title: Nanostructured functional co-polymers bioconjugate integrin inhibitors.
abstract: Synthesis and properties of bioconjugates based on functionalized polymeric nanoparticles (PNs) and monoclonal anti-Integrin αV CD51 (aI) antibody were investigated. Polymeric and co-polymeric colloidal nanoparticles with different functionalities, i.e., acid, amine, or thiol, namely poly(methylmethacrylate-co-acrylic acid) [P(MMA-co-AA)], poly(methylmethacrylate-co-dimethylpropargylamine) [P(MMA-co-DMPA)], poly(methylmethacrylate-co-allil mercaptane) [P(MMA-co-AM)], were obtained by tailoring emulsion synthesis and fully characterized by means of spectroscopic techniques and scanning electron microscopy (SEM). Bioconjugates (PN/aI) based on P(MMA) or P(MMA-co-AA) were obtained by loading the polymeric nanoparticles with the antibody anti-Integrin with a simple and straightforward immobilization strategy. Bioconjugates qualitative and quantitative loading analyses were carried out by means of polyacrylamide gel electrophoresis 1D-PAGE, MALDI-TOF, and LC/ESI-MS/MS investigations. The biological efficacy of bioconjugates was confirmed by the reduced migration potential of PN/aI-treated human kidney cells (HEK293). The easy immobilization procedure and high immobilization capacity of polymeric nanoparticles together with tuneable chemical functionalities and dimension of the polymeric nanoparticles open applicative perspectives for targeted delivery.
authors.affiliation: Department of Chemistry, University of Rome Sapienza, P.le A. Moro 5, I-00185 Rome, Italy.
authors.forename: Aldo
authors.initials: A
authors.lastname: Laganà
authors.forename: Iole
authors.initials: I
authors.lastname: Venditti
authors.forename: Ilaria
authors.initials: I
authors.lastname: Fratoddi
authors.forename: Anna Laura
authors.initials: AL
authors.lastname: Capriotti
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Caruso
authors.forename: Chiara
authors.initials: C
authors.lastname: Battocchio
authors.forename: Giovanni
authors.initials: G
authors.lastname: Polzonetti
authors.forename: Filippo
authors.initials: F
authors.lastname: Acconcia
authors.forename: Maria
authors.initials: M
authors.lastname: Marino
authors.forename: Maria Vittoria
authors.initials: MV
authors.lastname: Russo
mesh_terms: D000588:Amines / Q000737:chemistry; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D002465:Cell Movement / Q000187:drug effects*; D003102:Colloids / Q000737:chemistry; D004337:Drug Carriers / Q000138:chemical synthesis / Q000378:metabolism; D016503:Drug Delivery Systems / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D057809:HEK293 Cells; D006801:Humans; D018796:Immunoconjugates / Q000737:chemistry / Q000276:immunology / Q000494:pharmacology*; D039564:Integrin alphaV / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D020366:Methylmethacrylate / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D011108:Polymers / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013438:Sulfhydryl Compounds / Q000737:chemistry
chemical_list: D000588:Amines; D000911:Antibodies, Monoclonal; D003102:Colloids; D004337:Drug Carriers; D018796:Immunoconjugates; D039564:Integrin alphaV; D011108:Polymers; D013438:Sulfhydryl Compounds; D020366:Methylmethacrylate