title: Critical appraisal of published smear layer-removal studies: methodological issues.
abstract: The smear layer phenomenon has been extensively addressed over the past 3 decades. However, there still are unanswered questions on this issue and the main responsible factor is the qualitative and nonreproducible character of most in vitro smear layer removal studies. The methodological reasons that led to the current lack of reliable conclusions on procedures aiming to remove the smear layer are comprehensively addressed. The limitations and outcomes related to different assessment methods are discussed as well. General speaking, the results by score-based conventional SEM studies are not trustworthy and reproducible. Computational routines able to automatically extract quantitative data of dentin morphology are necessary to minimize the human bias. An ideal experimental model should have a longitudinal observational character, in which a given dentin area can be observed at different times. The use of flat dentin samples should be avoided because of the influence of gravity as well as root canal anatomy on the final result of the chelating process. The experimental model also needs to take into consideration the amount and distribution of sclerotic dentin and irregular secondary dentin, otherwise the final results can be compromised. Inasmuch as there is a clear lack of scientific standard, simple comparative SEM evaluations are pointless at the present moment. The ideal experimental model to assess smear layer removal is not currently available. Therefore, further research efforts should be directed to the development of and testing of new methodological approaches to be used in the smear layer removal studies. The improvement of the experimental models is a requirement to draw up optimized clinical guidelines for the chemical treatment of the root dentin.
authors.affiliation: UNIGRANRIO, School of Dentistry, Brazil. endogus@gmail.com
authors.forename: Gustavo
authors.initials: G
authors.lastname: De-Deus
authors.forename: Claudia
authors.initials: C
authors.lastname: Reis
authors.forename: Sidnei
authors.initials: S
authors.lastname: Paciornik
mesh_terms: D003786:Dental Pulp Cavity / Q000648:ultrastructure; D003804:Dentin / Q000648:ultrastructure; D006801:Humans; D008855:Microscopy, Electron, Scanning; D015203:Reproducibility of Results; D012107:Research Design / Q000592:standards; D012388:Root Canal Irrigants / Q000627:therapeutic use; D018915:Root Canal Preparation / Q000379:methods*; D016369:Smear Layer
chemical_list: D012388:Root Canal Irrigants