title: A 4-week repeated oral dose toxicity study of dairy fat naturally enriched in vaccenic, rumenic and α-linolenic acids in rats.
abstract: Few studies have focused on the toxicological risks of dairy fat intake. A standard dairy fat (SDF) with a 70% SFA content and a naturally enriched dairy fat (EDF) in vaccenic, rumenic and α-linolenic acids and low in SFA (54%) have been examined in a 4-week repeated dose oral toxicity study as a daily dose of 2000 mg/kg bw by gavage in rats. Comparisons were established with a third group of rats (control) which did not receive fat administration. Both fats were well tolerated, and no adverse events or mortality were observed during the treatment nor after a 2-week observation period. EDF and SDF did not cause significant differences with respect to a control group in body weight gain, food consumption, clinical observations, organ weight ratios, histopathological findings and most of the hematological and biochemical parameters including total cholesterol and cholesterol fractions in plasma. In rats treated with SDF, a significant increase of triglycerides was observed as compared to the control group. By contrast, in rats treated with EDF, a significant decrease in triglycerides was detected. EDF orally administered to rats was safe, and no treatment-related toxicity was detected. The results also suggest that EDF could protect against the increase of triglyceride concentrations in plasma.
authors.affiliation: Department of Toxicology and Pharmacology, Universidad Complutense de Madrid , Madrid, Spain.
authors.forename: Arturo
authors.initials: A
authors.lastname: Anadon
authors.forename: Maria Rosa
authors.initials: MR
authors.lastname: Martinez-Larranaga
authors.forename: Maria Aranzazu
authors.initials: MA
authors.lastname: Martinez
authors.forename: Irma
authors.initials: I
authors.lastname: Ares
authors.forename: Eva
authors.initials: E
authors.lastname: Ramos
authors.forename: Pilar
authors.initials: P
authors.lastname: Gomez-Cortes
authors.forename: Manuela
authors.initials: M
authors.lastname: Juarez
authors.forename: Miguel Angel
authors.initials: MA
authors.lastname: de la Fuente
mesh_terms: D000818:Animals; D003611:Dairy Products / Q000009:adverse effects* / Q000032:analysis; D004041:Dietary Fats / Q000008:administration & dosage / Q000009:adverse effects* / Q000032:analysis / Q000378:metabolism; D005260:Female; D006041:Goats; D044243:Linoleic Acids, Conjugated / Q000008:administration & dosage / Q000009:adverse effects* / Q000032:analysis / Q000378:metabolism; D008297:Male; D009829:Oleic Acids / Q000008:administration & dosage / Q000009:adverse effects* / Q000032:analysis / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014280:Triglycerides / Q000097:blood; D017962:alpha-Linolenic Acid / Q000008:administration & dosage / Q000009:adverse effects* / Q000032:analysis / Q000378:metabolism
chemical_list: D004041:Dietary Fats; D044243:Linoleic Acids, Conjugated; D009829:Oleic Acids; D014280:Triglycerides; D017962:alpha-Linolenic Acid; C046938:9,11-linoleic acid; C050413:11-octadecenoic acid