title: Rapid resolution liquid chromatography (RRLC) analysis for quality control of Rhodiola rosea roots and commercial standardized products.
abstract: A simple, sensitive and reliable reversed phase Rapid Resolution Liquid Chromatography (RRLC) method was developed and validated for six biologically active compounds (salidroside, tyrosol, rosarin, rosavin, rosin and rosiridin) in Rhodiola rosea L. roots and powder extracts. The method uses a Phenomenex C18 (2)-HST column at 40 degrees C with a neutral gradient system mobile phase (H20 and acetonitrile), a flow rate of 1.0 mL/min, and UV detection wavelengths set at 205 and 254 nm, simultaneously. Baseline separation of the six active compounds was achieved within 8 minutes. The average percentages of rosavins (rosarin, rosavin, and rosin) in authentic R. rosea roots and root powder extracts were quantitatively determined and a characteristic R. rosea roots RRLC profile was established. The RRLC method is accurate and sensitive; in addition, it effectively increases the sample analysis throughput compared with conventional HPLC.
authors: Canadian Phytopharmaceuticals Corp., 12233 Riverside Way, Richmond, B.C. Canada V6W 1K8.
authors: Yuan-Chun
authors: YC
authors: Ma
authors: Xiao-Qiang
authors: XQ
authors: Wang
authors: Fei Fei
authors: FF
authors: Hou
authors: Jie
authors: J
authors: Ma
authors: Mai
authors: M
authors: Luo
authors: Shane
authors: S
authors: Lu
authors: Peter
authors: P
authors: Jin
authors: Nelly
authors: N
authors: Terevsky
authors: Alice
authors: A
authors: Chen
authors: Iris
authors: I
authors: Xu
authors: Asmita V
authors: AV
authors: Patel
authors: Dariusz
authors: D
authors: Gorecki