title: Determination of amino acids and catecholamines derivatized with 3-(4-chlorobenzoyl)-2-quinolinecarboxaldehyde in PC12 and HEK293 cells by capillary electrophoresis with laser-induced fluorescence detection.
abstract: An effective micellar electrokinetic capillary chromatography with laser-induced fluorescence detection (MEKC-LIF) method has been proposed for the separation and the determination of 16 amino acids and two catecholamines using a new fluorogenic reagent, 3-(4-chlorobenzoyl)-2-quinolinecarboxaldehyde (Cl-BQCA), as the derivatizing reagent. The highest derivatization efficiency was achieved in pH 8.0 borate buffer at 50 °C for 50 min. The optimal separation of Cl-BQCA-labeled amines was obtained with a running buffer (pH 9.15) containing 120 mM boric acid, 38.5 mM sodium dodecyl sulfate, and 17% acetonitrile. The detection limit (S/N = 3) was found to be as low as 1.4 nM. The present method has been successfully used to detect amino acids and catecholamines in HEK293 and PC12 cell samples. This study explores the potential of MEKC-LIF with Cl-BQCA labeling as a tool for monitoring amino acids and catecholamines during the complex physiological and behavioral processes in various matrices.
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan, China.
authors.forename: Niu
authors.initials: N
authors.lastname: Zhang
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Guo
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.forename: Hua-Shan
authors.initials: HS
authors.lastname: Zhang
mesh_terms: D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D001577:Benzophenones / Q000737:chemistry; D002395:Catecholamines / Q000032:analysis*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000191:economics / Q000379:methods*; D005453:Fluorescence; D057809:HEK293 Cells; D006801:Humans; D057230:Limit of Detection; D016716:PC12 Cells; D011804:Quinolines / Q000737:chemistry; D051381:Rats; D015203:Reproducibility of Results; D012997:Solvents
chemical_list: C532738:3-(4-chlorobenzoyl)quinoline-2-carboxaldehyde; D000596:Amino Acids; D001577:Benzophenones; D002395:Catecholamines; D011804:Quinolines; D012997:Solvents