title: [Clinic research of the effect of bFGF on human degenerated nucleus pulposus cells].
abstract: OBJECTIVE
  To determine the effect of bFGF on extracellular matrix and gene expression on the human de-generated nucleus pulposus cells.
  
  
  METHODS
  The degenerated intervertebral disc cells were divided into 5 groups. A group: adding 100 pg/L bFGF, B group: adding 200 pg/LbFGF, C group: 500 pg/L bFGF, D group: 1000 pg/L bF-GF, E groups: control group, without interfering factors. The type II collagen and GAG mRNA expression were test, type II collagen content and glycosaminoglycan content of the supernatant were test.
  
  
  RESULTS
  bFGF stimulated de-generated nucleus pulposus cells, collagen II, GAG mRNA, collagen type II and glycosaminoglycan expression of the extracellular matrix were increased significantly on the 7 days compared with the control group (P < 0. 05). type II collagen and GAG mRNA were significantly lower than the control group on 14 days, 21 days (P < 0.05). However,collagen type II and glycosaminoglycan expression of the extracellular matrix on 14 days, 21 days were still higher than normal (P <0.05).
  
  
  CONCLUSIONS
  bFGF increase GAG and type II collagen mRNA expression, increased extracellular matrix type II collagen and GAG expression in short-term.
authors.affiliation: Guangzhou Red Cross Hospital, Guangzhou 510220, China.
authors.forename: Dong-ping
authors.initials: DP
authors.lastname: Ye
authors.forename: Wei-guo
authors.initials: WG
authors.lastname: Liang
authors.forename: Li-bing
authors.initials: LB
authors.lastname: Dai
authors.forename: Yan
authors.initials: Y
authors.lastname: Shen
authors.forename: Hong-hui
authors.initials: HH
authors.lastname: Chen
mesh_terms: D035843:Biomedical Research; D024043:Collagen Type II / Q000187:drug effects / Q000378:metabolism; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism; D005346:Fibroblast Growth Factors / Q000494:pharmacology*; D006025:Glycosaminoglycans / Q000378:metabolism*; D006801:Humans; D007403:Intervertebral Disc / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology*; D055959:Intervertebral Disc Degeneration / Q000188:drug therapy* / Q000473:pathology; D012333:RNA, Messenger / Q000187:drug effects* / Q000378:metabolism
chemical_list: D024043:Collagen Type II; D006025:Glycosaminoglycans; D012333:RNA, Messenger; D005346:Fibroblast Growth Factors