title: [Influence of exogenous 5'-nucleotides on acute alcohol intoxication in rats].
abstract: OBJECTIVE
  To investigate effects of exogenous 5'-nucleotides on acute alcohol intoxication in SD rats.
  
  
  METHODS
  In our study, 24 male SD rats were randomly divided into 4 groups which included a control group treated with normal saline and three experimental groups treated with low, medium and high doses of exogenous 5'-nucleotides (0.2, 0.8, 3.2 g/kg body weight). All the rats were gavaged with 50% ethanol 30 minutes after treatment. Then rotarod test and open field test were taken to assess rats' neurobehavior changes; Tail blood samples were collected to test blood ethanol concentration; Then all the rats were anesthetized and killed to collect blood and liver samples. Contents of serum alanine amino transferase, aspartate amino transferase, triglyceride, total cholesterol, high density lipoprotein cholesterol, total protein and albumin were tested; Their serum superoxide dismutase activity, malondialdehyde content and liver alcohol dehydrogenase activity were measured.
  
  
  RESULTS
  Compared with the controls, high dose nucleotides treated rats had lower serum ethanol concentration [(0.56±0.18 g/L)vs.(1.11±0.44 g/L), P<0.05]. However, exogenous 5'-nucleotides had no impact on neurobehavior and serum biochemical indicators; No difference was found in liver alcohol dehydrogenase activity, serum superoxide dismutase activity and malondialdehyde content were also found no different between the groups.
  
  
  CONCLUSIONS
  Exogenous 5'-nucleotides have no protective properties for acute alcohol intoxication in rats.
authors.affiliation: Department of Nutrition and Food Hygiene, Peking University School of Public Health, Beijing 100191, China.
authors.forename: Qing-hao
authors.initials: QH
authors.lastname: Jiang
authors.forename: Xia-xia
authors.initials: XX
authors.lastname: Cai
authors.forename: Lu-lu
authors.initials: LL
authors.lastname: Jing
authors.forename: Bing
authors.initials: B
authors.lastname: Lin
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.forename: Jun-bo
authors.initials: JB
authors.lastname: Wang
mesh_terms: D000208:Acute Disease; D000435:Alcoholic Intoxication / Q000188:drug therapy*; D000818:Animals; D008297:Male; D009711:Nucleotides / Q000627:therapeutic use*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D009711:Nucleotides