title: Melanoma-specific cytotoxicity of a human MGSA/GRO alpha C-terminal peptide conjugated to daunorubicin.
abstract: We report the conjugation of the C-terminal alpha-helix of human melanoma growth stimulating activity to daunorubicin using the novel linker polyethylene glycol (3400) dialdehyde. The technique allows us to control the drug:peptide ratio in the conjugate. We also describe the use of capillary zone electrophoresis and HPLC to monitor both the reaction and the stability of the peptide to various pH conditions. We found that the conjugate is about 10-fold more active on human Hs294t and murine B16 melanoma eel lines (which express the MGSA receptor) than the free drug while the same conjugate has little activity on human SKOV-3 and CCL-121 non-melanoma cell lines.
authors: UNIV LAVAL,FAC MED,DEPT BIOCHEM,QUEBEC CITY,PQ G1K 7P4,CANADA.
authors: P
authors: P
authors: Roby
authors: M
authors: M
authors: Page