title: Plasma membrane localization of the type I H(+)-PPase AVP1 in sieve element-companion cell complexes from Arabidopsis thaliana.
abstract: Previous literature has shown the presence of a plasma membrane (PM) localized type I H(+)-PPase in sieve elements of Ricinus communis. Unfortunately, the physiological relevance of these findings remains obscure due to the lack of genetic and molecular reagents to study R. communis. The availability of H(+)-PPase gain and loss-of-function mutants in Arabidopsis thaliana makes this plant an attractive genetic model to address the question, but data on the PM localization of this H(+)-PPase in A. thaliana are limited to two proteomic approaches. Here we present the first report on the localization of the type I H(+)-PPase AVP1 in sieve element-companion cell complexes (SE-CCc) from A. thaliana. Double epifluorescence and immunogold labeling experiments are consistent with the co-localization of AVP1 and PIP1 (a bona fide PM maker) in PM of SE-CCc from A. thaliana.
authors: School of Life Sciences, Arizona State University, Tempe, AZ 85287-1501, USA.
authors: Julio
authors: J
authors: Paez-Valencia
authors: Araceli
authors: A
authors: Patron-Soberano
authors: Alejandra
authors: A
authors: Rodriguez-Leviz
authors: Jonathan
authors: J
authors: Sanchez-Lares
authors: Concepcion
authors: C
authors: Sanchez-Gomez
authors: Pedro
authors: P
authors: Valencia-Mayoral
authors: Guadalupe
authors: G
authors: Diaz-Rosas
authors: Roberto
authors: R
authors: Gaxiola