title: Comparative in vivo antioxidant capacity of DL-2-hydroxy-4-methylthiobutanoic acid (HMTBA) and DL-methionine in male mice fed a high-fat diet.
abstract: BACKGROUND
  In animal diets, methionine (Met) is considered to be the first limiting amino acid, and the activity of synthetic Met is typically added either as DL-methionine (DLM) or as DL-2-hydroxy-4-methylthiobutanoic acid (HMTBA). It has been demonstrated that HMTBA exhibits a higher antioxidant capability in vitro as compared to DLM. However, the difference in antioxidant capability between DLM and HMTBA in vivo is unknown.
  
  
  METHODS
  In the present study, 60 male C57BL/6 mice were randomly divided into six groups and fed either a normal diet (NFD, 5.37% fat) or a high-fat diet (HFD, 19.7% fat) in conjunction with 0.2% DLM, 0.2% HMTBA or 0.1% DLM and 0.1% HMTBA for 4 weeks.
  
  
  RESULTS
  HFD supplemented with 2% DLM and NFD with 2% HMTBA both induced adverse affects in relation to serum lipid parameters and depressed antioxidant defense systems in the digestive system. However, these changes were restored in the 0.2% HMTBA-treated HFD group. Furthermore, no significant differences were found in the lipid parameters and antioxidant status in the NFD and HFD group supplemented with 0.1% DLM and 0.1% HMTBA.
  
  
  CONCLUSIONS
  HMTBA restored oxidative redox status under OS conditions and its antioxidant properties were positively correlated with the dosage included in diet.
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, Jiangsu Province, PR China.
authors.forename: Xue
authors.initials: X
authors.lastname: Tang
authors.forename: Yonglan
authors.initials: Y
authors.lastname: Yang
authors.forename: Yonghui
authors.initials: Y
authors.lastname: Shi
authors.forename: Guowei
authors.initials: G
authors.lastname: Le
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000627:therapeutic use*; D050197:Atherosclerosis / Q000517:prevention & control; D004041:Dietary Fats / Q000009:adverse effects; D006949:Hyperlipidemias / Q000097:blood / Q000378:metabolism / Q000517:prevention & control*; D000960:Hypolipidemic Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D015227:Lipid Peroxidation; D008055:Lipids / Q000097:blood; D008297:Male; D008715:Methionine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000627:therapeutic use*; D051379:Mice; D008810:Mice, Inbred C57BL; D009753:Nutritive Value; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010088:Oxidoreductases / Q000097:blood / Q000378:metabolism*; D011897:Random Allocation; D017382:Reactive Oxygen Species / Q000097:blood / Q000378:metabolism
chemical_list: D000975:Antioxidants; D004041:Dietary Fats; D000960:Hypolipidemic Agents; D008055:Lipids; D017382:Reactive Oxygen Species; C008391:alpha-hydroxy-gamma-methylmercaptobutyric acid; D008715:Methionine; D010088:Oxidoreductases