title: Expression of HOXB genes is significantly different in acute myeloid leukemia with a partial tandem duplication of MLL vs. a MLL translocation: a cross-laboratory study.
abstract: In acute myeloid leukemia (AML), the mixed lineage leukemia (MLL) gene may be rearranged to generate a partial tandem duplication (PTD), or fused to partner genes through a chromosomal translocation (tMLL). In this study, we first explored the differentially expressed genes between MLL-PTD and tMLL using gene expression profiling of our cohort (15 MLL-PTD and 10 tMLL) and one published data set. The top 250 probes were chosen from each set, resulting in 29 common probes (21 unique genes) to both sets. The selected genes include four HOXB genes, HOXB2, B3, B5, and B6. The expression values of these HOXB genes significantly differ between MLL-PTD and tMLL cases. Clustering and classification analyses were thoroughly conducted to support our gene selection results. Second, as MLL-PTD, FLT3-ITD, and NPM1 mutations are identified in AML with normal karyotypes, we briefly studied their impact on the HOXB genes. Another contribution of this study is to demonstrate that using public data from other studies enriches samples for analysis and yields more conclusive results.
authors.affiliation: Division of Pediatric Hematology-Oncology, Mackay Memorial Hospital, Taipei, Taiwan.
authors.forename: Hsi-Che
authors.initials: HC
authors.lastname: Liu
authors.forename: Lee-Yung
authors.initials: LY
authors.lastname: Shih
authors.forename: Mei-Ju
authors.initials: MJ
authors.lastname: May Chen
authors.forename: Chien-Chih
authors.initials: CC
authors.lastname: Wang
authors.forename: Ting-Chi
authors.initials: TC
authors.lastname: Yeh
authors.forename: Tung-Huei
authors.initials: TH
authors.lastname: Lin
authors.forename: Chien-Yu
authors.initials: CY
authors.lastname: Chen
authors.forename: Chih-Jen
authors.initials: CJ
authors.lastname: Lin
authors.forename: Der-Cherng
authors.initials: DC
authors.lastname: Liang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016000:Cluster Analysis; D005260:Female; D020440:Gene Duplication; D015972:Gene Expression Regulation, Neoplastic; D011495:Histone-Lysine N-Methyltransferase; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D051788:Myeloid-Lymphoid Leukemia Protein / Q000235:genetics*; D000090243:Nucleophosmin; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014178:Translocation, Genetic
chemical_list: C086061:HOXB2 protein, human; C489126:HOXB5 protein, human; C093252:HOXB6 protein, human; D018398:Homeodomain Proteins; C542921:HoxB3 protein, human; C497422:KMT2A protein, human; C000716967:NPM1 protein, human; D014157:Transcription Factors; D000090243:Nucleophosmin; D051788:Myeloid-Lymphoid Leukemia Protein; D011495:Histone-Lysine N-Methyltransferase