title: Microsatellite primers in the planktonic diatom Pseudo-nitzschia multistriata (Bacillariophyceae).
abstract: OBJECTIVE
  Seven microsatellite loci were characterized for the toxic diatom Pseudo-nitzschia multistriata Takano (Takano) to investigate intraspecific variability and estimate population genetic structure over blooms, seasons, and sexual and vegetative reproduction.
  
  
  RESULTS
  Selected microsatellites consisted of di- and trinucleotide repeats in the core region, and showed four to twelve alleles per locus in strains of P. multistriata collected in the Gulf of Naples (Italy). Primer pairs were species-specific since they positively amplified against conspecific strains from Portugal and Spain but failed to generate PCR products from the diatoms Pseudo-nitzschia pseudodelicatissima (Hasle) Hasle and Leptocylindrus minimum Gran.
  
  
  CONCLUSIONS
  The seven selected microsatellite markers will be useful in studying population dynamics of Pseudo-nitzschia multistriata in space and time.
authors: Stazione Zoologica Anton Dohrn 80121 Naples, Italy. sylvie.tesson@szn.it
authors: Sylvie V M
authors: SV
authors: Tesson
authors: Marco
authors: M
authors: Borra
authors: Wiebe H C F
authors: WH
authors: Kooistra
authors: Gabriele
authors: G
authors: Procaccini