title: [Simultaneous determination of six flavonoids in Apocynum venetum by HPLC].
abstract: OBJECTIVE
  To develop a high-performance liquid chromatography (HPLC) method for the simultaneous determination of rutin, hyperoside, isoquercetin, astragulin, quercetin, and kaempferol in Apocynum venetum and its extracts.
  
  
  METHODS
  The separation was carried out on a Shim pack ODS (4.6 mm x 250 mm, 5 microm) colum eluted with in mobile phases of water containing 0.2% phosphoric acid and acetonitrile containing 0.2% phosphoric acid in acetonitrile gradient mode. The column temperature was 40 degrees C, and the flow rate was 1.0 mL x min(-1). The detection wavelength was set at 360 nm.
  
  
  RESULTS
  The good seperation of six flavonoids was achieved within 40 min, with the relative standard deviations (RSD) of intra- and inter-day precision < or = 2.0%. Calibration curves of rutin, hyperoside, isoquercetin, astragulin, quercetin, and kaempferol showed good linear relationship (R2 > 0.999 7, n = 6). The average recoveries of the six flavonoids were within 97.30% - 105.8% (RSD 2.6%). Three batches of A. venetum and 2 batches of its extracts were determined.
  
  
  CONCLUSIONS
  The developed method is simple, accurate, and repeatable, and can be readily used as a powerful tool for the quality control of A. venetum and its extracts.
authors.affiliation: Key Anhui Provincial key Lab of Natural Medicine Active Research, College of Pharmacy, Anhui Medical University, Hefei 230032, China. qunlinehang@163.com
authors.forename: Qunlin
authors.initials: Q
authors.lastname: Zhang
authors.forename: Liang
authors.initials: L
authors.lastname: Wu
authors.forename: Anding
authors.initials: A
authors.lastname: Yan
authors.forename: Feiyan
authors.initials: F
authors.lastname: Hu
authors.forename: Ye
authors.initials: Y
authors.lastname: Yuan
authors.forename: Juncheng
authors.initials: J
authors.lastname: Wang
mesh_terms: D030010:Apocynum / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D005419:Flavonoids / Q000032:analysis*; D011786:Quality Control; D015203:Reproducibility of Results
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids