title: LvBMP-2 gene-modified BMSCs combined with calcium phosphate cement scaffolds for the repair of calvarial defects in rats.
abstract: The study aims to evaluate the effect of bone marrow stromal cells (BMSCs) expressing bone morphogenic protein-2 (BMP-2) mediated by lentiviral (Lv) gene transduction combined with calcium phosphate cement (CPC) scaffolds for the repair of critical size calvarial defects in rats. BMSCs derived from Fisher 344 rats were transduced with LvBMP-2 or lentivirus encoding enhanced green fluorescent protein (LvEGFP) in vitro. Obvious osteogenic differentiation of BMSCs in the LvBMP-2 group was demonstrated by alkaline phosphatase staining and alizarin red staining. Enzyme-linked immunosorbent assay results show that LvBMP-2 gene expression in vitro can last for at least 8 weeks. Gene-transduced or untransduced BMSCs were seeded onto CPC scaffolds to repair rat calvarial defects with a diameter of 5 mm. Scanning electron microscope analysis indicated that porous CPC scaffolds facilitated initial adhesion and spreading of BMSCs onto its surface. Calvarial defects were successfully repaired with LvBMP-2-transduced BMSCs/CPC constructs 8 weeks postoperatively. The percentage of new bone formation in the LvBMP-2 group was significantly higher than in other control groups. Lentiviral mediated BMP-2 gene therapy together with CPC scaffolds can be used successfully in calvarial repair and bone regeneration.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200011, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhu
authors.forename: Qing
authors.initials: Q
authors.lastname: Chang
authors.forename: Duohong
authors.initials: D
authors.lastname: Zou
authors.forename: Wenjie
authors.initials: W
authors.lastname: Zhang
authors.forename: Shaoyi
authors.initials: S
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Zhao
authors.forename: Wenwen
authors.initials: W
authors.lastname: Yu
authors.forename: Xiuli
authors.initials: X
authors.lastname: Zhang
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Zhang
authors.forename: Xinquan
authors.initials: X
authors.lastname: Jiang
mesh_terms: D000818:Animals; D001843:Bone Cements / Q000737:chemistry*; D001854:Bone Marrow Cells / Q000378:metabolism*; D055396:Bone Morphogenetic Protein 2 / Q000235:genetics*; D002130:Calcium Phosphates / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D008855:Microscopy, Electron, Scanning; D051381:Rats; D011916:Rats, Inbred F344; D017154:Stromal Cells / Q000378:metabolism*
chemical_list: D001843:Bone Cements; D055396:Bone Morphogenetic Protein 2; D002130:Calcium Phosphates; C020243:calcium phosphate