title: Effects of dicalcium silicate coating ionic dissolution products on human mesenchymal stem-cell proliferation and osteogenic differentiation.
abstract: This study investigated the effects of ionic dissolution products released from dicalcium silicate (DS) coatings on human mesenchymal stem cells (hMSC), cultured in the presence or absence of the dissolution products, with or without osteogenic supplements (OS). DS(+) medium promoted cell proliferation during the first 4 days, but then inhibited proliferation. DS(+)OS(-) medium increased alkaline phosphatase (ALP) activity on day 14, and upregulated runt-related transcription factor 2 and osteonectin mRNA on days 7 and 14, respectively. The addition of osteogenic supplements (DS(+)OS(+)) led to a significant increase in ALP activity from days 7 to 21, upregulation of osteogenic markers on day 14, and formation of more mineralized nodules on day 28. The results demonstrated that the ionic dissolution products from DS coating alone can partly induce osteogenic differentiation of hMSC, and that the addition of osteogenic supplements further enhances osteoblast-specific gene expression and mineralization in hMSC.
authors.affiliation: Department of Orthopaedics, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China.
authors.forename: H-W
authors.initials: HW
authors.lastname: Li
authors.forename: J-Y
authors.initials: JY
authors.lastname: Sun
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D000880:Anthraquinones / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D018786:Bone Substitutes / Q000737:chemistry / Q000494:pharmacology*; D001842:Bone and Bones / Q000166:cytology / Q000378:metabolism; D017610:Calcium Compounds / Q000737:chemistry / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology*; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics / Q000378:metabolism; D003470:Culture Media; D005434:Flow Cytometry; D015870:Gene Expression; D006801:Humans; D007477:Ions / Q000737:chemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008875:Middle Aged; D010012:Osteogenesis / Q000187:drug effects*; D015676:Osteonectin / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017640:Silicates / Q000737:chemistry / Q000494:pharmacology*; D012995:Solubility
chemical_list: D000880:Anthraquinones; D015415:Biomarkers; D018786:Bone Substitutes; D017610:Calcium Compounds; D020099:Coated Materials, Biocompatible; D050659:Core Binding Factor Alpha 1 Subunit; D003470:Culture Media; D007477:Ions; D015676:Osteonectin; D012333:RNA, Messenger; C493709:RUNX2 protein, human; D017640:Silicates; C013481:dicalcium silicate; C004468:Alizarin Red S; D000469:Alkaline Phosphatase