title: Neuronal changes caused by Trypanosoma cruzi: an experimental model.
abstract: Define an experimental model by evaluating quantitative and morphometric changes in myenteric neurons of the colon of mice infected with Trypanosoma cruzi. Twenty-eight Swiss male mice were distributed into groups: control (CG, n=9) and inoculated with 100 (IG(100), n=9) and 1000 (IG(1000), n=10) blood trypomastigotes, Y strain-T. cruzi II. Parasitemia was evaluated from 3-25 days post inoculation (dpi) with parasites peak of 7.7 × 10(6) and 8.4 × 10(6) trypomastigotes/mL at 8(th) dpi (p>0.05) in IG(100) and IG(1000), respectively. Chronic phase of the infection was obtained with two doses of 100mg/Kg/weight and one dose of 250mg/Kg/weight of Benznidazole on 11, 16 and 18 dpi. Three animals from each group were euthanized at 18, 30 and 75 dpi. The colon was stained with Giemsa. The quantitative and morphometric analysis of neurons revealed that the infection caused a decrease of neuronal density on 30(th) dpi (p<0.05) and 75 dpi (p<0.05) in IG(100) and IG(1000). Infection caused death and neuronal hypertrophy in the 75(th) dpi in IG(100) and IG(1000) (p<0.05, p<0.01). The changes observed in myenteric neurons were directly related to the inoculate and the time of infection.
authors.affiliation: Programa de Pós-graduação em Ciências da Saúde, Universidade Estadual de Maringá, Maringá, PR, Brasil. neidemartinsenf@yahoo.com.br
authors.forename: Neide M
authors.initials: NM
authors.lastname: Moreira
authors.forename: Débora M G
authors.initials: DM
authors.lastname: Sant'ana
authors.forename: Eduardo J A
authors.initials: EJ
authors.lastname: Araújo
authors.forename: Max J O
authors.initials: MJ
authors.lastname: Toledo
authors.forename: Mônica L
authors.initials: ML
authors.lastname: Gomes
authors.forename: Silvana M de
authors.initials: SM
authors.lastname: Araújo
mesh_terms: D000818:Animals; D014355:Chagas Disease / Q000473:pathology*; D002908:Chronic Disease; D003106:Colon / Q000294:innervation* / Q000473:pathology; D004195:Disease Models, Animal; D008297:Male; D051379:Mice; D009197:Myenteric Plexus / Q000469:parasitology* / Q000473:pathology; D009474:Neurons / Q000469:parasitology* / Q000473:pathology; D018512:Parasitemia; D013997:Time Factors; D014349:Trypanosoma cruzi