title: Phosphonate-titanium dioxide assemblies: platform for multimodal diagnostic-therapeutic nanoprobes.
abstract: Multimodal imaging-therapeutic nanoprobe TiO(2)@RhdGd was prepared and successfully used for in vitro and in vivo cell tracking as well as for killing of cancer cells in vitro. TiO(2) nanoparticles were used as a core for phosphonic acid modified functionalities, responsible for contrast in MRI and optical imaging. The probe shows high (1)H relaxivity and relaxivity density values. Presence of fluorescent dye allows for visualization by means of fluorescence microscopy. The applicability of the probe was studied, using mesenchymal stem cells, cancer HeLa cells, and T-lymphocytes. The probe did not exhibit toxicity in any of these systems. Labeled cells were successfully visualized in vitro by means of fluorescence microscopy and MRI. Furthermore, it was shown that the probe TiO(2)@RhdGd can be changed into a cancer cell killer upon UV light irradiation. The above stated results represent a valuable proof of a principle showing applicability of the probe design for diagnosis and therapy.
authors.affiliation: Department of Inorganic Chemistry, Faculty of Science, Charles University in Prague, Hlavova 2030, 128 40 Prague 2, Czech Republic.
authors.forename: Ivan
authors.initials: I
authors.lastname: Řehoř
authors.forename: Vanda
authors.initials: V
authors.lastname: Vilímová
authors.forename: Pavla
authors.initials: P
authors.lastname: Jendelová
authors.forename: Vojtěch
authors.initials: V
authors.lastname: Kubíček
authors.forename: Daniel
authors.initials: D
authors.lastname: Jirák
authors.forename: Vít
authors.initials: V
authors.lastname: Herynek
authors.forename: Miroslava
authors.initials: M
authors.lastname: Kapcalová
authors.forename: Jan
authors.initials: J
authors.lastname: Kotek
authors.forename: Jan
authors.initials: J
authors.lastname: Černý
authors.forename: Petr
authors.initials: P
authors.lastname: Hermann
authors.forename: Ivan
authors.initials: I
authors.lastname: Lukeš
mesh_terms: D000345:Affinity Labels / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D005682:Gadolinium; D006367:HeLa Cells; D006801:Humans; D008279:Magnetic Resonance Imaging; D059630:Mesenchymal Stem Cells / Q000378:metabolism; D051379:Mice; D008856:Microscopy, Fluorescence; D053758:Nanoparticles; D063065:Organophosphonates / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D013329:Structure-Activity Relationship; D013601:T-Lymphocytes / Q000378:metabolism; D014025:Titanium / Q000737:chemistry* / Q000494:pharmacology; D014466:Ultraviolet Rays
chemical_list: D000345:Affinity Labels; D000970:Antineoplastic Agents; D056831:Coordination Complexes; D005456:Fluorescent Dyes; D063065:Organophosphonates; C009495:titanium dioxide; D005682:Gadolinium; D014025:Titanium