title: Different effects of intermittent and continuous fluid shear stresses on osteogenic differentiation of human mesenchymal stem cells.
abstract: A reasonable mechanical microenvironment similar to the bone microenvironment in vivo is critical to the formation of engineering bone tissues. As fluid shear stress (FSS) produced by perfusion culture system can lead to the osteogenic differentiation of human mesenchymal stem cells (hMSCs), it is widely used in studies of bone tissue engineering. However, effects of FSS on the differentiation of hMSCs largely depend on the FSS application manner. It is interesting how different FSS application manners influence the differentiation of hMSCs. In this study, we examined the effects of intermittent FSS and continuous FSS on the osteogenic differentiation of hMSCs. The phosphorylation level of ERK1/2 and FAK is measured to investigate the effects of different FSS application manners on the activation of signaling molecules. The results showed that intermittent FSS could promote the osteogenic differentiation of hMSCs. The expression level of osteogenic genes and the alkaline phosphatase (ALP) activity in cells under intermittent FSS application were significantly higher than those in cells under continuous FSS application. Moreover, intermittent FSS up-regulated the activity of ERK1/2 and FAK. Our study demonstrated that intermittent FSS is more effective to induce the osteogenic differentiation of hMSCs than continuous FSS.
authors.affiliation: Institute of Cell Biology, College of Life Sciences, Zhejiang University, Hangzhou, 310058, People's Republic of China.
authors.forename: Liyue
authors.initials: L
authors.lastname: Liu
authors.forename: Bin
authors.initials: B
authors.lastname: Yu
authors.forename: Jiarong
authors.initials: J
authors.lastname: Chen
authors.forename: Zihua
authors.initials: Z
authors.lastname: Tang
authors.forename: Chen
authors.initials: C
authors.lastname: Zong
authors.forename: Dan
authors.initials: D
authors.lastname: Shen
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zheng
authors.forename: Xiangming
authors.initials: X
authors.lastname: Tong
authors.forename: Changyou
authors.initials: C
authors.lastname: Gao
authors.forename: Jinfu
authors.initials: J
authors.lastname: Wang
mesh_terms: D000328:Adult; D000469:Alkaline Phosphatase / Q000378:metabolism; D001842:Bone and Bones / Q000166:cytology; D002454:Cell Differentiation; D049109:Cell Proliferation; D002470:Cell Survival; D006801:Humans; D020935:MAP Kinase Signaling System; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010012:Osteogenesis; D010477:Perfusion; D010766:Phosphorylation; D033081:Shear Strength; D015398:Signal Transduction; D013314:Stress, Mechanical; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D000469:Alkaline Phosphatase