title: Identification and characterization of the Arabidopsis FG-repeat nucleoporin Nup62.
abstract: Ran is a multifunctional small GTPase that is involved in nucleocytoplasmic transport, mitotic spindle assembly, and nuclear envelope reformation. Nuclear transport factor 2 (NTF2) facilitates nuclear import of Ran. It binds FxFG repeat-containing domains of the nucleoporins Nup62 (vertebrate) and Nsp1p (yeast). Here, we have identified Arabidopsis Nup62 through its sequence similarity to mammalian Nup62 and yeast Nsp1p. A GFP¬AtNup62 fusion protein is associated with the nuclear envelope in transgenic Arabidopsis plants and interacts in planta with AtNTF2a, one of the two Arabidopsis NTF2 homologs. Overexpression-based co-suppression of AtNup62 leads to severely dwarfed, early-flowering plants, suggesting an important function for Nup62 in plants.
authors.affiliation: Department of Plant Cellular and Molecular Biology Plant Bio Technology Center, The Ohio State University, Columbus, OH, USA.
authors.forename: Qiao
authors.initials: Q
authors.lastname: Zhao
authors.forename: Iris
authors.initials: I
authors.lastname: Meier
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D047468:Immunoprecipitation; D008969:Molecular Sequence Data; D028861:Nuclear Pore Complex Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D029543:Nucleocytoplasmic Transport Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D028861:Nuclear Pore Complex Proteins; D029543:Nucleocytoplasmic Transport Proteins