title: Host cell protein C9orf69 promotes viral proliferation via interaction with HSV-1 UL25 protein.
abstract: In light of the scarcity of reports on the interaction between HSV-1 nucleocapsid protein UL25 and its host cell proteins, the purpose of this study is to use yeast two-hybrid screening to search for cellular proteins that can interact with the UL25 protein. C9orf69, a protein of unknown function was identified. The interaction between the two proteins under physiological conditions was also confirmed by biological experiments including co-localization by fluorescence and immunoprecipitation. A preliminary study of the function of C9orf69 showed that it promotes viral proliferation. Further studies showed that C9orf69 did not influence viral multiplication efficiency by transcriptional regulation of viral genes, but indirectly promoted proliferation via interaction with UL25.
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medicine Science & Peking Union Medical College, Kunming, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yan-Mei
authors.initials: YM
authors.lastname: Li
authors.forename: Long-Ding
authors.initials: LD
authors.lastname: Liu
authors.forename: Li
authors.initials: L
authors.lastname: Jiang
authors.forename: Ma
authors.initials: M
authors.lastname: Ji
authors.forename: Rui-Ju
authors.initials: RJ
authors.lastname: Jiang
authors.forename: Lei
authors.initials: L
authors.lastname: Guo
authors.forename: Yun
authors.initials: Y
authors.lastname: Liao
authors.forename: Qi-Han
authors.initials: QH
authors.lastname: Li
mesh_terms: D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D006561:Herpes Simplex / Q000235:genetics / Q000378:metabolism* / Q000821:virology*; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000502:physiology*; D006801:Humans; D011485:Protein Binding; D011506:Proteins; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D019065:Virus Assembly; D014779:Virus Replication
chemical_list: D002352:Carrier Proteins; D011506:Proteins; C561278:SPACA9 protein, human; C086267:UL24 protein, Human herpesvirus 1; D014764:Viral Proteins