title: [Induction of cell damage and change of miR-133b expression by paraquat in PC12 cells].
abstract: OBJECTIVE
  To investigate the effect of paraquat on induction of cell damage and miR-133b expression in PC12 cells.
  
  
  METHODS
  Cytotoxicity of PC12 cells was measured by MTT assay, following the PC12 cells treatment with 50, 100, or 300 µmol/L paraquat. Cell apoptosis was examined by the method of Annexin V-FITC/PI in flow cytometry (FCM) and the relative level of miR-133b expression was measured by real time RT-PCR, following the PC12 cells treatment with 100 or 300 µmol/L paraquat.
  
  
  RESULTS
  Survival rate of PC12 cells treated with 100 or 300 µmol/L paraquat was lower than that of the vehicle control group (P < 0.01, P < 0.05), in the dose dependent pattern. Apoptotic rate of PC12 cells treated with 100, 300 µmol/L paraquat was higher than that of the vehicle control group (P < 0.05). The relative level of miR-133b expression of PC12 cells treated with 300 µmol/L paraquat was higher than that of the vehicle control group (P < 0.05).
  
  
  CONCLUSIONS
  Paraquat may cause cell damage and induce apoptosis in PC12 cells, and induce miR-133b expression.
authors.affiliation: Department of Occupational and Environmental Health, Fujian Medical University, Fuzhou, China.
authors.forename: Huang-yuan
authors.initials: HY
authors.lastname: Li
authors.forename: Si-ying
authors.initials: SY
authors.lastname: Wu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D035683:MicroRNAs / Q000378:metabolism*; D016716:PC12 Cells; D010269:Paraquat / Q000633:toxicity*; D051381:Rats
chemical_list: C549446:MIRN133 microRNA, rat; D035683:MicroRNAs; D010269:Paraquat