title: Impaired deoxyribonuclease I activity in patients with inflammatory bowel diseases.
abstract: Background and Aims. Deoxyribonuclease I (DNaseI) is an endonuclease that facilitates chromatin breakdown and promotes susceptibility to autoimmune disorders. The aim of current study was to investigate serum DNase I activity in patients with inflammatory bowel diseases (IBD). Patients and Methods. A cohort of 110 IBD patients was evaluated, aged 35 ± 12 years, 77 with Crohn's disease (CD) and 33 with ulcerative colitis (UC). 50 SLE patients and 50 healthy blood donors were examined as control groups. Results. DNase I activity in IBD patients was significantly lower than in healthy individuals, but higher than in SLE patients (P < .0001). Patients with UC showed higher DNase I activity than CD patients, P = .21. DNase I activity in female patients with IBD was significantly lower than in males, P = .024; however, no differences in DNase I activity were found in relation to gender in healthy individuals. DNase I activity has shown a strong negative correlation with the serum concentration of anti-nucleosomal antibodies in the autoimmune (SLE + IBD) cohort, as well as in the separate IBD cohort. Conclusions. Reduced serum DNase I activity probably has pathogenetic consequences in IBD. Induction of autoantibodies towards nucleosomes could be a reflection of impaired DNase I activity.
authors.affiliation: Institute of Clinical Biochemistry and Laboratory Diagnostics, General University Hospital in Prague, First Faculty of Medicine, Charles University in Prague, Karlovo nam. 32, 121 11 Prague 2, Czech Republic.
authors.forename: Karin
authors.initials: K
authors.lastname: Malíčková
authors.forename: Dana
authors.initials: D
authors.lastname: Duricová
authors.forename: Martin
authors.initials: M
authors.lastname: Bortlík
authors.forename: Zdenka
authors.initials: Z
authors.lastname: Hrušková
authors.forename: Barbora
authors.initials: B
authors.lastname: Svobodová
authors.forename: Naděžda
authors.initials: N
authors.lastname: Machková
authors.forename: Viktor
authors.initials: V
authors.lastname: Komárek
authors.forename: Terezie
authors.initials: T
authors.lastname: Fučíková
authors.forename: Ivana
authors.initials: I
authors.lastname: Janatková
authors.forename: Tomáš
authors.initials: T
authors.lastname: Zima
authors.forename: Milan
authors.initials: M
authors.lastname: Lukáš