title: Constructing the metabolic and regulatory pathways in germinating rice seeds through proteomic approach.
abstract: Construction of metabolic and regulatory pathways from proteomic data can contextualize the large-scale data within the overall physiological scheme of an organism. It is an efficient way to predict metabolic phenotype or regulatory style. We did protein profiling in the germinating rice seeds through 1-DE via LC MS/MS proteomic shotgun strategy. In total, 673 proteins were identified, and could be sorted into 14 functional groups. The largest group was metabolism related. The metabolic proteins were integrated into different metabolic pathways to show the style of reserves mobilization and precursor preparation during the germination. Analysis of the regulatory proteins indicated that regulation of redox homeostasis and gene expression also play important roles for the rice seed germination. Although transcription is unnecessary for the germination, it could ensure the rapidity and uniformity of germination. On the contrary, translation with the stored mRNA is required for the germination. This study will help us to further understand the metabolic style, regulation of redox homeostasis, and gene expression during rice seed germination.
authors.affiliation: Key Laboratory of Plant Germplasm Enhancement and Speciality Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, P. R. China.
authors.forename: Dongli
authors.initials: D
authors.lastname: He
authors.forename: Chao
authors.initials: C
authors.lastname: Han
authors.forename: Jialing
authors.initials: J
authors.lastname: Yao
authors.forename: Shihua
authors.initials: S
authors.lastname: Shen
authors.forename: Pingfang
authors.initials: P
authors.lastname: Yang
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D019069:Cell Respiration; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D018506:Gene Expression Regulation, Plant; D018525:Germination / Q000502:physiology; D005943:Gluconeogenesis / Q000502:physiology; D050356:Lipid Metabolism; D053858:Metabolic Networks and Pathways; D012275:Oryza / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010084:Oxidation-Reduction; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012639:Seeds / Q000378:metabolism*; D013213:Starch / Q000378:metabolism
chemical_list: D000596:Amino Acids; D010940:Plant Proteins; D017382:Reactive Oxygen Species; D013213:Starch