title: [Primary culture and adipogenic process of pre-adipocytes from infrapatellar fat pad of osteoarthritic patients].
abstract: OBJECTIVE
  To investigate the primary culture and adipogenic process of pre-adipocytes from infrapatellar fat pad of osteoarthritic patients.
  
  
  METHODS
  The pre-adipocytes were isolated by enzymatic digestion. The morphological changes of cultured cells were observed and the growth curve was drawn by CCK-8 method. During the adipogenic process, the intracytoplasmic lipid of differentiated cells was determined by oil red O staining. And the adiponectin levels in the culture supernatants were measured by ELISA (enzyme-linked immunosorbent assay).
  
  
  RESULTS
  The primary cultured fibroblast-like cells were spindle-shaped. In the process of adipogenesis, the intracytoplasmic lipid droplets were observed at Day 3 and over 80% of the cells differentiated into adipocytes at Day 21. With the increasing number of adipocytes, the adiponectin levels in the culture supernatant elevated and peaked at Week 3. The differentiated cells were proven to be adipocytes functioning actively.
  
  
  CONCLUSIONS
  The primary culture and adipogenic process of pre-adipocytes in infrapatellar fat pad of osteoarthritic patients has been successfully established. Thus it may provide an ideal model for the study of endocrine function of infrapatellar fat pad and understanding its role in the pathogenesis of osteoarthritis.
authors: Department of Orthopedics, Chinese Academy of Medical Sciences, Beijing 100730, China.
authors: Yuan-hui
authors: YH
authors: Duan
authors: Ming
authors: M
authors: Li
authors: Dong-sheng
authors: DS
authors: Hao
authors: De-ling
authors: DL
authors: Li
authors: Zhi-hong
authors: ZH
authors: Wu
authors: Gui-xing
authors: GX
authors: Qiu