title: Assays for the antiangiogenic and neurotrophic serpin pigment epithelium-derived factor.
abstract: Pigment epithelium-derived factor (PEDF) is a secreted serpin that exhibits a variety of interesting biological activities. The multifunctional PEDF has neurotrophic and antiangiogenic properties, and acts in retinal differentiation, survival, and maintenance. It is also antitumorigenic and antimetastatic, and has stem cell self-renewal properties. It is widely distributed in the human body and exists in abundance in the eye as a soluble extracellular glycoprotein. Its levels are altered in diseases characterized by retinopathies and angiogenesis. Its mechanisms of neuroprotection and angiogenesis are associated with receptor interactions at cell-surface interfaces and changes in protein expression. This serpin lacks demonstrable serine protease inhibitory activity, but has binding affinity to extracellular matrix components and cell-surface receptors. Here we describe purification protocols, methods to quantify PEDF, and determine interactions with specific molecules, as well as neurotrophic and angiogenesis assays for this multifunctional protein.
authors.affiliation: Section of Protein Structure and Function, National Eye Institute, NIH, Bethesda, Maryland, USA.
authors.forename: Preeti
authors.initials: P
authors.lastname: Subramanian
authors.forename: Susan E
authors.initials: SE
authors.lastname: Crawford
authors.forename: S Patricia
authors.initials: SP
authors.lastname: Becerra
mesh_terms: D000645:Ammonium Sulfate / Q000737:chemistry; D000818:Animals; D001082:Aqueous Humor / Q000737:chemistry; D002417:Cattle; D002852:Chromatography, Ion Exchange; D004797:Enzyme-Linked Immunosorbent Assay; D005136:Eye Proteins / Q000032:analysis* / Q000302:isolation & purification; D007120:Immunochemistry; D009414:Nerve Growth Factors / Q000032:analysis* / Q000302:isolation & purification; D015843:Serpins / Q000032:analysis* / Q000302:isolation & purification; D014822:Vitreous Body / Q000737:chemistry; D000099174:Pigment Epithelium-Derived Factor
chemical_list: D000645:Ammonium Sulfate; D005136:Eye Proteins; D009414:Nerve Growth Factors; D015843:Serpins; D000099174:Pigment Epithelium-Derived Factor