title: Temperature and salt concentration alter base-sequence selectivity of a duplex DNA-binding protein.
abstract: A structural polymorphism of nucleic acids, which depends on the concentration of cations and the conditions of hydration, are strongly involved with interactions between DNA and proteins. In this paper, we report that different DNA sequences bound to hyperthermostable TATA-box-binding protein (PhoTBP) at different combinations of temperature and salt concentration in in vitro selection experiments. As a result of the interaction of-these selected DNAs with PhoTBP, characteristic changes in the numbers of water molecules and ions occurred under each condition of the selection experiment. This finding could help us to understand the solvent environment-dependent preference for base sequences in protein–DNA interactions.
authors.affiliation: Department of Medical Genome Sciences, Graduate School of Frontier Sciences, University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8562, Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Nagatoishi
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Motonori
authors.initials: M
authors.lastname: Kudou
authors.forename: Kouhei
authors.initials: K
authors.lastname: Tsumoto
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D007477:Ions; D008969:Molecular Sequence Data; D011485:Protein Binding; D043902:Pyrococcus horikoshii / Q000737:chemistry; D012965:Sodium Chloride / Q000737:chemistry*; D035181:TATA-Box Binding Protein / Q000737:chemistry*; D013696:Temperature; D014867:Water / Q000737:chemistry
chemical_list: D007477:Ions; D035181:TATA-Box Binding Protein; D014867:Water; D012965:Sodium Chloride; D004247:DNA