title: [Role of mPGES-1 in the occurrence, progression, metastasis and invasion of hepatocellular carcinoma].
abstract: OBJECTIVE
  To study the expression of mPGES-1 in hepatocellular carcinoma (HCC), observe the effect of MK886 on down-regulation of mPGES-1 gene expression on the biology of human hepatocarcinoma cell line HepG2 and to investigate its significance in the occurrence, progression, metastasis and invasion.
  
  
  METHODS
  HCC tissues, para-carcinoma tissues, far-carcinoma tissues and normal liver tissues were collected. The expressions of mPGES-1 were determined by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot. The proliferation, adherence, migration and invasion abilities of HepG2 cells interfered by MK886 were assessed by MTT and transwell technique respectively.
  
  
  RESULTS
  The expression of mPGES-1 in HCCs was higher than that in normal liver tissues (P < 0.01), which increased following histological grade. Furthermore, mPGES-1 expression level was higher in the capsule invasion and metastasis tumor than in primary locus. A significant dose-dependent down-regulation of expressions of mPGES-1 gene mRNA and protein were observed in HepG2 cells when MK886 was given for 48 h (F = 140.402, P < 0.01; a'= 0.00714, P < 0.01). Compared with the control group, the growth inhibitory rate of HepG2 cell was observed significantly time and dose-dependent when MK886 was given. The rate of adhesion cells in experimental groups were 85.3% ± 1.3%, 70.5% ± 1.5% and 45.8% ± 2.4%, respectively, less than that in control group 100.0% ± 0 (F = 626.313, P < 0.01). The migration cells was 92.47 ± 1.90, 62.63 ± 1.96 and 37.33 ± 0.83 respectively in the experimental groups after 24 h, lower than that in the control group 128.93 ± 2.60 (F = 1253.805, P < 0.01). The invasion assay revealed that the invading cells were 41.67 ± 1.30, 25.47 ± 1.30 and 13.93 ± 1.66 in the experimental groups, in contrast to 55.67 ± 2.08 in control group after 24 h. The difference between these groups was significant (F = 372.615, P < 0.01). The numbers of adhesion, migration and invasion of HepG2 cells were dose-dependent in MK886 groups.
  
  
  CONCLUSIONS
  Over-expression of mPGES-1 was associated with the tumorigenesis and progression of HCC. The down-regulation of mPGES-1 gene expression might indicated the decrease of the invasion and metastasis of HCC.
authors.affiliation: Department of Pathology, Institute of Oncology, Fujian Medical University, Fuzhou 350004, China.
authors.forename: Yuan-e
authors.initials: YE
authors.lastname: Lian
authors.forename: Jing-feng
authors.initials: JF
authors.lastname: Liu
authors.forename: Xiao-jun
authors.initials: XJ
authors.lastname: Wang
authors.forename: Sheng-bing
authors.initials: SB
authors.lastname: Zang
authors.forename: Ai-min
authors.initials: AM
authors.lastname: Huang
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000473:pathology*; D002448:Cell Adhesion; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D056945:Hep G2 Cells; D006801:Humans; D007211:Indoles / Q000494:pharmacology; D019746:Intramolecular Oxidoreductases / Q000378:metabolism*; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008861:Microsomes / Q000378:metabolism; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D000071876:Prostaglandin-E Synthases; D002722:Chlorobenzenes
chemical_list: D007211:Indoles; D019746:Intramolecular Oxidoreductases; D000071876:Prostaglandin-E Synthases; C060893:MK-886; D002722:Chlorobenzenes; C000605862:PTGES protein, human