title: Isotope-coded chemical reporter and acid-cleavable affinity reagents for monitoring protein sulfenic acids.
abstract: We have developed an approach that allows relative quantification of protein sulfenic acids using a pair of light and heavy isotope labled probes, DAz-2 and d(6)-DAz-2. In conjunction with a new complementary acid-cleavable linker, Yn-ACL, we demonstrate that tagged peptides are successfully labeled, enriched, and fully characterized by LC-MS/MS analysis. Overall, this method can be applied to map sites of cysteine oxidation and compare protein sulfenylation in normal and disease states.
authors: Department of Chemistry, University of Michigan, Ann Arbor, MI 48109, USA.
authors: Thu H
authors: TH
authors: Truong
authors: Francisco J
authors: FJ
authors: Garcia
authors: Young Ho
authors: YH
authors: Seo
authors: Kate S
authors: KS
authors: Carroll