title: Identification of a LNCaP-specific binding peptide using phage display.
abstract: OBJECTIVE
  To identify a LNCaP-specific peptide using a phage display library and evaluate its potential applications in targeted drug delivery.
  
  
  METHODS
  Binding abilities of selected phages were evaluated by cell phage ELISA. The KYL peptide encoded by the most specific phage clone was synthesized, labeled with fluorescein, and assayed in various cell lines. A fusion peptide composed of the KYL peptide and a proapoptotic peptide ( D )(KLAKLAK)(2) was synthesized, and the cell death effect was evaluated on different cells. Moreover, the KYL peptide was conjugated to a cationic protein, protamine, to explore its potential application in siRNA delivery.
  
  
  RESULTS
  One phage clone with a high binding affinity to LNCaP cells was identified. Cell phage ELISA and immunostaining demonstrated high specificity of this phage to LNCaP cells. The fluorescein-labeled KYL peptide exhibited higher binding to LNCaP cells in comparison to other cells. The fusion peptide composed of the KYL peptide and the proapoptotic peptide induced cell death in LNCaP cells, but not in PC-3 cells. The KYL peptide-protamine conjugate also efficiently delivered a fluorescein-labeled siRNA into LNCaP cells.
  
  
  CONCLUSIONS
  We identified a LNCaP-specific peptide and demonstrated its potential applications in targeted drug delivery to LNCaP cells.
authors: Division of Pharmaceutical Sciences, School of Pharmacy, University of Missouri-Kansas City, 2464 Charlotte Street, Kansas City, Missouri, 64108, USA.
authors: Bin
authors: B
authors: Qin
authors: Wanyi
authors: W
authors: Tai
authors: Ravi S
authors: RS
authors: Shukla
authors: Kun
authors: K
authors: Cheng
references: Biochemistry. 2006 Aug 8;45(31):9434-44
references: 16878978
references: PLoS One. 2009;4(1):e4171
references: 19137069
references: Endocr Relat Cancer. 2002 Jun;9(2):115-39
references: 12121835
references: Methods Enzymol. 2003;373:332-42
references: 14714413
references: Cancer Res. 2001 Nov 1;61(21):7709-12
references: 11691780
references: Bioconjug Chem. 2007 Nov-Dec;18(6):1831-40
references: 17922544
references: Cancer Res. 2006 Sep 15;66(18):9171-7
references: 16982760
references: J Protein Chem. 2002 May;21(4):287-96
references: 12168699
references: Clin Cancer Res. 1997 Jan;3(1):81-5
references: 9815541
references: Biochemistry. 2010 Feb 23;49(7):1364-76
references: 20102226
references: Prostate. 2009 Jul 1;69(10):1077-90
references: 19343732
references: CA Cancer J Clin. 2007 Jan-Feb;57(1):43-66
references: 17237035
references: Clin Cancer Res. 2008 Sep 1;14(17):5494-502
references: 18765541
references: Neoplasia. 2006 Sep;8(9):772-80
references: 16984734
references: Cancer Cell. 2003 Nov;4(5):393-403
references: 14667506
references: Nature. 1988 Oct 6;335(6190):564-8
references: 3419534
references: Oncogene. 2005 Mar 10;24(11):1847-59
references: 15688028
references: J Control Release. 2004 May 18;96(3):497-507
references: 15120905
references: Leukemia. 2007 Mar;21(3):411-20
references: 17252013
references: Biochem J. 1983 Aug 1;213(2):519-23
references: 6351844
references: J Control Release. 2008 Sep 10;130(2):140-5
references: 18632177
references: Chem Rev. 1997 Apr 1;97(2):391-410
references: 11848876
references: Int J Cancer. 2004 Oct 10;111(6):951-60
references: 15300809
references: Nat Med. 2001 Nov;7(11):1249-53
references: 11689892
references: Cancer Res. 1994 Apr 1;54(7):1807-11
references: 7511053
references: J Urol. 2005 Feb;173(2):342-59
references: 15643172
references: Nat Med. 1999 Sep;5(9):1032-8
references: 10470080
references: Cancer Gene Ther. 2004 Nov;11(11):748-56
references: 15375377
references: Cancer Cell. 2004 Sep;6(3):275-84
references: 15380518
references: J Drug Target. 2005 Jan;13(1):7-18
references: 15848950
references: Bioorg Med Chem Lett. 2008 Mar 1;18(5):1632-6
references: 18243696
references: Nat Biotechnol. 2005 Jun;23(6):709-17
references: 15908939
references: Cancer Gene Ther. 2004 Feb;11(2):128-34
references: 14671672
references: J Mol Med (Berl). 2006 Sep;84(9):764-73
references: 16763842
references: J Control Release. 2010 Sep 15;146(3):264-75
references: 20385184
references: J Labelled Comp Radiopharm. 2009 Dec 1;52(14):583-590
references: 20976123
references: Cancer Res. 1999 Jun 15;59(12):2869-74
references: 10383148
references: J Biol Chem. 2008 Apr 25;283(17):11752-62
references: 18292083
references: J Urol. 2005 Feb;173(2):360-72
references: 15643173
references: Nat Biotechnol. 2006 Aug;24(8):1005-15
references: 16823371