title: The TCP4 transcription factor of Arabidopsis blocks cell division in yeast at G1→S transition.
abstract: The TCP transcription factors control important aspects of plant development. Members of class I TCP proteins promote cell cycle by regulating genes directly involved in cell proliferation. In contrast, members of class II TCP proteins repress cell division. While it has been postulated that class II proteins induce differentiation signal, their exact role on cell cycle has not been studied. Here, we report that TCP4, a class II TCP protein from Arabidopsis that repress cell proliferation in developing leaves, inhibits cell division by blocking G1→S transition in budding yeast. Cells expressing TCP4 protein with increased transcriptional activity fail to progress beyond G1 phase. By analyzing global transcriptional status of these cells, we show that expression of a number of cell cycle genes is altered. The possible mechanism of G1→S arrest is discussed.
authors.affiliation: Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560 012, India.
authors.forename: Pooja
authors.initials: P
authors.lastname: Aggarwal
authors.forename: Bhavna
authors.initials: B
authors.lastname: Padmanabhan
authors.forename: Abhay
authors.initials: A
authors.lastname: Bhat
authors.forename: Kavitha
authors.initials: K
authors.lastname: Sarvepalli
authors.forename: Parag P
authors.initials: PP
authors.lastname: Sadhale
authors.forename: Utpal
authors.initials: U
authors.lastname: Nath
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000502:physiology*; D002455:Cell Division / Q000235:genetics*; D016193:G1 Phase / Q000235:genetics; D016196:S Phase / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics / Q000254:growth & development*; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*
chemical_list: D029681:Arabidopsis Proteins; C560908:TCP4 protein, Arabidopsis; D014157:Transcription Factors