title: Involvement of antioxidant enzymes in multiple-drug resistance in a human T-lymphoblastic leukemia-cell line which over-expresses p-glycoprotein.
abstract: We have compared the specific activities of Cu/Zn superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) in a vinblastine sensitive human T-lymphoblastic cell line (CCRF-CEM) and its multiple drug resistant (MDR) counterpart cell line (CEM/VLB100), which over-expresses P-glycoprotein (PGP). We have found that the specific activity Cu/Zn SOD was consistently 38% increased in CEM/VLB100 cells compared with CCRF-CEM cells. In contrast, the activities of CAT and GSH-Px were similar in the two cell lines. These results suggest that MDR in CEM/VLB100 is a complicated phenotype which not only involves a PGP mechanism, but also a SOD protection mechanism against drug-mediated O2.- cytotoxicity.
authors.affiliation: UNIV LONDON LONDON HOSP,COLL MED,INFLAMMAT RES GRP,ARTHRIT & RHEUMATISM COUNCIL BLDG,LONDON E1 2AD,ENGLAND. UNIV LONDON LONDON HOSP,COLL MED,DEPT HAEMATOL,LONDON E1 2AD,ENGLAND.
authors.forename: M
authors.initials: M
authors.lastname: Yang
authors.forename: X
authors.initials: X
authors.lastname: Jiang
authors.forename: D
authors.initials: D
authors.lastname: Blake
authors.forename: Z
authors.initials: Z
authors.lastname: Zhang
authors.forename: M
authors.initials: M
authors.lastname: Macey
authors.forename: A
authors.initials: A
authors.lastname: Newland
authors.forename: C
authors.initials: C
authors.lastname: Morris