title: Structural components and architectures of RNA exosomes.
abstract: A large body of structural work conducted over the past ten years has elucidated mechanistic details related to 3' to 5' processing and decay of RNA substrates by the RNA exosome. This chapter will focus on the structural organization of eukaryotic exosomes and their evolutionary cousins in bacteria and archaea with an emphasis on mechanistic details related to substrate recognition and to 3' to 5' phosphorolytic exoribonucleolytic activities of bacterial and archaeal exosomes as well as the hydrolytic exoribonucleolytic and endoribonucleolytic activities of eukaryotic exosomes. These points will be addressed in large part through presentation of crystal structures ofphosphorolytic enzymes such as bacterial RNase PH, PNPase and archaeal exosomes and crystal structures ofthe eukaryotic exosome and exosome sub-complexes in addition to standalone structures of proteins that catalyze activities associated with the eukaryotic RNA exosome, namely Rrp44, Rrp6 and their bacterial counterparts.
authors: Structural Biology Program, Sloan-Kettering Institute, New York, New York, USA.
authors: Kurt
authors: K
authors: Januszyk
authors: Christopher D
authors: CD
authors: Lima
references: Biochem Soc Trans. 2002 Apr;30(2):150-5
references: 12035760
references: Genome Res. 2001 Feb;11(2):240-52
references: 11157787
references: Mol Cell. 2005 Nov 11;20(3):473-81
references: 16285928
references: J Biol Chem. 2006 Mar 10;281(10):6751-9
references: 16407194
references: Proc Natl Acad Sci U S A. 2007 Oct 23;104(43):16844-9
references: 17942686
references: Biochim Biophys Acta. 2008 Sep;1779(9):558-65
references: 18313413
references: Nat Struct Mol Biol. 2005 Jul;12(7):575-81
references: 15951817
references: J Biol Chem. 2010 Feb 5;285(6):3540-3547
references: 19955569
references: J Biol Chem. 2004 Apr 16;279(16):16471-8
references: 14734548
references: Mol Biochem Parasitol. 2008 May;159(1):24-9
references: 18279979
references: EMBO J. 2006 Nov 1;25(21):5117-25
references: 17053788
references: Nature. 2006 Sep 7;443(7107):110-4
references: 16957732
references: Cell. 2006 Dec 15;127(6):1223-37
references: 17174896
references: Cell. 1997 Jan 24;88(2):235-42
references: 9008164
references: RNA. 2008 Nov;14(11):2361-71
references: 18812438
references: Structure. 2000 Nov 15;8(11):1215-26
references: 11080643
references: Cell. 2007 May 18;129(4):651-3
references: 17512398
references: Mol Cell. 2005 Nov 11;20(3):461-71
references: 16285927
references: Proc Natl Acad Sci U S A. 2006 Aug 8;103(32):11898-903
references: 16882719
references: J Bacteriol. 2005 Nov;187(21):7214-21
references: 16237005
references: Mol Cell. 2005 Jan 21;17(2):313-8
references: 15664199
references: Protein Sci. 1992 Nov;1(11):1494-507
references: 1303768
references: Cell. 2001 Nov 16;107(4):451-64
references: 11719186
references: Cell. 2009 Oct 30;139(3):547-59
references: 19879841
references: EMBO Rep. 2007 May;8(5):470-6
references: 17380186
references: Protein Sci. 1992 Nov;1(11):1508-21
references: 1303769
references: J Biol Chem. 2003 Aug 22;278(34):32397-404
references: 12746447
references: Proc Natl Acad Sci U S A. 1993 Jul 15;90(14):6498-502
references: 8341661
references: Nat Struct Mol Biol. 2007 Jan;14(1):15-22
references: 17173052
references: EMBO Rep. 2003 Sep;4(9):889-93
references: 12947419
references: Cell. 1997 Nov 14;91(4):457-66
references: 9390555
references: RNA. 2008 Nov;14(11):2417-29
references: 18824515
references: EMBO Rep. 2006 Jun;7(6):605-10
references: 16729021
references: EMBO Rep. 2007 Jan;8(1):63-9
references: 17159918
references: FEBS J. 2008 Jun;275(11):2712-26
references: 18422648
references: J Mol Biol. 2002 Aug 16;321(3):397-409
references: 12162954
references: Structure. 2005 Jul;13(7):973-84
references: 16004870
references: Biochim Biophys Acta. 2008 Apr;1779(4):239-46
references: 18211833
references: J Biol Chem. 2002 Jun 14;277(24):21624-9
references: 11948193
references: Mol Cell. 2008 Mar 28;29(6):717-28
references: 18374646
references: Nucleic Acids Res. 2008 Dec;36(21):6645-55
references: 18940861
references: J Mol Biol. 2009 May 29;389(1):17-33
references: 19327365
references: Protein Sci. 2004 Mar;13(3):668-77
references: 14767080
references: J Biol Chem. 2008 May 16;283(20):14120-31
references: 18353775
references: Genome Res. 2004 Jul;14(7):1315-23
references: 15231747
references: Prog Nucleic Acid Res Mol Biol. 1983;28:101-42
references: 6348874
references: RNA. 2003 Sep;9(9):1098-107
references: 12923258
references: J Bacteriol. 1999 Jan;181(1):24-33
references: 9864308
references: J Am Chem Soc. 2008 Aug 20;130(33):10955-62
references: 18662000
references: Nature. 1985 Feb 28-Mar 6;313(6005):762-6
references: 3883192
references: Mol Cell. 2007 Jul 20;27(2):324-31
references: 17643380
references: J Mol Biol. 2004 Jul 30;341(1):37-54
references: 15312761
references: Mol Cell. 2009 Feb 13;33(3):400-9
references: 19217413
references: Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 May 1;61(Pt 5):463-8
references: 16511069
references: EMBO J. 1991 Jan;10(1):25-33
references: 1989886