title: The viability to a wall shear stress and propagation of Bifidobacterium longum in the intensive membrane bioreactor.
abstract: Bifidobacterium longum grew at 65 L pilot scale of the membrane bioreactor (MBR), externally fitted with ceramic membrane (0.7 m2). Cell mass at the MBR reached 22.18 g L(-1) as dry cell weight in 12 h, which is 8.44 times higher than cell mass attained at the vial culture. The growth rate in the vial culture was μ = 0.385 h- and at the batch culture was μ = 1.13 h- in the exponential period and μ = 0.31 h(-1) in the stationary period. In the fed-batch mode was μ = 1.102 h(-1) for 6 h with inoculation and declined to μ = 0.456 h(-1) with feeding of feed medium. The growth rate at the MBR was μ = 0.134 h(-1). The number of viable cells was 6.01 × 10(12) cfu L(-1) at the batch culture, but increased to 1.15 × 10(14) cfu L(-1) at the MBR culture. The specific growth rate of viable cell number (colony-forming units per liter, per hour) improved by 6.01 times from the batch to the MBR culture. The wall shear stress mainly generated by the pump, and the membrane incorporated into the MBR was controlled during the cultivation at the MBR. The viability of B. longum declined to under 10% in the first 2 weeks of the 4-week stability test (40° C) as B. longum was exposed to over wall shear stress 713 Pa, but the viability improved to 30-40% in wall shear stress of 260 Pa or STR culture. The loss in the cell viability can be saved by managing with wall shear stress during the cultivation at the MBR.
authors.affiliation: Department of Biotechnology, Pukyong National University, Daeyun 3-dong, Busan, Republic of Korea. ilseon5521@yahoo.co.kr
authors.forename: Ilseon S
authors.initials: IS
authors.lastname: Jung
authors.forename: Min Kyo
authors.initials: MK
authors.lastname: Oh
authors.forename: Young Chae
authors.initials: YC
authors.lastname: Cho
authors.forename: In Soo
authors.initials: IS
authors.lastname: Kong
mesh_terms: D061249:Batch Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D001644:Bifidobacterium / Q000737:chemistry / Q000254:growth & development*; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology*; D005285:Fermentation; D007700:Kinetics; D008567:Membranes, Artificial; D050296:Microbial Viability; D013314:Stress, Mechanical
chemical_list: D008567:Membranes, Artificial