title: Viral diversity of Junín virus field strains.
abstract: The Argentine Hemorrhagic Fever, an endemic disease present in a much of Argentina, is caused by the Junín virus (JUNV). Currently, there are sequences available from several strains of this virus, like those belonging to the vaccine lineage (XJ13, XJ#44 and Candid#1), as well as MC2 (rodent isolate) and IV4454 (human isolate). In this article, we report sequence information on two fragments of genomic segment S of viral isolates from the endemic area. A Nested-RT-PCR was used to amplify discrete genomic regions of 13 isolates of rodent and human origin. The bioinformatics studies revealed a great homogeneity of sequences among the JUNV isolates. The phylogenetic classification showed greater evolutionary distance between the old world arenaviruses (Lassa and LCM virus) than between the new world arenaviruses (JUNV and Machupo virus).
authors.affiliation: Laboratorio de Ingeniería Genética y Biología Celular y Molecular, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Roque Sáenz Peña 352, B1876BXD, Bernal, Buenos Aires, Argentina. sangoni@gmail.com
authors.forename: Sandra E
authors.initials: SE
authors.lastname: Goñi
authors.forename: Betina I
authors.initials: BI
authors.lastname: Stephan
authors.forename: Javier A
authors.initials: JA
authors.lastname: Iserte
authors.forename: Marta S
authors.initials: MS
authors.lastname: Contigiani
authors.forename: Mario E
authors.initials: ME
authors.lastname: Lozano
authors.forename: Antonio
authors.initials: A
authors.lastname: Tenorio
mesh_terms: D000818:Animals; D001117:Arenaviridae Infections / Q000662:veterinary* / Q000821:virology*; D001118:Argentina; D016000:Cluster Analysis; D014644:Genetic Variation; D006801:Humans; D018051:Junin virus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012376:Rodent Diseases / Q000821:virology*; D012377:Rodentia; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D012367:RNA, Viral