title: Flow-alignment of bicellar lipid mixtures: orientations of probe molecules and membrane-associated biomacromolecules in lipid membranes studied with polarized light.
abstract: Bicelles are excellent membrane-mimicking hosts for a dynamic and structural study of solutes with NMR, but the magnetic fields required for their alignment are hard to apply to optical conditions. Here we demonstrate that bicellar mixtures can be aligned by shear forces in a Couette flow cell, to provide orientation of membrane-bound retinoic acid, pyrene and cytochrome c (cyt c) protein, conveniently studied with linear dichroism spectroscopy.
authors.affiliation: Department of Chemical and Biological Engineering, Physical Chemistry, Chalmers University of Technology, SE-412 96 Gothenburg, Sweden.
authors.forename: Maxim
authors.initials: M
authors.lastname: Kogan
authors.forename: Tamás
authors.initials: T
authors.lastname: Beke-Somfai
authors.forename: Bengt
authors.initials: B
authors.lastname: Nordén
mesh_terms: D002462:Cell Membrane / Q000737:chemistry*; D008027:Light; D008051:Lipid Bilayers / Q000737:chemistry*; D055620:Optical Phenomena; D011721:Pyrenes / Q000737:chemistry*; D013696:Temperature; D014212:Tretinoin / Q000737:chemistry*
chemical_list: D008051:Lipid Bilayers; D011721:Pyrenes; D014212:Tretinoin; C030984:pyrene