title: Patterns of activities of root phosphomonoesterase and  phosphodiesterase in wetland plants as a function of  macrophyte species and ambient phosphorus regime.
abstract: Phosphorus (P)-limited plants produce higher amounts of root phosphatases, but research has mostly focused on phosphomonoesterases (PMEs). Because phosphate diesters can form a significant proportion of organic P in wetlands, we aimed to determine whether wetland plants produce both root PMEs and root phosphodiesterases (PDEs), and, if so, what factors influence activities of these enzymes. We measured the activities of root PMEs and PDEs colorimetrically in a wide range of macrophytes from natural and P-enriched wetlands. Hydrolyzable P in sediments was analyzed using commercially available PMEs and PDEs. In all species, both root PMEs and PDEs were always present, and their activities were closely correlated. Sedges and broadleaved emergents had the highest activity of both enzymes, while those of floating-leaved plants were the lowest. Redundancy analysis revealed close association between root enzymes and the proportion of monoesterase- and diesterase-hydrolyzable dissolved unreactive P. Both enzymes were positively correlated with root tissue N : P ratio. Both plant and sediment traits were important when explaining differences in enzyme activities. Although the activities are related to ambient P regime, the relationship was not close enough to use root enzymes as reliable predictors of dissolved unreactive P that is hydrolyzed by sediment phosphomono- and diesterases.
authors.affiliation: Department of Environmental Science and Policy, University of California Davis, One Shields Avenue, Davis 956 16, CA, USA.
authors.forename: Eliška
authors.initials: E
authors.lastname: Rejmánková
authors.affiliation: Department of Ecosystem Biology, Faculty of Science, University of South Bohemia Branišovská 31, České Budějovice 370 05, Czech Republic.
authors.forename: Dagmara
authors.initials: D
authors.lastname: Sirová
authors.affiliation: Department of Environmental Science and Policy, University of California Davis, One Shields Avenue, Davis 956 16, CA, USA.
authors.forename: Emily
authors.initials: E
authors.lastname: Carlson
mesh_terms: D001531:Belize; D009584:Nitrogen / Q000032:analysis / Q000378:metabolism; D010727:Phosphoric Diester Hydrolases / Q000032:analysis / Q000378:metabolism*; D010744:Phosphoric Monoester Hydrolases / Q000032:analysis / Q000378:metabolism*; D010758:Phosphorus / Q000032:analysis / Q000378:metabolism*; D018517:Plant Roots / Q000201:enzymology* / Q000378:metabolism; D018520:Plant Shoots / Q000201:enzymology* / Q000378:metabolism; D010944:Plants / Q000201:enzymology / Q000378:metabolism; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D053833:Wetlands
chemical_list: D012987:Soil; D010758:Phosphorus; D010744:Phosphoric Monoester Hydrolases; D010727:Phosphoric Diester Hydrolases; D009584:Nitrogen