title: T-cell proliferation by surface molecules expression on polymorphonuclear neutrophils stimulated with IL-4 in superantigen presence.
abstract: BACKGROUND
  Polymorphonuclear neutrophils (PMNs) were originally described as short lived and terminally differentiated phagocytes that contribute only to the innate immune response. Some studies of PMNs cytokine production and expression of numerous cell surface proteins has suggested that PMNs are likely to influence adaptive responses and may satisfy the criteria of antigen presenting cells.
  
  
  OBJECTIVE
  This work aimed to study the effect of IL-4 in the function of PMNs as antigen presenting cells.
  
  
  METHODS
  Flow cytometry was used in the present study for the detection of cell surface human leukocyte antigen (HLA) class II, CD80 and CD86 required for antigen presentation and subsequent T-cell activation in the presence of Staphylococcus aureus enterotoxin (A). Human peripheral blood neutrophils were used for this purpose.
  
  
  RESULTS
  This study has shown that IL-4 stimulated PMNs for 24h expressed HLA class II, CD80 and CD86 that involved in antigen presentation. It also indicated that co-cultivation of IL-4 stimulated PMNs with autologous T-cells and in the presence of S. aureus enterotoxin (A) induced T-cell proliferation.
  
  
  CONCLUSIONS
  In vitro stimulation of PMNs with IL-4 showed expression of surface molecules involved in antigen presentation. In addition, the co-culture of T-Cells and stimulated PMNs showed high T-Cells proliferation in the presence of superantigens.
authors.affiliation: Department of Zoology, Minia University, El-Minia, Egypt. Manar_Muhamad@yahoo.com
authors.forename: B K A
authors.initials: BK
authors.lastname: Abdel-Salam
mesh_terms: D017951:Antigen Presentation; D018122:B7-1 Antigen / Q000378:metabolism; D051940:B7-2 Antigen / Q000378:metabolism; D049109:Cell Proliferation; D002469:Cell Separation; D002478:Cells, Cultured; D004768:Enterotoxins / Q000276:immunology / Q000378:metabolism*; D005434:Flow Cytometry; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D006801:Humans; D015847:Interleukin-4 / Q000276:immunology / Q000378:metabolism; D008213:Lymphocyte Activation; D009504:Neutrophils / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D018089:Superantigens / Q000276:immunology / Q000378:metabolism*; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism* / Q000473:pathology
chemical_list: D018122:B7-1 Antigen; D051940:B7-2 Antigen; D004768:Enterotoxins; D000949:Histocompatibility Antigens Class II; D018089:Superantigens; D015847:Interleukin-4; C026066:enterotoxin A, Staphylococcal