title: [Evaluation of vitro hepatotoxicity of monocrotaline by precision-cut liver slice technique].
abstract: OBJECTIVE
  To modify the empirical method of precision-cut liver slice technique, and study the hepatotoxicity of monocrotaline by this technique.
  
  
  METHODS
  Liver slices were prepared by the domestic shaking slicer. The technique of precision-cut liver slice was established by detecting MTT reduction used as the slice viability under different culture medium, thickness of slices, pH and culture temperature. After monocrotaline and liver slices co-culture for 6, 24 h, the slice viability, enzyme activity of GPT, GOT, LDH, GGT and protein concentration were detected by MTT reduction, enzyme kinetics method and BCA protein assay method, respectively.
  
  
  RESULTS
  When the thickness of slices was 200 microm and pH of medium was 6.8, culture temperature was 37 degrees C, BPM culture medium, the viability of slices could maintain on a steady level. LDH leakage was significantly increased and protein content was obviously decreased after monocrotaline co-culture for 24 h with final concentration 0.02, 0.1 and 0.5 g x L(-1). No statistically significant difference between control group and monocrotaline 3 dose groups was observed in the slice viability and the content of GPT, GOT, LDH, GGT and protein after monocrotaline co-culture for 6 h.
  
  
  CONCLUSIONS
  The slice viability could retain 24 h in modified BPM medium surroundings; monocrotaline displayed liver toxicity in some degree after co-culture for 24 hours in 0.02, 0.1 and 0.5 g x L(-1) concentration.
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
authors.forename: Lianqiang
authors.initials: L
authors.lastname: Hui
authors.forename: Shuangrong
authors.initials: S
authors.lastname: Gao
authors.forename: Ting
authors.initials: T
authors.lastname: Liu
authors.forename: Chunyu
authors.initials: C
authors.lastname: Cao
authors.forename: Jing
authors.initials: J
authors.lastname: Guo
authors.forename: Ran
authors.initials: R
authors.lastname: Hao
authors.forename: Yan
authors.initials: Y
authors.lastname: Yi
authors.forename: Chunying
authors.initials: C
authors.lastname: Li
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhao
authors.forename: Aihua
authors.initials: A
authors.lastname: Liang
mesh_terms: D000818:Animals; D006863:Hydrogen-Ion Concentration; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D016686:Monocrotaline / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013696:Temperature; D018675:Toxicity Tests
chemical_list: D016686:Monocrotaline; D007770:L-Lactate Dehydrogenase