title: miR-29a and miR-142-3p downregulation and diagnostic implication in human acute myeloid leukemia.
abstract: Expression profiling of microRNAs (miRNAs) in most diseases might be popular and provide the possibility for diagnostic implication, but few studies have accurately quantified the expression level of dysregulated miRNAs in acute myeloid leukemia (AML). In this study, we analyzed the peripheral blood mononuclear cells (PBMCs) from 10 AML patients (subtypes M1 to M5) and six normal controls by miRNA microarray and identified several differentially expressed miRNAs. Among them miR-29a and miR-142-3p were selectively encountered in Northern blot analysis and their significantly decreased expression in AML was further confirmed. Quantitative real-time PCR in 52 primarily diagnosed AML patients and 100 normal controls not only verified the expression properties of these 2 miRNAs, but also established that the expression level of miR-142-3p and miR-29a in PBMCs could be used as novel diagnostic markers. A better diagnostic outcome was achieved by combining miR-29a and miR-142-3p with about 90% sensitivity, 100% specificity, and an area under the ROC curve (AUC) of 0.97. Our results provide insights into the involvement of miRNAs in leukemogenesis, and offer candidates for AML diagnosis and therapeutic strategy.
authors.affiliation: National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, People's Republic of China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Xiao-Shuang
authors.initials: XS
authors.lastname: Wang
authors.forename: Gui-Hua
authors.initials: GH
authors.lastname: Yang
authors.forename: Peng-Fei
authors.initials: PF
authors.lastname: Zhai
authors.forename: Zhen
authors.initials: Z
authors.lastname: Xiao
authors.forename: Liang-Yu
authors.initials: LY
authors.lastname: Xia
authors.forename: Li-Rong
authors.initials: LR
authors.lastname: Chen
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiao-Zhong
authors.initials: XZ
authors.lastname: Wang
authors.forename: Lai-Xi
authors.initials: LX
authors.lastname: Bi
authors.forename: Nian
authors.initials: N
authors.lastname: Liu
authors.forename: Yang
authors.initials: Y
authors.lastname: Yu
authors.forename: Da
authors.initials: D
authors.lastname: Gao
authors.forename: Bin-Tao
authors.initials: BT
authors.lastname: Huang
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Dao-Bin
authors.initials: DB
authors.lastname: Zhou
authors.forename: Jia-Nan
authors.initials: JN
authors.lastname: Gong
authors.forename: Hua-Lu
authors.initials: HL
authors.lastname: Zhao
authors.forename: Xiu-Hua
authors.initials: XH
authors.lastname: Bi
authors.forename: Jia
authors.initials: J
authors.lastname: Yu
authors.forename: Jun-Wu
authors.initials: JW
authors.lastname: Zhang
mesh_terms: D019540:Area Under Curve; D014408:Biomarkers, Tumor / Q000235:genetics*; D015152:Blotting, Northern; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D035683:MicroRNAs / Q000378:metabolism*; D046228:Microarray Analysis; D012372:ROC Curve; D060888:Real-Time Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D014408:Biomarkers, Tumor; C555159:MIRN142 microRNA, human; C532045:MIRN29a microRNA, human; D035683:MicroRNAs