title: A novel procedure for protein extraction from formalin-fixed paraffin-embedded tissues.
abstract: Most of the archived pathological specimens in hospitals are kept as formalin-fixed paraffin-embedded tissues (FFPE) for long-term preservation. Up to now, these samples are only used for immunohistochemistry in a clinical routine as it is difficult to recover intact protein from these FFPE tissues. Here, we report a novel, short time-consuming and cost-effective method to extract full-length, non-degraded proteins from FFPE tissues. This procedure is combined with an effective and non-toxic deparaffinisation process and an extraction method based on antigen-retrieval, high concentration of SDS and high temperature. We have obtained enough intact protein to be detected by Western blotting analysis. This technique will allow utilising these stored FFPE tissues in several applications for protein analysis helping to advance the translational studies in cancer and other diseases.
authors: Translational Cancer Research Group, Proteo-Red-ISCIII, Instituto de Investigación Biomédica A Coruña, Coruña, Spain.
authors: Teresa
authors: T
authors: Rodríguez-Rigueiro
authors: Manuel
authors: M
authors: Valladares-Ayerbes
authors: Mar
authors: M
authors: Haz-Conde
authors: Moisés
authors: M
authors: Blanco
authors: Guadalupe
authors: G
authors: Aparicio
authors: Patricia
authors: P
authors: Fernández-Puente
authors: Francisco J
authors: FJ
authors: Blanco
authors: M José
authors: MJ
authors: Lorenzo
authors: Luis A
authors: LA
authors: Aparicio
authors: Angélica
authors: A
authors: Figueroa