title: Susceptibility of Candida albicans and Candida dubliniensis to erythrosine- and LED-mediated photodynamic therapy.
abstract: The effect of erythrosine- and LED-mediated photodynamic therapy (PDT) on planktonic cultures and biofilms of Candida albicans and Candida dubliniensis was evaluated. Planktonic cultures of standardized suspensions (10(6)cells/mL) of C. albicans and C. dubliniensis were treated with erythrosine concentrations of 0.39-200 μM and LEDs in a 96-well microtiter plate. Biofilms formed by C. albicans and C. dubliniensis in the bottom of a 96-well microtiter plate were treated with 400 μM erythrosine and LEDs. After PDT, the biofilms were analysed by scanning electron microscopy (SEM). The antimicrobial effect of PDT against planktonic cultures and biofilms was verified by counting colony-forming units (CFU/mL), and the data were submitted to analysis of variance and the Tukey test (P<0.05). C. albicans and C. dubliniensis were not detectable after PDT of planktonic cultures with erythrosine concentrations of 3.12 μM or higher. The CFU/mL values obtained from biofilms were reduced 0.74 log(10) for C. albicans and 0.21 log(10) for C. dubliniensis. SEM revealed a decrease in the quantity of yeasts and hyphae in the biofilm after PDT. In conclusion, C. albicans and C. dubliniensis were susceptible to erythrosine- and LED-mediated PDT, but the biofilms of both Candida species were more resistant than their planktonic counterparts.
authors.affiliation: School of Dentistry of São José dos Campos, Department of Biosciences and Oral Diagnosis, UNESP - Univ Estadual Paulista, Engenheiro Francisco José Longo, 777, Jardim São Dimas, São José dos Campos, SP, CEP: 12245-000, Brazil. carol_biolog@yahoo.com.br
authors.forename: Anna Carolina Borges Pereira
authors.initials: AC
authors.lastname: Costa
authors.forename: Vanessa Maria
authors.initials: VM
authors.lastname: de Campos Rasteiro
authors.forename: Cristiane Aparecida
authors.initials: CA
authors.lastname: Pereira
authors.forename: Emily Setsuko Halter
authors.initials: ES
authors.lastname: da Silva Hashimoto
authors.forename: Milton
authors.initials: M
authors.lastname: Beltrame
authors.forename: Juliana Campos
authors.initials: JC
authors.lastname: Junqueira
authors.forename: Antonio Olavo Cardoso
authors.initials: AO
authors.lastname: Jorge
mesh_terms: D018441:Biofilms / Q000187:drug effects*; D002175:Candida / Q000145:classification / Q000187:drug effects*; D002176:Candida albicans / Q000187:drug effects; D002180:Candidiasis, Oral / Q000188:drug therapy; D015169:Colony Count, Microbial; D003116:Color; D025141:Drug Resistance, Fungal; D004923:Erythrosine / Q000494:pharmacology* / Q000627:therapeutic use; D005456:Fluorescent Dyes / Q000494:pharmacology* / Q000627:therapeutic use; D008826:Microbial Sensitivity Tests; D008855:Microscopy, Electron, Scanning; D010778:Photochemotherapy; D010933:Plankton / Q000187:drug effects; D012666:Semiconductors
chemical_list: D005456:Fluorescent Dyes; D004923:Erythrosine