title: Polymorphisms of the CYP1A1 and CYP2E1 genes in head and neck squamous cell carcinoma risk.
abstract: Polymorphisms in genes that encode P450 cytochrome enzymes may increase carcinogen activation or decrease their inactivation and consequently, promote the development of cancer. The aims of this study were to identify the MspI-CYP1A1, PstI-CYP2E1 and DraI-CYP2E1 polymorphisms in patients with head and neck cancer and to compare with individuals without cancer; to evaluate the association of these polymorphisms with risk factors and clinical histopathological parameters. In the study group, 313 patients were evaluated for CYP1A1, 217 for CYP2E1 (PstI) and 211 for CYP2E1 (DraI) and in the control group 417, 334 and 374 individuals, respectively. Molecular analysis was performed by PCR-RFLP technique, and chi-square and multiple logistic regression tests were used for statistical analysis. The result of analysis regarding individuals evaluated for CYP1A1 (MspI) showed that age (OR: 8.15; 95% CI 5.57-11.92) and smoking (OR: 5.37; 95% CI 3.52-8.21) were predictors for the disease; for the CYP2E1 (PstI and DraI), there were associations with age (PstI-OR: 9.10; 95% CI 5.86-14.14/DraI-OR: 8.07; 95% CI 5.12-12.72), smoking (PstI-OR: 4.10; 95% CI 2.44-6.89/DraI-OR: 5.73; 95% CI 3.34-9.82), alcohol (PstI-OR: 1.93; 95% CI 1.18-3.16/DraI-OR: 1.69; 95% CI 1.02-2.81), respectively, with disease development. CYP2E1 (PstI) was less frequent in patient group (OR: 0.48; 95% CI 0.23-0.98). Regarding clinical histopathological parameters, CYP1A1 polymorphism was less frequent in the larynx primary anatomic site (OR = 0.45; 95% CI = 0.28-0.73; P = 0.014). In conclusion, we confirm that age, smoking and alcohol consumption are risk factors for this disease and the polymorphisms investigated have no association with the development of head and neck cancer.
authors: Genetics and Molecular Biology Research Unit-UPGEM, São José do Rio Preto Medical School (FAMERP), São José do Rio Preto, SP, Brazil.
authors: Nathália Moreno
authors: NM
authors: Cury
authors: Anelise
authors: A
authors: Russo
authors: Ana Lívia Silva
authors: AL
authors: Galbiatti
authors: Mariângela Torreglosa
authors: MT
authors: Ruiz
authors: Luiz Sérgio
authors: LS
authors: Raposo
authors: José Victor
authors: JV
authors: Maniglia
authors: Erika Cristina
authors: EC
authors: Pavarino
authors: Eny Maria
authors: EM
authors: Goloni-Bertollo
references: Fertil Steril. 2001 Dec;76(6):1202-6
references: 11730751
references: Chin Med J (Engl). 2007 Oct 20;120(20):1797-802
references: 18028774
references: Expert Opin Ther Targets. 2007 Feb;11(2):207-17
references: 17227235
references: Nucleic Acids Res. 1988 Feb 11;16(3):1215
references: 3344216
references: Oral Oncol. 2001 Apr;37(3):205-10
references: 11287272
references: Int J Cancer. 2000 Sep 1;87(5):734-40
references: 10925369
references: Yonsei Med J. 2007 Apr 30;48(2):233-9
references: 17461521
references: J Biochem Mol Toxicol. 2007;21(4):163-8
references: 17936929
references: Head Neck. 2004 Jan;26(1):63-70
references: 14724908
references: Cancer Res. 1992 Dec 1;52(23):6712-5
references: 1423319
references: Mutat Res. 2005 Jul 1;574(1-2):112-23
references: 15914211
references: J Clin Pathol. 1998 Apr;51(4):294-8
references: 9659241
references: Pharmacogenetics. 2000 Mar;10(2):105-14
references: 10761998
references: J Oral Pathol Med. 2005 Sep;34(8):473-7
references: 16091114
references: Curr Opin Otolaryngol Head Neck Surg. 2005 Aug;13(4):207-11
references: 16012243
references: J Hum Genet. 2005;50(12):618-27
references: 16228113
references: World J Gastroenterol. 2007 Feb 21;13(7):997-1002
references: 17373732
references: Arh Hig Rada Toksikol. 2009 Jun;60(2):217-42
references: 19581216
references: Curr Opin Oncol. 2009 May;21(3):194-200
references: 19363341
references: Oral Oncol. 2009 Sep;45(9):e73-9
references: 19442564
references: Cancer Res. 2000 Jun 15;60(12):3155-9
references: 10866304
references: J Gerontol A Biol Sci Med Sci. 2007 Apr;62(4):361-6
references: 17452728
references: Int J Cancer. 2003 May 1;104(5):650-7
references: 12594823
references: Postepy Hig Med Dosw (Online). 2008 Sep 23;62:490-501
references: 18833034
references: Mol Biol Rep. 2011 Feb;38(2):1071-8
references: 20661649
references: BMC Cancer. 2001;1:4
references: 11389775
references: J Toxicol Environ Health A. 2008;71(13-14):887-97
references: 18569591
references: Fertil Steril. 2005 Aug;84(2):446-53
references: 16084888
references: Int J Epidemiol. 2003 Feb;32(1):60-3
references: 12690010
references: J Natl Cancer Inst. 1997 Aug 20;89(16):1207-12
references: 9274915
references: Pharmacogenetics. 1998 Apr;8(2):91-100
references: 10022746
references: J Craniomaxillofac Surg. 2007 Mar;35(2):120-5
references: 17449259
references: J Natl Cancer Inst. 2007 May 16;99(10):777-89
references: 17505073
references: Lancet Oncol. 2006 Feb;7(2):149-56
references: 16455479
references: Head Neck. 2008 Sep;30(9):1139-47
references: 18642288
references: Carcinogenesis. 1993 Jan;14(1):85-8
references: 8093864
references: Gynecol Oncol. 2001 May;81(2):263-9
references: 11330960
references: Oral Oncol. 2006 Jul;42(6):632-7
references: 16488179
references: Int J Oncol. 2008 May;32(5):945-73
references: 18425322
references: Eur J Cancer. 2007 Jul;43(10):1590-5
references: 17512722
references: Oral Oncol. 2001 Jul;37(5):437-45
references: 11377232
references: World J Gastroenterol. 2004 Dec 1;10(23):3389-93
references: 15526353
references: J Dent Res. 2007 Feb;86(2):104-14
references: 17251508
references: Head Neck. 2006 Sep;28(9):819-26
references: 16721740
references: FEBS Lett. 1990 Apr 9;263(1):131-3
references: 1691986
references: J Oral Pathol Med. 2006 Jan;35(1):11-8
references: 16393248
references: Cancer Causes Control. 1998 Jan;9(1):99-108
references: 9486469
references: World J Gastroenterol. 2007 Nov 21;13(43):5725-30
references: 17963298
references: Cancer Invest. 2009 Oct;27(8):869-76
references: 19639480
references: Braz J Med Biol Res. 2006 Dec;39(12):1569-74
references: 17160265
references: Mol Biol Rep. 2010 Dec;37(8):3833-9
references: 20217240
references: Oral Oncol. 2005 Mar;41(3):244-60
references: 15743687
references: BMC Cancer. 2009 Jun 16;9:187
references: 19531241
references: Otolaryngol Head Neck Surg. 2003 May;128(5):674-80
references: 12748560