title: Genetic and expression analysis of cattle identifies candidate genes in pathways responding to Trypanosoma congolense infection.
abstract: African bovine trypanosomiasis caused by Trypanosoma sp., is a major constraint on cattle productivity in sub-Saharan Africa. Some African Bos taurus breeds are highly tolerant of infection, but the potentially more productive Bos indicus zebu breeds are much more susceptible. Zebu cattle are well adapted for plowing and haulage, and increasing their tolerance of trypanosomiasis could have a major impact on crop cultivation as well as dairy and beef production. We used three strategies to obtain short lists of candidate genes within QTL that were previously shown to regulate response to infection. We analyzed the transcriptomes of trypanotolerant N'Dama and susceptible Boran cattle after infection with Trypanosoma congolense. We sequenced EST libraries from these two breeds to identify polymorphisms that might underlie previously identified quantitative trait loci (QTL), and we assessed QTL regions and candidate loci for evidence of selective sweeps. The scan of the EST sequences identified a previously undescribed polymorphism in ARHGAP15 in the Bta2 trypanotolerance QTL. The polymorphism affects gene function in vitro and could contribute to the observed differences in expression of the MAPK pathway in vivo. The expression data showed that TLR and MAPK pathways responded to infection, and the former contained TICAM1, which is within a QTL on Bta7. Genetic analyses showed that selective sweeps had occurred at TICAM1 and ARHGAP15 loci in African taurine cattle, making them strong candidates for the genes underlying the QTL. Candidate QTL genes were identified in other QTL by their expression profile and the pathways in which they participate.
authors: School of Biological Sciences, University of Liverpool, Liverpool L69 7ZB, United Kingdom.
authors: Harry
authors: H
authors: Noyes
authors: Andy
authors: A
authors: Brass
authors: Isaiah
authors: I
authors: Obara
authors: Susan
authors: S
authors: Anderson
authors: Alan L
authors: AL
authors: Archibald
authors: Dan G
authors: DG
authors: Bradley
authors: Paul
authors: P
authors: Fisher
authors: Abigail
authors: A
authors: Freeman
authors: John
authors: J
authors: Gibson
authors: Michael
authors: M
authors: Gicheru
authors: Laurence
authors: L
authors: Hall
authors: Olivier
authors: O
authors: Hanotte
authors: Helen
authors: H
authors: Hulme
authors: Declan
authors: D
authors: McKeever
authors: Caitriona
authors: C
authors: Murray
authors: Sung Jung
authors: SJ
authors: Oh
authors: Catriona
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authors: Tate
authors: Ken
authors: K
authors: Smith
authors: Miika
authors: M
authors: Tapio
authors: John
authors: J
authors: Wambugu
authors: Diana J
authors: DJ
authors: Williams
authors: Morris
authors: M
authors: Agaba
authors: Stephen J
authors: SJ
authors: Kemp
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