title: Structure of type 3Gn coaggregation receptor polysaccharide from Streptococcus cristatus LS4.
abstract: The presence of a novel coaggregation receptor polysaccharide (RPS) on the dental plaque isolate Streptococcus cristatus LS4 was suggested by this strain's antigenic and coaggregation properties. Examination of RPS isolated from strain LS4 by a combination of 2-dimensional and pseudo 3-dimensional single quantum heteronuclear NMR methods that included detection of (13)C chemical shifts at high resolution revealed the following repeat unit structure: →6)-β-d-Galf-(1→6)-β-d-GalpNAc-(1→3)-α-d-Galp-(1→P→6)-α-d-Galp-(1→3)-β-L-Rhap-(1→4)-β-d-Glcp-(1→. The identification of this polysaccharide as RPS3Gn, a new structural type, was established by the α-d-Galp-containing epitope of RPS serotype 3 and Gn recognition motif (i.e., β-d-GalpNAc (1→3)-α-d-Galp) for coaggregation with other bacteria.
authors: Oral Infection and Immunity Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, United States.
authors: Jinghua
authors: J
authors: Yang
authors: John O
authors: JO
authors: Cisar
authors: C Allen
authors: CA
authors: Bush
references: Infect Immun. 1979 Jun;24(3):742-52
references: 468376
references: Carbohydr Res. 1989 Aug 15;191(2):279-93
references: 2582463
references: Glycobiology. 1995 Oct;5(7):655-62
references: 8608267
references: J Biol Chem. 2008 May 2;283(18):12654-64
references: 18303023
references: Glycobiology. 2005 Feb;15(2):139-51
references: 15371346
references: J Clin Microbiol. 2005 Dec;43(12):6073-85
references: 16333101
references: Biochemistry. 1991 Sep 3;30(35):8568-77
references: 1888724
references: J Biol Chem. 2010 Jul 30;285(31):24217-27
references: 20507989
references: BMC Oral Health. 2006 Jun 15;6 Suppl 1:S12
references: 16934113
references: Mol Microbiol. 2005 Oct;58(1):244-56
references: 16164562
references: J Bacteriol. 2006 Jun;188(11):4125-30
references: 16707704
references: Biochemistry. 1991 Jul 2;30(26):6528-40
references: 2054351
references: J Bacteriol. 2009 Mar;191(6):1891-900
references: 19151140
references: Oral Microbiol Immunol. 2002 Aug;17(4):257-62
references: 12121477
references: J Bacteriol. 2007 Nov;189(21):7841-55
references: 17766424
references: Biochemistry. 1990 Jan 9;29(1):234-48
references: 2157479
references: BMC Biol. 2009 Jan 26;7:3
references: 19171050
references: Adv Dent Res. 1997 Apr;11(1):168-75
references: 9524453
references: Infect Immun. 1997 Dec;65(12):5035-41
references: 9393793
references: Glycobiology. 1994 Apr;4(2):183-92
references: 8054717
references: J Bacteriol. 2003 Sep;185(18):5419-30
references: 12949094