title: Industrial yeast strain engineered to ferment ethanol from lignocellulosic biomass.
abstract: In this study an industrial Saccharomyces cerevisiae yeast strain capable of fermenting ethanol from pretreated lignocellulosic material was engineered. Genes encoding cellulases (endoglucanase, exoglucanase and β-glucosidase) were integrated into the chromosomal ribosomal DNA and delta regions of a derivative of the K1-V1116 wine yeast strain. The engineered cellulolytic yeast produces ethanol in one step through simultaneous saccharification and fermentation of pretreated biomass without the addition of exogenously produced enzymes. When ethanol fermentation was performed with 10% dry weight of pretreated corn stover, the recombinant strain fermented 63% of the cellulose in 96 h and the ethanol titer reached 2.6% v/v. These results demonstrate that cellulolytic S. cerevisiae strains can be used as a platform for developing an economical advanced biofuel process.
authors.affiliation: Arbor Fuel Inc., 11 Maple Vale Dr., Woodbridge, CT 06525, USA. nkhramtsov@yahoo.com
authors.forename: Nikolai
authors.initials: N
authors.lastname: Khramtsov
authors.forename: Luise
authors.initials: L
authors.lastname: McDade
authors.forename: Alexander
authors.initials: A
authors.lastname: Amerik
authors.forename: Esther
authors.initials: E
authors.lastname: Yu
authors.forename: Kunjan
authors.initials: K
authors.lastname: Divatia
authors.forename: Alexander
authors.initials: A
authors.lastname: Tikhonov
authors.forename: Michael
authors.initials: M
authors.lastname: Minto
authors.forename: Georges
authors.initials: G
authors.lastname: Kabongo-Mubalamate
authors.forename: Zdenek
authors.initials: Z
authors.lastname: Markovic
authors.forename: Marie
authors.initials: M
authors.lastname: Ruiz-Martinez
authors.forename: Steven
authors.initials: S
authors.lastname: Henck
mesh_terms: D001483:Base Sequence; D018533:Biomass; D017931:DNA Primers; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D008031:Lignin / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D015003:Yeasts / Q000378:metabolism*
chemical_list: D017931:DNA Primers; C036909:lignocellulose; D000431:Ethanol; D008031:Lignin