title: A Shigella boydii bacteriophage which resembles Salmonella phage ViI.
abstract: BACKGROUND
  Lytic bacteriophages have been applied successfully to control the growth of various foodborne pathogens. Sequencing of their genomes is considered as an important preliminary step to ensure their safety prior to food applications.
  
  
  RESULTS
  The lytic bacteriophage, ΦSboM-AG3, targets the important foodborne pathogen, Shigella. It is morphologically similar to phage ViI of Salmonella enterica serovar Typhi and a series of phages of Acinetobacter calcoaceticus and Rhizobium meliloti. The complete genome of ΦSboM-AG3 was determined to be 158 kb and was terminally redundant and circularly permuted. Two hundred and sixteen open reading frames (ORFs) were identified and annotated, most of which displayed homology to proteins of Salmonella phage ViI. The genome also included four genes specifying tRNAs.
  
  
  CONCLUSIONS
  This is the first time that a Vi-specific phage for Shigella has been described. There is no evidence for the presence of virulence and lysogeny-associated genes. In conclusion, the genome analysis of ΦSboM-AG3 indicates that this phage can be safely used for biocontrol purposes.
authors: Canadian Research Institute for Food Safety, University of Guelph, ON; N1G 2W1, Canada. hanany@alumni.uoguelph.ca
authors: Hany
authors: H
authors: Anany
authors: Erika J
authors: EJ
authors: Lingohr
authors: Andre
authors: A
authors: Villegas
authors: Hans-Wolfgang
authors: HW
authors: Ackermann
authors: Yi-Min
authors: YM
authors: She
authors: Mansel W
authors: MW
authors: Griffiths
authors: Andrew M
authors: AM
authors: Kropinski
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