title: Mechanism of lipid extraction from Botryococcus braunii FACHB 357 in a biphasic bioreactor.
abstract: Algal lipid of Botryococcus braunii could be produced continuously and in situ extracted in an aqueous-organic bioreactor. In this study, the cell ultra-structure and cell membrane permeability of B. braunii FACHB 357 were investigated to understand the mechanism of lipid extraction within the biphasic system. The results showed that biocompatible solvent of tetradecane could induce algal lipid accumulation, enable the cell membrane more active and the cell wall much looser. The exocytosis process was observed to be one of the mechanisms for lipid cross-membrane extraction in the presence of organic solvent.
authors.affiliation: Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027, PR China.
authors.forename: Fang
authors.initials: F
authors.lastname: Zhang
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Cheng
authors.forename: Wang-Lei
authors.initials: WL
authors.lastname: Gao
authors.forename: Xin-Hua
authors.initials: XH
authors.lastname: Xu
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.forename: Huan-Lin
authors.initials: HL
authors.lastname: Chen
mesh_terms: D019149:Bioreactors; D000460:Chlorophyta / Q000737:chemistry*; D008055:Lipids / Q000302:isolation & purification*
chemical_list: D008055:Lipids