title: Microarray expression profiling of Spodoptera litura in response to oxidative stress.
abstract: To examine the expression profile of oxidative stress responsive genes in Spodoptera litura, we constructed a cDNA library from S. litura injected with hydrogen peroxide (H(2)O(2)). Using a microarray chip composed of 2,964 cDNAs, we screened gene expression at 1, 3, 5, 7, and 9 h post H(2)O(2) injection. Data were clustered into 15 groups of genes that behave similarly across each time course. Seventy-three genes were identified as being at least twofold up- or downregulated after treatment with H(2)O(2) in S. litura. We constructed expressed sequence tags (ESTs) for genes that changed at least twofold after treatment with H(2)O(2) . The functional classification of these ESTs based on Gene Ontology showed that the ESTs are rich in genes involved in oxidoreductase activity (5.7%), defense (14.3%), cellular process (22.9%), and development (17.1%).
authors.affiliation: Department of Agricultural Biology, National Academy of Agricultural Science, RDA, Suwon, South Korea.
authors.forename: Eun-Young
authors.initials: EY
authors.lastname: Yun
authors.forename: Jae-Sam
authors.initials: JS
authors.lastname: Hwang
authors.forename: Young-Il
authors.initials: YI
authors.lastname: Yoon
authors.forename: Mi-Young
authors.initials: MY
authors.lastname: Ahn
authors.forename: Nam-Jung
authors.initials: NJ
authors.lastname: Kim
authors.forename: O-Yu
authors.initials: OY
authors.lastname: Kwon
authors.forename: Won-Jae
authors.initials: WJ
authors.lastname: Lee
authors.forename: Tae-Won
authors.initials: TW
authors.lastname: Goo
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D057225:Data Mining; D020869:Gene Expression Profiling; D017344:Genes, Insect; D006861:Hydrogen Peroxide / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D018384:Oxidative Stress; D018411:Spodoptera / Q000378:metabolism*
chemical_list: D006861:Hydrogen Peroxide