title: Nestin expression in Bowen's disease and Bowen's carcinoma associated with human papillomavirus.
abstract: Human papillomaviruses (HPVs) have been detected in lesions of Bowen's disease (BD) and Bowen's carcinoma (BC); the invasive tumor retains the cytological characteristics of BD. Previous reports suggest that nestin-expressing hair follicle stem cells are undifferentiated and pluripotent, and nestin expression in some tumors indicates poor differentiation and high grade of malignancy. We identified HPV-DNA in BD (n=25) and BC (n=23) by in situ hybridization (ISH) analysis with INFORM(®) HPV III (Ventana Medical Systems. AZ, USA) and determined nestin expression by indirect immunohistochemical staining with anti-nestin polyclonal antibody (IBL, Gunma, Japan). We detected HPV-DNA in 68% of BD and in 87% of BC. In BD, 13 cases demonstrated the punctuate pattern, and four showed nestin expression. In BC, 19 cases showed the punctuate pattern and 16 showed nestin expression. HPV-DNA integrates into the host genome, and this is observed as the punctuate pattern on ISH. The nestin expression was statistically high in group of BC than BD (P<0.01). These results therefore suggest that HPV-DNA integrated in the genome of tumor cells of these diseases and contributed to malignant alteration. From the standpoint of tumorigenesis, BC might represent one type of poorly differentiated, high-grade squamous cell carcinoma.
authors.affiliation: Department of Dermatology, Kitasato University School of Medicine, 1-15-1 Kitasato Minami, Sagamihara Kanagawa 252-0374, Japan. miisumi@med.kitasato-u.ac.jp
authors.forename: Sumiyuki
authors.initials: S
authors.lastname: Mii
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Amoh
authors.forename: Kenichi
authors.initials: K
authors.lastname: Tanabe
authors.forename: Hidero
authors.initials: H
authors.lastname: Kitasato
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Sato
authors.forename: Kensei
authors.initials: K
authors.lastname: Katsuoka
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001913:Bowen's Disease / Q000378:metabolism* / Q000821:virology*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D017403:In Situ Hybridization; D007381:Intermediate Filament Proteins / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D064231:Nestin; D027383:Papillomaviridae / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000378:metabolism* / Q000821:virology*; D012878:Skin Neoplasms / Q000378:metabolism* / Q000821:virology*; D014412:Tumor Virus Infections / Q000378:metabolism* / Q000821:virology*
chemical_list: D004279:DNA, Viral; D007381:Intermediate Filament Proteins; C577484:NES protein, human; D009419:Nerve Tissue Proteins; D064231:Nestin