title: Near-IR laser-triggered target cell collection using a carbon nanotube-based cell-cultured substrate.
abstract: Unique near-IR optical properties of single-walled carbon nanotube (SWNTs) are of interest in many biological applications. Here we describe the selective cell detachment and collection from an SWNT-coated cell-culture dish triggered by near-IR pulse laser irradiation. First, HeLa cells were cultured on an SWNT-coated dish prepared by a spraying of an aqueous SWNT dispersion on a glass dish. The SWNT-coated dish was found to show a good cell adhesion behavior as well as a cellular proliferation rate similar to a conventional glass dish. We discovered, by near-IR pulse laser irradiation (at the laser power over 25 mW) to the cell under optical microscopic observation, a quick single-cell detachment from the SWNT-coated surface. Shockwave generation from the irradiated SWNTs is expected to play an important role for the cell detachment. Moreover, we have succeeded in catapulting the target single cell from the cultured medium when the depth of the medium was below 150 μm and the laser power was stronger than 40 mW. The captured cell maintained its original shape. The retention of the genetic information of the cell was confirmed by the polymerase chain reaction (PCR) technique. A target single-cell collection from a culture medium under optical microscopic observation is significant in wide fields of single-cell studies in biological areas.
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
authors.forename: Takao
authors.initials: T
authors.lastname: Sada
authors.forename: Tsuyohiko
authors.initials: T
authors.lastname: Fujigaya
authors.forename: Yasuro
authors.initials: Y
authors.lastname: Niidome
authors.forename: Kohji
authors.initials: K
authors.lastname: Nakazawa
authors.forename: Naotoshi
authors.initials: N
authors.lastname: Nakashima
mesh_terms: D000327:Adsorption; D002478:Cells, Cultured; D006046:Gold / Q000737:chemistry; D006358:Hot Temperature; D006801:Humans; D007834:Lasers; D053768:Metal Nanoparticles / Q000737:chemistry; D008855:Microscopy, Electron, Scanning / Q000379:methods; D036103:Nanotechnology / Q000379:methods*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D011827:Radiation; D012709:Serum Albumin / Q000737:chemistry; D013055:Spectrophotometry, Infrared / Q000379:methods*; D013499:Surface Properties
chemical_list: D037742:Nanotubes, Carbon; D012709:Serum Albumin; D006046:Gold