title: Orientation of GST-tagged lectins via in situ surface modification to create an expanded lectin microarray for glycomic analysis.
abstract: Herein we describe the orientation of GST-tagged lectins on NHS-activated slides via a one-step deposition of the protein and a glutathione (GSH) scaffold. This technology overcomes the need for a premade GSH-surface to orient GST-tagged proteins, enabling us to rapidly expand the analytical capacity of lectin microarrays through addition of oriented lectins, while maintaining lectin diversity.
authors: Department of Chemistry and Biochemistry, University of Texas at Austin, 1 University Station A5300, Austin, TX 78712-0265, USA.
authors: Daniel C
authors: DC
authors: Propheter
authors: Lara K
authors: LK
authors: Mahal