title: [From genetics to genomics in the rational design of new Mycobacterium tuberculosis vaccines].
abstract: Tuberculosis (TB) is an infectious disease affecting people from all ages all over the world. It is estimated that one third of the world population lives infected with the causal agent: Mycobacterium tuberculosis. Despite availability and systematic administration of BCG vaccine in endemic areas, TB transmission remains elusive to control, partly because BGC efficacy has been shown to have wide variability (0-80%). Such variability in protection is attributed to factors including: the BCG strain used for immunization, pre-existing exposure to environmental saprophytic Mycobacterium species, and host genetic factors. In this context, efforts regarding to re-engineering BCG vaccines with the ability to prevent latent TB reactivation, providing long lasting protection, and devoid from collateral effects in immunosuppressed people are urgent. In this work we review the actual molecular «gene-by-gene» strategies aimed at generating BCG alternatives, and discuss the urgent necessity of high throughput technology methods for a rational design for a new TB vaccine.
authors.affiliation: Departamento de Biología Molecular y Bioingeniería, UAM Reynosa-Aztlán, Universidad Autónoma de Tamaulipas, Tamaulipas, México.
authors.forename: Virgilio
authors.initials: V
authors.lastname: Bocanegra-García
authors.forename: Jorge
authors.initials: J
authors.lastname: Valencia-Delgadillo
authors.forename: Wendy
authors.initials: W
authors.lastname: Cruz-Pulido
authors.forename: Rubén
authors.initials: R
authors.lastname: Cantú-Ramírez
authors.forename: Gildardo
authors.initials: G
authors.lastname: Rivera-Sánchez
authors.forename: José Prisco
authors.initials: JP
authors.lastname: Palma-Nicolás
mesh_terms: D000942:Antigens, Bacterial / Q000276:immunology; D001500:BCG Vaccine; D004269:DNA, Bacterial / Q000235:genetics / Q000276:immunology; D015195:Drug Design; D000939:Epitopes / Q000276:immunology; D005798:Genes, Bacterial; D005822:Genetic Vectors / Q000235:genetics / Q000276:immunology; D023281:Genomics; D057166:High-Throughput Screening Assays; D006801:Humans; D009163:Mycobacterium bovis / Q000235:genetics / Q000276:immunology; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000276:immunology*; D032581:Tuberculosis Vaccines; D019444:Vaccines, DNA; D022223:Vaccines, Subunit; D014614:Vaccines, Synthetic
chemical_list: D000942:Antigens, Bacterial; D001500:BCG Vaccine; D004269:DNA, Bacterial; D000939:Epitopes; D032581:Tuberculosis Vaccines; D019444:Vaccines, DNA; D022223:Vaccines, Subunit; D014614:Vaccines, Synthetic