title: Adjuvant effects of salidroside from Rhodiola rosea L. on the immune responses to ovalbumin in mice.
abstract: Salidroside, a major component of Rhodiola rosea L., was evaluated for its adjuvant effects on the immune responses in mice by ovalbumin (OVA) stimulation. BALB/c mice were immunized subcutaneously with OVA 100 μg or OVA 100 μg dissolved in saline containing alum (100 μg) or salidroside (12.5, 25, or 50 μg) on Days 1 and 15. Two weeks later (Day 28), blood samples were collected to analyze OVA-specific IgG, IgG1, and IgG2b antibodies. Meanwhile, splenocytes were harvested to assess lymphocyte proliferation, cytokines (IL-2, IL-4, and IFN-γ) production, and CD4(+), CD8(+) lymphocyte subsets. The results indicated that co-administration of salidroside with OVA significantly enhanced the ConA-, LPS-, and OVA-induced splenocyte proliferation, produced more IL-2, IL-4, IFN-γ, and IgG, IgG1, and IgG2b antibody levels, and increased the percentage of CD4(+), CD8(+) lymphocyte subsets than OVA alone. Thus, salidroside possess immunological adjuvant activity by regulating humoral and cellular immune responses in mice.
authors.affiliation: Department of Veterinary Pharmacology, College of Animal Science and Veterinary Medicine, Jilin University, Jilin, PR China. gshuang1973@126.com
authors.forename: Shuang
authors.initials: S
authors.lastname: Guan
authors.forename: Jiakang
authors.initials: J
authors.lastname: He
authors.forename: Weixiao
authors.initials: W
authors.lastname: Guo
authors.forename: Jingyuan
authors.initials: J
authors.lastname: Wei
authors.forename: Jing
authors.initials: J
authors.lastname: Lu
authors.forename: Xuming
authors.initials: X
authors.lastname: Deng
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D016207:Cytokines / Q000096:biosynthesis / Q000276:immunology; D005960:Glucosides / Q000737:chemistry / Q000494:pharmacology*; D007111:Immunity, Cellular / Q000187:drug effects* / Q000276:immunology; D056724:Immunity, Humoral / Q000187:drug effects* / Q000276:immunology; D007114:Immunization / Q000379:methods; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology / Q000494:pharmacology*; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology*; D029771:Rhodiola / Q000737:chemistry*
chemical_list: D000276:Adjuvants, Immunologic; D016207:Cytokines; D005960:Glucosides; D007074:Immunoglobulin G; D010636:Phenols; D010047:Ovalbumin; C009172:rhodioloside