title: Functional analysis of the HS185 regulatory element in the rice HSP70 promoter.
abstract: A series of HSP70 promoter deletion constructs was established. Analysis of beta-glucuronidase activities from the promoter deletion constructs in transient expression assays identified a cis-element, located from -493 to -308 bp upstream of the ATG start site. This element was designated as HS185 and has a crucial role in HSP70 promoter activity. HS185 has some characteristics of a miniature inverted-repeat transposable element (MITE), such as terminal inverted repeats (TIRs) (GGTCCCACA) and a putative target site duplication. There are 362 copies of homologous sequences of HS185 in the rice genome, which are preferentially distributed to non-coding regions. Based on these sequence features, we propose that HS185 is an uncharacterized rice MITE, possibly derived from the rice transposon Mutator-like element VIII family. Further transient expression assays showed that HS185 inhibited the enhancer activity of the cauliflower mosaic virus 35S promoter. These results demonstrate that not only is HS185 necessary for HSP70 promoter activity, but it also has a functional role as an insulator. This study explored new regulatory functions of non-coding repeat sequences in rice.
authors.affiliation: State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Yu-Man
authors.initials: YM
authors.lastname: Zhang
authors.forename: Yu-Mei
authors.initials: YM
authors.lastname: Zheng
authors.forename: Na
authors.initials: N
authors.lastname: Xiao
authors.forename: Li-Na
authors.initials: LN
authors.lastname: Wang
authors.forename: Yao
authors.initials: Y
authors.lastname: Zhang
authors.forename: Rong-Xiang
authors.initials: RX
authors.lastname: Fang
authors.forename: Xiao-Ying
authors.initials: XY
authors.lastname: Chen
mesh_terms: D020029:Base Pairing; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D017796:Caulimovirus / Q000235:genetics; D003001:Cloning, Molecular; D019295:Computational Biology; D004251:DNA Transposable Elements / Q000235:genetics*; D015967:Gene Expression Regulation, Viral / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D055029:Inverted Repeat Sequences / Q000235:genetics; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics*; D018515:Plant Leaves / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D050436:Regulatory Elements, Transcriptional / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014026:Nicotiana / Q000378:metabolism
chemical_list: D004251:DNA Transposable Elements; D018840:HSP70 Heat-Shock Proteins