title: Changes in the metabolic profile of rat liver after α-tocopherol deficiency as revealed by metabolomics analysis.
abstract: Metabolomics is an approach in which the profiles of metabolites in different tissues and/or biofluids are investigated to understand the changes induced following a modulation. We used this approach to investigate the biochemical effects of α-tocopherol in the liver using a rat model. Rats (21-day-old) were fed either an α-tocopherol-sufficient control (n = 10) or an α-tocopherol-deficient (n = 10) diet for 2 months before sacrifice. Livers were homogenized in methanol-chloroform-water (3 : 1 : 1, v/v/v), and the polar phase extracts of the liver samples were analyzed using (1) H NMR. Multivariate statistical analysis of the data was performed using principal component analysis and orthogonal partial least squares-discriminant analysis. Identification of (1) H NMR signals was performed primarily using the Human Metabolome Database, Biological Magnetic Resonance Data Bank and previous literature, and confirmed by spiking with metabolites and applying two-dimensional NMR. The statistical analysis revealed that α-tocopherol deficiency caused an increase in carnitine, choline, L-valine, L-lysine, tyrosine and inosine content and a reduction in glucose and uridine 5'-monophosphate content. Changes in carnitine and glucose suggest a possible shift in energy metabolism.
authors.affiliation: Department of Food Science, Swedish University of Agricultural Sciences, Uppsala, Sweden. Ali.Moazzami@lmv.slu.se
authors.forename: Ali A
authors.initials: AA
authors.lastname: Moazzami
authors.forename: Roger
authors.initials: R
authors.lastname: Andersson
authors.forename: Afaf
authors.initials: A
authors.lastname: Kamal-Eldin
mesh_terms: D000818:Animals; D006801:Humans; D016018:Least-Squares Analysis; D008099:Liver / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D055442:Metabolome; D055432:Metabolomics / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014020:Tissue Extracts / Q000378:metabolism; D024502:alpha-Tocopherol / Q000097:blood / Q000378:metabolism*
chemical_list: D014020:Tissue Extracts; D024502:alpha-Tocopherol