title: Genetic toxicology and preliminary in vivo studies of nitric oxide donor tocopherol analogs as potential new class of antiatherogenic agents.
abstract: Nitric oxide donor tocopherol analogs were found to be incorporated in low-density lipoprotein to release nitric oxide into the hydrophobic core of the lipoprotein, thus inhibiting lipid oxidation processes associated with atheroma plaque formation. Previously, we studied their cytotoxicity against human and murine macrophages as first selection for in vivo studies. Herein, we examined both the in vitro mutagenic and DNA-damage effects of selected compounds to further evaluate drug potential. While the compounds of interest were nongenotoxics in both experimental tests (Ames and alkaline comet), one of the potential blood metabolites exhibited genotoxicity (alkaline comet test), and the furazan derivative was mutagenic (Ames test). Two selected (nitrooxy and furoxan) compounds were studied in long- and short-term in vivo treatment, and in these conditions, animal toxicity was not evidenced, suggesting the possibility of these compounds as potential antiatherogenic drugs.
authors.affiliation: Laboratorio de Química Orgánica, Instituto de Química Biológica, Facultad de Ciencias-Facultad de Química, Universidad de la República, Montevideo, Uruguay.
authors.forename: Mauricio
authors.initials: M
authors.lastname: Cabrera
authors.forename: Gloria V
authors.initials: GV
authors.lastname: López
authors.forename: Luis E
authors.initials: LE
authors.lastname: Gómez
authors.forename: Martín
authors.initials: M
authors.lastname: Breijo
authors.forename: Cristina
authors.initials: C
authors.lastname: Pintos
authors.forename: Horacio
authors.initials: H
authors.lastname: Botti
authors.forename: Stella
authors.initials: S
authors.lastname: Raymondo
authors.forename: Ariane
authors.initials: A
authors.lastname: Vettorazzi
authors.forename: Adela López de
authors.initials: AL
authors.lastname: Ceráin
authors.forename: Antonio
authors.initials: A
authors.lastname: Monge
authors.forename: Homero
authors.initials: H
authors.lastname: Rubbo
authors.forename: Mercedes
authors.initials: M
authors.lastname: González
authors.forename: Hugo
authors.initials: H
authors.lastname: Cerecetto
mesh_terms: D000818:Animals; D050197:Atherosclerosis / Q000188:drug therapy*; D002460:Cell Line; D020552:Comet Assay; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007273:Injections, Intramuscular; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008862:Microsomes, Liver / Q000378:metabolism; D015394:Molecular Structure; D009153:Mutagens / Q000737:chemistry / Q000633:toxicity*; D020030:Nitric Oxide Donors / Q000737:chemistry / Q000627:therapeutic use / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics; D013329:Structure-Activity Relationship; D024505:Tocopherols / Q000737:chemistry / Q000627:therapeutic use / Q000633:toxicity*
chemical_list: D009153:Mutagens; D020030:Nitric Oxide Donors; D024505:Tocopherols