title: Nerve growth factor induced expression of iNOS and substance P in dorsal root ganglion sensory neuron and interferon regulatory factor-1.
abstract: OBJECTIVE
  To investigate the mechanism of airway neurogenic inflammation by studying the expression of inducible nitric oxide synthase (iNOS) and substance P in C(7)-T(5) dorsal root ganglion sensory neuron cells induced by nerve growth factor (NGF) and the role of interferon regulatory factor-1 (IRF-1).
  
  
  METHODS
  The dorsal root ganglia neuron (DRGn) cells were primary cultured, and then stimulated with or without NGF or NGF+interferon (IFN)-γ. Subsequently the DRGn cells were transinfected with or without green fluorescent protein (GFP)-IRF-1-vshRNA, and then stimulated with or without NGF. The expressions of iNOS and substance P were detected by real-time PCR.
  
  
  RESULTS
  NGF induced the mRNA expression of iNOS and substance P in dorsal root ganglion sensory neuron cells, and IFN-γ increased NGF-induced iNOS mRNA expression. The expressions of iNOS and substance P in sensory neuron cells were decreased significantly at the mRNA level after IRF-1 was blocked down by IRF-1-vshRNA transinfection.
  
  
  CONCLUSIONS
  NGF is involved in the airway neurogenic inflammation by prompting the expression of iNOS and substance P through transcription factor IRF-1 in airway sensory neuron cells. IRF-1 may be a therapeutic target for airway neurogenic inflammation.
authors.affiliation: Department of Respiratory Medicine, Xiangya Hospital, Central South University, Changsha 410008, China. pinhuapan668@hotmail.com
authors.forename: Pinhua
authors.initials: P
authors.lastname: Pan
authors.forename: Siyun
authors.initials: S
authors.lastname: Huang
authors.forename: Chengping
authors.initials: C
authors.lastname: Hu
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D005260:Female; D005727:Ganglia, Spinal / Q000166:cytology / Q000378:metabolism*; D007249:Inflammation; D050856:Interferon Regulatory Factor-1 / Q000378:metabolism*; D020932:Nerve Growth Factor / Q000494:pharmacology*; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism*; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012137:Respiratory System / Q000166:cytology; D011984:Sensory Receptor Cells / Q000378:metabolism; D013373:Substance P / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
chemical_list: D050856:Interferon Regulatory Factor-1; D012333:RNA, Messenger; D013373:Substance P; D020932:Nerve Growth Factor; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat