title: Aberrant vegetative and reproductive development by overexpression and lethality by silencing of OsHAP3E in rice.
abstract: We generated transgenic rice plants overexpressing OsHAP3E which encodes a subunit of a CCAAT-motif binding HAP complex. The OsHAP3E-overexpressing plants showed various abnormal morphologies both in their vegetative and reproductive phases. The OsHAP3E-overexpressing plants were dwarf with erected leaves and similar to brassinosteroid mutants in the vegetative phase. In the reproductive phase, dense panicle was developed, and occasionally successive generation of lateral rachises and formation of double flowers were observed. These phenotypes indicate association of OsHAP3E with determination of floral meristem identity. On the other hand, repression of OsHAP3E by RNAi or by overexpressing chimeric repressor fusion constructs brought about lethality to transformed cells, and almost no transformant was obtained. This suggests that the OsHAP3E function is essential for rice cells. Altogether, our loss-of-function and gain-of-function analyses suggest that OsHAP3E plays important pleiotropic roles in vegetative and reproductive development or basic cellular processes in rice.
authors.affiliation: Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai 981-8555, Japan. yukito@bios.tohoku.ac.jp
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Ito
authors.forename: Thiruvengadam
authors.initials: T
authors.lastname: Thirumurugan
authors.forename: Akiko
authors.initials: A
authors.lastname: Serizawa
authors.forename: Keiichiro
authors.initials: K
authors.lastname: Hiratsu
authors.forename: Masaru
authors.initials: M
authors.lastname: Ohme-Takagi
authors.forename: Nori
authors.initials: N
authors.lastname: Kurata
mesh_terms: D035264:Flowers / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D012275:Oryza / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D010641:Phenotype; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics; D034622:RNA Interference; D018749:RNA, Plant / Q000235:genetics; D014170:Transformation, Genetic
chemical_list: D010940:Plant Proteins; D018749:RNA, Plant