title: Cloning, mapping and molecular characterization of porcine progesterone receptor membrane component 2 (PGRMC2) gene.
abstract: Progesterone plays an important role in sow reproduction by stimulating classic genomic pathways via nuclear receptors and non-genomic pathways via membrane receptors such a progesterone receptor membrane component 2 (PGRMC2). In this work, we used radiation hybrid mapping to assign PGRMC2 to pig chromosome 8 and observed that this receptor has two transcripts in pigs. The full-length cDNA of the large transcript is 1858 bp long and contains a 669-bp open reading frame (ORF) encoding a protein of 223 amino acids. The shorter transcript encodes a protein of 170 amino acids. The porcine PGRMC2 gene consists of three exons 446 bp, 156 bp and 1259 bp in length. The promoter sequence is GC-rich and lacks a typical TATA box. Several putative cis-regulatory DNA motifs were identified in the 208-bp upstream genomic region. Five single nucleotide polymorphisms (SNPs) were detected in introns* and the 3' UTR. RT-PCR indicated that the PGRMC2 gene is expressed ubiquitously in all pig tissues examined.
authors.affiliation: Key Laboratory for Animal Biotechnology of Jiangxi Province and The Ministry of Agriculture of China, Jiangxi Agricultural University, Nanchang, Jiangxi Province People's Republic of China.
authors.forename: Congying
authors.initials: C
authors.lastname: Chen
authors.forename: Carole
authors.initials: C
authors.lastname: Sargent
authors.forename: Claire
authors.initials: C
authors.lastname: Quilter
authors.forename: Zhuqing
authors.initials: Z
authors.lastname: Yang
authors.forename: Jun
authors.initials: J
authors.lastname: Ren
authors.forename: Nabeel
authors.initials: N
authors.lastname: Affara
authors.forename: Bertram
authors.initials: B
authors.lastname: Brenig
authors.forename: Lusheng
authors.initials: L
authors.lastname: Huang