title: Evaluation of soybean methanol fraction on acute inflammation.
abstract: Soybeans have been of interest of researchers because of the presence of isoflavones, a subclass of flavonoids, which have demonstrated anti-inflammatory activity. The aim of this study was investigate the anti-inflammatory activity of the methanol fraction from soybean, which contains mainly isoflavone glucosides and malonylglucosides. The anti-inflammatory activity of the methanol fraction from soybean was studied using croton oil-induced mouse ear edema and carrageenan-induced pleurisy models. The methanol fraction inhibited the ear edema in a dose-dependent manner: 0.625 mg/kg by 44.23% (P<.05), 1.25 mg/kg by 60.68% (P<.01), and 2.5 mg/kg by 65.68% (P<.01). Myeloperoxidase enzyme activity was reduced at the dose of 2.5 mg/kg (64.79%, P<.05). No effects were seen on carrageenan-induced pleurisy at different doses of the methanol fraction (100 or 400 mg/kg). These results demonstrated that the methanol fraction containing conjugated isoflavones showed topical anti-inflammatory activity. There was no acute toxicity in Swiss mice after oral administration of the fraction, at doses of 1,000, 2,000, 3,000, and 4,000 mg/kg.
authors.affiliation: Department of Pharmacy and Pharmacology, State University of Maringá, Maringá, Paraná, Brazil.
authors.forename: Vanessa da Silva
authors.initials: Vda S
authors.lastname: Carrara
authors.forename: Ciomar Bersani
authors.initials: CB
authors.lastname: Amado
authors.forename: Juliana Oliveira
authors.initials: JO
authors.lastname: de Melo
authors.forename: José Marcos Gontijo
authors.initials: JM
authors.lastname: Mandarino
authors.forename: Diógenes Aparício Garcia
authors.initials: DA
authors.lastname: Cortez
mesh_terms: D000208:Acute Disease; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002351:Carrageenan / Q000009:adverse effects; D003436:Croton Oil / Q000009:adverse effects; D004353:Drug Evaluation, Preclinical; D004423:Ear / Q000473:pathology; D004487:Edema / Q000139:chemically induced; D005419:Flavonoids / Q000494:pharmacology; D007249:Inflammation / Q000473:pathology*; D008297:Male; D000432:Methanol; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology*; D010998:Pleurisy / Q000139:chemically induced / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D013025:Glycine max / Q000737:chemistry*
chemical_list: D000893:Anti-Inflammatory Agents; D005419:Flavonoids; D010936:Plant Extracts; D003436:Croton Oil; D002351:Carrageenan; D000432:Methanol