title: Neuropeptide Y overflow and metabolism in skeletal muscle arterioles.
abstract: The purpose of this study was to characterize neuropeptide Y (NPY) overflow and metabolism from isolated skeletal muscle arterioles of female rats. Gastrocnemius first-order arterioles were removed from young (2 months), young adult (6 months) and middle-aged (12 months) F344 female rats. Arterioles were isolated, cannulated and pressurized in a microvessel bath with field stimulation electrodes. NPY overflow from isolated arterioles was assessed at 0 s and 30 s post-field stimulation. Dipeptidyl peptidase IV (DPPIV) activity was quantified via fluorometric assay of whole vessel homogenate. In young adult and middle-aged rats, NPY overflow increased 0 s and 30 s following field stimulation. In young adult rats, DPPIV inhibition resulted in an increase in NPY overflow at 30 s, while middle-aged rats had no increase in NPY overflow with DPPIV inhibition (P <0.05). DPPIV activity was influenced by factors such as age, vessel type, and endothelium (P <0.05). The present data suggest that DPPIV plays a significant role in modulating the actions of NPY in arterioles of young adult females; however, this role appears to diminish with age.
authors.affiliation: Department of Health Science, Kinesiology, Recreation and Dance, University of Arkansas, Fayetteville, AR, USA.
authors.forename: Kirk W
authors.initials: KW
authors.lastname: Evanson
authors.forename: Audrey J
authors.initials: AJ
authors.lastname: Stone
authors.forename: Allyson L
authors.initials: AL
authors.lastname: Hammond
authors.forename: Heidi A
authors.initials: HA
authors.lastname: Kluess
mesh_terms: D000375:Aging / Q000502:physiology; D000818:Animals; D001160:Arterioles / Q000201:enzymology / Q000378:metabolism / Q000502:physiology*; D018819:Dipeptidyl Peptidase 4 / Q000502:physiology; D005260:Female; D008297:Male; D055442:Metabolome / Q000502:physiology; D018482:Muscle, Skeletal / Q000098:blood supply* / Q000201:enzymology / Q000378:metabolism*; D009478:Neuropeptide Y / Q000378:metabolism / Q000502:physiology*; D051381:Rats; D011916:Rats, Inbred F344
chemical_list: D009478:Neuropeptide Y; D018819:Dipeptidyl Peptidase 4