title: Comparison of the in vivo distribution of four different annexin a5 adducts in rhesus monkeys.
abstract: Annexin A5 has been used for the detection of apoptotic cells, due to its ability to bind to phosphatidylserine (PS). Four different labeled Annexin A5 adducts were evaluated in rhesus monkey, with radiolabeling achieved via 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA). Of these adducts differing conjugation methods were employed which resulted in nonspecific radiolabeling (AxA5-I), or site-specific radiolabeling (AxA5-II). A nonbinding variant of Annexin A5 was also evaluated (AxA5-II(NBV)), conjugation here was site specific. The fourth adduct examined had both specific and nonspecific conjugation techniques employed (AxA5-II(mDOTA)). Blood clearance for each adduct was comparable, while appreciable uptake was observed in kidney, liver, and spleen. Significant differences in uptake of AxA5-I and AxA5-II were observed, as well as between AxA5-II and AxA5-II(NBV). No difference between AxA5-II and AxA5-II(mDOTA) was observed, suggesting that conjugating DOTA nonspecifically did not affect the in vivo biodistribution of Annexin A5.
authors.affiliation: Imaging Research, Merck Research Laboratories, West Point, PA 19486, USA.
authors.forename: Paul
authors.initials: P
authors.lastname: McQuade
authors.forename: Marie-Jose
authors.initials: MJ
authors.lastname: Belanger
authors.forename: Xiangjun
authors.initials: X
authors.lastname: Meng
authors.forename: Ilonka
authors.initials: I
authors.lastname: Guenther
authors.forename: Stephen
authors.initials: S
authors.lastname: Krause
authors.forename: Dinko
authors.initials: D
authors.lastname: Gonzalez Trotter
authors.forename: Chris
authors.initials: C
authors.lastname: Reutelingsperger
authors.forename: Eric
authors.initials: E
authors.lastname: Hostetler
authors.forename: Michael
authors.initials: M
authors.lastname: Klimas
authors.forename: Huseyin
authors.initials: H
authors.lastname: Mehmet
authors.forename: Jacquelynn
authors.initials: J
authors.lastname: Cook