title: Preparation and atomic force microscopy of quadruplex DNA.
abstract: The purpose of this chapter is to provide detailed instructions for the preparation and atomic force microscopy (AFM) imaging of linear chains of quadruplex DNA (a.k.a. "G-wire DNA"). Successful self-assembly of long chain quadruplex DNA requires pure concentrated guanine-rich oligonucleotide sequence (GROs) and monovalent cations in a growth buffer. AFM imaging of individual G-wire DNA strands requires many carefully monitored steps, including substrate preparation, G-wire concentration, adsorption onto substrate, rinsing, drying, appropriate selection/use of imaging probes, and dry atmosphere imaging conditions. Detailed step-wise instructions are provided.
authors.affiliation: Department of Chemistry and Physics, University of New England, Biddeford, ME, USA. jvesenka@une.edu
authors.forename: James
authors.initials: J
authors.lastname: Vesenka
mesh_terms: D001482:Base Composition; D001483:Base Sequence; D004247:DNA / Q000138:chemical synthesis* / Q000648:ultrastructure; D054856:G-Quadruplexes; D018625:Microscopy, Atomic Force / Q000379:methods*; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000138:chemical synthesis; D019098:Telomerase; D013696:Temperature; D016808:Tetrahymena thermophila / Q000235:genetics
chemical_list: D009841:Oligonucleotides; D004247:DNA; D019098:Telomerase