title: [The correlation between the up-regulation of Hsp90 and drug resistance to cisplatin in lung cancer cell line].
abstract: OBJECTIVE
  Hsp90 is a major molecular chaperone, which overexpression is involved in oncogenesis, development and drug resistance in many human cancers. The aim of this study is to investigate the relationship between the GGA-induced overexpression of Hsp90 and chemoresistance to Cisplatin in SPCA-1 and H446 cell line.
  
  
  METHODS
  The protein expressions of Hsp90 induced by GGA at different concentrations were analyzed by Immunofluorescence and Western blotting. Cells survival to Cisplatin was determined using the MTT assays. The effect of Hsp90 expression on the drug resistance to Cisplatin in two Cell Lines was analyzed.
  
  
  RESULTS
  Compared with the respective control cells, Hsp90 expressions in both experimental cell lines were up-regulated obviously, exhibiting a dose-dependent manner to GGA. MTT assays revealed that the IC50s of cisplatin also showed a substantial elevation for the experimental cells of SPCA-1 and H446, and this elevation was also associated with GGA concerntration.
  
  
  CONCLUSIONS
  GGA is effective for the induction of Hsp90 in the SPCA-1 and H446 cell line. Up-regulated Hsp90 is associated with the chemoresistance to Cisplatin in SPCA-1and H446 cells.
authors.affiliation: Department of Respiratory Medicine, the Affiliated Zhongshan Hospital of Dalian University, Dalian 116001, China. dlyylt@sina.com
authors.forename: Ting
authors.initials: T
authors.lastname: Liu
authors.forename: Xiaokun
authors.initials: X
authors.lastname: Wang
authors.forename: Lichuan
authors.initials: L
authors.lastname: Zhang
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology*; D004224:Diterpenes / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D019008:Drug Resistance, Neoplasm / Q000187:drug effects*; D018841:HSP90 Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D015854:Up-Regulation / Q000187:drug effects*
chemical_list: D004224:Diterpenes; D018841:HSP90 Heat-Shock Proteins; D002945:Cisplatin; C031049:geranylgeranylacetone