title: Immobilization of Ag nanoparticles/FGF-2 on a modified titanium implant surface and improved human gingival fibroblasts behavior.
abstract: The objective of this study was to form a rapid and firm soft tissue sealing around dental implants that resists bacterial invasion. We present a novel approach to modify Ti surface by immobilizing Ag nanoparticles/FGF-2 compound bioactive factors onto a titania nanotubular surface. The titanium samples were anodized to form vertically organized TiO(2) nanotube arrays and Ag nanoparticles were electrodeposited onto the nanotubular surface, on which FGF-2 was immobilized with repeated lyophilization. A uniform distribution of Ag nanoparticles/FGF-2 was observed on the TiO(2) nanotubular surface. The L929 cell line was used for cytotoxicity assessment. Human gingival fibroblasts (HGFs) were cultured on the modified surface for cytocompatibility determination. The Ag/FGF-2 immobilized samples displayed excellent cytocompatibility, negligible cytotoxicity, and enhanced HGF functions such as cell attachment, proliferation, and ECM-related gene expression. The Ag nanoparticles also exhibit some bioactivity. In conclusion, this modified TiO(2) nanotubular surface has a large potential for use in dental implant abutment.
authors.affiliation: School of Stomatology, The Fourth Military Medical University, 17 West Chang Le Road, Xi'an 710032, China.
authors.forename: Qianli
authors.initials: Q
authors.lastname: Ma
authors.forename: Shenglin
authors.initials: S
authors.lastname: Mei
authors.forename: Kun
authors.initials: K
authors.lastname: Ji
authors.forename: Yumei
authors.initials: Y
authors.lastname: Zhang
authors.forename: Paul K
authors.initials: PK
authors.lastname: Chu
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D015921:Dental Implants; D005109:Extracellular Matrix / Q000187:drug effects / Q000235:genetics; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects*; D005786:Gene Expression Regulation / Q000187:drug effects; D005881:Gingiva / Q000166:cytology; D006801:Humans; D055767:Immobilized Proteins / Q000494:pharmacology; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D051379:Mice; D056951:Photoelectron Spectroscopy; D012834:Silver / Q000494:pharmacology*; D013499:Surface Properties / Q000187:drug effects; D013816:Thermodynamics; D014025:Titanium / Q000494:pharmacology*; D055815:Young Adult
chemical_list: D015921:Dental Implants; D055767:Immobilized Proteins; D016222:Fibroblast Growth Factor 2; D012834:Silver; D014025:Titanium