title: Simultaneous quantification of Echinacea species, Flos Lonicerae, Radix Scutellaria and Fructus Forsythiae combinations by rapid resolution liquid chromatography.
abstract: Echinacea angustifolia and E. purpurea are commonly used in North America for their anti-bacterial effects. Flos Lonicerae, Radix Scutellaria and Fructus Forsythiae are traditional Chinese medicinal herbs commonly used for the treatment of complaints such as pneumonia, acute upper respiratory tract infection, and acute bronchitis. A reproducible, simple, and reliable rapid resolution liquid chromatographic (RRLC) method has been developed to analyze extracts of products formulated containing E. angustifolia, E. purpurea, Flos Lonicerae, Radix Scutellariae and Fructus Forsythiae simultaneously in one run in less than 6 minutes. The method uses a C18-HST column, a mobile phase consisting of 0.1% aqueous phosphoric acid solution and acetonitrile, and UV detection at 327 nm and 229 nm. A stability test was performed that revealed that chlorogenic acid is more stable in acidic pH, and hence it is best to keep the extract of E. augustifolia, E. purpurea, Flos Lonicerae, Radix Scutellariae and Fructus Forsythiae in mild acidic conditions at approximately pH 5.
authors: Canadian Phytopharmaceuticals Corp., 12233 Riverside Way, Richmond, B.C., Canada V6W 1K8.
authors: Jie
authors: J
authors: Ma
authors: Yuan-chun
authors: YC
authors: Ma
authors: Carina
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authors: Cai
authors: Daniel
authors: D
authors: Wang
authors: Fei Fei
authors: FF
authors: Hou
authors: Mai
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authors: Luo
authors: Shane
authors: S
authors: Lu
authors: Dariusz C
authors: DC
authors: Gorecki
authors: Asmita V
authors: AV
authors: Patel
authors: Alice
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authors: Chen
authors: Peter
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authors: Jin