title: Growth-rate dependence reveals design principles of plasmid copy number control.
abstract: Genetic circuits in bacteria are intimately coupled to the cellular growth rate as many parameters of gene expression are growth-rate dependent. Growth-rate dependence can be particularly pronounced for genes on plasmids; therefore the native regulatory systems of a plasmid such as its replication control system are characterized by growth-rate dependent parameters and regulator concentrations. This natural growth-rate dependent variation of regulator concentrations can be used for a quantitative analysis of the design of such regulatory systems. Here we analyze the growth-rate dependence of parameters of the copy number control system of ColE1-type plasmids in E. coli. This analysis allows us to infer the form of the control function and suggests that the Rom protein increases the sensitivity of control.
authors.affiliation: Max Planck Institute of Colloids and Interfaces, Potsdam, Germany. klumpp@mpikg.mpg.de
authors.forename: Stefan
authors.initials: S
authors.lastname: Klumpp
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development*; D018628:Gene Dosage / Q000235:genetics*; D010957:Plasmids / Q000235:genetics*; D017384:Sequence Deletion
chemical_list: D001426:Bacterial Proteins; C042833:ROM protein, Bacteria