title: Ultrastructure of Felis catus whole fetus (Fcwf-4) cell culture following infection with feline coronavirus.
abstract: Feline coronavirus (FCoV) consists of two biotypes based on their growth in cell culture and their antigenicity. Infections with FCoV are highly prevalent in the cat population worldwide. In this study, Felis catus whole fetus (Fcwf-4)cell culture was infected with FCoV UPM11C/08. Virus multiplication in cell culture was monitored and examined under the transmission electron microscope. The virus particles revealed the characteristic morphology of feline FCoV represented by envelope viruses surrounded by peplomers. Virus attachment and entry into the cell occurred 15 h post-infection (pi), and the myriad of virus particles were observed both extracellularly and intracellularly after 48 h pi. Thereafter, intracellular virus particles were observed to be present in vacuoles or present freely in the cytoplasm.
authors: Department of Veterinary Pathology and Microbiology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
authors: Amer
authors: A
authors: Alazawy
authors: Siti-Suri
authors: SS
authors: Arshad
authors: Mohd-Hair
authors: MH
authors: Bejo
authors: Abdul-Rahman
authors: AR
authors: Omar
authors: Tengku-Azmi
authors: TA
authors: Tengku Ibrahim
authors: Saeed
authors: S
authors: Sharif
authors: Faruku
authors: F
authors: Bande
authors: Kamarudin
authors: K
authors: Awang-Isa
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