title: [Neuropeptide Y promotes the proliferation and differentiation of 3T3-L1 preadipocytes].
abstract: OBJECTIVE
  To observe the effect of neuropeptide Y on the proliferation and differentiation of 3T3L1 preadipocytes and detect the mechanics.
  
  
  METHODS
  3T3-L1 preadipocytes were induced to differentiation by the cocktail medium containing 3-isobutyl-1-methylxanthine (IBMX), dexamethasone and insulin. After 2 days, the cells were divided into 3 groups: the control group(without inducer), the routine group(insulin used as inducer) and the experiment group(10(-8), 10(-9), 10(-10) mol/L NPY used as inducer). At day 7 and 12, cell differentiation was observed by phase contrast microscope. At day 12, Oil red O staining was performed. Proliferation capacities of 3T3L1 cells were assessed by MTT. Western blot was used to know the protein level of peroxisome proliferator-activated receptor gamma (PPAR-γ) and The CCAAT/enhancer-binding protein-α(C/EBP-α).
  
  
  RESULTS
  NPY 10(-8) mol/L promotes the differentiation of 3T3L1 preadipocytes, 10(-9) mol/L, 10(-8) mol/L NPY increased 3T3-L1 cells proliferation. The protein levels of PPAR-γ and C/EBP-α were up regulated by 10(-8) mol/L NPY.
  
  
  CONCLUSIONS
  Neuropeptide Y promotes the differentiation and Proliferation of 3T3-L1 cells, this effect may be related to up-regulation of PPAR-γ and C/EBP-α.
authors.affiliation: Endocrinology, Third Xiangya Hospital, Central South University, Changsha 410013, China. liumin330@hotmail.com
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.forename: Ping-An
authors.initials: PA
authors.lastname: Hu
authors.forename: Ke-Xiang
authors.initials: KX
authors.lastname: Ding
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000378:metabolism*; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D007328:Insulin / Q000378:metabolism; D051379:Mice; D009478:Neuropeptide Y / Q000378:metabolism*; D047495:PPAR gamma / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D022763:CCAAT-Enhancer-Binding Protein-alpha; D007328:Insulin; D009478:Neuropeptide Y; D047495:PPAR gamma