title: MAGI1 inhibits cancer cell migration and invasion of hepatocellular carcinoma via regulating PTEN.
abstract: OBJECTIVE
  To explore the biological function and molecular mechanism of membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1) in hepatocellular carcinoma.
  
  
  METHODS
  HepG2(MAGI1) stable cell line was constructed by transfecting HepG2 cells with pcDNA3.1-MAGI1 plasmid. Wound healing and invasion assay were performed to compare the migration and invasion ability of HepG2(MAGI1) and HepG2 cells. Furthermore, the expression of MAGI1 and phosphatase and tensin homolog deleted on chromosome ten (PTEN) was also examined by Western blot and the relationship was analyzed.
  
  
  RESULTS
  The wound healing assay showed that the closure of HepG2(MAGI1) cells was significantly slower than that of HepG2 cells [(90 ± 10)% vs. (50 ± 15)%, P<0.05], and the invasion assay showed that the number of HepG2(MAGI1) cells that passed through the matrigel was fewer than HepG2 cells (68 ± 18 vs. 150 ± 30, P<0.05). The protein expression level of PTEN was significantly elevated in HepG2(MAGI1) cells compared with HepG2 cells (1.40 ± 0.32 vs. 0.28 ± 0.15, P<0.05). MAGI1 and PTEN protein expression levels were positively correlated (r=0.913, P<0.01).
  
  
  CONCLUSIONS
  MAGI1 may inhibit the cancer cell migration and invasion of hepatocellular carcinoma via regulating PTEN.
authors.affiliation: Department of Surgery, Xiangya Hospital, Central South University, Changsha 410008, China. zyxh888@163.com
authors.forename: Gewen
authors.initials: G
authors.lastname: Zhang
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Wang
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D015815:Cell Adhesion Molecules; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics / Q000378:metabolism*; D002465:Cell Movement; D015972:Gene Expression Regulation, Neoplastic; D051528:Guanylate Kinases; D056945:Hep G2 Cells; D006801:Humans; D009361:Neoplasm Invasiveness; D051059:PTEN Phosphohydrolase / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D015815:Cell Adhesion Molecules; D015816:Cell Adhesion Molecules, Neuronal; D051528:Guanylate Kinases; C113338:MAGI1 protein, human; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human