title: Reduction of enzyme dosage by oxygen delignification and mechanical refining for enzymatic hydrolysis of green liquor-pretreated hardwood.
abstract: In this study, a strategy to reduce enzyme dosage is evaluated by applying two post-treatments, oxygen delignification and mechanical refining. The sugar conversion for GL12 substrates was increased from 51.5% to 77.9% with post-treatments at the enzyme dosage of 10 FPU. When the amount of enzyme was reduced to 5 FPU with post-treatments, the conversion of 71.8% was obtained, which was significant higher than the conversion without any post-treatment using 10 FPU (51.5%). This clearly demonstrates the benefit of post-treatments that allows more than 50% of enzyme reduction at the same level of enzymatic conversion. Enzyme-accessible surface area and pore volume were evaluated by Simons' staining and DSC thermoporometry methods, and strong correlations were found with the sugar conversion.
authors.affiliation: Department of Forest Biomaterials, North Carolina State University, Raleigh, NC 27695, USA.
authors.forename: Bon-Wook
authors.initials: BW
authors.lastname: Koo
authors.forename: Trevor H
authors.initials: TH
authors.lastname: Treasure
authors.forename: Hasan
authors.initials: H
authors.lastname: Jameel
authors.forename: Richard B
authors.initials: RB
authors.lastname: Phillips
authors.forename: Hou-Min
authors.initials: HM
authors.lastname: Chang
authors.forename: Sunkyu
authors.initials: S
authors.lastname: Park
mesh_terms: D056804:Biofuels; D018533:Biomass; D019149:Bioreactors; D002254:Carbonates / Q000737:chemistry; D002480:Cellulase / Q000378:metabolism; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D006868:Hydrolysis; D007218:Industrial Microbiology / Q000379:methods*; D008031:Lignin / Q000378:metabolism*; D009005:Monosaccharides / Q000096:biosynthesis*; D010100:Oxygen; D016062:Porosity; D013440:Sulfides / Q000737:chemistry; D013696:Temperature; D014934:Wood / Q000378:metabolism*; D001617:beta-Glucosidase / Q000378:metabolism
chemical_list: D056804:Biofuels; D002254:Carbonates; D009005:Monosaccharides; D013440:Sulfides; C036909:lignocellulose; D000431:Ethanol; C005686:sodium carbonate; D008031:Lignin; D001617:beta-Glucosidase; D002480:Cellulase; D043364:Endo-1,4-beta Xylanases; D010100:Oxygen; C033479:sodium sulfide