title: Longitudinal assessment of retinal structure and function reveals a rod-cone degeneration in a guinea pig model initially presented as night blind.
abstract: We have previously reported a naturally occurring retinopathy in a population of guinea pigs, where the affected animals presented a defect of the rod-mediated vision. The purpose of this study was to investigate if the mutants were affected with a stationary or degenerative retinopathy and to identify the cellular origin of this unique disorder. Electroretinogram (ERG) [postnatal day 1 (P1) to P450], light (LM) and electron microscopy (EM) [P5, P150, P450], and immunohistochemistry [P30, P150, P450] were evaluated from normal and mutant animals. Irrespective of age, the scotopic ERGs of mutants could only be evoked by bright flashes, and the resulting ERGs were of photopic waveform. Interestingly, the amplitude of the cone and the rod/cone a-waves was always of smaller amplitude in mutants, but this difference tended to decrease with age. In contrast, the b-waves were of larger amplitude than normal in photopic ERGs obtained prior to age 25 (days) and prior to age 10 for rod/cone ERGs. LM revealed, in mutants, an absence of the outer segment layer (OSL) with a reduction in the outer nuclear layer (ONL) thickness. EM disclosed the presence of cone outer segment (OS) while no rod OS could be evidenced. Immunohistochemistry revealed the presence of rhodopsin, both cone opsins as well as normal synaptophysin immunoreactivity. Finally, neither the retinal structure nor the function in the mutants achieved normal development. Results suggest that mutant animals are suffering from a degenerative retinal disorder that affects the structure and function of rods and cones.
authors.affiliation: Department of Ophthalmology (D-164), Montreal Children's Hospital-Research Institute, McGill University, QC, Canada. julie.racine@mail.mcgill.ca
authors.forename: Julie
authors.initials: J
authors.lastname: Racine
authors.forename: Sandrine
authors.initials: S
authors.lastname: Joly
authors.forename: Pierre
authors.initials: P
authors.lastname: Lachapelle
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015415:Biomarkers / Q000378:metabolism; D004195:Disease Models, Animal; D004596:Electroretinography; D015785:Eye Diseases, Hereditary; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D005500:Follow-Up Studies; D040181:Genetic Diseases, X-Linked; D006168:Guinea Pigs; D008297:Male; D008854:Microscopy, Electron; D009216:Myopia / Q000175:diagnosis / Q000235:genetics / Q000503:physiopathology*; D009755:Night Blindness / Q000175:diagnosis / Q000235:genetics / Q000503:physiopathology*; D010775:Photic Stimulation; D020419:Photoreceptor Cells, Vertebrate / Q000502:physiology* / Q000648:ultrastructure*; D012162:Retinal Degeneration / Q000175:diagnosis / Q000235:genetics / Q000503:physiopathology*
chemical_list: D015415:Biomarkers