title: Optimum conditions for serum clearance of iodixanol, applicable to the estimation of glomerular filtration rate in horses.
abstract: To estimate the glomerular filtration rate (GFR) in horses, an optimum dose of the nonionic contrast medium iodixanol as a tracer was assessed with blood-sample times. Iodixanol was administered intravenously at 10-40 mg I/kg to geldings and mares, and blood was collected 30, 60, 90, 120, 150, and 180 min later. Serum iodixanol concentration was determined by high-performance liquid chromatography (HPLC), and serum urea nitrogen (UN) and creatinine concentrations were also measured. The combination of 20 mg I/kg iodixanol and sampling times of 60, 90, and 120 min after injection was considered to be appropriate for practical use. In clinically healthy horses, the reference values were determined to be 1.90 ± 0.03 ml/min/kg (150.8 ± 2.94 ml/min/m2), consistent with historical data using different tracers. The result suggests that serum clearance of iodixanol is a ready-to-use tool for a screening of alterations in the equine GFR, although it is necessary to perform a more longitudinal study using horses with a variety of renal functions.
authors: Shinwa BioCraft Laboratory Inc., 1-24-50 Edogawa, Edogawa-ku, Tokyo, 132-0013, Japan.
authors: Hiroshi
authors: H
authors: Satoh
authors: Sumito
authors: S
authors: Abe
authors: Masaki
authors: M
authors: Kato
authors: Jun
authors: J
authors: Saito
authors: Kazuhisa
authors: K
authors: Furuhama
references: Scand J Clin Lab Invest. 1972 Nov;30(3):271-4
references: 4629674
references: Acta Radiol Suppl. 1995;399:69-82
references: 8610532
references: J Pharmacol Toxicol Methods. 2010 Jan-Feb;61(1):59-64
references: 19854282
references: Clin Exp Nephrol. 2007 Mar;11(1):41-50
references: 17384997
references: Am J Vet Res. 1992 Sep;53(9):1612-6
references: 1416364
references: Equine Vet J. 2000 Nov;32(6):509-14
references: 11093625
references: Acta Radiol Suppl. 1995;399:61-6
references: 8610531
references: J Urol. 1991 Sep;146(3):675-9
references: 1875470
references: Am J Vet Res. 1982 Apr;43(4):608-12
references: 7073082
references: Am J Physiol. 1968 Sep;215(3):704-15
references: 5671010
references: Am J Vet Res. 2009 Dec;70(12):1545-50
references: 19951127
references: J Am Coll Cardiol. 2006 Aug 15;48(4):692-9
references: 16904536
references: Vet J. 1999 Nov;158(3):204-9
references: 10558840
references: N Engl J Med. 2003 Feb 6;348(6):491-9
references: 12571256
references: J Vet Pharmacol Ther. 1988 Dec;11(4):409-12
references: 3210269
references: Am J Vet Res. 1992 May;53(5):776-80
references: 1524306