title: Different expression patterns of CEACAM1 and its impacts on angiogenesis in gastric nonneoplastic and neoplastic lesions.
abstract: OBJECTIVE
  This study was designed to investigate the expression patterns of CEACAM1 and its relationship with angiogenesis in nonneoplastic and neoplastic gastric lesions.
  
  
  METHODS
  CEACAM1 and TGF-β expression was detected by immunohistochemical staining and dual-labeling immunohistochemical staining in neoplastic and nonneoplastic lesions. MVD-CD31 and MVD-CD105 were counted in CEACAM1-positive areas by dual-labeling immunohistochemistry.
  
  
  RESULTS
  There was no expression of CEACAM1 in normal gastric mucosa. In IM and GIN, CEACAM1 was mainly expressed with membranous pattern. CEACAM1 was expressed with membranous pattern in well-differentiated adenocarcinoma, with cytoplasmic pattern in poorly differentiated adenocarcinoma, and with cytoplasmic and membranous pattern mixed together in intermediately adenocarcinoma. The expression patterns of CEACAM1 showed a significant difference (P < 0.05) in nonneoplastic and neoplastic lesions. Coexpression of CEACAM1 and TGF-β was elevated and significantly different from nonneoplastic to neoplastic lesions (P < 0.05). Moreover, CEACAM1 and TGF-β coexpression were related to carcinoma progression (r = 0.35; P < 0.05). MVD-CD31 and MVD-CD105 showed significant differences from nonneoplastic to neoplastic lesions (P < 0.05).
  
  
  CONCLUSIONS
  CEACAM1 has different expression patterns in nonneoplastic and neoplastic lesions. The coexpression of CEACAM1 and TGF-β increased from nonneoplastic to neoplastic lesions and may be related with tumor progression via promoting tumorous angiogenesis.
authors.affiliation: Department of Digestive Disease, The Second Hospital, Shandong University, Jinan, Shandong, People's Republic of China.
authors.forename: Jian-Qiang
authors.initials: JQ
authors.lastname: Guo
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Yu
authors.forename: Hong-Juan
authors.initials: HJ
authors.lastname: Wang
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.forename: Kong-Xi
authors.initials: KX
authors.lastname: Zhu
authors.forename: Qing-Hui
authors.initials: QH
authors.lastname: Zhang
authors.forename: Ting-Guo
authors.initials: TG
authors.lastname: Zhang
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Xu
authors.forename: Hong-Bo
authors.initials: HB
authors.lastname: Wang
authors.forename: Hong-Li
authors.initials: HL
authors.lastname: Wu
authors.forename: Cheng-Jun
authors.initials: CJ
authors.lastname: Zhou
mesh_terms: D000704:Analysis of Variance; D015703:Antigens, CD / Q000378:metabolism*; D002278:Carcinoma in Situ / Q000098:blood supply / Q000378:metabolism* / Q000473:pathology*; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D016009:Chi-Square Distribution; D000071063:Endoglin; D005753:Gastric Mucosa / Q000098:blood supply / Q000378:metabolism; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000098:blood supply / Q000473:pathology*; D008679:Metaplasia / Q000378:metabolism; D055806:Microvessels / Q000378:metabolism; D009367:Neoplasm Staging; D009389:Neovascularization, Pathologic / Q000378:metabolism*; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism; D013274:Stomach Neoplasms / Q000098:blood supply / Q000378:metabolism* / Q000473:pathology*; D016212:Transforming Growth Factor beta / Q000378:metabolism
chemical_list: D015703:Antigens, CD; C069847:CD66 antigens; D015815:Cell Adhesion Molecules; C064206:ENG protein, human; D000071063:Endoglin; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D011956:Receptors, Cell Surface; D016212:Transforming Growth Factor beta