title: Simultaneous detection and subtyping of H274Y-positive influenza A (H1N1) using pyrosequencing.
abstract: BACKGROUND
  We investigated the frequency of H274Y-positive swine-origin 2009 A (H1N1) influenza virus outbreak in Thailand during May-August 2009.
  
  
  METHODS
  This study sought to find Oseltamivir resistance mutation H274Y by using pyrosequencing.
  
  
  RESULTS
  From 8,710 real-time RT-PCR swine-origin 2009 A(H1N1) influenza virus-positive specimens, 100 randomly selected samples identified one such virus with H274Y mutation  using pyrosequencing.
  
  
  CONCLUSIONS
  The patient probably acquired oseltamivir resistance from natural variation, since he had never received that form of treatment before and recovered from influenza-like symptoms without using anti-influenza drugs.
authors.affiliation: Virology and Molecular Microbiology Unit, Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok. rawct@mahidol.ac.th
authors.forename: Wasun
authors.initials: W
authors.lastname: Chantratita
authors.forename: Chonlaphat
authors.initials: C
authors.lastname: Sukasem
authors.forename: Sayomporn
authors.initials: S
authors.lastname: Sirinavin
authors.forename: Nipaporn
authors.initials: N
authors.lastname: Sankuntaw
authors.forename: Chutatip
authors.initials: C
authors.lastname: Srichantaratsamee
authors.forename: Ekawat
authors.initials: E
authors.lastname: Pasomsub
authors.forename: Kumthorn
authors.initials: K
authors.lastname: Malathum
mesh_terms: D000328:Adult; D019943:Amino Acid Substitution / Q000235:genetics*; D000998:Antiviral Agents / Q000494:pharmacology; D004196:Disease Outbreaks; D024882:Drug Resistance, Viral; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification*; D007251:Influenza, Human / Q000821:virology*; D008297:Male; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D020125:Mutation, Missense; D053139:Oseltamivir / Q000494:pharmacology; D017422:Sequence Analysis, DNA / Q000379:methods; D013785:Thailand; D014773:Virology / Q000379:methods*
chemical_list: D000998:Antiviral Agents; D053139:Oseltamivir