title: MicroRNA-98 negatively regulates IL-10 production and endotoxin tolerance in macrophages after LPS stimulation.
abstract: Interleukin 10 (IL-10) is a potent anti-inflammatory cytokine that is crucial for dampening the inflammatory response after pathogen invasion, and was found to be produced by macrophages after exposure to lipopolysaccharide (LPS). It remains unclear whether microRNA-mediated regulatory mechanism is involved in LPS-induced IL-10 production. Here we reported that miR-98 expression in macrophages significantly decreased following LPS stimulation. We also found that miR-98 targets the 3'untranslated region of IL-10 transcript. Overexpression of miR-98 inhibited TLR4-triggered IL-10 production and promoted COX-2 expression. We further demonstrated that miR-98 significantly mitigated the induction of endotoxin tolerance, suggesting that miR-98-mediated posttranscriptional control could potentially be involved in fine tuning the critical level of IL-10 production in endotoxin tolerance.
authors: Institute of Immunology, Zhejiang University School of Medicine, Hangzhou, China.
authors: Yang
authors: Y
authors: Liu
authors: Qingyun
authors: Q
authors: Chen
authors: Yinjing
authors: Y
authors: Song
authors: Lihua
authors: L
authors: Lai
authors: Jianli
authors: J
authors: Wang
authors: Hai
authors: H
authors: Yu
authors: Xuetao
authors: X
authors: Cao
authors: Qingqing
authors: Q
authors: Wang