title: [Correlation of breast tumor pathogenesis and somatic mutations on the basis of mitochondrial DNA whole genome].
abstract: OBJECTIVE
  To investigate the correlation of somatic mutations in whole genome of mitochondrial DNA (mtDNA) in patients with breast tumor.
  
  
  METHODS
  The DNA of tumor tissue and peripheral blood in 4 benign breast tumor patients from August 2009 to December 2009 in our hospital were extracted. The mtDNA whole genomes were amplified by polymerase chain reaction (PCR) and the mutations of products screened by sequencing to compare the difference of mutation distribution between tumor tissue and peripheral blood. The likelihood of somatic mutations in tumor tissue was determined.
  
  
  RESULTS
  The mutation spectrum of mtDNA genomes was obtained by PCR and sequencing. Then a phylogenetic tree was constructed to identify their haplogroups (D4i, G1, R9b, N9a). Their private mutations in peripheral blood were detected and the somatic mutation at 16292 position was found in one patient (haplogroups: R9b).
  
  
  CONCLUSIONS
  Based on the extensively study on the mtDNA genomes from the tumor issues of 4 patients, our current report observed only a single somatic variation from the control region, no any further mutations with potential function from the coding region were observed.
authors: Department of Breast, First Peoples' Hospital of Kunming, Affiliated Ganmei Hospital, Kunming Medical University, Kunming 650011, China.
authors: Yue
authors: Y
authors: Yang
authors: Jun-dong
authors: JD
authors: He
authors: Xue-jun
authors: XJ
authors: Guo
authors: Yan-hua
authors: YH
authors: Che
authors: Yong
authors: Y
authors: Zhang
authors: Li
authors: L
authors: Li