title: Electric-field-induced changes in absorption and fluorescence of the green fluorescent protein chromophore in a PMMA film.
abstract: External electric field effects on absorption, fluorescence, and fluorescence decay of p-HBDI that is a model compound of the chromophore of GFP have been examined in a poly(methyl methacrylate) film. The electroabsorption spectrum is similar in shape to the first derivative of the absorption spectrum, which results from the difference in molecular polarizability between the ground state and the Franck-Condon excited state. The electrophotoluminescence spectrum is dominated by the corresponding fluorescence spectrum, indicating the enhancement of the fluorescence intensity in the presence of external electric fields. The direct measurements of the electric field effect on the fluorescence decay profile suggest that the field-induced deceleration of the nonradiative process contributes to the increase in the fluorescence intensity in the presence of electric fields.
authors.affiliation: Research Institute for Electronic Science (RIES), Hokkaido University, Sapporo, Japan. takan@es.hokudai.ac.jp
authors.forename: Takakazu
authors.initials: T
authors.lastname: Nakabayashi
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Hino
authors.forename: Yuka
authors.initials: Y
authors.lastname: Ohta
authors.forename: Sayuri
authors.initials: S
authors.lastname: Ito
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Nakano
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Ohta
mesh_terms: D000042:Absorption; D000818:Animals; D004560:Electricity; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D049452:Green Fluorescent Proteins / Q000737:chemistry*; D008422:Materials Testing; D015394:Molecular Structure; D019904:Polymethyl Methacrylate / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D049452:Green Fluorescent Proteins; D019904:Polymethyl Methacrylate