title: Evaluating differentiation propensity of in-house derived human embryonic stem cell lines KIND-1 and KIND-2.
abstract: Human embryonic stem (hES) cells possess the ability to self-renew indefinitely and provide a potential source of differentiated progeny representing all three embryonic germ layers. Although hES cell lines share the expression of typical pluripotency markers, limited data is available regarding their differentiation capabilities. We have earlier reported the in-house derivation of two hES cell lines, KIND-1 and KIND-2 on human feeders. Here, we describe a comparative study carried out on both these cell lines to better understand the differentiation potential of KIND-1 and KIND-2 by gene expression analysis of representative gene transcripts reflecting pluripotency and the three germ layers viz. ectoderm, mesoderm, and endoderm. Gene expression analysis and immunolocalization studies were undertaken on (a) 7- and 14-d old embryoid bodies (EBs) (b) spontaneously differentiated cells from EBs, (c) cells derived from EBs under the influence of various growth factor treatments and (d) KIND-1 and KIND-2 cells co-cultured on mouse embryonic visceral endoderm-like feeder (END-2). Despite both the cell lines being XX, derived, passaged, and cultured similarly, KIND-1 exhibits preferential differentiation towards endodermal lineage whereas KIND-2 spontaneously forms beating cardiomyocytes. Perhaps the occurrence of discrete epigenetic profile in both the cell lines predisposes them to encompass different developmental potential in vitro. Our data provide evidence for existence of distinct differentiation propensity among hES cell lines and emphasizes the need to derive more hES cell lines for future regenerative medicine.
authors: Stem Cell Biology Department, National Institute for Research in Reproductive Health, Parel, Mumbai, 400 012, India.
authors: Punam
authors: P
authors: Nagvenkar
authors: Prasad
authors: P
authors: Pethe
authors: Harsha
authors: H
authors: Pawani
authors: Jyoti
authors: J
authors: Telang
authors: Neeraj
authors: N
authors: Kumar
authors: Indira
authors: I
authors: Hinduja
authors: Kusum
authors: K
authors: Zaveri
authors: Deepa
authors: D
authors: Bhartiya
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