title: Comparative genomic analysis of the hexuronate metabolism genes and their regulation in gammaproteobacteria.
abstract: The hexuronate metabolism in Escherichia coli is regulated by two related transcription factors from the FadR subfamily of the GntR family, UxuR and ExuR. UxuR controls the d-glucuronate metabolism, while ExuR represses genes involved in the metabolism of all hexuronates. We use a comparative genomics approach to reconstruct the hexuronate metabolic pathways and transcriptional regulons in gammaproteobacteria. We demonstrate differences in the binding motifs of UxuR and ExuR, identify new candidate members of the UxuR/ExuR regulons, and describe the links between the UxuR/ExuR regulons and the adjacent regulons UidR, KdgR, and YjjM. We provide experimental evidence that two predicted members of the UxuR regulon, yjjM and yjjN, are the subject of complex regulation by this transcription factor in E. coli.
authors.affiliation: State Research Institute of Genetics and Selection of Industrial Microorganisms (GosNIIGenetika), Moscow, Russia.
authors.forename: Inna A
authors.initials: IA
authors.lastname: Suvorova
authors.forename: Maria N
authors.initials: MN
authors.lastname: Tutukina
authors.forename: Dmitry A
authors.initials: DA
authors.lastname: Ravcheev
authors.forename: Dmitry A
authors.initials: DA
authors.lastname: Rodionov
authors.forename: Olga N
authors.initials: ON
authors.lastname: Ozoline
authors.forename: Mikhail S
authors.initials: MS
authors.lastname: Gelfand
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D053898:Biosynthetic Pathways; D020563:Gammaproteobacteria / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D023281:Genomics; D006603:Hexuronic Acids / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D018085:Regulon; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D006603:Hexuronic Acids; D014157:Transcription Factors