title: Direct monitoring changes of salbutamol concentration in serum by chemiluminescent imaging.
abstract: We report in this manuscript, the use of direct ammonium persulfate-enhanced chemiluminescence (CL) imaging, to monitor changes to measure serum salbutamol concentration in subjects of different haptoglobin (Hp) phenotypes at different dosing time. It was noted that CL generated from Hp was decreased due to salbutamol's reducibility, which was used for monitoring salbutamol concentration in serum. The serum from the subjects treated by oral administration of salbutamol, was collected at different dosing time and was separated by polyacrylamide gel electrophoresis (PAGE) prior to the CL detection. According to CL images, samples were separated into three groups based on the Hp phenotypes. The curves of CL signal intensity versus time were obtained for each group, and we demonstrated that there were more significant variables on binding ability between groups. The maximum salbutamol concentration in the serum appeared after 4h, which was in agreement with the literature. In addition, the binding constants of salbutamol to Hp were determined by a fluorescence-based method, whose results were in agreement with the phenomenon of the greater salbutamol metabolism rate for Group Hp 1-1 than Group Hp 2-2. The presented method can monitor changes of salbutamol concentration in serum directly, making the procedures much simple, convenient, rapid and has the property of lower cost. It provided us with excellent reference information for the individual dosage regimen of different Hp groups, which hopefully could become a potential method for further pharmaceutical research.
authors.affiliation: College of Chemistry, Beijing Normal University, Beijing, PR China.
authors.forename: Canli
authors.initials: C
authors.lastname: Zhang
authors.forename: Ruichao
authors.initials: R
authors.lastname: Zhang
authors.forename: Na
authors.initials: N
authors.lastname: Na
authors.forename: Joris R
authors.initials: JR
authors.lastname: Delanghe
authors.forename: Jin
authors.initials: J
authors.lastname: Ouyang
mesh_terms: D000284:Administration, Oral; D000328:Adult; D000420:Albuterol / Q000008:administration & dosage / Q000097:blood* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006242:Haptoglobins / Q000378:metabolism; D006801:Humans; D016014:Linear Models; D008163:Luminescent Measurements / Q000379:methods*; D008297:Male; D010641:Phenotype; D011485:Protein Binding; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013993:Tilidine / Q000008:administration & dosage / Q000097:blood
chemical_list: D006242:Haptoglobins; D013993:Tilidine; D000420:Albuterol