title: First experiences using reflectance confocal microscopy on equivocal skin lesions in Queensland.
abstract: OBJECTIVE
  Reflectance confocal microscopy (RCM) is a non-invasive method of imaging human skin in vivo. The purpose of this study was to observe the experience of using RCM on equivocal skin lesions in a tertiary clinical setting in Queensland.
  
  
  METHODS
  Fifty equivocal lesions on 42 patients were imaged using a reflectance confocal microscope immediately prior to being excised. The images were then analysed blind to the histopathological diagnosis. The experience and problems encountered when using RCM on skin lesions for the first time was also observed.
  
  
  RESULTS
  On RCM analysis 12/13 melanomas (92.3% sensitivity, 75% specificity), 19/22 benign naevi (86% sensitivity, 95% specificity), 6/9 basal cell carcinomas (66.7% sensitivity, 100% specificity)and 6/6 squamous cell carcinomas and its precursors (100% sensitivity, 75% specificity) were diagnosed correctly when using histology as the gold standard. We identified three common problems that affected image quality: object artefacts; positioning artefacts; and movement artefacts.
  
  
  CONCLUSIONS
  Using simple techniques we found that common RCM features were readily identifiable and common artefacts could be minimized, making RCM a useful tool to aid the diagnosis of equivocal skin lesions in a clinical setting.
authors: Dermatology Research Centre, The University of Queensland, School of Medicine, Queensland, Australia. c.curchin@uq.edu.au
authors: Claudia E S
authors: CE
authors: Curchin
authors: Elisabeth M T
authors: EM
authors: Wurm
authors: Duncan Lj
authors: DLj
authors: Lambie
authors: Caterina
authors: C
authors: Longo
authors: Giovanni
authors: G
authors: Pellacani
authors: H Peter
authors: HP
authors: Soyer