title: A 304 bp insertion/deletion mutation in promoter region induces the increase of porcine IDH3β gene expression.
abstract: Obese and lean pig breeds show obvious differences in adipose metabolism/fat deposition; however, the molecular mechanism underlying phenotype variation remains unknown. In order to understand it, we analyzed the differences of gene expression in backfat between Meishan (a typical Chinese indigenous obese breed) and Large White (a lean Western breed) pigs. Here, we cloned porcine β subunit of IDH3 (IDH3B) and 2447 bp 5'-flanking sequence of this gene, and determined the genomic structure. Porcine IDH3B contains three isoforms, IDH3B ( 1 ), IDH3B ( 2 ) and IDH3B ( 3 ). Real-time RT-PCR revealed that these three isoforms were prevalently up-regulated in backfat of western commercial pigs, Large White, Landrace and Duroc, compared with Chinese indigenous breeds, Meishan and Tongcheng pigs. A 304 bp insertion/deletion variant was found in the 5'-flanking region. Dual-luciferase reporter assays showed that in vitro the promoter of IDH3B gene with the insertion had higher luciferase activity as compared with the wild type. Three genotypes AA, AB and BB, due to this insertion, were detected, and the frequency of allele A was dominant in western commercial pigs, whereas allele B predominated in Chinese indigenous breeds. IDH3B mRNA expression in Meishan pigs was more abundant with genotype AA than with genotype AB or BB, as in Large White pigs. In addition, the polymorphism was detected in 317 pigs of a Large White × Meishan F2 resource population. Association analysis showed that pigs with genotype AA possessed higher backfat thickness at buttocks than those with genotype AB (P < 0.05) or BB. These data suggested that the 304 bp insertion mutation in promoter region increased the expression of porcine IDH3β transcripts and this mutation might be a candidate marker for marker assistant selection in swine breeding.
authors: Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture & Key Laboratory of Agriculture Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science, Huazhong Agricultural University, Wuhan 430070, China.
authors: Zhuqing
authors: Z
authors: Ren
authors: Wanghong
authors: W
authors: Liu
authors: Rong
authors: R
authors: Zheng
authors: Buo
authors: B
authors: Zuo
authors: Dequan
authors: D
authors: Xu
authors: Minggang
authors: M
authors: Lei
authors: Fenge
authors: F
authors: Li
authors: Jialian
authors: J
authors: Li
authors: Debin
authors: D
authors: Ni
authors: Yuanzhu
authors: Y
authors: Xiong
references: J Biol Chem. 2002 Nov 8;277(45):43454-62
references: 12207025
references: J Biol Chem. 2003 Dec 5;278(49):49323-31
references: 14512428
references: Proc Natl Acad Sci U S A. 1978 Oct;75(10):4853-7
references: 283395
references: Biochemistry. 1972 Apr 11;11(8):1501-8
references: 4336621
references: Sci China C Life Sci. 2006 Aug;49(4):342-8
references: 16989279
references: J Biol Chem. 1999 Dec 24;274(52):36866-75
references: 10601238
references: J Biol Chem. 1980 Sep 25;255(18):8859-64
references: 7410398
references: Methods. 2001 Dec;25(4):402-8
references: 11846609
references: Genomics. 1995 Feb 10;25(3):674-81
references: 7759102
references: Nucleic Acids Res. 1994 Nov 11;22(22):4673-80
references: 7984417
references: BMC Mol Biol. 2006 May 09;7:17
references: 16684343
references: J Biol Chem. 2003 Dec 26;278(52):52146-53
references: 14555658