title: Simiduia areninigrae sp. nov., an agarolytic bacterium isolated from sea sand.
abstract: During a study intended to screen for agar-degrading bacteria, strain M2-5T was isolated from black sand off the shore of Jeju Island, Republic of Korea. Strain M2-5T exhibited agarase activity; the β-agarase gene of the isolate had 62 % amino acid sequence identity to the β-agarase gene of Microbulbifer thermotolerans JAMB A94T. The isolate was closely related to members of the genus Simiduia but was clearly discernible from reported Simiduia species, based on a polyphasic analysis. Cells of strain M2-5T were Gram-negative, catalase- and oxidase-positive, motile rods. The DNA G+C content was 53.3 mol%. The predominant isoprenoid quinone was Q-8. The major cellular fatty acids were C17:1ω8c (25.9 %), summed feature 3 (iso-C(15 : 0) 2-OH and/or C16:1ω7c; 17.2 %) and C17:0 (15.0 %). Phylogenetic analysis using 16S rRNA gene sequences showed that strain M2-5T had 96.6 % gene sequence similarity to Simiduia agarivorans SA1T, the most closely related type strain of the genus Simiduia. These results suggest that strain M2-5T represents a novel species in the genus Simiduia, for which the name Simiduia areninigrae sp. nov. is proposed; the type strain is M2-5T (=KCTC 23293T=NCAIM B 02424T).
authors.affiliation: Microbiological Resources Center, KRIBB, Daejeon 305-806, Republic of Korea.
authors.forename: Byung-Chun
authors.initials: BC
authors.lastname: Kim
authors.affiliation: Bionanotechnology Research Center, KRIBB, Daejeon 305-806, Republic of Korea.
authors.forename: Haryoung
authors.initials: H
authors.lastname: Poo
authors.affiliation: Microbiological Resources Center, KRIBB, Daejeon 305-806, Republic of Korea.
authors.forename: Kang Hyun
authors.initials: KH
authors.lastname: Lee
authors.affiliation: Microbiological Resources Center, KRIBB, Daejeon 305-806, Republic of Korea.
authors.forename: Mi Na
authors.initials: MN
authors.lastname: Kim
authors.affiliation: Microbiological Resources Center, KRIBB, Daejeon 305-806, Republic of Korea.
authors.forename: Doo-Sang
authors.initials: DS
authors.lastname: Park
authors.affiliation: Industrial Biomaterials Research Center, KRIBB Daejeon 305-806, Republic of Korea.
authors.forename: Hyun Woo
authors.initials: HW
authors.lastname: Oh
authors.affiliation: Department of Food and Biotechnology, Hoseo University, Asan 336-795, Republic of Korea.
authors.forename: Jin Man
authors.initials: JM
authors.lastname: Lee
authors.affiliation: Microbiological Resources Center, KRIBB, Daejeon 305-806, Republic of Korea.
authors.forename: Kee-Sun
authors.initials: KS
authors.lastname: Shin
mesh_terms: D000362:Agar / Q000378:metabolism; D034241:Alteromonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005798:Genes, Bacterial; D006026:Glycoside Hydrolases / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012822:Silicon Dioxide; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; D012822:Silicon Dioxide; D000362:Agar; C030778:ubiquinone 8; D006026:Glycoside Hydrolases; C019624:agarase