title: The P7-1 protein of southern rice black-streaked dwarf virus, a fijivirus, induces the formation of tubular structures in insect cells.
abstract: Southern rice black-streaked dwarf virus (SRBSDV), an insect- and plant-infecting reovirus of the genus Fijivirus, induced the formation of virus-containing tubules in infected plant and insect vector cells. Expression of the nonstructural protein P7-1 of SRBSDV in insect cells by a recombinant baculovirus resulted in the formation of tubules with dimensions and appearance similar to those found in SRBSDV-infected cells. These tubules protruded from the cell surface, supporting the hypothesis that the P7-1 protein contains two putative transmembrane domains that are necessary for the formation of tubules. Furthermore, the self-interaction of SRBSDV P7-1 protein indicates that this protein has the capacity to form homodimers or oligomers to assemble the proposed helical symmetry structure of tubules. Taken together, our results indicate that SRBSDV P7-1 has the intrinsic ability of self-interaction to form tubules growing from the cell surface in the absence of other viral proteins.
authors.affiliation: Institute of Plant Virology, Fujian Province Key Laboratory of Plant Virology, Fujian Agricultural and Forestry University, Jinshan, Fuzhou, 350002 Fujian, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Jia
authors.forename: Hongyan
authors.initials: H
authors.lastname: Chen
authors.forename: Qian
authors.initials: Q
authors.lastname: Chen
authors.forename: Lianhui
authors.initials: L
authors.lastname: Xie
authors.forename: Zujian
authors.initials: Z
authors.lastname: Wu
authors.forename: Taiyun
authors.initials: T
authors.lastname: Wei
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D008969:Molecular Sequence Data; D012275:Oryza / Q000821:virology*; D010935:Plant Diseases / Q000821:virology*; D017510:Protein Folding; D017434:Protein Structure, Tertiary; D012087:Reoviridae / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018411:Spodoptera / Q000821:virology*; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
chemical_list: D014764:Viral Proteins