title: Manufacturing of adenovirus vectors: production and purification of helper dependent adenovirus.
abstract: Adenoviral vector (AdV) of the third generation also known as helper-dependent adenoviral vector (HDV) is an attractive delivery system for gene therapy applications. However, obtaining high quality-grade HDV in sufficient amount remains a challenge that hampers the extensive use of this vector in preclinical and clinical studies. Here we review recent progress in the large-scale manufacturing of HDV. The production of HDV is now amenable to large-scale volume with reduced process duration under optimized rescue and co-infection conditions. Also, efficient downstream processing of HDV with acceptable recovery of HDV and minimal contamination by the helper virus is described.
authors: Biotechnology Research Institute, NRC, Montreal, QC, Canada.
authors: Edwige
authors: E
authors: Dormond
authors: Amine A
authors: AA
authors: Kamen