title: A role for reactive oxygen species in endotoxin-induced elevation of MOR expression in the nervous and immune systems.
abstract: This study examined the mechanism by which exposure to lipopolysaccharide (LPS) alters mu-opioid receptor (MOR) expression in immune and neuronal cells using an in vitro conditioned medium model system. We found that LPS stimulated the intracellular accumulation of reactive oxygen species (ROS) and MOR expression in macrophage-like TPA-HL-60 cells. Conditioned medium from the LPS-stimulated TPA-HL-60 cells increased MOR expression in SH-SY5Y cells, a neuronal cell model, through actions mediated by TNF-α and GM-CSF. These data suggest that the endotoxin, LPS, modulates MOR expression in nervous and immune cells via ROS signaling, and demonstrates the crosstalk that exists within the neuroimmune axis.
authors.affiliation: Institute of NeuroImmune Pharmacology, Seton Hall University, 400 South Orange Ave., South Orange, NJ 07079, USA.
authors.forename: Erik F
authors.initials: EF
authors.lastname: Langsdorf
authors.forename: Xin
authors.initials: X
authors.lastname: Mao
authors.forename: Sulie L
authors.initials: SL
authors.lastname: Chang
mesh_terms: D004731:Endotoxins / Q000633:toxicity*; D005786:Gene Expression Regulation; D018922:HL-60 Cells; D006801:Humans; D007107:Immune System / Q000187:drug effects / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D009420:Nervous System / Q000187:drug effects / Q000378:metabolism; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D017450:Receptors, Opioid, mu / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D004731:Endotoxins; D017382:Reactive Oxygen Species; D017450:Receptors, Opioid, mu