title: A sequential strand-displacement strategy enables efficient six-step DNA-templated synthesis.
abstract: We developed a sequential strand-displacement strategy for multistep DNA-templated synthesis (DTS) and used it to mediate an efficient six-step DTS that proceeded in 35% overall yield (83% average yield per step). The efficiency of this approach and the fact that the final product remains linked to a DNA sequence that fully encodes its reaction history suggests its utility for the translation of DNA sequences into high-complexity synthetic libraries suitable for in vitro selection.
authors.affiliation: Howard Hughes Medical Institute, Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA.
authors.forename: Yu
authors.initials: Y
authors.lastname: He
authors.forename: David R
authors.initials: DR
authors.lastname: Liu
mesh_terms: D060326:Chemistry Techniques, Synthetic / Q000379:methods*; D004247:DNA / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D054852:Small Molecule Libraries / Q000138:chemical synthesis / Q000737:chemistry
chemical_list: D054852:Small Molecule Libraries; D004247:DNA