title: No evidence for association between SLC11A1 and visceral leishmaniasis in India.
abstract: BACKGROUND
  SLC11A1 has pleiotropic effects on macrophage function and remains a strong candidate for infectious disease susceptibility. 5' and/or 3' polymorphisms have been associated with tuberculosis, leprosy, and visceral leishmaniasis (VL). Most studies undertaken to date were under-powered, and none has been replicated within a population. Association with tuberculosis has replicated variably across populations. Here we investigate SLC11A1 and VL in India.
  
  
  METHODS
  Nine polymorphisms (rs34448891, rs7573065, rs2276631, rs3731865, rs17221959, rs2279015, rs17235409, rs17235416, rs17229009) that tag linkage disequilibrium blocks across SLC11A1 were genotyped in primary family-based (313 cases; 176 families) and replication (941 cases; 992 controls) samples. Family- and population-based analyses were performed to look for association between SLC11A1 variants and VL. Quantitative RT/PCR was used to compare SLC11A1 expression in mRNA from paired splenic aspirates taken before and after treatment from 24 VL patients carrying different genotypes at the functional promoter GTn polymorphism (rs34448891).
  
  
  RESULTS
  No associations were observed between VL and polymorphisms at SLC11A1 that were either robust to correction for multiple testing or replicated across primary and replication samples. No differences in expression of SLC11A1 were observed when comparing pre- and post-treatment samples, or between individuals carrying different genotypes at the GTn repeat.
  
  
  CONCLUSIONS
  This is the first well-powered study of SLC11A1 as a candidate for VL, which we conclude does not have a major role in regulating VL susceptibility in India.
authors: Institute of Medical Sciences, Banaras Hindu University, Varanasi, OS 221 005, India.
authors: Sanjana
authors: S
authors: Mehrotra
authors: Joyce
authors: J
authors: Oommen
authors: Anshuman
authors: A
authors: Mishra
authors: Medhavi
authors: M
authors: Sudharshan
authors: Puja
authors: P
authors: Tiwary
authors: Sarra E
authors: SE
authors: Jamieson
authors: Michaela
authors: M
authors: Fakiola
authors: Deepa Selvi
authors: DS
authors: Rani
authors: Kumarasamy
authors: K
authors: Thangaraj
authors: Madhukar
authors: M
authors: Rai
authors: Shyam
authors: S
authors: Sundar
authors: Jenefer M
authors: JM
authors: Blackwell
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