title: Longitudinal analysis and genotyping of infant dominant bifidobacterial populations.
abstract: Bifidobacterial population dynamics were investigated by the longitudinal analysis of the dominant population isolated from the feces of young infants. After molecular identification and fingerprinting comparison, clone identity of the consecutive strains belonging to the same species for one individual was performed by pulsed-field gel electrophoresis. The results, obtained from 15 individuals sampled four times over a five-week period suggested a turnover of the dominant bifidobacteria in the population not only at the species but also at its species representative levels. This study provides new insights of the in vivo dynamics of commensal bifidobacteria. It highlights the need to take into consideration the fluctuation of bifidobacterial populations that may occur in one individual in order to investigate reliably the impact of dietary components, such as probiotics or prebiotics, on the intestinal ecosystem.
authors.affiliation: Université Paris Descartes, EA4065, Faculté des Sciences Pharmaceutiques et Biologiques, Paris, France. julio.aires@parisdescartes.fr
authors.forename: J
authors.initials: J
authors.lastname: Aires
authors.forename: M
authors.initials: M
authors.lastname: Thouverez
authors.forename: S
authors.initials: S
authors.lastname: Allano
authors.forename: M J
authors.initials: MJ
authors.lastname: Butel
mesh_terms: D058491:Bacterial Load; D015373:Bacterial Typing Techniques; D001644:Bifidobacterium / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D046508:Culture Techniques; D016521:Electrophoresis, Gel, Pulsed-Field; D005243:Feces / Q000382:microbiology; D060005:Genotyping Techniques; D006801:Humans; D007223:Infant; D007422:Intestines / Q000382:microbiology*; D008137:Longitudinal Studies; D054892:Metagenome; D019936:Probiotics; D013045:Species Specificity; D013559:Symbiosis; D013997:Time Factors