title: Telomere length in myelodysplastic syndromes.
abstract: The relationship between telomere length (TL) and predisposition to myelodysplastic syndromes (MDS) remains unclear. We compared peripheral blood leukocyte (PBL) TL among cases of histologically confirmed MDS (n = 65) who were treatment-naive with no prior cancer history to age-matched controls (n = 63). Relative TL was measured in PBLs and saliva by quantitative polymerase chain reaction (PCR) and in CD15+ and CD19+ cells by flow cytometry-fluorescence in situ hybridization (flow-FISH). Human telomerase reverse transcriptase gene (hTERT) mutations were assessed by PCR. After adjustment for age and sex, relative TLs were reduced in PBLs (p = 0.02), CD15+ (p = 0.01), CD19+ (p = 0.25), and saliva (p = 0.13) in MDS cases versus controls, although only the PBL and CD15+ results were statistically significant. Among MDS cases, CD15+ and CD19+ cell TLs were positively correlated (p = 0.03). PBL TL was reduced among those occupationally exposed to paints and pesticides, but was not associated with hTERT genotype. Future studies are needed to further investigate constitutional telomere attrition as a possible predisposing factor for MDS.
authors.affiliation: Division of Cancer Prevention and Control, Moffitt Cancer Center, Tampa, FL 33612, USA. dana.rollison@moffitt.org
authors.forename: Dana E
authors.initials: DE
authors.lastname: Rollison
authors.forename: P K
authors.initials: PK
authors.lastname: Epling-Burnette
authors.forename: Jong Y
authors.initials: JY
authors.lastname: Park
authors.forename: Ji-Hyun
authors.initials: JH
authors.lastname: Lee
authors.forename: Hyun
authors.initials: H
authors.lastname: Park
authors.forename: Kristen
authors.initials: K
authors.lastname: Jonathan
authors.forename: Ashley L
authors.initials: AL
authors.lastname: Cole
authors.forename: Jeffrey S
authors.initials: JS
authors.lastname: Painter
authors.forename: Mayenha
authors.initials: M
authors.lastname: Guerrier
authors.forename: Johana
authors.initials: J
authors.lastname: Meléndez-Santiago
authors.forename: William
authors.initials: W
authors.lastname: Fulp
authors.forename: Rami
authors.initials: R
authors.lastname: Komrokji
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Lancet
authors.forename: Alan F
authors.initials: AF
authors.lastname: List
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018941:Antigens, CD19 / Q000097:blood; D016022:Case-Control Studies; D005260:Female; D005434:Flow Cytometry; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007962:Leukocytes / Q000378:metabolism; D016256:Lewis X Antigen / Q000097:blood; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D009154:Mutation; D009190:Myelodysplastic Syndromes / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D010150:Paint / Q000506:poisoning; D010575:Pesticides / Q000506:poisoning; D016133:Polymerase Chain Reaction; D012463:Saliva / Q000378:metabolism; D019098:Telomerase / Q000235:genetics*; D016615:Telomere / Q000187:drug effects / Q000235:genetics*; D055815:Young Adult
chemical_list: D018941:Antigens, CD19; D016256:Lewis X Antigen; D010575:Pesticides; C509186:TERT protein, human; D019098:Telomerase