title: Studies on the hemocompatibility of bacterial cellulose.
abstract: Among the strategies to improve a material's hemocompatibility, pre-coating with the tripeptide Arg-Gly-Asp (RGD) is used to favor endothelialization thus lowering thrombogenicity. The blood compatibility of native and RGD-modified bacterial cellulose (BC) was studied in this work for the first time. The plasma recalcification time and whole blood clotting results demonstrate the hemocompatibility of BC. A significant amount of plasma protein adsorb to BC fibres, however, according to analysis by intrinsic tryptophan fluorescence techniques when albumin, γ-globulin, and fibrinogen from pure protein solutions adsorb to BC do not undergo detectable conformational modifications. Human microvascular endothelial cells cultured on RGD-modified BC readily form a confluent cell layer, inhibiting the adhesion of platelets. As a general conclusion, both native and RGD-modified BCs may be classified as hemocompatible materials.
authors.affiliation: IBB-Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Campus of Gualtar, Braga 4710-057, Portugal.
authors.forename: Fábia K
authors.initials: FK
authors.lastname: Andrade
authors.forename: João Pedro
authors.initials: JP
authors.lastname: Silva
authors.forename: Manuela
authors.initials: M
authors.lastname: Carvalho
authors.forename: Elisabete M S
authors.initials: EM
authors.lastname: Castanheira
authors.forename: Raquel
authors.initials: R
authors.lastname: Soares
authors.forename: Miguel
authors.initials: M
authors.lastname: Gama
mesh_terms: D000327:Adsorption; D001419:Bacteria / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry* / Q000378:metabolism; D001777:Blood Coagulation; D001792:Blood Platelets / Q000378:metabolism / Q000648:ultrastructure; D001798:Blood Proteins / Q000378:metabolism; D002482:Cellulose / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D009842:Oligopeptides / Q000737:chemistry; D010973:Platelet Adhesiveness; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: D001672:Biocompatible Materials; D001798:Blood Proteins; D009842:Oligopeptides; D011994:Recombinant Proteins; C047981:arginyl-glycyl-aspartic acid; D002482:Cellulose