title: HLA-DRB1/DQB1 susceptibility for autoimmune polyglandular syndrome type II and III in south of Tunisia.
abstract: OBJECTIVE
  The aim of our study was to investigate the association of HLA-DRB1 and HLA-DQB1 alleles with autoimmune polyglandular syndromes (APS) type II and III in a southern Tunisian population.
  
  
  METHODS
  Sixty-two unrelated patients with APSII (n=20) and APSIII (n=42) and 146 healthy controls were genotyped for HLA class II alleles (DRB1*, DQB1*) by PCR-SSP technique.
  
  
  RESULTS
  An increased frequencies of HLA-DQB1*03:02 (P=0,02; OR=2.98) in APSII patients, HLA-DRB1*03 (P=310(-6); OR=4.28) and HLA-DQB1*02:01 (P=0.04; OR=1.95) in APSIII patients were found compared to healthy controls. Study of the HLA-DRB1*;DQB1* haplotype frequencies showed a higher occurrence of DRB1*04;DQB1*03:02 and DRB1*03;DQB1*02:01 in APSII patients (P=410(-3); OR=3.31 and P=0.03; OR=2.74 respectively) whereas APSIII was only associated with DRB1*03;DQB1*02:01 (P=7.210(-8), OR=4.71).
  
  
  CONCLUSIONS
  Our data suggest that the variation in class II HLA alleles and haplotypes could be a genetic factor involved in the susceptibility of APS syndrome.
authors.affiliation: Immunology Department, Habib Bourguiba Hospital, University of Sfax, Tunisia. hajerfourati@yahoo.fr
authors.forename: H
authors.initials: H
authors.lastname: Fourati
authors.forename: N
authors.initials: N
authors.lastname: Mahfoudh
authors.forename: O
authors.initials: O
authors.lastname: Abida
authors.forename: A
authors.initials: A
authors.lastname: Kammoun
authors.forename: F
authors.initials: F
authors.lastname: Mnif
authors.forename: S
authors.initials: S
authors.lastname: Haddouk
authors.forename: M
authors.initials: M
authors.lastname: Ben Ayed
authors.forename: Y
authors.initials: Y
authors.lastname: Ben Hadj Hmida
authors.forename: N
authors.initials: N
authors.lastname: Rekik
authors.forename: M
authors.initials: M
authors.lastname: Abid
authors.forename: H
authors.initials: H
authors.lastname: Makni
authors.forename: H
authors.initials: H
authors.lastname: Masmoudi
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006683:HLA-DQ Antigens / Q000235:genetics* / Q000276:immunology; D059866:HLA-DQ beta-Chains; D006684:HLA-DR Antigens / Q000235:genetics* / Q000276:immunology; D059811:HLA-DRB1 Chains; D006239:Haplotypes; D006801:Humans; D008297:Male; D008875:Middle Aged; D016884:Polyendocrinopathies, Autoimmune / Q000235:genetics* / Q000276:immunology; D011110:Polymorphism, Genetic; D014416:Tunisia / Q000453:epidemiology; D055815:Young Adult
chemical_list: D006683:HLA-DQ Antigens; D059866:HLA-DQ beta-Chains; C069051:HLA-DQB1 antigen; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains