title: Trials of the detection of semen and vaginal fluid RNA using the genome profiling method.
abstract: The identification of sperm at the scene of a sexual crime is important evidence that can be used to prove that a crime took place. We used the new genome profiling (GP) method in this study to identify sperm and vaginal fluid from RNA extracted from bodily fluids. We randomly amplified genes via a PCR approach from these semen and vaginal fluid samples and performed temperature gradient gel electrophoresis between 15-65°C. We identified specific species identification dots (spiddos) for semen and vaginal fluid. The results showed that the GP method is effective for the identification of bodily fluids at the scene of a sex crime.
authors.affiliation: Department of Forensic Medicine, Kyoto Prefectural University of Medicine Graduate School of Medical Sciences, Kajii-cho, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto, Japan.
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Takasaka
authors.forename: Koichi
authors.initials: K
authors.lastname: Sakurada
authors.forename: Tomoko
authors.initials: T
authors.lastname: Akutsu
authors.forename: Koichi
authors.initials: K
authors.lastname: Nishigaki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ikegaya
mesh_terms: D000328:Adult; D016172:DNA Fingerprinting; D005260:Female; D053803:Forensic Genetics; D006801:Humans; D007564:Japan; D008297:Male; D008875:Middle Aged; D012313:RNA; D011902:Rape / Q000175:diagnosis*; D012661:Semen; D014626:Vaginal Smears
chemical_list: D012313:RNA