title: Analysis on the alterations of lens proteins by Vitex negundo in selenite cataract models.
abstract: OBJECTIVE
  Cataract is the leading cause of blindness and is associated with oxidative damage and protein modification in the lens. In the present study, we have employed proteomic and microscopic approaches to investigate the attenuation of selenite cataract by the flavonoids from Vitex negundo (FVN).
  
  
  METHODS
  To demonstrate this attenuation, Sprague-Dawley rat pups were divided into control (G I), selenite induced (G II), and selenite + FVN treated (G III). Cataract was induced by single subcutaneous injection of sodium selenite (4 mg/Kg bodyweight) on the 10th day and FVN (1 mg/Kg bodyweight) administered intraperitoneally from the 8th to the 15th day.
  
  
  RESULTS
  Our study indicated that chaperone property of α-crystallin and soluble protein levels were reduced in the selenite induced group. Post translational modifications identified by two dimensional-polyacrylamide gel electrophoresis (2D-PAGE) and immunoblot analysis revealed the loss of cytoskeletal proteins in selenite induced group. Damage of lenticular membrane and abnormal fiber structure were observed by electron microscopy.
  
  
  CONCLUSIONS
  The results of this study suggest that FVN modulated selenite induced cataractogensis in rat pups by preventing loss of chaperone property, various changes in lens proteins, and lens structure, further strengthening its protective role.
authors: Department of Biochemistry, University of Kerala, Kariavattom, Thiruvananthapuram, India.
authors: B N
authors: BN
authors: Rooban
authors: V
authors: V
authors: Sasikala
authors: V
authors: V
authors: Sahasranamam
authors: Annie
authors: A
authors: Abraham
references: Experientia. 1978 Feb 15;34(2):222-3
references: 624358
references: Exp Eye Res. 2005 May;80(5):709-25
references: 15862178
references: Mol Vis. 1997 Jul 23;3:8
references: 9238097
references: Mol Vis. 2007 Jul 12;13:1121-9
references: 17653057
references: J Biol Chem. 2000 Feb 18;275(7):4565-70
references: 10671481
references: Exp Eye Res. 2002 Jun;74(6):689-702
references: 12126943
references: Exp Eye Res. 1997 Mar;64(3):387-95
references: 9196390
references: Exp Eye Res. 1985 Feb;40(2):213-22
references: 3979462
references: Mol Vis. 2005 Jul 26;11:561-8
references: 16088325
references: J Biol Chem. 1993 Jan 25;268(3):1937-40
references: 8420967
references: Exp Eye Res. 2008 Feb;86(2):251-9
references: 18068705
references: FEBS Lett. 1993 Jun 21;324(3):265-70
references: 8405363
references: Ophthalmologica. 2000 Jan-Feb;214(1):78-85
references: 10657746
references: Invest Ophthalmol Vis Sci. 1994 Mar;35(3):785-93
references: 8125740
references: Phytother Res. 2001 Sep;15(6):519-23
references: 11536382
references: Invest Ophthalmol Vis Sci. 1982 Jul;23(1):1-13
references: 7085211
references: J Ethnopharmacol. 2003 Aug;87(2-3):199-206
references: 12860308
references: Invest Ophthalmol Vis Sci. 1984 Jun;25(6):751-7
references: 6724845
references: Jpn J Ophthalmol. 2000 Nov 1;44(6):690
references: 11094191
references: J Cataract Refract Surg. 2007 Jan;33(1):104-7
references: 17189803
references: Curr Eye Res. 2005 Jul;30(7):583-91
references: 16020293
references: Surv Ophthalmol. 2008 Jul-Aug;53(4):359-67
references: 18572053
references: Invest Ophthalmol Vis Sci. 1984 Nov;25(11):1275-83
references: 6386740
references: Mol Vis. 2003 Dec 08;9:644-56
references: 14685148
references: Exp Eye Res. 1995 Mar;60(3):245-55
references: 7789405
references: Exp Eye Res. 2004 Nov;79(5):595-612
references: 15500819
references: J Neurosci. 2001 Nov 1;21(21):8315-8
references: 11606617
references: Exp Eye Res. 2009 Mar;88(3):575-82
references: 19094987
references: Ophthalmic Res. 1983;15(3):146-59
references: 6634051
references: J Biol Chem. 1993 Sep 5;268(25):18844-9
references: 8395520
references: Mol Vis. 2008 Jul 10;14:1282-7
references: 18618007
references: J Proteome Res. 2007 Oct;6(10):3935-43
references: 17824632
references: Mol Cell Proteomics. 2002 May;1(5):357-65
references: 12118077
references: Eye (Lond). 2003 Jul;17(5):637-45
references: 12855974
references: Curr Eye Res. 1992 Dec;11(12):1147-60
references: 1337028
references: Invest Ophthalmol Vis Sci. 1996 Jun;37(7):1396-410
references: 8641842
references: Mol Vis. 2006 Jun 22;12:712-24
references: 16807531
references: Nutrition. 2003 Sep;19(9):794-9
references: 12921892
references: Biol Trace Elem Res. 2010 Dec;138(1-3):282-92
references: 20174976
references: Invest Ophthalmol Vis Sci. 2003 May;44(5):2067-75
references: 12714645
references: Curr Eye Res. 1998 Nov;17(11):1037-43
references: 9846621
references: Exp Cell Res. 2005 Apr 15;305(1):1-9
references: 15777782
references: Proteomics. 2006 Jan;6(2):667-76
references: 16342238
references: J Med Food. 2004 Fall;7(3):343-8
references: 15383230
references: Jpn J Ophthalmol. 2000 Nov 1;44(6):693-694
references: 11094199
references: J Biol Chem. 1951 Nov;193(1):265-75
references: 14907713
references: Phytother Res. 2006 Dec;20(12):1091-5
references: 17009203