title: Protein assisted fluorescence enhancement of a dansyl containing fluorescent reagent: detection of Hg+ ion in aqueous medium.
abstract: Intramolecular charge transfer (ICT) based fluorescent reagents containing a dansyl fluorophore have been synthesized and characterized. The reagent 1 and its complex, 1+Hg(2+) in sodium acetate buffer (pH 6.7) revealed considerable fluorescence enhancement (switched-on) in the presence of bovine serum albumin (BSA) with 10 ppb detection sensitivity. (1)H NMR spectral analysis suggests complexation between 1 and Hg(2+) ion involving the N,N-dimethylamino and carboxylic functions.
authors.affiliation: Department of Chemistry, Faculty of Science, Banaras Hindu University, Varanasi, 221005, India.
authors.forename: Priyanka
authors.initials: P
authors.lastname: Srivastava
authors.forename: Mohammad
authors.initials: M
authors.lastname: Shahid
authors.forename: Arvind
authors.initials: A
authors.lastname: Misra
mesh_terms: D000818:Animals; D002417:Cattle; D003619:Dansyl Compounds / Q000737:chemistry*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D007477:Ions / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy; D008628:Mercury / Q000032:analysis*; D015394:Molecular Structure; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D003619:Dansyl Compounds; D005456:Fluorescent Dyes; D007477:Ions; D014867:Water; D012710:Serum Albumin, Bovine; D008628:Mercury