title: DNA barcoding of nymphalid butterflies (Nymphalidae: Lepidoptera) from Western Ghats of India.
abstract: We have checked the utility of DNA barcoding for species identification of nymphalid butterflies from Western Ghats of India by using 650 bp sequence of mitochondrial gene cytochrome c oxidase subunit I. Distinct DNA barcoding gap (i.e. difference between intraspecies and interspecies nucleotide divergence), exists between species studied here. When our sequences were compared with the sequences of the conspecifics submitted from different geographic regions, nine cases of deep intraspecies nucleotide divergences were observed. In spite of this, NJ (Neighbour Joining) clustering analysis successfully discriminated all species. Observed cases of deep intraspecies nucleotide divergences certainly warrant further study.
authors.affiliation: Department of Zoology, Modern College, Shivajinagar, Pune, 411005 Maharashtra, India.
authors.forename: S S
authors.initials: SS
authors.lastname: Gaikwad
authors.forename: H V
authors.initials: HV
authors.lastname: Ghate
authors.forename: S S
authors.initials: SS
authors.lastname: Ghaskadbi
authors.forename: M S
authors.initials: MS
authors.lastname: Patole
authors.forename: Y S
authors.initials: YS
authors.lastname: Shouche
mesh_terms: D000818:Animals; D002080:Butterflies / Q000235:genetics*; D016000:Cluster Analysis; D019295:Computational Biology / Q000379:methods; D058893:DNA Barcoding, Taxonomic / Q000379:methods*; D003576:Electron Transport Complex IV / Q000235:genetics; D014644:Genetic Variation; D007194:India; D013045:Species Specificity
chemical_list: D003576:Electron Transport Complex IV