title: Highly hydrophilic surfaces from polyglycidol grafts with dual antifouling and specific protein recognition properties.
abstract: Homopolymer grafts from α-tert-butoxy-ω-vinylbenzyl-polyglycidol (PGL) were prepared on gold and stainless steel (SS) substrates modified by 4-benzoyl-phenyl (BP) moieties derived from the electroreduction of the parent salt 4-benzoyl benzene diazonium tetrafluoroborate. The grafted BP aryl groups efficiently served to surface-initiate photopolymerization (SIPP) of PGL. In similar conditions, SIPP of hydroxyethyl methacrylate (HEMA) permitted the production of PHEMA grafts as model surfaces. Water contact angles were found to be 66°, 15°, and 0° for SS-BP, SS-PHEMA, and SS-PPGL, respectively. The spontaneous spreading of water drops on SS-PPGL was invariably observed with 1.5 μL water drops. PPGL thus appears as a superhydrophilic polymer. Resistance to nonspecific adsorption of proteins of PPGL and PHEMA grafts on gold was evaluated by surface plasmon resonance (SPR) using antibovine serum albumin (anti-BSA). The results conclusively show that PPGL-grafts exhibit enhanced resistance to anti-BSA adsorption compared to the well-known hydrophilic PHEMA. PPGL grafts were further modified with BSA through the carbonyldiimidazole activation of the OH groups providing immunosensing surfaces. The so-prepared PPGL-grafted BSA hybrids specifically interacted with anti-BSA in PBS as compared to antimyoglobin. It is clear that the superhydrophilic character of PPGL grafts opens new avenues for biomedical applications where surfaces with dual functionality, namely, specific protein grafting together with resistance to biofouling, are required.
authors.affiliation: ITODYS, University Denis Diderot & CNRS (UMR 7086), 15 rue Jean de Baïf, 75013 Paris, France.
authors.forename: Sarra
authors.initials: S
authors.lastname: Gam-Derouich
authors.forename: Monika
authors.initials: M
authors.lastname: Gosecka
authors.forename: Sandrine
authors.initials: S
authors.lastname: Lepinay
authors.forename: Mireille
authors.initials: M
authors.lastname: Turmine
authors.forename: Benjamin
authors.initials: B
authors.lastname: Carbonnier
authors.forename: Teresa
authors.initials: T
authors.lastname: Basinska
authors.forename: Stanislaw
authors.initials: S
authors.lastname: Slomkowski
authors.forename: Marie-Claude
authors.initials: MC
authors.lastname: Millot
authors.forename: Ali
authors.initials: A
authors.lastname: Othmane
authors.forename: Dalila
authors.initials: D
authors.lastname: Ben Hassen-Chehimi
authors.forename: Mohamed M
authors.initials: MM
authors.lastname: Chehimi
mesh_terms: D000327:Adsorption; D000818:Animals; D057219:Biofouling / Q000517:prevention & control*; D002417:Cattle; D006046:Gold / Q000737:chemistry; D015394:Molecular Structure; D010316:Particle Size; D011409:Propylene Glycols / Q000138:chemical synthesis / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013193:Stainless Steel / Q000737:chemistry; D013499:Surface Properties
chemical_list: D011409:Propylene Glycols; C502907:polyglycidol; D013193:Stainless Steel; D012710:Serum Albumin, Bovine; D006046:Gold