title: Change in biomechanical parameters after flap creation using the Intralase femtosecond laser and subsequent excimer laser ablation.
abstract: OBJECTIVE
  This study aims to investigate the effect of both flap creation and laser ablation on corneal hysteresis (CH) and corneal resistance factor (CRF), as well as the inward applanation signal amplitude produced by the ocular response analyzer (ORA), immediately following each step of the LASIK procedure using the Intralase femtosecond laser for flap creation.
  
  
  METHODS
  A total of 66 eyes of 35 subjects who underwent Intra-LASIK surgery were prospectively enrolled in this study. Changes in the CH, CRF, and amplitude of the first applanation peak (Peak 1) of the infrared signal were measured preoperatively, immediately after flap elevation and repositioning, as well as subsequent to laser ablation in all eyes. Repeated measures analysis of variance (ANOVA) was performed with a post-test of contrast variable profiles to investigate changes following each step of the procedure.
  
  
  RESULTS
  The repeated measures ANOVA indicated a significant difference (p < 0.001) among the three time points for CH, CRF, and Peak 1. There was no significant change in CH (p = 0.20) or CRF (p = 0.57) after flap creation, but there was significant decrease in these parameters following laser ablation (p < 0.0001), when compared to values obtained pre-operatively. There was a significant decrease in Peak 1 both after flap creation (p < 0.0001) and after subsequent ablation (p < 0.0001), when compared to pre-operative ORA signal peak amplitudes.
  
  
  CONCLUSIONS
  There is a well documented change in corneal biomechanical properties following a LASIK procedure. This study reveals that this change may be predominantly due to laser ablation. However, flap creation with the Intralase laser does produce a biomechanical consequence consistent with reduction of corneal stiffness, as measured by the reduced amplitude of Peak 1. CH and CRF do not fully characterize this change.
authors.affiliation: Kudret Eye Hospital, Ankara, Turkey. aylinclzy@gmail.com
authors.forename: Aylin Kılıç
authors.initials: AK
authors.lastname: Uzbek
authors.forename: Günhal
authors.initials: G
authors.lastname: Kamburoğlu
authors.forename: Ashraf M
authors.initials: AM
authors.lastname: Mahmoud
authors.forename: Cynthia J
authors.initials: CJ
authors.lastname: Roberts
mesh_terms: D000328:Adult; D001251:Astigmatism / Q000503:physiopathology / Q000601:surgery; D001696:Biomechanical Phenomena / Q000502:physiology*; D003315:Cornea / Q000503:physiopathology* / Q000601:surgery; D004108:Dilatation, Pathologic; D004547:Elastic Tissue; D004548:Elasticity / Q000502:physiology*; D005260:Female; D006801:Humans; D020731:Keratomileusis, Laser In Situ; D054018:Lasers, Excimer / Q000627:therapeutic use*; D008297:Male; D009216:Myopia / Q000503:physiopathology* / Q000601:surgery; D011446:Prospective Studies; D013524:Surgical Flaps