title: The E3 CR1-gamma gene in human adenoviruses associated with epidemic keratoconjunctivitis.
abstract: Human adenovirus species D type 37 (HAdV-D37) is an important etiologic agent of epidemic keratoconjunctivitis. Annotation of the whole genome revealed an open reading frame (ORF) in the E3 transcription unit predicted to encode a 31.6kDa protein. This ORF, also known as CR1-γ, is predicted to be an integral membrane protein containing N-terminal signal sequence, luminal, transmembrane, and cytoplasmic domains. HAdV-D19 (C), another viral pathogen causing epidemic keratoconjunctivitis, contains an ORF 100% identical to its HAdV-D37 homologue but only 66% identical to other HAdV-D homologues. Kinetics of RNA expression and confirmation of splicing to the adenovirus tripartite leader sequence suggest a role for the protein product of CR1-γ in the late stages of the viral replication cycle. Confocal microscopy is consistent with expression in the cytoplasm. Sequence analysis reveals a hypervariable luminal domain and a conserved cytoplasmic domain. The luminal domain is predicted to contain multiple N-glycosylation sites. The cytoplasmic domain contains a putative protein kinase C phosphorylation site and potential YXXϕ and dileucine (LL) motifs suggesting a potential role in modification of host proteins.
authors.affiliation: Howe Laboratory, Massachusetts Eye and Ear Infirmary, Department of Ophthalmology, Harvard Medical School, 243 Charles Street, Boston, MA 02114, USA.
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Robinson
authors.forename: Jaya
authors.initials: J
authors.lastname: Rajaiya
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Zhou
authors.forename: Gurdeep
authors.initials: G
authors.lastname: Singh
authors.forename: David W
authors.initials: DW
authors.lastname: Dyer
authors.forename: James
authors.initials: J
authors.lastname: Chodosh
mesh_terms: D017503:Adenovirus E3 Proteins / Q000235:genetics* / Q000378:metabolism; D000258:Adenovirus Infections, Human / Q000821:virology*; D000260:Adenoviruses, Human / Q000235:genetics* / Q000302:isolation & purification* / Q000502:physiology; D006801:Humans; D007637:Keratoconjunctivitis / Q000821:virology*; D011499:Protein Processing, Post-Translational; D012326:RNA Splicing; D012367:RNA, Viral / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014779:Virus Replication
chemical_list: D017503:Adenovirus E3 Proteins; D012367:RNA, Viral