title: The correlation of hepatitis B virus pre-S mutation with mitochondrial D-loop mutations and common deletions in hepatocellular carcinoma.
abstract: OBJECTIVE
  We investigated the correlation of HBV pre-S mutation with the sites and frequencies of mtDNA mutations in HCC patients.
  
  
  METHODS
  Twenty-seven HBV-related HCC patients and 8 control patients were included. HBV DNA was extracted from sera and the HBV S coding region was analyzed. Direct sequencing of the mtDNA D-loop was performed in paired HCC and adjacent non-neoplastic liver tissues. The common 4977 bp deletion of miDNA was examined by PCR.
  
  
  RESULTS
  Study subjects were categorized into three groups: the pre-S mutant HBV-infected HCC patients (group 1), wild-type HBV-infected HCC patients (group 2) and HBV non-infected patients (group 3). The frequency of mtDNA D-loop mutations in non-neoplastic tissue was higher in group 1/2 than in group 3; however, there was no significant difference between group 1 and 2. The frequency of mtDNA D-loop mutations showed no significant difference between HCC and nonneoplastic tissues. The prevalence of the common 4977 bp deletion was lower in HCC compared to non-neoplastic tissues, however, there was no difference according to the pre-S mutation.
  
  
  CONCLUSIONS
  The present study does not support a pathophysiological role for the HBV pre-S mutation, related to mtDNA D-loop mutation or alteration of the common 4977 bp deletion, in hepatocarcinogenesis.
authors.affiliation: Department of Medicine and Digestive Disease Research Center, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea.
authors.forename: Geum-Youn
authors.initials: GY
authors.lastname: Gwak
authors.forename: Dong Ho
authors.initials: DH
authors.lastname: Lee
authors.forename: Tae Gun
authors.initials: TG
authors.lastname: Moon
authors.forename: Moon Suk
authors.initials: MS
authors.lastname: Choi
authors.forename: Joon Hyeok
authors.initials: JH
authors.lastname: Lee
authors.forename: Kwang Cheol
authors.initials: KC
authors.lastname: Koh
authors.forename: Seung Woon
authors.initials: SW
authors.lastname: Paik
authors.forename: Jae-Won
authors.initials: JW
authors.lastname: Joh
authors.forename: Byung Chul
authors.initials: BC
authors.lastname: Yoo
mesh_terms: D000328:Adult; D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000209:etiology* / Q000235:genetics / Q000821:virology; D004272:DNA, Mitochondrial / Q000235:genetics*; D005260:Female; D017353:Gene Deletion; D006514:Hepatitis B Surface Antigens / Q000235:genetics*; D006801:Humans; D008113:Liver Neoplasms / Q000209:etiology* / Q000235:genetics / Q000821:virology; D008297:Male; D008875:Middle Aged; D009154:Mutation; D011498:Protein Precursors / Q000235:genetics*
chemical_list: D004272:DNA, Mitochondrial; D006514:Hepatitis B Surface Antigens; D011498:Protein Precursors; C050806:presurface protein 1, hepatitis B surface antigen; C050807:presurface protein 2, hepatitis B surface antigen