title: In vitro dual culture of Polymyxa betae in Agrobacterium rhizogenes transformed sugar beet hairy roots in liquid media.
abstract: Polymyxa betae is a soil-borne protist and an obligate parasite of sugar beet that transmits the beet necrotic yellow vein virus. Sugar beet hairy roots, transformed by Agrobacterium rhizogenes, were inoculated with surface-sterilized root fragments infected by P. betae. After 10 wk in a liquid medium, typical structures of P. betae were observed in this in vitro system. This first in vitro culture of P. betae in liquid medium will contribute to a better understanding of this protist's biology through providing a way to conserve and produce purified isolates of the protist.
authors.affiliation: Université Catholique de Louvain, Earth and Life Institute, Applied Microbiology, Phytopathology, Croix du Sud L7.05.03 B-1348 Louvain-la-neuve, Belgium.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Desoignies
authors.forename: Anne
authors.initials: A
authors.lastname: Legreve
mesh_terms: D060054:Agrobacterium / Q000235:genetics / Q000502:physiology; D027461:Beta vulgaris / Q000166:cytology / Q000235:genetics* / Q000382:microbiology / Q000469:parasitology*; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D010935:Plant Diseases / Q000469:parasitology*; D018517:Plant Roots / Q000166:cytology / Q000235:genetics / Q000382:microbiology / Q000469:parasitology; D056909:Plasmodiophorida / Q000254:growth & development*; D014170:Transformation, Genetic