title: Isolation and phylogenetic classification of culturable psychrophilic prokaryotes from the Collins glacier in the Antarctica.
abstract: Culturable psychrophilic prokaryotes were obtained of samples of glacier sediment, seaside mud, glacier melted ice, and Deschampsia antarctica rhizosphere from Collins glacier, Antarctica. The taxonomic classification was done by a culture-dependent molecular approach involving the Amplified Ribosomal DNA Restriction Analysis. Two hundred sixty colonies were successfully isolated and sub-cultivated under laboratory conditions. The analysis showed a bacterial profile dominated by Beta-proteobacteria (35.2%) followed by Gamma-proteobacteria (18.5%), Alpha-proteobacteria (16.6%), Gram-positive with high GC content (13%), Cytophaga-Flavobacterium-Bacteroides (13%) and Gram-positive with low GC content (3.7%). Eleven of the isolates have been reported previously and the others microorganisms remain uncharacterized. The isolated microorganisms here could be a potential source for biotechnological products, such as cold-active enzymes and secondary metabolites.
authors.affiliation: División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica, A.C., Camino a la Presa San José 2055, Col. Lomas 4a. Sección, C.P. 78216, San Luis Potosí, SLP, Mexico.
authors.forename: S A
authors.initials: SA
authors.lastname: García-Echauri
authors.forename: M
authors.initials: M
authors.lastname: Gidekel
authors.forename: A
authors.initials: A
authors.lastname: Gutiérrez-Moraga
authors.forename: L
authors.initials: L
authors.lastname: Santos
authors.forename: A
authors.initials: A
authors.lastname: De León-Rodríguez
mesh_terms: D000864:Antarctic Regions; D001439:Bacteroides / Q000235:genetics* / Q000302:isolation & purification*; D001688:Biological Products / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004275:DNA, Ribosomal / Q000032:analysis / Q000235:genetics; D019015:Geologic Sediments / Q000382:microbiology*; D046448:Ice Cover / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D020560:Proteobacteria / Q000235:genetics* / Q000302:isolation & purification*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D001688:Biological Products; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S