title: High spatial resolution imaging mass spectrometry and classical histology on a single tissue section.
abstract: The work presented in this report describes and demonstrates a protocol for protein imaging analysis of biological tissue using MALDI IMS where histological staining and MS analysis are performed on the same tissue section. Spatial image resolution is shown at 35 µm for sagittal sections of the cerebellum from rat brain.
authors.affiliation: Department of Biochemistry, Mass Spectrometry Research Center, Vanderbilt University, Nashville, TN, USA.
authors.forename: Fabian
authors.initials: F
authors.lastname: Deutskens
authors.forename: Junhai
authors.initials: J
authors.lastname: Yang
authors.forename: Richard M
authors.initials: RM
authors.lastname: Caprioli
mesh_terms: D000818:Animals; D002531:Cerebellum / Q000737:chemistry; D005260:Female; D006651:Histocytochemistry / Q000379:methods*; D016591:Histocytological Preparation Techniques; D008297:Male; D057054:Molecular Imaging / Q000379:methods*; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D009419:Nerve Tissue Proteins