title: Metabolism of free oligosaccharides is facilitated in the och1Δ mutant of Saccharomyces cerevisiae.
abstract: In eukaryotic cells, it is known that N-glycans play a pivotal role in quality control of carrier proteins. Although "free" forms of oligosaccharides (fOSs) are known to be accumulated in the cytosol, the precise mechanism of their formation, degradation and biological relevance remains poorly understood. It has been shown that, in budding yeast, almost all fOSs are formed from misfolded glycoproteins. Precise structural analysis of fOSs revealed that several yeast fOSs bear a yeast-specific modification by Golgi-resident α-1,6-mannosyltransferase, Och1. In this study, structural diversity of fOSs in och1Δ cells was analyzed. To our surprise, several fOSs in och1Δ cells have unusual α-1,3-linked mannose residues at their non-reducing termini. These mannose residues were not observed in wild-type cells, suggesting that the addition of these unique mannoses occurred as a compensation of Och1 defect. A significant increase in the amount of fOSs modified by Golgi-localized mannosyltransferases was also observed in och1Δ cells. Moreover, the amount of processed fOSs and intracellular α-mannosidase (Ams1) both increased in this mutant. Up-regulation of Ams1 activity was also apparent for cells treated with cell wall perturbation reagent. These results provide an insight into a possible link between catabolism of fOSs and cell wall stress.
authors.affiliation: Glycometabolome Team, Systems Glycobiology Research Group, RIKEN Advanced Science Institute, 2-1 Hirosawa, Wako Saitama 351-0198, Japan.
authors.forename: Hiroto
authors.initials: H
authors.lastname: Hirayama
authors.forename: Tadashi
authors.initials: T
authors.lastname: Suzuki
mesh_terms: D006056:Golgi Apparatus / Q000378:metabolism; D008358:Mannose / Q000737:chemistry / Q000378:metabolism; D008364:Mannosyltransferases / Q000235:genetics / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation; D043323:alpha-Mannosidase / Q000235:genetics / Q000378:metabolism
chemical_list: D008562:Membrane Glycoproteins; C075636:OCH1 protein, S cerevisiae; D009844:Oligosaccharides; D029701:Saccharomyces cerevisiae Proteins; D008364:Mannosyltransferases; D043323:alpha-Mannosidase; D008358:Mannose