title: Contribution of laboratory methods in diagnosing clinically suspected ocular toxoplasmosis in Brazilian patients.
abstract: This prospective study evaluated the value of laboratorial diagnosis in ocular toxoplasmosis analyzing peripheral blood samples from a group of Brazilian patients by immunologic and molecular methods. We analyzed blood samples from 184 immunocompetent patients with ocular disorders divided into 2 groups: Group I, composed of samples from 49 patients with ocular toxoplasmosis diagnosed by clinical features; Group II, samples from 135 patients with other ocular diseases. Samples were assayed by conventional polymerase chain reaction (cnPCR), real-time PCR (qPCR) for Toxoplasma gondii, indirect immunofluorescence reaction (IF), avidity test (crude tachyzoite lysate as antigen), and excreted-secreted tachyzoite proteins as antigen (ESA-ELISA). cnPCR and qPCR profiles were concordant in all samples. Positive PCR was shown in 40.8% of group I patients. The majority of the positive blood samples (75%) were taken from patients with toxoplasmic retinochoroiditis scars, and the others (25%), from patients with retinal exudative lesions. Despite that 86 of the 135 patients from Group II had asymptomatic toxoplasmosis, all DNA blood samples had negative PCR. Concordant results were shown in the data obtained by serologic methods. Around 24% of the patients with ocular toxoplasmosis had high antibody titers determined by ESA-ELISA and IF. Anti-ESA antibodies are shown principally in patients with active infection. Collectively, these data demonstrate the presence of tachyzoites in the blood of patients with chronic infection, supporting the idea of recurrent disease. Circulating parasites in blood of immunocompetent individuals may be associated with the reactivation of the ocular disease.
authors.affiliation: Laboratório de Imunogenética, Departamento de Biologia Molecular, Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto, SP, Brazil.
authors.forename: Cinara C B
authors.initials: CC
authors.lastname: Mattos
authors.forename: Cristina S
authors.initials: CS
authors.lastname: Meira
authors.forename: Ana I C
authors.initials: AI
authors.lastname: Ferreira
authors.forename: Fábio B
authors.initials: FB
authors.lastname: Frederico
authors.forename: Roberto M
authors.initials: RM
authors.lastname: Hiramoto
authors.forename: Gildásio C
authors.initials: GD
authors.lastname: Almeida
authors.forename: Luiz C
authors.initials: LC
authors.lastname: Mattos
authors.forename: Vera L
authors.initials: VL
authors.lastname: Pereira-Chioccola
mesh_terms: D000913:Antibodies, Protozoan / Q000032:analysis; D001769:Blood / Q000469:parasitology; D001938:Brazil; D019411:Clinical Laboratory Techniques / Q000379:methods*; D016054:DNA, Protozoan / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D007118:Immunoassay / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods; D011446:Prospective Studies; D014122:Toxoplasma / Q000302:isolation & purification*; D014126:Toxoplasmosis, Ocular / Q000175:diagnosis*
chemical_list: D000913:Antibodies, Protozoan; D016054:DNA, Protozoan