title: Examination of claudin-1 expression in patients undergoing liver transplantation owing to hepatitis C virus cirrhosis.
abstract: The cell adhesion molecule claudin-1 (CLDN-1) is a well known co-factor for the cell entry of hepatitis C virus (HCV). We examined 24 hepatic biopsies from liver transplant patients. Reverse transcriptase polymerase chain reaction (RT-PCR) and immunohistochemistry were performed according to standard procedures. RT-PCR results were shown as relative expression (ΔCT) with beta-actin as the reference gene. Immunohistochemistry results are shown by morphometry. The CLDN-1 mRNS expression rate was significantly lower when the patient displayed favorably with an unsatisfactory to antiviral therapy 0.756 ± 0.249 versus 1.304 ± 0.28 (P=.012). There was also a strong positive correlation between CLDN-1 protein expression and liver fibrosis (Pearson correlation coefficients: r=0.476; P=.034).
authors.affiliation: Clinic of Transplantation and Surgery Budapest, Budapest, Hungary. zadorig@yahoo.com
authors.forename: G
authors.initials: G
authors.lastname: Zadori
authors.forename: F
authors.initials: F
authors.lastname: Gelley
authors.forename: P
authors.initials: P
authors.lastname: Torzsok
authors.forename: E
authors.initials: E
authors.lastname: Sárváry
authors.forename: A
authors.initials: A
authors.lastname: Doros
authors.forename: A P
authors.initials: AP
authors.lastname: Deak
authors.forename: P
authors.initials: P
authors.lastname: Nagy
authors.forename: Z
authors.initials: Z
authors.lastname: Schaff
authors.forename: A
authors.initials: A
authors.lastname: Kiss
authors.forename: B
authors.initials: B
authors.lastname: Nemes
mesh_terms: D000998:Antiviral Agents / Q000627:therapeutic use; D001706:Biopsy; D062445:Claudin-1; D005260:Female; D019698:Hepatitis C, Chronic / Q000150:complications* / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism; D006801:Humans; D006814:Hungary; D007150:Immunohistochemistry; D008099:Liver / Q000378:metabolism / Q000601:surgery* / Q000821:virology; D008103:Liver Cirrhosis / Q000235:genetics / Q000378:metabolism / Q000601:surgery* / Q000821:virology; D016031:Liver Transplantation; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D012333:RNA, Messenger / Q000378:metabolism; D012008:Recurrence; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D016896:Treatment Outcome
chemical_list: D000998:Antiviral Agents; C568108:CLDN1 protein, human; D062445:Claudin-1; D008565:Membrane Proteins; D012333:RNA, Messenger