title: Sasa quelpaertensis phenylpropanoid derivative suppresses lipopolysaccharide-induced nitric oxide synthase and cyclo-oxygenase-2 expressions in RAW 264.7 cells.
abstract: 3-O-p-Coumaroyl-1-(4-hydroxy-3,5-dimethoxyphenyl)-1-O-β-D-gulcopyranosylpropanol (ESQ10) is a naturally occurring phenylpropanoid derivative isolated from Sasa quelpaertensis (Gramineae). In the present study, we discovered that ESQ10 inhibits nitric oxide (NO) and prostaglandin E(2) (PGE(2)) production in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. ESQ10 attenuated LPS-induced synthesis of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in parallel and inhibited LPS-induced interleukin-6 production, as determined by an enzyme-linked immunosorbent assay in the macrophages. The mechanism of the antiinflammatory action of ESQ10, i.e., suppression of nuclear factor (NF)-κB and mitogen-activated protein kinase activation, has been documented. However, ESQ10 could not influence LPS-mediated IκB-α degradation and extracellular signal-regulated kinase/c-Jun amino-terminal kinase phosphorylation at concentrations of up to 373 µM. To test the potential application of ESQ10 as a topical material, we also conducted a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay on human HaCaT keratinocytes as well as human dermal fibroblast cells. In this assay, ESQ10 did not induce cytotoxicity. Taken together, the results suggest that ESQ10 may be considered an antiinflammatory candidate for treating inflammatory and skin diseases.
authors.affiliation: Bio-Convergence Center, Jeju Technopark, Jeju, Korea.
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Moon
authors.forename: Eun-Jin
authors.initials: EJ
authors.lastname: Yang
authors.forename: Sang Suk
authors.initials: SS
authors.lastname: Kim
authors.forename: Ji-Yong
authors.initials: JY
authors.lastname: Kang
authors.forename: Gi-Ok
authors.initials: GO
authors.lastname: Kim
authors.forename: Nam Ho
authors.initials: NH
authors.lastname: Lee
authors.forename: Chang-Gu
authors.initials: CG
authors.lastname: Hyun
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D003373:Coumaric Acids / Q000302:isolation & purification / Q000494:pharmacology*; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D016207:Cytokines / Q000378:metabolism; D003864:Depression, Chemical; D005960:Glucosides / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000201:enzymology*; D051379:Mice; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism*; D031787:Sasa / Q000737:chemistry*
chemical_list: C569005:3-O-p-coumaroyl-1-(4-hydroxy-3,5-dimethoxyphenyl)-1-O-glucopyranosylpropanol; D000893:Anti-Inflammatory Agents; D003373:Coumaric Acids; D016207:Cytokines; D005960:Glucosides; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D052247:Nitric Oxide Synthase Type II; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2