title: The relationship between exhaled carbon monoxide and human neutrophil function in the Japanese general population.
abstract: We have evaluated the relationship between exhaled carbon monoxide (CO) level and neutrophil-related functions such as reactive oxygen species (ROS) production capability, phagocytic activity and serum opsonic activity in the general population. Serum opsonic activity was determined by measuring the effects of serum on neutrophil ROS production capability using lucigenin- and luminol-dependent chemiluminescence (LgCL, LmCL). LgCL is associated with the detection of O(2)(-) , whereas LmCL mainly detects H(2)O(2) and HOCl, which are higher reactive oxygen radicals. In females, exhaled CO level was found to have positive associations with ROS production capability and LgCL. However, the opposite tendency was seen between exhaled CO level and LmCL in both genders. This result suggests that neutrophil ROS production in females may have contributed to oxidative stress, which led to the increases in intrinsic CO and exhaled CO consequently. Such changes then may have inhibited the process of changing reactive oxygen radicals into higher oxidizing potential levels.
authors.affiliation: Department of Social Medicine, Hirosaki University Graduate School of Medicine, Hirosaki, 036-8562 Aomori, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Mikami
authors.forename: Ippei
authors.initials: I
authors.lastname: Takahashi
authors.forename: Masashi
authors.initials: M
authors.lastname: Matsuzaka
authors.forename: Kazuma
authors.initials: K
authors.lastname: Danjo
authors.forename: Kiyonori
authors.initials: K
authors.lastname: Yamai
authors.forename: Ryo
authors.initials: R
authors.lastname: Inoue
authors.forename: Kaori
authors.initials: K
authors.lastname: Iwane
authors.forename: Takashi
authors.initials: T
authors.lastname: Umeda
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Nakaji
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001944:Breath Tests; D002248:Carbon Monoxide / Q000032:analysis* / Q000378:metabolism; D045853:Exhalation; D005260:Female; D006801:Humans; D007564:Japan; D008163:Luminescent Measurements; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D055815:Young Adult
chemical_list: D017382:Reactive Oxygen Species; D002248:Carbon Monoxide