title: Serotype and genotype distributions of pneumococcal carriage isolates recovered from Brazilian children attending day-care centres.
abstract: Pneumococcal nasopharyngeal carriage isolates recovered from Brazilian children attending day-care centres in 2005 were assessed for serotype, genotype and penicillin susceptibility phenotype. As 124 of the 253 isolates (49 %) were characterized previously with respect to serotype and penicillin susceptibility, the primary objectives were to examine clonal associations and penicillin susceptibility within major serotypes and to assess the suitability of conventional multiplex PCR for deducing carriage serotypes within this population. Using a combination of PCR-based serotyping and the Quellung reaction, serotypes were identified for 81 % (205/253) of the isolates, with serogroups or types 14, 6, 23F, 19F and 18 being predominant. Included within the 205 isolates successfully serotyped by PCR were 28 isolates that had become non-viable. Forty-eight isolates were non-typable using both the PCR method and the Quellung reaction. Penicillin non-susceptibility was observed within 16 of the 18 multilocus sequence types detected. Thus, this study provides further evidence from a diverse collection of pneumococcal clones that PCR-based serotype deduction is useful for providing supportive evidence for pneumococcal conjugate vaccine implementation.
authors.affiliation: Institute of Tropical Pathology and Public Health, Federal University of Goiás, Goiânia, Goiás State, Brazil.|Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA.
authors.forename: Fabiana C
authors.initials: FC
authors.lastname: Pimenta
authors.affiliation: Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA.
authors.forename: Maria da Gloria S
authors.initials: MDGS
authors.lastname: Carvalho
authors.affiliation: Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA.
authors.forename: Robert E
authors.initials: RE
authors.lastname: Gertz
authors.affiliation: Institute of Tropical Pathology and Public Health, Federal University of Goiás, Goiânia, Goiás State, Brazil.
authors.forename: Cristyane G B
authors.initials: CGB
authors.lastname: Bastos-Rocha
authors.affiliation: Institute of Tropical Pathology and Public Health, Federal University of Goiás, Goiânia, Goiás State, Brazil.
authors.forename: Luciana S C
authors.initials: LSC
authors.lastname: Oliveira
authors.affiliation: Institute of Tropical Pathology and Public Health, Federal University of Goiás, Goiânia, Goiás State, Brazil.
authors.forename: Laurine
authors.initials: L
authors.lastname: Lacerda Pigosso
authors.affiliation: Institute of Tropical Pathology and Public Health, Federal University of Goiás, Goiânia, Goiás State, Brazil.
authors.forename: Juliane A
authors.initials: JA
authors.lastname: Lima
authors.affiliation: Secretary of Health, Municipality of Goiânia, Goiás State, Brazil.
authors.forename: Caritas
authors.initials: C
authors.lastname: Marquez Franco
authors.affiliation: Institute of Tropical Pathology and Public Health, Federal University of Goiás, Goiânia, Goiás State, Brazil.
authors.forename: Ana Lucia
authors.initials: AL
authors.lastname: Andrade
authors.affiliation: Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA.
authors.forename: Bernard W
authors.initials: BW
authors.lastname: Beall
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001938:Brazil / Q000453:epidemiology; D002353:Carrier State / Q000453:epidemiology* / Q000382:microbiology*; D002656:Child Day Care Centers; D002675:Child, Preschool; D005838:Genotype; D006801:Humans; D007223:Infant; D008826:Microbial Sensitivity Tests; D009305:Nasopharynx / Q000382:microbiology; D010406:Penicillins / Q000494:pharmacology; D011008:Pneumococcal Infections / Q000453:epidemiology* / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods; D012703:Serotyping; D013296:Streptococcus pneumoniae / Q000145:classification* / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D000900:Anti-Bacterial Agents; D010406:Penicillins