title: Establishment of a primary pleomorphic xanthoastrocytoma cell line:  in vitro responsiveness to some chemotherapeutics.
abstract: BACKGROUND
  Anaplastic pleomorphic xanthoastrocytoma is an aggressively growing, malignant, and eventually fatal tumor of the central nervous system. Testing chemotherapeutic drug sensitivity under in vitro conditions would be a useful strategy to determine sensitive or resistant drugs for fatal brain cancers.
  
  
  OBJECTIVE
  To establish primary cell cultures of excised tumor tissue from pleomorphic xanthoastrocytoma-bearing patients and to test their sensitivity against various anticancer chemotherapy drugs.
  
  
  METHODS
  Prepared suspensions of the excised tumor tissue from a patient who had a recurrent grade 3 pleomorphic xanthoastrocytoma was cultured in culture dishes until cells began to grow. Immunofluorescent and immunohistochemical visualizations were performed using confocal and light microscopy. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay in comparison with ³H-thymidine incorporation assay was used to test cellular toxicity of several anticancer drugs.
  
  
  RESULTS
  We established vigorously growing primary cells of the tumor. Drug sensitivity testing was conducted successfully.
  
  
  CONCLUSIONS
  Primary cell cultures of surgically removed tumor tissues may be useful in studies of cancer biology and chemotherapeutic drug sensitivity for recurrent malignant brain tumors, particularly for anaplastic pleomorphic xanthoastrocytoma.
authors.affiliation: Immunology Research Center, Gazi University, Ankara, Turkey. eubagriacik@yahoo.com
authors.forename: Emin Umit
authors.initials: EU
authors.lastname: Bagriacik
authors.forename: Mustafa Kemali
authors.initials: MK
authors.lastname: Baykaner
authors.forename: Melek
authors.initials: M
authors.lastname: Yaman
authors.forename: Gizem
authors.initials: G
authors.lastname: Sivrikaya
authors.forename: Emre
authors.initials: E
authors.lastname: Durdağ
authors.forename: Hakan
authors.initials: H
authors.lastname: Emmez
authors.forename: Gökçe Öztürk
authors.initials: GÖ
authors.lastname: Fincan
authors.forename: Alp Özgün
authors.initials: AÖ
authors.lastname: Börcek
authors.forename: Ahmet Eren
authors.initials: AE
authors.lastname: Seçen
authors.forename: Sevim
authors.initials: S
authors.lastname: Ercan
mesh_terms: D000293:Adolescent; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001254:Astrocytoma / Q000150:complications / Q000473:pathology*; D001932:Brain Neoplasms / Q000150:complications / Q000473:pathology*; D002452:Cell Count; D049109:Cell Proliferation / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006801:Humans; D008279:Magnetic Resonance Imaging; D008297:Male; D061251:Primary Cell Culture / Q000379:methods*; D013778:Tetrazolium Salts; D013844:Thiazoles; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014407:Tumor Cells, Cultured / Q000187:drug effects* / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D005904:Glial Fibrillary Acidic Protein; D013778:Tetrazolium Salts; D013844:Thiazoles; D014316:Tritium; C022616:thiazolyl blue; D013936:Thymidine