title: Expression of the inflammatory chemokines CCL2, CCL5 and CXCL2 and the receptors CCR1-3 and CXCR2 in T lymphocytes from mammary tumor-bearing mice.
abstract: Chemokines and their receptors have been studied in several solid tumor models as mediators of inflammation. In turn, inflammation has been implicated in the promotion and progression of tumors, and as such, chemokines have been proposed as novel molecular targets for chemotherapy. While the expression of these molecules has been described in tumor cells, endothelial cells, macrophages and neutrophils, less attention has been paid to the expression profile of these molecules by T lymphocytes in the periphery or infiltrating the tumor. Using the D1-DMBA-3 murine mammary adenocarcinoma model, we aimed to better characterize the differential expression of chemokines and/or their receptors in the host and in the tumor microenvironment, and specifically, in the T cells of tumor-bearing mice compared to normal control animals. We found that T lymphocytes from tumor-bearing mice express the pro-inflammatory chemokines, CCL2, CCL5 and CXCL2, as well as the chemokine receptors, CCR1, CCR2, CCR3 and CXCR2.
authors.affiliation: Department of Physiological Sciences, University of Florida College of Veterinary Medicine, Gainesville, FL 32610, USA.
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Owen
authors.forename: Michael F
authors.initials: MF
authors.lastname: Criscitiello
authors.forename: Stephania
authors.initials: S
authors.lastname: Libreros
authors.forename: Ramon
authors.initials: R
authors.lastname: Garcia-Areas
authors.forename: Kathleen
authors.initials: K
authors.lastname: Guthrie
authors.forename: Marta
authors.initials: M
authors.lastname: Torroella-Kouri
authors.forename: Vijaya
authors.initials: V
authors.lastname: Iragavarapu-Charyulu
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000276:immunology; D000818:Animals; D045744:Cell Line, Tumor; D018932:Chemokine CCL2 / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D018946:Chemokine CCL5 / Q000235:genetics / Q000378:metabolism; D054426:Chemokine CXCL2 / Q000235:genetics / Q000378:metabolism; D018925:Chemokines / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D018836:Inflammation Mediators / Q000378:metabolism*; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology; D008325:Mammary Neoplasms, Experimental / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics; D054389:Receptors, CCR1 / Q000235:genetics / Q000378:metabolism; D054390:Receptors, CCR2 / Q000235:genetics / Q000378:metabolism; D054397:Receptors, CCR3 / Q000235:genetics / Q000378:metabolism; D019707:Receptors, Chemokine / Q000235:genetics / Q000378:metabolism*; D023063:Receptors, Interleukin-8B / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D016176:T-Lymphocyte Subsets / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: C491768:Ccl2 protein, mouse; C503460:Ccl5 protein, mouse; C515301:Ccr1 protein, mouse; C515335:Ccr2 protein, mouse; C515351:Ccr3 protein, mouse; D018932:Chemokine CCL2; D018946:Chemokine CCL5; D054426:Chemokine CXCL2; D018925:Chemokines; C404915:Cxcl2 protein, mouse; D018836:Inflammation Mediators; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D054389:Receptors, CCR1; D054390:Receptors, CCR2; D054397:Receptors, CCR3; D019707:Receptors, Chemokine; D023063:Receptors, Interleukin-8B; D011994:Recombinant Proteins