title: Incomplete folding upon binding mediates Cdk4/cyclin D complex activation by tyrosine phosphorylation of inhibitor p27 protein.
abstract: p27(Kip1) (p27), an intrinsically disordered protein, regulates the various Cdk/cyclin complexes that control cell cycle progression. The kinase inhibitory domain of p27 contains a cyclin-binding subdomain (D1), a Cdk-binding subdomain (D2), and a linker helix subdomain that connects D1 and D2. Here, we report that, despite extensive sequence conservation between Cdk4/cyclin D1 (hereafter Cdk4/cyclin D) and Cdk2/cyclin A, the thermodynamic details describing how the individual p27 subdomains contribute to equally high affinity binding to these two Cdk/cyclin complexes are strikingly different. Differences in enthalpy/entropy compensation revealed that the D2 subdomain of p27 folds incompletely when binding Cdk4/cyclin D versus Cdk2/cyclin A. Incomplete binding-induced folding exposes tyrosine 88 of p27 for phosphorylation by the nonreceptor tyrosine kinase Abl. Importantly, tyrosine phosphorylation (of p27) relieves Cdk inhibition by p27, enabling cell cycle entry. Furthermore, the interaction between a conserved hydrophobic patch on cyclin D and subdomain D1 is much weaker than that with cyclin A; consequently, a construct containing subdomains D1 and LH (p27-D1LH) does not inhibit substrate binding to Cdk4/cyclin D as it does to Cdk2/cyclin A. Our results provide a mechanism by which Cdk4 (within the p27/Cdk4/cyclin D complex) is poised to be activated by extrinsic mitogenic signals that impinge upon p27 at the earliest stage of cell division. More broadly, our results further illustrate the regulatory versatility of intrinsically disordered proteins.
authors.affiliation: Department of Structural Biology, St. Jude Children's Research Hospital, Memphis, Tennessee 38105, USA.
authors.forename: Li
authors.initials: L
authors.lastname: Ou
authors.forename: Antonio M
authors.initials: AM
authors.lastname: Ferreira
authors.forename: Steve
authors.initials: S
authors.lastname: Otieno
authors.forename: Limin
authors.initials: L
authors.lastname: Xiao
authors.forename: Donald
authors.initials: D
authors.lastname: Bashford
authors.forename: Richard W
authors.initials: RW
authors.lastname: Kriwacki
mesh_terms: D056741:Cyclin D / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D051358:Cyclin-Dependent Kinase 4 / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D019277:Entropy; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D046912:Multiprotein Complexes / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D010766:Phosphorylation / Q000502:physiology; D011485:Protein Binding; D017510:Protein Folding; D017434:Protein Structure, Tertiary; D016315:Proto-Oncogene Proteins c-abl / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: C436291:CDKN1B protein, human; D056741:Cyclin D; D046912:Multiprotein Complexes; D050760:Cyclin-Dependent Kinase Inhibitor p27; D016315:Proto-Oncogene Proteins c-abl; C495900:CDK4 protein, human; D051358:Cyclin-Dependent Kinase 4