title: Characterization of meiotic recombination initiation sites using pulsed-field gel electrophoresis.
abstract: High levels of homologous recombination are induced during meiosis. This meiotic recombination is initiated by programmed formation of DNA double-strand breaks (DSBs) by a conserved meiosis-specific protein, Spo11. Meiotic DSBs are not formed at random along chromosomes but are formed in clusters known as recombination hot spots. To understand the regulation of this initiation step of meiotic recombination, determining the timing and location of meiotic DSBs is essential. In this chapter, we describe a method to detect genome-wide meiotic DSBs by using a combination of pulsed-field gel electrophoresis and Southern blotting.
authors.affiliation: MRC Genome Damage and Stability Centre, University of Sussex, Sussex, UK. s.farmer@sussex.ac.uk
authors.forename: Sarah
authors.initials: S
authors.lastname: Farmer
authors.forename: Wing-Kit
authors.initials: WK
authors.lastname: Leung
authors.forename: Hideo
authors.initials: H
authors.lastname: Tsubouchi
mesh_terms: D015139:Blotting, Southern; D053903:DNA Breaks, Double-Stranded; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D008540:Meiosis / Q000235:genetics*; D011995:Recombination, Genetic / Q000235:genetics*