title: Performance evaluation of Elecsys analysis system for anti-cyclic citrullinated peptide detection in comparison with commercially available ELISA assays in rheumatoid arthritis diagnosis.
abstract: OBJECTIVE
  To evaluate performance consistency of electrochemical luminescence with ELISA for anti-cyclic citrullinated peptide (CCP) antibodies detection and define their diagnostic efficacy on rheumatoid arthritis (RA).
  
  
  METHODS
  Serum anti-CCP antibodies of 144 subjects (93 RA, 17 non-RA autoimmune diseases,11 involution diseases and 23 healthy volunteers) were simultaneously determined by Elecsys assay, Immunoscan CCPlus Euro-diagnostica ELISA kit and Euroimmun anti-CCP ELISA kit.
  
  
  RESULTS
  The consistent rate and Kappa coefficients among three assays were all more than 0.97. AUCs of ROC for anti-CCP antibodies detection by Elecsys, Euro-diagnostica ELISA and Euroimmun ELISA assays were respectively 0.790, 0.773 and 0.794 (all P values=0.000), and under the optimal cutoff values their diagnostic accurate rates for RA diagnosis were 78.47%, 77.08% and 76.39% respectively.
  
  
  CONCLUSIONS
  The diagnostic performance of Elecsys assay for anti-CCP antibodies detection was satisfactory and comparable to that of classical ELISA assay. Under the same specificity Elecsys assay has a slightly but not significantly higher diagnostic accuracy and sensitivity than ELISA assay.
authors.affiliation: Department of Laboratory Medicine, West China Hospital of Sichuan University, Chengdu 610041, China.
authors.forename: Bei
authors.initials: B
authors.lastname: Cai
authors.forename: Lanlan
authors.initials: L
authors.lastname: Wang
authors.forename: Jin
authors.initials: J
authors.lastname: Liu
authors.forename: Weihua
authors.initials: W
authors.lastname: Feng
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000906:Antibodies / Q000097:blood*; D001172:Arthritis, Rheumatoid / Q000175:diagnosis*; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010456:Peptides, Cyclic / Q000097:blood / Q000276:immunology*; D012372:ROC Curve; D055815:Young Adult
chemical_list: D000906:Antibodies; D010456:Peptides, Cyclic; C487763:cyclic citrullinated peptide