title: [Expression and clinical significance of COX-2 and BCL-2 in distal transitional mucosa adjacent to rectal carcinoma].
abstract: OBJECTIVE
  To investigate the expression of COX-2 and BCL-2 in transitional mucosa adjacent to rectal carcinoma, and to determine whether precursor event exists in the transitional mucosa.
  
  
  METHODS
  Mucin histochemical method (HID/AB) was used to determine the distal mucosa 2 cm away from rectal carcinoma in 54 patients with rectal cancer. Immunohistochemical method was employed to detect the expression of BCL-2 and COX-2 in the rectal cancer specimen, transitional mucosa (TM), non-transitional mucosa (NTM), and 20 cases of normal rectal mucosa. Student's t-test and Chi-square test were preformed.
  
  
  RESULTS
  Nineteen patients with positive TM were found. COX-2 expression was identified in 81.5% of cancer tissue, 21.1% of TM, 17.1% of NTM, and 10.0% in normal mucosa. BCL-2 protein was found in 77.8% of cancer tissue, 21.1% of TM, 22.9% of NTM, and 5.0% of normal mucosa. The expressions of COX-2 and BCL-2 in TM were significantly different from tumor tissue[(0.737±0.895) versus (3.519±1.998), and (0.632±0.955) versus (2.833±1.756), all P<0.01]. However, there were no significant differences between TM and NTM or normal mucosa.
  
  
  CONCLUSIONS
  Expressions of COX-2 and BCL-2 are non-specific in the transitional mucosa at the distal rectum. Evidence is not available in TM being precursor lesion.
authors.affiliation: Department of Coloproctology, Qingdao Municipal Hospital, Qingdao, China. zdfl2002@yahoo.com.cn
authors.forename: Dong-feng
authors.initials: DF
authors.lastname: Zhou
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.forename: Guo-dong
authors.initials: GD
authors.lastname: Pang
authors.forename: Yi-bo
authors.initials: YB
authors.lastname: Liang
authors.forename: Lin
authors.initials: L
authors.lastname: Cui
mesh_terms: D000328:Adult; D000368:Aged; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D005260:Female; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D012004:Rectal Neoplasms / Q000378:metabolism* / Q000473:pathology
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human