title: Matrix-assisted laser desorption/ionization imaging mass spectrometry.
abstract: Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is a powerful tool that enables the simultaneous detection and identification of biomolecules in analytes. MALDI-imaging mass spectrometry (MALDI-IMS) is a two-dimensional MALDI-mass spectrometric technique used to visualize the spatial distribution of biomolecules without extraction, purification, separation, or labeling of biological samples. MALDI-IMS has revealed the characteristic distribution of several biomolecules, including proteins, peptides, amino acids, lipids, carbohydrates, and nucleotides, in various tissues. The versatility of MALDI-IMS has opened a new frontier in several fields such as medicine, agriculture, biology, pharmacology, and pathology. MALDI-IMS has a great potential for discovery of unknown biomarkers. In this review, we describe the methodology and applications of MALDI-IMS for biological samples.
authors: Department of Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan; E-Mails: thaya@hama-med.ac.jp (T.H.); naoko.goto.inoue@gmail.com (N.G.-I.); setou@hama-med.ac.jp (M.S.).
authors: Nobuhiro
authors: N
authors: Zaima
authors: Takahiro
authors: T
authors: Hayasaka
authors: Naoko
authors: N
authors: Goto-Inoue
authors: Mitsutoshi
authors: M
authors: Setou
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