title: High-throughput multispot single-molecule spectroscopy.
abstract: Solution-based single-molecule spectroscopy and fluorescence correlation spectroscopy (FCS) are powerful techniques to access a variety of molecular properties such as size, brightness, conformation, and binding constants. However, this is limited to low concentrations, which results in long acquisition times in order to achieve good statistical accuracy. Data can be acquired more quickly by using parallelization. We present a new approach using a multispot excitation and detection geometry made possible by the combination of three powerful new technologies: (i) a liquid crystal spatial light modulator to produce multiple diffraction-limited excitation spots; (ii) a multipixel detector array matching the excitation pattern and (iii) a low-cost reconfigurable multichannel counting board. We demonstrate the capabilities of this technique by reporting FCS measurements of various calibrated samples as well as single-molecule burst measurements.
authors.affiliation: Department of Chemistry & Biochemistry, UCLA, Los Angeles, CA.
authors.forename: Ryan A
authors.initials: RA
authors.lastname: Colyer
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Scalia
authors.forename: Taiho
authors.initials: T
authors.lastname: Kim
authors.forename: Ivan
authors.initials: I
authors.lastname: Rech
authors.forename: Daniele
authors.initials: D
authors.lastname: Resnati
authors.forename: Stefano
authors.initials: S
authors.lastname: Marangoni
authors.forename: Massimo
authors.initials: M
authors.lastname: Ghioni
authors.forename: Sergio
authors.initials: S
authors.lastname: Cova
authors.forename: Shimon
authors.initials: S
authors.lastname: Weiss
authors.forename: Xavier
authors.initials: X
authors.lastname: Michalet