title: In vitro and in vivo characterization of nonbiomedical- and biomedical-grade alginates for articular chondrocyte transplantation.
abstract: Alginate is a key hydrogel for cartilage tissue engineering. Here, we systematically evaluated four biomedical- and two nonbiomedical-grade alginates for their capacity to support the in vitro culture and in vivo transplantation of articular chondrocytes. Chondrocytes in all ultrapure alginates maintained high cell viability. Spheres composed of biomedical-grade, low-viscosity, high-mannuronic acid content alginate showed the lowest decrease in size over time. Biomedical-grade, low-viscosity, high-guluronic acid content alginate allowed for optimal cell proliferation. Biomedical-grade, medium-viscosity, high-mannuronic acid content alginate promoted the highest production of proteoglycans. When transplanted into osteochondral defects in the knee joint of sheep in vivo, empty spheres were progressively surrounded by a granulation tissue. In marked contrast with these observations, all alginate spheres carrying allogeneic chondrocytes were gradually invaded by a granulation tissue containing multinucleated giant cells, lymphocytes, and fibroblasts, regardless whether they were based on biomedical- or nonbiomedical-grade alginates. After 21 days in vivo, transplanted chondrocytes were either viable or underwent necrosis, and apoptosis played a minor role in their early fate. The individual characteristics of these alginates may be valuable to tailor specific experimental and clinical strategies for cartilage tissue engineering.
authors.affiliation: Experimental Orthopaedics and Osteoarthritis Research, Saarland University, Homburg, Germany.
authors.forename: Susanne
authors.initials: S
authors.lastname: Heiligenstein
authors.forename: Magali
authors.initials: M
authors.lastname: Cucchiarini
authors.forename: Matthias W
authors.initials: MW
authors.lastname: Laschke
authors.forename: Rainer M
authors.initials: RM
authors.lastname: Bohle
authors.forename: Dieter
authors.initials: D
authors.lastname: Kohn
authors.forename: Michael D
authors.initials: MD
authors.lastname: Menger
authors.forename: Henning
authors.initials: H
authors.lastname: Madry
mesh_terms: D000464:Alginates / Q000737:chemistry*; D017209:Apoptosis; D001672:Biocompatible Materials / Q000737:chemistry*; D002356:Cartilage / Q000637:transplantation; D002358:Cartilage, Articular / Q000166:cytology*; D002470:Cell Survival; D017690:Cell Transplantation / Q000379:methods*; D019902:Chondrocytes / Q000166:cytology*; D004731:Endotoxins / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D009336:Necrosis; D011509:Proteoglycans / Q000737:chemistry; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D000464:Alginates; D001672:Biocompatible Materials; D004731:Endotoxins; D006603:Hexuronic Acids; D011509:Proteoglycans; C008324:mannuronic acid