title: Structural investigation and biological activity of the lipooligosaccharide from the psychrophilic bacterium Pseudoalteromonas haloplanktis TAB 23.
abstract: Pseudoalteromonas haloplanktis TAB 23 is a Gram-negative psychrophilic bacterium isolated from the Antarctic coastal sea. To survive in these conditions psychrophilic bacteria have evolved typical membrane lipids and "antifreeze" proteins to protect the inner side of the microorganism. As for Gram-negative bacteria, the outer membrane is mainly constituted by lipopoly- or lipooligosaccharides (LPS or LOS, respectively), which lean towards the external environment. Despite this, very little is known about the peculiarity of LPS from Gram-negative psychrophilic bacteria and what their role is in adaptation to cold temperature. Here we report the complete structure of the LOS from P. haloplanktis TAB 23. The lipid A was characterized by MALDI-TOF MS analysis and was tested in vitro showing a significant inhibitory effect on the LPS-induced pro-inflammatory cytokine production when added in culture with LPS from Escherichia coli. The product obtained after de-O-acylation of the LPS was analyzed by MALDI-TOF MS revealing the presence of several molecular species, differing in phosphorylation degree and oligosaccharide length. The oligosaccharide portion released after strong alkaline hydrolysis was purified by anion-exchange chromatography-pulsed amperometric detection (HPAEC-PAD) to give three main fractions, characterized by means of 2D NMR spectroscopy, which showed a very short highly phosphorylated saccharidic chain with the following general structure. α-Hepp3R,6R,4R'-(1→5)-α-Kdop4P-(2→6)-β-GlcpN4R-(1→6)-α-GlcpN1P (R=-H(2)PO(3) or -H; R'=α-Galp-(1→4)-β-Galp-(1→ or H-).
authors.affiliation: Dipartimento di Chimica Organica e Biochimica, Università Federico II di Napoli, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Napoli, Italia.
authors.forename: Sara
authors.initials: S
authors.lastname: Carillo
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Pieretti
authors.forename: Ermenegilda
authors.initials: E
authors.lastname: Parrilli
authors.forename: Maria L
authors.initials: ML
authors.lastname: Tutino
authors.forename: Sabrina
authors.initials: S
authors.lastname: Gemma
authors.forename: Monica
authors.initials: M
authors.lastname: Molteni
authors.forename: Rosa
authors.initials: R
authors.lastname: Lanzetta
authors.forename: Michelangelo
authors.initials: M
authors.lastname: Parrilli
authors.forename: Maria M
authors.initials: MM
authors.lastname: Corsaro
mesh_terms: D000864:Antarctic Regions; D002236:Carbohydrate Conformation; D003080:Cold Temperature; D004926:Escherichia coli / Q000737:chemistry*; D008050:Lipid A / Q000737:chemistry* / Q000302:isolation & purification; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D008956:Models, Chemical; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry*; D031161:Pseudoalteromonas / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D008050:Lipid A; D008070:Lipopolysaccharides; D009844:Oligosaccharides; C023023:lipid-linked oligosaccharides