title: Secolignans with antiangiogenic activities from Peperomia dindygulensis.
abstract: Two new secolignans, peperomins G and H (1 and 2, resp.), were isolated from the whole plant of Peperomia dindygulensis, together with five known secolignans, peperomin A (3), peperomin E (4), peperomin B (5), 2,3-trans-2-methyl-3-{(3-hydroxy-4,5-dimethoxyphenyl)[5-methoxy-3,4-(methylenedioxy)phenyl]methyl}butyrolactone (6), 2,3-cis-2-(hydroxymethyl)-3-{bis[5-methoxy-3,4-(methylenedioxy)phenyl]methyl}butyrolactone (7). Their structures and configurations were elucidated by spectroscopic methods including 2D-NMR techniques. Antiangiogenic effects of all compounds were evaluated using human umbilical vein endothelial cells (HUVEC) proliferation and tube-formation tests, with compounds 4 and 5 being active in the bioassay. Compounds 4 and 5 induced obvious cell toxicity to HUVEC with IC(50) values of 1.64±0.19 and 8.44±0.4 μM, respectively. Compounds 4 and 5 also exhibited significant HUVEC tube formation-inhibiting activity with IC(50) values of 3.13±0.09 and 6.24±0.12 μM, respectively.
authors.affiliation: Department of Traditional Chinese Medicine, Shanghai Institute of Pharmaceutical Industry, Shanghai, P. R. China.
authors.forename: Meng-Gan
authors.initials: MG
authors.lastname: Lin
authors.forename: De-Hong
authors.initials: DH
authors.lastname: Yu
authors.forename: Qi-Wei
authors.initials: QW
authors.lastname: Wang
authors.forename: Qin
authors.initials: Q
authors.lastname: Lu
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Zhu
authors.forename: Fan
authors.initials: F
authors.lastname: Bai
authors.forename: Gui-Xiu
authors.initials: GX
authors.lastname: Li
authors.forename: Xiang-Wen
authors.initials: XW
authors.lastname: Wang
authors.forename: Yi-Fang
authors.initials: YF
authors.lastname: Yang
authors.forename: Xue-Mei
authors.initials: XM
authors.lastname: Qin
authors.forename: Chao
authors.initials: C
authors.lastname: Fang
authors.forename: Hong-Zhuan
authors.initials: HZ
authors.lastname: Chen
authors.forename: Guo-Hong
authors.initials: GH
authors.lastname: Yang
mesh_terms: D020533:Angiogenesis Inhibitors / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D017705:Lignans / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D036941:Peperomia / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D020533:Angiogenesis Inhibitors; D017705:Lignans; D010936:Plant Extracts