title: Supramolecular assembled nanogel made of mannan.
abstract: The supramolecular assembly of amphiphilic mannan, synthesized by the Michael addition of hydrophobic 1-hexadecanethiol to vinyl methacrylated mannan, originates in aqueous medium the formation of a nanogel, stabilized by hydrophobic interactions among alkyl chains. The critical aggregation concentration, calculated by fluorescence spectroscopy ranged between 0.002 and 0.01 mg/mL, depending on the polymer degree of substitution. The cryo-field emission scanning electron microscopy showed spherical macromolecular micelles with diameters between 100 and 500 nm. The dynamic light scattering analysis revealed a polydisperse colloidal system, with mean hydrodynamic diameter between 50 and 140 nm, depending on the polymer degree of substitution. The nanogel is negatively charged, stable over a 6 months storage period, and stable at pH 3-8, salt or urea solutions. Bovine serum albumin and curcumin were spontaneously incorporated in the nanogel, being stabilized by the hydrophobic domains, opening the possibility for future applications as potential delivery systems for therapeutic molecules. In vitro assays were carried out to characterize the biocompatibility of the nanogel. A toxic effect of mannan-C(16) was observed, specific to mouse macrophage-like cell line J774, not affecting mouse embryo fibroblast cell line 3T3 viability.
authors.affiliation: IBB-Institute for Biotechnology and Bioengineering, Centre for Biological Engineering, University of Minho, Braga, Portugal. silviarmferreira@deb.uminho.pt
authors.forename: Sílvia A
authors.initials: SA
authors.lastname: Ferreira
authors.forename: Paula
authors.initials: P
authors.lastname: Pereira
authors.forename: Paula
authors.initials: P
authors.lastname: Sampaio
authors.forename: Paulo J G
authors.initials: PJ
authors.lastname: Coutinho
authors.forename: Francisco M
authors.initials: FM
authors.lastname: Gama
mesh_terms: D016475:3T3 Cells; D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry*; D002417:Cattle; D002460:Cell Line; D002470:Cell Survival; D003474:Curcumin / Q000008:administration & dosage; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D008351:Mannans / Q000138:chemical synthesis / Q000737:chemistry*; D051379:Mice; D000080385:Nanogels; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry*; D011094:Polyethyleneimine / Q000138:chemical synthesis / Q000737:chemistry*; D012709:Serum Albumin / Q000008:administration & dosage; D013438:Sulfhydryl Compounds / Q000138:chemical synthesis / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D001672:Biocompatible Materials; D004337:Drug Carriers; D008351:Mannans; D000080385:Nanogels; D012709:Serum Albumin; D013438:Sulfhydryl Compounds; C413692:polyethylene glycol polyethyleneimine nanogel; C086974:hexadecanethiol; D011092:Polyethylene Glycols; D011094:Polyethyleneimine; D003474:Curcumin