title: Effects of canola and corn oil mimetic on Jurkat cells.
abstract: BACKGROUND
  The Western diet is high in omega-6 fatty acids and low in omega-3 fatty acids. Canola oil contains a healthier omega 3 to omega 6 ratio than corn oil. Jurkat T leukemia cells were treated with free fatty acids mixtures in ratios mimicking that found in commercially available canola oil (7% α-linolenic, 30% linoleic, 54% oleic) or corn oil (59% linoleic, 24% oleic) to determine the cell survival or cell death and changes in expression levels of inflammatory cytokines and receptors following oil treatment.
  
  
  METHODS
  Fatty acid uptake was assessed by gas chromatography. Cell survival and cell death were evaluated by cell cycle analyses, propidium-iodide staining, trypan blue exclusion and phosphatidylserine externalization. mRNA levels of inflammatory cytokines and receptors were assessed by RT-PCR.
  
  
  RESULTS
  There was a significant difference in the lipid profiles of the cells after treatment. Differential action of the oils on inflammatory molecules, following treatment at non-cytotoxic levels, indicated that canola oil mimetic was anti-inflammatory whereas corn oil mimetic was pro-inflammatory.
  
  
  CONCLUSIONS
  These results indicate that use of canola oil in the diet instead of corn oil might be beneficial for diseases promoted by inflammation.
authors.affiliation: Department of Biochemistry and Microbiology, Marshall University School of Medicine, Huntington, WV, USA. ion@marshall.edu
authors.forename: Gabriela
authors.initials: G
authors.lastname: Ion
authors.forename: Kayla
authors.initials: K
authors.lastname: Fazio
authors.forename: Juliana A
authors.initials: JA
authors.lastname: Akinsete
authors.forename: W Elaine
authors.initials: WE
authors.lastname: Hardman
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D003314:Corn Oil / Q000737:chemistry / Q000494:pharmacology*; D016207:Cytokines / Q000378:metabolism; D053938:DNA Fragmentation / Q000187:drug effects; D005229:Fatty Acids, Monounsaturated / Q000737:chemistry / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism; D019169:Jurkat Cells; D008055:Lipids / Q000032:analysis; D000074262:Rapeseed Oil; D018121:Receptors, Cytokine / Q000378:metabolism
chemical_list: D016207:Cytokines; D005229:Fatty Acids, Monounsaturated; D018836:Inflammation Mediators; D008055:Lipids; D000074262:Rapeseed Oil; D018121:Receptors, Cytokine; D003314:Corn Oil