title: Bacteria-Induced Dscam Isoforms of the Crustacean, Pacifastacus leniusculus.
abstract: The Down syndrome cell adhesion molecule, also known as Dscam, is a member of the immunoglobulin super family. Dscam plays an essential function in neuronal wiring and appears to be involved in innate immune reactions in insects. The deduced amino acid sequence of Dscam in the crustacean Pacifastacus leniusculus (PlDscam), encodes 9(Ig)-4(FNIII)-(Ig)-2(FNIII)-TM and it has variable regions in the N-terminal half of Ig2 and Ig3 and the complete Ig7 and in the transmembrane domain. The cytoplasmic tail can generate multiple isoforms. PlDscam can generate more than 22,000 different unique isoforms. Bacteria and LPS injection enhanced the expression of PlDscam, but no response in expression occurred after a white spot syndrome virus (WSSV) infection or injection with peptidoglycans. Furthermore, PlDscam silencing did not have any effect on the replication of the WSSV. Bacterial specific isoforms of PlDscam were shown to have a specific binding property to each tested bacteria, E. coli or S. aureus. The bacteria specific isoforms of PlDscam were shown to be associated with bacterial clearance and phagocytosis in crayfish.
authors.affiliation: Department of Comparative Physiology, Uppsala University, Uppsala, Sweden.
authors.forename: Apiruck
authors.initials: A
authors.lastname: Watthanasurorot
authors.forename: Pikul
authors.initials: P
authors.lastname: Jiravanichpaisal
authors.forename: Haipeng
authors.initials: H
authors.lastname: Liu
authors.forename: Irene
authors.initials: I
authors.lastname: Söderhäll
authors.forename: Kenneth
authors.initials: K
authors.lastname: Söderhäll
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003400:Astacoidea; D001419:Bacteria / Q000276:immunology*; D015815:Cell Adhesion Molecules / Q000235:genetics*; D003445:Crustacea / Q000737:chemistry / Q000276:immunology / Q000382:microbiology*; D029721:Drosophila Proteins; D004926:Escherichia coli; D007136:Immunoglobulins; D007313:Insecta; D008969:Molecular Sequence Data; D010587:Phagocytosis; D020033:Protein Isoforms; D013211:Staphylococcus aureus
chemical_list: D015815:Cell Adhesion Molecules; D029721:Drosophila Proteins; C491736:Dscam1 protein, Drosophila; D007136:Immunoglobulins; D020033:Protein Isoforms