title: Molecular mechanism of silymarin-induced apoptosis in a highly metastatic lung cancer cell line anip973.
abstract: BACKGROUND
  Silymarin, the main flavonoid constituent element extracted from Silybum marianum possessing antioxidant activity, is already known to be able to block the NF-κB activation process and result in cell apoptosis, implicating silymarin's potential to control cancer cell growth.
  
  
  METHODS
  In this study, based upon the above assumption, silymarin was administered to a highly metastatic lung cancer cell line Anip973 to test silymarin's role in cancer cell proliferation.
  
  
  RESULTS
  Silymarin had significant inhibitory effects on the proliferation of Anip973 cells in a dose-dependent and time-response manner within 48 hours. Silymarin can induce Anip973 apoptosis.
  
  
  CONCLUSIONS
  Silymarin may in vitro inhibit the proliferation of the human lung adenocarcinoma cell line Anip973 and induce apoptosis via the mitochondria-dependent caspase cascade pathway.
authors.affiliation: Department of Thoracic Surgery, Tangdu Hospital, The Fourth Military Medical University, Xi'an, Shannxi, P.R. China.
authors.forename: Wenhai
authors.initials: W
authors.lastname: Li
authors.forename: Deguang
authors.initials: D
authors.lastname: Mu
authors.forename: Liqiang
authors.initials: L
authors.lastname: Song
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Liang
authors.forename: Chunmei
authors.initials: C
authors.lastname: Wang
authors.forename: Nannan
authors.initials: N
authors.lastname: Liu
authors.forename: Fei
authors.initials: F
authors.lastname: Tian
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Wang
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor / Q000379:methods; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy*; D008928:Mitochondria / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D009362:Neoplasm Metastasis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D012838:Silymarin / Q000494:pharmacology*; D013778:Tetrazolium Salts / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D000975:Antioxidants; D016328:NF-kappa B; D019253:Proto-Oncogene Proteins c-bcl-2; D012838:Silymarin; D013778:Tetrazolium Salts; D013844:Thiazoles; D051028:bcl-2-Associated X Protein; D053148:Caspase 3; D053453:Caspase 9; C022616:thiazolyl blue