title: Comparative proteome analyses of host protein expression in response to Enterovirus 71 and Coxsackievirus A16 infections.
abstract: Enterovirus 71 (EV71) and Coxsackievirus A16 (CA16) are the main etiological agents of Hand, Foot and Mouth Disease (HFMD), a common disease among children and had caused several outbreaks in the Asia-Pacific region. Although being genetically close to each other, EV71 infection can cause serious and fatal neurological complications like encephalitis, myocarditis, acute flaccid paralysis (AFP) and aseptic meningitis, but not in CA16 infections. In this study, the cellular response of host cells infected with EV71 and CA16 was characterized and compared by 2-dimensional proteome analyses. A total of 16 proteins were identified to be differentially expressed in EV71 and CA16-infected host cells. Desmin and HSP27, both indirectly regulate the contraction of muscle cells, were significantly downregulated as a result of EV71 infection, suggesting a link to acute flaccid paralysis. The ability of EV71 to evade host immune system may be due to the downregulation of MHC-I synthesis proteins like protein disulfide isomerase A3 and calreticulin. Proteins such as nucleophosmin, nuclear ribonucleoprotein C, and eukaryotic translation initiation factor 2 were all downregulated significantly, suggesting the rapid shutting down of host translation machinery by EV71. These findings provide insight into the nature of high virulent EV71 infection as compared to CA16.
authors.affiliation: Department of Paediatrics, University Children's Medical Institute, National University Hospital, Singapore, Singapore.
authors.forename: Jia Jun
authors.initials: JJ
authors.lastname: Lee
authors.forename: Joey Bing Kai
authors.initials: JB
authors.lastname: Seah
authors.forename: Vincent Tak Kwong
authors.initials: VT
authors.lastname: Chow
authors.forename: Chit Laa
authors.initials: CL
authors.lastname: Poh
authors.forename: Eng Lee
authors.initials: EL
authors.lastname: Tan
mesh_terms: D002648:Child; D002675:Child, Preschool; D003384:Coxsackievirus Infections / Q000235:genetics* / Q000378:metabolism; D003893:Desmin / Q000096:biosynthesis* / Q000235:genetics; D015536:Down-Regulation; D029821:Enterovirus A, Human / Q000235:genetics*; D020869:Gene Expression Profiling; D055551:HSP27 Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D006232:Hand, Foot and Mouth Disease / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D006360:Heat-Shock Proteins; D006801:Humans; D018832:Molecular Chaperones; D020543:Proteome / Q000235:genetics; D012208:Rhabdomyosarcoma / Q000378:metabolism / Q000821:virology; D014407:Tumor Cells, Cultured
chemical_list: D003893:Desmin; D055551:HSP27 Heat-Shock Proteins; C057725:HSPB1 protein, human; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D020543:Proteome