title: nPEG-TiO₂ nanoparticles: a facile route to elaborate nanostructured surfaces for biological applications.
abstract: We report the synthesis of diacid-terminated PEG-functionalized cubic TiO(2) nanocrystals by a simple one-step solvothermal method, and their further use to form nanostructured surfaces for protein immobilization. The relevance and major interest of the so-obtained nanocrystals are the presence of terminal carboxylic acid groups at their surface, as confirmed by infrared analyses, in addition to the surrounding PEG chains, essential to avoid non specific interactions. These functional chemical groups were used to (i) immobilize the synthesized nanocubes on a cysteamine-modified Au surface, and to (ii) attach proteins via a presumable covalent link. AFM images show that the shapes and the narrow size distribution of the nanocubes, observed by TEM, were preserved after their immobilization on the modified Au surface. Moreover, the efficiency and specificity of antigen recognition were demonstrated using spectroscopic analyses. Our successful approach provides a versatile and facile way to elaborate specific and sensitive nanostructured surfaces for biosensors.
authors.affiliation: Laboratoire de Réactivité de Surface, Université Pierre et Marie Curie -Paris 06, CNRS UMR 7197, 4 Place Jussieu, Case 178, F-75005 Paris, France.
authors.forename: J
authors.initials: J
authors.lastname: Spadavecchia
authors.forename: S
authors.initials: S
authors.lastname: Boujday
authors.forename: J
authors.initials: J
authors.lastname: Landoulsi
authors.forename: C-M
authors.initials: CM
authors.lastname: Pradier
mesh_terms: D018625:Microscopy, Atomic Force; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles; D011092:Polyethylene Glycols / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: C009495:titanium dioxide; D011092:Polyethylene Glycols; D014025:Titanium