title: [Latent membrane protein-1 of EB virus and the phenotype of epithelial-mesenchymal transition and cervical lymph node metastasis in nasopharyngeal carcinoma].
abstract: OBJECTIVE
  To evaluate the relation of EB virus latent membrane protein-1 (LMP-1) and the phenotype of epithelial-mesenchymal transition (EMT) and cervical lymph node metastasis in nasopharyngeal carcinoma.
  
  
  METHODS
  Based on histopathology and MRI imaging, nasopharyngeal biopsy tissues from 88 patients with nasopharyngeal carcinoma were divided into 3 groups: pathologic metastasis (18), MRI metastasis(40) and without metastasis (30). The expressions of LMP-1, STAT3, Twist, E-Cadherin and Vimentin were examined immunohistochemically in biopsy tissues.
  
  
  RESULTS
  LMP-1 expression was found in 35 of 88 biopsy tissues with a positive rate of 38.7%. The positive rates of LMP-1 in groups of pathologic metastasis, MRI metastasis and without metastasis were 38.9% (7/18), 47.5% (19/40) and 30.0% (9/30), respectively, and significant difference were not found among three groups. The expression of LMP-1 was positively correlated to both expressions of Twist and Vimentin (r = 0.276 and 0.282, are P < 0.01), but not to both expressions of STAT3 and E-Cadherin. The positive expressions or abnormal expression of STAT3, Twist, Vimentin and E-Cadherin were found in 57 of 88 (64.8%), 48 of 88 (54.5%), 22 of 88 (20.0%)and 53 of 88 (60.2%), respectively. Significant differences in the expression of STAT3, Twist, Vimentin and E-Cadherin were all found among groups of pathologic metastasis, MRI metastasis and without metastasis, respectively (are P < 0.05). The expression of STAT3 was positively correlated to both expressions of Twist and Vimentin (r = 0.712 and 0.316, P < 0.01).
  
  
  CONCLUSIONS
  EMT plays important role in cervical lymph node metastasis in nasopharyngeal carcinoma. LMP-1 may be only as one of upstream factors associated with the EMT, but not the decisive factor for cervical lymph node metastasis.
authors.affiliation: Department of Otorhinolaryngology, the First Affiliated Hospital of Jiaxing College, Jiaxing, 314000, China. yuelihuazj@hotmail.com
authors.forename: Lihua
authors.initials: L
authors.lastname: Yue
authors.forename: Zhiyi
authors.initials: Z
authors.lastname: Jiang
authors.forename: Wanxin
authors.initials: W
authors.lastname: Wu
authors.forename: Yanping
authors.initials: Y
authors.lastname: Zhang
authors.forename: Peng
authors.initials: P
authors.lastname: Yin
authors.forename: Yajun
authors.initials: Y
authors.lastname: Zhang
authors.forename: Cheng
authors.initials: C
authors.lastname: Sheng
authors.forename: Guobiao
authors.initials: G
authors.lastname: Wei
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
authors.forename: Kai
authors.initials: K
authors.lastname: Ling
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015820:Cadherins / Q000378:metabolism; D058750:Epithelial-Mesenchymal Transition; D005260:Female; D004854:Herpesvirus 4, Human / Q000378:metabolism*; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009303:Nasopharyngeal Neoplasms / Q000473:pathology* / Q000821:virology*; D009333:Neck / Q000473:pathology; D009687:Nuclear Proteins / Q000378:metabolism; D050796:STAT3 Transcription Factor / Q000378:metabolism; D051879:Twist-Related Protein 1 / Q000378:metabolism; D014746:Vimentin / Q000378:metabolism; D014763:Viral Matrix Proteins / Q000378:metabolism*; D055815:Young Adult
chemical_list: D015820:Cadherins; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D009687:Nuclear Proteins; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; C103173:TWIST1 protein, human; D051879:Twist-Related Protein 1; D014746:Vimentin; D014763:Viral Matrix Proteins