title: Alpha-tocopherol prevents intrauterine undernutrition-induced oligonephronia in rats.
abstract: The role of α-tocopherol during nephrogenesis was investigated in rats subjected to maternal undernutrition, which reduces the number of nephrons. α-tocopherol (350 mg/kg, p.o.) was administered daily to well-nourished or malnourished Wistar dams during pregnancy, or to prenatal undernourished rats during lactation. The kidneys of 1- and 25-day-old offspring were removed to evaluate expression of angiotensin II (Ang II) and to correlate this with expression of proliferating cell nuclear antigen, α-smooth muscle actin, fibronectin and vimentin in the glomeruli and tubulointerstitial space. One-day-old prenatally undernourished rats had reduced expression of Ang II and of kidney development markers, and presented with an enlarged nephrogenic zone. Maternal administration of α-tocopherol restored the features of normal kidney development in undernourished rats. Twenty-five-day-old prenatally undernourished progeny had fewer glomeruli than the control group. Conversely, animals from mothers that received α-tocopherol during lactation presented with the same number of glomeruli and the same glomerular morphometrical profile as the control group. Analyzing the levels of thiobarbituric acid reactive substances in the liver in conjunction with kidney development markers, it is plausible that α-tocopherol had antioxidant and non-antioxidant actions. This study provides evidence that α-tocopherol treatment restored Ang II expression, and subsequently restored renal structural development.
authors: Departamento de Fisiologia e Farmacologia, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, Av. Prof. Moraes Rego, s/n, Cidade Universitária 50670-901, Recife, PE, Brazil.
authors: Leucio D
authors: LD
authors: Vieira-Filho
authors: Edjair Vicente
authors: EV
authors: Cabral
authors: Felipe T J
authors: FT
authors: Santos
authors: Terezila M
authors: TM
authors: Coimbra
authors: Ana D O
authors: AD
authors: Paixão
references: Methods Enzymol. 1978;52:302-10
references: 672633
references: Kidney Int. 1999 Sep;56(3):1072-7
references: 10469376
references: Mech Dev. 2010 Jan-Feb;127(1-2):21-7
references: 19961928
references: Br J Nutr. 2005 Feb;93(2):153-74
references: 15788108
references: Kidney Int. 2002 Apr;61(4):1231-42
references: 11918729
references: Am J Clin Nutr. 2006 Dec;84(6):1442-8
references: 17158428
references: BMJ. 1990 Aug 4;301(6746):259-62
references: 2390618
references: Basic Clin Pharmacol Toxicol. 2011 Feb;108(2):115-21
references: 20735790
references: Reprod Biol Endocrinol. 2010 Jul 12;8:86
references: 20624307
references: J Physiol. 1977 Nov;272(3):573-89
references: 592203
references: Pediatr Res. 2001 Apr;49(4):460-7
references: 11264427
references: Am J Physiol Renal Physiol. 2007 Aug;293(2):F506-10
references: 17442728
references: Am J Physiol Renal Physiol. 2002 Jul;283(1):F202-6
references: 12060603
references: Pediatr Nephrol. 1997 Dec;11(6):719-23
references: 9438651
references: J Cardiovasc Pharmacol. 2003 Aug;42(2):211-7
references: 12883324
references: Anat Embryol (Berl). 1998 Feb;197(2):143-53
references: 9497157
references: Pediatr Nephrol. 2004 Nov;19(11):1212-8
references: 15338391
references: Biol Neonate. 2001;80(3):239-46
references: 11585990
references: Am J Physiol Regul Integr Comp Physiol. 2005 Jan;288(1):R80-4
references: 15458966
references: Z Wiss Mikrosk. 1961 Dec;65:70-82
references: 13889833
references: Clin Exp Pharmacol Physiol. 2009 Dec;36(12):1157-63
references: 19473191
references: Am J Physiol Renal Physiol. 2002 Oct;283(4):F727-33
references: 12217864
references: J Am Soc Nephrol. 2005 Jan;16(1):189-94
references: 15548563
references: Life Sci. 1999;64(11):965-74
references: 10201645
references: Acta Histochem. 2005;107(4):291-300
references: 16181665
references: Exp Physiol. 2006 May;91(3):611-9
references: 16513822
references: J Cell Biol. 1987 Oct;105(4):1549-54
references: 2889739
references: Am J Physiol Renal Physiol. 2010 Mar;298(3):F807-17
references: 20032120
references: Kidney Int. 2008 Nov;74(10):1287-93
references: 18650792
references: Arch Biochem Biophys. 2011 Jan 1;505(1):91-7
references: 20887709
references: Pediatr Nephrol. 2010 Jun;25(6):1063-72
references: 20130919
references: Pediatr Nephrol. 1994 Apr;8(2):175-80
references: 8018495
references: Cardiovasc Res. 2002 Oct;56(1):145-53
references: 12237175
references: Lab Invest. 1963 Feb;12:131-55
references: 13999512
references: Eur J Pharmacol. 2008 Jun 24;588(1):114-23
references: 18495111
references: Kidney Int. 2000 Jan;57(1):167-82
references: 10620198
references: Hypertension. 1992 Feb;19(2 Suppl):II168-75
references: 1735573
references: J Biol Chem. 2000 Mar 31;275(13):9527-33
references: 10734101
references: Am J Obstet Gynecol. 2008 Sep;199(3):252.e1-7
references: 18639218
references: Hypertension. 2007 Jun;49(6):1429-35
references: 17404180
references: J Am Soc Nephrol. 2006 Apr;17(4):1005-14
references: 16495379
references: Free Radic Biol Med. 2007 Jul 1;43(1):16-21
references: 17561089
references: Am J Physiol Renal Physiol. 2003 Aug;285(2):F199-207
references: 12657564
references: Comp Biochem Physiol A Physiol. 1995 Mar;110(3):223-8
references: 7712066
references: Am J Physiol. 1995 Jul;269(1 Pt 2):F110-5
references: 7631824
references: Biol Chem. 2004 Jul;385(7):585-91
references: 15318806