title: Effects of electromagnetic stimulation on osteogenic differentiation of human mesenchymal stromal cells seeded onto gelatin cryogel.
abstract: Bone tissue engineering typically uses biomaterial scaffolds, osteoblasts or cells that can become osteoblasts, and biophysical stimulations to promote cell attachment and differentiation. In this study, we investigated the effects of an electromagnetic wave on mesenchymal stromal cells isolated from the bone marrow and seeded upon gelatin cryogel disks. In comparison with control conditions without electromagnetic stimulus, the electromagnetic treatment (magnetic field, 2 mT; frequency, 75 Hz) increased the cell proliferation and differentiation and enhanced the biomaterial surface coating with bone extracellular matrix proteins. Using this tissue-engineering approach, the gelatin biomaterial, coated with differentiated cells and their extracellular matrix proteins, may be used in clinical applications as an implant for bone defect repair.
authors.affiliation: Dipartimento di Biochimica - Sez, Medicina e Farmacia, University of Pavia, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Saino
authors.forename: L
authors.initials: L
authors.lastname: Fassina
authors.forename: S
authors.initials: S
authors.lastname: Van Vlierberghe
authors.forename: M A
authors.initials: MA
authors.lastname: Avanzini
authors.forename: P
authors.initials: P
authors.lastname: Dubruel
authors.forename: G
authors.initials: G
authors.lastname: Magenes
authors.forename: L
authors.initials: L
authors.lastname: Visai
authors.forename: F
authors.initials: F
authors.lastname: Benazzo
mesh_terms: D000818:Animals; D001857:Bone Matrix / Q000378:metabolism / Q000528:radiation effects; D002417:Cattle; D002454:Cell Differentiation / Q000528:radiation effects*; D059985:Cryogels; D003470:Culture Media; D004247:DNA / Q000032:analysis / Q000096:biosynthesis; D004574:Electromagnetic Fields; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D005780:Gelatin; D006801:Humans; D020100:Hydrogels; D059630:Mesenchymal Stem Cells / Q000528:radiation effects*; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000528:radiation effects; D010012:Osteogenesis / Q000528:radiation effects*; D017154:Stromal Cells / Q000528:radiation effects*; D023822:Tissue Engineering / Q000379:methods
chemical_list: D059985:Cryogels; D003470:Culture Media; D016326:Extracellular Matrix Proteins; D020100:Hydrogels; D005780:Gelatin; D004247:DNA