title: Production of camptothecin in cultures of Chonemorpha grandiflora.
abstract: BACKGROUND
  Chonemorpha grandiflora (Syn. Chonemorpha fragrans (Apocynaceae) is an endangered medicinal plant. It is used in different preparations, such as sudarsanasavam and kumaryasavam used in Kerala Ayurvedic system. C. grandiflora is used for the treatment of fever and stomach disorders. Phytochemical investigations have revealed the presence of steroidal alkaloids, such as chonemorphine and funtumafrine in C. grandiflora. Camptothecin, a well-known anticancer alkaloid has been detected in ethanolic extracts of stem with bark and callus cultures derived from C. grandiflora.
  
  
  METHODS
  Callus cultures of C. grandiflora were raised on Murashige and Skoog's medium supplemented with 2, 4-D. Stem with bark and callus were used for phytochemical analysis mainly the alkaloids. Detection and identification of camptothecin was carried out using thin-layer chromatography (TLC), high-performance thin-layer chromatography, (HPTLC) and high-performance liquid chromatography (HPLC).
  
  
  RESULTS
  An important anticancer alkaloid, camptothecin was detected in ethanolic extracts of stem with bark and callus cultures of C. grandiflora. camptothecin content was 0.013 mg/g in stem with bark and 0.003 mg/g in callus.
  
  
  CONCLUSIONS
  This is the first report on in vivo and in vitro production of camptothecin in C. grandiflora. Camptothecin is known to occur only in six plant sources so, alternative sources for camptothecin are needed. Thus of C. grandiflora could be a new promising alternative source of camptothecin.
authors: Department of Botany, University of Pune, Pune -411007, Maharashtra, India.
authors: A V
authors: AV
authors: Kulkarni
authors: A A
authors: AA
authors: Patwardhan
authors: U
authors: U
authors: Lele
authors: N P
authors: NP
authors: Malpathak
references: J Nat Prod. 1979 Sep-Oct;42(5):475-7
references: 521817
references: J Nat Prod. 1997 Jun;60(6):618-9
references: 9214735
references: Biochem Pharmacol. 1998 Mar 1;55(5):709-11
references: 9515582
references: AIDS Res Hum Retroviruses. 1991 Jan;7(1):65-72
references: 1707642
references: Proc Natl Acad Sci U S A. 1995 Apr 25;92(9):3726-30
references: 7731973
references: Planta Med. 2001 Mar;67(2):150-2
references: 11301862
references: Gan To Kagaku Ryoho. 1991 Aug;18(10):1681-9
references: 1872624
references: Parasitol Res. 1987;74(1):30-3
references: 2894025
references: Cancer Res. 1994 Mar 15;54(6):1431-9
references: 8137244