title: Antioxidative and antigenotoxic effect of vitamin E against patulin cytotoxicity and genotoxicity in HepG2 cells.
abstract: Patulin (PAT) is a mycotoxin produced in fruits, mainly in apples, by certain species of Penicillium, Aspergillus, and Byssochlamys. It has been shown that PAT is cytotoxic, genotoxic, and mutagenic in different cell types. Several studies incriminate the oxidative stress as a mechanism of PAT-mediated toxicity. In this context, our aim was to investigate the protective role of Vitamin E (Vit E), an antioxidant agent, against PAT induced cytotoxicity and genotoxicity in cultured HepG2 cells. The obtained results showed that addition of Vit E in cells treated with PAT significantly reduce cell mortality induced by this toxin. In the same conditions, Vit E decreased the intracellular level of ROS, reduced PAT induced p53 expression, and reversed PAT induced DNA damage. In addition, Vit E prevented significantly the percentage of chromosome aberrations induced by PAT in HepG2 cells in a concentration dependant manner. These results suggest that Vit E, an exogenous antioxidant agent, plays an important role in defense against PAT-induced cytotoxicity and genotoxicity, which confirms the involvement of oxidative stress in the induction of DNA damage by PAT in HepG2 cells.
authors.affiliation: Laboratory for Research on Biologically Compatible Compounds-LRSBC, Monastir University, Rue Avicenne, Monastir 5019, Tunisia.
authors.forename: Imen
authors.initials: I
authors.lastname: Ayed-Boussema
authors.forename: Haila
authors.initials: H
authors.lastname: Abassi
authors.forename: Chayma
authors.initials: C
authors.lastname: Bouaziz
authors.forename: Wiem Ben
authors.initials: WB
authors.lastname: Hlima
authors.forename: Yosra
authors.initials: Y
authors.lastname: Ayed
authors.forename: Hassen
authors.initials: H
authors.lastname: Bacha
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D002273:Carcinogens / Q000633:toxicity*; D002470:Cell Survival / Q000187:drug effects; D002869:Chromosome Aberrations / Q000139:chemically induced; D004249:DNA Damage / Q000187:drug effects; D056945:Hep G2 Cells; D006801:Humans; D018384:Oxidative Stress / Q000187:drug effects; D010365:Patulin / Q000633:toxicity*; D014810:Vitamin E / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D002273:Carcinogens; D014810:Vitamin E; D010365:Patulin