title: [Content of energy substrates in fibers of postural muscle soleus and its antagonist in the condition of support deprivation].
abstract: The investigation had the purpose to measure energy substrates in different fibers of 3- and 14-d suspended postural m. soleus and its main antagonist - anterior tibial muscle in rats. Hindlimb suspension served as a model of gravitational unloading. After suspension, left m. soleus or anterior tibial muscle was extracted. Serial cross-sections were subject to histochemical staining by Oil Red O (Sigma) for determination of intracellular triglycerides or by Schiff's reagent and iododic acid (Sigma-ALDRICH) for determination of glycogen and simultaneous immunohistochemical fibers typing by antibodies to heavy types I and II myosin chains (NCL-MHCs and NCL-MHCf, Novocastra). Analysis of intracellular energy substrates showed that both types of fibers in the anterior tibial muscle had accumulated triglycerides by week-2 of suspension. At the same time, glycogen level in equally slow and fast fibers of m. soleus and fast fibers anterior tibial muscle decreased substantially after 3 days of suspension but returned to baseline value by day 14. These changes are likely to be linked with altered activity of the muscles and hormonal factor.
authors.forename: M G
authors.initials: MG
authors.lastname: Tavitova
authors.forename: N M
authors.initials: NM
authors.lastname: Fokina
authors.forename: B S
authors.initials: BS
authors.lastname: Shenkman
mesh_terms: D000818:Animals; D004734:Energy Metabolism / Q000502:physiology*; D005500:Follow-Up Studies; D006003:Glycogen / Q000378:metabolism*; D019417:Hindlimb Suspension; D007150:Immunohistochemistry; D008297:Male; D008856:Microscopy, Fluorescence; D018485:Muscle Fibers, Skeletal / Q000166:cytology / Q000378:metabolism; D018482:Muscle, Skeletal / Q000166:cytology / Q000378:metabolism*; D018995:Myosin Heavy Chains / Q000378:metabolism*; D011187:Posture / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
chemical_list: D006003:Glycogen; D018995:Myosin Heavy Chains