title: FXYD6, a Na,K-ATPase regulator, is expressed in type II taste cells.
abstract: Taste buds contain three types of taste cells. Each type can respond to taste stimulation, and type II and III taste cells are electrically excitable. However, there are differences between the properties of type II and III taste cells. In this study, we found that Fxyd6, an Na,K-ATPase regulator gene, is expressed in type II taste cells in the taste buds of mice. Double-labeled in situ hybridization analysis showed that Fxyd6 was coexpressed with transient receptor potential cation channel, subfamily M, member 5 (Trpm5), a critical component of the sweet, bitter, and umami taste signal transduction pathways and that it was specifically expressed in type II taste cells. We also found that taste cells frequently coexpressed Fxyd6 and Na,K-ATPase β1. These results indicate the presence of an inherent mechanism that regulated transmembrane Na(+) dynamics in type II taste cells.
authors.affiliation: Research Laboratories for Health and Gustatory Science, Asahi Breweries, Ltd., Midori, Moriya, Japan.
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Shindo
authors.forename: Kana
authors.initials: K
authors.lastname: Morishita
authors.forename: Eiichi
authors.initials: E
authors.lastname: Kotake
authors.forename: Hirohito
authors.initials: H
authors.lastname: Miura
authors.forename: Piero
authors.initials: P
authors.lastname: Carninci
authors.forename: Jun
authors.initials: J
authors.lastname: Kawai
authors.forename: Yoshihide
authors.initials: Y
authors.lastname: Hayashizaki
authors.forename: Akihiro
authors.initials: A
authors.lastname: Hino
authors.forename: Tomomasa
authors.initials: T
authors.lastname: Kanda
authors.forename: Yuko
authors.initials: Y
authors.lastname: Kusakabe
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D015870:Gene Expression; D017403:In Situ Hybridization; D007473:Ion Channels / Q000235:genetics / Q000378:metabolism*; D017136:Ion Transport; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000502:physiology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics / Q000378:metabolism*; D050053:TRPM Cation Channels / Q000235:genetics / Q000378:metabolism*; D013649:Taste / Q000502:physiology*; D013650:Taste Buds / Q000166:cytology / Q000201:enzymology*
chemical_list: C518765:FXYD6 protein, mouse; D007473:Ion Channels; D007527:Isoenzymes; D050053:TRPM Cation Channels; C498617:Trpm5 protein, mouse; C497344:Atp1a1 protein, mouse; D000254:Sodium-Potassium-Exchanging ATPase