title: The community dynamics of major bioleaching microorganisms during chalcopyrite leaching under the effect of organics.
abstract: To determine the effect of organics (yeast extract) on microbial community during chalcopyrite bioleaching at different temperature, real-time polymerase chain reaction (PCR) was employed to analyze community dynamics of major bacteria applied in bioleaching. The results showed that yeast extract exerted great impact on microbial community, and therefore influencing bioleaching rate. To be specific, yeast extract was adverse to this bioleaching process at 30°C due to decreased proportion of important chemolithotrophs such as Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans. However, yeast extract could promote bioleaching rate at 40°C on account of the increased number and enhanced work of Ferroplasma thermophilum, a kind of facultative bacteria. Similarly, bioleaching rate was enhanced under the effect of yeast extract at 50°C owing to the work of Acidianus brierleyi. At 60°C, bioleaching rate was close to 100% and temperature was the dominant factor determining bioleaching rate. Interestingly, the existence of yeast extract greatly enhanced the relative competitiveness of Ferroplasma thermophilum in this complex bioleaching microbial community.
authors.affiliation: School of Metallurgical Science and Engineering, Central South University, Changsha, Hunan, China.
authors.forename: Qihou
authors.initials: Q
authors.lastname: Li
authors.forename: Ye
authors.initials: Y
authors.lastname: Tian
authors.forename: Xian
authors.initials: X
authors.lastname: Fu
authors.forename: Huaqun
authors.initials: H
authors.lastname: Yin
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Zhou
authors.forename: Yiting
authors.initials: Y
authors.lastname: Liang
authors.forename: Guanzhou
authors.initials: G
authors.lastname: Qiu
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.forename: Hongwei
authors.initials: H
authors.lastname: Liu
authors.forename: Yili
authors.initials: Y
authors.lastname: Liang
authors.forename: Li
authors.initials: L
authors.lastname: Shen
authors.forename: Jing
authors.initials: J
authors.lastname: Cong
authors.forename: Xueduan
authors.initials: X
authors.lastname: Liu
mesh_terms: D001419:Bacteria / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism*; D044822:Biodiversity; D016000:Cluster Analysis; D003300:Copper / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D027081:DNA Gyrase / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013696:Temperature
chemical_list: D003470:Culture Media; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; C012819:chalcopyrite; D003300:Copper; D027081:DNA Gyrase