title: Effects of the Crotalus durissus terrificus snake venom on hepatic metabolism and oxidative stress.
abstract: Snake venoms present different action mechanisms because of their complex composition, represented mainly by toxins and enzymes. This work aimed to investigate the effects of the Crotalus durissus terrificus(Cdt) venom in the liver. Wistar rats were inoculated intraperitoneally with saline (control) or Cdt venom. After 3, 4, or 6 h, the following parameters were analyzed: (a) hepatic function, (b) oxidative stress parameters, and (c) the metabolism of alanine in the isolated perfused liver. Plasma activities of alanine aminotransferase and aspartate aminotransferase and hepatic glutathione S-transferase and catalase presented significant elevation in rats inoculated with 300 μg ⋅ kg(-1) Cdt venom. Liver lipoperoxidation was enormously increased by venom doses of 100, 200, and 300 μg ⋅kg(-1) , whereas glutathione S-transferase was not changed. Perfused livers from rats inoculated with 1500 μg ⋅kg(-1) venom showed increased production of lactate, pyruvate, and ammonia when alanine was the metabolic substrate. These results demonstrate that the Cdt venom can produce several changes in hepatocytes. The causes of the changes are possibly related to the disequilibrium in the redox homeostasis but also to specific needs of the poisoned organism, for example, an increased supply of lactate and pyruvate in response to an increased activity of the Cori cycle.
authors.affiliation: Department of Pharmacology, Federal University of Paraná, Biological Science Sector, Jardim das Américas, Curitiba, Paraná, Brazil.
authors.forename: Jonas Golart
authors.initials: JG
authors.lastname: da Silva
authors.forename: Bruna
authors.initials: B
authors.lastname: da Silva Soley
authors.forename: Vanessa
authors.initials: V
authors.lastname: Gris
authors.forename: Amanda
authors.initials: A
authors.lastname: do Rocio Andrade Pires
authors.forename: Silvia Maria Suter Correia
authors.initials: SM
authors.lastname: Caderia
authors.forename: Gabrielle Jackin
authors.initials: GJ
authors.lastname: Eler
authors.forename: Aparecida Pinto Munhos
authors.initials: AP
authors.lastname: Hermoso
authors.forename: Adelar
authors.initials: A
authors.lastname: Bracht
authors.forename: Paulo Roberto
authors.initials: PR
authors.lastname: Dalsenter
authors.forename: Alexandra
authors.initials: A
authors.lastname: Acco
mesh_terms: D000409:Alanine / Q000378:metabolism; D000410:Alanine Transaminase / Q000097:blood; D000641:Ammonia / Q000378:metabolism; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D003435:Crotalid Venoms / Q000633:toxicity*; D017839:Crotalus / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D019289:Pyruvic Acid / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
chemical_list: D003435:Crotalid Venoms; D019344:Lactic Acid; D000641:Ammonia; D019289:Pyruvic Acid; D005982:Glutathione Transferase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D005978:Glutathione; D000409:Alanine