title: PCR-RFLP to detect codon 248 mutation in exon 7 of p53 tumor suppressor gene.
abstract: Individual genome DNA was extracted fast from oral swab and followed up with PCR specific for codon 248 of p53 tumor suppressor gene. MspI restriction mapping showed the G-C mutation in codon 248, which closely relates to cancer susceptibility. Students learn the concepts, detection techniques, and research significance of point mutations or single nucleotide polymorphisms in individual genomes.
authors.affiliation: Department of Applied biology, School of Biotechnology, East China University of Science and Technology, Shanghai 200237, People's Republic of China.
authors.forename: Liming
authors.initials: L
authors.lastname: Ouyang
authors.forename: Chongtao
authors.initials: C
authors.lastname: Ge
authors.forename: Haizhen
authors.initials: H
authors.lastname: Wu
authors.forename: Suxia
authors.initials: S
authors.lastname: Li
authors.forename: Huizhan
authors.initials: H
authors.lastname: Zhang