title: Design, development and characterization of multi-functionalized gold nanoparticles for biodetection and targeted boron delivery in BNCT applications.
abstract: The aim of this study is to optimize targeted boron delivery to cancer cells and its tracking down to the cellular level. To this end, we describe the design and synthesis of novel nanovectors that double as targeted boron delivery agents and fluorescent imaging probes. Gold nanoparticles were coated with multilayers of polyelectrolytes functionalized with the fluorescent dye (FITC), boronophenylalanine and folic acid. In vitro confocal fluorescence microscopy demonstrated significant uptake of the nanoparticles in cancer cells that are known to overexpress folate receptors.
authors.affiliation: Department of Tumor Immunology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands.
authors.forename: Subhra
authors.initials: S
authors.lastname: Mandal
authors.forename: Gerald J
authors.initials: GJ
authors.lastname: Bakeine
authors.forename: Silke
authors.initials: S
authors.lastname: Krol
authors.forename: Cinzia
authors.initials: C
authors.lastname: Ferrari
authors.forename: Anna M
authors.initials: AM
authors.lastname: Clerici
authors.forename: Cecilia
authors.initials: C
authors.lastname: Zonta
authors.forename: Laura
authors.initials: L
authors.lastname: Cansolino
authors.forename: Francesca
authors.initials: F
authors.lastname: Ballarini
authors.forename: Silva
authors.initials: S
authors.lastname: Bortolussi
authors.forename: Subrina
authors.initials: S
authors.lastname: Stella
authors.forename: Nicoletta
authors.initials: N
authors.lastname: Protti
authors.forename: Piero
authors.initials: P
authors.lastname: Bruschi
authors.forename: Saverio
authors.initials: S
authors.lastname: Altieri
mesh_terms: D001895:Boron / Q000008:administration & dosage* / Q000032:analysis*; D016754:Boron Neutron Capture Therapy; D045744:Cell Line, Tumor; D005492:Folic Acid / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D006801:Humans; D053768:Metal Nanoparticles; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence
chemical_list: D006046:Gold; D005492:Folic Acid; D001895:Boron