title: The cell surface proteome of human mesenchymal stromal cells.
abstract: BACKGROUND
  Multipotent human mesenchymal stromal cells (hMSCs) are considered as promising biological tools for regenerative medicine. Their antibody-based isolation relies on the identification of reliable cell surface markers.
  
  
  RESULTS
  To obtain a comprehensive view of the cell surface proteome of bone marrow-derived hMSCs, we have developed an analytical pipeline relying on cell surface biotinylation of intact cells using cell impermeable, cleavable sulfo-NHS-SS-biotin to enrich the plasma membrane proteins and mass spectrometry for identification with extremely high confidence. Among the 888 proteins identified, we found ≈200 bona fide plasma membrane proteins including 33 cell adhesion molecules and 26 signaling receptors. In total 41 CD markers including 5 novel ones (CD97, CD112, CD239, CD276, and CD316) were identified. The CD markers are distributed homogenously within plastic-adherent hMSC populations and their expression is modulated during the process of adipogenesis or osteogenesis. Moreover, our in silico analysis revealed a significant difference between the cell surface proteome of hMSCs and that of human embryonic stem cells reported previously.
  
  
  CONCLUSIONS
  Collectively, our analytical methods not only provide a basis for further studies of mechanisms maintaining the multipotency of hMSCs within their niches and triggering their differentiation after signaling, but also a toolbox for a refined antibody-based identification of hMSC populations from different tissues and their isolation for therapeutic intervention.
authors.affiliation: Biotechnology Center, Dresden University of Technology, Dresden, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Niehage
authors.forename: Charlotte
authors.initials: C
authors.lastname: Steenblock
authors.forename: Theresia
authors.initials: T
authors.lastname: Pursche
authors.forename: Martin
authors.initials: M
authors.lastname: Bornhäuser
authors.forename: Denis
authors.initials: D
authors.lastname: Corbeil
authors.forename: Bernard
authors.initials: B
authors.lastname: Hoflack
mesh_terms: D050156:Adipogenesis; D000328:Adult; D015703:Antigens, CD / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D019070:Cell Lineage; D002462:Cell Membrane / Q000378:metabolism*; D053595:Embryonic Stem Cells / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D008565:Membrane Proteins / Q000302:isolation & purification / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D010012:Osteogenesis; D020543:Proteome / Q000302:isolation & purification / Q000378:metabolism*; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D015415:Biomarkers; D008565:Membrane Proteins; D020543:Proteome