title: A rapid and environmental friendly determination of the dithiocarbamate metabolites ethylenethiourea and propylenethiourea in fruit and vegetables by ultra high performance liquid chromatography tandem mass spectrometry.
abstract: Previous published methods for the analysis of ETU and PTU are time-consuming and furthermore use dichloromethane (DCM) for extraction or clean-up. This study details the development and validation of a rapid method that combines a simple extraction step with UHPLC-ESI(+)-MS/MS. This is the first application of UHPLC-MS/MS to analyse these compounds. Besides that, we replaced DCM with a more environmental-friendly solvent. The analytical performance was evaluated with the analysis of spiked celery samples at 50 μg kg(-1) (LOQ) and 300 μg kg(-1). The recoveries were between 65% and 90% for ETU and between 71% and 127% for PTU with RSDs in repeatability and reproducibility conditions below 10% for ETU. This method is rapid (a chromatographic run time of 2 min) and can easily be performed (no laborious clean-up). The presented method is environmental friendly with significant reduction in solvent consumption.
authors.affiliation: Food, Medicines and Consumer Safety Department, Scientific Institute of Public Health, Rue Juliette Wytsman 14, 1050 Ixelles, Belgium.
authors.forename: Aurore
authors.initials: A
authors.lastname: Bonnechère
authors.forename: Vincent
authors.initials: V
authors.lastname: Hanot
authors.forename: Joris
authors.initials: J
authors.lastname: Van Loco
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004355:Drug Stability; D005031:Ethylenethiourea / Q000032:analysis* / Q000378:metabolism; D005638:Fruit / Q000737:chemistry*; D005659:Fungicides, Industrial / Q000032:analysis; D016014:Linear Models; D010573:Pesticide Residues / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods; D013859:Thiocarbamates / Q000032:analysis* / Q000378:metabolism; D013890:Thiourea / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism; D014675:Vegetables / Q000737:chemistry*
chemical_list: D005659:Fungicides, Industrial; D010573:Pesticide Residues; D013859:Thiocarbamates; C035039:propylenethiourea; D005031:Ethylenethiourea; D013890:Thiourea