title: Transcriptomic and proteomic profiling of maize embryos exposed to camptothecin.
abstract: BACKGROUND
  Camptothecin is a plant alkaloid that specifically binds topoisomerase I, inhibiting its activity and inducing double stranded breaks in DNA, activating the cell responses to DNA damage and, in response to severe treatments, triggering cell death.
  
  
  RESULTS
  Comparative transcriptomic and proteomic analyses of maize embryos that had been exposed to camptothecin were conducted. Under the conditions used in this study, camptothecin did not induce extensive degradation in the genomic DNA but induced the transcription of genes involved in DNA repair and repressed genes involved in cell division. Camptothecin also affected the accumulation of several proteins involved in the stress response and induced the activity of certain calcium-dependent nucleases. We also detected changes in the expression and accumulation of different genes and proteins involved in post-translational regulatory processes.
  
  
  CONCLUSIONS
  This study identified several genes and proteins that participate in DNA damage responses in plants. Some of them may be involved in general responses to stress, but others are candidate genes for specific involvement in DNA repair. Our results open a number of new avenues for researching and improving plant resistance to DNA injury.
authors: Department of Molecular Genetics, Centre for Research in Agricultural Genomics, Campus UAB, Edifici CRAG, Bellaterra (Cerdanyola del Vallés), 08193 Barcelona, Spain.
authors: Nuria
authors: N
authors: Sánchez-Pons
authors: Sami
authors: S
authors: Irar
authors: Nora
authors: N
authors: García-Muniz
authors: Carlos M
authors: CM
authors: Vicient
references: Cancer Res. 2002 Mar 15;62(6):1688-95
references: 11912141
references: Plant J. 1999 Oct;20(1):109-17
references: 10571870
references: Electrophoresis. 1999 Nov;20(17):3472-8
references: 10608717
references: Plant Mol Biol. 2006 Apr;60(6):929-46
references: 16724262
references: Planta. 2006 Mar;223(4):755-68
references: 16231157
references: Nat Rev Cancer. 2006 Oct;6(10):789-802
references: 16990856
references: Brief Funct Genomic Proteomic. 2004 Nov;3(3):212-9
references: 15642185
references: BMC Syst Biol. 2011 Feb 25;5:33
references: 21352515
references: Nat Rev Mol Cell Biol. 2009 Jun;10(6):430-6
references: 19461665
references: Int Rev Cell Mol Biol. 2008;270:87-144
references: 19081535
references: Nat Rev Mol Cell Biol. 2008 Aug;9(8):616-27
references: 18594563
references: Science. 2008 Dec 5;322(5907):1511-6
references: 19023046
references: Science. 2007 May 25;316(5828):1160-6
references: 17525332
references: Mol Cell Proteomics. 2002 Apr;1(4):304-13
references: 12096112
references: Ann Bot. 2002 Aug;90(2):287-92
references: 12197527
references: Trends Plant Sci. 2009 Apr;14(4):229-36
references: 19285908
references: Plant J. 2003 Oct;36(1):94-104
references: 12974814
references: J Proteomics. 2010 Aug 5;73(9):1707-21
references: 20566372
references: Plant Mol Biol. 2000 Oct;44(3):387-97
references: 11199396
references: Trends Plant Sci. 2004 Apr;9(4):174-9
references: 15063867
references: Proteomics. 2002 Sep;2(9):1131-45
references: 12362332
references: Curr Biol. 2001 Jun 5;11(11):R423-6
references: 11516663
references: Plant Physiol. 2008 Sep;148(1):479-89
references: 18633122
references: Annu Rev Plant Biol. 2004;55:555-90
references: 15377232
references: Nucleic Acids Res. 1996 Nov 1;24(21):4242-8
references: 8932379
references: Plant Cell Environ. 2006 May;29(5):754-63
references: 17087460
references: J Cell Biol. 2000 Jun 26;149(7):1405-18
references: 10871281
references: Plant J. 2008 Aug;55(3):526-42
references: 18419781
references: Nature. 2000 Oct 12;407(6805):777-83
references: 11048728
references: Cell Mol Life Sci. 2004 Feb;61(3):336-54
references: 14770297
references: Plant Physiol Biochem. 2008 Dec;46(12):1062-70
references: 18755596
references: J Photochem Photobiol B. 2006 Aug 1;84(2):150-60
references: 16624568
references: Genome Biol. 2004;5(3):R16
references: 15003119
references: FEBS Lett. 2001 Feb 2;489(2-3):237-42
references: 11165257
references: Planta. 2000 Oct;211(5):656-62
references: 11089678
references: Curr Opin Cell Biol. 2005 Dec;17(6):590-5
references: 16229998
references: Mol Cell Biol. 1999 Mar;19(3):1720-30
references: 10022859
references: Planta. 1989 Sep;179(2):181-95
references: 24201517
references: Plant J. 2004 Apr;38(2):366-79
references: 15078338
references: Mutat Res. 2005 Apr 1;571(1-2):235-47
references: 15748650
references: Trends Plant Sci. 2005 Feb;10(2):79-87
references: 15708345
references: Annu Rev Biochem. 2001;70:369-413
references: 11395412
references: Mol Cell Proteomics. 2006 Mar;5(3):484-96
references: 16316980
references: J Biol Chem. 2008 Jul 25;283(30):21074-83
references: 18515798
references: EMBO J. 2009 Jul 22;28(14):2090-9
references: 19536137
references: Plant Physiol. 2004 Oct;136(2):3080-94; discussion 3001-2
references: 15466235
references: Plant Physiol. 2006 Aug;141(4):1349-62
references: 16798950
references: FEMS Microbiol Lett. 2008 Aug;285(1):10-5
references: 18510556
references: Plant J. 2006 Dec;48(6):947-61
references: 17227549
references: Biochem Soc Trans. 2004 Dec;32(Pt 6):964-6
references: 15506937
references: Annu Rev Plant Physiol Plant Mol Biol. 1996 Jun;47:75-100
references: 15012283
references: Int Rev Cytol. 2006;255:1-40
references: 17178464
references: Trends Genet. 2003 Jun;19(6):321-9
references: 12801725
references: BMC Genomics. 2009 Oct 12;10:467
references: 19821986
references: Curr Opin Struct Biol. 2008 Jun;18(3):290-8
references: 18515081
references: Antimicrob Agents Chemother. 2011 Jan;55(1):146-54
references: 20974863
references: Nat Cell Biol. 2004 Sep;6(9):820-30
references: 15311282
references: Proc Natl Acad Sci U S A. 1993 Jul 15;90(14):6656-60
references: 8393572
references: Nucleic Acids Res. 2006;34(15):4126-37
references: 16935876
references: Plant Cell. 2007 Oct;19(10):3080-9
references: 17921313
references: Cancer Res. 2001 Aug 1;61(15):5926-32
references: 11479235
references: Plant Cell. 2001 Nov;13(11):2471-81
references: 11701882
references: Genetics. 2007 Jul;176(3):1469-82
references: 17507687
references: Cell. 2003 Sep 5;114(5):623-34
references: 13678585
references: J Exp Bot. 2001 Jun;52(359):1367-73
references: 11432956
references: J Exp Bot. 2007;58(13):3591-607
references: 17916636
references: Plant Cell. 2008 Oct;20(10):2783-97
references: 18941054
references: Cancer Res. 1992 Apr 1;52(7):1823-9
references: 1312900
references: Plant J. 2003 Sep;35(6):771-86
references: 12969430
references: J Exp Bot. 2008;59(4):939-50
references: 18304977
references: Plant J. 2003 Oct;36(1):1-11
references: 12974806
references: Plant J. 1995 Dec;8(6):865-76
references: 8580959
references: Planta. 1996;199(1):1-8
references: 8680303
references: Plant J. 2009 Jun;58(5):791-802
references: 19187040
references: Plant Cell. 2002 Sep;14(9):2085-93
references: 12215507
references: Plant Mol Biol. 1991 Jan;16(1):59-70
references: 1653630
references: J Cell Sci. 2002 Aug 15;115(Pt 16):3227-34
references: 12140254
references: Eukaryot Cell. 2006 Oct;5(10):1688-704
references: 17030995
references: Plant Physiol. 2009 Sep;151(1):461-71
references: 19571309
references: Plant J. 2010 Jun 1;62(5):727-41
references: 20230486
references: PLoS One. 2007 May 09;2(5):e430
references: 17487278
references: Plant Physiol Biochem. 2005 Sep;43(9):828-35
references: 16289945
references: Gene. 2006 Feb 15;367:17-37
references: 16337753
references: Carcinogenesis. 2002 May;23(5):687-96
references: 12016139
references: Electrophoresis. 2002 Jun;23(11):1591-8
references: 12179976
references: J Biol Chem. 2006 Mar 3;281(9):5575-81
references: 16407260
references: Med Oncol. 2011 Dec;28(4):1302-7
references: 20652449
references: PLoS Genet. 2007 Oct;3(10):1894-906
references: 17937504
references: Mol Cell Proteomics. 2004 Oct;3(10):960-9
references: 15238602
references: Plant Physiol. 2009 Nov;151(3):1421-32
references: 19759339
references: Genetics. 2005 Jun;170(2):591-600
references: 15834151
references: Proteomics. 2004 Feb;4(2):383-96
references: 14760708
references: Nucleic Acids Res. 2003 Sep 1;31(17):5016-24
references: 12930951
references: Plant Physiol. 1998 Aug;117(4):1253-63
references: 9701581
references: Mutat Res. 1996 Oct 18;364(2):57-71
references: 8879272