title: Mitotic spindle proteomics in Chinese hamster ovary cells.
abstract: Mitosis is a fundamental process in the development of all organisms. The mitotic spindle guides the cell through mitosis as it mediates the segregation of chromosomes, the orientation of the cleavage furrow, and the progression of cell division. Birth defects and tissue-specific cancers often result from abnormalities in mitotic events. Here, we report a proteomic study of the mitotic spindle from Chinese Hamster Ovary (CHO) cells. Four different isolations of metaphase spindles were subjected to Multi-dimensional Protein Identification Technology (MudPIT) analysis and tandem mass spectrometry. We identified 1155 proteins and used Gene Ontology (GO) analysis to categorize proteins into cellular component groups. We then compared our data to the previously published CHO midbody proteome and identified proteins that are unique to the CHO spindle. Our data represent the first mitotic spindle proteome in CHO cells, which augments the list of mitotic spindle components from mammalian cells.
authors.affiliation: Department of Genetics, University of Wisconsin-Madison, Madison, Wisconsin, United States of America.
authors.forename: Mary Kate
authors.initials: MK
authors.lastname: Bonner
authors.forename: Daniel S
authors.initials: DS
authors.lastname: Poole
authors.forename: Tao
authors.initials: T
authors.lastname: Xu
authors.forename: Ali
authors.initials: A
authors.lastname: Sarkeshik
authors.forename: John R
authors.initials: JR
authors.lastname: Yates
authors.forename: Ahna R
authors.initials: AR
authors.lastname: Skop
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D016466:CHO Cells; D002455:Cell Division; D002462:Cell Membrane / Q000235:genetics / Q000378:metabolism; D006224:Cricetinae; D003412:Cricetulus; D006367:HeLa Cells; D006801:Humans; D008870:Microtubules / Q000235:genetics / Q000378:metabolism; D021381:Protein Transport; D020543:Proteome / Q000235:genetics / Q000378:metabolism; D040901:Proteomics; D008941:Spindle Apparatus / Q000235:genetics / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
chemical_list: D000199:Actins; D020543:Proteome