title: Identification and cloning of a selenophosphate synthetase (SPS) from tiger shrimp, Penaeus monodon, and its transcription in relation to molt stages and following pathogen infection.
abstract: Complementary (c)DNA encoding selenophosphate synthetase (SPS) messenger (m)RNA of the tiger shrimp Penaeus monodon, designated PmSPS, was obtained from the hepatopancreas by a reverse-transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). The 1582-bp cDNA contained an open reading frame (ORF) of 1248 bp, a 103-bp 5'-untranslated region (UTR), and a 231-bp 3'-UTR, which contained a conserved selenocysteine insertion sequence (SECIS) element, a conventional polyadenylation signal, and a poly A tail. The molecular mass of the deduced amino acid (aa) sequence (416 aa) was 45.5 kDa with an estimated pI of 4.85. It contained a putative selenocysteine residue which was encoded by the unusual stop codon, (275)TGA(277), which formed at the active site with residues Sec(58) and Lys(61). A comparison of amino acid sequences showed that PmSPS was more closely related to invertebrate SPS1, such as those of Heliothis virescens and Drosophila melanogaster a and b. PmSPS cDNA was synthesized in all tested tissues, especially in the hepatopancreas. PmSPS in the hepatopancreas of shrimp significantly increased after an injection with either Photobacterium damsela or white spot syndrome virus (WSSV) in order to protect cells against damage from oxidation, and enhance the recycling of selenocysteine or selenium metabolism, indicating that PmSPS is involved in the disease-resistance response. The PmSPS expression by hemocytes significantly increased in stage C, and then gradually decreased until stage A, suggesting that the cloned PmSPS in hemocytes might play a role in viability by renewing hemocytes and antioxidative stress response for new exoskeleton synthesis during the molt cycle of shrimp.
authors.affiliation: Institute of Biomedical Nutrition, Hungkuang University, Taichung 43302, Taiwan, ROC.
authors.forename: Maw-Sheng
authors.initials: MS
authors.lastname: Yeh
authors.forename: Chang-Jen
authors.initials: CJ
authors.lastname: Huang
authors.forename: Chih-Hung
authors.initials: CH
authors.lastname: Guo
authors.forename: Kuan-Fu
authors.initials: KF
authors.lastname: Liu
authors.forename: Inn-Ho
authors.initials: IH
authors.lastname: Tsai
authors.forename: Winton
authors.initials: W
authors.lastname: Cheng
mesh_terms: D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004331:Drosophila melanogaster / Q000235:genetics; D019143:Evolution, Molecular; D006434:Hemocytes / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology / Q000821:virology; D007109:Immunity / Q000235:genetics; D007239:Infections / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D008969:Molecular Sequence Data; D018978:Molting / Q000235:genetics; D018384:Oxidative Stress / Q000235:genetics; D033561:Penaeidae; D010770:Phosphotransferases / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D010776:Photobacterium / Q000276:immunology* / Q000472:pathogenicity; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D015533:Transcriptional Activation; D046848:White spot syndrome virus 1 / Q000276:immunology* / Q000472:pathogenicity
chemical_list: D010770:Phosphotransferases; C062762:selenophosphate synthetase