title: Application of hydrophobic palladium nanoparticles for the development of electrochemical glucose biosensor.
abstract: An amperometric glucose biosensor based on an n-alkylamine-stabilized palladium nanoparticles (PdNPs)-glucose oxidase (GOx) modified glassy carbon (GC) electrode has been successfully fabricated. PdNPs were initially synthesized by a biphase mixture of water and toluene method using n-alkylamines (dodecylamine, C₁₂-NH₂ and octadecylamine, C₁₈-NH₂) as stabilizing ligands. The performance of the PdNPs-GOx/GC biosensor was studied by cyclic voltammetry. The optimum working potential for amperometric measurement of glucose in pH 7.0 phosphate buffer solution is -0.02 V (vs. Ag/AgCl). The analytical performance of the biosensor prepared from C₁₈-PdNPs-GOx is better than that of C₁₂-PdNPs-GOx. The C₁₈-PdNPs-GOx/GC biosensor exhibits a fast response time of ca. 3s, a detection limit of 3.0 μM (S/N=3) and a linear range of 3.0 μM-8.0 mM. The linear dependence of current density with glucose concentration is 70.8 μA cm⁻² mM⁻¹. The biosensor shows good stability, repeatability and reproducibility. It has been successfully applied to determine the glucose content in human blood serum samples.
authors: Research Center of Environmental Science and Engineering, Shanxi University, Taiyuan 030006, PR China.
authors: Zhongping
authors: Z
authors: Li
authors: Xu
authors: X
authors: Wang
authors: Guangming
authors: G
authors: Wen
authors: Shaomin
authors: S
authors: Shuang
authors: Chuan
authors: C
authors: Dong
authors: Man Chin
authors: MC
authors: Paau
authors: Martin M F
authors: MM
authors: Choi