title: [Effects of anticoagulants and storage temperature on immature platelet fraction % (IPF%) values in stored samples measured by the automated hematology analyzer, XE-5000--utility of CTAD-anticoagulation and room temperature storage].
abstract: Measurement of reticulated platelet percentage (RP%) is thought to be a useful marker for differential diagnosis and analysis of platelet kinetics in patients with thrombocytopenic disorders. Two methods are used to detect RP; flow cytometric method and immature platelet fraction (IPF) method using automated hematology analyzers. Although IPF% measured by the automated hematology analyzers is simple and convenient, we already reported that IPF% values were highly fluctuated in stored whole blood sample with EDTA-2K at 4 degrees C day by day. In this study we investigated the stability of IPF% in blood samples obtained from 11 patients with chronic immune thrombocytopenic purpura (ITP) and 19 healthy volunteers using the automated hematology analyzer, XE-5000 (Sysmex) under various storage conditions. EDTA-2K, 3.13% sodium citrate, acid-citrate dextrose solution (ACD), citrate-theophylline-adenosine-dipyridamole solution (CTAD), or sodium fluoride was used as an anticoagulant. When blood samples obtained from healthy subjects were stored at 4 degrees C, IPF% values markedly increased in a time-dependent manner by any anticoagulant examined. On the other hand, there was no significant or only slight difference in IPF% values at room temperature (RT) storage except sodium fluoride. However, in patients with ITP the elevated IPF% values fluctuated widely in EDTA-2K, sodium citrate and ACD-anticoagulated samples even at RT storage. In contrast, IPF% values in CTAD samples stored at RT were highly stable in all patients with ITP up to 4 day storage. These results suggest that the measurement of IPF% by XE-5000 provides quite stable data up to 4 day-storage in ITP patients as well as healthy subjects under CTAD-anticoagulation and RT storage conditions.
authors.affiliation: Laboratory for Clinical Investigation, Osaka University Hospital, Suita, Japan. marco@hp-lab.med.osaka-u.ac.jp
authors.forename: Miho
authors.initials: M
authors.lastname: Nishiyama
authors.forename: Satoru
authors.initials: S
authors.lastname: Hayashi
authors.forename: Osamu
authors.initials: O
authors.lastname: Kabutomori
authors.forename: Hachirou
authors.initials: H
authors.lastname: Yamanishi
authors.forename: Etsuji
authors.initials: E
authors.lastname: Suehisa
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Kurata
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Kashiwagi
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Tomiyama
mesh_terms: D000241:Adenosine; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000925:Anticoagulants; D001793:Blood Preservation / Q000379:methods*; D019343:Citric Acid; D004176:Dipyridamole; D005260:Female; D006403:Hematologic Tests / Q000295:instrumentation*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010976:Platelet Count; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000097:blood*; D012969:Sodium Fluoride; D012996:Solutions; D013696:Temperature; D013806:Theophylline
chemical_list: D000925:Anticoagulants; D012996:Solutions; D019343:Citric Acid; D004176:Dipyridamole; D012969:Sodium Fluoride; D013806:Theophylline; D000241:Adenosine