title: Chiral metallo-supramolecular complexes selectively induce human telomeric G-quadruplex formation under salt-deficient conditions.
abstract: Chiral molecular recognition of human telomeric DNA is important for rational drug design and developing structural probes of G-quadruplexes. Here we report that a chiral supramolecular complex can selectively induce human telomeric G-quadruplex formation and discriminate different G-quadruplex sequences under salt-deficient conditions studied by circular dichroism (CD), UV meltings, stopped-flow spectroscopy, fluorescence resonance energy transfer, enzyme cleavage, and gel electrophoresis. P-enantiomer induced G-quadruplex formation is fast and does not require a large excess of P enantiomer. More importantly, this chiral compound induces loop sequence-dependent G-quadruplex formation.
authors.affiliation: Division of Biological Inorganic Chemistry, State Key Laboratory of Rare Earth Resource Utilization, Graduate School of the Chinese Academy of Sciences, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, PR China.
authors.forename: Chuanqi
authors.initials: C
authors.lastname: Zhao
authors.forename: Jie
authors.initials: J
authors.lastname: Geng
authors.forename: Lingyan
authors.initials: L
authors.lastname: Feng
authors.forename: Jinsong
authors.initials: J
authors.lastname: Ren
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Qu
mesh_terms: D001483:Base Sequence; D002942:Circular Dichroism; D056831:Coordination Complexes / Q000737:chemistry* / Q000494:pharmacology; D004247:DNA / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer; D054856:G-Quadruplexes; D006801:Humans; D007536:Isomerism; D008024:Ligands; D009532:Nickel / Q000737:chemistry* / Q000494:pharmacology; D012492:Salts / Q000737:chemistry; D016615:Telomere / Q000187:drug effects
chemical_list: D056831:Coordination Complexes; D008024:Ligands; D012492:Salts; D009532:Nickel; D004247:DNA