title: Viral agents causing lower respiratory tract infections in hospitalized children: evaluation of the Speed-Oligo® RSV assay for the detection of respiratory syncytial virus.
abstract: Respiratory syncytial virus (RSV) is the viral agent which is more frequently involved in lower respiratory tract infections (LRTIs) in infants under 1 year of age in developed countries. A new oligochromatographic assay, Speed-Oligo® RSV, was designed and optimized for the specific detection and identification of RSV subtypes A and B. The test was evaluated in 289 clinical samples from 169 hospitalized children using an immunochromatography (IC) test, virus isolation by culture, and an in-house real-time polymerase chain reaction (RT-PCR). Other viruses causing LRTIs were investigated by cell culture or PCR-based tests. Sixty-two patients were infected by RSV (36.7%). In addition, adenovirus, influenza B, parainfluenza 2, and human metapneumovirus were detected in rates ranging from 5 to 8%. A proportion of 10.1% of the patients had mixed infections. The sensitivity, specificity, and positive and negative predictive values were, respectively, 94.9, 99.4, 98.9, and 97.4% for Speed-Oligo® RSV, 92.9, 96.3, 92.9, and 96.3% for RT-PCR/RSV, and 58.4, 98.1, 93.3, and 82.6% for IC. Our rates of viral detection and co-infection were similar to those of previously reported series. Finally, we find that Speed-Oligo® RSV is a rapid and easy-to-perform technique for the detection of RSV and the identification of subtypes A and B.
authors.affiliation: Department of Microbiology, CH Torrecárdenas, 04009, Almería, Spain. wsyebra@telefonica.net
authors.forename: W
authors.initials: W
authors.lastname: Sánchez-Yebra
authors.forename: J A
authors.initials: JA
authors.lastname: Ávila-Carrillo
authors.forename: F
authors.initials: F
authors.lastname: Giménez-Sánchez
authors.forename: A
authors.initials: A
authors.lastname: Reyes-Bertos
authors.forename: M
authors.initials: M
authors.lastname: Sánchez-Forte
authors.forename: M
authors.initials: M
authors.lastname: Morales-Torres
authors.forename: A
authors.initials: A
authors.lastname: Rojas
authors.forename: J
authors.initials: J
authors.lastname: Mendoza
mesh_terms: D000293:Adolescent; D001990:Bronchiolitis, Viral / Q000175:diagnosis* / Q000821:virology; D002648:Child; D002673:Child, Hospitalized; D002675:Child, Preschool; D003948:Diagnostic Techniques, Respiratory System; D006760:Hospitalization; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D016133:Polymerase Chain Reaction / Q000379:methods; D011237:Predictive Value of Tests; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics; D018357:Respiratory Syncytial Virus Infections / Q000175:diagnosis* / Q000821:virology; D018113:Respiratory Syncytial Virus, Human / Q000235:genetics* / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity
chemical_list: D012367:RNA, Viral