title: [Influence of ionizing radiation, application of iron ions and their chelate complexes on the oxidative status of blood serum of rats].
abstract: Influence of ionizing radiation, ions of iron and their chelate complexes on the oxidative status of blood serum of rats has been investigated. Animals were irradiated by gamma-rays 60Co at a dose of 4 Gy. Ions of iron and iron chelates with nitrilotriacetic acid and citric acid were introduced into animals intra-abdominally at a doze of 10 mg of iron on 1 kg of body weight. The oxidative status of blood serum was determined according to the estimated content of oxidizing peroxide equivalents which oxidize ferrous iron in ferric iron with the subsequent estimation of ferric iron by means of xylenol orange. We also estimated the total content of iron in blood serum using ferrozine as an indicator. The oxidative status was defined 24 and 96 hours after irradiation and 2 hours after introduction of iron ions and their chelates. The research conducted has shown that the concentration of oxidizing peroxide equivalents in serum and the total iron concentration increase 1.47 times and 1.63 times correspondingly 24 hours after irradiation. The increase in the content of oxidizing peroxide equivalents and iron owing to Fenton's reaction can lead to the appearance of OH* radical and raise the level of damage of nuclear and membrane structures in irradiated cells. 2 hours after introduction of iron ions and their chelates, the content of oxidizing peroxide equivalents increased in the blood serum of irradiated and non-irradiated rats, and the maximum effect was observed when introducing ferrous iron and its chelate with citric acid.
authors.forename: N I
authors.initials: NI
authors.lastname: Riabchenko
authors.forename: B P
authors.initials: BP
authors.lastname: Ivannik
authors.forename: V I
authors.initials: VI
authors.lastname: Riabchenko
authors.forename: L A
authors.initials: LA
authors.lastname: Dzikovskaia
mesh_terms: D000818:Animals; D005290:Ferric Compounds / Q000008:administration & dosage / Q000009:adverse effects* / Q000737:chemistry; D005296:Ferrous Compounds / Q000008:administration & dosage / Q000009:adverse effects* / Q000737:chemistry; D005720:Gamma Rays / Q000009:adverse effects*; D007501:Iron / Q000097:blood; D007502:Iron Chelating Agents / Q000008:administration & dosage / Q000009:adverse effects* / Q000737:chemistry; D008297:Male; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000528:radiation effects; D010545:Peroxides / Q000097:blood*; D011829:Radiation Dosage; D011833:Radiation Injuries, Experimental / Q000097:blood*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D005290:Ferric Compounds; D005296:Ferrous Compounds; D007502:Iron Chelating Agents; D010545:Peroxides; D007501:Iron