title: Serine/threonine-protein phosphatase 1 α levels are paralleling olfactory memory formation in the CD1 mouse.
abstract: Although olfactory discrimination has already been studied in several mouse strains, data on protein levels linked to olfactory memory are limited. Wild mouse strains Mus musculus musculus, Mus musculus domesticus and CD1 laboratory outbred mice were tested in a conditioned odor preference task and trained to discriminate between two odors, Rose and Lemon, by pairing one odor with a sugar reward. Six hours following the final test, mice were sacrificed and olfactory bulbs (OB) were taken for gel-based proteomics analyses and immunoblotting. OB proteins were extracted, separated by 2-DE and quantified using specific software (Proteomweaver). Odor-trained mice showed a preference for the previously rewarded odor suggesting that conditioned odor preference occurred. In CD1 mice levels, one out of 482 protein spots was significantly increased in odor-trained mice as compared with the control group; it was in-gel digested by trypsin and chymotrypsin and analyzed by tandem mass spectrometry (nano-ESI-LC-MS/MS). The spot was unambiguously identified as serine/threonine-protein phosphatase PP1-α catalytic subunit (PP-1A) and differential levels observed in gel-based proteomic studies were verified by immunoblotting. PP-1A is a key signalling element in synaptic plasticity and memory processes and is herein shown to be paralleling olfactory discrimination representing olfactory memory.
authors.affiliation: Division of Neuroproteomics, Department of Pediatrics, Medical University of Vienna, Vienna, Austria.
authors.forename: Christiana
authors.initials: C
authors.lastname: Winding
authors.forename: Yanwei
authors.initials: Y
authors.lastname: Sun
authors.forename: Harald
authors.initials: H
authors.lastname: Höger
authors.forename: Hermann
authors.initials: H
authors.lastname: Bubna-Littitz
authors.forename: Arnold
authors.initials: A
authors.lastname: Pollak
authors.forename: Peter
authors.initials: P
authors.lastname: Schmidt
authors.forename: Gert
authors.initials: G
authors.lastname: Lubec
mesh_terms: D000595:Amino Acid Sequence; D000704:Analysis of Variance; D000818:Animals; D000835:Animals, Wild / Q000502:physiology; D018949:Antigens, CD1 / Q000502:physiology; D001522:Behavior, Animal; D015180:Electrophoresis, Gel, Two-Dimensional; D015151:Immunoblotting; D007150:Immunohistochemistry; D007858:Learning; D008297:Male; D008568:Memory / Q000502:physiology*; D051379:Mice; D008815:Mice, Inbred Strains / Q000502:physiology*; D008969:Molecular Sequence Data; D009812:Odorants; D009830:Olfactory Bulb / Q000737:chemistry / Q000378:metabolism*; D010446:Peptide Fragments / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D054645:Protein Phosphatase 1 / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D012903:Smell / Q000502:physiology*
chemical_list: D018949:Antigens, CD1; D010446:Peptide Fragments; D054645:Protein Phosphatase 1