title: Urban PM2.5 induces ROS generation and RBC damage in COPD patients.
abstract: Particulate matters (PM) produce adverse effects on the respiratory system and cause COPD. These effects are thought to involve intrinsic generation of ROS which are present in ambient PM (transition metals and aromatic organic compounds). Here, we examined the chemical composition and ultra-microscopic structure of PM2.5. The effect of this PM was studied in red blood cell (RBC) membranes (ghosts) from healthy volunteers (n = 11) and COPD patients (n = 43). These effects were compared with that produced by a Fenton metal-catalytic ROS generator. Oxidative biomarkers and cell damage were singificantly increased in presence of PM2.5 or ROS generator in RBC of COPD patients as compared with those in cells from healthy volunteers. In contrast, total SH groups, band 3 phospho-tyrosine phosphatase (PTPase) and glucose-6 phosphate dehydrogenase (G6PD) activities were all diminished in cells from COPD patients. In conclusion, PM2.5 increases damage to RBCs from COPD patients, decreases the activity of PTPase and G6PD, and alters the function of the anionic exchanger (AE1) and the antioxidant response by decreasing SH groups.
authors.affiliation: Departamento de Bioquimica y Biologia Molecular, Instituto Nacional de Perinatologia, Isidro Espinosa de los Reyes (INPerIER), Mexico.
authors.forename: Yessica D
authors.initials: YD
authors.lastname: Torres-Ramos
authors.forename: Araceli
authors.initials: A
authors.lastname: Montoya-Estrada
authors.forename: Alberto M
authors.initials: AM
authors.lastname: Guzman-Grenfell
authors.forename: Javier
authors.initials: J
authors.lastname: Mancilla-Ramirez
authors.forename: Beatriz
authors.initials: B
authors.lastname: Cardenas-Gonzalez
authors.forename: Salvador
authors.initials: S
authors.lastname: Blanco-Jimenez
authors.forename: Jose D
authors.initials: JD
authors.lastname: Sepulveda-Sanchez
authors.forename: Alejandra
authors.initials: A
authors.lastname: Ramirez-Venegas
authors.forename: Juan J
authors.initials: JJ
authors.lastname: Hicks
mesh_terms: D000368:Aged; D000393:Air Pollutants / Q000633:toxicity*; D004912:Erythrocytes / Q000187:drug effects*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010316:Particle Size; D029424:Pulmonary Disease, Chronic Obstructive / Q000097:blood / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D014504:Urban Health
chemical_list: D000393:Air Pollutants; D017382:Reactive Oxygen Species