title: Fructo-oligosaccharide production from inulin through partial citric or phosphoric acid hydrolyses.
abstract: Purified inulin from Dahlia tubers was partially hydrolyzed to form fructo-oligosaccharides by using citric or phosphoric acids (pH, 2.0-2.5) as mild acid catalysts. The ideal kinetic conditions to ensure a high yield of fructo-oligosaccharides relative to free fructose were a temperature range of 85°C-95°C, a hydrolysis time of 15-25 minutes, and a catalyst pH of 2.5. At the higher temperature and the longest hydrolysis time, an inversion of the product ratio occurred. Under these conditions, co-generation of hydroxymethylfurfural occurred, and it was eliminated by activated charcoal. Unlike in classic hydrolysis with hydrochloric or sulfuric acid, deionization of the actual hydrolysates was not necessary because the catalyst neutralization with common bases results in the formation of co-nutrients with alternative uses as foods or fermentation substrates. These whole hydrolysates can be advantageously added as nutraceuticals to carbonated beverages and acidic foods, such as soft drinks and yogurts.
authors.affiliation: Biomass Chemo/Biotechnology Laboratory, Department of Pharmacy, Jardim Botânico Campus, Federal University of Paraná (UFPR), Curitiba, Paraná, Brazil. jfontana@ufpr.br
authors.forename: José Domingos
authors.initials: JD
authors.lastname: Fontana
authors.forename: Adelia
authors.initials: A
authors.lastname: Grzybowski
authors.forename: Marcela
authors.initials: M
authors.lastname: Tiboni
authors.forename: Maurício
authors.initials: M
authors.lastname: Passos
mesh_terms: D002384:Catalysis; D019343:Citric Acid / Q000378:metabolism*; D031944:Dahlia / Q000737:chemistry; D005503:Food Additives; D005632:Fructose / Q000378:metabolism*; D005662:Furaldehyde / Q000031:analogs & derivatives / Q000378:metabolism; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007444:Inulin / Q000378:metabolism*; D009844:Oligosaccharides / Q000378:metabolism*; D010756:Phosphoric Acids / Q000378:metabolism*; D013997:Time Factors
chemical_list: D005503:Food Additives; D009844:Oligosaccharides; D010756:Phosphoric Acids; D019343:Citric Acid; D005632:Fructose; C008046:5-hydroxymethylfurfural; D007444:Inulin; D005662:Furaldehyde; C030242:phosphoric acid