title: A genetic algorithm for the optimization of the thermoinduction protocol for high-level production of recombinant human-like collagen from Escherichia coli.
abstract: Production of recombinant human-like collagen (RHLC) by thermoinduction of recombinant Escherichia coli BL 21 during high cell density cultivation was investigated in a 30 L bioreactor. The effects of induction temperature (T), pH, and carbon-to-nitrogen molar ratio of the nutrient medium (C/N) were examined. The optimal thermoinduction protocol for RHLC production was determined by using a model coupling genetic algorithm and artificial neural networks. The optimal operating conditions were as follows: maintenance of induction temperature at 42°C for 3 H and then at 39.4°C until the end, induction pH at 7.03, and C/N at 4.8 (mol/mol). The theoretical maximum concentration of RHLC was 12.5 g/L, whereas the experimental value was 12.1 g/L under the optimal induction conditions.
authors.affiliation: Shaanxi R&D Center of Biomaterial and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Chi
authors.forename: Dai-Di
authors.initials: DD
authors.lastname: Fan
authors.forename: Xiao-Xuan
authors.initials: XX
authors.lastname: Ma
authors.forename: Yu
authors.initials: Y
authors.lastname: Mi
authors.forename: Yan-E
authors.initials: YE
authors.lastname: Luo
authors.forename: Chen-Hui
authors.initials: CH
authors.lastname: Zhu
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Zhu
authors.forename: Wen-Jiao
authors.initials: WJ
authors.lastname: Xue
mesh_terms: D000465:Algorithms; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D003094:Collagen / Q000096:biosynthesis* / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D013696:Temperature
chemical_list: D011994:Recombinant Proteins; D003094:Collagen