title: Quantitative analysis of optic disc color.
abstract: OBJECTIVE
  To evaluate the reproducibility of ImageJ software in analyzing the color of the optic disc.
  
  
  METHODS
  One hundred twelve normal participants (56 males and 56 females) were enrolled in this study. The image of the optic disc was taken using Kowa digital disc photo-graphy, and the gray scales of the nasal rim (NR), brightest cupping center (BCC) and largest inferior retinal vein (IRV) were calculated using histogram in ImageJ. Three different observers calculated the gray scales three separate times. Reproducibility was assessed using the interclass correlation coefficient (ICC).
  
  
  RESULTS
  The mean age of the participants was 50.6 years old (range, 11 to 82 years). The mean gray scales of the nasal rim were 91.81, 94.91, and 93.24; those of the brightest cupping center were 174.84, 179.94, and 177.76; and those of the largest inferior retinal vein were 61.85, 53.48, and 56.73 for observers 1, 2, and 3, respectively. Inter-observer reproducibility for NR, BCC and IRV was considered good based upon ICC values of 0.944, 0.860, and 0.789 for observers 1, 2, and 3, respectively. Significant age-related differences between the values of the brightest cupping center were noted, and the gray scale score was decreased in the older participants (p < 0.001).
  
  
  CONCLUSIONS
  The gray scale of the brightest cupping center diminished with age. ImageJ can be a useful objective tool with high reproducibility in the analysis of optic disc color.
authors.affiliation: Department of Ophthalmology, Kim's Eye Hospital, Konyang University College of Medicine, Seoul, Korea.
authors.forename: Ungsoo Samuel
authors.initials: US
authors.lastname: Kim
authors.forename: Sun Jung
authors.initials: SJ
authors.lastname: Kim
authors.forename: Seung-Hee
authors.initials: SH
authors.lastname: Baek
authors.forename: Hwang-Ki
authors.initials: HK
authors.lastname: Kim
authors.forename: Yong-Ho
authors.initials: YH
authors.lastname: Sohn
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D003116:Color; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008297:Male; D008875:Middle Aged; D009898:Optic Disk / Q000502:physiology*; D009929:Organ Size; D010781:Photography; D012016:Reference Values; D015203:Reproducibility of Results; D012169:Retinal Vein / Q000033:anatomy & histology; D012984:Software; D055815:Young Adult