title: Identification of robust hypoxia biomarker candidates from fin of medaka (Oryzias latipes).
abstract: Aquatic hypoxia caused by organic pollution and eutrophication is a pressing worldwide water pollution problem. Better methods for monitoring oxygen levels are needed to assist efforts to maintain and protect the health of natural aquatic environments. In this project, we used a Japanese ricefish (medaka, Oryzias latipes) 8K oligonucleotide array as a platform to identify potential hypoxic biomarkers in different organs (fin, gill, liver and brain) upon exposure to hypoxia. The microarray results were validated by qRT-PCR employing a subset of candidate biomarkers. Interestingly, the largest number and most significant of hypoxia responding array features were detected in hypoxia exposed fin tissues. We identified 173 array features that exhibited a significant response (over 2 fold change in expression) upon exposure to hypoxic conditions and validated a subset of these by quantitative RT-PCR. These gene targets were subjected to annotation and gene ontology mining. Positively identifiable gene targets that may be useful for development of a rapid and accurate biomarker test using fin clips are discussed in relation to previous reports on hypoxia responsive genes.
authors.affiliation: Department of Chemistry and Biochemistry, Molecular Biosciences Research Group, 419 Centennial Hall, Texas State University, 601 University Drive, San Marcos, TX 78666, USA
authors.forename: Ziping
authors.initials: Z
authors.lastname: Zhang
authors.forename: Melissa C
authors.initials: MC
authors.lastname: Wells
authors.forename: Mikki G
authors.initials: MG
authors.lastname: Boswell
authors.forename: Ion
authors.initials: I
authors.lastname: Beldorth
authors.forename: Lyndsey M
authors.initials: LM
authors.lastname: Kirk
authors.forename: Yilei
authors.initials: Y
authors.lastname: Wang
authors.forename: Shulong
authors.initials: S
authors.lastname: Wang
authors.forename: Markita
authors.initials: M
authors.lastname: Savage
authors.forename: Ronald B
authors.initials: RB
authors.lastname: Walter
authors.forename: Rachell E
authors.initials: RE
authors.lastname: Booth
mesh_terms: D058500:Animal Fins / Q000378:metabolism* / Q000502:physiology; D000818:Animals; D015415:Biomarkers; D001921:Brain / Q000378:metabolism / Q000502:physiology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D005880:Gills / Q000378:metabolism / Q000502:physiology; D000860:Hypoxia / Q000378:metabolism*; D008099:Liver / Q000378:metabolism / Q000502:physiology; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D009990:Oryzias / Q000235:genetics / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D015415:Biomarkers