title: EIN2 regulates salt stress response and interacts with a MA3 domain-containing protein ECIP1 in Arabidopsis.
abstract: Ethylene signalling regulates plant growth and development. However, its roles in salt stress response are less known. Here we studied functions of EIN2, a central membrane protein of ethylene signalling, and its interacting protein ECIP1 in salt stress responses. Mutation of EIN2 led to extreme salt sensitivity as revealed by phenotypic and physiological changes, and overexpression of C-terminus of EIN2 suppressed salt sensitivity in ein2-5, indicating that EIN2 is required for salt tolerance. Downstream components EIN3 and EIL1 are also essential for salt tolerance because ein3-1eil1-1 double mutant showed extreme salt-sensitive phenotype. A MA3 domain-containing protein ECIP1 was further identified to interact with EIN2 in yeast two-hybrid assay and GST pull-down assay. Loss-of-function of ECIP1 resulted in enhanced ethylene response but altered salt response during seed germination and plant growth. Double mutant analysis revealed that ein2-1 was epistatic to ecip1, and ecip1 mutation partially suppressed ethylene-insensitivity of etr2-1 and ein4-1. These studies strengthen that interactions between ECIP1 and EIN2 or ethylene receptors regulate ethylene response and stress response.
authors.affiliation: State Key Lab of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Lei
authors.forename: Ming
authors.initials: M
authors.lastname: Shen
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Li
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.forename: Kai-Xuan
authors.initials: KX
authors.lastname: Duan
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.forename: Yang-Rong
authors.initials: YR
authors.lastname: Cao
authors.forename: Wan-Ke
authors.initials: WK
authors.lastname: Zhang
authors.forename: Biao
authors.initials: B
authors.lastname: Ma
authors.forename: Hong-Qing
authors.initials: HQ
authors.lastname: Ling
authors.forename: Shou-Yi
authors.initials: SY
authors.lastname: Chen
authors.forename: Jin-Song
authors.initials: JS
authors.lastname: Zhang
mesh_terms: D020816:Amino Acid Motifs; D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000502:physiology* / Q000648:ultrastructure; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D018548:Cotyledon / Q000254:growth & development; D004843:Epistasis, Genetic; D005030:Ethylenes / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D018525:Germination; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D016254:Mutagenesis, Insertional; D010641:Phenotype; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D025941:Protein Interaction Mapping; D017434:Protein Structure, Tertiary; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism*; D055049:Salt Tolerance / Q000502:physiology*; D012639:Seeds / Q000502:physiology; D015398:Signal Transduction / Q000502:physiology*; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological; D020798:Two-Hybrid System Techniques
chemical_list: D029681:Arabidopsis Proteins; C582835:ECIP1 protein, Arabidopsis; C119963:EIN2 protein, Arabidopsis; C114576:EIN4 protein, Arabidopsis; C523313:ETR2 protein, Arabidopsis; D005030:Ethylenes; D047908:Intracellular Signaling Peptides and Proteins; D010940:Plant Proteins; D011956:Receptors, Cell Surface; C083750:ethylene receptors, plant; D012965:Sodium Chloride; C036216:ethylene