title: Piperine ameliorates the severity of cerulein-induced acute pancreatitis by inhibiting the activation of mitogen activated protein kinases.
abstract: Piperine is a phenolic component of black pepper (Piper nigrum) and long pepper (Piper longum), fruits used in traditional Asian medicine. Our previous study showed that piperine inhibits lipopolysaccharide-induced inflammatory responses. In this study, we investigated whether piperine reduces the severity of cerulein-induced acute pancreatitis (AP). Administration of piperine reduced histologic damage and myeloperoxidase (MPO) activity in the pancreas and ameliorated many of the examined laboratory parameters, including the pancreatic weight (PW) to body weight (BW) ratio, as well as serum levels of amylase and lipase and trypsin activity. Furthermore, piperine pretreatment reduced the production of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 during cerulein-induced AP. In accordance with in vivo results, piperine reduced cell death, amylase and lipase activity, and cytokine production in isolated cerulein-treated pancreatic acinar cells. In addition, piperine inhibited the activation of mitogen-activated protein kinases (MAPKs). These findings suggest that the anti-inflammatory effect of piperine in cerulein-induced AP is mediated by inhibiting the activation of MAPKs. Thus, piperine may have a protective effect against AP.
authors.affiliation: Department of Herbology, School of Oriental Medicine, Wonkwang University, Iksan, 540-749 Jeonbuk, South Korea.
authors.forename: Gi-Sang
authors.initials: GS
authors.lastname: Bae
authors.forename: Min-Sun
authors.initials: MS
authors.lastname: Kim
authors.forename: Jinsu
authors.initials: J
authors.lastname: Jeong
authors.forename: Hye-Youn
authors.initials: HY
authors.lastname: Lee
authors.forename: Kyoung-Chel
authors.initials: KC
authors.lastname: Park
authors.forename: Bon Soon
authors.initials: BS
authors.lastname: Koo
authors.forename: Byung-Jin
authors.initials: BJ
authors.lastname: Kim
authors.forename: Tae-Hyeon
authors.initials: TH
authors.lastname: Kim
authors.forename: Seung Ho
authors.initials: SH
authors.lastname: Lee
authors.forename: Sung-Yeon
authors.initials: SY
authors.lastname: Hwang
authors.forename: Yong Kook
authors.initials: YK
authors.lastname: Shin
authors.forename: Ho-Joon
authors.initials: HJ
authors.lastname: Song
authors.forename: Sung-Joo
authors.initials: SJ
authors.lastname: Park
mesh_terms: D000470:Alkaloids / Q000627:therapeutic use*; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use*; D017209:Apoptosis; D052117:Benzodioxoles / Q000627:therapeutic use*; D002108:Ceruletide / Q000633:toxicity; D004789:Enzyme Activation / Q000187:drug effects; D053583:Interleukin-1beta / Q000037:antagonists & inhibitors; D051379:Mice; D008810:Mice, Inbred C57BL; D020928:Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors*; D010195:Pancreatitis / Q000188:drug therapy* / Q000473:pathology; D010880:Piperidines / Q000627:therapeutic use*; D053284:Polyunsaturated Alkamides / Q000627:therapeutic use*; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors; D000093242:Interleukin-6 Inhibitors
chemical_list: D000470:Alkaloids; D000894:Anti-Inflammatory Agents, Non-Steroidal; D052117:Benzodioxoles; D002108:Ceruletide; D053583:Interleukin-1beta; D020928:Mitogen-Activated Protein Kinases; D010880:Piperidines; D053284:Polyunsaturated Alkamides; D014409:Tumor Necrosis Factor-alpha; D000093242:Interleukin-6 Inhibitors; C008922:piperine