title: NMR resonance assignment of the autoimmunity protein SpaI from Bacillus subtilis ATCC 6633.
abstract: Bacillus subtilis ATCC 6633 produces the lipid II targeting lantibiotic subtilin. For self-protection these gram-positive bacteria express a cluster of four self-immunity proteins named SpaIFEG. SpaI is a 16.8 kDa lipoprotein which is attached to the outside of the cytoplasmic membrane via a covalently linked diacylglycerol anchor. Together with the ABC-transporter SpaFEG, SpaI protects the membrane from subtilin insertion and there is evidence for a direct interaction of SpaI with subtilin. As a prerequisite for further structural studies of SpaI and the SpaI/subtilin complex we report here the full (1)H, (15)N, (13)C chemical shift assignment for a stable 14.9 kDa C-terminal fragment of SpaI.
authors.affiliation: Institut für Molekulare Biowissenschaften, Johann-Wolfgang-Goethe-Universität Frankfurt/M., Max-von-Laue-Str. 9, 60438 Frankfurt, Germany.
authors.forename: Nina Alexandra
authors.initials: NA
authors.lastname: Christ
authors.forename: Elke
authors.initials: E
authors.lastname: Duchardt-Ferner
authors.forename: Stefanie
authors.initials: S
authors.lastname: Düsterhus
authors.forename: Peter
authors.initials: P
authors.lastname: Kötter
authors.forename: Karl-Dieter
authors.initials: KD
authors.lastname: Entian
authors.forename: Jens
authors.initials: J
authors.lastname: Wöhnert
mesh_terms: D015551:Autoimmunity; D001412:Bacillus subtilis; D001426:Bacterial Proteins / Q000737:chemistry* / Q000276:immunology; D008074:Lipoproteins / Q000737:chemistry* / Q000276:immunology; D008565:Membrane Proteins / Q000737:chemistry* / Q000276:immunology; D019906:Nuclear Magnetic Resonance, Biomolecular
chemical_list: D001426:Bacterial Proteins; D008074:Lipoproteins; D008565:Membrane Proteins; C089313:SpaI protein, Bacillus subtilis