title: Determination of genetic relationships among elite thermosensitive genic male sterile lines (TGMS) of rice (Oryza sativa L.) employing morphological and simple sequence repeat (SSR) markers.
abstract: A set of morphological traits and SSR markers were used to determine the genetic relationship among 12 elite thermosensitive genic male sterile (TGMS) lines developed at three different research institutions of India. Agro-morphological data recorded on 20 morphological traits revealed a wide base of genetic variation and a set of four morphological traits could distinguish most of the TGMS lines. Analysis with 30 SSR markers (20 EST-SSRs and 10 genomic SSRs) revealed 27 markers to be polymorphic, amplifying a total of 83 alleles. Each SSR marker amplified 2-6 alleles with an average of 2.76 alleles per marker and a PIC value varying from 0.54 to 0.96. Cluster analysis based on SSR and morphological data clearly differentiated the lines according to their source of origin. Correlation analysis between morphological and molecular data revealed a very poor association (r = 0.06), which could be attributed to selection pressure, genetic drift, sampling error and unknown relationship among related lines. The SSR markers discriminated the genotypes distinctly and quantified the genetic diversity precisely among the TGMS lines. Data on the yield per plant indicated that the genotypes grouping under a similar cluster showed same heterotic behaviour as compared to the genotypes from different clusters when crossed to similar pollinators.
authors.affiliation: Allahabad Agricultural Institute-Deemed University, Allahabad 211 007, India. vikasaaidu@gmail.com
authors.forename: Vikas Kumar
authors.initials: VK
authors.lastname: Singh
authors.forename: Priti
authors.initials: P
authors.lastname: Upadhyay
authors.forename: Pallavi
authors.initials: P
authors.lastname: Sinha
authors.forename: Ashish Kumar
authors.initials: AK
authors.lastname: Mall
authors.forename: Sanjay Kumar
authors.initials: SK
authors.lastname: Jaiswal
authors.forename: Atul
authors.initials: A
authors.lastname: Singh
authors.forename: Ranjith Kumar
authors.initials: RK
authors.lastname: Ellur
authors.forename: Sunil
authors.initials: S
authors.lastname: Biradar
authors.forename: R M
authors.initials: RM
authors.lastname: Sundaram
authors.forename: Sukhpal
authors.initials: S
authors.lastname: Singh
authors.forename: Ilyas
authors.initials: I
authors.lastname: Ahmed
authors.forename: B
authors.initials: B
authors.lastname: Mishra
authors.forename: A K
authors.initials: AK
authors.lastname: Singh
authors.forename: C
authors.initials: C
authors.lastname: Kole
mesh_terms: D000483:Alleles; D056726:Genetic Association Studies; D005838:Genotype; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics*; D051479:Plant Infertility / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D011110:Polymorphism, Genetic; D012641:Selection, Genetic; D013223:Statistics as Topic
chemical_list: D010940:Plant Proteins