title: Haematopoietic stem cell differentiation promotes the release of prominin-1/CD133-containing membrane vesicles--a role of the endocytic-exocytic pathway.
abstract: The differentiation of stem cells is a fundamental process in cell biology and understanding its mechanism might open a new avenue for therapeutic strategies. Using an ex vivo co-culture system consisting of human primary haematopoietic stem and progenitor cells growing on multipotent mesenchymal stromal cells as a feeder cell layer, we describe here the exosome-mediated release of small membrane vesicles containing the stem and cancer stem cell marker prominin-1 (CD133) during haematopoietic cell differentiation. Surprisingly, this contrasts with the budding mechanism underlying the release of this cholesterol-binding protein from plasma membrane protrusions of neural progenitors. Nevertheless, in both progenitor cell types, protein-lipid assemblies might be the essential structural determinant in the release process of prominin-1. Collectively, these data support the concept that prominin-1-containing lipid rafts may host key determinants necessary to maintain stem cell properties and their quantitative reduction or loss may result in cellular differentiation.
authors: Tissue Engineering Laboratories (BIOTEC), Technische Universität Dresden, Dresden, Germany.
authors: Nicola
authors: N
authors: Bauer
authors: Michaela
authors: M
authors: Wilsch-Bräuninger
authors: Jana
authors: J
authors: Karbanová
authors: Ana-Violeta
authors: AV
authors: Fonseca
authors: Doreen
authors: D
authors: Strauss
authors: Daniel
authors: D
authors: Freund
authors: Christoph
authors: C
authors: Thiele
authors: Wieland B
authors: WB
authors: Huttner
authors: Martin
authors: M
authors: Bornhäuser
authors: Denis
authors: D
authors: Corbeil
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