title: Enoyl-coenzyme A hydratase and antigen 85B of Mycobacterium habana are specifically recognized by antibodies in sera from leprosy patients.
abstract: Leprosy is an infectious disease caused by Mycobacterium leprae, which is a noncultivable bacterium. One of the principal goals of leprosy research is to develop serological tests that will allow identification and early treatment of leprosy patients. M. habana is a cultivable nonpathogenic mycobacterium and candidate vaccine for leprosy, and several antigens that cross-react between M. leprae and M. habana have been discovered. The aim of the present study was to extend the identification of cross-reactive antigens by identifying M. habana proteins that reacted by immunoblotting with antibodies in serum samples from leprosy patients but not with antibodies in sera from tuberculosis (TB) patients or healthy donors (HDs). A 28-kDa antigen that specifically reacted with sera from leprosy patients was identified. To further characterize this antigen, protein spots were aligned in two-dimensional polyacrylamide gels and Western blots. Spots cut out from the gels were then analyzed by mass spectrometry. Two proteins were identified: enoyl-coenzyme A hydratase (lipid metabolism; ML2498) and antigen 85B (Ag85B; mycolyltransferase; ML2028). These proteins represent promising candidates for the design of a reliable tool for the serodiagnosis of lepromatous leprosy, which is the most frequent form in Mexico.
authors: Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, México, D.F., México.
authors: J
authors: J
authors: Serafín-López
authors: M
authors: M
authors: Talavera-Paulin
authors: J C
authors: JC
authors: Amador-Molina
authors: M
authors: M
authors: Alvarado-Riverón
authors: M M
authors: MM
authors: Vilchis-Landeros
authors: P
authors: P
authors: Méndez-Ortega
authors: M
authors: M
authors: Fafutis-Morris
authors: V
authors: V
authors: Paredes-Cervantes
authors: R
authors: R
authors: López-Santiago
authors: C I
authors: CI
authors: León
authors: M I
authors: MI
authors: Guerrero
authors: R M
authors: RM
authors: Ribas-Aparicio
authors: G
authors: G
authors: Mendoza-Hernández
authors: C
authors: C
authors: Carreño-Martínez
authors: S
authors: S
authors: Estrada-Parra
authors: I
authors: I
authors: Estrada-García
references: J Proteomics. 2011 Aug 24;74(9):1711-9
references: 21278007
references: Am Rev Respir Dis. 1975 Feb;111(2):196-200
references: 1167440
references: Biochim Biophys Acta. 2007 Feb;1774(2):303-11
references: 17240207
references: FEMS Immunol Med Microbiol. 1999 Nov;26(2):143-51
references: 10536301
references: Indian J Exp Biol. 1979 Nov;17(11):1190-3
references: 549844
references: Infect Immun. 1995 Jan;63(1):88-93
references: 7806388
references: Indian J Exp Biol. 1994 Jul;32(7):478-81
references: 7959924
references: Am J Trop Med Hyg. 1998 Feb;58(2):133-6
references: 9502593
references: Int J Lepr Other Mycobact Dis. 1980 Dec;48(4):371-81
references: 7009447
references: Scand J Immunol. 2000 May;51(5):502-10
references: 10792843
references: Vaccine. 1999 Dec 10;18(9-10):795-8
references: 10580191
references: Clin Vaccine Immunol. 2011 Feb;18(2):260-7
references: 21177913
references: Indian J Lepr. 1988 Oct;60(4):593-9
references: 3075637
references: Infect Immun. 2006 Jan;74(1):175-82
references: 16368971
references: Lepr Rev. 1995 Mar;66(1):31-8
references: 7731339
references: J Immunol Methods. 1991 Dec 15;145(1-2):223-8
references: 1765655
references: Int J Tuberc Lung Dis. 1999 Dec;3(12):1106-12
references: 10599015
references: Nature. 1970 Aug 15;227(5259):680-5
references: 5432063
references: Infect Immun. 1983 Jun;40(3):1096-103
references: 6343239
references: Rev Soc Bras Med Trop. 2008;41 Suppl 2:11-8
references: 19618069
references: Clin Exp Immunol. 1994 Apr;96(1):86-90
references: 8149672
references: Infect Immun. 1989 Feb;57(2):653-5
references: 2643581
references: Anal Chem. 1999 Nov 1;71(21):4800-7
references: 10565271
references: FEMS Immunol Med Microbiol. 2010 Feb;58(1):128-37
references: 19878316
references: Int J Lepr Other Mycobact Dis. 1990 Dec;58(4):641-50
references: 2280114
references: Int J Lepr Other Mycobact Dis. 1966 Jul-Sep;34(3):255-73
references: 5950347
references: Clin Microbiol Rev. 2005 Jan;18(1):81-101
references: 15653820
references: Microbiology (Reading). 1996 Jun;142 ( Pt 6):1375-1383
references: 8704977
references: Proteomics. 2007 Sep;7(18):3332-41
references: 17849409
references: Int J Lepr Other Mycobact Dis. 1999 Jun;67(2):168-70
references: 10472373
references: J Immunol. 1990 Mar 1;144(5):1922-5
references: 2407785
references: Infect Immun. 1990 Jan;58(1):272-4
references: 2403534
references: Infect Immun. 1976 Oct;14(4):919-28
references: 11189
references: Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4
references: 388439
references: Indian J Lepr. 1985 Apr-Jun;57(2):278-81
references: 3908575
references: Clin Vaccine Immunol. 2008 Dec;15(12):1755-9
references: 18945881
references: FEBS Lett. 1985 Sep 2;188(2):215-8
references: 2411595
references: J Biol Chem. 1951 Nov;193(1):265-75
references: 14907713
references: J Biol Chem. 1996 May 24;271(21):12333-42
references: 8647835