title: [Analysis of genetic relationships of Pueraria thomsonii based on SRAP markers].
abstract: OBJECTIVE
  To determine the interspecies relationships of 18 Pueraria thomsonii cultivars in molecular level.
  
  
  METHODS
  Eighteen P. thomsonii cultivars were evaluated by using sequence-related amplified polymorphism (SRAP) markers, with P. lobata and P. peduncularis as contrast species. Systematic relationships were constructed based on the UPGMA method by TREECONW software.
  
  
  RESULTS
  The results showed that 22 primer pairs produced 338 loci, out of which 216 were polymorphic, the percentage of polymorphic loci was 63.9%. An average of 15.4 loci and 9.8 polymorphic loci were generated by each pair of primers. Genetic distance was analyzed by TREECONW software. Genetic distance of 18 P. thomsonii were changed from 0.004 7 to 0.265 8, with an average of 0.316. Using cluster analysis (UPGMA) based on those polymorphism bands amplified with SRAP primers, the 22 cultivars were classified into three groups, groups 1 with 18 P. thomsonii, group 2 with 3 P. lobata, and group 3 with 1 P. peduncularis. Most of the P. thomsonii from the same region were not in the same group.
  
  
  CONCLUSIONS
  SRAP markers could be a good marker for genetic relationship research in the P. thomsonii.
authors.affiliation: Institute of Material Medical Planting, Chongqing Academy of Chinese Materia Medica, Chongqing Engineering Research Center For Fine Variety Breeding Techniques of Chinese Materia Medica, Chongqing 400065, China. 17837@163.com
authors.forename: Daxia
authors.initials: D
authors.lastname: Chen
authors.forename: Rui
authors.initials: R
authors.lastname: Peng
authors.forename: Longyun
authors.initials: L
authors.lastname: Li
authors.forename: Xue
authors.initials: X
authors.lastname: Zhang
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
mesh_terms: D005819:Genetic Markers; D010802:Phylogeny; D011110:Polymorphism, Genetic; D029908:Pueraria / Q000145:classification* / Q000235:genetics*
chemical_list: D005819:Genetic Markers