title: Thermal properties of rhodopsin: insight into the molecular mechanism of dim-light vision.
abstract: Rhodopsin has developed mechanisms to optimize its sensitivity to light by suppressing dark noise and enhancing quantum yield. We propose that an intramolecular hydrogen-bonding network formed by ∼20 water molecules, the hydrophilic residues, and peptide backbones in the transmembrane region is essential to restrain thermal isomerization, the source of dark noise. We studied the thermal stability of rhodopsin at 55 °C with single point mutations (E181Q and S186A) that perturb the hydrogen-bonding network at the active site. We found that the rate of thermal isomerization increased by 1-2 orders of magnitude in the mutants. Our results illustrate the importance of the intact hydrogen-bonding network for dim-light detection, revealing the functional roles of water molecules in rhodopsin. We also show that thermal isomerization of 11-cis-retinal in solution can be catalyzed by wild-type opsin and that this catalytic property is not affected by the mutations. We characterize the catalytic effect and propose that it is due to steric interactions in the retinal-binding site and increases quantum yield by predetermining the trajectory of photoisomerization. Thus, our studies reveal a balancing act between dark noise and quantum yield, which have opposite effects on the thermal isomerization rate. The acquisition of the hydrogen-bonding network and the tuning of the steric interactions at the retinal-binding site are two important factors in the development of dim-light vision.
authors.affiliation: Department of Chemistry, Yale University, New Haven, Connecticut 06520, USA.
authors.forename: Jian
authors.initials: J
authors.lastname: Liu
authors.forename: Monica Yun
authors.initials: MY
authors.lastname: Liu
authors.forename: Jennifer B
authors.initials: JB
authors.lastname: Nguyen
authors.forename: Aditi
authors.initials: A
authors.lastname: Bhagat
authors.forename: Victoria
authors.initials: V
authors.lastname: Mooney
authors.forename: Elsa C Y
authors.initials: EC
authors.lastname: Yan
mesh_terms: D055162:Biocatalysis; D020134:Catalytic Domain; D002460:Cell Line; D006801:Humans; D006860:Hydrogen Bonding; D006868:Hydrolysis; D007536:Isomerism; D008958:Models, Molecular; D055355:Opsins / Q000378:metabolism; D017354:Point Mutation; D012172:Retinaldehyde / Q000737:chemistry / Q000378:metabolism; D012243:Rhodopsin / Q000235:genetics / Q000502:physiology*; D013056:Spectrophotometry, Ultraviolet; D014785:Vision, Ocular
chemical_list: D055355:Opsins; D012243:Rhodopsin; D012172:Retinaldehyde