title: New microsatellite loci for water yam (Dioscorea alata, Dioscoreaceae) and cross-amplification for other Dioscorea species.
abstract: OBJECTIVE
  Dioscorea alata L. is one of the most widely distributed species of the genus in the humid and semihumid tropics and is associated with traditional agriculture. Only a few microsatellite markers have been developed so far for this and other Dioscorea species.
  
  
  RESULTS
  We isolated 14 codominant polymorphic microsatellite markers using a microsatellite-enriched genomic library technique. Ten microsatellite loci were selected, and 80 D. alata accessions from different regions in Brazil were evaluated with nine polymorphic loci. The polymorphism information content (PIC) varied from 0.39 to 0.78 and the power discrimination (PD) ranged from 0.15 to 0.91. Six of the markers showed transferability for the species D. bulbifera, D. cayenensis-D. rotundata, and D. trifida.
  
  
  CONCLUSIONS
  The SSR markers obtained are an important tool for further studies aiming to characterize the genetic diversity in D. alata and other Dioscorea spp. accessions.
authors.affiliation: Departamento de Genética, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, CP 83, Piracicaba, SP, Brazil, CEP 13400-970.
authors.forename: Marcos V B M
authors.initials: MV
authors.lastname: Siqueira
authors.forename: Thiago G
authors.initials: TG
authors.lastname: Marconi
authors.forename: Maria L
authors.initials: ML
authors.lastname: Bonatelli
authors.forename: Maria I
authors.initials: MI
authors.lastname: Zucchi
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Veasey
mesh_terms: D018744:DNA, Plant / Q000235:genetics / Q000302:isolation & purification; D027742:Dioscorea / Q000235:genetics*; D056426:Genetic Loci / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D013045:Species Specificity; D014867:Water
chemical_list: D018744:DNA, Plant; D014867:Water