title: Ethanol production from marine algal hydrolysates using Escherichia coli KO11.
abstract: Algae biomass is a potential raw material for the production of biofuels and other chemicals. In this study, biomass of the marine algae, Ulva lactuca, Gelidium amansii,Laminaria japonica, and Sargassum fulvellum, was treated with acid and commercially available hydrolytic enzymes. The hydrolysates contained glucose, mannose, galactose, and mannitol, among other sugars, at different ratios. The Laminaria japonica hydrolysate contained up to 30.5% mannitol and 6.98% glucose in the hydrolysate solids. Ethanogenic recombinant Escherichia coli KO11 was able to utilize both mannitol and glucose and produced 0.4g ethanol per g of carbohydrate when cultured in L. japonica hydrolysate supplemented with Luria-Bertani medium and hydrolytic enzymes. The strategy of acid hydrolysis followed by simultaneous enzyme treatment and inoculation with E. coli KO11 could be a viable strategy to produce ethanol from marine alga biomass.
authors.affiliation: Department of Chemical and Biomolecular Engineering (BK21 program), KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.
authors.forename: Nag-Jong
authors.initials: NJ
authors.lastname: Kim
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Kwonsu
authors.initials: K
authors.lastname: Jung
authors.forename: Ho Nam
authors.initials: HN
authors.lastname: Chang
authors.forename: Pyung Cheon
authors.initials: PC
authors.lastname: Lee
mesh_terms: D000460:Chlorophyta / Q000737:chemistry*; D004926:Escherichia coli / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000737:chemistry / Q000378:metabolism; D006868:Hydrolysis; D007795:Laminaria / Q000737:chemistry*; D008353:Mannitol / Q000737:chemistry / Q000378:metabolism
chemical_list: D000431:Ethanol; D008353:Mannitol; D005947:Glucose