title: Preparative separation of high-purity cordycepin from Cordyceps militaris(L.) Link by high-speed countercurrent chromatography.
abstract: A high-speed counter-current chromatography (HSCCC) technique in a preparative scale has been applied to separate and purify cordycepin from the extract of Cordyceps militaris(L.) Link by a one-step separation. A high efficiency of HSCCC separation was achieved on a two-phase solvent system of n-hexane-n-butanol-methanol-water (23:80:30:155, v/v/v/v) by eluting the lower mobile phase at a flow rate of 2 ml/min under a revolution speed of 850 rpm. HSCCC separation of 216.2 mg crude sample (contained cordycepin at 44.7% purity after 732 cation-exchange resin clean-up) yielded 64.8 mg cordycepin with purity of 98.9% and 91.7% recovery. Identification of the target compound was performed by UV, IR, MS, (1)H NMR and (13)C NMR.
authors.affiliation: School of Chemistry and Environment, South China Normal University, Guangzhou 510631, China.
authors.forename: Licai
authors.initials: L
authors.lastname: Zhu
authors.forename: Yong
authors.initials: Y
authors.lastname: Liang
authors.forename: Deqiang
authors.initials: D
authors.lastname: Lao
authors.forename: Tianyou
authors.initials: T
authors.lastname: Zhang
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Ito