title: [Systemic oxidative stress induced by intratracheal instilling with PM10 in rats].
abstract: OBJECTIVE
  To observe the oxidative stress of serum, lung and heart tissues in rats caused by PM10.
  
  
  METHODS
  In the study,24 male Wistar rats weighed 320-360 g were randomly divided into four groups, including one saline control group and three PM10 exposed groups. PM10 was administered to the exposed groups by intratracheal instillation at the doses of 3.75, 7.5, 15 mg/kg, respectively. The rats were sacrificed 24 hours after exposure. The levels of MDA and activities of SOD in the serum, lung and heart tissues were measured. In addition, the contents of protein carbonyl in lung and heart tissues were determined.
  
  
  RESULTS
  The activities of SOD in the serum, lung and heart tissues decreased markedly in the 7.5 and 15 mg/kg groups. The levels of MDA in the lung tissues significantly increased in the 7.5 and 15 mg/kg groups, while those in the serum and heart tissues significantly increased only in the 15 mg/kg group. The contents of protein carbonyl in the lung tissues markedly increased in the 15 mg/kg group, but no significant change was found in the heart tissues.
  
  
  CONCLUSIONS
  The study showed that PM10 can cause oxidative stress effects in rats. The lipids of cell membrane are more susceptible to oxidative stress than the proteins.
authors.affiliation: Department of Occupational & Environmental Health Sciences, Peking University School of Public Health, Beijing 100191, China.
authors.forename: Xiu-ling
authors.initials: XL
authors.lastname: Lu
authors.forename: Xin-ru
authors.initials: XR
authors.lastname: Zhang
authors.forename: Fu-rong
authors.initials: FR
authors.lastname: Deng
authors.forename: Xin-biao
authors.initials: XB
authors.lastname: Guo
mesh_terms: D000280:Administration, Inhalation; D000393:Air Pollutants / Q000633:toxicity*; D000818:Animals; D004781:Environmental Exposure / Q000009:adverse effects; D006321:Heart / Q000503:physiopathology; D008168:Lung / Q000503:physiopathology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D052638:Particulate Matter / Q000633:toxicity*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000097:blood / Q000378:metabolism
chemical_list: D000393:Air Pollutants; D052638:Particulate Matter; D008315:Malondialdehyde; D013482:Superoxide Dismutase