title: Interaction of histamine and calcitonin gene-related peptide in the formalin induced pain perception in rats.
abstract: Histamine and calcitonin gene-related peptide (CGRP) contribute to the pain perception. The aim of the present study is to clarify the interaction of histamine and CGRP in the perception of inflammatory pain. The effects of a histamine H1 receptor antagonist (pyrilamine, i.p.), an H2 receptor antagonist (ranitidine, i.p.) and a CGRP antagonist (CGRP 8-37, i.t.) on the formalininduced pain was studied in rats. Pyrilamine and ranitidine produced a dose-dependent antinociceptive response in the first and the second phases of the formalin test. A single administration of pyrilamine (1 mg/kg, i.p.), ranitidine (10 mg/kg, i.p.) or CGRP 8-37 (10 µg/µL, i.t.) had no significant effects on the pain perception in the second phase. A combination of CGRP 8-37 and pyrilamine or ranitidine at these sub-effective doses, however, showed nociceptive response in the second phase. Moreover, a histamine (i.t.)-induced hyperalgesia was completely prevented by treatment with GGRP 8-37 at this dose. Our findings have raised the possibility that the CGRP system has interaction with histamine in the perception of inflammatory pain.
authors.affiliation: Department of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran. jalalizadi2002@yahoo.com
authors.forename: Jalal Izadi
authors.initials: JI
authors.lastname: Mobarakeh
authors.forename: Anahita
authors.initials: A
authors.lastname: Torkaman-Boutorabi
authors.forename: Amir Abbas
authors.initials: AA
authors.lastname: Rahimi
authors.forename: Shahrooz
authors.initials: S
authors.lastname: Ghasri
authors.forename: Reza Mohammad Ali
authors.initials: RM
authors.lastname: Nezhad
authors.forename: Arash
authors.initials: A
authors.lastname: Hamzely
authors.forename: Baharak
authors.initials: B
authors.lastname: Khoshkholgh Sima
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Takahashi
authors.forename: Kazuo
authors.initials: K
authors.lastname: Nunoki
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Yanai
mesh_terms: D000700:Analgesics / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000008:administration & dosage / Q000494:pharmacology*; D000077221:Calcitonin Gene-Related Peptide Receptor Antagonists; D004347:Drug Interactions; D004359:Drug Therapy, Combination; D005557:Formaldehyde / Q000009:adverse effects*; D006632:Histamine / Q000494:pharmacology*; D006634:Histamine H1 Antagonists / Q000008:administration & dosage / Q000494:pharmacology; D006635:Histamine H2 Antagonists / Q000008:administration & dosage / Q000494:pharmacology; D006930:Hyperalgesia / Q000139:chemically induced; D008297:Male; D010146:Pain / Q000139:chemically induced / Q000188:drug therapy; D058748:Pain Perception / Q000187:drug effects*; D010446:Peptide Fragments / Q000008:administration & dosage / Q000494:pharmacology*; D011738:Pyrilamine / Q000008:administration & dosage / Q000494:pharmacology; D011899:Ranitidine / Q000008:administration & dosage / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000700:Analgesics; D000077221:Calcitonin Gene-Related Peptide Receptor Antagonists; D006634:Histamine H1 Antagonists; D006635:Histamine H2 Antagonists; D010446:Peptide Fragments; C058636:calcitonin gene-related peptide (8-37); D005557:Formaldehyde; D006632:Histamine; D011899:Ranitidine; D011738:Pyrilamine; D015740:Calcitonin Gene-Related Peptide