title: Molecular cloning and analysis of a DNA repetitive element from the mouse genome.
abstract: We report the development of a 3-week laboratory activity for an undergraduate molecular biology course. This activity introduces students to the practice of basic molecular techniques such as restriction enzyme digestion, agarose gel electrophoresis, cloning, plasmid DNA purification, Southern blotting, and sequencing. Students learn how to carry out a GenBank™ search as they are encouraged to compile most available information about their cloned sequence. The natural integration of the bench work with the use of the bioinformatics tools is considered a major advantage of this laboratory course.
authors.affiliation: Unidad Asociada Biología Molecular, Facultad de Ciencias, 11600 Montevideo; Departamento de Biología Molecular, Instituto de Investigaciones Biológicas Clemente Estable, 11600 Montevideo, Uruguay. geisinge@iibce.edu.uy.
authors.forename: Adriana
authors.initials: A
authors.lastname: Geisinger
authors.forename: Gabriela
authors.initials: G
authors.lastname: Cossio
authors.forename: Rodolfo
authors.initials: R
authors.lastname: Wettstein