title: Gene expression profile of the regeneration epithelium during axolotl limb regeneration.
abstract: Urodele amphibians are unique among adult vertebrates in their ability to regenerate missing limbs. The process of limb regeneration requires several key tissues including a regeneration-competent wound epidermis called the regeneration epithelium (RE). We used microarray analysis to profile gene expression of the RE in the axolotl, a Mexican salamander. A list of 125 genes and expressed sequence tags (ESTs) showed a ≥1.5-fold expression in the RE than in a wound epidermis covering a lateral cuff wound. A subset of the RE ESTs and genes were further characterized for expression level changes over the time-course of regeneration. This study provides the first large scale identification of specific gene expression in the RE.
authors.affiliation: Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut, USA.
authors.forename: Leah J
authors.initials: LJ
authors.lastname: Campbell
authors.forename: Edna C
authors.initials: EC
authors.lastname: Suárez-Castillo
authors.forename: Humberto
authors.initials: H
authors.lastname: Ortiz-Zuazaga
authors.forename: Dunja
authors.initials: D
authors.lastname: Knapp
authors.forename: Elly M
authors.initials: EM
authors.lastname: Tanaka
authors.forename: Craig M
authors.initials: CM
authors.lastname: Crews
mesh_terms: D000558:Ambystoma mexicanum / Q000235:genetics / Q000502:physiology*; D029845:Amphibian Proteins / Q000235:genetics; D000818:Animals; D004848:Epithelium / Q000378:metabolism / Q000502:physiology*; D020869:Gene Expression Profiling / Q000379:methods*; D017403:In Situ Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D012038:Regeneration / Q000235:genetics / Q000502:physiology*
chemical_list: D029845:Amphibian Proteins