title: Genetic diversity and phylogeny of antagonistic bacteria against Phytophthora nicotianae isolated from tobacco rhizosphere.
abstract: The genetic diversity of antagonistic bacteria from the tobacco rhizosphere was examined by BOXAIR-PCR, 16S-RFLP, 16S rRNA sequence homology and phylogenetic analysis methods. These studies revealed that 4.01% of the 6652 tested had some inhibitory activity against Phytophthora nicotianae. BOXAIR-PCR analysis revealed 35 distinct amplimers aligning at a 91% similarity level, reflecting a high degree of genotypic diversity among the antagonistic bacteria. A total of 25 16S-RFLP patterns were identified representing over 33 species from 17 different genera. Our results also found a significant amount of bacterial diversity among the antagonistic bacteria compared to other published reports. For the first time; Delftia tsuruhatensis, Stenotrophomonas maltophilia, Advenella incenata, Bacillus altitudinis, Kocuria palustris, Bacillus licheniformis, Agrobacterium tumefaciens and Myroides odoratimimus are reported to display antagonistic activity towards Phytophthora nicotianae. Furthermore, the majority (75%) of the isolates assayed for antagonistic activity were Gram-positives compared to only 25% that were Gram-negative bacteria.
authors.affiliation: Shandong Key Laboratory of Agricultural Microbiology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, China; E-Mails: jfl315@163.com (F.J.); dingyq6885@163.com (Y.D.).
authors.forename: Fengli
authors.initials: F
authors.lastname: Jin
authors.forename: Yanqin
authors.initials: Y
authors.lastname: Ding
authors.forename: Wei
authors.initials: W
authors.lastname: Ding
authors.forename: M S
authors.initials: MS
authors.lastname: Reddy
authors.forename: W G Dilantha
authors.initials: WG
authors.lastname: Fernando
authors.forename: Binghai
authors.initials: B
authors.lastname: Du
mesh_terms: D044822:Biodiversity; D004269:DNA, Bacterial / Q000737:chemistry; D054884:Host-Pathogen Interactions; D010802:Phylogeny; D010838:Phytophthora / Q000502:physiology*; D012150:Polymorphism, Restriction Fragment Length; D058441:Rhizosphere; D017422:Sequence Analysis, DNA; D014026:Nicotiana / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial