title: DNA typing of Pakistani cattle breeds Tharparkar and Red Sindhi by microsatellite markers.
abstract: Microsatellite markers are used for any individual identity and breed characterization in animals that is an efficient and successful way of investigation. They are used for multiple purposes as genetic detectors including, rapid mutation rate, high level of polymorphism, and range of variety of microsatellite markers available. A panel of 19 microsatellite markers was developed for breed characterization in Tharparkar and Red Sindhi breeds of cattle in Pakistan. Forty four blood samples of cattle (each breed) were collected from Department of Livestock Management, Sindh Agriculture University, Tandojam, Tando Qaiser, Tharparkar Cattle Farm Nabi sar Road, Umer Kot, Sindh, and Govt. Red Sindhi Cattle Breeding Farm, Tando Muhammad Khan Pakistan. Breed characterization was 100% successful. Average PIC, He and Power of Exclusion values were found to be 0.91, 0.62 and 13.28, respectively. Pattern of allelic frequencies of most of the microsatellite markers were clearly distinct between two breeds. As a result of present study a reliable, efficient and very informative panel of microsatellite markers was successfully developed which was capable to interpret individual identity, forensic cases and breed characterization in cattle. This facility is ready to be provided to local cattle breeder at commercial level for DNA testing of cattle. This study will also be highly helpful for breed conservation of cattle. In addition this study can also become a basis to open up new disciplines of animal forensics in Pakistan.
authors: Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore, Pakistan. ambazam@yahoo.com
authors: Amber
authors: A
authors: Azam
authors: Masroor Ellahi
authors: ME
authors: Babar
authors: Sehrish
authors: S
authors: Firyal
authors: Aftab Ahmad
authors: AA
authors: Anjum
authors: Nabeela
authors: N
authors: Akhtar
authors: Muhammad
authors: M
authors: Asif
authors: Tanveer
authors: T
authors: Hussain
references: Trop Anim Health Prod. 2006 Jan;38(1):65-74
references: 17405630
references: Genetika. 2009 Jul;45(7):941-8
references: 19705746
references: Am J Hum Genet. 1994 Jul;55(1):175-89
references: 7912887
references: Mol Biol Rep. 2011 Jan;38(1):411-6
references: 20339927
references: Mol Biol Rep. 2011 Feb;38(2):755-9
references: 20376703
references: Genetika. 2010 Jan;46(1):97-103
references: 20198885
references: BMC Genet. 2004 Jun 18;5:16
references: 15202952