title: Induction and flow cytometry identification of tetraploids from seed-derived explants through colchicine treatments in Catharanthus roseus (L.) G. Don.
abstract: The tetraploid plants of Catharanthus roseus (L.) G. Don was obtained by colchicine induction from seeds explants, and the ploidy of the plants was identified by flow cytometry. The optimal treatment is 0.2% colchicine solution treated for 24 hours, and the induction rate reaches up to 30%. Comparing with morphological characteristics and growth habits between tetraploids and the control, we found that tetraploids of C. roseus had larger stoma and more branches and leaves. HPLC analysis showed tetraploidization could increase the contents of terpenoid indole alkaloids in C. roseus. Thus, tetraploidization could be used to produce higher alkaloids lines for commercial use. QRT-PCR results showed that the expression of enzymes involved in terpenoid indole alkaloids biosynthesis pathway had increased in the tetraploid plants. To our knowledge, this was the first paper to explore the secondary metabolism in autotetraploid C. roseus induced by colchicine.
authors.affiliation: Plant Biotechnology Research Center, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Shi-Hai
authors.initials: SH
authors.lastname: Xing
authors.forename: Xin-Bo
authors.initials: XB
authors.lastname: Guo
authors.forename: Quan
authors.initials: Q
authors.lastname: Wang
authors.forename: Qi-Fang
authors.initials: QF
authors.lastname: Pan
authors.forename: Yue-Sheng
authors.initials: YS
authors.lastname: Tian
authors.forename: Pin
authors.initials: P
authors.lastname: Liu
authors.forename: Jing-Ya
authors.initials: JY
authors.lastname: Zhao
authors.forename: Guo-Feng
authors.initials: GF
authors.lastname: Wang
authors.forename: Xiao-Fen
authors.initials: XF
authors.lastname: Sun
authors.forename: Ke-Xuan
authors.initials: KX
authors.lastname: Tang
mesh_terms: D000704:Analysis of Variance; D030008:Catharanthus / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism; D003078:Colchicine / Q000494:pharmacology*; D005434:Flow Cytometry / Q000379:methods*; D015870:Gene Expression; D010641:Phenotype; D010936:Plant Extracts / Q000302:isolation & purification / Q000378:metabolism; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D046948:Secologanin Tryptamine Alkaloids / Q000302:isolation & purification* / Q000378:metabolism*; D012639:Seeds / Q000235:genetics* / Q000378:metabolism; D057891:Tetraploidy
chemical_list: D010936:Plant Extracts; D046948:Secologanin Tryptamine Alkaloids; D003078:Colchicine