title: Analysis of the heterochromatin of Cebus (Primates, Platyrrhini) by micro-FISH and banding pattern comparisons.
abstract: The karyotype of the neotropical primate genus Cebus (Platyrrhini: Cebidae), considered the most ancestral one, shows the greatest amount of heterochromatin described among Platyrrhini genera. Banding techniques and restriction enzyme digestion have previously revealed great variability of quantity and composition of heterochromatin in this genus. In this context, we use fluorescence in situ hybridization (FISH) to analyse this genomic region and discuss its possible role in the diversification of Cebus.We used a heterochromatin probe for chromosome 11 of Cebus libidinosus (11qHe+ CLI probe), obtained by chromosome microdissection. Twenty-six specimens belonging to the families Atelidae, Cebidae, Callitrichidae and Pithecidae (Platyrrhini) were studied. Fourteen out of 26 specimens were Cebus (Cebidae) individuals of C. libidinosus, C. xanthosternos, C. apella, C. nigritus, C. albifrons, C. kaapori and C. olivaceus. In Cebus specimens, we found 6 to 22 positive signals located in interstitial and telomeric positions along the different species. No hybridization signal was observed among the remaining Ceboidea species, thus reinforcing the idea of a Cebus-specific heterochromatin composed of a complex system of repetitive sequences.
authors.affiliation: Grupo de Investigación en Biología Evolutiva, Laboratorio 46, 4° piso, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón II - Ciudad Universitaria, Intendente Güiraldes 2160 - C1428EGA, Ciudad Autónoma de Buenos Aires, Argentina. mnieves@ege.fcen.uba.ar
authors.forename: Mariela
authors.initials: M
authors.lastname: Nieves
authors.forename: Edivaldo H C
authors.initials: EH
authors.lastname: De Oliveira
authors.forename: Paulo J S
authors.initials: PJ
authors.lastname: Amaral
authors.forename: Cleusa Y
authors.initials: CY
authors.lastname: Nagamachi
authors.forename: Julio C
authors.initials: JC
authors.lastname: Pieczarka
authors.forename: María C
authors.initials: MC
authors.lastname: Mühlmann
authors.forename: Marta D
authors.initials: MD
authors.lastname: Mudry
mesh_terms: D000818:Animals; D002428:Cebus / Q000235:genetics*; D002871:Chromosome Banding; D002875:Chromosomes / Q000235:genetics*; D006570:Heterochromatin / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D042282:Microdissection; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D006570:Heterochromatin