title: Identification and cross-species transferability of 112 novel unigene-derived microsatellite markers in tea (Camellia sinensis).
abstract: OBJECTIVE
  Tea Unigene-derived MicroSatellite (TUGMS) markers were identified from the publicly available EST data in Camellia sinensis for characterization and future genome mapping studies in tea.
  
  
  RESULTS
  One hundred twelve novel TUGMS markers were identified from 4356 unigenes derived by clustering of 12788 random ESTs in C. sinensis. Amplification-based validation of the TUGMS loci proved them to be highly polymorphic [an average (av.) of 5.24 alleles], heterozygous (H(E), av. 0.746; H(o), av. 0.566) and informative (PIC, av. 0.392). TUGMS loci were 100% transferable in cultivated C. assamica and C. assamica subsp. lasiocalyx and highly cross-transferrable to the related species C. japonica, C. rosiflora, and C. sasanqua.
  
  
  CONCLUSIONS
  These 112 novel highly polymorphic TUGMS markers with proven cross-species transferability will facilitate the future genetic diversity and genome mapping studies in tea.
authors.affiliation: Biotechnology Division, Institute of Himalayan Bioresource Technology, IHBT (Council of Scientific & Industrial Research), Post Box 6, Palampur, Himachal Pradesh 176061, India.
authors.forename: Himanshu
authors.initials: H
authors.lastname: Sharma
authors.forename: Rahul
authors.initials: R
authors.lastname: Kumar
authors.forename: Vikas
authors.initials: V
authors.lastname: Sharma
authors.forename: Vishal
authors.initials: V
authors.lastname: Kumar
authors.forename: Pankaj
authors.initials: P
authors.lastname: Bhardwaj
authors.forename: Paramvir Singh
authors.initials: PS
authors.lastname: Ahuja
authors.forename: Ram Kumar
authors.initials: RK
authors.lastname: Sharma
mesh_terms: D028241:Camellia sinensis / Q000235:genetics*; D017343:Genes, Plant / Q000235:genetics*; D005819:Genetic Markers; D018895:Microsatellite Repeats / Q000235:genetics*; D013045:Species Specificity; D013662:Tea / Q000235:genetics*
chemical_list: D005819:Genetic Markers; D013662:Tea