title: Transformation of prednisolone to a 20β-hydroxy prednisolone compound by Streptomyces roseochromogenes TS79.
abstract: Prednisolone represents an important compound in pharmaceutical preparations. To obtain more bioactive prednisolone derivatives, the microbial transformation of prednisolone was carried out. The steroid products were assigned by an interpretation of their spectral data using mass spectrometry and proton nuclear magnetic resonance ((1)H NMR) analyses. The product was assigned the chemical structure of 11β, 17α, 20β, 21-tetrahydroxypregna-1,4-diene-3-one (named as 20β-hydroxy prednisolone). The conversion of prednisolone to 20β-hydroxy prednisolone by Streptomyces roseochromogenes TS79 was different from a previous study on the microbial transformation of steroid by this organism, which usually generates a 16α-hydroxy steroid product. The different reaction parameters for maximum conversion of prednisolone were optimized. The analysis revealed that the optimum values of the tested variables were 7.5 mg/ml prednisolone dissolved in DMSO and added to the 24-h pre-culture fermentation culture containing 0.05% MgSO(4) and incubated for 24 h. A conversion of 95.1% of prednisolone was observed, which has the potential to be used in industrial production.
authors.affiliation: School of Life Sciences, Tsinghua University, Beijing 100084, China.
authors.forename: Wenquan
authors.initials: W
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Cui
authors.forename: Mengyao
authors.initials: M
authors.lastname: Wu
authors.forename: Rongqing
authors.initials: R
authors.lastname: Zhang
authors.forename: Liping
authors.initials: L
authors.lastname: Xie
authors.forename: Hongzhong
authors.initials: H
authors.lastname: Wang
mesh_terms: D001711:Biotransformation; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D009682:Magnetic Resonance Spectroscopy; D011239:Prednisolone / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism*; D013302:Streptomyces / Q000378:metabolism*
chemical_list: D003470:Culture Media; D011239:Prednisolone