title: A comparison of lower genital tract glycogen and lactic acid levels in women and macaques: implications for HIV and SIV susceptibility.
abstract: Understanding factors that affect heterosexual transmission of HIV in women is of great importance. Lactobacilli in the lower genital tract of women utilize glycogen in vaginal epithelial cells as an energy source and produce lactic acid. The resultant vaginal acidity is believed to provide protection against HIV infection. Conversely, bacterial vaginosis (BV) is characterized by less lactic acid and a higher pH, and is associated with increased susceptibility to HIV infection. Because vaginal infection of macaques with simian immunodeficiency virus (SIV) or simian-human immunodeficiency virus (SHIV) is used as a model to study HIV sexual transmission, and because previous studies have shown a paucity of lactobacilli in rhesus macaques' lower genital tract, we compared lactic acid and glycogen levels in the genital fluid of rhesus and pigtail macaques with levels found in humans. The levels of lactic acid were lower in both rhesus (median=1.2 mol lactate/mg protein) and pigtail macaques (median=0.7 mol/mg) compared to women with healthy genital microbiota (median=4.2 mol/mg). Glycogen levels were significantly lower in both rhesus (median=0.004 μg glycogen/μg protein) and pigtail macaques (median=0 μg/μg) than in women (median=0.2 μg/μg). No significant differences in glycogen or lactate levels were observed comparing longitudinally collected samples from cycling pigtail macaques. These data show that the previously reported scarcity of lactobacilli in macaques correlates with low glycogen and lactic acid levels. These findings have important implications for studies of vaginal infection of macaques with SIV or SHIV and further our understanding of how the bacterial microbiota influences HIV infection.
authors: Department of Immunology/Microbiology, Rush University Medical Center, Chicago, Illinois 60612, USA.
authors: Paria
authors: P
authors: Mirmonsef
authors: Douglas
authors: D
authors: Gilbert
authors: Ronald S
authors: RS
authors: Veazey
authors: Jing
authors: J
authors: Wang
authors: Sabrina R
authors: SR
authors: Kendrick
authors: Gregory T
authors: GT
authors: Spear
references: Hum Reprod. 2001 Sep;16(9):1809-13
references: 11527880
references: Antimicrob Agents Chemother. 2007 May;51(5):1608-15
references: 17353240
references: FEMS Microbiol Rev. 2010 Nov;34(6):952-85
references: 20412306
references: Am J Primatol. 2005 Sep;67(1):159-74
references: 16163716
references: Scand J Infect Dis Suppl. 1983;40:31-5
references: 6582587
references: Sex Transm Dis. 2006 Nov;33(11):691-5
references: 16641825
references: Antimicrob Agents Chemother. 2008 Dec;52(12):4448-54
references: 18838587
references: Curr HIV/AIDS Rep. 2007 Feb;4(1):22-8
references: 17338857
references: Fertil Steril. 1971 Jan;22(1):64-8
references: 5538756
references: Am J Obstet Gynecol. 1993 Aug;169(2 Pt 2):455-9
references: 8357044
references: Sex Transm Dis. 2004 May;31(5):290-6
references: 15107631
references: Microbes Infect. 1999 Apr;1(4):285-92
references: 10602662
references: AIDS Res Hum Retroviruses. 2010 Feb;26(2):193-200
references: 20156101
references: AIDS. 2005 Oct 14;19(15):1595-9
references: 16184028
references: AIDS Res Ther. 2007 Oct 22;4:25
references: 17953761
references: AIDS. 2008 Jul 31;22(12):1493-501
references: 18614873
references: Clin Infect Dis. 2003 Aug 1;37(3):319-25
references: 12884154
references: Biol Reprod. 1972 Aug;7(1):9-14
references: 4340454
references: J Infect Dis. 2007 Dec 1;196(11):1692-7
references: 18008255
references: J Ultrastruct Res. 1983 Jan;82(1):1-18
references: 6848769
references: Infect Immun. 1999 Oct;67(10):5170-5
references: 10496892
references: Sex Transm Dis. 2008 Sep;35(9):791-6
references: 18607314