title: Allosteric modulation of retinal GABA receptors by ascorbic acid.
abstract: Ionotropic GABA receptors (GABA(A) and GABA(C)) belong to the Cys-loop receptor family of ligand-gated ion channels. GABA(C) receptors are highly expressed in the retina, mainly localized at the axon terminals of bipolar cells. Ascorbic acid, an endogenous redox agent, modulates the function of diverse proteins, and basal levels of ascorbic acid in the retina are very high. However, the effect of ascorbic acid on retinal GABA receptors has not been studied. Here we show that the function of GABA(C) and GABA(A) receptors is regulated by ascorbic acid. Patch-clamp recordings from bipolar cell terminals in goldfish retinal slices revealed that GABA(C) receptor-mediated currents activated by tonic background levels of extracellular GABA, and GABA(C) currents elicited by local GABA puffs, are both significantly enhanced by ascorbic acid. In addition, a significant rundown of GABA puff-evoked currents was observed in the absence of ascorbic acid. GABA-evoked Cl(-) currents mediated by homomeric ρ(1) GABA(C) receptors expressed in Xenopus laevis oocytes were also potentiated by ascorbic acid in a concentration-dependent, stereo-specific, reversible, and voltage-independent manner. Studies involving the chemical modification of sulfhydryl groups showed that the two Cys-loop cysteines and histidine 141, all located in the ρ(1) subunit extracellular domain, each play a key role in the modulation of GABA(C) receptors by ascorbic acid. Additionally, we show that retinal GABA(A) IPSCs and heterologously expressed GABA(A) receptor currents are similarly augmented by ascorbic acid. Our results suggest that ascorbic acid may act as an endogenous agent capable of potentiating GABAergic neurotransmission in the CNS.
authors.affiliation: Laboratorio de Neurobiología Celular y Molecular, Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad de Buenos Aires, CP 1428, Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Cecilia I
authors.initials: CI
authors.lastname: Calero
authors.forename: Evan
authors.initials: E
authors.lastname: Vickers
authors.forename: Gustavo
authors.initials: G
authors.lastname: Moraga Cid
authors.forename: Luis G
authors.initials: LG
authors.lastname: Aguayo
authors.forename: Henrique
authors.initials: H
authors.lastname: von Gersdorff
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Calvo
mesh_terms: D000494:Allosteric Regulation; D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism / Q000494:pharmacology*; D002478:Cells, Cultured; D055724:Electrophysiological Phenomena; D005260:Female; D006054:Goldfish; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D018079:Receptors, GABA / Q000378:metabolism*; D012160:Retina / Q000187:drug effects* / Q000378:metabolism; D051245:Retinal Bipolar Cells / Q000187:drug effects* / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000378:metabolism / Q000494:pharmacology
chemical_list: D018079:Receptors, GABA; D005680:gamma-Aminobutyric Acid; D001205:Ascorbic Acid