title: Cell adhesion and proliferation studies on semi-interpenetrating polymeric networks (semi-IPNs) of polyacrylamide and gelatin.
abstract: In this study, the effect of feed composition, degree of hydrophilicity, and internal morphology has been investigated for cell proliferation potential of the polyacrylamide/gelatin (PAm/G) semi-interpenetrating polymeric network (semi-IPNs). Polycaprolactone diacrylate was used to cross-link polyacrylamide chains. Scanning electron microscopy (SEM) micrographs demonstrate uniformly distributed porous structure with internal diameter in the range of 75-175 μm, dependent on matrix compositions. Water-air contact angle was found in the range of 49° ± 0.22 to 89° ± 0.14 (p < 0.02) suggesting varying degree of hydrophilicity of the hydrogel surface. In addition, protein adsorption study showed 45 ± 0.14 μg to 64 ± 0.12 μg (p < 0.01) of protein adsorbed per cm² of hydrogel. Quantitative estimation of cell adhesion and proliferation was carried out by DNA quantification using fluorimetric assay method (p < 0.02). Microscopic images of proliferative cells on semi-IPNs by fluorescent and inverted phase contrast supported the findings of DNA quantification. Contact angle in the range of 63-69° in association with 52-59 μg/cm² protein absorption and 115-150 μm pore size was found optimum for fibroblast proliferation on PAm/G semi-IPN scaffolds. The newly developed semi-interpenetrating network may serve as a potential scaffold for soft tissue-engineering applications.
authors.affiliation: Centre for Biomedical Engineering, Indian Institute of Technology, New Delhi 110016, India.
authors.forename: Maneesh
authors.initials: M
authors.lastname: Jaiswal
authors.forename: Veena
authors.initials: V
authors.lastname: Koul
authors.forename: Amit K
authors.initials: AK
authors.lastname: Dinda
authors.forename: Sujata
authors.initials: S
authors.lastname: Mohanty
authors.forename: Krishan Gopal
authors.initials: KG
authors.lastname: Jain
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000327:Adsorption; D000818:Animals; D002448:Cell Adhesion; D049109:Cell Proliferation; D003432:Cross-Linking Reagents; D005347:Fibroblasts / Q000166:cytology; D005780:Gelatin / Q000737:chemistry*; D006801:Humans; D010316:Particle Size; D016062:Porosity; D011506:Proteins; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D003432:Cross-Linking Reagents; D005780:Gelatin; D011506:Proteins; D000099166:Polyacrylamides