title: Prostaglandin E(2) binding peptide screened by phage displaying: a new therapeutic strategy in rheumatoid arthritis.
abstract: OBJECTIVE
  To investigate the therapeutic potential and mechanism of action of the mimotope of PGE(2) receptor EP4 (PBP, named by our team) screened by phage displaying technique in the treatment of adjuvant-induced arthritis (AA).
  
  
  METHODS
  Freund's complete adjuvant-induced arthritis was induced in Wistar rats. At the first clinical sign of disease, mice were given with daily injections of PBP or saline for 21 days. Disease progression was monitored by measurement of paw swelling. Inflammation and joint destruction were assessed histologically. The IL-1β and TNF-α were studied by ELISA in the ankle steeps of arthritis model. The degree of proliferation and apoptosis of synoviocytes of RA patients were assessed by CCK-8 kit and AnnexinV-FITC/PI respectively.
  
  
  RESULTS
  PBP-treated animals displayed significantly less cartilage and bone destruction than model controls. Tumor necrosis factor α and IL-1β expression were reduced after PBP treatment. The proliferation and apoptosis of synoviocytes of RA patients were influenced by PBP.
  
  
  CONCLUSIONS
  The data support the view that PBP is a potential therapy for RA that may help to diminish both joint inflammation and destruction. And the activities of PBP are related with the effect on synoviocytes directly.
authors.affiliation: Department of Immunology, Norman Bethune College of Medicine, Jilin University, Changchun, China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Yan
authors.forename: Weiwei
authors.initials: W
authors.lastname: Han
authors.forename: Qinzhu
authors.initials: Q
authors.lastname: Bai
authors.forename: Xiangfeng
authors.initials: X
authors.lastname: Zhao
authors.forename: Xiao
authors.initials: X
authors.lastname: Han
authors.forename: Bairong
authors.initials: B
authors.lastname: Du
authors.forename: Xun
authors.initials: X
authors.lastname: Zhu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001169:Arthritis, Experimental / Q000473:pathology; D001172:Arthritis, Rheumatoid / Q000473:pathology / Q000628:therapy*; D002470:Cell Survival / Q000187:drug effects; D015232:Dinoprostone / Q000737:chemistry / Q000378:metabolism*; D005260:Female; D006801:Humans; D007249:Inflammation / Q000473:pathology; D053583:Interleukin-1beta / Q000378:metabolism; D007596:Joints / Q000187:drug effects / Q000473:pathology; D008297:Male; D051379:Mice; D018613:Microscopy, Confocal; D008875:Middle Aged; D008958:Models, Molecular; D019151:Peptide Library; D010455:Peptides / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D011485:Protein Binding / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D013583:Synovial Membrane / Q000187:drug effects / Q000473:pathology; D013643:Tarsus, Animal / Q000187:drug effects / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D053583:Interleukin-1beta; D019151:Peptide Library; D010455:Peptides; D014409:Tumor Necrosis Factor-alpha; D015232:Dinoprostone