title: An improved high-performance liquid chromatographic method for simultaneous determination of tocopherols, tocotrienols and γ-oryzanol in rice.
abstract: An improved normal phase high performance liquid chromatographic (NP-HPLC) method was developed for simultaneous quantification of eight vitamin E isomers (α-, β-, γ- and δ-tocopherols and α-, β-, γ- and δ-tocotrienols) and γ-oryzanol in rice. A complete separation of all compounds was achieved within 25 min using an Inertsil CN-3, SIL-100A 5 μM (4.6 mm × 250 mm) column and an isocratic elution system of hexane/isopropanol/ethylacetate/acetic acid (97.6:0.8:0.8:0.8, v/v/v/v) at a flow rate varying from 0.7 to 1.5 mL min(-1). A linear correlation coefficient (r(2)>0.99) and high reproducibility were obtained at concentrations ranging 0.05-10 μg mL(-1) for vitamin E isomers and 0.5-500 μg mL(-1) for γ-oryzanol. This method proved to be rapid, accurate and reproducible.
authors.affiliation: Department of Agricultural Chemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, Taiwan.
authors.forename: Shao-Hua
authors.initials: SH
authors.lastname: Huang
authors.forename: Lean-Teik
authors.initials: LT
authors.lastname: Ng
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016014:Linear Models; D012275:Oryza / Q000737:chemistry*; D010666:Phenylpropionates / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D024505:Tocopherols / Q000032:analysis*; D024508:Tocotrienols / Q000032:analysis*
chemical_list: D010666:Phenylpropionates; D024508:Tocotrienols; D024505:Tocopherols; C013172:gamma-oryzanol