title: High glucose induced rat aorta vascular smooth muscle cell oxidative injury: involvement of protein tyrosine nitration.
abstract: The alteration and further damage of vascular smooth muscle function have been implicated in the development of vascular complications and diabetes. Little is known about protein tyrosine nitration in vascular smooth muscle cell injury induced by high glucose. In this article, vascular smooth muscle cell was exposed to 30 and 40 mM high glucose for 72 h, and then the cell injury in vascular smooth muscle cell induced by high glucose was studied. It was found that high glucose stimulated vascular smooth muscle cell injury in a dose-dependent manner, including decreasing intracellular and extracellular glutathione contents, increasing malondialdehyde and intracellular reactive oxygen species content, increasing the production of nitric oxide (increased nitrite content in cell and medium), as well as increasing protein tyrosine nitration. By comparing protein tyrosine nitration induced by high glucose conditions and extrinsic factors (hemin-nitrite-glucose oxidase system and 3-morpholinosydnonimine), it may be speculated that protein is nitrated selectively, and specific protein tyrosine nitration is involved in diabetic vascular complications.
authors.affiliation: College of Chemical and Life Science, Zhejiang Normal University, Jinhua, 321004, People's Republic of China.
authors.forename: Yuling
authors.initials: Y
authors.lastname: Zhao
authors.forename: Naihao
authors.initials: N
authors.lastname: Lu
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zhonghong
authors.initials: Z
authors.lastname: Gao
mesh_terms: D000818:Animals; D001011:Aorta / Q000187:drug effects / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D005947:Glucose / Q000008:administration & dosage*; D005949:Glucose Oxidase / Q000008:administration & dosage; D005978:Glutathione / Q000378:metabolism; D006427:Hemin / Q000008:administration & dosage; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008981:Molsidomine / Q000008:administration & dosage / Q000031:analogs & derivatives; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D032389:Myocytes, Smooth Muscle / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D009569:Nitric Oxide / Q000008:administration & dosage; D061251:Primary Cell Culture; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism*; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000187:drug effects / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D009569:Nitric Oxide; C002744:3-nitrotyrosine; D014443:Tyrosine; C002385:linsidomine; D006427:Hemin; D008981:Molsidomine; D005949:Glucose Oxidase; D005978:Glutathione; D005947:Glucose