title: Expression of a wheat MYB gene in transgenic tobacco enhances resistance to Ralstonia solanacearum, and to drought and salt stresses.
abstract: MYB transcription factors play diverse roles in plant growth, developmental processes and stress responses. A full-length cDNA sequence of a MYB gene, namely TaPIMP1, was isolated from wheat (Triticum aestivum L.). The TaPIMP1 transcript level was significantly up-regulated by inoculation with a fungal pathogen Bipolaris sorokiniana and by drought treatment. TaPIMP1 encodes the MYB protein TaPIMP1 consisting of 323 amino acids. TaPIMP1 contains two MYB DNA binding domains (R2, R3), two putative nuclear localization sites and two putative transcription activation domains. TaPIMP1 is a new member of the R2R3-MYB transcription factor subfamily. Transient expression in onion epidermal cells of GFP fused with TaPIMP1 proved that subcellular localization of TaPIMP1 occurred in the nucleus. The TaPIMP1 gene was transferred into tobacco (Nicotiana tabacum L.) cultivar W38 by Agrobacterium-mediated transformation. After screening through PCR and RT-PCR analyses, transgenic tobacco lines expressing TaPIMP1 were identified and evaluated for pathogen resistance, and drought and salt tolerance. Compared to untransformed tobacco host plants, TaPIMP1 expressing plants displayed significantly enhanced resistance to Ralstonia solanacearum and exhibited improved tolerances to drought and salt stresses. In these transgenic lines, the activities of phenylalanine ammonia-lyase (PAL) and superoxide dismutase (SOD) were significantly increased relative to wild-type tobacco plants. The results suggested that the wheat R2R3-MYB transcription factor plays an important role in modulating responses to biotic and abiotic stresses.
authors: The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
authors: Hongxia
authors: H
authors: Liu
authors: Xianyao
authors: X
authors: Zhou
authors: Na
authors: N
authors: Dong
authors: Xin
authors: X
authors: Liu
authors: Huaiyu
authors: H
authors: Zhang
authors: Zengyan
authors: Z
authors: Zhang
references: Plant Physiol. 2009 Sep;151(1):275-89
references: 19625633
references: Plant Mol Biol. 2003 Feb;51(3):313-25
references: 12602863
references: Plant Mol Biol. 2004 Aug;55(6):825-34
references: 15604719
references: J Genet Genomics. 2009 Jan;36(1):17-29
references: 19161942
references: Mol Plant Microbe Interact. 2001 Apr;14(4):527-35
references: 11310740
references: Plant Cell Rep. 2010 Aug;29(8):835-44
references: 20490502
references: Plant Cell. 2001 Jan;13(1):113-23
references: 11158533
references: Plant Cell. 2008 Mar;20(3):752-67
references: 18326828
references: Plant Physiol. 2009 May;150(1):244-56
references: 19279197
references: Plant Cell. 2001 Aug;13(8):1891-905
references: 11487700
references: Plant Physiol. 1994 Nov;106(3):877-86
references: 7824656
references: Funct Integr Genomics. 2010 May;10(2):215-26
references: 20225092
references: FEBS Lett. 2006 Jun 12;580(14):3498-504
references: 16730712
references: Biochem Biophys Res Commun. 2007 Feb 9;353(2):299-305
references: 17178106
references: Annu Rev Plant Physiol Plant Mol Biol. 1997 Jun;48:251-275
references: 15012264
references: Plant Mol Biol. 2006 Jan;60(1):107-24
references: 16463103
references: Plant J. 2002 Aug;31(3):279-92
references: 12164808
references: Plant Cell. 2003 Nov;15(11):2551-65
references: 14555693
references: Science. 1985 Mar 8;227(4691):1229-31
references: 17757866
references: Genetics. 1999 Sep;153(1):427-44
references: 10471724
references: Cell. 1998 Jun 26;93(7):1219-29
references: 9657154
references: Trends Plant Sci. 2010 Oct;15(10):573-81
references: 20674465
references: New Phytol. 2010 Apr;186(2):471-83
references: 20149112
references: Plant Physiol. 2003 Feb;131(2):610-20
references: 12586885
references: Methods. 2001 Dec;25(4):402-8
references: 11846609
references: Plant Cell. 2003 Jan;15(1):63-78
references: 12509522
references: J Exp Bot. 2007;58(11):2993-3003
references: 17728298
references: Trends Plant Sci. 2004 Oct;9(10):490-8
references: 15465684
references: Proc Natl Acad Sci U S A. 2002 Jul 23;99(15):10179-84
references: 12119395
references: J Exp Bot. 2010 Jun;61(11):2923-37
references: 20497970
references: Plant Physiol. 2007 Apr;143(4):1739-51
references: 17293435