title: M6 membrane protein plays an essential role in Drosophila oogenesis.
abstract: We had previously shown that the transmembrane glycoprotein M6a, a member of the proteolipid protein (PLP) family, regulates neurite/filopodium outgrowth, hence, M6a might be involved in neuronal remodeling and differentiation. In this work we focused on M6, the only PLP family member present in Drosophila, and ortholog to M6a. Unexpectedly, we found that decreased expression of M6 leads to female sterility. M6 is expressed in the membrane of the follicular epithelium in ovarioles throughout oogenesis. Phenotypes triggered by M6 downregulation in hypomorphic mutants included egg collapse and egg permeability, thus suggesting M6 involvement in eggshell biosynthesis. In addition, RNAi-mediated M6 knockdown targeted specifically to follicle cells induced an arrest of egg chamber development, revealing that M6 is essential in oogenesis. Interestingly, M6-associated phenotypes evidenced abnormal changes of the follicle cell shape and disrupted follicular epithelium in mid- and late-stage egg chambers. Therefore, we propose that M6 plays a role in follicular epithelium maintenance involving membrane cell remodeling during oogenesis in Drosophila.
authors: Instituto de Investigaciones Biotecnológicas, Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad Nacional de General San Martín, Buenos Aires, Argentina. mpzappia@iib.unsam.edu.ar
authors: María Paula
authors: MP
authors: Zappia
authors: Marcela Adriana
authors: MA
authors: Brocco
authors: Silvia C
authors: SC
authors: Billi
authors: Alberto C
authors: AC
authors: Frasch
authors: María Fernanda
authors: MF
authors: Ceriani
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