title: Robust method for high-throughput surface patterning of deformable substrates.
abstract: We describe a simple and robust method for high-throughput surface patterning of deformable substrates such as silicone rubber films covered with a thin layer of protein and cell antifouling hydrogel (PLL-g-PEG). The irradiation with deep UV (<200 nm) of PLL-g-PEG-coated rubber substrates through a synthetic quartz photomask created micropatterns over a large area of the substrate. Incubation with proteins resulted in stable patterns with high feature resolution. RPE1 cells seeded on fibronectin patterns were constrained for days even after stretching. We also propose the crossbow feature as an interesting example allowing the stretching of normalized stress fibers.
authors: Systems Biology for Cell Division and Cell Polarity, Institut Curie, CNRS, UMR 144, 26 rue d'Ulm, 75005 Paris, France. ammar.azioune@u-bordeaux2.fr
authors: Ammar
authors: A
authors: Azioune
authors: Nicolas
authors: N
authors: Carpi
authors: Jenny
authors: J
authors: Fink
authors: Mohamed M
authors: MM
authors: Chehimi
authors: Damien
authors: D
authors: Cuvelier
authors: Matthieu
authors: M
authors: Piel