title: Potential role of P-gp for flavone-induced diminished apoptosis and increased adenoma size in the small intestine of APC(min/+) mice.
abstract: APC(min/+) mice, carrying a nonsense mutation in the adenomatous polyposis coli (APC) gene, appear as a perfect model to study development or therapy of intestinal neoplasia. We tested whether the flavonoid flavone is able to affect adenoma development in APC(min/+) mice. Tumor sizes were significantly increased by flavone selectively in small intestine. This was associated with reduced cell numbers displaying cleaved caspase-3 and enhanced expression of phosphoglycoprotein (P-gp). However, according to great variability in P-gp expression in all parts of mice intestines, an association between expression of P-gp and inhibition of apoptosis was demonstrated in human Caco-2 colorectal cancer cells.
authors.affiliation: Molecular Nutrition Research, Interdisciplinary Research Center, Justus-Liebig-University of Giessen, Heinrich-Buff-Ring, Germany.
authors.forename: Marco
authors.initials: M
authors.lastname: Schumacher
authors.forename: Anja
authors.initials: A
authors.lastname: Hautzinger
authors.forename: Annette
authors.initials: A
authors.lastname: Rossmann
authors.forename: Susanne
authors.initials: S
authors.lastname: Holzhauser
authors.forename: Daniela
authors.initials: D
authors.lastname: Popovic
authors.forename: Anke
authors.initials: A
authors.lastname: Hertrampf
authors.forename: Doris
authors.initials: D
authors.lastname: Oesterle
authors.forename: Carmen
authors.initials: C
authors.lastname: Spiller
authors.forename: Michael
authors.initials: M
authors.lastname: Boll
authors.forename: Uwe
authors.initials: U
authors.lastname: Wenzel
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000502:physiology*; D000236:Adenoma / Q000473:pathology*; D025601:Adenomatous Polyposis Coli Protein / Q000235:genetics / Q000378:metabolism; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D018938:Caco-2 Cells; D005260:Female; D047309:Flavones / Q000494:pharmacology*; D017491:Genes, APC / Q000502:physiology*; D006801:Humans; D007082:Ileum / Q000187:drug effects / Q000473:pathology; D007414:Intestinal Neoplasms / Q000473:pathology*; D007583:Jejunum / Q000187:drug effects / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D051176:beta Catenin / Q000502:physiology
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D025601:Adenomatous Polyposis Coli Protein; D047309:Flavones; D051176:beta Catenin