title: Biomarkers of disease and post-mortem changes - Heat stabilization, a necessary tool for measurement of protein regulation.
abstract: This review focuses on post sampling changes and how the Stabilizor system has been used to control this natural biological process and potential implications on cancer-specific biomarkers due to post sampling changes. Tissue sampling is a major traumatic event that can have drastic effects within a very short timeframe at the molecular level [1] resulting in loss of sample quality due to post-mortem changes. A heat-stabilization technology, using the Stabilizor system, has been developed to quickly and permanently abolish the enzymatic activity that causes these changes post-sampling and so preserve sample quality. The Stabilizor system has been shown to give better sample quality when analyzing a variety of tissues in various proteomic workflows. In this paper we discuss the impact of using heat-stabilized tissue in different proteomic applications. Based on our observations regarding the overlap between commonly changing proteins and proteins found to change post-mortem we also highlight a group of proteins of particular interest in cancer studies.
authors.affiliation: Analytical Chemistry, Department of Physical and Analytical Chemistry, Uppsala University, 75124, Uppsala, Sweden.
authors.forename: Kim
authors.initials: K
authors.lastname: Kultima
authors.forename: Karl
authors.initials: K
authors.lastname: Sköld
authors.forename: Mats
authors.initials: M
authors.lastname: Borén
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D004194:Disease / Q000145:classification; D006358:Hot Temperature; D006801:Humans; D011180:Postmortem Changes; D055550:Protein Stability; D011506:Proteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D040901:Proteomics / Q000379:methods*
chemical_list: D015415:Biomarkers; D011506:Proteins