title: Identification of CD8+ cytotoxic T lymphocyte epitopes from porcine reproductive and respiratory syndrome virus matrix protein in BALB/c mice.
abstract: Twenty-seven nanopeptides derived from the matrix (M) protein of porcine reproductive and respiratory syndrome virus (PRRSV) were screened for their ability to elicit a recall interferon-γ (IFN-γ) response from the splenocytes of BALB/c mice following DNA vaccination and a booster vaccination with recombinant vaccinia virus rWR-PRRSV-M. We identified two peptides (amino acid residues K93FITSRCRL and F57GYMTFVHF) as CD8+ cytotoxic T lymphocyte (CTL) epitopes. These peptides elicited significant numbers of IFN-γ secreting cells, compared with other M nonapeptides and one irrelevant nonapeptide. Bioinformatics analysis showed that the former is an H-2Kd-restricted CTL epitope, and the latter is an H-2Dd-restricted CTL epitope. Multiple amino acid sequence alignment among different PRRSV M sequences submitted to GenBank indicated that these two CTL epitopes are strongly conserved, and they should therefore be considered for further research on the mechanisms of cellular immune responses to PRRSV.
authors.affiliation: The Key Laboratory of Veterinary Public Health, Ministry of Agriculture, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, PR China.
authors.forename: Weijun
authors.initials: W
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Lin
authors.forename: Yu
authors.initials: Y
authors.lastname: Bai
authors.forename: Tiegang
authors.initials: T
authors.lastname: Tong
authors.forename: Qun
authors.initials: Q
authors.lastname: Wang
authors.forename: Nihong
authors.initials: N
authors.lastname: Liu
authors.forename: Guangliang
authors.initials: G
authors.lastname: Liu
authors.forename: Yihong
authors.initials: Y
authors.lastname: Xiao
authors.forename: Tao
authors.initials: T
authors.lastname: Yang
authors.forename: Zhigao
authors.initials: Z
authors.lastname: Bu
authors.forename: Guangzhi
authors.initials: G
authors.lastname: Tong
authors.forename: Donglai
authors.initials: D
authors.lastname: Wu
mesh_terms: D000818:Animals; D004337:Drug Carriers / Q000008:administration & dosage; D018604:Epitope Mapping; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D005260:Female; D005822:Genetic Vectors; D007371:Interferon-gamma / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D019316:Porcine respiratory and reproductive syndrome virus / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014611:Vaccination / Q000379:methods; D014616:Vaccinia virus / Q000235:genetics; D014763:Viral Matrix Proteins / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology
chemical_list: D004337:Drug Carriers; D018984:Epitopes, T-Lymphocyte; C525999:M protein, porcine reproductive and respiratory syndrome virus; D014763:Viral Matrix Proteins; D014765:Viral Vaccines; D007371:Interferon-gamma