title: Effects of dietary supplementation with phytonutrients on vaccine-stimulated immunity against infection with Eimeria tenella.
abstract: Two phytonutrient mixtures, VAC (carvacrol, cinnamaldehyde, and Capsicum oleoresin), and MC (Capsicum oleoresin and turmeric oleoresin), were evaluated for their effects on chicken immune responses following immunization with an Eimeria profilin protein. Chickens were fed with a non-supplemented diet, or with VAC- or MC-supplemented diets, immunized with profilin, and orally challenged with virulent oocysts of Eimeria tenella. Immunity against infection was evaluated by body weight, fecal oocyst shedding, profilin antibody levels, lymphocyte recall responses, cytokine expression, and lymphocyte subpopulations. Following immunization and infection, chickens fed the VAC- or MC-supplemented diets showed increased body weights, greater profilin antibody levels, and/or greater lymphocyte proliferation compared with non-supplemented controls. Prior to Eimeria infection, immunized chickens on the MC-supplemented diet showed reduced IFN-γ and IL-6 levels, but increased expression of TNFSF15, compared with non-supplemented controls. Post-infection levels of IFN-γ and IL-6 were increased, while IL-17F transcripts were decreased, with MC-supplementation. For VAC-supplemented diets, decreased IL-17F and TNFSF15 levels were observed only in infected chickens. Finally, immunized chickens fed the MC-supplemented diet exhibited increased MHC class II(+), CD4(+), CD8(+), TCR1+, or TCR2(+) T cells compared with nonsupplemented controls. Animals on the VAC-containing diet only displayed an increase in K1(+) macrophages. In conclusion, dietary supplementation with VAC or MC alters immune parameters following recombinant protein vaccination against avian coccidiosis.
authors.affiliation: Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Agricultural Research Service-U.S. Department of Agriculture, Beltsville, MD 20705, USA.
authors.forename: Sung Hyen
authors.initials: SH
authors.lastname: Lee
authors.forename: Hyun S
authors.initials: HS
authors.lastname: Lillehoj
authors.forename: Seung I
authors.initials: SI
authors.lastname: Jang
authors.forename: Kyung Woo
authors.initials: KW
authors.lastname: Lee
authors.forename: David
authors.initials: D
authors.lastname: Bravo
authors.forename: Erik P
authors.initials: EP
authors.lastname: Lillehoj
mesh_terms: D000171:Acrolein / Q000031:analogs & derivatives / Q000494:pharmacology; D000821:Animal Feed; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D000913:Antibodies, Protozoan; D002212:Capsicum / Q000737:chemistry; D049109:Cell Proliferation; D002645:Chickens; D003048:Coccidiosis / Q000517:prevention & control / Q000662:veterinary*; D030024:Curcuma / Q000737:chemistry; D000081007:Cymenes; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D019587:Dietary Supplements; D016786:Eimeria tenella; D005243:Feces / Q000469:parasitology; D005786:Gene Expression Regulation; D008214:Lymphocytes / Q000187:drug effects / Q000502:physiology; D039821:Monoterpenes / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D011201:Poultry Diseases / Q000469:parasitology / Q000517:prevention & control*; D051304:Profilins / Q000097:blood / Q000378:metabolism; D016052:Protozoan Vaccines / Q000276:immunology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013154:Spleen / Q000166:cytology; D015430:Weight Gain / Q000187:drug effects
chemical_list: D000913:Antibodies, Protozoan; D000081007:Cymenes; D016207:Cytokines; D039821:Monoterpenes; D010936:Plant Extracts; D051304:Profilins; D016052:Protozoan Vaccines; D012333:RNA, Messenger; C018620:oleoresins; D000171:Acrolein; C073316:carvacrol; C012843:cinnamaldehyde