title: Chemical functionalization of polysilicon microparticles for single-cell studies.
abstract: In this work, two types of polycrystalline silicon (polysilicon) microparticles were modified with specific ligands in order to be selectively attached to chemical residues located at the plasma membrane and thus to be applied to study individual cells in culture. Two different functionalization approaches based on adsorption and covalent attachment were assayed. A comparative study of the efficiency of the ligand immobilization and stability of the modified particle in the culture medium was carried out using the selected ligands labeled with a fluorophore. Cylindrical microparticles (nonencoded microparticles) and shape-encoded microparticles (bar codes) were used with the aim of demonstrating the nondependence of the particle size and shape on the efficiency of the immobilization protocol. Fluorescence imaging and statistical analysis of the recorded fluorescence intensity showed that the covalent attachment of the ligand to the surface of the microparticle, previously modified with an aldehyde-terminated silane, gave the best results. As a proof of concept, Vero cells in culture were labeled with the covalently modified bar codes and successfully tracked for up to 1 week without observing any alteration in the viability of the cells. Bar code numbers could be easily read by eye using a bright-field optical microscope. It is anticipated that such modified microparticles could be feasible platforms for the introduction of other analytical functions of interest in single-cell monitoring and cell sorting in automatic analysis systems.
authors.affiliation: Instituto de Microelectrónica de Barcelona, IMB-CNM (CSIC), Campus UAB, 08193-Bellaterra, Barcelona, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Fernández-Rosas
authors.forename: A
authors.initials: A
authors.lastname: Baldi
authors.forename: E
authors.initials: E
authors.lastname: Ibañez
authors.forename: L
authors.initials: L
authors.lastname: Barrios
authors.forename: S
authors.initials: S
authors.lastname: Novo
authors.forename: J
authors.initials: J
authors.lastname: Esteve
authors.forename: J A
authors.initials: JA
authors.lastname: Plaza
authors.forename: M
authors.initials: M
authors.lastname: Duch
authors.forename: R
authors.initials: R
authors.lastname: Gómez
authors.forename: O
authors.initials: O
authors.lastname: Castell
authors.forename: C
authors.initials: C
authors.lastname: Nogués
authors.forename: C
authors.initials: C
authors.lastname: Fernández-Sánchez
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000737:chemistry; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D008024:Ligands; D015394:Molecular Structure; D010316:Particle Size; D011108:Polymers / Q000737:chemistry*; D012825:Silicon / Q000737:chemistry*; D013499:Surface Properties; D014709:Vero Cells
chemical_list: D008024:Ligands; D011108:Polymers; D012825:Silicon