title: [Expression of aquaporin 3, 4, and 8 in colonic mucosa of rat models with slow transit constipation].
abstract: OBJECTIVE
  To investigate the expression of aquaporin 3, 4, and 8 in the colonic mucosa of rat models with slow transit constipation (STC).
  
  
  METHODS
  STC rat model was established by giving the rats the compound solution of diphenoxylate. Real time polymerase chain reaction (RT-PCR) was used to measure the expression of aquaporin mRNA in colonic mucosa of STC rat models (study group,n=16) and normal rats (control group,n=16). Gray scale ratio of aquaporin to β-action (internal reference) was used for quantification.
  
  
  RESULTS
  RT-PCR revealed that the mean gray scale ratios of aquaporin 3 in the proximal colon of the study group and control group were 0.344 and 0.602 (P<0.05), and were 0.419 and 0.509 in the distal colon (P>0.05), respectively. The mean gray scale ratios of aquaporin 4 in the proximal and the distal colon were 0.764 and 0.759 in the study group (P>0.05), and were 0.776 and 0.736 in the control group (P>0.05), respectively. However, there was no expression of aquaporin 8 in the proximal and the distal colon in either the study group or the control groups.
  
  
  CONCLUSIONS
  Expression of aquaporin 3 in the proximal colon of STC rat models is down-regulated, which regulates water absorption. There are no significant changes in the expressions of aquaporin 4 and 8.
authors.affiliation: Department of General Surgery, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhi
authors.forename: Wei-tang
authors.initials: WT
authors.lastname: Yuan
mesh_terms: D000818:Animals; D051400:Aquaporin 3 / Q000378:metabolism*; D051401:Aquaporin 4 / Q000378:metabolism*; D020346:Aquaporins / Q000378:metabolism*; D003106:Colon / Q000378:metabolism*; D003248:Constipation / Q000378:metabolism*; D004195:Disease Models, Animal; D005260:Female; D007413:Intestinal Mucosa / Q000378:metabolism; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: C496029:Aqp3 protein, rat; C496032:Aqp4 protein, rat; D051401:Aquaporin 4; D020346:Aquaporins; C108330:aquaporin 8; D051400:Aquaporin 3