title: [Preparation of crocin from gardenia yellow pigment by slow rotary countercurrent chromatography].
abstract: A method was established for the preparation of crocin from gardenia yellow pigment by slow rotary countercurrent chromatography (SRCCC). A two-phase solvent system consisting of methyl tert-butyl ether, n-butanol, acetonitrile and water (2 : 2. 5 : 1 : 5, v/v/v/v) was used. The upper phase was used as the stationary phase, and the lower phase as the mobile phase. By SRCCC elution, 2.47 g of crocin with a purity of 96.8% was obtained from 5 g of gardenia yellow pigment at a flow rate of 5 mL/min and a rotation speed of 50 r/min. The results indicated that SRCCC is a powerful technique for the purification of crocin from gardenia yellow pigment with high preparative capacity, high safety and high efficiency in resolution, and it is possible to prepare crocin at industrial level by SRCCC.
authors.affiliation: College of Food Science, Hainon University, Haikou 570228, China. jzg6666@sina.com
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Jiang
authors.forename: Wenxue
authors.initials: W
authors.lastname: Chen
authors.forename: Sixin
authors.initials: S
authors.lastname: Liu
authors.forename: Qizhen
authors.initials: Q
authors.lastname: Du
mesh_terms: D020001:1-Butanol / Q000737:chemistry; D002338:Carotenoids / Q000737:chemistry / Q000302:isolation & purification*; D003377:Countercurrent Distribution / Q000379:methods*; D032062:Gardenia / Q000737:chemistry*; D008738:Methyl Ethers / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D008738:Methyl Ethers; D010936:Plant Extracts; C043243:methyl tert-butyl ether; D002338:Carotenoids; C029036:crocin; D020001:1-Butanol; C057653:gardenia yellow