title: Crystallization and preliminary X-ray studies of a galactose-specific lectin from the seeds of Spatholobus parviflorus.
abstract: A galactose-specific seed lectin was purified from the legume Spatholobus parviflorus and crystallized using the hanging-drop vapour-diffusion technique. The crystals belonged to space group P1, with unit-cell parameters a = 60.998, b = 60.792, c = 78.179 Å, α = 101.32, β = 91.38, γ = 104.32°. X-ray diffraction data were collected under cryoconditions (100 K) to a resolution of 2.04 Å using a MAR image-plate detector system mounted on a rotating-anode X-ray (Cu Kα) generator. Molecular replacement using legume-lectin coordinates as a search model gave a tetrameric structure.
authors.affiliation: Department of Biotechnology and Microbiology, Kannur University, Kerala, India.
authors.forename: K
authors.initials: K
authors.lastname: Geethanandan
authors.forename: Joseph
authors.initials: J
authors.lastname: Abhilash
authors.forename: S R
authors.initials: SR
authors.lastname: Bharath
authors.forename: C
authors.initials: C
authors.lastname: Sadasivan
authors.forename: M
authors.initials: M
authors.lastname: Haridas
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D007887:Fabaceae / Q000737:chemistry*; D005690:Galactose / Q000737:chemistry; D037121:Plant Lectins / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry
chemical_list: D037121:Plant Lectins; D005690:Galactose