title: TIEG1-null tenocytes display age-dependent differences in their gene expression, adhesion, spreading and proliferation properties.
abstract: The remodeling of extracellular matrix is a crucial mechanism in tendon development and the proliferation of fibroblasts is a key factor in this process. The purpose of this study was to further elucidate the role of TIEG1 in mediating important tenocyte properties throughout the aging process. Wildtype and TIEG1 knockout tenocytes adhesion, spreading and proliferation were characterized on different substrates (fibronectin, collagen type I, gelatin and laminin) and the expression levels of various genes known to be involved with tendon development were analyzed by RT-PCR. The experiments revealed age-dependent and substrate-dependent properties for both wildtype and TIEG1 knockout tenocytes. Taken together, our results indicate an important role for TIEG1 in regulating tenocytes adhesion, spreading, and proliferation throughout the aging process. Understanding the basic mechanisms of TIEG1 in tenocytes may provide valuable information for treating multiple tendon disorders.
authors.affiliation: Laboratoire de Biomécanique et Bioingénierie UMR CNRS 6600, Université de Technologie de Compiègne, Compiègne, France.
authors.forename: Oualid
authors.initials: O
authors.lastname: Haddad
authors.forename: Laurie
authors.initials: L
authors.lastname: Gumez
authors.forename: John R
authors.initials: JR
authors.lastname: Hawse
authors.forename: Malayannan
authors.initials: M
authors.lastname: Subramaniam
authors.forename: Thomas C
authors.initials: TC
authors.lastname: Spelsberg
authors.forename: Sabine F
authors.initials: SF
authors.lastname: Bensamoun
mesh_terms: D000367:Age Factors; D000818:Animals; D015153:Blotting, Western; D002448:Cell Adhesion; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000502:physiology*; D015870:Gene Expression; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013708:Tendon Injuries; D013710:Tendons / Q000166:cytology* / Q000378:metabolism*; D014157:Transcription Factors / Q000502:physiology*; D014945:Wound Healing; D051741:Kruppel-Like Transcription Factors; D051765:Early Growth Response Transcription Factors
chemical_list: D024042:Collagen Type I; D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; C569958:KLF10 protein, mouse; D051741:Kruppel-Like Transcription Factors; D051765:Early Growth Response Transcription Factors