title: Inhella fonticola sp. nov., isolated from spring water, and emended description of the genus Inhella.
abstract: A yellow-pigmented bacterial strain designated TNR-25(T) was isolated from spring water in Taiwan and was characterized using a polyphasic taxonomic approach. Strain TNR-25(T) was gram-negative, obligately aerobic, rod-shaped, non-motile and non-spore-forming. Growth occurred at 15-40 °C (optimum, 25 °C), at pH 6.0-10.0 (optimum, pH 7.0) and with 0-0.5 % NaCl (optimum, 0 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain TNR-25(T) belonged to the genus Inhella and its closest neighbour was Inhella inkyongensis IMCC1713(T) with 98.1 % sequence similarity. The major fatty acids (>10 %) of strain TNR-25(T) were summed feature 3 (comprising C(16 : 1)ω7c and/or C(16 : 1)ω6c) and C(16 : 0). The major cellular hydroxy fatty acids were C(10 : 0) 3-OH and C(12 : 0) 3-OH. The isoprenoid quinone was Q-8 and the DNA G+C content was 69.6 mol%. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylserine, diphosphatidylglycerol and several uncharacterized phospholipids. The DNA-DNA relatedness between strain TNR-25(T) and I. inkyongensis IMCC1713(T) was about 30.6-35.5 %. On the basis of the genotypic and phenotypic data, strain TNR-25(T) represents a novel species in the genus Inhella, for which the name Inhella fonticola sp. nov. is proposed; the type strain is TNR-25(T) ( = BCRC 80211(T) = LMG 25721(T)).
authors.affiliation: Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd, Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
authors.forename: Wen-Ming
authors.initials: WM
authors.lastname: Chen
authors.affiliation: Department of Marine Biotechnology, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd, Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
authors.forename: Fu-Sian
authors.initials: FS
authors.lastname: Sheu
authors.affiliation: College of Agriculture and Natural Resources, Department of Soil and Environmental Sciences, National Chung Hsing University, Taichung 402, Taiwan, ROC.
authors.forename: Chiu-Chung
authors.initials: CC
authors.lastname: Young
authors.affiliation: Department of Marine Biotechnology, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd, Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
authors.forename: Shih-Yi
authors.initials: SY
authors.lastname: Sheu
mesh_terms: D000332:Aerobiosis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D020562:Betaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D016000:Cluster Analysis; D003600:Cytosol / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D010860:Pigments, Biological / Q000378:metabolism; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D013624:Taiwan; D013696:Temperature; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D010860:Pigments, Biological; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride