title: A study of the anti-inflammatory effect of the leaves of Psidium guajava Linn. on experimental animal models.
abstract: BACKGROUND
  The aim is to study the anti-inflammatory effect of the ethanolic extract of the leaves of Psidium guajava(PGE) on experimental animal models.
  
  
  METHODS
  Fresh leaves were collected, air-dried, powdered, and percolated in 95% ethanol. Acute toxicity test was done according to OECD guidelines. Four groups of animals of either sex, weighing 150-200g of the species Rattus norvegicus were taken for the study (n = 6). Group A was taken as control (3% gum acacia in 10 mL/kg body weight), Group B as test group (PGE 250 mg/kg body weight), Group C as test group (PGE 500 mg/kg body weight), and Group D as standard (Aspirin 100 mg/kg body weight). The animals were studied for acute inflammation by Carrageenan-induced rat paw edema, subacute inflammation by Granuloma pouch method, and chronic inflammation by Freund's adjuvant-induced arthritis method. Statistical analysis was done by one-way analysis of variance followed by multiple comparison tests.
  
  
  RESULTS
  In acute inflammation, there was significant inhibition of paw edema in Groups B, C, and D in comparison with Group A (P < 0.05). In subacute inflammation, there was significant inhibition of exudate formation in Groups B, C, and D in comparison to Group A (P < 0.05). In chronic inflammation, there was significant inhibition of paw edema and inhibition of weight reduction in Groups B, C, and D compared with Group A. Downregulation of arthritis index was also significant in Groups B, C, and D in comparison with Group A (P < 0.05).
  
  
  CONCLUSIONS
  The ethanolic extract of PGE has significant anti-inflammatory activity.
authors: Department of Pharmacology, Assam Medical College and Hospital, Dibrugarh - 786 002, Assam, India.
authors: Sarmistha
authors: S
authors: Dutta
authors: Swarnamoni
authors: S
authors: Das
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