title: High-performance liquid chromatographic determination and pharmacokinetic study of apigenin-7-O-β-D-glucoside in rat plasma after intravenous administration.
abstract: The present study was designed to validate an analytical method based on HPLC for the quantitative measurement of the Apigenin-7-O-β-D-glucoside (AGL) in rat plasma after intravenous administration. HPLC analysis was done using a C18 column, UV detection at 335 nm, and a mobile phase of methanol:0.2% phosphoric acid (1:1, v/v). Good linearity was observed over the range of 0.06-7.20 μg/mL with a lower limit of quantication of 0.06 μg/mL. The intra-and inter-day precision values were below 9.97%. No interference peaks or matrix effects were observed. The method was fully validated and successfully applied to a pharmacokinetic study of AGL.
authors.affiliation: School of Pharmaceutical Sciences, China Medical University, Shenyang, Liaoning Province 110001, China.
authors.forename: Zaixing
authors.initials: Z
authors.lastname: Chen
authors.forename: Xixiang
authors.initials: X
authors.lastname: Ying
authors.forename: Shu
authors.initials: S
authors.lastname: Meng
authors.forename: Xu
authors.initials: X
authors.lastname: Zhu
authors.forename: Hong
authors.initials: H
authors.lastname: Jiang
authors.forename: Qishen
authors.initials: Q
authors.lastname: Cao
authors.forename: Xuying
authors.initials: X
authors.lastname: Li
authors.forename: Fanhao
authors.initials: F
authors.lastname: Meng
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000097:blood / Q000302:isolation & purification / Q000493:pharmacokinetics; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000097:blood / Q000302:isolation & purification / Q000493:pharmacokinetics; D000975:Antioxidants / Q000008:administration & dosage / Q000032:analysis* / Q000302:isolation & purification / Q000493:pharmacokinetics*; D047310:Apigenin / Q000008:administration & dosage / Q000097:blood* / Q000302:isolation & purification / Q000493:pharmacokinetics*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D004355:Drug Stability; D005960:Glucosides / Q000008:administration & dosage / Q000097:blood* / Q000302:isolation & purification / Q000493:pharmacokinetics*; D006207:Half-Life; D027582:Humulus / Q000737:chemistry; D007275:Injections, Intravenous; D057230:Limit of Detection; D008657:Metabolic Clearance Rate; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D005960:Glucosides; C057792:apigetrin; D047310:Apigenin