title: A cascade of protein kinase C isozymes promotes cytoskeletal polarization in T cells.
abstract: Polarization of the T cell microtubule-organizing center (MTOC) toward the antigen-presenting cell (APC) is driven by the accumulation of diacylglycerol (DAG) at the immunological synapse (IS). The mechanisms that couple DAG to the MTOC are not known. By single-cell photoactivation of the T cell antigen receptor (TCR), we found that three distinct isoforms of protein kinase C (PKC) were recruited by DAG to the IS in two steps. PKC-ɛ and PKC-η accumulated first in a broad region of membrane, whereas PKC-θ arrived later in a smaller zone. Functional experiments indicated that PKC-θ was required for MTOC reorientation and that PKC-ɛ and PKC-η operated redundantly to promote the recruitment of PKC-θ and subsequent polarization responses. Our results establish a previously uncharacterized role for PKC proteins in T cell polarity.
authors: Immunology Program, Memorial Sloan-Kettering Cancer Center, New York, New York, USA.
authors: Emily J
authors: EJ
authors: Quann
authors: Xin
authors: X
authors: Liu
authors: Grégoire
authors: G
authors: Altan-Bonnet
authors: Morgan
authors: M
authors: Huse
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