title: Complete sequence and gene organization of the mitochondrial genome of scaly-sided merganser (Mergus squamatus) and phylogeny of some Anatidae species.
abstract: The scaly-sided merganser (Mergus squamatus) is an endangered bird species on the IUCN Red List with the estimated global population of less than 2,500 individuals at present. In the present study, we studied the complete mitochondrial genome (mtDNA) and the phylogenetic of M. squamatus by PCR amplification and GenBank data. The genome was 16,595 bp in length and contained 37 genes (13 protein coding genes, two rRNAs, and 22 tRNAs) and a non-coding control region (D-loop). All protein-coding genes of M. squamatus mtDNA start with a typical ATG codon, except ND1, COI, and COII uses GTG as their initial codon. TAA, T- and TAG as the terminate codon occurred very commonly in the sequence. All tRNA genes can be folded into canonical cloverleaf secondary structure except for tRNA(Ser) (AGY) and tRNA(Leu) (CUN), which lose ''DHU'' arm. The genome sequences had been deposited in GenBank under accession number HQ833701. Based on the concatenated nucleotide sequences of mtDNA genes (Cyt b and D-loop), we reconstructed phylogenetic trees and discussed the phylogenetic relationships among ten Anatidae species. The results are different from the present classification, and we support Lophodytes cucullatus and Mergullus albellus to be members of the genus Mergus.
authors.affiliation: School of Resources and Environmental Engineering, Anhui University, Hefei, 230601 Anhui, People's Republic of China.
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
authors.forename: Li-zhi
authors.initials: LZ
authors.lastname: Zhou
authors.forename: Chang-ming
authors.initials: CM
authors.lastname: Gu
mesh_terms: D000818:Animals; D020029:Base Pairing; D001483:Base Sequence; D016000:Cluster Analysis; D003062:Codon / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D004372:Ducks / Q000145:classification / Q000235:genetics*; D056727:Endangered Species; D040481:Genome Components / Q000235:genetics; D054629:Genome, Mitochondrial / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010802:Phylogeny; D017422:Sequence Analysis, DNA
chemical_list: D003062:Codon; D017931:DNA Primers