title: Molecular cloning and expression analysis of porcine ghrelin o-acyltransferase.
abstract: The peptide hormone ghrelin is secreted in the stomach, with unique N-octanoylation at serine 3, which is a requirement for its functionality. These functions include growth hormone release, appetite stimulation, gastrointestinal motility, glucose regulation, and cell proliferation. The enzyme responsible for ghrelin acylation was recently identified as ghrelin O-acyltransferase (GOAT). In this study, porcine GOAT was cloned and characterized. A full-length cDNA of GOAT of 2013 bp was obtained, which included a 70-bp 5' UTR, a 635-bp 3' UTR, and a 1308-bp open reading frame encoding a protein of 415 amino acids. The GOAT and ghrelin mRNAs are co-expressed in stomach, pancreas, and duodenum at high levels. GOAT was also detected in liver, lung, brain, testis, spleen, kidney, heart, muscle, lipid, and ovary. Our results provide an important basis for further research on GOAT function and the relationship between ghrelin and GOAT.
authors.affiliation: State Key Laboratory for Agrobiotechnology, China Agricultural University, Yuanmingyuan West Road No. 2, Haidian District, Beijing 100193, China.
authors.forename: Tonghui
authors.initials: T
authors.lastname: Lin
authors.forename: Qingyong
authors.initials: Q
authors.lastname: Meng
authors.forename: Dandan
authors.initials: D
authors.lastname: Sui
authors.forename: Dezhi
authors.initials: D
authors.lastname: Peng
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Liu
authors.forename: Longfei
authors.initials: L
authors.lastname: Xie
authors.forename: Ning
authors.initials: N
authors.lastname: Li
mesh_terms: D000217:Acyltransferases / Q000096:biosynthesis* / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D020134:Catalytic Domain; D003001:Cloning, Molecular; D005260:Female; D015870:Gene Expression; D008297:Male; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010802:Phylogeny; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D034421:Sus scrofa / Q000235:genetics*
chemical_list: D011994:Recombinant Proteins; D000217:Acyltransferases