title: Spectroscopic signature of mouse embryonic stem cell-derived hepatocytes using synchrotron Fourier transform infrared microspectroscopy.
abstract: Stem cell-based therapy for liver regeneration has been proposed to overcome the persistent shortage in the supply of suitable donor organs. A requirement for this to succeed is to find a rapid method to detect functional hepatocytes, differentiated from embryonic stem cells. We propose Fourier transform infrared (FTIR) microspectroscopy as a versatile method to identify the early and last stages of the differentiation process leading to the formation of hepatocytes. Using synchrotron-FTIR microspectroscopy, the means of identifying hepatocytes at the single-cell level is possible and explored. Principal component analysis and subsequent partial least-squares (PLS) discriminant analysis is applied to distinguish endoderm induction from hepatic progenitor cells and matured hepatocyte-like cells. The data are well modeled by PLS with endoderm induction, hepatic progenitor cells, and mature hepatocyte-like cells able to be discriminated with very high sensitivity and specificity. This method provides a practical tool to monitor endoderm induction and has the potential to be applied for quality control of cell differentiation leading to hepatocyte formation.
authors.affiliation: Synchrotron Light Research Institute, Muang, Nakhon Ratchasima 30000, Thailand.
authors.forename: Kanjana
authors.initials: K
authors.lastname: Thumanu
authors.forename: Waraporn
authors.initials: W
authors.lastname: Tanthanuch
authors.forename: Danna
authors.initials: D
authors.lastname: Ye
authors.forename: Anawat
authors.initials: A
authors.lastname: Sangmalee
authors.forename: Chanchao
authors.initials: C
authors.lastname: Lorthongpanich
authors.forename: Rangsun
authors.initials: R
authors.lastname: Parnpai
authors.forename: Philip
authors.initials: P
authors.lastname: Heraud
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000502:physiology*; D022781:Hepatocytes / Q000166:cytology* / Q000502:physiology*; D051379:Mice; D017550:Spectroscopy, Fourier Transform Infrared / Q000295:instrumentation* / Q000379:methods; D017356:Synchrotrons / Q000295:instrumentation*