title: Genetic association between GHSR1a 5'UTR-microsatellite and nt-7(C>A) loci and growth and carcass traits in Japanese Black cattle.
abstract: We carried out a genetic association study between five nucleotide polymorphisms (5'UTR microsatellite ((TG)(n)), nt-7(C>A), L24V, DelR242 and Intron 1 microsatellite) of the GHSR1a gene and growth and carcass traits in 1285 steers sired by 117 Japanese Black bulls in a progeny testing program. We report herein, a significant association between the 5'UTR microsatellite and nt-7(C>A) loci and growth and carcass traits. We also propose a translational hypothesis that the association is due to differences in the secondary structure of GHSR1b mRNA (the non-spliced type with the 5'UTR microsatellite) among the GHSR1a gene haplotypes. Furthermore, we predicted the potential increase in profitability due to increased carcass weight in cow-calf fattening enterprises through planned matings based on DNA testing of the 5'UTR microsatellite. Statistical analysis revealed that the 5'UTR microsatellite locus had a significant additive effect on carcass weight (CW) and average daily gain (ADG), but not on beef marbling score (BMS). One of the four major microsatellite alleles (19-TG allele) with an allele frequency of 0.145, had a significantly (P < 0.0007) desirable effect on CW and ADG. We concluded that the 19-TG allele could potentially be economically useful nucleotide markers for growth and carcass traits in Japanese Black cattle.
authors: National Institute of Livestock and Grassland Science, Tsukuba, Japan. mkomatsu@affrc.go.jp
authors: Masanori
authors: M
authors: Komatsu
authors: Tomohito
authors: T
authors: Itoh
authors: Yuki
authors: Y
authors: Fujimori
authors: Masahiro
authors: M
authors: Satoh
authors: Yoshiyuki
authors: Y
authors: Miyazaki
authors: Hideaki
authors: H
authors: Takahashi
authors: Kazuhiro
authors: K
authors: Shimizu
authors: Aduli E O
authors: AE
authors: Malau-Aduli
authors: Mitsuo
authors: M
authors: Morita