title: The bovine herpesvirus type 1 envelope protein Us9 acidic domain is crucial for anterograde axonal transport.
abstract: In this study, we examined the functional role of bovine herpesvirus type 1 (BHV-1) Us9 acidic domain residues 83-90 in the anterograde axonal transport of the virus in calves (natural host), rabbits, and in cultured neurons. A mutant virus strain lacking Us9 residues 83-90 (BHV-1 Us9 Δ83-90) and the rescued virus (BHV-1 Us9 R83-90) replicated efficiently in the nasal and ocular epithelium during primary infection and established latency in the trigeminal ganglia (TG). However, upon reactivation from latency, only the BHV-1 Us9 R83-90 virus was detected in nasal and ocular swabs of animals. In compartmentalized, rabbit primary dorsal root ganglia (DRG) neuron cultures, the Us9-deleted BHV-1, BHV-1 Us9 Δ83-90 and BHV-1 Us9 R83-90 viruses were transported efficiently in the retrograde direction. However, only the BHV-1 Us9 R83-90 virus was transported in an anterograde direction. These studies suggested that the Us9 acidic domain residues located between 83 and 90 were required for axonal anterograde transport.
authors.affiliation: Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA. Chowdh@lsu.edu
authors.forename: S I
authors.initials: SI
authors.lastname: Chowdhury
authors.forename: M C S
authors.initials: MC
authors.lastname: Brum
authors.forename: C
authors.initials: C
authors.lastname: Coats
authors.forename: A
authors.initials: A
authors.lastname: Doster
authors.forename: Huiyong
authors.initials: H
authors.lastname: Wei
authors.forename: C
authors.initials: C
authors.lastname: Jones
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001370:Axonal Transport; D002417:Cattle; D002460:Cell Line; D005727:Ganglia, Spinal / Q000821:virology; D006566:Herpesviridae Infections / Q000662:veterinary* / Q000821:virology; D007242:Herpesvirus 1, Bovine / Q000502:physiology*; D008969:Molecular Sequence Data; D009474:Neurons / Q000821:virology; D017434:Protein Structure, Tertiary; D011817:Rabbits; D012668:Trigeminal Ganglion / Q000821:virology; D014759:Viral Envelope Proteins / Q000737:chemistry* / Q000378:metabolism*
chemical_list: C524555:US9 protein, bovine herpesvirus 1; D014759:Viral Envelope Proteins