title: Dark cell change of the cerebellar Purkinje cells induced by terbutaline under transient disruption of the blood-brain barrier in adult rats: morphological evaluation.
abstract: This study aimed to establish a cerebellar degeneration animal model and to characterize the dark cell change of Purkinje cells. We hypothesized that terbutaline, a β2-adrenoceptor agonist, induces cerebellar degeneration not only in neonatal rats, but also in adult rats. Nine-week-old adult male Sprague-Dawley rats were anesthetized and infused with 25% mannitol via the left common carotid artery. Thirty seconds later, terbutaline was infused via the same artery. Dark-stained Purkinje cells were observed in the entire cerebellum on day 3. Prominent Bergmann glial cells accompanied by swelling of the glial processes were present, and were closely associated with the dark-stained Purkinje cells. These findings were found continuously throughout day 30. Ultrastructurally, dilated Golgi vesicles and/or endoplasmic reticulum and large lamella bodies were present in both severely changed and slightly changed Purkinje cells. Bergmann glial cells in the area of synaptic contacts of the severely changed Purkinje cells showed swelling. The Bergmann glial process in close contact with the slightly changed Purkinje cell dendrite in molecular layer showed slight swelling, and large lamella bodies in the dendrite were observed close to the dendritic spines. These findings may suggest that terbutaline induced a failure of Bergmann glial cell and resulted in dark cell degeneration of the Purkinje cells due to glutamate excitotoxicity.
authors: Pathology Division, Mitsubishi Chemical Medience Corporation, 14 Sunayama, Kamisu-shi, Ibaraki, 314-0255, Japan. yamada.naoaki@mn.medience.co.jp
authors: Naoaki
authors: N
authors: Yamada
authors: Satoshi
authors: S
authors: Sasaki
authors: Hiroyuki
authors: H
authors: Ishii
authors: Junko
authors: J
authors: Sato
authors: Takeshi
authors: T
authors: Kanno
authors: Yumi
authors: Y
authors: Wako
authors: Minoru
authors: M
authors: Tsuchitani