title: [Optimization of the hydrolysis process for C21 steroidal glycoside of bai shou wu by acetic acid with multi-target orthogonal design].
abstract: OBJECTIVE
  To study the optimizal hydrolysis process for C21 steroidal glycoside of Bai Shou Wu by acetic acid.
  
  
  METHODS
  The effects of acetic acid concentration, reaction temperature and reaction time had been investigated using orthogonal design and the contents of kidjoranin 3-O-beta-digitoxopyranoside, caudatin, kidjoranin 3-O-alpha-L-diginopyranosyl-(1 --> 4)-beta-cymaropyranoside and caudatin 3-O-beta-cymaropyranoside as response indexs were determined by the high performance liquid chromatography.
  
  
  RESULTS
  The factors influencing acetic extraction efficiency were as follows: A > B > C (A. reaction temperature; B. reaction time; C. acetic acid concentration). The optimal hydrolysis condition obtained was: refluxing for 6 hours with 5.0% dilute CH3COOH solution at 100 degrees C.
  
  
  CONCLUSIONS
  The content of antitumor active ingredients under the optimum hydrolysis condition is raised obviously and has a great part in studying this antitumor drug.
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhao
authors.forename: Mei
authors.initials: M
authors.lastname: Chen
authors.forename: Yunru
authors.initials: Y
authors.lastname: Peng
authors.forename: Youbin
authors.initials: Y
authors.lastname: Li
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D031105:Cynanchum / Q000737:chemistry / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000378:metabolism; D006027:Glycosides / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D006868:Hydrolysis / Q000187:drug effects*; D013696:Temperature; D013997:Time Factors
chemical_list: D000085:Acetates; D004365:Drugs, Chinese Herbal; D006027:Glycosides