title: Rhamnolipid from Pseudomonas desmolyticum NCIM-2112 and its role in the degradation of Brown 3REL.
abstract: The biosurfactant produced by Pseudomonas desmolyticum NCIM 2112 (Pd 2112) was confirmed as rhamnolipid based on the formation of dark blue halos around the colonies in CTAB-methylene blue agar plates and the content of rhamnose sugar. The average yield of rhamnolipid was 0.398 g/l/day when grown on hexadecane as sole carbon source. Pd 2112 emulsification potential associated with cell free culture broth was stable for 72 h using various hydrocarbons and vegetable oils. Chemical structure of the biosurfactant was identified as mono-rhamnolipid (Rha-C(6) -C(8) ) using HPTLC, fourier transform infrared spectroscopy, (1) H and (13) C NMR and gas chromatography-mass spectroscopy analysis. Pd 2112 mono-rhamnolipid (1 mg/ml) had increased permeabilization of Bacillus sp VUS NCIM 5342 and increased decolorization rate of textile dye Brown 3REL by 50%. Extracellular activities of lignin peroxidase and veratryl alcohol oxidase, enzymes involved in dye degradation, were significantly increased in the presence of mono-rhamnolipid by 324.52% and 100% respectively. Scanning electron micro-scopy observations revealed that rhamnolipid did not exert any disruptive action on Bacillus cells as compared to Tween 80. The mono-rhamnolipid of Pd 2112 has potential for its application in biodegradation of textile dyes.
authors.affiliation: Department of Microbiology, Shivaji University, Kolhapur, India.
authors.forename: Mital
authors.initials: M
authors.lastname: Jadhav
authors.forename: Satish
authors.initials: S
authors.lastname: Kalme
authors.forename: Dhawal
authors.initials: D
authors.lastname: Tamboli
authors.forename: Sanjay
authors.initials: S
authors.lastname: Govindwar
mesh_terms: D000429:Alcohol Oxidoreductases / Q000378:metabolism; D001407:Bacillus / Q000201:enzymology* / Q000648:ultrastructure; D001673:Biodegradation, Environmental; D004396:Coloring Agents / Q000737:chemistry / Q000378:metabolism*; D003651:Decanoates / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D045703:Emulsifying Agents / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D004785:Environmental Pollutants / Q000378:metabolism*; D006017:Glycolipids / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D007220:Industrial Waste; D010544:Peroxidases / Q000378:metabolism*; D011549:Pseudomonas / Q000378:metabolism*; D012210:Rhamnose / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D013783:Textile Industry; D013997:Time Factors
chemical_list: D004396:Coloring Agents; D003651:Decanoates; D045703:Emulsifying Agents; D004785:Environmental Pollutants; D006017:Glycolipids; D007220:Industrial Waste; C418382:rhamnolipid; C085734:rhamnopyranosyl-3-hydroxydecanoyl-3-hydroxydecanoate; D000429:Alcohol Oxidoreductases; C057990:veratryl alcohol oxidase; D010544:Peroxidases; C042858:lignin peroxidase; D012210:Rhamnose