title: Desorption electrospray ionization mass spectrometry for lipid characterization and biological tissue imaging.
abstract: Desorption electrospray ionization mass spectrometry (DESI-MS) imaging of biological samples allows untargeted analysis and structural characterization of lipids ionized from the near-surface region of a sample under ambient conditions. DESI is a powerful and sensitive MS ionization method for 2D and 3D imaging of lipids from direct and unmodified complex biological samples. This review describes the strengths and limitations of DESI-MS for lipid characterization and imaging together with the technical workflow and a survey of applications. Included are discussions of lipid mapping and biomarker discovery as well as a perspective on the future of DESI imaging.
authors.affiliation: Department of Chemistry, Purdue University, West Lafayette, IN, USA.
authors.forename: Livia S
authors.initials: LS
authors.lastname: Eberlin
authors.forename: Christina R
authors.initials: CR
authors.lastname: Ferreira
authors.forename: Allison L
authors.initials: AL
authors.lastname: Dill
authors.forename: Demian R
authors.initials: DR
authors.lastname: Ifa
authors.forename: R Graham
authors.initials: RG
authors.lastname: Cooks
mesh_terms: D000818:Animals; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008055:Lipids / Q000032:analysis* / Q000737:chemistry; D009928:Organ Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D008055:Lipids