title: A novel salt-inducible gene SbSI-1 from Salicornia brachiata confers salt and desiccation tolerance in E. coli.
abstract: Salicornia brachiata is one of the extreme salt tolerant plants and grows luxuriantly in coastal areas. Previously we have reported isolation and characterization of ESTs from S. brachiata with large number of unknown gene sequences. Reverse Northern analysis showed upregulation and downregulation of few unknown genes in response to salinity. Some of these unknown genes were made full length and their functional analysis is being tested. In this study, we have selected a novel unknown salt inducible gene SbSI-1 (Salicornia brachiata salt inducible-1) for the functional validation. The SbSI-1 (Gen-Bank accession number JF 965339) was made full length and characterized in detail for its functional validation under desiccation and salinity. The SbSI-1 gene is 917 bp long, and contained 437 bp 3' UTR, and 480 bp ORF region encoding 159 amino acids protein with estimated molecular mass of 18.39 kDa and pI 8.58. The real time PCR analysis revealed high transcript expression in salt, desiccation, cold and heat stresses. However, the maximum expression was obtained by desiccation. The ORF region of SbSI-1 was cloned in pET28a vector and transformed in BL21 (DE3) E. coli cells. The SbSI-1 recombinant E. coli cells showed tolerance to desiccation and salinity stress compared to only vector in the presence of stress.
authors.affiliation: Discipline of Marine Biotechnology and Ecology, Central Salt and Marine Chemicals Research Institute (Council of Scientific and Industrial Research), G. B. Marg, Bhavnagar 364 021, Gujarat, India.
authors.forename: Narendra Singh
authors.initials: NS
authors.lastname: Yadav
authors.forename: Deo
authors.initials: D
authors.lastname: Rashmi
authors.forename: Dinkar
authors.initials: D
authors.lastname: Singh
authors.forename: Pradeep K
authors.initials: PK
authors.lastname: Agarwal
authors.forename: Bhavanath
authors.initials: B
authors.lastname: Jha
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics*; D015152:Blotting, Northern; D019663:Chenopodiaceae / Q000235:genetics*; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D003681:Dehydration; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli; D017343:Genes, Plant / Q000235:genetics*; D005822:Genetic Vectors; D016366:Open Reading Frames / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D054712:Salinity; D013312:Stress, Physiological / Q000235:genetics*; D014169:Transformation, Bacterial
chemical_list: D017931:DNA Primers