title: Detection of antibiotic residues in bovine milk by a voltammetric electronic tongue system.
abstract: A voltammetric electronic tongue (VE-tongue) was developed to detect antibiotic residues in bovine milk. Six antibiotics (Chloramphenicol, Erythromycin, Kanamycin sulfate, Neomycin sulfate, Streptomycin sulfate and Tetracycline HCl) spiked at four different concentration levels (0.5, 1, 1.5 and 2 maximum residue limits (MRLs)) were classified based on VE-tongue by two pattern recognition methods: principal component analysis (PCA) and discriminant function analysis (DFA). The VE-tongue was composed of five working electrodes (gold, silver, platinum, palladium, and titanium) positioned in a standard three-electrode configuration. The Multi-frequency large amplitude pulse voltammetry (MLAPV) which consisted of four segments (1 Hz, 10 Hz, 100 Hz and 1000 Hz) was applied as potential waveform. The six antibiotics at the MRLs could not be separated from bovine milk completely by PCA, but all the samples were demarcated clearly by DFA. Three regression models: Principal Component Regression Analysis (PCR), Partial Least Squares Regression (PLSR), and Least Squares-Support Vector Machines (LS-SVM) were used for concentrations of antibiotics prediction. All the regression models performed well, and PCR had the most stable results.
authors.affiliation: Zhejiang University, Department of Bio-Systems Engineering, Hangzhou, Zhejiang, China.
authors.forename: Zhenbo
authors.initials: Z
authors.lastname: Wei
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002417:Cattle; D002701:Chloramphenicol / Q000032:analysis; D016002:Discriminant Analysis; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D004917:Erythromycin / Q000032:analysis; D007612:Kanamycin / Q000032:analysis; D016018:Least-Squares Analysis; D008892:Milk / Q000737:chemistry*; D009355:Neomycin / Q000032:analysis; D025341:Principal Component Analysis; D013307:Streptomycin / Q000032:analysis; D013752:Tetracycline / Q000032:analysis
chemical_list: D000900:Anti-Bacterial Agents; D007612:Kanamycin; D004917:Erythromycin; D002701:Chloramphenicol; D013752:Tetracycline; D009355:Neomycin; D013307:Streptomycin