title: Impedance analysis of adherent cells after in situ electroporation: non-invasive monitoring during intracellular manipulations.
abstract: In this study adherent animal cells were grown to confluence on circular gold-film electrodes of 250 μm diameter that had been deposited on the surface of a regular culture dish. The impedance of the cell-covered electrode was measured at designated frequencies to monitor the behavior of the cells with time. This approach is referred to as electric cell-substrate impedance sensing or short ECIS in the literature. The gold-film electrodes were also used to deliver well-defined AC voltage pulses of several volts amplitude and several hundred milliseconds duration to the adherent cells in order to achieve reversible membrane electroporation (in situ electroporation=ISE). Electroporation-assisted introduction of membrane impermeable molecules into the cytoplasm was studied by using FITC-labeled dextran molecules of different molecular weights. Probes as big as 2MDa were successfully loaded into the cells residing on the electrode surface. Time-resolved impedance measurements before and immediately after the electroporation pulse revealed the kinetics of membrane resealing as well as subsequent changes in cell morphology. Cells recovered from the electroporation pulse within less than 90 min. When membrane-impermeable, bioactive compounds like N(3)(-) or bleomycin were introduced into the cells by in situ electroporation, concomitant ECIS readings sensitively reported on the associated response of the cells to these toxins as a function of time (ISE-ECIS).
authors.affiliation: Institut fuer Analytische Chemie, Chemo- & Biosensorik, Universitaet Regensburg, Universitaetsstr. 31, 93053 Regensburg, Germany.
authors.forename: Judith A
authors.initials: JA
authors.lastname: Stolwijk
authors.forename: Christoph
authors.initials: C
authors.lastname: Hartmann
authors.forename: Poonam
authors.initials: P
authors.lastname: Balani
authors.forename: Silke
authors.initials: S
authors.lastname: Albermann
authors.forename: Charles R
authors.initials: CR
authors.lastname: Keese
authors.forename: Ivar
authors.initials: I
authors.lastname: Giaever
authors.forename: Joachim
authors.initials: J
authors.lastname: Wegener
mesh_terms: D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage*; D001761:Bleomycin / Q000008:administration & dosage*; D002448:Cell Adhesion; D002460:Cell Line; D017097:Electric Impedance; D018274:Electroporation / Q000295:instrumentation*; D004867:Equipment Design; D005456:Fluorescent Dyes / Q000008:administration & dosage*; D006801:Humans; D007700:Kinetics
chemical_list: D000903:Antibiotics, Antineoplastic; D005456:Fluorescent Dyes; D001761:Bleomycin