title: Characterization of the duck enteritis virus UL55 protein.
abstract: BACKGROUND
  Characteration of the newly identified duck enteritis virus UL55 gene product has not been reported yet. Knowledge of the protein UL55 can provide useful insights about its function.
  
  
  RESULTS
  The newly identified duck enteritis virus UL55 gene was about 561 bp, it was amplified and digested for construction of a recombinant plasmid pET32a(+)/UL55 for expression in Escherichia coli. SDS-PAGE analysis revealed the recombinant protein UL55(pUL55) was overexpressed in Escherichia coli BL21 host cells after induction by 0.2 mM IPTG at 37°C for 4 h and aggregated as inclusion bodies. The denatured protein about 40 KDa named pUL55 was purified by washing five times, and used to immune rabbits for preparation of polyclonal antibody. The prepared polyclonal antibody against pUL55 was detected and determined by Agar immundiffusion and Neutralization test. The results of Wstern blotting assay and intracellular analysis revealed that pUL55 was expressed most abundantly during the late phase of replication and mainly distributed in cytoplasm in duck enteritis virus infected cells.
  
  
  CONCLUSIONS
  In this study, the duck enteritis virus UL55 protein was successfully expressed in prokaryotic expression system. Besides, we have prepared the polyclonal antibody against recombinant prtein UL55, and characterized some properties of the duck enteritis virus UL55 protein for the first time. The research will be useful for further functional analysis of this gene.
authors: Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu city, Sichuan, 611130, PR China.
authors: Ying
authors: Y
authors: Wu
authors: Anchun
authors: A
authors: Cheng
authors: Mingshu
authors: M
authors: Wang
authors: Shunchuan
authors: S
authors: Zhang
authors: Dekang
authors: D
authors: Zhu
authors: Renyong
authors: R
authors: Jia
authors: Qihui
authors: Q
authors: Luo
authors: Zhengli
authors: Z
authors: Chen
authors: Xiaoyue
authors: X
authors: Chen
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