title: A new colour assay for [URE3] prion in a genetic background used to score for the [PSI⁺] prion.
abstract: Most scoring assays for yeast prions are dependent on specific genetic markers and constructs that differ for each prion. Here we describe a simple colour assay for the [URE3] prion that works in the 74D-964 strain frequently used to score the [PSI(+)] prion. Although this assay can only be used to score for [URE3] in the [psi(-)] version of the strain, it makes it easier to examine the effects of host mutations or environmental changes on [URE3] or [PSI(+)] using a colour assay in the identical genetic background.
authors: Laboratory for Molecular Biology, Department of Biological Sciences, University of Illinois at Chicago, Chicago, USA.
authors: Joo Y
authors: JY
authors: Hong
authors: Vidhu
authors: V
authors: Mathur
authors: Susan W
authors: SW
authors: Liebman
references: Yeast. 1995 Dec;11(16):1671-85
references: 8720070
references: Microbiol Mol Biol Rev. 1999 Dec;63(4):844-61, table of contents
references: 10585968
references: Curr Genet. 2010 Oct;56(5):467-78
references: 20668856
references: Science. 1994 Apr 22;264(5158):566-9
references: 7909170
references: Mol Cell Biol. 2002 Jun;22(11):3590-8
references: 11997496
references: Genes Dev. 2009 Oct 1;23(19):2320-32
references: 19797769
references: Mol Cell. 2000 Jan;5(1):163-72
references: 10678178
references: Nat Genet. 2008 Apr;40(4):460-5
references: 18362884
references: Science. 1982 Apr 9;216(4542):136-44
references: 6801762
references: Science. 1995 May 12;268(5212):880-4
references: 7754373
references: Proc Natl Acad Sci U S A. 2010 Jun 8;107(23):10573-7
references: 20498075
references: J Mol Biol. 1970 Sep 14;52(2):323-35
references: 5485912
references: Methods. 2006 May;39(1):35-42
references: 16762564
references: Proc Natl Acad Sci U S A. 2000 Jan 4;97(1):240-4
references: 10618402
references: FEMS Microbiol Rev. 2002 Aug;26(3):223-38
references: 12165425
references: FEMS Yeast Res. 2006 Aug;6(5):777-91
references: 16879428
references: Genetics. 1996 Dec;144(4):1375-86
references: 8978027
references: Mol Gen Genet. 1975;136(4):327-35
references: 16095000
references: Proc Natl Acad Sci U S A. 1976 Oct;73(10):3651-5
references: 790391
references: Genetics. 1997 Oct;147(2):507-19
references: 9335589
references: Genetika. 1988 Jul;24(7):1159-65
references: 3053330
references: Nat Cell Biol. 2009 Mar;11(3):344-9
references: 19219034
references: Mol Cell Biol. 2001 Oct;21(20):7035-46
references: 11564886
references: Cell. 2009 Apr 3;137(1):146-58
references: 19345193
references: Nature. 2005 Sep 8;437(7056):262-5
references: 16148935
references: Biochem Soc Trans. 2006 Nov;34(Pt 5):786-90
references: 17052198
references: Mol Cell Biol. 2000 Dec;20(23):8916-22
references: 11073991
references: Proc Natl Acad Sci U S A. 2009 Feb 10;106(6):1892-6
references: 19174511