title: Acute reduction of serum 8-iso-PGF2-alpha and advanced oxidation protein products in vivo by a polyphenol-rich beverage; a pilot clinical study with phytochemical and in vitro antioxidant characterization.
abstract: BACKGROUND
  Measuring the effects of the acute intake of natural products on human biomarker concentrations, such as those related to oxidation and inflammation, can be an advantageous strategy for early clinical research on an ingredient or product.
  
  
  METHODS
  31 total healthy subjects were randomized in a double-blinded, placebo-controlled, acute pilot study with post-hoc subgroup analysis on 20 of the subjects. The study examined the effects of a single dose of a polyphenol-rich beverage (PRB), commercially marketed as "SoZo(®)", on serum anti-inflammatory and antioxidant markers. In addition, phytochemical analyses of PRB, and in vitro antioxidant capacity were also performed.
  
  
  RESULTS
  At 1 hour post-intake, serum values for 8-iso-PGF2-alpha and advanced oxidation protein products decreased significantly by 40% and 39%, respectively. Additionally, there was a trend toward decreased C-reactive protein, and increased nitric oxide levels. Both placebo and PRB treatment resulted in statistically significant increases in hydroxyl radical antioxidant capacity (HORAC) compared to baseline; PRB showed a higher percent change (55-75% versus 23-74% in placebo group), but the two groups did not differ significantly from each other.
  
  
  CONCLUSIONS
  PRB produced statistically significant changes in several blood biomarkers related to antioxidant/anti-inflammatory effects. Future studies are justified to verify results and test for cumulative effects of repeated intakes of PRB. The study demonstrates the potential utility of acute biomarker measurements for evaluating antioxidant/anti-inflammatory effects of natural products.
authors.affiliation: FutureCeuticals, Inc. Momence, IL 60954, USA. bnemzer@vandrunen.com
authors.forename: Boris V
authors.initials: BV
authors.lastname: Nemzer
authors.forename: Liliana C
authors.initials: LC
authors.lastname: Rodriguez
authors.forename: Linda
authors.initials: L
authors.lastname: Hammond
authors.forename: Robert
authors.initials: R
authors.lastname: Disilvestro
authors.forename: John M
authors.initials: JM
authors.lastname: Hunter
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Pietrzkowski
mesh_terms: D000872:Anthocyanins / Q000032:analysis; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D000975:Antioxidants / Q000032:analysis*; D001628:Beverages / Q000032:analysis*; D015415:Biomarkers / Q000097:blood; D002097:C-Reactive Protein / Q000032:analysis; D002726:Chlorogenic Acid / Q000032:analysis; D004044:Dietary Proteins / Q000097:blood; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000097:blood / Q000494:pharmacology; D004311:Double-Blind Method; D004610:Ellagic Acid / Q000032:analysis; D005260:Female; D006801:Humans; D017665:Hydroxyl Radical / Q000097:blood; D008297:Male; D008875:Middle Aged; D009569:Nitric Oxide / Q000097:blood; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010865:Pilot Projects; D059808:Polyphenols / Q000032:analysis / Q000494:pharmacology*; D044945:Proanthocyanidins / Q000032:analysis
chemical_list: D000872:Anthocyanins; D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D015415:Biomarkers; D004044:Dietary Proteins; D059808:Polyphenols; D044945:Proanthocyanidins; C013221:proanthocyanidin; D004610:Ellagic Acid; C075750:8-epi-prostaglandin F2alpha; D002726:Chlorogenic Acid; D009569:Nitric Oxide; D017665:Hydroxyl Radical; D002097:C-Reactive Protein; D015237:Dinoprost