title: Fluorescent gallium and indium bis(thiosemicarbazonates) and their radiolabelled analogues: synthesis, structures and cellular confocal fluorescence imaging investigations.
abstract: New fluorescent and biocompatible aromatic Ga(III)- and In(III)-bis(thiosemicarbazonato) complexes for dual mode optical and PET or SPECT molecular imaging have been synthesised via a synthetic method based on transmetallation reactions from Zn(II) precursors. Complexes have been fully characterised in the solid state by single crystal X-ray diffraction and in solution by spectroscopic methods (UV/Vis, fluorescence, (1)H and (13)C{(1)H} NMR). The bis(thiosemicarbazones) radiolabelled rapidly in high yields under mild conditions with (111)In (a gamma and Auger emitter for SPECT imaging and radiotherapy with t(1/2) = 2.8 d) and (68)Ga (a generator-available positron emitter for PET imaging with t(1/2) = 68 min). Cytotoxicity and biolocalisation studies using confocal fluorescence imaging and fluorescence lifetime imaging (FLIM) techniques have been used to study their in vitro activities and stabilities in HeLa and PC-3 cells to ascertain their suitability as synthetic scaffolds for future multimodality molecular imaging in cancer diagnosis and therapy. The observation that the indium complexes show certain nuclear uptake could be of relevance towards developing (111)In therapeutic agents based on Auger electron emission to induce DNA damage.
authors: Chemistry Department, University of Bath, Bath, UKBA2 7AY, UK.
authors: Rory L
authors: RL
authors: Arrowsmith
authors: Philip A
authors: PA
authors: Waghorn
authors: Michael W
authors: MW
authors: Jones
authors: Andreas
authors: A
authors: Bauman
authors: Simon K
authors: SK
authors: Brayshaw
authors: Zhiyuan
authors: Z
authors: Hu
authors: Gabriele
authors: G
authors: Kociok-Köhn
authors: Thomas L
authors: TL
authors: Mindt
authors: Rex M
authors: RM
authors: Tyrrell
authors: Stanley W
authors: SW
authors: Botchway
authors: Jonathan R
authors: JR
authors: Dilworth
authors: Sofia I
authors: SI
authors: Pascu