title: Purification and biochemical characterization of antioxidant peptide from horse mackerel (Magalaspis cordyla) viscera protein.
abstract: In the present study, a peptide having high antioxidant properties was isolated from horse mackerel viscera protein, Magalaspis cordyla. In vitro gastrointestinal digestion was employed to obtain potential protein hydrolysate and was subjected to consecutive chromatographic methods using fast protein liquid chromatography (FPLC) connected to diethyl amino ethyl (DEAE) anion exchange column and Sephadex G-25 gel filtration column. The activity of the fractions was tested against DPPH and hydroxyl radicals and the isolated peptide showed 89.2 and 59.1 percentage of scavenging. The amino acid sequence of purified peptide was determined using ESI-MS/MS as Ala-Cys-Phe-Leu (518.5 Da), it exhibited high activity against polyunsaturated fatty acid (PUFA) peroxidation than that of natural antioxidant, α-tocopherol.
authors.affiliation: Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur 603203, Tamil Nadu, India.
authors.forename: N S
authors.initials: NS
authors.lastname: Sampath Kumar
authors.forename: R A
authors.initials: RA
authors.lastname: Nazeer
authors.forename: R
authors.initials: R
authors.lastname: Jaiganesh
mesh_terms: D000818:Animals; D001713:Biphenyl Compounds / Q000037:antagonists & inhibitors* / Q000378:metabolism; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D017665:Hydroxyl Radical / Q000037:antagonists & inhibitors* / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D009842:Oligopeptides / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D010473:Perciformes / Q000378:metabolism*; D010851:Picrates / Q000037:antagonists & inhibitors* / Q000378:metabolism; D011492:Protein Hydrolysates / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D014781:Viscera / Q000737:chemistry* / Q000378:metabolism; D024502:alpha-Tocopherol / Q000494:pharmacology
chemical_list: D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D009842:Oligopeptides; D010851:Picrates; D011492:Protein Hydrolysates; D017665:Hydroxyl Radical; C004931:1,1-diphenyl-2-picrylhydrazyl; D024502:alpha-Tocopherol