title: A novel in vitro model for preclinical testing of the hemocompatibility of intravascular stents according to ISO 10993-4.
abstract: Subacute stent thrombosis, caused by undesired interactions between blood and the stent surface, is a major concern in the first few weeks following coronary artery stent implantation. The aim of this study was to establish a novel in vitro model for hemocompatibility testing of coronary artery stents according to ISO 10993-4. The model consists of a modified Chandler-Loop design with closed heparin-coated PVC Loops and a thermostated water bath. The tests were performed with anticoagulated human whole blood. After incubation in the loop, blood was analyzed for coagulation and inflammatory activation markers (TAT, β-TG, sP-selectin, SC5b-9 and PMN-elastase). Three different stent types with varying thrombogenicity were tested; statistically significant differences were found between the three stent types in measures of coagulation and platelet activation. The new Chandler-Loop model can be used as an alternative to animal and current in vitro models, especially for the determination of early events after stent implantation.
authors: Division of Congenital & Pediatric Cardiac Surgery, University Children's Hospital, Clinical Research Laboratory, Calwerstr. 7/1, 72076 Tubingen, Germany.
authors: Stefan
authors: S
authors: Sinn
authors: Torsten
authors: T
authors: Scheuermann
authors: Stephan
authors: S
authors: Deichelbohrer
authors: Gerhard
authors: G
authors: Ziemer
authors: Hans P
authors: HP
authors: Wendel
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