title: Comparison of mRNA expression patterns of class B scavenger receptors in BV2 microglia upon exposure to amyloidogenic fragments of beta-amyloid and prion proteins.
abstract: The inflammatory responses in Alzheimer's disease and prion diseases are dominated by microglia activation. Scavenger receptors have been recently related to the innate immune activation of microglia initiated by endogenous ligands. In this study, we investigated mRNA expression patterns of B class scavenger receptors CD36 and scavenger receptor B1 (SR-B1) in BV2 microglia upon exposure to amyloid fibril Aβ(1-42) and PrP(106-126), respectively. CD36 and SR-B1 showed similar mRNA expression patterns following each treatment. PrP(106-126) induced a rapid increase of CD36 and SR-B1 mRNA levels in the treated microglia, whereas Aβ(1-42) induced a delayed but persistent increase in the mRNA expression of CD36 and SR-B1. These results suggest a possible involvement of CD36 and SR-B1 in microglial interaction with amyloidogenic fragments of beta-amyloid and prion proteins.
authors.affiliation: State Key Laboratories for Agrobiotechnology, College of Veterinary Medicine, China Agricultural University, Beijing, China.
authors.forename: Mohammed
authors.initials: M
authors.lastname: Kouadir
authors.forename: Lifeng
authors.initials: L
authors.lastname: Yang
authors.forename: Jian
authors.initials: J
authors.lastname: Tu
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Yin
authors.forename: Xiangmei
authors.initials: X
authors.lastname: Zhou
authors.forename: Deming
authors.initials: D
authors.lastname: Zhao
mesh_terms: D000595:Amino Acid Sequence; D016229:Amyloid beta-Peptides / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D018955:CD36 Antigens / Q000235:genetics*; D002460:Cell Line; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D051379:Mice; D017628:Microglia / Q000187:drug effects* / Q000378:metabolism*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000494:pharmacology*; D011328:Prions / Q000737:chemistry / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D013997:Time Factors
chemical_list: D016229:Amyloid beta-Peptides; D018955:CD36 Antigens; D010446:Peptide Fragments; D011328:Prions; D012333:RNA, Messenger; C082632:prion protein (106-126)