title: Susceptibility to re-infection in C57BL/6 mice with recombinant strains of Toxoplasma gondii.
abstract: This work reports results of re-infection of BALB/c and C57BL/6 mice with different recombinant strains of Toxoplasma gondii. Mice were prime-infected with the non-virulent D8 strain and challenged with virulent strains. PCR-RFLP of cS10-A6 genetic marker of T. gondii demonstrated that BALB/c mice were re-infected with the EGS strain, while C57BL/6 mice were re-infected with the EGS and CH3 strains. Levels of IFN-γ and IL-10 after D8 prime-infection were lower in C57BL/6 than in BALB/c mice. Brain inflammation after D8 prime-infection was more intense in C57BL/6 than in BALB/c mice. It was shown that re-infection depends on mice lineage and genotype of the strain used in the challenge.
authors.affiliation: Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais-UFMG, Av. Antonio Carlos 6627, 31270-901 Belo Horizonte, MG, Brazil.
authors.forename: Geane P
authors.initials: GP
authors.lastname: Brandão
authors.forename: Maria N
authors.initials: MN
authors.lastname: Melo
authors.forename: Braulia C
authors.initials: BC
authors.lastname: Caetano
authors.forename: Cláudia M
authors.initials: CM
authors.lastname: Carneiro
authors.forename: Letícia A
authors.initials: LA
authors.lastname: Silva
authors.forename: Ricardo W A
authors.initials: RW
authors.lastname: Vitor
mesh_terms: D000818:Animals; D001681:Biological Assay; D001921:Brain / Q000469:parasitology / Q000473:pathology; D002478:Cells, Cultured; D002645:Chickens; D016207:Cytokines / Q000032:analysis; D004285:Dogs; D005260:Female; D005838:Genotype; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012008:Recurrence; D013154:Spleen / Q000166:cytology / Q000276:immunology; D014122:Toxoplasma / Q000145:classification / Q000235:genetics / Q000276:immunology*; D014124:Toxoplasmosis, Animal / Q000276:immunology*
chemical_list: D016207:Cytokines