title: [Identification of protein markers for serum diagnosis of cancer based on microRNA expression profiling].
abstract: A new algorithm has been developed for bioinformatics search of putative serum markers of cancer, which includes: 1) identification of microRNAs that are most often and most significantly overexpressed in tumors; 2) selection of mRNA targets regulated by microRNAs; 3) identification of mRNA targets encoding secreted proteins; 4) comparative analysis of mRNA transcription levels in normal and tumor tissues. Application of the algorithm led to discovery of seven putative serum markers of colon cancer: ADAMTS14, ANGPT2, CCL7, DEFA5, MMP11, MMP14, and PLAU. Experiments demonstrated that production of two out of seven proteins (MMP14 and DEFA5) is significantly increased in colon tumors vs. normal samples.
authors.forename: Iu A
authors.initials: IuA
authors.lastname: Bukurova
authors.forename: S L
authors.initials: SL
authors.lastname: Nikitina
authors.forename: S L
authors.initials: SL
authors.lastname: Khankin
authors.forename: G S
authors.initials: GS
authors.lastname: Krasnov
authors.forename: N A
authors.initials: NA
authors.lastname: Lisitsin
authors.forename: V L
authors.initials: VL
authors.lastname: Karpov
authors.forename: S F
authors.initials: SF
authors.lastname: Beresten'
mesh_terms: D000465:Algorithms; D014408:Biomarkers, Tumor / Q000097:blood* / Q000235:genetics; D019295:Computational Biology / Q000379:methods*; D030541:Databases, Genetic; D023082:Defensins / Q000097:blood / Q000235:genetics; D020869:Gene Expression Profiling; D006801:Humans; D053511:Matrix Metalloproteinase 14 / Q000097:blood / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics*; D009369:Neoplasms / Q000097:blood* / Q000175:diagnosis
chemical_list: D014408:Biomarkers, Tumor; D023082:Defensins; D035683:MicroRNAs; C507086:MMP14 protein, human; D053511:Matrix Metalloproteinase 14