title: Epigallocatechin-3-gallate induced modulation of cell deadhesion and migration on thermosensitive poly(N-isopropylacrylamide).
abstract: Epigallocatechin-3-gallate (EGCG), which is the main polyphenolic constituent of green tea, has emerged as a promising candidate for potential applications in selected anticancer therapeutics. Generally, tumor metastasis is known to be correlated with the alterations in cell adhesion and migration of normal cells. Nevertheless, the effect of EGCG on the biophysical responses of tumor cell adhering on extracellular matrix remains obscure. In this study, a thermosenstive poly(N-isopropylacrylamide) (PIPAAm) system was developed to elucidate the potential anti-tumor effect of EGCG on the deadhesion and migration of HepG2 cells. First, both XPS and ELISA validated the coating of laminin (LA) on PIPAAm. Second, a change of nanotopology of LA layer on PIPAAm across the lower solution critical temperature (LCST) was detected with AFM. HepG2 cells seeded on LA-coated PIPAAm surface was shown to go through deadhesion by lowering the temperature below the LCST. Interestingly, EGCG was shown to decelerate the thermally triggered deadhesion of HepG2 cell on LA coated PIPAAm. Moreover, the inhibition of cell deadhesion in EGCG treated cells was shown to be driven by actin remodeling. Interestingly, the modulation of cell deadhesion on LA coated PIPAAm by EGCG leads to the reduction of cell motility as shown by real-time cell migration assay. Overall, the use of PIPAAm system demonstrated the promise of EGCG as anticancer therapy through the suppression of cell deadhesion and migration.
authors.affiliation: Center of Biotechnology, School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore, Singapore.
authors.forename: Xi
authors.initials: X
authors.lastname: Li
authors.forename: Huixing
authors.initials: H
authors.lastname: Feng
authors.forename: Beiyi
authors.initials: B
authors.lastname: Chen
authors.forename: Soon Seng
authors.initials: SS
authors.lastname: Ng
authors.forename: Wei Ning
authors.initials: WN
authors.lastname: Chen
authors.forename: Vincent
authors.initials: V
authors.lastname: Chan
mesh_terms: D000178:Acrylamides / Q000737:chemistry*; D000180:Acrylic Resins; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D001672:Biocompatible Materials / Q000737:chemistry*; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects*; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy; D011108:Polymers / Q000737:chemistry*; D013662:Tea / Q000737:chemistry; D013696:Temperature
chemical_list: D000178:Acrylamides; D000180:Acrylic Resins; D016588:Anticarcinogenic Agents; D001672:Biocompatible Materials; D011108:Polymers; D013662:Tea; C052970:poly-N-isopropylacrylamide; D002392:Catechin; C045651:epigallocatechin gallate