title: Quantitative determination of triperpene saponins and alkenated-phenolics from Labisia pumila using an LC-UV/ELSD method and confirmation by LC-ESI-TOF.
abstract: This study describes the first analytical method for the determination of four triterpene saponins (ardisicrenoside B, ardisiacrispin A, 3- O- α- L-rhamnopyranosyl-(1 → 2)-β-D-glucopyranosyl-(1 → 4)-α-L-arabinopynanosyl cyclamiretin A and ardisimamilloside H) and three alkenated-phenolics (irisresorcinol, belamcandol B, and demethylbelamcandaquinone B) from the leaves, leaves/stems, and roots of LABISIA PUMILA using an HPLC-UV-ELSD method. The separation was achieved using a reversed-phase (C-18) column, PDA and ELS detection, and a water/acetonitrile gradient as the mobile phase. The major triterpenoid (ardisiacrispin A) and irisresorcinol compounds were detected at a concentration as low as 10.0 and 0.2 µg/mL, respectively. Analysis of various samples showed considerable variation of 0.11-2.46 % for the major triterpenoid compound, ardisiacrispin A. LC-mass spectrometry method coupled with electrospray ionization (ESI) is described for the identification of compounds in plant samples. This method involved the use of the [M + Na]+ and [M + NH₄]+ ions for compounds 1-4 in the positive ion mode with extractive ion chromatogram (EIC).
authors: National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, University of Mississippi, University, MS, USA.
authors: Bharathi
authors: B
authors: Avula
authors: Yan-Hong
authors: YH
authors: Wang
authors: Zulfiqar
authors: Z
authors: Ali
authors: Troy J
authors: TJ
authors: Smillie
authors: Ikhlas A
authors: IA
authors: Khan