title: Sphingosine-1-phosphate augments agonist-mediated contraction in the bronchial smooth muscles of mice.
abstract: The effects of sphingosine-1-phosphate (S1P) on bronchial smooth muscle (BSM) contractility were investigated in naive mice. S1P had no effect on the basal tone of the isolated BSM tissues. However, in the presence of S1P (10(-6) M), the BSM contractions induced by acetylcholine (ACh) and endothelin-1 (ET-1) were significantly augmented: both the ACh and ET-1 concentration-response curves were significantly shifted to the left. In contrast, the pretreatment with S1P had no effect on the contractions induced by high K(+) depolarization. It is thus possible that S1P augments BSM contraction induced by the activation of G protein-coupled receptors.
authors: Department of Pharmacology, School of Pharmacy, Hoshi University, 2-4-41 Ebara, Shinagawa-ku, Tokyo 142-8501, Japan. chiba@hoshi.ac.jp
authors: Yoshihiko
authors: Y
authors: Chiba
authors: Hiroki
authors: H
authors: Takeuchi
authors: Hiroyasu
authors: H
authors: Sakai
authors: Miwa
authors: M
authors: Misawa