title: A near-IR reversible fluorescent probe modulated by selenium for monitoring peroxynitrite and imaging in living cells.
abstract: We have developed a near-IR reversible fluorescent probe containing an organoselenium functional group that can be used for the highly sensitive and selective monitoring of peroxynitrite oxidation and reduction events under physiological conditions. The probe effectively avoids the influence of autofluorescence in biological systems and gave positive results when tested in both aqueous solution and living cells. Real-time images of cellular peroxynitrite were successfully acquired.
authors.affiliation: State Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics (DICP), Chinese Academy of Sciences (CAS), 457 Zhongshan Road, Dalian 116023, P. R. China.
authors.forename: Fabiao
authors.initials: F
authors.lastname: Yu
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.forename: Guangyue
authors.initials: G
authors.lastname: Li
authors.forename: Guangjiu
authors.initials: G
authors.lastname: Zhao
authors.forename: Tianshu
authors.initials: T
authors.lastname: Chu
authors.forename: Keli
authors.initials: K
authors.lastname: Han
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002460:Cell Line; D005456:Fluorescent Dyes / Q000737:chemistry*; D008264:Macrophages / Q000166:cytology; D051379:Mice; D018613:Microscopy, Confocal; D016566:Organoselenium Compounds / Q000737:chemistry*; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D030421:Peroxynitrous Acid / Q000032:analysis*; D012680:Sensitivity and Specificity
chemical_list: D005456:Fluorescent Dyes; D016566:Organoselenium Compounds; D030421:Peroxynitrous Acid