title: A common variant in the adiponectin gene and polycystic ovary syndrome risk.
abstract: In this study, we explored whether polymorphisms in insulin receptor (INSR), adiponectin (ADIPOQ), parathyroid hormone (PTH), and vitamin D receptor (VDR) genes are associated with polycystic ovary syndrome (PCOS). A total of 362 subjects, including 181 women with PCOS and 181 controls were enrolled in this case-control study. Two SNPs (rs2059806 and rs1799817) in the INSR gene, two SNPs (rs2241766 and rs1501299) in the ADIPOQ gene, one SNP (rs6256) in the PTH gene, and one SNP (rs757343) in the VDR gene were analyzed using PCR-RFLP method. We observed no significant difference in genotype and allele frequencies between the women with PCOS and controls for the rs2059806, rs1799817, rs1501299, rs6256, and rs757343 polymorphisms either before or after adjustment for confounding factors including age and BMI. However, the ADIPOQ rs2241766 "TT" genotype compared with "TG and GG" genotypes was associated with a 1.93-fold increased risk for PCOS (P = 0.006, OR = 1.93, 95% CI = 1.20-3.11), and the differences remained significant after adjustment for age and BMI (P = 0.039, OR = 1.72, 95% CI = 1.03-2.86). Furthermore, the ADIPOQ rs2241766 "T" allele was significantly overrepresented in women with PCOS than controls (P = 0.006; OR = 1.80, 95% CI = 1.18-2.70), and the difference remained significant after Bonferroni correction. Our findings suggest that the ADIPOQ rs2241766 "TT" genotype is a marker of increased PCOS susceptibility. This study also indicates for the first time that there are no significant association between INSR rs2059806, PTH rs6256, and VDR rs757343 gene polymorphisms and PCOS risk. However, these data remain to be confirmed in larger studies and in other populations.
authors.affiliation: Research Center for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Fariba
authors.initials: F
authors.lastname: Ranjzad
authors.forename: Touraj
authors.initials: T
authors.lastname: Mahmoudi
authors.forename: Atena
authors.initials: A
authors.lastname: Irani Shemirani
authors.forename: Aidin
authors.initials: A
authors.lastname: Mahban
authors.forename: Abdolrahim
authors.initials: A
authors.lastname: Nikzamir
authors.forename: Mohsen
authors.initials: M
authors.lastname: Vahedi
authors.forename: Mahnaz
authors.initials: M
authors.lastname: Ashrafi
authors.forename: Hamid
authors.initials: H
authors.lastname: Gourabi
mesh_terms: D052242:Adiponectin / Q000235:genetics*; D000367:Age Factors; D015992:Body Mass Index; D017931:DNA Primers / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D016015:Logistic Models; D016017:Odds Ratio; D010281:Parathyroid Hormone / Q000235:genetics; D011085:Polycystic Ovary Syndrome / Q000453:epidemiology* / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011972:Receptor, Insulin / Q000235:genetics; D018167:Receptors, Calcitriol / Q000235:genetics; D012307:Risk Factors
chemical_list: C499918:ADIPOQ protein, human; D052242:Adiponectin; D017931:DNA Primers; D010281:Parathyroid Hormone; D018167:Receptors, Calcitriol; D011972:Receptor, Insulin