title: pDNA/poly(L-lysine) Polyplexes Functionalized with a pH-Sensitive Charge-Conversional Poly(aspartamide) Derivative for Controlled Gene Delivery to Human Umbilical Vein Endothelial Cells.
abstract: An efficient endosome-escaping function was integrated into the polyplex of plasmid DNA (pDNA) with poly(L-lysine) (PLys) to improve its gene transfection efficiency through electrostatic coating with charge-conversional polymer (CCP). CCP showed charge-conversional function responding to endosomal pH, leading to the release of pDNA/PLys polyplex into the cytoplasm. The cells took up the intact CCP-integrated ternary polyplex, which exerted appreciably higher transfection efficiency with lower cytotoxicity than pDNA/PLys polyplex against human umbilical vein endothelial cells (HUVECs). This is consistent with the facilitated endosomal escape of the CCP-integrated ternary polyplex compared to the pDNA/PLys polyplex as directly observed with confocal laser-scanning microscopy.
authors: Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, Japan.
authors: Mai
authors: M
authors: Sanjoh
authors: Shigehiro
authors: S
authors: Hiki
authors: Yan
authors: Y
authors: Lee
authors: Makoto
authors: M
authors: Oba
authors: Kanjiro
authors: K
authors: Miyata
authors: Takehiko
authors: T
authors: Ishii
authors: Kazunori
authors: K
authors: Kataoka