title: Mitochondrial ubiquitin ligase MARCH5 promotes TLR7 signaling by attenuating TANK action.
abstract: The signaling of Toll-like receptors (TLRs) is the host's first line of defense against microbial invasion. The mitochondrion is emerging as a critical platform for antiviral signal transduction. The regulatory role of mitochondria for TLR signaling remains to be explored. Here, we show that the mitochondrial outer-membrane protein MARCH5 positively regulates TLR7 signaling. Ectopic expression or knockdown of MARCH5 enhances or impairs NF-κB-mediated gene expression, respectively. MARCH5 interacts specifically with TANK, and this interaction is enhanced by R837 stimulation. MARCH5 catalyzes the K63-linked poly-ubiquitination of TANK on its Lysines 229, 233, 280, 302 and 306, thus impairing the ability of TANK to inhibit TRAF6. Mislocalization of MARCH5 abolishes its action on TANK, revealing the critical role of mitochondria in modulating innate immunity. Arguably, this represents the first study linking mitochondria to TLR signaling.
authors.affiliation: Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
authors.forename: He-Xin
authors.initials: HX
authors.lastname: Shi
authors.forename: Xing
authors.initials: X
authors.lastname: Liu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
authors.forename: Pei-Pei
authors.initials: PP
authors.lastname: Tang
authors.forename: Xin-Yi
authors.initials: XY
authors.lastname: Liu
authors.forename: Yu-Fei
authors.initials: YF
authors.lastname: Shan
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000037:antagonists & inhibitors*; D000818:Animals; D016207:Cytokines / Q000032:analysis; D057809:HEK293 Cells; D006801:Humans; D007113:Immunity, Innate; D015151:Immunoblotting; D047468:Immunoprecipitation; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008928:Mitochondria / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011804:Quinolines; D034622:RNA Interference; D034741:RNA, Small Interfering; D051192:Receptors, Pattern Recognition; D015398:Signal Transduction; D048029:TNF Receptor-Associated Factor 6 / Q000037:antagonists & inhibitors / Q000378:metabolism; D051199:Toll-Like Receptor 7 / Q000819:agonists / Q000378:metabolism*; D051193:Toll-Like Receptors / Q000819:agonists / Q000378:metabolism*; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism*; D054875:Ubiquitination
chemical_list: C557465:1-(2-methylpropyl)-1H-imidazo(4,5-c)quinolin 4-amine; D048868:Adaptor Proteins, Signal Transducing; D016207:Cytokines; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D016328:NF-kappa B; D011804:Quinolines; D034741:RNA, Small Interfering; D051192:Receptors, Pattern Recognition; C484406:TANK protein, human; C495348:TLR7 protein, human; D048029:TNF Receptor-Associated Factor 6; D051199:Toll-Like Receptor 7; D051193:Toll-Like Receptors; C515578:MARCHF5 protein, human; C581898:Marchf5 protein, mouse; D044767:Ubiquitin-Protein Ligases