title: The radioprotective effect of Zataria multiflora against genotoxicity induced by γ irradiation in human blood lymphocytes.
abstract: The radioprotective effect of hydroalcholic Zataria multiflora (Avishan-e shirazi) extract was investigated against genotoxicity induced by γ irradiation in human lymphocytes. Peripheral blood samples were collected from human volunteers and incubated with Z. multiflora extract at different concentrations (5, 10, and 50 μg/mL) for 1 hour. At each dose point, the whole blood was exposed in vitro to 150 cGy of cobalt-60 γ irradiation, and then the lymphocytes were cultured with mitogenic stimulation to determine number of the micronuclei in cytokinesis-blocked binucleated cells. The treatment of lymphocytes with extract showed a significant decrease in the incidence of micronuclei binucleated cells, compared with similarly irradiated lymphocytes without extract against γ irradiation. The maximum protection and decrease in frequency of micronuclei was observed at 50 μg/mL of Zataria extract by 32% reduction. High-performance liquid chromatography analysis of extract showed that it contains high amounts of thymol. Zataria extract exhibited concentration-dependent radical-scavenging activity on 1,1-diphenyl-2-picryl hydrazyl free radicals. These data have an important application for the protection of human lymphocyte from the genetic damage and side-effects induced by γ irradiation in personnel exposed to radiation.
authors.affiliation: Department of Radiopharmacy, Traditional and Complementary Medicine Research Center, Mazandaran University of Medical Sciences, Sari, Iran. jhosseinim@yahoo.com
authors.forename: Seyed Jalal
authors.initials: SJ
authors.lastname: Hosseinimehr
authors.forename: Aziz
authors.initials: A
authors.lastname: Mahmoudzadeh
authors.forename: Amirhossein
authors.initials: A
authors.lastname: Ahmadi
authors.forename: Saeb Ahmad
authors.initials: SA
authors.lastname: Ashrafi
authors.forename: Nayereh
authors.initials: N
authors.lastname: Shafaghati
authors.forename: Narges
authors.initials: N
authors.lastname: Hedayati
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003037:Cobalt Radioisotopes / Q000494:pharmacology; D004249:DNA Damage / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D029624:Ferns / Q000378:metabolism*; D005609:Free Radicals; D005720:Gamma Rays; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects* / Q000528:radiation effects*; D015162:Micronucleus Tests / Q000379:methods; D010936:Plant Extracts / Q000494:pharmacology*; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D000975:Antioxidants; D003037:Cobalt Radioisotopes; D005609:Free Radicals; D010936:Plant Extracts; D011837:Radiation-Protective Agents