title: Infrared spectromicroscopy of biochemistry in functional single cells.
abstract: Over the years Fourier-Transform Infrared (FTIR) spectroscopy has been widely employed in the structural and functional characterization of biomolecules. The introduction of infrared (IR) microscopes and of synchrotron light sources has created expectations that FTIR could become a generally viable technique to study both structure and reactivity in vivo, inside single cells, by performing measurements that up to a few years ago were the preserve of in vitro experiments on purified macromolecules. In this review we present the state-of-the-art in the application of FTIR spectromicroscopy as a technique for the study of structure and dynamics in single cells, we discuss the performance requirements for this application and review developments in sample handling methods.
authors.affiliation: Swiss Light Source, Paul Scherrer Institut, Villigen-PSI, CH-5232, Switzerland. luca.quaroni@psi.ch
authors.forename: Luca
authors.initials: L
authors.lastname: Quaroni
authors.forename: Theodora
authors.initials: T
authors.lastname: Zlateva
mesh_terms: D016825:Chlamydomonas reinhardtii / Q000145:classification / Q000166:cytology; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D012243:Rhodopsin / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D014867:Water / Q000737:chemistry
chemical_list: D014867:Water; D012243:Rhodopsin