title: Interphase chromosome structures of human cells.
abstract: Major intermediates of chromosome condensation in erythroleukemia K562 cells are presented. Interphase chromatin structures became visible after reversal of permeabilization. Large-scale chromatin structures and the development of individual interphase chromosomes were observed by fluorescence microscopy. In the linear arrangement the following major intermediates of K562 chromatin condensation could be distinguished: (1) the most decondensed chromatin veil, (2) chromatin ribbon, (3) chromatin funnel, a new intermediate regarded as the earliest visible form of interphase chromosomes, (4) chromatin body, (5) 300 nm chromatin fiber, (6) u, v, or s forms of chromosomes, and (7) linear chromosomes. The observations made in nuclei of K562 cells conform to the model of helical coil chromosome condensation.
authors.affiliation: Department of Microbial Biotechnology and Cell Biology, University of Debrecen, Debrecen, Hungary. bgaspar@delfin.klte.hu
authors.forename: Gaspar
authors.initials: G
authors.lastname: Banfalvi
authors.forename: Gabor
authors.initials: G
authors.lastname: Nagy
mesh_terms: D002843:Chromatin / Q000648:ultrastructure; D002877:Chromosomes, Human / Q000648:ultrastructure*; D006801:Humans; D007399:Interphase; D020014:K562 Cells; D008856:Microscopy, Fluorescence; D008957:Models, Genetic
chemical_list: D002843:Chromatin