title: The transcriptome of human epicardial, mediastinal and subcutaneous adipose tissues in men with coronary artery disease.
abstract: BACKGROUND
  The biological functions of epicardial adipose tissue (EAT) remain largely unknown. However, the proximity of EAT to the coronary arteries suggests a role in the pathogenesis of coronary artery disease (CAD). The objectives of this study were to identify genes differentially regulated among three adipose tissues, namely EAT, mediastinal (MAT) and subcutaneous (SAT) and to study their possible relationships with the development of cardiovascular diseases.
  
  
  RESULTS
  Samples were collected from subjects undergoing coronary artery bypass grafting surgeries. Gene expression was evaluated in the three adipose depots of six men using the Illumina® HumanWG-6 v3.0 expression BeadChips. Twenty-three and 73 genes were differentially up-regulated in EAT compared to MAT and SAT, respectively. Ninety-four genes were down-regulated in EAT compared to SAT. However, none were significantly down-regulated in EAT compared to MAT. More specifically, the expression of the adenosine A1 receptor (ADORA1), involved in myocardial ischemia, was significantly up-regulated in EAT. Levels of the prostaglandin D2 synthase (PTGDS) gene, recently associated with the progression of atherosclerosis, were significantly different in the three pairwise comparisons (EAT>MAT>SAT). The results of ADORA1 and PTGDS were confirmed by quantitative real-time PCR in 25 independent subjects.
  
  
  CONCLUSIONS
  Overall, the transcriptional profiles of EAT and MAT were similar compared to the SAT. Despite this similarity, two genes involved in cardiovascular diseases, ADORA1 and PTGDS, were differentially up-regulated in EAT. These results provide insights about the biology of EAT and its potential implication in CAD.
authors: Centre de Recherche Institut Universitaire de Cardiologie et de Pneumologie de Québec, Laval University, Quebec, Canada.
authors: Sandra
authors: S
authors: Guauque-Olarte
authors: Nathalie
authors: N
authors: Gaudreault
authors: Marie-Ève
authors: MÈ
authors: Piché
authors: Dominique
authors: D
authors: Fournier
authors: Pascale
authors: P
authors: Mauriège
authors: Patrick
authors: P
authors: Mathieu
authors: Yohan
authors: Y
authors: Bossé
references: Am J Physiol Heart Circ Physiol. 2003 Mar;284(3):H859-66
references: 12578815
references: Clin Pharmacol Ther. 2010 Apr;87(4):407-16
references: 20200516
references: Arterioscler Thromb Vasc Biol. 2008 Jul;28(7):1304-10
references: 18420999
references: J Nutr. 2004 Apr;134(4):762-70
references: 15051823
references: Hypertens Res. 2008 Oct;31(10):1931-9
references: 19015601
references: Am J Cardiol. 2008 Dec 15;102(12):1602-7
references: 19064012
references: Diabetes. 1998 Sep;47(9):1384-91
references: 9726225
references: Proc Natl Acad Sci U S A. 1997 Jun 10;94(12):6541-6
references: 9177254
references: Am Heart J. 2007 Jun;153(6):907-17
references: 17540190
references: Metabolism. 2010 Sep;59(9):1379-86
references: 20116810
references: J Clin Endocrinol Metab. 2010 Feb;95(2):963-7
references: 20008021
references: Hypertension. 2006 Jun;47(6):1140-6
references: 16636200
references: Nucleic Acids Res. 2002 Jan 1;30(1):207-10
references: 11752295
references: Horm Metab Res. 2001 Oct;33(10):625-7
references: 11607884
references: Int J Cardiol. 2007 Sep 14;121(1):132-4
references: 17107724
references: Obes Rev. 2007 May;8(3):253-61
references: 17444966
references: J Biol Chem. 2003 Jun 13;278(24):22175-81
references: 12684506
references: Metabolism. 1991 Sep;40(9):990-6
references: 1895966
references: J Lipid Res. 1999 Mar;40(3):397-404
references: 10064727
references: Diabetes. 1998 Jan;47(1):98-103
references: 9421381
references: Metab Syndr Relat Disord. 2009 Apr;7(2):125-31
references: 19422139
references: Nucleic Acids Res. 2006 Jul 1;34(Web Server issue):W645-50
references: 16912992
references: Nature. 2006 Dec 14;444(7121):881-7
references: 17167477
references: J Clin Endocrinol Metab. 2006 May;91(5):1882-6
references: 16507638
references: Metabolism. 1993 Jun;42(6):739-42
references: 8510519
references: Am J Physiol Heart Circ Physiol. 2010 Mar;298(3):H746-53
references: 20061546
references: Semin Immunopathol. 2009 Jun;31(1):23-33
references: 19340429
references: Eur J Echocardiogr. 2009 Jun;10(4):549-55
references: 19211568
references: Cytokine. 2010 Aug;51(2):207-12
references: 20610178
references: Circ J. 2007 Apr;71(4):536-9
references: 17384455
references: J Clin Endocrinol Metab. 2009 Jan;94(1):261-7
references: 18984670
references: J Huazhong Univ Sci Technolog Med Sci. 2010 Oct;30(5):589-93
references: 21063839
references: Nat Clin Pract Cardiovasc Med. 2005 Oct;2(10):536-43
references: 16186852
references: Science. 2010 Jan 8;327(5962):217-20
references: 19965390
references: J Clin Endocrinol Metab. 2003 Nov;88(11):5163-8
references: 14602744
references: Atherosclerosis. 2008 Dec;201(2):385-91
references: 18436228
references: Obes Res. 2004 Aug;12(8):1217-22
references: 15340102
references: Obesity (Silver Spring). 2008 Apr;16(4):887-92
references: 18379565
references: J Lipid Res. 2004 Jan;45(1):148-54
references: 14563828
references: Int J Obes Relat Metab Disord. 1996 Feb;20(2):185-9
references: 8646256
references: Rev Esp Cardiol. 2006 Dec;59(12):1252-60
references: 17194420
references: J Cardiovasc Pharmacol. 2009 May;53(5):424-33
references: 19333129
references: J Lipid Res. 1995 Apr;36(4):672-84
references: 7616115
references: J Biol Chem. 2005 Aug 19;280(33):29946-55
references: 15970590
references: Proc Natl Acad Sci U S A. 2001 Apr 24;98(9):5116-21
references: 11309499
references: Circulation. 2006 Nov 21;114(21):2240-50
references: 17088462
references: Curr Atheroscler Rep. 2010 Mar;12(2):125-33
references: 20425248
references: J Pathol. 1998 May;185(1):4-9
references: 9713353
references: Biol Cell. 2004 Jun;96(5):343-8
references: 15207902
references: Clin Pharmacol Ther. 2006 Mar;79(3):173-85
references: 16513442
references: J Clin Endocrinol Metab. 1997 Dec;82(12):4162-6
references: 9398732
references: Acta Physiol Scand. 1991 Jul;142(3):405-10
references: 1927553
references: Br J Pharmacol. 2004 Mar;141(5):851-9
references: 14769782
references: Hypertension. 2002 Feb;39(2 Pt 2):449-54
references: 11882588
references: Circulation. 2003 Nov 18;108(20):2460-6
references: 14581396
references: Bioinformatics. 2003 Jan 22;19(2):185-93
references: 12538238
references: Bioinformatics. 2008 Jul 1;24(13):1547-8
references: 18467348
references: Expert Opin Investig Drugs. 2008 Dec;17(12):1901-10
references: 19012505
references: Genome Res. 2003 Sep;13(9):2129-41
references: 12952881
references: J Am Soc Echocardiogr. 2009 Dec;22(12):1311-9; quiz 1417-8
references: 19944955
references: Eur J Clin Invest. 1987 Apr;17(2):156-65
references: 3034620
references: Nutr Metab Cardiovasc Dis. 2009 Dec;19(11):760-6
references: 19362807
references: Int J Biochem Cell Biol. 2008;40(5):821-36
references: 18201922
references: Nucleic Acids Res. 2009 Jul;37(Web Server issue):W305-11
references: 19465376
references: Arterioscler Thromb Vasc Biol. 2004 Jul;24(7):1259-65
references: 15155382
references: Eur Heart J. 2009 Apr;30(7):850-6
references: 19136488