title: Effect of carbon source on polysaccharide production by alginate-entrapped Aureobasidium pullulans ATCC 42023 cells.
abstract: The production of the exopolysaccharide pullulan using entrapped cells of the fungus Aureobasidium pullulans ATCC 42023 was investigated relative to carbon source. Fungal cells grown on glucose or sucrose as a carbon source were entrapped in calcium alginate beads and found to be capable of synthesizing the polysaccharide for two production cycles. Using 2.5% glucose or sucrose as a carbon source, productivity was 18.3 or 21.9 mg polysaccharide/g cells × h, respectively after the initial production cycle and decreased to 9.6 or 8.5 mg polysaccharide/g cells × h, respectively, after the second production cycle. Independent of carbon source, the entrapped fungal cells exhibited a higher yield during the initial cycle than the second production cycle while the entrapped ATCC 42023 cells elaborated a polysaccharide with a higher pullulan content during the second production cycle compared to the initial production cycle.
authors.affiliation: Department of Biology and Microbiology, South Dakota State University, Brookings, SD 57007, USA. Thomas.West@sdstate.edu
authors.forename: Thomas P
authors.initials: TP
authors.lastname: West
mesh_terms: D000464:Alginates; D001203:Ascomycota / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism; D018914:Cells, Immobilized / Q000378:metabolism*; D005936:Glucans / Q000096:biosynthesis* / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D013395:Sucrose / Q000378:metabolism*
chemical_list: D000464:Alginates; D005936:Glucans; D006603:Hexuronic Acids; D013395:Sucrose; D002244:Carbon; D020723:Glucuronic Acid; C009109:pullulan; D005947:Glucose