title: Anti-myelin associated glycoprotein antibodies recognize HNK-1 epitope on CNS.
abstract: Antibodies to myelin-associated glycoprotein (MAG) are associated with demyelinating polyneuropathy and are specific for the HNK-1 epitope. To test if anti-MAG IgM recognize HNK-1 on CNS, sera from 20 patients and 238 controls were tested on rat slices by indirect immunofluorescence (IIF). IgM from anti-MAG positive patients, but not from control sera, stained rat brain with perineuronal or neuropil pattern, depending on the CNS region. IIF titers significantly correlated with ELISA anti-MAG titers. The staining of patients' sera were inhibited by mouse anti-HNK-1 monoclonal antibody. Our results demonstrate that anti-MAG IgM recognizes HNK-1 outside the peripheral nerve myelin carriers.
authors.affiliation: Department of Neurological and Psychiatric Sciences, University of Firenze, Italy. masa@unifi.it
authors.forename: Sabrina
authors.initials: S
authors.lastname: Matà
authors.forename: Stefano
authors.initials: S
authors.lastname: Ambrosini
authors.forename: Tommaso
authors.initials: T
authors.lastname: Mello
authors.forename: Francesco
authors.initials: F
authors.lastname: Lolli
authors.forename: Diego
authors.initials: D
authors.lastname: Minciacchi
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D001323:Autoantibodies / Q000378:metabolism*; D001666:Binding Sites, Antibody; D001921:Brain / Q000276:immunology* / Q000378:metabolism*; D018959:CD57 Antigens / Q000276:immunology / Q000378:metabolism*; D003711:Demyelinating Diseases / Q000276:immunology / Q000378:metabolism; D000939:Epitopes / Q000276:immunology / Q000378:metabolism*; D005260:Female; D006801:Humans; D007075:Immunoglobulin M / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D018992:Myelin-Associated Glycoprotein / Q000134:cerebrospinal fluid / Q000276:immunology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D018959:CD57 Antigens; D000939:Epitopes; D007075:Immunoglobulin M; D018992:Myelin-Associated Glycoprotein