title: A highly selective, cell-permeable fluorescent nanoprobe for ratiometric detection and imaging of peroxynitrite in living cells.
abstract: Peroxynitrite (ONOO(-)) is a highly reactive species implicated in the pathology of numerous diseases and there is currently great interest in developing fluorescent probes that can selectively detect ONOO(-) in living cells. Herein, a polymeric micelle-based and cell-penetrating peptide-coated fluorescent nanoprobe that incorporates ONOO(-) indicator dye and reference dye for the ratiometric detection and imaging of ONOO(-) has been developed. The nanoprobe effectively avoids the influences from enzymatic reaction and high-concentration ·OH and ClO(-). The improved ONOO(-) selectivity of the nanoprobe is achieved by a delicate complementarity of properties between the nanomatrix and the embedded molecular probe (BzSe-Cy). This nanoprobe also has other attractive properties, such as good water solubility, photostability, biocompatibility, and near-infrared excitation and emission. Fluorescence imaging experiments by confocal microscopy show that this nanoprobe is capable of visualizing ONOO(-) produced in living cells and it exhibits very low toxicity and good membrane permeability. We anticipate that this technique will be a potential tool for the precise pathological understanding and diagnosis of ONOO(-)-related human diseases.
authors: College of Chemistry, Chemical Engineering and Materials Science, Key Laboratory of Molecular and Nano Probes, Clean Production, Ministry of Education, Shandong Normal University, Jinan, P R China.
authors: Jiangwei
authors: J
authors: Tian
authors: Huachao
authors: H
authors: Chen
authors: Linhai
authors: L
authors: Zhuo
authors: Yanxia
authors: Y
authors: Xie
authors: Na
authors: N
authors: Li
authors: Bo
authors: B
authors: Tang