title: Inhibitory effects of TRPV1 blocker on UV-induced responses in the hairless mice.
abstract: The transient receptor potential vanilloid 1 (TRPV1) channel can be activated by vanilloids, exposure to ultraviolet (UV) irradiation, heat, or protons, and conditions that occur during tissue injury. In the present study, we investigated whether or not TRPV1-specific blocker, 5'-iodoresiniferatoxin (I-RTX), can reduce UV-induced matrix metalloproteinases (MMPs), pro-inflammatory cytokines, cyclooxygenase (COX)-2, and p53 expression in the skin of hairless mice. Our results showed that I-RTX inhibited UV-induced skin thickening, as measured by a caliper, or in hematoxylin and eosin (H&E)-stained sections. UV-induced mRNA and protein expression of MMP-13, MMP-9, MMP-3, and MMP-2 was significantly reduced by I-RTX. We also observed the inhibitory effects of I-RTX on UV-induced mRNA expression of the pro-inflammatory cytokines, interleukin (IL)-1β, IL-2, IL-4, and tumor necrosis factor-α. UV-induced COX-2 and p53 protein expression was also significantly decreased by I-RTX. From the above results, we suggest that TRPV1-specific blocker, I-RTX, could prevent UV-induced skin responses, and provide new insight into development of effective therapeutic methods for photoaging.
authors.affiliation: Department of Dermatology, Seoul National University Hospital,Yeongeon-Dong, Chongno-Gu, Korea.
authors.forename: Young Mee
authors.initials: YM
authors.lastname: Lee
authors.forename: So Min
authors.initials: SM
authors.lastname: Kang
authors.forename: Se Rah
authors.initials: SR
authors.lastname: Lee
authors.forename: Kyung Hwan
authors.initials: KH
authors.lastname: Kong
authors.forename: Jeong Yoon
authors.initials: JY
authors.lastname: Lee
authors.forename: Eun Ju
authors.initials: EJ
authors.lastname: Kim
authors.forename: Jin Ho
authors.initials: JH
authors.lastname: Chung
mesh_terms: D000375:Aging / Q000187:drug effects / Q000528:radiation effects; D000818:Animals; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D004224:Diterpenes / Q000008:administration & dosage* / Q000009:adverse effects; D005786:Gene Expression Regulation / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism; D020782:Matrix Metalloproteinases / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008812:Mice, Hairless; D012867:Skin / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects*; D050916:TRPV Cation Channels / Q000037:antagonists & inhibitors; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D016207:Cytokines; D004224:Diterpenes; D018836:Inflammation Mediators; D050916:TRPV Cation Channels; C494482:TRPV1 protein, human; D016159:Tumor Suppressor Protein p53; C468130:iodoresiniferatoxin; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2; D020782:Matrix Metalloproteinases