title: Temporal genetic variability and host sources of Escherichia coli associated with fecal pollution from domesticated animals in the shellfish culture environment of Xiangshan Bay, East China Sea.
abstract: This study was conducted to analyze the genetic variability of Escherichia coli from domesticated animal wastes for microbial source tracking (MST) application in fecal contaminated shellfish growing waters of Xiangshan Bay, East China Sea. (GTG)(5) primer was used to generate 1363 fingerprints from E. coli isolated from feces of known 9 domesticated animal sources around this shellfish culture area. Jackknife analysis of the complete (GTG)(5)-PCR DNA fingerprint library indicated that isolates were assigned to the correct source groups with an 84.28% average rate of correct classification. Based on one-year source tracking data, the dominant sources of E. coli were swine, chickens, ducks and cows in this water area. Moreover, annual and spatial changes of E. coli concentrations and host sources may affect the level and distribution of zoonotic pathogen species in waters. Our findings will further contribute to preventing fecal pollution in aquatic environments and quality control of shellfish.
authors.affiliation: Food Safety Key Laboratory of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310035, PR China. full1103@yahoo.com.cn
authors.forename: Ling-Lin
authors.initials: LL
authors.lastname: Fu
authors.forename: Jiang-Bing
authors.initials: JB
authors.lastname: Shuai
authors.forename: Yanbo
authors.initials: Y
authors.lastname: Wang
authors.forename: Hong-Jia
authors.initials: HJ
authors.lastname: Ma
authors.forename: Jian-Rong
authors.initials: JR
authors.lastname: Li
mesh_terms: D000822:Animal Husbandry; D000818:Animals; D000829:Animals, Domestic / Q000382:microbiology; D017756:Aquaculture / Q000706:statistics & numerical data; D061006:Bays / Q000737:chemistry / Q000382:microbiology*; D002681:China; D004784:Environmental Monitoring / Q000379:methods; D004926:Escherichia coli / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D005243:Feces / Q000382:microbiology*; D009792:Oceans and Seas; D012758:Shellfish / Q000382:microbiology* / Q000706:statistics & numerical data; D014871:Water Microbiology; D014876:Water Pollution / Q000706:statistics & numerical data