title: Using laser ablation/inductively coupled plasma mass spectrometry to bioimage multiple elements in mouse tumors after hyperthermia.
abstract: In this study, we employed laser ablation/inductively coupled plasma mass spectrometry (LA-ICP-MS) to map the spatial distribution of Gd-doped iron oxide nanoparticles (IONPs) in one tumor slice that had been subjected to magnetic fluid hyperthermia (MFH). The mapping results revealed the high resolution of the elemental analysis, with the distribution of Gd atoms highly correlated with that of the Fe atoms. The spatial distributions of C, P, S, and Zn atoms revealed that the effect of MFH treatment was significantly dependent on the diffusion of the magnetic fluid in the tissue. An observed enrichment of Cu atoms after MFH treatment was probably due to inflammation in the tumor. The abnormal distribution of Ni atoms suggests a probable biochemical reaction in the tumor. Therefore, this LA-ICP-MS mapping technique can provide novel information regarding the spatial distribution of elements in tumors after cancer therapy.
authors.affiliation: Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu 30013, Taiwan.
authors.forename: Yi-Kong
authors.initials: YK
authors.lastname: Hsieh
authors.forename: Pei-Shin
authors.initials: PS
authors.lastname: Jiang
authors.forename: Bing-Shen
authors.initials: BS
authors.lastname: Yang
authors.forename: Tian-Ye
authors.initials: TY
authors.lastname: Sun
authors.forename: Hsu-Hsia
authors.initials: HH
authors.lastname: Peng
authors.forename: Chu-Fang
authors.initials: CF
authors.lastname: Wang
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004602:Elements; D005290:Ferric Compounds / Q000737:chemistry; D005682:Gadolinium / Q000737:chemistry; D006358:Hot Temperature; D006801:Humans; D053685:Laser Therapy; D008297:Male; D013058:Mass Spectrometry; D053768:Metal Nanoparticles / Q000737:chemistry; D019216:Metals, Heavy / Q000737:chemistry*; D051379:Mice; D008810:Mice, Inbred C57BL; D009369:Neoplasms / Q000737:chemistry* / Q000000981:diagnostic imaging / Q000648:ultrastructure; D011471:Prostatic Neoplasms / Q000737:chemistry* / Q000000981:diagnostic imaging; D011859:Radiography; D013194:Staining and Labeling
chemical_list: D004602:Elements; D005290:Ferric Compounds; D019216:Metals, Heavy; C000499:ferric oxide; D005682:Gadolinium