title: Flavobacterium yonginense sp. nov. and Flavobacterium myungsuense sp. nov., isolated from a mesotrophic artificial lake.
abstract: Two non-motile, Gram-staining-negative, yellow-pigmented bacterial strains designated HMD1001T and HMD1033T were isolated from the water of a mesotrophic artificial lake in Korea. A phylogenetic tree based on 16S rRNA gene sequences indicated that both strains could be assigned to the genus Flavobacterium; strain HMD1001T appeared most closely related to Flavobacterium fluvii H7T (96.8 % sequence similarity), F. succinicans DSM 4002T (96.6 %) and F. hydatis DSM 2063T (96.6 %) whereas strain HMD1033T appeared most closely related to Flavobacterium psychrolimnae LMG 2201T (96.2 %), F. segetis AT1048T (96.2 %) and F. weaverense AT1042T (96.2 %). The major fatty acids of strain HMD1001T were iso-C15:0 (21.5 %), summed feature 3 (comprising C16:1ω6c and/or C16:1ω7c; 18.0 %) and iso-C15:1 G (7.6 %), whereas those of HMD1033T were summed feature 3 (23.8 %), iso-C15:0 3-OH (16.9 %), iso-C15:0 (15.3 %) and anteiso-C15:0 (12.1 %). The genomic DNA G+C contents of strains HMD1001T and HMD1033T were 35.9 and 32.2 mol%, respectively. Phylogenetic and phenotypic evidence indicates that strains HMD1001T and HMD1033T represent two novel species of the genus Flavobacterium, for which the names Flavobacterium yonginense sp. nov. (type strain HMD1001T=KCTC 22796T=CECT 7594T) and Flavobacterium myungsuense sp. nov. (type strain HMD1033T=KCTC 22825T=CECT 7649T) are proposed.
authors.affiliation: Department of Environmental Science, Kangwon National University, Chuncheon 200-701, Republic of Korea.|Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies, Gyeonggi 449-791, Republic of Korea.
authors.forename: Yochan
authors.initials: Y
authors.lastname: Joung
authors.affiliation: Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies, Gyeonggi 449-791, Republic of Korea.
authors.forename: Haneul
authors.initials: H
authors.lastname: Kim
authors.affiliation: Department of Environmental Science, Kangwon National University, Chuncheon 200-701, Republic of Korea.
authors.forename: Tae-Seok
authors.initials: TS
authors.lastname: Ahn
authors.affiliation: Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies, Gyeonggi 449-791, Republic of Korea.
authors.forename: Kiseong
authors.initials: K
authors.lastname: Joh
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005417:Flavobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D060106:Lakes / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010858:Pigmentation; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S