title: Spontaneous uptake of exogenous DNA by goat spermatozoa and selection of donor bucks for sperm-mediated gene transfer.
abstract: Sperm-mediated gene transfer (SMGT) has been long heralded as a faster and cheaper alternative to more commonly used methods of producing transgenic animals. In this study, the capra semen ejaculates were pooled together and then incubated in vitro with DIG-labeled DNA. The binding and internalizing rates were observed by the in situ hybridization methods. We also compared the standard sperm parameters and the efficiencies of interaction with exogenous DNA of 60 individuals to select donor bucks for SMGT. It was showed that labeled exogenous DNA was detected in different localizations in spermatozoa but genuine DNA uptake, in contrast to mere binding, seems to be limited to the postacrosomal region. The removal of seminal plasma increased significantly (P < 0.01) the capability in picking up exogenous DNA. Use of frozen-thawed semen (without cryoprotectant agents) and Triton X-100 treatment also increased significantly (P < 0.01) the DNA-binding capacity, but reduced the sperm viability. The binding rates (the proportion of labeled-DNA positive spermatozoa to all the spermatozoa counted) of 60 buck individuals were in the range of 3.08-73.39%, and the internalizing rates (the proportion of DNaseI-treated labeled-DNA positive spermatozoa to all the spermatozoa counted) were 4.83-70.00%. About 8.34% (5/60) bucks showed high binding, but low internalizing ability. Chi-square test showed that there was significant difference among the breeds (x(2) = 26.515, P < 0.01). Eight individual bucks that demonstrated high DNA uptake were selected for SMGT. It was demonstrated that the goat spermatozoa was capable of spontaneous uptake of exogenous DNA. Seminal fluid inhibits DNA uptake and that membrane disruption increases DNA binding but greatly diminishes uptake.
authors.affiliation: College of Animal Science and Technology, Chongqing Key Laboratory of Forage & Herbivore, Southwest University, Beibei, Chongqing 400716, People's Republic of China. zyongju@163.com
authors.forename: Yongju
authors.initials: Y
authors.lastname: Zhao
authors.forename: Mingju
authors.initials: M
authors.lastname: Yu
authors.forename: Lingbin
authors.initials: L
authors.lastname: Wang
authors.forename: Yaofeng
authors.initials: Y
authors.lastname: Li
authors.forename: Jingsheng
authors.initials: J
authors.lastname: Fan
authors.forename: Qian
authors.initials: Q
authors.lastname: Yang
authors.forename: Yaping
authors.initials: Y
authors.lastname: Jin
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D030801:Animals, Genetically Modified; D001947:Breeding / Q000379:methods*; D004247:DNA / Q000378:metabolism*; D004705:Endocytosis / Q000235:genetics / Q000502:physiology; D018014:Gene Transfer Techniques / Q000662:veterinary*; D006041:Goats / Q000235:genetics / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D017403:In Situ Hybridization / Q000379:methods / Q000662:veterinary; D008297:Male; D017830:Octoxynol; D013094:Spermatozoa / Q000378:metabolism*
chemical_list: D049452:Green Fluorescent Proteins; D017830:Octoxynol; D004247:DNA