title: A generic real-time TaqMan assay for specific detection of lapinized Chinese vaccines against classical swine fever.
abstract: Classical swine fever (CSF) is a highly contagious disease, causing severe economic losses in the pig industry worldwide. Vaccination of pigs with lapinized Chinese vaccines is still practised in some regions of the world, where the virus is enzootic, in order to prevent and control the disease. However, a single real-time assay that can detect all lapinized Chinese vaccines used widely, namely, Lapinized Philippines Coronel (LPC), Hog Cholera Lapinized virus (HCLV) and the Riems C-strain is still lacking. This study describes a real-time RT-PCR assay, targeting the N(pro) gene region, for specific detection of these lapinized vaccine strains. The assay is highly sensitive, with a detection limit of 10 genome copies per reaction for HCLV and Riems C-strain and highly specific, as more than 100 strains of wild type CSFV representing all major genotypes were not detected. The assay is also highly repeatable: the coefficient of variation of Ct values in three runs was 2.77% for the detection of 10 copies of the vaccine viral RNA. This study provides a potentially useful tool for specific detection of the lapinized Chinese vaccines, HCLV and C-strain, and the differentiation of these vaccines from wild type CSFV.
authors: Joint R&D Division in Virology, The National Veterinary Institute & The Swedish University of Agricultural Sciences, OIE Collaborating Centre for Biotechnology-based Diagnosis of Infectious Diseases in Veterinary Medicine, 751 89 Uppsala, Sweden. Lihong.Liu@sva.se
authors: Lihong
authors: L
authors: Liu
authors: Hongyan
authors: H
authors: Xia
authors: Helen
authors: H
authors: Everett
authors: Olubukola
authors: O
authors: Sosan
authors: Helen
authors: H
authors: Crooke
authors: Alexandra
authors: A
authors: Meindl-Böhmer
authors: Hua-Ji
authors: HJ
authors: Qiu
authors: Volker
authors: V
authors: Moennig
authors: Sándor
authors: S
authors: Belák
authors: Frederik
authors: F
authors: Widén