title: IL-10 overexpression attenuated expression of TNF-α and IL-1β activated by lipopolysaccharide in astrocytes.
abstract: OBJECTIVE
  To investigate the intervention effect of lentivirus expressing human IL-10 (LV-hIL-10) on activated astrocytes.
  
  
  METHODS
  DI TNC1 cell line was treated with different concentrations of lipopolysaccharide (LPS) and time points. The expression of proinflammatory cytokines tumor necrosis factor-α (TNF-α) and interleukinb-1β (IL-1β) was detected by RT-PCR and ELISA. Moreover, the effect on the expression of proinflammatory cytokines TNF-α and IL-1β was analyzed in DI TNC1 cell lines infected with and without LV-hIL-10.
  
  
  RESULTS
  The expression of TNF-α and IL-1β was increased in DI TNC1 induced by LPS. The expression of IL-10 was upregulated in DI TNC1 infected with LV-hIL-10. TNF-α and IL-1β were inhibited by IL-10 overexpression in DI TNC1 actived by LPS.
  
  
  CONCLUSIONS
  DI TNC1 is activated by LPS and secretes proinflammatory cytokines TNF-α and IL-1β as immune-like cells, and these activation is inhibited by hIL-10 overexpression.
authors.affiliation: Department of Anesthesiology, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Zhenghua
authors.initials: Z
authors.lastname: He
authors.forename: Muzhang
authors.initials: M
authors.lastname: Xiao
authors.forename: Qulian
authors.initials: Q
authors.lastname: Guo
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000378:metabolism*; D001921:Brain / Q000166:cytology; D002460:Cell Line; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics / Q000378:metabolism*; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D051381:Rats; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*
chemical_list: D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10