title: The use of multidimensional liquid-phase separations and mass spectrometry for the detailed characterization of posttranslational modifications in glycoproteins.
abstract: The goal of characterization of the proteome, while challenging in itself, is further complicated by the microheterogeneity introduced by posttranslational modifications such as glycosylation. A combination of liquid chromatography (LC), capillary electrophoresis (CE), and mass spectrometry (MS) offers the advantages of unique selectivity and high efficiency of the separation methods combined with the mass specificity and sensitivity of MS. In the current work, the combination of liquid-phase separations and mass spectrometry is demonstrated through the on-line coupling of electrospray ionization mass spectrometry (ESI-MS) and off-line coupling with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF-MS). LC/ESI-MS yields real-time results while maintaining the separation obtained from the LC analysis. CE/MALDI TOF-MS offers high-mass detection and extremely low detection limits. The unique separation selectivity of CE relative to reversed-phase HPLC separations of the members of a glycopeptide family was used to develop an integrated multidimensional analysis achieved by the off-line coupling of LC, CE, and MALDI TOF-MS. To demonstrate the applicability of these techniques to the characterization of the heterogeneity of posttranslational modifications present in glycoproteins, we will report on the study of the glycoforms present in a N-linked site in a single-chain plasminogen activator (DSPAα1).
authors.affiliation: Analytical/Medical Laboratory, Hewlett-Packard Laboratories, 3500 Deer Creek Road, Palo Alto, California 94304, and Berlex Biosciences, Richmond, California 94804.
authors.forename: S
authors.initials: S
authors.lastname: Udiavar
authors.forename: A
authors.initials: A
authors.lastname: Apffel
authors.forename: J
authors.initials: J
authors.lastname: Chakel
authors.forename: S
authors.initials: S
authors.lastname: Swedberg
authors.forename: W S
authors.initials: WS
authors.lastname: Hancock
authors.forename: E
authors.initials: E
authors.lastname: Pungor