title: The interplay between surfaces and soluble factors define the immunologic and angiogenic properties of myeloid dendritic cells.
abstract: BACKGROUND
  Dendritic cells (DCs) are antigen presenting cells capable of inducing specific immune responses against microbial infections, transplant antigens, or tumors. Interestingly, microenvironment conditions such as those present in tumor settings might induce a DC phenotype that is poorly immunogenic and with the capability of promoting angiogenesis. We hypothesize that this plasticity may be caused not only by the action of specific cytokines or growth factors but also by the properties of the surfaces with which they interact, such as extracellular matrix (ECM) components.
  
  
  RESULTS
  Herewith we studied the effect of different surfaces and soluble factors on the biology of DCs. To accomplish this, we cultured murine myeloid(m) DCs on surfaces coated with fibronectin, collagen I, gelatin, and Matrigel using poly-D-lysine and polystyrene as non-biological surfaces. Further, we cultured these cells in the presence of regular DC medium (RPMI 10% FBS) or commercially available endothelial medium (EGM-2). We determined that mDCs could be kept in culture up to 3 weeks in these conditions, but only in the presence of GM-CSF. We were able to determine that long-term DC cultures produce an array of angiogenic factors, and that some of these cultures still retain the capability to induce T cell responses.
  
  
  CONCLUSIONS
  Altogether these data indicate that in order to design DC-based vaccines or treatments focused on changing the phenotype of DCs associated with diseases such as cancer or atherosclerosis, it becomes necessary to fully investigate the microenvironment in which these cells are present or will be delivered.
authors.affiliation: Biomedical Engineering Program, Russ College of Engineering and Technology, Ohio University, USA.
authors.forename: Leslee
authors.initials: L
authors.lastname: Sprague
authors.forename: Maria
authors.initials: M
authors.lastname: Muccioli
authors.forename: Michelle
authors.initials: M
authors.lastname: Pate
authors.forename: Evan
authors.initials: E
authors.lastname: Meles
authors.forename: John
authors.initials: J
authors.lastname: McGinty
authors.forename: Harika
authors.initials: H
authors.lastname: Nandigam
authors.forename: Amritha K
authors.initials: AK
authors.lastname: Venkatesh
authors.forename: Ming-Yu
authors.initials: MY
authors.lastname: Gu
authors.forename: Kristen
authors.initials: K
authors.lastname: Mansfield
authors.forename: Andrew
authors.initials: A
authors.lastname: Rutowski
authors.forename: Omowaleola
authors.initials: O
authors.lastname: Omosebi
authors.forename: Maria C
authors.initials: MC
authors.lastname: Courreges
authors.forename: Fabian
authors.initials: F
authors.lastname: Benencia
mesh_terms: D042501:Angiogenic Proteins / Q000378:metabolism; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D045744:Cell Line, Tumor; D003094:Collagen; D024042:Collagen Type I / Q000494:pharmacology; D003713:Dendritic Cells / Q000187:drug effects / Q000502:physiology*; D004338:Drug Combinations; D005260:Female; D005353:Fibronectins / Q000494:pharmacology; D005780:Gelatin / Q000494:pharmacology; D007797:Laminin; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008810:Mice, Inbred C57BL; D022423:Myeloid Cells / Q000187:drug effects / Q000502:physiology*; D011107:Polylysine; D011137:Polystyrenes; D011509:Proteoglycans; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D042501:Angiogenic Proteins; D024042:Collagen Type I; D004338:Drug Combinations; D005353:Fibronectins; D007797:Laminin; D011137:Polystyrenes; D011509:Proteoglycans; C059485:matrigel; D011107:Polylysine; D005780:Gelatin; D003094:Collagen