title: Olfactory trace conditioning in Drosophila.
abstract: The neural representation of a sensory stimulus evolves with time, and animals keep that representation even after stimulus cessation (i.e., a stimulus "trace"). To contrast the memories of an odor and an odor trace, we here establish a rigorous trace conditioning paradigm in the fruit fly, Drosophila melanogaster. We modify the olfactory associative learning paradigm, in which the odor and electric shock are presented with a temporal overlap (delay conditioning). Given a few-second temporal gap between the presentations of the odor and the shock in trace conditioning, the odor trace must be kept until the arrival of electric shock to form associative memory. We found that memories after trace and delay conditioning have striking similarities: both reached the same asymptotic learning level, although at different rates, and both kinds of memory have similar decay kinetics and highly correlated generalization profiles across odors. In search of the physiological correlate of the odor trace, we used in vivo calcium imaging to characterize the odor-evoked activity of the olfactory receptor neurons in the antennal lobe. After the offset of odor presentation, the receptor neurons showed persistent, odor-specific response patterns that lasted for a few seconds and were fundamentally different from the response patterns during the stimulation. Weak correlation between the behavioral odor generalization profile in trace conditioning and the physiological odor similarity profiles in the antennal lobe suggest that the odor trace used for associative learning may be encoded downstream of the olfactory receptor neurons.
authors: Max-Planck Institut für Neurobiologie, 82152 Martinsried, Germany.
authors: Dana Shani
authors: DS
authors: Galili
authors: Alja
authors: A
authors: Lüdke
authors: C Giovanni
authors: CG
authors: Galizia
authors: Paul
authors: P
authors: Szyszka
authors: Hiromu
authors: H
authors: Tanimoto
references: Neural Comput. 2006 Jan;18(1):10-25
references: 16354378
references: Curr Biol. 2009 Aug 25;19(16):R700-13
references: 19706282
references: Cell. 2004 Jun 25;117(7):965-79
references: 15210116
references: Curr Opin Neurobiol. 2004 Dec;14(6):737-44
references: 15582377
references: Proc Natl Acad Sci U S A. 2004 Jul 13;101(28):10476-81
references: 15232007
references: Neuron. 2004 Sep 2;43(5):703-14
references: 15339651
references: Annu Rev Neurosci. 2005;28:275-302
references: 16022597
references: Neuron. 2007 Apr 5;54(1):89-103
references: 17408580
references: J Exp Biol. 1997 Feb;200(Pt 4):837-47
references: 9076967
references: J Neurosci. 2011 May 18;31(20):7229-39
references: 21593307
references: J Neurosci. 2004 Jul 21;24(29):6507-14
references: 15269261
references: Front Syst Neurosci. 2008 Jun 24;2:3
references: 18958247
references: Proc Biol Sci. 2009 Oct 7;276(1672):3413-20
references: 19586944
references: Neuron. 2008 Feb 28;57(4):586-98
references: 18304487
references: J Neurosci. 2007 Oct 31;27(44):11966-77
references: 17978037
references: Science. 2004 Jan 16;303(5656):366-70
references: 14684826
references: J Neurosci. 2007 Feb 14;27(7):1659-69
references: 17301174
references: Behav Neurosci. 2009 Oct;123(5):1095-101
references: 19824776
references: Science. 1998 Apr 3;280(5360):77-81
references: 9525860
references: Trends Neurosci. 2007 Mar;30(3):92-100
references: 17224191
references: Neuron. 2010 Feb 25;65(4):516-29
references: 20188656
references: Behav Neurosci. 1986 Oct;100(5):729-44
references: 3778636
references: Cell. 2003 Jan 24;112(2):271-82
references: 12553914
references: Neuron. 2004 May 13;42(3):437-49
references: 15134640
references: Curr Biol. 2005 Nov 8;15(21):1953-60
references: 16271874
references: Nature. 2004 Aug 26;430(7003):983
references: 15329711
references: Annu Rev Neurosci. 1994;17:519-49
references: 8210186
references: Science. 2002 Jul 19;297(5580):359-65
references: 12130775
references: Nature. 2008 Jun 26;453(7199):1244-7
references: 18509336
references: Neuron. 2002 Aug 29;35(5):951-60
references: 12372288
references: Neuron. 2009 Nov 25;64(4):510-21
references: 19945393
references: Neuron. 2005 Nov 23;48(4):661-73
references: 16301181
references: J Comp Physiol A. 1985 Sep;157(2):263-77
references: 3939242
references: Neuron. 2008 Jul 31;59(2):311-21
references: 18667158
references: Neurobiol Learn Mem. 2001 Nov;76(3):447-61
references: 11726247
references: Nat Biotechnol. 2001 Feb;19(2):137-41
references: 11175727
references: Annu Rev Psychol. 2006;57:87-115
references: 16318590
references: Behav Neurosci. 1996 Jun;110(3):427-35
references: 8888987
references: Learn Mem. 2007 Jul 12;14(7):491-6
references: 17626907
references: Hippocampus. 1998;8(6):620-6
references: 9882019
references: J Neurosci. 2008 Dec 3;28(49):13075-87
references: 19052198
references: Proc Natl Acad Sci U S A. 2008 Oct 14;105(41):15985-90
references: 18824685
references: Learn Mem. 2005 Mar-Apr;12(2):86-95
references: 15805307
references: Behav Brain Res. 1999 Mar;99(2):123-32
references: 10512579
references: Hippocampus. 1998;8(6):638-46
references: 9882021
references: Trends Cogn Sci. 2002 Dec 1;6(12):524-531
references: 12475713
references: Trends Neurosci. 2008 Feb;31(2):105-12
references: 18199490
references: Nat Neurosci. 2007 Nov;10(11):1474-82
references: 17922008
references: Proc Natl Acad Sci U S A. 2003 Feb 4;100(3):1399-404
references: 12552137
references: Front Behav Neurosci. 2010 Jun 02;4:27
references: 20577637
references: J Neurogenet. 2010 Sep;24(3):168-75
references: 20807100
references: Nat Neurosci. 2008 Oct;11(10):1177-84
references: 18794840
references: J Neurophysiol. 2005 Nov;94(5):3303-13
references: 16014792
references: PLoS One. 2008 Jul 30;3(7):e2810
references: 18665249
references: J Neurophysiol. 2008 Feb;99(2):734-46
references: 18094099