title: Decursin from Angelicagigas Nakai induces apoptosis in RC-58T/h/SA#4 primary human prostate cancer cells via a mitochondria-related caspase pathway.
abstract: Decursin is a major biological active component of Angelicagigas Nakai and is known to induce apoptosis of metastatic prostatic cancer cells. However, the apoptotic mechanism of decursin using primary malignant tumor (RC-58T/h/SA#4)-derived human prostate cells is not known. In the present study, we show that treatment of prostate cancer cells with decursin inhibited cell proliferation in a dose-dependent manner. Decursin also induced apoptosis in RC-58T/h/SA#4 cells, as determined by flow cytometry, Hoechst 33258 staining, and DNA fragmentation. Decursin caused activation of caspases-8, -9, and -3 and promoted the apoptotic action of caspase-8-mediated Bid cleavage. Decursin increased the protein levels of Bax and cytosolic cytochrome c as well as cleavage of PARP while decreasing the protein levels of Bcl-2. Furthermore, the caspase-independent mitochondrial apoptosis factor, apoptosis-inducing factor (AIF), was upregulated by treatment with decursin. Taken together, these findings indicate that decursin inhibited the proliferation of RC-58T/h/SA#4 cells through induction of apoptosis, which is mediated by both caspase-dependent and -independent apoptotic pathways.
authors.affiliation: Department of Food and Nutrition, Sunchon National University, Suncheon, Jeonnam 540-742, Republic of Korea.
authors.forename: Sa-Ra
authors.initials: SR
authors.lastname: Choi
authors.forename: Ju-Hye
authors.initials: JH
authors.lastname: Lee
authors.forename: Jae-Yong
authors.initials: JY
authors.lastname: Kim
authors.forename: Kyoung-Wuk
authors.initials: KW
authors.lastname: Park
authors.forename: Il-Yun
authors.initials: IY
authors.lastname: Jeong
authors.forename: Ki-Hwan
authors.initials: KH
authors.lastname: Shim
authors.forename: Mi-Kyung
authors.initials: MK
authors.lastname: Lee
authors.forename: Kwon-Il
authors.initials: KI
authors.lastname: Seo
mesh_terms: D029969:Angelica / Q000737:chemistry*; D017209:Apoptosis / Q000187:drug effects*; D001578:Benzopyrans / Q000494:pharmacology*; D006690:Bisbenzimidazole / Q000737:chemistry; D002087:Butyrates / Q000494:pharmacology*; D020169:Caspases / Q000378:metabolism*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D053938:DNA Fragmentation; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D008297:Male; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000187:drug effects / Q000201:enzymology; D009376:Neoplasms, Hormone-Dependent / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D018517:Plant Roots / Q000737:chemistry; D011471:Prostatic Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology
chemical_list: D001578:Benzopyrans; D002087:Butyrates; C101278:decursin; D020169:Caspases; D006690:Bisbenzimidazole