title: Vaccinia virus lacking A17 induces complex membrane structures composed of open membrane sheets.
abstract: The vaccinia virus (VACV) precursor membrane, the crescent, consists of an open membrane sheet and is formed by rupture of a cellular compartment. Here, we asked whether A17, a viral membrane protein, plays a role in membrane rupture. Without A17 synthesis, crescents are not formed, and instead, tubular and vesicular membranes accumulate (Rodriguez et al. in J Virol 69:4640-4648, 1). We used electron tomography (ET) to analyze whether the viral membranes lacking A17 consist of open membrane sheets. Tubular, vesicular and so far not described onion-shaped membranes, which consisted of open membrane sheets, were seen. Thus, the data show that membrane rupture occurs independently of the A17 protein.
authors: European Molecular Biology Laboratory, Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany. chlanda@embl.de
authors: Petr
authors: P
authors: Chlanda
authors: Maria Alejandra
authors: MA
authors: Carbajal
authors: Androniki
authors: A
authors: Kolovou
authors: Maho
authors: M
authors: Hamasaki
authors: Marek
authors: M
authors: Cyrklaff
authors: Gareth
authors: G
authors: Griffiths
authors: Jacomine
authors: J
authors: Krijnse-Locker