title: Real-time snapshot hyperspectral imaging endoscope.
abstract: Hyperspectral imaging has tremendous potential to detect important molecular biomarkers of early cancer based on their unique spectral signatures. Several drawbacks have limited its use for in vivo screening applications: most notably the poor temporal and spatial resolution, high expense, and low optical throughput of existing hyperspectral imagers. We present the development of a new real-time hyperspectral endoscope (called the image mapping spectroscopy endoscope) based on an image mapping technique capable of addressing these challenges. The parallel high throughput nature of this technique enables the device to operate at frame rates of 5.2 frames per second while collecting a (x, y, λ) datacube of 350 × 350 × 48. We have successfully imaged tissue in vivo, resolving a vasculature pattern of the lower lip while simultaneously detecting oxy-hemoglobin.
authors.affiliation: Department of Bioengineering, Rice University, 6100 Main Street, Houston, Texas 77005, USA.
authors.forename: Robert T
authors.initials: RT
authors.lastname: Kester
authors.forename: Noah
authors.initials: N
authors.lastname: Bedard
authors.forename: Liang
authors.initials: L
authors.lastname: Gao
authors.forename: Tomasz S
authors.initials: TS
authors.lastname: Tkaczyk
mesh_terms: D003199:Computer Systems; D019723:Endoscopes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005336:Fiber Optic Technology / Q000295:instrumentation*; D006801:Humans; D008046:Lip / Q000166:cytology; D012031:Refractometry / Q000295:instrumentation*; D013057:Spectrum Analysis / Q000295:instrumentation