title: Fast haptoglobin phenotyping based on microchip electrophoresis.
abstract: A new and fast method for haptoglobin phenotyping was developed based on microchip electrophoresis with laser induced fluorescence detection. Haptoglobin phenotypes 1-1 and 2-2 were labeled with fluorescein isothiocyanate. The analyses were performed on glass microchip which was simply treated with sodium dodecyl sulfate. After the optimization of the separation conditions, Hp 1-1 and Hp 2-2 could be differentiated in 150s and the detection limits for Hp 1-1 and Hp 2-2 were 0.39 and 0.62 μg/mL, respectively. Finally, the method was applied to human serum samples from healthy people and liver cancer patients. A decrease in Hp concentration for liver cancer patients was confirmed. Featuring high efficiency, speed, simplicity, the method reveals great potentials for the diagnosis of diseases and proteome research.
authors.affiliation: College of Chemistry, Beijing Normal University, Beijing 100875, China.
authors.forename: Bingrong
authors.initials: B
authors.lastname: Huang
authors.forename: Changgang
authors.initials: C
authors.lastname: Huang
authors.forename: Pingping
authors.initials: P
authors.lastname: Liu
authors.forename: Fangfang
authors.initials: F
authors.lastname: Wang
authors.forename: Na
authors.initials: N
authors.lastname: Na
authors.forename: Jin
authors.initials: J
authors.lastname: Ouyang
mesh_terms: D016022:Case-Control Studies; D046209:Electrophoresis, Microchip / Q000379:methods*; D019793:Fluorescein; D006242:Haptoglobins / Q000032:analysis*; D006801:Humans; D016130:Immunophenotyping / Q000379:methods*; D057230:Limit of Detection; D008113:Liver Neoplasms / Q000175:diagnosis*; D009363:Neoplasm Proteins / Q000097:blood*
chemical_list: D006242:Haptoglobins; D009363:Neoplasm Proteins; D019793:Fluorescein