title: Bone-prognostic status after cessation of cadmium exposure for one month in male rats.
abstract: This study investigated bone status after decreased cadmium (Cd) exposure in male rats. Sprague-Dawley male rats were randomly divided into three groups. One group was injected subcutaneously with sodium chloride as control. The others were given CdCl2 by subcutaneous injection at doses of 0.5 mg Cd/kg body weight (bw) for 2 months (Cd+2m) and for 3 months (Cd+3m). For the Cd+2m group, the rats were shifted to cessation of Cd injection for 1 month after 2 months' exposure. At month 3, micro-computed tomography (micro-CT) analyses were performed on the proximal tibia and lumbar spine, and urine was collected from all rats. Rats were then killed and blood collected for metabolic-marker measurement and Cd assay. Bone tissues were also collected for bone-mass assay, biomechanical test, and bone-histology analysis. Cd burdens of rats in the Cd+2m and Cd+3m groups were both significantly greater than those in the control group. Cd burdens of rats were lower in the Cd+2m group compared with the Cd+3m group. Bone damage occurred in the Cd+2m and Cd+3m groups compared with the control group (p<0.05), but no significant improvement was found in the Cd+2m group compared with the Cd+3m group. Cd damage to bone could not be reversed over the short term. More attention should be paid to Cd's toxic effects on bone after decreased exposure.
authors.affiliation: Institute of Radiation Medicine, Fudan University, Xietu Road, Shanghai, 200032, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Chen
authors.forename: Guoying
authors.initials: G
authors.lastname: Zhu
authors.forename: Taiyi
authors.initials: T
authors.lastname: Jin
authors.forename: Chunlin
authors.initials: C
authors.lastname: Shao
authors.forename: Shuzhu
authors.initials: S
authors.lastname: Gu
authors.forename: Mingguang
authors.initials: M
authors.lastname: Tan
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Hanfang
authors.initials: H
authors.lastname: Xiao
authors.forename: Heping
authors.initials: H
authors.lastname: Hu
authors.forename: Yunzhen
authors.initials: Y
authors.lastname: Yu
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001842:Bone and Bones / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D002104:Cadmium / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000633:toxicity*; D004334:Drug Administration Schedule; D004785:Environmental Pollutants / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000633:toxicity*; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D055114:X-Ray Microtomography
chemical_list: D015415:Biomarkers; D004785:Environmental Pollutants; D002104:Cadmium