title: Diblock glycopolymers promote colloidal stability of polyplexes and effective pDNA and siRNA delivery under physiological salt and serum conditions.
abstract: A series of glycopolymers composed of 2-deoxy-2-methacrylamido glucopyranose (MAG) and the primary amine-containing N-(2-aminoethyl) methacrylamide (AEMA) were synthesized via aqueous reversible addition-fragmentation chain transfer (RAFT) polymerization. The colloidal stability of the polyplexes formed with three diblock glycopolymers and pDNA was assessed using dynamic light scattering, and the polyplexes were found to be stable against aggregation in the presence of salt and serum over the 4 h time period studied. Delivery experiments were performed in vitro to examine the cellular uptake, transfection efficiency, and cytotoxicity of the glycopolymer/pDNA polyplexes in cultured HeLa cells and the diblock copolymer with the shortest AEMA block was found to be the most effective. Additionally, the ability of the diblock glycopolymers to deliver siRNA to U-87 (glioblastoma) cells was screened, and the diblock copolymer with the longest AEMA block was found to have gene knockdown efficacy similar to Lipofectamine 2000.
authors.affiliation: Macromolecules and Interfaces Institute, Department of Chemistry, Virginia Tech, Blacksburg, Virginia 24061, United States.
authors.forename: Adam E
authors.initials: AE
authors.lastname: Smith
authors.forename: Antons
authors.initials: A
authors.lastname: Sizovs
authors.forename: Giovanna
authors.initials: G
authors.lastname: Grandinetti
authors.forename: Lian
authors.initials: L
authors.lastname: Xue
authors.forename: Theresa M
authors.initials: TM
authors.lastname: Reineke
mesh_terms: D001769:Blood; D003102:Colloids; D004247:DNA / Q000008:administration & dosage*; D006367:HeLa Cells; D006801:Humans; D046529:Microscopy, Electron, Transmission; D010957:Plasmids; D011108:Polymers / Q000737:chemistry*; D034741:RNA, Small Interfering / Q000008:administration & dosage*; D012492:Salts
chemical_list: D003102:Colloids; D011108:Polymers; D034741:RNA, Small Interfering; D012492:Salts; D004247:DNA