title: Towards hemerythrin-based blood substitutes: comparative performance to hemoglobin on human leukocytes and umbilical vein endothelial cells.
abstract: Hemerythrin is a dioxygen-carrying protein whose oxidative/nitrosative stress-related reactivity is lower than that of hemoglobin, which may warrant investigation of hemerythrin as raw material for artificial oxygen carriers ('blood substitutes'). We report here the first biological tests for hemerythrin and its chemical derivatives, comparing their performance with that of a representative competitor, glutaraldehyde-polymerized bovine hemoglobin. Hemerythrin (native or derivatized) exhibits a proliferative effect on human umbilical vein endothelial cell (HUVEC) cultures, as opposed to a slight inhibitory effect of hemoglobin. A similar positive effect is displayed on human lymphocytes by glutaraldehyde-polymerized hemerythrin, but not by native or polyethylene glycol-derivatized hemerythrin.
authors.affiliation: Ion Chiricuta Cancer Institute-Comprehensive Cancer Center, 34-36 Republicii St, Cluj-Napoca 400015, Romania.
authors.forename: Eva
authors.initials: E
authors.lastname: Fischer-Fodor
authors.forename: Augustin
authors.initials: A
authors.lastname: Mot
authors.forename: Florina
authors.initials: F
authors.lastname: Deac
authors.forename: Mariann
authors.initials: M
authors.lastname: Arkosi
authors.forename: Radu
authors.initials: R
authors.lastname: Silaghi-Dumitrescu
mesh_terms: D000328:Adult; D000818:Animals; D001802:Blood Substitutes / Q000737:chemistry / Q000494:pharmacology*; D002417:Cattle; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003432:Cross-Linking Reagents / Q000737:chemistry; D042783:Endothelial Cells / Q000187:drug effects / Q000502:physiology*; D005976:Glutaral / Q000737:chemistry; D006422:Hemerythrin / Q000737:chemistry / Q000494:pharmacology*; D006454:Hemoglobins / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D007962:Leukocytes / Q000187:drug effects / Q000502:physiology*; D008297:Male; D014471:Umbilical Veins / Q000166:cytology*
chemical_list: D001802:Blood Substitutes; D003432:Cross-Linking Reagents; D006422:Hemerythrin; D006454:Hemoglobins; D005976:Glutaral