title: Association between single-nucleotide polymorphisms in pre-miRNAs and the risk of asthma in a Chinese population.
abstract: Single-nucleotide polymorphisms (SNPs) in pre-miRNAs may alter microRNA (miRNA) expression levels or processing and contribute to susceptibility to a wide range of diseases. We investigated the correlation between four SNPs (rs11614913, rs3746444, rs2910164, and rs229283) in pre-miRNAs and the risk of asthma in 220 asthma patients and 540 controls using polymerase chain reaction-restriction fragment length polymorphism methodology and DNA-sequencing. There were significant differences in the genotype and allelic distribution of rs2910164G/C and rs2292832C/T polymorphisms among cases and controls. The CC genotype and C allele of rs2910164G/C were significantly associated with a decreased risk of asthma (CC vs. GG, odds ratio [OR] = 0.51, 95% confidence interval [CI]: 0.31-0.82; C vs. G, OR = 0.74, 95% CI: 0.59-0.93). Similarly, the TT genotype and T allele of rs2292832C/T were significantly associated with a decreased risk of asthma (TT vs. CC, OR = 0.56, 95% CI: 0.33-0.95; T vs. C, OR = 0.71, 95% CI: 0.53-0.95). However, no significant association between the other two polymorphisms (i.e., rs11614913C/T and rs3746444C/T) and the risk of asthma was observed. Our data indicate that rs2910164G/C and rs2292832C/T may play a role in the development of asthma.
authors.affiliation: Department of Forensic Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, Sichuan, China.
authors.forename: Xiao-Wei
authors.initials: XW
authors.lastname: Su
authors.forename: Yan
authors.initials: Y
authors.lastname: Yang
authors.forename: Mei-Li
authors.initials: ML
authors.lastname: Lv
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Dong
authors.lastname: Miao-Liao
authors.forename: Lin-Bo
authors.initials: LB
authors.lastname: Gao
authors.forename: Hai-Bo
authors.initials: HB
authors.lastname: Luo
authors.lastname: Yun-Liu
authors.forename: Rui-Juan
authors.initials: RJ
authors.lastname: Cong
authors.forename: Wei-Bo
authors.initials: WB
authors.lastname: Liang
authors.forename: Ying-Bi
authors.initials: YB
authors.lastname: Li
mesh_terms: D000328:Adult; D044466:Asian People / Q000235:genetics*; D001249:Asthma / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
chemical_list: D035683:MicroRNAs