title: Halomonas sp. OKOH--a marine bacterium isolated from the bottom sediment of Algoa Bay--produces a polysaccharide bioflocculant: partial characterization and biochemical analysis of its properties.
abstract: A bioflocculant-producing bacterium isolated from seawater was identified based on 16S rRNA gene nucleotide sequence to have 99% similarity to that of Halomonas sp. Au160H and the nucleotide sequence was deposited as Halomonas sp. OKOH (Genbank accession number is HQ875722). Influences of carbon source, nitrogen source, salt ions and pH on flocculating activity were investigated. The bioflocculant was optimally produced when glucose (87% flocculating activity) and urea (88% flocculating activity) were used as sources of carbon and nitrogen, respectively. Also, initial pH of 7.0 and Ca²⁺ supported optimal production of the bioflocculant with flocculating activities of 87% respectively. Chemical analyses revealed the bioflocculant to be a polysaccharide.
authors: Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry and Microbiology, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa.
authors: Leonard V
authors: LV
authors: Mabinya
authors: Sekelwa
authors: S
authors: Cosa
authors: Noxolo
authors: N
authors: Mkwetshana
authors: Anthony I
authors: AI
authors: Okoh
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