title: Time- and dose-dependent induction of HSP70 in Lemna minor exposed to different environmental stressors.
abstract: The objective of this study was to examine the influence of different stressors, including cadmium (heavy metal), anthracene (polycyclic aromatic hydrocarbon-PAH) and chloridazon (herbicide), on population growth and biosynthesis of cytoplasmic HSP70 in Lemna minor (duckweed) in short (4 h)- and long (7 days)-term tests. A heat shock response was confirmed in Lemna exposed to high temperature: 35, 37.5, 40, or 42.5°C in short-term (4 h) treatments. The chemicals tested stimulated the biosynthesis of the cytoplasmic HSP70 protein in a concentration-dependent way (0.5-5 μM), higher in fronds exposed to lower doses of stressors. Additionally, production of HSP70 was greater after 4 h of incubation than after 7 days. The results suggest that HSP70 could be applied as a non-specific and sensitive detector of stress induced by different chemicals at concentrations below those that produce the type of response observed in classical cytotoxicity tests, such as growth inhibition.
authors.affiliation: Department of Plant Physiology, University of Gdańsk, Gdynia, Poland. stefantukaj@gmail.com
authors.forename: Stefan
authors.initials: S
authors.lastname: Tukaj
authors.forename: Joanna
authors.initials: J
authors.lastname: Bisewska
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Roeske
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Tukaj
mesh_terms: D000873:Anthracenes / Q000633:toxicity; D029064:Araceae / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D002104:Cadmium / Q000633:toxicity; D003593:Cytoplasm / Q000187:drug effects / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004785:Environmental Pollutants / Q000737:chemistry / Q000145:classification / Q000633:toxicity*; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis / Q000378:metabolism*; D006540:Herbicides / Q000633:toxicity; D006358:Hot Temperature; D018384:Oxidative Stress / Q000187:drug effects*; D011724:Pyridazines / Q000633:toxicity; D013997:Time Factors; D018675:Toxicity Tests
chemical_list: D000873:Anthracenes; D004785:Environmental Pollutants; D018840:HSP70 Heat-Shock Proteins; D006540:Herbicides; D011724:Pyridazines; D002104:Cadmium; C005586:pyrazon; C034020:anthracene