title: [Effect of rapamycin combined with cisplatin on head and neck squamous cancer cells regulated by CCL19].
abstract: OBJECTIVE
  To investigate the synergistic effects of rapamycin and cisplatin on head and neck squamous cancer cells regulated by chemokine (C-C motif) ligand 19 (CCL19).
  
  
  METHODS
  The role of rapamycin and cisplatin was detected on cell-cycle and apoptosis in CCL19 induced PCI-4B and PCI-37B cells by methyl thiazolyl tetrazolium (MTT) and flow cytometry (FCM). Dose-effect relationship parameters and combination index (CI) were calculated on the median-effect equation and multiple drug effect equation using computer software CalcuSyn. Statistical analysis was performed by the unpaired student's t-test.
  
  
  RESULTS
  Rapamycin and cisplatin could respectively increase the growth arrest, the proportion of G(1) phase and apoptosis of CCL19 induced cancer cells (P < 0.05). Under inhibitory concentration 50% (IC(50)), CI was less than 1, and in IC(75), it was more than 1 in PCI-4B cells. In PCI-37B cells, under IC(75), CI was less than 1, and in IC(90), it was more than 1.
  
  
  CONCLUSIONS
  Rapamycin and cisplatin can inhibit CCL19-regulated PCI-4B and PCI-37B cells' survival. The two drugs have synergistic effects when used in combination.
authors: Department of Oral and Maxillofacial Surgery, School of Stomatology, China Medical University, Shenyang 110002, China.
authors: Fa-yu
authors: FY
authors: Liu
authors: Zhen-jin
authors: ZJ
authors: Zhao
authors: Peng
authors: P
authors: Li
authors: Xue
authors: X
authors: Ding
authors: Chang-fu
authors: CF
authors: Sun