title: In vitro adherence of Candida albicans isolated from patients with chronic periodontitis.
abstract: Adherence is considered an extremely important virulence factor in yeast.
  
  
  OBJECTIVE
  The aim of this study was to analyze the adherence to epithelial cells of C. albicans isolated from patients with chronic periodontitis in comparison to healthy patients.
  
  
  METHODS
  Candida albicans cells isolated from individuals with chronic periodontitis (n=25) and healthy controls (n=25) were included in this study. Suspensions of C. albicans (10(6) cells/mL) and epithelial cells (10(5) cells/mL) were mixed and incubated at 37ºC for 1 h. The number of yeasts adhered to 25 epithelial cells was counted.
  
  
  RESULTS
  The number of C. albicans cells adhered to epithelial cells was statistically higher in the chronic periodontitis group than in the control group (Student's t-test, p=0.000).
  
  
  CONCLUSIONS
  The results of the present study suggest a higher Candida adherence of samples isolated from patients with chronic periodontitis.
authors.affiliation: Department of Biosciences and Oral Diagnosis, São José dos Campos Dental School, State University of São Paulo, São José dos Campos, Brazil.
authors.forename: Adriana Gadotti
authors.initials: AG
authors.lastname: Machado
authors.forename: Edson Yukio
authors.initials: EY
authors.lastname: Komiyama
authors.forename: Silvana Soléo Ferreira Dos
authors.initials: SS
authors.lastname: Santos
authors.forename: Antonio Olavo Cardoso
authors.initials: AO
authors.lastname: Jorge
authors.forename: Fernanda Lourenção
authors.initials: FL
authors.lastname: Brighenti
authors.forename: Cristiane Yumi
authors.initials: CY
authors.lastname: Koga-Ito
mesh_terms: D000328:Adult; D002176:Candida albicans / Q000302:isolation & purification / Q000502:physiology*; D016022:Case-Control Studies; D002448:Cell Adhesion; D055113:Chronic Periodontitis / Q000382:microbiology*; D015169:Colony Count, Microbial; D004847:Epithelial Cells / Q000382:microbiology*; D006801:Humans; D008875:Middle Aged; D009061:Mouth Mucosa / Q000382:microbiology; D037521:Virulence Factors
chemical_list: D037521:Virulence Factors