title: Field evaluation of five rapid diagnostic tests for screening of HIV-1 infections in rural Rakai, Uganda.
abstract: The performance characteristics of HIV rapid diagnostic tests (RDTs) vary by test and by population. We assessed five commercial RDTs in Uganda where all but one RDT (Determine; Abbott Laboratories, Germany) performed close to manufacturer's expectations. Determine had low specificity (85.2%, positive predictive value 67.3%) due to false-positive results with weak-positive bands. Properly trained staff, good quality control programmes and validation of RDTs with laboratories having confirmatory testing capacity may be warranted to assure accuracy in each setting.
authors.affiliation: Rakai Health Sciences Program, Kalisizo, Uganda.
authors.forename: S C
authors.initials: SC
authors.lastname: Kagulire
authors.forename: P
authors.initials: P
authors.lastname: Opendi
authors.forename: P D
authors.initials: PD
authors.lastname: Stamper
authors.forename: J L
authors.initials: JL
authors.lastname: Nakavuma
authors.forename: L A
authors.initials: LA
authors.lastname: Mills
authors.forename: F
authors.initials: F
authors.lastname: Makumbi
authors.forename: R H
authors.initials: RH
authors.lastname: Gray
authors.forename: J P
authors.initials: JP
authors.lastname: Shott
authors.forename: D
authors.initials: D
authors.lastname: Serwadda
authors.forename: S J
authors.initials: SJ
authors.lastname: Reynolds
mesh_terms: D015153:Blotting, Western; D015331:Cohort Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005189:False Positive Reactions; D015658:HIV Infections / Q000175:diagnosis* / Q000821:virology; D015497:HIV-1 / Q000302:isolation & purification*; D006801:Humans; D019095:Point-of-Care Systems; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D012424:Rural Population; D012680:Sensitivity and Specificity; D014454:Uganda
chemical_list: D011933:Reagent Kits, Diagnostic