title: Culture-independent multi-locus variable-number tandem-repeat analysis (MLVA) of Mycoplasma pneumoniae.
abstract: A PCR approach for multi-locus variable-number tandem-repeat analysis (MLVA) of Mycoplasma pneumoniae directly from respiratory samples was developed and evaluated on 54 specimens from M. pneumoniae-positive pneumonia patients. The method resulted in a clearly identifiable MLVA type in all samples tested and can be used for MLVA without laborious isolation of the pathogen.
authors.affiliation: Dresden University of Technology, Medical Faculty Carl Gustav Carus, Institute of Medical Microbiology and Hygiene, Fetscherstrasse 74, D, 01307 Dresden, Germany. roger.dumke@tu-dresden.de
authors.forename: Roger
authors.initials: R
authors.lastname: Dumke
authors.forename: Enno
authors.initials: E
authors.lastname: Jacobs
mesh_terms: D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D006801:Humans; D018598:Minisatellite Repeats / Q000235:genetics*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D058889:Molecular Typing / Q000379:methods; D009177:Mycoplasma pneumoniae / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism; D011019:Pneumonia, Mycoplasma / Q000175:diagnosis; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D004269:DNA, Bacterial