title: Surface modification of Ti-6Al-4 V alloy for biomineralization and specific biological response: part II, alkaline phosphatase grafting.
abstract: Titanium and its alloys are the most widespread materials for the realization of orthopaedic and dental implants due to their good mechanical properties and biocompatibility. Surface functionalization of biomaterials aimed to improve and quicken implant integration and tissue regeneration is an active research field. The opportunity to confer biological activity (ability to directly stimulate cells with proper biological signals) to the Ti6Al4 V alloy, previously modified to be bioactive from the inorganic point of view (apatite precipitation), was explored in this research work. The alkaline phosphatase (ALP) enzyme was grafted to metal surface via tresyl chloride activation, maintaining its activity. A synergistic effect between biological functionalization and inorganic bioactivity was observed.
authors.affiliation: DISMIC Department, Politecnico di Torino, C.so Duca degli Abruzzi, 24, 10129, Turin, Italy. sara.ferraris@polito.it
authors.forename: S
authors.initials: S
authors.lastname: Ferraris
authors.forename: S
authors.initials: S
authors.lastname: Spriano
authors.forename: C L
authors.initials: CL
authors.lastname: Bianchi
authors.forename: C
authors.initials: C
authors.lastname: Cassinelli
authors.forename: E
authors.initials: E
authors.lastname: Vernè
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism*; D000497:Alloys; D008903:Minerals / Q000378:metabolism*; D019736:Prostheses and Implants; D013499:Surface Properties; D014025:Titanium
chemical_list: D000497:Alloys; D008903:Minerals; C031462:titanium alloy (TiAl6V4); D014025:Titanium; D000469:Alkaline Phosphatase