title: Induced pluripotent stem cells' self-renewal and pluripotency is maintained by a bovine granulosa cell line-conditioned medium.
abstract: Induced pluripotent stem cells (iPSCs) are a promising type of stem cells, comparable to embryonic stem cells (ESCs) in terms of self-renew and pluripotency, generated by reprogramming somatic cells. These cells are an attractive approach to supply patient-specific pluripotent cells, for producing in vitro models of disease, drug discovery, toxicology and potentially treating degenerative disease circumventing immune rejection. In spite of the great advance since iPSCs' establishment, their obtention and propagation is an increasing area of great interest. In a recent work, we have shown that the conditioned medium from a bovine granulosa cell line (BGC-CM) is able to preserve the basic properties of mESCs. Therefore, based on our previous results and the reported resemblance between iPSCs and ESCs, we hypothesized that BGC-CM could provide a favorable context to culturing iPSCs. In this work, we have reprogrammed mouse embryonic fibroblasts obtaining iPSC lines, and showed that they can be propagated in BGC-CM while maintaining self-renewal and pluripotency, evidenced by expression of specific gene markers and capability of in vitro and in vivo differentiation to cell types from the three germ layers. We believe that these findings may provide a novel context to propagate iPSCs to study the molecular mechanisms involved in self-renewal and pluripotency.
authors.affiliation: Laboratorio de Regulación de Expresión Génica en el Crecimiento, Supervivencia y Diferenciación Celular, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina. cmsolari@qb.fcen.uba.ar
authors.forename: Claudia
authors.initials: C
authors.lastname: Solari
authors.forename: Noelia
authors.initials: N
authors.lastname: Losino
authors.forename: Carlos
authors.initials: C
authors.lastname: Luzzani
authors.forename: Ariel
authors.initials: A
authors.lastname: Waisman
authors.forename: Carolina
authors.initials: C
authors.lastname: Bluguermann
authors.forename: Maria
authors.initials: M
authors.lastname: Questa
authors.forename: Gustavo
authors.initials: G
authors.lastname: Sevlever
authors.forename: Santiago
authors.initials: S
authors.lastname: Miriuka
authors.forename: Lino
authors.initials: L
authors.lastname: Barañao
authors.forename: Alejandra
authors.initials: A
authors.lastname: Guberman
mesh_terms: D000818:Animals; D002417:Cattle; D018929:Cell Culture Techniques; D002460:Cell Line; D017077:Culture Media, Conditioned / Q000378:metabolism / Q000494:pharmacology*; D005260:Female; D006107:Granulosa Cells / Q000378:metabolism / Q000502:physiology; D057026:Induced Pluripotent Stem Cells / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D050814:Octamer Transcription Factor-3 / Q000378:metabolism; D012038:Regeneration
chemical_list: D017077:Culture Media, Conditioned; D050814:Octamer Transcription Factor-3