title: Melatonin protects lung mitochondria from aging.
abstract: We assessed whether melatonin administration would prevent the hyperoxidative status that occurs in lung mitochondria with age. Mitochondria from lungs of male and female senescent prone mice at 5 and 10 months of age were studied. Age-dependent mitochondrial oxidative stress was evaluated by measuring the levels of lipid peroxidation and nitrite, glutathione/glutathione disulfide ratio, and glutathione peroxidase and reductase activities. Mitochondrial respiratory chain and oxidative phosphorylation capability were also measured. Age induces a significant oxidative/nitrosative status in lung mitochondria, which exhibited a significantly reduced activity of the respiratory chain and ATP production. These manifestations of age were more pronounced in males than in females. After 9 months of melatonin administration in the drinking water, the hyperoxidative status and functional deficiency of aged lung mitochondria were totally counteracted, and had increased ATP production. The beneficial effects of melatonin were generally similar in both mice genders. Thus, melatonin administration, as a single therapy, maintained fully functioning lung mitochondria during aging, a finding with important consequences in the pathophysiology of lung aging. In view of these data melatonin, the production of which decreases with age, should be considered a preventive therapy against the hyperoxidative status of the aged lungs, and its use may lead to the avoidance of respiratory complications in the elderly.
authors: Instituto de Biotecnología, Centro de Investigación Biomédica, Parque Tecnológico de Ciencias de la Salud, Universidad de Granada, Spain.
authors: Darío
authors: D
authors: Acuña-Castroviejo
authors: Miguel
authors: M
authors: Carretero
authors: Carolina
authors: C
authors: Doerrier
authors: Luis C
authors: LC
authors: López
authors: Laura
authors: L
authors: García-Corzo
authors: Jesús A
authors: JA
authors: Tresguerres
authors: Germaine
authors: G
authors: Escames
references: FASEB J. 1999 Sep;13(12):1537-46
references: 10463945
references: J Pineal Res. 2001 Mar;30(2):65-74
references: 11270481
references: Can J Anaesth. 2006 Dec;53(12):1244-57
references: 17142659
references: Exp Gerontol. 2006 Nov;41(11):1165-73
references: 17049787
references: FASEB J. 1996 Jun;10(8):882-90
references: 8666165
references: Curr Top Med Chem. 2011;11(2):221-40
references: 21244359
references: Neurosci Lett. 2000 Jan 7;278(1-2):105-8
references: 10643812
references: Antioxid Redox Signal. 2010 Aug 1;13(3):269-78
references: 20059401
references: Free Radic Biol Med. 2002 Dec 1;33(11):1440-50
references: 12446201
references: Proc Am Thorac Soc. 2005;2(5):433-9
references: 16322596
references: Biochem Biophys Res Commun. 1998 Dec 30;253(3):614-20
references: 9918777
references: Mech Ageing Dev. 2002 Nov;123(12):1553-61
references: 12470893
references: J Pineal Res. 2007 Jan;42(1):28-42
references: 17198536
references: J Pineal Res. 2007 Apr;42(3):272-9
references: 17349026
references: Exp Gerontol. 1998 Nov-Dec;33(7-8):759-72
references: 9951620
references: Am J Physiol Lung Cell Mol Physiol. 2009 Jan;296(1):L14-29
references: 18931054
references: J Pineal Res. 2010 Jan;48(1):20-38
references: 19925580
references: J Immunol. 2003 Jul 15;171(2):955-63
references: 12847267
references: J Pineal Res. 2010 May;48(4):297-310
references: 20433638
references: Eur Respir J. 1990 Oct;3(9):1029-36
references: 2289550
references: Free Radic Biol Med. 1999 Nov;27(9-10):922-35
references: 10569625
references: Rejuvenation Res. 2010 Apr-Jun;13(2-3):292-7
references: 20345280
references: J Pineal Res. 2000 Nov;29(4):193-200
references: 11068941
references: J Pineal Res. 2006 Mar;40(2):153-7
references: 16441552
references: Am J Physiol Cell Physiol. 2006 Dec;291(6):C1172-82
references: 16807301
references: Neurochem Int. 2002 Jun;40(7):593-601
references: 11900854
references: Cell. 1994 Sep 23;78(6):915-8
references: 7522969
references: Exp Gerontol. 2008 Aug;43(8):749-56
references: 18485648
references: Free Radic Biol Med. 2011 Jan 1;50(1):166-78
references: 21047551
references: Am J Physiol Endocrinol Metab. 2001 Oct;281(4):E803-8
references: 11551858
references: FASEB J. 2001 Oct;15(12):2294-6
references: 11511530
references: J Pineal Res. 2009 Sep;47(2):109-126
references: 19573038
references: Inhal Toxicol. 2000;12 Suppl 4:227-46
references: 12881894
references: Clin Interv Aging. 2006;1(3):253-60
references: 18046878
references: Am J Respir Crit Care Med. 1994 Jul;150(1):238-44
references: 8025756
references: Methods. 2002 Apr;26(4):307-16
references: 12054921
references: Free Radic Biol Med. 1998 Sep;25(4-5):392-403
references: 9741578
references: Anal Biochem. 1976 Jul;74(1):214-26
references: 962076
references: Int J Biochem Cell Biol. 2006 Feb;38(2):267-78
references: 16223598
references: Proc Natl Acad Sci U S A. 1981 Dec;78(12):7764-8
references: 6950416
references: Int J Biochem Cell Biol. 2002 Apr;34(4):348-57
references: 11854034
references: J Endocrinol. 2007 Sep;194(3):637-43
references: 17761903
references: FEBS J. 2007 Apr;274(8):2135-47
references: 17371545
references: Respir Res. 2008 Feb 28;9:24
references: 18307797
references: Cell. 2005 Feb 25;120(4):437-47
references: 15734677
references: J Neurochem. 2001 May;77(3):804-11
references: 11331409
references: Free Radic Biol Med. 2003 Sep 15;35(6):626-35
references: 12957655
references: Biochim Biophys Acta. 2004 Jul 23;1658(1-2):44-9
references: 15282173
references: FASEB J. 2003 May;17(8):932-4
references: 12670878
references: Physiol Rev. 2002 Jan;82(1):47-95
references: 11773609
references: J Pineal Res. 2009 Sep;47(2):192-200
references: 19573039
references: J Biol Chem. 2010 Dec 17;285(51):39646-54
references: 20937819
references: Front Biosci. 2007 Jan 01;12:947-63
references: 17127351
references: Neurobiol Aging. 1999 Mar-Apr;20(2):105-10
references: 10537019
references: J Pineal Res. 2009 Mar;46(2):188-98
references: 19054298
references: Methods Enzymol. 2002;348:93-112
references: 11885298
references: J Cell Biochem. 2008 Oct 15;105(3):641-7
references: 18759327
references: Apoptosis. 2007 May;12(5):877-85
references: 17294083
references: FASEB J. 2000 Sep;14(12):1677-9
references: 10973915
references: Crit Rev Biochem Mol Biol. 2009 Jul-Aug;44(4):175-200
references: 19635037
references: Exp Gerontol. 1998 Jan-Mar;33(1-2):113-26
references: 9467721
references: Rejuvenation Res. 2006 Summer;9(2):169-81
references: 16706639
references: Free Radic Biol Med. 2002 Sep 1;33(5):575-86
references: 12208343
references: Brain Res Brain Res Protoc. 1999 Dec;4(3):314-21
references: 10592340
references: Am J Physiol Endocrinol Metab. 2010 Dec;299(6):E879-86
references: 20858748
references: Methods Enzymol. 1990;186:407-21
references: 2233308
references: J Biol Chem. 1951 Nov;193(1):265-75
references: 14907713
references: J Pineal Res. 2009 Jan;46(1):106-14
references: 19090913
references: Respir Physiol Neurobiol. 2010 Dec 31;174(3):265-71
references: 20883826
references: Biochim Biophys Acta. 2001 Mar 1;1504(1):46-57
references: 11239484
references: J Biol Chem. 1988 Nov 25;263(33):17205-8
references: 3053703