title: Microchip flow cytometry using electrokinetic focusing.
abstract: Flow cytometry of fluorescently labeled and unlabeled latex particles is demonstrated on a microfabricated device. The latex particles were detected and counted using laser light scattering and fluorescence coincidence measurements. Sample confinement was accomplished using electrokinetic focusing at a cross intersection, and detection occurred 50 μm downstream from the intersection. Particles with diameters of 1 and 2 μm were analyzed and distinguished from each other based on their light scattering intensity and fluorescence. A maximum sample throughput of 34 particles/s was achieved. Sample mixtures with varying proportions of fluorescently labeled and unlabeled particles were also analyzed and found to be within experimental error of the expected ratios.
authors.affiliation: Oak Ridge National Laboratory, P.O. Box 2008, Oak Ridge, Tennessee 37831-6142.
authors.forename: D P
authors.initials: DP
authors.lastname: Schrum
authors.forename: C T
authors.initials: CT
authors.lastname: Culbertson
authors.forename: S C
authors.initials: SC
authors.lastname: Jacobson
authors.forename: J M
authors.initials: JM
authors.lastname: Ramsey