title: Molecular epidemiology and phylogeny reveal complex spatial dynamics in areas where canine parvovirus is endemic.
abstract: Canine parvovirus type 2 (CPV-2) is a severe enteric pathogen of dogs, causing high mortality in unvaccinated dogs. After emerging, CPV-2 spread rapidly worldwide. However, there is now some evidence to suggest that international transmission appears to be more restricted. In order to investigate the transmission and evolution of CPV-2 both nationally and in relation to the global situation, we have used a long-range PCR to amplify and sequence the full VP2 gene of 150 canine parvoviruses obtained from a large cross-sectional sample of dogs presenting with severe diarrhea to veterinarians in the United Kingdom, over a 2-year period. Among these 150 strains, 50 different DNA sequence types (S) were identified, and apart from one case, all appeared unique to the United Kingdom. Phylogenetic analysis provided clear evidence for spatial clustering at the international level and for the first time also at the national level, with the geographical range of some sequence types appearing to be highly restricted within the United Kingdom. Evolution of the VP2 gene in this data set was associated with a lack of positive selection. In addition, the majority of predicted amino acid sequences were identical to those found elsewhere in the world, suggesting that CPV VP2 has evolved a highly fit conformation. Based on typing systems using key amino acid mutations, 43% of viruses were CPV-2a, and 57% CPV-2b, with no type 2 or 2c found. However, phylogenetic analysis suggested complex antigenic evolution of this virus, with both type 2a and 2b viruses appearing polyphyletic. As such, typing based on specific amino acid mutations may not reflect the true epidemiology of this virus. The geographical restriction that we observed both within the United Kingdom and between the United Kingdom and other countries, together with the lack of CPV-2c in this population, strongly suggests the spread of CPV within its population may be heterogeneously subject to limiting factors. This cross-sectional study of national and global CPV phylogeographic segregation reveals a substantially more complex epidemic structure than previously described.
authors: Faculty of Health and Life Science, School of Veterinary Science, University of Liverpool, Leahurst Campus, Chester High Road, Neston, South Wirral CH64 7TE, United Kingdom. s.r.clegg@liv.ac.uk
authors: S R
authors: SR
authors: Clegg
authors: K P
authors: KP
authors: Coyne
authors: J
authors: J
authors: Parker
authors: S
authors: S
authors: Dawson
authors: S A
authors: SA
authors: Godsall
authors: G
authors: G
authors: Pinchbeck
authors: P J
authors: PJ
authors: Cripps
authors: R M
authors: RM
authors: Gaskell
authors: A D
authors: AD
authors: Radford
references: Bioinformatics. 2005 May 15;21(10):2531-3
references: 15713735
references: J Virol Methods. 1998 Aug;73(2):197-200
references: 9766890
references: J Gen Virol. 2001 Dec;82(Pt 12):3021-3025
references: 11714979
references: Nature. 2008 May 29;453(7195):615-9
references: 18418375
references: Virology. 2009 Mar 1;385(1):5-10
references: 19144369
references: Syst Biol. 2003 Oct;52(5):696-704
references: 14530136
references: PLoS Pathog. 2008 Apr 18;4(4):e1000050
references: 18421380
references: Mol Biol Evol. 2007 Aug;24(8):1596-9
references: 17488738
references: Vet Res Commun. 2005 Nov;29(8):735-43
references: 16369887
references: J Mol Biol. 1990 Oct 5;215(3):403-10
references: 2231712
references: Virus Genes. 2005 Oct;31(2):171-4
references: 16025242
references: Vet Rec. 1996 May 18;138(20):495-6
references: 8736503
references: J Virol. 2006 Sep;80(17):8482-92
references: 16912298
references: J Virol. 2007 Feb;81(4):1961-71
references: 17151126
references: Vet Microbiol. 1999 Sep 1;69(1-2):47-50
references: 10515268
references: Infect Genet Evol. 2008 May;8(3):239-46
references: 17921073
references: J Vet Med B Infect Dis Vet Public Health. 2006 Dec;53(10):468-72
references: 17123424
references: Virology. 1986 Jan 15;148(1):121-32
references: 3942033
references: Vet Rec. 1995 Feb 18;136(7):174-5
references: 7762129
references: J Virol. 1988 Jan;62(1):266-76
references: 2824850
references: BMC Evol Biol. 2007 Nov 08;7:214
references: 17996036
references: Vet Rec. 2008 Nov 1;163(18):543-4
references: 18978368
references: Mol Biol Evol. 2001 Jun;18(6):1001-13
references: 11371589
references: Bioinformatics. 2009 Jan 1;25(1):126-7
references: 18984599
references: Virology. 1988 Mar;163(1):230-2
references: 3348000
references: Vet Microbiol. 2000 Jul 31;75(2):127-33
references: 10889403
references: Vet Microbiol. 2008 Apr 1;128(1-2):48-55
references: 18006253
references: J Clin Microbiol. 2007 Dec;45(12):4044-7
references: 17928423
references: Proc Natl Acad Sci U S A. 2005 Jan 11;102(2):379-84
references: 15626758
references: Vet Rec. 1979 Aug 25;105(8):156-9
references: 516347
references: Mol Biol Evol. 1993 May;10(3):512-26
references: 8336541
references: Aust Vet J. 1978 Dec;54(12):593
references: 753224
references: Microbiol Rev. 1990 Sep;54(3):316-29
references: 2215424
references: Emerg Infect Dis. 2007 Aug;13(8):1222-4
references: 17953097
references: Vet Rec. 2010 Aug 7;167(6):196-201
references: 20693502
references: J Gen Virol. 2008 Sep;89(Pt 9):2280-2289
references: 18753238
references: Aust Vet J. 2007 Oct;85(10):392-6
references: 17903125
references: J Virol Methods. 1995 Nov;55(3):427-33
references: 8609207
references: J Vet Diagn Invest. 2005 Mar;17(2):133-8
references: 15825493
references: J Virol. 1997 Dec;71(12):9214-22
references: 9371580
references: Vet Microbiol. 2005 Jan 5;105(1):19-28
references: 15607080