title: [Change and significance of Th17 cells and its secretive IL-17 in patients with autoimmune hemolytic anemia].
abstract: OBJECTIVE
  To observe the percentage of Th17 cells in the peripheral blood and levels of plasma IL-17 of patients with autoimmune hemolytic anemia (AIHA)before treatment and after glucocorticoid treatment and to explore their clinical significance.
  
  
  METHODS
  Flow cytometric assay were used to detect the rate of Th17 cells in AIHA patients. The level of plasma IL-17 in AIHA patients were measured by ELISA.
  
  
  RESULTS
  The rate of Th17 cells and the level of plasma IL-17 were significantly increased before treatment as compared with that in normal controls [(2.78 ± 0.59)%, (126.4 ± 11.6)ng/L vs (0.59 ± 0.15)%, (52.3 ± 4.8) ng/L](P < 0.01). After treatment, The percentage of Th17 cells (1.05±0.28)% was significantly decreased (P < 0.01). The same tendency was also found in the levels of plasma IL-17(78.5 ± 6.4) ng/L(P < 0.01).
  
  
  CONCLUSIONS
  The rate of Th17 cells and the lever of plasma IL-17 significantly elevate in AIHA patients. Glucocorticoid might play a role in AIHA treatment by down-regulating Th17 cells number and concentrations of IL-17.
authors.affiliation: Department of Hematology, Yijishan Hospital, Wannan Medical College, Wuhu 241001, China.
authors.forename: He-sheng
authors.initials: HS
authors.lastname: He
authors.forename: Yan-hui
authors.initials: YH
authors.lastname: Xie
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000744:Anemia, Hemolytic, Autoimmune / Q000378:metabolism*; D018929:Cell Culture Techniques; D005260:Female; D005938:Glucocorticoids / Q000494:pharmacology*; D006801:Humans; D020381:Interleukin-17 / Q000097:blood / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D012189:Retrospective Studies; D058504:Th17 Cells / Q000378:metabolism*; D055815:Young Adult
chemical_list: D005938:Glucocorticoids; D020381:Interleukin-17