title: Acute rejection correlates with expression of major histocompatibility complex class I antigens on peripheral blood CD3(+)CD8(+) T-lymphocytes following skin transplantation in mice.
abstract: This study investigated major histocompatibility complex class I (MHC-I) antigen expression on peripheral blood T-cells after transplantation to assess its potential as an early marker of acute graft rejection (AGR). Using a mouse model with or without immunosuppressive treatment, the expression of MHC-I antigens on CD3(+)CD8(+) T-lymphocytes was assessed by flow cytometry following syngeneic graft (n = 138) or allograft (n = 138) skin transplantation. The occurrence of AGR was assessed by examining the degree of lymphocyte and monocyte infiltration in transplant biopsies. During AGR, expression of MHC-I antigens increased significantly compared with pre-transplant levels in the allograft group, even with immunosuppressive treatment. The highest expression of MHC-I antigens occurred 5 - 6 days before macroscopic rejection. These results suggest that expression of MHC-I antigens on peripheral blood CD3(+)CD8(+) T-lymphocytes could be used as an early marker for predicting AGR.
authors.affiliation: Clinical Laboratory Department, Maternity Hospital, Shandong Provincial Hospital, Shandong University, Jinan, China.
authors.forename: Y X
authors.initials: YX
authors.lastname: Zhou
authors.forename: S M
authors.initials: SM
authors.lastname: Zhao
authors.forename: N
authors.initials: N
authors.lastname: Lu
authors.forename: X J
authors.initials: XJ
authors.lastname: Yang
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.forename: Y J
authors.initials: YJ
authors.lastname: Li
authors.forename: X
authors.initials: X
authors.lastname: Zou
mesh_terms: D000818:Animals; D017252:CD3 Complex / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D005453:Fluorescence; D006084:Graft Rejection / Q000097:blood* / Q000276:immunology* / Q000473:pathology; D015395:Histocompatibility Antigens Class I / Q000097:blood*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D016038:Skin Transplantation / Q000276:immunology*
chemical_list: D017252:CD3 Complex; D015395:Histocompatibility Antigens Class I