title: Regulation of F0F1-ATPase from Synechocystis sp. PCC 6803 by gamma and epsilon subunits is significant for light/dark adaptation.
abstract: The γ and ε subunits of F(0)F(1)-ATP synthase from photosynthetic organisms display unique properties not found in other organisms. Although the γ subunit of both chloroplast and cyanobacterial F(0)F(1) contains an extra amino acid segment whose deletion results in a high ATP hydrolysis activity (Sunamura, E., Konno, H., Imashimizu-Kobayashi, M., Sugano, Y., and Hisabori, T. (2010) Plant Cell Physiol. 51, 855-865), its ε subunit strongly inhibits ATP hydrolysis activity. To understand the physiological significance of these phenomena, we studied mutant strains with (i) a C-terminally truncated ε (ε(ΔC)), (ii) γ lacking the inserted sequence (γ(Δ198-222)), and (iii) a double mutation of (i) and (ii) in Synechocystis sp. PCC 6803. Although thylakoid membranes from the ε(ΔC) strain showed higher ATP hydrolysis and lower ATP synthesis activities than those of the wild type, no significant difference was observed in growth rate and in intracellular ATP level both under light conditions and during light-dark cycles. However, both the ε(ΔC) and γ(Δ198-222) and the double mutant strains showed a lower intracellular ATP level and lower cell viability under prolonged dark incubation compared with the wild type. These data suggest that internal inhibition of ATP hydrolysis activity is very important for cyanobacteria that are exposed to prolonged dark adaptation and, in general, for the survival of photosynthetic organisms in an ever-changing environment.
authors: Chemical Resources Laboratory, Tokyo Institute of Technology, Nagatsuta 4259-R1-8, Midori-Ku, Yokohama 226-8503, Japan.
authors: Mari
authors: M
authors: Imashimizu
authors: Gábor
authors: G
authors: Bernát
authors: Ei-ichiro
authors: E
authors: Sunamura
authors: Martin
authors: M
authors: Broekmans
authors: Hiroki
authors: H
authors: Konno
authors: Kota
authors: K
authors: Isato
authors: Matthias
authors: M
authors: Rögner
authors: Toru
authors: T
authors: Hisabori
references: FEBS Lett. 1991 Dec 9;294(3):271-4
references: 1756870
references: Plant Cell Physiol. 2008 Jan;49(1):11-8
references: 18003670
references: Nat Rev Mol Cell Biol. 2001 Sep;2(9):669-77
references: 11533724
references: Plant Cell Physiol. 2010 Jun;51(6):855-65
references: 20421199
references: Proc Natl Acad Sci U S A. 2007 Jul 3;104(27):11233-8
references: 17581881
references: Bacteriol Rev. 1971 Jun;35(2):171-205
references: 4998365
references: J Biol Chem. 1982 Feb 10;257(3):1125-7
references: 6460023
references: Plant Cell Physiol. 2008 Nov;49(11):1672-7
references: 18784196
references: J Biol Chem. 2011 Mar 18;286(11):9071-8
references: 21193405
references: Arch Microbiol. 1976 Jul;108(3):249-58
references: 821448
references: FEBS Lett. 1990 Feb 12;261(1):204-8
references: 2137788
references: Photosynth Res. 2009 Mar;99(3):205-16
references: 19137411
references: Nature. 1997 Mar 20;386(6622):299-302
references: 9069291
references: Biochim Biophys Acta. 2001 Jul 2;1506(1):31-46
references: 11418095
references: Proc Natl Acad Sci U S A. 1977 Mar;74(3):936-40
references: 139610
references: Proc Natl Acad Sci U S A. 2001 Jun 5;98(12):6560-4
references: 11381110
references: J Biol Chem. 1985 Sep 15;260(20):11200-6
references: 2863270
references: J Biol Chem. 1978 May 10;253(9):3123-8
references: 147871
references: J Biol Chem. 1984 Jun 10;259(11):7275-80
references: 6233280
references: Annu Rev Biochem. 1997;66:717-49
references: 9242922
references: J Biol Chem. 2000 Nov 17;275(46):35746-50
references: 10958801
references: Biochemistry. 1998 Nov 17;37(46):16423-9
references: 9819235
references: Biochem Biophys Res Commun. 1972 Dec 18;49(6):1617-23
references: 4404797
references: Nat Struct Biol. 1995 Nov;2(11):961-7
references: 7583669
references: J Biol Chem. 1984 Jun 25;259(12):7371-3
references: 6234301
references: Biochemistry. 2004 Mar 23;43(11):3273-9
references: 15023078
references: J Biol Chem. 1979 Oct 10;254(19):9525-33
references: 158591
references: Plant Physiol. 1992 May;99(1):153-60
references: 16668843
references: J Biol Chem. 2006 Jan 6;281(1):501-7
references: 16267041
references: Photosynth Res. 2008 May;96(2):153-62
references: 18365763
references: J Bacteriol. 2003 Sep;185(18):5527-35
references: 12949105
references: J Biol Chem. 1984 Sep 10;259(17):11121-8
references: 6236222
references: Proc Natl Acad Sci U S A. 2009 Aug 18;106(33):14168-73
references: 19666549
references: FEBS Lett. 2003 Jun 12;545(1):71-5
references: 12788494
references: J Biol Chem. 1988 Nov 25;263(33):17437-42
references: 2903163
references: Biochemistry. 2002 Dec 24;41(51):15130-4
references: 12484749
references: J Biol Chem. 1999 Jul 2;274(27):19124-8
references: 10383416
references: J Bacteriol. 1989 Jun;171(6):3449-57
references: 2498291
references: Biochem J. 2009 Dec 14;425(1):85-94
references: 19785575
references: Mol Gen Genet. 1990 Apr;221(2):155-63
references: 2196429
references: EMBO J. 2006 Oct 4;25(19):4596-604
references: 16977308
references: Biochim Biophys Acta. 2006 May-Jun;1757(5-6):326-38
references: 16701076