title: Transmission electron microscopy of amyloid fibrils.
abstract: Transmission Electron Microscopy of negatively stained and cryo-prepared specimens allows amyloid fibrils to be visualised at high resolution in a dried or a hydrated state, and is an essential method for characterising the morphology of fibrils and pre-fibrillar species. We outline the key steps involved in the preparation and observation of samples using negative staining and cryo-electron preservation. We also discuss methods to measure fibril characteristics, such as fibril width, from electron micrographs.
authors.affiliation: Department of Chemical and Biomolecular Engineering, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, VIC, Australia. sgras@unimelb.edu.au
authors.forename: Sally L
authors.initials: SL
authors.lastname: Gras
authors.forename: Lynne J
authors.initials: LJ
authors.lastname: Waddington
authors.forename: Kenneth N
authors.initials: KN
authors.lastname: Goldie
mesh_terms: D000682:Amyloid / Q000737:chemistry*; D016477:Artifacts; D015925:Cryopreservation; D007091:Image Processing, Computer-Assisted; D046529:Microscopy, Electron, Transmission / Q000295:instrumentation / Q000379:methods*; D055503:Protein Multimerization; D017433:Protein Structure, Secondary
chemical_list: D000682:Amyloid