title: The extraction process optimization and physicochemical properties of polysaccharides from the roots of Euphorbia fischeriana.
abstract: Response surface methodology (RSM) was used to determine the optimum extraction conditions for polysaccharides (EFP) from the roots of Euphorbia fischeriana. A Box-Behnken design (BBD) with four independent variables was investigated, such as extraction temperature (°C), water/solid ratio, extraction number (n), and extraction time (h). The results indicated optimum extraction conditions were extraction temperature of 97°C, water/solid ratio of 9:1, extraction number of 2 and extraction time of 2.4h, respectively. Under these conditions, the experimental value was 24.6±0.62, which was well in close agreement with value predicted by the model. The preliminary chemical analysis of EFP revealed the EFP contained 25.43% polysaccharides, 20.42% uronic acids, 2.54% sulfate radical and 23.41% proteins. And the neutral polysaccharides were mainly composed of glucose, arabinose, rhamnose, galactose, xylose, mannose in the ratio of 21:8:5:3:1:1.
authors.affiliation: Qiqihar Medical University, Qiqihar 161006, China. jichengliu@yahoo.cn
authors.forename: Jicheng
authors.initials: J
authors.lastname: Liu
authors.forename: Yongxu
authors.initials: Y
authors.lastname: Sun
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.forename: Chunlei
authors.initials: C
authors.lastname: Yu
mesh_terms: D005591:Chemical Fractionation / Q000379:methods*; D055598:Chemical Phenomena; D028481:Euphorbia / Q000737:chemistry*; D015233:Models, Statistical; D018517:Plant Roots / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification*; D015203:Reproducibility of Results; D013696:Temperature; D013997:Time Factors; D014867:Water / Q000737:chemistry
chemical_list: D011134:Polysaccharides; D014867:Water