title: Effect of β-phenylethyl isothiocyanate from cruciferous vegetables on growth inhibition and apoptosis of cervical cancer cells through the induction of death receptors 4 and 5.
abstract: Cruciferous vegetables have been shown to have the possibility to protect against multistep carcinogenesis. β-Phenylethyl isothiocyanate (PEITC) is one component of these vegetables demonstrated to help fight many types of cancer. The present study examined the apoptotic effects of PEITC and its molecular mechanism in human cervical cancer cell lines (HEp-2 and KB). PEITC induced apoptosis to inhibit cell proliferation. According to the protein chip assay, PEITC increased the expression of the death receptors (DR4 and DR5) and cleaved caspase-3 compared to the DMSO treatment group. PEITC also induced caspase-8 and truncated BID. PEITC down-regulated the phosphorylation of extracellular-related kinase (ERK)1/2, whereas neither phospho-c-Jun NH(2)-terminal kinases (JNK) nor phospho-p38 MAPK was changed. The role of ERK in PEITC-induced apoptosis was also investigated using MEK inhibitor (PD98059). PD98059 increased the expression of DR4 and DR5, activated caspase-3, and cleaved PARP. In addition, PEITC decreased the phosphorylation of MEK. Therefore, the apoptotic mechanism of PEITC in cervical cancer cells involves the induction of DR4 and DR5 through the inactivation of ERK and MEK.
authors.affiliation: Department of Oral Pathology, School of Dentistry, Institute of Oral Bioscience, Brain Korea 21, Chonbuk National University, Jeonju, Republic of Korea.
authors.forename: Le Diem
authors.initials: le D
authors.lastname: Huong
authors.forename: Jung-Hyung
authors.initials: JH
authors.lastname: Shim
authors.forename: Kyeong-Hee
authors.initials: KH
authors.lastname: Choi
authors.forename: Ji-Ae
authors.initials: JA
authors.lastname: Shin
authors.forename: Eun-Sun
authors.initials: ES
authors.lastname: Choi
authors.forename: Hyung-Seop
authors.initials: HS
authors.lastname: Kim
authors.forename: Sook-Jeong
authors.initials: SJ
authors.lastname: Lee
authors.forename: Sun-Ju
authors.initials: SJ
authors.lastname: Kim
authors.forename: Nam-Pyo
authors.initials: NP
authors.lastname: Cho
authors.forename: Sung-Dae
authors.initials: SD
authors.lastname: Cho
mesh_terms: D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D019607:Brassicaceae / Q000737:chemistry; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D005260:Female; D006801:Humans; D017879:Isothiocyanates / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology*; D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand / Q000235:genetics* / Q000378:metabolism; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology*; D014675:Vegetables / Q000737:chemistry
chemical_list: D016588:Anticarcinogenic Agents; D017879:Isothiocyanates; D010936:Plant Extracts; D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand; C058305:phenethyl isothiocyanate