title: Single administration effects of ethanol on the distribution of white blood cells in rats.
abstract: Acute single administration effects of ethanol on the distribution of total white blood cells (WBCs), neutrophil, basophil, eosinophil, monocyte and lymphocytes were studied in rats. Acute single administration effects of ethanol on the number of red blood cells (RBCs), hemoglobin concentration and hematocrit were also examined. Male 8-week-old Sprague Dawley rats were randomly divided into the ethanol-administered (ETA) group and the control (CON) group. Two parts of an experiment, 1) 1st experiment : (ethanol dose : 1.0 g/kg body weight), and 2) 2nd experiment : (ethanol dose : 2.0 g/kg body weight) were carried out in rats. The rats were starved to 19:00, and deprived of diet for 12 hr and water for 1 hr before the single administration of ethanol. 1.0 or 2.0 g/kg body weight of ethanol (in 20% (w/w) ethanol) was orally administered to ETA group rats via a stainless stomach tube. In the CON group rats, 0.9% NaCl solution was orally given with the solution volume being equal, in the same manner. Single administration of 1.0 or 2.0 g/kg body weight of ethanol did not change the number of RBCs, hemoglobin concentration and hematocrit. Single administration of 1.0 g/kg body weight of ethanol did not also change the number of WBCs. However, administration of 2.0 g/kg body weight of ethanol increased significantly the number of neutrophil, basophil, monocyte and total WBCs without changing the number of eosinophil and lymphocytes. These results suggest that single administration of 2.0 g/kg body weight of ethanol to rats increased markedly the number of the natural immunity cells without changing the number of acquired cells.
authors.affiliation: School of Medicine, University of Yamanashi, 1110 Shimokatou, Chuo, Yamanashi 409-3898, Japan.
authors.forename: Yu
authors.initials: Y
authors.lastname: Kawashima
authors.forename: Yui
authors.initials: Y
authors.lastname: Someya
authors.forename: Ken
authors.initials: K
authors.lastname: Shirato
authors.forename: Shogo
authors.initials: S
authors.lastname: Sato
authors.forename: Hisashi
authors.initials: H
authors.lastname: Ideno
authors.forename: Kyoko
authors.initials: K
authors.lastname: Kobayashi
authors.forename: Kaoru
authors.initials: K
authors.lastname: Tachiyashiki
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Imaizumi
mesh_terms: D000818:Animals; D001772:Blood Cell Count; D002492:Central Nervous System Depressants / Q000633:toxicity*; D004912:Erythrocytes / Q000187:drug effects; D000431:Ethanol / Q000633:toxicity*; D006400:Hematocrit; D006403:Hematologic Tests; D006454:Hemoglobins / Q000187:drug effects; D007962:Leukocytes / Q000187:drug effects* / Q000473:pathology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D023382:Toxicity Tests, Acute
chemical_list: D002492:Central Nervous System Depressants; D006454:Hemoglobins; D000431:Ethanol