title: Minichromosome Maintenance Protein 7 is a potential therapeutic target in human cancer and a novel prognostic marker of non-small cell lung cancer.
abstract: BACKGROUND
  The research emphasis in anti-cancer drug discovery has always been to search for a drug with the greatest antitumor potential but fewest side effects. This can only be achieved if the drug used is against a specific target located in the tumor cells. In this study, we evaluated Minichromosome Maintenance Protein 7 (MCM7) as a novel therapeutic target in cancer.
  
  
  RESULTS
  Immunohistochemical analysis showed that MCM7 was positively stained in 196 of 331 non-small cell lung cancer (NSCLC), 21 of 29 bladder tumor and 25 of 70 liver tumor cases whereas no significant staining was observed in various normal tissues. We also found an elevated expression of MCM7 to be associated with poor prognosis for patients with NSCLC (P = 0.0055). qRT-PCR revealed a higher expression of MCM7 in clinical bladder cancer tissues than in corresponding non-neoplastic tissues (P < 0.0001), and we confirmed that a wide range of cancers also overexpressed MCM7 by cDNA microarray analysis. Suppression of MCM7 using specific siRNAs inhibited incorporation of BrdU in lung and bladder cancer cells overexpressing MCM7, and suppressed the growth of those cells more efficiently than that of normal cell strains expressing lower levels of MCM7.
  
  
  CONCLUSIONS
  Since MCM7 expression was generally low in a number of normal tissues we examined, MCM7 has the characteristics of an ideal candidate for molecular targeted cancer therapy in various tumors and also as a good prognostic biomarker for NSCLC patients.
authors: Laboratory of Molecular Medicine, Human Genome Center, Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.
authors: Gouji
authors: G
authors: Toyokawa
authors: Ken
authors: K
authors: Masuda
authors: Yataro
authors: Y
authors: Daigo
authors: Hyun-Soo
authors: HS
authors: Cho
authors: Masanori
authors: M
authors: Yoshimatsu
authors: Masashi
authors: M
authors: Takawa
authors: Shinya
authors: S
authors: Hayami
authors: Kazuhiro
authors: K
authors: Maejima
authors: Makoto
authors: M
authors: Chino
authors: Helen I
authors: HI
authors: Field
authors: David E
authors: DE
authors: Neal
authors: Eiju
authors: E
authors: Tsuchiya
authors: Bruce A J
authors: BA
authors: Ponder
authors: Yoshihiko
authors: Y
authors: Maehara
authors: Yusuke
authors: Y
authors: Nakamura
authors: Ryuji
authors: R
authors: Hamamoto
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