title: Pre-microRNA and mature microRNA in human mitochondria.
abstract: BACKGROUND
  Because of the central functions of the mitochondria in providing metabolic energy and initiating apoptosis on one hand and the role that microRNA (miRNA) play in gene expression, we hypothesized that some miRNA could be present in the mitochondria for post-transcriptomic regulation by RNA interference. We intend to identify miRNA localized in the mitochondria isolated from human skeletal primary muscular cells.
  
  
  RESULTS
  To investigate the potential origin of mitochondrial miRNA, we in-silico searched for microRNA candidates in the mtDNA. Twenty five human pre-miRNA and 33 miRNA aligments (E-value<0.1) were found in the reference mitochondrial sequence and some of the best candidates were chosen for a co-localization test. In situ hybridization of pre-mir-302a, pre-let-7b and mir-365, using specific labelled locked nucleic acids and confocal microscopy, demonstrated that these miRNA were localized in mitochondria of human myoblasts. Total RNA was extracted from enriched mitochondria isolated by an immunomagnetic method from a culture of human myotubes. The detection of 742 human miRNA (miRBase) were monitored by RT-qPCR at three increasing mtRNA inputs. Forty six miRNA were significantly expressed (2(nd) derivative method Cp>35) for the smallest RNA input concentration and 204 miRNA for the maximum RNA input concentration. In silico analysis predicted 80 putative miRNA target sites in the mitochondrial genome (E-value<0.05).
  
  
  CONCLUSIONS
  The present study experimentally demonstrated for the first time the presence of pre-miRNA and miRNA in the human mitochondria isolated from skeletal muscular cells. A set of miRNA were significantly detected in mitochondria fraction. The origin of these pre-miRNA and miRNA should be further investigate to determine if they are imported from the cytosol and/or if they are partially processed in the mitochondria.
authors.affiliation: Unité de Biologie Intégrative des Adaptations à l'Exercice-INSERM U902, Genopole Evry, France. eric.barrey@inserm.fr
authors.forename: Eric
authors.initials: E
authors.lastname: Barrey
authors.forename: Gaelle
authors.initials: G
authors.lastname: Saint-Auret
authors.forename: Blandine
authors.initials: B
authors.lastname: Bonnamy
authors.forename: Dominique
authors.initials: D
authors.lastname: Damas
authors.forename: Orane
authors.initials: O
authors.lastname: Boyer
authors.forename: Xavier
authors.initials: X
authors.lastname: Gidrol
mesh_terms: D001483:Base Sequence; D015342:DNA Probes / Q000378:metabolism; D004272:DNA, Mitochondrial / Q000235:genetics; D004076:Digoxigenin / Q000378:metabolism; D054629:Genome, Mitochondrial / Q000235:genetics; D006801:Humans; D017403:In Situ Hybridization; D035683:MicroRNAs / Q000235:genetics*; D044085:Microfluidics; D008928:Mitochondria / Q000235:genetics*; D008969:Molecular Sequence Data; D032446:Myoblasts / Q000166:cytology / Q000378:metabolism; D009841:Oligonucleotides / Q000235:genetics; D012322:RNA Precursors / Q000235:genetics*
chemical_list: D015342:DNA Probes; D004272:DNA, Mitochondrial; D035683:MicroRNAs; D009841:Oligonucleotides; D012322:RNA Precursors; C477371:locked nucleic acid; D004076:Digoxigenin