title: Proteomic comparison of virulent phase I and avirulent phase II of Coxiella burnetii, the causative agent of Q fever.
abstract: Coxiella burnetii, a category B biological warfare agent, causes multiple outbreaks of the zoonotic disease Q fever world-wide, each year. The virulent phase I and avirulent phase II variants of the Nine Mile RSA 493 and 439 strains of C. burnetii were propagated in embryonated hen eggs and then purified by centrifugation through Renografin gradients. Total protein fractions were isolated from each phase and subjected to analysis by one-dimensional electrophoresis plus tandem mass spectrometry. A total of 235 and 215 non-redundant proteins were unambiguously identified from the phase I and II cells, respectively. Many of these proteins had not been previously reported in proteomic studies of C. burnetii. The newly identified proteins should provide additional insight into the pathogenesis of Q fever. Several of the identified proteins are involved in the biosynthesis and metabolism of components of the extracellular matrix. Forty-four of the proteins have been annotated as having distinct roles in the pathogenesis or survival of C. burnetii within the harsh phagolysosomal environment. We propose that nine enzymes specifically involved with lipopolysaccharide biosynthesis and metabolism, and that are distinctively present in phase I cells, are virulence-associated proteins.
authors: Institute of Virology, Slovak Academy of Sciences, Bratislava, Slovakia. viruludo@savba.sk
authors: Ludovit
authors: L
authors: Skultety
authors: Martin
authors: M
authors: Hajduch
authors: Gabriela
authors: G
authors: Flores-Ramirez
authors: Ján A
authors: JA
authors: Miernyk
authors: Fedor
authors: F
authors: Ciampor
authors: Rudolf
authors: R
authors: Toman
authors: Zuzana
authors: Z
authors: Sekeyova