title: Transformation of geniposide into genipin by immobilized β-glucosidase in a two-phase aqueous-organic system.
abstract: Genipin is the bioactive compound of geniposide and a natural cross-linking agent. In order to improve the preparation process of genipin, the hydrolysis of geniposide to genipin by immobilized β-glucosidase in an aqueous-organic two-phase system was studied. β-glucosidase was immobilized by the crosslinking-embedding method using sodium alginate as the carrier. The optimum reaction temperature, pH value and time were 55 °C, 4.5 and 2.5 h, respectively. To reduce genipin hydrolysis and byproduct production the reaction was carried out in an aqueous-organic two-phase system comprising ethyl acetate and sodium acetate buffer. The product was analyzed by HPLC, UV, IR, and NMR. The yield of genipin was 47.81% and its purity was over 98% (HPLC).
authors: Experiment Center of Teaching & Learning, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors: Yi-Shun
authors: YS
authors: Yang
authors: Tong
authors: T
authors: Zhang
authors: Shai-Cheng
authors: SC
authors: Yu
authors: Yue
authors: Y
authors: Ding
authors: Li-Ying
authors: LY
authors: Zhang
authors: Chen
authors: C
authors: Qiu
authors: Duo
authors: D
authors: Jin
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