title: Microbial biosensors for organophosphate pesticides.
abstract: Organophosphates, amongst the most toxic substance known, are used widely in agriculture around the world. Their extensive use, however, has resulted in their occurrence in the water and food supply threatening humans and animals. Therefore, there is a need for determination of these neurotoxic compounds sensitively, selectively, and rapidly in the field. The present work is a brief review on the recent advancements in amperometric, potentiometric, and optical biosensors using genetically engineered microorganisms expressing organophosphate hydrolyzing enzyme intracellularly or anchored on the cell surface for the detection of organophosphate pesticides. The benefits and limitations associated with such microbial biosensors are delineated.
authors.affiliation: Department of Chemical and Environmental Engineering, University of California, Riverside, CA 92521, USA. adani@engr.ucr.edu
authors.forename: Ashok
authors.initials: A
authors.lastname: Mulchandani
authors.lastname: Rajesh
mesh_terms: D000818:Animals; D043303:Aryldialkylphosphatase / Q000737:chemistry / Q000378:metabolism*; D015374:Biosensing Techniques / Q000379:methods*; D004566:Electrodes; D004926:Escherichia coli / Q000737:chemistry / Q000378:metabolism; D005417:Flavobacterium / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007306:Insecticides / Q000032:analysis*; D009943:Organophosphorus Compounds / Q000032:analysis*; D010575:Pesticides / Q000032:analysis*; D011199:Potentiometry / Q000379:methods*; D016958:Pseudomonas putida / Q000737:chemistry / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000378:metabolism
chemical_list: D007306:Insecticides; D009943:Organophosphorus Compounds; D010575:Pesticides; D043303:Aryldialkylphosphatase