title: Antibody covalent immobilization on carbon nanotubes and assessment of antigen binding.
abstract: Controlling the covalent bonding of antibodies onto functionalized carbon nanotubes is a key step in the design and preparation of nanotube-based conjugates for targeting cancer cells. For this purpose, an anti-MUC1 antibody (Ab) is linked to both multi-walled (MWCNTs) and double-walled carbon nanotubes (DWCNTs) using different synthetic strategies. The presence of the Ab attached to the nanotubes is confirmed by gel electrophoresis and thermogravimetric analysis. Most importantly, molecular recognition of the antigen by surface plasmon resonance is able to determine similar Ab binding capacities for both Ab-DWCNTs and Ab-MWCNTs. These results are very relevant for the design of future receptor-targeting strategies using chemically functionalized carbon nanotubes.
authors: CNRS, Institut de Biologie Moléculaire et Cellulaire, Laboratoire d'Immunologie et Chimie Thérapeutiques, Strasbourg 67000, France.
authors: Enrica
authors: E
authors: Venturelli
authors: Chiara
authors: C
authors: Fabbro
authors: Olivier
authors: O
authors: Chaloin
authors: Cécilia
authors: C
authors: Ménard-Moyon
authors: Cristian R
authors: CR
authors: Smulski
authors: Tatiana
authors: T
authors: Da Ros
authors: Kostas
authors: K
authors: Kostarelos
authors: Maurizio
authors: M
authors: Prato
authors: Alberto
authors: A
authors: Bianco