title: Sumoylation of CCAAT/enhancer-binding protein α promotes the biased primitive hematopoiesis of zebrafish.
abstract: Hematopoiesis is evolutionarily conserved from zebrafish to mammals, and this includes both primitive and definitive waves during embryogenesis. Primitive hematopoiesis is dominated by erythropoiesis with limited myelopoiesis. Protein sumoylation, a ubiquitination-like posttranslational protein modification, is implicated in a variety of biochemical processes, most notably in transcriptional repression. We show here that the loss of 6 small ubiquitin-related modifier (SUMO) paralogs triggers a sharp up-regulation of the myeloid-specific marker mpo and down-regulation of the erythroid-specific marker gata1 in myelo-erythroid progenitor cells (MPCs) in the intermediate cell mass (ICM) during primitive hematopoiesis. Accordingly, in transgenic zebrafish lines, hyposumoylation expands myelopoiesis at the expense of erythropoiesis. A SUMO-CCAAT/enhancer-binding protein α (SUMO-C/ebpα) fusion restores the normal myelopoiesis/erythropoiesis balance, suggesting that sumoylation status of C/ebpα contributes to myelo-erythroid lineage determination. Our results therefore implicate sumoylation in early lineage determination and reveal the possible molecular mechanism underlying the puzzling biased primitive hematopoiesis in vertebrates.
authors: Centre National de la Recherche Scientifique-LIA124, Sino-French Research Center for Life Sciences and Genomics, Shanghai Institute of Hematology, Rui-Jin Hospital, China.
authors: Hao
authors: H
authors: Yuan
authors: Jun
authors: J
authors: Zhou
authors: Min
authors: M
authors: Deng
authors: Yong
authors: Y
authors: Zhang
authors: Yi
authors: Y
authors: Chen
authors: Yi
authors: Y
authors: Jin
authors: Jiang
authors: J
authors: Zhu
authors: Sai Juan
authors: SJ
authors: Chen
authors: Hugues
authors: H
authors: de The
authors: Zhu
authors: Z
authors: Chen
authors: Ting Xi
authors: TX
authors: Liu
authors: Jun
authors: J
authors: Zhu