title: Automatic cytometric device using multiple wavelength excitations.
abstract: Precise identification of eosinophils, basophils, and specific subpopulations of blood cells (B lymphocytes) in an unconventional automatic hematology analyzer is demonstrated. Our specific apparatus mixes two excitation radiations by means of an acousto-optics tunable filter to properly control fluorescence emission of phycoerythrin cyanin 5 (PC5) conjugated to antibodies (anti-CD20 or anti-CRTH2) and Thiazole Orange. This way our analyzer combining techniques of hematology analysis and flow cytometry based on multiple fluorescence detection, drastically improves the signal to noise ratio and decreases the spectral overlaps impact coming from multiple fluorescence emissions.
authors.affiliation: HORIBA Medical, Parc Euromédecine, Rue du Caducée, 34000 Montpellier Cedex 4, France. nelly.rongeat@horiba.com
authors.forename: Nelly
authors.initials: N
authors.lastname: Rongeat
authors.forename: Sylvain
authors.initials: S
authors.lastname: Ledroit
authors.forename: Laurence
authors.initials: L
authors.lastname: Chauvet
authors.forename: Didier
authors.initials: D
authors.lastname: Cremien
authors.forename: Alexandra
authors.initials: A
authors.lastname: Urankar
authors.forename: Vincent
authors.initials: V
authors.lastname: Couderc
authors.forename: Philippe
authors.initials: P
authors.lastname: Nérin
mesh_terms: D000465:Algorithms; D001772:Blood Cell Count / Q000295:instrumentation*; D002478:Cells, Cultured; D005434:Flow Cytometry / Q000295:instrumentation*; D006801:Humans; D007089:Image Enhancement / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D010363:Pattern Recognition, Automated / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity