title: Variation in gymnemic acid content and non-destructive harvesting of Gymnema sylvestre (Gudmar).
abstract: BACKGROUND
  Madhunashini (Gymnema sylvestre R. Br.) commonly known as 'Gudmar' in Hindi is an important medicinal climber and extensively used in almost all Indian System of Medicine as a remedy for diabetes, rheumatism, cough, ulcer, jaundice, dyspepsia, constipation, eyes pain and also in snakebite. In India, it is found growing in Andhra Pradesh, Bihar, Chhattisgarh, Karnataka, Kerala, Madhya Pradesh, Maharastra, Orissa, Tamil Nadu, Uttar Pradesh and West Bengal. The major phytoconstituents are gymnemic acids, gudmarin and saponins.
  
  
  METHODS
  In the present study, Gymnema germplasm collected from various regions of Madhya Pradesh was evaluated on the basis of their morphological characteristics and gymnemic acid content. Gymnemic acid content in the leaves was estimated by HPLC. We have also standardized the non-destructive harvesting practices of Gudmar. Selective harvesting was done without harming the main plant. Only mature leaves (60%) were hand plucked in the month of October. Second harvest was done in the month of June.
  
  
  RESULTS
  Data revealed that gymnemic acid content varied between 0.96% ± 0.03 (Seoni) to 1.58% ±0.03 (Amarkantak). It was also observed that the leaves left at the time of 1(st) harvest during October matured in June at the time of 2(nd) harvest.
  
  
  CONCLUSIONS
  Non destructive harvesting practice did not have any negative impact on overall development of the plant. It is evident that there is wide variation in the morphological characteristics and gymnemic acid content in G. sylvestre collected from various locations, which can be exploited for further crop improvement programmes.
authors: Tropical Forest Research Institute (Indian Council of Forestry Research and Education), P.O. RFRC, Mandla Road, Jabalpur - 482 021,M.P., India.
authors: Ashok Kumar
authors: AK
authors: Pandey
authors: Swati
authors: S
authors: Yadav
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