title: Oligosaccharide dehydrogenase-modified graphite electrodes for the amperometric determination of sugars in a flow injection system.
abstract: Enzyme electrodes for the determination of sugars based on solid graphite electrodes modified with oligosaccharide dehydrogenase "wired" with an osmium-based one-electron (no proton) acceptor redox hydrogel were studied as sensors in a flow injection system. The enzyme and a poly(1-vinylimidazole) (PVI) where every tenth mer is complexed with osmium (4,4'-dimethylbpy)(2)Cl, (denoted PVI(10)dmeOs) were cross-linked with poly(ethylene glycol) (diglycidyl) ether. The electrodes were active for l-arabinose, d-xylose, d-galactose, d-fructose, d-glucose, d-mannose, cellobiose, lactose, maltose, and maltooligosaccharides up to a degree of polymerization of 7. The highest relative response found was for glucose (100%) followed by maltose (40.6%) and lactose (40.6%). Fructose and isomaltotriose gave the lowest responses (<1%). Calibration curves for glucose were strictly linear in the range between 30 and 500 μM with sensitivity and apparent Michaelis-Menten constant (K(m)(app)) of 23.0 ± 1.4 μA mM(-)(1)cm(-)(2) and 4.26 ± 0.95 mM, respectively.
authors.affiliation: Department of Chemistry, Addis Ababa University, P.O. Box 1176, Addis Ababa, Ethiopia, Department of Analytical Chemistry, University of Lund, P.O. Box 124, SE-221 00, Lund, Sweden, Enzyme Chemistry Laboratory, Institute of Biochemistry, Mokslininku 12, LT-2600 Vilnius, Lithuania, and Department of Chemical Engineering, The University of Texas at Austin, Austin, Texas 78712-1062.
authors.forename: M
authors.initials: M
authors.lastname: Tessema
authors.forename: E
authors.initials: E
authors.lastname: Csöregi
authors.forename: T
authors.initials: T
authors.lastname: Ruzgas
authors.forename: G
authors.initials: G
authors.lastname: Kenausis
authors.forename: T
authors.initials: T
authors.lastname: Solomon
authors.forename: L
authors.initials: L
authors.lastname: Gorton