title: Determination of the kinetic on- and off-rate of single virus-cell interactions.
abstract: Human rhinoviruses are the causative agents of the common cold. The serotypes belonging to the minor receptor group attach to members of the low-density lipoprotein receptor family and enter the host cell via receptor-mediated endocytosis. Receptor binding, the very first step in infection, was characterized by force spectroscopy measurements at the single molecule level. We demonstrate how kinetic on- and off-rate constants can be derived from such experiments carried out with the atomic force microscope.
authors.affiliation: Institute for Biophysics, University of Linz, Linz, Austria.
authors.forename: Christian
authors.initials: C
authors.lastname: Rankl
authors.forename: Linda
authors.initials: L
authors.lastname: Wildling
authors.forename: Isabel
authors.initials: I
authors.lastname: Neundlinger
authors.forename: Ferry
authors.initials: F
authors.lastname: Kienberger
authors.forename: Hermann
authors.initials: H
authors.lastname: Gruber
authors.forename: Dieter
authors.initials: D
authors.lastname: Blaas
authors.forename: Peter
authors.initials: P
authors.lastname: Hinterdorfer
mesh_terms: D000818:Animals; D005347:Fibroblasts / Q000378:metabolism* / Q000821:virology*; D006801:Humans; D007700:Kinetics; D051379:Mice; D018625:Microscopy, Atomic Force; D011973:Receptors, LDL / Q000378:metabolism; D011991:Receptors, Virus / Q000378:metabolism*; D012229:Rhinovirus / Q000378:metabolism*
chemical_list: D011973:Receptors, LDL; D011991:Receptors, Virus