title: Induced bone marrow mesenchymal stem cells improve cardiac performance of infarcted rat hearts.
abstract: We investigated whether transplantation of bone marrow mesenchymal stem cells (BMSC) with induced BMSC (iBMSC) or uninduced BMSC (uBMSC) into the myocardium could improve the performance of post-infarcted rat hearts. BMSCs were specified by flowcytometry. IBMSCs were cocultured with rat cardiomyocyte before transplantation. Cells were injected into borders of cardiac scar tissue 1 week after experimental infarction. Cardiac performance was evaluated by echocardiography at 1, 2, and 4 weeks after cellular or PBS injection. Langendorff working-heart and histological studies were performed 4 weeks after treatment. Myogenesis was detected by quantitative PCR and immunofluorescence. Echocardiography showed a nearly normal ejection fraction (EF) in iBMSC-treated rats and all sham control rats but a lower EF in all PBS-treated animals. The iBMSC-treated heart, assessed by echocardiography, improved fractional shortening compared with PBS-treated hearts. The coronary flow (CF) was decreased obviously in PBS and uBMSC-treated groups, but recovered in iBMSC-treated heart at 4 weeks (P < 0.01). Immunofluorescent microscopy revealed co-localization of Superparamagnetic iron oxide (SPIO)-labeled transplanted cells with cardiac markers for cardiomyocytes, indicating regeneration of damaged myocardium. These data provide strong evidence that iBMSC implantation is of more potential to improve infarcted cardiac performance than uBMSC treatment. It will open new promising therapeutic opportunities for patients with post-infarction heart failure.
authors.affiliation: Medical Research Center, Guangdong General Hospital, Guangdong Provincial Cardiovascular Institute, Guangdong Academy of Medical Sciences, 96 Dongchuan Road, Weilun Bldg, Guangzhou 510080, People's Republic of China.
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Li
authors.forename: Yong-Heng
authors.initials: YH
authors.lastname: Fu
authors.forename: Qiu-Xiong
authors.initials: QX
authors.lastname: Lin
authors.forename: Zai-Yi
authors.initials: ZY
authors.lastname: Liu
authors.forename: Zhi-Xin
authors.initials: ZX
authors.lastname: Shan
authors.forename: Chun-Yu
authors.initials: CY
authors.lastname: Deng
authors.forename: Jie-Ning
authors.initials: JN
authors.lastname: Zhu
authors.forename: Min
authors.initials: M
authors.lastname: Yang
authors.forename: Shu-Guang
authors.initials: SG
authors.lastname: Lin
authors.forename: Yangxin
authors.initials: Y
authors.lastname: Li
authors.forename: Xi-Yong
authors.initials: XY
authors.lastname: Yu
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation; D002454:Cell Differentiation / Q000502:physiology; D017931:DNA Primers / Q000235:genetics; D004452:Echocardiography; D005434:Flow Cytometry; D006321:Heart / Q000502:physiology*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D008856:Microscopy, Fluorescence; D024510:Muscle Development / Q000502:physiology; D009203:Myocardial Infarction / Q000628:therapy*; D032383:Myocytes, Cardiac / Q000637:transplantation; D016133:Polymerase Chain Reaction; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D017931:DNA Primers