title: New approaches to identification and activity estimation of glyphosate degradation enzymes.
abstract: We propose a new set of approaches, which allow identifying the primary enzymes of glyphosate (N-phosphonomethyl-glycine) attack, measuring their activities, and quantitative analysis of glyphosate degradation in vivo and in vitro. Using the developed approach we show that glyphosate degradation can follow different pathways depending on physiological characteristics of metabolizing strains: in Ochrobactrum anthropi GPK3 the initial cleavage reaction is catalyzed by glyphosate-oxidoreductase with the formation of aminomethylphosphonic acid and glyoxylate, whereas Achromobacter sp. MPS12 utilize C-P lyase, forming sarcosine. The proposed methodology has several advantages as compared to others described in the literature.
authors.affiliation: Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia. alhummen@rambler.ru
authors.forename: A V
authors.initials: AV
authors.lastname: Sviridov
authors.forename: N F
authors.initials: NF
authors.lastname: Zelenkova
authors.forename: N G
authors.initials: NG
authors.lastname: Vinokurova
authors.forename: I T
authors.initials: IT
authors.lastname: Ermakova
authors.forename: A A
authors.initials: AA
authors.lastname: Leontievsky
mesh_terms: D042402:Achromobacter / Q000201:enzymology; D002851:Chromatography, High Pressure Liquid; D005998:Glycine / Q000031:analogs & derivatives* / Q000378:metabolism; D007555:Isoxazoles; D008190:Lyases / Q000378:metabolism*; D020621:Ochrobactrum anthropi / Q000201:enzymology; D063065:Organophosphonates / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism*; D012521:Sarcosine / Q000378:metabolism; D013777:Tetrazoles; D000097797:Glyphosate
chemical_list: D005998:Glycine; D007555:Isoxazoles; D008190:Lyases; D063065:Organophosphonates; D010088:Oxidoreductases; D012521:Sarcosine; D013777:Tetrazoles; D000097797:Glyphosate; C051328:carbon-phosphorus lyase; C418233:2-amino-3-(3-hydrox-5(2-methyl-2H-tetrazol-5-yl)isoxazol-4-yl)propionic acid