title: Antagonistic regulation of EMT by TIF1γ and Smad4 in mammary epithelial cells.
abstract: TGF-β is a potent inducer of epithelial-to-mesenchymal transition (EMT), a process involved in tumour invasion. TIF1γ participates in TGF-β signalling. To understand the role of TIF1γ in TGF-β signalling and its requirement for EMT, we analysed the TGF-β1 response of human mammary epithelial cell lines. A strong EMT increase was observed in TIF1γ-silenced cells after TGF-β1 treatment, whereas Smad4 inactivation completely blocked this process. Accordingly, the functions of several TIF1γ target genes can be linked to EMT, as shown by microarray analysis. As a negative regulator of Smad4, TIF1γ could be crucial for the regulation of TGF-β signalling. Furthermore, TIF1γ binds to and represses the plasminogen activator inhibitor 1 promoter, demonstrating a direct role of TIF1γ in TGF-β-dependent gene expression. This study shows the molecular relationship between TIF1γ and Smad4 in TGF-β signalling and EMT.
authors: Inserm U1052, Centre de Recherche en Cancérologie de Lyon, F-69000 Lyon, France; CNRS UMR5286, F-69000 Lyon, France; Centre Léon Bérard, F-69000 Lyon, France.
authors: Cédric
authors: C
authors: Hesling
authors: Laurent
authors: L
authors: Fattet
authors: Guillaume
authors: G
authors: Teyre
authors: Delphine
authors: D
authors: Jury
authors: Philippe
authors: P
authors: Gonzalo
authors: Jonathan
authors: J
authors: Lopez
authors: Christophe
authors: C
authors: Vanbelle
authors: Anne-Pierre
authors: AP
authors: Morel
authors: Germain
authors: G
authors: Gillet
authors: Ivan
authors: I
authors: Mikaelian
authors: Ruth
authors: R
authors: Rimokh
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