title: Nitric oxide coordinates metabolism, growth, and development via the nuclear receptor E75.
abstract: Nitric oxide gas acts as a short-range signaling molecule in a vast array of important physiological processes, many of which include major changes in gene expression. How these genomic responses are induced, however, is poorly understood. Here, using genetic and chemical manipulations, we show that nitric oxide is produced in the Drosophila prothoracic gland, where it acts via the nuclear receptor ecdysone-induced protein 75 (E75), reversing its ability to interfere with its heterodimer partner, Drosophila hormone receptor 3 (DHR3). Manipulation of these interactions leads to gross alterations in feeding behavior, fat deposition, and developmental timing. These neuroendocrine interactions and consequences appear to be conserved in vertebrates.
authors.affiliation: Banting and Best Department of Medical Research, Department of Molecular Biology, University of Toronto, Ontario, Canada.
authors.forename: Lucía
authors.initials: L
authors.lastname: Cáceres
authors.forename: Aleksandar S
authors.initials: AS
authors.lastname: Necakov
authors.forename: Carol
authors.initials: C
authors.lastname: Schwartz
authors.forename: Sandra
authors.initials: S
authors.lastname: Kimber
authors.forename: Ian J H
authors.initials: IJ
authors.lastname: Roberts
authors.forename: Henry M
authors.initials: HM
authors.lastname: Krause
mesh_terms: D000818:Animals; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D004331:Drosophila melanogaster / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D004440:Ecdysone / Q000494:pharmacology; D005247:Feeding Behavior / Q000502:physiology; D016166:Free Radical Scavengers / Q000494:pharmacology; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D055785:Gene Knockdown Techniques; D007814:Larva; D050356:Lipid Metabolism; D008675:Metamorphosis, Biological / Q000235:genetics / Q000502:physiology; D009569:Nitric Oxide / Q000378:metabolism* / Q000494:pharmacology; D034622:RNA Interference; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism; D011987:Receptors, Steroid / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D016166:Free Radical Scavengers; C106186:Hr3 protein, Drosophila; D018160:Receptors, Cytoplasmic and Nuclear; D011987:Receptors, Steroid; D014157:Transcription Factors; C080885:nuclear hormone receptor FTZ-F1 beta, Drosophila; C061375:Eip75B protein, Drosophila; D009569:Nitric Oxide; D004440:Ecdysone