title: [Phenotypic analysis of BCG-specific effector memory CD4+ T cells in PBMCs by eight-color flow cytometry].
abstract: OBJECTIVE
  To evaluate cytokine production and subsets in PBMCs from PPD+ normal donors after stimulation with BCG.
  
  
  METHODS
  PBMCs were isolated from PPD+ normal individuals, and cytokine production and BCG-specific T cell subsets were assessed by eight-color flow cytometry.
  
  
  RESULTS
  Following stimulation with BCG, CD4+ but not CD8+ T cells expressed IFN-γ, IL-2 and TNF-α. Phenotypic analysis indicated that cytokine-producing cells were CD4+CD45RO+CD62L-CD27- and CD4+CD45RO+CD62L-CD27+.
  
  
  CONCLUSIONS
  BCG predominantly induced CD4+ T cells to produce cytokines following stimulation with BCG. Further analysis indicated that these cells are CD4+CD45RO+CD62L- effector memory cells, suggesting that these cells probably played essential role in preventing TB infection.
authors.affiliation: Institute of Immunology, Zhongshan School of Medicine, Sun Yat-sen University, Key Laboratory of Tropical Disease Control Research of Ministry of Education, Guangzhou 510080, China. ly_li@yahoo.cn
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Xiao-ying
authors.initials: XY
authors.lastname: Fu
authors.forename: Chang-you
authors.initials: CY
authors.lastname: Wu
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis / Q000276:immunology; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D009163:Mycobacterium bovis / Q000276:immunology*
chemical_list: D016207:Cytokines