title: Diospyros blancoi attenuates asthmatic effects in a mouse model of airway inflammation.
abstract: Asthma is a complex disease linked to various pathophysiological events, including proteinase activity. In this study, we examined whether a Diospyros blancoi methanolic extract (DBE) exerts protective effects on allergic asthma in a murine asthma model. To investigate the specific role of DBE, we employed a murine model of allergic airway inflammation. BALB/c mice sensitized and challenged with ovalbumin (OVA) were orally administered 20 or 40 mg/kg DBE for 3 days during OVA challenge. DBE induced significant suppression of the number of OVA-induced total inflammatory cells, including eosinophils, macrophages, and lymphocytes, in bronchoalveolar lavage fluid (BALF). Moreover, treatment with DBE led to significant decreases in interleukin (IL)-4, IL-5, and eotaxin levels in BALF and OVA-specific immunoglobulin (Ig)E and IgG1 levels in serum. Histological examination of lung tissue revealed marked attenuation of allergen-induced lung eosinophilic inflammation and mucus-producing goblet cells in the airway. Additionally, DBE suppressed matrix metalloproteinase-9 activity and induced heme oxygenase-1 expression. The present findings collectively suggest that DBE exhibits anti-inflammatory activity in an airway inflammation mouse model, supporting its therapeutic potential for the treatment of allergic bronchial asthma.
authors.affiliation: Department of Health, Kongju National University, Kongju, South Korea.
authors.forename: Kyoung-Youl
authors.initials: KY
authors.lastname: Lee
authors.forename: Ju-Young
authors.initials: JY
authors.lastname: Jung
authors.forename: Mee-Young
authors.initials: MY
authors.lastname: Lee
authors.forename: Dayoung
authors.initials: D
authors.lastname: Jung
authors.forename: Eun-Sang
authors.initials: ES
authors.lastname: Cho
authors.forename: Hwa-Young
authors.initials: HY
authors.lastname: Son
mesh_terms: D000818:Animals; D018927:Anti-Asthmatic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology / Q000627:therapeutic use*; D001249:Asthma / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology / Q000276:immunology; D029788:Diospyros; D004195:Disease Models, Animal; D004804:Eosinophils / Q000187:drug effects; D005260:Female; D051547:Heme Oxygenase-1 / Q000378:metabolism; D007073:Immunoglobulin E / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D015847:Interleukin-4 / Q000378:metabolism; D015848:Interleukin-5 / Q000378:metabolism; D008168:Lung / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D008214:Lymphocytes / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D018927:Anti-Asthmatic Agents; D000894:Anti-Inflammatory Agents, Non-Steroidal; D007074:Immunoglobulin G; D015848:Interleukin-5; D010936:Plant Extracts; D015847:Interleukin-4; D007073:Immunoglobulin E; D010047:Ovalbumin; D051547:Heme Oxygenase-1; D020780:Matrix Metalloproteinase 9