title: Caloric restriction improves coagulation and inflammation profile in obese mice.
abstract: To evaluate associations between adiposity and coagulation or inflammation profile, obese wild-type C57Bl/6 mice were subjected to drastic caloric restriction by switching from a high fat diet to restricted normal chow. After 6 weeks, total body weights as well as subcutaneous and gonadal adipose tissue mass were markedly reduced, associated with adipocyte hypotrophy (all p<0.001). Weight reduction was associated with markedly reduced plasma levels of plasminogen activator inhibitor-1, Factor VII and Factor VIII. Reduced oxidative stress and inflammation following weight reduction is supported by significantly lower expression in adipose tissues of pro-inflammatory interleukin-6, higher expression of anti-oxidant catalase, superoxide dismutase 1 and glutathione peroxidase 1, and lower plasma levels of C-reactive protein. Furthermore, reduced levels of leptin and enhanced levels of adiponectin were observed, whereas cholesterol and triglyceride levels were reduced. The content of structurally intact collagen fibers was significantly higher in subcutaeneous and gonadal adipose tissues after caloric restriction. Thus, caloric restriction and drastic weight loss in obese mice is associated with improved plasma coagulation profile and with reduced oxidative stress and inflammation in the adipose tissues.
authors.affiliation: Center for Molecular and Vascular Biology, KU Leuven, Leuven, Belgium. roger.lijnen@med.kuleuven.be
authors.forename: H Roger
authors.initials: HR
authors.lastname: Lijnen
authors.forename: Matthias
authors.initials: M
authors.lastname: Van Hul
authors.forename: Bianca
authors.initials: B
authors.lastname: Hemmeryckx
mesh_terms: D000273:Adipose Tissue / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D050154:Adiposity; D000818:Animals; D015415:Biomarkers / Q000097:blood; D001777:Blood Coagulation; D031204:Caloric Restriction; D004195:Disease Models, Animal; D024022:Fibrillar Collagens / Q000378:metabolism; D005786:Gene Expression Regulation; D006984:Hypertrophy; D007249:Inflammation / Q000097:blood / Q000235:genetics / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D018836:Inflammation Mediators / Q000097:blood*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000097:blood / Q000150:complications / Q000178:diet therapy* / Q000235:genetics / Q000276:immunology / Q000473:pathology; D018384:Oxidative Stress; D012333:RNA, Messenger / Q000378:metabolism; D013997:Time Factors; D015431:Weight Loss
chemical_list: D015415:Biomarkers; D024022:Fibrillar Collagens; D018836:Inflammation Mediators; D012333:RNA, Messenger