title: Synthesis of a difunctional orthogonal coupler for the preparation of carbohydrate-functionalized sP(EO-stat-PO) hydrogels.
abstract: The synthesis and characterization of a new difunctional coupler (4) based on trimethylolpropane (TMP) are described. The coupler is used to connect biologically active N-acetylglucosamine (GlcNAc) on amino-reactive microtiter plates and on star-shaped poly[(ethylene oxide)-stat-(propylene oxide)] hydrogel layers in microtiter plates. The biological activity of immobilized carbohydrates is determined using an enzyme-linked lectin assay.
authors.affiliation: DWI an der RWTH Aachen e.V., and Institute of Technical and Macromolecular Chemistry, RWTH Aachen, Forckenbeckstraße 50, D-52056 Aachen, Germany.
authors.forename: Thorsten
authors.initials: T
authors.lastname: Anders
authors.forename: Kathrin
authors.initials: K
authors.lastname: Adamiak
authors.forename: Helmut
authors.initials: H
authors.lastname: Keul
authors.forename: Lothar
authors.initials: L
authors.lastname: Elling
authors.forename: Martin
authors.initials: M
authors.lastname: Möller
mesh_terms: D000117:Acetylglucosamine / Q000737:chemistry; D002241:Carbohydrates / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry*; D020100:Hydrogels / Q000138:chemical synthesis* / Q000737:chemistry; D037102:Lectins / Q000032:analysis; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D011092:Polyethylene Glycols / Q000737:chemistry*
chemical_list: D002241:Carbohydrates; D003432:Cross-Linking Reagents; D020100:Hydrogels; D037102:Lectins; D011092:Polyethylene Glycols; D000117:Acetylglucosamine