title: Differentially expressed micoRNAs in human oocytes.
abstract: OBJECTIVE
  To identify differentially expressed microRNAs (miRNAs) and expression patterns of specific miRNAs during meiosis in human oocytes.
  
  
  METHODS
  To identify differentially expressed miRNAs, GV oocytes and MII oocytes matured at conventional FSH levels (5.5 ng/ml) were analyzed by miRNA microarray. Real-time RT-PCR was used to confirm the changed miRNAs. To validate the dynamic changes of miRNAs from GV to MII stages, oocytes were divided into four groups (#1-4), corresponding to GV oocytes, MI oocytes, MII oocytes matured in conventional FSH level and MII oocytes matured in high FSH level (2,000 ng/ml) respectively.
  
  
  RESULTS
  Compared with GV oocytes, MII oocytes exhibited up-regulation of 4 miRNAs (hsa-miR-193a-5p, hsa-miR-297, hsa-miR-625 and hsa-miR-602), and down-regulation of 11 miRNAs (hsa-miR-888*, hsa-miR-212, hsa-miR-662, hsa-miR-299-5p, hsa-miR-339-5p, hsa-miR-20a, hsa-miR-486-5p, hsa-miR-141*, hsa-miR-768-5p, hsa-miR-376a and hsa-miR-15a). RT-PCR analysis of hsa-miR-15a and hsa-miR-20a expression revealed concordant dynamic changes in oocytes from group 1 to group 4.
  
  
  CONCLUSIONS
  Specific miRNAs in human oocytes had dynamic changes during meiosis. High-concentration FSH in IVM medium led to reverse effect on the expression of hsa-miR-15a and hsa-miR-20a.
authors.affiliation: Reproductive Medical Center, the First Affiliated Hospital of Sun Yat-sen University, No 58, Zhongshan Rd 2, Guangzhou, China.
authors.forename: Yan-Wen
authors.initials: YW
authors.lastname: Xu
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.forename: Chen-Hui
authors.initials: CH
authors.lastname: Ding
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.forename: Fang
authors.initials: F
authors.lastname: Gu
authors.forename: Canquan
authors.initials: C
authors.lastname: Zhou
mesh_terms: D005260:Female; D005640:Follicle Stimulating Hormone / Q000008:administration & dosage; D020869:Gene Expression Profiling; D006801:Humans; D059471:In Vitro Oocyte Maturation Techniques / Q000379:methods; D008540:Meiosis / Q000235:genetics; D008677:Metaphase / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics*; D046228:Microarray Analysis; D009865:Oocytes / Q000254:growth & development* / Q000378:metabolism*
chemical_list: C505361:MIRN15 microRNA, human; C534451:MIRN20a microRNA, human; C559285:MIRN602 microRNA, human; D035683:MicroRNAs; D005640:Follicle Stimulating Hormone