title: Identification of new polymorphic microsatellite markers in the NA1 and NA2 lineages of Phytophthora ramorum.
abstract: Phytophthora ramorum is a recently introduced pathogen in Europe and North America consisting of three clonal lineages. Due to the limited intralineage genetic variation, only a few polymorphic markers are available for use in studies involving the epidemiology and evolution of P. ramorum. A total of 159 primer pairs for candidate polymorphic SSR loci were tested with universal labeling. Four polymorphic microsatellite loci were identified within the NA1 lineage and one within the NA2 lineage, demonstrating the power and flexibility of the screening technique. The markers may significantly increase the number of genotypes that can be identified and as such can help better characterize the North American lineages of P. ramorum.
authors.affiliation: Institute for Agricultural and Fisheries Research (ILVO), Plant Sciences Unit, Crop Protection, Burg. Van Gansberghelaan 96 bus 2, 9820 Merelbeke, Belgium.
authors.forename: Annelies
authors.initials: A
authors.lastname: Vercauteren
authors.forename: Meg
authors.initials: M
authors.lastname: Larsen
authors.forename: Erica
authors.initials: E
authors.lastname: Goss
authors.forename: Niklaus J
authors.initials: NJ
authors.lastname: Grünwald
authors.forename: Martine
authors.initials: M
authors.lastname: Maes
authors.forename: Kurt
authors.initials: K
authors.lastname: Heungens
mesh_terms: D001483:Base Sequence; D014644:Genetic Variation; D005838:Genotype; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D010838:Phytophthora / Q000145:classification / Q000235:genetics*; D011110:Polymorphism, Genetic