title: [Role of mitochondrial calcium uniporter in cardioprotection induced by ischemic postconditioning in isolated rat heart].
abstract: To investigate the role of mitochondrial calcium uniporter in cardioprotection elicited by ischemic postconditioning (Postcond).
  
  
  
  Male Sprague-Dawley rats were used for Langendorff isolated heart perfusion. The hearts subjected to global ischemia for 30 min followed by 120 min of reperfusion. Left ventricular developed pressure (LVDP), maximal rise/fall rate of left ventricular pressure (± dP/dtmax) were measured. The level of lactate dehydrogenase (LDH) in the coronary effluent was measured spectrophotometrically, the content of formazan of myocardium was also measured at the end of reperfusion.
  
  
  
  Compared to I/R group, Postcond had an significant increase in the mechanical function of the left ventricle, with LDH release reduced and the content of formazan increased. Spermine, the opener of mitochondrial calcium uniporter, deteriorated the mechanical function of left ventricle and decreased the formazan content, and increased LDH release. Ruthenium red, the inhibitor of mitochondrial calcium uniporter, increased the mechanical function of the left ventricle, decreased the LDH release, but the content of formazan was not increased.
  
  
  
  The inhibition of mitochondrial calcium uniporter is involved in the mechanisms of ischemic postconditioning.
authors.affiliation: Department of Physiology, Zhejiang University, Hangzhou 310058, China.
authors.forename: Tu-nan
authors.initials: TN
authors.lastname: Yu
authors.forename: Hao
authors.initials: H
authors.lastname: Hong
authors.forename: Jie-qiong
authors.initials: JQ
authors.lastname: Yang
authors.forename: Qin
authors.initials: Q
authors.lastname: Gao
authors.forename: Qiang
authors.initials: Q
authors.lastname: Xia
mesh_terms: D000818:Animals; D015220:Calcium Channels / Q000378:metabolism / Q000502:physiology*; D004195:Disease Models, Animal; D005562:Formazans / Q000032:analysis; D006321:Heart / Q000503:physiopathology; D057775:Ischemic Postconditioning; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000378:metabolism / Q000503:physiopathology / Q000517:prevention & control*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D015220:Calcium Channels; D005562:Formazans; C034703:mitochondrial calcium uniporter; D007770:L-Lactate Dehydrogenase