title: Role of peroxidases in the compensation of cytosolic ascorbate peroxidase knockdown in rice plants under abiotic stress.
abstract: Current studies, particularly in Arabidopsis, have demonstrated that mutants deficient in cytosolic ascorbate peroxidases (APXs) are susceptible to the oxidative damage induced by abiotic stress. In contrast, we demonstrate here that rice mutants double silenced for cytosolic APXs (APx1/2s) up-regulated other peroxidases, making the mutants able to cope with abiotic stress, such as salt, heat, high light and methyl viologen, similar to non-transformed (NT) plants. The APx1/2s mutants exhibited an altered redox homeostasis, as indicated by increased levels of H₂O₂ and ascorbate and glutathione redox states. Both mutant and NT plants exhibited similar photosynthesis (CO₂) assimilation and photochemical efficiency) under both normal and stress conditions. Overall, the antioxidative compensatory mechanism displayed by the mutants was associated with increased expression of OsGpx genes, which resulted in higher glutathione peroxidase (GPX) activity in the cytosolic and chloroplastic fractions. The transcript levels of OsCatA and OsCatB and the activities of catalase (CAT) and guaiacol peroxidase (GPOD; type III peroxidases) were also up-regulated. None of the six studied isoforms of OsApx were up-regulated under normal growth conditions. Therefore, the deficiency in cytosolic APXs was effectively compensated for by up-regulation of other peroxidases. We propose that signalling mechanisms triggered in rice mutants could be distinct from those proposed for Arabidopsis.
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Ceará, Brasil.
authors.forename: Aurenivia
authors.initials: A
authors.lastname: Bonifacio
authors.forename: Marcio O
authors.initials: MO
authors.lastname: Martins
authors.forename: Carolina W
authors.initials: CW
authors.lastname: Ribeiro
authors.forename: Adilton V
authors.initials: AV
authors.lastname: Fontenele
authors.forename: Fabricio E L
authors.initials: FE
authors.lastname: Carvalho
authors.forename: Márcia
authors.initials: M
authors.lastname: Margis-Pinheiro
authors.forename: Joaquim A G
authors.initials: JA
authors.lastname: Silveira
mesh_terms: D060387:Ascorbate Peroxidases / Q000235:genetics / Q000378:metabolism*; D002245:Carbon Dioxide / Q000378:metabolism; D002374:Catalase / Q000235:genetics / Q000378:metabolism*; D002734:Chlorophyll / Q000378:metabolism; D002736:Chloroplasts / Q000378:metabolism; D003600:Cytosol / Q000201:enzymology / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D005978:Glutathione / Q000032:analysis; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism*; D006706:Homeostasis; D006861:Hydrogen Peroxide / Q000032:analysis; D015227:Lipid Peroxidation; D012275:Oryza / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010641:Phenotype; D010788:Photosynthesis; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D017384:Sequence Deletion; D015398:Signal Transduction; D013312:Stress, Physiological; D015854:Up-Regulation
chemical_list: D010940:Plant Proteins; D002734:Chlorophyll; D002245:Carbon Dioxide; D006861:Hydrogen Peroxide; D060387:Ascorbate Peroxidases; D002374:Catalase; D005979:Glutathione Peroxidase; D005978:Glutathione