title: Highly sensitive detection of clenbuterol using competitive surface-enhanced Raman scattering immunoassay.
abstract: In this report, we present a novel approach to detect clenbuterol based on competitive surface-enhanced Raman scattering (SERS) immunoassay. Herein, a SERS nanoprobe that relies on gold nanoparticle (GNP) is labeled by 4,4'-dipyridyl (DP) and clenbuterol antibody, respectively. The detection of clenbuterol is carried out by competitive binding between free clenbuterol and clenbuterol-BSA fastened on the substrate with their antibody labeled on SERS nanoprobes. The present method allows us to detect clenbuterol over a much wider concentration range (0.1-100 pg mL(-1)) with a lower limit of detection (ca. 0.1 pg mL(-1)) than the conventional methods. Furthermore, by the use of this new competitive SERS immunoassay, the clenbuterol-BSA (antigen) is chosen to fasten on the substrate instead of the clenbuterol antibody, which could reduce the cost of the assay. Results demonstrate that the proposed method has the wide potential applications in food safety and agonist control.
authors.affiliation: MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, China.
authors.forename: Guichi
authors.initials: G
authors.lastname: Zhu
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Hu
authors.forename: Jiao
authors.initials: J
authors.lastname: Gao
authors.forename: Liang
authors.initials: L
authors.lastname: Zhong
mesh_terms: D058666:Adrenergic beta-2 Receptor Agonists / Q000276:immunology / Q000652:urine*; D000818:Animals; D001667:Binding, Competitive; D002417:Cattle; D002976:Clenbuterol / Q000276:immunology / Q000652:urine*; D005506:Food Contamination / Q000517:prevention & control; D059022:Food Safety; D006046:Gold / Q000737:chemistry; D007118:Immunoassay / Q000379:methods*; D053768:Metal Nanoparticles / Q000737:chemistry; D011725:Pyridines / Q000737:chemistry; D013499:Surface Properties; D013552:Swine / Q000652:urine
chemical_list: D058666:Adrenergic beta-2 Receptor Agonists; D011725:Pyridines; D006046:Gold; C034306:4,4'-bipyridyl; D002976:Clenbuterol