title: Hypoxia induces beta-amyloid in association with death of RGC-5 cells in culture.
abstract: Beta-amyloid (Aβ) derived from amyloid precursor protein (APP) has been associated with retinal degeneration in Alzheimer's disease (AD) and glaucoma. This study examined whether hypoxia exposure induces Aβ accumulation in RGC-5 cells. While levels of APP mRNA and protein significantly increased in the cells, elevated abundance of Aβ was also observed in cells and culture medium between 12 or 24 and 48h after 5% O(2) hypoxia treatment. Additionally, there is a close relationship between induction of APP and Aβ and intracellular accumulation of ROS along with loss of mitochondrial membrane potential followed by the death of RGC-5 cells in culture under hypoxia. These results suggest a possible involvement of APP and Aβ in the death of RGCs challenged by hypoxia.
authors.affiliation: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.forename: Zhizhang
authors.initials: Z
authors.lastname: Dong
authors.forename: Bingqian
authors.initials: B
authors.lastname: Liu
authors.forename: Yehong
authors.initials: Y
authors.lastname: Zhuo
authors.forename: Xuerong
authors.initials: X
authors.lastname: Sun
authors.forename: Zhikuan
authors.initials: Z
authors.lastname: Yang
authors.forename: Jian
authors.initials: J
authors.lastname: Ge
authors.forename: Zhiqun
authors.initials: Z
authors.lastname: Tan
mesh_terms: D016564:Amyloid beta-Protein Precursor / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D017209:Apoptosis; D018929:Cell Culture Techniques; D002460:Cell Line; D003470:Culture Media / Q000737:chemistry; D000860:Hypoxia / Q000378:metabolism* / Q000473:pathology*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D012162:Retinal Degeneration / Q000378:metabolism* / Q000473:pathology*
chemical_list: D016564:Amyloid beta-Protein Precursor; D003470:Culture Media; D012333:RNA, Messenger