title: Optimized genotyping with microsatellite markers in the fungal banana pathogen Mycosphaerella fijiensis (Mycosphaerellaceae).
abstract: OBJECTIVE
  Large-scale population genetics studies are required to investigate the dispersal processes underlying the emergence of Mycosphaerella fijiensis, a fungal pathogen of banana. To this end, we have developed an optimized genotyping procedure combining novel microsatellite markers and a modified DNA extraction protocol. •
  
  
  RESULTS
  Primers for tetranucleotide loci were designed directly from the recently published genome sequence of M. fijiensis. A total of 19 new polymorphic and easy-to-score markers were developed. Their use was combined with an adapted protocol for total DNA extraction starting from young lesions collected from banana leaves, thus avoiding a pathogen isolation step. •
  
  
  CONCLUSIONS
  The combination of the two technical developments presented here will permit the expansion of genotyping capacity in M. fijiensis, allowing large-scale analysis of samples from various geographic locations.
authors: CIRAD, Unité Mixte de Recherche-Biologie et Génétique des Interactions Plante-Parasite, TA A-54/K, Campus International de Baillarguet, F-34398 Montpellier Cedex 5, France.
authors: Stéphanie
authors: S
authors: Robert
authors: Adrien
authors: A
authors: Rieux
authors: Xavier
authors: X
authors: Argout
authors: Jean
authors: J
authors: Carlier
authors: Marie-Françoise
authors: MF
authors: Zapater