title: Multiple pathways from three types of sugar receptor sites to metabotropic transduction pathways of the blowfly: study by the whole cell-clamp experiments.
abstract: Multiple pathways from three types of multiple receptor sites to three types of metabotropic signal transduction pathways were investigated in the whole cell-clamp experiments using isolated labellar sugar receptor neurons (cells) of the adult blowfly, Phormia regina. First, the concentration-response curves of three types of sweet taste components specialized to multiple receptor sites were obtained: sucrose for the pyranose sites (P-sites), fructose for the furanose sites (F-sites), and l-valine for the alkyl sites (R-sites). Next, the effects of inhibitors such as 2', 5'-dideoxyadenosine on adenylyl cyclase in the cAMP pathway, LY 83583 on guanylyl cyclase in the cGMP pathway, and U-73122 on phospholipase C in the IP₃ pathway were examined. The results showed that all of the inhibitors affected each specific target in the second-messenger transduction pathways. The obtained results verified that the P-site corresponded to the cAMP, the F-site to the cGMP, and the R-site to the IP₃ transduction pathway, and that these three signal pathways did not have crossing points.
authors.affiliation: Graduate School of Human Development and Environment, Kobe University; 3-11, Tsurukabuto, Nada, Kobe 657-8501, Japan.
authors.forename: Hideko
authors.initials: H
authors.lastname: Kan
authors.forename: Naoko
authors.initials: N
authors.lastname: Kataoka-Shirasugi
authors.forename: Taisaku
authors.initials: T
authors.lastname: Amakawa
mesh_terms: D000067956:Adenylyl Cyclase Inhibitors; D000634:Aminoquinolines / Q000494:pharmacology; D000818:Animals; D002628:Chemoreceptor Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D000242:Cyclic AMP / Q000502:physiology; D006152:Cyclic GMP / Q000502:physiology; D016048:Dideoxyadenosine / Q000031:analogs & derivatives / Q000494:pharmacology; D004175:Diptera / Q000187:drug effects* / Q000502:physiology; D004963:Estrenes / Q000494:pharmacology; D005632:Fructose / Q000494:pharmacology; D006162:Guanylate Cyclase / Q000037:antagonists & inhibitors; D018408:Patch-Clamp Techniques; D011760:Pyrrolidinones / Q000494:pharmacology; D015398:Signal Transduction; D013395:Sucrose / Q000494:pharmacology; D013649:Taste / Q000502:physiology*; D010738:Type C Phospholipases / Q000037:antagonists & inhibitors; D014633:Valine / Q000494:pharmacology
chemical_list: D000067956:Adenylyl Cyclase Inhibitors; D000634:Aminoquinolines; D004963:Estrenes; D011760:Pyrrolidinones; C060229:1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione; D005632:Fructose; D016048:Dideoxyadenosine; D013395:Sucrose; C041715:6-anilino-5,8-quinolinedione; C021008:2',5'-dideoxyadenosine; D000242:Cyclic AMP; D010738:Type C Phospholipases; D006162:Guanylate Cyclase; D006152:Cyclic GMP; D014633:Valine