title: Relationship between articular cartilage damage and subchondral bone properties and meniscal ossification in the Dunkin Hartley guinea pig model of osteoarthritis.
abstract: OBJECTIVE
  To describe the age-related changes of articular cartilage, subchondral bone morphology, and stiffness. Furthermore, to investigate whether subchondral bone histological and mechanical properties and meniscal histological properties are related to articular cartilage damage in the Dunkin Hartley guinea pig model of osteoarthritis (OA).
  
  
  METHODS
  Forty male Dunkin Hartley guinea pigs aged 2, 6, 9, and 12 months were studied. The right stifle joints and the left menisci were embedded undecalcified and the tibial articular cartilage and subchondral bone and the menisci were examined using histology. The stiffness of the left tibial subchondral bone was determined with indentation testing.
  
  
  RESULTS
  The Osteoarthritis Research Society International (OARSI) grade of the osteoarthritic cartilage lesions of the medial (p < 0.001) and lateral (p < 0.001) condyle and the ossification of the medial (p < 0.001) and lateral (p < 0.001) meniscus increased significantly with age and was significantly more pronounced at the medial condyle than at the lateral condyle. The grade of the osteoarthritic cartilage lesions was significantly correlated (r  =  0.78, p < 0.001) with the meniscal ossification, weakly correlated (r  =  0.34, p < 0.007) with the subchondral bone plate thickness, and not correlated with the subchondral bone density (r  =  -0.010, p  =  0.94) and the subchondral bone stiffness (r  =  -0.13, p  =  0.30).
  
  
  CONCLUSIONS
  The meniscal ossification observed in Dunkin Hartley guinea pigs may play an important role in the pathogenesis of OA in these animals.
authors.affiliation: Department of Connective Tissue Biology, Institute of Anatomy, University of Aarhus, Aarhus, Denmark. jesper@jst.ana.au.dk
authors.forename: J S
authors.initials: JS
authors.lastname: Thomsen
authors.forename: T S
authors.initials: TS
authors.lastname: Straarup
authors.forename: C C
authors.initials: CC
authors.lastname: Danielsen
authors.forename: H
authors.initials: H
authors.lastname: Oxlund
authors.forename: A
authors.initials: A
authors.lastname: Brüel
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000473:pathology*; D004195:Disease Models, Animal; D006168:Guinea Pigs; D007596:Joints / Q000473:pathology; D008297:Male; D008592:Menisci, Tibial / Q000473:pathology*; D009999:Ossification, Heterotopic / Q000473:pathology*; D010003:Osteoarthritis / Q000473:pathology*