title: DNA analysis based on the local structural disruption to the duplexes carrying a luminous lanthanide complex.
abstract: The two DNA conjugates (split probes) carrying a metal chelator form an integrated luminous lanthanide (Ln(3+): Tb(3+) or Eu(3+)) complex on the complementary template DNA (target). The luminous property of this Ln(3+) complex has been used for DNA assay. The intensity of the luminescence was affected by the local structural disruption caused by one-base mispairing around the complex. Among the mispairings systematically introduced around the Ln(3+) center, vicinal mispairings to the center decreased the emission intensity more. This would be a novel nucleobase-discriminating principle, in which the split probes bind the target tightly, yet still retain sequence selectivity.
authors.affiliation: Department of Applied Chemistry and Biochemistry, Graduate School of Science and Technology, Kumamoto University, 2-39-1 Kurokami, Kumamoto 860-8555, Japan. toshi@chem.kumamoto-u.ac.jp
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Ihara
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Kitamura
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Tsujimura
authors.forename: Akinori
authors.initials: A
authors.lastname: Jyo
mesh_terms: D002614:Chelating Agents / Q000737:chemistry*; D004247:DNA / Q000032:analysis*; D028581:Lanthanoid Series Elements / Q000737:chemistry*; D049449:Luminescence; D008163:Luminescent Measurements; D008958:Models, Molecular; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000737:chemistry*; D013696:Temperature
chemical_list: D002614:Chelating Agents; D028581:Lanthanoid Series Elements; D009942:Organometallic Compounds; D004247:DNA