title: Distinct microRNA signatures in human lymphocyte subsets and enforcement of the naive state in CD4+ T cells by the microRNA miR-125b.
abstract: MicroRNAs are small noncoding RNAs that regulate gene expression post-transcriptionally. Here we applied microRNA profiling to 17 human lymphocyte subsets to identify microRNA signatures that were distinct among various subsets and different from those of mouse lymphocytes. One of the signature microRNAs of naive CD4+ T cells, miR-125b, regulated the expression of genes encoding molecules involved in T cell differentiation, including IFNG, IL2RB, IL10RA and PRDM1. The expression of synthetic miR-125b and lentiviral vectors encoding the precursor to miR-125b in naive lymphocytes inhibited differentiation to effector cells. Our data provide an 'atlas' of microRNA expression in human lymphocytes, define subset-specific signatures and their target genes and indicate that the naive state of T cells is enforced by microRNA.
authors.affiliation: Istituto Nazionale Genetica Molecolare, Milano, Italy.
authors.forename: Riccardo L
authors.initials: RL
authors.lastname: Rossi
authors.forename: Grazisa
authors.initials: G
authors.lastname: Rossetti
authors.forename: Lynn
authors.initials: L
authors.lastname: Wenandy
authors.forename: Serena
authors.initials: S
authors.lastname: Curti
authors.forename: Anna
authors.initials: A
authors.lastname: Ripamonti
authors.forename: Raoul J P
authors.initials: RJ
authors.lastname: Bonnal
authors.forename: Roberto Sciarretta
authors.initials: RS
authors.lastname: Birolo
authors.forename: Monica
authors.initials: M
authors.lastname: Moro
authors.forename: Maria C
authors.initials: MC
authors.lastname: Crosti
authors.forename: Paola
authors.initials: P
authors.lastname: Gruarin
authors.forename: Stefano
authors.initials: S
authors.lastname: Maglie
authors.forename: Francesco
authors.initials: F
authors.lastname: Marabita
authors.forename: Debora
authors.initials: D
authors.lastname: Mascheroni
authors.forename: Valeria
authors.initials: V
authors.lastname: Parente
authors.forename: Mario
authors.initials: M
authors.lastname: Comelli
authors.forename: Emilio
authors.initials: E
authors.lastname: Trabucchi
authors.forename: Raffaele
authors.initials: R
authors.lastname: De Francesco
authors.forename: Jens
authors.initials: J
authors.lastname: Geginat
authors.forename: Sergio
authors.initials: S
authors.lastname: Abrignani
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Pagani
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology; D019295:Computational Biology / Q000379:methods; D005434:Flow Cytometry; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000276:immunology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016176:T-Lymphocyte Subsets / Q000276:immunology*
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger