title: Genetic characterization of multidrug-resistant, extended-spectrum- β-lactamase-producing Vibrio cholerae O1 outbreak strains, Mpumalanga, South Africa, 2008.
abstract: Thirty-one antimicrobial-resistant, extended-spectrum-β-lactamase-producing strains of Vibrio cholerae O1 serotype Ogawa associated with an outbreak of cholera in South Africa (2008) were investigated. Ten selected cholera strains were PCR positive for the SXT element, harbored mutations in the quinolone resistance-determining regions of GyrA (Ser83-Ile) and ParC (Ser85-Leu), and produced TEM-63 β-lactamase.
authors.affiliation: Enteric Diseases Reference Unit, National Institute for Communicable Diseases, National Health Laboratory Service, Private Bag X4, Sandringham, 2131 Johannesburg, South Africa.
authors.forename: Husna
authors.initials: H
authors.lastname: Ismail
authors.forename: Anthony M
authors.initials: AM
authors.lastname: Smith
authors.forename: Arvinda
authors.initials: A
authors.lastname: Sooka
authors.forename: Karen H
authors.initials: KH
authors.lastname: Keddy
mesh_terms: D002771:Cholera / Q000453:epidemiology* / Q000382:microbiology*; D027081:DNA Gyrase / Q000235:genetics; D027101:DNA Topoisomerase IV / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004196:Disease Outbreaks; D024901:Drug Resistance, Multiple, Bacterial; D006801:Humans; D016133:Polymerase Chain Reaction; D013019:South Africa / Q000453:epidemiology; D041241:Vibrio cholerae O1 / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; C440929:beta-lactamase TEM-63; D001618:beta-Lactamases; D027101:DNA Topoisomerase IV; D027081:DNA Gyrase