title: Development of seven novel EST-SSR markers from Cycas panzhihuaensis (Cycadaceae).
abstract: OBJECTIVE
  Cycas panzhihuaensis L. Zhou & S. Y. Yang is a vulnerable gymnosperm endemic to China, where its range represents the northernmost occupation of Cycas L. We developed EST-derived SSR markers to investigate its genetic diversity and population structure. •
  
  
  RESULTS
  Based on the expressed sequence tag (EST) database for Cycas rumphii Miq., seven simple sequence repeat (SSR) markers were identified and screened in 55 individuals from seven wild populations of C. panzhihuaensis. Alleles numbered 2 to 5, and their observed heterozygosity and expected heterozygosity ranged from 0.0000 to 0.6545 and from 0.0535 to 0.6966, respectively. •
  
  
  CONCLUSIONS
  These new EST-SSR markers will enhance further studies of the population genetics of C. panzhihuaensis, allowing researchers to design reasonable conservation and management protocols.
authors: Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, P. R. China.
authors: Fangming
authors: F
authors: Zhang
authors: Tao
authors: T
authors: Su
authors: Ying
authors: Y
authors: Yang
authors: Yanhong
authors: Y
authors: Zhai
authors: Yunheng
authors: Y
authors: Ji
authors: Shaotian
authors: S
authors: Chen