title: MicroRNA expression in the livers of inbred mice.
abstract: MicroRNAs are short, non-coding RNA sequences that regulate genes at the post-transcriptional level and have been shown to be important in development, tissue differentiation, and disease. Limited attention has been given to the natural variation in miRNA expression across genetically diverse populations even though it is well established that genetic polymorphisms can have a profound effect on mRNA levels. Expression level of 577 miRNAs in the livers of 70 strains of inbred mice was assessed, and we found that miRNA expression is highly stable across different strains. Globally, the expression of miRNA target transcripts does not correlate with miRNA expression, primarily due to the low variance of miRNA but high variance of mRNA expression across strains. Our results show that there is little genetic effect on the baseline miRNA levels in murine liver. The stability of mouse liver miRNA expression in a genetically diverse population suggests that treatment-induced disruptions in liver miRNA expression, a phenomenon established for a large number of toxicants, may indicate an important mechanism for the disturbance of normal liver function, and may prove to be a useful genetic background-independent biomarker of toxicant effect.
authors: Department of Environmental Sciences & Engineering, University of North Carolina, Chapel Hill, NC 27599, USA.
authors: Daniel M
authors: DM
authors: Gatti
authors: Lu
authors: L
authors: Lu
authors: Robert W
authors: RW
authors: Williams
authors: Wei
authors: W
authors: Sun
authors: Fred A
authors: FA
authors: Wright
authors: David W
authors: DW
authors: Threadgill
authors: Ivan
authors: I
authors: Rusyn
references: Lab Invest. 2010 Oct;90(10):1437-46
references: 20548288
references: Genome Res. 2009 Jan;19(1):92-105
references: 18955434
references: Gastroenterology. 2009 Mar;136(3):1081-90
references: 19185580
references: FEBS Lett. 2005 Oct 31;579(26):5822-9
references: 16214139
references: Hum Mol Genet. 2009 Dec 15;18(24):4818-29
references: 19776030
references: J Toxicol Sci. 2007 Oct;32(4):401-9
references: 17965554
references: Hepatology. 2007 Aug;46(2):548-57
references: 17542012
references: Mol Cell. 2007 Jun 8;26(5):745-52
references: 17540599
references: Nucleic Acids Res. 2008 Jan;36(Database issue):D154-8
references: 17991681
references: Differentiation. 2009 Feb;77(2):181-7
references: 19281778
references: PLoS One. 2010 Feb 01;5(2):e8898
references: 20126538
references: Genome Biol. 2008;9(5):R82
references: 18485210
references: Nat Methods. 2004 Oct;1(1):47-53
references: 15782152
references: Bioinformatics. 2009 Feb 15;25(4):482-9
references: 19091771
references: Toxicol Appl Pharmacol. 2009 Mar 15;235(3):305-11
references: 19167416
references: Stat Appl Genet Mol Biol. 2008;7(1):Article22
references: 18673291
references: Nat Genet. 2005 May;37(5):495-500
references: 15806104
references: Bioinformatics. 2009 Dec 1;25(23):3049-55
references: 19789267
references: Mamm Genome. 2009 Jul;20(7):437-46
references: 19609828
references: Cold Spring Harb Symp Quant Biol. 2006;71:523-30
references: 17381335
references: Nature. 2005 Feb 17;433(7027):769-73
references: 15685193
references: RNA. 2005 Sep;11(9):1461-70
references: 16043497
references: Mol Cell Biol. 2007 Jun;27(12):4238-47
references: 17438130
references: Nucleic Acids Res. 2008 Jan;36(Database issue):D149-53
references: 18158296
references: Int J Cancer. 2010 Oct 15;127(8):1785-94
references: 20099276
references: Hepatology. 2009 Feb;49(2):618-26
references: 19127519
references: RNA. 2004 Nov;10(11):1813-9
references: 15496526
references: PLoS Comput Biol. 2009 Sep;5(9):e1000513
references: 19779543
references: Nucleic Acids Res. 2006 Mar 31;34(6):1765-71
references: 16582102
references: RNA. 2005 Mar;11(3):241-7
references: 15701730
references: Mol Cell. 2006 Feb 17;21(4):533-42
references: 16483934
references: Methods Mol Biol. 2009;541:225-45
references: 19381545
references: Nucleic Acids Res. 2006 Mar 20;34(5):1646-52
references: 16549876
references: PLoS Biol. 2004 Dec;2(12):e393
references: 15534693
references: Mamm Genome. 2009 Aug;20(8):476-85
references: 19727952
references: J Hum Genet. 2008;53(6):515
references: 18465083
references: Gastroenterology. 2009 Jun;136(7):2304-2315.e1-4
references: 19272382
references: Genome Res. 2009 Mar;19(3):481-90
references: 19088304
references: Toxicology. 2008 Apr 3;246(1):34-9
references: 18289762
references: PLoS One. 2009 Jun 11;4(6):e5878
references: 19517021
references: Proc Natl Acad Sci U S A. 2003 Aug 5;100(16):9440-5
references: 12883005
references: Nat Methods. 2007 Dec;4(12):1045-9
references: 18026111
references: Nat Genet. 2003 Nov;35(3):215-7
references: 14528307
references: Cell. 2004 Jan 23;116(2):281-97
references: 14744438
references: Genome Res. 2004 Dec;14(12):2486-94
references: 15574827
references: Nature. 2005 Mar 17;434(7031):338-45
references: 15735639
references: RNA. 2008 Jan;14(1):35-42
references: 18025253
references: Toxicol Sci. 2009 Jul;110(1):235-43
references: 19420014
references: Nucleic Acids Res. 2007;35(7):e52
references: 17355992
references: Mol Carcinog. 2009 Jun;48(6):479-87
references: 18942116
references: J Hepatol. 2008 Apr;48(4):648-56
references: 18291553