title: Leaf rolling controlled by the homeodomain leucine zipper class IV gene Roc5 in rice.
abstract: Leaf rolling is considered an important agronomic trait in rice (Oryza sativa) breeding. To understand the molecular mechanism controlling leaf rolling, we screened a rice T-DNA insertion population and isolated the outcurved leaf1 (oul1) mutant showing abaxial leaf rolling. The phenotypes were caused by knockout of Rice outermost cell-specific gene5 (Roc5), an ortholog of the Arabidopsis (Arabidopsis thaliana) homeodomain leucine zipper class IV gene GLABRA2. Interestingly, overexpression of Roc5 led to adaxially rolled leaves, whereas cosuppression of Roc5 resulted in abaxial leaf rolling. Bulliform cell number and size increased in oul1 and Roc5 cosuppression plants but were reduced in Roc5-overexpressing lines. The data indicate that Roc5 negatively regulates bulliform cell fate and development. Gene expression profiling, quantitative polymerase chain reaction, and RNA interference (RNAi) analyses revealed that Protodermal Factor Like (PFL) was probably down-regulated in oul1. The mRNA level of PFL was increased in Roc5-overexpressing lines, and PFL-RNAi transgenic plants exhibit reversely rolling leaves by reason of increases of bulliform cell number and size, indicating that Roc5 may have a conserved function. These are, to our knowledge, the first functional data for a gene encoding a homeodomain leucine zipper class IV transcriptional factor in rice that modulates leaf rolling.
authors: Biotechnology Research Institute/National Key Facility for Genetic Resources and Gene Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, People's Republic of China.
authors: Liang-ping
authors: LP
authors: Zou
authors: Xue-hui
authors: XH
authors: Sun
authors: Zhi-guo
authors: ZG
authors: Zhang
authors: Peng
authors: P
authors: Liu
authors: Jin-xia
authors: JX
authors: Wu
authors: Cai-juan
authors: CJ
authors: Tian
authors: Jin-long
authors: JL
authors: Qiu
authors: Tie-gang
authors: TG
authors: Lu
references: Plant J. 2002 Feb;29(4):497-507
references: 11846882
references: Science. 1992 Dec 11;258(5089):1773-5
references: 1465611
references: Development. 2003 Feb;130(4):635-43
references: 12505995
references: Plant J. 2009 Sep;59(6):883-94
references: 19453441
references: Plant Mol Biol. 2008 Oct;68(3):239-50
references: 18594992
references: Plant Mol Biol. 1999 May;40(2):343-54
references: 10412912
references: Dev Biol. 2009 Oct 15;334(2):345-54
references: 19665012
references: J Exp Bot. 2003 May;54(386):1343-50
references: 12709480
references: Plant Cell. 1999 Jul;11(7):1217-26
references: 10402424
references: Plant J. 2001 Jun;26(5):487-94
references: 11439135
references: Plant Cell. 2004 Sep;16(9):2258-63
references: 15342905
references: Stat Appl Genet Mol Biol. 2004;3:Article3
references: 16646809
references: Plant Cell. 2009 Mar;21(3):719-35
references: 19304938
references: Plant Signal Behav. 2009 Feb;4(2):86-8
references: 19649178
references: J Exp Bot. 2000 Mar;51(344):497-505
references: 10938806
references: Plant J. 1996 Sep;10(3):393-402
references: 8811855
references: Biomaterials. 2009 Feb;30(6):991-1004
references: 19046765
references: Genesis. 2001 Aug;30(4):220-30
references: 11536428
references: Plant J. 2000 Jun;22(5):401-14
references: 10849356
references: Biostatistics. 2003 Apr;4(2):249-64
references: 12925520
references: Physiol Plant. 2008 Sep;134(1):174-82
references: 18507789
references: Plant Cell. 1996 Dec;8(12):2155-68
references: 8989876
references: Nucleic Acids Res. 2003 Feb 15;31(4):e15
references: 12582260
references: Development. 1996 Apr;122(4):1253-60
references: 8620852
references: Genes Dev. 1994 Jun 15;8(12):1388-99
references: 7926739
references: Curr Opin Plant Biol. 2007 Feb;10(1):70-82
references: 17140837
references: Plant Physiol. 2007 May;144(1):121-33
references: 17369428
references: Plant Cell. 1990 Apr;2(4):291-9
references: 2152117
references: Development. 1998 Apr;125(7):1161-71
references: 9477315
references: Plant Mol Biol. 2009 Jan;69(1-2):69-80
references: 18830797
references: Mol Plant. 2010 Sep;3(5):807-17
references: 20494951
references: Dev Biol. 1999 Dec 15;216(2):646-58
references: 10642799
references: Sci China C Life Sci. 2009 Sep;52(9):885-90
references: 19802748
references: Plant J. 2002 Nov;32(4):495-508
references: 12445121
references: Plant J. 2009 Nov;60(3):564-74
references: 19619157
references: Proc Natl Acad Sci U S A. 2002 Apr 16;99(8):5460-5
references: 11929961
references: Development. 2002 Nov;129(22):5217-25
references: 12399313
references: Methods. 2001 Dec;25(4):402-8
references: 11846609
references: Planta. 2007 Jun;226(1):99-108
references: 17216479
references: Plant Cell. 1990 Apr;2(4):279-289
references: 12354959
references: Science. 2003 May 30;300(5624):1427-30
references: 12775839
references: Genome. 2007 Sep;50(9):811-7
references: 17893721
references: Plant Mol Biol. 2005 May;58(1):123-39
references: 16028121
references: Appl Opt. 1996 Nov 20;35(33):6585-98
references: 21127682
references: Plant J. 2002 Feb;29(3):359-69
references: 11844112
references: Annu Rev Plant Biol. 2008;59:365-86
references: 18257710
references: Bioinformatics. 2003 Jan 22;19(2):185-93
references: 12538238
references: Plant Cell. 2007 Mar;19(3):847-61
references: 17400897
references: Mol Cell. 2000 Oct;6(4):791-802
references: 11090618