title: Characterization of the human serum depletome by label-free shotgun proteomics.
abstract: While it is known that immunoaffinity depletion of abundant proteins in serum removes additional proteins beyond those targeted, there has been little characterization of the co-depleted proteins in the high abundant fraction, which we refer to here as the "depletome". We present evidence of co-depletion of non-targeted proteins in human serum using a top-20 immunodepletion column, as shown by label-free liquid chromatography mass spectrometry (LC-MS(E)) profiling. This led to identification of 147 proteins which were specific for this fraction and comprised proteins with functions predominantly in binding and transport of nucleotides, metal ions, carbohydrates and lipids. These results suggest that further studies on this commonly ignored serum fraction may provide new insights into clinical proteomics.
authors.affiliation: Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, UK.
authors.forename: Theodoros A
authors.initials: TA
authors.lastname: Koutroukides
authors.forename: Paul C
authors.initials: PC
authors.lastname: Guest
authors.forename: F Markus
authors.initials: FM
authors.lastname: Leweke
authors.forename: David M D
authors.initials: DM
authors.lastname: Bailey
authors.forename: Hassan
authors.initials: H
authors.lastname: Rahmoune
authors.forename: Sabine
authors.initials: S
authors.lastname: Bahn
authors.forename: Daniel
authors.initials: D
authors.lastname: Martins-de-Souza
mesh_terms: D001798:Blood Proteins / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D040901:Proteomics / Q000379:methods*; D044967:Serum / Q000737:chemistry*
chemical_list: D001798:Blood Proteins