title: Immunohistochemical determination of ETS-1 oncoprotein expression in urothelial carcinomas of the urinary bladder.
abstract: ETS-1 protooncogene is an important transcription factor that plays a role in the regulation of physiological processes, such as cell proliferation and differentiation. ETS-1 is thought to be related to the growth of carcinoma cells by its regulation of the transcription of matrix metalloproteinases and urokinase-type plasminogen activator. In this study, we aimed to investigate the expression pattern of ETS-1 oncoprotein in urothelial carcinomas of the urinary bladder and determine its relationship with histopathologic parameters, including tumor grade and stage. One hundred six specimens of urothelial carcinoma and a total of 14 normal urothelium were analyzed immunohistochemically with anti-ETS-1 monoclonal antibody. The normal urothelium showed positive ETS-1 immunostaining. ETS-1 expression remained high in low-grade and noninvasive tumors, whereas it frequently decreased in high-grade or invasive carcinomas. Interestingly, ETS-1 was highly expressed in the basal cell layer of the noninvasive urothelial carcinomas. ETS-1 expression showed a strong negative correlation with the tumor grade (P<0.001; r, -0.67) and stage (P<0.001; r, -0.75). The nonmuscle-invasive tumors (pTa+pT1) and noninvasive tumors (pTa) had significantly higher ETS-1 expression than the muscle-invasive tumors (pT2; P<0.001) and invasive tumors (pT1+pT2; P<0.001), respectively. Results of our study show that decreased ETS-1 expression is significantly associated with high grade and advanced stage in urothelial carcinomas of the urinary bladder, and that the downregulation of ETS-1 expression may be a marker of the aggressiveness of such malignancies.
authors.affiliation: Department of Pathology, Izmir Ataturk Research and Training Hospital, Izmir, Turkey. aysegulakder@gmail.com
authors.forename: Aysegul
authors.initials: A
authors.lastname: Sari
authors.forename: Aylin
authors.initials: A
authors.lastname: Calli
authors.forename: Sacit Nuri
authors.initials: SN
authors.lastname: Gorgel
authors.forename: Aysegul Aksoy
authors.initials: AA
authors.lastname: Altinboga
authors.forename: Cengiz
authors.initials: C
authors.lastname: Kara
authors.forename: Cetin
authors.initials: C
authors.lastname: Dincel
authors.forename: Fulya
authors.initials: F
authors.lastname: Cakalagaoglu
mesh_terms: D000328:Adult; D000368:Aged; D002277:Carcinoma / Q000378:metabolism / Q000473:pathology; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D050784:Proto-Oncogene Protein c-ets-1 / Q000096:biosynthesis*; D012189:Retrospective Studies; D001749:Urinary Bladder Neoplasms / Q000378:metabolism / Q000473:pathology; D019459:Urothelium / Q000378:metabolism / Q000473:pathology
chemical_list: C494041:ETS1 protein, human; D050784:Proto-Oncogene Protein c-ets-1