title: Anthelmintic screening of fractions of Elephantorrhiza elephantina root extract against Haemonchus contortus.
abstract: In the previous work conducted, it was found that the root extract of Elephantorrhiza elephantina (Bruch.) Skeels. exhibited good anthelmintic efficacy against eggs and larvae of Haemonchus contortus. This study was therefore undertaken to screen fractions of the extracts of E. elephantina for their anthelmintic activity against adult H. contortus using a bioactivity-guided assay. The adult worm bioassay was conducted using aqueous, hexane and ethyl acetate fractions of the plant extracts at concentrations of 0.312, 0.625, 1.25, 2.5, 5 and 10 mg/ml in vitro. Albendazole and distilled water were used as positive and negative control, respectively. In vitro treatment of adult worms revealed concentration and time-dependent efficacy of the fractions of E. elephantina. The aqueous and ethyl acetate fractions showed a highly significant (P<0.05) motility inhibition at concentrations of 2.5 mg/ml and above after 6 h of exposure, while the hexane fraction showed a significant (P<0.05) motility inhibition at concentrations of 5 mg/ml and above. After 30 h of exposure, all the fractions showed insignificant (P>0.05) inhibition of motility. The fractions of E. elephantina also showed mortality indexes that were not significantly different to each other, as well as to the commercial drug (albendazole). Overall, the ethyl acetate and the aqueous fractions were found to possess the highest anthelmintic activity. We therefore recommend future studies to further investigate effective dosages of these fractions in vivo, as well as a full investigation into the compounds responsible for the biological activity.
authors.affiliation: Department of Livestock and Pasture Sciences, Faculty of Science and Agriculture, University of Fort Hare, P/Bag X1314, Alice, 5700, Republic of South Africa.
authors.forename: Viola
authors.initials: V
authors.lastname: Maphosa
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: Masika
mesh_terms: D015766:Albendazole / Q000494:pharmacology; D000818:Animals; D000871:Anthelmintics / Q000737:chemistry / Q000494:pharmacology*; D001681:Biological Assay / Q000379:methods*; D007887:Fabaceae / Q000737:chemistry*; D015511:Goat Diseases / Q000188:drug therapy / Q000469:parasitology*; D006041:Goats; D006188:Haemonchiasis / Q000188:drug therapy / Q000469:parasitology / Q000662:veterinary*; D006189:Haemonchus / Q000187:drug effects* / Q000254:growth & development; D007814:Larva / Q000187:drug effects / Q000254:growth & development; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D013019:South Africa
chemical_list: D000871:Anthelmintics; D010936:Plant Extracts; D015766:Albendazole