title: Hyperbaric oxygenation promotes regeneration of biliary cells and improves cholestasis in rats.
abstract: OBJECTIVE
  To investigate the effects of hyperbaric oxygenation (HBO) on regeneration of the biliary ductal system and postoperative cholestasis in hepatectomized rats.
  
  
  METHODS
  HBO was performed in Wistar rats daily starting 12 h after a 70% partial hepatectomy. Regenerated liver weight, serum parameters and the proliferating cell nuclear antigen labeling index of hepatocytes and biliary ductal cells were measured. Hepatocyte growth factor (HGF), c-Met and transforming growth factor (TGF) β-1 mRNA expression levels were analyzed by quantitative reverse transcription polymerase chain reaction.
  
  
  RESULTS
  HBO improved the postoperative serum levels of total bile acid but not transaminase levels. HBO promoted hepatocyte and biliary ductal cell proliferation. The hematoxylin and eosin-stained specimens revealed fewer ballooned hepatocytes and higher cell densities in the HBO group compared to the control group. HBO suppressed c-Met mRNA levels at 15 h but did not modulate HGF or TGF β-1 mRNA expression levels.
  
  
  CONCLUSIONS
  HBO promoted regeneration of biliary ductal cells and improved postoperative cholestasis after a partial hepatectomy.
authors.affiliation: Department of General Surgical Science, Gunma University, Graduate School of Medicine, 3-39-22 Showamachi, Maebashi, Gunma 371-8511, Japan. a_idetsu@yahoo.co.jp
authors.forename: Akihito
authors.initials: A
authors.lastname: Idetsu
authors.forename: Taketoshi
authors.initials: T
authors.lastname: Suehiro
authors.forename: Kohji
authors.initials: K
authors.lastname: Okada
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Shimura
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kuwano
mesh_terms: D000818:Animals; D001647:Bile Acids and Salts / Q000097:blood; D001652:Bile Ducts / Q000166:cytology*; D002779:Cholestasis / Q000628:therapy*; D006498:Hepatectomy; D017228:Hepatocyte Growth Factor / Q000235:genetics; D006931:Hyperbaric Oxygenation; D008115:Liver Regeneration; D008297:Male; D019859:Proto-Oncogene Proteins c-met / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D053773:Transforming Growth Factor beta1 / Q000235:genetics
chemical_list: D001647:Bile Acids and Salts; D053773:Transforming Growth Factor beta1; D017228:Hepatocyte Growth Factor; D019859:Proto-Oncogene Proteins c-met