title: Cross-species amplification and development of new microsatellite loci for Taxus wallichiana (Taxaceae).
abstract: OBJECTIVE
  Previously published and newly developed microsatellite primers for Taxus species were screened for their potential transfer to Taxus wallichiana and to characterize the genetic diversity and population structure of this species.
  
  
  RESULTS
  Twenty microsatellite markers that successfully PCR amplified showed polymorphisms when tested on T. wallichiana. Ten of these were obtained by cross-species amplification. The remaining 10 markers were developed in the current study. These markers were characterized on 58 individuals from two T. wallichiana populations from China. Overall, the number of alleles per locus ranged from two to twelve. No significant linkage disequilibrium (LD) was detected between the comparisons of these loci.
  
  
  CONCLUSIONS
  The polymorphic microsatellite markers screened in this study will be useful for further investigations of the conservation genetics of the endangered species T. wallichiana.
authors: Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming Yunnan 650204, China.
authors: Jie
authors: J
authors: Liu
authors: Lian-Ming
authors: LM
authors: Gao
authors: De-Zhu
authors: DZ
authors: Li
authors: De-Quan
authors: DQ
authors: Zhang
authors: Michael
authors: M
authors: Möller