title: IFN-γ plays an essential role in the pathogenesis of gastric lymphoid follicles formation caused by Helicobacter suis infection.
abstract: In this study, we aimed to assess the role of helper T cells in the development of gastric lymphoid follicles induced by Helicobacter suis infection. C57BL/6J mice were orally inoculated with H. suis. Six weeks after infection, gastric lymphoid follicles were observed in the gastric mucosa by hematoxylin and eosin staining, and the number of follicles was increased throughout the infection period. An immunohistological examination showed that the lymphoid follicles were composed of B cells, CD4-positive helper T cells, and dendritic cells (DC). It was also revealed that the mRNA expression level of interferon-γ (IFN-γ) in the gastric mucosa was significantly increased at 12 weeks after infection. No gastric lymphoid follicles were detected in IFN-γ-deficient mice that had been infected with H. suis at 12 weeks after infection, although the development of lymphoid follicles in IL-4-deficient mice infected with H. suis was similar to that seen in the wild-type mice. In conclusion, IFN-γ, a Th1 cytokine, is deeply involved in the pathogenesis of gastric lymphoid follicles induced by H. suis infection, and it is suggested that CD4-positive T cells and DC aid in the expansion of gastric lymphoid follicles.
authors.affiliation: Department of Internal Medicine, Division of Gastroenterology, Kobe University Graduate School of Medicine, Kobe, Hyogo, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Mimura
authors.forename: Masaru
authors.initials: M
authors.lastname: Yoshida
authors.forename: Shin
authors.initials: S
authors.lastname: Nishiumi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tanaka
authors.forename: Kentaro
authors.initials: K
authors.lastname: Nobutani
authors.forename: Mamoru
authors.initials: M
authors.lastname: Takenaka
authors.forename: Yahaya Ben
authors.initials: YB
authors.lastname: Suleiman
authors.forename: Koji
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Ota
authors.forename: Shinichi
authors.initials: S
authors.lastname: Takahashi
authors.forename: Hidenori
authors.initials: H
authors.lastname: Matsui
authors.forename: Masahiko
authors.initials: M
authors.lastname: Nakamura
authors.forename: Ikuya
authors.initials: I
authors.lastname: Miki
authors.forename: Takeshi
authors.initials: T
authors.lastname: Azuma
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology; D005753:Gastric Mucosa / Q000276:immunology / Q000382:microbiology* / Q000473:pathology*; D020869:Gene Expression Profiling; D018247:Helicobacter heilmannii / Q000276:immunology* / Q000472:pathogenicity*; D006651:Histocytochemistry; D007150:Immunohistochemistry; D007371:Interferon-gamma / Q000096:biosynthesis / Q000172:deficiency / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008853:Microscopy; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D013997:Time Factors
chemical_list: D012333:RNA, Messenger; D007371:Interferon-gamma