title: Role of alkaline phosphatase in insecticidal action of Cry1Ac against Helicoverpa armigera larvae.
abstract: Cry1Ac δ-endotoxin produced by Bacillus thuringiensis (Bt) is used as a bio-pesticide for the control of Helicoverpa armigera. Aminopeptidases N (APN) and alkaline phosphatase (ALP) play critical roles in its action against H. armigera larvae. The binding of Cry1Ac with brush border membrane vesicle (BBMV) proteins was increased with the larval development although the sensitivity of larvae to δ-endotoxins decreased. There was higher expression of ALP than APN in early instar larvae with a ~10-fold higher affinity of Cry1Ac towards ALP than to APN. Binding to a specific receptor is therefore more important for the insecticidal activity rather than overall binding to the BBMV proteins. ALP might play a major role in toxicity as compared to APN.
authors.affiliation: National Botanical Research Institute, Council of Scientific and Industrial Research, Rana Pratap Marg, Lucknow, UP 226001, India.
authors.forename: Santosh K
authors.initials: SK
authors.lastname: Upadhyay
authors.forename: Pradhyumna K
authors.initials: PK
authors.lastname: Singh
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000378:metabolism / Q000494:pharmacology*; D018826:CD13 Antigens / Q000378:metabolism; D004731:Endotoxins / Q000378:metabolism / Q000494:pharmacology*; D006460:Hemolysin Proteins / Q000378:metabolism / Q000494:pharmacology*; D019476:Insect Proteins / Q000378:metabolism; D007306:Insecticides / Q000378:metabolism / Q000494:pharmacology*; D007700:Kinetics; D007814:Larva / Q000187:drug effects / Q000201:enzymology; D007915:Lepidoptera / Q000187:drug effects* / Q000201:enzymology; D008871:Microvilli / Q000201:enzymology / Q000378:metabolism; D011485:Protein Binding; D016019:Survival Analysis
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; D019476:Insect Proteins; D007306:Insecticides; C030956:insecticidal crystal protein, Bacillus Thuringiensis; D000469:Alkaline Phosphatase; D018826:CD13 Antigens