title: The GABA(A)ρ receptors in hippocampal spontaneous activity and their distribution in hippocampus, amygdala and visual cortex.
abstract: A bicuculline-resistant and TPMPA-sensitive GABAergic component was identified in hippocampal neurons in culture and in acute isolated brain slices. In both preparations, total GABAergic activity showed two inactivation kinetics: fast and slow. RT-PCR, in situ hybridization (ISH) and immunohistochemistry detected expression of GABAρ subunits. Immunogold and electron microscopy indicated that the receptors are mostly extrasynaptic. In addition, by RT-PCR and immunofluorescence we found GABAρ present in amygdala and visual cortex.
authors.affiliation: Departamento de Neurobiología Celular y Molecular, Laboratorio de Neurobiología Molecular y Celular, Instituto de Neurobiología, Campus UNAM-Juriquilla, Querétaro, QRO 76230, Mexico.
authors.forename: Abraham
authors.initials: A
authors.lastname: Rosas-Arellano
authors.forename: Jorge
authors.initials: J
authors.lastname: Parodi
authors.forename: Arturo I
authors.initials: AI
authors.lastname: Machuca-Parra
authors.forename: Adriana
authors.initials: A
authors.lastname: Sánchez-Gutiérrez
authors.forename: Nibaldo C
authors.initials: NC
authors.lastname: Inestrosa
authors.forename: Ricardo
authors.initials: R
authors.lastname: Miledi
authors.forename: Ataúlfo
authors.initials: A
authors.lastname: Martínez-Torres
mesh_terms: D000679:Amygdala / Q000502:physiology*; D000818:Animals; D004625:Embryo, Nonmammalian / Q000166:cytology; D006624:Hippocampus / Q000166:cytology / Q000502:physiology*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D066298:In Vitro Techniques; D051379:Mice; D008810:Mice, Inbred C57BL; D055369:Miniature Postsynaptic Potentials; D009474:Neurons / Q000502:physiology*; D018408:Patch-Clamp Techniques; D021122:Protein Subunits / Q000502:physiology; D017966:Pyramidal Cells / Q000502:physiology; D011963:Receptors, GABA-A / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013569:Synapses / Q000502:physiology; D014793:Visual Cortex / Q000502:physiology*
chemical_list: D021122:Protein Subunits; D011963:Receptors, GABA-A