title: Molecular phylogenetic analysis of Theileria species detected from Japanese serows (Capricornis crispus) in Iwate Prefecture, Japan.
abstract: In the present study, we tried to detect DNA for ribosomal RNA genes of piroplasma parasites from the liver or blood of 43 Japanese serows (Capricornis crispus) in Iwate Prefecture of Japan by polymerase chain reaction. Approximately 500-bp amplicons were obtained in 35 (81.4%) of the 43 samples by amplification for V4 hyper-variable regions of the 18S rRNA gene, and the amplicons were considered to be DNA of Theileria species. The complete nucleotide sequences (1,700 or 1709 bp) of the 18S rRNA gene were determined in 20 samples and were divided into 5 genotypes that were phylogenetically separated into two different lineages showing a polyphyletic relation. The Theileria DNAs of the two different lineages were considered to be those of distinct species.
authors.affiliation: Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, Morioka, Japan.
authors.forename: Kazuhito
authors.initials: K
authors.lastname: Ikawa
authors.forename: Mikiko
authors.initials: M
authors.lastname: Aoki
authors.forename: Madoka
authors.initials: M
authors.lastname: Ichikawa
authors.forename: Tadashi
authors.initials: T
authors.lastname: Itagaki
mesh_terms: D000818:Animals; D001483:Base Sequence; D016054:DNA, Protozoan / Q000032:analysis / Q000737:chemistry*; D005838:Genotype; D007564:Japan; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012418:Ruminants / Q000469:parasitology*; D017422:Sequence Analysis, DNA / Q000662:veterinary; D016794:Theileria / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D016054:DNA, Protozoan; D012337:RNA, Ribosomal, 18S