title: Dicyanostilbene-derived two-photon fluorescence probe for lead ions in live cells and living tissues.
abstract: A novel two-photon fluorescence probe for Pb(2+) derived from 4-methyl-2,5-dicyano-4'-amino stilbene as a two-photon fluorophore and bis[2-(2-aminophenylsulfanyls)ethyl]amine as a novel Pb(2+) ligand was developed. The probe possesses small molecule size, large two-photon absorption cross-section (1020 GM), noncytotoxic effect, long-wavelength emission at 609 nm, large Stokes shift (209 nm), excellent photostability, moderate water-solubility, good cell-permeability, and pH-insensitivity in the biologically relevant pH range. The probe can selectively detect Pb(2+) ions in live cells and living tissues without interference from other metal ions and the membrane-bound probes, and its quenching constant (K(SV)(TP)) is 7.58×10(5) M(-1).
authors.affiliation: Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, China. huangchibao@163.com
authors.forename: Chibao
authors.initials: C
authors.lastname: Huang
authors.forename: Cairong
authors.initials: C
authors.lastname: Ding
mesh_terms: D000818:Animals; D001921:Brain / Q000473:pathology; D002460:Cell Line; D005456:Fluorescent Dyes / Q000737:chemistry*; D007259:Infrared Rays; D007477:Ions / Q000737:chemistry; D007854:Lead / Q000032:analysis*; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods*; D009570:Nitriles / Q000138:chemical synthesis / Q000737:chemistry*; D017785:Photons; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013267:Stilbenes / Q000737:chemistry*; D014753:Vinyl Compounds / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: C562129:2-(2-(4-(bis(2-(2-aminophenylsulfanyl) ethyl)amino)phenyl)vinyl)-5-methylterephthalonitrile; D005456:Fluorescent Dyes; D007477:Ions; D009570:Nitriles; D013267:Stilbenes; D014753:Vinyl Compounds; D007854:Lead