title: Characterization of enterotoxigenic E. coli (ETEC), Shiga-toxin producing E. coli (STEC) and necrotoxigenic E. coli (NTEC) isolated from diarrhoeic Mediterranean water buffalo calves (Bubalus bubalis).
abstract: Two hundred and twenty Escherichia coli isolates from 314 Mediterranean water buffalo calves less than 4 weeks old affected by severe diarrhoea with a lethal outcome were characterized for the presence of the virulence factors LT, ST, Stx1, Stx2, haemolysins, intimin, CNF1, CNF2, CDT-I, CDT-II, CDT-III, CDT-IV, and F17-related fimbriae (F17a, F17b, F17c, F17d). The prevalence of ETEC, STEC and NTEC were 1.8%, 6.8% and 20.9%, respectively. The ETEC isolates were all LT-positive and ST-negative. The STEC isolates were all Stx and intimin-positive, with Stx1 (80%) more frequent than Stx2 (27%). The NTEC isolates were all CNF and Hly-positive, with CNF2 (83%) more frequent than CNF1 (22%). Susceptibility assays to 11 antimicrobials displayed high rates of resistance (>30%) to antimicrobials tested. These data show that the most prevalent strains in diarrhoeic water buffalo calves were NTEC, mostly CNF2 and HlyA-positive, with strong associations CNF2/CDT-III and CNF2/F17c.
authors.affiliation: Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute, 2, 80055 Portici, NA, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Borriello
authors.forename: M G
authors.initials: MG
authors.lastname: Lucibelli
authors.forename: E
authors.initials: E
authors.lastname: De Carlo
authors.forename: C
authors.initials: C
authors.lastname: Auriemma
authors.forename: D
authors.initials: D
authors.lastname: Cozza
authors.forename: G
authors.initials: G
authors.lastname: Ascione
authors.forename: F
authors.initials: F
authors.lastname: Scognamiglio
authors.forename: G
authors.initials: G
authors.lastname: Iovane
authors.forename: G
authors.initials: G
authors.lastname: Galiero
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000382:microbiology; D000890:Anti-Infective Agents / Q000627:therapeutic use; D002020:Buffaloes / Q000382:microbiology*; D003967:Diarrhea / Q000188:drug therapy / Q000209:etiology / Q000382:microbiology / Q000662:veterinary*; D024881:Drug Resistance, Bacterial; D054307:Enterotoxigenic Escherichia coli / Q000187:drug effects / Q000302:isolation & purification / Q000472:pathogenicity*; D004926:Escherichia coli / Q000187:drug effects / Q000302:isolation & purification / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000150:complications / Q000188:drug therapy / Q000382:microbiology / Q000662:veterinary*; D008826:Microbial Sensitivity Tests; D054323:Shiga-Toxigenic Escherichia coli / Q000187:drug effects / Q000302:isolation & purification / Q000472:pathogenicity*; D037521:Virulence Factors / Q000032:analysis
chemical_list: D000890:Anti-Infective Agents; D037521:Virulence Factors