title: Surface-enhanced laser desorption ionization/time-of-flight (SELDI-TOF) mass spectrometry (MS) as a phenotypic method for rapid identification of antibiotic resistance.
abstract: Based on experiments with 10 defined strains of Escherichia coli, we present a new method for bacterial phenotyping using SELDI-TOF mass spectrometry. Changes in bacterial protein profiles in the context of the time of cultivation and the antibiotic environment were minimal. Proteom subprofiling may further distinguish between strains with specific susceptibility to antimicrobials. Mass spec-based methods may become common in the future of bacterial pathogen identification in clinical microbiology diagnostics.
authors.affiliation: Department of Laboratory Medicine, Masaryk Memorial Cancer Institute, Zluty kopec 7, 656 53 Brno, Czech Republic. dubska@mou.cz
authors.forename: Lenka
authors.initials: L
authors.lastname: Dubska
authors.forename: Katerina
authors.initials: K
authors.lastname: Pilatova
authors.forename: Monika
authors.initials: M
authors.lastname: Dolejska
authors.forename: Zbynek
authors.initials: Z
authors.lastname: Bortlicek
authors.forename: Tereza
authors.initials: T
authors.lastname: Frostova
authors.forename: Ivan
authors.initials: I
authors.lastname: Literak
authors.forename: Dalibor
authors.initials: D
authors.lastname: Valik
mesh_terms: D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000737:chemistry*; D029968:Escherichia coli Proteins / Q000032:analysis*; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D020543:Proteome / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D029968:Escherichia coli Proteins; D020543:Proteome