title: Evaluation of several adjuvants in avian influenza vaccine to chickens and ducks.
abstract: The effects of three different adjuvants, mineral oil, Montanide™ ISA 70M VG, and Montanide™ ISA 206 VG, were evaluated on reverse genetics H5N3 avian influenza virus cell cultured vaccine. The immune results of SPF chickens after challenging with highly pathogenic avian influenza (HPAI) virus demonstrated that mineral oil adjuvant group and 70M adjuvant group provided 100% protection efficiency, but 206 adjuvant group provided only 40%. Statistical analysis indicated that the protection effects of mineral oil adjuvant group and the 70M adjuvant showed no significant difference to each other, but with significant difference to 206 adjuvant group. All three groups could induce high titres of antibody after immunizing SPF ducks, but there was no significant difference among them. The immunization effect of 70M adjuvant group on SPF chickens were the best and showed significant difference compared with optimized 70Mi Montanide™ eight series adjuvants groups. These results suggest that 70M adjuvant could be a novel adjuvant for preparing avian influenza vaccine.
authors.affiliation: Animal Influenza Laboratory of Agriculture Ministry, National Avian Influenza Reference Laboratory, OIE Avian Influenza Reference Laboratory, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin, China.
authors.forename: Chun G
authors.initials: CG
authors.lastname: Liu
authors.forename: Ming
authors.initials: M
authors.lastname: Liu
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
authors.forename: Da F
authors.initials: DF
authors.lastname: Liu
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
authors.forename: Wei Q
authors.initials: WQ
authors.lastname: Pan
authors.forename: Hao
authors.initials: H
authors.lastname: Chen
authors.forename: Chun H
authors.initials: CH
authors.lastname: Wan
authors.forename: En C
authors.initials: EC
authors.lastname: Sun
authors.forename: Hong T
authors.initials: HT
authors.lastname: Li
authors.forename: Wen H
authors.initials: WH
authors.lastname: Xiang
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002645:Chickens; D002988:Cloaca / Q000821:virology; D053124:Influenza A Virus, H5N1 Subtype / Q000302:isolation & purification; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000276:immunology*; D005585:Influenza in Birds / Q000401:mortality / Q000473:pathology / Q000517:prevention & control*; D009821:Oils / Q000008:administration & dosage*; D010614:Pharynx / Q000821:virology; D016019:Survival Analysis
chemical_list: D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D007252:Influenza Vaccines; D009821:Oils