title: Evaluation and decontamination of crude oil-polluted soils using Centrosema pubescen Benth and amendment-support options.
abstract: Growth performance and phytoremediation of soil of the Niger Delta Region of Nigeria artificially-contaminated with crude oil (up to 100 mL/2 kg soil) using centrosema pubescen Benth was investigated for 12 weeks. The soil samples in which the plants were established were either un-amended, or amended with NPK, or UREA or chicken manure. The extents of removal of PAHs and BTEX were measured as well as the rates of growth of the plants. Gas Chromatographic analysis confirmed the degradation of carcinogenic hydrocarbons like BTEXs and PAHs with this technique. At the highest dose of crude, the contaminant concentrations were 43 mg/kg PAHs, 10 mg/kg BTEX, and 5,613 mg/kg O&G. The greatest percent removal of BTEX was observed at the highest contaminant dose, and with the manure amendment. Similar trends were observed with PAHs and although they were less marked, the trends with PAHs may have been more highly statistically significant. There was no measurable plant uptake of contaminants. Inhibition of plant growth (measured as leaf area, shoot length and production of dry weight) was proportional to the dose of crude oil, but the manure amendment was very effective at reducing the growth inhibition. Interestingly, manure amendment reduced the phytotoxicity significantly in this study.
authors: Department of Biochemistry, University of Port Harcourt, Rivers State, Nigeria. nodullm@yahoo.com
authors: Eucharia O
authors: EO
authors: Nwaichi
authors: Leo C
authors: LC
authors: Osuji
authors: Eugene N
authors: EN
authors: Onyeike