title: High-intensity fluorescence imaging and sensitive electrochemical detection of cancer cells by using an extracellular supramolecular reticular DNA-quantum dot sheath.
abstract: Acting as a cage-type cellular probe, an extracellular supramolecular reticular DNA-quantum dot (QD) sheath has been developed for high-intensity fluorescence microscopy imaging and the sensitive electrochemical detection of Ramos cells. The extracellular supramolecular reticular DNA-QD sheath is constructed from layer-by-layer self-assembly of DNA-CdTe QD probes and DNA nanowire frameworks functionalized with a Ramos cell-binding aptamer. The DNA-QD sheath forms specifically and quickly on the surface of Ramos cells at physiological temperature, and the assembly of large numbers of DNA-CdTe QD probes on the surface of Ramos cells produces exceedingly high fluorescence intensity. Using the extracellular supramolecular reticular DNA-QD sheath as the cellular probe, Ramos cells can be clearly observed and easily distinguished from a mixture of multiple cancer cells by fluorescence microscopy imaging. Using the new cage-type cellular probe, a sensitive sandwich-type electrochemical strategy has also been developed to achieve accurate quantitative analysis of Ramos cells. Under the optimized conditions, Ramos cells can be detected quantitatively in a range from 10 to 1000 cells with a detection limit of 10 cells. This strategy presents a promising platform for highly sensitive and convenient evaluation of cancer cell levels.
authors.affiliation: State Key Laboratory Base of Eco-chemical Engineering, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, Shandong, P. R. of China.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhong
authors.forename: Qinglan
authors.initials: Q
authors.lastname: Zhang
authors.forename: Shusheng
authors.initials: S
authors.lastname: Zhang
mesh_terms: D045744:Cell Line, Tumor; D004247:DNA / Q000737:chemistry*; D055664:Electrochemical Techniques; D006801:Humans; D008856:Microscopy, Fluorescence; D009693:Nucleic Acid Hybridization / Q000379:methods; D045663:Quantum Dots; D012680:Sensitivity and Specificity
chemical_list: D004247:DNA