title: Cloning of the flap endonuclease-1 gene in Bombyx mori and identification of an antiapoptotic function.
abstract: The flap endonuclease-1 (FEN-1) gene is involved in DNA replication and repair, and it maintains genomic stability as well as the accuracy of DNA replication under normal growth conditions. However, FEN-1 also plays an important role in apoptosis and cancer development. We cloned the BmFEN-1 gene from Bombyx mori, which was 1343 bp in length and possessed an 1143 bp ORF (123-1266). It consists of seven introns and eight exons that encode a protein with 380 amino acids that has the typical XPG domain. The N-terminal motif is located at amino acids 95-105, and the proliferating cell nuclear antigen interaction motif is located at amino acids 337-344. RNA interference-mediated reduction of BmFEN-1 expression induced cell cycle arrest in S phase in BmE-SWU1 cells. These results suggest that BmFEN-1 can inhibit apoptosis and promote cell proliferation.
authors: The Key Sericultural Laboratory of Agricultural Ministry, Southwest University, Chongqing, PR China.
authors: Min-Hui
authors: MH
authors: Pan
authors: Juan
authors: J
authors: Du
authors: Jin-Ye
authors: JY
authors: Zhang
authors: Mao-Hua
authors: MH
authors: Huang
authors: Ting
authors: T
authors: Li
authors: Hong-Juan
authors: HJ
authors: Cui
authors: Cheng
authors: C
authors: Lu