title: Susceptibility of vertilmicin to modifications by three types of recombinant aminoglycoside-modifying enzymes.
abstract: The susceptibilities of vertilmicin and seven reference aminoglycosides to modifications by six recombinant aminoglycoside-modifying enzymes, AAC(6')-Ie, APH(2'')-Ia, AAC(6')-Ie-APH(2'')-Ia, ANT(2'')-Ia, AAC(6')-Ib, and AAC(6')-Ib-cr, were studied by coupled spectrophotometric assays in microtiter plates. In comparison to other aminoglycosides, the susceptibility of vertilmicin was 45.8- to 250.0-fold lower for AAC(6')-Ie acetylation, 39.2- to 116.7-fold lower for AAC(6')-Ie-APH(2'')-Ia acetylation, and 1.8- to 7.5-fold lower for ANT(2'')-Ia adenylation (except that shown by amikacin) while relatively comparable for AAC(6')-Ib acetylation, AAC(6')-Ib-cr acetylation, APH(2'')-Ia phosphorylation, and AAC(6')-Ie-APH(2'')-Ia phosphorylation.
authors.affiliation: Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
authors.forename: Min
authors.initials: M
authors.lastname: Yuan
authors.forename: Hui
authors.initials: H
authors.lastname: Han
authors.forename: Cong-Ran
authors.initials: CR
authors.lastname: Li
authors.forename: Xin-Yi
authors.initials: XY
authors.lastname: Yang
authors.forename: Guo-Qing
authors.initials: GQ
authors.lastname: Li
authors.forename: Shan
authors.initials: S
authors.lastname: Cen
authors.forename: Xi-Xiong
authors.initials: XX
authors.lastname: Kang
authors.forename: Shu-Yi
authors.initials: SY
authors.lastname: Si
authors.forename: Jian-Dong
authors.initials: JD
authors.lastname: Jiang
authors.forename: Xue-Fu
authors.initials: XF
authors.lastname: You
mesh_terms: D000107:Acetylation; D000123:Acetyltransferases / Q000378:metabolism*; D000617:Aminoglycosides / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D016983:Enterococcus / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D009713:Nucleotidyltransferases / Q000378:metabolism*; D010766:Phosphorylation; D010770:Phosphotransferases / Q000378:metabolism*; D013210:Staphylococcus / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; C487733:vertilmicin; D000123:Acetyltransferases; D010770:Phosphotransferases; D009713:Nucleotidyltransferases