title: Gold nanoparticle-mediated gene delivery induces widespread changes in the expression of innate immunity genes.
abstract: The unique properties of oligonucleotide (and small interfering RNA)-modified gold nanoparticle conjugates make them promising intracellular gene regulation agents. We found that gold nanoparticles stably functionalized with covalently attached oligonucleotides activate immune-related genes and pathways in human peripheral blood mononuclear cells, but not an immortalized, lineage-restricted cell line. These findings have strong implications for the application of oligonucleotide-modified gold nanoparticle conjugates in translational research and in the development of therapeutics and gene delivery systems.
authors.affiliation: Division of Infectious Diseases, The Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA.
authors.forename: E-Y
authors.initials: EY
authors.lastname: Kim
authors.forename: R
authors.initials: R
authors.lastname: Schulz
authors.forename: P
authors.initials: P
authors.lastname: Swantek
authors.forename: K
authors.initials: K
authors.lastname: Kunstman
authors.forename: M H
authors.initials: MH
authors.lastname: Malim
authors.forename: S M
authors.initials: SM
authors.lastname: Wolinsky
mesh_terms: D001692:Biological Transport; D002460:Cell Line; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D018014:Gene Transfer Techniques; D006046:Gold; D006801:Humans; D007113:Immunity, Innate / Q000235:genetics*; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D053768:Metal Nanoparticles; D009841:Oligonucleotides / Q000737:chemistry / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D009841:Oligonucleotides; D006046:Gold