title: Development of an enhanced chemiluminescence ELISA for the rapid detection of acrylamide in food products.
abstract: In this work, a polyclonal antibody for acrylamide (AA) was obtained by immunization of rabbits with N-acryloxysuccinimide (NAS) and keyhole limpet hemocyanin (KLH) conjugate. A direct enzyme-linked immunosorbent assay (ELISA) based on this antibody was developed with enhanced chemiluminescent (ECL) detection of AA in food samples. Assay conditions, such as concentrations of antibody and enzyme conjugate and competition time, were optimized. The effects of ionic strength and pH value were investigated. The optimized ECL-ELISA system allowed AA determination in a linear working range of 26.3-221.1 ng mL(-1) with an IC(50) value of 60.6 ng mL(-1) and a limit of detection of 18.6 ng mL(-1). Good recoveries with spiked food samples were obtained with a recovery range from 74.4 to 98.1%, and these results correlated well with those obtained using an HPLC method. This indicates that ECL-ELISA is applicable to the specific detection and routine monitoring of AA in food samples.
authors.affiliation: Changshu Institute of Technology, Changshu 215500, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Quan
authors.forename: Menling
authors.initials: M
authors.lastname: Chen
authors.forename: Yuehua
authors.initials: Y
authors.lastname: Zhan
authors.forename: Genhua
authors.initials: G
authors.lastname: Zhang
mesh_terms: D020106:Acrylamide / Q000032:analysis* / Q000276:immunology; D000818:Animals; D000906:Antibodies / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D008163:Luminescent Measurements / Q000379:methods*; D011817:Rabbits
chemical_list: D000906:Antibodies; D020106:Acrylamide