title: [Effects of Feiyanning on the expression of epithelial-mesenchymal factors in highly metastatic lung cancer cells 95-D].
abstract: OBJECTIVE
  Our previous in vivo experiment (paper has been published) has confirmed Feiyanning's regulation effect on epithelial cell markers Alpha-catenin, beta-catenin, E-Cadherin and mesenchymal cell markers N-cadherin, Fibronectin, vimentin. Based on previous study, the objective of this study is to further investigate the expression of epithelial-mesenchymal cell marker gene and protein intervened by Feiyanning in highly metastatic lung cancer cells 95-D in vitro.
  
  
  METHODS
  Human highly metastatic lung cancer 95-D cells were treated with different concentrations of Feiyanning in vitro, and then Real-time PCR and Western blot methods were used to detect mRNA and protein expression of epithelial-mesenchymal cell marker factor Alpha-catenin, beta-catenin, E-Cadherin, N-cadherin, Fibronectin and vimentin.
  
  
  RESULTS
  Real-time PCR results showed that compared with the control group, Feiyanning at 20% concentration remarkably up-regulated the expression of Alpha-catenin (P<0.05), Feiyanning at 20% and 25% concentrations remarkably up-regulated the expression of E-Cadherin (P<0.05, P<0.01), Feiyanning had no significantly effect on beta-catenin at any concentration, 5% and 10% Feiyanning at 5% and 10% concentrations significantly dow-regulated the expression of mesenchymal cell marker vimentin factor (P<0.01), Feiyanning had no regulatory role on N-cadherin and Fibronectin at any concentration. Western blot results showed that Feiyanning at 5%, 10%, 15%, 20% and 25% concentrations significantly increased E-Cadherin protein expression (P<0.01), but had no regulation effect on Alpha-catenin and beta-catenin protein expression; Feiyanning at 5% and 10% concentrations had a down-regulation effect on N-cadherin and Fibronectin protein expression (P<0.01), and had no regulation effect on vimentin protein expression.
  
  
  CONCLUSIONS
  Feiyanning play a role in inhibiting the adhesion of tumor heterogeneity and the athletic ability by regulating epithelial-mesenchymal cell marker factor expression, and thus inhibit the invasion and metastasis of lung cancer.
authors.affiliation: Department of Oncology Section of Longhua Hospital, Cancer Institute of Chinese Medicine, and Science and Technology Experiment Center, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China. zhxzhtiger2001@yahoo.com.cn
authors.forename: Xiaozhen
authors.initials: X
authors.lastname: Zhao
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Wu
authors.forename: Zhenye
authors.initials: Z
authors.lastname: Xu
authors.forename: Zhongqi
authors.initials: Z
authors.lastname: Wang
authors.forename: Ji
authors.initials: J
authors.lastname: Wu
authors.forename: Wan
authors.initials: W
authors.lastname: Su
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D009362:Neoplasm Metastasis
chemical_list: D014408:Biomarkers, Tumor; D004365:Drugs, Chinese Herbal