title: [NADP increases the level of histone H2AX phosphorylation in mouse heart cells after ionizing radiation].
abstract: Phosphorylation of replaceable histone H2AX occurs in megabase chromatin domains around DNA double-strand breaks (DSBs), and this modification called gamma-H2AX can be used as an effective marker for DSBs repair and DNA damage response. Using Western blotting and immunohistochemistry techniques we have studied here the influence of exogenous nicotinamide adenine dinucleotide phosphate (NADP) which could potentially increase the intracellular level of NAD+ and on the level of gamma-H2AX formation in mouse heart cells after ionizing radiation (IR). We have found that injection of NAD+ in different doses immediately after IR causes an increased level of gamma-H2AX in mouse heart cells 20 min after IR at the dose of 3 Gy compared to control mice after IR exposure. It indicates that it could be a relationship between intracellular NAD+ content and DNA damage response in vivo.
authors.forename: D V
authors.initials: DV
authors.lastname: Firsanov
authors.forename: A V
authors.initials: AV
authors.lastname: Kropotov
authors.forename: V M
authors.initials: VM
authors.lastname: Mikhaĭlov
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002843:Chromatin / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D004247:DNA / Q000378:metabolism; D053903:DNA Breaks, Double-Stranded / Q000528:radiation effects; D015870:Gene Expression; D006321:Heart / Q000528:radiation effects; D006657:Histones / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D007150:Immunohistochemistry; D007274:Injections, Intraperitoneal; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009243:NAD / Q000378:metabolism*; D009249:NADP / Q000378:metabolism* / Q000494:pharmacology; D010766:Phosphorylation / Q000528:radiation effects; D011839:Radiation, Ionizing; D014916:Whole-Body Irradiation / Q000009:adverse effects
chemical_list: D002843:Chromatin; D004247:DNA; D006657:Histones; D009243:NAD; D009249:NADP; C475961:gamma-H2AX protein, mouse