title: Application of multiwall carbon nanotubes-based matrix solid phase dispersion extraction for determination of hormones in butter by gas chromatography mass spectrometry.
abstract: The multiwall carbon nanotubes (MWCNTs)-based matrix solid phase dispersion (MSPD) was applied for the extraction of hormones, including 17-α-ethinylestradiol, 17-α-estradiol, estriol, 17-β-estradiol, estrone, medroxyprogesterone, progesterone and norethisterone acetate in butter samples. The method includes MSPD extraction of the target analytes from butter samples, derivatization of hormones with heptafluorobutyric acid anhydride-acetonitrile mixture, and determination by gas chromatography-mass spectrometry. The mixture containing 0.30 g graphitized MWCNTs and 0.10 g MWCNTs was selected as absorbent. Ethyl acetate was used as elution solvent. The elution solvent volume and flow rate were 12 mL and 0.9 mL min(-1), respectively. The recoveries of hormones obtained by analyzing the five spiked butter samples were from 84.5 to 111.2% and relative standard deviations from 1.9 to 8.9%. Limits of detection and quantification for determining the analytes were in the range of 0.2-1.3 and 0.8-4.5 μg kg(-1), respectively. Compared with other traditional methods, the proposed method is simpler in the operation and shorter in the sample pretreatment time.
authors.affiliation: College of Chemistry, Jilin University, 2699 Qianjin Street, Changchun 130012, China. surui09@mails.jlu.edu.cn
authors.forename: Rui
authors.initials: R
authors.lastname: Su
authors.forename: Xinghua
authors.initials: X
authors.lastname: Wang
authors.forename: Xu
authors.initials: X
authors.lastname: Xu
authors.forename: Ziming
authors.initials: Z
authors.lastname: Wang
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.forename: Xin
authors.initials: X
authors.lastname: Zhao
authors.forename: Xueyuan
authors.initials: X
authors.lastname: Li
authors.forename: Hanqi
authors.initials: H
authors.lastname: Zhang
authors.forename: Aimin
authors.initials: A
authors.lastname: Yu
mesh_terms: D000327:Adsorption; D002079:Butter / Q000032:analysis*; D005506:Food Contamination; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006728:Hormones / Q000032:analysis* / Q000302:isolation & purification*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods*
chemical_list: D006728:Hormones; D037742:Nanotubes, Carbon; D002079:Butter