title: Cell plate restricted association of DRP1A and PIN proteins is required for cell polarity establishment in Arabidopsis.
abstract: The polarized transport of the phytohormone auxin [1], which is crucial for the regulation of different stages of plant development [2, 3], depends on the asymmetric plasma membrane distribution of the PIN-FORMED (PIN) auxin efflux carriers [4, 5]. The PIN polar localization results from clathrin-mediated endocytosis (CME) from the plasma membrane and subsequent polar recycling [6]. The Arabidopsis genome encodes two groups of dynamin-related proteins (DRPs) that show homology to mammalian dynamin-a protein required for fission of endocytic vesicles during CME [7, 8]. Here we show by coimmunoprecipitation (coIP), bimolecular fluorescence complementation (BiFC), and Förster resonance energy transfer (FRET) that members of the DRP1 group closely associate with PIN proteins at the cell plate. Localization and phenotypic analysis of novel drp1 mutants revealed a requirement for DRP1 function in correct PIN distribution and in auxin-mediated development. We propose that rapid and specific internalization of PIN proteins mediated by the DRP1 proteins and the associated CME machinery from the cell plate membranes during cytokinesis is an important mechanism for proper polar PIN positioning in interphase cells.
authors.affiliation: Department of Plant Systems Biology, VIB, and Department of Plant Biotechnology and Genetics, Ghent University, 9052 Gent, Belgium, Germany.
authors.forename: Jozef
authors.initials: J
authors.lastname: Mravec
authors.forename: Jan
authors.initials: J
authors.lastname: Petrášek
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.forename: Sjef
authors.initials: S
authors.lastname: Boeren
authors.forename: Rumyana
authors.initials: R
authors.lastname: Karlova
authors.forename: Saeko
authors.initials: S
authors.lastname: Kitakura
authors.forename: Markéta
authors.initials: M
authors.lastname: Pařezová
authors.forename: Satoshi
authors.initials: S
authors.lastname: Naramoto
authors.forename: Tomasz
authors.initials: T
authors.lastname: Nodzyński
authors.forename: Pankaj
authors.initials: P
authors.lastname: Dhonukshe
authors.forename: Sebastian Y
authors.initials: SY
authors.lastname: Bednarek
authors.forename: Eva
authors.initials: E
authors.lastname: Zažímalová
authors.forename: Sacco
authors.initials: S
authors.lastname: de Vries
authors.forename: Jiří
authors.initials: J
authors.lastname: Friml
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000166:cytology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D016764:Cell Polarity; D034281:Dynamins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D018745:Genome, Plant; D049452:Green Fluorescent Proteins / Q000235:genetics; D047468:Immunoprecipitation; D026901:Membrane Transport Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data; D013050:Spectrometry, Fluorescence
chemical_list: D029681:Arabidopsis Proteins; D026901:Membrane Transport Proteins; C116363:PIN1 protein, Arabidopsis; D049452:Green Fluorescent Proteins; C475162:ADL1A protein, Arabidopsis; D034281:Dynamins