title: False-negative results using Neisseria gonorrhoeae porA pseudogene PCR - a clinical gonococcal isolate with an N. meningitidis porA sequence, Australia, March 2011.
abstract: The gonococcal porA pseudogene is a popular target for in-house Neisseria gonorrhoeae PCR methods. With this study we present two novel findings: the first case of an N. gonorrhoeae porA pseudogene PCR false-negative result caused by sequence variation, and in the same organism, the first description of a clinical N. gonorrhoeae strain harbouring an N. meningitidis porA sequence.
authors.affiliation: Queensland Paediatric Infectious Diseases Laboratory, Queensland Children s Health Services, Australia. d.whiley@uq.edu.au
authors.forename: D M
authors.initials: DM
authors.lastname: Whiley
authors.forename: A
authors.initials: A
authors.lastname: Limnios
authors.forename: N J
authors.initials: NJ
authors.lastname: Moon
authors.forename: N
authors.initials: N
authors.lastname: Gehrig
authors.forename: N
authors.initials: N
authors.lastname: Goire
authors.forename: T
authors.initials: T
authors.lastname: Hogan
authors.forename: A
authors.initials: A
authors.lastname: Lam
authors.forename: K
authors.initials: K
authors.lastname: Jacob
authors.forename: S B
authors.initials: SB
authors.lastname: Lambert
authors.forename: M D
authors.initials: MD
authors.lastname: Nissen
authors.forename: T P
authors.initials: TP
authors.lastname: Sloots
mesh_terms: D001483:Base Sequence; D005188:False Negative Reactions; D006069:Gonorrhea / Q000175:diagnosis / Q000235:genetics* / Q000382:microbiology*; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D009344:Neisseria gonorrhoeae / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D018272:Porins / Q000235:genetics*; D055815:Young Adult
chemical_list: D018272:Porins; C057699:porin protein, Neisseria