title: Suitability of non-lethal marker and marker-free systems for development of transgenic crop plants: present status and future prospects.
abstract: Genetically modified crops are one of the prudent options for enhancing the production and productivity of crop plants by safeguarding from the losses due to biotic and abiotic stresses. Agrobacterium-mediated and biolistic transformation methods are used to develop transgenic crop plants in which selectable marker genes (SMG) are generally deployed to identify 'true' transformants. The commonly used SMG obtained from prokaryotic sources when employed in transgenic plants pose risks due to their lethal nature during selection process. In the recent past, some non-lethal SMGs have been identified and used for selection of transformants with increased precision and high selection efficiency. Considering the concerns related to bio-safety of the environment, it is desirable to remove the SMG in order to maximize the commercial success through wide adoption and public acceptance of genetically modified (GM) food crops. In this review, we examine the availability, and the suitability of wide range of non-lethal selection markers and elimination of SMG methods to develop marker-free transgenics for achieving global food security. As the strategies for marker-free plants are still in proof-of-concept stage, adaptation of new genomics tools for identification of novel non-lethal marker systems and its application for developing marker-free transgenics would further strengthen the crop improvement program.
authors.affiliation: Biotechnology Laboratory, Crop Improvement Section, Directorate of Rice Research, Rajendranagar, Hyderabad 500030, India.
authors.forename: P
authors.initials: P
authors.lastname: Manimaran
authors.forename: G
authors.initials: G
authors.lastname: Ramkumar
authors.forename: K
authors.initials: K
authors.lastname: Sakthivel
authors.forename: R M
authors.initials: RM
authors.lastname: Sundaram
authors.forename: M S
authors.initials: MS
authors.lastname: Madhav
authors.forename: S M
authors.initials: SM
authors.lastname: Balachandran
mesh_terms: D060054:Agrobacterium; D017930:Genes, Reporter; D005818:Genetic Engineering; D005819:Genetic Markers; D030821:Plants, Genetically Modified; D014170:Transformation, Genetic; D027843:Vitis
chemical_list: D005819:Genetic Markers