title: APOA4 polymorphism as a risk factor for unfavorable lipid serum profile and depression: a cross-sectional study.
abstract: BACKGROUND
  APOA1/C3/A4/A5 gene cluster is closely involved in lipid metabolism, and its polymorphisms have been associated with coronary heart disease and lipid plasma levels. Here, we aimed to investigate associations of APOC3 (3238C>G, -482C>T, 1100C>T) and APOA4 (Gln360His, Thr347Ser) polymorphisms in 382 individuals from a cohort of a Longitudinal Brazilian Elderly Study with major age-related morbidities and with lipid and protein serum levels.
  
  
  METHODS
  The whole sample was genotyped by polymerase chain reaction-restriction fragment length polymorphism. Descriptive statistics, logistic regression analysis, Student t test, deviation from Hardy-Weinberg, Bonferroni correction for multiple testing, and haplotype analyses were performed.
  
  
  RESULTS
  Although APOC3 1100T allele carriers presented lower triglyceride and very low density lipoprotein levels than non-T carriers, these associations disappeared after Bonferroni correction (P > 0.05). Moreover, APOA4 360His allele was associated with depression (P = 0.03), increased triglyceride (P = 0.035) and very low density lipoprotein (P = 0.035) levels, and reduced HDL levels (P = 0.0005). Haplotype analyses found an association between His/C/C haplotype (Gln360His/-482C>T/1100C>T) with depression, but this result was due to Gln360His polymorphism.
  
  
  CONCLUSIONS
  Our data suggest that 360His allele might be a risk factor for depression and unfavorable lipid profile and depression for elderly people in the Brazilian population.
authors.affiliation: Disciplina de Genética, Departamento de Morfologia e Genética, São Paulo, Brazil. anessakaota@gmail.com
authors.forename: Vanessa Kiyomi
authors.initials: VK
authors.lastname: Ota
authors.forename: Elizabeth Suchi
authors.initials: ES
authors.lastname: Chen
authors.forename: Tatiana Flank
authors.initials: TF
authors.lastname: Ejchel
authors.forename: Tatiane Katsue
authors.initials: TK
authors.lastname: Furuya
authors.forename: Diego Robles
authors.initials: DR
authors.lastname: Mazzotti
authors.forename: Maysa Seabra
authors.initials: MS
authors.lastname: Cendoroglo
authors.forename: Luiz Roberto
authors.initials: LR
authors.lastname: Ramos
authors.forename: Lara Quirino
authors.initials: LQ
authors.lastname: Araujo
authors.forename: Rommel Rodriguez
authors.initials: RR
authors.lastname: Burbano
authors.forename: Marília de Arruda Cardoso
authors.initials: Mde A
authors.lastname: Smith
mesh_terms: D000368:Aged; D000483:Alleles; D001054:Apolipoproteins A / Q000097:blood / Q000235:genetics*; D001938:Brazil; D003430:Cross-Sectional Studies; D003863:Depression / Q000097:blood / Q000235:genetics; D005260:Female; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D008055:Lipids / Q000097:blood*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012307:Risk Factors; D014280:Triglycerides / Q000235:genetics / Q000378:metabolism
chemical_list: D001054:Apolipoproteins A; D008055:Lipids; D014280:Triglycerides; C022205:apolipoprotein A-IV