title: The relationship between fat depot-specific preadipocyte differentiation and metabolic syndrome in obese women.
abstract: OBJECTIVE
  Obesity is strongly associated with metabolic syndrome, but not all obese individuals display a clustering of metabolic risk factors. Recent studies have shown that in vitro subcutaneous (SC)-preadipocyte differentiation is negatively associated with obesity. These results suggest that impaired adipogenesis is an important factor linking obesity to metabolic disorders. We examined whether in vitro preadipocyte differentiation is associated with metabolic syndrome, independent of obesity.
  
  
  METHODS
  Paired adipose tissue samples were obtained from the 13 nonobese women and the 65 obese women. The CD34(+)/CD31(-) cells were isolated from the stromal-vascular fraction of both SC and omental (OM) fat depots by immune magnetic separation, and the subset was cultured with a differentiation cocktail. Then, we analysed the relationship between the degree of preadipocyte differentiation and metabolic factors.
  
  
  RESULTS
  Obese women without metabolic syndrome (n = 37) had significantly higher SC-preadipocyte differentiation than equally obese women with metabolic syndrome (n = 28); however, OM-preadipocyte differentiation was similar in both groups. SC-preadipocyte differentiation was strongly correlated with triglycerides, HDL cholesterol, homoeostasis model assessment of insulin resistance and OM-adipocyte size. However, OM-preadipocyte differentiation was not correlated with any of these parameters.
  
  
  CONCLUSIONS
  This study identified that SC-preadipocyte differentiation is associated with metabolic syndrome independent of obesity, whereas OM-preadipocyte differentiation is not. These findings suggest that, in the setting of obesity, an enhanced adipogenic capacity of SC depots could be protective for metabolic syndrome. Our data underscores an interaction between adipose tissue homoeostasis and metabolic disorder.
authors.affiliation: Department of Obstetrics and Gynecology, Korea University College of Medicine, Seoul, Korea.
authors.forename: Hyun Tae
authors.initials: HT
authors.lastname: Park
authors.forename: Eun Sil
authors.initials: ES
authors.lastname: Lee
authors.forename: Yong-Pil
authors.initials: YP
authors.lastname: Cheon
authors.forename: Dong Ryul
authors.initials: DR
authors.lastname: Lee
authors.forename: Kyung-Sook
authors.initials: KS
authors.lastname: Yang
authors.forename: Young Tae
authors.initials: YT
authors.lastname: Kim
authors.forename: Jun Young
authors.initials: JY
authors.lastname: Hur
authors.forename: Sun Haeng
authors.initials: SH
authors.lastname: Kim
authors.forename: Kyu Wan
authors.initials: KW
authors.lastname: Lee
authors.forename: Tak
authors.initials: T
authors.lastname: Kim
mesh_terms: D017667:Adipocytes / Q000166:cytology* / Q000502:physiology*; D000328:Adult; D000368:Aged; D050218:Body Fat Distribution; D002454:Cell Differentiation; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D050152:Intra-Abdominal Fat / Q000166:cytology / Q000378:metabolism; D016131:Lymphocyte Subsets; D024821:Metabolic Syndrome / Q000378:metabolism*; D008875:Middle Aged; D009765:Obesity / Q000378:metabolism*; D050151:Subcutaneous Fat / Q000166:cytology / Q000378:metabolism