title: Polymer brushes showing non-fouling in blood plasma challenge the currently accepted design of protein resistant surfaces.
abstract: Ultra-low-fouling poly[N-(2-hydroxypropyl) methacrylamide] (poly(HPMA)) brushes have been synthesized for the first time. Similar to the so far only ultra-low-fouling surface, poly(carboxybetaine acrylamide), the level of blood plasma fouling was below the detection limit of surface plasmon resonance (SPR, 0.03 ng·cm(-2)) despite being a hydrogen bond donor and displaying a moderate wettability, thus challenging the currently accepted views for the design of antifouling properties. The antifouling properties were preserved even after two years of storage. To demonstrate the potential of poly(HPMA) brushes for the preparation of bioactive ultra-low fouling surfaces a label-free SPR immunosensor for detection of G Streptococcus was prepared.
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, v.v.i., Prague, 162 00, Czech Republic. rodriguez@imc.cas.cz
authors.forename: Cesar
authors.initials: C
authors.lastname: Rodriguez-Emmenegger
authors.forename: Eduard
authors.initials: E
authors.lastname: Brynda
authors.forename: Tomas
authors.initials: T
authors.lastname: Riedel
authors.forename: Milan
authors.initials: M
authors.lastname: Houska
authors.forename: Vladimir
authors.initials: V
authors.lastname: Šubr
authors.forename: Aldo Bologna
authors.initials: AB
authors.lastname: Alles
authors.forename: Erol
authors.initials: E
authors.lastname: Hasan
authors.forename: Julien E
authors.initials: JE
authors.lastname: Gautrot
authors.forename: Wilhelm T S
authors.initials: WT
authors.lastname: Huck
mesh_terms: D000327:Adsorption; D015374:Biosensing Techniques / Q000295:instrumentation*; D001798:Blood Proteins / Q000737:chemistry*; D006801:Humans; D011109:Polymethacrylic Acids / Q000138:chemical synthesis / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D001798:Blood Proteins; D011109:Polymethacrylic Acids; C011533:Duxon