title: Anticancer activity of total flavonoids isolated from Xianhe Yanling Recipe (仙鹤延龄方).
abstract: OBJECTIVE
  To investigate the anticancer activity of the total flavonoids isolated from a herbal formula, Xianhe Yanling Recipe (仙鹤延龄方), a recipe commonly used in cancer patients in China.
  
  
  METHODS
  The in vitro anticancer activity of the total flavonoids was determined using the 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay on three cancer cell lines: MCF-7 (a human breast adenocarcinoma cell line), HepG-2 (a human hepatocellular carcinoma cell line) and ES-2 (a human ovarian cancer cell line). The in vivo anticancer effect of the total flavonoids was assessed in a mouse tumor model bearing H22-induced hepatocellular carcinoma, and cisplatin was used as a positive control.
  
  
  RESULTS
  The total flavonoids exerted a powerful inhibitory effect on the three cell lines, with 50% inhibiting concentrations (IC(50)) of 24.948, 31.569 and 6.923 μg/mL, respectively. In vivo studies showed that the total flavonoids had dose-dependent inhibitory effects on hepatocellular carcinoma in mice.
  
  
  CONCLUSIONS
  The total flavonoids from Xianhe Yanling Recipe have potential anticancer activity, and further researches and development are warranted.
authors.affiliation: Institute of Radiation Medicine, Chinese Academy of Medical Sciences, Peking Union Medical College, Tianjin Key Laboratory of Molecular Nuclear Medicine, Tianjin, China. pharm8888@yahoo.com.cn
authors.forename: Pei-xun
authors.initials: PX
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Gao
authors.forename: Yu-jie
authors.initials: YJ
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Long
authors.forename: Xiu
authors.initials: X
authors.lastname: Shen
authors.forename: Wei-sheng
authors.initials: WS
authors.lastname: Tang
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005419:Flavonoids / Q000302:isolation & purification* / Q000494:pharmacology*; D006801:Humans; D020128:Inhibitory Concentration 50; D051379:Mice; D009368:Neoplasm Transplantation
chemical_list: D000970:Antineoplastic Agents; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; C561425:Xianhe Yanling