title: Regulation of expression of trehalose-6-phosphate synthase during cold shock in Arthrobacter strain A3.
abstract: Trehalose is a chemical chaperone known to protect a variety of organisms against cold stress. Members of the genus Arthrobacter, which belongs to the Actinomycetales group, exhibit strong resistance to stress conditions, but exactly how trehalose synthesis is regulated in conditions of cold stress is still unknown. Here, we report that Arthrobacter strain A3, which was isolated from the alpine permafrost, has only two trehalose synthesis pathways (OtsA/B and TreS), while other Arthrobacter spp. have three. Mutants and immunoblot analyses indicate that trehalose is mainly synthesized via OtsA at low temperatures in Arthrobacter strain A3. Therefore, we have focused on the regulation of OtsA expression during cold shock. The results indicated that both low temperature and accumulation of trehalose can inhibit OtsA expression. The elongation factor Tu, which binds to OtsA, stabilizes the expression of OtsA in the cold.
authors.affiliation: State Key Laboratory of Arid and Grassland Agroecology of Ministry of Education, Lanzhou University, People's Republic of China.
authors.forename: Xi-Ming
authors.initials: XM
authors.lastname: Chen
authors.forename: Ying
authors.initials: Y
authors.lastname: Jiang
authors.forename: Yuan-Ting
authors.initials: YT
authors.lastname: Li
authors.forename: Hai-Hong
authors.initials: HH
authors.lastname: Zhang
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.forename: Xing
authors.initials: X
authors.lastname: Chen
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhao
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.forename: Jing
authors.initials: J
authors.lastname: Si
authors.forename: Zhi-Wei
authors.initials: ZW
authors.lastname: Lin
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
authors.forename: Paul
authors.initials: P
authors.lastname: Dyson
authors.forename: Li-Zhe
authors.initials: LZ
authors.lastname: An
mesh_terms: D001173:Arthrobacter / Q000201:enzymology*; D001426:Bacterial Proteins / Q000096:biosynthesis*; D058639:Cold-Shock Response / Q000502:physiology*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D005964:Glucosyltransferases / Q000096:biosynthesis*; D014199:Trehalose / Q000096:biosynthesis
chemical_list: D001426:Bacterial Proteins; D014199:Trehalose; D005964:Glucosyltransferases; C023180:trehalose-6-phosphate synthase