title: Detection of proliferative responses to ESAT-6 and CFP-10 by FASCIA assay for diagnosis of Mycobacterium tuberculosis infection.
abstract: There is a large and growing worldwide need for reliable tests to diagnose active and latent tuberculosis (TB). Improved methodology for identifying individuals with true latent TB (LTBI), particularly those with a recent infection, would pave the way for targeted prophylactic treatment. The traditionally used tuberculin skin test (TST) is unspecific and impractical. Interferon gamma release assays (IGRA) are more specific than the TST but, like that test, cannot discriminate either between recent and remote TB infection, or between these and a mere immunological memory of previous TB infection. The Flow-cytometric Assay for Specific Cell-mediated Immune-response in Activated whole blood (FASCIA) combines long-term antigen stimulation of whole blood and flow-cytometric analysis with quantification of the expanded T-lymphoblasts and can also be employed for measurement of cytokine responses.
authors.affiliation: Unit of Infectious Diseases, Institution of Medicine, Karolinska Institutet, Karolinska University Hospital, Solna, 171 77 Stockholm, Sweden. emilie.wahren-borgstrom@karolinska.se
authors.forename: Emilie
authors.initials: E
authors.lastname: Borgström
authors.forename: Peter
authors.initials: P
authors.lastname: Andersen
authors.forename: Lena
authors.initials: L
authors.lastname: Andersson
authors.forename: Inger
authors.initials: I
authors.lastname: Julander
authors.forename: Gunilla
authors.initials: G
authors.lastname: Källenius
authors.forename: Markus
authors.initials: M
authors.lastname: Maeurer
authors.forename: Maria
authors.initials: M
authors.lastname: Norrby
authors.forename: Ida
authors.initials: I
authors.lastname: Rosenkrands
authors.forename: Teghesti
authors.initials: T
authors.lastname: Tecleab
authors.forename: Judith
authors.initials: J
authors.lastname: Bruchfeld
authors.forename: Hans
authors.initials: H
authors.lastname: Gaines
mesh_terms: D000328:Adult; D000368:Aged; D000942:Antigens, Bacterial / Q000032:analysis*; D001426:Bacterial Proteins / Q000032:analysis*; D049109:Cell Proliferation; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000737:chemistry* / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology; D014376:Tuberculosis / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D055815:Young Adult
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C412542:CFP 6 protein, Mycobacterium tuberculosis; C093276:ESAT-6 protein, Mycobacterium tuberculosis