title: Lymphoadenopathy during lyme borreliosis is caused by spirochete migration-induced specific B cell activation.
abstract: Lymphadenopathy is a hallmark of acute infection with Borrelia burgdorferi, a tick-borne spirochete and causative agent of Lyme borreliosis, but the underlying causes and the functional consequences of this lymph node enlargement have not been revealed. The present study demonstrates that extracellular, live spirochetes accumulate in the cortical areas of lymph nodes following infection of mice with either host-adapted, or tick-borne B. burgdorferi and that they, but not inactivated spirochetes, drive the lymphadenopathy. The ensuing lymph node response is characterized by strong, rapid extrafollicular B cell proliferation and differentiation to plasma cells, as assessed by immunohistochemistry, flow cytometry and ELISPOT analysis, while germinal center reactions were not consistently observed. The extrafollicular nature of this B cell response and its strongly IgM-skewed isotype profile bear the hallmarks of a T-independent response. The induced B cell response does appear, however, to be largely antigen-specific. Use of a cocktail of recombinant, in vivo-expressed B. burgdorferi-antigens revealed the robust induction of borrelia-specific antibody-secreting cells by ELISPOT. Furthermore, nearly a quarter of hybridomas generated from regional lymph nodes during acute infection showed reactivity against a small number of recombinant Borrelia-antigens. Finally, neither the quality nor the magnitude of the B cell responses was altered in mice lacking the Toll-like receptor adaptor molecule MyD88. Together, these findings suggest a novel evasion strategy for B. burgdorferi: subversion of the quality of a strongly induced, potentially protective borrelia-specific antibody response via B. burdorferi's accumulation in lymph nodes.
authors.affiliation: Center for Comparative Medicine, University of California Davis, Davis, California, United States of America.
authors.forename: Stefan S
authors.initials: SS
authors.lastname: Tunev
authors.forename: Christine J
authors.initials: CJ
authors.lastname: Hastey
authors.forename: Emir
authors.initials: E
authors.lastname: Hodzic
authors.forename: Sunlian
authors.initials: S
authors.lastname: Feng
authors.forename: Stephen W
authors.initials: SW
authors.lastname: Barthold
authors.forename: Nicole
authors.initials: N
authors.lastname: Baumgarth
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000378:metabolism; D000942:Antigens, Bacterial / Q000378:metabolism; D001402:B-Lymphocytes / Q000378:metabolism / Q000382:microbiology* / Q000473:pathology*; D025065:Borrelia burgdorferi / Q000302:isolation & purification / Q000502:physiology*; D049109:Cell Proliferation; D004195:Disease Models, Animal; D005260:Female; D008193:Lyme Disease / Q000150:complications* / Q000378:metabolism / Q000473:pathology; D008198:Lymph Nodes / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D008206:Lymphatic Diseases / Q000378:metabolism / Q000382:microbiology* / Q000473:pathology; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D016513:Mice, SCID; D053594:Myeloid Differentiation Factor 88 / Q000378:metabolism; D013144:Spirochaetales / Q000276:immunology / Q000502:physiology*; D013987:Ticks / Q000382:microbiology
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D053594:Myeloid Differentiation Factor 88