title: Shotgun proteomic analysis of the unicellular alga Ostreococcus tauri.
abstract: Ostreococcus tauri is a unicellular green alga and amongst the smallest and simplest free-living eukaryotes. The O. tauri genome sequence was determined in 2006. Molecular, physiological and taxonomic data that has been generated since then highlight its potential as a simple model species for algae and plants. However, its proteome remains largely unexplored. This paper describes the global proteomic study of O. tauri, using mass spectrometry-based approaches: phosphopeptide enrichment, cellular fractionation, label-free quantification and (15)N metabolic labeling. The O. tauri proteome was analyzed under the following conditions: sampling at different times during the circadian cycle, after 24h of illumination, after 24h of darkness and under various nitrogen source supply levels. Cell cycle related proteins such as dynamin and kinesin were significantly up-regulated during the daylight-to-darkness transition. This is reflected by their higher intensity at ZT13 and this transition phase coincides with the end of mitosis. Proteins involved in several metabolic mechanisms were found to be up-regulated under low nitrogen conditions, including carbon storage pathways, glycolysis, phosphate transport, and the synthesis of inorganic polyphosphates. Ostreococcus tauri responds to low nitrogen conditions by reducing its nitrogen assimilation machinery which suggests an atypical adaptation mechanism for coping with a nutrient-limited environment.
authors.affiliation: Centre for Systems Biology at Edinburgh, The University of Edinburgh, Edinburgh, UK. thierry.lebihan@ed.ac.uk
authors.forename: Thierry
authors.initials: T
authors.lastname: Le Bihan
authors.forename: Sarah F
authors.initials: SF
authors.lastname: Martin
authors.forename: Eliane S
authors.initials: ES
authors.lastname: Chirnside
authors.forename: Gerben
authors.initials: G
authors.lastname: van Ooijen
authors.forename: Martin E
authors.initials: ME
authors.lastname: Barrios-Llerena
authors.forename: John S
authors.initials: JS
authors.lastname: O'Neill
authors.forename: Pavel V
authors.initials: PV
authors.lastname: Shliaha
authors.forename: Lorraine E
authors.initials: LE
authors.lastname: Kerr
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Millar
mesh_terms: D000460:Chlorophyta / Q000737:chemistry* / Q000378:metabolism; D002940:Circadian Rhythm; D010748:Phosphopeptides / Q000032:analysis; D010940:Plant Proteins / Q000032:analysis*; D040901:Proteomics / Q000379:methods*
chemical_list: D010748:Phosphopeptides; D010940:Plant Proteins