title: The phosphoenolpyruvate-dependent glucose-phosphotransferase system from Escherichia coli K-12 as the center of a network regulating carbohydrate flux in the cell.
abstract: The phosphoenolpyruvate-(PEP)-dependent-carbohydrate:phosphotransferase systems (PTSs) of enteric bacteria constitute a complex transport and sensory system. Such a PTS usually consists of two cytoplasmic energy-coupling proteins, Enzyme I (EI) and HPr, and one of more than 20 different carbohydrate-specific membrane proteins named Enzyme II (EII), which catalyze the uptake and concomitant phosphorylation of numerous carbohydrates. The most prominent representative is the glucose-PTS, which uses a PTS-typical phosphorylation cascade to transport and phosphorylate glucose. All components of the glucose-PTS interact with a large number of non-PTS proteins to regulate the carbohydrate flux in the bacterial cell. Several aspects of the glucose-PTS have been intensively investigated in various research projects of many groups. In this article we will review our recent findings on a Glc-PTS-dependent metalloprotease, on the interaction of EIICB(Glc) with the regulatory peptide SgrT, on the structure of the membrane spanning C-domain of the glucose transporter and on the modeling approaches of ptsG regulation, respectively, and discuss them in context of general PTS research.
authors.affiliation: University of Osnabrück, Faculty of Biology and Chemistry, Department of Genetics, Barbarastrasse 11, 49076 Osnabrück, Germany.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Gabor
authors.forename: Anna-Katharina
authors.initials: AK
authors.lastname: Göhler
authors.forename: Anne
authors.initials: A
authors.lastname: Kosfeld
authors.forename: Ariane
authors.initials: A
authors.lastname: Staab
authors.forename: Andreas
authors.initials: A
authors.lastname: Kremling
authors.forename: Knut
authors.initials: K
authors.lastname: Jahreis
mesh_terms: D050260:Carbohydrate Metabolism; D048168:Escherichia coli K12 / Q000201:enzymology / Q000378:metabolism*; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000378:metabolism*; D010766:Phosphorylation; D015398:Signal Transduction
chemical_list: D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; C031629:phosphoenolpyruvate-glucose phosphotransferase