title: Comparison of 51chromium-labeled ethylenediamine tetra-acetic acid and iohexol as blood markers for intestinal permeability testing in Beagle dogs.
abstract: (51)Chromium-labeled ethylenediamine tetra-acetic acid ((51)Cr-EDTA) is the gold standard probe for assessing intestinal permeability (IP) in dogs, but exposure to radioactivity is a disadvantage. Iohexol is a safe contrast medium commonly used for medical imaging purposes and has been successfully applied more recently for the assessment of IP in animal models and humans. This study aimed at comparing (51)Cr-EDTA and iohexol as IP blood markers in dogs. A test solution containing (51)Cr-EDTA and iohexol was administered intragastrically to seven healthy laboratory Beagle dogs, and percentage recoveries in serum were calculated. The strong linear association (correlation, r=0.76 and linear regression, y=0.03+5.04x) between (51)Cr-EDTA and iohexol supports the potential usefulness of iohexol as an IP blood marker in dogs.
authors.affiliation: Central Animal Laboratory, University of Turku, Kiinamyllynkatu 10, C6, FIN-20520 Turku, Finland. raffri@utu.fi
authors.forename: Rafael
authors.initials: R
authors.lastname: Frias
authors.forename: Katrin
authors.initials: K
authors.lastname: Strube
authors.forename: Waldemar
authors.initials: W
authors.lastname: Ternes
authors.forename: M Carmen
authors.initials: MC
authors.lastname: Collado
authors.forename: Thomas
authors.initials: T
authors.lastname: Spillmann
authors.forename: Satu
authors.initials: S
authors.lastname: Sankari
authors.forename: Elias
authors.initials: E
authors.lastname: Westermarck
mesh_terms: D000818:Animals; D002857:Chromium / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics; D002860:Chromium Radioisotopes / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics; D003287:Contrast Media / Q000008:administration & dosage / Q000032:analysis / Q000493:pharmacokinetics*; D004283:Dog Diseases / Q000097:blood / Q000175:diagnosis* / Q000503:physiopathology; D004285:Dogs; D004338:Drug Combinations; D004492:Edetic Acid / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D005260:Female; D007410:Intestinal Diseases / Q000097:blood / Q000175:diagnosis / Q000503:physiopathology / Q000662:veterinary*; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000502:physiology*; D007472:Iohexol / Q000008:administration & dosage / Q000032:analysis / Q000493:pharmacokinetics*; D008297:Male; D010539:Permeability; D011849:Radioactive Tracers
chemical_list: D002860:Chromium Radioisotopes; D003287:Contrast Media; D004338:Drug Combinations; D011849:Radioactive Tracers; D002857:Chromium; D007472:Iohexol; D004492:Edetic Acid