title: Fucoidin, a neutrophil rolling inhibitor, reduces damage in a rat electrical burn injury model.
abstract: BACKGROUND
  Electrical injuries induce progressive tissue loss caused by free oxygen radicals released from neutrophil aggregates. Fucoidin, a potent inhibitor of L-selectin function, reduces the aggregation of neutrophils. The aim of this study was to evaluate the effect of fucoidin on tissue damage in rat electrical burn injury model.
  
  
  METHODS
  Forty-two male Wistar albino rats (250-300 g) were divided into 3 groups (Group A (n=6), control group without electrical burn injury; Groups B (n=18) and C (n=18), electrical burn injury groups without and with fucoidin therapy, respectively). Three separate analyses were performed at different time points on 6 out of 18 mice from Group B and C at each time point. Biochemistry (myeloperoxidase and malondialdehyde levels) and histopathology (number of neutrophils) of the skin and muscle biopsies at 1st hour; tissue edema (ratio of wet weight/dry weight of extremities) at 24th hour; and necrotic areas at 7th day after electrical injury were evaluated. The electrical burn was induced by exposing rats to 220 V AC between their left upper extremity and right lower extremity for 10 s. Fucoidin was administered as 25 mg/kg intravenous bolus injection at 15 min after electrical burn injury.
  
  
  RESULTS
  Myeloperoxidase and malondialdehyde levels, number of neutrophils, tissue edema, and necrotic area were significantly less in fucoidin-applied rats than the group without fucoidin therapy.
  
  
  CONCLUSIONS
  Fucoidin inhibits tissue damage induced by electrical burn injury in rats by reducing necrotic area, edema and number of neutrophils.
authors.affiliation: Department of Plastic Reconstructive and Aesthetic Surgery, Trakya University Faculty of Medicine, 22030 Edirne, Turkey. drebenlier@hotmail.com
authors.forename: Erol
authors.initials: E
authors.lastname: Benlier
authors.forename: Sevgi
authors.initials: S
authors.lastname: Eskiocak
authors.forename: Fulya Oz
authors.initials: FO
authors.lastname: Puyan
authors.forename: Huseyin
authors.initials: H
authors.lastname: Kandulu
authors.forename: Yasin
authors.initials: Y
authors.lastname: Unal
authors.forename: Husamettin
authors.initials: H
authors.lastname: Top
authors.forename: Ahmet Cemal
authors.initials: AC
authors.lastname: Aygit
mesh_terms: D000818:Animals; D000925:Anticoagulants / Q000008:administration & dosage / Q000627:therapeutic use*; D002058:Burns, Electric / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D004487:Edema / Q000473:pathology; D007275:Injections, Intravenous; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D023421:Models, Animal; D018482:Muscle, Skeletal / Q000378:metabolism / Q000473:pathology; D009336:Necrosis / Q000473:pathology; D009504:Neutrophils / Q000166:cytology; D009195:Peroxidase / Q000378:metabolism; D011134:Polysaccharides / Q000008:administration & dosage / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000378:metabolism / Q000473:pathology
chemical_list: D000925:Anticoagulants; D011134:Polysaccharides; D008315:Malondialdehyde; C007789:fucoidan; D009195:Peroxidase