title: Association of inhibitor of differentiation 1 expression with human papillomaviruses infections in cervical carcinoma.
abstract: BACKGROUND
  Our recent study has shown that inhibitor of differentiation 1 (Id-1) is overexpressed in cervical carcinoma. However, the relationship between Id-1 expression and human papillomavirus (HPV) infection has not been elucidated. In this study, we investigate the association of Id-1 protein expression and HPV infection in cervical carcinoma tissues.
  
  
  METHODS
  A total of 56 paraffin-embedded and 12 fresh cervical carcinoma tissues were collected for Id-1 and HPV detection. The Id-1 protein was detected by immunohistochemistry and Western blot in paraffin-embedded and fresh carcinoma tissues. Human papillomavirus DNA was detected and genotyped by using an oligonucleotide microarray and polymerase chain reaction.
  
  
  RESULTS
  The overall HPV prevalence was 82.1%, whereas that of HPV type 16 (HPV-16) was 62.5% in cervical carcinoma. The HPV-positive samples showed higher Id-1 expression levels than the HPV-negative ones (Cochran-Mantel-Haenszel χ test [χCMH] = 4.39, P < 0.05). The HPV-16-infected samples had higher Id-1 expression levels than the samples with infection of other single HPV genotypes (χCMH = 6.42, P < 0.02). The results of Western blot were correlated to the immunohistochemistry results, showing a higher Id-1 expression level in HPV-infected especially HPV-16-infected carcinoma tissues.
  
  
  CONCLUSIONS
  Inhibitor of differentiation 1 expression is correlated to HPV infection in cervical carcinoma, suggesting that Id-1 plays a role in HPV-related cervical carcinogenesis.
authors.affiliation: Department of Gynecology and Obstetrics, West China Second Hospital, Sichuan University, Chengdu, China.
authors.forename: Jinke
authors.initials: J
authors.lastname: Li
authors.forename: Lingxia
authors.initials: L
authors.lastname: Xie
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Gan
authors.forename: Bao
authors.initials: B
authors.lastname: Liu
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Bo
authors.initials: B
authors.lastname: Song
authors.forename: Yong
authors.initials: Y
authors.lastname: Lin
authors.forename: Lina
authors.initials: L
authors.lastname: Hu
mesh_terms: D000328:Adult; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000821:virology; D005260:Female; D005838:Genotype; D052162:Human papillomavirus 16 / Q000235:genetics; D006801:Humans; D051798:Inhibitor of Differentiation Protein 1 / Q000378:metabolism*; D008875:Middle Aged; D030361:Papillomavirus Infections / Q000150:complications / Q000378:metabolism*; D002583:Uterine Cervical Neoplasms / Q000378:metabolism* / Q000821:virology
chemical_list: D051798:Inhibitor of Differentiation Protein 1