title: Molecular cloning, expression, sequence analyses of dust mite allergen Der f 6 and its IgE-binding reactivity with mite allergic asthma patients in southeast China.
abstract: We report the cloning and molecular characterization of a full-length cDNA encoding house dust mite allergen, Der f 6 from D. farinae isolated in China. The full-length Der f 6 cDNA was obtained with 840 nucleotides long. Nucleotide sequencing analyses showed a total of 36 mutations in five Der f 6 cDNA clones, corresponding to 23 incompatible amino acid residues. Recombinant Der f 6 (rDer f 6) protein was successfully expressed in and purified from E. coli BL21. Among 20 asthmatic patients, 45% was positive to rDer f 6 by ELISA. Bioinformatics analyses revealed that the mature Der f 6 was a hydrophobic and extracellular protein with chymotrypsin-like serine protease activity, its secondary structure was composed of alpha helix (7.69%), extended strand (34.62%), random coils (57.69%), and the similarity of Der f 6 to Blo t 6, Sui m 6, Der f 3 and Der f 9 was 64, 65, 35, and 38%, respectively.
authors: Department of Clinical Laboratory, Yancheng Health Vocational and Technical College, Yancheng, 224006 Jiangsu, People's Republic of China. ybcui1975@hotmail.com
authors: Yubao
authors: Y
authors: Cui
authors: Ying
authors: Y
authors: Zhou
authors: Weihong
authors: W
authors: Shi
authors: Guifang
authors: G
authors: Ma
authors: Li
authors: L
authors: Yang
authors: Yungang
authors: Y
authors: Wang
authors: Li
authors: L
authors: Li
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