title: Pulmonary exposure to carbon black by inhalation or instillation in pregnant mice: effects on liver DNA strand breaks in dams and offspring.
abstract: Effects of maternal pulmonary exposure to carbon black (Printex 90) on gestation, lactation and DNA strand breaks were evaluated. Time-mated C57BL/6BomTac mice were exposed by inhalation to 42 mg/m(3) Printex 90 for 1 h/day on gestation days (GD) 8-18, or by four intratracheal instillations on GD 7, 10, 15 and 18, with total doses of 11, 54 and 268 μg/animal. Dams were monitored until weaning and some offspring until adolescence. Inflammation was assessed in maternal bronchoalveolar lavage (BAL) 3-5 days after exposure, and at weaning. Levels of DNA strand breaks were assessed in maternal BAL cells and liver, and in offspring liver. Persistent lung inflammation was observed in exposed mothers. Inhalation exposure induced more DNA strand breaks in the liver of mothers and their offspring, whereas intratracheal instillation did not. Neither inhalation nor instillation affected gestation and lactation. Maternal inhalation exposure to Printex 90-induced liver DNA damage in the mothers and the in utero exposed offspring.
authors.affiliation: National Research Centre for the Working Environment, Copenhagen, Denmark.
authors.forename: Petra
authors.initials: P
authors.lastname: Jackson
authors.forename: Karin Sørig
authors.initials: KS
authors.lastname: Hougaard
authors.forename: Anne Mette Z
authors.initials: AM
authors.lastname: Boisen
authors.forename: Nicklas Raun
authors.initials: NR
authors.lastname: Jacobsen
authors.forename: Keld Alstrup
authors.initials: KA
authors.lastname: Jensen
authors.forename: Peter
authors.initials: P
authors.lastname: Møller
authors.forename: Gunnar
authors.initials: G
authors.lastname: Brunborg
authors.forename: Kristine Bjerve
authors.initials: KB
authors.lastname: Gutzkow
authors.forename: Ole
authors.initials: O
authors.lastname: Andersen
authors.forename: Steffen
authors.initials: S
authors.lastname: Loft
authors.forename: Ulla
authors.initials: U
authors.lastname: Vogel
authors.forename: Håkan
authors.initials: H
authors.lastname: Wallin
mesh_terms: D000280:Administration, Inhalation; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D056486:Chemical and Drug Induced Liver Injury / Q000209:etiology* / Q000235:genetics; D004249:DNA Damage; D005260:Female; D019570:Inhalation Exposure; D007774:Lactation; D008099:Liver / Q000737:chemistry / Q000187:drug effects; D008297:Male; D018811:Maternal Exposure / Q000009:adverse effects*; D051379:Mice; D008810:Mice, Inbred C57BL; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity*; D009929:Organ Size; D011014:Pneumonia / Q000139:chemically induced; D011247:Pregnancy; D053260:Soot / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity*
chemical_list: D053260:Soot