title: Induced production of N-formyl alkaloids from Aspergillus fumigatus by co-culture with Streptomyces peucetius.
abstract: Co-culture of the fungus Aspergillus fumigatus with the bacteria Streptomyces peucetius led to the induction of production of formyl xanthocillin analogues. This mixed fermentation yielded two new metabolites, fumiformamide (1) and N,N'-((1Z,3Z)-1,4-bis(4-methoxyphenyl)buta-1,3-diene-2,3-diyl)diformamide (2), together with two known N-formyl derivatives and the xanthocillin analogue BU-4704. The structures were determined by spectroscopic methods and by comparison with literature. Cytotoxic activity of all the analogues was tested on the NCI-60 cell line screen, and compound 2 exhibited significant activity against several cell lines. The analogues did not show antimicrobial activity.
authors.affiliation: SAIC-Frederick, Inc., P.O. Box B, Frederick, Maryland 21702, USA. zuckk@mail.nih.gov
authors.forename: Karina M
authors.initials: KM
authors.lastname: Zuck
authors.forename: Suzanne
authors.initials: S
authors.lastname: Shipley
authors.forename: David J
authors.initials: DJ
authors.lastname: Newman
mesh_terms: D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D001232:Aspergillus fumigatus / Q000378:metabolism*; D002070:Butadienes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D018920:Coculture Techniques; D004354:Drug Screening Assays, Antitumor; D005285:Fermentation; D005559:Formamides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D009570:Nitriles / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D019906:Nuclear Magnetic Resonance, Biomolecular; D013302:Streptomyces / Q000378:metabolism*
chemical_list: D000470:Alkaloids; D000970:Antineoplastic Agents; C081423:BU 4704; D002070:Butadienes; D005559:Formamides; C561561:N,N'-((1Z,3Z)-1,4-bis(4-methoxyphenyl)buta-1,3-diene-2,3-diyl)diformamide; D009570:Nitriles; C561562:fumiformamide