title: MicroRNA-210 as a novel blood biomarker in acute cerebral ischemia.
abstract: MicroRNA-210 (miR-210), a master and pleiotropic hypoxia-microRNA, plays multiple roles in brain ischemia. However, miR-210 expression and its function in humans have not been explored. The aim of our study is to evaluate the correlation of blood miR-210 with clinical findings in acute ischemic stroke. Blood samples were obtained from stroke patients (n=112) and healthy controls (n= 60). MiR-210 was measured at within 3, 7 and 14 days after stroke using a quantitative PCR technique. Stroke severity and clinical outcome were evaluated by NIHSS and modified Rankin Score. Both blood and brain miR-210 in ischemic mice was examined and the correlation was investigated. Compared to healthy controls, blood miRNA-210 was significantly decreased in stroke patients (0.93 vs. 1.36; P=0.001), especially at 7 days (0.56 vs. 1.36; P=0.001) and 14 days of stroke onset (0.50 vs. 1.36; P=0.001). The cut off point of miR-210 in diagnosis was 0.505 with 88.3 per cent sensitivity. MiR-210 level in stroke patients with good outcome was significantly higher than patients with poor outcome (1.2 vs. 0.44; P=0.012). The correlation between blood and brain miR-210 in ischemic mice was positive (R2=0.57, P=0.001). Blood miR-210 is a novel sensitive biomarker for clinical diagnosis and prognosis in acute cerebral ischemia.
authors.affiliation: Department of Neurology, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Zeng
authors.forename: Jianrong
authors.initials: J
authors.lastname: Liu
authors.forename: Yongting
authors.initials: Y
authors.lastname: Wang
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.forename: Suiqing
authors.initials: S
authors.lastname: Weng
authors.forename: Yaohui
authors.initials: Y
authors.lastname: Tang
authors.forename: Chaobo
authors.initials: C
authors.lastname: Zheng
authors.forename: Qi
authors.initials: Q
authors.lastname: Cheng
authors.forename: Shengdi
authors.initials: S
authors.lastname: Chen
authors.forename: Guo-Yuan
authors.initials: GY
authors.lastname: Yang
mesh_terms: D000208:Acute Disease; D000368:Aged; D000818:Animals; D015415:Biomarkers / Q000097:blood*; D002545:Brain Ischemia / Q000097:blood*; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D016133:Polymerase Chain Reaction
chemical_list: D015415:Biomarkers; C543800:MIRN210 microRNA, human; D035683:MicroRNAs