title: Energy metabolism in human pluripotent stem cells and their differentiated counterparts.
abstract: BACKGROUND
  Human pluripotent stem cells have the ability to generate all cell types present in the adult organism, therefore harboring great potential for the in vitro study of differentiation and for the development of cell-based therapies. Nonetheless their use may prove challenging as incomplete differentiation of these cells might lead to tumoregenicity. Interestingly, many cancer types have been reported to display metabolic modifications with features that might be similar to stem cells. Understanding the metabolic properties of human pluripotent stem cells when compared to their differentiated counterparts can thus be of crucial importance. Furthermore recent data has stressed distinct features of different human pluripotent cells lines, namely when comparing embryo-derived human embryonic stem cells (hESCs) and induced pluripotent stem cells (IPSCs) reprogrammed from somatic cells.
  
  
  RESULTS
  We compared the energy metabolism of hESCs, IPSCs, and their somatic counterparts. Focusing on mitochondria, we tracked organelle localization and morphology. Furthermore we performed gene expression analysis of several pathways related to the glucose metabolism, including glycolysis, the pentose phosphate pathway and the tricarboxylic acid (TCA) cycle. In addition we determined oxygen consumption rates (OCR) using a metabolic extracellular flux analyzer, as well as total intracellular ATP levels by high performance liquid chromatography (HPLC). Finally we explored the expression of key proteins involved in the regulation of glucose metabolism.
  
  
  CONCLUSIONS
  Our results demonstrate that, although the metabolic signature of IPSCs is not identical to that of hESCs, nonetheless they cluster with hESCs rather than with their somatic counterparts. ATP levels, lactate production and OCR revealed that human pluripotent cells rely mostly on glycolysis to meet their energy demands. Furthermore, our work points to some of the strategies which human pluripotent stem cells may use to maintain high glycolytic rates, such as high levels of hexokinase II and inactive pyruvate dehydrogenase (PDH).
authors.affiliation: Pittsburgh Development Center, Magee Womens Research Institute, Pittsburgh, Pennsylvania, United States of America.
authors.forename: Sandra
authors.initials: S
authors.lastname: Varum
authors.forename: Ana S
authors.initials: AS
authors.lastname: Rodrigues
authors.forename: Michelle B
authors.initials: MB
authors.lastname: Moura
authors.forename: Olga
authors.initials: O
authors.lastname: Momcilovic
authors.forename: Charles A
authors.initials: CA
authors.lastname: Easley
authors.forename: João
authors.initials: J
authors.lastname: Ramalho-Santos
authors.forename: Bennett
authors.initials: B
authors.lastname: Van Houten
authors.forename: Gerald
authors.initials: G
authors.lastname: Schatten
mesh_terms: D015153:Blotting, Western; D002454:Cell Differentiation; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D004734:Energy Metabolism; D020869:Gene Expression Profiling; D006801:Humans; D007150:Immunohistochemistry; D008928:Mitochondria / Q000378:metabolism; D010085:Oxidative Phosphorylation; D010101:Oxygen Consumption; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014161:Transduction, Genetic