title: Pterostilbene suppresses benzo[a]pyrene-induced airway remodeling.
abstract: This study has two novel findings: it is not only the first to demonstrate inflammatory cytokines, which are produced by the bronchial epithelium after exposure to benzo[a]pyrene (BaP) and contribute to airway remodeling by increasing human bronchial smooth muscle cells (BSMC) proliferation and migration, but also the first to reveal that pterostilbene, a constituent of grapes and berries, reverses BaP-mediated airway remodeling. Human bronchial epithelial cell lines BEAS-2B and HBE135-E6E7 (HBE) were treated with BaP, and then the condition medium (CM) was harvested, which was then added to BSMC. Cultures of BSMC with BaP-BEAS-2B-CM and -HBE-CM increased BSMC proliferation and migration, which are major features in asthma remodeling. Exposure of BEAS-2B and HBE to BaP caused epithelial cells to produce inflammatory cytokines IL-8, which subsequently induced BSMC proliferation and migration. Moreover, pterostilbene is more potent than resveratrol in suppressing BaP-mediated airway remodeling. This study suggests that pterostilbene is capable of preventing BaP-associated asthma.
authors.affiliation: Institute of Clinical Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors.forename: Po-Lin
authors.initials: PL
authors.lastname: Kuo
authors.forename: Ya-Ling
authors.initials: YL
authors.lastname: Hsu
authors.forename: Ming-Ju
authors.initials: MJ
authors.lastname: Tsai
authors.forename: Ming-Shyan
authors.initials: MS
authors.lastname: Huang
mesh_terms: D056151:Airway Remodeling / Q000187:drug effects*; D001564:Benzo(a)pyrene / Q000494:pharmacology*; D001980:Bronchi / Q000166:cytology / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D015536:Down-Regulation; D006801:Humans; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000187:drug effects; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D013267:Stilbenes; C107773:pterostilbene; D001564:Benzo(a)pyrene