title: Overexpressed cyclin D1 protein related to p53 and retinoblastoma proteins in transitional cell carcinoma of the urinary tract.
abstract: We employed immunohistochemical methods to investigate the overexpression of cyclin D1 protein as well as p53 protein and retinoblastoma protein (pRb) in 57 patients with transitional cell carcinoma (TCC) of the urinary tract, in order to determine their frequency and significance, and also association with the clinicopathological behavior of this tumor. Nuclear immunostaining with cyclin D1 antibody revealed 7 homogeneously stained tumors (12.3%), including 4 heterogeneous pRb expressions, of which only one was also positive for p53. Positive immunostaining with each antibody to p53 or pRb was obtained in 15 (26.3%) and 49 (86%) tumors, respectively. The 49 pRb-positive tumors included 6 homogeneous and 43 heterogeneous nuclear immunostaining patterns. The remaining pRb-negative 8 tumors included 5 cyclin D1-negative tumors. The prevalence of p53 staining was higher in high-grade (P<0.05) and invasive types of tumors (P<0.05) than the other types studied. On the other hand, no significant relationship was found between cyclin D1- or pRb-immunoreactivity and clinicopathological factors. These findings provide evidence for infrequent cyclin D1 protein overexpression in TCC independently associated with p53 protein and/or pRb overexpression.
authors: UNIV TOKUSHIMA,SCH MED,DEPT UROL,TOKUSHIMA 770,JAPAN. KOCHI MED SCH,DEPT PATHOL 2,NANKO KU,KOCHI 783,JAPAN.
authors: K
authors: K
authors: Ohta
authors: M
authors: M
authors: Kuwahara
authors: N
authors: N
authors: Fujisaki
authors: S
authors: S
authors: Kagawa
authors: M
authors: M
authors: Furihata
authors: Y
authors: Y
authors: Ohtsuki