title: Fully human monoclonal antibody directed to proteolytic cleavage site in severe acute respiratory syndrome (SARS) coronavirus S protein neutralizes the virus in a rhesus macaque SARS model.
abstract: BACKGROUND
  There is still no effective method to prevent or treat severe acute respiratory syndrome (SARS), which is caused by SARS coronavirus (CoV). In the present study, we evaluated the efficacy of a fully human monoclonal antibody capable of neutralizing SARS-CoV in vitro in a Rhesus macaque model of SARS.
  
  
  METHODS
  The antibody 5H10 was obtained by vaccination of KM mice bearing human immunoglobulin genes with Escherichia coli-producing recombinant peptide containing the dominant epitope of the viral spike protein found in convalescent serum samples from patients with SARS.
  
  
  RESULTS
  5H10, which recognized the same epitope that is also a cleavage site critical for the entry of SARS-CoV into host cells, inhibited propagation of the virus and pathological changes found in Rhesus macaques infected with the virus through the nasal route. In addition, we analyzed the mode of action of 5H10, and the results suggested that 5H10 inhibited fusion between the virus envelope and host cell membrane. 5H10 has potential for use in prevention and treatment of SARS if it reemerges.
  
  
  CONCLUSIONS
  This study represents a platform to produce fully human antibodies against emerging infectious diseases in a timely and safe manner.
authors: Department of Infectious Diseases, National Center for Global Health and Medicine, 1-21-1 Toyama, Shinjuku-ku, Tokyo 162-8655, Japan. takiyam@ri.ncgm.go.jp
authors: Tohru
authors: T
authors: Miyoshi-Akiyama
authors: Isao
authors: I
authors: Ishida
authors: Masaya
authors: M
authors: Fukushi
authors: Keina
authors: K
authors: Yamaguchi
authors: Yusuke
authors: Y
authors: Matsuoka
authors: Takashi
authors: T
authors: Ishihara
authors: Masayoshi
authors: M
authors: Tsukahara
authors: Seisuke
authors: S
authors: Hatakeyama
authors: Norikazu
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authors: Itoh
authors: Aki
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authors: Morisawa
authors: Yoshiyuki
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authors: Yoshinaka
authors: Naoki
authors: N
authors: Yamamoto
authors: Zhang
authors: Z
authors: Lianfeng
authors: Qin
authors: Q
authors: Chuan
authors: Teruo
authors: T
authors: Kirikae
authors: Takehiko
authors: T
authors: Sasazuki
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