title: Comment on "A bacterium that can grow by using arsenic instead of phosphorus".
abstract: Wolfe-Simon et al. (Research Articles, 3 June 2011, p. 1163; published online 2 December 2010) reported that a naturally occurring bacterium, strain GFAJ-1, can substitute arsenic for phosphorus in its biomolecules. However, straightforward experiments to support this claim, including density gradient centrifugation of DNA assumed to contain arsenic, were either not performed or not presented. As a result, the authors' conclusions remain uncertain.
authors.affiliation: Biomedical Sciences Research Center Alexander Fleming, 16672 Vari, Greece. oehler@fleming.gr
authors.forename: Stefan
authors.initials: S
authors.lastname: Oehler
mesh_terms: D001149:Arsenates / Q000378:metabolism; D001151:Arsenic / Q000032:analysis / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry* / Q000378:metabolism; D044129:Halomonadaceae / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism; D010758:Phosphorus / Q000378:metabolism*; D012107:Research Design
chemical_list: D001149:Arsenates; D003470:Culture Media; D004269:DNA, Bacterial; D010710:Phosphates; D010758:Phosphorus; D001151:Arsenic; C025657:arsenic acid