title: Development and multiplexing of microsatellite markers in the polyploid perennial herb, Menyanthes trifoliata (Menyanthaceae).
abstract: OBJECTIVE
  We developed microsatellite primers to investigate genetic diversity and population genetic structure of the endangered herb Menyanthes trifoliata.
  
  
  RESULTS
  Using the microsatellite-enriched library method, we identified 10 primer pairs in M. trifoliata. The primers amplified nine di- and one tri-nucleotide repeats with 4-13 alleles per locus in two Belgian populations.
  
  
  CONCLUSIONS
  The results indicate that these markers offer an appropriate amount of variation to investigate genetic diversity, pollen dispersal (through paternity inference), and other conservation issues.
authors.affiliation: National Botanic Garden of Belgium, Domein van Bouchout, B-1860 Meise, Belgium.
authors.forename: Jana
authors.initials: J
authors.lastname: Raabová
authors.forename: Guillaume
authors.initials: G
authors.lastname: Hans
authors.forename: Ange-Marie
authors.initials: AM
authors.lastname: Risterucci
authors.forename: Anne-Laure
authors.initials: AL
authors.lastname: Jacquemart
authors.forename: Olivier
authors.initials: O
authors.lastname: Raspé