title: LecT-Hepa: A triplex lectin-antibody sandwich immunoassay for estimating the progression dynamics of liver fibrosis assisted by a bedside clinical chemistry analyzer and an automated pretreatment machine.
abstract: BACKGROUND
  A quantitative analysis of glyco-alteration in serum glycoproteins provides glyco-parameters for estimating the progression of liver fibrosis. In the analysis of glycans, a manual pretreatment process for clinical specimens leads to a complicated manipulation and loss-of-clinical implementation of the assay.
  
  
  METHODS
  We evaluated an automated triplex lectin-antibody sandwich immunoassay assisted by an automated protein purification system (ED-01) and a bedside clinical chemistry analyzer (HISCL) for the acquisition of two glyco-parameters (AOL/DSA and MAL/DSA) derived from a fibrosis-related glyco-alteration of serum alpha1-acid glycoprotein (AGP).
  
  
  RESULTS
  We adjusted the auto-machines with their accuracy set to CV <5.0% (ED-01) and <1.0% (HISCL). AGP samples were enriched from 275 serum specimens. Two glyco-parameters obtained by HISCL showed a linear correlation with that from a reported assay (R>0.90). The formula for monitoring fibrosis (LecT-Hepa) was given by a combination of the glyco-parameters. This correlated with the fibrosis stage from biopsy (R=0.68) and diagnosed severe fibrosis and cirrhosis. It was superior to that of FIB-4 index.
  
  
  CONCLUSIONS
  We automated a multilectin-assisted immunoassay with an order of magnitude reduction of operation time without any loss-of-accuracy. LecT-Hepa is a reliable method to assess fibrosis-dynamics from moderate fibrosis to cirrhosis.
authors.affiliation: Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kuno
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Ikehara
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Kozue
authors.initials: K
authors.lastname: Saito
authors.forename: Kiyoaki
authors.initials: K
authors.lastname: Ito
authors.forename: Chikayuki
authors.initials: C
authors.lastname: Tsuruno
authors.forename: Shinya
authors.initials: S
authors.lastname: Nagai
authors.forename: Youichi
authors.initials: Y
authors.lastname: Takahama
authors.forename: Masashi
authors.initials: M
authors.lastname: Mizokami
authors.forename: Jun
authors.initials: J
authors.lastname: Hirabayashi
authors.forename: Hisashi
authors.initials: H
authors.lastname: Narimatsu
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood; D018450:Disease Progression; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D047468:Immunoprecipitation; D037102:Lectins; D008103:Liver Cirrhosis / Q000097:blood / Q000473:pathology*; D008875:Middle Aged; D019095:Point-of-Care Systems
chemical_list: D015415:Biomarkers; D037102:Lectins