title: Aphids preserved in propylene glycol can be used for reverse transcription-polymerase chain reaction detection of Potato virus Y.
abstract: The effectiveness of propylene glycol on the retention of RNA target of Potato virus Y (PVY), an aphid stylet-borne virus, in Myzus persicae was investigated in comparison to ethanol and liquid nitrogen/-80°C. Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the PVY targets from the propylene glycol/ethanol/liquid nitrogen preserved single aphids after a 5min acquisition period from infected potato plants. In the liquid nitrogen/-80°C and 70% ethanol treatments, 55.6% and 38.8% aphids tested PVY-positive, respectively. In the 0-75% propylene glycol treatments, 12.2-44.7% aphids tested PVY-positive. The lowest detection rate was in the 0% (positive rate, 15.2%) and the 10% propylene glycol (positive rate, 12.2%). As the propylene glycol concentration increased to 25%, 29.8% aphids tested positive. A high PVY-positive rate was also found in 35-75% propylene glycol treatments at 44.7% (35% propylene glycol), 36.7% (50% propylene glycol) and 34.8% (75% propylene glycol), which is comparable to the rate shown in 70% ethanol. No significant difference in the positive detection rate was observed in aphids preserved in 50% propylene glycol at room temperature for 2, 4 and 10 days. These results demonstrate that propylene glycol at 25-75% can retain PVY targets effectively in aphids for an extended time period, and thus can be used in aphid traps to preserve viruliferous aphids for later RT-PCR detection of PVY.
authors.affiliation: Potato Research Centre, Agriculture and Agri-Food Canada, P.O. Box 20280, 850 Lincoln Road, Fredericton, New Brunswick, Canada E3B 4Z7. xianzhou.nie@agr.gc.ca
authors.forename: Xianzhou
authors.initials: X
authors.lastname: Nie
authors.forename: Yvan
authors.initials: Y
authors.lastname: Pelletier
authors.forename: Nicola
authors.initials: N
authors.lastname: Mason
authors.forename: Andrea
authors.initials: A
authors.lastname: Dilworth
authors.forename: Marie-Andrée
authors.initials: MA
authors.lastname: Giguère
mesh_terms: D000818:Animals; D001042:Aphids / Q000821:virology*; D015925:Cryopreservation / Q000379:methods*; D017800:Potyvirus / Q000302:isolation & purification*; D019946:Propylene Glycol; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*
chemical_list: D019946:Propylene Glycol