title: Xenon fails to inhibit capsaicin-evoked CGRP release by nociceptors in culture.
abstract: To investigate whether the xenon-induced inhibition of the transient receptor potential vanilloid type 1 (TRPV1) ion channel in rat dorsal root ganglion (DRG) neurons reduces nociceptive processing, we examined the effect of xenon in reducing the release of calcitonin gene-related peptide (CGRP) from those neurons. We found that exposure to xenon failed to effect a reduction of capsaicin-evoked CGRP release from cultured primary sensory neurons when stimulated by capsaicin. This finding suggests that xenon acts on several molecular targets on nociceptive primary sensory neurons, and that xenon's action on one, or more, of those targets serves to offset the inhibitory, pro-analgesic, effect of xenon on TRPV1. It is concluded that xenon may not produce any analgesic effect through peripheral nociceptors.
authors.affiliation: Section of Anaesthetics, Pain Medicine and Intensive Care, Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, Chelsea and Westminster Hospital, 369, Fulham Road, London SW10 9NH, United Kingdom.
authors.forename: Guy
authors.initials: G
authors.lastname: Calcott
authors.forename: John P M
authors.initials: JP
authors.lastname: White
authors.forename: Istvan
authors.initials: I
authors.lastname: Nagy
mesh_terms: D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000037:antagonists & inhibitors / Q000378:metabolism*; D002211:Capsaicin / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D002478:Cells, Cultured; D005260:Female; D009619:Nociceptors / Q000378:metabolism* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D050916:TRPV Cation Channels / Q000037:antagonists & inhibitors / Q000378:metabolism*; D014978:Xenon / Q000494:pharmacology*
chemical_list: D050916:TRPV Cation Channels; C494482:TRPV1 protein, human; D014978:Xenon; D015740:Calcitonin Gene-Related Peptide; D002211:Capsaicin