title: Brush-like copolymer as a physically adsorbed coating for protein separation by capillary electrophoresis.
abstract: A brush-like copolymer consisting of poly(ethylene glycol) methyl ether methacrylate and N,N-dimethylacrylamide (PEGMA-DMA) was synthesized and used as a novel static physically adsorbed coating for protein separation by capillary electrophoresis for the first time, in order to stabilize electroosmotic flow (EOF) and suppress adsorption of proteins onto the capillary wall. Very stable and low EOF was obtained in PEGMA-DMA-coated capillary at pH 2.2-7.8. The effects of molar ratio of PEGMA to DMA, copolymer molecular mass, and pH on the separation of basic proteins were discussed. A comparative study of bare capillary with PEGMA-DMA-coated capillary for protein separation was also performed. The basic proteins could be well separated in PEGMA-DMA-coated capillary over the investigated pH range of 2.8-6.8 with good repeatability and high separation efficiency because the copolymer coating combines good protein-resistant property of PEG side chains with excellent coating ability of PDMA-contained backbone. Finally, the coating was successfully applied to the fast separation of other protein samples, such as protein mixture and egg white, which reveals that it is a potential coating for further proteomics analysis.
authors.affiliation: CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei, PR China.
authors.forename: Dan
authors.initials: D
authors.lastname: Zhou
authors.forename: Lin
authors.initials: L
authors.lastname: Tan
authors.forename: Lina
authors.initials: L
authors.lastname: Xiang
authors.forename: Rongju
authors.initials: R
authors.lastname: Zeng
authors.forename: Fuhu
authors.initials: F
authors.lastname: Cao
authors.forename: Xiaoxi
authors.initials: X
authors.lastname: Zhu
authors.forename: Yanmei
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000327:Adsorption; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D011108:Polymers; D011506:Proteins / Q000032:analysis / Q000302:isolation & purification*
chemical_list: D011108:Polymers; D011506:Proteins