title: Migration responses of outer and inner meniscus cells to applied direct current electric fields.
abstract: Injuries to the inner regions of the knee meniscus do not heal and can result in degenerative changes to the articular surface, ultimately leading to osteoarthritis. A possible stimulus to enhance meniscus healing is to use electric fields that induce galvanotaxis. In this study, a novel characterization of the effects of direct current electric fields on migration characteristics of meniscus cells was performed. Primary and passaged inner and outer meniscus cells were exposed to varying electric field strengths from 0 to 6 V/cm. Cell migration was tracked using time lapse digital photography, and cell displacement and cathodal direct velocity were quantified. Cytoskeletal staining was performed to examine actin distribution and nuclear content. Cell adhesion strength was quantified as a function of wall shear stress. Meniscus cells exhibited cathodal migration and cell elongation perpendicular to the applied electric field accompanied by actin reorganization. Outer meniscus cells migrated quicker and exhibited lower adhesion strengths when compared to inner meniscus cells. Passaged cells exhibited higher migration characteristics when compared to primary cells. Overall, this study demonstrated that electric fields can significantly enhance and direct meniscus cell migration and suggests the potential for their incorporation in strategies of meniscus repair and tissue engineering.
authors.affiliation: Department of Biomedical Engineering, Columbia University, New York, NY 10027, USA.
authors.forename: Najmuddin J
authors.initials: NJ
authors.lastname: Gunja
authors.forename: Divya
authors.initials: D
authors.lastname: Dujari
authors.forename: Andrew
authors.initials: A
authors.lastname: Chen
authors.forename: Alba
authors.initials: A
authors.lastname: Luengo
authors.forename: Jason V
authors.initials: JV
authors.lastname: Fong
authors.forename: Clark T
authors.initials: CT
authors.lastname: Hung
mesh_terms: D000818:Animals; D002417:Cattle; D002448:Cell Adhesion / Q000502:physiology / Q000528:radiation effects; D002465:Cell Movement / Q000502:physiology / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D004558:Electric Stimulation / Q000379:methods*; D004599:Electric Stimulation Therapy / Q000379:methods; D004574:Electromagnetic Fields; D008592:Menisci, Tibial / Q000166:cytology* / Q000502:physiology / Q000528:radiation effects*; D010003:Osteoarthritis / Q000503:physiopathology / Q000532:radiotherapy; D023822:Tissue Engineering / Q000379:methods; D014945:Wound Healing / Q000502:physiology / Q000528:radiation effects*