title: Asiasari sieboldii suppresses inflammatory mediators through the induction of hemeoxygenase-1 expression in RAW264.7 cells.
abstract: OBJECTIVE
  Asiasari sieboldii is widely used in Korean traditional medicine. In this study, we examined the anti-inflammatory effects of A. sieboldii ethanolic extract (ASEE) in a lipopolysaccharide (LPS)-induced murine macrophage RAW264.7 cell, and then sought to understand the mechanism(s) underlying the observed effects.
  
  
  METHODS
  The production levels of nitrite oxide (NO), prostaglandin E₂ (PGE₂) and cytokines were measured using the Griess reagent and enzyme-linked immunosorbent assays (ELISA), while the cell protein expression levels were monitored by Western blot analysis.
  
  
  RESULTS
  Our results revealed that ASEE had prominent inhibitory effects on NO, PGE₂, interleukin (IL)-6 and tumor necrosis factor (TNF)-α production, as well as the expression of inducible nitrite oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), in LPS-induced RAW264.7 cells. Mechanistically, ASEE upregulated the expression of hemeoxygenase-1 (HO-1), and inhibited the nuclear translocation of nuclear factor (NF)-κB by preventing degradation of inhibitor κB-α (IκB-α).
  
  
  CONCLUSIONS
  These results indicate that the anti-inflammatory activity of ASEE occurs at least partially through the induction of HO-1 and subsequent suppression of the NF-κB pathway.
authors.affiliation: Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine, Jeonmin-dong, Yuseong-gu, Daejeon, Korea.
authors.forename: Jin-Ah
authors.initials: JA
authors.lastname: Lee
authors.forename: Mee-Young
authors.initials: MY
authors.lastname: Lee
authors.forename: Chang Seob
authors.initials: CS
authors.lastname: Seo
authors.forename: Hyekyung
authors.initials: H
authors.lastname: Ha
authors.forename: Hoyoung
authors.initials: H
authors.lastname: Lee
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Kim
authors.forename: Hyeun Kyoo
authors.initials: HK
authors.lastname: Shin
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology*; D029065:Aristolochiaceae / Q000737:chemistry*; D002460:Cell Line; D004790:Enzyme Induction / Q000187:drug effects; D051547:Heme Oxygenase-1 / Q000096:biosynthesis*; D020672:I-kappa B Proteins / Q000378:metabolism; D018836:Inflammation Mediators / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D055670:Medicine, Korean Traditional; D008565:Membrane Proteins / Q000096:biosynthesis*; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D000893:Anti-Inflammatory Agents; D020672:I-kappa B Proteins; D018836:Inflammation Mediators; D008565:Membrane Proteins; D016328:NF-kappa B; D010936:Plant Extracts; D051547:Heme Oxygenase-1; C496542:Hmox1 protein, mouse