title: Methicillin-resistant Staphylococcus aureus (MRSA) ST398 in a farmer with skin lesions and in pigs of his farm: clonal relationship and detection of lnu(A) gene.
abstract: Skin infection associated with methicillin-resistant Staphylococcus aureus (MRSA)-ST398 was detected in a pig-farmer, and MRSA-ST398 isolates were also detected in nasal samples of the patient and of 11/12 pigs on his farm. Twelve MRSA isolates were obtained from skin lesions (n = 6) and nasal samples (n = 6) of the patient in two sampling moments and 11 MRSA isolates from nasal samples of pigs. They were typed as t011-SCCmecIVa-agrI and t108-SCCmecV-agrI (patient and pigs) and t588-SCCmecV-agrI (patient). The following resistance genes were detected (number isolates): tet(K) (1), tet(L) (23), tet(M) (13), erm(A) (13), erm(C) (13), msr(A) (11), lnu(A) (21), aph(2'')-acc(6') (3), ant(4') (13), aph(3') (12), dfrS1 (15) and dfrK (22). Seventeen human and animal MRSA-ST398 isolates showed indistinguishable PFGE patterns (A1-spa-t011 or B2-spa-t108) and similar phenotypic-genotypic characteristics, including the presence of the lnu(A) gene, associated with lincomycin resistance. Potential pig-to-human transference of ST398 is suggested in this study. The first detection of the lnu(A) gene in MRSA-ST398 is reported.
authors.affiliation: Área Bioquímica y Biología Molecular, Universidad de La Rioja, Logroño, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Lozano
authors.forename: C
authors.initials: C
authors.lastname: Aspiroz
authors.forename: M
authors.initials: M
authors.lastname: Ara
authors.forename: E
authors.initials: E
authors.lastname: Gómez-Sanz
authors.forename: M
authors.initials: M
authors.lastname: Zarazaga
authors.forename: C
authors.initials: C
authors.lastname: Torres
mesh_terms: D000383:Agriculture; D000818:Animals; D015373:Bacterial Typing Techniques; D002353:Carrier State / Q000382:microbiology / Q000662:veterinary; D016000:Cluster Analysis; D016521:Electrophoresis, Gel, Pulsed-Field; D005798:Genes, Bacterial; D005838:Genotype; D006801:Humans; D008297:Male; D055624:Methicillin-Resistant Staphylococcus aureus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D008875:Middle Aged; D017720:Molecular Epidemiology; D058889:Molecular Typing; D009297:Nasal Mucosa / Q000382:microbiology; D013207:Staphylococcal Skin Infections / Q000175:diagnosis* / Q000382:microbiology*; D013552:Swine / Q000382:microbiology*; D015047:Zoonoses / Q000382:microbiology* / Q000635:transmission*