title: Taxonomic and functional prokaryote diversity in mildly arsenic-contaminated sediments.
abstract: Arsenic-resistant prokaryote diversity is far from being exhaustively explored. In this study, the arsenic-adapted prokaryotic community present in a moderately arsenic-contaminated site near Sainte-Marie-aux-Mines (France) was characterized, using metaproteomic and 16S rRNA-encoding gene amplification. High prokaryotic diversity was observed, with a majority of Proteobacteria, Acidobacteria and Bacteroidetes, and a large archaeal community comprising Euryarchaeaota and Thaumarchaeota. Metaproteomic analysis revealed that Proteobacteria, Planctomycetes and Cyanobacteria are among the active bacteria in this ecosystem. Taken together, these results highlight the unsuspected high diversity of the arsenic-adapted prokaryotic community, with some phyla never having been described in highly arsenic-exposed sites.
authors.affiliation: Laboratoire de Génétique Moléculaire, Génomique, Microbiologie, Département Microorganismes, Génomes, Environnement, UMR7156 Université de Strasbourg/CNRS, 28 rue Goethe, 67083 Strasbourg Cedex, France. David.halter@gem.u-strasbg.fr
authors.forename: David
authors.initials: D
authors.lastname: Halter
authors.forename: Audrey
authors.initials: A
authors.lastname: Cordi
authors.forename: Simonetta
authors.initials: S
authors.lastname: Gribaldo
authors.forename: Sébastien
authors.initials: S
authors.lastname: Gallien
authors.forename: Florence
authors.initials: F
authors.lastname: Goulhen-Chollet
authors.forename: Audrey
authors.initials: A
authors.lastname: Heinrich-Salmeron
authors.forename: Christine
authors.initials: C
authors.lastname: Carapito
authors.forename: Christophe
authors.initials: C
authors.lastname: Pagnout
authors.forename: Didier
authors.initials: D
authors.lastname: Montaut
authors.forename: Fabienne
authors.initials: F
authors.lastname: Seby
authors.forename: Alain
authors.initials: A
authors.lastname: Van Dorsselaer
authors.forename: Christine
authors.initials: C
authors.lastname: Schaeffer
authors.forename: Philippe N
authors.initials: PN
authors.lastname: Bertin
authors.forename: Pascale
authors.initials: P
authors.lastname: Bauda
authors.forename: Florence
authors.initials: F
authors.lastname: Arsène-Ploetze
mesh_terms: D000222:Adaptation, Physiological; D001105:Archaea / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D001151:Arsenic / Q000378:metabolism*; D001419:Bacteria / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D044822:Biodiversity; D019641:DNA, Archaeal / Q000032:analysis / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D017753:Ecosystem; D004785:Environmental Pollutants / Q000378:metabolism; D005602:France; D022761:Gene Transfer, Horizontal; D019015:Geologic Sediments / Q000382:microbiology*; D059013:Microbial Consortia / Q000502:physiology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D040901:Proteomics; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000235:genetics; D045483:Rivers / Q000382:microbiology*
chemical_list: D019641:DNA, Archaeal; D004269:DNA, Bacterial; D004785:Environmental Pollutants; D012336:RNA, Ribosomal, 16S; D001151:Arsenic