title: Photoirradiation of polycyclic aromatic hydrocarbon diones by UVA light leading to lipid peroxidation.
abstract: Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous genotoxic environmental pollutants and potentially pose a health risk to humans. In most if not all cases, PAHs in the environment can be oxidized into their corresponding PAH-diones. This process is considered a detoxification pathway with regard to tumorigenicity. Nevertheless, photo-induced toxicological activity of PAH-diones has not been systematically investigated. In this study, we show that 27 potential environmental PAH-diones induced lipid peroxidation, in a dose (light) response manner, when irradiated with UVA at 7 and 21 J cm(-2). Photoirradiation in the presence of sodium azide, deuterated methanol, or superoxide dismutase revealed that lipid peroxidation is mediated by reactive oxygen species. Electron spin resonance (ESR) spin trapping studies supported this observation. These results suggest that UVA photoirradiation of PAH-diones generates reactive oxygen species and induces lipid peroxidation.
authors.affiliation: National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR 72079, USA. yuewei.zhao@fda.hhs.gov
authors.forename: Yuewei
authors.initials: Y
authors.lastname: Zhao
authors.forename: Qingsu
authors.initials: Q
authors.lastname: Xia
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Yin
authors.forename: Hongtao
authors.initials: H
authors.lastname: Yu
authors.forename: Peter P
authors.initials: PP
authors.lastname: Fu
mesh_terms: D005609:Free Radicals / Q000737:chemistry; D008041:Linoleic Acids / Q000737:chemistry; D015227:Lipid Peroxidation / Q000528:radiation effects; D009153:Mutagens / Q000737:chemistry / Q000633:toxicity*; D055668:Photochemical Processes; D011084:Polycyclic Aromatic Hydrocarbons / Q000737:chemistry / Q000633:toxicity*; D026082:Singlet Oxygen / Q000737:chemistry; D013481:Superoxides / Q000737:chemistry; D014466:Ultraviolet Rays
chemical_list: D005609:Free Radicals; D008041:Linoleic Acids; D009153:Mutagens; D011084:Polycyclic Aromatic Hydrocarbons; D013481:Superoxides; D026082:Singlet Oxygen; C005575:methyl linoleate