title: Effects of homeodomain protein CDX2 expression on the proliferation and migration of lovo colon cancer cells.
abstract: The homeobox gene, CDX2, plays a major role in development, especially in the gut, and also functions as a tumor suppressor in the adult colon. In the present study, we investigated the effects of CDX2 expression on the proliferation, migration, and apoptosis of the human colon cancer cell line, Lovo. Lovo cells exogenously expressing CDX2 exhibited no significant differences in the percentage of cells in G1- and S-phase or in apoptosis, as determined by flow cytometry. MTT assay also confirmed that CDX2 expression had no effect on proliferation in these cells. Interestingly, conditioned medium collected from CDX2-overexpressing Lovo cells showed a significant decrease in secretion of MMP-2 and the invasive potential of these cells was significantly inhibited. Collectively, these data suggest that CDX2 may play a critical role in the migration and metastasis of colon carcinoma and over-expression of CDX2 in colon cancer cells markedly inhibits invasion. Based on these results, exogenous expression of CDX2 might be a promising option in the treatment of colon carcinoma.
authors.affiliation: Department of General Surgery, First Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an, People's Republic of China.
authors.forename: Jian-bao
authors.initials: JB
authors.lastname: Zheng
authors.forename: Xue-jun
authors.initials: XJ
authors.lastname: Sun
authors.forename: Jie
authors.initials: J
authors.lastname: Qi
authors.forename: Shou-shuai
authors.initials: SS
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Hai-liang
authors.initials: HL
authors.lastname: Ren
authors.forename: Yong
authors.initials: Y
authors.lastname: Tian
authors.forename: Shao-ying
authors.initials: SY
authors.lastname: Lu
authors.forename: Jun-kai
authors.initials: JK
authors.lastname: Du
mesh_terms: D017209:Apoptosis; D015153:Blotting, Western; D000071616:CDX2 Transcription Factor; D002448:Cell Adhesion; D002465:Cell Movement; D049109:Cell Proliferation; D003110:Colonic Neoplasms / Q000378:metabolism* / Q000473:pathology*; D005434:Flow Cytometry; D016193:G1 Phase; D018398:Homeodomain Proteins / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D014407:Tumor Cells, Cultured
chemical_list: D000071616:CDX2 Transcription Factor; C491270:CDX2 protein, human; D018398:Homeodomain Proteins