title: Local gene delivery system by bubble liposomes and ultrasound exposure into joint synovium.
abstract: Recently, we have developed novel polyethylene glycol modified liposomes (bubble liposomes; BL) entrapping an ultrasound (US) imaging gas, which can work as a gene delivery tool with US exposure. In this study, we investigated the usefulness of US-mediated gene transfer systems with BL into synoviocytes in vitro and joint synovium in vivo. Highly efficient gene transfer could be achieved in the cultured primary synoviocytes transfected with the combination of BL and US exposure, compared to treatment with plasmid DNA (pDNA) alone, pDNA plus BL, or pDNA plus US. When BL was injected into the knee joints of mice, and US exposure was applied transcutaneously to the injection site, highly efficient gene expression could be observed in the knee joint transfected with the combination of BL and US exposure, compared to treatment with pDNA alone, pDNA plus BL, or pDNA plus US. The localized and prolonged gene expression was also shown by an in vivo luciferase imaging system. Thus, this local gene delivery system into joint synovium using the combination of BL and US exposure may be an effective means for gene therapy in joint disorders.
authors: Department of Drug and Gene Delivery Systems, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.
authors: Yoichi
authors: Y
authors: Negishi
authors: Yuka
authors: Y
authors: Tsunoda
authors: Yoko
authors: Y
authors: Endo-Takahashi
authors: Yusuke
authors: Y
authors: Oda
authors: Ryo
authors: R
authors: Suzuki
authors: Kazuo
authors: K
authors: Maruyama
authors: Matsuo
authors: M
authors: Yamamoto
authors: Yukihiko
authors: Y
authors: Aramaki
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