title: DNA-ploidy and proliferative activity in uterine-tumors - an in-vivo study using bromodeoxyuridine and flow-cytometry.
abstract: We have studied the DNA ploidy and the proliferative activity in 102 patients with endometrial and cervical carcinoma, by flow cytometry. Samples were excised 1 hour after bromodeoxyuridine (BrdU, 250 mg/) e.v. infusion and fixed in 70% ethanol. Nuclear DNA content and BrdU incorporation, were simultaneously determined to obtain ploidy (DNA index) and proliferative activity (BrdU-labeling index, LI). No acute toxicity or side effects related to BrdU injection were recorded. The overall feasibility of the determinations was higher than 90% (93/102). Twenty-two out of 59 (37.2%) endometrial neoplasms and 23 out of 34 (67.6%) cervical neoplasms were aneuploid, with a median DNA-index of the aneuploid peak of 1.3 and 1.4, respectively. Overall median BrdU LIs were 4.8% and 7.2%. Proliferative activity was found to be higher in aneuploid tumors (p<.05). DNA ploidy and/or BrdU-LI were not significantly related either with the clinical stage or the histopathologic grading in either tumor type. The BrdU in vivo administration coupled with bivariate FCM for measurement is a simple method that can be performed in clinical settings to better evaluate the prognostic significance of proliferative parameters in gynecological tumors.
authors: UNIV PAVIA,MED CLIN 2,I-27100 PAVIA,ITALY. UNIV PAVIA,OSTET GINECOL CLIN,I-27100 PAVIA,ITALY. IRCCS SAN MATTEO,PAVIA,ITALY. UNIV VARESE,OSTET GINECOL CLIN,VARESE,ITALY. CNR,CTR STUDIO ISTOCHIM,PAVIA,ITALY.
authors: G
authors: G
authors: Franchini
authors: A
authors: A
authors: Tusei
authors: L
authors: L
authors: Babilonti
authors: N
authors: N
authors: Donadello
authors: M
authors: M
authors: Franchi
authors: F
authors: F
authors: Zanaboni
authors: S
authors: S
authors: Tateo
authors: G
authors: G
authors: Mazzini
authors: A
authors: A
authors: Riccardi
authors: M
authors: M
authors: Danova