title: Protein kinase C beta in postischemic brain mitochondria.
abstract: PKC is implicated in the regulation of mitochondrial metabolism. We examined the association of PKCβ with mitochondria and followed postischemic changes in its amount in mitochondria isolated from ischemia-vulnerable (CA1) and ischemia-resistant (CA2-4,DG) hippocampus in gerbil model of transient brain ischemia. Our observations suggest that transient ischemic episode induces a significant, rapid and long lasting increase of PKCβ in mitochondria in CA2-4,DG, which may bespeak neuroprotection. In organotypic hippocampal culture (OHC) model of neurodegeneration, PKCβ inhibition imposed over NMDA toxicity extended the death area beyond the CA1. These results suggest that PKCβ might have a protective effect against excitotoxic damage in rat OHC. The pull-down method and LC-MS/MS analysis revealed mitochondrial proteins that can bind directly with PKCβΙ. The proteins were parts of i) mitochondrial redox carriers forming the electron transport chain including ATP synthase and ii) MPTP: ANT and creatine kinase. PKCβ acting through mitochondrial proteins could play a role in protecting the cells from death by e.g. influencing ROS and ATP production after ischemia in CA2-4,DG region of the hippocampus.
authors.affiliation: Molecular Biology Unit, Mossakowski Medical Research Centre, Polish Academy of Sciences, 5 Pawińskiego St., 02-106 Warsaw, Poland.
authors.forename: Joanna E
authors.initials: JE
authors.lastname: Kowalczyk
authors.forename: Maria
authors.initials: M
authors.lastname: Kawalec
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Beręsewicz
authors.forename: Janusz
authors.initials: J
authors.lastname: Dębski
authors.forename: Michał
authors.initials: M
authors.lastname: Dadlez
authors.forename: Barbara
authors.initials: B
authors.lastname: Zabłocka
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000503:physiopathology*; D005849:Gerbillinae; D006624:Hippocampus / Q000201:enzymology*; D008928:Mitochondria / Q000201:enzymology*; D011493:Protein Kinase C / Q000378:metabolism*; D064546:Protein Kinase C beta; D051381:Rats; D017208:Rats, Wistar
chemical_list: D011493:Protein Kinase C; D064546:Protein Kinase C beta