title: Proteome analysis of Thermococcus onnurineus NA1 reveals the expression of hydrogen gene cluster under carboxydotrophic growth.
abstract: Thermococcus onnurineus NA1 is a typical sulfur-reducing hyperthermophilic archaeon. Genome sequence analysis has shown that T. onnurineus NA1 retains the metabolic pathways necessary not only for organotrophic, but also for carboxydotrophic, growth. T. onnurineus NA1 carboxydotrophic growth may result in hydrogen production, as this archaeon produces hydrogen during oxidizing formate. In this study, we profiled the proteome of T. onnurineus NA1 cultured under carboxydotrophic conditions using CO as an electron donor by the SDS-PAGE/LC-MS/MS method. A total of 1395 proteins were identified by two independent proteomic analyses, which corresponds to ~71% of the total predicted open reading frames. To our knowledge, this level of identification coverage exceeds those of other global proteome profiling studies in Archaea. Furthermore, the biological functions of the identified proteins were predicted and cognate enzymes were mapped to the appropriate metabolic pathways. More than 90% of the genes belonging to hydrogenase gene clusters such as Mbx, Sulf-I, Mbh, Hyg4-I, Hyg4-II, and Hyf4-III were expressed during CO culture. This means that hydrogenases induced under carboxydotrophic conditions surpass those induced under an organotrophic condition (yeast extract-peptone-sulfur). Our data suggest that hydrogen production is caused by the expression and functional assembly of T. onnurineus NA1 hydrogenase genes during culture in the presence of CO.
authors: Division of Life Science, Korea Basic Science Institute, Daejeon 305-333, Republic of Korea.
authors: Sung-Ho
authors: SH
authors: Yun
authors: Sang Oh
authors: SO
authors: Kwon
authors: Gun Wook
authors: GW
authors: Park
authors: Jin Young
authors: JY
authors: Kim
authors: Sung Gyun
authors: SG
authors: Kang
authors: Jung-Hyun
authors: JH
authors: Lee
authors: Young-Ho
authors: YH
authors: Chung
authors: Soohyun
authors: S
authors: Kim
authors: Jong-Soon
authors: JS
authors: Choi
authors: Seung Il
authors: SI
authors: Kim