title: Improved specificity of a multiplex immunoassay for quantitation of anti-diphtheria toxin antibodies with the use of diphtheria toxoid.
abstract: A nonspecific binding of antibodies to diphtheria toxin, especially in adult serum samples, was observed in our diphtheria-tetanus-pertussis multiplex immunoassay (DTaP4 MIA). This can be significantly reduced by the use of diphtheria toxoid, achieving a good correlation with the Vero cell neutralization test and the toxin binding inhibition assay.
authors: Laboratory for Infectious Diseases and Screening, National Institute of Public Health and the Environment, Bilthoven, Netherlands. Pieter.van.gageldonk@rivm.nl
authors: Pieter G M
authors: PG
authors: van Gageldonk
authors: Christina
authors: C
authors: von Hunolstein
authors: Fiona R M
authors: FR
authors: van der Klis
authors: Guy A M
authors: GA
authors: Berbers
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