title: Sperm acrosin is responsible for the sperm binding to the egg envelope during fertilization in Japanese quail (Coturnix japonica).
abstract: An antibody library against quail sperm plasma membrane components was established and a mAb, which strongly inhibits sperm perforations of the perivitelline membrane (PVM) was obtained from the library. The antigen molecule of the mAb showed an apparent molecular weight of 45  kDa, and was distributed both on the surface and in the acrosomal matrix of the sperm head. Periodate oxidation revealed that the epitope of the antigen includes a sugar moiety. Tandem mass spectrometry analysis of the antigen revealed that the mAb recognizes sperm acrosin. When sodium dodecyl sulfate-solubilized PVM immobilized on a polyvinylidene difluoride membrane was incubated with sperm plasma membrane lysates, the sperm acrosin was detected on the PVM immobilized on the membrane, indicating that the sperm acrosin interacts with the components of PVM. Indeed, the mAb effectively inhibited the binding of acrosome-intact sperm to the PVM. These results indicate that the 45  kDa sperm acrosin is involved in the binding of sperm to the PVM in fertilization of Japanese quail.
authors.affiliation: Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, 836 Ohya, Shizuoka 422-8529, Japan. atsasan@ipc.shizuoka.ac.jp
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Sasanami
authors.forename: Norio
authors.initials: N
authors.lastname: Yoshizaki
authors.forename: Hideo
authors.initials: H
authors.lastname: Dohra
authors.forename: Hideo
authors.initials: H
authors.lastname: Kubo
mesh_terms: D000176:Acrosin / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000502:physiology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D000918:Antibody Specificity; D030161:Avian Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000502:physiology*; D015153:Blotting, Western; D002462:Cell Membrane; D003370:Coturnix / Q000502:physiology*; D015180:Electrophoresis, Gel, Two-Dimensional; D018604:Epitope Mapping; D005260:Female; D005307:Fertilization in Vitro; D008297:Male; D008970:Molecular Weight; D021241:Spectrometry, Mass, Electrospray Ionization; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000166:cytology / Q000502:physiology*; D053719:Tandem Mass Spectrometry; D015044:Zona Pellucida / Q000502:physiology*
chemical_list: D000911:Antibodies, Monoclonal; D030161:Avian Proteins; D000176:Acrosin