title: Transcript profiling of the meiotic drive phenotype in testis of Aedes aegypti using suppressive subtractive hybridization.
abstract: The meiotic drive gene in Aedes aegypti is tightly linked with the sex determination locus on chromosome 1, and causes highly male-biased sex ratios. We prepared cDNA libraries from testes from the Ae. aegypti T37 strain (driving) and RED strain (non-driving), and used suppressive subtraction hybridization techniques to enrich for T37 testes-specific transcripts. Expressed sequence tags (ESTs) were obtained from a total of 2784 randomly selected clones from the subtracted T37 (subT37) library as well as the primary libraries for each strain (pT37 and pRED). Sequence analysis identified a total of 171 unique genes in the subT37 library and 299 unique genes among the three libraries. The majority of genes enriched in the subT37 library were associated with signal transduction, development, reproduction, metabolic process and cell cycle functions. Further, as observed with meiotic drive systems in Drosophila and mouse, a number of these genes were associated with signaling cascades that involve the Ras superfamily of regulatory small GTPases. Differential expression of several of these genes was verified in Ae. aegypti pupal testes using qRT-PCR. This study increases our understanding of testes gene expression enriched in adult males from the meiotic drive strain as well as insights into the basic testes transcriptome in Ae. aegypti.
authors.affiliation: Eck Institute for Global Health, Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556-5645, USA. dshin@nd.edu
authors.forename: Dongyoung
authors.initials: D
authors.lastname: Shin
authors.forename: Lizhong
authors.initials: L
authors.lastname: Jin
authors.forename: Neil F
authors.initials: NF
authors.lastname: Lobo
authors.forename: David W
authors.initials: DW
authors.lastname: Severson
mesh_terms: D000330:Aedes / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D020224:Expressed Sequence Tags; D005260:Female; D020869:Gene Expression Profiling; D015723:Gene Library; D008297:Male; D008540:Meiosis; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D019849:Sex Determination Processes; D012744:Sex Ratio; D013737:Testis / Q000378:metabolism