title: The rice mitochondrial iron transporter is essential for plant growth.
abstract: In plants, iron (Fe) is essential for mitochondrial electron transport, heme, and Fe-Sulphur (Fe-S) cluster synthesis; however, plant mitochondrial Fe transporters have not been identified. Here we show, identify and characterize the rice mitochondrial Fe transporter (MIT). Based on a transfer DNA library screen, we identified a rice line showing symptoms of Fe deficiency while accumulating high shoot levels of Fe. Homozygous knockout of MIT in this line resulted in a lethal phenotype. MIT localized to the mitochondria and complemented the growth of Δmrs3Δmrs4 yeast defective in mitochondrial Fe transport. The growth of MIT-knockdown (mit-2) plants was also significantly impaired despite abundant Fe accumulation. Further, the decrease in the activity of the mitochondrial and cytosolic Fe-S enzyme, aconitase, indicated that Fe-S cluster synthesis is affected in mit-2 plants. These results indicate that MIT is a mitochondrial Fe transporter essential for rice growth and development.
authors: Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Tokyo 113-8657, Japan.
authors: Khurram
authors: K
authors: Bashir
authors: Yasuhiro
authors: Y
authors: Ishimaru
authors: Hugo
authors: H
authors: Shimo
authors: Seiji
authors: S
authors: Nagasaka
authors: Masaru
authors: M
authors: Fujimoto
authors: Hideki
authors: H
authors: Takanashi
authors: Nobuhiro
authors: N
authors: Tsutsumi
authors: Gynheung
authors: G
authors: An
authors: Hiromi
authors: H
authors: Nakanishi
authors: Naoko K
authors: NK
authors: Nishizawa
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