title: [Rapid method study of methicillin-resistant Staphylococcus aureus identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry].
abstract: OBJECTIVE
  To establish the method on rapid identification of Staphylococcus aureus and the performance of methicillin-resistance.
  
  
  METHODS
  40 isolation strains of staphylococcal were analyzed by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF-MS) and API Staph Ident system, and the performance of methicillin-resistance was tested by Disk Diffusion Susceptibility Testing.
  
  
  RESULTS
  34 isolation strains were identified by MALDI-TOF-MS as Staphylococcus aureus, and the result was uniform to the API Staph Ident system. 11 isolation strains were identified as methicillin-resistant Staphylococcus aureus (MRSA) and 23 isolation strains were methicillin-susceptible to Staphylococcus aureus (MSSA). All the 34 isolation strains were clustered to 2 species identified by MALDI-TOF-MS. The result was uniform to the Disk Diffusion Susceptibility Testing.
  
  
  CONCLUSIONS
  These results demonstrated that MALDI-TOF-MS could be a rapid technique for identification and discrimination of MRSA.
authors.affiliation: Institute for Environment Hygiene and Health Related Product Safety, Chinese Center for Disease 100021, China. Control and Prevent, Beijing, China. sunzongke@yahoo.com.cn
authors.forename: Zongke
authors.initials: Z
authors.lastname: Sun
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Xiping
authors.initials: X
authors.lastname: Chen
mesh_terms: D055624:Methicillin-Resistant Staphylococcus aureus / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013203:Staphylococcal Infections / Q000382:microbiology