title: A novel recombinant pseudorabies virus expressing parvovirus VP2 gene: Immunogenicity and protective efficacy in swine.
abstract: BACKGROUND
  Porcine parvovirus (PPV) VP2 gene has been successfully expressed in many expression systems resulting in self-assembly of virus-like particles (VLPs) with similar morphology to the native capsid. Here, a pseudorabies virus (PRV) system was adopted to express the PPV VP2 gene.
  
  
  METHODS
  A recombinant PRV SA215/VP2 was obtained by homologous recombination between the vector PRV viral DNA and a transfer plasmid. Then recombinant virus was purified with plaque purification, and its identity confirmed by PCR amplification, Western blot and indirect immunofluorescence (IFA) analyses. Electronic microscopy of PRV SA215/VP2 confirmed self-assembly of both pseudorabies virus and VLPs from VP2 protein.
  
  
  RESULTS
  Immunization of piglets with recombinant virus elicited PRV-specific and PPV-specific humoral immune responses and provided complete protection against a lethal dose of PRV challenges. Gilts immunized with recombinant viruses induced PPV-specific antibodies, and significantly reduced the mortality rate of (1 of 28) following virulent PPV challenge compared with the control (7 of 31). Furthermore, PPV virus DNA was not detected in the fetuses of recombinant virus immunized gilts.
  
  
  CONCLUSIONS
  In this study, a recombinant PRV SA215/VP2 virus expressing PPV VP2 protein was constructed using PRV SA215 vector. The safety, immunogenicity, and protective efficacy of the recombinant virus were demonstrated in piglets and primiparous gilts. This recombinant PRV SA215/VP2 represents a suitable candidate for the development of a bivalent vaccine against both PRV and PPV infection.
authors.affiliation: Animal Biotechnology Center of Sichuan Agricultural University, Ya'an, Sichuan, 625014, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Chen
authors.forename: Wanzhu
authors.initials: W
authors.lastname: Guo
authors.forename: Zhiwen
authors.initials: Z
authors.lastname: Xu
authors.forename: Qigui
authors.initials: Q
authors.lastname: Yan
authors.forename: Yan
authors.initials: Y
authors.lastname: Luo
authors.forename: Qian
authors.initials: Q
authors.lastname: Shi
authors.forename: Dishi
authors.initials: D
authors.lastname: Chen
authors.forename: Ling
authors.initials: L
authors.lastname: Zhu
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology*; D036022:Capsid Proteins / Q000235:genetics / Q000276:immunology*; D004337:Drug Carriers; D015870:Gene Expression; D005822:Genetic Vectors; D011558:Herpesvirus 1, Suid / Q000235:genetics*; D010322:Parvoviridae Infections / Q000517:prevention & control / Q000662:veterinary*; D028323:Parvovirus, Porcine / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D016019:Survival Analysis; D013552:Swine; D013553:Swine Diseases / Q000517:prevention & control* / Q000821:virology; D014611:Vaccination / Q000379:methods; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000009:adverse effects / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000009:adverse effects / Q000235:genetics / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; D004337:Drug Carriers; C528774:VP2 protein, porcine parvovirus; D014614:Vaccines, Synthetic; D014765:Viral Vaccines