title: Serum biomarker of diabetic peripheral neuropathy indentified by differential proteomics.
abstract: At least one in four diabetic patients is affected by peripheral neuropathy. In this study, the MALDI-TOF-MS mass spectra of peptides and proteins were generated following WCX CLINPROT bead fractionation of 39 diabetic peripheral neuropathy (DPN), 39 diabetes mellitus (DM), and 35 control (CON) serum samples. The spectra were analyzed statistically using flexAnalysisTM and Clin-ProtTM bioinformatics software. Identification of the selected markers was performed and affinity bead-purified plasma protein was subjected to LTQ Orbitrap XL MS/MS analysis followed by Mascot identification of the peptide sequences. 89 differentially expressed peaks of serum proteins were identified. 17, 10 and 4 most significant peaks between CON vs. DM, CON vs. DPN, DM vs. DPN, respectively, were selected out using the ClinProTool software package and used to train a Supervised Neural Network. A veracity rate of 100% was obtained for all sets. Following this analysis, a 6631-Da marker was identified as a fragment of the Apolipoprotein C-I precursor. The peptides identified may have clinical utility as surrogate markers for detection and classification of DM and DPN.
authors.affiliation: Department of Endocrinology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Tang
authors.forename: Yong-quan
authors.initials: YQ
authors.lastname: Shi
authors.forename: Jun-jie
authors.initials: JJ
authors.lastname: Zou
authors.forename: Xiang-fang
authors.initials: XF
authors.lastname: Chen
authors.forename: Jiao-yang
authors.initials: JY
authors.lastname: Zheng
authors.forename: Shu-wei
authors.initials: SW
authors.lastname: Zhao
authors.forename: Zhi-min
authors.initials: ZM
authors.lastname: Liu
mesh_terms: D000328:Adult; D000368:Aged; D053302:Apolipoprotein C-I / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D003920:Diabetes Mellitus / Q000097:blood / Q000175:diagnosis; D003929:Diabetic Neuropathies / Q000097:blood* / Q000175:diagnosis; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010446:Peptide Fragments / Q000097:blood; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D053302:Apolipoprotein C-I; D015415:Biomarkers; D010446:Peptide Fragments