title: Circadian regulation of intracellular G-protein signalling mediates intercellular synchrony and rhythmicity in the suprachiasmatic nucleus.
abstract: Synchronous oscillations of thousands of cellular clocks in the suprachiasmatic nucleus (SCN), the circadian centre, are coordinated by precisely timed cell-cell communication, the principle of which is largely unknown. Here we show that the amount of RGS16 (regulator of G protein signalling 16), a protein known to inactivate Gαi, increases at a selective circadian time to allow time-dependent activation of intracellular cyclic AMP signalling in the SCN. Gene ablation of Rgs16 leads to the loss of circadian production of cAMP and as a result lengthens circadian period of behavioural rhythm. The temporally precise regulation of the cAMP signal by clock-controlled RGS16 is needed for the dorsomedial SCN to maintain a normal phase-relationship to the ventrolateral SCN. Thus, RGS16-dependent temporal regulation of intracellular G protein signalling coordinates the intercellular synchrony of SCN pacemaker neurons and thereby defines the 24 h rhythm in behaviour.
authors: Department of Systems Biology, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto 606-8501, Japan.
authors: Masao
authors: M
authors: Doi
authors: Atsushi
authors: A
authors: Ishida
authors: Akiko
authors: A
authors: Miyake
authors: Miho
authors: M
authors: Sato
authors: Rie
authors: R
authors: Komatsu
authors: Fumiyoshi
authors: F
authors: Yamazaki
authors: Ikuo
authors: I
authors: Kimura
authors: Soken
authors: S
authors: Tsuchiya
authors: Hiroshi
authors: H
authors: Kori
authors: Kazuyuki
authors: K
authors: Seo
authors: Yoshiaki
authors: Y
authors: Yamaguchi
authors: Masahiro
authors: M
authors: Matsuo
authors: Jean-Michel
authors: JM
authors: Fustin
authors: Rina
authors: R
authors: Tanaka
authors: Yasuko
authors: Y
authors: Santo
authors: Hiroyuki
authors: H
authors: Yamada
authors: Yukari
authors: Y
authors: Takahashi
authors: Michihiro
authors: M
authors: Araki
authors: Kazuki
authors: K
authors: Nakao
authors: Shinichi
authors: S
authors: Aizawa
authors: Masaki
authors: M
authors: Kobayashi
authors: Karl
authors: K
authors: Obrietan
authors: Gozoh
authors: G
authors: Tsujimoto
authors: Hitoshi
authors: H
authors: Okamura
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