title: A sensitive flow injection chemiluminescence method for the determination of progesterone.
abstract: A sensitive flow injection (FI) chemiluminescence (CL) method was developed for the determination of trace amounts of progesterone. This method was based on the luminescent properties of the tris(1,10-phenanthroline) ruthenium(II) - potassium permanganate (KMnO4 ) - progesterone in acidic medium sensitized by Na2 SO3 . With the peak height as a quantitative parameter applying optimum conditions, the relative CL intensity was linear with progesterone concentration in the range of 1.0 × 10(-10) ∼ 6.0 × 10(-9) g·ml(-1) and 6.0 × 10(-9) ∼ 4.0 × 10(-8) g·ml(-1) with a detection limit of 7.1 × 10(-11) g·ml(-1) . The relative standard deviation (RSD) was 2.79% for 1.0 × 10(-8) g·ml(-1) progesterone (n = 11). The proposed method held low detection limit and was successfully applied to determination of progesterone in pharmaceutical preparations. The possible CL reaction mechanism was also discussed.
authors.affiliation: School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China. jncw88@163.com
authors.forename: Wei
authors.initials: W
authors.lastname: Cao
authors.forename: Pixue
authors.initials: P
authors.lastname: Gong
authors.forename: Wenwen
authors.initials: W
authors.lastname: Liu
authors.forename: Mei
authors.initials: M
authors.lastname: Zhuang
authors.forename: Jinghe
authors.initials: J
authors.lastname: Yang
mesh_terms: D004867:Equipment Design; D017022:Flow Injection Analysis / Q000295:instrumentation*; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000295:instrumentation*; D009942:Organometallic Compounds / Q000737:chemistry*; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D010618:Phenanthrolines / Q000737:chemistry*; D011196:Potassium Permanganate / Q000737:chemistry*; D011374:Progesterone / Q000032:analysis*
chemical_list: D009942:Organometallic Compounds; D004364:Pharmaceutical Preparations; D010618:Phenanthrolines; D011196:Potassium Permanganate; C034282:tris-(1,10-phenanthroline)ruthenium; D011374:Progesterone