title: Preparation and characterization of bioactive mesoporous calcium silicate-silk fibroin composite films.
abstract: Composite films of bioactive mesoporous calcium silicate (MCS)/silk fibroin (SF) and conventional calcium silicate (CS)/SF were fabricated by the solvent casting method, and the structures and properties of the composite films were characterized. Results of field emission scanning electron microscope (FESEM) indicated that MCS or CS was uniformly dispersed in the SF films. The measurements of the water contact angles suggested that the incorporation of either MCS or CS into SF could improve the hydrophilicity of the composite films, and the former was more effective than the later. The bioactivity of the composite films was evaluated by soaking in a simulated body fluid (SBF), and the formation of a hydroxycarbonate apatite (HCA) layer was determined by XRD and FT-IR. The results showed that the MCS/SF composite films have significantly enhanced apatite-forming bioactivity compared with the CS/SF composite films owing to the highly specific surface area and pore volume of MCS. In vitro cell attachment and proliferation tests showed that the MCS/SF composite film was a good matrix for the growth of MG63 cells. Consequently, the MCS/SF composite film possessed excellent physicochemical and biological properties, indicating its potential application for bone tissue engineering by designing 3D scaffolds according to its corresponding composition.
authors.affiliation: The Key Laboratory of Advanced Textile Materials and Manufacturing Technology, Xiasha Higher Education Zone, Zhejiang Sci-Tech University, Hangzhou, 310018, China.
authors.forename: Hailin
authors.initials: H
authors.lastname: Zhu
authors.forename: Binwei
authors.initials: B
authors.lastname: Wu
authors.forename: Xinxing
authors.initials: X
authors.lastname: Feng
authors.forename: Jianyong
authors.initials: J
authors.lastname: Chen
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D001842:Bone and Bones / Q000166:cytology*; D017610:Calcium Compounds; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D005349:Fibroins / Q000737:chemistry / Q000627:therapeutic use*; D006801:Humans; D017640:Silicates / Q000737:chemistry / Q000627:therapeutic use*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D017610:Calcium Compounds; D017640:Silicates; D005349:Fibroins