title: A new nitrosyl ruthenium complex: synthesis, chemical characterization, in vitro and in vivo antitumor activities and probable mechanism of action.
abstract: This study describes the synthesis of a new ruthenium nitrosyl complex with the formula [RuCl(2)NO(BPA)] [BPA = (2-hydroxybenzyl)(2-methylpyridyl)amine ion], which was synthesized and characterized by spectroscopy, cyclic voltammetry, X-ray crystallography, and theoretical calculation data. The biological studies of this complex included in vitro cytotoxic assays, which revealed its activity against two different tumor cell lines (HeLa and Tm5), with efficacy comparable to that of cisplatin, a metal-based drug that is administered in clinical treatment. The in vivo studies showed that [RuCl(2)NO(BPA)]is effective in reducing tumor mass. Also, our results suggest that the mechanism of action of [RuCl(2)NO(BPA)] includes binding to DNA, causing fragmentation of this biological molecule, which leads to apoptosis.
authors.affiliation: Departamento de Bioquímica e Imunologia, Faculdade de Medicine de Ribeirão Preto, Universidade de São Paulo, Av Bandeirantes, 3900-FMRP-USP, 14.049-900 Ribeirão Preto, SP, Brazil.
authors.forename: Tassiele A
authors.initials: TA
authors.lastname: Heinrich
authors.forename: Gustavo
authors.initials: G
authors.lastname: Von Poelhsitz
authors.forename: Rosana I
authors.initials: RI
authors.lastname: Reis
authors.forename: Eduardo E
authors.initials: EE
authors.lastname: Castellano
authors.forename: Ademir
authors.initials: A
authors.lastname: Neves
authors.forename: Maurício
authors.initials: M
authors.lastname: Lanznaster
authors.forename: Sérgio P
authors.initials: SP
authors.lastname: Machado
authors.forename: Alzir A
authors.initials: AA
authors.lastname: Batista
authors.forename: Claudio M
authors.initials: CM
authors.lastname: Costa-Neto
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology*; D016466:CHO Cells; D045744:Cell Line, Tumor; D006224:Cricetinae; D003412:Cricetulus; D018360:Crystallography, X-Ray; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D008958:Models, Molecular; D017975:Ruthenium Compounds / Q000737:chemistry* / Q000494:pharmacology*; D013057:Spectrum Analysis / Q000379:methods
chemical_list: D000970:Antineoplastic Agents; D017975:Ruthenium Compounds