title: Proteomic Analysis of Larval Midgut from the Silkworm (Bombyx mori).
abstract: The midgut is the major organ for food digestion, nutrient absorption and also a barrier for foreign substance. The 5th-instar larval stage of silkworm is very important for larval growth, development, and silk production. In the present study, we used 2-DE and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) to analyze the midgut proteins from the 5th-instar larvae as well as the midgut proteins under starvation condition. A total of 96 proteins were identified in this study; and among them, 69 proteins were observed in midgut for the first time. We also found that the silkworm larval midgut responded to starvation by producing a 10 kDa heat shock protein and a diapause hormone precursor.
authors.affiliation: The Key Sericultural Laboratory of Agricultural Ministry, College of Biotechnology, Southwest University, Beibei, Chongqing 400715, China.
authors.forename: Sai
authors.initials: S
authors.lastname: Zhang
authors.forename: Yunmin
authors.initials: Y
authors.lastname: Xu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Fu
authors.forename: Ling
authors.initials: L
authors.lastname: Jia
authors.forename: Zhonghuai
authors.initials: Z
authors.lastname: Xiang
authors.forename: Ningjia
authors.initials: N
authors.lastname: He