title: Genotyping of Mycobacterium leprae from Brazilian leprosy patients suggests the occurrence of reinfection or of bacterial population shift during disease relapse.
abstract: We performed genotyping of Mycobacterium leprae present in skin biopsy samples that were collected during the first and the second disease occurrences from eight leprosy patients, seven of whom were diagnosed as suffering from disease relapse. Sequence analysis of part of the M. leprae rpoB, folP1, gyrB and gyrA genes did not show genetic change that supported the presence of drug-resistant bacilli. However, we observed a synonymous nucleotide change at position 297 of gyrA among five of these patients, one presenting C to T (CgyrAT) and four presenting T to C (TgyrAC) at this position. Additional genotyping by analysis of the four short tandem repeats GAA, GTA9, AT17 and TA18 showed that the gyrA single nucleotide polymorphism change was accompanied by a change in short tandem repeat genotype. Our data suggest that leprosy relapse in these patients, living in an area endemic for leprosy, could be caused by M. leprae with a genotype different from the one that caused initial disease.
authors: Laboratory of Molecular Biology Applied to Mycobacteria, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro, RJ, Brazil.
authors: Adalgiza
authors: A
authors: da Silva Rocha
authors: Laboratory of Molecular Biology Applied to Mycobacteria, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro, RJ, Brazil.
authors: Alexandre Araujo
authors: AA
authors: Cunha Dos Santos
authors: Leprosy Laboratory, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro, RJ, Brazil.
authors: Patrícia
authors: P
authors: Pignataro
authors: Leprosy Laboratory, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro, RJ, Brazil.
authors: José Augusto
authors: JA
authors: Nery
authors: Laboratory of Computational and Systems Biology, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro, RJ, Brazil.
authors: Antônio Basílio
authors: AB
authors: de Miranda
authors: Laboratory of Molecular Biology Applied to Mycobacteria, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro, RJ, Brazil.
authors: Diego Fonseca
authors: DF
authors: Soares
authors: Laboratory of Molecular Biology Applied to Mycobacteria, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro, RJ, Brazil.
authors: Amanda Nogueira
authors: AN
authors: Brum Fontes
authors: Leprosy Laboratory, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro, RJ, Brazil.
authors: Alice
authors: A
authors: Miranda
authors: Leprosy Laboratory, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro, RJ, Brazil.
authors: Helen
authors: H
authors: Ferreira
authors: Multidisciplinary Laboratory, University Hospital Clementino Fraga Filho, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors: Neio
authors: N
authors: Boéchat
authors: Leprosy Laboratory, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro, RJ, Brazil.
authors: Maria Eugênia
authors: ME
authors: Novisck Gallo
authors: Leprosy Laboratory, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro, RJ, Brazil.
authors: Euzenir Nunes
authors: EN
authors: Sarno
authors: Training Center on Dermatology, University Hospital Clementino Fraga Filho, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors: Maria Leide W
authors: MLW
authors: De Oliveira
authors: Laboratory of Molecular Biology Applied to Mycobacteria, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro, RJ, Brazil.
authors: Philip Noel
authors: PN
authors: Suffys
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