title: The role of promoter methylation in Epstein-Barr virus (EBV) microRNA expression in EBV-infected B cell lines.
abstract: Epstein-Barr virus (EBV) microRNAs (miRNAs) are expressed in EBV-associated tumors and cell lines, but the regulation mechanism of their expression is unclear yet. We investigated whether the expression of EBV miRNAs is epigenetically regulated in EBV-infected B cell lines. The expression of BART miRNAs was inversely related with the methylation level of the BART promoter at both steady-state and following 5-aza-2'-deoxycytidine treatment of the cells. The expression of BHRF1 miRNAs also became detectable with the demethylation of Cp/Wp in latency I EBV-infected cell lines. Furthermore, in vitro methylation of the BART and Cp promoters reduced the promoter-driven transactivation. In contrast, tricostatin A had little effect on the expression of EBV miRNA expression as well as on the BART and Cp/Wp promoters. Our results suggest that promoter methylation, but not histone acetylation, plays a role in regulation of the EBV miRNA expression in EBV-infected B cell lines.
authors.affiliation: Research Institute of Immunobiology Department of Medical Lifescience College of Medicine The Catholic University of Korea Seoul.
authors.forename: Do Nyun
authors.initials: DN
authors.lastname: Kim
authors.forename: Yoon Jae
authors.initials: YJ
authors.lastname: Song
authors.forename: Suk Kyeong
authors.initials: SK
authors.lastname: Lee
mesh_terms: D001374:Azacitidine / Q000031:analogs & derivatives / Q000494:pharmacology; D001402:B-Lymphocytes / Q000378:metabolism / Q000821:virology; D002460:Cell Line; D019175:DNA Methylation; D015254:DNA Modification Methylases / Q000037:antagonists & inhibitors; D000077209:Decitabine; D015967:Gene Expression Regulation, Viral; D020868:Gene Silencing; D004854:Herpesvirus 4, Human / Q000235:genetics* / Q000502:physiology; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism*; D014764:Viral Proteins / Q000235:genetics
chemical_list: C083278:BHRF1 protein, Human herpesvirus 4; D035683:MicroRNAs; D012367:RNA, Viral; D014764:Viral Proteins; D000077209:Decitabine; D015254:DNA Modification Methylases; D001374:Azacitidine