title: Production of offspring from cloned transgenic RFP female dogs and stable generational transmission of the RFP gene.
abstract: The purpose of this study was to analyze the reproductive ability of transgenic female dogs born bysomatic cell nuclear transfer and to determine inheritance of the red fluorescent protein (RFP) transgene. The four founder transgenic bitches (F0) reached puberty at 340.8 ± 39.6 days after birth and were bred with wild-type male dogs by natural mating or by artificial insemination. The bitches all became pregnant and successfully delivered 13 puppies (F1), of which two females were bred with wild-type dogs to deliver 7 offspring (F2), including 1 stillbirth. Among the 19 live offspring, 10 puppies showed emission of RFP under UV light and the presence of the RFP transgene was confirmed by genomic PCR and Southern blot analyses. In conclusion, transgenic RFP female dogs exhibited normal reproductive ability and expression of the transgene was demonstrated in F1 and F2 generations.
authors.forename: So Gun
authors.initials: SG
authors.lastname: Hong
authors.forename: Hyun Ju
authors.initials: HJ
authors.lastname: Oh
authors.forename: Jung Eun
authors.initials: JE
authors.lastname: Park
authors.forename: Min Jung
authors.initials: MJ
authors.lastname: Kim
authors.forename: Geon A
authors.initials: GA
authors.lastname: Kim
authors.forename: Eun Jung
authors.initials: EJ
authors.lastname: Park
authors.forename: Ok Jae
authors.initials: OJ
authors.lastname: Koo
authors.forename: Sung Keun
authors.initials: SK
authors.lastname: Kang
authors.forename: Goo
authors.initials: G
authors.lastname: Jang
authors.forename: Byeong Chun
authors.initials: BC
authors.lastname: Lee
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics* / Q000378:metabolism; D026501:Blotting, Southwestern; D019976:Cloning, Organism / Q000379:methods*; D004285:Dogs / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005453:Fluorescence; D018014:Gene Transfer Techniques; D007315:Insemination, Artificial / Q000379:methods; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011374:Progesterone / Q000097:blood; D012098:Reproduction; D019076:Transgenes; D000097573:Red Fluorescent Protein
chemical_list: D008164:Luminescent Proteins; D011374:Progesterone; D000097573:Red Fluorescent Protein