title: Glomerulitis during acute cellular rejection may be a surrogate marker of vasculitis in renal allografts--better index for diagnosis of vasculitis.
abstract: BACKGROUND
  The Banff criteria (from 2005 to 2009) use "T cell-mediated rejection" to indicate acute cellular rejection. Vasculitis in smaller arteries is an important diagnostic criterion for moderate and severe T cell-mediated rejection. The renal allograft endothelium is a significant target of inflammatory response-mediated tissue damage. Medium-size arteries (arcuate arteries) are mostly absent in routine allograft biopsies, so identification of vasculitis relies on its identification in small arteries (arterioles to interlobar arteries). Although inflammation in terminal vessels such as the glomerular capillaries has been previously recognized, their role in grading the rejection process is not well characterized. We therefore evaluated the expression of CD3-positive T lymphocytes and CD68-positive macrophages in glomeruli, small arteries, and arcuate arteries of nephrectomy specimens obtained from transplant and renal tumor patients.
  
  
  METHODS
  The study group included 21 renal explant subjects with nonreversible moderate to severe T cell-mediated rejection (IIa to III) and/or severe chronic changes. The control group comprised 17 individuals with nephrectomy for renal tumors. In each case, a large renal section from cortex to medulla was stained for CD3 and CD68 by immunohistochemical method. CD3-positive T lymphocytes and CD68-positive macrophages per balanced high-power field were counted in glomeruli, interlobar arteries, and arcuate arteries.
  
  
  RESULTS
  In control kidney sections, neither CD3-positive T lymphocytes nor CD68-positive macrophages were noted in glomeruli, interlobar arteries, or arcuate arteries. In the study group, 15/21 showed diffuse C4d positivity. Also in the study group, positive CD3 and CD68 counts in glomeruli were significantly correlated to both interlobar and arcuate artery counts by linear regression analysis.
  
  
  CONCLUSIONS
  We conclude that in renal allograft biopsies, T lymphocytes and macrophages in the glomeruli not only represent a separate entity, "transplant glomerulitis," but also may be a surrogate marker of vasculitis present in larger vascular beds. Comparable amounts of T cells and macrophages imply that "acute cellular rejection" may be a better terminology to reflect the true inflammatory status.
authors.affiliation: Department of Anatomic Pathology, William Beaumont Hospital, Royal Oak, Michigan, USA.
authors.forename: H S
authors.initials: HS
authors.lastname: Desai
authors.forename: R K
authors.initials: RK
authors.lastname: Parasuraman
authors.forename: D
authors.initials: D
authors.lastname: Samarpungavan
authors.forename: M T
authors.initials: MT
authors.lastname: Rooney
authors.forename: S R
authors.initials: SR
authors.lastname: Cohn
authors.forename: G H
authors.initials: GH
authors.lastname: Reddy
authors.forename: L L
authors.initials: LL
authors.lastname: Rocher
authors.forename: F
authors.initials: F
authors.lastname: Dumler
authors.forename: P L
authors.initials: PL
authors.lastname: Zhang
mesh_terms: D015415:Biomarkers / Q000032:analysis*; D005921:Glomerulonephritis / Q000209:etiology*; D006801:Humans; D014657:Vasculitis / Q000150:complications / Q000175:diagnosis*
chemical_list: D015415:Biomarkers