title: A genome-wide genetic map of NB-LRR disease resistance loci in potato.
abstract: Like all plants, potato has evolved a surveillance system consisting of a large array of genes encoding for immune receptors that confer resistance to pathogens and pests. The majority of these so-called resistance or R proteins belong to the super-family that harbour a nucleotide binding and a leucine-rich-repeat domain (NB-LRR). Here, sequence information of the conserved NB domain was used to investigate the genome-wide genetic distribution of the NB-LRR resistance gene loci in potato. We analysed the sequences of 288 unique BAC clones selected using filter hybridisation screening of a BAC library of the diploid potato clone RH89-039-16 (S. tuberosum ssp. tuberosum) and a physical map of this BAC library. This resulted in the identification of 738 partial and full-length NB-LRR sequences. Based on homology of these sequences with known resistance genes, 280 and 448 sequences were classified as TIR-NB-LRR (TNL) and CC-NB-LRR (CNL) sequences, respectively. Genetic mapping revealed the presence of 15 TNL and 32 CNL loci. Thirty-six are novel, while three TNL loci and eight CNL loci are syntenic with previously identified functional resistance genes. The genetic map was complemented with 68 universal CAPS markers and 82 disease resistance trait loci described in literature, providing an excellent template for genetic studies and applied research in potato.
authors: Laboratory of Nematology, Wageningen University and Research Centre, Droevendaalsesteeg 1, Wageningen, The Netherlands. erin.bakker@wur.nl
authors: Erin
authors: E
authors: Bakker
authors: Theo
authors: T
authors: Borm
authors: Pjotr
authors: P
authors: Prins
authors: Edwin
authors: E
authors: van der Vossen
authors: Gerda
authors: G
authors: Uenk
authors: Marjon
authors: M
authors: Arens
authors: Jan
authors: J
authors: de Boer
authors: Herman
authors: H
authors: van Eck
authors: Mariëlle
authors: M
authors: Muskens
authors: Jack
authors: J
authors: Vossen
authors: Gerard
authors: G
authors: van der Linden
authors: Roeland
authors: R
authors: van Ham
authors: Rene
authors: R
authors: Klein-Lankhorst
authors: Richard
authors: R
authors: Visser
authors: Geert
authors: G
authors: Smant
authors: Jaap
authors: J
authors: Bakker
authors: Aska
authors: A
authors: Goverse
references: Theor Appl Genet. 2005 Sep;111(5):879-87
references: 16044270
references: Genetics. 2006 Jun;173(2):1075-87
references: 16582432
references: Cell. 1994 Sep 23;78(6):1101-15
references: 7923359
references: Proc Natl Acad Sci U S A. 2002 Feb 19;99(4):2404-9
references: 11842188
references: Plant J. 2002 Feb;29(4):439-51
references: 11846877
references: Plant J. 2002 Jul;31(2):127-36
references: 12121443
references: Nucleic Acids Res. 2005 Jan 1;33(Database issue):D666-70
references: 15608284
references: BMC Genomics. 2007 May 02;8:112
references: 17474978
references: Genetics. 2000 May;155(1):407-20
references: 10790413
references: Plant J. 2000 Sep;23(5):567-76
references: 10972883
references: Theor Appl Genet. 2002 Mar;104(4):669-674
references: 12582672
references: Plant Cell. 1997 Apr;9(4):521-32
references: 9144960
references: Proc Natl Acad Sci U S A. 2003 Dec 9;100(25):15253-8
references: 14645721
references: Theor Appl Genet. 2004 Jul;109(2):384-93
references: 15057419
references: Plant Cell. 1998 Nov;10(11):1861-74
references: 9811794
references: Plant J. 2003 Dec;36(6):867-82
references: 14675451
references: Plant Cell. 1998 Nov;10(11):1833-46
references: 9811792
references: Theor Appl Genet. 2006 May;112(7):1232-8
references: 16453130
references: Plant Mol Biol. 2006 Nov;62(4-5):669-82
references: 16941217
references: Theor Appl Genet. 2008 Jan;116(2):297-303
references: 17985110
references: Genetics. 2009 Aug;182(4):1351-64
references: 19474202
references: Plant Cell. 1997 Apr;9(4):641-51
references: 9144966
references: Theor Appl Genet. 2007 Apr;114(6):1051-8
references: 17394033
references: Plant Cell. 2002 Dec;14(12):3149-62
references: 12468733
references: Genetics. 1995 Jan;139(1):463-71
references: 7705647
references: Mol Gen Genet. 1998 Feb;257(3):376-85
references: 9520273
references: Plant J. 2005 Oct;44(1):37-51
references: 16167894
references: Plant Mol Biol. 2003 Jul;52(5):1037-49
references: 14558663
references: Plant J. 2000 Jan;21(1):73-81
references: 10652152
references: Genome Res. 2002 Dec;12(12):1871-84
references: 12466291
references: Theor Appl Genet. 2010 Feb;120(3):679-89
references: 19882336
references: Genetics. 2003 Jun;164(2):655-64
references: 12807786
references: Plant J. 2002 May;30(3):361-71
references: 12000683
references: Plant Cell. 2001 Feb;13(2):337-50
references: 11226189
references: Mol Plant Microbe Interact. 1995 Nov-Dec;8(6):886-91
references: 8664498
references: Cell. 1996 Jul 12;86(1):123-33
references: 8689679
references: Theor Appl Genet. 2009 Feb;118(3):565-80
references: 19005638
references: Plant Cell. 2003 Apr;15(4):809-34
references: 12671079
references: Plant Mol Biol. 2008 Apr;66(6):619-36
references: 18247136
references: Plant Cell. 1999 May;11(5):781-92
references: 10330465
references: Plant Physiol. 2005 Jul;138(3):1310-7
references: 16010005
references: Proc Natl Acad Sci U S A. 1998 Feb 17;95(4):1663-8
references: 9465073
references: Theor Appl Genet. 2005 Aug;111(3):591-7
references: 15909136
references: Plant J. 2000 Jan;21(2):177-88
references: 10743658
references: Bioinformatics. 2010 Oct 15;26(20):2617-9
references: 20739307
references: Mol Plant Microbe Interact. 2009 Jun;22(6):630-41
references: 19445588
references: Plant J. 2004 Apr;38(2):285-97
references: 15078331
references: Mol Plant Microbe Interact. 2008 Jun;21(6):757-68
references: 18624640
references: Plant Cell. 2000 May;12(5):663-76
references: 10810142
references: Plant Cell. 1999 Mar;11(3):495-506
references: 10072407
references: Plant Mol Biol. 2007 Sep;65(1-2):93-106
references: 17611798
references: Genome Res. 1998 Nov;8(11):1113-30
references: 9847076
references: Mol Plant Microbe Interact. 2001 Dec;14(12):1420-5
references: 11768537
references: Genetics. 2007 Aug;176(4):2541-9
references: 17507669
references: Cell. 1994 Sep 23;78(6):1089-99
references: 7923358
references: Genetics. 2000 Jun;155(2):873-87
references: 10835406
references: Proc Natl Acad Sci U S A. 1991 Mar 15;88(6):2336-40
references: 2006172
references: Genetics. 2003 Dec;165(4):2107-16
references: 14704190
references: Mol Gen Genet. 1992 Jan;231(2):179-85
references: 1346546
references: Plant J. 2005 Oct;44(2):208-22
references: 16212601
references: Theor Appl Genet. 2003 May;106(8):1517-23
references: 12750795
references: Plant J. 2004 Feb;37(4):528-38
references: 14756761
references: Nat Biotechnol. 1998 Dec;16(13):1365-9
references: 9853621
references: Plant Cell. 2004 Feb;16(2):309-18
references: 14742871
references: Theor Appl Genet. 1995 Mar;90(3-4):451-6
references: 24173937
references: Theor Appl Genet. 2002 Sep;105(4):564-567
references: 12582505
references: Science. 1995 Aug 11;269(5225):843-6
references: 7638602
references: Plant J. 2005 Apr;42(2):251-61
references: 15807786
references: Nat Genet. 1996 Dec;14(4):421-9
references: 8944022
references: Plant Cell. 2000 Nov;12(11):2033-46
references: 11090207
references: Plant J. 2007 Apr;50(1):14-28
references: 17346268
references: Mol Gen Genet. 1991 May;227(1):81-5
references: 1675423
references: Curr Biol. 2005 May 24;15(10):968-73
references: 15916955
references: Plant Cell. 1998 Nov;10(11):1847-60
references: 9811793
references: Plant Cell. 1994 Apr;6(4):511-20
references: 7911348
references: Plant J. 1999 Jul;19(1):55-64
references: 10417726
references: Theor Appl Genet. 2003 May;106(8):1524-31
references: 12750796
references: Plant Cell. 2001 Jan;13(1):163-78
references: 11158537
references: Science. 1994 Sep 23;265(5180):1856-60
references: 8091210
references: Plant Cell. 1997 Jun;9(6):879-94
references: 9212464
references: Theor Appl Genet. 2006 Feb;112(4):699-707
references: 16365760
references: Plant J. 1999 Nov;20(3):265-77
references: 10571887
references: Theor Appl Genet. 2004 Nov;109(8):1604-9
references: 15448896
references: Plant Cell. 1998 Sep;10(9):1439-52
references: 9724691
references: Plant J. 2004 Jun;38(6):898-909
references: 15165183
references: Theor Appl Genet. 2011 Feb;122(3):595-608
references: 21049265
references: Plant Cell Rep. 2007 Jul;26(7):895-905
references: 17310335
references: Mol Genet Genomics. 2008 Sep;280(3):187-98
references: 18563445
references: Mol Gen Genet. 1990 Nov;224(2):177-82
references: 1980523
references: Plant J. 2004 Jan;37(1):46-60
references: 14675431
references: Proc Natl Acad Sci U S A. 1999 Nov 23;96(24):14153-8
references: 10570214
references: Genetics. 2007 Nov;177(3):1871-80
references: 17947408
references: Mol Genet Genomics. 2004 May;271(4):402-15
references: 15014983
references: Mol Plant Microbe Interact. 2005 Nov;18(11):1186-94
references: 16353553
references: Genetics. 1988 Dec;120(4):1095-103
references: 17246486
references: Plant Cell. 1995 Aug;7(8):1195-206
references: 7549479
references: Mol Gen Genet. 1992 May;233(1-2):278-83
references: 1351246
references: Plant Cell. 2002 Jul;14(7):1457-67
references: 12119367
references: Annu Rev Phytopathol. 2001;39:79-102
references: 11701860
references: J Mol Biol. 1990 Oct 5;215(3):403-10
references: 2231712
references: Mol Plant Microbe Interact. 2002 Jul;15(7):717-27
references: 12118888
references: Plant Cell. 1999 Jul;11(7):1365-76
references: 10402435
references: Mol Plant Microbe Interact. 2004 Apr;17(4):428-35
references: 15077675
references: Nat Genet. 1993 Mar;3(3):266-72
references: 8485583
references: Plant Cell. 1998 Aug;10(8):1307-19
references: 9707531
references: Plant J. 2001 Sep;27(5):439-53
references: 11576428
references: Mol Genet Genomics. 2001 Dec;266(4):639-45
references: 11810236
references: Plant J. 1993 Aug;4(2):403-10
references: 8106085