title: Evaluation of ethanol production from corncob using Scheffersomyces (Pichia) stipitis CBS 6054 by volumetric scale-up.
abstract: In scale-up, the potential of ethanol production by dilute sulfuric acid pretreatment using corncob was investigated. Pretreatments were performed at 170 °C with various acid concentrations ranging from 0% to 1.656% based on oven dry weight. Following pretreatment, pretreated biomass yield ranged from 59% to 67%. More than 90% of xylan was removed at 0.828% of sulfuric acid. At same pretreatment condition, the highest glucose yield obtained from pretreated biomass by enzymatic hydrolysis was about 76%, based on a glucan content of 37/100 g. In hydrolysate obtained by pretreatment, glucose concentration was low, while xylose concentration was significantly increased above 0.368% of sulfuric acid. At 1.656% of sulfuric acid, xylose and glucose concentration was highest. In subsequent, fermentation with hydrolysate, maximal ethanol yield was attained after 24 h with 0.368% of sulfuric acid. The fermentation efficiency of hydrolysate obtained by enzymatic hydrolysis reached a maximum of 75% at an acid charge of 0.368%.
authors.affiliation: Department of Forest Products and Technology (BK21 Program), College of Agriculture and Life Sciences, Chonnam National University, Gwangju 500-757, South Korea. ljw43376@chonnam.ac.kr
authors.forename: Jae-Won
authors.initials: JW
authors.lastname: Lee
authors.forename: J Y
authors.initials: JY
authors.lastname: Zhu
authors.forename: Danilo
authors.initials: D
authors.lastname: Scordia
authors.forename: Thomas W
authors.initials: TW
authors.lastname: Jeffries
mesh_terms: D056804:Biofuels; D018533:Biomass; D019149:Bioreactors; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000096:biosynthesis; D007218:Industrial Microbiology / Q000379:methods*; D008031:Lignin / Q000378:metabolism*; D010843:Pichia / Q000201:enzymology*; D013464:Sulfuric Acids / Q000737:chemistry*; D014994:Xylose / Q000378:metabolism; D003313:Zea mays / Q000737:chemistry / Q000378:metabolism*
chemical_list: D056804:Biofuels; D013464:Sulfuric Acids; D000431:Ethanol; D008031:Lignin; D014994:Xylose; D005947:Glucose; C033158:sulfuric acid