title: Structural and functional relationships between the lectin and arm domains of calreticulin.
abstract: Calreticulin and calnexin are key components in maintaining the quality control of glycoprotein folding within the endoplasmic reticulum. Although their lectin function of binding monoglucosylated sugar moieties of glycoproteins is well documented, their chaperone activity in suppressing protein aggregation is less well understood. Here, we use a series of deletion mutants of calreticulin to demonstrate that its aggregation suppression function resides primarily within its lectin domain. Using hydrophobic peptides as substrate mimetics, we show that aggregation suppression is mediated through a single polypeptide binding site that exhibits a K(d) for peptides of 0.5-1 μM. This site is distinct from the oligosaccharide binding site and differs from previously identified sites of binding to thrombospondin and GABARAP (4-aminobutyrate type A receptor-associated protein). Although the arm domain of calreticulin was incapable of suppressing aggregation or binding hydrophobic peptides on its own, it did contribute to aggregation suppression in the context of the whole molecule. The high resolution x-ray crystal structure of calreticulin with a partially truncated arm domain reveals a marked difference in the relative orientations of the arm and lectin domains when compared with calnexin. Furthermore, a hydrophobic patch was detected on the arm domain that mediates crystal packing and may contribute to calreticulin chaperone function.
authors.affiliation: Department of Biochemistry, University of Toronto, Toronto, Ontario M5S 1A8, Canada.
authors.forename: Cosmin L
authors.initials: CL
authors.lastname: Pocanschi
authors.forename: Guennadi
authors.initials: G
authors.lastname: Kozlov
authors.forename: Ulf
authors.initials: U
authors.lastname: Brockmeier
authors.forename: Achim
authors.initials: A
authors.lastname: Brockmeier
authors.forename: David B
authors.initials: DB
authors.lastname: Williams
authors.forename: Kalle
authors.initials: K
authors.lastname: Gehring
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D037282:Calreticulin / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D002942:Circular Dichroism; D003460:Crystallization; D017931:DNA Primers; D018076:DNA, Complementary; D006801:Humans; D037102:Lectins / Q000737:chemistry* / Q000502:physiology*; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D013050:Spectrometry, Fluorescence
chemical_list: D037282:Calreticulin; D017931:DNA Primers; D018076:DNA, Complementary; D037102:Lectins