title: Magnetoliposomes with high USPIO entrapping efficiency, stability and magnetic properties.
abstract: The DRV technique (followed by extrusion) was used for construction of hydrophilic-USPIO encapsulating liposomes. Magnetoliposomes (ML) were characterized for size, surface charge, entrapment, physical stability and magnetic properties (relaxivity). Results show that nanosized extruded-DRV MLs encapsulate higher amounts of USPIOs in comparison with sonicated vesicles. Fe (III) encapsulation efficiency (EE) is 12%, the highest reported to date for nanosized MLs. EE of MLs is influenced by ML membrane composition and polyethyleneglycol (PEG) coating. PEG-coating increases ML EE and stability; however, r(2)-to-r(1) ratios decrease (in comparison with non-PEGylated MLs). Most ML-types are efficient T2 contrast agents (because r(2)-to-r(1) ratios are higher than that of free USPIOs). Targeted MLs were formed by successfully immobilizing OX-26 monoclonal antibody on ML surface (biotin-streptavidin ligation), without significant loss of USPIOs. Targeted MLs retained their nanosize and integrity during storage for 1 month at 4 °C and up to 2 weeks at 37 °C.
authors.affiliation: Laboratory of Pharmaceutical Technology, Dept. of Pharmacy, School of Health Sciences, University of Patras, Patras, Greece.
authors.forename: Athanasios
authors.initials: A
authors.lastname: Skouras
authors.forename: Spyridon
authors.initials: S
authors.lastname: Mourtas
authors.forename: Eleni
authors.initials: E
authors.lastname: Markoutsa
authors.forename: Marie-Christine
authors.initials: MC
authors.lastname: De Goltstein
authors.forename: Claire
authors.initials: C
authors.lastname: Wallon
authors.forename: Sarah
authors.initials: S
authors.lastname: Catoen
authors.forename: Sophia G
authors.initials: SG
authors.lastname: Antimisiaris
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry; D000911:Antibodies, Monoclonal / Q000737:chemistry; D003287:Contrast Media / Q000737:chemistry*; D003911:Dextrans / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D008055:Lipids / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry*; D058185:Magnetite Nanoparticles / Q000737:chemistry*
chemical_list: D055910:Antibodies, Immobilized; D000911:Antibodies, Monoclonal; D003287:Contrast Media; D003911:Dextrans; D008055:Lipids; D008081:Liposomes; D058185:Magnetite Nanoparticles; C097921:ferumoxtran-10; D006046:Gold