title: Cisplatin induces production of reactive oxygen species via NADPH oxidase activation in human prostate cancer cells.
abstract: This study aimed to examine the roles of reactive oxygen species (ROS) in cisplatin treatment of human prostate cancer cells; hormone-sensitive LNCaP and hormone-refractory PC3 and DU145 cells. Intracellular levels of ROS and H(2)O(2) were measured and visualized using specific fluorescent probes. NADPH oxidase (NOX) activity was detected by lucigenin chemiluminescence assay. Expression levels of NOX isoforms were determined by semi-quantitative RT-PCR. Cisplatin treatment increased the intracellular levels of ROS and H(2)O(2) in three prostate cancer cell lines. The increase was transient and robust in hormone-sensitive LNCaP cells compared with hormone-refractory PC3 and DU145 cells. Consistent with these findings, the NOX activity induced by cisplatin was higher in LNCaP cells than in PC3 and DU145 cells. Expression pattern of NOX isoforms varied among three cell lines and the NOX activity was independent of NOX expression. Taken together, we have shown that cisplatin induces production of ROS and H(2)O(2) via NOX activation in human prostate cancer cell lines, which is most prominent in hormone-sensitive LNCaP cells.
authors.affiliation: Faculty of Agriculture, Kinki University, Nakamachi, Nara, Japan. titoh@giib.or.jp
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Itoh
authors.forename: Riyako
authors.initials: R
authors.lastname: Terazawa
authors.forename: Keitaro
authors.initials: K
authors.lastname: Kojima
authors.forename: Keita
authors.initials: K
authors.lastname: Nakane
authors.forename: Takashi
authors.initials: T
authors.lastname: Deguchi
authors.forename: Masashi
authors.initials: M
authors.lastname: Ando
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Tsukamasa
authors.forename: Masafumi
authors.initials: M
authors.lastname: Ito
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Nozawa
mesh_terms: D017738:Alkanesulfonic Acids / Q000737:chemistry; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002166:Camptothecin / Q000494:pharmacology; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis / Q000378:metabolism; D008297:Male; D053078:Membrane Potential, Mitochondrial / Q000502:physiology; D019255:NADPH Oxidases / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000201:enzymology*; D017382:Reactive Oxygen Species / Q000032:analysis / Q000378:metabolism*
chemical_list: D017738:Alkanesulfonic Acids; D000970:Antineoplastic Agents; D017382:Reactive Oxygen Species; C012210:BES; D006861:Hydrogen Peroxide; D019255:NADPH Oxidases; D002945:Cisplatin; D002166:Camptothecin