title: The protective mechanism for the blood-brain barrier induced by aminoguanidine in surgical brain injury in rats.
abstract: This study was performed to investigate the mechanism of blood-brain barrier (BBB) permeability change, which was induced by aminoguanidine (AG) after surgical brain injury (SBI) in rats. Compared to control group, AG (150 mg/kg, i.p.) significantly reduced Evans blue extravasation into brain tissue at 24 h after surgical resection, it also induced a 32% decrease of malondialdehyde (MDA) values and a 1.1-fold increase of the glutathione (GSH) levels at 12 h after injury. The expression of inducible nitric oxide synthase (iNOS) reached the peak value at 24 h after SBI, which was significantly attenuated after AG treatment. In addition, ZO-1 protein was up-regulated by AG (150 mg/kg) treatment at 24 h after SBI. Our results indicated that AG could protect the BBB after SBI, which could be correlated with antioxidative property, the down-regulation of iNOS and up-regulation of tight junction protein expression.
authors.affiliation: Department of Anatomy, College Basic of Medical Sciences, China Medical University, Shenyang 110001, Liaoning Province, People's Republic of China.
authors.forename: Di
authors.initials: D
authors.lastname: Fan
authors.forename: Yan-ting
authors.initials: YT
authors.lastname: Gu
authors.forename: Hui
authors.initials: H
authors.lastname: Lv
authors.forename: Tao
authors.initials: T
authors.lastname: Tang
authors.forename: Zai-hua
authors.initials: ZH
authors.lastname: Xu
authors.forename: Zhen-quan
authors.initials: ZQ
authors.lastname: Song
authors.forename: Xiao-jie
authors.initials: XJ
authors.lastname: Tong
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier / Q000187:drug effects* / Q000502:physiology; D001930:Brain Injuries / Q000378:metabolism / Q000473:pathology*; D005978:Glutathione / Q000378:metabolism; D006146:Guanidines / Q000494:pharmacology*; D006801:Humans; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D010539:Permeability; D010750:Phosphoproteins / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D062826:Zonula Occludens-1 Protein
chemical_list: D006146:Guanidines; D008565:Membrane Proteins; D010750:Phosphoproteins; C569042:TJP1 protein, human; C569044:Tjp1 protein, rat; D062826:Zonula Occludens-1 Protein; D008315:Malondialdehyde; D052247:Nitric Oxide Synthase Type II; D005978:Glutathione; C004479:pimagedine