title: Influence of protein deposition on bacterial adhesion to contact lenses.
abstract: OBJECTIVE
  The aim of the study is to determine the adhesion of Gram positive and Gram negative bacteria onto conventional hydrogel (CH) and silicone hydrogel (SH) contact lens materials with and without lysozyme, lactoferrin, and albumin coating.
  
  
  METHODS
  Four lens types (three SH-balafilcon A, lotrafilcon B, and senofilcon A; one CH-etafilcon A) were coated with lysozyme, lactoferrin, or albumin (uncoated lenses acted as controls) and then incubated in Staphylococcus aureus (Saur 31) or either of two strains of Pseudomonas aeruginosa (Paer 6294 and 6206) for 24 h at 37 °C. The total counts of the adhered bacteria were determined using the H-thymidine method and viable counts by counting the number of colony-forming units on agar media.
  
  
  RESULTS
  All three strains adhered significantly lower to uncoated etafilcon A lenses compared with uncoated SH lenses (p < 0.05). Lysozyme coating on all four lens types increased binding (total and viable counts) of Saur 31 (p < 0.05). However, lysozyme coating did not influence P. aeruginosa adhesion (p > 0.05). Lactoferrin coating on lenses increased binding (total and viable counts) of Saur 31 (p < 0.05). Lactoferrin-coated lenses showed significantly higher total counts (p < 0.05) but significantly lower viable counts (p < 0.05) of adhered P. aeruginosa strains. There was a significant difference between the total and viable counts (p < 0.05) that were bound to lactoferrin-coated lenses. Albumin coating of lenses increased binding (total and viable counts) of all three strains (p < 0.05).
  
  
  CONCLUSIONS
  Lysozyme deposited on contact lenses does not possess antibacterial activity against certain bacterial strains, whereas lactoferrin possess an antibacterial effect against strains of P. aeruginosa.
authors: Department of Chemical Engineering, McMaster University, 1280 Main Street West, Hamilton, Ontario L8S4L7, Canada. slaksh@mcmaster.ca
authors: Lakshman N
authors: LN
authors: Subbaraman
authors: Roya
authors: R
authors: Borazjani
authors: Hua
authors: H
authors: Zhu
authors: Zhenjun
authors: Z
authors: Zhao
authors: Lyndon
authors: L
authors: Jones
authors: Mark D P
authors: MD
authors: Willcox