title: Oxidases and oxygenases in regulation of neutrophil redox pathways in Behçet's disease patients.
abstract: This study aimed to determine plasma and neutrophil oxidase activities that may contribute to vascular inflammation in Behçet's disease (BD) patients. Cyclooxygenase (COX), NADPH oxidase and myeloperoxidase (MPO) activity was determined in neutrophils isolated from BD patients and healthy controls. Functional assay of NADPH oxidase was significantly increased in BD patients, both at basal conditions and in response to fMLP stimulation. There was a significant increase in plasma MPO activity in the disease group as compared to controls. Total COX activity was significantly increased in BD neutrophils. The increase in total COX activity was accompanied with enhanced activity of COX-2, differentiated by using the COX-1 isoform-specific inhibitor SC-560. Neutrophil nitrate/nitrite levels showed no significant difference in BD; however, plasma nitrate/nitrite contents in BD patients were significantly greater compared to controls. In conclusion, increased plasma MPO, neutrophil NADPH and COX activities may contribute to intravascular inflammation documented in BD patients.
authors.affiliation: Department of Physical Medicine-Rheumatology, Faculty of Medicine, Akdeniz University, Antalya, Turkey.
authors.forename: Meltem A
authors.initials: MA
authors.lastname: Melikoglu
authors.forename: Cahit
authors.initials: C
authors.lastname: Kacar
authors.forename: Aysegul
authors.initials: A
authors.lastname: Cort
authors.forename: Gultekin
authors.initials: G
authors.lastname: Yucel
authors.forename: Mutay
authors.initials: M
authors.lastname: Aslan
mesh_terms: D000328:Adult; D001528:Behcet Syndrome / Q000201:enzymology* / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D019255:NADPH Oxidases / Q000097:blood / Q000378:metabolism*; D009504:Neutrophils / Q000201:enzymology / Q000378:metabolism*; D009566:Nitrates / Q000097:blood; D009569:Nitric Oxide / Q000096:biosynthesis; D009573:Nitrites / Q000097:blood; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000097:blood / Q000378:metabolism*; D011451:Prostaglandin-Endoperoxide Synthases / Q000097:blood / Q000378:metabolism*; D055815:Young Adult
chemical_list: D009566:Nitrates; D009573:Nitrites; D009569:Nitric Oxide; D009195:Peroxidase; D011451:Prostaglandin-Endoperoxide Synthases; D019255:NADPH Oxidases