title: Anticoagulant, antiherpetic and antibacterial activities of sulphated polysaccharide from Indian medicinal plant Tridax procumbens L. (Asteraceae).
abstract: The sulphated polysaccharide from the widespread Tridax procumbens plant was studied for the anticoagulant, antiherpetic and antibacterial activity. The anticoagulant activity was determined by the activated partial thromboplastin time assay. The sulphated polysaccharide from T. procumbens represented potent anticoagulant reaching the efficacy to heparin and chondroitin sulphate. Moreover, the sulphated polysaccharide extracted from T. procumbens was found non-toxic on Vero cell lines up to the concentration of 200 μg/ml. Sulphated polysaccharide exhibited detectable antiviral effect towards HSV-1 with IC(50) value 100-150 μg/ml. Furthermore, sulphated polysaccharide from T. procumbens was highly inhibitory against the bacterial strains Vibrio alginolyticus and Vibrio harveyi isolated from oil sardine.
authors.affiliation: Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, 603203 Tamil Nadu, India.
authors.forename: Shabeena Yousuf
authors.initials: SY
authors.lastname: Naqash
authors.forename: R A
authors.initials: RA
authors.lastname: Nazeer
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D000925:Anticoagulants / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D000998:Antiviral Agents / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D019659:Asteraceae / Q000737:chemistry*; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D002522:Chlorocebus aethiops; D002809:Chondroitin Sulfates / Q000494:pharmacology; D006470:Hemorrhage / Q000188:drug therapy*; D006493:Heparin / Q000494:pharmacology; D006561:Herpes Simplex / Q000188:drug therapy* / Q000821:virology; D018259:Herpesvirus 1, Human / Q000187:drug effects; D020128:Inhibitory Concentration 50; D010314:Partial Thromboplastin Time; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D010946:Plants, Medicinal / Q000737:chemistry; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D014709:Vero Cells; D014735:Vibrio Infections / Q000188:drug therapy* / Q000382:microbiology; D044149:Vibrio alginolyticus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D000925:Anticoagulants; D000998:Antiviral Agents; D010936:Plant Extracts; D011134:Polysaccharides; D006493:Heparin; D002809:Chondroitin Sulfates