title: Comparative analyses of chromatographic fingerprints of the roots of Polygonum multiflorum Thunb. and their processed products using RRLC/DAD/ESI-MS(n).
abstract: The dried roots of Polygonum multiflorum Thunb. (Heshouwu) and their processed products (Zhi-heshouwu) are widely used in traditional Chinese medicine, yet their therapeutic effects are different. Previous investigations focused mainly on the differences between Heshouwu and Zhi-heshouwu in the contents of several known compounds. In this study, a rapid resolution liquid chromatography-diode array detection/electrospray ionization tandem mass spectrometry (RRLC/DAD/ESI-MS(n)) method was developed for the comparative analysis of the components of Heshouwu and Zhi-heshouwu. A total of 23 compounds were identified or tentatively characterized. We found that 16 batches of Heshouwu and 15 batches of Zhi-heshouwu samples shared eight compounds, including gallic acid; 3,5,4'-tetrahydroxylstilbene-2,3-di-O-glucoside, CIS-2,3,5,4'-tetrahydroxylstilbene-2-O- β-D-glucoside, trans-2,3,5,4'-tetrahydroxylstilbene-2-O- β-D-glucoside, emodin-8-O- β-D-glucoside, physcion-8-O- β-D-glucoside, emodin, and physcion. Nevertheless, the relative amounts of gallic acid, emodin, and physcion were very high in Zhi-heshouwu samples compared to those in Heshouwu samples. Six compounds disappeared after processing and were unique for Heshouwu: catechin, flavanol gallate dimer, polygonimitin B, emodin-1-O-glucoside, emodin-8-O-(6'-O-malonyl)-glucoside, and physcion-8-O-(6'-O-malonyl)-glucoside. Three compounds were unique for Zhi-heshouwu: hydroxymaltol, 2,3-dihydro-3,5-dihydroxy-6-methyl-4(H)-pyran-4-one, and 5-hydroxymethyl furfural. These results suggest that the types and relative amounts of the chemical components of Heshouwu and Zhi-heshouwu are different.
authors.affiliation: Institution of Basic Theory, China Academy of Chinese Medical Sciences, Beijing, China.
authors.forename: Zhenli
authors.initials: Z
authors.lastname: Liu
authors.forename: Yuanyan
authors.initials: Y
authors.lastname: Liu
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.forename: Na
authors.initials: N
authors.lastname: Guo
authors.forename: Zhiqian
authors.initials: Z
authors.lastname: Song
authors.forename: Chun
authors.initials: C
authors.lastname: Wang
authors.forename: Lei
authors.initials: L
authors.lastname: Xia
authors.forename: Aiping
authors.initials: A
authors.lastname: Lu
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification; D004642:Emodin / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification; D005662:Furaldehyde / Q000737:chemistry / Q000302:isolation & purification; D005707:Gallic Acid / Q000737:chemistry / Q000302:isolation & purification; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification; D008516:Medicine, Chinese Traditional; D018517:Plant Roots / Q000737:chemistry; D028502:Polygonum / Q000737:chemistry*; D011753:Pyrones / Q000737:chemistry / Q000302:isolation & purification; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry
chemical_list: C579597:2,3-dihydro-3,5-dihydroxy-6-methyl-4(H)-pyran-4-one; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D005960:Glucosides; D011753:Pyrones; D005707:Gallic Acid; D005662:Furaldehyde; C008905:physcione; D004642:Emodin