title: The diagnostic value of circulating stanniocalcin-1 mRNA in non-small cell lung cancer.
abstract: OBJECTIVE
  Previous studies have suggested that the aberrant expression of Stanniocalcin-1 (STC1) occurs in tumor cells. In this study, we explored whether the circulating STC1 mRNA is a promising biomarker in the peripheral blood in patients with non-small cell lung cancer (NSCLC).
  
  
  METHODS
  The level of circulating STC1 mRNA was determined with a sensitive quantitative real-time reverse transcription PCR assay. and the data were analyzed by the statistical methods of one-way ANOVA, Mann-Whitney-Wilcoxon U-Test, and Receiver operating characteristic (ROC) curve analysis.
  
  
  RESULTS
  The level of circulating STC1 mRNA in patients with NSCLC was significantly higher than in patients with benign pulmonary disease (P < 0.001) or healthy volunteers (P < 0.001). Higher levels of circulating STC1 mRNA were associated with more advanced tumor stages and histological subtypes. Using a cutoff of 0.055, the sensitivity and specificity of STC1 mRNA levels to differentiate between patients with NSCLC and patients with benign pulmonary diseases was 66.7 and 90.9%, and it was 63.7 and 99.8% for patients with NSCLC and healthy volunteers, respectively.
  
  
  CONCLUSIONS
  These findings support our hypothesis that circulating STC1 mRNA is a promising biomarker in the peripheral blood.
authors.affiliation: Department of Medical Laboratory, Shanghai Sixth Hospital, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Yu-Zhen
authors.initials: YZ
authors.lastname: Du
authors.forename: Xiao-Hua
authors.initials: XH
authors.lastname: Gu
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood* / Q000235:genetics; D002289:Carcinoma, Non-Small-Cell Lung / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D016022:Case-Control Studies; D003937:Diagnosis, Differential; D005260:Female; D006023:Glycoproteins / Q000097:blood* / Q000235:genetics; D006801:Humans; D008171:Lung Diseases / Q000175:diagnosis / Q000235:genetics / Q000473:pathology; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009360:Neoplastic Cells, Circulating / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis* / Q000097:blood; D012372:ROC Curve; D060888:Real-Time Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D014408:Biomarkers, Tumor; D006023:Glycoproteins; D012333:RNA, Messenger; C051795:teleocalcin