title: The regulation of CD4+ T cell immune responses toward Th2 cell development by prostaglandin E2.
abstract: As an important immune mediator, PGE2 plays an important role in the immune tolerance, autoimmune diseases, immune regulation and tumor immunotolerance. PGE2 is considered to be a promising candidate for the control of the immune diseases. To further understand the immuno-modulating effects of PGE2 on CD4+ T cells, in vitro investigation was conducted in the present study. The results showed that PGE2 inhibited the proliferation of T cells in vitro in a dose-dependent manner. Gene expression profiling showed that 1716 genes were down regulated and 73 genes were up regulated with a change of 1.5 fold. Several signal transduction pathways were involved, such as TNF-α and NF-kB signaling pathway, T cell receptor signaling pathway, IL-2 signaling pathway, and MAPK pathway. The results showed that PGE2 inhibited IFN-γ, TNF-α and IL-4 production by CD4+ T cells 24h after cell culture. A comparison between IFN-γ and IL-4 production showed that PGE2 enhanced the relative ratio of IL-4 to IFN-γ in CD4+ T cells culture, and regulated CD4+ T cells toward Th2 cell development. The results of the present study indicated that PGE2 has the potential to treat Th1-mediated inflammatory diseases by regulating CD4+ T cells toward Th2 cell immune response.
authors.affiliation: Department of Nephrology, The First Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Yu-Shi
authors.initials: YS
authors.lastname: Bao
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
authors.forename: Ru-Juan
authors.initials: RJ
authors.lastname: Xie
authors.forename: Mei
authors.initials: M
authors.lastname: Wang
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Wang
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zhou
authors.forename: Wen-Jing
authors.initials: WJ
authors.lastname: Zhai
authors.forename: Si-Zhou
authors.initials: SZ
authors.lastname: Feng
authors.forename: Ming-Zhe
authors.initials: MZ
authors.lastname: Han
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D015232:Dinoprostone / Q000494:pharmacology*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects / Q000276:immunology; D006801:Humans; D007111:Immunity, Cellular / Q000187:drug effects; D056747:Immunomodulation; D016328:NF-kappa B / Q000378:metabolism; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects / Q000276:immunology; D058408:Th1-Th2 Balance / Q000187:drug effects; D018418:Th2 Cells / Q000276:immunology / Q000378:metabolism* / Q000473:pathology
chemical_list: D016207:Cytokines; D016328:NF-kappa B; D016693:Receptors, Antigen, T-Cell, alpha-beta; D048049:Extracellular Signal-Regulated MAP Kinases; D015232:Dinoprostone