title: Histological and immunohistochemical evaluation of the peri-implant soft tissues around machined and acid-etched titanium healing abutments: a prospective randomised study.
abstract: A close spatial correlation has been described between the roughness of intraoral materials and the rate of bacterial colonisation. The aim of the present study in man was to conduct a comparative immunohistochemical evaluation of the inflammatory infiltrate, microvessel density, the nitric oxide synthases 1 and 3 and the vascular endothelial growth factor expression, the proliferative activity, and the B and T lymphocyte and histiocyte positivity in the peri-implant soft tissues around machined and acid-etched titanium healing caps. Ten patients participated in this study. The patients were enrolled consecutively. All patients received dental implants left to heal in a non-submerged mode. Healing caps were inserted in all implants. Half of the implants were supplied randomly with machined caps of titanium (control), while the other half were provided randomly with acid-etched titanium caps (test). After a 6-month healing period, a gingival biopsy was performed with a circular scalpel around the healing caps of both groups. The inflammatory infiltrate was mostly present in test specimens. Their extension was much larger than that of the control samples. A higher number of T and B lymphocytes were observed in test specimens. Higher values of microvessel density and a higher expression of vascular endothelial growth factor intensity were observed in the test samples. Furthermore, the Ki-67, NOS1 and NOS3 expression was significantly higher in the test specimens. All these results showed that the tissues around test healing caps underwent a higher rate of restorative processes, most probably correlated to the higher inflammation processes observed in these tissues.
authors.affiliation: Dental School, University of Chieti-Pescara, via dei Vestini 31, 66100 Chieti, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Degidi
authors.forename: Luciano
authors.initials: L
authors.lastname: Artese
authors.forename: Adriano
authors.initials: A
authors.lastname: Piattelli
authors.forename: Antonio
authors.initials: A
authors.lastname: Scarano
authors.forename: Jamil A
authors.initials: JA
authors.lastname: Shibli
authors.forename: Marcello
authors.initials: M
authors.lastname: Piccirilli
authors.forename: Vittoria
authors.initials: V
authors.lastname: Perrotti
authors.forename: Giovanna
authors.initials: G
authors.lastname: Iezzi
mesh_terms: D000134:Acid Etching, Dental / Q000009:adverse effects*; D000328:Adult; D000368:Aged; D000044:Dental Abutments / Q000009:adverse effects*; D015921:Dental Implants; D003775:Dental Polishing; D005260:Female; D005881:Gingiva / Q000098:blood supply / Q000276:immunology / Q000378:metabolism*; D006644:Histiocytes / Q000276:immunology; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism; D007527:Isoenzymes / Q000096:biosynthesis; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D055806:Microvessels; D008875:Middle Aged; D019001:Nitric Oxide Synthase / Q000096:biosynthesis; D057873:Peri-Implantitis / Q000209:etiology* / Q000276:immunology / Q000378:metabolism; D011446:Prospective Studies; D016037:Single-Blind Method; D013499:Surface Properties; D014025:Titanium; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis; D014945:Wound Healing
chemical_list: D015921:Dental Implants; D018836:Inflammation Mediators; D007527:Isoenzymes; D042461:Vascular Endothelial Growth Factor A; D014025:Titanium; D019001:Nitric Oxide Synthase