title: Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
abstract: To identify biomarker candidates associated with early IgA nephropathy (IgAN) and thin basement membrane nephropathy (TBMN), the most common causes presenting isolated hematuria in childhood, a proteomic approach of urinary exosomes from early IgAN and TBMN patients was introduced. The proteomic results from the patients were compared with a normal group to understand the pathophysiological processes associated with these diseases at the protein level. The urinary exosomes, which reflect pathophysiological processes, collected from three groups of young adults (early IgAN, TBMN, and normal) were trypsin-digested using a gel-assisted protocol, and quantified by label-free LC-MS/MS, using an MS(E) mode. A total of 1877 urinary exosome proteins, including cytoplasmic, membrane, and vesicle trafficking proteins, were identified. Among the differentially expressed proteins, four proteins (aminopeptidase N, vasorin precursor, α-1-antitrypsin, and ceruloplasmin) were selected as biomarker candidates to differentiate early IgAN from TBMN. We confirmed the protein levels of the four biomarker candidates by semi-quantitative immunoblot analysis in urinary exosomes independently prepared from other patients, including older adult groups. Further clinical studies are needed to investigate the diagnostic and prognostic value of these urinary markers for early IgAN and TBMN. Taken together, this study showed the possibility of identifying biomarker candidates for human urinary diseases using urinary exosomes and might help to understand the pathophysiology of early IgAN and TBMN at the protein level.
authors: Department of Molecular Medicine, School of Medicine, Kyungpook National University, Daegu, Republic of Korea.
authors: Pyong-Gon
authors: PG
authors: Moon
authors: Jeong-Eun
authors: JE
authors: Lee
authors: Sungyong
authors: S
authors: You
authors: Taek-Kyun
authors: TK
authors: Kim
authors: Ji-Hoon
authors: JH
authors: Cho
authors: In-San
authors: IS
authors: Kim
authors: Tae-Hwan
authors: TH
authors: Kwon
authors: Chan-Duck
authors: CD
authors: Kim
authors: Sun-Hee
authors: SH
authors: Park
authors: Daehee
authors: D
authors: Hwang
authors: Yong-Lim
authors: YL
authors: Kim
authors: Moon-Chang
authors: MC
authors: Baek