title: Assisted hatching of poor-quality bovine embryos increases pregnancy success rate after embryo transfer.
abstract: Embryos of good, fair and poor quality were collected from superovulated cows and subjected to zona cutting (ZC) treatment using a needle under either an inverted microscope or a stereomicroscope. One (single transfer) or 2 (twin transfer) embryos with or without prior ZC treatment were transferred nonsurgically to recipients. Without the ZC treatment, lower embryonic quality resulted in lower pregnancy success rates. However, the ZC treatment increased the pregnancy success rate following transfer of poor-quality embryos, but not the pregnancy rate after transfer of good- or fair-quality embryos. No differences were observed between the pregnancy success rates after the transfer of embryos treated under the inverted microscope and those after transfer of embryos treated under the stereomicroscope, and this was the same after single and twin transfer. Moreover, ZC treatment of embryos prior to transfer did not result in an increased abortion rate, irrespective of the number of transferred embryos. In conclusion, ZC treatment improves pregnancy success rates following transfer of poor-quality embryos. Moreover, the results indicate that ZC treatment by using a stereomicroscope is practical for on-farm application.
authors: Nagasaki Prefectural Livestock Experiment Station, Nagasaki, Japan.
authors: Atsushi
authors: A
authors: Taniyama
authors: Yasutaka
authors: Y
authors: Watanabe
authors: Youji
authors: Y
authors: Nishino
authors: Tetsurou
authors: T
authors: Inoue
authors: Yasuho
authors: Y
authors: Taura
authors: Mitsuhiro
authors: M
authors: Takagi
authors: Chikara
authors: C
authors: Kubota
authors: Takeshige
authors: T
authors: Otoi