title: Single dose adenovirus vectored vaccine induces a potent and long-lasting immune response against rabbit hemorrhagic disease virus after parenteral or mucosal administration.
abstract: Rabbit hemorrhagic disease virus (RHDV) is the etiological agent of a lethal and contagious disease of rabbits that remains as a serious problem worldwide. As this virus does not replicate in cell culture systems, the capsid protein gene has been expressed in heterologous hosts or inserted in replication-competent viruses in order to obtain non-conventional RHDV vaccines. However, due to technological or safety issues, current RHDV vaccines are still prepared from organs of infected rabbits. In this work, two human type 5 derived replication-defective adenoviruses encoding the rabbit hemorrhagic disease virus VP60 capsid protein were constructed. The recombinant protein was expressed as a multimer in mouse and rabbit cell lines at levels that ranged from approximately 120 to 160 mg/L of culture. Mice intravenously or subcutaneously inoculated with a single 10(8) gene transfer units (GTU) dose of the AdVP60 vector (designed for VP60 intracellular expression) seroconverted at days 7 and 14 post-immunization, respectively. This vector generated a stronger response than that obtained with a second vector (AdVP60sec) designed for VP60 secretion. Rabbits were then immunized by parenteral or mucosal routes with a single 10(9)GTU dose of the AdVP60 and the antibody response was evaluated using a competition ELISA specific for RHDV or RHDVa. Protective hemagglutination inhibition (HI) titers were also promptly detected and IgG antibodies corresponding with inhibition percentages over 85% persisted up to one year in all rabbits, independently of the immunization route employed. These levels were similar to those elicited with inactivated RHDV or with VP60 obtained from yeast or insect cells. IgA specific antibodies were only found in saliva of rabbits immunized by intranasal instillation. The feasibility of VP60 production and vaccination of rabbits with replication-defective adenoviral vectors was demonstrated.
authors.affiliation: Animal Biotechnology Division, Center for Genetic Engineering and Biotechnology, PO Box 6162, CP 10600, Havana, Cuba.
authors.forename: Erlinda
authors.initials: E
authors.lastname: Fernández
authors.forename: Jorge R
authors.initials: JR
authors.lastname: Toledo
authors.forename: Maylin
authors.initials: M
authors.lastname: Chiong
authors.forename: Francisco
authors.initials: F
authors.lastname: Parra
authors.forename: Elsa
authors.initials: E
authors.lastname: Rodríguez
authors.forename: Carlos
authors.initials: C
authors.lastname: Montero
authors.forename: Lídice
authors.initials: L
authors.lastname: Méndez
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Capucci
authors.forename: Omar
authors.initials: O
authors.lastname: Farnós
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D058956:Administration, Mucosal; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D017250:Caliciviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary* / Q000821:virology; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D005822:Genetic Vectors / Q000235:genetics / Q000276:immunology*; D006385:Hemagglutination Inhibition Tests / Q000662:veterinary; D017929:Hemorrhagic Disease Virus, Rabbit / Q000235:genetics / Q000276:immunology*; D007114:Immunization / Q000379:methods / Q000662:veterinary; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits / Q000276:immunology* / Q000821:virology; D011897:Random Allocation; D018709:Statistics, Nonparametric; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D015678:Viral Structural Proteins / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D014614:Vaccines, Synthetic; D015678:Viral Structural Proteins; D014765:Viral Vaccines; C081149:viral protein 60, rabbit hemorrhagic disease virus