title: Osteogenic differentiation of GFP-labeled human umbilical cord blood derived mesenchymal stem cells after cryopreservation.
abstract: The osteogenic capacity of human umbilical cord blood derived mesenchymal stem cells (UCB-MSCs) has been demonstrated both in vitro and in vivo. Therefore, cell labeling and storage are becoming necessary for researching the potential therapeutic use of UCB-MSCs for bone tissue engineering. The aim of this study was to determine the effect of cryopreservation on the osteogenic differentiation of green fluorescent protein (GFP)-marked UCB-MSCs in vitro. MSCs were isolated from full-term human UCB, expanded, transfected with the GFP gene, and then cryopreserved in liquid nitrogen for 4 weeks. After thawing, cell surface antigen markers and osteogenic potential were analyzed, and the luminescence of these cells was observed by fluorescence microscopy. The results demonstrate that cryopreservation has no effect on the cell phenotype, GFP expression or osteogenic differentiation of UCB-MSCs, showing that cryopreserved GFP-labeled UCB-MSCs might be applied for bone tissue engineering.
authors.affiliation: Department of Plastic and Reconstructive Surgery, Shanghai 10th People's Hospital, Shanghai 200072, China.
authors.forename: Guangpeng
authors.initials: G
authors.lastname: Liu
authors.forename: Xinhai
authors.initials: X
authors.lastname: Ye
authors.forename: Yuchang
authors.initials: Y
authors.lastname: Zhu
authors.forename: Yulin
authors.initials: Y
authors.lastname: Li
authors.forename: Jian
authors.initials: J
authors.lastname: Sun
authors.forename: Lei
authors.initials: L
authors.lastname: Cui
authors.forename: Yilin
authors.initials: Y
authors.lastname: Cao
mesh_terms: D002454:Cell Differentiation; D002478:Cells, Cultured; D015925:Cryopreservation; D005312:Fetal Blood / Q000166:cytology*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008856:Microscopy, Fluorescence; D010012:Osteogenesis / Q000502:physiology*; D014162:Transfection
chemical_list: D049452:Green Fluorescent Proteins