title: Allelic diversity and population structure of Bacillus sphaericus as revealed by multilocus sequence typing.
abstract: The genetic diversity of 35 Bacillus sphaericus strains was analyzed by a newly developed multilocus sequence typing (MLST) scheme, toxin gene pool survey, and mosquito bioassay. The results demonstrated that strains assigned to the same sequence type (ST) had the same occurrence of toxin genes. Further sequence analysis revealed that toxic strains presented a nearly clonal population structure, whereas nontoxic strains had a high level of heterogeneity and were significantly distinct from toxic strains.
authors.affiliation: Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Ge
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Hu
authors.forename: Dasheng
authors.initials: D
authors.lastname: Zheng
authors.forename: Yiming
authors.initials: Y
authors.lastname: Wu
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Yuan
mesh_terms: D000483:Alleles; D000818:Animals; D001407:Bacillus / Q000145:classification / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics; D001427:Bacterial Toxins / Q000235:genetics*; D015373:Bacterial Typing Techniques / Q000379:methods*; D001483:Base Sequence; D009033:Culicidae / Q000382:microbiology; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D014644:Genetic Variation; D058885:Multilocus Sequence Typing / Q000379:methods*; D010572:Pest Control, Biological / Q000379:methods; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004269:DNA, Bacterial; C046134:binB protein, Bacillus sphaericus