title: Age-related binding of proximal region of ApoE promoter to nuclear proteins of mouse cerebral cortex.
abstract: Mouse ApolipoproteinE (ApoE) gene has maximum promoter activity in the proximal region (-212 to +54) which includes different regulatory elements. These elements bind to specific protein factors and influence the expression of genes which are involved in key brain functions that decline with age. As there is no information on the binding of apoE promoter to nuclear proteins as a function of age, we have analyzed the binding of USF, AP1 and one negative element sequence present in ApoE proximal promoter to nuclear proteins of the cerebral cortex of mice of different ages. The findings show the formation of one complex with USF and two complexes with AP1 and negative element. The intensity of these complexes varies with age, indicating differential binding of protein factors to specific elements of apoE promoter, which reflect age-related regulation of apoE -mediated brain functions.
authors.affiliation: Biochemistry and Molecular Biology Laboratory, Department of Zoology, Banaras Hindu University, Varanasi 221 005, India.
authors.forename: Sarika
authors.initials: S
authors.lastname: Singh
authors.forename: M K
authors.initials: MK
authors.lastname: Thakur
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D001057:Apolipoproteins E / Q000235:genetics* / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism*; D008297:Male; D051379:Mice; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D018808:Transcription Factor AP-1 / Q000235:genetics / Q000378:metabolism; D051720:Upstream Stimulatory Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D001057:Apolipoproteins E; D009687:Nuclear Proteins; D018808:Transcription Factor AP-1; D051720:Upstream Stimulatory Factors