title: A new route to dTDP-6-deoxy-l-talose and dTDP-L-rhamnose: dTDP-L-rhamnose 4-epimerase in Burkholderia thailandensis.
abstract: dTDP-L-rhamnose (dTDP-Rha)-synthesizing dTDP-6-deoxy-L-lyxo-4-hexulose reductase (4-KR) and dTDP-Rha 4-epimerase were characterized from Burkholderia thailandensis E264 by utilizing rmlD(Bth) (BTH_I1472) and wbiB(Bth) (BTH_I1476), respectively. Incubation of the recombinant WbiB(Bth) with RmlA/RmlB/RmlC/Tal, which has previously been shown to generate dTDP-6-deoxy-L-talose (dTDP-6dTal) from α-D-glucose-1-phosphate, dTTP, and NADPH, produced dTDP-Rha. (1)H NMR measurements confirmed that both RmlA/RmlB/RmlC/Tal/WbiB(Bth) and RmlA/RmlB/RmlC/RmlD produced dTDP-Rha. WbiB(Bth) alone produced dTDP-Rha when incubated with dTDP-6dTal. This is the first report to demonstrate epimerase activity interconverting between dTDP-Rha and dTDP-6dTal.
authors.affiliation: Department of Biological Science, Division of Bioscience and Bioinformatics, Myongji University, Yongin 449-728, Republic of Korea.
authors.forename: Hye-Gyeong
authors.initials: HG
authors.lastname: Yoo
authors.forename: So-Yeon
authors.initials: SY
authors.lastname: Kwon
authors.forename: Suman
authors.initials: S
authors.lastname: Karki
authors.forename: Hyung-Jin
authors.initials: HJ
authors.lastname: Kwon
mesh_terms: D019117:Burkholderia / Q000201:enzymology* / Q000235:genetics; D002238:Carbohydrate Epimerases / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D003837:Deoxy Sugars / Q000737:chemistry*; D006601:Hexoses / Q000737:chemistry*; D015394:Molecular Structure; D009702:Nucleoside Diphosphate Sugars / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D013942:Thymine Nucleotides / Q000737:chemistry*
chemical_list: D003837:Deoxy Sugars; D006601:Hexoses; D009702:Nucleoside Diphosphate Sugars; D011994:Recombinant Proteins; D013942:Thymine Nucleotides; C073293:thymidine diphosphate rhamnose; C011754:6-deoxytalose; D002238:Carbohydrate Epimerases