title: Glycosyl part identified within Balanites aegyptiaca fruit protease.
abstract: The many milk-clotting proteases from plant are glycosylated; attachment of monosaccharides to enzyme is an advantage for its activity and stability. In this study, gas chromatography coupled to mass spectrometry-electrospray ionization was used to identify glycans bond to proteases purified from Balanites aegyptiaca fruits pulp through cation exchange chromatography. Carbohydrates were identified according to the retention time and the ion at m/z after derivation by heptafluorobutyric acid. The chromatograms obtained from monosaccharides analysis revealed the presence of galactose, mannose, arabinose, xylose, rhamnose and glucuronic acid. The mass spectrometry-electrospray ionization spectra corroborated these findings.
authors.affiliation: Laboratoire de Biophysique et de Biochimie Alimentaire et Nutrition, Université de Ngaoundéré, BP 455-ENSAI-Ngaoundéré, Cameroun. bekarobertger2004@yahoo.fr
authors.forename: R G
authors.initials: RG
authors.lastname: Beka
authors.forename: V D
authors.initials: VD
authors.lastname: Guiama
authors.forename: A
authors.initials: A
authors.lastname: Delmont
authors.forename: P
authors.initials: P
authors.lastname: Donn
authors.forename: M-C
authors.initials: MC
authors.lastname: Slomianny
authors.forename: D G
authors.initials: DG
authors.lastname: Libouga
authors.forename: C M
authors.initials: CM
authors.lastname: Mbofung
authors.forename: D
authors.initials: D
authors.lastname: Guillochon
authors.forename: D
authors.initials: D
authors.lastname: Vercaigne-Marko
mesh_terms: D032430:Balanites / Q000201:enzymology*; D005638:Fruit / Q000201:enzymology*; D006031:Glycosylation; D009005:Monosaccharides / Q000032:analysis / Q000378:metabolism; D010447:Peptide Hydrolases / Q000302:isolation & purification / Q000378:metabolism*; D014574:Uronic Acids / Q000032:analysis / Q000378:metabolism
chemical_list: D009005:Monosaccharides; D014574:Uronic Acids; D010447:Peptide Hydrolases