title: How modeling can reconcile apparently discrepant experimental results: the case of pacemaking in dopaminergic neurons.
abstract: Midbrain dopaminergic neurons are endowed with endogenous slow pacemaking properties. In recent years, many different groups have studied the basis for this phenomenon, often with conflicting conclusions. In particular, the role of a slowly-inactivating L-type calcium channel in the depolarizing phase between spikes is controversial, and the analysis of slow oscillatory potential (SOP) recordings during the blockade of sodium channels has led to conflicting conclusions. Based on a minimal model of a dopaminergic neuron, our analysis suggests that the same experimental protocol may lead to drastically different observations in almost identical neurons. For example, complete L-type calcium channel blockade eliminates spontaneous firing or has almost no effect in two neurons differing by less than 1% in their maximal sodium conductance. The same prediction can be reproduced in a state of the art detailed model of a dopaminergic neuron. Some of these predictions are confirmed experimentally using single-cell recordings in brain slices. Our minimal model exhibits SOPs when sodium channels are blocked, these SOPs being uncorrelated with the spiking activity, as has been shown experimentally. We also show that block of a specific conductance (in this case, the SK conductance) can have a different effect on these two oscillatory behaviors (pacemaking and SOPs), despite the fact that they have the same initiating mechanism. These results highlight the fact that computational approaches, besides their well known confirmatory and predictive interests in neurophysiology, may also be useful to resolve apparent discrepancies between experimental results.
authors.affiliation: Laboratory of Pharmacology and GIGA Neurosciences, University of Liège, Liège, Belgium.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Drion
authors.forename: Laurent
authors.initials: L
authors.lastname: Massotte
authors.forename: Rodolphe
authors.initials: R
authors.lastname: Sepulchre
authors.forename: Vincent
authors.initials: V
authors.lastname: Seutin
mesh_terms: D000200:Action Potentials / Q000187:drug effects / Q000502:physiology; D000704:Analysis of Variance; D000818:Animals; D015220:Calcium Channels / Q000502:physiology; D019295:Computational Biology; D004298:Dopamine / Q000502:physiology*; D015259:Dopamine Agents / Q000494:pharmacology; D008297:Male; D008636:Mesencephalon / Q000166:cytology; D008954:Models, Biological; D009474:Neurons / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D015222:Sodium Channels / Q000502:physiology
chemical_list: D015220:Calcium Channels; D015259:Dopamine Agents; D015222:Sodium Channels; D004298:Dopamine