title: Small heat shock protein LimHSP16.45 protects pollen mother cells and tapetal cells against extreme temperatures during late zygotene to pachytene stages of meiotic prophase I in David Lily.
abstract: Plant meiotic prophase I is a complicated process involving the late zygotene and pachytene stages, both crucial for completing synapsis and recombination. Using David Lily (Lilium davidii var. Willmottiae) as our research material, we performed suppression subtractive hybridization to construct EST library of anthers at various stages of development by the pollen mother cells. From this library, we identified small heat shock protein LimHSP16.45 was highly expressed during the late zygotene to pachytene stages. Our results also showed that LimHSP16.45 was almost specifically expressed in the anther compared with the root, stem, or leaf, and in situ expression of LimHSP16.45 mRNAs showed strong signals in the pollen mother cells and tapetal cells. LimHSP16.45 could be induced by heat and cold in lily anthers, and its ectopic expression enhanced the viability of E. coli cells under both high and low temperatures. In vitro, it acted as molecular chaperone and could help luciferase refolding after heat shock stress. All of these data suggest that LimHSP16.45, working as molecular chaperone, possibly protects pollen mother cells and tapetal cells against extreme temperatures during late zygotene to pachytene stages of meiotic prophase I in David Lily.
authors.affiliation: Institute of Cell Biology, School of Life Sciences, Lanzhou University, Lanzhou, 730000, People's Republic of China.
authors.forename: Changjun
authors.initials: C
authors.lastname: Mu
authors.forename: Shaobo
authors.initials: S
authors.lastname: Wang
authors.forename: Shijia
authors.initials: S
authors.lastname: Zhang
authors.forename: Jiajia
authors.initials: J
authors.lastname: Pan
authors.forename: Ni
authors.initials: N
authors.lastname: Chen
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Li
authors.forename: Zhaoyan
authors.initials: Z
authors.lastname: Wang
authors.forename: Heng
authors.initials: H
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D003080:Cold Temperature; D019295:Computational Biology; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D050888:Heat-Shock Proteins, Small / Q000235:genetics / Q000378:metabolism*; D006358:Hot Temperature; D017403:In Situ Hybridization; D027762:Lilium / Q000235:genetics* / Q000378:metabolism; D018832:Molecular Chaperones / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D049471:Pachytene Stage; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D011058:Pollen / Q000235:genetics / Q000254:growth & development*; D018749:RNA, Plant / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D050888:Heat-Shock Proteins, Small; D018832:Molecular Chaperones; D010940:Plant Proteins; D018749:RNA, Plant