title: Metabolic labeling of fucosylated glycoproteins in Bacteroidales species.
abstract: Members of the Bacteroidales order are among the most abundant gram-negative bacteria of the human colonic microbiota. These species decorate their cell-surface glycoproteins with fucosylated glycans, which are believed to play important roles in host intestinal colonization. Currently, there is no method for the enrichment of these glycoproteins for their identification. Here, we describe a chemical approach directed toward labeling and detecting fucosylated glycoproteins from cultured Bacteroidales species, namely Bacteroides fragilis and Parabacteroides distasonis. We treated these bacteria with an alkyne-bearing fucose analog, which is metabolically integrated into the bacterial surface fucosylated glycoproteins. The alkyne-tagged glycoproteins can then react with azide-bearing biophysical probes via bioorthogonal click chemistry for detection or glycoproteomic analysis.
authors.affiliation: Department of Biochemistry, Albert Einstein College of Medicine, Yeshiva University, 1300 Morris Park Ave., Bronx, NY 10461, USA.
authors.forename: Christen
authors.initials: C
authors.lastname: Besanceney-Webler
authors.forename: Hao
authors.initials: H
authors.lastname: Jiang
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Anthony D
authors.initials: AD
authors.lastname: Baughn
authors.forename: Peng
authors.initials: P
authors.lastname: Wu
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D001439:Bacteroides / Q000378:metabolism*; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D005643:Fucose / Q000737:chemistry*; D006023:Glycoproteins / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D006023:Glycoproteins; D005643:Fucose