title: Analysis of collagen expression during chondrogenic induction of human bone marrow mesenchymal stem cells.
abstract: Adult mesenchymal stem cells (MSCs) are currently being investigated as an alternative to chondrocytes for repairing cartilage defects. As several collagen types participate in the formation of cartilage-specific extracellular matrix, we have investigated their gene expression levels during MSC chondrogenic induction. Bone marrow MSCs were cultured in pellet in the presence of BMP-2 and TGF-β3 for 24 days. After addition of FGF-2, at the fourth passage during MSC expansion, there was an enhancing effect on specific cartilage gene expression when compared to that without FGF-2 at day 12 in pellet culture. A switch in expression from the pre-chondrogenic type IIA form to the cartilage-specific type IIB form of the collagen type II gene was observed at day 24. A short-term addition of FGF-2 followed by a treatment with BMP-2/TGF-β3 appears sufficient to accelerate chondrogenesis with a particular effect on the main cartilage collagens.
authors.affiliation: Institut de Biologie et Chimie des Protéines, Université Lyon 1, Univ Lyon, CNRS FRE 3310, IFR128 BioSciences Gerland-Lyon Sud, 7 Passage du Vercors, 69367 Lyon Cedex 7, France. emeline.groult@ibcp.fr
authors.forename: Emeline
authors.initials: E
authors.lastname: Perrier
authors.forename: Marie-Claire
authors.initials: MC
authors.lastname: Ronzière
authors.forename: Reine
authors.initials: R
authors.lastname: Bareille
authors.forename: Astrid
authors.initials: A
authors.lastname: Pinzano
authors.forename: Frédéric
authors.initials: F
authors.lastname: Mallein-Gerin
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Freyria
mesh_terms: D000328:Adult; D000368:Aged; D001854:Bone Marrow Cells / Q000187:drug effects / Q000378:metabolism*; D055396:Bone Morphogenetic Protein 2 / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D020219:Chondrogenesis / Q000187:drug effects / Q000502:physiology*; D003094:Collagen / Q000096:biosynthesis* / Q000235:genetics; D024043:Collagen Type II / Q000235:genetics / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D007150:Immunohistochemistry; D059630:Mesenchymal Stem Cells / Q000187:drug effects / Q000378:metabolism*; D008875:Middle Aged; D020033:Protein Isoforms; D053782:Transforming Growth Factor beta3 / Q000494:pharmacology
chemical_list: D055396:Bone Morphogenetic Protein 2; C499177:COL2A1 protein, human; D024043:Collagen Type II; D020033:Protein Isoforms; D053782:Transforming Growth Factor beta3; D016222:Fibroblast Growth Factor 2; D003094:Collagen