title: Transforming activity of a novel mutant of HPV16 E6E7 fusion gene.
abstract: An optimized recombinant HPV16 E6E7 fusion gene (HPV16 ofE6E7) was constructed according to codon usage for mammalian cell expression, and a mutant of HPV16 ofE6E7 fusion gene (HPV16 omfE6E7) was generated by site-directed mutagenesis at L57G, C113R for the E6 protein and C24G, E26G for the E7 protein for HPV16 ofE6E7 [patent pending (CN 101100672)]. The HPV16 omfE6E7 gene constructed in this work not only lost the transformation capability to NIH 3T3 cells and tumorigenicity in SCID mice, but also maintained very good stability and antigenicity. These results suggests that the HPV16 omfE6E7 gene should undergo further study for application as a safe antigen-specific therapeutic vaccine for HPV16-associated tumors.
authors.affiliation: College of Life Science and Bioengineering, Beijing University of Technology, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Xie
authors.forename: Zhi-Xiang
authors.initials: ZX
authors.lastname: Zhou
authors.forename: Ze-Lin
authors.initials: ZL
authors.lastname: Li
authors.forename: Yi
authors.initials: Y
authors.lastname: Zeng
mesh_terms: D000818:Animals; D002472:Cell Transformation, Viral; D052162:Human papillomavirus 16 / Q000235:genetics / Q000502:physiology*; D006801:Humans; D051379:Mice; D016513:Mice, SCID; D009154:Mutation; D041681:NIH 3T3 Cells; D009856:Oncogene Proteins, Viral / Q000235:genetics* / Q000378:metabolism*; D050725:Papillomavirus E7 Proteins / Q000235:genetics* / Q000378:metabolism*; D030361:Papillomavirus Infections / Q000821:virology; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism*
chemical_list: C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; C059731:oncogene protein E7, Human papillomavirus type 16