title: Isolation of nine nuclear microsatellites in the common Mediterranean sea urchin, Paracentrotus lividus (Lamarck).
abstract: Nine polymorphic microsatellite loci were isolated and characterized for the edible common sea urchin Paracentrotus lividus. Loci were obtained from two genomic libraries enriched with different di-, tri- and tetranucleotides. Most microsatellites obtained were imperfect dinucleotides. Allelic variation was screened for a total of 56 individuals from two populations from North-Western Mediterranean. Microsatellites showed a number of alleles ranging from 13 to 35. Linkage disequilibrium was not detected between any pair of loci, and all loci showed a significant departure from Hardy-Weinberg equilibrium which is not likely to be the result of null alleles, suggesting that demographic features may be acting upon this commercially interesting species.
authors.affiliation: Department of Animal Biology (Invertebrates), Faculty of Biology, University of Barcelona, 645 Diagonal Avenue, 08028 Barcelona, Spain. calderon@ub.edu
authors.forename: I
authors.initials: I
authors.lastname: Calderón
authors.forename: X
authors.initials: X
authors.lastname: Turon
authors.forename: M
authors.initials: M
authors.lastname: Pascual