title: Effect of medium/ω-6 long chain triglyceride-based emulsion on leucocyte death and inflammatory gene expression.
abstract: Lipid emulsion (LE) containing medium/ω-6 long chain triglyceride-based emulsion (MCT/ω-6 LCT LE) has been recommended in the place of ω-6 LCT-based emulsion to prevent impairment of immune function. The impact of MCT/ω-6 LCT LE on lymphocyte and neutrophil death and expression of genes related to inflammation was investigated. Seven volunteers were recruited and infusion of MCT/ω-6 LCT LE was performed for 6 h. Four volunteers received saline and no change was found. Blood samples were collected before, immediately afterwards and 18 h after LE infusion. Lymphocytes and neutrophils were studied immediately after isolation and after 24 and 48 h in culture. The following determinations were carried out: plasma-free fatty acids, triacylglycerol and cholesterol concentrations, plasma fatty acid composition, neutral lipid accumulation in lymphocytes and neutrophils, signs of lymphocyte and neutrophil death and lymphocyte expression of genes related to inflammation. MCT/ω-6 LCT LE induced lymphocyte and neutrophil death. The mechanism for MCT/ω-6 LCT LE-dependent induction of leucocyte death may involve changes in neutral lipid content and modulation of expression of genes related to cell death, proteolysis, cell signalling, inflammatory response, oxidative stress and transcription.
authors.affiliation: Institute of Physical Activity and Sports Sciences, Cruzeiro do Sul University, São Paulo, SP, Brazil. maria.boaventura@cruzeirodosul.edu.br
authors.forename: M F
authors.initials: MF
authors.lastname: Cury-Boaventura
authors.forename: R
authors.initials: R
authors.lastname: Gorjão
authors.forename: T
authors.initials: T
authors.lastname: Martins de Lima
authors.forename: J
authors.initials: J
authors.lastname: Fiamoncini
authors.forename: A B P
authors.initials: AB
authors.lastname: Godoy
authors.forename: F C
authors.initials: FC
authors.lastname: Deschamphs
authors.forename: F G
authors.initials: FG
authors.lastname: Soriano
authors.forename: R
authors.initials: R
authors.lastname: Curi
mesh_terms: D000328:Adult; D017209:Apoptosis / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002784:Cholesterol / Q000097:blood; D053938:DNA Fragmentation; D003652:Decanoic Acids / Q000097:blood; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics; D005217:Fat Emulsions, Intravenous / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D005230:Fatty Acids, Nonesterified / Q000032:analysis / Q000097:blood; D043371:Fatty Acids, Omega-6 / Q000097:blood / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D007249:Inflammation / Q000235:genetics*; D007958:Leukocyte Count; D007962:Leukocytes / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D009336:Necrosis / Q000139:chemically induced / Q000473:pathology; D009504:Neutrophils / Q000187:drug effects / Q000473:pathology; D010169:Palmitic Acids / Q000097:blood; D013229:Stearic Acids / Q000097:blood; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014280:Triglycerides / Q000097:blood / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D015854:Up-Regulation / Q000187:drug effects / Q000235:genetics; D055815:Young Adult
chemical_list: D003652:Decanoic Acids; D005217:Fat Emulsions, Intravenous; D005230:Fatty Acids, Nonesterified; D043371:Fatty Acids, Omega-6; D010169:Palmitic Acids; D013229:Stearic Acids; D017392:Thiobarbituric Acid Reactive Substances; D014280:Triglycerides; D002784:Cholesterol