title: Targeted mRNA oxidation regulates sunflower seed dormancy alleviation during dry after-ripening.
abstract: After-ripening is the mechanism by which dormant seeds become nondormant during their dry storage after harvest. The absence of free water in mature seeds does not allow detectable metabolism; thus, the processes associated with dormancy release under these conditions are largely unknown. We show here that sunflower (Helianthus annuus) seed alleviation of dormancy during after-ripening is associated with mRNA oxidation and that this oxidation is prevented when seeds are maintained dormant. In vitro approaches demonstrate that mRNA oxidation results in artifacts in cDNA-amplified fragment length polymorphim analysis and alters protein translation. The oxidation of transcripts is not random but selective, and, using microarrays, we identified 24 stored mRNAs that became highly oxidized during after-ripening. Oxidized transcripts mainly correspond to genes involved in responses to stress and in cell signaling. Among them, protein phosphatase 2C PPH1, mitogen-activated protein kinase phosphatase 1, and phenyl ammonia lyase 1 were identified. We propose that targeted mRNA oxidation during dry after-ripening of dormant seeds could be a process that governs cell signaling toward germination in the early steps of seed imbibition.
authors.affiliation: UR5 EAC7180 Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, Université Paris 06, 75005 Paris, France.
authors.forename: Jérémie
authors.initials: J
authors.lastname: Bazin
authors.forename: Nicolas
authors.initials: N
authors.lastname: Langlade
authors.forename: Patrick
authors.initials: P
authors.lastname: Vincourt
authors.forename: Sandrine
authors.initials: S
authors.lastname: Arribat
authors.forename: Sandrine
authors.initials: S
authors.lastname: Balzergue
authors.forename: Hayat
authors.initials: H
authors.lastname: El-Maarouf-Bouteau
authors.forename: Christophe
authors.initials: C
authors.lastname: Bailly
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D020869:Gene Expression Profiling; D018525:Germination / Q000502:physiology; D006368:Helianthus / Q000033:anatomy & histology / Q000502:physiology*; D046228:Microarray Analysis; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D057445:Plant Dormancy / Q000502:physiology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism*; D012639:Seeds / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology; D059467:Transcriptome
chemical_list: D010940:Plant Proteins; D012333:RNA, Messenger; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine