title: Fast analysis of recombinant monoclonal antibodies using IdeS proteolytic digestion and electrospray mass spectrometry.
abstract: We describe a fast and informative method to investigate the posttranslational modifications of monoclonal antibodies (MAbs). The MAb is first digested by a specific enzyme that cleaves heavy chains under the hinge domain. After reduction of disulfide bridges, three polypeptide chains of approximately 25 kDa are released and analyzed by liquid chromatography-mass spectrometry (LC-MS). By bisecting the heavy chains prior to MS analysis, this method provides a better MS resolution and facilitates the study of the N-linked glycans as well as of other modifications (loss of C-terminal lysine, pyroglutamination, and oxidation). The sample preparation and analysis can be performed within few hours.
authors: Analytical Department, LFB Biotechnology, 3 Avenue des Tropiques, 91958 Courtaboeuf (Les Ulis), France. chevreuxg@lfb.fr
authors: Guillaume
authors: G
authors: Chevreux
authors: Nolwenn
authors: N
authors: Tilly
authors: Nicolas
authors: N
authors: Bihoreau