title: Fucoidan from Sargassum sp. and Fucus vesiculosus reduces cell viability of lung carcinoma and melanoma cells in vitro and activates natural killer cells in mice in vivo.
abstract: Fucoidan is known to exhibit crucial biological activities, including anti-tumor activity. In this study, we examined the influence of crude fucoidan extracted from Sargassum sp. (MTA) and Fucus vesiculosus (SIG) on Lewis lung carcinoma cells (LCC) and melanoma B16 cells (MC). In vitro studies were performed using cell viability analysis and showed that SIG and MTA fucoidans significantly decreased the viable number of LCC and MC cells in a dose-response fashion. Histochemical staining showed morphological changes of melanoma B16 cells after exposure to fucoidan. The observed changes were indicative of crude fucoidan induced apoptosis. Male C57BL/6JJCL mice were subjected to daily i.p. injections over 4 days with either SIG or MTA fucoidan (50mg/kg body wt.). The cytolytic activity of natural killer (NK) cells was enhanced by crude fucoidan in a dose-dependent manner as indicated by (51)Cr labeled YAC-1 target cell release. This study provides substantial indications that crude fucoidan exerts bioactive effects on lung and skin cancer model cells in vitro and induces enhanced natural killer cell activity in mice in vivo.
authors.affiliation: Department of Chemical and Biochemical Engineering, Technical University of Denmark, Lyngby, Denmark.
authors.forename: Marcel Tutor
authors.initials: MT
authors.lastname: Ale
authors.forename: Hiroko
authors.initials: H
authors.lastname: Maruyama
authors.forename: Hidekazu
authors.initials: H
authors.lastname: Tamauchi
authors.forename: Jørn D
authors.initials: JD
authors.lastname: Mikkelsen
authors.forename: Anne S
authors.initials: AS
authors.lastname: Meyer
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D018827:Carcinoma, Lewis Lung / Q000276:immunology / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D045424:Complex Mixtures / Q000302:isolation & purification / Q000494:pharmacology; D044645:Fucus / Q000737:chemistry*; D007694:Killer Cells, Natural / Q000187:drug effects* / Q000276:immunology; D008175:Lung Neoplasms / Q000276:immunology / Q000473:pathology*; D008297:Male; D008545:Melanoma / Q000276:immunology / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D044647:Sargassum / Q000737:chemistry*; D012878:Skin Neoplasms / Q000276:immunology / Q000473:pathology
chemical_list: D000970:Antineoplastic Agents; D045424:Complex Mixtures; D011134:Polysaccharides; C007789:fucoidan