title: Cationic polyacrylamides promote binding of cellulase and amylase.
abstract: Cationic polyacrylamides (c-PAMs) increase the binding of cellulase to cellulose and of α-amylase to cornstarch. The c-PAMs increase the rate of hydrolysis of both substrates by about the same amount, which suggests that the increased binding is responsible for the enhanced rates. These results support our proposed mechanism where the c-PAM neutralizes the charge of the substrate through a "patching" mechanism, which reduces the charge repulsion between fiber and enzyme.
authors.affiliation: Institute of Paper Science and Technology, School of Chemical & Biomolecular Engineering, Georgia Institute of Technology, 500 10th Street, NW, Atlanta, GA 30332-0620, United States.
authors.forename: John T
authors.initials: JT
authors.lastname: Reye
authors.forename: Kendra E
authors.initials: KE
authors.lastname: Maxwell
authors.forename: Sujit
authors.initials: S
authors.lastname: Banerjee
mesh_terms: D000180:Acrylic Resins / Q000494:pharmacology*; D000681:Amylases / Q000378:metabolism*; D002480:Cellulase / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects; D013213:Starch / Q000378:metabolism; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D000681:Amylases; D002480:Cellulase; D002482:Cellulose; D013213:Starch; D000099166:Polyacrylamides