title: Anticoagulant, antioxidant and antitumor activities of heterofucans from the seaweed Dictyopteris delicatula.
abstract: In the present study, six families of sulfated polysaccharides were obtained from seaweed Dictyopteris delicatula by proteolytic digestion, followed by acetone fractionation and molecular sieving on Sephadex G-100. Chemical analyses demonstrated that all polysaccharides contain heterofucans composed mainly of fucose, xylose, glucose, galactose, uronic acid, and sulfate. The fucans F0.5v and F0.7v at 1.0 mg/mL showed high ferric chelating activity (∼45%), whereas fucans F1.3v (0.5 mg/mL) showed considerable reducing power, about 53.2% of the activity of vitamin C. The fucan F1.5v presented the most prominent anticoagulant activity. The best antiproliferative activity was found with fucans F1.3v and F0.7v. However, F1.3v activity was much higher than F0.7v inhibiting almost 100% of HeLa cell proliferation. These fucans have been selected for further studies on structural characterization as well as in vivo experiments, which are already in progress.
authors.affiliation: Laboratory of Biotechnology of Natural Polymers (BIOPOL), Department of Biochemistry, Federal University of Rio Grande do Norte (UFRN), 59020-100 Natal-RN, Brazil; E-Mails: kdmbio@yahoo.com.br (K.D.M.); leandro-silva-costa@hotmail.com (L.S.C.); gabrielfideliss@gmail.com (G.P.F.); rmo_85@hotmail.com (R.M.O.); leo_dnobre@yahoo.com.br (L.T.D.B.N.); nednaldod@hotmail.com (N.D.-S.); rafael_bgc@yahoo.com.br (R.B.G.C.); ivan.rui@click21.com.br (I.R.L.A.); sara-cordeiro@hotmail.com (S.L.C.); popoh_diego@hotmail.com (D.A.S.); marispc_bio@yahoo.com.br (M.S.S.P.C.); lucianagalves@hotmail.com (L.G.A.).
authors.forename: Kaline Dantas
authors.initials: KD
authors.lastname: Magalhaes
authors.forename: Leandro Silva
authors.initials: LS
authors.lastname: Costa
authors.forename: Gabriel Pereira
authors.initials: GP
authors.lastname: Fidelis
authors.forename: Ruth Medeiros
authors.initials: RM
authors.lastname: Oliveira
authors.forename: Leonardo Thiago Duarte Barreto
authors.initials: LT
authors.lastname: Nobre
authors.forename: Nednaldo
authors.initials: N
authors.lastname: Dantas-Santos
authors.forename: Rafael Barros Gomes
authors.initials: RB
authors.lastname: Camara
authors.forename: Ivan Rui Lopes
authors.initials: IR
authors.lastname: Albuquerque
authors.forename: Sara Lima
authors.initials: SL
authors.lastname: Cordeiro
authors.forename: Diego Araujo
authors.initials: DA
authors.lastname: Sabry
authors.forename: Mariana Santana Santos Pereira
authors.initials: MS
authors.lastname: Costa
authors.forename: Luciana Guimaraes
authors.initials: LG
authors.lastname: Alves
authors.forename: Hugo Alexandre Oliveira
authors.initials: HA
authors.lastname: Rocha
mesh_terms: D000925:Anticoagulants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D005663:Furans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D012624:Seaweed / Q000737:chemistry*
chemical_list: D000925:Anticoagulants; D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D005663:Furans; D010936:Plant Extracts