title: Cloning, structural characterization and expression analysis of a novel lipid storage droplet protein-1 (LSD-1) gene in Chinese honeybee (Apis cerana cerana).
abstract: Lipid storage droplet 1 (LSD-1), a PAT family protein located around lipid droplets in insects, is intimately linked to lipid droplets formation and lipid metabolism. Conjugated linoleic acid (CLA) and rosiglitazone (Rosi) have previously been shown to modulate the expression of several PAT family proteins through peroxisome proliferator-activated receptor-γ (PPARγ). In the present study, we isolated and characterized a novel LSD-1 gene, referred to AccLSD-1, from Chinese honeybee (Apis cerana cerana). Sequence analysis indicated that the central region of LSD-1 protein had significant sequence similarity and a typical LSD-1 gene was composed of 8 exons and 7 introns. Interestingly, the first intron of AccLSD-1 including several PPARγ-response elements (PPREs) was located in 5' UTR. Analysis of 5'-flanking region of AccLSD-1 revealed a number of putative cis-acting elements, including three PPREs. Quantitative real-time PCR showed that AccLSD-1 expressed ubiquitously from feeding larva to adult, and its expression level was highest at brown-eyed pupae (Pb) stage. The effect of CLA, Rosi and combination on AccLSD-1 expressions indicated 1% CLA and 0.5 mg/ml Rosi were considered as the suitable diets for rearing adult workers in laboratory, and AccLSD-1 was down-regulated by CLA whereas up-regulated by Rosi. Furthermore, the combination of CLA and Rosi remarkly rescued the suppression of AccLSD-1 expression by CLA alone. These results suggest that AccLSD-1 is associated with A. cerana cerana development, especially during pupal metamorphosis, and can be regulated by CLA or Rosi possibly via activating PPARγ.
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Taian, 271018 Shandong, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Gong
authors.forename: Xingqi
authors.initials: X
authors.lastname: Guo
authors.forename: Baohua
authors.initials: B
authors.lastname: Xu
mesh_terms: D000818:Animals; D001483:Base Sequence; D001516:Bees / Q000235:genetics* / Q000254:growth & development; D003001:Cloning, Molecular; D016000:Cluster Analysis; D017931:DNA Primers / Q000235:genetics; D040461:Gene Components; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D044243:Linoleic Acids, Conjugated / Q000494:pharmacology; D050356:Lipid Metabolism / Q000235:genetics*; D008969:Molecular Sequence Data; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D010802:Phylogeny; D011679:Pupa / Q000254:growth & development; D060888:Real-Time Polymerase Chain Reaction; D020218:Response Elements / Q000235:genetics; D000077154:Rosiglitazone; D017422:Sequence Analysis, DNA; D045162:Thiazolidinediones / Q000494:pharmacology
chemical_list: D017931:DNA Primers; D019476:Insect Proteins; D044243:Linoleic Acids, Conjugated; D047495:PPAR gamma; D045162:Thiazolidinediones; D000077154:Rosiglitazone