title: Fabrication of dynamic self-assembled monolayers for cell migration and adhesion studies.
abstract: How cells interact with the extracellular matrix (ECM) is important for a number of fundamental -processes in cell biology. However, the ECM is highly complex and in order to simplify the matrix for cell biological studies, it has been modeled with self-assembled monolayers (SAMs) of alkanethiolates on gold substrates. In this chapter, we outline procedures to create dynamic surfaces by functionalizing SAMs. SAMs based on quinone, oxyamine, and alcohol-terminated thiols were used to immobilize cell adhesive peptides with spatial control. Cells were seeded to these surfaces to provide cell co-culture -patterns suitable for biological studies.
authors.forename: Nathan P
authors.initials: NP
authors.lastname: Westcott
authors.forename: Muhammad N
authors.initials: MN
authors.lastname: Yousaf
mesh_terms: D016475:3T3 Cells; D000438:Alcohols / Q000737:chemistry; D000447:Aldehydes / Q000737:chemistry; D000818:Animals; D016227:Benzoquinones / Q000737:chemistry; D002448:Cell Adhesion; D002465:Cell Movement; D018920:Coculture Techniques / Q000379:methods*; D004563:Electrochemistry; D055767:Immobilized Proteins / Q000737:chemistry; D007202:Indicators and Reagents / Q000737:chemistry; D051379:Mice; D010091:Oximes / Q000737:chemistry; D010455:Peptides / Q000737:chemistry; D011327:Printing; D013499:Surface Properties
chemical_list: D000438:Alcohols; D000447:Aldehydes; D016227:Benzoquinones; D055767:Immobilized Proteins; D007202:Indicators and Reagents; D010091:Oximes; D010455:Peptides; C004532:quinone