title: p53 regulates Ki-67 promoter activity through p53- and Sp1-dependent manner in HeLa cells.
abstract: The expression of the human Ki-67 protein, which is strictly associated with cell proliferation, is regulated by a variety of cellular mediators. In this study, we studied the effects of p53 on Ki-67 promoter in HeLa cells using luciferase reporter assay. The results showed that: (1) p53 inhibited Ki-67 promoter activity in a dose-dependent manner, (2) the p53-binding motifs mediated part of the transcriptional repression of Ki-67 promoter through a sequence-specific interaction with p53, (3) p53 was able to repress the Sp1-stimulated Ki-67 promoter activity, and (4) the Sp1-binding sites were responsible for the p53-mediated transcriptional repression of Ki-67 promoter. In conclusion, p53 inhibited Ki-67 promoter activity via p53- and Sp1-dependent pathways, and the interaction between p53 and Sp1 might be involved in the transcriptional regulatory mechanisms.
authors: Jiangsu Key Laboratory of Biological Cancer Therapy, Xuzhou Medical College, 84 West Huai-hai Road, Xuzhou, 221002, Jiangsu, People's Republic of China.
authors: Mei-Juan
authors: MJ
authors: Wang
authors: Dong-Sheng
authors: DS
authors: Pei
authors: Guo-Wei
authors: GW
authors: Qian
authors: Xiao-Xing
authors: XX
authors: Yin
authors: Qian
authors: Q
authors: Cheng
authors: Lian-Tao
authors: LT
authors: Li
authors: Hui-Zhong
authors: HZ
authors: Li
authors: Jun-Nian
authors: JN
authors: Zheng
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