title: CARD-FISH and confocal laser scanner microscopy to assess successional changes of the bacterial community in freshwater biofilms.
abstract: Bacterial community composition was assessed during riverine biofilm development by the Catalyzed Reporter Deposition Fluorescence In Situ Hybridization (CARD-FISH) in combination with Confocal Laser Scanning Microscopy. Using artificial substrates, it was possible to follow the dynamics of specific bacterial clusters, while maintaining the unaltered structure and architecture of the biofilm.
authors.affiliation: Water Research Institute, IRSA-CNR, via Salaria km 29.300, 00015 Monterotondo, Roma, Italy.
authors.forename: Giuliano
authors.initials: G
authors.lastname: Lupini
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Proia
authors.forename: Mariarita
authors.initials: M
authors.lastname: Di Maio
authors.forename: Stefano
authors.initials: S
authors.lastname: Amalfitano
authors.forename: Stefano
authors.initials: S
authors.lastname: Fazi
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics* / Q000254:growth & development; D001431:Bacteriological Techniques / Q000379:methods; D044822:Biodiversity; D018441:Biofilms / Q000254:growth & development*; D005618:Fresh Water / Q000382:microbiology*; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D018613:Microscopy, Confocal / Q000379:methods*