title: [Pollen vigor and development of germplasm of Dendrobium officinale].
abstract: OBJECTIVE
  To provide the theoretical basis and applied technology for breeding superior species of Dendrobium officinale.
  
  
  METHODS
  The peroxidase solution was used to test the pollen vigor in different flowering time and storage conditions. Cross, self and opening pollination were conducted in the green house, the subsidiary pollination by insects was carried out outdoors.
  
  
  RESULTS
  The pollen of D. officinale was still in vigor when the flower faded. The pollen vigor was only 29.4% in the buds, 70.6% in the bloom day, and decreased to 31.9% a week later, it remained still 21.2% 20 days later under the condition of dry and 4 degrees C. The fructification rate was about 82.6% and 7.3%, respectively, when the cross and self pollination performed by hand in the whole flowering time, the rate was 0 in the green house and outdoors.
  
  
  CONCLUSIONS
  The pollen of D. officinale was still in vigor during the whole flowering time, the fructification rate was 0 in the green house and outdoors resulted from the specific structure of flower. The wild resources of D. officinale were protected and germplasm was developed effectively through the artificial cross pollination.
authors.affiliation: Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Research and Development Center for Natural Medicine, Zhejiang Agricultural and Forestry University, Lin'an 311300, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhu
authors.forename: He
authors.initials: H
authors.lastname: Yuan
authors.forename: Qiaoxian
authors.initials: Q
authors.lastname: Yu
authors.forename: Jinping
authors.initials: J
authors.lastname: Si
mesh_terms: D031667:Dendrobium / Q000254:growth & development*; D005638:Fruit / Q000254:growth & development; D011058:Pollen / Q000502:physiology*; D054817:Pollination