title: A lab-on-a-chip for rapid blood separation and quantification of hematocrit and serum analytes.
abstract: In this work, a new lab-on-a-chip for rapid analysis of low volume blood samples was designed, fabricated and demonstrated for integration of serum separation, hematocrit evaluation, and protein quantitation. Blood separation was achieved using microchannel flow-based separation. A novel method for evaluating hematocrit from microfluidic flow-separated blood samples was developed using gray scale analysis of a point-and-shoot digital photograph of separated blood in a micochannel. Protein quantitation was subsequently performed in a high surface area-to-volume ratio microfluidic chemiluminescent immunoassay using cell depleted serum produced by microfluidic flow-based separation of whole blood samples. All three steps were achieved in a single microchannel with separation of blood samples and hematocrit evaluation in less than 1 min, and protein quantitation in 5 min.
authors.affiliation: Microsystems and BioMEMS Laboratory, Department of Electrical and Computer Engineering, University of Cincinnati, Cincinnati, OH 45221, USA. browneaw@mail.uc.edu
authors.forename: Andrew W
authors.initials: AW
authors.lastname: Browne
authors.forename: Lakshminarayanan
authors.initials: L
authors.lastname: Ramasamy
authors.forename: Timothy P
authors.initials: TP
authors.lastname: Cripe
authors.forename: Chong H
authors.initials: CH
authors.lastname: Ahn
mesh_terms: D001798:Blood Proteins / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D004912:Erythrocytes / Q000166:cytology*; D006400:Hematocrit / Q000379:methods*; D056656:Lab-On-A-Chip Devices; D008163:Luminescent Measurements / Q000379:methods*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods
chemical_list: D001798:Blood Proteins