title: Comprehensive identification of glycated peptides and their glycation motifs in plasma and erythrocytes of control and diabetic subjects.
abstract: Nonenzymatic glycation of proteins sets the stage for formation of advanced glycation end-products and development of chronic complications of diabetes. In this report, we extended our previous methods on proteomics analysis of glycated proteins to comprehensively identify glycated proteins in control and diabetic human plasma and erythrocytes. Using immunodepletion, enrichment, and fractionation strategies, we identified 7749 unique glycated peptides, corresponding to 3742 unique glycated proteins. Semiquantitative comparisons showed that glycation levels of a number of proteins were significantly increased in diabetes and that erythrocyte proteins were more extensively glycated than plasma proteins. A glycation motif analysis revealed that some amino acids were favored more than others in the protein primary structures in the vicinity of the glycation sites in both sample types. The glycated peptides and corresponding proteins reported here provide a foundation for potential identification of novel markers for diabetes, hyperglycemia, and diabetic complications in future studies.
authors: Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington 99352, USA.
authors: Qibin
authors: Q
authors: Zhang
authors: Matthew E
authors: ME
authors: Monroe
authors: Athena A
authors: AA
authors: Schepmoes
authors: Therese R W
authors: TR
authors: Clauss
authors: Marina A
authors: MA
authors: Gritsenko
authors: Da
authors: D
authors: Meng
authors: Vladislav A
authors: VA
authors: Petyuk
authors: Richard D
authors: RD
authors: Smith
authors: Thomas O
authors: TO
authors: Metz
references: Diabetes Obes Metab. 2007 May;9(3):233-45
references: 17391149
references: Int J Pharm. 2006 Sep 28;322(1-2):136-45
references: 16920285
references: Biochem Biophys Res Commun. 2001 Nov 16;288(5):1108-11
references: 11700025
references: J Biol Chem. 1992 Jul 25;267(21):14987-97
references: 1321822
references: Diabetes. 2005 Nov;54(11):3103-11
references: 16249432
references: J Biol Chem. 2007 Mar 16;282(11):8510-20
references: 17229729
references: J Biol Chem. 1986 Oct 15;261(29):13542-5
references: 3759977
references: Anal Chem. 2008 Apr 1;80(7):2379-90
references: 18307322
references: Prog Clin Biol Res. 1989;304:43-67
references: 2675036
references: Atherosclerosis. 1996 Dec 20;127(2):155-65
references: 9125305
references: Diabetes Care. 2006 Jun;29(6):1405-7
references: 16732034
references: J Biol Chem. 1979 May 25;254(10):3892-8
references: 438166
references: Adv Exp Med Biol. 1998;434:213-35
references: 9598202
references: J Pathol. 2010 May;221(1):13-25
references: 20186922
references: J Clin Endocrinol Metab. 2008 Apr;93(4):1143-52
references: 18182449
references: Eur J Pharm Biopharm. 2008 Sep;70(1):42-50
references: 18583113
references: Clin Chim Acta. 1987 Jul 15;166(2-3):143-53
references: 3621595
references: Biochem J. 2003 Nov 1;375(Pt 3):581-92
references: 12885296
references: J Exp Med. 1991 Oct 1;174(4):931-9
references: 1655949
references: Anal Chem. 2007 Aug 1;79(15):5991-9
references: 17571855
references: Electrophoresis. 2004 May;25(9):1278-9
references: 15174049
references: Biochem Biophys Res Commun. 2003 Sep 12;309(1):196-200
references: 12943682
references: J Proteome Res. 2005 Jan-Feb;4(1):53-62
references: 15707357
references: Rapid Commun Mass Spectrom. 2008 Oct;22(19):3027-34
references: 18763275
references: Diabetes. 1991 Jun;40(6):701-8
references: 2040386
references: J Mass Spectrom. 2006 Nov;41(11):1459-69
references: 17063450
references: Cardiovasc Res. 2004 Sep 1;63(4):582-92
references: 15306213
references: Exp Clin Endocrinol Diabetes. 2011 Jun;119(6):366-9
references: 21104578
references: Glycobiology. 2006 Sep;16(9):844-53
references: 16762979
references: Exp Gerontol. 2004 Feb;39(2):249-54
references: 15036419
references: Mol Cell Proteomics. 2008 Oct;7(10):1963-73
references: 18632595
references: Biochem Biophys Res Commun. 1975 Nov 3;67(1):103-9
references: 1201013
references: Biochem Biophys Res Commun. 1968 Jul 11;32(1):86-93
references: 4874776
references: Anal Biochem. 2008 Feb 15;373(2):179-91
references: 18158144
references: Annu Rev Med. 1995;46:223-34
references: 7598459
references: Circ Res. 2003 Dec 12;93(12):1159-69
references: 14670831
references: Ann N Y Acad Sci. 2005 Jun;1043:9-19
references: 16037217
references: N Engl J Med. 1986 Feb 13;314(7):403-8
references: 3945267
references: Rapid Commun Mass Spectrom. 2007;21(5):661-6
references: 17279487
references: Tex Rep Biol Med. 1980-1981;40:373-85
references: 6798703
references: Mass Spectrom Rev. 2006 Sep-Oct;25(5):775-97
references: 16625652
references: Am J Pathol. 1998 Oct;153(4):1149-55
references: 9777946
references: Ann N Y Acad Sci. 2005 Jun;1043:1-8
references: 16037216
references: Diabetes Care. 2005 Mar;28(3):645-52
references: 15735202
references: JAMA. 1996 Oct 16;276(15):1246-52
references: 8849753
references: Nucleic Acids Res. 1990 Oct 25;18(20):6097-100
references: 2172928
references: J Biol Chem. 1985 Sep 5;260(19):10629-36
references: 4030761
references: Diabetes. 2006 Jul;55(7):1961-9
references: 16804064
references: Eur J Biochem. 1993 Aug 1;215(3):567-72
references: 8354263
references: Diabetes Metab Res Rev. 2006 Nov-Dec;22(6):444-54
references: 16506275
references: Diabetes Care. 2007 Oct;30(10):2756-8
references: 17901536
references: Mol Cell Proteomics. 2010 Mar;9(3):579-92
references: 19955080
references: Anal Chem. 2008 Dec 15;80(24):9822-9
references: 18989935
references: J Proteome Res. 2009 Feb;8(2):754-69
references: 19093874
references: Neurotox Res. 2002 May;4(3):191-209
references: 12829400
references: Clin Chem. 2008 Oct;54(10):1608-16
references: 18687737
references: J Proteome Res. 2008 May;7(5):2025-32
references: 18396901
references: Neurosci Lett. 2004 Nov 23;371(2-3):226-9
references: 15519762
references: Mass Spectrom Rev. 2006 May-Jun;25(3):450-82
references: 16429408
references: Diabetes. 2005 Oct;54(10):2952-60
references: 16186398
references: Mol Cell Proteomics. 2006 Nov;5(11):2167-74
references: 16854842
references: Anal Chem. 2007 Jan 15;79(2):477-85
references: 17222010
references: Biochim Biophys Acta. 2000 Dec 20;1498(2-3):99-111
references: 11108954
references: J Proteome Res. 2007 Jun;6(6):2323-30
references: 17488106
references: Exp Gerontol. 2001 Sep;36(9):1527-37
references: 11525875
references: Proc Natl Acad Sci U S A. 1998 Feb 3;95(3):1301-6
references: 9448326
references: J Diabetes Sci Technol. 2009 Jul 01;3(4):635-43
references: 20144305