title: Luteolin induced G2 phase cell cycle arrest and apoptosis on non-small cell lung cancer cells.
abstract: In this study, we investigated the underlying molecular mechanism for the potent cell cycle inhibition and pro-apoptotic effect of luteolin (2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-4-chromenone) on human non-small-cell lung carcinoma cell line A549. MTT assay showed that luteolin had obvious cytotoxicity on A549 with IC(50) of 40.2 μM at 48 h. Pro-apoptotic effect of luteolin on A549 cells was demonstrated by Hoechst 33258 staining assay and annexin V-FITC/PI double staining analysis. A great quantity of apoptotic cells and increasing G2 phase cells were observed by flow cytometry. Western blotting assay revealed that luteolin activated JNK, increased Bax, promoted procaspase-9 cleavage and activated caspase-3 at last. Assay using TNFα, an active agent of NF-κB, showed that pretreatment of A549 cells with luteolin could inhibit TNFα induced trans-nuclear of NF-κB. In summary, luteolin displayed a significant cytotoxic effect through cell cycle arrest and apoptosis induction in A549 cells. Pro-apoptotic effect was implemented via activating JNK and inhibiting translocation of NF-κB (p65). These results suggested that luteolin might have therapeutic potential against NSCLC.
authors: School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, Jiangsu, China.
authors: Xueting
authors: X
authors: Cai
authors: Tingmei
authors: T
authors: Ye
authors: Chao
authors: C
authors: Liu
authors: Wuguang
authors: W
authors: Lu
authors: Min
authors: M
authors: Lu
authors: Juan
authors: J
authors: Zhang
authors: Min
authors: M
authors: Wang
authors: Peng
authors: P
authors: Cao