title: [Effects of garlic oil, age and sex on n-hexane metabolism in rats].
abstract: OBJECTIVE
  To investigate effects of garlic oil (GO), age and sex on n-hexane metabolism in rats.
  
  
  METHODS
  The Wistar rats were used as experimental animals. (1) Intragastric administration: n-hexane group (3000 mg/kg n-hexane), GO treated group (80 mg/kg GO ig. an hour earlier than 3000 mg/kg n-hexane), then blood was taken from tails of rats at 8, 12, 16, 20, 24, 28, 32 h points after n-hexane administration. (2) Intraperitoneal injection: n-hexane group (1000 mg/kg n-hexane), GO treated group (80 mg/kg GO ig. an hour earlier than 1000 mg/kg n-hexane), then took blood was taken from tails of rats at 8, 12, 16, 20, 24, 28 h points after n-hexane injection. (3) 7 rats each group of 6, 8, 10 weeks age were administrated by 3000 mg/kg n-hexane intragastrically, then were taken blood from tails at 16, 20, 24 h points after administration. (4) 7 male and 7 female rats of 8 weeks age were administrated by 3000 mg/kg n-hexane intragastrically, then were taken blood from tails at 16, 20, 24, 28 h points after administration. The gas chromatography was used to determine the metabolite 2, 5-hexanedione concentration of n-hexane in serum and 2, 5-hexanedione concentration was compared between GO and no GO treated rats, different ages and different sexes.
  
  
  RESULTS
  (1) Intragastric administration: 2, 5-hexanedione concentrations in serum of n-hexane group and GO treated group had the peak 19.2 and 12.3 µg/ml at 20h and 24 h points. Compared with n-hexane group, the serum 2, 5-hexanedione concentration of GO treated group was lower at time points prior to peak and 2, 5-hexanedione eliminating process was slower after peak. (2) Intraperitoneal injection: effects of GO on the serum 2, 5-hexanedione concentrations was very similar to intragastric administration, 2, 5-hexanedione concentrations in serum of n-hexane group and GO treated group had the peak 15.0 and 6.7 µg/ml at 12 h and 16 h points. (3) Comparison of the serum 2, 5-hexanedione concentrations of different weeks age rats: The serum 2, 5-hexanedione concentrations of 6, 8, 10 weeks age rats were 25.5, 15.0, 12.8 µg/ml each (8, 10 weeks age significantly lower than 6 weeks age) at 16 h point; at 20 h point, they were 24.7, 18.3, 15.0 µg/ml each (10 weeks age significantly lower than 6 weeks age); at 24 h point, they were 11.0, 14.7, 8.1 µg/ml each (10 weeks age significantly lower than 8 weeks age). (4) Comparisons of the serum 2, 5-hexanedione concentrations of different sex rats: the serum 2, 5-hexanedione concentrations of male and female rats were 22.5, 17.2 µg/ml each at 16 h point (different significantly); at 20, 24, 28 h points, they were 27.6, 22.9 µg/ml, 24.6, 19.1 µg/ml, 19.1, 13.8 µg/ml each (different non-significantly).
  
  
  CONCLUSIONS
  GO reduces production of 2, 5-hexanedione in serum generated by n-hexane in rats; the metabolic capacity of low age rats on n-hexane is stronger than high age ones.
authors.affiliation: Institute of Toxicology, Shandong University, Jinan 250012, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yan
authors.forename: Hong-yin
authors.initials: HY
authors.lastname: Yin
authors.forename: Zhong
authors.initials: Z
authors.lastname: Liu
authors.forename: De-feng
authors.initials: DF
authors.lastname: Chi
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.forename: Qiang-qiang
authors.initials: QQ
authors.lastname: Fu
authors.forename: Ke-qin
authors.initials: KQ
authors.lastname: Xie
mesh_terms: D000367:Age Factors; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D005260:Female; D005737:Garlic; D006586:Hexanes / Q000378:metabolism*; D006588:Hexanones / Q000097:blood; D008297:Male; D010938:Plant Oils / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012737:Sex Factors
chemical_list: D000975:Antioxidants; D006586:Hexanes; D006588:Hexanones; D010938:Plant Oils; C026385:n-hexane; C011269:2,5-hexanedione