title: Phosphoproteins and the dawn of functional phenotyping.
abstract: Phosphorylation is one of the most important processes in cell signal transduction. Detection of phosphorylated proteins in cancer tissue is useful for prognosis and diagnosis, and it might be very helpful in monitoring treatment using targeted therapy. For these reasons, the in situ quantitative measurement and subcellular localization of phosphoproteins will likely be important. However, phosphoproteins are extremely labile, a likely explanation for inconsistent or contradictory reports. Thus, the development of new paradigms for tissue handling, immunostaining, and quality control are needed.
authors.affiliation: Department of Clinical Pathology, Cleveland Clinic, OH 44195, USA.
authors.forename: J
authors.initials: J
authors.lastname: Bodo
authors.forename: E D
authors.initials: ED
authors.lastname: Hsi
mesh_terms: D000918:Antibody Specificity; D005455:Fluorescent Antibody Technique; D006801:Humans; D007150:Immunohistochemistry; D058990:Molecular Targeted Therapy; D009369:Neoplasms / Q000175:diagnosis* / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism*; D010641:Phenotype; D010750:Phosphoproteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D011379:Prognosis; D040901:Proteomics; D011786:Quality Control; D045663:Quantum Dots; D015398:Signal Transduction; D013048:Specimen Handling
chemical_list: D010750:Phosphoproteins