title: Four independent molecular prion protein parameters for discriminating new cases of C, L, and h bovine spongiform encephalopathy in cattle.
abstract: In anticipation of the emergence of more variants of bovine spongiform encephalopathy (BSE), a semiquantitative display of the following four independent molecular diagnostic prion parameters was designed: N terminus, proteinase K (PK) resistance, glycoprofile, and mixed population. One H BSE case, three L BSE cases, six C BSE cases, and one unusual classical BSE (C BSE) case are reported.
authors.affiliation: Central Veterinary Institute of Wageningen UR, P.O. Box 65, 8200 AB Lelystad, The Netherlands. jan.langeveld@wur.nl
authors.forename: Jan P M
authors.initials: JP
authors.lastname: Langeveld
authors.forename: Jo H F
authors.initials: JH
authors.lastname: Erkens
authors.forename: Ines
authors.initials: I
authors.lastname: Rammel
authors.forename: Jorg G
authors.initials: JG
authors.lastname: Jacobs
authors.forename: Aart
authors.initials: A
authors.lastname: Davidse
authors.forename: Fred G
authors.initials: FG
authors.lastname: van Zijderveld
authors.forename: Alex
authors.initials: A
authors.lastname: Bossers
authors.forename: Hermann
authors.initials: H
authors.lastname: Schildorfer
mesh_terms: D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D002417:Cattle; D016643:Encephalopathy, Bovine Spongiform / Q000175:diagnosis*; D019286:Endopeptidase K / Q000378:metabolism; D000939:Epitopes / Q000276:immunology; D011328:Prions / Q000737:chemistry / Q000145:classification* / Q000276:immunology / Q000302:isolation & purification*
chemical_list: D002241:Carbohydrates; D000939:Epitopes; D011328:Prions; D019286:Endopeptidase K