title: Long-distance kissing loop interactions between a 3' proximal Y-shaped structure and apical loops of 5' hairpins enhance translation of Saguaro cactus virus.
abstract: Circularization of cellular mRNAs is a key event prior to translation initiation. We report that efficient translation of Saguaro cactus virus (SCV) requires a 3' translational enhancer (PTE) located partially in coding sequences. Unlike a similar PTE reported in the 3' UTR of Pea enation mosaic virus that does not engage in an RNA:RNA interaction (Wang Z. et al., J. Biol. Chem. 284, 14189-14202, 2009), the SCV PTE participates in long distance RNA:RNA interactions with hairpins located in the p26 ORF and in the 5' UTR of one subgenomic RNA. At least two additional RNA:RNA interactions are also present, one of which involves the p26 initiation codon. Similar PTE can be found in six additional carmoviruses that can putatively form long-distance interactions with 5' hairpins located in comparable positions.
authors.affiliation: Department of Cell Biology and Molecular Genetics, University of Maryland College Park, College Park, MD 20742, USA.
authors.forename: Maitreyi
authors.initials: M
authors.lastname: Chattopadhyay
authors.forename: Kerong
authors.initials: K
authors.lastname: Shi
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Yuan
authors.forename: Anne E
authors.initials: AE
authors.lastname: Simon
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics*; D020121:5' Untranslated Regions / Q000235:genetics*; D001483:Base Sequence; D029421:Cactaceae / Q000821:virology*; D019184:Carmovirus / Q000235:genetics*; D004742:Enhancer Elements, Genetic / Q000235:genetics*; D017930:Genes, Reporter; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D014176:Protein Biosynthesis / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics
chemical_list: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D012333:RNA, Messenger; D012367:RNA, Viral