title: Organization of ribonucleoside diphosphate reductase during multifork chromosome replication in Escherichia coli.
abstract: Ribonucleoside diphosphate reductase (RNR) is located in discrete foci in a number that increases with the overlapping of replication cycles in Escherichia coli. Comparison of the numbers of RNR, DnaX and SeqA protein foci with the number of replication forks at different growth rates reveals that fork : focus ratios augment with increasing growth rates, suggesting a higher cohesion of the three protein foci with increasing number of forks per cell. Quantification of NrdB and SeqA proteins per cell showed: (i) a higher amount of RNR per focus at faster growth rates, which sustains the higher cohesion of RNR foci with higher numbers of forks per cell; and (ii) an equivalent amount of RNR per replication fork, independent of the number of the latter.
authors.affiliation: School of Biosciences, The University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.|Departamento de Bioquímica y Biología Molecular y Genética, Universidad de Extremadura, Badajoz E06080, Spain.
authors.forename: María Antonia
authors.initials: MA
authors.lastname: Sánchez-Romero
authors.affiliation: Departamento de Bioquímica y Biología Molecular y Genética, Universidad de Extremadura, Badajoz E06080, Spain.
authors.forename: Felipe
authors.initials: F
authors.lastname: Molina
authors.affiliation: Departamento de Bioquímica y Biología Molecular y Genética, Universidad de Extremadura, Badajoz E06080, Spain.
authors.forename: Alfonso
authors.initials: A
authors.lastname: Jiménez-Sánchez
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000032:analysis; D001426:Bacterial Proteins / Q000032:analysis; D002455:Cell Division; D002875:Chromosomes / Q000378:metabolism*; D003593:Cytoplasm / Q000737:chemistry; D004258:DNA Polymerase III / Q000032:analysis; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000032:analysis; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000032:analysis; D012262:Ribonucleoside Diphosphate Reductase / Q000378:metabolism*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C103076:DnaX protein, Bacteria; D029968:Escherichia coli Proteins; C089746:SeqA protein, E coli; D012262:Ribonucleoside Diphosphate Reductase; D004258:DNA Polymerase III