title: Phylogenetic analysis of serine proteases from Russell's viper (Daboia russelli siamensis) and Agkistrodon piscivorus leucostoma venom.
abstract: Serine proteases are widely found in snake venoms. They have variety of functions including contributions to hemostasis. In this study, five serine proteases were cloned and characterized from two different cDNA libraries: factor V activator (RVV-V), alpha fibrinogenase (RVAF) and beta fibrinogenase (RVBF) from Russell's viper (Daboia russelli siamensis), and plasminogen activator (APL-PA) and protein C activator (APL-C) from Agkistrodon piscivorus leucostoma. The snake venom serine proteases were clustered in phylogenetic tree according to their functions. K(A)/K(S) values suggested that accelerated evolution has occurred in the mature protein coding regions in cDNAs of snake venom serine proteases.
authors.affiliation: Snake Bite and Venom Research Unit, Chula Medical Research Center, Faculty of Medicine, Chulalongkorn University, Rama IV Road, Patumwan District, Bangkok 10330, Thailand.
authors.forename: Pattadon
authors.initials: P
authors.lastname: Sukkapan
authors.forename: Ying
authors.initials: Y
authors.lastname: Jia
authors.forename: Issarang
authors.initials: I
authors.lastname: Nuchprayoon
authors.forename: John C
authors.initials: JC
authors.lastname: Pérez
mesh_terms: D020413:3' Untranslated Regions; D017836:Agkistrodon / Q000378:metabolism*; D000465:Algorithms; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003435:Crotalid Venoms / Q000201:enzymology*; D030561:Databases, Nucleic Acid; D015723:Gene Library; D008969:Molecular Sequence Data; D010802:Phylogeny; D030162:Reptilian Proteins / Q000737:chemistry / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017840:Daboia / Q000378:metabolism*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012697:Serine Endopeptidases / Q000737:chemistry / Q000235:genetics*; D014757:Viper Venoms / Q000201:enzymology*
chemical_list: D020413:3' Untranslated Regions; D003435:Crotalid Venoms; D030162:Reptilian Proteins; D014757:Viper Venoms; D012697:Serine Endopeptidases