title: Novel functions of lipid-binding protein 5 in Caenorhabditis elegans fat metabolism.
abstract: The lipid-binding protein (LBP) family is conserved from Caenorhabditis elegans to mammals and essential for fatty acid homeostasis. RNAi-mediated knockdown of nine C. elegans lbp family members revealed that lbp-5 regulates fat accumulation. C. elegans LBP-5 bound directly to various fatty acids with varying affinities. lbp-5 expression in nhr-49(nr2041) worms was much lower than in N2 worms. Nhr-49 transcriptional activity also decreased with lbp-5 deletion, suggesting that they may work together as functional partners in fat metabolism. In support of this notion, LBP-5 translocated into nuclei, where it appeared to influence C. elegans NHR-49 target genes involved in energy metabolism. Interestingly, LBP-5 is required for stearic acid-induced transcription of NHR-49 target genes. Thus, this knowledge could help identify therapeutic targets to treat obesity and diseases associated with nematode-host interactions.
authors.affiliation: Department of Biochemistry and Biomedical Science, World Class University Program, College of Life Science and Biotechnology, Yonsei Proteome Research Center, Yonsei University, Seoul 120-749, Korea.
authors.forename: Mo
authors.initials: M
authors.lastname: Xu
authors.forename: Hyoe-Jin
authors.initials: HJ
authors.lastname: Joo
authors.forename: Young-Ki
authors.initials: YK
authors.lastname: Paik
mesh_terms: D021581:Active Transport, Cell Nucleus / Q000502:physiology; D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism*; D004734:Energy Metabolism / Q000502:physiology; D050556:Fatty Acid-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D050356:Lipid Metabolism / Q000502:physiology*; D011485:Protein Binding; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics / Q000378:metabolism; D013229:Stearic Acids / Q000378:metabolism*; D014158:Transcription, Genetic / Q000502:physiology*
chemical_list: D029742:Caenorhabditis elegans Proteins; D002352:Carrier Proteins; D050556:Fatty Acid-Binding Proteins; C505159:NHR-49 protein, C elegans; D018160:Receptors, Cytoplasmic and Nuclear; D013229:Stearic Acids; C571931:lipid-binding protein 5, C elegans; C031183:stearic acid