title: Development of a 16S rRNA gene primer and PCR-restriction fragment length polymorphism method for rapid detection of members of the genus Megasphaera and species-level identification.
abstract: The genus Megasphaera is relevant to the environment, human health and food, and renewable energy for the future. In this study, a primer set was designed for PCR-restriction fragment length polymorphism (RFLP) analyses to detect and identify the members of Megasphaera. Direct detection and identification were achieved for environmental samples and isolates.
authors.affiliation: Akihiro Ohnishi, Department of Fermentation Science, Faculty of Applied Bio-Science, Tokyo University of Agriculture, 1-1 Sakuragaoka 1-chome, Setagaya-ku, Tokyo 156-8502, Japan. a1ohnish@nodai.ac.jp
authors.forename: Akihiro
authors.initials: A
authors.lastname: Ohnishi
authors.forename: Shinko
authors.initials: S
authors.lastname: Abe
authors.forename: Shiho
authors.initials: S
authors.lastname: Nashirozawa
authors.forename: Sayaka
authors.initials: S
authors.lastname: Shimada
authors.forename: Naoshi
authors.initials: N
authors.lastname: Fujimoto
authors.forename: Masaharu
authors.initials: M
authors.lastname: Suzuki
mesh_terms: D017931:DNA Primers / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D045851:Megasphaera / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000235:genetics*; D016415:Sequence Alignment
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S