title: Preparation and anti-bacterial properties of a temperature-sensitive gel containing silver nanoparticles.
abstract: The purpose of this study was to prepare a temperature-sensitive gel containing silver nanoparticles and to investigate its anti-bacterial properties in vitro. The aqueous gel was prepared using Pluronic F127 (18-22%) and Pluronic F68 (3-9%) in a cold method to obtain a proper gelation temperature at 37 degrees C. Viscoelastic properties of the system were measured by rheological measurements and the physicochemical properties were evaluated by MJ-22 Dial-reflex metaloscope and Zetasizer Nano ZS90. The in vitro antimicrobial activity was evaluated by a disk diffusion test, minimum inhibitory concentration, and minimum bactericidal concentration. A temperature-sensitive gel containing silver nanoparticles with 20 wt% F127 and 6 wt% F68 had suitable fluidity at 25 degrees C and was semi-solid at 37 degrees C. Silver nanoparticle size averaged 78.0 nm. The gel optimized formulation achieved a suitable viscosity. The MIC and MBC of the gel ranged from 1.0 to 2.0 mg/L against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. The activity of the gel against these three species was significantly enhanced (p<0.05) compared to 400 mg/L Asimi standard. This optimized silver nanoparticle dosage form demonstrated a high potential for further development for the clinical treatment of bacterial vaginosis.
authors: The School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, China.
authors: Meiwan
authors: M
authors: Chen
authors: Xin
authors: X
authors: Pan
authors: Hongmei
authors: H
authors: Wu
authors: Ke
authors: K
authors: Han
authors: Xiaobao
authors: X
authors: Xie
authors: D E
authors: DE
authors: Wedge
authors: M A
authors: MA
authors: Repka
authors: Chuanbin
authors: C
authors: Wu