title: Enzymatic properties of recombinant kojibiose phosphorylase from Caldicellulosiruptor saccharolyticus ATCC43494.
abstract: One kojibiose phoshorylase (KP) homolog gene was cloned from Caldicellulosiruptor saccharolyticus ATCC43494. Recombinant KP from C. saccharolyticus (Cs-KP) expressed in Escherichia coli showed highest activity at pH 6.0 at 85 °C, and was stable from pH 3.5 to 10.0 and up to 85 °C for phosphorolysis. Cs-KP showed higher productivity of kojioligosaccharides of DP ≧ 4 than KP from Thermoanaerobacter brockii ATCC35047.
authors.affiliation: Glycoscience Institute, Research Center, Hayashibara Biochemical Laboratories, Inc., Naka-ku, Okayama, Japan. takyamamoto@hayashibara.co.jp
authors.forename: Takuo
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Mio
authors.initials: M
authors.lastname: Nishio-Kosaka
authors.forename: Seisuke
authors.initials: S
authors.lastname: Izawa
authors.forename: Hajime
authors.initials: H
authors.lastname: Aga
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Nishimoto
authors.forename: Hiroto
authors.initials: H
authors.lastname: Chaen
authors.forename: Shigeharu
authors.initials: S
authors.lastname: Fukuda
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D004187:Disaccharides / Q000378:metabolism*; D004926:Escherichia coli; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D006005:Phosphorylases / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*; D013379:Substrate Specificity; D046950:Thermoanaerobacterium / Q000737:chemistry / Q000201:enzymology*; D014169:Transformation, Bacterial
chemical_list: D001426:Bacterial Proteins; D004187:Disaccharides; D011994:Recombinant Proteins; C008237:kojibiose; D006005:Phosphorylases