title: CdSe/ZnS quantum dot-Cytochrome c bioconjugates for selective intracellular O2˙⁻ sensing.
abstract: We demonstrate that the coupling system of negatively capped CdSe/ZnS QDs with an oxidized Cytochrome c (Cyt c) is capable of the fluorescent imaging of a superoxide radical (O(2)˙⁻) with high sensitivity and specificity in living cells, without interference from other Reactive Oxygen Species (ROS) or relevant intracellular components.
authors.affiliation: Shanghai Key Laboratory of Functional Materials Chemistry & Department of Chemistry, East China University of Science and Technology, 130 Meilong Road, Shanghai, PR China.
authors.forename: Da-Wei
authors.initials: DW
authors.lastname: Li
authors.forename: Li-Xia
authors.initials: LX
authors.lastname: Qin
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.forename: Raheleh Partovi
authors.initials: RP
authors.lastname: Nia
authors.forename: Yi-Tao
authors.initials: YT
authors.lastname: Long
authors.forename: Hong-Yuan
authors.initials: HY
authors.lastname: Chen
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D019187:Cadmium Compounds / Q000737:chemistry*; D045744:Cell Line, Tumor; D045304:Cytochromes c / Q000737:chemistry* / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods*; D045663:Quantum Dots; D018036:Selenium Compounds / Q000737:chemistry*; D013440:Sulfides / Q000737:chemistry*; D013481:Superoxides / Q000032:analysis*; D017967:Zinc Compounds / Q000737:chemistry*
chemical_list: D019187:Cadmium Compounds; D005456:Fluorescent Dyes; D018036:Selenium Compounds; D013440:Sulfides; D017967:Zinc Compounds; D013481:Superoxides; D045304:Cytochromes c; C058667:cadmium selenide; C031238:zinc sulfide