title: Expression of response gene to complement-32 in renal tissue of children with immunoglobulin A nephropathy.
abstract: OBJECTIVE
  This study aimed to investigate the expression and significance of response gene to complement-32 (RGC-32) in renal tissue of children with immunoglobulin A nephropathy (IgAN).
  
  
  METHODS
  Forty-five patients diagnosed as having IgAN by renal biopsy were enrolled. The expression of RGC-32, α-smooth muscle actin (α-SMA) and transforming growth factor-β(1) (TGF-β(1)) was observed by immunohistostaining. The relationshis between the expression of RGC-32, α-SMA, TGF-β1, degree of renal pathological lesions in IgAN and clinical index were assessed by Spearman correlation.
  
  
  RESULTS
  Immunohistostaining analysis showed that RGC-32 protein was present in epithelial cells of renal tubules in normal and IgAN renal tissues. With more severe renal pathological lesions, the expression of RGC-32 in IgAN was increased. The expression of RGC-32 was positively correlated with that of α-SMA, TGF-β(1) and the degree of renal pathological lesions in children with IgAN (p < 0.05), but had no relationship with serum creatinine, urinary N-acetyl-β-d-glucosaminidase/creatinine, urinary microalbuminuria/creatinine, urinary microimmunoglobulin/creatinine or urinary α(1)-microglobulin/creatinine ratio (p > 0.05).
  
  
  CONCLUSIONS
  Expression of RGC-32 can reflect the degree of renal pathological lesions in IgAN. RGC-32 may participate in the renal tubulointerstitial lesions in children with IgAN, especially in epithelial -mesenchymal transition induced by TGF-β(1).
authors.affiliation: Department of Nephrology and Rheumatology, Children's Hospital of Fudan University, Shanghai, PR China.
authors.forename: Xiao-Ling
authors.initials: XL
authors.lastname: Niu
authors.forename: Xin-Yu
authors.initials: XY
authors.lastname: Kuang
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Zhang
authors.forename: Xue-Guang
authors.initials: XG
authors.lastname: Liu
authors.forename: Zhong-Hua
authors.initials: ZH
authors.lastname: Zhao
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Hong
authors.initials: H
authors.lastname: Xu
authors.forename: Wen-Yan
authors.initials: WY
authors.lastname: Huang
mesh_terms: D018797:Cell Cycle Proteins / Q000032:analysis / Q000096:biosynthesis*; D002648:Child; D005260:Female; D005922:Glomerulonephritis, IGA / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D009124:Muscle Proteins / Q000032:analysis / Q000096:biosynthesis*; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000096:biosynthesis*
chemical_list: D018797:Cell Cycle Proteins; D009124:Muscle Proteins; D009419:Nerve Tissue Proteins; C115049:RGCC protein, human