title: [Clinical application studies on AFP-L3 detected by micro-spin column method].
abstract: OBJECTIVE
  To explore the clinical value of Lens culinaris agglutinin-reactive alpha-fetoprotein in the differentiation diagnosis between benign and malignant liver diseases, as well as the early warning of hepatocellular carcinoma.
  
  
  METHODS
  Alpha-fetoprotein variants from 300 patients with liver diseases were isolated with micro-spin column equipped lens culinaris agglutinin (LCA). The AFP and AFP-L3 were detected by the electrochemical luminescence (ECL) method, and the proportions of AFP-L3 were calculated.
  
  
  RESULTS
  The positive rates of AFP-L3 of HCC patients and chronic liver disease patients were 95% and 64% respectively, there were significant difference in two groups (chi2 = 134.72, P < 0.01), the HCC incidence rates of AFP-L3 positive and negative chronic liver disease patients showed significant difference (chi2 = 80.158, P < 0.01). there were no correlations between the proportion of AFP-L3 and AFP consistency(r = 0.046, P > 0.05).
  
  
  CONCLUSIONS
  The detection of AFP-L3 by micro-spin column assay show great clinical value in the differentiation diagnosis of benign and malignant liver diseases, as well as the early warning of hepatocellular carcinoma.
authors: The Sixth People Hospital of Hangzhou, Hangzhou 310014, China.
authors: Jin
authors: J
authors: Gao
authors: Ai-Fang
authors: AF
authors: Xu
authors: Hong-Ying
authors: HY
authors: Zheng
authors: Gang
authors: G
authors: Chen
authors: Miao-Chan
authors: MC
authors: Wang
authors: Guo-Qiang
authors: GQ
authors: Lou