title: Identification of the piroplasms isolated from horses with clinical piroplasmosis in Poland.
abstract: The study was aimed at determining the cause of the diseases in three horses exhibiting symptoms of fever, ataxia, mucus membrane paleness, haematuria and thrombocytopenia. The PCR technique revealed the presence in the blood of 18S RNA Babesia/Theileria spp. genetic material. DNA amplification using primers RLB F2 and RLB R2 produced 430 bp size products. The sequences of these PCR products demonstrated a 95.6-97.5% similarity with the sequence of the fragment of 18S RNA Babesia equi, gene number DQ287951 in the GenBank. The treatment utilizing the subcutaneous application of the imidocarb resulted in gradual recovery of the diseased animals.
authors.affiliation: Department of Epizootiology and Clinic of Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences, 30 Gleboka Street, 20-612 Lublin, Poland. ukaszek0@wp.pl
authors.forename: Łukasz
authors.initials: Ł
authors.lastname: Adaszek
authors.forename: Marta
authors.initials: M
authors.lastname: Górna
authors.forename: Michał
authors.initials: M
authors.lastname: Krzysiak
authors.forename: Michał
authors.initials: M
authors.lastname: Adaszek
authors.forename: Marcin
authors.initials: M
authors.lastname: Garbal
authors.forename: Stanisław
authors.initials: S
authors.lastname: Winiarczyk
mesh_terms: D000818:Animals; D001404:Babesiosis / Q000175:diagnosis* / Q000469:parasitology / Q000662:veterinary*; D006734:Horse Diseases / Q000175:diagnosis* / Q000469:parasitology*; D006736:Horses; D016791:Piroplasmia / Q000235:genetics / Q000302:isolation & purification*; D011044:Poland; D011529:Protozoan Infections, Animal / Q000175:diagnosis* / Q000469:parasitology*; D016053:RNA, Protozoan / Q000302:isolation & purification
chemical_list: D016053:RNA, Protozoan