title: Inhibition of fibroblast growth factor receptor 1: influence on tympanic membrane wound healing in rats.
abstract: An animal model of chronic tympanic membrane (TM) perforation is needed for experiments on supporting healing of TM perforations. The basic fibroblast growth factor is important in TM wound healing. The object of this study was to investigate the efficacy of fibroblast growth factor receptor 1 (FGFR1) inhibition to arrest wound healing of experimental TM perforation. Bilateral instrumental myringotomies were performed in 12 rats. A specific inhibitor of the FGFR1 tyrosine kinase (SU5402) was applied to the left TM (2 mg/ml) and to the right TM (10 mg/ml) of each animal daily for 12 consecutive days. Thereafter, TMs were observed weekly for a total of 30 days. TM healing was delayed in a dose-dependent manner. We observed differences in the histologic parameters between both groups. SU 5402 is a strong inhibitor of TM healing but seems not to be suitable to create a chronic TM perforation in rat.
authors: Department of Otorhinolaryngology and Head and Neck Surgery, Ernst Moritz Arndt University, Walther Rathenau Str 43-45, 17475 Greifswald, Germany. holger.kaftan@uni-greifswald.de
authors: Holger
authors: H
authors: Kaftan
authors: Lars
authors: L
authors: Reuther
authors: Bärbel
authors: B
authors: Miehe
authors: Werner
authors: W
authors: Hosemann
authors: Achim
authors: A
authors: Beule
references: Science. 1997 May 9;276(5314):955-60
references: 9139660
references: ORL J Otorhinolaryngol Relat Spec. 1995 Jan-Feb;57(1):28-32
references: 7700606
references: ORL J Otorhinolaryngol Relat Spec. 1991;53(1):32-6
references: 2008292
references: J Mol Histol. 2011 Feb;42(1):15-25
references: 21072681
references: Oncogene. 2004 Dec 9;23(57):9201-11
references: 15516981
references: Wound Repair Regen. 2008 May-Jun;16(3):364-9
references: 18471254
references: J Clin Invest. 2009 Mar;119(3):512-23
references: 19197140
references: Acta Otolaryngol. 1995 Jan;115(1):50-4
references: 7762385
references: HNO. 2004 Aug;52(8):714-9
references: 15309252
references: Wound Repair Regen. 2008 Sep-Oct;16(5):585-601
references: 19128254
references: Otol Neurotol. 2010 Jan;31(1):118-21
references: 19940793
references: Laryngoscope. 1993 Jul;103(7):804-9
references: 8341107
references: J Invest Dermatol. 2006 Jun;126(6):1403-9
references: 16543894
references: Clin Cancer Res. 2005 Apr 1;11(7):2702-12
references: 15814652
references: Neuroscience. 2007 Nov 30;150(1):202-11
references: 17905520
references: Am J Physiol Endocrinol Metab. 2009 Jan;296(1):E121-31
references: 18940940
references: Endocr Relat Cancer. 2000 Sep;7(3):165-97
references: 11021964
references: Wound Repair Regen. 2006 Jul-Aug;14(4):453-6
references: 16939573
references: Growth Factors. 2010 Aug;28(4):286-92
references: 20166887
references: Otolaryngol Head Neck Surg. 1995 Oct;113(4):375-9
references: 7567007
references: Mol Cancer Ther. 2008 Oct;7(10):3408-19
references: 18852144
references: Laryngoscope. 1993 Mar;103(3):312-8
references: 7680087
references: HNO. 2006 Dec;54(12):943-6
references: 16622695
references: Arch Otorhinolaryngol. 1977 Mar 8;215(1):55-9
references: 577139
references: Cytokine Growth Factor Rev. 2005 Apr;16(2):139-49
references: 15863030
references: J Biol Chem. 1999 Dec 3;274(49):34785-94
references: 10574949