title: Covalent stabilization of a small molecule-RNA complex.
abstract: We demonstrate covalent bond formation between an RNA aptamer containing a cysteamine-tethered nucleobase and helix-threading peptides (HTPs) containing α-bromoacetamide N-termini. The reaction is high yielding and inhibited by a DNA strand Watson-Crick complementary to the aptamer sequence indicating covalent reaction is dependent on the high affinity HTP-binding site present in the folded aptamer. These results are important for future structural studies of HTP-RNA complexes and methods for the discovery of new high affinity analogs via covalent tethering strategies.
authors: Department of Chemistry, University of California, One Shields Ave, Davis, CA 95616, United States.
authors: Hayden
authors: H
authors: Peacock
authors: Radhika
authors: R
authors: Bachu
authors: Peter A
authors: PA
authors: Beal
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