title: Interleukin-8-251T > A, Interleukin-1α-889C > T and Apolipoprotein E polymorphisms in Alzheimer's disease.
abstract: An inflammatory process has been involved in numerous neurodegenerative disorders such as Parkinson's disease, stroke and Alzheimer's disease (AD). In AD, the inflammatory response is mainly located in the vicinity of amyloid plaques. Cytokines, such as interleukin-8 (IL-8) and interleukin-1α (IL-1α), have been clearly involved in this inflammatory process. Polymorphisms of several interleukin genes have been correlated to the risk of developing AD. The present study investigated the association of AD with polymorphisms IL-8 -251T > A (rs4073) and IL-1α-889C > T (rs1800587) and the interactive effect of both, adjusted by the Apolipoprotein E genotype. 199 blood samples from patients with AD, 146 healthy elderly controls and 95 healthy young controls were obtained. DNA samples were isolated from blood cells, and the PCR-RFLP method was used for genotyping. The genotype distributions of polymorphisms IL-8, IL-1α and APOE were as expected under Hardy-Weinberg equilibrium. The allele frequencies did not differ significantly among the three groups tested. As expected, the APOE4 allele was strongly associated with AD (p < 0.001). No association of AD with either the IL-1α or the IL-8 polymorphism was observed, nor was any interactive effect between both polymorphisms. These results confirm previous studies in other populations, in which polymorphisms IL-8 -251T > A and IL-1α-889C > T were not found to be risk factors for AD.
authors.affiliation: Pós-Graduação em Biologia Oral, Universidade do Sagrado Coração, Bauru, SP, Brazil.
authors.forename: Alex Augusto
authors.initials: AA
authors.lastname: Vendramini
authors.forename: Roger Willian
authors.initials: RW
authors.lastname: de Lábio
authors.forename: Lucas Trevizani
authors.initials: LT
authors.lastname: Rasmussen
authors.forename: Nathali Mattiuzo
authors.initials: NM
authors.lastname: Dos Reis
authors.forename: Thais
authors.initials: T
authors.lastname: Minett
authors.forename: Paulo Henrique Ferreira
authors.initials: PH
authors.lastname: Bertolucci
authors.forename: Marcela Augusta
authors.initials: MA
authors.lastname: de Souza Pinhel
authors.forename: Dorotéia Rossi Silva
authors.initials: DR
authors.lastname: Souza
authors.forename: Diego Robles
authors.initials: DR
authors.lastname: Mazzotti
authors.forename: Marília
authors.initials: M
authors.lastname: de Arruda Cardoso Smith
authors.forename: Spencer Luiz Marques
authors.initials: SL
authors.lastname: Payão