title: Serpins and the complement system.
abstract: C1-inhibitor (serpin G1) is a 105 kDa inhibitor which functions as a major antiinflammatory protein in the body. It has its effects via inhibition of the proteases of the complement system and contact system of coagulation, as well as several direct effects mediated by its unique highly glycosylated N-terminal domain. The serpin controls a number of different proteases very efficiently and for some of these the function is augmented by the cofactor, heparin. Here, we describe the preparation of human plasma and recombinant C1-inhibitor and the basic methods required for their characterization, using the complement enzyme C1s as an example of a target enzyme.
authors.affiliation: Institute of Enzymology, Biological Research Center, Hungarian Academy of Sciences, Hungary.
authors.forename: László
authors.initials: L
authors.lastname: Beinrohr
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Murray-Rust
authors.forename: Leanne
authors.initials: L
authors.lastname: Dyksterhuis
authors.forename: Péter
authors.initials: P
authors.lastname: Závodszky
authors.forename: Péter
authors.initials: P
authors.lastname: Gál
authors.forename: Robert N
authors.initials: RN
authors.lastname: Pike
authors.forename: Lakshmi C
authors.initials: LC
authors.lastname: Wijeyewickrema
mesh_terms: D003174:Complement C1 Inactivator Proteins / Q000235:genetics / Q000378:metabolism*; D050718:Complement C1 Inhibitor Protein; D004591:Electrophoresis, Polyacrylamide Gel; D006493:Heparin / Q000378:metabolism; D006801:Humans; D010843:Pichia / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D003174:Complement C1 Inactivator Proteins; D050718:Complement C1 Inhibitor Protein; D011994:Recombinant Proteins; C469952:SERPING1 protein, human; D006493:Heparin