title: Examination of the pharmacokinetics of active ingredients of ginger in humans.
abstract: Ginger extracts have been studied in various clinical trials for different indications. However, the pharmacokinetics of the ginger active constituents in human biological matrices is not well investigated. This study aims to develop a LC-MS/MS method for simultaneous measurement of 6-, 8-, and 10-gingerols and 6-shogaol and study their pharmacokinetics in human plasma and colon tissues. A sensitive LC-MS/MS method was established and validated with a low limit of quantification of 2-5 ng/mL. The intra- and inter-day accuracy ranged from -7.3% to 10.4% and from -9.4% to 9.8%, respectively. The intra- and inter-day precision ranged from 0.9% to 10.9% and from 2.0% to 12.4%, respectively. The glucuronide and sulfate metabolites of 6-, 8-, and 10-gingerols and 6-shogaol in plasma and colon tissues were quantified after hydrolysis with β-glucuronidase and sulfatase. After oral dosing of 2.0 g ginger extracts in human, free 10-gingerol and 6-shogaol were detected in plasma with peak concentrations (9.5 ± 2.2 and 13.6 ± 6.9 ng/mL, respectively) at 1 h after oral administration, but no free 6-gingerol and 8-gingerol were detected in plasma from 0.25 to 24 h. The peak concentrations of glucuronide metabolites of 6-, 8-, and 10-gingerols and 6-shogaol were 0.47 ± 0.31, 0.17 ± 0.14, 0.37 ± 0.19, and 0.73 ± 0.54 μg/mL at 1 h, respectively. The peak concentrations of the sulfate metabolites of 6-, 8-, and 10-gingerols and 6-shogaol were 0.28 ± 0.15, 0.027 ± 0.018, 0.018 ± 0.006, and 0.047 ± 0.035 μg/mL at 1 h, respectively. Very low concentrations (2-3 ng/mL) of 10-gingerol glucuronide and sulfate were found in colon tissues. Pharmacokinetic analysis showed that half-lives of these four analytes and their metabolites were 1-3 h in human plasma. No accumulation was observed for 6-, 8-, and 10-gingerols and 6-shogaol and their metabolites in both plasma and colon tissues after multiple daily dosing.
authors.affiliation: Department of Pharmaceutical Sciences, College of Pharmacy, The University of Michigan, Ann Arbor, 48109-1065, USA.
authors.forename: Yanke
authors.initials: Y
authors.lastname: Yu
authors.forename: Suzanna
authors.initials: S
authors.lastname: Zick
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Li
authors.forename: Peng
authors.initials: P
authors.lastname: Zou
authors.forename: Benjamin
authors.initials: B
authors.lastname: Wright
authors.forename: Duxin
authors.initials: D
authors.lastname: Sun
mesh_terms: D002138:Calibration; D002396:Catechols / Q000097:blood / Q000302:isolation & purification / Q000493:pharmacokinetics*; D002853:Chromatography, Liquid; D003106:Colon / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005233:Fatty Alcohols / Q000097:blood / Q000302:isolation & purification / Q000493:pharmacokinetics*; D020939:Zingiber officinale; D006801:Humans; D008658:Inactivation, Metabolic; D007536:Isomerism; D057230:Limit of Detection; D015394:Molecular Structure; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry; D014018:Tissue Distribution
chemical_list: D002396:Catechols; D005233:Fatty Alcohols; C040115:shogaol; C007845:gingerol