title: CD47 regulates the TGF-β signaling pathway in osteoblasts and is distributed in Meckel's cartilage.
abstract: Previously, CD47 gene expression has been shown to increase during mandible development using a micro array technique. To determine the function of CD47 in osteoblasts, CD47 was silenced using siRNA in vitro. The TGF-β1 and phosphorylated-Smad2 levels and transcription factor genes related to bone metabolism increased dose-dependently with CD47 silencing. Furthermore, we determined the distribution of CD47 in mouse embryonic E13 and E15 in vivo. The CD47-positive cells were localized in Meckel's cartilage and antenatal mandibular bone. These results suggest that TGF-β1 signaling and mandible development might be regulated by CD47.
authors.affiliation: Department of Periodontology, Nihon University School of Dentistry, Tokyo, Japan. shimada-ki@dent.nihon-u.ac.jp
authors.forename: Koichi
authors.initials: K
authors.lastname: Shimada
authors.forename: Akira
authors.initials: A
authors.lastname: Nakajima
authors.forename: Kyoko
authors.initials: K
authors.lastname: Ikeda
authors.forename: Kazuya
authors.initials: K
authors.lastname: Ishibashi
authors.forename: Noriyoshi
authors.initials: N
authors.lastname: Shimizu
authors.forename: Koichi
authors.initials: K
authors.lastname: Ito
mesh_terms: D016475:3T3 Cells; D000818:Animals; D015153:Blotting, Western; D051928:CD47 Antigen / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D002356:Cartilage / Q000196:embryology*; D018929:Cell Culture Techniques; D004591:Electrophoresis, Polyacrylamide Gel; D020868:Gene Silencing / Q000502:physiology; D005865:Gestational Age; D007150:Immunohistochemistry; D008334:Mandible / Q000196:embryology* / Q000378:metabolism; D008648:Mesoderm / Q000502:physiology*; D051379:Mice; D010006:Osteoblasts / Q000502:physiology*; D016133:Polymerase Chain Reaction; D034741:RNA, Small Interfering / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology*; D051899:Smad2 Protein / Q000032:analysis / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics; D053773:Transforming Growth Factor beta1 / Q000032:analysis / Q000235:genetics / Q000502:physiology*
chemical_list: D051928:CD47 Antigen; C497895:Cd47 protein, mouse; D034741:RNA, Small Interfering; D051899:Smad2 Protein; C497783:Smad2 protein, mouse; D014157:Transcription Factors; D053773:Transforming Growth Factor beta1