title: Microsatellite markers for mungbean developed from sequence database.
abstract: A novel set of microsatellite markers for mungbean [Vigna radiata (L.) Wilczek] was developed from the public sequence database. Seventy-eight primers were designed and evaluated for polymorphism among 22 cultivated accessions. Eight polymorphic loci detected two to three alleles per locus with an average of 2.25. The observed heterozygosity varied from 0.00 to 0.18, while the expected heterozygosity ranged from 0.09 to 0.46. Among them, all eight loci showed significant departuring from Hardy-Weinberg equilibrium, while four pairs of loci displayed significant pairwise linkage disequilibrium values. All eight loci except DMB-SSR1 showed heterozygote deficiency.
authors.affiliation: Department of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Nakhon Pathom 73140, Thailand.
authors.forename: Worapa
authors.initials: W
authors.lastname: Seehalak
authors.forename: Prakit
authors.initials: P
authors.lastname: Somta
authors.forename: Warunee
authors.initials: W
authors.lastname: Sommanas
authors.forename: Peerasak
authors.initials: P
authors.lastname: Srinives