title: Role of glutathione-S-transferase P1 hypermethylation in molecular detection of prostate cancer.
abstract: OBJECTIVE
  The aim of this study was to evaluate the diagnostic potential of the quantification of glutathione-S-transferase P1 (GSTP1) gene hypermethylation in molecular detection of prostate cancer in tissue biopsies.
  
  
  METHODS
  One hundred fourteen male patients were enrolled in the study; 44 patients with histopathologically confirmed prostate adenocarcinoma, 20 patients with variable degrees of prostate intraepithelial neoplasia, and 50 with benign prostatic hyperplasia, who served as a control group. Real-time quantitative methylation-specific polymerase chain reaction was used for assessment of methylation of the promoter region of the GSTP1 gene.
  
  
  RESULTS
  Methylation of the GSTP1 promotor was detected in 24% of patients with benign prostatic hyperplasia, 60% of patients with prostate intraepithelial neoplasia, and in 86.3% of prostate adenocarcinoma patients. A statistically significant difference in the GSTP1/MYOD1 (myogenic differentiation 1gene) methylation ratios among the three groups was observed (p=0.0001). At the cutoff value of 9, GSTP1/MYOD1 methylation ratios showed sensitivity in the detection of prostate adenocarcinoma of 71.8% and specificity of 96%.
  
  
  CONCLUSIONS
  Methylation of the GSTP1 promotor is a common molecular alteration in prostate cancer that may be a useful adjunct to serum screening tests and digital rectal examination findings and the use of quantitative real-time methylation-specific polymerase chain reaction is a promising technique that often distinguishes malignant from nonmalignant prostate disease.
authors.affiliation: Department of Clinical Pathology, Alexandria University, Alexandria, Egypt. doaa_hashad2003@yahoo.com
authors.forename: Doaa I
authors.initials: DI
authors.lastname: Hashad
authors.forename: Mohamed Mohie Eldin I
authors.initials: MM
authors.lastname: Hashad
authors.forename: Iman M
authors.initials: IM
authors.lastname: Talaat
authors.forename: Mona A
authors.initials: MA
authors.lastname: Ibrahim
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D016022:Case-Control Studies; D019175:DNA Methylation / Q000502:physiology*; D003937:Diagnosis, Differential; D051549:Glutathione S-Transferase pi / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D006801:Humans; D008297:Male; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009367:Neoplasm Staging; D011237:Predictive Value of Tests; D011470:Prostatic Hyperplasia / Q000175:diagnosis / Q000235:genetics / Q000473:pathology; D019048:Prostatic Intraepithelial Neoplasia / Q000175:diagnosis / Q000235:genetics / Q000473:pathology; D011471:Prostatic Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D012680:Sensitivity and Specificity
chemical_list: D051549:Glutathione S-Transferase pi