title: Proteomics analysis of Bifidobacterium longum NCC2705 growing on glucose, fructose, mannose, xylose, ribose, and galactose.
abstract: To investigate the molecular mechanisms underlying carbohydrate uptake and connected metabolic pathways of Bifidobacterium longum NCC2705, the proteomic profiles of bacteria grown on different carbon sources including glucose, fructose, mannose, xylose, ribose, and galactose were analyzed. Our results show that all sugars tested were catabolized via the bifid shunt. Sixty-eight proteins that exhibited changes in abundance of threefold or greater were identified by MS. A striking observation was the differential expression of proteins related to the pyruvate metabolism. Further analysis of acetic acid and lactic acid in the culture supernatants by HPLC at the end of fermentation showed that more lactic acid was produced during growth on fructose, ribose, xylose, galactose and more acetic acid was produced during the fermentation of glucose and mannose. Growth experiments revealed that B. longum NCC2705 preferentially used fructose, ribose, xylose, and galactose with higher growth rates over glucose and mannose. Furthermore, five proteins (GroEL, Eno, Tal, Pgm, and BL0033) exhibited clear phosphorylation modifications at serine and/or tyrosine residues. BL0033, a component of an ATP-binding cassette (ABC) transporter, was significantly more abundant in bacteria grown on fructose and, to a lesser extent, ribose and xylose. RT-PCR analysis revealed that all genes of the ABC transporter are induced in the presence of these sugars suggesting that BL0033, BL0034, BL0035, and BL0036 constitute an ABC transporter with fructose as preferred substrate.
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, P. R. China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Liu
authors.forename: Shuai
authors.initials: S
authors.lastname: Wang
authors.forename: Bin
authors.initials: B
authors.lastname: Xu
authors.forename: Yanghong
authors.initials: Y
authors.lastname: Guo
authors.forename: Jiangli
authors.initials: J
authors.lastname: Zhao
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Zhongke
authors.initials: Z
authors.lastname: Sun
authors.forename: Changlin
authors.initials: C
authors.lastname: Shao
authors.forename: Xiao
authors.initials: X
authors.lastname: Wei
authors.forename: Zheng
authors.initials: Z
authors.lastname: Jiang
authors.forename: Xuesong
authors.initials: X
authors.lastname: Wang
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.forename: Liuyu
authors.initials: L
authors.lastname: Huang
authors.forename: Darong
authors.initials: D
authors.lastname: Hu
authors.forename: Xiang
authors.initials: X
authors.lastname: He
authors.forename: Christian U
authors.initials: CU
authors.lastname: Riedel
authors.forename: Jing
authors.initials: J
authors.lastname: Yuan
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D001644:Bifidobacterium / Q000737:chemistry* / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D002244:Carbon / Q000737:chemistry / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D005632:Fructose / Q000378:metabolism*; D005690:Galactose / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D008358:Mannose / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D011773:Pyruvates / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D014994:Xylose / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D011773:Pyruvates; D005632:Fructose; D002244:Carbon; D014994:Xylose; D005947:Glucose; D008358:Mannose; D005690:Galactose