title: Role of tachykinin receptors in the modulation of colonic peristaltic activity in mice.
abstract: Tachykinins are important mediators of neuroneuronal and neuromuscular transmission in the gastrointestinal tract, however their contribution to colonic peristalsis in mice remains unclear. Therefore, our aim was to characterise the functional role of tachykinins in mediating peristalsis by evaluating the effect of selective tachykinin NK(1), NK(2) and NK(3) receptor agonists and antagonists on in vitro colonic peristaltic activity in mice. Using a modified Trendelenburg set-up, gradual distension of proximal and distal colonic segments evoked rhythmic, aborally migrating contractions. Peristaltic activity was assessed by quantifying the amplitude and interval of the corresponding pressure waves. Stimulation of NK(1) receptors showed regional differences as both the pressure amplitude and interval were enhanced in the distal colon without affecting peristalsis proximally. Blockade of NK(1) receptors reduced the peristaltic pressure amplitude in the proximal and distal colon while the interval was not significantly altered. NK(2) receptor stimulation resulted in a modest enhancement of the amplitude in proximal and distal segments and a slightly prolonged interval distally. Blockade of NK(2) receptors reduced the peristaltic pressure amplitude and interval in the distal colon. NK(3) receptor stimulation significantly augmented the amplitude in both segments and prolonged the interval distally. However, NK(3) receptor blockade had no effect on peristaltic activity. In conclusion, tachykinins contribute to colonic peristalsis in mice by acting mainly on NK(1) and NK(2) receptors and their effects show a proximal-to-distal gradient. NK(3) receptors might play a role in conditions of excess tachykinin release but appear not to be involved under the conditions of the present study.
authors.affiliation: Laboratory of Experimental Medicine and Pediatrics, Division of Gastroenterology, University of Antwerp, Universiteitsplein 1, B-2610 Antwerp, Belgium.
authors.forename: Annemie
authors.initials: A
authors.lastname: Deiteren
authors.forename: Benedicte Y
authors.initials: BY
authors.lastname: De Winter
authors.forename: Sara
authors.initials: S
authors.lastname: Nullens
authors.forename: Paul A
authors.initials: PA
authors.lastname: Pelckmans
authors.forename: Joris G
authors.initials: JG
authors.lastname: De Man
mesh_terms: D000818:Animals; D001285:Atropine / Q000494:pharmacology; D003106:Colon / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D018738:Hexamethonium / Q000494:pharmacology; D066298:In Vitro Techniques; D051379:Mice; D010528:Peristalsis / Q000187:drug effects; D021381:Protein Transport / Q000187:drug effects; D018039:Receptors, Tachykinin / Q000819:agonists / Q000037:antagonists & inhibitors / Q000378:metabolism*; D015320:Tachykinins / Q000378:metabolism; D013779:Tetrodotoxin / Q000494:pharmacology
chemical_list: D018039:Receptors, Tachykinin; D015320:Tachykinins; D018738:Hexamethonium; D013779:Tetrodotoxin; D001285:Atropine