title: T₂ -weighted MRI of post-infarct myocardial edema in mice.
abstract: T(2) -weighted, cardiac magnetic resonance imaging (T(2) w CMR) can be used to noninvasively detect and quantify the edematous region that corresponds to the area at risk (AAR) following myocardial infarction (MI). Previously, CMR has been used to examine structure and function in mice, expediting the study of genetic manipulations. To date, CMR has not been applied to imaging of post-MI AAR in mice. We developed a whole-heart, T(2) w CMR sequence to quantify the AAR in mouse models of ischemia and infarction. The ΔB(0) and ΔB(1) environment around the mouse heart at 7 T were measured, and a T(2) -preparation sequence suitable for these conditions was developed. Both in vivo T(2) w and late gadolinium enhanced CMR were performed in mice after 20-min coronary occlusions, resulting in measurements of AAR size of 32.5 ± 3.1 (mean ± SEM)% left ventricular mass, and MI size of 50.1 ± 6.4% AAR size. Excellent interobserver agreement and agreement with histology were also found. This T(2) w imaging method for mice may allow for future investigations of genetic manipulations and novel therapies affecting the AAR and salvaged myocardium following reperfused MI.
authors.affiliation: Department of Biomedical Engineering, University of Virginia, Charlottesville, Virginia, USA.
authors.forename: Ronald J
authors.initials: RJ
authors.lastname: Beyers
authors.forename: R Scott
authors.initials: RS
authors.lastname: Smith
authors.forename: Yaqin
authors.initials: Y
authors.lastname: Xu
authors.forename: Bryan A
authors.initials: BA
authors.lastname: Piras
authors.forename: Michael
authors.initials: M
authors.lastname: Salerno
authors.forename: Stuart S
authors.initials: SS
authors.lastname: Berr
authors.forename: Craig H
authors.initials: CH
authors.lastname: Meyer
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Kramer
authors.forename: Brent A
authors.initials: BA
authors.lastname: French
authors.forename: Frederick H
authors.initials: FH
authors.lastname: Epstein
mesh_terms: D000818:Animals; D004489:Edema, Cardiac / Q000209:etiology* / Q000473:pathology*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009203:Myocardial Infarction / Q000150:complications* / Q000473:pathology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity