title: Piezosurgery prevents brain tissue damage: an experimental study on a new rat model.
abstract: Piezosurgery is a promising meticulous system for bone cutting, based on ultrasound microvibrations. It is thought that the impact of piezosurgery on the integrity of soft tissue is generally low, but it has not been examined critically. The authors undertook an experimental study to evaluate the brain tissue response to skull bone removal using piezosurgery compared with a conventional drilling method. In Wistar male rats, a circular bone window was drilled to the parietal bone using piezosurgery on one side and a conventional bone drill on the other side. The behavioural performance of animals was evaluated using the motor BBB test and sensory plantar test. The brains of animals were evaluated by magnetic resonance imaging (MRI) and histology. The results of MRI showed significantly increased depth and width of the brain lesion in the region of conventional drilling compared with the region where piezosurgery was used. Cresylviolet and NF 160 staining confirmed these findings. There was no significant difference in any of the behavioural tests between the two groups. In conclusion, piezosurgery is a safe method for the performance of osteotomy in close relation to soft tissue, including an extremely injury-sensitive tissue such as brain.
authors.affiliation: Department of Oral and Maxillofacial Surgery, First Faculty of Medicine, Charles University and General University Hospital, Prague, Czech Republic.
authors.forename: G
authors.initials: G
authors.lastname: Pavlíková
authors.forename: R
authors.initials: R
authors.lastname: Foltán
authors.forename: M
authors.initials: M
authors.lastname: Burian
authors.forename: E
authors.initials: E
authors.lastname: Horká
authors.forename: S
authors.initials: S
authors.lastname: Adámek
authors.forename: A
authors.initials: A
authors.lastname: Hejčl
authors.forename: T
authors.initials: T
authors.lastname: Hanzelka
authors.forename: J
authors.initials: J
authors.lastname: Sedý
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000473:pathology; D001522:Behavior, Animal / Q000502:physiology; D048588:Benzoxazines; D001921:Brain / Q000473:pathology; D001930:Brain Injuries / Q000517:prevention & control*; D004396:Coloring Agents; D006614:Hindlimb / Q000502:physiology; D007431:Intraoperative Complications / Q000517:prevention & control; D008124:Locomotion / Q000502:physiology; D008279:Magnetic Resonance Imaging; D008297:Male; D009043:Motor Activity / Q000502:physiology; D016900:Neurofilament Proteins / Q000032:analysis; D010027:Osteotomy / Q000295:instrumentation / Q000379:methods*; D010078:Oxazines; D010294:Parietal Bone / Q000601:surgery*; D059745:Piezosurgery / Q000295:instrumentation / Q000379:methods*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013697:Thermosensing / Q000502:physiology; D013997:Time Factors
chemical_list: D048588:Benzoxazines; D004396:Coloring Agents; D016900:Neurofilament Proteins; D010078:Oxazines; C061195:neurofilament protein M; C028911:cresyl violet