title: Chemoenzymatic synthesis and hydrogelation of amylose-grafted xanthan gums.
abstract: This paper reports the chemoenzymatic synthesis of an amylose-grafted xanthan gum. An amine-functionalized maltooligosaccharide was chemically introduced to xanthan gum by condensation with its carboxylates using a condensing agent to produce a maltooligosaccharide-grafted xanthan gum. Then, a phosphorylase-catalyzed enzymatic polymerization of glucose 1-phosphate from the graft chain ends on the xanthan gum derivative was performed, giving an amylose-grafted xanthan gum. Furthermore, the product formed a gel with an ionic liquid, which was converted into a hydrogel with high water content by replacement of the ionic liquid with water. The ionically cross-linked hydrogel was also provided by soaking the primary formed hydrogel in FeCl(3) aqueous solution. The mechanical properties of the resulting hydrogels were evaluated by compressive testing.
authors.affiliation: Graduate School of Science and Engineering, Kagoshima University, Kagoshima, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Arimura
authors.forename: Yuji
authors.initials: Y
authors.lastname: Omagari
authors.forename: Kazuya
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Kadokawa
mesh_terms: D000688:Amylose / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry; D017666:Deuterium Oxide / Q000737:chemistry; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D052578:Ionic Liquids / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D009844:Oligosaccharides / Q000737:chemistry; D058105:Polymerization; D011135:Polysaccharides, Bacterial / Q000138:chemical synthesis* / Q000737:chemistry; D014961:X-Ray Diffraction
chemical_list: D003432:Cross-Linking Reagents; D052578:Ionic Liquids; D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial; C021705:maltooligosaccharides; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D000688:Amylose; D017666:Deuterium Oxide; C002563:xanthan gum