title: Muc5b is the major polymeric mucin in mucus from thoroughbred horses with and without airway mucus accumulation.
abstract: Mucus accumulation is a feature of inflammatory airway disease in the horse and has been associated with reduced performance in racehorses. In this study, we have analysed the two major airways gel-forming mucins Muc5b and Muc5ac in respect of their site of synthesis, their biochemical properties, and their amounts in mucus from healthy horses and from horses with signs of airway mucus accumulation. Polyclonal antisera directed against equine Muc5b and Muc5ac were raised and characterised. Immunohistochemical staining of normal equine trachea showed that Muc5ac and Muc5b are produced by cells in the submucosal glands, as well as surface epithelial goblet cells. Western blotting after agarose gel electrophoresis of airway mucus from healthy horses, and horses with mucus accumulation, was used to determine the amounts of these two mucins in tracheal wash samples. The results showed that in healthy horses Muc5b was the predominant mucin with small amounts of Muc5ac. The amounts of Muc5b and Muc5ac were both dramatically increased in samples collected from horses with high mucus scores as determined visually at the time of endoscopy and that this increase also correlated with increase number of bacteria present in the sample. The change in amount of Muc5b and Muc5ac indicates that Muc5b remains the most abundant mucin in mucus. In summary, we have developed mucin specific polyclonal antibodies, which have allowed us to show that there is a significant increase in Muc5b and Muc5ac in mucus accumulated in equine airways and these increases correlated with the numbers of bacteria.
authors: Wellcome Trust Centre for Cell-Matrix Research, Faculty of Life Sciences, University of Manchester, Manchester, United Kingdom.
authors: Karine
authors: K
authors: Rousseau
authors: Jacqueline M
authors: JM
authors: Cardwell
authors: Emma
authors: E
authors: Humphrey
authors: Richard
authors: R
authors: Newton
authors: David
authors: D
authors: Knight
authors: Peter
authors: P
authors: Clegg
authors: David J
authors: DJ
authors: Thornton
references: Zentralbl Veterinarmed A. 1983 Feb;30(2):114-20
references: 6407240
references: Glycoconj J. 1996 Oct;13(5):839-47
references: 8910011
references: Equine Vet J. 1985 Mar;17(2):99-103
references: 2985380
references: Res Vet Sci. 2001 Apr;70(2):149-55
references: 11356094
references: Equine Vet J. 2004 Nov;36(7):576-82
references: 15581321
references: Anal Biochem. 1995 May 1;227(1):162-7
references: 7668377
references: Biochem J. 2002 May 15;364(Pt 1):191-200
references: 11988092
references: J Physiol. 2008 Apr 1;586(7):1977-92
references: 18258655
references: Annu Rev Physiol. 2008;70:405-29
references: 17961085
references: Am Rev Respir Dis. 1985 Nov;132(5):1066-70
references: 4062037
references: Exp Lung Res. 2002 Jun;28(4):315-32
references: 12042033
references: Annu Rev Physiol. 2008;70:459-86
references: 17850213
references: Biochem J. 2002 Feb 1;361(Pt 3):537-46
references: 11802783
references: Biotechniques. 1993 Sep;15(3):532-4, 536-7
references: 7692896
references: J Am Vet Med Assoc. 1990 May 15;196(10):1563-4
references: 2347746
references: Equine Vet J. 1995 Nov;27(6):416-21
references: 8565937
references: Biochem J. 1990 Jan 1;265(1):179-86
references: 2302164
references: Am J Respir Cell Mol Biol. 2000 Jun;22(6):649-56
references: 10837360
references: Vet Immunol Immunopathol. 2008 Sep 15;125(1-2):8-17
references: 18597857
references: Vet Clin North Am Large Anim Pract. 1979 May;1(1):17-34
references: 388830
references: J Am Vet Med Assoc. 1990 Feb 1;196(3):443-5
references: 2298674
references: J Biol Chem. 1998 Nov 13;273(46):30157-64
references: 9804771
references: Biochem J. 1998 Sep 15;334 ( Pt 3):685-93
references: 9729478
references: J Biol Chem. 2003 Jun 27;278(26):23243-50
references: 12690113
references: Equine Vet J. 2001 Jan;33(1):5-19
references: 11191611
references: J Clin Invest. 1999 Nov;104(10):1375-82
references: 10562299
references: Equine Vet J. 1993 May;25(3):208-13
references: 8508749
references: Biochem J. 1999 Mar 1;338 ( Pt 2):507-13
references: 10024529
references: J Clin Microbiol. 1984 Jan;19(1):4-7
references: 6418760
references: Equine Vet J. 1993 Jul;25(4):257-8
references: 8354206
references: Equine Vet J. 2003 May;35(3):252-7
references: 12755427
references: Equine Vet J. 2011 Nov;43(6):750-5
references: 21496098
references: Am J Respir Cell Mol Biol. 2006 Feb;34(2):135-41
references: 16195536
references: Biochem J. 1987 Aug 1;245(3):757-62
references: 3663189
references: Equine Vet J. 2001 May;33(3):256-64
references: 11352347
references: Biochem J. 1996 Aug 15;318 ( Pt 1):319-24
references: 8761488
references: Vet Rec. 1996 Sep 28;139(13):308-13
references: 8893488
references: J Biol Chem. 1997 Feb 7;272(6):3168-78
references: 9013550
references: Can Vet J. 1985 Jan;26(1):33-5
references: 17422496
references: Am J Vet Res. 2010 Jun;71(6):690-6
references: 20513186
references: Gastroenterology. 1995 Sep;109(3):723-34
references: 7657100
references: Vet Rec. 2001 Jan 27;148(4):99-104
references: 11232940
references: Biochim Biophys Acta. 2004 Feb 20;1676(3):240-50
references: 14984930
references: Am J Vet Res. 1995 May;56(5):562-7
references: 7661448
references: In Vitro Cell Dev Biol Anim. 2010 Feb;46(2):102-6
references: 19915928
references: Am J Respir Cell Mol Biol. 2007 Sep;37(3):273-90
references: 17463395
references: Exp Lung Res. 1985;9(3-4):289-308
references: 4076055
references: Methods. 2001 Dec;25(4):402-8
references: 11846609
references: Am J Respir Crit Care Med. 2008 Nov 15;178(10):1033-9
references: 18776153
references: Vet Rec. 1976 Dec 4;99(23):448-51
references: 997195
references: Am J Respir Crit Care Med. 1994 Sep;150(3):823-32
references: 8087358
references: Methods Mol Biol. 2000;125:87-96
references: 10820752
references: Respir Med. 2002 Feb;96(2):81-6
references: 11860173
references: Mol Cell Proteomics. 2006 Jan;5(1):144-56
references: 16219938
references: Biochem J. 1991 Jun 15;276 ( Pt 3):667-75
references: 2064605
references: Am J Respir Cell Mol Biol. 2011 Aug;45(2):348-58
references: 21075863
references: Am Rev Respir Dis. 1991 Mar;143(3):640-8
references: 2001077
references: J Intern Med. 2007 Jan;261(1):5-16
references: 17222164
references: Sci Transl Med. 2010 Apr 28;2(29):29ra31
references: 20427821
references: Am J Physiol Lung Cell Mol Physiol. 2007 Jun;292(6):L1396-404
references: 17293373