title: Headspace solid-phase microextraction with on-fiber derivatization for the determination of aldehydes in algae by gas chromatography-mass spectrometry.
abstract: A simple, fast, sensitive and cost-effective method based on headspace solid-phase microextraction (HS-SPME) with on-fiber derivatization coupled with gas chromatography-mass spectrometry was developed for the determination of six typical aldehydes, 2E-hexenal, heptanal, 2E-heptenal, 2E,4E-heptadienal, 2E-decenal and 2E,4E-decadienal in laboratory algae cultures. As derivatization reagent, O-2,3,4,5,6-(pentafluorobenzyl) hydroxylamine hydrochloride, was loaded onto the poly(dimethylsiloxane)/divinylbenzene fiber for aldehydes on-fiber derivatization prior to HS-SPME. Various influence factors of extraction efficiency were systematically investigated. Under optimized extraction conditions, excellent method performances for all the six aldehydes were attained, such as satisfactory extraction recoveries ranging from 67.1 to 117%, with the precision (relative standard deviation) within 5.3-11.1%, and low detection limits in the range of 0.026-0.044 μg/L. The validated method was successfully applied for the analysis of the aldehydes in two diatoms (Skeletonema costatum and Chaetoceros muelleri), two pyrrophytas (Prorocentrum micans and Scrippsiella trochoidea) and Calanus sinicus eggs (feeding on the two diatoms above).
authors.affiliation: Key Laboratory of Environmental Engineering of Shandong Province, Institute of Environment and Municipal Engineering, Qingdao Technological University, Qingdao, PR China. majiping2001@yahoo.com.cn
authors.forename: Jiping
authors.initials: J
authors.lastname: Ma
authors.forename: Ronghui
authors.initials: R
authors.lastname: Xiao
authors.forename: Jinhua
authors.initials: J
authors.lastname: Li
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.forename: Benzhang
authors.initials: B
authors.lastname: Shi
authors.forename: Yanjuan
authors.initials: Y
authors.lastname: Liang
authors.forename: Wenhui
authors.initials: W
authors.lastname: Lu
authors.forename: Lingxin
authors.initials: L
authors.lastname: Chen
mesh_terms: D000447:Aldehydes / Q000032:analysis* / Q000302:isolation & purification; D017377:Diatoms / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D052617:Solid Phase Microextraction / Q000379:methods*
chemical_list: D000447:Aldehydes