title: Novel highly hydrophilic methacrylate-based monolithic column with mixed-mode of hydrophilic and strong cation-exchange interactions for pressurized capillary electrochromatography.
abstract: A novel highly hydrophilic polymethacrylate-based monolithic stationary phase based on the copolymerization of 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPS) and pentaerythritol triacrylate (PETA) was designed for pressurized capillary electrochromatography. A typical hydrophilic interaction chromatography mechanism could be observed when the content of acetonitrile (ACN) in the mobile phase exceeded 25%. Slight swelling or shrinking with mobile phases of different polarity was observed in permeability studies. Good retentions and efficient separations of polar analytes, such as neutral amides and phenols, were well achieved in hydrophilic interaction chromatography mode with only about 50% ACN content in the mobile phase. It was remarkably lower than the content of ACN (>90%) used on the hydrophilic polymethacrylate-based monoliths reported previously. Additionally, a mixed mode of hydrophilic interaction (HI) and strong cation-exchange (SCX) could be also obtained in the analysis of charged peptides, and high column efficiency up to 80,000 plates/m was achieved without peak tailing. The prepared hydrophilic stationary phase might provide a potential environmental friendly separation media for polar solutes as it consumes a low volume of organic solvents.
authors.affiliation: Institute of Food Safety and Environmental Monitoring, Fuzhou University, 350108, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Lin
authors.forename: Shaofeng
authors.initials: S
authors.lastname: Liu
authors.forename: Jian
authors.initials: J
authors.lastname: Lin
authors.forename: Xucong
authors.initials: X
authors.lastname: Lin
authors.forename: Zenghong
authors.initials: Z
authors.lastname: Xie
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D000178:Acrylamides / Q000737:chemistry; D000179:Acrylates / Q000737:chemistry; D000476:Alkanesulfonates / Q000737:chemistry; D053801:Capillary Electrochromatography / Q000295:instrumentation*; D002411:Cation Exchange Resins / Q000737:chemistry*; D002852:Chromatography, Ion Exchange / Q000295:instrumentation*; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D008689:Methacrylates / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D009994:Osmolar Concentration; D010455:Peptides / Q000737:chemistry; D011409:Propylene Glycols / Q000737:chemistry
chemical_list: D000097:Acetonitriles; D000178:Acrylamides; D000179:Acrylates; D000476:Alkanesulfonates; D002411:Cation Exchange Resins; D008689:Methacrylates; D010455:Peptides; D011409:Propylene Glycols; C056288:2-acrylamido-2-methylpropanesulfonate; C027992:pentaerythrityl triacrylate; C032159:acetonitrile