title: [Cloning of the glyceraldehydes 3-phosphate dehydrogenase gene of porphyromonas gingivalis and its expression in E. coli].
abstract: OBJECTIVE
  To clone the glyceraldehydes 3-phosphate dehydrogenase (GAPDH) gene of Porphyromonas gingivalis (P. gingivalis) and to induce its fusion expression in E. coli.
  
  
  METHODS
  GAPDH was obtained by PCR and was inserted into cloning vector pMD-18-T to construct clone recon. Double enzymes digest the recon pMD18-T-GAPDH and the prokaryotic expression vector pET-32a and then connect to get the expressing recon pET-32a-GAPDH. The recombinant expression plasmid which had been confirmed by enzymes digestion was transformed to E. coli competent cells BL21 and induced the expression of GAPDH with isopropyl beta-D-1-thiogalactopyranoside (IPTG) of different density.
  
  
  RESULTS
  DNA sequencing showed that the fragment was 99.802% the same to the sequence published in NCBI. Under the best density, IPTG could be highly expressed.
  
  
  CONCLUSIONS
  The GAPDH had been successfully cloned and expressed in E. coli which gets ready for the following experiment to study the immunity of GAPDH and the homologues antibody preparation.
authors: Research Center for Stomatology, Stomatological Hospital, College of Medicine, Xi'an Jiaotong University, Xi'an 710004, China.
authors: Ang
authors: A
authors: Li
authors: Hong-Yan
authors: HY
authors: Xu
authors: Jian-Feng
authors: JF
authors: Shi
authors: Chun-Hui
authors: CH
authors: Zhu
authors: Guo-Zhou
authors: GZ
authors: Rao
authors: Jian-Zhong
authors: JZ
authors: Gou