title: The autoregulator receptor homologue AvaR3 plays a regulatory role in antibiotic production, mycelial aggregation and colony development of Streptomyces avermitilis.
abstract: The γ-butyrolactone autoregulator receptor has been shown to control secondary metabolism and/or morphological differentiation across many Streptomyces species. Streptomyces avermitilis produces an important anthelmintic agent (avermectin) and two further polyketide antibiotics, filipin and oligomycin. Genomic analysis of S. avermitilis revealed that this micro-organism has the clustered putative autoregulator receptor genes distant from the antibiotic biosynthetic gene clusters. Here, we describe the characterization of avaR3, one of the clustered receptor genes, which encodes a protein containing an extra stretch of amino acid residues that has not been found in the family of autoregulator receptors. Disruption of avaR3 resulted in markedly decreased production of avermectins, with delayed expression of avermectin biosynthetic genes, suggesting that AvaR3 positively controls the avermectin biosynthetic genes. Moreover, the disruption caused increased production of filipin without any changes in the transcriptional profile of the filipin biosynthetic genes, suggesting that filipin production is indirectly controlled by AvaR3. The avaR3 disruptant displayed fragmented growth in liquid culture and conditional morphological defects on solid medium. These findings demonstrated that AvaR3 acts as a global regulator that controls antibiotic production and cell morphology.
authors.affiliation: International Center for Biotechnology, Osaka University, 2-1, Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Kiyoko T
authors.initials: KT
authors.lastname: Miyamoto
authors.affiliation: International Center for Biotechnology, Osaka University, 2-1, Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Kitani
authors.affiliation: Kitasato Institute for Life Sciences, Kitasato University, 1-15-1, Kitasato, Sagamihara, Kanagawa 252-0373, Japan.
authors.forename: Mamoru
authors.initials: M
authors.lastname: Komatsu
authors.affiliation: Kitasato Institute for Life Sciences, Kitasato University, 1-15-1, Kitasato, Sagamihara, Kanagawa 252-0373, Japan.
authors.forename: Haruo
authors.initials: H
authors.lastname: Ikeda
authors.affiliation: MU-OU Collaborative Research Center for Bioscience and Biotechnology, Faculty of Science, Mahidol University, Rama VI Rd, Bangkok 10400, Thailand.|International Center for Biotechnology, Osaka University, 2-1, Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Nihira
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D053898:Biosynthetic Pathways / Q000235:genetics; D003470:Culture Media / Q000737:chemistry; D005372:Filipin / Q000096:biosynthesis*; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D055786:Gene Knockout Techniques; D005798:Genes, Bacterial; D007559:Ivermectin / Q000031:analogs & derivatives* / Q000378:metabolism; D005810:Multigene Family; D016254:Mutagenesis, Insertional; D013302:Streptomyces / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D003470:Culture Media; D007559:Ivermectin; C019264:avermectin; D005372:Filipin