title: Trace Analysis of Zn(II), Be(II), and Bi(III) by Enzyme-Catalyzed Chemiluminescence.
abstract: A novel technique for the trace analysis of metal ions Zn(II), Be(II), and Bi(III) in bulk solutions is discussed. This technique involves the generation of a chemiluminescence signal from alkaline phosphatase catalyzed hydrolysis of a phosphate derivative of 1,2-dioxetane. Zn(II) can be determined by two methods, reactivation of the alkaline phosphatase apoenzyme and inhibition of the native enzyme. Be(II) and Bi(III) can be determined quantitatively by inhibition of the native enzyme. Subppb to ppm level detection of Zn(II), Be(II), and Bi(III) has been achieved. Initial studies with mixed metals are also reported. The technique described is rapid and sensitive and can be readily applied to the microassay of heavy metal ions.
authors: Center for Advanced Materials, Departments of Chemistry and Physics, University of Massachusetts Lowell, Lowell, Massachusetts 01854, and Biotechnology Division, U.S. Army Natick RD&E Center, Natick, Massachusetts 01760.
authors: S D
authors: SD
authors: Kamtekar
authors: R
authors: R
authors: Pande
authors: M S
authors: MS
authors: Ayyagari
authors: K A
authors: KA
authors: Marx
authors: D L
authors: DL
authors: Kaplan
authors: J
authors: J
authors: Kumar
authors: S
authors: S
authors: Tripathy