title: 2D format for screening bacterial cells at the throughput of flow cytometry.
abstract: We present a method for generating gel-based unordered 2D arrays of bacterial cells of a very high density, up to 10(5) cells per mm(2). Bacteria in a suspension are focused into a thin layer when the suspension and a dry gel matrix penetrate each other. Formation of a second gel from gel-forming components contained in the suspension results in immobilization of the cells. The immobilized cells stay alive and can repeatedly divide to produce microcolonies. The method provides for high-throughput screening and massively parallel analysis of individual cells in large populations, as well as for rapid isolation of rare clones.
authors.affiliation: Institute of Protein Research of the Russian Academy of Sciences, 4 Institutskaya Str., Pushchino, Moscow Region 142290, Russia.
authors.forename: Alexander A
authors.initials: AA
authors.lastname: Gordeev
authors.forename: Timur R
authors.initials: TR
authors.lastname: Samatov
authors.forename: Helena V
authors.initials: HV
authors.lastname: Chetverina
authors.forename: Alexander B
authors.initials: AB
authors.lastname: Chetverin
mesh_terms: D000180:Acrylic Resins; D001431:Bacteriological Techniques / Q000379:methods*; D001709:Biotechnology / Q000379:methods*; D018914:Cells, Immobilized; D004926:Escherichia coli / Q000166:cytology* / Q000502:physiology; D008403:Mass Screening / Q000379:methods*; D008853:Microscopy / Q000379:methods
chemical_list: D000180:Acrylic Resins; C016680:polyacrylamide gels