title: N-glycoproteomics in plants: perspectives and challenges.
abstract: In eukaryotes, proteins that are secreted into the ER are mostly modified by N-glycans on consensus NxS/T sites. The N-linked glycan subsequently undergoes varying degrees of processing by enzymes which are spatially distributed over the ER and the Golgi apparatus. The post-ER N-glycan processing to complex glycans differs between animals and plants, with consequences for N-glycan and glycopeptide isolation and characterization of plant glycoproteins. Here we describe some recent developments in plant glycoproteomics and illustrate how general and plant specific technologies may be used to address different important biological questions.
authors: Plant Research International, Wageningen University and Research Centre, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands.
authors: Wei
authors: W
authors: Song
authors: Maurice G L
authors: MG
authors: Henquet
authors: Remco A
authors: RA
authors: Mentink
authors: Aalt Jan
authors: AJ
authors: van Dijk
authors: Jan H G
authors: JH
authors: Cordewener
authors: Dirk
authors: D
authors: Bosch
authors: Antoine H P
authors: AH
authors: America
authors: Alexander R
authors: AR
authors: van der Krol