title: In vitro and in vivo germ line potential of stem cells derived from newborn mouse skin.
abstract: We previously reported that fetal porcine skin-derived stem cells were capable of differentiation into oocyte-like cells (OLCs). Here we report that newborn mice skin-derived stem cells are also capable of differentiating into early OLCs. Using stem cells from mice that are transgenic for Oct4 germline distal enhancer-GFP, germ cells resulting from their differentiation are expected to be GFP(+). After differentiation, some GFP(+) OLCs reached 40-45 µM and expressed oocyte markers. Flow cytometric analysis revealed that ∼ 0.3% of the freshly isolated skin cells were GFP(+). The GFP-positive cells increased to ∼ 7% after differentiation, suggesting that the GFP(+) cells could be of in vivo origin, but are more likely induced upon being cultured in vitro. To study the in vivo germ cell potential of skin-derived cells, they were aggregated with newborn ovarian cells, and transplanted under the kidney capsule of ovariectomized mice. GFP(+) oocytes were identified within a subpopulation of follicles in the resulting growth. Our finding that early oocytes can be differentiated from mice skin-derived cells in defined medium may offer a new in vitro model to study germ cell formation and oogenesis.
authors.affiliation: Department of Animal and Poultry Science, University of Guelph, Guelph, Ontario, Canada.
authors.forename: Paul W
authors.initials: PW
authors.lastname: Dyce
authors.forename: Jinghe
authors.initials: J
authors.lastname: Liu
authors.forename: Chandrakant
authors.initials: C
authors.lastname: Tayade
authors.forename: Gerald M
authors.initials: GM
authors.lastname: Kidder
authors.forename: Dean H
authors.initials: DH
authors.lastname: Betts
authors.forename: Julang
authors.initials: J
authors.lastname: Li
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D005260:Female; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry; D051379:Mice; D008822:Mice, Transgenic; D050814:Octamer Transcription Factor-3 / Q000235:genetics / Q000378:metabolism; D009866:Oogenesis / Q000235:genetics / Q000502:physiology; D010053:Ovary / Q000166:cytology / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000166:cytology / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism
chemical_list: D050814:Octamer Transcription Factor-3