title: A comparative study of induced pluripotent stem cells generated from frozen, stocked bone marrow- and adipose tissue-derived mesenchymal stem cells.
abstract: Bone marrow-derived mesenchymal stem cells (BMSCs) and adipose tissue-derived mesenchymal stem cells (AMSCs) have been used clinically for tissue regeneration; however, their proliferation/differentiation potentials are limited. Recently, induced pluripotent stem cells (iPSCs), known to have nearly unlimited potential to proliferate and differentiate into cells of all three germ layers, have gained wide interest in regenerative medicine. Here, we generated iPSCs from frozen-stocked AMSCs and BMSCs and examined their biological characteristics by comparative analyses. Although the iPSCs were more challenging to generate from the BMSCs than the AMSCs, both iPSC populations expressed pluripotent markers, such as stage-specific embryonic antigen (SSEA)-3, SSEA-4, tumour-related antigens (TRAs) TRA-1-60 and TRA-1-81, OCT3/4 and NANOG. Furthermore, both cell populations differentiated well into three germ layer-derived cells, both in vitro and in vivo. These results indicate that iPSCs derived from frozen AMSCs/BMSCs exhibit equally acceptable iPSC characteristics and have potential in clinical applications as an alternative source of autogenous stem cells.
authors.affiliation: Tissue Engineering Research Group, Health Research Institute, National Institute of Advanced Industrial Science and Technology, Amagasaki site, Amagasaki, Hyogo, Japan.
authors.forename: Hiroe
authors.initials: H
authors.lastname: Ohnishi
authors.forename: Yasuaki
authors.initials: Y
authors.lastname: Oda
authors.forename: Tetsuhiro
authors.initials: T
authors.lastname: Aoki
authors.forename: Mika
authors.initials: M
authors.lastname: Tadokoro
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Katsube
authors.forename: Hajime
authors.initials: H
authors.lastname: Ohgushi
authors.forename: Koji
authors.initials: K
authors.lastname: Hattori
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Yuba
mesh_terms: D050156:Adipogenesis; D000273:Adipose Tissue / Q000166:cytology*; D000954:Antigens, Surface / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology*; D002469:Cell Separation / Q000379:methods*; D048430:Cell Shape; D015925:Cryopreservation / Q000379:methods*; D058732:Embryoid Bodies / Q000166:cytology / Q000378:metabolism; D005434:Flow Cytometry; D005615:Freezing; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D007621:Karyotyping; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D010012:Osteogenesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013724:Teratoma / Q000473:pathology
chemical_list: D000954:Antigens, Surface