title: Spatial and temporal bias in the mitotic origins of somatostatin- and parvalbumin-expressing interneuron subgroups and the chandelier subtype in the medial ganglionic eminence.
abstract: GABAergic interneurons modulate cortical activity through the actions of distinct subgroups. Recent studies using interneuron transplants have shown tremendous promise as cell-based therapies for seizure disorders, Parkinson's disease, and in the study of neocortical plasticity. Previous reports identified a spatial bias for the origins of parvalbumin (PV)- and somatostatin (SST)-expressing interneuron subgroups within the medial ganglionic eminence (MGE). In the current study, the mitotic origins of these interneurons are examined by harvesting MGE cells at 2 time points and evaluating their neurochemical profiles after transplantation into neonatal mouse cortex. Although the dorsal MGE (dMGE)-SST and ventral MGE (vMGE)-PV bias were confirmed, both subgroups originate from progenitors located throughout the MGE. The dMGE bias was also found for SST subgroups that coexpress calretinin or reelin. In contrast, another major subgroup of SST interneuron, neuropeptide Y-expressing, does not appear to originate within the MGE. Finally, novel evidence is provided that a clinically important subtype of PV-expressing interneuron, the chandelier (axo-axonic) cell, is greatly enriched in transplants from the vMGE at embryonic day 15. These findings have important implications both for the study of interneuron fate determination and for studies that use interneuron precursor transplantation to alter cortical activity.
authors.affiliation: Department of Psychiatry, Weill Medical College of Cornell University, New York, NY 10021, USA.
authors.forename: Melis
authors.initials: M
authors.lastname: Inan
authors.forename: Jelle
authors.initials: J
authors.lastname: Welagen
authors.forename: Stewart A
authors.initials: SA
authors.lastname: Anderson
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D001973:Bromodeoxyuridine / Q000008:administration & dosage / Q000378:metabolism; D002452:Cell Count; D002454:Cell Differentiation; D004622:Embryo, Mammalian; D053595:Embryonic Stem Cells / Q000166:cytology / Q000637:transplantation; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007395:Interneurons / Q000145:classification / Q000378:metabolism / Q000637:transplantation; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D019579:Neocortex / Q000166:cytology / Q000378:metabolism / Q000637:transplantation; D009419:Nerve Tissue Proteins / Q000378:metabolism; D010320:Parvalbumins / Q000378:metabolism*; D011247:Pregnancy; D000091042:Reelin Protein; D013004:Somatostatin / Q000378:metabolism*; D013687:Telencephalon / Q000166:cytology* / Q000196:embryology; D013997:Time Factors
chemical_list: D009419:Nerve Tissue Proteins; D010320:Parvalbumins; D000091042:Reelin Protein; D049452:Green Fluorescent Proteins; D013004:Somatostatin; C000717128:Reln protein, mouse; D001973:Bromodeoxyuridine