title: Regulation of basophilic and erythroid-differentiation of a human chronic myelogenous leukemia-cell line, ku812f, by interleukin-3 and stem-cell factor.
abstract: We have investigated the effects of c-kit ligand (stem cell factor [SCF]) and interleukin-3 (IL-3) on proliferation and differentiation of a human chronic myelogenous leukemia cell line, KU812F, which can differentiate toward erythroid and basophilic lineages. When purified c-kit-positive cells (approximately 20% of KU812F cells) were used as a target, SCF induced not only proliferation but also augumented erythroid differentiation of the cells, while IL-3 did promote basophilic differentiation. Further, analyses of in situ hybridization and cell sorting with anti-c-kit antibody showed that the expression of c-kit decreased along with differentiation from immature to mature basophils and erythroid cells.
authors.affiliation: OSAKA UNIV,SCH MED,DEPT INTERNAL MED 2,1-1-50 FUKUSHIMA,FUKUSHIMA KU,OSAKA 553,JAPAN. OSAKA UNIV,SCH MED,DEPT PATHOL,OSAKA 553,JAPAN. OSAKA UNIV,SCH MED,DEPT PHARMACOL,OSAKA 553,JAPAN.
authors.forename: H
authors.initials: H
authors.lastname: Kitayama
authors.forename: Y
authors.initials: Y
authors.lastname: Kanakura
authors.forename: T
authors.initials: T
authors.lastname: Furitsu
authors.forename: H
authors.initials: H
authors.lastname: Ikeda
authors.forename: S
authors.initials: S
authors.lastname: Hirota
authors.forename: H
authors.initials: H
authors.lastname: Sugahara
authors.forename: A
authors.initials: A
authors.lastname: Yamatodani
authors.forename: Y
authors.initials: Y
authors.lastname: Kanayama
authors.forename: Y
authors.initials: Y
authors.lastname: Kitamura
authors.forename: Y
authors.initials: Y
authors.lastname: Matsuzawa