title: Increased expression of interleukin-22 by synovial Th17 cells during late stages of murine experimental arthritis is controlled by interleukin-1 and enhances bone degradation.
abstract: OBJECTIVE
  Interleukin-22 (IL-22) is a mediator in antimicrobial responses and inflammatory autoimmune diseases. Although IL-22 and its receptor, IL-22R, have been identified in the synovium of rheumatoid arthritis patients, the source of IL-22 and its contribution to disease pathogenicity remain to be established. This study was undertaken to investigate the regulation of IL-22 by Th17 cells in vitro and to evaluate the potential for IL-22 depletion in an experimental arthritis model using mice deficient in the IL-1 receptor antagonist (IL-1Ra-/-).
  
  
  METHODS
  Naive murine T cells were cultured under conditions leading to polarization of the cells into subsets of Th1, Th2, induced Treg, and Th17. Cytokines were measured in the culture supernatants, and the cells were analyzed by fluorescence-activated cell sorting. Tissue samples from the inflamed ankle synovium of IL-1Ra-/- mice were isolated, and messenger RNA levels of marker genes were quantified. IL-1Ra-/- mice were treated with neutralizing anti-IL-22 antibodies. Synovial cells were isolated from the inflamed tissue and sorted into fractions for analysis of cytokine production.
  
  
  RESULTS
  In vitro tests showed that Th17 cells produced high levels of IL-22 after stimulation with IL-1 or IL-23. Interestingly, a synergistic increase in the production of IL-22 was observed after combining IL-1 and IL-23. In vivo, IL-1Ra-/- mice displayed a progressive erosive arthritis, characterized by up-regulation of IL-17 in mildly and severely inflamed tissue, whereas the levels of IL-22 and IL-22R were increased only in severely inflamed synovia. Anti-IL-22 treatment of IL-1Ra-/- mice significantly reduced the inflammation and bone erosion. Analysis of isolated single cells from the inflamed synovia revealed that IL-22 was mainly produced by IL-17-expressing T cells.
  
  
  CONCLUSIONS
  These findings suggest that IL-22 plays an important role in IL-1-driven chronic joint destruction.
authors: Radboud University Nijmegen Medical Centre, Department of Rheumatology, Rheumatology Research and Advanced Therapeutics, Nijmegen, The Netherlands. RJMarijnissen@reuma.umcn.nl
authors: Renoud J
authors: RJ
authors: Marijnissen
authors: Marije I
authors: MI
authors: Koenders
authors: Ruben L
authors: RL
authors: Smeets
authors: Mark H T
authors: MH
authors: Stappers
authors: Cheryl
authors: C
authors: Nickerson-Nutter
authors: Leo A B
authors: LA
authors: Joosten
authors: Annemieke M H
authors: AM
authors: Boots
authors: Wim B
authors: WB
authors: van den Berg