title: Airway inflammation and central respiratory control: results from in vivo and in vitro neonatal rat.
abstract: In infants, respiratory infection elicits tachypnea. To begin to evaluate the role of brainstem cytokine expression in modulation of breathing pattern changes, we compared the pattern generated after endotracheal instillation of lipopolysaccharide (LPS) in in vivo rat pups to local pro-inflammatory cytokine injection in the nucleus tractus solitarius (nTS) in an in vitro en bloc brainstem spinal cord preparation. We hypothesized that both challenges would elicit similar changes in patterning of respiration. In anesthetized, spontaneously breathing rat pups, lipopolysaccharide (LPS) or saline was instilled in the airway of urethane-anesthetized rats (postnatal days 10-11). We recorded diaphragm EMG over the subsequent 2h and saw a 20-30% decrease in interburst interval (Te) at 20-80min post-injection in LPS-instilled animals with no significant change in Ti. In contrast, IL-1β injections into the nTS of en bloc in vitro brainstem-spinal cord preparations from 0 to 5 day-old pups maintained Ti and caused an increase in Te as early as 20min later, decreasing frequency for 80-120min after injection. Our results suggest that the neonatal respiratory response to the cytokine IL-1β mediated inflammatory response depends on the site of the inflammatory stimulus and that the direct effect of IL-1β in the nTS is to slow rather than increase rate.
authors: Department of Pediatrics, Case Western Reserve University, Cleveland, OH 44106, United States.
authors: Kenneth
authors: K
authors: Gresham
authors: Brooke
authors: B
authors: Boyer
authors: Catherine
authors: C
authors: Mayer
authors: Ryan
authors: R
authors: Foglyano
authors: Richard
authors: R
authors: Martin
authors: Christopher G
authors: CG
authors: Wilson
references: Annu Rev Physiol. 2006;68:279-305
references: 16460274
references: Scand J Immunol. 2011 Jan;73(1):53-8
references: 21129003
references: Glia. 2007 Apr 1;55(5):453-62
references: 17203472
references: Pediatr Infect Dis J. 1998 Jul;17(7):593-8
references: 9686724
references: JAMA. 2003 Nov 26;290(20):2677-84
references: 14645309
references: Brain Res. 2004 Oct 15;1023(2):264-71
references: 15374752
references: Eur J Neurosci. 2008 Dec;28(12):2499-510
references: 19087175
references: J Physiol. 1997 Oct 15;504 ( Pt 2):453-66
references: 9365917
references: J Neurosci Methods. 1987 Oct;21(2-4):321-33
references: 2890797
references: J Physiol. 2002 Mar 1;539(Pt 2):433-44
references: 11882676
references: Biochem Soc Trans. 2007 Nov;35(Pt 5):1122-6
references: 17956293
references: J Physiol. 1984 Sep;354:173-83
references: 6148410
references: Eur J Neurosci. 2008 Jun;27(12):3132-50
references: 18598258
references: Arch Pediatr Adolesc Med. 2003 Jan;157(1):26-32
references: 12517191
references: J Physiol. 1990 Aug;427:261-80
references: 2213599
references: J Neurophysiol. 2005 Feb;93(2):929-41
references: 15653788
references: Pediatrics. 1997 Nov;100(5):795-801
references: 9346978
references: J Appl Physiol (1985). 2001 Sep;91(3):1387-95
references: 11509540
references: Trends Neurosci. 1997 Jun;20(6):252-8
references: 9185306
references: J Gen Physiol. 2010 Feb;135(2):135-47
references: 20100893
references: Am J Physiol Renal Physiol. 2007 Oct;293(4):F1299-307
references: 17686952
references: Neurochem Int. 1999 Nov;35(5):345-55
references: 10517695
references: J Neurosci. 2007 Oct 3;27(40):10695-702
references: 17913903
references: Proc Natl Acad Sci U S A. 2007 Jun 5;104(23):9894-9
references: 17535900
references: JAMA. 2000 Sep 20;284(11):1417-24
references: 10989405
references: Pediatrics. 2002 Oct;110(4):673-80
references: 12359779
references: Biochem Biophys Res Commun. 2007 Apr 20;355(4):1064-8
references: 17336926
references: Respir Physiol Neurobiol. 2007 Oct 15;159(1):85-101
references: 17656160
references: J Neurosci. 2003 Jan 1;23(1):297-302
references: 12514227
references: J Neurosci. 1998 Nov 15;18(22):9471-9
references: 9801384
references: Prog Neurobiol. 1995 Oct;47(3):157-233
references: 8719915
references: Trends Immunol. 2002 Mar;23(3):144-50
references: 11864843
references: JAMA. 1997 Jul 16;278(3):207-11
references: 9218666
references: J Comp Neurol. 1980 Sep 15;193(2):467-508
references: 7440778
references: Nature. 2002 Dec 19-26;420(6917):853-9
references: 12490958
references: Early Hum Dev. 2001 Oct;65(1):21-37
references: 11520626
references: J Pharmacol Sci. 2007 Jan;103(1):93-102
references: 17220592
references: J Comp Neurol. 1980 Sep 15;193(2):435-65
references: 7440777
references: Neuroscience. 2003;119(1):65-72
references: 12763069
references: Am J Physiol. 1997 Nov;273(5):R1657-62
references: 9374806
references: Proc Natl Acad Sci U S A. 1991 Mar 15;88(6):2297-301
references: 11607165
references: Adv Exp Med Biol. 2008;605:393-8
references: 18085306
references: Brain Res. 1994 Feb 21;637(1-2):10-4
references: 8180786
references: Traffic. 2010 Mar;11(3):405-17
references: 20028484
references: Respir Physiol Neurobiol. 2011 Sep 30;178(3):458-64
references: 21397055
references: J Perinatol. 2010 Sep;30(9):604-9
references: 20182438
references: Pediatr Res. 2003 Sep;54(3):326-31
references: 12761362
references: Dev Med Child Neurol. 2009 Apr;51(4):253-4
references: 19335562
references: Blood. 1991 Apr 15;77(8):1627-52
references: 1826616
references: J Physiol. 2005 Apr 15;564(Pt 2):437-50
references: 15705651
references: Brain Res. 2006 Nov 20;1120(1):64-73
references: 17022949
references: Arch Physiol Biochem. 1999 Apr;107(2):97-112
references: 10650342