title: [The influence of SiO2 on epithelial-mesenchymal transition (EMT) in human bronchial epithelial cells].
abstract: OBJECTIVE
  To investigate SiO2-induced EMT in human bronchial epithelial cells HBE in vitro.
  
  
  METHODS
  HBE cells were cultured and then stimulated with indicated doses of SiO2 (0, 50, 100, 200, 300 µg/ml). The morphological changes were observed by microscope. In addition, Western blot was per-formed to detect the expression of E-cad, α-SMA and Vim. The changes of migration ability were examined by wound-healing assay in vitro.
  
  
  RESULTS
  (1) After exposure to SiO2, HBE cells lost contact with their neighbor and displayed a spindle-shape, fibroblast-like morphology. (2) Compared with the control, the E-cad (300 µg/ml group) expression downregulated 2.98 fold (P < 0.05), and the Vim (300 µg/ml group) and α-SMA (200 µg/ml group) expression upregulated 4.46 fold and 3.55 fold (P < 0.05). There were significant differences between 100, 200, 300 µg/ml groups and the control group (P < 0.05). (3) In the test group, the percentage of wound-healing areas/wound areas were larger than those in control group (P < 0.05).
  
  
  CONCLUSIONS
  SiO2 could induce EMT in human bronchial epithelial cells.
authors.affiliation: Department of pathology, Central South University, Changsha 410013, China.
authors.forename: Guan-nan
authors.initials: GN
authors.lastname: Liang
authors.forename: Jian-hua
authors.initials: JH
authors.lastname: Zhou
authors.forename: Yong-bin
authors.initials: YB
authors.lastname: Hu
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
authors.forename: Zhen-qin
authors.initials: ZQ
authors.lastname: Gao
authors.forename: Hai-ying
authors.initials: HY
authors.lastname: Jiang
mesh_terms: D001980:Bronchi / Q000166:cytology; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology* / Q000187:drug effects; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects*; D006801:Humans; D012822:Silicon Dioxide / Q000009:adverse effects*; D017154:Stromal Cells / Q000166:cytology* / Q000187:drug effects
chemical_list: D012822:Silicon Dioxide