title: An adsorptive transfer technique coupled with brdicka reaction to reveal the importance of metallothionein in chemotherapy with platinum based cytostatics.
abstract: The drugs based on platinum metals represent one of the oldest, but also one of the most effective groups of chemotherapeutic agents. Thanks to many clinical studies it is known that resistance of tumor cells to drugs is a frequent cause of chemotherapy failure. With regard to platinum based drugs, multidrug resistance can also be connected with increased expression of low-molecular weight protein metallothionein (MT). This study aimed at investigating the interactions of MT with cisplatin or carboplatin, using the adsorptive transfer technique coupled with differential pulse voltammetry Brdicka reaction (AdTS DPV Brdicka reaction), and a comparison of in vitro results with results obtained in vivo. The results obtained from the in vitro study show a strong affinity between platinum based drugs and MT. Further, we analyzed extracts of neuroblastoma cell lines treated with cisplatin or carboplatin. It is clear that neuroblastoma UKF-NB-4 cisplatin-resistant and cisplatin-sensitive cell lines unlikely respond to the presence of the platinum-based cytostatics cisplatin and carboplatin. Finally, we determined the level of MT in samples from rabbits treated with carboplatin and patients with retinoblastoma treated with the same drug.
authors: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic.
authors: Sona
authors: S
authors: Krizkova
authors: Ivo
authors: I
authors: Fabrik
authors: Dalibor
authors: D
authors: Huska
authors: Vojtech
authors: V
authors: Adam
authors: Petr
authors: P
authors: Babula
authors: Jan
authors: J
authors: Hrabeta
authors: Tomas
authors: T
authors: Eckschlager
authors: Pavel
authors: P
authors: Pochop
authors: Denisa
authors: D
authors: Darsova
authors: Jiri
authors: J
authors: Kukacka
authors: Richard
authors: R
authors: Prusa
authors: Libuse
authors: L
authors: Trnkova
authors: Rene
authors: R
authors: Kizek
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