title: A self assembled monolayer based microfluidic sensor for urea detection.
abstract: Urease (Urs) and glutamate dehydrogenase (GLDH) have been covalently co-immobilized onto a self-assembled monolayer (SAM) comprising of 10-carboxy-1-decanthiol (CDT) via EDC-NHS chemistry deposited onto one of the two patterned gold (Au) electrodes for estimation of urea using poly(dimethylsiloxane) based microfluidic channels (2 cm × 200 μm × 200 μm). The CDT/Au and Urs-GLDH/CDT/Au electrodes have been characterized using Fourier transform infrared (FTIR) spectroscopy, contact angle (CA), atomic force microscopy (AFM) and electrochemical cyclic voltammetry (CV) techniques. The electrochemical response measurement of a Urs-GLDH/CDT/Au bioelectrode obtained as a function of urea concentration using CV yield linearity as 10 to 100 mg dl(-1), detection limit as 9 mg dl(-1) and high sensitivity as 7.5 μA mM(-1) cm(-2).
authors: Department of Science and Technology Centre on Biomolecular Electronics, Biomedical Instrumentation Section, National Physical Laboratory, New Delhi 110012, India.
authors: Saurabh
authors: S
authors: Srivastava
authors: Pratima R
authors: PR
authors: Solanki
authors: Ajeet
authors: A
authors: Kaushik
authors: Md Azahar
authors: MA
authors: Ali
authors: Anchal
authors: A
authors: Srivastava
authors: B D
authors: BD
authors: Malhotra