title: Influence of acute cadmium exposure on the liver proteome of a teleost fish, ayu (Plecoglossus altivelis).
abstract: Cadmium (Cd) is a toxic heavy metal that causes the disruption of a variety of physiological processes. In this study, the effect of Cd on liver proteome of ayu, Plecoglossus altivelis, was investigated by two-dimensional gel electrophoresis (2-DE) and matrix assisted laser desorption ionization time-of-flight tandem mass spectrometry (MALDI-TOF-MS/MS). Twenty-three altered protein spots were successfully identified. They were involved in oxidative stress response, metal metabolism, methylation, and so on. The mRNA expression of 60S acidic ribosomal protein P0, heat shock protein 70, apolipoprotein A-I, betaine-homocysteine S-methyltransferase, parahox cluster neighbor, and transferrin was subsequently determined by real-time PCR. The mRNA expression of these genes was consistent with proteomic results. These findings enrich our knowledge on the influence of Cd toxicity to teleost fish, and may be worthy of further investigation to develop biomarkers.
authors.affiliation: Faculty of Life Science and Biotechnology, Ningbo University, Ningbo 315211, People's Republic of China.
authors.forename: Xin-Jiang
authors.initials: XJ
authors.lastname: Lu
authors.forename: Jiong
authors.initials: J
authors.lastname: Chen
authors.forename: Zuo-An
authors.initials: ZA
authors.lastname: Huang
authors.forename: Li
authors.initials: L
authors.lastname: Zhuang
authors.forename: Li-Zhen
authors.initials: LZ
authors.lastname: Peng
authors.forename: Yu-Hong
authors.initials: YH
authors.lastname: Shi
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002104:Cadmium / Q000633:toxicity*; D017931:DNA Primers; D015180:Electrophoresis, Gel, Two-Dimensional; D004781:Environmental Exposure; D029941:Fish Proteins / Q000378:metabolism*; D020869:Gene Expression Profiling; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D036441:Osmeriformes / Q000378:metabolism*; D020543:Proteome / Q000187:drug effects* / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D017931:DNA Primers; D029941:Fish Proteins; D020543:Proteome; D002104:Cadmium