title: Collagen XXIII, novel ligand for integrin alpha2beta1 in the epidermis.
abstract: Cellular receptors for collagens belong to the family of β(1) integrins. In the epidermis, integrin α(2)β(1) is the only collagen-binding integrin present. Its expression is restricted to basal keratinocytes with uniform distribution on the cell surface of those cells. Although α(2)β(1) receptors localized at the basal surface interact with basement membrane proteins collagen IV and laminin 111 and 332, no interaction partners have been reported for these integrin molecules at the lateral and apical membranes of basal keratinocytes. Solid phase binding and surface plasmon resonance spectroscopy demonstrate that collagen XXIII, a member of the transmembrane collagens, directly interacts with integrin α(2)β(1) in an ion- and conformation-dependent manner. The two proteins co-localize on the surface of basal keratinocytes. Furthermore, collagen XXIII is sufficient to induce adhesion and spreading of keratinocytes, a process that is significantly reduced in the absence of functional integrin α(2)β(1).
authors.affiliation: Center for Biochemistry, Medical Faculty, University of Cologne, D-50931 Cologne, Germany.
authors.forename: Guido
authors.initials: G
authors.lastname: Veit
authors.forename: Daniela
authors.initials: D
authors.lastname: Zwolanek
authors.forename: Beate
authors.initials: B
authors.lastname: Eckes
authors.forename: Stephan
authors.initials: S
authors.lastname: Niland
authors.forename: Jarmo
authors.initials: J
authors.lastname: Käpylä
authors.forename: Manon C
authors.initials: MC
authors.lastname: Zweers
authors.forename: Akemi
authors.initials: A
authors.lastname: Ishada-Yamamoto
authors.forename: Thomas
authors.initials: T
authors.lastname: Krieg
authors.forename: Jyrki
authors.initials: J
authors.lastname: Heino
authors.forename: Johannes A
authors.initials: JA
authors.lastname: Eble
authors.forename: Manuel
authors.initials: M
authors.lastname: Koch
mesh_terms: D002448:Cell Adhesion; D002460:Cell Line; D003094:Collagen / Q000378:metabolism*; D004817:Epidermis / Q000378:metabolism*; D022001:Focal Adhesions; D006801:Humans; D007150:Immunohistochemistry; D038982:Integrin alpha2beta1 / Q000378:metabolism*; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism; D008024:Ligands; D020349:Surface Plasmon Resonance
chemical_list: D038982:Integrin alpha2beta1; D008024:Ligands; D003094:Collagen