title: Homologous recombination assay for interstrand cross-link repair.
abstract: DNA interstrand cross-links (ICLs) covalently link both strands of the DNA duplex, impeding cellular processes like DNA replication. Homologous recombination (HR) is considered to be a major pathway for the repair of ICLs in mammalian cells as mutants for HR components are highly sensitive to DNA-damaging agents that cause ICLs. This chapter describes GFP assays to measure HR following site-specific ICL formation with psoralen through DNA triplex technology. This approach can be used to determine the genetic requirements for ICL-induced HR in relation to those involved in HR repair of other DNA lesions such as double-strand breaks.
authors.affiliation: Developmental Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10065, USA. nakanisk@mskcc.org
authors.forename: Koji
authors.initials: K
authors.lastname: Nakanishi
authors.forename: Francesca
authors.initials: F
authors.lastname: Cavallo
authors.forename: Erika
authors.initials: E
authors.lastname: Brunet
authors.forename: Maria
authors.initials: M
authors.lastname: Jasin
mesh_terms: D045744:Cell Line, Tumor; D004260:DNA Repair / Q000235:genetics*; D005434:Flow Cytometry; D006801:Humans; D009841:Oligonucleotides / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D011995:Recombination, Genetic / Q000235:genetics*
chemical_list: D009841:Oligonucleotides