title: Pyogranulomatous pleuropneumonia and mediastinitis in ferrets (Mustela putorius furo) associated with Pseudomonas luteola Infection.
abstract: Between 2008 and 2009, three pet ferrets from different sources presented with acute episode of dyspnoea. Cytological examination of pleural exudates revealed severe purulent inflammation with abundant clusters of rod-shaped microorganisms with a clear surrounding halo. Treatment was ineffective and the ferrets died 2-5 days later. Two ferrets were subjected to necropsy examination, which revealed pyothorax, mediastinal lymphadenopathy and multiple white nodules (1-2mm) in the lungs. Microscopical examination showed multifocal necrotizing-pyogranulomatous pleuropneumonia and lymphadenitis with aggregates of encapsulated microorganisms, some of which were positively stained by periodic acid-Schiff and alcian blue. In-situ hybridization for Pneumocystis spp., Ziehl-Neelsen staining and immunohistochemistry for distemper, coronavirus and influenza antigen were negative in all cases. Electron microscopically, the bacteria were 2-3 μm long with a thick electron-lucent capsule. Microbiology from one ferret yielded a pure culture of gram-negative bacteria identified phenotypically as Pseudomonas luteola. This speciation was later confirmed by 16S RNA gene amplification.
authors: Departament de Sanitat i Anatomia Animals, Barcelona, Spain. jorge.martinez.martinez@uab.cat
authors: J
authors: J
authors: Martínez
authors: J
authors: J
authors: Martorell
authors: M L
authors: ML
authors: Abarca
authors: A
authors: A
authors: Olvera
authors: A
authors: A
authors: Ramis
authors: L
authors: L
authors: Woods
authors: N
authors: N
authors: Cheville
authors: C
authors: C
authors: Juan-Sallés
authors: A
authors: A
authors: Moya
authors: A
authors: A
authors: Riera
authors: S
authors: S
authors: Soto
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