title: Spinal cord injury markedly altered protein expression patterns in the affected rat urinary bladder during healing stages.
abstract: The influence of spinal cord injury (SCI) on protein expression in the rat urinary bladder was assessed by proteomic analysis at different time intervals post-injury. After contusion SCI between T9 and T10, bladder tissues were processed by 2-DE and MALDI-TOF/MS at 6 hr to 28 days after SCI to identify proteins involved in the healing process of SCI-induced neurogenic bladder. Approximately 1,000 spots from the bladder of SCI and sham groups were visualized and identified. At one day after SCI, the expression levels of three protein were increased, and seven spots were down-regulated, including heat shock protein 27 (Hsp27) and heat shock protein 20 (Hsp20). Fifteen spots such as S100-A11 were differentially expressed seven days post-injury, and seven proteins including transgelin had altered expression patterns 28 days after injury. Of the proteins with altered expression levels, transgelin, S100-A11, Hsp27 and Hsp20 were continuously and variably expressed throughout the entire post-SCI recovery of the bladder. The identified proteins at each time point belong to eight functional categories. The altered expression patterns identified by 2-DE of transgelin and S100-A11 were verified by Western blot. Transgelin and protein S100-A11 may be candidates for protein biomarkers in the bladder healing process after SCI.
authors.affiliation: MRCND and Department of Biochemistry, BK21 Program for Biomedical Sciences, School of Medicine and Institute of Health Sciences, Gyeongsang National University, Jinju, Korea.
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Lee
authors.forename: Bong Jo
authors.initials: BJ
authors.lastname: Kim
authors.forename: Gyujin
authors.initials: G
authors.lastname: Sim
authors.forename: Gyu-Tae
authors.initials: GT
authors.lastname: Kim
authors.forename: Dawon
authors.initials: D
authors.lastname: Kang
authors.forename: Jae Hun
authors.initials: JH
authors.lastname: Jung
authors.forename: Jeong Seok
authors.initials: JS
authors.lastname: Hwa
authors.forename: Yeon Ju
authors.initials: YJ
authors.lastname: Kwak
authors.forename: Yeon Jin
authors.initials: YJ
authors.lastname: Choi
authors.forename: Young Sook
authors.initials: YS
authors.lastname: Park
authors.forename: Jaehee
authors.initials: J
authors.lastname: Han
authors.forename: Cheol Soon
authors.initials: CS
authors.lastname: Lee
authors.forename: Kee Ryeon
authors.initials: KR
authors.lastname: Kang
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D050886:HSP20 Heat-Shock Proteins / Q000378:metabolism; D055551:HSP27 Heat-Shock Proteins / Q000378:metabolism; D008840:Microfilament Proteins / Q000378:metabolism; D009124:Muscle Proteins / Q000378:metabolism; D020543:Proteome / Q000096:biosynthesis*; D040901:Proteomics; D051381:Rats; D017207:Rats, Sprague-Dawley; D009418:S100 Proteins / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013119:Spinal Cord Injuries / Q000378:metabolism* / Q000473:pathology; D001743:Urinary Bladder / Q000378:metabolism*; D014945:Wound Healing
chemical_list: D015415:Biomarkers; D050886:HSP20 Heat-Shock Proteins; D055551:HSP27 Heat-Shock Proteins; D008840:Microfilament Proteins; D009124:Muscle Proteins; D020543:Proteome; D009418:S100 Proteins; C051615:transgelin