title: Spectroscopic studies on interaction and sonodynamic damage of metallochlorophyllin (Chl-M (M=Fe, Zn and Cu)) to protein under ultrasonic irradiation.
abstract: In this paper, the chlorophyll derivatives, metallochlorophyllin (Chl-M) (M=Fe, Zn and Cu) including chlorophyllin iron (Chl-Fe), chlorophyllin zinc (Chl-Zn) and chlorophyllin copper (Chl-Cu), were adopted as sonosensitizers to combine with ultrasonic irradiation, and the sonodynamic damage of bovine serum albumin (BSA) was investigated. At first, the interaction of Chl-M with BSA was studied by fluorescence spectroscopy. The results show that the quenching mechanism belongs to a static process and among them the affinity of Chl-Fe to BSA is the most obvious. Then, some influence factors on the sonodynamic damage of BSA molecules in the presence of Chl-M under ultrasonic irradiation were also studied. Synchronous fluorescence spectra show that the binding and damage sites of Chl-M to BSA molecule are mainly on the tryptophan (Trp) residues. The generation of ROS in Chl-M sonodynamic process is estimated by the method of Oxidation-Extraction Spectrometry (OEP). This paper may offer some valuable references for the study of the sonodynamic activity of Chl-M and the effect of the central metals. Synchronously, it contributes to the application of Chl-M in SDT for tumor treatment.
authors.affiliation: College of Chemistry, Liaoning University, Shenyang 110036, PR China.
authors.forename: Jingqun
authors.initials: J
authors.lastname: Gao
authors.forename: Zhiqiu
authors.initials: Z
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Xudong
authors.initials: X
authors.lastname: Jin
authors.forename: Yuwei
authors.initials: Y
authors.lastname: Guo
authors.forename: Kai
authors.initials: K
authors.lastname: Li
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.forename: Pingli
authors.initials: P
authors.lastname: Kang
mesh_terms: D000818:Animals; D016587:Antimutagenic Agents / Q000378:metabolism / Q000528:radiation effects; D002417:Cattle; D002735:Chlorophyllides / Q000378:metabolism* / Q000528:radiation effects*; D006801:Humans; D009942:Organometallic Compounds / Q000378:metabolism / Q000528:radiation effects; D011485:Protein Binding / Q000528:radiation effects*; D011837:Radiation-Protective Agents / Q000378:metabolism / Q000528:radiation effects; D012710:Serum Albumin, Bovine / Q000378:metabolism* / Q000528:radiation effects*; D013050:Spectrometry, Fluorescence; D014465:Ultrasonics
chemical_list: D016587:Antimutagenic Agents; D002735:Chlorophyllides; D009942:Organometallic Compounds; D011837:Radiation-Protective Agents; D012710:Serum Albumin, Bovine; C007020:chlorophyllin