title: Effect of stem cells seeded onto biomaterial on the progression of experimental chronic kidney disease.
abstract: Different routes for the administration of bone marrow-derived cells (BMDC) have been proposed to treat the progression of chronic renal failure (CRF). We investigated whether (1) the use of bovine pericardium (BP) as a scaffold for cell therapy would retard the progression of CRF and (2) the efficacy of cell therapy differently impacts distinct degrees of CRF. We used 2/3 and 5/6 models of renal mass reduction to simulate different stages of chronicity. Treatments consisted of BP seeded with either mesenchymal or mononuclear cells implanted in the parenchyma of remnant kidney. Renal function and proteinuria were measured at days 45 and 90 after cell implantation. BMDC treatment reduced glomerulosclerosis, interstitial fibrosis and lymphocytic infiltration. Immunohistochemistry showed decreased macrophage accumulation, proliferative activity and the expression of fibronectin and α-smooth muscle-actin. Our results demonstrate: (1) biomaterial combined with BMDC did retard the progression of experimental CRF; (2) cellular therapy stabilized serum creatinine (sCr), improved creatinine clearance and 1/sCr slope when administered during the less severe stages of CRF; (3) treatment with combined therapy decreased glomerulosclerosis, fibrosis and the expression of fibrogenic molecules; and (4) biomaterials seeded with BMDC can be an alternative route of cellular therapy.
authors: Department of Medicine, Laboratory of Immunology and Experimental Transplantation, Medical School of Sao Jose do Rio Preto, Av. Brigadeiro Faria Lima 5416, Sao Jose do Rio Preto, Brazil.
authors: Heloisa C
authors: HC
authors: Caldas
authors: Ida M M
authors: IM
authors: Fernandes
authors: Rosa S
authors: RS
authors: Kawasaki-Oyama
authors: Maria Alice S F
authors: MA
authors: Baptista
authors: Ana Maria G
authors: AM
authors: Plepis
authors: Virginia A
authors: VA
authors: Martins
authors: Terezila M
authors: TM
authors: Coimbra
authors: Eny M
authors: EM
authors: Goloni-Bertollo
authors: Domingo M
authors: DM
authors: Braile
authors: Mario
authors: M
authors: Abbud-Filho