title: Polymorphisms of diacylglycerol acyltransferase 2 gene and their relationship with growth traits in goats.
abstract: Diacylglycerol acyltransferase (DGAT) plays a critical role in the synthesis of triacylglycerol. In this study, PCR-SSCP and DNA sequencing methods were employed to screen the genetic variations of DGAT-2 gene in 299 goats from three breeds (Boer goat, Chinese Xuhuai white goat and Chinese Haimen goat). Three fragments of DGAT-2 gene were investigated, only exon 3 of DGAT-2 gene showed polymorphism. The alignment between nucleotide sequences of NM_205793.2 in GenBank and the sequencing results of three PCR products with different patterns revealed that there was one mutation (A → G) in exon 3 of DGAT-2 gene, which resulted in amino acid change (Lys → Arg) and constructed two genotypes (AA, AB). The frequencies of allele A and genotype AA were dominant in all three breeds. And there was no significant difference for genotypic and allelic frequencies among the three breeds. The genotype distributions were in good agreement with Hardy-Weinberg equilibrium (P > 0.05) in each breed. Significant statistical differences were only found in withers heights (P < 0.05) in Xuhuai goat between genotypes. The results indicated that individuals with genotype AA were significantly higher than those of individuals with genotype AB in withers height (P < 0.05). No polymorphism was detected in the intron 3, exon 8 and 3' flanking region. So we suggested that DGAT-2 gene had the close relationship with growth traits in goats. And this mutation could be used as a perfect molecular marker for marker-assisted selection (MAS) in animal genetics and breeding.
authors.affiliation: College of Life Science, Xuzhou Normal University, Xuzhou, 221116 Jiangsu, China. xtfang@163.com
authors.forename: Xingtang
authors.initials: X
authors.lastname: Fang
authors.forename: Jingmin
authors.initials: J
authors.lastname: Zhang
authors.forename: Haixia
authors.initials: H
authors.lastname: Xu
authors.forename: Chunlei
authors.initials: C
authors.lastname: Zhang
authors.forename: Yu
authors.initials: Y
authors.lastname: Du
authors.forename: Xiuying
authors.initials: X
authors.lastname: Shi
authors.forename: Danxia
authors.initials: D
authors.lastname: Chen
authors.forename: Jiajie
authors.initials: J
authors.lastname: Sun
authors.forename: Qijiang
authors.initials: Q
authors.lastname: Jin
authors.forename: Xianyong
authors.initials: X
authors.lastname: Lan
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
mesh_terms: D000818:Animals; D001483:Base Sequence; D001837:Body Weights and Measures; D001947:Breeding / Q000379:methods; D051048:Diacylglycerol O-Acyltransferase / Q000235:genetics*; D005787:Gene Frequency; D005819:Genetic Markers / Q000235:genetics; D005838:Genotype; D006041:Goats / Q000033:anatomy & histology / Q000235:genetics* / Q000254:growth & development*; D008969:Molecular Sequence Data; D020125:Mutation, Missense / Q000235:genetics; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D018807:Polymorphism, Single-Stranded Conformational; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D005819:Genetic Markers; D051048:Diacylglycerol O-Acyltransferase