title: Analysis of H19 methylation in control and abnormal human embryos, sperm and oocytes.
abstract: ART is suspected to generate increased imprinting errors in the lineage. Following an intra cytoplasmic sperm injection (ICSI) procedure, a certain number of embryos fail to develop normally and imprinting disorders may be associated to the developmental failure. To evaluate this hypothesis, we analysed the methylation profile of H19DMR, a paternally imprinting control region, in high-graded blastocysts, in embryos showing developmental anomalies, in the matching sperm and in oocytes of the concerned couples when they were available. Significant hypomethylation of the paternal allele was observed in half of the embryos, independently of the stage at which they were arrested (morula, compacted morula, pre blastocyst or BC-graded blastocysts). Conversely, some embryos showed significant methylation on the maternal allele, whereas few others showed both hypomethylation of the paternal allele and abnormal methylation of the maternal allele. The matching sperm at the origin of the embryos exhibited normal methylated H19 patterns. Thus, hypomethylation of the paternal allele in the embryos does not seem inherited from the sperm but likely reflects instability of the imprint during the demethylating process, which occurred in the early embryo. Analysis of a few oocytes suggests that the defect in erasure of the paternal imprint in the maternal germ line may be responsible for the residual methylation of the maternal allele in some embryos. None of these imprinting alterations could be related to a particular stage of developmental arrest; compared with high-grade blastocysts, embryos with developmental failure are more likely to have abnormal imprinting at H19 (P<0.05).
authors.affiliation: INSERM U846, Institut Cellule Souche et Cerveau, 18 Av Doyen Lépine,Bron, France.
authors.forename: Samira
authors.initials: S
authors.lastname: Ibala-Romdhane
authors.forename: Mohamed
authors.initials: M
authors.lastname: Al-Khtib
authors.forename: Rita
authors.initials: R
authors.lastname: Khoueiry
authors.forename: Thierry
authors.initials: T
authors.lastname: Blachère
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Guérin
authors.forename: Annick
authors.initials: A
authors.lastname: Lefèvre
mesh_terms: D000483:Alleles; D001483:Base Sequence; D001755:Blastocyst / Q000378:metabolism; D047108:Embryonic Development / Q000235:genetics*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D018392:Genomic Imprinting; D006801:Humans; D008297:Male; D008745:Methylation; D008969:Molecular Sequence Data; D009865:Oocytes / Q000378:metabolism*; D020641:Polymorphism, Single Nucleotide; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated / Q000378:metabolism*; D013094:Spermatozoa / Q000378:metabolism*
chemical_list: C094335:H19 long non-coding RNA; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated