title: The effect of doxycycline on atherogenesis in apoE-knockout mice.
abstract: Doxycycline at subantimicrobial doses inhibits matrix metalloproteinases (MMPs) activity, and is the only MMP inhibitor which is widely available in clinical practice. The aim of the study was to reveal whether non-specific MMPs inhibition by tetracycline could ameliorate development of atherosclerosis in apolipoprotein E (apoE)-knockout mice. Doxycycline (1.5 mg/ kg b.w./day) administered orally attenuated atherogenesis, measured both by "en face" method (10.25±1.7% vs. 15.7±2.0%, p<0.05) and "cross-section" method (66,254±7,468 μm(2) vs. 90,687±8,521 μm(2), p<0.05). In-situ zymography showed decrease of the extent of non-specific gelatinase activity in doxycycline-treated mice This is the first report to date describing the effect of doxycycline on atherogenesis in apoE-targeted mice.
authors.affiliation: Pharmacology, Jagiellonian University School of Medicine, 16 Grzegorzecka Street, Cracow, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Pawlowska
authors.forename: M
authors.initials: M
authors.lastname: Gajda
authors.forename: G
authors.initials: G
authors.lastname: Pyka-Fosciak
authors.forename: J
authors.initials: J
authors.lastname: Toton-Zuranska
authors.forename: A
authors.initials: A
authors.lastname: Niepsuj
authors.forename: K
authors.initials: K
authors.lastname: Kus
authors.forename: B
authors.initials: B
authors.lastname: Bujak-Gizycka
authors.forename: M
authors.initials: M
authors.lastname: Suski
authors.forename: R
authors.initials: R
authors.lastname: Olszanecki
authors.forename: J
authors.initials: J
authors.lastname: Jawien
authors.forename: R
authors.initials: R
authors.lastname: Korbut
mesh_terms: D000818:Animals; D001057:Apolipoproteins E / Q000172:deficiency*; D050197:Atherosclerosis / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism*; D004318:Doxycycline / Q000494:pharmacology / Q000627:therapeutic use*; D005260:Female; D061965:Matrix Metalloproteinase Inhibitors; D020782:Matrix Metalloproteinases / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout
chemical_list: D001057:Apolipoproteins E; D061965:Matrix Metalloproteinase Inhibitors; D020782:Matrix Metalloproteinases; D004318:Doxycycline