title: Profiles of MMP-2 expression in Jurkat, Molt-4 and U937 cells.
abstract: BACKGROUND
  Leukemia is a malignant proliferative disorder of the hematopoietic cells. The important role of angiogenesis in leukemia has been reported by several studies. Matrix metalloproteinases (MMPs) are a large group of endopeptidases which degredate the extracellular matrix and play an important role in angiogenesis.
  
  
  OBJECTIVE
  The present study was conducted to evaluate the patterns of MMP-2 activity in three leukemic cell lines.
  
  
  METHODS
  Human leukemic monocyte (U937) and T cells (Molt-4 and Jurkat) were cultured in complete RPMI-1640 medium. The cells were then seeded at a density of 106 cells/ml and were incubated with different concentrations of phorbol myristate acetate (PMA) (1-25 ng/ml) or phytoheamagglutinin (PHA) (2-10 µg/ml) for 24 hours. The MMP-2 activity in cell-conditioned media was then evaluated by gelatin zymography. Statistical comparisons between groups were made by analysis of variance (ANOVA).
  
  
  RESULTS
  PHA/PMA significantly and dose-dependently increased MMP-2 activity in U937 cells after 24 hours of incubation compared with untreated control cells. Moreover, PHA/PMA significantly induced MMP-2 activity in Molt-4 and Jurkat cells after 24 hours of incubation in a dose-dependent manner compared with untreated control cells.
  
  
  CONCLUSIONS
  We conclude that human leukemic Jurkat, U937 and Molt-4 cells could potentially display MMP-2 activity with different degrees. Thus, these cell lines could provide an appropriate system to study the mechanisms regulating MMPs production in leukemia patients.
authors.affiliation: Department of Immunology, Shahed University, Tehran, Iran. resoome@yahoo.com
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Hajighasemi
mesh_terms: D018929:Cell Culture Techniques; D020869:Gene Expression Profiling; D006801:Humans; D056747:Immunomodulation; D019169:Jurkat Cells / Q000276:immunology*; D007938:Leukemia / Q000235:genetics / Q000276:immunology / Q000378:metabolism* / Q000473:pathology*; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D010835:Phytohemagglutinins / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000378:metabolism; D020298:U937 Cells / Q000276:immunology*
chemical_list: D010835:Phytohemagglutinins; D020778:Matrix Metalloproteinase 2; D013755:Tetradecanoylphorbol Acetate