title: [A cross-sectional survey of occult hepatitis B virus infection in HIV-infected patients in acquired immune deficiency syndrome area].
abstract: OBJECTIVE
  To assess the prevalence of occult HBV infection in HIV-infected patients inacquired immune deficiency syndrome area.
  
  
  METHODS
  Serum samples were obtained from 97 HIV-infected patients who transmitted by paid blood donation. ELISA was used to detect HBV erologic markers (HBsAg, Anti-HBs, HBeAg, anti-HBe and anti-HBc) and HCV antibody. Flow Cytometry were used to detect CD4+ T cell count. Nested PCR was used to amplify surface protein region of HBV DNA.
  
  
  RESULTS
  Ninety two patients were HBsAg negative in the 97 HIV-infected patients (94.85%). Twenty seven patients were co-infected with occult hepatitis B virus infection in the 92 HBsAg negative patients (29.35%). Seventy three patients were co-infected with HCV in the 92 HBsAg negative patients(79.35%). CD4 cell count of subjects with occult HBV infection were significantly lower (212.11 +/- 133.1 cells/mm3 versus 318.9 +/- 172.2 cells/mm3, respectively, P < 0.01). A significantly higher prevalence of isolated anti-HBc was observed in HIV-infected subjects co-infectioned with occult HBV infection [62.96% (13 of 27) versus 18.46% (15 of 65), P < 0.01]. No statistical significant association could be established between the age, sex and whether co-infected with HCV.
  
  
  CONCLUSIONS
  It is found that occult HBV infection did occurs in HIV-infected patients. Individuals co-infected with HIV and occult HBV infection are more likely to have isolated anti-HBc than subjects with HIV alone. Co-infection with HIV and occult HBV is more likely to occue in subjects with lower CD4.
authors: Department of Infectious Disease, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
authors: Hong-Xia
authors: HX
authors: Liang
authors: Yuan-Yuan
authors: YY
authors: Chen
authors: Rong
authors: R
authors: Zhou
authors: Qian
authors: Q
authors: Zhang
authors: Yan-Feng
authors: YF
authors: Pan
authors: Jun-Sheng
authors: JS
authors: Gu
authors: Juan
authors: J
authors: Li
authors: He-Qing
authors: HQ
authors: Jiang
authors: Zu-Jiang
authors: ZJ
authors: Yu