title: Syndecan-2 cytoplasmic domain regulates colon cancer cell migration via interaction with syntenin-1.
abstract: The cell surface heparan sulfate proteoglycan, syndecan-2, is crucial for the tumorigenic activity of colon cancer cells. However, the role played by the cytoplasmic domain of the protein remains unclear. Using colon cancer cells transfected with various syndecan-2-encoding genes with deletions in the cytoplasmic domain, it was shown that syndecan-2-induced migration activity requires the EFYA sequence of the C-terminal region; deletion of these residues abolished the rise in cell migration seen when the wild-type gene was transfected and syndecan-2 interaction with syntenin-1, suggesting that syntenin-1 functioned as a cytosolic signal effector downstream from syndecan-2. Colon cancer cells transfected with the syntenin-1 gene showed increased migratory activity, whereas migration was decreased in cells in which syntenin-1 was knock-down using small inhibitory RNA. In addition, syntenin-1 expression potentiated colon cancer cell migration induced by syndecan-2, and syndecan-2-mediated cell migration was reduced when syntenin-1 expression diminished. However, syntenin-1-mediated migration enhancement was not noted in colon cancer cells transfected with a gene encoding a syndecan-2 mutant lacking the cytoplasmic domain. Furthermore, in line with the increase in cell migration, syntenin-1 mediated Rac activation stimulated by syndecan-2. Together, the data suggest that the cytoplasmic domain of syndecan-2 regulates colon cancer cell migration via interaction with syntenin-1.
authors.affiliation: Department of Life Sciences, Division of Life and Pharmaceutical Sciences, Center for Cell Signaling and Drug Discovery Research, Ewha Womans University, Seoul, Republic of Korea.
authors.forename: Hawon
authors.initials: H
authors.lastname: Lee
authors.forename: Yeonhee
authors.initials: Y
authors.lastname: Kim
authors.forename: Youngsil
authors.initials: Y
authors.lastname: Choi
authors.forename: Sojoong
authors.initials: S
authors.lastname: Choi
authors.forename: Eunkyung
authors.initials: E
authors.lastname: Hong
authors.forename: Eok-Soo
authors.initials: ES
authors.lastname: Oh
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology*; D003593:Cytoplasm / Q000378:metabolism; D006801:Humans; D017434:Protein Structure, Tertiary; D017384:Sequence Deletion; D053669:Syndecan-2 / Q000235:genetics / Q000378:metabolism*; D053676:Syntenins / Q000378:metabolism*
chemical_list: C508245:SDC2 protein, human; C109621:SDCBP protein, human; D053676:Syntenins; D053669:Syndecan-2