title: Poly[N-(2-hydroxypropyl)methacrylamide]-based tissue-embedding medium compatible with MALDI mass spectrometry imaging experiments.
abstract: Traditional tissue-sectioning techniques for histological samples utilize various embedding media to stabilize the tissue on a sectioning target and to provide a smooth cutting surface. Due to the ion suppression effect in MALDI ionization and number of background peaks in the low-mass region, these media are not suitable for mass spectrometry imaging (MSI) experiments. To overcome this, droplets of water are often used to mount the tissue on a sectioning target, but the ice block formed around the tissue does not provide a good support for sectioning of fragile samples. In this work, we propose a novel embedding media, compatible with MALDI ionization and MSI experiments, based on poly[N-(2-hydroxypropyl)methacrylamide] (pHPMA). Using a reversible addition-fragmentation chain transfer polymerization technique, well-defined pHPMA polymer with narrow mass distribution was prepared. Benefits of the resulted pHPMA-based embedding media were tested on different tissue samples.
authors.affiliation: Institute of Microbiology AS CR, v.v.i., Prague, Czech Republic. strohalm@biomed.cas.cz
authors.forename: Martin
authors.initials: M
authors.lastname: Strohalm
authors.forename: Jiří
authors.initials: J
authors.lastname: Strohalm
authors.forename: Filip
authors.initials: F
authors.lastname: Kaftan
authors.forename: Lukáš
authors.initials: L
authors.lastname: Krásný
authors.forename: Michael
authors.initials: M
authors.lastname: Volný
authors.forename: Petr
authors.initials: P
authors.lastname: Novák
authors.forename: Karel
authors.initials: K
authors.lastname: Ulbrich
authors.forename: Vladimír
authors.initials: V
authors.lastname: Havlíček
mesh_terms: D000178:Acrylamides / Q000737:chemistry*; D000818:Animals; D001516:Bees; D008168:Lung; D051379:Mice; D011108:Polymers / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D016610:Tissue Embedding
chemical_list: D000178:Acrylamides; D011108:Polymers; C032976:N-(2-hydroxypropyl)methacrylamide