title: [Identification specific T lymphocyte epitopes on E6 protein of human papillomavirus type 18 in mice].
abstract: OBJECTIVE
  To identify specific T lymphocyte epitope on E6 protein of human papillomavirus type 18 in mice.
  
  
  METHODS
  Infection with one recombinant vaccinia virus rVVJ18 E7, E6 respectively in C57 BL/6 and BALB/c mice, specific cellular immune responses were detected by ELISPOT or intracellular cytokine stainings by using a series of overlapping synthetic peptides covering full length of the amino acid sequence of E6 and E7 proteins or various truncated peptides.
  
  
  RESULTS
  The rVVJ18 E7, E6 generated significant E6 specific T-cell immune responses in vaccinated mice. Mapping of the epitope of E6 revealed that the peptides E6(67-75 ( KCIDFYSRI) and E6(60-68) (IPHAAGHKC) presented respectively by C57BL/6 and BALB/c mice were the optimal peptides to activate E6-specific CD8+ T lymphocytes. However no positive cellular immune responses stimulated with various E7 peptides were detected by ELISPOT in immunized mice.
  
  
  CONCLUSIONS
  Two specific T lymphocyte epitopes were identified on E6 protein in C57BL/6 and BALB/c mice, which will provide the basis to evaluate cellular immune response elicited by HPV18 E6 protein based vaccine.
authors: Institute for Viral Disease Control and Prevention, China CDC, Beijing 100052, China.
authors: Li
authors: L
authors: Zhao
authors: Jiao
authors: J
authors: Ren
authors: Jing
authors: J
authors: Feng
authors: Zheng
authors: Z
authors: Pang
authors: Zhong-Xian
authors: ZX
authors: Zhang
authors: Wen-Jie
authors: WJ
authors: Tan
authors: Hou-Wen
authors: HW
authors: Tian
authors: Li
authors: L
authors: Ruan