title: Type-2 histone deacetylases as new regulators of elicitor-induced cell death in plants.
abstract: • Plant resistance to pathogen attack is often associated with a localized programmed cell death called hypersensitive response (HR). How this cell death is controlled remains largely unknown. • Upon treatment with cryptogein, an elicitor of tobacco defence and cell death, we identified NtHD2a and NtHD2b, two redundant isoforms of type-2 nuclear histone deacetylases (HDACs). These HDACs are phosphorylated after a few minutes' treatment, and their rate of mRNAs are rapidly and strongly reduced, leading to a 40-fold decrease after 10 h of treatment. • By using HDAC inhibitors, RNAi- and overexpression-based approaches, we showed that HDACs, and especially NtHD2a/b, act as inhibitors of cryptogein-induced cell death. Moreover, in NtHD2a/b-silenced plants, infiltration with cryptogein led to HR-like symptoms in distal leaves. • Taken together, these results show for the first time that type-2 HDACs, which are specific to plants, act as negative regulators of elicitor-induced cell death in tobacco (Nicotiana tabacum), suggesting that the HR is controlled by post-translational modifications including (de)acetylation of nuclear proteins.
authors.affiliation: UMR INRA 1088/CNRS 5184/Université de Bourgogne Plante-Microbe-Environnement, 17 Rue Sully, BP 86510, 21065 Dijon cedex, France.|GDR CNRS N°2688 'Calcium et régulation de l'expression des gènes en contexte normal et pathologique', 31000 Toulouse, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Bourque
authors.affiliation: UMR INRA 1088/CNRS 5184/Université de Bourgogne Plante-Microbe-Environnement, 17 Rue Sully, BP 86510, 21065 Dijon cedex, France.
authors.forename: Agnès
authors.initials: A
authors.lastname: Dutartre
authors.affiliation: UMR INRA 1088/CNRS 5184/Université de Bourgogne Plante-Microbe-Environnement, 17 Rue Sully, BP 86510, 21065 Dijon cedex, France.
authors.forename: Valentin
authors.initials: V
authors.lastname: Hammoudi
authors.affiliation: UMR INRA 1088/CNRS 5184/Université de Bourgogne Plante-Microbe-Environnement, 17 Rue Sully, BP 86510, 21065 Dijon cedex, France.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Blanc
authors.affiliation: UMR INRA 1088/CNRS 5184/Université de Bourgogne Plante-Microbe-Environnement, 17 Rue Sully, BP 86510, 21065 Dijon cedex, France.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Dahan
authors.affiliation: UPSP PROXISS, AgroSup Dijon, 26 Boulevard du Dr Petitjean, BP 87999, 21079 Dijon cedex, France.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Jeandroz
authors.affiliation: Plateforme Protéomique Génopole Toulouse Midi-Pyrénées, Institut de Pharmacologie et de Biologie Structurale (IPBS), CNRS, 205 route de Narbonne, F-31077 Toulouse, France and Université Paul Sabatier, Université de Toulouse, F-31077 Toulouse, France.
authors.forename: Carole
authors.initials: C
authors.lastname: Pichereaux
authors.affiliation: Plateforme Protéomique Génopole Toulouse Midi-Pyrénées, Institut de Pharmacologie et de Biologie Structurale (IPBS), CNRS, 205 route de Narbonne, F-31077 Toulouse, France and Université Paul Sabatier, Université de Toulouse, F-31077 Toulouse, France.
authors.forename: Michel
authors.initials: M
authors.lastname: Rossignol
authors.affiliation: UMR INRA 1088/CNRS 5184/Université de Bourgogne Plante-Microbe-Environnement, 17 Rue Sully, BP 86510, 21065 Dijon cedex, France.
authors.forename: David
authors.initials: D
authors.lastname: Wendehenne
mesh_terms: D000107:Acetylation / Q000187:drug effects; D020418:Algal Proteins / Q000494:pharmacology; D000595:Amino Acid Sequence; D016923:Cell Death / Q000187:drug effects; D002853:Chromatography, Liquid; D005656:Fungal Proteins; D056464:Histone Deacetylase 2 / Q000737:chemistry / Q000378:metabolism*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism; D010455:Peptides / Q000737:chemistry; D010766:Phosphorylation / Q000187:drug effects; D010802:Phylogeny; D016415:Sequence Alignment; D014026:Nicotiana / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology*
chemical_list: D020418:Algal Proteins; D005656:Fungal Proteins; D009687:Nuclear Proteins; D010455:Peptides; C061032:cryptogein protein, Phytophthora cryptogea; D056464:Histone Deacetylase 2