title: Lipoxin A(4) and 8-isoprostane in the exhaled breath condensate of children hospitalized for status asthmaticus.
abstract: OBJECTIVE
  To measure levels of 8-isoprostane and Lipoxin A4 in the exhaled breath condensate of children (7-17 yrs old) recovering from status asthmaticus in a pediatric intensive care unit and to compare their respective levels in the exhaled breath condensate collected from age-matched "healthy" children enrolled from an ambulatory pediatric clinic during well-child visits.
  
  
  METHODS
  Prospective case-controlled study.
  
  
  METHODS
  Teaching hospitals and a research laboratory.
  
  
  METHODS
  Children recovering from status asthmaticus and age-matched controls.
  
  
  METHODS
  Collection of exhaled breath condensate from patients recovering from status asthmaticus and controls for purpose of measurement of 8-isoprostane and Lipoxin A4.
  
  
  RESULTS
  There was no difference in age (11.9 ± 3.0 vs. 12.0 ± 3.3 yrs, p = .9) between patients and control subjects. All participants completed the exhaled breath condensate collection without complications. There was no difference in the pulmonary index (3.3 ± 2.2 vs. 3.1 ± 1.9, p = 1.0) after collection of exhaled breath condensate compared with baseline values in patients with status asthmaticus. The level of 8-isoprostane was significantly higher (63 ± 9 vs. 41 ± 13 pg/mL, p < .001), whereas the level of Lipoxin A4 was significantly lower (5.6 ± 2.9 vs. 10.5 ± 3.1 ng/mL, p < .001) in the exhaled breath condensate from children recovering from status asthmaticus compared with control subjects.
  
  
  CONCLUSIONS
  8-Isoprostane was elevated and Lipoxin A4 is decreased in the exhaled breath condensate of children recovering from status asthmaticus in a pediatric intensive care unit. These data may provide new insight into the pathophysiology of asthma in children in this clinical setting.
authors.affiliation: Providence Hospital and Medical Center, Toledo, OH, USA. rashedh48@gmail.com
authors.forename: Rashed A
authors.initials: RA
authors.lastname: Hasan
authors.forename: Edmund
authors.initials: E
authors.lastname: OʼBrien
authors.forename: Peter
authors.initials: P
authors.lastname: Mancuso
mesh_terms: D000293:Adolescent; D015415:Biomarkers / Q000378:metabolism; D001944:Breath Tests / Q000379:methods; D016022:Case-Control Studies; D002648:Child; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000378:metabolism; D045853:Exhalation; D005260:Female; D006760:Hospitalization / Q000706:statistics & numerical data; D006801:Humans; D044045:Lipoxins / Q000378:metabolism*; D008297:Male; D010865:Pilot Projects; D011446:Prospective Studies; D013224:Status Asthmaticus / Q000378:metabolism* / Q000628:therapy; D013997:Time Factors
chemical_list: D015415:Biomarkers; D044045:Lipoxins; C040527:lipoxin A4; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost