title: miR-132 mediates the integration of newborn neurons into the adult dentate gyrus.
abstract: Neuronal activity enhances the elaboration of newborn neurons as they integrate into the synaptic circuitry of the adult brain. The role microRNAs play in the transduction of neuronal activity into growth and synapse formation is largely unknown. MicroRNAs can influence the expression of hundreds of genes and thus could regulate gene assemblies during processes like activity-dependent integration. Here, we developed viral-based methods for the in vivo detection and manipulation of the activity-dependent microRNA, miR-132, in the mouse hippocampus. We find, using lentiviral and retroviral reporters of miR-132 activity, that miR-132 is expressed at the right place and right time to influence the integration of newborn neurons. Retroviral knockdown of miR-132 using a specific 'sponge' containing multiple target sequences impaired the integration of newborn neurons into the excitatory synaptic circuitry of the adult brain. To assess potential miR-132 targets, we used a whole-genome microarray in PC12 cells, which have been used as a model of neuronal differentiation. miR-132 knockdown in PC12 cells resulted in the increased expression of hundreds of genes. Functional grouping indicated that genes involved in inflammatory/immune signaling were the most enriched class of genes induced by miR-132 knockdown. The correlation of miR-132 knockdown to increased proinflammatory molecular expression may indicate a mechanistic link whereby miR-132 functions as an endogenous mediator of activity-dependent integration in vivo.
authors: The Vollum Institute, Oregon Health and Science University, Portland, Oregon, United States of America. Bryan.W.Luikart@dartmouth.edu
authors: Bryan W
authors: BW
authors: Luikart
authors: AeSoon L
authors: AL
authors: Bensen
authors: Eric K
authors: EK
authors: Washburn
authors: Julia V
authors: JV
authors: Perederiy
authors: Kimmy G
authors: KG
authors: Su
authors: Yun
authors: Y
authors: Li
authors: Steven G
authors: SG
authors: Kernie
authors: Luis F
authors: LF
authors: Parada
authors: Gary L
authors: GL
authors: Westbrook
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