title: The Tpr protein regulates export of mRNAs with retained introns that traffic through the Nxf1 pathway.
abstract: Post-transcriptional regulation of mRNA includes restriction mechanisms to prevent export and expression of mRNAs that are incompletely spliced. Here we present evidence that the mammalian protein Tpr is involved in this restriction. To study the role of Tpr in export of mRNA with retained introns, we used reporters in which the mRNA was exported either via the Nxf1/Nxt1 pathway using a CTE or via the Crm1 pathway using Rev/RRE. Our data show that even modest knockdown of Tpr using RNAi leads to a significant increase in export and translation from the mRNA containing the CTE. In contrast, Tpr perturbation has no effect on export of mRNA containing the RRE, either in the absence or presence of Rev. Also, no effects were observed on export of a completely spliced mRNA. Taken together, our results indicate that Tpr plays an important role in quality control of mRNA trafficked on the Nxf1 pathway.
authors: Myles H. Thaler Center for AIDS and Human Retrovirus Research and Department of Microbiology, University of Virginia, Charlottesville, Virginia 22908, USA.
authors: John H
authors: JH
authors: Coyle
authors: Yeou-Cherng
authors: YC
authors: Bor
authors: David
authors: D
authors: Rekosh
authors: Marie-Louise
authors: ML
authors: Hammarskjold
references: RNA Biol. 2009 Jul-Aug;6(3):237-41
references: 19574733
references: Genes Dev. 2006 Jun 15;20(12):1597-608
references: 16738405
references: Immunity. 1997 May;6(5):571-82
references: 9175835
references: Mol Biol Cell. 2009 Dec;20(24):5211-23
references: 19864460
references: Science. 2002 Apr 19;296(5567):550-3
references: 11910072
references: Genes Cells. 2002 Apr;7(4):421-34
references: 11952838
references: J Virol. 1998 Sep;72(9):7593-7
references: 9696859
references: Mol Cell Biol. 1988 Sep;8(9):3755-60
references: 3065622
references: J Biol Chem. 2006 Apr 21;281(16):10912-25
references: 16484227
references: J Biol Chem. 1997 Oct 24;272(43):27274-80
references: 9341174
references: Virology. 1973 Apr;52(2):456-67
references: 4705382
references: Cell. 1989 Dec 1;59(5):789-95
references: 2686839
references: Genes Dev. 2003 Dec 15;17(24):3075-86
references: 14701875
references: J Cell Biol. 2006 Nov 6;175(3):415-26
references: 17074885
references: J Mol Biol. 2006 May 12;358(4):997-1009
references: 16580684
references: Gene. 1988 Jun 15;66(1):1-10
references: 3417148
references: J Virol. 1989 Mar;63(3):1265-74
references: 2783738
references: J Virol. 1997 Aug;71(8):5841-8
references: 9223473
references: J Virol. 1994 Feb;68(2):951-8
references: 8289397
references: Nucleic Acids Res. 2010 Aug;38(14):4740-54
references: 20385588
references: Gene. 2009 Jul 1;440(1-2):28-41
references: 19341785
references: EMBO J. 1997 Jun 2;16(11):3256-71
references: 9214641
references: Microbiol Mol Biol Rev. 1999 Jun;63(2):405-45
references: 10357856
references: Exp Cell Res. 2004 May 15;296(1):12-20
references: 15120988
references: Nature. 2002 Dec 12;420(6916):691-5
references: 12478298
references: J Biol Chem. 2008 Oct 3;283(40):27130-43
references: 18682389
references: Methods. 1997 Aug;12(4):288-93
references: 9245608
references: Proc Natl Acad Sci U S A. 1994 Feb 15;91(4):1256-60
references: 8108397
references: Gene. 1986;43(1-2):41-50
references: 3019836
references: RNA. 2004 May;10(5):757-65
references: 15100430
references: Trends Cell Biol. 2010 Aug;20(8):461-9
references: 20627572
references: Mol Cell Biol. 2003 Jan;23(1):92-103
references: 12482964
references: Nature. 2008 Nov 27;456(7221):470-6
references: 18978772
references: Nature. 2006 Sep 14;443(7108):234-7
references: 16971948
references: J Virol. 1994 Dec;68(12):7944-52
references: 7966585
references: J Virol. 1990 Jun;64(6):2743-50
references: 1692347
references: PLoS Genet. 2010 Feb 12;6(2):e1000846
references: 20174442
references: Semin Cell Dev Biol. 1997 Feb;8(1):83-90
references: 15001110
references: Mol Cell Biol. 2001 Apr;21(7):2545-54
references: 11259602
references: J Cell Biol. 2002 Feb 18;156(4):617-30
references: 11839768
references: Bioinformatics. 2003;19 Suppl 1:i118-21
references: 12855447
references: PLoS One. 2007 Sep 05;2(9):e828
references: 17786200
references: Mol Biol Cell. 2004 Sep;15(9):4261-77
references: 15229283
references: J Virol. 1989 May;63(5):1959-66
references: 2704072
references: Cell. 1997 Sep 19;90(6):1051-60
references: 9323133
references: Mol Cell Biol. 2010 Nov;30(21):5168-79
references: 20823268
references: Mol Cell. 1998 Apr;1(5):649-59
references: 9660949
references: J Cell Biol. 1997 Feb 10;136(3):515-29
references: 9024684
references: RNA. 2004 Jul;10(7):1119-29
references: 15208447
references: Mol Cell Biol. 1999 Dec;19(12):8616-24
references: 10567585
references: Cell Cycle. 2010 Feb 1;9(3):580-87
references: 20107313
references: Gene. 2009 Jun 15;439(1-2):71-8
references: 19303045
references: Mol Cell Biol. 2008 Nov;28(22):6954-66
references: 18794356
references: J Virol. 1996 Jun;70(6):3834-43
references: 8648719
references: EMBO J. 2005 Feb 23;24(4):813-23
references: 15692572
references: J Biol Chem. 2007 Jul 6;282(27):19844-53
references: 17513303
references: EMBO J. 1999 May 4;18(9):2593-609
references: 10228171
references: J Biol Chem. 2004 Jul 30;279(31):32897-903
references: 15159391
references: Proc Natl Acad Sci U S A. 2003 Feb 4;100(3):1010-5
references: 12531921
references: Cell. 2004 Jan 9;116(1):63-73
references: 14718167
references: Nature. 1989 Mar 16;338(6212):254-7
references: 2784194
references: J Virol. 1993 Apr;67(4):2266-75
references: 8445731
references: Proc Natl Acad Sci U S A. 1993 Sep 15;90(18):8392-6
references: 7690960