title: Lipid metabolism abnormalities in alcohol-treated rabbits: a morphometric and haematologic study comparing high and low alcohol doses.
abstract: The pathogenesis of alcohol-induced osteonecrosis remains unclear. The purpose of the present study was to evaluate the morphological changes in bone marrow fat cells and the changes in the serum lipid levels in alcohol-treated rabbits. Fifteen rabbits were randomly assigned into three groups: Four rabbits intragastrically received low-dose alcohol (LDA) (15 ml/kg per day) containing 15% ethanol for 4 weeks, five rabbits received high-dose alcohol (HDA) (30 ml/kg per day) for 4 weeks and six rabbits received physiologic saline for 4 weeks as a control group. Six weeks after the initial alcohol administration, all rabbits were sacrificed. The mean size of the bone marrow fat cells in rabbits treated with HDA was significantly larger than that in the control group (P = 0.0001). Haematologically, the levels of triglycerides and free fatty acids in the rabbits treated with both low-dose and HDA were significantly higher than those in the control group (P = 0.001 for both comparisons). The results of this study are that there are lipid metabolism abnormalities, both morphologically and haematologically, after alcohol administration. Also these findings were more apparent in rabbits treated with HDA than those treated with LDA.
authors.affiliation: Department of Orthopaedic Surgery, Graduate School of Medical Sciences, Kyushu University, Higashi-ku, Fukuoka, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ikemura
authors.forename: Takuaki
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Goro
authors.initials: G
authors.lastname: Motomura
authors.forename: Kenyu
authors.initials: K
authors.lastname: Iwasaki
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Yamaguchi
authors.forename: Garida
authors.initials: G
authors.lastname: Zhao
authors.forename: Yukihide
authors.initials: Y
authors.lastname: Iwamoto
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects / Q000473:pathology*; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000009:adverse effects / Q000494:pharmacology*; D005230:Fatty Acids, Nonesterified / Q000097:blood; D050356:Lipid Metabolism / Q000187:drug effects*; D008055:Lipids / Q000097:blood*; D008297:Male; D023421:Models, Animal; D010020:Osteonecrosis / Q000139:chemically induced / Q000473:pathology*; D011817:Rabbits; D014280:Triglycerides / Q000097:blood
chemical_list: D005230:Fatty Acids, Nonesterified; D008055:Lipids; D014280:Triglycerides; D000431:Ethanol