title: Development of a multi-mycotoxin analysis in beer-based drinks by a modified QuEChERS method and ultra-high-performance liquid chromatography coupled with tandem mass spectrometry.
abstract: An analytical method was developed for the identification and quantification of 15 mycotoxins (patulin, nivalenol, deoxynivalenol, aflatoxin B(1), B(2), G(1), G(2), M(1), T-2 toxin, HT-2 toxin, zearalenone, fumonisin B(1), B(2), B(3), and ochratoxin A) in beer-based drinks (beer, low-malt beer, new genre, and nonalcoholic) by a modified QuEChERS method and an ultra-high-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC/MS/MS). Mycotoxins were extracted from samples using acetonitrile with sodium chloride, anhydrous magnesium sulfate, and sodium citrate, and were then purified with a solid phase extraction (SPE) cartridge including C18. The UHPLC conditions were also examined to establish its optimal conditions for separation. Fifteen mycotoxins were separated in a total of 6.5 min, and were quantified in the optimal mobile phase conditions. Determinations performed using this method produced high correlation coefficients of 15 mycotoxins (R>0.99) and recovery rates ranging from 70.3 to 110.7% with good repeatability (relative standard deviation RSD<14.6%). Further, 24 commercial beer-based drinks in Japan were analyzed using this method, and nivalenol, deoxynivalenol, and fumonisins were detected in several samples, but always under the limit of quantification (<5 ng/mL). These results suggest that the health risk to consumers from beer-based drinks in Japan is relatively low.
authors.affiliation: Research Laboratories for Food Safety Chemistry, Asahi Breweries, Limited, 1-1-21 Midori, Moriya, Ibaraki 302-0106, Japan.
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Tamura
authors.forename: Atsuo
authors.initials: A
authors.lastname: Uyama
authors.forename: Naoki
authors.initials: N
authors.lastname: Mochizuki
mesh_terms: D001515:Beer / Q000032:analysis*; D001628:Beverages / Q000032:analysis*; D005591:Chemical Fractionation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D015394:Molecular Structure; D009183:Mycotoxins / Q000032:analysis*; D053719:Tandem Mass Spectrometry
chemical_list: D009183:Mycotoxins