title: Mapmi gene contributes to stress tolerance and virulence of the entomopathogenic fungus, Metarhizium acridum.
abstract: Phosphomannose isomerase (PMI) catalyzes the reversible interconversion of fructose 6-phosphate (Fru-6-P) and mannose 6-phosphate (Man-6-P), providing a link between glycolysis and the mannose metabolic pathway. In this study, we identified pmi gene (Mapmi) from the entomopathogenic fungus, Metarhizium acridum, and analyzed its functions using RNA interference (RNAi). Amending the growth medium with cell stress chemicals significantly reduced growth, conidial production and percent germination in Mapmi-RNAi mutant strain, compared to the wild-type strain. Growth of RNAi mutant was lower than the wild type strain with glucose or fructose as sole carbon source. RNAi mutant exhibited a normal growth phenotype with mannose at low concentrations, while trace or high concentration of mannose was more negatively impacted the growth of RNAi mutant than the wild type strain. Infection with Mapmi-RNAi mutant against Locusta migratoria manilensis (Meyen) led to a significantly reduced virulence compared to infection with the wild-type strain. These results suggest that Mapmi plays essential roles in stress tolerance and pathogenicity of M. acridum.
authors.affiliation: Genetic Engineering Research Center, College of Bioengineering, Chongqing University, Chongqing Engineering Research Center for Fungal Insecticides and Key Lab. of Functional Gene and Regulation Technologies under Chongqing Municipal Education Commission, Chongqing 400030, PR China.
authors.forename: Yueqing
authors.initials: Y
authors.lastname: Cao
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.forename: Yuxian
authors.initials: Y
authors.lastname: Xia
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D020224:Expressed Sequence Tags; D005656:Fungal Proteins / Q000037:antagonists & inhibitors / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D015723:Gene Library; D005800:Genes, Fungal; D048188:Locusta migratoria / Q000382:microbiology; D008359:Mannose-6-Phosphate Isomerase / Q000037:antagonists & inhibitors / Q000235:genetics*; D052981:Metarhizium / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology; D034622:RNA Interference; D013172:Spores, Fungal / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D013312:Stress, Physiological; D014774:Virulence / Q000235:genetics
chemical_list: D005656:Fungal Proteins; D008359:Mannose-6-Phosphate Isomerase