title: Process optimization by decoupled control of key microbial populations: distribution of activity and abundance of polyphosphate-accumulating organisms and nitrifying populations in a full-scale IFAS-EBPR plant.
abstract: This study investigated the abundance and distribution of key functional microbial populations and their activities in a full-scale integrated fixed film activated sludge-enhanced biological phosphorus removal (IFAS-EBPR) process. Polyphosphate accumulating organisms (PAOs) including Accumulibacter and EBPR activities were predominately associated with the mixed liquor (>90%) whereas nitrifying populations and nitrification activity resided mostly (>70%) on the carrier media. Ammonia oxidizer bacteria (AOB) were members of the Nitrosomonas europaea/eutropha/halophila and the Nitrosomonas oligotropha lineages, while nitrite oxidizer bacteria (NOB) belonged to the Nitrospira genus. Addition of the carrier media in the hybrid activated sludge system increased the nitrification capacity and stability; this effect was much greater in the first IFAS stage than in the second one where the residual ammonia concentration becomes limiting. Our results show that IFAS-EBPR systems enable decoupling of solid residence time (SRT) control for nitrifiers and PAOs that require or prefer conflicting SRT values (e.g. >15 days required for nitrifiers and <5 days preferred for PAOs). Allowing the slow-growing nitrifiers to attach to the carrier media and the faster-growing phosphorus (P)-removing organisms (and other heterotrophs, e.g. denitrifiers) to be in the suspended mixed liquor (ML), the EBPR-IFAS system facilitates separate SRT controls and overall optimization for both N and P removal processes.
authors.affiliation: Civil and Environmental Engineering Department, Northeastern University, 400 Snell Engineering Center, 360 Huntington Ave, Boston, MA 02115, USA. aonnis@coe.neu.edu
authors.forename: Annalisa
authors.initials: A
authors.lastname: Onnis-Hayden
authors.forename: Nehreen
authors.initials: N
authors.lastname: Majed
authors.forename: Andreas
authors.initials: A
authors.lastname: Schramm
authors.forename: April Z
authors.initials: AZ
authors.lastname: Gu
mesh_terms: D000641:Ammonia / Q000378:metabolism; D001673:Biodegradation, Environmental; D018441:Biofilms; D009573:Nitrites / Q000378:metabolism*; D043370:Nitrosomonas europaea / Q000378:metabolism; D010758:Phosphorus / Q000378:metabolism; D011122:Polyphosphates / Q000378:metabolism*; D012722:Sewage / Q000382:microbiology*
chemical_list: D009573:Nitrites; D011122:Polyphosphates; D012722:Sewage; D010758:Phosphorus; D000641:Ammonia