title: Mitochondrial RNA processing in trypanosomes.
abstract: The mitochondrial genome of trypanosomes is composed of ∼50 maxicircles and thousands of minicircles. Maxi-(∼25 kb) and mini-(∼1 kb)circles are catenated and packed into a dense structure called a kinetoplast. Both types of circular DNA are transcribed by a phage-like RNA polymerase: maxicircles yield multicistronic rRNA and mRNA precursors, while guide RNA (gRNA) precursors are produced from minicircles. To function in mitochondrial translation, pre-mRNAs must undergo a nucleolytic processing and 3' modifications, and often uridine insertion/deletion editing. gRNAs, which represent short (50-60 nt) RNAs directing editing reactions, are produced by 3' nucleolytic processing of a much longer precursor followed by 3' uridylation. Ribosomal RNAs are excised from precursors and their 3' ends are also trimmed and uridylated. All tRNAs are imported from the cytoplasm and some are further modified and edited in the mitochondrial matrix. Historically, the fascinating phenomenon of RNA editing has been extensively studied as an isolated pathway in which nuclear-encoded proteins mediate interactions of maxi- and minicircle transcripts to create open reading frames. However, recent studies unraveled a highly integrated network of mitochondrial genome expression including critical pre- and post-editing 3' mRNA processing, and gRNA and rRNA maturation steps. Here we focus on RNA 3' adenylation and uridylation as processes essential for biogenesis, stability and functioning of mitochondrial RNAs.
authors: Department of Microbiology and Molecular Genetics, School of Medicine, University of California, B240 Medical Sciences I, Irvine, CA 92697, USA. ruslan@uci.edu
authors: Ruslan
authors: R
authors: Aphasizhev
authors: Inna
authors: I
authors: Aphasizheva
references: RNA. 2003 Apr;9(4):484-92
references: 12649499
references: Mol Cell Biol. 1991 Dec;11(12):5878-84
references: 1719373
references: Mol Biochem Parasitol. 1992 Dec;56(2):289-99
references: 1336570
references: Eukaryot Cell. 2006 Jan;5(1):45-53
references: 16400167
references: Science. 2010 Nov 12;330(6006):920-1
references: 21071654
references: EMBO J. 2005 Dec 7;24(23):4029-40
references: 16270030
references: Nucleic Acids Res. 1999 Mar 1;27(5):1377-85
references: 9973629
references: Nucleic Acids Res. 2001 Jul 15;29(14):2950-62
references: 11452020
references: J Biol Chem. 2003 Aug 29;278(35):32753-62
references: 12801929
references: Mol Cell. 2011 Apr 8;42(1):106-17
references: 21474072
references: J Clin Invest. 2008 Apr;118(4):1301-10
references: 18382742
references: Mol Biochem Parasitol. 1992 Jun;52(2):231-40
references: 1352374
references: Mol Cell Biol. 2000 Apr;20(7):2308-16
references: 10713154
references: Cell. 1992 Aug 7;70(3):459-67
references: 1379519
references: RNA. 2009 Jul;15(7):1322-37
references: 19465686
references: Trends Biochem Sci. 2000 Feb;25(2):46-7
references: 10664580
references: J Mol Biol. 2010 Jun 11;399(3):464-75
references: 20403364
references: J Biol Chem. 2000 Dec 22;275(51):40174-9
references: 11013266
references: RNA. 2009 Feb;15(2):277-86
references: 19096045
references: Nucleic Acids Res. 2007;35(5):1544-54
references: 17287292
references: Mol Cell Biol. 2005 Mar;25(5):1634-44
references: 15713623
references: RNA. 2005 May;11(5):763-73
references: 15811918
references: J Biol Chem. 1998 Aug 21;273(34):21825-33
references: 9705321
references: Cell Mol Life Sci. 2005 Oct;62(19-20):2194-203
references: 16158189
references: Cell. 2002 Mar 8;108(5):637-48
references: 11893335
references: Gene. 2001 Jul 11;272(1-2):209-18
references: 11470527
references: Wiley Interdiscip Rev RNA. 2011 Sep-Oct;2(5):669-85
references: 21823228
references: RNA Biol. 2008 Apr-Jun;5(2):84-91
references: 18418086
references: Cell. 1990 Jul 27;62(2):391-7
references: 1695552
references: EMBO J. 2009 Jul 22;28(14):2042-52
references: 19424177
references: Trends Plant Sci. 2009 Sep;14(9):497-504
references: 19716749
references: Proc Natl Acad Sci U S A. 2011 Jan 4;108(1):415-20
references: 21173259
references: Eukaryot Cell. 2004 Aug;3(4):862-9
references: 15302819
references: Annu Rev Plant Biol. 2010;61:125-55
references: 20192740
references: Trends Plant Sci. 2008 Dec;13(12):663-70
references: 19004664
references: Mol Biochem Parasitol. 2006 Mar;146(1):68-77
references: 16310873
references: Mol Biochem Parasitol. 1986 Mar;18(3):321-31
references: 3960057
references: J Biol Chem. 1998 Nov 6;273(45):30003-11
references: 9792721
references: Mol Cell. 2008 Oct 24;32(2):198-209
references: 18951088
references: Mol Biochem Parasitol. 1992 Aug;54(1):31-41
references: 1381496
references: Mol Cell Biol. 2010 Mar;30(6):1555-67
references: 20086102
references: Biochem Soc Trans. 2007 Dec;35(Pt 6):1643-7
references: 18031283
references: Biochem Soc Trans. 2005 Nov;33(Pt 5):967-71
references: 16246022
references: Proc Natl Acad Sci U S A. 2003 Sep 16;100(19):10617-22
references: 12954983
references: RNA Biol. 2010 Mar-Apr;7(2):229-36
references: 20220308
references: Nucleic Acids Res. 2000 Mar 1;28(5):1266-75
references: 10666472
references: Protist. 2003 Jul;154(2):265-79
references: 13677453
references: Mol Biochem Parasitol. 2001 Jun;115(1):109-17
references: 11377745
references: Cell. 1990 Jan 26;60(2):189-98
references: 1688737
references: Mol Biochem Parasitol. 1994 Dec;68(2):297-306
references: 7739675
references: EMBO J. 2000 Oct 16;19(20):5525-32
references: 11032819
references: RNA. 2007 Jan;13(1):1-4
references: 17123956
references: J Biol Chem. 2002 May 10;277(19):16952-9
references: 11859084
references: EMBO J. 2008 Jun 4;27(11):1596-608
references: 18464794
references: J Mol Biol. 2007 Feb 23;366(3):882-99
references: 17189640
references: Mol Biochem Parasitol. 2009 Mar;164(1):66-73
references: 19070634
references: Science. 2001 Mar 16;291(5511):2159-62
references: 11251122
references: J Biol Chem. 2001 Jun 15;276(24):21280-4
references: 11279235
references: J Biol Chem. 1989 Nov 5;264(31):18679-86
references: 2478548
references: J Biol Chem. 1999 Apr 23;274(17):12067-74
references: 10207031
references: Mol Biochem Parasitol. 2007 Aug;154(2):158-69
references: 17543398
references: Cell. 1987 May 8;49(3):337-45
references: 3568129
references: Mol Cell Proteomics. 2008 Mar;7(3):534-45
references: 18073385
references: Trends Parasitol. 2005 Aug;21(8):363-9
references: 15967722
references: Biochim Biophys Acta. 2008 Apr;1779(4):270-80
references: 18191648
references: Nucleic Acids Res. 2008 Jan;36(1):319-29
references: 18032430
references: RNA. 2009 Apr;15(4):588-99
references: 19228586
references: Mol Biochem Parasitol. 1991 Oct;48(2):139-49
references: 1837067
references: Proc Natl Acad Sci U S A. 2007 Sep 11;104(37):14634-9
references: 17785418
references: FEBS Lett. 2004 Aug 13;572(1-3):15-8
references: 15304317
references: Biol Chem. 2009 Aug;390(8):717-22
references: 19558325
references: Science. 2005 Jul 15;309(5733):404-9
references: 16020724
references: Mol Cell Proteomics. 2008 Jul;7(7):1286-96
references: 18364347
references: Mol Cell Biol. 2007 Oct;27(19):6876-88
references: 17646387
references: Mol Biochem Parasitol. 1997 Dec 1;90(1):81-94
references: 9497034
references: Cell. 1990 Nov 16;63(4):783-90
references: 2171782
references: EMBO J. 2003 Feb 17;22(4):913-24
references: 12574127
references: J Biol Chem. 2010 Jan 8;285(2):1220-8
references: 19850921