title: Label free biochemical 2D and 3D imaging using secondary ion mass spectrometry.
abstract: Time-of-flight secondary ion mass spectrometry (ToF-SIMS) provides a method for the detection of native and exogenous compounds in biological samples on a cellular scale. Through the development of novel ion beams the amount of molecular signal available from the sample surface has been increased. Through the introduction of polyatomic ion beams, particularly C(60), ToF-SIMS can now be used to monitor molecular signals as a function of depth as the sample is eroded thus proving the ability to generate 3D molecular images. Here we describe how this new capability has led to the development of novel instrumentation for 3D molecular imaging while also highlighting the importance of sample preparation and discuss the challenges that still need to be overcome to maximise the impact of the technique.
authors.affiliation: School of Chemical Engineering and Analytical Science, Manchester Interdisciplinary Biocentre, University of Manchester, Manchester M1 7DN, UK. John.Fletcher@manchester.ac.uk
authors.forename: John S
authors.initials: JS
authors.lastname: Fletcher
authors.forename: John C
authors.initials: JC
authors.lastname: Vickerman
authors.forename: Nicholas
authors.initials: N
authors.lastname: Winograd
mesh_terms: D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D002462:Cell Membrane / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000295:instrumentation / Q000379:methods*; D007477:Ions / Q000737:chemistry; D057054:Molecular Imaging / Q000295:instrumentation / Q000379:methods*; D010743:Phospholipids / Q000032:analysis*; D025341:Principal Component Analysis; D012984:Software; D018629:Spectrometry, Mass, Secondary Ion / Q000295:instrumentation / Q000379:methods*; D014982:Xenopus laevis
chemical_list: D000596:Amino Acids; D007477:Ions; D010743:Phospholipids