title: Characterisation of exosomes derived from human cells by nanoparticle tracking analysis and scanning electron microscopy.
abstract: Exosomes from three different cell types (HEK 293T, ECFC, MSC) were characterised by scanning electron microscopy (SEM), dynamic light scattering (DLS) and nanoparticle tracking analysis (NTA). The diameter was around 110 nm for the three cell types. The stability of exosomes was examined during storage at -20°C, 4°C, and 37°C. The size of the exosomes decreased at 4°C and 37°C, indicating a structural change or degradation. Multiple freezing to -20°C and thawing did not affect the exosome size. Multiple ultracentrifugation also did not change the exosome size.
authors.affiliation: Institute of Inorganic Chemistry, Faculty of Chemistry, University of Duisburg-Essen, Essen, Germany.
authors.forename: Viktoriya
authors.initials: V
authors.lastname: Sokolova
authors.forename: Anna-Kristin
authors.initials: AK
authors.lastname: Ludwig
authors.forename: Sandra
authors.initials: S
authors.lastname: Hornung
authors.forename: Olga
authors.initials: O
authors.lastname: Rotan
authors.forename: Peter A
authors.initials: PA
authors.lastname: Horn
authors.forename: Matthias
authors.initials: M
authors.lastname: Epple
authors.forename: Bernd
authors.initials: B
authors.lastname: Giebel
mesh_terms: D003102:Colloids; D042783:Endothelial Cells / Q000166:cytology*; D055354:Exosomes / Q000648:ultrastructure*; D057809:HEK293 Cells; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles / Q000648:ultrastructure*; D036103:Nanotechnology / Q000379:methods*; D010316:Particle Size; D013696:Temperature
chemical_list: D003102:Colloids