title: Characterization of Syrian hamster adapted prions derived from L-type and C-type bovine spongiform encephalopathies.
abstract: Atypical forms of bovine spongiform encephalopathy (BSE) may be caused by different prions from classical BSE (C-BSE). In this study, we examined the susceptibility of mice overexpressing mouse and hamster chimeric prion protein (PrP) to L-type atypical BSE (L-BSE). None of the transgenic mice showed susceptibility to L-BSE, except mice overexpressing hamster PrP. We also examined the transmission properties of L-BSE in hamsters. The incubation period of hamsters intracerebrally inoculated with L-BSE was 576.8 days, and that of the subsequent passage was decreased to 208 days. Although the lesion and glycoform profiles and relative proteinase K resistant core fragment of the abnormal isoform of PrP (PrPcore) of L-BSE were similar to that of C-BSE, the deposition of the abnormal isoform of PrP (PrPSc) and the molecular weight of PrPcore of L-BSE was different from than that of C-BSE. In hamster models, some prion strain characteristics of L-BSE were indistinguishable from those of C-BSE.
authors: Prion Disease Research Center, National Institute of Animal Health, Tsukuba, Ibaraki, Japan.
authors: Yujing
authors: Y
authors: Shu
authors: Kentaro
authors: K
authors: Masujin
authors: Hiroyuki
authors: H
authors: Okada
authors: Yoshifumi
authors: Y
authors: Iwamaru
authors: Morikazu
authors: M
authors: Imamura
authors: Yuichi
authors: Y
authors: Matsuura
authors: Shirou
authors: S
authors: Mohri
authors: Takashi
authors: T
authors: Yokoyama
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