title: How to screen non-viral gene delivery systems in vitro?
abstract: Screening of new gene delivery candidates regarding transfection efficiency and toxicity is usually performed by reading out transgene expression levels relative to a reference formulation after in vitro transfection. However, over the years and among different laboratories, this screening has been performed in a variety of cell lines, using a variety of conditions and read-out systems, and by comparison to a variety of reference formulations. This makes a direct comparison of results difficult, if not impossible. Reaching a consensus would enable placing new results into context of previous findings and estimate the overall contribution to the improvement of non-viral gene delivery. In this paper we illustrate the sensitivity of transfection outcomes on testing conditions chosen, and propose a screening protocol with the aim of standardization within the field.
authors: Department of Pharmaceutics, Utrecht Institute of Pharmaceutical Sciences, Utrecht University, 3584 CG, Utrecht, The Netherlands.
authors: Ethlinn V B
authors: EV
authors: van Gaal
authors: Roel
authors: R
authors: van Eijk
authors: Ronald S
authors: RS
authors: Oosting
authors: Robbert Jan
authors: RJ
authors: Kok
authors: Wim E
authors: WE
authors: Hennink
authors: Daan J A
authors: DJ
authors: Crommelin
authors: Enrico
authors: E
authors: Mastrobattista