title: Role of the five RNA helicases in the adaptive response of Bacillus cereus ATCC 14579 cells to temperature, pH, and oxidative stresses.
abstract: In this study, growth rates and lag times of the five RNA helicase-deleted mutants of Bacillus cereus ATCC 14579 were compared to those of the wild-type strain under thermal, oxidative, and pH stresses. Deletion of cshD and cshE had no impact under any of the tested conditions. Deletion of cshA, cshB, and cshC abolished growth at 12°C, confirming previous results. In addition, we found that each RNA helicase had a role in a specific temperature range: deletion of cshA reduced growth at all the tested temperatures up to 45°C, deletion of cshB had impact below 30°C and over 37°C, and deletion of cshC led mainly to a cold-sensitive phenotype. Under oxidative conditions, deletion of cshB and cshC reduced growth rate and increased lag time, while deletion of cshA increased lag time only with H(2)O(2) and reduced growth rate at a high diamide concentration. Growth of the ΔcshA strain was affected at a basic pH independently of the temperature, while these conditions had a limited effect on ΔcshB and ΔcshC strain growth. The RNA helicases CshA, CshB, and CshC could participate in a general adaptation pathway to stressful conditions, with a stronger impact at low temperature and a wider role of CshA.
authors.affiliation: INRA, UMR408 Sécurité et Qualité des Produits d'Origine Végétale, Avignon, France.
authors.forename: Franck
authors.initials: F
authors.lastname: Pandiani
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Chamot
authors.forename: Julien
authors.initials: J
authors.lastname: Brillard
authors.forename: Frédéric
authors.initials: F
authors.lastname: Carlin
authors.forename: Christophe
authors.initials: C
authors.lastname: Nguyen-the
authors.forename: Véronique
authors.initials: V
authors.lastname: Broussolle
mesh_terms: D000222:Adaptation, Physiological; D001409:Bacillus cereus / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D003958:Diamide / Q000494:pharmacology; D017353:Gene Deletion; D005798:Genes, Bacterial; D006861:Hydrogen Peroxide / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D018384:Oxidative Stress; D020365:RNA Helicases / Q000235:genetics / Q000378:metabolism*; D013696:Temperature
chemical_list: D003958:Diamide; D006861:Hydrogen Peroxide; D020365:RNA Helicases