title: Electrospun Poly(γ-Benzyl-L-Glutamate) and Its Alkali-Treated Meshes: Their Water Wettability and Cell-Adhesion Potential.
abstract: The aim of this study was (1) to fabricate non-woven meshes from a biodegradable polymer, poly(γ-benzylL-glutamate), by electrospinning and subsequent hydrolysis of the ester bond on the polymer side-chain in an aqueous solution of NaOH, creating surface carboxyl groups on the fibers, and (2) to determine the effect of hydrolysis time on water wettability and cellular behaviors, in order to perform a preliminary evaluation for use of this polymer as a wound dressing matrix. A non-woven mesh composed of fibers, with minimal formation of 'bead' structures, was produced by electrospinning from tetrahydrofuran solution under optimally controlled conditions. The surface wettability largely depended on the hydrolysis time: an increase in hydrolysis time significantly reduced the advancing water contact angle. Instantaneous spreading of water droplets occurred at long hydrolysis times. An increase in hydrolysis time decreased adhesion of endothelial cells, but increased cell spreading. Neither proliferation nor invasion into the mesh interior was observed. We conclude by discussing the use of partially hydrolyzed non-woven mesh as a promising burn dressing.
authors.affiliation: a Genome Biotechnology Laboratories, Kanazawa Institute of Technology, 3-1 Yasukaho, Hakusan, Ishikawa 924-0838, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Hakamada
authors.forename: Nao
authors.initials: N
authors.lastname: Ohgushi
authors.forename: Nozomi
authors.initials: N
authors.lastname: Fujimura-Kondo
authors.forename: Takehisa
authors.initials: T
authors.lastname: Matsuda
mesh_terms: D001593:Benzyl Compounds / Q000737:chemistry* / Q000494:pharmacology*; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D004560:Electricity; D005971:Glutamates / Q000737:chemistry* / Q000494:pharmacology*; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000187:drug effects; D006801:Humans; D006868:Hydrolysis; D012972:Sodium Hydroxide / Q000737:chemistry; D014867:Water / Q000737:chemistry*; D017155:Wettability; D014945:Wound Healing / Q000187:drug effects
chemical_list: D001593:Benzyl Compounds; D001672:Biocompatible Materials; D005971:Glutamates; C582972:poly-(gamma-benzyl-L-glutamate); D014867:Water; D012972:Sodium Hydroxide