title: Efficient inhibition of germination of coat-deficient bacterial spores by multivalent metal cations, including terbium (Tb³+).
abstract: Release of dipicolinic acid (DPA) and its fluorescence with terbium (Tb(3+)) allow rapid measurement of the germination and viability of spores of Bacillus and Clostridium species. However, germination of coat-deficient Bacillus spores was strongly inhibited by Tb(3+) and some other multivalent cations. Tb(3+) also inhibited germination of coat-deficient Clostridium perfringens spores.
authors.affiliation: Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030-3305, USA.
authors.forename: Xuan
authors.initials: X
authors.lastname: Yi
authors.forename: Colton
authors.initials: C
authors.lastname: Bond
authors.forename: Mahfuzur R
authors.initials: MR
authors.lastname: Sarker
authors.forename: Peter
authors.initials: P
authors.lastname: Setlow
mesh_terms: D001407:Bacillus / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism; D002412:Cations; D003013:Clostridium / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D005453:Fluorescence; D050296:Microbial Viability; D010848:Picolinic Acids; D013171:Spores, Bacterial / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D013725:Terbium / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D001426:Bacterial Proteins; D002412:Cations; D010848:Picolinic Acids; D013725:Terbium; C004860:dipicolinic acid