title: Diagnostic consequences of cytomegalovirus glycoprotein B polymorphisms.
abstract: Failure of a cytomegalovirus (CMV) real-time PCR assay targeting glycoprotein B (gB) was investigated. A multiplex assay targeting gB and immediate-early 2 (IE2) genes showed discordant results (gB negative and IE positive or a >10-fold-higher viral load with IE primers) in saliva from 14.6% of CMV-infected newborns. Sequencing revealed 3 patterns of gB variations.
authors.affiliation: University of Alabama at Birmingham, Children's Hospital, CHB 150, 1600 6th Ave. S., Birmingham, AL 35233, USA. zdnovak@peds.uab.edu
authors.forename: Zdenek
authors.initials: Z
authors.lastname: Novak
authors.forename: Nazma
authors.initials: N
authors.lastname: Chowdhury
authors.forename: Shannon A
authors.initials: SA
authors.lastname: Ross
authors.forename: Sunil K
authors.initials: SK
authors.lastname: Pati
authors.forename: Karen
authors.initials: K
authors.lastname: Fowler
authors.forename: Suresh B
authors.initials: SB
authors.lastname: Boppana
mesh_terms: D003587:Cytomegalovirus / Q000235:genetics* / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000175:diagnosis* / Q000821:virology; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics; D007223:Infant; D007231:Infant, Newborn; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D011110:Polymorphism, Genetic; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012463:Saliva / Q000821:virology; D012680:Sensitivity and Specificity; D015534:Trans-Activators / Q000235:genetics; D014759:Viral Envelope Proteins / Q000235:genetics*; D014773:Virology / Q000379:methods
chemical_list: C069186:IE2 protein, Cytomegalovirus; D017874:Immediate-Early Proteins; D015534:Trans-Activators; D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus