title: Characterization of an intergenic polymorphic site (pp-hC1A_5) in Wolbachia pipientis (wPip).
abstract: Wolbachia pipientis (wPip) is an intracellular bacterium causing cytoplasmic incompatibility in arthropods, including mosquitoes of the Culex pipiens complex. Here, we present a method useful for genotyping within the wPip group. Primers were designed using a Tandem Repeat Finder program to amplify an intergenic, polymorphic site (pp-hC1A_5) of wPip. The polymorphic site is located between genes that code for polynucleotide phosphorylase and a hypothetical protein (C1A_5). Comparison of these wPip genomic regions from C. pipiens mosquitoes sampled in different geographic regions revealed deletions of fragments that proved useful in phylogenetic analysis.
authors.affiliation: Department of Parasitology and Infectious Diseases, Veterinary School, Aristotle University of Thessaloniki, Thessaloniki 54124, Greece. petridi@vet.auth.gr
authors.forename: Michael
authors.initials: M
authors.lastname: Petridis
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Chatzidimitriou
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D003465:Culex / Q000382:microbiology; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D021901:DNA, Intergenic; D005838:Genotype; D008969:Molecular Sequence Data; D058974:Phylogeography; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D020577:Wolbachia / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D021901:DNA, Intergenic