title: Expression of Nogo-66 receptor in human astrocytoma is correlated with tumor malignancy.
abstract: Nogo-A is a myelin-associated neuronal growth inhibitory molecule in central nervous system after trauma. However, the physiological functions of Nogo-A in neural development and in healthy oligodendrocytes are largely unknown. In this study, we investigated the expression of Nogo-66 receptor (NgR) protein in 60 cases of human astrocytoma by Western blot RT-PCR and immunohistochemistry. The correlation between the expression of NgR and pathologic grades of astrocyoma was further analyzed. The results showed that the expression of NgR protein and NgR mRNA immunoreactivity score, were decreased markedly with the increasing pathological grades. Double immunostaining results showed that Nogo-A and NgR were colocalized at the interface of astrocytoma cells and extracellular matrix. Our results indicated that NgR may have inhibitory effects on tumor activity and Nogo-A may restrict migration of tumor cells via NgR.
authors.affiliation: Department of Neurosurgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, People's Republic of China. mozhuoxiong@163.com
authors.forename: Nanxiang
authors.initials: N
authors.lastname: Xiong
authors.forename: Jianying
authors.initials: J
authors.lastname: Shen
authors.forename: Shuai
authors.initials: S
authors.lastname: Li
authors.forename: Junjun
authors.initials: J
authors.lastname: Li
authors.forename: Hongyang
authors.initials: H
authors.lastname: Zhao
mesh_terms: D000704:Analysis of Variance; D001254:Astrocytoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D015153:Blotting, Western; D002465:Cell Movement / Q000235:genetics / Q000502:physiology; D002681:China; D017931:DNA Primers / Q000235:genetics; D005109:Extracellular Matrix / Q000378:metabolism; D005260:Female; D058851:GPI-Linked Proteins / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D009185:Myelin Proteins / Q000378:metabolism*; D000070816:Nogo Receptor 1; D011956:Receptors, Cell Surface / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D058851:GPI-Linked Proteins; D009185:Myelin Proteins; D000070816:Nogo Receptor 1; C486580:RTN4R protein, human; D011956:Receptors, Cell Surface