title: Evaluation of IGF2 imprinting status and the levels of folate biomarkers in second trimester pregnant Chinese women.
abstract: OBJECTIVE
  To assess the relationship between folate biomarkers levels and IGF2 imprinting status among second trimester pregnant Chinese women.
  
  
  METHODS
  Three hundred women in their second trimester were screened by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) for analysis of IGF2 imprinting status. Statistical differences of folate biomarkers levels were calculated in the groups with different imprinting status.
  
  
  RESULTS
  Of the 300 women analyzed, 133 (44.33%) cases were homozygous allele with A, 3 (1.00%) cases were homozygous allele with G, while 164 (54.67%) cases were heterozygous with A and G, qualifying them for analysis of loss of imprinting (LOI). Among the 164 cases undergoing LOI analysis, 44 (26.83%) were IGF2 LOI cases, while 120 (73.17%) were IGF2 retention of imprinting (ROI) ones. The mean level of serum folate, vitamin B12 and tHcy was 28.46 +/- 10.74 ng/mL, 380.20 +/- 206.13 pg/mL, 14.24 +/- 6.34 micromol/L among women with IGF2 ROI, and 30.89 +/- 9.97 ng/mL, 394.28 +/- 195.92 pg/ mL, and 13.12 +/- 6.23 micromol/L among women with IGF2 LOI, respectively.
  
  
  CONCLUSIONS
  No significant difference of folate biomarkers levels was observed between IGF2 ROI and IGF2 LOI groups.
authors.affiliation: Laboratory of Reproductive Biology, Chongqing Medical University, Chongqing, People's Republic of China.
authors.forename: Ru-Fei
authors.initials: RF
authors.lastname: Gao
authors.forename: Jun-Lin
authors.initials: JL
authors.lastname: He
authors.forename: Xue-Qing
authors.initials: XQ
authors.lastname: Liu
authors.forename: Yu-Bin
authors.initials: YB
authors.lastname: Ding
authors.forename: Xue-Mei
authors.initials: XM
authors.lastname: Chen
authors.forename: Qian
authors.initials: Q
authors.lastname: Chen
authors.forename: Yi
authors.initials: Y
authors.lastname: Tan
authors.forename: Ying-Xiong
authors.initials: YX
authors.lastname: Wang
mesh_terms: D000328:Adult; D044466:Asian People / Q000235:genetics; D015415:Biomarkers / Q000097:blood; D002681:China; D005260:Female; D005492:Folic Acid / Q000097:blood*; D018392:Genomic Imprinting; D005838:Genotype; D006579:Heterozygote; D006710:Homocysteine / Q000097:blood*; D006720:Homozygote; D006801:Humans; D007335:Insulin-Like Growth Factor II / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011247:Pregnancy; D011262:Pregnancy Trimester, Second; D014805:Vitamin B 12 / Q000097:blood*; D055815:Young Adult
chemical_list: D015415:Biomarkers; D006710:Homocysteine; D007335:Insulin-Like Growth Factor II; D005492:Folic Acid; D014805:Vitamin B 12