title: Isozymes from the herbivorous gecarcinid land crab, Gecarcoidea natalis that possess both lichenase and endo-β-1,4-glucanase activity.
abstract: Three isozymes with both lichenase and endo-β-1,4-glucanase activity were purified and characterised from the midgut gland of the herbivorous gecarcinid land crab, Gecarcoidea natalis. The three isozymes, termed 1a, 1b and 2, had respective molecular masses of 53 ± 0 (3), 43 ± 0 (3) and 47.4 ± 0(3) kDa. All isozymes possessed similar V(max) values and thus hydrolysed both carboxy methyl cellulose and lichenan equally. Furthermore the chromatography profiles for lichenase activities mirrored that for endo-β-1,4-glucanase activities suggesting that the same enzyme possessed both activities. Given this, the endo-β-1,4-glucanase enzymes described for other animals, may, like the isozymes described in this study, may be able to hydrolyse lichenan. However this ability needs to be confirmed. The main digestive function of these isozymes may be to hydrolyse hemicelluloses such as lichenan and mixed beta-D-glucan. All three isozymes randomly hydrolysed internal glycosidic bonds within carboxy methyl cellulose and lichenan to release short oligomers of 4-5 glucose units in length. They also hydrolysed cellotetraose to either two units of cellobiose or cellotriose and glucose. Cellotriose was hydrolysed to cellobiose and glucose. All three enzymes lacked β-1,4-glucosidase activity as they could not hydrolyse cellobiose.
authors.affiliation: School of Life and Environmental Sciences, Deakin University, Australia. stuart.linton@deakin.edu.au
authors.forename: Stuart M
authors.initials: SM
authors.lastname: Linton
authors.forename: Alicia J
authors.initials: AJ
authors.lastname: Shirley
mesh_terms: D000220:Adaptation, Biological; D000818:Animals; D003386:Brachyura / Q000201:enzymology* / Q000235:genetics; D002266:Carboxymethylcellulose Sodium / Q000737:chemistry / Q000378:metabolism; D002480:Cellulase / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D002482:Cellulose / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism; D005936:Glucans / Q000737:chemistry / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D013379:Substrate Specificity; D013780:Tetroses / Q000737:chemistry / Q000378:metabolism
chemical_list: D005936:Glucans; D007527:Isoenzymes; D013780:Tetroses; C048468:cellotetraose; D002482:Cellulose; D006026:Glycoside Hydrolases; D002480:Cellulase; C028020:licheninase; D002266:Carboxymethylcellulose Sodium; C015968:lichenin