title: Vaccination of goats with a glutathione peroxidase DNA vaccine induced partial protection against Haemonchus contortus infection.
abstract: Due to their critical functions in worm physiology, glutathione peroxidases in Haemonchus contortus are potential candidates for vaccine to control haemonchosis. However, information on the protection potential of these molecules is lacking. In this study, recombinant HC29 antigen was tested for protection against experimental H. contortus infections in goats. Fifteen animals were allocated into three trial groups. The animals of HC29 group was vaccinated with a recombinant HC29 DNA vaccine twice at day 0 and day 14, and then challenged with 5000 infective H. contortus L3 (third larval stage) on the 28th day. An unvaccinated positive control group was challenged with L3 at the same time. An unvaccinated negative control group was not challenged with L3. The results indicated HC29 DNA vaccine was transcribed at local injection sites and expressed in vivo post immunizations, respectively. Following L3 challenge, the mean eggs per gram feces and worm burdens of HC29 group were reduced by 36.1% and 35.6% respectively compared to the positive control group. After immunization with the DNA vaccine, significantly high levels of serum IgG, serum IgA, mucosal IgA and CD4(+) T lymphocytes were detected. These results suggest that recombinant H. contortus HC29 glutathione peroxidase DNA vaccine induced a partial immune response and has protective potentials against caprine haemonchosis.
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, Jiangsu, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
authors.forename: Xiaokai
authors.initials: X
authors.lastname: Song
authors.forename: Ruofeng
authors.initials: R
authors.lastname: Yan
authors.forename: Lixin
authors.initials: L
authors.lastname: Xu
authors.forename: Xiangrui
authors.initials: X
authors.lastname: Li
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D016207:Cytokines / Q000097:blood; D005786:Gene Expression Regulation; D005979:Glutathione Peroxidase / Q000235:genetics* / Q000276:immunology*; D015511:Goat Diseases / Q000517:prevention & control*; D006041:Goats; D006188:Haemonchiasis / Q000517:prevention & control / Q000662:veterinary*; D006189:Haemonchus / Q000276:immunology*; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D016131:Lymphocyte Subsets; D013997:Time Factors; D019444:Vaccines, DNA / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth; D016207:Cytokines; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D019444:Vaccines, DNA; D005979:Glutathione Peroxidase