title: Optimization of electrical stimulation parameters for cardiac tissue engineering.
abstract: In vitro application of pulsatile electrical stimulation to neonatal rat cardiomyocytes cultured on polymer scaffolds has been shown to improve the functional assembly of cells into contractile engineered cardiac tissues. However, to date, the conditions of electrical stimulation have not been optimized. We have systematically varied the electrode material, amplitude and frequency of stimulation to determine the conditions that are optimal for cardiac tissue engineering. Carbon electrodes, exhibiting the highest charge-injection capacity and producing cardiac tissues with the best structural and contractile properties, were thus used in tissue engineering studies. Engineered cardiac tissues stimulated at 3 V/cm amplitude and 3 Hz frequency had the highest tissue density, the highest concentrations of cardiac troponin-I and connexin-43 and the best-developed contractile behaviour. These findings contribute to defining bioreactor design specifications and electrical stimulation regime for cardiac tissue engineering.
authors: Department of Biomedical Engineering, Columbia University, New York, NY 10027, USA.
authors: Nina
authors: N
authors: Tandon
authors: Anna
authors: A
authors: Marsano
authors: Robert
authors: R
authors: Maidhof
authors: Leo
authors: L
authors: Wan
authors: Hyoungshin
authors: H
authors: Park
authors: Gordana
authors: G
authors: Vunjak-Novakovic
references: J Neurosci Methods. 2005 Feb 15;141(2):171-98
references: 15661300
references: J Mol Cell Cardiol. 1991 Feb;23(2):161-73
references: 2067025
references: J Tissue Eng Regen Med. 2008 Jul;2(5):279-87
references: 18512267
references: Science. 1912 Jul 19;36(916):90-2
references: 17736724
references: Biotechnol Prog. 2008 Jul-Aug;24(4):907-20
references: 19194900
references: Cardiovasc Res. 2003 Mar;57(3):793-803
references: 12618241
references: IEEE Eng Med Biol Mag. 2005 Jul-Aug;24(4):73-9
references: 16119216
references: J Biomed Mater Res A. 2008 Sep;86(3):713-24
references: 18041719
references: Circulation. 2009 Jan 27;119(3):e21-181
references: 19075105
references: Nat Protoc. 2009;4(2):155-73
references: 19180087
references: Biomaterials. 2007 Oct;28(29):4277-93
references: 17604100
references: Proc Natl Acad Sci U S A. 2004 Dec 28;101(52):18129-34
references: 15604141
references: Tissue Eng Part A. 2008 Sep;14(9):1461-8
references: 18601591
references: Annu Int Conf IEEE Eng Med Biol Soc. 2008;2008:3594-7
references: 19163486
references: Annu Int Conf IEEE Eng Med Biol Soc. 2009;2009:2756-9
references: 19964592
references: Tissue Eng. 2002 Apr;8(2):175-88
references: 12031108
references: J Orthop Res. 1995 Mar;13(2):258-67
references: 7722763
references: J Membr Biol. 1979 Dec 12;51(1):75-96
references: 522130
references: Am J Physiol. 1993 Aug;265(2 Pt 2):H747-54
references: 8368376
references: Bioessays. 2000 Feb;22(2):188-99
references: 10655038
references: Bioelectromagnetics. 1992;Suppl 1:147-57
references: 1285710
references: Pflugers Arch. 1997 Aug;434(4):438-44
references: 9211810
references: Basic Res Cardiol. 2005 Jul;100(4):337-45
references: 15822004
references: Am J Physiol. 1994 Jan;266(1 Pt 2):H341-9
references: 8304516
references: Biotechniques. 2010 Jan;48(1):65-7
references: 20078430
references: Tissue Eng. 2006 Aug;12(8):2077-91
references: 16968150
references: Circ Res. 2002 Feb 8;90(2):223-30
references: 11834716
references: Am J Physiol Heart Circ Physiol. 2004 Feb;286(2):H507-16
references: 14551059