title: Quantitative determination of daumone in rat plasma by liquid chromatography-mass spectrometry.
abstract: Daumone, 6-(3,5-dihydroxy-6-methyl-tetrahydro-pyran-2-yloxy)-heptanoic acid is a pheromone secreted by Caenorhabditis elegans, and has been known as a pivotal regulator of chemosensory processes in development and ageing. A quantification method using mass spectrometry was developed for the determination of daumone in rat plasma. After simple protein precipitation with acetonitrile including an internal standard, the analytes were chromatographed on a reversed-phase column and detected by liquid chromatography/tandem mass spectrometry with electrospray ionization. The accuracy and precision of the assay were in accordance with FDA regulations for validation of bioanalytical methods. This method was applied to measure the plasma daumone concentrations after a single intravenous administration of daumone in rats.
authors: College of Pharmacy, Yeungnam University, Kyoungsan, Kyoungbuk 712-749, South Korea.
authors: Keumhan
authors: K
authors: Noh
authors: Jung Hyun
authors: JH
authors: Park
authors: Jong Hee
authors: JH
authors: Park
authors: Minkyu
authors: M
authors: Kim
authors: Mankil
authors: M
authors: Jung
authors: Hunjoo
authors: H
authors: Ha
authors: Kwang-Il
authors: KI
authors: Kwon
authors: Hwa Jeong
authors: HJ
authors: Lee
authors: Wonku
authors: W
authors: Kang