title: Serum antibody responses in horses and mice following immunization with Actinobacillus equuli outer membrane proteins and recombinant Aqx toxin.
abstract: The immune responsiveness of mice (without prior natural exposure) and mares (with naturally acquired antibodies) was determined following vaccination with Actinobacillus equuli outer membrane proteins (OMPs) and/or recombinant A. equuli toxin (rAqx). Mice were vaccinated subcutaneously on days 0 and 21 with one of three doses (5, 25 or 50μg) of A. equuli OMPs, rAqx or both, together with Freund's incomplete adjuvant (FIA). Antibodies against formalin-killed whole bacterial cells (WBCs), OMPs and Aqx were determined on days 0, 21 and 42. Mares were vaccinated subcutaneously on days 0 and 21 with 100μg OMPs, 100μg rAqx or a combination of 50μg of each antigen, together with FIA. Antibodies against WBCs, OMPs and Aqx were determined at 7day intervals for the first 42days, as well as on days 56, 70, 154 and 238. Vaccination of mice stimulated an apparent dose response to OMPs and Aqx. Antibodies against OMPs and Aqx were enhanced following vaccination of mares that had naturally acquired pre-existing antibodies. There was no evidence of interference with antibody responses to the individual antigens when OMPs and rAqx were combined prior to vaccination.
authors: Department of Veterinary Pathobiology, 250 McElroy Hall, Oklahoma State University, Center for Veterinary Health Sciences, Stillwater, OK 74078, USA.
authors: Jeffery S
authors: JS
authors: Skaggs
authors: Department of Veterinary Clinical Sciences, 002 Veterinary Medical Teaching Hospital, Center for Veterinary Health Sciences, Stillwater, OK 74078, USA.
authors: G
authors: G
authors: Reed Holyoak
authors: Department of Veterinary Pathobiology, 250 McElroy Hall, Oklahoma State University, Center for Veterinary Health Sciences, Stillwater, OK 74078, USA.
authors: Marie
authors: M
authors: Montelongo
authors: Department of Veterinary Pathobiology, 250 McElroy Hall, Oklahoma State University, Center for Veterinary Health Sciences, Stillwater, OK 74078, USA.
authors: Sahlu
authors: S
authors: Ayalew
authors: Department of Veterinary Pathobiology, 250 McElroy Hall, Oklahoma State University, Center for Veterinary Health Sciences, Stillwater, OK 74078, USA. Electronic address: anthony.confer@okstate.edu.
authors: Anthony W
authors: AW
authors: Confer