title: Association between mannose-binding lectin and interleukin-1 receptor antagonist gene polymorphisms and recurrent vulvovaginal candidiasis.
abstract: OBJECTIVE
  The influence of functional polymorphisms in the genes coding for mannose-binding lectin (MBL) and interleukin-1 receptor antagonist (IL-1ra) on recurrent vulvovaginal candidiasis (RVVC) were examined in an urban Brazilian population.
  
  
  METHODS
  DNA was isolated from buccal swabs of 100 women with RVVC and 100 control women and tested by gene amplification for a single nucleotide polymorphism in codon 54 of the MBL2 gene and for a length polymorphism in intron 2 of the IL1RN gene. Genotype and allele frequencies were compared between groups.
  
  
  RESULTS
  The frequency of the variant MBL2 B allele, associated with reduced circulating and vaginal MBL concentrations, was 27.0% in RVVC and 8.5% in control women (p < .0001). The MBL2 B,B genotype was present in 12% of RVVC patients and 1% of controls (p = .0025). The IL1RN 2 allele frequency, associated with the highest level of unopposed IL-1β activity, was 24.0% in RVVC and 23.4% in controls. The IL1RN genotype distribution was also similar in both groups.
  
  
  CONCLUSIONS
  Carriage of the MBL2 codon 54 polymorphism, but not the IL1RN length polymorphism, predisposes to RVVC in Brazilian women.
authors.affiliation: Serviço de Imunologia, Genetica e Infecções do Trato Reprodutivo da Disciplina de Ginecologia, da Faculdade de Medicina, da Universidade de São Paulo, Avenida Dr. Eneas de Carvalho Aguiar 255, 10 andar, sala 10177, Cerkueira Cesar, Sao Paulo, SP, 05403900, Brazil.
authors.forename: Maria Dulce Kaoro Horie
authors.initials: MD
authors.lastname: Wojitani
authors.forename: Lana Maria
authors.initials: LM
authors.lastname: de Aguiar
authors.forename: Edmond Chada
authors.initials: EC
authors.lastname: Baracat
authors.forename: Iara Moreno
authors.initials: IM
authors.lastname: Linhares
mesh_terms: D000293:Adolescent; D000328:Adult; D001938:Brazil; D002181:Candidiasis, Vulvovaginal / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein / Q000235:genetics*; D007438:Introns; D037601:Mannose-Binding Lectin / Q000235:genetics*; D008875:Middle Aged; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D055815:Young Adult
chemical_list: C507763:IL1RN protein, human; D053590:Interleukin 1 Receptor Antagonist Protein; C445619:MBL2 protein, human; D037601:Mannose-Binding Lectin