title: The effects of oral Ginkgo biloba supplementation on radiation-induced oxidative injury in the lens of rat.
abstract: BACKGROUND
  The aim of this study was to evaluate the antioxidant role of Ginkgo biloba (GB) against radiation-induced cataract in the rat lens after total cranial irradiation with a single 5 Gray (Gy) dose of gamma irradiation.
  
  
  METHODS
  Twenty-four Sprague-Dawley rats were used for the experiment. The rats were randomly divided into three equal groups. Group 1 did not receive GB or irradiation (control group) but received 1-ml saline orally plus sham-irradiation. Group 2 received total cranium 5 Gy of gamma irradiation as a single dose (IR group) plus 1-ml saline orally. Group 3 received total cranium irradiation plus 40 mg/kg/day GBE (IR plus GBE group). Biochemical parameters measured in murine lenses were carried out using spectrophotometric techniques.
  
  
  RESULTS
  Lens total (enzymatic plus non-enzymatic) superoxide scavenger activity (TSSA), non-enzymatic superoxide scavenger activity (NSSA), glutathione reductase (GRD), and glutathione-S- transferase (GST) activities significantly increased in the IR plus GBE groups when compared with the IR group. However, TSSA, GRD and GST activities were significantly lower in the IR group when compared with the control group. Lens xanthine oxidase (XO) activity in the IR group significantly increased compared to that of both the control and IR plus GBE groups.
  
  
  CONCLUSIONS
  GBE has clear antioxidant properties and is likely to be a valuable drug for protection against gamma-irradiation and/or be used as an antioxidant against oxidative stress.
authors.affiliation: Department of Ophthalmology, Gaziantep University, Medical School, Gaziantep, Turkey.
authors.forename: Seydi
authors.initials: S
authors.lastname: Okumus
authors.forename: Seyithan
authors.initials: S
authors.lastname: Taysi
authors.forename: Mustafa
authors.initials: M
authors.lastname: Orkmez
authors.forename: Edibe
authors.initials: E
authors.lastname: Saricicek
authors.forename: Elif
authors.initials: E
authors.lastname: Demir
authors.forename: Mustafa
authors.initials: M
authors.lastname: Adli
authors.forename: Behcet
authors.initials: B
authors.lastname: Al