title: Immunoglobulin G antibody profiles against Anopheles salivary proteins in domestic animals in Senegal.
abstract: Although domestic animals may not be permissive for Plasmodium, they could nevertheless play a role in the epidemiology of malaria by attracting Anopheles away from humans. To investigate interactions between domestic animals and mosquitoes, we assayed immunoglobulin G (IgG) antibodies directed against the salivary proteins of Anopheles gambiae in domestic animals living in Senegalese villages where malaria is endemic. By Western blotting, sera from bovines (n=6), ovines (n=36), and caprines (n=36) did not react with Anopheles whole saliva. In contrast, equine sera recognized proteins in both saliva and salivary gland extracts. Two of the major immunogens (32 and 72 kDa) were also reactive in extracts from other major mosquito genera (Aedes and Culex), but reactions toAnopheles-specific antigens were detected in 12 of 17 horses. These data suggest that horses strongly react to Anopheles bites, and further experiments on horses are warranted to investigate the impact of this domestic animal species on the transmission of human malaria.
authors.affiliation: Institut de Recherche pour le Développement, UMR 145, 911 avenue Agropolis, BP64501, 34394 Montpellier, France. denis.boulanger@ird.fr
authors.forename: Denis
authors.initials: D
authors.lastname: Boulanger
authors.forename: Souleymane
authors.initials: S
authors.lastname: Doucoure
authors.forename: Lise
authors.initials: L
authors.lastname: Grout
authors.forename: Abdoulaye
authors.initials: A
authors.lastname: Ngom
authors.forename: François
authors.initials: F
authors.lastname: Rogerie
authors.forename: Sylvie
authors.initials: S
authors.lastname: Cornelie
authors.forename: Cheikh
authors.initials: C
authors.lastname: Sokhna
authors.forename: François
authors.initials: F
authors.lastname: Mouchet
authors.forename: Gilles
authors.initials: G
authors.lastname: Riveau
authors.forename: François
authors.initials: F
authors.lastname: Simondon
authors.forename: Franck J
authors.initials: FJ
authors.lastname: Remoue
mesh_terms: D000818:Animals; D000829:Animals, Domestic / Q000276:immunology*; D000852:Anopheles / Q000276:immunology*; D000953:Antigens, Protozoan / Q000276:immunology*; D015153:Blotting, Western; D005260:Female; D007074:Immunoglobulin G / Q000097:blood* / Q000276:immunology; D019476:Insect Proteins / Q000276:immunology; D012471:Salivary Proteins and Peptides / Q000276:immunology*; D012675:Senegal; D013045:Species Specificity
chemical_list: D000953:Antigens, Protozoan; D007074:Immunoglobulin G; D019476:Insect Proteins; D012471:Salivary Proteins and Peptides