title: The expression and role of c-Myc in mouse hair follicle morphogenesis and cycling.
abstract: Although the function of c-Myc has been clarified in many tissues, until now its expression and role in hair follicle morphogenesis and the hair cycle remains unknown. In this study we detected c-Myc expression pattern in the process of mouse hair follicle development and normal cycle. We found that during hair follicle morphogenesis, the stage-specific expression of c-Myc was detected in mouse skin and was predominantly localized to the hair follicle epithelium. c-Myc expression was also consistently found in mouse skin throughout the hair follicle cycle. Through the in vivo injection of c-Myc inhibitory peptide and c-Myc expression plasmid, we also investigated the direct effects of c-Myc on the hair follicle structures during the hair follicle cycle. Our results showed that c-Myc inhibitory peptide significantly restrained the development of anagen hair follicles, while the injection of plasmid DNA encoding c-Myc in vivo clearly promoted anagen development. Our data indicate that c-Myc may play an important role in the proliferation and differentiation of the hair follicle keratinocytes during hair follicle development. c-Myc also was shown to participate in the regulation of the mouse hair growth cycle and could promote the proliferation of the hair matrix keratinocytes as well as the differentiation of the inner root sheath.
authors.affiliation: Department of Cell Biology, Third Military Medical University, Sha Pingba, Chongqing, PR China.
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.forename: Tian
authors.initials: T
authors.lastname: Yang
authors.forename: Jin
authors.initials: J
authors.lastname: Li
authors.forename: Mingxing
authors.initials: M
authors.lastname: Lei
authors.forename: Jiazhong
authors.initials: J
authors.lastname: Shi
authors.forename: Weiming
authors.initials: W
authors.lastname: Qiu
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Lian
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation / Q000187:drug effects; D004622:Embryo, Mammalian; D004848:Epithelium / Q000196:embryology / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental; D020868:Gene Silencing; D018859:Hair Follicle / Q000196:embryology / Q000378:metabolism* / Q000648:ultrastructure; D007150:Immunohistochemistry; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D009024:Morphogenesis; D010455:Peptides / Q000494:pharmacology; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism*; D012879:Skin Physiological Phenomena / Q000235:genetics
chemical_list: C489428:Myc protein, mouse; D010455:Peptides; D016271:Proto-Oncogene Proteins c-myc