title: Precision across the analytical measuring range of a quantitative real-time PCR assay for cytomegalovirus detection among three clinical laboratories.
abstract: This study measured the precision of a quantitative laboratory-developed real-time PCR test for cytomegalovirus performed at three different clinical laboratories that use the same methodology. The overall standard deviation (adjusted for analyte level) was 0.18 log(10) copies/ml, and there was no significant relationship between standard deviation and analytical measuring range.
authors.affiliation: Molecular Microbiology Laboratory, Mayo Medical Laboratories New England, 160 Dascomb Rd., Andover, MA 01810, USA. grys.thomas@mayo.edu
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Grys
authors.forename: Doreen L
authors.initials: DL
authors.lastname: Duquette
authors.forename: Bruce
authors.initials: B
authors.lastname: White
authors.forename: Cole
authors.initials: C
authors.lastname: Irish
authors.forename: D Jane
authors.initials: DJ
authors.lastname: Hata
authors.forename: Bobbi S
authors.initials: BS
authors.lastname: Pritt
mesh_terms: D019411:Clinical Laboratory Techniques / Q000592:standards*; D003587:Cytomegalovirus / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000175:diagnosis* / Q000453:epidemiology*; D006801:Humans; D015588:Observer Variation; D060888:Real-Time Polymerase Chain Reaction / Q000592:standards*; D014773:Virology / Q000592:standards*