title: [Preparation and characterization of the anti-HCV HVR1 synthetic peptide monoclonal antibody].
abstract: OBJECTIVE
  To prepare monoclonal antibody (mAb) against HCV HVR1 synthetic peptide and characterize its property.
  
  
  METHODS
  8 week-old female BALB/c mice were immunized with HCV HVR1 synthetic peptide which was linked to bovine serum albumin (BSA) as immunogen. The splenocytes of immunized mice were fused with Sp2/0 cells by hybridoma technique. Monoclonal hybridoma cell line was obtained by limiting dilution, HAT and HT -selective culture. The specificity of mAb was identified by indirect ELISA, immunohistochemical staining and inhibitory reactivity.
  
  
  RESULTS
  One hybridoma cell (1A9G9F11) was obtained. The mAb belonged to Ig G3. The titers of mAb in ascetic and the relative affinity constant of mAb were 3.125×10(-5) and 1.0×10(6) L/mol respectively.Indirect ELISA detecition demonstrated that the mAb had no cross-reactivates with other viral antigens, such as HBsAg, HBeAg and HAAg, and with other protein, such as BSA, casein and thymopentin.
  
  
  CONCLUSIONS
  One hybridoma cell secreting mAb against-HCV HVR1 has been established successfully, which lays the foundation for for the further study.
authors.affiliation: Department of Immunology, Institute of Infections Diseases, 302nd Hospital of Chinese PLA, Beijing 100039, China. Energysj@yeah.net
authors.forename: Jie
authors.initials: J
authors.lastname: Sun
authors.forename: Yang
authors.initials: Y
authors.lastname: Qi
authors.forename: Shu-ping
authors.initials: SP
authors.lastname: Chi
authors.forename: Li-yue
authors.initials: LY
authors.lastname: You
authors.forename: Rui-sheng
authors.initials: RS
authors.lastname: Li
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
authors.forename: Lei
authors.initials: L
authors.lastname: Zhu
authors.forename: Yun
authors.initials: Y
authors.lastname: Cheng
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology* / Q000302:isolation & purification; D000918:Antibody Specificity / Q000276:immunology; D005260:Female; D018937:Hepatitis C Antibodies / Q000096:biosynthesis* / Q000276:immunology* / Q000302:isolation & purification; D051379:Mice; D008807:Mice, Inbred BALB C; D014764:Viral Proteins / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; C400562:HVR1 protein, Hepatitis C virus; D018937:Hepatitis C Antibodies; D014764:Viral Proteins