title: Na+₋sensing quantum dots for cell-based screening of intracellular Na+ concentrations ([Na+]i).
abstract: We have developed a Na-quantum dot (QD) nanosensor for [Na(+)]i measurements. Using this Na-QD, we determined the dynamic physiological responses of [Na(+)]i in nonexcitable human HEK-293F cells and excitable primary rat cardiac myocytes by pharmacologically manipulating the membrane permeability to Na(+), the Na-K-2Cl cotransporter, and the Na(+)/H(+) antiporter. These data suggest that the mechanisms of [Na(+)]i homeostasis can now be elucidated with this novel Na-QD nanosensor. This could have a broad impact on Na(+) channel drug discovery.
authors.affiliation: Cytoptics Corporation, 9030 Kenamar Dr., Ste 308, San Diego, CA 92121, United States. ywang@cytoptics.com
authors.forename: Yuchi
authors.initials: Y
authors.lastname: Wang
authors.forename: Hua
authors.initials: H
authors.lastname: Mao
authors.forename: Lid B
authors.initials: LB
authors.lastname: Wong
mesh_terms: D000818:Animals; D002460:Cell Line; D004353:Drug Evaluation, Preclinical / Q000379:methods; D006706:Homeostasis; D006801:Humans; D032383:Myocytes, Cardiac / Q000378:metabolism; D045663:Quantum Dots; D051381:Rats; D012964:Sodium / Q000032:analysis* / Q000378:metabolism; D017923:Sodium-Hydrogen Exchangers / Q000502:physiology; D028021:Sodium-Potassium-Chloride Symporters / Q000502:physiology
chemical_list: D017923:Sodium-Hydrogen Exchangers; D028021:Sodium-Potassium-Chloride Symporters; D012964:Sodium