title: The Garz Sec7 domain guanine nucleotide exchange factor for Arf regulates salivary gland development in Drosophila.
abstract: Surface delivery of proteins involved in cell-cell and cell-matrix interactions in cultured mammalian cells requires the GBF1 guanine nucleotide exchange factor. However, the role of GBF1 in delivery of adhesion proteins during organogenesis in intact animals has not been characterized. Here, we report the function of the fly GBF1 homolog, Gartenzwerg (Garz) in the development of the salivary gland in Drosophila melanogaster. We used the GAL4/UAS system to selectively deplete Garz from salivary gland cells. We show that depletion of Garz disrupts the secretory pathway as evidenced by the collapse of Golgi-localized Lava lamp (Lva) and the TGN-localized γ subunit of the clathrin-adaptor protein complex (AP-1). Additionally, Garz depletion inhibits trafficking of cell-cell adhesion proteins cadherin (DE-cad) and Flamingo to the cell surface. Disregulation of trafficking correlates with mistargeting of the tumor suppressor protein Discs large involved in epithelial polarity determination. Garz-depleted salivary cells are smaller and lack well-defined plasma membrane domains. Garz depletion also inhibits normal elongation and positioning of epithelial cells, resulting in a disorganized salivary gland that lacks a well defined luminal duct. Our findings suggest that Garz is essential for establishment of epithelial structures and demonstrate an absolute requirement for Garz during Drosophila development.
authors.affiliation: Department of Cell Biology; University of Alabama at Birmingham; Birmingham, AL USA.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Szul
authors.forename: Jason
authors.initials: J
authors.lastname: Burgess
authors.forename: Mili
authors.initials: M
authors.lastname: Jeon
authors.forename: Kai
authors.initials: K
authors.lastname: Zinn
authors.forename: Guillermo
authors.initials: G
authors.lastname: Marques
authors.forename: Julie A
authors.initials: JA
authors.lastname: Brill
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Sztul