title: Microsatellite primers in red bayberry, Myrica rubra (Myricaceae).
abstract: OBJECTIVE
  Microsatellite markers were developed in Myrica rubra to investigate potential hybridization events within or between M. rubra and its closely related species.
  
  
  RESULTS
  Using an ISSR-suppression PCR method, 12 primer pairs were temporarily developed with GSG(GT)(6) as the primer for enriching microsatellite sequences and the genomic DNA of M. rubra cv. 'Heijing' as template. The average allele number per locus was 4.9 within 26 individuals including two species, M. rubra and M. nana. Three pairs of primers produced species-specific alleles, and the other nine showed polymorphisms among 26 accessions.
  
  
  CONCLUSIONS
  Results indicated that the ISSR-suppression PCR method is suitable for developing microsatellite markers, especially for this species with little understanding of genomic information. The developed microsatellite markers provide a useful tool for further studies of population structure within or between M. rubra and M. nana or other closely related species.
authors: Institute of Horticulture, Zhejiang Academy of Agricultural Sciences (ZAAS), Hangzhou 310021, PR China. xiexiaobo@yahoo.com
authors: Xiaobo
authors: X
authors: Xie
authors: Yingying
authors: Y
authors: Qiu
authors: Liping
authors: L
authors: Ke
authors: Xiliang
authors: X
authors: Zheng
authors: Guanting
authors: G
authors: Wu
authors: Jinqing
authors: J
authors: Chen
authors: Xingjiang
authors: X
authors: Qi
authors: Sangnag
authors: S
authors: Ahn