title: Chaperonin containing T-complex polypeptide (CCT) subunit expression in oral mucosal wounds and fibroblasts.
abstract: Mucosal wound healing in adults has been reported to feature diminished scar formation compared to healing skin wounds. We sought to determine if the expression pattern of chaperonin containing T-complex polypeptide (CCT) subunits in mucosal wounds and fibroblasts is different from that observed in skin wounds and fibroblasts. We found that CCT-beta is the only subunit message to be reduced in wounded mucosa versus unwounded control, and this reduction was confirmed at the protein level. In contrast, mRNA levels of CCT-zeta, -delta, -eta, and -epsilon were significantly increased in mucosal wounds. The increase in CCT-eta was also confirmed at the protein level. Expression levels of CCT-alpha, -beta, -delta; -epsilon, and -theta mRNAs were significantly increased in adult mucosal fibroblasts in culture compared to skin-derived fibroblasts. Western blot analyses confirmed a modest increase in CCT-beta in adult mucosal fibroblasts relative to skin fibroblasts, but CCT-eta protein was unaffected. These differences may contribute to the reported difference in healing outcomes between these two tissue types.
authors.affiliation: Center for Genomic Sciences, Allegheny-Singer Research Institute, Allegheny General Hospital, Pittsburgh, PA 15212, USA.
authors.forename: Latha
authors.initials: L
authors.lastname: Satish
authors.forename: Nancy
authors.initials: N
authors.lastname: Lo
authors.forename: Phillip H
authors.initials: PH
authors.lastname: Gallo
authors.forename: Sandra
authors.initials: S
authors.lastname: Johnson
authors.forename: Stephanie
authors.initials: S
authors.lastname: Haberman
authors.forename: Sandeep
authors.initials: S
authors.lastname: Kathju
mesh_terms: D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D056404:Chaperonin Containing TCP-1 / Q000235:genetics*; D005347:Fibroblasts / Q000378:metabolism*; D005786:Gene Expression Regulation; D009061:Mouth Mucosa / Q000378:metabolism*; D021122:Protein Subunits / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits; D060888:Real-Time Polymerase Chain Reaction; D012867:Skin / Q000378:metabolism; D014945:Wound Healing
chemical_list: D021122:Protein Subunits; D012333:RNA, Messenger; D056404:Chaperonin Containing TCP-1