title: Analysis of the salivary proteome in gingivitis patients.
abstract: OBJECTIVE
  Gingivitis is a disease that is characterized by inflammation of the gingival tissue, which can progress to periodontitis and tooth loss. Although many studies have attempted to identify salivary proteins that are associated with the disease, this is the first study to use a proteomic approach to analyze and compare the proteomic profile of whole saliva from gingivitis patients and healthy controls.
  
  
  METHODS
  To analyze the saliva proteome, two-dimensional gel electrophoresis and liquid chromatography were used, followed by mass spectrometry.
  
  
  RESULTS
  The analyses showed that gingival inflammation was associated with increased amounts of blood proteins (serum albumin and hemoglobin), immunoglobulin peptides and keratins. In the control group, salivary cystatins, which were detected using capillary Liquid Chromatography on line to electrospray ionization Quadrupole Time-of-flight mass spectrometry, appeared to be more abundant.
  
  
  CONCLUSIONS
  This approach provides novel insight into profiles of the salivary proteome during gingival inflammation, which may contribute to improvements in diagnosis.
authors.affiliation: Laboratório de Genética Aplicada, Instituto Nacional de Câncer, INCA, Rio de Janeiro, Brasil. lorena.da@terra.com.br
authors.forename: L da R
authors.initials: Lda R
authors.lastname: Gonçalves
authors.forename: M R
authors.initials: MR
authors.lastname: Soares
authors.forename: F C S
authors.initials: FC
authors.lastname: Nogueira
authors.forename: C H S
authors.initials: CH
authors.lastname: Garcia
authors.forename: D R
authors.initials: DR
authors.lastname: Camisasca
authors.forename: G
authors.initials: G
authors.lastname: Domont
authors.forename: A C R
authors.initials: AC
authors.lastname: Feitosa
authors.forename: D A
authors.initials: DA
authors.lastname: Pereira
authors.forename: R B
authors.initials: RB
authors.lastname: Zingali
authors.forename: G
authors.initials: G
authors.lastname: Alves
mesh_terms: D000328:Adult; D002853:Chromatography, Liquid; D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D020869:Gene Expression Profiling; D005891:Gingivitis / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D020543:Proteome; D012471:Salivary Proteins and Peptides / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D020543:Proteome; D012471:Salivary Proteins and Peptides