title: EMF1 interacts with EIP1, EIP6 or EIP9 involved in the regulation of flowering time in Arabidopsis.
abstract: The EMBRYONIC FLOWER (EMF) 1 gene has been shown to be necessary for maintenance of vegetative development. To investigate the molecular mechanism of EMF1-mediated plant development, we screened EMF1-interacting proteins and identified 11 candidate proteins using the yeast two-hybrid system. Among the candidate genes, three EMF1-Interacting Protein (EIP) genes, EIP1, EIP6 and EIP9, are predicted to encode a WNK (with-no-lysine) kinase, a B-box zinc-finger protein and a DnaJ-domain protein, respectively. The expression patterns of EIP1, EIP6 and EIP9 were similar to that of EMF1, and EMF1-EIP1, EMF1-EIP6 and EMF1-EIP9 heterodimers were localized in the nucleus. In addition, eip1, eip6 and eip9 mutants flowered early and showed increased expression of flowering-time and floral organ identity genes, while EIP1-, EIP6- and EIP9-overexpressing transgenic plants showed late flowering phenotypes. Our results suggest that EMF1 interacts with EIP1, EIP6 and EIP9 during vegetative development to regulate flowering time in Arabidopsis.
authors.affiliation: Department of Molecular Biology, Pusan National University, Busan, 609-735, Korea.
authors.forename: Hee-Yeon
authors.initials: HY
authors.lastname: Park
authors.forename: Sun-Young
authors.initials: SY
authors.lastname: Lee
authors.forename: Hye-Yeon
authors.initials: HY
authors.lastname: Seok
authors.forename: Sun-Ho
authors.initials: SH
authors.lastname: Kim
authors.forename: Z Renee
authors.initials: ZR
authors.lastname: Sung
authors.forename: Yong-Hwan
authors.initials: YH
authors.lastname: Moon
mesh_terms: D017360:Arabidopsis / Q000166:cytology / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D035264:Flowers / Q000235:genetics / Q000502:physiology*; D018506:Gene Expression Regulation, Plant; D016254:Mutagenesis, Insertional / Q000235:genetics; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D030821:Plants, Genetically Modified; D011485:Protein Binding; D025941:Protein Interaction Mapping; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D013347:Subcellular Fractions / Q000378:metabolism; D013997:Time Factors; D020798:Two-Hybrid System Techniques; D016335:Zinc Fingers
chemical_list: D029681:Arabidopsis Proteins; D002352:Carrier Proteins; D004269:DNA, Bacterial; C435921:EMF1 protein, Arabidopsis; C036483:T-DNA; C568340:EIP1 protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases