title: Single-tube linear DNA amplification (LinDA) for robust ChIP-seq.
abstract: Genome-wide profiling of transcription factors based on massive parallel sequencing of immunoprecipitated chromatin (ChIP-seq) requires nanogram amounts of DNA. Here we describe a high-fidelity, single-tube linear DNA amplification method (LinDA) for ChIP-seq and reChIP-seq with picogram DNA amounts obtained from a few thousand cells. This amplification technology will facilitate global analyses of transcription-factor binding and chromatin with very small cell populations, such as stem or cancer-initiating cells.
authors.affiliation: Department of Cancer Biology, Institute of Genetics and Molecular and Cellular Biology, Strasbourg-Illkirch, France.
authors.forename: Pattabhiraman
authors.initials: P
authors.lastname: Shankaranarayanan
authors.forename: Marco-Antonio
authors.initials: MA
authors.lastname: Mendoza-Parra
authors.forename: Mannu
authors.initials: M
authors.lastname: Walia
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.forename: Luisa M
authors.initials: LM
authors.lastname: Trindade
authors.forename: Hinrich
authors.initials: H
authors.lastname: Gronemeyer
mesh_terms: D000818:Animals; D001483:Base Sequence; D045744:Cell Line, Tumor; D002843:Chromatin / Q000235:genetics / Q000378:metabolism*; D047369:Chromatin Immunoprecipitation; D004247:DNA / Q000032:analysis* / Q000235:genetics; D006801:Humans; D051379:Mice; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods*; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D002843:Chromatin; D014157:Transcription Factors; D004247:DNA