title: A survey of the cellular responses in Pseudomonas putida KT2440 growing in sterilized soil by microarray analysis.
abstract: Genome-wide scanning of gene expression by microarray techniques was successfully performed on RNA extracted from sterilized soil inoculated with Pseudomonas putida KT2440/pSL1, which contains a chloroaromatic degrading plasmid, in the presence or absence of 3-chlorobenzoic acid (3CB). The genes showing significant changes in their expression in both the triplicate-microarray analysis using amplified RNA and the single-microarray analysis using unamplified RNA were investigated. Pathway analysis revealed that the benzoate degradation pathway underwent the most significant changes following treatment with 3CB. Analysis based on categorization of differentially expressed genes against 3CB revealed new findings about the cellular responses of the bacteria to 3CB. Genes specifically involved in the transport of 3CB were upregulated, including a K(+)/H(+) antiporter complex, a universal stress protein, two cytochrome P450 proteins and an efflux transporter. The downregulated expression of several genes involved in carbon metabolism and the genes belonging to a prophage in the presence of 3CB was observed. This study demonstrated the applicability of the method of soil RNA extraction for microarray analysis of gene expression in bacteria growing in sterilized soil.
authors.affiliation: Environmental Biofunction Division, National Institute for Agro-Environmental Sciences, Tsukuba, Ibaraki, Japan.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.forename: Sho
authors.initials: S
authors.lastname: Morimoto
authors.forename: Naoto
authors.initials: N
authors.lastname: Ogawa
authors.forename: Takeshi
authors.initials: T
authors.lastname: Fujii
mesh_terms: D002723:Chlorobenzoates / Q000378:metabolism / Q000633:toxicity*; D015870:Gene Expression; D005798:Genes, Bacterial; D046228:Microarray Analysis; D008969:Molecular Sequence Data; D010957:Plasmids; D016958:Pseudomonas putida / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D012313:RNA / Q000235:genetics / Q000378:metabolism; D012987:Soil; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism / Q000633:toxicity*
chemical_list: D002723:Chlorobenzoates; D012987:Soil; D012989:Soil Pollutants; C036427:3-chlorobenzoic acid; D012313:RNA