title: The trophoblast binucleate cell is the source of maternal circulating C-type natriuretic peptide during ovine pregnancy.
abstract: Maternal plasma concentrations of C-type natriuretic peptide (CNP) and a co-secreted bioinactive amino-terminal fragment (NTproCNP) are elevated during ovine pregnancy. Although the uteroplacental unit has been implicated as a likely source of CNP, the relative contributions of specific uterine and placental tissues, and identity of the cellular site/s of production remain unknown. Therefore, we measured CNP and NTproCNP in intercaruncular uterine tissue and maternal (caruncle) and fetal (cotyledon) placental tissues throughout gestation. Concentrations of CNP forms in placental tissues greatly exceeded those in intercaruncular uterine tissue throughout pregnancy (P < 0.05). Mean caruncular concentrations (CNP 32 ± 4, NTproCNP 56 ± 6 pmol g(-1)) peaked at day 60 whereas in the cotyledon there was a progressive increase in CNP forms to peak values (CNP 66 ± 6, NTproCNP 134 ± 9 pmol g(-1)) at day 100-135 followed by a sharp decline just prior to term (day 143). At term CNP gene expression was 6-fold greater in placental tissue compared with intercaruncular uterine tissue. Changes in maternal plasma concentration of CNP forms closely followed those in cotyledonary tissue whereas fetal plasma levels fell progressively throughout gestation. Immunohistochemistry revealed staining in binucleate cells (BNC) and around placental blood vessels. CNP's localization to the BNC suggests a novel endocrine role during pregnancy, in addition to its paracrine actions within the placental vasculature. The function of CNP in maternal circulation remains to be determined, but as proposed for other BNC products, may involve manipulation of maternal physiology and placental function to favour fetal growth.
authors.affiliation: Faculty of Agriculture and Life Sciences, Lincoln University, Christchurch, New Zealand. Electronic address: bryony.mcneill@lincolnuni.ac.nz.
authors.forename: B A
authors.initials: BA
authors.lastname: McNeill
authors.affiliation: Faculty of Agriculture and Life Sciences, Lincoln University, Christchurch, New Zealand.
authors.forename: G K
authors.initials: GK
authors.lastname: Barrell
authors.affiliation: Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, United Kingdom.
authors.forename: F B P
authors.initials: FBP
authors.lastname: Wooding
authors.affiliation: Department of Medicine, University of Otago, Christchurch, New Zealand.
authors.forename: T C R
authors.initials: TCR
authors.lastname: Prickett
authors.affiliation: Department of Medicine, University of Otago, Christchurch, New Zealand.
authors.forename: E A
authors.initials: EA
authors.lastname: Espiner
mesh_terms: D000818:Animals; D005260:Female; D020098:Natriuretic Peptide, C-Type / Q000097:blood*; D010920:Placenta / Q000378:metabolism; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000502:physiology*; D011498:Protein Precursors / Q000097:blood; D034561:Sheep, Domestic; D014327:Trophoblasts / Q000166:cytology* / Q000378:metabolism; D014599:Uterus / Q000378:metabolism
chemical_list: D011498:Protein Precursors; D020098:Natriuretic Peptide, C-Type