title: Establishment of a novel ovine kidney cell line for isolation and propagation of viruses infecting domestic cloven-hoofed animal species.
abstract: A sheep kidney-derived cell line, FLK-N3, was successfully established after serial (>100) passages. Persistent infection of this cell line with viruses and mycoplasma was not detected. The cells grew well and showed susceptibility to a wide variety of viruses derived from ovine, bovine, and porcine species, including orf virus, maedi visna virus, bovine herpesvirus 1, bovine parainfluenza virus 3, bovine viral diarrhea viruses 1 and 2, bovine coronavirus, bovine respiratory syncytial virus, bovine enterovirus, suid herpesvirus 1, and porcine enterovirus. These results suggest that the FLK-N3 cell line could be useful for isolation and propagation of viruses that affect cloven-hoofed animals.
authors.affiliation: National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba, Ibaraki, Japan.
authors.forename: Katsuyoshi
authors.initials: K
authors.lastname: Matsuura
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Inoshima
authors.forename: Ken-ichiro
authors.initials: K
authors.lastname: Kameyama
authors.forename: Kenji
authors.initials: K
authors.lastname: Murakami
mesh_terms: D000818:Animals; D000829:Animals, Domestic / Q000821:virology*; D002417:Cattle / Q000821:virology; D002460:Cell Line; D006041:Goats / Q000821:virology; D007668:Kidney / Q000166:cytology*; D012756:Sheep / Q000033:anatomy & histology* / Q000821:virology; D013552:Swine / Q000821:virology; D014777:Virus Diseases / Q000662:veterinary*; D014779:Virus Replication; D014780:Viruses / Q000472:pathogenicity*