title: Placental protein 13 (PP13/galectin-13) undergoes lipid raft-associated subcellular redistribution in the syncytiotrophoblast in preterm preeclampsia and HELLP syndrome.
abstract: OBJECTIVE
  To investigate placental protein 13 (PP13) localization in relation to cytoskeleton and lipid rafts in preeclampsia and HELLP syndrome.
  
  
  METHODS
  Placental cryosections from patients with preeclampsia and HELLP, and controls were stained for PP13, actin, PLAP (lipid raft marker), and CD71 (nonraft marker). BeWo cells exposed to stress conditions were stained for PP13 and actin. Protein localizations were investigated by confocal microscopy, PP13 concentrations by ELISA.
  
  
  RESULTS
  PP13-actin colocalization was increased in syncytiotrophoblast juxtamembrane regions in term/preterm preeclampsia and HELLP. PP13-CD71 colocalization was decreased and PP13-PLAP proximity was increased in preterm but not term preeclampsia and HELLP. PP13-release from BeWo cells was inhibited by cytoskeleton disruption, and augmented by Ca2+-influx and ischemic stress.
  
  
  CONCLUSIONS
  The actin cytoskeleton, probably in connection with lipid rafts, controls trophoblastic "nonclassical" PP13 export. PP13 is released from the syncytiotrophoblast in preterm preeclampsia and HELLP, mimicked in BeWo cells by ischemic stress, suggesting PP13 is a placental alarmin.
authors: Immunology Research Group of the Hungarian Academy of Sciences, Budapest, Hungary.
authors: Andrea
authors: A
authors: Balogh
authors: Judit
authors: J
authors: Pozsgay
authors: János
authors: J
authors: Matkó
authors: Zhong
authors: Z
authors: Dong
authors: Chong Jai
authors: CJ
authors: Kim
authors: Tibor
authors: T
authors: Várkonyi
authors: Marei
authors: M
authors: Sammar
authors: János
authors: J
authors: Rigó
authors: Hamutal
authors: H
authors: Meiri
authors: Roberto
authors: R
authors: Romero
authors: Zoltán
authors: Z
authors: Papp
authors: Nándor Gábor
authors: NG
authors: Than
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