title: Spatiotemporal changes in the structure and composition of a less-abundant bacterial phylum (Planctomycetes) in two perialpine lakes.
abstract: We used fingerprinting and cloning-sequencing to study the spatiotemporal dynamics and diversity of Planctomycetes in two perialpine lakes with contrasting environmental conditions. Planctomycetes, which are less-abundant bacteria in freshwater ecosystems, appeared to be structured in the same way as the entire bacterial community in these ecosystems. They were more diversified and displayed fewer temporal variations in the hypolimnia than in the epilimnia. Like the more-abundant bacterial groups in aquatic systems, Planctomycetes communities seem to be composed of a very small number of abundant and widespread operational taxonomic units (OTUs) and a large number of OTUs that are present at low abundance. This indicates that the concept of "abundant or core" and "rare" bacterial phylotypes could also be applied to less-abundant freshwater bacterial phyla. The richness and diversity of Planctomycetes were mainly driven by pH and were similar in both of the lakes studied, whereas the composition of the Planctomycetes community seemed to be determined by a combination of factors including temperature, pH, and nutrients. The relative abundances of the dominant OTUs varied over time and were differently associated with abiotic factors. Our findings demonstrate that less-abundant bacterial phyla, such as Planctomycetes, can display strong spatial and seasonal variations linked to environmental conditions and suggest that their functional role in the lakes studied might be attributable mainly to a small number of phylotypes and vary over space and time in the water column.
authors: INRA-UMR CARRTEL, BP511, 74203 Thonon-les-Bains Cedex, France.
authors: Thomas
authors: T
authors: Pollet
authors: Rémy D
authors: RD
authors: Tadonléké
authors: Jean François
authors: JF
authors: Humbert
references: Environ Microbiol. 2006 Oct;8(10):1857-63
references: 16958766
references: Bioinformatics. 2004 Sep 22;20(14):2317-9
references: 15073015
references: Appl Environ Microbiol. 2000 Nov;66(11):5053-65
references: 11055963
references: FEMS Microbiol Ecol. 2007 Mar;59(3):543-55
references: 17059481
references: Microbiol Rev. 1987 Jun;51(2):221-71
references: 2439888
references: Environ Microbiol. 2001 Jul;3(7):450-9
references: 11553235
references: Environ Microbiol. 2002 Nov;4(11):634-43
references: 12460271
references: J Bacteriol. 1992 Aug;174(15):5072-8
references: 1629164
references: Appl Environ Microbiol. 2005 Jan;71(1):227-39
references: 15640192
references: Nature. 2003 Apr 10;422(6932):608-11
references: 12686999
references: Appl Environ Microbiol. 1997 Apr;63(4):1375-81
references: 9097434
references: Environ Microbiol. 2009 Sep;11(9):2339-50
references: 19508336
references: Environ Microbiol. 2010 May;12(5):1132-43
references: 20132284
references: Appl Environ Microbiol. 2004 Sep;70(9):5094-101
references: 15345387
references: J Bacteriol. 1987 Jun;169(6):2702-7
references: 3584067
references: Environ Microbiol. 2008 Apr;10(4):1057-67
references: 18218031
references: Appl Environ Microbiol. 1995 Jul;61(7):2798-801
references: 7618894
references: Environ Microbiol. 2008 Nov;10(11):3106-19
references: 18510553
references: Proc Natl Acad Sci U S A. 2006 Jan 17;103(3):626-31
references: 16407148
references: Microb Ecol. 2001 Dec;42(4):598-605
references: 12024242
references: Proc Natl Acad Sci U S A. 2009 Dec 29;106(52):22427-32
references: 20018741
references: Environ Microbiol. 2009 Sep;11(9):2412-24
references: 19558513
references: Environ Microbiol. 2004 Mar;6(3):242-53
references: 14871208
references: ISME J. 2008 Apr;2(4):379-92
references: 18340335
references: Appl Environ Microbiol. 1997 Jul;63(7):2647-53
references: 9212415
references: Appl Environ Microbiol. 2008 Mar;74(6):1856-68
references: 18245245
references: Environ Microbiol Rep. 2011 Apr;3(2):254-61
references: 23761258
references: Trends Microbiol. 2006 Jun;14(6):257-63
references: 16679014
references: Proc Natl Acad Sci U S A. 2007 Dec 18;104(51):20404-9
references: 18077371
references: Appl Environ Microbiol. 2002 Oct;68(10):4740-50
references: 12324315
references: Nucleic Acids Res. 2005 Jan 1;33(Database issue):D294-6
references: 15608200
references: Appl Environ Microbiol. 1994 Feb;60(2):746-8
references: 8135524
references: Nature. 1999 Jul 29;400(6743):446-9
references: 10440372
references: Appl Environ Microbiol. 2003 Dec;69(12):7354-63
references: 14660385
references: Environ Microbiol. 2001 Nov;3(11):680-90
references: 11846758
references: Proc Natl Acad Sci U S A. 2005 Oct 11;102(41):14683-8
references: 16186488
references: FEMS Microbiol Ecol. 2006 Oct;58(1):109-19
references: 16958912