title: OsJAR1 and OsJAR2 are jasmonyl-L-isoleucine synthases involved in wound- and pathogen-induced jasmonic acid signalling.
abstract: The synthesis of JA-Ile was catalysed by JA-Ile synthase, which is a member of the group I GH3 family of proteins. Here, we showed evidence that OsGH3.5 (OsJAR1) and OsGH3.3 (OsJAR2) are the functional JA-Ile synthases in rice, using recombinant proteins. The expression levels of OsJAR1 and OsJAR2 were induced in response to wounding with the concomitant accumulation of JA-Ile. In contrast, only the expression of OsJAR1 was associated with the accumulation of JA-Ile after blast infection. Our data suggest that these two JA-Ile synthases are differentially involved in the activation of JA signalling in response to wounding and pathogen challenge in rice.
authors.affiliation: Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Wakuta
authors.forename: Erika
authors.initials: E
authors.lastname: Suzuki
authors.forename: Wataru
authors.initials: W
authors.lastname: Saburi
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Matsuura
authors.forename: Kensuke
authors.initials: K
authors.lastname: Nabeta
authors.forename: Ryozo
authors.initials: R
authors.lastname: Imai
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Matsui
mesh_terms: D003517:Cyclopentanes / Q000378:metabolism*; D007532:Isoleucine / Q000031:analogs & derivatives* / Q000096:biosynthesis; D012275:Oryza / Q000201:enzymology* / Q000382:microbiology; D054883:Oxylipins / Q000378:metabolism*; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology*; D010940:Plant Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D003517:Cyclopentanes; D054883:Oxylipins; D010940:Plant Proteins; C532883:jasmonoyl-isoleucine; D007532:Isoleucine; C011006:jasmonic acid