title: Plasma proteome changes in cardiovascular disease patients: novel isoforms of apolipoprotein A1.
abstract: BACKGROUND
  The aim of this proteomic study was to look for changes taking place in plasma proteomes of patients with acute myocardial infarction (AMI), unstable angina pectoris (UAP), and stable angina pectoris (SAP).
  
  
  METHODS
  Depleted plasma proteins were separated by 2D SDS-PAGE (pI 4-7), and proteomes were compared using Progenesis SameSpots statistical software. Proteins were identified by nanoLC-MS/MS. Proteins were quantified using commercial kits. Apolipoprotein A1 was studied using 1D and 2D SDS-PAGE, together with western blotting.
  
  
  RESULTS
  Reciprocal comparison revealed 46 unique, significantly different spots; proteins in 34 spots were successfully identified and corresponded to 38 different proteins. Discrete comparisons of patient groups showed 45, 41, and 8 significantly different spots when AMI, UAP, and SAP were compared with the control group. On the basis of our proteomic data, plasma levels of two of them, alpha-1 microglobulin and vitamin D-binding protein, were determined. The data, however, failed to prove the proteins to be suitable markers or risk factors in the studied groups. The plasma level and isoform representation of apolipoprotein A1 were also estimated. Using 1D and 2D SDS-PAGE, together with western blotting, we observed extra high-molecular weight apolipoprotein A1 fractions presented only in the patient groups, indicating that the novel high-molecular weight isoforms of apolipoprotein A1 may be potential new markers or possible risk factors of cardiovascular disease.
  
  
  CONCLUSIONS
  The reported data show plasma proteome changes in patients with AMI, UAP, and SAP. We propose some apolipoprotein A1 fractions as a possible new disease-associated marker of cardiovascular disorders.
authors.affiliation: Institute of Hematology and Blood Transfusion, Prague, Czech Republic. pavel.majek@uhkt.cz
authors.forename: Pavel
authors.initials: P
authors.lastname: Májek
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Reicheltová
authors.forename: Jiří
authors.initials: J
authors.lastname: Suttnar
authors.forename: Martin
authors.initials: M
authors.lastname: Malý
authors.forename: Milan
authors.initials: M
authors.lastname: Oravec
authors.forename: Klára
authors.initials: K
authors.lastname: Pečánková
authors.forename: Jan E
authors.initials: JE
authors.lastname: Dyr
mesh_terms: D000368:Aged; D016632:Apolipoprotein A-I / Q000097:blood*; D002318:Cardiovascular Diseases / Q000097:blood*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D008297:Male; D008970:Molecular Weight; D025341:Principal Component Analysis; D020033:Protein Isoforms / Q000097:blood; D020543:Proteome / Q000378:metabolism*
chemical_list: D016632:Apolipoprotein A-I; D020033:Protein Isoforms; D020543:Proteome