title: Nested polymerase chain reaction-based detection of Dothistroma septosporum, red band needle blight of pine, a tool in support of phytosanitary regimes.
abstract: Red band needle blight is one of the most important foliar diseases of Pinus species and is of increasing international forest health and biosecurity concern. To provide a rapid identification technique for this pathogen in support of official control measures, a nested polymerase chain reaction-based diagnostic assay that employs species-specific primer sets has been developed. The assay is able to detect the presence of the pathogen direct from pine needles, irrespective of host species, to within 10 fg of target DNA, the equivalent of approximately 2-3 ascospores or hyphael cells.
authors.affiliation: Laboratoire de Pathologie Forestière, Unité de Recherches en Santé Végétale, Centre de Recherches de Bordeaux, Institute National de la Recherche Agronomique, BP81, 33883 Villenave d'Ornon Cedex, France. Stephen.langrell@ec.europa.eu
authors.forename: Stephen R H
authors.initials: SR
authors.lastname: Langrell
mesh_terms: D001203:Ascomycota / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D017931:DNA Primers / Q000235:genetics; D028223:Pinus / Q000382:microbiology*; D010935:Plant Diseases / Q000382:microbiology*; D018515:Plant Leaves / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers