title: Anti-ATP synthase autoantibodies from patients with Alzheimer's disease reduce extracellular HDL level.
abstract: Aside from being an integral protein involved in the synthesis and hydrolysis of ATP, Ecto-F1-ATPase plays a role in cholesterol homeostasis. We demonstrated the presence of autoantibodies to ecto-F1-ATPase (ASabs) in sera and cerebrospinal fluids from patients with Alzheimer's disease (AD). Herein we show that ASabs, unlike irrelevant antibodies, can increase cellular uptake of HDL, a risk factor for the development of AD, via a mechanism involving the prototypical function of ecto-F1-ATPase: the generation of ADP due to the hydrolysis of ATP. Piceatannol, a specific inhibitor ecto-F1-ATPase, completely hindered these effects. We hypothesize that ASabs could exert a pathogenetic role in AD.
authors.affiliation: Department of Cell Biology and Neurosciences, Istituto Superiore di Sanità, Rome, Italy.
authors.forename: Davide
authors.initials: D
authors.lastname: Vacirca
authors.forename: Cristiana
authors.initials: C
authors.lastname: Barbati
authors.forename: Beatrice
authors.initials: B
authors.lastname: Scazzocchio
authors.forename: Roberta
authors.initials: R
authors.lastname: Masella
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Rosano
authors.forename: Walter
authors.initials: W
authors.lastname: Malorni
authors.forename: Elena
authors.initials: E
authors.lastname: Ortona
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000201:enzymology* / Q000276:immunology; D000918:Antibody Specificity; D017209:Apoptosis / Q000187:drug effects; D001323:Autoantibodies / Q000276:immunology / Q000494:pharmacology*; D002460:Cell Line; D008076:Cholesterol, HDL / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005110:Extracellular Space / Q000378:metabolism; D005260:Female; D005456:Fluorescent Dyes; D006801:Humans; D008297:Male; D008875:Middle Aged; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors* / Q000134:cerebrospinal fluid / Q000276:immunology*; D013267:Stilbenes / Q000494:pharmacology
chemical_list: D001323:Autoantibodies; D008076:Cholesterol, HDL; D004791:Enzyme Inhibitors; D005456:Fluorescent Dyes; D013267:Stilbenes; D000244:Adenosine Diphosphate; C041525:3,3',4,5'-tetrahydroxystilbene; D000255:Adenosine Triphosphate; D006180:Proton-Translocating ATPases