title: Barley intake induces bile acid excretion by reduced expression of intestinal ASBT and NPC1L1 in C57BL/6J mice.
abstract: To investigate the hypocholesterolemic mechanism of barley in vivo, six-week-old C57BL/6J mice were fed a high-fat diet (HFD) or high-fat diet containing barley (HFD-B) for seven weeks. Total and LDL cholesterol concentrations were significantly reduced in the HFD-B group while fecal cholesterol and bile acid was increased. Real-time PCR and immunoblot analysis revealed the induction of FXR expression, which in turn suppressed the expression of ASBT and NPC1L1 in the HFD-B group compared with the controls. In the liver, the expression of HMG-CoA reductase was significantly reduced while LDL receptor expression was unaltered in the HFD-B group compared with the controls. Our data suggest that the hypocholesterolemic effects of barley are primarily the result of reduced dietary cholesterol uptake and bile acid resorption. Reduced expression of intestinal ASBT and NPC1L1 may play a key role in the regulation of dietary cholesterol and bile acid metabolism in mice consuming a diet containing barley.
authors: Division of Food Bioscience and Technology, College of Life Sciences and Biotechnology, Korea University, Seoul 136-713, South Korea.
authors: Minh-Hien
authors: MH
authors: Hoang
authors: Soung-Jin
authors: SJ
authors: Houng
authors: Hee-Jin
authors: HJ
authors: Jun
authors: Ji-Hae
authors: JH
authors: Lee
authors: Jin-Woong
authors: JW
authors: Choi
authors: So-Hee
authors: SH
authors: Kim
authors: Yong-Ro
authors: YR
authors: Kim
authors: Sung-Joon
authors: SJ
authors: Lee