title: Magnetic resonance imaging quantification of regional cerebral blood flow and cerebrovascular reactivity to carbon dioxide in normotensive and hypertensive rats.
abstract: Hypertension afflicts 25% of the general population and over 50% of the elderly. In the present work, arterial spin labeling MRI was used to non-invasively quantify regional cerebral blood flow (CBF), cerebrovascular resistance and CO(2) reactivity in spontaneously hypertensive rats (SHR) and in normotensive Wistar Kyoto rats (WKY), at two different ages (3 months and 10 months) and under the effects of two anesthetics, α-chloralose and 2% isoflurane (1.5 MAC). Repeated CBF measurements were highly consistent, differing by less than 10% and 18% within and across animals, respectively. Under α-chloralose, whole brain CBF at normocapnia did not differ between groups (young WKY: 61 ± 3ml/100g/min; adult WKY: 62 ± 4ml/100g/min; young SHR: 70 ± 9ml/100g/min; adult SHR: 69 ± 8ml/100g/min), indicating normal cerebral autoregulation in SHR. At hypercapnia, CBF values increased significantly, and a linear relationship between CBF and PaCO(2) levels was observed. In contrast, 2% isoflurane impaired cerebral autoregulation. Whole brain CBF in SHR was significantly higher than in WKY rats at normocapnia (young SHR: 139 ± 25ml/100g/min; adult SHR: 104 ± 23ml/100g/min; young WKY: 55± 9ml/100g/min; adult WKY: 71 ± 19ml/100g/min). CBF values increased significantly with increasing CO(2); however, there was a clear saturation of CBF at PaCO(2) levels greater than 70mmHg in both young and adult rats, regardless of absolute CBF values, suggesting that isoflurane interferes with the vasodilatory mechanisms of CO(2). This behavior was observed for both cortical and subcortical structures. Under either anesthetic, CO(2) reactivity values in adult SHR were decreased, confirming that hypertension, when combined with age, increases cerebrovascular resistance and reduces cerebrovascular compliance.
authors.affiliation: National Institute of Neurological Disorders and Stroke, NIH, Bethesda, MD 20892-1065, USA.
authors.forename: Renata F
authors.initials: RF
authors.lastname: Leoni
authors.forename: Fernando F
authors.initials: FF
authors.lastname: Paiva
authors.forename: Erica C
authors.initials: EC
authors.lastname: Henning
authors.forename: George C
authors.initials: GC
authors.lastname: Nascimento
authors.forename: Alberto
authors.initials: A
authors.lastname: Tannús
authors.forename: Draulio B
authors.initials: DB
authors.lastname: de Araujo
authors.forename: Afonso C
authors.initials: AC
authors.lastname: Silva
mesh_terms: D000375:Aging / Q000502:physiology; D000758:Anesthesia; D018685:Anesthetics, Inhalation / Q000494:pharmacology; D018686:Anesthetics, Intravenous / Q000494:pharmacology; D000818:Animals; D002245:Carbon Dioxide / Q000097:blood / Q000494:pharmacology*; D002560:Cerebrovascular Circulation / Q000187:drug effects*; D002698:Chloralose / Q000494:pharmacology; D006706:Homeostasis / Q000187:drug effects / Q000502:physiology; D006973:Hypertension / Q000503:physiopathology*; D007530:Isoflurane / Q000494:pharmacology; D008279:Magnetic Resonance Imaging; D008297:Male; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D013113:Spin Labels; D014655:Vascular Resistance / Q000187:drug effects / Q000502:physiology
chemical_list: D018685:Anesthetics, Inhalation; D018686:Anesthetics, Intravenous; D013113:Spin Labels; D002245:Carbon Dioxide; D002698:Chloralose; D007530:Isoflurane