title: An inexpensive high-throughput nuclear magnetic resonance tube cleaning apparatus.
abstract: Large-scale nuclear magnetic resonance (NMR) tube cleaning is currently a bottleneck in high-throughput NMR ligand affinity screens. Expensive alternatives include discarding the NMR tubes after a single use (~US $2-$8/tube), using commercial NMR tube cleaners (~$15,000), and abandoning NMR tubes for flow probe technology (~$75,000). Instead, we describe a relatively inexpensive (~$400) and easily constructed apparatus that can clean 180 NMR tubes per hour while using a modest amount of solvent. The application of this apparatus significantly shortens the time to recycle NMR tubes while avoiding cross-contamination and tube damage.
authors.affiliation: Department of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.forename: James
authors.initials: J
authors.lastname: Hodgson
authors.forename: Walter
authors.initials: W
authors.lastname: Hancock
authors.forename: Robert
authors.initials: R
authors.lastname: Powers
mesh_terms: D000096:Acetone / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000191:economics / Q000295:instrumentation*; D000432:Methanol / Q000737:chemistry; D017942:Nitric Acid / Q000737:chemistry; D059027:Recycling / Q000191:economics / Q000379:methods*; D017693:Sodium Bicarbonate / Q000737:chemistry; D012997:Solvents / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D012997:Solvents; D014867:Water; D000096:Acetone; D017942:Nitric Acid; D017693:Sodium Bicarbonate; D000432:Methanol