title: Genotoxicity of endosseous implants using two cellular lineages in vitro.
abstract: The genotoxic potential of corrosion eluates obtained from a single dental implant using murine fibroblasts or osteoblasts cells in vitro by the single-cell gel (comet) assay was examined. A single commercially available dental implant (Biotechnology) was eluted in a solution consisting of equal amounts of acetic acid and sodium chloride (0.1 M) for 1, 3, 7, 14, and 21 days. Murine fibroblast or osteoblast cultures were then exposed to all corrosion eluates obtained from endosseous dental implants for 30 minutes at 37°C. The results suggest that none of the eluates produced genotoxic changes in murine fibroblasts regardless of the length of exposure to the eluate. Similarly, no genotoxicity was found in osteoblasts. The results suggest that the dental implant eluates tested in this study did not induce genetic damage as depicted by the single-cell gel (comet) assay. Because DNA damage is an important event during oncogenesis, this study represents a relevant contribution to estimate the real risks to the cellular system induced by the corrosion products of a dental implant.
authors.affiliation: 1  Department of Oral and Maxillofacial Surgery, University of Sacred Heart, USC, SP, Brazil.
authors.forename: Mariza
authors.initials: M
authors.lastname: Matsumoto
authors.forename: Hugo Nary
authors.initials: HN
authors.lastname: Filho
authors.forename: Raquel
authors.initials: R
authors.lastname: Ferrari
authors.forename: Kristianne
authors.initials: K
authors.lastname: Fernandes
authors.forename: Ana Claudia
authors.initials: AC
authors.lastname: Renno
authors.forename: Daniel
authors.initials: D
authors.lastname: Ribeiro
mesh_terms: D016475:3T3 Cells; D019342:Acetic Acid / Q000737:chemistry; D000818:Animals; D002460:Cell Line; D020552:Comet Assay; D003343:Corrosion; D004249:DNA Damage; D015921:Dental Implants; D003764:Dental Materials / Q000737:chemistry / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects*; D051379:Mice; D009153:Mutagens / Q000494:pharmacology*; D010006:Osteoblasts / Q000187:drug effects*; D051381:Rats; D012965:Sodium Chloride / Q000737:chemistry; D013696:Temperature; D013997:Time Factors; D014025:Titanium / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D015921:Dental Implants; D003764:Dental Materials; D009153:Mutagens; D012965:Sodium Chloride; D014025:Titanium; D019342:Acetic Acid