title: C-myc expression in the microvessels of medulloblastoma.
abstract: The increased expression of c-myc is related to neoplastic transformation and angiogenesis. Therefore, the assessment of expression of c-myc in endothelial cells and neovascularization could help to determine the biological behavior of the tumor. We analyzed neovascularization and c-myc expression in 36 medulloblastoma specimens. The results were shown by determining immunohistochemical staining index (ISI), the sum of staining intensity (SI) and the percentage of positive cells (PPC) in the blood vessels endothelium of the tumor. We also performed the microvessel count (MVC) in 10 high-power fields (400X) with the most prominent vascularization and expressed it as microvessel density per mm2 (MVD). C-myc immunostaining intensity index in blood vessel endothelium is grouped into four groups, 0--no reaction, I-weak reaction (ISI = 1 or 2), II--moderate reaction (ISI = 3 or 4), III--strong reaction (ISI = 5 or 6). Statistically significant differences (p = 0.0214) have been found between groups 0 and 1 compared to groups 2 and 3. A higher percentage of positive cells has been found in male patients than in female ones (p = 0.0483). C-myc PPC 0 or 1 has on the average smaller density of blood vessels per mm2 than c-myc PPC 2 or 3, but the difference is not statistically significant. C-myc ISI 0 or 1 has, on the average, smaller density of blood vessels per mm2 than c-myc ISI 2 or 3, but the difference is not statistically significant. We concluded that c-myc staining intensity was associated with higher microvessels density.
authors.affiliation: University of Zagreb, Sveti Duh University Hospital, Department of Pathology and Cytology, Zagreb, Croatia.
authors.forename: Gabrijela
authors.initials: G
authors.lastname: Stanić
authors.forename: Hrvoje
authors.initials: H
authors.lastname: Cupić
authors.forename: Kamelija
authors.initials: K
authors.lastname: Zarković
authors.forename: Davor
authors.initials: D
authors.lastname: Tomas
authors.forename: Bozo
authors.initials: B
authors.lastname: Kruslin
mesh_terms: D004730:Endothelium, Vascular / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D008527:Medulloblastoma / Q000098:blood supply* / Q000378:metabolism; D055806:Microvessels / Q000378:metabolism; D009389:Neovascularization, Pathologic / Q000378:metabolism; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis*
chemical_list: C489427:MYC protein, human; D016271:Proto-Oncogene Proteins c-myc