title: PinX1 gene transfection enhances the sensitivity of gastric carcinoma cell line to 5-fluorouracil.
abstract: OBJECTIVE
  Since telomeres and telomerase play crucial roles in maintaining cell immortalization and cancer progression, they may be targets for anticancer treatment. PinX1 is a potent telomerase inhibitor, and a putative tumor suppressor. The use of PinX1 to treat cancers has not been reported yet.
  
  
  METHODS
  In order to use PinX1 in gene therapy for gastric carcinoma, we transfected PinX1 gene into the gastric carcinoma line MKN28 using the eukaryotic expression vector pIRES2-EGFP. PinX1-expressing, drug-resistant clones were screened with G418 and used in the study.
  
  
  RESULTS
  MKN28 cells transfected with PinX1 gene grew more slowly than the cells not transfected or transfected with void vectors (p<0.05). The IC50 value decreased markedly in cells transfected with PinX1 gene. PinX1 gene transfection enhanced the sensitivity of MKN28 cells to 5-fluorouracil (p<0.05).
  
  
  CONCLUSIONS
  PinX1 may be used in gene therapy for gastric carcinoma.
authors.affiliation: Institute of Gastroenterology, Southwest Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Hong-Bin
authors.initials: HB
authors.lastname: Wang
authors.forename: Wei-Qiang
authors.initials: WQ
authors.lastname: Wang
authors.forename: Xing-Wei
authors.initials: XW
authors.lastname: Wang
authors.forename: Yong-Gang
authors.initials: YG
authors.lastname: Sun
authors.forename: Gang
authors.initials: G
authors.lastname: Zhou
authors.forename: Shi-Ming
authors.initials: SM
authors.lastname: Yang
authors.forename: Dian-Chun
authors.initials: DC
authors.lastname: Fan
mesh_terms: D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology*; D018797:Cell Cycle Proteins; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005472:Fluorouracil / Q000494:pharmacology*; D015316:Genetic Therapy; D006801:Humans; D013274:Stomach Neoplasms / Q000473:pathology / Q000628:therapy*; D019098:Telomerase / Q000502:physiology; D014162:Transfection; D025521:Tumor Suppressor Proteins / Q000235:genetics*
chemical_list: D000964:Antimetabolites, Antineoplastic; D018797:Cell Cycle Proteins; C440808:PINX1 protein, human; D025521:Tumor Suppressor Proteins; C509186:TERT protein, human; D019098:Telomerase; D005472:Fluorouracil