title: allB, allantoin utilisation and Salmonella enterica serovar Enteritidis and Typhimurium colonisation of poultry and mice.
abstract: Natural variation in the presence or the absence of STM0517-0529 genes allowing allantoin utilisation has been described in field isolates of the multidrug resistant Salmonella enterica serovar Typhimurium belonging to the phage type DT104. Interestingly, S. enterica subspecies enterica serovar Typhimurium DT104 is quite frequent in pigs and cattle, but rarely present in egg-laying hens. Taking into account the different mode of allantoin metabolism in birds and mammals, we were interested in whether the absence of STM0517-0529 genes may disable this clone in poultry colonisation. We have therefore constructed the allB (also designated as STM0523) mutants in S. enterica subspecies enterica serovar Typhimurium and S. enterica subspecies enterica serovar Enteritidis, and with these, we infected mice, newly hatched chickens and adult egg-laying hens to show that the defect in allantoin utilisation does not influence S. enterica virulence for mice or adult hens, but slightly decreases virulence of S. enterica for chickens. The decrease in virulence of the allB mutant was relatively minor as it could be observed only after a mixed infection model, consistent with a lower prevalence, but not a total absence of such clones in poultry flocks.
authors: Veterinary Research Institute, Hudcova 70, 621 00, Brno, Czech Republic.
authors: Jitka
authors: J
authors: Matiasovicova
authors: Hana
authors: H
authors: Havlickova
authors: Frantisek
authors: F
authors: Sisak
authors: Lenka
authors: L
authors: Pilousova
authors: Ivan
authors: I
authors: Rychlik
references: Appl Environ Microbiol. 2005 Mar;71(3):1616-25
references: 15746367
references: Arch Microbiol. 2007 May;187(5):415-24
references: 17180672
references: Electrophoresis. 2007 Feb;28(3):381-7
references: 17177243
references: Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6640-5
references: 10829079
references: Vet Microbiol. 2004 Jan 14;98(1):37-43
references: 14738780
references: Free Radic Res. 2000 Mar;32(3):235-44
references: 10730822
references: Vet Microbiol. 2009 Jan 1;133(1-2):193-8
references: 18723300
references: Insect Biochem Mol Biol. 1998 Dec;28(12):979-86
references: 9887513
references: J Insect Physiol. 2006 Aug;52(8):816-25
references: 16797581
references: Microbiology (Reading). 2008 May;154(Pt 5):1384-1389
references: 18451047
references: Vet Microbiol. 2008 Apr 30;128(3-4):414-8
references: 18054179
references: J Comp Physiol B. 2006 Sep;176(7):653-61
references: 16705445
references: Comp Biochem Physiol C Toxicol Pharmacol. 2010 Jan;151(1):12-7
references: 19654053
references: J Surg Res. 2007 Jan;137(1):96-102
references: 17064732
references: J Infect Dis. 2005 Jul 1;192(1):117-28
references: 15942901
references: Diagn Microbiol Infect Dis. 2008 Sep;62(1):1-6
references: 18486404
references: Infect Immun. 2004 Jul;72(7):3783-92
references: 15213119
references: J Bacteriol. 1999 Dec;181(24):7479-84
references: 10601204
references: Comp Biochem Physiol B Biochem Mol Biol. 2003 Jun;135(2):325-35
references: 12798942