title: Bordetella avium causes induction of apoptosis and nitric oxide synthase in turkey tracheal explant cultures.
abstract: Bordetellosis is an upper respiratory disease of turkeys caused by Bordetella avium in which the bacteria attach specifically to ciliated respiratory epithelial cells. Little is known about the mechanisms of pathogenesis of this disease, which has a negative impact in the commercial turkey industry. In this study, we produced a novel explant organ culture system that was able to successfully reproduce pathogenesis of B. avium in vitro, using tracheal tissue derived from 26 day-old turkey embryos. Treatment of the explants with whole cells of B. avium virulent strain 197N and culture supernatant, but not lipopolysaccharide (LPS) or tracheal cytotoxin (TCT), specifically induced apoptosis in ciliated cells, as shown by annexin V and TUNEL staining. LPS and TCT are known virulence factors of Bordetella pertussis, the causative agent of whooping cough. Treatment with whole cells of B. avium and LPS specifically induced NO response in ciliated cells, shown by uNOS staining and diaphorase activity. The explant system is being used as a model to elucidate specific molecules responsible for the symptoms of bordetellosis.
authors: Department of Biology, Drew University, Madison, NJ 07940, USA.
authors: David M
authors: DM
authors: Miyamoto
authors: Kristin
authors: K
authors: Ruff
authors: Nathan M
authors: NM
authors: Beach
authors: Stephanie B
authors: SB
authors: Stockwell
authors: Angella
authors: A
authors: Dorsey-Oresto
authors: Isaac
authors: I
authors: Masters
authors: Louise M
authors: LM
authors: Temple
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