title: Variations in BARE-1 insertion patterns in barley callus cultures.
abstract: The stability of aging barley calli was investigated with the barley retroelement 1 (BARE-1) retrotransposon specific inter-retrotransposon amplified polymorphism (IRAP) technique. Mature embryos of barley (Hordeum vulgare cv. Zafer-160) were cultured on callus induction MS medium supplemented with 3 mg/L 2,4-D and maintained on the same medium for 60 days. Ten IRAP primers were used in 25 different combinations. The similarity index between 30-day-old and 45-day-old calli was 84%; however, the similarity index between mature embryos and 45-day-old calli was 75%. These culture conditions caused BARE-1 retrotransposon alterations to appear as different band profiles. This is the first report of the use of the IRAP technique in barley in an investigation of callus development.
authors.affiliation: Department of Molecular Biology and Genetics, Faculty of Science, Istanbul University, Vezneciler, Istanbul, Turkey.
authors.forename: C
authors.initials: C
authors.lastname: Evrensel
authors.forename: S
authors.initials: S
authors.lastname: Yilmaz
authors.forename: A
authors.initials: A
authors.lastname: Temel
authors.forename: N
authors.initials: N
authors.lastname: Gozukirmizi
mesh_terms: D001483:Base Sequence; D003470:Culture Media; D017931:DNA Primers; D014644:Genetic Variation; D001467:Hordeum / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D018626:Retroelements
chemical_list: C082800:BARE-1 protein, Hordeum vulgare; D003470:Culture Media; D017931:DNA Primers; D010940:Plant Proteins; D018626:Retroelements