title: Physico-chemical characterization, antioxidant and anticancer activities in vitro of a novel polysaccharide from Melia toosendan Sieb. Et Zucc fruit.
abstract: A novel water-soluble polysaccharide pMTPS-3, obtained from Melia toosendan Sieb. Et Zucc fruit by hot-water extraction and ethanol precipitation, was fractionated by DEAE-52 cellulose anion-exchange and Sephadex G-100 gel filtration chromatography. Its primary structural features and molecular weight were characterized by Fourier infrared spectrometry (FTIR), gel permeation chromatography (GPC) and gas chromatography (GC). And the antioxidant activities of pMTPS-3 in vitro were evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay, superoxide radical scavenging assay and hydroxyl radical scavenging assay. The results suggested that pMTPS-3 was a heteropolysaccharide, composed of arabinose, glucose, mannose, and galactose in the molar ratio of 17.3:28.3:41.6:12.6 with molecular weight 26100Da. The purified pMTPS-3 was revealed to have notable scavenging activity against DPPH radical in a concentration-dependent manner and present a moderate inhibition of superoxide radicals with an IC(50) (5.6mg/ml), and potent inhibiting power for hydroxyl radical compared with crude polysaccharide. Further, it exhibited strong inhibition effect in vitro on the growth of human gastric cancer BGC-823 cells. It is strongly evidenced that pMTPS-3 purified from the crude polysaccharides of Melia toosendan Sieb. Et Zucc could be explored as a potential antioxidant and therapeutics.
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310027, China.
authors.forename: Liang
authors.initials: L
authors.lastname: He
authors.forename: Pengfei
authors.initials: P
authors.lastname: Ji
authors.forename: Xingguo
authors.initials: X
authors.lastname: Gong
authors.forename: Weiqi
authors.initials: W
authors.lastname: Li
authors.forename: Junwen
authors.initials: J
authors.lastname: Cheng
authors.forename: Hua
authors.initials: H
authors.lastname: Qian
authors.forename: Xianliang
authors.initials: X
authors.lastname: Song
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D001713:Biphenyl Compounds / Q000737:chemistry; D045744:Cell Line, Tumor; D055598:Chemical Phenomena; D002850:Chromatography, Gel; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D005638:Fruit / Q000737:chemistry*; D006801:Humans; D017665:Hydroxyl Radical / Q000737:chemistry; D031585:Melia / Q000737:chemistry*; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis; D010851:Picrates / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D013481:Superoxides / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D009005:Monosaccharides; D010851:Picrates; D011134:Polysaccharides; D013481:Superoxides; D017665:Hydroxyl Radical; C004931:1,1-diphenyl-2-picrylhydrazyl