title: Evaluation of a commercial microarray as a confirmation test for the presence of extended-spectrum β-lactamases in isolates from the routine clinical setting.
abstract: Since the diagnostic characteristics of the Check-KPC ESBL microarray as a confirmation test on isolates obtained in a routine clinical setting have not been determined, we evaluated the microarray in a random selection of 346 clinical isolates with a positive ESBL screen test (MIC >1 mg/L for cefotaxime or ceftazidime or an ESBL alarm from the Phoenix or Vitek-2 expert system) collected from 31 clinical microbiology laboratories in the Netherlands in 2009. Using sequencing as the reference method the sensitivity of the microarray was 97% (237/245), the specificity 98% (97/99), the positive predictive value 99% (237/239) and the negative predictive value 92% (97/105).
authors.affiliation: SALTRO, Department of Medical Microbiology, University Medical Centre Utrecht, Utrecht, the Netherlands. t.platteel@umcutrecht.nl
authors.forename: T N
authors.initials: TN
authors.lastname: Platteel
authors.forename: J W Cohen
authors.initials: JW
authors.lastname: Stuart
authors.forename: G M
authors.initials: GM
authors.lastname: Voets
authors.forename: J
authors.initials: J
authors.lastname: Scharringa
authors.forename: N
authors.initials: N
authors.lastname: van de Sande
authors.forename: A C
authors.initials: AC
authors.lastname: Fluit
authors.forename: M A
authors.initials: MA
authors.lastname: Leverstein-Van Hall
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods* / Q000592:standards; D004269:DNA, Bacterial / Q000032:analysis; D004755:Enterobacteriaceae / Q000145:classification / Q000201:enzymology* / Q000235:genetics*; D004756:Enterobacteriaceae Infections / Q000382:microbiology; D005798:Genes, Bacterial / Q000235:genetics; D006801:Humans; D046228:Microarray Analysis / Q000379:methods*; D008826:Microbial Sensitivity Tests; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA / Q000379:methods*; D018440:beta-Lactam Resistance / Q000235:genetics; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D001618:beta-Lactamases