title: 5'-(N-ethylcarboxamido) adenosine improves angiogenesis in transplanted human ovarian tissue.
abstract: To investigate the effect of 5'-(N-ethylcarboxamido) adenosine (NECA), an adenosine agonist, on triggering angiogenesis in transplanted human ovarian tissue, the expression of angiopoietin-1 (Ang1), Ang2, vascular endothelial growth factor 121 (VEGF-121) and VEGF-189 at both gene and protein levels as well as the density of vasculature were studied in tissue fragments grafted to NECA-treated and control groups of nude mice. The results showed that NECA treatment triggered down-regulation of Ang1, induced VEGF-189 expression, and stimulated neovascularization, highlighting the beneficial effect of NECA on the process of angiogenesis.
authors.affiliation: Biochemistry Department, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Hormozi
authors.forename: Saeed
authors.initials: S
authors.lastname: Talebi
authors.forename: Amir Hassan
authors.initials: AH
authors.lastname: Zarnani
authors.forename: Mahmood
authors.initials: M
authors.lastname: Jeddi-Tehrani
authors.forename: Ladan Hosseini
authors.initials: LH
authors.lastname: Gohari
authors.forename: Haleh
authors.initials: H
authors.lastname: Soltanghoraei
authors.forename: Mina
authors.initials: M
authors.lastname: Jafarabadi
authors.forename: Mohammad Mehdi
authors.initials: MM
authors.lastname: Akhondi
mesh_terms: D019830:Adenosine-5'-(N-ethylcarboxamide) / Q000494:pharmacology*; D000328:Adult; D043925:Angiogenesis Inducing Agents / Q000494:pharmacology*; D042683:Angiopoietin-1 / Q000235:genetics / Q000378:metabolism; D042702:Angiopoietin-2 / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001706:Biopsy; D005260:Female; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D010053:Ovary / Q000098:blood supply / Q000187:drug effects* / Q000378:metabolism / Q000637:transplantation*; D012333:RNA, Messenger / Q000378:metabolism; D013997:Time Factors; D014183:Transplantation, Heterologous; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism
chemical_list: C467630:ANGPT1 protein, human; D043925:Angiogenesis Inducing Agents; D042683:Angiopoietin-1; D042702:Angiopoietin-2; D012333:RNA, Messenger; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D019830:Adenosine-5'-(N-ethylcarboxamide)