title: Isolation of bacterial extrachromosomal DNA from human dental plaque associated with periodontal disease, using transposon-aided capture (TRACA).
abstract: The human oral cavity is host to a complex microbial community estimated to comprise >700 bacterial species, of which at least half are thought to be not yet cultivable in vitro. To investigate the plasmids present in this community, we used a transposon-aided capture system, which allowed the isolation of plasmids from human oral supra- and subgingival plaque samples. Thirty-two novel plasmids and a circular molecule that could be an integrase-generated circular intermediate were isolated.
authors.affiliation: Research Department of Microbial Diseases, UCL Eastman Dental Institute, University College London, London, UK.
authors.forename: Philip J
authors.initials: PJ
authors.lastname: Warburton
authors.forename: Elaine
authors.initials: E
authors.lastname: Allan
authors.forename: Stephanie
authors.initials: S
authors.lastname: Hunter
authors.forename: John
authors.initials: J
authors.lastname: Ward
authors.forename: Veronica
authors.initials: V
authors.lastname: Booth
authors.forename: William G
authors.initials: WG
authors.lastname: Wade
authors.forename: Peter
authors.initials: P
authors.lastname: Mullany
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D004251:DNA Transposable Elements / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics / Q000302:isolation & purification; D003773:Dental Plaque / Q000382:microbiology*; D006801:Humans; D008969:Molecular Sequence Data; D010510:Periodontal Diseases / Q000235:genetics / Q000382:microbiology*; D010957:Plasmids / Q000235:genetics; D018626:Retroelements
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D018626:Retroelements