title: [A study of frequency of TNF alpha gene with type 2 diabetes mellitus with chronic periodontitis].
abstract: OBJECTIVE
  To detect the frequency of TNF alpha gene in patients of type 2 diabetes mellitus with chronic periodontitis, periodontitis without any systemic diseases and healthy controls.
  
  
  METHODS
  The case series were consisted of 112 patients with moderate, severe type 2 diabetes mellitus with chronic periodontitis, 99 patients with moderate, severe periodontitis without any systemic disease, 50 age- and gender-matched subjects with healthy periodontal conditions were enrolled. Clinical parameters were measured and recorded including probing depth(PD), clinical attachment loss(CAL), bleeding index(BI), and tooth movement(TM). The polymorphism of TNF-α-308 genotype (TNF1/2) was examined after electrophoresis on agarose gel and ethidium bromide staining. The difference between the case and healthy groups was analysed by Chi-square test, the difference in clinical index among groups which had different allele was analyzed for ANOVA with SPSS13.0 software package.
  
  
  RESULTS
  We divided DM and CP groups into moderate and severe groups. There were significant difference between severe DM group and severe, moderate CP group, moderate DM group and chronic periodontitis of severe,moderate group. The probing depth and clinical attachment loss of the patients who took TNF-α-308 allele II were significantly higher than the patients who took TNF-α-308 allele I in DM and CP group.
  
  
  CONCLUSIONS
  TNF-α-308 allele II might increase the susceptivity of periodontitis in population. TNF-α-308 allele II may play an important role in synergistic reaction of periodontitis and type 2 diabetes.
authors.affiliation: Department of Periodontology, School of Stomatology, China Medical University, Shenyang 110002, Liaoning Province, China. lba555@163.com
authors.forename: Bo
authors.initials: B
authors.lastname: Liu
authors.forename: Ning
authors.initials: N
authors.lastname: Yu
authors.forename: Li-si
authors.initials: LS
authors.lastname: Tan
authors.forename: Jing-bo
authors.initials: JB
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Guo
authors.forename: Ya-ping
authors.initials: YP
authors.lastname: Pan
mesh_terms: D000483:Alleles; D055113:Chronic Periodontitis; D003924:Diabetes Mellitus, Type 2; D005838:Genotype; D006801:Humans; D010510:Periodontal Diseases; D010518:Periodontitis; D011110:Polymorphism, Genetic; D014409:Tumor Necrosis Factor-alpha
chemical_list: D014409:Tumor Necrosis Factor-alpha