title: Exacerbation of cigarette smoke-induced pulmonary inflammation by Staphylococcus aureus enterotoxin B in mice.
abstract: BACKGROUND
  Cigarette smoke (CS) is a major risk factor for the development of COPD. CS exposure is associated with an increased risk of bacterial colonization and respiratory tract infection, because of suppressed antibacterial activities of the immune system and delayed clearance of microbial agents from the lungs. Colonization with Staphylococcus aureus results in release of virulent enterotoxins, with superantigen activity which causes T cell activation.
  
  
  OBJECTIVE
  To study the effect of Staphylococcus aureus enterotoxin B (SEB) on CS-induced inflammation, in a mouse model of COPD.
  
  
  METHODS
  C57/Bl6 mice were exposed to CS or air for 4 weeks (5 cigarettes/exposure, 4x/day, 5 days/week). Endonasal SEB (10 μg/ml) or saline was concomitantly applied starting from week 3, on alternate days. 24 h after the last CS and SEB exposure, mice were sacrificed and bronchoalveolar lavage (BAL) fluid and lung tissue were collected.
  
  
  RESULTS
  Combined exposure to CS and SEB resulted in a raised number of lymphocytes and neutrophils in BAL, as well as increased numbers of CD8+ T lymphocytes and granulocytes in lung tissue, compared to sole CS or SEB exposure. Moreover, concomitant CS/SEB exposure induced both IL-13 mRNA expression in lungs and goblet cell hyperplasia in the airway wall. In addition, combined CS/SEB exposure stimulated the formation of dense, organized aggregates of B- and T- lymphocytes in lungs, as well as significant higher CXCL-13 (protein, mRNA) and CCL19 (mRNA) levels in lungs.
  
  
  CONCLUSIONS
  Combined CS and SEB exposure aggravates CS-induced inflammation in mice, suggesting that Staphylococcus aureus could influence the pathogenesis of COPD.
authors: Upper Airways Research Laboratory (URL), ENT Department, Ghent University Hospital, Ghent University, Ghent, Belgium.
authors: Wouter
authors: W
authors: Huvenne
authors: Ellen A
authors: EA
authors: Lanckacker
authors: Olga
authors: O
authors: Krysko
authors: Ken R
authors: KR
authors: Bracke
authors: Tine
authors: T
authors: Demoor
authors: Peter W
authors: PW
authors: Hellings
authors: Guy G
authors: GG
authors: Brusselle
authors: Guy F
authors: GF
authors: Joos
authors: Claus
authors: C
authors: Bachert
authors: Tania
authors: T
authors: Maes
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