title: Multiplex single-nucleotide polymorphism typing of the human Y chromosome using TaqMan probes.
abstract: BACKGROUND
  The analysis of human Y-chromosome variation in the context of population genetics and forensics requires the genotyping of dozens to hundreds of selected single-nucleotide polymorphisms (SNPs). In the present study, we developed a 121-plex (121 SNPs in a single array) TaqMan array capable of distinguishing most haplogroups and subhaplogroups on the Y-chromosome human phylogeny in Europe.
  
  
  RESULTS
  We present data from 264 samples from several European areas and ethnic groups. The array developed in this study shows >99% accuracy of assignation to the Y human phylogeny (with an average call rate of genotypes >96%).
  
  
  CONCLUSIONS
  We have created and evaluated a robust and accurate Y-chromosome multiplex which minimises the possible errors due to mixup when typing the same sample in several independent reactions.
authors.affiliation: Institut de Biologia Evolutiva (CSIC-UPF), Departament de Ciències Experimentals i de la Salut, Universitat Pompeu Fabra, Doctor Aiguader 88, E-08003 Barcelona, Spain. david.comas@upf.edu.
authors.forename: Begoña
authors.initials: B
authors.lastname: Martínez-Cruz
authors.forename: Janet
authors.initials: J
authors.lastname: Ziegle
authors.forename: Paula
authors.initials: P
authors.lastname: Sanz
authors.forename: Graciela
authors.initials: G
authors.lastname: Sotelo
authors.forename: Roger
authors.initials: R
authors.lastname: Anglada
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Plaza
authors.forename: David
authors.initials: D
authors.lastname: Comas