title: Effect of cerium lanthanide on Hela and MCF-7 cancer cell growth in the presence of transferring.
abstract: The anti-cancer activity of metal ions in the lanthanide group is being considered recently. It has been reported that cerium salts might stimulate the metabolism and therefore, produce anti-cancer effects. However, little is known about the effects of protein-cerium complex in controlling cancer cell growth. The aim of the present study was to elucidate the possible pathways for the cytotoxic effect of cerium in the presence of apo-transferrin on two cancer cell lines (Hela and MCF-7), that express transferrin receptors 3-4 fold higher than normal cells. The effect of different concentrations of cerium (0.1, 1, 10, 100 μM) in the presence and absence of transferrin for 48 h and 72 h incubation periods (37°C, 5% CO2 and 95% humidity) was studied using the MTT assay. The results showed that cerium has a cell-proliferation inhibitory activity which is significantly increased by transferrin protein. Compared with the direct treatment of cancer cells with cerium, the presence of transferrin assisted inhibition of cell proliferation by 20% and 40% in Hela and MCF-7 cells, respectively. Though apo-transferrin could lightly induce cell growth particularly in MCF-7 cells by itself, this phenomenon could not overcome the cerium-protein cell-proliferation inhibition activity. In conclusion, our results indicate that at a certain concentration, the cerium compounds could be possibly involved in the control of cell proliferation and inhibiting the growth of cancer cells.
authors: Department of Clinical Biochemistry and Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, I.R.Iran.
authors: A A
authors: AA
authors: Palizban
authors: H
authors: H
authors: Sadeghi-Aliabadi
authors: F
authors: F
authors: Abdollahpour
references: J Biol Inorg Chem. 2000 Dec;5(6):692-8
references: 11128996
references: J Cancer Res Clin Oncol. 1985;110(1):71-6
references: 2991295
references: Nat Rev Cancer. 2003 Jun;3(6):453-8
references: 12778135
references: J Immunol Methods. 1983 Dec 16;65(1-2):55-63
references: 6606682
references: Met Ions Biol Syst. 1998;35:585-631
references: 9444770
references: Drug Discov Today. 2005 Feb 15;10(4):267-73
references: 15708745
references: Zhonghua Yu Fang Yi Xue Za Zhi. 1997 Jul;31(4):228-30
references: 9812584
references: J Biol Chem. 1991 Oct 5;266(28):19006-12
references: 1918016
references: Cell. 2000 Jan 7;100(1):57-70
references: 10647931
references: Biochim Biophys Acta. 1963 Nov 8;77:383-93
references: 14089413
references: Lancet. 1980 Aug 23;2(8191):390-2
references: 6105517
references: Biomed Environ Sci. 2000 Dec;13(4):287-92
references: 11351862
references: J Leukoc Biol. 1996 Feb;59(2):189-95
references: 8603991
references: J Bioenerg Biomembr. 1987 Oct;19(5):535-49
references: 3693344
references: Biochemistry. 1971 Jul 20;10(15):2838-43
references: 5114527
references: J Clin Pathol. 1983 May;36(5):539-45
references: 6302135
references: J Exp Med. 1953 May;97(5):695-710
references: 13052828
references: Rev Physiol Biochem Pharmacol. 2005;153:101-11
references: 15674649
references: J Cell Physiol. 1984 Aug;120(2):225-32
references: 6746749
references: Int J Oncol. 2007 Jun;30(6):1545-51
references: 17487377
references: Cancer Lett. 2009 Dec 28;286(2):189-95
references: 19535202
references: Physiol Rev. 1987 Apr;67(2):520-82
references: 3550839
references: J Cell Physiol. 1993 Jul;156(1):212-7
references: 8314858