title: Lack of compartmentalization of HIV-1 quasispecies between the gut and peripheral blood compartments.
abstract: Compartmental differences in human immunodeficiency virus type 1 (HIV-1) between the gut and peripheral blood and within the gut were examined. Biopsy specimens from the colon and ileum and peripheral blood samples were collected from chronically HIV-1-infected individuals. HIV-1 envelope sequences were examined from cell-associated DNA and RNA and virion RNA. Phylogenetic analysis revealed no evidence of compartmentalization of HIV-1 between the gut and peripheral blood and within the gut (colon and ileum). HIV-1 sequences detected in the gut were transcriptionally active and were also found in peripheral blood from matching time points, providing evidence of ongoing virus production in the gut and equilibrium of HIV-1 between the gut and peripheral blood compartments.
authors.affiliation: Clinical and Molecular Retrovirology Section, Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.
authors.forename: Hiromi
authors.initials: H
authors.lastname: Imamichi
authors.forename: Gerald
authors.initials: G
authors.lastname: Degray
authors.forename: Robin L
authors.initials: RL
authors.lastname: Dewar
authors.forename: Peter
authors.initials: P
authors.lastname: Mannon
authors.forename: Michael
authors.initials: M
authors.lastname: Yao
authors.forename: Cheryl
authors.initials: C
authors.lastname: Chairez
authors.forename: Irini
authors.initials: I
authors.lastname: Sereti
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Kovacs
mesh_terms: D001706:Biopsy; D001769:Blood / Q000821:virology*; D003106:Colon / Q000821:virology*; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D005838:Genotype; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007082:Ileum / Q000821:virology*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D054299:env Gene Products, Human Immunodeficiency Virus / Q000235:genetics
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral; D054299:env Gene Products, Human Immunodeficiency Virus