title: Determination of dipyrone in pharmaceutical preparations based on the chemiluminescent reaction of the quinolinic hydrazide-H2O2-vanadium(IV) system and flow-injection analysis.
abstract: A rapid, economic and sensitive chemiluminescent method involving flow-injection analysis was developed for the determination of dipyrone in pharmaceutical preparations. The method is based on the chemiluminescent reaction between quinolinic hydrazide and hydrogen peroxide in a strongly alkaline medium, in which vanadium(IV) acts as a catalyst. Principal chemical and physical variables involved in the flow-injection system were optimized using a modified simplex method. The variations in the quantum yield observed when dipyrone was present in the reaction medium were used to determine the concentration of this compound. The proposed method requires no preconcentration steps and reliably quantifies dipyrone over the linear range 1-50 µg/mL. In addition, a sample throughput of 85 samples/h is possible.
authors.affiliation: Departamento de Ciencias Analíticas, Facultad de Ciencias, Universidad Nacional de Educación a Distancia, Madrid, Spain.
authors.forename: Juan A
authors.initials: JA
authors.lastname: Pradana Pérez
authors.forename: Jesús S
authors.initials: JS
authors.lastname: Durand Alegría
authors.forename: Pilar Fernández
authors.initials: PF
authors.lastname: Hernando
authors.forename: Adolfo Narros
authors.initials: AN
authors.lastname: Sierra
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal; D004177:Dipyrone / Q000032:analysis*; D017022:Flow Injection Analysis / Q000379:methods*; D006834:Hydrazines / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000737:chemistry; D008163:Luminescent Measurements / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000032:analysis*; D014639:Vanadium / Q000737:chemistry
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D006834:Hydrazines; D004364:Pharmaceutical Preparations; D014639:Vanadium; D004177:Dipyrone; D006861:Hydrogen Peroxide