title: FrsA functions as a cofactor-independent decarboxylase to control metabolic flux.
abstract: The interaction between fermentation-respiration switch (FrsA) protein and glucose-specific enzyme IIA(Glc) increases glucose fermentation under oxygen-limited conditions. We show that FrsA converts pyruvate to acetaldehyde and carbon dioxide in a cofactor-independent manner and that its pyruvate decarboxylation activity is enhanced by the dephosphorylated form of IIA(Glc) (d-IIA(Glc)). Crystal structures of FrsA and its complex with d-IIA(Glc) revealed residues required for catalysis as well as the structural basis for the activation by d-IIA(Glc).
authors.affiliation: Department of Environmental Science, Hankuk University of Foreign Studies, Yongin, Republic of Korea.
authors.forename: Kyung-Jo
authors.initials: KJ
authors.lastname: Lee
authors.forename: Chang-Sook
authors.initials: CS
authors.lastname: Jeong
authors.forename: Young Jun
authors.initials: YJ
authors.lastname: An
authors.forename: Hyun-Jung
authors.initials: HJ
authors.lastname: Lee
authors.forename: Soon-Jung
authors.initials: SJ
authors.lastname: Park
authors.forename: Yeong-Jae
authors.initials: YJ
authors.lastname: Seok
authors.forename: Pil
authors.initials: P
authors.lastname: Kim
authors.forename: Jung-Hyun
authors.initials: JH
authors.lastname: Lee
authors.forename: Kyu-Ho
authors.initials: KH
authors.lastname: Lee
authors.forename: Sun-Shin
authors.initials: SS
authors.lastname: Cha
mesh_terms: D000079:Acetaldehyde / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D002245:Carbon Dioxide / Q000378:metabolism; D002262:Carboxy-Lyases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D018360:Crystallography, X-Ray; D003653:Decarboxylation; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005285:Fermentation; D005947:Glucose / Q000378:metabolism*; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008813:Mice, Inbred ICR; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011487:Protein Conformation; D019289:Pyruvic Acid / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D013379:Substrate Specificity; D041261:Vibrio vulnificus / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity; D014774:Virulence
chemical_list: D029968:Escherichia coli Proteins; D011994:Recombinant Proteins; C032242:crr protein, E coli; D002245:Carbon Dioxide; D019289:Pyruvic Acid; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; D002262:Carboxy-Lyases; D000079:Acetaldehyde; D005947:Glucose