title: Characterization of Beauveria bassiana MsW1 isolated from pine sawyers, Monochamus saltuarius.
abstract: Monochamus saltuarius is a vector for pine wilt disease that causes enormous damage to native pine trees in Korea. To develop a biological control method for this pine wilt disease vector, an entomopathogenic fungus was isolated from the cadaver of an adult M. saltuarius supporting fungal conidiation. This fungus was named MsW1 and identified as Beauveria bassiana by microscopic examination, PCR amplification using B. bassiana -specific primers and genetic sequencing of the ITS and EF1-α regions. Virulence tests against M. saltuarius were conducted with conidial suspensions (1 × 10(8) conidia/ml) of B. bassiana MsW1 in laboratory conditions. The median lethal times (LT(50)) of adults and larvae were 7.2 and 7 days, and 100% mortality was observed at 11 and 13 days after inoculation, respectively. This is the first characterization of B. bassiana from M. saltuarius.
authors.affiliation: Department of Agricultural Biology, College of Agriculture, Life & Environment Sciences, Chungbuk National University, Cheongju, Korea.
authors.forename: Tae Young
authors.initials: TY
authors.lastname: Shin
authors.forename: Jae Bang
authors.initials: JB
authors.lastname: Choi
authors.forename: Sung Min
authors.initials: SM
authors.lastname: Bae
authors.forename: Hyun Na
authors.initials: HN
authors.lastname: Koo
authors.forename: Jong Yul
authors.initials: JY
authors.lastname: Roh
authors.forename: Yeon Ho
authors.initials: YH
authors.lastname: Je
authors.forename: Byung Rae
authors.initials: BR
authors.lastname: Jin
authors.forename: Soo Dong
authors.initials: SD
authors.lastname: Woo
mesh_terms: D000818:Animals; D001483:Base Sequence; D052982:Beauveria / Q000145:classification / Q000302:isolation & purification / Q000472:pathogenicity*; D001517:Coleoptera / Q000382:microbiology*; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D014774:Virulence