title: [Distribution and elimination of thiabendazole and its metabolite residue in laying hens].
abstract: OBJECTIVE
  To explore the distribution and elimination of thiabendazole and its metabolite 5-hydrothiabendazole residues in the hens tissues including liver, muscle, heart, fat, as well as in eggs.
  
  
  METHODS
  Laying hens were orally administred thiabendazole for 5 consecutive days (100mg per hen daily) and then the hens were sacrificed at the times of 1 day, 3 days, 5 days and 7 days after the end of treatment. Eggs, liver,muscle,fat and heart tissues were collected and homogenized. The samples were extracted by acetonitrile, further concentrated and purified by an Oasis MCX cartridge, and then the contents of thiabendazole and 5-hydrothiabendazole in tissue homogenates determinated by liquid chromatography electrospray ionisation tandem mass spectrometry (UPLC-ESI-MS/MS).
  
  
  RESULTS
  The major residues in the tissue homogenates was 5-hydroxythiabendazole, with more higher concentrations than thiabendazole. Egg samples presented the large majority of both drug residues. For the tissue homogenates,the total concentrations of thiabendazole and 5-hydrothiabendazole residues followed the order of liver > heart > muscle > fat at 1 day after the treatment. The withdrawal period of thiabendazole for eggs was about 7 days.
  
  
  CONCLUSIONS
  Distribution and elimination of thiabendazole and its metabolite residues in laying hens were primarily studied in this study.
authors.affiliation: Shanxi Medical University, Taiyuan, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Shang
authors.forename: Juan
authors.initials: J
authors.lastname: Guo
authors.forename: Yin
authors.initials: Y
authors.lastname: Zhang
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Qingshan
authors.initials: Q
authors.lastname: Li
authors.forename: Bing
authors.initials: B
authors.lastname: Shao
mesh_terms: D000818:Animals; D000969:Antinematodal Agents / Q000008:administration & dosage / Q000493:pharmacokinetics*; D002645:Chickens; D004350:Drug Residues / Q000493:pharmacokinetics; D013827:Thiabendazole / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000378:metabolism / Q000493:pharmacokinetics*; D014018:Tissue Distribution
chemical_list: D000969:Antinematodal Agents; C035151:5-hydroxythiabendazole; D013827:Thiabendazole