title: A novel strategy to derive iPS cells from porcine fibroblasts.
abstract: Induced pluripotent stem (iPS) cell technology demonstrates that somatic cells can be reprogrammed to a pluripotent state by over-expressing four reprogramming factors. This technology has created an interest in deriving iPS cells from domesticated animals such as pigs, sheep and cattle. Moloney murine leukemia retrovirus vectors have been widely used to generate and study mouse iPS cells. However, this retrovirus system infects only mouse and rat cells, which limits its use in establishing iPS cells from other mammals. In our study, we demonstrate a novel retrovirus strategy to efficiently generate porcine iPS cells from embryonic fibroblasts. We transfected four human reprogramming factors (Oct4, Sox2, Klf4 and Myc) into fibroblasts in one step by using a VSV-G envelope-coated pantropic retrovirus that was easily packaged by GP2-293 cells. We established six embryonic stem (ES)-like cell lines in human ES cell medium supplemented with bFGF. Colonies showed a similar morphology to human ES cells with a high nuclei-cytoplasm ratio and phase-bright flat colonies. Porcine iPS cells could form embryoid bodies in vitro and differentiate into the three germ layers in vivo by forming teratomas in immunodeficient mice.
authors.affiliation: State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China.
authors.forename: WeiMin
authors.initials: W
authors.lastname: Ruan
authors.forename: JianYong
authors.initials: J
authors.lastname: Han
authors.forename: Pin
authors.initials: P
authors.lastname: Li
authors.forename: SuYing
authors.initials: S
authors.lastname: Cao
authors.forename: Yang
authors.initials: Y
authors.lastname: An
authors.forename: Bing
authors.initials: B
authors.lastname: Lim
authors.forename: Ning
authors.initials: N
authors.lastname: Li
mesh_terms: D000818:Animals; D002417:Cattle; D054337:Cell Dedifferentiation / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology*; D002460:Cell Line; D058732:Embryoid Bodies / Q000166:cytology / Q000502:physiology; D005347:Fibroblasts / Q000502:physiology*; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000502:physiology*; D007621:Karyotyping; D000090062:Kruppel-Like Factor 4; D051379:Mice; D016513:Mice, SCID; D051381:Rats; D012190:Retroviridae / Q000502:physiology; D013552:Swine; D013724:Teratoma / Q000378:metabolism / Q000473:pathology