title: Use of cryopreserved autologous cells for extracorporeal photochemotherapy: clinical applications.
abstract: BACKGROUND
  Using autologous cryopreserved mononuclear cells (MNCs) for extracorporeal photochemotherapy (ECP) offers several advantages, such as treating patients from geographically distant care centers or maintaining ECP schedule while dramatically reducing number of apheresis sessions. We previously reported that cryopreserved cells retain their immunomodulatory properties when exposed to UVA and psoralen. To date, there are no clinical data on the use of cryopreserved MNCs for ECP ("cryo-ECP").
  
  
  METHODS
  Three patients were treated by cryo-ECP for refractory dermatomyositis, juvenile localized scleroderma, and acute graft-versus-host disease. For the first two patients, cryo-ECP aimed to reduce the number of apheresis sessions. Each cell product was split into three equal fractions: one was infused, and the other two were frozen for later infusion. The third patient was referred to our center from a hospital 700 km away. Fifteen apheresis procedures were performed during his stay: 12 were immediately treated and infused while three were cryopreserved. After discharge, the three cryopreserved bags were thawed, ECP-treated, and then sent back to the patient.
  
  
  CONCLUSIONS
  In all three patients, cryo-ECP was safe and feasible. These cases illustrate promising clinical applications of the technique, opening perspectives for making ECP much more acceptable to patients while extending its indications.
authors.affiliation: Centre Régional de Cancérologie et Thérapie Cellulaire Pédiatrique, INSERM CIC-501, Dermatologie, and the Laboratoire d'Hématologie et Immunologie, CHU Clermont-Ferrand, Clermont-Ferrand, France. e_merlin@chu-clermontferrand.fr
authors.forename: Etienne
authors.initials: E
authors.lastname: Merlin
authors.forename: Florence
authors.initials: F
authors.lastname: Jacomet
authors.forename: Michel
authors.initials: M
authors.lastname: D'Incan
authors.forename: Pascale
authors.initials: P
authors.lastname: Halle
authors.forename: Marc
authors.initials: M
authors.lastname: Berger
authors.forename: Virginie
authors.initials: V
authors.lastname: Gandemer
authors.forename: Chirstophe
authors.initials: C
authors.lastname: Piguet
authors.forename: Pierre
authors.initials: P
authors.lastname: Souteyrand
authors.forename: François
authors.initials: F
authors.lastname: Deméocq
authors.forename: Justyna
authors.initials: J
authors.lastname: Kanold
mesh_terms: D002648:Child; D002675:Child, Preschool; D015925:Cryopreservation / Q000379:methods*; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000637:transplantation; D008297:Male; D017893:Photopheresis / Q000379:methods*; D014182:Transplantation, Autologous / Q000379:methods*