title: Identification of a microRNA that activates gene expression by repressing nonsense-mediated RNA decay.
abstract: Nonsense-mediated decay (NMD) degrades both normal and aberrant transcripts harboring stop codons in particular contexts. Mutations that perturb NMD cause neurological disorders in humans, suggesting that NMD has roles in the brain. Here, we identify a brain-specific microRNA-miR-128-that represses NMD and thereby controls batteries of transcripts in neural cells. miR-128 represses NMD by targeting the RNA helicase UPF1 and the exon-junction complex core component MLN51. The ability of miR-128 to regulate NMD is a conserved response occurring in frogs, chickens, and mammals. miR-128 levels are dramatically increased in differentiating neuronal cells and during brain development, leading to repressed NMD and upregulation of mRNAs normally targeted for decay by NMD; overrepresented are those encoding proteins controlling neuron development and function. Together, these results suggest the existence of a conserved RNA circuit linking the microRNA and NMD pathways that induces cell type-specific transcripts during development.
authors: Department of Biochemistry and Molecular Biology, University of Texas M.D. Anderson Cancer Center, Houston, TX 77033, USA.
authors: Ivone G
authors: IG
authors: Bruno
authors: Rachid
authors: R
authors: Karam
authors: Lulu
authors: L
authors: Huang
authors: Anjana
authors: A
authors: Bhardwaj
authors: Chih H
authors: CH
authors: Lou
authors: Eleen Y
authors: EY
authors: Shum
authors: Hye-Won
authors: HW
authors: Song
authors: Mark A
authors: MA
authors: Corbett
authors: Wesley D
authors: WD
authors: Gifford
authors: Jozef
authors: J
authors: Gecz
authors: Samuel L
authors: SL
authors: Pfaff
authors: Miles F
authors: MF
authors: Wilkinson
references: Genome Biol. 2004;5(3):R13
references: 15003116
references: J Biochem. 2010 Jul;148(1):13-24
references: 20495010
references: Dev Dyn. 2010 Jul;239(7):1977-87
references: 20549732
references: Mol Psychiatry. 2010 Jul;15(7):767-76
references: 19238151
references: Hum Mol Genet. 2001 Jan 15;10(2):99-105
references: 11152657
references: J Cell Sci. 2010 Oct 1;123(Pt 19):3303-15
references: 20826458
references: Annu Rev Biochem. 2007;76:51-74
references: 17352659
references: Cell. 2009 Jan 23;136(2):215-33
references: 19167326
references: Nat Genet. 2004 Oct;36(10):1073-8
references: 15448691
references: Cancer Res. 2008 Nov 15;68(22):9125-30
references: 19010882
references: Neuroreport. 2007 Feb 12;18(3):297-300
references: 17314675
references: Comp Biochem Physiol B Biochem Mol Biol. 2008 Jul;150(3):301-11
references: 18495509
references: EMBO J. 2007 Apr 4;26(7):1820-30
references: 17363904
references: Proc Natl Acad Sci U S A. 2010 Jul 6;107(27):12186-91
references: 20566848
references: Curr Opin Cell Biol. 2009 Jun;21(3):394-402
references: 19359157
references: Nat Genet. 2007 Sep;39(9):1127-33
references: 17704778
references: Nature. 2008 Oct 16;455(7215):912-8
references: 18923513
references: Genes Dev. 2008 May 15;22(10):1381-96
references: 18483223
references: Genome Biol. 2007;8(8):R173
references: 17711588
references: J Mol Med (Berl). 2009 Jan;87(1):43-51
references: 18810376
references: Nat Neurosci. 2010 May;13(5):551-8
references: 20364144
references: Mol Psychiatry. 2011 Mar;16(3):238-9
references: 20479756
references: Cell. 2007 Jul 13;130(1):179-91
references: 17632064
references: Nucleic Acids Res. 2006 Jan 1;34(Database issue):D135-9
references: 16381831
references: PLoS One. 2008;3(11):e3652
references: 18987751
references: Cell. 2010 Oct 29;143(3):379-89
references: 21029861
references: Science. 2007 Aug 31;317(5842):1220-4
references: 17761882
references: Neurosci Lett. 2011 Apr 8;493(1-2):18-23
references: 21315137
references: Neurogenetics. 2008 Jul;9(3):153-61
references: 18563458
references: Genes Dev. 2007 Mar 1;21(5):531-6
references: 17344415
references: Exp Neurol. 2011 Jan;227(1):172-9
references: 21035445
references: Biochem J. 2010 Sep 15;430(3):365-77
references: 20795950
references: Nat Struct Mol Biol. 2009 Jul;16(7):747-53
references: 19503078
references: Trends Biochem Sci. 2006 Nov;31(11):639-46
references: 17010613
references: Nucleic Acids Res. 2011 May;39(9):3686-94
references: 21227923
references: J Biol Chem. 2004 Jun 25;279(26):27039-49
references: 15096518
references: Proc Natl Acad Sci U S A. 2011 Jan 11;108(2):816-21
references: 21187403
references: Dev Dyn. 2006 Jul;235(7):1895-907
references: 16680723
references: Mol Cell Biol. 2009 Jul;29(13):3517-28
references: 19414594
references: Eur J Neurosci. 2005 Mar;21(6):1469-77
references: 15845075
references: RNA. 2008 Mar;14(3):432-44
references: 18230762
references: J Biol Chem. 2003 Mar 21;278(12):10465-76
references: 12533540