title: Simultaneous illumination method: application in a two-channel emission fluorometer with multi-wavelength excitation.
abstract: We developed a new illumination method called the simultaneous illumination method. This method does not require synchronization between light sources and sensor signals, which drastically simplifies the instrumentation. As a proof-of-concept, we applied this method to an oceanographic fluorometer. In principle, using this method, one can easily increase the number of characterized emission wavelengths by mounting optical sensors for as many emission wavelengths as needed. Our fluorometer has two emission-wavelength channels and twelve excitation wavelengths. The aim of this prototype is to demonstrate a viable in situ N-channel emission fluorometer with multiple wavelengths of excitation, which has not been previously realized.
authors.affiliation: School of Marine Science and Technology, Tokai Univ, 3-20-1 Orido, Shimizu-Ku, Shizuoka 424-8610, Japan. ebata29@gmail.com
authors.forename: Hiroki
authors.initials: H
authors.lastname: Ebata
authors.forename: Akihiko
authors.initials: A
authors.lastname: Tanaka
authors.forename: Yohei
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Shintaro
authors.initials: S
authors.lastname: Yano
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tan
authors.forename: Roland
authors.initials: R
authors.lastname: Doerffer
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Oishi
mesh_terms: D005455:Fluorescent Antibody Technique; D008027:Light; D008029:Lighting; D009791:Oceanography / Q000379:methods*; D055095:Optics and Photonics; D010775:Photic Stimulation; D010783:Photometry; D010839:Phytoplankton / Q000378:metabolism / Q000502:physiology*; D013053:Spectrophotometry