title: Purification and structural characterization of fengycin homologues produced by Bacillus subtilis LSFM-05 grown on raw glycerol.
abstract: Raw glycerol is a byproduct of biodiesel production that currently has low to negative value for biodiesel producers. One option for increasing the value of raw glycerol is to use it as a feedstock for microbial production. Bacillus subtilis LSFM 05 was used for the production of fengycin in a mineral medium containing raw glycerol as the sole carbon source. Fengycin was isolated by acid precipitation at pH 2 and purified by silica gel column chromatography and characterized using electrospray ionization (ESI) Fourier transform ion cyclotron resonance mass spectrometry (ESI FT-ICR MS) with collision-induced dissociation (CID). The mass spectrum revealed the presence of the ions of m/z 1,435.7, 1,449.9, 1,463.8, 1,477.8, 1,491.8 and 1,505.8, which were further fragmented by ESI-MS/MS. The CID profile showed the presence of a series of ions (m/z 1,080 and 966) and (m/z 1,108 and 994) that represented the different fengycin homologues A and B, respectively. Fengycin homologues A and B are variants that differ at position 6 of the peptide moiety, having either Ala or Val residues, respectively. Mass spectrometry analyses identified four fengycin A and three fengycin B variants with fatty acid components containing 14-17 carbons. These results demonstrate that raw glycerol can be used as feedstock to produce fengycin, and additional work should focus on the optimization of process conditions to increase productivity.
authors: Department of Food Science, Food Engineering Faculty, University of Campinas, (UNICAMP), Rua Monteiro Lobato 80, Cidade Universitária Zeferino Vaz, Campinas, SP, 13083-862, Brazil. an.ffaria@gmail.com
authors: Andreia Fonseca
authors: AF
authors: de Faria
authors: Diego
authors: D
authors: Stéfani
authors: Boniek Gontijo
authors: BG
authors: Vaz
authors: Ísis Serrano
authors: ÍS
authors: Silva
authors: Jerusa Simone
authors: JS
authors: Garcia
authors: Marcos N
authors: MN
authors: Eberlin
authors: Matthew James
authors: MJ
authors: Grossman
authors: Oswaldo Luiz
authors: OL
authors: Alves
authors: Lucia Regina
authors: LR
authors: Durrant
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