title: Characterisation of a glycosylated alkyl polyglycoside produced by a cyclodextrin glycosyltransferase by HPLC-ELSD and -MS.
abstract: A transglycosylation reaction between an alkyl polyglycoside and α-cyclodextrin catalysed by cyclodextrin glycosyltransferase (CGTase) from Bacillus macerans was investigated. The reaction products were identified by comparison with standards generated by CGTase catalysed modification of pure alkyl glycosides using HPLC-ELSD and -MS analysis. The main products were alkyl glucopyranosides (substrates present in the alkyl polyglycoside) glycosylated with 6 (primary coupling products) or 12 (secondary coupling products) glucose residues. Both α and β anomers were glycosylated.
authors.affiliation: Department of Biotechnology, Lund University, Lund, Sweden.
authors.forename: David
authors.initials: D
authors.lastname: Svensson
authors.forename: Patrick
authors.initials: P
authors.lastname: Adlercreutz
mesh_terms: D001407:Bacillus / Q000201:enzymology; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005964:Glucosyltransferases / Q000378:metabolism*; D006027:Glycosides / Q000737:chemistry / Q000378:metabolism*; D006031:Glycosylation; D008027:Light; D013058:Mass Spectrometry / Q000379:methods*; D012542:Scattering, Radiation; D047391:alpha-Cyclodextrins / Q000378:metabolism
chemical_list: D006027:Glycosides; D047391:alpha-Cyclodextrins; D005964:Glucosyltransferases; C019963:cyclomaltodextrin glucanotransferase; C032613:alpha-cyclodextrin