title: Endomembrane Ca2+-ATPases play a significant role in virus-induced adaptation to oxidative stress.
abstract: Although the role of Ca2+ influx channels in oxidative stress signaling and cross-tolerance in plants is well established, little is known about the role of active Ca2+ efflux systems in this process. In our recent paper, we reported Potato Virus X (PVX)-induced acquired resistance to oxidative stress in Nicotiana benthamiana and showed the critical role of plasma membrane Ca2+/H+ exchangers in this process. The current study continues this research. Using biochemical and electrophysiological approaches, we reveal that both endomembrane P2A and P2B Ca2+-ATPases play significant roles in adaptive responses to oxidative stress by removing excessive Ca2+ from the cytosol, and that their functional expression is significantly altered in PVX-inoculated plants. These findings highlight the crucial role of Ca2+ efflux systems in acquired tolerance to oxidative stress and open up prospects for practical applications in agriculture, after in-depth comprehension of the fundamental mechanisms involved in common responses to environmental factors at the genomic, cellular and organismal levels.
authors.affiliation: School of Agricultural Science, University of Tasmania, Hobart, TAS, Australia. Sergey.Shabala@utas.edu.au
authors.forename: Sergey
authors.initials: S
authors.lastname: Shabala
authors.forename: Lone
authors.initials: L
authors.lastname: Bækgaard
authors.forename: Lana
authors.initials: L
authors.lastname: Shabala
authors.forename: Anja T
authors.initials: AT
authors.lastname: Fuglsang
authors.forename: Tracey A
authors.initials: TA
authors.lastname: Cuin
authors.forename: Lev G
authors.initials: LG
authors.lastname: Nemchinov
authors.forename: Michael G
authors.initials: MG
authors.lastname: Palmgren
mesh_terms: D002118:Calcium / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000235:genetics / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D018384:Oxidative Stress / Q000235:genetics / Q000502:physiology; D017863:Potexvirus / Q000502:physiology; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology*; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism* / Q000821:virology*
chemical_list: D000252:Calcium-Transporting ATPases; D002118:Calcium