title: In vivo fluorescence lifetime optical projection tomography.
abstract: We demonstrate the application of fluorescence lifetime optical projection tomography (FLIM-OPT) to in vivo imaging of lysC:GFP transgenic zebrafish embryos (Danio rerio). This method has been applied to unambiguously distinguish between the fluorescent protein (GFP) signal in myeloid cells from background autofluorescence based on the fluorescence lifetime. The combination of FLIM, an inherently ratiometric method, in conjunction with OPT results in a quantitative 3-D tomographic technique that could be used as a robust method for in vivo biological and pharmaceutical research, for example as a readout of Förster resonance energy transfer based interactions.
authors.forename: James
authors.initials: J
authors.lastname: McGinty
authors.forename: Harriet B
authors.initials: HB
authors.lastname: Taylor
authors.forename: Lingling
authors.initials: L
authors.lastname: Chen
authors.forename: Laurence
authors.initials: L
authors.lastname: Bugeon
authors.forename: Jonathan R
authors.initials: JR
authors.lastname: Lamb
authors.forename: Margaret J
authors.initials: MJ
authors.lastname: Dallman
authors.forename: Paul M W
authors.initials: PM
authors.lastname: French