title: [Human chorionic gonadotrophin on the expression of epidermal growth factor in rat renal tissues].
abstract: OBJECTIVE
  To investigate hCG on the expression of epidermal growth factor (EGF) in renal tissues.
  
  
  METHODS
  20 male SD rats weighed 300 - 350 g were injected hCG 200 IU/d for 7 days, and 20 male rats were injected sa-line at equal volume as a control. Kidney tissues were per-formed with immunohistochemical staining to evaluate the distribution of EGF and quantitative PCR (Q-PCR) was used to evaluate the EGF mRNA.
  
  
  RESULTS
  Immunohistochemtry showed that the positive rate of EGF in experimental group was significantly higher than that in control group(P<0.01), but Q-PCR results showed no significant difference between two groups ( P > 0. 05).
  
  
  CONCLUSIONS
  hCG increased the concentration of EGF by maturing pre-pro-EGF not expressing EGF from de novo synthesis.
authors.affiliation: Department of Nephrology, 1st Affiliated Hospital of Guangzhou Medical College, Guangzhou 510120, China. lucasays@gmail.com
authors.forename: Lu-jia
authors.initials: LJ
authors.lastname: Zhang
authors.forename: Xiu-meng
authors.initials: XM
authors.lastname: Chen
authors.forename: Rujian
authors.initials: R
authors.lastname: Cui
authors.forename: Jing-yan
authors.initials: JY
authors.lastname: Lin
authors.forename: Xian-yang
authors.initials: XY
authors.lastname: OU
authors.forename: Hua-lin
authors.initials: HL
authors.lastname: Wang
mesh_terms: D000818:Animals; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D004815:Epidermal Growth Factor / Q000187:drug effects / Q000378:metabolism*; D007150:Immunohistochemistry; D007668:Kidney / Q000166:cytology / Q000378:metabolism*; D008297:Male; D011498:Protein Precursors / Q000187:drug effects*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D006063:Chorionic Gonadotropin; D011498:Protein Precursors; D012333:RNA, Messenger; C042652:epidermal growth factor precursor; D004815:Epidermal Growth Factor