title: The production of extracellular proteins is regulated by ribonuclease III via two different pathways in Staphylococcus aureus.
abstract: Staphylococcus aureus ribonuclease III belongs to the enzyme family known to degrade double-stranded RNAs. It has previously been reported that RNase III cannot influence cell growth but regulates virulence gene expression in S. aureus. Here we constructed an RNase III inactivation mutant (Δrnc) from S. aureus 8325-4. It was found that the extracellular proteins of Δrnc were decreased. Furthermore, we explored how RNase III regulated the production of the extracellular proteins in S. aureus. We found during the lag phase of the bacterial growth cycle RNase III could influence the extracellular protein secretion via regulating the expression of secY2, one component of accessory secretory (sec) pathway. After S. aureus cells grew to exponential phase, RNase III can regulate the expression of extracellular proteins by affecting the level of RNAIII. Further investigation showed that the mRNA stability of secY2 and RNAIII was affected by RNase III. Our results suggest that RNase III could regulate the pathogenicity of S. aureus by influencing the level of extracellular proteins via two different ways respectively at different growth phases.
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing, People's Republic of China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.forename: Jie
authors.initials: J
authors.lastname: Dong
authors.forename: Na
authors.initials: N
authors.lastname: Wu
authors.forename: Yaping
authors.initials: Y
authors.lastname: Gao
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Chunhua
authors.initials: C
authors.lastname: Mu
authors.forename: Ningsheng
authors.initials: N
authors.lastname: Shao
authors.forename: Ming
authors.initials: M
authors.lastname: Fan
authors.forename: Guang
authors.initials: G
authors.lastname: Yang
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism*; D015152:Blotting, Northern; D015153:Blotting, Western; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics / Q000502:physiology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012330:RNA, Double-Stranded / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D043244:Ribonuclease III / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology; D013211:Staphylococcus aureus / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D012330:RNA, Double-Stranded; D043244:Ribonuclease III