title: Osteogenic and angiogenic effects of mesenchymal stromal cells with co-transfected human Ang-1 gene and BMP2 gene.
abstract: To increase the osteogenic and angiogenic effects of marrow-derived mesenchymal stromal cells (MSCs), we co-transfected (by means of lentivirus) the human angiopoietin-1 gene (hAng-1) and human bone morphogenetic protein 2 gene (hBMP2) into MSCs. Real-time PCR and ELISA showed that both genes were successfully co-expressed in the MSCs with expression sustained until the eighth week. The alkaline phosphatase activity of the MSCs was more significantly augmented by the co-transfection with both genes than by any single gene transfection. These results demonstrate that the combined gene therapy with hAng-1 and hBMP2 using lentivirally co-transfected MSCs is feasible.
authors.affiliation: Department of Orthopaedic Surgery, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 200233, People's Republic of China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Liu
authors.forename: Bingfang
authors.initials: B
authors.lastname: Zeng
authors.forename: Changqing
authors.initials: C
authors.lastname: Zhang
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000704:Analysis of Variance; D042683:Angiopoietin-1 / Q000096:biosynthesis / Q000235:genetics*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D055396:Bone Morphogenetic Protein 2 / Q000096:biosynthesis / Q000235:genetics*; D045744:Cell Line, Tumor; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D018919:Neovascularization, Physiologic; D010012:Osteogenesis; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D014162:Transfection
chemical_list: C467630:ANGPT1 protein, human; D042683:Angiopoietin-1; C525718:BMP2 protein, human; D015415:Biomarkers; D055396:Bone Morphogenetic Protein 2; D012333:RNA, Messenger; D000469:Alkaline Phosphatase