title: Suppression of beta-naphthoflavone induced CYP1A expression and lipid-peroxidation by berberine.
abstract: Impacts of berberine, a major isoquinoline alkaloid in herbal plants, on beta-naphthoflavone (BNF)-induced CYP1A expression were determined both in primary mouse hepatocytes and in vivo. Berberine concentration-dependently suppressed BNF-induced CYP1A expression in mouse hepatocyte and it significantly down-regulated BNF-induced CYP1A in mouse liver via suppression of mRNA and protein expression, and decreases of EROD and MROD activities. Moreover, berberine showed significant potential on suppression of BNF-induced lipid peroxidation in mouse hepatic microsome. Therefore, using berberine as a health supplement or an alternative medication might provide extra-benefit due to its inhibitory regulation on CYP1A expression and anti-lipid peroxidation activity.
authors.affiliation: Academic Office for Pharmaceutical Sciences, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002 Thailand.
authors.forename: Waranya
authors.initials: W
authors.lastname: Chatuphonprasert
authors.forename: Thewtas
authors.initials: T
authors.lastname: Sangkawat
authors.forename: Nobuo
authors.initials: N
authors.lastname: Nemoto
authors.forename: Kanokwan
authors.initials: K
authors.lastname: Jarukamjorn
mesh_terms: D000818:Animals; D001599:Berberine / Q000737:chemistry / Q000494:pharmacology*; D019363:Cytochrome P-450 CYP1A1 / Q000187:drug effects* / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D022781:Hepatocytes / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010946:Plants, Medicinal / Q000737:chemistry; D012333:RNA, Messenger / Q000235:genetics; D019324:beta-Naphthoflavone / Q000009:adverse effects*
chemical_list: D012333:RNA, Messenger; D001599:Berberine; D019324:beta-Naphthoflavone; D019363:Cytochrome P-450 CYP1A1