title: Controlled density patterning of tolylterpyridine-tagged oligonucleotides.
abstract: An efficient surface anchoring strategy of tolylterpyridine-tagged DNA single strands (ssDNA-ttpy) synthesized on gold electrodes is reported. The method is based on exchange reactions between Fe(II)bis-terpyridine complexed SAMs and ssDNA-ttpy, and allows efficient hybrydization of the cDNA strands. Moreover, by using low-current focused ion beam lithography, micropatterned arrays are obtained, homogeneously covered with ssDNA-ttpy. The surface adsorption kinetics of ssDNA-ttpy, as well as its hybridization efficiency, was monitored by in situ quartz crystal microbalance with dissipation monitoring (QCM-D) technique. The effective confinement of the ssDNA-ttpy at the micrometer level has been monitored by time of flight secondary ion mass spectrometry (ToF-SIMS) and ellipsometric surface imaging experiments, providing laterally resolved chemical and topographic mapping.
authors.affiliation: Laboratory for Molecular Surfaces and Nanotechnology (LAMSUN), Dipartimento di Scienze Chimiche, Università degli Studi di Catania and CSGI, Catania, Italy.
authors.forename: Nunzio
authors.initials: N
authors.lastname: Tuccitto
authors.forename: Nicoletta
authors.initials: N
authors.lastname: Giamblanco
authors.forename: Sumana
authors.initials: S
authors.lastname: Ghosh
authors.forename: Valentina
authors.initials: V
authors.lastname: Spampinato
authors.forename: Pierre
authors.initials: P
authors.lastname: Labbé
authors.forename: Pascal
authors.initials: P
authors.lastname: Dumy
authors.forename: Silvio
authors.initials: S
authors.lastname: Quici
authors.forename: Giovanni
authors.initials: G
authors.lastname: Marletta
authors.forename: Eric
authors.initials: E
authors.lastname: Defrancq
authors.forename: Antonino
authors.initials: A
authors.lastname: Licciardello
mesh_terms: D001483:Base Sequence; D015342:DNA Probes / Q000737:chemistry; D004277:DNA, Single-Stranded / Q000737:chemistry* / Q000235:genetics; D004566:Electrodes; D006046:Gold / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000737:chemistry* / Q000235:genetics; D011725:Pyridines / Q000737:chemistry*
chemical_list: D015342:DNA Probes; D004277:DNA, Single-Stranded; D009838:Oligodeoxyribonucleotides; D011725:Pyridines; D006046:Gold