title: Semaphorin 3A induces CaV2.3 channel-dependent conversion of axons to dendrites.
abstract: Polarized neurites (axons and dendrites) form the functional circuitry of the nervous system. Secreted guidance cues often control the polarity of neuron migration and neurite outgrowth by regulating ion channels. Here, we show that secreted semaphorin 3A (Sema3A) induces the neurite identity of Xenopus spinal commissural interneurons (xSCINs) by activating Ca(V)2.3 channels (Ca(V)2.3). Sema3A treatment converted the identity of axons of cultured xSCINs to that of dendrites by recruiting functional Ca(V)2.3. Inhibition of Sema3A signalling prevented both the expression of Ca(V)2.3 and acquisition of the dendrite identity, and inhibition of Ca(V)2.3 function resulted in multiple axon-like neurites of xSCINs in the spinal cord. Furthermore, Sema3A-triggered cGMP production and PKG activity induced, respectively, the expression of functional Ca(V)2.3 and the dendrite identity. These results reveal a mechanism by which a guidance cue controls the identity of neurites during nervous system development.
authors: Department of Biochemistry, New York University School of Medicine, New York, New York 10016-6402, USA. nishim01@nyumc.org
authors: Makoto
authors: M
authors: Nishiyama
authors: Kazunobu
authors: K
authors: Togashi
authors: Melanie J
authors: MJ
authors: von Schimmelmann
authors: Chae-Seok
authors: CS
authors: Lim
authors: Shin-ichi
authors: S
authors: Maeda
authors: Naoya
authors: N
authors: Yamashita
authors: Yoshio
authors: Y
authors: Goshima
authors: Shin
authors: S
authors: Ishii
authors: Kyonsoo
authors: K
authors: Hong
references: Neuroscientist. 2011 Feb;17(1):54-78
references: 20431046
references: Neural Dev. 2007 Sep 10;2:17
references: 17845723
references: J Neurophysiol. 2001 Jul;86(1):269-79
references: 11431508
references: Cell. 2007 Apr 20;129(2):385-95
references: 17448996
references: Cell. 2005 Jan 14;120(1):123-35
references: 15652487
references: Neuron. 2008 Jun 12;58(5):694-707
references: 18549782
references: Nature. 2000 Apr 6;404(6778):567-73
references: 10766232
references: J Neurosci. 1993 Nov;13(11):4936-48
references: 8229206
references: J Neurosci. 2006 Mar 15;26(11):2971-80
references: 16540575
references: Nature. 2000 Nov 30;408(6812):589-93
references: 11117746
references: Annu Rev Neurosci. 1994;17:267-310
references: 8210176
references: Nat Neurosci. 2005 Feb;8(2):179-86
references: 15643427
references: Neuron. 2003 Mar 27;37(6):939-52
references: 12670423
references: Science. 2001 Aug 3;293(5531):872-5
references: 11486090
references: Neuron. 2001 Dec 20;32(6):1013-26
references: 11754834
references: Nature. 2008 Oct 2;455(7213):669-73
references: 18776887
references: Nat Rev Neurosci. 2007 Mar;8(3):194-205
references: 17311006
references: Annu Rev Neurosci. 2009;32:347-81
references: 19400726
references: Curr Biol. 2008 Jul 8;18(13):992-1000
references: 18595703
references: Development. 1988 Jul;103(3):447-61
references: 3246217
references: Dev Biol. 2005 Dec 15;288(2):474-86
references: 16293241
references: Trends Cell Biol. 2004 Jun;14(6):320-30
references: 15183189
references: Cell. 2005 Jan 14;120(1):137-49
references: 15652488
references: Science. 1998 Sep 4;281(5382):1515-8
references: 9727979
references: Cell. 2010 Jul 9;142(1):144-57
references: 20603020
references: Curr Biol. 2000 May 4;10(9):555-8
references: 10801446
references: Nature. 2003 Jun 26;423(6943):990-5
references: 12827203
references: Curr Biol. 2000 Nov 16;10(22):1467-70
references: 11102812
references: Nature. 2000 Jan 6;403(6765):93-8
references: 10638760
references: FEBS Lett. 2002 Oct 23;530(1-3):209-14
references: 12387894
references: Neuroscientist. 2010 Feb;16(1):65-78
references: 19700742
references: Nat Neurosci. 2008 Jul;11(7):762-71
references: 18536712
references: Genes Cells. 2005 Feb;10(2):165-79
references: 15676027
references: Science. 2001 Mar 9;291(5510):1928-38
references: 11239147
references: J Neurosci. 1999 Aug 1;19(15):6417-26
references: 10414970
references: Eur J Neurosci. 2003 Oct;18(8):2175-87
references: 14622178
references: J Neurobiol. 1997 Sep;33(3):316-28
references: 9298768
references: Neuron. 2003 Oct 9;40(2):229-42
references: 14556706
references: Cell. 2009 Mar 20;136(6):1148-60
references: 19268344
references: Nature. 2009 Dec 24;462(7276):1065-9
references: 20010807
references: Nat Med. 2006 Dec;12(12):1380-9
references: 17099709
references: J Neurobiol. 2004 Feb 15;58(3):403-12
references: 14750152
references: Science. 2010 Jan 29;327(5965):547-52
references: 20110498
references: Nat Neurosci. 2006 Apr;9(4):511-8
references: 16520734
references: Cell. 2007 May 4;129(3):565-77
references: 17482549
references: J Comp Neurol. 1987 Jul 15;261(3):435-49
references: 3611420
references: J Physiol. 1995 Aug 15;487(1):67-90
references: 7473260
references: Cell. 2007 May 4;129(3):549-63
references: 17482548