title: Detection of bacteria using bacteriophages as recognition elements immobilized on long-period fiber gratings.
abstract: The paper presents for the first time a study of long-period gratings (LPGs) applied for label-free detection of specific bacteria using physically adsorbed bacteriophages. For the purposes of the experiment a number of highly sensitive LPGs working at the turning point of phase matching curve was fabricated in SMF28 fiber using UV exposure. We show that the device allows for real-time monitoring of phenomena taking place on the sensor's surface, including phage-bacteria interactions. For the applied conditions a resonance wavelength shift of ~1.3 nm induced by bacteria binding was observed.
authors.affiliation: Centre de recherche en photonique, Université du Québec en Outaouais, Gatineau, QC, Canada. m.smietana@elka.pw.edu.pl
authors.forename: Mateusz
authors.initials: M
authors.lastname: Smietana
authors.forename: Wojtek J
authors.initials: WJ
authors.lastname: Bock
authors.forename: Predrag
authors.initials: P
authors.lastname: Mikulic
authors.forename: Andy
authors.initials: A
authors.lastname: Ng
authors.forename: Raja
authors.initials: R
authors.lastname: Chinnappan
authors.forename: Mohammed
authors.initials: M
authors.lastname: Zourob
mesh_terms: D001422:Bacterial Adhesion / Q000502:physiology; D058491:Bacterial Load / Q000295:instrumentation*; D017122:Bacteriophage T4 / Q000502:physiology*; D001681:Biological Assay / Q000295:instrumentation*; D015374:Biosensing Techniques / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D004926:Escherichia coli / Q000302:isolation & purification* / Q000502:physiology*; D012031:Refractometry / Q000295:instrumentation*