title: [Usefulness of anti-SS-A/Ro antibody measurement based on fluorescence enzyme immunoassay with Ro60 and Ro52 antigen].
abstract: OBJECTIVE
  Anti-SS-A/Ro antibody is commonly found in the sera of patients with rheumatic disease. The antigenic components of SS-A/Ro are 60kD. and 52kD-Ro protein. Only anti-60kD SS-A/Ro antibody is detected by commonly used tests in clinical laboratories. "UniCAP EliA SS-A/Ro" is a new reagent using fluorescence enzyme immunoassay (FEIA) based on a mixture of both 60kD- and 52kD-Ro antigens. We evaluated the efficacy of detecting both anti-60kD and 52kD SS-A/Ro antibodies.
  
  
  METHODS
  We collected sera from 264 rheumatic disease patients and 106 healthy subjects. Anti-SS-A/Ro antibodies were measured by the new reagent along with conventional method. Anti-52kD and 60kD SS-A/Ro antibodies were measured by ELISA kit in rheumatic disease patients.
  
  
  RESULTS
  Anti-SS-A/Ro antibody levels were higher in patients with Sjögren's syndrome than those with SLE or RA. The prevalence of anti-SS-A/Ro antibody in rheumatic disease patients and healthy subjects were comparable with the conventional method, and patients with Sjögren's syndrome had highest prevalence of anti-SS-A/Ro antibody. Concordance rate between EliA and conventional method, and EliA and DID, were 96.6% and 94.3%, respectively. ELISA analysis revealed that patients with Sjögren's syndrome had anti-52kD SS-A/Ro antibody at high rates, while anti-60kD SS-A/Ro antibody was widely found in rheumatic disease patients. Three patients were positive only for anti-52kD SS-A/Ro antibody.
  
  
  CONCLUSIONS
  Taken together, "UniCAP EliA SS-A/Ro" is useful as a screening test for anti-SS-A/Ro antibodies.
authors.affiliation: The Center for Rheumatic Diseases, Shinko Hospital, Kobe 650-0072, Japan.
authors.forename: Noriko
authors.initials: N
authors.lastname: Fukuzumi
authors.forename: Nobuhide
authors.initials: N
authors.lastname: Hayashi
authors.forename: Jun
authors.initials: J
authors.lastname: Saegusa
authors.forename: Shouhiro
authors.initials: S
authors.lastname: Kinoshita
authors.forename: Seiji
authors.initials: S
authors.lastname: Kawano
authors.forename: Shunichi
authors.initials: S
authors.lastname: Kumagai
mesh_terms: D000328:Adult; D000974:Antibodies, Antinuclear / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015200:Fluoroimmunoassay / Q000379:methods*; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods*; D008297:Male; D008875:Middle Aged; D012372:ROC Curve; D011933:Reagent Kits, Diagnostic; D012216:Rheumatic Diseases / Q000175:diagnosis*; D012261:Ribonucleoproteins / Q000276:immunology*; D000099207:SS-A Antigen
chemical_list: D000974:Antibodies, Antinuclear; D015415:Biomarkers; D011933:Reagent Kits, Diagnostic; D012261:Ribonucleoproteins; D000099207:SS-A Antigen; C035356:SS-A antibodies