title: The polyketide MPBD initiates the SDF-1 signaling cascade that coordinates terminal differentiation in Dictyostelium.
abstract: Dictyostelium uses a wide array of chemical signals to coordinate differentiation as it switches from a unicellular to a multicellular organism. MPBD, the product of the polyketide synthase encoded by stlA, regulates stalk and spore differentiation by rapidly stimulating the release of the phosphopeptide SDF-1. By analyzing specific mutants affected in MPBD or SDF-1 production, we delineated a signal transduction cascade through the membrane receptor CrlA coupled to Gα1, leading to the inhibition of GskA so that the precursor of SDF-1 is released. It is then processed by the extracellular protease of TagB on prestalk cells. SDF-1 apparently acts through the adenylyl cyclase ACG to activate the cyclic AMP (cAMP)-dependent protein kinase A (PKA) and trigger the production of more SDF-1. This signaling cascade shows similarities to the SDF-2 signaling pathway, which acts later to induce rapid spore encapsulation.
authors: Division of Biological Sciences, University of California-San Diego, La Jolla, CA 92093-0368, USA.
authors: Christophe
authors: C
authors: Anjard
authors: Yongxuan
authors: Y
authors: Su
authors: William F
authors: WF
authors: Loomis
references: J Cell Biol. 2010 Feb 22;188(4):537-46
references: 20156962
references: Proc Natl Acad Sci U S A. 2005 May 24;102(21):7607-11
references: 15897458
references: Differentiation. 1997 Oct;62(1):43-9
references: 9373946
references: Mol Cell. 2000 Dec;6(6):1509-14
references: 11163223
references: Trends Biochem Sci. 2001 Oct;26(10):582-4
references: 11590001
references: Mol Cell Biol. 1999 Jul;19(7):4750-6
references: 10373524
references: J Biol Chem. 1998 Apr 3;273(14):8161-8
references: 9525920
references: Development. 1999 Jan;126(2):325-33
references: 9847246
references: Cell. 2007 Aug 10;130(3):524-34
references: 17655921
references: Development. 1994 Dec;120(12):3549-61
references: 7821221
references: Dev Biol. 1988 Mar;126(1):108-14
references: 2830156
references: Nat Chem Biol. 2006 Sep;2(9):494-502
references: 16906151
references: Development. 2006 Jun;133(11):2253-61
references: 16672332
references: Biochim Biophys Acta. 2006 May;1760(5):754-61
references: 16545913
references: J Cell Biol. 2010 Feb 22;188(4):527-36
references: 20156967
references: Genome Biol. 2010;11(3):R35
references: 20236529
references: Development. 1998 Oct;125(20):4067-75
references: 9735367
references: Differentiation. 2003 Jan;71(1):51-61
references: 12558603
references: Genes Dev. 1995 May 1;9(9):1111-22
references: 7744252
references: Science. 2004 May 7;304(5672):875-8
references: 15131307
references: J Biol Chem. 2010 Jul 9;285(28):21359-65
references: 20452969
references: J Gen Microbiol. 1954 Feb;10(1):110-20
references: 13130832
references: Curr Mol Med. 2003 Aug;3(5):472-82
references: 12943000
references: Cell. 1995 Jan 13;80(1):139-48
references: 7813009
references: Development. 2008 Mar;135(5):819-27
references: 18216168
references: Exp Cell Res. 1993 Jul;207(1):107-14
references: 8319762
references: Biochem Biophys Res Commun. 2001 Jan 26;280(3):720-5
references: 11162580
references: Dev Biol. 2004 Jan 15;265(2):433-45
references: 14732403
references: Development. 2009 Mar;136(5):803-12
references: 19176583
references: FEBS Lett. 2004 Oct 22;576(3):287-90
references: 15498549
references: Cell. 1999 Nov 12;99(4):399-408
references: 10571182
references: Development. 2004 Sep;131(18):4555-65
references: 15342480
references: J Biol Chem. 2008 Apr 25;283(17):11348-54
references: 18252726
references: Dev Biol. 2008 May 15;317(2):444-53
references: 18402932
references: Development. 1992 Jul;115(3):785-90
references: 1330484
references: FEMS Microbiol Lett. 2011 Jun;319(1):82-7
references: 21438914
references: Dis Model Mech. 2009 May-Jun;2(5-6):306-12
references: 19383941
references: Eukaryot Cell. 2010 Jul;9(7):1009-17
references: 20472692
references: Eur J Biochem. 1983 Oct 17;136(1):51-6
references: 6617660
references: Dev Biol. 1998 Jan 15;193(2):146-55
references: 9473320