title: Mitochondrial DNA variation in natural populations of endangered Indian feather-back fish, Chitala chitala.
abstract: Genetic variation at mitochondrial cytochrome b (cyt b) and D-loop region reveals the evidence of population sub-structuring in Indian populations of highly endangered primitive feather-back fish Chitala chitala. Samples collected through commercial catches from eight riverine populations from different geographical locations of India were analyzed for cyt b region (307 bp) and D-loop region (636-716 bp). The sequences of the both the mitochondrial regions revealed high haplotype diversity and low nucleotide diversity. The patterns of genetic diversity, haplotypes networks clearly indicated two distinct mitochondrial lineages and mismatch distribution strongly suggest a historical influence on the genetic structure of C. chitala populations. The baseline information on genetic variation and the evidence of population sub-structuring generated from this study would be useful for planning effective strategies for conservation and rehabilitation of this highly endangered species.
authors.affiliation: National Bureau of Fish Genetic Resources, Canal Ring Road, PO Dilkusha, Lucknow, 226002 UP, India.
authors.forename: Anup
authors.initials: A
authors.lastname: Mandal
authors.forename: Vindhya
authors.initials: V
authors.lastname: Mohindra
authors.forename: Rajeev Kumar
authors.initials: RK
authors.lastname: Singh
authors.forename: Peyush
authors.initials: P
authors.lastname: Punia
authors.forename: Ajay Kumar
authors.initials: AK
authors.lastname: Singh
authors.forename: Kuldeep Kumar
authors.initials: KK
authors.lastname: Lal
mesh_terms: D000818:Animals; D001483:Base Sequence; D016000:Cluster Analysis; D003247:Conservation of Natural Resources / Q000379:methods; D045303:Cytochromes b / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics*; D056727:Endangered Species; D005399:Fishes / Q000235:genetics*; D014644:Genetic Variation; D005828:Genetics, Population; D005843:Geography; D006239:Haplotypes / Q000235:genetics; D007194:India; D008969:Molecular Sequence Data; D058974:Phylogeography; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D004272:DNA, Mitochondrial; D045303:Cytochromes b