title: Detection and characterization of a ST6 clone of vanB2-Enterococcus faecalis from three different hospitals in Spain.
abstract: Thirteen vancomycin-resistant and teicoplanin-susceptible Enterococcus faecalis isolates were recovered from unrelated patients in three Spanish hospitals from November 2009 to December 2010. All isolates carried the vanB2 gene, showed indistinguishable or closely-related PFGE patterns and were ascribed to the sequence type ST6 (included into the high-risk clonal-complex CC2). They showed a multiresistance phenotype (erythromycin, tetracycline, ciprofloxacin and high-level-resistance to streptomycin, gentamicin and kanamycin) and harboured the aac(6')-aph(2"), ant(6)-Ia, and tet(M)+/-tet(L) genes. All isolates produced gelatinase and harboured the gelE gene, but not the esp or hyl genes. The inclusion of the vanB2 gene into the Tn5382 transposon was demonstrated in one isolate. Clonal dissemination of vanB2-containing the E. faecalis strain is demonstrated.
authors.affiliation: Área Bioquímica y Biología Molecular, Universidad de La Rioja, Madre de Dios 51, 26006, Logroño, Spain.
authors.forename: M
authors.initials: M
authors.lastname: López
authors.forename: A
authors.initials: A
authors.lastname: Rezusta
authors.forename: C
authors.initials: C
authors.lastname: Seral
authors.forename: C
authors.initials: C
authors.lastname: Aspiroz
authors.forename: C
authors.initials: C
authors.lastname: Marne
authors.forename: M J
authors.initials: MJ
authors.lastname: Aldea
authors.forename: I
authors.initials: I
authors.lastname: Ferrer
authors.forename: M J
authors.initials: MJ
authors.lastname: Revillo
authors.forename: F J
authors.initials: FJ
authors.lastname: Castillo
authors.forename: C
authors.initials: C
authors.lastname: Torres
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001426:Bacterial Proteins / Q000235:genetics*; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D013293:Enterococcus faecalis / Q000235:genetics* / Q000302:isolation & purification; D005260:Female; D018093:Gelatinases / Q000378:metabolism; D016908:Gram-Positive Bacterial Infections / Q000453:epidemiology / Q000235:genetics / Q000382:microbiology; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D013030:Spain / Q000453:epidemiology; D014552:Urinary Tract Infections / Q000382:microbiology; D020713:Vancomycin Resistance / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; C079913:VanB protein, Enterococcus; D018093:Gelatinases