title: A centrifugation-free high-throughput protein purification system using in-line microfluidization.
abstract: The majority of current high-throughput protein purification protocols include rate-limiting centrifugation steps. A column and nozzle assembly was developed that can be used in-line with microfluidization for the purification of bacterially-overexpressed, His-tagged proteins directly from bacterial cultures. Yields and purity are comparable with standard protocols. This large-scale protein purification protocol is easy to use and widely-applicable.
authors: Structural Genomics Consortium, University of Toronto, 101 College St., Toronto, MaRS South Tower, Suite 700, Ontario, Canada M5G IL7.
authors: Denis
authors: D
authors: Alenkin
authors: Laila
authors: L
authors: Yermekbayeva
authors: Shariq
authors: S
authors: Mujib
authors: Anders
authors: A
authors: Vesterberg
authors: Elena
authors: E
authors: Newman
authors: Ken
authors: K
authors: Yamazaki
authors: Doug
authors: D
authors: Cossar
authors: Sirano
authors: S
authors: Dhe-Paganon