title: [The associations of methylenetetrahydrofolate reductase gene C677T and A1298C polymorphisms and ulcerative colitis].
abstract: OBJECTIVE
  To investigate the association between the genetic polymorphisms of methylenetetrahydrofolate reductase (MTHFR) and ulcerative colitis (UC) of Han ethnic population in Zhejiang, China.
  
  
  METHODS
  Two hundred and seventy-four consecutive patients with UC and 726 healthy controls (HC) were studied. The genetic polymorphisms of MTHFR (C677T and A1298C) were genotyped using PCR-RELP methods.
  
  
  RESULTS
  The frequencies of variant allele and genotype in MTHFR A1298C gene were higher in UC patients than in the HC (35.77% vs 29.96%, P = 0.013; 52.19% vs 44.90%, P = 0.039; respectively). However, there were no significant discrepancies of the allele and genotype frequencies in the MTHFR C677T gene between the UC patients and the HC (P > 0.05). In addition, the MTHFR 677TT homozygote, T allele and 677CT/1298AC compound genotype were more prevalent in patients with extensive colitis than in those with distal colitis (37.66% vs 14.72%, P = 0.0002; 49.35% vs 32.99%, P = 0.0004; 29.87% vs 15.23%, P = 0.006; respectively). Furthermore, the variant allele in the MTHFR A1298C gene (C) in severe UC patients was significantly lower than in mild and moderate UC patients (18.97% vs 33.88%, P = 0.022).
  
  
  CONCLUSIONS
  The genetic polymorphisms of MTHFR C677T and A1298C are obviously associated with Han ethnic population with UC in Zhejiang province.
authors.affiliation: Department of Gastroenterology, Wenzhou Medical College, Wenzhou 325027, China.
authors.forename: Chang-long
authors.initials: CL
authors.lastname: Xu
authors.forename: Xiu-qing
authors.initials: XQ
authors.lastname: Lin
authors.forename: De-yun
authors.initials: DY
authors.lastname: Lan
authors.forename: Jian-zhang
authors.initials: JZ
authors.lastname: Wang
authors.forename: Bo
authors.initials: B
authors.lastname: Zheng
authors.forename: Zhan-xiong
authors.initials: ZX
authors.lastname: Xue
mesh_terms: D000328:Adult; D000483:Alleles; D044466:Asian People / Q000235:genetics; D016022:Case-Control Studies; D002681:China; D003093:Colitis, Ulcerative / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006720:Homozygote; D006801:Humans; D008297:Male; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D008875:Middle Aged; D011110:Polymorphism, Genetic; D016324:Sequence Tagged Sites
chemical_list: D042965:Methylenetetrahydrofolate Reductase (NADPH2)