title: From SELEX to cell dual selections for synthetic riboswitches.
abstract: Synthetic riboswitches have emerged as useful tools for controlling gene expression to reprogram cellular behavior. However, advancing beyond proof-of-principle experiments requires the ability to quickly generate new synthetic riboswitches from RNA libraries. In this chapter, we provide a step-by-step overview of the process of obtaining synthetic riboswitches for use in Escherichia coli, starting from a randomized RNA library.
authors: Department of Chemistry and Center for Fundamental and Applied Molecular Evolution, Emory University, Atlanta, Georgia, USA.
authors: Joy
authors: J
authors: Sinha
authors: Shana
authors: S
authors: Topp
authors: Justin P
authors: JP
authors: Gallivan