title: [Analytical evaluation of mono[{3-[4-(2-etoxyetoxy)-benzoyloxy]-2-hydroxypropyl}-isopropylammonium]fumarate].
abstract: The present paper deals with a complex spectral and physicochemical evaluation of mono[{3-[4-(2-etoxyetoxy)-benzoyloxy]-2-hydroxypropyl}-isopropylammonium]fumarate, a potential ultrashort acting beta1-blocker. The identity of the substance under study (labelled as UPB-1) was confirmed by 1H- and 13C-NMR spectra as well as IR spectrometry. The determined fundamental physicochemical characteristics included the determination of the melting point, solubility in a spectrum of solvents, verification of purity (adsorption thin-layer chromatography), determination of surface activity (Traube's stalagmometric method), acidobasic characteristics (pK(a) value by means of alkalimetric titration), determination of log epsilon values using spectrophotometry in UV/VIS region, as well as the evaluation of the effect of acid and basic media on the stability of the substance under the study. Other experimentally determined parameters were lipohydrophilic characteristics essayed by means of RP-HPLC (log k'), and the shake-flask method was employed to determine the values of the partition coefficients P(exp) (resp. log P(exp)) in different lipohydrophilic media. On the basis of log P(exp-) data, the ability of the substance to penetrate the hematoencephalic barrier was predicted. To determine the UPB-1 content, RP-HPLC (reversed-phase HPLC) method of the internal standard and UV/VIS spectrophotometry at the wavelength of 260 nm (aqueous medium) and 258 nm (methanol medium) were used.
authors.affiliation: Univerzita Komenského v Bratislave, Farmaceutická fakulta, Katedra farmaceutickej chémie, Slovenská republika. malik@fpharm.uniba.sk
authors.forename: Ivan
authors.initials: I
authors.lastname: Malík
authors.forename: Fils
authors.initials: F
authors.lastname: Andriamainty
authors.forename: Eva
authors.initials: E
authors.lastname: Sedlárová
authors.forename: Jozef
authors.initials: J
authors.lastname: Cizmárik
authors.forename: Jana
authors.initials: J
authors.lastname: Galisinová
authors.forename: Petr
authors.initials: P
authors.lastname: Mokrý
authors.forename: Jozef
authors.initials: J
authors.lastname: Csöllei
authors.forename: Janka
authors.initials: J
authors.lastname: Karlovská
authors.forename: Milos
authors.initials: M
authors.lastname: Lukác
mesh_terms: D058671:Adrenergic beta-1 Receptor Antagonists / Q000737:chemistry*; D002626:Chemistry, Pharmaceutical; D002627:Chemistry, Physical; D009682:Magnetic Resonance Spectroscopy; D013055:Spectrophotometry, Infrared; D013057:Spectrum Analysis
chemical_list: D058671:Adrenergic beta-1 Receptor Antagonists