title: Establishment and characterization of a hepatoblastoma cell-line from a patient with secondary acute myeloid-leukemia.
abstract: A girl who had been treated, apparently successfully, with surgery and chemotherapy for a hepatoblastoma, fell ill two years later with what was diagnosed as an AMF M(4). A cell line was established from her peripheral blood. This cell line had epithelial morphology and grew both in suspension culture and as a monolayer. The cells were positive for epithelial surface markers, including the liver-specific alpha-fetoprotein, but not for leukocyte markers. The cell-line's karyotype was markedly abnormal. It did not have any specific aneuploidies or any other aberrations characteristic of leukemias; instead it had gains of 2q and chromosome 20, the most common cytogenetic changes in hepatoblastoma. It is most likely that the patient had a relapse of hepatoblastoma with massive seeding of the blood leading to a leukemia-like picture without, of course, excluding other possibilities.
authors: ODENSE UNIV HOSP,DEPT MED GENET & PATHOL,DK-5000 ODENSE,DENMARK. CHILDRENS HOSP AGLAIA KYRIAKOU,DEPT ONCOL,ATHENS,GREECE.
authors: S
authors: S
authors: Kottaridis
authors: N
authors: N
authors: Pandis
authors: G
authors: G
authors: Bardi
authors: E
authors: E
authors: Panotopoulou
authors: M
authors: M
authors: Baka
authors: H
authors: H
authors: Kosmidis