title: Metabonomics study of brain-specific human S100B transgenic mice by using high-performance liquid chromatography coupled with quadrupole time of flight mass spectrometry.
abstract: For the purpose of investigating the effects of S100B on the development of Parkinsion's disease (PD), a high-performance liquid chromatography coupled with electrospray ionization time-of-flight mass spectrometry (HPLC/MS-ESI-TOF) metabonomic approach was established to study the mesencephalon profiling of brain-specific human S100B transgenic mice. In order to obtain more full-scale chemical information of metabolites, two kinds of separation mechanism, including reversed-phase (RP) column chromatography and hydrophilic interaction liquid chromatography (HILIC) column, were combined to use. Acquired data were subjected to principal component analysis (PCA) to investigate the effects of S100B protein on mice mesencephalon metabolite profiles. Potential biomarkers were screened by using Mass Hunter Prossional Profiller (MPP) and were identified by the accurate mass. Twelve metabolites in mesencephalon of S100B transgenic mice were identified as potential biomarkers, among which, glutamic acid (Glu) detected by RP/MS in negative ionization mode, gamma-aminobutyric acid (GABA) and tryptophan (Trp) detected by HILIC/MS in positive ionization mode, phenylalanine (Phe) and histidine (His) detected by HILIC/MS in negative ionization mode, related to metabolic pathway of neurotransmitters in mice central nervous system. The analytical technique used in this paper was able to detect biochemical changes in mesencephalon of S100B transgenic mice, which may be helpful to understand the action mechanism of S100B protein in the development of PD.
authors.affiliation: Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Peking Union Medical College, Key Laboratory of Human Disease Animal Model, State Administration of Traditional Chinese Medicine, Beijing, P. R. China.
authors.forename: Jia Lin
authors.initials: JL
authors.lastname: Liu
authors.forename: Hai Lin
authors.initials: HL
authors.lastname: Wang
authors.forename: Lian Feng
authors.initials: LF
authors.lastname: Zhang
authors.forename: Yan Feng
authors.initials: YF
authors.lastname: Xu
authors.forename: Wei
authors.initials: W
authors.lastname: Deng
authors.forename: Hua
authors.initials: H
authors.lastname: Zhu
authors.forename: Chuan
authors.initials: C
authors.lastname: Qin
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004195:Disease Models, Animal; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008297:Male; D008636:Mesencephalon / Q000378:metabolism*; D055432:Metabolomics / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009414:Nerve Growth Factors / Q000096:biosynthesis* / Q000235:genetics; D009474:Neurons / Q000378:metabolism*; D010300:Parkinson Disease / Q000378:metabolism* / Q000503:physiopathology; D010982:Platelet-Derived Growth Factor / Q000235:genetics; D025341:Principal Component Analysis; D011401:Promoter Regions, Genetic; D015203:Reproducibility of Results; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins / Q000096:biosynthesis* / Q000235:genetics; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000596:Amino Acids; D015415:Biomarkers; D009414:Nerve Growth Factors; D010982:Platelet-Derived Growth Factor; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins; C579160:S100B protein, human; C579159:S100b protein, mouse