title: Plk1-mediated phosphorylation of UAP56 regulates the stability of UAP56.
abstract: Polo-like kinase 1 (Plk1) is a conserved serine/threonine protein kinase that plays pivotal roles during the cell cycle and cell proliferation. Although a number of important targets have been identified, the mechanism of Plk1-regulated pathways and the bulk of the Plk1 interactome are largely unknown. Here, we demonstrate that Plk1 interacts with the DExH/D RNA helicase, UAP56. The protein levels of UAP56 and Plk1 are inversely correlated during the cell cycle. We also show that Plk1 phosphorylates UAP56 in vitro and in vivo and that Plk1-dependent phosphorylation of UAP56 triggers ubiquitination and degradation of UAP56 through proteasomes. This result suggests that Plk1-mediated phosphorylation of UAP56 regulates the stability of UAP56. Our results will be helpful in further understanding mRNA metabolism, cell cycle progression, and the link between mRNA metabolism and cellular function.
authors.affiliation: Beijing Institute of Biotechnology, Beijing 100071, People's Republic of China.
authors.forename: Fuyin
authors.initials: F
authors.lastname: Xiong
authors.forename: Yanli
authors.initials: Y
authors.lastname: Lin
authors.forename: Zhengbin
authors.initials: Z
authors.lastname: Han
authors.forename: Gengshou
authors.initials: G
authors.lastname: Shi
authors.forename: Liyuan
authors.initials: L
authors.lastname: Tian
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Wu
authors.forename: Qiangcheng
authors.initials: Q
authors.lastname: Zeng
authors.forename: Yanrong
authors.initials: Y
authors.lastname: Zhou
authors.forename: Jixian
authors.initials: J
authors.lastname: Deng
authors.forename: Hongxing
authors.initials: H
authors.lastname: Chen
mesh_terms: D000818:Animals; D018797:Cell Cycle Proteins / Q000378:metabolism*; D045744:Cell Line, Tumor; D053487:DEAD-box RNA Helicases / Q000378:metabolism*; D005434:Flow Cytometry; D057809:HEK293 Cells; D006801:Humans; D047468:Immunoprecipitation; D051379:Mice; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D055550:Protein Stability; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics; D014162:Transfection; D020798:Two-Hybrid System Techniques; D000097869:Polo-Like Kinase 1
chemical_list: D018797:Cell Cycle Proteins; D053487:DEAD-box RNA Helicases; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins; D034741:RNA, Small Interfering; D000097869:Polo-Like Kinase 1; C518885:Ddx39b protein, mouse