title: Human papillomavirus testing for suspected cervical cancer patients from Southern Assam by fast-PCR.
abstract: World-wide epidemiological studies have shown that cancer of the uterine cervix is the second most common malignant disease in women. Virtually every cervical cancer (99.7%) is HPV-positive, indicating that the presence of HPV is an obligatory element in their development. The present study was conducted by Fast-PCR (within 15 min.) based diagnosis of HPV 16 and HPV 18 infection amongst patients of suspected cervical cancer, confirmed by cytological methods. Twelve women, out of a total of fifty studied cases who had positive cervical pap smears (24%) were found to be positive for HPV 16/HPV 18 infection when PCR based technique was applied. The results indicate, perhaps, a greater specificity of PCR based diagnosis, or presence of other HPV subtypes as etiological factors in the present study group confined to Southern Assam.
authors.affiliation: Department of Biotechnology, Assam University, Assam, India. drsankarghosh@gmail.com
authors.forename: S K
authors.initials: SK
authors.lastname: Ghosh
authors.forename: B
authors.initials: B
authors.lastname: Choudhury
authors.forename: J
authors.initials: J
authors.lastname: Hansa
authors.forename: R
authors.initials: R
authors.lastname: Mondal
authors.forename: M
authors.initials: M
authors.lastname: Singh
authors.forename: M
authors.initials: M
authors.lastname: Singh
authors.forename: S
authors.initials: S
authors.lastname: Duttagupta
authors.forename: A
authors.initials: A
authors.lastname: Das
authors.forename: R
authors.initials: R
authors.lastname: Kumar
authors.forename: R S
authors.initials: RS
authors.lastname: Laskar
authors.forename: R
authors.initials: R
authors.lastname: Kannan
authors.forename: P R
authors.initials: PR
authors.lastname: Ghosh
mesh_terms: D000328:Adult; D000368:Aged; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D005500:Follow-Up Studies; D052162:Human papillomavirus 16 / Q000235:genetics* / Q000302:isolation & purification; D052161:Human papillomavirus 18 / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D007194:India; D008403:Mass Screening; D008875:Middle Aged; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000235:genetics / Q000821:virology; D016133:Polymerase Chain Reaction; D011379:Prognosis; D014412:Tumor Virus Infections / Q000175:diagnosis* / Q000235:genetics / Q000821:virology; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000821:virology; D002578:Uterine Cervical Dysplasia / Q000175:diagnosis* / Q000235:genetics / Q000821:virology
chemical_list: D004279:DNA, Viral