title: A novel liquid-phase piezoelectric immunosensor for detecting Schistosoma japonicum circulating antigen.
abstract: OBJECTIVE
  A new liquid-phase piezoelectric immunosensor (LP-PEIS), which can detect Schistosoma japonicum (Sj) circulating antigens (SjCAg) quantificationally, was developed.
  
  
  METHODS
  The IgG antibodies were purified from the sera of rabbits which had been infected or immunized by Sj and were immobilized on the surface of piezoelectric quartz crystal in LP-PEIS by staphylococcal protein A (SPA). It was used to detect SjCAg in sera of rabbits which had been infected by Sj in order to acquire some optimum conditions for detecting SjCAg. Finally, the LP-PEIS with optimum conditions was used to detect SjCAg in sera of patients who had been infected by Sj, and was compared with sandwich ELISA.
  
  
  RESULTS
  A lot of optimum conditions of LP-PEIS for detecting SjCAg had been acquired. In the detection of patients' sera with acute Schistosomiasis, LP-PEIS has higher positive rate (100%) and lower false positive rate (3.0%) than sandwich ELISA (92.8%, 6.0%). However, there were no significant difference between LP-PEIS and sandwich ELISA.
  
  
  CONCLUSIONS
  LP-PEIS can quantificationally detect SjCAg in patients' sera as well as sandwich ELISA.
authors: Infectious Disease Department, Nanchang NO.1 Hospital ( NO.3 affiliated hospital of Nanchang University), Nanchang, China. wenzhili@126.com
authors: Zhili
authors: Z
authors: Wen
authors: Shiping
authors: S
authors: Wang
authors: Zhaoyang
authors: Z
authors: Wu
authors: Guoli
authors: G
authors: Shen