title: Mir-34a is upregulated during liver regeneration in rats and is associated with the suppression of hepatocyte proliferation.
abstract: BACKGROUND
  MicroRNAs are a class of small regulatory RNAs that modulate a variety of biological processes, including cellular differentiation, apoptosis, metabolism and proliferation. This study aims to explore the effect of miR-34a in hepatocyte proliferation and its potential role in liver regeneration termination.
  
  
  RESULTS
  MiR-34a was highly induced after partial hepatectomy. Overexpression of miR-34a in BRL-3A cells could significantly inhibit cell proliferation and down-regulate the expression of inhibin βB (INHBB) and Met. In BRL-3A cells, INHBB was identified as a direct target of miR-34a by luciferase reporter assay. More importantly, INHBB siRNA significantly repressed cell proliferation. A decrease of INHBB and Met was detected in regenerating liver.
  
  
  CONCLUSIONS
  MiR-34a expression was upregulated during the late phase of liver regeneration. MiR-34a-mediated regulation of INHBB and Met may collectively contribute to the suppression of hepatocyte proliferation.
authors.affiliation: Department of Biochemistry and Molecular Biology, Second Military Medical University, Shanghai, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Chen
authors.forename: Yimin
authors.initials: Y
authors.lastname: Sun
authors.forename: Ruiqi
authors.initials: R
authors.lastname: Dong
authors.forename: Shengsheng
authors.initials: S
authors.lastname: Yang
authors.forename: Chuanyong
authors.initials: C
authors.lastname: Pan
authors.forename: Dao
authors.initials: D
authors.lastname: Xiang
authors.forename: Mingyong
authors.initials: M
authors.lastname: Miao
authors.forename: Binghua
authors.initials: B
authors.lastname: Jiao
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D049109:Cell Proliferation; D005434:Flow Cytometry; D022781:Hepatocytes / Q000166:cytology* / Q000378:metabolism*; D028322:Inhibin-beta Subunits / Q000235:genetics / Q000378:metabolism; D008115:Liver Regeneration / Q000235:genetics* / Q000502:physiology*; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: C498906:Inhbb protein, rat; D035683:MicroRNAs; D028322:Inhibin-beta Subunits