title: Determination of the cyanobacterial toxin cylindrospermopsin in algal food supplements.
abstract: For the analysis of blue-green algal food supplements for cylindrospermopsin (CYN), a C18 solid-phase extraction column and a polygraphitized carbon solid-phase extraction column in series was an effective procedure for the clean-up of extracts. Determination of CYN was by liquid chromatography with ultraviolet light detection. At extract spiking levels of CYN equivalent to 25-500 µg g(-1), blue-green algal supplement recoveries were in the range 70-90%. CYN was not detected in ten samples of food supplements and one chocolate product, all containing blue-green algae. The limit of detection for the method was 16 µg g(-1), and the limit of quantification was 52 µg g(-1).
authors.affiliation: Bureau of Chemical Safety, Health Products and Food Branch, Health Canada, Ottawa, Ontario, Canada.
authors.forename: H
authors.initials: H
authors.lastname: Liu
authors.forename: P M
authors.initials: PM
authors.lastname: Scott
mesh_terms: D000470:Alkaloids; D001427:Bacterial Toxins / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D002099:Cacao / Q000737:chemistry; D002182:Candy / Q000032:analysis; D002273:Carcinogens / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D000458:Cyanobacteria / Q000378:metabolism*; D000087522:Cyanobacteria Toxins; D019587:Dietary Supplements / Q000032:analysis*; D057140:Fast Foods / Q000032:analysis; D005506:Food Contamination; D057230:Limit of Detection; D052616:Solid Phase Extraction; D013056:Spectrophotometry, Ultraviolet; D014498:Uracil / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D000470:Alkaloids; D001427:Bacterial Toxins; D002273:Carcinogens; D000087522:Cyanobacteria Toxins; C089595:cylindrospermopsin; D014498:Uracil