title: Peptide nucleic acid Pt(II) conjugates: a preliminary study of antisense effects in Xenopus laevis.
abstract: The avid hybridization of peptide nucleic acid (PNA) to DNA and RNA, coupled with the analogue's stability toward enzymatic degradation, has led to its investigation as an antigene/antisense agent. PNA targeted toward the 5'-UTR of an mRNA transcript can effect efficient silencing; however, if targeted to an area within the coding region, the PNA can be displaced by the moving ribosome and be an ineffective antisense agent. Platinum-appended and standard PNAs antisense to an area within the open-reading frame of the gene noggin, were injected into Xenopus laevis embryos. Phenotypic responses were observed and the preliminary results are reported herein.
authors.affiliation: Department of Chemistry, The University of Western Ontario, London, Ontario, Canada.
authors.forename: David W
authors.initials: DW
authors.lastname: Dodd
authors.forename: Sashko
authors.initials: S
authors.lastname: Damjanovski
authors.forename: Robert E
authors.initials: RE
authors.lastname: Hudson
mesh_terms: D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics; D016376:Oligonucleotides, Antisense / Q000737:chemistry* / Q000235:genetics* / Q000494:pharmacology; D016366:Open Reading Frames / Q000235:genetics; D020135:Peptide Nucleic Acids / Q000737:chemistry* / Q000235:genetics* / Q000494:pharmacology; D010984:Platinum / Q000737:chemistry*; D014982:Xenopus laevis / Q000235:genetics*; D000099176:Noggin Protein
chemical_list: D002352:Carrier Proteins; D016376:Oligonucleotides, Antisense; D020135:Peptide Nucleic Acids; D010984:Platinum; D000099176:Noggin Protein