title: Differences in two-dimensional gel electrophoresis patterns of skeletal muscle myosin light chain isoforms between Bos taurus, Sus scrofa and selected poultry species.
abstract: BACKGROUND
  In this study the interspecies differences in two-dimensional electrophoresis patterns of skeletal muscle myosin light chain (MLC) isoforms between Bos taurus (cattle), Sus scrofa (pig), Gallus gallus (chicken), Meleagris gallopavo (turkey), Anas platyrhynchos (duck) and Anser anser (goose) were characterised on the basis of specific properties of MLCs associated with their structure and mobility in gel.
  
  
  RESULTS
  Two-dimensional electrophoresis separations revealed species-specific differences in the molecular weight and pI of individual MLC isoforms (MLC1f, MLC2f and MLC3f). In the case of closely related animal species such as goose and duck or turkey and chicken, significant differences occurred in MLC1f. For MLC2f, differences between cattle and turkey and between pig and chicken were around 1 and 0.3 kDa respectively. It appeared from the comparison of amino acid sequences that even MLCs with only 2% difference in sequences have different electrophoretic mobilities.
  
  
  CONCLUSIONS
  Interspecies differences in skeletal MLC isoforms appeared between cattle, pig, chicken, turkey, duck and goose. The slight changes observed in the course of the aging process confirmed that these proteins are relatively little susceptible to proteolytic enzymes during meat aging.
authors.affiliation: Institute of Meat Technology, Poznań University of Life Sciences, Poznań, Poland. magdalena.montowska@gmail.com
authors.forename: Magdalena
authors.initials: M
authors.lastname: Montowska
authors.forename: Edward
authors.initials: E
authors.lastname: Pospiech
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D030161:Avian Proteins / Q000737:chemistry / Q000378:metabolism; D002417:Cattle; D030562:Databases, Protein; D004044:Dietary Proteins / Q000378:metabolism; D007526:Isoelectric Point; D008460:Meat / Q000032:analysis*; D008462:Meat-Packing Industry / Q000379:methods; D008970:Molecular Weight; D018482:Muscle, Skeletal / Q000378:metabolism*; D018994:Myosin Light Chains / Q000737:chemistry* / Q000378:metabolism*; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D010449:Peptide Mapping; D011200:Poultry; D020033:Protein Isoforms / Q000737:chemistry / Q000378:metabolism; D016415:Sequence Alignment; D013045:Species Specificity; D034421:Sus scrofa; D057825:Two-Dimensional Difference Gel Electrophoresis
chemical_list: D030161:Avian Proteins; D004044:Dietary Proteins; D018994:Myosin Light Chains; D010446:Peptide Fragments; D020033:Protein Isoforms