title: Outcome of cycles of oocyte in vitro maturation requiring testicular sperm extraction for nonobstructive azoospermia.
abstract: OBJECTIVE
  To assess the applicability of oocyte IVM in case of nonobstructive azoospermia (NOA).
  
  
  METHODS
  Case series.
  
  
  METHODS
  Private IVF unit.
  
  
  METHODS
  All male partners were affected by NOA. Twenty-one women (20 normo-ovulatory and 1 with polycystic ovary) underwent 27 IVM treatments. The outcome of controlled ovarian stimulation (COS) cycles in normo-ovulatory women whose partners were affected by NOA was assessed for comparison.
  
  
  METHODS
  Spermatozoa from testicular sperm extraction (TESE) samples were retrieved and cryopreserved before treatment.
  
  
  METHODS
  Rates of fertilization, pregnancy per cycle and transfer, and implantation.
  
  
  RESULTS
  Twenty-seven IVM-TESE cycles were carried out in 21 women. Fertilization rate was 64.4%. In compliance with national legislation, no more than three oocytes were inseminated in each cycle and all developing embryos were transferred. Six single clinical pregnancies were ascertained (22.2% and 27.3% pregnancy rates per cycle and per transfer, respectively). One pregnancy ended in abortion. The others developed to term and gave rise to five healthy babies. Sixty couples underwent standard COS-TESE treatment. Rates of fertilization, clinical pregnancy per cycle, implantation, and abortion were 64.6%, 20.0%, 11.3%, and 16.7%, respectively. Eleven babies were born from ten pregnancies.
  
  
  CONCLUSIONS
  Oocyte IVM may represent an option in NOA cases.
authors.affiliation: Biogenesi, Reproductive Medicine Center, Istituti Clinici Zucchi, Monza, Italy. rfadini@fadini.it
authors.forename: Rubens
authors.initials: R
authors.lastname: Fadini
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Colpi
authors.forename: Mario
authors.initials: M
authors.lastname: Mignini Renzini
authors.forename: Giovanni
authors.initials: G
authors.lastname: Coticchio
authors.forename: Ruggero
authors.initials: R
authors.lastname: Comi
authors.forename: Massimo
authors.initials: M
authors.lastname: Mastrolilli
authors.forename: Teresa
authors.initials: T
authors.lastname: Guarnieri
authors.forename: Annalisa
authors.initials: A
authors.lastname: Villa
authors.forename: Mariabeatrice
authors.initials: M
authors.lastname: Dal Canto
mesh_terms: D000022:Abortion, Spontaneous / Q000209:etiology; D000328:Adult; D053713:Azoospermia / Q000628:therapy*; D002478:Cells, Cultured; D015925:Cryopreservation; D046149:Embryo Culture Techniques; D004624:Embryo Transfer; D005260:Female; D006801:Humans; D007558:Italy; D050498:Live Birth; D008297:Male; D054315:Oocyte Retrieval / Q000009:adverse effects; D009865:Oocytes / Q000502:physiology*; D011247:Pregnancy; D018873:Pregnancy Rate; D012662:Semen Preservation; D020554:Sperm Injections, Intracytoplasmic / Q000009:adverse effects; D053625:Sperm Retrieval / Q000009:adverse effects; D016896:Treatment Outcome