title: Synaptic vesicle retrieval time is a cell-wide rather than individual-synapse property.
abstract: Although individual nerve terminals from the same neuron often differ in neurotransmitter release characteristics, the extent to which endocytic retrieval of synaptic vesicle components differs is unknown. We used high-fidelity optical recordings to undertake a large-scale analysis of endocytosis kinetics of individual boutons in hippocampal rat neurons. Our data indicate that endocytosis kinetics do not differ substantially across boutons from the same cell but instead appear to be controlled at a cell-wide level.
authors.affiliation: David Rockefeller Graduate Program of Rockefeller University, New York, New York, USA.
authors.forename: Moritz
authors.initials: M
authors.lastname: Armbruster
authors.forename: Timothy A
authors.initials: TA
authors.lastname: Ryan
mesh_terms: D018750:6-Cyano-7-nitroquinoxaline-2,3-dione / Q000494:pharmacology; D033962:Adaptor Protein Complex 2 / Q000378:metabolism; D000779:Anesthetics, Local / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn; D001703:Biophysics; D002478:Cells, Cultured; D004558:Electric Stimulation / Q000379:methods; D004705:Endocytosis / Q000187:drug effects / Q000502:physiology; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D049452:Green Fluorescent Proteins / Q000235:genetics; D006624:Hippocampus / Q000166:cytology; D008390:Markov Chains; D008959:Models, Neurological; D009474:Neurons / Q000166:cytology*; D017729:Presynaptic Terminals / Q000187:drug effects / Q000502:physiology*; D034741:RNA, Small Interfering / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013569:Synapses / Q000187:drug effects / Q000502:physiology*; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology; D013572:Synaptic Vesicles / Q000502:physiology*; D013779:Tetrodotoxin / Q000494:pharmacology; D013997:Time Factors; D014162:Transfection / Q000379:methods; D014633:Valine / Q000031:analogs & derivatives / Q000494:pharmacology; D050598:Vesicular Glutamate Transport Protein 1 / Q000235:genetics / Q000378:metabolism
chemical_list: D033962:Adaptor Protein Complex 2; D000779:Anesthetics, Local; D018691:Excitatory Amino Acid Antagonists; D034741:RNA, Small Interfering; C493465:Slc17a7 protein, mouse; D050598:Vesicular Glutamate Transport Protein 1; D049452:Green Fluorescent Proteins; D013779:Tetrodotoxin; D018750:6-Cyano-7-nitroquinoxaline-2,3-dione; C056639:2-amino-5-phosphopentanoic acid; D014633:Valine