title: Soft matrix supports osteogenic differentiation of human dental follicle cells.
abstract: The differentiation of stem cells can be directed by the grade of stiffness of the developed tissue cells. For example a rigid extracellular matrix supports the osteogenic differentiation in bone marrow derived mesenchymal stem cells (MSCs). However, less is known about the relation of extracellular matrix stiffness and cell differentiation of ectomesenchymal dental precursor cells. Our study examined for the first time the influence of the surface stiffness on the proliferation and osteogenic differentiation of human dental follicle cells (DFCs). Cell proliferation of DFCs was only slightly decreased on cell culture surfaces with a bone-like stiffness. The osteogenic differentiation in DFCs could only be initiated with a dexamethasone based differentiation medium after using varying stiffness. Here, the softest surface improved the induction of osteogenic differentiation in comparison to that with the highest stiffness. In conclusion, different to bone marrow derived MSCs, soft ECMs have a superior capacity to support the osteogenic differentiation of DFCs.
authors.affiliation: Department of Operative Dentistry and Periodontology, University Hospital Regensburg, Regensburg, Germany.
authors.forename: Sandra
authors.initials: S
authors.lastname: Viale-Bouroncle
authors.forename: Florian
authors.initials: F
authors.lastname: Völlner
authors.forename: Christoph
authors.initials: C
authors.lastname: Möhl
authors.forename: Kevin
authors.initials: K
authors.lastname: Küpper
authors.forename: Gero
authors.initials: G
authors.lastname: Brockhoff
authors.forename: Torsten E
authors.initials: TE
authors.lastname: Reichert
authors.forename: Gottfried
authors.initials: G
authors.lastname: Schmalz
authors.forename: Christian
authors.initials: C
authors.lastname: Morsczeck
mesh_terms: D018929:Cell Culture Techniques; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D003795:Dental Sac / Q000166:cytology* / Q000187:drug effects; D003907:Dexamethasone / Q000494:pharmacology; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D010012:Osteogenesis; D013499:Surface Properties; D055815:Young Adult
chemical_list: D003470:Culture Media; D003907:Dexamethasone