title: CD209/DC-SIGN mediates efficient infection of monocyte-derived dendritic cells by clinical adenovirus 2C isolates in the presence of bovine lactoferrin.
abstract: Adenovirus often causes respiratory infection in immunocompromised patients, but relevant attachment receptors have largely not been defined. We show that the antiviral protein bovine lactoferrin enhances infection of monocyte-derived dendritic cells (MDDC) by adenovirus species C serotype 2 (2C) isolates. Under the same conditions infection of MDDC by human( )cytomegalovirus was reduced. Adenoviral infection was prominently enhanced by bovine but not human lactoferrin, and was not prominently enhanced using blood monocyte-derived macrophages, suggesting that the relevant receptor is expressed on MDDC. Infection of MDDC in the presence of bovine lactoferrin was blocked by mannan, and an antibody to CD209/DC-SIGN but not isotype control or CD46 antibodies. Lastly, U937 macrophages ectopically expressing CD209/DC-SIGN, but not parental U937 cells, were efficiently infected by adenovirus 2C in the presence of bovine lactoferrin. These results may provide a tool, given the high efficiency of infection, to dissect responses by myeloid cells to clinical adenovirus isolates.
authors.affiliation: Institut für Medizinische Virologie und Epidemiologie der Viruserkrankungen, Universitätsklinikum Tübingen, 72076 Tübingen, Germany.
authors.forename: Patrick S
authors.initials: PS
authors.lastname: Günther
authors.affiliation: Institut für Medizinische Virologie und Epidemiologie der Viruserkrankungen, Universitätsklinikum Tübingen, 72076 Tübingen, Germany.
authors.forename: Elfriede
authors.initials: E
authors.lastname: Mikeler
authors.affiliation: Institut für Medizinische Virologie und Epidemiologie der Viruserkrankungen, Universitätsklinikum Tübingen, 72076 Tübingen, Germany.
authors.forename: Klaus
authors.initials: K
authors.lastname: Hamprecht
authors.affiliation: Institut für Virologie und Immunbiologie, Universität Würzburg, 97078 Würzburg, Germany.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Schneider-Schaulies
authors.affiliation: Institut für Medizinische Virologie und Epidemiologie der Viruserkrankungen, Universitätsklinikum Tübingen, 72076 Tübingen, Germany.
authors.forename: Gerhard
authors.initials: G
authors.lastname: Jahn
authors.affiliation: Institut für Medizinische Virologie und Epidemiologie der Viruserkrankungen, Universitätsklinikum Tübingen, 72076 Tübingen, Germany.
authors.forename: Kevin M
authors.initials: KM
authors.lastname: Dennehy
mesh_terms: D000256:Adenoviridae / Q000276:immunology / Q000302:isolation & purification / Q000502:physiology*; D000257:Adenoviridae Infections / Q000276:immunology* / Q000821:virology; D000818:Animals; D002417:Cattle; D015815:Cell Adhesion Molecules / Q000276:immunology*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology* / Q000821:virology; D006801:Humans; D007781:Lactoferrin / Q000276:immunology*; D037181:Lectins, C-Type / Q000276:immunology*; D009000:Monocytes / Q000276:immunology* / Q000821:virology; D011956:Receptors, Cell Surface / Q000276:immunology*; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
chemical_list: D015815:Cell Adhesion Molecules; D007781:Lactoferrin; D037181:Lectins, C-Type; D011956:Receptors, Cell Surface; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin