title: The ImmunoCAP ISAC molecular allergology approach in adult multi-sensitized Italian patients with respiratory symptoms.
abstract: OBJECTIVE
  To evaluate the performances of an allergen microarray in multi-sensitized allergic patients with respiratory symptoms.
  
  
  METHODS
  321 patients and 92 controls were included in this study. Specific serum IgE were assayed using ImmunoCAP ISAC, a microarray containing 103 components derived from 47 allergens and results were compared with extract-based ImmunoCAP Allergens sIgE to 15 common airborne allergens.
  
  
  RESULTS
  The reproducibility of ISAC was good. The Positive Percent Agreement (PPA) varied between 75% and 100% for sIgE levels above 1 kUA/l. For samples with sIgE levels below 0.1 kUA/l, the Negative Percent Agreement (NPA) ranged between 90% and 100%. Notably, 58% of respiratory allergy patients had IgE to food-specific proteins and 52% resulted sensitized to cross-reactive pan-allergens.
  
  
  CONCLUSIONS
  ImmunoCAP ISAC detects allergen sensitization at component level and adds important information by defining both cross and co-sensitization to a large variety of allergen molecules.
authors.affiliation: Dipartimento di Medicina Sperimentale, UO Laboratorio di Analisi, Istituto Giannina Gaslini, Genoa, Italy. giovannimelioli@gmail.com
authors.forename: Giovanni
authors.initials: G
authors.lastname: Melioli
authors.forename: Floriano
authors.initials: F
authors.lastname: Bonifazi
authors.forename: Sergio
authors.initials: S
authors.lastname: Bonini
authors.forename: Enrico
authors.initials: E
authors.lastname: Maggi
authors.forename: Michele
authors.initials: M
authors.lastname: Mussap
authors.forename: Gianni
authors.initials: G
authors.lastname: Passalacqua
authors.forename: Enzo Renato
authors.initials: ER
authors.lastname: Rossi
authors.forename: Angelo
authors.initials: A
authors.lastname: Vacca
authors.forename: Giorgio Walter
authors.initials: GW
authors.lastname: Canonica
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000485:Allergens / Q000032:analysis* / Q000276:immunology; D016022:Case-Control Studies; D003429:Cross Reactions; D005260:Female; D006801:Humans; D006967:Hypersensitivity / Q000150:complications / Q000175:diagnosis*; D007073:Immunoglobulin E / Q000097:blood; D008297:Male; D008875:Middle Aged; D040081:Protein Array Analysis / Q000379:methods; D015203:Reproducibility of Results; D012140:Respiratory Tract Diseases / Q000209:etiology; D012882:Skin Tests / Q000379:methods*; D055815:Young Adult
chemical_list: D000485:Allergens; D007073:Immunoglobulin E