title: Modulatory activities of Agelanthus dodoneifolius (Loranthaceae) extracts on stimulated equine neutrophils and myeloperoxidase activity.
abstract: Agelanthus dodoneifolius DC Danser (Loranthaceae) is used for the treatment of various diseases including asthma. The aqueous and hydroalcoholic extracts have been reported to have anti-inflammatory, spasmolytic and bronchorelaxant activities. The present study investigates the effects of the aqueous decoction and the diethyl ether, ethyl acetate and butanolic fractions of Agelanthus dodoneifolius DC Danser (Loranthaceae) on reactive oxygen species (ROS) production and myeloperoxidase (MPO) release by phorbol 12-myristate 13-acetate (PMA)-stimulated equine neutrophils and on purified equine MPO activity. ROS production and MPO release by the PMA-stimulated neutrophils were measured by the lucigenin-enhanced chemiluminescence and ELISA assays, respectively. Specific immunological extraction followed by enzymatic detection (SIEFED) was used to specifically measure the equine MPO activity. Identification and quantification of the individual and total phenolic and flavonoid compounds were performed using UPLC-MS/MS equipment and colorimetric methods involving Folin-Ciocalteu and AlCl₃, respectively. All the tested extracts displayed dose-dependent inhibitory effects on the oxidant activities of neutrophils; a stronger effect was observed with the organic fractions than the aqueous decoction. These findings could be correlated with a high content of phenolic and flavonoid compounds. The results confirm the previously shown anti-inflammatory effect of Agelanthus dodoneifolius and its potential use for the treatment of neutrophil-dependent inflammatory diseases.
authors.affiliation: Laboratory of Bioanalytical Chemistry, Toxicology and Applied Physical Chemistry, Institute of Pharmacy, Free University of Brussels, Campus de la Plaine, CP 205/01, Boulevard du Triomphe, B-1050 Brussels, Belgium. rainboly@ulb.ac.be
authors.forename: Raïnatou
authors.initials: R
authors.lastname: Boly
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Dessy
authors.forename: Stephan
authors.initials: S
authors.lastname: Kohnen
authors.forename: Félix
authors.initials: F
authors.lastname: Kini
authors.forename: Marius
authors.initials: M
authors.lastname: Lompo
authors.forename: Ange
authors.initials: A
authors.lastname: Mouithys-Mickalad
authors.forename: Innocent Pierre
authors.initials: IP
authors.lastname: Guissou
authors.forename: Jacques
authors.initials: J
authors.lastname: Dubois
authors.forename: Ginette
authors.initials: G
authors.lastname: Deby-Dupont
authors.forename: Didier
authors.initials: D
authors.lastname: Serteyn
authors.forename: Thierry
authors.initials: T
authors.lastname: Franck
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology; D006736:Horses; D028183:Loranthaceae / Q000737:chemistry*; D018375:Neutrophil Activation / Q000187:drug effects; D009504:Neutrophils / Q000187:drug effects* / Q000201:enzymology; D009195:Peroxidase / Q000378:metabolism*; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D009195:Peroxidase