title: Separation of anti-neoplastic activities by fractionation of a Pluchea odorata extract.
abstract: Natural products continue to represent the main source for therapeutics, and ethnopharmacological remedies from high biodiversity regions are a rich source for the development of novel drugs. Hence, in our attempt to find new anti-neoplastic activities we focused on ethno-medicinal plants of the Maya, who live in the world's third richest area in vascular plant species. Pluchea odorata (Asteraceae) is traditionally used for the treatment of various inflammatory disorders and recently, the in vitro anti-cancer activities of different extracts of this plant were described. Here, we present the results of bioassay-guided fractionations of the dichloromethane extract of P. odorata that aimed to enrich the active principles. The separation resulted in fractions which showed the dissociation of two distinct anti-neoplastic mechanisms; firstly, a genotoxic effect that was accompanied by tubulin polymerization, cell cycle arrest, and apoptosis (fraction F2/11), and secondly, an effect that interfered with the orchestrated expression of Cyclin D1, Cdc25A, and Cdc2 and that also led to cell cycle arrest and apoptosis (fraction F3/4). Thus, the elimination of generally toxic properties and beyond that the development of active principles of P. odorata, which disturb cancer cell cycle progression, are of interest for potential future therapeutic concepts against proliferative diseases.
authors.affiliation: Institute of Clinical Pathology, Medical University of Vienna, Vienna, Austria.
authors.forename: Sabine
authors.initials: S
authors.lastname: Bauer
authors.forename: Judith
authors.initials: J
authors.lastname: Singhuber
authors.forename: Mareike
authors.initials: M
authors.lastname: Seelinger
authors.forename: Christine
authors.initials: C
authors.lastname: Unger
authors.forename: Katharina
authors.initials: K
authors.lastname: Viola
authors.forename: Caroline
authors.initials: C
authors.lastname: Vonach
authors.forename: Benedikt
authors.initials: B
authors.lastname: Giessrigl
authors.forename: Sibylle
authors.initials: S
authors.lastname: Madlener
authors.forename: Nicole
authors.initials: N
authors.lastname: Stark
authors.forename: Bruno
authors.initials: B
authors.lastname: Wallnofer
authors.forename: Karl-Heinz
authors.initials: KH
authors.lastname: Wagner
authors.forename: Monika
authors.initials: M
authors.lastname: Fritzer-Szekeres
authors.forename: Thomas
authors.initials: T
authors.lastname: Szekeres
authors.forename: Rene
authors.initials: R
authors.lastname: Diaz
authors.forename: Foster
authors.initials: F
authors.lastname: Tut
authors.forename: Richard
authors.initials: R
authors.lastname: Frisch
authors.forename: Bjorn
authors.initials: B
authors.lastname: Feistel
authors.forename: Brigitte
authors.initials: B
authors.lastname: Kopp
authors.forename: Georg
authors.initials: G
authors.lastname: Krupitza
authors.forename: Ruxandra
authors.initials: R
authors.lastname: Popescu
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification*; D019659:Asteraceae / Q000737:chemistry*; D015153:Blotting, Western; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D006801:Humans; D010936:Plant Extracts / Q000302:isolation & purification*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts