title: Lack of effect of strain type on detection of toxigenic Clostridium difficile by glutamate dehydrogenase and polymerase chain reaction.
abstract: Glutamate dehydrogenase (GDH) is popular as a preliminary test for the detection of Clostridium difficile. Recent work has suggested that GDH sensitivity may vary according to ribotype and may be lower for ribotypes 002, 027, and 106 compared with polymerase chain reaction (PCR). We investigated this effect using a dilution series of 64 isolates tested by GDH and Cepheid GeneXpert PCR. PCR was significantly more sensitive than GDH overall; however, there was no difference in detection according to specific ribotype.
authors.affiliation: Directorate of Infection, Guy's & St. Thomas' NHS Foundation Trust, London SE1 7EH, UK. simon.goldenberg@kcl.ac.uk
authors.forename: Simon D
authors.initials: SD
authors.lastname: Goldenberg
authors.forename: Maria
authors.initials: M
authors.lastname: Gumban
authors.forename: Anthony
authors.initials: A
authors.lastname: Hall
authors.forename: Amita
authors.initials: A
authors.lastname: Patel
authors.forename: Gary L
authors.initials: GL
authors.lastname: French
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D016360:Clostridioides difficile / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification*; D003015:Clostridium Infections / Q000175:diagnosis* / Q000382:microbiology; D005969:Glutamate Dehydrogenase / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods*; D021521:Ribotyping; D012680:Sensitivity and Specificity
chemical_list: D005969:Glutamate Dehydrogenase