title: Lack of association of the genotype in the GNAS Fok I polymorphism and prostate cancer.
abstract: BACKGROUND
  G proteins are ubiquitously expressed signal transduction proteins playing a key role in multiple signal transduction pathways. The Gαs subunit has been considered as an apoptosis factor. In this study the role of GNAS T393C genotypes of the GNAS gene encoding Gαs was analyzed for its influence on the development and progression of prostate cancer.
  
  
  METHODS
  Genotyping of the GNAS T393C polymorphism in 196 prostate cancer patients and 200 healthy controls was performed by DNA extraction followed by PCR and restriction analysis.
  
  
  RESULTS
  We observed no evidence of effects related to GNAS T393C genotype as demonstrated by a comparison of the genotype distribution in prostate cancer patients and healthy controls, the genotype distribution dependent on grade of the primary diagnosis or data on clinical follow-up.
  
  
  CONCLUSIONS
  In conclusion, this study did not demonstrate an association between the GNAS T393C genotype and prostate cancer though such a relationship has been described for other cancer entities.
authors.affiliation: Praxisklinik Urologie Rhein-Ruhr, Mülheim an der Ruhr, Germany. Andreas.Eisenhardt @ googlemail.com
authors.forename: A
authors.initials: A
authors.lastname: Eisenhardt
authors.forename: A
authors.initials: A
authors.lastname: Scherag
authors.forename: K H
authors.initials: KH
authors.lastname: Jöckel
authors.forename: H
authors.initials: H
authors.lastname: Reis
authors.forename: H
authors.initials: H
authors.lastname: Rübben
authors.forename: W
authors.initials: W
authors.lastname: Siffert
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D002864:Chromogranins; D019205:GTP-Binding Protein alpha Subunits, Gs / Q000235:genetics*; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005858:Germany; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011468:Prostatectomy; D011471:Prostatic Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000401:mortality / Q000473:pathology / Q000601:surgery; D018570:Risk Assessment; D012307:Risk Factors; D013997:Time Factors; D016896:Treatment Outcome
chemical_list: D002864:Chromogranins; C406732:GNAS protein, human; D019205:GTP-Binding Protein alpha Subunits, Gs