title: 1-o-octadecyl-2-o-methyl-glycerophosphoscholine inhibits the adhesion of ras-transformed fibroblasts to human endothelial-cells.
abstract: The adhesion of tumor cells to the endothelium is a key step in haematogenic metastasis. Because alkyllysophospholipids decrease tumor metastasis, we investigated the effect of 1-O-octadecyl-2-O-methyl-glycerophosphocholine (ET18-OCH3) on the adhesion of two highly metastatic ras-transformed 10T1/2 cell lines, CIRAS-2 and CIRAS-3, to confluent endothelial cells. The order of adhesion to the endothelial cells was CIRAS-2 > 10T1/2 > CIRAS-3. RGD peptides were unable to inhibit the binding of the cells suggesting that adhesion was not mediated by fibronectin or vitronectin. Treatment of the cell lines with 20 mu M ET18-OCH3 for 24 h decreased the adhesion of CIRAS-2, CIRAS-3 and 10T1/2 to the endothelial cells by 50, 43 and 36% respectively. Thus interference in the adhesion of tumor cells to the endothelium could contribute to decreased metastasis.
authors: UNIV MANITOBA,DEPT BIOCHEM & MOLEC BIOL,WINNIPEG,MB R3E 0W3,CANADA.
authors: P
authors: P
authors: Samadder
authors: X
authors: X
authors: Lu
authors: G
authors: G
authors: Arthur