title: MicroRNA expression profiling in neurogenesis of adipose tissue-derived stem cells.
abstract: Adipose tissue-derived stem cells (ADSCs) are one population of adult stem cells that can self renew and differentiate into multiple lineages. Because of advantages in method and quantity of acquisition, ADSCs are gaining attention as an alternative source of bone marrow mesenchymal stem cells. In this study, we performed microRNA profiling of undifferentiated and of neurally-differentiated ADSCs to identify the responsible microRNAs in neurogenesis using this type of stem cell. MicroRNAs from four different donors were analysed by microarray. Compared to the undifferentiation control, we identified 39-101 microRNAs with more than two-fold higher expression and 3-9 microRNAs with two-fold lower expression. The identified microRNAs were further analysed in terms of gene ontology (GO) in relation with neurogenesis, based on their target mRNAs predicted by computational analysis. This study revealed the specific microRNAs involved in neurogenesis via microRNA microarray, and may provide the basic information for genetic induction of adult stem cell differentiation using microRNAs.
authors.affiliation: Adult Stem Cell Research Institute, Department of Obstetrics and Gynecology, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, 108 Pyung-Dong, Jongro-Gu, Seoul 110-746, Korea.
authors.forename: Jung Ah
authors.initials: JA
authors.lastname: Cho
authors.forename: Ho
authors.initials: H
authors.lastname: Park
authors.forename: Eun Hye
authors.initials: EH
authors.lastname: Lim
authors.forename: Kyo Won
authors.initials: KW
authors.lastname: Lee
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D020869:Gene Expression Profiling; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D055495:Neurogenesis / Q000235:genetics*; D013234:Stem Cells / Q000166:cytology*; D017154:Stromal Cells / Q000166:cytology
chemical_list: D035683:MicroRNAs