title: Prevalence of Cryptosporidium spp. in pigs in Shanghai, China.
abstract: A survey on prevalence of Cryptosporidium spp. in pigs at 12 farms in 8 suburban districts and 1 county of Shanghai was conducted under Sheather's sucrose flotation protocol and modified acid-fast stain methods from 2006 to 2009. A total of 2323 faecal samples were collected and Cryptosporidium spp. oocysts were detected in 800 samples (34.4%). Cryptosporidium was found in all 12 pig farms. Significant variations of prevalence were observed in different farms ranging from 14.1% to 90.6%. A follow-up survey on a positive pig farm for 13 consecutive months revealed that the most serious infection of Cryptosporidium spp. in pigs happened in winter and spring, and the lowest infection season was summer. Cryptosporidium spp. infection was mainly found in piglets within 2 months and no infection was found among those pigs of 90-180 days of age. The genotype analyses were carried out through PCR-RFLP and partial sequences analysis of small subunit ribosomal RNA (SSU rRNA) in some of the positive samples. Cryptosporidium suis (57/69, 82.6%), Cryptosporidium pig genotype II (6/69, 8.7%) and mixed infection of above two species/genotype (6/69, 8.7%) were found to be the main species/genotype in pigs in Shanghai area.
authors: Key Laboratory of Animal Parasitology of Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 518, Ziyue Road, Minhang District, Shanghai 200241, People's Republic of China. zhaoguochen@shvri.ac.cn
authors: Zhaoguo
authors: Z
authors: Chen
authors: Rongsheng
authors: R
authors: Mi
authors: Huizhu
authors: H
authors: Yu
authors: Yaojun
authors: Y
authors: Shi
authors: Yan
authors: Y
authors: Huang
authors: Yongjun
authors: Y
authors: Chen
authors: Peng
authors: P
authors: Zhou
authors: Youmin
authors: Y
authors: Cai
authors: Jiaojiao
authors: J
authors: Lin