title: Selection of housekeeping genes for normalization of RT-PCR in hypoxic neural stem cells of rat in vitro.
abstract: Gene expression analysis under various conditions using real-time reverse transcription polymerase chain reaction (RT-PCR) needs reliable control genes. Housekeeping genes are commonly used as the control. However, no validated housekeeping gene is available for study of hypoxic neural stem cell culture. To choose appropriate internal control genes, the expression of eight commonly used housekeeping genes was examined in rat neural stem cell model to find one or more stably expressed genes under hypoxic/ischemic conditions. Two genes, HPRT and RPL13A were identified as the most confidential housekeeping genes in this research by geNorm and NormFinder softwares. As a groundwork, the most stable housekeeping genes for neural stem cells under hypoxic/ischemic conditions are initially investigated and validated in this experiment, which might provide a better understanding for the gene expression study in ischemic and necrotic neural stem cell cultures or in ischemic diseases of the central nervous system (CNS).
authors.affiliation: Institute of Neurobiology, Environment and Genes Related to Diseases, Key Laboratory of Education Ministry, Xi'an Jiaotong University College of Medicine, and Second Affiliated hospital, Xi'an, 710061, Shaanxi, People's Republic of China.
authors.forename: Lu
authors.initials: L
authors.lastname: Yao
authors.forename: Xinlin
authors.initials: X
authors.lastname: Chen
authors.forename: Yingfang
authors.initials: Y
authors.lastname: Tian
authors.forename: Haixia
authors.initials: H
authors.lastname: Lu
authors.forename: Pengbo
authors.initials: P
authors.lastname: Zhang
authors.forename: Qindong
authors.initials: Q
authors.lastname: Shi
authors.forename: Junfeng
authors.initials: J
authors.lastname: Zhang
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D015687:Cell Hypoxia / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D005333:Fetus; D020043:Genes, Essential / Q000235:genetics*; D066298:In Vitro Techniques; D058953:Neural Stem Cells / Q000378:metabolism*; D051381:Rats; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012107:Research Design; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*
chemical_list: D017931:DNA Primers