title: Adipose tissue-deprived stem cells acquire cementoblast features treated with dental follicle cell conditioned medium containing dentin non-collagenous proteins in vitro.
abstract: Adipose tissue-derived stem cells (ADSCs), which are easily harvested and show excellent pluripotency potential, have generated considerable interest in regenerative medicine. In this study, the differentiation of ADSCs was assessed after treatment with dental follicle cell conditioned medium (DFCCM) containing dentin non-collagenous proteins (dNCPs). ADSCs exhibited a fibroblast-like morphology and high proliferative capacity. However, after treatment with dNCPs/DFCCM, ADSCs changed from a fibroblast-like to cementoblast-like morphology and significantly lost their proliferative capacity. Alkaline phosphatase activity and in vitro mineralization behaviour of ADSCs were significantly enhanced. Mineralization-related markers including cementum attachment protein, bone sialoprotein, osteocalcin, osteopontin and osteonectin were detected at mRNA or protein levels, whereas dentin sialophosphoprotein and dentin sialoprotein were not detected, implying a cementoblast-like phenotype. These results demonstrate that ADSCs acquired cementoblast features in vitro with dNCPs/DFCCM treatment and could be a potential source of cementogenic cells for periodontal regeneration.
authors.affiliation: Department of Stomatology, Daping Hospital & Research Institute of Surgery, Third Military Medical University, 10 Daping Changjiang Branch Road, Yuzhong District, Chongqing 400042, China.
authors.forename: Xiujie
authors.initials: X
authors.lastname: Wen
authors.forename: Xin
authors.initials: X
authors.lastname: Nie
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Luchuan
authors.initials: L
authors.lastname: Liu
authors.forename: Manjing
authors.initials: M
authors.lastname: Deng
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D001861:Bone Regeneration; D002454:Cell Differentiation / Q000187:drug effects*; D002469:Cell Separation / Q000379:methods; D017077:Culture Media, Conditioned / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D003739:Dental Cementum / Q000166:cytology*; D003795:Dental Sac / Q000378:metabolism*; D003804:Dentin / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D010519:Periodontium / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects*; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D017077:Culture Media, Conditioned; D016326:Extracellular Matrix Proteins