title: Jacaranda cuspidifolia Mart. (Bignoniaceae) as an antibacterial agent.
abstract: This study evaluated, in vitro, the antimicrobial activity of the hexane extract (JCHE), methanol extract (JCME), and chloroform fraction (JCCF) of bark from Jacaranda cuspidifolia Mart. (Family Bignoniaceae), a Brazilian medicinal plant, traditionally used as anti-syphilis and anti-gonorrhea treatment. The antimicrobial activity was evaluated using the disc diffusion method followed by the determination of minimum inhibitory concentration (MIC) values. JCHE was not active against the bacteria evaluated. JCME presented antibacterial activity against Streptococcus pyogenes, Staphylococcus aureus, and Neisseria gonorrhoeae with MIC values of 16.3 mg/mL, 9.1 mg/mL, and 25.2 mg/mL, respectively. JCCF was active against Staphylococcus epidermidis, S. aureus, Proteus mirabilis, Serratia marcescens, S. pyogenes, Enterobacter aerogenes, and N. gonorrhoeae with MIC values of 18.3 mg/mL, 9.3 mg/mL, 6.3 mg/mL, 6.1 mg/mL, 9.2 mg/mL, 6.2 mg/mL, and 25.2 mg/mL, respectively. Phytochemical analysis of JCME and JCCF gave positive results for saponins, coumarins, flavonoids, tannins, quinones, alkaloids, triterpenes, and steroids. Verbascoside was isolated and identified as a major peak in JCME and JCCF high-performance liquid chromatography fingerprints and might contribute to the observed antimicrobial activity.
authors.affiliation: Course of Pharmacy, Department of Biological Sciences and Health, Dom Bosco Catholic University, Campo Grande, Mato Grosso do Sul, Brazil.
authors.forename: Ana Lúcia A
authors.initials: AL
authors.lastname: Arruda
authors.forename: Carla J B
authors.initials: CJ
authors.lastname: Vieira
authors.forename: Daniella G
authors.initials: DG
authors.lastname: Sousa
authors.forename: Regilene F
authors.initials: RF
authors.lastname: Oliveira
authors.forename: Rachel O
authors.initials: RO
authors.lastname: Castilho
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D029402:Bignoniaceae / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D021902:Enterobacter aerogenes / Q000187:drug effects; D005960:Glucosides / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D009344:Neisseria gonorrhoeae / Q000187:drug effects; D010636:Phenols / Q000494:pharmacology; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry; D011513:Proteus mirabilis / Q000187:drug effects; D012706:Serratia marcescens / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects; D013212:Staphylococcus epidermidis / Q000187:drug effects; D013297:Streptococcus pyogenes / Q000187:drug effects; D059808:Polyphenols
chemical_list: D000900:Anti-Bacterial Agents; D005960:Glucosides; D010636:Phenols; D010936:Plant Extracts; C058956:acteoside; D059808:Polyphenols