title: Simultaneous assessment of autophagy and apoptosis using multispectral imaging cytometry.
abstract: Multiple stress pathways result in the induction of autophagy and apoptosis. Current methods (e.g., protein gel blot, microscopy) do not offer quantitative single-cell resolution, thus making it difficult to discern if these pathways are mutually exclusive or, in some situations, cooperative in executing cell death. We report a novel method that enables high-throughput, high-content assessment of LC3 puncta and caspase-3 cleavage at the single cell level.
authors: Centre d'Immunologie Humaine, Departement d'Immunologie, Institut Pasteur, Paris, France.
authors: Claire
authors: C
authors: de la Calle
authors: Pierre-Emmanuel
authors: PE
authors: Joubert
authors: Helen K W
authors: HK
authors: Law
authors: Milena
authors: M
authors: Hasan
authors: Matthew L
authors: ML
authors: Albert