title: Rotavirus rearranged genomic RNA segments are preferentially packaged into viruses despite not conferring selective growth advantage to viruses.
abstract: The rotavirus (RV) genome consists of 11 double-stranded RNA segments. Sometimes, partial sequence duplication of an RNA segment leads to a rearranged RNA segment. To specify the impact of rearrangement, the replication efficiencies of human RV with rearranged segments 7, 11 or both were compared to these of the homologous human wild-type RV (wt-RV) and of the bovine wt-RV strain RF. As judged by viral growth curves, rotaviruses with a rearranged genome (r-RV) had no selective growth advantage over the homologous wt-RV. In contrast, r-RV were selected over wt-RV during competitive experiments (i.e mixed infections between r-RV and wt-RV followed by serial passages in cell culture). Moreover, when competitive experiments were performed between a human r-RV and the bovine wt-RV strain RF, which had a clear growth advantage, rearranged segments 7, 11 or both always segregated in viral progenies even when performing mixed infections at an MOI ratio of 1 r-RV to 100 wt-RV. Lastly, bovine reassortant viruses that had inherited a rearranged segment 7 from human r-RV were generated. Although substitution of wt by rearranged segment 7 did not result in any growth advantage, the rearranged segment was selected in the viral progenies resulting from mixed infections by bovine reassortant r-RV and wt-RV, even for an MOI ratio of 1 r-RV to 10(7) wt-RV. Lack of selective growth advantage of r-RV over wt-RV in cell culture suggests a mechanism of preferential packaging of the rearranged segments over their standard counterparts in the viral progeny.
authors: Micro-Organismes, Molécules Bioactives et Physiopathologie Intestinale, Université Pierre et Marie Curie-Paris 6, Paris, France.
authors: Cécile
authors: C
authors: Troupin
authors: Aurélie
authors: A
authors: Schnuriger
authors: Sarah
authors: S
authors: Duponchel
authors: Claire
authors: C
authors: Deback
authors: Nathalie
authors: N
authors: Schnepf
authors: Axelle
authors: A
authors: Dehee
authors: Antoine
authors: A
authors: Garbarg-Chenon
references: Virol J. 2006 Jan 03;3:1
references: 16390540
references: J Gen Virol. 1987 Jan;68 ( Pt 1):115-22
references: 3027239
references: Arch Virol. 1993;133(1-2):85-95
references: 8240020
references: J Clin Microbiol. 1987 May;25(5):952-4
references: 3034969
references: Virology. 1996 Oct 15;224(2):446-52
references: 8874505
references: J Gen Virol. 1984 Jul;65 ( Pt 7):1141-50
references: 6086807
references: Arch Virol. 2008;153(3):555-9
references: 18092125
references: J Virol. 2001 Aug;75(16):7305-14
references: 11462002
references: Arch Virol. 1998;143(9):1711-22
references: 9787656
references: J Gen Virol. 1992 Mar;73 ( Pt 3):633-8
references: 1312123
references: Vet Microbiol. 1987 Jan;13(1):27-34
references: 3027953
references: Virology. 2004 Nov 24;329(2):348-60
references: 15518814
references: Virology. 1994 Feb;198(2):567-76
references: 8291239
references: J Virol. 2001 Mar;75(5):2076-86
references: 11160712
references: J Virol. 2008 Apr;82(7):3689-96
references: 18216096
references: J Clin Microbiol. 1999 Apr;37(4):1213-6
references: 10074557
references: J Virol. 2006 Apr;80(8):3801-10
references: 16571797
references: Nucleic Acids Res. 2010 Nov;38(21):7718-35
references: 20671030
references: J Virol. 1993 Nov;67(11):6625-32
references: 8411365
references: J Virol. 1994 Mar;68(3):1682-8
references: 7509001
references: Arch Virol. 1986;89(1-4):161-70
references: 3013127
references: J Virol. 1987 Nov;61(11):3365-72
references: 2822946
references: Virology. 2007 Feb 5;358(1):89-97
references: 16987539
references: J Gen Virol. 1990 Feb;71 ( Pt 2):355-62
references: 2155288
references: Virology. 1985 May;143(1):88-103
references: 2998015
references: Arch Virol. 1994;136(3-4):415-21
references: 8031245
references: J Gen Virol. 2007 Dec;88(Pt 12):3401-3412
references: 18024910
references: J Gen Virol. 1987 Nov;68 ( Pt 11):2961-6
references: 2824672
references: Virus Res. 1987 Jan;6(4):363-82
references: 3564676
references: Acta Virol. 1998 Feb;42(1):55-9
references: 9645244
references: Biochem Biophys Res Commun. 2006 Feb 3;340(1):140-4
references: 16356473
references: Vet Microbiol. 1996 Oct;52(3-4):235-47
references: 8972049
references: Emerg Infect Dis. 2006 Feb;12(2):304-6
references: 16494759
references: Virus Res. 2000 Apr;67(2):163-71
references: 10867195
references: J Virol. 2009 Sep;83(17):8842-8
references: 19553329
references: J Diarrhoeal Dis Res. 1994 Jun;12(2):129-32
references: 7963343
references: Arch Virol. 1992;125(1-4):331-8
references: 1339264
references: J Gen Virol. 1985 Dec;66 ( Pt 12):2703-14
references: 2999313
references: J Virol. 1990 Jan;64(1):120-4
references: 2152809
references: Microbiol Immunol. 1991;35(11):953-61
references: 1663575
references: Adv Virus Res. 1996;46:69-95
references: 8824698
references: J Virol. 2010 Jul;84(13):6711-9
references: 20427539
references: Virology. 1999 Nov 10;264(1):167-80
references: 10544142