title: No mutations within conserved domain I of the p53 gene in medullary thyroid cancer.
abstract: Abnormal mobility shifts in single strand conformational polymorphism (SSCP)-analysis of exons 2 and 3 of the p53 gene in 2 out of 5 medullary thyroid cancers (MTC) prompted us to sequence the corresponding genomic region spanning codons 9-32. We found no mutations of the conserved domain I in the two MTCs that had previously shown mobility shifts, nor in a third MTC case indicating wild-type p53 (in this region). However, we detected a polymorphism consisting of a GC to CG transversion in intron 2 in two of the primary MTCs and one associated blood sample. In addition, we report on a deviation of the published sequence of intron 2 affecting positions 11817-8 and 11874, seen in 7/7 samples analyzed. Our findings corroborate the hypothesis, that MTC carcinogenesis does not require p53 mutations (Yana et al, Jpn J Cancer Res 1992).
authors: SUNY STONY BROOK,DEPT SURG,STONY BROOK,NY 11794. SUNY STONY BROOK,DEPT PATHOL,STONY BROOK,NY 11794. SUNY STONY BROOK,DEPT PHYSIOL & BIOPHYS,STONY BROOK,NY 11794. MICHIGAN CANC FDN,DETROIT,MI 48201.
authors: C
authors: C
authors: Lang
authors: M
authors: M
authors: Herrmann
authors: M
authors: M
authors: Elmaghrabi
authors: N
authors: N
authors: Abumrad
authors: M
authors: M
authors: Lane
authors: D
authors: D
authors: Baunoch
authors: U
authors: U
authors: Moll