title: Dynamic protein trafficking to the cell wall.
abstract: Recently we have studied the secretion pattern of a pectin methylesterase inhibitor protein (PMEI1) and a polygalacturonase inhibitor protein (PGIP2) in tobacco protoplast using the protein fusions, secGFP-PMEI1 and PGIP2-GFP. Both chimeras reach the cell wall by passing through the endomembrane system but using distinct mechanisms and through a pathway distinguishable from the default sorting of a secreted GFP. After reaching the apoplast, sec-GFP-PMEI1 is stably accumulated in the cell wall, while PGIP2-GFP undergoes endocytic trafficking. Here we describe the final localization of PGIP2-GFP in the vacuole, evidenced by co-localization with the marker Aleu-RFP, and show a graphic elaboration of its sorting pattern. A working model taking into consideration the presence of a regulated apoplast-targeted secretion pathway is proposed.
authors.affiliation: DiSTeBA, Università del Salento, Lecce, Italy.
authors.forename: Monica
authors.initials: M
authors.lastname: De Caroli
authors.forename: Marcello S
authors.initials: MS
authors.lastname: Lenucci
authors.forename: Gian-Pietro
authors.initials: GP
authors.lastname: Di Sansebastiano
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Dalessandro
authors.forename: Giulia
authors.initials: G
authors.lastname: De Lorenzo
authors.forename: Gabriella
authors.initials: G
authors.lastname: Piro
mesh_terms: D002473:Cell Wall / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008954:Models, Biological; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport / Q000235:genetics / Q000502:physiology*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins