title: Proteomic analysis of a spring wheat cultivar in response to prolonged cold stress.
abstract: Cold represents one of the major abiotic factors influencing plant growth and development worldwide. We analysed the long-term responsiveness of an Iranian spring wheat (cv. Kohdasht) to cold from a proteomic point of view, in order to unravel the molecular mechanisms helping a cold-sensitive cultivar to survive exposure to suboptimal temperatures. Plants were grown at 20 or 4°C until entering the reproductive stage and a cross-comparison on the leaf proteomes was performed. Quantitative analyses on protein alterations occurring upon low-temperature exposure showed a reinforcement in ascorbate recycling (dehydroascorbate reductase, ascorbate peroxidase) and protein processing (proteasome subunit, cysteine proteinase), as well as the accumulation of the enzyme devoted to tetrapyrrole resynthesis (glutamate semialdehyde aminomutase). In contrast, among proteins down-regulated after cold stress, we could identify some key Krebs cycle enzymes (isocitrate dehydrogenase, malate dehydrogenase), together with many photosynthesis-related proteins (oxygen-evolving complex proteins, ATP synthase subunits, ferredoxin NADPH oxidoreductase and some Calvin cycle enzymes). Physiological and biochemical parameters (such as shoot apex dissection, chlorophyll, proline and sugar content determination) sustained proteomics findings allowing the present research to contribute to the current knowledge on these long-term responses, which may be crucial to stress adaptation under field conditions.
authors.affiliation: Department of Environmental Sciences, University of Tuscia, Largo dell'Università snc, Viterbo, Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: Rinalducci
authors.forename: Maria Giulia
authors.initials: MG
authors.lastname: Egidi
authors.forename: Ghasem
authors.initials: G
authors.lastname: Karimzadeh
authors.forename: Ferdous Rastgar
authors.initials: FR
authors.lastname: Jazii
authors.forename: Lello
authors.initials: L
authors.lastname: Zolla
mesh_terms: D003080:Cold Temperature; D058639:Cold-Shock Response / Q000502:physiology*; D015180:Electrophoresis, Gel, Two-Dimensional; D010641:Phenotype; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism; D010940:Plant Proteins / Q000032:analysis* / Q000145:classification / Q000378:metabolism; D020543:Proteome / Q000032:analysis* / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D012333:RNA, Messenger / Q000032:analysis; D018749:RNA, Plant / Q000032:analysis; D017382:Reactive Oxygen Species; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012273:Ribulose-Bisphosphate Carboxylase; D014908:Triticum / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D020543:Proteome; D012333:RNA, Messenger; D018749:RNA, Plant; D017382:Reactive Oxygen Species; D012273:Ribulose-Bisphosphate Carboxylase