title: RNA synthesis by reverse direction process: phosphoramidites and high purity RNAs and introduction of ligands, chromophores, and modifications at 3'-end.
abstract: We have synthesized and studied the coupling properties of 3'-DMT-5'-CE phosphoramidites. The coupling efficiency per step surpasses 99% in the reverse-direction synthesis methodology, leading to high-purity RNA in a large number of 20- to 21-mers and long-chain oligonucleotides. Our data show that 5'→3' direction synthesis has a distinct advantage compared to the conventional method. As a result, this method of RNA synthesis is expected to be a very useful and practical method of choice for therapeutic-grade RNA. The phosphoramidites, Rev-A-n-bz, Rev-C-n-bz, Rev-C-n-ac, Rev-G-n-ac, and Rev-rU are routinely produced with an HPLC purity of greater than 98% and (31)P NMR purity greater than 99.5%.
authors.affiliation: ChemGenes Corp., Wilmington, Massachusetts, USA.
authors.forename: Suresh C
authors.initials: SC
authors.lastname: Srivastava
authors.forename: Divya
authors.initials: D
authors.lastname: Pandey
authors.forename: Naveen P
authors.initials: NP
authors.lastname: Srivastava
authors.forename: Satya P
authors.initials: SP
authors.lastname: Bajpai
mesh_terms: D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D008024:Ligands; D015394:Molecular Structure; D009843:Oligoribonucleotides / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000737:chemistry*; D017646:Organosilicon Compounds / Q000737:chemistry*; D012313:RNA / Q000138:chemical synthesis*; D017423:Sequence Analysis, RNA
chemical_list: D008024:Ligands; D009843:Oligoribonucleotides; D009943:Organophosphorus Compounds; D017646:Organosilicon Compounds; C434331:phosphoramidite; C009660:t-butyldimethylsilyl compounds; D012313:RNA