title: Native multimer analysis of plasma and platelet von Willebrand factor compared to denaturing separation: implication for the interpretation of satellite bands.
abstract: Blue native electrophoresis (BNE) was applied to analyze the von Willebrand factor (vWF) multimers in their native state and to present a methodology to perform blue native electrophoresis on human plasma proteins, which has not been done before. The major difference between this method and the commonly used SDS-agarose gel electrophoresis is the lack of satellite bands in the high-resolution native gel. To further analyze this phenomenon, a second dimension was performed under denaturing conditions. Thereby, we obtained a pattern in which each protein sub-unit from the first dimension dissociates into three distinct sub-bands. These bands confirm the triplet structure, which consists of an intermediate band and two satellite bands. By introducing the second dimension, our novel method separates the triplet structure into a higher resolution than the commonly used SDS-agarose gel electrophoresis does. This helps considerably in the classification of ambiguous von Willebrand's disease subtypes. In addition, our method has the additional advantage of being able to resolve the triplet structure of platelet vWF multimers, which has not been identified previously through conventional SDS-agarose electrophoresis multimer analysis. This potential enables us to compare the triplet structure from platelet and plasmatic vWF, and may help to find out whether structural abnormalities concern the vWF molecule in the platelet itself, or whether they are due to the physiological processing of vWF shed into circulation. Owing to its resolution and sensitivity, this native separation technique offers a promising tool for the analysis and detection of von Willebrand disorder, and for the classification of von Willebrand's disease subtypes.
authors.affiliation: Institute of Medical and Chemical Laboratory Diagnostics (ZIMCL), University Hospital, Innsbruck, Austria.
authors.forename: Kurt
authors.initials: K
authors.lastname: Hohenstein
authors.forename: Andrea
authors.initials: A
authors.lastname: Griesmacher
authors.forename: Günter
authors.initials: G
authors.lastname: Weigel
authors.forename: Georg
authors.initials: G
authors.lastname: Golderer
authors.forename: Helmut Werner
authors.initials: HW
authors.lastname: Ott
mesh_terms: D001792:Blood Platelets / Q000737:chemistry*; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D046912:Multiprotein Complexes / Q000032:analysis / Q000737:chemistry*; D011489:Protein Denaturation; D021122:Protein Subunits; D013997:Time Factors; D014842:von Willebrand Diseases / Q000097:blood / Q000145:classification*; D014841:von Willebrand Factor / Q000032:analysis / Q000737:chemistry*
chemical_list: D046912:Multiprotein Complexes; D021122:Protein Subunits; D014841:von Willebrand Factor