title: Development of microsatellite markers for Miscanthus sinensis (Poaceae) and cross-amplification in other related species.
abstract: OBJECTIVE
  We developed microsatellite loci for the biomass crop Miscanthus sinensis to investigate genetic diversity and population structure of M. sinensis and its closely related species.
  
  
  RESULTS
  Fourteen microsatellite loci were identified from an enriched genomic library of M. sinensis and tested in one M. sinensis population. The number of alleles per locus ranged from 1 to 15, with a mean of 7.0. The observed and expected heterozygosities varied from 0.318 to 0.864 and from 0.424 to 0.901, respectively. Of them, 12 primers could be applied to three other species in Miscanthus (M. sacchariflorus, M. floridulus, and M. lutarioriparius).
  
  
  CONCLUSIONS
  These markers will be important for further analyzing population genetics and evolutionary history, as well as facilitating molecular breeding of Miscanthus sinensis and its related species.
authors.affiliation: State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
authors.forename: Hai-Fei
authors.initials: HF
authors.lastname: Zhou
authors.forename: Shan-Shan
authors.initials: SS
authors.lastname: Li
authors.forename: Song
authors.initials: S
authors.lastname: Ge
mesh_terms: D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D006109:Poaceae / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D013045:Species Specificity