title: Nucleobase modification as redox DNA labelling for electrochemical detection.
abstract: Basic aspects of DNA electrochemistry with a strong focus on the use of modified nucleobases as redox probes for electrochemical bioanalysis are reviewed. Intrinsic electrochemical properties of nucleobases in combination with artificial redox-active nucleobase modifications are frequently applied in this field. Synthetic approaches (both chemical and enzymatic) to base-modified nucleic acids are briefly summarized and their applications in redox labelling are discussed. Finally, analytical applications including DNA hybridization, primer extension, PCR, SNP typing, DNA damage and DNA-protein interaction analysis are presented (critical review, 91 references).
authors.affiliation: Institute of Organic Chemistry and Biochemistry, v.v.i., Academy of Sciences of the Czech Republic, Gilead Sciences & IOCB Research Center, Prague, Czech Republic. hocek@uochb.cas.cz
authors.forename: Michal
authors.initials: M
authors.lastname: Hocek
authors.forename: Miroslav
authors.initials: M
authors.lastname: Fojta
mesh_terms: D004247:DNA / Q000032:analysis* / Q000737:chemistry* / Q000235:genetics; D004563:Electrochemistry / Q000379:methods*; D006801:Humans; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D010084:Oxidation-Reduction; D013194:Staining and Labeling
chemical_list: D009838:Oligodeoxyribonucleotides; D004247:DNA