title: A spectrophotometric assay method for vanadium in biological and environmental samples using 2,4-dinitrophenylhydrazine with imipramine hydrochloride.
abstract: A simple, rapid, and sensitive method involving the interaction of 2,4-dinitrophenylhydrazine with imipramine hydrochloride in presence of vanadium (V) in sulfuric acid medium has been proposed for the determination of vanadium. The purple-colored product developed showed an absorption maximum at 560 nm and was stable for 24 h. The working curve was linear over the concentration range of 0.1-2.8 μg ml( - 1), with sensitivity of detection of 0.0124 μg ml( - 1). Molar absorptivity and Sandell's sensitivity were found to be 2.6 × 10(4) l/mol cm and 0.0039 μg cm( - 1), respectively. The accuracy of the proposed method was assessed by Student's t test and variance ratio F test, and the results were on par with the reported method. The method was successfully used in the determination of V in water, human urine, soil, and plant samples, and it was free from interference by various concomitant ions.
authors.affiliation: Department of Studies in Chemistry, University of Mysore, Manasagangotri, Mysore 570 006, India.
authors.forename: Naef Ghllab Saeed
authors.initials: NG
authors.lastname: Al-Tayar
authors.forename: P
authors.initials: P
authors.lastname: Nagaraja
authors.forename: R A
authors.initials: RA
authors.lastname: Vasantha
authors.forename: Ashwinee Kumar
authors.initials: AK
authors.lastname: Shresta
mesh_terms: D018759:Adrenergic Uptake Inhibitors / Q000737:chemistry; D004784:Environmental Monitoring / Q000379:methods*; D005618:Fresh Water / Q000737:chemistry; D006801:Humans; D007099:Imipramine / Q000737:chemistry*; D015394:Molecular Structure; D010659:Phenylhydrazines / Q000737:chemistry*; D012987:Soil / Q000737:chemistry; D013053:Spectrophotometry / Q000379:methods*; D014556:Urine / Q000737:chemistry; D014639:Vanadium / Q000737:chemistry*
chemical_list: D018759:Adrenergic Uptake Inhibitors; D010659:Phenylhydrazines; D012987:Soil; D014639:Vanadium; C004787:2,4-dinitrophenylhydrazine; D007099:Imipramine