title: Moving and fusion of the pancreatic buds in the rat embryos during the embryonic period (carnegie stages 13-17) by a three-dimensional computer-assisted reconstruction.
abstract: OBJECTIVE
  The purpose of the present study was to illustrate the modality of rotation of ventral and dorsal pancreatic buds by three-dimensional (3D) reconstructions in the rat embryos, during the Carnegie stages 13-17.
  
  
  METHODS
  Serial sections of thirty rat embryos stages 13-17, were observed. The embryos were fixed in Bouin's solution, dehydrated, and paraffin embedded. The sections, 7 μm thick, were cut in longitudinal or transverse planes and were stained alternately by hematoxylin-eosin or Heindenhain' azan. The images were digitalized by Canon Camera 350 EOS D. The 3D reconstruction was performed by computer using Cell Image Analyser software.
  
  
  RESULTS
  The two pancreatic buds ventral and dorsal, were clearly identified at stage 13, in anterior and posterior position, respectively, in relation to the duodenum. In stage 15, the duodenum started its rotation of 90° clockwise. The ventral bud moved 90° from the midline to the right. In stage 16, the ventral pancreas continued its rotation until 180° in posterior position behind the duodenum. In stage 17, the two pancreatic buds were related closely to the ventral part of the portal vein. The two buds began to merge. The anterior face of the pancreas's head was arising from the dorsal pancreatic bud. The rest of the head including the omental tuberosity and the uncinate process emanated from the ventral pancreatic bud.
  
  
  CONCLUSIONS
  The use of 3D reconstruction of the pancreas of rat embryos illustrates the modality of the two pancreatic buds rotation and fusion. This method explains the final position of the pancreas.
authors: Laboratory of Experimental Anatomy Faculty of Medicine Montpellier-Nîmes, University Montpellier I, Avenue Kennedy, 30908, Nimes, France. marlene.villon@univ-montp1.fr
authors: Guilhem
authors: G
authors: Godlewski
authors: Jacques
authors: J
authors: Gaubert
authors: Renée
authors: R
authors: Cristol-Gaubert
authors: Maïada
authors: M
authors: Radi
authors: Volker
authors: V
authors: Baecker
authors: Pierre
authors: P
authors: Travo
authors: Michel
authors: M
authors: Prudhomme
authors: Dominique
authors: D
authors: Prat-Pradal
references: J Chir (Paris). 1977;114(3):185-92
references: 925106
references: J Anat. 1960 Apr;94:231-40
references: 14404265
references: Development. 1995 Jun;121(6):1569-80
references: 7600975
references: Anat Clin. 1984;5(4):275-83
references: 6372837
references: C R Acad Hebd Seances Acad Sci D. 1976 Oct 27;283(10):1213-6
references: 827358
references: Surg Radiol Anat. 2009 Jan;31(1):31-3
references: 18813869
references: Mech Dev. 2003 Jan;120(1):59-64
references: 12490296
references: Yonsei Med J. 1992 Jun;33(2):104-8
references: 1413888
references: Anat Rec. 1951 Oct;111(2):255-70
references: 14894836
references: J Anat. 1930 Oct;65(Pt 1):1-7
references: 17104298