title: Novel nuclear intron-spanning primers for Arecaceae evolutionary biology.
abstract: In this study, 96 nuclear 'conserved intron-scanning primers' were screened across subfamilies the Arecaceae (palms) for potential use in research focused on palm evolutionary biology. Primers were evaluated based on their ability to amplify single polymerase chain reaction products in Arecaceae, the clarity of sequencing reads, and the interspecific variability observed. Ultimately, the results suggest that: (i) seven of the loci are likely to be suitable when comparing non-Arecaceae outgroups and Arecaceae ingroups; (ii) seven loci may be of use when comparing subfamilies of Arecaceae; and (iii) four of the loci may be of use when comparing closely related genera.
authors.affiliation: Department of Biology, MSC 3AF, New Mexico State University, PO Box 30001, Las Cruces, NM 88003, USA, Plant Genome Mapping Laboratory, University of Georgia, 111 Riverbend Road, Athens, GA 30602, USA.
authors.forename: Christine D
authors.initials: CD
authors.lastname: Bacon
authors.forename: F Alex
authors.initials: FA
authors.lastname: Feltus
authors.forename: Andrew H
authors.initials: AH
authors.lastname: Paterson
authors.forename: C Donovan
authors.initials: CD
authors.lastname: Bailey