title: Analysis of trehalose-6-phosphate synthase (TPS) gene family suggests the formation of TPS complexes in rice.
abstract: Trehalose-6-phosphate (T6P), an intermediate in the trehalose biosynthesis pathway, is emerging as an important regulator of plant metabolism and development. T6P levels are potentially modulated by a group of trehalose-6-phosphate synthase (TPS) and trehalose-6-phosphate phosphatase (TPP) homologues. In this study, we have isolated 11 TPS genes encoding proteins with both TPS and TPP domains, from rice. Functional complement assays performed in yeast tps1 and tps2 mutants, revealed that only OsTPS1 encodes an active TPS enzyme and no OsTPS protein possesses TPP activity. By using a yeast two-hybrid analysis, a complicated interaction network occurred among OsTPS proteins, and the TPS domain might be essential for this interaction to occur. The interaction between OsTPS1 and OsTPS8 in vivo was confirmed by bimolecular fluorescence complementation and coimmunoprecipitation assays. Furthermore, our gel filtration assay showed that there may exist two forms of OsTPS1 (OsTPS1a and OsTPS1b) with different elution profiles in rice. OsTPS1b was particularly cofractionated with OsTPS5 and OsTPS8 in the 360 kDa complex, while OsTPS1a was predominantly incorporated into the complexes larger than 360 kDa. Collectively, these results suggest that OsTPS family members may form trehalose-6-phosphate synthase complexes and therefore potentially modify T6P levels to regulate plant development.
authors: Peking-Yale Joint Center for Plant Molecular Genetics and Agrobiotechnology, National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing, 100871, People's Republic of China.
authors: Baisheng
authors: B
authors: Zang
authors: Haowen
authors: H
authors: Li
authors: Wenjun
authors: W
authors: Li
authors: Xing Wang
authors: XW
authors: Deng
authors: Xiping
authors: X
authors: Wang
references: Biochem J. 2006 Jul 1;397(1):139-48
references: 16551270
references: Plant J. 1998 Mar;13(5):673-83
references: 9681009
references: Plant Physiol. 2008 Jan;146(1):97-107
references: 17981987
references: Proc Natl Acad Sci U S A. 2005 Aug 2;102(31):11118-23
references: 16046541
references: Plant J. 2002 Jan;29(2):225-35
references: 11851922
references: Plant Mol Biol. 2007 Mar;63(4):441-63
references: 17115300
references: Plant Physiol. 2000 Sep;124(1):105-14
references: 10982426
references: Mol Plant. 2010 Mar;3(2):406-19
references: 20100798
references: Plant Physiol. 2004 Aug;135(4):2330-47
references: 15310832
references: Mol Microbiol. 1997 May;24(4):687-95
references: 9194697
references: Planta. 2008 Jun;228(1):191-201
references: 18365248
references: Plant J. 2006 Jul;47(2):211-23
references: 16771775
references: Trends Plant Sci. 2001 Nov;6(11):510-3
references: 11701378
references: Plant Physiol. 2003 Feb;131(2):516-24
references: 12586876
references: Plant Physiol. 2004 Jun;135(2):969-77
references: 15181208
references: BMC Evol Biol. 2006 Dec 19;6:109
references: 17178000
references: J Plant Physiol. 2005 Feb;162(2):215-23
references: 15779831
references: Nature. 2007 Aug 23;448(7156):938-42
references: 17671505
references: Planta. 1997 Mar;201(3):293-7
references: 19343407
references: FEBS Lett. 1987 Aug 10;220(1):113-5
references: 3301407
references: J Biol Chem. 1998 Dec 11;273(50):33311-9
references: 9837904
references: Plant Physiol. 1997 Sep;115(1):159-169
references: 12223797
references: Protein Sci. 1998 Jul;7(7):1647-52
references: 9684901
references: Chem Biol. 2002 Dec;9(12):1337-46
references: 12498887
references: Proc Natl Acad Sci U S A. 2003 May 27;100(11):6849-54
references: 12748379
references: Plant J. 2006 Apr;46(1):69-84
references: 16553896
references: Biochem Biophys Res Commun. 2004 Jan 9;313(2):314-9
references: 14684162
references: Nucleic Acids Res. 2007 Jan;35(Database issue):D883-7
references: 17145706
references: Adv Carbohydr Chem Biochem. 1974;30:227-56
references: 4377836
references: Plant Physiol. 2004 Nov;136(3):3649-59
references: 15516499
references: Proc Natl Acad Sci U S A. 2002 Dec 10;99(25):15898-903
references: 12456878
references: Trends Plant Sci. 1999 Aug;4(8):315-319
references: 10431221
references: Plant J. 2004 Nov;40(3):428-38
references: 15469500
references: Glycobiology. 2003 Apr;13(4):17R-27R
references: 12626396
references: Plant Biotechnol J. 2004 Jan;2(1):71-82
references: 17166144
references: Nature. 2006 May 11;441(7090):227-30
references: 16688177
references: Plant J. 2007 Feb;49(3):463-91
references: 17217462
references: FEBS J. 2007 Mar;274(5):1192-201
references: 17257172
references: J Gen Microbiol. 1991 Feb;137(2):323-30
references: 1849964
references: Plant J. 1994 Aug;6(2):271-82
references: 7920717
references: J Ind Microbiol Biotechnol. 1997 Jan;18(1):30-6
references: 9079286
references: Plant Physiol. 1997 Jan;113(1):181-90
references: 9008394
references: Biotechniques. 1992 Jul;13(1):18-20
references: 1503765
references: Plant Physiol. 2009 Apr;149(4):1860-71
references: 19193861
references: Plant Cell. 2001 Nov;13(11):2393-407
references: 11701877
references: Plant Cell. 2004 Aug;16(8):2128-50
references: 15273295
references: Plant Physiol. 2004 Jun;135(2):879-90
references: 15181209
references: J Exp Bot. 2001 Sep;52(362):1817-26
references: 11520870
references: Plant Mol Biol. 2005 Aug;58(6):751-762
references: 16240171
references: Trends Plant Sci. 2002 May;7(5):193-5
references: 11992820
references: Mol Cell Proteomics. 2005 Oct;4(10):1614-25
references: 16030010
references: Plant Physiol. 1999 Apr;119(4):1473-82
references: 10198107
references: Yeast. 1992 Mar;8(3):183-92
references: 1315471
references: Eur J Biochem. 1993 Mar 1;212(2):315-23
references: 8444170
references: Eur J Biochem. 1992 Nov 1;209(3):951-9
references: 1425702
references: Planta. 2007 Nov;226(6):1411-21
references: 17628825
references: Plant Methods. 2006 Jun 29;2:13
references: 16808845
references: Nucleic Acids Res. 2004 Jan 1;32(Database issue):D377-82
references: 14681438
references: Plant Cell Environ. 2009 Aug;32(8):1015-32
references: 19344332
references: Microbiology (Reading). 1998 Mar;144 ( Pt 3):671-680
references: 9534237
references: Nucleic Acids Res. 1997 Dec 15;25(24):4876-82
references: 9396791
references: Proc Natl Acad Sci U S A. 1998 May 26;95(11):5857-64
references: 9600884
references: Biochimie. 2006 Oct;88(10):1505-10
references: 16828951
references: Plant J. 1998 Mar;13(5):685-9
references: 9681010
references: Gene. 1995 May 26;158(1):113-7
references: 7789793
references: Plant Mol Biol. 2007 Jul;64(4):371-86
references: 17453154