title: Polyvalent nucleic acid nanostructures.
abstract: Polyvalent oligonucleotide-nanoparticle conjugates possess several unique emergent properties, including enhanced cellular uptake, high antisense bioactivity, and nuclease resistance, which hypothetically originate from the dense packing and orientation of oligonucleotides on the surface of the nanoparticle. In this Communication, we describe a new class of polyvalent nucleic acid nanostructures (PNANs), which are comprised of only cross-linked and oriented nucleic acids. We demonstrate that these particles are capable of effecting high cellular uptake and gene regulation without the need of a cationic polymer co-carrier. The PNANs also exhibit cooperative binding behavior and nuclease resistance properties.
authors.affiliation: Department of Chemistry and International Institute for Nanotechnology, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208-3113, USA.
authors.forename: Joshua I
authors.initials: JI
authors.lastname: Cutler
authors.forename: Ke
authors.initials: K
authors.lastname: Zhang
authors.forename: Dan
authors.initials: D
authors.lastname: Zheng
authors.forename: Evelyn
authors.initials: E
authors.lastname: Auyeung
authors.forename: Andrew E
authors.initials: AE
authors.lastname: Prigodich
authors.forename: Chad A
authors.initials: CA
authors.lastname: Mirkin
mesh_terms: D001692:Biological Transport; D045744:Cell Line, Tumor; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D003851:Deoxyribonucleases / Q000378:metabolism; D006801:Humans; D049329:Nanostructures / Q000737:chemistry*; D012313:RNA / Q000737:chemistry* / Q000378:metabolism; D012260:Ribonucleases / Q000378:metabolism
chemical_list: D012313:RNA; D004247:DNA; D003851:Deoxyribonucleases; D012260:Ribonucleases