title: Vaccination with dengue virus-like particles induces humoral and cellular immune responses in mice.
abstract: BACKGROUND
  The incidence of dengue, an infectious disease caused by dengue virus (DENV), has dramatically increased around the world in recent decades and is becoming a severe public health threat. However, there is currently no specific treatment for dengue fever, and licensed vaccine against dengue is not available. Vaccination with virus-like particles (VLPs) has shown considerable promise for many viral diseases, but the effect of DENV VLPs to induce specific immune responses has not been adequately investigated.
  
  
  RESULTS
  By optimizing the expression plasmids, recombinant VLPs of four antigenically different DENV serotypes DENV1-4 were successfully produced in 293T cells. The vaccination effect of dengue VLPs in mice showed that monovalent VLPs of each serotype stimulated specific IgG responses and potent neutralizing antibodies against homotypic virus. Tetravalent VLPs efficiently enhanced specific IgG and neutralizing antibodies against all four serotypes of DENV. Moreover, vaccination with monovalent or tetravalent VLPs resulted in the induction of specific cytotoxic T cell responses.
  
  
  CONCLUSIONS
  Mammalian cell expressed dengue VLPs are capable to induce VLP-specific humoral and cellular immune responses in mice, and being a promising subunit vaccine candidate for prevention of dengue virus infection.
authors.affiliation: State Key Laboratory for Molecular Virology and Genetic Engineering, Institute for Viral Disease Control and Prevention, China CDC, 155 Chang Bai Road, Chang Ping District, Beijing 102206, China.
authors.forename: Shuo
authors.initials: S
authors.lastname: Zhang
authors.forename: Mifang
authors.initials: M
authors.lastname: Liang
authors.forename: Wen
authors.initials: W
authors.lastname: Gu
authors.forename: Chuan
authors.initials: C
authors.lastname: Li
authors.forename: Fang
authors.initials: F
authors.lastname: Miao
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Wang
authors.forename: Cong
authors.initials: C
authors.lastname: Jin
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Fushun
authors.initials: F
authors.lastname: Zhang
authors.forename: Quanfu
authors.initials: Q
authors.lastname: Zhang
authors.forename: Lifang
authors.initials: L
authors.lastname: Jiang
authors.forename: Mengfeng
authors.initials: M
authors.lastname: Li
authors.forename: Dexin
authors.initials: D
authors.lastname: Li
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood*; D002460:Cell Line; D053059:Dengue Vaccines / Q000008:administration & dosage* / Q000276:immunology*; D003716:Dengue Virus / Q000235:genetics; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014611:Vaccination / Q000379:methods; D022223:Vaccines, Subunit / Q000008:administration & dosage / Q000276:immunology; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology; D022721:Vaccines, Virosome / Q000008:administration & dosage / Q000276:immunology
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D053059:Dengue Vaccines; D007074:Immunoglobulin G; D022223:Vaccines, Subunit; D014614:Vaccines, Synthetic; D022721:Vaccines, Virosome