title: [Immunogenicity of plasmid DNA and adenoviral vectors encoding HIV-1 subtype B env gene].
abstract: OBJECTIVE
  To construct DNA and recombinant adenovirus vector vaccines containing an env gene from the prevalent subtype B strain in China and try to use them for therapeutic and prophylactic vaccines.
  
  
  METHODS
  The candidate plasmid DNA vaccine pVR-gp160 and recombinant adenovirus vaccine rAdV-gp160 were constructed separately. BALB/c mice were immunized with these two vaccines in different administration schemes. HIV-1 Gp120-specific cellular responses and antibody levels were detected by ELISPOT and ELISA respectively.
  
  
  RESULTS
  DNA vaccine alone and combined vaccines in a DNA prime/rAdV-gp160 boost vaccination regimen induced high level of Gp120-specific cellular responses. While low level of Gp120-specific antibodies were elicited in all groups.
  
  
  CONCLUSIONS
  DNA and rAdV vaccines could efficiently express Gp160 protein and activate specific cellular responses.
authors: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 100052, China.
authors: Hai-Ru
authors: HR
authors: Yang
authors: Ling-Fei
authors: LF
authors: Zhang
authors: Xia
authors: X
authors: Feng
authors: Shuang-Qing
authors: SQ
authors: Yu
authors: Zhu-Lun
authors: ZL
authors: Zhuang
authors: Hong-Xia
authors: HX
authors: Li
authors: Yi
authors: Y
authors: Zeng