title: Increased expression and phosphorylation of sh-ptp2 (syp) in human gastric carcinomas.
abstract: The expression of SH-PTP2 (SYP), a novel protein tyrosine phosphatase with src homology 2, was examined in human gastric carcinoma cell lines and gastric carcinoma tissues as well as corresponding non-neoplastic mucosas by Northern and Western blotting. The expression of SH-PTP2 mRNA was detected in all 8 gastric carcinoma cell lines at various levels. Most of the cell lines expressed SH-PTP2 protein of 70 kd which corresponds to tyrosine-phosphorylated form, whereas only one cell line expressed unphosphorylated form of SH-PTP2; Fourteen (66.6%) of 21 gastric carcinomas expressed SH-PTP2 mRNA at higher level than non-neoplastic mucosas. Furthermore, most of the gastric carcinomas displayed higher levels of tyrosine-phosphorylated form of SH-PTP2, while non-neoplastic mucosas tended to express unphosphorylated SH-PTP2 protein. These results suggest that the increased expression and tyrosine-phosphorylation of SH-PTP2 may participate in stomach carcinogenesis.
authors: HIROSHIMA UNIV,SCH MED,DEPT PATHOL 1,MINAMI KU,HIROSHIMA 734,JAPAN. SAGA MED SCH,DEPT SURG,SAGA 849,JAPAN.
authors: K
authors: K
authors: Kitahara
authors: W
authors: W
authors: Yasui
authors: H
authors: H
authors: Kuniyasu
authors: H
authors: H
authors: Yokozaki
authors: S
authors: S
authors: Yunotani
authors: T
authors: T
authors: Hisatsugu
authors: E
authors: E
authors: Tahara