title: In vitro effects of some anabolic compounds on erythrocyte carbonic anhydrase I and II.
abstract: The in vitro effects of the anabolic compounds, zeranol, 17 β-estradiol, diethylstilbestrol (DES), and trenbolone, on the activity of purified human carbonic anhydrase I and II were evaluated. In vitro CA enzyme activity was determined colorimetrically using the CO₂ hydration method of Maren. IC₅₀ values of the compounds that caused inhibition were determined by means of activity percentage diagrams. The IC₅₀ concentrations of zeranol, 17 β-estradiol, DES and trenbolone on hCA I were 94, 55, 10, 898 µM and for hCA II 89, 159, 439 and 101 μM, respectively.
authors.affiliation: Chemistry Department/Biochemistry Div., Balikesir University, Cagis Kampus, Balikesir 10145, Turkey. ngencer@balikesir.edu.tr
authors.forename: Nahit
authors.initials: N
authors.lastname: Gençer
authors.forename: Adem
authors.initials: A
authors.lastname: Ergün
authors.forename: Dudu
authors.initials: D
authors.lastname: Demir
mesh_terms: D045930:Anabolic Agents / Q000494:pharmacology*; D024401:Carbonic Anhydrase I / Q000037:antagonists & inhibitors*; D024402:Carbonic Anhydrase II / Q000037:antagonists & inhibitors*; D004054:Diethylstilbestrol / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects*; D004958:Estradiol / Q000494:pharmacology; D004967:Estrogens / Q000494:pharmacology; D004968:Estrogens, Non-Steroidal / Q000494:pharmacology; D006801:Humans; D015394:Molecular Structure; D013329:Structure-Activity Relationship; D014204:Trenbolone Acetate / Q000494:pharmacology; D015029:Zeranol / Q000494:pharmacology
chemical_list: D045930:Anabolic Agents; D004967:Estrogens; D004968:Estrogens, Non-Steroidal; D004958:Estradiol; D004054:Diethylstilbestrol; D015029:Zeranol; D024401:Carbonic Anhydrase I; D024402:Carbonic Anhydrase II; D014204:Trenbolone Acetate