title: Effects of temporarily disrupting BBB on activity-induced manganese-dependent functional MRI.
abstract: This study further investigates the influence of temporarily disrupting the blood-brain barrier (BBB) on the level of manganese used in AIM fMRI other than the recognized function of allowing that substance to enter into the activated brain regions more effectively during the BBB opening. We injected manganese into Wistar rats through ICA following the disruption of BBB with mannitol in a functional MRI test of the visual cortex. Through comparing MRI signal intensity and manganese contents in the visual cortex of rats received visual stimuli of unequal degree after the restoration of BBB, we found that the signal in the visual cortex could be further enhanced on T1WI given visual stimulation after the restoration of BBB. Temporary BBB disruption has an additional advantage in allowing Mn(2+) to enter the CSF or brain for later transference to the activated brain area. So the dosage of manganese in AIM fMRI could be minimized by extending the stimulus.
authors.affiliation: Department of Neurosurgery, Neurosurgery Institute, Key Laboratory on Brain Function Repair and Regeneration of Guangdong, Zhujiang Hospital of Southern Medical University, Guangzhou, People's Republic of China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Fa
authors.forename: Run
authors.initials: R
authors.lastname: Zhang
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.forename: Shaofang
authors.initials: S
authors.lastname: Zhu
authors.forename: Qu
authors.initials: Q
authors.lastname: Wu
authors.forename: Fanheng
authors.initials: F
authors.lastname: Huang
authors.forename: Yanping
authors.initials: Y
authors.lastname: Liu
authors.forename: Lujun
authors.initials: L
authors.lastname: Yang
authors.forename: Haigang
authors.initials: H
authors.lastname: Chang
authors.forename: Zhibo
authors.initials: Z
authors.lastname: Wen
authors.forename: Dingguo
authors.initials: D
authors.lastname: Gao
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Zeng
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Jiang
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier / Q000502:physiology*; D003627:Data Interpretation, Statistical; D004232:Diuretics / Q000494:pharmacology; D007089:Image Enhancement; D007091:Image Processing, Computer-Assisted; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D008345:Manganese / Q000134:cerebrospinal fluid / Q000378:metabolism / Q000502:physiology*; D008353:Mannitol / Q000494:pharmacology; D010775:Photic Stimulation; D051381:Rats; D017208:Rats, Wistar; D014793:Visual Cortex / Q000502:physiology; D014794:Visual Fields
chemical_list: D004232:Diuretics; D008353:Mannitol; D008345:Manganese