title: Biosynthesis of glycosylated derivatives of tylosin in Streptomyces venezuelae.
abstract: Streptomyces venezuelae YJ028, bearing a deletion of the entire biosynthetic gene cluster encoding the pikromycin polyketide synthases and desosamine biosynthetic enzymes, was used as a bioconversion system for combinatorial biosynthesis of glycosylated derivatives of tylosin. Two engineered deoxysugar biosynthetic pathways for the biosynthesis of TDP-3-O-demethyl-D-chalcose or TDP-Lrhamnose in conjunction with the glycosyltransferaseauxiliary protein pair DesVII/DesVIII were expressed in a S. venezuelae YJ028 mutant strain. Supplementation of each mutant strain capable of producing TDP-3-O-demethyl- D-chalcose or TDP-L-rhamnose with tylosin aglycone tylactone resulted in the production of the 3-O-demethyl- D-chalcose, D-quinovose, or L-rhamnose-glycosylated tylactone.
authors.affiliation: Interdisciplinary Programs of Bioengineering, Seoul National University, Seoul, Korea.
authors.forename: Ah Reum
authors.initials: AR
authors.lastname: Han
authors.forename: Sung Ryeol
authors.initials: SR
authors.lastname: Park
authors.forename: Je Won
authors.initials: JW
authors.lastname: Park
authors.forename: Eun Yeol
authors.initials: EY
authors.lastname: Lee
authors.forename: Dong-Myung
authors.initials: DM
authors.lastname: Kim
authors.forename: Byung-Gee
authors.initials: BG
authors.lastname: Kim
authors.forename: Yeo Joon
authors.initials: YJ
authors.lastname: Yoon
mesh_terms: D000606:Amino Sugars / Q000096:biosynthesis; D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D053898:Biosynthetic Pathways; D001709:Biotechnology / Q000379:methods; D020650:Combinatorial Chemistry Techniques; D017353:Gene Deletion; D005818:Genetic Engineering / Q000379:methods; D006031:Glycosylation; D018942:Macrolides / Q000378:metabolism; D005810:Multigene Family; D048630:Polyketide Synthases / Q000096:biosynthesis / Q000235:genetics; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*; D015645:Tylosin / Q000096:biosynthesis* / Q000737:chemistry
chemical_list: D000606:Amino Sugars; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D018942:Macrolides; C110125:desosamine; D048630:Polyketide Synthases; C003145:picromycin; D015645:Tylosin