title: Interferon alpha on expression of hTERT mRNA in peripheral blood mononuclear cells of patients with chronic hepatitis B.
abstract: Cell division is closely related to telomerase activity (hTERT mRNA). Lower expression of lymphocitic hTERT mRNA may easily cause cell aging, which is not beneficial to maintaining a durable lymphocyte division. To date, there is no study to investigate IFNα therapy on hTERT mRNA expression in PBMCs of patients with chronic hepatitis B (CHB). We quantitatively detected hTERT mRNA from study subjects and made each hTERT mRNA normalized (NhTERT mRNA). Mean NhTERT mRNA level was lower in either CHB group, but it significantly increased in IFNα-treated group compared with CHB control group, and a longer duration of IFNα therapy could increase the level. Moreover, the mean NhTERT mRNA in subgroup with HBeAg loss was significantly higher than that in subgroup without. NhTERT mRNA was markedly correlated with CD3(+) T lymphocyte count and CD4(+)/CD8(+) ratio. The results showed that IFNα therapy could upregulate the expression of hTERT mRNA in PBMCs.
authors.affiliation: Department of Hepatology, The Fifth People's Hospital of Suzhou, Suzhou 215007, China. zhuchw@126.com
authors.forename: Chuan-wu
authors.initials: CW
authors.lastname: Zhu
authors.forename: Ming
authors.initials: M
authors.lastname: Chen
authors.forename: Xiang-rong
authors.initials: XR
authors.lastname: Luo
authors.forename: Hai-yan
authors.initials: HY
authors.lastname: Wang
authors.forename: Li-hua
authors.initials: LH
authors.lastname: Wang
authors.forename: Jian-hong
authors.initials: JH
authors.lastname: Wu
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.forename: Xue-hua
authors.initials: XH
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Zhu
authors.forename: Jian-zhong
authors.initials: JZ
authors.lastname: Ye
authors.forename: Feng
authors.initials: F
authors.lastname: Qian
mesh_terms: D000328:Adult; D005260:Female; D019694:Hepatitis B, Chronic / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007155:Immunologic Factors / Q000494:pharmacology*; D016898:Interferon-alpha / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D019098:Telomerase / Q000235:genetics* / Q000378:metabolism
chemical_list: D007155:Immunologic Factors; D016898:Interferon-alpha; D012333:RNA, Messenger; C509186:TERT protein, human; D019098:Telomerase