title: Polyethylenimine bioconjugates for imaging and DNA delivery in vivo.
abstract: Polyamine polymers are among the commonest polymers used in biomedicine. Among polyamine -polymers, polyethylenimine (PEI) may be used as an efficient delivery vehicle for nucleic acids (DNA, RNA, etc.) or employed as a versatile imaging probe in vivo. In this chapter, the preparation of various PEI bioconjugates will be fully explained and discussed.
authors.affiliation: Gene Expression-Microarrays Laboratory, IRCCS-Bambino Gesù Children's Hospital, Rome, Italy. andrea.masotti@opbg.net
authors.forename: Andrea
authors.initials: A
authors.lastname: Masotti
authors.forename: Francesco
authors.initials: F
authors.lastname: Pampaloni
mesh_terms: D000818:Animals; D004247:DNA / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D004337:Drug Carriers / Q000737:chemistry* / Q000633:toxicity; D004587:Electrophoresis, Agar Gel; D005612:Freeze Drying; D057927:Hydrophobic and Hydrophilic Interactions; D007208:Indocyanine Green / Q000031:analogs & derivatives / Q000737:chemistry; D007474:Ion Exchange; D008027:Light; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008856:Microscopy, Fluorescence; D057054:Molecular Imaging / Q000379:methods*; D041681:NIH 3T3 Cells; D011094:Polyethyleneimine / Q000737:chemistry* / Q000633:toxicity; D011199:Potentiometry; D012542:Scattering, Radiation; D012995:Solubility; D014162:Transfection / Q000379:methods*; D014462:Ultrafiltration
chemical_list: D004337:Drug Carriers; C541053:IR 820; D011094:Polyethyleneimine; D004247:DNA; D007208:Indocyanine Green