title: miRNA expression profiles in myelodysplastic syndromes reveal Epstein-Barr virus miR-BART13 dysregulation.
abstract: Recently, the microRNA (miRNA) signature has been used for better characterization and understanding of the pathogenesis of different malignancies, including myelodysplastic syndromes (MDS). MDS are a heterogeneous group of stem cell disorders in which the genetic and molecular defects are not well defined. In the present study, we applied array based miRNA profiling to study 19 bone marrow cell samples of de novo MDS compared with eight healthy individuals. In addition, integration of the miRNA profiling data with our previous array comparative genomic hybridization data, from the same cohort of patients, was performed. We observed up-regulation of hsa-miR-720 and hsa-miR-21, and down-regulation of hsa-miR-671-5p and one human virus miRNA (Epstein-Barr virus miR-BART13) in MDS samples compared with normal samples. In our study, the copy number alteration harboring miRNA was not affecting miRNA expression, but a distinct microRNA expression pattern was observed, not only in MDS compared with controls, but also between MDS entities.
authors.affiliation: Department of Pathology, Haartman Institute and HUSLAB, University of Helsinki and Helsinki University Central Hospital, Helsinki, Finland.
authors.forename: Ioana
authors.initials: I
authors.lastname: Borze
authors.forename: Ilari
authors.initials: I
authors.lastname: Scheinin
authors.forename: Sanna
authors.initials: S
authors.lastname: Siitonen
authors.forename: Erkki
authors.initials: E
authors.lastname: Elonen
authors.forename: Eeva
authors.initials: E
authors.lastname: Juvonen
authors.forename: Sakari
authors.initials: S
authors.lastname: Knuutila
mesh_terms: D001854:Bone Marrow Cells / Q000378:metabolism* / Q000821:virology; D055028:Comparative Genomic Hybridization; D015536:Down-Regulation; D020869:Gene Expression Profiling; D015967:Gene Expression Regulation, Viral; D004854:Herpesvirus 4, Human / Q000235:genetics; D006801:Humans; D007621:Karyotyping; D035683:MicroRNAs / Q000235:genetics*; D009190:Myelodysplastic Syndromes / Q000235:genetics* / Q000821:virology; D020411:Oligonucleotide Array Sequence Analysis; D015854:Up-Regulation
chemical_list: C524940:MIRN21 microRNA, human; C568637:MIRN671 microRNA, human; C568636:MIRN720 microRNA, human; D035683:MicroRNAs