title: Reprogramming of mouse and human cells to pluripotency using mature microRNAs.
abstract: Induced pluripotent stem cells (iPSCs) can be generated from differentiated human and mouse somatic cells using transcription factors such as Oct4, Sox2, Klf4, and c-Myc. It is possible to augment the reprogramming process with chemical compounds, but issues related to low reprogramming efficiencies and, with a number of protocols, residual vector sequences, remain to be resolved. We show here that it is possible to reprogram mouse and human cells to pluripotency by direct transfection of mature double-stranded microRNAs (miRNAs). Our approaches use a combination of mir-200c plus mir-302 s and mir-369 s family miRNAs. Because this reprogramming method does not require vector-based gene transfer, it holds significant potential for biomedical research and regenerative medicine.
authors.affiliation: Department of Gastroenterological Surgery, Osaka University Graduate School of Medicine, Suita, Yamadaoka 2-2, Osaka 565-0871, Japan.
authors.forename: Norikatsu
authors.initials: N
authors.lastname: Miyoshi
authors.forename: Hideshi
authors.initials: H
authors.lastname: Ishii
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Nagano
authors.forename: Naotsugu
authors.initials: N
authors.lastname: Haraguchi
authors.forename: Dyah Laksmi
authors.initials: DL
authors.lastname: Dewi
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Kano
authors.forename: Shinpei
authors.initials: S
authors.lastname: Nishikawa
authors.forename: Masahiro
authors.initials: M
authors.lastname: Tanemura
authors.forename: Koshi
authors.initials: K
authors.lastname: Mimori
authors.forename: Fumiaki
authors.initials: F
authors.lastname: Tanaka
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Saito
authors.forename: Junichi
authors.initials: J
authors.lastname: Nishimura
authors.forename: Ichiro
authors.initials: I
authors.lastname: Takemasa
authors.forename: Tsunekazu
authors.initials: T
authors.lastname: Mizushima
authors.forename: Masataka
authors.initials: M
authors.lastname: Ikeda
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Yamamoto
authors.forename: Mitsugu
authors.initials: M
authors.lastname: Sekimoto
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Doki
authors.forename: Masaki
authors.initials: M
authors.lastname: Mori
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D065150:Cellular Reprogramming; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism*; D000090062:Kruppel-Like Factor 4; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D044968:Regenerative Medicine / Q000379:methods; D014157:Transcription Factors / Q000235:genetics; D014162:Transfection
chemical_list: C000715467:KLF4 protein, human; C000715468:Klf4 protein, mouse; D000090062:Kruppel-Like Factor 4; D035683:MicroRNAs; D014157:Transcription Factors