title: GABAergic projections from the subplate to Cajal-Retzius cells in the neocortex.
abstract: Subplate neurons and Cajal-Retzius cells play an important role in the corticogenesis. Despite morphological evidence, the question whether subplate neurons innervate Cajal-Retzius cells has not been studied yet. We report that electrical stimulation in the subplate resulted in evoked GABAergic inhibitory postsynaptic currents (eIPSCs) in Cajal-Retzius cells. The eIPSC latency showed minor variability and amounted to approximately 4 ms, suggesting the monosynaptic connection. During the first postnatal week: (i) eIPSC amplitude increased, (ii) eIPSC kinetics sped up, (iii) the size of readily releasable pool increased, and (iv) γ-aminobutyric acid release probability decreased. We conclude that GABAergic subplate neurons innervate Cajal-Retzius cells. Surprisingly, despite the transient nature of both cell populations, these projections show developmental adjustments typical for many nontransient synaptic connections.
authors.affiliation: Institute of Physiology and Pathophysiology, Universal Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany.
authors.forename: Olga
authors.initials: O
authors.lastname: Myakhar
authors.forename: Petr
authors.initials: P
authors.lastname: Unichenko
authors.forename: Sergei
authors.initials: S
authors.lastname: Kirischuk
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D003627:Data Interpretation, Statistical; D004558:Electric Stimulation; D055724:Electrophysiological Phenomena; D019706:Excitatory Postsynaptic Potentials / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D019579:Neocortex / Q000166:cytology* / Q000254:growth & development*; D009434:Neural Pathways / Q000166:cytology / Q000254:growth & development / Q000502:physiology; D009474:Neurons / Q000502:physiology*; D018408:Patch-Clamp Techniques; D013569:Synapses / Q000502:physiology; D009435:Synaptic Transmission / Q000502:physiology; D005680:gamma-Aminobutyric Acid / Q000502:physiology*
chemical_list: D005680:gamma-Aminobutyric Acid