title: Severe nutritional iron-deficiency anaemia has a negative effect on some bone turnover biomarkers in rats.
abstract: BACKGROUND
  The role of iron (Fe) in bone formation and disease have not received much attention, a fact that is interesting given the known biochemical role that this mineral has upon collagen maturation together with the high prevalence of Fe-deficiency anaemia worldwide.
  
  
  OBJECTIVE
  To investigate the changes in bone formation, resorption and mineral content in developing rats with induced nutritional Fe-deficiency anaemia.
  
  
  METHODS
  Thirty male Wistar rats were divided into two groups, a control group receiving AIN-93G diet with normal-Fe content and an anaemic group receiving AIN-93G diet with low-Fe content for 40 days. Both diets were prepared with an adequate calcium (Ca) and phosphorus (P) content. The most representative serum bone turnover biomarkers and femur and sternum calcium and phosphorus content, together with sternum Fe content were determined in both experimental groups.
  
  
  RESULTS
  In anaemic rats, bone matrix formation diminished as revealed by the lower amount of procollagen type I N-terminal propeptide. Bone resorption process increased in Fe deficiency as shown by the increase of serum parathyroid hormone, tartrate-resistant acid phosphatase and levels of degradation products from C-terminal telopeptides of type I collagen released to the serum. In addition, mineralization process was affected by Fe deficiency, because Ca and P content in femur decreased markedly.
  
  
  CONCLUSIONS
  Fe-deficiency anaemia had a significant impact upon bone, affecting bone mineralization, decreasing the matrix formation and increasing bone resorption, therefore it is of great interest to assess bone status in situation of Fe-deficiency anaemia.
authors.affiliation: Department of Physiology, Faculty of Pharmacy and Institute of Nutrition and Food Technology José Mataix, University of Granada, Campus Universitario de Cartuja, Granada, Spain. javierdc@ugr.es
authors.forename: Javier
authors.initials: J
authors.lastname: Díaz-Castro
authors.forename: Mercedes Ramírez
authors.initials: MR
authors.lastname: López-Frías
authors.forename: Margarita S
authors.initials: MS
authors.lastname: Campos
authors.forename: Magdalena
authors.initials: M
authors.lastname: López-Frías
authors.forename: María J M
authors.initials: MJ
authors.lastname: Alférez
authors.forename: Teresa
authors.initials: T
authors.lastname: Nestares
authors.forename: María L
authors.initials: ML
authors.lastname: Ojeda
authors.forename: Inmaculada
authors.initials: I
authors.lastname: López-Aliaga
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D018798:Anemia, Iron-Deficiency / Q000097:blood* / Q000150:complications; D000818:Animals; D015415:Biomarkers / Q000097:blood; D001842:Bone and Bones / Q000378:metabolism* / Q000503:physiopathology; D002113:Calcification, Physiologic / Q000187:drug effects; D002136:Calcium, Dietary / Q000097:blood; D004032:Diet; D005269:Femur / Q000378:metabolism; D005293:Ferritins / Q000097:blood; D019266:Iron, Dietary / Q000008:administration & dosage* / Q000097:blood; D007527:Isoenzymes / Q000378:metabolism; D008297:Male; D044342:Malnutrition / Q000097:blood* / Q000150:complications; D010281:Parathyroid Hormone / Q000378:metabolism; D010758:Phosphorus / Q000097:blood; D051381:Rats; D017208:Rats, Wistar; D000071681:Tartrate-Resistant Acid Phosphatase; D014807:Vitamin D / Q000031:analogs & derivatives / Q000097:blood
chemical_list: D015415:Biomarkers; D002136:Calcium, Dietary; D019266:Iron, Dietary; D007527:Isoenzymes; D010281:Parathyroid Hormone; D014807:Vitamin D; D010758:Phosphorus; D005293:Ferritins; C104450:25-hydroxyvitamin D; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase