title: On-chip constructive cell-network study (I): contribution of cardiac fibroblasts to cardiomyocyte beating synchronization and community effect.
abstract: BACKGROUND
  To clarify the role of cardiac fibroblasts in beating synchronization, we have made simple lined-up cardiomyocyte-fibroblast network model in an on-chip single-cell-based cultivation system.
  
  
  RESULTS
  The synchronization phenomenon of two cardiomyocyte networks connected by fibroblasts showed (1) propagation velocity of electrophysiological signals decreased a magnitude depending on the increasing number of fibroblasts, not the lengths of fibroblasts; (2) fluctuation of interbeat intervals of the synchronized two cardiomyocyte network connected by fibroblasts did not always decreased, and was opposite from homogeneous cardiomyocyte networks; and (3) the synchronized cardiomyocytes connected by fibroblasts sometimes loses their synchronized condition and recovered to synchronized condition, in which the length of asynchronized period was shorter less than 30 beats and was independent to their cultivation time, whereas the length of synchronized period increased according to cultivation time.
  
  
  CONCLUSIONS
  The results indicated that fibroblasts can connect cardiomyocytes electrically but do not significantly enhance and contribute to beating interval stability and synchronization. This might also mean that an increase in the number of fibroblasts in heart tissue reduces the cardiomyocyte 'community effect', which enhances synchronization and stability of their beating rhythms.
authors: Department of Biomedical Information, Division of Biosystems, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Tokyo, Japan.
authors: Tomoyuki
authors: T
authors: Kaneko
authors: Fumimasa
authors: F
authors: Nomura
authors: Kenji
authors: K
authors: Yasuda
references: Pflugers Arch. 2003 Apr;446(1):125-32
references: 12690471
references: Lab Chip. 2003 Nov;3(4):292-6
references: 15007461
references: Circ Res. 2004 Apr 2;94(6):828-35
references: 14976125
references: Biochem Biophys Res Commun. 2006 Dec 8;351(1):209-15
references: 17055457
references: Exp Cell Res. 1970 Nov;63(1):124-30
references: 5531475
references: Analyst. 2007 Sep;132(9):892-8
references: 17710264
references: Proc Natl Acad Sci U S A. 2009 Jul 21;106(29):11954-9
references: 19515816
references: J Nanobiotechnology. 2004 Sep 9;2(1):9
references: 15357869
references: Biochem Biophys Res Commun. 2009 Aug 7;385(4):497-502
references: 19464263
references: Lab Chip. 2002 May;2(2):125-32
references: 15100846
references: Circ Res. 1991 Jan;68(1):114-30
references: 1984856
references: Exp Cell Res. 1969 Dec;58(2):420-6
references: 4998297
references: Biochem Biophys Res Commun. 2007 May 4;356(2):494-8
references: 17359936
references: Circ Res. 2003 Sep 5;93(5):381-3
references: 12958139
references: Exp Cell Res. 1974 Mar 15;84(1):223-34
references: 4817722
references: Circ Res. 2004 Apr 2;94(6):709-11
references: 15059940
references: Microsc Microanal. 2005 Jun;11(3):249-59
references: 16060978
references: Circ Res. 2003 Sep 5;93(5):421-8
references: 12893743
references: J Nanobiotechnology. 2004 Jul 1;2(1):7
references: 15230976
references: J Nanobiotechnology. 2005 May 31;3(1):4
references: 15927047
references: Circ Res. 2006 Mar 31;98(6):801-10
references: 16484613
references: J Physiol. 2007 Dec 1;585(Pt 2):565-78
references: 17932143