title: [A novel recombinant human interferon alpha2b with high antivirus activity by combinatorial mutagenesis].
abstract: In order to create a novel recombinant human interferon alpha2b (rh-IFN alpha2b) with higher biological activity, we subjected the rational designed sequence of rh-IFN alpha2b to direct evolution by strategy of the combinatorial mutagenesis. The amino acid residues at multiple sites of 52-53-55, 103-107, and 121-125 were simultaneously mutated. The resulted gene of the mutated rh-IFN a2b was cloned into the pET28a and expressed in E. coli BL21 Condon plus (RIL). The anti-virus activity of the novel interferon alpha2b was 9.3 x 10(7) IU/mg, 93 times higher than the wild type (1 x 10(6) IU/mg). The results showed that the multiple point mutation used in this study could effectively combine the site effects of rh-IFN alpha2b and increase its biological activity.
authors: Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University, Tianjin 300072, China. grzhao@tju.edu.cn
authors: Guangrong
authors: G
authors: Zhao
authors: Lei
authors: L
authors: Du
authors: Linqi
authors: L
authors: Zhu