title: MiR-204 regulates cardiomyocyte autophagy induced by ischemia-reperfusion through LC3-II.
abstract: BACKGROUND
  Autophagy plays a significant role in myocardial ischemia-reperfusion (IR) injury. So it is important to inhibit autophagy to protect cardiomyocytes besides anti-apoptosis. MiRNA has been demonstrated to protect cardiomyocytes against apoptosis during IR, while whether it has anti-autophagy effect has not been known. The aim of this study was to investigate whether miR-204 regulated autophagy by regulating LC3-II protein, which is the marker of autophagosome during myocardial IR injury.
  
  
  METHODS
  Adult SD rats were randomized to Control and IR groups. IR group was treated with 30 min ischemia by ligating the left anterior descending coronary artery, followed by 2 h reperfusion by loosing the ligation. The expression of miR-204 was measured by RT-PCR, and LC3 protein was measured by western-blot.
  
  
  RESULTS
  We found that IR induced cardiomyocytes autophagy, together with down-regulation of miR-204 and up-regulation of LC3-II protein. And, we have found that LC3-II protein was regulated by miR-204, using the method of transferring miR-204 mimic or AMO-204 into the cardiomyocytes, before.
  
  
  CONCLUSIONS
  These studies provided evidence that miR-204 played an important role in regulating autophagy through LC3-IIprotein during IR.
authors.affiliation: Department of Cardiothoracic Surgery, Changzheng Hospital, the Second Military Medical University, Fengyang road #415, Shanghai, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Xiao
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Zhu
authors.forename: Bin
authors.initials: B
authors.lastname: He
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Zhang
authors.forename: Bo
authors.initials: B
authors.lastname: Kang
authors.forename: Zhinong
authors.initials: Z
authors.lastname: Wang
authors.forename: Xin
authors.initials: X
authors.lastname: Ni
mesh_terms: D000818:Animals; D017209:Apoptosis; D001343:Autophagy; D015153:Blotting, Western; D004195:Disease Models, Animal; D015536:Down-Regulation; D006331:Heart Diseases / Q000503:physiopathology; D007511:Ischemia / Q000378:metabolism* / Q000503:physiopathology; D007770:L-Lactate Dehydrogenase / Q000097:blood; D035683:MicroRNAs / Q000378:metabolism*; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D015428:Myocardial Reperfusion Injury / Q000378:metabolism* / Q000503:physiopathology; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000502:physiology; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation
chemical_list: D007770:L-Lactate Dehydrogenase; D035683:MicroRNAs; D008869:Microtubule-Associated Proteins; C526109:Anxa3 protein, rat