title: Curcumin and resveratrol synergistically stimulate p21 and regulate cox-2 by maintaining adequate zinc levels during lung carcinogenesis.
abstract: This study explored the efficacy of curcumin and resveratrol in maintaining adequate zinc levels to regulate p21 and cyclooxygenase-2 (cox-2) during benzo[a]pyrene (BP)-induced lung carcinogenesis. The mice were segregated into five groups, which included normal control, BP treated, BP plus curcumin treated, BP plus resveratrol treated, and BP plus curcumin plus resveratrol-treated groups. BP treatment resulted in a significant decrease in the zinc levels and protein expression of p21. On the contrary, the enzyme activity of cox-2 showed a significant increase in the BP-treated mice. Interestingly, combined supplementation of curcumin and resveratrol to BP-treated mice resulted in an appreciable improvement in the zinc levels and protein expression of p21. In contrast, synergistic supplementation with phytochemicals resulted in a significant decrease in the enzyme activities of cox-2 in BP-treated mice. This study, therefore, concludes that combined treatment with curcumin and resveratrol maintains adequate zinc levels and regulates inflammation by cox-2 and cell cycle arrest by p21 during lung carcinogenesis in mice.
authors.affiliation: Department of Biophysics, Panjab University, Chandigarh, India.
authors.forename: Anshoo
authors.initials: A
authors.lastname: Malhotra
authors.forename: Praveen
authors.initials: P
authors.lastname: Nair
authors.forename: Devinder Kumar
authors.initials: DK
authors.lastname: Dhawan
mesh_terms: D000818:Animals; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use*; D001564:Benzo(a)pyrene / Q000633:toxicity; D015153:Blotting, Western; D001835:Body Weight / Q000187:drug effects; D002273:Carcinogens / Q000633:toxicity; D003474:Curcumin / Q000008:administration & dosage; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000378:metabolism*; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D018450:Disease Progression; D004357:Drug Synergism; D007124:Immunoenzyme Techniques; D008175:Lung Neoplasms / Q000139:chemically induced / Q000378:metabolism* / Q000517:prevention & control*; D008297:Male; D051379:Mice; D009929:Organ Size / Q000187:drug effects; D000077185:Resveratrol; D013267:Stilbenes / Q000008:administration & dosage; D015032:Zinc / Q000378:metabolism*
chemical_list: D002273:Carcinogens; C493957:Cdkn1a protein, mouse; D050759:Cyclin-Dependent Kinase Inhibitor p21; D013267:Stilbenes; D001564:Benzo(a)pyrene; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2; D003474:Curcumin; D015032:Zinc; D000077185:Resveratrol