title: Dietary n-3 fatty acids significantly suppress lipogenesis in bovine muscle and adipose tissue: a functional genomics approach.
abstract: Changes in fatty acid composition of longissimus muscle and subcutaneous adipose tissue of German Holstein bulls induced by a grass-silage/n-3 fatty acid based intervention diet versus a maize-silage/n-6 fatty acid based control diet were analyzed and related to shifts in lipogenic gene expression, protein expression, and enzyme activity patterns. Significantly higher amounts of n-3 fatty acids and by mean factors of 2.2-2.5 decreased n-6/n-3 fatty acid ratios in both tissues were obtained upon n-3 fatty acid intervention. In longissimus muscle, these changes of fatty acid profiles were associated with reduced SREBP1c (p = 0.02), ACC (p = 0.00), FAS (p = 0.10) and SCD (p = 0.03) gene expression, Δ6D (p = 0.03) and SCD (p = 0.03) protein expression as well as SCD enzyme activity (p = 0.03). In subcutaneous adipose tissue, significantly reduced ACC (p = 0.00) and FAS (p = 0.01) gene expression, SCD protein expression (p = 0.02) and SCD enzyme activity (p = 0.03) were detected upon n-3 fatty acid intervention, although lower degrees of correlation between gene and corresponding gene products were obtained in relation to longissimus muscle. The study elucidates tissue-specific functional genomic responses to dietary fatty acid manipulation in regard to fatty acid profile tailoring of animal tissues.
authors: Research Unit Muscle Biology and Growth, Leibniz Institute for Farm Animal Biology, Wilhelm-Stahl-Allee 2, 18196 Dummerstorf, Germany. hiller@fbn-dummerstorf.de
authors: Beate
authors: B
authors: Hiller
authors: Andrea
authors: A
authors: Herdmann
authors: Karin
authors: K
authors: Nuernberg
references: Adv Exp Med Biol. 2008;606:67-108
references: 18183925
references: J Anim Sci. 2004 Mar;82(3):747-58
references: 15032431
references: J Anim Sci. 2009 Jan;87(1):119-30
references: 18820161
references: Am J Clin Nutr. 2009 Dec;90(6):1656-64
references: 19828712
references: J Anim Sci. 2009 Dec;87(12):3905-14
references: 19684259
references: J Biol Chem. 1957 May;226(1):497-509
references: 13428781
references: J Anim Sci. 2009 Apr;87(14 Suppl):E72-82
references: 18997081
references: Prog Lipid Res. 2009 Sep;48(5):257-74
references: 19409927
references: Meat Sci. 2004 Jan;66(1):21-32
references: 22063928
references: J Agric Food Chem. 2004 Oct 20;52(21):6607-15
references: 15479030
references: Bioinform Biol Insights. 2010 Mar 31;4:19-31
references: 20448844
references: BMB Rep. 2008 Dec 31;41(12):846-51
references: 19123974
references: Lipids. 2010 Nov;45(11):963-73
references: 20886305
references: Animal. 2010 May;4(5):755-62
references: 22444129
references: Br J Nutr. 2006 Mar;95(3):609-17
references: 16512947
references: J Physiol Pharmacol. 2006 Nov;57 Suppl 10:31-42
references: 17242489
references: J Anim Sci. 2009 Jan;87(1):244-52
references: 18791145
references: Meat Sci. 2006 Sep;74(1):17-33
references: 22062713
references: Comp Biochem Physiol A Mol Integr Physiol. 2007 Nov;148(3):629-34
references: 17851104
references: J Anim Sci. 2007 Jul;85(7):1731-40
references: 17431049
references: Nucleic Acids Res. 2002 May 1;30(9):e36
references: 11972351
references: J Anim Sci. 2007 Nov;85(11):2882-94
references: 17591707
references: Nutrition. 2006 Jan;22(1):47-53
references: 16289978
references: J Agric Food Chem. 2010 Jul 28;58(14):8407-13
references: 20583808
references: J Anim Sci. 2011 Jan;89(1):12-22
references: 20852082
references: Am J Clin Nutr. 2006 Jun;83(6 Suppl):1499S-1504S
references: 16841860
references: Meat Sci. 2000 Jun;55(2):201-12
references: 22061086
references: J Biol Chem. 2007 Jan 26;282(4):2483-93
references: 17127673
references: Domest Anim Endocrinol. 2004 Nov;27(4):303-31
references: 15519037
references: Anim Genet. 2010 Aug;41(4):417-20
references: 20002363
references: J Anim Sci. 2007 Feb;85(2):380-7
references: 17235023
references: Meat Sci. 2006 Sep;74(1):209-18
references: 22062730
references: Meat Sci. 2009 Apr;81(4):738-44
references: 20416561
references: Meat Sci. 2010 May;85(1):15-20
references: 20374858
references: J Nutr. 2000 Dec;130(12):3110S-3115S
references: 11110881
references: J Agric Food Chem. 2007 Nov 14;55(23):9698-703
references: 17935292
references: Meat Sci. 2008 Apr;78(4):343-58
references: 22062452
references: J Agric Food Chem. 2010 Jul 28;58(14):8314-21
references: 20597504
references: BMC Vet Res. 2010 Jul 22;6:40
references: 20649987
references: Am J Clin Nutr. 2006 Jun;83(6 Suppl):1526S-1535S
references: 16841863
references: Meat Sci. 2011 Apr;87(4):344-8
references: 21145173
references: Meat Sci. 2010 Oct;86(2):440-50
references: 20573458
references: Meat Sci. 2009 Feb;81(2):396-404
references: 22064180
references: Meat Sci. 2009 Jul;82(3):357-64
references: 20416712
references: Anim Genet. 2008 Feb;39(1):62-70
references: 18254736
references: J Endocrinol. 2007 Jan;192(1):229-36
references: 17210760