title: Construction and structural analysis of tethered lipid bilayer containing photosynthetic antenna proteins for functional analysis.
abstract: The construction and structural analysis of a tethered planar lipid bilayer containing bacterial photosynthetic membrane proteins, light-harvesting complex 2 (LH2), and light-harvesting core complex (LH1-RC) is described and establishes this system as an experimental platform for their functional analysis. The planar lipid bilayer containing LH2 and/or LH1-RC complexes was successfully formed on an avidin-immobilized coverglass via an avidin-biotin linkage. Atomic force microscopy (AFM) showed that a smooth continuous membrane was formed there. Lateral diffusion of these membrane proteins, observed by a fluorescence recovery after photobleaching (FRAP), is discussed in terms of the membrane architecture. Energy transfer from LH2 to LH1-RC within the tethered membrane was observed by steady-state fluorescence spectroscopy, indicating that the tethered membrane can mimic the natural situation.
authors.affiliation: Department of Frontier Materials, Graduate School of Engineering, Nagoya Institute of Technology, Gokiso-cho, Showa-ku, Nagoya 466-8555, Japan.
authors.forename: Ayumi
authors.initials: A
authors.lastname: Sumino
authors.forename: Takehisa
authors.initials: T
authors.lastname: Dewa
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Takeuchi
authors.forename: Ryuta
authors.initials: R
authors.lastname: Sugiura
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Sasaki
authors.forename: Nobuo
authors.initials: N
authors.lastname: Misawa
authors.forename: Ryugo
authors.initials: R
authors.lastname: Tero
authors.forename: Tsuneo
authors.initials: T
authors.lastname: Urisu
authors.forename: Alastair T
authors.initials: AT
authors.lastname: Gardiner
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Cogdell
authors.forename: Hideki
authors.initials: H
authors.lastname: Hashimoto
authors.forename: Mamoru
authors.initials: M
authors.lastname: Nango
mesh_terms: D045342:Light-Harvesting Protein Complexes / Q000737:chemistry / Q000378:metabolism*; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D008968:Molecular Conformation; D010316:Particle Size; D013499:Surface Properties
chemical_list: D045342:Light-Harvesting Protein Complexes; D008051:Lipid Bilayers