title: Isolation and characterization of Thermanaerothrix daxensis gen. nov., sp. nov., a thermophilic anaerobic bacterium pertaining to the phylum "Chloroflexi", isolated from a deep hot aquifer in the Aquitaine Basin.
abstract: A new strictly anaerobic thermophilic multicellular filamentous bacterium (0.2-0.3μm×>100μm), designated GNS-1(T), was isolated from a deep hot aquifer in France. It was non-motile, and stained Gram-negative. Optimal growth was observed at 65°C, pH 7.0, and 2gL(-1) of NaCl. Strain GNS-1(T) was chemoorganotrophic fermenting ribose, glucose, galactose, arabinose, fructose, mannose, maltose, sucrose, xylose, raffinose, pyruvate, and xylan. Yeast extract was required for growth. The end products of glucose fermentation were lactate, acetate, CO(2), and H(2). The G+C content of the DNA was 57.6mol%. Its closest phylogenetic relative was Bellilinea caldifistulae with 92.5% similarity. Based on phylogenetic, genotypic and phenotypic characteristics, strain GNS-1(T) (DSM 23592(T), JCM 16980(T)) is proposed to be assigned to a novel species of a novel genus within the class Anaerolineae (subphylum I), phylum "Chloroflexi", Thermanaerothrix daxensis gen. nov., sp. nov. The GenBank accession number is HM596746.
authors.affiliation: Laboratoire de Microbiologie et de Biotechnologie des Environnements Chauds UMR_D 180, IRD, Universités de Provence et de la Méditerranée, 13288 Marseille cedex 9, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Grégoire
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Fardeau
authors.forename: Manon
authors.initials: M
authors.lastname: Joseph
authors.forename: Sophie
authors.initials: S
authors.lastname: Guasco
authors.forename: Francette
authors.initials: F
authors.lastname: Hamaide
authors.forename: Sandra
authors.initials: S
authors.lastname: Biasutti
authors.forename: Valérie
authors.initials: V
authors.lastname: Michotey
authors.forename: Patricia
authors.initials: P
authors.lastname: Bonin
authors.forename: Bernard
authors.initials: B
authors.lastname: Ollivier
mesh_terms: D000085:Acetates / Q000378:metabolism; D015373:Bacterial Typing Techniques; D001482:Base Composition; D001483:Base Sequence; D050260:Carbohydrate Metabolism; D002473:Cell Wall / Q000378:metabolism; D041862:Chloroflexi / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D005285:Fermentation; D005602:France; D005798:Genes, Bacterial; D020459:Genes, rRNA; D005947:Glucose / Q000378:metabolism; D006089:Gram-Negative Anaerobic Bacteria / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D045482:Hot Springs / Q000382:microbiology*; D006358:Hot Temperature; D006859:Hydrogen / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid / Q000378:metabolism; D008858:Microscopy, Phase-Contrast; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014871:Water Microbiology
chemical_list: D000085:Acetates; D012336:RNA, Ribosomal, 16S; D019344:Lactic Acid; D006859:Hydrogen; D005947:Glucose