title: Altered binding of the ap-1 protein on a negative regulatory element of C-myc is correlated with the progression of the malignancy of the lung and may contribute to C-myc expression.
abstract: The binding activity of the AP-1 (FOS/JUN) complex to a negative regulatory element on the c-myc promoter was investigated in 7 tumour- versus normal lung specimens and it correlated with c-myc expression. AP-1 levels were found elevated in two samples representing stage I progression of the disease, while in three stage III tumour samples, the AP-1 binding activity was equal or slightly elevated compared with their normal adjacent tissue. Southern blot analysis revealed amplified c-myc in all three cases of stage III tumours. These results suggest that there is a negative correlation between FOS/JUN binding on c-myc promoter and the expression of c-myc during the progression of the disease, further supporting the fundamental role of AP-1 on c-myc regulation.
authors: NATL HELLEN RES FND,INST BIOL RES & BIOTECHNOL,GR-11635 ATHENS,GREECE. UNIV CRETE,SCH MED,IRAKLION,GREECE.
authors: A
authors: A
authors: Eliopoulos
authors: D
authors: D
authors: Spandidos