title: Protection against genotoxic damages following whole body gamma radiation exposure in mice by lipoic acid.
abstract: Alpha-lipoic acid (LA) protected plasmid pBR 322 DNA, under in vitro conditions from gamma radiation induced strand breaks as evidenced by the prevention of the loss of supercoiled covalently closed circular form upon irradiation. It also protected the membrane lipids of liver homogenates from the oxidative damages. Whole body exposure of mice to gamma-radiation resulted in damage to cellular DNA in various tissues and administration of LA prior to the radiation exposure prevented the radiation induced DNA damage as assessed by alkaline comet assay. Administration of LA to mice prior to the radiation exposure also prevented induction of chromosomal damages in bone marrow cells and formation of micronuclei in blood reticulocytes. Thus taken together, LA a normal cellular constituent could be used as a radioprotector against whole body radiation exposure scenarios.
authors.affiliation: Amala Cancer Research Centre, Thrissur 680555, Kerala, India.
authors.forename: Lakshmy
authors.initials: L
authors.lastname: Ramachandran
authors.forename: Cherupally Krishnan Krishnan
authors.initials: CK
authors.lastname: Nair
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000528:radiation effects; D002869:Chromosome Aberrations / Q000187:drug effects; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects* / Q000528:radiation effects*; D005720:Gamma Rays; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects / Q000528:radiation effects; D008297:Male; D051379:Mice; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects; D011835:Radiation Protection / Q000379:methods; D011837:Radiation-Protective Agents / Q000494:pharmacology; D008063:Thioctic Acid / Q000494:pharmacology*; D014916:Whole-Body Irradiation / Q000401:mortality
chemical_list: D011837:Radiation-Protective Agents; D008063:Thioctic Acid