title: Production of γ-linolenic acid using a novel heterologous expression system in the oleaginous yeast Lipomyces kononenkoae.
abstract: A novel expression system was established in the oleaginous yeast, Lipomyces kononenkoae. The expression vector pLK-rhPHG of L. kononenkoae was constructed and using the hygromycin phosphotransferase gene and green fluorescent protein gene as reporter genes. A delta 6-fatty acid desaturase gene (D6DM) from Cunninghamella echinulata MIAN6 was then expressed in this strain. The recombinant strain accumulated about 1.2% γ-linolenic acid in the total fatty acids.
authors.affiliation: Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, 430062 Wuhan, People's Republic of China.
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.forename: Xia
authors.initials: X
authors.lastname: Wan
authors.forename: Yinbo
authors.initials: Y
authors.lastname: Zhang
authors.forename: Mulan
authors.initials: M
authors.lastname: Jiang
mesh_terms: D003001:Cloning, Molecular / Q000379:methods*; D020093:Cunninghamella / Q000201:enzymology / Q000235:genetics; D005231:Fatty Acids, Unsaturated / Q000032:analysis / Q000378:metabolism; D005822:Genetic Vectors; D050602:Linoleoyl-CoA Desaturase / Q000235:genetics / Q000378:metabolism*; D055160:Lipomyces / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017965:gamma-Linolenic Acid / Q000032:analysis / Q000096:biosynthesis*
chemical_list: D005231:Fatty Acids, Unsaturated; D011994:Recombinant Proteins; D017965:gamma-Linolenic Acid; D050602:Linoleoyl-CoA Desaturase