title: Chronic toxicity of pesticides to the mRNA expression levels of metallothioneins and cytochrome P450 1A genes in rainbow trout.
abstract: The hazardous effects of pesticides on various metabolic pathways are a great problem for environmental health and should be well determined. In the present study, the authors treated rainbow trout with 0.6 μg/L deltamethrin for 28 days and 1.6 mg/L 2,2-dichlorovinyl dimethyl phosphate for 21 days. After this time period, the authors observed alterations in mRNA expression levels of MT-A, MT-B and CYP-1A. Chronic exposure to low levels of pesticides may have a more significant effect on fish populations than acute poisoning. While both pesticides caused a significant increase on mRNA levels of MT-A and CYP-1A, MT-B mRNA levels were increased significantly only upon deltamethin administration. The significant increase in mRNA levels of the corresponding genes may be considered as a defence mechanism in addition to the antioxidants against oxidative stress, as well as a detoxification mechanism against adverse effects of pesticides.
authors.affiliation: Hinis Vocational Training School, Atatürk University, Erzurum, Turkey. saltukceyhun@hotmail.com
authors.forename: Saltuk Bugrahan
authors.initials: SB
authors.lastname: Ceyhun
authors.forename: Ercüment
authors.initials: E
authors.lastname: Aksakal
authors.forename: Birsen
authors.initials: B
authors.lastname: Kirim
authors.forename: Kübra
authors.initials: K
authors.lastname: Atabeyoglu
authors.forename: Orhan
authors.initials: O
authors.lastname: Erdogan
mesh_terms: D000818:Animals; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics* / Q000378:metabolism; D004006:Dichlorvos / Q000633:toxicity; D015870:Gene Expression / Q000187:drug effects; D020869:Gene Expression Profiling; D007306:Insecticides / Q000633:toxicity*; D008668:Metallothionein / Q000235:genetics* / Q000378:metabolism; D009570:Nitriles / Q000633:toxicity; D017686:Oncorhynchus mykiss / Q000235:genetics / Q000378:metabolism*; D011722:Pyrethrins / Q000633:toxicity; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D036401:Toxicity Tests, Chronic
chemical_list: D007306:Insecticides; D009570:Nitriles; D011722:Pyrethrins; D012333:RNA, Messenger; C017180:decamethrin; D004006:Dichlorvos; D008668:Metallothionein; D019363:Cytochrome P-450 CYP1A1