title: Genotype diversity of group A rotavirus strains in children with acute diarrhea in urban Burkina Faso, 2008-2010.
abstract: In this study, the diversity of G and P genotypes of rotavirus strains in Burkinabe children were examined. Between November 2008 and February 2010, 447 stool samples were collected from children <5 years of age with acute diarrhea visiting hospital in Ouagadougou. Group A rotavirus was previously detected in 151/447 (33.8%) of the samples tested by an immunochromatographic test and these samples were now tested further for rotavirus G and P genotypes by RT-PCR. Of these, the rotavirus type genes were amplified by RT-PCR for 140/151 (92.7%) samples and G and P genotypes were successfully determined for 81 (57.9%) and 130 (92.9%) samples, respectively. The most prevalent G genotypes were G1, 34/140 (24.3%), and G9, 21/140 (15%), while the predominant P genotypes were P[6], 56/140 (40%), and P[8], 54/140 (38.6%). Among the single infections, 63/140 (45%), the predominant G/P combinations were: G1P[8] (33%), G9P[8] (29%), and G2P[6] (14%). The unusual strains G1P[9] (3%), G12P[6] (3%), G10P[6] (2%), and G2P[8] (2%) were also detected. In a high number of strains 61/140 (43.6%), the G genotype could not be determined and mixed infections were determined in 17/140 (12.1%) of strains identified. This study highlights the high diversity and presence of unusual rotavirus strains in children in Burkina Faso.
authors.affiliation: Université de Ouagadougou, Burkina Faso. ouindgueta@gmail.com
authors.forename: Isidore J O
authors.initials: IJ
authors.lastname: Bonkoungou
authors.forename: Susan
authors.initials: S
authors.lastname: Damanka
authors.forename: Idrissa
authors.initials: I
authors.lastname: Sanou
authors.forename: Fidèle
authors.initials: F
authors.lastname: Tiendrébéogo
authors.forename: Sheick O
authors.initials: SO
authors.lastname: Coulibaly
authors.forename: Fabienne
authors.initials: F
authors.lastname: Bon
authors.forename: Kaisa
authors.initials: K
authors.lastname: Haukka
authors.forename: Alfred S
authors.initials: AS
authors.lastname: Traoré
authors.forename: Nicolas
authors.initials: N
authors.lastname: Barro
authors.forename: George E
authors.initials: GE
authors.lastname: Armah
mesh_terms: D002050:Burkina Faso / Q000453:epidemiology; D002675:Child, Preschool; D003967:Diarrhea / Q000453:epidemiology* / Q000821:virology*; D005243:Feces / Q000821:virology; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D015995:Prevalence; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000453:epidemiology* / Q000821:virology*
chemical_list: D012367:RNA, Viral