title: Design and integration of a generic disposable array-compatible sensor housing into an integrated disposable indirect microfluidic flow injection analysis system.
abstract: We describe an integration strategy for arbitrary sensors intended to be used as biosensors in biomedical or bioanalytical applications. For such devices ease of handling (by a potential end user) as well as strict disposable usage are of importance. Firstly we describe a generic array compatible polymer sensor housing with an effective sample volume of 1.55 μl. This housing leaves the sensitive surface of the sensor accessible for the application of biosensing layers even after the embedding. In a second step we show how this sensor housing can be used in combination with a passive disposable microfluidic chip to set up arbitrary 8-fold sensor arrays and how such a system can be complemented with an indirect microfluidic flow injection analysis (FIA) system. This system is designed in a way that it strictly separates between disposable and reusable components- by introducing tetradecane as an intermediate liquid. This results in a sensor system compatible with the demands of most biomedical applications. Comparative measurements between a classical macroscopic FIA system and this integrated indirect microfluidic system are presented. We use a surface acoustic wave (SAW) sensor as an exemplary detector in this work.
authors.affiliation: Institute of Microstructure Technology, Karlsruhe Institute of Technology, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany. Bastian.Rapp@kit.edu
authors.forename: Bastian E
authors.initials: BE
authors.lastname: Rapp
authors.forename: Benjamin
authors.initials: B
authors.lastname: Schickling
authors.forename: Jürgen
authors.initials: J
authors.lastname: Prokop
authors.forename: Volker
authors.initials: V
authors.lastname: Piotter
authors.forename: Michael
authors.initials: M
authors.lastname: Rapp
authors.forename: Kerstin
authors.initials: K
authors.lastname: Länge
mesh_terms: D000042:Absorption; D015374:Biosensing Techniques / Q000295:instrumentation; D004867:Equipment Design; D017022:Flow Injection Analysis / Q000295:instrumentation; D056656:Lab-On-A-Chip Devices; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D011506:Proteins / Q000032:analysis / Q000737:chemistry
chemical_list: D011506:Proteins