title: Comparative study of hematopoietic differentiation between human embryonic stem cell lines.
abstract: Directed differentiation of human embryonic stem cells (hESCs) into any desired cell type has been hailed as a therapeutic promise to cure many human diseases. However, substantial roadblocks still exist for in vitro differentiation of hESCs into distinct cell types, including T lymphocytes. Here we examined the hematopoietic differentiation potential of six different hESC lines. We compare their ability to develop into CD34(+) or CD34(+)CD45(+) hematopoietic precursor populations under several differentiation conditions. Comparison of lymphoid potential of hESC derived- and fetal tissue derived-hematopoietic precursors was also made. We found diverse hematopoietic potential between hESC lines depending on the culture or passage conditions. In contrast to fetal-derived hematopoietic precursors, none of the CD34(+) precursors differentiated from hESCs were able to develop further into T cells. These data underscore the difficulties in the current strategy of hESC forward differentiation and highlight distinct differences between CD34(+) hematopoietic precursors generated in vitro versus in vivo.
authors: Division of Immunology and Pathogenesis, Department of Molecular and Cell Biology, University of California, Berkeley, California, United States of America.
authors: Heather
authors: H
authors: Melichar
authors: Ou
authors: O
authors: Li
authors: Jenny
authors: J
authors: Ross
authors: Hilary
authors: H
authors: Haber
authors: Dragana
authors: D
authors: Cado
authors: Hector
authors: H
authors: Nolla
authors: Ellen A
authors: EA
authors: Robey
authors: Astar
authors: A
authors: Winoto
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