title: Valine 1532 of human BRC repeat 4 plays an important role in the interaction between BRCA2 and RAD51.
abstract: The breast cancer susceptibility protein BRCA2 is essential for recombinational DNA repair. BRCA2 specifically binds to RAD51 via eight BRC repeat motifs and delivers RAD51 to double-stranded DNA breaks. In this study, a mammalian two-hybrid assay and competitive ELISA showed that the interaction between BRC repeat 4 (BRC4) and RAD51 was strengthened by the substitution of a single BRC4 amino acid from valine to isoleucine (V1532I). However, the cancer-associated V1532F mutant exhibited very weak interaction with RAD51. This study used a comparative analysis of BRC4 between animal species to identify V1532 as an important residue that interacts with RAD51.
authors: Department of Basic Science, School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, Tokyo, Japan.
authors: Kazuhiko
authors: K
authors: Ochiai
authors: Yasunaga
authors: Y
authors: Yoshikawa
authors: Kumiko
authors: K
authors: Yoshimatsu
authors: Toshina
authors: T
authors: Oonuma
authors: Yukiko
authors: Y
authors: Tomioka
authors: Eichi
authors: E
authors: Takeda
authors: Jiro
authors: J
authors: Arikawa
authors: Katsumi
authors: K
authors: Mominoki
authors: Toshinori
authors: T
authors: Omi
authors: Kazuyoshi
authors: K
authors: Hashizume
authors: Masami
authors: M
authors: Morimatsu