title: Rapid titration of viruses by flow cytometry.
abstract: Traditionally, the most common methods used to titrate virus stocks are the plaque assay and the hemagglutination assay. The protocol presented here is based on the detection of viral-expressed proteins in infected cells by flow cytometry. It is simpler and more rapid than the traditional plaque-forming assay and it enables high-throughput analyses.
authors.affiliation: Department of Hematology, Hadassah Medical School, The Hebrew University of Jerusalem, Jerusalem, Israel.
authors.forename: Nir
authors.initials: N
authors.lastname: Drayman
authors.forename: Ariella
authors.initials: A
authors.lastname: Oppenheim
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D002460:Cell Line; D005434:Flow Cytometry / Q000379:methods*; D013539:Simian virus 40 / Q000302:isolation & purification; D019562:Viral Load / Q000379:methods*; D014780:Viruses / Q000302:isolation & purification*
chemical_list: D000914:Antibodies, Viral