title: Relationship between production of acute-phase proteins and strength of inflammatory stimulation in rats.
abstract: The relationship between intensity of inflammatory stimulation and production of α(2)-macroglobulin (α2M) and α(1)-acid glycoprotein (AAG) in rats was investigated. Sprague-Dawley rats were injected with turpentine oil at doses of 0.05, 0.2 or 0.4 mL/rat. Serum levels of α2M, interleukin (IL)-6 and cytokine-induced neutrophil chemoattractant-1 (CINC-1) were measured by enzyme-linked immunosorbent assay, and AAG was measured by single radial immunodiffusion. Peak serum levels of α2M and AAG in rats injected at 0.05 mL/rat were significantly lower than those at 0.2 or 0.4 mL/rat. However, no significant differences were observed for peak serum levels of these acute-phase proteins between 0.2 and 0.4 mL/rat. Furthermore, peak serum levels of IL-6 and CINC-1 in rats injected at 0.05 mL/rat were significantly lower than those at 0.2 or 0.4 mL/rat. Thus, the production of these acute-phase proteins has upper limits, even under increased strength of inflammatory stimulation in rats injected with turpentine oil.
authors.affiliation: Laboratory of Immunology, School of Life and Environmental Science, Azabu University, 1-17-71 Fuchinobe, Chuo-ku, Sagamihara, Kanagawa 252-5201, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Kuribayashi
authors.forename: Misaki
authors.initials: M
authors.lastname: Tomizawa
authors.forename: Tetsurou
authors.initials: T
authors.lastname: Seita
authors.forename: Kazutoshi
authors.initials: K
authors.lastname: Tagata
authors.forename: Shizuo
authors.initials: S
authors.lastname: Yamamoto
mesh_terms: D000210:Acute-Phase Reaction / Q000139:chemically induced*; D000818:Animals; D054360:Chemokine CXCL1 / Q000097:blood / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005779:Immunodiffusion; D015850:Interleukin-6 / Q000097:blood / Q000378:metabolism*; D009961:Orosomucoid / Q000032:analysis / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014425:Turpentine / Q000008:administration & dosage / Q000633:toxicity; D000511:alpha-Macroglobulins / Q000032:analysis / Q000096:biosynthesis*
chemical_list: D054360:Chemokine CXCL1; C513297:Cxcl1 protein, rat; D015850:Interleukin-6; C506108:Orm1 protein, rat; D009961:Orosomucoid; D000511:alpha-Macroglobulins; D014425:Turpentine