title: Glycine receptors influence radial migration in the embryonic mouse neocortex.
abstract: To investigate whether glycine receptors influence radial migration in the neocortex, we analyzed the effect of glycine and the glycinergic antagonist strychnine, on the distribution of 5-bromo-2'deoxyuridine-labeled neurons in organotypic slice cultures from embryonic mice cortices. Application of glycine impeded radial migration only in the presence of the glycine-transport blockers, ALX-5407 and ALX-1393. This effect was blocked by the specific glycine receptor antagonist strychnine, whereas application of strychnine in the absence of glycine was without effect. We conclude from these observations that an activation of glycine receptors can impede radial migration, but that the glycinergic system is not directly implicated in the regulation of radial migration in organotypic slice cultures.
authors.affiliation: Institute of Physiology and Pathophysiology, University Medical Center of the Johannes Gutenberg University, Mainz, Germany.
authors.forename: Birgit
authors.initials: B
authors.lastname: Nimmervoll
authors.forename: Denise G
authors.initials: DG
authors.lastname: Denter
authors.forename: Irina
authors.initials: I
authors.lastname: Sava
authors.forename: Werner
authors.initials: W
authors.lastname: Kilb
authors.forename: Heiko J
authors.initials: HJ
authors.lastname: Luhmann
mesh_terms: D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D002452:Cell Count; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology*; D004347:Drug Interactions; D004622:Embryo, Mammalian; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D005998:Glycine / Q000494:pharmacology; D018684:Glycine Agents / Q000494:pharmacology; D051379:Mice; D019579:Neocortex / Q000166:cytology*; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D009924:Organ Culture Techniques; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D018009:Receptors, Glycine / Q000378:metabolism*; D012521:Sarcosine / Q000031:analogs & derivatives / Q000494:pharmacology; D013331:Strychnine / Q000494:pharmacology
chemical_list: C510369:(R)-(N-(3-(4'-fluorophenyl)-3-(4'-phenylphenoxy)propyl))sarcosine; D005904:Glial Fibrillary Acidic Protein; D018684:Glycine Agents; D018009:Receptors, Glycine; D010751:Phosphopyruvate Hydratase; D001973:Bromodeoxyuridine; D013331:Strychnine; D005998:Glycine; D012521:Sarcosine