title: Sharper low-power STED nanoscopy by time gating.
abstract: Applying pulsed excitation together with time-gated detection improves the fluorescence on-off contrast in continuous-wave stimulated emission depletion (CW-STED) microscopy, thus revealing finer details in fixed and living cells using moderate light intensities. This method also enables super-resolution fluorescence correlation spectroscopy with CW-STED beams, as demonstrated by quantifying the dynamics of labeled lipid molecules in the plasma membrane of living cells.
authors.affiliation: Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Göttingen, Germany.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Vicidomini
authors.forename: Gael
authors.initials: G
authors.lastname: Moneron
authors.forename: Kyu Y
authors.initials: KY
authors.lastname: Han
authors.forename: Volker
authors.initials: V
authors.lastname: Westphal
authors.forename: Haisen
authors.initials: H
authors.lastname: Ta
authors.forename: Matthias
authors.initials: M
authors.lastname: Reuss
authors.forename: Johann
authors.initials: J
authors.lastname: Engelhardt
authors.forename: Christian
authors.initials: C
authors.lastname: Eggeling
authors.forename: Stefan W
authors.initials: SW
authors.lastname: Hell
mesh_terms: D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry; D008027:Light; D008055:Lipids / Q000032:analysis / Q000737:chemistry; D007614:Macropodidae; D008856:Microscopy, Fluorescence / Q000379:methods*; D013997:Time Factors
chemical_list: D008055:Lipids