title: HIF-1α ODD polypeptides increased the expression of HIF1 and VEGF in hypoxic rat cortical neuron.
abstract: To investigate the effect of HIF-1α ODD polypeptides on the expression of HIF-1α and VEGF in primary rat cortical neuron in hypoxia. Primary cultured neurons were exposed to hypoxia for 2, 4, and 8 h. Tat-ODD fusion polypeptide was added before hypoxia in the experimental groups. The expression of HIF-1α and VEGF was detected by Western blot and real time PCR. The levels of HIF-1α and VEGF peaked at 4 h then declined in hypoxia. The expression of HIF-1α and VEGF was increased by tat-ODD fusion polypeptide. tat-ODD fusion polypeptide might improve HIF1 adaptation to hypoxia by increased expression of HIF-1α and VEGF.
authors.affiliation: Department of Obstetrics and Gynecology, Shengjing Hospital, China Medical University, Shenyang, China.
authors.forename: Xuemin
authors.initials: X
authors.lastname: Liu
authors.forename: Juan
authors.initials: J
authors.lastname: Du
authors.forename: Qi
authors.initials: Q
authors.lastname: Xi
mesh_terms: D000818:Animals; D015687:Cell Hypoxia / Q000187:drug effects* / Q000502:physiology; D002540:Cerebral Cortex / Q000166:cytology*; D051793:Hypoxia-Inducible Factor 1 / Q000737:chemistry / Q000378:metabolism; D009474:Neurons / Q000187:drug effects*; D010455:Peptides / Q000494:pharmacology*; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
chemical_list: D051793:Hypoxia-Inducible Factor 1; D010455:Peptides; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A