title: Preparation of magnetic polymer material with phosphate group and its application to the enrichment of phosphopeptides.
abstract: As one of the most important post-translational modifications (PTM), reversible phosphorylation of protein is involved in many cellular processes. Enrichment and separation of phosphopeptides have become essential for large-scale identification of protein phosphorylation by mass spectrometry. In this work, five magnetic polymer materials with different numbers of phosphate groups were fabricated using a simple polymeric method and their abilities to enrich phosphopeptides were investigated. Our results showed that the enrichment efficiency is closely related to the number of phosphate groups attached to magnetic polymer sorbent. Under optimized condition (3% trifluoroacetic acid and 80% acetonitrile), magnetic polymer-particles with appropriate proportion of phosphate groups (Fe(3)O(4)@p(VPA-EDMA-1)-Zr(4+)) showed high performance for extracting phosphopeptides from complex peptides mixture of standard protein digestion. In this regard, a total of 988 unique phosphopeptides were successfully identified from proteolytic digestion of HeLa cell extracts by employing magnetic polymer-particles combined with nano-RPLC-MS/MS analysis.
authors: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan, China.
authors: Xiao-Shui
authors: XS
authors: Li
authors: Jian-Hong
authors: JH
authors: Wu
authors: Yong
authors: Y
authors: Zhao
authors: Wei-Ping
authors: WP
authors: Zhang
authors: Qiang
authors: Q
authors: Gao
authors: Lin
authors: L
authors: Guo
authors: Bi-Feng
authors: BF
authors: Yuan
authors: Yu-Qi
authors: YQ
authors: Feng