title: Biochemical analysis and lipid peroxidation in liver ischemic preconditioning.
abstract: BACKGROUND
  Hepatic surgery requires, under diverse circumstances, periods of ischemia and reperfusion (I-R) such as those present in liver resection, hepatic injury, and liver transplantation. The objective of the present work was to conduct an experimental study to evaluate the effect of hepatic preconditioning (HPC) on modulation of the I-R injury.
  
  
  METHODS
  Male Wistar rats were distributed into the following three study groups: group 1, simulated or sham; group 2, submitted to a 30-min period of total warm ischemia and a reperfusion phase, and group 3, in which we carried out 10-min preconditioning of warm ischemia and 10 min of reperfusion prior to the total ischemia period for a total of 60 min and the reperfusion phase. We obtained liver biopsies for thiobarbituric acid reactive substances (TBARS) (MDA, thiobarbituric acid adducts) and for blood sample determinations in serum of liver-cell enzymes such as alanine aminotransferase and aspartate aminotransferase during a 24-h time course.
  
  
  RESULTS
  We observed a decrease in the variables studied in group 3 (HPC) as well as of serum liver enzymes and TBARS levels such as indirect oxidative stress indicators upon comparison with group 2 animals submitted to total hepatic I-R.
  
  
  CONCLUSIONS
  HPC is an efficient surgical strategy for decreasing the elevation of hepatic enzymes and indirect lipoperoxidation indicators in an I-R model. Controlled clinical studies should be performed to determine its functional properties and clinical applicability.
authors.affiliation: Unidad 304, Servicio de Cirugía General, Hospital General de México, Secretaría de Salud, México, D.F., Mexico. montalvoeduardo@hotmail.com
authors.forename: Eduardo Esteban
authors.initials: EE
authors.lastname: Montalvo-Javé
authors.forename: Marco Antonio
authors.initials: MA
authors.lastname: García-Puig
authors.forename: Tomás
authors.initials: T
authors.lastname: Escalante-Tattersfield
authors.forename: Julieta
authors.initials: J
authors.lastname: Peña-Sánchez
authors.forename: Héctor
authors.initials: H
authors.lastname: Vázquez-Meza
authors.forename: José Arturo
authors.initials: JA
authors.lastname: Ortega-Salgado
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D006498:Hepatectomy / Q000009:adverse effects / Q000379:methods*; D019194:Ischemic Preconditioning; D007770:L-Lactate Dehydrogenase / Q000097:blood; D015227:Lipid Peroxidation; D008099:Liver / Q000098:blood supply* / Q000737:chemistry / Q000201:enzymology; D008297:Male; D018384:Oxidative Stress; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000517:prevention & control*; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis*; D052096:Warm Ischemia
chemical_list: D017392:Thiobarbituric Acid Reactive Substances; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase