title: Induced in vitro differentiation of neural-like cells from human exfoliated deciduous teeth-derived stem cells.
abstract: Stem cells from human exfoliated deciduous teeth (SHED) are highly proliferative, clonogenic and multipotent stem cells with a neural crest cell origin. Additionally, they can be collected with minimal invasiveness in comparison with other sources of mesenchymal stem cells (MSCs). Therefore, SHED could be a desirable option for potential therapeutic applications. In this study, SHEDs were established from enzyme-disaggregated deciduous dental pulp obtained from 6 to 9 year-old children. The cells had typical fibroblastoid morphology and expressed antigens characteristic of MSCs, STRO1, CD146, CD45, CD90, CD106 and CD166, but not the hematopoietic and endothelial markers, CD34 and CD31, as assessed by FACS analysis. Differentiation assessment revealed a strong osteogenic and adipogenic potential of SHEDs. In order to further evaluate the in vitro differentiation potential of SHED into neural cells, a simple short time growth factor-mediated induction was used. Immunofluorescence staining and flow cytometric analysis revealed that SHED rapidly expressed nestin and b-III tubulin, and later expressed intermediate neural markers. In addition, the intensity and percentages of nestin and b-III tubulin and mature neural markers (PSA-NCAM, NeuN, Tau, TH, or GFAP) increased significantly following treatment. Moreover, RT-PCR and Western blot analyses showed that the neural markers were strongly up-regulated after induction. In conclusion, these results provide evidence that SHED can differentiate into neural cells by the expression of a comprehensive set of genes and proteins that define neural-like cells in vitro. SHED cells might be considered as new candidates for the autologous transplantation of a wide variety of neurological diseases and neurotraumatic injuries.
authors.affiliation: Pediatric Department, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Nosrat
authors.initials: N
authors.lastname: Nourbakhsh
authors.forename: Mitra
authors.initials: M
authors.lastname: Soleimani
authors.forename: Zahra
authors.initials: Z
authors.lastname: Taghipour
authors.forename: Khadijeh
authors.initials: K
authors.lastname: Karbalaie
authors.forename: Seeid-Behrouz
authors.initials: SB
authors.lastname: Mousavi
authors.forename: Ardeshir
authors.initials: A
authors.lastname: Talebi
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Nadali
authors.forename: Somayeh
authors.initials: S
authors.lastname: Tanhaei
authors.forename: Gholam-Abbas
authors.initials: GA
authors.lastname: Kiyani
authors.forename: Marziyeh
authors.initials: M
authors.lastname: Nematollahi
authors.forename: Farzaneh
authors.initials: F
authors.lastname: Rabiei
authors.forename: Mohammad
authors.initials: M
authors.lastname: Mardani
authors.forename: Hamid
authors.initials: H
authors.lastname: Bahramiyan
authors.forename: Mahmood
authors.initials: M
authors.lastname: Torabinejad
authors.forename: Mohammad-Hossein
authors.initials: MH
authors.lastname: Nasr-Esfahani
authors.forename: Hossein
authors.initials: H
authors.lastname: Baharvand
mesh_terms: D015415:Biomarkers; D015153:Blotting, Western; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D002648:Child; D003782:Dental Pulp / Q000166:cytology* / Q000196:embryology; D006801:Humans; D007381:Intermediate Filament Proteins / Q000096:biosynthesis; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000096:biosynthesis; D064231:Nestin; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D014080:Tooth Exfoliation; D014094:Tooth, Deciduous / Q000166:cytology*; D014404:Tubulin / Q000096:biosynthesis
chemical_list: D015415:Biomarkers; D007381:Intermediate Filament Proteins; C577484:NES protein, human; D009419:Nerve Tissue Proteins; D064231:Nestin; D014404:Tubulin