title: Expression of secreted and membrane-bound mucins in the airways of piglets experimentally infected with Mycoplasma hyopneumoniae.
abstract: Immunohistochemistry was used to demonstrate secreted mucins MUC2, MUC5AC and MUC5B and membrane-bound mucin MUC4 in the pulmonary bronchioles of piglets experimentally infected with Mycoplasma hyopneumoniae. Conventional status, Landrace-Duroc cross-bred piglets, 13 days of age, were randomised to two groups. One group (n=20) was infected by the intra-tracheal route with the SNU98703 strain of M. hyopneumoniae, and a group of 12 animals acted as uninfected controls. Five infected and three uninfected piglets were euthanased on the day of infection and at 7, 21, and 35 days post-inoculation (PI). Membrane-bound MUC4 and secreted MUC5AC were the predominant mucins produced in the bronchioles of the piglets in response to M. hyopneumoniae infection, but by day 35 PI, all labelled mucins had returned to pre-infection levels, contemporaneous with reduced pulmonary lesion scores. The increased mucin production may result from direct stimulation of the epithelium by mycoplasmal infection, or may arise indirectly following M. hyopneumoniae-induced ciliostasis.
authors.affiliation: Seoul National University, College of Veterinary Medicine, Department of Veterinary Pathology, 599 Gwanak-ro, Gwanak-Gu, 151-742 Seoul, Republic of Korea.
authors.forename: C H
authors.initials: CH
authors.lastname: Kim
authors.forename: Y
authors.initials: Y
authors.lastname: Oh
authors.forename: K
authors.initials: K
authors.lastname: Han
authors.forename: H W
authors.initials: HW
authors.lastname: Seo
authors.forename: D
authors.initials: D
authors.lastname: Kim
authors.forename: C
authors.initials: C
authors.lastname: Park
authors.forename: I
authors.initials: I
authors.lastname: Kang
authors.forename: C
authors.initials: C
authors.lastname: Chae
mesh_terms: D000818:Animals; D000833:Animals, Suckling / Q000276:immunology* / Q000382:microbiology; D055745:Bronchioles / Q000276:immunology; D007150:Immunohistochemistry / Q000662:veterinary; D008562:Membrane Glycoproteins / Q000096:biosynthesis*; D009077:Mucins / Q000096:biosynthesis*; D045705:Mycoplasma hyopneumoniae / Q000276:immunology*; D011897:Random Allocation; D013552:Swine / Q000276:immunology* / Q000382:microbiology
chemical_list: D008562:Membrane Glycoproteins; D009077:Mucins