title: [Epithelial intestine cells transdifferentiate into bladder urothelium in experiments in vivo].
abstract: The autoplastic surgery by intestine tissue has been used for reconstructive therapy of the urinary tract since the middle of the last century; however, cell mechanisms of the urothelium engraftment are still obscure. Intestine stem cells possess plasticity and presumably enable after the autoplastic surgery to transdifferentiate into mature cells of urinary tract. Using the preliminary developed in vivo model for evaluation of somatic cells transdifferentiation into urothelium, we have found that the epithelial intestine cells producing Gfp transdifferentiate into the cryoinjured bladder urothelium of the syngenetic C57BL mice. Gfp was detected in the bladder tissue of mice-recipients using reverted polymerase chain reaction, primary fluorescence and immunofluorescence, while colocalization of the Gfp and Her-4 revealing similar to urothelium staining pattern was demonstrated in a few urothelium cells by double immunohistochemical staining of the bladder tissue with specific antibodies. The results obtained suggest that epithelial intestine cells enable to transdifferentiate into bladder urothelium, however the transdifferentiation level is low and presumably can not provide full functional urothelium engraftment in the case of autoplastic bladder surgery by intestine tissue.
authors.forename: B K
authors.initials: BK
authors.lastname: Popov
authors.forename: A M
authors.initials: AM
authors.lastname: Zaĭchik
authors.forename: M B
authors.initials: MB
authors.lastname: Bud'ko
authors.forename: O V
authors.initials: OV
authors.lastname: Zlobina
authors.forename: E N
authors.initials: EN
authors.lastname: Tolkunova
authors.forename: O V
authors.initials: OV
authors.lastname: Zhidkova
authors.forename: N S
authors.initials: NS
authors.lastname: Petrov
mesh_terms: D000818:Animals; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors / Q000235:genetics / Q000378:metabolism; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism; D015153:Blotting, Western; D054338:Cell Transdifferentiation; D004815:Epidermal Growth Factor / Q000235:genetics / Q000378:metabolism; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism; D066246:ErbB Receptors / Q000235:genetics / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D049452:Green Fluorescent Proteins / Q000032:analysis; D007150:Immunohistochemistry; D015552:Injections, Intralesional; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000166:cytology; D051379:Mice; D008822:Mice, Transgenic; D066247:Receptor, ErbB-4; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D000073940:Transcription Factor 4; D014184:Transplantation, Homologous; D001743:Urinary Bladder / Q000166:cytology / Q000293:injuries; D019459:Urothelium / Q000166:cytology* / Q000378:metabolism; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism
chemical_list: D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; D051792:Basic Helix-Loop-Helix Proteins; D004815:Epidermal Growth Factor; D066246:ErbB Receptors; D049452:Green Fluorescent Proteins; D066247:Receptor, ErbB-4; D000073940:Transcription Factor 4; D051176:beta Catenin; C075668:Tcf3 protein, mouse; C090743:Tcf4 protein, mouse; C587690:Erbb4 protein, mouse