title: Genetic diversity analysis of abiotic stress response gene TaSnRK2.7-A in common wheat.
abstract: Sucrose non-fermenting1-related protein kinase 2 (SnRK2) plays a key role in plant stress signaling transduction pathways. In this study, one copy of TaSnRK2.7, a SnRK2 member of common wheat, was isolated and characterized for nucleotide diversity among 45 wheat accessions with different stress-response features. Most of the accessions were elite wheat cultivars, which had been subject to population bottlenecks and intensive selection during breeding. Nucleotide and haplotype diversity across the entire TaSnRK2.7-A region was 0.00076 and 0.590, respectively, and diversity in non-coding regions was higher than that in coding regions. Sliding-window analysis showed variable levels of nucleotide variation along the entire TaSnRK2.7-A region; the sixth intron and ninth exon represented variation-enriched regions. As predicted, neutrality tests revealed that population bottlenecks or purifying selection had acted on the TaSnRK2.7-A gene, a relatively conserved gene. Furthermore, strong linkage disequilibrium between SNP loci extends across the entire TaSnRK2.7-A region. These findings demonstrate that the TaSnRK2.7-A genomic region has evolved under extensive selection pressure during crop breeding.
authors.affiliation: The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Hongying
authors.initials: H
authors.lastname: Zhang
authors.forename: Xinguo
authors.initials: X
authors.lastname: Mao
authors.forename: Jianan
authors.initials: J
authors.lastname: Zhang
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Chang
authors.forename: Chengshe
authors.initials: C
authors.lastname: Wang
authors.forename: Ruilian
authors.initials: R
authors.lastname: Jing
mesh_terms: D001483:Base Sequence; D001947:Breeding; D016000:Cluster Analysis; D056426:Genetic Loci / Q000235:genetics; D014644:Genetic Variation; D015810:Linkage Disequilibrium / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D012641:Selection, Genetic; D016415:Sequence Alignment; D013312:Stress, Physiological / Q000235:genetics*; D014908:Triticum / Q000201:enzymology* / Q000235:genetics*
chemical_list: D010940:Plant Proteins