title: A novel monoclonal antibody specific to mouse IL-17.
abstract: IL-17 is secreted by T helper (Th) 17 cells, and might play important roles in host defense against extracellular bacterial infection and fungal infection and contribute to the pathogenesis of various autoimmune inflammatory diseases. Here we have, for the first time, generated the monoclonal antibody against the rat IL-17 protein, which is secreted by T helper (Th) 17 cells. Enzyme-linked immunosorbent assay (ELISA) and Western immunoblotting were used to screen the hybridomas. As a result, one MAb named Y11 (IgG1) was characterized, which was effective in detecting the recombinant and the cellular protein. Then the MAb was used to detect the expression of IL-17 in rat intestine graft tissues by immunohistochemical analysis. Thus, Y11 could be considered a useful tool for studies of IL-17 expression and function.
authors.affiliation: Department of Digestive Surgery, Xijing Hospital of Digestive Diseases, Fourth Military Medical University of China, Xi'an, Shaanxi Province, China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Yang
authors.forename: Liu
authors.initials: L
authors.lastname: Hong
authors.forename: Weizhong
authors.initials: W
authors.lastname: Wang
authors.forename: Hongwei
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D006825:Hybridomas / Q000276:immunology*; D007150:Immunohistochemistry; D020381:Interleukin-17 / Q000276:immunology*; D051379:Mice; D051381:Rats; D058504:Th17 Cells / Q000276:immunology / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D020381:Interleukin-17