title: Plaunotol induces a comparative increase of acidic mucin fraction in gastric juice.
abstract: OBJECTIVE
  Gastric mucus protects the gastric mucosa. Plaunotol, a gastroprotective agent, has been shown to increase mucus production in animal models. However, it is unclear whether plaunotol benefits human gastric mucus secretion.
  
  
  METHODS
  Twenty-five patients with atrophic gastritis were studied. All patients underwent gastroendoscopy and gastric juice was collected before and after plaunotol treatment for 3 months. Gastric juice mucin was examined by gel filtration as well as anion-exchange chromatography. The identification of each fraction was examined by enzyme-linked immunosorbent assay (ELISA) with the use of HGM75 and HIK1083, antibodies against mucin from surface mucus cells and from gastric glandular mucus cells, respectively.
  
  
  RESULTS
  Plaunotol significantly increased the total gastric juice volume (7.8mL before vs. 10.7mL, after administration; p=0.03). By anion exchange chromatography, we detected three mucin fractions (Fr I-III). Fr I strongly reacted with HGM75 but did not react with HIK1083. The other fractions (Fr II, III) reacted with HIK1083 but weakly reacted with HGM75. After administration of plaunotol, a significant increase in Fr III (acidic mucin) was observed (p=0.02).
  
  
  CONCLUSIONS
  Long-term administration of plaunotol changes the composition of gastric juice mucin, including a significant increase in the proportion of acidic mucin fraction.
authors.affiliation: Department of Medicine and Molecular Science, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan.
authors.forename: Masana
authors.initials: M
authors.lastname: Tatsugami
authors.forename: Masanori
authors.initials: M
authors.lastname: Ito
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Ishihara
authors.forename: Ken
authors.initials: K
authors.lastname: Haruma
authors.forename: Masaharu
authors.initials: M
authors.lastname: Sumii
authors.forename: Atsunori
authors.initials: A
authors.lastname: Sasaki
authors.forename: Shosuke
authors.initials: S
authors.lastname: Kitamura
authors.forename: Akemi
authors.initials: A
authors.lastname: Takamura
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Wada
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Matsumoto
authors.forename: Shinobu
authors.initials: S
authors.lastname: Imagawa
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Takata
authors.forename: Shinji
authors.initials: S
authors.lastname: Tanaka
authors.forename: Masaharu
authors.initials: M
authors.lastname: Yoshihara
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Chayama
mesh_terms: D000368:Aged; D000890:Anti-Infective Agents / Q000494:pharmacology*; D004224:Diterpenes; D004797:Enzyme-Linked Immunosorbent Assay; D005233:Fatty Alcohols / Q000494:pharmacology*; D005260:Female; D005750:Gastric Juice / Q000737:chemistry / Q000187:drug effects*; D005752:Gastric Mucins / Q000032:analysis*; D005755:Gastrins / Q000097:blood; D016480:Helicobacter pylori / Q000187:drug effects; D006801:Humans; D008297:Male; D020116:Pepsinogen A / Q000097:blood
chemical_list: D000890:Anti-Infective Agents; D004224:Diterpenes; D005233:Fatty Alcohols; D005752:Gastric Mucins; D005755:Gastrins; D020116:Pepsinogen A; C047686:plaunotol