title: Tendon-derived stem cells (TDSCs): from basic science to potential roles in tendon pathology and tissue engineering applications.
abstract: Traditionally, tendons are considered to only contain tenocytes that are responsible for the maintenance, repair and remodeling of tendons. Stem cells, which are termed tendon-derived stem cells (TDSCs), have recently been identified in tendons. This review aims to summarize the current information about the in vitro characteristics of TDSCs, including issues related to TDSC isolation and culture, their cell morphology, immunophenotypes, proliferation and differentiation characteristics and senescence during in vitro passaging. The challenges in studying the functions of these cells are also discussed. The niche where TDSCs resided essentially provides signals that are conducive to the maintenance of definitive stem cell properties of TDSCs. Yet the niche may also induce pathologies by imposing an aberrant function on TDSCs or other targets. The possible niche factors of TDSCs are herein discussed. We presented current evidences supporting the potential pathogenic role of TDSCs in the development of tendinopathy with reference to the recent findings on the altered biological responses of these cells in response to their potential niche factors. The use of resident stem cells may promote engraftment and differentiation of transplanted cells in tendon and tendon-bone junction repair because the tendon milieu is an ideal and familiar environment to the transplanted cells. Evidences are presented to show the potential advantages and results of using TDSCs as a new cell source for tendon and tendon-bone junction repair. Issues pertaining to the use of TDSCs for tissue repair are also discussed.
authors: Department of Orthopaedics and Traumatology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, SAR, China. pauline@ort.cuhk.edu.hk
authors: Pauline Po Yee
authors: PP
authors: Lui
authors: Kai Ming
authors: KM
authors: Chan
references: Proc Natl Acad Sci U S A. 1998 Mar 31;95(7):3908-13
references: 9520466
references: J Bone Joint Surg Am. 2009 Mar 1;91(3):652-63
references: 19255227
references: Arthritis Rheum. 2005 Aug;52(8):2521-9
references: 16052568
references: Biomaterials. 2010 Mar;31(8):2163-75
references: 19995669
references: Clin J Sport Med. 2007 Mar;17(2):129-34
references: 17414481
references: Cancer Res. 2008 Jun 1;68(11):4229-38
references: 18519682
references: J Thorac Cardiovasc Surg. 2000 Nov;120(5):999-1005
references: 11044327
references: Stem Cells. 2006 May;24(5):1294-301
references: 16410387
references: Am J Sports Med. 2010 Apr;38(4):757-64
references: 20139325
references: Clin J Sport Med. 2006 Jul;16(4):329-34
references: 16858217
references: Tissue Eng Part A. 2010 May;16(5):1549-58
references: 20001227
references: Biochem Biophys Res Commun. 2004 Jan 16;313(3):503-8
references: 14697217
references: Cell Biol Int. 2008 Sep;32(9):1150-7
references: 18638562
references: Leukemia. 2010 Dec;24(12):1979-92
references: 20861913
references: J Cutan Pathol. 2008 Sep;35(9):826-31
references: 18422975
references: Annu Rev Biomed Eng. 2004;6:303-29
references: 15255772
references: Clin Orthop Relat Res. 2008 Jul;466(7):1577-82
references: 18425559
references: J Bone Joint Surg Am. 2007 Jan;89(1):74-81
references: 17200313
references: Stem Cells Dev. 2011 Jan;20(1):53-66
references: 20528146
references: J Neurosurg. 2001 Aug;95(2):298-307
references: 11780901
references: J Physiol. 1999 Mar 15;515 ( Pt 3):919-27
references: 10066916
references: Arch Orthop Trauma Surg. 2007 Nov;127(9):815-21
references: 17569067
references: Circulation. 2005 May 10;111(18):2364-72
references: 15851600
references: Nature. 2002 Jul 4;418(6893):41-9
references: 12077603
references: Tissue Eng Part A. 2008 Oct;14(10):1615-27
references: 18759661
references: Bone. 2003 Oct;33(4):475-84
references: 14555250
references: BMC Musculoskelet Disord. 2007 Feb 23;8:16
references: 17319938
references: Tissue Eng. 1999 Jun;5(3):267-77
references: 10434073
references: J Mol Histol. 2007 Oct;38(5):405-13
references: 17668282
references: Congenit Anom (Kyoto). 2002 Mar;42(1):1-9
references: 12094073
references: Proc Natl Acad Sci U S A. 2000 Mar 28;97(7):3213-8
references: 10725391
references: Science. 2005 Jul 8;309(5732):314-7
references: 16002622
references: Carcinogenesis. 2009 Jan;30(1):150-7
references: 18849298
references: Arthritis Rheum. 2006 Mar;54(3):832-42
references: 16508964
references: J Histochem Cytochem. 2004 Sep;52(9):1151-7
references: 15314082
references: J Cell Biochem. 2009 Nov 1;108(4):839-50
references: 19693768
references: Tissue Eng. 2003 Jun;9(3):431-9
references: 12857411
references: Hepatology. 2004 Dec;40(6):1275-84
references: 15562440
references: Tissue Eng. 2006 Feb;12(2):369-79
references: 16548695
references: Chin Med J (Engl). 2011 Feb;124(4):606-10
references: 21362289
references: Clin Sports Med. 2003 Oct;22(4):675-92
references: 14560540
references: Aging Cell. 2010 Oct;9(5):911-5
references: 20569237
references: J Cell Biochem. 1997 Feb;64(2):278-94
references: 9027588
references: Am J Sports Med. 1999 Jul-Aug;27(4):476-88
references: 10424218
references: Injury. 2005 Nov;36(11):1267-72
references: 16214470
references: J Orthop Res. 2010 Feb;28(2):198-203
references: 19688869
references: Stem Cells. 2007 Jun;25(6):1384-92
references: 17332507
references: Histochem Cell Biol. 2009 Jun;131(6):733-41
references: 19280209
references: Am J Sports Med. 2010 Dec;38(12):2477-86
references: 20802092
references: J Clin Invest. 1999 Mar;103(5):697-705
references: 10074487
references: J Orthop Surg Res. 2009 Jul 21;4:27
references: 19622147
references: J Hand Surg Am. 2007 May-Jun;32(5):597-605
references: 17481995
references: Stem Cells. 2004;22(5):675-82
references: 15342932
references: J Biomech. 2008;41(5):1095-103
references: 18191137
references: Rheumatology (Oxford). 2010 Feb;49(2):231-9
references: 19955224
references: J Orthop Res. 2003 May;21(3):420-31
references: 12706014
references: Rheumatology (Oxford). 2004 Feb;43(2):131-42
references: 12867575
references: J Orthop Res. 1998 Sep;16(5):618-21
references: 9820287
references: BMC Cell Biol. 2006 Mar 10;7:14
references: 16529651
references: J Orthop Res. 2010 May;28(5):639-43
references: 19918904
references: Cell Stem Cell. 2008 Sep 11;3(3):301-13
references: 18786417
references: J Musculoskelet Neuronal Interact. 2002 Sep;2(5):440-7
references: 15758412
references: Cell Tissue Res. 2007 Mar;327(3):449-62
references: 17053900
references: J Bone Joint Surg Am. 1997 Jul;79(7):997-1006
references: 9234875
references: Science. 1999 Apr 2;284(5411):143-7
references: 10102814
references: Stem Cells. 2009 Jun;27(6):1276-87
references: 19489094
references: Clin Orthop Relat Res. 2002 Jul;(400):174-83
references: 12072760
references: Exp Cell Res. 2003 Jul 15;287(2):289-300
references: 12837285
references: J Orthop Res. 2004 Sep;22(5):998-1003
references: 15304271
references: Mol Cell Biol. 2004 Apr;24(8):3460-72
references: 15060165
references: Arthritis Res Ther. 2006;8(4):R92
references: 16719933
references: Am J Sports Med. 2006 Nov;34(11):1801-8
references: 16816149
references: J Orthop Res. 1998 Jul;16(4):406-13
references: 9747780
references: Arterioscler Thromb Vasc Biol. 2007 Apr;27(4):776-82
references: 17272757
references: Acta Orthop. 2005 Jun;76(3):421-7
references: 16156473
references: Nat Med. 2007 Oct;13(10):1219-27
references: 17828274
references: J Bone Joint Surg Am. 2002 Jul;84(7):1123-31
references: 12107310
references: Tissue Eng Part A. 2009 Aug;15(8):2259-66
references: 19260778
references: Ann Rheum Dis. 1996 Feb;55(2):109-15
references: 8712860
references: Cell Transplant. 2009;18(9):1013-28
references: 19523328
references: Am J Sports Med. 2007 Apr;35(4):597-604
references: 17218656
references: Circ Res. 2009 Mar 27;104(6):733-41
references: 19197075
references: Bone. 2009 Oct;45(4):726-35
references: 19540374
references: J Orthop Res. 2006 Feb;24(2):173-82
references: 16435354
references: Calcif Tissue Int. 2009 Jul;85(1):55-65
references: 19424738
references: Tissue Eng Part A. 2010 May;16(5):1621-32
references: 20001220
references: Cytotechnology. 2009 Nov;61(1-2):1-10
references: 19842053
references: J Orthop Res. 2010 Sep;28(9):1178-83
references: 20225313
references: Eur J Cell Biol. 2009 May;88(5):257-71
references: 19303661
references: Int J Exp Pathol. 2007 Aug;88(4):227-35
references: 17696903
references: Proc Natl Acad Sci U S A. 1999 Sep 14;96(19):10711-6
references: 10485891
references: Front Biosci (Elite Ed). 2010 Jan 01;2:1-12
references: 20036847
references: J Cell Biochem. 2006 Dec 1;99(5):1285-97
references: 16795045
references: Connect Tissue Res. 2003;44(3-4):128-33
references: 14504032
references: J Anat. 1996 Dec;189 ( Pt 3):593-600
references: 8982835
references: BMC Musculoskelet Disord. 2010 Jan 18;11:10
references: 20082706
references: Exp Hematol. 2000 Jun;28(6):707-15
references: 10880757
references: Leukemia. 2003 Jan;17(1):160-70
references: 12529674
references: Rheumatology (Oxford). 2002 Apr;41(4):474-6
references: 11961186
references: Tissue Eng. 2005 Mar-Apr;11(3-4):448-57
references: 15869423
references: J Histochem Cytochem. 2009 Feb;57(2):91-100
references: 18824634
references: Med Sci Sports Exerc. 1993 May;25(5):603-7
references: 8388071
references: Curr Opin Biotechnol. 2009 Oct;20(5):537-44
references: 19879127
references: Peptides. 1997;18(2):323-6
references: 9149307
references: Calcif Tissue Int. 2008 Mar;82(3):238-47
references: 18305886
references: Clin Orthop Relat Res. 2008 Jul;466(7):1528-38
references: 18478310
references: J Clin Invest. 2006 Apr;116(4):940-52
references: 16585960
references: J Orthop Res. 2011 Mar;29(3):390-6
references: 20882582
references: J Cell Sci. 2006 Jun 1;119(Pt 11):2204-13
references: 16684817
references: Acta Orthop Scand. 2002 Dec;73(6):658-62
references: 12553513
references: J Cell Sci. 2000 Apr;113 ( Pt 7):1161-6
references: 10704367
references: J Cell Biochem. 2003 Jun 1;89(3):556-62
references: 12761889
references: J Orthop Res. 2007 Nov;25(11):1415-24
references: 17557323
references: Ann Rheum Dis. 2005 Jul;64(7):1083-6
references: 15958764
references: Cell Stem Cell. 2008 Sep 11;3(3):229-30
references: 18786406
references: Eur Spine J. 2007 Aug;16(8):1075-84
references: 17426989
references: J Bone Miner Res. 2001 Jun;16(6):1120-9
references: 11393789
references: Tissue Eng Part A. 2010 Jul;16(7):2237-53
references: 20163211
references: J Orthop Res. 2011 Jun;29(6):816-21
references: 21520255
references: J Orthop Res. 2007 May;25(5):617-24
references: 17318892
references: J Neurosurg Spine. 2007 Aug;7(2):174-83
references: 17688057
references: Am J Sports Med. 2008 Mar;36(3):461-73
references: 18216273