title: High throughput miniaturization of artificial cells.
abstract: In this study, inkjet bio-printing has been used to produce miniaturized alginate microcapsules. A parametric study using subsequent Taguchi L(18) (3(1) × 2(7)) and L(16) (4(5)) designs was performed to elucidate the effect of inkjet parameters on microcapsule size. A 120-minute pilot run using the optimal waveform parameters and 0.5% alginate ink yielded a throughput of 1.8×10(6) microcapsules/hr, averaging 40 μm in diameter. Real-time stable jetting conditions were confirmed visually by the generation of a single droplet with a straight trajectory and non-fluctuating Ohnesorge numbers. The rate of stirring of the cross-linking CaCl(2) solution determined scaffold vs. single vesicle formation.
authors: San Jose State University, CA, USA. maryam.mobed-miremadi@sjsu.edu
authors: Maryam
authors: M
authors: Mobed-Miremadi
authors: Erik
authors: E
authors: Acks
authors: Sutthipong
authors: S
authors: Polsaward
authors: Ding
authors: D
authors: Chen