title: Phosphorylation by MPK6: a conserved transcriptional modification mediates nitrate reductase activation and NO production?
abstract: Nitrate reductase is a central enzyme of nitrogen assimilation in plants. In a recent work, we have revealed MPK6 could phosphorylate Arabidopsis NIA2 at the serine 627 in hinge 2 region, this phosporylation may represent a rapid activation mechnism when plant need excessive nitrate reduction. Interestingly, all eukaryotic NRs have conserved docking sequence in their FAD domains, and many plant NR proteins have the conserved MAPK phosphorylation site. Those results indicated the MAPK cascades, the conserved signaling pathway also involved in lateral root development, mediated of NR phosporylation and NO generation. We noticed that the phosphorylation of S627 residue by MPK6 have a specially influence on the NO generation activity of NIA2. Although no homology of mammalian NOS has been identified in plants, NR may still share a similar regulation mechanism with mammalian NOS.
authors: Laboratory of Plant Stress Biology, Department of Biology, Henan University, Kaifeng, China.
authors: Pengcheng
authors: P
authors: Wang
authors: Yanyan
authors: Y
authors: Du
authors: Chun-Peng
authors: CP
authors: Song
references: Plant Cell. 1996 Mar;8(3):519-27
references: 8721753
references: Plant Cell. 2007 Mar;19(3):805-18
references: 17369371
references: Nature. 1999 Jun 10;399(6736):601-5
references: 10376603
references: Plant Cell. 2004 Dec;16(12):3386-99
references: 15539472
references: Plant Physiol. 1988 Oct;88(2):389-95
references: 16666314
references: Mol Plant. 2008 Jul;1(4):645-58
references: 19825569
references: J Exp Bot. 2006;57(3):471-8
references: 16356941
references: Plant Cell. 1996 Mar;8(3):505-17
references: 8721752
references: Plant J. 2006 Jan;45(1):113-22
references: 16367958
references: Nature. 2002 Feb 28;415(6875):977-83
references: 11875555
references: Plant J. 2003 Sep;35(5):566-73
references: 12940950
references: Nature. 2008 Feb 14;451(7180):789-95
references: 18273012
references: Plant Cell. 2008 May;20(5):1390-406
references: 18515503
references: Plant Cell. 2000 Oct;12(10):1849-62
references: 11041881
references: Plant J. 2010 Jan;61(2):234-48
references: 19832943
references: Plant Physiol. 2005 Jun;138(2):1097-105
references: 15908593
references: J Mol Evol. 1996 Apr;42(4):432-42
references: 8642612
references: Trends Plant Sci. 2009 May;14(5):270-9
references: 19375376
references: Plant Physiol. 2009 May;150(1):167-77
references: 19251906
references: Proc Natl Acad Sci U S A. 2002 Dec 10;99(25):16314-8
references: 12446847
references: Chembiochem. 2002 Mar 1;3(2-3):198-206
references: 11921398
references: Plant Cell. 2007 Jan;19(1):63-73
references: 17259259
references: Plant Cell. 2008 Mar;20(3):602-13
references: 18364464
references: Annu Rev Plant Physiol Plant Mol Biol. 1999 Jun;50:277-303
references: 15012211
references: Mol Cell. 2004 Jul 2;15(1):141-52
references: 15225555
references: J Plant Physiol. 2008 Jun 16;165(9):967-75
references: 17936409
references: Plant Cell Physiol. 2006 Jun;47(6):726-35
references: 16608868
references: Nature. 1999 Jun 10;399(6736):597-601
references: 10376602
references: Plant Cell. 2010 Sep;22(9):2981-98
references: 20870959
references: Plant Cell. 2003 Oct;15(10):2320-32
references: 14508004
references: Proc Natl Acad Sci U S A. 2008 Apr 8;105(14):5638-43
references: 18378893