title: Characterization of a hydrophobin of the ascomycete Paecilomyces farinosus.
abstract: The entomopathogenic ascomycete Paecilomyces farinosus (alternative name Isaria farinosa) synthesized a hydrophobin, irrespective of being grown in submerged or surface culture. The protein was extracted using trifluoroacetic acid and purified using preparative HPLC and SDS-PAGE. Partial sequences were obtained using ESI-MS/MS. The peptides were used as a start to apply a 'template switching oligo' protocol to elucidate the complete open reading frame of P. farinosus hydrophobin 1 (pfah1). The deduced protein sequence comprised 107 amino acids (10.7 kDa) including a 16 amino acid long hydrophobic signal peptide, showed a calculated pI of 4.56, and was interrupted by one intron. Phylogenetic analyses revealed relationships to hydrophobins of the ascomycetes Magnaporthe grisea and Metarhizium anisopliae. Based on solubility, hydropathy pattern and phylogeny PfaH1 was assigned to the class Ia hydrophobins.
authors.affiliation: Zentrum Angewandte Chemie der Leibniz Universität Hannover, Institut für Lebensmittelchemie, Callinstrasse 5, Hannover, Germany. stefan.lunkenbein@lci.uni-hannover.de
authors.forename: Stefan
authors.initials: S
authors.lastname: Lunkenbein
authors.forename: Meike
authors.initials: M
authors.lastname: Takenberg
authors.forename: Manfred
authors.initials: M
authors.lastname: Nimtz
authors.forename: Ralf G
authors.initials: RG
authors.lastname: Berger
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry; D018076:DNA, Complementary / Q000096:biosynthesis / Q000737:chemistry; D005656:Fungal Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D005800:Genes, Fungal / Q000235:genetics; D008969:Molecular Sequence Data; D010143:Paecilomyces / Q000737:chemistry* / Q000145:classification / Q000235:genetics / Q000254:growth & development; D010802:Phylogeny; D021382:Protein Sorting Signals / Q000235:genetics; D012329:RNA, Bacterial / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D018076:DNA, Complementary; D005656:Fungal Proteins; D021382:Protein Sorting Signals; D012329:RNA, Bacterial