title: Isolation and characterization of 64 novel microsatellite markers from a fosmid library of female half-smooth tongue sole (Cynoglossus semilaevis).
abstract: Microsatellite markers were developed from a fosmid library of female half-smooth tongue sole, Cynoglossus semilaevis. Three hundred eighty-four clones were randomly selected to sequence (double strand reading), and 168 sequences in 143 clones were found to contain microsatellites. Of the 101 primer pairs designed, 64 gave polymorphic polymerase chain reaction products. Based on characterization with 36 individuals, the number of alleles ranged from two to nine. The values of observed and expected heterozygosities varied from 0.06 to 1.00 and from 0.05 to 0.94, respectively. These markers have the potential as tools for population structure evaluation, ecological analyses and linkage map construction.
authors.affiliation: Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.
authors.forename: Xubo
authors.initials: X
authors.lastname: Wang
authors.forename: Quanqi
authors.initials: Q
authors.lastname: Zhang
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Sun
authors.forename: Guoliang
authors.initials: G
authors.lastname: Yin
authors.forename: Jie
authors.initials: J
authors.lastname: Qi
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Wang
authors.forename: Xinglian
authors.initials: X
authors.lastname: Wang