title: Design and synthesis of biofunctionalized metallic/magnetic nanomaterials.
abstract: Organic solvent-soluble nanocrystals suitable for magnetic resonance imaging are prepared by two routes, namely, a coprecipitation method and a multiple-step thermal decomposition method (seed-mediated growth). The size, shape, crystallinity, phase, and composition of the prepared nanocrystals are determined by various characterization techniques, including transmission electron microscopy, vibrating-sample magnetometer, X-ray diffraction, and inductively coupled plasma mass spectroscopy. Subsequently, the organic-soluble nanocrystals are rendered water-soluble by two methods, the microemulsion method and the ligand exchange method, for biomedical applications. Detailed protocols for the preparation of water-soluble nanocrystals, as well as procedures for drug-loading and antibody conjugation to the water-soluble nanocrystals are provided.
authors.forename: Eun-Kyung
authors.initials: EK
authors.lastname: Lim
authors.forename: Seungjoo
authors.initials: S
authors.lastname: Haam
authors.forename: Kwangyeol
authors.initials: K
authors.lastname: Lee
authors.forename: Yong-Min
authors.initials: YM
authors.lastname: Huh
mesh_terms: D000906:Antibodies / Q000737:chemistry; D011232:Chemical Precipitation; D015195:Drug Design; D004655:Emulsions; D057927:Hydrophobic and Hydrophilic Interactions; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D010316:Particle Size; D011108:Polymers / Q000737:chemistry; D013696:Temperature
chemical_list: D000906:Antibodies; D004655:Emulsions; D058185:Magnetite Nanoparticles; D011108:Polymers