title: Genetic diversity of HIV type 1 isolated from newly diagnosed subjects (2006-2007) in Beijing, China.
abstract: The purpose of this study was to investigate the genetic diversity of HIV-1 circulating in Beijing and its molecular epidemiological linkages with regard to risk factors of viral transmission. HIV-1 from plasma samples of 280 diagnosed individuals (2006-2007) was characterized. The gene fragments of gag, pol, and env from the infected plasma samples were amplified by reverse transcriptase polymerase chain reaction (RT-PCR), sequenced, and phylogenetically analyzed. From the 280 plasma samples analyzed, a total of 496 sequences were successfully amplified from the gag, pol, and env genes. Nine HIV-1 group M subtypes or CRF including A1, B, B', C, CRF01_AE, CRF02_AG, CRF06_cpx, CRF07_BC, and CRF08_BC, and six new B'/C recombinants were identified. CRF07_BC was found to be the most dominant subtype (32.5%) followed by CRF01_AE (25.0%), B (20.0%), and B' (15.7%). The data from this study indicate the existence of multiple HIV-1 subtypes or CRFs in Beijing and may be proven useful in the development of vaccine candidates in the future.
authors.affiliation: National Institute for Viral Disease Control and Prevention, China Center for Disease Prevention and Control, Beijing, China.
authors.forename: Jing-rong
authors.initials: JR
authors.lastname: Ye
authors.forename: Shuang-qing
authors.initials: SQ
authors.lastname: Yu
authors.forename: Hong-yan
authors.initials: HY
authors.lastname: Lu
authors.forename: Wei-shi
authors.initials: WS
authors.lastname: Wang
authors.forename: Ruo-lei
authors.initials: RL
authors.lastname: Xin
authors.forename: Yi
authors.initials: Y
authors.lastname: Zeng
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D044466:Asian People / Q000706:statistics & numerical data; D002681:China / Q000453:epidemiology; D016000:Cluster Analysis; D005260:Female; D014644:Genetic Variation; D006679:HIV Seropositivity / Q000453:epidemiology / Q000235:genetics*; D015497:HIV-1 / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008875:Middle Aged; D017720:Molecular Epidemiology; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D011795:Surveys and Questionnaires; D055815:Young Adult; D054299:env Gene Products, Human Immunodeficiency Virus / Q000235:genetics*; D054301:gag Gene Products, Human Immunodeficiency Virus / Q000235:genetics*; D054302:pol Gene Products, Human Immunodeficiency Virus / Q000235:genetics*
chemical_list: D054299:env Gene Products, Human Immunodeficiency Virus; D054301:gag Gene Products, Human Immunodeficiency Virus; D054302:pol Gene Products, Human Immunodeficiency Virus