title: Multi-locus sequence typing of Salmonella enterica subsp. enterica serovar Enteritidis strains in Japan between 1973 and 2004.
abstract: Salmonella enterica subsp. enterica serovar Enteritidis (S. Enteritidis) was responsible for a worldwide pandemic during the 1980s and 1990s; however, changes in the dominant lineage before and after this event remain unknown. This study determined S. Enteritidis lineages before and after this pandemic event in Japan using multilocus sequence typing (MLST). Thirty S. Enteritidis strains were collected in Japan between 1973 and 2004, consisting of 27 human strains from individual episodes, a bovine strain, a liquid egg strain and an eggshell strain. Strains showed nine phage types and 17 pulsed-field profiles with pulsed-field gel electrophoresis. All strains had homologous type 11 sequences without any nucleotide differences in seven housekeeping genes. These MLST results suggest that S. Enteritidis with the diversities revealed by phage typing and pulsed-field profiling has a highly clonal population. Although type 11 S. Enteritidis may exhibit both pleiotropic surface structure and pulsed-field type variation, it is likely to be a stable lineage derived from an ancestor before the 1980s and/or 1990s pandemic in Japan.
authors.affiliation: Division of Pathology and Bacteriology, Fukuoka Institute of Health and Environmental Sciences, 39 Mukaizano, Dazaifu, Fukuoka 818-0135, Japan.
authors.forename: Tamie
authors.initials: T
authors.lastname: Noda
authors.forename: Koichi
authors.initials: K
authors.lastname: Murakami
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Asai
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Etoh
authors.forename: Tomoe
authors.initials: T
authors.lastname: Ishihara
authors.forename: Toshiro
authors.initials: T
authors.lastname: Kuroki
authors.forename: Kazumi
authors.initials: K
authors.lastname: Horikawa
authors.forename: Shuji
authors.initials: S
authors.lastname: Fujimoto
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D001434:Bacteriophage Typing; D001483:Base Sequence; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D004531:Eggs; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D007564:Japan; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing; D058873:Pandemics; D010802:Phylogeny; D011200:Poultry; D011201:Poultry Diseases / Q000382:microbiology*; D012480:Salmonella Infections / Q000382:microbiology*; D012481:Salmonella Infections, Animal / Q000382:microbiology*; D012477:Salmonella enteritidis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial