title: Drought, salt and wounding stress induce the expression of the plasma membrane intrinsic protein 1 gene in poplar (Populus alba×P. tremula var. glandulosa).
abstract: Water uptake across cell membranes is a principal requirement for plant growth at both the cellular and whole-plant levels; water movement through plant membranes is regulated by aquaporins (AQPs) or major intrinsic proteins (MIPs). We examined the expression characteristics of the poplar plasma membrane intrinsic protein 1 gene (PatPIP1), a type of MIP, which was isolated from a suspension cell cDNA library of Populus alba×P. tremula var. glandulosa. Examination of protoplasts expressing the p35S-PatPIP1::sGFP fusion protein revealed that the protein was localized in the plasma membrane. Northern blot analysis revealed that the gene was strongly expressed in poplar roots and leaves. Gene expression was inducible by abiotic factors including drought, salinity, cold temperatures and wounding, and also by plant hormones including gibberellic acid, jasmonic acid and salicylic acid. Since we found that the PatPIP1 gene was strongly expressed in response to mannitol, NaCl, jasmonic acid and wounding, we propose that PatPIP1 plays an essential role in the defense of plants against water stress.
authors.affiliation: Biotechnology Division, Korea Forest Research Institute, Suwon, Republic of Korea.
authors.forename: Eun-Kyung
authors.initials: EK
authors.lastname: Bae
authors.forename: Hyoshin
authors.initials: H
authors.lastname: Lee
authors.forename: Jae-Soon
authors.initials: JS
authors.lastname: Lee
authors.forename: Eun-Woon
authors.initials: EW
authors.lastname: Noh
mesh_terms: D000595:Amino Acid Sequence; D020346:Aquaporins / Q000235:genetics* / Q000378:metabolism; D003080:Cold Temperature; D003681:Dehydration / Q000235:genetics; D055864:Droughts; D018745:Genome, Plant; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D032107:Populus / Q000235:genetics*; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological; D014947:Wounds and Injuries / Q000235:genetics
chemical_list: D020346:Aquaporins; D010940:Plant Proteins; C110065:major intrinsic protein, plant; D012965:Sodium Chloride