title: Accuracy of MIC determination for Streptococcus pneumoniae using the Sirscan2000automatic MIC determination system.
abstract: The Sirscan2000automatic MIC determination (SIR-MD) system is a new system for MIC determination based on the automatic detection of growth of bacteria spotted onto agar medium using a camera scan. To evaluate its accuracy, 3608 Streptococcus pneumoniae clinical isolates yielding 18,165 MICs were tested in parallel with the SIR-MD and a standard interpretive antibiogram procedure. The overall percent agreement between the 2 methods within 1 log(2) dilution was 86.9%. After exclusion of the 11.8% noninterpretable results, errors in the deduction of susceptibilities were very major in 0.03%, major in 0.2%, and minor in 1.3%.
authors.affiliation: Service de Microbiologie, AP-HP, Hôpital Européen Georges Pompidou, Paris F-75015, France. patrick.grohs@egp.aphp.fr
authors.forename: Patrick
authors.initials: P
authors.lastname: Grohs
authors.forename: Claire
authors.initials: C
authors.lastname: Janoir
authors.forename: Sophie
authors.initials: S
authors.lastname: Grondin
authors.forename: Sylvie
authors.initials: S
authors.lastname: Simon
authors.forename: Gaëlle
authors.initials: G
authors.lastname: Bonnet
authors.forename: Laura
authors.initials: L
authors.lastname: Henry
authors.forename: Laurent
authors.initials: L
authors.lastname: Gutmann
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Varon
mesh_terms: D000362:Agar; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001331:Automation / Q000379:methods*; D003470:Culture Media / Q000737:chemistry; D003951:Diagnostic Errors / Q000706:statistics & numerical data*; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods*; D013296:Streptococcus pneumoniae / Q000187:drug effects* / Q000254:growth & development
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D000362:Agar