title: The changes of T lymphocytes and cytokines in ICR mice fed with Fe3O4 magnetic nanoparticles.
abstract: The aim of this article is to study the changes inhibited T lymphocytes and cytokines related to the cellular immunity in ICR (imprinting control region) mice fed with Fe(3)O(4) magnetic nanoparticles (Fe(3)O(4)-MNPs). The Fe(3)O(4)-MNPs were synthesized, and their characteristics such as particle size, zeta potential, and X-ray diffraction patterns were measured and determined. All ICR mice were sacrificed after being exposed to 0, 300, 600, and 1200 mg/kg of Fe(3)O(4)-MNPs by single gastric administration for 14 days. Splenocytes proliferation was indicated with stimulate index by MTT assay; release of cytokines in the serum of ICR mice was detected by enzyme-linked immunosorbent assay, and the phenotypic analyses of T-lymphocyte subsets were performed using flow cytometry. Our results indicated that there were no significant differences in splenocyte proliferation and release of cytokines between exposed and control groups. Furthermore, there was no significant difference in the proportions of T-lymphocyte subsets in the low-dose Fe(3)O(4)-MNPs group when compared to the control group, but the proportions of CD3(+)CD4(+) and CD3(+)CD8(+) T-lymphocyte subsets both in the medium- and high-dose Fe(3)O(4)-MNPs groups were higher than those in the control group. It is concluded that a high dose of Fe(3)O(4)-MNPs, to some extent, could influence in vivo immune function of normal ICR mice.
authors.affiliation: Department of Hematology, Zhongda Hospital, Southeast University, Nanjing, People’s Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Baoan
authors.initials: B
authors.lastname: Chen
authors.forename: Nan
authors.initials: N
authors.lastname: Jin
authors.forename: Guohua
authors.initials: G
authors.lastname: Xia
authors.forename: Yue
authors.initials: Y
authors.lastname: Chen
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhou
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Cai
authors.forename: Jiahua
authors.initials: J
authors.lastname: Ding
authors.forename: Xiaomao
authors.initials: X
authors.lastname: Li
authors.forename: Xuemei
authors.initials: X
authors.lastname: Wang
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D048708:Cell Growth Processes / Q000187:drug effects; D016207:Cytokines / Q000097:blood*; D005260:Female; D058185:Magnetite Nanoparticles / Q000008:administration & dosage*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010316:Particle Size; D013154:Spleen / Q000166:cytology; D018709:Statistics, Nonparametric; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000276:immunology*; D014961:X-Ray Diffraction
chemical_list: D016207:Cytokines; D058185:Magnetite Nanoparticles