title: Microsatellite markers of an important medicinal plant, Eurycoma longifolia (Simaroubaceae), for DNA profiling.
abstract: OBJECTIVE
  Microsatellite markers of an important medicinal plant, Eurycoma longifolia (Simaroubaceae), were developed for DNA profiling and genetic diversity studies.
  
  
  RESULTS
  Eighteen polymorphic microsatellite loci were developed for E. longifolia. The primers were designed from a genomic library enriched for dinucleotide (CT) repeats and screened on 32 samples from a natural population. The number of alleles detected per locus ranged from four to 16, while the observed heterozygosity ranged from 0.097 to 0.938. No significant deviation from Hardy-Weinberg equilibrium was detected in all the 18 loci, and no linkage disequilibrium was found between these loci after conservative Bonferroni correction.
  
  
  CONCLUSIONS
  The 18 microsatellite markers of E. longifolia are highly polymorphic and informative. These markers would serve as an important tool for DNA profiling and genetic diversity studies.
authors: Genetic Laboratory, Forest Research Institute Malaysia 52109 Kepong, Selangor, Malaysia. leehong@frim.gov.my
authors: Lee Hong
authors: LH
authors: Tnah
authors: Chai Ting
authors: CT
authors: Lee
authors: Soon Leong
authors: SL
authors: Lee
authors: Kevin Kit Siong
authors: KK
authors: Ng
authors: Chin Hong
authors: CH
authors: Ng
authors: Siaw San
authors: SS
authors: Hwang