title: Development of a mathematical model for evaluating the dynamics of normal and apoptotic Chinese hamster ovary cells.
abstract: A metabolic flux based methodology was developed for modeling the metabolism of a Chinese hamster ovary cell line. The elimination of insignificant fluxes resulted in a simplified metabolic network which was the basis for modeling the significant metabolites. Employing kinetic rate expressions for growing and non-growing subpopulations, a logistic model was developed for cell growth and dynamic models were formulated to describe culture composition and monoclonal antibody (MAb) secretion. The model was validated for a range of nutrient concentrations. Good agreement was obtained between model predictions and experimental data. The ultimate goal of this study is to establish a comprehensive dynamic model which may be used for model-based optimization of the cell culture for MAb production in both batch and fed-batch systems.
authors: Dept. of Chemical Engineering, University of Waterloo, Waterloo, ON, Canada N2L 3G1.
authors: Saeideh
authors: S
authors: Naderi
authors: Mukesh
authors: M
authors: Meshram
authors: Catherine
authors: C
authors: Wei
authors: Brendan
authors: B
authors: McConkey
authors: Brian
authors: B
authors: Ingalls
authors: Hector
authors: H
authors: Budman
authors: Jeno
authors: J
authors: Scharer