title: Characterization of an antimicrobial dental resin adhesive containing zinc methacrylate.
abstract: This study evaluates the effect of zinc methacrylate (ZM) on the degree of conversion (DC), cytotoxicity and antimicrobial activity (AA) of an experimental resin. Tetraethyleneglycol dimethacrylate was used as the experimental resin and it was photo activated using camphoroquinone and ethyl 4-dimethylamine benzoate. Additionally, 1.0, 2.5, 5.0, 10, 20 and 30 wt% of ZM was added to the various experimental resins. The DC was accessed by Fourier Transform infrared spectroscopy. For cytotoxicity, immortalized mouse fibroblasts were exposed to the experimental resin extracts. An MTT assay was used to access the cytotoxicity. The AA against Streptococcus mutans UA159 was accessed by the agar diffusion method. An improvement in the DC in all concentrations of ZM was observed. The greater the amount of ZM on the experimental resin, the less the cytotoxicity was provoked. Three ZM concentrations showed AA that exhibited various inhibition growth zones with 10, 20 (10 mm) and 30 wt% (15 mm).
authors.affiliation: Department of Operative Dentistry, CDC-Bio, School of Dentistry, Federal University of Pelotas, Rua Gonçalves Chaves, 457, Pelotas, RS, 96015-560, Brazil.
authors.forename: Sandrina
authors.initials: S
authors.lastname: Henn
authors.forename: Fernanda
authors.initials: F
authors.lastname: Nedel
authors.forename: Rodrigo Varella
authors.initials: RV
authors.lastname: de Carvalho
authors.forename: Rafael Guerra
authors.initials: RG
authors.lastname: Lund
authors.forename: Maximiliano Sérgio
authors.initials: MS
authors.lastname: Cenci
authors.forename: Tatiana
authors.initials: T
authors.lastname: Pereira-Cenci
authors.forename: Flávio Fernando
authors.initials: FF
authors.lastname: Demarco
authors.forename: Evandro
authors.initials: E
authors.lastname: Piva
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002461:Cell Line, Transformed; D003738:Dental Cements; D008689:Methacrylates / Q000737:chemistry*; D051379:Mice; D008826:Microbial Sensitivity Tests; D012117:Resins, Synthetic; D017550:Spectroscopy, Fourier Transform Infrared; D013295:Streptococcus mutans / Q000187:drug effects; D015032:Zinc / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D003738:Dental Cements; D008689:Methacrylates; D012117:Resins, Synthetic; D015032:Zinc