title: The Botrytis cinerea Reg1 protein, a putative transcriptional regulator, is required for pathogenicity, conidiogenesis, and the production of secondary metabolites.
abstract: Botrytis cinerea, which causes gray-mold rot, attacks a wide range of plant species. To understand the infection process, the role of a putative transcriptional regulator, BcReg1 (regulator 1), in pathogenicity was studied. This transcriptional regulator shows similarity to the morphological switch regulators Candida albicans Wor1 and Histoplasma capsulatum Ryp1. Gene knock-out and complementation studies revealed that bcreg1 is required for pathogenicity. The bcreg1 mutant is able to penetrate plant tissue but is not able to cause necrotic lesions. In addition, the mutant is blocked in conidia formation and does not produce detectable levels of the sesquiterpene botrydial and the polyketide botcinic acid. Based on transcript expression levels, it can be concluded that bcreg1 is a downstream target of two mitogen-activated protein kinases, BcSak1 and Bmp3.
authors.affiliation: Westfalische Wilhelms-University, Munster, Germany. C.B.Michielse@uni-muenster.de
authors.forename: Caroline B
authors.initials: CB
authors.lastname: Michielse
authors.forename: Matthias
authors.initials: M
authors.lastname: Becker
authors.forename: Jens
authors.initials: J
authors.lastname: Heller
authors.forename: Javier
authors.initials: J
authors.lastname: Moraga
authors.forename: Isidro G
authors.initials: IG
authors.lastname: Collado
authors.forename: Paul
authors.initials: P
authors.lastname: Tudzynski
mesh_terms: D000595:Amino Acid Sequence; D020171:Botrytis / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity* / Q000648:ultrastructure; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D005816:Genetic Complementation Test; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009183:Mycotoxins / Q000096:biosynthesis / Q000235:genetics; D018384:Oxidative Stress; D027805:Phaseolus / Q000382:microbiology; D010935:Plant Diseases / Q000382:microbiology; D018515:Plant Leaves; D012331:RNA, Fungal / Q000235:genetics; D016415:Sequence Alignment; D017384:Sequence Deletion; D015398:Signal Transduction; D013172:Spores, Fungal / Q000254:growth & development*; D013312:Stress, Physiological / Q000235:genetics; D014774:Virulence / Q000235:genetics
chemical_list: D004271:DNA, Fungal; D005656:Fungal Proteins; D009183:Mycotoxins; D012331:RNA, Fungal; D020928:Mitogen-Activated Protein Kinases