title: Compromised cellular responses to DNA damage accelerate chronological aging by incurring cell wall fragility in Saccharomyces cerevisiae.
abstract: Elevated levels of reactive oxygen species (ROS) can attack almost all cell components including genomic DNA to induce many types of DNA damage. In this study, we used Saccharomyces cerevisiae with various mutations in a biological network supposed to prevent deleterious effects of endogenous ROS to test the effect of such a network on yeast chronological aging. Our results showed that cells with defects in cellular antioxidation, DNA repair and DNA damage checkpoints displayed a mutation rate higher than that of wild-type strain. Moreover, the chronological life span of most mutants as determined by colony formation was found to be shorter than that of wild-type cells, especially for the mutants defective in DNA replication and DNA damage checkpoints, although the observed cell number was almost the same for wild-type and mutant strains. The mutants were finally found to be more sensitive to SDS and lysing enzyme treatment, and that the degree of sensitivity was correlated with their chronological life span.
authors.affiliation: The State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, No. 27 Shanda South Road, Jinan, 250100, Shandong, People's Republic of China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Yu
authors.forename: Xian-En
authors.initials: XE
authors.lastname: Zhang
authors.forename: Guanjun
authors.initials: G
authors.lastname: Chen
authors.forename: Weifeng
authors.initials: W
authors.lastname: Liu
mesh_terms: D002172:Canavanine / Q000494:pharmacology; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002473:Cell Wall / Q000187:drug effects / Q000378:metabolism*; D015169:Colony Count, Microbial; D004249:DNA Damage; D004260:DNA Repair / Q000187:drug effects; D004261:DNA Replication / Q000187:drug effects; D004351:Drug Resistance / Q000187:drug effects; D005434:Flow Cytometry; D017353:Gene Deletion; D005800:Genes, Fungal / Q000235:genetics; D059645:Mutation Rate; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D013997:Time Factors
chemical_list: D002172:Canavanine