title: Analysis of omega 3 fatty acid in natural and enriched chicken eggs by capillary zone electrophoresis.
abstract: Qualitative differentiation between natural and enriched chicken eggs through omega (ω) 3 fatty acid profiles by capillary zone electrophoresis (CZE) under direct UV detection at 200 nm is proposed. The electrolyte background consisted of 12.0 mmol L(-1) tetraborate buffer (pH 9.2) mixed with 12.0 mmol L(-1) Brij 35, 17% acetonitrile (ACN) and 33% methanol (MeOH). Omega 3 fatty acid profile in chicken egg samples were analyzed by CZE system and confirmed by single-quadrupole mass spectrometry with an electrospray ionization probe set to negative ionization mode after sample preparation by the Folch method. The results showed that ω fatty acid profiles analyzed by the CZE approach can be used to chemical markers to monitor fraud, presenting simplicity, short analysis time (10 min) and low cost as advantages.
authors.affiliation: Departamento de Química, Instituto de Ciências Exatas, Universidade Federal de Juiz de Fora, Cidade Universitária, MG, Brazil.
authors.forename: Brenda Lee Simas
authors.initials: BL
authors.lastname: Porto
authors.forename: Marcus Vinicius Nora de
authors.initials: MV
authors.lastname: Souza
authors.forename: Marcone Augusto Leal de
authors.initials: MA
authors.lastname: Oliveira
mesh_terms: D000818:Animals; D002645:Chickens; D004531:Eggs / Q000032:analysis*; D019075:Electrophoresis, Capillary; D015525:Fatty Acids, Omega-3 / Q000032:analysis*; D015394:Molecular Structure; D013056:Spectrophotometry, Ultraviolet
chemical_list: D015525:Fatty Acids, Omega-3