title: Analysis of mammalian fatty acyl-coenzyme A species by mass spectrometry and tandem mass spectrometry.
abstract: Acyl-CoAs are intermediates of numerous metabolic processes in eukaryotic cells, including beta-oxidation within mitochondria and peroxisomes, and the biosynthesis/remodeling of lipids (e.g. mono-, di-, and triglycerides, phospholipids and sphingolipids). Investigations of lipid metabolism have been advanced by the ability to quantitate acyl-CoA intermediates via liquid chromatography coupled to electrospray ionization-tandem mass spectrometric detection (LC-ESI-MS/MS), which is presently one of the most sensitive and specific analytical methods for both lipids and acyl-CoAs. This review of acyl-CoA analysis by mass spectrometry focuses on mammalian samples and long-chain analytes (i.e. palmitoyl-CoA), particularly reports of streamlined methodology, improved recovery, or expansion of the number of acyl chain-lengths amenable to quantitation.
authors.affiliation: Newborn Screening Quality Assurance Program, NSQAP, USA. cph7@cdc.gov
authors.forename: Christopher A
authors.initials: CA
authors.lastname: Haynes
mesh_terms: D000214:Acyl Coenzyme A / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008322:Mammals / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000214:Acyl Coenzyme A