title: Dynamic interaction of Y RNAs with chromatin and initiation proteins during human DNA replication.
abstract: Non-coding Y RNAs are required for the initiation of chromosomal DNA replication in mammalian cells. It is unknown how they perform this function or if they associate with a nuclear structure during DNA replication. Here, we investigate the association of Y RNAs with chromatin and their interaction with replication proteins during DNA replication in a human cell-free system. Our results show that fluorescently labelled Y RNAs associate with unreplicated euchromatin in late G1 phase cell nuclei before the initiation of DNA replication. Following initiation, Y RNAs are displaced locally from nascent and replicated DNA present in replication foci. In intact human cells, a substantial fraction of endogenous Y RNAs are associated with G1 phase nuclei, but not with G2 phase nuclei. Y RNAs interact and colocalise with the origin recognition complex (ORC), the pre-replication complex (pre-RC) protein Cdt1, and other proteins implicated in the initiation of DNA replication. These data support a molecular 'catch and release' mechanism for Y RNA function during the initiation of chromosomal DNA replication, which is consistent with Y RNAs acting as replication licensing factors.
authors: Department of Zoology, University of Cambridge, Downing Street, Cambridge CB23EJ, UK.
authors: Alice Tianbu
authors: AT
authors: Zhang
authors: Alexander R
authors: AR
authors: Langley
authors: Christo P
authors: CP
authors: Christov
authors: Eyemen
authors: E
authors: Kheir
authors: Thomas
authors: T
authors: Shafee
authors: Timothy J
authors: TJ
authors: Gardiner
authors: Torsten
authors: T
authors: Krude
references: EMBO J. 2008 Nov 19;27(22):3024-35
references: 18946490
references: J Virol. 2009 Oct;83(20):10336-46
references: 19656898
references: J Mol Med (Berl). 2004 Apr;82(4):232-9
references: 15168680
references: J Cell Sci. 1996 Feb;109 ( Pt 2):309-18
references: 8838654
references: Subcell Biochem. 2010;50:105-18
references: 20012579
references: RNA. 2009 Jul;15(7):1375-85
references: 19474146
references: Eur J Cell Biol. 1997 Oct;74(2):123-32
references: 9352217
references: J Biol Chem. 2000 May 5;275(18):13699-707
references: 10788489
references: Curr Opin Chem Biol. 2008 Dec;12(6):684-9
references: 18950732
references: EMBO J. 2007 Dec 12;26(24):5048-60
references: 18007594
references: Br J Cancer. 2008 Mar 11;98(5):981-8
references: 18283318
references: Mol Cell. 2003 Nov;12(5):1067-75
references: 14636567
references: Nat Rev Mol Cell Biol. 2004 May;5(5):367-78
references: 15122350
references: Genes Dev. 2007 Dec 1;21(23):3067-72
references: 18056422
references: J Biol Chem. 2007 Apr 6;282(14):10441-8
references: 17264083
references: Curr Opin Cell Biol. 2006 Jun;18(3):231-9
references: 16650748
references: Mol Cell Biol. 2006 Sep;26(18):6993-7004
references: 16943439
references: Nat Rev Genet. 2007 Aug;8(8):588-600
references: 17621316
references: Nat Rev Mol Cell Biol. 2005 Jun;6(6):476-86
references: 15928711
references: Gene. 1995 Mar 10;154(2):193-8
references: 7534247
references: Clin Exp Immunol. 2001 Jul;125(1):162-8
references: 11472440
references: Nat Cell Biol. 2001 Feb;3(2):107-13
references: 11175741
references: Biochim Biophys Acta. 1993 Dec 14;1216(3):395-401
references: 7505620
references: EMBO J. 1997 Jun 2;16(11):3312-9
references: 9214646
references: Nature. 1996 Aug 8;382(6591):551-5
references: 8700231
references: Mol Biol Evol. 2007 Aug;24(8):1678-89
references: 17470436
references: J Mol Biol. 1999 May 21;288(5):911-40
references: 10329189
references: Nucleic Acids Res. 2003 Mar 15;31(6):1725-34
references: 12626714
references: PLoS One. 2010 Oct 27;5(10):e13673
references: 21060685
references: Nucleic Acids Res. 2003 Jul 1;31(13):3406-15
references: 12824337
references: J Cell Sci. 1993 Nov;106 ( Pt 3):929-35
references: 7508449
references: Biochemistry. 1998 Feb 3;37(5):1336-43
references: 9477961
references: Cell. 1997 Jan 10;88(1):109-19
references: 9019396
references: Philos Trans R Soc Lond B Biol Sci. 1987 Dec 15;317(1187):483-94
references: 2894683
references: J Cell Biol. 1994 Jun;125(5):981-8
references: 8195301
references: J Cell Sci. 2008 Mar 1;121(Pt 5):590-600
references: 18252799
references: Cell. 1996 Aug 9;86(3):379-89
references: 8756720
references: J Biol Chem. 2005 Apr 1;280(13):13071-83
references: 15653697
references: J Cell Sci. 2005 Aug 1;118(Pt 15):3247-61
references: 16014376
references: J Cell Biol. 1995 Jun;129(5):1181-93
references: 7539809
references: Crit Rev Biochem Mol Biol. 2000;35(1):1-33
references: 10755664
references: Mol Cell Biol. 2010 Mar;30(6):1495-507
references: 20065034
references: Annu Rev Biochem. 2002;71:375-403
references: 12045101
references: Nucleic Acids Res. 2002 May 15;30(10):2114-23
references: 12000831
references: RNA. 2005 Jul;11(7):1084-94
references: 15928345
references: Proc Natl Acad Sci U S A. 1993 Aug 1;90(15):7250-4
references: 7688474
references: Exp Cell Res. 2005 Dec 10;311(2):240-50
references: 16226749
references: Oncogene. 2005 Apr 18;24(17):2827-43
references: 15838518
references: Chromosoma. 2000 Mar;108(8):471-84
references: 10794569
references: Annu Rev Biochem. 2002;71:333-74
references: 12045100
references: Proc Natl Acad Sci U S A. 2008 Feb 5;105(5):1692-7
references: 18234858
references: Genes Dev. 2007 Mar 1;21(5):497-518
references: 17344412
references: J Cell Sci. 2009 Aug 15;122(Pt 16):2836-45
references: 19657016
references: Theory Biosci. 2007 Aug;126(1):9-14
references: 18087752
references: Proc Natl Acad Sci U S A. 1997 Apr 1;94(7):3040-5
references: 9096342