title: [Evaluation of toxin producing abilities of non-O1/non-O139 serogroup Vibrio cholerae isolated from humans].
abstract: OBJECTIVE
  Determination of non-O1/non-O139 Vibrio cholerae toxin (CT) gene expression by using EIA, and biological effect of non-O1/non-O139 V. cholerae supernatant on cell cultures evaluation.
  
  
  METHODS
  39 V. cholerae strains from various serological groups were studied. Hemolytic activity of strains was determined by using Greig test, and cholera toxin production--in GM1-EIA and in continuous cell lines by registering cytotonic, cytotoxic and proteolitic effect.
  
  
  RESULTS
  GM1-EIA method does not detect CT production in 29 museum strains of non-O1/non-O139 V. cholerae in vitro. CT was detected only in 1 non-O1/non-O139 V. cholerae strain supernatant with OD = 0.577 that is substantially lower than in O1 V. cholerae strains (OD = 2.176). In cell cultures non-O1/non-O139 V. cholerae supernatants diluted to 1:100 caused elongation only in single cells.
  
  
  CONCLUSIONS
  Cytological model is a more sensitive technique to evaluate toxin producing abilities of non-O1/non-O139 V. cholerae strains and is appropriate for use.
authors: N R
authors: NR
authors: Telesmanich
authors: M V
authors: MV
authors: Akulova
authors: Iu M
authors: IuM
authors: Lomov
authors: L P
authors: LP
authors: Alekseeva
authors: V D
authors: VD
authors: Kruglikova
authors: V V
authors: VV
authors: Agafonova
authors: O A
authors: OA
authors: Tatarenko
authors: V V
authors: VV
authors: Evdokimova