title: Intracisternal rSV40 administration provides effective pan-CNS transgene expression.
abstract: Potential genetic treatments for many generalized central nervous system (CNS) diseases require transgene expression throughout the CNS. Using oxidant stress and apoptosis caused by HIV-1 envelope gp120 as a model, we studied pan-CNS neuroprotective gene delivery into the cisterna magna (CM). Recombinant SV40 vectors carrying Cu/Zn superoxide dismutase or glutathione peroxidase were injected into rat CMs following intraperitoneal administration of mannitol. Sustained transgene expression was seen in neurons throughout the CNS. On challenge, 8 weeks later with gp120 injected into the caudate putamen, significant neuroprotection was documented. Thus, intracisternal administration of antioxidant-carrying rSV40 vectors may be useful in treating widespread CNS diseases such as HIV-1-associated neurocognitive disorders characterized by oxidative stress.
authors: Department of Pathology, Anatomy and Cell Biology, Thomas Jefferson University, Philadelphia, PA, USA. jplouboutin@hotmail.com
authors: J-P
authors: JP
authors: Louboutin
authors: B A S
authors: BA
authors: Reyes
authors: L
authors: L
authors: Agrawal
authors: E J
authors: EJ
authors: Van Bockstaele
authors: D S
authors: DS
authors: Strayer