title: [Identification of a novel allele HLA-B*15:129 by polymerase chain reaction with allele group-specific primers].
abstract: OBJECTIVE
  To analyze the sequence of the exons 2-4 of human leukocyte antigen (HLA) novel allele HLA-B*15:129.
  
  
  METHODS
  DNA of the proband was extracted from whole blood by commercial DNA extraction kit. The amplification for HLA-B exons 2-4 was performed separately by polymerase chain reaction (PCR) with allele group specific primers. The PCR products were digested with enzymes and then directly sequenced for exons 2-4 of HLA-B locus in both directions.
  
  
  RESULTS
  Sequencing results showed the HLA-B alleles of the proband included B*07:02 and a novel allele. The sequence of the novel allele has been submitted to GenBank (accession no. EF473219) and the allele has been officially named B*15:129 by the WHO Nomenclature Committee. Comparing with the HLA-B*15:01:01:01, the sequence of exons 2-4 of HLA-B*15:129 showed three nucleotide difference in exon 3 at positions 362 and 363 from GG to AT and positions 369 from C to T, which resulted in an amino acid change from Arg to Asn at codon 97.
  
  
  CONCLUSIONS
  A novel HLA-B allele was identified and has been officially named B15:129 by the WHO Nomenclature Committee.
authors.affiliation: Blood Center of Zhejiang Province, Key Laboratory of Blood Safety of Ministry of Health, Hangzhou, Zhejiang, 310006 P. R. China.
authors.forename: Zhe-dong
authors.initials: ZD
authors.lastname: Han
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Jun-jun
authors.initials: JJ
authors.lastname: He
authors.forename: Nan-ying
authors.initials: NY
authors.lastname: Chen
authors.forename: Fa-ming
authors.initials: FM
authors.lastname: Zhu
authors.forename: Hang-jun
authors.initials: HJ
authors.lastname: Lv
authors.forename: Li-xing
authors.initials: LX
authors.lastname: Yan
mesh_terms: D000483:Alleles; D001483:Base Sequence; D017931:DNA Primers; D005091:Exons; D015235:HLA-B Antigens / Q000235:genetics*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D058889:Molecular Typing; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D015235:HLA-B Antigens