title: Regucalcin (RGN/SMP30) alters agonist- and thapsigargin-induced cytosolic [Ca2+] transients in cells by increasing SERCA Ca(2+)ATPase levels.
abstract: Regucalcin (RGN), also reported as senescence marker protein-30 (SMP30), plays a role in Ca(2+) homeostasis by modulating a number of Ca(2+)-dependent proteins. RGN also plays a cyto-protective role and its decrease is linked to age-related diseases and cell death. This study shows that RGN reduces agonist (histamine)-induced Ca(2+) transients in RGN(+) transfected COS-7 cells (RGN(+)) and also increases their Ca(2+) storage capacity. These observations are explained by RGN(+) cells having increased mRNA and protein expression levels of sarco/endoplasmic reticulum Ca(2+)-ATPase (SERCA). Therefore down-regulation of RGN expression may contribute to characteristics of age-dependent Ca(2+) homeostasis dis-regulation, by decreasing SERCA levels.
authors.affiliation: School of Biosciences, The University of Birmingham, Edgbaston, Birmingham, United Kingdom.
authors.forename: Pei
authors.initials: P
authors.lastname: Lai
authors.forename: Nga Chi
authors.initials: NC
authors.lastname: Yip
authors.forename: Francesco
authors.initials: F
authors.lastname: Michelangeli
mesh_terms: D000818:Animals; D019556:COS Cells; D002118:Calcium / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D002522:Chlorocebus aethiops; D004791:Enzyme Inhibitors / Q000378:metabolism; D006632:Histamine / Q000378:metabolism; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000235:genetics / Q000378:metabolism*; D019284:Thapsigargin / Q000378:metabolism*
chemical_list: D002135:Calcium-Binding Proteins; D004791:Enzyme Inhibitors; D047908:Intracellular Signaling Peptides and Proteins; C513323:RGN protein, human; D019284:Thapsigargin; D006632:Histamine; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases; D002118:Calcium