title: Direct correlation between IL-2-R gene-transcription and natural cell-mediated cytotoxicity in patients with gastric-carcinoma.
abstract: The relationship between IL-2-R gene transcription and natural cell mediated cytotoxicity (NKCMC) in the peripheral blood lymphocytes of 15 patients with gastric carcinoma and 6 normal healthy controls were studied. NKCMC and ADCC assessed against radiolabelled K-562 and sensitized chicken RBC as respective target cells in Cr-51 release assay revealed significant reduction in patients as compared to controls. The impairment in the natural cytotoxicity was associated with decreased IL-2-R gene expression at the mRNA level in these patients. Expression of both IL-2 R alpha transcripts (3.5 and 1.5 Kb) were either not detectable or only weakly detectable on T lymphocytes from patients even after mitogenic stimulation. In contrast, a significant rise in the expression of both IL-2 R alpha transcripts was observed on T cells from normal controls followed by mitogenic challenge. We interpret our findings to reflect the direct correlation between NKCMC and IL-2-R gene transcription in gastric carcinoma patients.
authors: COLL MED,DEPT BIOCHEM,TRICHUR 680596,KERALA,INDIA. INST CANC RES,MADRAS 600020,TAMIL NADU,INDIA.
authors: K
authors: K
authors: Suresh
authors: K
authors: K
authors: Jayavardhanan
authors: K
authors: K
authors: Nirmala
authors: D
authors: D
authors: Vasudevan