title: Large scale library generation for high throughput sequencing.
abstract: BACKGROUND
  Large efforts have recently been made to automate the sample preparation protocols for massively parallel sequencing in order to match the increasing instrument throughput. Still, the size selection through agarose gel electrophoresis separation is a labor-intensive bottleneck of these protocols.
  
  
  RESULTS
  In this study a method for automatic library preparation and size selection on a liquid handling robot is presented. The method utilizes selective precipitation of certain sizes of DNA molecules on to paramagnetic beads for cleanup and selection after standard enzymatic reactions.
  
  
  CONCLUSIONS
  The method is used to generate libraries for de novo and re-sequencing on the Illumina HiSeq 2000 instrument with a throughput of 12 samples per instrument in approximately 4 hours. The resulting output data show quality scores and pass filter rates comparable to manually prepared samples. The sample size distribution can be adjusted for each application, and are suitable for all high throughput DNA processing protocols seeking to control size intervals.
authors: Science for Life Laboratory, Division of Gene Technology, Royal Institute of Technology (KTH), School of Biotechnology, Solna, Sweden.
authors: Erik
authors: E
authors: Borgström
authors: Sverker
authors: S
authors: Lundin
authors: Joakim
authors: J
authors: Lundeberg
references: Nat Biotechnol. 2009 Nov;27(11):1013-23
references: 19898456
references: Nature. 2010 Oct 28;467(7319):1061-73
references: 20981092
references: PLoS One. 2010 Apr 06;5(4):e10029
references: 20386591
references: PLoS Genet. 2010 Sep 09;6(9):e1001111
references: 20838461
references: J Natl Cancer Inst. 1973 Nov;51(5):1417-23
references: 4357758
references: Genome Biol. 2010;11(2):R15
references: 20137071
references: Genomics. 2009 Feb;93(2):105-11
references: 18992322
references: Nat Methods. 2009 Apr;6(4):291-5
references: 19287394
references: PLoS One. 2008;3(12):e3839
references: 19052635
references: J Biomed Biotechnol. 2009;2009:396808
references: 19841682
references: Int J Cancer. 1967 Sep 15;2(5):434-47
references: 6081590
references: Exp Cell Res. 2010 May 1;316(8):1339-43
references: 20211618
references: Nat Methods. 2008 Dec;5(12):1005-10
references: 19034268
references: Nat Genet. 2010 Nov;42(11):969-72
references: 20890277
references: J Biomed Biotechnol. 2010;2010:617469
references: 20625503
references: BMC Genomics. 2009 May 12;10:221
references: 19435513
references: Nat Rev Genet. 2010 Jan;11(1):31-46
references: 19997069