title: Infiltration of CD14-positive macrophages at the invasive front indicates a favorable prognosis in colorectal cancer patients with lymph node metastasis.
abstract: OBJECTIVE
  To clarify the distribution of CD14+ macrophages in colorectal cancer using flow cytometry and immunohistochemistry, and to elucidate the roles of CD14+ macrophages in colorectal cancer.
  
  
  METHODS
  We studied the paired cancerous and corresponding normal tissues from 52 patients with colorectal cancer for the distribution of CD14+, CD1a+, CD83+ and CD68+ cells, and correlated the findings with the clinicopathological characteristics and with the expression of CD86 and CD80 in the CD14+ macrophages, which are co-stimulatory factors for T cell activation.
  
  
  RESULTS
  1) CD14+ macrophages were distributed predominantly at the invasive front of colorectal cancer tissues, rather than in the normal tissues, 2) a high percentage of the CD14+ macrophages expressed CD86 and CD80, and 3) in the colorectal cancer cases with lymph node metastasis, the 5-year overall survival rate of the high CD14 group, in which the number of CD14+ macrophages was higher than the median, was better than that of the low CD14 group.
  
  
  CONCLUSIONS
  The infiltration of CD140 macrophages at the invasive front indicates a favorable prognosis in colorectal cancer patients with lymph node metastasis. In addition, the activation of CD14+ macrophages and T cells may facilitate the development of new immunotherapeutic strategies for colorectal cancer patients.
authors.affiliation: Department of Surgery, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Kinouchi
authors.forename: Koh
authors.initials: K
authors.lastname: Miura
authors.forename: Takayuki
authors.initials: T
authors.lastname: Mizoi
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Ishida
authors.forename: Wataru
authors.initials: W
authors.lastname: Fujibuchi
authors.forename: Toshinori
authors.initials: T
authors.lastname: Ando
authors.forename: Nobuki
authors.initials: N
authors.lastname: Yazaki
authors.forename: Kazuya
authors.initials: K
authors.lastname: Saito
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Shiiba
authors.forename: Iwao
authors.initials: I
authors.lastname: Sasaki
mesh_terms: D000368:Aged; D018122:B7-1 Antigen / Q000032:analysis; D051940:B7-2 Antigen / Q000032:analysis; D015179:Colorectal Neoplasms / Q000276:immunology / Q000401:mortality / Q000473:pathology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D018950:Lipopolysaccharide Receptors / Q000032:analysis*; D008207:Lymphatic Metastasis; D008264:Macrophages / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D011379:Prognosis
chemical_list: D018122:B7-1 Antigen; D051940:B7-2 Antigen; C497964:CD86 protein, human; D018950:Lipopolysaccharide Receptors