title: UV B-irradiation enhances the racemization and isomerizaiton of aspartyl residues and production of Nε-carboxymethyl lysine (CML) in keratin of skin.
abstract: UV-B irradiation is one of the risk factors in age-related diseases. We have reported that biologically uncommon D-β-Asp residues accumulate in proteins from sun-exposed elderly human skin. A previous study also reported that carboxymethyl lysine (CML; one of the advanced glycation end products (AGEs)) which is produced by the oxidation of glucose and peroxidation of lipid, also increases upon UV B irradiation. The formation of D-β-Asp and CML were reported as the alteration of proteins in UV B irradiated skin, independently. In this study, in order to clarify the relationship between the formation of D-β-Asp and CML, immunohistochemical analysis using anti-D-β-Asp containing peptide antibodies and anti-CML antibodies was performed in UV B irradiated mice. Immunohistochemical analyses clearly indicated that an anti-D-β-Asp containing peptide antibody and anti-CML antibody reacted at a common area in UV B irradiated skin. Western blot analyses of the proteins isolated from UV B irradiated skin demonstrated that proteins of 50-70 kDa were immunoreactive towards antibodies for both D-β-Asp containing peptide and CML. These proteins were identified by proteomic analysis as members of the keratin families including keratin-1, keratin-6B, keratin-10, and keratin-14.
authors.affiliation: Research Reactor Institute, Kyoto University, Kumatori, Osaka 590-0494, Japan.
authors.forename: Yuhei
authors.initials: Y
authors.lastname: Mori
authors.forename: Kenzo
authors.initials: K
authors.lastname: Aki
authors.forename: Katsunori
authors.initials: K
authors.lastname: Kuge
authors.forename: Shingo
authors.initials: S
authors.lastname: Tajima
authors.forename: Natsuko
authors.initials: N
authors.lastname: Yamanaka
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Kaji
authors.forename: Naoki
authors.initials: N
authors.lastname: Yamamoto
authors.forename: Ryoji
authors.initials: R
authors.lastname: Nagai
authors.forename: Hanako
authors.initials: H
authors.lastname: Yoshii
authors.forename: Norihiko
authors.initials: N
authors.lastname: Fujii
authors.forename: Masami
authors.initials: M
authors.lastname: Watanabe
authors.forename: Tadatoshi
authors.initials: T
authors.lastname: Kinouchi
authors.forename: Noriko
authors.initials: N
authors.lastname: Fujii
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D000906:Antibodies / Q000737:chemistry; D015153:Blotting, Western; D026603:D-Aspartic Acid / Q000032:analysis / Q000737:chemistry / Q000528:radiation effects*; D017127:Glycation End Products, Advanced / Q000378:metabolism / Q000528:radiation effects; D006801:Humans; D007150:Immunohistochemistry; D007633:Keratins / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects*; D008239:Lysine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000528:radiation effects; D051379:Mice; D040901:Proteomics; D012867:Skin / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects*; D013237:Stereoisomerism; D014466:Ultraviolet Rays
chemical_list: D000906:Antibodies; D017127:Glycation End Products, Advanced; D026603:D-Aspartic Acid; D007633:Keratins; C048496:N(6)-carboxymethyllysine; D008239:Lysine