title: Laser scanning cytometry of mitosis: state and stage analysis.
abstract: Here we use a concept of cell state, which can be defined as the conjunction of expression levels of an arbitrary number of biomolecules or modifications thereof that oscillate, to classify mitotic cells. We describe detection of cell states with quantitative immunofluorescence measurements performed by laser scanning cytometry. This platform allows both measurement of the cell states, capture of cell images within those states, and subsequent analysis of each image to classify by traditional mitotic stages based on nuclear morphology.
authors.affiliation: Case Comprehensive Cancer Center, Case Western Reserve University, Cleveland, Ohio, USA.
authors.forename: Tammy
authors.initials: T
authors.lastname: Stefan
authors.forename: James W
authors.initials: JW
authors.lastname: Jacobberger
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D002461:Cell Line, Transformed; D056751:Cyclin A2 / Q000378:metabolism; D056744:Cyclin B1 / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D042783:Endothelial Cells / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism; D006801:Humans; D047569:Laser Scanning Cytometry / Q000379:methods*; D008938:Mitosis; D013194:Staining and Labeling / Q000379:methods
chemical_list: D015415:Biomarkers; C503721:CCNA2 protein, human; C534699:CCNB1 protein, human; D056751:Cyclin A2; D056744:Cyclin B1; D004247:DNA