title: Sequence elements outside the catalytic core of natural hairpin ribozymes modulate the reactions differentially.
abstract: Abstract Hairpin ribozymes occur naturally only in the satellite RNAs of tobacco ringspot virus (TRsV), chicory yellow mottle virus (CYMoV) and arabis mosaic virus (ArMV). The catalytic centre of the predominantly studied sTRsV hairpin ribozyme, and of sArMV is organised around a four-way helical junction. We show here that sCYMoV features a five-way helical junction instead. Mutational analysis indicates that the fifth stem does not influence kinetic parameters of the sCYMoV hairpin ribozyme in vitro reactions, and therefore seems an appendix to that junction in the other ribozymes. We report further that all three ribozymes feature a three-way helical junction outside the catalytic core in stem A, with Watson-Crick complementarity to loop nucleotides in stem B. Kinetic analyses of cleavage and ligation reactions of several variants of the sTRsV and sCYMoV hairpin ribozymes in vitro show that the presence of this junction interferes with their reactions, particularly the ligation. We provide evidence that this is not due to a presumed interaction of the afore-mentioned elements in stems A and B. The evolutionary survival of this cis-inhibiting element seems rather to be caused by the coincidence of its position with that of the hammerhead ribozyme in the other RNA polarity.
authors.affiliation: Heisenberg Research Group Ribogenetics, Technical University of Darmstadt, Germany.
authors.forename: Preeti
authors.initials: P
authors.lastname: Bajaj
authors.forename: Gerhard
authors.initials: G
authors.lastname: Steger
authors.forename: Christian
authors.initials: C
authors.lastname: Hammann
mesh_terms: D001483:Base Sequence; D020134:Catalytic Domain; D014644:Genetic Variation; D008958:Models, Molecular; D008969:Molecular Sequence Data; D015394:Molecular Structure; D012328:RNA Viruses / Q000201:enzymology; D016337:RNA, Catalytic / Q000737:chemistry / Q000235:genetics*; D019244:RNA, Satellite / Q000235:genetics*
chemical_list: D016337:RNA, Catalytic; D019244:RNA, Satellite; C418481:hairpin ribozyme