title: Biotypes and virulence factors of Gardnerella vaginalis isolated from cases of bacterial vaginosis.
abstract: The present study was conducted to correlate the biotypes of Gardnerella vaginalis strains isolated from cases of bacterial vaginosis and their virulence factors. Thirty-two strains of G. vaginalis isolated from cases of bacterial vaginosis were biotyped. Adherence to vaginal epithelial cells, biofilm production, surface hydrophobicity, phospholipase C and protease activity were tested on these isolates. Biotype 1 was the most prevalent (8; 25%), followed by biotype 2 (7; 21.9%) and biotypes 5 and 8 (5; 15.6%). We did not find any statistical correlation between G. vaginalis biotypes and its virulence factors. Virulence factors expressed by G. vaginalis were not associated with a single biotype.
authors.affiliation: Department of Microbiology, Kasturba Medical College, Manipal University, Mangalore 575 001, India.
authors.forename: J
authors.initials: J
authors.lastname: Udayalaxmi
authors.forename: G K
authors.initials: GK
authors.lastname: Bhat
authors.forename: S
authors.initials: S
authors.lastname: Kotigadde
mesh_terms: D000328:Adult; D001422:Bacterial Adhesion; D015373:Bacterial Typing Techniques; D018441:Biofilms / Q000254:growth & development; D004847:Epithelial Cells / Q000382:microbiology; D005260:Female; D006194:Gardnerella vaginalis / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification* / Q000472:pathogenicity; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D010447:Peptide Hydrolases / Q000378:metabolism; D010738:Type C Phospholipases / Q000378:metabolism; D016585:Vaginosis, Bacterial / Q000382:microbiology*; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D037521:Virulence Factors; D010738:Type C Phospholipases; D010447:Peptide Hydrolases