title: A putative octopamine/tyramine receptor mediating appetite in a hungry fly.
abstract: In the blowfly Phormia regina, experience of simultaneous feeding with D: -limonene exposure inhibits proboscis extension reflex (PER) due to decreased tyramine (TA) titer in the brain. To elucidate the molecular mechanism of TA signaling pathway related to the associated feeding behavior, we cloned cDNA encoding the octopamine/TA receptor (PregOAR/TAR). The deduced protein is composed of 607 amino acid residues and has 7 predicted transmembrane domains. Based on homology and phylogenetic analyses, this protein belongs to the OAR/TAR family. The PregOAR/TAR was mainly expressed in head, with low levels of expression in other tissues at adult stages. Gene expression profile is in agreement with a plethora of functions ascribed to TA in various insect tissues. The immunolabeled cell bodies and processes were localized in the medial protocerebrum, outer layer of lobula, antennal lobe, and subesophageal ganglion. These results suggest that decrease of TA level in the brain likely affects neurons expressing PregOAR/TAR, causing mediation of the sensitivity in the sensillum and/or output of motor neurons for PER.
authors: Department of Biology, Graduate School of Science, Kobe University, Rokkodai-cho, Nada-ku, Kobe, Japan, yishida@eagle.kobe-u.ac.jp
authors: Yuko
authors: Y
authors: Ishida
authors: Mamiko
authors: M
authors: Ozaki
references: Insect Mol Biol. 2010 Aug;19(4):489-99
references: 20491982
references: J Insect Physiol. 1998 Mar;44(3-4):333-342
references: 12769967
references: Annu Rev Entomol. 2005;50:447-77
references: 15355245
references: Neuron. 1990 Mar;4(3):343-54
references: 2156539
references: Mol Biol Evol. 2007 Aug;24(8):1596-9
references: 17488738
references: Insect Biochem Mol Biol. 2002 Dec;32(12):1775-80
references: 12429129
references: Insect Mol Biol. 2003 Jun;12(3):217-23
references: 12752654
references: Gen Comp Endocrinol. 2009 May 15;162(1):18-26
references: 18588893
references: Cell Tissue Res. 2009 Feb;335(2):455-63
references: 19034524
references: EMBO J. 1990 Nov;9(11):3611-7
references: 2170118
references: J Neurochem. 2000 Mar;74(3):900-8
references: 10693920
references: Neuroscience. 2009 Sep 15;162(4):1120-33
references: 19482069
references: J Neurosci. 2005 Aug 17;25(33):7507-16
references: 16107638