title: Expression of interleukin-4 receptor α in human corneal epithelial cells.
abstract: OBJECTIVE
  We previously reported that human conjunctival epithelial cells expressed functioning interleukin-4 receptor α (IL-4Rα). In this study, we investigated whether human corneal epithelial cells also express functioning IL-4Rα.
  
  
  METHODS
  The presence of IL-4Rα mRNA and protein in human corneal epithelium was examined by reverse-transcriptase polymerase chain reaction (RT-PCR) and immunohistology, respectively. The cell surface expression of IL-4Rα and the transcripts upregulated upon IL-4Rα ligand (IL-4 or IL-13) stimulation were examined by flow cytometry and quantitative RT-PCR, respectively, using immortalized human corneal-limbal epithelial (HCLE) cells.
  
  
  RESULTS
  The mRNA and protein of IL-4Rα were detected in human corneal epithelium. Flow cytometry analysis showed the cell surface expression of IL-4Rα protein. Quantitative RT-PCR assay of HCLE cells showed the upregulation of the transcripts tumor necrosis factor alpha-induced protein 6 (TNFAIP6), RAS guanyl-releasing protein 1 (RASGRP1), carbonic anhydrase II (CA2), cytokine-inducible SH2-containing protein (CISH), hyaluronan synthase 3 (HAS3), calpain 14 (CAPN14), endothelin receptor type A (EDNRA), cathepsin C (CTSC), and lecithin retinol acyltransferase (LRAT) as well as human conjunctival epithelial cells.
  
  
  CONCLUSIONS
  Human corneal epithelial cells expressed functioning IL-4Rα, and stimulation of its ligands, IL-4 and IL-13, could induce the expression of various genes, e.g., antiinflammatory molecule genes such as TNFAIP6 and CISH and cellular differentiation and proliferation-related molecule genes such as RASGRP1, HAS3, EDNRA, and LRAT.
authors: Department of Ophthalmology, Kyoto Prefectural University of Medicine, 465 Kajii-cho, Hirokoji-agaru, Kawaramachi-dori, Kamigyo-ku, Kyoto, 602-0841, Japan. mueta@koto.kpu-m.ac.jp.|Research Center for Inflammation and Regenerative Medicine, Faculty of Life and Medical Sciences, Doshisha University, Kyoto, Japan. mueta@koto.kpu-m.ac.jp.
authors: Mayumi
authors: M
authors: Ueta
authors: Department of Ophthalmology, Kyoto Prefectural University of Medicine, 465 Kajii-cho, Hirokoji-agaru, Kawaramachi-dori, Kamigyo-ku, Kyoto, 602-0841, Japan.
authors: Chie
authors: C
authors: Sotozono
authors: Department of Ophthalmology, Kyoto Prefectural University of Medicine, 465 Kajii-cho, Hirokoji-agaru, Kawaramachi-dori, Kamigyo-ku, Kyoto, 602-0841, Japan.
authors: Shigeru
authors: S
authors: Kinoshita
references: J Immunol. 2004 Sep 1;173(5):3337-47
references: 15322197
references: Br J Ophthalmol. 2010 Nov;94(11):1528-32
references: 20657019
references: Invest Ophthalmol Vis Sci. 1994 Apr;35(5):2577-84
references: 8163345
references: J Allergy Clin Immunol. 2007 Dec;120(6):1457-9
references: 17900677
references: BMC Genomics. 2004 Mar 23;5(1):21
references: 15038835
references: Br J Ophthalmol. 2010 Sep;94(9):1239-43
references: 20610477
references: Eye Contact Lens. 2010 Sep;36(5):269-81
references: 20703156
references: Br J Ophthalmol. 2008 Mar;92(3):411-6
references: 18211936
references: Br J Ophthalmol. 2011 May;95(5):742-3
references: 20881026
references: Biochem Biophys Res Commun. 2005 May 27;331(1):285-94
references: 15845391
references: Biochim Biophys Acta. 2009 Mar;1791(3):220-8
references: 19171200
references: Invest Ophthalmol Vis Sci. 2008 May;49(5):1809-13
references: 18263811
references: Gene. 2001 Aug 22;274(1-2):245-52
references: 11675017
references: Am J Respir Cell Mol Biol. 2007 Jul;37(1):38-47
references: 17379848
references: J Immunol. 1999 Feb 1;162(3):1227-31
references: 9973373
references: Am J Hum Genet. 2002 Jan;70(1):230-6
references: 11709756
references: J Allergy Clin Immunol. 2010 Dec;126(6):1218-25.e10
references: 20947153
references: Allergol Int. 2006 Jun;55(2):121-9
references: 17075248
references: Prog Retin Eye Res. 2006 Mar;25(2):165-87
references: 16427347
references: Brain Res Bull. 2010 Feb 15;81(2-3):219-28
references: 19828129
references: J Allergy Clin Immunol. 2003 Mar;111(3):520-6
references: 12642832
references: Biochem Biophys Res Commun. 2006 Aug 18;347(1):238-47
references: 16824488
references: Invest Ophthalmol Vis Sci. 2002 Jan;43(1):183-8
references: 11773030
references: J Allergy Clin Immunol. 2009 Feb;123(2):466-71
references: 18996575
references: Br J Ophthalmol. 2011 Jun;95(6):859-63
references: 21349943
references: Cornea. 2008 Sep;27 Suppl 1:S31-40
references: 18813073
references: Biochem Biophys Res Commun. 2011 Jan 7;404(1):419-23
references: 21130742
references: J Biol Chem. 2003 Dec 26;278(52):52792-801
references: 14532295
references: Arch Biochem Biophys. 2008 Mar 1;471(1):85-93
references: 18158910
references: Am J Respir Cell Mol Biol. 2007 Jan;36(1):20-31
references: 16873769
references: Nat Rev Immunol. 2007 Jun;7(6):454-65
references: 17525754