title: The realistic prediction of oxygen transport in a tissue-engineered scaffold by introducing time-varying effective diffusion coefficients.
abstract: An adequate oxygen supply is one of the most important factors needed in order to regenerate or engineer thick tissues or complex organs. To devise a method for maximizing the amount of oxygen available to cells, it is necessary to understand and to realistically predict oxygen transport within an engineered tissue. In this study, we focused on the fact that oxygen transport through a tissue-engineered scaffold may vary with time as cells proliferate. To confirm this viewpoint, effective oxygen diffusion coefficients (D(e)(,)(s)) of scaffolds were deduced from experimental measurements and simulations of oxygen-concentration profiles were performed using these D(e)(,)(s) values in a two-dimensional (2-D) perfusion model. The results of this study indicate that higher porosity, hydraulic permeability and interconnectivity of scaffolds with no cells are responsible for the prominent diffusion capability quantified using D(e)(,)(s). On the other hand, the D(e)(,)(s) of scaffolds with cells has a negative linear relationship with cell density. Cell proliferation with time leads to a significant decrease in oxygen concentration in the 2-D perfusion model. This result demonstrates the gradual restriction of oxygen transport in a porous scaffold during cell culture. Therefore, the realistic prediction of oxygen transport using a time-varying D(e)(,)(s) will provide an appropriate basis for designing optimal transport networks within a thick scaffold.
authors.affiliation: Department of Mechanical Engineering, Pohang University of Science and Technology, Gyungbuk, Republic of Korea.
authors.forename: Tae-Yun
authors.initials: TY
authors.lastname: Kang
authors.forename: Hyun-Wook
authors.initials: HW
authors.lastname: Kang
authors.forename: Chang Mo
authors.initials: CM
authors.lastname: Hwang
authors.forename: Sang Jin
authors.initials: SJ
authors.lastname: Lee
authors.forename: Jaesung
authors.initials: J
authors.lastname: Park
authors.forename: James J
authors.initials: JJ
authors.lastname: Yoo
authors.forename: Dong-Woo
authors.initials: DW
authors.lastname: Cho
mesh_terms: D000818:Animals; D001692:Biological Transport; D049109:Cell Proliferation; D051379:Mice; D008855:Microscopy, Electron, Scanning; D041681:NIH 3T3 Cells; D010100:Oxygen / Q000378:metabolism*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D010100:Oxygen