title: The genes of the alpha-chain and Beta-chain of the gm-csp receptor are not rearranged in patients with aml.
abstract: Recently alterations in growth factor receptors, e.g. the erythropoietin receptor, have been described which cause increased sensitivity to growth factors or even autonomous growth independent from exogenous stimuli. We therefore investigated the gene structure of the alpha and beta chains of the GM-CSF receptor using Southern blots. EcoRI and HindIII digested DNA from peripheral blood samples from 34 consecutive patients with AML were hybridized with probes specific for the GM-CSF receptor alpha and beta chains. No abnormal DNA was detected. RNA expression was investigated by Northern blots in 32 patients. Expression of the beta chain was found in 30 of 32 patients. No messenger RNAs of aberrant size were detected. PCR reactions were performed with oligonucleotide primers specific for the alpha chain; c-DNAs were identified in all 10 patients studied. Thus we conclude that gene structure and expression of both chains of the GM-CSF receptor are not altered in AML.
authors: W
authors: W
authors: Fiedler
authors: A
authors: A
authors: Samalecos
authors: D
authors: D
authors: Hossfeld