title: MiR-106a inhibits glioma cell growth by targeting E2F1 independent of p53 status.
abstract: MicroRNAs are single-stranded small non-coding RNA molecules which regulate mammalian cell growth, differentiation, and apoptosis by altering the expression of other genes and play a role in tumor genesis and progression. MiR-106a is upregulated in several types of malignancies and provides a pro-tumorigenic effect. However, its role in glioma is largely unknown. Our findings demonstrate that the low expression of miR-106a in human glioma specimens is significantly correlated with high levels of E2F1 protein and high-grade glioma. Here, we present the first evidence that miR-106a provides a tumor-suppressive effect via suppressing proliferation of and inducing apoptosis in human glioma cells. We further show that E2F1 is a direct functional target of miR-106a, suggesting that the effect of miR-106a on the glioma suppressive effect may result from inhibition of E2F1 via post-transcriptional regulation. In addition, our results reveal that miR-106a can increase p53 expression via E2F1 inhibition, whereas the effect of miR-106a on the proliferation of glioma cells is independent of p53 status. Further investigations will focus on the therapeutic use of miR-106a-mediated antitumor effects in glioma.
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, 150001, People's Republic of China.
authors.forename: Guang
authors.initials: G
authors.lastname: Yang
authors.forename: Ruyou
authors.initials: R
authors.lastname: Zhang
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Chen
authors.forename: Yulong
authors.initials: Y
authors.lastname: Mu
authors.forename: Jing
authors.initials: J
authors.lastname: Ai
authors.forename: Chen
authors.initials: C
authors.lastname: Shi
authors.forename: Yaohua
authors.initials: Y
authors.lastname: Liu
authors.forename: Changbin
authors.initials: C
authors.lastname: Shi
authors.forename: Lihua
authors.initials: L
authors.lastname: Sun
authors.forename: Nikolai G
authors.initials: NG
authors.lastname: Rainov
authors.forename: Hulun
authors.initials: H
authors.lastname: Li
authors.forename: Baofeng
authors.initials: B
authors.lastname: Yang
authors.forename: Shiguang
authors.initials: S
authors.lastname: Zhao
mesh_terms: D017209:Apoptosis / Q000235:genetics; D001483:Base Sequence; D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D050687:E2F1 Transcription Factor / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000235:genetics* / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D050505:Mutant Proteins / Q000378:metabolism; D060787:Neoplasm Grading; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: D050687:E2F1 Transcription Factor; C493753:E2F1 protein, human; C542541:MIRN106 microRNA, human; D035683:MicroRNAs; D050505:Mutant Proteins; D016159:Tumor Suppressor Protein p53