title: Interleukin-1 beta affects cyclooxygenase-2 expression and cartilage metabolism in mandibular condyle.
abstract: Extracellular matrix degradation in mandibular condylar cartilage is mediated by various cytokines in the temporomandibular joint (TMJ). Interleukin-1 beta (IL-1β) is detected in joint structures with pathologic status, and participates in catabolic action in the extracellular matrix. The purpose of this study was to investigate the effects of IL-1β on cyclooxygenase-2 (COX-2) expression and cartilage metabolism using cultured chondrocytes from mandibular condyle. Articular chondrocytes from the porcine mandibular condylar cartilage around the surface were cultured and treated with 0-10 ng/ml IL-1β or 0-1000 ng/ml prostaglandin (PGE(2)) for 0-24h. The mRNA levels of COX-2, MMP-1, -3, and -13 were evaluated by real-time PCR analysis. The protein levels of PGE(2) and MMPs were examined by ELISA and Western blot analysis, respectively. The expression levels of COX-2 and PGE(2) were enhanced by exogenous IL-1β in chondrocytes. The mRNA levels of MMP-1, -3, and -13 were up-regulated by PGE(2) treatment dose-dependently. It is shown that the expression of COX-2/PGE(2) was enhanced by IL-1β in articular chondrocytes from mandibular condyle, and that MMP-1, -3, and -13 were induced by PGE(2), suggesting that IL-1β-induced COX-2/PGE(2) play a crucial role in catabolic processes of mandibular condylar cartilage under inflammatory conditions.
authors.affiliation: Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical Sciences, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, Japan. tkotaro@hiroshima-u.ac.jp
authors.forename: Kotaro
authors.initials: K
authors.lastname: Tanimoto
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Iwabuchi
authors.forename: Yuki
authors.initials: Y
authors.lastname: Tanne
authors.forename: Takashi
authors.initials: T
authors.lastname: Kamiya
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Inubushi
authors.forename: Ryo
authors.initials: R
authors.lastname: Kunimatsu
authors.forename: Tomomi
authors.initials: T
authors.lastname: Mitsuyoshi
authors.forename: Kazuo
authors.initials: K
authors.lastname: Tanne
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000187:drug effects* / Q000378:metabolism; D002478:Cells, Cultured; D019902:Chondrocytes / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000096:biosynthesis*; D015232:Dinoprostone / Q000096:biosynthesis* / Q000494:pharmacology*; D004790:Enzyme Induction; D005260:Female; D053583:Interleukin-1beta / Q000494:pharmacology*; D008335:Mandibular Condyle / Q000187:drug effects / Q000378:metabolism*; D020781:Matrix Metalloproteinase 1 / Q000096:biosynthesis; D053509:Matrix Metalloproteinase 13 / Q000096:biosynthesis; D019278:Matrix Metalloproteinase 3 / Q000096:biosynthesis; D060888:Real-Time Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000494:pharmacology; D034421:Sus scrofa; D013704:Temporomandibular Joint / Q000187:drug effects / Q000378:metabolism
chemical_list: D053583:Interleukin-1beta; D011994:Recombinant Proteins; D051546:Cyclooxygenase 2; D053509:Matrix Metalloproteinase 13; D019278:Matrix Metalloproteinase 3; D020781:Matrix Metalloproteinase 1; D015232:Dinoprostone