title: Cryptosporidium parvum oocyst viability and behaviour of the residual body during the excystation process.
abstract: This study was conducted as a comparative evaluation of time-dependent changes in the viability of purified Cryptosporidium parvum oocysts by means of different excystation methods. Oocyst samples were 2 weeks to 12 months old and were treated with bile or sodium taurocholate, partly after pretreatment with hypochlorite. Pretreatment markedly enhanced the excystation of younger oocyst samples but did not increase excystation rates of 9 or 12-month-old oocysts. A cell culture-PCR assay was used as a second indicator for oocyst viability and was most consistent with excystation trials including oocyst pretreatment. In experiments aiming at the determination of the behaviour of the oocyst residual body during excystation, it could be demonstrated that it might be involved in this process.
authors: Department of Biology, Namik Kemal University, 59030 Tekirdag, Turkey. sirrikar@yahoo.com
authors: Sirri
authors: S
authors: Kar
authors: Arwid
authors: A
authors: Daugschies
authors: Ayse
authors: A
authors: Cakmak
authors: Nadim
authors: N
authors: Yilmazer
authors: Katja
authors: K
authors: Dittmar
authors: Berit
authors: B
authors: Bangoura
references: Trends Parasitol. 2005 Mar;21(3):133-42
references: 15734661
references: J Parasitol. 1999 Oct;85(5):839-49
references: 10577718
references: Parasitol Res. 2010 Feb;106(3):683-7
references: 20107835
references: J Protozool. 1985 Nov;32(4):708-11
references: 4067883
references: Vet Parasitol. 2003 Jan 20;111(1):47-57
references: 12523978
references: J Protozool. 1987 Nov;34(4):398-402
references: 3123647
references: Water Res. 2004 Feb;38(4):818-62
references: 14769405
references: Mol Biochem Parasitol. 1998 Sep 1;95(1):21-31
references: 9763286
references: Infect Immun. 1982 Nov;38(2):471-5
references: 7141705
references: Appl Environ Microbiol. 1992 Nov;58(11):3488-93
references: 1482174
references: Parasitology. 2004 Mar;128(Pt 3):269-82
references: 15074876
references: Parasitology. 2011 Apr;138(5):573-7
references: 21232175
references: J Eukaryot Microbiol. 1999 Sep-Oct;46(5):56S-57S
references: 10519247
references: Vet Parasitol. 2011 May 11;177(3-4):366-70
references: 21242035
references: J Microbiol Methods. 2000 Dec 15;43(2):97-106
references: 11121608
references: FEMS Microbiol Lett. 2000 Feb 15;183(2):331-6
references: 10675606
references: Microsc Microanal. 2004 Oct;10(5):586-601
references: 15525433