title: [Expression of nuclear factor kappaB and tumor necrosis factor α in lung tissue of paraquat poisoned rats].
abstract: OBJECTIVE
  To investigate the expression of nuclear factor kappa B (NF-kgr;B) and tumor necrosis factor α (TNF-α) in lung tissue of acute paraquat poisoned rats.
  
  
  METHODS
  68 male Wistar rats were randomly divided into 2 groups: the control group (n = 8), the intoxication group (n = 60). On the 1st, the 3rd, the 7th, the 14th and the 28th day after intoxication, the expression of NF-κB p65 and TNF-α in lung tissue were detected by LSAB immunohistochemistry (IH) staining. Meanwhile, the level of malondialdehyde (MDA) in plasma, and lung homogenate, the content of malondialdehyde (HPY) in lung homogenate were detected.
  
  
  RESULTS
  The levels of MDA in plasma on the 1st, the 3rd, the 7th day and in lung homogenate on the 1st, the 3rd day of the intoxication group [in plasma: (10.15 ± 3.15), (6.97 ± 1.65) and (5.44 ± 0.66) nmol/ml; in lung homogenate: (10.20 ± 2.43), (10.71 ± 171) nmol/ml] were significantly higher than that of the control group [in plasma: (3.84 ± 1.04) nmol/ml, in lung homogenate: (7.66 ± 0.66) nmol/ml]. The content of HPY in lung homogenate on the 14th and the 28th day after intoxication [(19.98 ± 2.86), (26.06 ± 4.06) µg/0.1 g lung homogenate] were higher than that of the control group [(8.80 ± 1.26) µg/0.1 g lung homogenate] significantly. The expression of NF-κB p65 and TNF-α in lung tissue were both significantly increased on the first day and the 3rd day of the intoxication group compared with the control group and weakened obviously after the 7th day.
  
  
  CONCLUSIONS
  Acute paraquat poisoning can induce increased expression of both NF-κB p65 and TNF-α in lung tissue; the enhanced activity of NF-κB may take part in the process of pulmonary injury in PQ poisoning.
authors.affiliation: Department of Emergency, Hebei Medical University, Shijiazhuang, China.
authors.forename: Jun-li
authors.initials: JL
authors.lastname: Hu
authors.forename: Han-wen
authors.initials: HW
authors.lastname: Shi
authors.forename: Ying-ping
authors.initials: YP
authors.lastname: Tian
authors.forename: Xia
authors.initials: X
authors.lastname: Wang
authors.forename: Heng-bo
authors.initials: HB
authors.lastname: Gao
authors.forename: Fei
authors.initials: F
authors.lastname: Tong
mesh_terms: D000818:Animals; D008168:Lung / Q000378:metabolism / Q000473:pathology; D008297:Male; D010269:Paraquat / Q000506:poisoning*; D051381:Rats; D017208:Rats, Wistar; D051996:Transcription Factor RelA / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: C498145:Rela protein, rat; D051996:Transcription Factor RelA; D014409:Tumor Necrosis Factor-alpha; D010269:Paraquat