title: Validation of a method for the analysis of nine quinolones in eggs by pressurized liquid extraction and liquid chromatography with fluorescence detection.
abstract: A multiresidue method for the analysis, in egg matrices, of residues of nine quinolones used in veterinary medicine, has been developed and validated according to the provisions of Council Decision 2002/657. Compounds were extracted by a pressurized liquid extraction (PLE) technique using a 1:1 mixture of acetonitrile and a phosphoric acid buffer (pH 3.0) at 70°C. The obtained extract was clear enough, so that no further clean-up was necessary. Analytes were determined by liquid chromatography (LC) with fluorescence detection (FL). Two chromatographic columns were compared: a high-purity silica Inertsil C(8) column and a newly developed Kinetex C(18) core-shell technology column. Validation was carried out at four concentration levels, using both chromatographic columns. Precision in terms of reproducibility standard deviation was between 7% and 23%, and good recoveries were obtained. Decision limit (CCα) and detection capability (CCβ) values obtained with the Inertsil and Kinetex columns were in the 0.2-19.8 μg kg(-1) and 0.4-33.5 μg kg(-1) concentration ranges, respectively. The proposed method allows residues of quinolones banned for use with laying hens to be detected and quantified in eggs. About twenty-four samples per day can be processed.
authors.affiliation: Departament de Química Analítica, Facultat de Química, Universitat de Barcelona (UB), Martí i Franquès 1-11, 08028 Barcelona, Spain.
authors.forename: V
authors.initials: V
authors.lastname: Jiménez
authors.forename: R
authors.initials: R
authors.lastname: Companyó
authors.forename: J
authors.initials: J
authors.lastname: Guiteras
mesh_terms: D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004531:Eggs / Q000032:analysis*; D005453:Fluorescence; D005504:Food Analysis; D057230:Limit of Detection; D008722:Methods; D015363:Quinolones / Q000032:analysis*; D019155:Veterinary Drugs / Q000032:analysis
chemical_list: D015363:Quinolones; D019155:Veterinary Drugs