title: Resolution of quadruplex polymorphism by size-exclusion chromatography.
abstract: This unit describes a method for separation of quadruplex species formed from the same sequence via size-exclusion chromatography (SEC). Polymorphism is inherent to quadruplex formation, and even relatively simple quadruplex-forming sequences, such as the human telomere sequence d(GGG(TTAGGG)(3)), can form a myriad of possible configurations. HPLC, especially using reversed-phase and anion-exchange methods, has been a mainstay of nucleic acids research and purification for many decades. These methods have been applied for separation of individual quadruplex species formed in a mixture from the same parent sequence.
authors.affiliation: James Graham Brown Cancer Center, University of Louisville, Louisville, Kentucky, USA.
authors.forename: M Clarke
authors.initials: MC
authors.lastname: Miller
authors.forename: John O
authors.initials: JO
authors.lastname: Trent
mesh_terms: D002850:Chromatography, Gel / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D054856:G-Quadruplexes; D006801:Humans; D011110:Polymorphism, Genetic; D016615:Telomere / Q000737:chemistry / Q000235:genetics*