title: Cell compatible trimethoprim-decorated iron oxide nanoparticles bind dihydrofolate reductase for magnetically modulating focal adhesion of mammalian cells.
abstract: On the basis of the high affinity binding of trimethoprim (TMP) to Escherichia coli dihydrofolate reductase (eDHFR), TMP-decorated iron oxide nanoparticles bind to eDHFR with high affinity and specificity, which allows magnetic modulation of focal adhesion of mammalian cells adhered to a surface. Besides being the first example of nanoparticles that selectively bind to eDHFR, the biocompatibility of the conjugate of TMP-iron oxide nanoparticles renders a convenient and versatile platform for investigating the cellular responses to specific, mechanical perturbation of proteins via a magnetic force.
authors.affiliation: Graduate Program in Biochemistry and Biophysics, Brandeis University, 415 South Street, Waltham, Massachusetts 02454, USA.
authors.forename: Marcus J C
authors.initials: MJ
authors.lastname: Long
authors.forename: Yue
authors.initials: Y
authors.lastname: Pan
authors.forename: Hsin-Chieh
authors.initials: HC
authors.lastname: Lin
authors.forename: Lizbeth
authors.initials: L
authors.lastname: Hedstrom
authors.forename: Bing
authors.initials: B
authors.lastname: Xu
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity; D019556:COS Cells; D002470:Cell Survival / Q000187:drug effects; D002522:Chlorocebus aethiops; D004926:Escherichia coli / Q000201:enzymology; D005290:Ferric Compounds / Q000737:chemistry*; D022001:Focal Adhesions; D006367:HeLa Cells; D006801:Humans; D060328:Magnetic Phenomena; D008958:Models, Molecular; D053758:Nanoparticles / Q000737:chemistry*; D011485:Protein Binding; D011487:Protein Conformation; D013762:Tetrahydrofolate Dehydrogenase / Q000737:chemistry / Q000378:metabolism*; D014295:Trimethoprim / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D005290:Ferric Compounds; C000499:ferric oxide; D014295:Trimethoprim; D013762:Tetrahydrofolate Dehydrogenase