title: 3D vision of human lysozyme adsorbed onto a SBA-15 nanostructured matrix.
abstract: TEM images of human lysozyme loaded on SBA-15 mesoporous silica were obtained through the immunogold staining (IGS) method. IGS is based on the specific interaction between proteins and colloidal gold-conjugated antibodies. Clear evidence that protein molecules are adsorbed both on the external and on the inner pore surface is presented.
authors.affiliation: Dipartimento di Citomorfologia, Università di Cagliari, Cittadella Universitaria, S.S. 554 bivio Sestu, 09042-Monserrato (CA), Italy.
authors.forename: Monica
authors.initials: M
authors.lastname: Piras
authors.forename: Andrea
authors.initials: A
authors.lastname: Salis
authors.forename: Marco
authors.initials: M
authors.lastname: Piludu
authors.forename: Daniela
authors.initials: D
authors.lastname: Steri
authors.forename: Maura
authors.initials: M
authors.lastname: Monduzzi
mesh_terms: D000327:Adsorption; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods*; D007150:Immunohistochemistry; D046529:Microscopy, Electron, Transmission; D009113:Muramidase / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: C509969:SBA-15; D012822:Silicon Dioxide; D009113:Muramidase