title: Direct chromatographic separation of racemates on the basis of isotopic chirality.
abstract: Direct separation was achieved between two isomers which are enantiomeric to each other by virtue of the presence of hydrogen/deuterium isotopes. A racemic mixture of the R- and S-isomers of phenyl(phenyl-d(5))methanol was separated by high-performance liquid chromatography using cellulose tribenzoate-coated silica as a stationary phase and a 2-propanol/hexane (5/95) mixture as a mobile phase, and the absolute configuration of each separated isomer was identified. The cellulose derivative showed preferential retention of (R)-(-)-phenyl(phenyl-d(5))methanol compared to the (S)-(+)-isomer, with a separation factor of 1.0080 based on the preferential binding of a C(6)D(5) group over a C(6)H(5) group to the primary binding site.
authors.affiliation: Nacalai Tesque, 17 Ishibashi, Kaide-cho, Muko-shi, Kyoto 617, Japan, and Department of Polymer Science and Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kimata
authors.forename: K
authors.initials: K
authors.lastname: Hosoya
authors.forename: T
authors.initials: T
authors.lastname: Araki
authors.forename: N
authors.initials: N
authors.lastname: Tanaka