title: Characterization of monoclonal antibodies against canine P-selectin glycoprotein ligand-1 (PSGL-1).
abstract: Thirteen different monoclonal antibodies against canine P-selectin glycoprotein ligand-1 (cPSGL-1) were obtained by immunization of rats with cells of a canine lymphoma cell line (Ema). O-sialoglycoprotein endopeptidase treatment of Ema cells showed that all of these antibodies recognized O-glycosylated peptides of canine PSGL-1. Experiments using deletion or point mutants of cPSGL-1 indicated that these antibodies could be categorized into several groups based on their cPSGL-1 recognition characteristics. These anti-cPSGL-1 monoclonal antibodies will be useful for analysis of the canine P-selectin and PSGL-1 system.
authors: Laboratory of Veterinary Internal Medicine, Department of Veterinary Medicine, Faculty of Agriculture, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan.
authors: Saori
authors: S
authors: Umeki
authors: Ryoichi
authors: R
authors: Suzuki
authors: Masayuki
authors: M
authors: Shimojima
authors: Yasuo
authors: Y
authors: Ema
authors: Takuma
authors: T
authors: Yanase
authors: Hiroyuki
authors: H
authors: Iwata
authors: Masaru
authors: M
authors: Okuda
authors: Takuya
authors: T
authors: Mizuno