title: Toxicologic screening of some surfactants using modern in vivo bioassays.
abstract: This paper aims to evaluate the degree of skin irritation using specific in vivo tests. The completion of the study is to develop models with wide applicability in toxicological area. HET-CAM or chorioallantoic membrane assay is a new method accepted as an INVITTOX protocol that is a substitute of Draize test. The methods applied in present study were CAM assay on embryonated egg and CD1 Nu/Nu experimental model. The evaluation of erythema that is an important toxic effect of surfactants was done using a Mexameter MX18 (Courage Khazaka research line). The main observations were that sodium lauryl sulphate is the most toxic compound on our series but the non-ionic surfactants are not completely non-noxious. Non-invasive methods can be associated with other test such as CAM assay to evaluate irritant compounds.
authors.affiliation: School of Pharmacy, Gr.T. Popa University of Medicine and Pharmacy Iaşi.
authors.forename: Simona
authors.initials: S
authors.lastname: Ardelean
authors.forename: Stefana
authors.initials: S
authors.lastname: Feflea
authors.forename: Daniela
authors.initials: D
authors.lastname: Ionescu
authors.forename: V
authors.initials: V
authors.lastname: Năstase
authors.forename: Cristina A
authors.initials: CA
authors.lastname: Dehelean
mesh_terms: D000826:Animal Testing Alternatives; D000818:Animals; D001681:Biological Assay / Q000379:methods; D002642:Chick Embryo; D049033:Chorioallantoic Membrane / Q000187:drug effects*; D004890:Erythema / Q000139:chemically induced; D005123:Eye / Q000187:drug effects; D007509:Irritants / Q000633:toxicity*; D008432:Mathematical Computing; D051379:Mice; D012867:Skin / Q000187:drug effects; D012967:Sodium Dodecyl Sulfate / Q000633:toxicity*; D013501:Surface-Active Agents / Q000633:toxicity*; D018675:Toxicity Tests
chemical_list: D007509:Irritants; D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate