title: Serum paraoxonase enzyme activity and oxidative stress in obese subjects.
abstract: BACKGROUND
  Obesity is an important risk factor for atherosclerotic cardiovascular disease. Paraoxonase-1 (PON1) may play a significant role in the prevention of obesity-related accelerated atherosclerosis by hydrolyzing lipid peroxides in oxidized low-density lipoproteins.
  
  
  OBJECTIVE
  The aim of this study was to evaluate PON1 and arylesterase enzyme activities and lipid hydroperoxide (LOOH) levels, and to investigate whether there is increased susceptibility to atherogenesis in obese subjects, which might be reflected by increased oxidative stress and decreased PON1 activity. We also aimed to investigate the association between PON1 activity and body mass index (BMI) in this patient group.
  
  
  METHODS
  The study involved 25 obese subjects and 23 controls. Serum PON1 and arylesterase activity was measured spectrophotometrically. LOOH levels were measured by the FOX-2 assay.
  
  
  RESULTS
  Serum basal/salt-stimulated PON1 and arylesterase activities were significantly lower in obese subjects than in controls (P <0.001 for both enzymes), while LOOH levels were significantly higher (P <0.001). BMI was significantly correlated with PON1, arylesterase and LOOH levels (P <0.001, r = -0.720; P <0.001, r = -0.634; P <0.001, r = 0.491; respectively). Serum high-density lipoprotein (HDL) levels were positively correlated with PON1 activity (r = 0.347, P <0.05).
  
  
  CONCLUSIONS
  Our results indicate that obese subjects have increased oxidative stress and decreased PON1 activity, which might contribute to accelerated atherosclerosis. A decrease in PON1 activity seems positively correlated with BMI and inversely correlated with HDL levels.
authors.affiliation: Ozel Ercis Capa Medicine Center, Internal Medicine Clinic, Van, Turkey. m.aslan301@mynet.com
authors.forename: Mehmet
authors.initials: M
authors.lastname: Aslan
authors.forename: Mehmet
authors.initials: M
authors.lastname: Horoz
authors.forename: Tevfik
authors.initials: T
authors.lastname: Sabuncu
authors.forename: Hakim
authors.initials: H
authors.lastname: Celik
authors.forename: Sahbettin
authors.initials: S
authors.lastname: Selek
mesh_terms: D000328:Adult; D043303:Aryldialkylphosphatase / Q000378:metabolism*; D050197:Atherosclerosis / Q000201:enzymology*; D015992:Body Mass Index; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D008054:Lipid Peroxides / Q000378:metabolism; D008075:Lipoproteins, HDL / Q000097:blood; D008297:Male; D009765:Obesity / Q000201:enzymology*; D018384:Oxidative Stress; D012307:Risk Factors
chemical_list: D008054:Lipid Peroxides; D008075:Lipoproteins, HDL; D002265:Carboxylic Ester Hydrolases; C019734:arylesterase; D043303:Aryldialkylphosphatase