title: Detection of high-risk human papillomavirus types 16 and 18 but not 33 and 52 in external genital warts from Iranian females.
abstract: BACKGROUND
  External genital warts (EGW) are relatively common sexually transmitted diseases. In the majority of cases, low-risk human papilomaviruses (HPV), such as HPV-6 and HPV-11, are responsible but, high-risk types may also be detected and this has a bearing on vaccines for cervical cancer prevention. In this study the incidence of the high-risk HPV types 16, 18, 33 and 52 in EGWs of females from the southwest of Iran was assessed.
  
  
  METHODS
  Seventy-nine women with EGWs participated in this study. Quantitative real-time PCR with gene specific primers and probes for the E6 gene of HPV-16, 18, 33 and 52, were used for the detection of HPV DNA in the tissue and blood samples.
  
  
  RESULTS
  Of the 79 tissue specimens, 13 (16.5%) were HPV positive, only genetic materials of HPV-16 and HPV-18 being detected, twelve patients (15.2%) were positive only for HPV-18 and the coexistence of HPV-16 and HPV-18 was shown in one patient. Only one plasma sample showed evidence of HPV-16 with very low viral load.
  
  
  CONCLUSIONS
  Our data showed that high-risk HPV types can be found in the tissue specimens of EGW samples obtained from female patients in the Southwest of Iran, with HPV-18 as the most abundant type; however, additional studies with a larger population are required to prove the finding and help to determine the most appropriate type of virus for vaccine design for Iranian women.
authors.affiliation: Shiraz Institute for Cancer Research, Shiraz University of Medical Sciences, Shiraz, Iran. agahim@sums.ac.ir
authors.forename: Mansooreh
authors.initials: M
authors.lastname: Jaberipour
authors.forename: Mozhdeh
authors.initials: M
authors.lastname: Momtahan
authors.forename: Fatemehsadat
authors.initials: F
authors.lastname: Najib
authors.forename: Sedigheh
authors.initials: S
authors.lastname: Amooei
authors.forename: Farzaneh
authors.initials: F
authors.lastname: Saidifard
authors.forename: Abbas
authors.initials: A
authors.lastname: Ghaderi
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Habibagahi
mesh_terms: D000293:Adolescent; D000328:Adult; D003218:Condylomata Acuminata / Q000188:drug therapy* / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D005838:Genotype; D006801:Humans; D007492:Iran; D008875:Middle Aged; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D055815:Young Adult
chemical_list: D004279:DNA, Viral