title: Genome-wide RNA-seq analysis of human and mouse platelet transcriptomes.
abstract: Inbred mice are a useful tool for studying the in vivo functions of platelets. Nonetheless, the mRNA signature of mouse platelets is not known. Here, we use paired-end next-generation RNA sequencing (RNA-seq) to characterize the polyadenylated transcriptomes of human and mouse platelets. We report that RNA-seq provides unprecedented resolution of mRNAs that are expressed across the entire human and mouse genomes. Transcript expression and abundance are often conserved between the 2 species. Several mRNAs, however, are differentially expressed in human and mouse platelets. Moreover, previously described functional disparities between mouse and human platelets are reflected in differences at the transcript level, including protease activated receptor-1, protease activated receptor-3, platelet activating factor receptor, and factor V. This suggests that RNA-seq is a useful tool for predicting differences in platelet function between mice and humans. Our next-generation sequencing analysis provides new insights into the human and murine platelet transcriptomes. The sequencing dataset will be useful in the design of mouse models of hemostasis and a catalyst for discovery of new functions of platelets. Access to the dataset is found in the "Introduction."
authors: Program in Molecular Medicine, University of Utah School of Medicine, Salt Lake City, UT, USA.
authors: Jesse W
authors: JW
authors: Rowley
authors: Andrew J
authors: AJ
authors: Oler
authors: Neal D
authors: ND
authors: Tolley
authors: Benjamin N
authors: BN
authors: Hunter
authors: Elizabeth N
authors: EN
authors: Low
authors: David A
authors: DA
authors: Nix
authors: Christian C
authors: CC
authors: Yost
authors: Guy A
authors: GA
authors: Zimmerman
authors: Andrew S
authors: AS
authors: Weyrich
references: J Thromb Haemost. 2005 Aug;3(8):1725-36
references: 16102039
references: Blood. 2010 Sep 16;116(11):1951-7
references: 20538795
references: Nature. 1998 Aug 13;394(6694):690-4
references: 9716134
references: Blood. 1996 Feb 15;87(4):1238-42
references: 8608210
references: Biochem Pharmacol. 1985 May 1;34(9):1491-5
references: 2986648
references: Genome Biol. 2003;4(5):P3
references: 12734009
references: Thromb Haemost. 2005 Aug;94(2):412-21
references: 16113833
references: PLoS Comput Biol. 2009 Dec;5(12):e1000598
references: 20011106
references: Circulation. 2010 Jul 13;122(2):119-29
references: 20606121
references: Thromb Haemost. 1999 Feb;81(2):177-88
references: 10063988
references: Science. 2008 Aug 15;321(5891):956-60
references: 18599741
references: Nature. 1996 Jun 6;381(6582):516-9
references: 8632823
references: Nucleic Acids Res. 2007 Jan;35(Database issue):D61-5
references: 17130148
references: Nat Protoc. 2009;4(1):44-57
references: 19131956
references: J Thromb Haemost. 2010 Feb;8(2):369-78
references: 19943878
references: Blood. 2003 Mar 15;101(6):2285-93
references: 12433680
references: Cell. 2005 Aug 12;122(3):379-91
references: 16096058
references: Bioinformatics. 2009 Oct 15;25(20):2730-1
references: 19654113
references: J Thromb Haemost. 2009 Nov;7(11):1759-66
references: 19691483
references: Cell. 2008 May 2;133(3):523-36
references: 18423832
references: J Immunol. 1999 Apr 1;162(7):4311-8
references: 10201963
references: Nature. 2008 Jun 26;453(7199):1239-43
references: 18488015
references: Nucleic Acids Res. 2004 Jan 1;32(Database issue):D493-6
references: 14681465
references: Science. 2008 Jun 6;320(5881):1344-9
references: 18451266
references: Nat Rev Genet. 2009 Jan;10(1):57-63
references: 19015660
references: BMC Bioinformatics. 2008 Dec 05;9:523
references: 19061503
references: Mol Cell Proteomics. 2006 Feb;5(2):226-33
references: 16263699
references: Blood. 2010 Nov 25;116(22):4646-56
references: 20833976
references: BMC Bioinformatics. 2010 Sep 09;11:455
references: 20828407
references: Thromb Haemost. 1996 Dec;76(6):860-6
references: 8972001
references: J Biol Chem. 2004 Aug 6;279(32):34032-7
references: 15163665
references: Nat Methods. 2008 Jul;5(7):621-8
references: 18516045
references: Blood. 1998 Nov 1;92(9):3035-41
references: 9787136
references: Circulation. 2007 Mar 27;115(12):1551-62
references: 17353439
references: J Thromb Haemost. 2007 Apr;5(4):661-9
references: 17403200
references: Blood. 2010 Jan 7;115(1):7-14
references: 19773543
references: Blood. 2010 Jan 7;115(1):2-3
references: 20056796
references: Blood. 2009 Jun 18;113(25):6419-27
references: 19221037
references: Nature. 2000 Apr 6;404(6778):609-13
references: 10766244
references: Blood. 2000 Aug 15;96(4):1399-408
references: 10942384
references: Blood. 1998 Jun 1;91(11):4152-7
references: 9596661
references: Blood. 2003 Oct 15;102(8):2851-5
references: 12816857
references: Nature. 1997 Apr 3;386(6624):502-6
references: 9087410
references: Lancet. 1975 Feb 8;1(7902):344-5
references: 46496
references: Circulation. 2006 May 16;113(19):2278-84
references: 16682612
references: Thromb Haemost. 2003 Oct;90(4):738-48
references: 14515197