title: A good breath of oxygen for beta-like cells obtained from porcine exocrine pancreatic tissue.
abstract: Ischemia is the most important factor that affects organ survival during harvesting. The two-layer method (TLM) is one of several cold storage solutions that seeks to preserve organs and cells avoiding in vivo and in vitro ischemia. We compared the retrieval of beta-like elements from exocrine pancreatic cells using TLM versus University of Wisconsin (UW) solutions. For this purpose pancreata laparoscopically harvested from 20 female pigs were preserved in UW solution or TLM before digestion. The resulting exocrine cells were divided into 2 groups: the first was cultured in a designed medium to allow differentiation into beta-like cells and the second was cryopreserved before the differentiation process at -196 °C for 8 weeks before culture in the same medium. The results revealed that TLM was better than UW as a preservation solution in terms of beta-cell viability and insulin secretion. We suggest that the use of TLM solution allows one to obtain less damaged cells for research purposes.
authors.affiliation: Dipartimento di Discipline Chirurgiche ed Oncologiche, Università degli Studi di Palermo, A. Mirri, Palermo, Italy.
authors.forename: M C
authors.initials: MC
authors.lastname: Gioviale
authors.forename: G
authors.initials: G
authors.lastname: Damiano
authors.forename: F
authors.initials: F
authors.lastname: Cacciabaudo
authors.forename: V D
authors.initials: VD
authors.lastname: Palumbo
authors.forename: M
authors.initials: M
authors.lastname: Bellavia
authors.forename: G
authors.initials: G
authors.lastname: Cassata
authors.forename: G
authors.initials: G
authors.lastname: Spinelli
authors.forename: G
authors.initials: G
authors.lastname: Buscemi
authors.forename: A I
authors.initials: AI
authors.lastname: Lo Monte
mesh_terms: D000241:Adenosine / Q000494:pharmacology; D000493:Allopurinol / Q000494:pharmacology; D000818:Animals; D002454:Cell Differentiation; D002469:Cell Separation; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D015925:Cryopreservation; D003470:Culture Media / Q000378:metabolism; D005260:Female; D005466:Fluorocarbons / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism; D005978:Glutathione / Q000494:pharmacology; D007328:Insulin / Q000378:metabolism / Q000494:pharmacology; D050417:Insulin-Secreting Cells / Q000187:drug effects* / Q000378:metabolism; D010535:Laparoscopy; D019188:Organ Preservation Solutions / Q000494:pharmacology*; D010100:Oxygen / Q000378:metabolism*; D046790:Pancreas, Exocrine / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010180:Pancreatectomy; D011887:Raffinose / Q000494:pharmacology; D013552:Swine; D013997:Time Factors; D020858:Tissue and Organ Harvesting / Q000379:methods*
chemical_list: D003470:Culture Media; D005466:Fluorocarbons; D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D005978:Glutathione; D005947:Glucose; D000241:Adenosine; D011887:Raffinose; D010100:Oxygen