title: Determination of quinolone residues in infant and young children powdered milk combining solid-phase extraction and ultra-performance liquid chromatography-tandem mass spectrometry.
abstract: The present work describes a method based on solid-phase extraction (SPE) and ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) for the simultaneous determination of three quinolones (pipemidic acid, oxolinic acid and flumequine) and twelve fluoroquinolones (marbofloxacin, fleroxacin, pefloxacin, levofloxacin, norfloxacin, ciprofloxacin, enrofloxacin, danofloxacin, lomefloxacin, difloxacin, sarafloxacin, and moxifloxacin) in different infant and young children powdered milks. After suitable deproteination of the reconstituted powdered samples, a SPE procedure was developed providing recovery values higher than 84% (RSDs lower than 13%) for all the analytes, with limits of detection between 0.04 and 0.52 μg/kg. UPLC-MS/MS analyses were carried out in less than 10 min. Sixteen infant and young children powdered milk samples of different origin, type and composition bought at Spanish markets were analyzed. Residues of the selected antibiotics were not detected in any of the analyzed samples.
authors.affiliation: Department of Analytical Chemistry, Nutrition and Food Science, Universidad de La Laguna (ULL). Avenida Astrofísico Francisco Sánchez, s/n. 38206 La Laguna (Tenerife), Spain.
authors.forename: Antonio V
authors.initials: AV
authors.lastname: Herrera-Herrera
authors.forename: Javier
authors.initials: J
authors.lastname: Hernández-Borges
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Rodríguez-Delgado
authors.forename: Miguel
authors.initials: M
authors.lastname: Herrero
authors.forename: Alejandro
authors.initials: A
authors.lastname: Cifuentes
mesh_terms: D000097:Acetonitriles; D000900:Anti-Bacterial Agents / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis*; D024841:Fluoroquinolones / Q000032:analysis*; D006801:Humans; D007223:Infant; D041943:Infant Formula / Q000737:chemistry*; D010093:Oxolinic Acid / Q000032:analysis; D010876:Pipemidic Acid / Q000032:analysis; D012044:Regression Analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D000097:Acetonitriles; D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D010093:Oxolinic Acid; D010876:Pipemidic Acid; C032159:acetonitrile