title: Ogataea saltuana sp. nov., a novel methanol-assimilating yeast species.
abstract: Four ascosporulating strains of an undescribed methanol-assimilating yeast species were isolated from forest habitats in Hungary. Three were recovered from rotten wood and one from leaves of a sessile oak (Quercus petraea). An additional isolate of the undescribed species sharing similar phenotypic characters with the above-noted strains was recovered from the gut of an unidentified beetle collected from under the bark of a coniferous tree in Bulgaria. A closely related, but somewhat divergent strain was recovered from insect frass in a Ponderosa pine (Pinus ponderosa) collected in New Mexico, USA. Analysis of the D1/D2 sequences of the LSU rRNA gene placed the new species in the Ogataea clade. The ITS and the D1/D2 LSU sequences of the rRNA gene repeats were compared for the above-noted strains and that of the type strain of Ogataea zsoltii, the closest neighbour among currently recognized Ogataea species. Their relatedness was investigated by parsimony network analysis as well. As a result of the sequence analysis, it was concluded that the six strains isolated from tree associated habitats represent a single new yeast species. Ogataea saltuana sp. nov. is proposed to accommodate these strains. The type strain NCAIM Y.01833(T) (CBS 10795(T), NRRL Y-48448(T)) was recovered from rotten wood of Scotch pine (Pinus silvestris) in Hungary. The GenBank accession number for the D1/D2 domain nuclear large subunit rRNA gene sequence of strain NCAIM Y.01833(T) (CBS 10795(T), NRRL Y-48448(T)) is EU327033. The MycoBank number of the new species is MB 519966.
authors: National Collection of Agricultural and Industrial Microorganisms, Faculty of Food Sciences, Corvinus University of Budapest, Somlói út 14-16, Budapest, Hungary. gabor.peter@uni-corvinus.hu
authors: Gábor
authors: G
authors: Péter
authors: Dénes
authors: D
authors: Dlauchy
authors: Judit
authors: J
authors: Tornai-Lehoczki
authors: Dilnora
authors: D
authors: Gouliamova
authors: Cletus P
authors: CP
authors: Kurtzman