title: [Methylation profiling of the cell cycle regulating genes in placenta of human embryos with chromosomal mosaicism].
abstract: To date the investigation of epigenetic mechanisms of cell cycle regulation, ensuring genomic stability maintaining and accurate transfer of hereditary information to daughter cells, is of a considerable interest. Development of the up-to-date molecular technologies allows determining methylation pattern of the whole genome at a single round of analysis. In this work for the first time the epigenetic status of placental tissues of human embryos with mosaic karyotype was studied using the genome-wide Illumina Infinium HumanMethylation27 BeadChip Array (Illumina, USA). The groups of genes, related to the cell cycle and its regulation and containing differentially methylated CpG-sites in their promoter regions, are determined. The methylation level of oncogenes (ARHGEF1, RGF5), tumor-suppressors (APC2, BRACA2, DCC, GRLF1, RB1, TP73, TSPYL2, VHL) as well as genes, participating in chromosome segregation regulation (CNTROB, GMNN, PROCR, TACC1), was changed most frequently.
authors.forename: A A
authors.initials: AA
authors.lastname: Kashevarova
authors.forename: E N
authors.initials: EN
authors.lastname: Tolmacheva
authors.forename: E A
authors.initials: EA
authors.lastname: Sazhenova
authors.forename: N N
authors.initials: NN
authors.lastname: Sikhanova
authors.forename: I N
authors.initials: IN
authors.lastname: Lebedev
mesh_terms: D002453:Cell Cycle / Q000235:genetics*; D018797:Cell Cycle Proteins / Q000235:genetics; D018899:CpG Islands / Q000235:genetics; D019175:DNA Methylation; D057890:Epigenomics; D005260:Female; D016147:Genes, Tumor Suppressor; D006801:Humans; D046228:Microarray Analysis; D009030:Mosaicism / Q000196:embryology*; D009857:Oncogenes / Q000235:genetics; D010920:Placenta / Q000166:cytology* / Q000378:metabolism; D011247:Pregnancy
chemical_list: D018797:Cell Cycle Proteins