title: Single-embryo metabolomics and systematic prediction of developmental stage in zebrafish.
abstract: Metabolites, the end products of gene expression in living organisms, are tightly correlated with an organism's development and growth. Thus, metabolic profiling is a potentially important tool for understanding the events that have occurred in cells, tissues, and individual organisms. Here, we present a method for predicting the developmental stage of zebrafish embryos using novel metabolomic non-target fingerprints of "single-embryos". With this method, we observed the rate of development at different temperatures. Our results suggest that this method allows us to analyse the condition, or distinguish the genotype, of single-embryos before expression of their ultimate phenotype.
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Osaka University, Yamadaoka, Suita 565-0871, Japan.
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Hayashi
authors.forename: Mako
authors.initials: M
authors.lastname: Yoshida
authors.forename: Toshinobu
authors.initials: T
authors.lastname: Fujiwara
authors.forename: Shingo
authors.initials: S
authors.lastname: Maegawa
authors.forename: Eiichiro
authors.initials: E
authors.lastname: Fukusaki
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid; D004625:Embryo, Nonmammalian / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D013058:Mass Spectrometry; D055432:Metabolomics; D015999:Multivariate Analysis; D015027:Zebrafish / Q000196:embryology*