title: [Study on antineoplastic effect of earthworm coelomic fluid in vitro].
abstract: OBJECTIVE
  To explore the antineoplastic effect in vitro of earthworm coelomic fluid (ECF)on growth inhibition and its mechanism for the tumor cell lines Siha, SW480, Colo205 and PC12.
  
  
  METHODS
  MTT colorimetric assay, flow cytometry and morphological analysis were used to test its antitumor activity on tumor cell lines and normal cell line Cos7 in vitro.
  
  
  RESULTS
  ECF can inhibit the cell growth of Siha, SW480, Colo205, PC12 and Cos7. But different tumor cell lines showed different sensitivity.
  
  
  CONCLUSIONS
  EFC can significantly inhibit the proliferation of tumor cells in vitro by inducing tumor cells apoptosis.
authors: Department of Life Sciences and Bioengineering, Beijing University of Technology, Beijing 100022, China.
authors: Jian-Hua
authors: JH
authors: Wang
authors: Tao
authors: T
authors: Guan
authors: Xiao-Yuan
authors: XY
authors: Wang
authors: Zhi-Zhen
authors: ZZ
authors: Liu
authors: Jian-Lin
authors: JL
authors: Zhang
authors: Xiu-Wei
authors: XW
authors: Wang
authors: Guo-Liang
authors: GL
authors: Wang
authors: Ru-Gang
authors: RG
authors: Zhong
authors: Bo
authors: B
authors: Niu