title: Exogenous JH and ecdysteroid applications alter initiation of polydnaviral replication in an endoparasitoid wasp, Cotesia plutellae (Braconidae: Hymenoptera).
abstract: Polydnaviruses are a group of double-stranded DNA viruses and are symbiotically associated with some ichneumonoid wasps. As proviruses, the replication of polydnaviruses occurs in the female reproductive organ at the pupal stage. This study analyzed the effects of two developmental hormones, juvenile hormone (JH) and ecdysteroid, on the viral replication of Cotesia plutellae bracovirus (CpBV). All 23 CpBV segments identified contained a conserved excision/rejoining site ('AGCTTT') from their proviral segments. Using quantitative real-time PCR based on this excision/rejoining site marker, initiation of CpBV replication was determined to have occurred on day 4 on the pupal stage. Pyriproxyfen, a JH agonist, significantly inhibited adult emergence of C. plutellae, whereas RH5992, an ecdysteroid agonist, had no inhibitory effect. Although RH5992 had no effect dose on adult development, it significantly accelerated viral replication. The results of immunoblotting assays against viral coat proteins support the effects of the hormone agonists on viral replication.
authors.affiliation: Department of Bioresource Sciences, Andong National University, Andong 760-749, Korea.
authors.forename: Bokri
authors.initials: B
authors.lastname: Park
authors.forename: Yonggyun
authors.initials: Y
authors.lastname: Kim
mesh_terms: D000818:Animals; D001483:Base Sequence; D026461:Ecdysteroids / Q000819:agonists / Q000494:pharmacology*; D005260:Female; D007605:Juvenile Hormones / Q000819:agonists / Q000494:pharmacology*; D008969:Molecular Sequence Data; D018071:Polydnaviridae / Q000187:drug effects* / Q000235:genetics / Q000502:physiology*; D011679:Pupa / Q000166:cytology / Q000821:virology; D016415:Sequence Alignment; D014764:Viral Proteins / Q000378:metabolism; D014779:Virus Replication / Q000187:drug effects*; D014863:Wasps / Q000033:anatomy & histology / Q000502:physiology / Q000821:virology*
chemical_list: D026461:Ecdysteroids; D007605:Juvenile Hormones; D014764:Viral Proteins