title: [Biomechanical study on decellularized laryngeal scaffold in dogs].
abstract: OBJECTIVE
  To evaluate the biomechanical characteristics of the decellularized laryngeal scaffold.
  
  
  METHODS
  Ten Chinese adult dogs were randomly divided into two groups: perfusion group (n = 5) and control group (n = 5). The acellular larynx scaffold was obtained from dogs through cranial thyroid arteries perfusion with detergents. Comparative examinations were performed by the macroscopic view, histological view (hematoxylin and eosin stain, Alcian blue stain and Masson stain), scanning electron microscope (SEM) and biomechanical properties between perfusion group and control group.
  
  
  RESULTS
  Macroscopic view showed that the decellularized laryngeal scaffold appeared pale asphyxia. HE stain indicated that there were little acellular traces of muscle and mucosa. Alcian blue stain, Masson stain and scanning electron microscope (SEM) suggested that there were no obvious changes about glycosaminoglycan and collagen. The compressive modulus of thyroid cartilage was (1.06 ± 0.07) MPa (x(-) ± s) in experimental groups and (1.15 ± 0.11) MPa in control group, showing no significant difference (t = 1.424, P > 0.05), neither in compressive modulus of annular cartilage (1.68 ± 0.11) MPa in experimental groups and (1.67 ± 0.09) MPa in control group (t = 0.185, P > 0.05). The tensile strength of thyroid cartilage between experimental (5.74 ± 0.88) MPa and control groups (6.18 ± 1.33) MPa did not have the statistical significance (t = 0.627, P > 0.05).
  
  
  CONCLUSIONS
  These results indicate that perfusion method can construct a perfect biomechanical acellular larynx scaffold which could be a better selection for laryngeal reconstruction with tissue engineering method.
authors.affiliation: Department of Otorhinolaryngology, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
authors.forename: Li
authors.initials: L
authors.lastname: Xu
authors.forename: Peng-cheng
authors.initials: PC
authors.lastname: Cui
authors.forename: Zhi-feng
authors.initials: ZF
authors.lastname: Chen
authors.forename: Rui-na
authors.initials: RN
authors.lastname: Ma
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D002358:Cartilage, Articular; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D004285:Dogs; D007831:Larynx, Artificial; D015607:Stents; D013718:Tensile Strength; D023822:Tissue Engineering; D054457:Tissue Scaffolds