title: New diagnostic microarray (Check-KPC ESBL) for detection and identification of extended-spectrum beta-lactamases in highly resistant Enterobacteriaceae.
abstract: The performance of a microarray for the detection of extended-spectrum beta-lactamases was determined on a collection of 638 highly resistant members of the family Enterobacteriaceae collected from patients in 18 hospitals in The Netherlands. The microarray had a significantly higher specificity than the phenotypic assays. It also detects carbapenemases and characterizes the resistance genes, providing epidemiological insight.
authors.affiliation: Laboratory of Microbiology and Infection Control, Amphia Hospital, P.O. Box 90158, 4800 RK Breda, The Netherlands.
authors.forename: Ina
authors.initials: I
authors.lastname: Willemsen
authors.forename: Ilse
authors.initials: I
authors.lastname: Overdevest
authors.forename: Nashwan
authors.initials: N
authors.lastname: Al Naiemi
authors.forename: Martine
authors.initials: M
authors.lastname: Rijnsburger
authors.forename: Paul
authors.initials: P
authors.lastname: Savelkoul
authors.forename: Christina
authors.initials: C
authors.lastname: Vandenbroucke-Grauls
authors.forename: Jan
authors.initials: J
authors.lastname: Kluytmans
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D024881:Drug Resistance, Bacterial; D004755:Enterobacteriaceae / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000382:microbiology*; D005798:Genes, Bacterial; D005838:Genotype; D006801:Humans; D046228:Microarray Analysis / Q000379:methods*; D009426:Netherlands; D012680:Sensitivity and Specificity; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D001618:beta-Lactamases