title: Lentibacter algarum gen. nov., sp. nov., isolated from coastal water during a massive green algae bloom.
abstract: Two novel Gram-stain-negative, aerobic strains (ZXM100(T) and ZXM098) were isolated from seawater in the coastal region of Qingdao (36.027° N 120.184° E), China, during a massive green algae bloom. Cells were ovoid to irregular short rods and lacked flagella. Poly-β-hydroxybutyrate was accumulated. Cells did not contain bacteriochlorophyll a. The isolates grew at NaCl concentrations of 3-9 % and 22-28 °C. C(18 : 1)ω7c, C(18 : 0) and C(16 : 0) were the major fatty acid components. The polar lipids of ZXM100(T) were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, a single lipid and an unidentified aminolipid. The predominant isoprenoid quinone of ZXM100(T) was Q-10. Phylogenetic analysis based on 16S rRNA gene sequences showed that strains belonged to the family Rhodobacteraceae. Based on the results obtained in this study, strain ZXM100(T) was considered to represent a novel species in a new genus, for which the name Lentibacter algarum gen. nov., sp. nov. is proposed. The type strain is ZXM100(T) ( = LMG 24861(T) = CGMCC 1.10234(T)); the DNA G+C content of the type strain is 54.6 mol%.
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao, PR China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Li
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao, PR China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Qu
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao, PR China.
authors.forename: Xiuming
authors.initials: X
authors.lastname: Zhang
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao, PR China.
authors.forename: Xiao-Hua
authors.initials: XH
authors.lastname: Zhang
mesh_terms: D000332:Aerobiosis; D015373:Bacterial Typing Techniques; D002681:China; D000460:Chlorophyta / Q000254:growth & development*; D016000:Cluster Analysis; D003600:Cytosol / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005068:Eutrophication; D005227:Fatty Acids / Q000032:analysis; D006885:Hydroxybutyrates / Q000032:analysis; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011091:Polyesters / Q000032:analysis; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D041902:Rhodobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D000094122:Polyhydroxybutyrates
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D006885:Hydroxybutyrates; D010743:Phospholipids; D011091:Polyesters; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates