title: Quantitative peptidomic analysis by a newly developed one-step direct transfer technology without depletion of major blood proteins: its potential utility for monitoring of pathophysiological status in pregnancy-induced hypertension.
abstract: We have recently developed a new target plate (BLOTCHIP®) for MALDI-MS. An advantage of this procedure is that it does not require the lowering of protein concentrations in test samples prior to analysis. Accordingly, this new technology enables the detection of peptides present in blood samples, including those that would otherwise be adsorbed to abundant blood proteins and would thus escape detection. Using this technology, we analyzed the peripheral blood of patients with pregnancy-induced hypertension (PIH; the most common serious complication of pregnancy) to test a potential utility of the technology for monitoring of the pathophysiological status. In the present study, we found 23 characteristic peptides for PIH in the blood serum of pregnant women. Offline LC-MALDI MS/MS identified 7 of the 23 peptides as fragments derived from kininogen-1 (three peptides), fibrinogen-α, complement component C4-A/B, α-2-HS-glycoprotein and inter-α-trypsin inhibitor heavy chain H4. 2-D scatter plots with combinations of the peptides found in the present study can be grouped for pregnant women with/without PIH, which would be satisfactory reflected for their status. Additionally, the levels of most of these peptides found were significantly decreased by albumin/IgG depletion prior to BLOTCHIP® analysis in accordance with conventional proteomics procedures. These results indicated that BLOTCHIP® analysis can be applied for discovery study of PIH biomarker candidates.
authors.affiliation: Institute for Environmental and Gender-Specific Medicine, Juntendo University Graduate School of Medicine, Urayasu, Japan. yaraki@juntendo.ac.jp
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Araki
authors.forename: Daisuke
authors.initials: D
authors.lastname: Nonaka
authors.forename: Atsushi
authors.initials: A
authors.lastname: Tajima
authors.forename: Mayuko
authors.initials: M
authors.lastname: Maruyama
authors.forename: Takeaki
authors.initials: T
authors.lastname: Nitto
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Ishikawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yoshitake
authors.forename: Emiko
authors.initials: E
authors.lastname: Yoshida
authors.forename: Noriko
authors.initials: N
authors.lastname: Kuronaka
authors.forename: Kyoichi
authors.initials: K
authors.lastname: Asada
authors.forename: Mitsuaki
authors.initials: M
authors.lastname: Yanagida
authors.forename: Michio
authors.initials: M
authors.lastname: Nojima
authors.forename: Koyo
authors.initials: K
authors.lastname: Yoshida
authors.forename: Kenji
authors.initials: K
authors.lastname: Takamori
authors.forename: Teruto
authors.initials: T
authors.lastname: Hashiguchi
authors.forename: Ikuro
authors.initials: I
authors.lastname: Maruyama
authors.forename: Lyang-Ja
authors.initials: LJ
authors.lastname: Lee
authors.forename: Kenji
authors.initials: K
authors.lastname: Tanaka
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood*; D001798:Blood Proteins / Q000032:analysis*; D001920:Bradykinin / Q000097:blood; D005260:Female; D006801:Humans; D046110:Hypertension, Pregnancy-Induced / Q000097:blood* / Q000503:physiopathology*; D010455:Peptides / Q000032:analysis* / Q000235:genetics; D011247:Pregnancy; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D015415:Biomarkers; D001798:Blood Proteins; D010455:Peptides; D020543:Proteome; D001920:Bradykinin