title: Potential effect of resistin on the ET-1-increased reactions of blood pressure in rats and Ca2+ signaling in vascular smooth muscle cells.
abstract: Resistin and endothelin-1 (ET-1) are upregulated in people with type II diabetes mellitus, central obesity, and hypertension. ET-1 signaling is involved in Ca(2+)-contraction coupling and related to blood pressure regulation. The aim of this study is to investigate the role of resistin on ET-1-increased blood pressure and Ca(2+) signaling. The blood pressure and cytosolic Ca(2+) of vascular smooth muscle cells (VSMCs) of Sprague-Dawley rats were detected. The data demonstrated that resistin accelerated and prolonged ET-1-induced increases in blood pressure and had significant effects on ET-1-increased Ca(2+) reactions. Resistin-enhanced ET-1-increased Ca(2+) reactions were reversed by blockers of store-operated Ca(2+) entry (SOCE) and extracellular-signal-regulated kinase (ERK). The endogenous expression of Orai and stromal interaction molecular (STIM) were characterized in the VSMCs. Furthermore, resistin-enhanced ET-1 Ca(2+) reactions and the resistin-dependent activation of SOCE were abolished under STIM1-siRNA treatment, indicating that STIM1 plays an important role in resistin-enhanced ET-1 Ca(2+) reactions in VSMCs. Resistin appears to exert effects on ET-1-induced Ca(2+) increases by enhancing the activity of ERK-dependent SOCE (STIM1-partcipated), and may accelerate and prolong ET-1-increased blood pressure via the same pathway.
authors.affiliation: Department and Institute of Physiology, School of Medicine, National Yang-Ming University, Taipei, Taiwan.
authors.forename: Tung-Yueh
authors.initials: TY
authors.lastname: Chuang
authors.forename: Lo-Chun
authors.initials: LC
authors.lastname: Au
authors.forename: Li-Chun
authors.initials: LC
authors.lastname: Wang
authors.forename: Low-Tone
authors.initials: LT
authors.lastname: Ho
authors.forename: De-Ming
authors.initials: DM
authors.lastname: Yang
authors.forename: Chi-Chang
authors.initials: CC
authors.lastname: Juan
mesh_terms: D000818:Animals; D001483:Base Sequence; D001794:Blood Pressure / Q000187:drug effects* / Q000502:physiology; D015220:Calcium Channels / Q000235:genetics / Q000502:physiology; D020013:Calcium Signaling / Q000187:drug effects* / Q000502:physiology; D002460:Cell Line; D019332:Endothelin-1 / Q000494:pharmacology* / Q000502:physiology; D015870:Gene Expression; D020935:MAP Kinase Signaling System; D008297:Male; D008562:Membrane Glycoproteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology; D032389:Myocytes, Smooth Muscle / Q000187:drug effects* / Q000502:physiology; D034741:RNA, Small Interfering / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D052243:Resistin / Q000494:pharmacology* / Q000502:physiology; D000071737:Stromal Interaction Molecule 1
chemical_list: D015220:Calcium Channels; D019332:Endothelin-1; D008562:Membrane Glycoproteins; D034741:RNA, Small Interfering; D052243:Resistin; C499622:Retn protein, rat; C518857:Stim1 protein, rat; D000071737:Stromal Interaction Molecule 1