title: Bioanalytical approaches to analyzing peptides and proteins by LC--MS/MS.
abstract: Peptides and proteins have been utilized as therapeutic agents for over 40 years. Traditional approaches to quantify these molecules in biological matrices have utilized immunoassay approaches that can be time inefficient, lack assay specificity and have limited analytical ranges. The advances in sample preparation technologies, chromatographic systems and their chemistries, mass spectrometers and their software over the last decade have meant that LC-MS/MS approaches to peptide and protein quantification are feasible and can overcome the problems associated with quantification by immunoassay. In this article we present an overview of the challenges and approaches to overcome them when performing quantitative bioanalysis of peptides and proteins by LC-MS/MS.
authors.affiliation: Bioanalytical Services, Covance Laboratories, Harrogate, HG3 1PY, UK. matthew.ewles@ covance.com
authors.forename: Matthew
authors.initials: M
authors.lastname: Ewles
authors.forename: Lee
authors.initials: L
authors.lastname: Goodwin
mesh_terms: D000327:Adsorption; D002853:Chromatography, Liquid / Q000379:methods*; D004355:Drug Stability; D006801:Humans; D007118:Immunoassay; D010455:Peptides / Q000097:blood*; D004364:Pharmaceutical Preparations / Q000097:blood*; D011506:Proteins / Q000032:analysis*; D012015:Reference Standards; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014357:Trypsin / Q000378:metabolism
chemical_list: D010455:Peptides; D004364:Pharmaceutical Preparations; D011506:Proteins; D014357:Trypsin