title: A transgenic study on affecting potato tuber yield by expressing the rice sucrose transporter genes OsSUT5Z and OsSUT2M.
abstract: In many plants, sucrose transporters are essential for both sucrose exports from sources and imports into sinks, indicating a function in assimilate partitioning. To investigate whether sucrose transporters can improve the yield of starch plant, potato plants (Solanum tuberosum L. cv. Désirée) were transformed with cDNAs of the rice sucrose transporter genes OsSUT5Z and OsSUT2M under the control of a tuber-specific, class-I patatin promoter. Compared to the controls, the average fructose content of OsSUT5Z transgenic tubers significantly increased. However, the content of the sugars and starch in the OsSUT2M transgenic potato tubers showed no obvious difference. Correspondingly, the average tuber yield, average number of tubers per plant and average weight of single tuber showed no significant difference in OsSUT2M transgenic tubers with controls. In the OsSUT5Z transgenic lines, the average tuber yield per plant was 1.9-fold higher than the controls, and the average number of tubers per plant increased by more than 10 tubers on average, whereas the average weight of a single tuber did not increase significantly. These results suggested that the average number of tubers per plant showed more contribution than the average weight of a single tuber to the tuber yield per plant.
authors.affiliation: National Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Aijun
authors.initials: A
authors.lastname: Sun
authors.forename: Yan
authors.initials: Y
authors.lastname: Dai
authors.forename: Xinsheng
authors.initials: X
authors.lastname: Zhang
authors.forename: Chunmin
authors.initials: C
authors.lastname: Li
authors.forename: Kun
authors.initials: K
authors.lastname: Meng
authors.forename: Honglin
authors.initials: H
authors.lastname: Xu
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Wei
authors.forename: Guifang
authors.initials: G
authors.lastname: Xiao
authors.forename: Pieter B F
authors.initials: PB
authors.lastname: Ouwerkerk
authors.forename: Mei
authors.initials: M
authors.lastname: Wang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhu
mesh_terms: D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D012275:Oryza / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D035281:Plant Tubers / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism*; D011198:Solanum tuberosum / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*
chemical_list: D026901:Membrane Transport Proteins; D010940:Plant Proteins; C078729:sucrose transport protein, plant