title: Genetic and physiological analysis of the relationship between partial resistance to clubroot and tolerance to trehalose in Arabidopsis thaliana.
abstract: In Arabidopsis thaliana the induction of plant trehalase during clubroot disease was proposed to act as a defense mechanism in the susceptible accession Col-0, which could thereby cope with the accumulation of pathogen-synthesized trehalose. In the present study, we assessed trehalose activity and tolerance to trehalose in the clubroot partially resistant accession Bur-0. We compared both accessions for several trehalose-related physiological traits during clubroot infection. A quantitative trait loci (QTLs) analysis of tolerance to exogenous trehalose was also conducted on a Bur-0xCol-0 RIL progeny. Trehalase activity was not induced by clubroot in Bur-0 and the inhibition of trehalase by validamycin treatments resulted in the enhancement of clubroot symptoms only in Col-0. In pathogen-free cultures, Bur-0 showed less trehalose-induced toxicity symptoms than Col-0. A QTL analysis identified one locus involved in tolerance to trehalose overlapping the confidence interval of a QTL for resistance to Plasmodiophora brassicae. This colocalization was confirmed using heterogeneous inbred family (HIF) lines. Although not based on trehalose catabolism capacity, partial resistance to clubroot is to some extent related to the tolerance to trehalose accumulation in Bur-0. These findings support an original model where contrasting primary metabolism-related regulations could contribute to the partial resistance to a plant pathogen.
authors: Université de Rennes 1, UMR 118, F-35042 Rennes, France.
authors: Antoine
authors: A
authors: Gravot
authors: Agrocampus Ouest, UMR 118, F-35042 Rennes, France.
authors: Louis
authors: L
authors: Grillet
authors: INRA, UMR 118, F-35042 Rennes, France.
authors: Geoffrey
authors: G
authors: Wagner
authors: INRA, UMR 118, F-35042 Rennes, France.
authors: Mélanie
authors: M
authors: Jubault
authors: INRA, UMR 118, F-35042 Rennes, France.
authors: Christine
authors: C
authors: Lariagon
authors: INRA, UMR 118, F-35042 Rennes, France.
authors: Cécile
authors: C
authors: Baron
authors: Université de Rennes 1, UMR 118, F-35042 Rennes, France.
authors: Carole
authors: C
authors: Deleu
authors: INRA, UMR 118, F-35042 Rennes, France.
authors: Régine
authors: R
authors: Delourme
authors: Université de Rennes 1, UMR 118, F-35042 Rennes, France.
authors: Alain
authors: A
authors: Bouchereau
authors: Agrocampus Ouest, UMR 118, F-35042 Rennes, France.
authors: Maria J
authors: MJ
authors: Manzanares-Dauleux
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