title: Successive and targeted DNA integrations in the Drosophila genome by Bxb1 and phiC31 integrases.
abstract: At present ϕC31 is the only phage integrase system available for directionally regulated site-specific DNA integration in the Drosophila genome. Here we report that mycobacteriophage Bxb1 integrase also mediates targeted DNA integration in Drosophila with high specificity and efficiency. By alternately using Bxb1 and ϕC31, we were able to carry out multiple rounds of successive and targeted DNA integrations in our genomic engineering founder lines for the purpose of generating complex knock-in alleles.
authors.affiliation: Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA.
authors.forename: Juan
authors.initials: J
authors.lastname: Huang
authors.forename: Pallavi
authors.initials: P
authors.lastname: Ghosh
authors.forename: Graham F
authors.initials: GF
authors.lastname: Hatfull
authors.forename: Yang
authors.initials: Y
authors.lastname: Hong
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001287:Attachment Sites, Microbiological; D004330:Drosophila / Q000235:genetics* / Q000378:metabolism; D023061:Gene Order; D018390:Gene Targeting; D005818:Genetic Engineering; D005822:Genetic Vectors; D049750:Genome, Insect; D059765:Homologous Recombination; D019426:Integrases / Q000378:metabolism*
chemical_list: D019426:Integrases