title: Twenty-four hour human urine and serum profiles of bisphenol a during high-dietary exposure.
abstract: By virtue of its binding to steroid hormone receptors, bisphenol A (BPA, the unconjugated bioactive monomer) is hypothesized to be estrogenic when present in sufficient quantities in the body, raising concerns that widespread exposure to BPA may impact human health. To better understand the internal exposure of adult humans to BPA and the relationship between the serum and urinary pharmacokinetics of BPA, a clinical exposure study was conducted. Blood and urine samples were collected approximately hourly over a 24-h period from 20 adult volunteers who ingested 100% of one of three specified meals comprising standard grocery store food items for breakfast, lunch, and dinner. The volunteers' average consumption of BPA, estimated from the urinary excretion of total BPA ((TOT)BPA = conjugated BPA + BPA), was 0.27 μg/kg body weight (range, 0.03-0.86), 21% greater than the 95th percentile of aggregate exposure in the adult U.S. population. A serum time course of (TOT)BPA was observable only in individuals with exposures 1.3-3.9 times higher than the 95th percentile of aggregate U.S. exposure. The (TOT)BPA urine concentration T(max) was 2.75 h (range, 0.75-5.75 h) post-meal, lagging the serum concentration T(max) by ∼1 h. Serum (TOT)BPA area under the curve per unit BPA exposure was between 21.5 and 79.0 nM•h•kg/μg BPA. Serum (TOT)BPA concentrations ranged from less than or equal to limit of detection (LOD, 1.3 nM) to 5.7 nM and were, on average, 42 times lower than urine concentrations. During these high dietary exposures, (TOT)BPA concentrations in serum were undetectable in 83% of the 320 samples collected and BPA concentrations were determined to be less than or equal to LOD in all samples.
authors.affiliation: Fundamental and Computational Sciences, Pacific Northwest National Laboratory, Richland, Washington 99352, USA. justin.teeguarden@pnl.gov
authors.forename: Justin G
authors.initials: JG
authors.lastname: Teeguarden
authors.forename: Antonia M
authors.initials: AM
authors.lastname: Calafat
authors.forename: Xiaoyum
authors.initials: X
authors.lastname: Ye
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Doerge
authors.forename: Mona I
authors.initials: MI
authors.lastname: Churchwell
authors.forename: Rudy
authors.initials: R
authors.lastname: Gunawan
authors.forename: Morgan K
authors.initials: MK
authors.lastname: Graham
mesh_terms: D000293:Adolescent; D000328:Adult; D001559:Benzhydryl Compounds; D003257:Consumer Product Safety; D004032:Diet; D004781:Environmental Exposure; D004784:Environmental Monitoring / Q000379:methods*; D004785:Environmental Pollutants / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D004968:Estrogens, Non-Steroidal / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D005260:Female; D005506:Food Contamination / Q000032:analysis*; D006801:Humans; D057230:Limit of Detection; D008297:Male; D008875:Middle Aged; D010636:Phenols / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D055815:Young Adult; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D004785:Environmental Pollutants; D004968:Estrogens, Non-Steroidal; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds