title: [Effect of subchronic fluoride exposure on pathologic change and beta-catenin expression in rat bone tissue].
abstract: OBJECTIVE
  To explore the effect of subchronic fluoride exposure on the expression of beta-catenin in the bone of rats and the role of beta-catenin in skeletal damage.
  
  
  METHODS
  Wistar rats were randomly divided into four groups. The rats were treated at the doses of 50, 100 and 150 mg/L F- in drinking water, and control rats were drunk tap water. The contents of fluoride in bone and serum were determined after three month. The expressions of beta-catenin were analyzed by real-time RT-PCR and immunohistochemistry.
  
  
  RESULTS
  HE staining indicated that the compact bone was thicker and the trabecular bone increased in fluoride-treated group in comparison with control group. The hypertrophic chondrocytes accumulated and arranged disordered in fluoride-treated rats. The expressions of p-catenin mRNA and protein expressions in bones of fluoride-treated rats were higher than those of control group. Immunohistochemistry indicated that beta-catenins were mainly expressed by osteoblast and osteoclast. There was also positive staining in hypertrophic chondrocyte.
  
  
  CONCLUSIONS
  It was suggested that beta-catenin could play important role in bone sclerosis of subchronic fluoride exposure.
authors.affiliation: Department of Biogeochemical Disease, Liaoning Provincial Key Laboratory of Arsenic Biological Effect, China Medical University, Shenyang, China. xyguo@mail.com.cn
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Guo
authors.forename: Sisi
authors.initials: S
authors.lastname: Wu
authors.forename: Yang
authors.initials: Y
authors.lastname: He
authors.forename: Zhiyu
authors.initials: Z
authors.lastname: Zhang
authors.forename: Guifan
authors.initials: G
authors.lastname: Sun
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000378:metabolism* / Q000473:pathology*; D019902:Chondrocytes / Q000473:pathology; D004781:Environmental Exposure / Q000009:adverse effects*; D005260:Female; D005459:Fluorides / Q000633:toxicity*; D006984:Hypertrophy; D008297:Male; D010026:Osteosclerosis / Q000139:chemically induced / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism*
chemical_list: C495268:Ctnnb1 protein, rat; D012333:RNA, Messenger; D051176:beta Catenin; D005459:Fluorides