title: Generation and characterization of a monoclonal antibody against importin α7/NPI-2.
abstract: Many nuclear proteins are transported into the nucleus via the importin α/β-mediated pathway. Importin α comprises a multigene family. In this study, we generated and characterized a rat monoclonal antibody (MAb) 3F8 to importin α7. The antibody was generated by the hybridization of mouse myeloma cells with lymph node cells from an immunized rat. The MAb 3F8 specifically recognized importin α7 among importin α isoforms as evidenced by immunoblotting analysis. Furthermore, MAb 3F8 detected exogenous importin α7 in COS-7 cells by immunofluorescence. This MAb will be useful in the analysis of the isoform-specific function of importin α7.
authors.affiliation: Biomolecular Dynamics Group, Department of Frontier Biosciences, Graduate School of Frontier Biosciences, Osaka, Japan.
authors.forename: Chiaki
authors.initials: C
authors.lastname: Mizuguchi
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Moriyama
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Yoneda
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology*; D019556:COS Cells; D045744:Cell Line, Tumor; D002522:Chlorocebus aethiops; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006825:Hybridomas / Q000276:immunology*; D015151:Immunoblotting; D051379:Mice; D051381:Rats; D028901:alpha Karyopherins / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; C423182:Kpna6 protein, rat; D028901:alpha Karyopherins