title: Direct electrochemistry of horseradish peroxidase immobilized on electrografted 4-ethynylphenyl film via click chemistry.
abstract: In this paper, a simple two-step approach for redox protein immobilization was introduced. Firstly, alkynyl-terminated film was formed on electrode surface by electrochemical reduction of 4-ethylnylphenyl (4-EP) diazonium compound. Then, horseradish peroxidase (HRP) modified with azido group was covalently immobilized onto the electrografted film via click reaction. Reflection absorption infrared (RAIR) spectroscopy and electrochemical methods were used to characterize the modification process. The results indicate that HRP retains its native structure and shows fast direct electron transfer. Moreover, the immobilized HRP shows excellent electrocatalytic reduction activity toward H(2)O(2) with a linear range of 5.0×10(-6) to 9.3×10(-4) mol L(-1).
authors.affiliation: Department of Chemistry, East China Normal University, Shanghai, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Ran
authors.forename: Ru
authors.initials: R
authors.lastname: Peng
authors.forename: Cong
authors.initials: C
authors.lastname: Liang
authors.forename: Siqiu
authors.initials: S
authors.lastname: Ye
authors.forename: Yuezhong
authors.initials: Y
authors.lastname: Xian
authors.forename: Wenjing
authors.initials: W
authors.lastname: Zhang
authors.forename: Litong
authors.initials: L
authors.lastname: Jin
mesh_terms: D000327:Adsorption; D055162:Biocatalysis; D057930:Click Chemistry; D003979:Diazonium Compounds / Q000737:chemistry*; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D004579:Electron Transport; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D006046:Gold / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000737:chemistry* / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000032:analysis / Q000378:metabolism; D008958:Models, Molecular; D011487:Protein Conformation
chemical_list: C561694:4-ethylnylphenyl (4-EP) diazonium; D003979:Diazonium Compounds; D004800:Enzymes, Immobilized; D006046:Gold; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase