title: Improvement of the pilocarpine epilepsy model in rat using bone marrow stromal cell therapy.
abstract: OBJECTIVE
  Temporal lobe epilepsy is one of the most common types of epilepsy. Cytotherapy was tried for several neurological disorders. In this investigation, bone marrow stromal cells (BMSCs) were used in order to evaluate the recovery of epileptic rats induced by pilocarpine.
  
  
  METHODS
  The rats were divided into four groups: a negative control, treated groups, and epileptic rats treated with the vehicle only. The animals in the chronic phase were monitored for three weeks using Racine scale. BMSCs were labeled with BrdU and injected intravenously. After 6 weeks, the rats were killed and processed in paraffin as well as immunohistochemical techniques.
  
  
  RESULTS
  The results of the behavioral test showed that the number of seizures significantly decreased in treatment groups. Histopathology of the tissues from the untreated rats showed cell death and neurophagia. The numerical density of neurons per area was significantly higher in the treated groups than in the untreated ones. BMSCs localized in the hippocampus of the treated animals.
  
  
  CONCLUSIONS
  The results showed that there is structural and functional improvement in the epileptic rats treated with BMSCs.
authors.affiliation: Department of Anatomical Sciences, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Alireza
authors.initials: A
authors.lastname: Abdanipour
authors.forename: Taki
authors.initials: T
authors.lastname: Tiraihi
authors.forename: Javad
authors.initials: J
authors.lastname: Mirnajafi-Zadeh
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation / Q000379:methods*; D002478:Cells, Cultured; D004195:Disease Models, Animal; D004833:Epilepsy, Temporal Lobe / Q000139:chemically induced / Q000473:pathology / Q000601:surgery*; D008297:Male; D010862:Pilocarpine / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017154:Stromal Cells / Q000166:cytology / Q000637:transplantation
chemical_list: D010862:Pilocarpine