title: Intra-amniotic administration of exogenous pulmonary surfactant for improving in lung maturity of fetal rabbits with intrauterine infection caused by premature rupture of membranes.
abstract: This study was to investigate the effect of intra-amniotic administration of pulmonary surfactant (PS) on lung maturation in conditions of experimentally induced premature rupture of membranes (PROM) and intrauterine infection of rabbits. To establish animal (rabbit) models of intrauterine infection caused by PROM, E. coli was intrauterinely injected in 24-and 26-day pregnant animals. Twenty healthy pregnancy adult Japanese white rabbits were divided into three groups: the infection group (8 rabbits), infection group with intra-amniotic PS administration (8 rabbits) and healthy controls (4 rabbits). Ultrastructure changes in the lung were observed under an electron microscope 19.5 hours after intervention. The results showed that the lung infection levels of fetal rabbits in the infection group and the infection group with PS had no significant difference. Bacillus bodies and infiltrated inflammatory cells can be occasionally seen in the alveoli, bronchial lumen, and cytoplasm.The type II alveolar epithelial cells (AT-II) were decreased in the infection group than that in control group. Lamellar bodies showed vacuolation changes and different levels of apoptosis. In the infection group with PS, the count of AT-II did not show an obvious decrease. Lamellar bodies increased, and different secretion forms appeared. Also, there was little apoptosis and no obvious collagen fiber hyperplasia in antenatal administration of PS group. We believe that once PROM has happened, intrauterine infection and inflammation stimulated a cascade reaction on the fetal lung, leading to abnormal changes in the alveolar ultrastructure. Intra-amniotic administration of PS can improve the fetal lung ultrastructure and its maturity.
authors: Department of Neonatology & NICU, Bayi Children's Hospital Affiliated With General Hospital of Beijing Military Command, China. Liujingbj@live.cn
authors: Jing
authors: J
authors: Liu
authors: Jing
authors: J
authors: Wu
authors: Na
authors: N
authors: Yang
authors: ZhiChun
authors: Z
authors: Feng
references: Bosn J Basic Med Sci. 2009 Aug;9(3):225-8
references: 19754478
references: Am Fam Physician. 2006 Feb 15;73(4):659-64
references: 16506709
references: Clin Physiol Funct Imaging. 2003 Jul;23(4):199-203
references: 12914558
references: Med Wieku Rozwoj. 2003 Jul-Sep;7(3 Suppl 1):255-60
references: 15537270
references: J Perinatol. 2008 May;28 Suppl 1:S21-7
references: 18446173
references: Eur Respir J. 2008 Dec;32(6):1520-8
references: 18684851
references: N Engl J Med. 2000 May 18;342(20):1500-7
references: 10816189
references: J Perinat Med. 2004;32(4):354-8
references: 15346823
references: J Obstet Gynaecol Can. 2005 Jun;27(6):547-53
references: 16100631
references: J Obstet Gynaecol. 2006 Nov;26(8):740-3
references: 17130019
references: J Am Osteopath Assoc. 2004 Dec;104(12):537-42
references: 15653781
references: J Perinatol. 2005 Jun;25(6):403-7
references: 15843814
references: Am J Perinatol. 2008 Jan;25(1):69-73
references: 18075965
references: Antimicrob Agents Chemother. 2003 Jul;47(7):2125-30
references: 12821457
references: Pediatrics. 1996 Feb;97(2):210-5
references: 8584379
references: Am J Obstet Gynecol. 2001 Jul;185(1):173-7
references: 11483924
references: Chin Med J (Engl). 2004 Jan;117(1):120-4
references: 14733787
references: J Natl Med Assoc. 2006 Mar;98(3):340-4
references: 16573296
references: J Trop Pediatr. 2010 Feb;56(1):36-42
references: 19542192
references: Respir Res. 2001;2(1):27-32
references: 11686862
references: J Obstet Gynaecol. 2004 Apr;24(3):233-8
references: 15203614
references: Obstet Gynecol. 1992 Oct;80(4):604-8
references: 1407880
references: Anat Rec. 2001 Jun 1;263(2):118-26
references: 11360229