title: Investigation of the antigenic evolution of field isolates using the reverse genetics system of infectious bursal disease virus (IBDV).
abstract: The antigenic profiles of over 300 infectious bursal disease virus (IBDV) isolates were analyzed using a panel of monoclonal antibodies in a reverse genetics system. In addition, the sequences of a large portion of the neutralizing-antibody-inducing VP2 of IBDV were determined. Phylogenetic analysis of nucleotide and amino acid sequences in combination with the antigenic profiles obtained using the monoclonal antibody panel, revealed a lack of correlation between antigenicity and isolate's placement within the phylogenetic tree. In-depth analysis of amino acid exchanges revealed that changes within a certain region of the VP2 molecule resulted in differences in the antigenicity of the virus. This comprehensive analysis of VP2 sequences indicated a high selective pressure in the field that was likely due to vaccination programs, which increase the rate of evolution of the virus.
authors.affiliation: Poultry Diagnostic and Research Center, College of Veterinary Medicine, University of Georgia, Athens, GA, USA.
authors.forename: Vijay
authors.initials: V
authors.lastname: Durairaj
authors.forename: Holly S
authors.initials: HS
authors.lastname: Sellers
authors.forename: Erich G
authors.initials: EG
authors.lastname: Linnemann
authors.forename: Alan H
authors.initials: AH
authors.lastname: Icard
authors.forename: Egbert
authors.initials: E
authors.lastname: Mundt
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D005075:Biological Evolution; D018175:Birnaviridae Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D002645:Chickens; D007243:Infectious bursal disease virus / Q000145:classification / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D011201:Poultry Diseases / Q000276:immunology / Q000821:virology*; D016415:Sequence Alignment; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology
chemical_list: D000956:Antigens, Viral; D014764:Viral Proteins