title: [Optimization of a HPLC determination method for Evodia rutaecarpa].
abstract: A HPLC method for determination of limonin, evodiamine and rutaecarpine in Evodia rutaecarpa was optimized. The mobile phase was [acetonitrile-tetrahydrofuran (25: 15)] -0.02% H3 PO4 (35:65). The detection wavelength was 220 nm and the flow rate was 1.0 mL x min(-1). Limonin, evodiamine and rutaecarpine were all well separated from other substances and their UV spectrums were essentially the same to the standards . The liner ranges of limonin, evodiamine and rutaecarpine were 0.196 8-3.936, 0.153 6-3.072, 0.097 4-1.948 microg. The average recoveries were 97.8%, 100.7% and 98.4%. RSD were 1.7%, 1.3% and 1.1% (n = 6). The method of this article is accurate, reproducible and can be used to enhance the quality control of E. rutaecarpa.
authors: Sichuan Academy of Chinese Medicine Sciences, Chengdu 610041, China.
authors: Zhifang
authors: Z
authors: Huang
authors: Jinhai
authors: J
authors: Yi
authors: Yan
authors: Y
authors: Wu
authors: Yunhua
authors: Y
authors: Liu
authors: Yan
authors: Y
authors: Chen
authors: Yuhong
authors: Y
authors: Liu