title: [Linkage analysis of susceptibility loci in 2 target chromosomes in pedigrees with paranoid schizophrenia and undifferentiated schizophrenia].
abstract: OBJECTIVE
  To investigate the relationship of susceptibility loci in chromosomes 1q21-25 and 6p21-25 and schizophrenia subtypes in Chinese population.
  
  
  METHODS
  A genomic scan and parametric and non-parametric analyses were performed on 242 individuals from 36 schizophrenia pedigrees, including 19 paranoid schizophrenia and 17 undifferentiated schizophrenia pedigrees, from Henan province of China using 5 microsatellite markers in the chromosome region 1q21-25 and 8 microsatellite markers in the chromosome region 6p21-25, which were the candidates of previous studies. All affected subjects were diagnosed and typed according to the criteria of the Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition, Text Revised (DSM-IV-TR; American Psychiatric Association, 2000). All subjects signed informed consent.
  
  
  RESULTS
  In chromosome 1, parametric analysis under the dominant inheritance mode of all 36 pedigrees showed that the maximum multi-point heterogeneity Log of odds score method (HLOD) score was 1.33 (α = 0.38). The non-parametric analysis and the single point and multi-point nonparametric linkage (NPL) scores suggested linkage at D1S484, D1S2878, and D1S196. In the 19 paranoid schizophrenias pedigrees, linkage was not observed for any of the 5 markers. In the 17 undifferentiated schizophrenia pedigrees, the multi-point NPL score was 1.60 (P= 0.0367) at D1S484. The single point NPL score was 1.95(P= 0.0145) and the multi-point NPL score was 2.39 (P= 0.0041) at D1S2878. Additionally, the multi-point NPL score was 1.74 (P= 0.0255) at D1S196. These same three loci showed suggestive linkage during the integrative analysis of all 36 pedigrees. In chromosome 6, parametric linkage analysis under the dominant and recessive inheritance and the non-parametric linkage analysis of all 36 pedigrees and the 17 undifferentiated schizophrenia pedigrees, linkage was not observed for any of the 8 markers. In the 19 paranoid schizophrenias pedigrees, parametric analysis showed that under recessive inheritance mode the maximum single-point HLOD score was 1.26 (α = 0.40) and the multi-point HLOD was 1.12 (α = 0.38) at D6S289 in the chromosome 6p23. In nonparametric analysis, the single-point NPL score was 1.52 (P= 0.0402) and the multi-point NPL score was 1.92 (P= 0.0206) at D6S289.
  
  
  CONCLUSIONS
  Susceptibility genes correlated with undifferentiated schizophrenia pedigrees from D1S484, D1S2878, D1S196 loci, and those correlated with paranoid schizophrenia pedigrees from D6S289 locus are likely present in chromosome regions 1q23.3 and 1q24.2, and chromosome region 6p23, respectively.
authors.affiliation: The Clinical Laboratory of No. 261 Hospital of the People's Liberation Army, Beijing, 100094 P. R. China.
authors.forename: Li-ping
authors.initials: LP
authors.lastname: Zeng
authors.forename: Zheng-mao
authors.initials: ZM
authors.lastname: Hu
authors.forename: Li-li
authors.initials: LL
authors.lastname: Mu
authors.forename: Gui-sen
authors.initials: GS
authors.lastname: Mei
authors.forename: Xiu-ling
authors.initials: XL
authors.lastname: Lu
authors.forename: Yong-jun
authors.initials: YJ
authors.lastname: Zheng
authors.forename: Pei-jian
authors.initials: PJ
authors.lastname: Li
authors.forename: Ying-xue
authors.initials: YX
authors.lastname: Zhang
authors.forename: Qian
authors.initials: Q
authors.lastname: Pan
authors.forename: Zhi-gao
authors.initials: ZG
authors.lastname: Long
authors.forename: He-ping
authors.initials: HP
authors.lastname: Dai
authors.forename: Zhuo-hua
authors.initials: ZH
authors.lastname: Zhang
authors.forename: Jia-hui
authors.initials: JH
authors.lastname: Xia
authors.forename: Jing-ping
authors.initials: JP
authors.lastname: Zhao
authors.forename: Kun
authors.initials: K
authors.lastname: Xia
mesh_terms: D000328:Adult; D002877:Chromosomes, Human; D008040:Genetic Linkage; D056426:Genetic Loci; D020022:Genetic Predisposition to Disease; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics; D008875:Middle Aged; D012559:Schizophrenia / Q000235:genetics*; D055815:Young Adult