title: Effects of vibration on the proteome expression of anterior cruciate ligament cells.
abstract: Recent reports have suggested that vibration has beneficial effects on knee healing response; however, the biomechanism of these beneficial effects still need to be determined on the anterior cruciate ligament (ACL) cell level. In this study, we applied a 20 Hz vibration to ACL cells, which produced a 20% increase (P < 0.001) in cell activity and 17% increase (P < 0.001) in intracellular sulfated glycosaminoglycan levels. In the 20 Hz vibration-stimulated ACL cell group, eight up-regulated (100 ∼ 300%) protein spots were identified compared with the control group by proteomics analysis. Among these proteins, Annexin A2 and Prolyl 4 hydroxylase (PH4B) were shown to have a 71% and 16% higher expression, respectively, in the 20 Hz vibration-stimulated ACL cell group by Western blotting (P < 0.001). These results indicate that vibration produces a positive cellular environment, and Annexin A2 and prolyl 4 hydroxylase are expected to help ligament repair and ACL cell proliferation by controlling cell membrane and extracellular matrix formation.
authors.affiliation: School of Life Sciences and Biotechnology, Korea University, 1-5 Anam Dong, Seongbuk-Gu, Seoul, Korea.
authors.forename: Gi-Yeon
authors.initials: GY
authors.lastname: Han
authors.forename: Seung-Ah
authors.initials: SA
authors.lastname: Park
authors.forename: Ji-Hye
authors.initials: JH
authors.lastname: Kim
authors.forename: Eun-Kyung
authors.initials: EK
authors.lastname: Lee
authors.forename: Hyun-Jung
authors.initials: HJ
authors.lastname: Kim
authors.forename: Young-Kwon
authors.initials: YK
authors.lastname: Seo
authors.forename: Jung-Keug
authors.initials: JK
authors.lastname: Park
authors.forename: Chan-Wha
authors.initials: CW
authors.lastname: Kim
mesh_terms: D016118:Anterior Cruciate Ligament / Q000737:chemistry* / Q000378:metabolism*; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D005786:Gene Expression Regulation; D020543:Proteome / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization; D013312:Stress, Physiological; D014732:Vibration
chemical_list: D020543:Proteome