title: New cell separation technique for the isolation and analysis of cells from biological mixtures in forensic caseworks.
abstract: OBJECTIVE
  To isolate mucosal cells of the perpetrator in a sexual assault case from a complex mixture of his mucosal cells and the victim's skin by micromanipulation prior to genomic analysis.
  
  
  METHODS
  To capture and analyze mucosal cells we used the micromanipulation with on-chip low volume polymerase chain reaction (LV-PCR). Consensus DNA profiles were generated from 5 replicate experiments.
  
  
  CONCLUSIONS
  We validated the use of micromanipulation with on-chip LV-PCR for genomic analysis of complex biological mixtures in a fatal rape case. The perpetrator's mucosal cells were captured from nipple swabs of the victim, and a single-source DNA profile was generated from cell mixtures. These data suggest that micromanipulation with on-chip LV-PCR is an effective forensic tool for the analysis of specific cells from complex samples.
authors.affiliation: Institute of Forensic Science, Ministry of Public Security, Beijing, China.
authors.forename: Cai-xia
authors.initials: CX
authors.lastname: Li
authors.forename: Gui-qiang
authors.initials: GQ
authors.lastname: Wang
authors.forename: Wan-shui
authors.initials: WS
authors.lastname: Li
authors.forename: Jiang-ping
authors.initials: JP
authors.lastname: Huang
authors.forename: An-quan
authors.initials: AQ
authors.lastname: Ji
authors.forename: Lan
authors.initials: L
authors.lastname: Hu
mesh_terms: D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D003415:Crime; D004247:DNA / Q000032:analysis; D004586:Electrophoresis; D044707:Forensic Sciences / Q000295:instrumentation / Q000379:methods*; D005819:Genetic Markers; D005838:Genotype; D006708:Homicide / Q000706:statistics & numerical data*; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics*; D011902:Rape / Q000706:statistics & numerical data*
chemical_list: D005819:Genetic Markers; D004247:DNA