title: Nocturnal hemodialysis is associated with restoration of early-outgrowth endothelial progenitor-like cell function.
abstract: OBJECTIVE
  Angiogenesis is a key response to tissue ischemia that may be impaired by uremia. Although early-outgrowth endothelial progenitor-like cells promote angiogenesis in the setting of normal renal function, cells from uremic patients are dysfunctional. When compared with conventional hemodialysis, it was hypothesized that nocturnal hemodialysis would improve the in vivo angiogenic activity of these cells in a well described model of ischemic vascular disease.
  
  
  METHODS
  Early-outgrowth endothelial progenitor-like cells were cultured from healthy controls (n = 5) and age- and gender-matched conventional hemodialysis (12 h/wk, n = 10) and nocturnal hemodialysis (30 to 50 h/wk, n = 9) patients. Cells (5 × 10(5)) or saline were injected into the ischemic hindlimb of athymic nude rats 1 day after left common iliac artery ligation.
  
  
  RESULTS
  Although conventional dialysis cell injection had no effect versus saline, nocturnal hemodialysis and healthy control cell injection significantly improved ischemic hindlimb perfusion and capillary density. Nocturnal hemodialysis cell injection was also associated with significant increases in endogenous angiopoietin 1 expression in the ischemic hindlimb compared with saline and conventional dialysis cell injection.
  
  
  CONCLUSIONS
  In contrast to a conventional dialytic regimen, nocturnal hemodialysis is associated with a significantly improved ability of early-outgrowth endothelial progenitor-like cells to promote angiogenesis and thus restore perfusion in a model of ischemic vascular disease.
authors: Keenan Research Centre of the Li Ka Shing Knowledge Institute, St. Michael's Hospital, Toronto, Ontario, Canada.
authors: Darren A
authors: DA
authors: Yuen
authors: Michael A
authors: MA
authors: Kuliszewski
authors: Christine
authors: C
authors: Liao
authors: Dmitriy
authors: D
authors: Rudenko
authors: Howard
authors: H
authors: Leong-Poi
authors: Christopher T
authors: CT
authors: Chan
references: Am J Kidney Dis. 1998 Nov;32(5 Suppl 3):S112-9
references: 9820470
references: N Engl J Med. 2003 Feb 13;348(7):593-600
references: 12584367
references: Nat Med. 2000 Apr;6(4):389-95
references: 10742145
references: Circ Res. 2001 Jul 6;89(1):E1-7
references: 11440984
references: Arterioscler Thromb Vasc Biol. 2008 Sep;28(9):1584-95
references: 18669889
references: Am J Kidney Dis. 2004 Nov;44(5):840-9
references: 15492950
references: Nephrol Dial Transplant. 2003 May;18(5):892-8
references: 12686661
references: Curr Opin Nephrol Hypertens. 2008 Nov;17(6):635-41
references: 19031658
references: J Am Coll Cardiol. 2008 Feb 12;51(6):660-8
references: 18261686
references: Kidney Int. 2002 Jun;61(6):2235-9
references: 12028465
references: JAMA. 2007 Sep 19;298(11):1291-9
references: 17878421
references: N Engl J Med. 2005 Jul 21;353(3):238-48
references: 16034009
references: N Engl J Med. 2009 Apr 2;360(14):1395-407
references: 19332456
references: J Am Soc Nephrol. 2001 Dec;12(12):2838-2847
references: 11729255
references: Science. 1997 Feb 14;275(5302):964-7
references: 9020076
references: PLoS One. 2010 Mar 04;5(3):e9543
references: 20209052
references: Am J Kidney Dis. 2003 Jan;41(1):225-9
references: 12500241
references: J Clin Invest. 2000 Jun;105(11):1527-36
references: 10841511
references: Circ Res. 2007 Aug 3;101(3):295-303
references: 17585071
references: Kidney Int. 2006 Oct;70(7):1318-24
references: 16871247
references: Hypertension. 2003 Nov;42(5):925-31
references: 14557284
references: Circ Res. 1998 Aug 10;83(3):233-40
references: 9710115
references: Arterioscler Thromb Vasc Biol. 2010 Oct;30(10):1897-904
references: 20689075
references: Blood. 2003 Aug 15;102(4):1340-6
references: 12702503
references: J Mol Cell Cardiol. 2006 Jul;41(1):86-96
references: 16780869
references: Nat Med. 2001 Apr;7(4):430-6
references: 11283669
references: J Mol Cell Cardiol. 2005 Nov;39(5):733-42
references: 16199052
references: Kidney Int. 2004 Aug;66(2):641-6
references: 15253717
references: ASAIO Trans. 1987 Jul-Sep;33(3):286-8
references: 3675955
references: J Am Soc Nephrol. 2003 May;14(5):1287-95
references: 12707397
references: Kidney Int. 1999 Nov;56(5):1928-33
references: 10571804
references: Proc Natl Acad Sci U S A. 2000 Mar 28;97(7):3422-7
references: 10725398
references: Am J Physiol Renal Physiol. 2005 Oct;289(4):F679-84
references: 15928211
references: J Am Soc Nephrol. 1998 Jun;9(6):1018-22
references: 9621284
references: Arterioscler Thromb Vasc Biol. 2004 Jul;24(7):1246-52
references: 15155385
references: Am J Respir Cell Mol Biol. 2010 Nov;43(5):546-54
references: 19995942
references: Lancet. 2002 Aug 10;360(9331):427-35
references: 12241713
references: N Engl J Med. 2006 Sep 21;355(12):1210-21
references: 16990384
references: N Engl J Med. 2006 Sep 21;355(12):1222-32
references: 16990385
references: Circulation. 2002 Jun 25;105(25):3017-24
references: 12081997
references: Nephrol Dial Transplant. 2006 May;21(5):1407-12
references: 16504981
references: Am J Kidney Dis. 2006 Oct;48(4):606-15
references: 16997057
references: Circulation. 2003 Mar 4;107(8):1164-9
references: 12615796
references: Circulation. 1998 Sep 29;98(13):1261-3
references: 9751672