title: Trans-differentiation of a duodenal phenotype on duodenal transplantation of different normal tissues in F344 rats.
abstract: The mechanisms regulating stem cell differentiation and self-renewal are largely unknown. Our ultimate goal is to be able to regulate somatic stem cell differentiation and proliferation. In the present study the ability of trans-differentiation was studied when different normal tissue types were transplanted into the duodenum in rats. Pieces of ear (skin), bladder, trachea, diaphragm, pyloric gland, and forestomach from 8-week old GFP-F344 rats were transplanted into the duodenum of F344 rats. Goblet cells with alcian-blue PAS positive mucin and brash border with alkaline phosphatase (ALP) activity appeared in tissues implanted into the duodenum. In addition, GFP-positive duodenal mucosa was observed in all cases by immunohistochemical staining. Moreover, the GFP-positive cells were found to carry the GFP transgene by PCR analysis, indicating that the bladder, trachea, ear (skin), diaphragm, pyloric gland, and forestomach tissues showed a multipotential ability for differentiation. These results indicated that stem cells within tissues have a multipotential ability, trans-differentiating into different organs when transplanted into different environments.
authors.affiliation: Department of Experimental Oncology, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima 734-8553, Japan. tonko@hiroshima-u.ac.jp
authors.forename: Hiromitsu
authors.initials: H
authors.lastname: Watanabe
authors.forename: Yoko
authors.initials: Y
authors.lastname: Kominami
authors.forename: Rina
authors.initials: R
authors.lastname: Gon
authors.forename: Masaomi
authors.initials: M
authors.lastname: Hayashi
authors.forename: Masayo
authors.initials: M
authors.lastname: Nishiki
authors.forename: Atsushi
authors.initials: A
authors.lastname: Sasaki
authors.forename: Miho
authors.initials: M
authors.lastname: Shiraishi
authors.forename: Shinobu
authors.initials: S
authors.lastname: Ueda
authors.forename: Takahiro
authors.initials: T
authors.lastname: Ochiya
authors.forename: Kenji
authors.initials: K
authors.lastname: Kamiya
mesh_terms: D053687:Adult Stem Cells / Q000166:cytology; D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D002454:Cell Differentiation; D054338:Cell Transdifferentiation; D004386:Duodenum / Q000473:pathology* / Q000637:transplantation; D005260:Female; D049452:Green Fluorescent Proteins / Q000235:genetics; D008297:Male; D010641:Phenotype; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D051381:Rats; D011916:Rats, Inbred F344; D016042:Transplantation, Heterotopic
chemical_list: D018809:Proliferating Cell Nuclear Antigen; D049452:Green Fluorescent Proteins; D000469:Alkaline Phosphatase