title: Detection of SRS19-6MuLV in mouse dendritic cell sarcoma and its tumorigenesis.
abstract: SRS19-6MuLV is a member of the MuLV family originally isolated from the Tianjin-Shanghai-Zunyi complex of murine leukemia. A notable characteristic of this virus is that it induces tumors of multiple hematopoietic lineages, including myeloid, erythroid, T-lymphoid and B-lymphoid. In a previous study, a sequence with high homology to SRS19-6MuLV in a murine dendritic cell sarcoma (DCS) was identified through cDNA expression screening with mAb 983D4. To investigate the relationship between SRS19-6MuLV and DCS, the existence of a specific SRS19-6MuLV DNA fragment in DCS cells, 15 murine tumor cells, 2 murine tumor tissues, 12 normal murine cells/tissues, 11 human tumor cell lines and SRSV/3T3 (NIH/3T3 cells infected with SRS cell supernatant) was detected by PCR. The specific fragment of SRS19-6MuLV was detected in DCS, mouse fore-gastric cancer cells, LⅡ tumor tissue from which DCS is derived and SRSV/3T3. In addition, the integration sites of SRS19-6MuLV in the positive cells were examined by inverse PCR. Thus, 7 integration sites for SRS19-6MuLV were detected in DCS and 3 in SRSV/3T3. Analysis of sequences by BLAST revealed that some of the integration sites were associated with common fragile sites and some Ras-regulating miRNAs. Our results indicate that SRS19-6MuLV not only induced four types of leukemia, but also induced DCS. This virus does not infect human cells. Multiple integration of SRS19-6MuLV into chromosomes around fragile sites accounts for its carcinogenic effects.
authors.affiliation: Department of Pathology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Tsinghua University, Beijing 100005, P.R. China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Liu
authors.forename: Xiaocui
authors.initials: X
authors.lastname: Bian
authors.forename: Bei
authors.initials: B
authors.lastname: Gu
authors.forename: Hailiang
authors.initials: H
authors.lastname: Feng
authors.forename: Zhenli
authors.initials: Z
authors.lastname: Yang
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Wenjing
authors.initials: W
authors.lastname: Zhao
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D019295:Computational Biology; D004279:DNA, Viral / Q000235:genetics; D003713:Dendritic Cells / Q000473:pathology* / Q000648:ultrastructure / Q000821:virology*; D006801:Humans; D009052:Leukemia Virus, Murine / Q000235:genetics*; D051379:Mice; D016133:Polymerase Chain Reaction; D011230:Precancerous Conditions / Q000473:pathology / Q000821:virology*; D012509:Sarcoma / Q000473:pathology / Q000648:ultrastructure / Q000821:virology*; D014771:Virion / Q000648:ultrastructure; D016662:Virus Integration
chemical_list: D004279:DNA, Viral