title: Neuroprotective effect of vitamin C against PTZ induced apoptotic neurodegeneration in adult rat brain.
abstract: The present study was designed to observe the effect of PTZ on expression of caspsae-3, and to evaluate the neuroprotective role of vitamin C (vit-C) against PTZ-induced apoptotic neurodegeneration in adult rat brain. We observed that administration of a single conclusive dose of pentylenetetrazol (PTZ 50mg/kg) in adults rats induced epileptic seizure and increased activation of caspase-3 and caused neuronal death. Further, rats were injected with vit-C (250 mg/kg) 30 min before PTZ injection. The protective effect of vit-C against PTZ-induced apoptotic neurodegeneration in adult rat brain was observed using Western blot analysis and Nissl staining. The results showed that conclusive dose of PTZ-induced seizure, increased expression of caspase-3 and neuronal apoptosis in adult rat brain. Whereas, the pretreatment of vit-C along with PTZ showed significantly decreased expression of caspase-3 as compare to control group. Finally, our results indicated that vit-C can prevent some of the deleterious effect of seizure and neuronal degeneration induced by PTZ in adult rat brain.
authors.affiliation: Department of Biology, College of Natural Sciences (RINS) and Applied Life Science (BK 21), Gyeongsang National University, Jinju, Republic of Korea.
authors.forename: Muhammad Imran
authors.initials: MI
authors.lastname: Naseer
authors.forename: Ikram
authors.initials: I
authors.lastname: Ullah
authors.forename: Najeeb
authors.initials: N
authors.lastname: Ullah
authors.forename: Hae Young
authors.initials: HY
authors.lastname: Lee
authors.forename: Eun Woo
authors.initials: EW
authors.lastname: Cheon
authors.forename: Jongil
authors.initials: J
authors.lastname: Chung
authors.forename: Myeong Ok
authors.initials: MO
authors.lastname: Kim
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001205:Ascorbic Acid / Q000494:pharmacology / Q000627:therapeutic use*; D001921:Brain / Q000187:drug effects / Q000201:enzymology / Q000473:pathology*; D053148:Caspase 3 / Q000378:metabolism; D004195:Disease Models, Animal; D004827:Epilepsy / Q000139:chemically induced / Q000201:enzymology / Q000473:pathology*; D008297:Male; D009410:Nerve Degeneration / Q000139:chemically induced / Q000201:enzymology / Q000517:prevention & control*; D018696:Neuroprotective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D010433:Pentylenetetrazole / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D018696:Neuroprotective Agents; D053148:Caspase 3; D001205:Ascorbic Acid; D010433:Pentylenetetrazole