title: Role of heme oxygenase-1 in inflammatory response induced by mechanical stretch in synovial cells.
abstract: OBJECTIVE
  The purpose of this study was to investigate the mechanism by which heme oxygenase-1 (HO-1) regulates inflammatory responses induced by mechanical stretch in human fibroblast-like synoviocyte (HFLS) cells.
  
  
  METHODS
  HFLS cells were cultured in the presence of hemin and seeded into fibronectin-coated silicon chambers. The chambers were attached to a stretching apparatus which applied a uniaxial sinusoidal stretching force. The genetic expressions of cyclooxygenase-2 (COX-2), interleukin-1β (IL-1β) and HO-1 were analyzed using real-time RT-PCR. The expression and localization of HO-1 protein were detected by immunofluorescence staining. The amounts of prostaglandin E(2) (PGE(2)) released into the culture medium were determined using ELISA.
  
  
  RESULTS
  Mechanical stretch enhanced the expressions of COX-2 and IL-1β, and the amount of PGE(2) synthesis in HFLS cells, whereas that of HO-1 was slightly increased. In contrast, treatment with hemin enhanced HO-1 gene expression and mechanical stretch enhanced this expression in hemin-pretreated cells. In addition, hemin pretreatment suppressed PGE(2) synthesis induced by mechanical stretch.
  
  
  CONCLUSIONS
  We found that constitutive HO-1 expression in hemin-pretreated HFLS cells suppressed mechanical stretch-induced inflammatory responses, suggesting that HO-1 may play a role as a regulation factor in synovial tissue inflammation.
authors: Division of Infection and Molecular Biology, Department of Health Promotion, Kyushu Dental College, 2-6-1 Manazuru Kokurakita-ku, Kitakyushu, Fukuoka, 803-8580, Japan.
authors: Masanobu
authors: M
authors: Takao
authors: Toshinori
authors: T
authors: Okinaga
authors: Wataru
authors: W
authors: Ariyoshi
authors: Kenjiro
authors: K
authors: Iwanaga
authors: Ikuo
authors: I
authors: Nakamichi
authors: Izumi
authors: I
authors: Yoshioka
authors: Kazuhiro
authors: K
authors: Tominaga
authors: Tatsuji
authors: T
authors: Nishihara
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