title: Kinetics and differential expression of the skin-related chemokines CCL27 and CCL17 in psoriasis, atopic dermatitis and allergic contact dermatitis.
abstract: CCL27 and CCL17 are chemokines believed to be involved in the process of establishing the inflammatory infiltrate, characteristic for the various inflammatory skin diseases. The skin-specific CCL27 binds the chemokine receptor-10 (CCR10), and CCL17 is a chemokine receptor-4 (CCR4) ligand. The purpose of our study was to characterize the expression of CCL27 and CCL17 in the inflammatory skin diseases: psoriasis, atopic dermatitis (AD) and acute allergic contact dermatitis (ACD) induced in nickel-sensitive individuals. Surprisingly, our studies revealed a markedly decreased CCL27 mRNA and protein expression in psoriatic lesions compared with non-lesional psoriatic skin. A minor CCL17 mRNA increase was measured in lesional psoriatic skin. No alterations were found in AD. In ACD, we found a pronounced (90-fold) raise in CCL17 mRNA and a 50-fold increase in CCL17 protein compared with normal skin. A kinetic ACD study of CCL17 expression showed the highest mean value 24 h after hapten application. Furthermore, we found the mRNA levels of CCR10 and CCR4 paralleling the results of their corresponding ligands. Overall, our principal findings were a distinct decrease in CCL27 in lesional psoriatic skin and a marked upregulation of CCL17 in ACD. These findings underscore the differential cutaneous T-cell recruitment in different inflammatory diseases.
authors.affiliation: Department of Dermatology, Aarhus Sygehus, Aarhus University Hospital, Aarhus, Denmark. jette.lindorff.riis@ki.au.dk
authors.forename: Jette L
authors.initials: JL
authors.lastname: Riis
authors.forename: Claus
authors.initials: C
authors.lastname: Johansen
authors.forename: Christian
authors.initials: C
authors.lastname: Vestergaard
authors.forename: Rikke
authors.initials: R
authors.lastname: Bech
authors.forename: Knud
authors.initials: K
authors.lastname: Kragballe
authors.forename: Lars
authors.initials: L
authors.lastname: Iversen
mesh_terms: D000818:Animals; D054414:Chemokine CCL17 / Q000235:genetics* / Q000378:metabolism*; D054425:Chemokine CCL27 / Q000235:genetics* / Q000378:metabolism*; D018925:Chemokines / Q000235:genetics / Q000378:metabolism; D017449:Dermatitis, Allergic Contact / Q000209:etiology / Q000235:genetics* / Q000378:metabolism*; D003876:Dermatitis, Atopic / Q000235:genetics* / Q000378:metabolism*; D004195:Disease Models, Animal; D015870:Gene Expression; D006801:Humans; D007700:Kinetics; D051379:Mice; D008810:Mice, Inbred C57BL; D009532:Nickel / Q000009:adverse effects; D011565:Psoriasis / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: C106230:CCL17 protein, human; C492276:CCL27 protein, human; C499538:Ccl17 protein, mouse; D054414:Chemokine CCL17; D054425:Chemokine CCL27; D018925:Chemokines; D012333:RNA, Messenger; D009532:Nickel