title: Temporal and spatial expression of cyclin H in rat spinal cord injury.
abstract: Cyclin H regulates cell cycle transitions; it always forms trimeric cyclin-dependent protein kinases (CDK)-activating kinase (CAK) complex with CDK7 and MAT1 that phosphorylates a threonine residue in the CDK2 T loop region. However, neither the expression nor function of cyclin H in the central nervous system (CNS) injury is still clear. Therefore, we studied cyclin H in a rat spinal cord contusion model. Injury markedly increased cyclin H protein expression throughout the thoracic spinal cord but did not increase CDK7. However, double immunofluorescent staining for proliferating cell nuclear antigen (PCNA) and cell markers revealed increases of cyclin H and CDK2 in proliferating microglia and astrocytes, and the co-immunoprecipitation studies shown that the associations of cyclin H with CDK2 were enhanced evidently after injury. Our data suggest that cyclin H may play a proliferative role in spinal cord injury (SCI).
authors.affiliation: Department of Orthopaedics, Affiliated Hospital of Nantong University, 226001, People's Republic of China.
authors.forename: Gang
authors.initials: G
authors.lastname: Wu
authors.forename: Jianhua
authors.initials: J
authors.lastname: Cao
authors.forename: Chen
authors.initials: C
authors.lastname: Peng
authors.forename: Huiguang
authors.initials: H
authors.lastname: Yang
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Cui
authors.forename: Jian
authors.initials: J
authors.lastname: Zhao
authors.forename: Qiyun
authors.initials: Q
authors.lastname: Wu
authors.forename: Jiangli
authors.initials: J
authors.lastname: Han
authors.forename: Honghui
authors.initials: H
authors.lastname: Li
authors.forename: Xingxing
authors.initials: X
authors.lastname: Gu
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D056748:Cyclin H / Q000235:genetics / Q000378:metabolism*; D051357:Cyclin-Dependent Kinase 2 / Q000378:metabolism; D018844:Cyclin-Dependent Kinases / Q000235:genetics / Q000378:metabolism; D008297:Male; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism* / Q000473:pathology; D013119:Spinal Cord Injuries / Q000378:metabolism* / Q000473:pathology; D013997:Time Factors; D000095043:Cyclin-Dependent Kinase-Activating Kinase
chemical_list: D015415:Biomarkers; D056748:Cyclin H; D018809:Proliferating Cell Nuclear Antigen; D051357:Cyclin-Dependent Kinase 2; D018844:Cyclin-Dependent Kinases; D000095043:Cyclin-Dependent Kinase-Activating Kinase