title: Factors determining the superior performance of lipid/DNA/protammine nanoparticles over lipoplexes.
abstract: The utility of using a protammine/DNA complex coated with a lipid envelope made of cationic 1,2-dioleoyl-3-trimethylammonium propane (DOTAP) for transfecting CHO (Chinese hamster ovary cells), HEK293 (human embryonic kidney cells), NIH 3T3 (mouse embryonal cells), and A17 (murine cancer cells) cells was examined. The widely used DOTAP/DNA lipoplex was employed as a reference. In all the tested cell lines lipid/protamine/DNA (LPD) nanoparticles were more efficient in transfecting cells than lipoplexes even though the lipid composition of the lipid envelope was the same in both devices. Physical-chemical properties were found to control the ability of nanocarriers to release DNA upon interaction with cellular membranes. LPD complexes easily release their DNA payload, while lipoplexes remain largely intact and accumulate at the cell nucleus. Collectively, these data explain why LPD nanoparticles often exhibit superior performances compared to lipoplexes in trasfecting cells and represent a promising class of nanocarriers for gene delivery.
authors: Department of Molecular Medicine, Sapienza University of Rome, Viale Regina Elena, 324, 00161, Rome, Italy. giulio.caracciolo@uniroma1.it
authors: Giulio
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authors: Caracciolo
authors: Daniela
authors: D
authors: Pozzi
authors: Anna Laura
authors: AL
authors: Capriotti
authors: Carlotta
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authors: Marianecci
authors: Maria
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authors: Carafa
authors: Cristina
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authors: Marchini
authors: Maura
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authors: Montani
authors: Augusto
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authors: Amici
authors: Heinz
authors: H
authors: Amenitsch
authors: Michelle A
authors: MA
authors: Digman
authors: Enrico
authors: E
authors: Gratton
authors: Susana S
authors: SS
authors: Sanchez
authors: Aldo
authors: A
authors: Laganà