title: 8,12;8,20-diepoxy-8,14-secopregnane glycosides from roots of Asclepias tuberosa and their effect on proliferation of human skin fibroblasts.
abstract: A pregnane glycoside fraction from the roots of Asclepias tuberosa L. caused normal human skin fibroblasts to proliferate. This fraction contained 21 pregnane glycosides whose structures were established using NMR spectroscopic analysis and chemical evidence. The aglycones of most of these compounds were identified as 8,12;8,20-diepoxy-8,14-secopregnanes, such as tuberogenin or 5,6-didehydrotuberogenin, the same aglycones as constituents of the aerial parts of this plant. Some of these compounds also caused proliferation of skin fibroblasts.
authors.affiliation: Institute for Environmental Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, Japan. warashin@u-shizuoka-ken.ac.jp
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Warashina
authors.forename: Kaoru
authors.initials: K
authors.lastname: Umehara
authors.forename: Toshio
authors.initials: T
authors.lastname: Miyase
authors.forename: Tadataka
authors.initials: T
authors.lastname: Noro
mesh_terms: D031108:Asclepias / Q000737:chemistry*; D049109:Cell Proliferation / Q000187:drug effects; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D011278:Pregnanes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D012632:Secosteroids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D013256:Steroids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D006027:Glycosides; D010936:Plant Extracts; D011278:Pregnanes; D012503:Saponins; D012632:Secosteroids; D013256:Steroids; C436828:tuberoside