title: A novel dermal substitute based on biofunctionalized electrospun PCL nanofibrous matrix.
abstract: In this study, nanofibrous matrices of polycaprolactone (PCL) and PCL/collagen with immobilized epidermal growth factor (EGF) were successfully fabricated by electrospinning for the purpose of damaged skin regeneration. Nanofiber diameters were found to be 284 ± 48 nm for PCL and 330 ± 104 nm for PCL/collagen matrices. The porosities were calculated as 85% for PCL and 90% for PCL/collagen matrices. The covalent immobilization of EGF onto the nanofibrous matrices was verified by the increase of surface atomic nitrogen ratio from 1.0 to 2.4% for PCL and from 3.7 to 4.7% for PCL/collagen. Moreover, EGF immobilization efficiencies of PCL and PCL/collagen matrices were determined as 98.5 and 99.2%, respectively. Human dermal keratinocytes (HS2) were cultivated on both neat and EGF immobilized PCL and PCL/collagen matrices to investigate the effects of matrix chemical composition and presence of EGF on cell proliferation and differentiation. EGF immobilized PCL/collagen matrices exerted early cell spreading and rapid proliferation. Statistically high expression levels of loricrin in HS2 cells cultivated on EGF immobilized PCL/collagen matrices were (p < 0.001) regarding superior differentiation ability of these cells compared to HS2 cells cultured on neat PCL and PCL/collagen matrices. In conclusion, this novel EGF immobilized PCL/collagen nanofibrous matrix could potentially be considered as an alternative dermal substitutes and wound healing material for skin tissue engineering applications.
authors.affiliation: Department of Bioengineering, Hacettepe University, Ankara, Turkey. menemse@hacettepe.edu.tr
authors.forename: Menemşe
authors.initials: M
authors.lastname: Gümüşderelioğlu
authors.forename: Sevcan
authors.initials: S
authors.lastname: Dalkıranoğlu
authors.forename: R Seda Tığlı
authors.initials: RS
authors.lastname: Aydın
authors.forename: Soner
authors.initials: S
authors.lastname: Cakmak
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D003094:Collagen / Q000737:chemistry; D004815:Epidermal Growth Factor / Q000008:administration & dosage*; D005786:Gene Expression Regulation; D006801:Humans; D015603:Keratinocytes / Q000166:cytology* / Q000378:metabolism; D057139:Nanofibers / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry*; D016062:Porosity; D017768:Skin, Artificial / Q000639:trends*; D023822:Tissue Engineering
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; C016240:polycaprolactone; D004815:Epidermal Growth Factor; D003094:Collagen