title: Effect of the bone marrow derived-mononuclear stem cells transplantation in the growth, VEGF-R and TNF-alpha expression of endometrial implants in Wistar rats.
abstract: OBJECTIVE
  To study the effect of bone marrow derived-mononuclear stem cells transplantation in the growth, VEGF-R and TNF-alpha expression of surgically induced endometriosis in an experimental model.
  
  
  METHODS
  This is an experimental study conducted in the Center for Health and Biological Sciences at the Pontifical Catholic University of Parana, Brazil. Endometriotic implants were surgically induced in 120 female Wistar rats. The animals with viable endometrial implant (larger than 25 mm(2)) were randomically divided into 3 groups to receive an intraperitoneal injection of 0.2 cc of saline solution (C group; n=30), a subcutaneous injection of 1mg/kg of leuprolide (L group; n=34), or an intraperitoneal injection of 5×10(6) bone marrow derived-mononuclear stem cells (SC group; n=36). They were sacrificed after 21 days to assess the implants' size and the tissue expression of vascular endothelial growth factor receptor (VEGF-R) and tumor necrosis factor-alpha (TNF-alpha).
  
  
  RESULTS
  Treatment with leuprolide decreased the surface area of the endometriotic implant compared to the SC group and the C group. The absolute reduction in the surface area of the implant was 16.5mm, 0mm, and 0mm (p=0.007), respectively, and the percent reduction was 40.2%, 0%, and 0% (p=0.001). VEGF-R expression in the endometriotic implant decreased after treatment in the L and SC groups compared to the C group (409.6 μm(2) vs. 465 μm(2) vs. 920.9 μm(2), respectively; p=0.021). TNF-alpha expression also reduced in the L and SC groups compared to the C group (585.7 μm(2) vs. 549.3 μm(2) vs. 2402.1 μm(2), respectively; p<0.001).
  
  
  CONCLUSIONS
  Bone marrow derived-mononuclear stem cells transplantation decreased the expression of VEGF-R and TNF-alpha in the endometriotic implant but did not reduce the surface area of the lesion.
authors.affiliation: Department Center for Health and Biological Sciences, Pontifical Catholic University of Parana (PUC-PR), Brazil. williamkondo@yahoo.com
authors.forename: William
authors.initials: W
authors.lastname: Kondo
authors.forename: Eduardo Andreazza
authors.initials: EA
authors.lastname: Dal Lago
authors.forename: Julio Cesar
authors.initials: JC
authors.lastname: Francisco
authors.forename: Rossana Baggio
authors.initials: RB
authors.lastname: Simeoni
authors.forename: Lucia
authors.initials: L
authors.lastname: de Noronha
authors.forename: Ana Paula Camargo
authors.initials: AP
authors.lastname: Martins
authors.forename: Marina Luise Viola
authors.initials: ML
authors.lastname: de Azevedo
authors.forename: Carolina Chaves
authors.initials: CC
authors.lastname: Ferreira
authors.forename: Priscila
authors.initials: P
authors.lastname: Maestrelli
authors.forename: Marcia
authors.initials: M
authors.lastname: Olandoski
authors.forename: Luiz Cesar
authors.initials: LC
authors.lastname: Guarita-Souza
authors.forename: Vivian Ferreira
authors.initials: VF
authors.lastname: do Amaral
mesh_terms: D000818:Animals; D001853:Bone Marrow; D004195:Disease Models, Animal; D004715:Endometriosis / Q000378:metabolism* / Q000473:pathology / Q000628:therapy*; D005260:Female; D005300:Fertility Agents, Female / Q000008:administration & dosage; D007274:Injections, Intraperitoneal; D016729:Leuprolide / Q000008:administration & dosage; D051381:Rats; D017208:Rats, Wistar; D040262:Receptors, Vascular Endothelial Growth Factor / Q000096:biosynthesis*; D033581:Stem Cell Transplantation; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
chemical_list: D005300:Fertility Agents, Female; D014409:Tumor Necrosis Factor-alpha; D040262:Receptors, Vascular Endothelial Growth Factor; D016729:Leuprolide