title: Protein expression profile of human renal mesangial cells under high glucose.
abstract: BACKGROUND
  To understand the spectrum of proteins affected by diabetic nephropathy and to characterize the molecular functions and biological processes they control, the protein expression profile of human renal mesangial cells (HMCs) under high glucose was analyzed.
  
  
  METHODS
  HMCs were divided into a high glucose-cultured group (30 mmol/l) and a normal glucose-cultured group (5 mmol/l). The total proteins of the two groups were separated and analyzed by two-dimensional difference gel electrophoresis (DIGE). Spots that were differentially expressed were picked and digested with trypsin and subjected to MALDI-TOF MS for protein identification.
  
  
  RESULTS
  147 protein spots whose expression levels were significantly increased or decreased more than 1.5-fold in HMCs under high glucose culture were identified. 32 proteins were identified by peptide mass fingerprinting. The protein spots of phosphatidylethanolamine-binding protein 1, granulysin, ATP synthase, H(+) transporter, mitochondrial F0 complex and subunit F2 were observed only in the high glucose group. The expression of 24 proteins was upregulated by high glucose, including eosinophil cationic protein and others. The expression of 5 proteins was downregulated by high glucose, including proteasome β6 subunit precursor, among others.
  
  
  CONCLUSIONS
  32 protein expressions of human glomerular mesangial cells were regulated by high glucose. In-depth analysis of these differentially expressed proteins' function and crosstalk is expected to provide an experimental basis for clarifying the pathogenesis of diabetic nephropathy.
authors.affiliation: Central Laboratory, The First Hospital, China Medical University, Shenyang, China.
authors.forename: QiuLing
authors.initials: Q
authors.lastname: Fan
authors.forename: ShuYan
authors.initials: S
authors.lastname: Du
authors.forename: Gang
authors.initials: G
authors.lastname: Yang
authors.forename: LiNing
authors.initials: L
authors.lastname: Wang
authors.forename: Yi
authors.initials: Y
authors.lastname: Jiang
mesh_terms: D002478:Cells, Cultured; D003928:Diabetic Nephropathies / Q000378:metabolism; D015536:Down-Regulation; D005947:Glucose / Q000378:metabolism; D006801:Humans; D013058:Mass Spectrometry; D050527:Mesangial Cells / Q000378:metabolism*; D010449:Peptide Mapping; D011506:Proteins / Q000378:metabolism*; D057825:Two-Dimensional Difference Gel Electrophoresis; D015854:Up-Regulation
chemical_list: D011506:Proteins; D005947:Glucose