title: Development of a highly sensitive fluorescence probe for hydrogen peroxide.
abstract: Hydrogen peroxide is believed to play a role in cellular signal transduction by reversible oxidation of proteins. Here, we report the design and synthesis of a novel fluorescence probe for hydrogen peroxide, utilizing a photoinduced electron transfer strategy based on benzil chemistry to control the fluorescence. The practical value of this highly sensitive and selective fluorescence probe, NBzF, was confirmed by its application to imaging of hydrogen peroxide generation in live RAW 264.7 macrophages. NBzF was also employed for live cell imaging of hydrogen peroxide generated as a signaling molecule in A431 human epidermoid carcinoma cells.
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Abo
authors.forename: Yasuteru
authors.initials: Y
authors.lastname: Urano
authors.forename: Kenjiro
authors.initials: K
authors.lastname: Hanaoka
authors.forename: Takuya
authors.initials: T
authors.lastname: Terai
authors.forename: Toru
authors.initials: T
authors.lastname: Komatsu
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Nagano
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004579:Electron Transport; D005452:Fluoresceins / Q000032:analysis* / Q000737:chemistry*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis*; D008264:Macrophages / Q000737:chemistry; D051379:Mice
chemical_list: C561962:5-benzoylcarbonylfluorescein; D005452:Fluoresceins; D005456:Fluorescent Dyes; D006861:Hydrogen Peroxide