title: Y-STR genetic diversity in autochthonous Andalusians from Huelva and Granada provinces (Spain).
abstract: Seventeen Y-chromosomal short tandem repeats (STRs) were analyzed in 347 healthy, unrelated, autochthonous males from the Andalusian provinces of Huelva (N=167) and Granada (N=180). AmpFlSTR Y-filer PCR Amplification kit (Applied Biosystems) was used to type the Y-STR markers. A total of 156 and 166 different haplotypes for the 17 Y-STR set were detected in Huelva, and Granada, respectively. The same haplotype diversity was found for both samples (0.998±0.001), and the overall discrimination capacity was 0.904. The most common minimal haplotype (DYS19, DYS389 I, DYS389 II, DYS390, DYS391, DYS392, DYS393) in both subpopulations was 14-13-16-24-11-13-13, which is also the most frequent haplotype among Atlantic European populations. Comparison analysis using pairwise R(ST) values and Analysis of Molecular Variance (AMOVA) revealed a significant genetic distance between our Andalusian samples and other ones from the northern Iberian fringe (including Basque and Pyrenean populations). However, results from the multi-dimensional scaling analysis (MDS) yielded a well-defined group of Iberian populations separated from the other Mediterranean clusters observed.
authors.affiliation: Departamento de Zoología y Antropología Física, Facultad de Biología, Universidad Complutense, Ciudad Universitaria, 28040 Madrid, Spain.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Ambrosio
authors.forename: Andrea
authors.initials: A
authors.lastname: Novelletto
authors.forename: Candela
authors.initials: C
authors.lastname: Hernandez
authors.forename: Jean Michel
authors.initials: JM
authors.lastname: Dugoujon
authors.forename: César
authors.initials: C
authors.lastname: Fortes-Lima
authors.forename: Juan Nicolás
authors.initials: JN
authors.lastname: Rodriguez
authors.forename: Rosario
authors.initials: R
authors.lastname: Calderon
mesh_terms: D041322:Chromosomes, Human, Y; D016172:DNA Fingerprinting; D005006:Ethnicity / Q000235:genetics*; D014644:Genetic Variation; D005828:Genetics, Population; D006239:Haplotypes; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction; D013030:Spain