title: Establishment of an Atlantic salmon kidney cell line with an inducible gene expression system.
abstract: A stable recombinant Atlantic Salmon Kidney cell line ASK for use as an inducible expression system was isolated, cloned and characterised. The cells were transfected with the pTet-Off plasmid from the Tet On/Off Clontech system, carrying a G418 resistance gene. Several G418-resistant clones were sub-cultured and characterised by qPCR and by transient transfection. The level of expression of transcriptional activator (tTA) was measured by qPCR in a number of isolated clones. Transient transfection with a pTRE2-hyg-LUC plasmid was used to evaluate the inducibility of these clones. Two clones were chosen for their compromise between cell growth and inducibility. This genetically engineered cell line is a valuable tool for the fish research community especially in research areas investigating the biological function of viral proteins.
authors.affiliation: Marine Scotland, Marine Laboratory, 375 Victoria Road, Aberdeen AB11 9DB, UK. Bertrand.Collet@scotland.gsi.gov.uk
authors.forename: Bertrand
authors.initials: B
authors.lastname: Collet
authors.forename: Katherine
authors.initials: K
authors.lastname: Lester
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D007668:Kidney / Q000166:cytology*; D020317:Salmo salar; D014162:Transfection