title: Akt phosphorylates and regulates the function of Dlx5.
abstract: Akt, a phosphoinositide-dependent serine/threonine protein kinase, acts as a key regulator in bone formation. Akt can be activated by several osteogenic signaling molecules, but its precise function and downstream targets in bone development are unknown. Dlx5 transcription factor plays important roles during bone development and osteoblast differentiation. Its expression is regulated by several osteogenic signals. In addition, Dlx5 function is also regulated through post-translational modification by several kinases. In this report, we have investigated a potential regulation of Dlx5 function by Akt. Our results indicate that Akt interacts with and phosphorylates Dlx5. In addition, we provide evidences that Akt kinase activity is important for Akt to enhance the protein stability and transcriptional activity of Dlx5. These results suggest that Dlx5 is a novel target of Akt and that the activity of Dlx5 could be modulated by a novel mechanism involving Akt during osteoblast differentiation.
authors.affiliation: College of Pharmacy and Research Institute of Drug Development, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Hyung Min
authors.initials: HM
authors.lastname: Jeong
authors.forename: Yun-Hye
authors.initials: YH
authors.lastname: Jin
authors.forename: Yeon-Jin
authors.initials: YJ
authors.lastname: Kim
authors.forename: Jinah
authors.initials: J
authors.lastname: Yum
authors.forename: You Hee
authors.initials: YH
authors.lastname: Choi
authors.forename: Chang-Yeol
authors.initials: CY
authors.lastname: Yeo
authors.forename: Kwang-Youl
authors.initials: KY
authors.lastname: Lee
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D002460:Cell Line; D018398:Homeodomain Proteins / Q000378:metabolism*; D006801:Humans; D051379:Mice; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D010012:Osteogenesis / Q000235:genetics; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D055550:Protein Stability; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: C501120:Dlx5 protein, mouse; D018398:Homeodomain Proteins; D051057:Proto-Oncogene Proteins c-akt