title: Isolation of genomic DNA using magnetic nanoparticles as a solid-phase support.
abstract: In recent years, techniques employing magnetizable solid-phase supports (MSPS) have found application in numerous biological fields. This magnetic separation procedure offers several advantages in terms of subjecting the analyte to very little mechanical stress compared to other methods. Secondly, these methods are non-laborious, cheap, and often highly scalable. The current paper details a genomic DNA isolation method optimized in our laboratory using magnetic nanoparticles as a solid-phase support. The quality and yields of the isolated DNA from all the samples using magnetic nanoparticles were higher or equivalent to the traditional DNA extraction procedures. Additionally, the magnetic method takes less than 15 min to extract polymerase chain reaction (PCR) ready genomic DNA as against several hours taken by traditional phenol-chloroform extraction protocols. Moreover, the isolated DNA was found to be compatible in PCR amplification and restriction endonuclease digestion. The developed procedure is quick, inexpensive, robust, and it does not require the use of organic solvents or sophisticated instruments, which makes it more amenable to automation and miniaturization.
authors.affiliation: Kemin Nutritional Technologies (India) Pvt Ltd, The Trapezium, Nelson Manickam Road, Chennai 600 029, India. Department of Biochemistry, Faculty of Science, The M S University of Baroda, Sayajigunj, Vadodara 390 002, India.
authors.forename: Z M
authors.initials: ZM
authors.lastname: Saiyed
authors.forename: C N
authors.initials: CN
authors.lastname: Ramchand
authors.forename: S D
authors.initials: SD
authors.lastname: Telang