title: Novel germ-cell tumor-cell line established from a testicular feminization syndrome.
abstract: We analyzed a novel cell line established from a testicular feminization syndrome. The original tumor was an endodermal sinus tumor with seminoma. The doubling time, the saturation density and the mitotic index were 36.1 hours, 3.65x10(4) cells/cm2 and 4.3%, respectively. The mode number of chromosomes was 102. Results of the MTT assay showed that the cell line had an IC50/PPC of less than 0.1 for methotrexate and actinomycin D. The effects of methotrexate were schedule dependent; the effects of actinomycin D were both schedule and dose dependent.
authors: KURUME UNIV,SCH MED,DEPT PATHOL 2,KURUME,FUKUOKA 830,JAPAN.
authors: K
authors: K
authors: Imamura
authors: Y
authors: Y
authors: Kiyozuka
authors: H
authors: H
authors: Nishimura
authors: M
authors: M
authors: Yakushiji