title: Comparison of two molecular methods for rapid diagnosis of extrapulmonary tuberculosis.
abstract: Application of real-time PCR for the detection of Mycobacterium tuberculosis enables results to be obtained in about 2 h. A total of 340 nonrespiratory samples were processed using two real-time PCR assay kits: Xpert MTB/RIF and Cobas TaqMan MTB. The sensitivity and specificity of the Xpert assay were 95% and 100%, respectively, compared to 78% and 98% for the Cobas assay.
authors.affiliation: Microbiology Service, Reina Sofia University Hospital, Mycobacteria Reference Center, Avda. Menendez Pidal s/n, 14004 Córdoba, Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: Causse
authors.forename: Pilar
authors.initials: P
authors.lastname: Ruiz
authors.forename: Juan Bautista
authors.initials: JB
authors.lastname: Gutiérrez-Aroca
authors.forename: Manuel
authors.initials: M
authors.lastname: Casal
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001431:Bacteriological Techniques / Q000379:methods*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D014376:Tuberculosis / Q000175:diagnosis*; D055815:Young Adult