title: Genomes of three methylotrophs from a single niche reveal the genetic and metabolic divergence of the methylophilaceae.
abstract: The genomes of three representatives of the family Methylophilaceae, Methylotenera mobilis JLW8, Methylotenera versatilis 301, and Methylovorus glucosetrophus SIP3-4, all isolated from a single study site, Lake Washington in Seattle, WA, were completely sequenced. These were compared to each other and to the previously published genomes of Methylobacillus flagellatus KT and an unclassified Methylophilales strain, HTCC2181. Comparative analysis revealed that the core genome of Methylophilaceae may be as small as approximately 600 genes, while the pangenome may be as large as approximately 6,000 genes. Significant divergence between the genomes in terms of both gene content and gene and protein conservation was uncovered, including the varied presence of certain genes involved in methylotrophy. Overall, our data demonstrate that metabolic potentials can vary significantly between different species of Methylophilaceae, including organisms inhabiting the very same environment. These data suggest that genetic divergence among the members of this family may be responsible for their specialized and nonredundant functions in C₁ cycling, which in turn suggests means for their successful coexistence in their specific ecological niches.
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors.forename: Alla
authors.initials: A
authors.lastname: Lapidus
authors.forename: Alicia
authors.initials: A
authors.lastname: Clum
authors.forename: Kurt
authors.initials: K
authors.lastname: Labutti
authors.forename: Marina G
authors.initials: MG
authors.lastname: Kaluzhnaya
authors.forename: Sujung
authors.initials: S
authors.lastname: Lim
authors.forename: David A C
authors.initials: DA
authors.lastname: Beck
authors.forename: Tijana
authors.initials: T
authors.lastname: Glavina Del Rio
authors.forename: Matt
authors.initials: M
authors.lastname: Nolan
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Mavromatis
authors.forename: Marcel
authors.initials: M
authors.lastname: Huntemann
authors.forename: Susan
authors.initials: S
authors.lastname: Lucas
authors.forename: Mary E
authors.initials: ME
authors.lastname: Lidstrom
authors.forename: Natalia
authors.initials: N
authors.lastname: Ivanova
authors.forename: Ludmila
authors.initials: L
authors.lastname: Chistoserdova
mesh_terms: D019143:Evolution, Molecular; D005618:Fresh Water / Q000382:microbiology; D016680:Genome, Bacterial; D000432:Methanol / Q000378:metabolism; D042762:Methylophilaceae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data
chemical_list: D000432:Methanol