title: Progesterone-induced blocking factor (PIBF) and trophoblast invasiveness.
abstract: Controlled trophoblast invasion is a key process during human placentation and a prerequisite for successful pregnancy. Progesterone is one of the factors to regulate trophoblast invasiveness. Progesterone-induced blocking factor (PIBF) is a progesterone-induced molecule expressed by the trophoblast, and also by tumors. The distribution of PIBF within the first-trimester decidua coincides with sites of trophoblast invasion. Another molecule that has been implicated in the control of trophoblast invasiveness is placental leptin. Leptin inhibits the secretion of progesterone by cytotrophoblast. The aim of this work was to investigate the possible interaction of PIBF and leptins in regulating trophoblast invasion. Paraffin-embedded sections from normal first-trimester placentae, partial moles, complete moles, and choriocarcinomas were reacted with PIBF, leptin, and leptin receptor specific antibodies. PIBF-deficient trophoblast cells were generated using siRNA and leptin receptor was detected on Western blot analysis. The lysates of PIBF-treated cells were used for detecting leptin expression in a protein array. PIBF was expressed in both normal first-trimester villous trophoblast and in partial mole. Compared with this, PIBF expression was markedly decreased in complete mole and absent in choriocarcinoma. Neither leptinR nor leptin were detected in partial mole, whereas both of these molecules were present in complete mole and choriocarcinoma. Leptin receptor expression was upregulated in PIBF-deficient cells, while leptin expression was decreased in PIBF-treated cells. These data suggest that PIBF affects the expression of leptin and its receptor, and that PIBF expression is inversely related to trophoblast invasiveness.
authors.affiliation: Department of Medical Microbiology and Immunology, Medical School, Pecs University, 12 Szigeti Street, H-7624 Pecs, Hungary.
authors.forename: E
authors.initials: E
authors.lastname: Miko
authors.forename: M
authors.initials: M
authors.lastname: Halasz
authors.forename: B
authors.initials: B
authors.lastname: Jericevic-Mulac
authors.forename: L
authors.initials: L
authors.lastname: Wicherek
authors.forename: P
authors.initials: P
authors.lastname: Arck
authors.forename: G
authors.initials: G
authors.lastname: Arató
authors.forename: J
authors.initials: J
authors.lastname: Skret Magierlo
authors.forename: D
authors.initials: D
authors.lastname: Rukavina
authors.forename: J
authors.initials: J
authors.lastname: Szekeres-Bartho
mesh_terms: D015153:Blotting, Western; D002460:Cell Line; D002822:Choriocarcinoma / Q000378:metabolism / Q000473:pathology; D003656:Decidua / Q000378:metabolism / Q000473:pathology; D010064:Embryo Implantation / Q000502:physiology; D005260:Female; D006801:Humans; D006828:Hydatidiform Mole / Q000378:metabolism / Q000473:pathology; D020738:Leptin / Q000096:biosynthesis / Q000378:metabolism; D010920:Placenta / Q000378:metabolism / Q000473:pathology; D010929:Placentation / Q000502:physiology; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000235:genetics / Q000378:metabolism*; D011261:Pregnancy Trimester, First; D011374:Progesterone / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering; D054411:Receptors, Leptin / Q000096:biosynthesis / Q000276:immunology; D013491:Suppressor Factors, Immunologic / Q000235:genetics / Q000378:metabolism*; D014327:Trophoblasts / Q000378:metabolism*; D014594:Uterine Neoplasms / Q000378:metabolism / Q000473:pathology
chemical_list: D020738:Leptin; C110058:PIBF1 protein, human; D011257:Pregnancy Proteins; D034741:RNA, Small Interfering; D054411:Receptors, Leptin; D013491:Suppressor Factors, Immunologic; D011374:Progesterone