title: Complex of digestive proteinases of Galleria mellonella Caterpillars: composition, properties, and limited proteolysis of Bacillus thuringiensis endotoxins.
abstract: The complex of digestive proteinases in caterpillars of the greater wax moth Galleria mellonella was studied. Using chromogenic substrates and inhibitor analysis, it was found that serine proteinases play a key role in this complex. Three anionic and two cationic forms of trypsin and one anionic and one cationic form of chymotrypsin were identified by zymography in the midgut extract of G. mellonella. The most active trypsin was purified to electrophoretic homogeneity, and its N-terminal amino acid sequence was shown to be identical to that of mature trypsin from Plodia interpunctella. Midgut extract from G. mellonella was capable of processing Cry-proteins from Bacillus thuringiensis ssp. galleriae. Enzymes with tryptic and chymotryptic activities participate in this process, and activation of protoxin Cry9A is not the rate-limiting stage in the toxic action of this protein on the greater wax moth.
authors.affiliation: Scientific Research Institute for Genetics and Selection of Industrial Microorganisms, Moscow, Russia.
authors.forename: N V
authors.initials: NV
authors.lastname: Bulushova
authors.forename: E N
authors.initials: EN
authors.lastname: Elpidina
authors.forename: D P
authors.initials: DP
authors.lastname: Zhuzhikov
authors.forename: L I
authors.initials: LI
authors.lastname: Lyutikova
authors.forename: F
authors.initials: F
authors.lastname: Ortego
authors.forename: N E
authors.initials: NE
authors.lastname: Kirillova
authors.forename: I A
authors.initials: IA
authors.lastname: Zalunin
authors.forename: G G
authors.initials: GG
authors.lastname: Chestukhina
mesh_terms: D000818:Animals; D001413:Bacillus thuringiensis / Q000737:chemistry / Q000378:metabolism; D055162:Biocatalysis; D004064:Digestive System / Q000737:chemistry / Q000201:enzymology; D004731:Endotoxins / Q000737:chemistry*; D019476:Insect Proteins / Q000737:chemistry* / Q000302:isolation & purification; D009036:Moths / Q000737:chemistry / Q000201:enzymology*; D010447:Peptide Hydrolases / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D004731:Endotoxins; D019476:Insect Proteins; D010447:Peptide Hydrolases