title: Optimization of ultrasound-assisted extraction of anthocyanins from mulberry, using response surface methodology.
abstract: Mulberry is one of the most widely used traditional Chinese medicines. Anthocyanins are the main bioactive components of mulberry, and possess important biological activities, such as antimicrobial, anti-inflammatory and antioxidant activities. This study investigated the ultrasound-assisted extraction (UAE) of anthocyanins from mulberry by using response surface methodology (RSM). The extraction conditions associated with anthocyanin yield, including extraction solvent, liquid-to-solid rate, temperature and extraction time, are discussed. The optimal conditions obtained by RSM for UAE from mulberry include 63.8% methanol contains 1% (v/v) trifluoroacetic acid (TFA), 43.2 °C temperature, 23.8 (v/w) liquid-to-solid ratio, and 40 min time for the maximum yield (64.70 ± 0.45 mg/g). The results indicated that the UAE can be an effective method for the extraction of some active components from plant materials.
authors.affiliation: Department of Nutrition, School of Public Health, Sun Yat-sen University, Guangzhou 510080, China; E-Mails: zoutb@163.com (T.-B.Z.); wangmin1605@tom.com (M.W.); ganry_zsu@yahoo.cn (R.-Y.G.).
authors.forename: Tang-Bin
authors.initials: TB
authors.lastname: Zou
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.forename: Ren-You
authors.initials: RY
authors.lastname: Gan
authors.forename: Wen-Hua
authors.initials: WH
authors.lastname: Ling
mesh_terms: D000872:Anthocyanins / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D031625:Morus / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D014463:Ultrasonography / Q000379:methods*
chemical_list: D000872:Anthocyanins; D010936:Plant Extracts