title: Frequency of CXCR4-using viruses in primary HIV-1 infections using ultra-deep pyrosequencing.
abstract: We used ultra-deep pyrosequencing and the Toulouse Tropism Test phenotypic assay to determine the prevalence of CXCR4-using viruses in 21 patients with primary HIV-1 infections. We found X4-containing virus populations in 9% of patients by ultra-deep pyrosequencing using position-specific scoring matrices (PSSM(X4/R5)) or geno2pheno(5.75) and in 14% using the combined 11/25 and net charge rule. The phenotypic assay identified 9% of CXCR4-using viruses. This confirms that R5 viruses are predominant in primary HIV-1 infections.
authors.affiliation: INSERM U, Toulouse, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Raymond
authors.forename: Adrien
authors.initials: A
authors.lastname: Saliou
authors.forename: Florence
authors.initials: F
authors.lastname: Nicot
authors.forename: Pierre
authors.initials: P
authors.lastname: Delobel
authors.forename: Martine
authors.initials: M
authors.lastname: Dubois
authors.forename: Michelle
authors.initials: M
authors.lastname: Cazabat
authors.forename: Karine
authors.initials: K
authors.lastname: Sandres-Sauné
authors.forename: Bruno
authors.initials: B
authors.lastname: Marchou
authors.forename: Patrice
authors.initials: P
authors.lastname: Massip
authors.forename: Jacques
authors.initials: J
authors.lastname: Izopet
mesh_terms: D000328:Adult; D005602:France; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000235:genetics*; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D008875:Middle Aged; D012367:RNA, Viral; D019713:Receptors, CCR5 / Q000235:genetics*; D019718:Receptors, CXCR4 / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods; D014764:Viral Proteins / Q000235:genetics
chemical_list: D012367:RNA, Viral; D019713:Receptors, CCR5; D019718:Receptors, CXCR4; D014764:Viral Proteins