title: High-throughput single-molecule fluorescence spectroscopy using parallel detection.
abstract: Solution-based single-molecule fluorescence spectroscopy is a powerful new experimental approach with applications in all fields of natural sciences. The basic concept of this technique is to excite and collect light from a very small volume (typically femtoliter) and work in a concentration regime resulting in rare burst-like events corresponding to the transit of a single-molecule. Those events are accumulated over time to achieve proper statistical accuracy. Therefore the advantage of extreme sensitivity is somewhat counterbalanced by a very long acquisition time. One way to speed up data acquisition is parallelization. Here we will discuss a general approach to address this issue, using a multispot excitation and detection geometry that can accommodate different types of novel highly-parallel detector arrays. We will illustrate the potential of this approach with fluorescence correlation spectroscopy (FCS) and single-molecule fluorescence measurements obtained with different novel multipixel single-photon counting detectors.
authors.affiliation: Dept of Chemistry & Biochemistry, Los Angeles, CA, USA 90095.
authors.forename: X
authors.initials: X
authors.lastname: Michalet
authors.forename: R A
authors.initials: RA
authors.lastname: Colyer
authors.forename: G
authors.initials: G
authors.lastname: Scalia
authors.forename: T
authors.initials: T
authors.lastname: Kim
authors.forename: Moran
authors.initials: M
authors.lastname: Levi
authors.forename: Daniel
authors.initials: D
authors.lastname: Aharoni
authors.forename: Adrian
authors.initials: A
authors.lastname: Cheng
authors.forename: F
authors.initials: F
authors.lastname: Guerrieri
authors.forename: Katsushi
authors.initials: K
authors.lastname: Arisaka
authors.forename: Jacques
authors.initials: J
authors.lastname: Millaud
authors.forename: I
authors.initials: I
authors.lastname: Rech
authors.forename: D
authors.initials: D
authors.lastname: Resnati
authors.forename: S
authors.initials: S
authors.lastname: Marangoni
authors.forename: A
authors.initials: A
authors.lastname: Gulinatti
authors.forename: M
authors.initials: M
authors.lastname: Ghioni
authors.forename: S
authors.initials: S
authors.lastname: Tisa
authors.forename: F
authors.initials: F
authors.lastname: Zappa
authors.forename: S
authors.initials: S
authors.lastname: Cova
authors.forename: S
authors.initials: S
authors.lastname: Weiss