title: In vitro reconstitution of eukaryotic ion channels using droplet interface bilayers.
abstract: The ability to routinely study eukaryotic ion channels in a synthetic lipid environment would have a major impact on our understanding of how different lipids influence ion channel function. Here, we describe a straightforward, detergent-free method for the in vitro reconstitution of eukaryotic ion channels and ionotropic receptors into droplet interface bilayers and measure their electrical activity at both the macroscopic and single-channel level. We explore the general applicability of this method by reconstitution of channels from a wide range of sources including recombinant cell lines and native tissues, as well as preparations that are difficult to study by conventional methods including erythrocytes and mitochondria.
authors: Department of Chemistry, University of Oxford, 12 Mansfield Road, Oxford OX1 3TA, United Kingdom.
authors: Sebastian
authors: S
authors: Leptihn
authors: James R
authors: JR
authors: Thompson
authors: J Clive
authors: JC
authors: Ellory
authors: Stephen J
authors: SJ
authors: Tucker
authors: Mark I
authors: MI
authors: Wallace