title: Successful extraction of DNA from archived alcohol-fixed white-eye fish specimens using an ancient DNA protocol.
abstract: A protocol used routinely for rapid ancient DNA extraction was applied to fish tissue archived over 80 years ago. The method proved successful, whereas other extraction protocols failed. Researchers working on DNA from older archived fish samples are encouraged to continue to concentrate their efforts on 'white-eye' specimens, which indicate an alcohol-based fixative and are thus likely to yield viable DNA.
authors.affiliation: Molecular Ecology and Fisheries Genetics Laboratory, Environment Centre for Wales, Biological Sciences, Bangor University, Deiniol Road, Bangor LL57 2UW, UK. markus.debruyn@gmail.com
authors.forename: M
authors.initials: M
authors.lastname: de Bruyn
authors.forename: L R
authors.initials: LR
authors.lastname: Parenti
authors.forename: G R
authors.initials: GR
authors.lastname: Carvalho
mesh_terms: D000818:Animals; D023761:Beloniformes / Q000235:genetics*; D003247:Conservation of Natural Resources / Q000379:methods; D003532:Cyprinodontiformes / Q000235:genetics*; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification*; D005404:Fixatives / Q000737:chemistry; D005821:Genetic Techniques
chemical_list: D005404:Fixatives; D004247:DNA