title: Use of modified PCR ribotyping for direct detection of Clostridium difficile ribotypes in stool samples.
abstract: PCR ribotyping was modified to allow direct detection of Clostridium difficile from stool samples. Direct PCR ribotyping was possible in 86 out of 99 C. difficile-positive stool samples, and in 84 cases (84.8%), the ribotype determined directly from the stool sample was identical to the ribotype of the strain isolated from the same stool sample.
authors.affiliation: Institute of Public Health Maribor, Prvomajska 1, 2000 Maribor, Slovenia.
authors.forename: Sandra
authors.initials: S
authors.lastname: Janezic
authors.forename: Iztok
authors.initials: I
authors.lastname: Strumbelj
authors.forename: Maja
authors.initials: M
authors.lastname: Rupnik
mesh_terms: D016360:Clostridioides difficile / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D003015:Clostridium Infections / Q000175:diagnosis* / Q000382:microbiology; D005243:Feces / Q000382:microbiology*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D021521:Ribotyping / Q000379:methods*