title: [Screening of high lipid production Trichosporon fermentans mutants by transposon tagging mTn-lacZ/leu2 insertion].
abstract: To improve microbial lipid production, we inserted mTn-lacZ/leu2 into Trichosporon fermentans 2.1368-Leu(-) to obtain high lipid production mutants. By observing the LacZ chromogenic change, the positive reaction between Cerulenin (inhibitor of fatty acid synthase) and phosphate vanillin, a higher lipid-producing mutant 2.1368-Leu(-)-7 grown on corn-stalk hydrolysate was obtained. The lipid content of this mutant reached 38.30% (8.97% higher than that of the control) and the lipid production rate was 8.35% (20.63% higher than that of the control). The rate of sugar utilization was 77%, meaning that 100 g corn-stalk could be converted to 8.32 g lipid. The study provided an effective method for microbial lipid production by using cheap raw materials for biodiesel.
authors.affiliation: Life Science College, Henan Agricultural University, Zhengzhou 450002, China. song1666@126.com
authors.forename: Andong
authors.initials: A
authors.lastname: Song
authors.forename: Yubo
authors.initials: Y
authors.lastname: Liu
authors.forename: Hui
authors.initials: H
authors.lastname: Xie
authors.forename: Fengqin
authors.initials: F
authors.lastname: Wang
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Bao
mesh_terms: D050539:3-Isopropylmalate Dehydrogenase / Q000235:genetics; D056804:Biofuels; D004251:DNA Transposable Elements / Q000235:genetics*; D005285:Fermentation; D007763:Lac Operon / Q000235:genetics*; D008055:Lipids / Q000096:biosynthesis*; D016254:Mutagenesis, Insertional; D009154:Mutation; D018547:Plant Stems / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics; D014250:Trichosporon / Q000235:genetics* / Q000378:metabolism; D003313:Zea mays / Q000378:metabolism
chemical_list: D056804:Biofuels; D004251:DNA Transposable Elements; D008055:Lipids; D029701:Saccharomyces cerevisiae Proteins; D050539:3-Isopropylmalate Dehydrogenase; C524993:LEU2 protein, S cerevisiae