title: Manipulating corn germplasm to increase recombinant protein accumulation.
abstract: Using plants as biofactories for industrial enzymes is a developing technology. The application of this technology to plant biomass conversion for biofuels and biobased products has potential for significantly lowering the cost of these products because of lower enzyme production costs. However, the concentration of the enzymes in plant tissue must be high to realize this goal. We describe the enhancement of the accumulation of cellulases in transgenic maize seed as a part of the process to lower the cost of these dominant enzymes for the bioconversion process. We have used breeding to move these genes into elite and high oil germplasm to enhance protein accumulation in grain. We have also explored processing of the grain to isolate the germ, which preferentially contains the enzymes, to further enhance recovery of enzyme on a dry weight basis of raw materials. The enzymes are active on microcrystalline cellulose to release glucose and cellobiose.
authors.affiliation: Arkansas State University, Jonesboro, AR, USA. ehood@astate.edu
authors.forename: Elizabeth E
authors.initials: EE
authors.lastname: Hood
authors.forename: Shivakumar P
authors.initials: SP
authors.lastname: Devaiah
authors.forename: Gina
authors.initials: G
authors.lastname: Fake
authors.forename: Erin
authors.initials: E
authors.lastname: Egelkrout
authors.forename: Keat Thomas
authors.initials: KT
authors.lastname: Teoh
authors.forename: Deborah Vicuna
authors.initials: DV
authors.lastname: Requesens
authors.forename: Celine
authors.initials: C
authors.lastname: Hayden
authors.forename: Kendall R
authors.initials: KR
authors.lastname: Hood
authors.forename: Kameshwari M
authors.initials: KM
authors.lastname: Pappu
authors.forename: Jennifer
authors.initials: J
authors.lastname: Carroll
authors.forename: John A
authors.initials: JA
authors.lastname: Howard
mesh_terms: D018533:Biomass; D001947:Breeding; D002241:Carbohydrates / Q000032:analysis; D002480:Cellulase / Q000302:isolation & purification / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003433:Crosses, Genetic; D004591:Electrophoresis, Polyacrylamide Gel; D018628:Gene Dosage / Q000235:genetics; D005818:Genetic Engineering / Q000379:methods*; D006824:Hybridization, Genetic; D030821:Plants, Genetically Modified; D011994:Recombinant Proteins / Q000378:metabolism*; D012639:Seeds / Q000201:enzymology / Q000378:metabolism*; D013379:Substrate Specificity; D019076:Transgenes / Q000235:genetics; D003313:Zea mays / Q000235:genetics* / Q000378:metabolism*
chemical_list: D002241:Carbohydrates; D011994:Recombinant Proteins; D002482:Cellulose; D002480:Cellulase