title: Age-associated development of inflammation in Wistar rats: Effects of caloric restriction.
abstract: BACKGROUND
  Insulin resistance and type 2 Diabetes have been associated to a low grade of inflammation and their prevalence increase with ageing.
  
  
  OBJECTIVE
  To analyse the development of inflammation in adipose tissue, liver, muscle and hypothalamus during ageing and the effects of caloric restriction.
  
  
  METHODS
  We have analysed the expression of inflammatory cytokines (TNFα, IL1-β, IL-12B and IL-6), proteins involved in macrophage recruitment (MCP-1, CCR2), TLR4 and macrophage markers (CD11c, CD11b and arginase1). Immunohistochemistry of macrophages has also been performed.
  
  
  RESULTS
  All studied tissues present signs of inflammation during ageing, but with different pattern and intensity. Caloric restriction decreases the expression of most of inflammatory markers.
  
  
  CONCLUSIONS
  These data indicate a role of adiposity in the development of inflammation and insulin resistance during ageing. Dietetic intervention could be a useful tool to ameliorate the development of inflammation and insulin resistance associated with ageing.
authors.affiliation: Facultad de Ciencias de la Salud, Universidad Rey Juan Carlos, Alcorcón, 28922, Madrid, Spain.
authors.forename: Daniel
authors.initials: D
authors.lastname: Horrillo
authors.forename: Johanna
authors.initials: J
authors.lastname: Sierra
authors.forename: Carmen
authors.initials: C
authors.lastname: Arribas
authors.forename: Miriam
authors.initials: M
authors.lastname: García-San Frutos
authors.forename: José M
authors.initials: JM
authors.lastname: Carrascosa
authors.forename: Nuria
authors.initials: N
authors.lastname: Lauzurica
authors.forename: Teresa
authors.initials: T
authors.lastname: Fernández-Agulló
authors.forename: Manuel
authors.initials: M
authors.lastname: Ros
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism / Q000473:pathology; D000375:Aging / Q000502:physiology*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D031204:Caloric Restriction; D016207:Cytokines / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism; D006801:Humans; D007031:Hypothalamus / Q000378:metabolism / Q000473:pathology; D007249:Inflammation / Q000378:metabolism*; D007333:Insulin Resistance / Q000502:physiology; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008264:Macrophages / Q000378:metabolism; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism / Q000473:pathology; D018384:Oxidative Stress; D012313:RNA / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
chemical_list: D015415:Biomarkers; D016207:Cytokines; D012313:RNA