title: Molecular cloning and expression analysis of two distinct F-type lectins from the rock bream, Oplegnathus fasciatus.
abstract: Several lectin families characterized by distinct signature sequence motifs and structural folds, such as C-type, peptidoglycan recognition protein, ficolin, pentraxins, and most recently galectins, have been implicated in immune surveillance. In this study, two distinct F-type lectins RbFTL-1 and RbFTL-2, from the rock bream (Oplegnathus fasciatus), were identified and their expression was analyzed. The full-length cDNA of RbFTL-1 was composed of 1204 bp with a 945-bp open reading frame (ORF) that encoded a 314 amino-acid protein, while that of RbFTL-2 consisted of 1614 bp with a 951-bp ORF encoding a 316 amino-acid protein. RbFTL-1 and RbFTL-2 mRNAs were predominately expressed in the head-kidney and in the liver, respectively. Levels of the RbFTL-1 mRNA transcript increased up to 5.0- and 2.8-fold in the head-kidney and trunk-kidney compared to the muscle, respectively, while those of the RbFTL-2 mRNA transcript increased up to 12.0-fold in liver. The expression of RbFTL-1 and RbFTL-2 were differentially up-regulated in rock bream challenged with Edwardsiella tarda, Streptococcus iniae, and RSIV, with significant increases at 1 and 3h post-challenge compared to the controls.
authors.affiliation: Department of Marine Biology & Aquaculture, Institute of Marine Industry, College of Marine Science, Gyeongsang National University, 455, Tongyeong 650-160, Republic of Korea.
authors.forename: Hyung-Jun
authors.initials: HJ
authors.lastname: Park
authors.forename: Ju-Won
authors.initials: JW
authors.lastname: Kim
authors.forename: Eun-Gyeong
authors.initials: EG
authors.lastname: Kim
authors.forename: Ha-Na
authors.initials: HN
authors.lastname: Kim
authors.forename: Young-Sun
authors.initials: YS
authors.lastname: Chae
authors.forename: Ji-Min
authors.initials: JM
authors.lastname: Jeong
authors.forename: Do-Hyung
authors.initials: DH
authors.lastname: Kim
authors.forename: Chan-Il
authors.initials: CI
authors.lastname: Park
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D004266:DNA Virus Infections / Q000276:immunology*; D020609:Edwardsiella tarda / Q000276:immunology* / Q000472:pathogenicity; D004756:Enterobacteriaceae Infections / Q000276:immunology*; D005399:Fishes / Q000276:immunology / Q000821:virology; D060226:Head Kidney / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology / Q000821:virology; D007113:Immunity, Innate / Q000235:genetics; D017994:Iridovirus / Q000276:immunology* / Q000472:pathogenicity; D037102:Lectins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D008099:Liver / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology / Q000821:virology; D008969:Molecular Sequence Data; D009928:Organ Specificity; D013290:Streptococcal Infections / Q000276:immunology*; D013291:Streptococcus / Q000276:immunology* / Q000472:pathogenicity; D015854:Up-Regulation / Q000276:immunology
chemical_list: D037102:Lectins