title: Automated phase retrieval of a single-material object using a single out-of-focus image.
abstract: Phase retrieval is widely used in phase contrast microscopy. Here we present an autofocus algorithm that allows the phase of the exit wave function, from a single-material object, to be reconstructed at medium resolution from a single phase contrast image without any a priori knowledge of the imaging system or object. The algorithm is demonstrated on coherent out-of-focus electron micrographs of 30 nm latex sphere calibration standards, giving <10% RMS error over a large defocus range.
authors: School of Physics, Monash University, Victoria 3800, Australia. samuel.eastwood@monash.edu
authors: Samuel A
authors: SA
authors: Eastwood
authors: David M
authors: DM
authors: Paganin
authors: Amelia C Y
authors: AC
authors: Liu