title: [Clinical study of dynamic change of inflammatory factors in serum of acute paraquat rats].
abstract: OBJECTIVE
  To investigate the change of inflammatory factor in lung tissue of acute paraquat (PQ) poisoned rats.
  
  
  METHODS
  hundred SD rats were randomly divided into two groups: the normal control group (NC group, n = 10) and the PQ group (n = 80). The 1 ml saline was administered once in normal control group. The PQ group was administered with 25 mg/kg 1% PQ by intraperitoneal injection to establish the model of PQ induced renal injury. At six hours, at the first, the third and the seventh day the PQ group were sacrificed, while at the first day the normal control group was sacrificed. The level of normal tumor necrosis factor-α (TNF-α), interleukin-2 (IL-2), IL-6 in serum of rats were detected. Meanwhile, pathological changes of the renal were examined under optical microscope.
  
  
  RESULTS
  Histopathological findings of an earlier, a large number of patients edema clearly inflammatory cell infiltration. Compared with the control group, PQ exposure of serum TNF-α, IL-2, IL-6, the level at each time point were elevated. PQ treated group 6 h and 1, 3, 7 d when the IL-2 levels were (2.16 ± 0.65), (2.95 ± 1.02), (3.05 ± 1.12), (2.21 ± 0.62) µg/L, IL-6 were (62.5 ± 8.6), (85.6 ± 13.5), (90.3 ± 15.6), (65.3 ± 9.1) ng/ml, TNF-α were (1.95 ± 0.53), (2.86 ± 0.92), (3.15 ± 1.02), (2.06 ± 0.71) µg/L, compared with the control group, are significantly higher, the differences were statistically significant (P < 0.01).
  
  
  CONCLUSIONS
  acute PQ poisoning serum TNF-α, IL-2, IL-6 levels were significantly increased both early and late inflammatory factors involved in PQ poisoning the pathogenesis of renal injury.
authors.affiliation: Laboratory Medicine of the First Affiliated Hospital of Xinxiang Medical College, Weihui Henan Province, 453100, China.
authors.forename: Lu-yang
authors.initials: LY
authors.lastname: Jiao
authors.forename: Zhi-shan
authors.initials: ZS
authors.lastname: Song
authors.forename: Qing-he
authors.initials: QH
authors.lastname: Guo
authors.forename: Guang-jian
authors.initials: GJ
authors.lastname: Lu
mesh_terms: D000818:Animals; D007376:Interleukin-2 / Q000097:blood*; D015850:Interleukin-6 / Q000097:blood*; D007668:Kidney / Q000473:pathology; D008297:Male; D010269:Paraquat / Q000506:poisoning*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000097:blood*
chemical_list: D007376:Interleukin-2; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D010269:Paraquat