title: Analysis of the proliferative and phenotypic properties of tumor infiltrating lymphocytes expanded in vitro in the course of the clinical trial of adoptive immunotherapy of metastatic melanoma.
abstract: Adoptive immunotherapy with in vitro expanded tumor infiltrating lymphocytes (TIL) and recombinant interleukin-2 (rIL-2) is a recent option in the treatment of advanced melanoma resistant to conventional chemotherapy. In the course of a protocol of treatment of advanced melanoma with in vitro expanded TIL and rIL-2, we obtained 38 samples from 27 different patients. Lymphocytes derived were cultured in the presence of rIL-2 in vitro for a 4-6 week period and 23 resulted in proliferative cultures. Eighteen samples were infused in the course of the clinical trial. The median number of lymphocytes obtained was 18x10(9) (range 1-43x10(9)) cells. Phenotypic analysis showed that all samples contained a pure population of T cells. These data confirm that lymphocytes can be expanded from advanced metastatic sites, have peculiar characteristics and are suitable to be infused in vivo.
authors: IST NAZL RIC CANC,DIV ONCOL CHIRURG,I-16132 GENOA,ITALY. UNIV GENOA,IST ONCOL CLIN,GENOA,ITALY. IST NAZL RIC CANC,SERV PATOL CLIN,I-16132 GENOA,ITALY.
authors: G
authors: G
authors: Pietra
authors: C
authors: C
authors: Semino
authors: S
authors: S
authors: Basso
authors: M
authors: M
authors: Gipponi
authors: G
authors: G
authors: Disomma
authors: A
authors: A
authors: Peressini
authors: F
authors: F
authors: Cafiero
authors: M
authors: M
authors: Ponte
authors: P
authors: P
authors: Queirolo
authors: I
authors: I
authors: Ribizzi
authors: M
authors: M
authors: Sertoli
authors: G
authors: G
authors: Melioli