title: Oxidation-extraction spectrometry of reactive oxygen species (ROS) generated by chlorophyllin magnesium (Chl-Mg) under ultrasonic irradiation.
abstract: In order to examine the mechanism and process of sonodynamic reaction, the chlorophyllin magnesium (Chl-Mg) acting as a sonosensitizer was irradiated by ultrasound, and the generation of reactive oxygen species (ROS) were detected by the method of oxidation-extraction spectrometry (OES). That is, under ultrasonic irradiation in the presence of Chl-Mg, the 1,5-diphenyl carbazide (DPCI) is oxidized by generated ROS into 1,5-diphenyl carbazone (DPCO), which can be extracted by mixed organic solvent and display a obvious visible absorption at 563 nm wavelength. Besides, the generation conditions of ROS were also reviewed. The results demonstrated that the quantities of generated ROS increased with the increase of ultrasonic irradiation time, Chl-Mg concentration and DPCI concentration. Finally, several radical scavengers (l-Histidine (His), 2,6-Di-tert-butyl-methylphenol (BHT) and Vitamin C (VC)) were used to determine the kind of the generated ROS. It was found that at least the hydroxyl radical (OH) and singlet oxygen (1O2) were generated in the presence of Chl-Mg under ultrasonic irradiation. It is wish that this paper might offer some valuable references for the study on the mechanism of SDT and the application of Chl-Mg in tumor treatment.
authors.affiliation: College of Chemistry, Liaoning University, Shenyang 110036, PR China.
authors.forename: Yuwei
authors.initials: Y
authors.lastname: Guo
authors.forename: Chunping
authors.initials: C
authors.lastname: Cheng
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Xudong
authors.initials: X
authors.lastname: Jin
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.forename: Zhiqiu
authors.initials: Z
authors.lastname: Wang
authors.forename: Jingqun
authors.initials: J
authors.lastname: Gao
authors.forename: Pingli
authors.initials: P
authors.lastname: Kang
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D001205:Ascorbic Acid / Q000737:chemistry / Q000494:pharmacology; D002084:Butylated Hydroxytoluene; D002735:Chlorophyllides / Q000737:chemistry / Q000528:radiation effects*; D016166:Free Radical Scavengers / Q000494:pharmacology; D006639:Histidine / Q000737:chemistry; D017665:Hydroxyl Radical; D008274:Magnesium / Q000737:chemistry / Q000528:radiation effects*; D010084:Oxidation-Reduction; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000032:analysis* / Q000528:radiation effects*; D013057:Spectrum Analysis; D014465:Ultrasonics
chemical_list: D000975:Antioxidants; D002735:Chlorophyllides; D016166:Free Radical Scavengers; D010636:Phenols; D017382:Reactive Oxygen Species; D002084:Butylated Hydroxytoluene; D017665:Hydroxyl Radical; D006639:Histidine; C007020:chlorophyllin; D008274:Magnesium; D001205:Ascorbic Acid