title: Exceptional production of both prodigiosin and cycloprodigiosin as major metabolic constituents by a novel marine bacterium, Zooshikella rubidus S1-1.
abstract: A Gram-negative, red-pigment-producing marine bacterial strain, designated S1-1, was isolated from the tidal flat sediment of the Yellow Sea, Korea. On the basis of phenotypic, phylogenetic, and genetic data, strain S1-1 (KCTC 11448BP) represented a new species of the genus Zooshikella. Thus, we propose the name Zooshikella rubidus sp. nov. Liquid chromatography and mass spectrometry of the red pigments produced by strain S1-1 revealed that the major metabolic compounds were prodigiosin and cycloprodigiosin. In addition, this organism produced six minor prodigiosin analogues, including two new structures that were previously unknown. To our knowledge, this is the first description of a microorganism that simultaneously produces prodigiosin and cycloprodigiosin as two major metabolites. Both prodigiosin and cycloprodigiosin showed antimicrobial activity against several microbial species. These bacteria were approximately 1.5-fold more sensitive to cycloprodigiosin than to prodigiosin. The metabolites also showed anticancer activity against human melanoma cells, which showed significantly more sensitivity to prodigiosin than to cycloprodigiosin. The secondary metabolite profiles of strain S1-1 and two reference bacterial strains were compared by liquid chromatography-mass spectrometry. Multivariate statistical analyses based on secondary metabolite profiles by liquid chromatography-mass spectrometry indicated that the metabolite profile of strain S1-1 could clearly be distinguished from those of two phylogenetically related, prodigiosin-producing bacterial strains.
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-333, Republic of Korea.
authors.forename: Jong Suk
authors.initials: JS
authors.lastname: Lee
authors.forename: Yong-Sook
authors.initials: YS
authors.lastname: Kim
authors.forename: Sooyeon
authors.initials: S
authors.lastname: Park
authors.forename: Jihoon
authors.initials: J
authors.lastname: Kim
authors.forename: So-Jung
authors.initials: SJ
authors.lastname: Kang
authors.forename: Mi-Hwa
authors.initials: MH
authors.lastname: Lee
authors.forename: Sangryeol
authors.initials: S
authors.lastname: Ryu
authors.forename: Jong Myoung
authors.initials: JM
authors.lastname: Choi
authors.forename: Tae-Kwang
authors.initials: TK
authors.lastname: Oh
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Yoon
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000378:metabolism / Q000494:pharmacology; D045744:Cell Line, Tumor / Q000187:drug effects; D002853:Chromatography, Liquid; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D020563:Gammaproteobacteria / Q000235:genetics / Q000378:metabolism*; D005838:Genotype; D006801:Humans; D007211:Indoles / Q000378:metabolism*; D013058:Mass Spectrometry; D008545:Melanoma; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D010860:Pigments, Biological / Q000096:biosynthesis* / Q000235:genetics; D011353:Prodigiosin / Q000031:analogs & derivatives* / Q000096:biosynthesis*; D011758:Pyrroles / Q000378:metabolism*; D012329:RNA, Bacterial / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012623:Seawater / Q000382:microbiology
chemical_list: D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D007211:Indoles; D010860:Pigments, Biological; D011758:Pyrroles; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; C108328:cycloprodigiosin; D011353:Prodigiosin