title: Orientia tsutsugamushi stimulates an original gene expression program in monocytes: relationship with gene expression in patients with scrub typhus.
abstract: Orientia tsutsugamushi is the causal agent of scrub typhus, a public health problem in the Asia-Pacific region and a life-threatening disease. O. tsutsugamushi is an obligate intracellular bacterium that mainly infects endothelial cells. We demonstrated here that O. tsutsugamushi also replicated in monocytes isolated from healthy donors. In addition, O. tsutsugamushi altered the expression of more than 4,500 genes, as demonstrated by microarray analysis. The expression of type I interferon, interferon-stimulated genes and genes associated with the M1 polarization of macrophages was significantly upregulated. O. tsutsugamushi also induced the expression of apoptosis-related genes and promoted cell death in a small percentage of monocytes. Live organisms were indispensable to the type I interferon response and apoptosis and enhanced the expression of M1-associated cytokines. These data were related to the transcriptional changes detected in mononuclear cells isolated from patients with scrub typhus. Here, the microarray analyses revealed the upregulation of 613 genes, which included interferon-related genes, and some features of M1 polarization were observed in these patients, similar to what was observed in O. tsutsugamushi-stimulated monocytes in vitro. This is the first report demonstrating that monocytes are clearly polarized in vitro and ex vivo following exposure to O. tsutsugamushi. These results would improve our understanding of the pathogenesis of scrub typhus, during which interferon-mediated activation of monocytes and their subsequent polarization into an M1 phenotype appear critical. This study may give us a clue of new tools for the diagnosis of patients with scrub typhus.
authors: Unité de Recherche sur les Maladies Infectieuses Tropicales et Emergentes, Centre National de la Recherche Scientifique - Institut de Recherche pour le Développement Unité Mixte de Recherche 6236, Université de la Méditerranée, Faculté de Médecine, Marseille, France.
authors: Wiwit
authors: W
authors: Tantibhedhyangkul
authors: Thanavadee
authors: T
authors: Prachason
authors: Duangdao
authors: D
authors: Waywa
authors: Adil
authors: A
authors: El Filali
authors: Eric
authors: E
authors: Ghigo
authors: Wanna
authors: W
authors: Thongnoppakhun
authors: Didier
authors: D
authors: Raoult
authors: Yupin
authors: Y
authors: Suputtamongkol
authors: Christian
authors: C
authors: Capo
authors: Chanin
authors: C
authors: Limwongse
authors: Jean-Louis
authors: JL
authors: Mege
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