title: Identification of novel mammalian phospholipids containing threonine, aspartate, and glutamate as the base moiety.
abstract: In this study, we showed the occurrence of phosphatidyl-L-threonine (PThr), phosphatidyl-L-aspartate (PAsp), and phosphatidyl-L-glutamate (PGlu) in rat brain. Analyses using an HPLC-ESI-MS and an amino acid analyzer showed the presence of L-threonine, L-aspartate, and L-glutamate in the acid-hydrolysates of phospholipids from porcine cerebrum, rat cerebrum, and rat liver. Results of ESI-MS/MS analyses with neutral loss scanning and product ion scanning suggest the presence of PThr-(18:0, 18:1), PThr-(18:0, 22:6), PAsp-(18:0, 18:1), PAsp-(18:0, 22:6), PGlu-(18:0, 18:1), and PGlu-(18:0, 22:6) in rat brain. This is the first study to identify 2 novel phospholipids, PAsp and PGlu, with a carboxylate-phosphate anhydride bond, in living organisms.
authors: Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011, Japan.
authors: Taketo
authors: T
authors: Omori
authors: Ai
authors: A
authors: Honda
authors: Hisaaki
authors: H
authors: Mihara
authors: Tatsuo
authors: T
authors: Kurihara
authors: Nobuyoshi
authors: N
authors: Esaki