title: [Pilot-scale production of recombinant plasmid pUDK-HGF].
abstract: pUDK-HGF, the recombinant plasmid DNA encoding human hepatocyte growth factor (HGF), can treat ischaemic disease. A great quantity of pharmaceutical pUDK-HGF is needed. A pilot-scale production process of pUDK-HGF was established based on a new chromatographic media (plasmidselect), including fermentation, cell harvesting, alkaline lysis, ultrafiltration, RNA removing and buffer exchanging on Sephacryl S-1000, capturing supercoiled plasmid DNA with plasmidselect, and removing the salt with Sepharose 6BFF. The process does not use RNase enzyme and toxic solvents.
authors.affiliation: Beijing Institute of Radiation Medicine, Beijing 100850, China.
authors.forename: Chunsheng
authors.initials: C
authors.lastname: Hu
authors.forename: Yanliang
authors.initials: Y
authors.lastname: Wang
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Lu
authors.forename: Xiaochen
authors.initials: X
authors.lastname: Cheng
authors.forename: Lin
authors.initials: L
authors.lastname: Liu
authors.forename: Tong
authors.initials: T
authors.lastname: Zhang
authors.forename: Qinglin
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D004274:DNA, Recombinant / Q000096:biosynthesis*; D004278:DNA, Superhelical / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005285:Fermentation; D005822:Genetic Vectors / Q000235:genetics; D017228:Hepatocyte Growth Factor / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D010865:Pilot Projects; D010957:Plasmids / Q000302:isolation & purification*
chemical_list: D004274:DNA, Recombinant; D004278:DNA, Superhelical; D017228:Hepatocyte Growth Factor