title: Impact of exercise training on endothelial transcriptional profiles in healthy swine: a genome-wide microarray analysis.
abstract: While the salutary effects of exercise training on conduit artery endothelial cells have been reported in animals and humans with cardiovascular risk factors or disease, whether a healthy endothelium is alterable with exercise training is less certain. The purpose of this study was to evaluate the impact of exercise training on transcriptional profiles in normal endothelial cells using a genome-wide microarray analysis. Brachial and internal mammary endothelial gene expression was compared between a group of healthy pigs that exercise trained for 16-20 wk (n = 8) and a group that remained sedentary (n = 8). We found that a total of 130 genes were upregulated and 84 genes downregulated in brachial artery endothelial cells with exercise training (>1.5-fold and false discovery rate <15%). In contrast, a total of 113 genes were upregulated and 31 genes downregulated in internal mammary artery endothelial cells using the same criteria. Although there was an overlap of 66 genes (59 upregulated and 7 downregulated with exercise training) between the brachial and internal mammary arteries, the identified endothelial gene networks and biological processes influenced by exercise training were distinctly different between the brachial and internal mammary arteries. These data indicate that a healthy endothelium is indeed responsive to exercise training and support the concept that the influence of physical activity on endothelial gene expression is not homogenously distributed throughout the vasculature.
authors.affiliation: Dept. of Biomedical Sciences, E102 Veterinary Medicine, 1600 E. Rollins Rd., Univ. of Missouri, Columbia, MO 65211, USA. padillaja@missouri.edu
authors.forename: Jaume
authors.initials: J
authors.lastname: Padilla
authors.forename: Grant H
authors.initials: GH
authors.lastname: Simmons
authors.forename: J Wade
authors.initials: JW
authors.lastname: Davis
authors.forename: Jeffrey J
authors.initials: JJ
authors.lastname: Whyte
authors.forename: Theodore W
authors.initials: TW
authors.lastname: Zderic
authors.forename: Marc T
authors.initials: MT
authors.lastname: Hamilton
authors.forename: Douglas K
authors.initials: DK
authors.lastname: Bowles
authors.forename: M Harold
authors.initials: MH
authors.lastname: Laughlin
mesh_terms: D000818:Animals; D001499:Bayes Theorem; D001916:Brachial Artery / Q000166:cytology / Q000378:metabolism*; D042783:Endothelial Cells / Q000378:metabolism*; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks; D016014:Linear Models; D008297:Male; D008323:Mammary Arteries / Q000166:cytology / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D005082:Physical Exertion; D012333:RNA, Messenger / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine; D013556:Swine, Miniature; D013997:Time Factors
chemical_list: D012333:RNA, Messenger