title: Bottom-up shotgun lipidomics by higher energy collisional dissociation on LTQ Orbitrap mass spectrometers.
abstract: Higher energy collision dissociation (HCD) is a complementary fragmentation tool that has recently become available on mass spectrometers of the LTQ Orbitrap family. We report on a shotgun bottom-up lipidomics approach that relies on HCD of the isolated lipid precursors. HCD, together with the high mass resolution and mass accuracy of the Orbitrap analyzer, improved the confidence of molecular species assignment and accuracy of their quantification in total lipid extracts. These capabilities were particularly important for accounting for biologically interesting lipid species comprising polyunsaturated and odd numbered fatty acid moieties. We argue that now both bottom-up and top-down shotgun lipidomics could be performed on the same instrumentation platform.
authors.affiliation: Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
authors.forename: Kai
authors.initials: K
authors.lastname: Schuhmann
authors.forename: Ronny
authors.initials: R
authors.lastname: Herzog
authors.forename: Dominik
authors.initials: D
authors.lastname: Schwudke
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Metelmann-Strupat
authors.forename: Stefan R
authors.initials: SR
authors.lastname: Bornstein
authors.forename: Andrej
authors.initials: A
authors.lastname: Shevchenko
mesh_terms: D000818:Animals; D019295:Computational Biology / Q000379:methods; D020404:Glycerophospholipids / Q000032:analysis / Q000737:chemistry; D008055:Lipids / Q000032:analysis* / Q000737:chemistry; D051381:Rats; D012160:Retina / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D020404:Glycerophospholipids; D008055:Lipids