title: tcf21+ epicardial cells adopt non-myocardial fates during zebrafish heart development and regeneration.
abstract: Recent lineage-tracing studies have produced conflicting results about whether the epicardium is a source of cardiac muscle cells during heart development. Here, we examined the developmental potential of epicardial tissue in zebrafish during both embryonic development and injury-induced heart regeneration. We found that upstream sequences of the transcription factor gene tcf21 activated robust, epicardium-specific expression throughout development and regeneration. Cre recombinase-based, genetic fate-mapping of larval or adult tcf21(+) cells revealed contributions to perivascular cells, but not cardiomyocytes, during each form of cardiogenesis. Our findings indicate that natural epicardial fates are limited to non-myocardial cell types in zebrafish.
authors.affiliation: Department of Cell Biology and Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC 27710, USA.
authors.forename: Kazu
authors.initials: K
authors.lastname: Kikuchi
authors.forename: Vikas
authors.initials: V
authors.lastname: Gupta
authors.forename: Jinhu
authors.initials: J
authors.lastname: Wang
authors.forename: Jennifer E
authors.initials: JE
authors.lastname: Holdway
authors.forename: Airon A
authors.initials: AA
authors.lastname: Wills
authors.forename: Yi
authors.initials: Y
authors.lastname: Fang
authors.forename: Kenneth D
authors.initials: KD
authors.lastname: Poss
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D002454:Cell Differentiation / Q000502:physiology; D019070:Cell Lineage / Q000502:physiology; D005455:Fluorescent Antibody Technique; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D006321:Heart / Q000196:embryology*; D009024:Morphogenesis / Q000235:genetics / Q000502:physiology*; D010496:Pericardium / Q000166:cytology* / Q000378:metabolism; D012038:Regeneration / Q000235:genetics / Q000502:physiology*; D014157:Transcription Factors / Q000378:metabolism*; D015027:Zebrafish / Q000196:embryology*; D029961:Zebrafish Proteins / Q000378:metabolism*
chemical_list: C556397:Tcf21 protein, zebrafish; D014157:Transcription Factors; D029961:Zebrafish Proteins