title: Generation of induced pluripotent stem cells from urine.
abstract: Forced expression of selected transcription factors can transform somatic cells into embryonic stem cell (ESC)-like cells, termed induced pluripotent stem cells (iPSCs). There is no consensus regarding the preferred tissue from which to harvest donor cells for reprogramming into iPSCs, and some donor cell types may be more prone than others to accumulation of epigenetic imprints and somatic cell mutations. Here, we present a simple, reproducible, noninvasive method for generating human iPSCs from renal tubular cells present in urine. This procedure eliminates many problems associated with other protocols, and the resulting iPSCs display an excellent ability to differentiate. These data suggest that urine may be a preferred source for generating iPSCs.
authors.affiliation: Stem Cell and Cancer Biology Group, Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Chinese Academy of Sciences, Guangzhou, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Zhou
authors.forename: Christina
authors.initials: C
authors.lastname: Benda
authors.forename: Sarah
authors.initials: S
authors.lastname: Duzinger
authors.forename: Yinghua
authors.initials: Y
authors.lastname: Huang
authors.forename: Xingyan
authors.initials: X
authors.lastname: Li
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Li
authors.forename: Xiangpeng
authors.initials: X
authors.lastname: Guo
authors.forename: Guokun
authors.initials: G
authors.lastname: Cao
authors.forename: Shen
authors.initials: S
authors.lastname: Chen
authors.forename: Lili
authors.initials: L
authors.lastname: Hao
authors.forename: Yau-Chi
authors.initials: YC
authors.lastname: Chan
authors.forename: Kwong-Man
authors.initials: KM
authors.lastname: Ng
authors.forename: Jenny Cy
authors.initials: JC
authors.lastname: Ho
authors.forename: Matthias
authors.initials: M
authors.lastname: Wieser
authors.forename: Jiayan
authors.initials: J
authors.lastname: Wu
authors.forename: Heinz
authors.initials: H
authors.lastname: Redl
authors.forename: Hung-Fat
authors.initials: HF
authors.lastname: Tse
authors.forename: Johannes
authors.initials: J
authors.lastname: Grillari
authors.forename: Regina
authors.initials: R
authors.lastname: Grillari-Voglauer
authors.forename: Duanqing
authors.initials: D
authors.lastname: Pei
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Esteban
mesh_terms: D000368:Aged; D005260:Female; D018014:Gene Transfer Techniques; D006801:Humans; D057026:Induced Pluripotent Stem Cells; D007684:Kidney Tubules / Q000166:cytology*; D008297:Male; D014556:Urine / Q000166:cytology*; D055815:Young Adult