title: Gibberellins negatively regulate low temperature-induced anthocyanin accumulation in a HY5/HYH-dependent manner.
abstract: Low temperature could significantly induce anthocyanin accumulation in the presence of light. Recently, two bZIP transcription factors LONG HYPOCOTYL 5 (HY5) and HOMOLOG OF HY5 (HYH) were identified to play an important role in the process of low temperature-induced anthocyanin accumulation. However, the mechanism by which HY5/HYH regulates anthocyanin accumulation under low temperature still remains unclear. Here, we found that the gibberellins (GAs) could decrease but PAC (endogenous GAs biosynthesis inhibitor) increase the low temperature-induced anthocyanin accumulation, implying that GAs signaling may involve in this process. Furthermore, the transcript levels of GA2ox1, encoding a major member of bioactive GAs-deactivating enzymes, were significantly up-regulated by low temperature in a HY5/HYH-dependent manner. Moreover, hy5hyh mutant was insensitive to PAC in enhancing anthocyanin accumulation under low temperature. From these data we propose that, together with HY5/HYH, GA signaling may play an important role during low temperature-induced anthocyanin accumulation.
authors.affiliation: School of Life Sciences, Lanzhou University, Lanzhou Gansu, China.
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zhongjuan
authors.initials: Z
authors.lastname: Liu
authors.forename: Rongzhi
authors.initials: R
authors.lastname: Liu
authors.forename: Hualing
authors.initials: H
authors.lastname: Hao
authors.forename: Yurong
authors.initials: Y
authors.lastname: Bi
mesh_terms: D000872:Anthocyanins / Q000378:metabolism; D017360:Arabidopsis / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D050976:Basic-Leucine Zipper Transcription Factors / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D003080:Cold Temperature; D004268:DNA-Binding Proteins; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D005875:Gibberellins / Q000494:pharmacology; D008954:Models, Biological; D009687:Nuclear Proteins / Q000378:metabolism*
chemical_list: D000872:Anthocyanins; D029681:Arabidopsis Proteins; D050976:Basic-Leucine Zipper Transcription Factors; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D005875:Gibberellins; C109254:HY5 protein, Arabidopsis; C457459:HYH protein, Arabidopsis; D009687:Nuclear Proteins