title: Removal of enzymatic and fermentation inhibitory compounds from biomass slurries for enhanced biorefinery process efficiencies.
abstract: Within the biorefinery paradigm, many non-monomeric sugar compounds have been shown to be inhibitory to enzymes and microbial organisms that are used for hydrolysis and fermentation. Here, two novel separation technologies, polyelectrolyte polymer adsorption and resin-wafer electrodeionization (RW-EDI), have been evaluated to detoxify a dilute acid pretreated biomass slurry. Results showed that detoxification of a dilute acid pretreated ponderosa pine slurry by sequential polyelectrolyte and RW-EDI treatments was very promising, with significant removal of acetic acid, 5-hydroxymethyl furfural, and furfural (up to 77%, 60%, and 74% removed, respectively) along with >97% removal of sulfuric acid. Removal of these compounds increased the cellulose conversion to 94% and elevated the hydrolysis rate to 0.69 g glucose/L/h. When using Saccharomyces cerevisiae D(5)A for fermentation of detoxified slurry, the process achieved 99% of the maximum theoretical ethanol yield and an ethanol production rate nearly five-times faster than untreated slurry.
authors.affiliation: Department of Chemical and Biological Engineering, South Dakota School of Mines and Technology, Rapid City, SD 57701, USA.
authors.forename: Raghu N
authors.initials: RN
authors.lastname: Gurram
authors.forename: Saurav
authors.initials: S
authors.lastname: Datta
authors.forename: Yupo J
authors.initials: YJ
authors.lastname: Lin
authors.forename: Seth W
authors.initials: SW
authors.lastname: Snyder
authors.forename: Todd J
authors.initials: TJ
authors.lastname: Menkhaus
mesh_terms: D000327:Adsorption; D018533:Biomass; D002851:Chromatography, High Pressure Liquid; D004791:Enzyme Inhibitors / Q000302:isolation & purification*; D005285:Fermentation; D006868:Hydrolysis; D011094:Polyethyleneimine / Q000737:chemistry
chemical_list: D004791:Enzyme Inhibitors; D011094:Polyethyleneimine