title: Polyphenolics profile and antioxidant properties of Raphanus sativus L.
abstract: Raphanus sativus, a common cruciferous vegetable has been attributed to possess a number of pharmacological properties. Antioxidant and radical scavenging activity of R. sativus root extracted with solvents of varying polarity were evaluated using different model systems. Polyphenolic content was estimated to be in the range 13.18-63.54 mg g⁻¹ dry weight, with a considerable amount being obtained with polar solvents. High-performance liquid chromatography analysis indicated the presence of an array of polyphenolics. Catechin was found to be the most abundant phenolic compound in water extract and sinapic acid, the predominant phenolic compound in methanolic, ethyl acetate and hexane extracts. The methanolic extract showed significant ferric reducing ability, moderate metal chelating activity and strong radical scavenging activity. The methanolic extract could be successfully utilised as an ingredient in functional foods. However, water extract could be more pertinent to human nutrition as it contained a significant amount of catechin, which was comparable to traditional sources like green and black tea.
authors.affiliation: Centre for Biotechnology, Institute of Science and Technology, Jawaharlal Nehru Technological University, Kukatpally, Hyderabad 500085 Andhra Pradesh, India.
authors.forename: Syed Sultan
authors.initials: SS
authors.lastname: Beevi
authors.forename: Lakshmi Narasu
authors.initials: LN
authors.lastname: Mangamoori
authors.forename: Bandi Boje
authors.initials: BB
authors.lastname: Gowda
mesh_terms: D000975:Antioxidants / Q000032:analysis*; D002614:Chelating Agents / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D016166:Free Radical Scavengers / Q000032:analysis; D007501:Iron / Q000737:chemistry; D019787:Linoleic Acid / Q000737:chemistry; D015227:Lipid Peroxidation; D018517:Plant Roots / Q000737:chemistry*; D059808:Polyphenols / Q000032:analysis*; D031224:Raphanus / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D002614:Chelating Agents; D016166:Free Radical Scavengers; D059808:Polyphenols; D019787:Linoleic Acid; D007501:Iron