title: The different epidemiologic subtypes of Burkitt lymphoma share a homogenous micro RNA profile distinct from diffuse large B-cell lymphoma.
abstract: Sporadic Burkitt lymphoma (sBL) can be delineated from diffuse large B-cell lymphoma (DLBCL) by a very homogeneous mRNA expression signature. However, it remained unclear whether all three BL variants-sBL, endemic BL (eBL) and human immunodeficiency virus-associated BL (HIV-BL)-represent a uniform biological entity despite their differences in geographical occurrence, association with immunodeficiency and/or incidence of Epstein-Barr virus (EBV) infection. To address this issue, we generated micro RNA (miRNA) profiles from 18 eBL, 31 sBL and 15 HIV-BL cases. In addition, we analyzed the miRNA expression of 86 DLBCL to determine whether miRNA profiles recapitulate the molecular differences between BL and DLBCL evidenced by mRNA profiling. A signature of 38 miRNAs containing MYC regulated and nuclear factor-kB pathway-associated miRNAs was obtained that differentiated BL from DLBCL. The miRNA profiles of sBL and eBL displayed only six differentially expressed miRNAs, whereas HIV and EBV infection had no impact on the miRNA profile of BL. In conclusion, miRNA profiling confirms that BL and DLBCL represent distinct lymphoma categories and demonstrates that the three BL variants are representatives of the same biological entity with only marginal miRNA expression differences between eBL and sBL.
authors.affiliation: Institute of Pathology, Campus Benjamin Franklin, Charité-Universitätsmedizin Berlin, Berlin, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Lenze
authors.affiliation: Department of Human Pathology and Oncology, University of Siena, Siena, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Leoncini
authors.affiliation: Institute of Pathology, Campus Benjamin Franklin, Charité-Universitätsmedizin Berlin, Berlin, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Hummel
authors.affiliation: Department of Molecular Virology, Immunology and Medical Genetics and Comprehensive Cancer Center, Ohio State University, Columbus, OH, USA.
authors.forename: S
authors.initials: S
authors.lastname: Volinia
authors.affiliation: Department of Molecular Virology, Immunology and Medical Genetics and Comprehensive Cancer Center, Ohio State University, Columbus, OH, USA.
authors.forename: C G
authors.initials: CG
authors.lastname: Liu
authors.affiliation: Department of Human Pathology and Oncology, University of Siena, Siena, Italy.
authors.forename: T
authors.initials: T
authors.lastname: Amato
authors.affiliation: Department of Human Pathology and Oncology, University of Siena, Siena, Italy.
authors.forename: G
authors.initials: G
authors.lastname: De Falco
authors.affiliation: Department of Human Pathology, University of Nairobi, Nairobi, Kenya.
authors.forename: J
authors.initials: J
authors.lastname: Githanga
authors.affiliation: Dr Margarete Fischer-Bosch-Institute of Clinical Pharmacology, Würzburg, Stuttgart, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Horn
authors.affiliation: Department of Human Pathology, University of Nairobi, Nairobi, Kenya.
authors.forename: J
authors.initials: J
authors.lastname: Nyagol
authors.affiliation: Department of Clinical Pathology, Robert-Bosch-Hospital, Stuttgart, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Ott
authors.affiliation: Department of Molecular Virology, Immunology and Medical Genetics and Comprehensive Cancer Center, Ohio State University, Columbus, OH, USA.
authors.forename: J
authors.initials: J
authors.lastname: Palatini
authors.affiliation: Innere Medizin I, Saarland University, Homburg, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Pfreundschuh
authors.affiliation: Department of Human Pathology, University of Nairobi, Nairobi, Kenya.
authors.forename: E
authors.initials: E
authors.lastname: Rogena
authors.affiliation: Institute of Pathology, University of Würzburg, Würzburg, Germany, Campus Kiel, Kiel, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Rosenwald
authors.affiliation: Institute of Human Genetics, Christian-Albrechts-University Kiel and University Hospital Schleswig-Holstein, Campus Kiel, Kiel, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Siebert
authors.affiliation: Department of Molecular Virology, Immunology and Medical Genetics and Comprehensive Cancer Center, Ohio State University, Columbus, OH, USA.
authors.forename: C M
authors.initials: CM
authors.lastname: Croce
authors.affiliation: Institute of Pathology, Campus Benjamin Franklin, Charité-Universitätsmedizin Berlin, Berlin, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Stein
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D002051:Burkitt Lymphoma / Q000453:epidemiology / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D005858:Germany / Q000453:epidemiology; D006801:Humans; D007124:Immunoenzyme Techniques; D007558:Italy / Q000453:epidemiology; D007630:Kenya / Q000453:epidemiology; D016403:Lymphoma, Large B-Cell, Diffuse / Q000453:epidemiology / Q000235:genetics* / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs; D012333:RNA, Messenger