title: Hypericum brasiliense plant extract neutralizes some biological effects of Bothrops jararaca snake venom.
abstract: Alternative treatments for snake bite are currently being extensively studied, and plant metabolites are considered good candidates for such purpose. Here, the ability of a crude ethanolic extract of Hypericum brasiliense plant in neutralizing Bothrops jararaca snake venom was investigated by in vitro (coagulation, hemolysis or proteolysis) and in vivo (hemorrhage, lethality and edema) biological assays. We describe for the first time the ability of H. brasiliense extracts to inhibit some pharmacological effects of a Brazilian snake venom. Inhibitory assays were performed by incubating B. jararaca venom with H. brasiliense extracts for 30min at room temperature before the assays were performed. The results showed that H. brasiliense extracts impaired lethality, edema, hemorrhage, hemolysis, proteolysis as well as fibrinogen or plasma clotting induced by B. jararaca venom. This indicates that H. brasiliense extracts can provide promising agents to treat B. jararaca envenomation.
authors.forename: Mariane
authors.initials: M
authors.lastname: Assafim
authors.forename: Eduardo Coriolano
authors.initials: EC
authors.lastname: de Coriolano
authors.forename: Sérgio Eufrázio
authors.initials: SE
authors.lastname: Benedito
authors.forename: Caio Pinho
authors.initials: CP
authors.lastname: Fernandes
authors.forename: Jonathas Felipe Revoredo
authors.initials: JF
authors.lastname: Lobo
authors.forename: Eladio Florez
authors.initials: EF
authors.lastname: Sanchez
authors.forename: Leandro Machado
authors.initials: LM
authors.lastname: Rocha
authors.forename: André Lopes
authors.initials: AL
authors.lastname: Fuly