title: The neuroprotective effect of 2-oxoglutarate in the experimental ischemia of hippocampus.
abstract: In this study we investigated the potential neuroprotective effect of 2-oxoglutarate (2-OG) on the hippocampus in the transient vessel occlusion ischemia model in the Mongolian gerbil. The morphological and biochemical studies were performed at 7 days after occlusion of carotid arteries. The acute reduction of NeuN-positive neurons in the CA1 pyramidal layer of the hippocampus was accompanied by increased staining intensity for GFAP-positive astrocytes, indicative of glial reaction. The neuron death in the CA1 area coincided with a strong 2.4 fold decrease in the membrane forms of neuronal cell adhesion molecules and elevated levels of astrocyte-specific proteins (soluble GFAP to 2,6 times; filament GFAP to 1,5 times; calcium-binding protein S-100b to 1,6 times). Treatment with 2-oxoglutarate (2.28 g/l drinking water) for between 7 and 21 days attenuated the neuronal death and reactive astrogliosis in this model of experimental ischemia by 20-50%. Our results suggest that 2-OG may prevent the disturbances of neural cells that usually take place during ischemic pathology.
authors.affiliation: Department of Cytology, Bogomoletz Institute of Physiology, Kiev, Ukraine.
authors.forename: T N
authors.initials: TN
authors.lastname: Kovalenko
authors.forename: G A
authors.initials: GA
authors.lastname: Ushakova
authors.forename: I
authors.initials: I
authors.lastname: Osadchenko
authors.forename: G G
authors.initials: GG
authors.lastname: Skibo
authors.forename: S G
authors.initials: SG
authors.lastname: Pierzynowski
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002545:Brain Ischemia / Q000473:pathology* / Q000517:prevention & control*; D056547:CA1 Region, Hippocampal / Q000187:drug effects* / Q000473:pathology*; D005849:Gerbillinae; D007656:Ketoglutaric Acids / Q000008:administration & dosage*; D018696:Neuroprotective Agents / Q000008:administration & dosage*; D011897:Random Allocation
chemical_list: D007656:Ketoglutaric Acids; D018696:Neuroprotective Agents