title: Overexpression of thioredoxin system proteins predicts poor prognosis in patients with squamous cell carcinoma of the tongue.
abstract: The human thioredoxin (Trx) system plays a critical role in the regulation of cellular reduction-oxidation (redox) homeostasis, which has been widely investigated in several types of cancer because of its association with cell growth and anti-apoptosis progress. This study aimed to evaluate the expression of Trx and Trx reductase-1 (TrxR-1) and explore the potential role of these proteins in tongue squamous cell carcinoma (TSCC). Immunohistochemistry was employed to analyze the protein expression levels of Trx and TrxR-1 in 65 TSCC tissue samples and 10 normal oral mucosa samples. The results were then evaluated semiquantitatively and compared to other clinicopathological variables. Both Trx and TrxR-1 expression levels were significantly higher in TSCC tissues as compared with the 10 normal oral mucous samples (P<0.01). A highly significant association between Trx and TrxR-1 expression in TSCCs was revealed (P=0.001), and the expression of Trx was correlated with tumour cell differentiation (P=0.001). Moreover, Kaplan-Meier analysis revealed that Trx expression and TNM stage were significantly related with 5-year survival rate (P=0.033, 0.000), while TrxR-1 expression was not associated with survival (P=0.092). The results indicated that high expression of Trx and TrxR-1 was associated with tumourigenesis in TSCC, and overexpression of Trx might predict poor prognosis.
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST), School & Hospital of Stomatology, Wuhan University, 237 Luoyu Road, Wuhan 430079, People's Republic of China.
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Zhu
authors.forename: Congfa
authors.initials: C
authors.lastname: Huang
authors.forename: Bin
authors.initials: B
authors.lastname: Peng
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000401:mortality; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000378:metabolism*; D011237:Predictive Value of Tests; D011379:Prognosis; D054481:Thioredoxin Reductase 1 / Q000378:metabolism*; D013879:Thioredoxins / Q000378:metabolism*; D014062:Tongue Neoplasms / Q000378:metabolism* / Q000401:mortality; D014407:Tumor Cells, Cultured
chemical_list: D009363:Neoplasm Proteins; D013879:Thioredoxins; D054481:Thioredoxin Reductase 1