title: Development of magnetic multiwalled carbon nanotubes combined with near-infrared radiation-assisted desorption for the determination of tissue distribution of doxorubicin liposome injects in rats.
abstract: For the first time, magnetic multiwalled carbon nanotubes (MWNTs) combined with near-infrared radiation-assisted desorption (NIRAD) was successfully developed for the determination of tissue distribution of doxorubicin liposome injects (DOXLI) in rats. The magnetic MWNTs nanomaterials were synthesized via a simple hydrothermal process. Magnetic Fe(3)O(4) beads, with average diameters of ca. 200 nm and narrow size distribution, were decorated along MWNTs to form octopus-like nanostructures. The hybrid nanocomposites provided an efficient way for the extraction and enrichment of doxorubicin (DOX) via π-π stacking of DOX molecules onto the polyaromatic surface of MWNTs. DOX adsorbed with magnetic MWNTs could be simply and rapidly isolated through a magnetic field. In addition, due to the near-infrared radiation (NIR) absorption property of MWNTs, irradiation with NIR laser was employed to induce photothermal conversion, which could trigger rapid DOX desorption from DOX-loaded magnetic MWNTs. Extraction conditions such as amount of magnetic MWNTs added, pH values, adsorption time, desorption solvent and NIR time were investigated and optimized. Method validations including linear range, detection limit, precision, and recovery were also studied. The results showed that the proposed method based on magnetic MWNTs coupled to NIRAD was a simple, rapid and high efficient approach for the analysis of DOXLI in rat tissues.
authors.affiliation: School of Pharmacy, Fudan University, No. 826 Zhangheng Road, Shanghai 201203,China.
authors.forename: Shun
authors.initials: S
authors.lastname: Shen
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Ren
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Lu
authors.forename: Chunhui
authors.initials: C
authors.lastname: Deng
authors.forename: Xinguo
authors.initials: X
authors.lastname: Jiang
mesh_terms: D000818:Animals; D004317:Doxorubicin / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics*; D006863:Hydrogen-Ion Concentration; D007259:Infrared Rays; D007275:Injections, Intravenous; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008297:Male; D053761:Nanocomposites / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D051381:Rats; D015203:Reproducibility of Results; D014018:Tissue Distribution
chemical_list: D058185:Magnetite Nanoparticles; D037742:Nanotubes, Carbon; D004317:Doxorubicin