title: Purification and characterization of a soluble cytochrome c capable of delivering electrons to chlorate reductase in Ideonella dechloratans.
abstract: The electron donor for periplasmic chlorate reductase of Ideonella dechloratans has been suggested to be a soluble cytochrome c. We describe here the purification of the 9-kDa periplasmic cytochrome c, denoted cytochrome c-Id1, and demonstrate its ability to serve as an electron donor for purified chlorate reductase. The reaction rate was found to be linearly dependent on the cytochrome c concentration in the range of 0.6-4 μM. A route for electron transport involving a soluble cytochrome c is similar to that found for other periplasmic oxidoreductases of the dimethyl sulfoxide reductase family, but different from that suggested for the (per)chlorate reductase of Dechloromonas species.
authors.affiliation: Department of Chemistry and Biomedical Sciences, Faculty of Technology and Science, Karlstad University, Sweden.
authors.forename: Anna S
authors.initials: AS
authors.lastname: Bäcklund
authors.forename: Thomas
authors.initials: T
authors.lastname: Nilsson
mesh_terms: D020562:Betaproteobacteria / Q000737:chemistry / Q000201:enzymology*; D045304:Cytochromes c / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D004579:Electron Transport; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D010088:Oxidoreductases / Q000737:chemistry* / Q000378:metabolism*; D013053:Spectrophotometry
chemical_list: D045304:Cytochromes c; D010088:Oxidoreductases; C021777:chlorate reductase