title: In vitro imaging and human serum albumin responsive dimeric lanthanide DO3A complex.
abstract: Two series of dimeric DO3A (1,4,7,10-tetraazacyclodecane-1,4,7-triacetate) lanthanide complexes (LnL(1)-LnL(2), Ln = Eu, Gd, and Tb) have been synthesized with two different bridged chromophores. The X-ray structures of dimeric LnL(1) (Ln = Gd and Tb) complexes show that each metal ion has nine coordination numbers with eight directly bound donor atoms of the ligand and one oxygen donor from the water molecule. Photophysical measurements indicate that the bridged antenna in LnL(2) gives a higher efficiency than that of LnL(1) and is responsive to the protein Human Serum Albumin (HSA), giving an f-f luminescence signal enhancement with a binding constant log K = 4.84. In vitro imaging of EuL(1) and EuL(2) in HeLa cells has been recorded, and EuL(2) has demonstrated a higher rate of cellular uptake and low cytotoxicity (IC(50) = 3 mM).
authors: Department of Applied Biology and Chemical Technology, Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong SAR.
authors: Yuen On
authors: YO
authors: Fung
authors: Wanqing
authors: W
authors: Wu
authors: Chi-Tung
authors: CT
authors: Yeung
authors: Hoi-Kuan
authors: HK
authors: Kong
authors: Kenny Kam-Cheng
authors: KK
authors: Wong
authors: Wai-Sum
authors: WS
authors: Lo
authors: Ga-Lai
authors: GL
authors: Law
authors: Ka-Leung
authors: KL
authors: Wong
authors: Chi-Kong
authors: CK
authors: Lau
authors: Chi-Sing
authors: CS
authors: Lee
authors: Wing-Tak
authors: WT
authors: Wong