title: Enzyme-linked immunosorbent assays for α-synuclein with species and multimeric state specificities.
abstract: Abnormal intracellular deposition of aggregated α-synuclein is the characteristic feature of a number of neurological disorders, including Parkinson's disease (PD). Although α-synuclein is typically known as a cytosolic protein, a small amount is secreted by exocytosis in both monomeric and aggregated forms. The extracellular forms of α-synuclein in human body fluids, such as cerebrospinal fluid (CSF) and blood plasma, might be a diagnostic target for PD and related diseases. Here, we characterized a new set of monoclonal antibodies against α-synuclein, and using different combinations of antibodies, we established ELISA systems to specifically detect human α-synuclein, mouse and human α-synuclein together, and multimeric forms of α-synuclein in biological samples. By employing the Tyramide signal amplification method, the sensitivity of the assay was significantly improved to detect a concentration as low as ∼12.5 pg/ml. These assays might be useful tools for quantitative analysis of α-synuclein in various forms and with high sensitivity in diverse biological samples.
authors.affiliation: Department of Anatomy, School of Medicine, Konkuk University, Seoul 143-701, South Korea.
authors.forename: He-Jin
authors.initials: HJ
authors.lastname: Lee
authors.forename: Eun-Jin
authors.initials: EJ
authors.lastname: Bae
authors.forename: Ara
authors.initials: A
authors.lastname: Jang
authors.forename: Dong-Hwan
authors.initials: DH
authors.lastname: Ho
authors.forename: Eun-Duk
authors.initials: ED
authors.lastname: Cho
authors.forename: Ji-Eun
authors.initials: JE
authors.lastname: Suk
authors.forename: Yeo-Min
authors.initials: YM
authors.lastname: Yun
authors.forename: Seung-Jae
authors.initials: SJ
authors.lastname: Lee
mesh_terms: D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D000918:Antibody Specificity; D019556:COS Cells; D002522:Chlorocebus aethiops; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D000939:Epitopes / Q000276:immunology*; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010300:Parkinson Disease / Q000097:blood / Q000134:cerebrospinal fluid / Q000276:immunology; D055503:Protein Multimerization / Q000276:immunology; D055815:Young Adult; D051844:alpha-Synuclein / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D051844:alpha-Synuclein