title: Isoptericola nanjingensis sp. nov., a mineral-weathering bacterium.
abstract: A Gram-positive-staining, non-motile, rod- or coccoid-shaped actinobacterium, designated strain H17T, was isolated from a soil sample from Nanjing, Jiangsu Province, PR China. The organism grew optimally at 30 °C, pH 7.0 and with 3 % NaCl (w/v). Strain H17T contained L-Lys-D-Asp as the cell-wall peptidoglycan type and galactose, xylose and mannose as the whole-cell sugars. The major fatty acids were anteiso-C15:0 and iso-C15:0. The total polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phospholipids, phosphatidylinositol, phosphoglycolipid, one unidentified glycolipid and one unidentified lipid. The menaquinone was MK9(H4). Mycolic acids were not detected. The DNA G+C content was 72.4 mol%. Phylogenetic analysis of this strain based on 16S rRNA gene sequences revealed 97.8-99.6 % similarity to recognized species of the genus Isoptericola. The low level of DNA-DNA relatedness to other species of the genus Isoptericola and the many phenotypic properties that distinguished strain H17T from recognized species of this genus demonstrated that isolate H17T should be classified as representing a novel species of the genus Isoptericola. The name Isoptericola nanjingensis sp. nov. is proposed for this novel species. The type strain is H17T (=DSM 24300T=CCTCC AB 2011005T).
authors.affiliation: Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Xia-Fang
authors.initials: XF
authors.lastname: Sheng
authors.affiliation: Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Fei
authors.initials: F
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Lin-Yan
authors.initials: LY
authors.lastname: He
authors.affiliation: Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Huang
authors.affiliation: Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C073355:menaquinone 9