title: High-content screening in infectious diseases.
abstract: The last decade has seen the development of automated microscopy and its adaptation for various areas of research, particularly infectious disease. Most of the high-content screening (HCS) platforms now integrate all of the following necessary steps: automated pipettes for assay miniaturization in 384-well plates, automated image acquisition and data storage and analysis. HCS was initially associated with RNA interference genetic screens for identifying host factors involved in host-pathogen interactions. More recently, both in academia and in industry, HCS has been adapted for drug discovery purposes. High-content analysis enables intracellular tracking of viral particles to profile the antiviral mechanisms of each compound. Adaptation to high-throughput screening in bacteriology and parasitology has already led to the discovery of new types of host-specific inhibitors that differ from those inhibitors that act directly on microbes.
authors.affiliation: Biology of Intracellular Pathogens Inserm Avenir Team, Republic of Korea. priscile.brodin@inserm.fr
authors.forename: Priscille
authors.initials: P
authors.lastname: Brodin
authors.forename: Thierry
authors.initials: T
authors.lastname: Christophe
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000977:Antiparasitic Agents / Q000494:pharmacology*; D000998:Antiviral Agents / Q000494:pharmacology*; D001681:Biological Assay; D003141:Communicable Diseases / Q000188:drug therapy*; D055808:Drug Discovery; D005820:Genetic Testing / Q000379:methods*; D057166:High-Throughput Screening Assays / Q000379:methods*; D054884:Host-Pathogen Interactions; D006801:Humans; D008904:Miniaturization; D034622:RNA Interference; D013329:Structure-Activity Relationship
chemical_list: D000900:Anti-Bacterial Agents; D000977:Antiparasitic Agents; D000998:Antiviral Agents