title: Eagles report: Developing cancer biomarkers from genome-wide DNA methylation analyses.
abstract: Analyses of DNA methylation in human cancers have identified hypermethylation of individual genes and diminished methylation at repeat elements as common alterations, and have thereby provided important mechanistic insights into cancer biology as well as biomarkers for cancer detection, prognosis and prediction of therapy responses. The techniques available in the past were best suited for investigations of individual candidate genes and sequences, whereas recently developed high-throughput techniques promise to generate unbiased and comprehensive surveys of DNA methylation states across entire genomes. In this minireview we give a short overview of established and novel techniques and outline some major questions that can now be addressed to develop further cancer biomarkers and therapies based on DNA methylation.
authors: Wolfgang A Schulz, Wolfgang Goering, Department of Urology, Medical Faculty, Heinrich Heine University, 40225 Duesseldorf, Germany.
authors: Wolfgang A
authors: WA
authors: Schulz
authors: Wolfgang
authors: W
authors: Goering
references: Methods. 2010 Nov;52(3):248-54
references: 20434562
references: Cancer Res. 2009 Jan 15;69(2):599-608
references: 19147574
references: Proc Natl Acad Sci U S A. 1996 Sep 3;93(18):9821-6
references: 8790415
references: Endocr Relat Cancer. 2006 Jun;13(2):357-77
references: 16728568
references: Genome Res. 2006 Mar;16(3):383-93
references: 16449502
references: Methods. 2010 Nov;52(3):203-12
references: 20430099
references: Int J Cancer. 2008 Dec 15;123(12):2798-807
references: 18803290
references: J Clin Oncol. 2008 Sep 1;26(25):4189-99
references: 18757334
references: Nucleic Acids Res. 1994 Aug 11;22(15):2990-7
references: 8065911
references: Mol Cell Biol. 1999 Nov;19(11):7327-35
references: 10523621
references: Genome Res. 2010 Apr;20(4):440-6
references: 20219944
references: Epigenomics. 2010 Aug;2(4):561-73
references: 22121974
references: Genome Res. 2008 May;18(5):780-90
references: 18316654
references: Mol Cancer. 2008 Jul 10;7:62
references: 18616821
references: J Pathol. 2010 Jan;220(2):231-43
references: 19918804
references: Cancer Cell. 2010 Jul 13;18(1):11-22
references: 20579941
references: Cancer Biol Ther. 2006 Aug;5(8):993-1001
references: 16775427
references: Nat Genet. 2009 Feb;41(2):178-186
references: 19151715
references: Methods Mol Biol. 2009;507:117-30
references: 18987811
references: Mol Cell Biol. 2004 Apr;24(8):3387-95
references: 15060159
references: Nucleic Acids Res. 2000 Apr 15;28(8):E32
references: 10734209
references: Nature. 2007 May 24;447(7143):425-32
references: 17522676
references: Endocrinology. 2009 Sep;150(9):3991-4002
references: 19520778
references: Nature. 2010 Jun 10;465(7299):728-35
references: 20535202
references: Nucleic Acids Res. 1990 Feb 11;18(3):687
references: 1689825
references: Curr Top Microbiol Immunol. 2006;310:211-50
references: 16909913
references: Biochim Biophys Acta. 2009 Dec;1796(2):114-28
references: 19344752
references: Cancer Res. 2010 May 1;70(9):3463-72
references: 20406976
references: Cell. 2007 Feb 23;128(4):683-92
references: 17320506
references: Br J Cancer. 2010 Jan 19;102(2):419-27
references: 20051947
references: Nat Rev Cancer. 2003 Apr;3(4):253-66
references: 12671664
references: Am J Hum Genet. 2010 Mar 12;86(3):420-33
references: 20206335
references: Nature. 2009 Nov 19;462(7271):315-22
references: 19829295
references: Nature. 2010 Sep 16;467(7313):338-42
references: 20720541
references: Nature. 2009 Apr 9;458(7239):719-24
references: 19360079
references: Nat Rev Immunol. 2009 Feb;9(2):91-105
references: 19151746
references: Nucleic Acids Res. 2010 Jan;38(2):391-9
references: 19906696
references: Biochim Biophys Acta. 2007 Jan;1775(1):138-62
references: 17045745
references: Nat Genet. 2006 Jul;38(7):787-93
references: 16804544
references: Methods Mol Biol. 2009;507:131-48
references: 18987812
references: Cancer Invest. 2009 Jun;27(5):549-60
references: 19229700