title: Prophylactic effect of a therapeutic vaccine against TB based on fragments of Mycobacterium tuberculosis.
abstract: The prophylactic capacity of the RUTI® vaccine, based on fragmented cells of Mycobacterium tuberculosis, has been evaluated in respect to aerosol challenge with virulent bacilli. Subcutaneous vaccination significantly reduced viable bacterial counts in both lungs and spleens of C57Bl mice, when challenged 4 weeks after vaccination. RUTI® protected the spleen less than BCG. Following a 9 month vaccination-challenge interval, protection was observed for the lungs, but not for the spleen. Survival of infected guinea pigs was prolonged by vaccination given 5 weeks before challenge. Inoculations of RUTI® shortly after infection significantly reduced the viable bacterial counts in the lungs, when compared with infected control mice. Thus, vaccination by RUTI® has potential for both the prophylaxis and immunotherapy of tuberculosis.
authors.affiliation: Unitat de Tuberculosi Experimental, Fundació Institut per a la Investigació en Ciències de la Salut Germans Trias i Pujol, Universitat Autònoma de Barcelona, Crta Badalona, Catalonia, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Vilaplana
authors.forename: Olga
authors.initials: O
authors.lastname: Gil
authors.forename: Neus
authors.initials: N
authors.lastname: Cáceres
authors.forename: Sergio
authors.initials: S
authors.lastname: Pinto
authors.forename: Jorge
authors.initials: J
authors.lastname: Díaz
authors.forename: Pere-Joan
authors.initials: PJ
authors.lastname: Cardona
mesh_terms: D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology*; D005260:Female; D006168:Guinea Pigs; D051379:Mice; D009169:Mycobacterium tuberculosis / Q000166:cytology* / Q000276:immunology*; D013997:Time Factors; D014376:Tuberculosis / Q000517:prevention & control*; D014611:Vaccination / Q000379:methods*
chemical_list: D001428:Bacterial Vaccines