title: Effect of interfacial proteins on osteoblast-like cell adhesion to hydroxyapatite nanocrystals.
abstract: A quartz crystal microbalance with dissipation (QCM-D) technique was employed to detecting the protein adsorption and subsequent osteoblast-like cell adhesion to hydroxyapatite (HAp) nanocrystals. The interfacial phenomena with the preadsorption of three proteins (albumin (BSA), fibronectin (Fn), and collagen (Col)), the subsequent adsorption of fetal bovine serum (FBS), and the adhesion of the cells were investigated. The QCM-D measured the frequency shift (Δf) and dissipation energy shift (ΔD), and the viscoelastic properties of the adlayers were evaluated using ΔD-Δf plot and Voigt-based viscoelastic model. The Col adsorption significantly showed higher Δf, ΔD, elasticity, and viscosity values as compared to the BSA and Fn adsorption, and the subsequent FBS adsorption depended on the preadsorbed proteins. The ΔD-Δf plot of the cell adhesion also showed a different behavior depending on the surfaces, and the Fn- and Col-modified surfaces showed the rapid mass and ΔD changes by forming the viscous interfacial layers with cell adhesion, indicating that the processes were affected by the cellular reaction through the extracellular matrix (ECM) proteins. The confocal laser scanning microscope images of adherent cells showed a different morphology and pseudopod on the surfaces. The cells adhered to the surfaces modified with the Fn and Col had significantly uniaxially expanded shapes and fibrous pseudopods, and those modified with the BSA had a round shape. Therefore, the different cell-protein interactions would cause the arrangement of the ECM and the cytoskeleton changes at the interfaces, and these phenomena were successfully detected by the QCM-D and Voigt-based model.
authors: Department of Metallurgy and Ceramics Science, Tokyo Institute of Technology, O-okayama 2-12-1, Tokyo 152-8550, Japan. tagaya.m.aa@m.titech.ac.jp
authors: Motohiro
authors: M
authors: Tagaya
authors: Toshiyuki
authors: T
authors: Ikoma
authors: Taro
authors: T
authors: Takemura
authors: Nobutaka
authors: N
authors: Hanagata
authors: Tomohiko
authors: T
authors: Yoshioka
authors: Junzo
authors: J
authors: Tanaka