title: Detection of methylated tissue factor pathway inhibitor 2 and human long DNA in fecal samples of patients with colorectal cancer in China.
abstract: BACKGROUND
  To investigate the feasibility of detecting methylated tissue factor pathway inhibitor (TFPI2) and quantifying human long DNA with fluorescent quantitative Alu PCR in fecal DNA as a non-invasive screening tool for colorectal cancer (CRC).
  
  
  METHODS
  Methylation-specific PCR (MSP) was performed to analyze TFPI2 gene promoter methylation status in a blinded fashion in stool samples taken from 30 endoscopically diagnosed healthy controls, 20 patients with adenomas, and 60 patients with colorectal cancer. Real-time Alu PCR was used to quantify human long DNA.
  
  
  RESULTS
  The specificity of fecal TFPI2 MSP assay and long DNA assay was 100% and 83.3%, respectively. The sensitivity of fecal TFPI2 MSP assay and long DNA assay was 68.3% and 53.3%, respectively. The sensitivity of fecal DNA assay (either marker being positive) was 86.7%, which was high for CRC.
  
  
  CONCLUSIONS
  Our results have demonstrated the feasibility of using TFPI2 methylation and quantify human long DNA with fluorescent quantitative Alu PCR in fecal samples as a new noninvasive test for CRC.
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
authors.forename: JinPing
authors.initials: J
authors.lastname: Zhang
authors.forename: ShaoBin
authors.initials: S
authors.lastname: Yang
authors.forename: YuanYuan
authors.initials: Y
authors.lastname: Xie
authors.forename: XiangYu
authors.initials: X
authors.lastname: Chen
authors.forename: Ye
authors.initials: Y
authors.lastname: Zhao
authors.forename: DeZhi
authors.initials: D
authors.lastname: He
authors.forename: JianSheng
authors.initials: J
authors.lastname: Li
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D002681:China; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000378:metabolism; D019175:DNA Methylation; D004273:DNA, Neoplasm / Q000032:analysis* / Q000235:genetics; D055088:Early Detection of Cancer / Q000379:methods; D005243:Feces / Q000737:chemistry; D005260:Female; D006023:Glycoproteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D009780:Occult Blood; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm; D006023:Glycoproteins; C021240:tissue-factor-pathway inhibitor 2