title: Decellularized bovine reinforced vessels for small-diameter tissue-engineered vascular grafts.
abstract: The aim of the present study was to investigate the influence of a decellularization protocol on the structure and the mechanical behavior of small-diameter (<6 mm) tibial calf arteries and veins. Calf vessels were decellularized by a detergent-enzymatic method (DEM), partially hydrolyzed with trypsin and subsequently cross-linked using poly(ethylene glycol) diglycidyl ether. Our results showed that i) the DEM can be considered a simple and valuable procedure for the preparation of complete acellular arteries and veins able to preserve a high degree of collagen and elastic fibers, and ii) poly(ethylene glycol) diglycidyl ether cross-linking treatment provides appropriate mechanical reinforcement of blood vessels. Histologically, the decellularized vessels were obtained employing the detergent-enzymatic procedure and their native extracellular matrix histoarchitecture and components remained well preserved. Moreover, the decellularization protocol can be considered an effective method to remove HLA class I antigen expression from small-diameter tibial calf arteries and veins. Cytocompatibility of decellularized cross-linked vessels was evaluated by endothelial and smooth muscle cell seeding on luminal and adventitial vessel surfaces, respectively.
authors.affiliation: Department of Pharmaceutical Sciences, University of Padua, Via Marzolo 5, I-35131 Padua, Italy. claudio.grandi@unipd.it
authors.forename: Claudio
authors.initials: C
authors.lastname: Grandi
authors.forename: Silvia
authors.initials: S
authors.lastname: Baiguera
authors.forename: Francesca
authors.initials: F
authors.lastname: Martorina
authors.forename: Silvano
authors.initials: S
authors.lastname: Lora
authors.forename: Pietro
authors.initials: P
authors.lastname: Amistà
authors.forename: Daniele
authors.initials: D
authors.lastname: Dalzoppo
authors.forename: Costantino
authors.initials: C
authors.lastname: Del Gaudio
authors.forename: Alessandra
authors.initials: A
authors.lastname: Bianco
authors.forename: Rosa
authors.initials: R
authors.lastname: Di Liddo
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Conconi
authors.forename: Pier Paolo
authors.initials: PP
authors.lastname: Parnigotto
mesh_terms: D000818:Animals; D001807:Blood Vessel Prosthesis; D001808:Blood Vessels / Q000166:cytology / Q000637:transplantation*; D002417:Cattle; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D003432:Cross-Linking Reagents / Q000378:metabolism; D042783:Endothelial Cells / Q000166:cytology; D004853:Epoxy Resins / Q000378:metabolism; D005998:Glycine / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008855:Microscopy, Electron, Scanning; D032389:Myocytes, Smooth Muscle / Q000166:cytology; D023822:Tissue Engineering / Q000379:methods*; D014357:Trypsin / Q000378:metabolism
chemical_list: D003432:Cross-Linking Reagents; D004853:Epoxy Resins; C035364:Quetol 651; D003094:Collagen; D014357:Trypsin; D005998:Glycine