title: Simple, rapid and sensitive detection of antibiotics based on the side-by-side assembly of gold nanorod probes.
abstract: In this work, we report on the application of versatile gold nanorods (GNRs) in optical sensors for the detection of antibiotics. The target analyte, Gentamicin (GM) and ovalbumin (OVA)-antigen-modified GNRs together competed with antibody-modified GNRs, then influenced the formation of side-by-side aggregates of the GNRs by antibody-antigen interactions. Accordingly, the UV-vis absorption intensity of the side-by-side aggregates was changed in the presence of the target analyte. This assay allowed the selective determination of GM in the range of 0.1-20 ng/mL, and the limit of detection (LOD) of GM was 0.05 ng/mL. Furthermore, compared with the traditional plate-based immunoassay, the developed method was easy to perform without washing cycles and the results could be read as soon as the nanoprobe-analyte incubation was complete. Therefore, the developed method could be a promising tool for the detection of antibiotic residues.
authors.affiliation: School of Food Science and Technology, State Key Lab of Food Science and Technology, Jiangnan University, Wuxi, JiangSu 214122, PR China.
authors.forename: Yingyue
authors.initials: Y
authors.lastname: Zhu
authors.forename: Changlong
authors.initials: C
authors.lastname: Qu
authors.forename: Hua
authors.initials: H
authors.lastname: Kuang
authors.forename: Liguang
authors.initials: L
authors.lastname: Xu
authors.forename: Liqiang
authors.initials: L
authors.lastname: Liu
authors.forename: Yufei
authors.initials: Y
authors.lastname: Hua
authors.forename: Libing
authors.initials: L
authors.lastname: Wang
authors.forename: Chuanlai
authors.initials: C
authors.lastname: Xu
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D015374:Biosensing Techniques / Q000379:methods* / Q000706:statistics & numerical data; D004350:Drug Residues / Q000032:analysis; D005506:Food Contamination / Q000032:analysis; D005839:Gentamicins / Q000032:analysis; D006046:Gold; D053768:Metal Nanoparticles / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D036103:Nanotechnology; D043942:Nanotubes / Q000648:ultrastructure; D010047:Ovalbumin; D013053:Spectrophotometry
chemical_list: D000900:Anti-Bacterial Agents; D005839:Gentamicins; D006046:Gold; D010047:Ovalbumin