title: Effect of boron on osteogenic differentiation of human bone marrow stromal cells.
abstract: Bone marrow stromal cells (BMSCs) have been well established as an ideal source of cell-based therapy for bone tissue engineering applications. Boron (B) is a notable trace element in humans; so far, the effects of boron on the osteogenic differentiation of BMSCs have not been reported. The aim of this study was to evaluate the effects of boron (0, 1, 10,100, and 1,000 ng/ml) on osteogenic differentiation of human BMSCs. In this study, BMSCs proliferation was analyzed by cell counting kit-8 (CCK8) assay, and cell osteogenic differentiation was evaluated by alkaline phosphatase (ALP) activity assay, Von Kossa staining, and real-time PCR. The results indicated that the proliferation of BMSCs was no different from the control group when added with B at the concentration of 1, 10, and 100 ng/ml respectively (P > 0.05); in contrast, 1,000 ng/ml B inhibited the proliferation of BMSCs at days 4, 7, and 14 (P < 0.05). By ALP staining, we discovered that BMSCs treated with 10 and 100 ng/ml B presented a higher ALP activity compared with control (P < 0.05). By real-time PCR, we detected the messenger RNA expression of ALP, osteocalcin, collagen type I, and bone morphogenetic proteins 7 were also increased in 10 and 100 ng/ml B treatment groups (P < 0.05). The calcium depositions were increased in 1 and 10 ng/ml B treatment groups (P < 0.05). Taken all together, it was the first time to report that B could increase osteogenic effect by stimulating osteogenic differentiation-related marker gene synthesis during the proliferation and differentiation phase in human BMSCs and could be a promising approach for enhancing osteogenic capacity of cell-based construction in bone tissue engineering.
authors.affiliation: Department of Orthopaedic Surgery, The Second Affiliated Hospital of Wenzhou Medical College, 109 Xue Yuan Xi Road, Wenzhou, 325000, China.
authors.forename: Xiaozhou
authors.initials: X
authors.lastname: Ying
authors.forename: Shaowen
authors.initials: S
authors.lastname: Cheng
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Zhongqin
authors.initials: Z
authors.lastname: Lin
authors.forename: Qingyu
authors.initials: Q
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Dongquan
authors.initials: D
authors.lastname: Kou
authors.forename: Yue
authors.initials: Y
authors.lastname: Shen
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Cheng
authors.forename: Ferdinand An
authors.initials: FA
authors.lastname: Rompis
authors.forename: Lei
authors.initials: L
authors.lastname: Peng
authors.forename: Chuan
authors.initials: C
authors.lastname: Zhu Lu
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D001854:Bone Marrow Cells / Q000187:drug effects*; D055419:Bone Morphogenetic Protein 7 / Q000378:metabolism; D001888:Boric Acids / Q000494:pharmacology; D001896:Boron Compounds / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D002452:Cell Count; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects; D002469:Cell Separation; D002478:Cells, Cultured; D024042:Collagen Type I / Q000096:biosynthesis; D017931:DNA Primers; D004305:Dose-Response Relationship, Drug; D005819:Genetic Markers; D006801:Humans; D015675:Osteocalcin / Q000096:biosynthesis; D010012:Osteogenesis / Q000187:drug effects* / Q000235:genetics; D010641:Phenotype; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D017154:Stromal Cells / Q000187:drug effects*
chemical_list: D055419:Bone Morphogenetic Protein 7; D001888:Boric Acids; D001896:Boron Compounds; D024042:Collagen Type I; D017931:DNA Primers; D005819:Genetic Markers; D012333:RNA, Messenger; D015675:Osteocalcin; D000469:Alkaline Phosphatase; C032688:boric acid; D002118:Calcium