title: [Enhanced bioremediation of coking plant soils contaminated with polycyclic aromatic hydrocarbons].
abstract: Soil samples contaminated with polycyclic aromatic hydrocarbons (PAHs) were collected from Beijing Coking Plant. The purposes were to isolate PAHs degrading bacteria from the soils, determine their appropriate living condition, enrich them and apply them in the enhanced bioremediation of the contaminated soils. Using each of the 16 USEPA priority PAHs as the sole carbon source, PAHs degrading bacteria were isolated using the method of plate streaking and identified by genetic analysis. In total seven species of PAHs degrading bacteria were obtained. When mixed, these bacteria could degrade the 16 (2-6 cyclic) PAHs studied at appropriate concentrations. In the liquid medium, when the total concentration of the 16 PAHs (sigma PAH16) was 17 microg/mL, single bacteria could grow well and degrade the PAHs. However, when sigma PAH16 was 166 microg/mL, the growth and activity of either single PAHs degrading bacteria or a mixture of the seven PAHs degrading bacteria were inhibited. Aiming at the contaminated soils from Beijing coking plant, five treatments were performed, i.e., control (C), addition of nutrient (N), addition of nutrient and PAHs degrading bacteria (N + B), addition of nutrient and surfactant (N +S), addition of nutrient and PAHs degrading bacteria and surfactant (N + B + S). After five weeks of experiment, compared to the C treatment, the mean removal rate of the 16 PAHs in the N + B treatment was increased 32%, and the mean removal rate of the 16 PAHs in the N + B + S treatment was increased 46% (the mean removal rate of the 10 4-6 cyclic PAHs was increased 52%). The addition of PAHs degrading bacteria and surfactant could significantly enhance the degradation of PAHs in the soils. This study provides evidence for the enhanced bioremediation of PAHs contaminated soil for Beijing coking plant and other coking plants.
authors.affiliation: Laboratory for Earth Surface Processes, College of Urban and Environmental Sciences, Peking University, Beijing 100871, China. luxx@urban.pku.edu.cn
authors.forename: Xiao-Xia
authors.initials: XX
authors.lastname: Lu
authors.forename: Xiu-Li
authors.initials: XL
authors.lastname: Li
authors.forename: Jie
authors.initials: J
authors.lastname: Ma
authors.forename: Shu-Ke
authors.initials: SK
authors.lastname: Wu
authors.forename: Chao-Qi
authors.initials: CQ
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
mesh_terms: D001673:Biodegradation, Environmental; D003077:Coke; D052918:Environmental Restoration and Remediation / Q000379:methods*; D007220:Industrial Waste / Q000517:prevention & control; D011084:Polycyclic Aromatic Hydrocarbons / Q000737:chemistry / Q000302:isolation & purification*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D003077:Coke; D007220:Industrial Waste; D011084:Polycyclic Aromatic Hydrocarbons; D012989:Soil Pollutants