title: Cowpox virus induces interleukin-10 both in vitro and in vivo.
abstract: Cowpox virus infection induces interleukin-10 (IL-10) production from mouse bone marrow-derived dendritic cells (BMDCs) or cells of the mouse macrophage line (RAW264.7) at about 1800 pg/ml, whereas infections with vaccinia virus (strains WR or MVA) induced much less IL-10. Similarly, in vivo, IL-10 levels in bronchoalveolar lavage fluids of mice infected with cowpox virus were significantly higher than those after vaccinia virus infection. However, after intranasal cowpox virus infection, although dendritic and T-cell accumulations in the lungs of IL-10 deficient mice were greater than those in wild-type mice, weight-loss and viral burdens were not significantly different. IL-10 deficient mice were more susceptible than wild-type mice to re-infection with cowpox virus even though titers of neutralizing antibodies and virus-specific CD8 T cells were similar between IL-10 deficient and wild-type mice. Greater bronchopneumonia in IL-10 deficient mice than wild-type mice suggests that IL-10 contributes to the suppression of immunopathology in the lungs.
authors.affiliation: Department of Molecular Genetics and Microbiology, Box 3020, Duke University Medical Center, Durham, NC 27710, USA.
authors.forename: April H
authors.initials: AH
authors.lastname: Spesock
authors.forename: Brice E
authors.initials: BE
authors.lastname: Barefoot
authors.forename: Caroline A
authors.initials: CA
authors.lastname: Ray
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Kenan
authors.forename: Michael D
authors.initials: MD
authors.lastname: Gunn
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Ramsburg
authors.forename: David J
authors.initials: DJ
authors.lastname: Pickup
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism; D002460:Cell Line; D015605:Cowpox / Q000276:immunology*; D015608:Cowpox virus / Q000502:physiology*; D003713:Dendritic Cells / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology; D016753:Interleukin-10 / Q000235:genetics / Q000378:metabolism*; D008168:Lung / Q000166:cytology; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D009154:Mutation; D013601:T-Lymphocytes; D015431:Weight Loss
chemical_list: D016753:Interleukin-10