title: Overexpression of cinnamate 4-hydroxylase gene enhances biosynthesis of decursinol angelate in Angelica gigas hairy roots.
abstract: Angelica gigas is a medicinal plant that produces pyranocoumarins, including decursin (D) and decursinol angelate (DA), which have neuroprotective, anticancer, and antiandrogenic effects. In this study, the coumarin biosynthetic pathway was engineered to increase the production of DA. Specifically, a vector was constructed which contained the A. gigas phenylalanine ammonia-lyase (AgPAL) and cinnamate 4-hydroxylase (AgC4H) genes that were driven by the cauliflower mosaic virus (CaMV) 35S promoter. Transgenic hairy roots that overexpressed AgPAL or AgC4H genes were obtained by using an Agrobacterium rhizogenes-mediated transformation system. Among them, only AgC4H-transgenic hairy root lines produced more DA than control transgenic hairy root lines. The enhanced gene expression corresponded to elevated C4H activities. This study showed the importance of C4H in the production of DA in A. gigas hairy root culture.
authors.affiliation: Department of Crop Science, Chungnam National University, 220 Gung-dong, Yuseong-gu, Daejeon 305-754, Korea.
authors.forename: Nam Il
authors.initials: NI
authors.lastname: Park
authors.forename: Jee Hee
authors.initials: JH
authors.lastname: Park
authors.forename: Sang Un
authors.initials: SU
authors.lastname: Park
mesh_terms: D060054:Agrobacterium / Q000235:genetics; D029969:Angelica / Q000235:genetics* / Q000378:metabolism*; D001578:Benzopyrans / Q000378:metabolism*; D002087:Butyrates / Q000378:metabolism*; D017796:Caulimovirus / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D010650:Phenylalanine Ammonia-Lyase / Q000235:genetics / Q000378:metabolism; D010936:Plant Extracts / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D050564:Trans-Cinnamate 4-Monooxygenase / Q000235:genetics* / Q000378:metabolism
chemical_list: D001578:Benzopyrans; D002087:Butyrates; D010936:Plant Extracts; C101278:decursin; D050564:Trans-Cinnamate 4-Monooxygenase; D010650:Phenylalanine Ammonia-Lyase