title: Suction conditions for minimizing the production of free hemoglobin during blood salvage using an autotransfusion apparatus.
abstract: BACKGROUND
  Three kinds of conditions should be considered to reduce free hemoglobin production using an autologous cell salvage device. They are the negative suction pressure, the size of suction tip, and the air contact during suction. We want to examine which condition is the most important factor to produce free hemoglobin.
  
  
  METHODS
  One pack of red blood cell and one pack of fresh frozen plasma with the same blood type were mixed. They were aspirated based on the two suction pressure (-150 mmHg or -300 mmHg), three sizes of suction tips, and the two conditions of air contact, in which the suction tip was located in the surface of blood or in the middle of the blood. Seven ml sized EDTA tube was used to collect 5 ml blood. All the procedure repeated ten times. Free hemoglobin, total hemoglobin, and hematocrit were measured. Hemolysis ratio was calculated with following formula. Hemolysis ratio = (new free hemoglobin production) × (100-hematocrit) / (total hemoglobin).
  
  
  RESULTS
  Free hemoglobin production and hemolysis ratio were increased when the suction tip was positioned in the surface than when it was in the middle of the blood. The pressure of negative suction and three kinds of the suction tips did not influence the production of free hemoglobin nor the hemolysis ratio.
  
  
  CONCLUSIONS
  The air contact is the most important factor to reduce hemolysis using autologous cell salvage device. Suction pressure or suction tip diameter have little influence to produce hemolysis.
authors: Department of Anesthesiology and Pain Medicine, College of Medicine, Seoul National University, Seoul, Korea.
authors: Sang-Bum
authors: SB
authors: An
authors: Eun Su
authors: ES
authors: Choi
authors: Wonsik
authors: W
authors: Ahn
references: J Lab Clin Med. 1968 May;71(5):784-90
references: 5647682
references: Anesth Analg. 2007 Apr;104(4):869-75
references: 17377098
references: Clin Chem Lab Med. 2010 May;48(5):677-83
references: 20158444
references: Int Orthop. 2009 Dec;33(6):1615-8
references: 18853156
references: Transfus Apher Sci. 2010 Feb;42(1):33-7
references: 19932982
references: Anesth Analg. 2009 Jul;109(1):38-42
references: 19535693
references: Transfusion. 1996 Jan;36(1):57-60
references: 8607155
references: Antioxid Redox Signal. 2010 Feb;12(2):275-91
references: 19659434
references: Transfusion. 2009 Nov;49(11):2374-83
references: 19903293
references: Antioxid Redox Signal. 2010 Feb;12(2):261-73
references: 19659436