title: Increased expression of cellular FLICE/caspase-8 inhibitory protein in condyloma acuminatum.
abstract: Condyloma acuminatum (CA) is a benign epithelial tumor caused by infection with human papillomaviruses (HPVs) and characterized by abnormal cell proliferation. Cellular caspase-8 (FLICE)-like inhibitory protein (c-FLIP) was originally identified as an inhibitor of death-receptor signaling through competition with caspase-8 for recruitment to FAS-associated via death domain (FADD). More recently, it has been determined that c-FLIP is associated with the survival and proliferation of T cells and keratinocytes. The aim of this work was to study the expression of c-FLIP in CA and its relationship with keratinocyte proliferation. Immunoperoxidase staining methods were applied to analyze the location and expressions of both c-FLIP and proliferating cell nuclear antigens (PCNA) in 34 CA and 16 normal foreskin tissues. Semiquantitative reverse transcriptase-polymerase chain reactions (RT-PCR) and western blotting were performed to further identify the expression of c-FLIP in CA. c-FLIP expression at both mRNA and protein level was significantly higher in CA than normal foreskin. c-FLIP expression was highly correlated with the PCNA labeling index (LI) in CA. We concluded that c-FLIP overexpression might take part in keratinocyte proliferation in CA.
authors.affiliation: Department of Dermatology, Union Hospital, Tongji Medical College, Huazhong University, Wuhan Hubei 430022, PR China.
authors.forename: Fen
authors.initials: F
authors.lastname: Tian
authors.forename: Jiejie
authors.initials: J
authors.lastname: Lu
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Guanxin
authors.initials: G
authors.lastname: Shen
authors.forename: Yating
authors.initials: Y
authors.lastname: Tu
authors.forename: Juan
authors.initials: J
authors.lastname: Tao
mesh_terms: D000328:Adult; D015153:Blotting, Western; D053446:CASP8 and FADD-Like Apoptosis Regulating Protein / Q000378:metabolism*; D049109:Cell Proliferation; D003218:Condylomata Acuminata / Q000378:metabolism*; D052816:Foreskin / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D015603:Keratinocytes / Q000378:metabolism*; D008297:Male; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018709:Statistics, Nonparametric
chemical_list: D053446:CASP8 and FADD-Like Apoptosis Regulating Protein