title: A new aminopeptidase inhibitor from Streptomyces strain HCCB10043 found by UPLC-MS.
abstract: The diversity of microbial metabolites has been of interest and concern for a long time, yet a suitable method for discovering these is still unavailable. In the work discussed in this report, ultra-performance liquid chromatography coupled with tandem quadrupole and time of flight high-resolution mass spectrometry (UPLC-Q-TOF-HRMS), with MS data analysis, was set up to study the metabolites of Streptomyces strain HCCB10043. It was found that besides antibacterial substances (A21978C complex) and two anti-aminopeptidase compounds (valistatin and bestatin), this strain can produce a new aminopeptidase inhibitor, identified as 3-amino-2-hydroxy-4-phenylbutanoylvalylisoleucine. This new compound had greater activity than valistatin or bestatin in aminopeptidase N (APN) inhibition assay. The results proved that combination of UPLC-Q-TOF-MS analysis and classic purification and identification steps as complementary strategies can provide a method with high reliability for research on microbial secondary metabolites. Furthermore, it has shown that the study of secondary metabolic profiling might be the key to discovering new drugs.
authors.affiliation: College of Life Science, Nanjing Agricultural University, Nanjing, Jiangsu, China. rm1009@126.com
authors.forename: Min
authors.initials: M
authors.lastname: Rao
authors.forename: Qiushuang
authors.initials: Q
authors.lastname: Li
authors.forename: Lei
authors.initials: L
authors.lastname: Feng
authors.forename: Xing
authors.initials: X
authors.lastname: Xia
authors.forename: Lijun
authors.initials: L
authors.lastname: Ruan
authors.forename: Xiafang
authors.initials: X
authors.lastname: Sheng
authors.forename: Mei
authors.initials: M
authors.lastname: Ge
mesh_terms: D018826:CD13 Antigens / Q000037:antagonists & inhibitors; D002853:Chromatography, Liquid; D013058:Mass Spectrometry; D015394:Molecular Structure; D009842:Oligopeptides / Q000737:chemistry* / Q000494:pharmacology; D011480:Protease Inhibitors / Q000737:chemistry* / Q000494:pharmacology; D013302:Streptomyces / Q000737:chemistry* / Q000378:metabolism; D013329:Structure-Activity Relationship
chemical_list: C562326:3-amino-2-hydroxy-4-phenylbutanoylvalylisoleucine; D009842:Oligopeptides; D011480:Protease Inhibitors; D018826:CD13 Antigens