title: Experimental food allergy leads to adipose tissue inflammation, systemic metabolic alterations and weight loss in mice.
abstract: To investigate the consequences of food allergy in adipose tissue and metabolism, we used a murine model in which mice have been sensitized subcutaneously with ovalbumin and further received antigen-containing diet. Allergic mice presented a significant weight loss 7 days after oral challenge with a concomitant decrease in epididymal adipose tissue mass. This decrease was associated with increased lipolysis and local inflammation. In adipose tissue of allergic mice there were increased leukocyte rolling and adhesion in the microvasculature, increased number of leukocytes in the tissue, especially macrophages (F4/80(+) cells) and increased pro-inflammatory cytokines levels, including TNF-α, IL-6 and CCL2. In addition, we observed low serum concentrations of triglyceride, glucose, total cholesterol and free fatty acids in the allergic mice. Our results suggest that the induction of food allergy in mice leads to adipose tissue inflammation and systemic metabolic alterations that contribute to the weight loss observed.
authors.affiliation: Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Luana Pereira Antunes
authors.initials: LP
authors.lastname: Dourado
authors.forename: Maria de Lourdes Meirelles
authors.initials: Mde L
authors.lastname: Noviello
authors.forename: Débora Moreira
authors.initials: DM
authors.lastname: Alvarenga
authors.forename: Zélia
authors.initials: Z
authors.lastname: Menezes
authors.forename: Denise Alves
authors.initials: DA
authors.lastname: Perez
authors.forename: Nathália Vieira
authors.initials: NV
authors.lastname: Batista
authors.forename: Gustavo Batista
authors.initials: GB
authors.lastname: Menezes
authors.forename: Adaliene Versiani Matos
authors.initials: AV
authors.lastname: Ferreira
authors.forename: Danielle da Glória
authors.initials: Dda G
authors.lastname: de Souza
authors.forename: Denise Carmona
authors.initials: DC
authors.lastname: Cara
mesh_terms: D000273:Adipose Tissue / Q000276:immunology / Q000473:pathology*; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D002448:Cell Adhesion; D018925:Chemokines / Q000378:metabolism; D002784:Cholesterol / Q000097:blood; D016207:Cytokines / Q000378:metabolism; D004822:Epididymis / Q000276:immunology / Q000473:pathology; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005512:Food Hypersensitivity / Q000276:immunology / Q000378:metabolism* / Q000473:pathology*; D007249:Inflammation / Q000209:etiology / Q000473:pathology; D036904:Leukocyte Rolling; D008066:Lipolysis; D008264:Macrophages / Q000473:pathology; D008297:Male; D008407:Mast Cells / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology; D014280:Triglycerides / Q000097:blood; D015431:Weight Loss
chemical_list: D001786:Blood Glucose; D018925:Chemokines; D016207:Cytokines; D005230:Fatty Acids, Nonesterified; D014280:Triglycerides; D010047:Ovalbumin; D002784:Cholesterol