title: (31)P NMR correlation maps of (18)O/ (16)O chemical shift isotopic effects for phosphometabolite labeling studies.
abstract: Intramolecular correlations among the (18)O-labels of metabolic oligophosphates, mapped by J-decoupled (31)P NMR 2D chemical shift correlation spectroscopy, impart stringent constraints to the (18)O-isotope distributions over the whole oligophosphate moiety. The multiple deduced correlations of isotopic labels enable determination of site-specific fractional isotope enrichments and unravel the isotopologue statistics. This approach ensures accurate determination of (18)O-labeling rates of phosphometabolites, critical in biochemical energy conversion and metabolic flux transmission. The biological usefulness of the J-decoupled (31)P NMR 2D chemical shift correlation maps was validated on adenosine tri-phosphate fractionally (18)O labeled in perfused mammalian hearts.
authors: Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN, 55905, USA. juranic.nenad@mayo.edu
authors: Nenad
authors: N
authors: Juranić
authors: Emirhan
authors: E
authors: Nemutlu
authors: Song
authors: S
authors: Zhang
authors: Petras
authors: P
authors: Dzeja
authors: Andre
authors: A
authors: Terzic
authors: Slobodan
authors: S
authors: Macura
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