title: Differential dependency of human cancer cells on vascular endothelial growth factor-mediated autocrine growth and survival.
abstract: Analysis using the public microarray database Gene Expression Omnibus indicates significantly higher mRNA expression of VEGF and VEGFRs in colorectal cancer and high grade astrocytoma but not in hepatocellular carcinoma compared to normal tissue. Human malignant astrocytoma cell lines (U251-MG and U373-MG) and HT-1080 fibrosarcoma cells expressed relatively higher levels of VEGF and VEGFRs compared to hepatocellular and colorectal cancer cell lines. Administration of exogenous VEGF-A induced cell growth in a dose-dependent fashion in astrocytoma and fibrosarcoma cells but not in colorectal and hepatocellular cancer cells. The blockade of VEGF inhibited cell survival only in U251-MG, U373-MG and HT-1080 cells. These results collectively suggest the role of autocrine VEGF signaling in various cancer cells and provide a basis for the variable clinical responses to antiangiogenic therapy observed in different types of malignancies.
authors.affiliation: Department of Bio and Brain Engineering, KAIST, Daejeon 305-701, Republic of Korea.
authors.forename: Jungwhoi
authors.initials: J
authors.lastname: Lee
authors.forename: Jungsul
authors.initials: J
authors.lastname: Lee
authors.forename: Hana
authors.initials: H
authors.lastname: Yu
authors.forename: Kyungsun
authors.initials: K
authors.lastname: Choi
authors.forename: Chulhee
authors.initials: C
authors.lastname: Choi
mesh_terms: D001254:Astrocytoma / Q000378:metabolism / Q000473:pathology; D019898:Autocrine Communication; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival; D015179:Colorectal Neoplasms / Q000378:metabolism / Q000473:pathology; D004797:Enzyme-Linked Immunosorbent Assay; D005354:Fibrosarcoma / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology; D046228:Microarray Analysis; D009369:Neoplasms / Q000378:metabolism* / Q000473:pathology*; D018919:Neovascularization, Physiologic; D034622:RNA Interference; D012333:RNA, Messenger / Q000096:biosynthesis; D034741:RNA, Small Interfering; D040262:Receptors, Vascular Endothelial Growth Factor / Q000096:biosynthesis / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D042442:Vascular Endothelial Growth Factors / Q000096:biosynthesis / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D034741:RNA, Small Interfering; D011994:Recombinant Proteins; D042442:Vascular Endothelial Growth Factors; D040262:Receptors, Vascular Endothelial Growth Factor