title: Role played by exosporium glycoproteins in the surface properties of Bacillus cereus spores and in their adhesion to stainless steel.
abstract: Bacillus cereus spores are surrounded by a loose-fitting layer called the exosporium, whose distal part is mainly formed from glycoproteins. The role played by the exosporium glycoproteins of B. cereus ATCC 14579 (BclA and ExsH) was investigated by considering hydrophobicity and charge, as well as the properties of spore adhesion to stainless steel. The absence of BclA increased both the isoelectric point (IEP) and hydrophobicity of whole spores while simultaneously reducing the interaction between spores and stainless steel. However, neither the hydrophobicity nor the charge associated with BclA could explain the differences in the adhesion properties. Conversely, ExsH, another exosporium glycoprotein, did not play a significant role in spore surface properties. The monosaccharide analysis of B. cereus ATCC 14579 showed different glycosylation patterns on ExsH and BclA. Moreover, two specific glycosyl residues, namely, 2-O-methyl-rhamnose (2-Me-Rha) and 2,4-O-methyl-rhamnose (2,4-Me-Rha), were attached to BclA, in addition to the glycosyl residues already reported in B. anthracis.
authors.affiliation: INRA, UR638, F-59651 Villeneuve d'Ascq, France.
authors.forename: Yannick
authors.initials: Y
authors.lastname: Lequette
authors.forename: Estelle
authors.initials: E
authors.lastname: Garénaux
authors.forename: Grégoire
authors.initials: G
authors.lastname: Tauveron
authors.forename: Sylvain
authors.initials: S
authors.lastname: Dumez
authors.forename: Stéphane
authors.initials: S
authors.lastname: Perchat
authors.forename: Christian
authors.initials: C
authors.lastname: Slomianny
authors.forename: Didier
authors.initials: D
authors.lastname: Lereclus
authors.forename: Yann
authors.initials: Y
authors.lastname: Guérardel
authors.forename: Christine
authors.initials: C
authors.lastname: Faille
mesh_terms: D001409:Bacillus cereus / Q000737:chemistry* / Q000166:cytology* / Q000378:metabolism; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D002473:Cell Wall / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D008562:Membrane Glycoproteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008766:Methylmannosides / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D013171:Spores, Bacterial / Q000737:chemistry; D013193:Stainless Steel; D013499:Surface Properties
chemical_list: D001426:Bacterial Proteins; D008562:Membrane Glycoproteins; D008766:Methylmannosides; D013193:Stainless Steel; C035898:2-O-methylrhamnose