title: Establishment of a pair of novel cloned tumour cell lines with or without metastatic potential from canine mammary adenocarcinoma.
abstract: We produced 23 cloned cell lines from parental CHMp, which was previously established from a canine mammary adenocarcinoma patient in our laboratory. Two representative cloned cell lines, namely, CHMp-5b and -13a, were selected and characterized for cellular morphology, growth potential and expression of some tumour-related proteins. Subsequently, we transplanted the 2 tumour cell lines orthotopically into female nude mice to examine their tumorigenicity and metastatic potential. Interestingly, despite sharing the same origin, only CHMp-5b cells metastasized to the lung. Our results indicate that a comparison between these 2 cell lines at the molecular level will help us understand mechanisms of tumour progression, especially in the context of distant metastases originating from canine mammary gland tumours.
authors.affiliation: Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Murai
authors.forename: T
authors.initials: T
authors.lastname: Nakagawa
authors.forename: Y
authors.initials: Y
authors.lastname: Endo
authors.forename: A
authors.initials: A
authors.lastname: Kamida
authors.forename: K
authors.initials: K
authors.lastname: Yoshida
authors.forename: M
authors.initials: M
authors.lastname: Mochizuki
authors.forename: R
authors.initials: R
authors.lastname: Nishimura
authors.forename: N
authors.initials: N
authors.lastname: Sasaki
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology / Q000503:physiopathology / Q000662:veterinary*; D000818:Animals; D015153:Blotting, Western / Q000662:veterinary; D045744:Cell Line, Tumor / Q000502:physiology*; D004285:Dogs; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D008175:Lung Neoplasms / Q000556:secondary; D015674:Mammary Neoplasms, Animal / Q000473:pathology / Q000503:physiopathology*; D051379:Mice; D008819:Mice, Nude; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation / Q000662:veterinary