title: [Anti-sense nucleic acid of CyclinD1 induces apoptosis of lung adenocarcinoma cancer cell A549].
abstract: To explore the potential of the anti-sense nucleic acid of CyclinD1 in lung cancer therapy, the expression vector containing the anti-sense nucleic acid of CyclinD1 was constructed and named pcDNA3.1-CyclinD1. The A549 cells were transfected with pcDNA3.1-CyclinD1 vectors. After being screened by G418, the stable expression positive clones were obtained. MTT method and flow cytometry technique were used to detect cell proliferation and apoptosis, respectively. The results showed the transfected cells exhibited significantly increased apoptosis and inhibited cell growth, compared with negative control and empty vector groups. To investigate the mechanism for anti-sense nucleic acid of CyclinD1 inducing A549 cells apoptosis, the expression levels of retinoblastoma protein (pRb), adenovirus E2 factor-1 (E2F-1), vascular endothelial growth factor (VEGF), matrix metalloproteinase (MMP)-2 and MMP-9 were detected by Western blot, and the results showed the expressions of these proteins were all decreased significantly in anti-sense nucleic acid of CyclinD transfected group, compared with those in negative control and empty vector groups. In a word, anti-sense nucleic acid of CyclinD1 induces the apoptosis of lung adenocarcinoma cancer cells, and the depressions of pRb, E2F-1, VEGF, MMP-2 and MMP-9 expressions may be the possible mechanism.
authors.affiliation: Department of Biochemistry and Molecular Biology, Binzhou Medical College, Yantai, China. lizunling@hotmail.com
authors.forename: Zun-Ling
authors.initials: ZL
authors.lastname: Li
authors.forename: Shu-Hong
authors.initials: SH
authors.lastname: Shao
authors.forename: Shu-Yang
authors.initials: SY
authors.lastname: Xie
authors.forename: Zhen
authors.initials: Z
authors.lastname: Yue
authors.forename: Ying
authors.initials: Y
authors.lastname: Ma
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D019938:Cyclin D1 / Q000235:genetics*; D016373:DNA, Antisense / Q000494:pharmacology*; D005822:Genetic Vectors; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D011995:Recombination, Genetic; D016160:Retinoblastoma Protein / Q000378:metabolism; D014162:Transfection; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: C530104:CCND1 protein, human; D016373:DNA, Antisense; D016160:Retinoblastoma Protein; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D019938:Cyclin D1; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9