title: 'Candidatus Phytoplasma sudamericanum', a novel taxon, and strain PassWB-Br4, a new subgroup 16SrIII-V phytoplasma, from diseased passion fruit (Passiflora edulis f. flavicarpa Deg.).
abstract: Symptoms of abnormal proliferation of shoots resulting in formation of witches'-broom growths were observed on diseased plants of passion fruit (Passiflora edulis f. flavicarpa Deg.) in Brazil. RFLP analysis of 16S rRNA gene sequences amplified in PCRs containing template DNAs extracted from diseased plants collected in Bonito (Pernambuco) and Viçosa (Minas Gerais) Brazil, indicated that such symptoms were associated with infections by two mutually distinct phytoplasmas. One phytoplasma, PassWB-Br4 from Bonito, represents a new subgroup, 16SrIII-V, in the X-disease phytoplasma group ('Candidatus Phytoplasma pruni'-related strains). The second phytoplasma, PassWB-Br3 from Viçosa, represents a previously undescribed subgroup in group 16SrVI. Phylogenetic analyses of 16S rRNA gene sequences were consistent with the hypothesis that strain PassWB-Br3 is distinct from previously described 'Ca. Phytoplasma' species. Nucleotide sequence alignments revealed that strain PassWB-Br3 shared less than 97.5 % 16S rRNA gene sequence similarity with previously described 'Ca. Phytoplasma' species. The unique properties of its DNA, in addition to natural host and geographical occurrence, support the recognition of strain PassWB-Br3 as a representative of a novel taxon, 'Candidatus Phytoplasma sudamericanum'.
authors.affiliation: Molecular Plant Pathology Laboratory, USDA-Agricultural Research Service, Beltsville, MD 20705, USA.
authors.forename: Robert E
authors.initials: RE
authors.lastname: Davis
authors.affiliation: Molecular Plant Pathology Laboratory, USDA-Agricultural Research Service, Beltsville, MD 20705, USA.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Molecular Plant Pathology Laboratory, USDA-Agricultural Research Service, Beltsville, MD 20705, USA.
authors.forename: Ellen L
authors.initials: EL
authors.lastname: Dally
authors.affiliation: Phytovirus Laboratory, Nature Research Centre, Vilnius, Lithuania.|Molecular Plant Pathology Laboratory, USDA-Agricultural Research Service, Beltsville, MD 20705, USA.
authors.forename: Rasa
authors.initials: R
authors.lastname: Jomantiene
authors.affiliation: Molecular Plant Pathology Laboratory, USDA-Agricultural Research Service, Beltsville, MD 20705, USA.
authors.forename: Ing-Ming
authors.initials: IM
authors.lastname: Lee
authors.affiliation: University of Maryland, Biotechnology Institute, Center for Biosystems Research, Rockville, MD 20850, USA.|Molecular Plant Pathology Laboratory, USDA-Agricultural Research Service, Beltsville, MD 20705, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.affiliation: Departamento de Fitopatologia e Nematologia, Escola Superior de Agricultura 'Luiz de Queiroz', Universidade de São Paulo, 13418-900 Piracicaba, Sao Paolo, Brazil.
authors.forename: Elliot W
authors.initials: EW
authors.lastname: Kitajima
mesh_terms: D001938:Brazil; D008969:Molecular Sequence Data; D029598:Passiflora / Q000382:microbiology*; D010802:Phylogeny; D045582:Phytoplasma / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010935:Plant Diseases / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D016415:Sequence Alignment
chemical_list: D012336:RNA, Ribosomal, 16S