title: Use of antistaphylococcal beta-lactams to increase daptomycin activity in eradicating persistent bacteremia due to methicillin-resistant Staphylococcus aureus: role of enhanced daptomycin binding.
abstract: We used daptomycin plus antistaphylococcal β-lactams (ASBL) to clear refractory MRSA bacteremia. In vitro studies showed enhanced daptomycin bactericidal activity, increased membrane daptomycin binding, and decrease in positive surface charge induced by ASBLs against daptomycin nonsusceptible MRSA. Addition of ASBLs to daptomycin may be of benefit in refractory MRSA bacteremia. (Although the official designation is "daptomycin nonsusceptiblity," we will use the term "daptomycin-resistance" in this paper for facility of presentation.).
authors.affiliation: Department of Medicine, Division of Infectious Diseases, New York Medical College, Valhalla, New York 10595, USA. abhay_dhand@nymc.edu
authors.forename: Abhay
authors.initials: A
authors.lastname: Dhand
authors.forename: Arnold S
authors.initials: AS
authors.lastname: Bayer
authors.forename: Joseph
authors.initials: J
authors.lastname: Pogliano
authors.forename: Soo-Jin
authors.initials: SJ
authors.lastname: Yang
authors.forename: Michael
authors.initials: M
authors.lastname: Bolaris
authors.forename: Victor
authors.initials: V
authors.lastname: Nizet
authors.forename: Guiquing
authors.initials: G
authors.lastname: Wang
authors.forename: George
authors.initials: G
authors.lastname: Sakoulas
mesh_terms: D000328:Adult; D000369:Aged, 80 and over; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000494:pharmacology; D016470:Bacteremia / Q000188:drug therapy* / Q000382:microbiology*; D002462:Cell Membrane / Q000737:chemistry / Q000502:physiology; D017576:Daptomycin / Q000008:administration & dosage* / Q000494:pharmacology; D024881:Drug Resistance, Bacterial; D004359:Drug Therapy, Combination / Q000379:methods; D005260:Female; D006801:Humans; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects / Q000302:isolation & purification*; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D016896:Treatment Outcome; D047090:beta-Lactams / Q000008:administration & dosage* / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D047090:beta-Lactams; D017576:Daptomycin