title: Contribution of Ldace1 gene to acetylcholinesterase activity in Colorado potato beetle.
abstract: The Colorado potato beetle (CPB), Leptinotarsa decemlineata is an important economic pest of potato worldwide. Resistance to organophosphates and carbamates in CPB has been associated in some cases to point mutations in the acetylcholinesterase (AChE) gene Ldace2, an orthologue of Drosophila melanogaster Dmace2. In this paper we report cloning and sequencing of Ldace1, an orthologue of Anopheles gambiae Agace1 that was previously unknown in CPB. The Ldace1 coding enzyme contains all residues conserved in a functionally active AChE. Ldace1 is expressed at higher levels (between 2- and 11-fold) than Ldace2 in embryos, in the four larval instars and in adults. Specific interference of Ldace1 by means of dsRNA injection resulted in a reduction of AChE activity to an approximate 50% compared to control, whilst interference of Ldace2 reduced AChE activity to an approximate 85%. Analysis of zymograms of AChE activity after interference indicates that LdAChE1 is the enzyme predominantly responsible for the activity visualised. Interference of Ldace1 in CPB adults caused a significant increase in mortality (43%) as early as three days post-injection (p.i.), suggesting the essential role of Ldace1. Interference of Ldace2 also caused a significant increase in mortality (29%) compared to control, although at seven days p.i. The effect of the interference of Ldace1 on susceptibility to the organophosphate chlorpyrifos points out that LdAChE1 could be a main target for this insecticide. In the light of our results, studies associating resistance in CPB to mutations in Ldace2 should be reviewed, taking into consideration analysis of the Ldace1 gene.
authors.affiliation: Dpto. de Biología Medioambiental, Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040 Madrid, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Revuelta
authors.forename: F
authors.initials: F
authors.lastname: Ortego
authors.forename: J R
authors.initials: JR
authors.lastname: Díaz-Ruíz
authors.forename: P
authors.initials: P
authors.lastname: Castañera
authors.forename: F
authors.initials: F
authors.lastname: Tenllado
authors.forename: P
authors.initials: P
authors.lastname: Hernández-Crespo
mesh_terms: D000110:Acetylcholinesterase / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D002800:Cholinesterase Inhibitors; D001517:Coleoptera / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D004591:Electrophoresis, Polyacrylamide Gel; D015870:Gene Expression; D007305:Insecticide Resistance; D007306:Insecticides; D008969:Molecular Sequence Data; D010755:Organophosphates; D010641:Phenotype; D034622:RNA Interference; D017422:Sequence Analysis, DNA
chemical_list: D002800:Cholinesterase Inhibitors; D007306:Insecticides; D010755:Organophosphates; D000110:Acetylcholinesterase