title: Isolation in cell cultures and initial characterisation of two novel bocavirus species from swine in Northern Ireland.
abstract: We report the isolation in cell cultures of two novel bocavirus species in pigs from farms in Northern Ireland with clinical postweaning multisystemic wasting syndrome (PMWS). We have designated the isolates as porcine bocavirus-3 (PBoV3) and porcine bocavirus-4 (PBoV4). To date 5082 and 4125 bps of PBoV3 and PBoV4 have been sequenced, respectively. PBoV3 and PBoV4 show nucleotide homology to other known bocaviruses in swine and other organisms. Open reading frame (ORF) analysis has shown that these viruses have a third small ORF, equivalent to the NP1 ORF that distinguishes the bocaviruses from other parvoviruses. A panel of porcine field sera was screened by indirect immunofluorescence against both viruses. Of the 369 samples analysed, 32 (8.7%) and 35 (9.5%) sera were seropositive for PBoV3 and PBoV4 respectively, thus providing serological evidence of the exposure of swine in the field to bocavirus-like viruses. To date, the clinico-pathological significance of these novel swine bocaviruses, as primary pathogens or as immunosuppresive triggers for other infectious agents, is undetermined.
authors: Veterinary Sciences Division, Agri-Food and Biosciences Institute, Stoney Road, Stormont, Belfast BT4 3SD, United Kingdom. john.mckillen@afbini.gov.uk
authors: John
authors: J
authors: McKillen
authors: Francis
authors: F
authors: McNeilly
authors: Catherine
authors: C
authors: Duffy
authors: Michael
authors: M
authors: McMenamy
authors: Irene
authors: I
authors: McNair
authors: Bernt
authors: B
authors: Hjertner
authors: Andrena
authors: A
authors: Millar
authors: Karen
authors: K
authors: McKay
authors: Paula
authors: P
authors: Lagan
authors: Brian
authors: B
authors: Adair
authors: Gordon
authors: G
authors: Allan