title: Diagnosis of Chenopodium album allergy with a cocktail of recombinant allergens as a tool for component-resolved diagnosis.
abstract: Chenopodium album pollen is one of the main sources of pollen allergy in desert and semi-desert areas and contains three identified allergens, so the aim of this study is comparison of the diagnostic potential of C. album recombinant allergens in an allergenic cocktail and C. album pollen extract. Diagnostic potential of the allergenic cocktail was investigated in 32 individuals using skin prick test and obtained results were compared with the acquired results from C. album pollen extract. Specific IgE reactivity against the pollen extract and allergenic cocktail was determined by ELISA and western blotting tests. Inhibition assays were performed for the allergenic cocktail characterization. The exact sensitization profile of all patients was identified which showed that 72, 81 and 46% of allergic patients had IgE reactivity to rChe a 1, rChe a 2 and rChe a 3, respectively. Almost all of C. album allergic patients (30/32) had specific IgE against the allergenic cocktail. In addition, there was a high correlation between IgE levels against the allergenic cocktail and IgE levels against the pollen extract. The allergenic cocktail was able to completely inhibit IgE binding to natural Che a 1, Che a 2 and Che a 3 in C. album extract. In addition, positive skin test reactions were seen in allergic patients that tested by the allergenic cocktail. The reliable results obtained from this study confirmed that the allergenic cocktail with high diagnostic potential could be replaced with natural C. album allergen extracts in skin prick test and serologic tests.
authors.affiliation: Immunobiochemistry Lab, Immunology Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. nourihr851@mums.ac.ir
authors.forename: Hamid Reza
authors.initials: HR
authors.lastname: Nouri
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Sankian
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Vahedi
authors.forename: Danial
authors.initials: D
authors.lastname: Afsharzadeh
authors.forename: Leila
authors.initials: L
authors.lastname: Rouzbeh
authors.forename: Maliheh
authors.initials: M
authors.lastname: Moghadam
authors.forename: Abdolreza
authors.initials: A
authors.lastname: Varasteh
mesh_terms: D000328:Adult; D000485:Allergens / Q000235:genetics / Q000276:immunology*; D052179:Antigens, Plant / Q000235:genetics / Q000276:immunology*; D015153:Blotting, Western; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000276:immunology*; D027463:Chenopodium album / Q000276:immunology*; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D007492:Iran; D008297:Male; D008875:Middle Aged; D011058:Pollen / Q000737:chemistry / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology*; D006255:Rhinitis, Allergic, Seasonal / Q000175:diagnosis* / Q000276:immunology; D012882:Skin Tests / Q000379:methods
chemical_list: D000485:Allergens; D052179:Antigens, Plant; D002135:Calcium-Binding Proteins; C451660:Che a 1 allergen; C528040:Che a 3 allergen, Chenopodium album; D017931:DNA Primers; D018076:DNA, Complementary; D011994:Recombinant Proteins; D007073:Immunoglobulin E