title: Cell biology of homologous recombination in yeast.
abstract: Homologous recombination is an important pathway for error-free repair of DNA lesions, such as single- and double-strand breaks, and for rescue of collapsed replication forks. Here, we describe protocols for live cell imaging of single-lesion recombination events in the yeast Saccharomyces cerevisiae using fluorescence microscopy.
authors.affiliation: Department of Biology, University of Copenhagen, DK-2200, Copenhagen, Denmark. nadine@bio.ku.dk
authors.forename: Nadine
authors.initials: N
authors.lastname: Eckert-Boulet
authors.forename: Rodney
authors.initials: R
authors.lastname: Rothstein
authors.forename: Michael
authors.initials: M
authors.lastname: Lisby
mesh_terms: D053903:DNA Breaks, Double-Stranded; D004249:DNA Damage / Q000235:genetics; D008856:Microscopy, Fluorescence; D011995:Recombination, Genetic / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics