title: Newborn mice vaccination with BCG.HIVA²²² + MVA.HIVA enhances HIV-1-specific immune responses: influence of age and immunization routes.
abstract: We have evaluated the influence of age and immunization routes for induction of HIV-1- and M. tuberculosis-specific immune responses after neonatal (7 days old) and adult (7 weeks old) BALB/c mice immunization with BCG.HIVA(222) prime and MVA.HIVA boost. The specific HIV-1 cellular immune responses were analyzed in spleen cells. The body weight of the newborn mice was weekly recorded. The frequencies of HIV-specific CD8(+) T cells producing IFN-γ were higher in adult mice vaccinated intradermally and lower in adult and newborn mice vaccinated subcutaneously. In all cases the IFN-γ production was significantly higher when mice were primed with BCG.HIVA(222) compared with BCGwt. When the HIV-specific CTL activity was assessed, the frequencies of specific killing were higher in newborn mice than in adults. The prime-boost vaccination regimen which includes BCG.HIVA(222) and MVA.HIVA was safe when inoculated to newborn mice. The administration of BCG.HIVA(222) to newborn mice is safe and immunogenic and increased the HIV-specific responses induced by MVA.HIVA vaccine. It might be a good model for infant HIV and Tuberculosis bivalent vaccine.
authors: AIDS Research Unit, Hospital Clínic/IDIBAPS-HIVACAT, University of Barcelona, Calle Villarroel 170, 08036 Barcelona, Spain.
authors: Narcís
authors: N
authors: Saubi
authors: Eung-Jun
authors: EJ
authors: Im
authors: Raquel
authors: R
authors: Fernández-Lloris
authors: Olga
authors: O
authors: Gil
authors: Pere-Joan
authors: PJ
authors: Cardona
authors: Josep Maria
authors: JM
authors: Gatell
authors: Tomáš
authors: T
authors: Hanke
authors: Joan
authors: J
authors: Joseph
references: JAMA. 1994 Mar 2;271(9):698-702
references: 8309034
references: Adv Tuberc Res. 1984;21:107-93
references: 6475644
references: AIDS Res Hum Retroviruses. 1998 Dec 20;14(18):1625-33
references: 9870315
references: J Mol Evol. 2000 Jan;50(1):45-55
references: 10654259
references: Nature. 1991 Jun 6;351(6326):456-60
references: 1904554
references: Infect Immun. 2009 Aug;77(8):3364-73
references: 19487476
references: Infect Immun. 2003 Apr;71(4):1672-9
references: 12654780
references: J Immunol Methods. 2004 Nov;294(1-2):15-22
references: 15604012
references: Southeast Asian J Trop Med Public Health. 2009 Mar;40(2):273-81
references: 19323012
references: J Virol. 2007 Dec;81(23):12793-802
references: 17881444
references: J Virol. 2006 Feb;80(4):1645-52
references: 16439521
references: Cell. 2006 Feb 24;124(4):683-7
references: 16497578
references: J Virol. 2005 Oct;79(20):12871-9
references: 16188989
references: Nature. 1991 Jun 6;351(6326):479-82
references: 2046750
references: JAMA. 1995 Jan 18;273(3):220-6
references: 7807661
references: Vaccine. 2009 Dec 10;28(1):152-61
references: 19808028
references: J Virol. 2008 Oct;82(20):10199-206
references: 18667518
references: J Virol. 2007 Sep;81(17):9408-18
references: 17596303
references: Eur J Immunol. 1996 Jul;26(7):1489-96
references: 8766551
references: Vaccine. 2005 Jan 26;23(10):1209-24
references: 15652663
references: J Clin Invest. 2002 Jul;110(1):15-20
references: 12093882
references: Microbiology (Reading). 1996 Apr;142 ( Pt 4):915-925
references: 8936318
references: J Exp Med. 1993 Jul 1;178(1):197-209
references: 8315378
references: Proc Natl Acad Sci U S A. 1988 May;85(9):3105-9
references: 2452443
references: Proc Natl Acad Sci U S A. 1995 Nov 7;92(23):10693-7
references: 7479867
references: Microbes Infect. 2008 Feb;10(2):198-202
references: 18248757
references: Science. 1999 Feb 5;283(5403):857-60
references: 9933172
references: J Bacteriol. 1999 Aug;181(16):4780-9
references: 10438745
references: J Exp Med. 2004 Jun 21;199(12):1709-18
references: 15210746
references: J Immunol Methods. 2004 Feb 1;285(1):25-40
references: 14871532
references: Nat Med. 2000 Sep;6(9):951-5
references: 10973301
references: Expert Rev Vaccines. 2006 Dec;5(6):827-38
references: 17184220
references: J Immunol. 1999 Aug 15;163(4):2249-55
references: 10438968
references: J Immunol. 2002 Jan 15;168(2):919-25
references: 11777990
references: J Virol. 2010 Aug;84(15):7815-21
references: 20484495
references: J Biol Stand. 1988 Jan;16(1):15-26
references: 3350821
references: J Biomed Biotechnol. 2010;2010:357370
references: 20617151
references: Eur J Immunol. 2001 May;31(5):1531-5
references: 11465110
references: J Virol. 2009 Jun;83(11):5505-13
references: 19297477
references: J Immunol. 1993 Apr 1;150(7):3101-7
references: 8454878
references: Nat Med. 2004 Nov;10(11):1240-4
references: 15502839
references: J Virol. 1998 Feb;72(2):1497-503
references: 9445053
references: Vaccine. 2002 Dec 13;21(3-4):158-66
references: 12450689