title: The cellular prion protein is expressed in olfactory sensory neurons of adult mice but does not affect the early events of the olfactory transduction pathway.
abstract: A conformational conversion of the cellular prion protein (PrP(C)) is now recognized as the causal event of fatal neurodegenerative disorders, known as prion diseases. In spite of long-lasting efforts, however, the physiological role of PrP(C) remains unclear. It has been reported that PrP(C) is expressed in various areas of the olfactory system, including the olfactory epithelium, but its precise localization in olfactory sensory neurons (OSNs) is still debated. Here, using immunohistochemistry tools, we have reinvestigated the expression and localization of PrP(C) in the olfactory epithelium of adult congenic mice expressing different PrP(C) amounts, that is, wild-type, PrP-knockout, and transgenic PrP(C)-overexpressing animals. We found that PrP(C) was expressed in OSNs, in which, however, it was unevenly distributed, being detectable at low levels in cell bodies, dendrites and apical layer, and more abundantly in axons. We also studied the involvement of PrP(C) in the response of the olfactory epithelium to odorants, by comparing the electro-olfactograms of the 3 mouse lines subjected to different stimulation protocols. We found no significant difference between the 3 PrP genotypes, supporting previous reports that exclude a direct action of PrP(C) in the early signal transduction activity of the olfactory epithelium.
authors.affiliation: SISSA, International School for Advanced Studies and Italian Institute of Technology, SISSA Unit, Trieste, Italy.
authors.forename: Michele
authors.initials: M
authors.lastname: Dibattista
authors.forename: Maria Lina
authors.initials: ML
authors.lastname: Massimino
authors.forename: Devendra Kumar
authors.initials: DK
authors.lastname: Maurya
authors.forename: Anna
authors.initials: A
authors.lastname: Menini
authors.forename: Alessandro
authors.initials: A
authors.lastname: Bertoli
authors.forename: M Catia
authors.initials: MC
authors.lastname: Sorgato
mesh_terms: D000818:Animals; D055786:Gene Knockout Techniques; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D009831:Olfactory Mucosa / Q000378:metabolism; D018621:PrPC Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D011984:Sensory Receptor Cells / Q000378:metabolism*; D015398:Signal Transduction; D012903:Smell / Q000502:physiology*
chemical_list: D018621:PrPC Proteins