title: High-resolution genotyping of Chlamydia trachomatis by use of a novel multilocus typing DNA microarray.
abstract: Typing of Chlamydia trachomatis is important to understanding its epidemiology. Currently used methods such as DNA sequencing of the ompA gene and multilocus sequence typing (MLST) either offer limited epidemiological resolution or are laborious and expensive, or both. DNA microarray technology using the ArrayStrip format is an affordable alternative for genotyping. In this study, we developed a new multilocus typing (MLT) DNA microarray, based on the target regions of a high-resolution MLST system as well as software for easy analysis. Validation of the array was done by typing 80 previously MLST-typed clinical specimens from unselected adolescents in school. The MLT array showed 100% specificity and provided 2.4-times-higher resolution than ompA sequencing, separating the commonly predominating ompA E/Bour genotype into 7 MLT array genotypes. The MLT array reproduced epidemiological findings revealed by the MLST system and showed sufficient sensitivity to work with clinical specimens. Compared to MLST analysis, the expenses needed for testing a sample with the MLT array are considerably lower. Moreover, testing can be completed within 1 working day rather than 3 or 4 days, with data analysis not requiring highly specialized personnel. The present MLT array represents a powerful alternative in C. trachomatis genotyping.
authors.affiliation: Section of Clinical Bacteriology, Department of Medical Sciences, Uppsala University, S-751 85 Uppsala, Sweden.
authors.forename: Linus
authors.initials: L
authors.lastname: Christerson
authors.forename: Anke
authors.initials: A
authors.lastname: Ruettger
authors.forename: Kirsten
authors.initials: K
authors.lastname: Gravningen
authors.forename: Ralf
authors.initials: R
authors.lastname: Ehricht
authors.forename: Konrad
authors.initials: K
authors.lastname: Sachse
authors.forename: Björn
authors.initials: B
authors.lastname: Herrmann
mesh_terms: D000293:Adolescent; D002690:Chlamydia Infections / Q000382:microbiology*; D002692:Chlamydia trachomatis / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D005838:Genotype; D006801:Humans; D008297:Male; D017720:Molecular Epidemiology / Q000379:methods; D058889:Molecular Typing / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012680:Sensitivity and Specificity