title: Simple cloning strategy using GFPuv gene as positive/negative indicator.
abstract: Because construction of expression vectors is the first requisite in the functional analysis of genes, development of simple cloning systems is a major requirement during the postgenomic era. In the current study, we developed cloning vectors for gain- or loss-of-function studies by using the GFPuv gene as a positive/negative indicator of cloning. These vectors allow us to easily detect correct clones and obtain expression vectors from a simple procedure by means of the combined use of the GFPuv gene and a type IIS restriction enzyme.
authors: Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, Isehara, Kanagawa 259-1193, Japan.
authors: Hiromi
authors: H
authors: Miura
authors: Hidetoshi
authors: H
authors: Inoko
authors: Ituro
authors: I
authors: Inoue
authors: Masafumi
authors: M
authors: Tanaka
authors: Masahiro
authors: M
authors: Sato
authors: Masato
authors: M
authors: Ohtsuka