title: RAPD fingerprint to appraise the genetic fidelity of in vitro propagated Araucaria excelsa R. Br. var. glauca plantlets.
abstract: Randomly amplified polymorphic DNA (RAPD) was used as a tool to assess the genetic fidelity of in vitro propagated Araucaria excelsa R. Br. var. glauca with explants taken from orthotropic stem along with their related mother plants after treatment with kinetin, 2iP, BA (0.02-0.26 mg/l) and TDZ (0.001-1 mg/l) to produce axillary shoots. TDZ and kinetin induced more shoot and higher length per explant. Results showed a total of 1,676 fragments were generated with 12 RAPD primers in micropropagated plants and their donor mother plants. The number of loci ranged from 6 in OPB 12-18 in OPY 07 with a size ranging from 250 bp in OPH 19-3500 bp in OPH 11. Cluster analysis of RAPD data using UPGMA (unweighted pair group method with arithmetic average) revealed more than 92% genetic similarities between tissue cultured plants and their corresponding mother plant measured by the Jaccard's similarity coefficient. Similarity matrix and PCoA (two dimensional principal coordinate analysis) resulted in the same affinity. Primers had shown 36% polymorphism. However, careful monitoring of tissue culture derived plants might be needed to determine that rooted shoots are adventitious in origin.
authors.affiliation: Department of Horticultural Science, College of Agriculture, Shiraz University, Shiraz, Iran.
authors.forename: Mostafa Khoshhal
authors.initials: MK
authors.lastname: Sarmast
authors.forename: Hassan
authors.initials: H
authors.lastname: Salehi
authors.forename: Amin
authors.initials: A
authors.lastname: Ramezani
authors.forename: Ali Asghar
authors.initials: AA
authors.lastname: Abolimoghadam
authors.forename: Ali
authors.initials: A
authors.lastname: Niazi
authors.forename: Morteza
authors.initials: M
authors.lastname: Khosh-Khui
mesh_terms: D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D018744:DNA, Plant / Q000235:genetics* / Q000302:isolation & purification; D056426:Genetic Loci; D014644:Genetic Variation; D018517:Plant Roots / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D046509:Tissue Culture Techniques; D064028:Tracheophyta / Q000235:genetics* / Q000254:growth & development
chemical_list: D017931:DNA Primers; D018744:DNA, Plant