title: Lipotoxicity in HepG2 cells triggered by free fatty acids.
abstract: The goal of this study was to investigate the lipid accumulation and lipotoxicity of free fatty acids (FFAs) induced in HepG2 cells. HepG2 cells were co-incubated with various concentrations of FFAs for 24h and the intracellular lipid contents were observed by Oil Red O and Nile Red staining methods. The lipotoxicity of HepG2 cells were then detected by Hoechest 33342/PI, Annexin V-FITC/PI double-staining and 3-(4,5-dimethylthiazol-2-yl)-2,5-di phenyltetrazolium bromide (MTT) experiment tests. The experiments showed a lipid accumulation and lipotoxicity by increasing FFA concentration gradients. Through cell morphological observation and quantitative analysis, FFAs have shown to increase in a dose-dependent manner compared with the control group. The data collected from hoechst 33342/PI, annexin V-FITC/PI double staining and also MTT experiments showed that cell apoptosis and necrosis significantly increased with increasing FFA concentrations. Apoptosis was not obvious in the 1 mM FFAs-treated group compared to the other two groups. In a certain concentration range, FFAs induced intracellular lipid accumulation and lipotoxicity of HepG2 cells in a dose-dependent manner.
authors.forename: Hong-Rui
authors.initials: HR
authors.lastname: Yao
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.forename: Daniel
authors.initials: D
authors.lastname: Plumeri
authors.forename: Yong-Bing
authors.initials: YB
authors.lastname: Cao
authors.forename: Ting
authors.initials: T
authors.lastname: He
authors.forename: Ling
authors.initials: L
authors.lastname: Lin
authors.forename: Yu
authors.initials: Y
authors.lastname: Li
authors.forename: Yuan-Ying
authors.initials: YY
authors.lastname: Jiang
authors.forename: Ji
authors.initials: J
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Shang