title: Convenient synthesis of nucleoside 5'-triphosphates for RNA transcription.
abstract: By generating a selective phosphitylating reagent in situ, nucleoside 5'-triphosphates can be conveniently synthesized in one pot. This novel strategy without nucleoside protection has been developed to largely simplify synthesis of the nucleoside triphosphates. This demonstrated principle can be applied to the 5'-triphosphate synthesis of both native and modified nucleosides.
authors.affiliation: Department of Chemistry, Georgia State University, Atlanta, Georgia, USA.
authors.forename: Julianne
authors.initials: J
authors.lastname: Caton-Williams
authors.forename: Lina
authors.initials: L
authors.lastname: Lin
authors.forename: Matthew
authors.initials: M
authors.lastname: Smith
authors.forename: Zhen
authors.initials: Z
authors.lastname: Huang
mesh_terms: D001483:Base Sequence; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D009711:Nucleotides / Q000138:chemical synthesis* / Q000737:chemistry* / Q000378:metabolism; D011122:Polyphosphates / Q000737:chemistry*; D012313:RNA / Q000096:biosynthesis / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D009711:Nucleotides; D011122:Polyphosphates; D012313:RNA; D012321:DNA-Directed RNA Polymerases; C005692:triphosphoric acid