title: Activity-dependent neuroprotective protein modulates its own gene expression.
abstract: We investigated whether activity-dependent neuroprotective protein (ADNP) could autoregulate its own expression. Both the endogenous ADNP gene and reporter gene constructs were analysed in response to overexpression of ADNP, supplied either as wild-type ADNP or a mutant form lacking the NAP motif, a motif which has neuroprotective properties. Overexpression of these two forms of ADNP resulted in both decreased endogenous ADNP expression and repressed ADNP promoter-directed reporter gene activity. Chromatin immunoprecipitation demonstrated the ability of ADNP to bind to its own promoter which is consistent with its action as a repressor of both promoter-supported and endogenous ADNP expression.
authors.affiliation: Institute of Translational Medicine, Sherrington Building, Ashton Street, University of Liverpool, Liverpool, L69 3GE, UK.
authors.forename: Moutasem S
authors.initials: MS
authors.lastname: Aboonq
authors.forename: Sylvia A
authors.initials: SA
authors.lastname: Vasiliou
authors.forename: Kate
authors.initials: K
authors.lastname: Haddley
authors.forename: John P
authors.initials: JP
authors.lastname: Quinn
authors.forename: Vivien J
authors.initials: VJ
authors.lastname: Bubb
mesh_terms: D045744:Cell Line, Tumor; D005786:Gene Expression Regulation / Q000502:physiology*; D017930:Genes, Reporter / Q000235:genetics; D006367:HeLa Cells; D018398:Homeodomain Proteins / Q000235:genetics*; D006801:Humans; D009419:Nerve Tissue Proteins / Q000235:genetics*; D009474:Neurons / Q000502:physiology*; D011401:Promoter Regions, Genetic / Q000235:genetics
chemical_list: C492178:ADNP protein, human; D018398:Homeodomain Proteins; D009419:Nerve Tissue Proteins