title: A glass bead protocol for recovery of host cell free Ehrlichia canis and quantification by Sybr-green real-time PCR.
abstract: E. canis infection of the canine cell line DH82 is a routine in studies with this bacteria. A protocol for isolation of host cell free bacteria was developed based on the use of glass beads. Improvement of infection with E. canis isolated by this method was detected by real-time PCR.
authors.affiliation: Unidade de Biotecnologia, UNAERP, Ribeirão Preto, Brazil.
authors.forename: G P
authors.initials: GP
authors.lastname: Cardozo
authors.forename: E V
authors.initials: EV
authors.lastname: Santos
authors.forename: A L
authors.initials: AL
authors.lastname: Fachin
authors.forename: S C
authors.initials: SC
authors.lastname: França
authors.forename: M
authors.initials: M
authors.lastname: Marins
mesh_terms: D000818:Animals; D052160:Benzothiazoles; D002460:Cell Line; D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods*; D004269:DNA, Bacterial / Q000032:analysis; D003959:Diamines; D004283:Dog Diseases / Q000382:microbiology; D004285:Dogs; D041061:Ehrlichia canis / Q000235:genetics / Q000302:isolation & purification*; D016873:Ehrlichiosis / Q000382:microbiology; D005456:Fluorescent Dyes / Q000378:metabolism*; D005898:Glass; D006801:Humans; D009930:Organic Chemicals / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011804:Quinolines
chemical_list: D052160:Benzothiazoles; D004269:DNA, Bacterial; D003959:Diamines; D005456:Fluorescent Dyes; D009930:Organic Chemicals; D011804:Quinolines; C098022:SYBR Green I