title: MAP3738c and MptD are specific tags of Mycobacterium avium subsp. paratuberculosis infection in type I diabetes mellitus.
abstract: Mycobacterium avium subsp. paratuberculosis (Map) is the causative agent of Johne's disease, a chronic inflammation of ruminants' intestine. Recent studies have linked Map to type I Diabetes mellitus (T1DM). We searched the presence of antibodies against two specific proteins of Map (MptD and MAP3738c) in sera of patients affected by T1DM and type II Diabetes mellitus (T2DM). MptD protein (MAP3733c) has been recognized as a Map virulent factor whereas MAP3738c has not yet been studied. Both proteins are encoded by genes belonging to a Map specific pathogenicity island. Forty three T1DM patients' sera, 56 T2DM patients' sera and 48 healthy subjects' sera were screened by ELISA to evaluate the immunoresponse against MptD or MAP3738c recombinant proteins. Results showed a positive response to both proteins in T1DM patients whereas no difference with controls was found for T2DM patients. Results suggest a potential relation between T1DM and the bacterial infection.
authors.affiliation: Department of Biomedical Sciences, Division of Experimental and Clinical Microbiology, University of Sassari, Sassari, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Cossu
authors.forename: Valentina
authors.initials: V
authors.lastname: Rosu
authors.forename: Daniela
authors.initials: D
authors.lastname: Paccagnini
authors.forename: Davide
authors.initials: D
authors.lastname: Cossu
authors.forename: Adolfo
authors.initials: A
authors.lastname: Pacifico
authors.forename: Leonardo Antonio
authors.initials: LA
authors.lastname: Sechi
mesh_terms: D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology; D001483:Base Sequence; D016022:Case-Control Studies; D004269:DNA, Bacterial / Q000235:genetics; D048909:Diabetes Complications / Q000276:immunology* / Q000382:microbiology*; D003922:Diabetes Mellitus, Type 1 / Q000150:complications* / Q000276:immunology / Q000382:microbiology*; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000276:immunology / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005798:Genes, Bacterial; D006801:Humans; D008297:Male; D008875:Middle Aged; D016927:Mycobacterium avium subsp. paratuberculosis / Q000235:genetics / Q000276:immunology*; D010283:Paratuberculosis / Q000150:complications* / Q000276:immunology* / Q000382:microbiology; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D055815:Young Adult
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D011994:Recombinant Proteins