title: Survey of bromodeoxyuridine uptake among environmental bacteria and variation in uptake rates in a taxonomically diverse set of bacterial isolates.
abstract: Incorporation of 5-Bromo-2'-Deoxyuridine (BrdU) into DNA can be used to target replicating bacteria in the environment, but differential uptake capacity is a potential bias. Among 23 bacterial isolates commonly found in soils, most took up BrdU, but at up to 10-fold different cell-specific rates. Combined with results from an in silico analysis of 1000 BrdU-labeled 16S rRNA gene sequences, our results demonstrate a BrdU uptake bias with no apparent relationship between taxa affiliation and ability to incorporate BrdU.
authors.affiliation: Swedish University of Agricultural Sciences, Department of Microbiology, Box 7025, SE-750 07 Uppsala, Sweden.
authors.forename: Maria
authors.initials: M
authors.lastname: Hellman
authors.forename: Jeanette
authors.initials: J
authors.lastname: Berg
authors.forename: Kristian K
authors.initials: KK
authors.lastname: Brandt
authors.forename: Sara
authors.initials: S
authors.lastname: Hallin
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D001692:Biological Transport; D001973:Bromodeoxyuridine / Q000378:metabolism*; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology
chemical_list: D012336:RNA, Ribosomal, 16S; D004247:DNA; D001973:Bromodeoxyuridine