title: Generation of transgenic dogs that conditionally express green fluorescent protein.
abstract: We report the creation of a transgenic dog that conditionally expresses eGFP (enhanced green fluorescent protein) under the regulation of doxycycline. Briefly, fetal fibroblasts infected with a Tet-on eGFP vector were used for somatic cell nuclear transfer. Subsequently reconstructed oocytes were transferred to recipients. Three clones having transgenes were born and one dog was alive. The dog showed all features of inducible expression of eGFP upon doxycycline administration, and successful breeding resulted in eGFP-positive puppies, confirming stable insertion of the transgene into the genome. This inducible dog model will be useful for a variety of medical research studies.
authors.affiliation: Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, 151-744, Korea.
authors.forename: Min Jung
authors.initials: MJ
authors.lastname: Kim
authors.forename: Hyun Ju
authors.initials: HJ
authors.lastname: Oh
authors.forename: Jung Eun
authors.initials: JE
authors.lastname: Park
authors.forename: Geon A
authors.initials: GA
authors.lastname: Kim
authors.forename: So Gun
authors.initials: SG
authors.lastname: Hong
authors.forename: Goo
authors.initials: G
authors.lastname: Jang
authors.forename: Mo Sun
authors.initials: MS
authors.lastname: Kwon
authors.forename: Bon Chul
authors.initials: BC
authors.lastname: Koo
authors.forename: Teoan
authors.initials: T
authors.lastname: Kim
authors.forename: Sung Keun
authors.initials: SK
authors.lastname: Kang
authors.forename: Jeong Chan
authors.initials: JC
authors.lastname: Ra
authors.forename: Chemyong
authors.initials: C
authors.lastname: Ko
authors.forename: Byeong Chun
authors.initials: BC
authors.lastname: Lee
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics* / Q000378:metabolism; D015139:Blotting, Southern; D015153:Blotting, Western; D017931:DNA Primers / Q000235:genetics; D004285:Dogs; D004318:Doxycycline / Q000008:administration & dosage / Q000494:pharmacology*; D005347:Fibroblasts / Q000378:metabolism; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000187:drug effects*; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D023421:Models, Animal; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D004318:Doxycycline