title: Proteome reference map of the skin mucus of Atlantic cod (Gadus morhua) revealing immune competent molecules.
abstract: The skin mucosal proteome of Atlantic cod (Gadus morhua) was mapped using a 2D PAGE, LC-MS/MS coupled approach. Mucosal proteins from naive fish were identified primarily by similarity searches across various cod EST databases. The identified proteins were clustered into 8 groups based on gene ontology classification for biological process. Most of the proteins identified from the gel are hitherto unreported for cod. Galectin-1, mannan binding lectin (MBL), serpins, cystatin B, cyclophilin A, FK-506 binding protein, proteasome subunits (alpha-3 and -7), ubiquitin, and g-type lysozyme are considered immune competent molecules. Five of the aforementioned proteins were cloned and their tissue distribution was analysed by RT-PCR.
authors: Aquatic Animal Health Research Group, Faculty of Biosciences and Aquaculture, University of Nordland, 8049 Bodø, Norway.
authors: Binoy
authors: B
authors: Rajan
authors: Jorge M O
authors: JM
authors: Fernandes
authors: Christopher M A
authors: CM
authors: Caipang
authors: Viswanath
authors: V
authors: Kiron
authors: Jan H W M
authors: JH
authors: Rombout
authors: Monica F
authors: MF
authors: Brinchmann