title: Deep sequencing reveals novel microRNAs and regulation of microRNA expression during cell senescence.
abstract: In cell senescence, cultured cells cease proliferating and acquire aberrant gene expression patterns. MicroRNAs (miRNAs) modulate gene expression through translational repression or mRNA degradation and have been implicated in senescence. We used deep sequencing to carry out a comprehensive survey of miRNA expression and involvement in cell senescence. Informatic analysis of small RNA sequence datasets from young and senescent IMR90 human fibroblasts identifies many miRNAs that are regulated (either up or down) with cell senescence. Comparison with mRNA expression profiles reveals potential mRNA targets of these senescence-regulated miRNAs. The target mRNAs are enriched for genes involved in biological processes associated with cell senescence. This result greatly extends existing information on the role of miRNAs in cell senescence and is consistent with miRNAs having a causal role in the process.
authors.affiliation: Center for Genetics, Children's Hospital Oakland Research Institute, Oakland, California, United States of America. jdhahbi@chori.org
authors.forename: Joseph M
authors.initials: JM
authors.lastname: Dhahbi
authors.forename: Hani
authors.initials: H
authors.lastname: Atamna
authors.forename: Dario
authors.initials: D
authors.lastname: Boffelli
authors.forename: Wendy
authors.initials: W
authors.lastname: Magis
authors.forename: Stephen R
authors.initials: SR
authors.lastname: Spindler
authors.forename: David I K
authors.initials: DI
authors.lastname: Martin
mesh_terms: D001686:Biological Phenomena / Q000235:genetics; D016922:Cellular Senescence / Q000235:genetics*; D016000:Cluster Analysis; D030541:Databases, Genetic; D015536:Down-Regulation / Q000235:genetics; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D058977:Molecular Sequence Annotation; D015854:Up-Regulation / Q000235:genetics
chemical_list: D035683:MicroRNAs