title: Synthesis and application of an N-acylated l-homoserine lactone derivatized affinity matrix for the isolation of quorum sensing signal receptors.
abstract: The design and synthesis of an agarose resin functionalized with a Gram-negative quorum sensing (QS) signaling molecule analogue is described. The modified resin was utilized in affinity pull-down assays to successfully isolate QscR, a LuxR-type QS receptor from Pseudomonas aeruginosa. This resin may facilitate the identification of novel QS signal receptors using affinity chromatography techniques.
authors: Department of Chemistry, University of Wisconsin-Madison, 1101 University Avenue, Madison, WI 53706-1322, USA.
authors: Thanit
authors: T
authors: Praneenararat
authors: Teresa M J
authors: TM
authors: Beary
authors: Anthony S
authors: AS
authors: Breitbach
authors: Helen E
authors: HE
authors: Blackwell
references: Mol Biosyst. 2005 Sep;1(3):196-202
references: 16880983
references: Chem Rev. 2011 Jan 12;111(1):68-85
references: 21125993
references: Nat Protoc. 2010 Feb;5(2):282-93
references: 20134428
references: Chem Biol. 2007 Oct;14(10):1119-27
references: 17961824
references: Chem Commun (Camb). 2009 Dec 21;(47):7378-80
references: 20024234
references: Science. 1996 Apr 19;272(5260):408-11
references: 8602529
references: Chem Soc Rev. 2008 Jul;37(7):1347-60
references: 18568161
references: J Org Chem. 2010 Oct 15;75(20):6737-46
references: 20672805
references: Nature. 1989 Oct 26;341(6244):758-60
references: 2477715
references: Chem Rev. 2011 Jan 12;111(1):195-208
references: 21087050
references: Org Biomol Chem. 2010 Dec 7;8(23):5313-23
references: 20886127
references: J Mol Biol. 2006 Jan 13;355(2):262-73
references: 16307757
references: Chem Biol. 2009 Sep 25;16(9):961-70
references: 19778724
references: J Biol Chem. 2007 May 4;282(18):13592-600
references: 17363368
references: Nat Rev Mol Cell Biol. 2002 Sep;3(9):685-95
references: 12209128
references: Curr Opin Microbiol. 2009 Apr;12(2):205-14
references: 19251475
references: Org Biomol Chem. 2008 Nov 21;6(22):4120-4
references: 18972042
references: Bioorg Med Chem Lett. 2008 May 15;18(10):3072-5
references: 18083553
references: Chem Rev. 2011 Jan 12;111(1):28-67
references: 21182299
references: J Med Chem. 2010 Nov 11;53(21):7467-89
references: 20669927
references: Annu Rev Cell Dev Biol. 2005;21:319-46
references: 16212498
references: Infect Immun. 1998 Jan;66(1):36-42
references: 9423836
references: Proc Natl Acad Sci U S A. 2001 Feb 27;98(5):2752-7
references: 11226312
references: Chem Rev. 2011 Jan 12;111(1):100-16
references: 20536120
references: J Am Chem Soc. 2009 Aug 5;131(30):10610-9
references: 19585989
references: Microbiol Rev. 1979 Dec;43(4):496-518
references: 396467
references: Nat Rev Microbiol. 2004 Jul;2(7):581-92
references: 15197393
references: Science. 2006 Feb 24;311(5764):1113-6
references: 16497924
references: Nature. 2002 Jun 27;417(6892):971-4
references: 12087407
references: J Bacteriol. 1970 Oct;104(1):313-22
references: 5473898
references: Infect Immun. 2000 Sep;68(9):4839-49
references: 10948095
references: EMBO J. 2002 Sep 2;21(17):4393-401
references: 12198141
references: FEMS Microbiol Rev. 2010 Mar;34(2):150-70
references: 20070373
references: Chem Commun (Camb). 2009 Dec 14;(46):7107-9
references: 19920998
references: Trends Microbiol. 2010 Sep;18(9):383-7
references: 20573513
references: Chem Soc Rev. 2008 Jul;37(7):1432-47
references: 18568169
references: J Bacteriol. 2011 Jan;193(2):421-8
references: 21097632
references: Chem Biol. 2009 Sep 25;16(9):913-4
references: 19778715
references: Mol Microbiol. 2006 Jan;59(2):602-9
references: 16390453
references: Methods Enzymol. 1999;310:117-28
references: 10547786
references: J Am Chem Soc. 2006 Mar 8;128(9):2802-3
references: 16506750