title: PCR study of a series of ASCUS cases HPV-positive by HCII.
abstract: Most guidelines currently recommend the testing of human papillomavirus (HPV) in ASCUS cases. The most used method for this purpose is Hybrid Capture II (HCII), but PCR techniques with GP5+/6+ primers can be also applied. Furthermore, the HCII high-risk probe test for detection of HPV shows cross-reactivity with low-risk HPV. Although this cross-reactivity has been studied in screening populations, it has received little attention in ASCUS cases. To compare the performance of the HCII high-risk probe test and PCR for the detection of HPV in ASCUS cases. We randomly selected 83 ASCUS cases that were positive for high-risk HPV by HCII and applied the PCR test using MYO9-11 and GP5+/6+ primers to samples from these cases. Our results show cross-reactivity with low-risk HPV in 25.3% (21/83) of the HCII+ PCR+ cases. Regarding the follow-up our results emphasize the importance of HPV typing, especially for HPV 16 infection. We propose the use of PCR techniques using GP5+/6+ consensus primers for the screening of HPV in ASCUS.
authors.affiliation: Department of Pathology, Hospital del Mar, Autonomous University of Barcelona, Barcelona, Catalunya, Spain. 86780@parcdesalutmar.cat
authors.forename: Francesc
authors.initials: F
authors.lastname: Alameda
authors.forename: Beatriz
authors.initials: B
authors.lastname: Bellosillo
authors.forename: Belén
authors.initials: B
authors.lastname: Lloveras
authors.forename: Silvia
authors.initials: S
authors.lastname: Pairet
authors.forename: Mercè
authors.initials: M
authors.lastname: Musset
authors.forename: Lara
authors.initials: L
authors.lastname: Pijuan
authors.forename: Lluisa
authors.initials: L
authors.lastname: Mariñoso
authors.forename: Gemma
authors.initials: G
authors.lastname: Mancebo
authors.forename: Fernando
authors.initials: F
authors.lastname: Larrazabal
authors.forename: Ramón
authors.initials: R
authors.lastname: Carreras
authors.forename: Sergi
authors.initials: S
authors.lastname: Serrano
mesh_terms: D016172:DNA Fingerprinting / Q000379:methods; D017931:DNA Primers / Q000235:genetics; D015344:DNA Probes, HPV / Q000235:genetics; D005260:Female; D006801:Humans; D027383:Papillomaviridae / Q000235:genetics* / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D002578:Uterine Cervical Dysplasia / Q000175:diagnosis* / Q000235:genetics*; D014626:Vaginal Smears
chemical_list: D017931:DNA Primers; D015344:DNA Probes, HPV