title: Determination of cobalt, nickel and iron at trace level in natural water samples by in-column chelation-reversed phase high-performance liquid chromatography.
abstract: This paper reports the utilization of 4-(2-pyridylazo) resorcinol (PAR) as a chelating reagent for in-column derivatization and the determination of trace Co, Fe, and Ni ions by reversed-phase high-performance liquid chromatography with photodiode array detector. A good separation of Co, Fe, and Ni chelates were achieved by using an Inertsil ODS-3 column and a mobile phase, consisted of methanol-THF-water mixture (50:5:45) containing ammonium acetate buffer (pH 5.0) and PAR. After full optimization, good repeatability of retention times (relative standard deviation (RSD) < 0.05%) and peak areas (RSD < 1.7%) was achieved as well as a good linearity (r (2) > 0.9991). The detection limits (S/N = 3), expressed as micrograms per liter, were 0.50 (Co), 9.07 (Fe), and 2.00 (Ni). The applicability and the accuracy of the developed method were estimated by the analysis of spiked water samples and certified reference material BCR 715 wastewater-SRM.
authors.affiliation: Department of Chemistry, Faculty of Science and Art, Pamukkale University, 20017 Denizli, Turkey.
authors.forename: Aysen
authors.initials: A
authors.lastname: Hol
authors.forename: Umit
authors.initials: U
authors.lastname: Divrikli
authors.forename: Latif
authors.initials: L
authors.lastname: Elci
mesh_terms: D002614:Chelating Agents / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D056148:Chromatography, Reverse-Phase; D003035:Cobalt / Q000032:analysis / Q000737:chemistry; D004784:Environmental Monitoring / Q000379:methods; D007501:Iron / Q000032:analysis / Q000737:chemistry; D019216:Metals, Heavy / Q000032:analysis* / Q000737:chemistry; D009532:Nickel / Q000032:analysis / Q000737:chemistry; D012118:Resorcinols / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000737:chemistry
chemical_list: D002614:Chelating Agents; D019216:Metals, Heavy; D012118:Resorcinols; D014874:Water Pollutants, Chemical; D003035:Cobalt; D009532:Nickel; D007501:Iron; C028398:4-(2-pyridylazo)resorcinol