title: Nanogyroids incorporating multivalent lipids: enhanced membrane charge density and pore forming ability for gene silencing.
abstract: The self-assembly of a custom-synthesized pentavalent cationic lipid (MVL5) and glycerol monooleate (GMO) with small interfering RNA (siRNA) results in the formation of a double-gyroid bicontinuous inverted cubic phase with colocalized lipid/siRNA domains as shown by synchrotron X-ray scattering and fluorescence microscopy. The high charge density (due to MVL5) and positive Gaussian modulus of the GMO-containing membranes confer optimal electrostatic and elastic properties for endosomal escape, enabling efficient siRNA delivery and effective, specific gene silencing.
authors: Department of Physics, University of California, Santa Barbara, California 93106, USA. cecilial@mrl.ucsb.edu
authors: Cecília
authors: C
authors: Leal
authors: Kai K
authors: KK
authors: Ewert
authors: Rahau S
authors: RS
authors: Shirazi
authors: Nathan F
authors: NF
authors: Bouxsein
authors: Cyrus R
authors: CR
authors: Safinya
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