title: Pulpal progenitors and dentin repair.
abstract: Mesenchymal stem cells are present in the dental pulp. They have been shown to contribute to dentin-like tissue formation in vitro and to participate in bone repair after a mandibular lesion. However, their capacity to contribute efficiently to reparative dentin formation after pulp lesion has never been explored. After pulp exposure, we have identified proliferative cells within 3 zones. In the crown, zone I is near the cavity, and zone II corresponds to the isthmus between the mesial and central pulp. In the root, zone III, near the apex, at a distance from the inflammatory site, contains mitotic stromal cells which may represent a source of progenitor cells. Stem-cell-based strategies are promising treatments for tissue injury in dentistry. Our experiments focused on (1) location of stem cells induced to leave their quiescent state early after pulp injury and (2) implantation of pulp progenitors, a substitute for classic endodontic treatments, paving the way for pulp stem-cell-based therapies.
authors.affiliation: INSERM UMR-S, UFR Biomédicales des Saints-Pères, Université Paris Descartes, France.
authors.forename: Y
authors.initials: Y
authors.lastname: Harichane
authors.forename: A
authors.initials: A
authors.lastname: Hirata
authors.forename: S
authors.initials: S
authors.lastname: Dimitrova-Nakov
authors.forename: I
authors.initials: I
authors.lastname: Granja
authors.forename: A
authors.initials: A
authors.lastname: Goldberg
authors.forename: O
authors.initials: O
authors.lastname: Kellermann
authors.forename: A
authors.initials: A
authors.lastname: Poliard
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D003782:Dental Pulp / Q000166:cytology*; D003786:Dental Pulp Cavity / Q000166:cytology; D003788:Dental Pulp Diseases / Q000628:therapy; D003789:Dental Pulp Exposure / Q000473:pathology; D003809:Dentin, Secondary / Q000502:physiology*; D003810:Dentinogenesis / Q000502:physiology; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D008938:Mitosis; D010012:Osteogenesis / Q000502:physiology; D019227:Tooth Apex / Q000166:cytology; D019228:Tooth Crown / Q000166:cytology; D014945:Wound Healing / Q000502:physiology