title: Cancer stem cell-like cells derived from malignant peripheral nerve sheath tumors.
abstract: This study aims to examine whether or not cancer stem cells exist in malignant peripheral nerve sheath tumors (MPNST). Cells of established lines, primary cultures and freshly dissected tumors were cultured in serum free conditions supplemented with epidermal and fibroblast growth factors. From one established human MPNST cell line, S462, cells meeting the criteria for cancer stem cells were isolated. Clonal spheres were obtained, which could be passaged multiple times. Enrichment of stem cell-like cells in these spheres was also supported by increased expression of stem cell markers such as CD133, Oct4, Nestin and NGFR, and decreased expression of mature cell markers such as CD90 and NCAM. Furthermore, cells of these clonal S462 spheres differentiated into Schwann cells, smooth muscle/fibroblast and neurons-like cells under specific differentiation-inducing cultural conditions. Finally, subcutaneous injection of the spheres into immunodeficient nude mice led to tumor formation at a higher rate compared to the parental adherent cells (66% versus 10% at 2.5 × 10(5)). These results provide evidence for the existence of cancer stem cell-like cells in malignant peripheral nerve sheath tumors.
authors.affiliation: Department of Maxillofacial Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
authors.forename: Melanie
authors.initials: M
authors.lastname: Spyra
authors.forename: Lan
authors.initials: L
authors.lastname: Kluwe
authors.forename: Christian
authors.initials: C
authors.lastname: Hagel
authors.forename: Rosa
authors.initials: R
authors.lastname: Nguyen
authors.forename: Jens
authors.initials: J
authors.lastname: Panse
authors.forename: Andreas
authors.initials: A
authors.lastname: Kurtz
authors.forename: Victor Felix
authors.initials: VF
authors.lastname: Mautner
authors.forename: Samuel David
authors.initials: SD
authors.lastname: Rabkin
authors.forename: Maria
authors.initials: M
authors.lastname: Demestre
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000235:genetics; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D048430:Cell Shape; D015536:Down-Regulation; D006801:Humans; D051379:Mice; D014411:Neoplastic Stem Cells / Q000378:metabolism / Q000473:pathology*; D018317:Nerve Sheath Neoplasms / Q000473:pathology*; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor