title: Zinc finger protein, multitype 1, suppresses human Th2 development via downregulation of IL-4.
abstract: BACKGROUND
  Among several GATA family transcription factor-associating proteins, zinc finger protein, multitype 1 (ZFPM1), at least that of murine origin, has been shown to modulate the activity of GATA-3. However, the functional role of human ZFPM1 in the immune system has not been elucidated. Therefore, we here investigated the contribution of ZFPM1 to human Th1/Th2 differentiation.
  
  
  METHODS
  The cDNA of ZFPM1 was cloned and introduced into human cord blood CD4+ T cells by a lentiviral transduction system. Then, the expression of IL-4 and IFN-γ mRNA was determined by quantitative real-time RT-PCR. The effect of ZFPM1 on the promoter activity of IL-4 and IFN-γ in Jurkat cells was also investigated.
  
  
  RESULTS
  Stimulation-induced expression of IL-4 and IFN-γ in human CD4+ T cells was suppressed and enhanced, respectively, by the introduction of ZFPM1. The transcriptional activity of IL-4 was also diminished by ZFPM1, whereas that of IFN-γ was not affected.
  
  
  CONCLUSIONS
  ZFPM1 that facilitates human Th1 differentiation via the downregulation of IL-4 is a potential target for the treatment of allergic diseases.
authors.affiliation: Clinical Research Center for Allergy and Rheumatology, Sagamihara, Japan.
authors.forename: Noriko
authors.initials: N
authors.lastname: Kitamura
authors.forename: Akio
authors.initials: A
authors.lastname: Mori
authors.forename: Hideki
authors.initials: H
authors.lastname: Tatsumi
authors.forename: Soichi
authors.initials: S
authors.lastname: Nemoto
authors.forename: Takachika
authors.initials: T
authors.lastname: Hiroi
authors.forename: Osamu
authors.initials: O
authors.lastname: Kaminuma
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology* / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D002454:Cell Differentiation / Q000276:immunology*; D015536:Down-Regulation / Q000235:genetics*; D005312:Fetal Blood / Q000166:cytology; D015870:Gene Expression / Q000187:drug effects / Q000276:immunology; D005786:Gene Expression Regulation / Q000276:immunology*; D017930:Genes, Reporter / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism; D015847:Interleukin-4 / Q000235:genetics / Q000378:metabolism*; D015759:Ionomycin / Q000494:pharmacology; D019169:Jurkat Cells; D016086:Lentivirus / Q000235:genetics; D008164:Luminescent Proteins / Q000235:genetics; D008213:Lymphocyte Activation / Q000276:immunology; D009687:Nuclear Proteins / Q000502:physiology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D018417:Th1 Cells / Q000166:cytology; D018418:Th2 Cells / Q000166:cytology*; D014157:Transcription Factors / Q000502:physiology*; D014161:Transduction, Genetic
chemical_list: D001426:Bacterial Proteins; C508598:IL4 protein, human; D008164:Luminescent Proteins; D009687:Nuclear Proteins; D014157:Transcription Factors; C498432:ZFPM1 protein, human; C054562:yellow fluorescent protein, Bacteria; D015847:Interleukin-4; D015759:Ionomycin; D007371:Interferon-gamma; D013755:Tetradecanoylphorbol Acetate