title: [Effect of UBE2C overexpression on the proliferation of 293T cell line].
abstract: OBJECTIVE
  To establish a 293T cell line with stable expression of UBE2C and to investigate the effect of UBE2C overexpression on the proliferation of 293T cells.
  
  
  METHODS
  Recombinant plasmid pcDNA3.1(-)/UBE2C successfully constructed in previous time was transfected into 293T cells by lipofectamine 2000. After screened with G418 for 4 weeks, Western blot was used to detect the protein level of UBE2C and MTT assay to detect the proliferation of 293T cells.
  
  
  RESULTS
  Compared with pcDNA3.1(-) transfected group, Western blot analysis demonstrated that the level of UBE2C was significantly increased in pcDNA3.1(-)/UBE2C transfected group (P < 0.05). MTT assay showed that 293T cells overexpressing UBE2C proliferated faster than control 293T cells.
  
  
  CONCLUSIONS
  A 293T cell line with UBE2C stable expression is successfully established and UBE2C promoted the proliferation of 293T cells.
authors.affiliation: Department of Lab Medicine, Boai Hospital Affiliated With Southern Medical University, Zhongshan 528403, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Huang
authors.forename: Yu-Lin
authors.initials: YL
authors.lastname: Qiu
authors.forename: Jia-Yu
authors.initials: JY
authors.lastname: Tan
authors.forename: Ya-Feng
authors.initials: YF
authors.lastname: Qiao
authors.forename: Ming
authors.initials: M
authors.lastname: Li
mesh_terms: D002460:Cell Line; D049109:Cell Proliferation; D003001:Cloning, Molecular / Q000379:methods*; D005822:Genetic Vectors; D006801:Humans; D010957:Plasmids; D014162:Transfection; D044763:Ubiquitin-Conjugating Enzymes / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation / Q000235:genetics
chemical_list: C105156:UBE2C protein, human; D044763:Ubiquitin-Conjugating Enzymes