title: Lindnera (Pichia) fabianii blood infection after mesenteric ischemia.
abstract: Lindnera (Pichia) fabianii (teleomorph of Candida fabianii) is a yeast species rarely involved in human infections. This report describes the first known human case of a Lindnera fabianii blood infection after mesenteric ischemia. The 53-year-old patient was hospitalized in the intensive care unit after a suicide attempt and was suffering from a mesenteric ischemia and acute renal failure. Lindnera fabianii was recovered from an oropharyngeal swab, then isolated from stool and urine samples before the diagnosis of the blood infection. Caspofungin intravenous treatment was associated with a successful outcome. Final unequivocal identification of the strain was done by sequencing the internal transcribed spacer (ITS) region, and regions of 18S rDNA gene and of the translation elongation factor-1α gene. Until our work, the genomic databases did not contain the complete ITS region of L. fabianii as a single nucleotide sequence (encompassing ITS1, the 5.8S rDNA and ITS2), and misidentification with other yeast species, e.g., Lindnera (Pichia) mississippiensis, could have occurred. Our work demonstrates that the usual DNA barcoding method based on sequencing of the ITS region may fail to provide the correct identification of some taxa, and that partial sequencing of the EF1α gene may be much more effective for the accurate delineation and molecular identification of new emerging opportunistic yeast pathogens.
authors.affiliation: Université de Bordeaux, Bordeaux, France.
authors.forename: Frederic
authors.initials: F
authors.lastname: Gabriel
authors.forename: Thierry
authors.initials: T
authors.lastname: Noel
authors.forename: Isabelle
authors.initials: I
authors.lastname: Accoceberry
mesh_terms: D058186:Acute Kidney Injury / Q000150:complications; D000935:Antifungal Agents / Q000008:administration & dosage; D001769:Blood / Q000382:microbiology; D000077336:Caspofungin; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D054714:Echinocandins / Q000008:administration & dosage; D005260:Female; D016469:Fungemia / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology; D020459:Genes, rRNA; D006801:Humans; D007511:Ischemia / Q000150:complications*; D055666:Lipopeptides; D065666:Mesenteric Ischemia; D008875:Middle Aged; D012331:RNA, Fungal / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D004718:Saccharomycetales / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA; D013405:Suicide; D016896:Treatment Outcome; D014652:Vascular Diseases / Q000150:complications*
chemical_list: D000935:Antifungal Agents; D004271:DNA, Fungal; D004275:DNA, Ribosomal; D054714:Echinocandins; D055666:Lipopeptides; D012331:RNA, Fungal; D012337:RNA, Ribosomal, 18S; D000077336:Caspofungin