title: Amacrine-to-amacrine cell inhibition: Spatiotemporal properties of GABA and glycine pathways.
abstract: We measured the spatial and temporal properties of GABAergic and glycinergic inhibition to amacrine cells in the whole-mount rabbit retina. The amacrine cells were parsed into two morphological classes: narrow-field cells with processes spreading less than 200 μm and wide-field cells with processes extending more than 300 μm. The inhibition was also parsed into two types: sustained glycine and transient GABA. Narrow-field amacrine cells receive 1) very transient GABAergic inhibition with a fast onset latency of 140 ± 16 ms decaying to 30% of the peak level within 208 ± 27 ms elicited broadly over a lateral distance of up to 1500 μm and 2) sustained glycinergic inhibition with a medium onset latency of 286 ± 23 ms that was elicited over a spatial area often broader than the processes of the narrow-field amacrine cells. Wide-field amacrine cells received sustained glycinergic inhibition but no broad transient GABAergic inhibition. Surprisingly, neither of these amacrine cell classes received sustained local GABAergic inhibition, commonly found in an earlier study of ganglion cells.
authors.affiliation: Department of Molecular and Cell Biology, University of California, Berkeley, USA.
authors.forename: Xin
authors.initials: X
authors.lastname: Chen
authors.forename: Hain Ann
authors.initials: HA
authors.lastname: Hsueh
authors.forename: Frank S
authors.initials: FS
authors.lastname: Werblin
mesh_terms: D000222:Adaptation, Physiological; D025042:Amacrine Cells / Q000187:drug effects / Q000502:physiology*; D000613:Aminobutyrates / Q000494:pharmacology; D000818:Animals; D002450:Cell Communication / Q000187:drug effects / Q000502:physiology*; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology; D005998:Glycine / Q000378:metabolism* / Q000494:pharmacology; D066298:In Vitro Techniques; D008027:Light; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D008954:Models, Biological; D009433:Neural Inhibition / Q000187:drug effects / Q000502:physiology*; D018408:Patch-Clamp Techniques / Q000379:methods; D011817:Rabbits; D011930:Reaction Time / Q000502:physiology; D012160:Retina / Q000166:cytology; D015398:Signal Transduction / Q000502:physiology; D014794:Visual Fields / Q000187:drug effects; D005680:gamma-Aminobutyric Acid / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D000613:Aminobutyrates; D018690:Excitatory Amino Acid Agonists; D005680:gamma-Aminobutyric Acid; C012729:2-amino-4-phosphonobutyric acid; D005998:Glycine