title: Evaluation of p53 genotype on gene expression in the testis, liver, and heart from male C57BL/6 mice.
abstract: Our laboratory is conducting experiments designed to characterize the role of p53 in gene expression in the TSG-p53® mouse model. In the study reported here, gene expression levels in tissue derived from the testis, liver, and heart of male, 8-9 week old, p53 wild-type (WT), heterozygous (HET) or knockout (KO) mice were determined utilizing a targeted qPCR 84-gene array. The heart, liver and testis were selected because of the unique function and rate of cell division of each tissue. The genes on the arrays were categorized into three Functional Gene Groups, Apoptosis, Cell-Cycle and DNA Repair. Differences in expression of the functional groups were determined by multivariate analysis of variance (MANOVA) and significant (P < 0.05) differences in their expression were found among the heart, liver and testis. Further, the expression of the Functional Gene Groups in each of these tissues was also significantly affected by p53 genotype. These data indicate that gene expression in unperturbed tissue is influenced by the status of p53 genotype, and relates, at least partially, to the function of the tissue.
authors.affiliation: Division of Genetic and Molecular Toxicology, National Center for Toxicological Research, FDA, Jefferson, AR, USA. dayton.petibone@fda.hhs.gov
authors.forename: Dayton M
authors.initials: DM
authors.lastname: Petibone
authors.forename: Rohan M
authors.initials: RM
authors.lastname: Kulkarni
authors.forename: Ching-Wei
authors.initials: CW
authors.lastname: Chang
authors.forename: James J
authors.initials: JJ
authors.lastname: Chen
authors.forename: Suzanne M
authors.initials: SM
authors.lastname: Morris
mesh_terms: D000818:Animals; D017209:Apoptosis; D059447:Cell Cycle Checkpoints; D002455:Cell Division; D004260:DNA Repair; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D016158:Genes, p53; D006579:Heterozygote; D008099:Liver / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D015999:Multivariate Analysis; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D018384:Oxidative Stress; D016133:Polymerase Chain Reaction / Q000379:methods; D012313:RNA / Q000235:genetics / Q000378:metabolism; D013737:Testis / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism*
chemical_list: D016159:Tumor Suppressor Protein p53; D012313:RNA