title: Mechanophysical stimulations of mucin secretion in cultures of nasal epithelial cells.
abstract: Nasal epithelial cells secret mucins and are exposed in vivo to airflow-induced mechanophysical stresses, including wall shear stress (WSS), temperature, and humidity. In this work, human nasal epithelial cells cultured under air-liquid interface conditions were subjected to fields of airflow-induced oscillatory WSS at different temperature and humidity conditions. Changes in mucin secretion due to WSS were measured and the role of the cytoskeleton in mucin secretion was explored. Mucin secretion significantly increased in response to WSS in a magnitude-dependent manner with respect to static cultures and independently of the airflow temperature and humidity. In static cultures, mucin secretion decreased at high humidity with or without elevation of the temperature with respect to cultures at a comfortable climate. In cultures exposed to WSS, mucin secretion increased at high temperature with respect to cultures at comfortable climate conditions. The polymerization of actin microfilaments was shown to increase mucin secretion under WSS, whereas the dynamics of microtubule polymerization did not affect secretion. In conclusion, the data in this study show that mucin secretion is sensitive to oscillatory WSS as well as high temperature and humidity conditions.
authors.affiliation: Department of Biomedical Engineering, Faculty of Engineering, Tel Aviv University, Tel Aviv, Israel. nuritet@eng.tau.ac.il
authors.forename: Nurit Even-Tzur
authors.initials: NE
authors.lastname: Davidovich
authors.forename: Yoel
authors.initials: Y
authors.lastname: Kloog
authors.forename: Michael
authors.initials: M
authors.lastname: Wolf
authors.forename: David
authors.initials: D
authors.lastname: Elad
mesh_terms: D008841:Actin Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D000328:Adult; D001696:Biomechanical Phenomena / Q000187:drug effects; D002478:Cells, Cultured; D003078:Colchicine / Q000494:pharmacology; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D009077:Mucins / Q000378:metabolism*; D009297:Nasal Mucosa / Q000166:cytology* / Q000378:metabolism*; D013314:Stress, Mechanical; D014404:Tubulin / Q000378:metabolism
chemical_list: D009077:Mucins; D014404:Tubulin; D003078:Colchicine