title: Identification and molecular properties of SUMO-binding proteins in Arabidopsis.
abstract: Reversible conjugation of the small ubiquitin modifier (SUMO) peptide to proteins (SUMOylation) plays important roles in cellular processes in animals and yeasts. However, little is known about plant SUMO targets. To identify SUMO substrates in Arabidopsis and to probe for biological functions of SUMO proteins, we constructed 6xHis-3xFLAG fused AtSUMO1 (HFAtSUMO1) controlled by the CaMV35S promoter for transformation into Arabidopsis Col-0. After heat treatment, an increased sumoylation pattern was detected in the transgenic plants. SUMO1-modified proteins were selected after two-dimensional gel electrophoresis (2-DE) image analysis and identified using matrix-assisted laser-desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). We identified 27 proteins involved in a variety of processes such as nucleic acid metabolism, signaling, metabolism, and including proteins of unknown functions. Binding and sumoylation patterns were confirmed independently. Surprisingly, MCM3 (At5G46280), a DNA replication licensing factor, only interacted with and became sumoylated by AtSUMO1, but not by SUMO1ΔGG or AtSUMO3. The results suggest specific interactions between sumoylation targets and particular sumoylation enzymes.
authors: Division of Applied Life Science (Brain Korea 21 Program), and Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, Korea. hcpark@gnu.ac.kr
authors: Hyeong Cheol
authors: HC
authors: Park
authors: Wonkyun
authors: W
authors: Choi
authors: Hee Jin
authors: HJ
authors: Park
authors: Mi Sun
authors: MS
authors: Cheong
authors: Yoon Duck
authors: YD
authors: Koo
authors: Gilok
authors: G
authors: Shin
authors: Woo Sik
authors: WS
authors: Chung
authors: Woe-Yeon
authors: WY
authors: Kim
authors: Min Gab
authors: MG
authors: Kim
authors: Ray A
authors: RA
authors: Bressan
authors: Hans J
authors: HJ
authors: Bohnert
authors: Sang Yeol
authors: SY
authors: Lee
authors: Dae-Jin
authors: DJ
authors: Yun
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