title: CST-II's recognition domain for acceptor substrates in α-(2→8)-sialylations.
abstract: CST-II is a bacterial sialyltransferase known for its ability to perform α-(2→8)-sialylations using GM(3) related trisaccharide substrates. Previously, we probed the enzyme's substrate specificity and developed an efficient synthesis for α-(2→8)-oligosialosides, and we suggested that CST-II could have a very small substrate recognition domain. Here we report our full studies on CST-II's recognition feature for acceptor substrates. The current study further demonstrates the versatility of CST-II in preparing complex oligosaccharides that contain α-(2→8)-oligosialyl moieties.
authors.affiliation: Department of Chemistry, University of Calgary, Alberta Ingenuity Centre for Carbohydrate Science, Calgary, Canada.
authors.forename: Wenling
authors.initials: W
authors.lastname: Li
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
authors.forename: Amir J
authors.initials: AJ
authors.lastname: Zuccolo
authors.forename: Ruxiang Blake
authors.initials: RB
authors.lastname: Zheng
authors.forename: Chang-Chun
authors.initials: CC
authors.lastname: Ling
mesh_terms: D001665:Binding Sites; D002240:Carbohydrate Sequence; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry* / Q000378:metabolism*; D012799:Sialyltransferases / Q000737:chemistry* / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D009844:Oligosaccharides; D012799:Sialyltransferases