title: [MicroRNA-144 over-expression induced myocytes apoptosis].
abstract: OBJECTIVE
  To investigate the effects of microRNA-144 (miR-144) expression on H9C2 (2-1) myocytes.
  
  
  METHODS
  MiR-144 was up-regulated in primary cultured H9C2 (2-1) myocytes through transfection. Cells transfected with Lipofectamine(TM) 2000 and its mixture with miRNA synthesized randomly as blank control and negative control respectively. The up-regulation of miR-144 was confirmed by real-time PCR. Cell apoptosis was evaluated by means of CCK-8, Caspase-3 and flow cytometry.
  
  
  RESULTS
  Real-time PCR results showed that the miR-144 expression was obviously increased in miR-144 up-regulation group (2178.84 ± 838.52) compared with negative (2.06 ± 0.73) and blank (1.00 ± 0.00) control group (all P < 0.01). The proliferation was lower, the activity of Caspase-3 was elevated and the apoptosis rates were significantly increased in miR-144 up-regulation group compared with negative and blank control group, while no significant difference was found between the latter 2 groups.
  
  
  CONCLUSIONS
  MiR-144 mimics may selectively up-regulate the expression of miR-144 in myocardial cells and consequently promote apoptosis and inhibit proliferation in myocardial cells.
authors.affiliation: Department of Cardiology, First Affiliated Hospital, Institute for Cardiovascular Biology & Gene, Wenzhou Medical College, Wenzhou 325000, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Huang
authors.forename: Xiao-yan
authors.initials: XY
authors.lastname: Huang
authors.forename: Dong-sheng
authors.initials: DS
authors.lastname: Yan
authors.forename: Xi
authors.initials: X
authors.lastname: Zhou
authors.forename: De-ye
authors.initials: DY
authors.lastname: Yang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D053148:Caspase 3 / Q000378:metabolism; D002460:Cell Line; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D032342:Muscle Cells / Q000378:metabolism*; D051381:Rats; D012844:Sincalide / Q000378:metabolism; D014162:Transfection
chemical_list: C555034:MIRN144 microRNA, rat; D035683:MicroRNAs; C505410:Casp3 protein, rat; D053148:Caspase 3; D012844:Sincalide