title: Direct synthesis of Rev peptide-conjugated gold nanoparticles and their application in cancer therapeutics.
abstract: We have developed a simple approach for generating peptide-conjugated gold nanoparticles (AuNPs) from the Rev peptide and gold aqueous solution. The peptide functions as both a reducing agent and a capping molecule. AuNPs of various sizes (20-300 nm) and shapes (spheres, triangular plates, and polygons) can be obtained upon modulating the ratio of gold ions to the Rev peptide. Transmission electron microscopy, X-ray diffraction, and UV-vis spectroscopy were utilized to characterize these nanoparticles. Fourier-transform infrared and X-ray photoelectron spectroscopy measurements were performed to investigate chemical interactions between the Rev peptide and AuNPs. Lactate dehydrogenase and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays revealed that the Rev peptide-AuNP nanocomposites exhibited exceptionally high cytotoxic effects toward mouse ovarian surface epithelial cell lines, relative to the effects of equal doses of the free Rev peptide. Our study suggests a new way of utilizing biomolecule-conjugated AuNPs as potentially effective anticancer drugs.
authors.affiliation: Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei, Taiwan.
authors.forename: Ngoc Thi Thanh
authors.initials: NT
authors.lastname: Tran
authors.forename: Tzu-Hao
authors.initials: TH
authors.lastname: Wang
authors.forename: Chiao-Yun
authors.initials: CY
authors.lastname: Lin
authors.forename: Yi-Chun
authors.initials: YC
authors.lastname: Tsai
authors.forename: Chyong-Huey
authors.initials: CH
authors.lastname: Lai
authors.forename: Yian
authors.initials: Y
authors.lastname: Tai
authors.forename: Benjamin Y M
authors.initials: BY
authors.lastname: Yung
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006046:Gold / Q000737:chemistry* / Q000494:pharmacology; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009369:Neoplasms / Q000188:drug therapy*; D010316:Particle Size; D010455:Peptides / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D000970:Antineoplastic Agents; D010455:Peptides; C503402:Rev peptide; D006046:Gold