title: GABAergic synaptic response and its opioidergic modulation in periaqueductal gray neurons of rats with neuropathic pain.
abstract: BACKGROUND
  Neuropathic pain is a chronic and intractable symptom associated with nerve injury. The periaqueductal gray (PAG) is important in the endogenous pain control system and is the main site of the opioidergic analgesia. To investigate whether neuropathic pain affects the endogenous pain control system, we examined the effect of neuropathic pain induced by sacral nerve transection on presynaptic GABA release, the kinetics of postsynaptic GABA-activated Cl- currents, and the modulatory effect of μ-opioid receptor (MOR) activation in mechanically isolated PAG neurons with functioning synaptic boutons.
  
  
  RESULTS
  In normal rats, MOR activation inhibited the frequency of GABAergic miniature inhibitory postsynaptic currents (mIPSCs) to 81.3% of the control without any alteration in their amplitude. In neuropathic rats, the inhibition of mIPSC frequency by MOR activation was 82.4%. The frequency of GABAergic mIPSCs in neuropathic rats was 151.8% of normal rats without any difference in the mIPSC amplitude. Analysis of mIPSC kinetics showed that the fast decay time constant and synaptic charge transfer of mIPSCs in neuropathic rats were 76.0% and 73.2% of normal rats, respectively.
  
  
  CONCLUSIONS
  These results indicate that although the inhibitory effect of MOR activation on presynaptic GABA release is similar in both neuropathic and normal rats, neuropathic pain may inhibit endogenous analgesia in the PAG through an increase in presynaptic GABA release.
authors.affiliation: Department of Physiology, Biomedical Science Institute, Kyung Hee University School of Medicine, Seoul 130-701, South Korea.
authors.forename: Eu-Teum
authors.initials: ET
authors.lastname: Hahm
authors.forename: Younghoon
authors.initials: Y
authors.lastname: Kim
authors.forename: Jong-Ju
authors.initials: JJ
authors.lastname: Lee
authors.forename: Young-Wuk
authors.initials: YW
authors.lastname: Cho
mesh_terms: D000701:Analgesics, Opioid / Q000378:metabolism*; D000818:Animals; D053444:Inhibitory Postsynaptic Potentials / Q000502:physiology; D008297:Male; D055369:Miniature Postsynaptic Potentials / Q000502:physiology; D009437:Neuralgia / Q000378:metabolism* / Q000503:physiopathology; D009474:Neurons / Q000502:physiology*; D010487:Periaqueductal Gray / Q000378:metabolism* / Q000503:physiopathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013569:Synapses / Q000502:physiology*; D009435:Synaptic Transmission / Q000502:physiology; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
chemical_list: D000701:Analgesics, Opioid; D005680:gamma-Aminobutyric Acid