title: Reversible thrombin detection by aptamer functionalized STING sensors.
abstract: Signal Transduction by Ion NanoGating (STING) is a label-free technology based on functionalized quartz nanopipettes. The nanopipette pore can be decorated with a variety of recognition elements and the molecular interaction is transduced via a simple electrochemical system. A STING sensor can be easily and reproducibly fabricated and tailored at the bench starting from inexpensive quartz capillaries. The analytical application of this new biosensing platform, however, was limited due to the difficult correlation between the measured ionic current and the analyte concentration in solution. Here we show that STING sensors functionalized with aptamers allow the quantitative detection of thrombin. The binding of thrombin generates a signal that can be directly correlated to its concentration in the bulk solution.
authors.affiliation: Department of Biomolecular Engineering, University of California Santa Cruz, 1156 High Street, Santa Cruz, CA 95064, USA.
authors.forename: Paolo
authors.initials: P
authors.lastname: Actis
authors.forename: Adam
authors.initials: A
authors.lastname: Rogers
authors.forename: Jeff
authors.initials: J
authors.lastname: Nivala
authors.forename: Boaz
authors.initials: B
authors.lastname: Vilozny
authors.forename: R Adam
authors.initials: RA
authors.lastname: Seger
authors.forename: Olufisayo
authors.initials: O
authors.lastname: Jejelowo
authors.forename: Nader
authors.initials: N
authors.lastname: Pourmand
mesh_terms: D052157:Aptamers, Nucleotide; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D001774:Blood Chemical Analysis / Q000295:instrumentation / Q000379:methods; D055664:Electrochemical Techniques; D004867:Equipment Design; D006801:Humans; D058608:Nanopores; D036103:Nanotechnology; D015398:Signal Transduction; D013917:Thrombin / Q000032:analysis*
chemical_list: D052157:Aptamers, Nucleotide; D013917:Thrombin