title: Alphacoronaviruses in New World bats: prevalence, persistence, phylogeny, and potential for interaction with humans.
abstract: Bats are reservoirs for many different coronaviruses (CoVs) as well as many other important zoonotic viruses. We sampled feces and/or anal swabs of 1,044 insectivorous bats of 2 families and 17 species from 21 different locations within Colorado from 2007 to 2009. We detected alphacoronavirus RNA in bats of 4 species: big brown bats (Eptesicus fuscus), 10% prevalence; long-legged bats (Myotis volans), 8% prevalence; little brown bats (Myotis lucifugus), 3% prevalence; and western long-eared bats (Myotis evotis), 2% prevalence. Overall, juvenile bats were twice as likely to be positive for CoV RNA as adult bats. At two of the rural sampling sites, CoV RNAs were detected in big brown and long-legged bats during the three sequential summers of this study. CoV RNA was detected in big brown bats in all five of the urban maternity roosts sampled throughout each of the periods tested. Individually tagged big brown bats that were positive for CoV RNA and later sampled again all became CoV RNA negative. Nucleotide sequences in the RdRp gene fell into 3 main clusters, all distinct from those of Old World bats. Similar nucleotide sequences were found in amplicons from gene 1b and the spike gene in both a big-brown and a long-legged bat, indicating that a CoV may be capable of infecting bats of different genera. These data suggest that ongoing evolution of CoVs in bats creates the possibility of a continued threat for emergence into hosts of other species. Alphacoronavirus RNA was detected at a high prevalence in big brown bats in roosts in close proximity to human habitations (10%) and known to have direct contact with people (19%), suggesting that significant potential opportunities exist for cross-species transmission of these viruses. Further CoV surveillance studies in bats throughout the Americas are warranted.
authors: Department of Pediatrics, University of Colorado School of Medicine, Aurora, Colorado, United States of America.
authors: Christina
authors: C
authors: Osborne
authors: Paul M
authors: PM
authors: Cryan
authors: Thomas J
authors: TJ
authors: O'Shea
authors: Lauren M
authors: LM
authors: Oko
authors: Christina
authors: C
authors: Ndaluka
authors: Charles H
authors: CH
authors: Calisher
authors: Andrew D
authors: AD
authors: Berglund
authors: Mead L
authors: ML
authors: Klavetter
authors: Richard A
authors: RA
authors: Bowen
authors: Kathryn V
authors: KV
authors: Holmes
authors: Samuel R
authors: SR
authors: Dominguez
references: J Virol. 2010 Dec;84(24):13004-18
references: 20926577
references: J Gen Virol. 2009 Aug;90(Pt 8):2015-2022
references: 19357225
references: BMC Infect Dis. 2009 Sep 28;9:159
references: 19785757
references: Yale J Biol Med. 1974 Dec;47(4):234-51
references: 4617423
references: Emerg Infect Dis. 2009 Mar;15(3):482-5
references: 19239771
references: J Virol. 2005 Feb;79(4):2001-9
references: 15681402
references: Lancet. 1999 Oct 9;354(9186):1257-9
references: 10520635
references: Emerg Infect Dis. 2009 Sep;15(9):1377-84
references: 19788804
references: Science. 2005 Oct 28;310(5748):676-9
references: 16195424
references: Emerg Infect Dis. 2000 Jul-Aug;6(4):363-9
references: 10970147
references: J Mammal. 2016 Jun 9;97(3):663-688
references: 29692469
references: Science. 2005 Jan 28;307(5709):580-4
references: 15681385
references: Proc Natl Acad Sci U S A. 2007 Jul 3;104(27):11424-9
references: 17592121
references: Emerg Infect Dis. 2007 Sep;13(9):1295-300
references: 18252098
references: Emerg Infect Dis. 2010 Aug;16(8):1217-23
references: 20678314
references: Emerg Infect Dis. 2008 Apr;14(4):626-31
references: 18400147
references: J Virol. 2010 Jul;84(14):6955-65
references: 20463061
references: Rev Med Virol. 2007 Mar-Apr;17(2):67-91
references: 17042030
references: Emerg Infect Dis. 1999 May-Jun;5(3):433-7
references: 10341181
references: J Gen Virol. 2006 Sep;87(Pt 9):2461-2466
references: 16894183
references: Med J Aust. 1995 Jun 19;162(12):642-5
references: 7603375
references: J Virol. 2010 Mar;84(6):2808-19
references: 20071579
references: Clin Microbiol Rev. 2006 Jul;19(3):531-45
references: 16847084
references: Biol Rev Camb Philos Soc. 2002 May;77(2):223-59
references: 12056748
references: PLoS One. 2008;3(11):e3803
references: 19030226
references: J Virol. 2007 Feb;81(4):1574-85
references: 17121802
references: Emerg Infect Dis. 2008 Dec;14(12):1890-3
references: 19046513
references: J Virol. 2010 Nov;84(21):11385-94
references: 20702646
references: Emerg Infect Dis. 1995 Jan-Mar;1(1):31-3
references: 8903153
references: J Virol. 2010 Nov;84(21):11336-49
references: 20686038
references: J Virol. 2007 Apr;81(8):4012-20
references: 17267506
references: PLoS Pathog. 2009 Jul;5(7):e1000536
references: 19649327
references: J Am Vet Med Assoc. 2003 Mar 1;222(5):633-9
references: 12619845
references: Science. 2010 Aug 6;329(5992):676-9
references: 20689015
references: Clin Infect Dis. 2007 Mar 1;44(5):711-7
references: 17278066
references: Proc Natl Acad Sci U S A. 2005 Sep 27;102(39):14040-5
references: 16169905
references: J Virol. 2006 Aug;80(15):7481-90
references: 16840328
references: Exp Biol Med (Maywood). 2009 Oct;234(10):1117-27
references: 19546349
references: Mol Biol Evol. 2007 Aug;24(8):1596-9
references: 17488738
references: Emerg Infect Dis. 2007 Sep;13(9):1367-70
references: 18252111
references: J Virol. 1988 May;62(5):1810-3
references: 2833625