title: Synergistic responses of superficial chemistry and micro topography of titanium created by wire-type electric discharge machining.
abstract: Wire-type electric discharge machining has been applied to the manufacture of endosseous titanium implants as this computer associated technique allows extremely accurate complex sample shaping with an optimal micro textured surface during the processing. Since the titanium oxide layer is sensitively altered by each processing, the authors hypothesized that this technique also up-regulates biological responses through the synergistic effects of the superficial chemistry and micro topography. To evaluate the respective in vitro cellular responses on the superficial chemistry and micro topography of titanium surface processed by wire-type electric discharge, we used titanium-coated epoxy resin replica of the surface. An oxide layer on the titanium surface processed by wire-type electric discharge activated the initial responses of osteoblastic cells through an integrin-mediated mechanism. Since the mRNA expression of ALP on those replicas was up-regulated compared to smooth titanium samples, the micro topography of a titanium surface processed by wire-type electric discharge promotes the osteogenic potential of cells. The synergistic response of the superficial chemistry and micro topography of titanium processed by wire-type electric discharge was demonstrated in this study.
authors.affiliation: Department of Oral Biomaterials and Technology, School of Dentistry, Showa University, Tokyo, Japan.
authors.forename: Yu
authors.initials: Y
authors.lastname: Kataoka
authors.forename: Yukimichi
authors.initials: Y
authors.lastname: Tamaki
authors.forename: Takashi
authors.initials: T
authors.lastname: Miyazaki
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion; D002460:Cell Line; D004357:Drug Synergism; D004853:Epoxy Resins / Q000737:chemistry; D008422:Materials Testing; D051379:Mice; D008855:Microscopy, Electron, Scanning / Q000379:methods; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D010006:Osteoblasts / Q000201:enzymology* / Q000648:ultrastructure; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*; D015854:Up-Regulation
chemical_list: D001672:Biocompatible Materials; D004853:Epoxy Resins; C009495:titanium dioxide; D014025:Titanium; D000469:Alkaline Phosphatase