title: Sequence-specific binding to telomeric DNA is not a conserved property of the Cdc13 DNA binding domain.
abstract: In the budding yeast Saccharomyces cerevisiae, chromosome end protection is provided by a heterotrimeric complex composed of Cdc13 in association with the RPA-like proteins Stn1 and Ten1. We report here that the high affinity and specificity of the S. cerevisiae Cdc13 DNA binding domain for single-stranded telomeric DNA are not widely shared by other fungal Cdc13 proteins, suggesting that restriction of this complex to telomeres may be limited to the Saccharomyces clade. We propose that the evolutionarily conserved task of Stn1 and Ten1 (and their associated large subunit) is a genome-wide role in DNA replication rather than a telomere-dedicated activity.
authors.affiliation: Salk Institute for Biological Studies, La Jolla, California 92037, USA.
authors.forename: Edward K
authors.initials: EK
authors.lastname: Mandell
authors.forename: Amy D
authors.initials: AD
authors.lastname: Gelinas
authors.forename: Deborah S
authors.initials: DS
authors.lastname: Wuttke
authors.forename: Victoria
authors.initials: V
authors.lastname: Lundblad
mesh_terms: D001483:Base Sequence; D002176:Candida albicans / Q000378:metabolism; D004271:DNA, Fungal / Q000378:metabolism*; D007700:Kinetics; D011485:Protein Binding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000378:metabolism*; D016615:Telomere / Q000378:metabolism*; D034501:Telomere-Binding Proteins / Q000737:chemistry* / Q000378:metabolism*
chemical_list: C452219:Cdc13 protein, S cerevisiae; D004271:DNA, Fungal; D029701:Saccharomyces cerevisiae Proteins; D034501:Telomere-Binding Proteins