title: Inhibition of K+ secretion in the distal nephron in nephrotic syndrome: possible role of albuminuria.
abstract: Nephrotic syndrome features massive proteinuria and retention of sodium which promotes ascite formation. In the puromycin aminonucleoside-induced rat model of nephrotic syndrome, sodium retention originates from the collecting duct where it generates a driving force for potassium secretion. However, there is no evidence for urinary potassium loss or hypokalaemia in the nephrotic syndrome. We therefore investigated the mechanism preventing urinary potassium loss in the nephrotic rats and, for comparison, in hypovolaemic rats, another model displaying increased sodium reabsorption in collecting ducts. We found that sodium retention is not associated with urinary loss of potassium in either nephrotic or hypovolaemic rats, but that different mechanisms account for potassium conservation in the two models. Collecting ducts from hypovolaemic rats displayed high expression of the potassium-secreting channel ROMK but no driving force for potassium secretion owing to low luminal sodium availability. In contrast, collecting ducts from nephrotic rats displayed a high driving force for potassium secretion but no ROMK. Down-regulation of ROMK in nephrotic rats probably stems from phosphorylation of ERK arising from the presence of proteins in the luminal fluid. In addition, nephrotic rats displayed a blunted capacity to excrete potassium when fed a potassium-rich diet, and developed hyperkalaemia. As nephrotic patients were found to display plasma potassium levels in the normal to high range, we would recommend not only a low sodium diet but also a controlled potassium diet for patients with nephrotic syndrome.
authors: UPMC University of Paris 06, and INSERM UMRS 872 team 3, and CNRS ERL 7226, Centre de recherche des Cordeliers, Paris, France.
authors: Marc
authors: M
authors: Fila
authors: Gaëlle
authors: G
authors: Brideau
authors: Luciana
authors: L
authors: Morla
authors: Lydie
authors: L
authors: Cheval
authors: Georges
authors: G
authors: Deschênes
authors: Alain
authors: A
authors: Doucet
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