title: Novel IFNγ ELISPOT assay for detection of functional carcinoembryonic antigen-specific chimeric antigen receptor-redirected T cells.
abstract: We here describe the development of a novel ELISPOT assay for the detection and enumeration of IFNγ-secreting functional chimeric antigen receptor (CAR)-redirected T cells against carcinoembryonic antigen (CEA). This method is valuable for clinical trials to monitor the presence of functional CEA-specific T cells transduced with a CAR. The same principle should be applicable for the detection of functional CAR-redirected T cells against any other tumour-associated antigens by immobilizing a particular biotinylated antigen to streptavidin-coated beads.
authors.affiliation: Centre for Biochemistry, Drug Design and Cancer Research, School of Environment & Life Sciences, University of Salford, Salford, UK. e.elkord@salford.ac.uk
authors.forename: E
authors.initials: E
authors.lastname: Elkord
authors.forename: D J
authors.initials: DJ
authors.lastname: Burt
mesh_terms: D019921:Biotinylation; D002272:Carcinoembryonic Antigen / Q000276:immunology* / Q000302:isolation & purification; D058501:Enzyme-Linked Immunospot Assay / Q000379:methods*; D006801:Humans; D007371:Interferon-gamma / Q000032:analysis*; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D011993:Recombinant Fusion Proteins; D019809:Streptavidin; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D002272:Carcinoembryonic Antigen; D011948:Receptors, Antigen, T-Cell; D011993:Recombinant Fusion Proteins; D007371:Interferon-gamma; D019809:Streptavidin