title: Development of recombinant rabies viruses vectors with Gaussia luciferase reporter based on Chinese vaccine strain CTN181.
abstract: The recombinant rabies virus (RV) vectors encoding the secreted gene marker Gaussia luciferase (Gluc) were generated based on Chinese vaccine strain CTN181. Vectors included replication competent CTN-Gluc, CTN/G(Q333R)-Gluc, in which the amino acid in position 333 of glycoprotein was mutated from glutamine (Q) to arginine (R), and replication constrained CTNΔG-Gluc, in which the glycoprotein encoding gene (G) was deleted. The growth of recombinant RVs in transfected cells was confirmed through biochemical assays of Gluc activities. Gluc expression in recombinant CTNΔG-Gluc virus was highest while that in CTN/G(Q333R)-Gluc virus was lowest. The optimal time to harvest recombinant RVs was determined and the function of pathogenic and nonpathogenic rabies glycoprotein in virus recovery was examined. The addition of glycoprotein was slightly beneficial for virus recovery and the titer of rescued virus was lowered even when the amino acid in G333 position of glycoprotein was mutated from nonpathogenic Gln to pathogenic Arg.
  
  
  CONCLUSIONS
  Viral vectors based on a human rabies vaccine strain CTN181 were successful. Gluc was useful as an in vitro gene marker for monitoring the growth of recombinant RVs iteratively in cell culture.
authors.affiliation: State Key Laboratory for Infectious Diseases Prevention and Control, State Key Laboratory for Molecular Virology and Genetic Engineering, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 155 Changbai St. Changping Dist., Beijing 102206, China.
authors.forename: Jialiang
authors.initials: J
authors.lastname: Du
authors.forename: Qing
authors.initials: Q
authors.lastname: Tang
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
authors.forename: Willoughby E
authors.initials: WE
authors.lastname: Rodney
authors.forename: Lihua
authors.initials: L
authors.lastname: Wang
authors.forename: Guodong
authors.initials: G
authors.lastname: Liang
mesh_terms: D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D017930:Genes, Reporter; D005818:Genetic Engineering; D005822:Genetic Vectors; D008156:Luciferases / Q000235:genetics / Q000378:metabolism*; D011819:Rabies Vaccines / Q000235:genetics*; D011820:Rabies virus / Q000235:genetics*; D011995:Recombination, Genetic; D014773:Virology / Q000379:methods
chemical_list: D011819:Rabies Vaccines; D008156:Luciferases