title: Removal of arylamine-DNA adducts in cultured epithelial-cells of rat and human urinary-bladder and mammary-gland.
abstract: Primary mammary and urinary bladder epithelial cells of the rat, and the human urothelial cell line HCV-29 and mammary cell line MCF-10A were incubated for 3 h with 10 mM hydroxyurea and N-hydroxy-N-acetyl-2-aminofluorene, N-hydroxy-N-acetyl-4-aminobiphenyl or N-hydroxy-3,2'-dimethyl-4-aminobiphenyl. DNA adducts of these carcinogens were detected in the nuclei of all cells, except MCF-10A, semi-quantitatively with a novel immunohistochemical-microdensitometric method. The loss of these adducts in these cells was biphasic and was relatively greater between 0 and 24 h than between 24 and 48 h. The half-life of the adducts of all 3 carcinogens was approximately 20 h. The adduct loss is consistent with repair detected by an unscheduled DNA synthesis system reported previously. These results demonstrate that nonacetylated aromatic amine-DNA adducts can be repaired in the target cells for aromatic amine-induced carcinogenesis.
authors.affiliation: MICHIGAN CANC FDN,DEPT CHEM CARCINOGENESIS,DETROIT,MI 48201. AICHI CANC CTR,RES INST,BIOCHEM LAB,NAGOYA,AICHI 464,JAPAN. NCI,CELLULAR CARCINOGENESIS & TUMOR PROMOT LAB,BETHESDA,MD 20892.
authors.forename: M
authors.initials: M
authors.lastname: Debiecrychter
authors.forename: K
authors.initials: K
authors.lastname: Zukowski
authors.forename: M
authors.initials: M
authors.lastname: Tada
authors.forename: M
authors.initials: M
authors.lastname: Poirier
authors.forename: C
authors.initials: C
authors.lastname: Wang