title: Acute restraint stress in zebrafish: behavioral parameters and purinergic signaling.
abstract: Despite the extensive knowledge about the effects of acute restraint stress (ARS) in rodents, zebrafish research is still elementary in this field, and the consequences of stress on purinergic system are unclear. Therefore, we evaluated the effects of ARS on behavior, biochemical, and molecular parameters in zebrafish brain. Animals were submitted to a 90 min ARS protocol and tested for anxiety levels, exploratory behavior, and memory performance. Furthermore, we analyzed ectonucleotidase and adenosine deaminase activities and their gene expression profile, as well as transcription of adenosine receptors. ARS increased anxiety, but did not impair locomotion or cognition. ARS significantly increased ATP hydrolysis, decreased cytosolic ADA activity, and changed the entpd and adora gene expression. In conclusion, ARS disturbed zebrafish behavior, and we hypothesize that the augmentation in adenosine-mediated signaling may be a strategy to reestablish homeostasis and normal behavior after a stressful event.
authors: Laboratório de Neuroquímica e Psicofarmacologia, Departamento de Biologia Celular e Molecular, Faculdade de Biociências, Pontifícia Universidade Católica do Rio Grande do Sul, Avenida Ipiranga, 6681 Prédio 12D, sala 340, Porto Alegre, RS 90619-900, Brazil. angelopiato@gmail.com
authors: Angelo L
authors: AL
authors: Piato
authors: Denis B
authors: DB
authors: Rosemberg
authors: Katiucia M
authors: KM
authors: Capiotti
authors: Anna M
authors: AM
authors: Siebel
authors: Ana P
authors: AP
authors: Herrmann
authors: Gabriele
authors: G
authors: Ghisleni
authors: Monica R
authors: MR
authors: Vianna
authors: Maurício R
authors: MR
authors: Bogo
authors: Diogo R
authors: DR
authors: Lara
authors: Carla D
authors: CD
authors: Bonan
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