title: Prevalence and genetic relatedness of methicillin-susceptible Staphylococcus aureus isolates detected by the Xpert MRSA nasal assay.
abstract: Methicillin-susceptible Staphylococcus aureus (MSSA) isolates lacking mecA yet testing positive on the Xpert MRSA assay were recovered from culture for 7.7% of 248 Xpert-positive nasal samples. These "false-positive" Xpert results may be attributed to staphylococcal cassette chromosome (SCC) elements without the mecA gene. Pulsed-field gel electrophoresis (PFGE) analysis revealed a diverse population of MSSA strains.
authors.affiliation: Department of Clinical Pathology, Cleveland Clinic, 9500 Euclid Avenue, L40, Cleveland, OH 44195, USA.
authors.forename: Sophie S
authors.initials: SS
authors.lastname: Arbefeville
authors.forename: Kunyan
authors.initials: K
authors.lastname: Zhang
authors.forename: Jennifer S
authors.initials: JS
authors.lastname: Kroeger
authors.forename: Wanita J
authors.initials: WJ
authors.lastname: Howard
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Diekema
authors.forename: Sandra S
authors.initials: SS
authors.lastname: Richter
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics; D001431:Bacteriological Techniques / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics; D003951:Diagnostic Errors; D006801:Humans; D008712:Methicillin / Q000494:pharmacology*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D046915:Penicillin-Binding Proteins; D013203:Staphylococcal Infections / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D046915:Penicillin-Binding Proteins; C487789:mecA protein, Staphylococcus aureus; D008712:Methicillin