title: [Effect of different treating principles and formulas on expression of osteogenic factors in steroid-induced osteonecrosis of femoral head of chichen].
abstract: OBJECTIVE
  To investigate the effect of two treating principles and formulas, which are named 'invigorating spleen to remove phlem and promoting blood circulation to remove meridian obstruction' (Jianpi) and 'invigorate the kidney and promoting blood circulation to remove meridian obstruction' (Bushen), on the expression of osteogenic factors in steroid-induced osteonecrosis of the femoral head (SONFH), such as bone morphogenetic protein 2 (BMP2) and transforming growth factor beta1 (TGFbeta1) and Smads, as well as to explore and compare their mechanisms of prevention and treatment of SONFH.
  
  
  METHODS
  Animal model of SONFH was established by injection with methylprednisolone in chest muscle on chickens. 48 SONFH chickens were randomly assigned to model, Jianpi and Bushen group. Another 16 normal chickens served as control group. At the 8th and 16th week, the expression of BMP2, TGFbeta1, Smad4 and Smad7 of bilateral femoral heads were detected with immunohistochemistry.
  
  
  RESULTS
  The expression of BMP2, TGFbeta1 and Smad4 decreased, and Smad7 increased significantly in model group compared with control group. The expression of BMP2, TGFbeta1, Smad4 increased and Smad7 decreased significantly in Jianpi group at the 8th week compared with model group, and the same changes in Bushen group at the 16th week.
  
  
  CONCLUSIONS
  Both Jianpi and Bushen formulas exerted preventive and therapeutic activity on SONFH through regulating the expression of BMP2, TGFbeta1, Smad4 and Smad7 to promote bone repair. Notably Jianpi formula took effect earlier than Bushen formula
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. kongu0051@163.com
authors.forename: Xiangying
authors.initials: X
authors.lastname: Kong
authors.forename: Rong
authors.initials: R
authors.lastname: Wan
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Shifu
authors.initials: S
authors.lastname: Lin
authors.forename: Wei
authors.initials: W
authors.lastname: Guo
authors.forename: Na
authors.initials: N
authors.lastname: Lin
authors.forename: Huanyu
authors.initials: H
authors.lastname: Kong
authors.forename: Weiheng
authors.initials: W
authors.lastname: Chen
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism; D002645:Chickens; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D005271:Femur Head Necrosis / Q000139:chemically induced* / Q000378:metabolism* / Q000473:pathology; D008775:Methylprednisolone; D010012:Osteogenesis / Q000187:drug effects*; D011897:Random Allocation; D051901:Smad4 Protein / Q000378:metabolism; D051906:Smad7 Protein / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
chemical_list: D055396:Bone Morphogenetic Protein 2; D004365:Drugs, Chinese Herbal; D051901:Smad4 Protein; D051906:Smad7 Protein; D053773:Transforming Growth Factor beta1; D008775:Methylprednisolone