title: Paracoccidioides brasilinsis-induced migration of dendritic cells and subsequent T-cell activation in the lung-draining lymph nodes.
abstract: Paracoccidioidomycosis is a mycotic disease caused by a dimorphic fungus, Paracoccidioides brasiliensis (Pb), that starts with inhalation of the fungus; thus, lung cells such as DC are part of the first line of defense against this microorganism. Migration of DC to the lymph nodes is the first step in initiating T cell responses. The mechanisms involved in resistance to Pb infection are poorly understood, but it is likely that DC play a pivotal role in the induction of effector T cells that control Pb infection. In this study, we showed that after Pb Infection, an important modification of lung DC receptor expression occurred. We observed an increased expression of CCR7 and CD103 on lung DC after infection, as well as MHC-II. After Pb infection, bone marrow-derived DC as well lung DC, migrate to lymph nodes. Migration of lung DC could represent an important mechanism of pathogenesis during PCM infection. In resume our data showed that Pb induced DC migration. Furthermore, we demonstrated that bone marrow-derived DC stimulated by Pb migrate to the lymph nodes and activate a T helper (Th) response. To the best of our knowledge, this is the first reported data showing that Pb induces migration of DC and activate a T helper (Th) response.
authors: Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Brasil.
authors: Suelen
authors: S
authors: Silvana dos Santos
authors: Karen Spadari
authors: KS
authors: Ferreira
authors: Sandro Rogerio
authors: SR
authors: Almeida
references: Mycopathologia. 2008 Apr-May;165(4-5):209-21
references: 18777630
references: Infect Immun. 2006 Jul;74(7):3817-24
references: 16790753
references: Immunity. 2004 Aug;21(2):279-88
references: 15308107
references: J Virol. 2009 Jul;83(14):7235-43
references: 19420085
references: Braz J Med Biol Res. 1998 May;31(5):615-23
references: 9698765
references: J Exp Med. 2001 May 21;193(10):1135-47
references: 11369785
references: Infect Immun. 2008 Jun;76(6):2362-7
references: 18391001
references: Immunol Lett. 2004 Jun 15;94(1-2):107-14
references: 15234542
references: Eur J Immunol. 2010 Jan;40(1):153-61
references: 19830735
references: Annu Rev Immunol. 2008;26:293-316
references: 18045026
references: J Immunol. 2000 Feb 15;164(4):2021-7
references: 10657654
references: J Virol. 2010 Oct;84(19):10191-9
references: 20660207
references: J Infect Dis. 2007 Oct 1;196(7):1108-15
references: 17763336
references: Nat Immunol. 2006 Jul;7(7):681-5
references: 16785881
references: J Exp Med. 1992 Dec 1;176(6):1693-702
references: 1460426
references: J Immunol. 2004 Jul 1;173(1):494-506
references: 15210810
references: Mem Inst Oswaldo Cruz. 2009 May;104(3):513-21
references: 19547881
references: Curr Opin Immunol. 2009 Feb;21(1):105-10
references: 19342210
references: J Immunol. 2002 Feb 1;168(3):1362-71
references: 11801677
references: Mycopathologia. 2008 Apr-May;165(4-5):197-207
references: 18777629
references: Cytokine. 2001 Feb 21;13(4):248-52
references: 11237434
references: Infect Immun. 2001 Feb;69(2):1127-33
references: 11160010
references: Infect Immun. 2009 Sep;77(9):3749-58
references: 19564388
references: Infect Immun. 1991 Feb;59(2):521-8
references: 1898907
references: Clin Immunol. 2005 Jan;114(1):86-94
references: 15596413
references: J Leukoc Biol. 2007 Jul;82(1):33-43
references: 17440035
references: J Exp Med. 2006 Jul 10;203(7):1805-15
references: 16818672