title: [Simultaneous determination of two saponnins in anemarrhenae rhizoma by HPLC-ELSD].
abstract: OBJECTIVE
  To establish an HPLC-ELSD method for determination of Anemarsaponin C and Anemarsaponin A III in Anemarrhenae Rhizoma.
  
  
  METHODS
  Kromasil C18 column(4.6 mm x 250 mm, 5 microm) was used as stationary phase. Mobile phase was methanol-water gradient with the flow rate of 1 mL x min(-1); the temperature of the drift tube and evaporation was 50 degrees C and 70 degrees C respectively. The gas pressure was 1.03 x 10(5) Pa.
  
  
  RESULTS
  There are good linearity in the range 0.310-3.10 microg of anemarsaponin C (lgA = 1.254 2lgM + 5.734 7, r = 0.999 5) and in the range 0.323-3.23 microg (lgA = 1.328 41gM + 5. 937, r = 0.999 6) of anemarsaponin A III. The average recovery of anemarsaponin C and anemarsaponin A III was 98.1% with RSD 2.1% and 97.3% with RSD 1.5% (n = 6) respectively.
  
  
  CONCLUSIONS
  The method is rapid and accurate. It is suitable for quality control of Anemarrhenae Rhizoma. The result of determination reveals that the quality of Anemarrhenae Rhizoma from different places of north China are of notable difference.
authors: Resource Biology and Modern Biotechnology Key Laboratory in Western China of Education Ministry, College of Life Science, Northwest University, Xi'an 710069, China.
authors: Qianliang
authors: Q
authors: Chen
authors: Xiaoming
authors: X
authors: Sun
authors: Wenquan
authors: W
authors: Wang
authors: Changhua
authors: C
authors: Ma