title: A rapid and inexpensive method for the direct PCR amplification of DNA from plants.
abstract: OBJECTIVE
  We present a rapid and inexpensive alternative to DNA isolation for polymerase chain reaction (PCR) amplification from plants. •
  
  
  RESULTS
  The method involves direct PCR amplification from material macerated in one buffer, followed by dilution and incubation in a second buffer. We describe the procedure and demonstrate its application for nuclear and plastid DNA amplification across a broad range of vascular plants. •
  
  
  CONCLUSIONS
  The method is fast, easy to perform, cost-effective, and consequently ideal for large sample numbers. It represents a considerable simplification of present approaches requiring DNA isolation prior to PCR amplification and will be useful in plant systematics and biotechnology, including applications such as DNA barcoding.
authors: Department of Biochemistry, University of Stellenbosch, Stellenbosch, South Africa.
authors: Dirk U
authors: DU
authors: Bellstedt
authors: Michael D
authors: MD
authors: Pirie
authors: J Christiaan
authors: JC
authors: Visser
authors: Margaret J
authors: MJ
authors: de Villiers
authors: Berit
authors: B
authors: Gehrke