title: Human and Xenopus mo15 messenger-RNA are highly conserved but show different patterns of expression in adult tissues.
abstract: Phosphorylation of human p34(cdc2) at Thr 161 seems to be necessary for its catalytic activity. CAK (cdk activating kinase) containing p40(MO15) from Xenopus egg extracts phosphorylates and activates p34(cdc2) in a cyclin dependent manner at Thr 161. We describe the cDNA sequence coding for human MO15, which predicts a serine/threonine kinase of 346 aa. Despite the high homology of 91% between the human and Xenopus proteins we observed a rather different mRNA distribution in adult tissues: In contrast to ubiquitously expressed human MO15-transcripts MO15-mRNA expression in Xenopus is restricted to oocytes indicating a different cellular role in these two phylogenetically distant species. By virtue of the homology to members of the family of cell cycle kinase genes we examined MO15 mRNA expression for its correlation to the proliferative activity of cells. Stimulation of lymphocytes showed MO15 mRNA expression to be independent of mitotic activity.
authors: GEORG SPEYER HAUS,CHEMOTHERAPEUT FORSCHUNGSINST,D-60596 FRANKFURT,GERMANY.
authors: D
authors: D
authors: Kobelt
authors: T
authors: T
authors: Karn
authors: B
authors: B
authors: Hock
authors: U
authors: U
authors: Holtrich
authors: A
authors: A
authors: Brauninger
authors: G
authors: G
authors: Wolf
authors: K
authors: K
authors: Strebhardt
authors: K
authors: K
authors: Rubsamenwaigmann