title: Ferrocenemonocarboxylic-HRP@Pt nanoparticles labeled RCA for multiple amplification of electro-immunosensing.
abstract: A multiple amplification immunoassay was proposed to detect alpha-fetoprotein (AFP), which was based on ferrocenemonocarboxylic-HRP conjugated on Pt nanoparticles as labels for rolling circle amplification (RCA). Firstly, the capture antibody (anti-AFP) was immobilized on glass carbon electrode (GCE) deposited nano-sized gold particles. After a typical immuno-sandwich protocol, primary DNA was immobilized by labeling secondary antibody, which acted as a precursor to initiate RCA. The products of RCA provide large amount of sites to link detection DNAs, which were labeled by signal probes (ferrocenemonocarboxylic) and horseradish peroxidase (HRP). Moreover, the enzymatic amplification signals could be produced by the catalysis of HRP and Pt nanoparticles with the addition of H₂O₂. These lead to multiple amplification signals monitoring by electrochemical instrument and further resulted in high sensitivity of the immunoassay with the detection limit of 1.7 pg/mL.
authors.affiliation: Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, College of Chemistry and Chemical Engineering, Chongqing 400715, People's Republic of China.
authors.forename: Huilan
authors.initials: H
authors.lastname: Su
authors.forename: Ruo
authors.initials: R
authors.lastname: Yuan
authors.forename: Yaqin
authors.initials: Y
authors.lastname: Chai
authors.forename: Li
authors.initials: L
authors.lastname: Mao
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhuo
mesh_terms: D000911:Antibodies, Monoclonal; D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods* / Q000706:statistics & numerical data; D015342:DNA Probes / Q000235:genetics; D055664:Electrochemical Techniques; D005296:Ferrous Compounds; D006735:Horseradish Peroxidase; D006801:Humans; D006861:Hydrogen Peroxide; D007118:Immunoassay / Q000379:methods; D053768:Metal Nanoparticles; D000075163:Metallocenes; D010984:Platinum; D000509:alpha-Fetoproteins / Q000032:analysis / Q000276:immunology
chemical_list: C097353:AFP protein, human; D000911:Antibodies, Monoclonal; D015342:DNA Probes; D005296:Ferrous Compounds; D000075163:Metallocenes; D000509:alpha-Fetoproteins; C032949:ferrocenecarboxylic acid; D010984:Platinum; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase