title: Metabolic responses in gills of Manila clam Ruditapes philippinarum exposed to copper using NMR-based metabolomics.
abstract: Copper is an important heavy metal contaminant with high ecological risk in the Bohai Sea. In this study, the metabolic responses in the bioindicator, Manila clam (Ruditapes philippinarum), to the environmentally relevant copper exposures were characterized using NMR-based metabolomics. The significant metabolic changes corresponding to copper exposures were related to osmolytes, intermediates of the Krebs cycle and amino acids, such as the increase in homarine, branched chain amino acids and decrease in succinate, alanine and dimethylamine in the copper-exposed clam gills during 96 h exposure period. Overall, Cu may lead to the disturbances in osmotic regulation and energy metabolism in clams during 96 h experimental period. These results demonstrate that NMR-based metabolomics is applicable for the discovery of metabolic biomarkers which could be used to elucidate the toxicological mechanisms of marine heavy metal contaminants.
authors.affiliation: Key Laboratory of Coastal Zone Environment Processes, CAS, Shandong Provincial Key Laboratory of Coastal Zone Environment Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, PR China.
authors.forename: Linbao
authors.initials: L
authors.lastname: Zhang
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Liu
authors.forename: Liping
authors.initials: L
authors.lastname: You
authors.forename: Di
authors.initials: D
authors.lastname: Zhou
authors.forename: Huifeng
authors.initials: H
authors.lastname: Wu
authors.forename: Lianzhen
authors.initials: L
authors.lastname: Li
authors.forename: Jianmin
authors.initials: J
authors.lastname: Zhao
authors.forename: Jianghua
authors.initials: J
authors.lastname: Feng
authors.forename: Junbao
authors.initials: J
authors.lastname: Yu
mesh_terms: D000818:Animals; D049872:Bivalvia / Q000187:drug effects* / Q000378:metabolism; D003300:Copper / Q000633:toxicity*; D005786:Gene Expression Regulation / Q000187:drug effects; D005880:Gills / Q000187:drug effects* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D055432:Metabolomics; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D014874:Water Pollutants, Chemical; D003300:Copper