title: Pale neurites, premature α-synuclein aggregates with centripetal extension from axon collaterals.
abstract: Progressive aggregation of α-synuclein (αS) from pale bodies (PBs) and extension from Lewy neurites (LNs) are candidate mechanisms for Lewy body (LB) formation. To identify how aggregation of αS is related to its extension along neurites, 60-µm-thick brainstem sections of Parkinson disease (PD) patients were prepared for three-dimensional (3D) reconstruction of αS-positive neurites with neurofilament (NF) and thiazin red (TR), a fluorochrome with an affinity to solid aggregates. This demonstrated 3D layering of αS surrounded by NF with the aggregates probed by TR in the center, corresponding to the eosinophilic core of mature LBs. This eosinophilic/TR-positive profile, characteristically absent in PBs, premature counterpart of LBs, was similarly absent in some LNs. We would like to refer these premature LNs as "pale neurites" (PNs). Their premature nature was evidenced by 3D fluoroprofiling with quantum dots (QDs) and subsequent electron microscopic identification (3D-oriented immunoelectron microscopy) as loosely packed αS (QDs)-positive filaments. Quantification of LNs, frequently extended around branching axons, demonstrated that LNs are initiated at axon collaterals to extend centripetally into proximal segments. This branching-oriented extension of αS is related to its selective predisposition to systems with highly divergent axons, preferentially affected in PD, which may explain barely somatotopic manifestations of PD.
authors.affiliation: Laboratory of Strucutural Neuropathology, Tokyo Metropolitan Institute of Medical Science, Setagaya, Tokyo, Japan.
authors.forename: Toshiro
authors.initials: T
authors.lastname: Kanazawa
authors.forename: Eijiro
authors.initials: E
authors.lastname: Adachi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Orimo
authors.forename: Ayako
authors.initials: A
authors.lastname: Nakamura
authors.forename: Hidehiro
authors.initials: H
authors.lastname: Mizusawa
authors.forename: Toshiki
authors.initials: T
authors.lastname: Uchihara
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001369:Axons / Q000378:metabolism / Q000473:pathology*; D001933:Brain Stem / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D002479:Inclusion Bodies / Q000378:metabolism / Q000473:pathology; D016631:Lewy Bodies / Q000378:metabolism / Q000473:pathology; D008297:Male; D016501:Neurites / Q000378:metabolism / Q000473:pathology*; D010300:Parkinson Disease / Q000378:metabolism / Q000473:pathology*; D011498:Protein Precursors / Q000378:metabolism; D051844:alpha-Synuclein / Q000378:metabolism*
chemical_list: D011498:Protein Precursors; D051844:alpha-Synuclein