title: Proteomic analysis of effects on natural herb additive containing immunoglobulin Yolksac (IgY) in pigs.
abstract: Thirty male pigs were infected orally with E. coli and Salmonella typhimurium, and divided into a control group and two additive groups to determine the effect of an additive mixture on the changes in protein expression. The pigs were given a food supplemented with a natural herbal additive containing immunoglobulin yolksac (IgY) at concentrations of 0.5% or 1%. On the 1st day and after eight weeks of feeding, the body weight gain, food intake and serum GOT/GPT levels were examined. The GOT/GPT levels on the 1st day were similar in the three groups. However, after eight weeks of feeding, the GOT level was significantly lower in the additive treatment groups (0.5% and 1.0%). In addition, the changes in the spleen proteome as a response to the herbal additive were examined using two-dimensional polyacrylamide gel electrophoresis. A total of 31 differentially expressed protein spots were identified by comparing the protein profiles of the control and additive treated porcine spleens. Finally, 19 proteins were detected by MALDI-TOF/MS. Overall, the proteins detected are involved in a range of biological process, such as metabolic processes, biological processes, transport, carbohydrate metabolic processes, generation of precursors and energy. In conclusion, these results support of the hypothesis that a natural herbal additive containing IgY can affect the immune regulation system and reduce the stress of microbial infections.
authors: Research Institute of Life Science and College of Veterinary Medicine, Gyeongsang National University, Republic of Korea.
authors: Hyeon-Soo
authors: HS
authors: Park
authors: Kwang-Il
authors: KI
authors: Park
authors: Arulkumar
authors: A
authors: Nagappan
authors: Do-Hoon
authors: DH
authors: Lee
authors: Sang-Rim
authors: SR
authors: Kang
authors: Jin-A
authors: JA
authors: Kim
authors: Eun Hee
authors: EH
authors: Kim
authors: Dae-Yong
authors: DY
authors: Han
authors: Gon-Sup
authors: GS
authors: Kim