title: Enhancement of Astragalus polysaccharide on the immune responses in pigs inoculated with foot-and-mouth disease virus vaccine.
abstract: The effects of Astragalus polysaccharides (APS) on the immune response in pigs immunized with foot-and-mouth disease virus (FMDV) vaccine were investigated. Fifteen pigs were randomly divided into five groups. Four groups were vaccinated with a FMDV inactivated vaccine. Pigs in three experimental groups were administered varying doses of APS (APS1, 5mg/kg; APS2, 10mg/kg; APS3, 20mg/kg). The influence of APS on the number of CD3(+)CD4(-)CD8(+) cytotoxic T cells, CD3(+)CD4(+)CD8(+) T helper memory cells, and CD3(-)CD4(-)CD8(+) natural killer cells among peripheral blood lymphocytes (PBL) in the three APS groups were significant compared to the vaccine group. In vitro stimulation of PBL by Con A and LPS in APS groups induced a stronger proliferative response at 2 and 6 weeks post-inoculation (PI). APS markedly increased the titer of FMDV-specific antibody in a dose-dependent manner, and up-regulated mRNA expression of IFN-γ and IL-6. APS could potentially be used as an immunomodulator for a FMDV vaccine and provide better protection against FMDV.
authors.affiliation: College of Veterinary Medicine, China Agricultural University, Beijing 100193, People's Republic of China.
authors.forename: Jiefeng
authors.initials: J
authors.lastname: Li
authors.forename: Yougang
authors.initials: Y
authors.lastname: Zhong
authors.forename: Huanrong
authors.initials: H
authors.lastname: Li
authors.forename: Nuowei
authors.initials: N
authors.lastname: Zhang
authors.forename: Wuren
authors.initials: W
authors.lastname: Ma
authors.forename: Guilin
authors.initials: G
authors.lastname: Cheng
authors.forename: Faqiang
authors.initials: F
authors.lastname: Liu
authors.forename: Fenghua
authors.initials: F
authors.lastname: Liu
authors.forename: Jianqin
authors.initials: J
authors.lastname: Xu
mesh_terms: D000818:Animals; D000906:Antibodies / Q000097:blood; D027883:Astragalus Plant / Q000737:chemistry*; D049109:Cell Proliferation / Q000187:drug effects; D005536:Foot-and-Mouth Disease / Q000276:immunology*; D029544:Foot-and-Mouth Disease Virus / Q000276:immunology*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000276:immunology; D007155:Immunologic Factors / Q000494:pharmacology*; D007371:Interferon-gamma / Q000235:genetics; D015850:Interleukin-6 / Q000235:genetics; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D011134:Polysaccharides / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013552:Swine; D014611:Vaccination; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000906:Antibodies; D007155:Immunologic Factors; D015850:Interleukin-6; D011134:Polysaccharides; D012333:RNA, Messenger; D014765:Viral Vaccines; D007371:Interferon-gamma