title: What's in a drop? Correlating observations and outcomes to guide macromolecular crystallization experiments.
abstract: Observations of crystallization experiments are classified as specific outcomes and integrated through a phase diagram to visualize solubility and thereby direct subsequent experiments. Specific examples are taken from our high-throughput crystallization laboratory which provided a broad scope of data from 20 million crystallization experiments on 12,500 different biological macromolecules. The methods and rationale are broadly and generally applicable in any crystallization laboratory. Through a combination of incomplete factorial sampling of crystallization cocktails, standard outcome classifications, visualization of outcomes as they relate chemically and application of a simple phase diagram approach we demonstrate how to logically design subsequent crystallization experiments.
authors.affiliation: Hauptman-Woodward Medical Research Institute, 700 Ellicott St., Buffalo, NY 14203, USA.
authors.forename: Joseph R
authors.initials: JR
authors.lastname: Luft
authors.forename: Jennifer R
authors.initials: JR
authors.lastname: Wolfley
authors.forename: Edward H
authors.initials: EH
authors.lastname: Snell