title: No mutation but high mRNA expression of Coxsackie-Adenovirus Receptor was observed in both dilated and ischemic cardiomyopathy.
abstract: The most common causes of acute myocarditis and the possible consequence of dilated cardiomyopathy are virus infections. The receptor of the two most common viruses connected to these myocardial diseases was identified as Coxsackie-Adenovirus Receptor. The purpose of this study was to assess Coxsackie-Adenovirus Receptor mRNA expression in the myocardium and search for mutations in the Coxsackie-Adenovirus Receptor gene to compare dilated, inflammatory and ischemic cardiomyopathy with control group. All the myocardial samples were obtained from 35 explanted hearts during heart transplantation, than DNA and RNA were isolated from the muscle samples. cDNA was generated from RNA using reverse transcription, and real-time PCR was performed with relative quantification by β-actin gene as endogenous control. Using DNA extracted from the myocardial samples, we sequenced all the seven exons of the Coxsackie-Adenovirus Receptor gene. Coxsackie-Adenovirus Receptor mRNA expression was higher in both ischemic and dilated cardiomyopathy groups than in inflammatory cardiomyopathy and healthy control groups. Sequencing of CAR gene showed no sign of mutation. Therefore, the sequences result of CAR exons did not show any mutation or polymorphism, that explains a determinant role of CAR in dilated or ischemic CM. Our results suggest that high mRNA expression of Coxsackie-Adenovirus Receptor may support its role in regeneration of the damaged myocardium rather than having any role in viral mediated heart disease.
authors.affiliation: Department of Forensic and Insurance Medicine, Semmelweis University, 93 Ulloi Str, Budapest H-1091, Hungary. tatraie@freemail.hu
authors.forename: Eniko
authors.initials: E
authors.lastname: Tatrai
authors.forename: Katalin
authors.initials: K
authors.lastname: Bedi
authors.forename: Ilona
authors.initials: I
authors.lastname: Kovalszky
authors.forename: Istvan
authors.initials: I
authors.lastname: Hartyanszky
authors.forename: Andras
authors.initials: A
authors.lastname: Laszik
authors.forename: Gyorgy
authors.initials: G
authors.lastname: Acsady
authors.forename: Peter
authors.initials: P
authors.lastname: Sotonyi
authors.forename: Marta
authors.initials: M
authors.lastname: Hubay
mesh_terms: D000293:Adolescent; D000328:Adult; D002311:Cardiomyopathy, Dilated / Q000473:pathology / Q000821:virology*; D016022:Case-Control Studies; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D003384:Coxsackievirus Infections / Q000175:diagnosis / Q000473:pathology*; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D005554:Forensic Medicine / Q000379:methods; D015967:Gene Expression Regulation, Viral; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D017202:Myocardial Ischemia / Q000473:pathology / Q000821:virology*; D009205:Myocarditis / Q000473:pathology / Q000821:virology; D009206:Myocardium / Q000473:pathology; D012333:RNA, Messenger / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction; D011991:Receptors, Virus / Q000235:genetics / Q000378:metabolism*; D012016:Reference Values; D015854:Up-Regulation; D055815:Young Adult
chemical_list: C568771:CLMP protein, human; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D004279:DNA, Viral; D012333:RNA, Messenger; D011991:Receptors, Virus