title: Evaluation of two automated capillary electrophoresis systems for human serum protein analysis.
abstract: OBJECTIVE
  We compared automated capillary electrophoresis (CE) systems Capillarys 2 from Sebia and V8 from Helena Biosciences Europe (Elitech) with the semi-automated Hydrasys-Hyrys agarose gel electrophoresis (AGE) from Sebia.
  
  
  METHODS
  We evaluated analytical performances and compared 129 fresh routine sera (group A) to 164 frozen pathologic samples with suspicion or antecedent of monoclonal component (MC) (group B). Immunofixation was then compared with immunotyping provided by both CE systems.
  
  
  RESULTS
  Analytical performances from both CE systems have proven suitable results for clinical use, with within-run and between-run coefficients of variation inferior or equal to 5.2% and 7.7%, respectively. A good correlation was found between AGE and CE with r-value ranging from 0.81 to 0.96 for both CE systems. We observed high MC detection sensitivities (>85%) of in electrophoretogram readings for both CE systems. MC identification using CE systems provided suitable concordance with immunofixation, although failing to detect some IgM proteins or free light chains.
  
  
  CONCLUSIONS
  Both Capillarys 2 and V8 are reliable automated CE systems for patient care.
authors.affiliation: Department of Biochemistry, Lapeyronie University Hospital, 371 avenue du doyen Gaston Giraud, 34295 Montpellier Cedex 5, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Chartier
authors.forename: Anne Marie
authors.initials: AM
authors.lastname: Boularan
authors.forename: Anne Marie
authors.initials: AM
authors.lastname: Dupuy
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Badiou
authors.forename: Anne Sophie
authors.initials: AS
authors.lastname: Bargnoux
authors.forename: Chantal
authors.initials: C
authors.lastname: Cognot
authors.forename: Jean François
authors.initials: JF
authors.lastname: Eliaou
authors.forename: Jean Paul
authors.initials: JP
authors.lastname: Cristol
mesh_terms: D057205:Automation, Laboratory; D001798:Blood Proteins / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005188:False Negative Reactions; D006801:Humans; D057230:Limit of Detection; D015203:Reproducibility of Results
chemical_list: D001798:Blood Proteins