title: Expression and clinical significance of cathepsin B and stefin A in laryngeal cancer.
abstract: The aim of this study was to investigate the roles of the cathepsin B cysteine protease and its endogenous inhibitor stefin A in laryngeal cancer. Immunohistochemistry was employed to detect the expression of cathepsin B and stefin A in 84 patients with laryngeal cancer, respectively. The protein expression of stefin A was negatively associated with lymphatic metastasis, recurrence of laryngeal cancer and the survival rate, which was not observed with cathepsin B protein expression. Both down-regulation of cathepsin B and up-regulation of stefin A in vitro significantly inhibited the migration, invasion and proliferation of laryngeal cancer cells, respectively. Our results strongly suggest that stefin A may be a potential predictor of laryngeal cancer and may be used in the molecular diagnosis and gene therapy of laryngeal cancer. Cathepsin B may be used as a promising therapeutic target in the treatment of laryngeal cancer.
authors.affiliation: Department of Respiratory Diseases, Chinese PLA General Hospital, Beijing 100853, PR China.
authors.forename: Chunsun
authors.initials: C
authors.lastname: Li
authors.forename: Liwei
authors.initials: L
authors.lastname: Chen
authors.forename: Jialing
authors.initials: J
authors.lastname: Wang
authors.forename: Liyong
authors.initials: L
authors.lastname: Zhang
authors.forename: Pingzhang
authors.initials: P
authors.lastname: Tang
authors.forename: Suoqiang
authors.initials: S
authors.lastname: Zhai
authors.forename: Weiwei
authors.initials: W
authors.lastname: Guo
authors.forename: Ning
authors.initials: N
authors.lastname: Yu
authors.forename: Lidong
authors.initials: L
authors.lastname: Zhao
authors.forename: Mingbo
authors.initials: M
authors.lastname: Liu
authors.forename: Shiming
authors.initials: S
authors.lastname: Yang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D043402:Cathepsin A / Q000096:biosynthesis* / Q000235:genetics; D002401:Cathepsin B / Q000096:biosynthesis* / Q000235:genetics; D055312:Cystatin A / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007822:Laryngeal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D014162:Transfection
chemical_list: D055312:Cystatin A; C026407:CSTA protein, human; C468629:CTSA protein, human; D043402:Cathepsin A; C533912:CTSB protein, human; D002401:Cathepsin B