title: Gene expression profiling supports the role of Repin1 in the pathophysiology of metabolic syndrome.
abstract: Congenic BB rat strains carrying a SHR segment (D4Got41-Tacr1; 60.5-122.8 Mb; BB.4S) or a WOKW segment (D4Got41-Fabp1; 60.5-104.6 Mb; BB.4W) of chromosome 4 within the BB/OK background develop facets of the metabolic syndrome when compared with their parental BB/OK rats. To narrow down potential genes involved in the pathophysiology of metabolic syndrome, gene expression studies in adipose tissues of BB/OK, BB.4S, and BB.4W rats were initiated. Total RNA of subcutaneous and epididymal adipose tissue of BB/OK (n=10), congenic BB.4S (n=8), and BB.4W (n=9) males at an age of 4 weeks was isolated. The mRNA expression of 92 genes involved in obesity, insulin resistance and other metabolic traits was measured by RT-PCR. Significant differences in gene expression were only found in Repin1 in both adipose tissues. Congenic BB.4W showed significantly lower gene expression than did BB.4S and BB/OK. Our findings and newly published findings of Repin1 in 3T3-L1 adipocytes support the hypothesis that Repin1 may affect the development of facets of the metabolic syndrome.
authors.forename: Jeanette
authors.initials: J
authors.lastname: Bahr
authors.forename: Nora
authors.initials: N
authors.lastname: Klöting
authors.forename: Ingrid
authors.initials: I
authors.lastname: Klöting
authors.forename: Niels
authors.initials: N
authors.lastname: Follak
mesh_terms: D052436:Adipose Tissue, White / Q000378:metabolism*; D000818:Animals; D020296:Animals, Congenic; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004195:Disease Models, Animal; D004822:Epididymis; D050556:Fatty Acid-Binding Proteins / Q000235:genetics; D020869:Gene Expression Profiling; D008297:Male; D024821:Metabolic Syndrome / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011913:Rats, Inbred BB; D018040:Receptors, Neurokinin-1 / Q000235:genetics; D013047:Specific Pathogen-Free Organisms; D050151:Subcutaneous Fat / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D004268:DNA-Binding Proteins; C493321:Fabp1 protein, rat; D050556:Fatty Acid-Binding Proteins; D012333:RNA, Messenger; D018040:Receptors, Neurokinin-1; C522919:Repin1 protein, rat