title: Targeted delivery in breast cancer cells via iodine: nuclear localization sequence conjugate.
abstract: The nonviral vector with iodine-nuclear localization sequence (namely, NLS-I) targeting breast cancer cells was fabricated. Ternary complexes were formed via charge interactions among NLS-I peptides, PEI 1800, and DNA, and we investigated their cellular internalization, nuclear accumulation as well as transfection efficiency. All the experiments were assessed by employing MCF-7 cells that express sodium/iodide symporter and HeLa cells that lack the expression of the symporter. In MCF-7 cells, cell internalization and nuclear accumulation of NLS-I was markedly increased compared to that in NLS. In addition, compared to that of the PEI1800/DNA complex, PEI1800/DNA/NLS-I complexes exhibited much enhanced luciferase reporter gene expression by up to 130-fold. By contrast, in HeLa cells, the evident improvements of cellular internalization, nuclear accumulation, and transfection efficiency by NLS-I were not observed. This study demonstrates an alternative method to construct a nonviral delivery system for targeted gene transfer into breast cancer cells.
authors.affiliation: Key Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
authors.forename: Hui-Yuan
authors.initials: HY
authors.lastname: Wang
authors.forename: Cao
authors.initials: C
authors.lastname: Li
authors.forename: Wen-Jie
authors.initials: WJ
authors.lastname: Yi
authors.forename: Yun-Xia
authors.initials: YX
authors.lastname: Sun
authors.forename: Si-Xue
authors.initials: SX
authors.lastname: Cheng
authors.forename: Ren-Xi
authors.initials: RX
authors.lastname: Zhuo
authors.forename: Xian-Zheng
authors.initials: XZ
authors.lastname: Zhang
mesh_terms: D021581:Active Transport, Cell Nucleus; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000473:pathology; D045744:Cell Line, Tumor; D004247:DNA / Q000493:pharmacokinetics / Q000627:therapeutic use; D005260:Female; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D007455:Iodine / Q000378:metabolism* / Q000493:pharmacokinetics / Q000627:therapeutic use; D019913:Nuclear Localization Signals / Q000378:metabolism / Q000493:pharmacokinetics* / Q000627:therapeutic use; D011094:Polyethyleneimine / Q000493:pharmacokinetics / Q000627:therapeutic use; D027981:Symporters / Q000378:metabolism; D014162:Transfection / Q000379:methods*; D000099209:Sodium-Iodide Symporters
chemical_list: D004247:DNA; D007455:Iodine; D019913:Nuclear Localization Signals; D011094:Polyethyleneimine; D027981:Symporters; D000099209:Sodium-Iodide Symporters