title: Development of a novel flow injection liquid-liquid microextraction method for the on-line separation and preconcentration for determination of zinc(II) using 5-(8-hydroxy-2-quinolinylmethyl)-2,8-dithia-5-aza-2,6-pyridinophane as a sensitive and selective fluorescent chemosensor.
abstract: A novel flow injection analysis (FIA) system based on liquid-liquid microextraction and fluorimetric determination was developed for the determination of traces of the Zn(2+) ion using 5-(8-hydroxy-2-quinolinylmethyl)-2,8-dithia-5-aza-2,6-pyridinophane (L) as a sensitive and selective fluorimetric sensor, with λ(ex)=373 nm and λ(em)=530 nm, and hexanol as the extracting organic solvent. In the designed FIA system, the phase separation takes place via gravitation forces in the absence of any segmenter. The influence of pH and ionic strength of the solution, amount of ligand, nature of counter ion, volume of organic solvent, extraction time and coil length was investigated. Under optimized experimental conditions, the calibration curve found to be liner over a concentration range of 0.025-4.53 μg mL(-1) (R(2)=0.9951) with a limit of detection of 2.3 ng mL(-1). The enrichment factor was 45 and relative standard deviation for 7 replicate determinations was 2.43%. The method is very fast and uses low levels of organic solvents. The proposed method was applied successfully to the determination of zinc(II) in human hair, human serum and two inorganic sludge samples.
authors.affiliation: Department of Chemistry, Razi University, Kermanshah, Iran. mshamsipur@yahoo.com
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Shamsipur
authors.forename: Mir Mahdi
authors.initials: MM
authors.lastname: Zahedi
authors.forename: Greta
authors.initials: G
authors.lastname: De Filippo
authors.forename: Vito
authors.initials: V
authors.lastname: Lippolis
mesh_terms: D001372:Aza Compounds; D001952:Bridged-Ring Compounds; D017022:Flow Injection Analysis / Q000379:methods*; D005453:Fluorescence; D006197:Hair / Q000737:chemistry; D006801:Humans; D008722:Methods; D044967:Serum / Q000737:chemistry; D012722:Sewage / Q000737:chemistry; D013054:Spectrophotometry, Atomic / Q000379:methods*; D015032:Zinc / Q000032:analysis* / Q000302:isolation & purification
chemical_list: D001372:Aza Compounds; D001952:Bridged-Ring Compounds; D012722:Sewage; C068781:2,6-pyridinophane; D015032:Zinc