title: A new oral vaccine candidate based on the microencapsulation by spray-drying of inactivated Vibrio cholerae.
abstract: The aim of this work was to evaluate the microencapsulation by spray-drying of inactivated Vibrio cholerae, using methacrylic copolymers Eudragit® L30D-55 and FS30D. The microparticles obtained presented a particle size around 3.0 μm. The preparation temperature affected the morphology and the antigenicity of microparticles, but it did not affect the V. cholerae content. In vitro release studies showed that in acid medium less than 5% of bacteria was released, and in neutral medium, Eudragit® L30D-55 microparticles released 86% after 24 h, whereas FS30D released less than 30%. Rats inoculated with microparticles exhibited vibriocidal antibody titres. Microencapsulation by spray-drying of inactivated V. cholerae could be proposed as a method to obtain an oral vaccine which provides controlled release of the bacteria.
authors.affiliation: Finlay Institute, 27 Ave. #19805, La Lisa, Ciudad de la Habana, A.P. 16017, Cod.11600, Cuba.
authors.forename: Gemma
authors.initials: G
authors.lastname: Año
authors.forename: Amaia
authors.initials: A
authors.lastname: Esquisabel
authors.forename: Marta
authors.initials: M
authors.lastname: Pastor
authors.forename: Arturo
authors.initials: A
authors.lastname: Talavera
authors.forename: Bárbara
authors.initials: B
authors.lastname: Cedré
authors.forename: Sonsire
authors.initials: S
authors.lastname: Fernández
authors.forename: Sergio
authors.initials: S
authors.lastname: Sifontes
authors.forename: Yisabel
authors.initials: Y
authors.lastname: Aranguren
authors.forename: Gustavo
authors.initials: G
authors.lastname: Falero
authors.forename: Luis
authors.initials: L
authors.lastname: García
authors.forename: Rosa Lydia
authors.initials: RL
authors.lastname: Solís
authors.forename: José Luis
authors.initials: JL
authors.lastname: Pedraz
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002771:Cholera / Q000276:immunology* / Q000517:prevention & control; D022121:Cholera Vaccines / Q000008:administration & dosage* / Q000276:immunology*; D003890:Desiccation; D004339:Drug Compounding; D050296:Microbial Viability / Q000276:immunology; D008863:Microspheres; D011109:Polymethacrylic Acids; D051381:Rats; D015164:Vaccines, Inactivated / Q000276:immunology; D014734:Vibrio cholerae / Q000276:immunology*
chemical_list: D022121:Cholera Vaccines; D011109:Polymethacrylic Acids; D015164:Vaccines, Inactivated; C038300:methylmethacrylate-methacrylic acid copolymer