title: Modulation of chromatin modifying factors' gene expression in embryonic and induced pluripotent stem cells.
abstract: Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) are a promising source of cells for regenerative medicine because of their potential of self renew and differentiation. Multiple evidences highlight the relationship of chromatin remodeling with stem cell properties, differentiation programs and reprogramming for iPSC obtention. With the purpose of finding chromatin modifying factors relevant to these processes, and based on ChIP on chip studies, we selected several genes that could be modulated by Oct4, Sox2 and Nanog, critical transcription factors in stem cells, and studied their expression profile along the differentiation in mouse and human ESCs, and in mouse iPSCs. In this work, we analyzed the expression of Gcn5l2, GTF3C3, TAF15, ATF7IP, Myst2, HDAC2, HDAC3, HDAC5, HDAC10, SUV39H2, Jarid2, and Bmi-1. We found some genes from different functional groups that were highly modulated, suggesting that they could be relevant both in the undifferentiated state and during differentiation. These findings could contribute to the comprehension of molecular mechanisms involved in pluripotency, early differentiation and reprogramming. We believe that a deeper knowledge of the epigenetic regulation of ESC will allow improving somatic cell reprogramming for iPSC obtention and differentiation protocols optimization.
authors.affiliation: Laboratorio de Regulación de Expresión Génica en el Crecimiento, Supervivencia y Diferenciación Celular, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina. carlosluzzani@qb.fcen.uba.ar
authors.forename: Carlos
authors.initials: C
authors.lastname: Luzzani
authors.forename: Claudia
authors.initials: C
authors.lastname: Solari
authors.forename: Noelia
authors.initials: N
authors.lastname: Losino
authors.forename: Waisman
authors.initials: W
authors.lastname: Ariel
authors.forename: Leonardo
authors.initials: L
authors.lastname: Romorini
authors.forename: Carolina
authors.initials: C
authors.lastname: Bluguermann
authors.forename: Gustavo
authors.initials: G
authors.lastname: Sevlever
authors.forename: Lino
authors.initials: L
authors.lastname: Barañao
authors.forename: Santiago
authors.initials: S
authors.lastname: Miriuka
authors.forename: Alejandra
authors.initials: A
authors.lastname: Guberman
mesh_terms: D000818:Animals; D002460:Cell Line; D002843:Chromatin / Q000235:genetics*; D047369:Chromatin Immunoprecipitation; D053595:Embryonic Stem Cells / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000378:metabolism*; D051379:Mice; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D002843:Chromatin; D014157:Transcription Factors