title: Genome-specific SCAR markers help solve taxonomy issues: a case study with Sinapis arvensis (Brassiceae, Brassicaceae).
abstract: OBJECTIVE
  Traditional taxonomy and nomenclature of Brassiceae (Brassicaceae) species do not reflect their phylogeny. Revision of the species and generic limits supported by extensive molecular data seems crucial.
  
  
  RESULTS
  Genome-specific polymorphisms extracted from non-coding and coding sequences were used to develop 14 sequence characterized amplified region (SCAR) markers specific for the Brassica B genome. These SCARs were verified against 77 accessions of six U-triangle Brassica species and used to screen 23 accessions of seven wild Brassiceae species to test for their cross-species amplification. SCARs were found in all B-genome Brassica species and also in Sinapis arvensis.
  
  
  CONCLUSIONS
  SCAR markers can be employed for discerning B-genome chromosomes in Brassica species and S. arvensis to reliably identify B-genome species and their natural hybrids. The combined molecular evidence supports the suggestion to revise the generic limits of Brassica and Sinapis.
authors: Institute of Agricultural Biotechnology, 42 Timiryazevskaya ul., 127550 Moscow, Russia. artem.pankin@yahoo.com
authors: Artem A
authors: AA
authors: Pankin
authors: Emil E
authors: EE
authors: Khavkin