title: The Nell-1 growth factor stimulates bone formation by purified human perivascular cells.
abstract: The search for novel sources of stem cells other than bone marrow mesenchymal stem cells (MSCs) for bone regeneration and repair has been a critical endeavor. We previously established an effective protocol to homogeneously purify human pericytes from multiple fetal and adult tissues, including adipose, bone marrow, skeletal muscle, and pancreas, and identified pericytes as a primitive origin of human MSCs. In the present study, we further characterized the osteogenic potential of purified human pericytes combined with a novel osteoinductive growth factor, Nell-1. Purified pericytes grown on either standard culture ware or human cancellous bone chip (hCBC) scaffolds exhibited robust osteogenic differentiation in vitro. Using a nude mouse muscle pouch model, pericytes formed significant new bone in vivo as compared to scaffold alone (hCBC). Moreover, Nell-1 significantly increased pericyte osteogenic differentiation, both in vitro and in vivo. Interestingly, Nell-1 significantly induced pericyte proliferation and was observed to have pro-angiogenic effects, both in vitro and in vivo. These studies suggest that pericytes are a potential new cell source for future efforts in skeletal regenerative medicine, and that Nell-1 is a candidate growth factor able to induce pericyte osteogenic differentiation.
authors: Dental and Craniofacial Research Institute, University of California, Los Angeles, Los Angeles, California 90095-1579, USA.
authors: Xinli
authors: X
authors: Zhang
authors: Bruno
authors: B
authors: Péault
authors: Weiwei
authors: W
authors: Chen
authors: Weiming
authors: W
authors: Li
authors: Mirko
authors: M
authors: Corselli
authors: Aaron W
authors: AW
authors: James
authors: Min
authors: M
authors: Lee
authors: Ronald K
authors: RK
authors: Siu
authors: Pang
authors: P
authors: Shen
authors: Zhong
authors: Z
authors: Zheng
authors: Jia
authors: J
authors: Shen
authors: Jinny
authors: J
authors: Kwak
authors: Janette N
authors: JN
authors: Zara
authors: Feng
authors: F
authors: Chen
authors: Hong
authors: H
authors: Zhang
authors: Zack
authors: Z
authors: Yin
authors: Ben
authors: B
authors: Wu
authors: Kang
authors: K
authors: Ting
authors: Chia
authors: C
authors: Soo
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