title: Adipose tissue-derived stem cells embedded with eNOS restore cardiac function in acute myocardial infarction model.
abstract: This study assessed the potential therapeutic efficacy of endothelial NO syntheses (eNOS)-expressing adipose tissue-derived stem cells (ADSCs) on infarcted hearts. We isolated CD29+, CD44+, CD45- cells from adipose tissue. Multipotent property of ADSCs was characterized by induction to differentiate into myogenic, neurogenic, and endothelic lineages. We hypothesized that combination of eNOS over-expression and transplantation of ADSCs could restore NO bioavailability and improve cardiac function in infarcted hearts. Here with several lines of experimental evidences, we demonstrated that ADSCs with eNOS overexpression induced eNOS expression in host endothelial cells and vascular smooth muscle cells, both in vitro and in vivo. This effect was possibly mediated by calcium signal. Transplantation of ADSCs with eNOS embedded showed great therapeutic efficacy in reduction of infarcted size, compared with normal ADSC. Results of this study suggest that ADSCs could be an attractive vehicle for the exogenous eNOS expression into heart after infarction, which is beneficial to restoration of cardiac function. Paracrine effect by mobilizing the host endothelial cells and smooth muscle cells may be the mechanism underlying the therapeutic effect.
authors.affiliation: Department of Cardiology, the Ninth Hospital Affiliated to Medical College, Shanghai Jiaotong University, ShangHai, China.
authors.forename: Chun-zhi
authors.initials: CZ
authors.lastname: Shi
authors.forename: Xiao-ping
authors.initials: XP
authors.lastname: Zhang
authors.forename: Zhong-wei
authors.initials: ZW
authors.lastname: Lv
authors.forename: Hui-li
authors.initials: HL
authors.lastname: Zhang
authors.forename: Jian-zhong
authors.initials: JZ
authors.lastname: Xu
authors.forename: Zhao-fang
authors.initials: ZF
authors.lastname: Yin
authors.forename: Yu-qin
authors.initials: YQ
authors.lastname: Yan
authors.forename: Chang-qian
authors.initials: CQ
authors.lastname: Wang
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002478:Cells, Cultured; D004195:Disease Models, Animal; D009203:Myocardial Infarction / Q000601:surgery*; D052250:Nitric Oxide Synthase Type III; D051381:Rats; D033581:Stem Cell Transplantation; D013234:Stem Cells; D016610:Tissue Embedding
chemical_list: D052250:Nitric Oxide Synthase Type III