title: The styryl dye FM1-43 suppresses odorant responses in a subset of olfactory neurons by blocking cyclic nucleotide-gated (CNG) channels.
abstract: Many olfactory receptor neurons use a cAMP-dependent transduction mechanism to transduce odorants into depolarizations. This signaling cascade is characterized by a sequence of two currents: a cation current through cyclic nucleotide-gated channels followed by a chloride current through calcium-activated chloride channels. To date, it is not possible to interfere with these generator channels under physiological conditions with potent and specific blockers. In this study we identified the styryl dye FM1-43 as a potent blocker of native olfactory cyclic nucleotide-gated channels. Furthermore, we characterized this substance to stain olfactory receptor neurons that are endowed with cAMP-dependent transduction. This allows optical differentiation and pharmacological interference with olfactory receptor neurons at the level of the signal transduction.
authors.affiliation: Department of Neurophysiology and Cellular Biophysics, University of Göttingen,37073 Göttingen, Germany.
authors.forename: Esther
authors.initials: E
authors.lastname: Breunig
authors.forename: Eugen
authors.initials: E
authors.lastname: Kludt
authors.forename: Dirk
authors.initials: D
authors.lastname: Czesnik
authors.forename: Detlev
authors.initials: D
authors.lastname: Schild
mesh_terms: D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000494:pharmacology*; D007473:Ion Channels / Q000037:antagonists & inhibitors* / Q000378:metabolism; D007814:Larva / Q000166:cytology / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D009833:Olfactory Pathways / Q000166:cytology / Q000378:metabolism*; D011726:Pyridinium Compounds / Q000494:pharmacology*; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology*; D009435:Synaptic Transmission / Q000187:drug effects*; D014982:Xenopus laevis
chemical_list: C073804:FM1 43; D005456:Fluorescent Dyes; D007473:Ion Channels; D011726:Pyridinium Compounds; D000644:Quaternary Ammonium Compounds; D000242:Cyclic AMP