title: Imaging intracellular pH in live cells with a genetically encoded red fluorescent protein sensor.
abstract: Intracellular pH affects protein structure and function, and proton gradients underlie the function of organelles such as lysosomes and mitochondria. We engineered a genetically encoded pH sensor by mutagenesis of the red fluorescent protein mKeima, providing a new tool to image intracellular pH in live cells. This sensor, named pHRed, is the first ratiometric, single-protein red fluorescent sensor of pH. Fluorescence emission of pHRed peaks at 610 nm while exhibiting dual excitation peaks at 440 and 585 nm that can be used for ratiometric imaging. The intensity ratio responds with an apparent pK(a) of 6.6 and a >10-fold dynamic range. Furthermore, pHRed has a pH-responsive fluorescence lifetime that changes by ~0.4 ns over physiological pH values and can be monitored with single-wavelength two-photon excitation. After characterizing the sensor, we tested pHRed's ability to monitor intracellular pH by imaging energy-dependent changes in cytosolic and mitochondrial pH.
authors.affiliation: Department of Neurobiology, Harvard Medical School, Boston, Massachusetts 02115, USA.
authors.forename: Mathew
authors.initials: M
authors.lastname: Tantama
authors.forename: Yin Pun
authors.initials: YP
authors.lastname: Hung
authors.forename: Gary
authors.initials: G
authors.lastname: Yellen
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D005818:Genetic Engineering / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D042541:Intracellular Space / Q000737:chemistry* / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics*; D051379:Mice; D057054:Molecular Imaging / Q000379:methods*; D016296:Mutagenesis; D013050:Spectrometry, Fluorescence; D000097573:Red Fluorescent Protein
chemical_list: D008164:Luminescent Proteins; D000097573:Red Fluorescent Protein