title: Kanagawa-negative, tdh- and trh-positive Vibrio parahaemolyticus isolated from fresh oysters marketed in Fortaleza, Brazil.
abstract: Between October 2008 and June 2009, 15 samples of 10 live oysters each (Crassostrea rhizophorae) measuring 8.31-10.71 cm were purchased from a restaurant on the seashore of Fortaleza, Brazil. The Vibrio count ranged from 75 (estimated) to 43,500 CFU/g. Fourteen species were identified among the 56 isolated Vibrio strains, with V. parahaemolyticus as the most prevalent. Two of the 17 V. parahaemolyticus strains were urease-positive and tdh- and trh-positive on multiplex PCR, but neither produced β-hemolysis halos in Wagatsuma agar. Thus, fresh oysters served in natura in Fortaleza, Brazil, were found to contain Vibrio strains known to cause gastroenteritis in humans.
authors.affiliation: Marine Sciences Institute (LABOMAR/UFC), Fortaleza-Ceará, Brazil. reginevieira@terra.com.br
authors.forename: Regine H S F
authors.initials: RH
authors.lastname: Vieira
authors.forename: Renata A
authors.initials: RA
authors.lastname: Costa
authors.forename: Francisca G R
authors.initials: FG
authors.lastname: Menezes
authors.forename: Giselle C
authors.initials: GC
authors.lastname: Silva
authors.forename: Grace N D
authors.initials: GN
authors.lastname: Theophilo
authors.forename: Dália P
authors.initials: DP
authors.lastname: Rodrigues
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Maggioni
mesh_terms: D000818:Animals; D058491:Bacterial Load; D001426:Bacterial Proteins / Q000235:genetics*; D001938:Brazil; D003470:Culture Media / Q000737:chemistry; D005516:Food Microbiology; D006460:Hemolysin Proteins / Q000378:metabolism; D006461:Hemolysis; D010125:Ostreidae / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D014733:Vibrio / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D006460:Hemolysin Proteins; D037521:Virulence Factors