title: A proteomic study of TAR-RNA binding protein (TRBP)-associated factors.
abstract: BACKGROUND
  The human TAR RNA-binding protein, TRBP, was first identified and cloned based on its high affinity binding to the small hairpin trans-activation responsive (TAR) RNA of HIV-1. TRBP has more recently been found to be a constituent of the RNA-induced silencing complex (RISC) serving as a Dicer co-factor in the processing of the ~70 nucleotide pre-microRNAs(miRNAs) to 21-25 nucleotide mature miRNAs.
  
  
  RESULTS
  Using co-immunoprecipitation and protein-identification by mass spectrometry, we characterized intracellular proteins that complex with TRBP. These interacting proteins include those that have been described to act in protein synthesis, RNA modifications and processing, DNA transcription, and cell proliferation.
  
  
  CONCLUSIONS
  Our findings provide a proteome of factors that may cooperate with TRBP in activities such as miRNA processing and in RNA interference by the RISC complex.
authors.affiliation: Molecular Virology Section, Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA. kjeang@niaid.nih.gov.
authors.forename: Ya-Hui
authors.initials: YH
authors.lastname: Chi
authors.forename: Oliver John
authors.initials: OJ
authors.lastname: Semmes
authors.forename: Kuan-Teh
authors.initials: KT
authors.lastname: Jeang