title: Adenovirus-mediated transfer of tris-shRNAs induced apoptosis of nasopharyngeal carcinoma cell in vitro and in vivo.
abstract: RNA interference (RNAi) is an evolutionary conserved mechanism for specific gene silencing. There are currently numerous cancer therapy clinical trials based on RNAi technology. Using an adenoviral system as a delivery mediator of RNAi, we investigated the therapeutic effects of targeting three genes simultaneously in vitro and in vivo. In this study, we constructed an recombinant adenoviral shRNA expression system as Adv-pEGFP-shVEGF-shTERT-shBcl-xl for multi-genes silencing. Our results showed that the adenoviral vector can achieve above 90% of transfection efficiency and induced obvious apoptosis in CNE-2 cell both in vitro and in vivo compared with targeting the TERT alone or controlled group.
authors.affiliation: Department of Otolaryngology, Head & Neck Surgery, Renmin Hospital of Wuhan University, Wuhan, PR China.
authors.forename: Ji-Bo
authors.initials: JB
authors.lastname: Han
authors.forename: Ze-Zhang
authors.initials: ZZ
authors.lastname: Tao
authors.forename: Shi-Ming
authors.initials: SM
authors.lastname: Chen
authors.forename: Yong-Gang
authors.initials: YG
authors.lastname: Kong
authors.forename: Bo-Kui
authors.initials: BK
authors.lastname: Xiao
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D017209:Apoptosis; D015153:Blotting, Western; D002277:Carcinoma; D045744:Cell Line, Tumor; D005434:Flow Cytometry; D005822:Genetic Vectors; D006801:Humans; D007211:Indoles; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D000078183:Oxindoles; D016133:Polymerase Chain Reaction; D034622:RNA Interference; D034741:RNA, Small Interfering; D019098:Telomerase / Q000235:genetics; D014162:Transfection; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics; D023041:Xenograft Model Antitumor Assays; D051020:bcl-X Protein / Q000235:genetics
chemical_list: C494811:BCL2L1 protein, human; D007211:Indoles; D000078183:Oxindoles; D034741:RNA, Small Interfering; D042461:Vascular Endothelial Growth Factor A; D051020:bcl-X Protein; C016857:152ORB; C509186:TERT protein, human; D019098:Telomerase