title: [High-resolution melting: a analysis for genotyping of MDR1 C3435T in benzene-exposed workers].
abstract: OBJECTIVE
  Using high resolution melting (HRM) to analysis MDR1 C3435T in people exposed to benzene.
  
  
  METHODS
  Restriction fragment length polymorphism (RFLP) was utilized to detect the polymorphism of MDR1 3435 in 121 benzene-exposed workers, and the results were compared with the HRM in 10% samples and were confirmed with direct sequencing for six people in them.
  
  
  RESULTS
  By direct sequencing, consistent results of benzene-exposed workers with RFLP or HRM were got. The new high resolution melting curve analysis is more efficient, more convenient, and cheaper than RFLP.
  
  
  CONCLUSIONS
  High-resolution melting analysis provides a valid approach to efficiently detect DNA genetic diagnosis, which is suitable for detect susceptible genes in occupational surveillance.
authors.affiliation: Department of Occupational Medicine, Fudan University, Shanghai 200540, China.
authors.forename: Jian-shu
authors.initials: JS
authors.lastname: Huang
authors.forename: Xin-ju
authors.initials: XJ
authors.lastname: Zhang
authors.forename: Xiao
authors.initials: X
authors.lastname: Xu
authors.forename: Ming
authors.initials: M
authors.lastname: Guan
authors.forename: Yuan-ling
authors.initials: YL
authors.lastname: Zhou
authors.forename: Ling
authors.initials: L
authors.lastname: Lü
authors.forename: He-jian
authors.initials: HJ
authors.lastname: Zou
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics*; D001554:Benzene; D005838:Genotype; D060005:Genotyping Techniques / Q000379:methods*; D006579:Heterozygote; D006801:Humans; D016273:Occupational Exposure; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide
chemical_list: C513055:ABCB1 protein, human; D018435:ATP Binding Cassette Transporter, Subfamily B; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D001554:Benzene