title: Vascular protective role of vitexicarpin isolated from Vitex rotundifolia in human umbilical vein endothelial cells.
abstract: Pro-inflammatory cytokines induce injury of endothelial cells caused by increases of adhesion molecules, leading to vascular inflammation and the development of atherosclerosis. Recent pharmacological studies have demonstrated that vitexicarpin, a flavonoid isolated from Vitex rotundifolia, has anti-inflammatory, antitumor, and analgesic properties. In this study, we investigated whether vitexicarpin (5-100 nM) prevented the TNF-α-induced vascular inflammation process in human umbilical vein endothelial cells (HUVEC). We found that pretreatment with vitexicarpin decreased TNF-α (10 ng/ml)-induced expression of cell adhesion molecules such as vascular cell adhesion molecule-1, intracellular adhesion molecule-1, and E-selectin as well as matrix metalloproteinase-2 and -9 expression. Preincubation with vitexicarpin also dose-dependently inhibited TNF-α-induced adhesion of HL-60 monocytic cells. Vitexicarpin significantly decreased TNF-α-induced intracellular reactive oxygen species (ROS) production. Furthermore, vitexicarpin suppressed NF-κB nuclear translocation and transcriptional activity in TNF-α-treated HUVEC. In conclusion, vitexicarpin significantly reduced vascular inflammation, through inhibition of ROS-NF-κB pathway in vascular endothelial cells.
authors: College of Oriental Medicine and Professional Graduate School of Oriental Medicine, Wonkwang University, Iksan, Chonbuk 570-749, Republic of Korea.
authors: So Min
authors: SM
authors: Lee
authors: Yun Jung
authors: YJ
authors: Lee
authors: Youn Chul
authors: YC
authors: Kim
authors: Jin Sook
authors: JS
authors: Kim
authors: Dae Gill
authors: DG
authors: Kang
authors: Ho Sub
authors: HS
authors: Lee
references: Blood. 1991 May 15;77(10):2266-71
references: 1709380
references: Arterioscler Thromb Vasc Biol. 2003 Sep 1;23(9):1559-66
references: 12893683
references: Anal Biochem. 1997 Nov 15;253(2):162-8
references: 9367498
references: Circ Res. 2007 Aug 3;101(3):234-47
references: 17673684
references: Cell Mol Immunol. 2004 Oct;1(5):343-50
references: 16285893
references: N Engl J Med. 1999 Jan 14;340(2):115-26
references: 9887164
references: Arterioscler Thromb Vasc Biol. 2005 May;25(5):904-14
references: 15731497
references: J Biol Chem. 2006 Jun 2;281(22):15099-109
references: 16554298
references: Planta Med. 1998 Aug;64(6):546-50
references: 9741302
references: Eur J Pharmacol. 2003 Sep 23;477(3):269-76
references: 14522366
references: Circulation. 1998 Nov 24;98(21):2255-61
references: 9826311
references: Food Chem Toxicol. 2000 Oct;38(10):861-5
references: 11039319
references: Biochem Biophys Res Commun. 2004 Jan 30;314(1):118-22
references: 14715254
references: Phytother Res. 2009 Nov;23(11):1516-20
references: 19441009
references: Am J Physiol. 1999 Nov;277(5):F711-22
references: 10564234
references: J Nat Med. 2009 Oct;63(4):463-7
references: 19672675
references: J Biol Chem. 2000 Dec 29;275(52):41415-23
references: 10998420
references: Curr Pharm Des. 2009;15(26):2988-3002
references: 19754375
references: Nature. 2002 Dec 19-26;420(6917):868-74
references: 12490960
references: Circulation. 2004 Nov 30;110(22):3480-7
references: 15545515
references: Phytother Res. 2001 Sep;15(6):535-7
references: 11536386
references: J Clin Invest. 2003 Oct;112(8):1176-85
references: 14561702
references: Phytother Res. 2002 May;16(3):295-8
references: 12164283
references: Clin Investig. 1993 Jan;71(1):3-6
references: 8453256
references: J Clin Oncol. 2009 Aug 10;27(23):3808-14
references: 19470922
references: Intensive Care Med. 2001 Apr;27(4):751-6
references: 11398704