title: [Effect of protease in the lumen of rat intestine on inflammatory reaction during hemorrhagic shock].
abstract: OBJECTIVE
  To investigate the effect of intraluminal administration of ulinastatin (a protease inhibitor) in the intestine on intestinal inflammation in rats with hemorrhagic shock.
  
  
  METHODS
  Twenty-eight Wistar rats were randomized into control group (A), intestinal saline perfusion group (B), ulinastatin intestinal perfusion group (C), and intravenous ulinastatin injection group (D) (n=7). The mean arterial blood pressure (MAP) and survival time of the rats were recorded. The changes in human polymorphonuclear cell (PMN) CD11b expression were detected by flow cytometry. The leukocyte count was recorded at different time points after the treatment, and the pathology of the intestinal mucosa was observed comparatively.
  
  
  RESULTS
  Groups C and D showed significantly slower reduction of the MAP than groups A and B after hemorrhagic shock (P<0.05). The survival time of the rats was the longest in group C (P<0.05). CD11b expression increased gradually during hemorrhagic shock in all the groups, but the expression level was the lowest in group C (P<0.05). Hemorrhagic shock caused a reduction in leukocyte counts, which remained the highest in group C (P<0.05). Group C also showed the least intestinal pathology among the 4 groups.
  
  
  CONCLUSIONS
  Intestinal perfusion of ulinastatin can lower the reduction rate of MAP, attenuate plasma activation and intestinal inflammation, and prolong the survival of rats with hemorrhagic shock. These results indicate an important role of protease in intestinal inflammation during hemorrhagic shock.
authors.affiliation: Department of Surgery Intensive Care Unit, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. enma_01@163.com
authors.forename: Pei-pei
authors.initials: PP
authors.lastname: Guo
authors.forename: Zhong-qing
authors.initials: ZQ
authors.lastname: Chen
authors.forename: Xiao-li
authors.initials: XL
authors.lastname: Xi
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
authors.forename: Wei-jun
authors.initials: WJ
authors.lastname: Fu
authors.forename: Rui-ting
authors.initials: RT
authors.lastname: Wang
mesh_terms: D000818:Animals; D062186:Arterial Pressure; D004195:Disease Models, Animal; D006023:Glycoproteins / Q000008:administration & dosage / Q000494:pharmacology; D007249:Inflammation / Q000201:enzymology / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000201:enzymology*; D010949:Plasma / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012771:Shock, Hemorrhagic / Q000097:blood / Q000201:enzymology* / Q000378:metabolism; D014361:Trypsin Inhibitors / Q000008:administration & dosage / Q000494:pharmacology
chemical_list: D006023:Glycoproteins; D014361:Trypsin Inhibitors; C028665:urinastatin