title: Analysis of a viral metagenomic library from 200 m depth in Monterey Bay, California constructed by direct shotgun cloning.
abstract: BACKGROUND
  Viruses have a profound influence on both the ecology and evolution of marine plankton, but the genetic diversity of viral assemblages, particularly those in deeper ocean waters, remains poorly described. Here we report on the construction and analysis of a viral metagenome prepared from below the euphotic zone in a temperate, eutrophic bay of coastal California.
  
  
  METHODS
  We purified viruses from approximately one cubic meter of seawater collected from 200 m depth in Monterey Bay, CA. DNA was extracted from the virus fraction, sheared, and cloned with no prior amplification into a plasmid vector and propagated in E. coli to produce the MBv200m library. Random clones were sequenced by the Sanger method. Sequences were assembled then compared to sequences in GenBank and to other viral metagenomic libraries using BLAST analyses.
  
  
  RESULTS
  Only 26% of the 881 sequences remaining after assembly had significant (E≤0.001) BLAST hits to sequences in the GenBank nr database, with most being matches to bacteria (15%) and viruses (8%). When BLAST analysis included environmental sequences, 74% of sequences in the MBv200m library had a significant match. Most of these hits (70%) were to microbial metagenome sequences and only 0.7% were to sequences from viral metagenomes. Of the 121 sequences with a significant hit to a known virus, 94% matched bacteriophages (Families Podo-, Sipho-, and Myoviridae) and 6% matched viruses of eukaryotes in the Family Phycodnaviridae (5 sequences) or the Mimivirus (2 sequences). The largest percentages of hits to viral genes of known function were to those involved in DNA modification (25%) or structural genes (17%). Based on reciprocal BLAST analyses, the MBv200m library appeared to be most similar to viral metagenomes from two other bays and least similar to a viral metagenome from the Arctic Ocean.
  
  
  CONCLUSIONS
  Direct cloning of DNA from diverse marine viruses was feasible and resulted in a distribution of virus types and functional genes at depth that differed in detail, but were broadly similar to those found in surface marine waters. Targeted viral analyses are useful for identifying those components of the greater marine metagenome that circulate in the subcellular size fraction.
authors.affiliation: Department of Oceanography, University of Hawaii at Manoa, 1000 Pope Road, Honolulu, HI 96822, Hawaii. grieg@hawaii.edu
authors.forename: Grieg F
authors.initials: GF
authors.lastname: Steward
authors.forename: Christina M
authors.initials: CM
authors.lastname: Preston
mesh_terms: D044822:Biodiversity; D002140:California; D003001:Cloning, Molecular / Q000379:methods; D016000:Cluster Analysis; D004267:DNA Viruses / Q000145:classification* / Q000235:genetics*; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D054892:Metagenome; D008969:Molecular Sequence Data; D010802:Phylogeny; D012623:Seawater / Q000821:virology*; D017422:Sequence Analysis, DNA
chemical_list: D004279:DNA, Viral