title: Gene-class analysis of expression patterns induced by psychoactive pharmaceutical exposure in fathead minnow (Pimephales promelas) indicates induction of neuronal systems.
abstract: Psychoactive pharmaceuticals are among the most frequently prescribed drugs, contributing to persistent measurable concentrations in aquatic systems. Typically, it is assumed that such contaminants have no human health implications because they exist in extremely low concentrations. We exposed juvenile fathead minnows (Pimephales promelas) to three pharmaceuticals, fluoxetine, venlafaxine and carbamazepine, individually and in a mixture, and measured their effect on the induction of gene expression in fish brains using microarray analysis. Gene expression changes were accompanied by behavioral changes and validated by qPCR analysis. Gene Set Enrichment Analysis was used to perform gene-class analysis of gene expression, testing for enrichment of gene sets known to be involved in human neuronal development, regulation and growth. We found significant enrichment of gene sets for each of the treatments, with the largest induction of expression by the mixture treatment. These results suggest that the psychoactive pharmaceuticals are able to alter expression of fish genes associated with development, regulation and differentiation of synapses, neurons and neurotransmitters. The results provide a new perspective for the consideration of potential consequence for human health due to environmental exposure to unmetabolized psychoactive pharmaceuticals.
authors.affiliation: Department of Biological Sciences, Idaho State University, Stop 8007, 921 S 8th Ave, Pocatello, ID 83209-8007, USA. mthomas@isu.edu
authors.forename: Michael A
authors.initials: MA
authors.lastname: Thomas
authors.forename: Parag P
authors.initials: PP
authors.lastname: Joshi
authors.forename: Rebecca D
authors.initials: RD
authors.lastname: Klaper
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D002220:Carbamazepine / Q000494:pharmacology; D003511:Cyclohexanols / Q000494:pharmacology; D003530:Cyprinidae / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D005260:Female; D005473:Fluoxetine / Q000494:pharmacology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D011619:Psychotropic Drugs / Q000009:adverse effects* / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000069470:Venlafaxine Hydrochloride
chemical_list: D003511:Cyclohexanols; D011619:Psychotropic Drugs; D005473:Fluoxetine; D002220:Carbamazepine; D000069470:Venlafaxine Hydrochloride