title: Pharmacokinetics of ergosterol in rats using rapid resolution liquid chromatography-atmospheric pressure chemical ionization multi-stage tandem mass spectrometry and rapid resolution liquid chromatography/tandem mass spectrometry.
abstract: Rapid resolution liquid chromatography/tandem multi-stage mass spectrometry (RRLC-MS(n)) and rapid resolution liquid chromatography/tandem mass spectrometry (RRLC/MS/MS) methods were developed for the identification and quantification of ergosterol and its metabolites from rat plasma, urine and faeces. Two metabolites (ERG1 and ERG2) were identified by RRLC/MS(n). The concentrations of the ergosterol were determined by RRLC/MS/MS. The separation was performed on an Agilent Zorbax SB-C18 with the mobile phase consisting of methanol and water (containing 0.1% formic acid). The detection was carried out by means of atmospheric pressure chemical ionization mass spectrometry in positive ion mode with multiple reaction monitoring (MRM). Linear calibration curves were obtained in the concentration range of 7-2000, 6-2000 and 8-7500 ng/mL for plasma, urine and faecal homogenate, respectively. The intra- and inter-day precision values (RSD) were below 10%. The method was applied to the pharmacokinetic properties and elimination pathway of ergosterol in rats.
authors.affiliation: Biomedicine Key Laboratory of Shaanxi Province, The School of Life Sciences, Northwest University, Xi'an, Shaanxi 710069, China. zyy@nwu.edu.cn
authors.forename: Ying-Yong
authors.initials: YY
authors.lastname: Zhao
authors.forename: Xian-Long
authors.initials: XL
authors.lastname: Cheng
authors.forename: Rui
authors.initials: R
authors.lastname: Liu
authors.forename: Charlene C
authors.initials: CC
authors.lastname: Ho
authors.forename: Feng
authors.initials: F
authors.lastname: Wei
authors.forename: Sui-Hong
authors.initials: SH
authors.lastname: Yan
authors.forename: Rui-Chao
authors.initials: RC
authors.lastname: Lin
authors.forename: Yongmin
authors.initials: Y
authors.lastname: Zhang
authors.forename: Wen-Ji
authors.initials: WJ
authors.lastname: Sun
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D004355:Drug Stability; D004875:Ergosterol / Q000032:analysis / Q000493:pharmacokinetics* / Q000652:urine; D005243:Feces / Q000737:chemistry; D016018:Least-Squares Analysis; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004875:Ergosterol