title: DARIO: a ncRNA detection and analysis tool for next-generation sequencing experiments.
abstract: Small non-coding RNAs (ncRNAs) such as microRNAs, snoRNAs and tRNAs are a diverse collection of molecules with several important biological functions. Current methods for high-throughput sequencing for the first time offer the opportunity to investigate the entire ncRNAome in an essentially unbiased way. However, there is a substantial need for methods that allow a convenient analysis of these overwhelmingly large data sets. Here, we present DARIO, a free web service that allows to study short read data from small RNA-seq experiments. It provides a wide range of analysis features, including quality control, read normalization, ncRNA quantification and prediction of putative ncRNA candidates. The DARIO web site can be accessed at http://dario.bioinf.uni-leipzig.de/.
authors.affiliation: Interdisciplinary Center for Bioinformatics and Bioinformatics Group, Department of Computer Science, University Leipzig, Germany.
authors.forename: Mario
authors.initials: M
authors.lastname: Fasold
authors.forename: David
authors.initials: D
authors.lastname: Langenberger
authors.forename: Hans
authors.initials: H
authors.lastname: Binder
authors.forename: Peter F
authors.initials: PF
authors.lastname: Stadler
authors.forename: Steve
authors.initials: S
authors.lastname: Hoffmann
mesh_terms: D059014:High-Throughput Nucleotide Sequencing; D020407:Internet; D022661:RNA, Untranslated / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D017423:Sequence Analysis, RNA; D012984:Software; D014584:User-Computer Interface
chemical_list: D022661:RNA, Untranslated