title: Electrospinning of polyvinyl alcohol/gelatin nanofiber composites and cross-linking for bone tissue engineering application.
abstract: A three-dimensional polymer composite system consisting of polyvinyl alcohol/gelatin (PVA/GE) was fabricated via the electrospinning method and physically cross linked by methanol treatment. The effects of cross-linking between PVA/GE blend on physical, mechanical, and biological properties were investigated. After treating with methanol, PVA/GE mats become dense, hard, and aggregative with increased resistance to water dissolution. Osteoblasts like MG-63 cells were seeded on the surfaces of the cross linked PVA/GE mats and were found to attach firmly by expressing philopodial extensions. In addition, MTT assay and Western Blot analysis confirmed that the cells readily proliferated on the cross linked PVA/GE scaffolds. The osteoblast cell-matrix interaction demonstrated that the active biocompatibility of the mats was facilitated by using GE and cross-linking. In conclusion, our results suggest that cross-linked PVA/GE scaffolds hold promise for tissue engineering applications, especially in the field of artificial bone implant.
authors.affiliation: Department of Biomedical Engineering and Materials, College of Medicine, Soonchunhyang University, 366-1 Ssangyong dong, Cheonan 330-090, Korea.
authors.forename: Nguyen Thuy Ba
authors.initials: NT
authors.lastname: Linh
authors.forename: Byong-Taek
authors.initials: BT
authors.lastname: Lee
mesh_terms: D001842:Bone and Bones / Q000737:chemistry*; D002152:Calorimetry, Differential Scanning; D002460:Cell Line; D005780:Gelatin / Q000737:chemistry*; D006801:Humans; D057139:Nanofibers; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D023822:Tissue Engineering
chemical_list: D005780:Gelatin; D011142:Polyvinyl Alcohol