title: The enhancement of mature vessel formation and cardiac function in infarcted hearts using dual growth factor delivery with self-assembling peptides.
abstract: For successful treatment of myocardial infarction (MI), it is important to prevent cardiac fibrosis and maintain cardiac function by protecting cardiomyocytes and inducing angiogenesis. To establish functional and stable vessels, various growth factors, ones stimulating both endothelial cells (EC) and vascular smooth muscle cells (VSMC), are required. Self-assembling peptides form fibers (<10 nm) and provide 3-dimensional microenvironments that can recruit EC and VSMC to promote vascularization and long-term delivery of growth factors. Here we demonstrate myocardial protection of infarcted heart using dual growth factor delivery with self-assembling peptides. After coronary artery ligation in rats, growth factors (PDGF-BB and FGF-2) with self-assembling peptides were injected. There were 6 rats in each group. Hearts were harvested at 4 and 8 weeks for functional and histological analysis. Infarct size and cardiomyocyte apoptosis in dual growth factors along with self-assembling peptides group were dramatically reduced compared to sham. The capillary and arterial density of this group recovered with angiogenic synergism and cardiac functions had almost recovered. In conclusion, dual growth factors along with self-assembling peptides lead to myocardial protection, stable vessel formation, and improvement in cardiac function.
authors.affiliation: Biomaterials Research Center, Korea Institute of Science and Technology, Seoul 136-791, Republic of Korea.
authors.forename: Ji Hyun
authors.initials: JH
authors.lastname: Kim
authors.forename: Youngmee
authors.initials: Y
authors.lastname: Jung
authors.forename: Sang-Heon
authors.initials: SH
authors.lastname: Kim
authors.forename: Kyung
authors.initials: K
authors.lastname: Sun
authors.forename: Jaesoon
authors.initials: J
authors.lastname: Choi
authors.forename: Hee Chan
authors.initials: HC
authors.lastname: Kim
authors.forename: Yongdoo
authors.initials: Y
authors.lastname: Park
authors.forename: Soo Hyun
authors.initials: SH
authors.lastname: Kim
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D000077214:Becaplermin; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage / Q000737:chemistry* / Q000627:therapeutic use*; D020287:In Situ Nick-End Labeling; D008297:Male; D009203:Myocardial Infarction / Q000188:drug therapy*; D032383:Myocytes, Cardiac / Q000166:cytology / Q000187:drug effects; D018919:Neovascularization, Physiologic / Q000187:drug effects; D010455:Peptides / Q000008:administration & dosage* / Q000737:chemistry*; D010982:Platelet-Derived Growth Factor / Q000008:administration & dosage / Q000737:chemistry / Q000627:therapeutic use*; D020574:Proto-Oncogene Proteins c-sis; D051381:Rats; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D010455:Peptides; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D016222:Fibroblast Growth Factor 2; D000077214:Becaplermin