title: Extended-spectrum beta-lactamase-producing Escherichia coli in turkey meat production farms in the Czech Republic: national survey reveals widespread isolates with bla(SHV-12) genes on IncFII plasmids.
abstract: OBJECTIVE
  The occurrence and epidemiology of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli in the environment of turkey farms in the Czech Republic were studied.
  
  
  RESULTS
  Extended-spectrum beta-lactamase-producing E. coli isolates were found on 8 (20%) of 40 turkey farms surveyed. A total of 200 environmental smears were examined, and a total of 25 ESBL-producing E. coli were isolated. These isolates were analysed using XbaI pulsed-field gel electrophoresis and divided into nine pulsotypes. Most of the isolates harboured the gene bla(SHV-12) on a 40-kb plasmid of the IncFII group with an identical EcoRV restriction profile. Indistinguishable or clonally related SHV-12-producing isolates belonging to the same pulsotypes were found at some unrelated farms.
  
  
  CONCLUSIONS
  Widespread occurrence of ESBL-producing E. coli isolates with bla(SHV-12) carried on IncFII plasmids in meat production flocks in the Czech Republic was demonstrated.
  
  
  CONCLUSIONS
  Results indicate vertical transmission of ESBL-producing E. coli within the turkey production pyramid. The study shows the risk of multiresistant ESBL-producing bacteria and antibiotic-resistance genes being transmitted to humans via the food chain.
authors.affiliation: Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackecho, Czech Republic. dolejskam@vfu.cz
authors.forename: M
authors.initials: M
authors.lastname: Dolejska
authors.forename: M
authors.initials: M
authors.lastname: Matulova
authors.forename: L
authors.initials: L
authors.lastname: Kohoutova
authors.forename: I
authors.initials: I
authors.lastname: Literak
authors.forename: J
authors.initials: J
authors.lastname: Bardon
authors.forename: A
authors.initials: A
authors.lastname: Cizek
mesh_terms: D000822:Animal Husbandry; D000818:Animals; D018153:Czech Republic; D016521:Electrophoresis, Gel, Pulsed-Field; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000235:genetics / Q000382:microbiology; D029968:Escherichia coli Proteins / Q000235:genetics; D006801:Humans; D008460:Meat / Q000382:microbiology*; D010957:Plasmids; D016133:Polymerase Chain Reaction; D014422:Turkeys; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D029968:Escherichia coli Proteins; D001618:beta-Lactamases