title: Involvement of LOC66273 isoform 2, a novel Mth938 containing protein, in MAPK pathway.
abstract: Using dual-luciferase reporter assay system, our previous study showed that LI2, significantly increased AP-1 transcriptional activity. Sub-cellular localization showed that GFP-LI2 fusion protein is diffusely distributed in the cytoplasm, with some highly concentrated spots around the nucleus, suggesting that LI2 protein has a physiological role in cytoplasm. Overexpression of LI2 significantly increased AP-1 transcriptional activity. Moreover, LI2 significantly promoted transcriptional activity of Elk1 and c-jun, which might, at least partly, be associated with activated ERK1/2 and JNK/SAPK signaling pathway. These data suggest that LI2 is a novel MAPK regulating protein.
authors.affiliation: State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing, China.
authors.forename: Hualang
authors.initials: H
authors.lastname: Wang
authors.forename: Xi
authors.initials: X
authors.lastname: Ma
authors.forename: Heyu
authors.initials: H
authors.lastname: Zhang
authors.forename: Jianjun
authors.initials: J
authors.lastname: Zang
authors.forename: Shengdi
authors.initials: S
authors.lastname: Hu
authors.forename: Defa
authors.initials: D
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D017931:DNA Primers; D018076:DNA, Complementary; D020869:Gene Expression Profiling; D006801:Humans; D020935:MAP Kinase Signaling System; D051379:Mice; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary