title: Nipah virus uses leukocytes for efficient dissemination within a host.
abstract: Nipah virus (NiV) is a recently emerged zoonotic paramyxovirus whose natural reservoirs are several species of Pteropus fruit bats. NiV provokes a widespread vasculitis often associated with severe encephalitis, with up to 75% mortality in humans. We have analyzed the pathogenesis of NiV infection, using human leukocyte cultures and the hamster animal model, which closely reproduces human NiV infection. We report that human lymphocytes and monocytes are not permissive for NiV and a low level of virus replication is detected only in dendritic cells. Interestingly, despite the absence of infection, lymphocytes could efficiently bind NiV and transfer infection to endothelial and Vero cells. This lymphocyte-mediated transinfection was inhibited after proteolytic digestion and neutralization by NiV-specific antibodies, suggesting that cells could transfer infectious virus to other permissive cells without the requirement for NiV internalization. In NiV-infected hamsters, leukocytes captured and carried NiV after intraperitoneal infection without themselves being productively infected. Such NiV-loaded mononuclear leukocytes transfer lethal NiV infection into naïve animals, demonstrating efficient virus transinfection in vivo. Altogether, these results reveal a remarkable capacity of NiV to hijack leukocytes as vehicles to transinfect host cells and spread the virus throughout the organism. This mode of virus transmission represents a rapid and potent method of NiV dissemination, which may contribute to its high pathogenicity.
authors: INSERM, U758, Ecole Normale Supérieure de Lyon, Lyon, F-69007 France.
authors: Cyrille
authors: C
authors: Mathieu
authors: Christine
authors: C
authors: Pohl
authors: Judit
authors: J
authors: Szecsi
authors: Selena
authors: S
authors: Trajkovic-Bodennec
authors: Séverine
authors: S
authors: Devergnas
authors: Hervé
authors: H
authors: Raoul
authors: François-Loïc
authors: FL
authors: Cosset
authors: Denis
authors: D
authors: Gerlier
authors: T Fabian
authors: TF
authors: Wild
authors: Branka
authors: B
authors: Horvat
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