title: Novel stem/progenitor cell population from murine tracheal submucosal gland ducts with multipotent regenerative potential.
abstract: The airway epithelium is in direct contact with the environment and therefore constantly at risk for injury. Basal cells (BCs) have been found to repair the surface epithelium (SE), but the contribution of other stem cell populations to airway epithelial repair has not been identified. We demonstrated that airway submucosal gland (SMG) duct cells, in addition to BCs, survived severe hypoxic-ischemic injury. We developed a method to isolate duct cells from the airway. In vitro and in vivo models were used to compare the self-renewal and differentiation potential of duct cells and BCs. We found that only duct cells were capable of regenerating SMG tubules and ducts, as well as the SE overlying the SMGs. SMG duct cells are therefore a multipotent stem cell for airway epithelial repair This is of importance to the field of lung regeneration as determining the repairing cell populations could lead to the identification of novel therapeutic targets and cell-based therapies for patients with airway diseases.
authors.affiliation: Department of Pediatrics, David Geffen School of Medicine at University of California Los Angeles, University of California Los Angeles School of Medicine, Los Angeles, California, USA.
authors.forename: Ahmed E
authors.initials: AE
authors.lastname: Hegab
authors.forename: Vi Luan
authors.initials: VL
authors.lastname: Ha
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Gilbert
authors.forename: Kelvin Xi
authors.initials: KX
authors.lastname: Zhang
authors.forename: Stephen P
authors.initials: SP
authors.lastname: Malkoski
authors.forename: Andy T
authors.initials: AT
authors.lastname: Chon
authors.forename: Daphne O
authors.initials: DO
authors.lastname: Darmawan
authors.forename: Bharti
authors.initials: B
authors.lastname: Bisht
authors.forename: Aik T
authors.initials: AT
authors.lastname: Ooi
authors.forename: Matteo
authors.initials: M
authors.lastname: Pellegrini
authors.forename: Derek W
authors.initials: DW
authors.lastname: Nickerson
authors.forename: Brigitte N
authors.initials: BN
authors.lastname: Gomperts
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D019070:Cell Lineage; D002469:Cell Separation; D058948:Cell Tracking; D002478:Cells, Cultured; D004848:Epithelium / Q000473:pathology; D020869:Gene Expression Profiling; D000860:Hypoxia / Q000473:pathology; D007511:Ischemia / Q000473:pathology; D053547:Keratin-14 / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D039902:Multipotent Stem Cells / Q000378:metabolism / Q000473:pathology* / Q000637:transplantation; D020411:Oligonucleotide Array Sequence Analysis; D012038:Regeneration; D020545:Respiratory Mucosa / Q000473:pathology*; D014132:Trachea / Q000098:blood supply / Q000473:pathology* / Q000503:physiopathology
chemical_list: D053547:Keratin-14