title: Surface plasmon resonance spectroscopy: a new lead in studying the membrane binding of amyloidogenic transthyretin.
abstract: Surface plasmon resonance (SPR) employs the optical principle of SPR to measure changes in mass on a sensor chip surface in real time. Surface chemistry has been developed which enables the immoblization of lipid bilayers and determination of protein-membrane interactions in real time. In the last decade, the plasma membrane has been demonstrated to play an important role in amyloidogenesis and cytotoxicity induced by amyloidogenic proteins. SPR provides an ideal way to study the membrane binding of amyloidogenic proteins. In this chapter, we describe the application of SPR to the study of amyloidogenic transthyretin binding to the plasma membrane and artificial lipid bilayers.
authors.affiliation: Department of Biochemistry and Molecular Biology, Monash University, Clayton, VIC, Australia.
authors.forename: Xu
authors.initials: X
authors.lastname: Hou
authors.forename: David H
authors.initials: DH
authors.lastname: Small
authors.forename: Marie-Isabel
authors.initials: MI
authors.lastname: Aguilar
mesh_terms: D000682:Amyloid / Q000378:metabolism*; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008563:Membrane Lipids / Q000302:isolation & purification; D008565:Membrane Proteins / Q000302:isolation & purification; D011228:Prealbumin / Q000378:metabolism*; D011485:Protein Binding; D013057:Spectrum Analysis / Q000295:instrumentation / Q000379:methods*; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods*; D053835:Unilamellar Liposomes / Q000737:chemistry
chemical_list: D000682:Amyloid; D008563:Membrane Lipids; D008565:Membrane Proteins; D011228:Prealbumin; D053835:Unilamellar Liposomes