title: Evaluation of tissue reaction, cell viability and cytokine production induced by Sealapex Plus.
abstract: OBJECTIVE
  The aim of this study was to investigate the effects of mineral trioxide aggregate (MTA), Sealapex, and a combination of Sealapex and MTA (Sealapex Plus) on the reaction of subcutaneous connective tissue of rats, and on cell viability and cytokine production in mouse fibroblasts.
  
  
  METHODS
  The tissue reaction was carried out with dentin tubes containing the materials implanted in the dorsal connective tissue of rats. The histological analysis was performed after 7 and 30 days. Millipore culture plate inserts with polyethylene tubes filled with materials were placed into 24-well cell culture plates with mouse fibroblasts to evaluate the cell viability by MTT assay. ELISA assays were also performed after 24 h of exposure of the mouse fibroblasts to set material disks.
  
  
  RESULTS
  Histopathologic examination showed Von Kossa-positive granules that were birefringent to polarized light for all the studied materials at the tube openings. No material inhibited the cell viability in the in vitro test. It was detected IL-6 production in all root-end filling materials. MTA and Sealapex Plus induced a slight raise of mean levels of IL-1β.
  
  
  CONCLUSIONS
  The results suggest that Sealapex Plus is biocompatible and stimulates the mineralization of the tissue.
authors.affiliation: Department of Endodontics, Araçatuba Dental School, São Paulo State University, Araçatuba, SP, Brazil. joao@foa.unesp.br
authors.forename: João Eduardo
authors.initials: JE
authors.lastname: Gomes-Filho
authors.forename: Alessandra Cristina
authors.initials: AC
authors.lastname: Gomes
authors.forename: Simone
authors.initials: S
authors.lastname: Watanabe
authors.forename: Sandra Helena Penha de
authors.initials: SH
authors.lastname: Oliveira
authors.forename: Pedro Felício Estrada
authors.initials: PF
authors.lastname: Bernabé
authors.forename: Célio
authors.initials: C
authors.lastname: Percinoto
mesh_terms: D017607:Aluminum Compounds / Q000494:pharmacology*; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology; D017610:Calcium Compounds / Q000494:pharmacology*; D002126:Calcium Hydroxide / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003238:Connective Tissue / Q000187:drug effects*; D016207:Cytokines / Q000187:drug effects*; D003804:Dentin / Q000187:drug effects; D004338:Drug Combinations; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts / Q000187:drug effects*; D008297:Male; D008422:Materials Testing; D010087:Oxides / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012387:Root Canal Filling Materials / Q000494:pharmacology*; D012459:Salicylates / Q000494:pharmacology*; D017640:Silicates / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D017607:Aluminum Compounds; D001672:Biocompatible Materials; D017610:Calcium Compounds; D016207:Cytokines; D004338:Drug Combinations; D010087:Oxides; D012387:Root Canal Filling Materials; D012459:Salicylates; D017640:Silicates; C086631:mineral trioxide aggregate; C055601:Sealapex; D002126:Calcium Hydroxide