title: Effect of sample preparation methods on the D,L-enantiomer ratio of extracted selenomethionine.
abstract: Effects of the two most widespread sample preparation techniques on the D,L-enantiomer ratio of extracted selenomethionine were monitored through the analysis of the certified reference material selenium-enriched yeast and the isolated protein fraction of high selenium monkeypot nut. The extracted selenomethionine (SeMet) fractions were orthogonally cleaned up with anion exchange chromatography before carrying out the enantiomer-specific detection to increase the robustness and the efficiency of the subsequent o-phthal-aldehyde and n-isobutyril-cysteine-based derivatisation process and reversed phase-high-performance liquid chromatography-inductively coupled plasma mass spectroscopy (ICP-MS) detection. The two techniques, namely methanesulphonic acid (MSA) based digestion and proteolytic digestion with protease XIV, resulted in significantly different ratio of D,L-selenomethionine with the final results of 2.2-2.7% and 0.5-0.6% of D-SeMet, respectively. The study revealed significant differences in the ICP-MS-related sensitivity of the derivatised selenomethionine enantiomers, which calls attention to the quantification of this selenoamino acid after MSA hydrolysis.
authors: Department of Applied Chemistry, Corvinus University of Budapest, Budapest, Hungary.
authors: Orsolya
authors: O
authors: Egressy-Molnár
authors: Andrea
authors: A
authors: Vass
authors: Anikó
authors: A
authors: Németh
authors: Juan F
authors: JF
authors: García-Reyes
authors: Mihály
authors: M
authors: Dernovics