title: Distribution and molecular characterization of β-glucans from hull-less barley bran, shorts and flour.
abstract: Six hull-less barley cultivars widely grown in China were roller-milled to produce bran, shorts and flour fractions. The distribution and molecular characteristics of β-glucans from the three roller-milled fractions were investigated. The β-glucan contents in the six hull-less barley cultivars varied from 4.96% to 7.62%. For all the six cultivars, the shorts fraction contained the highest concentration of β-glucan (8.12-13.01%), followed by bran (6.15-7.58%) and flour (2.48-2.95%). Crude β-glucans were prepared from the three roller-milled fractions using aqueous sodium carbonate (pH 10). These preparations contained 45.38-71.41% β-glucan, 10.81-17.26% arabinoxylan, 2.6-9.6% protein, 2.7-9.0% starch, and 5.23-9.68% ash. Purification using α-amylase and β-xylanase in combination with pH adjustment and dialysis produced high purity β-glucan preparations (91-95%). The molecular weight (Mw) of β-glucan preparations from roller-milled fractions ranged from 117,600 to 852,400 g/mol. β-Glucan from flour had higher Mw than those from shorts and bran within the same cultivar, and β-glucan preparations from bran had the lowest Mw.
authors.affiliation: Grain College, Henan University of Technology, Zhengzhou, 450052, China; E-Mail: lilimin1970@126.com.
authors.forename: Xueling
authors.initials: X
authors.lastname: Zheng
authors.forename: Limin
authors.initials: L
authors.lastname: Li
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
mesh_terms: D000681:Amylases / Q000378:metabolism; D002254:Carbonates / Q000737:chemistry; D004043:Dietary Fiber / Q000032:analysis; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism; D005433:Flour / Q000032:analysis; D001467:Hordeum / Q000737:chemistry / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D013213:Starch / Q000737:chemistry; D014990:Xylans / Q000737:chemistry; D047071:beta-Glucans / Q000737:chemistry* / Q000378:metabolism
chemical_list: D002254:Carbonates; D004043:Dietary Fiber; D014990:Xylans; D047071:beta-Glucans; C005686:sodium carbonate; D013213:Starch; C085118:arabinoxylan; D000681:Amylases; D043364:Endo-1,4-beta Xylanases