title: Morphobiometrical and molecular study of two populations of Demodex folliculorum from humans.
abstract: A morphobiometrical and molecular study of two populations of Demodex folliculorum from humans isolated from different habitats, skin and eyelashes follicles, were carried out. Morphological and biometrical studies revealed two closely related populations with any distinctive characteristics. For molecular study, a 436-bp region of the 16S rDNA gene and a 453-bp region of the cytochrome oxidase I (COI) gene from individual mites of each population considered were sequenced. Intraindividual and interindividual sequence variation was studied in both populations. Our data show that 16S rDNA is not a useful marker to discriminate between populations; however, COI gene sequences can help to identify the two populations considered, which are morphologically very close and difficult to separate by classic methods. These results are in agreement with the morphological and biometrical differences detected between D. folliculorum from eyelashes and human skin. This study appeals for the revision of the taxonomic status of the D. folliculorum populations, as well as for the species included within genus Demodex.
authors.affiliation: Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, Profesor García González s/n., 41012, Sevilla, Spain. derojas@us.es
authors.forename: Manuel
authors.initials: M
authors.lastname: de Rojas
authors.forename: Cristina
authors.initials: C
authors.lastname: Riazzo
authors.forename: Rocío
authors.initials: R
authors.lastname: Callejón
authors.forename: Diego
authors.initials: D
authors.lastname: Guevara
authors.forename: Cristina
authors.initials: C
authors.lastname: Cutillas
mesh_terms: D000053:Acari / Q000033:anatomy & histology / Q000145:classification* / Q000235:genetics; D000818:Animals; D001699:Biometry; D016000:Cluster Analysis; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics; D005260:Female; D006801:Humans; D008297:Male; D008924:Mite Infestations / Q000469:parasitology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D003576:Electron Transport Complex IV