title: Fluorescence signal transduction mechanism for immunoassay based on zinc ion release from ZnS nanocrystals.
abstract: In this work, a fluorescence signal transduction mechanism based on cation release from ZnS nanocrystals was developed for sandwich immunoassay. In this mechanism, ZnS nanocrystals as labels in immunoassay are dissolved by acid to release zinc ions. After pH adjustment of the dissolving solution using a basic solution, zinc-ion sensitive fluorescence indicator Fluozin-3 is added to bind with the released zinc ions for sensitive fluorescence measurement. Using mouse IgG as a model analyte, the immunoassay adopting this signal transduction mechanism demonstrates a low detection limit around 1 pM and a detection range with two orders of magnitude (1 pM to 0.5 nM).
authors.affiliation: Department of Electrical and Biomedical Engineering, University of Nevada Reno, Reno, Nevada 89557-0260, USA.
authors.forename: Chad L
authors.initials: CL
authors.lastname: Cowles
authors.forename: Xiaoshan
authors.initials: X
authors.lastname: Zhu
authors.forename: Nelson G
authors.initials: NG
authors.lastname: Publicover
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D001710:Biotin / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007118:Immunoassay / Q000379:methods*; D007074:Immunoglobulin G / Q000737:chemistry / Q000276:immunology; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D051379:Mice; D011083:Polycyclic Compounds / Q000737:chemistry*; D019809:Streptavidin / Q000737:chemistry; D013440:Sulfides / Q000737:chemistry*; D015032:Zinc / Q000032:analysis*; D017967:Zinc Compounds / Q000737:chemistry*
chemical_list: D000906:Antibodies; C451182:FluoZin-3; D007074:Immunoglobulin G; D011083:Polycyclic Compounds; D013440:Sulfides; D017967:Zinc Compounds; D001710:Biotin; D019809:Streptavidin; D015032:Zinc; C031238:zinc sulfide