title: Development and characterization of microsatellite markers for Sagittaria trifolia var. sinensis (Alismataceae).
abstract: OBJECTIVE
  Microsatellite markers were developed for the aquatic plant Sagittaria trifolia var. sinensis to assess its genetic diversity and population structure. Cross-species transferability was assayed in eight congeneric species.
  
  
  RESULTS
  Seventeen microsatellite markers were isolated and characterized in Sagittaria trifolia var. sinensis using Fast Isolation by AFLP of Sequence COntaining Repeats (FIASCO) protocol. Across the evaluated populations, 14 of the markers showed polymorphisms with 3 to 11 alleles per locus; the observed and expected heterozygosity (H(o) and H(E)) ranged from 0.0000 to 0.6364 and from 0.0000 to 0.8386, respectively. Nine of the loci were successfully amplified in the congeneric species.
  
  
  CONCLUSIONS
  These markers will be useful for further investigation of population genetics in Sagittaria trifolia var. sinensis and related research in Sagittaria species.
authors: Key Laboratory of MOE for Plant Development Biology, Department of Genetics, College of Life Sciences, Wuhan University, Wuhan 430072, P. R. China.
authors: Zhi-Hua
authors: ZH
authors: Wu
authors: Shu-Zhen
authors: SZ
authors: Wang
authors: Ji-Hong
authors: JH
authors: Hu
authors: Feng
authors: F
authors: Li
authors: Wei-Dong
authors: WD
authors: Ke
authors: Yi
authors: Y
authors: Ding