title: Proliferation inhibition of cisplatin and aquaporin 5 expression in human ovarian cancer cell CAOV3.
abstract: OBJECTIVE
  The purpose of this study is to investigate the association between proliferation inhibition of cisplatin and aquaporin 5 (AQP5) expression and its regulation in ovarian carcinoma cell CAOV3.
  
  
  METHODS
  Cell growth rate was measured by MTT after CAOV3 cells were incubated with cisplatin or NF-κB inhibitor PDTC. Western blot and RT-PCR were used to detect the expression of AQP5 and NF-κB p65.
  
  
  RESULTS
  Our results showed that expression of AQP5, NF-κB in cytoplasm and karyon and IκBα in cytoplasm protein in CAOV3 cells can be induced to decrease by cisplatin with concentration-dependent manner, and there is a positive correlation between AQP5 protein and cell growth rate (r = 0.607, P < 0.05). When cells were incubated with 10 μg/ml cisplatin, AQP5, NF-κB p65, and IκBα increased rapidly at 6-12 h, but decreased at 24 h, remain on low level until to 72 h. Expression of AQP5 could be induced to decrease by PDTC, and a positive correlation between AQP5 protein expression and NF-κB p65 and IκBα (r = 0.894, 0.857; P < 0.05).
  
  
  CONCLUSIONS
  Proliferation inhibition of cisplatin is related with AQP5 expression, and NF-κB may be involved in mechanism of AQP5 regulation. AQP5 will be potential target for therapy of ovarian carcinoma.
authors: Department of Gynecology, Second Affiliated Hospital, Zhejiang University College of Medicine, 88 Jiefang Road, Hangzhou, Zhejiang, 310009, China.
authors: Jianhua
authors: J
authors: Yang
authors: Chunxiao
authors: C
authors: Yan
authors: Wei
authors: W
authors: Zheng
authors: Xuejun
authors: X
authors: Chen