title: PDGFRβ signaling regulates mural cell plasticity and inhibits fat development.
abstract: Mural cells (pericytes and vascular smooth muscle cells) provide trophic and structural support to blood vessels. Vascular smooth muscle cells alternate between a synthetic/proliferative state and a differentiated/contractile state, but the dynamic states of pericytes are poorly understood. To explore the cues that regulate mural cell differentiation and homeostasis, we have generated conditional knockin mice with activating mutations at the PDGFRβ locus. We show that increased PDGFRβ signaling drives cell proliferation and downregulates differentiation genes in aortic vascular smooth muscle. Increased PDGFRβ signaling also induces a battery of immune response genes in pericytes and mesenchymal cells and inhibits differentiation of white adipocytes. Mural cells are emerging as multipotent progenitors of pathophysiological importance, and we identify PDGFRβ signaling as an important in vivo regulator of their progenitor potential.
authors.affiliation: Department of Developmental and Regenerative Biology, Mount Sinai School of Medicine, New York, NY 10029, USA. lorin-olson@omrf.org
authors.forename: Lorin E
authors.initials: LE
authors.lastname: Olson
authors.forename: Philippe
authors.initials: P
authors.lastname: Soriano
mesh_terms: D052438:Adipocytes, White / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D055879:Gene Knock-In Techniques; D006706:Homeostasis; D019426:Integrases / Q000378:metabolism; D008648:Mesoderm / Q000166:cytology / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000378:metabolism*; D020286:Pericytes / Q000166:cytology* / Q000378:metabolism*; D010641:Phenotype; D020797:Receptor, Platelet-Derived Growth Factor beta / Q000502:physiology*
chemical_list: D016207:Cytokines; D020797:Receptor, Platelet-Derived Growth Factor beta; C045073:Cre recombinase; D019426:Integrases