title: Genotype of the LMNA 1908C>T variant is associated with generalized obesity in Asian Indians in North India.
abstract: OBJECTIVE
  To determine the frequency distribution of LMNA 1908C>T SNP and its association with generalized obesity, abdominal obesity and coexistent metabolic disorders in nondiabetic Asian Indians living in a metropolitan city of north India.
  
  
  METHODS
  A cross-sectional population-based study of LMNA 1908C>T polymorphism with obesity and insulin resistance as outcome.
  
  
  METHODS
  Five hundred and fifty-one Asian Indians, with 240 obese and 289 nonobese subjects.
  
  
  METHODS
  Allelic and genotypic frequency of LMNA 1908C>T were determined by PCR-RFLP. Association of LMNA alleles and genotypes was analysed with various measures of obesity [BMI ≥ 25 kg/m(2) , percentage body fat (by DEXA); subcutaneous and intra-abdominal fat at L2-3 level by single slice MRI in a subsample and surrogate marker of insulin resistance (fasting serum insulin levels >10 μU/ml in men and >11 μU/ml in women).
  
  
  RESULTS
  Forty-six per cent of the subjects had generalized obesity while 54% had abdominal obesity. Frequency of C and T alleles was 0·71 and 0·29, respectively. Higher frequency of variant allele (T) was observed in obese than nonobese individuals (P = 0·001). On multivariate analysis adjusting for age, gender and serum insulin levels, subjects with LMNA1908T/T genotype were at 5·6 times higher risk [OR (95% CI): 5·6 (2·5-12·2), P = 0·001], while individuals with genotypes with at least one T allele, i.e. 1908C/T and T/T genotypes, were at 2·7 times higher odds to develop generalized obesity [OR (95% CI): 2·7 (1·8-4·1), P = 0·001].
  
  
  CONCLUSIONS
  LMNA 1908T/T and C/T genotypes emerged as independent genetic risk factors for generalized obesity in Asian north Indians.
authors: Department of Biochemistry, All India Institute of Medical Sciences, New Delhi, India.
authors: Mukti
authors: M
authors: Sharma
authors: Anoop
authors: A
authors: Misra
authors: Naval
authors: N
authors: Vikram
authors: Bhatt
authors: B
authors: Suryaprakash
authors: Shivani
authors: S
authors: Chhabra
authors: Neha
authors: N
authors: Garg
authors: Ravindra Mohan
authors: RM
authors: Pandey
authors: Ashish Datt
authors: AD
authors: Upadhyay
authors: Kalpana
authors: K
authors: Luthra