title: Proteomic analysis of phosphotyrosyl proteins in human embryonic stem cell-derived neural stem cells.
abstract: Phosphorylation can reveal essential cell functions, such as cell differentiation, signal transduction, metabolic maintenance and cell division. The aim of this study was to investigate phosphorylated protein expression changes during neuronal lineage differentiation from hESCs. To measure the phosphorylated protein expression change during neuronal differentiation, we performed a comparative phosphoproteome analysis using 2-DE after MALDI-TOF MS and an MS/MS protein identification method, making a comparison between neural lineage differentiating cells and normal embryoid bodies (EBs) differentiated from human embryonic stem cells (hESCs) and profiling constituent phosphorylated proteins. Of 36 differentially expressed protein spots, 12 spots were shown to be up-regulated in differentiating neural cells. Specifically, the 7 up-regulated proteins of the 12 have potential roles in neuronal differentiation or neuronal damage recovery, including ACTB, heterogeneous nuclear ribonucleoprotein A2B1 (hnRNP A2B1), heterogeneous nuclear ribonucleoprotein L (hnRNP L), SET, chaperonin-containing TCP-1, vimentin and voltage-dependent anion channel protein 1 (VDAC1). These proteins are discussed further below.
authors.affiliation: CHA Bio & Diostech Co., Ltd., 606-16 Yeoksam 1 dong, Gangnam gu, Seoul 135-907, Republic of Korea.
authors.forename: Jumi
authors.initials: J
authors.lastname: Kim
authors.forename: Ji-Su
authors.initials: JS
authors.lastname: Kim
authors.forename: Hye Eun
authors.initials: HE
authors.lastname: Kim
authors.forename: Young-Joo
authors.initials: YJ
authors.lastname: Jeon
authors.forename: Dong-Wook
authors.initials: DW
authors.lastname: Kim
authors.forename: Yunjo
authors.initials: Y
authors.lastname: Soh
authors.forename: Kang Seok
authors.initials: KS
authors.lastname: Seo
authors.forename: Hak Kyo
authors.initials: HK
authors.lastname: Lee
authors.forename: Nag-Jin
authors.initials: NJ
authors.lastname: Choi
authors.forename: Hyung Min
authors.initials: HM
authors.lastname: Chung
authors.forename: Dong-Seok
authors.initials: DS
authors.lastname: Lee
authors.forename: Jung-Il
authors.initials: JI
authors.lastname: Chae
mesh_terms: D002454:Cell Differentiation; D002460:Cell Line; D058732:Embryoid Bodies / Q000378:metabolism; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism; D006801:Humans; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D058953:Neural Stem Cells / Q000378:metabolism*; D010766:Phosphorylation; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D015854:Up-Regulation
chemical_list: D009419:Nerve Tissue Proteins