title: Oxalate-degrading capacities of lactic acid bacteria in canine feces.
abstract: In this study, lactic acid bacteria in canine feces were isolated and identified, and their oxalate-degrading capacities were evaluated. The oxalate-degrading capacities were determined for 24 of 47 (51.06%) lactic acid bacteria isolates. Of these, 8 isolates [Leuconostoc mesenteroides (RL75), Lactococcus garvieae (CD2), Lactococcus subsp. lactis (CS21), Enterococcus faecium (CL71 and CL72), and Enterococcus faecalis (CD14, CS62, and CD12)] degraded more than 5% of the oxalate present, while the others degraded less than 5% of the oxalate in vitro. Isolates that degraded more than 5% of the oxalate present were selected for further examination. The oxalate-degrading capacities of individual isolates, a mixture of Enterococcus, a mixture of Lactococcus, and a mixture of the eight isolates were evaluated in media containing different concentrations of glucose (sufficient, insufficient, or no glucose). In comparison with the control medium, all of the individual isolates and mixtures of isolates could degrade oxalate in all three groups (P<0.05). In most cases, the isolates growing in medium with 20 g/L of glucose had higher oxalate-degrading capacities than those growing in medium with 2.5 g/L of glucose or no glucose. The mixture of all isolates showed higher oxalate-degrading capacity than the individual isolates and other mixtures. The oxalate-degrading capacities of the isolates were isolate dependent.
authors.affiliation: Institute of Nutritional and Metabolic Disorders in Domestic Animals and Fowls, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Ren
authors.forename: Cuiling
authors.initials: C
authors.lastname: Pan
authors.forename: Luyan
authors.initials: L
authors.lastname: Jiang
authors.forename: Cong
authors.initials: C
authors.lastname: Wu
authors.forename: Yongwang
authors.initials: Y
authors.lastname: Liu
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Zhong
authors.forename: Linwu
authors.initials: L
authors.lastname: Ran
authors.forename: Fei
authors.initials: F
authors.lastname: Ren
authors.forename: Xingxiang
authors.initials: X
authors.lastname: Chen
authors.forename: Yangguang
authors.initials: Y
authors.lastname: Wang
authors.forename: Yongxing
authors.initials: Y
authors.lastname: Zhu
authors.forename: Kehe
authors.initials: K
authors.lastname: Huang
mesh_terms: D000818:Animals; D004285:Dogs / Q000382:microbiology*; D005243:Feces / Q000382:microbiology*; D005947:Glucose / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism*; D056546:Lactobacillales / Q000145:classification* / Q000302:isolation & purification* / Q000378:metabolism; D010070:Oxalates / Q000032:analysis / Q000378:metabolism*
chemical_list: D010070:Oxalates; D019344:Lactic Acid; D005947:Glucose