title: Idiopathic hirsutism: local and peripheral expression of aromatase (CYP19A) and 5α-reductase genes (SRD5A1 and SRD5A2).
abstract: OBJECTIVE
  To evaluate idiopathic hirsutism etiology via molecular studies testing peripheral and local aromatase and 5α-reductase expression.
  
  
  METHODS
  Assessment of the expression of messenger RNA (mRNA) for type 1 and 2,5α-reductase isoenzyme gene (SDR5A1, SDR5A2) and aromatase (CYP19A) in dermal papillae cells and peripheral blood mononuclear cells.
  
  
  METHODS
  University hospital.
  
  
  METHODS
  28 untreated idiopathic hirsute patients and 20 healthy women (controls).
  
  
  METHODS
  Human skin biopsies and peripheral venous blood.
  
  
  METHODS
  SDR5A1, SDR5A2, CYP19A gene expression in skin biopsies and peripheral blood.
  
  
  RESULTS
  A statistically significant reduction of SRD5A1, SRD5A2, and CYP19A gene expression was found in the dermal papillae cells and peripheral blood mononuclear cell between the study and control group.
  
  
  CONCLUSIONS
  Further study, including protein expression and enzyme activity assays, are warranted to characterize the paradoxically low gene expression levels of local 5α-reductase and aromatase in women with idiopathic hirsutism.
authors.affiliation: Department of Medical Genetics, School of Medicine, Erciyes University, Kayseri, Turkey.
authors.forename: A Okay
authors.initials: AO
authors.lastname: Caglayan
authors.forename: Munis
authors.initials: M
authors.lastname: Dundar
authors.forename: Fatih
authors.initials: F
authors.lastname: Tanriverdi
authors.forename: Nuran A
authors.initials: NA
authors.lastname: Baysal
authors.forename: Kursad
authors.initials: K
authors.lastname: Unluhizarci
authors.forename: Yusuf
authors.initials: Y
authors.lastname: Ozkul
authors.forename: Murat
authors.initials: M
authors.lastname: Borlu
authors.forename: Cem
authors.initials: C
authors.lastname: Batukan
authors.forename: Fahrettin
authors.initials: F
authors.lastname: Kelestimur
mesh_terms: D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase / Q000235:genetics*; D000293:Adolescent; D000328:Adult; D001141:Aromatase / Q000235:genetics*; D001706:Biopsy; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D020405:Dermis / Q000201:enzymology*; D015536:Down-Regulation; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D006628:Hirsutism / Q000097:blood / Q000201:enzymology / Q000235:genetics*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000201:enzymology*; D008565:Membrane Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis / Q000097:blood; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014421:Turkey; D055815:Young Adult
chemical_list: D008565:Membrane Proteins; D012333:RNA, Messenger; D001141:Aromatase; D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase; C548467:SRD5A1 protein, human; C548468:SRD5A2 protein, human