title: Ratiometric analysis of zidovudine (ZDV) incorporation by reverse transcriptases or polymerases via bio-orthogonal click chemistry.
abstract: A new fluorescence-based detection method was developed to visualize zidovudine-incorporated DNA using click chemistry. This bio-orthogonal detection method was used to quantify the relative susceptibility of various DNA-synthesizing enzymes toward ZDV incorporation on the basis of ratiometric analysis of band shifts in the gel and the visual observation of cellular DNA.
authors.affiliation: Department of Chemistry, Seoul National University, Seoul 151-747, Korea.
authors.forename: Minseob
authors.initials: M
authors.lastname: Koh
authors.forename: Jongmin
authors.initials: J
authors.lastname: Park
authors.forename: Heeseon
authors.initials: H
authors.lastname: An
authors.forename: Seung Bum
authors.initials: SB
authors.lastname: Park
mesh_terms: D000818:Animals; D019380:Anti-HIV Agents / Q000494:pharmacology*; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000378:metabolism*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000737:chemistry; D015658:HIV Infections / Q000188:drug therapy; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism*; D018894:Reverse Transcriptase Inhibitors / Q000494:pharmacology*; D015215:Zidovudine / Q000494:pharmacology*
chemical_list: D019380:Anti-HIV Agents; D005456:Fluorescent Dyes; D018894:Reverse Transcriptase Inhibitors; D015215:Zidovudine; D004247:DNA; D012194:RNA-Directed DNA Polymerase; D004259:DNA-Directed DNA Polymerase