title: Synthesis and evaluation of dioleoyl glyceric acids showing antitrypsin activity.
abstract: Previously, Lešová et al. reported the isolation and identification of metabolite OR-1, showing antitrypsin activity, produced during fermentation by Penicillium funiculosum. The structure of OR-1 was a mixture of glyceric acid (GA), esterified with C(14)-C(18) fatty acids, and oleic acid (C18:1) as the most predominant fatty acid (Folia Microbiol. 46, 21-23, 2001). In this study, dioleoyl D-GA and dioleoyl L-GA were synthesized via diesterification with oleoyl chloride, and their antitrypsin activities were evaluated using both a disk diffusion method and spectral absorption measurements. The results show that both compounds and their equivalent mixtures possess antitrypsin activities; however, their IC(50) values (approximately 2 mM) are much higher than that of OR-1 (4.25 µM), suggesting that dioleoyl GA does not play a major role in the OR-1 antitrypsin activity.
authors: Research Institute for Innovations in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan. hiroshi.habe@aist.go.jp
authors: Hiroshi
authors: H
authors: Habe
authors: Tokuma
authors: T
authors: Fukuoka
authors: Shun
authors: S
authors: Sato
authors: Dai
authors: D
authors: Kitamoto
authors: Keiji
authors: K
authors: Sakaki