title: Emergence and genetic variability of Anaplasma species in small ruminants and ticks from Central Europe.
abstract: Anaplasmoses are common tick-borne zoonotic bacterial diseases of livestock and free-living ungulates from the genus Anaplasma that are recently emerging in Central Europe. The main aim of this study was to analyze the prevalence and genetic variability of Anaplasma phagocytophilum and Anaplasma ovis in small ruminants and questing ticks from six different sites in Slovakia and the Czech Republic using the PCR of the msp4 gene followed by the sequence analysis. At two farms from southeastern Slovakia, 66.1% small ruminants were infected with A. ovis in contrast to one positive animal from both sites in northern Slovakia. It was represented by two different genotypes. A. phagocytophilum was present in all tested flocks with the infection prevalence ranging from 0.9% to 5.7%. None of the tested questing ticks carried A. ovis. A. phagocytophilum was detected in 1.1% and 7.8% of questing Ixodes ricinus ticks collected around the farms located in southeastern and northern Slovakia, respectively. A. phagocytophilum revealed higher intraspecific diversity than A. ovis.
authors.affiliation: Parasitological Institute, Slovak Academy of Sciences, Košice, Slovak Republic. marketa.derdakova@savba.sk
authors.forename: Markéta
authors.initials: M
authors.lastname: Derdáková
authors.forename: Astéria
authors.initials: A
authors.lastname: Stefančíková
authors.forename: Eva
authors.initials: E
authors.lastname: Spitalská
authors.forename: Veronika
authors.initials: V
authors.lastname: Tarageľová
authors.forename: Tatiana
authors.initials: T
authors.lastname: Košťálová
authors.forename: Gabriela
authors.initials: G
authors.lastname: Hrkľová
authors.forename: Kateřina
authors.initials: K
authors.lastname: Kybicová
authors.forename: Pavel
authors.initials: P
authors.lastname: Schánilec
authors.forename: Viktória
authors.initials: V
authors.lastname: Majláthová
authors.forename: Marián
authors.initials: M
authors.lastname: Várady
authors.forename: Branislav
authors.initials: B
authors.lastname: Peťko
mesh_terms: D000709:Anaplasma / Q000145:classification / Q000235:genetics*; D042323:Anaplasma ovis / Q000235:genetics; D000712:Anaplasmosis / Q000382:microbiology*; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D021821:Communicable Diseases, Emerging / Q000382:microbiology / Q000662:veterinary*; D018153:Czech Republic; D014644:Genetic Variation; D015511:Goat Diseases / Q000382:microbiology*; D006041:Goats; D008565:Membrane Proteins / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology*; D018154:Slovakia; D017282:Tick-Borne Diseases / Q000382:microbiology; D013987:Ticks / Q000382:microbiology*
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins; C085498:Msp4 protein, Anaplasma marginale