title: Genome-wide identification of micro-ribonucleic acids associated with human endometrial receptivity in natural and stimulated cycles by deep sequencing.
abstract: OBJECTIVE
  To identify microRNAs (miRNAs) associated with endometrial receptivity.
  
  
  METHODS
  Observational study.
  
  
  METHODS
  Medical center.
  
  
  METHODS
  Healthy, regularly cycling women undergoing IVF treatment.
  
  
  METHODS
  Gonadotropin stimulation and endometrial biopsy.
  
  
  METHODS
  Quantification of miRNA expression profiles by deep sequencing.
  
  
  RESULTS
  The miRNA expression profiles in human endometrium on days LH+2 and LH+7 (LH = 0 is the day of the LH surge) in natural cycles as well as on days hCG+4 and hCG+7 (hCG = 0 is the day of hCG injection) in stimulated cycles were determined by deep sequencing. In natural cycles, there were 20 significantly changed miRNAs in human endometrium on LH+7 compared with LH+2. These miRNAs were predicted to target a large set of genes with different functions, including cell cycle, transport, cell adhesion, cell death, and metabolism. In stimulated cycles, 22 miRNAs were significantly dysregulated on hCG+7 in comparison with LH+7, 11 of which exhibited putative estrogen response elements or P response elements in the promoters. Additionally, unsupervised hierarchical clustering analysis demonstrated that the miRNA expression profile on hCG+4 was similar to that on LH+7, suggesting that ovarian stimulation may alter the window of endometrial receptivity.
  
  
  CONCLUSIONS
  MiRNAs may be novel biomarkers for human endometrial receptivity and may help optimize the protocol for IVF treatment.
authors: Reproductive Medicine Center, Bailu Hospital, Xiamen, People's Republic of China.
authors: Ai-Guo
authors: AG
authors: Sha
authors: Ji-Long
authors: JL
authors: Liu
authors: Xiao-Ming
authors: XM
authors: Jiang
authors: Jian-Zhi
authors: JZ
authors: Ren
authors: Cai-Hui
authors: CH
authors: Ma
authors: Wei
authors: W
authors: Lei
authors: Ren-Wei
authors: RW
authors: Su
authors: Zeng-Ming
authors: ZM
authors: Yang