title: Brevibacillus aydinogluensis sp. nov., a moderately thermophilic bacterium isolated from Karakoc hot spring.
abstract: Two Gram-positive, moderately thermophilic, endospore-forming, rod-shaped, motile bacteria, designated PDF25T and PDF30, were isolated from Karakoc hot spring in the province of Izmir, Turkey, and were characterized in order to determine their phylogenetic positions. 16S rRNA gene sequence analysis revealed that the two strains belonged to the genus Brevibacillus; strain PDF25T showed highest sequence similarity to strain PDF30 (99.4 %) and Brevibacillus thermoruber DSM 7064T (98.5 %). The major fatty acids of strain PDF25T were iso-C15:0 (39.30 %), anteiso-C15:0 (26.10 %) and iso-C16:0 (14.75 %). Polar lipid analysis revealed diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, and a variety of unidentified aminophospholipids, phospholipids and aminolipids. The major isoprenoid quinone was MK-7. The G+C content of the genomic DNA was 56.09 mol%. DNA-DNA hybridization experiments revealed 58 % relatedness between strain PDF25T and B. thermoruber DSM 7064T. Based on these data, the two strains are considered to represent a novel species of the genus Brevibacillus, for which the name Brevibacillus aydinogluensis sp. nov. is proposed. The type strain is PDF25T (=DSM 24395T=LMG 26289T).
authors.affiliation: Karadeniz Technical University, Faculty of Sciences, Department of Biology, 61080 Trabzon, Turkey.
authors.forename: Kadriye
authors.initials: K
authors.lastname: Inan
authors.affiliation: Karadeniz Technical University, Faculty of Sciences, Department of Biology, 61080 Trabzon, Turkey.
authors.forename: Sabriye
authors.initials: S
authors.lastname: Canakci
authors.affiliation: Karadeniz Technical University, Faculty of Sciences, Department of Biology, 61080 Trabzon, Turkey.
authors.forename: Ali Osman
authors.initials: AO
authors.lastname: Belduz
authors.affiliation: Yeditepe University, Faculty of Engineering and Architecture, Department of Genetics and Bioengineering, 34755 Istanbul, Turkey.
authors.forename: Fikrettin
authors.initials: F
authors.lastname: Sahin
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D058675:Brevibacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D045482:Hot Springs / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014421:Turkey; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C062629:menaquinone 7