title: Interaction of the cyclophosphamide metabolite, acrolein, with lactate dehydrogenase-m homolog.
abstract: The cyclophosphamide metabolite, acrolein, binds to both nucleic acids and proteins. In this report, acrolein is shown to interact preferentially with a rat liver 35KD DNA-binding protein. The 35KD protein was purified to homogeneity from a hepatic microsomal fraction using (NH4)2SO4 precipitation, DEAE-Sepharose chromatography, cation exchange HPLC, and DNA-Sepharose affinity chromatography. The protein was identified as a homologue of lactate dehydrogenase (LDH) M subunit on the basis of partial amino acid sequencing, amino acid composition and immunological methods. The interaction of this protein with acrolein may have implications for tissue-specific toxicities and/or oncoselectivity of cyclophosphamide.
authors.affiliation: ROSWELL PK CANC INST,GRACE CANC DRUG CTR,ELM & CARLTON ST,BUFFALO,NY 14263. BURROUGHS WELLCOME CO,RES TRIANGLE PK,NC 27709.
authors.forename: F
authors.initials: F
authors.lastname: Scappaticci
authors.forename: A
authors.initials: A
authors.lastname: Maccubbin
authors.forename: N
authors.initials: N
authors.lastname: Srikanth
authors.forename: M
authors.initials: M
authors.lastname: Faletto
authors.forename: H
authors.initials: H
authors.lastname: Gurtoo