title: Label-free electrochemical aptasensor for thrombin detection based on the nafion@graphene as platform.
abstract: A sensitive label-free electrochemical aptasensor was successfully fabricated for thrombin detection with nafion@graphene as platform. With electrostatic interaction between nafion and methylene blue (MB), positive charged MB was successfully assembled on nafion@graphene modified electrode surface, which provided amounts of redox probes for electrochemical aptasensor. In the presence of thrombin, the thrombin aptamer (TBA) on the electrode surface would catch the target on the electrode interface, which made a barrier for electrons and inhibits the electro-transfer, resulting in the decreased differential pulse voltammetry signals of MB. As a result, the proposed approach showed a high sensitivity and a wider linearity to thrombin in the range 0.01-50 nM with a detection limit of 6 pM.
authors.affiliation: College of Chemistry and Chemical Engineering, Yibin University, Yibin, 644000, Sichuan, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Sun
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.forename: Xianxue
authors.initials: X
authors.lastname: Gan
mesh_terms: D015374:Biosensing Techniques; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D004867:Equipment Design; D005465:Fluorocarbon Polymers / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry; D008751:Methylene Blue / Q000032:analysis / Q000737:chemistry; D010084:Oxidation-Reduction; D012680:Sensitivity and Specificity; D055672:Static Electricity; D013917:Thrombin / Q000032:analysis* / Q000737:chemistry; D013997:Time Factors
chemical_list: D005465:Fluorocarbon Polymers; D007074:Immunoglobulin G; C040402:perfluorosulfonic acid; D006046:Gold; D006108:Graphite; D013917:Thrombin; D008751:Methylene Blue