title: Coelomic fluid: a noninvasive source of DNA in earthworms.
abstract: To investigate whether coelomic fluid secreted by earthworms can be a noninvasive source of DNA, we amplified and sequenced DNA extracted from the coelomic fluid and muscle tissue of eight worms. The sequences obtained using DNA extracted from both sources were identical. All cytochrome c oxidase subunit I (COI) mitochondrial DNA sequences, including those retrieved from GenBank, formed a monophyletic group of Metaphire sieboldi. The results indicate that we successfully extracted total DNA from coelomic fluid secreted by earthworm.
authors.affiliation: Faculty of Agriculture, Kochi University, Monobe, Nankoku 783-8502, Japan. b0mf0252000@yahoo.co.jp
authors.forename: Yukio
authors.initials: Y
authors.lastname: Minamiya
authors.forename: Kyohei
authors.initials: K
authors.lastname: Ohga
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hayakawa
authors.forename: Katsura
authors.initials: K
authors.lastname: Ito
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Fukuda
mesh_terms: D000818:Animals; D012634:Bodily Secretions / Q000737:chemistry; D016000:Cluster Analysis; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification*; D003576:Electron Transport Complex IV / Q000235:genetics; D008969:Molecular Sequence Data; D009835:Oligochaeta / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D017422:Sequence Analysis, DNA
chemical_list: D004272:DNA, Mitochondrial; D003576:Electron Transport Complex IV