title: The type III secretion system is involved in Escherichia coli K1 interactions with Acanthamoeba.
abstract: The type III secretion system among Gram-negative bacteria is known to deliver effectors into host cell to interfere with host cellular processes. The type III secretion system in Yersina, Pseudomonas and Enterohemorrhagic Escherichia coli have been well documented to be involved in the bacterial pathogenicity. The existence of type III secretion system has been demonstrated in neuropathogenic E. coli K1 strains. Here, it is observed that the deletion mutant of type III secretion system in E. coli strain EC10 exhibited defects in the invasion and intracellular survival in Acanthamoeba castellanii (a keratitis isolate) compared to its parent strain. Next, it was determined whether type III secretion system plays a role in E. coli K1 survival inside Acanthamoeba during the encystment process. Using encystment assays, our findings revealed that the type III secretion system-deletion mutant exhibited significantly reduced survival inside Acanthamoeba cysts compared with its parent strain, EC10 (P<0.01). This is the first demonstration that the type III secretion system plays an important role in E. coli interactions with Acanthamoeba. A complete understanding of how amoebae harbor bacterial pathogens will help design strategies against E. coli transmission to the susceptible hosts.
authors: Aga Khan University, Stadium Road, Karachi, Pakistan.
authors: Ruqaiyyah
authors: R
authors: Siddiqui
authors: Huma
authors: H
authors: Malik
authors: Mehwish
authors: M
authors: Sagheer
authors: Suk-Yul
authors: SY
authors: Jung
authors: Naveed Ahmed
authors: NA
authors: Khan