title: Early detection of peritoneal dissemination of gastrointestinal cancers by reverse-transcriptase polymerase chain reaction.
abstract: We examined the usefulness of the reverse-transcriptase polymerase chain reaction (RT-PCR) analysis for E-cadherin mRNA in the early diagnosis of peritoneal dissemination of gastric and colorectal cancers. The RT-PCR analysis was performed on RNA samples extracted from cells which were collected preoperatively from ascites or intraperitoneally infused fluid. E-cadherin mRNA was detected in 2/2 cases (100%) graded into P-1 peritoneal dissemination, indicating the presence of metastatic cells. In the cases with P-2 and P-3 dissemination the gene expression was detected in 2/3 (67%) and 3/5 (60%), respectively. The RT-PCR analysis was more sensitive for P-1 dissemination than cytological examination. These results suggest that determination of E-cadherin gene expression is useful for the early detection of peritoneal dissemination in gastrointestinal cancer because of its high sensitivity and specificity.
authors: KANAZAWA UNIV,CANC RES INST,KANAZAWA,ISHIKAWA 920,JAPAN.
authors: T
authors: T
authors: Fujimura
authors: Y
authors: Y
authors: Yonemura
authors: I
authors: I
authors: Ninomiya
authors: K
authors: K
authors: Miwa
authors: I
authors: I
authors: Miyazaki
authors: Y
authors: Y
authors: Endo
authors: N
authors: N
authors: Ishikawa
authors: T
authors: T
authors: Obata
authors: M
authors: M
authors: Tanaka
authors: T
authors: T
authors: Sasaki