title: The effects of different preservation processes on the total protein and growth factor content in a new biological product developed from human amniotic membrane.
abstract: The aim of this work is to quantify the total protein and growth factors content in a tissue-suspension obtained from processed human amniotic membrane (hAM). hAM was collected, frozen, freeze dried, powdered and sterilized by γ-irradiation. At each step of the process, samples were characterized for the total protein amounts by a Bradford protein assay and for the growth factor concentrations by ELISA test of the tissue suspensions. Frozen-hAM samples show higher release of total proteins and specific growth factors in the tissue suspension in comparison with freeze-dried hAM. We observed that even if the protein extraction is hindered once the tissue is dried, the powdering process allows a greater release in the tissue suspension of total proteins and growth factors after tissue re-solubilization in comparison with only the freeze-drying process (+91 ± 13% for EGF, +16 ± 4% for HGF, +11 ± 5% for FGF, +16 ± 9% for TGF-β1), and a greater release of EGF (85 ± 10%) in comparison with only the freezing process, because proteins become much readily solubilized in the solution. According with these results, we describe a protocol to obtain a new sterile biological product from hAM tissue, with well-known effects of thermal, mechanical and physical processes on the total protein and grow factors contents.
authors.affiliation: Eye Bank, Department of Ophthalmology, S. Maria della Scaletta Hospital, Via Montericco 4, Imola, Bologna, Italy. banca.occhi@ausl.imola.bo.it
authors.forename: Alessandra
authors.initials: A
authors.lastname: Russo
authors.forename: Paola
authors.initials: P
authors.lastname: Bonci
authors.forename: Paolo
authors.initials: P
authors.lastname: Bonci
mesh_terms: D000650:Amnion / Q000378:metabolism*; D001688:Biological Products / Q000378:metabolism*; D005260:Female; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D011309:Preservation, Biological / Q000379:methods*; D011506:Proteins / Q000378:metabolism*
chemical_list: D001688:Biological Products; D036341:Intercellular Signaling Peptides and Proteins; D011506:Proteins