title: Low-cost cryo-light microscopy stage fabrication for correlated light/electron microscopy.
abstract: The coupling of cryo-light microscopy (cryo-LM) and cryo-electron microscopy (cryo-EM) poses a number of advantages for understanding cellular dynamics and ultrastructure. First, cells can be imaged in a near native environment for both techniques. Second, due to the vitrification process, samples are preserved by rapid physical immobilization rather than slow chemical fixation. Third, imaging the same sample with both cryo-LM and cryo-EM provides correlation of data from a single cell, rather than a comparison of "representative samples". While these benefits are well known from prior studies, the widespread use of correlative cryo-LM and cryo-EM remains limited due to the expense and complexity of buying or building a suitable cryogenic light microscopy stage. Here we demonstrate the assembly, and use of an inexpensive cryogenic stage that can be fabricated in any lab for less than $40 with parts found at local hardware and grocery stores. This cryo-LM stage is designed for use with reflected light microscopes that are fitted with long working distance air objectives. For correlative cryo-LM and cryo-EM studies, we adapt the use of carbon coated standard 3-mm cryo-EM grids as specimen supports. After adsorbing the sample to the grid, previously established protocols for vitrifying the sample and transferring/handling the grid are followed to permit multi-technique imaging. As a result, this setup allows any laboratory with a reflected light microscope to have access to direct correlative imaging of frozen hydrated samples.
authors.affiliation: Department of Molecular and Cellular Biology, University of California Davis, USA.
authors.forename: David B
authors.initials: DB
authors.lastname: Carlson
authors.forename: James E
authors.initials: JE
authors.lastname: Evans
mesh_terms: D020285:Cryoelectron Microscopy / Q000191:economics / Q000295:instrumentation* / Q000379:methods*; D008027:Light; D008853:Microscopy / Q000191:economics / Q000295:instrumentation* / Q000379:methods*; D015003:Yeasts / Q000166:cytology