title: Optical methods for sensing glucose.
abstract: This critical review covers the present state of the art in optical sensing of glucose. Following an introduction into the significance of (continuous) sensing of glucose and a brief look back, we discuss methods based on (a) monitoring the optical properties of intrinsically fluorescent or labeled enzymes, their co-enzymes and co-substrates; (b) the measurement of the products of enzymatic oxidation of glucose by glucose oxidase; (c) the use of synthetic boronic acids; (d) the use of Concanavalin A; and (e) the application of other glucose-binding proteins. We finally present an assessment in terms of the advantages and disadvantages of the various methods (237 references).
authors.affiliation: Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, D-93040 Regensburg, Germany.
authors.forename: Mark-Steven
authors.initials: MS
authors.lastname: Steiner
authors.forename: Axel
authors.initials: A
authors.lastname: Duerkop
authors.forename: Otto S
authors.initials: OS
authors.lastname: Wolfbeis
mesh_terms: D057075:Enzyme Assays; D005947:Glucose / Q000032:analysis*; D010084:Oxidation-Reduction; D013057:Spectrum Analysis / Q000379:methods*
chemical_list: D005947:Glucose