title: Genetic diversity in cultivated carioca common beans based on molecular marker analysis.
abstract: A wide array of molecular markers has been used to investigate the genetic diversity among common bean species. However, the best combination of markers for studying such diversity among common bean cultivars has yet to be determined. Few reports have examined the genetic diversity of the carioca bean, commercially one of the most important common beans in Brazil. In this study, we examined the usefulness of two molecular marker systems (simple sequence repeats - SSRs and amplified fragment length polymorphisms - AFLPs) for assessing the genetic diversity of carioca beans. The amount of information provided by Roger's modified genetic distance was used to analyze SSR data and Jaccards similarity coefficient was used for AFLP data. Seventy SSRs were polymorphic and 20 AFLP primer combinations produced 635 polymorphic bands. Molecular analysis showed that carioca genotypes were quite diverse. AFLPs revealed greater genetic differentiation and variation within the carioca genotypes (Gst = 98% and Fst = 0.83, respectively) than SSRs and provided better resolution for clustering the carioca genotypes. SSRs and AFLPs were both suitable for assessing the genetic diversity of Brazilian carioca genotypes since the number of markers used in each system provided a low coefficient of variation. However, fingerprint profiles were generated faster with AFLPs, making them a better choice for assessing genetic diversity in the carioca germplasm.
authors.affiliation: Centro de Recursos Genéticos Vegetais, Instituto Agronômico de Campinas, Campinas, SP, Brazil.
authors.forename: Juliana Morini
authors.initials: JM
authors.lastname: Küpper Cardoso Perseguini
authors.forename: Alisson Fernando
authors.initials: AF
authors.lastname: Chioratto
authors.forename: Maria Imaculada
authors.initials: MI
authors.lastname: Zucchi
authors.forename: Carlos Augusto
authors.initials: CA
authors.lastname: Colombo
authors.forename: Sérgio Augusto Moraes
authors.initials: SA
authors.lastname: Carbonell
authors.forename: Jorge Mauricio
authors.initials: JM
authors.lastname: Costa Mondego
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Gazaffi
authors.forename: Antonio Augusto
authors.initials: AA
authors.lastname: Franco Garcia
authors.forename: Tatiana
authors.initials: T
authors.lastname: de Campos
authors.forename: Anete Pereira
authors.initials: AP
authors.lastname: de Souza
authors.forename: Luciana Benchimol
authors.initials: LB
authors.lastname: Rubiano