title: Lactic acid bacteria in the inhibition of Fusarium graminearum and deoxynivalenol detoxification.
abstract: OBJECTIVE
  Considering the agronomic and industrial damage that is caused by the fungus Fusarium graminearum, as well as the serious health risks it poses to humans and animals exposed to F. graminearum-produced mycotoxin deoxynivalenol (DON), this study evaluated the ability of different lactic acid bacteria (LAB) strains to inhibit fungal development and remove DON in vitro.
  
  
  RESULTS
  The antagonistic effects of strains and commercial cultures of LAB were evaluated against F. graminearum IAPAR 2218 by the agar diffusion method. Additionally, the influence of the culture media, pH and the presence of lactic and acetic acid on these effects was tested. The capacity to remove DON by viable cells and heat-inactivated cells was analysed in liquid media and quantified by high performance liquid chromatography (HPLC). All isolated strains and commercial cultures inhibited the fungus and removed DON. The pH and culture media concentration did not influence these abilities, but heat inactivation had a strong effect on the ability of bacteria to remove mycotoxin.
  
  
  CONCLUSIONS
  The isolated bacteria are able to inhibit F. graminearum growth and remove DON in vitro.
  
  
  CONCLUSIONS
  This study suggests potential application of the isolated LAB strains in the inhibition of F. graminearum IAPAR 2218 and DON removal in vitro.
authors.affiliation: Department of Food Science and Technology, State University of Londrina, Curitiba-Paraná, Brazil. talitaszlapak@ibest.com.br
authors.forename: T S
authors.initials: TS
authors.lastname: Franco
authors.forename: S
authors.initials: S
authors.lastname: Garcia
authors.forename: E Y
authors.initials: EY
authors.lastname: Hirooka
authors.forename: Y S
authors.initials: YS
authors.lastname: Ono
authors.forename: J S
authors.initials: JS
authors.lastname: dos Santos
mesh_terms: D019342:Acetic Acid / Q000737:chemistry; D000898:Antibiosis; D061046:Biological Control Agents; D003470:Culture Media / Q000737:chemistry; D005670:Fusarium / Q000254:growth & development* / Q000378:metabolism; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid / Q000737:chemistry; D007777:Lactobacillaceae / Q000502:physiology*; D014255:Trichothecenes / Q000378:metabolism*; D014908:Triticum / Q000382:microbiology
chemical_list: D061046:Biological Control Agents; D003470:Culture Media; D014255:Trichothecenes; D019344:Lactic Acid; C007262:deoxynivalenol; D019342:Acetic Acid