title: Cytogenetic characterization and mapping of rDNAs, core histone genes and telomeric sequences in Venerupis aurea and Tapes rhomboides (Bivalvia: Veneridae).
abstract: We describe the chromosomal location of GC-rich regions, 28S and 5S rDNA, core histone genes, and telomeric sequences in the veneroid bivalve species Venerupis aurea and Tapes (Venerupis) rhomboides, using fluorochrome staining with propidium iodide, DAPI and chromomycin A3 (CMA) and fluorescent in situ hybridization (FISH). DAPI dull/CMA bright bands were coincident with the chromosomal location of 28S rDNA in both species. The major rDNA was interstitially clustered at a single locus on the short arms of the metacentric chromosome pair 5 in V. aurea, whereas in T. rhomboides it was subtelomerically clustered on the long arms of the subtelocentric chromosome pair 17. 5S rDNA also was a single subtelomeric cluster on the long arms of subtelocentric pair 17 in V. aurea and on the short arms of the metacentric pair 9 in T. rhomboides. Furthermore, V. aurea showed four telomeric histone gene clusters on three metacentric pairs, at both ends of chromosome 2 and on the long arms of chromosomes 3 and 8, whereas histone genes in T. rhomboides clustered interstitially on the long arms of the metacentric pair 5 and proximally on the long arms of the subtelocentric pair 12. Double and triple FISH experiments demonstrated that rDNA and H3 histone genes localized on different chromosome pairs in the two clam species. Telomeric signals were found at both ends of every single chromosome in both species. Chromosomal location of these three gene families in two species of Veneridae provides a clue to karyotype evolution in this commercially important bivalve family.
authors.affiliation: Department of Biochemistry, Genetics and Immunology, University of Vigo, 36310 Vigo, Spain.
authors.forename: Joana
authors.initials: J
authors.lastname: Carrilho
authors.forename: Concepción
authors.initials: C
authors.lastname: Pérez-García
authors.forename: Alexandra
authors.initials: A
authors.lastname: Leitão
authors.forename: Isabel
authors.initials: I
authors.lastname: Malheiro
authors.forename: Juan J
authors.initials: JJ
authors.lastname: Pasantes
mesh_terms: D000818:Animals; D001482:Base Composition / Q000235:genetics; D049872:Bivalvia / Q000235:genetics*; D002874:Chromosome Mapping; D020732:Cytogenetic Analysis; D004275:DNA, Ribosomal / Q000235:genetics*; D005260:Female; D006657:Histones / Q000235:genetics*; D008297:Male; D016615:Telomere / Q000235:genetics*
chemical_list: D004275:DNA, Ribosomal; D006657:Histones