title: Transcription factor Nkx2-3 controls the vascular identity and lymphocyte homing in the spleen.
abstract: The vasculature in the spleen and peripheral lymph nodes (pLNs) is considerably different, which affects both homing of lymphocytes and antigenic access to these peripheral lymphoid organs. In this paper, we demonstrate that in mice lacking the homeodomain transcription factor Nkx2-3, the spleen develops a pLN-like mRNA expression signature, coupled with the appearance of high endothelial venules (HEVs) that mediate L-selectin-dependent homing of lymphocytes into the mutant spleen. These ectopic HEV-like vessels undergo postnatal maturation and progressively replace MAdCAM-1 by pLN addressin together with the display of CCL21 arrest chemokine in a process that is reminiscent of HEV formation in pLNs. Similarly to pLNs, development of HEV-like vessels in the Nkx2-3-deficient spleen depends on lymphotoxin-β receptor-mediated signaling. The replacement of splenic vessels with a pLN-patterned vasculature impairs the recirculation of adoptively transferred lymphocytes and reduces the uptake of blood-borne pathogens. The Nkx2-3 mutation in BALB/c background causes a particularly disturbed splenic architecture, characterized by the near complete lack of the red pulp, without affecting lymph nodes. Thus, our observations reveal that the organ-specific patterning of splenic vasculature is critically regulated by Nkx2-3, thereby profoundly affecting the lymphocyte homing mechanism and blood filtering capacity of the spleen in a tissue-specific manner.
authors: Department of Immunology and Biotechnology, Faculty of Medicine, University of Pécs, H-7624 Pécs, Hungary.
authors: Tamás
authors: T
authors: Czömpöly
authors: Arpád
authors: A
authors: Lábadi
authors: Zoltán
authors: Z
authors: Kellermayer
authors: Katalin
authors: K
authors: Olasz
authors: Hans-Henning
authors: HH
authors: Arnold
authors: Péter
authors: P
authors: Balogh