title: Novel neutrophil inhibitory factor homologue in the buccal gland secretion of Lampetra japonica.
abstract: Abstract To identify the functional gene fragment, a neutrophil inhibitory factor (NIF) like protein was found in the buccal gland of Lampetra japonica, suggesting that this related lamprey protein represents a novel class of integrin receptor antagonists. The recombinant Lampetra japonica-NIF like (rLj-NIF) was identified by SDS-PAGE and purified by using His·Bind affinity chromatography. Effect of rLj-NIF on neutrophil migration suggested that rLj-NIF can act as a neutrophil inhibitory factor. Besides that, oxidative burst activity of neutriphil was tested by flow cytometry using dihydrorhodamine (DHR123) as a fluorogenic substrate, and the data suggested that the mean fluorescence intensity significantly decreased compared with positive controls (p<0.01). All above results indicated that rLj-NIF could also prevent the binding of β2 integrins to the surface of PMN and its FITC-labeled monoclonal antibodies (p<0.05). These data suggest that Lampetra japonica-NIF like protein is secreted by the stage of the parasite at the site of attachment. rLj-NIF plays an essential role in physiological reaction of neutrophil by a novel class of β2 integrin receptor antagonists. The activity of immunosuppressant of L. japonica-NIF could have potential medicinal value in anti-inflammation and therapy of autoimmune diseases.
authors.affiliation: Institute of Marine Genomics and Proteomics, Liaoning Normal University, Dalian, China. liqw@263.net
authors.forename: Zhuang
authors.initials: Z
authors.lastname: Xue
authors.forename: Jie
authors.initials: J
authors.lastname: Bai
authors.forename: Jing
authors.initials: J
authors.lastname: Sun
authors.forename: Yu
authors.initials: Y
authors.lastname: Wu
authors.forename: Shui Yan
authors.initials: SY
authors.lastname: Yu
authors.forename: Ren Yong
authors.initials: RY
authors.lastname: Guo
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.forename: Qing Wei
authors.initials: QW
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology* / Q000494:pharmacology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D007798:Lampreys / Q000235:genetics* / Q000276:immunology*; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology* / Q000494:pharmacology; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000187:drug effects; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000494:pharmacology; D016897:Respiratory Burst / Q000187:drug effects; D012469:Salivary Glands / Q000276:immunology; D016415:Sequence Alignment
chemical_list: D029941:Fish Proteins; D008565:Membrane Proteins; D011994:Recombinant Proteins