title: Mitochondrial function and energy metabolism in umbilical cord blood- and bone marrow-derived mesenchymal stem cells.
abstract: Human mesenchymal stem cells (hMSCs) are an attractive choice for a variety of cellular therapies. hMSCs can be isolated from many different tissues and possess unique mitochondrial properties that can be used to determine their differentiation potential. Mitochondrial properties may possibly be used as a quality measure of hMSC-based products. Accordingly, the present work focuses on the mitochondrial function of hMSCs from umbilical cord blood (UCBMSC) cells and bone marrow cells from donors younger than 18 years of age (BMMSC <18) and those more than 50 years of age (BMMSC >50). Changes of ultrastructure and energy metabolism during osteogenic differentiation in all hMSC types were studied in detail. Results show that despite similar surface antigen characteristics, the UCBMSCs had smaller cell surface area and possessed more abundant rough endoplasmic reticulum than BMMSC >50. BMMSC <18 were morphologically more UCBMSC-like. UCBMSC showed dramatically higher mitochondrial-to-cytoplasm area ratio and elevated superoxide and manganese superoxide dismutase (MnSOD) levels as compared with BMMSC >50 and BMMSC <18. All hMSCs types showed changes indicative of mitochondrial activation after 2 weeks of osteogenic differentiation, and the increase in mitochondrial-to-cytoplasm area ratio appears to be one of the first steps in the differentiation process. However, BMMSC >50 showed a lower level of mitochondrial maturation and differentiation capacity. UCBMSCs and BMMSCs also showed a different pattern of exocytosed proteins and glycoproteoglycansins. These results indicate that hMSCs with similar cell surface antigen expression have different mitochondrial and functional properties, suggesting different maturation levels and other significant biological variations of the hMSCs. Therefore, it appears that mitochondrial analysis presents useful characterization criteria for hMSCs intended for clinical use.
authors: Department of Anatomy and Cell Biology, Institute of Biomedicine, University of Oulu, Oulu, Finland. mika.pietila@aaito.fi
authors: Mika
authors: M
authors: Pietilä
authors: Sami
authors: S
authors: Palomäki
authors: Siri
authors: S
authors: Lehtonen
authors: Ilja
authors: I
authors: Ritamo
authors: Leena
authors: L
authors: Valmu
authors: Johanna
authors: J
authors: Nystedt
authors: Saara
authors: S
authors: Laitinen
authors: Hannnu-Ville
authors: HV
authors: Leskelä
authors: Raija
authors: R
authors: Sormunen
authors: Juha
authors: J
authors: Pesälä
authors: Katrina
authors: K
authors: Nordström
authors: Ari
authors: A
authors: Vepsäläinen
authors: Petri
authors: P
authors: Lehenkari
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