title: Evaluation of early-stage osteochondral defect repair using a biphasic scaffold based on a collagen-glycosaminoglycan biopolymer in a caprine model.
abstract: The aim of this study was to evaluate a new collagen-GAG-calcium phosphate biphasic scaffold for the repair of surgically created osteochondral defects in goats. Comparison of morphological, histological and mechanical performance of the repair tissue was made with defects repaired using a synthetic polymer scaffold. Defects were created in the medial femoral condyle (MFC) and lateral trochlear sulcus (LTS) of Boer Cross goats and evaluated at 12 and 26 weeks. It was found that the total histology score of the collagen-GAG based biomaterial (23.8; SD 1.7) provided a significant improvement (p<0.05) over the biphasic PLGA material (19;3) and the empty control defect (17.3;1.2) in the LTS. The overall trajectory of histological and morphological improvement between 12 and 26 weeks was found to be higher for the collagen-GAG scaffold compared to the PLGA material. The occurrence of sub-chondral bone cysts was lower for the collagen-GAG scaffold with an incidence of 17% of defects, compared to 67% for the PLGA material at 26 weeks. The cartilage repair tissue for both materials evaluated was superior after 26 weeks implantation than the empty control with 75% of the collagen-GAG-treated defects showing markedly more hyaline-like cartilage and 50% of the PLGA sites exhibiting hyaline-like appearances, compared to 17% for the empty control. These early stage data indicate biphasic scaffolds based on collagen-GAG and PLGA both provide indications of satisfactory development of a structural repair to surgically prepared osteochondral defects. Furthermore, the biomaterial composition of the collagen-GAG may provide a more favourable environment for osteochondral repair.
authors.affiliation: The University of Cambridge Orthopaedic Research Unit, Box 180, Addenbrooke's Hospital, Hills Road, Cambridge, CB2 2QQ, UK. agetgood@btinternet.com
authors.forename: Alan M J
authors.initials: AM
authors.lastname: Getgood
authors.forename: Simon J
authors.initials: SJ
authors.lastname: Kew
authors.forename: Roger
authors.initials: R
authors.lastname: Brooks
authors.forename: Harold
authors.initials: H
authors.lastname: Aberman
authors.forename: Timothy
authors.initials: T
authors.lastname: Simon
authors.forename: Andrew K
authors.initials: AK
authors.lastname: Lynn
authors.forename: Neil
authors.initials: N
authors.lastname: Rushton
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000473:pathology*; D003094:Collagen; D006025:Glycosaminoglycans; D006041:Goats; D008297:Male; D008422:Materials Testing; D011108:Polymers; D054457:Tissue Scaffolds
chemical_list: D006025:Glycosaminoglycans; D011108:Polymers; C058506:collagen-glycosaminoglycan copolymer; D003094:Collagen