title: Priming by rhizobacterium protects tomato plants from biotrophic and necrotrophic pathogen infections through multiple defense mechanisms.
abstract: A selected strain of rhizobacterium, Pseudomonas putida strain LSW17S (LSW17S), protects tomato plants (Lycopersicon esculentum L. cv. Seokwang) from bacterial speck by biotrophic Pseudomonas syringae pv. tomato strain DC3000 (DC3000) and bacterial wilt by necrotrophic Ralstonia solanacearum KACC 10703 (Rs10703). To investigate defense mechanisms induced by LSW17S in tomato plants, transcription patterns of pathogenesis-related (PR) genes and H(2)O(2) production were analyzed in plants treated with LSW17S and subsequent pathogen inoculation. LSW17S alone did not induce transcriptions of employed PR genes in leaves and roots. DC3000 challenge following LSW17S triggered rapid transcriptions of PR genes and H(2)O(2) production in leaves and roots. Catalase infiltration with DC3000 attenuated defense-related responses and resistance against DC3000 infection. Despite depriving H(2)O(2) production and PR1b transcription by the same treatment, resistance against Rs10703 infection was not deterred significantly. H(2)O(2) is indispensable for defense signaling and/or mechanisms primed by LSW17S and inhibition of bacterial speck, however, it is not involved in resistance against bacterial wilt.
authors.affiliation: Rural Development Administration, National Academy Agricultural Science, Suwon, 441-707, Korea, jinhyung@korea.kr
authors.forename: Il-Pyung
authors.initials: IP
authors.lastname: Ahn
authors.forename: Sang-Woo
authors.initials: SW
authors.lastname: Lee
authors.forename: Min Gab
authors.initials: MG
authors.lastname: Kim
authors.forename: Sang-Ryeol
authors.initials: SR
authors.lastname: Park
authors.forename: Duk-Ju
authors.initials: DJ
authors.lastname: Hwang
authors.forename: Shin-Chul
authors.initials: SC
authors.lastname: Bae
mesh_terms: D015169:Colony Count, Microbial; D003470:Culture Media; D018506:Gene Expression Regulation, Plant; D006861:Hydrogen Peroxide / Q000378:metabolism*; D018551:Solanum lycopersicum / Q000235:genetics / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology* / Q000382:microbiology / Q000517:prevention & control; D057865:Plant Immunity; D018515:Plant Leaves / Q000235:genetics / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D016958:Pseudomonas putida / Q000502:physiology*; D044224:Pseudomonas syringae / Q000254:growth & development* / Q000472:pathogenicity; D043368:Ralstonia solanacearum / Q000254:growth & development* / Q000472:pathogenicity; D015398:Signal Transduction / Q000235:genetics; D013559:Symbiosis
chemical_list: D003470:Culture Media; D010940:Plant Proteins; D006861:Hydrogen Peroxide