title: Assessment of antimicrobial effect of Biosilicate® against anaerobic, microaerophilic and facultative anaerobic microorganisms.
abstract: This study assessed the antimicrobial activity of a new bioactive glass-ceramic (Biosilicate®) against anaerobic, microaerophilic, and facultative anaerobic microorganisms. Evaluation of the antimicrobial activity was carried out by three methods, namely agar diffusion, direct contact, and minimal inhibitory concentration (MIC). For the agar diffusion technique, bio glass-ceramic activity was observed against various microorganisms, with inhibition haloes ranging from 9.0 ± 1.0 to 22.3 ± 2.1 mm. For the direct contact technique, Biosilicate® displayed activity against all the microorganisms, except for S. aureus. In the first 10 min of contact between the microorganisms and Biosilicate®, there was a drastic reduction in the number of viable cells. Confirming the latter results, MIC showed that the Biosilicate® inhibited the growth of microorganisms, with variations between ≤ 2.5 and 20 mg/ml. The lowest MIC values (7.5 to ≤ 2.5 mg/ml) were obtained for oral microorganisms. In conclusion, Biosilicate® exhibits a wide spectrum of antimicrobial properties, including anaerobic bacteria.
authors.affiliation: Laboratory of Research in Applied Microbiology, University of Franca, Franca, SP 14404-600, Brazil. martinsc@unifran.br
authors.forename: Carlos Henrique Gomes
authors.initials: CH
authors.lastname: Martins
authors.forename: Tatiane Cruz
authors.initials: TC
authors.lastname: Carvalho
authors.forename: Maria Gorete Mendes
authors.initials: MG
authors.lastname: Souza
authors.forename: Christian
authors.initials: C
authors.lastname: Ravagnani
authors.forename: Oscar
authors.initials: O
authors.lastname: Peitl
authors.forename: Edgar Dutra
authors.initials: ED
authors.lastname: Zanotto
authors.forename: Heitor
authors.initials: H
authors.lastname: Panzeri
authors.forename: Luciana Assirati
authors.initials: LA
authors.lastname: Casemiro
mesh_terms: D000362:Agar / Q000494:pharmacology; D000693:Anaerobiosis / Q000187:drug effects / Q000502:physiology; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology*; D001421:Bacteria, Anaerobic / Q000187:drug effects* / Q000254:growth & development; D004058:Diffusion; D005898:Glass / Q000032:analysis / Q000737:chemistry; D008826:Microbial Sensitivity Tests; D008828:Microbiological Techniques; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development
chemical_list: D000890:Anti-Infective Agents; C120461:sodium oxide-calcium oxide-silicon dioxide-diphosphorus pentoxide; D000362:Agar