title: Charge state-selective separation of peptides by reversible modification of amino groups and strong cation-exchange chromatography: evaluation in proteomic studies using peptide-centric database searches.
abstract: Here we describe an integrated approach for the selective separation of peptides from complex mixtures using strong cation-exchange chromatography. The procedure exploits the charge differences produced by reversible modification of primary amino groups in peptides, enabling their separation into three major fractions: 1) neutral peptides 2) peptides with one positive charge and 3) peptides with 2 or more positive charges. The procedure demonstrated an excellent selectivity which allowed restricted MS/MS ion searches with peptide-centric databases.
authors: Center for Genetic Engineering and Biotechnology, Cubanacán, Habana, Cuba. lazaro.betancourt@cigb.edu.cu
authors: Lázaro H
authors: LH
authors: Betancourt
authors: Aniel
authors: A
authors: Sánchez
authors: Yasset
authors: Y
authors: Pérez
authors: Jorge
authors: J
authors: Fernandez de Cossio
authors: Jeovanis
authors: J
authors: Gil
authors: Patricia
authors: P
authors: Toledo
authors: Seiji
authors: S
authors: Iguchi
authors: Saburo
authors: S
authors: Aimoto
authors: Luis J
authors: LJ
authors: González
authors: Gabriel
authors: G
authors: Padrón
authors: Toshifumi
authors: T
authors: Takao
authors: Vladimir
authors: V
authors: Besada