title: Quantitative injection from a microloop. Reproducible volumetric sample introduction in capillary zone electrophoresis.
abstract: A wire loop deployed at the tip of a capillary electrophoresis system has been investigated as a means of quantitative injection. A thin film of a liquid is formed on the loop, the loop is transferred to a sealed chamber, and then pneumatic pressure is applied to introduce the contents of the loop into the capillary. As long as the applied pressure is below a certain threshold, no air is introduced into the capillary, even after the loop contents have been fully introduced. Sample surface tension and viscosity do not have a significant effect on the injected volume. The small loop injection technique appears to be a robust and reproducible alternative to presently practiced approaches to sample injection in CE.
authors: Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, Texas 79409-1061.
authors: P K
authors: PK
authors: Dasgupta
authors: K
authors: K
authors: Surowiec