title: Immunochromatographic test with recombinant Em18 antigen for the follow-up study of alveolar echinococcosis.
abstract: The performance of a rapid and simple immunochromatographic test (ICT) with recombinant Em18 (rEm18) antigen for serological follow-up of Echinococcus multilocularis infection was evaluated by comparison with that of an enzyme-linked immunosorbent assay (ELISA) with rEm18, using serum samples from patients who underwent surgery and/or received antiparasitic chemotherapy. The degree of Em18-band intensity on the ICT correlated highly with the absorbance value obtained by the ELISA. The kinetics of antibody levels obtained by the ICT paralleled those of the ELISA. These data suggest that the ICT has high potential as an easy-to-handle, fast, and reliable follow-up tool to monitor the status of alveolar echinococcosis in different stages.
authors.affiliation: Department of Parasitology, Asahikawa Medical University, Midorigaoka Higashi 2-1-1-1, Asahikawa, 078-8510 Hokkaido, Japan. yasusako@asahikawa-med.ac.jp
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Sako
authors.forename: Dennis
authors.initials: D
authors.lastname: Tappe
authors.forename: Kenta
authors.initials: K
authors.lastname: Fukuda
authors.forename: Yukuharu
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: Sonoyo
authors.initials: S
authors.lastname: Itoh
authors.forename: Matthias
authors.initials: M
authors.lastname: Frosch
authors.forename: Beate
authors.initials: B
authors.lastname: Grüner
authors.forename: Peter
authors.initials: P
authors.lastname: Kern
authors.forename: Akira
authors.initials: A
authors.lastname: Ito
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D000947:Antigens, Helminth / Q000235:genetics; D000977:Antiparasitic Agents / Q000627:therapeutic use; D019411:Clinical Laboratory Techniques / Q000379:methods*; D016903:Drug Monitoring / Q000379:methods; D004443:Echinococcosis; D004444:Echinococcosis, Hepatic / Q000175:diagnosis* / Q000188:drug therapy / Q000601:surgery; D048210:Echinococcus multilocularis / Q000276:immunology*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007118:Immunoassay / Q000379:methods; D008297:Male; D008875:Middle Aged; D011994:Recombinant Proteins / Q000235:genetics; D055815:Young Adult
chemical_list: D000913:Antibodies, Protozoan; D000947:Antigens, Helminth; D000977:Antiparasitic Agents; C117889:Em18 antigen; D011994:Recombinant Proteins