title: The microalga Chlamydomonas reinhardtii as a platform for the production of human protein therapeutics.
abstract: Microalgae are a diverse group of eukaryotic photosynthetic microorganisms. While microalgae play a crucial role in global carbon fixation and oxygen evolution, these organisms have recently gained much attention for their potential role in biotechnological and industrial applications, such as the production of biofuels. We investigated the potential of the microalga Chlamydomonas reinhardtii to be a platform for the production of human therapeutic proteins. C. reinhardtii is a unicellular freshwater green alga that has served as a popular model alga for physiological, molecular, biochemical and genetic studies. As such, the molecular toolkit for this microorganism is highly developed, including well-established methods for genetic transformation and recombinant gene expression. We transformed the chloroplast genome of C. reinhardtii with seven unrelated genes encoding for current or potential human therapeutic proteins and found that four of these genes supported protein accumulation to levels that are sufficient for commercial production. Furthermore, the algal-produced proteins were bioactive. Thus, the microalga C. reinhardtii has the potential to be a robust platform for human therapeutic protein production.
authors.affiliation: Division of Biological Sciences University of California, La Jolla, CA, USA.
authors.forename: Beth A
authors.initials: BA
authors.lastname: Rasala
authors.forename: Stephen P
authors.initials: SP
authors.lastname: Mayfield
mesh_terms: D001709:Biotechnology / Q000379:methods*; D016825:Chlamydomonas reinhardtii / Q000235:genetics / Q000378:metabolism*; D002736:Chloroplasts / Q000235:genetics; D054628:Genome, Chloroplast / Q000235:genetics; D006801:Humans; D008957:Models, Genetic