title: Evaluation of a new DNA test for detection of carcinogenic human papillomavirus.
abstract: Using archived specimens, we evaluated a new automated real-time PCR assay (BD Diagnostics) that detects all carcinogenic human papillomaviruses (HPV) and provides HPV genotyping for seven of them, including HPV16 and HPV18, the two most carcinogenic HPV genotypes. We found comparable results with Hybrid Capture 2 (HC2) for detection of carcinogenic HPV (n = 473) and with Linear Array and Line Blot Assay (n = 371) for detection of individual HPV genotypes.
authors.affiliation: American Society for Clinical Pathology, 1225 New York Ave. NW, Suite 250, Washington, DC 20005, USA. philip.castle@ascp.org
authors.forename: Philip E
authors.initials: PE
authors.lastname: Castle
authors.forename: Erin C
authors.initials: EC
authors.lastname: Gutierrez
authors.forename: Sharon V
authors.initials: SV
authors.lastname: Leitch
authors.forename: Courtney E
authors.initials: CE
authors.lastname: Maus
authors.forename: Ray A
authors.initials: RA
authors.lastname: McMillian
authors.forename: William A
authors.initials: WA
authors.lastname: Nussbaumer
authors.forename: Laurence M
authors.initials: LM
authors.lastname: Vaughan
authors.forename: Cosette M
authors.initials: CM
authors.lastname: Wheeler
authors.forename: Patti E
authors.initials: PE
authors.lastname: Gravitt
authors.forename: Mark
authors.initials: M
authors.lastname: Schiffman
mesh_terms: D000328:Adult; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D005838:Genotype; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D030361:Papillomavirus Infections / Q000821:virology*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D014773:Virology / Q000379:methods*
chemical_list: D004279:DNA, Viral