title: Roles of osmolality, calcium - Potassium antagonist and calcium in activation and flagellar beating pattern of sturgeon sperm.
abstract: The present study shows the roles of osmolality, calcium (Ca(2+))-potassium (K(+)) antagonist and Ca(2+) in sperm activation and flagellar beating of a sturgeon species, sterlet (Acipenser ruthenus). Sperm motility was activated at hypoosmolality relative to seminal plasma and suppressed at 175 mOsmol kg(-1). Sperm activation was totally suppressed by 0.35mM K(+), but Ca(2+) could fully reverse K(+) inhibitory effect at Ca(2+): K(+) ratio of 0.25. Neither EGTA (a chelator of Ca(2+) ions) nor nifedipine (a Ca(2+) channel blocker) prevented sperm activation. But, sperm motility and velocity were significantly decreased by EGTA, nifedipine and an inhibitor for Ca(2+)/calmodulin activated phosphodiesterase (w-7) that suggest role of Ca(2+) signaling after triggering sperm activation through hypoosmolality. Symmetric flagellar beating was also turned to asymmetric after activation in w-7, which is an evidence for modulation of Ca(2+)-binding proteins activity. Sturgeon sperm, similar to salmonids, is immotile in seminal plasma due to high K(+) concentrations, but the mechanism of sperm activation seems to be closer to other fish species where osmolality prohibits sperm activation in seminal plasma. In these species, hypoosmolality is the primary signal for sperm Ca(2+)-dependent signaling of axonemal beating.
authors.affiliation: University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters, South Bohemia Research Center of Aquaculture and Biodiversity of Hydrocenoses, Vodňany 389 25, Czech Republic. alavi@frov.jcu.cz
authors.forename: S M H
authors.initials: SM
authors.lastname: Alavi
authors.forename: D
authors.initials: D
authors.lastname: Gela
authors.forename: M
authors.initials: M
authors.lastname: Rodina
authors.forename: O
authors.initials: O
authors.lastname: Linhart
mesh_terms: D000818:Animals; D002118:Calcium / Q000494:pharmacology* / Q000502:physiology; D002121:Calcium Channel Blockers / Q000494:pharmacology; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000037:antagonists & inhibitors; D002614:Chelating Agents / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D029941:Fish Proteins / Q000037:antagonists & inhibitors; D005399:Fishes; D008297:Male; D009543:Nifedipine / Q000494:pharmacology; D009994:Osmolar Concentration; D011188:Potassium / Q000494:pharmacology* / Q000502:physiology; D013081:Sperm Motility / Q000187:drug effects*; D013082:Sperm Tail / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D013449:Sulfonamides / Q000494:pharmacology
chemical_list: D002121:Calcium Channel Blockers; D002614:Chelating Agents; D029941:Fish Proteins; D013449:Sulfonamides; D004533:Egtazic Acid; C017967:W 7; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D009543:Nifedipine; D011188:Potassium; D002118:Calcium