title: [Effects of bicyclol on renal PAI-1 expression in rats with uniliteral ureteral obstruction].
abstract: OBJECTIVE
  To explore the protective effects of bicyclol against renal interstitial fibrosis and possible mechanisms of the protection.
  
  
  METHODS
  Eighty-one Sprague-Dawley (SD) rats were randomly assigned to a sham-operated group and UUO groups with and without bicyclol treatment. A rat model of renal interstitial fibrosis was prepared by unilateral ureteral obstruction (UUO). Renal tissues were examined by hematoxylin & eosin and Masson staining on 7, 14 and 21 days. Immunhistochemistry was used for determining plasminogen activator inhibitor-1(PAI-1) expression in the renal interstitium. PAI-1 mRNA expression in renal tissues was semi-quantitatively determined by reverse transcription-polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  The relative areas of renal interstitial fibrosis in the bicyclol-treated UUO group 7, 14 and 21 days after operation were (9.6 ± 0.6)%, (16.8 ± 0.8)% and (33.6 ± 1.6)% respectively, which were significantly lower than those in the untreated UUO group [13.0 ± 0.7)%, (25.8 ± 1.5)% and (53.2 ± 2.5)% respectively] (P<0.05). The levels of protein and mRNA expression of PAI-1 in the bicyclol-treated UUO group decreased significantly compared with those in the untreated UUO group 7, 14 and 21 days after operation (P<0.05).
  
  
  CONCLUSIONS
  Bicyclol can alleviate renal interstitial injury and renal interstitial fibrosis caused by UUO in rats, possibly through a downregulation of renal PAI-1 expression.
authors.affiliation: Department of Internal Medicine, Xinxiang Medical College, Weihui, Henan 453100, China.
authors.forename: Yan-Hong
authors.initials: YH
authors.lastname: Liu
authors.forename: Zi-Ming
authors.initials: ZM
authors.lastname: Han
mesh_terms: D000818:Animals; D001713:Biphenyl Compounds / Q000494:pharmacology* / Q000627:therapeutic use; D005355:Fibrosis; D007668:Kidney / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D017395:Plasminogen Activator Inhibitor 1 / Q000032:analysis* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D014517:Ureteral Obstruction / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology
chemical_list: D001713:Biphenyl Compounds; D017395:Plasminogen Activator Inhibitor 1; D012333:RNA, Messenger; C477843:bicyclol