title: Phosphate utilization efficiency correlates with expression of low-affinity phosphate transporters and noncoding RNA, IPS1, in barley.
abstract: Genetic variation in phosphorus (P) efficiency exists among wheat (Triticum aestivum) and barley (Hordeum vulgare) genotypes, but the underlying mechanisms for the variation remain elusive. High- and low-affinity phosphate (Pi) PHT1 transporters play an indispensable role in P acquisition and remobilization. However, little is known about genetic variation in PHT1 gene expression and association with P acquisition efficiency (PAE) and P utilization efficiency (PUE). Here, we present quantitative analyses of transcript levels of high- and low-affinity PHT1 Pi transporters in four barley genotypes differing in PAE. The results showed that there was no clear pattern in the expression of four paralogs of the high-affinity Pi transporter HvPHT1;1 among the four barley genotypes, but the expression of a low-affinity Pi transporter, HvPHT1;6, and its close homolog HvHPT1;3 was correlated with the genotypes differing in PUE. Interestingly, the expression of HvPHT1;6 and HvPHT1;3 was correlated with the expression of HvIPS1 (for P starvation inducible; noncoding RNA) but not with HvIPS2, suggesting that HvIPS1 plays a distinct role in the regulation of the low-affinity Pi transporters. In addition, high PUE was found to be associated with high root-shoot ratios in low-P conditions, indicating that high carbohydrate partitioning into roots occurs simultaneously with high PUE. However, high PUE accompanying high carbon partitioning into roots could result in low PAE. Therefore, the optimization of PUE through the modification of low-affinity Pi transporter expression may assist further improvement of PAE for low-input agriculture systems.
authors: Australian Centre for Plant Functional Genomics, University of Adelaide, Glen Osmond, South Australia 5064, Australia. chunyuan.huang@adelaide.edu.au
authors: Chun Y
authors: CY
authors: Huang
authors: Neil
authors: N
authors: Shirley
authors: Yusuf
authors: Y
authors: Genc
authors: Bujun
authors: B
authors: Shi
authors: Peter
authors: P
authors: Langridge
references: Planta. 2005 Nov;222(4):688-98
references: 16133217
references: RNA. 2004 Oct;10(10):1507-17
references: 15383676
references: Plant Mol Biol. 2003 Sep;53(1-2):27-36
references: 14756304
references: Proc Nutr Soc. 2010 Nov;69(4):592-600
references: 20860858
references: Plant Cell Environ. 2011 Apr;34(4):681-9
references: 21309796
references: J Exp Bot. 2004 Apr;55(398):855-65
references: 15020637
references: Plant Cell. 2004 Aug;16(8):2001-19
references: 15258262
references: BMC Genomics. 2011 Feb 25;12:129
references: 21352554
references: Plant J. 2004 Aug;39(4):629-42
references: 15272879
references: Trends Plant Sci. 2006 Dec;11(12):610-7
references: 17092760
references: Plant Physiol. 2008 Jun;147(2):732-46
references: 18390805
references: Plant Physiol. 2006 Jul;141(3):988-99
references: 16679424
references: Planta. 1984 May;160(6):500-7
references: 24258776
references: Plant Physiol. 2008 Apr;146(4):1673-86
references: 18263782
references: J Exp Bot. 2009;60(5):1427-38
references: 19168668
references: J Genet Genomics. 2009 Aug;36(8):455-66
references: 19683668
references: Genome Biol. 2002 Jun 18;3(7):RESEARCH0034
references: 12184808
references: Funct Integr Genomics. 2006 Jul;6(3):202-11
references: 16547597
references: Plant Physiol. 2006 Jul;141(3):1000-11
references: 16679417
references: Plant Physiol. 2008 Apr;146(4):1821-33
references: 18258691
references: Curr Opin Plant Biol. 2008 Oct;11(5):536-40
references: 18614391
references: Ann Bot. 2006 Oct;98(4):693-713
references: 16769731
references: Annu Rev Plant Biol. 2006;57:19-53
references: 16669754
references: Curr Biol. 2005 Nov 22;15(22):2038-43
references: 16303564
references: Plant Cell. 2006 Feb;18(2):412-21
references: 16387831
references: Plant J. 2010 May;62(3):508-17
references: 20149131
references: Proc Natl Acad Sci U S A. 2002 Oct 1;99(20):13324-9
references: 12271140
references: Plant Physiol. 2010 Mar;152(3):1431-41
references: 20053709
references: J Exp Bot. 2008;59(1):93-109
references: 18212031
references: Nat Genet. 2007 Aug;39(8):1033-7
references: 17643101
references: Plant J. 2009 Mar;57(5):798-809
references: 18980647
references: Plant Cell Physiol. 2010 Dec;51(12):2119-31
references: 21062869
references: Cell. 2005 Apr 22;121(2):207-21
references: 15851028
references: J Exp Bot. 2007;58(11):2775-84
references: 17609531
references: Plant Mol Biol. 2007 Nov;65(4):547-70
references: 17703278
references: Plant J. 2006 Mar;45(5):712-26
references: 16460506
references: Plant J. 2002 Aug;31(3):341-53
references: 12164813
references: Annu Rev Plant Physiol Plant Mol Biol. 1999 Jun;50:665-693
references: 15012223
references: Plant J. 2000 Dec;24(5):559-67
references: 11123795
references: Science. 2010 Feb 12;327(5967):812-8
references: 20110467
references: Ann Bot. 2010 Apr;105(4):513-26
references: 20181569
references: J Exp Bot. 2004 Feb;55(396):285-93
references: 14718495
references: Plant Mol Biol. 1997 Mar;33(5):867-74
references: 9106510
references: Trends Plant Sci. 2008 Jul;13(7):359-67
references: 18501664
references: Plant J. 2009 Mar;57(5):895-904
references: 19000161
references: New Phytol. 2003 Mar;157(3):423-447
references: 33873400
references: Plant Physiol. 2000 Dec;124(4):1786-99
references: 11115894
references: Plant Cell Physiol. 2008 May;49(5):691-703
references: 18344526
references: J Exp Bot. 2009;60(7):1953-68
references: 19346243