title: Basement membrane-like matrix sponge for the three-dimensional proliferation culture of differentiated retinal horizontal interneurons.
abstract: As the neurons in the central nervous system (CNS) form a neuronal network in a three-dimensional (3D) manner, a 3D proliferation culture system for differentiated neurons has been desired. Although differentiated neurons were previously thought to never proliferate, differentiated horizontal interneurons of Rb-/-; p107+/-; p130-/- (p107-single) retina clonally proliferated without dedifferentiation in vivo. In the present study, we developed a basement membrane-like matrix sponge (BM-sponge) for the 3D proliferation culture of differentiated horizontal interneurons. p107-single horizontal interneurons, but not other types of retinal neurons, proliferated in the BM-sponge in a 3D manner. These interneurons expressed presynaptic marker and developed synaptic vesicles. These data demonstrated that p107-single horizontal interneurons cultured in the BM-sponge proliferate while maintaining their differentiated features. We described here the 3D proliferation culture system for differentiated neurons.
authors: Center for Brain Integration Research, Tokyo Medical and Dental University, Tokyo, Japan. iajioka@ams.kuramae.ne.jp
authors: Itsuki
authors: I
authors: Ajioka
authors: Shizuko
authors: S
authors: Ichinose
authors: Kazunori
authors: K
authors: Nakajima
authors: Hidehiro
authors: H
authors: Mizusawa