title: Comparative proteomic analysis of Fasciola hepatica juveniles and Schistosoma bovis schistosomula.
abstract: Protein interactions between host and parasites can influence the infection success and severity. The aim of this investigation was to identify the proteins from two trematodes potentially localized at the host-parasite interface. We performed the proteomic profiles from in vivo obtained immature lung stage Schistosoma bovis schistosomula and in vitro excysted juveniles from Fasciola hepatica, parasites of ruminants and man usually giving rise to chronic infections. Proteomes from those parasites were obtained after digestion with trypsin and the peptides generated were identified by mass spectrometry, both before and after parasites' treatment with 70% methanol. The comparison of the two proteome sets from each parasite and between them, the analysis of their relative abundance and of their potential exposure to the host from living parasites, together with the specific immunolocalization of two of the identified molecules, show that this approach could assist in the identification of parasite exposed proteins and in the definition of molecules common for the two parasites with potential interaction with the host. Further characterization of these molecules could guide to define new common anti-parasitic targets and potential vaccine candidates.
authors.affiliation: Parasitology Laboratory, IRNASA (CSIC), Cordel de Merinas, 40-52, 37008 Salamanca, Spain.
authors.forename: E
authors.initials: E
authors.lastname: De la Torre Escudero
authors.forename: R
authors.initials: R
authors.lastname: Manzano-Román
authors.forename: L
authors.initials: L
authors.lastname: Valero
authors.forename: A
authors.initials: A
authors.lastname: Oleaga
authors.forename: R
authors.initials: R
authors.lastname: Pérez-Sánchez
authors.forename: A
authors.initials: A
authors.lastname: Hernández-González
authors.forename: M
authors.initials: M
authors.lastname: Siles-Lucas
mesh_terms: D000818:Animals; D005210:Fasciola hepatica / Q000737:chemistry*; D015801:Helminth Proteins / Q000032:analysis*; D006790:Host-Parasite Interactions; D013058:Mass Spectrometry; D020543:Proteome / Q000032:analysis*; D012547:Schistosoma / Q000737:chemistry
chemical_list: D015801:Helminth Proteins; D020543:Proteome