title: Molecular recognition force spectroscopy of a specific lectin-carbohydrate interaction at single-molecule level.
abstract: Carbohydrates are involved in many essential biological recognition processes in physiological and pathological states. Thus, it is important to understand the mechanism of protein-carbohydrate interactions at molecular level. In the present study, molecular recognition force spectroscopy was applied to investigate the interactions between RCA₁₂₀, a lectin from Ricinus communis, and galactose (Gal) and asialofetuin (ASF) at the single-molecule level. RCA₁₂₀ coupled to the AFM tip could specifically recognize Gal and ASF, respectively. The unbinding forces of RCA₁₂₀-Gal and RCA₁₂₀-ASF increase linearly with the logarithm of loading rate. The results reveal that the binding capability of RCA₁₂₀ toward Gal is weaker than that of ASF, implicating a multivalent effect in the RCA₁₂₀-ASF interaction.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, People's Republic of China.
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Li
authors.forename: Haiyan
authors.initials: H
authors.lastname: Qiao
authors.forename: Chunyan
authors.initials: C
authors.lastname: Xing
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Lai-Xi
authors.initials: LX
authors.lastname: Wang
authors.forename: Hongda
authors.initials: H
authors.lastname: Wang
authors.forename: Bailin
authors.initials: B
authors.lastname: Zhang
authors.forename: Jilin
authors.initials: J
authors.lastname: Tang
mesh_terms: D000465:Algorithms; D001212:Asialoglycoproteins / Q000737:chemistry*; D060748:Fetuins / Q000737:chemistry*; D005690:Galactose / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D037121:Plant Lectins / Q000737:chemistry*; D011485:Protein Binding; D012825:Silicon / Q000737:chemistry; D013499:Surface Properties; D013816:Thermodynamics
chemical_list: D001212:Asialoglycoproteins; D060748:Fetuins; D037121:Plant Lectins; C017707:Ricinus communis agglutinin-1; C017029:asialofetuin; D005690:Galactose; D012825:Silicon