines / Q000097:blood; D015673:Fatigue Syndrome, Chronic / Q000097:blood / Q000503:physiopathology* / Q000821:virology; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007249:Inflammation / Q000097:blood; D008297:Male; D008875:Middle Aged; D008954:Models, Biological; D012680:Sensitivity and Specificity; D013601:T-Lymphocytes / Q000276:immunology; D058008:Xenotropic murine leukemia virus-related virus / Q000502:physiology*; D055815:Young Adult
chemical_list: D016207:Cytokines