title: Development of tinnitus in CBA/CaJ mice following sound exposure.
abstract: Tinnitus, the perception of a sound without an external acoustic source, is a complex perceptual phenomenon affecting the quality of life in 17% of the adult population. Despite its ubiquity and morbidity, the pathophysiology of tinnitus is a work in progress, and there is no generally accepted cure or treatment. Development of a reliable common animal model is crucial for tinnitus research and may advance this field. The goal of this study was to develop a tinnitus mouse model. Tinnitus was induced in an experimental group of mice by an exposure to a loud (116 dB sound pressure level (SPL)) narrow band noise (one octave, centered at 16 kHz) during 1 h under anesthesia. The tinnitus was then assessed behaviorally by measuring gap induced suppression of the acoustic startle reflex. We found that a vast majority of the sound-exposed mice (86%) developed behavioral signs of tinnitus. This was a complex, long lasting, and dynamic process. On the day following exposure, all mice demonstrated signs of acute tinnitus over the entire range of sound frequencies used for testing (10-31 kHz). However, 2-3 months later, a behavioral evidence of tinnitus was evident only at a narrow frequency range (20-31 kHz) representing a presumed chronic condition. Extracellular recordings confirmed a significantly higher rate of spontaneous activity in inferior colliculus neurons in sound-exposed compared to control mice. Surprisingly, unilateral sound exposure suppresses startle responses in mice and they remained suppressed even 3 months post-exposure, whereas auditory brainstem response thresholds were completely recovered during 2 months following exposure. In summary, behavioral evidence of tinnitus can be reliably developed in mice by sound exposure, and tinnitus induction can be assessed by quantifying prepulse inhibition of the acoustic startle reflex.
authors.affiliation: Department of Anatomy and Neurobiology, College of Medicine, Northeastern Ohio Universities, Rootstown, 44272, USA.
authors.forename: Ryan J
authors.initials: RJ
authors.lastname: Longenecker
authors.forename: Alexander V
authors.initials: AV
authors.lastname: Galazyuk
mesh_terms: D000818:Animals; D016057:Evoked Potentials, Auditory, Brain Stem; D051379:Mice; D008808:Mice, Inbred CBA; D009622:Noise / Q000009:adverse effects*; D012018:Reflex; D013216:Reflex, Startle; D014012:Tinnitus / Q000209:etiology* / Q000503:physiopathology