title: A rapid detection of multidrug-resistant Mycobacterium tuberculosis by a nitrate reductase assay on blood agar.
abstract: The susceptibility of 49 Mycobacterium tuberculosis clinical isolates to isoniazid (INH) and rifampisin (RIF) (28 multi-drug resistant-tuberculosis samples) was determined by a nitrate reductase assay (NRA) on blood agar. Agreement between the NRA and other testing methods was found to be 93.8% for both INH and RIF. The sensitivity, specificity, positive predictive value and negative predictive value for INH were 92.8%, 94.2%, 86.6% and 97%, respectively. The sensitivity, specificity, positive predictive value and negative predictive value for RIF were 90.4%, 96.4%, 95% and 93.1%. In conclusion, we show here that blood agar can be used effectively for the NRA test.
authors.affiliation: Department of Medical Microbiology, Medical School, Ondokuz Mayis University, Samsun, Turkey. cobanay2003@yahoo.com.tr
authors.forename: Ahmet Yilmaz
authors.initials: AY
authors.lastname: Coban
authors.forename: Yeliz Tanriverdi
authors.initials: YT
authors.lastname: Cayci
authors.forename: Aydin
authors.initials: A
authors.lastname: Deveci
authors.forename: Alper
authors.initials: A
authors.lastname: Akgunes
authors.forename: Meltem
authors.initials: M
authors.lastname: Uzun
authors.forename: Belma
authors.initials: B
authors.lastname: Durupinar
mesh_terms: D000362:Agar; D000995:Antitubercular Agents / Q000494:pharmacology*; D024901:Drug Resistance, Multiple, Bacterial / Q000187:drug effects*; D006801:Humans; D007538:Isoniazid / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D009169:Mycobacterium tuberculosis / Q000187:drug effects*; D050901:Nitrate Reductase / Q000378:metabolism; D011237:Predictive Value of Tests; D012293:Rifampin / Q000494:pharmacology*; D012680:Sensitivity and Specificity; D018088:Tuberculosis, Multidrug-Resistant / Q000382:microbiology
chemical_list: D000995:Antitubercular Agents; D000362:Agar; D050901:Nitrate Reductase; D007538:Isoniazid; D012293:Rifampin