title: Anti-hyaluronidase and anti-elastase activity screening of tannin-rich plant materials used in traditional Polish medicine for external treatment of diseases with inflammatory background.
abstract: BACKGROUND
  The aim of the study was to examine extracts from twelve tannin-rich plant materials used in traditional Polish medicine for external treatment of skin and mucosa diseases considering their ability to inhibit hyaluronidase activity and elastase release from stimulated neutrophils in vitro.
  
  
  METHODS
  In vitro anti-hyaluronidase and anti-elastase assays together with phytochemical qualitative and quantitative screening were performed.
  
  
  RESULTS
  The strongest inhibition of hyaluronidase was observed for extract from Lythri herba, with IC(50) value 8.1 ± 0.8 μg/mL. The most active extract towards elastase release was from Hippocastani cortex which at concentration 10 μg/mL showed 62.0 ± 6.9% inhibition.
  
  
  CONCLUSIONS
  Anti-hyaluronidase and anti-elastase activity of chosen tannin-rich plant materials can support their traditional use in folk medicine. Strong inhibition of both enzymes by extract from Lythri herba makes this pharmacopeial plant material an interesting topic for further biological and phytochemical examination.
authors.affiliation: Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Warsaw, Faculty of Pharmacy ul. Banacha 1, 02-097 Warsaw, Poland. jakub.piwowarski@wum.edu.pl
authors.forename: Jakub P
authors.initials: JP
authors.lastname: Piwowarski
authors.forename: Anna K
authors.initials: AK
authors.lastname: Kiss
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Kozłowska-Wojciechowska
mesh_terms: D000328:Adult; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001681:Biological Assay; D002855:Chromatography, Thin Layer; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D006821:Hyaluronoglucosaminidase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D019272:Leukocyte Elastase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D008519:Medicine, Traditional; D028321:Plant Preparations / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal; D011044:Poland; D013634:Tannins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D055815:Young Adult
chemical_list: D000893:Anti-Inflammatory Agents; D004791:Enzyme Inhibitors; D028321:Plant Preparations; D013634:Tannins; D006821:Hyaluronoglucosaminidase; D019272:Leukocyte Elastase