title: Reprogramming of EBV-immortalized B-lymphocyte cell lines into induced pluripotent stem cells.
abstract: EBV-immortalized B lymphocyte cell lines have been widely banked for studying a variety of diseases, including rare genetic disorders. These cell lines represent an important resource for disease modeling with the induced pluripotent stem cell (iPSC) technology. Here we report the generation of iPSCs from EBV-immortalized B-cell lines derived from multiple inherited disease patients via a nonviral method. The reprogramming method for the EBV cell lines involves a distinct protocol compared with that of patient fibroblasts. The B-cell line-derived iPSCs expressed pluripotency markers, retained the inherited mutation and the parental V(D)J rearrangement profile, and differentiated into all 3 germ layer cell types. There was no integration of the reprogramming-related transgenes or the EBV-associated genes in these iPSCs. The ability to reprogram the widely banked patient B-cell lines will offer an unprecedented opportunity to generate human disease models and provide novel drug therapies.
authors.affiliation: Stem Cell Biology Laboratory, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA.
authors.forename: Su Mi
authors.initials: SM
authors.lastname: Choi
authors.forename: Hua
authors.initials: H
authors.lastname: Liu
authors.forename: Pooja
authors.initials: P
authors.lastname: Chaudhari
authors.forename: Yonghak
authors.initials: Y
authors.lastname: Kim
authors.forename: Linzhao
authors.initials: L
authors.lastname: Cheng
authors.forename: Jian
authors.initials: J
authors.lastname: Feng
authors.forename: Saul
authors.initials: S
authors.lastname: Sharkis
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Ye
authors.forename: Yoon-Young
authors.initials: YY
authors.lastname: Jang
mesh_terms: D001402:B-Lymphocytes / Q000166:cytology* / Q000378:metabolism / Q000821:virology*; D002454:Cell Differentiation; D002460:Cell Line; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D065150:Cellular Reprogramming; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000502:physiology*; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D019076:Transgenes