title: ALTERED MERISTEM PROGRAM 1 is involved in development of seed dormancy in Arabidopsis.
abstract: Mutants in the rice PLASTOCHRON 3 and maize VIVIPAROUS 8 genes have been shown to have reduced dormancy and ABA levels. In this study we used several mutants in the orthologous gene ALTERED MERISTEM PROGRAM 1 (AMP1) to determine its role in seed dormancy in Arabidopsis. Here we report that there are accession-specific effects of mutations in AMP1. In one accession, amp1 mutants produce seeds with higher dormancy, while those in two other accessions produce seeds of lower dormancy. These accession-specific effects of mutating AMP1 were shown to extend to ABA levels. We assayed global gene transcription differences in seeds of wild-type and mutant from two accessions demonstrating opposing phenotypes. The transcript changes observed indicate that the amp1 mutation shifts the seed transcriptome from a dormant into an after-ripened state. Specific changes in gene expression in the mutants give insight into the direct and indirect effects that may be contributing to the opposing dormancy phenotypes observed, and reveal a role for AMP1 in the acquisition and/or maintenance of seed dormancy in Arabidopsis.
authors.affiliation: Division of Plant Industry, CSIRO, Canberra, Australian Capital Territory, Australia.
authors.forename: Jayne
authors.initials: J
authors.lastname: Griffiths
authors.forename: Jose M
authors.initials: JM
authors.lastname: Barrero
authors.forename: Jennifer
authors.initials: J
authors.lastname: Taylor
authors.forename: Chris A
authors.initials: CA
authors.lastname: Helliwell
authors.forename: Frank
authors.initials: F
authors.lastname: Gubler
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism; D000483:Alleles; D017360:Arabidopsis / Q000235:genetics / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002268:Carboxypeptidases / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics; D009154:Mutation / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D057445:Plant Dormancy / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000235:genetics / Q000254:growth & development
chemical_list: D029681:Arabidopsis Proteins; D012333:RNA, Messenger; D000040:Abscisic Acid; D002268:Carboxypeptidases; C506681:AMP1 protein, Arabidopsis