title: TTF1-induced apoptosis of HepG-2 cells through a mitochondrial pathway.
abstract: The Chinese medicinal herb Sorbaria sorbifolia, native to Changbai Mountain, can induce apoptosis in HepG-2 cells. We studied the mechanism by which 5, 2', 4'-trihydroxy-6,7, 5'-trimethoxyflavone (TTF1) isolated from acetic ether extracts of Sorbaria sorbifolia induces apoptosis in HepG-2 cells. The results showed that TTF1 both inhibited cell growth and induced apoptosis. RT-PCR and Western blot analysis showed that TTF1 treatment led to decreased transcription and protein expression of bcl-2 and an increase in bax, Cyt-c, caspase-3 and caspase-9. These results together suggest that TTF1 may induce apoptosis of HepG-2 cells through a mitochondrial pathway.
authors.affiliation: Basic Medical College, Yanbian University, Yanji, Jilin 133000, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Lei
authors.initials: L
authors.lastname: Bian
authors.forename: Fengde
authors.initials: F
authors.lastname: Cui
authors.forename: Liangchang
authors.initials: L
authors.lastname: Li
authors.forename: Xuewu
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis; D020169:Caspases / Q000235:genetics / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D045304:Cytochromes c / Q000235:genetics / Q000378:metabolism; D053938:DNA Fragmentation; D004354:Drug Screening Assays, Antitumor; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D047309:Flavones / Q000302:isolation & purification / Q000494:pharmacology*; D015870:Gene Expression; D056945:Hep G2 Cells / Q000187:drug effects; D006801:Humans; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D027824:Rosaceae; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism
chemical_list: C494889:5,2',4'-trihydroxy-6,7,5'-trimethoxyflavone; D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D047309:Flavones; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D045304:Cytochromes c; D020169:Caspases