title: DNA manipulation with elastomeric nanostructures fabricated by soft-moulding of a FIB-patterned stamp.
abstract: A Focused Ion Beam (FIB)-patterned silicon mould is used to fabricate elastomeric nanostructures, whose cross-section can be dynamically and reversibly tuned by applying a controlled mechanical stress. Direct-write, based on FIB milling, allows the fabrication of nanostructures with a variety of different geometries, aspect ratio, spacing and distribution offering a higher flexibility compared to other nanopatterning approaches. Moreover, a simple double replication process based on poly(dimethylsiloxane) permits a strong reduction of the fabrication costs that makes this approach well-suited for the production of low cost nanofluidic devices. DNA stretching and single molecule manipulation capabilities of these platforms have been successfully demonstrated.
authors.affiliation: Nanomed Labs, Dipartimento di Fisica, Università di Genova, via Dodecaneso 33, 16146 Genova, Italy. elena.angeli@unige.it
authors.forename: Elena
authors.initials: E
authors.lastname: Angeli
authors.forename: Chiara
authors.initials: C
authors.lastname: Manneschi
authors.forename: Luca
authors.initials: L
authors.lastname: Repetto
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Firpo
authors.forename: Ugo
authors.initials: U
authors.lastname: Valbusa
mesh_terms: D010582:Bacteriophage lambda / Q000737:chemistry*; D004279:DNA, Viral / Q000737:chemistry*; D004129:Dimethylpolysiloxanes; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods; D049329:Nanostructures; D012826:Silicone Elastomers
chemical_list: D004279:DNA, Viral; D004129:Dimethylpolysiloxanes; D012826:Silicone Elastomers; C013830:baysilon