title: Covalent cell surface functionalization of human fetal osteoblasts for tissue engineering.
abstract: The chemical functionalization of cell-surface proteins of human primary fetal bone cells with hydrophilic bioorthogonal intermediates was investigated. Toward this goal, chemical pathways were developed for click reaction-mediated coupling of alkyne derivatives with cellular azido-expressing proteins. The incorporation via a tetraethylene glycol linker of a dipeptide and a reporter biotin allowed the proof of concept for the introduction of cell-specific peptide ligands and allowed us to follow the reaction in living cells. Tuning the conditions of the click reaction resulted in chemical functionalization of living human fetal osteoblasts with excellent cell survival.
authors.affiliation: Institute of Chemical Sciences and Engineering, EPFL, CH-1015, Lausanne, Switzerland.
authors.forename: Françoise
authors.initials: F
authors.lastname: Borcard
authors.forename: Aurélien
authors.initials: A
authors.lastname: Godinat
authors.forename: Davide
authors.initials: D
authors.lastname: Staedler
authors.forename: Horacio Comas
authors.initials: HC
authors.lastname: Blanco
authors.forename: Anne-Laure
authors.initials: AL
authors.lastname: Dumont
authors.forename: Catherine
authors.initials: C
authors.lastname: Chapuis-Bernasconi
authors.forename: Corinne
authors.initials: C
authors.lastname: Scaletta
authors.forename: Lee Ann
authors.initials: LA
authors.lastname: Applegate
authors.forename: Franziska Krauss
authors.initials: FK
authors.lastname: Juillerat
authors.forename: Urs T
authors.initials: UT
authors.lastname: Gonzenbach
authors.forename: Sandrine
authors.initials: S
authors.lastname: Gerber-Lemaire
authors.forename: Lucienne
authors.initials: L
authors.lastname: Juillerat-Jeanneret
mesh_terms: D000480:Alkynes / Q000737:chemistry*; D002462:Cell Membrane / Q000737:chemistry; D002470:Cell Survival; D002478:Cells, Cultured; D057930:Click Chemistry; D005333:Fetus / Q000166:cytology; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008565:Membrane Proteins / Q000737:chemistry*; D010006:Osteoblasts / Q000737:chemistry / Q000166:cytology*; D023822:Tissue Engineering / Q000379:methods
chemical_list: D000480:Alkynes; D008565:Membrane Proteins