title: Protective effects and mechanism of tetramethylpyrazine against lens opacification induced by sodium selenite in rats.
abstract: Tetramethylpyrazine (TMP), extracted from the Chinese herbal medicine Ligusticum wallichii franchat (chuan xiong in Chinese), is a potent anti-free radical and calcium antagonist. Correspondingly, two important hypotheses in the causation of cataracts are free radical toxicity and calcium ion overload. In this study we investigated the effect of TMP on lens opacification induced by sodium selenite in rats, addressing the potential of TMP eye drops to prevent and treat cataracts. Results showed that the extent of lens opacification in the untreated Normal Control group (NC group) was significantly less than that of selenite-injected untreated rats (MC group) on days 3, 5, 7 and 10 (p < 0.001), while TMP treated selenite-injected rats (TMP group) had less lens opacification than the MC group on days 3, 5, 7 and 10 (p < 0.05). Compared with the NC group, the MC group had significantly decreased activity of super-oxide dismutase (SOD), glutathione peroxidase (GSH-PX) and catalase (CAT) and significantly elevated malondialdehyde (MDA) and calcium ion content (p < 0.001). Compared with the MC group, the activity of (SOD), (GSH-PX) and (CAT) were significantly higher while (MDA) and calcium ion levels were significantly lower in the TMP group at all time points (p < 0.01). The findings demonstrate that the selenite-induced cataract rat models were successfully built and the TMP eye drops can delay lens opacification induced by sodium selenite in rats. The mechanism by which TMP preserves lens transparency from selenite treated animals is associated with the lenses' ability to maintain normal levels of activity of SOD, GSH-PX and CAT and normal concentrations of MDA and calcium ion.
authors.affiliation: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, 54 Xianlie Road South, Guangzhou 510060, China.
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.forename: Yuexing
authors.initials: Y
authors.lastname: Zhu
authors.forename: Xinguo
authors.initials: X
authors.lastname: Deng
authors.forename: Yang
authors.initials: Y
authors.lastname: Gao
authors.forename: Yuguang
authors.initials: Y
authors.lastname: Zhu
authors.forename: Meifeng
authors.initials: M
authors.lastname: He
mesh_terms: D000287:Administration, Topical; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002121:Calcium Channel Blockers / Q000008:administration & dosage / Q000627:therapeutic use*; D002374:Catalase / Q000378:metabolism; D002386:Cataract / Q000139:chemically induced / Q000201:enzymology / Q000517:prevention & control*; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000627:therapeutic use*; D005979:Glutathione Peroxidase / Q000378:metabolism; D007908:Lens, Crystalline / Q000187:drug effects* / Q000378:metabolism; D030004:Ligusticum; D008315:Malondialdehyde / Q000378:metabolism; D010783:Photometry; D011719:Pyrazines / Q000008:administration & dosage / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018038:Sodium Selenite / Q000633:toxicity; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D002121:Calcium Channel Blockers; D004365:Drugs, Chinese Herbal; D011719:Pyrazines; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D018038:Sodium Selenite; D002118:Calcium; C017953:tetramethylpyrazine