title: Allosteric nucleotide-binding site in the mitochondrial NADH:ubiquinone oxidoreductase (respiratory complex I).
abstract: The rotenone-insensitive NADH:hexaammineruthenium III (HAR) oxidoreductase reactions catalyzed by bovine heart and Yarrowia lipolytica submitochondrial particles or purified bovine complex I are stimulated by ATP and other purine nucleotides. The soluble fraction of mammalian complex I (FP) and prokaryotic complex I homolog NDH-1 in Paracoccus denitrificans plasma membrane lack stimulation of their activities by ATP. The stimulation appears as a decrease in apparent K(m) values for NADH and HAR. Thus, the "accessory" subunits of eukaryotic complex I bear an allosteric ATP-binding site.
authors.affiliation: Department of Biochemistry, School of Biology, Moscow State University, Moscow, Russian Federation.
authors.forename: Vera G
authors.initials: VG
authors.lastname: Grivennikova
authors.forename: Grigory V
authors.initials: GV
authors.lastname: Gladyshev
authors.forename: Andrei D
authors.initials: AD
authors.lastname: Vinogradov
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000494:Allosteric Regulation; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D042967:Electron Transport Complex I / Q000737:chemistry* / Q000378:metabolism; D005656:Fungal Proteins / Q000737:chemistry* / Q000378:metabolism; D008928:Mitochondria / Q000201:enzymology*; D009243:NAD / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000737:chemistry* / Q000378:metabolism; D009711:Nucleotides / Q000378:metabolism*; D010231:Paracoccus denitrificans / Q000166:cytology / Q000378:metabolism; D017975:Ruthenium Compounds / Q000378:metabolism; D025062:Yarrowia / Q000166:cytology / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D009711:Nucleotides; D017975:Ruthenium Compounds; D009243:NAD; C031584:hexammineruthenium; D000255:Adenosine Triphosphate; D009247:NADH, NADPH Oxidoreductases; D042967:Electron Transport Complex I