title: Neuroprotective effect of erythropoietin-loaded composite microspheres on retinal ganglion cells in rats.
abstract: This study explored a sustained neuroprotective erythropoietin (EPO) loaded composite microspheres system on injured retinal ganglion cells (RGC). The EPO was first loaded into dextran microparticles to keep its bioactivity using a novel "aqueous-aqueous emulsion" technique. The microspheres were finally formed by encapsulating the microparticles into Poly (DL-lactide-co-glycolide)/Poly (DL-lactide) (PLGA/PLA). A single dose of microspheres was intraperitoneally administrated on the optic nerve crush of rats and compared with multiple doses of EPO solution to investigate the long acting effect of microspheres on RGC. The results demonstrated that the release of microspheres could last for at least 60 days in an in vitro study. The animal experiments showed a similar neuroprotective effect between the single dose microspheres and the multiple doses of EPO solution. So we can draw a conclusion that the EPO-loaded PLGA/PLA microspheres were feasible for neurodegeneration diseases in the retina and central nervous system (CNS).
authors.affiliation: Department of Ophthalmology and Vision Science, Eye and ENT Hospital, Shanghai Medical College, Fudan University, No. 83 Fenyang Road, Shanghai, China.
authors.forename: Xianfang
authors.initials: X
authors.lastname: Rong
authors.forename: Xiaofen
authors.initials: X
authors.lastname: Mo
authors.forename: Tiantian
authors.initials: T
authors.lastname: Ren
authors.forename: Sixing
authors.initials: S
authors.lastname: Yang
authors.forename: Weien
authors.initials: W
authors.lastname: Yuan
authors.forename: Jingyan
authors.initials: J
authors.lastname: Dong
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D003692:Delayed-Action Preparations / Q000008:administration & dosage / Q000494:pharmacology; D003911:Dextrans / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry; D004655:Emulsions / Q000494:pharmacology; D004921:Erythropoietin / Q000008:administration & dosage / Q000494:pharmacology*; D005904:Glial Fibrillary Acidic Protein / Q000235:genetics / Q000378:metabolism; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry; D008297:Male; D008863:Microspheres; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D051381:Rats; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000187:drug effects / Q000378:metabolism; D012165:Retinal Ganglion Cells / Q000187:drug effects* / Q000378:metabolism
chemical_list: D003692:Delayed-Action Preparations; D003911:Dextrans; D004337:Drug Carriers; D004655:Emulsions; D005904:Glial Fibrillary Acidic Protein; D004921:Erythropoietin; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid