title: Isolation and identification of novel microRNAs from Marsupenaeus japonicus.
abstract: MicroRNAs (miRNAs) are a class of small noncoding RNAs that function as regulators of gene expression. They play essential roles in various biological processes, such as development, differentiation and immune response. In this study, we identified 35 miRNAs from Marsupenaeus japonicus. Among them, fifteen miRNAs exhibited high homology to the known miRNAs from other arthropods, while the rest might represent novel miRNAs. We further showed a correlation of WSSV infection and the expression levels of 22 miRNAs. This is the first report to identify miRNAs from the shrimp. Our results extend the knowledge of the gene regulation of crustacean, providing clues for future researches of shrimp immunity against virus infection.
authors.affiliation: Key Laboratory of Marine Biogenetic Resources of SOA, Third Institute of Oceanography, State Oceanic Administration (SOA), Xiamen 361005, PR China.
authors.forename: Lingwei
authors.initials: L
authors.lastname: Ruan
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Bian
authors.forename: Yongchang
authors.initials: Y
authors.lastname: Ji
authors.forename: Mingyuan
authors.initials: M
authors.lastname: Li
authors.forename: Fang
authors.initials: F
authors.lastname: Li
authors.forename: Xiumin
authors.initials: X
authors.lastname: Yan
mesh_terms: D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D035683:MicroRNAs / Q000235:genetics / Q000302:isolation & purification*; D033561:Penaeidae / Q000235:genetics* / Q000821:virology; D046848:White spot syndrome virus 1 / Q000276:immunology
chemical_list: D035683:MicroRNAs