title: Yeast surface display of lamprey variable lymphocyte receptors.
abstract: The variable lymphocyte receptors (VLRs) of lamprey and hagfish comprise leucine-rich repeat modules, instead of the immunoglobulin-like domain building blocks of antibodies and T-cell receptors in jawed vertebrates. Both types of vertebrate-rearranging antigen receptors are similarly diverse, with repertoires that can potentially exceed 10(14) unique receptors. In order to characterize antigen-binding properties of the VLRs, we developed a high-throughput yeast surface display platform for the isolation of monoclonal VLRs. We have isolated VLRs that specifically bind hen egg lysozyme, β-galactosidase, cholera toxin subunit B, R-phycoerythrin, and the blood group trisaccharides A and B, with binding affinities in the mid-nanomolar to mid-picomolar range. VLRs may, thus, be excellent single-chain alternatives to Ig-based antibodies for biotechnology applications.
authors.affiliation: Center of Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, MD, USA.
authors.forename: Gang
authors.initials: G
authors.lastname: Xu
authors.forename: Satoshi
authors.initials: S
authors.lastname: Tasumi
authors.forename: Zeev
authors.initials: Z
authors.lastname: Pancer
mesh_terms: D000818:Animals; D002772:Cholera Toxin / Q000378:metabolism; D007798:Lampreys / Q000235:genetics / Q000378:metabolism*; D009113:Muramidase / Q000378:metabolism; D011485:Protein Binding; D011946:Receptors, Antigen / Q000235:genetics / Q000378:metabolism*; D015003:Yeasts / Q000235:genetics / Q000378:metabolism*; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D011946:Receptors, Antigen; D002772:Cholera Toxin; C421864:hen egg lysozyme; D009113:Muramidase; D001616:beta-Galactosidase