title: Genome-wide detection of meiotic DNA double-strand break hotspots using single-stranded DNA.
abstract: The controlled fragmentation of chromosomes by DNA double-strand breaks (DSBs) initiates meiotic recombination, which is essential for meiotic chromosome segregation in most eukaryotes. This chapter describes a straightforward microarray-based approach to measure the genome-wide distribution of meiotic DSBs by detecting the single-stranded DNA (ssDNA) that transiently accumulates at DSB sites during recombination. The protocol outlined here has been optimized to detect meiotic DSBs in Saccharomyces cerevisiae. However, because ssDNA is a universal intermediate of homologous recombination, this method can ostensibly be adapted to discover and analyze programmed or damage-induced DSB hotspots in other organisms whose genome sequence is available.
authors.affiliation: Whitehead Institute for Biomedical Research, Cambridge, MA, USA. blitzblau@wi.mit.edu
authors.forename: Hannah G
authors.initials: HG
authors.lastname: Blitzblau
authors.forename: Andreas
authors.initials: A
authors.lastname: Hochwagen
mesh_terms: D053903:DNA Breaks, Double-Stranded; D004277:DNA, Single-Stranded / Q000235:genetics*; D008540:Meiosis / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D012441:Saccharomyces cerevisiae / Q000235:genetics
chemical_list: D004277:DNA, Single-Stranded