title: Biochemical responses and DNA damage in red sea bream from coastal Fujian Province, China.
abstract: Relationships between measures of oxidative stress and adaptive antioxidant defenses were studied in red sea bream (Pagrus major, RSB) caged at eight locations along the Fujian coast, China. Oxidative damage in tissues was investigated by measuring concentrations of protein carbonyl (CP) and reactive oxygen species (ROS), lipid peroxidation (LPO), acetyl cholinesterase activity (AChE), concentration of thiol groups and activities of enzymes associated with antioxidant defense. Magnitudes of antioxidant defense parameters were significantly, positively correlated with oxidative stress. Significantly greater ROS, LPO and CP content was observed in most tissues of RSB caged at three locations relative to those at the other locations. RSB caged at one location, Meizhou, exhibited significantly more DNA damage than did RSB caged at other locations. AChE activities in liver, kidney and muscle were statistically different among locations. Multivariate analyses were used to categorize the environmental quality of different locations. The battery of measures of oxidative stress proved to be a useful integrator of oxidative stresses that could be compared among locations.
authors.affiliation: State Key Laboratory of Marine Environmental Science, College of Oceanography and Environmental Science, Xiamen University, Xiamen 361005, China.
authors.forename: Singaram
authors.initials: S
authors.lastname: Gopalakrishnan
authors.forename: Zhang
authors.initials: Z
authors.lastname: Nai
authors.forename: Harikrishnan
authors.initials: H
authors.lastname: Thilagam
authors.forename: Chen
authors.initials: C
authors.lastname: Bei
authors.forename: Jian
authors.initials: J
authors.lastname: Ding
authors.forename: Xin-Hong
authors.initials: XH
authors.lastname: Wang
authors.forename: Wen-Xiong
authors.initials: WX
authors.lastname: Wang
authors.forename: Cai-Huan
authors.initials: CH
authors.lastname: Ke
authors.forename: John P
authors.initials: JP
authors.lastname: Giesy
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Zhang
authors.forename: Ke-Jian
authors.initials: KJ
authors.lastname: Wang
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D002374:Catalase / Q000378:metabolism; D002681:China; D004249:DNA Damage; D004784:Environmental Monitoring; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D021541:Sea Bream / Q000502:physiology*; D013482:Superoxide Dismutase / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D017382:Reactive Oxygen Species; D014874:Water Pollutants, Chemical; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; D000110:Acetylcholinesterase; D005978:Glutathione