title: iCLIP--transcriptome-wide mapping of protein-RNA interactions with individual nucleotide resolution.
abstract: The unique composition and spatial arrangement of RNA-binding proteins (RBPs) on a transcript guide the diverse aspects of post-transcriptional regulation. Therefore, an essential step towards understanding transcript regulation at the molecular level is to gain positional information on the binding sites of RBPs. Protein-RNA interactions can be studied using biochemical methods, but these approaches do not address RNA binding in its native cellular context. Initial attempts to study protein-RNA complexes in their cellular environment employed affinity purification or immunoprecipitation combined with differential display or microarray analysis (RIP-CHIP). These approaches were prone to identifying indirect or non-physiological interactions. In order to increase the specificity and positional resolution, a strategy referred to as CLIP (UV cross-linking and immunoprecipitation) was introduced. CLIP combines UV cross-linking of proteins and RNA molecules with rigorous purification schemes including denaturing polyacrylamide gel electrophoresis. In combination with high-throughput sequencing technologies, CLIP has proven as a powerful tool to study protein-RNA interactions on a genome-wide scale (referred to as HITS-CLIP or CLIP-seq). Recently, PAR-CLIP was introduced that uses photoreactive ribonucleoside analogs for cross-linking. Despite the high specificity of the obtained data, CLIP experiments often generate cDNA libraries of limited sequence complexity. This is partly due to the restricted amount of co-purified RNA and the two inefficient RNA ligation reactions required for library preparation. In addition, primer extension assays indicated that many cDNAs truncate prematurely at the crosslinked nucleotide. Such truncated cDNAs are lost during the standard CLIP library preparation protocol. We recently developed iCLIP (individual-nucleotide resolution CLIP), which captures the truncated cDNAs by replacing one of the inefficient intermolecular RNA ligation steps with a more efficient intramolecular cDNA circularization (Figure 1). Importantly, sequencing the truncated cDNAs provides insights into the position of the cross-link site at nucleotide resolution. We successfully applied iCLIP to study hnRNP C particle organization on a genome-wide scale and assess its role in splicing regulation.
authors.affiliation: Laboratory of Molecular Biology, Medical Research Council - MRC.
authors.forename: Julian
authors.initials: J
authors.lastname: Konig
authors.forename: Kathi
authors.initials: K
authors.lastname: Zarnack
authors.forename: Gregor
authors.initials: G
authors.lastname: Rot
authors.forename: Tomaz
authors.initials: T
authors.lastname: Curk
authors.forename: Melis
authors.initials: M
authors.lastname: Kayikci
authors.forename: Blaz
authors.initials: B
authors.lastname: Zupan
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Turner
authors.forename: Nicholas M
authors.initials: NM
authors.lastname: Luscombe
authors.forename: Jernej
authors.initials: J
authors.lastname: Ule
mesh_terms: D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods*; D047468:Immunoprecipitation / Q000379:methods; D012313:RNA / Q000032:analysis* / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects; D012326:RNA Splicing; D016601:RNA-Binding Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D018076:DNA, Complementary; D016601:RNA-Binding Proteins; D012313:RNA