title: AC-NP: a novel chimeric peptide with natriuretic and vasorelaxing actions.
abstract: The aim of this study was to evaluate the cardiovascular and renal activities of a newly designed natriuretic peptide (NP). Here, we engineered a novel 28-amino acid chimeric peptide, termed AC-NP that combined the 17-amino acid ring of C type natriuretic peptide (CNP) with the 6-amino acid N-terminus and 5-amino acid C-terminus of atrial natriuretic peptide (ANP). Both in vitro and in vivo experiments were performed to determine the actions of AC-NP. In normal rats, AC-NP proved to be more potentially diuretic, natriuretic and hypotensive compared with other NPs, such as ANP, CNP and vasonatrin peptide (VNP), which is another man-made NP. In relaxation of isolated abdominal aorta from rat, AC-NP was equally effective to ANP, CNP and VNP. Elevated levels of 3',5'-guanosine monophosphate (cGMP) in plasma and urine cGMP excretion indicated the participation of cGMP in the functions of AC-NP. Taken together, innovative designed AD-NP might be a new candidate therapeutic peptide against cardiorenal disorders.
authors.affiliation: Department of Radiology, Tangdu Hospital, Fourth Military Medical University, Xi'an, China.
authors.forename: Bao-Ying
authors.initials: BY
authors.lastname: Chen
authors.forename: Jian-Kang
authors.initials: JK
authors.lastname: Chen
authors.forename: Miao-Zhang
authors.initials: MZ
authors.lastname: Zhu
authors.forename: Dong-Liang
authors.initials: DL
authors.lastname: Zhang
authors.forename: Jie-Shen
authors.initials: JS
authors.lastname: Sun
authors.forename: Jian-Ming
authors.initials: JM
authors.lastname: Pei
authors.forename: Hua-Song
authors.initials: HS
authors.lastname: Feng
authors.forename: Xiao-Xing
authors.initials: XX
authors.lastname: Zhu
authors.forename: Jian
authors.initials: J
authors.lastname: Jin
authors.forename: Jun
authors.initials: J
authors.lastname: Yu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001012:Aorta, Abdominal / Q000187:drug effects / Q000502:physiology; D009320:Atrial Natriuretic Factor / Q000737:chemistry / Q000494:pharmacology*; D006321:Heart / Q000187:drug effects / Q000502:physiology; D006801:Humans; D066298:In Vitro Techniques; D007668:Kidney / Q000187:drug effects / Q000502:physiology; D008297:Male; D008969:Molecular Sequence Data; D009318:Natriuresis / Q000187:drug effects*; D020098:Natriuretic Peptide, C-Type / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000494:pharmacology*; D014664:Vasodilation / Q000187:drug effects*
chemical_list: D011993:Recombinant Fusion Proteins; D020098:Natriuretic Peptide, C-Type; D009320:Atrial Natriuretic Factor