title: Determination of the chemical composition and in vitro antioxidant activities of essential oil and methanol extracts of Echinophora platyloba DC.
abstract: This study was designed to examine the chemical composition and in vitro antioxidant activity of essential oil and methanol extracts of Echinophora platyloba from Iran. Gas chromatography (GC) and GC/MS (mass spectrometry; MS) analysis of the essential oil resulted in the identification of 29 compounds, which comprised 97.4% of the oil. The main constituents were found to be: (Z)-β-ocimene (26.7%), Δ-3-carene (16.2%) and limonene (6.6%). Antioxidant activities of the essential oil and the methanolic extracts from E. platyloba were evaluated using three different test systems, namely 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, β-carotene-linoleic acid bleaching and reducing power assays. In the DPPH system, the highest radical-scavenging activity was shown by the polar sub-fraction of methanol extract (71.2 ± 1.11 µg mL(-1)). Also in the second case, the relative inhibition capacity (%) of the essential oil (68.0 ± 1.14%) was found to be the stronger one. In addition, the amounts of total phenol components in the polar sub-fractions of methanolic extract (67.5 ± 0.48 µg mg(-1)), nonpolar sub-fractions of methanol extract (35.3 ± 0.12) and the oil (83.3 ± 0.24 µg mg(-1)) were determined.
authors.affiliation: Department of Chemistry, Razi University, Kermanshah, Iran. mbgholivand@yahoo.com
authors.forename: M B
authors.initials: MB
authors.lastname: Gholivand
authors.forename: M
authors.initials: M
authors.lastname: Rahimi-Nasrabadi
authors.forename: E
authors.initials: E
authors.lastname: Mehraban
authors.forename: M
authors.initials: M
authors.lastname: Niasari
authors.forename: H
authors.initials: H
authors.lastname: Batooli
mesh_terms: D000080462:Acyclic Monoterpenes; D000475:Alkenes / Q000032:analysis; D000975:Antioxidants / Q000032:analysis*; D019661:Apiaceae / Q000737:chemistry*; D000081008:Bicyclic Monoterpenes; D001713:Biphenyl Compounds; D053138:Cyclohexenes / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D066298:In Vitro Techniques; D007492:Iran; D000077222:Limonene; D000432:Methanol; D039821:Monoterpenes / Q000032:analysis; D009822:Oils, Volatile / Q000737:chemistry*; D010636:Phenols / Q000032:analysis; D010851:Picrates; D010936:Plant Extracts / Q000737:chemistry*; D013729:Terpenes / Q000032:analysis
chemical_list: D000080462:Acyclic Monoterpenes; D000475:Alkenes; D000975:Antioxidants; D000081008:Bicyclic Monoterpenes; D001713:Biphenyl Compounds; D053138:Cyclohexenes; D039821:Monoterpenes; D009822:Oils, Volatile; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D013729:Terpenes; C443996:beta-ocimene; D000077222:Limonene; C004931:1,1-diphenyl-2-picrylhydrazyl; C030218:3-carene; D000432:Methanol