title: Cloning, expression, purification, crystallization and preliminary X-ray crystallographic study of GK0767, the copper-containing nitrite reductase from Geobacillus kaustophilus.
abstract: The soluble region (residues 32-354) of GK0767, a copper-containing nitrite reductase from the thermophilic Gram-positive bacterium Geobacillus kaustophilus HTA426, has been cloned and overexpressed in Escherichia coli. The purified recombinant protein was crystallized using the hanging-drop vapour-diffusion method. X-ray diffraction data were collected and processed to a maximum resolution of 1.3 Å. The crystals belonged to space group R3, with unit-cell parameters a = b = 115.1, c = 87.5 Å. Preliminary studies and molecular-replacement calculations reveal the presence of one subunit of the homotrimeric structure in the asymmetric unit; this corresponds to a V(M) value of 3.14 Å(3) Da(-1).
authors.affiliation: Department of Chemistry, Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka, Japan.
authors.forename: Yohta
authors.initials: Y
authors.lastname: Fukuda
authors.forename: Taro
authors.initials: T
authors.lastname: Tamada
authors.forename: Hideto
authors.initials: H
authors.lastname: Takami
authors.forename: Shinnichiro
authors.initials: S
authors.lastname: Suzuki
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Inoue
authors.forename: Masaki
authors.initials: M
authors.lastname: Nojiri
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D015870:Gene Expression; D056506:Geobacillus / Q000201:enzymology*; D008969:Molecular Sequence Data; D009572:Nitrite Reductases / Q000737:chemistry* / Q000302:isolation & purification; D016415:Sequence Alignment
chemical_list: D009572:Nitrite Reductases; C052695:nitrite reductase, copper-containing