title: Enzymatic synthesis and properties of uridine-5'-O-(2-thiodiphospho)-N-acetylglucosamine.
abstract: This paper describes an enzymatic approach to obtain a thio-containing UDP-GlcNAc analog. We use an assay based on capture of the carbohydrate and analysis by mass spectrometry to quantitatively characterize the activity of this unnatural sugar donor in a LgtA-mediated glycosylation reaction.
authors.affiliation: Departments of Chemistry and Biochemistry, The Ohio State University, 484 W. 12th Ave., Columbus, OH 43210, USA.
authors.forename: Li
authors.initials: L
authors.lastname: Cai
authors.forename: Lan
authors.initials: L
authors.lastname: Ban
authors.forename: Wanyi
authors.initials: W
authors.lastname: Guan
authors.forename: Milan
authors.initials: M
authors.lastname: Mrksich
authors.forename: Peng George
authors.initials: PG
authors.lastname: Wang
mesh_terms: D002412:Cations / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D006031:Glycosylation; D016695:Glycosyltransferases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006046:Gold / Q000737:chemistry; D007700:Kinetics; D007785:Lactose / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D009345:Neisseria meningitidis / Q000737:chemistry / Q000201:enzymology*; D015202:Protein Engineering / Q000379:methods*; D013379:Substrate Specificity; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D014537:Uridine Diphosphate N-Acetylglucosamine / Q000096:biosynthesis / Q000737:chemistry
chemical_list: D002412:Cations; D013438:Sulfhydryl Compounds; D014537:Uridine Diphosphate N-Acetylglucosamine; D006046:Gold; D004492:Edetic Acid; D016695:Glycosyltransferases; D007785:Lactose