title: Identification of Ski as a target for Aurora A kinase.
abstract: Ski is a negative regulator of the transforming growth factor-β and other signalling pathways. The absence of SKI in mouse fibroblasts leads to chromosome segregation defects and genomic instability, suggesting a role for Ski during mitosis. At this stage, Ski is phosphorylated but to date little is known about the kinases involved in this process. Here, we show that Aurora A kinase is able to phosphorylate Ski in vitro. In vivo, Aurora A and Ski co-localized at the centrosomes and co-immunoprecipitated. Conversely, a C-terminal truncation mutant of Ski (SkiΔ491-728) lacking a coiled-coil domain, displayed decreased centrosomal localization. This mutant no longer co-immunoprecipitated with Aurora-A in vivo, but was still phosphorylated in vitro, indicating that the Ski-Aurora A interaction takes place at the centrosomes. These data identify Ski as a novel target of Aurora A and contribute to an understanding of the role of these proteins in the mitotic process.
authors: Programa de Genética Humana, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile.
authors: Jocelyn
authors: J
authors: Mosquera
authors: Ricardo
authors: R
authors: Armisen
authors: Hongling
authors: H
authors: Zhao
authors: Diego A
authors: DA
authors: Rojas
authors: Edio
authors: E
authors: Maldonado
authors: Julio C
authors: JC
authors: Tapia
authors: Alicia
authors: A
authors: Colombo
authors: Michael J
authors: MJ
authors: Hayman
authors: Katherine
authors: K
authors: Marcelain
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