title: High genetic diversity of HIV-1 was found in men who have sex with men in Shijiazhuang, China.
abstract: Men who have sex with men (MSM) have become one of the populations with severely HIV prevalence in China. However, very few genetic studies have been done on HIV-1 spreading in this population. In this study, the genetic characterization of HIV-1 strains prevalent in the MSM in Shijiazhuang, China, was analyzed basing on the HIV-1 full-length gag, pol, and partial env gene. 21 drug-naïve HIV-1 sero-positive patients were enrolled into the study. Full length gag, pol, partial env genes and some near full length genomes were amplified with nest RT-PCR followed by sequencing. Multiple subtypes, including CRF01_AE (52.9%), subtype B (35.3%) and CRF07_BC (11.8%), were found in the population. Phylogenetic analysis showed close relationship between our strains with those from Beijing MSM but not those from paid blood donors of local area. Drug resistance surveillance evaluation showed no transmitted drug resistance (TDR). However, some drug-resistance related polymorphism sites (V181I and V106I) were popularly found in B subtype strains. The findings will contribute to understanding the distribution and evolution of HIV-1 in MSM and also help the behavior change intervention.
authors.affiliation: Department of AIDS Research, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.forename: Xinli
authors.initials: X
authors.lastname: Lu
authors.forename: Hanping
authors.initials: H
authors.lastname: Li
authors.forename: Lili
authors.initials: L
authors.lastname: Chen
authors.forename: Zheng
authors.initials: Z
authors.lastname: Wang
authors.forename: Yongjian
authors.initials: Y
authors.lastname: Liu
authors.forename: Zuoyi
authors.initials: Z
authors.lastname: Bao
authors.forename: Tianyi
authors.initials: T
authors.lastname: Li
authors.forename: Chong
authors.initials: C
authors.lastname: Tian
authors.forename: Hongxin
authors.initials: H
authors.lastname: Liu
authors.forename: Daomin
authors.initials: D
authors.lastname: Zhuang
authors.forename: Siyang
authors.initials: S
authors.lastname: Liu
authors.forename: Jingyun
authors.initials: J
authors.lastname: Li
mesh_terms: D000328:Adult; D002681:China; D014644:Genetic Variation; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000145:classification / Q000235:genetics*; D018451:Homosexuality, Male; D054298:Human Immunodeficiency Virus Proteins / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010802:Phylogeny; D017423:Sequence Analysis, RNA; D055815:Young Adult
chemical_list: D054298:Human Immunodeficiency Virus Proteins