title: Development and validation for identity testing of I-DNADuo, a combination of I-DNA1 and a new multiplex system, I-DNA2.
abstract: The I-DNADuo multiplex system combination is composed of previously validated I-DNA1 and a new short tandem repeat (STR) multiplex named I-DNA2 that analyses 11 STR loci plus amelogenin. I-DNADuo, with amplicon sizes ranging from 57 to 298 bp, is specifically designed to analyse amelogenin and 15 STR loci (ten of them plus amelogenin in duplicate), including all the STR loci of the CODIS, ISSL and ECL databases, and seven of the eight in GCL. The validation of I-DNADuo shows that it is a highly sensitive, robust multiplex system for obtaining individual genetic profiles and for detecting and preventing allelic dropouts.
authors.affiliation: BIOMICs Research Group, Centre for Research and Advanced Studies-CIEA Lucio Lascaray, University of the Basque Country UPV/EHU (Vitoria-Gasteiz), Vitoria-Gasteiz, Spain.
authors.forename: Adrian
authors.initials: A
authors.lastname: Odriozola
authors.forename: Jose María
authors.initials: JM
authors.lastname: Aznar
authors.forename: David
authors.initials: D
authors.lastname: Celorrio
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Bravo
authors.forename: Juan Jose
authors.initials: JJ
authors.lastname: Builes
authors.forename: Marian
authors.initials: M
authors.lastname: Martinez de Pancorbo
mesh_terms: D053523:Amelogenin / Q000235:genetics; D016172:DNA Fingerprinting; D030541:Databases, Genetic; D053803:Forensic Genetics; D056426:Genetic Loci; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics*; D060885:Multiplex Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D053523:Amelogenin