title: Label-free detection of HIV-2 antibodies in serum with an ultra-high frequency acoustic wave sensor.
abstract: Herein is described a label-free immunosensor dedicated to the detection of HIV-2. The biosensor platform is constructed as a mixed self-assembled monolayer-coated quartz wafer onto which HIV-2 immunodominant epitopes are immobilized. The biosensing properties, in terms of specific vs. non-specific antigen-antibody interactions, are evaluated with the electromagnetic piezoelectric acoustic sensor (EMPAS) using equimolar serum solutions of HIV-2 or HIV-1 monoclonal antibodies, respectively. This immunosensor constitutes the first real-world application of the EMPAS technology in the bioanalytical field.
authors.affiliation: Department of Chemistry, University of Toronto, 80 St. George Street, Toronto, ON, Canada M5S 3H6.
authors.forename: Sonia
authors.initials: S
authors.lastname: Sheikh
authors.forename: Christophe
authors.initials: C
authors.lastname: Blaszykowski
authors.forename: Michael
authors.initials: M
authors.lastname: Thompson
mesh_terms: D000162:Acoustics / Q000295:instrumentation; D000911:Antibodies, Monoclonal; D015374:Biosensing Techniques / Q000379:methods*; D015483:HIV Antibodies / Q000097:blood* / Q000276:immunology; D015497:HIV-1 / Q000276:immunology; D015498:HIV-2 / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D055767:Immobilized Proteins; D016056:Immunodominant Epitopes / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D015483:HIV Antibodies; D055767:Immobilized Proteins; D016056:Immunodominant Epitopes