title: Invariant NKT cells are required for airway inflammation induced by environmental antigens.
abstract: Invariant NKT cells (iNKT cells) are a unique subset of T lymphocytes that rapidly carry out effector functions. In this study, we report that a majority of sterile house dust extracts (HDEs) tested contained antigens capable of activating mouse and human iNKT cells. HDEs had adjuvant-like properties in an ovalbumin (OVA)-induced asthma model, which were dependent on Vα14i NKT cells, as vaccinated animals deficient for iNKT cells displayed significantly attenuated immune responses and airway inflammation. Furthermore, the administration of HDEs together with OVA mutually augmented the synthesis of cytokines by Vα14i NKT cells and by conventional CD4(+) T cells in the lung, demonstrating a profound immune response synergy for both Th2 cytokines and IL-17A. These data demonstrate that iNKT cell antigens are far more widely dispersed in the environment than previously anticipated. Furthermore, as the antigenic activity in different houses varied greatly, they further suggest that iNKT cell responses to ambient antigens, particular to certain environments, might promote sensitization to conventional respiratory allergens.
authors.affiliation: Division of Developmental Immunology, La Jolla Institute for Allergy and Immunology, La Jolla, CA 92037, USA.
authors.forename: Gerhard
authors.initials: G
authors.lastname: Wingender
authors.forename: Paul
authors.initials: P
authors.lastname: Rogers
authors.forename: Glenda
authors.initials: G
authors.lastname: Batzer
authors.forename: Myung Steve
authors.initials: MS
authors.lastname: Lee
authors.forename: Dong
authors.initials: D
authors.lastname: Bai
authors.forename: Bo
authors.initials: B
authors.lastname: Pei
authors.forename: Archana
authors.initials: A
authors.lastname: Khurana
authors.forename: Mitchell
authors.initials: M
authors.lastname: Kronenberg
authors.forename: Anthony A
authors.initials: AA
authors.lastname: Horner
mesh_terms: D000485:Allergens / Q000494:pharmacology*; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000166:cytology; D004777:Environment; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism; D006801:Humans; D006825:Hybridomas / Q000378:metabolism; D007249:Inflammation; D020381:Interleukin-17 / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D055611:Natural Killer T-Cells / Q000166:cytology*; D010047:Ovalbumin / Q000378:metabolism; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism; D018418:Th2 Cells / Q000166:cytology
chemical_list: D000485:Allergens; D016207:Cytokines; D020381:Interleukin-17; D011948:Receptors, Antigen, T-Cell; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D010047:Ovalbumin