title: Ambidextrous magnetic nanovectors for synchronous gene transfection and labeling of human MSCs.
abstract: The synchronization of gene expression and cell trafficking in transfected stem cells is crucial for augmentation of stem cell functions (differentiation and neurotropic factor secretion) and real time in vivo monitoring. We report a magnetic nanoparticle-based gene delivery system that can ensure simultaneous gene delivery and in vivo cell trafficking by high resolution MR imaging. The polar aprotic solvent soluble MnFe₂O₄ nanoparticles were enveloped using cationic polymers (branched polyethyleneimine, PEI) by the solvent shifting method for a gene loading. Using our magnetic nanovector system (PEI-coated MnFe₂O₄ nanoparticles), thus, we synchronized stem cell migration and its gene expression in a rat stroke model.
authors.affiliation: Department of Radiology, Yonsei University College of Medicine, Seoul 120-752, Republic of Korea.
authors.forename: Jaemoon
authors.initials: J
authors.lastname: Yang
authors.forename: Eun-Sook
authors.initials: ES
authors.lastname: Lee
authors.forename: Min-Young
authors.initials: MY
authors.lastname: Noh
authors.forename: Seong-Ho
authors.initials: SH
authors.lastname: Koh
authors.forename: Eun-Kyung
authors.initials: EK
authors.lastname: Lim
authors.forename: A-Rum
authors.initials: AR
authors.lastname: Yoo
authors.forename: Kwangyeol
authors.initials: K
authors.lastname: Lee
authors.forename: Jin-Suck
authors.initials: JS
authors.lastname: Suh
authors.forename: Seung Hyun
authors.initials: SH
authors.lastname: Kim
authors.forename: Seungjoo
authors.initials: S
authors.lastname: Haam
authors.forename: Yong-Min
authors.initials: YM
authors.lastname: Huh
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005822:Genetic Vectors / Q000737:chemistry*; D006801:Humans; D007150:Immunohistochemistry; D008280:Magnetics; D059630:Mesenchymal Stem Cells / Q000737:chemistry / Q000378:metabolism*; D053758:Nanoparticles / Q000737:chemistry*; D051381:Rats; D017550:Spectroscopy, Fourier Transform Infrared; D014162:Transfection / Q000379:methods*