title: Rapid confocal imaging of large areas of excised tissue with strip mosaicing.
abstract: Imaging large areas of tissue rapidly and with high resolution may enable rapid pathology at the bedside. The limited field of view of high-resolution microscopes requires the merging of multiple images that are taken sequentially to cover a large area. This merging or mosaicing of images requires long acquisition and processing times, and produces artifacts. To reduce both time and artifacts, we developed a mosaicing method on a confocal microscope that images morphology in large areas of excised tissue with sub-cellular detail. By acquiring image strips with aspect ratios of 10:1 and higher (instead of the standard ~1:1) and "stitching" them in software, our method images 10 × 10 mm(2) area of tissue in about 3 min. This method, which we call "strip mosaicing," is currently three times as fast as our previous method.
authors.affiliation: Memorial Sloan-Kettering Cancer Center, Research Engineering Laboratory, 430 East 67th Street, RRL513, New York, New York 10065, USA.
authors.forename: Sanjee
authors.initials: S
authors.lastname: Abeytunge
authors.forename: Yongbiao
authors.initials: Y
authors.lastname: Li
authors.forename: Bjorg
authors.initials: B
authors.lastname: Larson
authors.forename: Ricardo
authors.initials: R
authors.lastname: Toledo-Crow
authors.forename: Milind
authors.initials: M
authors.lastname: Rajadhyaksha
mesh_terms: D000465:Algorithms; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D007089:Image Enhancement / Q000295:instrumentation*; D018613:Microscopy, Confocal / Q000295:instrumentation*; D018715:Microscopy, Video / Q000295:instrumentation*; D015580:Mohs Surgery / Q000295:instrumentation*; D010340:Pathology, Surgical / Q000295:instrumentation*; D010363:Pattern Recognition, Automated / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012878:Skin Neoplasms / Q000473:pathology*