title: Development of a method for the determination of glycine in human cerebrospinal fluid using pre-column derivatization and LC-MS/MS.
abstract: An LC-MS/MS method using pre-column derivatization with phenylisothiocyanate (PITC) was developed to quantify glycine in human cerebrospinal fluid (CSF) and applied to the determination of glycine in human samples collected during clinical testing. The calibration curve range for the assay was 50-10,000 ng/mL and ¹³C₂¹⁵N-glycine was used as an internal standard. Artificial CSF was used as a surrogate matrix for standards due to the presence of endogenous glycine in human CSF and this approach was validated with additional experiments involving either standard addition, or stable labeled glycine as an alternate calibration standard for endogenous glycine. Interday bias (% RE) and precision (% CV) were -4.2 and 12.3% at the LLOQ, and less than ±0.9 and 8.3% for higher concentrations, respectively. Glycine was stable in artificial CSF for at least 5h at room temperature, 55 days at -70 °C (-60 to -80 °C range), and through three freeze-thaw cycles.
authors.affiliation: Pharmacokinetics & Drug Metabolism, Amgen, Inc., One Amgen Center Drive, Thousand Oaks, CA 91320, USA.
authors.forename: Sarah F
authors.initials: SF
authors.lastname: Wilson
authors.forename: Christopher A
authors.initials: CA
authors.lastname: James
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Zhu
authors.forename: Mike T
authors.initials: MT
authors.lastname: Davis
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Rose
mesh_terms: D015415:Biomarkers / Q000134:cerebrospinal fluid; D002138:Calibration; D002853:Chromatography, Liquid / Q000592:standards; D003080:Cold Temperature; D005615:Freezing; D005998:Glycine / Q000134:cerebrospinal fluid* / Q000737:chemistry; D006801:Humans; D017879:Isothiocyanates / Q000737:chemistry; D015394:Molecular Structure; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000592:standards; D013997:Time Factors
chemical_list: D015415:Biomarkers; D017879:Isothiocyanates; C005441:phenylisothiocyanate; D005998:Glycine