title: Extraction of polysaccharides and the antioxidant activity from the seeds of Plantago asiatica L.
abstract: The extraction conditions of polysaccharides from Plantago asiatica L. seeds were investigated. Four parameters affecting the polysaccharides extraction, extraction times, water to sample, extraction temperature and single extraction time, were determined by orthogonal experiments. Under the optimized conditions, the polysaccharides yield of P. asiatica L. seeds was 2.467%. The antioxidant activities of the polysaccharides were investigated. The reducing power of the polysaccharides was dose dependent, and the reducing capacity of the polysaccharides was inferior to butylated hydroxytoluene, which is known to be a strong reducing agent. The scavenging rates of the polysaccharides on superoxide and 1,1-diphenyl-2-picrylhydrazyl radicals were 79.7% and 81.4%, at polysaccharides concentration of 0.75 mg/mL, respectively, a scavenging rates approximately similar to that of 0.75 mg/mL ascorbic acid (83.5% and 85.1%, respectively). Furthermore, it exhibited a moderate concentration-dependent ABTS radical scavenging activity, ferrous ion chelating potency and H(2)O(2) scavenging activity. The data obtained in the in vitro models clearly establish the antioxidant potency of the polysaccharides extracted from Semen Plantaginis.
authors.affiliation: School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou, PR China. chlye2005@126.com
authors.forename: Chun-Lin
authors.initials: CL
authors.lastname: Ye
authors.forename: Wei-Lian
authors.initials: WL
authors.lastname: Hu
authors.forename: De-Hui
authors.initials: DH
authors.lastname: Dai
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology; D052160:Benzothiazoles / Q000378:metabolism; D001713:Biphenyl Compounds / Q000378:metabolism; D002084:Butylated Hydroxytoluene / Q000378:metabolism; D002614:Chelating Agents / Q000494:pharmacology; D005591:Chemical Fractionation; D004492:Edetic Acid / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D010851:Picrates / Q000378:metabolism; D010943:Plantago / Q000737:chemistry*; D011134:Polysaccharides / Q000302:isolation & purification* / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry*; D013451:Sulfonic Acids / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism
chemical_list: D000975:Antioxidants; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D002614:Chelating Agents; D016166:Free Radical Scavengers; D010851:Picrates; D011134:Polysaccharides; D013451:Sulfonic Acids; D013481:Superoxides; D002084:Butylated Hydroxytoluene; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D004492:Edetic Acid; D006861:Hydrogen Peroxide; C004931:1,1-diphenyl-2-picrylhydrazyl; D001205:Ascorbic Acid