title: A public genome-scale lentiviral expression library of human ORFs.
abstract: Functional characterization of the human genome requires tools for systematically modulating gene expression in both loss-of-function and gain-of-function experiments. We describe the production of a sequence-confirmed, clonal collection of over 16,100 human open-reading frames (ORFs) encoded in a versatile Gateway vector system. Using this ORFeome resource, we created a genome-scale expression collection in a lentiviral vector, thereby enabling both targeted experiments and high-throughput screens in diverse cell types.
authors.affiliation: RNA interference (RNAi) Platform, Broad Institute of Harvard and Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Yang
authors.forename: Jesse S
authors.initials: JS
authors.lastname: Boehm
authors.forename: Xinping
authors.initials: X
authors.lastname: Yang
authors.forename: Kourosh
authors.initials: K
authors.lastname: Salehi-Ashtiani
authors.forename: Tong
authors.initials: T
authors.lastname: Hao
authors.forename: Yun
authors.initials: Y
authors.lastname: Shen
authors.forename: Rakela
authors.initials: R
authors.lastname: Lubonja
authors.forename: Sapana R
authors.initials: SR
authors.lastname: Thomas
authors.forename: Ozan
authors.initials: O
authors.lastname: Alkan
authors.forename: Tashfeen
authors.initials: T
authors.lastname: Bhimdi
authors.forename: Thomas M
authors.initials: TM
authors.lastname: Green
authors.forename: Cory M
authors.initials: CM
authors.lastname: Johannessen
authors.forename: Serena J
authors.initials: SJ
authors.lastname: Silver
authors.forename: Cindy
authors.initials: C
authors.lastname: Nguyen
authors.forename: Ryan R
authors.initials: RR
authors.lastname: Murray
authors.forename: Haley
authors.initials: H
authors.lastname: Hieronymus
authors.forename: Dawit
authors.initials: D
authors.lastname: Balcha
authors.forename: Changyu
authors.initials: C
authors.lastname: Fan
authors.forename: Chenwei
authors.initials: C
authors.lastname: Lin
authors.forename: Lila
authors.initials: L
authors.lastname: Ghamsari
authors.forename: Marc
authors.initials: M
authors.lastname: Vidal
authors.forename: William C
authors.initials: WC
authors.lastname: Hahn
authors.forename: David E
authors.initials: DE
authors.lastname: Hill
authors.forename: David E
authors.initials: DE
authors.lastname: Root
mesh_terms: D003001:Cloning, Molecular / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics*; D015698:Genomic Library; D006801:Humans; D016086:Lentivirus / Q000235:genetics*; D016366:Open Reading Frames