title: The Mg2+ transporter MagT1 partially rescues cell growth and Mg2+ uptake in cells lacking the channel-kinase TRPM7.
abstract: Magnesium (Mg(2+)) transport across membranes plays an essential role in cellular growth and survival. TRPM7 is the unique fusion of a Mg(2+) permeable pore with an active cytosolic kinase domain, and is considered a master regulator of cellular Mg(2+) homeostasis. We previously found that the genetic deletion of TRPM7 in DT40 B cells results in Mg(2+) deficiency and severe growth impairment, which can be rescued by supplementation with excess extracellular Mg(2+). Here, we show that gene expression of the Mg(2+) selective transporter MagT1 is upregulated in TRPM7(-/-) cells. Furthermore, overexpression of MagT1 in TRPM7(-/-) cells augments their capacity to uptake Mg(2+), and improves their growth behavior in the absence of excess Mg(2+).
authors.affiliation: Integrated Department of Immunology, National Jewish Health and University of Colorado, Denver, CO 80206, USA.
authors.forename: Francina
authors.initials: F
authors.lastname: Deason-Towne
authors.forename: Anne-Laure
authors.initials: AL
authors.lastname: Perraud
authors.forename: Carsten
authors.initials: C
authors.lastname: Schmitz
mesh_terms: D000818:Animals; D027682:Cation Transport Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D002645:Chickens; D005786:Gene Expression Regulation; D055785:Gene Knockdown Techniques; D006801:Humans; D008274:Magnesium / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases; D050053:TRPM Cation Channels / Q000235:genetics / Q000378:metabolism*
chemical_list: D027682:Cation Transport Proteins; C509698:MagT1 protein, human; D050053:TRPM Cation Channels; D017346:Protein Serine-Threonine Kinases; C486583:TRPM7 protein, human; D008274:Magnesium