title: Identification of various laccases induced by anthracene and contribution to its degradation in a Mediterranean coastal pine litter.
abstract: Mediterranean coastal ecosystems are known to be highly subject to natural and anthropic environmental stress. In this study, we examine the effects of anthracene as a common pollutant on the total microbial communities from a Pinus halepensis litter of a typical Mediterranean coastal site (Les Calanques, Marseille). The main objective was to identify the microbial factors leading the resilience of this ecosystem. Two questions were addressed: (i) how lignin-degrading enzymes (Laccase, Lignin-peroxidase and Mn-peroxidase) are affected by the presence of this molecule, (ii) whether the indigenous consortia are involved in its degradation in mesocosms under favorable incubation conditions (25 °C, 60% WHC) and after different time intervals (1 and 3 month(s)). We found a strong increase in laccase production in the presence of anthracene after 3 months, together with anthracene degradation (28%±5). Moreover 9,10-anthraquinone is detected as the product of anthracene oxidation after 3 months. However neither lignin-peroxidase activity nor Mn-peroxidase activity is detected. Laccase proteins directly extracted from litter were sequenced via Nano-LC-MS/MS and reveal twelve different peptide sequences induced by the presence of anthracene in the mesocoms. Our study confirms the major detoxification role of this enzymatic system and highlights the high degradation potential of fungal species inhabiting P. halepensis litter, a factor in the resilience of Mediterranean ecosystems.
authors.affiliation: Equipe Ecologie Microbienne et Biotechnologies, UMR CNRS IRD 6116, Institut Méditerranéen d'Ecologie et de Paléoécologie, Faculté des Sciences et Techniques de St. Jérôme, Université Paul Cézanne, 13397 Marseille, France. leila_qasemian@yahoo.com
authors.forename: Leila
authors.initials: L
authors.lastname: Qasemian
authors.forename: Daniel
authors.initials: D
authors.lastname: Guiral
authors.forename: Maya
authors.initials: M
authors.lastname: Belghazi
authors.forename: Elisée
authors.initials: E
authors.lastname: Ferré
authors.forename: Raphaël
authors.initials: R
authors.lastname: Gros
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Farnet
mesh_terms: D000873:Anthracenes / Q000032:analysis / Q000378:metabolism*; D000880:Anthraquinones / Q000032:analysis / Q000378:metabolism; D001673:Biodegradation, Environmental; D002851:Chromatography, High Pressure Liquid; D017753:Ecosystem; D042845:Laccase / Q000032:analysis* / Q000145:classification / Q000378:metabolism; D019083:Mediterranean Region; D010544:Peroxidases / Q000032:analysis / Q000378:metabolism; D028223:Pinus; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis / Q000378:metabolism*
chemical_list: D000873:Anthracenes; D000880:Anthraquinones; D012989:Soil Pollutants; C042834:9,10-anthraquinone; D042845:Laccase; D010544:Peroxidases; C042858:lignin peroxidase; C051129:manganese peroxidase; C034020:anthracene