title: Microglial activation, recruitment and phagocytosis as linked phenomena in ferric oxide nanoparticle exposure.
abstract: Microglia as the resident macrophage-like cells in the central nervous system (CNS) play a pivotal role in the innate immune responses of CNS. Understanding the reactions of microglia cells to nanoparticle exposure is important in the exploration of neurobiology of nanoparticles. Here we provide a systemic mapping of microglia and the corresponding pathological changes in olfactory-transport related brain areas of mice with Fe(2)O(3)-nanoparticle intranasal treatment. We showed that intranasal exposure of Fe(2)O(3) nanoparticle could lead to pathological alteration in olfactory bulb, hippocampus and striatum, and caused microglial proliferation, activation and recruitment in these areas, especially in olfactory bulb. Further experiments with BV2 microglial cells showed the exposure to Fe(2)O(3) nanoparticles could induce cells proliferation, phagocytosis and generation of ROS and NO, but did not cause significant release of inflammatory factors, including IL-1β, IL-6 and TNF-α. Our results indicate that microglial activation may act as an alarm and defense system in the processes of the exogenous nanoparticles invading and storage in brain.
authors: Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety and Key Laboratory of Nuclear Analytical Techniques, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China.
authors: Yun
authors: Y
authors: Wang
authors: Bing
authors: B
authors: Wang
authors: Mo-Tao
authors: MT
authors: Zhu
authors: Ming
authors: M
authors: Li
authors: Hua-Jian
authors: HJ
authors: Wang
authors: Meng
authors: M
authors: Wang
authors: Hong
authors: H
authors: Ouyang
authors: Zhi-Fang
authors: ZF
authors: Chai
authors: Wei-Yue
authors: WY
authors: Feng
authors: Yu-Liang
authors: YL
authors: Zhao