title: [Novel small compound in combination with cell-sheet technology for articular cartilage regeneration].
abstract: Aiming at regeneration of articular cartilage, we have established stable lines of mouse chondrogenic ATDC5 cells expressing green fluorescent protein under the control of type II collagen promoter fused with four repeats of a SOX9 enhancer (COL2A1-GFP) , as a monitoring system for chondrogenic differentiation. A screening of natural and synthetic compound libraries using the system identified some novel compounds. Combined with cell-sheet technology, a novel small compound was applied to the treatment of full-thickness knee cartilage defects in murine and canine models.
authors.affiliation: Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
authors.forename: Fumiko
authors.initials: F
authors.lastname: Yano
authors.forename: Hironori
authors.initials: H
authors.lastname: Hojo
authors.forename: Ung il
authors.initials: Ui
authors.lastname: Chung
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000502:physiology*; D002454:Cell Differentiation / Q000235:genetics; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology; D024043:Collagen Type II / Q000502:physiology; D004195:Disease Models, Animal; D004285:Dogs; D004353:Drug Evaluation, Preclinical; D049452:Green Fluorescent Proteins; D051379:Mice; D010118:Oxytetracycline / Q000494:pharmacology*; D012038:Regeneration / Q000187:drug effects; D055758:SOX9 Transcription Factor / Q000502:physiology; D023822:Tissue Engineering / Q000379:methods*
chemical_list: C447413:Col2a1 protein, mouse; D024043:Collagen Type II; D055758:SOX9 Transcription Factor; D049452:Green Fluorescent Proteins; D010118:Oxytetracycline