title: Ethanolic extract of propolis promotes reverse cholesterol transport and the expression of ATP-binding cassette transporter A1 and G1 in mice.
abstract: The ethanolic extract of propolis (EEP) is beneficial in increasing high density lipoprotein (HDL) cholesterol (HDL-C) and diminishing risks of atherosclerosis. In this study, we examined the effects of EEP on reverse cholesterol transport in mice. (3)H -cholesterol laden macrophage was injected intraperitoneally into mice fed by gastric gavage with EEP. Plasma lipid level was determined and (3)H-cholesterol was traced in plasma, liver and feces. The effects of EEP on ATP-binding cassette transporter A1 and G1 (ABCA1 and ABCG1) and scavenger receptor BI (SR-BI) in mice liver and in cultured cells were also investigated. EEP administration led to a significant increase in HDL-C and peritoneal macrophage-original (3)H-cholesterol in plasma, liver and feces. Liver protein expressions of ABCA1 and ABCG1 were increased but SR-B1 was not. In vitro experiments with HepG2 and Raw264.7 cell lines confirmed the above results. The finding of these studies shows that EEP-enhanced reverse cholesterol transport may have resulted from EEP stimulated plasma HDL level and hepatic ABCA1 and ABCG1 expression.
authors.affiliation: Institute of Atherosclerosis, Taishan Medical University, 2# Yingsheng E Road, Taian, 271000, Shandong, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yu
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Si
authors.forename: Guohua
authors.initials: G
authors.lastname: Song
authors.forename: Tian
authors.initials: T
authors.lastname: Luo
authors.forename: Jiafu
authors.initials: J
authors.lastname: Wang
authors.forename: Shucun
authors.initials: S
authors.lastname: Qin
mesh_terms: D064286:ATP Binding Cassette Transporter 1; D000070998:ATP Binding Cassette Transporter, Subfamily G, Member 1; D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D000924:Anticholesteremic Agents / Q000494:pharmacology*; D002784:Cholesterol / Q000097:blood*; D000431:Ethanol; D005243:Feces / Q000737:chemistry; D015870:Gene Expression; D056945:Hep G2 Cells; D006801:Humans; D008074:Lipoproteins / Q000235:genetics / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D011429:Propolis / Q000494:pharmacology*; D051122:Scavenger Receptors, Class B / Q000378:metabolism
chemical_list: C577836:ABCA1 protein, human; C487540:ABCG1 protein, mouse; D064286:ATP Binding Cassette Transporter 1; D000070998:ATP Binding Cassette Transporter, Subfamily G, Member 1; D018528:ATP-Binding Cassette Transporters; D000924:Anticholesteremic Agents; D008074:Lipoproteins; C454739:Scarb1 protein, mouse; D051122:Scavenger Receptors, Class B; D000431:Ethanol; D011429:Propolis; D002784:Cholesterol