title: Binding of uranyl ion by a DNA aptamer attached to a solid support.
abstract: A UO(2)(2+)-specific DNA aptamer was attached to aminopolystyrene (aminoPS) using sulfo-SMCC as a crosslinking agent in view of high affinity of DNA for uranyl ion. Capacity of the aptamer-conjugated aminoPS resins for uranyl uptake was measured, revealing that about 0.63 μg of uranium can be complexed to 1g of the resins, which clearly demonstrates that most of DNA aptamers introduced to the resins can strongly bind to uranyl ion. In the presence of 21 mM bicarbonate ion at pH 8.01, apparent dissociation constant (K(d)(app)) of about 84.6 pM and log formation constant (K(f)) of about 22.9 were obtained. Results of the present study strongly suggest that modification of the aptamer-containing resins can improve uranyl-binding ability, probably leading to economical recovery of uranium from seawater.
authors: Department of Chemistry, Research Institute for Basic Sciences, and Research Center for New Nano Bio Fusion Technology, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul 130-701, Republic of Korea.
authors: Jisu
authors: J
authors: Kim
authors: Min Young
authors: MY
authors: Kim
authors: Hoon Sik
authors: HS
authors: Kim
authors: Sang Soo
authors: SS
authors: Hah