title: Comparative protein expression in different strains of the bloom-forming cyanobacterium Microcystis aeruginosa.
abstract: Toxin production in algal blooms presents a significant problem for the water industry. Of particular concern is microcystin, a potent hepatotoxin produced by the unicellular freshwater species Microcystis aeruginosa. In this study, the proteomes of six toxic and nontoxic strains of M. aeruginosa were analyzed to gain further knowledge in elucidating the role of microcystin production in this microorganism. This represents the first comparative proteomic study in a cyanobacterial species. A large diversity in the protein expression profiles of each strain was observed, with a significant proportion of the identified proteins appearing to be strain-specific. In total, 475 proteins were identified reproducibly and of these, 82 comprised the core proteome of M. aeruginosa. The expression of several hypothetical and unknown proteins, including four possible operons was confirmed. Surprisingly, no proteins were found to be produced only by toxic or nontoxic strains. Quantitative proteome analysis using the label-free normalized spectrum abundance factor approach revealed nine proteins that were differentially expressed between toxic and nontoxic strains. These proteins participate in carbon-nitrogen metabolism and redox balance maintenance and point to an involvement of the global nitrogen regulator NtcA in toxicity. In addition, the switching of a previously inactive toxin-producing strain to microcystin synthesis is reported.
authors: School of Biotechnology and Biomolecular Sciences, University of New South Wales, NSW 2052, Australia.
authors: Ralitza
authors: R
authors: Alexova
authors: Paul A
authors: PA
authors: Haynes
authors: Belinda C
authors: BC
authors: Ferrari
authors: Brett A
authors: BA
authors: Neilan
references: Environ Microbiol. 2008 Dec;10(12):3337-48
references: 18759740
references: Brief Funct Genomic Proteomic. 2006 Jun;5(2):121-32
references: 16772275
references: J Bacteriol. 2003 Apr;185(8):2582-91
references: 12670983
references: FEBS Lett. 2000 Dec 29;487(2):272-6
references: 11150523
references: Appl Environ Microbiol. 2000 Jan;66(1):176-9
references: 10618220
references: Environ Microbiol. 2008 Oct;10(10):2476-83
references: 18647335
references: Vet Pathol. 1984 Jan;21(1):102-13
references: 6424323
references: Photosynth Res. 2006 Sep;89(2-3):157-71
references: 16969714
references: BMC Microbiol. 2009 Jul 09;9:135
references: 19589152
references: J Bacteriol. 2008 Nov;190(22):7584-90
references: 18805988
references: BMC Genomics. 2008 Jun 05;9:274
references: 18534010
references: Plant Cell Physiol. 2006 Jun;47(6):679-88
references: 16549396
references: Toxicon. 2008 Mar 1;51(3):388-97
references: 18067936
references: Toxicon. 2009 Jun;53(7-8):754-61
references: 19233225
references: FEBS Lett. 2009 Jun 5;583(11):1753-8
references: 19351534
references: J Proteome Res. 2006 Sep;5(9):2339-47
references: 16944946
references: J Proteome Res. 2007 Feb;6(2):621-35
references: 17269719
references: Mol Microbiol. 2006 Feb;59(3):893-906
references: 16420359
references: Photosynth Res. 2007 Jul-Sep;93(1-3):69-77
references: 17279442
references: Proteomics. 2009 Jun;9(11):3136-55
references: 19526548
references: Appl Environ Microbiol. 1995 Feb;61(2):797-800
references: 7574617
references: Plant Physiol. 2007 Aug;144(4):1946-59
references: 17600135
references: Nat Toxins. 1999;7(6):377-85
references: 11122533
references: Environ Toxicol. 2002;17(4):407-13
references: 12203964
references: Environ Microbiol. 2007 Apr;9(4):965-70
references: 17359268
references: J Proteomics. 2009 Apr 13;72(3):416-38
references: 19185068
references: Toxicol In Vitro. 2008 Oct;22(7):1714-8
references: 18675338
references: Int J Syst Evol Microbiol. 2001 May;51(Pt 3):873-879
references: 11411709
references: Plant Physiol. 2004 Jan;134(1):470-81
references: 14730074
references: Appl Environ Microbiol. 2003 Mar;69(3):1475-81
references: 12620831
references: Appl Environ Microbiol. 2007 May;73(9):2939-46
references: 17337540
references: FEMS Microbiol Rev. 2004 Jun;28(3):319-33
references: 15449606
references: Electrophoresis. 2007 May;28(10):1624-32
references: 17447238
references: J Bacteriol. 2003 May;185(9):2774-85
references: 12700256
references: Rapid Commun Mass Spectrom. 2003;17(20):2310-6
references: 14558131
references: Appl Environ Microbiol. 2005 Oct;71(10):6126-33
references: 16204530
references: Appl Environ Microbiol. 2010 Jul;76(13):4362-8
references: 20453121
references: Microb Ecol. 2002 May;43(4):443-54
references: 11953809
references: Proteomics. 2005 Dec;5(18):4905-16
references: 16287171
references: J Proteome Res. 2008 Nov;7(11):4974-81
references: 18837535
references: Science. 2008 Feb 22;319(5866):1083-6
references: 18292340
references: Lett Appl Microbiol. 2009 Sep;49(3):332-7
references: 19627483
references: J Proteome Res. 2009 Jan;8(1):187-98
references: 19012430
references: J Biol Chem. 2004 Jul 2;279(27):28165-73
references: 15102833
references: Environ Microbiol. 2001 Nov;3(11):669-79
references: 11846757
references: Microbiology (Reading). 2001 Nov;147(Pt 11):3113-9
references: 11700361
references: Appl Environ Microbiol. 2005 Sep;71(9):5177-81
references: 16151102
references: FEMS Microbiol Lett. 2005 Oct 1;251(1):149-54
references: 16112821
references: Analyst. 1994 Jul;119(7):1525-30
references: 7943743
references: Science. 2005 Aug 5;309(5736):936-8
references: 16081736
references: Environ Microbiol. 2005 Jun;7(6):798-805
references: 15892699
references: Toxicon. 2006 Feb;47(2):218-28
references: 16376961
references: Antioxid Redox Signal. 2008 Jul;10(7):1235-74
references: 18377232
references: J Proteome Res. 2008 Apr;7(4):1615-28
references: 18290607
references: FEMS Microbiol Ecol. 2001 Mar;35(1):1-9
references: 11248384
references: Biochem Biophys Res Commun. 2008 Oct 17;375(2):241-6
references: 18706891
references: DNA Res. 2007 Dec 31;14(6):247-56
references: 18192279
references: Nucleic Acids Res. 2001 May 1;29(9):e45
references: 11328886
references: J Proteome Res. 2006 Nov;5(11):3096-104
references: 17081061
references: Electrophoresis. 1997 Aug;18(8):1252-8
references: 9298645
references: J Bacteriol. 2004 Apr;186(8):2355-65
references: 15060038
references: Nucleic Acids Res. 2005 Sep 12;33(16):5156-71
references: 16157864
references: Proteomics. 2008 May;8(10):2089-103
references: 18425732
references: Plant Mol Biol. 2000 May;43(1):23-32
references: 10949371
references: J Agric Food Chem. 2007 Oct 17;55(21):8310-8
references: 17874839
references: Microbiology (Reading). 2007 Nov;153(Pt 11):3838-3851
references: 17975093
references: J Exp Bot. 2003 Feb;54(383):609-22
references: 12554704
references: Bioinformatics. 2004 Jun 12;20(9):1466-7
references: 14976030
references: Environ Microbiol. 2004 Aug;6(8):831-41
references: 15250885
references: Appl Environ Microbiol. 2009 Jan;75(2):366-73
references: 19028911
references: J Biol Chem. 1997 Feb 21;272(8):5087-97
references: 9030574
references: Proc Natl Acad Sci U S A. 2004 Jan 13;101(2):568-73
references: 14701903
references: Environ Toxicol. 2006 Apr;21(2):95-103
references: 16528683
references: Appl Environ Microbiol. 2000 Aug;66(8):3387-92
references: 10919796
references: Photosynth Res. 2005;83(2):135-50
references: 16143848
references: J Proteome Res. 2003 Jan-Feb;2(1):43-50
references: 12643542
references: Mol Microbiol. 2006 Jul;61(2):457-69
references: 16796668
references: J Struct Biol. 2005 Aug;151(2):208-14
references: 16054393
references: FEBS Lett. 1999 Dec 17;463(3):216-20
references: 10606724
references: Electrophoresis. 2006 Apr;27(8):1630-40
references: 16609937
references: Proc Natl Acad Sci U S A. 2006 Jun 27;103(26):9820-5
references: 16777960
references: Plant Cell Physiol. 2001 Jan;42(1):46-53
references: 11158443
references: Physiol Plant. 2007 Sep;131(1):22-32
references: 18251921