title: Regulation of the IL-10/IL-12 axis in human dendritic cells with probiotic bacteria.
abstract: In this study, we have used monocyte-derived dendritic cells (DCs) to design a screening model for the selection of microorganisms with the ability to suppress DC-secreted IL-12p70, a critical cytokine for the induction of T-helper cell type 1 immune responses under inflammatory conditions. By the treatment of DCs with cocktails containing TLR agonists and proinflammatory cytokines, the cells increased the secretion of the Th1-promoting cytokine IL-12p70. Clinically used probiotics were tested for their IL-10- and IL-12p70-stimulating properties in immature DCs, and showed a dose-dependent change in the IL-10/IL-12p70 balance. Lactobacillus acidophilus NCFM(™) and the probiotic mixture VSL#3 showed a strong induction of IL-12p70, whereas Lactobacillus salivarius Ls-33 and Bifidobacterium infantis 35624 preferentially induced IL-10. Escherichia coli Nissle 1917 induced both IL-10 and IL-12p70, whereas the probiotic yeast Saccharomyces boulardii induced low levels of cytokines. When combining these microorganisms with the Th1-promoting cocktails, E. coli Nissle 1917 and B. infantis 35624 were potent suppressors of IL-12p70 secretion in an IL-10-independent manner, indicating a suppressive effect on Th1-inducing antigen-presenting cells. The present model, using cocktail-stimulated DCs with potent IL-12p70-stimulating capacity, may be used as an efficient tool to assess the anti-inflammatory properties of microorganisms for potential clinical use.
authors.affiliation: Bioneer A/S, Kogle Allé 2, Hørsholm, Denmark.
authors.forename: Monika
authors.initials: M
authors.lastname: Gad
authors.forename: Peter
authors.initials: P
authors.lastname: Ravn
authors.forename: Ditte A
authors.initials: DA
authors.lastname: Søborg
authors.forename: Karina
authors.initials: K
authors.lastname: Lund-Jensen
authors.forename: Arthur C
authors.initials: AC
authors.lastname: Ouwehand
authors.forename: Simon S
authors.initials: SS
authors.lastname: Jensen
mesh_terms: D001419:Bacteria / Q000276:immunology*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D016753:Interleukin-10 / Q000096:biosynthesis*; D018664:Interleukin-12 / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D019936:Probiotics / Q000494:pharmacology*; D012440:Saccharomyces / Q000276:immunology*
chemical_list: C508609:IL10 protein, human; D016753:Interleukin-10; D018664:Interleukin-12