title: Protective effects of N-acetyl cysteine on lipid peroxide metabolism on isoproterenol-induced myocardial infarcted rats.
abstract: The present study was designed to evaluate the preventive effects of N-acetyl cysteine on lipid peroxide metabolism in isoproterenol (ISO) induced myocardial infarcted rats. Male albino Wistar rats were pretreated with N-acetyl cysteine (5 and 10 mg/kg) daily for a period of 14 days. After the pretreatment period, ISO (100 mg/kg) was subcutaneously injected to rats twice at an interval of 24 h. Increased activities of serum creatine kinase, creatine kinase-MB, lactate dehydrogenase, and increased intensities of serum lactate dehydrogenase-isoenzyme bands (LDH-1, LDH-2) were observed in ISO-induced rats. The heart lipid peroxidation products were significantly increased, and the antioxidant system was significantly reduced in ISO-induced rats. Pretreatment with N-acetyl cysteine (5 and 10 mg/kg) to ISO-induced rats showed significant effects on all the biochemical parameters studied. Histopathological findings of the myocardium also showed the protective role of N-acetyl cysteine in ISO-induced rats. Furthermore, in vitro study confirmed the potent-free radical scavenging activity of N-acetyl cysteine. The effect at a dose of 10 mg/kg of N-acetyl cysteine was more pronounced than the dose, 5 mg/kg. The results of our study show that N-acetyl cysteine protects the heart against ISO-induced myocardial infarction by its free radical scavenging effect.
authors.affiliation: Department of Biochemistry and Biotechnology, Annamalai University, Tamil Nadu, India.
authors.forename: Mohamed Fizur
authors.initials: MF
authors.lastname: Nagoor Meeran
authors.forename: Ponnian Stanely
authors.initials: PS
authors.lastname: Mainzen Prince
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology*; D000818:Animals; D001205:Ascorbic Acid / Q000097:blood; D001713:Biphenyl Compounds / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D052279:Creatine Kinase, MB Form / Q000097:blood; D016166:Free Radical Scavengers / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D007527:Isoenzymes / Q000378:metabolism; D007545:Isoproterenol / Q000633:toxicity*; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008054:Lipid Peroxides / Q000378:metabolism*; D008297:Male; D009203:Myocardial Infarction / Q000097:blood / Q000139:chemically induced* / Q000201:enzymology / Q000378:metabolism*; D009206:Myocardium / Q000201:enzymology / Q000473:pathology; D010851:Picrates / Q000378:metabolism; D020011:Protective Agents / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014810:Vitamin E / Q000097:blood
chemical_list: D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D007527:Isoenzymes; D008054:Lipid Peroxides; D010851:Picrates; D020011:Protective Agents; D017392:Thiobarbituric Acid Reactive Substances; D014810:Vitamin E; C004931:1,1-diphenyl-2-picrylhydrazyl; D007770:L-Lactate Dehydrogenase; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D052279:Creatine Kinase, MB Form; D005978:Glutathione; D007545:Isoproterenol; D001205:Ascorbic Acid; D000111:Acetylcysteine