title: Lysinibacillus sphaericus S-layer protein toxicity against Culex quinquefasciatus.
abstract: The main toxicity mechanism of Lysinibacillus sphaericus, which is used in the control of mosquitoes, is its binary toxin produced during sporulation; additionally the Mtx1, Mtx2 and Mtx 3 toxins are expressed in vegetative cells. Mosquito larvicidal potency of the S-layer protein that is expressed in vegetative cells has been determined. The protein is similar to other S-layer proteins of mosquitocidal L. sphaericus strains. The LC50 values of the S-layer protein of the L. sphaericus OT4b25, OT4b26, and III(3)7 strains against third-instar larvae of Culex quinquefasciatus were 8.7, 24 and 0.68 μg/ml, respectively. To our knowledge this is the first study showing the mosquito larvicidal potency of the S-layer protein from Lysinibacillus sphaericus.
authors.affiliation: Centro de Investigaciones Microbiológicas-CIMIC, Departamento de Ciencias Biológicas, Universidad de los Andes, Cra 1E No. 18A-10 J207, Bogotá, Colombia. lulozano@uniandes.edu.co
authors.forename: Lucía C
authors.initials: LC
authors.lastname: Lozano
authors.forename: Juan A
authors.initials: JA
authors.lastname: Ayala
authors.forename: Jenny
authors.initials: J
authors.lastname: Dussán
mesh_terms: D000818:Animals; D001406:Bacillaceae / Q000737:chemistry* / Q000378:metabolism; D003465:Culex / Q000187:drug effects*; D004591:Electrophoresis, Polyacrylamide Gel; D007306:Insecticides / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D007814:Larva / Q000187:drug effects; D008562:Membrane Glycoproteins / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D020539:Sequence Analysis, Protein
chemical_list: D007306:Insecticides; D008562:Membrane Glycoproteins; C055764:S-layer proteins