title: Detection of the circulating antigen 14-3-3 protein of Schistosoma japonicum by time-resolved fluoroimmunoassay in rabbits.
abstract: BACKGROUND
  Schistosomiasis remains a major public health concern that afflicts millions of people worldwide. Low levels of Schistosoma infection require more sensitive diagnostic methods. In this study, a time-resolved fluoroimmunoassay (TRFIA) was developed for detecting the signal transduction protein 14-3-3, a circulating antigen of Schistosoma japonicum.
  
  
  RESULTS
  The detection limit of 14-3-3-TRFIA was 0.78 ng/ml, with a linear measurement range from 0.78 to 800 ng/ml. The average intra-assay and inter-assay variability of this TRFIA was 8.9% and 12.2% respectively, and the mean recovery rate ranged from 92.1% to 115.5%. Within the first 21 days post-infection in rabbits, the positive rates of the 14-3-3-TRFIA were distinctly higher compared to ELISA. All these findings illustrate that 14-3-3-TRFIA has a higher detection efficacy and is a good early diagnostic method for active Schistosoma infection.
  
  
  CONCLUSIONS
  A sandwich TRFIA for detecting the circulating antigen 14-3-3 of S. japonicum has been developed, and has demonstrated to be a good potential diagnostic method for schistosomiasis.
authors: Jiangsu Institute of Parasitic Diseases, Wuxi, Jiangsu Province, China.
authors: Chun-Yan
authors: CY
authors: Qian
authors: Biao
authors: B
authors: Huang
authors: Chuan-Xin
authors: CX
authors: Yu
authors: Jue
authors: J
authors: Zhang
authors: Xu-Ren
authors: XR
authors: Yin
authors: Jie
authors: J
authors: Wang
authors: Li-Jun
authors: LJ
authors: Song
authors: Wei
authors: W
authors: Zhang
authors: Xue-Dan
authors: XD
authors: Ke
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