title: Serum levels of Th17-related cytokines in Behcet disease patients after cataract surgery.
abstract: OBJECTIVE
  To investigate the profile of T-helper type 17 (Th17) cell-related cytokines (interleukin-23 [IL-23], IL-27, IL-17 and interferon-γ [IFN-γ]) in postoperative inflammation in patients with Behcet disease (BD) after cataract surgery.
  
  
  METHODS
  Serum was collected from seven BD patients with complicated cataract, and from nine controls with uncomplicated cataract, before cataract surgery, and again 1, 7, 30, and 90 days after surgery. In addition, aqueous humor was collected at commencement of surgery. The protein levels of IL-23, IL-27, IL-17, and IFN-γ in the serum and in the aqueous humor were measured by an enzyme-linked immunosorbent assay. A laser flare-cell photometer was used to quantify intraocular inflammation.
  
  
  RESULTS
  Serum IL-23, IL-27, and IFN-γ levels were significantly increased after cataract surgery in the BD versus the control patients. In the BD patients, serum levels of IFN-γ and IL-27 correlated strongly with aqueous flare values and cell counts. Remarkably, the levels of serum IL-27 were significantly associated with serum IFN-γ levels in BD patients (r=0.796; p=0.002).
  
  
  CONCLUSIONS
  Our data indicates that serum IFN-γ and IL-27 levels are significantly elevated in BD versus control patients and are strongly associated with post-operative intraocular inflammation.
authors.affiliation: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China.
authors.forename: Shuhong
authors.initials: S
authors.lastname: Jiang
authors.forename: Xialin
authors.initials: X
authors.lastname: Liu
authors.forename: Lixia
authors.initials: L
authors.lastname: Luo
authors.forename: Bo
authors.initials: B
authors.lastname: Qu
authors.forename: Xiangkun
authors.initials: X
authors.lastname: Huang
authors.forename: Ying
authors.initials: Y
authors.lastname: Lin
authors.forename: Shaobi
authors.initials: S
authors.lastname: Ye
authors.forename: Yizhi
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000328:Adult; D001082:Aqueous Humor / Q000737:chemistry / Q000276:immunology; D001528:Behcet Syndrome / Q000097:blood* / Q000150:complications / Q000276:immunology / Q000473:pathology / Q000601:surgery; D016022:Case-Control Studies; D002386:Cataract / Q000097:blood* / Q000276:immunology / Q000473:pathology; D002387:Cataract Extraction; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007249:Inflammation / Q000097:blood* / Q000150:complications / Q000276:immunology; D007371:Interferon-gamma / Q000096:biosynthesis*; D020381:Interleukin-17 / Q000096:biosynthesis*; D008297:Male; D011183:Postoperative Complications / Q000097:blood* / Q000276:immunology; D058504:Th17 Cells / Q000276:immunology
chemical_list: D020381:Interleukin-17; D007371:Interferon-gamma