title: Screening, identification and quantification of glucosinolates in black radish (Raphanus sativus L. niger) based dietary supplements using liquid chromatography coupled with a photodiode array and liquid chromatography-mass spectrometry.
abstract: The glucosinolate profile of black radish (Raphanus sativus L. niger) based dietary supplements has been investigated by HPLC-PDA, LC-ESI-MS/MS and LC-APCI-MS/MS systems. Optimization of the MS/MS parameters and LC conditions was performed using sinigrin reference standard and rapeseed certified reference material (BC190) respectively. An LC-ESI-MS/MS system was used to detect (screen) and identify the naturally occurring intact glucosinolates (GLs). The intact GLs identified were then desulfated and quantified on an HPLC-PDA system as desulfo-glucosinolates (DS-GLs). Prior to quantification, the DS-GLs were identified using an APCI-MS/MS. The HPLC-PDA method performance criteria were evaluated using glucotropaeolin potassium salt. The validated method was applied for the analysis of six dietary supplements. In total, six glucosinolates were identified and quantified in the dietary supplements; glucoraphasatin (0.2-0.48 mg/g), glucosisaustricin (0.37-0.91 mg/g), glucoraphenin (0.84-1.27 mg/g), glucoputrajivin (0.14-0.28 mg/g), glucosisymbrin (0.70-0.99 mg/g) and gluconasturtiin (0.06-0.12 mg/g). Glucoraphenin was the most abundant glucosinolate in all samples.
authors.affiliation: Laboratory of Food Analysis, Faculty of Pharmaceutical Sciences, Ghent University, Ghent, Belgium. emmanuel.njumbeediage@ugent.be
authors.forename: E Njumbe
authors.initials: EN
authors.lastname: Ediage
authors.forename: J Diana
authors.initials: JD
authors.lastname: Di Mavungu
authors.forename: M L
authors.initials: ML
authors.lastname: Scippo
authors.forename: Y J
authors.initials: YJ
authors.lastname: Schneider
authors.forename: Y
authors.initials: Y
authors.lastname: Larondelle
authors.forename: A
authors.initials: A
authors.lastname: Callebaut
authors.forename: J
authors.initials: J
authors.lastname: Robbens
authors.forename: C
authors.initials: C
authors.lastname: Van Peteghem
authors.forename: S
authors.initials: S
authors.lastname: De Saeger
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019587:Dietary Supplements / Q000032:analysis*; D005961:Glucosinolates / Q000032:analysis* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D031224:Raphanus / Q000737:chemistry*; D012044:Regression Analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D005961:Glucosinolates