title: Chemical constituents from Lobelia chinensis and their anti-virus and anti-inflammatory bioactivities.
abstract: In total, forty six compounds, including the novel compound lobechine (1), were characterized from the methanol extracts of Lobelia chinensis. The chemical structures of known metabolites were identified by comparing their spectroscopic and physical data with compounds reported in the literature. The structure of lobechine (1) was comprehensively established with the aid of 1D and 2D NMR spectroscopic analyses. In addition, selected isolates were screened for their inhibition of HSV-1 replication, superoxide anion generation, and elastase release. Among the tested compounds, scoparone (10) exhibited significant inhibition of superoxide anion generation with IC(50) of 6.14 ± 1.97 μM and lobechine (1) exhibited moderate inhibition of elastase release with IC(50) of 25.01 ± 6.95 μM, respectively.
authors.affiliation: Department of Biotechnology, National Formosa University, Yunlin 632, Taiwan.
authors.forename: Ping-Chung
authors.initials: PC
authors.lastname: Kuo
authors.forename: Tsong-Long
authors.initials: TL
authors.lastname: Hwang
authors.forename: Ying-Ting
authors.initials: YT
authors.lastname: Lin
authors.forename: Yuh-Chi
authors.initials: YC
authors.lastname: Kuo
authors.forename: Yann-Lii
authors.initials: YL
authors.lastname: Leu
mesh_terms: D000328:Adult; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000998:Antiviral Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002087:Butyrates / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D015550:Cell Degranulation / Q000187:drug effects; D003374:Coumarins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D006367:HeLa Cells; D018259:Herpesvirus 1, Human / Q000187:drug effects / Q000254:growth & development / Q000502:physiology; D006801:Humans; D007536:Isomerism; D019272:Leukocyte Elastase / Q000378:metabolism; D029746:Lobelia / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D015394:Molecular Structure; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D011758:Pyrroles / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D013481:Superoxides / Q000378:metabolism; D010948:Viral Plaque Assay; D014779:Virus Replication / Q000187:drug effects; D055815:Young Adult
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D000998:Antiviral Agents; D002087:Butyrates; D003374:Coumarins; D004365:Drugs, Chinese Herbal; D011758:Pyrroles; C561629:lobechine; D013481:Superoxides; D019272:Leukocyte Elastase; C018145:scoparone