title: Nanoprobes: quantitatively detecting the femtogram level of arsenite ions in live cells.
abstract: In this report, nanoprobes which could detect the femtogram level of arsenite ions in subcellular organelle of live cells are disclosed. The nanoprobes are composed of ssDNA and single-wall carbon nanotubes (SWCNTs), and the ssDNA is marked by a dye molecule. In a live cell, trace arsenite ions could interact with nanoprobes and significantly decrease the emission of the nanoprobes. With the help of a confocal microscope and cryo-electron microscopy, the lysosome target of the arsenite ion and nanoprobe is well described in high spatial resolution.
authors.affiliation: CAS Key Lab for Biologic Effects of Nanomaterials and Nanosafety and Beijing Synchrotron Lab Institute of High Energy Physics, Chinese Academy of Science, Beijing 100049 PR China.
authors.forename: Ru
authors.initials: R
authors.lastname: Liu
authors.forename: Zhong
authors.initials: Z
authors.lastname: Chen
authors.forename: Yaling
authors.initials: Y
authors.lastname: Wang
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Cui
authors.forename: Huarui
authors.initials: H
authors.lastname: Zhu
authors.forename: Ping
authors.initials: P
authors.lastname: Huang
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Yuliang
authors.initials: Y
authors.lastname: Zhao
authors.forename: Ye
authors.initials: Y
authors.lastname: Tao
authors.forename: Xueyun
authors.initials: X
authors.lastname: Gao
mesh_terms: D018053:Arsenites / Q000378:metabolism*; D002470:Cell Survival; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D057230:Limit of Detection; D008247:Lysosomes / Q000378:metabolism; D049329:Nanostructures / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry
chemical_list: D018053:Arsenites; D004277:DNA, Single-Stranded; D005456:Fluorescent Dyes; D037742:Nanotubes, Carbon; C015001:arsenite