title: [Determination of five iridoid glycosides in Phlomis younghusbandii by HPLC].
abstract: OBJECTIVE
  To develop an RP-HPLC method for the determination of five iridoid glycosides in Phlomis younghusbandii.
  
  
  METHODS
  HPLC analysis was performed on a Symmetry C18 (4.6 mm x 150 mm, 5 microm, Waters) column eluted with acetonitrile (A) and water (B) in gradient elution. The gradient program was as follows: 0-5 min kept 7% A; 5-10 min changed to 12% A; 10-40 min kept 12% A. The flow rate was 1.0 mL x min(-1). The column temperature was 20 degrees and the detection wavelength was 235 nm.
  
  
  RESULTS
  The linear ranges of sesamoside, shanzhiside methyl ester, 7, 8-dehydropenstemoside, penstemoside and 8-O-acetylshanzhiside methyl ester were 0.050-0.650 (r = 0.999 3), 0.050-0.350 (r = 0.999 5), 0.040-0.280 (r = 0.999 4), 0.010-0.070 (r = 0.999 6), 0.040-0. 280 (r = 0.999 7) g x L(-1), respectively. The average recoveries (n = 6) of them were all between 96% and 104%, RSD < 5.0%.
  
  
  CONCLUSIONS
  The method is simple, accurate, repeatable and stable, which can be used for quality control of P. younghusbandii.
authors.affiliation: Department of Pharmacy, Lanzhou General Hospital of PLA, Lanzhou 730050, China. limaox2005@yahoo.com.cn
authors.forename: Maoxing
authors.initials: M
authors.lastname: Li
authors.forename: Chao
authors.initials: C
authors.lastname: Zhang
authors.forename: Lili
authors.initials: L
authors.lastname: Wei
authors.forename: Pengcheng
authors.initials: P
authors.lastname: Fan
authors.forename: Quanlong
authors.initials: Q
authors.lastname: Zhang
authors.forename: Zhengping
authors.initials: Z
authors.lastname: Jia
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D057889:Iridoid Glycosides / Q000032:analysis*; D031345:Phlomis / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results
chemical_list: D004365:Drugs, Chinese Herbal; D057889:Iridoid Glycosides