title: OCT4B1, a novel spliced variant of OCT4, generates a stable truncated protein with a potential role in stress response.
abstract: A novel variant of OCT4, OCT4B1, is highly expressed in pluripotent cells. OCT4B1 is also upregulated in various tumors and cell lines. Here, we have constructed a HA-tagged OCT4B1 construct, which could be translated into an N-terminally tagged protein, detectable by HA antibody. Western blotting revealed that OCT4B1 can act as a precursor for OCT4B, and it can also produce a truncated protein with a molecular size corresponding to that of predicted OCT4B1. Furthermore, our data demonstrated a cytoplasmic distribution for both isoforms. Interestingly, the expression ratio of OCT4B1/OCT4B transcripts and proteins was significantly elevated under the heat-stress condition.
authors.affiliation: Molecular Genetics Department, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Ehsan
authors.initials: E
authors.lastname: Farashahi Yazd
authors.forename: Mahmood Reza
authors.initials: MR
authors.lastname: Rafiee
authors.forename: Masoud
authors.initials: M
authors.lastname: Soleimani
authors.forename: Mahmood
authors.initials: M
authors.lastname: Tavallaei
authors.forename: Maryam Kabir
authors.initials: MK
authors.lastname: Salmani
authors.forename: Seyed Javad
authors.initials: SJ
authors.lastname: Mowla
mesh_terms: D017398:Alternative Splicing; D015415:Biomarkers; D015153:Blotting, Western; D002460:Cell Line; D057809:HEK293 Cells; D018869:Heat-Shock Response; D006801:Humans; D007150:Immunohistochemistry; D050814:Octamer Transcription Factor-3 / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D039904:Pluripotent Stem Cells / Q000166:cytology; D020033:Protein Isoforms; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013312:Stress, Physiological
chemical_list: D015415:Biomarkers; D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human; D020033:Protein Isoforms; D012333:RNA, Messenger