title: High-throughput analysis of single hematopoietic stem cell proliferation in microfluidic cell culture arrays.
abstract: Heterogeneity in cell populations poses a major obstacle to understanding complex biological processes. Here we present a microfluidic platform containing thousands of nanoliter-scale chambers suitable for live-cell imaging studies of clonal cultures of nonadherent cells with precise control of the conditions, capabilities for in situ immunostaining and recovery of viable cells. We show that this platform mimics conventional cultures in reproducing the responses of various types of primitive mouse hematopoietic cells with retention of their functional properties, as demonstrated by subsequent in vitro and in vivo (transplantation) assays of recovered cells. The automated medium exchange of this system made it possible to define when Steel factor stimulation is first required by adult hematopoietic stem cells in vitro as the point of exit from quiescence. This technology will offer many new avenues to interrogate otherwise inaccessible mechanisms governing mammalian cell growth and fate decisions.
authors: Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada.
authors: Véronique
authors: V
authors: Lecault
authors: Michael
authors: M
authors: Vaninsberghe
authors: Sanja
authors: S
authors: Sekulovic
authors: David J H F
authors: DJ
authors: Knapp
authors: Stefan
authors: S
authors: Wohrer
authors: William
authors: W
authors: Bowden
authors: Francis
authors: F
authors: Viel
authors: Thomas
authors: T
authors: McLaughlin
authors: Asefeh
authors: A
authors: Jarandehei
authors: Michelle
authors: M
authors: Miller
authors: Didier
authors: D
authors: Falconnet
authors: Adam K
authors: AK
authors: White
authors: David G
authors: DG
authors: Kent
authors: Michael R
authors: MR
authors: Copley
authors: Fariborz
authors: F
authors: Taghipour
authors: Connie J
authors: CJ
authors: Eaves
authors: R Keith
authors: RK
authors: Humphries
authors: James M
authors: JM
authors: Piret
authors: Carl L
authors: CL
authors: Hansen
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