title: Dehydrogenase GRD1 represents a novel component of the cellulase regulon in Trichoderma reesei (Hypocrea jecorina).
abstract: Trichoderma reesei (Hypocrea jecorina) is nowadays the most important industrial producer of cellulase and hemicellulase enzymes, which are used for pretreatment of cellulosic biomass for biofuel production. In this study, we introduce a novel component, GRD1 (glucose-ribitol dehydrogenase 1), which shows enzymatic activity on cellobiose and positively influences cellulase gene transcription, expression, and extracellular endo-1,4-β-D-glucanase activity. grd1 is differentially transcribed upon growth on cellulose and the induction of cellulase gene expression by sophorose. The transcription of grd1 is coregulated with that of cel7a (cbh1) under inducing conditions. GRD1 is further involved in carbon source utilization on several carbon sources, such as those involved in lactose and D-galactose catabolism, in several cases in a light-dependent manner. We conclude that GRD1 represents a novel enhancer of cellulase gene expression, which by coregulation with the major cellulase may act via optimization of inducing mechanisms.
authors: Research Area of Gene Technology and Applied Biochemistry, Institute for Chemical Engineering, Vienna University of Technology, Gumpendorfer Strasse 1a/1665, A-1060 Vienna, Austria.
authors: André
authors: A
authors: Schuster
authors: Christian P
authors: CP
authors: Kubicek
authors: Monika
authors: M
authors: Schmoll
references: J Bacteriol. 1957 Feb;73(2):269-78
references: 13416182
references: Mol Microbiol. 1995 May;16(4):687-97
references: 7476163
references: Arch Microbiol. 1984 Jan;137(1):53-7
references: 6538780
references: J Bacteriol. 1980 Dec;144(3):1197-9
references: 6777367
references: Science. 2001 Sep 14;293(5537):2087-92
references: 11557892
references: BMC Microbiol. 2008 Oct 08;8:174
references: 18842142
references: J Gen Microbiol. 1987 Jun;133(6):1481-7
references: 3117961
references: Fungal Genet Biol. 2009 Mar;46 Suppl 1:S2-13
references: 19146970
references: Annu Rev Phytopathol. 2005;43:437-58
references: 16078891
references: Nature. 2003 Apr 24;422(6934):859-68
references: 12712197
references: Eukaryot Cell. 2008 Jul;7(7):1227-30
references: 18503005
references: J Bacteriol. 1979 Sep;139(3):761-9
references: 39061
references: FEMS Microbiol Lett. 2006 Jan;254(2):240-4
references: 16445751
references: Curr Genet. 2005 Aug;48(2):126-33
references: 15959723
references: Fungal Genet Biol. 2010 Dec;47(12):962-72
references: 20816830
references: Fungal Genet Biol. 2004 Sep;41(9):877-87
references: 15288024
references: Fungal Genet Biol. 2010 May;47(5):468-76
references: 20144726
references: BMC Biol. 2009 Sep 03;7:58
references: 19728862
references: Bioinformatics. 2007 Nov 1;23(21):2947-8
references: 17846036
references: Appl Microbiol Biotechnol. 2010 Feb;85(5):1259-77
references: 19915832
references: Biochim Biophys Acta. 1989 Jan 27;990(1):1-7
references: 2914146
references: Science. 2000 Dec 15;290(5499):2110-3
references: 11118138
references: J Bacteriol. 1962 Feb;83:400-8
references: 14469205
references: J Biol Chem. 2007 Jun 22;282(25):18654-18659
references: 17452322
references: Nucleic Acids Res. 2005 Nov 14;33(20):6469-85
references: 16287898
references: Mol Microbiol. 1992 Nov;6(21):3225-35
references: 1453960
references: Gene. 1985;33(1):103-19
references: 2985470
references: Arch Microbiol. 1991;155(6):601-6
references: 1953300
references: J Am Chem Soc. 2009 Mar 4;131(8):2965-70
references: 19199437
references: Appl Environ Microbiol. 2008 Dec;74(24):7607-12
references: 18978088
references: Biotechnol Biofuels. 2009 Sep 01;2:19
references: 19723296
references: Eur J Biochem. 2004 May;271(10):1864-72
references: 15128296
references: Proc Natl Acad Sci U S A. 1989 Aug;86(16):6138-41
references: 2762318
references: Mol Microbiol. 2007 Nov;66(4):890-900
references: 17924946
references: FEBS Lett. 2009 Apr 17;583(8):1309-13
references: 19303876
references: J Biotechnol. 2009 Jan 15;139(2):146-51
references: 19027803
references: J Bacteriol. 1988 Aug;170(8):3689-93
references: 3403510
references: J Bacteriol. 1960 Jun;79:816-26
references: 14420566
references: Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14863-8
references: 9843981
references: Curr Genet. 1990 Jul;18(1):71-6
references: 2245476
references: Appl Environ Microbiol. 1981 Apr;41(4):924-31
references: 16345755
references: Proc Natl Acad Sci U S A. 1996 Dec 10;93(25):14873-7
references: 8962148
references: Mol Biol Evol. 2007 Aug;24(8):1596-9
references: 17488738
references: PLoS One. 2008 Aug 29;3(8):e3105
references: 18769678
references: Appl Environ Microbiol. 2006 Mar;72(3):2126-33
references: 16517662
references: Acta Microbiol Immunol Hung. 2003;50(2-3):125-45
references: 12894484
references: Chem Biol. 2008 Jun;15(6):527-32
references: 18559263
references: Mol Microbiol. 2010 Sep;77(6):1483-501
references: 20735770
references: Eukaryot Cell. 2005 Dec;4(12):1998-2007
references: 16339718
references: Nat Biotechnol. 2008 May;26(5):553-60
references: 18454138
references: PLoS One. 2010 Feb 12;5(2):e9191
references: 20169200
references: Curr Opin Biotechnol. 2003 Aug;14(4):438-43
references: 12943855
references: Eukaryot Cell. 2009 Mar;8(3):410-20
references: 19136572
references: Proc Natl Acad Sci U S A. 2000 Nov 7;97(23):12684-9
references: 11058161
references: Proc Natl Acad Sci U S A. 2009 Dec 29;106(52):22157-62
references: 20018766
references: Anal Biochem. 1987 Apr;162(1):156-9
references: 2440339