title: Characterization of Dp71Δ(78-79), a novel dystrophin mutant that stimulates PC12 cell differentiation.
abstract: Dp71 has an important role in the central nervous system. To better understand the function of Dp71 domains in neuronal differentiation, PC12 cells were stably transfected with a dystrophin mutant, Dp71Δ(78-79) , which lacks exons 78 and 79. Based on the percentage of cells bearing neurites and neurite length analyses, we found that cells stably expressing Dp71Δ(78-79) (PC12-C11) differentiate more efficiently than non-transfected cells. While wild-type cells reach their maximum differentiation 9-12 days after initiating the differentiation process, the PC12-C11 cells reach differentiation in 4-6 days. Protein expression analysis showed a down-regulation of Dp71a and an up-regulation of Dp71ab and/or Up71, β-dystroglycan and neuron-specific enolase in undifferentiated and in neural growth factor differentiated PC12-C11 cells. No change was observed in the expression of Grb2 and Up400. The subcellular localization of Dp71Δ(78-79) was in the cell periphery, and there was no change in localization during the differentiation process, which was also observed throughout the neurite extensions.
authors.affiliation: Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, México, DF, México.
authors.forename: Jorge
authors.initials: J
authors.lastname: Aragón
authors.forename: José
authors.initials: J
authors.lastname: Romo-Yáñez
authors.forename: Alejandro
authors.initials: A
authors.lastname: Martínez-Herrera
authors.forename: Víctor
authors.initials: V
authors.lastname: Ceja
authors.forename: Alvaro
authors.initials: A
authors.lastname: Rendon
authors.forename: Cecilia
authors.initials: C
authors.lastname: Montañez
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics*; D049030:Dystroglycans / Q000235:genetics / Q000378:metabolism; D016189:Dystrophin / Q000235:genetics*; D005091:Exons / Q000235:genetics; D051380:GRB2 Adaptor Protein / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects / Q000235:genetics*; D009154:Mutation / Q000235:genetics*; D020932:Nerve Growth Factor / Q000494:pharmacology; D016501:Neurites; D016716:PC12 Cells / Q000502:physiology; D010751:Phosphopyruvate Hydratase / Q000235:genetics / Q000378:metabolism; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors; D014162:Transfection / Q000379:methods
chemical_list: D016189:Dystrophin; D051380:GRB2 Adaptor Protein; C495950:Grb2 protein, rat; D020033:Protein Isoforms; D012333:RNA, Messenger; D011994:Recombinant Proteins; C085339:apo-dystrophin 1; D049030:Dystroglycans; D020932:Nerve Growth Factor; D010751:Phosphopyruvate Hydratase