title: Glycosyltransferase structural biology and its role in the design of catalysts for glycosylation.
abstract: Glycosyltransferases (GTs) are ubiquitous in nature and are required for the transfer of sugars to a variety of important biomolecules. This essential enzyme family has been a focus of attention from both the perspective of a potential drug target and a catalyst for the development of vaccines, biopharmaceuticals and small molecule therapeutics. This review attempts to consolidate the emerging lessons from Leloir (nucleotide-dependent) GT structural biology studies and recent applications of these fundamentals toward rational engineering of glycosylation catalysts.
authors: Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA.
authors: Aram
authors: A
authors: Chang
authors: Shanteri
authors: S
authors: Singh
authors: George N
authors: GN
authors: Phillips
authors: Jon S
authors: JS
authors: Thorson
references: Plant Cell. 2010 Aug;22(8):2856-71
references: 20693356
references: EMBO J. 2008 Jan 9;27(1):234-43
references: 18046457
references: Proc Natl Acad Sci U S A. 2007 Mar 27;104(13):5336-41
references: 17376874
references: Glycobiology. 2011 May;21(5):547-52
references: 21098513
references: J Mol Biol. 2007 Sep 7;372(1):67-76
references: 17640665
references: Nat Chem Biol. 2010 May;6(5):321-3
references: 20364127
references: Curr Opin Chem Biol. 2010 Apr;14(2):168-73
references: 20129812
references: Plant Cell. 2009 May;21(5):1556-72
references: 19454730
references: Phytochemistry. 2009 Oct-Nov;70(15-16):1812-21
references: 19559449
references: J Biol Chem. 2010 Feb 12;285(7):4941-50
references: 20007322
references: EMBO J. 2006 Mar 22;25(6):1396-405
references: 16482224
references: J Mol Biol. 2010 Feb 19;396(2):321-31
references: 19941871
references: Phytochemistry. 2009 Mar;70(4):473-82
references: 19261311
references: J Biol Chem. 2006 Nov 10;281(45):34441-7
references: 16982612
references: Proc Natl Acad Sci U S A. 2005 Jan 18;102(3):571-6
references: 15598734
references: J Mol Biol. 2010 Sep 17;402(2):399-411
references: 20655926
references: Angew Chem Int Ed Engl. 2010 Feb 8;49(7):1234-7
references: 20077550
references: J Biol Chem. 2006 Feb 10;281(6):3625-32
references: 16326711
references: Chem Biol. 2008 Apr;15(4):393-401
references: 18420146
references: J Mol Biol. 2007 Jun 1;369(2):498-511
references: 17442341
references: Proc Natl Acad Sci U S A. 2007 Dec 18;104(51):20238-43
references: 18077347
references: Nucleic Acids Res. 2009 Jan;37(Database issue):D233-8
references: 18838391
references: FEBS Lett. 2009 Jun 18;583(12):2131-5
references: 19500551
references: J Biol Chem. 2008 Jun 6;283(23):15834-44
references: 18390549
references: Nature. 2011 Jan 27;469(7331):564-7
references: 21240259
references: Methods Enzymol. 2009;458:309-33
references: 19374988
references: Chem Biol. 2009 Jan 30;16(1):28-35
references: 19171303
references: J Mol Biol. 2009 Oct 9;392(5):1292-302
references: 19683002
references: J Biol Chem. 2004 Sep 10;279(37):38683-92
references: 15148316
references: J Am Chem Soc. 2005 Jul 6;127(26):9336-7
references: 15984838
references: J Biol Chem. 2007 Jul 13;282(28):20705-14
references: 17510062
references: ACS Chem Biol. 2011 Jan 21;6(1):95-100
references: 20886903
references: Phytochemistry. 2009 Feb;70(3):325-47
references: 19217634
references: J Biol Chem. 2004 Jul 2;279(27):28339-44
references: 15075344
references: J Bacteriol. 2009 Apr;191(8):2871-5
references: 19233921
references: Chem Biol. 2002 Mar;9(3):287-95
references: 11927254
references: J Biol Chem. 2009 Jul 3;284(27):17926-34
references: 19411659
references: Curr Drug Targets. 2008 Apr;9(4):292-309
references: 18393823
references: Nature. 2007 Apr 26;446(7139):1008-16
references: 17460661
references: Drug Metab Rev. 2010 Feb;42(1):159-66
references: 19807219
references: FEBS Lett. 2011 Feb 18;585(4):645-50
references: 21251913
references: Nat Chem Biol. 2011 Feb;7(2):78-80
references: 21196935
references: Plant Physiol. 2008 Nov;148(3):1295-308
references: 18829982
references: Annu Rev Biochem. 2008;77:521-55
references: 18518825
references: J Mol Biol. 2010 Mar 26;397(2):436-47
references: 20097205
references: Nat Struct Mol Biol. 2009 Nov;16(11):1186-8
references: 19820709
references: Chem Biol. 2009 Jun 26;16(6):676-85
references: 19549605
references: Adv Enzymol Relat Areas Mol Biol. 2009;76:55-119
references: 18990828
references: Angew Chem Int Ed Engl. 2008;47(46):8889-92
references: 18924204
references: J Biol Chem. 2010 May 14;285(20):15619-15626
references: 20236943
references: Nat Struct Biol. 2001 Feb;8(2):166-75
references: 11175908
references: Mol Biosyst. 2010 Oct;6(10):1773-81
references: 20556308
references: Biotechnol Bioeng. 2009 Mar 1;102(4):988-94
references: 18985617
references: Biochemistry. 2009 Oct 27;48(42):10089-97
references: 19761218
references: EMBO J. 2000 Oct 16;19(20):5269-80
references: 11032794
references: J Biol Chem. 2011 Apr 29;286(17):15155-64
references: 21288903
references: Nat Struct Mol Biol. 2010 May;17(5):582-9
references: 20400947
references: Nat Chem Biol. 2007 Oct;3(10):657-62
references: 17828251
references: Proc Natl Acad Sci U S A. 2010 Oct 12;107(41):17563-8
references: 20876143
references: Proc Natl Acad Sci U S A. 2003 Aug 5;100(16):9238-43
references: 12874381
references: Chem Biol. 2001 Jun;8(6):557-67
references: 11410375
references: J Biol Chem. 2010 Nov 26;285(48):37741-52
references: 20843801
references: Biochem Biophys Res Commun. 2009 Jan 2;378(1):10-4
references: 18771653
references: Biochemistry. 2004 May 11;43(18):5170-80
references: 15122882