title: GATA3 is redundant for maintenance and self-renewal of hematopoietic stem cells.
abstract: GATA3 has been identified as a master regulator of T helper cells, as well as being important for early thymic progenitors and T-cell commitment. However, Gata3 expression initiates already at the hematopoietic stem cell (HSC) level, implicating a potential role also in the regulation of HSCs. Herein we used a conditional Gata3 knockout strategy in which Gata3 expression was completely deleted from the earliest stage of embryonic hematopoietic development after emergence of HSCs from hemogenic endothelium. Through a detailed analysis of HSCs at the phenotypic and functional level, we demonstrate that steady-state levels of HSCs are normal in Gata3(fl/fl)Vav-Cre(tg/+) mice. Moreover, through long-term primary and secondary transplantation experiments, we also unequivocally demonstrate that Gata3 has a redundant role in post-transplantation HSC self-renewal.
authors.affiliation: Haematopoietic Stem Cell Laboratory, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford, Oxford, United Kingdom.
authors.forename: Natalija
authors.initials: N
authors.lastname: Buza-Vidas
authors.forename: Sara
authors.initials: S
authors.lastname: Duarte
authors.forename: Sidinh
authors.initials: S
authors.lastname: Luc
authors.forename: Tiphaine
authors.initials: T
authors.lastname: Bouriez-Jones
authors.forename: Petter S
authors.initials: PS
authors.lastname: Woll
authors.forename: Sten Eirik Waelgaard
authors.initials: SE
authors.lastname: Jacobsen
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D002478:Cells, Cultured; D050990:GATA3 Transcription Factor / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D006410:Hematopoiesis / Q000502:physiology; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology
chemical_list: D050990:GATA3 Transcription Factor; C494748:Gata3 protein, mouse