title: [Operating conditions for the rapid mutation of the oleaginous yeast by atmospheric and room temperature plasmas and the characteristics of the mutants].
abstract: To obtain oleaginous yeast mutants with improved lipid production and growth rates, an atmospheric and room temperature plasma (ARTP) jet was used with a 96-well plate for high throughput screening. Mutants with changes in growth rates and lipid contents were obtained. At a lethality rate of 99%, the positive mutation rate of the yeast cells was 27.2% evaluated by the growth rates of the mutants and the comparison with the wild strain. The fermentation in a medium composed of yeast extract (10 g/L), peptone (10 g/L) and D-glucose (20 g/L) resulted in the lipid yield of the mutant (C4) with 4.07% (W/W) compared with that of the wild strain (1.87%).
authors.affiliation: Department of Chemical Engineering, Tsinghua University, Beijing 100084, China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Jin
authors.forename: Mingyue
authors.initials: M
authors.lastname: Fang
authors.forename: Chong
authors.initials: C
authors.lastname: Zhang
authors.forename: Peixia
authors.initials: P
authors.lastname: Jiang
authors.forename: Nan
authors.initials: N
authors.lastname: Ge
authors.forename: Heping
authors.initials: H
authors.lastname: Li
authors.forename: Xinhui
authors.initials: X
authors.lastname: Xing
authors.forename: Chengyu
authors.initials: C
authors.lastname: Bao
mesh_terms: D001272:Atmosphere; D056804:Biofuels; D003470:Culture Media; D005285:Fermentation; D005947:Glucose / Q000494:pharmacology; D008055:Lipids / Q000096:biosynthesis*; D009154:Mutation; D010461:Peptones / Q000494:pharmacology; D013696:Temperature; D015003:Yeasts / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*
chemical_list: D056804:Biofuels; D003470:Culture Media; D008055:Lipids; D010461:Peptones; D005947:Glucose