title: The FbaB-type fibronectin-binding protein of Streptococcus pyogenes promotes specific invasion into endothelial cells.
abstract: Invasive serotype M3 Streptococcus pyogenes are among the most frequently isolated organisms from patients suffering from invasive streptococcal disease and have the potential to invade primary human endothelial cells (EC) via a rapid and efficient mechanism. FbaB protein, the fibronectin-binding protein expressed by M3 S. pyogenes, was herein identified as a potent invasin for EC. By combining heterologous gene expression with allelic replacement, we demonstrate that FbaB is essential and sufficient to trigger EC invasion via a Rac1-dependent phagocytosis-like uptake. FbaB-mediated uptake follows the classical endocytic pathway with lysosomal destination. FbaB is demonstrated to be a streptococcal invasin exhibiting EC tropism. FbaB thus initiates a process that may contribute to the deep tissue tropism and spread of invasive S. pyogenes isolates into the vascular EC lining.
authors: Department of Medical Microbiology, Helmholtz Centre for Infection Research, 38124 Braunschweig, Germany.
authors: Silva
authors: S
authors: Amelung
authors: Andreas
authors: A
authors: Nerlich
authors: Manfred
authors: M
authors: Rohde
authors: Barbara
authors: B
authors: Spellerberg
authors: Jason N
authors: JN
authors: Cole
authors: Victor
authors: V
authors: Nizet
authors: Gursharan S
authors: GS
authors: Chhatwal
authors: Susanne R
authors: SR
authors: Talay
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