title: Inhibitory effect of a glycoprotein isolated from golden oyster mushroom ( Pleurotus citrinopileatus ) on the lipopolysaccharide-induced inflammatory reaction in RAW 264.7 macrophage.
abstract: Mushrooms have become an important source of natural antitumor, antiviral, antibacterial, immunomodulatory, and anti-inflammatory agents. Golden oyster mushroom, Pleurotus citrinopileatus , is a common mushroom in oriental countries for human consumption. The present study investigated the anti-inflammatory reaction of the bioactive nonlectin glycoprotein (PCP-3A) isolated from the fresh fruiting body of this mushroom. Western blot analysis on LPS-induced iNOS, COX-2, and NF-κB expressions in RAW 264.7 cells as affected by PCP3-A was performed to elucidate the mechanism of NO and PGE2 reduction. The results showed that PCP-3A failed to affect RAW 264.7 viability at a concentration up to 6.25 μg/mL, but inhibited LPS (1 μg/mL)-induced expression, and that PCP-3A inhibited the production of NO and PGE2 in LPS-activated macrophages via the down-regulation of certain pro-inflammatory mediators, including iNOS and NF-κB.
authors.affiliation: Graduate Institute of Food Science and Technology, National Taiwan University , Taipei 106, Taiwan.
authors.forename: Jian-Nan
authors.initials: JN
authors.lastname: Chen
authors.forename: Elvira Gonzalez
authors.initials: EG
authors.lastname: de Mejia
authors.forename: James Swi-Bea
authors.initials: JS
authors.lastname: Wu
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D048690:Fruiting Bodies, Fungal / Q000737:chemistry; D006023:Glycoproteins / Q000302:isolation & purification / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D020076:Pleurotus / Q000737:chemistry*
chemical_list: D000893:Anti-Inflammatory Agents; D006023:Glycoproteins; D008070:Lipopolysaccharides