title: Conformational dynamics promote binding diversity of dynein light chain LC8.
abstract: A highly conserved and ubiquitous protein known as LC8 binds over twenty different partners, characteristic of a molecular hub (Barbar, 2008 Biochemistry, 47, 503-508). Structural studies of LC8 complexes with binding partners having diverse recognition sequences show that the same binding groove of LC8 accommodates the various binding motifs. Here we use thermodynamics and dynamics measurements of LC8 complexes to group LC8 binding partners in two categories: those whose binding is enthalpically driven and those that are entropically favored. Peptides that are enthalpically driven completely silence the millisecond-microsecond relaxation signal, suggesting a significant rigidifying of the binding groove, while peptides in the entropically favored group exhibit the same conformational dynamics as the free protein, suggesting that the peptide sits loosely in the binding groove and so retains flexibility of the groove, and presumably of the bound peptide. The inherent disorder in the LC8 binding groove and in LC8 binding partners allows both types of binding, accounts for the lack of a conserved recognition consensus motif and underlies the binding specificity and broad selectivity observed in LC8 binding.
authors.affiliation: Department of Biochemistry and Biophysics, Oregon State University, Corvallis, OR 97331, United States.
authors.forename: Afua
authors.initials: A
authors.lastname: Nyarko
authors.forename: Justin
authors.initials: J
authors.lastname: Hall
authors.forename: Andrea
authors.initials: A
authors.lastname: Hall
authors.forename: Michael
authors.initials: M
authors.lastname: Hare
authors.forename: Joachim
authors.initials: J
authors.lastname: Kremerskothen
authors.forename: Elisar
authors.initials: E
authors.lastname: Barbar
mesh_terms: D020816:Amino Acid Motifs; D056445:Cytoplasmic Dyneins / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation; D013816:Thermodynamics
chemical_list: D010455:Peptides; C427758:DYNLL1 protein, human; D056445:Cytoplasmic Dyneins