title: [Experimental food-induced fatty liver and its adjuvant therapy with natural bioactive substances].
abstract: Changes of redox-homeostasis generate cytokines, and free radicals influence many intracellular signaling pathways in different liver diseases. Liophylised table beet and carrot powder (GPS Powder Kft. 1361/004/2003BFÁÉÉÁ) containing bioactive components such as betaine, betanins, betaxanthins, flavonoids, polyphenols, glutamine, beta carotene, vitamins and folic acid may induce changes in various cellular pathways.
  
  
  OBJECTIVE
  The aim of this study was to determine the protecting effects of bioactive agents of the liophylised table beet and carrot powder on fatty liver in a "short term" experiment.
  
  
  METHODS
  Male Wistar rats were fed with chow with or without high fat (2% cholesterol, 0.5% cholic acid, 20% sunflower oil) and treated with 0.1 or 1 g/bwkg/day natural product for ten days parallel with the feedings. Cyclooxygenase-2, inducible nitric oxide synthase and tumor necrosis factor-α mRNA levels were determined using molecular biologic methods. Free radicals, H-donating activity, reducing power and free SH-group concentrations were determined by luminometry and spectrophotometry. Mobilized methyl groups were assayed by high pressure liquid chromatography method in liver homogenates.
  
  
  RESULTS
  It was found that the higher dose of the natural product better decreased the induced free radical reactions, cyclooxygenase-2, inducible nitric oxide synthase and tumor necrosis factor-α mRNA-levels both in normal and fatty liver tissues. Although treatments failed to exert significant changes in all global antioxidant parameters, mobilized methyl group concentrations were higher after treatments in fatty liver. Favorable tendencies were also noted in the redox-homeostasis of the fatty liver after treatment.
  
  
  CONCLUSIONS
  As expected, lyophylised table beet and carrot proved to be a "functional food" in rats with alimentary fat induced fatty liver. It cannot be ruled out that this beneficial effect may have clinical relevance.
authors.affiliation: Semmelweis Egyetem, Gyógyszerész-tudományi Kar, Farmakognóziai Intézet, Budapest. viktol19@yahoo.com
authors.forename: Viktor
authors.initials: V
authors.lastname: Hegedüs
authors.forename: Domokos
authors.initials: D
authors.lastname: Gerö
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Mihály
authors.forename: Attila
authors.initials: A
authors.lastname: Szijártó
authors.forename: Tivadar
authors.initials: T
authors.lastname: Zelles
authors.forename: Eva
authors.initials: E
authors.lastname: Sárdi
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage*; D027461:Beta vulgaris; D002851:Chromatography, High Pressure Liquid; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism*; D018552:Daucus carota; D004041:Dietary Fats / Q000008:administration & dosage; D005234:Fatty Liver / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism*; D005609:Free Radicals / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D049449:Luminescence; D008297:Male; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism*; D010084:Oxidation-Reduction; D011208:Powders; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013053:Spectrophotometry; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D004041:Dietary Fats; D005609:Free Radicals; D011208:Powders; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D052247:Nitric Oxide Synthase Type II; D051546:Cyclooxygenase 2