title: Equilibration of (2)H labeling between body water and free amino acids: enabling studies of proteome synthesis.
abstract: Protein synthesis can be estimated by measuring the incorporation of a labeled amino acid into a proteolytic peptide. Although prelabeled amino acids are typically administered, recent studies have tested (2)H(2)O; the assumption is that there is rapid equilibration of (2)H (in body water) with the carbon-bound hydrogens of amino acids before those amino acids are incorporated into a protein(s). We have determined the temporal changes in (2)H labeling of body water and amino acids which should build confidence in (2)H(2)O-based studies of protein synthesis when one aims to measure the (2)H labeling of proteolytic peptides.
authors: Atherosclerosis, Merck Research Laboratories, Rahway, NJ 07065, USA.
authors: Kithsiri
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