title: Lowering the UV Absorbance Detection Limit in Capillary Zone Electrophoresis Using a Single Linear Photodiode Array Detector.
abstract: A new approach for lowering the UV absorbance detection limit in capillary electrophoresis is presented. This approach involves the use of a photodiode array in which each of the diodes in the array is treated as an independent detector. Over the course of a run, therefore, an electropherogram is generated for each diode in the array. Averaging the electropherograms generated from 1500 diodes in a diode array resulted in a signal-to-noise ratio 85 times that of an electropherogram generated from any one diode in the array. These signal-to-noise improvements are discussed, and the detection limits are compared to the detection limits obtained from a commercial single-point detector. The array detector improves the detection limit by a factor of 3.8 (±0.4).
authors.affiliation: Kenan Laboratories of Chemistry, Department of Chemistry, The University of North Carolina, Chapel Hill, North Carolina 27599-3290.
authors.forename: C T
authors.initials: CT
authors.lastname: Culbertson
authors.forename: J W
authors.initials: JW
authors.lastname: Jorgenson