title: Transplantation of neural stem cells overexpressing cardiotrophin-1 inhibits sprouting of hippocampal mossy fiber in a rat model of status epilepticus.
abstract: In this study, we studied the effects of hippocampal transplantation of neural stem cells (NSCs) overexpressing cardiotrophin 1 (CT1) on hippocampal mossy fiber sprouting (MFS) in a rat model of status epilepticus (SE). SE rats (lithium-pilocarpine model) were randomized into four study groups (18 rats per group): CT1-NSCs group, NSCs group, SE control group, and normal control group. Six rats were randomly chosen from each group at 1, 4, and 8 weeks after transplantation. MFS in hippocampal dentate gyrus was scored (Timm staining) at these time points. The MFS scores were as follows: CT1-NSCs 0.77 ± 0.04, 2.48 ± 0.89, and 2.39 ± 0.82 (1, 4, and 8 weeks after transplantation, respectively); NSCs 1.12 ± 0.62, 3.17 ± 0.64, and 3.88 ± 0.51; SE control 1.32 ± 0.35, 3.28 ± 0.75, and 4.32 ± 1.55; and normal control 0.37 ± 0.06, 0.34 ± 0.07, and 0.43 ± 0.04. Compared to SE control group and NSCs group, the scores of MFS in CT1-NSCs group were significantly lower (P < 0.05). In conclusion, transplantation with NSCs overexpressing CT1 inhibits hippocampal MFS and facilitates reduction of recurrent seizures.
authors.affiliation: Department of Pediatrics, First Affiliated Hospital, Zunyi Medical College, Zunyi, China. shuxiaomei1993@sina.com
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Shu
authors.forename: Shuzhen
authors.initials: S
authors.lastname: Du
authors.forename: Xuemei
authors.initials: X
authors.lastname: Chen
authors.forename: Zhenhong
authors.initials: Z
authors.lastname: Li
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D016207:Cytokines / Q000235:genetics*; D004195:Disease Models, Animal; D015870:Gene Expression; D008297:Male; D019599:Mossy Fibers, Hippocampal / Q000378:metabolism* / Q000473:pathology*; D058953:Neural Stem Cells / Q000637:transplantation*; D051381:Rats; D017208:Rats, Wistar; D013226:Status Epilepticus / Q000235:genetics* / Q000473:pathology* / Q000601:surgery / Q000628:therapy; D033581:Stem Cell Transplantation
chemical_list: D016207:Cytokines; C092073:cardiotrophin 1