title: A reverse transcription-free real-time PCR assay for rapid miRNAs quantification based on effects of base stacking.
abstract: A rapid and reverse transcription-free real-time PCR microRNA assay was developed based on effects of base stacking. This microRNA assay has been shown to be highly specific to homogenous miRNAs, and the procedure can be completed within 30 min starting from total RNA.
authors: Suzhou Institute of Nano-tech and Nano-bionics, Chinese Academy of Sciences, 398 Ruoshui Road, Dushu Lake Higher Education Town, Suzhou Industrial Park, Suzhou 215123, P. R. China.
authors: Zhuoxuan
authors: Z
authors: Lu
authors: Demin
authors: D
authors: Duan
authors: Rong
authors: R
authors: Cao
authors: Liming
authors: L
authors: Zhang
authors: Kexiao
authors: K
authors: Zheng
authors: Jiong
authors: J
authors: Li