title: Immunohistochemical observation of canine degenerative myelopathy in two Pembroke Welsh Corgi dogs.
abstract: Immunohistochemistry was performed to assess whether oxidative stress and/or denatured proteins play roles in the pathogenesis of canine degenerative myelopathy (DM). Two Pembroke Welsh Corgi (PWC) dogs with a homozygous mutation (c.118G>A) in the canine superoxide dismutase 1 (SOD1) gene were examined. The pathological features of the dogs were consistent with those of previous cases of DM in PWC. In the spinal lesions, diffuse SOD1 expression was observed in the neurons while no inclusion-like aggregates had formed, which disagreed with the findings of a previous study. A unique inducible nitric oxide synthase (iNOS) staining pattern in reactive astrocytes and a significant increase in ubiquitin immunoreactivity in the spinal lesions were also observed. These findings indicate the involvement of oxidative stress and the accumulation of ubiquitinated proteins in the pathogenesis of canine DM, whereas the role of SOD1 remains unclear.
authors.affiliation: Department of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo 1-1-1 Yayoi, Bunkyoku, Tokyo 113-8657, Japan.
authors.forename: Mizue
authors.initials: M
authors.lastname: Ogawa
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Uchida
authors.forename: Eun-Sil
authors.initials: ES
authors.lastname: Park
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kamishina
authors.forename: Jun
authors.initials: J
authors.lastname: Sasaki
authors.forename: Hye-Sook
authors.initials: HS
authors.lastname: Chang
authors.forename: Osamu
authors.initials: O
authors.lastname: Yamato
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nakayama
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000378:metabolism*; D004285:Dogs; D007150:Immunohistochemistry; D008297:Male; D019636:Neurodegenerative Diseases / Q000378:metabolism / Q000662:veterinary*; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D018384:Oxidative Stress; D011489:Protein Denaturation; D013118:Spinal Cord Diseases / Q000378:metabolism / Q000662:veterinary*; D013482:Superoxide Dismutase / Q000378:metabolism*; D000072105:Superoxide Dismutase-1; D025801:Ubiquitin / Q000378:metabolism
chemical_list: D025801:Ubiquitin; D052247:Nitric Oxide Synthase Type II; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1