title: Unusual growth variants of Avibacterium paragallinarum.
abstract: Two isolates of haemophilic bacteria originally isolated in the 1980s from chickens were re-examined. The addition of a 10% sterile filtrate from an overnight culture of Staphylococcus epidermidis allowed growth of both isolates in solid and liquid media that were otherwise not capable of supporting the growth of these isolates. Using the modified media, genotypic and serotypic studies were performed, which confirmed both isolates to be Avibacterium paragallinarum, with one isolate being serovar A and the other serovar C. The unusual growth requirements of these two isolates reinforces the need for careful interpretation by diagnostic laboratories examining chickens showing signs of upper respiratory tract disease.
authors.affiliation: Animal Research Institute, Agri-Science Queensland, DEEDI, Yeerongpilly, QLD, Australia. p.blackall@uq.edu.au
authors.forename: P J
authors.initials: PJ
authors.lastname: Blackall
authors.forename: H
authors.initials: H
authors.lastname: Christensen
authors.forename: M
authors.initials: M
authors.lastname: Bisgaard
mesh_terms: D000818:Animals; D002645:Chickens; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005838:Genotype; D006192:Haemophilus Infections / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D044134:Haemophilus paragallinarum / Q000254:growth & development / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011201:Poultry Diseases / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology*; D012703:Serotyping / Q000662:veterinary
chemical_list: D004269:DNA, Bacterial