title: Chromatin "prepattern" and histone modifiers in a fate choice for liver and pancreas.
abstract: Transcriptionally silent genes can be marked by histone modifications and regulatory proteins that indicate the genes' potential to be activated. Such marks have been identified in pluripotent cells, but it is unknown how such marks occur in descendant, multipotent embryonic cells that have restricted cell fate choices. We isolated mouse embryonic endoderm cells and assessed histone modifications at regulatory elements of silent genes that are activated upon liver or pancreas fate choices. We found that the liver and pancreas elements have distinct chromatin patterns. Furthermore, the histone acetyltransferase P300, recruited via bone morphogenetic protein signaling, and the histone methyltransferase Ezh2 have modulatory roles in the fate choice. These studies reveal a functional "prepattern" of chromatin states within multipotent progenitors and potential targets to modulate cell fate induction.
authors: Institute for Regenerative Medicine, Epigenetics Program, Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA.
authors: Cheng-Ran
authors: CR
authors: Xu
authors: Philip A
authors: PA
authors: Cole
authors: David J
authors: DJ
authors: Meyers
authors: Jay
authors: J
authors: Kormish
authors: Sharon
authors: S
authors: Dent
authors: Kenneth S
authors: KS
authors: Zaret
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