title: Diverse LEA (late embryogenesis abundant) and LEA-like genes and their responses to hypersaline stress in post-diapause embryonic development of Artemia franciscana.
abstract: From post-diapause cysts of Artemia franciscana, we defined fourteen LEA (late embryogenesis abundant) and LEA-like genes, including four novel members (Afrlea1-5, Afrlea3-5, Afrlea3-like1 and Afrlea3-like2), which were classified into four groups: G1, G3, G3-like (LEA group3-like), and SMP-like (seed-maturation-protein-like), based on their conserved and diversified sequence motifs and amino acid compositions among bacteria, plants, and animals. We also validated six representative genes based on quantitative RT-PCR, including three LEA and two LEA3-like genes that are down-regulated when dehydrated cysts hatch to desiccation-intolerant larvae as well as one SMP-like gene that is slightly up-regulated. We further tested their responses to hypersaline stress for four representatives-one from each group-and found that the expression of Afrlea1-5 and Afrlea3-2 were inducible but not Afrlea3-like1 and Afrsmp-like. This result suggested that the LEA and LEA-like genes may play different roles in resistance to hypersaline stress.
authors.affiliation: CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, China. wugang@big.ac.cn
authors.forename: Gang
authors.initials: G
authors.lastname: Wu
authors.forename: Hangxiao
authors.initials: H
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Sun
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
authors.forename: Xiaomeng
authors.initials: X
authors.lastname: Ge
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Chen
authors.forename: Jun
authors.initials: J
authors.lastname: Yu
authors.forename: Weiwei
authors.initials: W
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001156:Artemia / Q000196:embryology* / Q000235:genetics* / Q000502:physiology; D005786:Gene Expression Regulation; D008018:Life Cycle Stages / Q000502:physiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D054712:Salinity; D016415:Sequence Alignment; D013312:Stress, Physiological
chemical_list: D010940:Plant Proteins; D011506:Proteins; C066246:late embryogenesis abundant protein, plant