title: Differential effects of dietary canola and soybean oil intake on oxidative stress in stroke-prone spontaneously hypertensive rats.
abstract: BACKGROUND
  Canola oil shortens the life span of stroke-prone spontaneously hypertensive (SHRSP) rats compared with rats fed soybean oil when given as the sole dietary lipid source. One possible mechanism leading to the damage and deterioration of organs due to canola oil ingestion is oxidative stress. This study investigated the effect of canola oil intake on oxidative stress in this animal model.
  
  
  METHODS
  Male SHRSP rats, were fed a defatted control diet containing 10% wt/wt soybean oil or a defatted treatment diet containing 10% wt/wt canola oil, and given water containing 1% NaCl. Blood pressure was measured weekly. Blood was collected prior to beginning the diets and at the end of completion of the study for analysis of red blood cell (RBC) antioxidant enzymes, RBC and plasma malondialdehyde (MDA), plasma 8-isoprostane and plasma lipids.
  
  
  RESULTS
  Canola oil ingestion significantly decreased the life span of SHRSP rats compared with soybean oil, 85.8 ± 1.1 and 98.3 ± 3.4 days, respectively. Systolic blood pressure increased over time with a significant difference between the diets at the 6th week of feeding. Canola oil ingestion significantly reduced RBC superoxide dismutase, glutathione peroxidase and catalase activities, total cholesterol and low-density lipoprotein cholesterol compared with soybean oil. There were no significant differences in RBC MDA concentration between canola oil fed and soybean oil fed rats. In contrast, plasma MDA and 8-isoprostane concentration was significantly lower in the canola oil group compared to the soybean oil group.
  
  
  CONCLUSIONS
  In conclusion, canola oil ingestion shortens the life span of SHRSP rats and leads to changes in oxidative status, despite an improvement in the plasma lipids.
authors.affiliation: School of Medicine, Deakin University, Victoria, Australia.
authors.forename: Annateresa
authors.initials: A
authors.lastname: Papazzo
authors.forename: Xavier A
authors.initials: XA
authors.lastname: Conlan
authors.forename: Louise
authors.initials: L
authors.lastname: Lexis
authors.forename: Paul A
authors.initials: PA
authors.lastname: Lewandowski
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001794:Blood Pressure / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D004041:Dietary Fats / Q000008:administration & dosage / Q000494:pharmacology*; D004326:Drinking / Q000187:drug effects; D005229:Fatty Acids, Monounsaturated / Q000008:administration & dosage / Q000494:pharmacology*; D005247:Feeding Behavior / Q000187:drug effects*; D008055:Lipids / Q000097:blood; D008297:Male; D023421:Models, Animal; D018384:Oxidative Stress / Q000187:drug effects*; D000074262:Rapeseed Oil; D051381:Rats; D011918:Rats, Inbred SHR; D013024:Soybean Oil / Q000008:administration & dosage / Q000494:pharmacology*; D020521:Stroke / Q000097:blood / Q000473:pathology* / Q000503:physiopathology; D016019:Survival Analysis
chemical_list: D000975:Antioxidants; D004041:Dietary Fats; D005229:Fatty Acids, Monounsaturated; D008055:Lipids; D000074262:Rapeseed Oil; D013024:Soybean Oil