title: Langerhans cells in periodontal disease of HIV- and HIV+ patients undergoing highly active antiretroviral therapy.
abstract: The aim of this study was to assess and compare quantitatively the presence of S100+ Langerhans cells (LC) by immunochemistry techniques in HIV+ and HIV- gingivitis and periodontitis subjects. Additionally, it aimed to evaluate the correlation among densities of these cells with CD4+ and CD8+ T cells, and viral load levels in HIV+ subjects, all using Highly Active Antiretroviral Therapy (HAART). The samples were allocated into four groups: 1) 15 subjects with moderate chronic periodontitis (MCP), HIV+; 2) 15 subjects with MCP, HIV-; 3) 10 subjects with gingivitis (G), HIV+; and 4) 10 subjects with G, HIV-. The S100+ cells were assessed in the pocket epithelium, gingival epithelium, and lamina propria. A statistically significant increase of total S100+ cells in HIV+ periodontitis subjects was observed in relation to HIV- periodontitis subjects. No increase of S100+ cells with increased inflammation was observed. No statistically significant correlation among S100+ cells and blood levels of CD4, CD8, and viral load was observed. In conclusion, the use of HAART can aid in achieving viral loads, and it is suggested that it may prevent the destruction of the LC.
authors.affiliation: Department of Periodontology, Dental School, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil. takeshisegundo@hotmail.com
authors.forename: Takeshi Kato
authors.initials: TK
authors.lastname: Segundo
authors.forename: Giovanna Ribeiro
authors.initials: GR
authors.lastname: Souto
authors.forename: Ricardo Alves
authors.initials: RA
authors.lastname: Mesquita
authors.forename: Fernando Oliveira
authors.initials: FO
authors.lastname: Costa
mesh_terms: D000328:Adult; D023241:Antiretroviral Therapy, Highly Active; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002452:Cell Count; D005260:Female; D005891:Gingivitis / Q000276:immunology / Q000473:pathology*; D015658:HIV Infections / Q000188:drug therapy / Q000276:immunology / Q000473:pathology*; D006801:Humans; D007801:Langerhans Cells / Q000187:drug effects / Q000276:immunology / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D010518:Periodontitis / Q000276:immunology / Q000473:pathology*; D009418:S100 Proteins / Q000032:analysis; D018709:Statistics, Nonparametric; D013601:T-Lymphocytes; D019562:Viral Load
chemical_list: D009418:S100 Proteins