title: Molecular cloning and mRNA expression of the liver-specific cathepsin L1 gene of the olive flounder, Paralichthys olivaceus.
abstract: We isolated a homolog of cathepsin L from a cDNA library of the olive flounder liver. The flounder cathepsin L1 transcript consisted of 1,221 bp that encoded a polypeptide of 334 amino acids. The overall identity between flounder cathepsin L1 and other cathepsin Ls was 50-64%, and flounder cathepsin L1 contained the highly conserved ERFNIN-motif. A phylogenetic tree indicated that flounder cathepsin L1 is in the same monophyletic group as zebrafish cathepsin Lc. RT-PCR analysis revealed that cathepsin L1 transcripts were expressed only in the liver. They were detected from 28 d post-hatching. Under starvation conditions, cathepsin L1 expression was decreased at 30 d.
authors.affiliation: Biotechnology Research Division, National Fisheries Research and Development Institute, Gijang, Busan, Republic of Korea. yokim@nfrdi.go.kr
authors.forename: Young-Ok
authors.initials: YO
authors.lastname: Kim
authors.forename: Eun-Mi
authors.initials: EM
authors.lastname: Park
authors.forename: Jung Soo
authors.initials: JS
authors.lastname: Seo
authors.forename: Bo-Hye
authors.initials: BH
authors.lastname: Nam
authors.forename: Hee Jeong
authors.initials: HJ
authors.lastname: Kong
authors.forename: Woo-Jin
authors.initials: WJ
authors.lastname: Kim
authors.forename: Bong-Seok
authors.initials: BS
authors.lastname: Kim
authors.forename: Kyung-Kil
authors.initials: KK
authors.lastname: Kim
authors.forename: Sang-Jun
authors.initials: SJ
authors.lastname: Lee
mesh_terms: D020816:Amino Acid Motifs; D000818:Animals; D056668:Cathepsin L / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D004926:Escherichia coli; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism*; D005432:Flounder / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D015723:Gene Library; D006801:Humans; D008099:Liver / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010957:Plasmids; D012333:RNA, Messenger; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D014169:Transformation, Bacterial
chemical_list: D029941:Fish Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D056668:Cathepsin L