title: [Molecular identification of one Uncaria plant].
abstract: OBJECTIVE
  In order to identify a species of Uncaria, molecular phylogenetic analysis was carried out by using the rDNA ITS sequence as molecular marker.
  
  
  METHODS
  Total DNA was extracted from the plant with modified CTAB method and thereby rDNA ITS regions were amplified with universally conserved primer. The rDNA ITS amplicon was characterized by cloning, sequencing, blasting in GenBank and phylogenetic analyses using PAUP by maximum parsimony (MP) criteria.
  
  
  RESULTS
  The rDNA ITS entire sequence of this species of Uncaria was 719 bp. The sequence is related to the U. sinensis available in GenBank and the similarity reaches 99.7%.
  
  
  CONCLUSIONS
  Based on molecular biology methods of rDNA ITS region analysis, molecular identification is available in accurate classification on this species of Uncaria.
authors.affiliation: School of Life Science and Biopharmacology, Guangdong Pharmaceutical University, Guangzhou 510006, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Zhu
authors.forename: Lin
authors.initials: L
authors.lastname: Zhou
authors.forename: Huimin
authors.initials: H
authors.lastname: Pang
authors.forename: Hongliang
authors.initials: H
authors.lastname: Huang
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Gao
authors.forename: Changqing
authors.initials: C
authors.lastname: Zeng
mesh_terms: D018744:DNA, Plant / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D010802:Phylogeny; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D032070:Uncaria / Q000145:classification / Q000235:genetics*
chemical_list: D018744:DNA, Plant; D021903:DNA, Ribosomal Spacer