title: Amino acid substitution at position 95 in rabies virus matrix protein affects viral pathogenicity.
abstract: We previously reported that rabies virus strain CE(NiM), but not the parental Ni-CE strain, killed mice after intracerebral inoculation. CE(NiM) and Ni-CE are genetically identical except for two amino acids at positions 29 and 95 in the M protein. In this study, to identify which residue determines the pathogenicity, we examined pathogenicities of two Ni-CE mutants, CE(NiM29) and CE(NiM95), which were established by replacement of an amino acid residue at position 29 or 95 in the Ni-CE M protein with the corresponding residue of CE(NiM), respectively. We found that CE(NiM95), but not CE(NiM29), killed mice, indicating that the amino acid at position 95 in the M protein is the pathogenic determinant.
authors.affiliation: Laboratory of Zoonotic Diseases, Faculty of Applied Biological Sciences, Gifu University, Gifu, Japan.
authors.forename: Naoto
authors.initials: N
authors.lastname: Ito
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Mita
authors.forename: Kenta
authors.initials: K
authors.lastname: Shimizu
authors.forename: Yuki
authors.initials: Y
authors.lastname: Ito
authors.forename: Tatsunori
authors.initials: T
authors.lastname: Masatani
authors.forename: Keisuke
authors.initials: K
authors.lastname: Nakagawa
authors.forename: Satoko
authors.initials: S
authors.lastname: Yamaoka
authors.forename: Masako
authors.initials: M
authors.lastname: Abe
authors.forename: Kota
authors.initials: K
authors.lastname: Okadera
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Minamoto
authors.forename: Makoto
authors.initials: M
authors.lastname: Sugiyama
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D005260:Female; D051379:Mice; D011818:Rabies / Q000401:mortality / Q000821:virology*; D011820:Rabies virus / Q000235:genetics / Q000472:pathogenicity*; D014763:Viral Matrix Proteins / Q000235:genetics*
chemical_list: D014763:Viral Matrix Proteins