title: S-adenosyl-L-methionine activates actinorhodin biosynthesis by increasing autophosphorylation of the Ser/Thr protein kinase AfsK in Streptomyces coelicolor A3(2).
abstract: S-Adenosyl-L-methionine (SAM) is one of the major methyl donors in all living organisms. The exogenous treatment with SAM leads to increased actinorhodin production in Streptomyces coelicolor A3(2). In this study, mutants from different stages of the AfsK-AfsR signal transduction cascade were used to test the possible target of SAM. SAM had no significant effect on actinorhodin production in afsK, afsR, afsS, or actII-open reading frame 4 (ORF4) mutant. This confirms that afsK plays a critical role in delivering the signal generated by exogenous SAM. The afsK-pHJL-KN mutant did not respond to SAM, suggesting the involvement of the C-terminal of AfsK in binding with SAM. SAM increased the in vitro autophosphorylation of kinase AfsK in a dose-dependent manner, and also abolished the effect of decreased actinorhodin production by a Ser/Thr kinase inhibitor, K252a. In sum, our results suggest that SAM activates actinorhodin biosynthesis in S. coelicolor M130 by increasing the phosphorylation of protein kinase AfsK.
authors: Division of Bioscience and Bioinformatics, College of Natural Science, Myongji University, Gyeonggi-Do, Republic of Korea.
authors: Ying-Yu
authors: YY
authors: Jin
authors: Jinhua
authors: J
authors: Cheng
authors: Seung Hwan
authors: SH
authors: Yang
authors: Lingzhu
authors: L
authors: Meng
authors: Sasikumar Arunachalam
authors: SA
authors: Palaniyandi
authors: Xin-Qing
authors: XQ
authors: Zhao
authors: Joo-Won
authors: JW
authors: Suh