title: Direct On-Line Coupling of Capillary HPLC with (1)H NMR Spectroscopy for the Structural Determination of Retinyl Acetate Dimers:  2D NMR Spectroscopy in the Nanoliter Scale.
abstract: This paper presents the application of directly coupled capillary high-performance liquid chromatography (capillary HPLC) and proton high-field nuclear magnetic resonance spectroscopy (NMR) for structural elucidation of a so-far unknown kitol isomer. One- and two-dimensional continuous- and stopped-flow NMR spectra were recorded in a 180 μm i.d. capillary, corresponding to a detection volume of only 200 nL. Unequivocal structural assignment on the basis of 1D and 2D stopped-flow capillary HPLC-NMR experiments was performed. The kitol isomer mixture was present in a sample of thermally isomerized retinyl acetate and separated on a capillary column.
authors.affiliation: Institut für Organische Chemie, Universität Tübingen, Auf der Morgenstelle 18, D-72076 Tübingen, Germany, and Bruker Analytische Messtechnik GmbH, Silberstreifen, D-76287 Rheinstetten, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Schlotterbeck
authors.forename: L H
authors.initials: LH
authors.lastname: Tseng
authors.forename: H
authors.initials: H
authors.lastname: Händel
authors.forename: U
authors.initials: U
authors.lastname: Braumann
authors.forename: K
authors.initials: K
authors.lastname: Albert