title: Lipid-induced peroxidation in the intestine is involved in glucose homeostasis imbalance in mice.
abstract: BACKGROUND
  Daily variations in lipid concentrations in both gut lumen and blood are detected by specific sensors located in the gastrointestinal tract and in specialized central areas. Deregulation of the lipid sensors could be partly involved in the dysfunction of glucose homeostasis. The study aimed at comparing the effect of Medialipid (ML) overload on insulin secretion and sensitivity when administered either through the intestine or the carotid artery in mice.
  
  
  RESULTS
  An indwelling intragastric or intracarotid catheter was installed in mice and ML or an isocaloric solution was infused over 24 hours. Glucose and insulin tolerance and vagus nerve activity were assessed. Some mice were treated daily for one week with the anti-lipid peroxidation agent aminoguanidine prior to the infusions and tests. The intestinal but not the intracarotid infusion of ML led to glucose and insulin intolerance when compared with controls. The intestinal ML overload induced lipid accumulation and increased lipid peroxidation as assessed by increased malondialdehyde production within both jejunum and duodenum. These effects were associated with the concomitant deregulation of vagus nerve. Administration of aminoguanidine protected against the effects of lipid overload and normalized glucose homeostasis and vagus nerve activity.
  
  
  CONCLUSIONS
  Lipid overload within the intestine led to deregulation of gastrointestinal lipid sensing that in turn impaired glucose homeostasis through changes in autonomic nervous system activity.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale, U1048, Toulouse, France.
authors.forename: Matteo
authors.initials: M
authors.lastname: Serino
authors.forename: Aurélie
authors.initials: A
authors.lastname: Waget
authors.forename: Nicolas
authors.initials: N
authors.lastname: Marsollier
authors.forename: Myriam
authors.initials: M
authors.lastname: Masseboeuf
authors.forename: Gaëlle
authors.initials: G
authors.lastname: Payros
authors.forename: Catherine
authors.initials: C
authors.lastname: Kabani
authors.forename: Jessica
authors.initials: J
authors.lastname: Denom
authors.forename: Amélie
authors.initials: A
authors.lastname: Lacombe
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Thiers
authors.forename: Anne
authors.initials: A
authors.lastname: Negre-Salvayre
authors.forename: Serge
authors.initials: S
authors.lastname: Luquet
authors.forename: Rémy
authors.initials: R
authors.lastname: Burcelin
authors.forename: Céline
authors.initials: C
authors.lastname: Cruciani-Guglielmacci
authors.forename: Christophe
authors.initials: C
authors.lastname: Magnan
mesh_terms: D000818:Animals; D005947:Glucose / Q000378:metabolism*; D006146:Guanidines / Q000494:pharmacology; D006706:Homeostasis; D007333:Insulin Resistance; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000187:drug effects*; D015227:Lipid Peroxidation / Q000187:drug effects; D051379:Mice; D018384:Oxidative Stress
chemical_list: D006146:Guanidines; D005947:Glucose; C004479:pimagedine