title: Tissue engineering scaffolds containing embedded fluorinated-zeolite oxygen vectors.
abstract: Efficient oxygen supply is a continuing challenge for the fabrication of successful tissue engineered constructs with clinical relevance. In an effort to enhance oxygen delivery we report the feasibility of using fluorinated zeolite particles embedded in three-dimensional (3-D) polyurethane scaffolds as novel oxygen vectors. First, 1H,1H,2H,2H-perfluorodecyltriethoxysilane was successfully coupled to zeolite framework particles to examine the dose-dependent dissolved oxygen concentration. Following this, the fluorinated-zeolite (FZ) particles were embedded in 3-D tissue engineering polyurethane scaffolds. Our data demonstrates an even distribution of FZ particles in the 3-D scaffolds without affecting the scaffold porosity or pore size. Human coronary artery smooth muscle cell (HCASMC) proliferation on FZ-containing polyurethane (PCU-FZ) scaffolds was significantly greater than on control scaffolds (P=0.05). Remarkably, cell infiltration depths on the PCU-FZ scaffolds was double that on PCU control scaffolds. Taken together, our data suggest the potential of PCU-FZ scaffolds for tissue engineering with enhanced oxygen delivery to cells.
authors.affiliation: Department of Chemical and Biochemical Engineering, University of Western Ontario, London, Ontario, Canada N5A 5B9.
authors.forename: Dawit G
authors.initials: DG
authors.lastname: Seifu
authors.forename: Tayirjan T
authors.initials: TT
authors.lastname: Isimjan
authors.forename: Kibret
authors.initials: K
authors.lastname: Mequanint
mesh_terms: D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003331:Coronary Vessels / Q000166:cytology; D054879:Halogenation / Q000187:drug effects; D006801:Humans; D008628:Mercury / Q000737:chemistry; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000187:drug effects; D010100:Oxygen / Q000737:chemistry*; D056951:Photoelectron Spectroscopy; D011140:Polyurethanes / Q000494:pharmacology; D016062:Porosity / Q000187:drug effects; D013052:Spectrometry, X-Ray Emission; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D017641:Zeolites / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D011140:Polyurethanes; D017641:Zeolites; D008628:Mercury; D010100:Oxygen