title: In situ spectral imaging of marker proteins in gastric cancer with near-infrared and visible quantum dots probes.
abstract: This study presents the investigation of bioconjugating ability of near-infrared (NIR) CdSeTe/ZnS quantum dots (QDs) (710 nm) and visible CdSe QDs (595 nm) in immunofluorescent staining for cancer biomarkers in gastric cancer tissues probed with the homemade Hadamard transform (HT) spectral imaging microscope and a commercial multispectral imaging system. The results show that imunostaining ability of NIR QDs probes is stronger than that of visible QDs when the two kinds of QDs are simultaneously used to probe the cancer biomarkers such as cytokeratin 20 (CK20) and proliferating cell nuclear antigen (PCNA) in gastric cancer tissues. Moreover, when the two QDs probes are used for immunostaining successively for the same target molecules, staining order has great influences on the final results due to their different conjugating ability to the marker proteins. The results imply that NIR QDs hold more promise for real-time imaging of tumor tissues due to its higher sensitivity and contrast. In addition, the results also demonstrate the potential of Hadamard transform spectral imaging as a useful tool in biomedical analysis and quantitative evaluation for tumor tissues.
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Research Center for Nanobiology and Nanomedicine (MOE 985 Innovative Platform), and State Key Laboratory of Virology, Wuhan University, Wuhan 430072, P. R. China.
authors.forename: Yue
authors.initials: Y
authors.lastname: He
authors.forename: Hao
authors.initials: H
authors.lastname: Xu
authors.forename: Chuang
authors.initials: C
authors.lastname: Chen
authors.forename: Jun
authors.initials: J
authors.lastname: Peng
authors.forename: Hongwu
authors.initials: H
authors.lastname: Tang
authors.forename: Zhiling
authors.initials: Z
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Daiwen
authors.initials: D
authors.lastname: Pang
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis; D003952:Diagnostic Imaging / Q000379:methods*; D015200:Fluoroimmunoassay; D006801:Humans; D007259:Infrared Rays; D008027:Light; D008856:Microscopy, Fluorescence; D009363:Neoplasm Proteins / Q000032:analysis*; D045663:Quantum Dots; D013274:Stomach Neoplasms / Q000175:diagnosis* / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins