title: Effects of in ovo vaccination and anticoccidials on the distribution of Eimeria spp. in poultry litter and serum antibody titers against coccidia in broiler chickens raised on the used litters.
abstract: The present study reports the effects of various field anticoccidial programs on the distribution of Eimeria spp. in poultry litter and serum antibody titers against coccidia in broiler chickens raised on the used litters. The programs included in ovo vaccination and various medications with either chemicals, ionophores, or both. In general, serum samples from these chickens showed anticoccidial antibody titers when tested at days 7 and 14 post hatch with the peak response at day 43. Serum anticoccidial titers were highest in birds fed a non-medicated diet compared with those vaccinated or fed medicated diets. Total number of Eimeria oocysts and the composition of Eimeria spp. present in the litter samples from different treatment groups varied depending on the type of anticoccidial program. Oocyst counts in general ranged from 3.7×10(3) to 7.0×10(4) per g of litter. Importantly, both morphological and molecular typing studies revealed four major predominant Eimeria spp., E. acervulina, E. maxima, E. praecox, and E. tenella in the litter samples. Collectively, these results indicate that the field anticoccidial programs influenced the type and abundance of Eimeria spp. present in the litter samples and also modulated host immune response to Eimeria.
authors.affiliation: Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, USDA, Beltsville, MD 20705, USA.
authors.forename: Kyung Woo
authors.initials: KW
authors.lastname: Lee
authors.forename: Hyun S
authors.initials: HS
authors.lastname: Lillehoj
authors.forename: Seung I
authors.initials: SI
authors.lastname: Jang
authors.forename: Marc
authors.initials: M
authors.lastname: Pagès
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Bautista
authors.forename: Conrad R
authors.initials: CR
authors.lastname: Pope
authors.forename: G Donald
authors.initials: GD
authors.lastname: Ritter
authors.forename: Erik P
authors.initials: EP
authors.lastname: Lillehoj
authors.forename: Anthony P
authors.initials: AP
authors.lastname: Neumann
authors.forename: Gregory R
authors.initials: GR
authors.lastname: Siragusa
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood* / Q000276:immunology; D002645:Chickens / Q000276:immunology / Q000469:parasitology; D003048:Coccidiosis / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D003049:Coccidiostats / Q000494:pharmacology*; D004539:Eimeria / Q000276:immunology*; D016786:Eimeria tenella / Q000276:immunology; D006799:Housing, Animal; D010063:Ovum / Q000187:drug effects / Q000276:immunology / Q000469:parasitology*; D010270:Parasite Egg Count / Q000662:veterinary; D011201:Poultry Diseases / Q000276:immunology* / Q000517:prevention & control; D014611:Vaccination / Q000379:methods / Q000662:veterinary*
chemical_list: D000913:Antibodies, Protozoan; D003049:Coccidiostats