title: A novel DTPA cross-linking of hyaluronic acid and metal complexation thereof.
abstract: Macromolecular conjugates of a natural polysaccharide, hyaluronic acid, with diethylenetriaminepentaacetic acid (DTPA)-metal complexes were synthesized and characterized by FTIR, NMR, SEC-MALLS and ICP analysis. Several parameters of the cross-linking reaction as molecular weight of starting HA, temperature, equivalent of DTPA bis-anhydride, concentration of HA, presence of transacylation catalyst DMAP and reaction time were studied. The mechanism for the reaction was suggested and relationship between the molecular weight assigned by SEC-MALLS, reaction parameters and rheological properties of the final cross-linked products were investigated.
authors.affiliation: R&D Department, CPN s.r.o, Dolní Dobrouč 401, Dolní Dobrouč, Czech Republic. buffa@contipro.cz
authors.forename: Radovan
authors.initials: R
authors.lastname: Buffa
authors.forename: Jiří
authors.initials: J
authors.lastname: Běťák
authors.forename: Sofiane
authors.initials: S
authors.lastname: Kettou
authors.forename: Martina
authors.initials: M
authors.lastname: Hermannová
authors.forename: Lucie
authors.initials: L
authors.lastname: Pospíšilová
authors.forename: Vladimír
authors.initials: V
authors.lastname: Velebný
mesh_terms: D006820:Hyaluronic Acid / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D004369:Pentetic Acid / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature
chemical_list: D004369:Pentetic Acid; D006820:Hyaluronic Acid