title: Host derived inflammatory phospholipids regulate rahU (PA0122) gene, protein, and biofilm formation in Pseudomonas aeruginosa.
abstract: This study describes the role of "inflammatory" oxidized (Ox) phospholipids in regulation of rahU (PA0122) expression and biofilm formation in Pseudomonas aeruginosa (383) wild type (rahU(+)) and rahU mutant (rahU(-)) strains. Functional analysis of RahU protein from P. aeruginosa in presence of Ox-phospholipids show: (a) LysoPC modulates RahU gene/and protein expression in rahU(+) cells; (b) rahU promoter activity is increased by lysoPC and inhibited by PAPC, Ox-PAPC and arachidonic acid; the latter inhibitory effect can be reversed by lysoPC, which was enzymatically derived from PAPC; (c) biofilm formation increased in rahU(-) cells as compared to rahU(+); and (d) inhibition of rahU promoter activity by PAPC and AA (but not lysoPC) showed significantly augmented biofilm formation in rahU(+) but not in rahU(-) cells. This study shows that host derived Ox-phospholipids affect P. aeruginosa-rahU gene and protein expression, which in turn modulates biofilm formation. The accompanying paper describes the role of RahU protein in eukaryotic-host cells.
authors.affiliation: Research Department, Carilion Clinic and Virginia Tech Carilion School of Medicine, Roanoke, VA 24013, USA.
authors.forename: Jayasimha
authors.initials: J
authors.lastname: Rao
authors.forename: Antonio
authors.initials: A
authors.lastname: DiGiandomenico
authors.forename: Mykhaylo
authors.initials: M
authors.lastname: Artamonov
authors.forename: Norbert
authors.initials: N
authors.lastname: Leitinger
authors.forename: Ashok R
authors.initials: AR
authors.lastname: Amin
authors.forename: Joanna B
authors.initials: JB
authors.lastname: Goldberg
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology; D018441:Biofilms / Q000254:growth & development; D015870:Gene Expression; D005798:Genes, Bacterial; D054884:Host-Pathogen Interactions / Q000235:genetics / Q000276:immunology / Q000502:physiology; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism; D008954:Models, Biological; D009154:Mutation; D010084:Oxidation-Reduction; D010743:Phospholipids / Q000378:metabolism*; D011552:Pseudomonas Infections / Q000276:immunology / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity* / Q000502:physiology
chemical_list: D001426:Bacterial Proteins; D018836:Inflammation Mediators; D010743:Phospholipids