title: [Role of glucocorticoid-induced tumor necrosis factor-related protein ligand (GITRL) on lipopolysaccharide indu-ced Kupffer cells apoptosis].
abstract: OBJECTIVE
  To study the role of glucocorticoid-induced tumor necrosis factor-related protein ligand (GITRL) on apoptosis of mouse Kupffer cells (KCs) induced by lipopolysaccharide (LPS).
  
  
  METHODS
  The KCs were isolated from BALB/c mice and transfected with Control siRNA or GITRL siRNA for 24 h. The KCs were randomly divided into four groups including control group: cultured in media alone, dexamethasone (Dex) group: media with Dex 10 μmol/L, LPS group: media with LPS 1 mg/L, and LPS+Dex group: media with LPS 1 mg/L and Dex 10 μmol/L. At 24 h after treatment, the expression of GITRL was detected by immunocytochemistry. The apoptosis of KCs was measured by Annexin V-FITC/PI double staining and FCM.
  
  
  RESULTS
  The GITRL expression of KCs was increased by LPS challenge (P < 0.05), whereas Dex treatment attenuated the increase. LPS challenge induced KCs apoptosis, but the LPS induced apoptosis was inhibited by GITRL siRNA transfection or Dex treatment (P < 0.05, respectively).
  
  
  CONCLUSIONS
  LPS could induce mouse KCs apoptosis, which may be depend on GITRL signal transduction.
authors.affiliation: Chongqing Key Laboratory of Hepatobiliary Surgery and Department of Hepatobiliary Surgery, Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, China. weisidongyishi@126.com
authors.forename: Si-dong
authors.initials: SD
authors.lastname: Wei
authors.forename: Jin-zheng
authors.initials: JZ
authors.lastname: Li
authors.forename: Jian-ping
authors.initials: JP
authors.lastname: Gong
authors.forename: Zuo-jin
authors.initials: ZJ
authors.lastname: Liu
authors.forename: Hai-bo
authors.initials: HB
authors.lastname: You
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
authors.forename: Chuan-xin
authors.initials: CX
authors.lastname: Wu
mesh_terms: D000818:Animals; D017209:Apoptosis; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology*; D053450:Glucocorticoid-Induced TNFR-Related Protein / Q000378:metabolism*; D007728:Kupffer Cells / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D034741:RNA, Small Interfering; D018124:Receptors, Tumor Necrosis Factor / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D053450:Glucocorticoid-Induced TNFR-Related Protein; D008070:Lipopolysaccharides; D034741:RNA, Small Interfering; D018124:Receptors, Tumor Necrosis Factor; C106544:Tnfrsf18 protein, mouse; D003907:Dexamethasone