title: Detection of betaproteobacteria inside the mycelium of the fungus Mortierella elongata.
abstract: Microscopic and molecular analyses showed the presence of endobacteria inside the mycelia of four out of twelve nitrous oxide (N(2)O)-producing fungal isolates identified as Mortierella elongata. The 16S rRNA gene was successfully amplified with DNA extracted directly from the endobacterium-containing fungal strains and all sequences were related to that of Candidatus Glomeribacter gigasporarum in the family Burkholderiaceae. Bacterial endotoxin was detected in the endobacterium-positive fungal strains but only trace levels were found in endobacterium-negative strains. No significant relationship was found between the fungal N(2)O-producing activity and the presence of endobacteria.
authors.affiliation: Institute for Global Change Adaptation Science, Ibaraki University, 2–1–1 Bunkyo, Mito, Ibaraki 310–8512, Japan.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Sato
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Narisawa
authors.forename: Kazuto
authors.initials: K
authors.lastname: Tsuruta
authors.forename: Masafumi
authors.initials: M
authors.lastname: Umezu
authors.forename: Tomoyasu
authors.initials: T
authors.lastname: Nishizawa
authors.forename: Kenji
authors.initials: K
authors.lastname: Tanaka
authors.forename: Kaoru
authors.initials: K
authors.lastname: Yamaguchi
authors.forename: Masakazu
authors.initials: M
authors.lastname: Komatsuzaki
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Ohta
mesh_terms: D020562:Betaproteobacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology*; D008969:Molecular Sequence Data; D020094:Mortierella / Q000302:isolation & purification / Q000502:physiology*; D025282:Mycelium / Q000502:physiology; D009609:Nitrous Oxide; D010802:Phylogeny; D012988:Soil Microbiology; D013559:Symbiosis
chemical_list: D009609:Nitrous Oxide