title: Rabbit anti-rabies immunoglobulins production and evaluation.
abstract: Due to the disadvantages of human and equine rabies immunoglobulin, it is necessary to develop a substitute for HRIG and ERIG, especially for those people living in the developing countries. Because of higher affinity and lower immunogenicity of rabbit's immunoglobulins, anti-rabies immunoglobulins specific to rabies virus were produced in rabbits as a bioreactor, and had been characterized by ELISA, affinity assay, immunofluorescence assay (IFA), immunocytochemistry, rapid fluorescent focus inhibition test (RFFIT). ELISA, affinity assay and IFA showed that rabbit RIG (RRIG) bound specifically to rabies virions. RFFIT result showed that RRIG has neutralization activity. This result was confirmed in vivo in a Kunming mouse challenge model and the protection rate of the treatment with RRIG was higher (25%) than that offered by HRIG when mice were challenged with a lethal RV dose. Our results demonstrate that RRIG is safe and efficacious as a candidate drug to replace rabies immunoglobulin in post-exposure prophylaxis.
authors: Key Laboratory of Antibody Technique of Ministry of Health, Nanjing Medical University, No. 140, Hanzhong Road, Nanjing 210029, People's Republic of China.
authors: Xinjian
authors: X
authors: Liu
authors: Qiongqiong
authors: Q
authors: Liu
authors: Xiaomin
authors: X
authors: Feng
authors: Qi
authors: Q
authors: Tang
authors: Zhongcan
authors: Z
authors: Wang
authors: Suqing
authors: S
authors: Li
authors: Zhenqing
authors: Z
authors: Feng
authors: Jin
authors: J
authors: Zhu
authors: Xiaohong
authors: X
authors: Guan