title: A comprehensive transcriptome and immune-gene repertoire of the lepidopteran model host Galleria mellonella.
abstract: BACKGROUND
  The larvae of the greater wax moth Galleria mellonella are increasingly used (i) as mini-hosts to study pathogenesis and virulence factors of prominent bacterial and fungal human pathogens, (ii) as a whole-animal high throughput infection system for testing pathogen mutant libraries, and (iii) as a reliable host model to evaluate the efficacy of antibiotics against human pathogens. In order to compensate for the lack of genomic information in Galleria, we subjected the transcriptome of different developmental stages and immune-challenged larvae to next generation sequencing.
  
  
  RESULTS
  We performed a Galleria transcriptome characterization on the Roche 454-FLX platform combined with traditional Sanger sequencing to obtain a comprehensive transcriptome. To maximize sequence diversity, we pooled RNA extracted from different developmental stages, larval tissues including hemocytes, and from immune-challenged larvae and normalized the cDNA pool. We generated a total of 789,105 pyrosequencing and 12,032 high-quality Sanger EST sequences which clustered into 18,690 contigs with an average length of 1,132 bases. Approximately 40% of the ESTs were significantly similar (E ≤ e-03) to proteins of other insects, of which 45% have a reported function. We identified a large number of genes encoding proteins with established functions in immunity related sensing of microbial signatures and signaling, as well as effector molecules such as antimicrobial peptides and inhibitors of microbial proteinases. In addition, we found genes known as mediators of melanization or contributing to stress responses. Using the transcriptomic data, we identified hemolymph peptides and proteins induced upon immune challenge by 2D-gelelectrophoresis combined with mass spectrometric analysis.
  
  
  CONCLUSIONS
  Here, we have developed extensive transcriptomic resources for Galleria. The data obtained is rich in gene transcripts related to immunity, expanding remarkably our knowledge about immune and stress-inducible genes in Galleria and providing the complete sequences of genes whose primary structure have only partially been characterized using proteomic methods. The generated data provide for the first time access to the genetic architecture of immunity in this model host, allowing us to elucidate the molecular mechanisms underlying pathogen and parasite response and detailed analyses of both its immune responses against human pathogens, and its coevolution with entomopathogens.
authors.affiliation: Institute of Phytopathology and Applied Zoology, University of Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany.
authors.forename: Heiko
authors.initials: H
authors.lastname: Vogel
authors.forename: Boran
authors.initials: B
authors.lastname: Altincicek
authors.forename: Gernot
authors.initials: G
authors.lastname: Glöckner
authors.forename: Andreas
authors.initials: A
authors.lastname: Vilcinskas
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D020869:Gene Expression Profiling; D017344:Genes, Insect / Q000235:genetics* / Q000276:immunology*; D054884:Host-Pathogen Interactions; D006801:Humans; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000494:pharmacology; D007915:Lepidoptera / Q000166:cytology / Q000235:genetics* / Q000276:immunology* / Q000502:physiology; D052981:Metarhizium / Q000187:drug effects / Q000201:enzymology; D008969:Molecular Sequence Data; D040901:Proteomics; D015398:Signal Transduction / Q000235:genetics; D013312:Stress, Physiological / Q000235:genetics
chemical_list: D019476:Insect Proteins