title: Isolation of endothelial cells and vascular smooth muscle cells from internal mammary artery tissue.
abstract: Analyses of vascular smooth muscle cell and endothelial cell function through tissue culture techniques are often employed to investigate the underlying mechanisms regulating cardiovascular disease. As diseases such as diabetes mellitus and chronic kidney disease increase a patient's risk of cardiovascular disease, the development of methods for examining the effects of these diseases on vascular smooth muscle cells and endothelial cells is needed. Commercial sources of endothelial cells and vascular smooth muscle cells generally provide minimal donor information and are in limited supply. This study was designed to determine if vascular smooth muscle cells and endothelial cells could be isolated from human internal mammary arteries obtained from donors undergoing coronary artery bypass graft surgery. As coronary artery bypass graft surgery is a commonly performed procedure, this method would provide a new source for these cells that when combined with the donor's medical history will greatly enhance our studies of the effects of complicating diseases on vascular biology. Internal mammary artery tissue was obtained from patients undergoing coronary artery bypass graft surgery. Through a simple method employing two separate tissue digestions, vascular smooth muscle cells and endothelial cells were isolated and characterized. The isolated vascular smooth muscle cells and endothelial cells exhibited the expected morphology and were able to be passaged for further analysis. The vascular smooth muscle cells exhibited positive staining for α-smooth muscle actin and the endothelial cells exhibited positive staining for CD31. The overall purity of the isolations was > 95%. This method allows for the isolation of endothelial cells and vascular smooth muscle cells from internal mammary arteries, providing a new tool for investigations into the interplay of vascular diseases and complicating diseases such as diabetes and kidney disease.
authors: Stephanie C
authors: SC
authors: Moss
authors: Michael
authors: M
authors: Bates
authors: Patrick E
authors: PE
authors: Parrino
authors: T Cooper
authors: TC
authors: Woods
references: Annu Rev Med. 2004;55:169-78
references: 14746515
references: N Engl J Med. 1976 Aug 19;295(8):420-5
references: 778621
references: Circulation. 1995 Jun 1;91(11):2793-801
references: 7758186
references: Heart. 2006 May;92(5):650-7
references: 16251229
references: Curr Atheroscler Rep. 2006 May;8(3):240-4
references: 16640961
references: N Engl J Med. 1999 Jan 14;340(2):115-26
references: 9887164
references: N Engl J Med. 1998 Jul 23;339(4):229-34
references: 9673301
references: Ann N Y Acad Sci. 1990;598:143-58
references: 2123377
references: Arterioscler Thromb Vasc Biol. 2003 Sep 1;23(9):1510-20
references: 12907463
references: Transplant Proc. 1993 Apr;25(2):2041-3
references: 8470266
references: Curr Vasc Pharmacol. 2003 Jun;1(2):111-21
references: 15320838
references: Arterioscler Thromb Vasc Biol. 1998 May;18(5):677-85
references: 9598824
references: N Engl J Med. 1986 Feb 20;314(8):488-500
references: 3511384
references: Nature. 1993 Apr 29;362(6423):801-9
references: 8479518
references: J Atheroscler Thromb. 2005;12(3):138-42
references: 16020913
references: Am J Kidney Dis. 1998 Nov;32(5 Suppl 3):S112-9
references: 9820470
references: Annu Rev Physiol. 1995;57:791-804
references: 7778883
references: Lab Invest. 1975 Mar;32(3):339-51
references: 1123913
references: Cell Tissue Res. 1995 Sep;281(3):421-33
references: 7553764
references: N Engl J Med. 1976 Aug 12;295(7):369-77
references: 819830
references: Circulation. 2007 Feb 6;115(5):e69-171
references: 17194875