title: Development and evaluation of formulations of microbial biotransformed extract of tobacco leaves for hair growth potential.
abstract: BACKGROUND
  Extensive researches are going on to explore the effective and safe drug for their hair growth. Tobacco leaves are traditionally known to potentiate hair growth promotion. Therefore, the aim of present study was to formulate and evaluate the microbial biotransformed extract of tobacco leaves for hair growth potential in male albino wister rats.
  
  
  METHODS
  The extract of was prepared by microbial biotransformation of tobacco leaves in cow urine for 28 days. The herbal formulations (lotion) were formulated by general method using o/w type base in various rations or concentrations such as 10%, 20% and 30% of extract. These lotions were applied on shaved skin area of rats for 30 days once in a day and hair length, serum total protein, and total testosterone were measured.
  
  
  RESULTS
  Our formulations show increase in hair growth and serum total protein at concentration dependent manner with effect to standard and control groups. Serum total testosterone decreases according to a concentration dependent manner.
  
  
  CONCLUSIONS
  Further, series of investigations are, however, necessary to remain exploration, which includes their structural elucidation, characterization, clinical safety, reliability and molecular mechanism involved in this pharmacological activity.
authors: Department of Pharmacognosy, J. L. Chaturvedi College of Pharmacy, Nagpur-440016 (M.S), India.
authors: Ashlesh V
authors: AV
authors: Murkute
authors: Mahesh S
authors: MS
authors: Sahu
authors: Prashant Y
authors: PY
authors: Mali
authors: Vinod D
authors: VD
authors: Rangari
references: Phytother Res. 2003 Aug;17(7):797-800
references: 12916080
references: Postgrad Med. 2000 Jun;107(7):81-5, 89-90
references: 10887448
references: Acta Derm Venereol. 1998 Nov;78(6):428-32
references: 9833041
references: J Ethnopharmacol. 2003 Oct;88(2-3):235-9
references: 12963149
references: Br J Dermatol. 2002 Jan;146(1):41-51
references: 11841365
references: Proc Soc Exp Biol Med. 1962 Dec;111:544-7
references: 14001233
references: Am J Clin Dermatol. 2003;4(11):757-70
references: 14572298
references: Cancer Nurs. 1984 Dec;7(6):499-503
references: 6568869
references: N Engl J Med. 1996 Mar 14;334(11):707-14
references: 8594431
references: N Engl J Med. 1999 Sep 23;341(13):964-73
references: 10498493
references: J Physiol Pharmacol. 2005 Mar;56 Suppl 1:51-67
references: 15800385
references: Phytother Res. 2003 Nov;17(9):987-1000
references: 14595575
references: J Ethnopharmacol. 1986 Jun;16(2-3):117-51
references: 3528673
references: Expert Opin Pharmacother. 2004 Apr;5(4):933-40
references: 15102575
references: J Am Acad Dermatol. 2000 Apr;42(4):549-66; quiz 567-70
references: 10727299
references: Cancer Nurs. 1979 Oct;2(5):365-9
references: 261039