title: A luminescent oxygen channeling biosensor that measures small GTPase activation.
abstract: We established a homogeneous luminescent oxygen channeling sensor for measuring activation states of small GTPases. The assay quantifies activated GTPases in cell lysates, can be applied to different GTPases, and can be used for multiplex screening. The study will provide guidelines for determining activation states of diverse GTPases in various biological contexts.
authors.affiliation: LIMES Institute, Chemical Biology & Medicinal Chemistry Unit, University of Bonn, 53121 Bonn, Germany.
authors.forename: Björn
authors.initials: B
authors.lastname: Niebel
authors.forename: Benjamin
authors.initials: B
authors.lastname: Weiche
authors.forename: Alan L
authors.initials: AL
authors.lastname: Mueller
authors.forename: Dean Y
authors.initials: DY
authors.lastname: Li
authors.forename: Nora
authors.initials: N
authors.lastname: Karnowski
authors.forename: Michael
authors.initials: M
authors.lastname: Famulok
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D004789:Enzyme Activation; D008163:Luminescent Measurements; D051379:Mice; D020559:Monomeric GTP-Binding Proteins / Q000378:metabolism*; D041681:NIH 3T3 Cells; D010100:Oxygen / Q000378:metabolism*; D013997:Time Factors
chemical_list: D020559:Monomeric GTP-Binding Proteins; D010100:Oxygen