title: Discovery of the serum biomarker proteins in severe preeclampsia by proteomic analysis.
abstract: Preeclapsia (PE) is a severe disorder that occurs during pregnancy, leading to maternal and fetal morbidity and mortality. PE affects about 3-8% of all pregnancies. In this study, we conducted liquid chromatography- mass spectrometry/mass spectrometry (LC-MS/MS) to analyze serum samples depleted of the six most abundant proteins from normal and PE-affected pregnancies to profile serum proteins. A total of 237 proteins were confidently identified with <1% false discovery rate from the two groups of duplicate analysis. The expression levels of those identified proteins were compared semiquantitatively by spectral counting. To further validate the candidate proteins with a quantitative mass spectrometric method, selective reaction monitoring (SRM) and enzyme linked immune assay (ELISA) of serum samples collected from pregnant women with severe PE (n = 8) or normal pregnant women (n = 5) was conducted. α2- HS-glycoprotein (AHSG), retinol binding protein 4 (RBP4) and α-1-microglobulin/bikunin (AMBP) and Insulin like growth factor binding protein, acid labile subunit (IGFBP-ALS) were confirmed to be differentially expressed in PE using SRM (P<0.05). Among these proteins, AHSG was verified by ELISA and showed a statistically significant increase in PE samples when compared to controls.
authors.affiliation: Department of Molecular Biotechnology, Institute of Biomedical Science and Technology, Konkuk University, Seoul, Korea. kpkim@konkuk.ac.kr
authors.forename: Jisook
authors.initials: J
authors.lastname: Park
authors.forename: Dong Hyun
authors.initials: DH
authors.lastname: Cha
authors.forename: Soo Jae
authors.initials: SJ
authors.lastname: Lee
authors.forename: Young Nam
authors.initials: YN
authors.lastname: Kim
authors.forename: Young Hwan
authors.initials: YH
authors.lastname: Kim
authors.forename: Kwang Pyo
authors.initials: KP
authors.lastname: Kim
mesh_terms: D000328:Adult; D000510:Alpha-Globulins / Q000378:metabolism; D000595:Amino Acid Sequence; D015415:Biomarkers / Q000097:blood; D001798:Blood Proteins / Q000032:analysis*; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D018969:Insulin-Like Growth Factor Binding Proteins / Q000097:blood; D008969:Molecular Sequence Data; D011225:Pre-Eclampsia / Q000097:blood* / Q000175:diagnosis; D011247:Pregnancy; D020543:Proteome / Q000032:analysis*; D054839:Retinol-Binding Proteins, Plasma / Q000378:metabolism; D060749:alpha-2-HS-Glycoprotein / Q000378:metabolism
chemical_list: C556354:AHSG protein, human; D000510:Alpha-Globulins; D015415:Biomarkers; D001798:Blood Proteins; D018969:Insulin-Like Growth Factor Binding Proteins; D020543:Proteome; C101326:RBP4 protein, human; D054839:Retinol-Binding Proteins, Plasma; C016001:alpha-1-microglobulin; D060749:alpha-2-HS-Glycoprotein