title: Hydrogen peroxide modulates the dynamic microtubule cytoskeleton during the defence responses to Verticillium dahliae toxins in Arabidopsis.
abstract: The molecular mechanisms of signal transduction of plants in response to infection by Verticillium dahliae (VD) are not well understood. We previously showed that NO may act as an upstream signalling molecule to trigger the depolymerization of cortical microtubules in Arabidopsis. In the present study, we used the wild-type, and atrbohD and atrbohF mutants of Arabidopsis to explore the mechanisms of action of H(2)O(2) signals and the dynamic microtubule cytoskeleton in defence responses. We demonstrated that H(2)O(2) may also act as an upstream signalling molecule to regulate cortical microtubule depolymerization. The depolymerization of the cortical microtubules played a functional role in the signalling pathway to mediate the expression of defence genes. The results indicate that H(2)O(2) modulates the dynamic microtubule cytoskeleton to trigger the expression of defence genes against V. dahliae toxins (VD-toxins) in Arabidopsis.
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China.
authors.forename: Lin-Lin
authors.initials: LL
authors.lastname: Yao
authors.forename: Qun
authors.initials: Q
authors.lastname: Zhou
authors.forename: Bao-Lei
authors.initials: BL
authors.lastname: Pei
authors.forename: Ying-Zhang
authors.initials: YZ
authors.lastname: Li
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000502:physiology* / Q000648:ultrastructure; D029681:Arabidopsis Proteins / Q000235:genetics; D003599:Cytoskeleton / Q000187:drug effects* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D006861:Hydrogen Peroxide / Q000378:metabolism / Q000494:pharmacology*; D008870:Microtubules / Q000187:drug effects / Q000378:metabolism; D009183:Mycotoxins / Q000302:isolation & purification / Q000494:pharmacology*; D010935:Plant Diseases / Q000382:microbiology; D057865:Plant Immunity; D018515:Plant Leaves / Q000235:genetics / Q000502:physiology / Q000648:ultrastructure; D030821:Plants, Genetically Modified / Q000235:genetics / Q000502:physiology / Q000648:ultrastructure; D018749:RNA, Plant / Q000235:genetics; D036226:Seedlings / Q000235:genetics / Q000502:physiology / Q000648:ultrastructure; D017384:Sequence Deletion; D015398:Signal Transduction / Q000187:drug effects; D020172:Verticillium / Q000737:chemistry*
chemical_list: D029681:Arabidopsis Proteins; D009183:Mycotoxins; D018749:RNA, Plant; D006861:Hydrogen Peroxide