title: [Patterns of osteoprotegerin or nuclear factor kappa B ligand gene expression in the treatment of bone defect with bone marrow stem cell transplantation and low-frequency vibration].
abstract: OBJECTIVE
  To explore the effect of low frequency vibration (LFV)on the osteogenic differentiation regulating capability of bone marrow stromal cell (BMSC)and the expressions of OPG (osteoprotegerin) mRNA and RANKL (nuclear factor kappa B ligand) mRNA through living animal experiment.
  
  
  METHODS
  Both BMSC transplantation and low-frequency vibration were employed to treat bone defects. The groups were randomized into non-vibration and vibration of different frequencies. The vibration group received vibrating interventions at Day 7 for 5 weeks. After vibrations, the BMSC OPG and RANKL mRNA of different frequency groups were detected.
  
  
  RESULTS
  The BMSC OPG and RANKL gene expressions significantly increased (P < 0.05), especially at 25 Hz (P < 0.01). And for the vibration group at 100 Hz, the BMSC OPG and RANKL gene expressions decreased (P < 0.05).
  
  
  CONCLUSIONS
  Low-frequency vibration may promote the osteogenic differentiation capability of BMSC probably through regulating the OPG/RANKL mRNA expression, directly promoting bone formation and inhibiting bone resorption.
authors: Department of Orthopedics, Second Affiliated Hospital, Dalian Medical University, Dalian 116031, China.
authors: Chuan-xiu
authors: CX
authors: Sun
authors: Wen-zhi
authors: WZ
authors: Zhao
authors: Sheng-wei
authors: SW
authors: He
authors: Xu
authors: X
authors: Fang
authors: Li-dong
authors: LD
authors: Mi
authors: Guang-yu
authors: GY
authors: DU
authors: Lu
authors: L
authors: Zhang
authors: Xue-gang
authors: XG
authors: Sun