title: Analysis of the cross-reactivity of various 56 kDa recombinant protein antigens with serum samples collected after Orientia tsutsugamushi infection by ELISA.
abstract: Orientia tsutsugamushi, the etiologic agent of scrub typhus, has a highly expressed and immunodominant 56-kD outer membrane protein. This protein is one of the leading candidates for diagnosis and vaccine development for scrub typhus. Previous studies using recombinant 56-kD protein (r56s) derived from Karp strain (Kpr56) in a mouse model have shown good homologous protection but only moderate to poor heterologous protection. We evaluated the cross-reactivity of recombinant 56-kD proteins from Karp, Kato, Gilliam, TA763, and three chimeric 56-kD proteins. Not all r56s are equally reactive with strain-specific serum samples. These data provide a first glance of how reactive these r56s are toward the antiserum of different strains and which r56 exhibits the broadest reactivity. A formulation of this combination has the potential to provide broad protection against the heterologous challenge and to be used in a highly sensitive diagnostic assay.
authors.affiliation: Viral and Rickettsial Diseases Department, Infectious Diseases Directorate, Naval Medical Research Center, 503 Robert Grant Ave., Silver Spring, MD 20910, USA. chien-chung.chao@med.navy.mil
authors.forename: Chien-Chung
authors.initials: CC
authors.lastname: Chao
authors.forename: Erin S
authors.initials: ES
authors.lastname: Huber
authors.forename: Terrisita B
authors.initials: TB
authors.lastname: Porter
authors.forename: Zhiwen
authors.initials: Z
authors.lastname: Zhang
authors.forename: Wei-Mei
authors.initials: WM
authors.lastname: Ching
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000097:blood / Q000276:immunology*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D051379:Mice; D008969:Molecular Sequence Data; D012285:Orientia tsutsugamushi / Q000276:immunology* / Q000472:pathogenicity; D011994:Recombinant Proteins / Q000276:immunology; D012612:Scrub Typhus / Q000276:immunology*; D013045:Species Specificity
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D011994:Recombinant Proteins