title: Evaluation of a risk-screening questionnaire to detect equine lung inflammation: results of a large field study.
abstract: BACKGROUND
  The diagnosis of equine recurrent airway obstruction (RAO) and inflammatory airway disease (IAD) is based on clinical signs and increased inflammatory cell percentages in the bronchoalveolar lavage (BAL) fluid. Since a BAL is an invasive procedure, a risk-screening questionnaire (RSQ) would be a valuable screening tool for lung inflammation.
  
  
  OBJECTIVE
  To evaluate the accuracy of a RSQ to detect lower airway inflammation (LAI) in a large population of horses.
  
  
  METHODS
  A standardised BAL was performed in the field on 167 horses in Alberta, Canada. Horses were separated into 3 categories: 1) BAL normal; 2) BAL mild to moderate LAI (MLAI), and 3) BAL severe LAI (SLAI). The horse owners were asked to complete a RSQ. The RSQ scores were compared to the BAL results to determine the likelihood of a horse having MLAI, SLAI or no LAI.
  
  
  RESULTS
  Based on BAL cytology, 28 (17%) horses were normal and 139 (83%) were abnormal, with 110 (66%) showing MLAI and 29 (17%) SLAI. Horses with SLAI and MLAI had a mean RSQ score of 0.95 and 0.70, respectively, compared to 0.60 for normal BAL horses. Horses with SLAI showed more clinical signs than normal and MLAI horses. The sensitivity and negative predictive values of the RSQ for detecting SLAI using a cut-off score of 0.87, were excellent at 0.90 (95%CI 0.73-0.98) and 0.96 (95%CI 0.82-1.00). Questions on the clinical signs typically found in RAO cases differed significantly between horses with BAL SLAI and those with BAL normal.
  
  
  CONCLUSIONS
  Prevalence of MLAI was high in this population. Although the RSQ did not allow differentiating normal horses from horses with MLAI, it has a high sensitivity to detect horses with SLAI and is therefore a good screening tool for SLAI.
authors: Department of Production Animal Health and Veterinary Clinical and Diagnostic Sciences, Faculty of Veterinary Medicine, University of Calgary, Alberta, Canada.
authors: A J
authors: AJ
authors: Wasko
authors: H W
authors: HW
authors: Barkema
authors: J
authors: J
authors: Nicol
authors: N
authors: N
authors: Fernandez
authors: N
authors: N
authors: Logie
authors: R
authors: R
authors: Léguillette