title: Solid-phase synthesis of BODIPY dyes and development of an immunoglobulin fluorescent sensor.
abstract: The diversification of the BODIPY scaffold has been hindered by its controversial adaptability to solid-phase chemistry. Herein we report the first solid-phase synthesis of a BODIPY library in high purities. We screened the library against a set of proteins, identified an immunoglobulin fluorescent sensor (Ig Orange) and confirmed its binding by SPR experiments.
authors.affiliation: Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, Agency for Science, Technology and Research, Singapore.
authors.forename: Marc
authors.initials: M
authors.lastname: Vendrell
authors.forename: Gaddamanugu Gopi
authors.initials: GG
authors.lastname: Krishna
authors.forename: Krishna Kanta
authors.initials: KK
authors.lastname: Ghosh
authors.forename: Duanting
authors.initials: D
authors.lastname: Zhai
authors.forename: Jun-Seok
authors.initials: JS
authors.lastname: Lee
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhu
authors.forename: Yin Hoe
authors.initials: YH
authors.lastname: Yau
authors.forename: Susana Geifman
authors.initials: SG
authors.lastname: Shochat
authors.forename: Hyori
authors.initials: H
authors.lastname: Kim
authors.forename: Junho
authors.initials: J
authors.lastname: Chung
authors.forename: Young-Tae
authors.initials: YT
authors.lastname: Chang
mesh_terms: D001896:Boron Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D004578:Electron Spin Resonance Spectroscopy; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D011485:Protein Binding; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D005456:Fluorescent Dyes; D007074:Immunoglobulin G