title: A one step genotypic identification of Lactococcus lactis subspecies at the species/strain levels.
abstract: A Lactococcus lactis subspecies-specific primer was designed based on their repetitive genome sequences. This primer enabled L. lactis subspecies to be identified simultaneously at both the species level and also the strain level. Based on studies using 70 strains of L. lactis and 60 strains of other non-target bacteria, the identification completely matched that obtained by the sequence of the 16S rRNA gene. However, inconsistency between phenotypic and genotypic characteristics was observed in some strains isolated from milk.
authors.affiliation: Food Science and Technology Institute, Morinaga Milk Industry Co., Ltd., Zama, Kanagawa 228-8583, Japan. t-odamak@morinagamilk.co.jp
authors.forename: Toshitaka
authors.initials: T
authors.lastname: Odamaki
authors.forename: Sumiko
authors.initials: S
authors.lastname: Yonezawa
authors.forename: Hirosuke
authors.initials: H
authors.lastname: Sugahara
authors.forename: Jin-zhong
authors.initials: JZ
authors.lastname: Xiao
authors.forename: Tomoko
authors.initials: T
authors.lastname: Yaeshima
authors.forename: Keiji
authors.initials: K
authors.lastname: Iwatsuki
mesh_terms: D000818:Animals; D001483:Base Sequence; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005798:Genes, Bacterial; D014644:Genetic Variation; D016680:Genome, Bacterial; D005838:Genotype; D013294:Lactococcus lactis / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D008892:Milk / Q000382:microbiology; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S