title: BRG1 is indispensable for IFN-γ-induced TRIM22 expression, which is dependent on the recruitment of IRF-1.
abstract: The modification of chromatin structure is increasingly recognized to be an important facet of transcriptional regulation. Here, we report that Brahma-related gene 1 (BRG1), a chromatin remodeling enzyme, plays a crucial role in IFN-γ-induced TRIM22 expression. Our results showed that IFN-γ failed to induce TRIM22 expression in BRG1-deficient SW-13 cells, and reconstitution of BRG1 in this cell line could restore IFN-γ induction of TRIM22. Furthermore, it was revealed that BRG1 absence, per se, did not impair IFN-γ-induced IRF-1 expression, but blocked its access to TRIM22 promoter, and BRG1-dependent induction of TRIM22 perfectly correlated with BRG1-dependent recruitment of IRF-1 to TRIM22 promoter. We also found that the DNA-dependent ATPase domain of BRG1 was required for TRIM22 expression and IRF-1 recruitment in response to IFN-γ stimulation, suggesting that BRG1-mediated chromatin remodeling is critical for the IFN-γ-inducibility of TRIM22 gene.
authors.affiliation: Institute for Immunobiology, Department of Immunology, Shanghai Medical College of Fudan University, Shanghai 200032, PR China.
authors.forename: Yaxin
authors.initials: Y
authors.lastname: Wang
authors.forename: Bo
authors.initials: B
authors.lastname: Gao
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.forename: Sidong
authors.initials: S
authors.lastname: Xiong
mesh_terms: D045744:Cell Line, Tumor; D002843:Chromatin / Q000378:metabolism; D004265:DNA Helicases / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D050856:Interferon Regulatory Factor-1 / Q000378:metabolism*; D007371:Interferon-gamma / Q000378:metabolism* / Q000494:pharmacology; D015778:Minor Histocompatibility Antigens; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D000070584:Tripartite Motif Proteins
chemical_list: D002843:Chromatin; D050856:Interferon Regulatory Factor-1; D015778:Minor Histocompatibility Antigens; D009687:Nuclear Proteins; D012097:Repressor Proteins; C094314:TRIM22 protein, human; D014157:Transcription Factors; D000070584:Tripartite Motif Proteins; D007371:Interferon-gamma; C084108:SMARCA4 protein, human; D004265:DNA Helicases