title: Rapid monitoring of mono- and disaccharides in drinks, foodstuffs and foodstuff additives by capillary electrophoresis with contactless conductivity detection.
abstract: A capillary electrophoresis (CE) procedure with contactless conductivity detection (C(4)D) has been developed for monitoring of neutral mono- and disaccharides in drinks and foodstuffs. The separation of a mixture of seven neutral saccharides (glucose, fructose, galactose, mannose, ribose, sucrose and lactose) employed a quartz capillary, 5 μm i.d., with an effective length of 18.3 cm, and 75 mM NaOH (pH 12.8) as the background electrolyte (BGE). The limit of detection (LOD) values obtained lied within a range from 0.4 μmol L(-1) for lactose to 0.9 μmol L(-1) for ribose, with a separation time shorter than 140 s. The procedure was successfully applied to determinations of saccharides in fruit juices, Coca-Cola, milk, red and white wines, yoghurts, honey and a foodstuff additive.
authors.affiliation: Institute of Biochemistry, Cell and Molecular Biology, Third Faculty of Medicine, Charles University, Prague, Czech Republic. petr.tuma@lf3.cuni.cz
authors.forename: Petr
authors.initials: P
authors.lastname: Tůma
authors.forename: Klára
authors.initials: K
authors.lastname: Málková
authors.forename: Eva
authors.initials: E
authors.lastname: Samcová
authors.forename: Karel
authors.initials: K
authors.lastname: Stulík
mesh_terms: D000818:Animals; D001628:Beverages / Q000032:analysis*; D004187:Disaccharides / Q000032:analysis*; D004553:Electric Conductivity; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D005503:Food Additives / Q000032:analysis*; D005504:Food Analysis / Q000295:instrumentation / Q000379:methods*; D057230:Limit of Detection; D009005:Monosaccharides / Q000032:analysis*; D012266:Ribose / Q000032:analysis; D013997:Time Factors
chemical_list: D004187:Disaccharides; D005503:Food Additives; D009005:Monosaccharides; D012266:Ribose