title: BRCA1, BRCA2, AR and IGF-I expression in prostate cancer: correlation between RT-qPCR and immunohistochemical detection.
abstract: Identification and characterization of biomarkers in prostate cancer are important for improving the diagnosis. The aim of this study was to determine differences in the expression of 4 genes according to the stage of malignancy in prostate cancer. We analyzed BRCA1, BRCA2, androgen receptor (AR) and IGF-I gene expression in a cohort of 98 prostate biopsies. We used TaqMan RT-qPCR for mRNA detection, and correlation with proteins was performed using immunohistochemistry. Among the 98 studied prostate biopsies, high heterogeneity in the expression of the 4 genes was detected among the different histological types. However, down-regulation of BRCA1 and BRCA2 mRNA was detected, particularly in the normal tissues. The expression of AR was dependent on the stage of the tumor. The IGF-I gene was specifically expressed in the tumor tissues. Upon comparison between protein and mRNA expression for BRCA1, BRCA2 and AR, we obtained a trend; however, this did not achieve statistical significance. Regarding IGF-I, a correlation between mRNA expression and staining intensity of the protein was found to be significant (p<0.012). The AR biomarker was found to be slightly correlated with the prostate cancer diagnosis (p=0.013). AR was found to be decreased in the tumors with a 43% sensitivity and 90% specificity. The relative risk of 2.05 (1.13-3.69) indicated a 2‑fold higher chance of cancer occurrence when AR was ≤0.206.
authors.affiliation: Centre Jean Perrin, Département d'Oncogénétique, EA 4233 Nutrition, Cancérogenèse et Thérapie Anti-tumorale, Centre Biomédical de Recherche et de Valorisation, 28 place Henri Dunant, B.P. 38, 63001 Clermont-Ferrand, France.
authors.forename: Nadège
authors.initials: N
authors.lastname: Rabiau
authors.forename: Pierre
authors.initials: P
authors.lastname: Déchelotte
authors.forename: Mawussi
authors.initials: M
authors.lastname: Adjakly
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Kemeny
authors.forename: Laurent
authors.initials: L
authors.lastname: Guy
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Boiteux
authors.forename: Fabrice
authors.initials: F
authors.lastname: Kwiatkowski
authors.forename: Yves-Jean
authors.initials: YJ
authors.lastname: Bignon
authors.forename: Dominique
authors.initials: D
authors.lastname: Bernard-Gallon
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism; D000368:Aged; D019313:BRCA1 Protein / Q000235:genetics* / Q000378:metabolism; D024682:BRCA2 Protein / Q000235:genetics* / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods; D007334:Insulin-Like Growth Factor I / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D017430:Prostate-Specific Antigen / Q000097:blood; D019048:Prostatic Intraepithelial Neoplasia / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism; D011944:Receptors, Androgen / Q000235:genetics* / Q000378:metabolism
chemical_list: D019313:BRCA1 Protein; C492913:BRCA1 protein, human; D024682:BRCA2 Protein; C551750:BRCA2 protein, human; D014408:Biomarkers, Tumor; D011944:Receptors, Androgen; D007334:Insulin-Like Growth Factor I; D017430:Prostate-Specific Antigen