title: Construction of a Der p2-transgenic plant for the alleviation of airway inflammation.
abstract: In clinical therapy, the amount of antigen administered to achieve oral tolerance for allergic diseases is large, and the cost is a major consideration. In this study, we used tobacco plants to develop a large-scale protein production system for allergen-specific immunotherapy, and we investigated the mechanisms of oral tolerance induced by a transgenic plant-derived antigen. We used plants (tobacco leaves) transgenic for the Dermatophagoides pteronyssinus 2 (Der p2) antigen to produce Der p2. Mice received total protein extract from Der p2 orally once per day over 6 days (days 0-2 and days 6-8). Mice were also sensitized and challenged with yeast-derived recombinant Der p2 (rDer p2), after which the mice were examined for airway hyper-responsiveness and airway inflammation. After sensitization and challenge with rDer p2, mice that were fed with total protein extracted from transgenic plants showed decreases in serum Der p2-specific IgE and IgG1 titers, decreased IL-5 and eotaxin levels in bronchial alveolar lavage fluid, and eosinophil infiltration in the airway. In addition, hyper-responsiveness was also decreased in mice that were fed with total protein extracted from transgenic plants, and CD4(+)CD25(+)Foxp3(+) regulatory T cells were significantly increased in mediastinal and mesenteric lymph nodes. Furthermore, splenocytes isolated from transgenic plant protein-fed mice exhibited decreased proliferation and increased IL-10 secretion after stimulation with rDer p2. The data here suggest that allergen-expressing transgenic plants could be used for therapeutic purposes for allergic diseases.
authors: Department of Microbiology and Immunology, School of Medicine, China Medical University, Taichung, Taiwan.
authors: C C
authors: CC
authors: Lee
authors: H
authors: H
authors: Ho
authors: K T
authors: KT
authors: Lee
authors: S T
authors: ST
authors: Jeng
authors: B L
authors: BL
authors: Chiang
references: J Allergy Clin Immunol. 2003 Mar;111(3):437-48; quiz 449
references: 12642818
references: Nat Rev Immunol. 2010 Mar;10(3):170-81
references: 20154735
references: Nature. 1995 Jul 13;376(6536):177-80
references: 7603570
references: Immunity. 2008 Jul 18;29(1):114-26
references: 18617425
references: J Allergy Clin Immunol. 2002 May;109(5 Suppl):S482-9
references: 11994720
references: J Exp Med. 1993 Sep 1;178(3):889-99
references: 8102390
references: Curr Opin Allergy Clin Immunol. 2003 Apr;3(2):139-45
references: 12750611
references: Clin Exp Allergy. 2003 Feb;33(2):206-10
references: 12580913
references: Immunol Today. 1992 Dec;13(12):501-7
references: 1361126
references: J Allergy Clin Immunol. 1999 Aug;104(2 Pt 1):425-32
references: 10452766
references: Nature. 1987 Feb 12-18;325(6105):622-5
references: 3492677
references: Annu Rev Immunol. 2001;19:683-765
references: 11244051
references: J Allergy Clin Immunol. 1998 Jun;101(6 Pt 1):747-54
references: 9648701
references: Nat Med. 1997 Jul;3(7):793-6
references: 9212110
references: J Appl Physiol (1985). 2004 Sep;97(3):1104-11
references: 15121749
references: Clin Exp Allergy. 1994 Jan;24(1):53-9
references: 8156447
references: J Clin Invest. 1995 Sep;96(3):1303-10
references: 7657805
references: J Allergy Clin Immunol. 2003 Feb;111(2 Suppl):S712-9
references: 12592316
references: N Engl J Med. 1992 Jan 30;326(5):298-304
references: 1530827
references: JAMA. 1992 Nov 25;268(20):2834-9
references: 1433698
references: Am J Respir Crit Care Med. 1997 Sep;156(3 Pt 1):766-75
references: 9309991
references: Nat Rev Immunol. 2008 Jul;8(7):523-32
references: 18566595
references: J Microbiol Immunol Infect. 2002 Sep;35(3):152-8
references: 12380787
references: Trends Microbiol. 1998 Nov;6(11):449-53
references: 9846363
references: Trends Plant Sci. 2001 May;6(5):219-26
references: 11335175
references: J Immunol. 1995 May 1;154(9):4390-4
references: 7722296
references: Proc Natl Acad Sci U S A. 1991 Feb 1;88(3):834-8
references: 1992474
references: J Allergy Clin Immunol. 2011 Mar;127(3):696-700
references: 21377039
references: Clin Exp Allergy. 1999 Sep;29(9):1164-71
references: 10469024
references: Int Immunol. 1996 Mar;8(3):335-42
references: 8671619
references: Allergy. 1997 Nov;52(11):1115-9
references: 9404565
references: Immunol Rev. 2005 Aug;206:232-59
references: 16048553
references: J Immunol. 2001 Oct 15;167(8):4245-53
references: 11591746
references: J Immunol. 1996 Aug 15;157(4):1337-41
references: 8759712