title: Molecular cloning and expression analysis of CmMlo1 in melon.
abstract: Mlo gene encodes an important transmembrane protein that is involved in biotic/abiotic stresses. Using the method of homologous, we cloned a Mlo gene from melon, named CmMlo1. The gene is 1551 bp in length, encoding 516 amino acids; it has seven-transmembrane domain topology and is a typical transmembrane protein. Localization analysis in onion epidermal cells showed that CmMlo1-GFP is localized to the plasma membrane. RT-PCR results indicated that CmMlo1 is mainly expressed in melon cotyledon and flower, with a tissue-specific distribution manner. CmMlo1 expression is not obvious under powdery mildew stress, but under cadmium stress, its expression was significantly up-regulated, indicating that CmMlo1 is possibly involved in abiotic stress.
authors.affiliation: Institute of Vegetable, Gansu Academy of Agricultural Science, Lanzhou 730070, China. ch06038@sohu.com
authors.forename: Hong
authors.initials: H
authors.lastname: Cheng
authors.forename: Weiping
authors.initials: W
authors.lastname: Kun
authors.forename: Dongshun
authors.initials: D
authors.lastname: Liu
authors.forename: Yongquan
authors.initials: Y
authors.lastname: Su
authors.forename: Qiwei
authors.initials: Q
authors.lastname: He
mesh_terms: D001483:Base Sequence; D002104:Cadmium / Q000633:toxicity*; D003001:Cloning, Molecular; D028463:Cucumis melo / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000096:biosynthesis; D035264:Flowers / Q000378:metabolism; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D005822:Genetic Vectors / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013312:Stress, Physiological / Q000235:genetics*
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D008565:Membrane Proteins; D002104:Cadmium