title: Harnessing yeast subcellular compartments for the production of plant terpenoids.
abstract: The biologically and commercially important terpenoids are a large and diverse class of natural products that are targets of metabolic engineering. However, in the context of metabolic engineering, the otherwise well-documented spatial subcellular arrangement of metabolic enzyme complexes has been largely overlooked. To boost production of plant sesquiterpenes in yeast, we enhanced flux in the mevalonic acid pathway toward farnesyl diphosphate (FDP) accumulation, and evaluated the possibility of harnessing the mitochondria as an alternative to the cytosol for metabolic engineering. Overall, we achieved 8- and 20-fold improvement in the production of valencene and amorphadiene, respectively, in yeast co-engineered with a truncated and deregulated HMG1, mitochondrion-targeted heterologous FDP synthase and a mitochondrion-targeted sesquiterpene synthase, i.e. valencene or amorphadiene synthase. The prospect of harnessing different subcellular compartments opens new and intriguing possibilities for the metabolic engineering of pathways leading to valuable natural compounds.
authors: Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel.
authors: Moran
authors: M
authors: Farhi
authors: Elena
authors: E
authors: Marhevka
authors: Tania
authors: T
authors: Masci
authors: Evgeniya
authors: E
authors: Marcos
authors: Yoram
authors: Y
authors: Eyal
authors: Mariana
authors: M
authors: Ovadis
authors: Hagai
authors: H
authors: Abeliovich
authors: Alexander
authors: A
authors: Vainstein