title: Detection of murine norovirus-1 by using TAT peptide-delivered molecular beacons.
abstract: A TAT peptide-delivered molecular beacon was developed and utilized to enumerate murine norovirus 1, a human norovirus (NoV) surrogate, in RAW 264.7 cells. This allowed the detection of a single infective virus within 6 h, a 12-fold improvement in time required for viral detection and quantification compared to that required by the conventional plaque assay.
authors.affiliation: Department of Environmental Sciences, University of California, Riverside, CA 92521, USA.
authors.forename: Payal S
authors.initials: PS
authors.lastname: Ganguli
authors.forename: Wilfred
authors.initials: W
authors.lastname: Chen
authors.forename: Marylynn V
authors.initials: MV
authors.lastname: Yates
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D005456:Fluorescent Dyes; D015696:Gene Products, tat; D051379:Mice; D029322:Norovirus / Q000235:genetics* / Q000302:isolation & purification*; D015345:Oligonucleotide Probes / Q000235:genetics; D012367:RNA, Viral
chemical_list: D005456:Fluorescent Dyes; D015696:Gene Products, tat; D015345:Oligonucleotide Probes; D012367:RNA, Viral