title: Differential effects of thiopeptide and orthosomycin antibiotics on translational GTPases.
abstract: The ribosome is a major target in the bacterial cell for antibiotics. Here, we dissect the effects that the thiopeptide antibiotics thiostrepton (ThS) and micrococcin (MiC) as well as the orthosomycin antibiotic evernimicin (Evn) have on translational GTPases. We demonstrate that, like ThS, MiC is a translocation inhibitor, and that the activation by MiC of the ribosome-dependent GTPase activity of EF-G is dependent on the presence of the ribosomal proteins L7/L12 as well as the G' subdomain of EF-G. In contrast, Evn does not inhibit translocation but is a potent inhibitor of back-translocation as well as IF2-dependent 70S-initiation complex formation. Collectively, these results shed insight not only into fundamental aspects of translation but also into the unappreciated specificities of these classes of translational inhibitors.
authors: Gene Center and Department of Biochemistry, University of Munich, Germany.
authors: Aleksandra
authors: A
authors: Mikolajka
authors: Hanqing
authors: H
authors: Liu
authors: Yuanwei
authors: Y
authors: Chen
authors: Agata L
authors: AL
authors: Starosta
authors: Viter
authors: V
authors: Márquez
authors: Marina
authors: M
authors: Ivanova
authors: Barry S
authors: BS
authors: Cooperman
authors: Daniel N
authors: DN
authors: Wilson
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