title: Asymmetric composite membranes from chitosan and tricalcium phosphate useful for guided bone regeneration.
abstract: To fulfill the properties of barrier membranes useful for guided bone tissue regeneration in the treatment of periodontitis, in this study a simple process combining lyophilization with preheating treatment to produce asymmetric barrier membranes from biodegradable chitosan (CS) and functional β-tricalcium phosphate (TCP) was proposed. By preheating TCP/CS (3:10, w/w) in an acetic acid solution at 40°C, a skin layer that could greatly increase the mechanical properties of the membrane was formed. The asymmetric membrane with a skin layer had a modulus value almost 4-times that of the symmetric porous membrane produced only by lyophilization. This is beneficial for maintaining a secluded space for the bone regeneration, as well as to prevent the invasion of other tissues. The subsequent lyophilization at -20°C then gave the rest of material an interconnected pore structure with high porosity (83.9-90.6%) and suitable pore size (50-150 μm) which could promote the permeability and adhesiveness to bone cells, as demonstrated by the in vitro cell-culture of hFOB1.19 osteoblasts. Furthermore, the TCP particles added to CS could further increase the rigidity and the cell attachment and proliferation of hFOB1.19. The TCP/CS asymmetric composite membrane thus has the potential to be used as the barrier membrane for guided bone regeneration.
authors.affiliation: a Department of Chemical and Materials Engineering , Tamkang University , Danshui District , New Taipei City , 25137 , Taiwan.
authors.forename: Hung-Yin
authors.initials: HY
authors.lastname: Tai
authors.forename: Shiu-Huey
authors.initials: SH
authors.lastname: Chou
authors.forename: Liao-Ping
authors.initials: LP
authors.lastname: Cheng
authors.forename: Hung-Te
authors.initials: HT
authors.lastname: Yu
authors.forename: Trong-Ming
authors.initials: TM
authors.lastname: Don
mesh_terms: D019342:Acetic Acid / Q000737:chemistry; D000681:Amylases / Q000737:chemistry; D000818:Animals; D002130:Calcium Phosphates / Q000737:chemistry; D002448:Cell Adhesion; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D048271:Chitosan / Q000737:chemistry; D018920:Coculture Techniques; D005347:Fibroblasts / Q000502:physiology; D016557:Guided Tissue Regeneration, Periodontal / Q000295:instrumentation* / Q000379:methods; D006358:Hot Temperature; D006801:Humans; D008422:Materials Testing; D008567:Membranes, Artificial; D051379:Mice; D009113:Muramidase / Q000737:chemistry; D010006:Osteoblasts / Q000502:physiology; D010539:Permeability; D016062:Porosity; D014867:Water / Q000737:chemistry
chemical_list: D002130:Calcium Phosphates; D008567:Membranes, Artificial; C485817:beta-tricalcium phosphate; D014867:Water; D048271:Chitosan; D000681:Amylases; D009113:Muramidase; D019342:Acetic Acid