title: Covalent immobilization of active lysozyme on Si/glass surface using alkoxy Fischer carbene complex on SAM.
abstract: A cross-metathesis reaction between an alkene terminated self-assembled monolayer (SAM) on glass/Si wafer and an alkene tethered Fischer carbene complex yielded a functionalized surface. Rapid aminolysis of the Fischer carbene moieties permit efficient anchoring of amine containing molecules on such a surface. Attachment of 1-pyrenemethylamine was thus monitored by ATR-IR spectroscopy and fluorescence microscopy. Similarly, BSA and lysozyme were individually grafted to such Fischer carbene modified surfaces using their pendant lysine residues. It has been demonstrated that the anchored lysozyme retains its bactericidal property.
authors.affiliation: Department of Organic Chemistry, Indian Association for the Cultivation of Science, Kolkata-700032, India.
authors.forename: Piyali
authors.initials: P
authors.lastname: Dutta
authors.forename: Namrata
authors.initials: N
authors.lastname: Ray
authors.forename: Sarita
authors.initials: S
authors.lastname: Roy
authors.forename: Anjan Kr
authors.initials: AK
authors.lastname: Dasgupta
authors.forename: Othman
authors.initials: O
authors.lastname: Bouloussa
authors.forename: Amitabha
authors.initials: A
authors.lastname: Sarkar
mesh_terms: D000475:Alkenes / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005898:Glass / Q000737:chemistry; D008567:Membranes, Artificial; D008697:Methane / Q000031:analogs & derivatives* / Q000737:chemistry; D015394:Molecular Structure; D009113:Muramidase / Q000737:chemistry* / Q000378:metabolism; D017646:Organosilicon Compounds / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013499:Surface Properties
chemical_list: D000475:Alkenes; D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D017646:Organosilicon Compounds; C030011:carbene; D012710:Serum Albumin, Bovine; D009113:Muramidase; D008697:Methane