title: Preparation of zwitterionic polymeric monolithic columns for hydrophilic interaction capillary liquid chromatography.
abstract: Porous zwitterionic monolithic columns based on photo-initiated copolymerization of N,N-dimethyl-N-methacryloxyethyl-N-(3-sulfopropyl)ammonium betaine and poly(ethylene glycol) diacrylate in a binary porogen system comprising isopropanol and decanol were prepared in 75-μm-id fused silica capillaries. The resulting monolith was evaluated by hydrophilic interaction chromatography. Inverse size-exclusion chromatography was used to characterize the pore structure of the resulting monolith. A typical hydrophilic interaction chromatography mechanism was observed when the organic content in the mobile phase was higher than 60%. Good separations of amides, phenols, and benzoic acids were achieved. An efficiency of 75,000 plates/m was obtained. The effects of mobile phase pH, salt concentration, and organic modifier content on retention were investigated. For polar-charged analytes, both hydrophilic interactions and electrostatic interactions contributed to the selectivity.
authors.affiliation: Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT, USA.
authors.forename: Xin
authors.initials: X
authors.lastname: Chen
authors.forename: H Dennis
authors.initials: HD
authors.lastname: Tolley
authors.forename: Milton L
authors.initials: ML
authors.lastname: Lee
mesh_terms: D002853:Chromatography, Liquid / Q000295:instrumentation* / Q000379:methods; D057927:Hydrophobic and Hydrophilic Interactions; D058105:Polymerization; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D016062:Porosity; D055672:Static Electricity
chemical_list: D011108:Polymers