title: Advanced multiplex PCR assay for differentiation of Brucella species.
abstract: Two new primer sets of a 766- and a 344-bp fragment were introduced into the conventional Bruce-ladder PCR assay. This novel multiplex PCR assay rapidly and concisely discriminates Brucella canis and Brucella microti from Brucella suis strains and also may differentiate all of the 10 Brucella species.
authors.affiliation: OIE Reference Laboratory for Brucellosis, Department of Bacteriology, National Veterinary Research and Quarantine Service (NVRQS), Anyang, Gyeonggi-do, South Korea.
authors.forename: Sung-Il
authors.initials: SI
authors.lastname: Kang
authors.forename: Moon
authors.initials: M
authors.lastname: Her
authors.forename: Jong Wan
authors.initials: JW
authors.lastname: Kim
authors.forename: Ji-Yeon
authors.initials: JY
authors.lastname: Kim
authors.forename: Kyung Yuk
authors.initials: KY
authors.lastname: Ko
authors.forename: Yun-Mi
authors.initials: YM
authors.lastname: Ha
authors.forename: Suk Chan
authors.initials: SC
authors.lastname: Jung
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D001483:Base Sequence; D002002:Brucella / Q000145:classification* / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D008969:Molecular Sequence Data; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers