title: [The expression and significance of Wnt/beta-catenin signal pathway protein in the effect of bushen huoxue granule containing serum on the osteoblast].
abstract: OBJECTIVE
  To investigate the effect of Wnt/beta-catenin signal pathway protein in the effect of Bushen Huoxue Granule (BHG) containing serum on the osteoblast, and to provide necessary experimental reliance for its action of mechanism in treatment of osteoporosis.
  
  
  METHODS
  The osteoblast from cranial bones of neonates rat were isolated and cultured in vitro, which was divided into the blank control group and the BHG containing serum group. The alkaline phosphatase (ALP) activities of the osteoblast in each group were quantitatively detected and the ALP staining was performed six days later. The alizarin red staining was performed eighteen days later. At the same time, levels of Wnt/beta-catenin signal pathway protein--beta-catenin, low density lipoprotein correlated protein 5 (LRP 5), and T cell factor (TCF) of osteoblasts in each group were detected by ELISA.
  
  
  RESULTS
  BHG containing serum could significantly increase the expression of ALP and promote the formation of mineralizing nodus in the osteoblasts. At the same time it also markedly up-regulated the expressions of p-catenin, LRP 5, and TCF in this process.
  
  
  CONCLUSIONS
  BHG containing serum could markedly increase ossify activities and mineralization of osteoblast. This action was closely correlated with Wnt/p-catenin signal pathway. So it indicated that Wnt/beta-catenin signal pathway played a very important role in the treatment of the osteoporosis by BHG.
authors: First Clinical Medical College, Zhejiang University of Traditional Chinese Medicine, Hangzhou.
authors: Ying-xing
authors: YX
authors: Xu
authors: Cheng-liang
authors: CL
authors: Wu
authors: Yan
authors: Y
authors: Wu