title: Analyses of human papillomavirus genotypes and viral loads in anogenital warts.
abstract: Condylomata acuminata (genital warts) are the most common sexually transmitted viral diseases. These lesions are caused by infection with mucosal human papillomaviruses (HPVs). However, there is limited information on HPV strain distribution involved in the molecular pathogenesis of these lesions. To address this, the strain prevalence and the frequency of multiple HPV infections were determined in wart tissue obtained from 31 patients attending a wart clinic. These lesions were bisected and subjected to parallel DNA and mRNA extractions. HPV-type prevalence and incidence of multiple infections were determined by the Roche Linear Array assay. qPCR compared HPV 6, 11, 16, and 18 viral loads and RT-qPCR measured HPV 6 and 11 E6 genomic expression levels. Seventy-one percent of these samples were infected with multiple HPVs. Only one sample was negative for HPV 6 or 11 DNA. Forty-eight percent of samples were positive for a high risk (oncogenic) HPV. The results show that multiple infections in tissue are frequent and the subsequent analysis of HPV 6 and 11 E6 DNA viral loads suggested that other HPVs could be causing lesions. Further analysis of HPV 6/11 E6 mRNA levels showed that there was no discernable relationship between HPV 6 E6 DNA viral load and relative HPV 6 or 11 E6 mRNA levels thereby questioning the relevance of viral load to lesion causality.
authors.affiliation: Department of Pathology, University of Cambridge, Cambridge, UK. slrb2@cam.ac.uk
authors.forename: Siolian L R
authors.initials: SL
authors.lastname: Ball
authors.forename: David M
authors.initials: DM
authors.lastname: Winder
authors.forename: Katie
authors.initials: K
authors.lastname: Vaughan
authors.forename: Nashat
authors.initials: N
authors.lastname: Hanna
authors.forename: Jonathan
authors.initials: J
authors.lastname: Levy
authors.forename: Jane C
authors.initials: JC
authors.lastname: Sterling
authors.forename: Margaret A
authors.initials: MA
authors.lastname: Stanley
authors.forename: Peter K C
authors.initials: PK
authors.lastname: Goon
mesh_terms: D003218:Condylomata Acuminata / Q000453:epidemiology* / Q000473:pathology / Q000821:virology*; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D005838:Genotype; D006801:Humans; D015994:Incidence; D008297:Male; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D015995:Prevalence; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D019562:Viral Load
chemical_list: D004279:DNA, Viral; D012333:RNA, Messenger; D012367:RNA, Viral