title: [Inhibitory effect of apatinib on HL-60 cell proliferation and its mechanism].
abstract: OBJECTIVE
  To investigate the effect of apatinib, a small-molecule vascular endothelial growth factor receptor-2 tyrosine kinase inhibitor, on the proliferation of human acute myeloid leukemia HL-60 cells and explore the possible mechanism.
  
  
  METHODS
  MTT assay was used to assess the cytotoxicity of apatinib in HL-60 cells. The apoptosis and cell cycle changes of the cells in response to apatinib treatment were analyzed by flow cytometry, and Western blotting was used to assay P-Akt and P-Erk1/2 expressions in the cells.
  
  
  RESULTS
  Apatinib significantly inhibited the proliferation of HL-60 cells in vitro with an IC(50) of 4.96∓0.32 µmol/L. Apatinib treatment significantly increased the apoptotic rate of the cells in a dose-dependent manner, but produced no significant effect on the cell cycle (P>0.05). Western blotting showed that the expressions of P-Akt and P-Erk1/2 decreased in HL-60 cells after a 48-h apatinib treatment.
  
  
  CONCLUSIONS
  Apatinib inhibits the proliferation of HL-60 cells by inducing cell apoptosis probably through the mechanism of inhibiting the expressions of the Akt/Erk1/2 signal transduction pathway.
authors: Department of Hematology, Yat-Sen University, Guangzhou 510060, China. Liangshu200900@126.com
authors: Shu
authors: S
authors: Liang
authors: Xiu-zhen
authors: XZ
authors: Tong
authors: Li-wu
authors: LW
authors: Fu