title: [Simultaneous determination of seven nucleosides and nucleobases in aqueous extracts of Fritillaria taipaiensis by HPLC-DAD].
abstract: OBJECTIVE
  To develop a high performance liquid chromatography coupled with a photodiode array detector (HPLC-DAD) method for simultaneous determination of 7 nucleosides and nucleobases in Fritillaria taipaiensis.
  
  
  METHODS
  The analyses were performed on an Agilent Zorbax-SB-Aq-C18 column (4.6 mm x 250 mm, 5 microm) eluted with water and methanol in gradient mode. The flow rate was 1.0 mL x min(-1). The detection wavelength was 260 nm. The temperature of sample manager was set at 25 degrees C, and the injection volume was 20 microL.
  
  
  RESULTS
  The investigated compounds including uracil, cytidine, uridine, guanosine, thymidine, adenosine and adenine were shown good linearity (r > or = 0.999 8) over the tested ranges. The average recoveries were within 96.96% - 103.5% with RSD < or = 3.8%.
  
  
  CONCLUSIONS
  The accuracy, stability, repeatability and average recovery of the method are satisfying, and the seven nucleosides and nucleobases components in F. taipaiensis can be rapidly and accurately quantified by HPLC-DAD. This work provided helpful information for comprehensive quality evaluation of F. taipaiensis.
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.
authors.forename: Linfang
authors.initials: L
authors.lastname: Huang
authors.forename: Baozhong
authors.initials: B
authors.lastname: Duan
authors.forename: Lizhi
authors.initials: L
authors.lastname: Wang
authors.forename: Dahua
authors.initials: D
authors.lastname: Wei
authors.forename: Qifu
authors.initials: Q
authors.lastname: Lu
authors.forename: Shilin
authors.initials: S
authors.lastname: Chen
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D031410:Fritillaria / Q000737:chemistry*; D009705:Nucleosides / Q000032:analysis*; D015203:Reproducibility of Results; D014867:Water / Q000737:chemistry
chemical_list: D004365:Drugs, Chinese Herbal; D009705:Nucleosides; D014867:Water