title: Lipoxygenase in Caragana jubata responds to low temperature, abscisic acid, methyl jasmonate and salicylic acid.
abstract: Lipoxygenase (LOX) catalyses oxygenation of free polyunsaturated fatty acids into oxylipins, and is a critical enzyme of the jasmonate signaling pathway. LOX has been shown to be associated with biotic and abiotic stress responses in diverse plant species, though limited data is available with respect to low temperature and the associated cues. Using rapid amplification of cDNA ends, a full-length cDNA (CjLOX) encoding lipoxygenase was cloned from apical buds of Caragana jubata, a temperate plant species that grows under extreme cold. The cDNA obtained was 2952bp long consisting of an open reading frame of 2610bp encoding 869 amino acids protein. Multiple alignment of the deduced amino acid sequence with those of other plants demonstrated putative LH2/ PLAT domain, lipoxygenase iron binding catalytic domain and lipoxygenase_2 signature sequences. CjLOX exhibited up- and down-regulation of gene expression pattern in response to low temperature (LT), abscisic acid (ABA), methyl jasmonate (MJ) and salicylic acid (SA). Among all the treatments, a strong up-regulation was observed in response to MJ. Data suggests an important role of jasmonate signaling pathway in response to LT in C. jubata.
authors.affiliation: Biotechnology Division, Institute of Himalayan Bioresource Technology, Council of Scientific and Industrial Research, Himachal Pradesh, India.
authors.forename: Pardeep Kumar
authors.initials: PK
authors.lastname: Bhardwaj
authors.forename: Jagdeep
authors.initials: J
authors.lastname: Kaur
authors.forename: Ranbir Chander
authors.initials: RC
authors.lastname: Sobti
authors.forename: Paramvir Singh
authors.initials: PS
authors.lastname: Ahuja
authors.forename: Sanjay
authors.initials: S
authors.lastname: Kumar
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology*; D000085:Acetates / Q000494:pharmacology*; D000595:Amino Acid Sequence; D032602:Caragana / Q000235:genetics*; D003080:Cold Temperature; D003517:Cyclopentanes / Q000378:metabolism* / Q000494:pharmacology*; D017343:Genes, Plant; D008084:Lipoxygenase / Q000378:metabolism*; D008969:Molecular Sequence Data; D054883:Oxylipins / Q000378:metabolism* / Q000494:pharmacology*; D010802:Phylogeny; D020156:Salicylic Acid / Q000494:pharmacology*; D016415:Sequence Alignment; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D054883:Oxylipins; C011006:jasmonic acid; D000040:Abscisic Acid; C072239:methyl jasmonate; D008084:Lipoxygenase; D020156:Salicylic Acid