title: Controlled comparison of BacT/Alert MB system, manual Myco/F lytic procedure, and isolator 10 system for diagnosis of Mycobacterium tuberculosis Bacteremia.
abstract: We compared the performance of the BacT/Alert MB system, that of the manual Bactec Myco/F Lytic procedure, and that of the Isolator 10 lysis-centrifugation system in the detection of Mycobacterium tuberculosis bacteremia. Mean times to detection were 16.4 days for BacT/Alert MB versus 20.0 days for Myco/F Lytic, 16.5 days for BacT/Alert MB versus 23.8 days for Isolator 10, and 21.1 days for Bactec Myco/F Lytic versus 22.7 days for Isolator 10. There were no significant differences in yields. The mean (range) magnitude of mycobacteremia was 30.0 (0.4, 90.0) CFU/ml and was correlated with the time to positivity in the BacT/Alert MB system (r = -0.4920). M. tuberculosis bacteremia was detected more rapidly in a continuously monitored liquid blood culture system, but the mean time to positivity exceeded 3 weeks.
authors.affiliation: Box 102359, Duke University Medical Center, Durham, NC 27710, USA. john.crump@duke.edu
authors.forename: John A
authors.initials: JA
authors.lastname: Crump
authors.forename: Anne B
authors.initials: AB
authors.lastname: Morrissey
authors.forename: Habib O
authors.initials: HO
authors.lastname: Ramadhani
authors.forename: Boniface N
authors.initials: BN
authors.lastname: Njau
authors.forename: Venance P
authors.initials: VP
authors.lastname: Maro
authors.forename: L Barth
authors.initials: LB
authors.lastname: Reller
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016470:Bacteremia / Q000382:microbiology*; D001431:Bacteriological Techniques / Q000379:methods*; D006801:Humans; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D013997:Time Factors; D014376:Tuberculosis / Q000175:diagnosis* / Q000382:microbiology; D055815:Young Adult