title: Investigation of the effective action distance between hematopoietic stem/progenitor cells and human adipose-derived stem cells during their in vitro co-culture.
abstract: The in vitro suitable action distance between umbilical cord blood-derived hematopoietic stem/progenitor cells and its feeder cell, human adipose-derived stem cells, during their co-culture, was investigated through a novel transwell co-culture protocol, in which the distance between the two culture chambers where each cell type is growing can be adjusted from 10 to 450 μm. The total cell number was determined with a hemacytometer, and the cell morphology was observed under an inverted microscope each day. After 7 days of co-culture, the fold-expansion, surface antigen expression of CD34(+) and CFU-GM assay of the hematopoietic mononuclear cells (MNCs) were analyzed. The results showed that there was an optimal communication distance at around 350 μm between both types of stem cells during their in vitro co-culture. By using this distance, the UCB-MNCs and CD34(+) cells were expanded by 15.1 ± 0.2 and 5.0 ± 0.1-fold, respectively. It can therefore be concluded that the optimal action distance between stem cells and their supportive cells, when cultured together for 7 days, is of around 350 μm.
authors.affiliation: Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian 116024, China.
authors.forename: Kedong
authors.initials: K
authors.lastname: Song
authors.forename: Hai
authors.initials: H
authors.lastname: Wang
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.forename: Mo
authors.initials: M
authors.lastname: Qiao
authors.forename: Shuang
authors.initials: S
authors.lastname: Wu
authors.forename: Tianqing
authors.initials: T
authors.lastname: Liu
mesh_terms: D018952:Antigens, CD34 / Q000032:analysis; D002450:Cell Communication; D049109:Cell Proliferation; D018920:Coculture Techniques / Q000379:methods; D015194:Diffusion Chambers, Culture; D061252:Feeder Cells; D005260:Female; D005312:Fetal Blood / Q000166:cytology / Q000378:metabolism*; D055014:Granulocyte-Macrophage Progenitor Cells / Q000166:cytology / Q000378:metabolism*; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D011247:Pregnancy
chemical_list: D018952:Antigens, CD34