title: Analysis of the amino acids of soy globulins by AOT reverse micelles and aqueous buffer.
abstract: The 7S and 11S globulins from soybean proteins using reverse micelle and aqueous buffer extraction methods were characterized by using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and scanning electron microscope (SEM), and their amino acid compositions were also evaluated. SDS-PAGE did not show electrophoretic differences between 7S and 11S globulin subunits with two extraction methods. SEM analysis showed that the AOT reverse micelle processing of 7S and 11S globulins induced a reduction of droplet size. Some individual amino acid contents of 7S and 11S globulins using two extraction methods were different, some were similar. In all the samples, the glutamic acid, aspartic acid, and leucine were the dominant amino acids while the cystine and methionine were the first-limiting amino acids. The proportion of essential amino acids to the total amino acids (E/T) of the 7S globulin from aqueous buffer and reverse micelles was similar. While significant differences were obtained in the proportion of E/T of the 11S globulin.
authors.affiliation: Institute Agro-Food Science and Technology, Shandong Academy of Agricultural Sciences, No. 198, Gongyebei Road, Jinan 250100, China. zhaoxiaoy@yahoo.cn
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Zhao
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.forename: Zhifang
authors.initials: Z
authors.lastname: Lu
authors.forename: Xiangqing
authors.initials: X
authors.lastname: Ling
authors.forename: Peng
authors.initials: P
authors.lastname: Deng
authors.forename: Qingjun
authors.initials: Q
authors.lastname: Zhu
authors.forename: Fangling
authors.initials: F
authors.lastname: Du
mesh_terms: D000596:Amino Acids / Q000032:analysis*; D002021:Buffers; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D005916:Globulins / Q000737:chemistry* / Q000378:metabolism; D008823:Micelles; D008855:Microscopy, Electron, Scanning; D052756:Nutritional Sciences; D030262:Soybean Proteins / Q000737:chemistry* / Q000378:metabolism; D013025:Glycine max / Q000737:chemistry* / Q000378:metabolism; D013386:Succinates / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry; D014867:Water
chemical_list: D000596:Amino Acids; D002021:Buffers; D005916:Globulins; D008823:Micelles; D030262:Soybean Proteins; D013386:Succinates; D013501:Surface-Active Agents; C511925:bis(2-ethylhexyl)sulfosuccinate; D014867:Water