title: Maternal-fetal proinflammatory cytokine gene polymorphism and preterm birth.
abstract: Association between maternal-fetal proinflammatory cytokine genotype and preterm birth was studied. Isolated genomic DNA from maternal and cord blood samples of 100 preterm and 101 term labors were used for TNFα (-238G/A, -308G/A), IL-1α (4845G/T), and IL-1β (-511C/T) genotyping. TNFα -238 GA genotype in term neonates was significantly higher than the premature neonates (p<0.05). Maternal-fetal TNFα -238 heterozygosity was associated with term labor (p<0.05). TNFα -308 GA and AA genotypes were associated with term labor (mothers and neonates, respectively; p<0.05 and p<0.001). The incidence of term labor was significantly increased in TNFα -308 GA genotype. If a -308GA carrier has a fetus with GG genotype, the incidence of preterm labor increases (p<0.01). The 4845 T allele was significantly higher in preterm mothers and neonates (p<0.001 and p<0.001). The effect of maternal-fetal genotype for the pregnancy outcome reveals that maternal 4845GG and GT genotypes increase term labor incidence, whereas fetal 4845 TT genotype was a significant independent risk factor for preterm birth (p<0.01). IL-1β -511 TT genotype was significantly higher in preterm neonates. The preterm labor risk was significantly increased in maternal -511 TT genotype and fetal CT genotypes, whereas with maternal -511 CT or TT genotypes or a -511 TT fetus, the incidence of term pregnancy increases (p<0.01).
authors.affiliation: Department of Medical Biology, Baskent University School of Medicine, Ankara, Turkey.
authors.forename: Yaprak
authors.initials: Y
authors.lastname: Yılmaz
authors.forename: Hasibe
authors.initials: H
authors.lastname: Verdi
authors.forename: Ayşe
authors.initials: A
authors.lastname: Taneri
authors.forename: Ayse Canan
authors.initials: AC
authors.lastname: Yazıcı
authors.forename: Ayşe Nur
authors.initials: AN
authors.lastname: Ecevit
authors.forename: Nazmi Mutlu
authors.initials: NM
authors.lastname: Karakaş
authors.forename: Aylin
authors.initials: A
authors.lastname: Tarcan
authors.forename: Ali
authors.initials: A
authors.lastname: Haberal
authors.forename: Namık
authors.initials: N
authors.lastname: Ozbek
authors.forename: Fatma Belgin
authors.initials: FB
authors.lastname: Atac
mesh_terms: D000328:Adult; D004247:DNA / Q000097:blood / Q000235:genetics; D005260:Female; D005312:Fetal Blood / Q000378:metabolism; D005333:Fetus / Q000378:metabolism; D005787:Gene Frequency; D005838:Genotype; D005865:Gestational Age; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature; D018836:Inflammation Mediators / Q000378:metabolism; D053582:Interleukin-1alpha / Q000235:genetics*; D053583:Interleukin-1beta / Q000235:genetics*; D016015:Logistic Models; D008297:Male; D008423:Maternal Age; D007752:Obstetric Labor, Premature / Q000235:genetics; D011110:Polymorphism, Genetic; D011247:Pregnancy; D011256:Pregnancy Outcome / Q000235:genetics; D047928:Premature Birth / Q000235:genetics*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*; D055815:Young Adult
chemical_list: D018836:Inflammation Mediators; D053582:Interleukin-1alpha; D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha; D004247:DNA