title: [Association between single nucleotide polymorphisms of 5'-untranslated region of GPx4 gene and male infertility].
abstract: OBJECTIVE
  To study the association between the single nucleotide polymorphisms (SNPs) of the 5'-untranslated region (5'-UTR) of phospholipid hydroperoxide glutathione peroxidase (GPx4 or PHGPx) gene and oligo- or asthenozoospermic male infertility.
  
  
  METHODS
  The 5'-UTR region of the GPx4 gene was amplified from infertile men and controls using the polymerase chain reaction and was analyzed for polymorphisms by direct sequencing.
  
  
  RESULTS
  A total of 9 SNPs were present in the cohort, however there were no significant differences in these 9 SNPs between the case and control groups. According to the results of linkage disequilibrium analysis and haplotype construction, one haplotype (rs757229-rs757230-rs4588110-rs3746165-rs3746166: C-G-G-T-A) was present only in the control men, and significant difference was detected(P< 0.01).
  
  
  CONCLUSIONS
  The SNPs of 5'-UTR region of the GPx4 gene might not be associated with oligo- or asthenozoospermic male infertility. However, the haplotype (rs757229-rs757230-rs4588110- rs3746165-rs3746166: C-G-G-T-A) might be a protective haplotype.
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, Yunnan, 650118 P. R. China.
authors.forename: Shu-yuan
authors.initials: SY
authors.lastname: Liu
authors.forename: Chang-jun
authors.initials: CJ
authors.lastname: Zhang
authors.forename: Xiao-min
authors.initials: XM
authors.lastname: Si
authors.forename: Yu-feng
authors.initials: YF
authors.lastname: Yao
authors.forename: Lei
authors.initials: L
authors.lastname: Shi
authors.forename: Jin-kun
authors.initials: JK
authors.lastname: Ke
authors.forename: Liang
authors.initials: L
authors.lastname: Yu
authors.forename: Li
authors.initials: L
authors.lastname: Shi
authors.forename: Zhao-qin
authors.initials: ZQ
authors.lastname: Yang
authors.forename: Xiao-qin
authors.initials: XQ
authors.lastname: Huang
authors.forename: Hao
authors.initials: H
authors.lastname: Sun
authors.forename: Jia-you
authors.initials: JY
authors.lastname: Chu
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics*; D000328:Adult; D000483:Alleles; D005787:Gene Frequency; D005838:Genotype; D005979:Glutathione Peroxidase / Q000235:genetics*; D006801:Humans; D007248:Infertility, Male / Q000235:genetics*; D015810:Linkage Disequilibrium / Q000235:genetics; D008297:Male; D008875:Middle Aged; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D055815:Young Adult
chemical_list: D020121:5' Untranslated Regions; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D005979:Glutathione Peroxidase