title: Association of variable number of tandem repeats polymorphism in the IL-4 gene with end-stage renal disease in Malaysian patients.
abstract: Variable number of tandem repeats (VNTR) polymorphism in the interleukin 4 (IL-4) gene has been associated with end-stage renal disease (ESRD) subjects in many different populations, although with conflicting results. We determined the 70 bp of VNTR polymorphism at intron 3 of the IL-4 gene in Malaysian ESRD subjects. Buccal cells were collected from 160 case and 160 control subjects; genomic DNA was amplified using PCR, followed by agarose gel electrophoresis. There were significant differences in genotypes and alleles of the IL-4 gene. We conclude that VNTR polymorphism of the IL-4 gene is a risk factor for the development of ESRD among Malaysians.
authors.affiliation: Genetic Research Group, Molecular Biology and Bioinformatics Unit, Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Selangor, Malaysia.
authors.forename: R
authors.initials: R
authors.lastname: Vasudevan
authors.forename: M N
authors.initials: MN
authors.lastname: Norhasniza
authors.forename: I
authors.initials: I
authors.lastname: Patimah
mesh_terms: D006801:Humans; D015847:Interleukin-4 / Q000235:genetics*; D007676:Kidney Failure, Chronic / Q000235:genetics*; D008296:Malaysia; D018598:Minisatellite Repeats; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
chemical_list: D015847:Interleukin-4