title: Detecting weak protein-protein interactions by modified far-western blotting.
abstract: We developed a cross-linking far-western blotting (WB) technique to detect weak protein-protein interactions. In addition, by comparison of the banding patterns resulting from the conventional and cross-linking methods, weak interactions can be identified by monitoring the enhancement of band intensities.
authors.affiliation: Department of Biotechnology, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, Japan.
authors.forename: Yuya
authors.initials: Y
authors.lastname: Sato
authors.forename: Masafumi
authors.initials: M
authors.lastname: Kameya
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Arai
authors.forename: Masaharu
authors.initials: M
authors.lastname: Ishii
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Igarashi
mesh_terms: D026481:Blotting, Far-Western / Q000379:methods*; D006190:Haemophilus / Q000378:metabolism; D006801:Humans; D060066:Protein Interaction Maps; D011506:Proteins / Q000378:metabolism*
chemical_list: D011506:Proteins