title: In smokers, swim-up and discontinuous gradient centrifugation recover spermatozoa with equally lower amounts of DNA damage than spermatozoa obtained from neat semen.
abstract: We compared the abilities of two commonly used semen preparation techniques to decrease the amount of benzo(a)pyrene-diol-epoxide (BPDE)-DNA adducts in the spermatozoa of ten smokers. Semen processing by swim-up or discontinuous gradient centrifugation recovered spermatozoa showing an equally significantly lower amount of BPDE-DNA adducts than in unprepared spermatozoa from neat semen.
authors.affiliation: Centre d'Étude et de Conservation des Oeufs et du Sperme - Laboratoire de Biologie de la Reproduction, Assistance Publique-Hopitaux de Marseille La Conception, Marseille, France. jeanne.perrin@ap-hm.fr
authors.forename: Jeanne
authors.initials: J
authors.lastname: Perrin
authors.forename: Virginie
authors.initials: V
authors.lastname: Tassistro
authors.forename: Odile
authors.initials: O
authors.lastname: Paulmyer-Lacroix
authors.forename: Blandine
authors.initials: B
authors.lastname: Courbière
authors.forename: Alain
authors.initials: A
authors.lastname: Botta
authors.forename: Irène
authors.initials: I
authors.lastname: Sari-Minodier
mesh_terms: D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide / Q000009:adverse effects*; D000328:Adult; D002499:Centrifugation, Density Gradient; D018736:DNA Adducts; D004249:DNA Damage; D005602:France; D006801:Humans; D008297:Male; D010865:Pilot Projects; D012907:Smoking / Q000009:adverse effects*; D013081:Sperm Motility / Q000187:drug effects*; D053625:Sperm Retrieval; D013094:Spermatozoa / Q000187:drug effects* / Q000473:pathology
chemical_list: D018736:DNA Adducts; D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide