title: Capillary Electrokinetic Chromatography Employing p-(Carboxyethyl)calix[n]arenes as Running Buffer Additives.
abstract: Calixarenes, a class of macrocyclic phenolic compounds with a basket-like shape, are used as capillary electrophoresis reagents for separations of native and substituted polycyclic aromatic hydrocarbons. The p-(carboxyethyl)calix[n]arenes reported herein are a series of charged, moderately water soluble macrocyclic molecules that can form complexes with neutral molecules. Electrokinetic chromatographic separations are based on the differential distribution of molecules between a running buffer phase, which is transported by electroosmotic flow, and an electrophoretically mediated calixarene. The size of the calixarene influences separation performance, illustrating the importance of cavity size and geometry in the complexation process. p-(Carboxyethyl)calix[7]arene provides the best efficiency (>10(5) plates/m) and selectivity in these studies. The influences of pH, organic solvent, and field strength on elution range, capacity factors, efficiency, and selectivity are also reported. In general, capacity factors are rather low, but the high charge-to-mass ratios of certain calixarenes produce relatively wide elution ranges. Molecular modeling data and solubility data are used to interpret the observed selectivity.
authors.affiliation: Department of Chemistry, University of Tennessee, Knoxville Tennessee 37996-1600.
authors.forename: S
authors.initials: S
authors.lastname: Sun
authors.forename: M J
authors.initials: MJ
authors.lastname: Sepaniak
authors.forename: J S
authors.initials: JS
authors.lastname: Wang
authors.forename: C D
authors.initials: CD
authors.lastname: Gutsche