title: Decreasing the configurational entropy and the hydrophobicity of EBV-derived peptide 11389 increased its antigenicity, immunogenicity and its ability of inducing IL-6.
abstract: Peptide 11389 from CD21-binding region of EBV-gp350/220 protein binds to PBMCs inducing IL-6 expression and inhibiting EBV-binding to PBMCs. In addition, anti-peptide 11389 antibodies recognize EBV-infected cells and inhibit both EBV infection and IL-6 production in PBMCs. We have postulated that native structure stabilization of peptide 11389 sequence can increase its biological activity. The strategy was to modify its sequence to restrict the number of structures that peptide 11389 could acquire in solution (decreasing peptide's configurational entropy) and to weaken the non-relevant intermolecular interactions (decreasing its hydrophobicity), preserving CD21-interacting residues and structure as displayed in the native protein. Thirteen analog peptides were designed and synthesized; most of them were monomers containing an intra-chain disulfide bridge. Analog peptides 34058, 34060, 34061, 34296, 34298, 34299 and 34300 inhibited EBV invasion of PBMCs. Peptides 34059, 34060, 34295 and 34297 induced IL-6 levels in PBMCs (EC50=3.4, 3.3, 0.5, 0.5 μM, respectively) at higher potency than peptide 11389 (EC50=5.8 μM). Peptides 34057, 34059, 34060, 34301 and 34302 interacted with anti-EBV antibodies with affinities from 3 to 50 times higher than peptide 11389. Most of analog peptides were highly immunogenic and elicited antibodies that cross-react with EBV. In conclusion, we have designed peptides displaying higher biological activity than peptide 11389.
authors.affiliation: Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, 725 N. Wolfe Street WBSB 603, Baltimore, MD 21205, USA. murquiz1@jhmi.edu
authors.forename: Mauricio
authors.initials: M
authors.lastname: Urquiza
authors.forename: Tatiana
authors.initials: T
authors.lastname: Guevara
authors.forename: Cristina
authors.initials: C
authors.lastname: Rodriguez
authors.forename: Johanna
authors.initials: J
authors.lastname: Melo-Cardenas
authors.forename: Magnolia
authors.initials: M
authors.lastname: Vanegas
authors.forename: Manuel E
authors.initials: ME
authors.lastname: Patarroyo
mesh_terms: D000595:Amino Acid Sequence; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000276:immunology; D000956:Antigens, Viral / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D004305:Dose-Response Relationship, Drug; D019277:Entropy; D004854:Herpesvirus 4, Human / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D015850:Interleukin-6 / Q000096:biosynthesis / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology* / Q000494:pharmacology; D011487:Protein Conformation; D017464:Receptors, Complement 3d / Q000276:immunology / Q000378:metabolism; D013329:Structure-Activity Relationship; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D053586:Virus Internalization / Q000187:drug effects
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D015850:Interleukin-6; D010455:Peptides; D017464:Receptors, Complement 3d; D014764:Viral Proteins