title: Manufacturing of adeno-associated viruses, for example: AAV2.
abstract: Adeno-associated virus (AAV) is one of the most promising vectors for gene therapy. There are several ways of producing AAV vectors but large-scale production of this vector remains a major challenge. Virovek developed a novel method of expressing the AAV Rep and Cap genes in insect cells mediated by intron-splicing mechanism and producing AAV vectors with these Rep and Cap sequences containing the artificial intron. The recombinant baculoviruses harboring these artificial intron-containing Rep and Cap sequences are very stable and the AAV vectors produced in insect cells with these recombinant baculoviruses are very infectious.
authors: Virovek, Inc., San Francisco, CA, USA. haifengchen05@yahoo.com
authors: Haifeng
authors: H
authors: Chen