title: Characterization of peptide chain length and constituency requirements for YejABEF-mediated uptake of microcin C analogues.
abstract: Microcin C (McC), a natural antibacterial compound consisting of a heptapeptide attached to a modified adenosine, is actively taken up by the YejABEF transporter, after which it is processed by cellular aminopeptidases, releasing the nonhydrolyzable aminoacyl adenylate, an inhibitor of aspartyl-tRNA synthetase. McC analogues with variable length of the peptide moiety were synthesized and evaluated in order to characterize the substrate preferences of the YejABEF transporter. It was shown that a minimal peptide chain length of 6 amino acids and the presence of an N-terminal formyl-methionyl-arginyl sequence are required for transport.
authors: Laboratory of Medicinal Chemistry, Rega Institute for Medical Research, Katholieke Universiteit Leuven, Minderbroedersstraat 10, B-3000 Leuven, Belgium.
authors: Gaston H M
authors: GH
authors: Vondenhoff
authors: Bart
authors: B
authors: Blanchaert
authors: Sophie
authors: S
authors: Geboers
authors: Teymur
authors: T
authors: Kazakov
authors: Kirill A
authors: KA
authors: Datsenko
authors: Barry L
authors: BL
authors: Wanner
authors: Jef
authors: J
authors: Rozenski
authors: Konstantin
authors: K
authors: Severinov
authors: Arthur
authors: A
authors: Van Aerschot
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