title: Passive Ca(2+) overload in H9c2 cardiac myoblasts: assessment of cellular damage and cytosolic Ca(2+) transients.
abstract: Increase of resting Ca(2+) levels and amplitude of vasopressin-induced Ca(2+) transients were observed when cells in serum-free medium were exposed to 5mM Ca(2+) for 2h. Small effect on cell viability was also observed. A rapid cytotoxic effect was developed in the presence of 10mM Ca(2+) and absence of serum. However, cells exposed to 10mM Ca(2+) in the presence of serum were protected from damage for at least 2days. Resting Ca(2+) levels and cytosolic Ca(2+) transients in serum-containing medium with 10mM Ca(2+) displayed lower increases and a tendency to recover control values. When serum was absent, cells preincubated with 10mM Ca(2+) were more sensitive to thapsigargin-induced damage than cells preincubated with lower Ca(2+). The sensitivity was similar when serum was present. Tolerance to high Ca(2+) in the presence of serum was linked to potentiation of the mitochondrial Ca(2+) entry to decrease the sarcoplasmic reticulum Ca(2+) overload.
authors.affiliation: Departamento de Bioquímica y Biología Molecular A, Universidad de Murcia, Campus de Espinardo, Murcia, Spain.
authors.forename: Fernando
authors.initials: F
authors.lastname: Soler
authors.forename: Antonio
authors.initials: A
authors.lastname: Lax
authors.forename: M
authors.initials: M
authors.lastname: Carmen Asensio
authors.forename: Domingo
authors.initials: D
authors.lastname: Pascual-Figal
authors.forename: Francisco
authors.initials: F
authors.lastname: Fernández-Belda
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism* / Q000494:pharmacology; D020013:Calcium Signaling / Q000187:drug effects / Q000502:physiology; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D003470:Culture Media; D016895:Culture Media, Serum-Free; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D008929:Mitochondria, Heart / Q000187:drug effects / Q000378:metabolism; D008955:Models, Cardiovascular; D032386:Myoblasts, Cardiac / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D012508:Sarcolemma / Q000187:drug effects / Q000378:metabolism; D012519:Sarcoplasmic Reticulum / Q000187:drug effects / Q000378:metabolism; D019284:Thapsigargin / Q000633:toxicity
chemical_list: D003470:Culture Media; D016895:Culture Media, Serum-Free; D019284:Thapsigargin; D002118:Calcium