title: [Immunogenic and protective properties of nanosized constructs based on tubular immunostimulating complexes and pore forming protein of Yersinia pseudotuberculosis].
abstract: OBJECTIVE
  Evaluation of immunogenic and protective properties of constructs based on subunit porin antigen from Yersinia pseudotuberculosis, immunostimulating complexes (ISCOM) and tubular immunostimulating (TI) complexes.
  
  
  METHODS
  Porin antibodies and blood serum cytokines were determined by using EIA. Porin-specific cell immunity was evaluated by DTH reaction inflammation index. Protective activity of porin formulations was determined by measuring specific gravity of animals surviving Yersinia pseudotuberculosis lethal challenge.
  
  
  RESULTS
  Porin in TI complexes develops higher immunogenicity when compared with individual protein or protein with complete Freunds adjuvant. Porin in TI complexes develops higher protective activity, inhibits interferon synthesis in mice. Incorporation of porin into TI complexes results in neutralization of porin suppressive activity against DTH mechanisms and interferon system.
  
  
  CONCLUSIONS
  TI complexes may be used as perspective carriers for bacterial antigens. TI complexes have adjuvant properties and can provide protective properties to porin vaccine constructs.
authors: A V
authors: AV
authors: Tsybul'skiĭ
authors: A M
authors: AM
authors: Popov
authors: N M
authors: NM
authors: Sanina
authors: A N
authors: AN
authors: Mazeĭka
authors: O Iu
authors: OIu
authors: Portniagina
authors: O D
authors: OD
authors: Novikova
authors: N F
authors: NF
authors: Timchenko
authors: É Ia
authors: ÉIa
authors: Kostetskiĭ