title: Competitive protein adsorption on polysaccharide and hyaluronate modified surfaces.
abstract: Adsorption of bovine serum albumin (BSA) and fibrinogen (Fg) was measured on six distinct bare and dextran- and hyaluronate-modified silicon surfaces created using two dextran grafting densities and three hyaluronic acid (HA) sodium salts derived from human umbilical cord, rooster comb and Streptococcus zooepidemicus. Film thickness and surface morphology depended on the HA molecular weight and concentration. BSA coverage was enhanced on surfaces in competitive adsorption of BSA:Fg mixtures. Dextranization differentially reduced protein adsorption onto surfaces based on oxidation state. Hyaluronization was demonstrated to provide the greatest resistance to protein coverage, equivalent to that of the most resistant dextranized surface. Resistance to protein adsorption was independent of the type of HA utilized. With changing bulk protein concentration from 20 to 40 μg ml(-1) for each species, Fg coverage on silicon increased by 4x, whereas both BSA and Fg adsorption on dextran and HA were far less dependent on protein bulk concentration.
authors.affiliation: Department of Anesthesiology and Critical Care, University of Pennsylvania, Philadelphia, PA 19104, USA.
authors.forename: Michela
authors.initials: M
authors.lastname: Ombelli
authors.forename: Lauren
authors.initials: L
authors.lastname: Costello
authors.forename: Corinne
authors.initials: C
authors.lastname: Postle
authors.forename: Vinod
authors.initials: V
authors.lastname: Anantharaman
authors.forename: Qing Cheng
authors.initials: QC
authors.lastname: Meng
authors.forename: Russell J
authors.initials: RJ
authors.lastname: Composto
authors.forename: David M
authors.initials: DM
authors.lastname: Eckmann
mesh_terms: D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002417:Cattle; D002645:Chickens; D003129:Comb and Wattles / Q000378:metabolism*; D003911:Dextrans / Q000737:chemistry*; D005340:Fibrinogen / Q000737:chemistry*; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry*; D010084:Oxidation-Reduction; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012825:Silicon / Q000737:chemistry; D018502:Streptococcus equi / Q000378:metabolism*; D013499:Surface Properties; D014470:Umbilical Cord / Q000378:metabolism*
chemical_list: D001672:Biocompatible Materials; D003911:Dextrans; D012710:Serum Albumin, Bovine; D005340:Fibrinogen; D006820:Hyaluronic Acid; D012825:Silicon