title: Suppression of FAT/CD36 mRNA by human growth hormone in pancreatic β-cells.
abstract: Fatty acid-induced damage in pancreatic β-cells is assumed to play an important role in the development of type 2 diabetes. Lactogens (prolactin, placental lactogen and growth hormone) improve β-cell survival via STAT5 activation but the molecular targets are incompletely characterized. The aim of this study was to examine the effect of human growth hormone (hGH) on mRNAs of fatty acid transport and binding proteins expressed in pancreatic β-cells, and to examine this in relation to β-cell survival after exposure to fatty acids. hGH decreased mRNA levels of FAT/CD36, whereas mRNAs of GPR40, FASN, FABP2, FATP1 and FATP4 were unchanged. RNAi against FAT/CD36 decreased fatty acid-induced apoptosis. Over-expression of constitutively active STAT5 was able to mimic hGH's suppression of FAT/CD36 expression, whereas dominant negative STAT5 was unable to block the effect of hGH indicating that STAT5 did not bind directly to the FAT/CD36 promoter. The hGH-mediated suppression of FAT/CD36 mRNA was associated with a decrease in palmitate uptake and fatty acid-induced basal hyper-secretion of insulin resulting in improved glucose-stimulated insulin secretion. This study suggests that hGH can protect β-cells against fatty acid-induced damages.
authors.affiliation: Department of Biomedical Sciences, University of Copenhagen, Copenhagen, Denmark. ltd@ruc.dk
authors.forename: Louise T
authors.initials: LT
authors.lastname: Dalgaard
authors.forename: Peter
authors.initials: P
authors.lastname: Thams
authors.forename: Louise W
authors.initials: LW
authors.lastname: Gaarn
authors.forename: Janne
authors.initials: J
authors.lastname: Jensen
authors.forename: Ying C
authors.initials: YC
authors.lastname: Lee
authors.forename: Jens H
authors.initials: JH
authors.lastname: Nielsen
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D001692:Biological Transport; D018955:CD36 Antigens / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D019610:Cytoprotection; D005947:Glucose / Q000494:pharmacology; D019382:Human Growth Hormone / Q000494:pharmacology / Q000502:physiology*; D006801:Humans; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D050417:Insulin-Secreting Cells / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D010168:Palmitates / Q000378:metabolism* / Q000633:toxicity; D012333:RNA, Messenger / Q000037:antagonists & inhibitors / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D051381:Rats; D050799:STAT5 Transcription Factor / Q000378:metabolism
chemical_list: D018955:CD36 Antigens; D007328:Insulin; D010168:Palmitates; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D050799:STAT5 Transcription Factor; D019382:Human Growth Hormone; D005947:Glucose