title: Oxidative lipid, protein, and DNA damage as oxidative stress markers in vascular complications of diabetes mellitus.
abstract: OBJECTIVE
  The purpose of this study was to determine the effects of diabetic complications on oxidation of proteins, lipids, and DNA and to investigate the relationship between oxidative damage markers and clinical parameters.
  
  
  METHODS
  The study group consisted of 69 type 2 diabetic patients (20 patients without complication, 49 patients with complication) who attended internal medicine outpatient clinics of Istanbul Education and Research Hospital and 19 healthy control subjects. In serum samples of both diabetic patients and healthy subjects, 8-hydroxy-2'deoxyguanosine (8-OHdG), as a marker of oxidative DNA damage, N(ε)-(hexanoyl)lysine (HEL) and 15-F2t-iso-prostaglandin (15-F2t-IsoP). as products of lipooxidative damage, advanced oxidation protein products (AOPP), as markers of protein damage, and paraoxonase1 (PON1) as antioxidant were studied.
  
  
  RESULTS
  15-F2t-IsoP (p < 0.005) and AOPP (p < 0.001) levels were significantly higher in diabetic group than control group while there were no significant differences in levels of 8-OHdG and HEL between the two groups. AOPP (p < 0.001) and 8-OHdG (p < 0.001) were significantly higher in diabetic group with complications compared to diabetic group without complications.
  
  
  CONCLUSIONS
  Increased formation of free radicals and oxidative stress, under conditions of hyperglycaemia, is one of the probable causes for evolution of complications in diabetes mellitus. Our study supports the hypothesis that oxidant/antioxidant balance is disturbed in diabetic patients.
authors.affiliation: Istanbul Education and Research Hospital, Internal Medicine Clinic, Istanbul, Turkey.
authors.forename: Omur
authors.initials: O
authors.lastname: Tabak
authors.forename: Remise
authors.initials: R
authors.lastname: Gelisgen
authors.forename: Hayriye
authors.initials: H
authors.lastname: Erman
authors.forename: Fusun
authors.initials: F
authors.lastname: Erdenen
authors.forename: Cüneyt
authors.initials: C
authors.lastname: Muderrisoglu
authors.forename: Hale
authors.initials: H
authors.lastname: Aral
authors.forename: Hafize
authors.initials: H
authors.lastname: Uzun
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D043303:Aryldialkylphosphatase / Q000097:blood / Q000378:metabolism; D004249:DNA Damage / Q000502:physiology*; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000097:blood; D003920:Diabetes Mellitus / Q000097:blood* / Q000378:metabolism* / Q000503:physiopathology; D015237:Dinoprost / Q000031:analogs & derivatives; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D028421:Isoprostanes / Q000097:blood*; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000502:physiology*; D011506:Proteins / Q000378:metabolism*
chemical_list: D028421:Isoprostanes; D011506:Proteins; C075750:8-epi-prostaglandin F2alpha; D000080242:8-Hydroxy-2'-Deoxyguanosine; D015237:Dinoprost; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human; D003849:Deoxyguanosine