title: Detection of characteristic metabolites of Aspergillus fumigatus and Candida species using ion mobility spectrometry-metabolic profiling by volatile organic compounds.
abstract: Volatile metabolites of Aspergillus fumigatus and Candida species can be detected by gas chromatography/mass spectrometry (GC/MS). A multi-capillary column - ion mobility spectrometer (MCC-IMS) was used in this study to assess volatile organic compounds (VOCs) in the headspace above A. fumigatus and the four Candida species Candida albicans, Candida parapsilosis, Candida glabrata and Candida tropicalis in an innovative approach, validated for A. fumigatus and C. albicans by GC/MS analyses. For the detection of VOCs, a special stainless steel measurement chamber for the microbial cultures was used. The gas outlet was either attached to MCC-IMS or to adsorption tubes (Tenax GR) for GC/MS measurements. Isoamyl alcohol, cyclohexanone, 3-octanone and phenethylalcohol can be described as discriminating substances by means of GC/MS. With MCC-IMS, the results for 3-octanone and phenethylalcohol are concordant and additionally to GC/MS, ethanol and two further compounds (p_0642_1/p_683_1 and p_705_3) can be described. Isoamyl alcohol and cyclohexanone were not properly detectable with MCC-IMS. The major advantage of the MCC-IMS system is the feasibility of rapid analysis of complex gas mixtures without pre-concentration or preparation of samples and regardless of water vapour content in an online setup. Discrimination of fungi on genus level of the investigated germs by volatile metabolic profile and therefore detection of VOC is feasible. However, a further discrimination on species level for Candida species was not possible.
authors.affiliation: Department of Anaesthesiology, Emergency and Intensive Care Medicine, University of Göttingen, Göttingen, Germany. tperl@medizin.uni-goettingen.de
authors.forename: Thorsten
authors.initials: T
authors.lastname: Perl
authors.forename: Melanie
authors.initials: M
authors.lastname: Jünger
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Vautz
authors.forename: Jürgen
authors.initials: J
authors.lastname: Nolte
authors.forename: Martin
authors.initials: M
authors.lastname: Kuhns
authors.forename: Margarete
authors.initials: M
authors.lastname: Borg-von Zepelin
authors.forename: Michael
authors.initials: M
authors.lastname: Quintel
mesh_terms: D001232:Aspergillus fumigatus / Q000145:classification / Q000378:metabolism*; D002175:Candida / Q000145:classification / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D055442:Metabolome; D009172:Mycology / Q000379:methods; D013057:Spectrum Analysis / Q000379:methods*; D055549:Volatile Organic Compounds / Q000032:analysis*
chemical_list: D055549:Volatile Organic Compounds