title: GM-CSF increases cross-presentation and CD103 expression by mouse CD8⁺ spleen dendritic cells.
abstract: Resident CD8(+)  DCs perform several functions, including cross-presenting antigen and rapidly engulfing the Gram-positive intracellular pathogen Listeria monocytogenes. Little is known about how these functions of CD8(+)  DCs are modulated. Here, we show that granulocyte-macrophage CSF (GM-CSF), a cytokine that exists at low levels at steady state but is elevated during infection and inflammation, enhances cross-presentation and rapid uptake of L. monocytogenes by resident CD8(+)  DCs. This previously unrecognized functional enhancement of CD8(+)  DCs by GM-CSF was independent of promoting DC survival in vitro. Enhancement of these functions by GM-CSF was also marked by CD103 expression on CD8(+)  DCs that was strongly regulated by GM-CSF. Our findings not only identify GM-CSF as a key molecule regulating CD8(+)  DC function, but also as a factor responsible for functional heterogeneity of CD8(+)  DCs that is at least substantially demarcated by CD103 expression.
authors.affiliation: Immunology Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia. zhan@wehi.edu.au
authors.forename: Yifan
authors.initials: Y
authors.lastname: Zhan
authors.forename: Emma M
authors.initials: EM
authors.lastname: Carrington
authors.forename: Annemarie
authors.initials: A
authors.lastname: van Nieuwenhuijze
authors.forename: Sammy
authors.initials: S
authors.lastname: Bedoui
authors.forename: Shirley
authors.initials: S
authors.lastname: Seah
authors.forename: Yuekang
authors.initials: Y
authors.lastname: Xu
authors.forename: Nancy
authors.initials: N
authors.lastname: Wang
authors.forename: Justine D
authors.initials: JD
authors.lastname: Mintern
authors.forename: Jose A
authors.initials: JA
authors.lastname: Villadangos
authors.forename: Ian P
authors.initials: IP
authors.lastname: Wicks
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Lew
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D016827:CD8 Antigens / Q000096:biosynthesis; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D002478:Cells, Cultured; D045142:Cross-Priming / Q000235:genetics; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D005786:Gene Expression Regulation / Q000235:genetics; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D056747:Immunomodulation; D039001:Integrin alpha Chains / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D008089:Listeria monocytogenes / Q000276:immunology* / Q000472:pathogenicity; D008088:Listeriosis / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D013154:Spleen / Q000473:pathology
chemical_list: D000942:Antigens, Bacterial; D015703:Antigens, CD; D016827:CD8 Antigens; D039001:Integrin alpha Chains; C091367:alpha E integrins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor