title: Quantification of in vitro and in vivo angiogenesis stimulated by ovine forestomach matrix biomaterial.
abstract: Ovine forestomach matrix (OFM) biomaterial acts as a biomimetic of native extracellular matrix (ECM) by providing structural and functional cues to orchestrate cell activity during tissue regeneration. The ordered collagen matrix of the biomaterial is supplemented with secondary ECM-associated macromolecules that function in cell adhesion, migration and communication. As angiogenesis and vasculogenesis are critical processes during tissue regeneration we sought to quantify the angiogenic properties of the OFM biomaterial. In vitro studies demonstrated that soluble OFM components stimulated human umbilical vein endothelial cell (HUVEC) migration and increased vascular sprouting from an aorta. Blood vessel density and branch points increased in response to OFM in an ex ovo chicken chorioallantoic membrane (CAM) assay. The OFM biomaterial was shown to undergo remodeling in a porcine full-thickness excisional model and gave rise to significantly more blood vessels than wounds treated with small intestinal submucosa decellularized ECM or untreated wounds.
authors.affiliation: Mesynthes Limited, PO Box 31311, Lower Hutt 5040, New Zealand.
authors.forename: Sharleen M
authors.initials: SM
authors.lastname: Irvine
authors.forename: Juliet
authors.initials: J
authors.lastname: Cayzer
authors.forename: Elise M
authors.initials: EM
authors.lastname: Todd
authors.forename: Stan
authors.initials: S
authors.lastname: Lun
authors.forename: Evan W
authors.initials: EW
authors.lastname: Floden
authors.forename: Leonardo
authors.initials: L
authors.lastname: Negron
authors.forename: James N
authors.initials: JN
authors.lastname: Fisher
authors.forename: Sandi G
authors.initials: SG
authors.lastname: Dempsey
authors.forename: Alan
authors.initials: A
authors.lastname: Alexander
authors.forename: Michael C
authors.initials: MC
authors.lastname: Hill
authors.forename: Annalee
authors.initials: A
authors.lastname: O'Rouke
authors.forename: Sarah P
authors.initials: SP
authors.lastname: Gunningham
authors.forename: Cameron
authors.initials: C
authors.lastname: Knight
authors.forename: Paul F
authors.initials: PF
authors.lastname: Davis
authors.forename: Brian R
authors.initials: BR
authors.lastname: Ward
authors.forename: Barnaby C H
authors.initials: BC
authors.lastname: May
mesh_terms: D000818:Animals; D001011:Aorta / Q000187:drug effects / Q000502:physiology; D001672:Biocompatible Materials / Q000494:pharmacology*; D001681:Biological Assay; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002645:Chickens; D049033:Chorioallantoic Membrane / Q000098:blood supply / Q000187:drug effects; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects; D005109:Extracellular Matrix / Q000378:metabolism*; D005753:Gastric Mucosa / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D051381:Rats; D012038:Regeneration / Q000187:drug effects; D012756:Sheep; D014471:Umbilical Veins / Q000166:cytology
chemical_list: D001672:Biocompatible Materials