title: Use of an automated capillary DNA sequencer to investigate the interaction of cisplatin with telomeric DNA sequences.
abstract: The determination of the sequence selectivity of DNA-damaging agents is very important in elucidating the mechanism of action of anti-tumour drugs. The development of automated capillary DNA sequencers with fluorescent labelling has enabled a more precise method for DNA sequence specificity analysis. In this work we utilized the ABI 3730 capillary sequencer with laser-induced fluorescence to examine the sequence selectivity of cisplatin with purified DNA sequences. The use of this automated machine enabled a higher degree of precision of both position and intensity of cisplatin-DNA adducts than previously possible with manual and automated slab gel procedures. A problem with artefact bands was overcome by ethanol precipitation. It was found that cisplatin strongly formed adducts with telomeric DNA sequences.
authors.affiliation: School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, 2052, Australia.
authors.forename: Moumita
authors.initials: M
authors.lastname: Paul
authors.forename: Vincent
authors.initials: V
authors.lastname: Murray
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry*; D001331:Automation; D001483:Base Sequence; D002945:Cisplatin / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D017931:DNA Primers; D019075:Electrophoresis, Capillary / Q000295:instrumentation*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA / Q000295:instrumentation*; D013050:Spectrometry, Fluorescence; D016615:Telomere
chemical_list: D000970:Antineoplastic Agents; D017931:DNA Primers; D004247:DNA; D002945:Cisplatin