title: Full sequence analysis of the original Sapporo virus.
abstract: In this study, the full-length genome sequence of the prototype of sapovirus, namely Sapporo virus (SV82), was identified. Sapporo virus RNA was extracted from a fecal sample, amplified by RT-PCR and the PCR products sequenced directly and analyzed. Sequence analysis showed that Sapporo virus consists of 7433 nucleotides and has three open reading frames. The Sapporo strain shows 91.7% nucleotide sequence identity to the Manchester virus. Phylogenic analysis has also revealed the closeness of Sapporo virus to other sapovirus/genogroup I strains. Basic information on the evolutionary history of sapovirus analysis is provided here.
authors.affiliation: Department of Pediatrics, Sapporo Medical University School of Medicine, South-1, West-16, Chuo-Ku, Sapporo 060-8543, Japan.
authors.forename: Kaori
authors.initials: K
authors.lastname: Nakanishi
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Tatsumi
authors.forename: Kazuko
authors.initials: K
authors.lastname: Kinoshita-Numata
authors.forename: Takeshi
authors.initials: T
authors.lastname: Tsugawa
authors.forename: Shuji
authors.initials: S
authors.lastname: Nakata
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tsutsumi
mesh_terms: D016000:Cluster Analysis; D005243:Feces / Q000821:virology; D016679:Genome, Viral; D006801:Humans; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D029341:Sapovirus / Q000235:genetics* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D012367:RNA, Viral