title: Rapid identification of Burkholderia pseudomallei and Burkholderia mallei by fluorescence in situ hybridization (FISH) from culture and paraffin-embedded tissue samples.
abstract: We evaluated newly developed probes for rapid identification of Burkholderia (B.) pseudomallei and B. mallei and differentiation from B. thailandensis by fluorescence in situ hybridization (FISH). FISH correctly identified 100% of the tested B. pseudomallei (11), B. mallei (11), and B. thailandensis (1) strains, excluded 100% of all tested negative controls (61), and allowed demonstration of B. pseudomallei infection in a paraffin-embedded spleen tissue sample of an experimentally infected mouse.
authors.affiliation: Department for Tropical Medicine at the Bernhard Nocht Institute, Bundeswehr Hospital Hamburg, Bernhard-Nocht-Strasse, Germany. hagen@bni-hamburg.de
authors.forename: Ralf M
authors.initials: RM
authors.lastname: Hagen
authors.forename: Hagen
authors.initials: H
authors.lastname: Frickmann
authors.forename: Mandy
authors.initials: M
authors.lastname: Elschner
authors.forename: Falk
authors.initials: F
authors.lastname: Melzer
authors.forename: Heinrich
authors.initials: H
authors.lastname: Neubauer
authors.forename: Yves P
authors.initials: YP
authors.lastname: Gauthier
authors.forename: Paul
authors.initials: P
authors.lastname: Racz
authors.forename: Sven
authors.initials: S
authors.lastname: Poppert
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques / Q000379:methods*; D042726:Burkholderia mallei / Q000235:genetics / Q000302:isolation & purification*; D016957:Burkholderia pseudomallei / Q000235:genetics / Q000302:isolation & purification*; D005456:Fluorescent Dyes; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D051379:Mice; D015345:Oligonucleotide Probes / Q000235:genetics; D010336:Pathology / Q000379:methods*; D012680:Sensitivity and Specificity; D013154:Spleen / Q000382:microbiology
chemical_list: D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes