title: MG132 enhances neurite outgrowth in neurons overexpressing mutant TAR DNA-binding protein-43 via increase of HO-1.
abstract: In patients with amyotrophic lateral sclerosis (ALS), various mutations were identified in TAR DNA-binding protein-43 (TDP-43). In the present study, we found that mutant TDP-43 inhibited the neurite outgrowth. Subsequently, we tested the effect of MG132 on the mutant TDP-43 cell lines. Non-toxic doses of MG132 promoted neurite extension and decreased the level of thiobarbituric acid-reactive substances (TBARS). Heme oxygenase-1 (HO-1) known as antioxidase was restored by MG132. Conversely, Zinc protoporphyrin IX (ZnPP IX), which is an inhibitor of heme oxygenase, inhibited neurite outgrowth induced by MG132. It was well known that HO-1 was regulated by nuclear factor E2-related factor 2 (Nrf2). However, MG132 increased the expression of HO-1 independent of the Nrf2 pathway.
authors.affiliation: Department of Neurology, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, People's Republic of China.
authors.forename: Weisong
authors.initials: W
authors.lastname: Duan
authors.forename: Yansu
authors.initials: Y
authors.lastname: Guo
authors.forename: Hong
authors.initials: H
authors.lastname: Jiang
authors.forename: Xiujun
authors.initials: X
authors.lastname: Yu
authors.forename: Chunyan
authors.initials: C
authors.lastname: Li
mesh_terms: D015153:Blotting, Western; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology*; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics*; D049452:Green Fluorescent Proteins; D051547:Heme Oxygenase-1 / Q000096:biosynthesis*; D006801:Humans; D006822:Hybrid Cells; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007976:Leupeptins / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D009154:Mutation / Q000235:genetics / Q000502:physiology; D051267:NF-E2-Related Factor 2 / Q000378:metabolism; D016501:Neurites / Q000187:drug effects* / Q000378:metabolism; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D014162:Transfection
chemical_list: D015853:Cysteine Proteinase Inhibitors; D004268:DNA-Binding Proteins; D007976:Leupeptins; D051267:NF-E2-Related Factor 2; D049452:Green Fluorescent Proteins; D007770:L-Lactate Dehydrogenase; D051547:Heme Oxygenase-1; C072553:benzyloxycarbonylleucyl-leucyl-leucine aldehyde