title: Cloning and expression of a bpr gene encoding Bacillopeptidase F from Bacillus amyloliquefaciens CH86-1.
abstract: A gene encoding bacillopeptidase F, bpr86-1, was cloned from B. amyloliquefaciens CH86-1 isolated from cheonggukjang. This gene could encode a preproenzyme of 1,431 amino acids. When bpr86-1 was introduced into B. subtilis WB600 via pHY300PLK, an E. coli-Bacillus shuttle vector, the transformant showed fibrinolytic activity. During growth on LB, the fibrinolytic activity of cells increased sharply when they entered the stationary phase. The highest activity (761.4 mU/mg protein) was observed at 96 h of cultivation.
authors: Division of Applied Life Science (BK21 Program), Graduate School, Gyeongsang National University, Jinju, Korea.
authors: Gun-Hee
authors: GH
authors: Kwon
authors: Jae-Yong
authors: JY
authors: Park
authors: Jong-Sang
authors: JS
authors: Kim
authors: Jinkyu
authors: J
authors: Lim
authors: Cheon-Seok
authors: CS
authors: Park
authors: Dae Young
authors: DY
authors: Kwon
authors: Jeong Hwan
authors: JH
authors: Kim