title: Functional impairment of central memory CD4 T cells is a potential early prognostic marker for changing viral load in SHIV-infected rhesus macaques.
abstract: In HIV infection there is a paucity of literature about the degree of immune dysfunction to potentially correlate and/or predict disease progression relative to CD4(+) T cells count or viral load. We assessed functional characteristics of memory T cells subsets as potential prognostic markers for changing viral loads and/or disease progression using the SHIV-infected rhesus macaque model. Relative to long-term non-progressors with low/undetectable viral loads, those with chronic plasma viremia, but clinically healthy, exhibited significantly lower numbers and functional impairment of CD4(+) T cells, but not CD8(+) T cells, in terms of IL-2 production by central memory subset in response to PMA and ionomycine (PMA+I) stimulation. Highly viremic animals showed impaired cytokine-production by all T cells subsets. These results suggest that functional impairment of CD4(+) T cells in general, and of central memory subset in particular, may be a potential indicator/predictor of chronic infection with immune dysfunction, which could be assayed relatively easily using non-specific PMA+I stimulation.
authors: Department of Immunology, The University of Texas M.D. Anderson Cancer Center, Houston, Texas, United States of America.
authors: Hong
authors: H
authors: He
authors: Pramod N
authors: PN
authors: Nehete
authors: Bharti
authors: B
authors: Nehete
authors: Eric
authors: E
authors: Wieder
authors: Guojun
authors: G
authors: Yang
authors: Stephanie
authors: S
authors: Buchl
authors: K Jagannadha
authors: KJ
authors: Sastry
references: N Engl J Med. 2009 Apr 30;360(18):1815-26
references: 19339714
references: J Clin Invest. 1997 Apr 1;99(7):1739-50
references: 9120019
references: Nature. 1995 Jan 12;373(6510):123-6
references: 7816094
references: Science. 2002 Jun 28;296(5577):2325-6
references: 12089425
references: Nature. 2006 Jun 15;441(7095):890-3
references: 16778891
references: J Immunol. 2002 Jan 1;168(1):29-43
references: 11751943
references: J Exp Med. 2006 Oct 2;203(10):2281-92
references: 16954372
references: Microbiol Rev. 1993 Mar;57(1):183-289
references: 8464405
references: J Exp Med. 1998 Dec 21;188(12):2199-204
references: 9858506
references: Eur J Immunol. 2007 Jun;37(6):1502-12
references: 17492805
references: Science. 1997 Nov 21;278(5342):1447-50
references: 9367954
references: J Immunol. 1998 Nov 15;161(10):5284-95
references: 9820501
references: Annu Rev Immunol. 2004;22:745-63
references: 15032595
references: Clin Immunol. 2004 Mar;110(3):199-205
references: 15047198
references: Science. 1999 Nov 12;286(5443):1353-7
references: 10558989
references: Virology. 2008 Jan 5;370(1):130-41
references: 17920095
references: JAMA. 2006 Sep 27;296(12):1498-506
references: 17003398
references: N Engl J Med. 2006 Nov 30;355(22):2283-96
references: 17135583
references: J Exp Med. 2007 Sep 3;204(9):2171-85
references: 17724130
references: Vaccine. 2005 Mar 18;23(17-18):2154-9
references: 15755586
references: J Virol. 2007 Jan;81(2):893-902
references: 17093193
references: J Clin Invest. 1995 May;95(5):2061-6
references: 7738173
references: Nat Med. 2002 Mar;8(3):207-10
references: 11875482
references: AIDS Res Hum Retroviruses. 1998 Jan 20;14(2):183-9
references: 9462929
references: J Immunol Methods. 2000 Sep 21;243(1-2):107-24
references: 10986410
references: J Immunol. 2006 May 1;176(9):5338-45
references: 16622001
references: AIDS. 2004 Jan 2;18(1):13-23
references: 15090825
references: Nat Med. 1999 May;5(5):518-25
references: 10229228
references: Lancet. 2009 Apr 18;373(9672):1352-63
references: 19361855
references: Science. 2006 Jun 9;312(5779):1530-3
references: 16763152