title: MRM assay for quantitation of complement components in human blood plasma - a feasibility study on multiple sclerosis.
abstract: As a proof-of-principle study, a multiple reaction monitoring (MRM) assay was developed for quantitation of proteotypic peptides, representing seven plasma proteins associated with inflammation (complement components and C-reactive protein). The assay development and the sample analysis were performed on a linear ion trap mass spectrometer. We were able to quantify 5 of the 7 target proteins in depleted plasma digests with reasonable reproducibility over a 2 orders of magnitude linear range (RSD≤25%). The assay panel was utilized for the analysis of a small multiple sclerosis sample cohort with 10 diseased and 8 control patients.
authors.affiliation: Division of Clinical Protein Science & Imaging, Biomedical Center, Department of Measurement Technology and Industrial Electrical Engineering, Lund University, Lund, Sweden. melinda.rezeli@elmat.lth.se
authors.forename: Melinda
authors.initials: M
authors.lastname: Rezeli
authors.forename: Akos
authors.initials: A
authors.lastname: Végvári
authors.forename: Jan
authors.initials: J
authors.lastname: Ottervald
authors.forename: Tomas
authors.initials: T
authors.lastname: Olsson
authors.forename: Thomas
authors.initials: T
authors.lastname: Laurell
authors.forename: György
authors.initials: G
authors.lastname: Marko-Varga
mesh_terms: D002097:C-Reactive Protein / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D016022:Case-Control Studies; D003165:Complement System Proteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D005240:Feasibility Studies; D006801:Humans; D007249:Inflammation / Q000378:metabolism / Q000473:pathology; D013058:Mass Spectrometry / Q000379:methods; D009103:Multiple Sclerosis / Q000175:diagnosis* / Q000378:metabolism; D010455:Peptides / Q000032:analysis / Q000097:blood / Q000737:chemistry; D010949:Plasma / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D010455:Peptides; D003165:Complement System Proteins; D002097:C-Reactive Protein