title: Identifying vulnerable pathways in Mycobacterium tuberculosis by using a knockdown approach.
abstract: We constructed recombinant strains of Mycobacterium tuberculosis in which expression of specific genes was downregulated to identify vulnerable drug targets. Growth phenotypes in macrophages and culture were used to rank targets: the dprE1, clpP1, and fadD32 operons were the best targets and glnA1, glnE, pknL, regX3, and senX3 were poor targets.
authors.affiliation: Queen Mary University of London, Barts and the London, London E1 2AT, United Kingdom.
authors.forename: Paul
authors.initials: P
authors.lastname: Carroll
authors.forename: Marie-Claire
authors.initials: MC
authors.lastname: Faray-Kele
authors.forename: Tanya
authors.initials: T
authors.lastname: Parish
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D002478:Cells, Cultured; D055808:Drug Discovery; D015964:Gene Expression Regulation, Bacterial; D055785:Gene Knockdown Techniques; D006801:Humans; D008264:Macrophages / Q000382:microbiology; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D011401:Promoter Regions, Genetic
chemical_list: D001426:Bacterial Proteins