title: Yeast-based protein delivery to mammalian phagocytic cells is increased by coexpression of bacterial listeriolysin.
abstract: Yeast-mediated protein delivery to mammalian antigen-presenting cells is a powerful approach for inducing cell-mediated immune responses. We show that coexpression of the pore-forming protein listeriolysin O from Listeria monocytogenes leads to improved translocation of a proteinaceous antigen and subsequent activation of specific T lymphocytes. As the resulting yeast carrier is self-attenuated and killed after antigen delivery without exhibiting any toxic effect on antigen-presenting cells, this novel carrier system suggests itself as promising approach for the development of yeast-based live vaccines.
authors.affiliation: Molecular and Cell Biology, Building A1.5, Saarland University, 66123 Saarbrücken, Germany.
authors.forename: Barbara
authors.initials: B
authors.lastname: Walch
authors.forename: Tanja
authors.initials: T
authors.lastname: Breinig
authors.forename: Gernot
authors.initials: G
authors.lastname: Geginat
authors.forename: Manfred J
authors.initials: MJ
authors.lastname: Schmitt
authors.forename: Frank
authors.initials: F
authors.lastname: Breinig
mesh_terms: D017951:Antigen Presentation; D000938:Antigen-Presenting Cells / Q000378:metabolism / Q000382:microbiology*; D000941:Antigens / Q000276:immunology / Q000378:metabolism*; D001427:Bacterial Toxins / Q000096:biosynthesis* / Q000235:genetics; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D006460:Hemolysin Proteins / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D008565:Membrane Proteins / Q000276:immunology / Q000378:metabolism*; D010586:Phagocytes / Q000378:metabolism / Q000382:microbiology*; D015003:Yeasts / Q000235:genetics / Q000378:metabolism*
chemical_list: D000941:Antigens; D001427:Bacterial Toxins; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; D008565:Membrane Proteins; C038705:hlyA protein, Listeria monocytogenes