title: Oligodendrocyte vulnerability following traumatic brain injury in rats.
abstract: Experimental and clinical findings demonstrate that traumatic brain injury (TBI) results in injury to both gray and white matter structures. The purpose of this study was to document patterns of oligodendrocyte vulnerability to TBI. Sprague Dawley rats underwent sham operated procedures or moderate fluid percussion brain injury. Quantitative immunohistochemical analysis was performed on animals perfusion-fixed at 3 (n=9) or 7 (n=9) days post-surgery. Within the ipsilateral external capsule and corpus callosum, numbers of APC-CC1 immunoreactive oligodendrocytes were significantly decreased at 3 or 7 days post-TBI compared to sham rats (p<0.03). At both posttraumatic survival periods, double-labeling studies indicated that oligodendrocytes showed increased Caspase 3 activation compared to sham. These data demonstrate regional patterns of oligodendrocyte vulnerability after TBI and that oligodendrocyte cell loss may be due to Caspase 3-mediated cell death mechanisms. Further studies are needed to test therapeutic interventions that prevent trauma-induced oligodendrocyte cell death, subsequent demyelination and circuit dysfunction.
authors.affiliation: The Miami Project to Cure Paralysis, University of Miami Miller School of Medicine, Miami, FL, USA.
authors.forename: George
authors.initials: G
authors.lastname: Lotocki
authors.forename: Juan Pablo
authors.initials: JP
authors.lastname: de Rivero Vaccari
authors.forename: Juan
authors.initials: J
authors.lastname: de Rivero Vaccari
authors.forename: Ofelia
authors.initials: O
authors.lastname: Alonso
authors.forename: Juliana Sanchez
authors.initials: JS
authors.lastname: Molano
authors.forename: Ryan
authors.initials: R
authors.lastname: Nixon
authors.forename: Padideh
authors.initials: P
authors.lastname: Safavi
authors.forename: W Dalton
authors.initials: WD
authors.lastname: Dietrich
authors.forename: Helen M
authors.initials: HM
authors.lastname: Bramlett
mesh_terms: D025601:Adenomatous Polyposis Coli Protein / Q000378:metabolism; D000818:Animals; D001930:Brain Injuries / Q000378:metabolism / Q000473:pathology*; D053148:Caspase 3 / Q000378:metabolism; D016923:Cell Death; D008297:Male; D009836:Oligodendroglia / Q000378:metabolism / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D025601:Adenomatous Polyposis Coli Protein; D053148:Caspase 3