title: Detection of microspotted carcinoembryonic antigen on a glass substrate by scanning electrochemical microscopy.
abstract: Microspots of carbinoembryonic antigen (CEA) on glass substrates were characterized by scanning electrochemical microscopy (SECM). CEA was immobilized via a sandiwch method using horseradish peroxidase (HRP)-labeled anti-CEA. The reduction current of the oxidized form of ferrocenylmethanol generated by the HRP reaction was monitored to view SECM images. This method detects as low as ∼10(4) CEA molecules in a single 20-μm-radius spot.
authors: Department of Applied Chemistry, Graduate School of Engineering, Tohoku University, Sendai 980-77, Japan.
authors: H
authors: H
authors: Shiku
authors: T
authors: T
authors: Matsue
authors: I
authors: I
authors: Uchida