title: Streamlined process for the chemical synthesis of RNA using 2'-O-thionocarbamate-protected nucleoside phosphoramidites in the solid phase.
abstract: An improved method for the chemical synthesis of RNA was developed utilizing a streamlined method for the preparation of phosphoramidite monomers and a single-step deprotection of the resulting oligoribonucleotide product using 1,2-diamines under anhydrous conditions. The process is compatible with most standard heterobase protection and employs a 2'-O-(1,1-dioxo-1λ(6)-thiomorpholine-4-carbothioate) as a unique 2'-hydroxyl protective group. Using this approach, it was demonstrated that the chemical synthesis of RNA can be as simple and robust as the chemical synthesis of DNA.
authors.affiliation: Agilent Laboratories, Agilent Technologies, Inc., 5301 Stevens Creek Boulevard, Santa Clara, California 95051, USA. doug_dellinger@agilent.com
authors.forename: Douglas J
authors.initials: DJ
authors.lastname: Dellinger
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Timár
authors.forename: Joel
authors.initials: J
authors.lastname: Myerson
authors.forename: Agnieszka B
authors.initials: AB
authors.lastname: Sierzchala
authors.forename: John
authors.initials: J
authors.lastname: Turner
authors.forename: Fernando
authors.initials: F
authors.lastname: Ferreira
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Kupihár
authors.forename: Geraldine
authors.initials: G
authors.lastname: Dellinger
authors.forename: Kenneth W
authors.initials: KW
authors.lastname: Hill
authors.forename: James A
authors.initials: JA
authors.lastname: Powell
authors.forename: Jeffrey R
authors.initials: JR
authors.lastname: Sampson
authors.forename: Marvin H
authors.initials: MH
authors.lastname: Caruthers
mesh_terms: D015394:Molecular Structure; D009025:Morpholines / Q000737:chemistry*; D009705:Nucleosides / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000737:chemistry*; D012313:RNA / Q000138:chemical synthesis* / Q000737:chemistry; D013457:Sulfur Compounds / Q000737:chemistry*
chemical_list: C568179:2'-O-(1,1-dioxo-1lamda(6)-thiomorpholine-4-carbothioate); D009025:Morpholines; D009705:Nucleosides; D009943:Organophosphorus Compounds; D013457:Sulfur Compounds; C434331:phosphoramidite; D012313:RNA