title: Phylogenetic and similarity analysis of HTLV-1 isolates from HIV-coinfected patients from the south and southeast regions of Brazil.
abstract: HTLV-1 is endemic in Brazil and HIV/HTLV-1 coinfection has been detected, mostly in the northeast region. Cosmopolitan HTLV-1a is the main subtype that circulates in Brazil. This study characterized 17 HTLV-1 isolates from HIV coinfected patients of southern (n=7) and southeastern (n=10) Brazil. HTLV-1 provirus DNA was amplified by nested PCR (env and LTR) and sequenced. Env sequences (705 bp) from 15 isolates and LTR sequences (731 bp) from 17 isolates showed 99.5% and 98.8% similarity among sequences, respectively. Comparing these sequences with ATK (HTLV-1a) and Mel5 (HTLV-1c) prototypes, similarities of 99% and 97.4%, respectively, for env and LTR with ATK, and 91.6% and 90.3% with Mel5, were detected. Phylogenetic analysis showed that all sequences belonged to the transcontinental subgroup A of the Cosmopolitan subtype, clustering in two Latin American clusters.
authors: Laboratório de Retrovirus, Centro de Virologia, Instituto Adolfo Lutz, Secretaria de Estado da Saúde de São Paulo, Brazil.
authors: Mariana Cavalheiro
authors: MC
authors: Magri
authors: Luis Fernando de Macedo
authors: LF
authors: Brigido
authors: Rosangela
authors: R
authors: Rodrigues
authors: Helena Kaminami
authors: HK
authors: Morimoto
authors: João Leandro de Paula
authors: JL
authors: Ferreira
authors: Adele
authors: A
authors: Caterino-de-Araujo
references: AIDS Res Hum Retroviruses. 2006 Oct;22(10):953-9
references: 17067264
references: AIDS Res Hum Retroviruses. 2008 May;24(5):673-7
references: 18462080
references: J Acquir Immune Defic Syndr. 2001 Feb 1;26(2):185-90
references: 11242188
references: AIDS Res Hum Retroviruses. 2010 Dec;26(12):1333-9
references: 20929351
references: AIDS. 2006 Mar 21;20(5):780-2
references: 16514313
references: Mem Inst Oswaldo Cruz. 2005 Jul;100(4):371-6
references: 16113884
references: AIDS Res Hum Retroviruses. 1991 Nov;7(11):923-41
references: 1760230
references: J Biomed Sci. 2002 Nov-Dec;9(6 Pt 2):587-95
references: 12432224
references: J Med Virol. 2008 Nov;80(11):1966-71
references: 18814249
references: AIDS Res Hum Retroviruses. 2000 May 20;16(8):715-9
references: 10826478
references: Oncogene. 2005 Sep 5;24(39):6058-68
references: 16155612
references: AIDS Res Hum Retroviruses. 2005 Apr;21(4):256-62
references: 15943567
references: Virology. 1999 Aug 15;261(1):59-69
references: 10484750
references: J Med Virol. 2008 Nov;80(11):2040-5
references: 18814252