title: The effects of rose bengal- and erythrosine-mediated photodynamic therapy on Candida albicans.
abstract: The aim of this study was to evaluate the effects of photodynamic therapy (PDT) using rose bengal or erythrosine with light emitting diode (LED) on Candida albicans planktonic cultures and biofilms. Seven C. albicans clinical strains and one standard strain (ATCC 18804) were used. Planktonic cultures and biofilms of each C. albicans strain were submitted to the following experimental conditions: (a) treatment with rose bengal and LED (RB+L+); (b) treatment with erythrosine and LED (E+L+); and (c) control group, without LED irradiation or photosensitiser treatment (P-L-). After irradiation of the planktonic cultures and biofilms, the cultures were seeded onto Sabouraud dextrose agar (37 °C at 48 h) for counting of colony-forming units (CFU ml(-1) ) followed by posterior anova and Tukey's test analyses (P < 0.05). The biofilms were analysed using scanning electron microscopy (SEM). The results revealed a significant reduction of planktonic cultures (3.45 log(10) and 1.97 log(10) ) and of biofilms (<1 log(10) ) for cultures that were subjected to PDT mediated using either erythrosine or rose bengal, respectively. The SEM data revealed that the PDT was effective in reducing and destroying of C. albicans blastoconidia and hyphae. The results show that erythrosine- and rose bengal-mediated PDT with LED irradiation is effective in treating C. albicans.
authors.affiliation: Department of Biosciences and Oral Diagnosis, School of Dentistry of São José dos Campos, UNESP-University Estadual Paulista, São José dos Campos, SP, Brazil. carol_biolog@yahoo.com.br
authors.forename: Anna Carolina Borges Pereira
authors.initials: AC
authors.lastname: Costa
authors.forename: Vanessa Maria Campos
authors.initials: VM
authors.lastname: Rasteiro
authors.forename: Cristiane Aparecida
authors.initials: CA
authors.lastname: Pereira
authors.forename: Rodnei Dennis
authors.initials: RD
authors.lastname: Rossoni
authors.forename: Juliana Campos
authors.initials: JC
authors.lastname: Junqueira
authors.forename: Antonio Olavo Cardoso
authors.initials: AO
authors.lastname: Jorge
mesh_terms: D000704:Analysis of Variance; D000935:Antifungal Agents / Q000494:pharmacology; D018441:Biofilms; D002176:Candida albicans / Q000187:drug effects* / Q000254:growth & development; D002177:Candidiasis / Q000188:drug therapy / Q000382:microbiology; D015169:Colony Count, Microbial; D004923:Erythrosine / Q000494:pharmacology*; D025301:Hyphae / Q000187:drug effects / Q000648:ultrastructure; D008027:Light; D008855:Microscopy, Electron, Scanning; D010778:Photochemotherapy; D012395:Rose Bengal / Q000494:pharmacology*; D013172:Spores, Fungal / Q000187:drug effects / Q000648:ultrastructure
chemical_list: D000935:Antifungal Agents; D012395:Rose Bengal; D004923:Erythrosine