title: A screen for peptide agonists of the G-CSF receptor.
abstract: BACKGROUND
  Granulocyte-colony stimulating factor (G-CSF) is one of the most important pharmacologically used proteins. Potential uses beyond the stimulation of neutrophilic granulocytes are the treatment of CNS disorders. Disadvantages of the G-CSF protein as a drug are its moderate plasma half-life time and considerable production costs. We therefore conducted a screen for peptide agonists derived from the sequence of human G-CSF.
  
  
  RESULTS
  Despite of the high sensitivity of our screening system we could not detect any positive hits in a single peptide approach. In a multiplex approach using a permutation of any combination of 10 different peptides we could also not detect a positive block.
  
  
  CONCLUSIONS
  We conclude that larger coherent parts of the protein or dimerising peptides may be needed to achieve activation of the receptor.
authors.affiliation: SYGNIS Bioscience, Im Neuenheimer Feld 515, 69120 Heidelberg, Germany. schneider@sygnis.de.
authors.forename: Nadine
authors.initials: N
authors.lastname: Conzelmann
authors.forename: Armin
authors.initials: A
authors.lastname: Schneider