title: Controlled confinement of DNA at the nanoscale: nanofabrication and surface bio-functionalization.
abstract: Nanopatterned arrays of biomolecules are a powerful tool to address fundamental issues in many areas of biology. DNA nanoarrays, in particular, are of interest in the study of DNA-protein interactions and for biodiagnostic investigations. In this context, achieving a highly specific nanoscale assembly of oligonucleotides at surfaces is critical. In this chapter, we describe a method to control the immobilization of DNA on nanopatterned surfaces; the nanofabrication and the bio-functionalization involved in the process will be discussed.
authors.affiliation: Department of Mechanical Engineering & Applied Physics and Applied Mathematics, Columbia University, New York, NY, USA. mp2766@columbia.edu
authors.forename: Matteo
authors.initials: M
authors.lastname: Palma
authors.forename: Justin J
authors.initials: JJ
authors.lastname: Abramson
authors.forename: Alon A
authors.initials: AA
authors.lastname: Gorodetsky
authors.forename: Colin
authors.initials: C
authors.lastname: Nuckolls
authors.forename: Michael P
authors.initials: MP
authors.lastname: Sheetz
authors.forename: Shalom J
authors.initials: SJ
authors.lastname: Wind
authors.forename: James
authors.initials: J
authors.lastname: Hone
mesh_terms: D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism; D005898:Glass / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D007103:Immobilization / Q000379:methods; D018625:Microscopy, Atomic Force; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D010165:Palladium / Q000737:chemistry; D013499:Surface Properties
chemical_list: D004268:DNA-Binding Proteins; D010165:Palladium; D006046:Gold; D004247:DNA