title: [Simultaneous quantitation of adonifoline and senecionine in Senecio herbs by UPLC-MS].
abstract: OBJECTIVE
  To develop an UPLC-MS method for the simultaneously quantitation of adonifoline and senecinine in Senecio herbs.
  
  
  METHODS
  UPLC-Micro 2000 was used for quantification in SIR mode under ESI+. Monocrotaline was used as the internal standard. Chromatography was performed on a Shiseido Capcell Pak MG (2.0 mm x 50 mm, 3 microm) column at 30 degrees C using an gradient elution of acetonitrile-0.5% formic acid in water at the flow rate of 0.3 mL x min(-1). The injection volume was 2 microL.
  
  
  RESULTS
  Good linearity was obtained for quantitation of adonifoline over the range of 1.02-816.00 microg x L(-1) (r = 0.998 0). And recoveries at different concentration levels were between 95.73% and 103.0% with RSDs no more than 2.5%. For quantification of senecionine, the linear range was between 1.08-860.56 microg x L(-1) (r = 0.997 6). And recoveries at different concentration levels were between 95.67% and 101.5% with RSDs no more than 2.3%. Good reproducibility and precision were also achieved.
  
  
  CONCLUSIONS
  The new developed UPLC method is sensitive, accurate and reliable enough for the quantitation of adonifoline and senecionine in Senecio herbs thus can be used for the limit detection of pyrrolizidne alkaloids in S. scandens. It can also be used for the identification of fake drugs of S. scandens such as S. vulgaris. The developed method was served for the quality evaluation of Herba Senecionis Scandentis.
authors.affiliation: MOE Key Laboratory for Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors.forename: Aizhen
authors.initials: A
authors.lastname: Xiong
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.forename: Xuejing
authors.initials: X
authors.lastname: Yang
authors.forename: Changhong
authors.initials: C
authors.lastname: Wang
authors.forename: Zhengtao
authors.initials: Z
authors.lastname: Wang
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D007783:Lactones / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*; D011763:Pyrrolizidine Alkaloids / Q000032:analysis*; D012674:Senecio / Q000737:chemistry*
chemical_list: D007783:Lactones; D011763:Pyrrolizidine Alkaloids; C546218:adonifoline; C009237:senecionine