title: Ginsenoside-Rg1 protects podocytes from complement mediated injury.
abstract: OBJECTIVE
  Podocytes injury mediated by complement complex C5b-9 is the main feature of membranous nephropathy (MN). Little work has been done to prove that ginsenoside-Rg1 could inhibit this process. Our study aims to investigate the efficacy of ginsenoside-Rg1 in protecting the podocyte from complement mediated injury.
  
  
  METHODS
  We chose sublethal C5b-9 induced podocyte injury as the model of MN in vitro. Ginsenoside-Rg1 was given as an intervention. Morphological changes were observed by electron microscope and fluorescence microscope. The production of reactive oxygen species (ROS) was detected by flow cytometry. The expression of the mitogen activated protein kinase (MAPK) including JNK, ERK and P38 was detected by western-blot technique.
  
  
  RESULTS
  Ginsenoside-Rg1 could protect foot processes of podocytes, suppress the damage of F-actin, decrease the production of ROS, and inhibit the activation of P38 kinase pathway.
  
  
  CONCLUSIONS
  These results suggest that ginsenoside-Rg1 could protect podocyte from sMAC-induced injury partly because of its antioxidant property and inhibit the activation of P38 kinase pathway.
authors: Department of Nephrology, West China Hospital of Sichuan University, Chengdu City, Sichuan Province, China.
authors: Ming-Hua
authors: MH
authors: Zhang
authors: Jun-Ming
authors: JM
authors: Fan
authors: Xi-Sheng
authors: XS
authors: Xie
authors: Yue-Yi
authors: YY
authors: Deng
authors: Yi-Pin
authors: YP
authors: Chen
authors: Rong
authors: R
authors: Zhen
authors: Jing
authors: J
authors: Li
authors: Yuan
authors: Y
authors: Cheng
authors: Ji
authors: J
authors: Wen