title: Proteome profiling of wild type and lumican-deficient mouse corneas.
abstract: To elucidate how the deficiency of a major corneal proteoglycan, lumican, affects corneal homeostasis, we used mass spectrometry to derive the proteome profile of the lumican-deficient and the heterozygous mouse corneas and compared these to the wild type corneal proteome. 2108 proteins were quantified in the mouse cornea. Selected proteins and transcripts were investigated by Western blot and quantitative RT-PCR, respectively. We observed major changes in the composition of the stromal extracellular matrix (ECM) proteins in the lumican-deficient mice. Lumican deficiency altered cellular proteins in the stroma and the corneal epithelium. The ECM changes included increases in fibril forming collagen type I, Collagen type VI, fibromodulin, perlecan, laminin β₂, collagen type IV, nidogen/entactin and anchoring collagen type VII in the Lum⁺/⁻ and the Lum⁻/⁻ mouse corneas, while the stromal proteoglycans decorin, biglycan and keratocan were decreased in the Lum⁻/⁻( corneas. Cellular protein changes included increases in alcohol dehydrogenase, superoxide dismutase and decreases in epithelial cytokeratins 8 and 14. We also detected proteins that are novel to the cornea. The proteomes will provide an insight into the lumican-deficient corneal phenotype of stromal thinning and loss of transparency and a better understanding of pathogenic changes in corneal and ocular dystrophies.
authors: Department of Medicine, The Johns Hopkins University School of Medicine, USA.
authors: Hanjuan
authors: H
authors: Shao
authors: Raghothama
authors: R
authors: Chaerkady
authors: Shoujun
authors: S
authors: Chen
authors: Sneha M
authors: SM
authors: Pinto
authors: Rakesh
authors: R
authors: Sharma
authors: Bernard
authors: B
authors: Delanghe
authors: David E
authors: DE
authors: Birk
authors: Akhilesh
authors: A
authors: Pandey
authors: Shukti
authors: S
authors: Chakravarti
references: Exp Eye Res. 2008 Dec;87(6):580-6
references: 18938159
references: Kidney Int. 1993 Jan;43(1):53-60
references: 8433569
references: Invest Ophthalmol Vis Sci. 2001 Jul;42(8):1750-6
references: 11431438
references: Cornea. 2001 Nov;20(8):853-8
references: 11685065
references: J Biol Chem. 2000 Jan 28;275(4):2607-12
references: 10644720
references: J Cell Biol. 2000 Nov 13;151(4):779-88
references: 11076963
references: J Biol Chem. 1990 Jun 25;265(18):10597-603
references: 2191952
references: Exp Eye Res. 2010 Sep;91(3):326-35
references: 20599432
references: Dev Dyn. 2010 Mar;239(3):844-54
references: 20108350
references: J Biol Chem. 2005 Jul 8;280(27):25541-7
references: 15849191
references: Invest Ophthalmol Vis Sci. 2010 Sep;51(9):4476-9
references: 20435602
references: FASEB J. 2000 Dec;14(15):2589-600
references: 11099478
references: Invest Ophthalmol Vis Sci. 2004 Feb;45(2):429-40
references: 14744882
references: Cont Lens Anterior Eye. 2003 Sep;26(3):139-46
references: 16303509
references: Cornea. 2009 Dec;28(10):1109-17
references: 19770725
references: Exp Eye Res. 1991 Jun;52(6):763-5
references: 1855550
references: Semin Cell Dev Biol. 2008 Apr;19(2):82-93
references: 17997336
references: Exp Eye Res. 1993 Jun;56(6):635-48
references: 8595806
references: Nat Genet. 1997 Mar;15(3):247-51
references: 9054935
references: Exp Eye Res. 2008 Sep;87(3):214-25
references: 18582462
references: Mol Vis. 2010 Oct 02;16:1949-57
references: 21031023
references: J Cell Biol. 1998 Jun 1;141(5):1277-86
references: 9606218
references: Glycoconj J. 2002 May-Jun;19(4-5):287-93
references: 12975607
references: Invest Ophthalmol Vis Sci. 2005 Jan;46(1):88-95
references: 15623759
references: Eye (Lond). 2010 Jun;24(6):1093-101
references: 20010793
references: Invest Ophthalmol Vis Sci. 2001 Oct;42(11):2496-504
references: 11581189
references: Exp Eye Res. 2004 May;78(5):957-71
references: 15051477
references: Invest Ophthalmol Vis Sci. 2003 Jun;44(6):2422-32
references: 12766039
references: Invest Ophthalmol Vis Sci. 2000 Oct;41(11):3365-73
references: 11006226
references: Semin Cell Dev Biol. 2008 Apr;19(2):100-12
references: 18077195
references: Development. 2007 Jul;134(13):2501-9
references: 17537792
references: Invest Ophthalmol Vis Sci. 2006 Jan;47(1):146-50
references: 16384956
references: Curr Eye Res. 2001 May;22(5):333-40
references: 11600933
references: Proteomics Clin Appl. 2007 Sep;1(9):1080-9
references: 21136759
references: Invest Ophthalmol Vis Sci. 2011 Feb 09;52(2):787-94
references: 20926822
references: Ann N Y Acad Sci. 1998 Oct 23;857:194-211
references: 9917842
references: Biochem Biophys Res Commun. 2009 May 15;382(4):776-9
references: 19324010
references: Invest Ophthalmol Vis Sci. 2003 Feb;44(2):548-57
references: 12556382
references: Exp Eye Res. 2010 Jun;90(6):655-63
references: 20223235
references: J Biol Chem. 2002 Sep 20;277(38):35532-40
references: 12089156
references: Mol Vis. 2007 Sep 11;13:1641-50
references: 17893666
references: J Immunol. 2007 Nov 15;179(10):6988-7000
references: 17982090
references: Mol Vis. 2009;15:706-12
references: 19365573