title: An advanced dual labeled gold nanoparticles probe to detect Cryptosporidium parvum using rapid immuno-dot blot assay.
abstract: The zoonotic protozoan parasite Cryptosporidium parvum poses a significant risk to public health. Due to the low infectious dose of C. parvum, remarkably sensitive detection methods are required for water and food industries analysis. However PCR affirmed sensing method of the causative nucleic acid has numerous advantages, still criterion demands for simple techniques and expertise understanding to extinguish its routine use. In contrast, protein based immuno detecting techniques are simpler to perform by a commoner, but lack of sensitivity due to inadequate signal amplification. In this paper, we focused on the development of a mere sensitive immuno detection method by coupling anti-cyst antibody and alkaline phosphatase on gold nanoparticle for C. parvum is described. Outcome of the sensitivity in an immuno-dot blot assay detection is enhanced by 500 fold (using conventional method) and visually be able to detect up to 10 oocysts/mL with minimal processing period. Techniques reported in this paper substantiate the convenience of immuno-dot blot assay for the routine screening of C. parvum in water/environmental examines and most importantly, demonstrates the potential of a prototype development of simple and inexpensive diagnostic technique.
authors.affiliation: Nanocomposites Research Group, Department of Chemical Engineering, Anna University, Chennai, TN 600025, India.
authors.forename: Chinnasamy
authors.initials: C
authors.lastname: Thiruppathiraja
authors.forename: Senthilkumar
authors.initials: S
authors.lastname: Kamatchiammal
authors.forename: Periyakaruppan
authors.initials: P
authors.lastname: Adaikkappan
authors.forename: Muthukaruppan
authors.initials: M
authors.lastname: Alagar
mesh_terms: D000469:Alkaline Phosphatase; D000818:Animals; D055910:Antibodies, Immobilized; D000913:Antibodies, Protozoan; D001483:Base Sequence; D016785:Cryptosporidium parvum / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification* / Q000472:pathogenicity; D016054:DNA, Protozoan / Q000235:genetics; D006046:Gold; D006801:Humans; D015151:Immunoblotting / Q000379:methods* / Q000706:statistics & numerical data; D057230:Limit of Detection; D053768:Metal Nanoparticles; D033661:Oocysts / Q000276:immunology; D014867:Water / Q000469:parasitology
chemical_list: D055910:Antibodies, Immobilized; D000913:Antibodies, Protozoan; D016054:DNA, Protozoan; D014867:Water; D006046:Gold; D000469:Alkaline Phosphatase