title: Significant association of antiphospholipid antibodies and TNF-alpha: marker of severe atherogenic profile of patients with type II diabetes mellitus without micro and/or macrovascular complications.
abstract: OBJECTIVE
  In vitro, stimulation of monocytes with antiphospholipid (aPL) antibodies resulted in increased secretion of TNF-alpha, but association of aPL with features of diabetes mellitus is not clarified yet. Therefore, we investigated the distribution of anticardiolipin (aCL), anti-β2gpI (aβ2gpI), anti-annexin A5 (aannxA5), and anti-oxLDL (aoxLDL) antibodies, and TNF-alpha in well-formed group of 78 patients with type II diabetes mellitus without vascular complications.
  
  
  METHODS
  Investigated antibodies and TNF-alpha concentrations were measured by ELISA.
  
  
  RESULTS
  Antiphospholipid antibodies were in positive correlation with TNF-alpha concentrations: aCL IgG (r=0.303, p=0.007), aCL IgM (r=0.386, p=0.000), aβ2gpI IgG (r=0.499, p=0.000), aβ2gpI IgM (r=0.462, p=0.000), aanxA5 IgG (r=0.479, p=0.000), aanxA5 IgM (r=0.641, p=0.000), aoxLDL (IgG+IgM, r=0.279, p=0.000). Anticardiolipin-positive and aCL-negative subgroups differed in TNF-alpha concentrations (Mann-Whitney, p=0.032). Significantly elevated LDL concentrations were noticed in aCL-positive patients with disease duration 10-15 years (χ(2)=15.000, p=0.000) and apoB concentrations were elevated in aoxLDL-positive patients with disease duration 7-10 years (χ(2)=3.938, p=0.047).
  
  
  CONCLUSIONS
  Significant association of antiphospholipid antibodies and TNF-alpha might be a marker of severe atherogenic profile (suggested by increased levels of lipids in aPL-positive subgroups) and should be used for the stratification of patients with an increased risk for future deterioration of the disease.
authors.affiliation: Medical Faculty, University of Novi Sad, Hajduk Veljka 3, Serbia. mb58303@gmail.com
authors.forename: Mirjana
authors.initials: M
authors.lastname: Becarevic
authors.forename: Jelena
authors.initials: J
authors.lastname: Seferovic
authors.forename: Svetlana
authors.initials: S
authors.lastname: Ignjatovic
authors.forename: Sandra
authors.initials: S
authors.lastname: Singh
authors.forename: Nada
authors.initials: N
authors.lastname: Majkic-Singh
mesh_terms: D000328:Adult; D017152:Antibodies, Antiphospholipid / Q000276:immunology*; D015415:Biomarkers / Q000097:blood; D003924:Diabetes Mellitus, Type 2 / Q000150:complications* / Q000276:immunology*; D005260:Female; D006801:Humans; D008297:Male; D008833:Microcirculation; D008875:Middle Aged; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology*; D014652:Vascular Diseases / Q000209:etiology* / Q000276:immunology*
chemical_list: D017152:Antibodies, Antiphospholipid; D015415:Biomarkers; D014409:Tumor Necrosis Factor-alpha