title: Toll-like receptor 9 polymorphism in patients with erythema multiforme, Stevens Johnson syndrome and Stevens Johnson syndrome/toxic epidermal necrolysis overlap syndrome.
abstract: BACKGROUND
  "Toll like receptor" (TLR) 9 functions in stepping in of native immune system against different viral and bacterial pathogens and induction of adaptive immune response effectively. TLR 9 gene polymorphism makes host predisposed to microbial pathogens by affecting thefunctional capabilities of the receptor.
  
  
  OBJECTIVE
  We aimed to determine if TLR 9 gene polymorphism makes a predisposition to "erythema multiforme" (EM), "Stevens Johnson syndrome" (SJS) and "Stevens Johnson syndrome/toxic epidermal necrolysis overlap syndrome" (SJS/TEN).
  
  
  METHODS
  Forty-two patients clinically and/or histopathologically diagnosed as EM, SJS, and SJS/TEN overlap syndrome and 50 healthy control subjects were enrolled in our study. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was applied for TLR 9 gene 1237 thymine/cytosine (T/C) polymorphism. Genotypes were determined according to bands occurring on agarose gel electrophoresis.
  
  
  RESULTS
  In patients group, the frequencies of TT and TC genotypes were 73.8% and 26.2% while CC genotype wasn't detected. In control group, the frequencies of TT, TC and CC genotypes were 74%, 24%, and 2%. There wasn't a statistically significant difference for TT, TC and CC genotypes between patients and controls. The frequencies of T and C alleles were 84.5% and 15.5% in patients and 86% and 14% in controls, respectively.
  
  
  CONCLUSIONS
  Our results showed that there isn't any association between TLR gene polymorphism and EM, SJS, SJS/TEN overlap syndrome (Tab. 1, Fig. 1, Ref. 30).
authors.affiliation: Uludag University Medical Faculty, Department of Medical Genetics, Bursa, Turkey.
authors.forename: H
authors.initials: H
authors.lastname: Turan
authors.forename: E
authors.initials: E
authors.lastname: Bulbul Baskan
authors.forename: T
authors.initials: T
authors.lastname: Yakut
authors.forename: M
authors.initials: M
authors.lastname: Karkucak
authors.forename: S
authors.initials: S
authors.lastname: Tunali
authors.forename: H
authors.initials: H
authors.lastname: Saricaoglu
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D004892:Erythema Multiforme / Q000235:genetics* / Q000276:immunology; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013262:Stevens-Johnson Syndrome / Q000235:genetics* / Q000276:immunology; D051217:Toll-Like Receptor 9 / Q000235:genetics*; D055815:Young Adult
chemical_list: D051217:Toll-Like Receptor 9