title: Orthogonal gene expression in Escherichia coli.
abstract: Here, we describe a route orthogonal gene expression which combines orthogonal transcription and translation using library-based selections. We show how orthogonal gene expression can be used to create a minimal orthogonal ribosome and describe how to create orthogonal transcription-translation feed forward loops that introduce tailored information processing delays into gene expression.
authors: Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge, United Kingdom.
authors: Wenlin
authors: W
authors: An
authors: Jason W
authors: JW
authors: Chin