title: Interactions of the DNA polymerase X from African Swine Fever Virus with the ssDNA. Properties of the total DNA-binding site and the strong DNA-binding subsite.
abstract: Interactions of the polymerase X from the African Swine Fever Virus with the ssDNA have been studied, using quantitative fluorescence titration and fluorescence resonance energy transfer techniques. The primary DNA-binding subsite of the enzyme, independent of the DNA conformation, is located on the C-terminal domain. Association of the bound DNA with the catalytic N-terminal domain finalizes the engagement of the total DNA-binding site of the enzyme and induces a large topological change in the structure of the bound ssDNA. The free energy of binding includes a conformational transition of the protein. Large positive enthalpy changes accompanying the ASFV pol X-ssDNA association indicate that conformational changes of the complex are induced by the engagement of the N-terminal domain. The enthalpy changes are offset by large entropy changes accompanying the DNA binding to the C-terminal domain and the total DNA-binding site, predominantly resulting from the release of water molecules.
authors: Department of Biochemistry and Molecular Biology, The University of Texas Medical Branch at Galveston, 77555–1053, United States.
authors: Maria J
authors: MJ
authors: Jezewska
authors: Michal R
authors: MR
authors: Szymanski
authors: Wlodzimierz
authors: W
authors: Bujalowski
references: J Mol Biol. 2007 Oct 12;373(1):75-95
references: 17765921
references: Nat Struct Biol. 2001 Nov;8(11):936-41
references: 11685238
references: Biophys Chem. 1978 Sep;8(4):327-39
references: 728537
references: J Mol Biol. 1976 Oct 25;107(2):145-58
references: 1003464
references: Biochemistry. 1998 Mar 3;37(9):3116-36
references: 9485465
references: Biochemistry. 1996 Feb 20;35(7):2117-28
references: 8652554
references: Biochemistry. 1998 Oct 6;37(40):14300-16
references: 9760268
references: J Mol Biol. 1998 Dec 11;284(4):1113-31
references: 9837730
references: J Mol Biol. 1974 Jun 25;86(2):469-89
references: 4416620
references: Biochemistry. 2000 Oct 24;39(42):12979-88
references: 11041863
references: J Mol Biol. 2001 May 4;308(3):477-500
references: 11327782
references: J Biol Chem. 1998 Apr 24;273(17):10515-29
references: 9553111
references: J Biol Chem. 2001 May 11;276(19):16123-36
references: 11278675
references: Nat Struct Biol. 2001 Nov;8(11):942-6
references: 11685239
references: Biochemistry. 2001 Mar 20;40(11):3295-307
references: 11258949
references: Biochemistry. 2007 Nov 13;46(45):12909-24
references: 17941646
references: Methods Enzymol. 2009;466:293-324
references: 21603116
references: Biochemistry. 1967 Jul;6(7):1948-54
references: 6049437
references: Virology. 1995 Apr 1;208(1):249-78
references: 11831707
references: J Gen Virol. 2005 Mar;86(Pt 3):589-594
references: 15722518
references: Biochemistry. 1967 Oct;6(10):3293-306
references: 6056990
references: J Biol Chem. 1997 Dec 5;272(49):30899-910
references: 9388236
references: J Mol Biol. 1969 Feb 14;39(3):539-44
references: 5357211
references: Biochemistry. 1980 May 13;19(10):2226-35
references: 7378357
references: Vet Immunol Immunopathol. 2004 Aug;100(3-4):117-34
references: 15207450
references: Methods Enzymol. 1997;278:417-44
references: 9170325
references: Biochemistry. 2009 Nov 3;48(43):10372-9
references: 19757837
references: J Mol Biol. 2006 Feb 10;356(1):121-41
references: 16337650
references: Proc Natl Acad Sci U S A. 2000 Apr 11;97(8):3987-92
references: 10760270
references: Nat Struct Mol Biol. 2004 May;11(5):457-62
references: 15077104
references: Biochemistry. 1974 Nov 19;13(24):4869-78
references: 4373039
references: Biopolymers. 1989 Sep;28(9):1637-43
references: 2775853
references: Q Rev Biophys. 1978 May;11(2):103-78
references: 353875
references: J Biol Chem. 1998 Nov 20;273(47):31021-31
references: 9813000
references: Methods Mol Biol. 2009;490:179-93
references: 19157084
references: J Am Chem Soc. 2001 Feb 28;123(8):1776-7
references: 11456786
references: Methods Enzymol. 2004;379:235-62
references: 15051361
references: Proc Natl Acad Sci U S A. 2003 Apr 29;100(9):5113-8
references: 12692307
references: Proc Natl Acad Sci U S A. 2001 Jul 17;98(15):8368-75
references: 11459977
references: Biochemistry. 2003 Oct 14;42(40):11864-78
references: 14529299
references: J Mol Biol. 2003 Mar 7;326(5):1403-12
references: 12595253
references: Chem Rev. 2006 Feb;106(2):556-606
references: 16464018
references: Proc Natl Acad Sci U S A. 2002 Jul 23;99(15):9721-6
references: 12097640
references: J Mol Biol. 2003 Jun 6;329(3):441-65
references: 12767828
references: Methods. 2001 Sep;25(1):62-77
references: 11558998
references: Methods Enzymol. 1991;208:258-90
references: 1779838
references: Anal Biochem. 1989 Nov 1;182(2):319-26
references: 2610349
references: Biochemistry. 2000 Aug 29;39(34):10454-67
references: 10956036
references: J Mol Biol. 2004 Oct 8;343(1):115-36
references: 15381424