title: Regulation of the Na+/K+ ATPase by Klotho.
abstract: Klotho-hypomorphic (Klotho(hm)) mice suffer from renal salt wasting and hypovolemia despite hyperaldosteronism. The present study explored the effect of Klotho on renal Na(+)/K(+) ATPase activity. According to immunohistochemistry and confocal microscopy Na(+)/K(+) ATPase protein abundance in isolated collecting ducts was lower in Klotho(hm) mice than in their wild type littermates (Klotho(+/+)). Analysis with dual electrode voltage clamp recording showed that expression of Klotho in Xenopus oocytes increased the Na(+)/K(+) ATPase pump current. Treatment of Xenopus oocytes with Klotho protein similarly increased the pump current. In conclusion, Klotho increases the membrane abundance and activity of the Na(+)/K(+) ATPase. Decreased Na(+)/K(+) ATPase activity could thus contribute to the volume-depletion of klotho(hm) mice.
authors: Department of Physiology, University of Tübingen, Tübingen, Germany.
authors: Mentor
authors: M
authors: Sopjani
authors: Ioana
authors: I
authors: Alesutan
authors: Miribane
authors: M
authors: Dërmaku-Sopjani
authors: Shuchen
authors: S
authors: Gu
authors: Christine
authors: C
authors: Zelenak
authors: Carlos
authors: C
authors: Munoz
authors: Ana
authors: A
authors: Velic
authors: Michael
authors: M
authors: Föller
authors: Kevin P
authors: KP
authors: Rosenblatt
authors: Makoto
authors: M
authors: Kuro-o
authors: Florian
authors: F
authors: Lang