title: Oxidative stress, cytoxicity, and cell mortality induced by nano-sized lead in aqueous suspensions.
abstract: This paper reports on the effect of aqueous and nano-particulated Pb on oxidative stress (lipid peroxidation), cytoxicity, and cell mortality. As determined by the Thiobarbituric Acid Reactive Substances (TBARS) method, only 6h after incubation aqueous suspensions bearing nano-sized PbO(2), soluble Pb(II), and brain-homogenate only suspensions, were determined to contain as much as ca. 7, 5, and 1 nmol TBARS mg protein(-1), respectively. Exposure of human cells (central nervous system, prostate, leukemia, colon, breast, lung cells) to nano-PbO(2) led to cell-growth inhibition values (%) ca. ≤18.7%. Finally, as estimated by the Artemia salina test, cell mortality values were found to show high-survival larvae rates. Microscopic observations revealed that Pb particles were swallowed, but caused no mortality, however.
authors.affiliation: Departamento de Procesos y Tecnología, Universidad Autónoma Metropolitana Unidad Cuajimalpa, Mexico.
authors.forename: Hilda
authors.initials: H
authors.lastname: Cornejo-Garrido
authors.forename: Daria
authors.initials: D
authors.lastname: Kibanova
authors.forename: Antonio
authors.initials: A
authors.lastname: Nieto-Camacho
authors.forename: José
authors.initials: J
authors.lastname: Guzmán
authors.forename: Teresa
authors.initials: T
authors.lastname: Ramírez-Apan
authors.forename: Pilar
authors.initials: P
authors.lastname: Fernández-Lomelín
authors.forename: Maria Laura
authors.initials: ML
authors.lastname: Garduño
authors.forename: Javiera
authors.initials: J
authors.lastname: Cervini-Silva
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003603:Cytotoxins / Q000633:toxicity*; D006801:Humans; D007854:Lead / Q000633:toxicity*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D053768:Metal Nanoparticles / Q000633:toxicity*; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D003603:Cytotoxins; D017392:Thiobarbituric Acid Reactive Substances; D014874:Water Pollutants, Chemical; D007854:Lead; D008315:Malondialdehyde