title: Proteomic analysis of global changes in protein expression during exposure of gamma radiation in Bacillus sp. HKG 112 isolated from saline soil.
abstract: A Gram-positive bacterium was isolated from the saline soils of Jangpura (U.P.), India, and showed high-level of radiation-resistant property and survived upto 12.5 kGy dose of gamma radiation. The 16S rDNA sequence of this strain was examined, identified as Bacillus sp. strain HKG 112, and was submitted to the NCBI GenBank (Accession No. GQ925432). The mechanism of radiation resistance and gene level expression were examined by proteomic analysis of whole-cell extract. Two proteins, 38 kDa and 86.5 kDa excised from SDS-PAGE, which showed more significant changes after radiation exposure, were identified by MALDI-TOF as being flagellin and S-layer protein, respectively. Twenty selected 2-DE protein spots from the crude extracts of Bacillus sp. HKG 112, excised from 2- DE, were identified by liquid chromatography mass spectrometry (LC-MS) out of which 16 spots showed significant changes after radiation exposure and might be responsible for the radiation resistance property. Our results suggest that the different responses of some genes under radiation for the expression of radiation-dependent proteins could contribute to a physiological advantage and would be a significant initial step towards a full-system understanding of the radiation stress protection mechanisms of bacteria in different environments.
authors.affiliation: Division of Comparative Genomics, Institute of Genomics and Integrative Biology, Mall Road, New Delhi, India.
authors.forename: Anil Kumar
authors.initials: AK
authors.lastname: Gupta
authors.forename: Rajiv
authors.initials: R
authors.lastname: Pathak
authors.forename: Bharat
authors.initials: B
authors.lastname: Singh
authors.forename: Hemlata
authors.initials: H
authors.lastname: Gautam
authors.forename: Ram
authors.initials: R
authors.lastname: Kumar
authors.forename: Raj
authors.initials: R
authors.lastname: Kumar
authors.forename: Rajesh
authors.initials: R
authors.lastname: Arora
authors.forename: Hemant
authors.initials: H
authors.lastname: Gautam
mesh_terms: D001407:Bacillus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000528:radiation effects*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D005408:Flagellin / Q000235:genetics / Q000378:metabolism; D005720:Gamma Rays; D007194:India; D013058:Mass Spectrometry; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D040901:Proteomics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000737:chemistry*; D012987:Soil / Q000032:analysis; D012988:Soil Microbiology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D001426:Bacterial Proteins; D008562:Membrane Glycoproteins; D012336:RNA, Ribosomal, 16S; C055764:S-layer proteins; D012987:Soil; D005408:Flagellin; D012965:Sodium Chloride