title: Methods for testing platelet function for transfusion medicine.
abstract: BACKGROUND
  Currently only indirect measures are required for monitoring the function of platelets in platelet concentrates (PC).
  
  
  METHODS
  This is an overview on currently available commercialized methods that have been used to determine platelet function in donors, concentrates and after transfusion. We show examples for the application of the no/low shear methods light-transmission aggregometry, flow cytometry, multiple electrode aggregometry, thrombelastography and dynamic light scattering, and those applying high shear, the platelet function analyzer-100, and the cone and plate analyzer. Advantages and disadvantages of the various methods to screen donors, evaluate the haemostatic properties maintained in the PC and after transfusion are discussed, based on considerations of platelet physiology, and the feasibility of the various procedures. This survey focuses on reports from the last 10 years, as the technology for the production of PCs has advanced significantly during the last few years.
  
  
  CONCLUSIONS
  Specific aspects of platelet function can be assessed by the no/low shear methods, while the high shear methods provide more general analysis of platelet haemostatic competence. Yet, there is no strong evidence that the in vitro data correspond with the clinical outcome.
authors.affiliation: Department for Blood Group Serology and Transfusion Medicine, Medical University Vienna, Vienna, Austria. simon.panzer@meduniwien.ac.at
authors.forename: S
authors.initials: S
authors.lastname: Panzer
authors.forename: P
authors.initials: P
authors.lastname: Jilma
mesh_terms: D001760:Bleeding Time / Q000295:instrumentation*; D001792:Blood Platelets / Q000502:physiology; D004566:Electrodes; D019544:Equipment Failure Analysis; D005434:Flow Cytometry / Q000379:methods*; D006487:Hemostasis; D006801:Humans; D010976:Platelet Count / Q000379:methods; D010979:Platelet Function Tests / Q000379:methods*; D017713:Platelet Transfusion / Q000379:methods*; D013916:Thrombelastography / Q000379:methods*