title: Activity control of the ClpC adaptor McsB in Bacillus subtilis.
abstract: Controlled protein degradation is an important cellular reaction for the fast and efficient adaptation of bacteria to ever-changing environmental conditions. In the low-GC, Gram-positive model organism Bacillus subtilis, the AAA+ protein ClpC requires specific adaptor proteins not only for substrate recognition but also for chaperone activity. The McsB adaptor is activated particularly during heat stress, allowing the controlled degradation of the CtsR repressor by the ClpCP protease. Here we report how the McsB adaptor becomes activated by autophosphorylation on specific arginine residues during heat stress. In nonstressed cells McsB activity is inhibited by ClpC as well as YwlE.
authors.affiliation: Institute of Microbiology, Ernst Moritz Arndt University Greifswald, Greifswald, Germany.
authors.forename: A K W
authors.initials: AK
authors.lastname: Elsholz
authors.forename: K
authors.initials: K
authors.lastname: Hempel
authors.forename: S
authors.initials: S
authors.lastname: Michalik
authors.forename: K
authors.initials: K
authors.lastname: Gronau
authors.forename: D
authors.initials: D
authors.lastname: Becher
authors.forename: M
authors.initials: M
authors.lastname: Hecker
authors.forename: U
authors.initials: U
authors.lastname: Gerth
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010766:Phosphorylation; D011494:Protein Kinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; C087481:ClpC protein, Bacteria; D006360:Heat-Shock Proteins; D011494:Protein Kinases; C423322:McsB protein, Bacillus subtilis