title: The quantitative detection of aripiprazole and its main metabolite by using capillary-electrophoresis.
abstract: BACKGROUND
  We aimed to establish a feasible and reliable method to measure the level of aripiprazole and its main metabolite, dehydroaripiprazole, using a capillary-electrophoresis (CE) machine.
  
  
  METHODS
  Two blood samples were obtained from psychiatric patients hospitalized in Yu-Li Hospital who had been treated with aripiprazole for more than 4 weeks, at least 10 mg/d. Conditions for voltage, temperature and buffer concentration was optimized on a CE machine.
  
  
  RESULTS
  The most optimal conditions for CE were 80 mM 2-3% DMSO-phosphate as a buffer under pH = 3.0, 15 KV, 20°C and a detection wavelength of 214 nm. The linear ranges of aripiprazole and dehydroaripiprazole concentration were from 0.5 to 50 ng/mL.
  
  
  CONCLUSIONS
  CE method is a feasible method to measure aripiprazole level with relatively low price compared with other analytical techniques for clinical use.
authors.affiliation: Department of Psychiatry, Taichung Veterans General Hospital, Taichung, Taiwan, ROC.
authors.forename: Chia-Jui
authors.initials: CJ
authors.lastname: Tsai
authors.forename: Yen-Hsin
authors.initials: YH
authors.lastname: Yu
authors.forename: Hsien-Jane
authors.initials: HJ
authors.lastname: Chiu
authors.forename: El-Wui
authors.initials: el-W
authors.lastname: Loh
authors.forename: Jau-Tay
authors.initials: JT
authors.lastname: Wang
authors.forename: Chin-Hong
authors.initials: CH
authors.lastname: Chan
authors.forename: Tsuo-Hung
authors.initials: TH
authors.lastname: Lan
mesh_terms: D014150:Antipsychotic Agents / Q000097:blood*; D000068180:Aripiprazole; D002138:Calibration; D019075:Electrophoresis, Capillary / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D010879:Piperazines / Q000097:blood* / Q000378:metabolism; D015363:Quinolones / Q000097:blood* / Q000378:metabolism
chemical_list: D014150:Antipsychotic Agents; D010879:Piperazines; D015363:Quinolones; D000068180:Aripiprazole