title: Anammox bacteria community and nitrogen removal in a strip-like wetland in the riparian zone.
abstract: A strip-like wetland was constructed in the riparian zone for investigation of ammonium nitrogen (NH(3)-N) removal in the Peach River. An inner zeolite layer was set in the wetland to adsorb NH(3)-N and further to remove total nitrogen (TN). An oxygen-deficient condition with dissolved oxygen of 0.87-1.60 mg L(-1) was observed in the zeolite layer, which benefits anaerobic ammonium oxidation (anammox) bacteria survival. The community structure of anammox bacteria was analyzed in the zeolite layer. The analysis shows that the anammox bacterial sequences are grouped into three known distinct clusters: Candidatus Brocadia fulgida, Candidatus Brocadia anammoxidans and Candidatus Jettenia asiatica. The intensified test driven by artificial pumping shows that average removal rates of NH(3)-N and TN are 41.6 mg m(-3)d(-1) and 63.2 mg m(-3)d(-1), respectively. The normal test driven by natural hydrodynamics also verifies that NH(3)-N removal mainly happens in the zeolite layer. Microbial mechanism of TN removal in the wetland involves both the autotrophic and heterotrophic process. These results suggest that the strip-like RW can be a cost-effective approach for NH(3)-N removal and can potentially be extended to similar rivers as no extra energy is required to maintain the wetland operation.
authors.affiliation: The Key Laboratory of Water and Sediment Sciences, Ministry of Education, School of Environment, Beijing Normal University, Beijing, PR China. yspei@bnu.edu.cn
authors.forename: Yuansheng
authors.initials: Y
authors.lastname: Pei
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Ziyuan
authors.initials: Z
authors.lastname: Wang
authors.forename: Binghui
authors.initials: B
authors.lastname: Tian
mesh_terms: D000693:Anaerobiosis; D001421:Bacteria, Anaerobic / Q000145:classification / Q000254:growth & development / Q000378:metabolism*; D001673:Biodegradation, Environmental; D009584:Nitrogen / Q000032:analysis / Q000378:metabolism*; D000644:Quaternary Ammonium Compounds / Q000032:analysis / Q000378:metabolism*; D045483:Rivers / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000378:metabolism*; D053833:Wetlands
chemical_list: D000644:Quaternary Ammonium Compounds; D014874:Water Pollutants, Chemical; D009584:Nitrogen