title: Ultrasensitive DNA detection based on Au nanoparticles and isothermal circular double-assisted electrochemiluminescence signal amplification.
abstract: We report here an ultrasensitive DNA detection approach which combines Au NPs enhanced electrochemiluminescence (ECL) of the CdS nanocrystal (NC) film with isothermal circular amplification reaction of polymerase and nicking endonuclease (NEase). By the double-signal amplification, this approach could sensitively respond down to 5 aM DNA.
authors.affiliation: Key Lab of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, PR China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhou
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Jing-Juan
authors.initials: JJ
authors.lastname: Xu
authors.forename: Hong-Yuan
authors.initials: HY
authors.lastname: Chen
mesh_terms: D019187:Cadmium Compounds / Q000737:chemistry; D004247:DNA / Q000032:analysis*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D055664:Electrochemical Techniques; D004720:Endonucleases / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D008163:Luminescent Measurements / Q000379:methods*; D053768:Metal Nanoparticles / Q000737:chemistry*; D013440:Sulfides / Q000737:chemistry
chemical_list: D019187:Cadmium Compounds; D013440:Sulfides; C034939:cadmium sulfide; D006046:Gold; D004247:DNA; D004259:DNA-Directed DNA Polymerase; D004720:Endonucleases