title: Taenia solium: a two-dimensional Western blotting method combined with the use of an EST-library for the identification of immunogenic proteins recognized by sera from neurocysticercosis patients.
abstract: Commercial antigens used to diagnose human neurocysticercosis (NCC) are obtained from either a soluble parasite extract or a parasite-derived glycoprotein fraction. The aim of the present study was to identify antigenic proteins as potential diagnostic candidates in this context. Soluble immunogenic proteins from Taenia solium cysticerci were identified by two-dimensional electrophoresis Western blotting using human sera from Nicaragua confirmed to be positive for NCC by computer tomography. Six antigenic proteins were identified and sequenced by liquid chromatography-mass spectrometry. Among these immunogenic proteins, a novel sequence was found and named Tsol-p27. To determine the antigenicity of Tsol-p27, the previously reported antigen TsolHSP36 and the new Tsol-p27 were expressed as recombinant proteins and evaluated serologically. Immunoblotting demonstrated that Tsol-p27 was recognized by sera from 13 NCC-positive humans, whereas TsolHSP36 was identified by only two of those 13 positive sera. None of the antigens were recognized by negative control sera. Despite the limited number of serum samples evaluated in this study, the results indicate that Tsol-p27 might be a suitable candidate for diagnosis of human NCC.
authors.affiliation: Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, P.O. Box 280, SE-171 77 Stockholm, Sweden. fernando.salazar@smi.se
authors.forename: Fernando
authors.initials: F
authors.lastname: Salazar-Anton
authors.forename: Johan
authors.initials: J
authors.lastname: Lindh
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology; D000947:Antigens, Helminth / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D015153:Blotting, Western; D003001:Cloning, Molecular; D018509:DNA, Helminth / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D020224:Expressed Sequence Tags; D015723:Gene Library; D015801:Helminth Proteins / Q000032:analysis* / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D006801:Humans; D007106:Immune Sera / Q000276:immunology*; D020019:Neurocysticercosis / Q000097:blood / Q000175:diagnosis / Q000276:immunology*; D016415:Sequence Alignment; D013552:Swine; D041201:Taenia solium / Q000235:genetics / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D018509:DNA, Helminth; D015801:Helminth Proteins; D007106:Immune Sera