title: Cell uptake of a biosensor detected by hyperpolarized 129Xe NMR: the transferrin case.
abstract: For detection of biological events in vitro, sensors using hyperpolarized (129)Xe NMR can become a powerful tool, provided the approach can bridge the gap in sensitivity. Here we propose constructs based on the non-selective grafting of cryptophane precursors on holo-transferrin. This biological system was chosen because there are many receptors on the cell surface, and endocytosis further increases this density. The study of these biosensors with K562 cell suspensions via fluorescence microscopy and (129)Xe NMR indicates a strong interaction, as well as interesting features such as the capacity of xenon to enter the cryptophane even when the biosensor is endocytosed, while keeping a high level of polarization. Despite a lack of specificity for transferrin receptors, undoubtedly due to the hydrophobic character of the cryptophane moiety that attracts the biosensor into the cell membrane, these biosensors allow the first in-cell probing of biological events using hyperpolarized xenon.
authors: CEA, IRAMIS, SIS2M, Laboratoire Structure et Dynamique par Résonance Magnétique, UMR CEA/CNRS 3299, Gif sur Yvette, France.
authors: Céline
authors: C
authors: Boutin
authors: Antoine
authors: A
authors: Stopin
authors: Fatimazohra
authors: F
authors: Lenda
authors: Thierry
authors: T
authors: Brotin
authors: Jean-Pierre
authors: JP
authors: Dutasta
authors: Nadège
authors: N
authors: Jamin
authors: Alain
authors: A
authors: Sanson
authors: Yves
authors: Y
authors: Boulard
authors: François
authors: F
authors: Leteurtre
authors: Gaspard
authors: G
authors: Huber
authors: Aurore
authors: A
authors: Bogaert-Buchmann
authors: Nawal
authors: N
authors: Tassali
authors: Hervé
authors: H
authors: Desvaux
authors: Marie
authors: M
authors: Carrière
authors: Patrick
authors: P
authors: Berthault