title: Isolation and characterization of emerging subgroup J avian leukosis virus associated with hemangioma in egg-type chickens.
abstract: Subgroup J avian leukosis virus (ALV-J), first isolated in 1989, predominantly causes myeloid leukosis (ML) in meat-type or egg-type chicken. Since 2006, the clinical cases of hemangioma rather than ML in commercial layer flocks associated with ALV-J have been reported, but it was still not clear whether the novel oncogenic ALV-J had emerged. We characterized SCAU-HN06 isolate of ALV-J from hemangioma in commercial Roman layers through animal experiment and full-length proviral genome sequence analysis. The SPF white leghorn egg-type chickens infected with SCAU-HN06 in ovo at day 11 of incubation showed an overall incidence of 56% hemangioma and 8% renal tumor throughout the 22-week trial, the mortality rate was 16%. Most genes of SCAU-HN06 isolate showed high nucleotide sequence identity to JS09GY6 which was isolated from Hy-Line Variety Brown layers suffering hemangioma. The 19-bp insertion in leader sequence and one key deletion in E element were the common features of SCAU-HN06 and JS09GY6. SCAU-HN06 and those ALV-Js associated with hemangioma, possibly recombinants of ALV-J and other avian retrovirus, may share the same ancestor.
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, 483 Wushan Street, Tianhe District, Guangzhou, Guangdong Province 510642, People's Republic of China.
authors.forename: Hanzhang
authors.initials: H
authors.lastname: Lai
authors.forename: Henan
authors.initials: H
authors.lastname: Zhang
authors.forename: Zhangyong
authors.initials: Z
authors.lastname: Ning
authors.forename: Ruiai
authors.initials: R
authors.lastname: Chen
authors.forename: Wenyan
authors.initials: W
authors.lastname: Zhang
authors.forename: Aijian
authors.initials: A
authors.lastname: Qing
authors.forename: Chaoan
authors.initials: C
authors.lastname: Xin
authors.forename: Kangzhen
authors.initials: K
authors.lastname: Yu
authors.forename: Weisheng
authors.initials: W
authors.lastname: Cao
authors.forename: Ming
authors.initials: M
authors.lastname: Liao
mesh_terms: D000818:Animals; D001353:Avian Leukosis / Q000473:pathology / Q000821:virology*; D001354:Avian Leukosis Virus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D001483:Base Sequence; D002645:Chickens / Q000821:virology*; D004279:DNA, Viral / Q000235:genetics; D016679:Genome, Viral; D006391:Hemangioma / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D004279:DNA, Viral