title: The fluorous effect in biomolecular applications.
abstract: From being a niche area only a few decades ago, fluorous chemistry has gained momentum and is, nowadays, a fervent area of research. It has brought forth, in fact, numerous applicative innovations that stretch among different fields: from catalysis to separation science, from supramolecular to materials and analytical chemistry. Recently, the unique features of perfluorinated compounds have reached the attention of the biochemists' audience. This tutorial review introduces the basic concepts of fluorous chemistry and illustrates its main biomolecular applications. Special attention has been given to fluorous microarrays and their combination with Mass-Spectroscopy (MS) techniques, to protein properties modification by the introduction of local fluorous domains, and to the most recent applications of (19)F-Magnetic Resonance Imaging ((19)F-MRI).
authors.affiliation: NFMLab-DCMIC Giulio Natta, Politecnico di Milano, via Mancinelli 7, I-20131 Milan, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Cametti
authors.forename: Benoit
authors.initials: B
authors.lastname: Crousse
authors.forename: Pierangelo
authors.initials: P
authors.lastname: Metrangolo
authors.forename: Roberto
authors.initials: R
authors.lastname: Milani
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Resnati
mesh_terms: D002241:Carbohydrates / Q000737:chemistry; D005461:Fluorine / Q000737:chemistry*; D008279:Magnetic Resonance Imaging; D013058:Mass Spectrometry; D046228:Microarray Analysis; D011506:Proteins / Q000737:chemistry
chemical_list: D002241:Carbohydrates; D011506:Proteins; D005461:Fluorine