title: Starch with high amylose content and low in vitro digestibility increases intestinal nutrient flow and microbial fermentation and selectively promotes bifidobacteria in pigs.
abstract: Diets containing different starch types can affect enzymatic digestion of starch and thereby starch availability for microbial fermentation in the gut. However, the role of starch chemistry in nutrient digestion and flow and microbial profile has been poorly explained. Eight ileal-cannulated pigs (29.4 ± 0.9 kg body weight) were fed 4 diets containing 70% purified starch (amylose content, <5, 20, 28, and 63%; reflected by in vitro maximal digestion rate; 1.06, 0.73, 0.38, and 0.22%/min, respectively) in a replicated 4 × 4 Latin square. Ileal and fecal starch output, postileal crude protein yield, fecal total SCFA and total butyrate content, and gene copies of Bifidobacterium spp. in feces were higher (P < 0.05) when pigs consumed the slowly digestible starch diet than the remaining 3 starch diets. The in vitro starch digestion rate had a negative, nonlinear relationship with ileal starch flow (R(2) = 0.98; P < 0.001). Ileal starch flow was positively related to Bifidobacterium spp. (R(2) = 0.27; P < 0.01), Lactobacillus group (R(2) = 0.22; P < 0.01), and total butyrate content (R(2) = 0.46; P < 0.01) but was not related to Enterobacteriaceae (R(2) < 0.00; P = 0.92). In conclusion, starch with high amylose content and low in vitro digestibility increased postileal nutrient flow and microbial fermentation and selectively promoted Bifidobacterium spp. in the distal gut.
authors.affiliation: University of Alberta, Edmonton, Alberta T6G 2P5, Canada.
authors.forename: Prajwal R
authors.initials: PR
authors.lastname: Regmi
authors.forename: Barbara U
authors.initials: BU
authors.lastname: Metzler-Zebeli
authors.forename: Michael G
authors.initials: MG
authors.lastname: Gänzle
authors.forename: Theo A T G
authors.initials: TA
authors.lastname: van Kempen
authors.forename: Ruurd T
authors.initials: RT
authors.lastname: Zijlstra
mesh_terms: D000688:Amylose / Q000008:administration & dosage / Q000032:analysis; D000821:Animal Feed / Q000032:analysis; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D001644:Bifidobacterium / Q000254:growth & development / Q000378:metabolism*; D002087:Butyrates / Q000378:metabolism; D004063:Digestion / Q000187:drug effects; D005285:Fermentation / Q000187:drug effects; D007082:Ileum / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology; D007408:Intestinal Absorption / Q000187:drug effects; D054892:Metagenome / Q000187:drug effects; D013213:Starch / Q000008:administration & dosage / Q000737:chemistry* / Q000493:pharmacokinetics*; D034421:Sus scrofa
chemical_list: D002087:Butyrates; D013213:Starch; D000688:Amylose