title: Norcantharidin inhibits proliferation and fibronectin expression of HK-2 cells induced by albumin in vitro.
abstract: The present study aims to observe the effects of NCTD (norcantharidin) on proliferation and FN (fibronectin) expression in human renal proximal tubular epithelial cell line (HK-2) induced by albumin in vitro. HK-2 cells were divided into control group, albumin group and different concentration of NCTD intervention groups. Proliferation of HK-2 cells was determined by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide], FN protein in culture media of HK-2 cells was examined by ELISA, and FN mRNA was analysed by RT-PCR (reverse transcription-PCR). We chose less than 5.0 mg/l of NCTD as the experimental concentration for the cytotoxicity test. MTT score was higher in the albumin group than in the control group (P<0.05). As compared with that of the albumin group, MTT score and FN protein concentration decreased, FN mRNA significantly down-regulated in NCTD intervention groups respectively (P<0.05). Our study showed that NCTD could inhibit the albumin-induced cell proliferation and FN expression in HK-2 cells, which might further prove the anti-fibrotic role of NCTD in proteinuria-associated tubulointerstitial damage.
authors.affiliation: Division of Nephrology, The Second Xiangya Hospital, Central-South University in Hunan, Chang sha, Peoples Republic of China. liyingdoctor@yahoo.com.cn
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.forename: Fu You
authors.initials: FY
authors.lastname: Liu
authors.forename: You Ming
authors.initials: YM
authors.lastname: Peng
authors.forename: Ming
authors.initials: M
authors.lastname: Zhan
authors.forename: Shao Bin
authors.initials: SB
authors.lastname: Duan
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Lin
authors.initials: L
authors.lastname: Sun
authors.forename: Kun
authors.initials: K
authors.lastname: Ye
authors.forename: Jun Hui
authors.initials: JH
authors.lastname: Luo
mesh_terms: D000418:Albumins / Q000378:metabolism*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology*; D002460:Cell Line; D049109:Cell Proliferation; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005353:Fibronectins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000418:Albumins; D000970:Antineoplastic Agents; D019086:Bridged Bicyclo Compounds, Heterocyclic; D005353:Fibronectins; D012333:RNA, Messenger; C069741:norcantharidin