title: Antioxidant, diuretic activities and polyphenol content of Stereospermum kunthianum Cham. (Bignoniaceae).
abstract: Stereospermum kunthianum was used for biological and phytochemical investigations. In biological studies, antioxidant activities were investigated with water, methanol and aqueous acetone extracts. Furthermore, the xanthine oxidase inhibitory activity and the diuretic activity of an aqueous acetone extract were evaluated. In the phytochemical investigations, the flavonoids and polyphenols were quantified spectrophotometrically in all extracts followed by high-performance liquid chromatography-mass spectrometry (HPLC-MS) analysis of an aqueous acetone extract. The 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing/antioxidant power (FRAP) and 2,2'-azinobis (3-ethylbenzoline-6-sulphonate (ABTS) methods have shown that the aqueous acetone extract presents the best antioxidant activities. This aqueous acetone extract was further proven to have interesting xanthine oxidase inhibitory activity, but only a weak diuretic activity. This aqueous acetone extract also possessed the highest phenolic and flavonoid contents. HPLC-MS analysis allowed identifying and quantifying, rutin, isoquercitrin, quercetin, hyperoside, quercitrin and luteolin and the glycosides of ferulic, sinapic p-coumaric acids and kaempferol, apigenin in aqueous-acetone extract.
authors.affiliation: Département de Biochimie-Microbiologie, Université de Ouagadougou, Ouagadougou, Burkina Faso. mcompaore_3@yahoo.fr
authors.forename: M
authors.initials: M
authors.lastname: Compaoré
authors.forename: A
authors.initials: A
authors.lastname: Lamien-Meda
authors.forename: C
authors.initials: C
authors.lastname: Mogoşan
authors.forename: C E
authors.initials: CE
authors.lastname: Lamien
authors.forename: M
authors.initials: M
authors.lastname: Kiendrebeogo
authors.forename: O
authors.initials: O
authors.lastname: Voştinaru
authors.forename: L
authors.initials: L
authors.lastname: Vlase
authors.forename: C
authors.initials: C
authors.lastname: Ionescu
authors.forename: O G
authors.initials: OG
authors.lastname: Nacoulma
mesh_terms: D000096:Acetone; D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D029402:Bignoniaceae / Q000737:chemistry*; D001713:Biphenyl Compounds / Q000037:antagonists & inhibitors; D002851:Chromatography, High Pressure Liquid; D004232:Diuretics / Q000737:chemistry / Q000494:pharmacology*; D005419:Flavonoids / Q000032:analysis; D020128:Inhibitory Concentration 50; D008297:Male; D013058:Mass Spectrometry; D000432:Methanol; D010851:Picrates / Q000037:antagonists & inhibitors; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D059808:Polyphenols / Q000032:analysis; D011188:Potassium / Q000652:urine; D051381:Rats; D017208:Rats, Wistar; D012964:Sodium / Q000652:urine; D013053:Spectrophotometry; D014527:Uric Acid / Q000652:urine; D014867:Water; D014969:Xanthine Oxidase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D004232:Diuretics; D005419:Flavonoids; D010851:Picrates; D010936:Plant Extracts; D059808:Polyphenols; D014867:Water; D000096:Acetone; D014527:Uric Acid; D012964:Sodium; C004931:1,1-diphenyl-2-picrylhydrazyl; D014969:Xanthine Oxidase; D011188:Potassium; D000432:Methanol