title: Dehydrocostus lactone prevents mitochondrial dysfunction in osteoblastic MC3T3-E1 cells.
abstract: The dried root of Saussurea lappa Clarke (Compositae) has been used as a traditional medicine. Dehydrocostus lactone is one of the main bioactive constituents of this medicinal plant. In the present study, the protective effect of dehydrocostus lactone against antimycin A (an inhibitor of mitochondrial complex III)-induced cytotoxicity was investigated in osteoblastic MC3T3-E1 cells. Pre-treatment with dehydrocostus lactone prior to antimycin A exposure significantly prevented mitochondrial membrane potential dissipation, complex IV inactivation, ATP loss, cytochrome c release, intracellular calcium elevation and potassium loss, and reactive oxygen species production induced by antimycin A. These results suggest that dehydrocostus lactone protects osteoblastic MC3T3-E1 cells from antimycin A-induced cell damage through the improved mitochondrial function.
authors: Department of Food & Nutrition, Education Graduate School, Kyung Hee University, Hoegi-dong, Dongdaemun-gu, Seoul, Republic of Korea. cheunmi@hanmail.net
authors: Eun Mi
authors: EM
authors: Choi