title: Antiproliferative and proapoptotic effects of bisphenol A on human trophoblastic JEG-3 cells.
abstract: Different studies performed in rodents revealed that bisphenol-A (BPA), an environmental compound, altered early embryonic development. However, little is known concerning the direct effects of BPA on human implantation process. Thus, we decided to study in vitro BPA's effects on proliferative capacities of the human trophoblastic cell line, JEG-3. For this purpose, we first have shown that JEG-3 cells express the specific BPA receptor, namely estrogen-related receptor γ1 (ERRγ1). Secondly, we demonstrated that BPA did not exert any cytotoxic action in JEG-3 cells up to 10(-6)M. Moreover [(3)H]-thymidine incorporation experiments revealed that BPA significantly reduced cell proliferation. The results also showed that BPA induced JEG-3 apoptosis capacity as reflected by DNA fragmentation experiments. In conclusion, we describe here the direct impact of BPA on trophoblastic cell number mediated through both anti-proliferative and pro-apoptotic effects.
authors.affiliation: Laboratoire de Recherche, Université de Versailles-St-Quentin, Service de Biochimie et Biologie Moléculaire, UPRES-EA 2493, Faculté de Médecine Paris-Ile de France Ouest, PRES Universud Paris, Centre Hospitalier de Poissy-Saint Germain, Rue du Champ Gaillard 78303, Poissy Cedex, France.
authors.forename: Lucie
authors.initials: L
authors.lastname: Morice
authors.forename: Delphine
authors.initials: D
authors.lastname: Benaîtreau
authors.forename: Marie-Noëlle
authors.initials: MN
authors.lastname: Dieudonné
authors.forename: Corinne
authors.initials: C
authors.lastname: Morvan
authors.forename: Valérie
authors.initials: V
authors.lastname: Serazin
authors.forename: Philippe
authors.initials: P
authors.lastname: de Mazancourt
authors.forename: René
authors.initials: R
authors.lastname: Pecquery
authors.forename: Esther
authors.initials: E
authors.lastname: Dos Santos
mesh_terms: D000293:Adolescent; D000328:Adult; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002822:Choriocarcinoma / Q000188:drug therapy* / Q000473:pathology; D053938:DNA Fragmentation; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D020868:Gene Silencing; D006801:Humans; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D034741:RNA, Small Interfering; D011960:Receptors, Estrogen / Q000235:genetics / Q000378:metabolism; D014327:Trophoblasts / Q000187:drug effects* / Q000473:pathology; D014594:Uterine Neoplasms / Q000188:drug therapy* / Q000473:pathology; D055815:Young Adult
chemical_list: C490917:ESRRG protein, human; D034741:RNA, Small Interfering; D011960:Receptors, Estrogen