title: Determination of palonosetron in human urine by LC-MS/MS.
abstract: BACKGROUND
  Palonosetron is used for the prevention of chemotherapy-induced nausea and vomiting. However, quantification of this drug in human urine has been rare.
  
  
  RESULTS
  A one-step dilution method for the analysis of palonosetron in human urine using LC coupled to positive MS/MS has been developed and validated according to US FDA guidelines. The method uses 200 µl of urine and covers a working range from 2.5-1000 ng/ml with a LLOQ of 2.5 ng/ml.
  
  
  CONCLUSIONS
  This new LC-MS/MS assay is sensitive and specific despite using an external standard method. It is suitable for clinical studies of palonosetron.
authors.affiliation: Department of Pharmacy, The First Affiliated Hospital with Nanjing Medical University, Nanjing, China. wangyy1978@gmail.com
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Wang
authors.forename: Yongqing
authors.initials: Y
authors.lastname: Wang
authors.forename: Peipei
authors.initials: P
authors.lastname: Zhang
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Lu
authors.forename: Ying
authors.initials: Y
authors.lastname: Gu
authors.forename: Ning
authors.initials: N
authors.lastname: Ou
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods; D006801:Humans; D007546:Isoquinolines / Q000652:urine*; D000077924:Palonosetron; D011812:Quinuclidines / Q000652:urine*; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D007546:Isoquinolines; D011812:Quinuclidines; D000077924:Palonosetron