title: Simultaneous analysis of acetaminophen, p-aminophenol and aspirin metabolites by hydrophilic interaction and strong anion exchange capillary liquid chromatography coupled to amperometric detection.
abstract: A simple and sensitive method has been developed for the simultaneous determination of polar nonsteroidal pharmaceuticals and metabolites, including acetaminophen, p-aminophenol and several aspirin metabolites (salicylic acid, gentisic acid, salicyluric acid and 2,3-dihydroxybenzoic acid), by capillary liquid chromatography with amperometric detection. Using a capillary monolithic column with mixed mode stationary phases and a mobile phase composed of acetonitrile and Tris buffer, rapid separation of six polar analytes was achieved within 8 min, and a hydrophilic interaction and strong anion exchange separation mechanism were exhibited. Method detection limits of six analytes ranged from 10 to 50 ng/mL. In terms of precision, the intra- and interday relative standard deviation values in all analytes never exceeded 3.1% for migration time and 8.9% for peak areas, respectively. This method provided a simple, rapid and cost-effective approach for the analysis of polar pharmaceuticals. The applicability of the method in pharmacokinetics was verified by spiking human serum samples with the compounds and analyzing the recoveries.
authors.affiliation: Key Laboratory of Analysis and Detection Technology for Food Safety, Ministry of Education, Fuzhou University, Fuzhou, P. R. China; College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou, P. R. China.
authors.forename: Minmin
authors.initials: M
authors.lastname: Zheng
authors.forename: Yimin
authors.initials: Y
authors.lastname: Wu
authors.forename: Lanxiang
authors.initials: L
authors.lastname: Lu
authors.forename: Kang
authors.initials: K
authors.lastname: Ding
authors.forename: Fengxiang
authors.initials: F
authors.lastname: Tang
authors.forename: Zian
authors.initials: Z
authors.lastname: Lin
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Wu
mesh_terms: D000082:Acetaminophen / Q000097:blood* / Q000378:metabolism; D000368:Aged; D000369:Aged, 80 and over; D000627:Aminophenols / Q000097:blood* / Q000378:metabolism; D001241:Aspirin / Q000097:blood* / Q000378:metabolism; D002852:Chromatography, Ion Exchange / Q000295:instrumentation / Q000379:methods*; D005260:Female; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008297:Male
chemical_list: D000627:Aminophenols; D000082:Acetaminophen; D001241:Aspirin; C026729:4-aminophenol