title: Electrodeposition of chitosan-glucose oxidase biocomposite onto Pt-Pb nanoparticles modified stainless steel needle electrode for amperometric glucose biosensor.
abstract: A glucose biosensor was fabricated by electrodepositing chitosan (CS)-glucose oxidase(GOD) biocomposite onto the stainless steel needle electrode (SSN electrode) modified by Pt-Pb nanoparticles (Pt-Pb/SSN electrode). Firstly, Pt-Pb nanoparticles were deposited onto the SSN electrode and then CS-GOD biocomposite was co-electrodeposited onto the Pt-Pb/SSN electrode in a mixed solution containing p-benzoquinone (p-BQ), CS and GOD. The electrochemical results showed that the Pt-Pb nanoparticles can accelerate the electron transfer and improve the effective surface area of the SSN electrode. As a result, the detection range of the proposed biosensor was from 0.03 to 9 mM with a current sensitivity of 0.4485 μA/mM and a response time of 15 s. The Michaelis constant value was calculated to be 4.9837 mM. The cell test results indicated that the electrodes have a low cytotoxicity. This work provided a suitable technology for the fabrication of the needle-type glucose biosensor.
authors.affiliation: School of Materials Science and Engineering, Tianjin University, Tianjin, 300072, China.
authors.forename: Meiqing
authors.initials: M
authors.lastname: Guo
authors.forename: Haidong
authors.initials: H
authors.lastname: Fang
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Yang
authors.forename: Xinhua
authors.initials: X
authors.lastname: Xu
mesh_terms: D015374:Biosensing Techniques; D048271:Chitosan / Q000737:chemistry*; D004563:Electrochemistry; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry*; D007854:Lead / Q000737:chemistry*; D053768:Metal Nanoparticles; D008855:Microscopy, Electron, Scanning; D009339:Needles; D010984:Platinum / Q000737:chemistry*; D013193:Stainless Steel
chemical_list: D013193:Stainless Steel; D007854:Lead; D010984:Platinum; D048271:Chitosan; D005949:Glucose Oxidase; D005947:Glucose