title: [Hydrolysis technology optimization of phorbol esters by orthogonal experiment].
abstract: OBJECTIVE
  To establish the best hydrolytic conditions from phorbol esters.
  
  
  METHODS
  The orthogonal experiment was used to optimize 4 factors, which were reaction time, ratio of solid-to-liquid, hydrolytic times, and temperature. Diamonsil C18 column (4. 6 mm x 250 mm, 5 microm) was used and the mobile phase was consisted of acetonitrile and water for HPLC detection. The detection wavelength was set at 234 nm, the flow rate was 1 mL x min(-1), and the column temperature was 25 degrees C.
  
  
  RESULTS
  The optimum conditions were 10 h of reaction time, 1:6 of solid-to-liquid (BaOH/MeOH) ratio, 25 degrees C of temperature, and one time of hydrolysis. There was a good linear relationship of phorbol in the range of 4.28-107 mg x L(-1) (r = 0.999 9), and the average recovery was 97.89%, with RSD 0.78%.
  
  
  CONCLUSIONS
  The method is steady, reliable and reproducible, and it provides a mean for future study.
authors: College of Pharmacy, Chongqing Medical University, Chongqing 400016, China. guohu8606@yahoo.cn
authors: Hu
authors: H
authors: Guo
authors: Fei
authors: F
authors: Peng
authors: Lianju
authors: L
authors: Ma
authors: Zhan
authors: Z
authors: Jiang
authors: Xin
authors: X
authors: Liu