title: Kinetics of UV(254) inactivation of selected viral pathogens in a static system.
abstract: OBJECTIVE
  The objective of this study was to estimate UV(254) inactivation constants for four viral pathogens: influenza virus type A, porcine respiratory and reproductive syndrome virus (PRRSV), bovine viral diarrhoea virus (BVDV) and reovirus.
  
  
  RESULTS
  Viruses in culture medium were exposed to one of nine doses of UV(254) and then titrated for infectious virus. Analysis showed that viral inactivation by UV(254) was more accurately described by a two-stage inactivation model vs a standard one-stage inactivation model.
  
  
  CONCLUSIONS
  The results provided evidence for the existence of two heterogeneous viral subpopulations among the viruses tested, one highly susceptible to UV(254) inactivation and the other more resistant. Importantly, inactivation constants based on the one-stage inactivation model would have underestimated the UV(254) dose required for the inactivation of these viruses under the conditions of the experiment.
  
  
  CONCLUSIONS
  To improve the accuracy of estimates, it is recommended that research involving the inactivation of micro-organisms evaluates inactivation kinetics using both one-stage and two-stage models. These results will be of interest to persons responsible for microbial agents under laboratory or field conditions.
authors.affiliation: Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, IA, USA.
authors.forename: T
authors.initials: T
authors.lastname: Cutler
authors.forename: C
authors.initials: C
authors.lastname: Wang
authors.forename: Q
authors.initials: Q
authors.lastname: Qin
authors.forename: F
authors.initials: F
authors.lastname: Zhou
authors.forename: K
authors.initials: K
authors.lastname: Warren
authors.forename: K-J
authors.initials: KJ
authors.lastname: Yoon
authors.forename: S J
authors.initials: SJ
authors.lastname: Hoff
authors.forename: J
authors.initials: J
authors.lastname: Ridpath
authors.forename: J
authors.initials: J
authors.lastname: Zimmerman
mesh_terms: D000818:Animals; D002460:Cell Line; D003470:Culture Media; D030242:Diarrhea Virus 2, Bovine Viral / Q000528:radiation effects*; D009980:Influenza A virus / Q000528:radiation effects*; D015233:Models, Statistical; D019316:Porcine respiratory and reproductive syndrome virus / Q000528:radiation effects*; D012087:Reoviridae / Q000528:radiation effects*; D014466:Ultraviolet Rays; D010948:Viral Plaque Assay; D038301:Virus Inactivation
chemical_list: D003470:Culture Media