title: Immunohistochemical analysis of 1844 human epithelial and haematopoietic tumours and sarcomas for IDH1R132H mutation.
abstract: OBJECTIVE
  Mutations in the isocitrate dehydrogenase 1 gene have been identified recently to play a key role in diffuse astrocytoma and oligodendroglioma as well as in acute myeloid leukaemia. In glioma, IDH1R132H is the most common mutation type, which is associated with younger patient age and longer patient survival compared to wild-type status. Sequencing analyses of carcinomas and lymphomas have detected IDH1 mutations in only a small fraction of cases. In those studies, IDH1R132H was also the most frequent mutation. The aim of the present study was to analyse a comprehensive series of human tumours for IDH1R132H mutation.
  
  
  RESULTS
  A total of 1844 formalin-fixed paraffin-embedded tumours, including carcinomas, sarcomas and haematopoietic tumours were investigated immunohistochemically using a mutation-specific antibody for IDH1(R132H). Our positive control series consisted of a collection of diffuse astrocytomas and oligodendrogliomas. No IDH1R132H mutation was found in this series.
  
  
  CONCLUSIONS
  IDH1R132H mutations occur almost exclusively in glioma and acute myeloid leukaemia.
authors.affiliation: Department of Neuropathology, Institute of Pathology, Ruprecht-Karls-Universität Heidelberg, Heidelberg, Germany.
authors.forename: Felix
authors.initials: F
authors.lastname: Sahm
authors.forename: David
authors.initials: D
authors.lastname: Capper
authors.forename: Jochen
authors.initials: J
authors.lastname: Meyer
authors.forename: Christian
authors.initials: C
authors.lastname: Hartmann
authors.forename: Esther
authors.initials: E
authors.lastname: Herpel
authors.forename: Mindaugas
authors.initials: M
authors.lastname: Andrulis
authors.forename: Gunhild
authors.initials: G
authors.lastname: Mechtersheimer
authors.forename: Iver
authors.initials: I
authors.lastname: Petersen
authors.forename: Werner
authors.initials: W
authors.lastname: Paulus
authors.forename: Andreas
authors.initials: A
authors.lastname: von Deimling
mesh_terms: D002277:Carcinoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007521:Isocitrate Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D008223:Lymphoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008297:Male; D009154:Mutation; D009369:Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D012509:Sarcoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology
chemical_list: D007521:Isocitrate Dehydrogenase; C543588:IDH1 protein, human