title: Comparison of two commercial ovine Campylobacter vaccines and an experimental bacterin in guinea pigs inoculated with Campylobacter jejuni.
abstract: OBJECTIVE
  To compare efficacy of 2 commercial ovine Campylobacter vaccines and an experimental bacterin in guinea pigs following IP inoculation with Campylobacter jejuni IA3902.
  
  
  METHODS
  51 female guinea pigs.
  
  
  METHODS
  Pregnant and nonpregnant animals were randomly assigned to 1 of 4 treatment groups and administered a commercial Campylobacter vaccine labeled for prevention of campylobacteriosis in sheep via two 5-mL doses 14 days apart (vaccine A; n = 13), another labeled for prevention of campylobacteriosis via two 2-mL doses (vaccine B; 12), an experimental bacterin prepared from the challenge strain (12), or a sham vaccine (14). Ten days later, animals were challenged IP with C jejuni IA3902; 48 hours later, animals were euthanized, complete necropsy was performed, and blood and tissue samples were obtained for bacteriologic culture.
  
  
  RESULTS
  Administration of vaccine B or the experimental bacterin, but not vaccine A, significantly reduced 48-hour infection rates versus administration of the sham vaccine. A significantly reduced 48-hour infection rate was associated with administration of vaccine B independent of pregnancy status.
  
  
  CONCLUSIONS
  Administration of vaccine B significantly reduced infection in guinea pigs challenged with C jejuni IA3902, similar to a homologous bacterin. Results suggested that vaccine B or an autogenous product may be effective in controlling ovine campylobacteriosis caused by this emergent abortifacient strain. Bacteriologic culture of blood, liver, bile, and uterus in nonpregnant guinea pigs 48 hours after inoculation may be a useful screening tool for comparing efficacy of C jejuni vaccines.
authors.affiliation: Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA.
authors.forename: Eric R
authors.initials: ER
authors.lastname: Burrough
authors.forename: Orhan
authors.initials: O
authors.lastname: Sahin
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Plummer
authors.forename: Kevin D
authors.initials: KD
authors.lastname: DiVerde
authors.forename: Qijing
authors.initials: Q
authors.lastname: Zhang
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Yaeger
mesh_terms: D000034:Abortion, Veterinary / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control*; D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology / Q000494:pharmacology*; D002169:Campylobacter Infections / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control / Q000662:veterinary*; D016123:Campylobacter jejuni / Q000145:classification; D021821:Communicable Diseases, Emerging / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control / Q000662:veterinary*; D005260:Female; D006168:Guinea Pigs; D011247:Pregnancy; D012757:Sheep Diseases / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control*; D034561:Sheep, Domestic
chemical_list: D001428:Bacterial Vaccines