title: Field testing of collection cards for Cannabis sativa samples with a single hexanucleotide DNA marker.
abstract: The validity and feasibility of using DNA collection cards in the field for preservation and analysis of Cannabis sativa genotypes were investigated using a highly specific hexanucleotide marker. Collection cards were submitted to the National Marijuana Initiative, which selectively trained and managed the collection of specific types of samples from a variety of participating agencies. Samples collected at seizure sites included fresh marijuana leaf samples, dried "dispensary" samples, U.S. border seizures, and hashish. Using a standardized PCR kit with custom-labeled oligonucleotide primers specific to marijuana, collection cards produced eight genotypes and 13 different alleles, extremely low baselines, and no cross-reactivity with control plant species. Results were produced from all sample types with the exception of hashish. Plant DNA collection cards represent an easily implementable method for the genetic identification and relatedness of C. sativa street and grow site-seized samples with applications for databasing and market disruption.
authors.affiliation: Forensic Science Department, Henry C. Lee College of Criminal Justice & Forensic Sciences, University of New Haven, 300 Boston Post Road, West Haven, CT 06516, USA.
authors.forename: Lindsay
authors.initials: L
authors.lastname: Allgeier
authors.forename: John
authors.initials: J
authors.lastname: Hemenway
authors.forename: Nicholas
authors.initials: N
authors.lastname: Shirley
authors.forename: Tommy
authors.initials: T
authors.lastname: LaNier
authors.forename: Heather Miller
authors.initials: HM
authors.lastname: Coyle
mesh_terms: D000483:Alleles; D002188:Cannabis / Q000235:genetics*; D017931:DNA Primers; D018744:DNA, Plant / Q000235:genetics*; D004335:Drug and Narcotic Control; D005240:Feasibility Studies; D005819:Genetic Markers; D005838:Genotype; D006801:Humans; D035846:Law Enforcement; D018515:Plant Leaves; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers