title: HIVToolbox, an integrated web application for investigating HIV.
abstract: Many bioinformatic databases and applications focus on a limited domain of knowledge federating links to information in other databases. This segregated data structure likely limits our ability to investigate and understand complex biological systems. To facilitate research, therefore, we have built HIVToolbox, which integrates much of the knowledge about HIV proteins and allows virologists and structural biologists to access sequence, structure, and functional relationships in an intuitive web application. HIV-1 integrase protein was used as a case study to show the utility of this application. We show how data integration facilitates identification of new questions and hypotheses much more rapid and convenient than current approaches using isolated repositories. Several new hypotheses for integrase were created as an example, and we experimentally confirmed a predicted CK2 phosphorylation site. Weblink: [http://hivtoolbox.bio-toolkit.com].
authors.affiliation: School of Life Sciences, University of Nevada Las Vegas, Las Vegas, Nevada, United States of America.
authors.forename: David
authors.initials: D
authors.lastname: Sargeant
authors.forename: Sandeep
authors.initials: S
authors.lastname: Deverasetty
authors.forename: Yang
authors.initials: Y
authors.lastname: Luo
authors.forename: Angel
authors.initials: A
authors.lastname: Villahoz Baleta
authors.forename: Stephanie
authors.initials: S
authors.lastname: Zobrist
authors.forename: Viraj
authors.initials: V
authors.lastname: Rathnayake
authors.forename: Jacqueline C
authors.initials: JC
authors.lastname: Russo
authors.forename: Jay
authors.initials: J
authors.lastname: Vyas
authors.forename: Mark A
authors.initials: MA
authors.lastname: Muesing
authors.forename: Martin R
authors.initials: MR
authors.lastname: Schiller
mesh_terms: D001665:Binding Sites; D047390:Casein Kinase II / Q000378:metabolism; D019295:Computational Biology / Q000379:methods*; D030562:Databases, Protein; D019427:HIV Integrase / Q000737:chemistry / Q000378:metabolism; D015497:HIV-1 / Q000201:enzymology / Q000502:physiology; D020407:Internet; D008958:Models, Molecular; D010766:Phosphorylation; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D018511:Systems Integration; D014584:User-Computer Interface
chemical_list: D047390:Casein Kinase II; D019427:HIV Integrase