title: Polymorphisms in follicle stimulation hormone receptor, inhibin alpha, inhibin bata A, and prolactin genes, and their association with sperm quality in Chinese Holstein bulls.
abstract: Candidate genes follicle stimulation hormone receptor (FSHR), inhibin alpha (INHA), inhibin bata A (INHBA) and prolactin (PRL) were investigated for their association with sperm quality traits of semen volume per ejaculate (VOL), sperm concentration (SCON), motility (MOT), sperm motility in frozen semen (FMOT), acrosome integrity rate (AIR) and abnormal sperm rate (ASR) in a total of 123 normal mature Holstein bulls. Three reported single nucleotide polymorphisms (SNPs) of FSHR A-234500T (rs43676359), IHNA A192G (rs41257116), and IHNBA C7639T (rs43408735), and 3 novel SNPs (G7550A, C7661T, and T8370C) in exons 4 and 5 of bovine PRL gene (NC 007324) were analyzed. Analysis of variance revealed that FSHR A-234500T and INHBA C7639T polymorphisms significantly associated with VOL (P<0.05) and SCON (P<0.05), and the polymorphism of INHBA C7639T also had significant effects on MOT (P<0.05). Furthermore, the INHA A192G polymorphism significantly associated with AIR (P<0.05). No significant differences were found between the polymorphisms of PRL gene and sperm quality traits.
authors.affiliation: China Education Ministry's Key Laboratory in Agricultural Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Sang
authors.forename: Qing-Zhi
authors.initials: QZ
authors.lastname: Du
authors.forename: Wu-Cai
authors.initials: WC
authors.lastname: Yang
authors.forename: Ke-Qiong
authors.initials: KQ
authors.lastname: Tang
authors.forename: Jun-Na
authors.initials: JN
authors.lastname: Yu
authors.forename: Gua-hua
authors.initials: GH
authors.lastname: Hua
authors.forename: Xiao-Xia
authors.initials: XX
authors.lastname: Zhang
authors.forename: Li-Guo
authors.initials: LG
authors.lastname: Yang
mesh_terms: D000818:Animals; D002417:Cattle / Q000235:genetics*; D005787:Gene Frequency / Q000235:genetics; D005838:Genotype; D028322:Inhibin-beta Subunits / Q000235:genetics*; D007265:Inhibins / Q000235:genetics*; D008297:Male; D011110:Polymorphism, Genetic / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D011388:Prolactin / Q000235:genetics*; D019655:Quantitative Trait, Heritable; D011962:Receptors, FSH / Q000235:genetics*; D012661:Semen / Q000378:metabolism; D013081:Sperm Motility / Q000235:genetics; D013094:Spermatozoa / Q000378:metabolism*
chemical_list: D011962:Receptors, FSH; C422362:inhibin A; C103008:inhibin beta A subunit; D007265:Inhibins; D011388:Prolactin; D028322:Inhibin-beta Subunits