title: Decrease in antibody somatic hypermutation frequency under extreme, extended spaceflight conditions.
abstract: Somatic hypermutation diversifies antibody binding sites by introducing point mutations in the variable domains of rearranged immunoglobulin genes. In this study, we analyzed somatic hypermutation in variable heavy-chain (VH) domains of specific IgM antibodies of the urodele amphibian Pleurodeles waltl, immunized either on Earth or onboard the Mir space station. To detect somatic hypermutation, we aligned the variable domains of IgM heavy-chain transcripts with the corresponding VH gene. We also quantified NF-κB and activation-induced cytidine deaminase transcripts. Results were compared with those obtained using control animals immunized on Earth. Our data show that, as in most species of ectotherms, somatic hypermutation in P. waltl exhibits a mutational bias toward G and C bases. Furthermore, we show for the first time that somatic hypermutation occurs in space following immunization but at a lower frequency. This decrease is not due to a decrease in food intake or of the B-cell receptor/antigen interaction or to the absence of the germinal center-associated nuclear protein. It likely results from the combination of several spaceflight-associated changes, such as the severe reduction in T-cell activation, important perturbations of the cytoskeleton, and changes in the distribution of lymphocyte subpopulations and adhesion molecule expression.
authors: Faculty of Medicine, Development and Immunogenetics, Nancy-University, Vandœuvre-lès-Nancy, France.
authors: Matthieu
authors: M
authors: Bascove
authors: Nathan
authors: N
authors: Guéguinou
authors: Bérénice
authors: B
authors: Schaerlinger
authors: Guillemette
authors: G
authors: Gauquelin-Koch
authors: Jean-Pol
authors: JP
authors: Frippiat