title: Polymorphisms in CYP1B1 modify the risk of idiopathic male infertility with abnormal semen quality.
abstract: BACKGROUND
  It is acknowledged that Cytochrome P450 1B1 (CYP1B1) plays a crucial role in metabolism and is involved in lots of diseases. We carried out this study to evaluate the association between CYP1B1 single nucleotide polymorphisms (SNPs) and male infertility in the Han-Chinese population with abnormal semen parameters.
  
  
  METHODS
  We genotyped five CYP1B1 polymorphisms by using TaqMan allelic discrimination assay and Genome Lab SNP-stream. A total of 591 idiopathic infertile men and 419 fertile controls were comprised in the research. Semen quality analysis was performed using computer assisted sperm analysis. According to semen parameters, we divided cases into 3 subgroups in the stratified analysis.
  
  
  RESULTS
  In our study, we only found genetic variant rs1056836 is correlated with idiopathic male infertility (P=0.012). Additionally, in strategy analysis, rs1056836 may decrease the risk of abnormal sperm motility (OR=0.11, 95% CI=0.01, 0.86). While other four variants showed no significant association with male infertility.
  
  
  CONCLUSIONS
  Our results suggested that polymorphism of CYP1B1 modified the risk of male infertility, and men harboring this polymorphism had lower risk of abnormal sperm parameters. These findings should be validated by more epidemiological and functional studies.
authors.affiliation: State Key Laboratory of Reproductive Medicine, Institute of Toxicology, School of Public Health, Nanjing Medical University, China.
authors.forename: Weiyue
authors.initials: W
authors.lastname: Hu
authors.forename: Hua
authors.initials: H
authors.lastname: Yang
authors.forename: Jie
authors.initials: J
authors.lastname: Sun
authors.forename: Qiuyi
authors.initials: Q
authors.lastname: Zhang
authors.forename: Jingjin
authors.initials: J
authors.lastname: Yang
authors.forename: Lu
authors.initials: L
authors.lastname: Lu
authors.forename: Jiacheng
authors.initials: J
authors.lastname: Zhang
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Qin
authors.forename: Yankai
authors.initials: Y
authors.lastname: Xia
authors.forename: Xinru
authors.initials: X
authors.lastname: Wang
mesh_terms: D000328:Adult; D001189:Aryl Hydrocarbon Hydroxylases / Q000235:genetics*; D016022:Case-Control Studies; D065633:Cytochrome P-450 CYP1B1; D006801:Humans; D007248:Infertility, Male / Q000201:enzymology / Q000235:genetics*; D008297:Male; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D012661:Semen
chemical_list: D001189:Aryl Hydrocarbon Hydroxylases; C585496:CYP1B1 protein, human; D065633:Cytochrome P-450 CYP1B1