title: Cellular transcriptional profiling in human lung epithelial cells infected by different subtypes of influenza A viruses reveals an overall down-regulation of the host p53 pathway.
abstract: BACKGROUND
  Influenza viruses can modulate and hijack several cellular signalling pathways to efficiently support their replication. We recently investigated and compared the cellular gene expression profiles of human lung A549 cells infected by five different subtypes of human and avian influenza viruses (Josset et al. Plos One 2010). Using these transcriptomic data, we have focused our analysis on the modulation of the p53 pathway in response to influenza infection.
  
  
  RESULTS
  Our results were supported by both RT-qPCR and western blot analyses and reveal multiple alterations of the p53 pathway during infection. A down-regulation of mRNA expression was observed for the main regulators of p53 protein stability during infection by the complete set of viruses tested, and a significant decrease in p53 mRNA expression was also observed in H5N1 infected cells. In addition, several p53 target genes were also down-regulated by these influenza viruses and the expression of their product reduced.
  
  
  CONCLUSIONS
  Our data reveal that influenza viruses cause an overall down-regulation of the host p53 pathway and highlight this pathway and p53 protein itself as important viral targets in the altering of apoptotic processes and in cell-cycle regulation.
authors.affiliation: Laboratoire de Virologie et Pathologie Humaine VirPath, Université Claude Bernard Lyon 1, Université de Lyon, Lyon, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Terrier
authors.forename: Laurence
authors.initials: L
authors.lastname: Josset
authors.forename: Julien
authors.initials: J
authors.lastname: Textoris
authors.forename: Virginie
authors.initials: V
authors.lastname: Marcel
authors.forename: Gaëlle
authors.initials: G
authors.lastname: Cartet
authors.forename: Olivier
authors.initials: O
authors.lastname: Ferraris
authors.forename: Catherine
authors.initials: C
authors.lastname: N'guyen
authors.forename: Bruno
authors.initials: B
authors.lastname: Lina
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Diaz
authors.forename: Jean-Christophe
authors.initials: JC
authors.lastname: Bourdon
authors.forename: Manuel
authors.initials: M
authors.lastname: Rosa-Calatrava
mesh_terms: D015153:Blotting, Western; D002460:Cell Line; D015536:Down-Regulation; D004847:Epithelial Cells / Q000276:immunology* / Q000821:virology*; D020869:Gene Expression Profiling; D006801:Humans; D009980:Influenza A virus / Q000276:immunology* / Q000472:pathogenicity*; D007251:Influenza, Human / Q000276:immunology / Q000473:pathology* / Q000821:virology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis*
chemical_list: D016159:Tumor Suppressor Protein p53