title: Lipotropes (methyl nutrients) inhibit growth of feline lymphoma in vitro.
abstract: Feline lymphoma is one of the most frequently diagnosed tumors in cats. Lipotropes are dietary methyl donors that may modulate DNA methylation status and the expression of genes involved in growth and apoptosis of feline lymphoma cells. The specific objective of the study was to determine if lipotropes affect the growth of feline lymphoma cells, which entailed examining a correlation between lymphoma cell proliferation and apoptosis. F1B and FeLV-3281 cells were cultured and treated with 20 times the level of lipotropes contained in the basal culture medium. Cell growth and death and caspase 3 and tumor protein p53 activity were measured. Lipotropes were found to significantly reduce cell growth; increased cell death and caspase 3 and p53 activity was seen in F1B cells after 72 h, but the effect was minimal on FeLV-3281. These results could be useful in the development of dietary strategies for treating and preventing feline lymphoma.
authors.affiliation: Department of Animal Sciences, North Dakota State University, Fargo, ND 58108, USA.
authors.forename: L
authors.initials: L
authors.lastname: Mabasa
authors.forename: K
authors.initials: K
authors.lastname: Cho
authors.forename: S
authors.initials: S
authors.lastname: Bae
authors.forename: D M
authors.initials: DM
authors.lastname: Walsh
authors.forename: P
authors.initials: P
authors.lastname: Asija
authors.forename: C S
authors.initials: CS
authors.lastname: Park
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D002371:Cat Diseases / Q000188:drug therapy*; D002415:Cats; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry / Q000662:veterinary; D066298:In Vitro Techniques; D050356:Lipid Metabolism / Q000187:drug effects*; D008223:Lymphoma / Q000737:chemistry / Q000188:drug therapy / Q000662:veterinary*; D008745:Methylation / Q000187:drug effects; D016159:Tumor Suppressor Protein p53 / Q000032:analysis
chemical_list: D000970:Antineoplastic Agents; D016159:Tumor Suppressor Protein p53; D053148:Caspase 3