title: ALK, the key gene for gelatinization temperature, is a modifier gene for gel consistency in rice.
abstract: Gelatinization temperature (GT) is an important parameter in evaluating the cooking and eating quality of rice. Indeed, the phenotype, biochemistry and inheritance of GT have been widely studied in recent times. Previous map-based cloning revealed that GT was controlled by ALK gene, which encodes a putative soluble starch synthase II-3. Complementation vector and RNAi vector were constructed and transformed into Nipponbare mediated by Agrobacterium. Phenotypic and molecular analyses of transgenic lines provided direct evidence for ALK as a key gene for GT. Meanwhile, amylose content, gel consistency and pasting properties were also affected in transgenic lines. Two of four nonsynonymous single nucleotide polymorphisms in coding sequence of ALK were identified as essential for GT. Based on the single nucleotide polymorphisms (SNPs), two new sets of SNP markers combined with one cleaved amplified polymorphic sequence marker were developed for application in rice quality breeding.
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Science, Hangzhou 310006, China.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Gao
authors.forename: Dali
authors.initials: D
authors.lastname: Zeng
authors.forename: Fangmin
authors.initials: F
authors.lastname: Cheng
authors.forename: Zhixi
authors.initials: Z
authors.lastname: Tian
authors.forename: Longbiao
authors.initials: L
authors.lastname: Guo
authors.forename: Yan
authors.initials: Y
authors.lastname: Su
authors.forename: Meixian
authors.initials: M
authors.lastname: Yan
authors.forename: Hua
authors.initials: H
authors.lastname: Jiang
authors.forename: Guojun
authors.initials: G
authors.lastname: Dong
authors.forename: Yuchen
authors.initials: Y
authors.lastname: Huang
authors.forename: Bin
authors.initials: B
authors.lastname: Han
authors.forename: Jiayang
authors.initials: J
authors.lastname: Li
authors.forename: Qian
authors.initials: Q
authors.lastname: Qian
mesh_terms: D000468:Alkalies; D003433:Crosses, Genetic; D005782:Gels / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics*; D006239:Haplotypes / Q000235:genetics; D012275:Oryza / Q000201:enzymology / Q000235:genetics*; D010641:Phenotype; D030821:Plants, Genetically Modified; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D019655:Quantitative Trait, Heritable; D034622:RNA Interference; D013214:Starch Synthase / Q000235:genetics / Q000378:metabolism; D013696:Temperature; D019076:Transgenes / Q000235:genetics; D014783:Viscosity
chemical_list: D000468:Alkalies; D005782:Gels; D013214:Starch Synthase