title: Synthesis of drug/dye-incorporated polymer-protein hybrids.
abstract: We present here a general methodology for significantly increasing the number of dye/drug molecules that can be attached per protein molecule. As a demonstration of this approach, poly(acrylic acid) (PAA)-based near-infrared fluorescence (NIRF) dye- and glucose-incorporated novel copolymers were synthesized, which were further employed for bioconjugation to avidin and bovine serum albumin (BSA). In this method, azide-terminated poly(tert-butyl acrylate) was synthesized via atom transfer radical polymerization (ATRP). Subsequent deprotection was performed to yield poly(acrylic acid) (PAA) possessing a reactive chain-end. A one-pot sequential amidation of the PAA with the amine derivatives of a near-infrared fluorescent dye (ADS832WS) and glucose produced NIRF dye-incorporated water-soluble copolymers. End-group modifications were performed to produce alkyne/biotin-terminated copolymers, which were further employed to generate dye-incorporated polymer-protein hybrids via the biotin-avidin interaction with avidin or by "click" bioconjugation with azide-modified BSA.
authors.forename: Sukanta
authors.initials: S
authors.lastname: Dolai
authors.forename: Wei
authors.initials: W
authors.lastname: Shi
authors.forename: Bikash
authors.initials: B
authors.lastname: Mondal
authors.forename: Krishnaswami
authors.initials: K
authors.lastname: Raja
mesh_terms: D000480:Alkynes / Q000737:chemistry; D000588:Amines / Q000737:chemistry; D000818:Animals; D001360:Avidin / Q000737:chemistry; D001710:Biotin / Q000737:chemistry; D002417:Cattle; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005456:Fluorescent Dyes / Q000737:chemistry*; D005944:Glucosamine / Q000737:chemistry; D011099:Polyglutamic Acid / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry
chemical_list: D000480:Alkynes; D000588:Amines; D005456:Fluorescent Dyes; D011108:Polymers; D011506:Proteins; D001360:Avidin; D011099:Polyglutamic Acid; D012710:Serum Albumin, Bovine; D001710:Biotin; D005944:Glucosamine