title: Overexpression of 2-cysteine peroxiredoxin enhances tolerance to methyl viologen-mediated oxidative stress and high temperature in potato plants.
abstract: Oxidative stress is one of the major causative factors for injury to plants exposed to environmental stresses. Plants have developed diverse defense mechanisms for scavenging oxidative stress-inducing molecules. The antioxidative enzyme 2-cysteine peroxiredoxin (2-Cys Prx) removes peroxides and protects the photosynthetic membrane from oxidative damage. In this study, transgenic potato (Solanum tuberosum L. cv. Atlantic) expressing At2-Cys Prx under control of the oxidative stress-inducible SWPA2 promoter or enhanced CaMV 35S promoter (referred to as SP and EP plants, respectively) was generated using Agrobacterium-mediated transformation. The transgenic plants were tested for tolerance to stress. Following treatment with 3 μM methyl viologen (MV), leaf discs from SP and EP plants showed approximately 33 and 15% less damage than non-transformed (NT) plants. When 300 μM MV was sprayed onto whole plants, the photosynthetic activity of SP plants decreased by 25%, whereas that of NT plants decreased by 60%. In addition, SP plants showed enhanced tolerance to high temperature at 42 °C. After treatment at high temperature, the photosynthetic activity of SP plants decreased by about 7% compared to plants grown at 25 °C, whereas it declined by 31% in NT plants. These results indicate that transgenic potato can efficiently regulate oxidative stress from various environmental stresses via overexpression of At2-Cys Prx under control of the stress-inducible SWPA2 promoter.
authors.affiliation: Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yusong-gu, Daejeon 305-806, Republic of Korea.
authors.forename: Myoung Duck
authors.initials: MD
authors.lastname: Kim
authors.forename: Yun-Hee
authors.initials: YH
authors.lastname: Kim
authors.forename: Suk-Yoon
authors.initials: SY
authors.lastname: Kwon
authors.forename: Bo-Young
authors.initials: BY
authors.lastname: Jang
authors.forename: Sang Yeol
authors.initials: SY
authors.lastname: Lee
authors.forename: Dae-Jin
authors.initials: DJ
authors.lastname: Yun
authors.forename: Ji-Hong
authors.initials: JH
authors.lastname: Cho
authors.forename: Sang-Soo
authors.initials: SS
authors.lastname: Kwak
authors.forename: Haeng-Soon
authors.initials: HS
authors.lastname: Lee
mesh_terms: D060054:Agrobacterium / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D006358:Hot Temperature; D018384:Oxidative Stress / Q000235:genetics*; D010269:Paraquat / Q000494:pharmacology; D054464:Peroxiredoxins / Q000235:genetics*; D010788:Photosynthesis; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D011198:Solanum tuberosum / Q000187:drug effects / Q000502:physiology*; D014170:Transformation, Genetic
chemical_list: D029681:Arabidopsis Proteins; C493631:2-Cys peroxiredoxin A, Arabidopsis; D054464:Peroxiredoxins; D010269:Paraquat