title: Mild detritylation of nucleic acid hydroxyl groups by warming up.
abstract: It is challenging to effectively deprotect hydroxyl groups of acid-or-base sensitive bio-macromolecules without causing even minor defects and compromising high quality of final products. We report here a mild detritylation strategy in mildly acidic buffers to remove the DMTr protection from the 5'-hydroxyl groups of synthetic nucleic acids. The DMTr-groups can be easily and effectively removed at pH 4.5 or 5.0 with slight warming up (40 °C), offering virtually quantitative deprotection. This warming-up strategy is particularly useful for deprotection of the modified nucleic acids that are sensitive to the conventional acid deprotection. As a first step towards our long-term goal of synthesizing defect-free nucleic acids, our novel and simple strategy further increases the quality of synthetic nucleic acids.
authors.affiliation: Department of Chemistry, Georgia State University, Atlanta, Georgia 30303, USA.
authors.forename: Jozef
authors.initials: J
authors.lastname: Salon
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Huang
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D006878:Hydroxides / Q000737:chemistry*; D012313:RNA / Q000737:chemistry* / Q000235:genetics; D014320:Triphenylmethyl Compounds / Q000737:chemistry*
chemical_list: D006878:Hydroxides; D014320:Triphenylmethyl Compounds; D012313:RNA; D004247:DNA; C031356:hydroxide ion