title: Integration of carboxyl modified magnetic particles and aqueous two-phase extraction for selective separation of proteins.
abstract: Both of the magnetic particle adsorption and aqueous two-phase extraction (ATPE) were simple, fast and low-cost method for protein separation. Selective proteins adsorption by carboxyl modified magnetic particles was investigated according to protein isoelectric point, solution pH and ionic strength. Aqueous two-phase system of PEG/sulphate exhibited selective separation and extraction for proteins before and after magnetic adsorption. The two combination ways, magnetic adsorption followed by ATPE and ATPE followed by magnetic adsorption, for the separation of proteins mixture of lysozyme, bovine serum albumin, trypsin, cytochrome C and myloglobin were discussed and compared. The way of magnetic adsorption followed by ATPE was also applied to human serum separation.
authors.affiliation: School of Life Science, Beijing Institute of Technology, Beijing 100081, PR China.
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Gai
authors.forename: Feng
authors.initials: F
authors.lastname: Qu
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.forename: Yukui
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000327:Adsorption; D000818:Animals; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D008280:Magnetics / Q000379:methods*; D008722:Methods; D009994:Osmolar Concentration; D011506:Proteins / Q000302:isolation & purification*; D012996:Solutions / Q000737:chemistry*
chemical_list: D011506:Proteins; D012996:Solutions