title: RYH: a minimal peptidic sequence obtained from beta-chain hemoglobin exhibiting an antimicrobial activity.
abstract: Bovine hemoglobin is an animal protein described as source of bioactive peptides. Enzymatic hydrolysis of this protein results into some peptides exhibiting antimicrobial activity against Gram-positive and Gram-negative bacteria. In this study, a family of peptides from the beta chain (beta-114-145 derived peptides) obtained by peptic hydrolysis of bovine hemoglobin, was purified by reverse-phase HPLC and characterized by different analytical techniques (mass spectrometry, circular dichroism). The minimum inhibitory concentration was determined to show the antimicrobial activity of these peptides. Four bacterial strains were used: two Gram-negative (Escherichia coli and Salmonella Enteritidis) and two Gram-positive strains (Listeria innocua and Micrococcus luteus). The effect of these peptides on artificial membrane was also measured. Our findings showed that the peptide β114-145 and its peptic derivatives contain the RYH sequence. The most antimicrobial peptide is the RYH peptide which was the shortest one.
authors.affiliation: Laboratoire ProBioGEM, Bâtiment Polytech-Lille, Boulevard Paul Langevin, 59655 Villeneuve d'Ascq, France.
authors.forename: Lucie
authors.initials: L
authors.lastname: Catiau
authors.forename: Johnatan
authors.initials: J
authors.lastname: Traisnel
authors.forename: Nour-Eddine
authors.initials: NE
authors.lastname: Chihib
authors.forename: Guillaume
authors.initials: G
authors.lastname: Le Flem
authors.forename: Annick
authors.initials: A
authors.lastname: Blanpain
authors.forename: Oleg
authors.initials: O
authors.lastname: Melnyk
authors.forename: Didier
authors.initials: D
authors.lastname: Guillochon
authors.forename: Naïma
authors.initials: N
authors.lastname: Nedjar-Arroume
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D005452:Fluoresceins / Q000032:analysis; D006090:Gram-Negative Bacteria / Q000187:drug effects* / Q000254:growth & development; D006094:Gram-Positive Bacteria / Q000187:drug effects* / Q000254:growth & development; D006454:Hemoglobins / Q000737:chemistry*; D006868:Hydrolysis; D008081:Liposomes / Q000378:metabolism; D013058:Mass Spectrometry; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D005452:Fluoresceins; D006454:Hemoglobins; D008081:Liposomes; D010446:Peptide Fragments; C024098:6-carboxyfluorescein