title: Anti-complementary ginsenosides isolated from processed ginseng.
abstract: As part of an ongoing search for immunomodulatory components aimed at the anti-complementary effect, ginsenosides isolated from processed ginseng were found to have inhibitory activity on complement activation through classical pathways. Activity-guided fractionation was used to isolate four ginsenosides, namely ginsenoside Rg₆, F₄, Rk₃, and Rh₄. Ginsenoside Rk₃ and Rh₄ had a 3 fold higher inhibition activity than rosmarinic acid which was used as a positive control while ginsenoside Rg₆ and F₄ showed only mild effects similar to that of the positive control. The results suggest that the activity of the corresponding ginsenosides may be increased by the glycosyl moiety at the C₆ position rather than the double bond conformation at C₂₀, and ginsenoside Rk₃ and Rh₄ could have a role in treating inflammatory diseases.
authors.affiliation: College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul, Korea.
authors.forename: Jin Gyun
authors.initials: JG
authors.lastname: Lee
authors.forename: Seung Hoon
authors.initials: SH
authors.lastname: Baek
authors.forename: Yong Yook
authors.initials: YY
authors.lastname: Lee
authors.forename: Seo Young
authors.initials: SY
authors.lastname: Park
authors.forename: Jeong Hill
authors.initials: JH
authors.lastname: Park
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000302:isolation & purification / Q000494:pharmacology; D005591:Chemical Fractionation; D003168:Complement Fixation Tests; D051056:Complement Inactivating Agents / Q000302:isolation & purification* / Q000494:pharmacology*; D003171:Complement Pathway, Classical / Q000187:drug effects*; D004912:Erythrocytes / Q000187:drug effects; D036145:Ginsenosides / Q000302:isolation & purification* / Q000494:pharmacology*; D006801:Humans; D009994:Osmolar Concentration; D005894:Panax / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry*; D012756:Sheep
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D051056:Complement Inactivating Agents; D036145:Ginsenosides; C101280:ginsenoside Rh4; C472075:ginsenoside Rk3