title: Identification and characterization of a neutralizing-epitope-containing spike protein fragment in turkey coronavirus.
abstract: Little is known about the neutralizing epitopes in turkey coronavirus (TCoV). The spike (S) protein gene of TCoV was divided into 10 fragments to identify the antigenic region containing neutralizing epitopes. The expression and antigenicity of S fragments was confirmed by immunofluorescence antibody (IFA) assay using an anti-histidine monoclonal antibody or anti-TCoV serum. Polyclonal antibodies raised against expressed S1 (amino acid position 1 to 573 from start codon of S protein), 4F/4R (482-678), 6F/6R (830-1071), or Mod4F/Epi4R (476-520) S fragment recognized native S1 protein and TCoV in the intestines of TCoV-infected turkey embryos. Anti-TCoV serum reacted with recombinant 4F/4R, 6F/6R, and Mod4F/Epi4R in a western blot. The results of a virus neutralization assay indicated that the carboxyl terminal region of the S1 protein (Mod4F/Epi4R) or the combined carboxyl terminal S1 and amino terminal S2 protein (4F/4R) possesses the neutralizing epitopes, while the S2 fragment (6F/6R) contains antigenic epitopes but not neutralizing epitopes.
authors: Department of Comparative Pathobiology, Purdue University, 406 South University Street, West Lafayette, IN 47907, USA.
authors: Yi-Ning
authors: YN
authors: Chen
authors: Ching Ching
authors: CC
authors: Wu
authors: Tsang Long
authors: TL
authors: Lin
references: Microbiol Immunol. 2009 Feb;53(2):75-82
references: 19291090
references: Virology. 2010 Mar 1;398(1):98-108
references: 20022075
references: Avian Pathol. 2009 Apr;38(2):181-6
references: 19322719
references: Adv Exp Med Biol. 1998;440:69-75
references: 9782266
references: Intervirology. 2006;49(4):230-8
references: 16491018
references: J Virol. 2005 Mar;79(6):3289-96
references: 15731223
references: Mol Immunol. 1989 Jan;26(1):7-15
references: 2467199
references: J Clin Microbiol. 2004 Apr;42(4):1570-6
references: 15071006
references: Exp Biol Med (Maywood). 2009 Oct;234(10):1117-27
references: 19546349
references: J Virol. 1990 Nov;64(11):5367-75
references: 2170676
references: Virology. 1987 Dec;161(2):511-9
references: 2446423
references: Avian Dis. 1986 Apr-Jun;30(2):319-26
references: 3015116
references: J Vet Med Sci. 1999 Aug;61(8):935-8
references: 10487234
references: Clin Diagn Lab Immunol. 2001 Mar;8(2):297-302
references: 11238212
references: Avian Pathol. 2001 Aug;30(4):355-68
references: 19184921
references: Adv Exp Med Biol. 1995;380:359-65
references: 8830508
references: J Virol. 1997 Nov;71(11):8860-3
references: 9343248
references: Arch Virol. 2005 Sep;150(9):1813-31
references: 15868095
references: Arch Virol. 1994;138(1-2):117-34
references: 7980002
references: Virology. 2007 Mar 15;359(2):362-70
references: 17055551
references: Avian Dis. 1997 Jul-Sep;41(3):583-90
references: 9356703
references: Poult Sci. 2001 Oct;80(10):1416-24
references: 11599699
references: Vet Microbiol. 2008 Sep 18;131(1-2):73-81
references: 18400422
references: Adv Exp Med Biol. 1990;276:205-10
references: 1715662
references: Biochem Biophys Res Commun. 2009 Dec 18;390(3):1056-60
references: 19861118
references: Vaccine. 2003 Jun 20;21(21-22):2730-6
references: 12798610
references: J Comb Chem. 2005 Sep-Oct;7(5):648-56
references: 16153058
references: Microbes Infect. 2008 Jul;10(8):908-15
references: 18606245
references: Trends Genet. 2000 Jun;16(6):276-7
references: 10827456
references: Clin Vaccine Immunol. 2008 Dec;15(12):1839-44
references: 18971306
references: J Virol. 2004 Jul;78(13):7217-26
references: 15194798
references: J Gen Virol. 2005 Jan;86(Pt 1):211-215
references: 15604448
references: J Virol. 2004 Jul;78(13):6938-45
references: 15194770
references: Virology. 2000 May 25;271(1):182-96
references: 10814583
references: Arch Virol. 2009;154(7):1117-24
references: 19543689
references: Hum Vaccin. 2008 Nov-Dec;4(6):453-6
references: 18535407
references: J Virol. 2009 Aug;83(15):7411-21
references: 19439480
references: J Virol. 1994 Sep;68(9):5403-10
references: 7520090
references: Avian Dis. 2001 Jan-Mar;45(1):136-43
references: 11332474
references: Int Rev Immunol. 2006 May-Aug;25(3-4):99-123
references: 16818367
references: Biochem Biophys Res Commun. 2003 Oct 10;310(1):78-83
references: 14511651
references: Arch Virol. 1997;142(11):2249-56
references: 9672590
references: J Virol. 1994 Dec;68(12):8008-16
references: 7525985
references: Avian Dis. 2002 Apr-Jun;46(2):466-72
references: 12061660
references: Virology. 2009 Oct 10;393(1):144-50
references: 19683779
references: J Immunol. 2004 Sep 15;173(6):4050-7
references: 15356154
references: Vaccine. 2005 Oct 10;23(42):4959-68
references: 15993989
references: J Gen Virol. 1993 Oct;74 ( Pt 10):2061-9
references: 8409930
references: Vet Microbiol. 2002 Jan 3;84(1-2):179-86
references: 11731170
references: Avian Pathol. 2007 Feb;36(1):29-33
references: 17364507
references: Nature. 2004 Apr 1;428(6982):561-4
references: 15024391
references: J Virol. 2003 Aug;77(16):8801-11
references: 12885899
references: J Feline Med Surg. 2004 Apr;6(2):111-8
references: 15123156
references: FEBS Lett. 1990 Dec 10;276(1-2):172-4
references: 1702393
references: Infect Immun. 2003 Aug;71(8):4664-73
references: 12874347
references: Avian Dis. 2002 Oct-Dec;46(4):831-8
references: 12495043