title: Association between genes encoding components of the IL-10/IL-0 receptor pathway and asthma in children.
abstract: BACKGROUND
  Asthma is a chronic inflammation of the airways associated with recurrent symptoms that range from mild to debilitating. Interleukin-10 (IL-10) is a cytokine that displays pleiotropic effects in asthma and allergy.
  
  
  OBJECTIVE
  To determine whether polymorphisms of IL-10/IL-10R pathway contribute to asthma susceptibility in Egyptian children.
  
  
  METHODS
  The IL-10 (-1082G/A), IL-10R1 (G330R), and signal transducer and activator of transcription 3 (STAT3) rs2293452 single-nucleotide polymorphism (SNP) were genotyped in 110 atopic children with asthma, 110 non-atopic children with asthma, and 110 healthy children. Serum IL-10 and immunoglobulin E (IgE) levels were determined by enzyme-linked immunosorbent assay.
  
  
  RESULTS
  A significant association was observed between the IL-10 polymorphism and asthma in both atopic (P = .03) and non-atopic asthma groups (P = .04). The genotype frequencies of IL-10R1 polymorphisms did not differ between all groups. We identified a significant association between STAT3 polymorphism and asthma susceptibility in atopic asthma (P < .001), whereas no such association was observed in the non-atopic asthma group (P = .9). No evidence of gene interactions was found.
  
  
  CONCLUSIONS
  Polymorphisms of IL-10 and STAT3 may be useful as a new DNA-based diagnostic biomarker for identifying high-risk children susceptible to asthma.
authors.affiliation: Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Egypt. yousrihussein@hotmail.com
authors.forename: Yousri M
authors.initials: YM
authors.lastname: Hussein
authors.forename: Sally M
authors.initials: SM
authors.lastname: Shalaby
authors.forename: Randa H
authors.initials: RH
authors.lastname: Mohamed
authors.forename: Tamer H
authors.initials: TH
authors.lastname: Hassan
mesh_terms: D001249:Asthma / Q000235:genetics* / Q000276:immunology*; D001483:Base Sequence; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay; D005838:Genotype; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood / Q000235:genetics; D007249:Inflammation; D016753:Interleukin-10 / Q000097:blood / Q000235:genetics*; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D053701:Receptors, Interleukin-10 / Q000235:genetics*; D012307:Risk Factors; D050796:STAT3 Transcription Factor / Q000235:genetics*; D017422:Sequence Analysis, DNA; D015398:Signal Transduction
chemical_list: D053701:Receptors, Interleukin-10; D050796:STAT3 Transcription Factor; D016753:Interleukin-10; D007073:Immunoglobulin E