title: Molecular signature induced by RNASET2, a tumor antagonizing gene, in ovarian cancer cells.
abstract: Using the Hey3Met2 human ovarian cancer cell line, we previously found the RNASET2 gene to possess a remarkable in vivo tumor suppressor activity, although no in vitro features such as inhibition of cell proliferation, clonogenic potential, impaired growth in soft agar and increase in apoptotic rate could be detected. This is reminiscent of the behavior of genes belonging to the class of tumor antagonizing genes (TAG) which act mainly within the context of the microenvironment. Here we present transcriptional profiles analysis which indicates that investigations of the mechanisms of TAG biological functions require a comparison between the in vitro and in vivo expression patterns. Indeed several genes displaying a biological function potentially related to tumor suppression could not be validated by subsequent in vivo expression analysis. On the other hand the fact that we could find congruency for three genes both in vivo and in vitro adds a warning to a too much stringent categorization of this class of genes which relies on the sensitivity of the methodological approaches.
authors.affiliation: Dipartimento di Biotecnologie e Scienze Molecolari, Università degli Studi dell'Insubria, via JH Dunant 3, 21100 Varese, Italy. francesco.acquati@uninsubria.it
authors.forename: Francesco
authors.initials: F
authors.lastname: Acquati
authors.forename: Laura
authors.initials: L
authors.lastname: Monti
authors.forename: Marta
authors.initials: M
authors.lastname: Lualdi
authors.forename: Marco
authors.initials: M
authors.lastname: Fabbri
authors.forename: Maria Grazia
authors.initials: MG
authors.lastname: Sacco
authors.forename: Laura
authors.initials: L
authors.lastname: Gribaldo
authors.forename: Roberto
authors.initials: R
authors.lastname: Taramelli
mesh_terms: D000818:Animals; D002277:Carcinoma / Q000235:genetics* / Q000473:pathology; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D016147:Genes, Tumor Suppressor / Q000502:physiology*; D006801:Humans; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000473:pathology; D012260:Ribonucleases / Q000235:genetics / Q000502:physiology*; D014407:Tumor Cells, Cultured; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000502:physiology*; D015854:Up-Regulation / Q000235:genetics; D054928:Validation Studies as Topic
chemical_list: D025521:Tumor Suppressor Proteins; D012260:Ribonucleases; C425232:RNASET2 protein, human