title: Patterned hydrogel substrates for cell culture with electrohydrodynamic jet printing.
abstract: Cells respond to and are directed by physiochemical cues in their microenvironment, including geometry and substrate stiffness. The development of substrates for cell culture with precisely controlled physiochemical characteristics has the potential to advance the understanding of cell biology considerably. In this communication, E-jet printing is introduced as a method for creating high-resolution protein patterns on substrates with controlled elasticity. It is the first application of E-jet printing on a soft surface. Protein spots as small as 5 µm in diameter on polyacrylamide are demonstrated. The patterned hydrogels are shown to support cell attachment and spreading. Polyacrylamide substrates patterned by E-jet printing may be applied to further the study of cellular mechanobiology.
authors.affiliation: Department of Bioengineering, University of Illinois at Urbana-Champaign, 1304 W. Springfield Ave., Urbana, IL 61801, USA.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Poellmann
authors.forename: Kira L
authors.initials: KL
authors.lastname: Barton
authors.forename: Sandipan
authors.initials: S
authors.lastname: Mishra
authors.forename: Amy J Wagoner
authors.initials: AJ
authors.lastname: Johnson
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D015394:Molecular Structure; D011327:Printing / Q000379:methods*; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry; D013499:Surface Properties; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011506:Proteins; D000099166:Polyacrylamides