title: Characterization of a newly discovered Beauveria bassiana isolate to Franklimiella occidentalis Perganda, a non-native invasive species in China.
abstract: In this study, a new virulent Beauveria bassiana isolate (B. bassiana-CYT5) had been identified as a new member of the species B. bassiana. The B. bassiana-CYT5 isolate was compared with four other virulent B. bassiana isolates and found to be highly infectious and virulent against the Franklimiella occidentalis Perganda. The B. bassiana-CYT5 could approximately 93.08% mortality of F. occidentalis 6 days post inoculation in the concentration of 1×10(8) conidia/mL. The phylogenetic tree based on ITS and partial sequence of elongation factor 1-alpha (EF1-alpha) indicated that B. bassiana-CYT5 isolate was in a cluster of B. bassiana. Furthermore, B. bassiana-CYT5 isolate demonstrated high heat tolerance (60-100% relative germination) between 1-h and 2-h exposure at 37 °C, 38 °C, 39 °C and 40 °C, respectively. So our results suggested that B. bassiana-CYT5 isolate could be a new efficient biocontrol agent against F. occidentalis.
authors.affiliation: College of Agronomy and Plant Protection, Qingdao Agricultural university, Chengyang District, Qingdao 266109, Shandong Province, China.
authors.forename: Junping
authors.initials: J
authors.lastname: Wang
authors.forename: Changying
authors.initials: C
authors.lastname: Zheng
mesh_terms: D000818:Animals; D052982:Beauveria / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification* / Q000472:pathogenicity; D002681:China; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics; D007313:Insecta / Q000382:microbiology*; D058865:Introduced Species; D008969:Molecular Sequence Data; D020648:Peptide Elongation Factor 1 / Q000235:genetics; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D016019:Survival Analysis; D013696:Temperature
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D005656:Fungal Proteins; D020648:Peptide Elongation Factor 1