title: The missing C-17 O-methyltransferase in geldanamycin biosynthesis.
abstract: The biosynthetic gene clusters for the Hsp90 inhibitor geldanamycin (GDM, 1) have been cloned previously from three different Streptomyces strains, but the gene encoding the C-17 O-methyltransferase remains unknown. The cloning and sequencing of a new GDM biosynthetic gene cluster from Streptomyces autolyticus CGMCC 0516 was reported, identifying the gdmMT gene that encodes the missing C-17 O-methyltransferase for 1 biosynthesis.
authors.affiliation: Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, China.
authors.forename: Min
authors.initials: M
authors.lastname: Yin
authors.forename: Tao
authors.initials: T
authors.lastname: Lu
authors.forename: Li-Xing
authors.initials: LX
authors.lastname: Zhao
authors.forename: Yihua
authors.initials: Y
authors.lastname: Chen
authors.forename: Sheng-Xiong
authors.initials: SX
authors.lastname: Huang
authors.forename: Jeremy R
authors.initials: JR
authors.lastname: Lohman
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Xu
authors.forename: Cheng-Lin
authors.initials: CL
authors.lastname: Jiang
authors.forename: Ben
authors.initials: B
authors.lastname: Shen
mesh_terms: D016227:Benzoquinones / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D002384:Catalysis; D018841:HSP90 Heat-Shock Proteins / Q000037:antagonists & inhibitors*; D047029:Lactams, Macrocyclic / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D008780:Methyltransferases / Q000235:genetics* / Q000378:metabolism*; D015394:Molecular Structure; D005810:Multigene Family; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*
chemical_list: D016227:Benzoquinones; D018841:HSP90 Heat-Shock Proteins; D047029:Lactams, Macrocyclic; D008780:Methyltransferases; C001277:geldanamycin