title: Sensitive luciferin derived probes for selective carboxypeptidase activity.
abstract: Highly selective luminescent probes, QLUC-TYR and LUC-GLU, for detection of carboxypeptidase activity were synthesized. Caged substrates were first cleaved by corresponding carboxypeptidases, and then they were activated by luciferase to emit light. Enzymatic activities of biologically important carboxypeptidases can be determined using this technology.
authors.affiliation: Department of Radiology, The Methodist Hospital Research Institute, Weill Cornell Medical College, 6565 Fannin Street, #B5-009, Houston, TX 77030, United States.
authors.forename: Yu-Cheng
authors.initials: YC
authors.lastname: Chang
authors.forename: Pei-Wen
authors.initials: PW
authors.lastname: Chao
authors.forename: Ching-Hsuan
authors.initials: CH
authors.lastname: Tung
mesh_terms: D002268:Carboxypeptidases / Q000032:analysis* / Q000737:chemistry; D049408:Luminescent Agents / Q000138:chemical synthesis* / Q000737:chemistry; D015394:Molecular Structure; D013379:Substrate Specificity
chemical_list: D049408:Luminescent Agents; D002268:Carboxypeptidases