title: [Effect of removing dampness and promoting diuresis method on IgG, IgM and IL-1beta, IL-8 in serum of rats with autoimmunity induced by nucleus pulposus].
abstract: OBJECTIVE
  To explore the effects of removing dampness and promoting diuresis method on autoimmune and immuno-inflammatory response caused by nucleus pulposus of rats, in order to provide the basis for the treatment of lumbar disc herniation with Chinese medical immunotherapy.
  
  
  METHODS
  Forty male Wistar rats were divided into 4 groups randomly according to body weight layer:sham operation group (group A), model contrast group (group B), colchicine tablets group (group C), modified Qingyao decoction group (group D). There were 10 rats in each group. Nucleus pulposus of coccygeal vertebra was transplanted to the gluteal muscle by operation in groups B, C, D, which can lead to autoimmune and immuno-inflammatory response of rats; the rats of group A were only treated with sham operation. At the 3rd day after operation, the rats were fed through intragastric administration, the group A and B with distilled water (10 ml/kg), the group C and D respectively with suspension of colchicine tablets (10 ml/kg, 0.01 mg/ml) and water-decocted liquid of modified Qingyao decoction (10 ml/kg,1.035 g/ml), once a day and continuous medication for 18 days. All rats were killed at the 21th day after operation. The immunoglobulin G (IgG), immunoglobulin M (IgM) and interleukin-1beta (IL-1beta), interleukin-8 (IL-8) level in serum of different groups were detected by ELISA method. At the same time, surrounding tissues of the transplanted nucleus pulposus were observed by pathological section.
  
  
  RESULTS
  The level of IgG, IgM, IL-1beta, IL-8 in serum of group B was significantly higher than that of group A (P < 0.01), while the level of IgG, IgM, IL-1beta, IL-8 in serum of group C, D was significantly lower than that of group B (P < 0.05 or P < 0.01). Moreover, pathological section indicated that immuno-inflammatory response was hardly found in surgical site of group A, while local immuno-inflammatory response of surrounding tissues of the transplanted nucleus pulposus of group C and D was much lighter than that of group B.
  
  
  CONCLUSIONS
  Removing dampness and promoting diuresis method could inhibit autoimmune and immuno-inflammatory response caused by nucleus pulposus of rats.
authors: Department of the Second Spine, Wangjing Hospital of China Academy of Traditional Chinese Medicine Sciences, Beijing 100102, China.
authors: Li-Guo
authors: LG
authors: Zhu
authors: Xin
authors: X
authors: Chen
authors: Jie
authors: J
authors: Yu
authors: Jing-Hua
authors: JH
authors: Gao
authors: Shang-Quan
authors: SQ
authors: Wang
authors: Min-Shan
authors: MS
authors: Feng
authors: He
authors: H
authors: Yin