title: Templated construction of a Zn-selective protein dimerization motif.
abstract: Here, we report that the approach of metal-templated ligand synthesis can be applied to construct a dimeric protein assembly ((BMOE)RIDC1(2)), which is stabilized by noncovalent interactions and flexible covalent cross-linkers around the Zn templates. Despite its flexibility, (BMOE)RIDC1(2) selectively binds Zn(II) over other divalent metals and undergoes dimerization upon metal binding. Such simultaneous fulfillment of plasticity and selectivity is a hallmark of cellular signaling events that involve ligand/metal-induced protein dimerization.
authors.affiliation: Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, MC 0356, La Jolla, California 92093, USA.
authors.forename: Eric N
authors.initials: EN
authors.lastname: Salgado
authors.forename: Jeffrey D
authors.initials: JD
authors.lastname: Brodin
authors.forename: Magnus M
authors.initials: MM
authors.lastname: To
authors.forename: F Akif
authors.initials: FA
authors.lastname: Tezcan
mesh_terms: D045304:Cytochromes c / Q000737:chemistry* / Q000378:metabolism; D008024:Ligands; D008958:Models, Molecular; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D055503:Protein Multimerization; D015032:Zinc / Q000737:chemistry*
chemical_list: D008024:Ligands; D009942:Organometallic Compounds; D045304:Cytochromes c; D015032:Zinc