title: Generation and detection of levuglandins and isolevuglandins in vitro and in vivo.
abstract: Levuglandins (LGs) and isolevuglandins (isoLGs), formed by rearrangement of endoperoxide intermediates generated through the cyclooxygenase and free radical induced oxidation of polyunsaturated fatty acids (PUFAs), are extraordinarily reactive, forming covalent adducts incorporating protein lysyl ε-amino groups. Because they accumulate, these adducts provide a dosimeter of oxidative injury. This review provides an updated and comprehensive overview of the generation of LG/isoLG in vitro and in vivo and the detection methods for the adducts of LG/isoLG and biological molecules in vivo.
authors.affiliation: Reproductive Medicine Center, Zhongnan Hospital of Wuhan University, Wuhan 430071, China. mjiinag@yahoo.com.cn
authors.forename: Ming
authors.initials: M
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Tao
authors.initials: T
authors.lastname: Li
mesh_terms: D000818:Animals; D006801:Humans; D015394:Molecular Structure; D011458:Prostaglandins E / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D011458:Prostaglandins E; C047684:levuglandin E2