title: Actinoplanes abujensis sp. nov., isolated from Nigerian arid soil.
abstract: A novel actinobacterial strain, A4029T, isolated from arid soil of Abuja, Nigeria, and provisionally assigned to the genus Actinoplanes, was subjected to a polyphasic taxonomic study. 16S rRNA gene sequence similarity studies showed that strain A4029T belonged to the genus Actinoplanes, being most closely related to Actinoplanes brasiliensis DSM 43805T (98.9 %) and Actinoplanes deccanensis DSM 43806T (98.0 %); similarity to other type strains of the genus Actinoplanes ranged from 96.2 to 97.9 %. Chemotaxonomic data [major menaquinone MK-9(H4); major polar lipids phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol; characteristic sugars arabinose and xylose; major fatty acids iso-C15:0, anteiso-C15:0, iso-C16:0, C17:1ω9c and iso-C14:0] confirmed the affiliation of strain A4029T to the genus Actinoplanes. The results of DNA-DNA hybridizations and phylogenetic analysis, together with phenotypic and biochemical test data, allowed strain A4029T to be differentiated from strains of other Actinoplanes species. Therefore, strain A4029T represents a novel species, for which the name Actinoplanes abujensis sp. nov. is proposed, with A4029T (=DSM 45518T=NRRL B-24835T=KCTC 19984T) as the type strain.
authors.affiliation: Department of Biology, Faculty of Art and Science, Ondokuz Mayıs University, 55139 Kurupelit-Samsun, Turkey.
authors.forename: Anil
authors.initials: A
authors.lastname: Sazak
authors.affiliation: Department of Biology, Faculty of Art and Science, Ondokuz Mayıs University, 55139 Kurupelit-Samsun, Turkey.
authors.forename: Nevzat
authors.initials: N
authors.lastname: Sahin
authors.affiliation: Department of Biology, Faculty of Art and Science, Ondokuz Mayıs University, 55139 Kurupelit-Samsun, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Camas
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008848:Micromonosporaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009549:Nigeria; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D010858:Pigmentation; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C073355:menaquinone 9