title: Mapping and application of enhancer-trap flippase expression in larval and adult Drosophila CNS.
abstract: The Gal4/ UAS binary method is powerful for gene and neural circuitry manipulation in Drosophila. For most neurobiological studies, however, Gal4 expression is rarely tissue-specific enough to allow for precise correlation of the circuit with behavioral readouts. To overcome this major hurdle, we recently developed the FINGR method to achieve a more restrictive Gal4 expression in the tissue of interest. The FINGR method has three components: 1) the traditional Gal4/UAS system; 2) a set of FLP/FRT-mediated Gal80 converting tools; and 3) enhancer-trap FLP (ET-FLP). Gal4 is used to define the primary neural circuitry of interest. Paring the Gal4 with a UAS-effector, such as UAS-MJD78Q or UAS-Shi(ts), regulates the neuronal activity, which is in turn manifested by alterations in the fly behavior. With an additional UAS-reporter such as UAS-GFP, the neural circuit involved in the specific behavior can be simultaneously mapped for morphological analysis. For Gal4 lines with broad expression, Gal4 expression can be restricted by using two complementary Gal80-converting tools: tub(P)>Gal80> ('flip out') and tub(P)>stop>Gal80 ('flip in'). Finally, investigators can turn Gal80 on or off, respectively, with the help of tissue-specific ET-FLP. In the flip-in mode, Gal80 will repress Gal4 expression wherever Gal4 and ET-FLP intersect. In the flip-out mode, Gal80 will relieve Gal4 repression in cells in which Gal4 and FLP overlap. Both approaches enable the restriction of the number of cells in the Gal4-defined circuitry, but in an inverse pattern. The FINGR method is compatible with the vast collection of Gal4 lines in the fly community and highly versatile for traditional clonal analysis and for neural circuit mapping. In this protocol, we demonstrate the mapping of FLP expression patterns in select ET-FLPx2 lines and the effectiveness of the FINGR method in photoreceptor cells. The principle of the FINGR method should also be applicable to other genetic model organisms in which Gal4/UAS, Gal80, and FLP/FRT are used.
authors.affiliation: Department of Zoology, University of Oklahoma - Norman, USA.
authors.forename: Taylor R
authors.initials: TR
authors.lastname: Fore
authors.forename: Audrey A
authors.initials: AA
authors.lastname: Ojwang
authors.forename: Margaret L
authors.initials: ML
authors.lastname: Warner
authors.forename: Xinyun
authors.initials: X
authors.lastname: Peng
authors.forename: Rudolf A
authors.initials: RA
authors.lastname: Bohm
authors.forename: William P
authors.initials: WP
authors.lastname: Welch
authors.forename: Lindsey K
authors.initials: LK
authors.lastname: Goodnight
authors.forename: Hong
authors.initials: H
authors.lastname: Bao
authors.forename: Bing
authors.initials: B
authors.lastname: Zhang
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D004330:Drosophila / Q000235:genetics / Q000378:metabolism*; D029721:Drosophila Proteins / Q000096:biosynthesis* / Q000235:genetics; D004742:Enhancer Elements, Genetic; D005260:Female; D005821:Genetic Techniques; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D029721:Drosophila Proteins; C557617:GAL4 protein, Drosophila; D014157:Transcription Factors