title: Proteomic analysis of microvesicles derived from human colorectal cancer ascites.
abstract: The presence of malignant ascites in the peritoneal cavity is a poor prognostic indicator of low survival rate. Various cancer cells, including those of colorectal cancer (CRC), release microvesicles (exosomes) into surrounding tissues and peripheral circulation including malignant ascites. Although recent progress has revealed that microvesicles play multiple roles in tumor progression, the protein composition and the pathological function of malignant ascites-derived microvesicles are still unknown. Here, we report the first global proteomic analyses of highly purified microvesicles derived from human CRC ascites. With 1-D SDS-PAGE and nano-LC-MS/MS analyses, we identified a total of 846 microvesicular proteins from ascites of three CRC patients with high confidence; 384 proteins were identified in at least two patients. We identified proteins that might function in tumor progression via disruption of epithelial polarity, migration, invasion, tumor growth, immune modulation, and angiogenesis. Furthermore, we identified several potential diagnostic markers of CRC including colon-specific surface antigens. Our proteomic analyses will help to elucidate diverse functions of microvesicles in cancer progression and will aid in the development of novel diagnostic tools for CRC.
authors.affiliation: Department of Life Science and Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang, Republic of Korea.
authors.forename: Dong-Sic
authors.initials: DS
authors.lastname: Choi
authors.forename: Jung Ok
authors.initials: JO
authors.lastname: Park
authors.forename: Su Chul
authors.initials: SC
authors.lastname: Jang
authors.forename: Yae Jin
authors.initials: YJ
authors.lastname: Yoon
authors.forename: Jin Woo
authors.initials: JW
authors.lastname: Jung
authors.forename: Do-Young
authors.initials: DY
authors.lastname: Choi
authors.forename: Jung-Wook
authors.initials: JW
authors.lastname: Kim
authors.forename: Ji Seon
authors.initials: JS
authors.lastname: Kang
authors.forename: Jaesung
authors.initials: J
authors.lastname: Park
authors.forename: Daehee
authors.initials: D
authors.lastname: Hwang
authors.forename: Kyung-Hee
authors.initials: KH
authors.lastname: Lee
authors.forename: Sang-Hyun
authors.initials: SH
authors.lastname: Park
authors.forename: Yoon-Keun
authors.initials: YK
authors.lastname: Kim
authors.forename: Dominic M
authors.initials: DM
authors.lastname: Desiderio
authors.forename: Kwang Pyo
authors.initials: KP
authors.lastname: Kim
authors.forename: Yong Song
authors.initials: YS
authors.lastname: Gho
mesh_terms: D001201:Ascites / Q000473:pathology*; D015179:Colorectal Neoplasms / Q000473:pathology*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D055354:Exosomes / Q000737:chemistry*; D006801:Humans; D009363:Neoplasm Proteins / Q000032:analysis*; D020543:Proteome / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D009363:Neoplasm Proteins; D020543:Proteome