title: [c-SRC knockdown decreases phosphorylated STAT3 expression and viability of HeLa cells].
abstract: The present study was to determine the effect of c-SRC on the viability of human cervical cancer HeLa cells and the expression of phosphorylated signal transducer and activator of transcription-3 (p-STAT3) of the cell. Post-transfection of c-SRC RNA interference vector, RT-PCR and Western blot were utilized to observe the contents of c-SRC mRNA and protein, respectively, in HeLa cells. The MTT was used to observe the viability of the cells. Cell cycle was observed by flow cytometry. The content of p-STAT3 in the cells was also investigated after knockdown of c-SRC. Knockdown of c-SRC significantly decreased the contents of c-SRC mRNA and protein in the cells. The viability of the cells decreased by 23.1%, 29.3%, 38.6% and 45.0% (all P < 0.05), respectively, after the cells were transfected with c-SRC RNA interference vector for 24, 48, 72, and 96 h. The number of S-phase cells decreased by 5.6%, 10.0%, 15.2% and 19.9% (all P < 0.05), respectively, after transfection of c-SRC RNA interference vector for 24, 48, 72, and 96 h. The content of p-STAT3 also decreased when c-SRC was knockdowned. Compared with the control group, after treatment of HeLa cells with STAT3 inhibitor Piceatannol for 24, 48, 72, and 96 h, the cell viability decreased by 23.8%, 29.7%, 37.3% and 45.4% (all P < 0.05), respectively, while increase of c-SRC content could not reverse the inhibitory effect. These results suggest that the inhibited viability of HeLa cells caused by knockdown of c-SRC is associated with the decreased content of p-STAT3 protein.
authors.affiliation: Department of Physiology, Nanchang University, Nanchang, China.
authors.forename: Jia-Xiang
authors.initials: JX
authors.lastname: Chen
authors.forename: Lin-Lin
authors.initials: LL
authors.lastname: Xu
authors.forename: Sheng-Jiao
authors.initials: SJ
authors.lastname: Wu
authors.forename: Hong-Yu
authors.initials: HY
authors.lastname: Liu
authors.forename: Jing-Lei
authors.initials: JL
authors.lastname: Wang
authors.forename: Ting
authors.initials: T
authors.lastname: Zou
mesh_terms: D002470:Cell Survival; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D016391:Genes, src / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D010766:Phosphorylation; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D050796:STAT3 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
chemical_list: D012333:RNA, Messenger; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human