title: Correlation between stimulation strength and onset time of signal traveling within the neocortical neural circuits under caffeine application.
abstract: In general, strength of input to neocortical neural circuits affects the amplitude of postsynaptic potentials (PSPs), thereby modulating the way signals are transmitted within the circuits. Caffeine is one of the pharmacological agents able to modulate synaptic activities. The present study investigated how strength of input affects signal propagation in neocortical circuits under the application of caffeine. Spatio-temporal neural activities were observed from visual cortical slices of rats using optical recording methods with voltage-sensitive dye. Electrical stimulations were applied to white matter in the primary visual cortex with bath-application of caffeine. When the strength of stimulation was 0.3mA, signals propagated from the site of stimulation in the primary visual cortex toward the secondary visual cortex along vertical and horizontal pathways. When stimulation strength was reduced from 0.3mA to 0.07mA, start of signal propagation was delayed about 25ms without affecting field PSP amplitude or the manner of signal propagation. Conversely, co-application of caffeine and d-2-amino-5-phosphonovaleric acid (d-AP5) did not induce delays in signal start. These findings suggest that conversion of neural code from amplitude code to temporal code is inducible at the level of neocortical circuits in an N-methyl-d-aspartate (NMDA) receptor activity-dependent manner.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Kanazawa Medical University, Uchinada-cho, Ishikawa 920-0293, Japan. hyoshimu@kanazawa-med.ac.jp
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yoshimura
authors.forename: Miho
authors.initials: M
authors.lastname: Honjo
authors.forename: Tokio
authors.initials: T
authors.lastname: Sugai
authors.forename: Keiseki
authors.initials: K
authors.lastname: Kaneyama
authors.forename: Natsuki
authors.initials: N
authors.lastname: Segami
authors.forename: Nobuo
authors.initials: N
authors.lastname: Kato
mesh_terms: D000818:Animals; D002110:Caffeine / Q000494:pharmacology*; D004558:Electric Stimulation; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D019579:Neocortex / Q000187:drug effects / Q000502:physiology*; D009415:Nerve Net / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*; D013997:Time Factors; D014793:Visual Cortex / Q000187:drug effects / Q000502:physiology
chemical_list: D002110:Caffeine