title: All functional aquaporin-4 isoforms are expressed in the rat cochlea and contribute to the formation of orthogonal arrays of particles.
abstract: The water channel aquaporin-4 (AQP4) is expressed in the cochlea and is essential for normal hearing. Unlike other AQPs, multiple isoforms of AQP4 have been reported in diverse tissues, three of which, M1, M23, and Mz, function as water channels. In addition, these protein isoforms are found in higher order complexes. Morphologically these higher order complexes correspond to orthogonal arrays of particles (OAPs) that are found in cell membranes by freeze fracture analysis. Using RT-PCR, quantitative PCR and blue-native PAGE immunoblots we identified all functional AQP4 isoforms -M1, M23, and Mz- and the formation of higher-order complexes in the organ of Corti of the rat. Complementary freeze-fracture studies revealed OAPs distributed in the lateral and basal membrane domains of the cochlear duct supporting cells, specifically Hensen's cells and outer sulcus cells. The unique inter- and intracellular heterogeneity in size, density and shape of OAPs suggests exceptional physiological requirements for the maintenance of water homeostasis during auditory sensory transduction in the cochlea.
authors.affiliation: Institute of Anatomy, University of Tübingen, Österbergstraβe 3, 72074 Tübingen, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Hirt
authors.forename: C
authors.initials: C
authors.lastname: Gleiser
authors.forename: A
authors.initials: A
authors.lastname: Eckhard
authors.forename: A F
authors.initials: AF
authors.lastname: Mack
authors.forename: M
authors.initials: M
authors.lastname: Müller
authors.forename: H
authors.initials: H
authors.lastname: Wolburg
authors.forename: H
authors.initials: H
authors.lastname: Löwenheim
mesh_terms: D000818:Animals; D051401:Aquaporin 4 / Q000235:genetics / Q000378:metabolism*; D003051:Cochlea / Q000166:cytology / Q000378:metabolism*; D003053:Cochlear Duct / Q000166:cytology / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D005614:Freeze Fracturing; D009925:Organ of Corti / Q000166:cytology / Q000378:metabolism; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D059368:RNA Isoforms / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: C496032:Aqp4 protein, rat; D051401:Aquaporin 4; D020033:Protein Isoforms; D059368:RNA Isoforms; D012333:RNA, Messenger