title: Use of pressure perturbation calorimetry to characterize the volumetric properties of proteins.
abstract: Pressure perturbation calorimetry (PPC) is a new technique that makes possible to study the volumetric changes that occur upon protein unfolding. Here, we summarize the thermodynamic foundation of the method and introduce a two-state model for the analysis of the unfolding data monitored by PPC. Several examples of data analysis illustrating potential pitfalls and solutions are discussed.
authors: Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, New York, USA.
authors: Katrina L
authors: KL
authors: Schweiker
authors: George I
authors: GI
authors: Makhatadze