title: Non-parametric change-point method for differential gene expression detection.
abstract: BACKGROUND
  We proposed a non-parametric method, named Non-Parametric Change Point Statistic (NPCPS for short), by using a single equation for detecting differential gene expression (DGE) in microarray data. NPCPS is based on the change point theory to provide effective DGE detecting ability.
  
  
  METHODS
  NPCPS used the data distribution of the normal samples as input, and detects DGE in the cancer samples by locating the change point of gene expression profile. An estimate of the change point position generated by NPCPS enables the identification of the samples containing DGE. Monte Carlo simulation and ROC study were applied to examine the detecting accuracy of NPCPS, and the experiment on real microarray data of breast cancer was carried out to compare NPCPS with other methods.
  
  
  CONCLUSIONS
  Simulation study indicated that NPCPS was more effective for detecting DGE in cancer subset compared with five parametric methods and one non-parametric method. When there were more than 8 cancer samples containing DGE, the type I error of NPCPS was below 0.01. Experiment results showed both good accuracy and reliability of NPCPS. Out of the 30 top genes ranked by using NPCPS, 16 genes were reported as relevant to cancer. Correlations between the detecting result of NPCPS and the compared methods were less than 0.05, while between the other methods the values were from 0.20 to 0.84. This indicates that NPCPS is working on different features and thus provides DGE identification from a distinct perspective comparing with the other mean or median based methods.
authors.affiliation: Key Laboratory for Symbol Computation and Knowledge Engineering of National Education Ministry, College of Computer Science and Technology, Jilin University, Jilin, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Wang
authors.forename: Chunguo
authors.initials: C
authors.lastname: Wu
authors.forename: Zhaohua
authors.initials: Z
authors.lastname: Ji
authors.forename: Binghong
authors.initials: B
authors.lastname: Wang
authors.forename: Yanchun
authors.initials: Y
authors.lastname: Liang
mesh_terms: D015870:Gene Expression / Q000235:genetics*; D020869:Gene Expression Profiling / Q000379:methods; D015233:Models, Statistical; D009010:Monte Carlo Method; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D018709:Statistics, Nonparametric