title: Membrane-based inverse transition cycling: an improved means for purifying plant-derived recombinant protein-elastin-like polypeptide fusions.
abstract: Elastin-like peptide (ELP) was fused to two different avian flu H5N1 antigens and expressed in transgenic tobacco plants. The presence of the ELP tag enhanced the accumulation of the heterologous proteins in the tobacco leaves. An effective membrane-based Inverse Transition Cycling was developed to recover the ELPylated antigens and antibodies from plant material. The functionality of both the ELPylated neuraminidase and an ELPylated nanobody was demonstrated.
authors.affiliation: Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstrasse 3, Gatersleben 06466, Germany; E-Mail: hoang@ipk-gatersleben.de.
authors.forename: Hoang Trong
authors.initials: HT
authors.lastname: Phan
authors.forename: Udo
authors.initials: U
authors.lastname: Conrad
mesh_terms: D000956:Antigens, Viral / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D004549:Elastin / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics*; D009439:Neuraminidase / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000378:metabolism*; D011993:Recombinant Fusion Proteins; D014026:Nicotiana / Q000235:genetics; D014170:Transformation, Genetic
chemical_list: D000956:Antigens, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D011993:Recombinant Fusion Proteins; C109963:hemagglutinin, avian influenza A virus; D004549:Elastin; D009439:Neuraminidase