title: Intrathecal transplantation of stem cells by lumbar puncture for thoracic spinal cord injury in the rat.
abstract: METHODS
  Experimental investigation of intrathecal transplantation of stem cells by lumbar puncture (LP) in a rat model that simulates human thoracic spinal cord injury (SCI).
  
  
  OBJECTIVE
  To examine the distribution and phenotype of spinal cord-derived neural stem/progenitor cells (NSPCs) and bone marrow-derived mesenchymal stromal cells (BMSCs) following LP transplantation in SCI rats.
  
  
  METHODS
  Toronto Western Research Institute, Toronto, Ontario, Canada.
  
  
  METHODS
  NSPCs or BMSCs were transplanted via LP at level L3-5 1 week after compression SCI at T8. Rats were killed at 3, 17 and 27 days after LP transplantation and the relative distribution of cells at C4, T8 and L3-5 was quantitated. The phenotype of the NSPC and BMSC was assessed with immunocytochemistry in vitro and following LP transplantation.
  
  
  RESULTS
  By 4 weeks, more NSPC migrated to the lesion site relative to BMSC and uninjured animals. However, there was no preferential homing of either of these types of cells into the parenchyma of the injury site, and most of the transplanted cells remained in the intrathecal space. In vitro, spinal cord-derived NSPC proliferated and expressed nestin, but after LP transplantation, NSPC became post-mitotic and primarily expressed oligodendrocyte markers. In contrast, BMSC did not express any neural antigens in vivo.
  
  
  CONCLUSIONS
  LP is a minimally invasive method of cell transplantation that produces wide dissemination of cells in the subarachnoid space of the spinal cord. This is the first study to report and quantify the phenotype and spatial distribution of LP transplanted NSPC and BMSC in the intact and injured spinal cord.
authors: Toronto Western Research Institute and Krembil Neuroscience Centre, Toronto Western Hospital, Toronto, 399 Bathurst Street, Ontario, Canada. amothe@uhnres.utoronto.ca
authors: A J
authors: AJ
authors: Mothe
authors: G
authors: G
authors: Bozkurt
authors: J
authors: J
authors: Catapano
authors: J
authors: J
authors: Zabojova
authors: X
authors: X
authors: Wang
authors: A
authors: A
authors: Keating
authors: C H
authors: CH
authors: Tator