title: An early inflammatory gene profile in visceral adipose tissue in children.
abstract: The aim of this study was to characterize expression profiles of visceral and subcutaneous adipose tissue in children. Adipose tissue samples were collected from children having elective surgery (n = 71, [54 boys], 6.0 ± 4.3 years). Affymetrix microarrays (n = 20) were performed to characterize the functional profile and identify genes of interest in adipose tissue. Visceral adipose tissue had an overrepresentation of Gene Ontology themes related to immune and inflammatory responses and subcutaneous adipose tissue had an overrepresentation of themes related to adipocyte growth and development. Likewise, qPCR performed in the whole cohort showed a 30-fold increase in haptoglobin (P = 0.005), 7-fold increase in IL-10 (P < 0.001), 8-fold decrease in VEGF (P = 0.01) and a 28-fold decrease in TBOX15 (P < 0.001) in visceral compared to subcutaneous adipose tissue. The inflammatory pattern in visceral adipose tissue may represent an early stage of the adverse effects of this depot, and combined with chronic obesity, may contribute to increased metabolic and cardiovascular risk.
authors: Institute of Endocrinology and Diabetes, The Children's Hospital, University of Sydney , Westmead , Australia . tam@pbrc.edu
authors: Charmaine S
authors: CS
authors: Tam
authors: Leonie K
authors: LK
authors: Heilbronn
authors: Corneliu
authors: C
authors: Henegar
authors: Melanie
authors: M
authors: Wong
authors: Christopher T
authors: CT
authors: Cowell
authors: Mark J
authors: MJ
authors: Cowley
authors: Warren
authors: W
authors: Kaplan
authors: Karine
authors: K
authors: Clément
authors: Louise A
authors: LA
authors: Baur
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