title: The effect of surface treatments on the adhesion of electrochemically deposited hydroxyapatite coating to titanium and on its interaction with cells and bacteria.
abstract: The effect of different mechanical and chemical pre-treatments on the adhesion strength of hydroxyapatite (HAp) coating on a commercially pure titanium (CP-Ti) substrate was studied by means of a standard tensile test followed by microscopic and chemical analysis to determine the locus of fracture. In addition, the effects of either these pre-treatments or post-treatment by low-energy electron irradiation, which allowed tuning the wettability of the surface, on both osteoblast progenitor attachment and S. aureus bacteria attachment were investigated. A dedicated program was developed for unambiguous identification and count of stained cells. A single-phase HAp coating was formed by electrodeposition. A series of surface pre-treatments consisted of grinding down to P1000, etching in HNO₃/HF solution, grit blast, soaking in NaOH and subsequent heat treatment provided the highest adhesion strength to the HAp coating. Osteoblast progenitors derived from rats may be attached preferentially to a hydrophilic surface (post-treatment to θ = 30°), while the bacteria seemed to be less attached to hydrophobic surfaces (post-treatment to θ = 105°). However, the results were not statistically different. The bacteria seemed to be less attached to the smoother, uncoated surfaces.
authors: Materials Science and Engineering Program, Tel Aviv University, Ramat Aviv 69978, Israel. neliaz@eng.tau.ac.il
authors: Noam
authors: N
authors: Eliaz
authors: Oshrit
authors: O
authors: Ritman-Hertz
authors: Daniel
authors: D
authors: Aronov
authors: Evgeny
authors: E
authors: Weinberg
authors: Yotam
authors: Y
authors: Shenhar
authors: Gil
authors: G
authors: Rosenman
authors: Miron
authors: M
authors: Weinreb
authors: Eliora
authors: E
authors: Ron
references: J Biomed Mater Res A. 2003 Oct 1;67(1):26-35
references: 14517858
references: Acta Orthop Scand. 2003 Apr;74(2):140-5
references: 12807319
references: Acta Biomater. 2009 Oct;5(8):3178-91
references: 19409870
references: Nat Mater. 2003 Jul;2(7):457-60
references: 12819775
references: J Biomed Mater Res A. 2004 Nov 1;71(2):343-52
references: 15376266
references: N Engl J Med. 2004 Apr 1;350(14):1422-9
references: 15070792
references: J Biomed Mater Res A. 2005 Jul 1;74(1):49-58
references: 15924300
references: Biomaterials. 2005 Jun;26(16):3029-37
references: 15603798
references: J Biomed Mater Res. 1984 Apr;18(4):337-55
references: 6736072
references: J Colloid Interface Sci. 1997 Dec 15;196(2):136-147
references: 9792739
references: Biomaterials. 2006 Aug;27(23):4192-203
references: 16618502
references: J Biomed Mater Res. 2002 Apr;60(1):1-7
references: 11835153
references: Anal Chem. 2000 Jul 1;72(13):2886-94
references: 10905323
references: Biomaterials. 2004 Aug;25(19):4759-66
references: 15120522
references: J Biomed Mater Res. 1979 Jul;13(4):631-43
references: 110811
references: J Biomed Mater Res A. 2009 Apr;89(1):270-80
references: 18563813
references: Acta Biomater. 2009 Jul;5(6):2258-69
references: 19251497
references: J Biomed Mater Res A. 2007 Mar 1;80(3):621-34
references: 17051536
references: J Biomed Mater Res. 1994 Dec;28(12):1419-25
references: 7876280
references: J Mater Sci Mater Med. 2004 Sep;15(9):1021-9
references: 15448410
references: J Bone Joint Surg Am. 1995 Sep;77(9):1380-95
references: 7673290
references: J Orthop Res. 2000 May;18(3):485-93
references: 10937638
references: J Biomed Mater Res A. 2004 Mar 1;68(3):504-12
references: 14762930
references: FEMS Microbiol Lett. 1999 Apr 15;173(2):279-84
references: 10227156