title: Effects of C-reactive protein on CC chemokine receptor 2-mediated chemotaxis of monocytes.
abstract: Periodontal infections can increase patients' serum C-reactive protein (CRP) level, which is a predictive marker of future cardiovascular events. Serum CRP may be a key mediator associating periodontitis with cardiovascular disease. It is not yet clarified whether the chemotactic activity of monocytes changes with increased serum CRP. This study investigated the influence of CRP on monocyte chemotaxis and the effects of CRP on CC chemokine receptor 2 (CCR2) expression by monocytes in vitro. Monocyte cell line THP-1 was cultured with human recombinant CRP of different final concentrations, which were 2, 4, 6, 8, and 10 mg/L, respectively. After 24 h incubation, Transwell chambers were applied to analyze the chemotactic activity of pretreated monocytes. Flow cytometry analysis and real-time reverse transcription-polymerase chain reaction (RT-PCR) were applied to detect the CCR2 protein and gene expression levels. In Transwell chambers, more cells were attracted in CRP-pretreated groups than that of blank control with no CRP (p<0.05). The chemotaxis activity was stronger in higher CRP concentration groups than lower ones (p<0.05). The CCR2 protein and mRNA expression was increased in a CRP concentration-dependent manner (p<0.05). CRP stimulation may induce CCR2 overexpression on monocytes and then promote the chemotaxis ability of monocytes. This result suggests that increased serum CRP concentration of periodontitis patients may be associated with high risk of cardiovascular disease.
authors.affiliation: State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Meng
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.forename: Lei
authors.initials: L
authors.lastname: Zhao
authors.forename: Yi-ru
authors.initials: YR
authors.lastname: Fang
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Fujimoto
authors.forename: Shun-ichiro
authors.initials: S
authors.lastname: Hirano
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Inoue
authors.forename: Kenji
authors.initials: K
authors.lastname: Uchihashi
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Nishikawa
authors.forename: Ya-fei
authors.initials: YF
authors.lastname: Wu
mesh_terms: D002097:C-Reactive Protein / Q000235:genetics / Q000494:pharmacology*; D002460:Cell Line; D002633:Chemotaxis / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D054390:Receptors, CCR2 / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D012333:RNA, Messenger; D054390:Receptors, CCR2; D011994:Recombinant Proteins; D002097:C-Reactive Protein