title: High-throughput screening of small molecule libraries using SAMDI mass spectrometry.
abstract: High-throughput screening is a common strategy used to identify compounds that modulate biochemical activities, but many approaches depend on cumbersome fluorescent reporters or antibodies and often produce false-positive hits. The development of "label-free" assays addresses many of these limitations, but current approaches still lack the throughput needed for applications in drug discovery. This paper describes a high-throughput, label-free assay that combines self-assembled monolayers with mass spectrometry, in a technique called SAMDI, as a tool for screening libraries of 100,000 compounds in one day. This method is fast, has high discrimination, and is amenable to a broad range of chemical and biological applications.
authors.forename: Zachary A
authors.initials: ZA
authors.lastname: Gurard-Levin
authors.forename: Michael D
authors.initials: MD
authors.lastname: Scholle
authors.forename: Adam H
authors.initials: AH
authors.lastname: Eisenberg
authors.forename: Milan
authors.initials: M
authors.lastname: Mrksich
mesh_terms: D001331:Automation; D020650:Combinatorial Chemistry Techniques / Q000295:instrumentation; D057166:High-Throughput Screening Assays / Q000295:instrumentation* / Q000379:methods*; D056572:Histone Deacetylase Inhibitors / Q000819:agonists / Q000302:isolation & purification; D006655:Histone Deacetylases / Q000737:chemistry; D015394:Molecular Structure; D054852:Small Molecule Libraries / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013678:Technology, Pharmaceutical
chemical_list: D056572:Histone Deacetylase Inhibitors; D054852:Small Molecule Libraries; D006655:Histone Deacetylases