title: Cytotoxic alkaloids from stems, leaves and twigs of Dasymaschalon blumei.
abstract: Bioassay-guided fractionation of the cytotoxic ethyl acetate extract from the stems of Dasymaschalon blumei (Annonaceae) led to the isolation of four aristololactam alkaloids, including the hitherto unknown 3,5-dihydroxy-2,4-dimethoxyaristolactam (1), as well as the three known compounds, aristolactam BI, goniopedaline, and griffithinam. Additionally, the cytotoxic extract from the combined leaves and twigs of the same plant yielded three known oxoaporphine alkaloids, oxodiscoguattine, dicentrinone, and duguevalline. The structures of aristolactams and oxoaporphine alkaloids were elucidated on the basis of spectroscopic methods. All isolates were evaluated for cytotoxicity against a panel of mammalian cancer cell lines and a noncancerous human embryonic kidney cell Hek 293.
authors.affiliation: Department of Chemistry and the Center for Innovation in Chemistry (PERCH-CIC), Faculty of Science, Mahidol University, Bangkok, Thailand.
authors.forename: Waraporn
authors.initials: W
authors.lastname: Chanakul
authors.forename: Patoomratana
authors.initials: P
authors.lastname: Tuchinda
authors.forename: Natthinee
authors.initials: N
authors.lastname: Anantachoke
authors.forename: Manat
authors.initials: M
authors.lastname: Pohmakotr
authors.forename: Pawinee
authors.initials: P
authors.lastname: Piyachaturawat
authors.forename: Surawat
authors.initials: S
authors.lastname: Jariyawat
authors.forename: Kanoknetr
authors.initials: K
authors.lastname: Suksen
authors.forename: Tharworn
authors.initials: T
authors.lastname: Jaipetch
authors.forename: Narong
authors.initials: N
authors.lastname: Nuntasaen
authors.forename: Vichai
authors.initials: V
authors.lastname: Reutrakul
mesh_terms: D000470:Alkaloids / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D029061:Annonaceae / Q000737:chemistry*; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D034341:Aristolochic Acids / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D002460:Cell Line; D045744:Cell Line, Tumor; D006801:Humans; D051379:Mice; D015394:Molecular Structure; D009369:Neoplasms / Q000188:drug therapy*; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D018515:Plant Leaves; D018547:Plant Stems; D051381:Rats
chemical_list: C570370:3,5-dihydroxy-2,4-dimethoxyaristolactam; D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D034341:Aristolochic Acids; D010936:Plant Extracts