title: Characterization of α/β- and γ-gliadins in commercial varieties and breeding lines of durum wheat using MALDI-TOF and A-PAGE gels.
abstract: In this work, gliadin composition has been analyzed in 33 accessions of durum wheat using MALDI-TOF MS and compared with A-PAGE results. The MALDI-TOF MS spectra were 29,900-42,500 Da, which corresponds to the α/β- and γ-gliadin regions in A-PAGE. The average of gliadin peaks per line was 23 for MALDI-TOF MS and only 14.8 bands for A-PAGE. MALDI-TOF MS identified 33 gliadin peaks in the durum wheat collection, 20 of which were unique peaks present in 7 lines. A-PAGE analysis identified 30 bands, of which only 4 were unique. Thus, the MALDI-TOF MS method was more sensitive than A-PAGE for identifying α/β- and γ-gliadins in the 33 durum wheat lines studied. Phylogenetic analyses performed using MALDI-TOF MS data assigned the durum wheat lines to two groups. The utility of MALDI-TOF MS to determine relationships among genotypes and for identification of durum wheat accessions is discussed.
authors.affiliation: Departamento de Mejora Genética Vegetal, Instituto de Agricultura Sostenible, 14080 Córdoba, Spain.
authors.forename: Santiago
authors.initials: S
authors.lastname: Marín
authors.forename: Javier
authors.initials: J
authors.lastname: Gil-Humanes
authors.forename: Alberto
authors.initials: A
authors.lastname: Hernando
authors.forename: Francisco
authors.initials: F
authors.lastname: Barro
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel; D005903:Gliadin / Q000737:chemistry* / Q000235:genetics*; D010802:Phylogeny; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014908:Triticum / Q000145:classification / Q000235:genetics*
chemical_list: D005903:Gliadin