title: A V-shaped cationic dye for nonlinear optical bioimaging.
abstract: A symmetric cationic molecule with D-π-A(+)-π-D architecture was synthesized with high two-photon absorption cross-section (σ(2) ≈ 1140 GM). Application as a marker in fluorescence microscopy of living cells revealed its presence inside the cell staining vesicular shape organelles in the cytoplasm. Fluorescence lifetime imaging microscopy shows that it is also able to penetrate within the nucleus.
authors: Centro de Química-Física Molecular (CQFM) and Institute of Nanoscience and Nanotechnology (IN), Instituto Superior Técnico, 1049-001 Lisboa, Portugal. ermelinda.macoas@ist.utl.pt
authors: Ermelinda
authors: E
authors: Maçôas
authors: Gema
authors: G
authors: Marcelo
authors: Sandra
authors: S
authors: Pinto
authors: Tatiana
authors: T
authors: Cañeque
authors: Ana M
authors: AM
authors: Cuadro
authors: Juan J
authors: JJ
authors: Vaquero
authors: José M G
authors: JM
authors: Martinho