title: A novel technique for the transplantation of pancreatic islets within a vascularized device into the greater omentum to achieve insulin independence.
abstract: BACKGROUND
  The greater omentum with its vascularization and blood flow has been considered as a location for islet transplantation; however, there is a need to provide a controlled and protected site for the islets within the omentum that would be applicable to donor islets and future stem cell technologies. Here we describe the use of a novel device implanted within the omentum with a subcutaneous delivery port that offers an environment for donor islets.
  
  
  METHODS
  A prototype cell pouch device was wrapped in the greater omentum and an islet implantation port was exposed subcutaneously in diabetic Lewis rats. After tissue growth throughout the device, islet isografts were implanted and long-term glucose control was evaluated.
  
  
  RESULTS
  By using this technique, 7 of 10 diabetic rat recipients showed long-term normal blood glucose levels after minimal islet dose transplants. Histologic assessment revealed collagen formation and vascularization within the device.
  
  
  CONCLUSIONS
  The implanted device assessed using this technique provides a safe and efficacious environment for the support of pancreatic islets contained within a removable device as a cell therapy in a highly vascularized setting.
authors.affiliation: Robarts Research Institute, University of Western Ontario, London, Ontario, Canada.
authors.forename: Jan
authors.initials: J
authors.lastname: Kriz
authors.forename: Greg
authors.initials: G
authors.lastname: Vilk
authors.forename: Delfina M
authors.initials: DM
authors.lastname: Mazzuca
authors.forename: Philip M
authors.initials: PM
authors.lastname: Toleikis
authors.forename: Paula J
authors.initials: PJ
authors.lastname: Foster
authors.forename: David J G
authors.initials: DJ
authors.lastname: White
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D001786:Blood Glucose / Q000378:metabolism; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000601:surgery*; D005260:Female; D016381:Islets of Langerhans Transplantation / Q000295:instrumentation / Q000379:methods*; D008297:Male; D009852:Omentum / Q000601:surgery*; D051381:Rats; D016896:Treatment Outcome
chemical_list: D015415:Biomarkers; D001786:Blood Glucose