title: Specific amino acids of Olive mild mosaic virus coat protein are involved in transmission by Olpidium brassicae.
abstract: Transmission of Olive mild mosaic virus (OMMV) is facilitated by Olpidium brassicae (Wor.) Dang. An OMMV mutant (OMMVL11) containing two changes in the coat protein (CP), asparagine to tyrosine at position 189 and alanine to threonine at position 216, has been shown not to be Olpidium brassicae-transmissible owing to inefficient attachment of virions to zoospores. In this study, these amino acid changes were separately introduced into the OMMV genome through site-directed mutagenesis, and the asparagine-to-tyrosine change was shown to be largely responsible for the loss of transmission. Analysis of the structure of OMMV CP by comparative modelling approaches showed that this change is located in the interior of the virus particle and the alanine-to-threonine change is exposed on the surface. The asparagine-to-tyrosine change may indirectly affect attachment via changes in the conformation of viral CP subunits, altering the receptor binding site and thus preventing binding to the fungal zoospore.
authors: Departamento de Fitotecnia, Universidade de Évora, 7002-554 Évora, Portugal.|Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Universidade de Évora, 7002-554 Évora, Portugal.
authors: Carla
authors: C
authors: Varanda
authors: Departamento de Fitotecnia, Universidade de Évora, 7002-554 Évora, Portugal.|Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Universidade de Évora, 7002-554 Évora, Portugal.
authors: Maria do Rosário
authors: MDR
authors: Félix
authors: Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, 2780-157 Oeiras, Portugal.
authors: Cláudio M
authors: CM
authors: Soares
authors: Departamento de Biologia, Universidade de Évora, 7002-554 Évora, Portugal.|Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Universidade de Évora, 7002-554 Évora, Portugal.
authors: Solange
authors: S
authors: Oliveira
authors: Departamento de Fitotecnia, Universidade de Évora, 7002-554 Évora, Portugal.|Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Universidade de Évora, 7002-554 Évora, Portugal.
authors: Maria Ivone
authors: MI
authors: Clara