title: Identification of pectin methylesterase 3 as a basic pectin methylesterase isoform involved in adventitious rooting in Arabidopsis thaliana.
abstract: • Here, we focused on the biochemical characterization of the Arabidopsis thaliana pectin methylesterase 3 gene (AtPME3; At3g14310) and its role in plant development. • A combination of biochemical, gene expression, Fourier transform-infrared (FT-IR) microspectroscopy and reverse genetics approaches were used. • We showed that AtPME3 is ubiquitously expressed in A. thaliana, particularly in vascular tissues. In cell wall-enriched fractions, only the mature part of the protein was identified, suggesting that it is processed before targeting the cell wall. In all the organs tested, PME activity was reduced in the atpme3-1 mutant compared with the wild type. This was related to the disappearance of an activity band corresponding to a pI of 9.6 revealed by a zymogram. Analysis of the cell wall composition showed that the degree of methylesterification (DM) of galacturonic acids was affected in the atpme3-1 mutant. A change in the number of adventitious roots was found in the mutant, which correlated with the expression of the gene in adventitious root primordia. • Our results enable the characterization of AtPME3 as a major basic PME isoform in A. thaliana and highlight its role in adventitious rooting.
authors.affiliation: EA3900-BioPI Biologie des Plantes et Contrôle des Insectes Ravageurs, Université de Picardie, 33 Rue St Leu, F-80039 Amiens, France.|CRRBM - Bâtiment Serres Transfert, Université de Picardie Jules Verne, 33 Rue St Leu, F-80039 Amiens, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Guénin
authors.affiliation: Laboratoire 'Glycobiologie et Matrice Extracellulaire Végétale' UPRES-EA 4358, IFRMP 23, UFR des Sciences et Techniques, F-76821 Mont-Saint-Aignan, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Mareck
authors.affiliation: EA3900-BioPI Biologie des Plantes et Contrôle des Insectes Ravageurs, Université de Picardie, 33 Rue St Leu, F-80039 Amiens, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Rayon
authors.affiliation: Laboratoire 'Glycobiologie et Matrice Extracellulaire Végétale' UPRES-EA 4358, IFRMP 23, UFR des Sciences et Techniques, F-76821 Mont-Saint-Aignan, France.
authors.forename: Romain
authors.initials: R
authors.lastname: Lamour
authors.affiliation: EA3900-BioPI Biologie des Plantes et Contrôle des Insectes Ravageurs, Université de Picardie, 33 Rue St Leu, F-80039 Amiens, France.
authors.forename: Yves
authors.initials: Y
authors.lastname: Assoumou Ndong
authors.affiliation: EA3900-BioPI Biologie des Plantes et Contrôle des Insectes Ravageurs, Université de Picardie, 33 Rue St Leu, F-80039 Amiens, France.
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Domon
authors.affiliation: EA3900-BioPI Biologie des Plantes et Contrôle des Insectes Ravageurs, Université de Picardie, 33 Rue St Leu, F-80039 Amiens, France.
authors.forename: Fabien
authors.initials: F
authors.lastname: Sénéchal
authors.affiliation: EA3900-BioPI Biologie des Plantes et Contrôle des Insectes Ravageurs, Université de Picardie, 33 Rue St Leu, F-80039 Amiens, France.
authors.forename: Françoise
authors.initials: F
authors.lastname: Fournet
authors.affiliation: UPS, CNRS, UMR 5546 Surfaces Cellulaires et Signalisation chez les Végétaux, Université de Toulouse, BP42617, F-31326 Castanet-Tolosan, France.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Jamet
authors.affiliation: UPS, CNRS, UMR 5546 Surfaces Cellulaires et Signalisation chez les Végétaux, Université de Toulouse, BP42617, F-31326 Castanet-Tolosan, France.
authors.forename: Hervé
authors.initials: H
authors.lastname: Canut
authors.affiliation: Laboratoire 'Glycobiologie et Matrice Extracellulaire Végétale' UPRES-EA 4358, IFRMP 23, UFR des Sciences et Techniques, F-76821 Mont-Saint-Aignan, France.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Percoco
authors.affiliation: Institut Jean-Pierre Bourgin, UMR1318 INRA-AgroParisTech, Bâtiment 2, INRA Centre de Versailles-Grignon, Route de St Cyr (RD 10), F-78026 Versailles Cedex France.
authors.forename: Grégory
authors.initials: G
authors.lastname: Mouille
authors.affiliation: Institut Jean-Pierre Bourgin, UMR1318 INRA-AgroParisTech, Bâtiment 2, INRA Centre de Versailles-Grignon, Route de St Cyr (RD 10), F-78026 Versailles Cedex France.
authors.forename: Aurélia
authors.initials: A
authors.lastname: Rolland
authors.affiliation: EA3900-BioPI Biologie des Plantes et Contrôle des Insectes Ravageurs, Université de Picardie, 33 Rue St Leu, F-80039 Amiens, France.
authors.forename: Christine
authors.initials: C
authors.lastname: Rustérucci
authors.affiliation: EA3900-BioPI Biologie des Plantes et Contrôle des Insectes Ravageurs, Université de Picardie, 33 Rue St Leu, F-80039 Amiens, France.
authors.forename: François
authors.initials: F
authors.lastname: Guerineau
authors.affiliation: EA3900-BioPI Biologie des Plantes et Contrôle des Insectes Ravageurs, Université de Picardie, 33 Rue St Leu, F-80039 Amiens, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Van Wuytswinkel
authors.affiliation: EA3900-BioPI Biologie des Plantes et Contrôle des Insectes Ravageurs, Université de Picardie, 33 Rue St Leu, F-80039 Amiens, France.
authors.forename: Françoise
authors.initials: F
authors.lastname: Gillet
authors.affiliation: Laboratoire 'Glycobiologie et Matrice Extracellulaire Végétale' UPRES-EA 4358, IFRMP 23, UFR des Sciences et Techniques, F-76821 Mont-Saint-Aignan, France.
authors.forename: Azeddine
authors.initials: A
authors.lastname: Driouich
authors.affiliation: Laboratoire 'Glycobiologie et Matrice Extracellulaire Végétale' UPRES-EA 4358, IFRMP 23, UFR des Sciences et Techniques, F-76821 Mont-Saint-Aignan, France.
authors.forename: Patrice
authors.initials: P
authors.lastname: Lerouge
authors.affiliation: CRRBM - Bâtiment Serres Transfert, Université de Picardie Jules Verne, 33 Rue St Leu, F-80039 Amiens, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Gutierrez
authors.affiliation: EA3900-BioPI Biologie des Plantes et Contrôle des Insectes Ravageurs, Université de Picardie, 33 Rue St Leu, F-80039 Amiens, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Pelloux
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology* / Q000254:growth & development*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000378:metabolism*; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry / Q000378:metabolism*; D002473:Cell Wall / Q000201:enzymology; D004789:Enzyme Activation; D004951:Esterification; D007527:Isoenzymes / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D010368:Pectins / Q000378:metabolism; D010641:Phenotype; D018517:Plant Roots / Q000201:enzymology* / Q000254:growth & development*; D058526:Plant Vascular Bundle / Q000201:enzymology; D011401:Promoter Regions, Genetic / Q000235:genetics; D021381:Protein Transport
chemical_list: D029681:Arabidopsis Proteins; D007527:Isoenzymes; D010368:Pectins; D002265:Carboxylic Ester Hydrolases; C568893:pectin methylesterase 3, Arabidopsis; C022323:pectinesterase