title: Generation of a monoclonal antibody reactive to prefusion myocytes.
abstract: We established a novel monoclonal antibody, Yaksa that is specific to a subpopulation of myogenic cells. The Yaksa antigen is not expressed on the surface of growing myoblasts but only on a subpopulation of myogenin-positive myocytes. When Yaksa antigen-positive mononucleated cells were freshly prepared from a murine myogenic cell by a cell sorter, they fused with each other and formed multinucleated myotubes shortly after replating while Yaksa antigen-negative cells scarcely generated myotubes. These results suggest that Yaksa could segregate fusion-competent, mononucleated cells from fusion-incompetent cells during muscle differentiation. The Yaksa antigen was also expressed in developing muscle and regenerating muscle in vivo and it was localized at sites of cell-cell contact between mono-nucleated muscle cells and between mono-nucleated muscle cells and myotubes. Thus, Yaksa that marks prefusion myocytes before myotube formation can be a useful tool to elucidate the cellular and molecular mechanisms of myogenic cell fusion.
authors: Field of Growth Regulation, Institute for Frontier Medical Sciences, Kyoto University, Sakyo-ku, Kyoto, Japan. kurisaki@frontier.kyoto-u.ac.jp
authors: Tomohiro
authors: T
authors: Kurisaki
authors: Aki
authors: A
authors: Masuda
authors: Shiho
authors: S
authors: Nakagiri
authors: Yoshihiro
authors: Y
authors: Hayata
authors: Motoki
authors: M
authors: Kuhara
authors: Yoshiro
authors: Y
authors: Kishi
authors: Atsuko
authors: A
authors: Sehara-Fujisawa
references: J Supramol Struct. 1977;7(3-4):323-38
references: 357843
references: Biochem Biophys Res Commun. 1993 Mar 15;191(2):351-6
references: 8384837
references: Cold Spring Harb Symp Quant Biol. 2008;73:317-22
references: 19204065
references: J Immunol. 2000 Dec 1;165(11):6037-46
references: 11086035
references: Curr Opin Genet Dev. 2001 Aug;11(4):440-8
references: 11448631
references: Biochem Biophys Res Commun. 2004 Sep 3;321(4):1050-61
references: 15358135
references: J Cell Biol. 1996 Feb;132(4):657-66
references: 8647896
references: Cell Tissue Res. 2005 Feb;319(2):289-97
references: 15549398
references: Dev Biol. 2010 May 1;341(1):66-83
references: 19932206
references: Nature. 1993 Aug 5;364(6437):532-5
references: 8393146
references: Dev Dyn. 2007 Feb;236(2):404-15
references: 17146786
references: Cold Spring Harb Symp Quant Biol. 2008;73:323-31
references: 19329572
references: J Cell Sci. 1998 Mar;111 ( Pt 6):769-79
references: 9472005
references: Dev Dyn. 2009 Jun;238(6):1513-25
references: 19418445
references: Development. 2004 Nov;131(21):5481-90
references: 15469979
references: Curr Opin Genet Dev. 2006 Oct;16(5):525-32
references: 16930987
references: J Cell Biol. 1996 Jan;132(1-2):181-93
references: 8567722
references: Development. 2002 Jun;129(12):2987-95
references: 12050145
references: Am J Pathol. 2003 Jul;163(1):203-15
references: 12819025
references: Science. 1995 Feb 17;267(5200):1024-7
references: 7863329
references: Exp Cell Res. 2010 Nov 1;316(18):3007-13
references: 20580706
references: Exp Cell Res. 2010 Nov 1;316(18):3042-9
references: 20471976
references: Exp Cell Res. 2009 Oct 15;315(17):3052-63
references: 19615358
references: Development. 2007 Sep;134(17):3145-53
references: 17670792
references: Exp Cell Res. 2001 May 1;265(2):212-20
references: 11302686
references: Methods Mol Biol. 2008;475:115-33
references: 18979241
references: Int J Biochem Cell Biol. 2003 Aug;35(8):1151-6
references: 12757751
references: Cells Tissues Organs. 2004;176(1-3):67-78
references: 14745236
references: Nature. 1977 Dec 22-29;270(5639):725-7
references: 563524
references: Mol Biol Cell. 2007 May;18(5):1734-43
references: 17332503
references: Exp Cell Res. 2008 Apr 15;314(7):1553-65
references: 18308302
references: J Cell Biol. 1990 Sep;111(3):1149-59
references: 2167895
references: Science. 1995 Feb 17;267(5200):1018-21
references: 7863327