title: Identification and characterization of 12 microsatellite loci in Cnidium dubium (Apiaceae) using next-generation sequencing.
abstract: OBJECTIVE
  We present a set of 12 polymorphic nuclear microsatellite loci, identified for the first time within the herbaceous plant species Cnidium dubium (Schkuhr) Thell. using next-generation sequencing.
  
  
  RESULTS
  To characterize these loci, 40 plants were analyzed genetically. The number of alleles ranged from 2 to 23, with an average of 8.3 alleles per locus. Mean observed and expected heterozygosities ranged from 0.25 to 0.95 and 0.30 to 0.94, respectively.
  
  
  CONCLUSIONS
  All 12 nuclear microsatellite loci showed scorable and polymorphic fragments after polymerase chain reaction. The new marker set can be used for future studies of genetic diversity and differentiation as well as estimation of gene flow and spatial genetic structures.
authors: University of Marburg, Faculty of Biology, Conservation Biology, Karl-von-Frisch-Strasse 8, 35043 Marburg, Germany. inga.michalczyk@web.de
authors: Inga M
authors: IM
authors: Michalczyk
authors: Claudia
authors: C
authors: Schumacher
authors: Christina
authors: C
authors: Mengel
authors: Ilona
authors: I
authors: Leyer
authors: Sascha
authors: S
authors: Liepelt