title: Relationship between microlymphatic vessel density within tumors and lymph node metastasis.
abstract: We assessed the relationship between microlymphatic vessel density (MLD) within tumors and lymph node metastasis. The SCID mice were injected subcutaneously with various human tumor cells. DNA was extracted from lymph nodes for specific detection of a human beta-globin-related sequence. The discrimination of lymphatic vessels was done by enzyme-histochemistry for 5'-nucleotidase in endothelial cells after an adequate inhibition of the activity. The nodal metastasis could be detected in mice with PC-14, and they developed high MLD. Mice without metastasis had low MLD excepting OST cells. Experimentally, we have observed high MLD within PC-14 tumors and its tendency toward lymph node metastasis.
authors: KANAZAWA UNIV,DEPT EXPT THERAPEUT,SCH MED,CANC RES INST,KANAZAWA,ISHIKAWA 920,JAPAN.
authors: Y
authors: Y
authors: Ohta
authors: M
authors: M
authors: Tanaka
authors: Y
authors: Y
authors: Endo
authors: S
authors: S
authors: Murakami
authors: M
authors: M
authors: Oda
authors: T
authors: T
authors: Sasaki
authors: Y
authors: Y
authors: Watanabe