title: [A cell-based detection of ciguatoxin using sodium fluorescence probe].
abstract: OBJECTIVE
  To establish a cell-based detection method of ciguatoxin using fluorescence assay.
  
  
  METHODS
  Mouse neuroblastoma N-2A cells were exposed to ouabain and veratridine and different concentrations of standard ciguatoxin samples (P-CTX-1) to establish the curvilinear relationship between the toxin dosage and fluorescence intensity using the sodium fluorescence probe CoroNaTM Green. The toxicity curvilinear relationship was also generated between the toxin dosage and cell survival using CCK-8 method. Based on these standard curves, the presence of ciguatoxin was detected in 33 samples of deep-sea coral fish.
  
  
  RESULTS
  A correlation was found between the detection results of cell-based fluorescence assay and cytotoxicity assay, whose detection limit reached 103 g/ml and 1012 g/ml, respectively. The cell-based fluorescent assay sensitivity showed a higher sensitivity than cytotoxicity assay with a 2-4 h reduction of the detection time.
  
  
  CONCLUSIONS
  The cell-based fluorescent assay can quickly and sensitively detect ciguatoxin and may serve as a good option for preliminary screening of the toxin.
authors.affiliation: Key Laboratory of Modern Toxicology in Shenzhen, Shenzhen Center for Disease Control and Prevention, Shenzhen 518020, China. jianhui_yuan@126.com
authors.forename: Jian-hui
authors.initials: JH
authors.lastname: Yuan
authors.forename: Hui
authors.initials: H
authors.lastname: Yang
authors.forename: Huan-wen
authors.initials: HW
authors.lastname: Tang
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.forename: Xin-yun
authors.initials: XY
authors.lastname: Xu
authors.forename: Jian-jun
authors.initials: JJ
authors.lastname: Liu
authors.forename: Yue-bin
authors.initials: YB
authors.lastname: Ke
authors.forename: Jin-quan
authors.initials: JQ
authors.lastname: Cheng
authors.forename: Zhi-xiong
authors.initials: ZX
authors.lastname: Zhuang
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002922:Ciguatoxins / Q000633:toxicity*; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods*; D005399:Fishes; D005456:Fluorescent Dyes; D051379:Mice; D012964:Sodium
chemical_list: D005456:Fluorescent Dyes; D002922:Ciguatoxins; D012964:Sodium