title: Detection of antigenic proteins expressed by lymphocystis virus as vaccine candidates in olive flounder, Paralichthys olivaceus (Temminck & Schlegel).
abstract: Although the major capsid proteins (MCPs) of lymphocystis disease virus (LCDV) have been characterized, little is known about the host-derived immune response to MCPs and other LCDV antigenic proteins. To identify antigenic proteins of LCDV that could be used as vaccine candidates in olive flounder, Paralichthys olivaceus, we analysed the viral proteins responsible for its virulence by applying immuno-proteomics. LCDV proteins were separated by one-dimensional gel electrophoresis, transferred to polyvinylidene difluoride membrane, and probed with homogeneous P. olivaceus antisera elicited by LCDV natural infection and vaccination with formalin-killed LCDV. Four immune-reactive proteins were obtained at 68-, 51-, 41- and 21 kDa using antisera collected from natural infection while two proteins at 51- and 21 kDa exhibited response to antisera from vaccinated fish, indicating that the latter two proteins have vaccine potential. Using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and nanoelectrospray MS/MS, the 51 and 21 kDa proteins were identified as MCP and an unknown protein, respectively.
authors.affiliation: Aquatic Biotechnology Center of WCU Project, College of Veterinary Medicine, Gyeongsang National University, Jinju, Korea.
authors.forename: H B
authors.initials: HB
authors.lastname: Jang
authors.forename: Y R
authors.initials: YR
authors.lastname: Kim
authors.forename: I S
authors.initials: IS
authors.lastname: Cha
authors.forename: S W
authors.initials: SW
authors.lastname: Noh
authors.forename: S B
authors.initials: SB
authors.lastname: Park
authors.forename: M
authors.initials: M
authors.lastname: Ohtani
authors.forename: J
authors.initials: J
authors.lastname: Hikima
authors.forename: T
authors.initials: T
authors.lastname: Aoki
authors.forename: T S
authors.initials: TS
authors.lastname: Jung
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis* / Q000302:isolation & purification; D015153:Blotting, Western / Q000662:veterinary; D036022:Capsid Proteins / Q000032:analysis / Q000302:isolation & purification; D004266:DNA Virus Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary* / Q000821:virology; D004586:Electrophoresis / Q000662:veterinary; D005393:Fish Diseases / Q000276:immunology / Q000517:prevention & control / Q000821:virology*; D005432:Flounder / Q000821:virology*; D007497:Iridoviridae / Q000276:immunology*; D008969:Molecular Sequence Data; D020543:Proteome / Q000032:analysis* / Q000302:isolation & purification; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000662:veterinary; D053719:Tandem Mass Spectrometry / Q000662:veterinary; D014611:Vaccination / Q000662:veterinary; D014612:Vaccines / Q000276:immunology*
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; D020543:Proteome; D014612:Vaccines