title: Drug intercalation in layered double hydroxide clay: application in the development of a nanocomposite film for guided tissue regeneration.
abstract: It has been proposed that localized and controlled delivery of alendronate and tetracycline to periodontal pocket fluids via guided tissue regeneration (GTR) membranes may be a valuable adjunctive treatment for advanced periodontitis. The objectives of this work were to develop a co-loaded, controlled release tetracycline and alendronate nanocomposite plasticized poly(lactic-co-glycolic acid) (PLGA) film that would form a suitable matrix supporting osteoblast proliferation and differentiation. Alendronate release was successfully controlled, with complete suppression of the burst phase of release by intercalation of alendronate anions in magnesium/aluminum layered double hydroxide (LDH) clay nanoparticles and dispersed in the PLGA film matrix. Tetracycline, loaded as free drug into the film together with alendronate-LDH clay complex released more rapidly than alendronate, but showed evidence of intercalation in the LDH clay particles. The dual drug loaded nanocomposite films were biocompatible with osteoblasts and after 5 week incubations, significant increase in alkaline phosphatase activity and bone nodule formation were observed.
authors.affiliation: University of British Columbia, Vancouver, BC, Canada.
authors.forename: Michelle
authors.initials: M
authors.lastname: Chakraborti
authors.forename: John K
authors.initials: JK
authors.lastname: Jackson
authors.forename: David
authors.initials: D
authors.lastname: Plackett
authors.forename: Donald M
authors.initials: DM
authors.lastname: Brunette
authors.forename: Helen M
authors.initials: HM
authors.lastname: Burt
mesh_terms: D019386:Alendronate / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics*; D000469:Alkaline Phosphatase / Q000378:metabolism; D000538:Aluminum Silicates / Q000737:chemistry / Q000493:pharmacokinetics; D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D000077215:Clay; D004337:Drug Carriers / Q000737:chemistry / Q000493:pharmacokinetics; D004338:Drug Combinations; D048091:Guided Tissue Regeneration / Q000379:methods*; D006878:Hydroxides / Q000737:chemistry; D019344:Lactic Acid / Q000737:chemistry* / Q000493:pharmacokinetics; D053761:Nanocomposites / Q000008:administration & dosage / Q000737:chemistry*; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects*; D010316:Particle Size; D011100:Polyglycolic Acid / Q000737:chemistry* / Q000493:pharmacokinetics; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D051381:Rats; D017207:Rats, Sprague-Dawley; D013499:Surface Properties; D013752:Tetracycline / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics
chemical_list: D000538:Aluminum Silicates; D001672:Biocompatible Materials; D004337:Drug Carriers; D004338:Drug Combinations; D006878:Hydroxides; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D000469:Alkaline Phosphatase; D013752:Tetracycline; D000077215:Clay; D019386:Alendronate