title: Differentiation of Cuscuta chinensis and Cuscuta australis by HPLC-DAD-MS analysis and HPLC-UV quantitation.
abstract: Cuscuta chinensis and Cuscuta australis, the two botanical sources of the Chinese herbal medicine Tu-Si-Zi, were distinguished from each other based on qualitative and quantitative chemical analysis. By HPLC‑DAD‑MS, a total of 36 compounds were characterized from these two Cuscuta species, including 14 flavonoids, 17 quinic acid derivatives, and 5 lignans. In addition, HPLC‑UV was applied to determine seven major compounds (6 flavonoids plus chlorogenic acid) in 27 batches of Tu-Si-Zi. The results revealed that the amounts of the three classes of compounds varied significantly between the species. C. australis contained more flavonoids but less quinic acid derivatives and lignans than C. chinensis. Particularly, the amounts of kaempferol and astragalin in C. australis were remarkably higher than in C. chinensis. This finding could be valuable for the quality control of Tu-Si-Zi.
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, China.
authors.forename: Xianghui
authors.initials: X
authors.lastname: He
authors.forename: Wenzhi
authors.initials: W
authors.lastname: Yang
authors.forename: Min
authors.initials: M
authors.lastname: Ye
authors.forename: Qing
authors.initials: Q
authors.lastname: Wang
authors.forename: Dean
authors.initials: D
authors.lastname: Guo
mesh_terms: D002623:Chemistry Techniques, Analytical; D002851:Chromatography, High Pressure Liquid; D034182:Cuscuta / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D005419:Flavonoids / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D044949:Kaempferols / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D017705:Lignans / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D013058:Mass Spectrometry; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification; D011801:Quinic Acid / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D012639:Seeds / Q000737:chemistry
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D044949:Kaempferols; D017705:Lignans; D010936:Plant Extracts; D011801:Quinic Acid; C006552:kaempferol; C001579:astragalin