title: Development of SSR markers from Citrus clementina (Rutaceae) BAC end sequences and interspecific transferability in Citrus.
abstract: OBJECTIVE
  Microsatellite primers were developed from bacterial artificial chromosome (BAC) end sequences of Citrus clementina and their transferability and polymorphism tested in the genus Citrus for future anchorage of physical and genetic maps and comparative interspecific genetic mapping. •
  
  
  RESULTS
  Using PAGE and DNA silver staining, 79 primer pairs were selected for their transferability and polymorphism among 526 microsatellites mined in BES. A preliminary diversity study in Citrus was conducted with 18 of them, in C. reticulata, C. maxima, C. medica, C. sinensis, C. aurantium, C. paradisi, C. lemon, C. aurantifolia, and some papedas (wild citrus), using a capillary electrophoresis fragment analyzer. Intra- and interspecific polymorphism was observed, and heterozygous markers were identified for the different genotypes to be used for genetic mapping. •
  
  
  CONCLUSIONS
  These results indicate the utility of the developed primers for comparative mapping studies and the integration of physical and genetic maps.
authors: Centro de Protección Vegetal y Biotecnología, IVIA, Apartado Oficial 46113 Moncada (Valencia), Spain.
authors: Frédérique
authors: F
authors: Ollitrault
authors: Javier
authors: J
authors: Terol
authors: Jose Antonio
authors: JA
authors: Pina
authors: Luis
authors: L
authors: Navarro
authors: Manuel
authors: M
authors: Talon
authors: Patrick
authors: P
authors: Ollitrault