title: LEUNIG and SEUSS co-repressors regulate miR172 expression in Arabidopsis flowers.
abstract: Central to the ABCE model of flower development is the antagonistic interaction between class A and class C genes. The molecular mechanisms underlying the A-C antagonism are not completely understood. In Arabidopsis thaliana, miR172 is expressed in the inner floral whorls where it downregulates the class A gene APETALA 2 (AP2). However, what controls this predominantly inner whorl-specific expression of miR172 is not known. We show that the LEUNIG (LUG) and SEUSS (SEU) co-repressors repress miR172 expression in the outer whorls of A. thaliana flowers. The recruitment of LUG/SEU to the miR172 promoters is dependent on AP2, suggesting that AP2 represses the expression of its cognate microRNA. Our study provides new insights into the molecular mechanisms underlying the A-C antagonism and shed light on the transcriptional regulation of miR172 during flower development.
authors: Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA.
authors: Boyana
authors: B
authors: Grigorova
authors: Chloe
authors: C
authors: Mara
authors: Courtney
authors: C
authors: Hollender
authors: Paja
authors: P
authors: Sijacic
authors: Xuemei
authors: X
authors: Chen
authors: Zhongchi
authors: Z
authors: Liu
references: Science. 2004 Mar 26;303(5666):2022-5
references: 12893888
references: Cell. 2001 Jun 15;105(6):793-803
references: 11440721
references: Plant J. 2004 Nov;40(3):428-38
references: 15469500
references: Development. 1995 Apr;121(4):975-91
references: 7743940
references: Genome Biol. 2009;10(11):R122
references: 19889219
references: Development. 2002 Jan;129(1):253-63
references: 11782418
references: Nat Genet. 2007 Dec;39(12):1517-21
references: 18026103
references: Development. 1991 May;112(1):1-20
references: 1685111
references: Plant Cell. 2006 Jun;18(6):1373-82
references: 16679456
references: Development. 2006 Aug;133(16):3159-66
references: 16854969
references: Plant J. 2009 Nov;60(4):626-37
references: 19656343
references: Nat Protoc. 2008;3(6):1077-84
references: 18536652
references: Plant Physiol. 2008 Mar;146(3):1165-81
references: 18184731
references: Proc Natl Acad Sci U S A. 2005 Jun 28;102(26):9412-7
references: 15958531
references: Science. 2001 Oct 26;294(5543):862-4
references: 11679672
references: Trends Plant Sci. 2008 Mar;13(3):137-44
references: 18314376
references: Proc Natl Acad Sci U S A. 2000 Nov 7;97(23):12902-7
references: 11058164
references: Nature. 2001 Jan 25;409(6819):469-71
references: 11206529
references: Planta. 2006 Aug;224(3):612-21
references: 16523347
references: Development. 2010 Nov;137(21):3633-42
references: 20876650
references: Plant Cell. 2003 Nov;15(11):2730-41
references: 14555699
references: Cell. 1994 Jan 14;76(1):131-43
references: 7506995
references: Proc Natl Acad Sci U S A. 2004 Aug 3;101(31):11494-9
references: 15277686
references: Science. 2001 Oct 26;294(5543):858-62
references: 11679671
references: J Vis Exp. 2010 Jun 12;(40):
references: 20567209
references: Plant Cell. 2007 Sep;19(9):2736-48
references: 17890372
references: Cell. 1991 Jun 14;65(6):991-1002
references: 1675158
references: Methods. 2001 Dec;25(4):402-8
references: 11846609
references: Nature. 1991 Sep 5;353(6339):31-7
references: 1715520
references: Planta. 1997;201(2):179-88
references: 9084216
references: Nat Protoc. 2008;3(4):698-709
references: 18388953
references: Nature. 1992 Nov 19;360(6401):273-7
references: 1359429
references: Plant Physiol. 2008 Jun;147(2):672-81
references: 18390806
references: Development. 2009 Sep;136(17):2873-81
references: 19666819
references: Nat Rev Genet. 2005 Sep;6(9):688-98
references: 16151374
references: Plant J. 2007 Sep;51(5):840-9
references: 17573799
references: Cell. 2009 Aug 21;138(4):750-9
references: 19703400
references: Dev Cell. 2005 Apr;8(4):517-27
references: 15809034
references: Plant Cell. 2010 Jul;22(7):2156-70
references: 20675573