title: Trapping conformational states along ligand-binding dynamics of peptide deformylase: the impact of induced fit on enzyme catalysis.
abstract: For several decades, molecular recognition has been considered one of the most fundamental processes in biochemistry. For enzymes, substrate binding is often coupled to conformational changes that alter the local environment of the active site to align the reactive groups for efficient catalysis and to reach the transition state. Adaptive substrate recognition is a well-known concept; however, it has been poorly characterized at a structural level because of its dynamic nature. Here, we provide a detailed mechanism for an induced-fit process at atomic resolution. We take advantage of a slow, tight binding inhibitor-enzyme system, actinonin-peptide deformylase. Crystal structures of the initial open state and final closed state were solved, as well as those of several intermediate mimics captured during the process. Ligand-induced reshaping of a hydrophobic pocket drives closure of the active site, which is finally "zipped up" by additional binding interactions. Together with biochemical analyses, these data allow a coherent reconstruction of the sequence of events leading from the encounter complex to the key-lock binding state of the enzyme. A "movie" that reconstructs this entire process can be further extrapolated to catalysis.
authors.affiliation: CNRS, ISV, UPR2355, Gif-sur-Yvette, France.
authors.forename: Sonia
authors.initials: S
authors.lastname: Fieulaine
authors.forename: Adrien
authors.initials: A
authors.lastname: Boularot
authors.forename: Isabelle
authors.initials: I
authors.lastname: Artaud
authors.forename: Michel
authors.initials: M
authors.lastname: Desmadril
authors.forename: Frédéric
authors.initials: F
authors.lastname: Dardel
authors.forename: Thierry
authors.initials: T
authors.lastname: Meinnel
authors.forename: Carmela
authors.initials: C
authors.lastname: Giglione
mesh_terms: D000581:Amidohydrolases / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000235:genetics; D020816:Amino Acid Motifs; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000235:genetics; D020134:Catalytic Domain; D018360:Crystallography, X-Ray; D004791:Enzyme Inhibitors / Q000737:chemistry*; D006860:Hydrogen Bonding; D006877:Hydroxamic Acids / Q000737:chemistry; D007700:Kinetics; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D020125:Mutation, Missense; D011485:Protein Binding / Q000235:genetics; D013816:Thermodynamics
chemical_list: D029681:Arabidopsis Proteins; D004791:Enzyme Inhibitors; D006877:Hydroxamic Acids; D000581:Amidohydrolases; C561287:peptide deformylase 1B, Arabidopsis; C010531:actinonin