title: Identification of GPCR localization in detergent resistant membranes.
abstract: Lipid domains of the plasma membrane were originally described as a cell matrix insoluble in cold -nonionic detergents and enriched in glycosphingolipids. Because of these biochemical properties, these membrane domains were termed detergent-resistant membranes (DRMs) or detergent-insoluble -glycolipid-enriched (DIG) membranes. Membrane rafts and caveolae are two types of lipid domains that share these properties, as well as structural/functional dependence on membrane cholesterol. Membrane rafts and caveolae are believed to act as signaling platforms for ligand-activated receptors, thereby contributing to the regulation of receptor function. Here we describe a simple method to assess the association of GPCRs with detergent resistant membranes in native brain tissue and cultured cells.
authors: Dominick P. Purpura Department of Neuroscience, Albert Einstein College of Medicine, New York, NY, USA.
authors: Ranju
authors: R
authors: Kumari
authors: Anna
authors: A
authors: Francesconi