title: Facile fabrication of recyclable and active nanobiocatalyst: purification and immobilization of enzyme in one pot with Ni-NTA functionalized magnetic nanoparticle.
abstract: Ni-NTA functionalized iron oxide magnetic nanoparticle was synthesized and used to selectively immobilize a his-tagged enzyme from cell free extract as an active and recyclable nanobiocatalyst, where purification and immobilization of the target enzyme were accomplished in one pot.
authors.affiliation: Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore 117576.
authors.forename: Wen
authors.initials: W
authors.lastname: Wang
authors.forename: Daniel I C
authors.initials: DI
authors.lastname: Wang
authors.forename: Zhi
authors.initials: Z
authors.lastname: Li
mesh_terms: D055162:Biocatalysis; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D004851:Epoxide Hydrolases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D008280:Magnetics; D008958:Models, Molecular; D053758:Nanoparticles / Q000737:chemistry*; D009532:Nickel / Q000737:chemistry*; D009571:Nitrilotriacetic Acid / Q000737:chemistry*; D011487:Protein Conformation; D011198:Solanum tuberosum / Q000201:enzymology
chemical_list: D004800:Enzymes, Immobilized; D009532:Nickel; D004851:Epoxide Hydrolases; D009571:Nitrilotriacetic Acid