title: Methanol extract from mycelium of endophytic fungus Rhizoctonia sp. induces antinociceptive and anti-inflammatory activities in mice.
abstract: The present study aimed to elucidate the antinociceptive and anti-inflammatory properties of the methanol extract from the mycelium of the endophytic fungus Rhizoctonia sp. (MEMRh) in mice. The antinociceptive activity was assessed using the abdominal constriction, hot plate, and formalin tests. The anti-inflammatory activity was assessed using a murine model of paw edema. Intraperitoneal administration of MEMRh (0.1, 1, 10 and 100 mg/kg, i.p.) produced an inhibition of acetic acid-induced writhing in mice for at least 8 h. In addition, all doses tested of the methanol extract were able to prevent thermal nociception in the hot-plate test. Furthermore, treatment with MEMRh (10 mg/kg, i.p.) inhibited both the early and late phases of formalin-induced nociception. This antinociceptive effect exhibited by MEMRh in the formalin test was reversed by the systemic administration of naloxone. MEMRh produced inhibition in a carrageenan-induced edema model at a dose of 10 mg/kg. The same extract also displayed significant activity against a histamine- or PGE(2)-induced edema model. The experimental data demonstrated that MEMRh showed remarkable anti-inflammatory and antinociceptive activities. Further studies are warranted to define and isolate the active anti-inflammatory and antinociceptive components from this endophytic fungus, which may yield effective agents for the treatment of inflammatory disorders.
authors.affiliation: Laboratório de Biologia Celular, Núcleo de Pesquisa Multidisciplinar, Instituto de Ciências Biológicas e da Saúde, Maceió, Alagoas, Brazil.
authors.forename: Betijane Soares
authors.initials: BS
authors.lastname: de Barros
authors.forename: Juliane Pereira
authors.initials: JP
authors.lastname: da Silva
authors.forename: Jamylle Nunes
authors.initials: JN
authors.lastname: de Souza Ferro
authors.forename: Isabela Karine Rodrigues
authors.initials: IK
authors.lastname: Agra
authors.forename: Fabíola
authors.initials: F
authors.lastname: de Almeida Brito
authors.forename: Erica Dutra
authors.initials: ED
authors.lastname: Albuquerque
authors.forename: Luiz Carlos
authors.initials: LC
authors.lastname: Caetano
authors.forename: Emiliano
authors.initials: E
authors.lastname: Barreto
mesh_terms: D000700:Analgesics / Q000737:chemistry / Q000627:therapeutic use*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000627:therapeutic use*; D002351:Carrageenan / Q000633:toxicity; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy*; D007213:Indomethacin / Q000627:therapeutic use; D008297:Male; D000432:Methanol / Q000737:chemistry*; D051379:Mice; D010147:Pain Measurement; D012232:Rhizoctonia / Q000737:chemistry*
chemical_list: D000700:Analgesics; D000893:Anti-Inflammatory Agents; D002351:Carrageenan; D007213:Indomethacin; D000432:Methanol