title: Treatment of sperm with extracellular adenosine 5'-triphosphate improves the in vitro fertility rate of inbred and genetically modified mice with low fertility.
abstract: In vitro fertilization (IVF) is one of the most important techniques used for assisted reproduction in mouse colony management. As with natural mating, where mice have varying fertility indices, fertility rates of genetically modified (GM) [transgenic (Tg), knock out (KO) and congenic (Cg)] mice are influenced by their genetic background. Lines of GM mice that have poor fertility have a concomitant poor IVF outcome. Treatment of mouse sperm with extracellular adenosine 5'-triphosphate (ATPe) enhanced in vitro fertilization rates in outbred and hybrid mice. The objective of this study was to analyze the effects of using extracellular adenosine 5'-triphosphate-treated sperm for IVF of inbred wild type, and genetically modified mouse lines, for which standard IVF did not work well. The IVF was performed using the GM mice on C57BL/10SnJ, C57BL/6J, BALB/cJ and NFS/N background strains and wild type (WT) mice such as C57BL/6N, BALB/cAnN, and B6129SF1 strains. Oocytes from superovulated females were fertilized in vitro with sperm from the same background strain, and either treated or not treated with ATPe. The ATPe treatment enhanced IVF outcome in most of the GM and some WT strains, as indicated by the percentage of embryos that progressed to the two-cell stage. There was no marked difference between ATPe treated and control groups for the development rate of two-cell embryos to blastocysts in culture, or in the number of pups born after transfer of two-cell embryos into recipient females. The observed improvement of the IVF results following ATPe treatment of transgenic and KO mouse sperm were a potential solution for improving the outcome of assisted reproduction techniques used for rederivation or for gamete banking.
authors: Assisted Reproduction Technologies and Cryopreservation Unit, CMB/National Institute of Allergy and Infectious Diseases/ National Institutes of Health, Rockville, Maryland, USA.
authors: Kuzhalini
authors: K
authors: Vasudevan
authors: Jorge M
authors: JM
authors: Sztein
references: Biol Reprod. 2000 Dec;63(6):1774-80
references: 11090448
references: Fertil Steril. 2004 Jul;82(1):52-6
references: 15236989
references: Biol Reprod. 2008 Jul;79(1):164-71
references: 18401012
references: Theriogenology. 2006 Jun;65(9):1716-26
references: 16271754
references: Biol Reprod. 2002 Jul;67(1):287-92
references: 12080030
references: J Reprod Fertil. 1986 Nov;78(2):515-27
references: 2433439
references: Biochem Biophys Res Commun. 1982 Aug 31;107(4):1326-32
references: 7138541
references: Biol Reprod. 1976 Dec;15(5):647-53
references: 1000005
references: Biol Reprod. 1983 Apr;28(3):683-90
references: 6405817
references: Reprod Sci. 2007 Oct;14(7):655-66
references: 18000227
references: Mol Reprod Dev. 2000 Jul;56(3):436-40
references: 10862011
references: J Reprod Fertil. 1990 Sep;90(1):147-56
references: 2231536
references: J Reprod Fertil. 1977 Jan;49(1):83-7
references: 833793
references: Biol Reprod. 1999 Sep;61(3):809-12
references: 10456861
references: Transgenic Res. 2010 Aug;19(4):587-94
references: 19844803
references: J Reprod Dev. 2006 Aug;52(4):561-8
references: 16757880
references: Biol Reprod. 1998 Dec;59(6):1328-33
references: 9828175
references: J Exp Zool. 1976 Jul;197(1):13-9
references: 939958
references: J Reprod Fertil. 1971 Aug;26(2):197-208
references: 5105157
references: Biol Reprod. 2002 Feb;66(2):429-37
references: 11804959
references: Biol Reprod. 2009 Jan;80(1):124-33
references: 18799757
references: Fertil Steril. 1995 Jan;63(1):166-75
references: 7805907
references: J Biol Chem. 1992 Sep 25;267(27):19443-7
references: 1527064
references: Hum Reprod. 1999 Mar;14(3):694-7
references: 10221697
references: Am J Physiol. 1993 Sep;265(3 Pt 1):C577-606
references: 8214015
references: J Exp Zool. 1981 Jan;215(1):35-46
references: 6785374