title: Early response of mechanically exposed dental pulps of swine to antibacterial-hemostatic agents or diode laser irradiation.
abstract: OBJECTIVE
  The purpose of this study was to compare the effectiveness of an antibacterial and hemostatic agent to diode laser irradiation in the healing of mechanically exposed porcine pulps.
  
  
  METHODS
  The experiment required three adult swine (Sus scrofa domestica, Yorkshire) with 36 teeth prepared with occlusal penetrations into the pulpal tissues. The preparations were performed under general anesthesia and the pulps were exposed using high speed instrumentation with rubber dam isolation and a disinfected field. Following instrumentation the coronal pulpal tissue was amputated and immediately treated with ferric sulfate and chlorhexidine semi-gel (12), diluted Buckley' formocresol solution (12) for 5 minutes or laser irradiation with a diode laser (12). After treatment, hemostasis was obtained and a ZOE base applied to the treated pulps (36). The pulpal bases were all covered with a RMGI (Fuji II LC). The tissue samples were collected at 4 weeks (28 days). Following fixation, the samples were de-mineralized, sectioned, stained and histologically graded with a scale of 0-4.
  
  
  RESULTS
  The treatment groups were statistically different with the Laser Treated Group demonstrating the least inflammation.
  
  
  CONCLUSIONS
  Pulpotomy treatment with the KaVo Gentle Ray Diode Laser demonstrated significantly less inflammation than the other two pulpal therapy modalities. The ferric sulfate and chlorhexidine mixture demonstrated the greatest inflammation as histologically graded. Also, the histological sections of pulpotomized swine teeth treated with the ferric sulfate and chlorhexidine mixture presented with black pigmented areas in the pulp and surrounding tissue. The formocresol group (clinical standard) and the diode laser group did not present with the black precipitate.
authors.affiliation: Northwestern University, Children's Medical Center, Chicago USA. cannon.m@comcast.net
authors.forename: M
authors.initials: M
authors.lastname: Cannon
authors.forename: C
authors.initials: C
authors.lastname: Wagner
authors.forename: J Z
authors.initials: JZ
authors.lastname: Thobaben
authors.forename: R
authors.initials: R
authors.lastname: Jurado
authors.forename: D
authors.initials: D
authors.lastname: Solt
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000627:therapeutic use*; D001672:Biocompatible Materials / Q000627:therapeutic use; D002710:Chlorhexidine / Q000627:therapeutic use; D003782:Dental Pulp / Q000187:drug effects / Q000528:radiation effects*; D003785:Dental Pulp Capping / Q000379:methods; D003789:Dental Pulp Exposure / Q000188:drug therapy / Q000532:radiotherapy / Q000628:therapy*; D004338:Drug Combinations; D005290:Ferric Compounds / Q000627:therapeutic use; D005566:Formocresols / Q000627:therapeutic use; D005899:Glass Ionomer Cements / Q000627:therapeutic use; D006487:Hemostasis / Q000187:drug effects / Q000528:radiation effects; D006490:Hemostatics / Q000627:therapeutic use*; D053685:Laser Therapy / Q000379:methods*; D054023:Lasers, Semiconductor / Q000627:therapeutic use*; D010858:Pigmentation / Q000187:drug effects; D011672:Pulpotomy / Q000379:methods; D012387:Root Canal Filling Materials / Q000627:therapeutic use; D013552:Swine; D015035:Zinc Oxide-Eugenol Cement / Q000627:therapeutic use
chemical_list: D000890:Anti-Infective Agents; D001672:Biocompatible Materials; D004338:Drug Combinations; D005290:Ferric Compounds; D005566:Formocresols; D005899:Glass Ionomer Cements; D006490:Hemostatics; D012387:Root Canal Filling Materials; D015035:Zinc Oxide-Eugenol Cement; C031025:formocresol; C024823:ferric sulfate; D002710:Chlorhexidine