title: Large-scale transcriptome analysis in chickpea (Cicer arietinum L.), an orphan legume crop of the semi-arid tropics of Asia and Africa.
abstract: Chickpea (Cicer arietinum L.) is an important legume crop in the semi-arid regions of Asia and Africa. Gains in crop productivity have been low however, particularly because of biotic and abiotic stresses. To help enhance crop productivity using molecular breeding techniques, next generation sequencing technologies such as Roche/454 and Illumina/Solexa were used to determine the sequence of most gene transcripts and to identify drought-responsive genes and gene-based molecular markers. A total of 103,215 tentative unique sequences (TUSs) have been produced from 435,018 Roche/454 reads and 21,491 Sanger expressed sequence tags (ESTs). Putative functions were determined for 49,437 (47.8%) of the TUSs, and gene ontology assignments were determined for 20,634 (41.7%) of the TUSs. Comparison of the chickpea TUSs with the Medicago truncatula genome assembly (Mt 3.5.1 build) resulted in 42,141 aligned TUSs with putative gene structures (including 39,281 predicted intron/splice junctions). Alignment of ∼37 million Illumina/Solexa tags generated from drought-challenged root tissues of two chickpea genotypes against the TUSs identified 44,639 differentially expressed TUSs. The TUSs were also used to identify a diverse set of markers, including 728 simple sequence repeats (SSRs), 495 single nucleotide polymorphisms (SNPs), 387 conserved orthologous sequence (COS) markers, and 2088 intron-spanning region (ISR) markers. This resource will be useful for basic and applied research for genome analysis and crop improvement in chickpea.
authors: International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, India.
authors: Pavana J
authors: PJ
authors: Hiremath
authors: Andrew
authors: A
authors: Farmer
authors: Steven B
authors: SB
authors: Cannon
authors: Jimmy
authors: J
authors: Woodward
authors: Himabindu
authors: H
authors: Kudapa
authors: Reetu
authors: R
authors: Tuteja
authors: Ashish
authors: A
authors: Kumar
authors: Amindala
authors: A
authors: Bhanuprakash
authors: Benjamin
authors: B
authors: Mulaosmanovic
authors: Neha
authors: N
authors: Gujaria
authors: Laxmanan
authors: L
authors: Krishnamurthy
authors: Pooran M
authors: PM
authors: Gaur
authors: Polavarapu B
authors: PB
authors: Kavikishor
authors: Trushar
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authors: Shah
authors: Ramamurthy
authors: R
authors: Srinivasan
authors: Marc
authors: M
authors: Lohse
authors: Yongli
authors: Y
authors: Xiao
authors: Christopher D
authors: CD
authors: Town
authors: Douglas R
authors: DR
authors: Cook
authors: Gregory D
authors: GD
authors: May
authors: Rajeev K
authors: RK
authors: Varshney
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