title: Simultaneous production and characterization of medium-chain-length polyhydroxyalkanoates and alginate oligosaccharides by Pseudomonas mendocina NK-01.
abstract: When Pseudomonas mendocina NK-01 was cultivated in a 200-L fermentor using glucose as carbon source, 0.316 g L(-1) medium-chain-length polyhydroxyalkanoate (PHA(MCL)) and 0.57 g L(-1) alginate oligosaccharides (AO) were obtained at the end of the process. GC/MS was used to characterize the PHA(MCL), which was found to be a polymer mainly consisting of 3HO (3-hydroxyoctanoate) and 3HD (3-hydroxydecanoate). T (m) and T (g) values for the PHA(MCL) were 51.03 °C and -41.21 °C, respectively, by DSC. Its decomposition temperature was about 300 °C. The elongation at break was 700% under 12 MPa stress. MS and GPC were also carried out to characterize the AO which had weight-average molecular weights of 1,546 and 1,029 Da, respectively, for the two main components at the end of the fermentation process. MS analysis revealed that the AO were consisted of β-D-mannuronic acid and/or α-L-guluronic acid, and the β-D-mannuronic acid and/or α-L-guluronic acid residues were partially acetylated at position C2 or C3.
authors: Key Laboratory of Molecular MicroBiology and Technology, Ministry of Education, Department of MicroBiology, College of Life Sciences, Nankai University, Tianjin 300071, China.
authors: Wenbin
authors: W
authors: Guo
authors: Cunjiang
authors: C
authors: Song
authors: Meimei
authors: M
authors: Kong
authors: Weitao
authors: W
authors: Geng
authors: Yuanyuan
authors: Y
authors: Wang
authors: Shufang
authors: S
authors: Wang