title: Application of non-structural protein ELISA kits in nationwide FMD surveillance in pigs to demonstrate virus circulation in Taiwan.
abstract: Large scale surveillance of FMD non-structural protein (NSP) antibody in pigs was conducted to monitor for FMD virus circulation in Taiwan using Ceditest and UBI NSP ELISA kits after recurrence of FMD in 2009. A total of 53,759 serum samples were collected from pigs in the auction markets in 2009. There were 43 farms with positive FMD NSP reactors to both NSP ELISA tests in the nationwide surveillance. After tracing back, clinical examination and the NSP ELISA testing using both Ceditest and UBI on 14 follow-up serum samples from all the herds with confirmed NSP reactors in 2009, there were 4 farms classified as positive on follow-up testing criteria. In this surveillance, we have demonstrated that the NSP ELISA tests of outbreak farms followed by clinical and serological investigation could be used to detect FMD circulation in the pig population in Taiwan even while the national compulsory vaccination program is ongoing.
authors.affiliation: Division of Animal Medicine, Animal Technology Institute Taiwan, Chunan, Miaoli, Taiwan.
authors.forename: S P
authors.initials: SP
authors.lastname: Chen
authors.forename: M C
authors.initials: MC
authors.lastname: Lee
authors.forename: Y F
authors.initials: YF
authors.lastname: Sun
authors.forename: P C
authors.initials: PC
authors.lastname: Yang
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D004196:Disease Outbreaks / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000662:veterinary; D005536:Foot-and-Mouth Disease / Q000453:epidemiology / Q000821:virology*; D029544:Foot-and-Mouth Disease Virus / Q000302:isolation & purification*; D034421:Sus scrofa; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology*; D013624:Taiwan / Q000453:epidemiology; D017361:Viral Nonstructural Proteins / Q000302:isolation & purification
chemical_list: D000914:Antibodies, Viral; D017361:Viral Nonstructural Proteins