title: The relative efficacy of different strain combinations of lactic acid bacteria in the reduction of populations of Salmonella enterica Typhimurium in the livers and spleens of mice.
abstract: Multispecies probiotics have been reported to be more effective than monostrain probiotics in health promoting for the host. In this study, 12 lactic acid bacteria (LAB) strains were selected based on the level of induction of tumor necrosis factor (TNF)-α in RAW 264.7 macrophage cells. Their adherence to Caco-2 cells and inhibitory effects on Salmonella invasion of Caco-2 cells were compared. Strains with different probiotic properties were then combined and BALB/c mice were fed with LAB strains for 63 days; then the mice were challenged with Salmonella on day 64. For Salmonella-unchallenged mice that received a multistrain combination of LAB strains that have greater TNF-α production in macrophages, greater adherence and inhibit Salmonella invasion of Caco-2 cells to a greater extent, their peritoneal macrophages had greater phagocytic activity. For Salmonella-challenged mice, a significant reduction of Salmonella cells in the livers and spleens of the mice was observed 8 days post challenge. The addition of 12% skim milk powder together with LAB strain combinations significantly enhanced the reduction of Salmonella cells in the mice livers and spleens. In conclusion, we have shown that LAB strain combinations with particular probiotic properties when fed to mice can inhibit Salmonella invasion of the liver and spleen.
authors.affiliation: Department of Food Science and Technology, Hung Kuang University, Taichung, Taiwan, ROC.
authors.forename: Cheng-Chih
authors.initials: CC
authors.lastname: Tsai
authors.forename: Hsin-Wen
authors.initials: HW
authors.lastname: Liang
authors.forename: Bi
authors.initials: B
authors.lastname: Yu
authors.forename: Chang-Chi
authors.initials: CC
authors.lastname: Hsieh
authors.forename: Chin-Fa
authors.initials: CF
authors.lastname: Hwang
authors.forename: Ming-Hui
authors.initials: MH
authors.lastname: Chen
authors.forename: Hau-Yang
authors.initials: HY
authors.lastname: Tsen
mesh_terms: D000818:Animals; D000898:Antibiosis; D001422:Bacterial Adhesion; D002460:Cell Line; D004847:Epithelial Cells; D006094:Gram-Positive Bacteria / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D019344:Lactic Acid / Q000378:metabolism; D008099:Liver / Q000382:microbiology*; D008264:Macrophages / Q000382:microbiology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D019936:Probiotics / Q000494:pharmacology*; D012481:Salmonella Infections, Animal / Q000382:microbiology*; D012486:Salmonella typhimurium / Q000302:isolation & purification / Q000472:pathogenicity*; D013154:Spleen / Q000382:microbiology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D014409:Tumor Necrosis Factor-alpha; D019344:Lactic Acid