title: [Cloning and sequencing analysis of a novel allele HLA-A*02:251].
abstract: OBJECTIVE
  To identify a novel human leukocyte antigen (HLA) allele A*02:251 and analyze the sequences in Chinese population.
  
  
  METHODS
  Routine HLA-A, -B, -DRB1 high resolution genotyping for healthy Chinese donors and patients was performed with polymerase chain reaction-sequence based typing. An unknown HLA-A allele was initially detected by HLA typing in the healthy donor. Genomic DNA of the HLA-A locus in the proband was amplified, the amplified product was cloned by PMD18-T to split the two alleles, and selected clones were sequenced.
  
  
  RESULTS
  The sequencing results showed that a normal A*02:06:01 and a novel A*02:251 variant allele were identified. The sequence of the novel allele has been submitted to GenBank (HM245348). Nucleotide sequence alignments with HLA-A allele from the IMGT/HLA Sequence Database showed that the novel A*02 variant allele differed from the closest allele A*02:01:01:01 by nt 383 G to C (codon 128 GAG to GAC) in exon 3, which resulted in one amino acid substitution of Glu to Asp. The HLA-A, B, C and DQB1 alleles of the healthy donor did not match with that of the patient.
  
  
  CONCLUSIONS
  This novel allele is officially designated as HLA-A*02:251 by World Health Organization(WHO) Nomenclature Committee (Submission ID HWS10010755). The sequence of HLA-A locus in exon 3 is confirmed to be polymorphic in Chinese population.
authors.affiliation: Shenzhen Blood Center Institute, Shenzhen HLA Laboratory of China Marrow Donor Program, Shenzhen, Guangdong, 518035 P. R. China.
authors.forename: Shi-zheng
authors.initials: SZ
authors.lastname: Jin
authors.forename: Su-qing
authors.initials: SQ
authors.lastname: Gao
authors.forename: Hong-yan
authors.initials: HY
authors.lastname: Zou
authors.forename: Zhi-hui
authors.initials: ZH
authors.lastname: Deng
mesh_terms: D000483:Alleles; D001483:Base Sequence; D003001:Cloning, Molecular; D005091:Exons / Q000235:genetics; D005838:Genotype; D015234:HLA-A Antigens / Q000235:genetics*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D015234:HLA-A Antigens