title: Human metapneumovirus and human bocavirus associated with respiratory infection in Apulian population.
abstract: We have studied the occurrence of hBoV, hMPV and InfA-B in an Apulian population with respiratory tract infections. During influenza season 2008-2009, 116 oropharingeal swabs were collected from patients affected by Influenza-Like Illness (ILI). The PCR products of hMPV M and HBoV NP-1 genes were sequenced. 78 out of 116 samples were positive for at least one respiratory virus; hBoV was detected in 53, hMPV in 22 and InfA-B in 41 out of 116 swabs. A high rate of hBoV infection in adult (18.9%) and elderly (26.4%) subjects was found. The co-infection rate was higher for hMPV (18/22 cases, 81.8%) compared to hBoV (26/53 cases, 49.1%), and InfA-B (25/41 cases, 61.0%). Co-infections were common in children. hBoV positive samples shared a high level of genetic similarity with the hBoV1 genotype, and hMPV positive samples clustered with A2 subgroup. Our results suggest that hBoV and hMPV play a role in ILI.
authors.affiliation: Laboratory of Hygiene, Department of Biological and Environmental Sciences and Technologies, Faculty of Sciences, University of Salento, Lecce, Italy. marcello.guido@unisalento.it
authors.forename: M
authors.initials: M
authors.lastname: Guido
authors.forename: M
authors.initials: M
authors.lastname: Quattrocchi
authors.forename: A
authors.initials: A
authors.lastname: Campa
authors.forename: A
authors.initials: A
authors.lastname: Zizza
authors.forename: P
authors.initials: P
authors.lastname: Grima
authors.forename: A
authors.initials: A
authors.lastname: Romano
authors.forename: A
authors.initials: A
authors.lastname: De Donno
mesh_terms: D000293:Adolescent; D000328:Adult; D017677:Age Distribution; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D005260:Female; D056661:Human bocavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007223:Infant; D007251:Influenza, Human / Q000453:epidemiology / Q000821:virology; D007558:Italy / Q000453:epidemiology; D008297:Male; D029121:Metapneumovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D008875:Middle Aged; D009975:Orthomyxoviridae / Q000145:classification / Q000302:isolation & purification; D018184:Paramyxoviridae Infections / Q000453:epidemiology / Q000821:virology*; D010322:Parvoviridae Infections / Q000453:epidemiology / Q000821:virology*; D010802:Phylogeny; D011446:Prospective Studies; D012141:Respiratory Tract Infections / Q000453:epidemiology / Q000821:virology*; D055815:Young Adult