title: Simple colorimetric sensing of trace bleomycin using unmodified gold nanoparticles.
abstract: A simple colorimetric sensing platform for trace bleomycin (BLM) was proposed with the unmodified gold nanoparticles (AuNPs) as the sensing element. BLM has multiple N-donor functionality and exhibited strong coordination effect on AuNPs, which made it possible for the occurrence of ligand exchange of BLM with the weakly surface-bound citrate ions on AuNPs. Meanwhile, the positively charged BLM molecules further neutralized the surface charge, leading to increased van der Waals attractive force among AuNPs for rapid aggregation. This was reflected by the obvious color change from wine red to blue and rapid aggregation kinetics within 7.5 min. The BLM sensing based on unmodified AuNPs can be seen with the naked eye and monitored by UV-vis extinction spectra. The linear range of the colorimetric sensor for BLM was from 2 to 150 nM. The as-established colorimetric strategy opened a new avenue for trace BLM determination.
authors.affiliation: College of Chemistry, Chemical Engineering and Materials Science, Engineering Research Center of Pesticide and Medicine Intermediate Clean Production, Ministry of Education, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Normal University, Jinan 250014, People's Republic of China.
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Feng
authors.forename: Can
authors.initials: C
authors.lastname: Zhao
authors.forename: Bo
authors.initials: B
authors.lastname: Tang
mesh_terms: D000970:Antineoplastic Agents / Q000032:analysis / Q000097:blood; D015374:Biosensing Techniques / Q000379:methods*; D001761:Bleomycin / Q000032:analysis* / Q000097:blood; D003124:Colorimetry / Q000379:methods*; D006046:Gold; D006801:Humans; D053768:Metal Nanoparticles / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D013053:Spectrophotometry
chemical_list: D000970:Antineoplastic Agents; D001761:Bleomycin; D006046:Gold