title: Magnetic purification of plasminogen from human plasma by specific lysine affinity.
abstract: A novel magnetic adsorbent was prepared by covalently binding lysine onto the surface of nanoparticles via a carbodiimide coupling method. The adsorption of plasminogen onto surface-modified magnetic nanoparticles from human plasma was studied in a batch system. Surface modifications of particles were characterized using Fourier-transformed infrared spectra, transmission electron micrography, and ninhydrin assay. The maximum weight ratio of lysine to the superparamagnetic particles was 30 μmol/mg of particles. Effects of pH and temperature on plasminogen adsorption by the magnetic particles were evaluated. Desorption of plasminogen from the magnetic adsorbent was investigated using aminocaproic acid, a lysine analogue. Using a buffer composed by aminocaproic acid or lysine, plasminogen on the magnetic nanoparticles could be eluted. Overall, the results demonstrated that the lysine-coated magnetic adsorbent increased the efficiency and speed of recovery of plasminogen from human plasma.
authors.affiliation: Graduate Institute of Biochemical and Biomedical Engineering, Chang Gung University, 259 Wen-Hwa first Road, Kwei-Shan, Tao-Yuan 333, Taiwan. chl@mail.cgu.edu.tw
authors.forename: Chi-Hsien
authors.initials: CH
authors.lastname: Liu
authors.forename: Wei-Chi
authors.initials: WC
authors.lastname: Wu
authors.forename: Hsuan-Yu
authors.initials: HY
authors.lastname: Lai
authors.forename: Hau-Yaun
authors.initials: HY
authors.lastname: Hou
mesh_terms: D000327:Adsorption; D001774:Blood Chemical Analysis; D006801:Humans; D007700:Kinetics; D008239:Lysine / Q000031:analogs & derivatives / Q000378:metabolism*; D008280:Magnetics / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D010958:Plasminogen / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D013499:Surface Properties; D013696:Temperature
chemical_list: D010958:Plasminogen; D008239:Lysine