title: [Effects of a novel borate bioglass on osteoblast behavior in vitro].
abstract: OBJECTIVE
  As a bioactive material, the osteogenic activity of borate bioglass has been proved. To design a novel borate bioglass according to an improved formula and to investigate the effects of the borate bioglass on osteoblasts in vitro for further research and potential clinical application.
  
  
  METHODS
  The novel Na2O-K2O-MgO-CaO-P205-B203-SrO borate bioglass was prepared by melting process. The initial and secondary extracts were prepared according to ISO10993-12: 2007 respectively with different extract time of 0-24 hours and 24-48 hours. The osteoblasts (MC3T3-E1) of the 5th-15th passages from mouse were cocultured with the initial (initial extract group) and secondary (secondary extract group) extracts, respectively, to assess the effects of the borate bioglass on the cell proliferation, protein synthesis, alkaline phosphatase (ALP) activity, cell apoptosis, and cell migration; while alpha-MEM medium without addition of extract served as control group.
  
  
  RESULTS
  The absorbance values at 450 nm were 0.3560 +/- 0.0187, 0.331 0 +/- 0.025 4, and 0.2040 +/- 0.0138 in initial extract, secondary extract, and control groups, respectively, showing significant differences among 3 groups (P < 0.05). The total protein contents were (382.847 +/- 9.521), (226.071 +/- 5.847), and (220.248 +/- 8.213) U in initial extract, secondary extract, and control groups, respectively; there were significant differences between initial extract group and control group, and between initial extract group and secondary group (P < 0.05), but there was no significant difference between secondary extract group and control group (P > 0.05). However, no significant difference was observed in the ALP activity [(0.01301 +/- 0.00039), (0.012 93 +/- 0.00044), and (0.012 92 +/- 0.000 35) U/mg], apoptosis rate (7.03% +/- 1.95%, 6.46% +/- 2.88%, and 6.18% +/- 2.21%), horizontal migration [(137.50 +/- 11.43), (134.98 +/- 10.50), (135.21 +/- 8.66) microm], and transmembrane cell number [(10.92 +/- 4.99), (10.07 +/- 2.50), and (9.81 +/- 2.64) cells/field] among initial extract, secondary extract, and control groups (P > 0.05).
  
  
  CONCLUSIONS
  This novel borate bioglass has excellent cytocompatibility, which plays regulatory effects on the cell proliferation, secretion, and migration.
authors.affiliation: Institution of Microsurgery, Affiliated Shanghai Sixth People's Hospital, Shanghai Jiaotong University, Shanghai, 200233, PR China.
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Wei
authors.forename: Changqing
authors.initials: C
authors.lastname: Zhang
authors.forename: Wenhai
authors.initials: W
authors.lastname: Huang
authors.forename: Yifei
authors.initials: Y
authors.lastname: Gu
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D001881:Borates / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D002516:Ceramics; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects*; D010012:Osteogenesis
chemical_list: D001672:Biocompatible Materials; C022107:Bioglass; D001881:Borates