title: A novel strontium-doped calcium polyphosphate/erythromycin/poly(vinyl alcohol) composite for bone tissue engineering.
abstract: It is our goal to develop bactericidal bone scaffolds with osteointegration potential. In this study, poly(vinyl alcohol) (PVA) coating (7%) was applied to an erythromycin (EM)-impregnated strontium-doped calcium polyphosphate (SCPP) scaffold using a simple slurry dipping method. MicroCT analysis showed that PVA coating reduced the average pore size and the percentage of pore interconnectivity to some extent. Compressive strength tests confirmed that the PVA coating significantly increased material elasticity and slightly enhanced the scaffold mechanical strength. It was also confirmed that the PVA coatings allowed a sustained EM release that is controlled by diffusion through the intact PVA hydrogel layer, irrespective of the drug solubility. PVA coating did not inhibit the EM bioactivity when the scaffolds were immersed in simulated body fluid for up to 4 weeks. EM released from SCPP-EM-PVA composite scaffolds maintained its capability of bacterial growth (S. aureus) inhibition. PVA coating is biocompatible and nontoxic to MC3T3 preosteoblast cells. Furthermore, we found that SCPP-EM-PVA composite scaffolds and their eluants remarkably inhibited RANKL-induced osteoclastogenesis in a murine RAW 264.7 macrophage cell line. Thus, this unique multifunctional bioactive composite scaffold has the potential to provide controlled delivery of relevant drugs for bone tissue engineering.
authors.affiliation: Department of Biomedical Engineering, Wayne State University, Detroit, Michigan 48201, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Song
authors.forename: Weiping
authors.initials: W
authors.lastname: Ren
authors.forename: Changxiu
authors.initials: C
authors.lastname: Wan
authors.forename: Amanda O
authors.initials: AO
authors.lastname: Esquivel
authors.forename: Tong
authors.initials: T
authors.lastname: Shi
authors.forename: Ralph
authors.initials: R
authors.lastname: Blasier
authors.forename: David C
authors.initials: DC
authors.lastname: Markel
mesh_terms: D016475:3T3 Cells; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D018786:Bone Substitutes / Q000737:chemistry* / Q000378:metabolism; D002118:Calcium / Q000737:chemistry* / Q000378:metabolism; D002454:Cell Differentiation; D002460:Cell Line; D019245:Compressive Strength; D004548:Elasticity; D004917:Erythromycin / Q000008:administration & dosage*; D008264:Macrophages / Q000166:cytology; D008422:Materials Testing; D051379:Mice; D010006:Osteoblasts / Q000166:cytology; D011122:Polyphosphates / Q000737:chemistry* / Q000378:metabolism; D011142:Polyvinyl Alcohol / Q000737:chemistry / Q000378:metabolism; D013324:Strontium / Q000737:chemistry* / Q000378:metabolism; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D018786:Bone Substitutes; D011122:Polyphosphates; D004917:Erythromycin; D011142:Polyvinyl Alcohol; D002118:Calcium; D013324:Strontium