title: PCR and the detection of Pseudomonas aeruginosa in respiratory samples of CF patients. A literature review.
abstract: Previous studies proved the importance of rapid antibacterial intervention in case of Pseudomonas aeruginosa detection in respiratory samples of cystic fibrosis patients. To improve the early detection of P. aeruginosa, several culture, PCR and serology based approaches have been compared. Because an increasing number of routine microbiology laboratories have access to real-time PCR (qPCR), we reviewed the specificity and sensitivity of published PCR formats. The importance of choice of DNA-extraction methods and PCR formats and of the validation of their specificity and sensitivity with clinical samples is stressed.
authors.affiliation: Laboratory Bacteriology Research, University of Ghent, De Pintelaan 185, 9000 Ghent, Belgium. Deschaght.Pieter@UGent.be
authors.forename: Pieter
authors.initials: P
authors.lastname: Deschaght
authors.forename: Sabine
authors.initials: S
authors.lastname: Van Daele
authors.forename: Frans
authors.initials: F
authors.lastname: De Baets
authors.forename: Mario
authors.initials: M
authors.lastname: Vaneechoutte
mesh_terms: D003550:Cystic Fibrosis / Q000382:microbiology*; D006801:Humans; D018410:Pneumonia, Bacterial / Q000175:diagnosis / Q000382:microbiology; D011552:Pseudomonas Infections / Q000175:diagnosis* / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000302:isolation & purification*; D060888:Real-Time Polymerase Chain Reaction