title: Thyroid hormone enhances the ability of serum to accept cellular cholesterol via the ABCA1 transporter.
abstract: OBJECTIVE
  The goal of this study was to examine the effects of thyroid hormone status on the ability of serum to accept cellular cholesterol.
  
  
  RESULTS
  Sera from hypophysectomized rats treated ± T(3) was used to evaluate the role of thyroid hormone on serum efflux capacity. 2D-DIGE analysis of serum proteins showed that T(3) treated rats had increased ApoA-I, ApoA-IV and fetuin A levels with decreased Apo E levels. Microarray and real-time RT-PCR analysis of rat liver revealed large increases in ApoA-I, ApoA-IV, ABCG5, and ABCG8 in response to T(3). J774 macrophages, BHK cells, and Fu5AH rat hepatoma cells were used to measure cholesterol efflux mediated by ABCA1, ABCG1 transporters or SR-BI. Sera from T(3)-treated rats stimulated efflux via ABCA1 but not by ABCG1 or SR-BI. Gel filtration chromatography revealed that T(3) treatment caused a decrease in HDL particle size accompanied by higher levels of lipid-poor ApoA-I.
  
  
  CONCLUSIONS
  Thyroid hormone enhances the ability of serum to accept cellular cholesterol via the ABCA1 transporter. This effect is most likely attributable to increases in small HDL and lipid poor ApoA-I in response to T(3).
authors: Department of Molecular Medicine, College of Medicine, University of South Florida, Tampa, FL 33612, USA.
authors: Lindsey R
authors: LR
authors: Boone
authors: William R
authors: WR
authors: Lagor
authors: Margarita de la Llera
authors: Mde L
authors: Moya
authors: Melissa I
authors: MI
authors: Niesen
authors: George H
authors: GH
authors: Rothblat
authors: Gene C
authors: GC
authors: Ness
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