title: Phylogeography and genetic structuring of European nine-spined sticklebacks (Pungitius pungitius)-mitochondrial DNA evidence.
abstract: As a consequence of colonisation from different glacial refugia, many northern European taxa are split into distinct western and eastern lineages. However, as for the nine-spined stickleback (Pungitius pungitius), the exact location of the contact zone between lineages often remains poorly known. We assessed the genetic differentiation and diversity in the nine-spined stickleback within Europe using 1037 base pairs of cytochrome b sequence for 320 individuals from 57 locations, including pond, lake, river, and coastal habitats. Our main aims were (i) to locate the contact zone between the previously recognized western and eastern lineages, (ii) investigate latitudinal patterns in genetic diversity, (iii) compare genetic diversity among different habitat types, and (iv) date the known split between eastern and western lineages. The data revealed the split between eastern and western to be located across the Danish Straits and roughly following the Norway/Sweden border to the North. Reference sites from Canada form their own clades, and one of the Canadian sites was found to have a haplotype common to the Eastern European lineage, possibly representing an ancestral polymorphism. The split between the two European clades was dated to approximately 1.48 million years ago (Mya), and between Canada and Europe to approximately 1.62 Mya. After controlling for habitat effects, nucleotide (but not haplotype) diversity across populations decreased with increasing latitude. Coastal populations showed significantly higher haplotype diversity (but not nucleotide diversity) than pond populations, but there were no detectable differences in haplotype diversity among different freshwater habitat types (viz. river, lake and pond populations), or between coastal and lake/river populations. Sequences were found to cluster according to their geographic proximity, rather than by habitat type, and all habitat types were found within each major clade, implying that colonisation and adaptation between the coastal and freshwater environments in different regions must have occurred in parallel.
authors: Ecological Genetics Research Unit, Department of Biosciences, University of Helsinki, Helsinki, Finland. amber.teacher@helsinki.fi
authors: Amber G F
authors: AG
authors: Teacher
authors: Takahito
authors: T
authors: Shikano
authors: Marika E
authors: ME
authors: Karjalainen
authors: Juha
authors: J
authors: Merilä
references: Philos Trans R Soc Lond B Biol Sci. 2004 Feb 29;359(1442):183-95; discussion 195
references: 15101575
references: Mol Ecol. 2006 Jul;15(8):2013-29
references: 16780421
references: Nature. 2000 Jun 22;405(6789):907-13
references: 10879524
references: Mol Phylogenet Evol. 2008 Jan;46(1):167-82
references: 17716925
references: Mol Ecol. 1998 Apr;7(4):453-64
references: 9628000
references: Mol Ecol. 2010 Sep;19(18):4061-76
references: 20854276
references: Bioinformatics. 2001 Aug;17(8):754-5
references: 11524383
references: BMC Evol Biol. 2007 Nov 08;7:214
references: 17996036
references: Genetika. 2006 Oct;42(10):1319-34
references: 17152703
references: Mol Ecol. 2001 Jan;10(1):89-102
references: 11251790
references: PLoS Genet. 2010 Feb 26;6(2):e1000862
references: 20195501
references: Mol Ecol. 1995 Jun;4(3):285-97
references: 7663748
references: Mol Ecol. 2000 Oct;9(10):1609-24
references: 11050556
references: Genetics. 2002 Jul;161(3):1307-20
references: 12136032
references: Mol Ecol. 2010 Nov;19(22):4852-62
references: 20849560
references: Mol Ecol. 2001 Aug;10(8):1983-2002
references: 11555242
references: Mol Ecol. 2006 May;15(6):1519-34
references: 16629808
references: Heredity (Edinb). 2002 May;88(5):391-401
references: 11986877
references: Mol Ecol. 2010 Mar;19(6):1147-61
references: 20163545
references: Bioinformatics. 2009 Jun 1;25(11):1451-2
references: 19346325
references: Mol Biol Evol. 2005 Jul;22(7):1561-8
references: 15814826
references: Mol Ecol. 1999 Sep;8(9):1387-404
references: 10564445
references: Syst Biol. 2003 Oct;52(5):696-704
references: 14530136
references: Brief Bioinform. 2008 Jul;9(4):299-306
references: 18417537
references: Genetics. 1981 Jan;97(1):145-63
references: 6266912