title: Microarray analysis of differentially expressed background genes in rats following hemorrhagic shock.
abstract: To uncover the contribution of the diversity of the genetic backgrounds to the pathogenesis of hemorrhagic shock, we employed male Sprague-Dawley rats to establish a controlled 2.5 ml/100 g total body weight fixed-volume hemorrhagic shock and left lobular hepatectomy model. RNA was isolated from the liver samples taken from the rats (survival group: rats survived over 24 h after shock; and dead group: rats died within 1 h after shock, n = 3 per group), and subjected to microarray using the illumina(TM) chips for rat cDNA (27,342 genes, >700,000 probes). The results demonstrated that the rats had about 50% survival rate and 100 genes were identified differentially expressed in the two groups. Of these genes, 47 genes were up-regulated and 53 genes down-regulated. Real-time PCR confirmed the differential expression for Aldh1a1, Aldh1a7, Aoc3, Cyp26al, Hdc and Ephx2 genes. Pathway analysis revealed that these genes are involved in circadian rhythm, beta-Alanine metabolism, histidine metabolism, biosynthesis of unsaturated fatty acids, glycine, serine and threonine metabolism, vitamin B6 metabolism, as well as arginine and proline metabolism. Therefore, our study provided a global molecular view on the contribution of genetic backgrounds to the response to hemorrhagic shock.
authors.affiliation: Department of General Surgery, Changzheng Hospital Affiliated To Second Military Medical University, 415# Fengyang Road, 200003 Shanghai, People's Republic of China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Xiaojun
authors.forename: Qian
authors.initials: Q
authors.lastname: Cheng
authors.forename: Zhang
authors.initials: Z
authors.lastname: Yuxing
authors.forename: Hu
authors.initials: H
authors.lastname: Zhiqian
mesh_terms: D000080924:Aldehyde Dehydrogenase 1 Family; D006631:Amine Oxidase (Copper-Containing) / Q000235:genetics / Q000378:metabolism; D000818:Animals; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D004851:Epoxide Hydrolases / Q000235:genetics / Q000378:metabolism; D005453:Fluorescence; D005786:Gene Expression Regulation / Q000235:genetics*; D014644:Genetic Variation; D006498:Hepatectomy; D008099:Liver / Q000378:metabolism*; D008297:Male; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D046228:Microarray Analysis; D012313:RNA / Q000235:genetics* / Q000302:isolation & purification; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D050697:Retinal Dehydrogenase / Q000235:genetics / Q000378:metabolism; D000072516:Retinoic Acid 4-Hydroxylase; D012771:Shock, Hemorrhagic / Q000378:metabolism*; D016019:Survival Analysis
chemical_list: D015815:Cell Adhesion Molecules; D017931:DNA Primers; D012313:RNA; D003577:Cytochrome P-450 Enzyme System; D000072516:Retinoic Acid 4-Hydroxylase; D000080924:Aldehyde Dehydrogenase 1 Family; C523119:Aldh1a1 protein, rat; D050697:Retinal Dehydrogenase; D006631:Amine Oxidase (Copper-Containing); C510433:vascular adhesion protein-1, rat; D004851:Epoxide Hydrolases; C529022:EPHX2 protein, rat