title: Growth phase-dependent gene regulation in vivo in Sulfolobus solfataricus.
abstract: Ribosomal genes are strongly regulated dependent on growth phase in all organisms, but this regulation is poorly understood in Archaea. Moreover, very little is known about growth phase-dependent gene regulation in Archaea. SSV1-based lacS reporter gene constructs containing the Sulfolobus 16S/23S rRNA gene core promoter, the TF55α core promoter, or the native lacS promoter were tested in Sulfolobus solfataricus cells lacking the lacS gene. The 42-bp 16S/23S rRNA gene and 39-bp TF55α core promoters are sufficient for gene expression in S. solfataricus. However, only gene expression driven by the 16S/23S rRNA gene core promoter is dependent on the culture growth phase. This is the smallest known regulated promoter in Sulfolobus. To our knowledge, this is the first study to show growth phase-dependent rRNA gene regulation in Archaea.
authors: Biology Department and Center for Life in Extreme Environments, Portland State University, Portland, OR 97207, USA.
authors: Melissa
authors: M
authors: DeYoung
authors: Michelle
authors: M
authors: Thayer
authors: John
authors: J
authors: van der Oost
authors: Kenneth M
authors: KM
authors: Stedman