title: Ethanol extract of Elaeocarpus petiolatus inhibits lipopolysaccharide-induced inflammation in macrophage cells.
abstract: Elaeocarpus petiolatus is known to exert active oxygen scavenging, anti-aging, and whitening actions. However, the biological effects of E. petiolatus on inflammation and the underlying mechanisms are yet to be established. In the present study, we investigated the anti-inflammatory effects of the ethanol extract from E. petiolatus (EPE) bark in murine Raw264.7 macrophages stimulated with lipopolysaccharide (LPS). EPE inhibited the production of PGE(2), TNF-α, and IL-1β in a dose-dependent manner in Raw264.7 cells stimulated with LPS. The decrease in PGE(2) production was correlated with reduced COX-2 expression. Furthermore, EPE suppressed the phosphorylation of extracellular signal-related kinases (ERK), c-Jun N-terminal kinase (JNK), and p38 as well as translocation of the NF-κB p65 subunit from the cytosol to nucleus. Our results suggest that EPE exerts anti-inflammatory activity through inhibition of inflammatory mediators, such as PGE(2), TNF-α, and IL-1β, and downregulation of COX-2 via suppression of NF-κB translocation and phosphorylation of ERK, JNK, and p38 in LPS-stimulated Raw264.7 cells.
authors: Immune Modulator Research Center, Korea Research Institute of Bioscience and Biotechnology, 685-4 Yangchung-ri, Ochang-eup, Cheongwon-gun, Chungbuk, Republic of Korea.
authors: Ok-Kyoung
authors: OK
authors: Kwon
authors: Kyung-Seop
authors: KS
authors: Ahn
authors: Ji-Won
authors: JW
authors: Park
authors: Ha-Young
authors: HY
authors: Jang
authors: Hyouk
authors: H
authors: Joung
authors: Hyeong-Kyu
authors: HK
authors: Lee
authors: Sei-Ryang
authors: SR
authors: Oh
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