title: Nutritional support in the treatment of aplastic anemia.
abstract: OBJECTIVE
  Whether a specific nutritional support promotes healing of aplastic anemia (AA) patients is still unclear. Therefore, we explored the potential of a high-nucleotide, arginine, and micronutrient nutritional supplement on the nutritional rehabilitation of AA mice.
  
  
  METHODS
  The BALB/c AA mice model was treated with hypodermic injections of acetylphenylhydrazine (100 mg/kg), x-ray (2.0 Gy), and intraperitoneal injections of a cyclophosphamide (80 mg/kg) combination. Then AA mice were fed with nutritional supplements in a dose-dependent manner (1445.55, 963.7, 674.59 mg/kg/d) for 7 wk. At the end of the experimental period, mice were autopsied. A full blood count was performed, and femoral marrow cell suspensions were prepared to assess the total femoral nucleated cell count and the number of committed hemopoietic progenitor cells (colony-forming units). The pathologic changes of liver and spleen were analyzed.
  
  
  RESULTS
  The significant increases of nutrient mixture groups were evident in many peripheral blood parameters. The femoral nucleated cell count and colony-forming units of nutritional supplements groups were markedly increased, compared with the AA group. Transmission electron microscopy showed that the number of mitochondria in similar bone marrow cells was increased in nutritional supplements groups. The nutritional supplements also affected the recovery of livers and spleens of AA mice.
  
  
  CONCLUSIONS
  Specific nutritional supplements accelerated rehabilitation of AA mice and can be used as nutritional support in the treatment of AA.
authors.affiliation: College of Laboratory Medicine, Dalian Medical University, Dalian 116044, Liaoning Province, China.
authors.forename: Li
authors.initials: L
authors.lastname: Jia
authors.forename: Jingda
authors.initials: J
authors.lastname: Yu
authors.forename: Ling
authors.initials: L
authors.lastname: He
authors.forename: Huaxin
authors.initials: H
authors.lastname: Wang
authors.forename: Lili
authors.initials: L
authors.lastname: Jiang
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Miao
authors.forename: Wenguo
authors.initials: W
authors.lastname: Wu
authors.forename: Peiman
authors.initials: P
authors.lastname: Yang
mesh_terms: D000741:Anemia, Aplastic / Q000178:diet therapy* / Q000473:pathology*; D000818:Animals; D001772:Blood Cell Count; D003520:Cyclophosphamide / Q000633:toxicity; D019587:Dietary Supplements; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004921:Erythropoietin / Q000097:blood; D006412:Hematopoietic Stem Cells / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D046529:Microscopy, Electron, Transmission; D008928:Mitochondria / Q000187:drug effects; D018529:Nutritional Support / Q000379:methods*; D010659:Phenylhydrazines / Q000633:toxicity; D012307:Risk Factors; D013154:Spleen / Q000187:drug effects / Q000473:pathology
chemical_list: D010659:Phenylhydrazines; D004921:Erythropoietin; D003520:Cyclophosphamide; C006384:N(1)-acetylphenylhydrazine