title: Using microcontact printing of fibrinogen to control surface-induced platelet adhesion and activation.
abstract: The ability to promote or inhibit specific platelet-surface interactions in well-controlled environments is crucial to studying fundamental adhesion and activation mechanisms. Here, microcontact printing was used to immobilize human fibrinogen covalently in the form of randomly placed, micrometer-sized islands at an overall surface coverage of 20, 50, or 85%. The nonprinted background region was blocked with covalently immobilized human albumin. Platelet adhesion and morphology on each substrate were assessed using combined differential interference and fluorescence microscopy. At 20% coverage, most of the fibrinogen surface features were small round islands, and platelet adhesion and spreading areas were limited by the position and the size of the islands. Platelet circularity, indicated the morphology was mostly rounded. At 50% coverage, some fibrinogen islands coalesced and platelet adhesion and spreading areas increased. Platelet morphology was controlled by the shape of underlying fibrinogen islands, leading to more irregular spreading. At 85% coverage, the fibrinogen pattern was completely interconnected and both platelet adhesion and the spreading area were significantly higher than at lower coverage. In addition, platelets also spread over the albumin regions, suggesting that after a critical surface density of fibrinogen ligands is reached, platelet spreading is no longer inhibited by albumin. Increasing the overall fibrinogen coverage resulted in higher activation levels defined by key morphological characteristics of the spreading platelet.
authors.affiliation: Department of Bioengineering, University of Utah, 20 South 2030 East, Salt Lake City, Utah 84112, USA.
authors.forename: Lindsey E
authors.initials: LE
authors.lastname: Corum
authors.forename: Colin D
authors.initials: CD
authors.lastname: Eichinger
authors.forename: Tony W
authors.initials: TW
authors.lastname: Hsiao
authors.forename: Vladimir
authors.initials: V
authors.lastname: Hlady
mesh_terms: D005340:Fibrinogen / Q000737:chemistry*; D006801:Humans; D010316:Particle Size; D015539:Platelet Activation; D010973:Platelet Adhesiveness; D013499:Surface Properties
chemical_list: D005340:Fibrinogen