title: Study of GPCR-protein interactions using gel overlay assays and glutathione-S-transferase-fusion protein pull-downs.
abstract: Numerous recent studies have suggested that the predicted cytosolic domains of G protein-coupled receptors represent a surface for association with proteins that may serve multiple roles in receptor localization, turnover, and signaling beyond the well-characterized interactions of these receptors with heterotrimeric G proteins. This Chapter describes two in vitro methods for ascertaining interactions between G protein-coupled receptors and various binding partners: gel overlay strategies and GST-fusion protein pull-downs.
authors: Department of Pharmacology, Vanderbilt University Medical Center, Nashville, TN, USA.
authors: Ashley E
authors: AE
authors: Brady
authors: Yunjia
authors: Y
authors: Chen
authors: Lee E
authors: LE
authors: Limbird
authors: Qin
authors: Q
authors: Wang