title: Decreased expression of Bmal2 in patients with Parkinson's disease.
abstract: Bmal1 is one of the central regulators of the clock machinery. Recently, we examined the expression profile of Bmal1 in total leukocytes for a 12h duration during the evening, overnight, and the morning, in subjects with Parkinson's disease (PD) and healthy controls. The results indicate that the expression of Bmal1 is significantly lower in PD patients versus control subjects. However, it is still unclear whether other key regulators of the clock machinery, especially Bmal2, the paralog of Bmal1, are also expressed differently in PD. To address this issue, the expression profiles of Bmal2, Clock, and Dec1 were examined in the same samples using real-time RT-PCR assay. The results show a difference in the expression pattern of Bmal2, but not Clock and Dec1. The expression of Bmal2 is significantly lower in PD at 21:00 h (p=0.005) and 00:00 h (p=0.025). These results together with our previous findings suggest that the molecular clock in total leukocytes is disturbed in PD patients.
authors.affiliation: Department of Neurology and Neurobiology, Xuanwu Hospital of Capital Medical University, Key Laboratory for Neurodegenerative Diseases of Ministry of Education, Beijing 100053, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Ding
authors.forename: Shu
authors.initials: S
authors.lastname: Liu
authors.forename: Yanpeng
authors.initials: Y
authors.lastname: Yuan
authors.forename: Qingling
authors.initials: Q
authors.lastname: Lin
authors.forename: Piu
authors.initials: P
authors.lastname: Chan
authors.forename: Yanning
authors.initials: Y
authors.lastname: Cai
mesh_terms: D056930:ARNTL Transcription Factors / Q000096:biosynthesis* / Q000097:blood; D056926:CLOCK Proteins / Q000096:biosynthesis* / Q000097:blood; D016022:Case-Control Studies; D002940:Circadian Rhythm / Q000235:genetics*; D006801:Humans; D010300:Parkinson Disease / Q000097:blood / Q000235:genetics*; D025521:Tumor Suppressor Proteins / Q000096:biosynthesis* / Q000097:blood
chemical_list: D056930:ARNTL Transcription Factors; C486615:BMAL2 protein, human; C403671:DELEC1 protein, human; D025521:Tumor Suppressor Proteins; D056926:CLOCK Proteins; C538943:CLOCK protein, human