title: Perfusion method for bone marrow cell collection in poor mobilizer lymphoma patient.
abstract: We previously described a unique procedure for the collection of bone marrow cells (BMCs) using a perfusion method (PM). In cynomolgus monkeys, this method resulted in lower contamination with T cells (<10%). Here, we performed PM on a poor mobilizer lymphoma patient. We confirmed the safety of the intra-bone marrow injection of saline to collect the BMCs. The collected BMCs showed minimal contamination with T cells (<15%) and red blood cells (RBCs) (<4%) from the peripheral blood. It took a total of only 30 min to collect the BMCs. Moreover, transfusion of RBCs was unnecessary. There were no relevant post-operative side effects except for self-limiting pain at the sites of collection, and the patient was able to walk around the hospital after the operation.
authors: The First Department of Internal Medicine, Kansai Medical University, 10-15 Fumizono-cho, Moriguchi, Osaka, 570-8506, Japan.
authors: Shinichiro
authors: S
authors: Mori
authors: The First Department of Internal Medicine, Kansai Medical University, 10-15 Fumizono-cho, Moriguchi, Osaka, 570-8506, Japan.
authors: Shinya
authors: S
authors: Fujita
authors: The First Department of Internal Medicine, Kansai Medical University, 10-15 Fumizono-cho, Moriguchi, Osaka, 570-8506, Japan.
authors: Yoshihisa
authors: Y
authors: Yamamoto
authors: Department of Stem Cell Disorders, Kansai Medical University, 10-15 Fumizono-cho, Moriguchi, Osaka, 570-8506, Japan.
authors: Ming
authors: M
authors: Li
authors: The First Department of Internal Medicine, Kansai Medical University, 10-15 Fumizono-cho, Moriguchi, Osaka, 570-8506, Japan.
authors: Shirou
authors: S
authors: Fukuhara
authors: The First Department of Internal Medicine, Kansai Medical University, 10-15 Fumizono-cho, Moriguchi, Osaka, 570-8506, Japan.
authors: Shosaku
authors: S
authors: Nomura
authors: Department of Stem Cell Disorders, Kansai Medical University, 10-15 Fumizono-cho, Moriguchi, Osaka, 570-8506, Japan. ikehara@takii.kmu.ac.jp.
authors: Susumu
authors: S
authors: Ikehara
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