title: An advance in proline ligation.
abstract: Native chemical ligation (NCL) is widely applicable for building proteins in the laboratory. Since the discovery of this method, many strategies have been developed to enhance its capability and efficiency. Because of the poor reactivity of proline thioesters, ligation at a C-terminal proline site is not readily accomplished. Here, we demonstrate that ligation at an N-terminal protein is feasible using the combined logic of NCL and metal-free dethiylation (MFD).
authors.affiliation: Laboratory for Bioorganic Chemistry, Sloan-Kettering Institute for Cancer Research, 1275 York Avenue, New York, New York 10065, USA.
authors.forename: Shiying
authors.initials: S
authors.lastname: Shang
authors.forename: Zhongping
authors.initials: Z
authors.lastname: Tan
authors.forename: Suwei
authors.initials: S
authors.lastname: Dong
authors.forename: Samuel J
authors.initials: SJ
authors.lastname: Danishefsky
mesh_terms: D011392:Proline / Q000737:chemistry*; D011506:Proteins / Q000138:chemical synthesis / Q000737:chemistry; D013237:Stereoisomerism; D013455:Sulfur / Q000737:chemistry
chemical_list: D011506:Proteins; D013455:Sulfur; D011392:Proline