title: A Micellar Complex of a Conjugated Polyelectrolyte for Efficient FRET to Dye-Labeled DNA.
abstract: A polymer-surfactant micellar complex has been studied as a fluorescence resonance energy transfer (FRET) donor to fluorescein-labeled DNA (ssDNA-Fl). In water, the molar absorptivity and fluorescence quantum efficiency of cationic poly(fluorene-co-phenylene) (c-PFP) are substantially increased in the presence of non-ionic surfactants. A TEM microscopic study shows the formation of a nanowire micellar complex of c-PFP and the surfactants. About a 400% enhancement of the FRET signal is measured in c-PFP/ssDNA-Fl with Brij 30, relative to that without surfactants. The signal amplification is successfully modulated using different types of non-ionic surfactants which perturb the complexation, fine-structure of the complex (i.e., donor-acceptor separation), and the resulting energy transfer process.
authors.affiliation: Department of Nanomaterials Engineering (BK21), Pusan National University, Miryang 627-706, South Korea.
authors.forename: Rati Ranjan
authors.initials: RR
authors.lastname: Nayak
authors.forename: Okhil Kumar
authors.initials: OK
authors.lastname: Nag
authors.forename: Mijeong
authors.initials: M
authors.lastname: Kang
authors.forename: Youngeup
authors.initials: Y
authors.lastname: Jin
authors.forename: Hongsuk
authors.initials: H
authors.lastname: Suh
authors.forename: Kwanghee
authors.initials: K
authors.lastname: Lee
authors.forename: Han Young
authors.initials: HY
authors.lastname: Woo