title: Altered expression of matrix metalloproteinases and their tissue inhibitors in matured rat adipocytes in vitro.
abstract: Obesity is recognized as a risk factor for delayed cutaneous wound healing. The authors hypothesized that the secretion of matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) from subcutaneous adipose tissue correlates with disorder of the healing process in obese subjects. Findings from previous studies on the expression of MMPs and TIMPs in obese adipose tissue are inconsistent. Since these conflicting results could be due to the effect of several intrinsic factors, the authors conducted a simple in vitro experiment to clarify the change in profile of MMPs and TIMPs in excessively matured adipocytes. The authors cultured the induced adipocytes under conditions of high or low glucose and with or without insulin supplementation. Oil red O staining and its dye extraction assay revealed excessive lipid accumulation in high glucose and insulin-supplemented adipocytes. Additionally, there was altered expression of adipokines, similar to the change in adipose tissue in obese subjects. Under these conditions, the expression/activity of MMP8 was promoted and the expression of MMP3 and TIMP3 was inhibited. Further studies to determine the effect of other obesity-related factors, such as insulin resistance, on MMPs and TIMPs are required.
authors.affiliation: Department of Advanced Skin Care, The University of Tokyo, Tokyo, Japan.
authors.forename: Takeo
authors.initials: T
authors.lastname: Minematsu
authors.forename: Lijuan
authors.initials: L
authors.lastname: Huang
authors.forename: Ai
authors.initials: A
authors.lastname: Ibuki
authors.forename: Gojiro
authors.initials: G
authors.lastname: Nakagami
authors.forename: Tomoko
authors.initials: T
authors.lastname: Akase
authors.forename: Junko
authors.initials: J
authors.lastname: Sugama
authors.forename: Takashi
authors.initials: T
authors.lastname: Nagase
authors.forename: Kotaro
authors.initials: K
authors.lastname: Yoshimura
authors.forename: Hiromi
authors.initials: H
authors.lastname: Sanada
mesh_terms: D017667:Adipocytes / Q000201:enzymology* / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D066298:In Vitro Techniques; D050356:Lipid Metabolism; D008297:Male; D020782:Matrix Metalloproteinases / Q000378:metabolism*; D059748:Proteolysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019714:Tissue Inhibitor of Metalloproteinases / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D019714:Tissue Inhibitor of Metalloproteinases; D020782:Matrix Metalloproteinases