title: Protein expression of mesenchymal stem cells after transfection of pcDNA3.1⁻-hVEGF₁₆₅ by ultrasound-targeted microbubble destruction.
abstract: Ultrasound-targeted microbubble destruction (UTMD) has been proposed as a new technique for organ-specific gene transfer and drug delivery. This study was performed to investigate the effect of UTMD on marrow mesenchymal stem cells (MSCs) transfected with pcDNA3.1⁻-hVEGF₁₆₅.pcDNA3.1⁻-hVEGF₁₆₅ were transfected into the third passage of MSCs, with or without UTMD under different ultrasound conditions. Protein expression was quantified by hVEGF₁₆₅-ELISA kit after transfection for 24, 48, and 72 hours. UTMD-mediated transfection of MSCs yielded a significant protein expression. UTMD of mechanic index (MI) 0.6 for 90 seconds led to the highest level of protein expression.
authors.affiliation: Department of Ultrasound, Second Affiliated Hospital, Zhejiang University College of Medicine, 88 Jiefang Road, Hangzhou 310009, China.
authors.forename: Zhaoxia
authors.initials: Z
authors.lastname: Pu
authors.forename: Xiangdong
authors.initials: X
authors.lastname: You
authors.forename: Qiyuan
authors.initials: Q
authors.lastname: Xu
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Xie
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.forename: Wang
authors.initials: W
authors.lastname: Jian'an
mesh_terms: D000818:Animals; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D009928:Organ Specificity; D051381:Rats; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering; D014162:Transfection / Q000379:methods*; D014465:Ultrasonics; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D018076:DNA, Complementary; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A