title: [The structure of the complexes of DNA with chromosomal protein HMGB1 and histone H1 in the presence of manganese ions. I. Circular dicroism spectroscopy].
abstract: The mechanisms of interaction of the non-histone chromosomal protein HMGB1 and linker histone H1 with DNA have been studied using circular dichroism and absorption spectroscopy. Both of the proteins are located in the inter-nucleosomal regions of chromatin. It was demonstrated that properties of the DNA-protein complexes depend on the protein content and can not be considered as a simple summing up of the effects of individual protein components. Interaction of HMGB1 and H1 proteins is shown to be co-operative rather than competitive. Lysine-rich histone H1 facilitates the binding of the HMGB1 with DNA by screening the negatively charged groups of the sugar-phosphate backbone of DNA and dicarboxylic amino-acid residues in the C-terminal domain of the HMGB1 protein. The observed joint action of the and H1 proteins stimulates DNA condensation with formation of the anisotropic DNA-protein complexes with typical psi-type CD spectra. Structural organization of the complexes depends not only on the DNA-protein interactions, but also on the interaction between HMGB1 and H1 protein molecules bound to DNA. Manganese ions significantly modify the character of interactions between the components in the triple DNA-HMGB1-H1 complex. Binding of Mn2+ ions causes the weakening of the DNA-protein interactions and strengthening the protein-protein interactions, which promote DNA condensation and formation of large DNA-protein particles in solution.
authors.forename: E V
authors.initials: EV
authors.lastname: Chikhirzhina
authors.forename: A M
authors.initials: AM
authors.lastname: Polianichko
authors.forename: E I
authors.initials: EI
authors.lastname: Kostyleva
authors.forename: V I
authors.initials: VI
authors.lastname: Vorob'ev
mesh_terms: D000818:Animals; D002417:Cattle; D002843:Chromatin / Q000737:chemistry*; D002942:Circular Dichroism; D024243:HMGB1 Protein / Q000737:chemistry*; D006657:Histones / Q000737:chemistry*; D008345:Manganese / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D009707:Nucleosomes / Q000737:chemistry*
chemical_list: D002843:Chromatin; D024243:HMGB1 Protein; D006657:Histones; D009707:Nucleosomes; D008345:Manganese