title: Isolation and genetic analysis of bovine viral diarrhea virus from infected cattle in indiana.
abstract: Species and biotype distribution was determined in 44 bovine viral diarrhea virus- (BVDV-) positive samples submitted to the Animal Disease Diagnostic Laboratory (ADDL) in Indiana during 2006-2008. BVDV RNA was detected in the 5'-untranslated region and N(pro) region using reverse transcriptase PCR followed by sequencing analysis of the PCR product. Additionally, cases were classified into one of six categories according to history and/or lesions: acute symptomatic, hemorrhagic, respiratory distress, reproductive, persistent infection (PI), and mucosal disease (MD). Of 44 BVDV-positive samples, 33 were noncytopathic (ncp), 10 were cytopathic (cp), and one presented both ncp and cp biotypes. Sequencing analysis demonstrated that all samples belonged to BVDV-1a, BVDV-1b, or BVDV-2. The most common isolate was ncp BVDV-1b, (44%) followed by ncp BVDV-2a (24%). Among the six categories, respiratory clinical signs were the most common (36%) followed by PI (25%) and MD (16%).
authors.affiliation: Indiana Animal Disease Diagnostic Laboratory and Department of Comparative Pathobiology, Purdue University, West Lafayette, IN 47907, USA.
authors.forename: Roman M
authors.initials: RM
authors.lastname: Pogranichniy
authors.forename: Megan E
authors.initials: ME
authors.lastname: Schnur
authors.forename: Eran A
authors.initials: EA
authors.lastname: Raizman
authors.forename: Duane A
authors.initials: DA
authors.lastname: Murphy
authors.forename: Maria
authors.initials: M
authors.lastname: Negron
authors.forename: H Leon
authors.initials: HL
authors.lastname: Thacker