title: Phytoestrogen calycosin-7-O-β-D-glucopyranoside ameliorates advanced glycation end products-induced HUVEC damage.
abstract: Vasculopathy including endothelial cell (EC) apoptosis and inflammation contributes to the high incidence of stroke and myocardial infarction in diabetic patients. The aim of the present study was to investigate the effect of calycosin-7-O-β-D-glucopyranoside (CG), a phytoestrogen, on advanced glycation end products (AGEs)-induced HUVEC damage. We observed that CG can significantly ameliorate AGEs-induced HUVEC oxidative stress and apoptosis. The ratio of SOD/MDA was significantly increased to the normal level by CG pretreatment. CG preincubation dramatically increased anti-apoptotic Bcl-2 while decreased pro-apoptotic Bax and Bad expressions as detected by immunocytochemistry. Moreover, CG ameliorated macrophage migration and adhesion to HUVEC; the monocyte chemotactic protein-1 and interleukin-6 levels in the culture supernatant were dramatically reduced by CG as determined by ELISA; the expressions of inflammatory proteins including ICAM-1, TGF-β1, and RAGE in both protein and mRNA levels were significantly reduced to the normal level by CG pretreatment as determined by immunocytochemistry and real-time RT-PCR. The intracellular investigation suggests that CG can reverse AGEs-activated ERK1/2 and NF-κB phosphorylation, in which estrogen receptors were involved in. Our results strongly indicate that CG can modulate EC dysfunction by ameliorating AGEs-induced cell apoptosis and inflammation.
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Faculty of Chinese Medicine, Macau University of Science and Technology, Avenida Wai Long, Taipa, Macau.
authors.forename: Youhua
authors.initials: Y
authors.lastname: Xu
authors.forename: Liang
authors.initials: L
authors.lastname: Feng
authors.forename: Shanshan
authors.initials: S
authors.lastname: Wang
authors.forename: Quan
authors.initials: Q
authors.lastname: Zhu
authors.forename: Jing
authors.initials: J
authors.lastname: Lin
authors.forename: Chihan
authors.initials: C
authors.lastname: Lou
authors.forename: Ping
authors.initials: P
authors.lastname: Xiang
authors.forename: Bao
authors.initials: B
authors.lastname: He
authors.forename: Zhaoguang
authors.initials: Z
authors.lastname: Zheng
authors.forename: Dan
authors.initials: D
authors.lastname: Tang
authors.forename: Guoying
authors.initials: G
authors.lastname: Zuo
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects*; D004797:Enzyme-Linked Immunosorbent Assay; D005960:Glucosides / Q000494:pharmacology*; D017127:Glycation End Products, Advanced / Q000494:pharmacology*; D006801:Humans; D007150:Immunohistochemistry; D007529:Isoflavones / Q000494:pharmacology*; D008264:Macrophages / Q000166:cytology / Q000187:drug effects; D051379:Mice; D048789:Phytoestrogens / Q000494:pharmacology*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D005960:Glucosides; D017127:Glycation End Products, Advanced; D007529:Isoflavones; D048789:Phytoestrogens; C488311:calycosin-7-O-glucopyranoside