title: A novel gammaherpesvirus isolated from a black-tailed prairie dog (Cynomys ludovicianus).
abstract: A new gammaherpesvirus, tentatively named cynomys herpesvirus 1 (CynGHV-1), was isolated from a black-tailed prairie dog (Cynomys ludovicianus). CynGHV-1 replicated cytopathogenically to moderate titers in various cell lines. Ten kb of the CynGHV-1 genome was sequenced using degenerate PCR and genomic cloning. Sequence similarities were found to different genes from known gammaherpesviruses. Phylogenetic analysis suggested that CynGHV-1 was in fact a novel virus closely related to representatives of different genera and unclassified members of the subfamily Gammaherpesvirinae. However, CynGHV-1 could not be assigned to any particular genus and therefore remains unclassified.
authors.affiliation: Department of Veterinary Science, University of Wyoming, Laramie, WY 82070, USA.
authors.forename: Brandy
authors.initials: B
authors.lastname: Nagamine
authors.forename: Leandro
authors.initials: L
authors.lastname: Jones
authors.forename: Christian
authors.initials: C
authors.lastname: Tellgren-Roth
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Cavender
authors.forename: Ana C
authors.initials: AC
authors.lastname: Bratanich
mesh_terms: D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D006564:Herpesviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012589:Sciuridae / Q000821:virology*; D014779:Virus Replication