title: A highly sensitive and rapid organophosphate biosensor based on enhancement of CdS-decorated graphene nanocomposite.
abstract: This work reports a rapid and sensitive organophosphates (OPs) amperometric biosensor based on acetylcholinesterase (AChE) immobilized on CdS-decorated graphene (CdS-G) nanocomposite. The as-prepared biosensor shows high affinity to acetylthiocholine (ATCl) with a Michaelis-Menten constant (K(m)) value of 0.24 mM. A rapid inhibition time (2 min) is obtained due to the integration of the CdS-G nanocomposite. Based on the inhibition of OPs on the enzymatic activity of the immobilized AChE, and used carbaryl as the model compound, the resulting biosensor exhibits excellent performance for OPs detection including good reproducibility, acceptable stability, and a reliable linear relationship between the inhibition and log[carbaryl] from 2 ng mL⁻¹ up to 2 μg mL⁻¹ with a detection limit of 0.7 ng mL⁻¹,which provides a new promising tool for analysis of enzyme inhibitors.
authors: Key Laboratory of Modern Agriculture Equipment and Technology, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, China. wangkun@ujs.edu.cn
authors: Kun
authors: K
authors: Wang
authors: Qian
authors: Q
authors: Liu
authors: Lina
authors: L
authors: Dai
authors: Jiajia
authors: J
authors: Yan
authors: Chang
authors: C
authors: Ju
authors: Baijing
authors: B
authors: Qiu
authors: Xiangyang
authors: X
authors: Wu