title: Expression of HGF and VEGF in the cerebral tissue of adult rats with chronic hydrocephalus after subarachnoid hemorrhage.
abstract: The hepatocyte growth factor (HGF) and vascular endothelial growth factor (VEGF) are important cytokines with modulatory actions in the nervous system. The present study aimed to investigate the role and expression of HGF and VEGF in the cerebral tissue of adult rats with chronic hydrocephalus after subarachnoid hemorrhage. Adult female Wistar rats were randomly divided into 4 groups: a control group (n=20) and 3 experimental subgroups (n=60). Subarachnoid hemorrhage was induced by the injection of 0.4 ml of non-heparinized autologous arterial blood into the cisterna magna of experimental animals on day 0 with a second injection 2 days later. The rats were sacrificed within 24 h of magnetic resonance imaging (MRI) examination at 2, 4, or 6 weeks. The excised brains were studied by RT-PCR, immunohistochemical and Western blot analyses as we examined HGF and VEGF mRNA and protein expression. Chronic hydrocephalus was induced in 21 rats after subarachnoid hemorrhage. After 2 weeks, the expression of HGF and VEGF in the cerebral tissue was significantly increased in the experimental group compared to the controls, especially in periventricular white matter. Our results indicate that HGF and VEGF participate in the pathological injury and repair of cerebral tissue in rats with chronic hydrocephalus after subarachnoid hemorrhage.
authors.affiliation: Department of Neurosurgery, No. 3 People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 201900, P.R. China. shenghuachu@126.com
authors.forename: Sheng-Hua
authors.initials: SH
authors.lastname: Chu
authors.forename: Dong-Fu
authors.initials: DF
authors.lastname: Feng
authors.forename: Yan-Bin
authors.initials: YB
authors.lastname: Ma
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.forename: Zhi-An
authors.initials: ZA
authors.lastname: Zhu
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Li
authors.forename: Zi-Heng
authors.initials: ZH
authors.lastname: Zhang
mesh_terms: D000375:Aging / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D015153:Blotting, Western; D002552:Cerebral Ventricles / Q000378:metabolism / Q000473:pathology; D054022:Cerebrum / Q000378:metabolism* / Q000473:pathology*; D002908:Chronic Disease; D003720:Densitometry; D005260:Female; D005786:Gene Expression Regulation; D017228:Hepatocyte Growth Factor / Q000235:genetics / Q000378:metabolism*; D006849:Hydrocephalus / Q000209:etiology* / Q000235:genetics; D007150:Immunohistochemistry; D008279:Magnetic Resonance Imaging; D009929:Organ Size; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013194:Staining and Labeling; D013345:Subarachnoid Hemorrhage / Q000150:complications* / Q000235:genetics; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; C467498:vascular endothelial growth factor A, rat; D017228:Hepatocyte Growth Factor