title: Bioavailability of iron from wheat aegilops derivatives selected for high grain iron and protein contents.
abstract: A coupled in vitro digestion/Caco-2 model was employed to assess iron bioavailability from wheat Aegilops derivatives selected for high iron and protein contents. The iron content in wheat genotypes used in this study correlated to a great extent with both protein (r = 0.80) and phytate (r = 0.68) contents. The iron bioavailability was based on Caco-2 cell ferritin formation from cooked digests of these derivatives (relative to WL711 control) and correlated positively with dialyzable iron (r = 0.63) and total iron content (r = 0.38) but not with the phytate content. The apparently decreased phytate/iron molar ratios, however, correlated negatively (r = -0.42) with the iron bioavailability, justifying the utilization of these parameters in biofortification programs. Iron bioavailability in the derivatives increased up to 1.5-fold, corresponding to a 1.5-2.2-fold increase observed in iron content over control. These data suggest that biofortification for iron proportionately leading to higher iron bioavailability will be the most feasible and cost-effective approach to combat micronutrient deficiency.
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Roorkee, Uttarakhand, India.
authors.forename: Rajani
authors.initials: R
authors.lastname: Salunke
authors.forename: Kumari
authors.initials: K
authors.lastname: Neelam
authors.forename: Nidhi
authors.initials: N
authors.lastname: Rawat
authors.forename: Vijay Kumar
authors.initials: VK
authors.lastname: Tiwari
authors.forename: Gursharn Singh
authors.initials: GS
authors.lastname: Randhawa
authors.forename: Harcharan Singh
authors.initials: HS
authors.lastname: Dhaliwal
authors.forename: Partha
authors.initials: P
authors.lastname: Roy
mesh_terms: D001682:Biological Availability; D018938:Caco-2 Cells; D004063:Digestion; D005293:Ferritins / Q000096:biosynthesis; D005527:Food, Fortified / Q000032:analysis; D005838:Genotype; D006358:Hot Temperature; D006801:Humans; D007501:Iron / Q000032:analysis / Q000493:pharmacokinetics*; D010833:Phytic Acid / Q000032:analysis; D010940:Plant Proteins / Q000032:analysis*; D012639:Seeds / Q000737:chemistry*; D014908:Triticum / Q000737:chemistry* / Q000235:genetics
chemical_list: D010940:Plant Proteins; D010833:Phytic Acid; D005293:Ferritins; D007501:Iron