title: Bovine IFNGR2, IL12RB1, IL12RB2, and IL23R polymorphisms and MAP infection status.
abstract: Mycobacterium avium ssp. paratuberculosis (MAP) infection causes a chronic granulomatous inflammatory condition of the bovine gut that is characterized by diarrhea, progressive weight loss, and emaciation, and ultimately leads to loss in productivity and profitability of dairy operations. The host cytokine machinery is known to play an important role in protecting against MAP infection. Therefore, the goal of the present study was to assess whether polymorphisms in candidate genes encoding important cytokines and cytokine receptors are associated with MAP infection status of dairy cattle. MAP infection status was evaluated based on serum and milk enzyme-linked immunosorbent assays (ELISAs) for MAP-specific antibodies. Twenty previously reported polymorphisms in genes encoding bovine interferon gamma (IFNG), IFNGR1, IFNGR2, IL22, IL22RA1, IL12RB1, IL12RB2, and IL23R were genotyped in a resource population of 446 dairy Holsteins with known MAP infection status, and logistic regression was used to assess the statistical association with a binomial MAP infection status phenotype. Four SNPs in IFNGR2, IL12RB1, IL12RB2, and IL23R were found to be associated with the MAP infection status of the resource population. These results underscore the importance of cytokines and their receptors in conferring protection against MAP infection and warrant further functional characterization of these associations.
authors: Department of Animal and Poultry Science, Centre for Genetic Improvement of Livestock, University of Guelph, ON N1G 2W1, Canada.
authors: Sameer D
authors: SD
authors: Pant
authors: Chris P
authors: CP
authors: Verschoor
authors: Alicia M
authors: AM
authors: Skelding
authors: Flavio S
authors: FS
authors: Schenkel
authors: Qiumei
authors: Q
authors: You
authors: Graham A
authors: GA
authors: Biggar
authors: David F
authors: DF
authors: Kelton
authors: Niel A
authors: NA
authors: Karrow
references: Vet Immunol Immunopathol. 2009 Feb 15;127(3-4):235-41
references: 19027177
references: Nat Rev Immunol. 2003 Feb;3(2):133-46
references: 12563297
references: Science. 2007 Jan 26;315(5811):525-8
references: 17185560
references: J Dairy Sci. 1998 Nov;81(11):3029-35
references: 9839242
references: J Dairy Sci. 2000 Nov;83(11):2702-8
references: 11104291
references: J Appl Microbiol. 2005;98(6):1282-93
references: 15916642
references: Int Immunopharmacol. 2004 May;4(5):669-77
references: 15120651
references: J Dairy Sci. 2006 Feb;89(2):569-79
references: 16428626
references: Nat Genet. 2004 Apr;36(4):388-93
references: 15052270
references: J Dairy Sci. 2006 May;89(5):1804-12
references: 16606752
references: J Exp Med. 1993 Dec 1;178(6):2243-7
references: 8245795
references: Nature. 2007 Feb 8;445(7128):648-51
references: 17187052
references: Prev Vet Med. 2009 May 1;89(1-2):102-9
references: 19327856
references: Science. 2006 Dec 1;314(5804):1461-3
references: 17068223
references: J Dairy Sci. 2004 Jul;87(7):2108-13
references: 15328223
references: Infect Immun. 1988 Jul;56(7):1692-7
references: 2838423
references: Methods Mol Biol. 2009;578:23-39
references: 19768585
references: Anim Genet. 2009 Oct;40(5):655-62
references: 19422364
references: J Dairy Res. 2002 Nov;69(4):645-9
references: 12463700
references: Infect Immun. 2004 Jun;72(6):3089-96
references: 15155609
references: Int J Mol Med. 2011 Mar;27(3):469-77
references: 21206965
references: Inflamm Bowel Dis. 2009 Jun;15(6):883-9
references: 19235914
references: Prev Vet Med. 1997 Oct;32(3-4):253-66
references: 9443332
references: Vet Microbiol. 2008 Jun 22;129(3-4):217-35
references: 18255239
references: J Immunol. 2004 Jan 1;172(1):61-9
references: 14688310
references: Trends Immunol. 2006 Jan;27(1):17-23
references: 16290228
references: J Exp Med. 1993 Dec 1;178(6):2249-54
references: 7504064
references: Vet Q. 2007 Dec;29(4):138-48
references: 18265703
references: Hum Hered. 2005;59(3):165-75
references: 15920341
references: Prev Vet Med. 2000 Jul 3;46(1):15-27
references: 10854933
references: Prev Vet Med. 2007 Oct 16;81(4):306-17
references: 17561292
references: J Dairy Sci. 2004 Feb;87(2):443-9
references: 14762087
references: J Exp Med. 2006 Oct 2;203(10):2271-9
references: 16982811
references: J Immunol. 2004 Jan 1;172(1):525-31
references: 14688363
references: Immunol Rev. 2008 Dec;226:132-46
references: 19161421