title: Comparison of genotypic and phylogenetic relationships of environmental Enterococcus isolates by BOX-PCR typing and 16S rRNA gene sequencing.
abstract: Environmental Enterococcus spp. were compared by BOX-PCR genotyping and 16S rRNA gene sequencing to clarify the predictive relationship of BOX-PCR fingerprints to species designation. BOX-PCR and 16S rRNA gene relationships agreed for 77% of strains. BOX-PCR provided superior intraspecies discrimination but incorrectly identified some strains to the species level and divided some species into multiple groups.
authors.affiliation: Department of Integrative Biology, University of South Florida, Tampa, FL 33620, USA.
authors.forename: Bina S
authors.initials: BS
authors.lastname: Nayak
authors.forename: Brian
authors.initials: B
authors.lastname: Badgley
authors.forename: Valerie J
authors.initials: VJ
authors.lastname: Harwood
mesh_terms: D001483:Base Sequence; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D016983:Enterococcus / Q000235:genetics* / Q000302:isolation & purification*; D005838:Genotype; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D012335:RNA, Ribosomal / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012335:RNA, Ribosomal; D012336:RNA, Ribosomal, 16S