title: Antioxidant, antinociceptive, and anti-inflammatory properties of the ethanolic extract of Combretum duarteanum in rodents.
abstract: The antioxidant, antinociceptive, and anti-inflammatory activities of the ethanolic extract from leaves of Combretum duarteanum (EEC) were assessed in rodents through in vitro tests. The antioxidant activity was investigated by using thiobarbituric acid reactive species (TBARS), hydroxyl radical-scavenging, and scavenging activity of nitric oxide assays. The antinociceptive activity was investigated by using acetic acid-induced writhing, formalin, and hot-plate tests in mice. The anti-inflammatory activity was assessed in rats by using the carrageenan-induced hind-paw edema test and arachidonic acid-induced paw edema test. EEC possesses a strong antioxidant potential according to the TBARS, nitric oxide, and hydroxyl radical-scavenging assays; it also presented scavenger activity in all in vitro tests. After intraperitoneal injection, EEC (100, 200, and 400 mg/kg) significantly reduced the number of writhes (38.1%, 90.6%, and 97.8%, respectively) in a writhing test and the number of paw licks during phase 1 (30.5% and 69.5%, higher doses) and phase 2 (38.1%, 90.6%, and 97.8%, all doses) of a formalin test when compared with the control group. Naloxone (1.5 mg/kg, intraperitoneally) antagonized the antinociceptive action of EEC (400 mg/kg), and this finding suggests participation of the opioid system. Administration of 200 and 400 mg/kg (intraperitoneally) of EEC exhibited an anti-inflammatory activity in the carrageenin test, which was based on interference with prostaglandin synthesis. This finding was confirmed by the arachidonic acid test. Together, these results indicate that properties of EEC might be further explored in the search for newer tools to treat painful inflammatory conditions, including those related to pro-oxidant states.
authors.affiliation: Department of Physiology, Universidade Federal de Sergipe, São Cristóvão, Sergipe, Brazil.
authors.forename: Marcos G S
authors.initials: MG
authors.lastname: Gouveia
authors.forename: Maria A
authors.initials: MA
authors.lastname: Xavier
authors.forename: André S
authors.initials: AS
authors.lastname: Barreto
authors.forename: Daniel P
authors.initials: DP
authors.lastname: Gelain
authors.forename: João P A
authors.initials: JP
authors.lastname: Santos
authors.forename: Adriano A S
authors.initials: AA
authors.lastname: Araújo
authors.forename: Francilene A
authors.initials: FA
authors.lastname: Silva
authors.forename: Jullyana S
authors.initials: JS
authors.lastname: Quintans
authors.forename: Maria F
authors.initials: MF
authors.lastname: Agra
authors.forename: Analúcia G S
authors.initials: AG
authors.lastname: Cabral
authors.forename: Josean F
authors.initials: JF
authors.lastname: Tavares
authors.forename: Marcelo S
authors.initials: MS
authors.lastname: Silva
authors.forename: Lucindo J
authors.initials: LJ
authors.lastname: Quintans-Júnior
mesh_terms: D000700:Analgesics / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D016718:Arachidonic Acid / Q000633:toxicity; D002351:Carrageenan / Q000633:toxicity; D029765:Combretum / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy; D000431:Ethanol; D017665:Hydroxyl Radical / Q000032:analysis; D008297:Male; D051379:Mice; D009270:Naloxone / Q000378:metabolism; D009569:Nitric Oxide / Q000032:analysis; D010146:Pain / Q000188:drug therapy; D010147:Pain Measurement; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D012377:Rodentia; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
chemical_list: D000700:Analgesics; D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances; D016718:Arachidonic Acid; D009569:Nitric Oxide; D017665:Hydroxyl Radical; D009270:Naloxone; D000431:Ethanol; D002351:Carrageenan