title: Microsatellite primers in the Peltigera dolichorhiza complex (lichenized ascomycete, Peltigerales).
abstract: OBJECTIVE
  Microsatellite primers were developed for the lichen-forming fungus Peltigera dolichorhiza to investigate partitioning of genetic variation in a widespread, morphologically and chemically variable taxon likely to represent a complex of cryptic lineages, including P. neopolydactyla. •
  
  
  RESULTS
  Using next generation shotgun sequence reads, 331 primer pairs were designed to amplify microsatellite sequences from an African accession of P. dolichorhiza. Eleven primer pairs representing the longest repeat units identified were tested on 15 P. dolichorhiza accessions from Africa (incl. Réunion), South America, Papua New Guinea, and on two accessions of P. neopolydactyla from North America. The primers amplified di-, tri, tetra-, and pentanucelotide repeats with 3-8 alleles per locus. All individuals represent distinct multiloci genotypes. •
  
  
  CONCLUSIONS
  These results indicate the utility of the new microsatellite primers for testing genetic differentiation within the widespread complex of P. dolichorhiza.
authors: Plant Taxonomy & Conservation Biology Unit, University of Liège, Sart Tilman B22, B-4000 Liège, Belgium.
authors: Nicolas
authors: N
authors: Magain
authors: Laura L
authors: LL
authors: Forrest
authors: Emmanuël
authors: E
authors: Sérusiaux
authors: Bernard
authors: B
authors: Goffinet