title: Inhibitory effects of secoiridoids from the roots of Gentiana straminea on stimulus-induced superoxide generation, phosphorylation and translocation of cytosolic compounds to plasma membrane in human neutrophils.
abstract: Gentiana straminea Maxim. has been used widely as a traditional Chinese medicine for the treatment of rheumarthritis, icterepatitis, constipation, pain and hypertension. Five secoiridoids, gentiopicroside (GTP), 6'-O-(2-hydroxy-3-O-β-D-glucopyranosyl-benzoyl)-sweroside (HGBS), 6'-O-β-D-glucosylgentiopicroside (GGTP), sweroside (SW) and swertiamarin (STM) were isolated from the roots of G. straminea. The effect of these secoiridoids on stimulus-induced superoxide generation in human neutrophils was assayed by measuring the reduction of ferricytochrome c. Tyrosyl or serine/threonine phosphorylation of neutrophil proteins, and translocation of the cytosolic compounds to the cell membrane were also investigated using specific monoclonal antibodies. The five secoiridoids used in the present experiment suppressed N-formyl-methionyl-leucyl-phenylalanine (fMLP)-induced superoxide generation in a concentration dependent manner. GTP and HGBS also suppressed phorbol 12-myristate 13-acetate (PMA) and arachidonic acid (AA)-induced superoxide generation. However, the other three secoiridoids showed no effect on PMA- and AA-induced superoxide generation. fMLP-, PMA- and AA-induced tyrosyl or serine/threonine phosphorylation and translocation of the cytosolic proteins to the cell membrane were suppressed in parallel with the suppression of the stimulus-induced superoxide generation.
authors.affiliation: Department of Anesthesiology and Critical Care Medicine, Kochi Medical School, Nankoku-Shi, Kochi 783-8505, Japan.
authors.forename: Shihu
authors.initials: S
authors.lastname: Wei
authors.forename: Guang
authors.initials: G
authors.lastname: Chen
authors.forename: Wenfei
authors.initials: W
authors.lastname: He
authors.forename: Haidong
authors.initials: H
authors.lastname: Chi
authors.forename: Hideki
authors.initials: H
authors.lastname: Abe
authors.forename: Koichi
authors.initials: K
authors.lastname: Yamashita
authors.forename: Masanori
authors.initials: M
authors.lastname: Yokoyama
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kodama
mesh_terms: D016718:Arachidonic Acid; D002462:Cell Membrane / Q000378:metabolism*; D002478:Cells, Cultured; D030012:Gentiana / Q000737:chemistry*; D006801:Humans; D057888:Iridoid Glucosides / Q000494:pharmacology; D039823:Iridoids / Q000494:pharmacology*; D009240:N-Formylmethionine Leucyl-Phenylalanine; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D010766:Phosphorylation; D018517:Plant Roots / Q000737:chemistry; D021381:Protein Transport / Q000187:drug effects; D011753:Pyrones / Q000494:pharmacology; D013481:Superoxides / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate
chemical_list: C516875:6'-O-beta-D-glucosylgentiopicroside; D057888:Iridoid Glucosides; D039823:Iridoids; D011753:Pyrones; C012997:gentiopicroside; D013481:Superoxides; D016718:Arachidonic Acid; C013270:swertiamarin; D009240:N-Formylmethionine Leucyl-Phenylalanine; C049412:sweroside; D013755:Tetradecanoylphorbol Acetate