title: TimeLapseAnalyzer: multi-target analysis for live-cell imaging and time-lapse microscopy.
abstract: The direct observation of cells over time using time-lapse microscopy can provide deep insights into many important biological processes. Reliable analyses of motility, proliferation, invasive potential or mortality of cells are essential to many studies involving live cell imaging and can aid in biomarker discovery and diagnostic decisions. Given the vast amount of image- and time-series data produced by modern microscopes, automated analysis is a key feature to capitalize the potential of time-lapse imaging devices. To provide fast and reproducible analyses of multiple aspects of cell behaviour, we developed TimeLapseAnalyzer. Apart from general purpose image enhancements and segmentation procedures, this extensible, self-contained, modular cross-platform package provides dedicated modalities for fast and reliable analysis of multi-target cell tracking, scratch wound healing analysis, cell counting and tube formation analysis in high throughput screening of live-cell experiments. TimeLapseAnalyzer is freely available (MATLAB, Open Source) at http://www.informatik.uni-ulm.de/ni/mitarbeiter/HKestler/tla.
authors.affiliation: Department of Gastroenterology and Endocrinology, University Hospital of Marburg, Germany.
authors.forename: Johannes
authors.initials: J
authors.lastname: Huth
authors.forename: Malte
authors.initials: M
authors.lastname: Buchholz
authors.forename: Johann M
authors.initials: JM
authors.lastname: Kraus
authors.forename: Kristian
authors.initials: K
authors.lastname: Mølhave
authors.forename: Cristian
authors.initials: C
authors.lastname: Gradinaru
authors.forename: Götz
authors.initials: G
authors.lastname: v Wichert
authors.forename: Thomas M
authors.initials: TM
authors.lastname: Gress
authors.forename: Heiko
authors.initials: H
authors.lastname: Neumann
authors.forename: Hans A
authors.initials: HA
authors.lastname: Kestler
mesh_terms: D008853:Microscopy / Q000379:methods*; D015203:Reproducibility of Results