title: High levels of genetic differentiation and selfing in the Brazilian cerrado fruit tree Dipteryx alata Vog. (Fabaceae).
abstract: Dipteryx alata is a native fruit tree species of the cerrado (Brazilian savanna) that has great economic potential because of its multiple uses. Knowledge of how the genetic variability of this species is organized within and among populations would be useful for genetic conservation and breeding programs. We used nine simple sequence repeat (SSR) primers developed for Dipteryx odorata to evaluate the genetic structure of three populations of D. alata located in central Brazil based on a leaf sample analysis from 101 adults. The outcrossing rate was evaluated using 300 open-pollinated offspring from 25 seed-trees. Pollen dispersal was measured by parentage analysis. We used spatial genetic structure (SGS) to test the minimal distance for harvesting seeds in conservation and breeding programs. Our data indicate that the populations studied had a high degree of genetic diversity and population structure, as suggested by the high level of divergence among populations . The estimated outcrossing rate suggested a mixed mating system, and the intrapopulation fixation index was influenced by SGS. We conclude that seed harvesting for genetic conservation and breeding programs requires a minimum distance between trees of 196 m to avoid collecting seeds from related seed-trees.
authors.affiliation: Laboratório de Reprodução e Genética de Espécies Arbóreas, Departamento de Ciências Florestais, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Piracicaba, SP Brazil.
authors.forename: Roberto
authors.initials: R
authors.lastname: Tarazi
authors.forename: Maria Andréia
authors.initials: MA
authors.lastname: Moreno
authors.forename: Flávio Bertin
authors.initials: FB
authors.lastname: Gandara
authors.forename: Elza Martins
authors.initials: EM
authors.lastname: Ferraz
authors.forename: Mário Luiz Teixeira
authors.initials: ML
authors.lastname: Moraes
authors.forename: Christina Cleo
authors.initials: CC
authors.lastname: Vinson
authors.forename: Ana Yamaguishi
authors.initials: AY
authors.lastname: Ciampi
authors.forename: Roland
authors.initials: R
authors.lastname: Vencovsky
authors.forename: Paulo Yoshio
authors.initials: PY
authors.lastname: Kageyama