title: Genetic and biological characterization of a densovirus isolate that affects dengue virus infection.
abstract: Brevidensoviruses have an encapsidated, single-stranded DNA genome that predominantly has a negative polarity. In recent years, they have received particular attention due to their potential role in the biological control of pathogenic arboviruses and to their unnoticed presence in cell cultures as contaminants. In addition, brevidensoviruses may also be useful as viral vectors. This study describes the first genetic and biological characterization of a mosquito densovirus that was isolated in Brazil; moreover, we examined the phylogenetic relationship between this isolate and the other brevidensoviruses. We further demonstrate that this densovirus has the potential to be used to biologically control dengue virus (DENV) infection with in vitro co-infection experiments. The present study provides evidence that this densovirus isolate is a fast-spreading virus that affects cell growth and DENV infection.
authors.affiliation: Instituto Carlos Chagas-Fiocruz, Curitiba, PR, Brasil.
authors.forename: Ana Luiza Pamplona
authors.initials: AL
authors.lastname: Mosimann
authors.forename: Juliano
authors.initials: J
authors.lastname: Bordignon
authors.forename: Giovanny Camacho Antevêre
authors.initials: GC
authors.lastname: Mazzarotto
authors.forename: Maria Cristina M
authors.initials: MC
authors.lastname: Motta
authors.forename: Federico
authors.initials: F
authors.lastname: Hoffmann
authors.forename: Claudia Nunes Duarte Dos
authors.initials: CN
authors.lastname: Santos
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D003716:Dengue Virus / Q000254:growth & development*; D017990:Densovirus / Q000235:genetics* / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D010572:Pest Control, Biological; D010802:Phylogeny