title: Spoligotyping of clinical Mycobacterium tuberculosis isolates from the state of Minas Gerais, Brazil.
abstract: We performed spoligotyping on 114 strains of the Mycobacterium tuberculosis (Mtb) complex that had been isolated from patients in Minas Gerais Health Units during 2004. A total of 82/114 (72%) clinical isolates were clustered and 32/114 (28%) were unique. Seven shared types containing nine strains were newly created. A total of nine patterns corresponded to unreported orphan strains, as evaluated against all of the strains recorded in the SITVIT2 proprietary database in the Institut Pasteur de la Guadeloupe. The major clades were composed of isolates that belong to the following genotypes: Latin-America and Mediterranean (63/114, 55.3%) (the ill-defined T superfamily) (12/114, 10.5%), Haarlem (8/114, 7%), X clade (6/114, 5.3%), S clade (3/114, 2.6%) and the East-African Indian and Manu types, each with 1/114 (0.9%) isolates. A considerable number of strains (n = 20, 17.5%) showed patterns that did not fall within any of the previously described major clades. We conclude the bulk of tuberculosis (TB) (92/114, 80.7%) in our location is recent evolutionary strains that belong to the principal genetic groups 2/3. Further studies on epidemiology of TB are required to understand Mtb biodiversity and TB transmission in this region.
authors.affiliation: Departamento de Clínica Médica/Pneumologia, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil. silvanaspindola@gmail.com
authors.forename: Silvana Spíndola de
authors.initials: SS
authors.lastname: Miranda
authors.forename: Wânia da Silva
authors.initials: Wda S
authors.lastname: Carvalho
authors.forename: Philip Noel
authors.initials: PN
authors.lastname: Suffys
authors.forename: Afrânio Lineu
authors.initials: AL
authors.lastname: Kritski
authors.forename: Martha
authors.initials: M
authors.lastname: Oliveira
authors.forename: Noemi
authors.initials: N
authors.lastname: Zarate
authors.forename: Thierry
authors.initials: T
authors.lastname: Zozio
authors.forename: Nalin
authors.initials: N
authors.lastname: Rastogi
authors.forename: Brigitte
authors.initials: B
authors.lastname: Gicquel
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods*; D016000:Cluster Analysis; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification