title: Comparative functional analysis of the human macrophage chitotriosidase.
abstract: This work analyses the chitin-binding and catalytic domains of the human macrophage chitotriosidase and investigates the physiological role of this glycoside hydrolase in a complex mechanism such as the innate immune system, especially its antifungal activity. Accordingly, we first analyzed the ability of its chitin-binding domain to interact with chitin embedded in fungal cell walls using the β-lactamase activity reporter system described in our previous work. The data showed that the chitin-binding activity was related to the cell wall composition of the fungi strains and that their peptide-N-glycosidase/zymolyase treatments increased binding to fungal by increasing protein permeability. We also investigated the antifungal activity of the enzyme against Candida albicans. The antifungal properties of the complete chitotriosidase were analyzed and compared with those of the isolated chitin-binding and catalytic domains. The isolated catalytic domain but not the chitin-binding domain was sufficient to provide antifungal activity. Furthermore, to explain the lack of obvious pathologic phenotypes in humans homozygous for a widespread mutation that renders chitotriosidase inactive, we postulated that the absence of an active chitotriosidase might be compensated by the expression of another human hydrolytic enzyme such as lysozyme. The comparison of the antifungal properties of chitotriosidase and lysozyme indicated that surprisingly, both enzymes have similar in vitro antifungal properties. Furthermore, despite its more efficient hydrolytic activity on chitin, the observed antifungal activity of chitotriosidase was lower than that of lysozyme. Finally, this antifungal duality between chitotriosidase and lysozyme is discussed in the context of innate immunity.
authors.affiliation: Macromolécules Biologiques, Centre d'Ingénierie des Protéines, Institut de Chimie B6a, Université de Liège, Sart-Tilman, Liège, Belgium. marylene.vandevenne@sydney.edu.au
authors.forename: Marylène
authors.initials: M
authors.lastname: Vandevenne
authors.forename: Vincent
authors.initials: V
authors.lastname: Campisi
authors.forename: Astrid
authors.initials: A
authors.lastname: Freichels
authors.forename: Carole
authors.initials: C
authors.lastname: Gillard
authors.forename: Gilles
authors.initials: G
authors.lastname: Gaspard
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Frère
authors.forename: Moreno
authors.initials: M
authors.lastname: Galleni
authors.forename: Patrice
authors.initials: P
authors.lastname: Filée
mesh_terms: D000935:Antifungal Agents / Q000378:metabolism / Q000494:pharmacology; D001665:Binding Sites; D002175:Candida / Q000187:drug effects / Q000378:metabolism; D002473:Cell Wall / Q000378:metabolism; D002686:Chitin / Q000378:metabolism; D006596:Hexosaminidases / Q000378:metabolism* / Q000494:pharmacology*; D006801:Humans; D025301:Hyphae / Q000187:drug effects; D007700:Kinetics; D008264:Macrophages / Q000201:enzymology*; D050296:Microbial Viability / Q000187:drug effects; D009113:Muramidase / Q000378:metabolism / Q000494:pharmacology; D010539:Permeability
chemical_list: D000935:Antifungal Agents; D002686:Chitin; D006596:Hexosaminidases; C086400:chitotriosidase; D009113:Muramidase