title: Identification and transcription profiling of trypsin in Aedes taeniorhynchus (Diptera: Culicidae): developmental regulation, blood feeding, and permethrin exposure.
abstract: The cDNA of a trypsin gene from Aedes (Ochlerotatus) taeniorhynchus (Weidemann) was cloned and sequenced. The full-length mRNA sequence (890 bp) for trypsin from Ae. taeniorhynchus (AetTryp1) was obtained, which encodes an open reading frame of 765 bp (i.e., 255 amino acids). To detect whether AetTryp is developmentally regulated, a quantitative real-time polymerase chain reaction was used to examine AetTrypl mRNA expression levels in different developmental stages of Ae. taeniorhynchus. AetTryp1 was expressed at low levels in egg, larval, and pupal stages, but was differentially expressed in adult Ae. taeniorhynchus, with highest levels found in 5-d-old female adults when compared with teneral adults. In addition, AetTryp1 mRNA expression differed between sexes, with expression levels much lower in males. However, in both males and females, there was a significant increase in AetTryp1 transcription levels as age increased and peaked in 5-d-old adults. AetTrypl expressed in 5-d-old female Ae. taeniorhynchus significantly increased after 30 min postblood feeding compared with the control. The AetTryp1 mRNA expression in 5-d-old female Ae. taeniorhynchus was affected by different concentrations of permethrin.
authors.affiliation: Biological Control of Pests Research Unit, Mid-Southern Area-United States Department of Agriculture-Agricultural Research Service, 59 Lee Road, Stoneville, MS 38776, USA. liming.zhao@ars.usda.gov
authors.forename: Liming
authors.initials: L
authors.lastname: Zhao
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.forename: James J
authors.initials: JJ
authors.lastname: Becnel
authors.forename: Daniel L
authors.initials: DL
authors.lastname: Kline
authors.forename: Gary G
authors.initials: GG
authors.lastname: Clark
authors.forename: Kenneth J
authors.initials: KJ
authors.lastname: Linthicum
mesh_terms: D000818:Animals; D005247:Feeding Behavior / Q000187:drug effects; D005260:Female; D018507:Gene Expression Regulation, Developmental; D019476:Insect Proteins / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D008018:Life Cycle Stages / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D008297:Male; D008969:Molecular Sequence Data; D044969:Ochlerotatus / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D026023:Permethrin / Q000494:pharmacology*; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D014357:Trypsin / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism
chemical_list: D019476:Insect Proteins; D012333:RNA, Messenger; D026023:Permethrin; D014357:Trypsin