title: A probe-based method for confirmation of methicillin-resistant Staphylococcus aureus and detection of Panton-Valentine leukocidin and tst virulence genes.
abstract: Probe-based detection of mecA, lukS/F-PV (Panton-Valentine leukocidin), and tst virulence genes in 435 isolates of Staphylococcus aureus had comparable sensitivity and specificity to end-point polymerase chain reaction as a reference standard.
authors.affiliation: St. Jude Children's Research Hospital and the University of Tennessee, Memphis, TN, USA. ashok.srinivasan@stjude.org
authors.forename: Ashok
authors.initials: A
authors.lastname: Srinivasan
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Bankowski
authors.forename: Steven E
authors.initials: SE
authors.lastname: Seifried
authors.forename: Sadao
authors.initials: S
authors.lastname: Jinno
authors.forename: Rosalie
authors.initials: R
authors.lastname: Perkins
authors.forename: Seema
authors.initials: S
authors.lastname: Singh
authors.forename: Claire
authors.initials: C
authors.lastname: Ying
authors.forename: Alan D
authors.initials: AD
authors.lastname: Tice
authors.forename: Wesley
authors.initials: W
authors.lastname: Kim
authors.forename: Randall T
authors.initials: RT
authors.lastname: Hayden
mesh_terms: D001427:Bacterial Toxins / Q000235:genetics*; D004768:Enterotoxins / Q000235:genetics*; D005098:Exotoxins / Q000235:genetics*; D006801:Humans; D007956:Leukocidins / Q000235:genetics*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000235:genetics* / Q000302:isolation & purification*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D015345:Oligonucleotide Probes / Q000235:genetics; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000382:microbiology*; D018089:Superantigens / Q000235:genetics*; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D001427:Bacterial Toxins; D004768:Enterotoxins; D005098:Exotoxins; D007956:Leukocidins; D015345:Oligonucleotide Probes; C078284:Panton-Valentine leukocidin; D018089:Superantigens; D037521:Virulence Factors; C030107:enterotoxin F, Staphylococcal