title: Oxidative stress, cytokine/chemokine and disruption of blood-brain barrier in neonate rats after meningitis by Streptococcus agalactiae.
abstract: We verify the levels of cytokine/chemokine, myeloperoxidase activity, oxidative stress and disruption of BBB in hippocampus and cortex of the neonate Wistar rats after meningitis by S. agalactiae. In the hippocampus the levels were increased of CINC-1 at 6 h and 12 h, IL-1β at 6, 12 and 24 h, IL-6 at 6, 24 and 96 h, IL-10 at 24, 48 and 96 h and TNF-α at 24 h and 96 h. In the cortex the CINC-1 and IL-1β levels were found increased at 6 h. The MPO activity was significantly elevated at 24, 48 and 98 h in hippocampus and at 6, 12, 24, 48 and 96 h in the cortex. The breakdown of BBB started at 12 h.TBARS levels were elevated in the hippocampus at 6, 12, 24, 48, 72 and 96 h and cortex at 72 and 96 h. Protein carbonyls were elevated in the hippocampus and cortex at 6, 24, 48, 72 and 96 h. There was a decrease of SOD activity in hippocampus and in cortex. Catalase activity was elevated in hippocampus at 6 h and in the cortex at 12 and 96 h. Neonatal bacterial infections of the CNS are severe, the interference with the complex network of cytokines/chemokine, other inflammatory mediators and oxidants tend to aggravate the illness and can be involved in the breakdown of the BBB.
authors.affiliation: Laboratório de Microbiologia Experimental and Instituto Nacional de Ciência e Tecnologia Translacional em Medicina, Unidade Acadêmica de Ciências da Saúde, Universidade do Extremo Sul Catarinense, Criciúma, SC, Brazil. tba@unesc.net
authors.forename: Tatiana
authors.initials: T
authors.lastname: Barichello
authors.forename: Joelson C
authors.initials: JC
authors.lastname: Lemos
authors.forename: Jaqueline S
authors.initials: JS
authors.lastname: Generoso
authors.forename: Andreza L
authors.initials: AL
authors.lastname: Cipriano
authors.forename: Graziele L
authors.initials: GL
authors.lastname: Milioli
authors.forename: Danielle M
authors.initials: DM
authors.lastname: Marcelino
authors.forename: Francieli
authors.initials: F
authors.lastname: Vuolo
authors.forename: Fabricia
authors.initials: F
authors.lastname: Petronilho
authors.forename: Felipe
authors.initials: F
authors.lastname: Dal-Pizzol
authors.forename: Márcia Carvalho
authors.initials: MC
authors.lastname: Vilela
authors.forename: Antonio Lucio
authors.initials: AL
authors.lastname: Teixeira
mesh_terms: D000328:Adult; D000818:Animals; D000831:Animals, Newborn; D001812:Blood-Brain Barrier / Q000378:metabolism* / Q000473:pathology; D002374:Catalase / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism; D018925:Chemokines / Q000378:metabolism*; D016207:Cytokines / Q000378:metabolism*; D005260:Female; D006624:Hippocampus / Q000378:metabolism; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D053208:Kaplan-Meier Estimate; D008297:Male; D016920:Meningitis, Bacterial / Q000382:microbiology / Q000473:pathology / Q000503:physiopathology*; D018384:Oxidative Stress; D009195:Peroxidase / Q000378:metabolism; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D013290:Streptococcal Infections / Q000382:microbiology / Q000473:pathology / Q000503:physiopathology*; D013292:Streptococcus agalactiae / Q000472:pathogenicity*; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D018925:Chemokines; D016207:Cytokines; D017392:Thiobarbituric Acid Reactive Substances; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase