title: Suitability of different media for in vitro cultivation of the ruminal protozoa species Entodinium caudatum, Eudiplodinium maggii, and Epidinium ecaudatum.
abstract: Three protozoal cultivation media were tested to determine the medium which best facilitated growth and viability of key B-type ciliates isolated from the sheep rumen. Entodinium caudatum and Eudiplodinium maggii were grown anaerobically in 50-ml flasks for 32 days in Caudatum-type (C), Kisidayova (K) or Dehority (M) medium. On day 32, in media K and M, E. caudatum cell counts were high with 5.6×10(3) and 7.8×10(3) mL(-1), respectively, and the proportion of dead cells was low with 0.6 and 1.4%, respectively. E. maggii concentrations when grown in medium M and C were 2.7×10(3) and 2.4×10(3) mL(-1), respectively, with 3.9 and 14.1% dead cells. Medium M, which favoured growth of both protozoa species, was tested again and Epidinium ecaudatum was included. Protozoa were grown for a 4-month period and samples were taken in the last two months on days 1, 7, 35 and 57. Average cell concentrations were 10.0, 0.8 and 0.5×10(3) mL(-1) for E. caudatum, E. maggii, and E. ecaudatum, respectively. In conclusion, medium M would appear to be the best choice for cultivating these three species in one medium.
authors.affiliation: ETH Zurich, Institute of Agricultural Sciences, 8092 Zurich, Switzerland. johanna.zeitz@inw.agrl.ethz.ch
authors.forename: Johanna O
authors.initials: JO
authors.lastname: Zeitz
authors.forename: Leo
authors.initials: L
authors.lastname: Meile
authors.forename: Tadeusz
authors.initials: T
authors.lastname: Michałowski
authors.forename: Michael
authors.initials: M
authors.lastname: Kreuzer
authors.forename: Carla R
authors.initials: CR
authors.lastname: Soliva
mesh_terms: D000818:Animals; D002452:Cell Count; D002470:Cell Survival; D016798:Ciliophora / Q000254:growth & development* / Q000302:isolation & purification; D003470:Culture Media / Q000737:chemistry*; D010274:Parasitology / Q000379:methods*; D012417:Rumen / Q000469:parasitology*; D012756:Sheep
chemical_list: D003470:Culture Media