title: Integrated approach to produce a recombinant, His-tagged human α-galactosidase A in mammalian cells.
abstract: Successful production of recombinant proteins (r-proteins) by transient gene expression (TGE) depends on several parameters (including producer cells, culture conditions, transfection procedure, or expression vector) that should be optimized when producing any recombinant product. In this work, TGE-based production of human α-galactosidase A (GLA) is described. Producer cells, expression vectors, and parameters influencing cell metabolism after transfection have been tested. The enzyme is secreted, has the right molecular weight, and is enzymatically active. Productivities of up to 30-40 mg/L have been achieved, with a simple, fast procedure. A 6 × His tag allows enzyme purification in a single step, rendering a highly pure product. We propose a TGE-based protocol able to produce up to several milligrams per liter of highly pure, active GLA in a time as short as a few days. By this, enough amounts of engineered versions of the enzyme can be easily produced to be tested in vitro or in preclinical trials.
authors: CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Barcelona, Spain. jlcorchero@ciber-bbn.es
authors: José Luis
authors: JL
authors: Corchero
authors: Rosa
authors: R
authors: Mendoza
authors: Julia
authors: J
authors: Lorenzo
authors: Victor
authors: V
authors: Rodríguez-Sureda
authors: Carmen
authors: C
authors: Domínguez
authors: Esther
authors: E
authors: Vázquez
authors: Neus
authors: N
authors: Ferrer-Miralles
authors: Antonio
authors: A
authors: Villaverde