title: Förster resonance energy transfer demonstrates a flavonoid metabolon in living plant cells that displays competitive interactions between enzymes.
abstract: We have used Förster resonance energy transfer detected by fluorescence lifetime imaging microscopy (FLIM-FRET) to provide the first evidence from living plants cells for the existence of a flavonoid metabolon. The distribution of flux within this system may be regulated by the direct competition of enzymes that catalyze key branch-point reactions, flavonol synthase 1 and dihydroflavonol 4-reductase, for association with the entry-point enzyme, chalcone synthase. Because the flavonoid enzymes were likely recruited from pathways of primary metabolism, our findings suggest a new general working model for the regulation of dynamic pathways in their native cellular context.
authors.affiliation: The Department of Biological Sciences, Virginia Tech, Blacksburg, VA 24061, United States.
authors.forename: Kevin C
authors.initials: KC
authors.lastname: Crosby
authors.forename: Anna
authors.initials: A
authors.lastname: Pietraszewska-Bogiel
authors.forename: Theodorus W J
authors.initials: TW
authors.lastname: Gadella
authors.forename: Brenda S J
authors.initials: BS
authors.lastname: Winkel
mesh_terms: D017360:Arabidopsis / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism; D005419:Flavonoids / Q000737:chemistry* / Q000378:metabolism*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods
chemical_list: D029681:Arabidopsis Proteins; D005419:Flavonoids