title: The development and characterization of protein-based stationary phases for studying drug-protein and protein-protein interactions.
abstract: Protein-based liquid chromatography stationary phases are used in bioaffinity chromatography for studying drug-protein interactions, the determination of binding affinities, competitive and allosteric interactions, as well as for studying protein-protein interactions. This review addresses the development and characterization of protein-based stationary phase, and the application of these phases using frontal and zonal chromatography techniques. The approach will be illustrated using immobilized heat shock protein 90α and the immobilized estrogen related receptor stationary phases. In addition, the review discusses the use of the protein-coated magnetic beads for ligand and protein fishing as well as for the identification of unknown ligands from cellular or botanical extracts.
authors.affiliation: Gerontology Research Center, National institute on Aging, National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, MD 21224, USA.
authors.forename: Mitesh
authors.initials: M
authors.lastname: Sanghvi
authors.forename: Ruin
authors.initials: R
authors.lastname: Moaddel
authors.forename: Irving W
authors.initials: IW
authors.lastname: Wainer
mesh_terms: D002846:Chromatography, Affinity / Q000379:methods*; D055808:Drug Discovery; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry* / Q000378:metabolism; D004364:Pharmaceutical Preparations / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding
chemical_list: D055767:Immobilized Proteins; D004364:Pharmaceutical Preparations