title: CD44 as a stem cell marker in head and neck squamous cell carcinoma.
abstract: In the recent past, evidence is increasing indicating the existence of a subpopulation of resistant tumor cells in head and neck squamous cell carcinoma (HNSCC) that cannot be eradicated by established antineoplastic treatments. These cancer stem cells (CSCs) have features of somatic stem cells such as selfrenewal, proliferation and differentiation. CD44+ cells in tumors of the head and neck are referred to as CSCs of HNSCC. Expression profiling of CD44 in 29 HNSCC tumors was performed by fluorescence microscopy. ELISA analysis was performed to detect concentration of soluble CD44 in the peripheral blood of 29 HNSCC patients and 11 healthy controls. Expression of CD44 was determined in all HNSCC tissue samples (n=29). In all samples a surface staining pattern was found. The concentration of CD44 in the peripheral blood of HNSCC patients was significantly higher compared to a healthy control group (mHNSCC =13.5 ± 0.5 ng/ ml; mCont = 9.3 ± 0.6 ng/ml; P=0.6 x 10(-12)). The role of CD44 as a marker for CSCs in HNSCC remains to be ascertained. Further experiments might reveal its role as a diagnostic and prognostic factor, and possibly as a therapeutic target.
authors: Department of Otorhinolaryngology Head and Neck Surgery, University Medical Centre Mannheim, Theodor-Kutzer-Ufer 1-3, D-68167 Mannheim, Germany.
authors: Anne
authors: A
authors: Faber
authors: Christine
authors: C
authors: Barth
authors: Karl
authors: K
authors: Hörmann
authors: Stefan
authors: S
authors: Kassner
authors: Johannes David
authors: JD
authors: Schultz
authors: Ulrich
authors: U
authors: Sommer
authors: Jens
authors: J
authors: Stern-Straeter
authors: Carsten
authors: C
authors: Thorn
authors: Ulrich Reinhart
authors: UR
authors: Goessler