title: A dual-beam optical microscope for observation and cleavage of single DNA molecules.
abstract: An integrated epifocal and evanescent-wave optical microscope has been developed for real-time observation and bond cleavage studies of single DNA molecules. Large genomic DNA is stretched in a laminar flow stream and is immobilized on a polylysine-coated glass surface by strong electrostatic interactions. Unlike previous single-molecule dynamics and single-enzyme studies, this work takes advantage of the elastic nature of double-stranded DNA and measures DNA relaxation events that are triggered by two phosphodiester (P-O) bond breaks. The ability to follow chemical reactions on individual DNA molecules opens new possibilities for DNA mapping and for studying DNA-protein interactions.
authors.affiliation: Department of Chemistry, Indiana University, Bloomington, Indiana 47405.
authors.forename: W A
authors.initials: WA
authors.lastname: Lyon
authors.forename: M M
authors.initials: MM
authors.lastname: Fang
authors.forename: W E
authors.initials: WE
authors.lastname: Haskins
authors.forename: S
authors.initials: S
authors.lastname: Nie