title: Antimicrobial proline-rich peptides from the hemolymph of marine snail Rapana venosa.
abstract: Hemolymph of Rapana venosa snails is a complex mixture of biochemically and pharmacologically active components such as peptides and proteins. Antimicrobial peptides are gaining attention as antimicrobial alternatives to chemical food preservatives and commonly used antibiotics. Therefore, for the first time we have explored the isolation, identification and characterisation of 11 novel antimicrobial peptides produced by the hemolymph of molluscs. The isolated peptides from the hemolymph applying ultrafiltration and reverse-phase high-performance liquid chromatography (RP-HPLC) have molecular weights between 3000 and 9500 Da, determined by mass spectrometric analysis. The N-terminal sequences of the peptides identified by Edman degradation matched no peptides in the MASCOT search database, indicating novel proline-rich peptides. UV spectra revealed that these substances possessed the characteristics of protein peptides with acidic isoelectric points. However, no Cotton effects were observed between 190 and 280 nm by circular dichroism spectroscopy. Four of the pro-rich peptides also showed strong antimicrobial activities against tested microorganisms including Gram-positive and Gram-negative bacteria.
authors.affiliation: Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, G. Bonchev 9, Sofia 1113, Bulgaria. pda54@abv.bg
authors.forename: Pavlina
authors.initials: P
authors.lastname: Dolashka
authors.forename: Vesela
authors.initials: V
authors.lastname: Moshtanska
authors.forename: Valika
authors.initials: V
authors.lastname: Borisova
authors.forename: Aleksander
authors.initials: A
authors.lastname: Dolashki
authors.forename: Stefan
authors.initials: S
authors.lastname: Stevanovic
authors.forename: Tzvetan
authors.initials: T
authors.lastname: Dimanov
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Voelter
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D056148:Chromatography, Reverse-Phase; D002942:Circular Dichroism; D030562:Databases, Protein; D006090:Gram-Negative Bacteria / Q000187:drug effects* / Q000254:growth & development; D006094:Gram-Positive Bacteria / Q000187:drug effects* / Q000254:growth & development; D006458:Hemolymph / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011392:Proline / Q000737:chemistry / Q000378:metabolism; D012908:Snails / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D011392:Proline