title: Comparative activities of daptomycin and several agents against staphylococcal blood isolates. Glycopeptide tolerance.
abstract: The activity of daptomycin was evaluated against 702 staphylococcal blood isolates (316 methicillin-susceptible Staphylococcus aureus, 187 methicillin-resistant S. aureus [MRSA], and 199 coagulase-negative staphylococci [CoNS]) collected in 41 Spanish hospitals. Glycopeptide tolerance and the incidence of heterogeneous glycopeptide-intermediate (hGISA) isolates were also examined. Vancomycin MICs determined by the Etest were compared with those obtained by the reference broth microdilution method. Daptomycin exhibited good activity, and only 2 isolates were nonsusceptible to this antibiotic. Resistance to linezolid was observed in 2 MRSA isolates and in 16 CoNS. The cfr gene was detected in 7 of these 18 isolates. Vancomycin and teicoplanin tolerance was 9.6% and 21.9%, respectively, in MRSA isolates. We detected the hGISA phenotype in 5.8% of MRSA isolates. Vancomycin MICs by the Etest were slightly higher than those obtained by broth microdilution. Daptomycin retained activity against isolates that were not susceptible to linezolid, teicoplanin, or quinupristin-dalfopristin.
authors.affiliation: Servicio de Microbiología Clínica, Hospital Clínico San Carlos, Madrid, Spain
authors.forename: Juan J
authors.initials: JJ
authors.lastname: Picazo
authors.forename: Carmen
authors.initials: C
authors.lastname: Betriu
authors.forename: Iciar
authors.initials: I
authors.lastname: Rodríguez-Avial
authors.forename: Esther
authors.initials: E
authors.lastname: Culebras
authors.forename: Fátima
authors.initials: F
authors.lastname: López-Fabal
authors.forename: María
authors.initials: M
authors.lastname: Gómez
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D016470:Bacteremia / Q000382:microbiology*; D017576:Daptomycin / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D006020:Glycopeptides / Q000494:pharmacology*; D006761:Hospitals; D006801:Humans; D008826:Microbial Sensitivity Tests; D013030:Spain; D013203:Staphylococcal Infections / Q000382:microbiology*; D013210:Staphylococcus / Q000187:drug effects* / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents; D006020:Glycopeptides; D017576:Daptomycin