title: Amplification on chromosomes 1p31, 1q21-24, 5p13-14, and 11p12-14 in ovarian-carcinoma detected by reverse chromosome painting.
abstract: Recently, amplifications of several genes including c-myc, HER-2/neu, and FGF-3 (int-2) have been identified in ovarian carcinomas. We analyzed Il tumor samples from ovarian carcinoma for gene amplification using reverse chromosome painting and standard Southern blot analysis. Reverse chromosome painting detected four amplified domains on chromosome bands 1p31, 1q21-24, 5p13-14, and 11p12-14. None of the amplified domains contained genes previously reported to be amplified in ovarian carcinomas. Southern blot analysis revealed amplifications of genes HER2/neu, N-ras and H-ras. Tumor T3711 showed three independent amplifications including chromosome bands 1p31, 11p12-14 and the HER-2/neu gene (17q11.2-12).
authors: UNIV SAAR,DEPT HUMAN GENET,D-66421 HOMBURG,GERMANY. UNIV SAAR,DEPT PATHOL,D-66421 HOMBURG,GERMANY. UNIV SAAR,DEPT UROL,D-66421 HOMBURG,GERMANY. HENRY FORD HOSP,DEPT SURG,DETROIT,MI 48202.
authors: U
authors: U
authors: Fischer
authors: H
authors: H
authors: Sattler
authors: H
authors: H
authors: Bonkhoff
authors: M
authors: M
authors: Herrmann
authors: B
authors: B
authors: Wullich
authors: E
authors: E
authors: Meese