title: Position-dependent effect of a neural-restrictive silencer-like element present in the promoter downstream of the SCG10-like protein gene.
abstract: Neural-restrictive silencer (NRS) has been well characterized in SCG10 and many other neuron-specific genes; it is, however, unknown whether the promoters of the SCLIP and RB3 genes (two other SCG10 family members) share basal transcriptional mechanisms with SCG10 or not. To explore how NRS-mediated neural-specific gene transcription has evolved, we determined the genomic and promoter structures of the SCLIP gene, and found that the gene retained an NRS-like element that functioned as a negative regulator in non-neuronal cells. However, unlike the NRS in the SCG10 gene, this NRS(SCLIP) was located downstream of the transcription start site, and showed a position-dependent repressing activity. Further gel-shift and NRS factor (NRSF) co-transfection experiments revealed that NRS(SCLIP) was bound and regulated by NRSF. Such an element was not found in the gene promoter of RB3, suggesting that the NRS-NRSF regulatory system evolved once SCLIP had diverged from RB3 or stathmin.
authors.affiliation: Department of Molecular Genetics, National Institute for Longevity Sciences (NILS), Gengo 36-3, Morioka, Oobu, Aichi 474-8522, Japan.
authors.forename: Kiyoaki
authors.initials: K
authors.lastname: Sone
authors.forename: Masaaki
authors.initials: M
authors.lastname: Tsuda
authors.forename: Nozomu
authors.initials: N
authors.lastname: Mori
mesh_terms: D000818:Animals; D051379:Mice; D008969:Molecular Sequence Data; D009414:Nerve Growth Factors / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012097:Repressor Proteins / Q000378:metabolism*; D020218:Response Elements / Q000235:genetics*; D050777:Stathmin; D014158:Transcription, Genetic / Q000235:genetics; D000099229:RE1-Silencing Transcription Factor
chemical_list: D009414:Nerve Growth Factors; D012097:Repressor Proteins; D050777:Stathmin; D000099229:RE1-Silencing Transcription Factor; C491793:Stmn3 protein, mouse; C491794:Stmn4 protein, mouse