title: Interplay of early biochemical manifestations by cadmium insult in sertoli-germ coculture: an in vitro study.
abstract: Cadmium is a common environmental and occupational hazard and its adverse effect on reproductive organ has been well documented. The present study is planned to delineate the mechanism of Cd toxicity in rat testes. Our study shows that Cd causes apoptosis in sertoli-germ cells which is governed by oxidative stress. We assayed ROS, GSH and MMP to ensure the role of oxidative stress, further confirmed it by thiol modulators. The initial biochemical response shown in sertoli-germ cells was a significant rise in intracellular calcium followed by a drastic fall in MMP and then ROS generation. The downstream events included cytochrome c release leading to caspase-3 activation and culminating in cell death via apoptosis. Furthermore Cd disrupted the spermatogenic pathway as evident by suppression in tesmin and LDH-X levels.
authors.affiliation: Immunotoxicology Lab, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), Lucknow, India.
authors.forename: Smita
authors.initials: S
authors.lastname: Khanna
authors.forename: Pramesh C
authors.initials: PC
authors.lastname: Lakhera
authors.forename: Shashi
authors.initials: S
authors.lastname: Khandelwal
mesh_terms: D000818:Animals; D002104:Cadmium / Q000633:toxicity*; D053148:Caspase 3 / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D018920:Coculture Techniques; D045304:Cytochromes c / Q000378:metabolism; D004249:DNA Damage; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D012708:Sertoli Cells / Q000187:drug effects* / Q000378:metabolism; D013094:Spermatozoa / Q000187:drug effects* / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D002104:Cadmium; D045304:Cytochromes c; D053148:Caspase 3