title: Development and in vitro assay of oxidative stress modifying formulations for wound healing promotion.
abstract: Often presented as metabolism byproducts, reactive oxygen species are linked to detrimental effects such as chronic wound, mutagenesis, cancer and skin ageing. However, recent in vitro and in vivo observations suggest that ROS, and mainly hydrogen peroxide, interfere with cell signaling acting like second messenger and inducing adaptive responses. This is particularly observed in skin wound healing where cells are exposed to H₂O₂ following injury. In this study, we developed and characterized an innovative formulation producing H₂O₂ at low concentrations, in order to mimic physiological inflammation phase. Then, this pro-oxidative formulation (CAM-GOx) was assayed in vitro on keratinocytes cell culture, compared to the blank formulation (CAM) and the anti-oxidative formulation (CAM-CAT) to assess whether oxidative stress was implied or not in cellular responses.
authors.affiliation: University of Lyon, Physiological and Pathological Functions of the Cutaneous Barrier, Laboratory for R&D in Industrial Pharmaceutical Technologies, Lyon, France. Nicolas.atrux@gmail.com
authors.forename: Nicolas
authors.initials: N
authors.lastname: Atrux-Tallau
authors.forename: Sylvie
authors.initials: S
authors.lastname: Callejon
authors.forename: Camille
authors.initials: C
authors.lastname: Migdal
authors.forename: Karine
authors.initials: K
authors.lastname: Padois
authors.forename: Valérie
authors.initials: V
authors.lastname: Bertholle
authors.forename: Alain
authors.initials: A
authors.lastname: Denis
authors.forename: Marlène
authors.initials: M
authors.lastname: Chavagnac-Bonneville
authors.forename: Marek
authors.initials: M
authors.lastname: Haftek
authors.forename: Françoise
authors.initials: F
authors.lastname: Falson
authors.forename: Fabrice
authors.initials: F
authors.lastname: Pirot
mesh_terms: D000464:Alginates; D054443:Cell Migration Assays; D002478:Cells, Cultured; D048271:Chitosan; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D015603:Keratinocytes / Q000166:cytology; D008863:Microspheres; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D014945:Wound Healing / Q000502:physiology*
chemical_list: D000464:Alginates; D017382:Reactive Oxygen Species; D042461:Vascular Endothelial Growth Factor A; D048271:Chitosan; D006861:Hydrogen Peroxide