title: An XMRV derived retroviral vector as a tool for gene transfer.
abstract: BACKGROUND
  Retroviral vectors are widely used tools for gene delivery and gene therapy. They are useful for gene expression studies and genetic manipulation in vitro and in vivo. Many retroviral vectors are derived from the mouse gammaretrovirus, murine leukemia virus (MLV). These vectors have been widely used in gene therapy clinical trials. XMRV, initially found in prostate cancer tissue, was the first human gammaretrovirus described.
  
  
  RESULTS
  We developed a new retroviral vector based on XMRV called pXC. It was developed for gene transfer to human cells and is produced by transient cotransfection of LNCaP cells with pXC and XMRV-packaging plasmids.
  
  
  CONCLUSIONS
  We demonstrated that pXC mediates expression of inserted transgenes in cell lines. This new vector will be a useful tool for gene transfer in human and non-human cell lines, including gene therapy studies.
authors.affiliation: Division of Infectious Diseases, Center for Virus Research and Institute for Immunology, University of California, Irvine, California, 92697-4068, USA.
authors.forename: Daniel
authors.initials: D
authors.lastname: Cervantes-Garcia
authors.forename: Augusto
authors.initials: A
authors.lastname: Rojas-Martinez
authors.forename: David
authors.initials: D
authors.lastname: Camerini
mesh_terms: D002460:Cell Line; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods; D005822:Genetic Vectors; D006801:Humans; D019065:Virus Assembly; D058008:Xenotropic murine leukemia virus-related virus / Q000235:genetics*