title: Recombinant canine granulocyte colony-stimulating factor accelerates recovery from cyclophosphamide-induced neutropenia in dogs.
abstract: In dogs injected intravenously with 400mg/m(2) cyclophosphamide (CPA), the peripheral neutrophil count decreased to less than 1000 cells/μL in 5-9 days. Treatment with purified recombinant canine granulocyte colony-stimulating factor (rcG-CSF), produced by brevibacillus expression system, at the nadir of the granulocyte count accelerated recovery from the CPA-induced neutropenia by 1-3 days. Therapeutic administration of rcG-CSF at doses of 2.5-10 μg/kg did not show any significant difference on the severity of neutropenia (the period that granulocyte counts were less than 2000 cells/μL). Administration of 2.5 μg/kg rcG-CSF 3 times per day 2-4 days or 3-5 days after CPA treatment not only accelerated recovery but also decreased the severity of neutropenia. No clinical signs of the rcG-CSF were observed. These results showed that the rcG-CSF is effective for treatment of neutropenia in dogs.
authors.affiliation: Nippon Institute for Biological Science, Shin-Machi 9-2221-1, Ome, Tokyo 198-0024, Japan. yamagen@nibs.or.jp
authors.forename: Akira
authors.initials: A
authors.lastname: Yamamoto
authors.forename: Miyuki
authors.initials: M
authors.lastname: Fujino
authors.forename: Takeshi
authors.initials: T
authors.lastname: Tsuchiya
authors.forename: Akira
authors.initials: A
authors.lastname: Iwata
mesh_terms: D000818:Animals; D003520:Cyclophosphamide / Q000009:adverse effects*; D004285:Dogs / Q000097:blood*; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D007958:Leukocyte Count / Q000662:veterinary; D009503:Neutropenia / Q000139:chemically induced* / Q000188:drug therapy*
chemical_list: D016179:Granulocyte Colony-Stimulating Factor; D003520:Cyclophosphamide