title: Antitussive and immunomodulating activities of instant coffee arabinogalactan-protein.
abstract: A low molecular mass arabinogalactan-protein (AGP) composed of galactose and arabinose with a low protein content, isolated from the instant coffee powder of Coffea arabica beans, has been tested on antitussive (in vivo) and immunomodulating (ex vivo) activities. The results of antitussive tests revealed a significant dose dependant cough-suppressive effect of coffee AGP. It was observed 30 or 60 min after AGP administration and its efficacy lasted during the entire experiment course. Immunological tests showed that AGP affected some mediators of immunocompetent cells of immune system as TNF-α, IFN-γ and IL-2 cytokines. It seems that coffee AGP is a good inductor of both pro-inflammatory cytokines TNF-α and IFN-γ, however, less potent in TNF-α induction in comparison with that of β-D-glucan. Evident induction of TNF-α, IL-2 and IFN-γ cytokines, pro-TH1 polarization supports our conclusion about bio-immunological efficacy of AGP with an emphasis on the cellular immunity.
authors.affiliation: Department of Pharmacology, Jessenius Faculty of Medicine, Comenius University, Martin, Slovakia.
authors.forename: G
authors.initials: G
authors.lastname: Nosáľová
authors.forename: L
authors.initials: L
authors.lastname: Prisenžňáková
authors.forename: E
authors.initials: E
authors.lastname: Paulovičová
authors.forename: P
authors.initials: P
authors.lastname: Capek
authors.forename: M
authors.initials: M
authors.lastname: Matulová
authors.forename: L
authors.initials: L
authors.lastname: Navarini
authors.forename: F Suggi
authors.initials: FS
authors.lastname: Liverani
mesh_terms: D000284:Administration, Oral; D000403:Airway Resistance / Q000187:drug effects; D000818:Animals; D000996:Antitussive Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D003069:Coffee / Q000737:chemistry*; D003371:Cough / Q000188:drug therapy / Q000503:physiopathology; D006168:Guinea Pigs; D007155:Immunologic Factors / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D007376:Interleukin-2 / Q000378:metabolism; D051379:Mice; D009088:Mucoproteins / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D010940:Plant Proteins / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D011208:Powders; D013154:Spleen / Q000187:drug effects / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000996:Antitussive Agents; D003069:Coffee; D007155:Immunologic Factors; D007376:Interleukin-2; D009088:Mucoproteins; D010940:Plant Proteins; D011208:Powders; D014409:Tumor Necrosis Factor-alpha; C006619:arabinogalactan proteins