title: Production of a novel bioflocculant MNXY1 by Klebsiella pneumoniae strain NY1 and application in precipitation of cyanobacteria and municipal wastewater treatment.
abstract: OBJECTIVE
  To isolate and characterize the novel bioflocculant-producing bacteria, to optimize the bioflocculant production and to evaluate its potential applications.
  
  
  RESULTS
  Klebsiella pneumoniae strain NY1, a bacterium that produces a novel bioflocculant (MNXY1), was selected on the chemically defined media. It was classified according to the 16S rRNA gene sequence, morphological and microscopic characteristics. MNXY1 was characterized to contain 26% protein and 66% total sugar. The constituent sugar monomers of MNXY1, revealed by NMR analysis, are glucose, galactose and quinovose. Favourable culture conditions for MNXY1 production were determined. Strain NY1 produces a high level (14.9 g l(-1)) of MNXY1. MNXY1 is thermostable and tolerant to the extreme pH. It precipitated 54% of cyanobacteria from laboratory culture and 72% of the total suspended solids from raw wastewater.
  
  
  CONCLUSIONS
  Strain NY1 was identified to produce a novel bioflocculant MNXY1. The outstanding performance of MNXY1 in practical applications and its availability in copious amounts make it attractive for further investigation and development for industrial scale applications.
  
  
  CONCLUSIONS
  This is first report for the identification of a quinovose-containing bioflocculant and application of a protein-polysaccharide complex bioflocculant in precipitation of cyanobacteria. These findings suggest that MNXY1 holds great potential for use in management of harmful algae and city wastewater treatment.
authors.affiliation: School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an, Shaanxi Province, China.
authors.forename: M
authors.initials: M
authors.lastname: Nie
authors.forename: X
authors.initials: X
authors.lastname: Yin
authors.forename: J
authors.initials: J
authors.lastname: Jia
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: S
authors.initials: S
authors.lastname: Liu
authors.forename: Q
authors.initials: Q
authors.lastname: Shen
authors.forename: P
authors.initials: P
authors.lastname: Li
authors.forename: Z
authors.initials: Z
authors.lastname: Wang
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D002241:Carbohydrates / Q000096:biosynthesis* / Q000302:isolation & purification; D000458:Cyanobacteria / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000235:genetics; D005427:Flocculation; D006863:Hydrogen-Ion Concentration; D007711:Klebsiella pneumoniae / Q000737:chemistry* / Q000235:genetics / Q000254:growth & development; D008970:Molecular Weight; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013696:Temperature; D014865:Waste Disposal, Fluid
chemical_list: D001426:Bacterial Proteins; D002241:Carbohydrates; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S