title: Streptococcus pneumoniae is desiccation tolerant and infectious upon rehydration.
abstract: Streptococcus pneumoniae (pneumococcus) is a frequent colonizer of the nasopharynx and one of the leading causative agents of otitis media, pneumonia, and meningitis. The current literature asserts that S. pneumoniae is transmitted person to person via respiratory droplets; however, environmental surfaces (fomites) have been linked to the spread of other respiratory pathogens. Desiccation tolerance has been to shown to be essential for long-term survival on dry surfaces. This study investigated the survival and infectivity of S. pneumoniae following desiccation under ambient conditions. We recovered viable bacteria after all desiccation periods tested, ranging from 1 h to 4 weeks. Experiments conducted under nutrient limitation indicate that desiccation is a condition separate from starvation. Desiccation of an acapsular mutant and 15 different clinical isolates shows that S. pneumoniae desiccation tolerance is independent of the polysaccharide capsule and is a species-wide phenomenon, respectively. Experiments demonstrating that nondesiccated and desiccated S. pneumoniae strains colonize the nasopharynx at comparable levels, combined with their ability to survive long-term desiccation, suggest that fomites may serve as alternate sources of pneumococcal infection.
  
  
  OBJECTIVE
  Even with the advent of multivalent capsular polysaccharide conjugate vaccines, S. pneumoniae continues to be a major cause of morbidity and mortality worldwide. Every year, there are approximately 7 million cases of pneumococcus-based otitis media in the United States alone, while pneumococcal invasive diseases are responsible for more than 1 million deaths globally. It is believed that the human upper respiratory tract is the sole niche of S. pneumoniae and, thus, that spread occurs via close contact with an infected individual. In this study, we characterized the desiccation tolerance of S. pneumoniae and found that it can survive for many weeks postdehydration and retain infectivity. Our results suggest that desiccation tolerance is an inherent trait of this genetically variable species and that fomites may be a source of transmission.
authors: Graduate Program in Molecular Microbiology, Sackler School of Graduate Biomedical Science, Howard Hughes Medical Institute and Department of Molecular Biology and Microbiology, School of Medicine, Tufts University, Boston, Massachusetts, USA.
authors: Rebecca L
authors: RL
authors: Walsh
authors: Andrew
authors: A
authors: Camilli
references: Theory Biosci. 2007 Aug;126(1):43-5
references: 18087756
references: Int J Food Microbiol. 2010 Mar 31;138(1-2):71-7
references: 20100628
references: J Clin Microbiol. 2007 Apr;45(4):1225-33
references: 17267625
references: Mol Microbiol. 2002 Sep;45(5):1389-406
references: 12207705
references: J Clin Microbiol. 1998 Jul;36(7):1938-41
references: 9650940
references: Lancet. 2009 Oct 31;374(9700):1543-56
references: 19880020
references: Microbiol Rev. 1994 Dec;58(4):755-805
references: 7854254
references: Infect Immun. 1987 May;55(5):1184-9
references: 3552992
references: Appl Environ Microbiol. 2000 Apr;66(4):1489-92
references: 10742231
references: J Infect Dis. 1998 Feb;177(2):368-77
references: 9466523
references: J Mol Med (Berl). 2010 Feb;88(2):97-102
references: 19898768
references: Clin Infect Dis. 2008 Mar 1;46(5):752-60
references: 18220477
references: Lancet Infect Dis. 2004 Mar;4(3):144-54
references: 14998500
references: Nat Rev Microbiol. 2008 Apr;6(4):288-301
references: 18340341
references: Res Microbiol. 2002 Jan-Feb;153(1):7-12
references: 11881900
references: BMC Genomics. 2009 Dec 30;10:642
references: 20042094
references: Microbiol Rev. 1995 Dec;59(4):591-603
references: 8531887
references: ISME J. 2008 Apr;2(4):393-403
references: 18273068
references: PLoS Med. 2010 Oct 05;7(10):
references: 20957191
references: J Bacteriol. 1998 Apr;180(7):1750-8
references: 9537371
references: Proc Natl Acad Sci U S A. 2010 Aug 10;107(32):14373-7
references: 20660760
references: Nat Rev Microbiol. 2008 Nov;6(11):827-37
references: 18923410
references: J Infect Dis. 2007 Nov 1;196(9):1346-54
references: 17922399
references: Am J Infect Control. 2007 Jun;35(5):342-6
references: 17577483
references: J Clin Microbiol. 2000 Feb;38(2):724-6
references: 10655374
references: J Appl Bacteriol. 1990 Mar;68(3):271-8
references: 2111304
references: Mol Microbiol. 2003 Nov;50(4):1103-10
references: 14622402
references: Infect Immun. 2007 Jan;75(1):83-90
references: 17088346
references: Appl Microbiol Biotechnol. 2006 Dec;73(3):703-12
references: 17053902
references: Proc R Soc Lond B Biol Sci. 1959 Mar 17;150(939):149-91
references: 13633975
references: Biol Rev Camb Philos Soc. 2004 Nov;79(4):849-69
references: 15682873
references: Mol Gen Genet. 1974;128(4):283-90
references: 4150368
references: Pediatr Infect Dis J. 2007 Jun;26(6):468-72
references: 17529860
references: BMC Infect Dis. 2006 Aug 16;6:130
references: 16914034
references: J Infect Dev Ctries. 2009 Sep 15;3(8):628-32
references: 19801807
references: Adv Space Res. 1992;12(4):239-47
references: 11538144
references: J Infect Dis. 1946 Jul-Aug;79:33-44
references: 20996926
references: Cornea. 1995 Sep;14(5):523-6
references: 8536467
references: Trends Microbiol. 2001 Nov;9(11):553-9
references: 11825716
references: Eur Respir J. 2005 Apr;25(4):693-700
references: 15802345
references: Infect Immun. 2001 Apr;69(4):2477-86
references: 11254610
references: J Bacteriol. 2007 Jan;189(1):38-51
references: 17041037
references: PLoS Pathog. 2007 Oct 26;3(10):1372-3
references: 17967057
references: BMC Vet Res. 2010 Nov 02;6:48
references: 21044298
references: J Med Microbiol. 1993 Jan;38(1):61-3
references: 8418296
references: Adv Biochem Eng Biotechnol. 1994;51:71-89
references: 8165952
references: Science. 2011 Jan 28;331(6016):430-4
references: 21273480
references: Lancet Infect Dis. 2008 Dec;8(12):785-95
references: 19022193