title: Renal small cell oncocytoma with pseudorosettes A histomorphologic, immunohistochemical, and molecular genetic study of 10 cases.
abstract: A cohort of a heretofore not described rare subtype of renal oncocytoma, small cell oncocytoma with pseudorosettes is presented. Patients were 6 women and 4 men with ages ranging from 51 to 76 years. The tumors displayed areas composed of small cells ("oncoblasts") featuring scant cytoplasm and small, round monomorphic nuclei. The small cell areas constituted 15% to 60% of the total tumor volume (mean, 28.5%; median, 22.5%). No necrosis or mitotic activity was discerned. All tumors also contained areas composed of characteristic oncocytes comprising 40% to 85% of the total tumor volume. In all cases, a varying number of pseudorosettes were identified. The pseudorosettes were composed of small globules of (periodic acid-Schiff-positive) hyaline basal membrane-like material surrounded by small "oncoblastic" cells. The immunohistochemical profile was variable, including at least focal positivity for AE1-3 (10/10), cytokeratin 7 (7/10), epithelial membrane antigen (10/10), c-kit (6/10), antimitochondrial antigen (MIA;10/10), PAX-2 (9/10), AMACR (racemase;6/10), CD10 (5/10), parvalbumin (8/10), vimentin (6/10), claudin 7 (10/10), and claudin 8 (3/10). No immunoreactivity for carbonic anhydrase 9, HMB-45, S-100A1, and TFE3 was documented. We found no differences in the immunophenotype in the small cell oncocytes/oncoblasts that formed pseudorosettes and those that did not. However, there were differences in the immunohistochemical profile of classic oncocytes and small cell oncocytes/oncoblasts. Using array comparative genomic hybridization, no chromosomal changes were identified in any of the cases examined (n = 3). No numerical changes of chromosomes 7 and 17 were revealed on fluorescence in situ hybridization analysis (n = 3). In conclusion, we herein present the first study on small cell renal oncocytomas with formation of pseudorosettes. This is a rare subtype of oncocytoma, which may, especially on a core biopsy, present differential diagnostic difficulties. The immunohistochemical profile of these tumors is variable and differs in significant respects from that of conventional renal oncocytoma. Awareness of this entity and its immunohistochemical variability should help in distinguishing this rare tumor from malignant tumors with similar (small cell) histomorphologic features. All tumors behaved in a benign fashion during follow-up (mean, 3.1 years; median, 1 year).
authors.affiliation: Department of Pathology, Charles University and University Hospital, 304 60 Plzen, Czech Republic; Department of Pathology, National University Health System, 119074 Singapore.
authors.forename: Fredrik
authors.initials: F
authors.lastname: Petersson
authors.forename: Radek
authors.initials: R
authors.lastname: Síma
authors.forename: Petr
authors.initials: P
authors.lastname: Grossmann
authors.forename: Michal
authors.initials: M
authors.lastname: Michal
authors.forename: Naoto
authors.initials: N
authors.lastname: Kuroda
authors.forename: Milan
authors.initials: M
authors.lastname: Hora
authors.forename: Ximing
authors.initials: X
authors.lastname: Yang
authors.forename: Zdeněk
authors.initials: Z
authors.lastname: Kinkor
authors.forename: Sandra
authors.initials: S
authors.lastname: Trivunic
authors.forename: Radim
authors.initials: R
authors.lastname: Zalud
authors.forename: Maris
authors.initials: M
authors.lastname: Sperga
authors.forename: Zane
authors.initials: Z
authors.lastname: Jaunmuktane
authors.forename: Jindřich
authors.initials: J
authors.lastname: Branžovský
authors.forename: Jiří
authors.initials: J
authors.lastname: Ferda
authors.forename: Ondrej
authors.initials: O
authors.lastname: Hes
mesh_terms: D018249:Adenoma, Oxyphilic / Q000235:genetics / Q000473:pathology*; D000368:Aged; D002292:Carcinoma, Renal Cell / Q000235:genetics / Q000473:pathology*; D055028:Comparative Genomic Hybridization; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007680:Kidney Neoplasms / Q000235:genetics / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D024862:Oxyphil Cells / Q000473:pathology