title: Inhibitory effects of costunolide isolated from Laurus nobilis on IgE-induced degranulation of mast cell-like RBL-2H3 cells and the growth of Y16 pro-B cells.
abstract: This study investigated the inhibitory effects of costunolide isolated from the leaves of Laurus nobilis L. (Lauraceae) on basophil-mediated allergic reactions and interleukin (IL)-5-mediated B cell growth. The effects of costunolide on β-hexosaminidase (a key parameter of degranulation) release and IL-4 expression in rat basophilic leukemia (RBL-2H3) cells were determined by measuring β-hexosaminidase activity and by semi-quantitative RT-PCR, respectively. The effects of costunolide on Y16 pro-B cell viability and growth were determined by 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyltetrazolium bromide (MTT) assay. Costunolide was found significantly to inhibit β-hexosaminidase activity (p < 0.01) and IL-4 transcription in RBL-2H3 cells in a dose-dependent manner. Its 50% inhibitory concentration (IC50 ) was 34 µM, while that of the positive control, ketotifen, was 24 µM (IL-4 mRNA transcription). Moreover, costunolide dose-dependently suppressed pro-B cell growth in IL-5-stimulated Y16 cells. These results provide evidence that costunolide stabilizes mast cells by inhibiting IgE-mediated degranulation and inhibits IL-5-stimulated B cell growth.
authors.affiliation: Natural Products Research Institute, College of Pharmacy, Seoul National University, Gwanak-gu, Seoul, 151-742, Korea.
authors.forename: Tae Joon
authors.initials: TJ
authors.lastname: Kim
authors.forename: Kung-Woo
authors.initials: KW
authors.lastname: Nam
authors.forename: Bora
authors.initials: B
authors.lastname: Kim
authors.forename: Sung-Jin
authors.initials: SJ
authors.lastname: Lee
authors.forename: Ki-Bong
authors.initials: KB
authors.lastname: Oh
authors.forename: Kyeong Ho
authors.initials: KH
authors.lastname: Kim
authors.forename: Woongchon
authors.initials: W
authors.lastname: Mar
authors.forename: Jongheon
authors.initials: J
authors.lastname: Shin
mesh_terms: D000818:Animals; D015550:Cell Degranulation / Q000187:drug effects*; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D007073:Immunoglobulin E; D020128:Inhibitory Concentration 50; D015847:Interleukin-4 / Q000378:metabolism; D015848:Interleukin-5; D027442:Laurus / Q000737:chemistry*; D008407:Mast Cells / Q000187:drug effects* / Q000502:physiology; D054448:Precursor Cells, B-Lymphoid / Q000187:drug effects*; D051381:Rats; D012717:Sesquiterpenes / Q000494:pharmacology*; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism; D007783:Lactones
chemical_list: D007073:Immunoglobulin E; D015847:Interleukin-4; D015848:Interleukin-5; D012717:Sesquiterpenes; D001619:beta-N-Acetylhexosaminidases; C002602:costunolide; D007783:Lactones