title: Vitamin E and polyunsaturated fatty acids in bovine muscle and the oxidative stability of beef from cattle receiving grass or concentrate-based rations.
abstract: The present study was designed to assess the balance between antioxidant and prooxidant components and the oxidative stability of beef from cattle fed exclusively grazed pasture (PAS) or a barley-based concentrate offered indoors (CONC) for 11 mo, or fed grass silage indoors for a 5-mo winter period, followed for the remaining 6-mo summer period by grazed pasture (SiP) or by grazed pasture plus concentrate at 50% of the dietary DM (SiPC). Muscle prooxidant and antioxidant components were determined by measuring fatty acids and α-tocopherol concentration of LM, respectively. Lipid oxidation and color stability were monitored in ground LM, packaged in a high-oxygen modified atmosphere, over 11 d of refrigerated storage. Vitamin E concentration decreased (P < 0.0005) with an increasing proportion of concentrate in the diet (2.59, 2.45, 1.76, and 1.15 μg/g for PAS, SiP, SiPC, and CONC, respectively). A greater proportion of PUFA was found in LM from cattle in the PAS, SiP, and SiPC groups compared with animals in the CONC group (9.62, 11.04, 8.96, and 6.94%, respectively; P < 0.0005). A greater concentration of highly peroxidizable PUFA was found in LM from heifers in the PAS, SiP, and SiPC groups compared with those in the CONC group (0.84, 0.85, 0.87, and 0.65 mg/g of muscle, respectively; P = 0.02). Dietary treatment affected lipid oxidation (P < 0.0005), with greater 2-thiobarbituric acid reactive substance values in beef from heifers in the SiPC group than in beef from those in the PAS, SiP, and CONC groups. Dietary treatment affected myoglobin oxidation (P = 0.002) during storage, with greater metmyoglobin accumulation in beef from animals receiving concentrate (CONC and SiPC treatments) than in beef from cattle in the PAS and SiP groups. Consequently, feeding concentrate impaired meat color stability over the storage duration, with greater H* (hue angle) values (P < 0.0005) in meat from heifers in the SiPC and CONC groups compared with meat from those in the PAS and SiP groups. The results of the present study confirm a positive effect of grass-based feeding systems on meat color stability compared with concentrate-based dietary strategies. It appears that vitamin E in muscle alone does not explain the resistance of meat to oxidative deterioration because a clear interaction with highly peroxidizable PUFA exists.
authors.affiliation: Dipartimento di Scienze delle Produzioni Agrarie e Alimentari (DISPA), University of Catania, Via Valdisavoia 5, 95123, Catania, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Luciano
authors.forename: A P
authors.initials: AP
authors.lastname: Moloney
authors.forename: A
authors.initials: A
authors.lastname: Priolo
authors.forename: F T
authors.initials: FT
authors.lastname: Röhrle
authors.forename: V
authors.initials: V
authors.lastname: Vasta
authors.forename: L
authors.initials: L
authors.lastname: Biondi
authors.forename: P
authors.initials: P
authors.lastname: López-Andrés
authors.forename: S
authors.initials: S
authors.lastname: Grasso
authors.forename: F J
authors.initials: FJ
authors.lastname: Monahan
mesh_terms: D000821:Animal Feed; D000822:Animal Husbandry / Q000379:methods*; D000818:Animals; D002417:Cattle / Q000378:metabolism*; D004032:Diet / Q000662:veterinary*; D005231:Fatty Acids, Unsaturated / Q000097:blood; D005260:Female; D008460:Meat / Q000592:standards*; D008786:Metmyoglobin / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism*; D006109:Poaceae; D011897:Random Allocation; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D024502:alpha-Tocopherol / Q000032:analysis*
chemical_list: D005231:Fatty Acids, Unsaturated; D017392:Thiobarbituric Acid Reactive Substances; D008786:Metmyoglobin; D024502:alpha-Tocopherol