title: Preventive effect of chlorogenic acid on lens opacity and cytotoxicity in human lens epithelial cells.
abstract: The present study reveals the pharmacological effect of chlorogenic acid, a major phenolic compound in plants, food, and coffee, on diabetic cataracts. Chlorogenic acid examined the inhibitory effects upon rat lens aldose reductase (AR) activity and xylose-induced rat lens opacity. The effect of chlorogenic acid on high glucose-induced cytotoxicity in lens epithelial cells was also examined. Chlorogenic acid showed potential inhibitory activity against rat lens AR, with an IC₅₀ value of 0.95 µM. The xylose-induced opacity of lenses was significantly improved after treatment with chlorogenic acid in a dose-dependent manner. Chlorogenic acid prevented high glucose-induced cytotoxicity in human lens epithelial (HLE-B3) cells in a dose-dependent manner. These results suggest that chlorogenic acid may provide a potential therapeutic approach for prevention of diabetic complications, such as cataracts.
authors.affiliation: Diabetic Complications Research Center, Division of Traditional Korean Medicine (TKM) Integrated Research, Korea Institute of Oriental Medicine (KIOM), Daejeon, Korea.
authors.forename: Young Sook
authors.initials: YS
authors.lastname: Kim
authors.forename: Nan Hee
authors.initials: NH
authors.lastname: Kim
authors.forename: Yun Mi
authors.initials: YM
authors.lastname: Lee
authors.forename: Jin Sook
authors.initials: JS
authors.lastname: Kim
mesh_terms: D000449:Aldehyde Reductase / Q000037:antagonists & inhibitors; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002386:Cataract / Q000139:chemically induced / Q000209:etiology / Q000517:prevention & control*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002726:Chlorogenic Acid / Q000494:pharmacology*; D048909:Diabetes Complications / Q000517:prevention & control; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004847:Epithelial Cells / Q000187:drug effects*; D006801:Humans; D006943:Hyperglycemia / Q000503:physiopathology; D007908:Lens, Crystalline / Q000187:drug effects* / Q000473:pathology; D009924:Organ Culture Techniques; D009994:Osmolar Concentration; D051381:Rats; D017207:Rats, Sprague-Dawley; D014994:Xylose / Q000633:toxicity
chemical_list: D000975:Antioxidants; D004791:Enzyme Inhibitors; D002726:Chlorogenic Acid; D014994:Xylose; D000449:Aldehyde Reductase