title: Positional proteomics at the N-terminus as a means of proteome simplification.
abstract: One strategy to reduce complexity in proteome analysis is through rational reduction of the proteolytic peptides that constitute the analyte for mass spectrometric analysis. Methods for selective isolation of C- and N-terminal peptides have been developed. In this chapter, we outline the context and variety of methods for selective isolation of N-terminal peptides and detail one method based on negative selection through differential removal of internal peptides.
authors: Protein Function Group, Institute of Integrative Biology, University of Liverpool, Liverpool, L69 7ZJ, UK.
authors: Gemma R
authors: GR
authors: Davidson
authors: Stuart D
authors: SD
authors: Armstrong
authors: Robert J
authors: RJ
authors: Beynon