title: Peripheral T regulatory cells and cytokines in hepatitis E infection.
abstract: This study addresses the involvement of regulatory T cells in hepatitis E (HE) infection. The study population comprised 77 acute viral HE patients, 52 recovered individuals (overall, 129 individuals with HE) and 53 healthy controls. Peripheral CD4(+)CD25(+)Foxp3(+) and CD4(+)CD25(-)Foxp3(+) frequencies by flow cytometry and HE-specific cytokines/chemokines quantitation were carried out. The median percentage of CD4(+)CD25(+)Foxp3(+) and CD4(+)CD25(-)Foxp3(+) T cells in acute patients were significantly higher compared to controls and recovered individuals. Both of the T regulatory (Treg) subset populations in overall HE were significantly elevated compared to controls. Comparisons of cytokines/chemokines revealed that the levels of IL-10 were elevated in: (a) acute viral hepatitis E (AVH-E) versus recovered individuals and controls, and (b) HE versus controls. Overall, the elevation of CD4(+)CD25(+)Foxp3(+) and CD4(+)CD25(-)Foxp3(+) frequencies and the rise in IL-10 suggest that Treg cells might be playing a pivotal role in hepatitis E virus (HEV) infection.
authors: Hepatitis Group, National Institute of Virology, 130/1, Sus Road, Pashan, Pune, Maharashtra 411021, India. anuradhastripathy@hotmail.com
authors: A S
authors: AS
authors: Tripathy
authors: R
authors: R
authors: Das
authors: S B
authors: SB
authors: Rathod
authors: Y K
authors: YK
authors: Gurav
authors: V A
authors: VA
authors: Arankalle
references: J Viral Hepat. 2008 Dec;15(12):910-6
references: 18673427
references: Vaccine. 2009 Feb 11;27(7):1032-9
references: 19095027
references: Immunol Cell Biol. 2006 Oct;84(5):467-74
references: 16869940
references: Am J Med. 1980 Jun;68(6):818-24
references: 6770682
references: J Viral Hepat. 2011 Aug;18(8):587-94
references: 20579277
references: J Virol. 2010 Sep;84(17):8790-8
references: 20573822
references: Virology. 1991 Nov;185(1):120-31
references: 1926770
references: Vaccine. 2007 May 30;25(22):4350-60
references: 17459540
references: J Virol. 2005 Mar;79(6):3322-8
references: 15731226
references: J Immunol. 2001 Aug 1;167(3):1245-53
references: 11466340
references: J Exp Med. 2007 May 14;204(5):979-85
references: 17452519
references: Clin Exp Immunol. 2006 May;144(2):188-96
references: 16634790
references: J Gastroenterol Hepatol. 2000 Jan;15(1):9-20
references: 10719741
references: Nat Immunol. 2005 Apr;6(4):345-52
references: 15785760
references: J Immunol. 2006 Jul 1;177(1):739-47
references: 16785573
references: Viral Immunol. 2007 Spring;20(1):56-65
references: 17425421
references: PLoS One. 2010 Oct 01;5(10):
references: 20957032
references: J Viral Hepat. 2007 Jun;14(6):435-45
references: 17501765
references: Intervirology. 2009;52(2):78-85
references: 19401616
references: Lancet. 1993 May 22;341(8856):1355
references: 8098491
references: Hum Immunol. 2009 May;70(5):294-9
references: 19236900
references: J Exp Med. 2003 Sep 15;198(6):889-901
references: 12975455