title: Rapid identification of Cryptococcus neoformans and Cryptococcus gattii by matrix-assisted laser desorption ionization-time of flight mass spectrometry.
abstract: Compared to DNA sequence analysis, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) correctly identified 100% of Cryptococcus species, distinguishing the notable pathogens Cryptococcus neoformans and C. gattii. Identification was greatly enhanced by supplementing a commercial spectral library with additional entries to account for subspecies variability.
authors.affiliation: Mycology Department, Ontario Agency for Health Protection and Promotion, 81 Resources Rd., Toronto, Ontario M9P 3T1, Canada. lisa.mctaggart@oahpp.ca
authors.forename: Lisa R
authors.initials: LR
authors.lastname: McTaggart
authors.forename: Eric
authors.initials: E
authors.lastname: Lei
authors.forename: Susan E
authors.initials: SE
authors.lastname: Richardson
authors.forename: Linda
authors.initials: L
authors.lastname: Hoang
authors.forename: Annette
authors.initials: A
authors.lastname: Fothergill
authors.forename: Sean X
authors.initials: SX
authors.lastname: Zhang
mesh_terms: D056285:Cryptococcus gattii / Q000737:chemistry* / Q000145:classification*; D003455:Cryptococcus neoformans / Q000737:chemistry* / Q000145:classification*; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D006801:Humans; D008828:Microbiological Techniques / Q000379:methods*; D009172:Mycology / Q000379:methods*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D004271:DNA, Fungal