title: Protective effects of 2,4-dihydroxybenzophenone against acetaminophen-induced hepatotoxicity in mice.
abstract: OBJECTIVE
  To examine the effects of 2,4-dihydroxybenzophenone (BP-1), a benzophenone derivative used as an ultraviolet light absorbent, on acetaminophen (APAP)-induced hepatotoxicity in C57BL/6J mice.
  
  
  METHODS
  Mice were administered orally with BP-1 at doses of 200, 400 and 800 mg/kg body weight respectively every morning for 4 d before a hepatotoxic dose of APAP (350 mg/kg body weight) was given subcutaneously. Twenty four hours after APAP intoxication, the serum enzyme including serum alaine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH) were measured and liver histopathologic changes were examined.
  
  
  RESULTS
  BP-1 administration dramatically reduced serum ALT, AST and LDH levels. Liver histopathological examination showed that BP-1 administration antagonized APAP-induced liver pathological damage in a dose-dependent manner. Further tests showed that APAP-induced hepatic lipid peroxidation was reduced significantly by BP-1 pretreatment, and glutathione depletion was ameliorated obviously.
  
  
  CONCLUSIONS
  BP-1 can effectively protect C57BL/6J mice from APAP-induced hepatotoxicity, and reduction of oxidative stress might be part of the protection mechanism.
authors.affiliation: Department of Toxicology, School of Public Health, Peking University, Beijing 100191, China.
authors.forename: Yue-Ying
authors.initials: YY
authors.lastname: He
authors.forename: Bao-Xu
authors.initials: BX
authors.lastname: Zhang
authors.forename: Feng-Lan
authors.initials: FL
authors.lastname: Jia
mesh_terms: D000082:Acetaminophen / Q000633:toxicity*; D018712:Analgesics, Non-Narcotic / Q000633:toxicity*; D000818:Animals; D001577:Benzophenones / Q000494:pharmacology / Q000627:therapeutic use*; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy* / Q000517:prevention & control*; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000473:pathology*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018384:Oxidative Stress
chemical_list: D018712:Analgesics, Non-Narcotic; D001577:Benzophenones; D000082:Acetaminophen; D008315:Malondialdehyde; D005978:Glutathione; C121479:2,4-dihydroxybenzophenone