title: ARF triggers cell G1 arrest by a P53 independent ERK pathway.
abstract: In this study, in order to investigate the p53-independent function of p14ARF, we established p14ARF-inducible clones in the p53-deficient HCT cell line using the doxycycline-inducible expression system. A strong cell growth inhibition and G1/S arrest were observed after doxycycline induction in p53-/-HCT cells, and the cells also exhibited an obvious decrease of DNA synthesis. We further examined if the MEK/ERK pathway is involved in the G1 arrest induced by p14ARF in p53-/-HCT cells. The results indicate that ERK1/2 and p21 were activated upon p14ARF induction. Totally, the functional roles of ERK and p21 for ARF in p53-independent tumor suppression were demonstrated.
authors.affiliation: Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, People's Republic of China.
authors.forename: Hansong
authors.initials: H
authors.lastname: Du
authors.forename: Weiqi
authors.initials: W
authors.lastname: Yao
authors.forename: Min
authors.initials: M
authors.lastname: Fang
authors.forename: Dongcheng
authors.initials: D
authors.lastname: Wu
mesh_terms: D001973:Bromodeoxyuridine / Q000494:pharmacology; D002070:Butadienes / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D059585:G1 Phase Cell Cycle Checkpoints / Q000187:drug effects / Q000235:genetics*; D005786:Gene Expression Regulation; D045325:HCT116 Cells; D057809:HEK293 Cells; D006801:Humans; D020935:MAP Kinase Signaling System / Q000187:drug effects / Q000235:genetics*; D009570:Nitriles / Q000494:pharmacology; D010766:Phosphorylation; D025803:Tumor Suppressor Protein p14ARF / Q000235:genetics / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism*; D020741:rho GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D002070:Butadienes; D009570:Nitriles; D025803:Tumor Suppressor Protein p14ARF; D016159:Tumor Suppressor Protein p53; C113580:U 0126; D020741:rho GTP-Binding Proteins; D001973:Bromodeoxyuridine