title: Enhanced tolerance and remediation of anthracene by transgenic tobacco plants expressing a fungal glutathione transferase gene.
abstract: Plants can be used for remediation of polyaromatic hydrocarbons, which are known to be a major concern for human health. Metabolism of xenobiotic compounds in plants occurs in three phases and glutathione transferases (GST) mediate phase II of xenobiotic transformation. Plants, although have GSTs, they are not very efficient for degradation of exogenous recalcitrant xenobiotics including polyaromatic hydrocarbons. Hence, heterologous expression of efficient GSTs in plants may improve their remediation and degradation potential of xenobiotics. In the present study, we investigated the potential of transgenic tobacco plants expressing a Trichoderma virens GST for tolerance, remediation and degradation of anthracene-a recalcitrant polyaromatic hydrocarbon. Transgenic plants with fungal GST showed enhanced tolerance to anthracene compared to control plants. Remediation of (14)C uniformly labeled anthracene from solutions and soil by transgenic tobacco plants was higher compared to wild-type plants. Transgenic plants (T(0) and T(1)) degraded anthracene to naphthalene derivatives, while no such degradation was observed in wild-type plants. The present work has shown that in planta expression of a fungal GST in tobacco imparted enhanced tolerance as well as higher remediation potential of anthracene compared to wild-type plants.
authors.affiliation: Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Mumbai 400085, India.
authors.forename: Prachy
authors.initials: P
authors.lastname: Dixit
authors.forename: Prasun K
authors.initials: PK
authors.lastname: Mukherjee
authors.forename: Pramod D
authors.initials: PD
authors.lastname: Sherkhane
authors.forename: Sharad P
authors.initials: SP
authors.lastname: Kale
authors.forename: Susan
authors.initials: S
authors.lastname: Eapen
mesh_terms: D000873:Anthracenes / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D052918:Environmental Restoration and Remediation / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry; D005800:Genes, Fungal; D005982:Glutathione Transferase / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D014026:Nicotiana / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D014242:Trichoderma / Q000235:genetics*
chemical_list: D000873:Anthracenes; D005982:Glutathione Transferase