title: Effect of αGal on corneal xenotransplantation in a mouse model.
abstract: BACKGROUND
  It has been reported that hyperacute rejection (HAR) does not occur after pig-to-nonhuman corneal xenotransplantation. However, considering that immune privilege is already disrupted in most human corneal recipients, and the expression of αGal can be gradually reduced after pig-to-rat corneal transplantation, the long-term survival of corneal grafts from wild-type pigs cannot be guaranteed. Accordingly, we aimed to investigate the effect of αGal on the change in anti-Gal antibodies, using sensitized α1,3-galactosyltransferase gene-knockout (GTKO) mice recipients.
  
  
  METHODS
  C57BL/6 (B6) and GTKO mice were divided into 5 groups and underwent orthotopically full thickness cormeal transplantation as follows (n=4 for each group): (1) group 1: B6 to B6; (2) group 2: fresh porcine posterior corneal lamella to B6; (3) fresh porcine posterior corneal lamella to GTKO; (4) group 4: decellularized porcine posterior corneal lamella to GTKO, and (5) group 5: B6 to GTKO. Before transplantation, all GTKO recipients were sensitized using intraperitoneal injections of rabbit blood cells. Median survival times (MST) for the corneal grafts of the different groups were compared and plasma concentrations of IgG/IgM anti-Gal antibodies were evaluated at 1 week, 2 weeks and 3 weeks post-transplantation.
  
  
  RESULTS
  There were no differences in MSTs between groups. Although there was no HAR of fresh porcine posterior corneal grafts even in sensitized GTKO recipients, αGal expression was induced in the transplanted fresh porcine corneal grafts and plasma concentration of IgG anti-Gal antibody was gradually increased in fresh porcine cornea-grafted GTKO recipients. On the contrary, αGal expression did not increase in the grafts and plasma concentration of anti-Gal antibodies did not change after transplantation using decellularized porcine corneas.
  
  
  CONCLUSIONS
  Our findings suggest that αGal may affect the long-term survival of porcine corneal xenografts via antibody-mediated rejection, although αGal does not have an effect on acute rejection and decellularized porcine corneas may enable the long-term survival of porcine corneal xenografts.
authors.affiliation: Department of Ophthalmology, Healthcare System Gangnam Center, Seoul National University Hospital, 101 Daehang-ro, Jongno-gu, Seoul, Republic of Korea.
authors.forename: Hyuk Jin
authors.initials: HJ
authors.lastname: Choi
authors.forename: Mee Kum
authors.initials: MK
authors.lastname: Kim
authors.forename: Hyun Ju
authors.initials: HJ
authors.lastname: Lee
authors.forename: So Hee
authors.initials: SH
authors.lastname: Jeong
authors.forename: Hee Jung
authors.initials: HJ
authors.lastname: Kang
authors.forename: Chan-Sik
authors.initials: CS
authors.lastname: Park
authors.forename: Chung-Gyu
authors.initials: CG
authors.lastname: Park
authors.forename: Sang
authors.initials: S
authors.lastname: Joon Kim
authors.forename: Won Ryang
authors.initials: WR
authors.lastname: Wee
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D016039:Corneal Transplantation; D005697:Galactosides / Q000378:metabolism; D005700:Galactosyltransferases / Q000235:genetics / Q000378:metabolism*; D006084:Graft Rejection / Q000276:immunology; D006085:Graft Survival / Q000276:immunology; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D011817:Rabbits; D051381:Rats; D013552:Swine; D014183:Transplantation, Heterologous / Q000276:immunology*
chemical_list: D000906:Antibodies; D005697:Galactosides; D005700:Galactosyltransferases; C047006:N-acetyllactosaminide alpha-1,3-galactosyltransferase