title: Molecular typing of Brucella species isolates from livestock and human.
abstract: Although host specificity has been observed in different species of Brucella, crossing the animal host boundary is likely to occur at any time. In this study, Bruce ladder PCR and abortus-melitensis-ovis-suis (AMOS) PCR assays were used to characterize 47 Brucella isolates from Indian origin in order to know exact species for understanding epidemiology of brucellosis. Out of them, 28, 14, and 5 isolates were found to be Brucella abortus, Brucella melitensis, and Brucella suis, respectively. Further analysis by AMOS PCR has identified that all the B. abortus isolates belong to any one of the biovar 1, 2, or 4; of the five B. suis isolates, three belong to biovar 1 and two belong to any one of the biovar 2, 3, 4, or 5. Although this multiplex Bruce ladder PCR is useful in differentiating all Brucella species, elaborate study is required to further characterize the isolates at exact biovar level.
authors.affiliation: Project Directorate on Animal Disease Monitoring and Surveillance (PD_ADMAS), Hebbal, Bangalore, 560 024, India. nagar75@gmail.com
authors.forename: Mohandoss
authors.initials: M
authors.lastname: Nagalingam
authors.forename: Rajeswari
authors.initials: R
authors.lastname: Shome
authors.forename: Vinayagamurthy
authors.initials: V
authors.lastname: Balamurugan
authors.forename: Bibek Ranjan
authors.initials: BR
authors.lastname: Shome
authors.forename: Krishnamsetty
authors.initials: K
authors.lastname: NarayanaRao
authors.lastname: Vivekananda
authors.forename: Shrikrishna
authors.initials: S
authors.lastname: Isloor
authors.forename: Krishnamsetty
authors.initials: K
authors.lastname: Prabhudas
mesh_terms: D000818:Animals; D002002:Brucella / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D002006:Brucellosis / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology* / Q000662:veterinary*; D002007:Brucellosis, Bovine / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology; D002020:Buffaloes; D002417:Cattle; D004269:DNA, Bacterial / Q000032:analysis; D006801:Humans; D007194:India / Q000453:epidemiology; D058751:Livestock; D058889:Molecular Typing / Q000379:methods*; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods* / Q000662:veterinary; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial