title: Identification of the phosphorylation sites in the survival motor neuron protein by protein kinase A.
abstract: The survival motor neuron (SMN) protein plays an essential role in the assembly of uridine-rich small nuclear ribonuclear protein complexes. Phosphorylation of SMN can regulate its function, stability, and sub-cellular localization. This study shows that protein kinase A (PKA) phosphorylates SMN both in vitro and in vivo. Bioinformatic analysis predicts 12 potential PKA phosphorylation sites in human SMN. Mass spectrometric analysis of a tryptic digest of SMN after PKA phosphorylation identified five distinct phosphorylation sites in SMN (serines 4, 5, 8, 187 and threonine 85). Mutagenesis of this subset of PKA-phosphorylated sites in SMN affects association of SMN with Gemin2 and Gemin8. This result indicates that phosphorylation of SMN by PKA may play a role in regulation of the in vivo function of SMN.
authors: Department of Biological Sciences, University of Delaware, Newark, DE, USA. wcy@UDel.Edu
authors: Chia-Yen
authors: CY
authors: Wu
authors: Amelia
authors: A
authors: Curtis
authors: Yong Seok
authors: YS
authors: Choi
authors: Miho
authors: M
authors: Maeda
authors: Mary Jue
authors: MJ
authors: Xu
authors: Amanda
authors: A
authors: Berg
authors: Upasana
authors: U
authors: Joneja
authors: Robert W
authors: RW
authors: Mason
authors: Kelvin H
authors: KH
authors: Lee
authors: Wenlan
authors: W
authors: Wang
references: BMC Cell Biol. 2009 May 16;10:40
references: 19445707
references: Results Probl Cell Differ. 2009;48:289-326
references: 19343312
references: Mol Cell Proteomics. 2009 Jul;8(7):1751-64
references: 19369195
references: Mol Cell Biol. 1993 Jul;13(7):4157-66
references: 8321220
references: Mol Genet Metab. 2005 May;85(1):38-45
references: 15862279
references: BMC Neurosci. 2011 Mar 08;12:25
references: 21385431
references: J Cell Biol. 2007 Nov 5;179(3):451-65
references: 17984321
references: Mol Cell Biol. 2009 Mar;29(5):1107-15
references: 19103745
references: EMBO Rep. 2007 Apr;8(4):340-5
references: 17401408
references: EMBO Rep. 2005 Jan;6(1):70-6
references: 15592453
references: Bioessays. 2010 Dec;32(12):1077-89
references: 20954180
references: Lancet. 2008 Jun 21;371(9630):2120-33
references: 18572081
references: Biochem J. 2007 Dec 15;408(3):297-315
references: 17850214
references: Nat Rev Neurosci. 2009 Aug;10(8):597-609
references: 19584893