title: C-type lectins on macrophages participate in the immunomodulatory response to Fasciola hepatica products.
abstract: Fasciola hepatica releases excretory-secretory products (FhESP), and immunomodulatory properties have been described for the carbohydrates present in these parasite products. The interaction of FhESP with the innate immune cells, such as macrophages, is crucial in the early stage of infection. In this work we observed that peritoneal macrophages from naive BALB/c mice stimulated in vitro with FhESP presented: an increased arginase activity as well as Arginase I expression, and high levels of transforming growth factor-β and interleukin-10. A similar macrophage population was also observed in the peritoneum of infected mice. A partial inhibition of the immunomodulatory effects described above was observed when macrophages were pre-incubated with Mannan, anti-mannose receptor, Laminarin or anti-Dectin-1, and then stimulated with FhESP. In addition, we observed a partial inhibition of these effects in macrophages obtained from mice that were intraperitoneally injected with Mannan or Laminarin before being infected. Taken together, these results suggest the participation of at least two C-type lectin receptors, mannose receptor and Dectin-1, in the interaction of FhESP with macrophages, which allows this parasite to induce immunoregulatory effects on these important innate immune cells and may constitute a crucial event for extending its survival in the host.
authors: Centro de Investigaciones en Bioquímica Clínica e Inmunología, CONICET, Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Medina Allende y Haya de la Torre, Ciudad Universitaria, Argentina.
authors: Lorena
authors: L
authors: Guasconi
authors: Marianela C
authors: MC
authors: Serradell
authors: Ana P
authors: AP
authors: Garro
authors: Luciana
authors: L
authors: Iacobelli
authors: Diana T
authors: DT
authors: Masih
references: J Immunol. 2007 Mar 1;178(5):3107-15
references: 17312158
references: Biochem Soc Trans. 1991 Feb;19(1):27S
references: 2037164
references: Eur J Immunol. 2009 Feb;39(2):507-13
references: 19130473
references: Eur J Immunol. 2007 Jan;37(1):14-6
references: 17183610
references: J Leukoc Biol. 2007 May;81(5):1188-96
references: 17261547
references: Vet Immunol Immunopathol. 2007 Jun 15;117(3-4):197-208
references: 17449115
references: Clin Immunol Immunopathol. 1998 May;87(2):145-54
references: 9614929
references: J Biol Chem. 2000 Jun 30;275(26):20157-67
references: 10779524
references: Vet Immunol Immunopathol. 2007 Nov 15;120(1-2):31-40
references: 17719651
references: Blood. 2005 Oct 1;106(7):2543-50
references: 15956283
references: J Parasitol. 1998 Feb;84(1):55-61
references: 9488338
references: J Exp Med. 2009 Aug 3;206(8):1673-80
references: 19635864
references: J Biol Chem. 1994 Jun 3;269(22):15512-9
references: 8195195
references: Vet Parasitol. 1996 Jan;61(1-2):97-111
references: 8750687
references: Vet Parasitol. 1982 Nov;11(2-3):165-78
references: 6761952
references: Immunity. 2005 Apr;22(4):507-17
references: 15845454
references: FASEB J. 2008 Nov;22(11):4022-32
references: 18708590
references: J Immunol Methods. 1994 Sep 14;174(1-2):231-5
references: 8083527
references: J Exp Med. 1999 Jun 21;189(12):1961-72
references: 10377192
references: Mol Biol Cell. 2005 Feb;16(2):824-34
references: 15574879
references: Cell Microbiol. 2008 Oct;10(10):2058-66
references: 18549457
references: Parasitol Res. 2006 Sep;99(4):440-8
references: 16598470
references: Infect Immun. 2003 Jan;71(1):437-45
references: 12496194
references: Clin Exp Immunol. 1980 Sep;41(3):430-40
references: 7438560
references: Infect Immun. 2005 Jan;73(1):166-73
references: 15618151
references: J Biol Chem. 2002 Nov 1;277(44):41613-23
references: 12196537
references: Cell Microbiol. 2008 Aug;10(8):1608-21
references: 18373632
references: Immunol Rev. 1998 Jun;163:19-34
references: 9700499
references: J Immunol. 1996 Nov 15;157(10):4568-75
references: 8906835
references: Vet Immunol Immunopathol. 1996 Oct;53(3-4):257-68
references: 8969046
references: Immunol Rev. 2009 Jul;230(1):247-57
references: 19594641
references: Infect Immun. 2008 Feb;76(2):678-84
references: 18070895
references: J Immunol. 2003 Nov 1;171(9):4552-60
references: 14568928
references: Nat Rev Immunol. 2008 Dec;8(12):958-69
references: 19029990
references: World J Gastroenterol. 2008 Sep 7;14(33):5125-32
references: 18777588
references: Ann N Y Acad Sci. 2008 Nov;1143:45-60
references: 19076344
references: Nat Rev Immunol. 2003 Jan;3(1):23-35
references: 12511873
references: Nature. 1992 Nov 12;360(6400):183-6
references: 1279438
references: Parasitology. 1999 Dec;119 ( Pt 6):627-33
references: 10633925
references: Annu Rev Immunol. 2009;27:451-83
references: 19105661
references: Int J Parasitol. 2010 Aug 1;40(9):1051-62
references: 20361966
references: Immunology. 2009 Feb;126(2):246-55
references: 18624733
references: J Exp Med. 2002 Aug 5;196(3):407-12
references: 12163569
references: Blood. 2008 Apr 15;111(8):4264-72
references: 17698636
references: Mol Immunol. 2004 Nov;41(11):1109-21
references: 15476922
references: J Immunol. 2002 Oct 1;169(7):3876-82
references: 12244185
references: Immunobiology. 2001 Dec;204(5):527-35
references: 11846215
references: Infect Immun. 2003 Nov;71(11):6213-21
references: 14573639
references: Nature. 2006 Aug 10;442(7103):651-6
references: 16862125
references: Nat Rev Immunol. 2006 Jan;6(1):33-43
references: 16341139
references: J Pharmacol Exp Ther. 2006 Aug;318(2):540-6
references: 16632639
references: Vet Immunol Immunopathol. 2006 Jan 15;109(1-2):57-67
references: 16135385
references: J Immunol. 2008 Sep 1;181(5):3549-57
references: 18714028
references: J Biol Chem. 2001 Nov 23;276(47):43818-23
references: 11567029
references: J Immunol. 2009 Aug 1;183(3):1577-86
references: 19587018
references: Blood. 2006 Nov 1;108(9):3168-75
references: 16825490
references: Nat Immunol. 2007 Jun;8(6):630-8
references: 17450144
references: J Immunol. 2007 Sep 15;179(6):3463-71
references: 17785780
references: Nature. 2001 Sep 6;413(6851):36-7
references: 11544516
references: Immunol Rev. 2004 Oct;201:89-116
references: 15361235
references: Int J Parasitol. 1997 May;27(5):573-9
references: 9193951
references: Int J Parasitol. 2001 Nov;31(13):1467-73
references: 11595234
references: J Exp Med. 1995 Nov 1;182(5):1243-58
references: 7595195
references: Eur J Immunol. 2003 Feb;33(2):483-93
references: 12645947
references: Curr Opin Immunol. 2005 Feb;17(1):18-24
references: 15653305