title: Characterization of 2-octenoyl-CoA carboxylase/reductase utilizing pteB from Streptomyce avermitilis.
abstract: The filipin biosynthetic gene cluster of Streptomyces avermitilis contains pteB, a homolog of crotonyl-CoA carboxylase/reductase. PteB was predicted to be 2-octenoyl-CoA carboxylase/reductase, supplying hexylmalonyl-CoA to filipin biosynthesis. Recombinant PteB displayed selective reductase activity toward 2-octenoyl-CoA while generating a broad range of alkylmalonyl-CoAs in the presence of bicarbonate.
authors.affiliation: Department of Biological Science, Division of Bioscience and Bioinformatics, Myongji University, Yongin, Republic of Korea.
authors.forename: Hye-Gyeong
authors.initials: HG
authors.lastname: Yoo
authors.forename: So-Yeon
authors.initials: SY
authors.lastname: Kwon
authors.forename: Suji
authors.initials: S
authors.lastname: Kim
authors.forename: Suman
authors.initials: S
authors.lastname: Karki
authors.forename: Zee-Yong
authors.initials: ZY
authors.lastname: Park
authors.forename: Hyung-Jin
authors.initials: HJ
authors.lastname: Kwon
mesh_terms: D000214:Acyl Coenzyme A / Q000378:metabolism*; D044944:Acyl-CoA Dehydrogenases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli; D005372:Filipin / Q000096:biosynthesis; D005810:Multigene Family; D010957:Plasmids; D017386:Sequence Homology, Amino Acid; D013053:Spectrophotometry; D013302:Streptomyces / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D014169:Transformation, Bacterial
chemical_list: D000214:Acyl Coenzyme A; D000900:Anti-Bacterial Agents; C094936:octenoyl-coenzyme A; D005372:Filipin; C010701:crotonyl-coenzyme A; D044944:Acyl-CoA Dehydrogenases; C100154:acyl-CoA dehydrogenase (NADP+)