title: [Studies on identification and secondary metabolites of endophytic fungi strain E8 from Curcuma wenyujin].
abstract: OBJECTIVE
  To identify the endophyte strain E8 with high activity from Curcuma wenyujin and study its secondary metabolites.
  
  
  METHODS
  The strain E8 was identified by morphological observation and ITS sequence analysis. Manifold chromatographic methods were used to separate and purify the chemical constituents of fermentation broth from strain E8, and their structures were identified by physiochemical properties and spectral data.
  
  
  RESULTS
  The strain E8 belongs to P. oxalicum. Four compounds were isolated from the fermentation broth of this strain and elucidated as chrysophanol, emodin, secalonic acid A and beta-sitosterol.
  
  
  CONCLUSIONS
  The endophyte P. oxalicum was isolated from medicinal plant Curcuma wenyujin for the first time. Four compounds were first isolated from endophytic fungus in C. wenyujin. Thus, microbial fermentation is a new access for these compounds production.
authors.affiliation: Jilin Agriculture University, Ginseng and Cartialgenous Testing Center of Ministry of Agriculture of the People's Republic of China, Changchun 130118, China. yanhong-w@163.com
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Wu
authors.forename: Xindong
authors.initials: X
authors.lastname: Yang
authors.forename: Xiaokun
authors.initials: X
authors.lastname: Li
mesh_terms: D000880:Anthraquinones / Q000032:analysis; D030024:Curcuma / Q000382:microbiology*; D004642:Emodin / Q000032:analysis; D005285:Fermentation; D010407:Penicillium / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D012855:Sitosterols / Q000032:analysis; D044004:Xanthones / Q000032:analysis
chemical_list: D000880:Anthraquinones; D012855:Sitosterols; D044004:Xanthones; C009218:secalonic acid; C025473:gamma-sitosterol; D004642:Emodin; C027113:chrysophanic acid