title: Effects of DMSA-coated Fe3O4 magnetic nanoparticles on global gene expression of mouse macrophage RAW264.7 cells.
abstract: Fe(3)O(4) magnetic nanoparticles (MNPs) coated with 2,3-dimercaptosuccinnic acid (DMSA) are considered to be a promising nanomaterial with biocompatibility. In the present study, the effects of DMSA-coated Fe(3)O(4) MNPs on the expression of all identified mouse genes, which regulate various cellular biological processes, were determined to establish whether this nanoparticle is cytotoxic to mammalian cells. Mouse macrophage RAW264.7 cells were treated with 100μg/ml of DMSA-coated Fe(3)O(4) MNPs for 4, 24 and 48h, and the global gene expression was detected via Affymetrix Mouse Genome 430 2.0 GeneChips(®) microarrays. It was found that gene expression of 711, 545 and 434 transcripts was significantly altered by 4-, 24- and 48-h treatments, respectively. Of these genes, 27 were consistently upregulated and 6 were consistently downregulated at the three treatment durations. Bioinformatic analysis of all differentially expressed genes revealed that this nanoparticle can strongly activate inflammatory and immune responses and can inhibit the biosynthesis and metabolism of RAW264.7 cells at a dose of 100μg/ml. These results demonstrated that DMSA-coated Fe(3)O(4) MNPs display cytotoxicity in this type of macrophage at high doses.
authors.affiliation: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
authors.forename: Yingxun
authors.initials: Y
authors.lastname: Liu
authors.forename: Zhongping
authors.initials: Z
authors.lastname: Chen
authors.forename: Ning
authors.initials: N
authors.lastname: Gu
authors.forename: Jinke
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003287:Contrast Media / Q000737:chemistry / Q000633:toxicity*; D015536:Down-Regulation; D004337:Drug Carriers / Q000737:chemistry*; D015870:Gene Expression / Q000187:drug effects*; D020869:Gene Expression Profiling; D008264:Macrophages / Q000187:drug effects* / Q000648:ultrastructure; D058185:Magnetite Nanoparticles / Q000737:chemistry / Q000633:toxicity*; D051379:Mice; D008854:Microscopy, Electron; D020411:Oligonucleotide Array Sequence Analysis; D010316:Particle Size; D004113:Succimer / Q000737:chemistry*; D013499:Surface Properties; D015854:Up-Regulation
chemical_list: D003287:Contrast Media; D004337:Drug Carriers; D058185:Magnetite Nanoparticles; D004113:Succimer