title: Development of an extended multilocus sequence typing for genotyping of Brucella isolates.
abstract: By amplifying and sequencing longer sequences, an extended multi locus sequence typing (EMLST) theme was developed for Brucella. 61 isolates were genotyped by the EMLST with increased resolution. This strategy could be extended to other bacteria to improve MLST genotyping resolution without additional loci.
authors.affiliation: School of Public Health, Key Laboratory of Zoonosis, Ministry of Education, Jilin University, Changchun, Jilin 130021, PR China.
authors.forename: Yanfen
authors.initials: Y
authors.lastname: Chen
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhen
authors.forename: Yufei
authors.initials: Y
authors.lastname: Wang
authors.forename: Jie
authors.initials: J
authors.lastname: Xu
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Sun
authors.forename: Tiefeng
authors.initials: T
authors.lastname: Li
authors.forename: Lina
authors.initials: L
authors.lastname: Gao
authors.forename: Fei
authors.initials: F
authors.lastname: Guo
authors.forename: Dali
authors.initials: D
authors.lastname: Wang
authors.forename: Xitong
authors.initials: X
authors.lastname: Yuan
authors.forename: Jing
authors.initials: J
authors.lastname: Yuan
authors.forename: Liuyu
authors.initials: L
authors.lastname: Huang
authors.forename: Zeliang
authors.initials: Z
authors.lastname: Chen
authors.forename: Yaqin
authors.initials: Y
authors.lastname: Yu
mesh_terms: D002002:Brucella / Q000145:classification* / Q000235:genetics*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D058885:Multilocus Sequence Typing / Q000379:methods*
chemical_list: D004269:DNA, Bacterial