title: Nongenetic reprogramming of a fungal highly reducing polyketide synthase.
abstract: The biosynthesis of the fungal metabolite tenellin from Beauveria bassiana CBS110.25 was investigated in the presence of the epigenetic modifiers 5-azacytidine and suberoyl bis-hydroxamic acid and under conditions where individual genes from the tenellin biosynthetic gene cluster were silenced. Numerous new compounds were synthesized, indicating that the normal predominant biosynthesis of tenellin is just one outcome out of a diverse array of possible products. The structures of the products reveal key clues about the programming selectivities of the tenellin polyketide synthase.
authors.affiliation: School of Chemistry, University of Bristol, Cantock's Close, Bristol BS8 1TS, UK.
authors.forename: Ahmed A
authors.initials: AA
authors.lastname: Yakasai
authors.forename: Jack
authors.initials: J
authors.lastname: Davison
authors.forename: Zahida
authors.initials: Z
authors.lastname: Wasil
authors.forename: Laura M
authors.initials: LM
authors.lastname: Halo
authors.forename: Craig P
authors.initials: CP
authors.lastname: Butts
authors.forename: Colin M
authors.initials: CM
authors.lastname: Lazarus
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Bailey
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Simpson
authors.forename: Russell J
authors.initials: RJ
authors.lastname: Cox
mesh_terms: D052982:Beauveria / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D020868:Gene Silencing; D008958:Models, Molecular; D008968:Molecular Conformation; D010084:Oxidation-Reduction; D048630:Polyketide Synthases / Q000172:deficiency / Q000235:genetics* / Q000378:metabolism*; D011728:Pyridones / Q000737:chemistry / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D011728:Pyridones; D012333:RNA, Messenger; C015528:tenellin; D048630:Polyketide Synthases