title: Characterization of acute renal allograft rejection by proteomic analysis of renal tissue in rat.
abstract: Rapid and reliable biomarkers of renal allograft rejection have not been available. This study aimed to investigate biomarkers in renal allograft tissue using proteomic analysis. Orthotopic kidney transplantations were performed using Fisher (F344) or Lewis rats as donors and Lewis rats as recipients. Syngenic control group (Group I) constituted F344-to-F344 orthotopic kidney allo-transplantations (n = 8); and allogenic group (Group II) consisted of F344-to-Lewis orthotopic kidney allo-transplantations (n = 8). Renal tissues were harvested 7 days after transplantation. Samples were analyzed using 2-D electrophoresis and matrix assisted laser desorption ionization-time of flight mass spectrometry. 6 differentially expressed proteins were identified between allogenic group and syngenic control group. A rat model of acute renal allograft rejection was successfully set up. Differentially expressed proteins in renal allograft tissue of rat were detected using proteomic analysis and might serve as novel diagnostic and therapeutic targets in human. Quantitative proteomics, using MALDL-TOF-MS methodology has the potential to provide a profiling and a deeper understanding of acute renal rejection.
authors: Department of Urology, The First Affiliated Hospital, Chongqing Medical University, 1 Youyi Road, Chongqing 400016, China. chengang@hotmail.com
authors: Gang
authors: G
authors: Chen
authors: Jing-Bin
authors: JB
authors: Huang
authors: Jie
authors: J
authors: Mi
authors: Yun-Feng
authors: YF
authors: He
authors: Xiao-Hou
authors: XH
authors: Wu
authors: Chun-Li
authors: CL
authors: Luo
authors: Si-Min
authors: SM
authors: Liang
authors: Jia-Bing
authors: JB
authors: Li
authors: Ya-Xiong
authors: YX
authors: Tang
authors: Jie
authors: J
authors: Li
references: J Am Soc Nephrol. 2005 Jun;16(6):1542-8
references: 15888558
references: Mol Biol Rep. 2009 May;36(5):909-15
references: 18454324
references: J Am Soc Nephrol. 2004 Dec;15(12):3240-8
references: 15579528
references: Trends Immunol. 2008 Sep;29(9):436-43
references: 18675588
references: Am J Transplant. 2008 Apr;8(4):753-60
references: 18294345
references: Annu Rev Pathol. 2008;3:189-220
references: 18039144
references: J Mol Biol. 2009 Feb 20;386(2):476-85
references: 19124027
references: Mol Biol Rep. 2009 Nov;36(8):2093-8
references: 19107576
references: Mol Biol Rep. 2002 Sep;29(3):265-80
references: 12463419
references: Transplantation. 2008 Jul 27;86(2):192-9
references: 18645476
references: Cell Cycle. 2006 Feb;5(3):266-70
references: 16410727
references: Saudi J Kidney Dis Transpl. 2005 Oct-Dec;16(4):520-39
references: 18202507
references: Nat Med. 2002 Jun;8(6):582-7
references: 12042808
references: Mol Biol Rep. 2010 Mar;37(3):1619-25
references: 19444643
references: Mol Biol Rep. 2010 Feb;37(2):711-6
references: 19437132
references: Nature. 2002 Dec 12;420(6916):629-35
references: 12478284
references: J Proteome Res. 2009 Jul;8(7):3430-8
references: 19485423
references: Curr Opin Organ Transplant. 2009 Feb;14(1):10-5
references: 19337140
references: Mol Biol Rep. 2009 Nov;36(8):2119-29
references: 19109764
references: J Proteome Res. 2005 Jul-Aug;4(4):1192-9
references: 16083269
references: Mol Biol Rep. 2009 Feb;36(2):263-72
references: 17987401
references: J Am Soc Nephrol. 2004 Jan;15(1):219-27
references: 14694176
references: Mol Biol Rep. 2009 Nov;36(8):2387-92
references: 19296238
references: Transplant Proc. 1989 Feb;21(1 Pt 2):1880-1
references: 2652611
references: Transplantation. 2009 Oct 15;88(7):942-51
references: 19935467
references: Am J Transplant. 2005 Apr;5(4 Pt 1):729-38
references: 15760396
references: Ther Drug Monit. 2006 Feb;28(1):19-22
references: 16418688
references: Ann Surg. 2003 May;237(5):660-4; discussion 664-5
references: 12724632
references: Transplantation. 2009 May 15;87(9):1330-9
references: 19424033
references: Proc Natl Acad Sci U S A. 2005 Aug 23;102(34):12153-8
references: 16093309
references: Pediatr Transplant. 2005 Dec;9(6):700-11
references: 16269039
references: Nephrol Dial Transplant. 2005 Dec;20(12):2613-9
references: 16204271
references: Transplant Proc. 2004 Jan-Feb;36(1):29-34
references: 15013292
references: Mol Biol Rep. 2009 Apr;36(4):813-21
references: 18523866
references: N Engl J Med. 2001 Mar 29;344(13):947-54
references: 11274620
references: N Engl J Med. 2004 Dec 23;351(26):2715-29
references: 15616206
references: Mol Biol Rep. 2009 Jul;36(6):1621-6
references: 18792803
references: Transplantation. 2000 Oct 15;70(7):1098-100
references: 11045649
references: Proteomics Clin Appl. 2007 Sep;1(9):983-1003
references: 21136752
references: Purinergic Signal. 2009 Sep;5(3):369-83
references: 19404775