title: Growth promotion of Bifidobacterium species by poultry bone and meat trimming hydrolyzate.
abstract: The growth of bifidobacteria that are employed in the production of functional food is often slow or limited, even on synthetic media. In this study, we investigated whether a peptide hydrolyzate (functional animal protein [FAP]), from poultry bones and meat trimmings, could be a potential source of growth stimulators. The bifidogenic activity of FAP on 18 strains of Bifidobacterium species was assessed via 2 different techniques: turbidimetric measurements and a direct count by fluorescence microscopy. Growth experiments were performed in B12 broth as the basal medium, B12 broth supplemented with N-acetylglucosamine, and B12 broth supplemented with FAP. FAP supplementation yielded the highest maximum optical density (OD) and count values. The use of the microscopic fluorescence counts allowed for better evaluation of the extent of growth and assessment of the viability of cells. FAP from poultry bones and meat trimmings has potential as a growth stimulator for different bifidobacteria of human origin. FAP is a promising ingredient for inclusion in industrial media that are used to culture probiotic strains, including bifidobacteria, because it supports growth very well and maintains cells at a high level of viability.
  
  
  CONCLUSIONS
  Proteinaceous hydrolyzate can be considered a promising ingredient for industrial media that are used to culture probiotic strains, including bifidobacteria, because it improves bacterial growth and maintains cells at a high level of viability.
authors.affiliation: Dept. of Genetics, Biology of Microorganisms, Anthropology and Evolution, Univ. of Parma, Viale Usberti 11/A, 43100 Parma, Italy. camilla.lazzi@unipr.it
authors.forename: Camilla
authors.initials: C
authors.lastname: Lazzi
authors.forename: Federica
authors.initials: F
authors.lastname: Meli
authors.forename: Arnaldo
authors.initials: A
authors.lastname: Dossena
authors.forename: Monica
authors.initials: M
authors.lastname: Gatti
authors.forename: Erasmo
authors.initials: E
authors.lastname: Neviani
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001644:Bifidobacterium / Q000254:growth & development* / Q000302:isolation & purification; D001842:Bone and Bones / Q000378:metabolism*; D015169:Colony Count, Microbial; D003470:Culture Media / Q000191:economics / Q000378:metabolism*; D005060:Europe; D005243:Feces / Q000382:microbiology; D055951:Functional Food / Q000191:economics / Q000382:microbiology; D006801:Humans; D007220:Industrial Waste / Q000032:analysis* / Q000191:economics; D008462:Meat-Packing Industry / Q000191:economics; D050296:Microbial Viability; D008856:Microscopy, Fluorescence; D009391:Nephelometry and Turbidimetry; D011200:Poultry; D011202:Poultry Products / Q000032:analysis*; D019936:Probiotics / Q000191:economics / Q000378:metabolism; D011492:Protein Hydrolysates / Q000737:chemistry / Q000378:metabolism*; D013045:Species Specificity
chemical_list: D000596:Amino Acids; D003470:Culture Media; D007220:Industrial Waste; D011492:Protein Hydrolysates