title: The complete chloroplast genome of tall fescue (Lolium arundinaceum; Poaceae) and comparison of whole plastomes from the family Poaceae.
abstract: In this paper, we describe the complete chloroplast genome of Lolium arundinaceum. This sequence is the culmination of a long-term project completed by >400 undergraduates who took general genetics at Middle Tennessee State University from 2004-2007. It was undertaken in an attempt to introduce these students to an open-ended experiential/exploratory lesson to produce and analyze novel data. The data they produced should provide the necessary information for both phylogenetic comparisons and plastome engineering of tall fescue. The fescue plastome (GenBank FJ466687) is 136048 bp with a typical quadripartite structure and a gene order similar to other grasses; 56% of the plastome is coding region comprised of 75 protein-coding genes, 29 tRNAs, four rRNAs, and one hypothetical coding region (ycf). Comparisons of Poaceae plastomes reveal size differences between the PACC (subfamilies Panicoideae, Arundinoideae, Centothecoideae, and Chloridoideae) and BOP (subfamilies Bambusoideae, Oryzoideae, and Pooideae) clades. Alignment analysis suggests that several potentially conserved large deletions in previously identified intergenic length polymorphic regions are responsible for the majority of the size discrepancy. Phylogenetic analysis using whole plastome data suggests that fescue closely aligns with Lolium perenne. Some unique features as well as phylogenetic branch length calculations, however, suggest that a number of changes have occurred since these species diverged.
authors.affiliation: Department of Biology, Middle Tennessee State University, Box 60, Murfreesboro, Tennessee 37132 USA.
authors.forename: A Bruce
authors.initials: AB
authors.lastname: Cahoon
authors.forename: Richard M
authors.initials: RM
authors.lastname: Sharpe
authors.forename: Chansamone
authors.initials: C
authors.lastname: Mysayphonh
authors.forename: Elizabeth J
authors.initials: EJ
authors.lastname: Thompson
authors.forename: Andrea D
authors.initials: AD
authors.lastname: Ward
authors.forename: Anhua
authors.initials: A
authors.lastname: Lin