title: Characterization of a putative cis-regulatory element that controls transcriptional activity of the pig uroplakin II gene promoter.
abstract: Uroplakin II (UPII) is a one of the integral membrane proteins synthesized as a major differentiation product of mammalian urothelium. UPII gene expression is bladder specific and differentiation dependent, but little is known about its transcription response elements and molecular mechanism. To identify the cis-regulatory elements in the pig UPII (pUPII) gene promoter region, we constructed pUPII 5' upstream region deletion mutants and demonstrated that each of the deletion mutants participates in controlling the expression of the pUPII gene in human bladder carcinoma RT4 cells. We also identified a new core promoter region and putative negative cis-regulatory element within a minimal promoter region. In addition, we showed that hepatocyte nuclear factor 4 (HNF4) can directly bind in the pUPII core promoter (5F-1) region, which plays a critical role in controlling promoter activity. Transient cotransfection experiments showed that HNF4 positively regulates pUPII gene promoter activity. Thus, the binding element and its binding protein, HNF4 transcription factor, may be involved in the mechanism that specifically regulates pUPII gene transcription.
authors.affiliation: Department of Animal Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Deug-Nam
authors.initials: DN
authors.lastname: Kwon
authors.forename: Mi-Ryung
authors.initials: MR
authors.lastname: Park
authors.forename: Jong-Yi
authors.initials: JY
authors.lastname: Park
authors.forename: Ssang-Goo
authors.initials: SG
authors.lastname: Cho
authors.forename: Chankyu
authors.initials: C
authors.lastname: Park
authors.forename: Jae-Wook
authors.initials: JW
authors.lastname: Oh
authors.forename: Hyuk
authors.initials: H
authors.lastname: Song
authors.forename: Jae-Hwan
authors.initials: JH
authors.lastname: Kim
authors.forename: Jin-Hoi
authors.initials: JH
authors.lastname: Kim
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D024202:Electrophoretic Mobility Shift Assay; D051557:Hepatocyte Nuclear Factor 4 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D020218:Response Elements; D013552:Swine / Q000235:genetics*; D015533:Transcriptional Activation; D060170:Uroplakin II
chemical_list: D051557:Hepatocyte Nuclear Factor 4; D008565:Membrane Proteins; C554658:UPK2 protein, human; D060170:Uroplakin II