title: [Cisplatin induces drug resistance in human esophageal squamous carcinoma cell line EC109 by decreasing CTR1 protein expression].
abstract: OBJECTIVE
  To investigate the mechanism of the development of cisplatin resistance in a human esophageal squamous carcinoma cell line.
  
  
  METHODS
  The cytotoxicity of cisplatin in the cisplatin-resistant resistant cell line EC109/CDDP and its parental cell line EC109 was measured by MTT assay. Whole-cell cisplatin accumulation and Pt-DNA adduct formation were determined by inductively coupled plasma mass spectrometry (ICP-MS). Western blotting was used to investigate the protein expression of full length PARP, cleaved PARP, and copper transporter 1 (CTR1).
  
  
  RESULTS
  EC109/CDDP cells was more resistant to cisplatin-induced cytotoxicity and apoptosis than EC109 cells. Compared with EC109 cells, EC109/CDDP cells exhibited less cisplatin accumulation and Pt-DNA adduct formation with also decreased CTR1 protein expression.
  
  
  CONCLUSIONS
  Cisplatin induces drug resistant phenotype by decreasing the protein level of CTR1, which controls cell accumulation and cytotoxic effect of cisplatin.
authors: School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China. yule0423@gmail.com
authors: Le
authors: L
authors: Yu
authors: Ming-hui
authors: MH
authors: Chen
authors: Chun-ping
authors: CP
authors: Gu
authors: Yi-lei
authors: YL
authors: Li
authors: Jing
authors: J
authors: Wen
authors: Jian-hua
authors: JH
authors: Fu
authors: Chi-hin
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authors: Cho
authors: Shu-wen
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authors: Liu