title: Pharmacological effect of carvacrol on D: -galactosamine-induced mitochondrial enzymes and DNA damage by single-cell gel electrophoresis.
abstract: The present study aimed at investigating the effect of carvacrol on hepatic mitochondrial enzyme activities and DNA damage in D: -galactosamine (D: -GalN)-induced hepatotoxicity in male albino Wistar rats. The activities of hepatic mitochondrial enzymes such as isocitrate dehydrogenase, α-ketoglutarate dehydrogenase, succinate dehydrogenase, malate dehydrogenase, NADPH dehydrogenase and cytochrome c oxidase significantly decreased in D: -GalN-hepatotoxic rats, and administration of carvacrol brought these parameters towards normality. In D: -GalN-hepatotoxic rats, the hepatic mitochondrial concentration of thiobarbituric acid reactive substances significantly increased, and administration of carvacrol significantly reduced them towards normality. Furthermore, the activities of enzymatic antioxidants such as superoxide dismutase and glutathione peroxidase and the levels of non-enzymatic antioxidants such as vitamin C, vitamin E and reduced glutathione decreased significantly in the liver mitochondria. Administration of carvacrol returned the enzymatic and non-enzymatic antioxidants towards normality. D: -GalN-hepatotoxic rats had increased DNA damage, which administration of carvacrol significantly decreased. These results suggest that carvacrol has liver mitochondrial antioxidant properties and possesses a defensive effect against mitochondrial enzymes and DNA damage in D: -GalN-induced rats.
authors: Department of Food Science and Nutrition, King Saud University, Riyadh, Saudi Arabia.
authors: Balakrishnan
authors: B
authors: Aristatile
authors: Khalid S
authors: KS
authors: Al-Numair
authors: Abdullah H
authors: AH
authors: Al-Assaf
authors: Kodukkur Viswanathan
authors: KV
authors: Pugalendi
references: J Biol Chem. 1948 Jul;174(3):961-77
references: 18871256
references: Arch Biochem Biophys. 1959 May;82(1):70-7
references: 13650640
references: Exp Mol Pathol. 1968 Oct;9(2):279-90
references: 4952077
references: Free Radic Biol Med. 2002 Jan 1;32(1):11-6
references: 11755312
references: Diabetes. 2004 Jun;53(6):1412-7
references: 15161742
references: Acta Biochim Pol. 2001;48(1):233-40
references: 11440175
references: Cancer Lett. 1997 May 1;115(1):105-11
references: 9097985
references: J Food Prot. 2001 Oct;64(10):1542-8
references: 11601703
references: Food Chem Toxicol. 1994 Jan;32(1):31-6
references: 7510659
references: J Pharm Pharmacol. 2004 Nov;56(11):1449-55
references: 15525453
references: Life Sci. 2000;66(6):477-84
references: 10794064
references: Biochim Biophys Acta. 1995 May 24;1271(1):1-6
references: 7599195
references: Mol Genet Metab. 2003 Sep-Oct;80(1-2):11-26
references: 14567954
references: Mutat Res. 1997 Oct 6;379(2):201-10
references: 9357549
references: J Agric Food Chem. 2001 Sep;49(9):4168-70
references: 11559104
references: Fundam Clin Pharmacol. 2009 Dec;23(6):757-65
references: 19650854
references: Indian J Biochem Biophys. 1984 Apr;21(2):130-2
references: 6490072
references: Biochem J. 1952 Oct;52(2):185-96
references: 13018205
references: Am J Physiol. 1997 Jul;273(1 Pt 1):G7-17
references: 9252504
references: J Biochem Mol Toxicol. 2015 Nov;29(11):497-507
references: 26768646
references: J Ethnopharmacol. 2002 Oct;82(2-3):83-7
references: 12241981
references: Scand J Clin Lab Invest Suppl. 1968;97:77-89
references: 4179068
references: J Intern Med. 1995 Nov;238(5):405-21
references: 7595180
references: Science. 1973 Feb 9;179(4073):588-90
references: 4686466
references: Mol Cell Biochem. 1996 Dec 6;165(1):1-7
references: 8974075
references: J Biol Chem. 1962 Feb;237:569-76
references: 14474263
references: Exp Cell Res. 1988 Mar;175(1):184-91
references: 3345800
references: J Agric Food Chem. 2004 Jun 2;52(11):3309-12
references: 15161188
references: Appl Environ Microbiol. 2005 Feb;71(2):797-803
references: 15691933
references: J Biomed Sci. 2005;12(2):389-97
references: 15917992
references: Chem Biol Interact. 1990;75(2):201-11
references: 2369786
references: Science. 1992 May 1;256(5057):628-32
references: 1533953
references: Proc Natl Acad Sci U S A. 1994 Nov 8;91(23):10771-8
references: 7971961
references: Pharmacol Toxicol. 1995 Aug;77(2):81-6
references: 8584508
references: Eur J Biochem. 1968 Oct 17;6(1):126-30
references: 4387188