title: [Study of neutralization antibodie induced by DNA vaccine of HCV envelope protein 2 in mice].
abstract: OBJECTIVE
  To explore the feasibility of induction of neutralization antibodies against hepatitis C virus (HCV) infection by HCV envelope 2 protein (E2) DNA vaccines immunization.
  
  
  METHODS
  Two kinds of expression plasmids of HCV envelope 2 protein, plasmid pCI-1b661 Delta encoding hydrophobic carboxyl terminal truncated E2 and pCI-1b661 Delta encoding E2 with deletion of hypervariable region 1 (HVR1) and carboxyl terminal, were constructed and respectively transfeted 293T cells, and truncated E2 protein in whole cell lysate and supernatant of 293T cells were analyzed by Western blot. After BALB/c mouse were intramuscularly immunized by the plasmids, sera antibodies against HVR1 were detected by ELISA and the neutralization activity of the antibodies were assayed with HCV pseudotype particle (HCVpp).
  
  
  RESULTS
  Both plasmids could express secretary truncated E2 protein. All the mice immunized with plasmid pCI-1b661 produced HVR1 antibodies,while no HVR1 antibodies were detected in pCI-1b661 Delta immunized mice. The sera neutralization percentages against HCVpp in pCI1lb661 Delta and pCI-lb661 Delta immunized mice were (78.5 +/- 13.8)% and (38.7 +/- 6.5)%, respectively (P <0.01). Sera neutralization activity against HCVpp was positive correlated with the level of HVR1 antibodies in pCI-1b661 immunized mice (r = 0.967, P<0.01).
  
  
  CONCLUSIONS
  DNA vaccines expressing truncated E2 protein could induce neutralization antibodies against HCV, and neutralization antibodies mainly was consisted of the antibodies against HVR1.
authors.affiliation: Department of Microbiology, Medical School of Huzhou Teachers College, Huzhou 313000, China. shashe98@163.com
authors.forename: Shengwen
authors.initials: S
authors.lastname: Shao
authors.forename: Hongchang
authors.initials: H
authors.lastname: Zhou
authors.forename: Yimin
authors.initials: Y
authors.lastname: Tong
authors.forename: Yanli
authors.initials: Y
authors.lastname: Ren
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Chen
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood / Q000276:immunology*; D016174:Hepacivirus / Q000235:genetics / Q000276:immunology; D006508:Hepatitis Antibodies / Q000097:blood / Q000276:immunology*; D006526:Hepatitis C / Q000276:immunology / Q000517:prevention & control*; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D019444:Vaccines, DNA / Q000276:immunology; D014759:Viral Envelope Proteins / Q000276:immunology*; D014761:Viral Hepatitis Vaccines / Q000235:genetics / Q000276:immunology*; D014764:Viral Proteins / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; C400562:HVR1 protein, Hepatitis C virus; D006508:Hepatitis Antibodies; D019444:Vaccines, DNA; D014759:Viral Envelope Proteins; D014761:Viral Hepatitis Vaccines; D014764:Viral Proteins; C091015:glycoprotein E2, Hepatitis C virus