title: Conversion for Avicel and AFEX pretreated corn stover by Clostridium thermocellum and simultaneous saccharification and fermentation: insights into microbial conversion of pretreated cellulosic biomass.
abstract: In this study, efforts were taken to compare solubilization of Avicel and AFEX pretreated corn stover (AFEX CS) by SSF and Clostridium thermocellum fermentation, with an aim to gain insights into microbial conversion of pretreated cellulosic biomass. Solubilization rates for AFEX CS are comparable for the two systems while solubilization of Avicel is much faster by C. thermocellum. Initial catalyst loading impacts final cellulose conversion for SSF but not for C. thermocellum. Hydrolysis of the two substrates using cell-free C. thermocellum fermentation broth revealed much smaller difference in cellulose conversion than the difference observed for growing cultures. Tests on hemicellulose removal and particle size reduction for AFEX CS indicated that substrate accessibility is very important for enhanced solubilization by C. thermocellum.
authors.affiliation: Thayer School of Engineering at Dartmouth College, 8000 Cummings Hall, Hanover, NH 03755, USA.
authors.forename: Xiongjun
authors.initials: X
authors.lastname: Shao
authors.forename: Mingjie
authors.initials: M
authors.lastname: Jin
authors.forename: Anna
authors.initials: A
authors.lastname: Guseva
authors.forename: Chaogang
authors.initials: C
authors.lastname: Liu
authors.forename: Venkatesh
authors.initials: V
authors.lastname: Balan
authors.forename: David
authors.initials: D
authors.lastname: Hogsett
authors.forename: Bruce E
authors.initials: BE
authors.lastname: Dale
authors.forename: Lee
authors.initials: L
authors.lastname: Lynd
mesh_terms: D018533:Biomass; D050260:Carbohydrate Metabolism; D002482:Cellulose; D048013:Acetivibrio thermocellus / Q000378:metabolism*; D005285:Fermentation; D006868:Hydrolysis; D010316:Particle Size; D012995:Solubility; D003313:Zea mays / Q000378:metabolism* / Q000382:microbiology
chemical_list: D002482:Cellulose