title: Single-shot optical sectioning using two-color probes in HiLo fluorescence microscopy.
abstract: We describe a wide-field fluorescence microscope setup which combines HiLo microscopy technique with the use of a two-color fluorescent probe. It allows one-shot fluorescence optical sectioning of thick biological moving sample which is illuminated simultaneously with a flat and a structured pattern at two different wavelengths. Both homogenous and structured fluorescence images are spectrally separated at detection and combined similarly with the HiLo microscopy technique. We present optically sectioned full-field images of Xenopus laevis embryos acquired at 25 images/s frame rate.
authors.affiliation: Laboratoire de Physique et d'Étude des Matériaux, Centre National de la Recherche Scientifique, UMR 8213, École Supérieure de Physique et de Chimie Industrielles, Paris, France.
authors.forename: Eleonora
authors.initials: E
authors.lastname: Muro
authors.forename: Pierre
authors.initials: P
authors.lastname: Vermeulen
authors.forename: Andriani
authors.initials: A
authors.lastname: Ioannou
authors.forename: Paris
authors.initials: P
authors.lastname: Skourides
authors.forename: Benoit
authors.initials: B
authors.lastname: Dubertret
authors.forename: Alexandra
authors.initials: A
authors.lastname: Fragola
authors.forename: Vincent
authors.initials: V
authors.lastname: Loriette
mesh_terms: D000818:Animals; D003116:Color; D005456:Fluorescent Dyes / Q000737:chemistry*; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D008863:Microspheres; D055620:Optical Phenomena; D014982:Xenopus laevis / Q000196:embryology
chemical_list: D005456:Fluorescent Dyes