title: Using 2-DE for the differentiation of local chicken breeds.
abstract: The aim of this study was to apply a proteomic approach for the characterisation of local chicken breeds. The experiment involved a total of 29 males of three local Italian chicken breeds: Pépoi, Padovana and Ermellinata di Rovigo. Sarcoplasmic protein fractions of breast muscle were analysed by 2-DE. Image analysis followed by statistical analysis enabled to differentiate groups of individuals based on the similarities of protein expression. Individuals were distinguished into clusters and groups, corresponding to the breed of origin. Distances among individuals, calculated using data on spot volumes, were used to draw a neighbour-joining tree, showing clear individual and breed grouping. The most relevant spots regarding breed differentiation were detected; 11 were identified by MS revealing preliminary evidences on the mechanisms of the breed differentiation process. The results evidenced the ability of proteomic analyses for the characterisation of chicken breeds.
authors.affiliation: Dipartimento di Scienze Animali, Università di Padova, Padova, Italy. enrico.zanetti@unipd.it
authors.forename: Enrico
authors.initials: E
authors.lastname: Zanetti
authors.forename: Caroline
authors.initials: C
authors.lastname: Molette
authors.forename: Christophe
authors.initials: C
authors.lastname: Chambon
authors.forename: Jeremy
authors.initials: J
authors.lastname: Pinguet
authors.forename: Hervé
authors.initials: H
authors.lastname: Rémignon
authors.forename: Martino
authors.initials: M
authors.lastname: Cassandro
mesh_terms: D000818:Animals; D001947:Breeding; D002645:Chickens / Q000145:classification* / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D014644:Genetic Variation; D006801:Humans; D008297:Male; D010802:Phylogeny; D011506:Proteins / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D011506:Proteins