title: [The clinical significance of Claudin-7 and slug expression in lung squamous cell carcinoma and adenocarcinoma].
abstract: OBJECTIVE
  The aim of this study is to investigate the expression of Claudin-7, Slug and their correltion with clinicopathological characteristics in lung squamous cell carcinoma and adenocarcinoma. We also investigated the relationship between the two factors.
  
  
  METHODS
  The expressions of Claudin-7 and Slug in proteins were detected in 101 cases of lung squamous cell carcinoma and adenocarcinoma samples by immunohistochemistry SP method, and Western blot was applied to detect the expressions of Claudin-7 and Slug in 30 specimens of fresh lung cancer and corresponding paracancerous tissues.
  
  
  RESULTS
  The expression of Claudin-7 was remarkably decreased in squamous cell carcinoma and adenocarcinoma tissues compared with normal lung tissue, and its expression level was closely correlated with differentiation grade and lymphatic metastasis (P<0.05), whereas the expression of Slug was significantly higher in cancer tissues than that in normal lung tissue. Outside differentiation grade and lymphatic metastasis, the expression of Slug was related to TNM stage (P<0.05). The expression of Claudin-7 was negatively correlated with the expression of Slug in lung squamous cell carcinoma and adenocarcinoma (r=-0.566,8).
  
  
  CONCLUSIONS
  The down-regulation of Claudin-7 and overexpression of Slug might be one of pertinent biological markers for malignant transformation and metastasis of lung squamous cell carcinoma and adenocarcinoma.
authors.affiliation: Department of Pathology, China Medical University, and Liaoning Cancer Hospital, Shenyang 110001, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Li
authors.forename: Daorong
authors.initials: D
authors.lastname: Zhang
authors.forename: Cunwei
authors.initials: C
authors.lastname: Cai
authors.forename: Jingyu
authors.initials: J
authors.lastname: Dong
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D000077192:Adenocarcinoma of Lung; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D057167:Claudins; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D008875:Middle Aged; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: C473255:CLDN7 protein, human; D057167:Claudins; D008565:Membrane Proteins; C000604407:SNAI1 protein, human; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors