title: A Bowman-Birk inhibitor with anti-elastase activity from Lathyrus sativus L. seeds.
abstract: Four Bowman-Birk inhibitors, named LSI-1/4, were isolated and purified from Lathyrus sativus L. seeds. The purification procedure consisted of two cation-exchange chromatography steps, followed by gel-filtration and RP-HPLC. Mass spectrometry analysis of LSI-1/4 inhibitors yielded relative molecular masses of 7914.41 for LSI-1, 6867.67 for LSI-2, 7341.24 for LSI-3 and 7460.01 for LSI-4. N-terminal sequences (up to 30 residues) of LSI-1/4 inhibitors were identical with the exception of sequence positions 21, 27 and 28 and highly similar to those of other Bowman-Birk inhibitors isolated from Leguminosae plants. Inhibitors LSI-1/4 were active towards trypsin and α-chymotrypsin, with IC(50) values for 12.6 nM of trypsin ranging from 4.9 to 24.3 nM. A lower activity was observed against bovine α-chymotrypsin (IC(50) values ranging from 0.5 to 3.4 μM for 15.0 nM of α-chymotrypsin). Peptide mapping of the LSI-1 sequence showed the presence of an Ala residue in the second reactive site, thus explaining the low anti-chymotrypsin activity of this inhibitor. In addition, LSI-1 was endowed with anti-elastase activity, being able to inhibit human leukocyte elastase.
authors.affiliation: Dipartimento di Scienze della Vita, Seconda Università degli Studi di Napoli, Caserta, Italy.
authors.forename: Micaela
authors.initials: M
authors.lastname: Rocco
authors.forename: Livia
authors.initials: L
authors.lastname: Malorni
authors.forename: Angela
authors.initials: A
authors.lastname: Chambery
authors.forename: Elia
authors.initials: E
authors.lastname: Poerio
authors.forename: Augusto
authors.initials: A
authors.lastname: Parente
authors.forename: Antimo
authors.initials: A
authors.lastname: Di Maro
mesh_terms: D000595:Amino Acid Sequence; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D027807:Lathyrus / Q000737:chemistry*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors*; D010449:Peptide Mapping; D012639:Seeds / Q000737:chemistry; D016415:Sequence Alignment; D014358:Trypsin Inhibitor, Bowman-Birk Soybean / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D014361:Trypsin Inhibitors / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology
chemical_list: D014358:Trypsin Inhibitor, Bowman-Birk Soybean; D014361:Trypsin Inhibitors; D010196:Pancreatic Elastase