title: BAD-LAMP is a novel biomarker of nonactivated human plasmacytoid dendritic cells.
abstract: The brain and dendritic cell (BAD)-associated lysosome-associated membrane protein (LAMP)-like molecule (BAD-LAMP, c20orf103, UNC-46) is a newly identified member of the family of LAMPs. BAD-LAMP expression in the mouse is confined to neurons. We demonstrate here that in humans, BAD-LAMP can specifically be found in the type I IFN-producing plasmacytoid dendritic cells (pDCs). Human BAD-LAMP is localized in the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) of freshly isolated CD123(+) pDCs and is rapidly lost upon activation by unmethylated cytosine-phosphate-guanine (CpG) oligonucleotides. The restricted pattern of BAD-LAMP expression allows for the rapid identification of normal and leukemic human pDCs in tissues and blood.
authors.affiliation: Centre d'Immunologie de Marseille-Luminy, Université de la Méditerranée, Marseille, France.
authors.forename: Axel
authors.initials: A
authors.lastname: Defays
authors.forename: Alexandre
authors.initials: A
authors.lastname: David
authors.forename: Aude
authors.initials: A
authors.lastname: de Gassart
authors.forename: Francesca
authors.initials: F
authors.lastname: De Angelis Rigotti
authors.forename: Till
authors.initials: T
authors.lastname: Wenger
authors.forename: Voahirana
authors.initials: V
authors.lastname: Camossetto
authors.forename: Pierre
authors.initials: P
authors.lastname: Brousset
authors.forename: Tony
authors.initials: T
authors.lastname: Petrella
authors.forename: Marc
authors.initials: M
authors.lastname: Dalod
authors.forename: Evelina
authors.initials: E
authors.lastname: Gatti
authors.forename: Philippe
authors.initials: P
authors.lastname: Pierre
mesh_terms: D015415:Biomarkers / Q000378:metabolism*; D001921:Brain / Q000378:metabolism; D003713:Dendritic Cells / Q000166:cytology / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000378:metabolism; D011992:Endosomes / Q000378:metabolism; D006056:Golgi Apparatus / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D007377:Interleukin-3 / Q000378:metabolism; D053650:Interleukin-3 Receptor alpha Subunit / Q000378:metabolism; D007938:Leukemia / Q000378:metabolism / Q000473:pathology*; D051907:Lysosomal Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D009841:Oligonucleotides / Q000235:genetics / Q000378:metabolism; D014162:Transfection
chemical_list: D015415:Biomarkers; C115022:IL3RA protein, human; D007377:Interleukin-3; D053650:Interleukin-3 Receptor alpha Subunit; C561580:LAMP5 protein, human; D051907:Lysosomal Membrane Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D009841:Oligonucleotides; C454130:UNC93B1 protein, human