title: High spatial resolution label-free detection of antigen-antibody binding on patterned surface by imaging ellipsometry.
abstract: High spatial resolution and large area thickness mapping of label-free protein microarray has been achieved using imaging ellipsometry (IE) under optimized conditions. The protein patterns with feature size down to 8×8 μm(2) was readily imaged, and the binding between the surface immobilized antigen and the antibody was monitored. Quantitative thickness analysis of antibody-antigen binding on the 32×32 μm(2) micron spots was successfully performed, and we have obtained a limit of detection as low as 1.2 pg/spot. This work demonstrates that appropriately optimized IE could be used as a highly sensitive and high through-put label-free technique for studying surface antigen-antibody recognition in sub-40 μm scale.
authors: State Key Laboratory of Applied Organic Chemistry (SKLAOC), College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, Gansu 730000, China.
authors: Meng-Jie
authors: MJ
authors: Chang
authors: Chao-Ran
authors: CR
authors: Pang
authors: Jun
authors: J
authors: Liu
authors: Hua
authors: H
authors: Bai
authors: Jun
authors: J
authors: Deng
authors: Zhu-Guo
authors: ZG
authors: Xu
authors: Hao-Li
authors: HL
authors: Zhang