title: Measurement of circular dichroism dynamics in a nanosecond temperature-jump experiment.
abstract: The use of a fast temperature jump (T-jump) is a very powerful experiment aiming at studying protein denaturation dynamics. However, probing the secondary structure is a difficult challenge and rarely yields quantitative values. We present the technical implementation of far-UV circular dichroism in a nanosecond T-jump experiment and show that this experiment allows us to follow quantitatively the change in the helical fraction of a poly(glutamic acid) peptide during its thermal denaturation with 12 ns time resolution.
authors.affiliation: Laboratoire d'Optique et Biosciences, Ecole Polytechnique∕CNRS∕INSERM, 91128 Palaiseau, France.
authors.forename: Mai-Thu
authors.initials: MT
authors.lastname: Khuc
authors.forename: Lucille
authors.initials: L
authors.lastname: Mendonça
authors.forename: Sapna
authors.initials: S
authors.lastname: Sharma
authors.forename: Xavier
authors.initials: X
authors.lastname: Solinas
authors.forename: Martin
authors.initials: M
authors.lastname: Volk
authors.forename: François
authors.initials: F
authors.lastname: Hache
mesh_terms: D000042:Absorption; D002942:Circular Dichroism / Q000295:instrumentation / Q000379:methods*; D007834:Lasers; D011099:Polyglutamic Acid / Q000737:chemistry; D011489:Protein Denaturation; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature; D013997:Time Factors
chemical_list: D011099:Polyglutamic Acid