title: OsSGO1 maintains synaptonemal complex stabilization in addition to protecting centromeric cohesion during rice meiosis.
abstract: Shugoshin is a conserved protein in eukaryotes that protects the centromeric cohesin of sister chromatids from cleavage by separase during meiosis. In this study, we identify the rice (Oryza sativa, 2n=2x=24) homolog of ZmSGO1 in maize (Zea mays), named OsSGO1. During both mitosis and meiosis, OsSGO1 is recruited from nucleoli onto centromeres at the onset of prophase. In the Tos17-insertional Ossgo1-1 mutant, centromeres of sister chromatids separate precociously from each other from metaphase I, which causes unequal chromosome segregation during meiosis II. Moreover, the release of OsSGO1 from nucleoli is completely blocked in Ossgo1-1, which leads to the absence of OsSGO1 in centromeric regions after the onset of mitosis and meiosis. Furthermore, the timely assembly and maintenance of synaptonemal complexes during early prophase I are affected in Ossgo1 mutants. Finally, we found that the centromeric localization of OsSGO1 depends on OsAM1, not other meiotic proteins such as OsREC8, PAIR2, OsMER3, or ZEP1.
authors: State Key Laboratory of Plant Genomics and Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors: Mo
authors: M
authors: Wang
authors: Ding
authors: D
authors: Tang
authors: Kejian
authors: K
authors: Wang
authors: Yi
authors: Y
authors: Shen
authors: Baoxiang
authors: B
authors: Qin
authors: Chunbo
authors: C
authors: Miao
authors: Ming
authors: M
authors: Li
authors: Zhukuan
authors: Z
authors: Cheng