title: Effector-triggered immunity blocks pathogen degradation of an immunity-associated vesicle traffic regulator in Arabidopsis.
abstract: Innate immunity in plants can be triggered by microbe- and pathogen-associated molecular patterns. The pathogen-associated molecular pattern-triggered immunity (PTI) is often suppressed by pathogen effectors delivered into the host cell. Plants can overcome pathogen suppression of PTI and reestablish pathogen resistance through effector-triggered immunity (ETI). An unanswered question is how plants might overcome pathogen-suppression of PTI during ETI. Findings described in this paper suggest a possible mechanism. During Pseudomonas syringae pathovar tomato (Pst) DC3000 infection of Arabidopsis, a host ADP ribosylation factor guanine nucleotide exchange factor, AtMIN7, is destabilized by the pathogen effector HopM1 through the host 26S proteasome. In this study, we discovered that AtMIN7 is required for not only PTI, consistent with the notion that Pst DC3000 degrades AtMIN7 to suppress PTI, but also ETI. The AtMIN7 level in healthy plants is low, but increases posttranscriptionally in response to activation of PTI. Whereas DC3000 infection led to degradation of AtMIN7, activation of ETI by three different effectors, AvrRpt2, AvrPphB, and HopA1, in Col-0 plants blocks the ability of Pst DC3000 to destabilize AtMIN7. Further analyses of bacterial translocation of HopM1 and AtMIN7 stability in HopM1 transgenic plants show that ETI prevents HopM1-mediated degradation of AtMIN7 inside the plant cell. Both AtMIN7 and HopM1 are localized to the trans-Golgi network/early endosome, a subcellular compartment that is not previously known to be associated with bacterial pathogenesis in plants. Thus, blocking pathogen degradation of trans-Golgi network/early endosome-associated AtMIN7 is a critical part of the ETI mechanism to counter bacterial suppression of PTI.
authors.affiliation: Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, MI 48824, USA.
authors.forename: Kinya
authors.initials: K
authors.lastname: Nomura
authors.forename: Christy
authors.initials: C
authors.lastname: Mecey
authors.forename: Young-Nam
authors.initials: YN
authors.lastname: Lee
authors.forename: Lori Alice
authors.initials: LA
authors.lastname: Imboden
authors.forename: Jeff H
authors.initials: JH
authors.lastname: Chang
authors.forename: Sheng Yang
authors.initials: SY
authors.lastname: He
mesh_terms: D017360:Arabidopsis / Q000276:immunology* / Q000382:microbiology; D029681:Arabidopsis Proteins / Q000502:physiology; D020662:Guanine Nucleotide Exchange Factors; D054884:Host-Pathogen Interactions; D006868:Hydrolysis; D044224:Pseudomonas syringae / Q000472:pathogenicity
chemical_list: D029681:Arabidopsis Proteins; C512008:At3g43300 protein, Arabidopsis; D020662:Guanine Nucleotide Exchange Factors