title: Purification of proteins containing zinc finger domains using immobilized metal ion affinity chromatography.
abstract: Heterologous proteins are frequently purified by immobilized metal ion affinity chromatography (IMAC) based on their modification with a hexa-histidine affinity tag (His-tag). The terminal His-tag can, however, alter functional properties of the tagged protein. Numerous strategies for the tag removal have been developed including chemical treatment and insertion of protease target sequences in the protein sequence. Instead of using these approaches, we took an advantage of natural interaction of zinc finger domains with metal ions to purify functionally similar retroviral proteins from two different retroviruses. We found that these proteins exhibited significantly different affinities to the immobilized metal ions, despite that both contain the same type of zinc finger motif (i.e., CCHC). While zinc finger proteins may differ in biochemical properties, the multitude of IMAC platforms should allow relatively simple yet specific method for their isolation in native state.
authors: Department of Biochemistry and Microbiology, Institute of Chemical Technology, Prague, Czech Republic. irena.vorackova@vscht.cz
authors: Irena
authors: I
authors: Voráčková
authors: Sárka
authors: S
authors: Suchanová
authors: Pavel
authors: P
authors: Ulbrich
authors: William E
authors: WE
authors: Diehl
authors: Tomáš
authors: T
authors: Ruml
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