title: Screening of species-specific lactic acid bacteria for veal calves multi-strain probiotic adjuncts.
abstract: The selection of promising specific species of lactic acid bacteria with potential probiotic characteristics is of particular interest in producing multi species-specific probiotic adjuncts in veal calves rearing. The aim of the present work was to select and evaluate in vitro the functional activity of lactic acid bacteria, Bifidobacterium longum and Bacillus coagulans strains isolated from veal calves in order to assess their potential use as multi species-specific probiotics for veal calves. For this purpose, bacterial strains isolated from faeces collected from 40 healthy 50-day-calves, were identified by RiboPrinter and 16s rRNA gene sequence. The most frequent strains belonged to the species B. longum, Streptococcus bovis, Lactobacillus animalis and Streptococcus macedonicus. Among these, 7 strains were chosen for testing their probiotic characteristics in vitro. Three strains, namely L. animalis SB310, Lactobacillus paracasei subsp. paracasei SB137 and B. coagulans SB117 showed varying individual but promising capabilities to survive in the gastrointestinal tract, to adhere, to produce antimicrobial compounds. These three selected species-specific bacteria demonstrated in vitro, both singularly and mixed, the functional properties needed for their use as potential probiotics in veal calves.
authors.affiliation: Università degli Studi di Milano, Department of Veterinary Science and Technology for Food Safety, Milan, Italy.
authors.forename: Barbara
authors.initials: B
authors.lastname: Ripamonti
authors.forename: Alessandro
authors.initials: A
authors.lastname: Agazzi
authors.forename: Carla
authors.initials: C
authors.lastname: Bersani
authors.forename: Paola
authors.initials: P
authors.lastname: De Dea
authors.forename: Chiara
authors.initials: C
authors.lastname: Pecorini
authors.forename: Silvia
authors.initials: S
authors.lastname: Pirani
authors.forename: Raffaella
authors.initials: R
authors.lastname: Rebucci
authors.forename: Giovanni
authors.initials: G
authors.lastname: Savoini
authors.forename: Simone
authors.initials: S
authors.lastname: Stella
authors.forename: Alberta
authors.initials: A
authors.lastname: Stenico
authors.forename: Erica
authors.initials: E
authors.lastname: Tirloni
authors.forename: Cinzia
authors.initials: C
authors.lastname: Domeneghini
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000898:Antibiosis; D001407:Bacillus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D001422:Bacterial Adhesion; D015373:Bacterial Typing Techniques; D001644:Bifidobacterium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D002417:Cattle / Q000382:microbiology*; D002460:Cell Line; D016000:Cluster Analysis; D005232:Fatty Acids, Volatile / Q000096:biosynthesis; D005243:Feces / Q000382:microbiology; D006801:Humans; D007422:Intestines / Q000382:microbiology; D019344:Lactic Acid / Q000096:biosynthesis*; D007778:Lactobacillus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability; D019936:Probiotics / Q000737:chemistry / Q000302:isolation & purification*; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013045:Species Specificity
chemical_list: D005232:Fatty Acids, Volatile; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D019344:Lactic Acid