title: Expression of hexose-6-phosphate dehydrogenase in rat tissues.
abstract: Hexose-6-phosphate dehydrogenase (H6PD) is the main NADPH generating enzyme in the lumen of the endoplasmic reticulum. H6PD is regarded as an ancillary enzyme in prereceptorial glucocorticoid activation and probably acts as a nutrient sensor and as a prosurvival factor. H6PD expression was determined in a variety of rat and human tissues by detecting mRNA and protein levels, and by measuring its dehydrogenase and lactonase activities. It was found that H6PD was present in all investigated tissues; both expression and activity remained within an order of magnitude. Correlation was found between the dehydrogenase activity and protein or mRNA levels. The results confirmed the supposed housekeeping feature of the enzyme.
authors.affiliation: Dipartimento di Fisiopatologia, Medicina Sperimentale e Sanità Pubblica, Università di Siena, Viale Aldo Moro, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Marcolongo
authors.forename: Silvia
authors.initials: S
authors.lastname: Senesi
authors.forename: Roberta
authors.initials: R
authors.lastname: Giunti
authors.forename: Miklós
authors.initials: M
authors.lastname: Csala
authors.forename: Rosella
authors.initials: R
authors.lastname: Fulceri
authors.forename: Gábor
authors.initials: G
authors.lastname: Bánhegyi
authors.forename: Angelo
authors.initials: A
authors.lastname: Benedetti
mesh_terms: D000818:Animals; D002237:Carbohydrate Dehydrogenases / Q000235:genetics* / Q000378:metabolism*; D057075:Enzyme Assays; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D008861:Microsomes / Q000737:chemistry / Q000378:metabolism; D016133:Polymerase Chain Reaction; D051381:Rats / Q000235:genetics* / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D002237:Carbohydrate Dehydrogenases; C020552:galactose-6-phosphate dehydrogenase