title: Chemicobiological effects of herbicide MCPA-Na on plasma proteins.
abstract: Under physiological conditions, the potential hematological toxicity of herbicide 2-methyl-4-chlorophenoxyacetic acid sodium (MCPA-Na) was discussed by fluorescence probe technology and spectroscopy methods including three-dimensional (3D) fluorescence, UV absorption and circular dichroism (CD) spectra. In vitro, MCPA-Na bound with bovine serum albumin (BSA) and formed new complex at ground state by electrostatic force and hydrogen bond. During the process, non-radiation energy transfer from BSA to MCPA-Na occurred and the distance r between donor and acceptor was obtained based on Förster theory. The binding site was investigated by fluorescence probe method and the results implied MCPA-Na was absorbed on domain II of BSA molecule. The enthalpy change (ΔH(θ)), Gibbs free energy change (ΔG(θ)) and entropy change (ΔS(θ)) were calculated at four different temperatures according to Van't Hoff isobar equation and Gibbs-Helmholtz equation. Negative value of ΔG(θ) indicated the process of binding was a spontaneous and irreversible process, which gave a broad hint that MCPA-Na was likely to be poisonous. CD spectra exhibited significant changes of secondary structures in BSA molecule and three-dimensional fluorescence spectra indicated the tryptophan residue in BSA was placed in a less hydrophobic environment, which presented additional evidence to caution the danger of MCPA-Na residue in food. Meanwhile, the mechanism and geometry of the binding was analyzed at molecular level.
authors.affiliation: College of Chemical and Pharmaceutical Engineering, Jingchu University of Technology, Jingmen, 448000 Hubei, People's Republic of China. h.x.zhang@yeah.net
authors.forename: Hua-xin
authors.initials: HX
authors.lastname: Zhang
authors.forename: Lin
authors.initials: L
authors.lastname: Liu
mesh_terms: D008456:2-Methyl-4-chlorophenoxyacetic Acid / Q000378:metabolism / Q000633:toxicity*; D000818:Animals; D002417:Cattle; D002942:Circular Dichroism; D005456:Fluorescent Dyes; D006540:Herbicides / Q000378:metabolism / Q000633:toxicity*; D015394:Molecular Structure; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature; D013816:Thermodynamics
chemical_list: D005456:Fluorescent Dyes; D006540:Herbicides; D012710:Serum Albumin, Bovine; D008456:2-Methyl-4-chlorophenoxyacetic Acid