title: Detection of single nucleotide polymorphisms by a gold nanowire-on-film SERS sensor coupled with S1 nuclease treatment.
abstract: Single nucleotide polymorphisms (SNPs) can serve as important biomarkers for genetic diseases, for which accurate detection of SNPs is essential for early diagnosis. We have developed a novel SNP sensor by combining a Au nanowire-on-film surface-enhanced Raman scattering (SERS) platform with S1 nuclease reaction. The combined sensor system provides reproducible SERS signals only in the presence of perfectly matched target DNAs, to probe DNAs as a result of single-stranded DNA-specific degradation by S1 nuclease. Furthermore, point mutations in DNA causing Wilson disease and Avellino corneal dystrophy were successfully identified by this sensor, thereby indicating its practical ability to diagnose genetic diseases.
authors.affiliation: Department of Chemical and Biomolecular, Engineering (BK21 Program), KAIST, Daejeon 305-701, Korea.
authors.forename: Seung Min
authors.initials: SM
authors.lastname: Yoo
authors.forename: Taejoon
authors.initials: T
authors.lastname: Kang
authors.forename: Bongsoo
authors.initials: B
authors.lastname: Kim
authors.forename: Sang Yup
authors.initials: SY
authors.lastname: Lee
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D004720:Endonucleases / Q000378:metabolism*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D053768:Metal Nanoparticles; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D013059:Spectrum Analysis, Raman / Q000379:methods
chemical_list: D006046:Gold; D004247:DNA; D004720:Endonucleases