title: Microbial volatile-induced accumulation of exceptionally high levels of starch in Arabidopsis leaves is a process involving NTRC and starch synthase classes III and IV.
abstract: Microbial volatiles promote the accumulation of exceptionally high levels of starch in leaves. Time-course analyses of starch accumulation in Arabidopsis leaves exposed to fungal volatiles (FV) emitted by Alternaria alternata revealed that a microbial volatile-induced starch accumulation process (MIVOISAP) is due to stimulation of starch biosynthesis during illumination. The increase of starch content in illuminated leaves of FV-treated hy1/cry1, hy1/cry2, and hy1/cry1/cry2 Arabidopsis mutants was many-fold lower than that of wild-type (WT) leaves, indicating that MIVOISAP is subjected to photoreceptor-mediated control. This phenomenon was inhibited by cordycepin and accompanied by drastic changes in the Arabidopsis transcriptome. MIVOISAP was also accompanied by enhancement of the total 3-phosphoglycerate/Pi ratio, and a two- to threefold increase of the levels of the reduced form of ADP-glucose pyrophosphorylase. Using different Arabidopsis knockout mutants, we investigated the impact in MIVOISAP of downregulation of genes directly or indirectly related to starch metabolism. These analyses revealed that the magnitude of the FV-induced starch accumulation was low in mutants impaired in starch synthase (SS) classes III and IV and plastidial NADP-thioredoxin reductase C (NTRC). Thus, the overall data showed that Arabidopsis MIVOISAP involves a photocontrolled, transcriptionally and post-translationally regulated network wherein photoreceptor-, SSIII-, SSIV-, and NTRC-mediated changes in redox status of plastidial enzymes play important roles.
authors.affiliation: Instituto de Agrobiotecnología, Nafarroa, Spain.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Ignacio
authors.initials: I
authors.lastname: Ezquer
authors.forename: Abdellatif
authors.initials: A
authors.lastname: Bahaji
authors.forename: Manuel
authors.initials: M
authors.lastname: Montero
authors.forename: Miroslav
authors.initials: M
authors.lastname: Ovecka
authors.forename: Edurne
authors.initials: E
authors.lastname: Baroja-Fernández
authors.forename: Francisco José
authors.initials: FJ
authors.lastname: Muñoz
authors.forename: Angel
authors.initials: A
authors.lastname: Mérida
authors.forename: Goizeder
authors.initials: G
authors.lastname: Almagro
authors.forename: Maite
authors.initials: M
authors.lastname: Hidalgo
authors.forename: María Teresa
authors.initials: MT
authors.lastname: Sesma
authors.forename: Javier
authors.initials: J
authors.lastname: Pozueta-Romero
mesh_terms: D000528:Alternaria / Q000166:cytology / Q000472:pathogenicity; D000596:Amino Acids / Q000096:biosynthesis; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology*; D029681:Arabidopsis Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D055786:Gene Knockout Techniques; D017343:Genes, Plant; D051599:Glucose-1-Phosphate Adenylyltransferase / Q000378:metabolism; D005964:Glucosyltransferases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D054884:Host-Pathogen Interactions / Q000235:genetics / Q000502:physiology; D008954:Models, Biological; D009154:Mutation; D055519:Photoreceptors, Plant / Q000378:metabolism; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology; D013213:Starch / Q000378:metabolism*; D013214:Starch Synthase / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D013880:Thioredoxin-Disulfide Reductase / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D014199:Trehalose / Q000378:metabolism; D055549:Volatile Organic Compounds / Q000633:toxicity; D001614:beta-Amylase / Q000378:metabolism
chemical_list: D000596:Amino Acids; D029681:Arabidopsis Proteins; D055519:Photoreceptors, Plant; D055549:Volatile Organic Compounds; D013213:Starch; D014199:Trehalose; D013880:Thioredoxin-Disulfide Reductase; D005964:Glucosyltransferases; C504530:starch synthase III, Arabidopsis; D013214:Starch Synthase; C520418:starch synthase IV, Arabidopsis; C496715:ADP-glucose pyrophosphorylase, Arabidopsis; D051599:Glucose-1-Phosphate Adenylyltransferase; D001614:beta-Amylase