title: Acinetobacter sp. HM746599 isolated from leatherback turtle blood.
abstract: A newly described bacterial isolate, Acinetobacter sp. HM746599, has been obtained from leatherback sea turtle hatchling blood. The implication is that the hatchling was infected during development in the egg, which is substantiated by other studies to be reported by us in the future. The 16S rRNA gene sequence of the bacterium (GenBank accession number: HM746599) showed the greatest similarity to the identified species, Acinetobacter beijerinckii (97.6-99.78%) and Acinetobacter venetianus (99.78%). Acinetobacter sp. HM746599 are gram-negative, rod-shaped coccobacilli and are hemolytic/cytotoxic to human and sea turtle red blood cells (RBCs). Hemolysis is not the result of any detectable soluble toxin. Acinetobacter beijerinckii and A. venetianus hemolyze sheep RBCs while Acinetobacter sp. HM746599 does not, and unlike A. venetianus, the growth of Acinetobacter sp. HM746599 and A. beijerinckii is not supported by l-arginine. Many Acinetobacter species, especially hemolytic ones, are pathogenic to immunologically compromised humans and it is possible that, in addition to sea turtles, this bacterium might also be a danger to susceptible humans who handle infected hatchlings. The bacteria are available from CCUG (Culture Collection, University Gothenburg, Göteborg, Sweden) and from NRRL (Agricultural Research Service Culture Collection, Peoria, IL).
authors.affiliation: Office of Professional Studies in the Health Sciences, Drexel University College of Medicine, Philadelphia, PA 19102, USA. gsoslau@drexelmed.edu
authors.forename: Gerald
authors.initials: G
authors.lastname: Soslau
authors.forename: Jacob A
authors.initials: JA
authors.lastname: Russell
authors.forename: James R
authors.initials: JR
authors.lastname: Spotila
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Mathew
authors.forename: Pamela
authors.initials: P
authors.lastname: Bagsiyao
mesh_terms: D000150:Acinetobacter / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D000151:Acinetobacter Infections / Q000382:microbiology / Q000635:transmission / Q000662:veterinary*; D000818:Animals; D015373:Bacterial Typing Techniques; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014426:Turtles / Q000382:microbiology*
chemical_list: D012336:RNA, Ribosomal, 16S