title: Qualitative and quantitative resistances to leaf rust finely mapped within two nucleotide-binding site leucine-rich repeat (NBS-LRR)-rich genomic regions of chromosome 19 in poplar.
abstract: • R(US) is a major dominant gene controlling quantitative resistance, inherited from Populus trichocarpa, whereas R(1) is a gene governing qualitative resistance, inherited from P. deltoides. • Here, we report a reiterative process of concomitant fine-scale genetic and physical mapping guided by the P. trichocarpa genome sequence. The high-resolution linkage maps were developed using a P. deltoides × P. trichocarpa progeny of 1415 individuals. R(US) and R(1) were mapped in a peritelomeric region of chromosome 19. Markers closely linked to R(US) were used to screen a bacterial artificial chromosome (BAC) library constructed from the P. trichocarpa parent, heterozygous at the locus R(US) . • Two local physical maps were developed, one encompassing the R(US) allele and the other spanning r(US) . The alignment of the two haplophysical maps showed structural differences between haplotypes. The genetic and physical maps were anchored to the genome sequence, revealing genome sequence misassembly. Finally, the R(US) locus was localized within a 0.8-cM interval, whereas R(1) was localized upstream of R(US) within a 1.1-cM interval. • The alignment of the genetic and physical maps with the local reorder of the chromosome 19 sequence indicated that R(US) and R(1) belonged to a genomic region rich in nucleotide-binding site leucine-rich repeat (NBS-LRR) and serine threonine kinase (STK) genes.
authors.affiliation: INRA, UMR1165, UMR INRA/Université de Evry: Unité de Recherche en Génomique Végétale, Centre de Recherche de Versailles-Grignon, Evry Cedex, 91057, France.
authors.forename: Aloïs
authors.initials: A
authors.lastname: Bresson
authors.affiliation: INRA, UR0588, Unité de Recherche Amélioration, Génétique et Physiologie Forestières, Centre de Recherche d'Orléans, Orléans Cedex 2, 45075, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Jorge
authors.affiliation: INRA, UR0588, Unité de Recherche Amélioration, Génétique et Physiologie Forestières, Centre de Recherche d'Orléans, Orléans Cedex 2, 45075, France.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Dowkiw
authors.affiliation: INRA, UR0588, Unité de Recherche Amélioration, Génétique et Physiologie Forestières, Centre de Recherche d'Orléans, Orléans Cedex 2, 45075, France.
authors.forename: Vanina
authors.initials: V
authors.lastname: Guerin
authors.affiliation: INRA, UR0588, Unité de Recherche Amélioration, Génétique et Physiologie Forestières, Centre de Recherche d'Orléans, Orléans Cedex 2, 45075, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Bourgait
authors.affiliation: Oak Ridge National Laboratory, PO Box 2008, MS-6422, Bldg. 1062, Rm 215, Oak Ridge, TN 37831-6422, USA.
authors.forename: Gerald A
authors.initials: GA
authors.lastname: Tuskan
authors.affiliation: Hudson Alpha Institute for Biotechnology, 601 Genome Way, Huntsville, AL 3508-2908, USA.
authors.forename: Jeremy
authors.initials: J
authors.lastname: Schmutz
authors.affiliation: INRA, UMR1165, UMR INRA/Université de Evry: Unité de Recherche en Génomique Végétale, Centre de Recherche de Versailles-Grignon, Evry Cedex, 91057, France.
authors.forename: Boulos
authors.initials: B
authors.lastname: Chalhoub
authors.affiliation: INRA, UR0588, Unité de Recherche Amélioration, Génétique et Physiologie Forestières, Centre de Recherche d'Orléans, Orléans Cedex 2, 45075, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Bastien
authors.affiliation: INRA, UMR1165, UMR INRA/Université de Evry: Unité de Recherche en Génomique Végétale, Centre de Recherche de Versailles-Grignon, Evry Cedex, 91057, France.
authors.forename: Patricia
authors.initials: P
authors.lastname: Faivre Rampant
mesh_terms: D001483:Base Sequence; D001487:Basidiomycota / Q000502:physiology; D001665:Binding Sites; D032461:Chromosomes, Plant / Q000235:genetics*; D003433:Crosses, Genetic; D060467:Disease Resistance / Q000235:genetics*; D017343:Genes, Plant / Q000235:genetics; D008040:Genetic Linkage; D056426:Genetic Loci / Q000235:genetics; D005819:Genetic Markers; D018745:Genome, Plant / Q000235:genetics*; D006239:Haplotypes / Q000235:genetics; D000090263:Leucine-Rich Repeat Proteins; D009711:Nucleotides / Q000378:metabolism*; D010641:Phenotype; D020161:Physical Chromosome Mapping; D010935:Plant Diseases / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D032107:Populus / Q000235:genetics*; D011506:Proteins / Q000235:genetics
chemical_list: D005819:Genetic Markers; D000090263:Leucine-Rich Repeat Proteins; D009711:Nucleotides; D011506:Proteins