title: Measuring spatiotemporal dynamics of cyclic AMP signaling in real-time using FRET-based biosensors.
abstract: Cyclic AMP governs many fundamental signaling events in eukaryotic cells. Although cAMP signaling has been a major research focus for a long time, recent technological developments are revealing novel aspects of this paradigmatic pathway. In this chapter, we give an overview over current fluorescence resonance energy transfer (FRET)-based sensors for detection of cAMP dynamics, and their application in monitoring local, compartmentalized cAMP signals within living cells. A basic step-by-step protocol is given for conducting a FRET experiment in primary cells with a unimolecular cAMP sensor, which can easily be adapted to a user's specific requirements.
authors: Neuroscience and Molecular Pharmacology, Biomedical & Life Sciences, University of Glasgow, Glasgow, UK.
authors: Frank
authors: F
authors: Gesellchen
authors: Alessandra
authors: A
authors: Stangherlin
authors: Nicoletta
authors: N
authors: Surdo
authors: Anna
authors: A
authors: Terrin
authors: Anna
authors: A
authors: Zoccarato
authors: Manuela
authors: M
authors: Zaccolo