title: Recombinant BMP 4/7 fusion protein induces differentiation of bone marrow stem cells.
abstract: Bone morphogenetic proteins (BMPs) induce differentiation of mesenchymal cells to cartilage and bone. We cloned BMP4 and BMP7 cDNAs from human placenta and fetal cartilage cells, respectively, and used an Escherichia coli expression system to produce recombinant BMP4 and BMP4/7 proteins. Differentiation of primary cultures of bone marrow stem cells (BMSC) treated with BMP4 or BMP4/7 was evaluated by Von Kossa staining and by determining alkaline phosphatase activity and osteocalcin level. BMP4/7-induced BMSC differentiation more potently than BMP4. We showed that BMP4/7 fusion protein expressed in E. coli is biologically active and is a novel strategy to treat bone injury in a clinical setting.
authors.affiliation: Department of Orthopedics, The First Hospital of Harbin Medical University, 150001 Harbin, China.
authors.forename: Shaohui
authors.initials: S
authors.lastname: Yuan
authors.forename: Qi
authors.initials: Q
authors.lastname: Pan
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Binqi
authors.initials: B
authors.lastname: Wu
authors.forename: Xiguang
authors.initials: X
authors.lastname: Han
authors.forename: Zhenggang
authors.initials: Z
authors.lastname: Bi
mesh_terms: D001854:Bone Marrow Cells / Q000166:cytology* / Q000187:drug effects*; D055415:Bone Morphogenetic Protein 4 / Q000235:genetics / Q000378:metabolism*; D055419:Bone Morphogenetic Protein 7 / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D005434:Flow Cytometry; D006801:Humans; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism*
chemical_list: D055415:Bone Morphogenetic Protein 4; D055419:Bone Morphogenetic Protein 7; D011993:Recombinant Fusion Proteins