title: Development of microsatellite markers in Lupinus luteus (Fabaceae) and cross-species amplification in other lupine species.
abstract: OBJECTIVE
  Microsatellite primers were developed in Lupinus luteus L., an emerging temperate protein crop, to investigate genetic diversity, population structure, and to facilitate the generation of better yellow lupine varieties. •
  
  
  RESULTS
  Thirteen polymorphic primer sets were evaluated in a European and Eastern European accession collection of L. luteus. The primers amplified di-, tri-, and tetranucleotide repeats with 2-4 alleles per locus. These revealed a moderate to low level of genetic variation, as indicated by an average observed heterozygosity of 0.0126. Select loci also amplified successfully in the closely related species L. hispanicus Boiss. & Reut. and in the New World species L. mutabilis Sweet. •
  
  
  CONCLUSIONS
  These results indicate the utility of primers for the study of genetic diversity across L. luteus populations and related lupine species. The use of these microsatellite markers will facilitate the implementation of several molecular breeding strategies in yellow lupine.
authors: Agro Aquaculture Nutritional Genomic Center (CGNA), Plant Biotechnology Unit INIA-Carillanca P.O. Box 58-D, Temuco, Chile.
authors: Lorena B Parra
authors: LB
authors: Gonzalez
authors: Shannon C K
authors: SC
authors: Straub
authors: Jeff J
authors: JJ
authors: Doyle
authors: Paula E Mora
authors: PE
authors: Ortega
authors: Haroldo E Salvo
authors: HE
authors: Garrido
authors: Iván J Maureira
authors: IJ
authors: Butler