title: Rhamnetin production based on the rational design of the poplar O-methyltransferase enzyme and its biological activities.
abstract: To produce rhamnetin using enzymatic engineering, poplar O-methyltransferase-7 and its mutants were prepared based on the rational enzyme design, and the production of rhamnetin was compared with the results obtained using the wild type enzyme. In addition, the potential of using rhamnetin as a cancer chemopreventive agent was compared with that of quercetin in MDA-MB-231 human breast cancer cells, and their bioavailabilities were tested in Dulbecco's modified Eagle's medium.
authors.affiliation: Division of Bioscience and Biotechnology, BMIC, RCD, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Sunhee
authors.initials: S
authors.lastname: Lee
authors.forename: Soon Young
authors.initials: SY
authors.lastname: Shin
authors.forename: Younggiu
authors.initials: Y
authors.lastname: Lee
authors.forename: Younghee
authors.initials: Y
authors.lastname: Park
authors.forename: Bong Gyu
authors.initials: BG
authors.lastname: Kim
authors.forename: Joong-Hoon
authors.initials: JH
authors.lastname: Ahn
authors.forename: Youhoon
authors.initials: Y
authors.lastname: Chong
authors.forename: Young Han
authors.initials: YH
authors.lastname: Lee
authors.forename: Yoongho
authors.initials: Y
authors.lastname: Lim
mesh_terms: D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000188:drug therapy*; D045744:Cell Line, Tumor; D015195:Drug Design; D005260:Female; D006801:Humans; D008780:Methyltransferases / Q000737:chemistry* / Q000235:genetics / Q000494:pharmacology*; D015394:Molecular Structure; D009154:Mutation; D032107:Populus / Q000201:enzymology*; D015202:Protein Engineering; D011794:Quercetin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000970:Antineoplastic Agents; C063423:rhamnetin; D011794:Quercetin; D008780:Methyltransferases