title: Occurrence of two distinct Theileria lineages in sika deer (Cervus nippon) of Iwate Prefecture, Japan.
abstract: In the present study, we tried to detect protozoan blood parasites from the liver or blood of 141 Japanese sika deer (Cervus nippon) in Iwate Prefecture of Japan by polymerase chain reaction. Approximately 500-bp amplicons were obtained in 76 (53.9%) of 141 samples by amplification for V4 hyper-variable regions of the 18S rRNA gene, and the amplicons were considered to be DNA of Theileria species. The complete nucleotide sequences (1701-bp) of the 18S rRNA gene were determined in 25 samples and were divided into 8 genotypes that were phylogenetically separated into two distinct lineages showing a monophyletic relation. The two lineages of Theileria were detected in different rates (12 and 88%) from sika deer in Iwate Prefecture.
authors.affiliation: Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, Morioka, Japan.
authors.forename: Kazuhito
authors.initials: K
authors.lastname: Ikawa
authors.forename: Mikiko
authors.initials: M
authors.lastname: Aoki
authors.forename: Madoka
authors.initials: M
authors.lastname: Ichikawa
authors.forename: Tadashi
authors.initials: T
authors.lastname: Itagaki
mesh_terms: D000818:Animals; D001483:Base Sequence; D016054:DNA, Protozoan / Q000737:chemistry*; D003670:Deer / Q000469:parasitology*; D007564:Japan; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000662:veterinary; D016794:Theileria / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D016054:DNA, Protozoan; D012337:RNA, Ribosomal, 18S