title: Green fluorescent protein: a perspective.
abstract: A brief personal perspective is provided for green fluorescent protein (GFP), covering the period 1994-2011. The topics discussed are primarily those in which my research group has made a contribution and include structure and function of the GFP polypeptide, the mechanism of fluorescence emission, excited state protein transfer, the design of ratiometric fluorescent protein biosensors and an overview of the fluorescent proteins derived from coral reef animals. Structure-function relationships in photoswitchable fluorescent proteins and nonfluorescent chromoproteins are also briefly covered.
authors.affiliation: Institute of Molecular Biology and Department of Physics, University of Oregon, Eugene, Oregon 97403-1229, USA. remington@molbio.uoregon.edu
authors.forename: S James
authors.initials: SJ
authors.lastname: Remington
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods; D005453:Fluorescence; D049452:Green Fluorescent Proteins / Q000737:chemistry*; D008164:Luminescent Proteins / Q000737:chemistry
chemical_list: D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins