title: Metabolic profiling of strawberry grape ( Vitis × labruscana cv. 'Isabella') components by nuclear magnetic resonance (NMR) and evaluation of their antioxidant and antiproliferative properties.
abstract: In the assessment of the antioxidant properties of edible plants, the widely consumed Vitis × labruscana cv. 'Isabella', known in Italy as "fragola" (strawberry) grape, was of interest. Phenol and flavonoid contents of the methanolic extracts of peel, pulp, seed, leaf, and stalk components of the plant were determined. The metabolic profile of the extracts was performed by 1D and 2D NMR. Quantitative analysis, obtained in the presence of 0.01% of internal standard trimethylsilyl propionate, evidenced the presence of catechins in both stalk and seed extracts, whereas caffeic acid and quercetin were the main metabolites of the leaf extract. Furthermore, the extracts were tested for their radical scavenging and reducing capacities by measuring their capacity to scavenge DPPH(•) and ABTS(•+) and to reduce Fe(III) and Mo(VI) salts. The antioxidant efficacy of the extracts in cell-free systems and their antiproliferative activity toward HepG2 and A549 cells were also evaluated. Seed and stalk components are able to reduce by 39.6 and 40.6%, respectively, the amount of the metabolically active HepG2 cells after only 24 h of exposure.
authors.affiliation: Dipartimento di Scienze della Vita, Seconda Università degli Studi di Napoli, Caserta, Italy. severina.pacifico@unina2.it
authors.forename: Severina
authors.initials: S
authors.lastname: Pacifico
authors.forename: Brigida
authors.initials: B
authors.lastname: D'Abrosca
authors.forename: Monica
authors.initials: M
authors.lastname: Scognamiglio
authors.forename: Marialuisa
authors.initials: M
authors.lastname: Gallicchio
authors.forename: Nicoletta
authors.initials: N
authors.lastname: Potenza
authors.forename: Simona
authors.initials: S
authors.lastname: Piccolella
authors.forename: Aniello
authors.initials: A
authors.lastname: Russo
authors.forename: Pietro
authors.initials: P
authors.lastname: Monaco
authors.forename: Antonio
authors.initials: A
authors.lastname: Fiorentino
mesh_terms: D000975:Antioxidants / Q000032:analysis* / Q000378:metabolism / Q000494:pharmacology; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D055442:Metabolome; D010936:Plant Extracts / Q000032:analysis* / Q000378:metabolism / Q000494:pharmacology*; D027843:Vitis / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000975:Antioxidants; D010936:Plant Extracts