title: Transferability and characterization of microsatellite markers in two Neotropical Ficus species.
abstract: Microsatellite markers were transferred and characterized for two Neotropical fig tree species, Ficus citrifolia and Ficus eximia. Our study demonstrated that microsatellite markers developed from different subgenera of Ficus can be transferred to related species. In the present case, 12 of the 15 primer pairs tested (80%) were successfully transferred to both of the above species. Eleven loci were polymorphic when tested across 60 F. citrifolia and 60 F. eximia individuals. For F. citrifolia, there were 4 to 15 alleles per locus, whereas expected heterozygosities ranged from 0.31 to 0.91. In the case of F. eximia, this was 2 to 12 alleles per locus and expected heterozygosities from 0.42 to 0.87.
authors.affiliation: Programa de Pós-graduação em Biologia Comparada, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP Brazil.
authors.forename: Alison Gonçalves
authors.initials: AG
authors.lastname: Nazareno
authors.forename: Rodrigo Augusto Santinelo
authors.initials: RA
authors.lastname: Pereira
authors.forename: Juliana Massimino
authors.initials: JM
authors.lastname: Feres
authors.forename: Moacyr Antônio
authors.initials: MA
authors.lastname: Mestriner
authors.forename: Ana Lilia
authors.initials: AL
authors.lastname: Alzate-Marin