title: Design and performance of the CDC real-time reverse transcriptase PCR swine flu panel for detection of 2009 A (H1N1) pandemic influenza virus.
abstract: Swine influenza viruses (SIV) have been shown to sporadically infect humans and are infrequently identified by the Influenza Division of the Centers for Disease Control and Prevention (CDC) after being received as unsubtypeable influenza A virus samples. Real-time reverse transcriptase PCR (rRT-PCR) procedures for detection and characterization of North American lineage (N. Am) SIV were developed and implemented at CDC for rapid identification of specimens from cases of suspected infections with SIV. These procedures were utilized in April 2009 for detection of human cases of 2009 A (H1N1) pandemic (pdm) influenza virus infection. Based on genetic sequence data derived from the first two viruses investigated, the previously developed rRT-PCR procedures were optimized to create the CDC rRT-PCR Swine Flu Panel for detection of the 2009 A (H1N1) pdm influenza virus. The analytical sensitivity of the CDC rRT-PCR Swine Flu Panel was shown to be 5 copies of RNA per reaction and 10(-1.3 - -0.7) 50% infectious doses (ID(50)) per reaction for cultured viruses. Cross-reactivity was not observed when testing human clinical specimens or cultured viruses that were positive for human seasonal A (H1N1, H3N2) and B influenza viruses. The CDC rRT-PCR Swine Flu Panel was distributed to public health laboratories in the United States and internationally from April 2009 until June 2010. The CDC rRT-PCR Swine Flu Panel served as an effective tool for timely and specific detection of 2009 A (H1N1) pdm influenza viruses and facilitated subsequent public health response implementation.
authors: Virus Surveillance and Diagnostic Branch, Influenza Division, Centers for Disease Control and Prevention, Atlanta, Georgia, USA. SLindstrom@cdc.gov
authors: Bo
authors: B
authors: Shu
authors: Kai-Hui
authors: KH
authors: Wu
authors: Shannon
authors: S
authors: Emery
authors: Julie
authors: J
authors: Villanueva
authors: Roy
authors: R
authors: Johnson
authors: Erica
authors: E
authors: Guthrie
authors: LaShondra
authors: L
authors: Berman
authors: Christine
authors: C
authors: Warnes
authors: Nathelia
authors: N
authors: Barnes
authors: Alexander
authors: A
authors: Klimov
authors: Stephen
authors: S
authors: Lindstrom
references: Curr Protoc Microbiol. 2006 Dec;Chapter 15:Unit 15G.1
references: 18770580
references: Virology. 2008 Oct 25;380(2):304-11
references: 18768193
references: N Engl J Med. 2009 Jun 18;360(25):2616-25
references: 19423871
references: Virology. 1978 Jan;84(1):51-62
references: 74123
references: Emerg Infect Dis. 2008 Sep;14(9):1470-2
references: 18760023
references: Vet Microbiol. 2006 Dec 20;118(3-4):212-22
references: 16962262
references: Science. 1931 Feb 20;73(1886):214-5
references: 17729823
references: J Virol. 1989 Nov;63(11):4603-8
references: 2795713
references: Vet Microbiol. 2000 May 22;74(1-2):47-58
references: 10799777
references: Proc Natl Acad Sci U S A. 2007 Dec 26;104(52):20949-54
references: 18093945
references: J Infect Dis. 1977 Dec;136 Suppl:S363-8
references: 606760
references: Nature. 2009 Jun 25;459(7250):1122-5
references: 19516283
references: J Clin Microbiol. 1984 Oct;20(4):833-5
references: 6092435
references: Clin Infect Dis. 2007 Apr 15;44(8):1084-8
references: 17366454
references: Virus Res. 2004 Jul;103(1-2):67-73
references: 15163491
references: Nature. 2009 Jun 18;459(7249):931-9
references: 19525932
references: N Engl J Med. 2009 Jun 18;360(25):2605-15
references: 19423869
references: Emerg Infect Dis. 2004 Feb;10(2):311-6
references: 15030703
references: J Clin Microbiol. 2006 Mar;44(3):1123-6
references: 16517910
references: MMWR Morb Mortal Wkly Rep. 2010 Jul 30;59(29):901-8
references: 20671661
references: J Virol. 1994 Apr;68(4):2051-8
references: 8138990
references: Science. 2010 Jun 18;328(5985):1529
references: 20558710
references: Science. 2009 Jul 10;325(5937):197-201
references: 19465683
references: J Gen Virol. 1989 Dec;70 ( Pt 12):3297-303
references: 2558159
references: Virology. 2004 Oct 10;328(1):101-19
references: 15380362
references: MMWR Morb Mortal Wkly Rep. 2009 Apr 24;58(15):400-2
references: 19390508
references: Virus Res. 2000 Jun;68(1):71-85
references: 10930664
references: Clin Infect Dis. 2011 Jan 1;52 Suppl 1:S36-43
references: 21342897