title: Alert-type biological dosimeter based on enzyme logic system.
abstract: A cooperative effect of two biomarkers, α-amylase and lactate dehydrogenase, was used to analyze radiation-caused tissue damage in vitro in model solutions of human serum. The analytical system was based on the recently emerged biocomputing concept applying biocatalytic cascades for logic processing of biochemical input signals. The studied system resembled a Boolean NAND logic gate in which the change of the optical output signal from a high level (logic value 1) to a low level (logic value 0) confirmed the presence of both biomarkers at pathological concentrations (1,1 input signals), thus yielding the conclusion about radiation tissue damage. The system operates in a digital YES/NO format as an alert-type biosensor with a built-in Boolean logic.
authors.affiliation: Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY 13699-5810, USA.
authors.forename: Vera
authors.initials: V
authors.lastname: Bocharova
authors.forename: Jan
authors.initials: J
authors.lastname: Halámek
authors.forename: Jian
authors.initials: J
authors.lastname: Zhou
authors.forename: Guinevere
authors.initials: G
authors.lastname: Strack
authors.forename: Joseph
authors.initials: J
authors.lastname: Wang
authors.forename: Evgeny
authors.initials: E
authors.lastname: Katz
mesh_terms: D015415:Biomarkers / Q000032:analysis / Q000097:blood; D015374:Biosensing Techniques / Q000379:methods; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000032:analysis* / Q000097:blood; D008128:Logic; D008954:Models, Biological; D011832:Radiation Injuries / Q000175:diagnosis*; D011874:Radiometry / Q000295:instrumentation / Q000379:methods*; D044967:Serum / Q000737:chemistry*; D000516:alpha-Amylases / Q000032:analysis* / Q000097:blood
chemical_list: D015415:Biomarkers; D007770:L-Lactate Dehydrogenase; D000516:alpha-Amylases