title: Benzonidazole levels in blood vary with age in rats.
abstract: Benznidazole (Bz) exhibits toxic side effects in animal studies and clinical use. Reductive metabolism of Bz in liver microsomes modulates the duration of its chemotherapeutic effect and its toxicity. The rate of this metabolism depends on age and is less intense in newborns and youngsters than in adults. In the present study, we determined Bz blood levels in rats of different ages that received Bz intragastrically (100 mg/kg). We developed and validated a high-pressure liquid chromatography with UV detector method for determination of Bz levels in whole blood. Bz levels were significantly higher and persisted for longer periods of time in the blood of young rats when compared to that of adult animals.
authors.affiliation: Centro de Investigaciones Toxicológicas, Institute of Scientific and Technological Research of the Armed Forces, Buenos Aires, Argentina.
authors.forename: Romina Fernanda
authors.initials: RF
authors.lastname: Bulffer
authors.forename: José Alberto
authors.initials: JA
authors.lastname: Castro
authors.forename: Silvia Laura
authors.initials: SL
authors.lastname: Fanelli
mesh_terms: D000367:Age Factors; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D008862:Microsomes, Liver / Q000187:drug effects*; D009593:Nitroimidazoles / Q000008:administration & dosage / Q000097:blood*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D014344:Trypanocidal Agents / Q000008:administration & dosage / Q000097:blood*
chemical_list: D009593:Nitroimidazoles; D014344:Trypanocidal Agents; C009999:benzonidazole