title: Preparation of poly(vinyltetrazole) chain-grafted poly(glycidymethacrylate-co-ethylenedimethacrylate) beads by surface-initiated atom transfer radical polymerization for the use in weak cation exchange and hydrophilic interaction chromatography.
abstract: A novel stationary phase for weak cation exchange (WCX) and hydrophilic interaction chromatography (HILIC) was prepared with surface-initiated atom transfer radical polymerization (SI-ATRP). Vinyltetrazole was grafted onto the surface of the beads in water medium with the polyglycidylmethacrylate beads (P(GMA/EDMA)) previously modified with 2-bromoisobutryl bromide as the macromolecule initiators and CuCl as catalyst. The poly(vinyltetrazole)-grafted beads obtained with different atom transfer radical polymerization (ATRP) formulations were tried as chromatographic packings in ion-exchange chromatography. The results showed that the prepared columns could separate the tested proteins with high efficiency and high capacity, and the retention time of protein had a positive relationship with increasing the chain lengths of the grafted poly(vinyltetrazole) (PVT). The prepared column was also found to be able to separate nucleosides by hydrophilic interaction chromatographic mode.
authors.affiliation: Key Laboratory of Energy & Chemical Engineering, Ningxia University, Yinchuan 750021, PR China.
authors.forename: Junxia
authors.initials: J
authors.lastname: Hao
authors.forename: Fuqiang
authors.initials: F
authors.lastname: Wang
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Dai
authors.forename: Bolin
authors.initials: B
authors.lastname: Gong
authors.forename: Yinmao
authors.initials: Y
authors.lastname: Wei
mesh_terms: D002845:Chromatography / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000379:methods; D057927:Hydrophobic and Hydrophilic Interactions; D008768:Methylmethacrylates; D008863:Microspheres; D058105:Polymerization; D011108:Polymers / Q000737:chemistry
chemical_list: D008768:Methylmethacrylates; D011108:Polymers; C020347:GMA-EDMA