title: Detection of double stranded RNA in phytopathogenic Macrophomina phaseolina causing charcoal rot in Cyamopsis tetragonoloba.
abstract: One hundred one isolates of Macrophomina phaseolina from various hosts and eco-geographical locations were employed for elucidating relationships among genetic diversity and virulence. Highly pathogenic, moderately pathogenic, and hypovirulent cluster bean specific isolates were identified. In order to correlate respective phenotypes of plant pathogenic fungus multiple and complex patterns of dsRNA elements were analyzed. Double-stranded ribonucleic acids (dsRNA) are ubiquitous in all major groups and most of them have vast potential as biological control agents for fungi. Rate of virulence and its further association could ascertain by host plant and their fungal genotypes. Variability of the fungal genotypes decides the link between the complexity of dsRNA with different variants and the change in virulence pattern. Double-stranded RNA was identified in approximately 21.7% of M. phaseolina isolates from charcoal rot infected cluster bean varieties. After recurrent laboratory transfer on culture media, the preponderance of the isolates harboring dsRNAs developed degenerate culture phenotypes and showed reduced virulence (hypovirulence) to cluster bean. Macrophomina has successfully showed diversified and reproducible banding profile in dsRNA containing/free isolates. This is the first report of hypovirulence and detection of dsRNA in Macrophomina                         phaseolina isolates of cluster bean origin.
authors.affiliation: Department of Bio and Nano Technology, Guru Jambheshwar University of Science and Technology, Hisar, 125001 Haryana, India.
authors.forename: Pooja
authors.initials: P
authors.lastname: Arora
authors.forename: Neeraj
authors.initials: N
authors.lastname: Dilbaghi
authors.forename: Ashok
authors.initials: A
authors.lastname: Chaudhury
mesh_terms: D000704:Analysis of Variance; D001203:Ascomycota / Q000235:genetics* / Q000472:pathogenicity*; D031293:Cyamopsis / Q000382:microbiology*; D014644:Genetic Variation; D005838:Genotype; D007194:India; D010641:Phenotype; D012330:RNA, Double-Stranded / Q000235:genetics*; D014774:Virulence
chemical_list: D012330:RNA, Double-Stranded