title: Cytotoxic triterpenoids from Azadirachta indica.
abstract: Two new tirucallane triterpenoids, 24,25-epoxy-3 β-hydroxy-20-oxo-7-tirucallene (1) and 22,23;24,25-diepoxy-3 β-hydroxy-7-tirucallene (2), and a new tetranortriterpenoid, 4 α-hydroperoxy-6- O-acetylnimbandiol (3), along with eight known compounds, were isolated from the branches and leaves of Azadirachta indica. Their structures were elucidated through spectroscopic and chemical methods. The cytotoxic assay showed that the abundant constituent nimbolide (8) had obvious cytotoxic activities against HL-60, SMMC7721, A549, MCF-7, and SW-480 cell lines, with IC₅₀ values of 0.8 ± 0.1, 2.2 ± 0.2, 1.9 ± 1.3, 4.5 ± 1.1, and 2.3 ± 0.1 µM, respectively.
authors.affiliation: Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan, P. R. China.
authors.forename: JinXiong
authors.initials: J
authors.lastname: Chen
authors.forename: JianChao
authors.initials: J
authors.lastname: Chen
authors.forename: Yun
authors.initials: Y
authors.lastname: Sun
authors.forename: YuXin
authors.initials: Y
authors.lastname: Yan
authors.forename: LingMei
authors.initials: L
authors.lastname: Kong
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: MingHua
authors.initials: M
authors.lastname: Qiu
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D031601:Azadirachta / Q000737:chemistry*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D020128:Inhibitory Concentration 50; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: C579591:22,23;24,25-diepoxy-3beta-hydroxy-7-tirucallene; C579588:24,25-epoxy-3beta-hydroxy-20-oxo-7-tirucallene; D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D014315:Triterpenes; C514393:tirucallane