title: Characterization of the expression profile of calpain-3 (CAPN3) gene in chicken.
abstract: Calpain-3 is a skeletal muscle-specific protease and participates in the regulation of myogenesis. In this study, we quantified the expression of calpain-3 (CAPN3) mRNA in a Chinese local chicken breed (Sichuan Mountainous Black-boned chicken [MB]), to discern the tissue and ontogenic expression pattern. Meanwhile, we compared the CAPN3 mRNA expression pattern in MB chicken at 10 weeks with a commercial meat type chicken line (S01) of the same age to identify the unique expression pattern under different genetic background. A real time quantitative PCR (qRT-PCR) assay was developed for an accurate measurement of its expression in various tissues from chickens at different ages (0, 2, 4, 6, 8, 10, and 12 weeks). Expression of the CAPN3 mRNA was detected in the selected tissues, regardless of age. The breast muscle and leg muscle tissues had a significantly higher expression than the other tissues from the same individual (P < 0.01). Overall, the CAPN3 mRNA level exhibited a "rise-decline" developmental change in detected tissues except for brain. The S01 chicken had a higher expression of the CAPN3 mRNA in detected tissues than the MB chicken at 10 weeks. The present expression data of chicken CAPN3 gene may provide some information to shed light on the tissue and ontogenic expression pattern during chicken development.
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, 46# Xinkang Road, Ya'an, Sichuan, 625014, China.
authors.forename: Zeng-Rong
authors.initials: ZR
authors.lastname: Zhang
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhu
authors.forename: Yong-Gang
authors.initials: YG
authors.lastname: Yao
authors.forename: Xiao-Song
authors.initials: XS
authors.lastname: Jiang
authors.forename: Hua-Rui
authors.initials: HR
authors.lastname: Du
authors.forename: Yi-Ping
authors.initials: YP
authors.lastname: Liu
mesh_terms: D000818:Animals; D001947:Breeding; D002154:Calpain / Q000235:genetics / Q000378:metabolism; D002645:Chickens / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D008297:Male; D009928:Organ Specificity / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D012333:RNA, Messenger; D002154:Calpain