title: Assembly of a protein "brush" by end-grafting titin fragments to liposomes.
abstract: Titin is a giant filamentous protein of striated muscle composed of >300 immunoglobulin-like domains linked in tandem. Here, we demonstrate that a six-immunoglobulin fragment of titin carrying a poly-histidine tag forms a protein "brush" on liposomes containing metallochelating lipids. These specimens might allow the direct visualization by cryo-EM of frozen hydrated and unstained titin chains with preserved architectural features.
authors: Center for Cellular Imaging and Nanoanalytics (C-CINA), Biozentrum, University of Basel, Switzerland.
authors: Mohamed
authors: M
authors: Chami
authors: Barbara
authors: B
authors: Franke
authors: Olga
authors: O
authors: Mayans
authors: Laurent
authors: L
authors: Kreplak