title: Proteomic analyses of the Xiphophorus Gordon-Kosswig melanoma model.
abstract: Interspecies hybridization between the platyfish X. maculatus Jp 163 A, and the swordtail X. helleri (Sarabia), generates F(1) hybrids with pronounced melanin pigmentation. Backcrossing of F(1) hybrids with the X. helleri parent results in 25% of progeny that will spontaneously develop melanoma. We have applied proteomic methods to this Gordon-Kosswig (G-K) melanoma model to identify candidate proteins that exhibit modulated expression in fin tissue due to interspecies hybridization and progression of hybrid tissues to spontaneous melanoma. Difference Gel Electrophoresis (DIGE) was used to minimize the variability commonly observed in quantitative analyses of comparative protein samples. Following identification of up- or down-regulated protein expression by DIGE, candidate protein spots were identified by mass spectrometric sequencing. Several protein expression differences displayed in interspecies hybrids were identified and compared to distinct differences that occur upon backcrossing and progression to melanoma. These studies are important for the identification of distinct biochemical pathways involved in the variety of Xiphophorus interspecies hybrid tumor models.
authors.affiliation: Department of Chemistry & Biochemistry, Texas State University-San Marcos, 601 University Dr., San Marcos, TX 78666, USA.
authors.forename: Amy N
authors.initials: AN
authors.lastname: Perez
authors.forename: Lee
authors.initials: L
authors.lastname: Oehlers
authors.forename: Shelia J
authors.initials: SJ
authors.lastname: Heater
authors.forename: Rachell E
authors.initials: RE
authors.lastname: Booth
authors.forename: Ronald B
authors.initials: RB
authors.lastname: Walter
authors.forename: Wendi M
authors.initials: WM
authors.lastname: David
mesh_terms: D000595:Amino Acid Sequence; D058500:Animal Fins / Q000378:metabolism / Q000473:pathology; D000818:Animals; D002678:Chimera / Q000235:genetics / Q000378:metabolism; D003532:Cyprinodontiformes / Q000235:genetics / Q000378:metabolism*; D018450:Disease Progression; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D029941:Fish Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D007178:Inbreeding; D008546:Melanoma, Experimental / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008969:Molecular Sequence Data; D010455:Peptides / Q000235:genetics / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D029941:Fish Proteins; D010455:Peptides