title: Genotypic prediction of human immunodeficiency virus type 1 tropism by use of plasma and peripheral blood mononuclear cells in the routine clinical laboratory.
abstract: We developed a sequencing assay for genotypic HIV-1 tropism determination. The assay allows examination of HIV RNA from plasma and HIV DNA from peripheral blood mononuclear cells (PBMC), including PBMC samples from patients with undetectable viral loads. Assessment of 100 pairs of plasma and PBMC samples showed a high concordance of 90%. With the limitations of population-based sequencing, the assay was found to be robust and suitable for the routine clinical laboratory.
authors: Institute of Laboratory Medicine1 and Department of Dermatology,2 General Hospital Linz, Krankenhausstrasse 9, A-4020 Linz, Austria.
authors: Christian
authors: C
authors: Paar
authors: Maria
authors: M
authors: Geit
authors: Herbert
authors: H
authors: Stekel
authors: Jörg
authors: J
authors: Berg
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