title: Evolution of human immunodeficiency virus type 1 in a patient with cross-reactive neutralizing activity in serum.
abstract: Analysis of longitudinally obtained HIV-1 env sequences from an individual with reported cross-reactive neutralizing activity revealed that the majority of viral variants obtained from serum between 4 and 7 years after seroconversion were unable to persist in peripheral blood. Here we show that these viral variants were more sensitive to autologous serum neutralization, had shorter envelopes with fewer potential N-linked glycosylation sites, and showed lower replication kinetics than successfully evolving HIV-1 variants. These data reflect the host selection pressures on phenotypic characteristics of HIV-1 and illustrate in detail the dynamic interaction between HIV-1 and its host's humoral immune responses.
authors.affiliation: Department of Experimental Immunology, Academic Medical Center, AZ Amsterdam, The Netherlands.
authors.forename: Marit J
authors.initials: MJ
authors.lastname: van Gils
authors.forename: Diana
authors.initials: D
authors.lastname: Edo-Matas
authors.forename: Emma J
authors.initials: EJ
authors.lastname: Bowles
authors.forename: Judith A
authors.initials: JA
authors.lastname: Burger
authors.forename: Guillaume B
authors.initials: GB
authors.lastname: Stewart-Jones
authors.forename: Hanneke
authors.initials: H
authors.lastname: Schuitemaker
mesh_terms: D000595:Amino Acid Sequence; D057134:Antibodies, Neutralizing / Q000097:blood*; D003429:Cross Reactions; D019143:Evolution, Molecular; D015752:Genes, env; D014644:Genetic Variation; D015483:HIV Antibodies / Q000097:blood*; D015658:HIV Infections / Q000276:immunology / Q000821:virology*; D015497:HIV-1 / Q000235:genetics* / Q000276:immunology* / Q000502:physiology; D006801:Humans; D008969:Molecular Sequence Data; D020539:Sequence Analysis, Protein; D054299:env Gene Products, Human Immunodeficiency Virus / Q000737:chemistry / Q000235:genetics / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D015483:HIV Antibodies; D054299:env Gene Products, Human Immunodeficiency Virus