title: Molecular cloning and its expression of trachealess gene (As-trh) during development in brine shrimp, Artemia sinica.
abstract: Basic helix-loop-helix-PAS (bHLH-PAS) family transcription factors are implicated in multiple developmental and physiological regulatory processes. Herein, a full-length cDNA encoding a bHLH-PAS domain transcription factor trachealess gene (designated as As-trh) was cloned and characterized from brine shrimp (Artemia sinica) for the first time. The full-length cDNA of As-trh was 2,698 bp with a 2,319 bp open reading frame encoding a deduced protein of 772 amino acid polypeptide with a calculated molecular mass of 86.02 kDa and an isoelectric point of 5.87. Sequence alignment revealed that As-trh had high homology with other species trh gene, including the D-trh gene in Drosophila melanogaster and Bm-trh in Bombyx mori. The early and persistent expression of As-trh in the naupliar stages by whole-mount embryonic in situ hybridization and immunohistochemistry suggest that As-trh functions very early in the salt gland and may be required continuously in this tissue. Later in development, expression of As-trh begins to decrease and disappear in salt gland of the older nauplius and appears in the thoracic epipods of the sub-adult Artemia. These results indicated that As-trh might play an important role in osmoregulatiory organ development from the larvae stages through adult stages.
authors.affiliation: College of Ocean, Agricultural University of Hebei, Qinhuangdao 066003, People's Republic of China.
authors.forename: Jia-Qing
authors.initials: JQ
authors.lastname: Wang
authors.forename: Lin
authors.initials: L
authors.lastname: Hou
authors.forename: Nan
authors.initials: N
authors.lastname: Yi
authors.forename: Riu-Feng
authors.initials: RF
authors.lastname: Zhang
authors.forename: Xiang-Yang
authors.initials: XY
authors.lastname: Zou
authors.forename: Qin
authors.initials: Q
authors.lastname: Xiao
authors.forename: Ran
authors.initials: R
authors.lastname: Guo
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001156:Artemia / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D001483:Base Sequence; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D019295:Computational Biology; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010766:Phosphorylation; D017422:Sequence Analysis, DNA
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D017931:DNA Primers; D018076:DNA, Complementary