title: Alcohol-induced neuronal death in central extended amygdala and pyriform cortex during the postnatal period of the rat.
abstract: Mothers who consume alcohol during pregnancy may cause a neurotoxic syndrome defined as fetal alcohol spectrum disorder (FASD) in their offspring. This disorder is characterized by reduction in brain size, cognitive deficits and emotional/social disturbances. These alterations are thought to be caused by an alcohol-induced increase in apoptosis during neurodevelopment. Little is known about neuroapoptosis in the central extended amygdala and the pyriform cortex, which are key structures in emotional/social behaviors. The goal of this study was to determine the vulnerability of neuroapoptotic alcohol effects in those areas. Rats were administered alcohol (2.5g/kg s.c. at 0 and 2h) or saline on postnatal day (PND) 7, 15 and 20. The Amino-cupric-silver technique was used to evaluate neurodegeneration and immunohistochemistry to detect activated caspases 3-8 and 9 at 2h, 4, 6, 8, 12 and 24h after drug administration. We measured blood alcohol levels each hour, from 2 to 8h post second administration of alcohol in each of the ages studied. Results showed alcohol induced apoptotic neurodegeneration in the central extended amygdala on PND 7 and 15, and pyriform cortex on PND 7, 15 and 20. These structures showed activation of caspase 3 and 9 but not of caspase 8 suggesting that alcohol-induced apoptosis could occur by the intrinsic pathway. The pharmacokinetic differences between ages did not associate with the neurodegeneration age dependence. In conclusion, these limbic areas are damaged by alcohol, and each one has their own window of vulnerability during the postnatal period. The possible implications in emotional/social features in FASD are discussed.
authors.affiliation: Instituto de Investigación Médica Mercedes y Martín Ferreyra, Friuli, Córdoba, Argentina. vbalaszczuk@psyche.unc.edu.ar
authors.forename: V
authors.initials: V
authors.lastname: Balaszczuk
authors.forename: C
authors.initials: C
authors.lastname: Bender
authors.forename: G L
authors.initials: GL
authors.lastname: Pereno
authors.forename: C A
authors.initials: CA
authors.lastname: Beltramino
mesh_terms: D000428:Alcohol Drinking / Q000009:adverse effects*; D000679:Amygdala / Q000187:drug effects* / Q000473:pathology*; D000818:Animals; D053148:Caspase 3 / Q000378:metabolism; D053181:Caspase 8 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects*; D002492:Central Nervous System Depressants / Q000097:blood / Q000494:pharmacology; D000431:Ethanol / Q000097:blood / Q000494:pharmacology*; D005260:Female; D063647:Fetal Alcohol Spectrum Disorders / Q000473:pathology; D006801:Humans; D008297:Male; D009410:Nerve Degeneration / Q000139:chemically induced / Q000473:pathology; D009833:Olfactory Pathways / Q000187:drug effects* / Q000473:pathology*; D011247:Pregnancy; D011248:Pregnancy Complications / Q000139:chemically induced / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D002492:Central Nervous System Depressants; D000431:Ethanol; D053148:Caspase 3; D053181:Caspase 8; D053453:Caspase 9