title: Comparative analysis of microbial DNA extraction protocols for groundwater samples.
abstract: A comparative analysis of four different DNA extraction protocols was performed to determine the best choice for groundwater microbial diversity studies using temperature gradient gel electrophoresis (TGGE) analysis. The methods used were a chelex-based method, a modified salting out procedure (MSOP), and the commercial kits Epicentre and FastDNA. Both commercial kits exhibited the greatest reproducibility in their methods; however, their band patterns were very different. The protocol that showed the highest diversity was the chelex-based method, and the one that showed the lowest diversity was the FastDNA kit.
authors.affiliation: Environmental Microbiology Group, Institute of Water Research, University of Granada, 18071 Granada, Spain.
authors.forename: Jessica
authors.initials: J
authors.lastname: Purswani
authors.forename: Antonio Manuel
authors.initials: AM
authors.lastname: Martín-Platero
authors.forename: Patricia
authors.initials: P
authors.lastname: Reboleiro-Rivas
authors.forename: Jesús
authors.initials: J
authors.lastname: González-López
authors.forename: Clementina
authors.initials: C
authors.lastname: Pozo
mesh_terms: D004247:DNA / Q000032:analysis / Q000302:isolation & purification*; D058645:Denaturing Gradient Gel Electrophoresis / Q000379:methods*; D005374:Filtration / Q000379:methods; D005618:Fresh Water / Q000382:microbiology*; D011137:Polystyrenes / Q000737:chemistry; D011145:Polyvinyls / Q000737:chemistry; D012492:Salts / Q000737:chemistry; D013010:Sonication
chemical_list: D011137:Polystyrenes; D011145:Polyvinyls; D012492:Salts; C006960:chelex; D004247:DNA