title: A Sexually Transmitted Disease (STD) DNA chip for the diagnosis of genitourinary infections.
abstract: The results of an investigation aimed at the development of a DNA chip for the detection of genitourinary infections are described. Through analysis of over 35,000 clinical cases, 14 pathogens which are most abundantly found among Koreans were selected and candidate sequences for capture probes were accordingly chosen by considering their sequences and β-globin house-keeping gene. Among this group, the most suitable capture probe sequences were selected by employing repeated chip tests in which they are immobilized on a glass chip by using a recently developed novel gold nanoparticles-based method. A multiplex PCR method was established to generate fluorescence-labeled sequences for all 14 pathogens along with the β-globin gene. By using optimized hybridization conditions, the final chip was constructed and employed to diagnose reliably both single and multiple infections in clinical human samples for 14 target pathogens. The results show that the novel chip methodology serves as a highly reliable and convenient tool for the diagnosis of Sexually Transmitted Diseases (STDs). Furthermore, this study has its great significance in that it demonstrates the entire process from statistical analysis of a large number of clinical cases to the final development of STD DNA chip just ready to be applied or commercialized in the clinical diagnostic field.
authors.affiliation: Department of Chemical & Biomolecular Engineering (BK 21 program), KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.
authors.forename: Won-Young
authors.initials: WY
authors.lastname: Chung
authors.forename: Ye Lim
authors.initials: YL
authors.lastname: Jung
authors.forename: Ki Soo
authors.initials: KS
authors.lastname: Park
authors.forename: Cheulhee
authors.initials: C
authors.lastname: Jung
authors.forename: Sung Chul
authors.initials: SC
authors.lastname: Shin
authors.forename: Sang-Joon
authors.initials: SJ
authors.lastname: Hwang
authors.forename: Dae-Yeon
authors.initials: DY
authors.lastname: Cho
authors.forename: Sun Ho
authors.initials: SH
authors.lastname: Cha
authors.forename: Si Kyu
authors.initials: SK
authors.lastname: Lim
authors.forename: Hyun Gyu
authors.initials: HG
authors.lastname: Park
mesh_terms: D004867:Equipment Design; D005260:Female; D005456:Fluorescent Dyes; D006046:Gold; D006801:Humans; D008297:Male; D053768:Metal Nanoparticles; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods*; D016133:Polymerase Chain Reaction; D056910:Republic of Korea; D012749:Sexually Transmitted Diseases / Q000175:diagnosis* / Q000382:microbiology / Q000469:parasitology / Q000821:virology
chemical_list: D005456:Fluorescent Dyes; D006046:Gold