title: CDH4 as a novel putative tumor suppressor gene epigenetically silenced by promoter hypermethylation in nasopharyngeal carcinoma.
abstract: We investigated the transcription levels, promoter methylation status and role as a tumor suppressor gene (TSG) of the cadherin CDH4 in nasopharyngeal carcinoma (NPC). The expression of CDH4 was decreased in NPC cell lines, xenografts and primary tumor biopsies. Promoter hypermethylation of CDH4 was detected in all five NPC cell lines, both NPC xenograft lines and 94.3% of primary tumors but not in any of the 12 normal epithelial samples. Loss of CDH4 expression could be restored by the methyltransferase inhibitor 5-aza-2'-deoxycytidine in NPC cell lines. Ectopic expression of CDH4 in the NPC cell lines inhibits cell proliferation, colony formation, migration and elicit cell communication. CDH4 may be a novel putative TSG that can be frequently and tumor-specifically inactivated by its promoter methylation in NPC.
authors.affiliation: Department of Otolaryngology/Head and Neck Surgery, First Affiliated Hospital of Guangxi Medical University, 22# Shuang Yong Road, Nanning, China.
authors.forename: Chunping
authors.initials: C
authors.lastname: Du
authors.forename: Tingting
authors.initials: T
authors.lastname: Huang
authors.forename: Di
authors.initials: D
authors.lastname: Sun
authors.forename: Yingxi
authors.initials: Y
authors.lastname: Mo
authors.forename: Haiyan
authors.initials: H
authors.lastname: Feng
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Zhou
authors.forename: Xue
authors.initials: X
authors.lastname: Xiao
authors.forename: Nana
authors.initials: N
authors.lastname: Yu
authors.forename: Bo
authors.initials: B
authors.lastname: Hou
authors.forename: Guangwu
authors.initials: G
authors.lastname: Huang
authors.forename: Ingemar
authors.initials: I
authors.lastname: Ernberg
authors.forename: Zhe
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000818:Animals; D015820:Cadherins / Q000235:genetics*; D002277:Carcinoma; D002450:Cell Communication / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D019175:DNA Methylation; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D016147:Genes, Tumor Suppressor; D006801:Humans; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000235:genetics* / Q000473:pathology; D009368:Neoplasm Transplantation; D011401:Promoter Regions, Genetic; D015533:Transcriptional Activation
chemical_list: D015820:Cadherins; C083970:R-cadherin