title: Using the Flp-In™ T-Rex™ system to regulate GPCR expression.
abstract: The development of a cell-based system that allows the integration of a gene of interest (GOI), such as a G protein-coupled receptor (GPCR), into a specific site on the genome, has made the generation of mammalian cell lines able to express such proteins easy and efficient. Flp-In™ stable cell lines are isogenic and hence protein expression is constant across a population of cells. A useful addition to the Flp-In™ system (Flp-In™ T-Rex™) allows this expression to be controlled by the addition of a small molecule inducer to the cell culture medium. Stable cell lines generated as described here can be used to great advantage in the study of receptor pharmacology signalling and oligomerisation.
authors: Neuroscience and Molecular Pharmacology, Biomedical & Life Sciences, University of Glasgow, Glasgow, UK.
authors: Richard J
authors: RJ
authors: Ward
authors: Elisa
authors: E
authors: Alvarez-Curto
authors: Graeme
authors: G
authors: Milligan