title: Study on cow ghee versus soybean oil on 7,12-dimethylbenz(a)-anthracene induced mammary carcinogenesis & expression of cyclooxygenase-2 & peroxisome proliferators activated receptor-γ in rats.
abstract: OBJECTIVE
  Breast cancer is a leading cause of cancer death in women; dietary fat is the one of the factors that influences its incidence. In the present study we investigated the effect of feeding cow ghee versus soybean oil on 7,12-dimethylbenz(a)anthracene (DMBA) induced mammary cancer in rat and expression of cyclooxygenase-2 and peroxisome proliferators activated receptor-γ (PPAR-γ) in mammary gland.
  
  
  METHODS
  Two groups of 21 day old female rats (30 each) were fed for 44 wk diet containing cow ghee or soybean oil (10%). The animals were given DMBA (30 mg/kg body weight) through oral intubation after 5 wk feeding. Another two groups (8 each) fed similarly but not given DMBA served as control for the gene expression study.
  
  
  RESULTS
  In DMBA treated groups, the animal fed soybean oil had higher tumour incidence (65.4%), tumour weight (6.18 g) and tumour volume (6285 mm3) compared to those fed cow ghee (26.6%, 1.67 g, 1925 mm3, respectively). Tumour latency period was 23 wk on soybean oil compared to 27 wk on cow ghee. Histological analysis of tumours showed that the progression of carcinogenesis was more rapid on soybean oil than on cow ghee. The expression of cyclooxygenase-2 was observed only in DMBA treated rats and it was significantly less on cow ghee than on soybean oil. The expression of PPAR-γ was significantly more on cow ghee than on soybean oil.
  
  
  CONCLUSIONS
  Our results show that dietary cow ghee opposed to soybean oil attenuates mammary carcinogenesis induced by DMBA; and the effect is mediated by decreased expression of cyclooxygenase-2 and increased expression of PPAR-γ in the former group.
authors.affiliation: Division of Animal Biochemistry, National Dairy Research Institute, Karnal, Haryana, India.
authors.forename: Rita
authors.initials: R
authors.lastname: Rani
authors.forename: Vinod K
authors.initials: VK
authors.lastname: Kansal
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001551:Benz(a)Anthracenes / Q000008:administration & dosage / Q000633:toxicity*; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D017931:DNA Primers / Q000235:genetics; D004041:Dietary Fats / Q000494:pharmacology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D008325:Mammary Neoplasms, Experimental / Q000139:chemically induced* / Q000378:metabolism; D047495:PPAR gamma / Q000378:metabolism*; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013024:Soybean Oil / Q000494:pharmacology
chemical_list: D001551:Benz(a)Anthracenes; D017931:DNA Primers; D004041:Dietary Fats; D047495:PPAR gamma; C046810:7,12-dihydroxymethylbenz(a)anthracene; D013024:Soybean Oil; D051546:Cyclooxygenase 2