title: Cloning and expression of a β-galactosidase gene of Bacillus circulans.
abstract: A gene of β-galactosidase from Bacillus circulans ATCC 31382 was cloned and sequenced on the basis of N-terminal and internal peptide sequences isolated from a commercial enzyme preparation, Biolacta(®). Using the cloned gene, recombinant β-galactosidase and its deletion mutants were overexpressed as His-tagged proteins in Escherichia coli cells and the enzymes expressed were characterized.
authors.affiliation: Graduate School of Natural Science and Technology, Okayama University, Kita-ku, Okayama, Japan.
authors.forename: Jingyuan
authors.initials: J
authors.lastname: Song
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Imanaka
authors.forename: Koreyoshi
authors.initials: K
authors.lastname: Imamura
authors.forename: Masashi
authors.initials: M
authors.lastname: Minoda
authors.forename: Toru
authors.initials: T
authors.lastname: Katase
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Hoshi
authors.forename: Shotaro
authors.initials: S
authors.lastname: Yamaguchi
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Nakanishi
mesh_terms: D020816:Amino Acid Motifs; D001407:Bacillus / Q000201:enzymology / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D004926:Escherichia coli; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism*; D007785:Lactose / Q000378:metabolism; D010957:Plasmids; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D014169:Transformation, Bacterial; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D007527:Isoenzymes; D011994:Recombinant Proteins; D001616:beta-Galactosidase; D007785:Lactose