title: A simple, sensitive, rapid and specific detection method for Bisphenol A based on Fluorescence Polarization Immunoassay.
abstract: A rapid, sensitive, specific and simple method based on Fluorescence Polarization Immunoassay (FPIA) was developed for the quantitative detection of Bisphenol A in water. 4, 4-Bis (4-hydroxyphenyl) valeric acid (BHPVA) was selected as the hapten to produce the immunogen. Fluorescein-labeled Bisphenol A derivatives (tracers) with different structures were synthesized and purified by thin layer chromatography. Based on the polyclonal antibody and tracers, an optimized FPIA method was developed with a detection limit (10% inhibition) of 2 μg L(-1) and a linear working range of 20 to 800 μg L(-1). FPIA was suitable for screening a large number of samples. The recoveries of fortified Tai lake water samples ranged from 91.85 to 102.78%, and tap water samples ranged from 90.36 to 96.01%. The coefficients of variation were all less than 20%. This FPIA method, which did not require a complicated cleanup process, proved to be very useful for the screening of Bisphenol A in environmental water samples.
authors.affiliation: School of Food Science & Technology, State Key Lab of Food Science & Technology, Jiangnan University, Wuxi, China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Wu
authors.forename: Libing
authors.initials: L
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Ma
authors.forename: Yingyue
authors.initials: Y
authors.lastname: Zhu
authors.forename: Liguang
authors.initials: L
authors.lastname: Xu
authors.forename: Hua
authors.initials: H
authors.lastname: Kuang
authors.forename: Chuanlai
authors.initials: C
authors.lastname: Xu
mesh_terms: D000395:Air Pollutants, Occupational / Q000009:adverse effects / Q000032:analysis*; D001559:Benzhydryl Compounds; D004326:Drinking; D016231:Fluorescence Polarization Immunoassay; D057166:High-Throughput Screening Assays; D006801:Humans; D060106:Lakes / Q000737:chemistry; D009787:Occupational Medicine / Q000379:methods; D010636:Phenols / Q000009:adverse effects / Q000032:analysis / Q000138:chemical synthesis*; D012680:Sensitivity and Specificity; D012996:Solutions / Q000737:chemistry*; D000098826:Bisphenol A Compounds
chemical_list: D000395:Air Pollutants, Occupational; D001559:Benzhydryl Compounds; D010636:Phenols; D012996:Solutions; C006780:bisphenol A; D000098826:Bisphenol A Compounds