title: Sesquiterpene glycosides from Cosmospora joca.
abstract: Five novel sesquiterpene glycosides, namely, cosmosporasides A-E (1-5), were isolated from the ethyl acetate extract of the fermented broth of Cosmospora joca. The structures of 1-5 were elucidated on the basis of spectroscopic data analyses, monosaccharide composition analyses, and monosaccharide chirality analyses. The relative configuration of the sesquiterpene moiety of 3-5 was determined by J-based configuration analyses and supported by NOESY assignments. The inhibitory effects of 1-5 on the nitric oxide (NO) production in lipopolysaccharide (LPS)-activated murine macrophage RAW264.7 cells were evaluated; all except 3 inhibited NO production at 100 μM. 4 was the most potent, with an average maximum inhibition and a median inhibitory concentration value of 71.70 ± 1.23% and 65.98 ± 0.53 μM, respectively.
authors.affiliation: Graduate Institute of Pharmacognosy, Taipei Medical University, Taipei, Taiwan 110, Republic of China. thlee@tmu.edu.tw
authors.forename: Tzong-Huei
authors.initials: TH
authors.lastname: Lee
authors.forename: Chung-Kuang
authors.initials: CK
authors.lastname: Lu
authors.forename: Guei-Jane
authors.initials: GJ
authors.lastname: Wang
authors.forename: Ya-Chih
authors.initials: YC
authors.lastname: Chang
authors.forename: Wen-Bin
authors.initials: WB
authors.lastname: Yang
authors.forename: Yu-Min
authors.initials: YM
authors.lastname: Ju
mesh_terms: D000818:Animals; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006999:Hypocreales / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis; D052247:Nitric Oxide Synthase Type II / Q000037:antagonists & inhibitors; D019906:Nuclear Magnetic Resonance, Biomolecular; D018517:Plant Roots / Q000737:chemistry; D012717:Sesquiterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D013624:Taiwan
chemical_list: D006027:Glycosides; D008070:Lipopolysaccharides; D012717:Sesquiterpenes; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II