title: Interleukin-17F increases the secretion of interleukin-8 and the expression of cyclooxygenase 2 in endometriosis.
abstract: OBJECTIVE
  To examine the effects of interleukin (IL)-17F on the secretion of IL-8 and the gene expression of cyclooxygenase 2 (COX2) in endometriotic stromal cells.
  
  
  METHODS
  In vitro experimental study using human samples.
  
  
  METHODS
  University hospital.
  
  
  METHODS
  Endometriotic tissues were obtained from women with ovarian endometriomas undergoing laparoscopic surgery.
  
  
  METHODS
  Endometriotic stromal cells (ESCs) were cultured with IL-17F.
  
  
  METHODS
  Concentrations of IL-8 were measured by a specific ELISA, and messenger RNA levels of IL-8 and COX2 were measured by real-time reverse transcription-polymerase chain reaction (PCR).
  
  
  RESULTS
  IL-17F increased the secretion of IL-8 from ESCs, and the effect was inhibited by antibodies for IL-17 receptor A and IL-17 receptor C. Tumor necrosis factor α (TNF-α) synergistically enhanced IL-17F-induced increase in IL-8 secretion from ESCs. The IL-17F increased the gene expression of IL-8 and COX2 in ESCs.
  
  
  CONCLUSIONS
  These findings suggest that IL-17F may stimulate the development of endometriosis by up-regulation of IL-8 and COX2.
authors: Department of Obstetrics and Gynecology, University of Tokyo, Tokyo, Japan.
authors: Tetsuya
authors: T
authors: Hirata
authors: Yutaka
authors: Y
authors: Osuga
authors: Masashi
authors: M
authors: Takamura
authors: Ako
authors: A
authors: Saito
authors: Akiko
authors: A
authors: Hasegawa
authors: Kaori
authors: K
authors: Koga
authors: Osamu
authors: O
authors: Yoshino
authors: Yasushi
authors: Y
authors: Hirota
authors: Miyuki
authors: M
authors: Harada
authors: Yuji
authors: Y
authors: Taketani