title: Intact primary mitochondrial function in myotubes established from women with PCOS.
abstract: BACKGROUND
  Polycystic ovary syndrome (PCOS) affects 5-8% of fertile women and is often accompanied by insulin resistance, leading to increased risk of developing type 2 diabetes. Skeletal muscle from insulin-resistant PCOS subjects display reduced expression of nuclear encoded genes involved in mitochondrial oxidative metabolism.
  
  
  OBJECTIVE
  We aimed to investigate whether there was a primary mitochondrial dysfunction or difference in mitochondria content that might contribute to the in vivo detected insulin resistance.
  
  
  METHODS
  The ATP synthesis with and without ATP use and the mitochondrial mass was determined in mitochondria isolated from myotubes established from PCOS subjects and control subjects.
  
  
  METHODS
  Myotubes were established from eight insulin-resistant PCOS subjects (verified by euglycemic hyperinsulinemic clamp) and eight healthy weight- and age-matched controls.
  
  
  RESULTS
  Mitochondrial mass and measurable mitochondrial ATP synthesis, with and without ATP use, were not different between PCOS subjects and control subjects.
  
  
  CONCLUSIONS
  We found no evidence for a primary impaired mitochondrial function or content in myotubes established from PCOS subjects, and our results suggest that reduced expression of oxidative genes in PCOS subjects is an adaptive trait.
authors: Department of Endocrinology, Odense University Hospital, Odense, Denmark. mette.eriksen@ouh.regionsyddanmark.dk
authors: Mette Brandt
authors: MB
authors: Eriksen
authors: Ariane Denise
authors: AD
authors: Minet
authors: Dorte
authors: D
authors: Glintborg
authors: Michael
authors: M
authors: Gaster