title: Simultaneous analysis of chlorpyrifos and cypermethrin in cord blood plasma by online solid-phase extraction coupled with liquid chromatography-heated electrospray ionization tandem mass spectrometry.
abstract: Chlorpyrifos and cypermethrin are the most used insecticides in Taiwan. Exposure to both pesticides has been associated with reproductive and developmental health effects in humans and animals. This study describes an online solid-phase extraction coupled with liquid chromatography-heated electrospray ionization tandem mass spectrometry (online SPE-LC/HESI/MS/MS) method to analyze chlorpyrifos and cypermethrin in cord blood of pregnant women. Calibration curves showed good linearity (r² > 0.998) for both pesticides within the range of 0.1-100 ppb. Limits of detection (LODs) were 0.01 and 0.05 ppb and recoveries in cord blood were 97.2 ± 4.8% and 93.5 ± 9.5% for chlorpyrifos and cypermethrin respectively. After analysis of 396 samples, the mean concentrations of chlorpyrifos and cypermethrin were 0.38 and 1.08 ppb respectively. These results demonstrate that LC/HESI/MS/MS is effective for the simultaneous analysis of chlorpyrifos and cypermethrin in cord blood with excellent sensitivity and specificity and may also be effective for high throughput assay in future epidemiology studies.
authors.affiliation: Institute of Occupational Medicine and Industrial Hygiene, College of Public Health, National Taiwan University, Taipei, Taiwan.
authors.forename: Ho-Tang
authors.initials: HT
authors.lastname: Liao
authors.forename: Chia-Jung
authors.initials: CJ
authors.lastname: Hsieh
authors.forename: Su-Yin
authors.initials: SY
authors.lastname: Chiang
authors.forename: Ming-Huan
authors.initials: MH
authors.lastname: Lin
authors.forename: Pau-Chung
authors.initials: PC
authors.lastname: Chen
authors.forename: Kuen-Yuh
authors.initials: KY
authors.lastname: Wu
mesh_terms: D004390:Chlorpyrifos / Q000097:blood*; D002853:Chromatography, Liquid / Q000379:methods*; D005260:Female; D005312:Fetal Blood / Q000737:chemistry*; D006801:Humans; D010575:Pesticides / Q000097:blood / Q000302:isolation & purification; D011247:Pregnancy; D011722:Pyrethrins / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D010575:Pesticides; D011722:Pyrethrins; C017160:cypermethrin; D004390:Chlorpyrifos