title: Antioxidant and antimelanogenic behaviors of Paeonia suffruticosa.
abstract: Antioxidant properties of eight Paeonia suffruticosa (Ps) extracts (Ps-1 to Ps-8) were evaluated. The respective half maximally effective concentration (EC(50)) values of Ps-1 ~ 8 were 10.0, 9.8, 63.6, >100, 3.8, 85.1, 6.9, and 0.7 μg/ml for 1,1-diphenyl-2-picrylhydrazyl radical (DPPH·) radical scavenging efficiency and 22.9, 11.4, 53.1, >100, 7.5, 97.6, 43.7, 4.2 μg/ml for 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS·(+)) radical scavenging capacity. The Ps-8 exhibited high free radical scavenging capacity, ion-chelating ability, reducing power, and inhibition of lipid peroxidation, which may have been attributable to its abundant phenolic and flavonoid content. In Hs68 and B16 cells treated with 100 μg/ml Ps-1, Ps-3, Ps-4 and Ps-6, expressions of toxic activities were lower than those in cells treated with arbutin and ascorbic acid. The antimelanogenesis properties were also tested in B16 cells. Extract Ps-1, and particularly extract Ps-6, considerably inhibited cellular tyrosinase and 3,4-dihydroxyphenylalanine (DOPA) oxidase activity and also reduced melanin content in B16 cells by down-expression of melanocortin-1 receptor (MC1R), microphthalmia-associated transcription factor (MITF), tyrosinase, and tyrosinase-related proteins-1 (TRP-1). The results suggest that P. suffruticosa extracts have antioxidant and antimelanogenesis activities with potential applications in cosmetic materials or food additives.
authors.affiliation: Institute of Cosmetic Science, Chia Nan University of Pharmacy and Science, Tainan, Taiwan.
authors.forename: Hsiou-Yu
authors.initials: HY
authors.lastname: Ding
authors.forename: Tzung-Han
authors.initials: TH
authors.lastname: Chou
authors.forename: Rong-Jyh
authors.initials: RJ
authors.lastname: Lin
authors.forename: Leong-Perng
authors.initials: LP
authors.lastname: Chan
authors.forename: Guey-Horng
authors.initials: GH
authors.lastname: Wang
authors.forename: Chia-Hua
authors.initials: CH
authors.lastname: Liang
mesh_terms: D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D002614:Chelating Agents / Q000494:pharmacology; D004295:Dihydroxyphenylalanine / Q000378:metabolism; D015536:Down-Regulation; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D007370:Interferon Type I / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008543:Melanins / Q000378:metabolism*; D008546:Melanoma, Experimental; D051739:Microphthalmia-Associated Transcription Factor / Q000378:metabolism; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors; D029596:Paeonia / Q000737:chemistry*; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology*; D008517:Phytotherapy; D024301:Plant Bark; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D018517:Plant Roots; D011257:Pregnancy Proteins / Q000378:metabolism; D044102:Receptor, Melanocortin, Type 1 / Q000378:metabolism
chemical_list: D000975:Antioxidants; D002614:Chelating Agents; D005419:Flavonoids; D007370:Interferon Type I; D008543:Melanins; D051739:Microphthalmia-Associated Transcription Factor; D010636:Phenols; D010936:Plant Extracts; D011257:Pregnancy Proteins; D044102:Receptor, Melanocortin, Type 1; C020947:interferon tau; D004295:Dihydroxyphenylalanine; D014442:Monophenol Monooxygenase