title: Molecular cloning, tissue distribution and ontogenetic expression of Xiang pig chemerin and its involvement in regulating energy metabolism through Akt and ERK1/2 signaling pathways.
abstract: Chemerin, as a new member of adipokines family, is highly expressed in adipose tissue in rodent and its expression increases with obesity. Moreover, chemerin has been reported to have significant relationship with metabolic syndrome and insulin sensitivity. Here, the gene encoding chemerin from Xiang pig was cloned. The open reading frame of this cDNA encodes 163 deduced amino acid residues. The putative protein has a N-terminal signaling peptide and a nuclear localization signal profile which are highly conserved among the vertebrate orthologs. Both chemerin and chemerinR are highly expressed in lung, kidney and small intestine in adult Xiang pig. Besides these tissues, chemerin is abundant in liver and backfat, and chemerinR is abundant in spleen and skeletal muscle. We also investigated the age-dependent expression of chemerin in suckling Xiang piglets in various tissues, which showed an interaction between age and segments in abundance of chemerin and chemerinR from day 1 to day 21. For chemerinR, it was abundant in skeletal muscle of both adult and fetal Xiang pig. Further, we treated differentiated C2C12 cells with chemerin. The result showed that chemerin regulated energy metabolism partly through Akt and ERK1/2 signaling pathway. Taken together, our findings provide basic molecular information for the deeper investigation on the function of chemerin.
authors.affiliation: Research Center for Healthy Breeding of Livestock and Poultry, Hunan Engineering and Research Center of Animal and Poultry Science and Key Laboratory for Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, the Chinese Academy of Sciences, Hunan 410125, China.
authors.forename: Huansheng
authors.initials: H
authors.lastname: Yang
authors.forename: Fengna
authors.initials: F
authors.lastname: Li
authors.forename: Xiangfeng
authors.initials: X
authors.lastname: Kong
authors.forename: Xiaoxue
authors.initials: X
authors.lastname: Yuan
authors.forename: Guoqi
authors.initials: G
authors.lastname: Lian
authors.forename: Meimei
authors.initials: M
authors.lastname: Geng
authors.forename: Tiejun
authors.initials: T
authors.lastname: Li
authors.forename: Jingdong
authors.initials: J
authors.lastname: Yin
authors.forename: Yulong
authors.initials: Y
authors.lastname: Yin
mesh_terms: D054392:Adipokines / Q000235:genetics* / Q000378:metabolism* / Q000494:pharmacology; D000367:Age Factors; D000704:Analysis of Variance; D000818:Animals; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002630:Chemotactic Factors / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D019295:Computational Biology; D004734:Energy Metabolism / Q000187:drug effects* / Q000502:physiology; D015870:Gene Expression; D020869:Gene Expression Profiling; D020935:MAP Kinase Signaling System / Q000502:physiology; D051379:Mice; D016366:Open Reading Frames / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D034421:Sus scrofa / Q000235:genetics*
chemical_list: D054392:Adipokines; D002630:Chemotactic Factors