title: A novel multiplex PCR method for detecting the major clonal complexes of MRSA in nasal isolates from a Pennsylvania hospital.
abstract: A novel multiplex PCR was developed which targeted virulence genes associated with the major clonal complexes (CCs) of healthcare- and community-associated methicillin-resistant Staphylococcus aureus (MRSA) in the USA. Most isolates (40/66) were identified as CC 5, while remaining isolates represented CCs 1, 8, 30, 45, 59, 133, and five isolates were not S. aureus.
authors.affiliation: Department of Food Science, The Pennsylvania State University, University Park, PA 16802, USA. nathan.schwalm@yale.edu
authors.forename: Nathan D
authors.initials: ND
authors.lastname: Schwalm
authors.forename: Bindhu
authors.initials: B
authors.lastname: Verghese
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Knabel
mesh_terms: D001483:Base Sequence; D015415:Biomarkers; D017714:Community-Acquired Infections / Q000175:diagnosis*; D003428:Cross Infection / Q000175:diagnosis*; D005838:Genotype; D006761:Hospitals; D055624:Methicillin-Resistant Staphylococcus aureus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D009666:Nose / Q000382:microbiology*; D010414:Pennsylvania; D014481:United States
chemical_list: D015415:Biomarkers