title: Identification of volatiles from pineapple (Ananas comosus L.) pulp by comprehensive two-dimensional gas chromatography and gas chromatography/mass spectrometry.
abstract: Combining qualitative data from the chromatographic structure of 2-D gas chromatography with flame ionization detection (GC×GC-FID) and that from gas chromatography-mass spectrometry (GC/MS) should result in a more accurate assignment of the peak identities than the simple analysis by GC/MS, where coelution of analytes is unavoidable in highly complex samples (rendering spectra unsuitable for qualitative purposes) or for compounds in very low concentrations. Using data from GC×GC-FID combined with GC/MS can reveal coelutions that were not detected by mass spectra deconvolution software. In addition, some compounds can be identified according to the structure of the GC×GC-FID chromatogram. In this article, the volatile fractions of fresh and dehydrated pineapple pulp were evaluated. The extraction of the volatiles was performed by dynamic headspace extraction coupled to solid-phase microextraction (DHS-SPME), a technique appropriate for slurries or solid matrices. Extracted analytes were then analyzed by GC×GC-FID and GC/MS. The results obtained using both techniques were combined to improve compound identifications.
authors.affiliation: Institute of Chemistry, State University of Campinas (Unicamp) and Instituto Nacional de Ciência e Tecnologia de Bioanalítica (INCTBio), São Paulo, Brazil. marciopedroso@dqi.ufla.br
authors.forename: Marcio P
authors.initials: MP
authors.lastname: Pedroso
authors.forename: Ernesto C
authors.initials: EC
authors.lastname: Ferreira
authors.forename: Leandro W
authors.initials: LW
authors.lastname: Hantao
authors.forename: Stanislau
authors.initials: S
authors.lastname: Bogusz
authors.forename: Fabio
authors.initials: F
authors.lastname: Augusto
mesh_terms: D031230:Ananas / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D010936:Plant Extracts / Q000032:analysis* / Q000302:isolation & purification; D052617:Solid Phase Microextraction; D055549:Volatile Organic Compounds / Q000032:analysis* / Q000302:isolation & purification
chemical_list: D010936:Plant Extracts; D055549:Volatile Organic Compounds