title: Crystallization and preliminary X-ray crystallographic analysis of eIF5BΔN and the eIF5BΔN-eIF1AΔN complex.
abstract: The binding between two universally conserved translation initiation factors, eIF5B and eIF1A, is important in the initiation step of eukaryotic protein synthesis on the ribosome. Through this interaction, eIF1A assists in recruiting eIF5B to the initiating 40S subunit; eIF5B then encourages the joining of the 60S subunit to form an initiating 80S ribosome. Here, the expression, purification, crystallization and preliminary X-ray analyses of eIF5BΔN and the eIF5BΔN-eIF1AΔN complex from Saccharomyces cerevisiae are reported. The crystal of eIF5BΔN diffracted to 2.45 Å resolution and belonged to space group P4(1)2(1)2, with unit-cell parameters a = b = 130.0, c = 71.7 Å. The asymmetric unit was estimated to contain one molecule. The initial phase was obtained by Se-SAD. The crystal of the eIF5BΔN-eIF1AΔN complex diffracted to 3.3 Å resolution and belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 101.9, b = 120.9, c = 132.8 Å. The asymmetric unit was estimated to contain two complex molecules.
authors.affiliation: Graduate School of Life Sciences, Hokkaido University, Sapporo, Japan.
authors.forename: Aiping
authors.initials: A
authors.lastname: Zheng
authors.forename: Reo
authors.initials: R
authors.lastname: Yamamoto
authors.forename: Masaaki
authors.initials: M
authors.lastname: Sokabe
authors.forename: Isao
authors.initials: I
authors.lastname: Tanaka
authors.forename: Min
authors.initials: M
authors.lastname: Yao
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D015977:Eukaryotic Initiation Factor-1 / Q000737:chemistry* / Q000378:metabolism; D039642:Eukaryotic Initiation Factors / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000378:metabolism
chemical_list: D015977:Eukaryotic Initiation Factor-1; D039642:Eukaryotic Initiation Factors; C451459:eukaryotic initiation factor-5B; C026022:eukaryotic peptide initiation factor-1A