title: Mesothelin and GPR30 staining among a spectrum of pancreatic epithelial neoplasms.
abstract: BACKGROUND
  Our study attempts to characterize mesothelin and GPR30 / estrogen receptor (ER) staining in pancreatic pathology.
  
  
  METHODS
  Immunohistochemical staining for mesothelin, GPR30, and ER was performed on a variety of pancreatic lesions.
  
  
  RESULTS
  24 of 42 (57%) adenocarcinomas stained for mesothelin, while 0 of 16 non-carcinomas (0%) stained (p = 0.0000784). 35 of 39 (90%) adenocarcinomas stained for GPR30, while only 4 of 15 (27%) non-carcinomas stained (p = 0.0000036). Apart from stromal staining in one case of mucinous cystic neoplasm, no cases stained for ER. 27 of 37 (73%) adenocarcinoma fine needle aspirates were positive for mesothelin.
  
  
  CONCLUSIONS
  GPR30 is more sensitive, but less specific than mesothelin for pancreatic adenocarcinoma. Mesothelin is detected in most adenocarcinoma fine needle aspirates. ER is rarely detected in pancreatic lesions.
authors.affiliation: University of New Mexico, Albuquerque, NM, USA. joseph.glass2@va.gov
authors.forename: Joseph P
authors.initials: JP
authors.lastname: Glass
authors.forename: Gulshan
authors.initials: G
authors.lastname: Parasher
authors.forename: Hugo
authors.initials: H
authors.lastname: Arias-Pulido
authors.forename: Rachel
authors.initials: R
authors.lastname: Donohue
authors.forename: Eric R
authors.initials: ER
authors.lastname: Prossnitz
authors.forename: Lisa A
authors.initials: LA
authors.lastname: Cerilli
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D001707:Biopsy, Needle; D058851:GPI-Linked Proteins / Q000032:analysis* / Q000096:biosynthesis; D006801:Humans; D007150:Immunohistochemistry; D000090204:Mesothelin; D060787:Neoplasm Grading; D009375:Neoplasms, Glandular and Epithelial / Q000378:metabolism* / Q000473:pathology; D010190:Pancreatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D011960:Receptors, Estrogen / Q000032:analysis* / Q000096:biosynthesis; D043562:Receptors, G-Protein-Coupled / Q000032:analysis* / Q000096:biosynthesis; D012680:Sensitivity and Specificity
chemical_list: D014408:Biomarkers, Tumor; C109584:GPER1 protein, human; D058851:GPI-Linked Proteins; D011960:Receptors, Estrogen; D043562:Receptors, G-Protein-Coupled; D000090204:Mesothelin