title: Distal end of carboxyl terminus is not essential for the assembly of rat Eag1 potassium channels.
abstract: The assembly of four pore-forming α-subunits into tetramers is a prerequisite for the formation of functional K(+) channels. A short carboxyl assembly domain (CAD) in the distal end of the cytoplasmic carboxyl terminus has been implicated in the assembly of Eag α-subunits, a subfamily of the ether-à-go-go K(+) channel family. The precise role of CAD in the formation of Eag tetrameric channels, however, remains unclear. Moreover, it has not been determined whether other protein regions also contribute to the assembly of Eag subunits. We addressed these questions by studying the biophysical properties of a series of different rat Eag1 (rEag1) truncation mutants. Two truncation mutants without CAD (K848X and W823X) yielded functional phenotypes similar to those for wild-type (WT) rEag1 channels. Furthermore, nonfunctional rEag1 truncation mutants lacking the distal region of the carboxyl terminus displayed substantial dominant-negative effects on the functional expression of WT as well as K848X and W823X channels. Our co-immunoprecipitation studies further revealed that truncation mutants containing no CAD indeed displayed significant association with rEag1-WT subunits. Finally, surface biotinylation and protein glycosylation analyses demonstrated that progressive truncations of the carboxyl terminus resulted in aggravating disruptions of membrane trafficking and glycosylation of rEag1 proteins. Overall, our data suggest that the distal carboxyl terminus, including CAD, is dispensable for the assembly of rEag1 K(+) channels but may instead be essential for ensuring proper protein biosynthesis. We propose that the S6 segment and the proximal carboxyl terminus may constitute the principal subunit recognition site for the assembly of rEag1 channels.
authors.affiliation: Institute of Anatomy and Cell Biology, School of Medicine, National Yang-Ming University, Taipei 12212, Taiwan.
authors.forename: I-Hsiu
authors.initials: IH
authors.lastname: Chen
authors.forename: Jui-Hsiang
authors.initials: JH
authors.lastname: Hu
authors.forename: Guey-Mei
authors.initials: GM
authors.lastname: Jow
authors.forename: Chao-Chin
authors.initials: CC
authors.lastname: Chuang
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Lee
authors.forename: Dai-Chi
authors.initials: DC
authors.lastname: Liu
authors.forename: Chung-Jiuan
authors.initials: CJ
authors.lastname: Jeng
mesh_terms: D000818:Animals; D002460:Cell Line; D018076:DNA, Complementary; D051638:Ether-A-Go-Go Potassium Channels / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D005455:Fluorescent Antibody Technique; D006031:Glycosylation; D006801:Humans; D009154:Mutation; D018408:Patch-Clamp Techniques; D051381:Rats; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D051638:Ether-A-Go-Go Potassium Channels; C496886:Kcnh1 protein, rat; D011994:Recombinant Proteins