title: CsrA and TnaB coregulate tryptophanase activity to promote exotoxin-induced killing of Caenorhabditis elegans by enteropathogenic Escherichia coli.
abstract: Enteropathogenic Escherichia coli(EPEC) requires the tnaA-encoded enzyme tryptophanase and its substrate tryptophan to synthesize diffusible exotoxins that kill the nematode Caenorhabditis elegans. Here, we demonstrate that the RNA-binding protein CsrA and the tryptophan permease TnaB coregulate tryptophanase activity, through mutually exclusive pathways, to stimulate toxin-mediated paralysis and killing of C. elegans.
authors.affiliation: Department of Pathology and Laboratory Medicine, Emory University, Atlanta, GA 30329, USA.
authors.forename: Shantanu
authors.initials: S
authors.lastname: Bhatt
authors.forename: Akwasi
authors.initials: A
authors.lastname: Anyanful
authors.forename: Daniel
authors.initials: D
authors.lastname: Kalman
mesh_terms: D000595:Amino Acid Sequence; D026905:Amino Acid Transport Systems / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000382:microbiology*; D054308:Enteropathogenic Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000472:pathogenicity*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D005098:Exotoxins / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D009876:Operon; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D014364:Tryptophan / Q000378:metabolism; D014368:Tryptophanase / Q000235:genetics* / Q000378:metabolism
chemical_list: D026905:Amino Acid Transport Systems; C093587:CsrA protein, E coli; D029968:Escherichia coli Proteins; D005098:Exotoxins; D016601:RNA-Binding Proteins; D012097:Repressor Proteins; C035131:TnaB protein, E coli; D014364:Tryptophan; D014368:Tryptophanase