title: Unsterile and continuous production of polyhydroxybutyrate by Halomonas TD01.
abstract: An unsterile and continuous fermentation process was developed based on a halophilic bacterium termed Halomonas TD01 isolated from a salt lake in Xinjiang, China. The strain reached 80 g/L cell dry weight containing 80% poly(3-hydroxybutyrate) (PHB) on glucose salt medium during a 56 h fed-batch process. In a 14-day open unsterile and continuous process, the cells grew to an average of 40 g/L cell dry weight containing 60% PHB in the first fermentor with glucose salt medium. Continuous pumping of cultures from the first fermentor to the second fermentor containing the nitrogen-deficient glucose salt medium diluted the cells but allowed them to maintain a PHB level of between 65% and 70% of cell dry weight. Glucose to PHB conversions were between 20% and 30% in the first fermentor and above 50% in the second one. This unsterile and continuous fermentation process opens a new area for reducing the cost in polyhydroxyalkanoates production.
authors.affiliation: MOE Key Laboratory of Protein Sciences, Department of Biological Sciences and Biotechnology, School of Life Sciences, Tsinghua University, Beijing 100084, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Tan
authors.forename: Yuan-Sheng
authors.initials: YS
authors.lastname: Xue
authors.forename: Gulsimay
authors.initials: G
authors.lastname: Aibaidula
authors.forename: Guo-Qiang
authors.initials: GQ
authors.lastname: Chen
mesh_terms: D001483:Base Sequence; D002850:Chromatography, Gel; D017931:DNA Primers; D005240:Feasibility Studies; D005285:Fermentation; D020632:Halomonas / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D006885:Hydroxybutyrates / Q000378:metabolism*; D046529:Microscopy, Electron, Transmission; D008970:Molecular Weight; D010802:Phylogeny; D011108:Polymers / Q000378:metabolism*; D014871:Water Microbiology
chemical_list: D017931:DNA Primers; D006885:Hydroxybutyrates; D011108:Polymers