title: Quantitative Carré differential interference contrast microscopy to assess phase and amplitude.
abstract: We present a method of using an unmodified differential interference contrast microscope to acquire quantitative information on scatter and absorption of thin tissue samples. A simple calibration process is discussed that uses a standard optical wedge. Subsequently, we present a phase-stepping procedure for acquiring phase gradient information exclusive of absorption effects. The procedure results in two-dimensional maps of the local angular (polar and azimuthal) ray deviation. We demonstrate the calibration process, discuss details of the phase-stepping algorithm, and present representative results for a porcine skin sample.
authors.affiliation: Portland State University, 1900 SW 4th Avenue, Portland, Oregon 97201, USA. donald.duncan@pdx.edu
authors.forename: Donald D
authors.initials: DD
authors.lastname: Duncan
authors.forename: David G
authors.initials: DG
authors.lastname: Fischer
authors.forename: Amanda
authors.initials: A
authors.lastname: Dayton
authors.forename: Scott A
authors.initials: SA
authors.lastname: Prahl
mesh_terms: D000818:Animals; D008853:Microscopy / Q000379:methods*; D055620:Optical Phenomena; D012867:Skin / Q000166:cytology; D013552:Swine