title: Laser Vaporization/Ionization Interface for Capillary Electrophoresis-Time-of-Flight Mass Spectrometry.
abstract: Mass spectrometry (MS) is usually coupled on-line with capillary electrophoresis (CE) to analyze biomolecules by using electrospray ionization or continuous-flow fast-atom bombardment. We present a new design for laser vaporization/ionization time-of-flight mass spectrometry. CE, with its low flow rate (<1 μL/min), is highly compatible with MS, even if the total column effluent is introduced directly. A UV laser is used to vaporize and ionize the solution eluting from the column. There is no need to have a makeup solvent. Using this system, we have analyzed a group of amines and peptides. The concentration detection limit of serotonin is in the 10(-)(7) M level. The separation and identification of an amine mixture by CE/MS demonstrates the complementary nature of the information.
authors.affiliation: Ames Laboratory [Formula: see text] USDOE and Department of Chemistry, Iowa State University, Ames, Iowa 50011.
authors.forename: S Y
authors.initials: SY
authors.lastname: Chang
authors.forename: E S
authors.initials: ES
authors.lastname: Yeung