title: Immunoassay based on monoclonal antibodies versus LC-MS: deoxynivalenol in wheat and flour in Southern Brazil.
abstract: An indirect competitive enzyme-linked immunosorbent assay (ELISA) method using a monoclonal antibody for deoxynivalenol (DON) detection in wheat and flour was standardised and validated (detection limit = 177.1 µg kg(-1)) and its performance was compared with LC-MS, quantification limit =140 µg kg(-1)). DON recovery ranged from 88.7% to 122.6% for wheat grain and from 70.6% to 139.3% for flour. Among the 38 wheat samples evaluated, DON was detected in 29 samples (76.3%) by ic-ELISA (281.6-12 291.4 µg kg(-1)) and in 22 samples (57.9%) by LC-MS (155.3-9906.9 µg kg(-1)). The 0.93 correlation coefficient between ic-ELISA and LC-MS data in 19 positive DON wheat samples demonstrated the reliability and efficiency of ic-ELISA. Results indicated that standardised ic-ELISA was suitable for DON screening in wheat samples and the need for continuous monitoring of mycotoxin levels in foodstuffs.
authors: Department of Food Science and Technology, Center of Agrarian Sciences, State University of Londrina, 86051-980, Londrina, Paraná, Brazil. joice.sifuentes@gmail.com
authors: J Sifuentes
authors: JS
authors: Dos Santos
authors: C R
authors: CR
authors: Takabayashi
authors: E Y S
authors: EY
authors: Ono
authors: E N
authors: EN
authors: Itano
authors: C A
authors: CA
authors: Mallmann
authors: O
authors: O
authors: Kawamura
authors: E Y
authors: EY
authors: Hirooka