title: Purified autoantibodies against glucose-regulated protein 78 (GRP78) promote apoptosis and decrease invasiveness of ovarian cancer cells.
abstract: Circulating glucose-regulated protein 78 (GRP78) autoantibodies represent potential biomarker of epithelial ovarian cancer. However there is relatively limited identification of these antibodies in response to ovarian cancer. Here, we were interested in characterization of these antibodies and into their role in tumor development. We first purified GRP78 autoantibodies from sera of patients with ovarian cancer, and then tested them on SKOV-3 ovarian cancer cell line. A decrease of invasion and an increase of H(2)O(2)-induced apoptosis of SKOV-3 cells were observed, suggesting their protective role against ovarian cancer cells.
authors.affiliation: Department of Gynecology-Obstetrics, 30 boulevard de la Cluse, Faculty of Medicine, Geneva 14, Switzerland. marie.cohen@hcuge.ch
authors.forename: Marie
authors.initials: M
authors.lastname: Cohen
authors.forename: Patrick
authors.initials: P
authors.lastname: Petignat
mesh_terms: D017209:Apoptosis / Q000276:immunology; D001323:Autoantibodies / Q000097:blood / Q000276:immunology; D000077216:Carcinoma, Ovarian Epithelial; D045744:Cell Line, Tumor; D000091342:Endoplasmic Reticulum Chaperone BiP; D005260:Female; D006360:Heat-Shock Proteins / Q000276:immunology*; D006801:Humans; D009361:Neoplasm Invasiveness / Q000276:immunology; D009375:Neoplasms, Glandular and Epithelial / Q000097:blood / Q000276:immunology / Q000473:pathology; D010051:Ovarian Neoplasms / Q000097:blood / Q000276:immunology / Q000473:pathology
chemical_list: D001323:Autoantibodies; D000091342:Endoplasmic Reticulum Chaperone BiP; C000717247:HSPA5 protein, human; D006360:Heat-Shock Proteins