title: The effect of the introduction of exogenous strain Acidithiobacillus thiooxidans A01 on functional gene expression, structure and function of indigenous consortium during pyrite bioleaching.
abstract: Acidithiobacillus thiooxidans A01 was added to a consortium of bioleaching bacteria including Acidithiobacilluscaldus, Leptospirillumferriphilum, Acidithiobacillus ferrooxidans, Sulfobacillus thermosulfidooxidans, Acidiphilium spp., and Ferroplasma thermophilum cultured in modified 9 K medium containing 0.5% (w/v) pyrite, and 10.7% increase of bioleaching rate was observed. Changes in community structure and gene expression were monitored with real-time PCR and functional gene arrays (FGAs). Real-time PCR showed that addition of At. thiooxidans caused increased numbers of all consortium members except At. caldus, and At. caldus, L. ferriphilum, and F. thermophilum remained dominant in this community. FGAs results showed that after addition of At. thiooxidans, most genes involved in iron, sulfur, carbon, and nitrogen metabolisms, metal resistance, electron transport, and extracellular polymeric substances of L. ferriphilum, F. thermophilum, and Acidiphilium spp., were up-regulated while most of these genes were down-regulated at 70-78 h in At. caldus and up-regulated in At. ferrooxidans, then down-regulated at 82-86 h.
authors.affiliation: School of Minerals Processing and Bioengineering, Central South University, Changsha, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.forename: Huaqun
authors.initials: H
authors.lastname: Yin
authors.forename: Weimin
authors.initials: W
authors.lastname: Zeng
authors.forename: Yili
authors.initials: Y
authors.lastname: Liang
authors.forename: Yao
authors.initials: Y
authors.lastname: Liu
authors.forename: Ngom
authors.initials: N
authors.lastname: Baba
authors.forename: Guanzhou
authors.initials: G
authors.lastname: Qiu
authors.forename: Li
authors.initials: L
authors.lastname: Shen
authors.forename: Xian
authors.initials: X
authors.lastname: Fu
authors.forename: Xueduan
authors.initials: X
authors.lastname: Liu
mesh_terms: D013856:Acidithiobacillus thiooxidans / Q000378:metabolism*; D001483:Base Sequence; D018441:Biofilms; D016000:Cluster Analysis; D003470:Culture Media; D017931:DNA Primers; D015870:Gene Expression; D007501:Iron / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D060888:Real-Time Polymerase Chain Reaction; D013440:Sulfides / Q000378:metabolism*
chemical_list: D003470:Culture Media; D017931:DNA Primers; D013440:Sulfides; C011342:pyrite; D007501:Iron