title: [Rapid analysis of L-carnitine in health food by high performance anion exchange chromatography with pulsed amperometric detector].
abstract: OBJECTIVE
  A pulsed amperometric detector-high performance anion chromatography method for the determination of L-carnitine in health food was established.
  
  
  METHODS
  After samples were dissolved and dispersed with ether, L-carnitine in samples was extracted with 0. 1 mmol/L HC1. Using 200 mmol/L NaOH as the mobile phase, amino PAC PA10 Amino acid-separation column as separation column and pulse ampere detector with An as working electrode throughout the experiment.
  
  
  RESULTS
  The linear was good in the range of 0.08 - 1.00 mg/ml. The intra-day RSDs of peak area and retention time for L-carnitine standard solution were 3.97% and 0.62% and the inter-day RSDs were 5.66% and 0.69%, respectively. The average recoveries were 95.6% - 113.5% and the RSD of sample analysis was 7.34%. The results of the proposed method for the analysis of L-carnitine in health foods were satisfactory.
  
  
  CONCLUSIONS
  The proposed method is simple, accurate and reproducible. One analysis can be completed within 15 min. The method is adaptive for the analysis of L-carnitine in health food.
authors.affiliation: West China School of Public Health of Sichuan University, Chengdu 610041, China. zouxl_1113@163.com
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Zou
authors.forename: Yanyang
authors.initials: Y
authors.lastname: Zhou
authors.forename: Rong
authors.initials: R
authors.lastname: Qiao
authors.forename: Yuanqian
authors.initials: Y
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
mesh_terms: D002331:Carnitine / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods*; D019587:Dietary Supplements / Q000032:analysis*; D055664:Electrochemical Techniques / Q000379:methods; D058870:Food, Organic / Q000032:analysis*
chemical_list: D002331:Carnitine