title: Engineering of a bis-chelator motif into a protein α-helix for rigid lanthanide binding and paramagnetic NMR spectroscopy.
abstract: Attachment of two nitrilotriacetic acid-based ligands to a protein α-helix in an i, i + 4 configuration produces an octadentate chelating motif that is able to bind paramagnetic lanthanide ions rigidly and with high affinity, leading to large pseudocontact shifts and residual dipolar couplings in the NMR spectrum.
authors: Medicinal Chemistry and Drug Action, Monash Institute of Pharmaceutical Sciences, Parkville, VIC 3052. james.swarbrick@monash.edu
authors: James D
authors: JD
authors: Swarbrick
authors: Phuc
authors: P
authors: Ung
authors: Xun-Cheng
authors: XC
authors: Su
authors: Ansis
authors: A
authors: Maleckis
authors: Sandeep
authors: S
authors: Chhabra
authors: Thomas
authors: T
authors: Huber
authors: Gottfried
authors: G
authors: Otting
authors: Bim
authors: B
authors: Graham