title: Ontogeny of salinity tolerance and evidence for seawater-entry preparation in juvenile green sturgeon, Acipenser medirostris.
abstract: We measured the ontogeny of salinity tolerance and the preparatory hypo-osmoregulatory physiological changes for seawater entry in green sturgeon (Acipenser medirostris), an anadromous species occurring along the Pacific Coast of North America. Salinity tolerance was measured every 2 weeks starting in 40-day post-hatch (dph) juveniles and was repeated until 100% survival at 34‰ was achieved. Fish were subjected to step increases in salinity (5‰ 12 h(-1)) that culminated in a 72-h exposure to a target salinity, and treatment groups (0, 15, 20, 25, 30, 34‰; and abrupt exposure to 34‰) were adjusted as fish developed. After 100% survival was achieved (134 dph), a second experiment tested two sizes of fish for 28-day seawater (33‰) tolerance, and gill and gastrointestinal tract tissues were sampled. Their salinity tolerance increased and plasma osmolality decreased with increasing size and age, and electron microscopy revealed three types of mitochondria-rich cells: one in fresh water and two in seawater. In addition, fish held on a natural photoperiod in fresh water at 19°C showed peaks in cortisol, thyroid hormones and gill and pyloric ceca Na(+), K(+)-ATPase activities at body sizes associated with seawater tolerance. Therefore, salinity tolerance in green sturgeon increases during ontogeny (e.g., as these juveniles may move down estuaries to the ocean) with increases in body size. Also, physiological and morphological changes associated with seawater readiness increased in freshwater-reared juveniles and peaked at their seawater-tolerant ages and body sizes. Their seawater-ready body size also matched that described for swimming performance decreases, presumably associated with downstream movements. Therefore, juvenile green sturgeon develop structures and physiological changes appropriate for seawater entry while growing in fresh water, indicating that hypo-osmoregulatory changes may proceed by multiple routes in sturgeons.
authors.affiliation: Department of Wildlife, Fish, and Conservation Biology, University of California, Davis, CA 95616, USA. pallen@cfr.msstate.edu
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Allen
authors.forename: Maryann
authors.initials: M
authors.lastname: McEnroe
authors.forename: Tetyana
authors.initials: T
authors.lastname: Forostyan
authors.forename: Stephanie
authors.initials: S
authors.lastname: Cole
authors.forename: Mary M
authors.initials: MM
authors.lastname: Nicholl
authors.forename: Brian
authors.initials: B
authors.lastname: Hodge
authors.forename: Joseph J
authors.initials: JJ
authors.lastname: Cech
mesh_terms: D000367:Age Factors; D000825:Animal Structures / Q000201:enzymology; D000818:Animals; D049628:Body Size / Q000502:physiology; D002712:Chlorides / Q000097:blood; D004847:Epithelial Cells / Q000648:ultrastructure; D005399:Fishes / Q000502:physiology*; D005618:Fresh Water; D005880:Gills / Q000166:cytology / Q000201:enzymology; D048788:Growth and Development / Q000502:physiology*; D006400:Hematocrit; D006854:Hydrocortisone / Q000097:blood; D007422:Intestines / Q000201:enzymology; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D008928:Mitochondria / Q000648:ultrastructure; D009994:Osmolar Concentration; D010949:Plasma / Q000737:chemistry; D054712:Salinity; D055049:Salt Tolerance / Q000502:physiology*; D012623:Seawater; D012964:Sodium / Q000097:blood; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D015996:Survival Rate; D013974:Thyroxine / Q000097:blood; D014284:Triiodothyronine / Q000097:blood
chemical_list: D002712:Chlorides; D014284:Triiodothyronine; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase; D013974:Thyroxine; D006854:Hydrocortisone