title: Human papillomavirus infection among women with cytological abnormalities in Switzerland investigated by an automated linear array genotyping test.
abstract: Limited data are available describing human papillomavirus (HPV) genotype distribution among females with cytological abnormalities in Switzerland. Cervical cell specimens obtained from 5,318 women were screened routinely by liquid-based Pap smear. All specimens with cellular abnormalities were analyzed subsequently for HPV DNA by the Linear Array HPV genotyping test. Cellular abnormalities were found in 202 (3.8%) specimens, of which 150 (74.3%) were positive for high-risk (HR) HPV. HR-HPV was detected in 20 (60.6%; 95% CI, 43.7-75.4%) of 33 specimens with atypical squamous cells of undetermined significance compared to 98 (72.1%; 95% CI, 64-78.9%) of 136 low-grade squamous intraepithelial lesions and 32 (97%; 95% CI, 83.4-99.9%) of 33 high-grade squamous intraepithelial lesions. The cumulative prevalence of HR-HPV other than HPV 16 and 18 was significantly higher than HPV 16 and/or 18 lesions with atypical squamous cells and low-grade lesions and was comparable in high-grade squamous intraepithelial lesions. The most common HR-HPV genotypes were HPV 16 (15.2%), HPV 31 (12.1%), HPV 58 (12.1%), HPV 51 (9.1%), and HPV 59 (9.1%) in women with atypical squamous cells, HPV 16 (25%), HPV 51 (16.9%), HPV 52 (11.8%), HPV 31 (9.6%), and HPV 56 (8.1%) in women with low-grade lesions (LSIL) and HPV 16 (57.6%), HPV 18 (18.2%), HPV 31 (15.2%), HPV 52 (12.1%), and HPV 58 (6.1%) in women with high-grade lesions (HSIL).
authors.affiliation: Medica, Medizinische Laboratorien Dr. F. Kaeppeli, Zurich, Switzerland. m.dobec@medica-labor.ch
authors.forename: Marinko
authors.initials: M
authors.lastname: Dobec
authors.forename: Fridolin
authors.initials: F
authors.lastname: Bannwart
authors.forename: Sandra
authors.initials: S
authors.lastname: Kilgus
authors.forename: Franz
authors.initials: F
authors.lastname: Kaeppeli
authors.forename: Pascal
authors.initials: P
authors.lastname: Cassinotti
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D065006:Papanicolaou Test; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000453:epidemiology*; D015995:Prevalence; D013557:Switzerland / Q000453:epidemiology; D002583:Uterine Cervical Neoplasms / Q000150:complications* / Q000821:virology*; D014626:Vaginal Smears; D055815:Young Adult
chemical_list: D004279:DNA, Viral