title: Goose haemorrhagic hepatitis caused by a new subtype duck hepatitis type 1 virus.
abstract: Duck hepatitis type 1 virus (DHV-1) causes a fatal disease in ducklings but there is no report of DHV-1 isolation from goose. Recently, cases of a new disease in overfeeding geese were reported from China. The cases were characterized by haemorrhagic hepatitis lesions on liver after post mortem examinations. The flocks showed about 20-40% morbidity and less than 5% mortality. The histopathological lesions showed destroyed structure of hepatocytic tissue, severe vacuolation and necrosis of hepatocytes. Viral antigen could be detected by monoclonal antibody against duck hepatitis type 1 virus (DHV-1) in the cytoplasm of positive hepatocytes. PCR amplified viral sequences with primers specific for recent Korean-like duck hepatitis type 1 virus (DHV-1C). Alignment of the complete sequence demonstrated that the isolated JT strain from goose exhibiting 95.9% identity to DHV-1C AP-03337 strain, and only 75.3% to classical DHV-1 virus. 80% goslings developed haemorrhagic hepatitis after infection with JT strain. This is the first report on the involvement of a DHV-1 virus in goose.
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, PR China.
authors.forename: Ming
authors.initials: M
authors.lastname: Liu
authors.lastname: Fanyi-Meng
authors.forename: Xiao-jun
authors.initials: XJ
authors.lastname: Li
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Zhang
authors.forename: Siguo
authors.initials: S
authors.lastname: Liu
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002681:China; D005777:Geese; D015611:Hepatitis B Virus, Duck / Q000502:physiology*; D004371:Hepatitis Virus, Duck / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D006520:Hepatitis, Animal / Q000821:virology*; D016133:Polymerase Chain Reaction; D011201:Poultry Diseases / Q000473:pathology / Q000821:virology*