title: Collagen levels are normalized after decompression of experimentally obstructed colon.
abstract: OBJECTIVE
  Our aim was to define the dynamics in collagen concentrations in the large bowel wall following decompression of experimental obstruction.
  
  
  METHODS
  Colonic obstruction was created in 28 male rats by the placement of a silicone ring around the distal colon. The ring was removed after 4 days to mimic endoscopical decompression by stent deployment. Colon circumference and collagen concentration were measured proximal to the obstructed segment immediately and at 3 and 10 days after decompression. The corresponding colonic sites of 23 sham-operated and eight nonoperated control animals were subjected to identical analyses.
  
  
  RESULTS
  Four days of obstruction resulted in a more than twofold increase in colonic circumference (20 vs 8 mm), with a concomitant 43% reduction (P = 0.001) in collagen concentration in the bowel wall proximal to the obstruction compared with sham animals. Three days after decompression, collagen concentrations remained reduced (P < 0.05), while there was no significant difference after 10 days with either sham-operated or nonoperated controls. Colonic circumference of the obstructed colon remained slightly distended (11 mm) on day 10 and tended to correlate (r(S) = 0.51, P = 0.053) with total matrix metalloproteinase activity.
  
  
  CONCLUSIONS
  The marked reduction in collagen concentration in an experimentally obstructed colon is normalized 10 days after decompression. These findings may have clinical implications for the timing of surgical resection.
authors.affiliation: Department of Surgery, Skåne University Hospital, Malmö, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Rehn
authors.forename: M S
authors.initials: MS
authors.lastname: Ågren
authors.forename: I
authors.initials: I
authors.lastname: Syk
mesh_terms: D000818:Animals; D003094:Collagen / Q000378:metabolism*; D003108:Colonic Diseases / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D019299:Decompression, Surgical; D007415:Intestinal Obstruction / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D008297:Male; D020782:Matrix Metalloproteinases / Q000378:metabolism; D023421:Models, Animal; D009929:Organ Size; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
chemical_list: D003094:Collagen; D020782:Matrix Metalloproteinases