title: Genome-wide mapping of ZNF652 promoter binding sites in breast cancer cells.
abstract: A significant proportion of transcription factors encoded by the human genome are classical C(2) H(2) zinc finger proteins that regulate gene expression by directly interacting with their cognate DNA binding motifs. We previously showed that one such C(2) H(2) zinc finger DNA binding protein, ZNF652 (zinc finger protein 652), specifically and functionally interacts with CBFA2T3 to repress transcription of genes involved in breast oncogenesis. To identify potential targets by which ZNF652 exerts its putative tumour suppressive function, its promoter-specific cistrome was mapped by ChIP-chip. De novo motif scanning of the ZNF652 binding sites identified a novel ZNF652 recognition motif that closely resembles the previously characterised in vitro binding site, being a 10 nucleotide core of that 13 nucleotide sequence. Genes with ZNF652 binding sites function in diverse cellular pathways, and many are involved in cancer development and progression. Characterisation of the in vivo ZNF652 DNA binding motif and identification of potential ZNF652 target genes are key steps towards elucidating the function(s) of this transcription factor in the normal and malignant breast cell.
authors.affiliation: Breast Cancer Genetics Group, Discipline of Medicine, University of Adelaide and Hanson Institute, SA Pathology, Adelaide, SA, Australia. raman.sharma@adelaide.edu.au
authors.forename: Raman
authors.initials: R
authors.lastname: Kumar
authors.forename: Luke A
authors.initials: LA
authors.lastname: Selth
authors.forename: Renee B
authors.initials: RB
authors.lastname: Schulz
authors.forename: Bee Suan
authors.initials: BS
authors.lastname: Tay
authors.forename: Paul M
authors.initials: PM
authors.lastname: Neilsen
authors.forename: David F
authors.initials: DF
authors.lastname: Callen
mesh_terms: D001665:Binding Sites / Q000235:genetics; D001943:Breast Neoplasms / Q000235:genetics*; D045744:Cell Line, Tumor; D002843:Chromatin / Q000378:metabolism; D047369:Chromatin Immunoprecipitation; D002874:Chromosome Mapping / Q000379:methods*; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D006836:Hydro-Lyases / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011485:Protein Binding; D012097:Repressor Proteins / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism; D025521:Tumor Suppressor Proteins / Q000378:metabolism
chemical_list: C115744:CBFA2T3 protein, human; D002843:Chromatin; D004268:DNA-Binding Proteins; C473143:GRHL3 protein, human; D012097:Repressor Proteins; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins; C525993:ZNF652 protein, human; D006836:Hydro-Lyases; C022479:pseudouridylate synthetase