title: Crystallization and preliminary crystallographic studies of Helicobacter pylori arginase.
abstract: Helicobacter pylori arginase is an important factor in evasion of the host's immune system and contributes to persistent infection by this bacterium. It is unique in many aspects compared with other arginases: for example, it has optimal activity with Co(2+) as a cofactor rather than Mn(2+) and has strongest activity at acidic pH instead of alkaline pH. In this study, H. pylori arginase was purified and crystallized in complex with Mn(2+) and a diffraction data set was collected to 2.2 Å resolution. The crystals belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 94.69, b = 102.24, c = 148.61 Å.
authors.affiliation: Department of Clinical Microbiology and Immunology, College of Medical Laboratory Science, Third Military Medical University, Chongqing, People's Republic of China.
authors.forename: Jinyong
authors.initials: J
authors.lastname: Zhang
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Zhang
authors.forename: Xuhu
authors.initials: X
authors.lastname: Mao
authors.forename: Quanming
authors.initials: Q
authors.lastname: Zou
authors.forename: Defeng
authors.initials: D
authors.lastname: Li
mesh_terms: D001119:Arginase / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D016480:Helicobacter pylori / Q000201:enzymology*
chemical_list: D001119:Arginase