title: Use of GFP to trace the colonization of Lactococcus lactis WH-C1 in the gastrointestinal tract of mice.
abstract: A new expression vector for Lactococcus was constructed using nisI as a selection marker and GFP as a reporter protein to explore the colonization characteristics in vivo of Lactococcus lactis WH-C1. By high expression of GFP, it was shown WH-C1 could pass through the stomach and survive in the gastrointestinal tract.
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Ministry of Education, Tianjin 300457, China. ypwang40@yahoo.com
authors.forename: Yanping
authors.initials: Y
authors.lastname: Wang
authors.forename: Jingrui
authors.initials: J
authors.lastname: Wang
authors.forename: Weili
authors.initials: W
authors.lastname: Dai
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics*; D041981:Gastrointestinal Tract / Q000382:microbiology*; D017930:Genes, Reporter; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics* / Q000378:metabolism; D013294:Lactococcus lactis / Q000254:growth & development* / Q000378:metabolism; D008074:Lipoproteins / Q000096:biosynthesis* / Q000235:genetics*; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics*; D051379:Mice
chemical_list: D001426:Bacterial Proteins; D008074:Lipoproteins; D008565:Membrane Proteins; C094909:NisI protein, Lactococcus lactis; D049452:Green Fluorescent Proteins