title: [The effect of DNA methyltransferase 1 low expression on the global genome DNA methylation status of 16HBE cell].
abstract: OBJECTIVE
  To construct DNA methyltransferase 1 (DNMT1) low expression 16HBE cell line and observe the variation of cell cycle and global genomic DNA methylation.
  
  
  METHODS
  The method of Lenti-virus induced RNA interference was applied to introduce four different shRNA fragment into 16HBE cells. Flow cytometry and 5-mC immunofluorescence methods were used to observe the cell cycle and global DNA methylation status of DNMT1 low expression 16HBE cells.
  
  
  RESULTS
  The DNMT1 protein relative expression level of 16HBE-shDNMT1-4 cell line was down regulated about 44% (P < 0.05) compared with the control. No obvious differences of cell cycle and global genome DNA methylation status were observed between the 16HBE and 16HBE-shDNMT1.
  
  
  CONCLUSIONS
  The DNMT1 gene low expression cell is successfully constructed, and there are no obvious changes happened on the cell cycle and global genomic DNA methylation.
authors.affiliation: Toxicology Laboratory, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China.
authors.forename: Lin-qing
authors.initials: LQ
authors.lastname: Yang
authors.forename: Qing-cheng
authors.initials: QC
authors.lastname: Liu
authors.forename: Chun-mei
authors.initials: CM
authors.lastname: Gong
authors.forename: Gong-hua
authors.initials: GH
authors.lastname: Tao
authors.forename: Jian-jun
authors.initials: JJ
authors.lastname: Liu
authors.forename: Gong-hua
authors.initials: GH
authors.lastname: Hu
authors.forename: Hai-yan
authors.initials: HY
authors.lastname: Huang
authors.forename: Kun-peng
authors.initials: KP
authors.lastname: Wang
authors.forename: Zhi-xiong
authors.initials: ZX
authors.lastname: Zhuang
mesh_terms: D002453:Cell Cycle; D002460:Cell Line; D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000235:genetics / Q000378:metabolism*; D019175:DNA Methylation; D015536:Down-Regulation; D004847:Epithelial Cells / Q000378:metabolism*; D006801:Humans; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics
chemical_list: D034741:RNA, Small Interfering; D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases; C000619380:DNMT1 protein, human