title: The characterisation of piRNA-related 19mers in the mouse.
abstract: BACKGROUND
  Piwi interacting RNA, or piRNA, is a class of small RNA almost exclusively expressed in the germline where they serve essential roles in retrotransposon silencing. There are two types, primary and secondary piRNA, and the latter is a product of enzymatic cleavage of retrotransposons' transcripts directed by the former. Recently, a new class of 19nt long RNA was discovered that is specific to testis and appears to be linked to secondary piRNA biogenesis.
  
  
  RESULTS
  We locate clusters of the testis-specific 19mers, which we call piRNA-related 19mers (pr19RNA), and characterise the transcripts from which they are derived. Most pr19RNA clusters were associated with retrotransposons and unannotated antisense transcripts overlapping piRNA clusters. At these loci the abundance of 19mers was found to be greater than that of secondary piRNAs.
  
  
  CONCLUSIONS
  We find that pr19RNAs are distinguished from other RNA populations by their length and flanking sequence, allowing their identification without requiring overlapping piRNAs. Using such sequence features allows identification of the source transcripts, and we suggest that these likely represent the substrates of primary piRNA-guided RNA cleavage events. While pr19RNAs appear not to bind directly to Miwi or Mili, their abundance relative to secondary piRNAs, in combination with their precise length, suggests they may be more than by-products of secondary piRNA biogenesis.
authors.affiliation: Queensland Institute of Medical Research, Epigenetics, Herston, Brisbane 4006, Australia.
authors.forename: Harald M
authors.initials: HM
authors.lastname: Oey
authors.forename: Neil A
authors.initials: NA
authors.lastname: Youngson
authors.forename: Emma
authors.initials: E
authors.lastname: Whitelaw
mesh_terms: D000818:Animals; D001483:Base Sequence; D016000:Cluster Analysis; D056426:Genetic Loci; D008297:Male; D051379:Mice; D034741:RNA, Small Interfering / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D018626:Retroelements / Q000235:genetics; D013091:Spermatogenesis; D013737:Testis / Q000378:metabolism
chemical_list: D034741:RNA, Small Interfering; D018626:Retroelements