title: Identification of tripartite motif-containing 22 (TRIM22) as a novel NF-κB activator.
abstract: Increasing evidence suggests that TRIM family proteins may play important roles in the regulation of innate immune signaling pathways. Here we report TRIM22 is involved in the activation of NF-κB. It was found that overexpression of TRIM22 could dose-dependently activate NF-κB as demonstrated by reporter gene assay and electrophoretic mobility shift assay, but had no effect on the activity of other transcription factors, including NF-AT, AP-1, C/EBP and IRFs. Further study showed that both the N-terminal RING domain and C-terminal SPRY domain were crucial for TRIM22-mediated NF-κB activation. Moreover, our results revealed that TRIM22 overexpression could significantly induce the secretion of pro-inflammatory cytokines by human macrophage cell line U937 in an NF-κB-dependent manner. These data suggested that TRIM22 was a positive regulator of NF-κB-mediated transcription.
authors.affiliation: Institute for Immunobiology, Department of Immunology, Shanghai Medical College of Fudan University, Shanghai 200032, PR China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Yu
authors.forename: Bo
authors.initials: B
authors.lastname: Gao
authors.forename: Zhijian
authors.initials: Z
authors.lastname: Duan
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.forename: Sidong
authors.initials: S
authors.lastname: Xiong
mesh_terms: D024202:Electrophoretic Mobility Shift Assay; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D057809:HEK293 Cells; D006801:Humans; D015778:Minor Histocompatibility Antigens; D016328:NF-kappa B / Q000819:agonists* / Q000378:metabolism; D054829:RING Finger Domains / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D000070584:Tripartite Motif Proteins; D020298:U937 Cells
chemical_list: D015778:Minor Histocompatibility Antigens; D016328:NF-kappa B; D012097:Repressor Proteins; C094314:TRIM22 protein, human; D000070584:Tripartite Motif Proteins