title: The earliest intrathymic precursors of CD8α(+) thymic dendritic cells correspond to myeloid-type double-negative 1c cells.
abstract: The dendritic cells (DCs) present in lymphoid and non-lymphoid organs are generated from progenitors with myeloid-restricted potential. However, in the thymus a major subset of DCs expressing CD8α and langerin (CD207) appears to stand apart from all other DCs in that it is thought to derive from progenitors with lymphoid potential. Using mice expressing a fluorescent reporter and a diphtheria toxin receptor under the control of the cd207 gene, we demonstrated that CD207(+) CD8α(+) thymic DCs do not share a common origin with T cells but originate from intrathymic precursors that express markers that are normally present on all (CD11c(+) and MHCII molecules) or on some (CD207, CD135, CD8α, CX3CR1) DC subsets. Those intrathymic myeloid-type precursors correspond to CD44(+) CD25(-) double-negative 1c (DN1c) cells and are continuously renewed from bone marrow-derived canonical DC precursors. In conclusion, our results demonstrate that the earliest intrathymic precursors of CD8α(+) thymic DCs correspond to myeloid-type DN1c cells and support the view that under physiological conditions myeloid-restricted progenitors generate the whole constellation of DCs present in the body including the thymus.
authors.affiliation: Centre d'Immunologie de Marseille-Luminy, Université de la Méditerranée, Marseille, France.
authors.forename: Hervé
authors.initials: H
authors.lastname: Luche
authors.forename: Laurence
authors.initials: L
authors.lastname: Ardouin
authors.forename: Pearline
authors.initials: P
authors.lastname: Teo
authors.forename: Peter
authors.initials: P
authors.lastname: See
authors.forename: Sandrine
authors.initials: S
authors.lastname: Henri
authors.forename: Miriam
authors.initials: M
authors.lastname: Merad
authors.forename: Florent
authors.initials: F
authors.lastname: Ginhoux
authors.forename: Bernard
authors.initials: B
authors.lastname: Malissen
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D016827:CD8 Antigens / Q000276:immunology* / Q000378:metabolism; D002454:Cell Differentiation / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D018960:Hyaluronan Receptors / Q000276:immunology / Q000378:metabolism; D050835:Interferon Regulatory Factors / Q000276:immunology / Q000378:metabolism; D053645:Interleukin-2 Receptor alpha Subunit / Q000276:immunology / Q000378:metabolism; D037181:Lectins, C-Type / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D008297:Male; D037241:Mannose-Binding Lectins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D018448:Models, Immunological; D022423:Myeloid Cells / Q000276:immunology* / Q000378:metabolism; D013154:Spleen / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D013950:Thymus Gland / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D013997:Time Factors; D000099185:Interferon Regulatory Factor-8
chemical_list: D000954:Antigens, Surface; D016827:CD8 Antigens; D049452:Green Fluorescent Proteins; D018960:Hyaluronan Receptors; D050835:Interferon Regulatory Factors; D053645:Interleukin-2 Receptor alpha Subunit; D037181:Lectins, C-Type; D037241:Mannose-Binding Lectins; D000099185:Interferon Regulatory Factor-8; C431698:CD8alpha antigen; C498497:Cd207 protein, mouse; C485184:enhanced green fluorescent protein