title: Probing mechanical principles of focal contacts in cell-matrix adhesion with a coupled stochastic-elastic modelling framework.
abstract: Cell-matrix adhesion depends on the collective behaviours of clusters of receptor-ligand bonds called focal contacts between cell and extracellular matrix. While the behaviour of a single molecular bond is governed by statistical mechanics at the molecular scale, continuum mechanics should be valid at a larger scale. This paper presents an overview of a series of recent theoretical studies aimed at probing the basic mechanical principles of focal contacts in cell-matrix adhesion via stochastic-elastic models in which stochastic descriptions of molecular bonds and elastic descriptions of interfacial traction-separation are unified in a single modelling framework. The intention here is to illustrate these principles using simple analytical and numerical models. The aim of the discussions is to provide possible clues to the following questions: why does the size of focal adhesions (FAs) fall into a narrow range around the micrometre scale? How can cells sense and respond to substrates of varied stiffness via FAs? How do the magnitude and orientation of mechanical forces affect the binding dynamics of FAs? The effects of cluster size, cell-matrix elastic modulus, loading direction and cytoskeletal pretension on the lifetime of FA clusters have been investigated by theoretical arguments as well as Monte Carlo numerical simulations, with results showing that intermediate adhesion size, stiff substrate, cytoskeleton stiffening, low-angle pulling and moderate cytoskeletal pretension are factors that contribute to stable FAs. From a mechanistic point of view, these results provide possible explanations for a wide range of experimental observations and suggest multiple mechanisms by which cells can actively control adhesion and de-adhesion via cytoskeletal contractile machinery in response to mechanical properties of their surroundings.
authors.affiliation: School of Engineering, Brown University, Providence, RI 02912, USA. huajian_gao@brown.edu
authors.forename: Huajian
authors.initials: H
authors.lastname: Gao
authors.forename: Jin
authors.initials: J
authors.lastname: Qian
authors.forename: Bin
authors.initials: B
authors.lastname: Chen
mesh_terms: D002448:Cell Adhesion / Q000502:physiology*; D003198:Computer Simulation; D003599:Cytoskeleton / Q000502:physiology*; D005109:Extracellular Matrix / Q000502:physiology*; D022001:Focal Adhesions / Q000502:physiology*; D008954:Models, Biological; D009010:Monte Carlo Method; D013269:Stochastic Processes