title: Single-step conjugation of antibodies to quantum dots for labeling cell surface receptors in mammalian cells.
abstract: Labeling of cell surface receptors in living cells can be achieved using antibody-conjugated semiconductor quantum dots (QDs). The inherent photostable property of QDs can be exploited for understanding the arrangement and distribution of receptors in the plasma membrane. We describe herein methods that allow conjugation of antibodies to QDs in a single step without the formation of side products. This protocol can be adapted universally for any type of QD structure with a coating of free amino groups.
authors.forename: Gopal
authors.initials: G
authors.lastname: Iyer
authors.forename: Jianmin
authors.initials: J
authors.lastname: Xu
authors.forename: Shimon
authors.initials: S
authors.lastname: Weiss
mesh_terms: D000588:Amines / Q000737:chemistry; D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000737:chemistry* / Q000276:immunology; D001547:Benzaldehydes / Q000737:chemistry; D045744:Cell Line, Tumor; D002470:Cell Survival; D066246:ErbB Receptors / Q000737:chemistry* / Q000276:immunology; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008856:Microscopy, Fluorescence; D057054:Molecular Imaging; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry; D045663:Quantum Dots; D013194:Staining and Labeling / Q000379:methods*; D013388:Succinimides / Q000737:chemistry
chemical_list: D000588:Amines; D000906:Antibodies; D001547:Benzaldehydes; D010455:Peptides; D013388:Succinimides; D066246:ErbB Receptors; C001426:N-hydroxysuccinimide