title: Increased ER-mitochondrial coupling promotes mitochondrial respiration and bioenergetics during early phases of ER stress.
abstract: Increasing evidence indicates that endoplasmic reticulum (ER) stress activates the adaptive unfolded protein response (UPR), but that beyond a certain degree of ER damage, this response triggers apoptotic pathways. The general mechanisms of the UPR and its apoptotic pathways are well characterized. However, the metabolic events that occur during the adaptive phase of ER stress, before the cell death response, remain unknown. Here, we show that, during the onset of ER stress, the reticular and mitochondrial networks are redistributed towards the perinuclear area and their points of connection are increased in a microtubule-dependent fashion. A localized increase in mitochondrial transmembrane potential is observed only in redistributed mitochondria, whereas mitochondria that remain in other subcellular zones display no significant changes. Spatial re-organization of these organelles correlates with an increase in ATP levels, oxygen consumption, reductive power and increased mitochondrial Ca²⁺ uptake. Accordingly, uncoupling of the organelles or blocking Ca²⁺ transfer impaired the metabolic response, rendering cells more vulnerable to ER stress. Overall, these data indicate that ER stress induces an early increase in mitochondrial metabolism that depends crucially upon organelle coupling and Ca²⁺ transfer, which, by enhancing cellular bioenergetics, establishes the metabolic basis for the adaptation to this response.
authors.affiliation: FONDAP Center for Molecular Studies of the Cell, Faculty of Chemical and Pharmaceutical Sciences and Faculty of Medicine, University of Chile, Santiago 8380492, Chile.
authors.forename: Roberto
authors.initials: R
authors.lastname: Bravo
authors.forename: Jose Miguel
authors.initials: JM
authors.lastname: Vicencio
authors.forename: Valentina
authors.initials: V
authors.lastname: Parra
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Troncoso
authors.forename: Juan Pablo
authors.initials: JP
authors.lastname: Munoz
authors.forename: Michael
authors.initials: M
authors.lastname: Bui
authors.forename: Clara
authors.initials: C
authors.lastname: Quiroga
authors.forename: Andrea E
authors.initials: AE
authors.lastname: Rodriguez
authors.forename: Hugo E
authors.initials: HE
authors.lastname: Verdejo
authors.forename: Jorge
authors.initials: J
authors.lastname: Ferreira
authors.forename: Myriam
authors.initials: M
authors.lastname: Iglewski
authors.forename: Mario
authors.initials: M
authors.lastname: Chiong
authors.forename: Thomas
authors.initials: T
authors.lastname: Simmen
authors.forename: Antonio
authors.initials: A
authors.lastname: Zorzano
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Hill
authors.forename: Beverly A
authors.initials: BA
authors.lastname: Rothermel
authors.forename: Gyorgy
authors.initials: G
authors.lastname: Szabadkai
authors.forename: Sergio
authors.initials: S
authors.lastname: Lavandero
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D017209:Apoptosis / Q000502:physiology; D002118:Calcium / Q000378:metabolism; D019069:Cell Respiration; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D004734:Energy Metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006367:HeLa Cells; D017442:Histamine Agonists / Q000494:pharmacology; D006801:Humans; D053078:Membrane Potential, Mitochondrial; D008928:Mitochondria / Q000378:metabolism*; D010101:Oxygen Consumption / Q000187:drug effects; D018129:Phosphatidylinositol Phosphates / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology; D013312:Stress, Physiological
chemical_list: D000900:Anti-Bacterial Agents; D004791:Enzyme Inhibitors; D017442:Histamine Agonists; D018129:Phosphatidylinositol Phosphates; C060974:phosphatidylinositol 3,4,5-triphosphate; D002118:Calcium