title: Hypoxia-induced cisplatin resistance is reversible and growth rate independent in lung cancer cells.
abstract: Hypoxia frequently occurs in solid tumors and is known to contribute to chemotherapy resistance. However, the mechanisms leading to chemotherapy resistance are not entirely known. We investigated hypoxia-induced resistance to cisplatin in NSCLC cell lines. We show that chronic moderate hypoxia induced resistance to cisplatin in NSCLC cells without involvement of selection pressure. Our data suggest that stabilization of the hypoxia-inducible factor 1 alpha and down-regulation of the pro-apoptotic protein BAX play a role in this process. Furthermore, we provide evidence that hypoxia-induced resistance to cisplatin is not due to the reduced growth rate of cancer cells under hypoxic conditions.
authors.affiliation: Division of Pulmonology, Department of Internal Medicine, Medical University of Graz, Austria.
authors.forename: Christoph
authors.initials: C
authors.lastname: Wohlkoenig
authors.forename: Katharina
authors.initials: K
authors.lastname: Leithner
authors.forename: Alexander
authors.initials: A
authors.lastname: Deutsch
authors.forename: Andelko
authors.initials: A
authors.lastname: Hrzenjak
authors.forename: Andrea
authors.initials: A
authors.lastname: Olschewski
authors.forename: Horst
authors.initials: H
authors.lastname: Olschewski
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis; D015153:Blotting, Western; D015687:Cell Hypoxia; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival; D002945:Cisplatin / Q000494:pharmacology*; D019008:Drug Resistance, Neoplasm; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000970:Antineoplastic Agents; D002945:Cisplatin