title: Purification and characterization of myosin-tripolyphosphatase from rabbit Psoas major muscle: research note.
abstract: In this study, we investigated the tripolyphosphatase (TPPase) activity responsible for the hydrolysis of tripolyphosphates (TPP) in rabbit Psoas major muscle tissue. After a series of extraction and purification steps, myosin was identified to be a TPPase. Optimum pH and temperature for myosin-TPPase activity were 6.0 and 35°C, respectively. We also found that myosin-TPPase activity was significantly influenced by Mg(2+) and Ca(2+) levels, whose optimal concentrations were determined to be 3 and 6mM, respectively. Furthermore, myosin-TPPase was strongly inhibited by EDTA-4Na(+) and KIO(3), and was slightly activated by EDTA-2Na(+). These results suggest that it may be useful to regulate tripolyphosphate hydrolysis to enhance its function in meat processing.
authors.affiliation: Department of Food Science and Technology, Nanjing Agricultural University, Nanjing, 210095, PR China.
authors.forename: Hongguo
authors.initials: H
authors.lastname: Jin
authors.forename: Youling
authors.initials: Y
authors.lastname: Xiong
authors.forename: Zengqi
authors.initials: Z
authors.lastname: Peng
authors.forename: Yan
authors.initials: Y
authors.lastname: He
authors.forename: Rongrong
authors.initials: R
authors.lastname: Wang
authors.forename: Guanghong
authors.initials: G
authors.lastname: Zhou
mesh_terms: D017766:Acid Anhydride Hydrolases / Q000737:chemistry* / Q000302:isolation & purification*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D011756:Diphosphates / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004791:Enzyme Inhibitors / Q000378:metabolism; D005260:Female; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008274:Magnesium / Q000378:metabolism; D009124:Muscle Proteins / Q000737:chemistry / Q000302:isolation & purification*; D009218:Myosins / Q000737:chemistry* / Q000302:isolation & purification; D011122:Polyphosphates / Q000378:metabolism; D016658:Psoas Muscles / Q000201:enzymology*; D011817:Rabbits; D013696:Temperature
chemical_list: D011756:Diphosphates; D004791:Enzyme Inhibitors; D009124:Muscle Proteins; D011122:Polyphosphates; D017766:Acid Anhydride Hydrolases; C023193:tripolyphosphatase; D009218:Myosins; D008274:Magnesium; C005692:triphosphoric acid; C003319:sodium pyrophosphate; D002118:Calcium