title: Presence of galactosylated core fucose on N-glycans in the planaria Dugesia japonica.
abstract: Planarial species are of especial interest to biologists due to the phenomenon of pluripotency and, in comparison to other developmental processes, it can be hypothesised that glycan-lectin interactions may play a role. In order to examine the N-glycans of one of these organisms, Dugesia japonica, peptide:N-glycosidase A was employed and the released glycans were subject to pyridylamination, HPLC and mass spectrometric analysis. A range of oligomannosidic glycans was observed with a trimethylated Man(5) GlcNAc(2) structure being the dominant species. Three glycans were also observed to contain deoxyhexose; in particular, a glycan with the composition Hex(4) HexNAc(2) Fuc(1) Me(2) was revealed by exoglycosidase digestion, in combination with MS/MS, to contain a galactosylated core α1,6-fucose residue, whereas this core modification was found to be capped with a methylhexose residue in the case of a Hex(5) HexNAc(2) Fuc(1) Me(3) structure. This is the first report of these types of structures in a platyhelminth and indicates that the 'GalFuc' modification of N-glycans is not just restricted to molluscs and nematodes.
authors.affiliation: Department für Chemie, Universität für Bodenkultur, Muthgasse, Wien, Austria.
authors.forename: Katharina
authors.initials: K
authors.lastname: Paschinger
authors.forename: Ebrahim
authors.initials: E
authors.lastname: Razzazi-Fazeli
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Furukawa
authors.forename: Iain B H
authors.initials: IB
authors.lastname: Wilson
mesh_terms: D000818:Animals; D002236:Carbohydrate Conformation; D002851:Chromatography, High Pressure Liquid; D005643:Fucose / Q000737:chemistry* / Q000378:metabolism; D005690:Galactose / Q000737:chemistry* / Q000378:metabolism; D008745:Methylation; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase / Q000737:chemistry / Q000378:metabolism; D010932:Planarians / Q000737:chemistry* / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry* / Q000378:metabolism; D053719:Tandem Mass Spectrometry
chemical_list: D009844:Oligosaccharides; D011134:Polysaccharides; D005643:Fucose; C058642:mannosyl(5)-N-acetyl(2)-glucose; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase; D005690:Galactose