title: Effect of bioactive ceramic and recombinant human transforming growth factor-beta (rhTGF-beta) on regeneration of parietal bone defects in rat.
abstract: OBJECTIVE
  The purpose of this study was to evaluate the regenerative response of parietal bone defects in rats to bioactive ceramic and TGF-beta 1.
  
  
  METHODS
  Sixty mature adult rats with initial weights of 300 g to 324 g were used. The diameter of each defect was 3 mm. The animals were divided into four study groups consisting of 15 rats per group. Groups 1 and 3 (control groups) received 30 μg bioceramic per surgical defect site. Groups 2 and 4 (experimental groups) received 30 μg bioceramic combined with 10 μL of reconstituted TGF-beta 1 (200 ng). Animals of Groups 1 and 3 were euthanized at 18 days; Group 2 and 4 animals were terminated at 49 days after surgery. Each of the outcomes was analyzed via two-way analysis of variance model (ANOVA).
  
  
  RESULTS
  Histologic reactions to the implants were similar with no significant difference between bioceramic and bioceramic + TGF-beta 1 specimens. In the 18-day specimens, the percentage of ceramic was marginally higher than the 49-day specimens. No significant difference was noted in the percentage of bone in 18-day and 49-day specimens.
  
  
  CONCLUSIONS
  Based on the findings of this study, it was concluded that TGF-beta 1 promoted no significant increase in new bone when compared to bioactive ceramic alone.
authors.affiliation: Periodontics Department, Dental School, Shahid Beheshti University of Medical Sciences, Daneshju Blvd., Tehran, Iran.
authors.forename: Fariborz
authors.initials: F
authors.lastname: Nasseri
authors.forename: Gholam Ali
authors.initials: GA
authors.lastname: Gholami
authors.forename: Mahdi
authors.initials: M
authors.lastname: Kadkhodazadeh
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects*; D002516:Ceramics; D048091:Guided Tissue Regeneration; D008297:Male; D010294:Parietal Bone / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000494:pharmacology; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D011994:Recombinant Proteins; D053773:Transforming Growth Factor beta1