title: Differential expression of Mart-1 in human uveal melanoma cells.
abstract: Uveal melanoma (UM) is one of the most common primary intraocular malignant tumors in adults. Melanoma antigen recognized by T cell-1 (Mart-1), one of the melanosome-specific proteins, has been widely studied as a marker recognized by cytotoxicity T lymphocytes. Mart-1 is considered to play a critical role in the immunotherapy for melanoma. Additionally, as a biomarker, Mart-1 is often used with other tumor-associated antigens for antidiastole in cutaneous melanoma (CM), uveal melanoma (UM) and nevus. In this study, the differential expression of Mart-1 was investigated in four human UM cells (SP6.5, VUP, OCM-1 and OM431) on three levels of analysis: messenger ribonucleic acid (mRNA), protein and, eventually, morphology. The results revealed that SP6.5 cells had high Mart-1 protein expression while VUP cells had almost none. OCM-1 and OM431 cells produced less Mart-1 than SP6.5 cells according to Western blot analysis, although OM431 cells had the highest expression of Mart-1 mRNA according to real-time PCR. The results indicate the potential use of Mart-1 in the development of therapy for UM.
authors.affiliation: Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiaotong University, School of Medicine, Shanghai 200011, P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Renbing
authors.initials: R
authors.lastname: Jia
authors.forename: Yuting
authors.initials: Y
authors.lastname: Yao
authors.forename: Biyun
authors.initials: B
authors.lastname: Cun
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Huang
authors.forename: Ping
authors.initials: P
authors.lastname: Gu
authors.forename: Shengfang
authors.initials: S
authors.lastname: Ge
authors.forename: Xianqun
authors.initials: X
authors.lastname: Fan
mesh_terms: D045744:Cell Line, Tumor; D005455:Fluorescent Antibody Technique; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D058965:MART-1 Antigen / Q000235:genetics* / Q000378:metabolism; D008545:Melanoma / Q000235:genetics* / Q000473:pathology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014604:Uveal Neoplasms / Q000235:genetics* / Q000473:pathology*; D000098943:Uveal Melanoma
chemical_list: D058965:MART-1 Antigen; C548667:MLANA protein, human; D012333:RNA, Messenger