title: Expression of IL-17 and COX2 gene in peripheral blood leukocytes of vitiligo patients.
abstract: Vitiligo is a pigmentation disorder in which inflammatory mediators such as cytokines have a pivotal role in disease's pathogenesis. Interleukin 17 (IL-17A) is a proinflammatory cytokine which is involved in the induction of several proinflamatory mediators such as cyclooxygenase 2 (COX2). The aim of this study was to investigate the gene expression of IL-17 and COX2 in peripheral blood leukocytes of vitiligo's patients.Peripheral blood leukocytes from 15 patients with vitiligo and 15 healthy controls were separated using a gradient density centrifugation method. After total RNA isolation and cDNA synthesis, IL-17 and COX2 gene expression were quantified by real-time polymerase chain reaction (PCR). There were no significant differences in IL-17 and COX2 gene expression in lymphocytes of patients with vitiligo compared with control group (p<0.05). However there was an increased IL-17 and COX2 gene expression in neutrophils of patients compared to controls, but it did not reach statistical significance (p=0.05). We could not find any differences in IL-17 and Cox2 gene expression between clinical diseases subtypes, sex and age. There was a significant correlation between IL-17 and COX2 genes expression in the neutrophils of patients (p=0.00, r=0.80). Our results showed an increased expression in IL-17 and Cox-2 genes in neurophils of patients with vitiligo. This suggested that these two factors are involved in the inflammatory process. Further studies with a larger sample size might help to establish the role of these factors in the pathogenesis of diseases.
authors.affiliation: Rheumatic Disease Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Behnaz
authors.initials: B
authors.lastname: Esmaeili
authors.forename: Seyed Abdol Rahim
authors.initials: SA
authors.lastname: Rezaee
authors.forename: Pouran
authors.initials: P
authors.lastname: Layegh
authors.forename: Jalil
authors.initials: J
authors.lastname: Tavakkol Afshari
authors.forename: Phil
authors.initials: P
authors.lastname: Dye
authors.forename: Ehsan
authors.initials: E
authors.lastname: Ghayoor Karimiani
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Kalalinia
authors.forename: Houshang
authors.initials: H
authors.lastname: Rafatpanah
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D051546:Cyclooxygenase 2 / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D020381:Interleukin-17 / Q000235:genetics*; D007962:Leukocytes / Q000378:metabolism*; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D014820:Vitiligo / Q000209:etiology* / Q000378:metabolism
chemical_list: C508717:IL17A protein, human; D020381:Interleukin-17; D012333:RNA, Messenger; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human