title: Immunophenotypic and gene expression analysis of monoclonal B-cell lymphocytosis shows biologic characteristics associated with good prognosis CLL.
abstract: Monoclonal B-cell lymphocytosis (MBL) is a hematologic condition wherein small B-cell clones can be detected in the blood of asymptomatic individuals. Most MBL have an immunophenotype similar to chronic lymphocytic leukemia (CLL), and 'CLL-like' MBL is a precursor to CLL. We used flow cytometry to identify MBL from unaffected members of CLL kindreds. We identified 101 MBL cases from 622 study subjects; of these, 82 individuals with MBL were further characterized. In all, 91 unique MBL clones were detected: 73 CLL-like MBL (CD5(+)CD20(dim)sIg(dim)), 11 atypical MBL (CD5(+)CD20(+)sIg(+)) and 7 CD5(neg) MBL (CD5(neg)CD20(+)sIg(neg)). Extended immunophenotypic characterization of these MBL subtypes was performed, and significant differences in cell surface expression of CD23, CD49d, CD79b and FMC-7 were observed among the groups. Markers of risk in CLL such as CD38, ZAP70 and CD49d were infrequently expressed in CLL-like MBL, but were expressed in the majority of atypical MBL. Interphase cytogenetics was performed in 35 MBL cases, and del 13q14 was most common (22/30 CLL-like MBL cases). Gene expression analysis using oligonucleotide arrays was performed on seven CLL-like MBL, and showed activation of B-cell receptor associated pathways. Our findings underscore the diversity of MBL subtypes and further clarify the relationship between MBL and other lymphoproliferative disorders.
authors: Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA. mark.lanasa@duke.edu
authors: M C
authors: MC
authors: Lanasa
authors: S D
authors: SD
authors: Allgood
authors: S L
authors: SL
authors: Slager
authors: S S
authors: SS
authors: Dave
authors: C
authors: C
authors: Love
authors: G E
authors: GE
authors: Marti
authors: N E
authors: NE
authors: Kay
authors: C A
authors: CA
authors: Hanson
authors: K G
authors: KG
authors: Rabe
authors: S J
authors: SJ
authors: Achenbach
authors: L R
authors: LR
authors: Goldin
authors: N J
authors: NJ
authors: Camp
authors: B K
authors: BK
authors: Goodman
authors: C M
authors: CM
authors: Vachon
authors: L G
authors: LG
authors: Spector
authors: L Z
authors: LZ
authors: Rassenti
authors: J F
authors: JF
authors: Leis
authors: J P
authors: JP
authors: Gockerman
authors: S S
authors: SS
authors: Strom
authors: T G
authors: TG
authors: Call
authors: M
authors: M
authors: Glenn
authors: J R
authors: JR
authors: Cerhan
authors: M C
authors: MC
authors: Levesque
authors: J B
authors: JB
authors: Weinberg
authors: N E
authors: NE
authors: Caporaso
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