title: Analysis of intact protein isoforms by mass spectrometry.
abstract: The diverse proteome of an organism arises from such events as single nucleotide substitutions at the DNA level, different RNA processing, and dynamic enzymatic post-translational modifications. This minireview focuses on the measurement of intact proteins to describe the diversity found in proteomes. The field of biological mass spectrometry has steadily advanced, enabling improvements in the characterization of single proteins to proteins derived from cells or tissues. In this minireview, we discuss the basic technology for "top-down" intact protein analysis. Furthermore, examples of studies involved with the qualitative and quantitative analysis of full-length polypeptides are provided.
authors.affiliation: Departmen of Chemistry, Northwestern University, Evanston, Illinois 60208, USA.
authors.forename: Jeremiah D
authors.initials: JD
authors.lastname: Tipton
authors.forename: John C
authors.initials: JC
authors.lastname: Tran
authors.forename: Adam D
authors.initials: AD
authors.lastname: Catherman
authors.forename: Dorothy R
authors.initials: DR
authors.lastname: Ahlf
authors.forename: Kenneth R
authors.initials: KR
authors.lastname: Durbin
authors.forename: Neil L
authors.initials: NL
authors.lastname: Kelleher
mesh_terms: D000818:Animals; D006801:Humans; D048088:Informatics; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D020033:Protein Isoforms / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D020033:Protein Isoforms