title: Escherichia coli K5 heparosan fermentation and improvement by genetic engineering.
abstract: N-acetyl heparosan is the precursor for the biosynthesis of the important anticoagulant drug heparin. The E. coli K5 capsular heparosan polysaccharide provides a promising precursor for in vitro chemoenzymatic production of bioengineered heparin. This article explores the improvements of heparosan production for bioengineered heparin by fermentation process engineering and genetic engineering.
authors.affiliation: Department of Biology, Center for Biotechnology & Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, USA.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Wang
authors.forename: Jonathan S
authors.initials: JS
authors.lastname: Dordick
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Linhardt
mesh_terms: D016667:Bacterial Capsules / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005285:Fermentation; D005818:Genetic Engineering / Q000379:methods*; D006493:Heparin / Q000235:genetics / Q000378:metabolism; D008954:Models, Biological; D015394:Molecular Structure
chemical_list: C050673:capsular polysaccharide K5; D006493:Heparin