title: The generation of iPS cells using non-viral magnetic nanoparticle based transfection.
abstract: Induced pluripotent stem (iPS) cells have been generated from various somatic cells; however, a major restriction of the technology is the use of potentially harmful genome-integrating viral DNAs. Here, without a viral vector, we generated iPS cells from fibroblasts using a non-viral magnetic nanoparticle-based transfection method that employs biodegradable cationic polymer PEI-coated super paramagnetic nanoparticles (NP). Our findings support the possible use of transient expression of iPS genes in somatic cells by magnet-based nanofection for efficient generation of iPS cells. Results of dynamic light scattering (DLS) analysis and TEM analyses demonstrated efficient conjugation of NP with iPS genes. After transfection, nanofection-mediated iPS cells showed ES cell-like characteristics, including expression of endogenous pluripotency genes, differentiation of three germ layer lineages, and formation of teratomas. Our results demonstrate that magnet-based nanofection may provide a safe method for use in generation of virus-free and exogenous DNA-free iPS cells, which will be crucial for future clinical applications in the field of regenerative medicine.
authors.affiliation: Department of Animal Biotechnology and Animal Resources Research Center, Konkuk University, Seoul, 143-701, Republic of Korea.
authors.forename: Chang Hyun
authors.initials: CH
authors.lastname: Lee
authors.forename: Jung-Hyun
authors.initials: JH
authors.lastname: Kim
authors.forename: Hyun Joo
authors.initials: HJ
authors.lastname: Lee
authors.forename: Kilsoo
authors.initials: K
authors.lastname: Jeon
authors.forename: HyeJin
authors.initials: H
authors.lastname: Lim
authors.forename: Hye yeon
authors.initials: Hy
authors.lastname: Choi
authors.forename: Eung-Ryoung
authors.initials: ER
authors.lastname: Lee
authors.forename: Seung Hwa
authors.initials: SH
authors.lastname: Park
authors.forename: Jae-Yong
authors.initials: JY
authors.lastname: Park
authors.forename: Sunghoi
authors.initials: S
authors.lastname: Hong
authors.forename: Soonhag
authors.initials: S
authors.lastname: Kim
authors.forename: Ssang-Goo
authors.initials: SG
authors.lastname: Cho
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D019070:Cell Lineage; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000502:physiology*; D008280:Magnetics / Q000379:methods*; D008422:Materials Testing; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D008810:Mice, Inbred C57BL; D014162:Transfection / Q000379:methods*