title: Evaluation of madurahydroxylactone as a slow release antibacterial implant coating.
abstract: Madurahydroxylactone (MHL), a secondary metabolite with antibacterial activity was evaluated for its suitability to generate controlled drug release coatings on medical implant materials. A smooth and firmly attached layer could be produced from a precursor solution on various metallic implant materials. In physiological salt solutions these coatings dissolved within a time period up to one week. A combination of MHL with a broad spectrum fluoroquinolone antibiotic was used to create a coating that was active against all bacterial strains tested. The time period during which the coating remained active against Pseudomonas aeruginosa was investigated. The results indicated a delayed drug release from single layer coatings in the course of seven days. MHL was biocompatible in cell culture assays and could after a delay even serve as a cell adhesion substrate for human or murine cells. The findings indicate a potential for MHL for the generation of delayed release antimicrobial implant coatings.
authors.affiliation: Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 38124 Braunschweig, Germany.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Badar
authors.forename: Katherina
authors.initials: K
authors.lastname: Hemmen
authors.forename: Manfred
authors.initials: M
authors.lastname: Nimtz
authors.forename: Martin
authors.initials: M
authors.lastname: Stieve
authors.forename: Meike
authors.initials: M
authors.lastname: Stiesch
authors.forename: Thomas
authors.initials: T
authors.lastname: Lenarz
authors.forename: Hansjörg
authors.initials: H
authors.lastname: Hauser
authors.forename: Ute
authors.initials: U
authors.lastname: Möllmann
authors.forename: Sebastian
authors.initials: S
authors.lastname: Vogt
authors.forename: Matthias
authors.initials: M
authors.lastname: Schnabelrauch
authors.forename: Peter P
authors.initials: PP
authors.lastname: Mueller