title: [Development of an amplified luminescent proximity homogeneous immunoassay kit for detecting human hepatitis B virus e antibody].
abstract: OBJECTIVE
  To develop an amplified luminescent proximity homogeneous immunoassay (AlphaLISA) kit for the detection of human hepatitis B virus e antibody (HBeAb).
  
  
  METHODS
  The neutralizing and competitive inhibition method was used to develop the AlphaLISA kit for detection of serum HBeAb.
  
  
  RESULTS
  The working range of the kit was 0.003-16 NCU/ml with a sensitivity up to 0.003 NCU/ml. The intra- and inter-assay coefficient of variation was 5.3% and 6.8%, respectively. The kit showed no cross-reaction with HBcAb, and comparison of the detection results with those of a commercially available Elecsys HBeAb kit (Roche) for 136 samples showed a correlation coefficient of 0.961.
  
  
  CONCLUSIONS
  The AlphaLISA kit for HBeAb detection meets the clinical requirements for detection HBeAb in human serum.
authors: College of Biotechnology, Southern Medical University, Guangzhou, China. mqqqm2006@163.com
authors: Qiang
authors: Q
authors: Ma
authors: An
authors: A
authors: He
authors: Zhi-ning
authors: ZN
authors: Dong
authors: Tian-cai
authors: TC
authors: Liu
authors: Li-ping
authors: LP
authors: Zou
authors: Guan-feng
authors: GF
authors: Lin
authors: Ming
authors: M
authors: Li
authors: Ying-song
authors: YS
authors: Wu