title: The mechanism of substrate recognition of Ca2+-dependent protein kinases.
abstract: Ca2+-dependent protein kinases (CDPKs) are encoded by a multigene family and are thought to play central roles in Ca2+ signaling in plants. Although the primary structures of CDPK isoforms are highly conserved, several studies suggested a distinct physiological function for each CDPK isoform in plants. Hence, there should be mechanisms by which individual CDPK specifically recognizes its substrate. Recently, the variable N-terminal domain of NtCDPK1 was shown to play an essential role in the specific recognition of the substrate. Because the variable N-terminal domain of other CDPKs may also be involved in the substrate recognition, the search for interacting proteins of the variable N-terminal domain would provide important clues to identify the physiological substrates of each CDPK. Additionally, manipulation of the variable N-terminal domain may enable us to engineer the substrate specificity of CDPK, leading a rational rewiring of cellular signaling pathways.
authors.affiliation: Department of Biological Science, Graduate School of Science, Hiroshima University, Kagamiyama, Higashi-Hiroshima, Japan. itotakes@hiroshima-u.ac.jp
authors.forename: Takeshi
authors.initials: T
authors.lastname: Ito
authors.forename: Masaru
authors.initials: M
authors.lastname: Nakata
authors.forename: Sarahmi
authors.initials: S
authors.lastname: Ishida
authors.forename: Yohsuke
authors.initials: Y
authors.lastname: Takahashi
mesh_terms: D002118:Calcium / Q000378:metabolism*; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D011494:Protein Kinases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology; D013379:Substrate Specificity
chemical_list: D010940:Plant Proteins; D011494:Protein Kinases; D002118:Calcium