title: [Hepatocyte growth factor combined with autologous bone marrow mesenchymal stem cell transplantation for treatment of silicosis].
abstract: OBJECTIVE
  To evaluate the potential role of hepatocyte growth factor (HGF) combined with bone marrow mesenchymal stem cells (BMSC) autograft for the treatment of silicosis.
  
  
  METHODS
  Bone marrow (100 ml) was aspirated from a severe silicosis patient. BMSCs isolated, purified and cultured in vitro. When BMSC came to 70% confluence at passage 3, the culture medium was added liposomes (lipo2000) and plasmid-HGF (p-HGF) and cultured for 2 d. HGF-MSCSs (5 × 10(7) cells) were resuspended in 50 ml 0.9% sodium chloride (NS) and infused Intravenous drip at 3 consecutive times (once a week). Clinical follow-up were performed before and after treatment: (1) pulmonary high-kV X-ray, chest CT examination; (2) pulmonary function test; (3) determination of serum ceruloplasmin.
  
  
  RESULTS
  The symptoms such as coughing, chest tightness disappeared at 12 months after treatment. Pulmonary function tests showed significant changes after treatment: forced vital capacity (FVC) increased from 64.6% to 81.0%, forced expiratory volume in one second (FEV(1.0)) increased from 68.7% to 90.1%, 1 second rate (FEV(1.0)/FVC%) reduced from 111.6% to 107.1%, the maximum mid-expiratory flow (FEF(25%∼75%) decreased from 100.2% to 94.6%, forced expiratory vital capacity 75% of the moment bit of gas flow (MEF(75%)) increased from 99.2% to 113.5%, forced expiratory vital capacity 50% of the moment bit of gas flow (MEF(50%)) increased from 125.3% to 130.2%, forced expiratory vital capacity 25% of the moment bit of gas flow (MEF(25%)) reduced from 86.9% to 71.7%; serum ceruloplasmin levels decreased from 690 mg/L to 180.6 mg/L; lung high-kV X-ray at 1st review showed that diffuse lung nodules had been absorbed and getting smaller than before treatment; chest CT showed that the distribution and number of small nodules at double lung fields decreased than before treatment.
  
  
  CONCLUSIONS
  HGF combined with BMSC transplantation may have some potential role for the treatment of silicosis patients.
authors.affiliation: Department of Internal Medicine, Guangzhou Municipal Twelfth People's Hospital, Guangzhou 510620, China.
authors.forename: Wei-wei
authors.initials: WW
authors.lastname: Liu
authors.forename: Jia-yu
authors.initials: JY
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Yu
authors.forename: Geng-xin
authors.initials: GX
authors.lastname: Ye
authors.forename: Cheng
authors.initials: C
authors.lastname: Zhang
authors.forename: Zhi-qian
authors.initials: ZQ
authors.lastname: Yang
authors.forename: Yi-ming
authors.initials: YM
authors.lastname: Liu
authors.forename: Xue-yun
authors.initials: XY
authors.lastname: Zhong
authors.forename: Zi-kuan
authors.initials: ZK
authors.lastname: Guo
mesh_terms: D000328:Adult; D016026:Bone Marrow Transplantation; D005260:Female; D005500:Follow-Up Studies; D017228:Hepatocyte Growth Factor / Q000627:therapeutic use*; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation; D012829:Silicosis / Q000628:therapy*; D016896:Treatment Outcome
chemical_list: D017228:Hepatocyte Growth Factor