title: Evaluation of Effect of Taxus baccata Leaves Extract on Bronchoconstriction and Bronchial Hyperreactivity in Experimental Animals.
abstract: The present investigation was undertaken to evaluate the bronchodilating effect and bronchial hyperreactivity of alcoholic extract of Taxus baccata Linn. (AET) leaves in experimental animals. Bronchodilator activity of AET was studied on the histamine and acetylcholine aerosol induced bronchospasm in guinea pigs and bronchial hyperreactivity was studied on bronchoalveolar lavage fluid (BALF) in the egg albumin sensitized guinea pigs and by histopathological studies. In vitro mast cell stabilizing activity was studied using compound 48/80 as a degranulating agent. Treatment with AET (200 and 400 mg/kg, p.o., for 7 days) showed significant protection against histamine and acetylcholine aerosol induced bronchospasm in guinea pigs. Significant decrease in the total leukocyte and differential leukocyte count in the BALF of the egg albumin sensitized guinea pigs was observed by administration of AET (200 and 400 mg/kg, p.o., for 15 days). AET dose dependently protected the mast cell disruption induced by compound 48/80. These results suggest that AET not only has bronchodilating activity but also decreases bronchial hyperreactivity by decreasing the infiltration of inflammatory cells in the airway and inhibiting the release of histamine like mediators from the mast cell by stabilizing it.
authors: Department of Pharmacology, C. K. Pithawalla Institute of Pharmaceutical Science and Research, Surat - 395 007, Gujarat, India.
authors: Pk
authors: P
authors: Patel
authors: Kv
authors: K
authors: Patel
authors: Tr
authors: T
authors: Gandhi
references: J Allergy Clin Immunol. 1993 Jul;92(1 Pt 2):148-54
references: 8335862
references: Clin Otolaryngol Allied Sci. 2003 Aug;28(4):291-303
references: 12871241
references: Am Rev Respir Dis. 1989 Jun;139(6):1383-9
references: 2543245
references: Int Arch Allergy Immunol. 1993;100(2):99-106
references: 7680258
references: J Ethnopharmacol. 1996 Nov;54(2-3):77-84
references: 8953421
references: Phytochemistry. 2003 Aug;63(7):789-93
references: 12877920
references: Phytochemistry. 1999 Apr;50(8):1267-304
references: 10326254
references: Bioorg Med Chem. 2003 May 1;11(9):1995-2000
references: 12670650
references: J Ethnopharmacol. 2003 Dec;89(2-3):265-70
references: 14611890
references: Appl Microbiol Biotechnol. 2001 Oct;57(1-2):13-9
references: 11693909
references: J Nat Prod. 1999 Oct;62(10):1448-72
references: 10543916
references: Am Rev Respir Dis. 1993 Sep;148(3):713-9
references: 8368644
references: Am Rev Respir Dis. 1987 Feb;135(2):433-40
references: 2433975
references: Am J Respir Cell Mol Biol. 1993 Jul;9(1):44-53
references: 8338676
references: J Immunol. 1991 Mar 15;146(6):1829-35
references: 1672334
references: Proc Natl Acad Sci U S A. 1988 Sep;85(18):6880-4
references: 2970644
references: Pharmacol Rev. 1998 Dec;50(4):515-96
references: 9860804
references: J Pharmacol Methods. 1990 Mar;23(1):13-9
references: 2304348
references: Biochem Pharmacol. 1999 Sep 1;58(5):759-65
references: 10449184
references: Z Naturforsch C J Biosci. 2003 Jan-Feb;58(1-2):65-9
references: 12622229
references: Indian J Exp Biol. 1995 Jan;33(1):38-40
references: 9135673
references: Blood. 1992 Jun 15;79(12):3101-9
references: 1596561
references: Immunol Today. 1992 Dec;13(12):501-7
references: 1361126
references: Am Rev Respir Dis. 1990 Dec;142(6 Pt 1):1407-13
references: 2252260
references: Am Rev Respir Dis. 1990 Aug;142(2):434-57
references: 2200318
references: Fitoterapia. 2001 Jan;72(1):59-61
references: 11163943
references: Am Rev Respir Dis. 1993 Oct;148(4 Pt 2):S1-26
references: 8214958
references: Biol Pharm Bull. 2001 Feb;24(2):167-71
references: 11217086