title: In vivo imaging of lymph node migration of MNP- and (111)In-labeled dendritic cells in a transgenic mouse model of breast cancer (MMTV-Ras).
abstract: OBJECTIVE
  The authors present a protocol for the in vivo evaluation, using different imaging techniques, of lymph node (LN) homing of tumor-specific dendritic cells (DCs) in a murine breast cancer model.
  
  
  METHODS
  Bone marrow DCs were labeled with paramagnetic nanoparticles (MNPs) or (111)In-oxine. Antigen loading was performed using tumor lysate. Mature DCs were injected into the footpads of transgenic tumor-bearing mice (MMTV-Ras) and DC migration was tracked by magnetic resonance imaging (MRI) and single-photon emission computed tomography (SPECT). Ex vivo analyses were performed to validate the imaging data.
  
  
  RESULTS
  DC labeling, both with MNPs and with (111)In-oxine, did not affect DC phenotype or functionality. MRI and SPECT allowed the detection of iron and (111)In in both axillary and popliteal LNs. Immunohistochemistry and γ-counting revealed the presence of DCs in LNs.
  
  
  CONCLUSIONS
  MRI and SPECT imaging, by allowing in vivo dynamic monitoring of DC migration, could further the development and optimization of efficient anti-cancer vaccines.
authors: Department of Biomedical Sciences and Technologies, Section of Radiological Sciences, University of Milan, via di Rudinì 8, 20142 Milan, Italy.
authors: Cristina
authors: C
authors: Martelli
authors: Manuela
authors: M
authors: Borelli
authors: Luisa
authors: L
authors: Ottobrini
authors: Veronica
authors: V
authors: Rainone
authors: Anna
authors: A
authors: Degrassi
authors: Micaela
authors: M
authors: Russo
authors: Umberto
authors: U
authors: Gianelli
authors: Silvano
authors: S
authors: Bosari
authors: Carlo
authors: C
authors: Fiorini
authors: Daria
authors: D
authors: Trabattoni
authors: Mario
authors: M
authors: Clerici
authors: Giovanni
authors: G
authors: Lucignani
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