title: Single nucleotide polymorphism discovery in elite North American potato germplasm.
abstract: BACKGROUND
  Current breeding approaches in potato rely almost entirely on phenotypic evaluations; molecular markers, with the exception of a few linked to disease resistance traits, are not widely used. Large-scale sequence datasets generated primarily through Sanger Expressed Sequence Tag projects are available from a limited number of potato cultivars and access to next generation sequencing technologies permits rapid generation of sequence data for additional cultivars. When coupled with the advent of high throughput genotyping methods, an opportunity now exists for potato breeders to incorporate considerably more genotypic data into their decision-making.
  
  
  RESULTS
  To identify a large number of Single Nucleotide Polymorphisms (SNPs) in elite potato germplasm, we sequenced normalized cDNA prepared from three commercial potato cultivars: 'Atlantic', 'Premier Russet' and 'Snowden'. For each cultivar, we generated 2 Gb of sequence which was assembled into a representative transcriptome of ~28-29 Mb for each cultivar. Using the Maq SNP filter that filters read depth, density, and quality, 575,340 SNPs were identified within these three cultivars. In parallel, 2,358 SNPs were identified within existing Sanger sequences for three additional cultivars, 'Bintje', 'Kennebec', and 'Shepody'. Using a stringent set of filters in conjunction with the potato reference genome, we identified 69,011 high confidence SNPs from these six cultivars for use in genotyping with the Infinium platform. Ninety-six of these SNPs were used with a BeadXpress assay to assess allelic diversity in a germplasm panel of 248 lines; 82 of the SNPs proved sufficiently informative for subsequent analyses. Within diverse North American germplasm, the chip processing market class was most distinct, clearly separated from all other market classes. The round white and russet market classes both include fresh market and processing cultivars. Nevertheless, the russet and round white market classes are more distant from each other than processing are from fresh market types within these two groups.
  
  
  CONCLUSIONS
  The genotype data generated in this study, albeit limited in number, has revealed distinct relationships among the market classes of potato. The SNPs identified in this study will enable high-throughput genotyping of germplasm and populations, which in turn will enable more efficient marker-assisted breeding efforts in potato.
authors.affiliation: Department of Plant Biology, Michigan State University, East Lansing, MI 48824, USA.
authors.forename: John P
authors.initials: JP
authors.lastname: Hamilton
authors.forename: Candice N
authors.initials: CN
authors.lastname: Hansey
authors.forename: Brett R
authors.initials: BR
authors.lastname: Whitty
authors.forename: Kevin
authors.initials: K
authors.lastname: Stoffel
authors.forename: Alicia N
authors.initials: AN
authors.lastname: Massa
authors.forename: Allen
authors.initials: A
authors.lastname: Van Deynze
authors.forename: Walter S
authors.initials: WS
authors.lastname: De Jong
authors.forename: David S
authors.initials: DS
authors.lastname: Douches
authors.forename: C Robin
authors.initials: CR
authors.lastname: Buell
mesh_terms: D000483:Alleles; D019976:Cloning, Organism; D020224:Expressed Sequence Tags / Q000378:metabolism; D020869:Gene Expression Profiling; D023281:Genomics; D005838:Genotype; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D017422:Sequence Analysis, DNA; D011198:Solanum tuberosum / Q000235:genetics*