title: Planococcus plakortidis sp. nov., isolated from the marine sponge Plakortis simplex (Schulze).
abstract: A novel coccoid-shaped strain, AS/ASP6 (II)T, was isolated from a sample taken from Plakortis simplex (Schulze), a marine sponge, collected at a depth of 30 m from the Bay of Bengal. This strain was identified by using a polyphasic taxonomic approach. The results of 16S rRNA gene sequence analysis showed that strain AS/ASP6 (II)T should be assigned to the genus Planococcus. Chemotaxonomic data (A4α-type peptidoglycan; MK-6, MK-7 and MK-8 menaquinones; mainly branched cellular fatty acids; and phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol as cellular phospholipids) supported taxonomic placement in the genus Planococcus. Phylogenetic analysis of the 16S rRNA gene sequence showed that strain AS/ASP6 (II)T belonged to the genus Planococcus and was closely related to the type strains of Planococcus maritimus (99.1 %) followed by Planococcus rifietoensis (98.6 %), Planococcus maitriensis (98.5 %), Planococcus citreus (98.3 %), Planococcus salinarum (98.1 %), Planococcus columbae (97.9 %), Planococcus donghaensis (97.8 %) and Planococcus antarcticus (97.7 %); DNA-DNA hybridization values obtained were well below the threshold that is required for the proposal of a novel species. The G+C content of the genomic DNA was 51.0  mol%. The phenotypic and genotypic data showed that strain AS/ASP6 (II)T merits recognition as a representative of a novel species of the genus Planococcus, for which the name Planococcus plakortidis sp. nov. is proposed; the type strain is AS/ASP6 (II)T (=MTCC 8491T=DSM 23997T).
authors.affiliation: MTCC - Microbial Type Culture Collection and Gene Bank, CSIR - Institute of Microbial Technology, Chandigarh 160 036, India.
authors.forename: Ishwinder
authors.initials: I
authors.lastname: Kaur
authors.affiliation: Department of Natural Products, CSIR - Institute of Minerals and Materials Technology, Bhubaneshwar 751 013, India.
authors.forename: A P
authors.initials: AP
authors.lastname: Das
authors.affiliation: Department of Natural Products, CSIR - Institute of Minerals and Materials Technology, Bhubaneshwar 751 013, India.
authors.forename: M
authors.initials: M
authors.lastname: Acharya
authors.affiliation: DSMZ - German Collection of Microorganisms and Cell Cultures, 38124, Braunschweig, Germany.
authors.forename: H-P
authors.initials: HP
authors.lastname: Klenk
authors.affiliation: Department of Natural Products, CSIR - Institute of Minerals and Materials Technology, Bhubaneshwar 751 013, India.
authors.forename: A
authors.initials: A
authors.lastname: Sree
authors.affiliation: MTCC - Microbial Type Culture Collection and Gene Bank, CSIR - Institute of Microbial Technology, Chandigarh 160 036, India.
authors.forename: S
authors.initials: S
authors.lastname: Mayilraj
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D061006:Bays; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D007194:India; D019156:Indian Ocean; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D047229:Plakortis / Q000382:microbiology*; D058642:Planococcus Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2