title: Volunteer challenge with enterotoxigenic Escherichia coli that express intestinal colonization factor fimbriae CS17 and CS19.
abstract: Human challenges with enterotoxigenic Escherichia coli (ETEC) have broadened our understanding of this important enteropathogen. We report findings from the first challenge studies using ETEC-expressing colonization factor fimbria CS17 and CS19. LSN03-016011/A (LT, CS17) elicited a dose-dependent effect, with the upper dose (6 × 10(9) organisms) causing diarrhea in 88% of recipients. WS0115A (LTSTp, CS19) also showed a dose response, with a 44% diarrhea rate at 9 × 10(9) organisms. Both strains elicited homologous antifimbrial and anti-LT antibody seroconversion. These studies establish the relative pathogenicity of ETEC expressing newer class 5 fimbriae and suggest suitability of the LT|CS17-ETEC challenge model for interventional trials.
authors.affiliation: Department of Medicine, Johns Hopkins University, Johns Hopkins Bayview Medical Center, MD, USA.
authors.forename: Robin
authors.initials: R
authors.lastname: McKenzie
authors.forename: Chad K
authors.initials: CK
authors.lastname: Porter
authors.forename: Joyce A
authors.initials: JA
authors.lastname: Cantrell
authors.forename: Barbara
authors.initials: B
authors.lastname: Denearing
authors.forename: Aisling
authors.initials: A
authors.lastname: O'Dowd
authors.forename: Shannon L
authors.initials: SL
authors.lastname: Grahek
authors.forename: Stephanie A
authors.initials: SA
authors.lastname: Sincock
authors.forename: Colleen
authors.initials: C
authors.lastname: Woods
authors.forename: Peter
authors.initials: P
authors.lastname: Sebeny
authors.forename: David A
authors.initials: DA
authors.lastname: Sack
authors.forename: David R
authors.initials: DR
authors.lastname: Tribble
authors.forename: A Louis
authors.initials: AL
authors.lastname: Bourgeois
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Savarino
mesh_terms: D018829:Adhesins, Bacterial / Q000096:biosynthesis* / Q000276:immunology; D000293:Adolescent; D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood; D001427:Bacterial Toxins / Q000276:immunology; D003967:Diarrhea / Q000382:microbiology / Q000473:pathology; D054307:Enterotoxigenic Escherichia coli / Q000254:growth & development / Q000472:pathogenicity*; D004768:Enterotoxins / Q000276:immunology; D004927:Escherichia coli Infections / Q000382:microbiology* / Q000473:pathology*; D029968:Escherichia coli Proteins / Q000096:biosynthesis* / Q000276:immunology; D005260:Female; D006805:Human Experimentation; D006801:Humans; D008297:Male; D008875:Middle Aged; D037521:Virulence Factors / Q000096:biosynthesis* / Q000276:immunology; D055815:Young Adult
chemical_list: D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial; D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D037521:Virulence Factors; C027538:heat-labile enterotoxin, E coli