title: Pharmacokinetic and pharmacodynamic drug interactions of carbamazepine and glibenclamide in healthy albino Wistar rats.
abstract: OBJECTIVE
  To find out the pharmacokinetic and pharmacodynamic drug interaction of carbamazepine, a protype drug used to treat painful diabetic neuropathy with glibenclamide in healthy albino Wistar rats following single and multiple dosage treatment.
  
  
  METHODS
  Therapeutic doses (TD) of glibenclamide and TD of carbamazepine were administered to the animals. The blood glucose levels were estimated by GOD/POD method and the plasma glibenclamide concentrations were estimated by a sensitive RP HPLC method to calculate pharmacokinetic parameters.
  
  
  RESULTS
  In single dose study the percentage reduction of blood glucose levels and glibenclamide concentrations of rats treated with both carbamazepine and glibenclamide were significantly increased when compared with glibenclamide alone treated rats and the mechanism behind this interaction may be due to inhibition of P-glycoprotein mediated transport of glibenclamide by carbamazepine, but in multiple dose study the percentage reduction of blood glucose levels and glibenclamide concentrations were reduced and it may be due to inhibition of P-glycoprotein mediated transport and induction of CYP2C9, the enzyme through which glibenclamide is metabolised.
  
  
  CONCLUSIONS
  In the present study there is a pharmacokinetic and pharmacodynamic interaction between carbamazepine and glibenclamide was observed. The possible interaction involves both P-gp and CYP enzymes. To investigate this type of interactions pre-clinically are helpful to avoid drug-drug interactions in clinical situation.
authors.affiliation: Vaagdevi College of Pharmacy, Vishwambara Educational Society, Ramnagar, Hanmakonda, Warangal - 506 001, A.P, India.
authors.forename: S
authors.initials: S
authors.lastname: Prashanth
authors.forename: A Anil
authors.initials: AA
authors.lastname: Kumar
authors.forename: B
authors.initials: B
authors.lastname: Madhu
authors.forename: N
authors.initials: N
authors.lastname: Rama
authors.forename: J Vidya
authors.initials: JV
authors.lastname: Sagar