title: Determination of melamine in milk powder based on the fluorescence enhancement of Au nanoparticles.
abstract: We have developed a new analytical method to detect melamine (MA) in milk powder based on the fluorescence enhancement of Au nanoparticles (AuNPs). AuNPs with the average diameter of ∼16 nm can emit stable fluorescence at 370 nm when the excitation wavelength was selected at 252 nm. The AuNPs could assemble with melamine to form larger aggregates (AuNPs-MA) through electrostatic interaction and coordinating interaction in acidic conditions, which led to the significant enhancement of the fluorescence intensity. Under the optimized conditions, the enhancement of the fluorescence intensity exhibited a good linear dependence on melamine concentration in the range from 8.0 × 10(-10) to 8.0 × 10(-8) M, and the detection limit is 6.1 × 10(-10) M (3σ). This proposed method showed high precision and accuracy when applied to the real sample analyses. In conclusion, a simple, rapid, accurate and sensitive method to detect melamine has been suggested.
authors: Key Laboratory of Analytical Chemistry for Biology and Medicine, Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University, China.
authors: Dongshan
authors: D
authors: Xiang
authors: Guoping
authors: G
authors: Zeng
authors: Kun
authors: K
authors: Zhai
authors: Li
authors: L
authors: Li
authors: Zhike
authors: Z
authors: He