title: Molecular cloning and expression analysis of a RGA-like gene responsive to plant hormones in Brassica napus.
abstract: DELLA proteins are negative regulators of GA-induced growth. DELLA protein family is characterized by a DELLA domain essential for GA-dependent proteasomal degradation of DELLA repressors. A full-length cDNA encoding a putative DELLA protein with high sequence homology to Arabidopsis thaliana RGA (AtRGA), designated as BnRGA, was isolated from Brassica napus. The full-length cDNA of BnRGA contained a 1,740 bp open reading frame (ORF) encoding a precursor protein of 579 amino acid residues. Comparative and bioinformatics analyses revealed that BnRGA showed a high degree of homology with DELLA proteins and contained the DELLA domain, TVHYNP domain, VHIID domain and RVER domain. Using real-time PCR, the expression patterns of BnRGA and two our previously isolated genes, BnGID1a and BnSLY1 in B. napus, were analyzed by adding exogenous gibberellins acid-3 (GA(3)), GA biosynthetic inhibitor paclobutrazol (PAC) and abscisic acid (ABA). The results showed that the expression of BnGID1a and BnSLY1 was down-regulated after treated by GA(3) and induced by PAC and ABA. These results suggest that the expression of BnGID1a and BnSLY1 may be negatively regulated by the level of endogenous GA in B. napus. Moreover, BnRGA was not significantly regulated by GA(3), PAC and ABA in the low concentrations. These suggest that GA-GID1-SCF-DELLA complex may have a mechanism of self-regulation, thereby preserving the stability of the expression level of BnRGA in B. napus.
authors.affiliation: College of Bioscience and Biotechnology, Key Laboratories of Crop Genetics and Physiology of the Jiangsu Province and Plant Functional Genomics of the Ministry of Education, Yangzhou University, Yangzhou, 225009 Jiangsu, People's Republic of China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Gao
authors.forename: Jianmin
authors.initials: J
authors.lastname: Chen
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhao
authors.forename: Tingting
authors.initials: T
authors.lastname: Li
authors.forename: Maolin
authors.initials: M
authors.lastname: Wang
mesh_terms: D029681:Arabidopsis Proteins / Q000235:genetics; D001483:Base Sequence; D029688:Brassica napus / Q000235:genetics* / Q000254:growth & development; D003001:Cloning, Molecular; D019295:Computational Biology; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D005875:Gibberellins / Q000378:metabolism; D008969:Molecular Sequence Data; D046912:Multiprotein Complexes / Q000235:genetics / Q000378:metabolism*; D016366:Open Reading Frames / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D017434:Protein Structure, Tertiary; D060888:Real-Time Polymerase Chain Reaction; D012097:Repressor Proteins / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D029681:Arabidopsis Proteins; D017931:DNA Primers; D018076:DNA, Complementary; D005875:Gibberellins; D046912:Multiprotein Complexes; D010940:Plant Proteins; C508468:RGA protein, Arabidopsis; D012097:Repressor Proteins