title: Bilayer lipid membrane biosensor with enhanced stability for amperometric determination of hydrogen peroxide.
abstract: In this paper, a polydopamine (PDA) film is electropolymerized on the surface of bilayer lipid membrane (BLM) which is immobilized with horseradish peroxidase (HRP). The coverage of the PDA film on HRP/BLM electrode is monitored by electrochemical impedance spectroscopy (EIS). The electrocatalytic reduction of H(2)O(2) at the PDA/HRP/BLM electrode is studied by means of cyclic voltammetry (CV). The biosensor has a fast response to H(2)O(2) of less than 5s and an excellent linear relationship is obtained in the concentration range from 2.5×10(-7) to 3.1×10(-3) molL(-1), with a detection limit of 1.0×10(-7) molL(-1) (S/N=3). The response current of BLM/HRP/PDA biosensor retains 84% of its original response after being stored in 0.1 molL(-1) pH 7.0 PBS at 4°C for 3 weeks. The selectivity, repeatability, and storage stability of PDA/HRP/BLM biosensor are greatly enhanced by the coverage of polydopamine film on BLM.
authors.affiliation: Department of Chemistry and Chemical Engineering, East China Jiao Tong University, Nanchang, Jiangxi 330013, PR China. zlz@ecjtu.jx.cn
authors.forename: Longzhen
authors.initials: L
authors.lastname: Zheng
authors.forename: Leyan
authors.initials: L
authors.lastname: Xiong
authors.forename: Dan
authors.initials: D
authors.lastname: Zheng
authors.forename: Yindi
authors.initials: Y
authors.lastname: Li
authors.forename: Qiang
authors.initials: Q
authors.lastname: Liu
authors.forename: Kui
authors.initials: K
authors.lastname: Han
authors.forename: Wen
authors.initials: W
authors.lastname: Liu
authors.forename: Kun
authors.initials: K
authors.lastname: Tao
authors.forename: Shaoming
authors.initials: S
authors.lastname: Yang
authors.forename: Jian
authors.initials: J
authors.lastname: Xia
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004298:Dopamine / Q000031:analogs & derivatives; D055664:Electrochemical Techniques; D004566:Electrodes; D006861:Hydrogen Peroxide / Q000032:analysis*; D057230:Limit of Detection; D008051:Lipid Bilayers / Q000737:chemistry*; D058105:Polymerization
chemical_list: D008051:Lipid Bilayers; D006861:Hydrogen Peroxide; D004298:Dopamine