title: The effect of oxycholesterols on thermo-induced membrane dynamics.
abstract: The effect of temperature change(s) on the dynamics of giant unilamellar vesicles containing oxidized and non-oxidized cholesterol was investigated and characterized. We have demonstrated that (i) major cholesterol auto-oxidation products, 7β-hydroxycholesterol (7β) and 7-ketocholesterol (7keto), rendered vesicles more responsive to temperature changes; (ii) 7keto imparted greater thermo-induced membrane dynamics than 7β; (iii) 7β and 7keto vesicles synergistically were more thermo-responsive than the individual oxysterols; (iv) the thermo-responsiveness of 7keto-containing vesicles was equivalent to that of 25 hydroxycholesterol (25OH)-containing vesicles; and (v) we have characterized the observed membrane dynamics. The results provide a new plausible mechanism: oxidative-stressed membranes in conjunction with temperature change induce membrane dynamics. These findings improve the mechanisms reported previously that attributed the induced dynamics solely to membrane oxidation.
authors: School of Materials Science, Japan Advanced Institute of Science and Technology (JAIST), 1-1 Asahidai, Nomi, Ishikawa 923-1292, Japan. munde@jaist.ac.jp
authors: Mun'delanji C
authors: MC
authors: Vestergaard
authors: Tsuyoshi
authors: T
authors: Yoda
authors: Tsutomu
authors: T
authors: Hamada
authors: Yoko
authors: Y
authors: Akazawa Ogawa
authors: Yasukazu
authors: Y
authors: Yoshida
authors: Masahiro
authors: M
authors: Takagi