title: MyD88-dependent Toll-like receptor signaling is required for murine macrophages response to IS2.
abstract: IS2, a soluble β-glucan isolated from the cell wall of mutated Saccharomyces cerevisiae (S. cerevisiae) enhances the immune response compared to the wild type (WT) β-glucan. In the present investigation we report that Toll-like receptor (TLR)/MyD88 signaling pathway was responsible in IS2 β-glucan-mediated cellular response in RAW264.7 murine macrophages. Data revealed that IS2 β-glucan significantly up-regulated the TLR2/TLR4 expression. Moreover, TLR2/TLR4 responds to IS2 resulting in murine macrophage activation. In addition, the IS2 signal led to cytokine secretions of IL-6 and TNF-α. In the case of thioglycolate-elicited peritoneal macrophages from MyD88-deficient mice, the decrease in cytokines was observed. Further the mitogen-activated protein kinases (MAPKs) phosphorylation was evident and degradation of IκB-α was increased after stimulation with IS2 β-glucan. Further examination with MyD88-deficient mice revealed that the MyD88 pathway might play an important role for IS2 β-glucan-mediated activation of macrophages.
authors.affiliation: Department of Biotechnology, College of Biomedical & Health Science, Konkuk University, Chungju 380-701, Korea.
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.forename: Wan-Jae
authors.initials: WJ
authors.lastname: Kim
authors.forename: Jun
authors.initials: J
authors.lastname: Jiang
authors.forename: Seung-Hwan
authors.initials: SH
authors.lastname: Lee
authors.forename: Hyung-Sun
authors.initials: HS
authors.lastname: Youn
authors.forename: Eun-Yi
authors.initials: EY
authors.lastname: Moon
authors.forename: Tack-Joong
authors.initials: TJ
authors.lastname: Kim
authors.forename: Sang-Kyu
authors.initials: SK
authors.lastname: Ye
authors.forename: Ji-Hwan
authors.initials: JH
authors.lastname: Ryu
authors.forename: Tae-Bong
authors.initials: TB
authors.lastname: Kang
authors.forename: Sushruta
authors.initials: S
authors.lastname: Koppula
authors.forename: Pyo-Jam
authors.initials: PJ
authors.lastname: Park
authors.forename: Dong-Kug
authors.initials: DK
authors.lastname: Choi
authors.forename: Kwang-Ho
authors.initials: KH
authors.lastname: Lee
mesh_terms: D000818:Animals; D002460:Cell Line; D005786:Gene Expression Regulation / Q000187:drug effects / Q000276:immunology; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D008262:Macrophage Activation / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D051379:Mice; D018345:Mice, Knockout; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D053594:Myeloid Differentiation Factor 88 / Q000276:immunology / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000276:immunology*; D015398:Signal Transduction / Q000187:drug effects / Q000276:immunology; D051195:Toll-Like Receptor 2 / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D051197:Toll-Like Receptor 4 / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism; D047071:beta-Glucans / Q000008:administration & dosage* / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: C569927:IS2 beta-glucan; D015850:Interleukin-6; D053594:Myeloid Differentiation Factor 88; C493486:Tlr2 protein, mouse; C493487:Tlr4 protein, mouse; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; D014409:Tumor Necrosis Factor-alpha; D047071:beta-Glucans; D020928:Mitogen-Activated Protein Kinases