title: Pseudomonas baetica sp. nov., a fish pathogen isolated from wedge sole, Dicologlossa cuneata (Moreau).
abstract: Five Gram-negative bacterial isolates, recovered from an outbreak that occurred in March 2006 in Huelva, Spain, affecting adult diseased cultured wedge sole [Dicologlossa cuneata (Moreau)], were characterized phenotypically and genotypically in order to clarify their taxonomic position. On the basis of 16S rRNA gene sequence analysis, the isolates were included in the genus Pseudomonas, within the Pseudomonas fluorescens-related species group, their closest relatives being the Pseudomonas jessenii and Pseudomonas koreensis subgroups. The highest sequence similarities were recorded with the type strains of Pseudomonas reinekei, P. moorei, P. umsongensis, P. jessenii and P. mohnii (99.4-99.3 % similarity). Sequence analysis of the housekeeping genes gyrB and rpoD clearly differentiated the isolates from currently described Pseudomonas species, the highest sequence similarities recorded to type strains being below 95 % for both genes. Phylogenetic analysis using concatenated sequences of the three genes showed Pseudomonas moraviensis DSM 16007T and P. koreensis DSM 16610T as the closest reference strains. DNA-DNA hybridization assays with related strains confirmed that these isolates belong to a novel species of the genus Pseudomonas, for which the name Pseudomonas baetica sp. nov. is proposed. The type strain is strain a390T (=CECT 7720T=LMG 25716T). The novel species could be easily distinguished from phylogenetically related species by several phenotypic characteristics, including gelatin hydrolysis, acid production from glucose and growth at 6 % NaCl. Virulence assays revealed that the novel species is pathogenic for wedge sole.
authors.affiliation: IFAPA Centro Agua del Pino, Junta de Andalucia, Carretera El Portil-El Rompido s/n, 21450 Cartaya, Huelva, Spain.
authors.forename: Jose R
authors.initials: JR
authors.lastname: López
authors.affiliation: Departamento de Microbiología y Parasitología, Facultad de Biología e Instituto de Acuicultura, Universidad de Santiago, 15782 Santiago de Compostela, Spain.
authors.forename: Ana L
authors.initials: AL
authors.lastname: Diéguez
authors.affiliation: Departamento de Microbiología y Parasitología, Facultad de Biología e Instituto de Acuicultura, Universidad de Santiago, 15782 Santiago de Compostela, Spain.
authors.forename: Alejandra
authors.initials: A
authors.lastname: Doce
authors.affiliation: IFAPA Centro Agua del Pino, Junta de Andalucia, Carretera El Portil-El Rompido s/n, 21450 Cartaya, Huelva, Spain.
authors.forename: Elena
authors.initials: E
authors.lastname: De la Roca
authors.affiliation: Departamento de Genética, Facultad de Ciencias, Universidad de Granada, 18071 Granada, Spain.
authors.forename: Roberto
authors.initials: R
authors.lastname: De la Herran
authors.affiliation: IFAPA Centro Agua del Pino, Junta de Andalucia, Carretera El Portil-El Rompido s/n, 21450 Cartaya, Huelva, Spain.
authors.forename: Jose I
authors.initials: JI
authors.lastname: Navas
authors.affiliation: Departamento de Microbiología y Parasitología, Facultad de Biología e Instituto de Acuicultura, Universidad de Santiago, 15782 Santiago de Compostela, Spain.
authors.forename: Alicia E
authors.initials: AE
authors.lastname: Toranzo
authors.affiliation: Departamento de Microbiología y Parasitología, Facultad de Biología e Instituto de Acuicultura, Universidad de Santiago, 15782 Santiago de Compostela, Spain.
authors.forename: Jesus L
authors.initials: JL
authors.lastname: Romalde
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005393:Fish Diseases / Q000382:microbiology; D005412:Flatfishes / Q000382:microbiology*; D005798:Genes, Bacterial; D008099:Liver / Q000382:microbiology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D011549:Pseudomonas / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013030:Spain
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S