title: Perturbation of the stability of amyloid fibrils through alteration of electrostatic interactions.
abstract: The self-assembly of proteins and peptides into polymeric amyloid fibrils is a process that has important implications ranging from the understanding of protein misfolding disorders to the discovery of novel nanobiomaterials. In this study, we probe the stability of fibrils prepared at pH 2.0 and composed of the protein insulin by manipulating electrostatic interactions within the fibril architecture. We demonstrate that strong electrostatic repulsion is sufficient to disrupt the hydrogen-bonded, cross-β network that links insulin molecules and ultimately results in fibril dissociation. The extent of this dissociation correlates well with predictions for colloidal models considering the net global charge of the polypeptide chain, although the kinetics of the process is regulated by the charge state of a single amino acid. We found the fibrils to be maximally stable under their formation conditions. Partial disruption of the cross-β network under conditions where the fibrils remain intact leads to a reduction in their stability. Together, these results support the contention that a major determinant of amyloid stability stems from the interactions in the structured core, and show how the control of electrostatic interactions can be used to characterize the factors that modulate fibril stability.
authors.affiliation: Department of Chemistry, University of Cambridge, Cambridge, United Kingdom. sls42@cam.ac.uk
authors.forename: Sarah L
authors.initials: SL
authors.lastname: Shammas
authors.forename: Tuomas P J
authors.initials: TP
authors.lastname: Knowles
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Baldwin
authors.forename: Cait E
authors.initials: CE
authors.lastname: Macphee
authors.forename: Mark E
authors.initials: ME
authors.lastname: Welland
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Dobson
authors.forename: Glyn L
authors.initials: GL
authors.lastname: Devlin
mesh_terms: D000595:Amino Acid Sequence; D000682:Amyloid / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007328:Insulin / Q000737:chemistry*; D007700:Kinetics; D008969:Molecular Sequence Data; D055503:Protein Multimerization; D055550:Protein Stability; D017433:Protein Structure, Secondary; D055672:Static Electricity; D013816:Thermodynamics
chemical_list: D000682:Amyloid; D007328:Insulin