title: Suppression of ovalbumin-induced allergic diarrhea by diminished intestinal peristalsis in RAMP1-deficient mice.
abstract: Recent studies have revealed that various neurotransmitters regulate the immune system via their receptors expressed on the immune cells. Calcitonin gene-related peptide (CGRP), a sensory nerve C-fiber neuropeptide, is also known to have the ability to modulate the functions of immune cells in vitro. However, the contribution of CGRP to the immune regulation in vivo remains to be fully elucidated. Here we report that mice deficient in receptor activity-modifying protein 1 (RAMP1), which is a subunit of the CGRP receptor, showed a significantly lower incidence of diarrhea compared with wild-type (WT) mice in the ovalbumin (OVA)-induced food allergic model. Serum OVA-specific IgE levels and the differentiation of T helper cells was comparable in WT mice and RAMP1-deficient mice. Moreover, there were no significant differences between recruitment and degranulation of mast cells in the small intestine of these mice. In contrast, significantly diminished intestinal peristalsis was observed by the allergy induction in RAMP1-deficient mice compared with WT mice. These results suggest that this suppression of allergic diarrhea is due to the diminished intestinal peristalsis in RAMP1-deficient mice.
authors.affiliation: Department of Immunology, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Rintaro
authors.initials: R
authors.lastname: Yoshikawa
authors.forename: Norihisa
authors.initials: N
authors.lastname: Mikami
authors.forename: Ippei
authors.initials: I
authors.lastname: Otani
authors.forename: Taeko
authors.initials: T
authors.lastname: Kishimoto
authors.forename: Satoshi
authors.initials: S
authors.lastname: Nishioka
authors.forename: Nagisa
authors.initials: N
authors.lastname: Hashimoto
authors.forename: Yayoi
authors.initials: Y
authors.lastname: Miyagi
authors.forename: Yutaro
authors.initials: Y
authors.lastname: Takuma
authors.forename: Kaori
authors.initials: K
authors.lastname: Sueda
authors.forename: So-ichiro
authors.initials: S
authors.lastname: Fukada
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yamamoto
authors.forename: Kazutake
authors.initials: K
authors.lastname: Tsujikawa
mesh_terms: D000818:Animals; D003967:Diarrhea / Q000235:genetics / Q000276:immunology* / Q000503:physiopathology; D005512:Food Hypersensitivity / Q000235:genetics / Q000276:immunology* / Q000503:physiopathology; D007422:Intestines / Q000276:immunology* / Q000503:physiopathology; D051379:Mice; D008817:Mice, Mutant Strains; D010047:Ovalbumin / Q000276:immunology; D010528:Peristalsis / Q000235:genetics / Q000276:immunology*; D058262:Receptor Activity-Modifying Protein 1 / Q000235:genetics / Q000276:immunology*
chemical_list: C545505:Ramp1 protein, mouse; D058262:Receptor Activity-Modifying Protein 1; D010047:Ovalbumin