title: Dielectrophoresis-based cellular microarray chip for anticancer drug screening in perfusion microenvironments.
abstract: We present a dielectrophoresis (DEP)-based cellular microarray chip for cell-based anticancer drug screening in perfusion microenvironments. Human breast cancer cells, MCF7, were seeded into the chip and patterned via DEP forces onto the planar interdigitated ring electrode (PIRE) arrays. Roughly, only one third of the cell amount was required for the chip compared to that for a 96-well plate control. Drug concentrations (cisplatin or docetaxel) were stably generated by functional integration of a concentration gradient generator (CGG) and an anti-crosstalk valve (ACV) to treat cells for 24 hours. Cell viability was quantified using a dual staining method. Results of cell patterning show substantial uniformity of patterned cells (92 ± 5 cells per PIRE). Furthermore, after 24 hour drug perfusion, no statistical significance in dose-responses between the chip and the 96-well plate controls was found. The IC(50) value from the chip also concurred with the values from the literature. Moreover, the perfusion culture exhibited reproducibility of drug responses of cells on different PIREs in the same chamber. The chip would enable applications where cells are of limited supply, and supplement microfluidic perfusion cultures for clinical practices.
authors.affiliation: Institute of Applied Mechanics, National Taiwan University, Taipei, Taiwan.
authors.forename: Lo-Chang
authors.initials: LC
authors.lastname: Hsiung
authors.forename: Chi-Ling
authors.initials: CL
authors.lastname: Chiang
authors.forename: Chen-Ho
authors.initials: CH
authors.lastname: Wang
authors.forename: Yu-Hsu
authors.initials: YH
authors.lastname: Huang
authors.forename: Ching-Te
authors.initials: CT
authors.lastname: Kuo
authors.forename: Ji-Yen
authors.initials: JY
authors.lastname: Cheng
authors.forename: Ching-Hung
authors.initials: CH
authors.lastname: Lin
authors.forename: Victoria
authors.initials: V
authors.lastname: Wu
authors.forename: Hsien-Yeh
authors.initials: HY
authors.lastname: Chou
authors.forename: De-Shien
authors.initials: DS
authors.lastname: Jong
authors.forename: Hsinyu
authors.initials: H
authors.lastname: Lee
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Wo
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000633:toxicity*; D001943:Breast Neoplasms / Q000188:drug therapy; D045744:Cell Line, Tumor; D002470:Cell Survival; D002945:Cisplatin / Q000737:chemistry / Q000633:toxicity; D000077143:Docetaxel; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D004566:Electrodes; D004586:Electrophoresis / Q000295:instrumentation / Q000379:methods*; D005260:Female; D006801:Humans; D046228:Microarray Analysis / Q000295:instrumentation / Q000379:methods*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods; D043823:Taxoids / Q000737:chemistry / Q000633:toxicity
chemical_list: D000970:Antineoplastic Agents; D043823:Taxoids; D000077143:Docetaxel; D002945:Cisplatin