title: Pathogen detection using short-RNA deep sequencing subtraction and assembly.
abstract: Early and accurate detection of human pathogen infection is critical for treatment and therapeutics. Here we describe pathogen identification using short RNA subtraction and assembly (SRSA), a detection method that overcomes the requirement of prior knowledge and culturing of pathogens, by using degraded small RNA and deep sequencing technology. We prove our approach's efficiency through identification of a combined viral and bacterial infection in human cells.
authors.affiliation: Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv 69978, Israel.
authors.forename: Ofer
authors.initials: O
authors.lastname: Isakov
authors.forename: Shira
authors.initials: S
authors.lastname: Modai
authors.forename: Noam
authors.initials: N
authors.lastname: Shomron
mesh_terms: D002460:Cell Line; D006678:HIV / Q000235:genetics; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D009174:Mycoplasma / Q000235:genetics; D012329:RNA, Bacterial / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D012680:Sensitivity and Specificity; D016415:Sequence Alignment; D017423:Sequence Analysis, RNA / Q000379:methods*
chemical_list: D012329:RNA, Bacterial; D012367:RNA, Viral