title: Screening of soy protein-derived hypotriglyceridemic di-peptides in vitro and in vivo.
abstract: BACKGROUND
  Soy protein and soy peptides have attracted considerable attention because of their potentially beneficial biological properties, including antihypertensive, anticarcinogenic, and hypolipidemic effects. Although soy protein isolate contains several bioactive peptides that have distinct physiological activities in lipid metabolism, it is not clear which peptide sequences are responsible for the triglyceride (TG)-lowering effects. In the present study, we investigated the effects of soy protein-derived peptides on lipid metabolism, especially TG metabolism, in HepG2 cells and obese Otsuka Long-Evans Tokushima fatty (OLETF) rats.
  
  
  RESULTS
  In the first experiment, we found that soy crude peptide (SCP)-LD3, which was prepared by hydrolyze of soy protein isolate with endo-type protease, showed hypolipidemic effects in HepG2 cells and OLETF rats. In the second experiment, we found that hydrophilic fraction, separated from SCP-LD3 with hydrophobic synthetic absorbent, revealed lipid-lowering effects in HepG2 cells and OLETF rats. In the third experiment, we found that Fraction-C (Frc-C) peptides, fractionated from hydrophilic peptides by gel permeation chromatography-high performance liquid chromatography, significantly reduced TG synthesis and apolipoprotein B (apoB) secretion in HepG2 cells. In the fourth experiment, we found that the fraction with 0.1% trifluoroacetic acid, isolated from Frc-C peptides by octadecylsilyl column chromatography, showed hypolipidemic effects in HepG2 cells. In the final experiment, we found that 3 di-peptides, Lys-Ala, Val-Lys, and Ser-Tyr, reduced TG synthesis, and Ser-Tyr additionally reduced apoB secretion in HepG2 cells.
  
  
  CONCLUSIONS
  Novel active peptides with TG-lowering effects from soy protein have been isolated.
authors: Department of Applied Biochemistry and Food Science, Saga University, Saga 840-8502, Japan.
authors: Nao
authors: N
authors: Inoue
authors: Koji
authors: K
authors: Nagao
authors: Kotaro
authors: K
authors: Sakata
authors: Naomi
authors: N
authors: Yamano
authors: Pathma Elgoda Ranawakage
authors: PE
authors: Gunawardena
authors: Seo-Young
authors: SY
authors: Han
authors: Toshiro
authors: T
authors: Matsui
authors: Toshihiro
authors: T
authors: Nakamori
authors: Hitoshi
authors: H
authors: Furuta
authors: Kiyoharu
authors: K
authors: Takamatsu
authors: Teruyoshi
authors: T
authors: Yanagita
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