title: Separation of clavulanic acid from fermented broth of amino acids by an aqueous two-phase system and ion-exchange adsorption.
abstract: The clavulanic acid is a substance which inhibits the β-lactamases used with penicillins for therapeutic treatment. After the fermentation, by-products of low molecular weight such as amino acids lysine, histidine, proline and tyrosine are present in the fermented broth. To remove these impurities the techniques of extraction by an aqueous two-phase system of 17% polyethylene glycol molecular weight 600 and 15% potassium phosphate were used for a partial purification. A subsequent ion-exchange adsorption was used for the recuperation of the clavulanic acid of the top phase and purification getting a concentration factor of 2 and purification of 100% in relation to the amino acids lysine, histidine, proline and tyrosine.
authors.affiliation: Department of Chemical Engineering, Universidade Federal de São Carlos, Cx. Postal 676, CEP 13565-905, São Carlos, SP, Brazil.
authors.forename: Clovis Sacardo
authors.initials: CS
authors.lastname: da Silva
authors.forename: Maressa Fabiano
authors.initials: MF
authors.lastname: Cuel
authors.forename: Verônica Orlandin
authors.initials: VO
authors.lastname: Barreto
authors.forename: Wu Hong
authors.initials: WH
authors.lastname: Kwong
authors.forename: Carlos O
authors.initials: CO
authors.lastname: Hokka
authors.forename: M
authors.initials: M
authors.lastname: Barboza
mesh_terms: D000327:Adsorption; D000596:Amino Acids / Q000737:chemistry; D019818:Clavulanic Acid / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification*; D003470:Culture Media / Q000737:chemistry*; D007474:Ion Exchange; D013302:Streptomyces / Q000254:growth & development* / Q000378:metabolism
chemical_list: D000596:Amino Acids; D003470:Culture Media; D019818:Clavulanic Acid