title: Morphological features of osteoblasts cultured on ultraviolet-irradiated titanium plates.
abstract: BACKGROUND
  Although we have recently established optimal experimental conditions of ultraviolet (UV)-irradiation for titanium plates (e.g. wavelength and exposure time) which enhanced osteoblast adhesion to the plates, the effects of UV-irradiation on cell structure are still unclear.
  
  
  METHODS
  Digital stereomicroscopy was used to investigate morphological alterations of non-stained viable and hematoxylin-eosin (HE)-stained cells on UV-irradiated and non-UV-irradiated titanium plates for up to 24 hours.
  
  
  RESULTS
  In 24 hours, significant expansion of HE-stained cells (area, perimeter and sprouting processes) was observed on UV-irradiated plates. The sprouting processes appeared within 40 minutes of inoculation under both conditions, however, significant cell area expansion, which occurred in 5 minutes, was observed only on UV-irradiated plates.
  
  
  CONCLUSIONS
  UV-enhanced cell attachment was related to morphological alteration which occurred immediately after inoculation. Digital stereomicroscopic evaluation was able to define and quantify morphological alterations of viable cells in an opaque environment.
authors.affiliation: Dental Research Center (CIEAO), Faculty of Dentistry, Autonomous University of State of Mexico (UAEM), Toluca, Estado de Mexico, Mexico.
authors.forename: René
authors.initials: R
authors.lastname: García-Contreras
authors.forename: Shuichiro
authors.initials: S
authors.lastname: Kanagawa
authors.forename: Yuji
authors.initials: Y
authors.lastname: Beppu
authors.forename: Takahide
authors.initials: T
authors.lastname: Nagao
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sakagami
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nakajima
authors.forename: Jun
authors.initials: J
authors.lastname: Shimada
authors.forename: Kazunori
authors.initials: K
authors.lastname: Adachi
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000295:instrumentation; D002460:Cell Line; D002478:Cells, Cultured; D051379:Mice; D008810:Mice, Inbred C57BL; D010006:Osteoblasts / Q000166:cytology* / Q000528:radiation effects; D013499:Surface Properties / Q000528:radiation effects; D014025:Titanium; D014466:Ultraviolet Rays
chemical_list: D014025:Titanium