title: Determination of elaidic and vaccenic acids in foods using GC×GC-FID and GC×GC-TOFMS.
abstract: Trans fatty acids (TFAs) are present in meat and dairy products as m ruminant animals and in vegetable fats due to partial hydrogenation. This study aimed to discriminate between natural (N-TFA) and hydrogenated trans fatty (H-TFA) acids by GC×GC-flame ionization detection (GC×GC-FID) and comprehensive GC×GC-time-of-flight mass spectrometry (GC×GC-TOFMS). The separation of two kinds of trans fats, vaccenic acid (18:1 trans-11) and elaidic acid (18:1 trans-9), was performed using GC×GC-FID and GC×GC-TOFMS. A 100 m×0.25 mm I.D.×0.2 μm (film thickness) SP-2560 (bis-cyanopropyl polysiloxane) fused capillary column (first separation dimension, 1D) was coupled to a 1.5 m×0.18 mm I.D.×0.18 μm (film thickness) RTX-5 (5% diphenyl/95% dimethyl polysiloxane) fused capillary column (second separation dimension, 2D). The RSD of the intra-day repeatability by both GC×GC-FID and GC×GC-TOFMS for elaidic and vaccenic acids was ≤9.56% and ≤9.97%, and the RSD of the inter-day repeatability was ≤8.49 and ≤9.06%, respectively. It was found that the V/E value (vaccenic acid to elaidic acid ratio) could be used to distinguish H-TFA from N-TFA and to evaluate the quality of the fatty foods.
authors.affiliation: Food Analysis Center, Korea Food Research Institute, 516, Baekhyeon, Bundang, Seongnam, Gyeonggi 463-746, Republic of Korea. jhkfri@kfri.re.kr
authors.forename: Jaeho
authors.initials: J
authors.lastname: Ha
authors.forename: Dongwon
authors.initials: D
authors.lastname: Seo
authors.forename: Dongbin
authors.initials: D
authors.lastname: Shin
mesh_terms: D005504:Food Analysis / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D019301:Oleic Acid / Q000032:analysis*; D009829:Oleic Acids / Q000032:analysis*; D011786:Quality Control; D015203:Reproducibility of Results; D044242:Trans Fatty Acids / Q000032:analysis* / Q000592:standards
chemical_list: D009829:Oleic Acids; D044242:Trans Fatty Acids; D019301:Oleic Acid; C011459:elaidic acid; C050413:11-octadecenoic acid