title: BMP-2 and ALP gene expression induced by a BMP-2 gene-fibronectin-apatite composite layer.
abstract: The bone morphogenetic protein 2 (BMP-2) gene delivery system with a gene-fibronectin (Fn)-apatite composite layer was fabricated on the surface of a hydroxyapatite ceramic scaffold. The BMP-2 gene-Fn-apatite composite layer was coated on the scaffold using a supersaturated calcium phosphate solution supplemented with BMP-2 DNA and Fn. The scaffolds were ectopically implanted into the dorsal subcutaneous tissue of rats. Four weeks after the implantation, the hydroxyapatite scaffold coated with the BMP-2 gene-Fn-apatite composite layer showed improved gene expressions of BMP-2 and alkaline phosphatase as compared with the scaffold coated with the apatite layer. Although these results suggest the possibility of ectopic bone formation induced by the present gene delivery system, further study is necessary to prove this.
authors.affiliation: Human Technology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan. xp-wang@aist.go.jp
authors.forename: Xiupeng
authors.initials: X
authors.lastname: Wang
authors.forename: Ayako
authors.initials: A
authors.lastname: Oyane
authors.forename: Hideo
authors.initials: H
authors.lastname: Tsurushima
authors.forename: Yu
authors.initials: Y
authors.lastname: Sogo
authors.forename: Xia
authors.initials: X
authors.lastname: Li
authors.forename: Atsuo
authors.initials: A
authors.lastname: Ito
mesh_terms: D000469:Alkaline Phosphatase / Q000096:biosynthesis* / Q000737:chemistry; D000818:Animals; D001031:Apatites; D015415:Biomarkers / Q000378:metabolism; D055396:Bone Morphogenetic Protein 2 / Q000096:biosynthesis* / Q000737:chemistry; D004247:DNA / Q000737:chemistry; D017886:Durapatite; D005353:Fibronectins / Q000737:chemistry*; D005786:Gene Expression Regulation; D018014:Gene Transfer Techniques; D008853:Microscopy / Q000379:methods; D008855:Microscopy, Electron, Scanning / Q000379:methods; D051381:Rats; D011916:Rats, Inbred F344; D013499:Surface Properties
chemical_list: D001031:Apatites; D015415:Biomarkers; D055396:Bone Morphogenetic Protein 2; D005353:Fibronectins; D004247:DNA; D017886:Durapatite; D000469:Alkaline Phosphatase