title: ODAM Expression Inhibits Human Breast Cancer Tumorigenesis.
abstract: We have posited that Odontogenic Ameloblast Associated Protein (ODAM) serves as a novel prognostic biomarker in breast cancer and now have investigated its potential role in regulating tumor growth and metastasis. Human breast cancer MDA-MB-231 cells were transfected with a recombinant ODAM plasmid construct (or, as a control, the plasmid vector alone). ODAM expression increased adhesion and apoptosis of the transfected MDA-MB-231 cells and suppressed their growth rate, migratory activity, and capability to invade extracellular matrix-coated membranes. Implantation of such cells into mouse mammary fat pads resulted in significantly smaller tumors than occurred in animals that received control cells; furthermore, ODAM-expressing cells, when injected intravenously into mice, failed to metastasize, whereas the control-transfected counterparts produced extensive lung lesions. Our finding that induction of ODAM expression in human breast cancer cells markedly inhibited their neoplastic properties provides further evidence for the regulatory role of this molecule in tumorigenesis and, consequently, is of potential clinical import.
authors: Human Immunology and Cancer Program/Department of Medicine.
authors: Daniel P
authors: DP
authors: Kestler
authors: James S
authors: JS
authors: Foster
authors: Charles T
authors: CT
authors: Bruker
authors: John W
authors: JW
authors: Prenshaw
authors: Stephen J
authors: SJ
authors: Kennel
authors: Jonathan S
authors: JS
authors: Wall
authors: Deborah T
authors: DT
authors: Weiss
authors: Alan
authors: A
authors: Solomon
references: Lab Invest. 2009 Nov;89(11):1229-42
references: 19770839
references: Cancer Res. 2008 Oct 1;68(19):7795-802
references: 18829534
references: Int J Oncol. 2009 Apr;34(4):881-95
references: 19287945
references: Am Surg. 2009 Sep;75(9):769-75; discussion 775
references: 19774947
references: J Cell Sci. 2007 May 1;120(Pt 9):1505-11
references: 17452622
references: Clin Cancer Res. 2004 Jan 15;10(2):591-7
references: 14760081
references: Cancer Res. 2004 Jul 1;64(13):4506-13
references: 15231660
references: J Cell Biochem. 2009 Oct 1;108(2):378-87
references: 19585539
references: Mol Med. 2008 May-Jun;14(5-6):318-26
references: 18472969
references: Blood Cells Mol Dis. 2010 Jun 15;45(1):82-5
references: 20362478
references: J Cell Biochem. 2010 Oct 15;111(3):755-67
references: 20665536
references: Eur J Oral Sci. 2007 Apr;115(2):153-60
references: 17451507
references: Breast Cancer. 2008;15(1):31-4
references: 18224391
references: Am J Pathol. 2007 Jun;170(6):2112-21
references: 17525277
references: Amyloid. 2008 Jun;15(2):89-95
references: 18484335
references: J Natl Cancer Inst. 2006 Jul 19;98(14):948-51
references: 16849671
references: Endocrinology. 2006 Nov;147(11):5023-33
references: 16873542
references: Nature. 2003 Mar 20;422(6929):313-7
references: 12629553
references: J Cell Physiol. 2011 Feb;226(2):375-84
references: 20665703
references: Mol Biol Cell. 2007 Jul;18(7):2411-8
references: 17442891
references: Cell Motil Cytoskeleton. 2009 Dec;66(12):1048-56
references: 19593789
references: Eur Cell Mater. 2010 Dec 10;20:393-402
references: 21154245
references: Oncogene. 2005 Dec 15;24(56):8277-90
references: 16116478
references: Nat Protoc. 2007;2(3):504-11
references: 17406614
references: Cancer Res. 2008 Apr 1;68(7):2194-203
references: 18381425
references: Breast Cancer Res. 2010;12(5):R89
references: 21029421
references: Mol Cell Biol. 2008 Jan;28(2):642-55
references: 18039857
references: Breast Cancer Res Treat. 2010 Aug;123(1):59-71
references: 19902354
references: J Cell Sci. 2007 Oct 15;120(Pt 20):3491-9
references: 17928305
references: Proc Natl Acad Sci U S A. 2005 Feb 1;102(5):1454-9
references: 15665096
references: J Periodontal Res. 2010 Dec;45(6):709-13
references: 20572917
references: Breast Cancer Res Treat. 1994;31(2-3):325-35
references: 7881109
references: Oncogene. 2008 Feb 21;27(9):1320-6
references: 17724469
references: Oncogene. 2006 Apr 20;25(17):2546-57
references: 16331256
references: Int J Cancer. 2009 Sep 15;125(6):1343-51
references: 19533747
references: J Cancer Res Clin Oncol. 2007 Oct;133(10):761-9
references: 17486367
references: J Cell Biochem. 2008 Feb 15;103(3):941-56
references: 17647262
references: BMC Cancer. 2009 Aug 25;9:298
references: 19706168
references: Cancer Res. 2009 Sep 1;69(17):6807-14
references: 19690135
references: J Biomed Biotechnol. 2010;2010:402593
references: 20224657
references: Mol Cancer Res. 2005 Jul;3(7):365-71
references: 16046547
references: J Lab Clin Med. 2003 Nov;142(5):348-55
references: 14647039
references: Eur J Oral Sci. 2006 May;114 Suppl 1:139-46; discussion 164-5, 380-1
references: 16674676
references: Breast Cancer Res. 2009;11(3):R32
references: 19473496
references: Biochim Biophys Acta. 2010 Jul;1803(7):786-95
references: 20307590
references: Arthritis Rheum. 2006 Nov;54(11):3545-50
references: 17075859
references: J Cell Biochem. 2009 May 15;107(2):194-202
references: 19330799
references: J Cell Sci. 1998 Jul 30;111 ( Pt 14):1973-80
references: 9645945
references: Oncogene. 2003 Apr 3;22(13):2021-33
references: 12673207
references: EMBO J. 2007 Jun 6;26(11):2633-44
references: 17491594
references: Eur J Cancer. 2004 Dec;40(18):2717-25
references: 15571953
references: Mol Cell Biol. 2001 Feb;21(3):794-810
references: 11154267