title: Photochemically triggered reaction of glucose oxidase in a fused vesicle containing Malachite Green leuconitrile derivative.
abstract: Glucose oxidase (GOD) was encapsulated in vesicles containing a photoionizable Malachite Green leuconitrile derivative (MGL). Subsequent UV irradiation of MGL afforded the fusion of GOD- and glucose-encapsulating vesicles and thus decreased the concentration of glucose in the vesicles. The time dependence of the vesicle fusion was studied using fluorescent probe molecules. This phototriggered fusion could be instrumental in the development of a system for the production of nanometer-sized bioreactors.
authors.affiliation: Department of Chemical Engineering, Nara National College of Technology, Yamato-koriyama, Nara, Japan. ryoko@chem.nara-k.ac.jp
authors.forename: Ryoko M
authors.initials: RM
authors.lastname: Uda
authors.forename: Keisuke
authors.initials: K
authors.lastname: Mori
authors.forename: Takuya
authors.initials: T
authors.lastname: Sugioka
mesh_terms: D017738:Alkanesulfonic Acids / Q000737:chemistry; D019149:Bioreactors; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry; D004339:Drug Compounding / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D005949:Glucose Oxidase / Q000378:metabolism*; D008027:Light; D008561:Membrane Fusion / Q000528:radiation effects*; D008567:Membranes, Artificial; D036103:Nanotechnology / Q000379:methods*; D009281:Naphthalenes / Q000032:analysis; D055668:Photochemical Processes / Q000528:radiation effects; D012394:Rosaniline Dyes / Q000737:chemistry* / Q000378:metabolism; D013050:Spectrometry, Fluorescence
chemical_list: D017738:Alkanesulfonic Acids; D002593:Cetrimonium Compounds; D005456:Fluorescent Dyes; D008567:Membranes, Artificial; D009281:Naphthalenes; D012394:Rosaniline Dyes; C005095:malachite green; C046465:8-amino-1,3,6-naphthalenetrisulfonic acid; C042005:1-octanesulfonic acid; D005949:Glucose Oxidase; D005947:Glucose; D000077286:Cetrimonium