title: Chemical analysis and study of immunoenhancing and antioxidant property of a glucan isolated from an alkaline extract of a somatic hybrid mushroom of Pleurotus florida and Calocybe indica variety APK2.
abstract: A water-soluble glucan isolated from an alkaline extract of fruit bodies of a somatic hybrid mushroom PCH9FB of Pleurotus florida and Calocybe indica var. APK2 strains showed antioxidant properties with immune activation of macrophage, splenocyte, and thymocyte. On the basis of acid hydrolysis, methylation, periodate oxidation and NMR studies ((1)H, (13)C, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HMQC, and HMBC) the structure of the repeating unit of the glucan was established as: [structure: see text].
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore, West Bengal, India.
authors.forename: Kousik
authors.initials: K
authors.lastname: Maity
authors.forename: Eshita
authors.initials: E
authors.lastname: Kar Mandal
authors.forename: Saikat
authors.initials: S
authors.lastname: Maity
authors.forename: Sanjoy K
authors.initials: SK
authors.lastname: Gantait
authors.forename: Debsankar
authors.initials: D
authors.lastname: Das
authors.forename: Swatilekha
authors.initials: S
authors.lastname: Maiti
authors.forename: Tapas K
authors.initials: TK
authors.lastname: Maiti
authors.forename: Samir R
authors.initials: SR
authors.lastname: Sikdar
authors.forename: Syed S
authors.initials: SS
authors.lastname: Islam
mesh_terms: D000468:Alkalies / Q000737:chemistry*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D005936:Glucans / Q000138:chemical synthesis / Q000276:immunology* / Q000302:isolation & purification* / Q000494:pharmacology; D006824:Hybridization, Genetic; D008262:Macrophage Activation / Q000187:drug effects; D017737:Macrophages, Peritoneal / Q000166:cytology / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D009569:Nitric Oxide / Q000494:pharmacology; D020076:Pleurotus / Q000737:chemistry*; D013154:Spleen / Q000166:cytology; D060168:Thymocytes / Q000166:cytology / Q000187:drug effects
chemical_list: D000468:Alkalies; D000975:Antioxidants; D005936:Glucans; D009569:Nitric Oxide