title: SUMO1 attenuates stress-induced ROS generation by inhibiting NADPH oxidase 2.
abstract: Small ubiquitin-like modifier 1 (SUMO1) is a member of the superfamily of ubiquitin-like proteins. Despite its structural similarity with ubiquitin, SUMO1 does not seem to play any role in protein degradation and its precise biological function is poorly understood. During our studies on heat-shock responses, we found that heat-shock stress increased SUMO1 conjugation in a dose-dependent manner. Intriguingly, SUMO1 conjugation resulted in decrease of intracellular ROS generation and protection cells from death under heat-shock stress. We showed that NADPH oxidase 2 (NOX2) is a target protein of sumoylation by SUMO1 using immunoprecipitation and is colocalized with SUMO1 at plasma membrane. Additionally, we demonstrated that the attenuation in intracellular ROS generation resulted from inhibition of NADPH oxidase complex (NOX) activity. These results suggested that SUMO1 plays an important role in modulation of NOX activity required for ROS generation.
authors.affiliation: The Center for Cell Signaling & Drug Discovery Research, College of Pharmacy, Division of Life and Pharmaceutical Sciences, Department of Bioinspired Science, Ewha Womans University, Seoul 120-750, South Korea.
authors.forename: Hyun Jung
authors.initials: HJ
authors.lastname: Kim
authors.forename: Jiwon
authors.initials: J
authors.lastname: Yun
authors.forename: Jiyoung
authors.initials: J
authors.lastname: Lee
authors.forename: Hyunkyung
authors.initials: H
authors.lastname: Hong
authors.forename: Jaeho
authors.initials: J
authors.lastname: Jeong
authors.forename: Eunhee
authors.initials: E
authors.lastname: Kim
authors.forename: Yun Soo
authors.initials: YS
authors.lastname: Bae
authors.forename: Kong-Joo
authors.initials: KJ
authors.lastname: Lee
mesh_terms: D017209:Apoptosis; D002462:Cell Membrane / Q000378:metabolism; D004789:Enzyme Activation; D057809:HEK293 Cells; D006367:HeLa Cells; D018869:Heat-Shock Response; D006801:Humans; D048031:JNK Mitogen-Activated Protein Kinases / Q000096:biosynthesis; D008562:Membrane Glycoproteins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D025842:SUMO-1 Protein / Q000235:genetics / Q000378:metabolism*; D058207:Sumoylation; D048051:p38 Mitogen-Activated Protein Kinases / Q000096:biosynthesis
chemical_list: D008562:Membrane Glycoproteins; D017382:Reactive Oxygen Species; D025842:SUMO-1 Protein; C495501:SUMO1 protein, human; C056558:CYBB protein, human; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases; D048031:JNK Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases