title: Application of Fluolid-Orange-labeled probes for DNA microarray and immunological assays.
abstract: The usefulness of Fluolid-Orange, a novel fluorescent dye, for DNA microarray and immunological assays has been examined. Fluolid-Orange-labeled probes (DNA and IgG) were stable as examined by laser-photo-bleaching and under heat and dry conditions. Statistical analyses were performed to evaluate the reproducibility of the microarray assay, while stage-specific immunostaining of marker proteins, Kank1 and calretinin, was performed for renal cancers, both giving satisfactory results. The stability of the dye should provide advantages for storing fluorescently labeled probes and re-examining the specimens later in genetic and pathological diagnostics.
authors.affiliation: Signaling Molecules Research Group, Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhu
authors.forename: Takunori
authors.initials: T
authors.lastname: Ogaeri
authors.forename: Jun-ichiro
authors.initials: J
authors.lastname: Suzuki
authors.forename: Sijun
authors.initials: S
authors.lastname: Dong
authors.forename: Tei-ichiro
authors.initials: T
authors.lastname: Aoyagi
authors.forename: Keiji
authors.initials: K
authors.lastname: Mizuki
authors.forename: Mikako
authors.initials: M
authors.lastname: Takasugi
authors.forename: Shin-ichiro
authors.initials: S
authors.lastname: Isobe
authors.forename: Ryoiti
authors.initials: R
authors.lastname: Kiyama
mesh_terms: D004247:DNA / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007074:Immunoglobulin G / Q000737:chemistry; D015335:Molecular Probes / Q000737:chemistry; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D057089:Pathology, Molecular / Q000379:methods*; D015203:Reproducibility of Results; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D007074:Immunoglobulin G; D015335:Molecular Probes; D004247:DNA