title: Typing of O26 enterohaemorrhagic and enteropathogenic Escherichia coli isolated from humans and cattle with IS621 multiplex PCR-based fingerprinting.
abstract: OBJECTIVE
  This study evaluated a typing method of O26:H11 enterohaemorrhagic and enteropathogenic Escherichia coli (EHEC and EPEC) based on the variation in genomic location and copy numbers of IS621.
  
  
  RESULTS
  Two multiplex PCRs, targeting either the left (5') or right (3') IS/chromosome junction of 12 IS621 insertion sites and one PCR specific of another truncated copy, were developed. Thirty-eight amplification profiles were observed amongst a collection of 69 human and bovine O26:H11 EHEC and EPEC. Seventy-one per cent of the 45 EHEC and EPEC with identical IS621 fingerprints within groups of two, three or four isolates had >85% pulsed field gel electrophoresis (PFGE) profile similarity, including four groups of epidemiologically related EHEC or EPEC, while most of the groups had <85% similarity between each others. Epidemiologically related EHEC from each of three independent outbreaks in Japan and Belgium also exhibited identical IS621 fingerprints and PFGE profiles.
  
  
  CONCLUSIONS
  The IS621 fingerprinting and the PFGE are complementary typing assays of EHEC and EPEC; though, the former is less discriminatory.
  
  
  CONCLUSIONS
  The IS621 printing method represents a rapid (24 h) first-line surveillance and typing assay, to compare and trace back O26:H11 EHEC and EPEC during surveys in farms, multiple human cases and outbreaks.
authors.affiliation: Bacteriology, Department of Infectious Diseases, Faculty of Veterinary Medicine, University of Liège, Liège, Belgium. jg.mainil@ulg.ac.be
authors.forename: J G
authors.initials: JG
authors.lastname: Mainil
authors.forename: M
authors.initials: M
authors.lastname: Bardiau
authors.forename: T
authors.initials: T
authors.lastname: Ooka
authors.forename: Y
authors.initials: Y
authors.lastname: Ogura
authors.forename: K
authors.initials: K
authors.lastname: Murase
authors.forename: Y
authors.initials: Y
authors.lastname: Etoh
authors.forename: S
authors.initials: S
authors.lastname: Ichihara
authors.forename: K
authors.initials: K
authors.lastname: Horikawa
authors.forename: G
authors.initials: G
authors.lastname: Buvens
authors.forename: D
authors.initials: D
authors.lastname: Piérard
authors.forename: T
authors.initials: T
authors.lastname: Itoh
authors.forename: T
authors.initials: T
authors.lastname: Hayashi
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques / Q000379:methods*; D001530:Belgium; D002417:Cattle; D016172:DNA Fingerprinting / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D054324:Enterohemorrhagic Escherichia coli / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D054308:Enteropathogenic Escherichia coli / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D007564:Japan; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D019081:O Antigens / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D019081:O Antigens