title: Cationic polyelectrolytes-lipases complexes formation as tool for recovery of these enzymes from their natural sources.
abstract: In the present paper the formation of complexes between positively charge polyelectrolyte (polyethyleneimine and chitosan) and Candida rugosa lipase from a crude extract and porcine lipase from pancreas commercial homogenate preparations were analyzed. The solubility of lipases-cationic polyelectrolytes formation was dependent on: polyelectrolyte densities electrical charge, polyelectrolyte and enzyme concentrations and salts present in the solution. The lipase was recovered from the non-soluble complex by adding of NaCl at a given pH. Although the polyelectrolytes did not affect lipase biological activity, both of them produced good enzyme recovery (>90%); however, purification factors were low. This methodology appears to be a good previous prepurification and concentration method, using, low-cost polymers, allows the design of a purification method where the protein of interest is present in a large volume with respect to the small amount of polyelectrolyte added.
authors.affiliation: Physical-Chemistry Department, National University of Rosario, Rosario, Argentina. gbassani@fbioyf.unr.edu.ar
authors.forename: Georgina
authors.initials: G
authors.lastname: Bassani
authors.forename: Beatriz
authors.initials: B
authors.lastname: Farruggia
authors.forename: Guillermo
authors.initials: G
authors.lastname: Picó
mesh_terms: D000818:Animals; D002175:Candida / Q000201:enzymology; D005591:Chemical Fractionation / Q000379:methods*; D011232:Chemical Precipitation; D048271:Chitosan / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D011094:Polyethyleneimine / Q000378:metabolism*; D011485:Protein Binding; D012995:Solubility; D013552:Swine; D014867:Water / Q000737:chemistry
chemical_list: D014867:Water; D011094:Polyethyleneimine; D048271:Chitosan; D008049:Lipase