title: SH2-containing inositol 5'-phosphatase inhibits transformation of Abelson murine leukemia virus.
abstract: v-Abl protein tyrosine kinase encoded by Abelson murine leukemia virus (Ab-MLV) transforms pre-B cells. Transformation requires the phosphatidylinositol 3-kinase (PI3K) pathway. This pathway is antagonized by SH2-containing inositol 5'-phosphatase (SHIP), raising the possibility that v-Abl modulates PI3K signaling through SHIP. Consistent with this, we show that v-Abl expression reduces levels of full-length p145 SHIP in a v-Abl kinase activity-dependent fashion. This event requires signals from the Abl SH2 domain but not the carboxyl terminus. Forced expression of full-length SHIP significantly reduces Ab-MLV pre-B-cell transformation. Therefore, reduction of SHIP protein by v-Abl is a critical component in Ab-MLV transformation.
authors.affiliation: Immunology Graduate Program, Sackler School of Graduate Biomedical Sciences, Tufts University School of Medicine, Boston, Massachusetts 02111, USA.
authors.forename: Shawn P
authors.initials: SP
authors.lastname: Fessler
authors.forename: Naomi
authors.initials: N
authors.lastname: Rosenberg
authors.forename: Linda B
authors.initials: LB
authors.lastname: Baughn
mesh_terms: D000011:Abelson murine leukemia virus / Q000472:pathogenicity*; D000818:Animals; D002472:Cell Transformation, Viral; D054884:Host-Pathogen Interactions; D000072181:Inositol Polyphosphate 5-Phosphatases; D051379:Mice; D016374:Oncogene Proteins v-abl / Q000378:metabolism*; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D054448:Precursor Cells, B-Lymphoid / Q000821:virology; D025941:Protein Interaction Mapping
chemical_list: D016374:Oncogene Proteins v-abl; D010744:Phosphoric Monoester Hydrolases; D000072181:Inositol Polyphosphate 5-Phosphatases