title: Immunohistochemical detection of Raf kinase inhibitor protein in normal cervical tissue and cervical cancer tissue.
abstract: Raf kinase inhibitor protein (RKIP) may be a suppressor of metastasis: RKIP levels are high in normal tissues, low in primary cancers and lowest or absent in metastatic cancers. This immunohisto chemistry study investigated RKIP protein levels in 250 clinical specimens of human cervical tissue and lymph node metastases (LNM) from 210 patients with normal cervical tissue, cervical intra-epithelial neoplasia (CIN), or cervical cancer with/without LNM. Thirty-nine (86.7%) of the 45 normal-tissue samples were RKIP-positive, six (13.3%) were RKIP-negative; 48/60 (80.0%) CIN samples were positive, 12 (20.0%) were negative; 47/105 (44.8%) cervical cancer tissue samples were positive, 58 (55.2%) were negative; only 7/40 (17.5%) LNM tissue samples were positive, 33 (82.5%) were negative. There was no significant correlation between RKIP positivity and clinical stage, microscopic subtype or pathological differentiation grade. RKIP positivity correlated inversely with LNM. RKIP may play a role in cervical-cancer genesis and metastasis; RKIP down-regulation was associated with metastatic disease in human cervical cancer.
authors.affiliation: Department of Obstetrics and Gynaecology, The First Affiliated Hospital, Harbin Medical University, Harbin City, China.
authors.forename: C J
authors.initials: CJ
authors.lastname: Hu
authors.forename: L
authors.initials: L
authors.lastname: Zhou
authors.forename: J
authors.initials: J
authors.lastname: Zhang
authors.forename: C
authors.initials: C
authors.lastname: Huang
authors.forename: G M
authors.initials: GM
authors.lastname: Zhang
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D002584:Cervix Uteri / Q000378:metabolism* / Q000473:pathology; D015536:Down-Regulation; D005260:Female; D005557:Formaldehyde; D006801:Humans; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis / Q000235:genetics / Q000473:pathology; D009367:Neoplasm Staging; D016612:Paraffin Embedding; D049488:Phosphatidylethanolamine Binding Protein / Q000235:genetics / Q000378:metabolism*; D016707:Tissue Fixation; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D002578:Uterine Cervical Dysplasia / Q000235:genetics / Q000378:metabolism* / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D049488:Phosphatidylethanolamine Binding Protein; D005557:Formaldehyde