title: E4F1 deficiency results in oxidative stress-mediated cell death of leukemic cells.
abstract: The multifunctional E4F1 protein was originally discovered as a target of the E1A viral oncoprotein. Growing evidence indicates that E4F1 is involved in key signaling pathways commonly deregulated during cell transformation. In this study, we investigate the influence of E4F1 on tumorigenesis. Wild-type mice injected with fetal liver cells from mice lacking CDKN2A, the gene encoding Ink4a/Arf, developed histiocytic sarcomas (HSs), a tumor originating from the monocytic/macrophagic lineage. Cre-mediated deletion of E4F1 resulted in the death of HS cells and tumor regression in vivo and extended the lifespan of recipient animals. In murine and human HS cell lines, E4F1 inactivation resulted in mitochondrial defects and increased production of reactive oxygen species (ROS) that triggered massive cell death. Notably, these defects of E4F1 depletion were observed in HS cells but not healthy primary macrophages. Short hairpin RNA-mediated depletion of E4F1 induced mitochondrial defects and ROS-mediated death in several human myeloid leukemia cell lines. E4F1 protein is overexpressed in a large subset of human acute myeloid leukemia samples. Together, these data reveal a role for E4F1 in the survival of myeloid leukemic cells and support the notion that targeting E4F1 activities might have therapeutic interest.
authors.affiliation: Institut de Génétique Moléculaire de Montpellier, Centre National de la Recherche Scientifique Unité Mixte de Recherche 5535, Institut Fédératif de Recherche 122, Université de Montpellier, Montpellier 34293, France.
authors.forename: Elodie
authors.initials: E
authors.lastname: Hatchi
authors.forename: Genevieve
authors.initials: G
authors.lastname: Rodier
authors.forename: Matthieu
authors.initials: M
authors.lastname: Lacroix
authors.forename: Julie
authors.initials: J
authors.lastname: Caramel
authors.forename: Olivier
authors.initials: O
authors.lastname: Kirsh
authors.forename: Chantal
authors.initials: C
authors.lastname: Jacquet
authors.forename: Emilie
authors.initials: E
authors.lastname: Schrepfer
authors.forename: Sylviane
authors.initials: S
authors.lastname: Lagarrigue
authors.forename: Laetitia Karine
authors.initials: LK
authors.lastname: Linares
authors.forename: Gwendaline
authors.initials: G
authors.lastname: Lledo
authors.forename: Sylvie
authors.initials: S
authors.lastname: Tondeur
authors.forename: Pierre
authors.initials: P
authors.lastname: Dubus
authors.forename: Claude
authors.initials: C
authors.lastname: Sardet
authors.forename: Laurent
authors.initials: L
authors.lastname: Le Cam
mesh_terms: D000818:Animals; D001343:Autophagy / Q000502:physiology; D001483:Base Sequence; D016923:Cell Death / Q000502:physiology; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic; D004268:DNA-Binding Proteins / Q000037:antagonists & inhibitors / Q000172:deficiency* / Q000235:genetics; D054747:Histiocytic Sarcoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000378:metabolism* / Q000473:pathology*; D008264:Macrophages / Q000378:metabolism / Q000473:pathology; D051379:Mice; D057507:Mice, 129 Strain; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D018345:Mice, Knockout; D008822:Mice, Transgenic; D018384:Oxidative Stress; D034741:RNA, Small Interfering / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism; D012097:Repressor Proteins / Q000037:antagonists & inhibitors / Q000172:deficiency* / Q000235:genetics; D015398:Signal Transduction; D014157:Transcription Factors / Q000037:antagonists & inhibitors / Q000172:deficiency* / Q000235:genetics; D044767:Ubiquitin-Protein Ligases
chemical_list: D004268:DNA-Binding Proteins; D034741:RNA, Small Interfering; D017382:Reactive Oxygen Species; D012097:Repressor Proteins; D014157:Transcription Factors; C513814:E4F1 protein, human; C084953:E4f1 protein, mouse; D044767:Ubiquitin-Protein Ligases