title: Favorable response of chronic refractory immune thrombocytopenic purpura to mesenchymal stem cells.
abstract: Seven patients with chronic refractory immune thrombocytopenic purpura (ITP) received adipose tissue-derived mesenchymal stem cells (AMSC) from haplo-identical family donors. The AMSC dose was 2.0×10(6)/kg. No side effects were noted after the AMSC infusions. Overall responses were reached in all patients and sustained response rate was 57.1% (4/7). The serum levels of transforming growth factor β1 (TGF-β1), interleukin (IL)-4, and IL-10 were significantly elevated, whereas those of interferon-γ (IFN-γ) and IL-2 were significantly decreased after AMSC administration, compared with those in the patients with active ITP. During follow-up, the cytokine profiles in patients maintaining sustained response remained stable compared with the post-treatment level, but IFN-γ and IL-2 levels were significantly increased, and those of TGF-β1, IL-4, and IL-10 were significantly reduced again in relapsed patients. AMSC therapy seems to represent reasonable salvage treatment in severe, chronic refractory ITP by causing a shift in the Th1/Th2 cytokine balance to the same levels as normal controls.
authors.affiliation: Henan Key Laboratory of Experimental Haematology, Henan Institute of Haematology, Henan Tumor Hospital, Zhengzhou University, Zhengzhou, China.
authors.forename: Baijun
authors.initials: B
authors.lastname: Fang
authors.forename: Ling
authors.initials: L
authors.lastname: Mai
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.forename: Yongping
authors.initials: Y
authors.lastname: Song
mesh_terms: D000328:Adult; D002908:Chronic Disease / Q000628:therapy; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007371:Interferon-gamma / Q000097:blood; D016753:Interleukin-10 / Q000097:blood; D007376:Interleukin-2 / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D008875:Middle Aged; D010976:Platelet Count; D011696:Purpura, Thrombocytopenic / Q000097:blood / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D050507:Subcutaneous Fat, Abdominal / Q000166:cytology / Q000378:metabolism / Q000637:transplantation; D053773:Transforming Growth Factor beta1 / Q000097:blood; D055815:Young Adult
chemical_list: C508609:IL10 protein, human; C508594:IL2 protein, human; C508598:IL4 protein, human; D007376:Interleukin-2; D053773:Transforming Growth Factor beta1; D016753:Interleukin-10; D015847:Interleukin-4; D007371:Interferon-gamma