title: Large-scale sequencing of normalized full-length cDNA library of soybean seed at different developmental stages and analysis of the gene expression profiles based on ESTs.
abstract: Although GenBank has now covered over 1,400,000 expressed sequence tags (ESTs) from soybean, most ESTs available to the public have been derived from tissues or environmental conditions rather than developing seeds. It is absolutely necessary for annotating the molecular mechanisms of soybean seed development to analyze completely the gene expression profiles of its immature seed at various stages. Here we have constructed a full-length-enriched cDNA library comprised of a total of 45,408 cDNA clones which cover various stages of soybean seed development. Furthermore, we have sequenced from 5' ends of these clones, 36,656 ESTs were obtained in the present study. These EST sequences could be categorized into 27,982 unigenes, including 22,867 contigs and 5,115 singletons, among which 27,931 could be mapped onto soybean 20 chromosome sequences. Comparative genomic analysis with other plants has revealed that these unigenes include lots of candidate genes specific to dicot, legume and soybean. Approximately 1,789 of these unigenes currently show no homology to known soybean sequences, suggesting that many represent mRNAs specifically expressed in seeds. Novel abundant genes involved in the oil synthesis have been found in this study, may serve as a valuable resource for soybean seed improvement.
authors.affiliation: Institute of Oil Crops, Key Laboratory of Oil Crop Biology of the Ministry of Agriculture, Chinese Academy of Agricultural Sciences, Wuhan 430062, China.
authors.forename: Ai-Hua
authors.initials: AH
authors.lastname: Sha
authors.forename: Chen
authors.initials: C
authors.lastname: Li
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Yan
authors.forename: Zhi-Hui
authors.initials: ZH
authors.lastname: Shan
authors.forename: Xin-An
authors.initials: XA
authors.lastname: Zhou
authors.forename: Mu-Lan
authors.initials: ML
authors.lastname: Jiang
authors.forename: Han
authors.initials: H
authors.lastname: Mao
authors.forename: Bo
authors.initials: B
authors.lastname: Chen
authors.forename: Xia
authors.initials: X
authors.lastname: Wan
authors.forename: Wen-Hui
authors.initials: WH
authors.lastname: Wei
mesh_terms: D001483:Base Sequence; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D015723:Gene Library; D023281:Genomics; D008969:Molecular Sequence Data; D012639:Seeds / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013025:Glycine max / Q000235:genetics* / Q000254:growth & development; D013045:Species Specificity