title: Development of intron targeting (IT) markers for potato and cross-species amplification in Solanum nigrum (Solanaceae).
abstract: OBJECTIVE
  Intron Targeting (IT) primers were developed for potato using expressed sequence tags (EST) and NCBI database records to study genetic diversity. •
  
  
  RESULTS
  Twenty-nine polymorphic intron targeting (IT) markers were generated and characterized from 30 samples of potato and 22 samples of Solanum nigrum to detect polymorphism. The number of alleles (A) per locus ranged from 2 to 7 in the analyzed populations, and the observed heterozygosity (H(O)) and expected heterozygosity (H(E)) from 0 to 0.833 and 0.750, respectively. All of the primers also amplified in the related species S. nigrum. •
  
  
  CONCLUSIONS
  The developed markers will provide valuable tools for genetic diversity analysis, genetic mapping, and marker-assisted breeding of potato and related Solanum species.
authors: Plant Biology Division, Department of Biosciences, University of Helsinki, FIN-00014 PO Box 65, Helsinki, Finland.
authors: Péter
authors: P
authors: Poczai
authors: István
authors: I
authors: Cernák
authors: Ahmad Mosapour
authors: AM
authors: Gorji
authors: Sándor
authors: S
authors: Nagy
authors: János
authors: J
authors: Taller
authors: Zsolt
authors: Z
authors: Polgár