title: Molecular characterization, expression and functional analysis of a nuclear oligomerization domain proteins subfamily C (NLRC) in Japanese flounder (Paralichthys olivaceus).
abstract: Pattern recognition receptors (PRRs) are involved in the effective innate defense against several microbes. Here, we identified a nucleotide-oligomerization domain (NOD)-like receptor subfamily C (NLRC) from Japanese flounder (Paralichthys olivaceus). Full-length transcript of JfNLRC is composed of 3976 bp encoding a protein of 1175 deduced amino acid residues. The presence of a signature nucleotide-binding domain (NACHT) and leucine-rich repeated domain (LRR) suggested that the protein is a member of the NLR family. Interestingly, its C-terminus presents an extra PRY/SPRY (B30.2) domain similar to fish in the Trim (finTrim) family. A phylogenic tree of JfNLRC revealed that full-length JfNLRC diverged from the NOD1 and NOD2 clusters, and the NACHT domain in JfNLRC was clustered within the NLRC3 group. Stimulation by formalin-killed Edwardsiella tarda, Streptococcus iniae, and lipopolysaccharide (LPS) showed that the JfNLRC expression was raised a few hours after stimulation, suggesting this novel protein is involved in the immediate response against both Gram-positive and Gram-negative bacteria. Furthermore, the IL-1β mRNA expression level in JfNLRC-over-expressing HINAE cells was significantly increased, when compared to a control, after LPS-stimulation and E. tarda infection. These results suggested that JfNLRC probably induced IL-1β gene expression mediated by LPS-stimulation.
authors.affiliation: Laboratory of Genome Science, Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato, Tokyo 108-8477, Japan.
authors.forename: Sasimanas
authors.initials: S
authors.lastname: Unajak
authors.forename: Mudjekeewis D
authors.initials: MD
authors.lastname: Santos
authors.forename: Jun-Ichi
authors.initials: J
authors.lastname: Hikima
authors.forename: Tae-Sung
authors.initials: TS
authors.lastname: Jung
authors.forename: Hidehiro
authors.initials: H
authors.lastname: Kondo
authors.forename: Ikuo
authors.initials: I
authors.lastname: Hirono
authors.forename: Takashi
authors.initials: T
authors.lastname: Aoki
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D020609:Edwardsiella tarda; D004756:Enterobacteriaceae Infections / Q000276:immunology / Q000662:veterinary; D005393:Fish Diseases / Q000276:immunology; D029941:Fish Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D005432:Flounder / Q000235:genetics / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D008070:Lipopolysaccharides / Q000276:immunology; D008969:Molecular Sequence Data; D053472:Nod Signaling Adaptor Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D010802:Phylogeny; D017385:Sequence Homology; D013290:Streptococcal Infections / Q000276:immunology / Q000662:veterinary; D013291:Streptococcus / Q000276:immunology
chemical_list: D029941:Fish Proteins; D008070:Lipopolysaccharides; D053472:Nod Signaling Adaptor Proteins