title: Molecular structural differences between type-2-diabetic and healthy glycogen.
abstract: Glycogen is a highly branched glucose polymer functioning as a glucose buffer in animals. Multiple-detector size exclusion chromatography and fluorophore-assisted carbohydrate electrophoresis were used to examine the structure of undegraded native liver glycogen (both whole and enzymatically debranched) as a function of molecular size, isolated from the livers of healthy and db/db mice (the latter a type 2 diabetic model). Both the fully branched and debranched levels of glycogen structure showed fundamental differences between glycogen from healthy and db/db mice. Healthy glycogen had a greater population of large particles, with more α particles (tightly linked assemblages of smaller β particles) than glycogen from db/db mice. These structural differences suggest a new understanding of type 2 diabetes.
authors: Centre for Nutrition & Food Sciences, The University of Queensland , Brisbane, Qld 4072, Australia.
authors: Mitchell A
authors: MA
authors: Sullivan
authors: Jiong
authors: J
authors: Li
authors: Chuanzhou
authors: C
authors: Li
authors: Francisco
authors: F
authors: Vilaplana
authors: David
authors: D
authors: Stapleton
authors: Angus A
authors: AA
authors: Gray-Weale
authors: Stirling
authors: S
authors: Bowen
authors: Ling
authors: L
authors: Zheng
authors: Robert G
authors: RG
authors: Gilbert
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