title: Filtration kinetics of chitosan separation by electrofiltration.
abstract: Downstream processing of chitosan requires several technological steps that contribute to the total production costs. Precipitation and especially evaporation are energy-consuming processes, resulting in higher costs and limiting industrial scale production. This study investigated the filtration kinetics of chitosan derived from cell walls of fungi and from exoskeletons of arthropods by electrofiltration, an alternative method, thus reducing the downstream processing steps and costs. Experiments with different voltages and pressures were conducted in order to demonstrate the effect of both parameters on filtration kinetics. The concentration of the biopolymer was obtained by the average factor of 40 by applying an electric field of 4 V/mm and pressure of 4 bars. A series of analytical experiments demonstrated the lack of structural and functional changes in chitosan molecules after electrofiltration. These results, combined with the reduction of energy and processing time, define the investigated method as a promising downstream step in the chitosan production technology.
authors.affiliation: Institute of Process Engineering in Life Sciences, Bioprocess Engineering, Karlsruhe Institute of Technology, Karlsruhe, Germany. goezde.goezke@kit.edu
authors.forename: Gözde
authors.initials: G
authors.lastname: Gözke
authors.forename: Frank
authors.initials: F
authors.lastname: Kirschhöfer
authors.forename: Stefan
authors.initials: S
authors.lastname: Heissler
authors.forename: Mirko
authors.initials: M
authors.lastname: Trutnau
authors.forename: Gerald
authors.initials: G
authors.lastname: Brenner-Weiss
authors.forename: Jelka
authors.initials: J
authors.lastname: Ondruschka
authors.forename: Ursula
authors.initials: U
authors.lastname: Obst
authors.forename: Clemens
authors.initials: C
authors.lastname: Posten
mesh_terms: D020095:Absidia / Q000737:chemistry; D000818:Animals; D001709:Biotechnology / Q000379:methods*; D003386:Brachyura / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods*; D004574:Electromagnetic Fields; D005374:Filtration / Q000295:instrumentation / Q000379:methods*; D005947:Glucose / Q000378:metabolism; D019216:Metals, Heavy / Q000737:chemistry; D009089:Mucor / Q000737:chemistry; D011312:Pressure; D011506:Proteins / Q000032:analysis; D013059:Spectrum Analysis, Raman; D014783:Viscosity
chemical_list: D019216:Metals, Heavy; D011506:Proteins; D048271:Chitosan; D005947:Glucose