title: The fate of donor osteocytes in fine particulate bone powders during repair of bone defects in experimental rats.
abstract: The aim of the study was to investigate the fate of donor osteocytes in fine particulate bone powders during repair of bone defects in experimental rats. The iliac bone of male inbred DA rats was harvested and used as the larger bone grafts and also prepared as fine particulate (granulated) bone powders (300-500μm size particles) for transplantation into radial defects in female rats. The presence and relative amounts of genes specific to the sex-determining region of the Y-chromosome (Sry) originating from the bone grafts were evaluated by polymerase chain reaction and by in situ hybridization, respectively. Additional samples were evaluated histologically. In the larger bone grafts, the expression of Sry decreased relatively early, disappeared by 1 week, reappeared at 4 weeks and continued to increase with time. In the fine particulate bone powders, Sry was detected all the time and its expression was statistically greater than in the larger bone grafts at each time point. Both bone grafts provided donor cells to repair the defects. The donor cells seemed to function differently between the two groups. The fine particulate bone powders contained more living osteocytes in comparison with the larger bone grafts and may accelerate the healing of bone defects compared with conventional autografts.
authors.affiliation: Department of Orthopedic Surgery, The First Affiliated Hospital Of Harbin Medical University, Harbin, China.
authors.forename: Xin-Tao
authors.initials: XT
authors.lastname: Wang
authors.forename: Chang-Long
authors.initials: CL
authors.lastname: Zhou
authors.forename: Jing-Long
authors.initials: JL
authors.lastname: Yan
authors.forename: Xi
authors.initials: X
authors.lastname: Yan
authors.forename: Huan-Xin
authors.initials: HX
authors.lastname: Xie
authors.forename: Cheng-Li
authors.initials: CL
authors.lastname: Sun
mesh_terms: D000818:Animals; D016025:Bone Transplantation / Q000379:methods*; D058948:Cell Tracking; D005260:Female; D015870:Gene Expression; D006085:Graft Survival; D007085:Ilium / Q000378:metabolism / Q000637:transplantation*; D017403:In Situ Hybridization; D008297:Male; D010011:Osteocytes / Q000166:cytology* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011208:Powders; D051381:Rats; D055747:SOX Transcription Factors / Q000235:genetics* / Q000378:metabolism; D012737:Sex Factors; D014184:Transplantation, Homologous; D014998:Y Chromosome / Q000235:genetics
chemical_list: D011208:Powders; D055747:SOX Transcription Factors