title: Dectin-1 mediates in vitro phagocytosis of Candida albicans yeast cells by retinal microglia.
abstract: We have investigated the expression of TLR2 and Dectin-1 in retinal microglia and their involvement in Candida albicans phagocytosis using a cytometric approach. The expression of both receptors has been demonstrated in CD11b(+) retinal cells. Phagocytosis of pHrodo-labelled C. albicans yeasts by microglial CD11b(+) cells of C57BL/6 mice was inhibited both by the Dectin-1 antagonist laminarin and anti-Dectin-1 antibodies, whereas phagocytosis of yeasts by retinal microglia of TLR2 KO mice was unaffected. These data indicate that phagocytosis of C. albicans yeasts by retinal microglia is mediated by Dectin-1, whereas TLR2 does not play a significant role in this process.
authors.affiliation: Departamento de Óptica, Farmacología y Anatomía, Universidad de Alicante, San Vicente del Raspeig, Spain.
authors.forename: Victoria
authors.initials: V
authors.lastname: Maneu
authors.forename: Alberto
authors.initials: A
authors.lastname: Yáñez
authors.forename: Celia
authors.initials: C
authors.lastname: Murciano
authors.forename: Andrés
authors.initials: A
authors.lastname: Molina
authors.forename: María Luisa
authors.initials: ML
authors.lastname: Gil
authors.forename: Daniel
authors.initials: D
authors.lastname: Gozalbo
mesh_terms: D000818:Animals; D018845:CD11 Antigens / Q000032:analysis; D002176:Candida albicans / Q000276:immunology*; D020869:Gene Expression Profiling; D037181:Lectins, C-Type; D008565:Membrane Proteins / Q000096:biosynthesis / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D017628:Microglia / Q000737:chemistry / Q000276:immunology* / Q000382:microbiology; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000276:immunology*; D010587:Phagocytosis; D012160:Retina / Q000276:immunology* / Q000382:microbiology; D051195:Toll-Like Receptor 2 / Q000096:biosynthesis / Q000276:immunology; D000099181:Dectin-1
chemical_list: D018845:CD11 Antigens; D037181:Lectins, C-Type; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D051195:Toll-Like Receptor 2; D000099181:Dectin-1; C493486:Tlr2 protein, mouse