title: Sulfonated cyclofructan 6 based stationary phase for hydrophilic interaction chromatography.
abstract: A stationary phase composed of silica-bonded sulfonated cyclofructan 6 (SCF6) was synthesized and evaluated for hydrophilic interaction chromatography (HILIC). The separation of a large variety of polar compounds was evaluated on different versions of the stationary phase and compared with the same separations obtained with commercially available HILIC columns. The new columns successfully separate polar and hydrophilic compounds including β blockers, xanthines, salicylic acid related compounds, nucleic acid bases, nucleosides, maltooligosaccharides, water soluble vitamins and amino acids. The separation conditions were optimized by changing the composition and the pH of the mobile phase. The dependence of analyte retention on temperature was studied using van't Hoff plots. The newly synthesized stationary phase showed broad applicability for HILIC mode separations.
authors.affiliation: Department of Chemistry and Biochemistry, University of Texas at Arlington, Arlington, TX 76019, USA.
authors.forename: Nilusha L T
authors.initials: NL
authors.lastname: Padivitage
authors.forename: Daniel W
authors.initials: DW
authors.lastname: Armstrong
mesh_terms: D000327:Adsorption; D000596:Amino Acids / Q000032:analysis / Q000302:isolation & purification; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D005630:Fructans / Q000138:chemical synthesis / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D009696:Nucleic Acids / Q000032:analysis / Q000302:isolation & purification; D009705:Nucleosides / Q000032:analysis / Q000302:isolation & purification; D004364:Pharmaceutical Preparations / Q000032:analysis / Q000302:isolation & purification
chemical_list: D000596:Amino Acids; D005630:Fructans; D009696:Nucleic Acids; D009705:Nucleosides; D004364:Pharmaceutical Preparations; C557358:cyclofructan 6