title: [The characteristics of type I, III collagen and LN in pulmonary fibrosis induced by uranium ore dust in rats].
abstract: OBJECTIVE
  To explore the characteristics of LN and type I, III collagen in pulmonary fibrosis induced by uranium ore dust in rats.
  
  
  METHODS
  60 adult Wistar rats were divided randomly into two groups, control group (30 rats) and uranium ore dust group (30 rats). Non-exposed intratracheal instillation method was used. Uranium ore dust group was exposed 20 mg/ml uranium ore dust suspension 1ml per rat, meanwhile control group was exposed normal saline 1ml per rat. Post-exposed the 7, 14, 21, 30 and 60 d, 6 rats in each group were killed randomly, lung tissue were collected. The pathological changes in lung tissue were observed by microscope using HE staining, the collagen I and III in lungs were observed by polarizing microscope using Biebrich scarlet staining. The expression of LN protein in lung tissue was observed by immunohistochemistry-SP.
  
  
  RESULTS
  During lung fibrosis, a large amount of the proliferated I and III collagen in lungs were observed. Post-exposure to uranium ore dust, the characteristics in proliferated collagen in lungs were type I collagen deposited in lung interstitium mainly in the early stage. The area percentage of collagen I and III was increased significantly at 7, 14, 21, 30 and 60d in the experimental group as compared with that in the control group (P < 0.05 or P < 0.01). The over expression of LN in the lung tissue were observed. The expression of LN was distributed in the lung tissue as thickening of the linear or cluster. The integral optical density of LN was increased significantly at 21, 30 and 60 d in the experimental group as compared with that in the control group (P < 0.05 or P < 0.01).
  
  
  CONCLUSIONS
  After exposure to uranium ore dust, the characteristics in proliferated collagen in lungs are the type of I collagen deposited in lung interstitium mainly in the early stage, while the type of III collagen increase significantly at the later period. The overexpression of LN exists in the process of pulmonary fibrosis. It suggests that LN has a role effect in the process of pulmonary fibrosis.
authors.affiliation: Department of Occupational and Environmental Hygiene, University of South China, Hengyang, Hunan, 421001, China.
authors.forename: Ying-chun
authors.initials: YC
authors.lastname: Hu
authors.forename: Zhen-hua
authors.initials: ZH
authors.lastname: Luo
authors.forename: Xing-jiang
authors.initials: XJ
authors.lastname: Yuan
authors.forename: Li-ping
authors.initials: LP
authors.lastname: Yang
authors.forename: Shou-feng
authors.initials: SF
authors.lastname: Wang
authors.forename: Guang-yue
authors.initials: GY
authors.lastname: Li
authors.forename: Xing-peng
authors.initials: XP
authors.lastname: He
mesh_terms: D000818:Animals; D024042:Collagen Type I / Q000378:metabolism*; D024061:Collagen Type III / Q000378:metabolism*; D004391:Dust; D005260:Female; D007797:Laminin / Q000378:metabolism*; D008297:Male; D011658:Pulmonary Fibrosis / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D014501:Uranium / Q000009:adverse effects*
chemical_list: D024042:Collagen Type I; D024061:Collagen Type III; D004391:Dust; D007797:Laminin; D014501:Uranium