title: Effect of cyclosporine pretreatment on mitochondrial function in vitrified bovine mature oocytes.
abstract: Vitrification had a significantly negative impact on the mitochondrial function of bovine oocytes. However, 40 μg/mL cyclosporine pretreatment before vitrification contributed greatly to maintaining mitochondrial membrane potential and adenosine triphosphate content, decreasing reactive oxygen species level, and thereby increasing the developmental ability of vitrified oocytes.
authors.affiliation: Embryo Biotechnology and Reproduction Laboratory, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, People's Republic of China.
authors.forename: Xue-Ming
authors.initials: XM
authors.lastname: Zhao
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Du
authors.forename: Dong
authors.initials: D
authors.lastname: Wang
authors.forename: Hai-Sheng
authors.initials: HS
authors.lastname: Hao
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Tong
authors.initials: T
authors.lastname: Qin
authors.forename: Hua-Bin
authors.initials: HB
authors.lastname: Zhu
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001755:Blastocyst / Q000187:drug effects; D000001:Calcimycin / Q000494:pharmacology; D002417:Cattle; D015925:Cryopreservation; D016572:Cyclosporine / Q000494:pharmacology*; D046149:Embryo Culture Techniques; D005260:Female; D007476:Ionophores / Q000494:pharmacology; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D009865:Oocytes / Q000187:drug effects* / Q000378:metabolism; D010312:Parthenogenesis / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D013997:Time Factors; D058989:Vitrification
chemical_list: D007476:Ionophores; D017382:Reactive Oxygen Species; D000001:Calcimycin; C001020:N(6),N(6)-dimethyladenine; D016572:Cyclosporine; D000255:Adenosine Triphosphate; D000225:Adenine