title: Chemoenzymatic synthesis of C8-modified sialic acids and related α2-3- and α2-6-linked sialosides.
abstract: Naturally occurring 8-O-methylated sialic acids, including 8-O-methyl-N-acetylneuraminic acid and 8-O-methyl-N-glycolylneuraminic acid, along with 8-O-methyl-2-keto-3-deoxy-d-glycero-d-galacto-nonulosonic acid (Kdn8Me) and 8-deoxy-Kdn were synthesized from corresponding 5-O-modified six-carbon monosaccharides and pyruvate using a sialic acid aldolase cloned from Pasteurella multocida strain P-1059 (PmNanA). In addition, α2-3- and α2-6-linked sialyltrisaccharides containing Neu5Ac8Me and Kdn8Deoxy were also synthesized using a one-pot multienzyme approach. The strategy reported here provides an efficient approach to produce glycans containing various C8-modified sialic acids for biological evaluations.
authors: Department of Chemistry, University of California, One Shields Avenue, Davis, CA 95616, United States.
authors: Hai
authors: H
authors: Yu
authors: Hongzhi
authors: H
authors: Cao
authors: Vinod Kumar
authors: VK
authors: Tiwari
authors: Yanhong
authors: Y
authors: Li
authors: Xi
authors: X
authors: Chen
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