title: Lactococcus lactis as a live vector: heterologous protein production and DNA delivery systems.
abstract: Lactic acid bacteria (LAB), widely used in the food industry, are present in the intestine of most animals, including humans. The potential use of these bacteria as mucosal delivery vehicles for vaccinal, medical or technological use has been extensively investigated. Lactococcus lactis, a LAB species, is a potential candidate for the production of biologically useful proteins and for plasmid DNA delivery to eukaryotic cells. Several delivery systems have been developed to target heterologous proteins to a specific cell location (i.e., cytoplasm, cell wall or extracellular medium) and more recently to efficiently transfer DNA to eukaryotic cells. A promising application of L. lactis is its use for the development of live mucosal vaccines. Here, we have reviewed the expression of heterologous protein and the various delivery systems developed for L. lactis, as well as its use as an oral vaccine carrier.
authors.affiliation: Laboratório de Genética Celular e Molecular, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte-MG, Brazil.
authors.forename: Daniela Santos
authors.initials: DS
authors.lastname: Pontes
authors.forename: Marcela Santiago Pacheco
authors.initials: MS
authors.lastname: de Azevedo
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Chatel
authors.forename: Philippe
authors.initials: P
authors.lastname: Langella
authors.forename: Vasco
authors.initials: V
authors.lastname: Azevedo
authors.forename: Anderson
authors.initials: A
authors.lastname: Miyoshi
mesh_terms: D000818:Animals; D001424:Bacterial Infections / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000235:genetics* / Q000378:metabolism; D002473:Cell Wall / Q000235:genetics / Q000378:metabolism; D003600:Cytosol / Q000737:chemistry / Q000378:metabolism; D004247:DNA / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods*; D005110:Extracellular Space / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D015964:Gene Expression Regulation, Bacterial; D005822:Genetic Vectors / Q000008:administration & dosage / Q000627:therapeutic use*; D006801:Humans; D018928:Immunity, Mucosal; D007114:Immunization / Q000379:methods*; D013294:Lactococcus lactis / Q000235:genetics / Q000378:metabolism; D051379:Mice; D009092:Mucous Membrane / Q000276:immunology; D015202:Protein Engineering / Q000379:methods; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000235:genetics / Q000378:metabolism; D014777:Virus Diseases / Q000276:immunology / Q000517:prevention & control* / Q000821:virology
chemical_list: D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D019444:Vaccines, DNA; D004247:DNA