title: Nitrifying granules cultivation in a sequencing batch reactor at a low organics-to-total nitrogen ratio in wastewater.
abstract: It is possible to cultivate aerobic granular sludge at a low organic loading rate and organics-to-total nitrogen (COD/N) ratio in wastewater in the reactor with typical geometry (height/diameter = 2.1, superficial air velocity = 6 mm/s). The noted nitrification efficiency was very high (99%). At the highest applied ammonia load (0.3 ± 0.002 mg NH (4) (+) -N g total suspended solids (TSS)(-1) day(-1), COD/N = 1), the dominating oxidized form of nitrogen was nitrite. Despite a constant aeration in the reactor, denitrification occurred in the structure of granules. Applied molecular techniques allowed the changes in the ammonia-oxidizing bacteria (AOB) community in granular sludge to be tracked. The major factor influencing AOB number and species composition was ammonia load. At the ammonia load of 0.3 ± 0.002 mg NH (4) (+) -N g TSS(-1) day(-1), a highly diverse AOB community covering bacteria belonging to both the Nitrosospira and Nitrosomonas genera accounted for ca. 40% of the total bacteria in the biomass.
authors: Department of Environmental Biotechnology, University of Warmia and Mazury in Olsztyn, Słoneczna 45G, 10-709, Olsztyn, Poland. agnieszka.cydzik@uwm.edu.pl
authors: A
authors: A
authors: Cydzik-Kwiatkowska
authors: I
authors: I
authors: Wojnowska-Baryła
references: Water Sci Technol. 2008;58(4):781-7
references: 18776612
references: Appl Microbiol Biotechnol. 2001 Oct;57(1-2):227-33
references: 11693926
references: Appl Environ Microbiol. 1996 Jun;62(6):2156-62
references: 8787412
references: Appl Microbiol Biotechnol. 2007 May;75(1):205-10
references: 17294187
references: Waste Manag. 2006;26(10):1140-7
references: 16289794
references: Biodegradation. 2001;12(5):359-66
references: 11995828
references: Appl Environ Microbiol. 2004 Feb;70(2):1008-16
references: 14766583
references: Biotechnol Adv. 2004 Sep;22(7):533-63
references: 15262316
references: Appl Environ Microbiol. 2000 Nov;66(11):4605-14
references: 11055900
references: J Microbiol Methods. 2002 Jul;50(2):189-203
references: 11997169
references: J Microbiol Methods. 2006 Jul;66(1):1-20
references: 16635533
references: J Water Pollut Control Fed. 1976 May;48(5):835-52
references: 948105
references: Appl Environ Microbiol. 1997 Dec;63(12):4704-12
references: 9406389
references: Bioinformatics. 2009 May 1;25(9):1189-91
references: 19151095
references: Lett Appl Microbiol. 2005;40(5):312-5
references: 15836731
references: Appl Microbiol Biotechnol. 2003 Feb;60(6):687-95
references: 12664147
references: Nucleic Acids Res. 1997 Sep 1;25(17):3389-402
references: 9254694
references: FEMS Microbiol Rev. 2003 Oct;27(4):481-92
references: 14550941
references: Pol J Microbiol. 2012;61(1):41-9
references: 22708345
references: Arch Microbiol. 2002 Feb;177(2):139-49
references: 11807563
references: Chemosphere. 2009 Apr;75(2):220-7
references: 19124145
references: Water Sci Technol. 2004;49(5-6):81-8
references: 15137410
references: Water Sci Technol. 2001;43(3):9-18
references: 11381937