title: Generation of the tamoxifen-inducible DBH-Cre transgenic mouse line DBH-CT.
abstract: We generated transgenic mice bearing a tamoxifen-dependent Cre recombinase expressed under the control of the dopamine-β-hydroxylase promoter. By crossing to the ROSA26 reporter mice we show that tamoxifen-induced Cre recombinase in adult mice specifically activates β-galactosidase expression in differentiated noradrenergic neurons of the central and peripheral nervous system. Tamoxifen application in adult mice did not induce β-galactosidase activity in parasympathetic neurons that transiently express DBH during development. Thus, this transgenic mouse line represents a valuable tool to study gene function in mature noradrenergic neurons by conditional inactivation.
authors.affiliation: Department of Neurochemistry, Research Group Developmental Neurobiology, Max-Planck-Institute for Brain Research, Frankfurt/M, Germany.
authors.forename: Jutta
authors.initials: J
authors.lastname: Stubbusch
authors.forename: Afsaneh
authors.initials: A
authors.lastname: Majdazari
authors.forename: Mirko
authors.initials: M
authors.lastname: Schmidt
authors.forename: Günther
authors.initials: G
authors.lastname: Schütz
authors.forename: Thomas
authors.initials: T
authors.lastname: Deller
authors.forename: Hermann
authors.initials: H
authors.lastname: Rohrer
mesh_terms: D059331:Adrenergic Neurons / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D003433:Crosses, Genetic; D004299:Dopamine beta-Hydroxylase / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D005838:Genotype; D017403:In Situ Hybridization / Q000379:methods; D019426:Integrases / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D023421:Models, Animal; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D022661:RNA, Untranslated; D011995:Recombination, Genetic; D013629:Tamoxifen / Q000494:pharmacology*; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
chemical_list: C110853:Gt(ROSA)26Sor non-coding RNA, mouse; D011506:Proteins; D022661:RNA, Untranslated; D013629:Tamoxifen; D004299:Dopamine beta-Hydroxylase; C045073:Cre recombinase; D019426:Integrases; D001616:beta-Galactosidase