title: Xenotropic murine leukaemia virus-related virus is not found in peripheral blood cells from treatment-naive human immunodeficiency virus-positive patients.
abstract: The human pathogen xenotropic murine leukaemia virus-related virus (XMRV) has been tentatively associated with prostate cancer and chronic fatigue syndrome. Unfortunately, subsequent studies failed to identify the virus in various clinical settings. To determine whether XMRV circulates in humans and the relationship with its host, we searched for the virus in 124 human immunodeficiency virus-infected patients who might have been exposed to XMRV, might be prone to infection as a result of progressive immunodeficiency, and had not yet been treated with antiretroviral drugs. Using nested PCR and single-step TaqMan real-time PCR, both designed on the XMRV gag gene, we could not find any positive samples. These findings add to the growing amount of scepticism regarding XMRV.
authors.affiliation: Virology Unit, Pisa University Hospital, Pisa, Italy. fabrizio.maggi63@gmail.com
authors.forename: F
authors.initials: F
authors.lastname: Maggi
authors.forename: D
authors.initials: D
authors.lastname: Focosi
authors.forename: L
authors.initials: L
authors.lastname: Lanini
authors.forename: S
authors.initials: S
authors.lastname: Sbranti
authors.forename: P
authors.initials: P
authors.lastname: Mazzetti
authors.forename: L
authors.initials: L
authors.lastname: Macera
authors.forename: S
authors.initials: S
authors.lastname: Davini
authors.forename: M
authors.initials: M
authors.lastname: De Donno
authors.forename: M L
authors.initials: ML
authors.lastname: Mariotti
authors.forename: G
authors.initials: G
authors.lastname: Antonelli
authors.forename: F
authors.initials: F
authors.lastname: Scatena
authors.forename: M
authors.initials: M
authors.lastname: Pistello
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001773:Blood Cells / Q000821:virology*; D015673:Fatigue Syndrome, Chronic / Q000821:virology*; D005260:Female; D015658:HIV Infections / Q000150:complications*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011471:Prostatic Neoplasms / Q000821:virology*; D060888:Real-Time Polymerase Chain Reaction; D058008:Xenotropic murine leukemia virus-related virus / Q000302:isolation & purification*; D055815:Young Adult