title: Heterophil functional responses to dietary immunomodulators vary in genetically distinct chicken lines.
abstract: The effect of dietary supplementation of immunomodulators on in vitro chicken heterophil function was investigated using three diverse genetic lines of chickens (broiler, Fayoumi, and Leghorn). Dietary supplementation with β-glucan, ascorbic acid, and corticosterone was fed from 8 to 11 weeks of age. Heterophil function was evaluated weekly during supplementation using phagocytosis, bacterial killing, and heterophil extracellular traps (HETs)-DNA release. Fayoumis fed the basal diet had significantly higher HETs-DNA release (P=0.002) than Leghorns and broilers. Both genetic line and immunomodulator diet supplementation had significant effects on bacterial killing (line and diet effect: P<0.001) and HETs-DNA release (line: P<0.001; diet: P=0.043). Dietary supplementation with immunomodulators, therefore, shows potential to affect and augment heterophil function in chickens. The current results also suggest the important role of genetics in innate immune responses.
authors.affiliation: Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, United States.
authors.forename: Phongsakorn
authors.initials: P
authors.lastname: Chuammitri
authors.forename: Sarah B
authors.initials: SB
authors.lastname: Redmond
authors.forename: Kayoko
authors.initials: K
authors.lastname: Kimura
authors.forename: Claire B
authors.initials: CB
authors.lastname: Andreasen
authors.forename: Susan J
authors.initials: SJ
authors.lastname: Lamont
authors.forename: Dušan
authors.initials: D
authors.lastname: Palić
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000276:immunology; D002645:Chickens / Q000097:blood / Q000235:genetics / Q000276:immunology*; D003345:Corticosterone / Q000276:immunology; D019587:Dietary Supplements / Q000592:standards*; D007113:Immunity, Innate / Q000276:immunology*; D007155:Immunologic Factors / Q000276:immunology* / Q000494:pharmacology; D016018:Least-Squares Analysis; D010587:Phagocytosis / Q000276:immunology; D011897:Random Allocation; D012477:Salmonella enteritidis / Q000254:growth & development; D047071:beta-Glucans / Q000276:immunology
chemical_list: D007155:Immunologic Factors; D047071:beta-Glucans; D001205:Ascorbic Acid; D003345:Corticosterone