title: Antihaemorrhagic potentials of Fagonia cretica against Naja naja karachiensis (black Pakistan cobra) venom.
abstract: Plants have been extensively used as a remedy for the treatment of snake bites. The aim of this study was to determine the antivenom potentials of methanolic extract from the aerial parts (leaves and twigs) of Fagonia cretica L. on a haemorrhage induced by venom from Naja naja karachiensis. The haemorrhagic response of venom was dose dependent from 0.1 to 4.0 µg per 1.5 µL phosphate buffer saline (PBS) on vitelline veins of fertilised hens' eggs in their shells. The extract effectively eliminated and neutralised, in a dose-dependent manner, the haemorrhagic activity of snake venom. The minimum effective neutralising dose of F. cretica extract was found to be 15 µg per 1.5 µL PBS. The extract possesses potentials as haemorrhagic inhibitor against snake venom compared to the standard antiserum and various plants reported in the literature. This study also provides a scientific base for the use of F. cretica in traditional medicine for the treatment of snake bite.
authors.affiliation: Faculty of Pharmacy, Bahauddin-Zakariya-University, Multan, Pakistan.
authors.forename: Muhammad Tahir
authors.initials: MT
authors.lastname: Razi
authors.forename: Muhammad Hassham Hassan Bin
authors.initials: MH
authors.lastname: Asad
authors.forename: Taous
authors.initials: T
authors.lastname: Khan
authors.forename: Muhammad Zabta
authors.initials: MZ
authors.lastname: Chaudhary
authors.forename: Muhammad Tayyab
authors.initials: MT
authors.lastname: Ansari
authors.forename: Muhammad Anwar
authors.initials: MA
authors.lastname: Arshad
authors.forename: Qazi Najam-us
authors.initials: QN
authors.lastname: Saqib
mesh_terms: D000818:Animals; D000997:Antivenins / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D002642:Chick Embryo; D004305:Dose-Response Relationship, Drug; D004546:Elapid Venoms / Q000633:toxicity*; D017815:Elapidae; D006470:Hemorrhage / Q000188:drug therapy* / Q000209:etiology / Q000517:prevention & control; D010154:Pakistan; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D018515:Plant Leaves / Q000737:chemistry*; D028461:Zygophyllaceae / Q000737:chemistry*
chemical_list: D000997:Antivenins; D004546:Elapid Venoms; D010936:Plant Extracts