title: Acetylcholinesterase biosensor based on single-walled carbon nanotubes--Co phtalocyanine for organophosphorus pesticides detection.
abstract: A simple and reliable technique has been developed for the construction of an amperometric acetylcholinesterase biosensor based on screen-printed carbon electrodes. For the first time, one-step modification using single-walled carbon nanotubes and Co phtalocyanine has been proposed to decrease the working potential and to increase the signal of thiocholine oxidation. The biosensor developed made it possible to detect 5-50 ppb of paraoxon and 2-50 ppb of malaoxon with detection limits of 3 and 2 ppb, respectively (incubation 15 min). The biosensor showed high reproducibility when measurements of the substrate and inhibitor were performed (R.S.D. about 1% and 2.5%, respectively). The reliability of the inhibition measurements was confirmed by testing spiked samples of sparkling and tape waters.
authors.affiliation: Analytical Chemistry Department of Kazan Federal University, Kremlevskaya Street, 18, Kazan 420008, Russian Federation.
authors.forename: A N
authors.initials: AN
authors.lastname: Ivanov
authors.forename: R R
authors.initials: RR
authors.lastname: Younusov
authors.forename: G A
authors.initials: GA
authors.lastname: Evtugyn
authors.forename: F
authors.initials: F
authors.lastname: Arduini
authors.forename: D
authors.initials: D
authors.lastname: Moscone
authors.forename: G
authors.initials: G
authors.lastname: Palleschi
mesh_terms: D000110:Acetylcholinesterase; D015374:Biosensing Techniques / Q000379:methods*; D002800:Cholinesterase Inhibitors / Q000032:analysis*; D004566:Electrodes; D007211:Indoles; D054833:Isoindoles; D057230:Limit of Detection; D008294:Malathion / Q000031:analogs & derivatives / Q000032:analysis; D037742:Nanotubes, Carbon; D009943:Organophosphorus Compounds / Q000032:analysis; D010261:Paraoxon / Q000032:analysis; D010575:Pesticides / Q000032:analysis*; D015203:Reproducibility of Results
chemical_list: D002800:Cholinesterase Inhibitors; D007211:Indoles; D054833:Isoindoles; D037742:Nanotubes, Carbon; D009943:Organophosphorus Compounds; D010575:Pesticides; C100082:malaoxon; D000110:Acetylcholinesterase; D010261:Paraoxon; D008294:Malathion; C013647:phthalocyanine