title: Expression analysis and response of Penaeus monodon 14-3-3 genes to salinity stress.
abstract: Two isoforms of 14-3-3 protein, namely 14-3-3A and 14-3-3B, from the shrimp Penaeus monodon were investigated for their potential role in adaptation to salinity stress. Transcripts of 14-3-3A were found in various shrimp tissues whilst expression of 14-3-3B transcripts was more specific being observed in the osmoregulatory tissues, that is in the gills and epipodites. In shrimp gills, the 14-3-3A transcript levels slightly changed after transfer of the shrimp from 3 to 25 or to 40 ppt. In contrast, significant change in the mRNA levels in response to salinity stress was detected for 14-3-3B, where a significant decrease of 14-3-3B transcript was observed in gills of shrimp transferred from hypo-osmotic (3 ppt) salinity to iso-osmotic or hyper-osmotic (25 and 40ppt, respectively) salinity. On the other hand, shrimp transferred from 40 ppt to 3 ppt showed a strong induction of 14-3-3B mRNA expression in the gills. These transcript expression analyses suggest that 14-3-3B is likely to be involved in the hyper-osmotic regulation in P. monodon. In addition, 14-3-3B appeared to regulate ATPase function since suppression of the gene by RNA interference resulted in a significant decrease in the total ATPase activity.
authors.affiliation: Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Chulalongkorn University, Bangkok, Thailand.
authors.forename: Montira
authors.initials: M
authors.lastname: Kaeodee
authors.forename: Siriporn
authors.initials: S
authors.lastname: Pongsomboon
authors.forename: Anchalee
authors.initials: A
authors.lastname: Tassanakajon
mesh_terms: D048948:14-3-3 Proteins / Q000737:chemistry / Q000172:deficiency / Q000235:genetics* / Q000378:metabolism; D000222:Adaptation, Physiological; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D030541:Databases, Genetic; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D009997:Osmotic Pressure; D033561:Penaeidae / Q000235:genetics* / Q000502:physiology*; D020033:Protein Isoforms / Q000737:chemistry / Q000172:deficiency / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D054712:Salinity; D013312:Stress, Physiological / Q000235:genetics*; D014882:Water-Electrolyte Balance / Q000235:genetics
chemical_list: D048948:14-3-3 Proteins; D020033:Protein Isoforms; D012333:RNA, Messenger; D000251:Adenosine Triphosphatases