title: Biotechnological applications of recombinant single-domain antibody fragments.
abstract: BACKGROUND
  Single-domain antibody fragments possess structural features, such as a small dimension, an elevated stability, and the singularity of recognizing epitopes non-accessible for conventional antibodies that make them interesting for several research and biotechnological applications.
  
  
  RESULTS
  The discovery of the single-domain antibody's potentials has stimulated their use in an increasing variety of fields. The rapid accumulation of articles describing new applications and further developments of established approaches has made it, therefore, necessary to update the previous reviews with a new and more complete summary of the topic.
  
  
  CONCLUSIONS
  Beside the necessary task of updating, this work analyses in detail some applicative aspects of the single-domain antibodies that have been overseen in the past, such as their efficacy in affinity chromatography, as co-crystallization chaperones, protein aggregation controllers, enzyme activity tuners, and the specificities of the unconventional single-domain fragments.
authors.affiliation: University of Nova Gorica (UNG), Vipavska 13, PO Box 301-SI-5000, Rožna Dolina (Nova Gorica), Slovenia. ario.demarco@ung.si
authors.forename: Ario
authors.initials: A
authors.lastname: de Marco
mesh_terms: D001419:Bacteria / Q000276:immunology; D001435:Bacteriophages / Q000276:immunology; D001709:Biotechnology; D005658:Fungi / Q000276:immunology; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D057127:Single-Chain Antibodies / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D014118:Toxins, Biological / Q000276:immunology; D014780:Viruses / Q000276:immunology
chemical_list: D011994:Recombinant Proteins; D057127:Single-Chain Antibodies; D014118:Toxins, Biological