title: Ameliorative effect of caffeic acid against inflammatory pain in rodents.
abstract: The present study was designed to evaluate the antinociceptive profile of caffeic acid in mice and rats. Caffeic acid (5-100 mg/kg, p.o.), in a dose dependent manner inhibited acetic acid-induced writhing and late phase of formalin-induced pain in mice, with an ED(50) of 22.38 and 10.92 mg/kg, respectively. However, caffeic acid was ineffective in the hot plate and tail flick tests. Analgesic activity was also examined in carrageenan and lipopolysaccharide (LPS)-induced mechanical hyperalgesia in rats, where locally induced myeloperoxidase (MPO), malondialdehyde (MDA) and nitrite levels in foot pad were estimated by colorimetric assay. Oral administration of caffeic acid (200mg/kg, p.o.) showed analgesic activity similar to nimesulide (4 mg/kg, p.o.) and inhibited MPO, MDA and nitrite generation in the inflamed paw. Histological examination revealed reduction in neutrophil infiltration and protection of tissue damage by caffeic acid. These results suggest that caffeic acid exhibits peripheral analgesic effect in mice and rats and could be further examined for the treatment of chronic painful episodes.
authors.affiliation: Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal-576 104, Karnataka, India.
authors.forename: Anurag
authors.initials: A
authors.lastname: Mehrotra
authors.forename: Ravindranath
authors.initials: R
authors.lastname: Shanbhag
authors.forename: Mallikarjuna Rao
authors.initials: MR
authors.lastname: Chamallamudi
authors.forename: Vijay Pal
authors.initials: VP
authors.lastname: Singh
authors.forename: Jayesh
authors.initials: J
authors.lastname: Mudgal
mesh_terms: D000085:Acetates / Q000009:adverse effects; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D002109:Caffeic Acids / Q000494:pharmacology* / Q000627:therapeutic use; D002351:Carrageenan / Q000009:adverse effects; D005557:Formaldehyde / Q000009:adverse effects; D007249:Inflammation / Q000139:chemically induced / Q000150:complications; D008070:Lipopolysaccharides / Q000009:adverse effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D009573:Nitrites / Q000378:metabolism; D010146:Pain / Q000150:complications* / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D009195:Peroxidase / Q000378:metabolism; D051381:Rats
chemical_list: D000085:Acetates; D002109:Caffeic Acids; D008070:Lipopolysaccharides; D009573:Nitrites; D005557:Formaldehyde; D008315:Malondialdehyde; D002351:Carrageenan; D009195:Peroxidase; C040048:caffeic acid