title: Triterpenes involved in the anti-inflammatory effect of ethanolic extract of Pterodon emarginatus Vogel stem bark.
abstract: Bioassay-guided fractionation of the ethanolic extract of Pterodon emarginatus Vogel stem bark (EtEx) resulted in the isolation and characterization of lupeol and betulin. Their structures were elucidated by spectroscopic methods including IR, (1)H-NMR, (13)C-NMR and comparison with literature values. This study showed the anti-inflammatory activity of EtEx, the hexane (HexL) and dichloromethane (DichL) layers, and lupeol and betulin. The extract, HexL, DichL, lupeol and betulin were able to inhibit acetic acid-induced writhing. In the formalin test, EtEx decreased licking time only in the second phase characterizing anti-inflammatory activity. In the oil-induced ear oedema test, EtEx, lupeol and betulin decrease edema formation. In conclusion, EtEx has antinociceptive effects arising from anti-inflammatory activity; this activity could be due to the presence of lupeol and betulin.
authors.affiliation: Laboratório de Pesquisa em Produtos Naturais, Faculdade de Farmácia, Universidade Federal de Goiás, Goiânia, GO, Brazil.
authors.forename: Weuller F
authors.initials: WF
authors.lastname: de Moraes
authors.forename: Pablinny Moreira
authors.initials: PM
authors.lastname: Galdino
authors.forename: Marcus Vinícius M
authors.initials: MV
authors.lastname: Nascimento
authors.forename: Frederico Argollo
authors.initials: FA
authors.lastname: Vanderlinde
authors.forename: Maria Teresa F
authors.initials: MT
authors.lastname: Bara
authors.forename: Elson A
authors.initials: EA
authors.lastname: Costa
authors.forename: José Realino
authors.initials: JR
authors.lastname: de Paula
mesh_terms: D019342:Acetic Acid; D000700:Analgesics / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D005591:Chemical Fractionation; D003436:Croton Oil; D004195:Disease Models, Animal; D000431:Ethanol / Q000737:chemistry*; D007887:Fabaceae / Q000737:chemistry; D005557:Formaldehyde; D006586:Hexanes / Q000737:chemistry; D007249:Inflammation / Q000139:chemically induced / Q000517:prevention & control*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008752:Methylene Chloride / Q000737:chemistry; D051379:Mice; D015394:Molecular Structure; D010146:Pain / Q000139:chemically induced / Q000517:prevention & control; D053978:Pentacyclic Triterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D024301:Plant Bark; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018547:Plant Stems; D012997:Solvents / Q000737:chemistry*; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000094062:Betulinic Acid; D000094042:Lupanes
chemical_list: D019342:Acetic Acid; D000700:Analgesics; D000893:Anti-Inflammatory Agents; D003436:Croton Oil; D000431:Ethanol; D005557:Formaldehyde; D006586:Hexanes; D008752:Methylene Chloride; D053978:Pentacyclic Triterpenes; D010936:Plant Extracts; D012997:Solvents; D014315:Triterpenes; C002503:betulin; D000094062:Betulinic Acid; C010480:lupeol; D000094042:Lupanes