title: Girdin is an intrinsic regulator of neuroblast chain migration in the rostral migratory stream of the postnatal brain.
abstract: In postnatally developing and adult brains, interneurons of the olfactory bulb (OB) are continuously generated at the subventricular zone of the forebrain. The newborn neuroblasts migrate tangentially to the OB through a well defined pathway, the rostral migratory stream (RMS), where the neuroblasts undergo collective migration termed "chain migration." The cell-intrinsic regulatory mechanism of neuroblast chain migration, however, has not been uncovered. Here we show that mice lacking the actin-binding Akt substrate Girdin (a protein that interacts with Disrupted-In-Schizophrenia 1 to regulate neurogenesis in the dentate gyrus) have profound defects in neuroblast chain migration along the RMS. Analysis of two gene knock-in mice harboring Girdin mutants identified unique amino acid residues in Girdin's C-terminal domain that are responsible for the regulation of neuroblast chain migration but revealed no apparent requirement of Girdin phosphorylation by Akt. Electron microscopic analyses demonstrated the involvement of Girdin in neuroblast cell-cell interactions. These findings suggest that Girdin is an important intrinsic factor that specifically governs neuroblast chain migration along the RMS.
authors.affiliation: Department of Pathology and Cell Pharmacology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan.
authors.forename: Yun
authors.initials: Y
authors.lastname: Wang
authors.forename: Naoko
authors.initials: N
authors.lastname: Kaneko
authors.forename: Naoya
authors.initials: N
authors.lastname: Asai
authors.forename: Atsushi
authors.initials: A
authors.lastname: Enomoto
authors.forename: Mayu
authors.initials: M
authors.lastname: Isotani-Sakakibara
authors.forename: Takuya
authors.initials: T
authors.lastname: Kato
authors.forename: Masato
authors.initials: M
authors.lastname: Asai
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Murakumo
authors.forename: Haruko
authors.initials: H
authors.lastname: Ota
authors.forename: Takao
authors.initials: T
authors.lastname: Hikita
authors.forename: Takashi
authors.initials: T
authors.lastname: Namba
authors.forename: Keisuke
authors.initials: K
authors.lastname: Kuroda
authors.forename: Kozo
authors.initials: K
authors.lastname: Kaibuchi
authors.forename: Guo-li
authors.initials: GL
authors.lastname: Ming
authors.forename: Hongjun
authors.initials: H
authors.lastname: Song
authors.forename: Kazunobu
authors.initials: K
authors.lastname: Sawamoto
authors.forename: Masahide
authors.initials: M
authors.lastname: Takahashi
mesh_terms: D000818:Animals; D001921:Brain / Q000033:anatomy & histology / Q000254:growth & development / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D002465:Cell Movement / Q000235:genetics / Q000502:physiology*; D002478:Cells, Cultured; D055879:Gene Knock-In Techniques / Q000379:methods; D007365:Intercellular Junctions / Q000235:genetics / Q000648:ultrastructure; D007395:Interneurons / Q000378:metabolism / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D009154:Mutation; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000502:physiology; D058953:Neural Stem Cells / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D009830:Olfactory Bulb / Q000033:anatomy & histology / Q000254:growth & development / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D033921:Vesicular Transport Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
chemical_list: D008840:Microfilament Proteins; D009419:Nerve Tissue Proteins; D033921:Vesicular Transport Proteins; C498794:Disc1 protein, mouse; C527550:Ccdc88a protein, mouse