title: Mitochondrial enzymes discriminate between mitochondrial disorders and chronic fatigue syndrome.
abstract: We studied the extent of mitochondrial involvement in chronic fatigue syndrome (CFS) and investigated whether measurement of mitochondrial respiratory chain complex (RCC) activities discriminates between CFS and mitochondrial disorders. Mitochondrial content was decreased in CFS compared to healthy controls, whereas RCC activities corrected for mitochondrial content were not. Conversely, mitochondrial content did not discriminate between CFS and two groups of mitochondrial disorders, whereas ATP production rate and complex I, III and IV activity did, all with higher activities in CFS. We conclude that the ATP production rate and RCC activities can reliably discriminate between mitochondrial disorders and CFS.
authors.affiliation: Neuromuscular Center Nijmegen, Department of Neurology, Radboud University Nijmegen Medical Center, Nijmegen, The Netherlands. b.smits@neuro.umcn.nl
authors.forename: Bart
authors.initials: B
authors.lastname: Smits
authors.forename: Lambert
authors.initials: L
authors.lastname: van den Heuvel
authors.forename: Hans
authors.initials: H
authors.lastname: Knoop
authors.forename: Benno
authors.initials: B
authors.lastname: Küsters
authors.forename: Antoon
authors.initials: A
authors.lastname: Janssen
authors.forename: George
authors.initials: G
authors.lastname: Borm
authors.forename: Gijs
authors.initials: G
authors.lastname: Bleijenberg
authors.forename: Richard
authors.initials: R
authors.lastname: Rodenburg
authors.forename: Baziel
authors.initials: B
authors.lastname: van Engelen
mesh_terms: D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D002950:Citrate (si)-Synthase / Q000378:metabolism; D003937:Diagnosis, Differential; D045222:Electron Transport Chain Complex Proteins / Q000378:metabolism*; D057075:Enzyme Assays; D015673:Fatigue Syndrome, Chronic / Q000175:diagnosis / Q000201:enzymology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008931:Mitochondria, Muscle / Q000201:enzymology*; D028361:Mitochondrial Diseases / Q000175:diagnosis / Q000201:enzymology*; D024101:Mitochondrial Proteins / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000378:metabolism; D055815:Young Adult
chemical_list: D045222:Electron Transport Chain Complex Proteins; D024101:Mitochondrial Proteins; D000255:Adenosine Triphosphate; D002950:Citrate (si)-Synthase