title: Gene expression profiles of yeast Saccharomyces cerevisiae sod1 caused by patulin toxicity and evaluation of recovery potential of ascorbic acid.
abstract: Patulin (PAT) is a fungal secondary metabolite and exhibits various toxicities including DNA damage and oxidative stress. These toxicities are eased by ascorbic acid (AsA). Although a number of studies regarding the mitigating effect of AsA against PAT toxicity have been reported, a comprehensive study about gene expressions is currently underway. Here, we carried out a detailed evaluation of PAT toxicity by co-incubation with AsA using the superoxide dismutase (SOD) mutant. DNA microarray results extracted the alterations in iron transporter and Fe/S cluster assembly genes; some of the genes that constitute the cellular iron transporter systems remained dysfunctional even in the presence of AsA. Meanwhile, AsA treatment reduced the alterations of G1/S phase cell cycle regulation genes. These results suggest that oxidative stress-derived DNA damage still exists, although AsA treatment effectively reduces PAT toxicity. This implies that a combined condition is required for complete blockade of PAT toxicity.
authors.affiliation: Applied Microbiology Division, National Food Research Institute, Tsukuba, Ibaraki 305-8642, Japan.
authors.forename: Tadahiro
authors.initials: T
authors.lastname: Suzuki
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Iwahashi
mesh_terms: D001205:Ascorbic Acid / Q000494:pharmacology*; D004249:DNA Damage / Q000187:drug effects; D015870:Gene Expression / Q000187:drug effects; D020869:Gene Expression Profiling; D009153:Mutagens / Q000633:toxicity*; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D018384:Oxidative Stress / Q000187:drug effects; D010365:Patulin / Q000037:antagonists & inhibitors / Q000633:toxicity*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D013482:Superoxide Dismutase / Q000235:genetics*; D000072105:Superoxide Dismutase-1
chemical_list: D009153:Mutagens; D010365:Patulin; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; D001205:Ascorbic Acid