title: A laminar flow electroporation system for efficient DNA and siRNA delivery.
abstract: By introducing a hydrodynamic mechanism into a microfluidics-based electroporation system, we developed a novel laminar flow electroporation system with high performance. The laminar buffer flow implemented in the system separated the cell suspension flow from the electrodes, thereby excluding many unfavorable effects due to electrode reaction during electroporation, such as hydrolysis, bubble formation, pH change, and heating. Compared to conventional microfluidic electroporation systems, these improvements significantly enhanced transfection efficiency and cell viability. Furthermore, successful electrotransfection of plasmid DNA and, more importantly, synthetic siRNA, was demonstrated in several hard-to-transfect cell types using this system.
authors.affiliation: National Key Laboratory of Science and Technology on Micro/Nano Fabrication, Institute of Microelectronics, Peking University, Beijing, People's Republic of China.
authors.forename: Zewen
authors.initials: Z
authors.lastname: Wei
authors.forename: Deyao
authors.initials: D
authors.lastname: Zhao
authors.forename: Xueming
authors.initials: X
authors.lastname: Li
authors.forename: Mengxi
authors.initials: M
authors.lastname: Wu
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Huang
authors.initials: H
authors.lastname: Huang
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Wang
authors.forename: Quan
authors.initials: Q
authors.lastname: Du
authors.forename: Zicai
authors.initials: Z
authors.lastname: Liang
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Li
mesh_terms: D000818:Animals; D002460:Cell Line; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004566:Electrodes; D018274:Electroporation / Q000379:methods*; D006801:Humans; D044085:Microfluidics / Q000379:methods; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D034741:RNA, Small Interfering / Q000737:chemistry / Q000378:metabolism*; D051381:Rats
chemical_list: D034741:RNA, Small Interfering; D004247:DNA