title: Rapid screening of complex DNA samples by single-molecule amplification and sequencing.
abstract: Microbial cloning makes Sanger sequencing of complex DNA samples possible but is labor intensive. We present a simple, rapid and robust method that enables laboratories without special equipment to perform single-molecule amplicon sequencing, although in a low-throughput manner, from sub-picogram quantities of DNA. The method can also be used for quick quality control of next-generation sequencing libraries, as was demonstrated for a metagenomic sample.
authors.affiliation: Department of Medical Epidemiology and Biostatistics, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Jiaqi
authors.initials: J
authors.lastname: Huang
authors.forename: Zongli
authors.initials: Z
authors.lastname: Zheng
authors.forename: Anders F
authors.initials: AF
authors.lastname: Andersson
authors.forename: Lars
authors.initials: L
authors.lastname: Engstrand
authors.forename: Weimin
authors.initials: W
authors.lastname: Ye
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics*; D056186:Metagenomics; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial