title: Deep small RNA sequencing from the nematode Ascaris reveals conservation, functional diversification, and novel developmental profiles.
abstract: Eukaryotic cells express several classes of small RNAs that regulate gene expression and ensure genome maintenance. Endogenous siRNAs (endo-siRNAs) and Piwi-interacting RNAs (piRNAs) mainly control gene and transposon expression in the germline, while microRNAs (miRNAs) generally function in post-transcriptional gene silencing in both somatic and germline cells. To provide an evolutionary and developmental perspective on small RNA pathways in nematodes, we identified and characterized known and novel small RNA classes through gametogenesis and embryo development in the parasitic nematode Ascaris suum and compared them with known small RNAs of Caenorhabditis elegans. piRNAs, Piwi-clade Argonautes, and other proteins associated with the piRNA pathway have been lost in Ascaris. miRNAs are synthesized immediately after fertilization in utero, before pronuclear fusion, and before the first cleavage of the zygote. This is the earliest expression of small RNAs ever described at a developmental stage long thought to be transcriptionally quiescent. A comparison of the two classes of Ascaris endo-siRNAs, 22G-RNAs and 26G-RNAs, to those in C. elegans, suggests great diversification and plasticity in the use of small RNA pathways during spermatogenesis in different nematodes. Our data reveal conserved characteristics of nematode small RNAs as well as features unique to Ascaris that illustrate significant flexibility in the use of small RNAs pathways, some of which are likely an adaptation to Ascaris' life cycle and parasitism. The transcriptome assembly has been submitted to NCBI Transcriptome Shotgun Assembly Sequence Database(http://www.ncbi.nlm.nih.gov/genbank/TSA.html) under accession numbers JI163767–JI182837 and JI210738–JI257410.
authors.affiliation: Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Aurora, CO 80045, USA.
authors.forename: Jianbin
authors.initials: J
authors.lastname: Wang
authors.forename: Benjamin
authors.initials: B
authors.lastname: Czech
authors.forename: Amanda
authors.initials: A
authors.lastname: Crunk
authors.forename: Adam
authors.initials: A
authors.lastname: Wallace
authors.forename: Makedonka
authors.initials: M
authors.lastname: Mitreva
authors.forename: Gregory J
authors.initials: GJ
authors.lastname: Hannon
authors.forename: Richard E
authors.initials: RE
authors.lastname: Davis
mesh_terms: D000818:Animals; D001200:Ascaris / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D016000:Cluster Analysis; D017124:Conserved Sequence; D047108:Embryonic Development / Q000235:genetics; D005718:Gametogenesis / Q000235:genetics; D015870:Gene Expression; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D014644:Genetic Variation; D059014:High-Throughput Nucleotide Sequencing; D055029:Inverted Repeat Sequences; D008969:Molecular Sequence Data; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism*; D017423:Sequence Analysis, RNA; D013091:Spermatogenesis / Q000235:genetics; D015053:Zygote / Q000378:metabolism
chemical_list: D034741:RNA, Small Interfering