title: [Effect of different anesthesia methods on immune function in patients of laparoscopic cholecystectomy in peri-operational period].
abstract: OBJECTIVE
  To compare the influence of acupuncture combine with general anesthesia (ACGA) and general anesthesia (GA) on immune function in patients of laparoscopic cholecystectomy (LC) in peri-operational period.
  
  
  METHODS
  Thirty-nine cases undergoing LC were randomly divided into an ACGA group and a GA group. The ACGA group was treated with electroacupuncture at Hegu (LI 4), Zusanli (ST 36) and Yanglingquan (GB 34) for 15-30 minutes and subsequently with the general anesthesia followed by continuous electroacupuncture stimulation till the operation finished. The GA group was treated with simple general anesthesia. Changes of T cell subset, tumor necrosis factor-apha (TNF-alpha) and interleukin-6 (IL-6) were observed at time points of before anesthesia induction, 2 hours after operation, 1 and 3 days after operation and the occurrence of adverse reaction after operation was recorded.
  
  
  RESULTS
  The percentages of CD3+ and CD8+ in both groups at 2 hours after operation were significant lower than that before anesthesia induction (all P < 0.05), and the percentage of CD4+ in the GA group decreased significantly at that time point (P < 0.05), while there was no significant difference in the ACGA group and CD4+/CD8+ increased significantly (P < 0.05). The level of TNF-alpha in the ACGA group decreased significantly at 3 days after operation as compared with that of before anesthesia induction (P < 0.05). There were significantly less cases nauseated after operation in the ACGA group than that in the GA group (P < 0.05).
  
  
  CONCLUSIONS
  Acupuncture combine with general anesthesia has very little effect on immune function in patients of LC with less adverse reactions.
authors.affiliation: Department of Anesthesiology, Yueyang Hospital of Integrated Chinese and Western Medicine Affiliated to Shanghai University of TCM, Shanghai 200437, China. danny_z_q@hotmail.com
authors.forename: Chen-Yi
authors.initials: CY
authors.lastname: Gu
authors.forename: Li-Rong
authors.initials: LR
authors.lastname: Shen
authors.forename: Yi-Hong
authors.initials: YH
authors.lastname: Ding
authors.forename: Yi
authors.initials: Y
authors.lastname: Lou
authors.forename: Huan-Gan
authors.initials: HG
authors.lastname: Wu
authors.forename: Zheng
authors.initials: Z
authors.lastname: Shi
authors.forename: Xiao-Peng
authors.initials: XP
authors.lastname: Ma
mesh_terms: D015667:Acupuncture Analgesia; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000758:Anesthesia / Q000379:methods*; D016516:CD4-CD8 Ratio; D017081:Cholecystectomy, Laparoscopic; D005260:Female; D006801:Humans; D007109:Immunity; D008297:Male; D008875:Middle Aged; D059035:Perioperative Period