title: Influenza A virus N5 neuraminidase has an extended 150-cavity.
abstract: There are 9 serotypes of neuraminidase (NA) from influenza A virus (N1 to N9), which are classified into two groups based on primary sequences (groups 1 and 2). The structural hallmark of the two groups is the presence or absence of an extra 150-cavity (formed by the 150-loop) in the active site. Thus far, structures of NAs from 6 out of the 9 serotypes have been solved. Here, we solved the N5 structure, the last unknown structure group 1 serotype with a unique Asn147 residue in its 150-loop, demonstrating that it has an extended 150-cavity that closes upon inhibitor binding.
authors.affiliation: CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Chaoyang District, Beijing 100101, The People's Republic of China. gaof@im.ac.cn
authors.forename: Mingyang
authors.initials: M
authors.lastname: Wang
authors.forename: Jianxun
authors.initials: J
authors.lastname: Qi
authors.forename: Yue
authors.initials: Y
authors.lastname: Liu
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Vavricka
authors.forename: Yan
authors.initials: Y
authors.lastname: Wu
authors.forename: Qing
authors.initials: Q
authors.lastname: Li
authors.forename: George F
authors.initials: GF
authors.lastname: Gao
mesh_terms: D000595:Amino Acid Sequence; D000998:Antiviral Agents / Q000378:metabolism; D001665:Binding Sites; D018360:Crystallography, X-Ray; D053124:Influenza A Virus, H5N1 Subtype / Q000737:chemistry / Q000235:genetics; D009980:Influenza A virus / Q000737:chemistry* / Q000235:genetics; D008958:Models, Molecular; D009439:Neuraminidase / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D053243:Zanamivir / Q000378:metabolism
chemical_list: D000998:Antiviral Agents; D009439:Neuraminidase; D053243:Zanamivir