title: [The expression and correlation of HMGB1 and VEGF protein in laryngeal squamous cell carcinoma].
abstract: OBJECTIVE
  To investigate the expression and biological significance of HMGB1 and VEGF protein in tissue specimens of laryngeal squamous cell carcinoma (LSCC), and further study the correlation between HMGB1 and VEGF protein.
  
  
  METHODS
  The expression of HMGB1 and VEGF protein was evaluated by immunohistochemical staining in 69 cases of LSCC specimens and 15 cases of adjacent epithelial tissue samples, and futher correlated with clinicopathologic parameters.
  
  
  RESULTS
  The positive rates of HMGB1 and VEGF in LSCC tissues were significantly higher than those in adjacent non-cancerous mucosa (P < 0.01), and the expression of these two marks was closely correlated with clinical stage (P < 0.05) and metastasis (P < 0.05) in LSCC. While the expression of HMGB1 and VEGF had no significant correlations with age, sex, histological differentiation and tumor site (P > 0. 05). There was a positive correlation between the expression of HMGB1 and VEGF (P < 0.05). The Kaplan-Meier survival analysis showed that patients with strong expression of HMGB1 or VEGF had poorer overall survival compared with that in patients with relative low HMGB1 or VEGF expression (P < 0.05). Multivariate COX regression analysis revealed that both lymph node metastasis and HMGB1 expression were independent prognostic factors for patients with LSCC.
  
  
  CONCLUSIONS
  This study demonstrated that HMGB1 and VEGF protein overexpression were closely associated with clinical stage, metastasis and poorer prognosis in patients with LSCC. Increased expression of these two proteins in LSCC suggested that HMGB1 and VEGF might play a critical role in the initiation and progression of LSCC.
authors.affiliation: Department of Otorhinolaryngology, Xiangya Hospital, Central South University, Changsha, 410008, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.forename: Yuanzhenk
authors.initials: Y
authors.lastname: Qiu
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Yongquan
authors.initials: Y
authors.lastname: Tian
authors.forename: Donghai
authors.initials: D
authors.lastname: Huang
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Zhou
authors.forename: Pingqing
authors.initials: P
authors.lastname: Tan
authors.forename: Changyun
authors.initials: C
authors.lastname: Yu
authors.forename: Lin
authors.initials: L
authors.lastname: Qi
authors.forename: Jianyun
authors.initials: J
authors.lastname: Xiao
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D005260:Female; D024243:HMGB1 Protein / Q000378:metabolism*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
chemical_list: D024243:HMGB1 Protein; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A