title: Neutrophil-derived resistin release induced by Aggregatibacter actinomycetemcomitans.
abstract: Resistin is an adipokine that induces insulin resistance in mice. In humans, resistin is not produced in adipocytes, but in various leukocytes instead, and it acts as a proinflammatory molecule. The present investigation demonstrated high levels of resistin in culture supernatants of neutrophils that are stimulated by a highly leukotoxic strain of Aggregatibacter actinomycetemcomitans. In contrast, the level of resistin was remarkably low when neutrophils were exposed to two other strains that produce minimal levels of leukotoxin and a further isogenic mutant strain incapable of producing leukotoxin. Pretreatment of neutrophils with a monoclonal antibody to CD18, β chain of lymphocyte function-associated molecule 1 (LFA-1), or an Src family tyrosine kinase inhibitor before incubation with the highly leukotoxic strain inhibited the release of resistin. These results show that A. actinomycetemcomitans-expressed leukotoxin induces extracellular release of human neutrophil-derived resistin by interacting with LFA-1 on the surface of neutrophils and, consequently, activating Src family tyrosine kinases.
authors.affiliation: Department of Oral Health, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
authors.forename: Reiko
authors.initials: R
authors.lastname: Furugen
authors.forename: Hideaki
authors.initials: H
authors.lastname: Hayashida
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Yoshii
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Saito
mesh_terms: D000704:Analysis of Variance; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D002478:Cells, Cultured; D004306:Dose-Response Relationship, Immunologic; D005098:Exotoxins / Q000276:immunology / Q000378:metabolism; D006801:Humans; D016169:Lymphocyte Function-Associated Antigen-1 / Q000378:metabolism; D009504:Neutrophils / Q000276:immunology* / Q000378:metabolism; D010196:Pancreatic Elastase / Q000378:metabolism; D015661:Pasteurellaceae / Q000276:immunology* / Q000378:metabolism; D052243:Resistin / Q000276:immunology* / Q000378:metabolism; D019061:src-Family Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D005098:Exotoxins; D016169:Lymphocyte Function-Associated Antigen-1; D052243:Resistin; C018579:leukotoxin; D019061:src-Family Kinases; D010196:Pancreatic Elastase