title: Developmental patterns of PPAR and RXR gene expression during spermatogenesis.
abstract: Members of the family of nuclear receptors that include peroxisome proliferator-activated receptors (PPARs) and retinoid X receptors (RXRs) are important mediators of selective gene activation during development and cellular differentiation. In this study, developmentally-specific PPAR and RXR patterns of expression that occur in somatic and germ cell populations in the testis were determined using quantative real-time PCR (qRT-PCR) studies on RNAs that were isolated from StaPut purified mouse germ cells and primary rat Sertoli cells. These qRT-PCR studies indicate that transcripts encoding the PPAR-Alpha (α), -Beta (β), and -Gamma (γ) and RXR -Alpha (α), -Beta (β), and -Gamma (γ) are developmentally expressed in both differentiating germ and Sertoli cells. In further experiments aimed at deciphering the physiological role that PPAR-Gamma (γ) plays in Sertoli cells, 15-day primary rat Sertoli cells were infected with recombinant adenoviral vectors containing PPAR-Gamma (γ) cDNA and PPAR-Gamma (γ) RNAi constructs. Affymetrix microarray analysis and qRT-PCR validation studies using total RNA isolated from these transfected cells indicated that PPAR-Gamma regulates the pattern of expression of key lipid metabolic genes in Sertoli cells.
authors.affiliation: Department of Anatomy and Neurobiology, Morehouse School of Medicine, Atlanta, GA 30310-1495, USA. kethomas@msm.edu
authors.forename: Kelwyn
authors.initials: K
authors.lastname: Thomas
authors.forename: Dae-Yong
authors.initials: DY
authors.lastname: Sung
authors.forename: Xing
authors.initials: X
authors.lastname: Chen
authors.forename: Winston
authors.initials: W
authors.lastname: Thompson
authors.forename: Y Eugene
authors.initials: YE
authors.lastname: Chen
authors.forename: John
authors.initials: J
authors.lastname: McCarrey
authors.forename: William
authors.initials: W
authors.lastname: Walker
authors.forename: Michael
authors.initials: M
authors.lastname: Griswold
mesh_terms: D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D017931:DNA Primers; D018507:Gene Expression Regulation, Developmental; D008297:Male; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D047492:Peroxisome Proliferator-Activated Receptors / Q000235:genetics*; D016133:Polymerase Chain Reaction; D051381:Rats; D017207:Rats, Sprague-Dawley; D047488:Retinoid X Receptors / Q000235:genetics*; D013091:Spermatogenesis / Q000235:genetics*
chemical_list: D017931:DNA Primers; D047492:Peroxisome Proliferator-Activated Receptors; D047488:Retinoid X Receptors