title: Miniaturized flow cytometry-based BCR-ABL immunoassay in detecting leptomeningeal disease.
abstract: Despite central nervous system (CNS) prophylactic programs limit leptomeningeal involvement in acute lymphoblastic leukemia (ALL), it can still occur in a restricted percentage of cases. The exact risk rate remains still unknown, and several factors are associated with an increased probability to develop CNS involvement. Among them, Philadelphia (Ph)-positive genotype seems to play a relevant role. Recently, a flow cytometric assay to detect BCR-ABL protein has been developed, but little is known about its possible employment in leptomeningeal disease. Here, we show the miniaturized application of the original assay for BCR-ABL oncoprotein detection in cerebrospinal fluid (CSF) samples.
authors.affiliation: CEINGE-Biotecnologie Avanzate, Naples, Italy.
authors.forename: F
authors.initials: F
authors.lastname: D'Alessio
authors.forename: P
authors.initials: P
authors.lastname: Mirabelli
authors.forename: E
authors.initials: E
authors.lastname: Mariotti
authors.forename: M
authors.initials: M
authors.lastname: Raia
authors.forename: R
authors.initials: R
authors.lastname: Di Noto
authors.forename: G
authors.initials: G
authors.lastname: Fortunato
authors.forename: A
authors.initials: A
authors.lastname: Camera
authors.forename: L
authors.initials: L
authors.lastname: Del Vecchio
mesh_terms: D003937:Diagnosis, Differential; D005434:Flow Cytometry / Q000379:methods*; D016044:Fusion Proteins, bcr-abl / Q000134:cerebrospinal fluid* / Q000235:genetics; D006801:Humans; D007118:Immunoassay; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000134:cerebrospinal fluid / Q000150:complications / Q000175:diagnosis* / Q000235:genetics; D008577:Meningeal Neoplasms / Q000134:cerebrospinal fluid / Q000150:complications / Q000175:diagnosis* / Q000235:genetics; D010677:Philadelphia Chromosome; D016133:Polymerase Chain Reaction; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000134:cerebrospinal fluid / Q000150:complications / Q000175:diagnosis* / Q000235:genetics; D012680:Sensitivity and Specificity
chemical_list: D016044:Fusion Proteins, bcr-abl