title: The rate of O₂ loss from mesenteric arterioles is not unusually high.
abstract: The O(2) disappearance curve (ODC) recorded in an arteriole after the rapid arrest of blood flow reflects the complex interaction among the dissociation of O(2) from hemoglobin, O(2) diffusivity, and rate of respiration in the vascular wall and surrounding tissue. In this study, the analysis of experimental ODCs allowed the estimation of parameters of O(2) transport and O(2) consumption in the microcirculation of the mesentery. We collected ODCs from rapidly arrested blood inside rat mesenteric arterioles using scanning phosphorescence quenching microscopy (PQM). The technique was used to prevent the artifact of accumulated O(2) photoconsumption in stationary media. The observed ODC signatures were close to linear, in contrast to the reported exponential decline of intra-arteriolar Po(2). The rate of Po(2) decrease was 0.43 mmHg/s in 20-μm-diameter arterioles. The duration of the ODC was 290 s, much longer than the 12.8 s reported by other investigators. The arterioles associated with lymphatic microvessels had a higher O(2) disappearance rate of 0.73 mmHg/s. The O(2) flux from arterioles, calculated from the average O(2) disappearance rate, was 0.21 nl O(2)·cm(-2)·s(-1), two orders of magnitude lower than reported in the literature. The physical upper limit of the O(2) consumption rate by the arteriolar wall, calculated from the condition that all O(2) is consumed by the wall, was 452 nl O(2)·cm(-3)·s(-1). From consideration of the microvascular tissue volume fraction in the rat mesentery of 6%, the estimated respiration rate of the vessel wall was ∼30 nl O(2)·cm(-3)·s(-1). This result was three orders of magnitude lower than the respiration rate in rat mesenteric arterioles reported by other investigators. Our results demonstrate that O(2) loss from mesenteric arterioles is small and that the O(2) consumption by the arteriolar wall is not unusually large.
authors.affiliation: Department of Physiology, Medical College of Virginia Campus, Virginia Commonwealth University, Richmond, Virginia 23298-0551, USA.
authors.forename: Aleksander S
authors.initials: AS
authors.lastname: Golub
authors.forename: Bjorn K
authors.initials: BK
authors.lastname: Song
authors.forename: Roland N
authors.initials: RN
authors.lastname: Pittman
mesh_terms: D000818:Animals; D001160:Arterioles / Q000378:metabolism; D001783:Blood Flow Velocity; D019069:Cell Respiration; D007700:Kinetics; D016014:Linear Models; D008297:Male; D008643:Mesentery / Q000098:blood supply*; D008833:Microcirculation; D036641:Microscopy, Fluorescence, Multiphoton; D008955:Models, Cardiovascular; D010100:Oxygen / Q000097:blood*; D010101:Oxygen Consumption; D051381:Rats; D017207:Rats, Sprague-Dawley; D013152:Splanchnic Circulation
chemical_list: D010100:Oxygen