title: VEGF gene polymorphism association with diabetic foot ulcer.
abstract: OBJECTIVE
  Functional polymorphisms within vascular endothelial growth factor (VEGF) gene have shown association with various conditions including diabetic neuropathy and retinopathy. In this study we have performed a candidate gene association study in order to examine VEGF gene polymorphism association with diabetic foot ulcer (DFU).
  
  
  METHODS
  The study group comprised of type 2 diabetes patients with (N=247) and without (N=241) DFU. Healthy control subjects (N=98) were also recruited from the same area. The ARMS-PCR technique was applied for genotyping of VEGF gene SNPs at positions -7*C/T and -2578*C/A.
  
  
  RESULTS
  The frequency of genotype AA was significantly decreased in patients with DFU compared with diabetic subjects without DFU (AA vs CA+CC, p=0.003, OR=0.44, CI=0.24-0.80). Also there was a significant decrease in frequency of A allele in patients with DFU compared to the controls (p=0.02, OR=0.68, CI=0.48-0.96).
  
  
  CONCLUSIONS
  It seems that lower frequency of A allele in patients with DFU is conferring a protective effect which might be as a result of increased angiogenesis in patients carrying this allele.
authors: EMRC (Endocrinology and Metabolism Research Center), Tehran University of Medical Sciences, Tehran, Iran. Electronic address: amolimm@tums.ac.ir.
authors: Mahsa M
authors: MM
authors: Amoli
authors: EMRC (Endocrinology and Metabolism Research Center), Tehran University of Medical Sciences, Tehran, Iran.
authors: Shirin
authors: S
authors: Hasani-Ranjbar
authors: EMRC (Endocrinology and Metabolism Research Center), Tehran University of Medical Sciences, Tehran, Iran.
authors: Nahid
authors: N
authors: Roohipour
authors: EMRC (Endocrinology and Metabolism Research Center), Tehran University of Medical Sciences, Tehran, Iran.
authors: Forough A
authors: FA
authors: Sayahpour
authors: EMRC (Endocrinology and Metabolism Research Center), Tehran University of Medical Sciences, Tehran, Iran.
authors: Parvin
authors: P
authors: Amiri
authors: EMRC (Endocrinology and Metabolism Research Center), Tehran University of Medical Sciences, Tehran, Iran.
authors: Parisa
authors: P
authors: Zahedi
authors: Science and Research Branch, Islamic Azad university, Tehran, Iran.
authors: Mahdie
authors: M
authors: Mehrab-Mohseni
authors: EMRC (Endocrinology and Metabolism Research Center), Tehran University of Medical Sciences, Tehran, Iran.
authors: Ramin
authors: R
authors: Heshmat
authors: EMRC (Endocrinology and Metabolism Research Center), Tehran University of Medical Sciences, Tehran, Iran.
authors: Bagher
authors: B
authors: Larijani
authors: Department of Medical Genetics, School of Medicine, Tehran university of Medical Sciences, Poursina St., Tehran 14176, Iran. Electronic address: tavakkolybazzazj@tums.ac.ir.
authors: Javad
authors: J
authors: Tavakkoly-Bazzaz