title: Loss of Desmocollin 3 in skin tumor development and progression.
abstract: Desmocollin 3 (DSC3) is a desmosomal cadherin that is required for maintaining cell adhesion in the epidermis as demonstrated by the intra-epidermal blistering observed in Dsc3 null skin. Recently, it has been suggested that deregulated expression of DSC3 occurs in certain human tumor types. It is not clear whether DSC3 plays a role in the development or progression of cancers arising in stratified epithelia such as the epidermis. To address this issue, we generated a mouse model in which Dsc3 expression is ablated in K-Ras oncogene-induced skin tumors. Our results demonstrate that loss of Dsc3 leads to an increase in K-Ras-induced skin tumors. We hypothesize that acantholysis-induced epidermal hyperplasia in the Dsc3 null epidermis facilitates Ras-induced tumor development. Further, we demonstrate that spontaneous loss of DSC3 expression is a common occurrence during human and mouse skin tumor progression. This loss occurs in tumor cells invading the dermis. Interestingly, other desmosomal proteins are still expressed in tumor cells that lack DSC3, suggesting a specific function of DSC3 loss in tumor progression. While loss of DSC3 on the skin surface leads to epidermal blistering, it does not appear to induce loss of cell-cell adhesion in tumor cells invading the dermis, most likely due to a protection of these cells within the dermis from mechanical stress. We thus hypothesize that DSC3 can contribute to the progression of tumors both by cell adhesion-dependent (skin surface) and likely by cell adhesion-independent (invading tumor cells) mechanisms.
authors.affiliation: Department of Dermatology, University of Colorado Denver, Aurora, CO 80045, USA.
authors.forename: Jiangli
authors.initials: J
authors.lastname: Chen
authors.forename: Charlene
authors.initials: C
authors.lastname: O'Shea
authors.forename: James E
authors.initials: JE
authors.lastname: Fitzpatrick
authors.forename: Maranke I
authors.initials: MI
authors.lastname: Koster
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Koch
mesh_terms: D000483:Alleles; D000818:Animals; D014408:Biomarkers, Tumor / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology; D051187:Desmocollins; D018450:Disease Progression; D011905:Genes, ras; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics*; D051379:Mice; D012878:Skin Neoplasms / Q000235:genetics / Q000473:pathology*
chemical_list: D014408:Biomarkers, Tumor; D051187:Desmocollins; D008562:Membrane Glycoproteins; C090171:Dsc3 protein, mouse