title: [Effect of methylprednisolone pretreatment on pulmonary permeability and dipalmitoylphosphatidylcholine content in rabbits with reexpansion pulmonary edema].
abstract: OBJECTIVE
  To investigate the effects of methylprednisolone pretreatment on pulmonary lung permeability index and the content of the pulmonary surfactant dipalmitoylphosphatidylcholine (DPPC) in a rabbit model of reexpansion pulmonary edema.
  
  
  METHODS
  Twenty-one male New Zealand white rabbits were randomly divided into control group, reexpansion, and reexpansion+methylprednisolone pretreatment groups. The rabbit model of reexpansion pulmonary edema was established using Sakaos method. A bolus dosage of methylprednisolone (3 mg/kg) in reexpansion+methylprednisolone group group or 2.0 ml/kg normal saline in the other two groups was administered intravenously 20 min before reexpansion pulmonary edema. Bronchoalveolar lavage fluid (BALF) and arterial blood samples were collected for measurement of the total protein (TP) and DPPC contents 4 h after reexpansion, and the pulmonary permeability index was calculated.
  
  
  RESULTS
  The pulmonary permeability index in methylprednisolone pretreatment group was significantly lower than that in the reexpansion group (0.007∓0.002 vs 0.177∓0.004, P<0.05). Methylprednisolone pretreatment significantly increased DPPC concentration in the BALF as compared with saline treatment in the reexpansion group (61.815∓28.307 vs 101.955∓24.544 µg/ml, P<0.05).
  
  
  CONCLUSIONS
  Methylprednisolone pretreatment can increase pulmonary surfactant content and improve pulmonary permeability in the rabbit model of reexpansion pulmonary edema.
authors.affiliation: Department of Anesthesiology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. plentykyw@163.com
authors.forename: Yu-wen
authors.initials: YW
authors.lastname: Ke
authors.forename: Miao-ning
authors.initials: MN
authors.lastname: Gu
authors.forename: Qi-hong
authors.initials: QH
authors.lastname: Liu
authors.forename: Jian-she
authors.initials: JS
authors.lastname: Xu
authors.forename: Shan-he
authors.initials: SH
authors.lastname: Wan
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000032:analysis; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid; D002199:Capillary Permeability / Q000187:drug effects; D008297:Male; D008775:Methylprednisolone / Q000494:pharmacology*; D010539:Permeability; D011654:Pulmonary Edema / Q000378:metabolism* / Q000503:physiopathology*; D011663:Pulmonary Surfactants / Q000378:metabolism*; D011817:Rabbits
chemical_list: D011663:Pulmonary Surfactants; D015060:1,2-Dipalmitoylphosphatidylcholine; D008775:Methylprednisolone