title: Variants and haplotypes in angiotensinogen gene are associated with plasmatic angiotensinogen level in Mexican population.
abstract: BACKGROUND
  The plasmatic angiotensinogen (AGT) level has been associated with essential hypertension. Linkage analysis has found a relationship between the AGT gene locus and hypertension in the Mexican-American population, but studies have failed to identify genetic variants associated with hypertension or plasma AGT levels. This study analyzes the relationship between polymorphisms in the AGT gene and plasmatic AGT levels in Mexican population.
  
  
  METHODS
  Nine polymorphisms in AGT gene were genotyped, and plasma AGT level was determined by enzyme-linked immunosorbent assay.
  
  
  RESULTS
  Differences in AGT plasma levels were associated with 2 polymorphisms: T-20G, TT = 25.3 ± 8.3 versus TG + GG = 21.6 ± 8.8 μg/mL; P = 0.008 and C3389T (T174M), CC = 25.8 ± 9.9 versus TC + TT = 20.5 ± 5.4 μg/mL; P = 0.0002. Haplotype 2 was associated with low plasma AGT (-5.1 μg/mL [95% confidence interval: -8.6 to -1.6], P = 0.004) and Haplotype 8 was associated with high plasma AGT (6.5 μg/mL [95% confidence interval: 2.5 to 10.6], P = 0.001). This association remained after adjustment for covariates. A Likelihood Ratio Test for haplotype-phenotype association adjusted for covariates resulted in χ = 38.9, P = 0.0005. The total effect of the haplotypes on plasma AGT level variance was 19.5%. No association was identified between haplotypes and quantitative traits of blood pressure.
  
  
  CONCLUSIONS
  Two polymorphisms (T-20G and C3389T) and 2 haplotypes (H2 and H8) showed an association with plasma AGT levels in Mexican population.
authors.affiliation: Division of Cardiovascular Genomics, National Institute of Genomic Medicine (INMEGEN), Mexico City, Mexico. ebalam@inmegen.gob.mx
authors.forename: Eros
authors.initials: E
authors.lastname: Balam-Ortiz
authors.forename: Adolfo
authors.initials: A
authors.lastname: Esquivel-Villarreal
authors.forename: Luis
authors.initials: L
authors.lastname: Alfaro-Ruiz
authors.forename: Karol
authors.initials: K
authors.lastname: Carrillo
authors.forename: Adela
authors.initials: A
authors.lastname: Elizalde
authors.forename: Trinidad
authors.initials: T
authors.lastname: Gil
authors.forename: Maki
authors.initials: M
authors.lastname: Urushihara
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kobori
authors.forename: Gerardo
authors.initials: G
authors.lastname: Jimenez-Sanchez
mesh_terms: D000368:Aged; D000808:Angiotensinogen / Q000097:blood / Q000235:genetics*; D001794:Blood Pressure / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005796:Genes / Q000235:genetics; D056726:Genetic Association Studies; D005838:Genotype; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D006973:Hypertension / Q000235:genetics; D008297:Male; D008800:Mexico; D009765:Obesity / Q000235:genetics; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
chemical_list: D000808:Angiotensinogen