title: Efficient direct extended-spectrum β-lactamase detection by multiplex real-time PCR: accurate assignment of phenotype by use of a limited set of genetic markers.
abstract: The number and diversity of genes potentially complicate genetic approaches to the rapid detection of transmissible extended-spectrum β-lactamase genes. We developed a robust multiplexed real-time PCR assay based on targets identified in a prior survey and used this to detect relevant genes in 617 consecutive clinical isolates of extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae.
authors: CIDM, Level 3 ICPMR Building, Westmead Hospital, Westmead NSW 2145, Australia.
authors: Justin
authors: J
authors: Ellem
authors: Sally R
authors: SR
authors: Partridge
authors: Jonathan R
authors: JR
authors: Iredell
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