title: Singular features of trypanosomatids' phosphotransferases involved in cell energy management.
abstract: Trypanosomatids are responsible for economically important veterinary affections and severe human diseases. In Africa, Trypanosoma brucei causes sleeping sickness or African trypanosomiasis, while in America, Trypanosoma cruzi is the etiological agent of Chagas disease. These parasites have complex life cycles which involve a wide variety of environments with very different compositions, physicochemical properties, and availability of metabolites. As the environment changes there is a need to maintain the nucleoside homeostasis, requiring a quick and regulated response. Most of the enzymes required for energy management are phosphotransferases. These enzymes present a nitrogenous group or a phosphate as acceptors, and the most clear examples are arginine kinase, nucleoside diphosphate kinase, and adenylate kinase. Trypanosoma and Leishmania have the largest number of phosphotransferase isoforms ever found in a single cell; some of them are absent in mammals, suggesting that these enzymes are required in many cellular compartments associated to different biological processes. The presence of such number of phosphotransferases support the hypothesis of the existence of an intracellular enzymatic phosphotransfer network that communicates the spatially separated intracellular ATP consumption and production processes. All these unique features make phosphotransferases a promising start point for rational drug design for the treatment of human trypanosomiasis.
authors: Laboratorio de Biología Molecular de Trypanosoma cruzi (LBMTC), Instituto de Investigaciones Médicas "Alfredo Lanari", Universidad de Buenos Aires and CONICET, Combatientes de Malvinas 3150, 1427 Buenos Aires, Argentina.
authors: Claudio A
authors: CA
authors: Pereira
authors: León A
authors: LA
authors: Bouvier
authors: María de Los Milagros
authors: Mde L
authors: Cámara
authors: Mariana R
authors: MR
authors: Miranda
references: FEBS Lett. 2007 Jul 24;581(18):3507-11
references: 17624336
references: EMBO J. 2000 Dec 1;19(23):6371-81
references: 11101510
references: J Cell Biol. 1984 Apr;98(4):1178-84
references: 6715405
references: J Mol Biol. 1998 Sep 11;282(1):167-79
references: 9733648
references: J Cell Biochem. 1983;23(1-4):13-26
references: 6327737
references: Eur J Biochem. 1992 Oct 1;209(1):207-16
references: 1327770
references: FEMS Microbiol Lett. 2006 Aug;261(1):64-8
references: 16842360
references: FEBS Lett. 2003 Nov 6;554(1-2):201-5
references: 14596940
references: Proc Natl Acad Sci U S A. 1997 Aug 19;94(17):9000-5
references: 9256424
references: J Bioenerg Biomembr. 2000 Jun;32(3):247-58
references: 11768308
references: Exp Parasitol. 2008 Sep;120(1):103-7
references: 18534579
references: Biochem J. 1985 Apr 1;227(1):113-24
references: 3158309
references: J Eukaryot Microbiol. 2002 Jan-Feb;49(1):82-5
references: 11908902
references: Annu Rev Physiol. 2001;63:289-325
references: 11181958
references: EMBO J. 2005 Dec 7;24(23):4029-40
references: 16270030
references: Curr Genet. 1987;12(6):405-11
references: 2834097
references: Nature. 2006 Mar 9;440(7081):224-7
references: 16525475
references: J Eukaryot Microbiol. 1999 Nov-Dec;46(6):566-70
references: 10568030
references: Kinetoplastid Biol Dis. 2003 Jun 25;2(1):7
references: 12848897
references: Plant Cell Physiol. 2002 Nov;43(11):1276-84
references: 12461127
references: Mol Biochem Parasitol. 2008 Apr;158(2):163-75
references: 18242727
references: Parasitology. 2008 Dec;135(14):1661-6
references: 18980701
references: Mol Biochem Parasitol. 1992 Sep;54(2):185-99
references: 1435859
references: Trends Parasitol. 2009 Oct;25(10):482-90
references: 19748317
references: Biotechnol Lett. 2003 Jul;25(13):1013-7
references: 12889807
references: J Bioenerg Biomembr. 2003 Feb;35(1):31-40
references: 12848339
references: J Biol Chem. 2002 Jul 5;277(27):24427-34
references: 11967264
references: Mol Biol Evol. 2006 Apr;23(4):838-45
references: 16452116
references: Proc Natl Acad Sci U S A. 2009 Aug 4;106(31):12694-9
references: 19625628
references: J Cell Physiol. 2000 Feb;182(2):144-9
references: 10623877
references: EMBO J. 1993 Jun;12(6):2275-9
references: 8389692
references: J Biol Chem. 2004 Nov 26;279(48):50142-9
references: 15377659
references: Exp Parasitol. 2006 Dec;114(4):341-4
references: 16725140
references: J Bioenerg Biomembr. 2000 Jun;32(3):277-84
references: 11768311
references: Proteins. 2007 Oct 1;69(1):209-12
references: 17623863
references: Science. 2005 Jul 15;309(5733):409-15
references: 16020725
references: Int J Biol Macromol. 2009 Mar 1;44(2):149-55
references: 19100283
references: FEBS Lett. 2002 Aug 28;526(1-3):111-4
references: 12208515
references: J Biol Chem. 1993 May 25;268(15):11326-34
references: 8496185
references: Science. 1993 Jul 23;261(5120):478-80
references: 8392752
references: Mol Biochem Parasitol. 2004 Aug;136(2):211-20
references: 15478799
references: Biol Direct. 2006 Mar 23;1:8
references: 16556314
references: Int J Mol Sci. 2009 Apr 17;10(4):1729-72
references: 19468337
references: Cell. 1990 Nov 30;63(5):933-40
references: 2175255
references: FASEB J. 1992 Oct;6(13):3153-61
references: 1397837
references: Nature. 1989 Nov 9;342(6246):177-80
references: 2509941
references: Biochim Biophys Acta. 1998 Jun 10;1365(1-2):71-8
references: 9693724
references: J Biol Chem. 2005 Mar 25;280(12):11781-9
references: 15657034
references: J Mol Evol. 1989 Nov;29(5):381-95
references: 2533272
references: J Bioenerg Biomembr. 2000 Jun;32(3):237-46
references: 11768307
references: Biochem J. 1987 Jul 1;245(1):13-8
references: 2959272
references: Mol Gen Genet. 1972;116(3):199-210
references: 4558645
references: Biochim Biophys Acta. 1997 Dec 5;1343(2):152-9
references: 9434106
references: J Cell Sci. 2007 Feb 1;120(Pt 3):478-91
references: 17227795
references: J Exp Biol. 2003 Jun;206(Pt 12):2039-47
references: 12756286
references: J Biol Chem. 1999 Aug 6;274(32):22821-9
references: 10428867
references: Mol Biochem Parasitol. 1987 Nov;26(1-2):1-10
references: 3323902
references: Mol Cell Biochem. 1998 Jul;184(1-2):169-82
references: 9746320
references: BMC Microbiol. 2010 Jan 26;10:20
references: 20102621
references: Cancer Res. 1993 May 1;53(9):1971-3
references: 8481897
references: Acta Trop. 1982 Mar;39(1):61-72
references: 6122364
references: J Biol Chem. 2000 Nov 10;275(45):35570-6
references: 10952986
references: FEBS Lett. 1996 May 6;385(3):214-20
references: 8647254
references: J Biol Chem. 2003 Apr 11;278(15):12937-45
references: 12562761
references: J Insect Physiol. 2001 Jul;47(7):739-747
references: 11356421
references: Proc Natl Acad Sci U S A. 2001 Jun 19;98(13):7623-8
references: 11390963
references: FEBS Lett. 1977 Aug 15;80(2):360-4
references: 142663
references: PLoS Med. 2007 Dec;4(12):e332
references: 18162039
references: Biochem Soc Trans. 2005 Nov;33(Pt 5):967-71
references: 16246022
references: Antimicrob Agents Chemother. 2004 Jan;48(1):69-74
references: 14693520
references: Proc Natl Acad Sci U S A. 2008 Nov 18;105(46):17718-23
references: 19008351
references: FEBS Lett. 2001 Jun 1;498(1):22-5
references: 11389891
references: J Bioenerg Biomembr. 2000 Jun;32(3):215-25
references: 11768305
references: Science. 2005 Jul 15;309(5733):416-22
references: 16020726
references: Exp Parasitol. 1989 Aug;69(2):150-6
references: 2546792
references: J Biol Chem. 2000 Jan 14;275(2):1495-501
references: 10625703
references: J Biol Chem. 2003 Apr 11;278(15):12854-63
references: 12562769
references: J Biol Chem. 2002 Sep 6;277(36):32849-54
references: 12095995
references: Eur J Biochem. 1994 Jun 15;222(3):949-54
references: 8026505
references: J Biol Chem. 2003 Apr 11;278(15):13133-42
references: 12569107
references: Comp Biochem Physiol Part D Genomics Proteomics. 2006 Sep;1(3):300-8
references: 20483262
references: J Exp Biol. 2001 Mar;204(Pt 6):1063-71
references: 11222125
references: Biochim Biophys Acta. 2006 Jun;1760(6):913-21
references: 16567051
references: Cell Motil Cytoskeleton. 2006 May;63(5):245-53
references: 16572395
references: Mol Cell Biochem. 2004 Jan-Feb;256-257(1-2):13-27
references: 14977167
references: Annu Rev Biochem. 1985;54:831-62
references: 3896131
references: Arch Insect Biochem Physiol. 2004 Dec;57(4):166-77
references: 15540275
references: J Biol Chem. 2005 Apr 1;280(13):12451-60
references: 15647263
references: Mol Biol Cell. 2004 Jul;15(7):3257-65
references: 15146060
references: Mol Biochem Parasitol. 1995 Mar;70(1-2):119-29
references: 7637692
references: Comp Biochem Physiol B. 1986;85(2):285-8
references: 3780180
references: Mol Biochem Parasitol. 2006 Jun;147(2):193-206
references: 16546274
references: J Mol Biol. 1988 Aug 20;202(4):909-12
references: 2845103
references: Proc Natl Acad Sci U S A. 2002 Jul 23;99(15):10156-61
references: 12119406
references: J Biol Chem. 2004 Oct 29;279(44):45337-46
references: 15326192
references: Kinetoplastid Biol Dis. 2003 Oct 28;2(1):11
references: 14613499
references: Int J Parasitol. 2001 May 1;31(5-6):482-90
references: 11334933
references: Biochemistry. 2005 Dec 6;44(48):15774-86
references: 16313181
references: Gene. 2000 Oct 31;257(2):251-7
references: 11080591
references: J Cell Sci. 2002 Jul 1;115(Pt 13):2651-8
references: 12077356
references: Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Jul 1;66(Pt 7):862-5
references: 20606293
references: Cell Struct Funct. 2010;35(1):23-30
references: 20215702
references: Philos Trans R Soc Lond B Biol Sci. 2006 Jan 29;361(1465):101-18
references: 16553311
references: Cell Mol Biol Res. 1995;41(5):333-46
references: 8867780
references: Mol Biochem Parasitol. 1995 May;71(2):279-83
references: 7477113
references: J Biol Chem. 1991 Jun 15;266(17):10781-6
references: 2040598
references: Mol Cell Proteomics. 2009 Jul;8(7):1527-38
references: 19346560
references: Eur J Biochem. 2003 Nov;270(21):4339-47
references: 14622299
references: Biochem J. 1992 Jan 1;281 ( Pt 1):21-40
references: 1731757
references: J Biol Chem. 1996 Dec 20;271(51):32886-93
references: 8955129
references: Trends Parasitol. 2006 Oct;22(10):484-91
references: 16920028
references: J Biol Chem. 1992 Sep 5;267(25):18182-9
references: 1325460
references: J Biol Chem. 2002 Oct 11;277(41):38001-12
references: 12138089
references: Mol Biol Cell. 2004 Aug;15(8):3891-902
references: 15194815
references: Lancet. 2003 Nov 1;362(9394):1469-80
references: 14602444
references: J Biol Chem. 2002 Aug 30;277(35):31303-9
references: 12036963
references: Proc Natl Acad Sci U S A. 2008 Feb 12;105(6):1999-2004
references: 18245376
references: J Biol Chem. 2003 Dec 5;278(49):49625-35
references: 14506274
references: J Biol Chem. 1993 Dec 5;268(34):25780-9
references: 8245015
references: J Eukaryot Microbiol. 2003 Mar-Apr;50(2):132-4
references: 12744526
references: Eur J Biochem. 1997 Dec 1;250(2):326-31
references: 9428681