title: Effects of different elicitors on yield of tropane alkaloids in hairy roots of Anisodus acutangulus.
abstract: The four tropane alkaloids have played a pivotal role in controlling diseases such as the toxic and septic shock, the organophosphorus poison and the acute lung injury. Here, the elicitation effect of different elicitors on the production of tropane alkaloids and the molecular mechanism of enzyme genes in the pathway was firstly demonstrated in hairy roots of Anisodus acutangulus. The results showed ethanol, methyl jasmonate and Ag(+) could improve the accumulation of tropane alkaloids up to 1.51, 1.13 and 1.08 times after 24 h treatment, respectively (P < 0.05), whereas salicylic acid decreased the average content of tropane alkaloids. Furthermore, expression profile analysis results revealed that up-regulation of hyoscyamine-6b-hydroxylase (AaH6H) and little regulation of tropinone reducase II (AaTR2) elicited by ethanol, increased expression of putrescine N-methyltransferase I (AaPMT1) elicited by Ag(+), elevated expression of tropinone reducase I (AaTR1) elicited by methyl jasmonate, respectively, resulted in tropane alkaloids improvement. Our results showed that hairy root culture of A. acutangulus in combination with elicitors was a promising way for production of tropane alkaloids in the future.
authors.affiliation: Laboratory of Plant Biotechnology, College of Life and Environment Sciences, Shanghai Normal University, Shanghai 200234, People's Republic of China. gykai@shnu.edu.cn
authors.forename: Guoyin
authors.initials: G
authors.lastname: Kai
authors.forename: Sheng
authors.initials: S
authors.lastname: Yang
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xiuqin
authors.initials: X
authors.lastname: Luo
authors.forename: Xueqing
authors.initials: X
authors.lastname: Fu
authors.forename: Ang
authors.initials: A
authors.lastname: Zhang
authors.forename: Jianbo
authors.initials: J
authors.lastname: Xiao
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D000704:Analysis of Variance; D002851:Chromatography, High Pressure Liquid; D003517:Cyclopentanes / Q000494:pharmacology*; D017931:DNA Primers / Q000235:genetics; D000431:Ethanol / Q000494:pharmacology*; D054883:Oxylipins / Q000494:pharmacology*; D018517:Plant Roots / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020156:Salicylic Acid / Q000494:pharmacology*; D012834:Silver / Q000494:pharmacology*; D019657:Solanaceae / Q000378:metabolism*; D013997:Time Factors; D014326:Tropanes / Q000378:metabolism*
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D017931:DNA Primers; D054883:Oxylipins; D014326:Tropanes; D000431:Ethanol; D012834:Silver; C072239:methyl jasmonate; D020156:Salicylic Acid