title: Selective HPLC Analysis of n-Alkyl Hydroperoxides up to C(18)H(38)O(2).
abstract: A series of n-alkyl hydroperoxides are separated by HPLC and detected by their postcolumn reaction with horseradish peroxidase and p-hydroxyphenylacetic acid (HPAA) to yield a fluorescent product; several secondary and tertiary hydroperoxides, some 1-hydroxyalkyl hydroperoxides, and a few branched hydroperoxides are also examined. n-Alkyl hydroperoxides up to at least C-18 react with the enzyme with only minimally reduced efficiency at greater alkyl chain length. The effects of the column, the eluent, and the pH of the sample reaching the detector are described. The detection limit with gradient elution ranges from 0.4 μmol L(-)(1) for n-hexyl hydroperoxide to 1 μmol L(-)(1) for n-octadecyl hydroperoxide.
authors.affiliation: GSF [Formula: see text] Institut für Ökologische Chemie, Schulstrasse 10, 85356 Freising-Attaching, Germany, Fraunhofer [Formula: see text] Institut für Umweltchemie und Ökotoxikologie, Auf dem Aberg 1, 57392 Schmallenberg, Germany, GSF [Formula: see text] Institut für Biochemische Pflanzenpathologie, Ingolstädter Landstrasse 1, 85764 Oberschleissheim, Germany, and Analytische Chemie, Bergische Universität, Wuppertal, 42119 Wuppertal, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Heinmöller
authors.forename: H H
authors.initials: HH
authors.lastname: Kurth
authors.forename: R
authors.initials: R
authors.lastname: Rabong
authors.forename: W V
authors.initials: WV
authors.lastname: Turner
authors.forename: A
authors.initials: A
authors.lastname: Kettrup
authors.forename: S
authors.initials: S
authors.lastname: Gäb