title: Molecular cloning and characterization of an F-box family gene CarF-box1 from chickpea (Cicer arietinum L.).
abstract: F-box protein family has been found to play important roles in plant development and abiotic stress responses via the ubiquitin pathway. In this study, an F-box gene CarF-box1 (for Cicer arietinum F-box gene 1, Genbank accession no. GU247510) was isolated based on a cDNA library constructed with chickpea seedling leaves treated by polyethylene glycol. CarF-box1 encoded a putative protein with 345 amino acids and contained no intron within genomic DNA sequence. CarF-box1 is a KFB-type F-box protein, having a conserved F-box domain in the N-terminus and a Kelch repeat domain in the C-terminus. CarF-box1 was localized in the nucleus. CarF-box1 exhibited organ-specific expression and showed different expression patterns during seed development and germination processes, especially strongly expressed in the blooming flowers. In the leaves, CarF-box1 could be significantly induced by drought stress and slightly induced by IAA treatment, while in the roots, CarF-box1 could be strongly induced by drought, salinity and methyl jasmonate stresses. Our results suggest that CarF-box1 encodes an F-box protein and may be involved in various plant developmental processes and abiotic stress responses.
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Yuying
authors.initials: Y
authors.lastname: Jia
authors.forename: Hanyan
authors.initials: H
authors.lastname: Gu
authors.forename: Xiansheng
authors.initials: X
authors.lastname: Wang
authors.forename: Quanjia
authors.initials: Q
authors.lastname: Chen
authors.forename: Shubing
authors.initials: S
authors.lastname: Shi
authors.forename: Jusong
authors.initials: J
authors.lastname: Zhang
authors.forename: Lin
authors.initials: L
authors.lastname: Ma
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
authors.forename: Hao
authors.initials: H
authors.lastname: Ma
mesh_terms: D000085:Acetates / Q000494:pharmacology; D001483:Base Sequence; D029863:Cicer / Q000235:genetics* / Q000378:metabolism; D016000:Cluster Analysis; D019295:Computational Biology; D003517:Cyclopentanes / Q000494:pharmacology; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D055864:Droughts; D044783:F-Box Proteins / Q000235:genetics* / Q000378:metabolism*; D035264:Flowers / Q000378:metabolism; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects / Q000502:physiology*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000502:physiology*; D008969:Molecular Sequence Data; D054883:Oxylipins / Q000494:pharmacology; D010802:Phylogeny; D018515:Plant Leaves / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism; D054712:Salinity; D036226:Seedlings / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D017931:DNA Primers; D018076:DNA, Complementary; D044783:F-Box Proteins; D054883:Oxylipins; C072239:methyl jasmonate