title: Effect of hydrophilicity of carbon nanotube arrays on the release rate and activity of recombinant human bone morphogenetic protein-2.
abstract: Novel nanostructures such as vertically aligned carbon nanotube (CNT) arrays have received increasing interest as drug delivery carriers. In the present study, two CNT arrays with extreme surface wettabilities are fabricated and their effects on the release of recombinant human bone morphogenetic protein-2 (rhBMP-2) are investigated. It is found that the superhydrophilic arrays retained a larger amount of rhBMP-2 than the superhydrophobic ones. Further use of a poloxamer diffusion layer delayed the initial burst and resulted in a greater total amount of rhBMP-2 released from both surfaces. In addition, rhBMP-2 bound to the superhydrophilic CNT arrays remained bioactive while they denatured on the superhydrophobic surfaces. These results are related to the combined effects of rhBMP-2 molecules interacting with poloxamer and the surface, which could be essential in the development of advanced carriers with tailored surface functionalities.
authors.affiliation: Plasma Nanoscience Centre Australia (PNCA), CSIRO Materials Science and Engineering, Lindfield, New South Wales, Australia. zhaojun.han@csiro.au
authors.forename: Zhao Jun
authors.initials: ZJ
authors.lastname: Han
authors.forename: Kostya Ken
authors.initials: KK
authors.lastname: Ostrikov
authors.forename: Cher Ming
authors.initials: CM
authors.lastname: Tan
authors.forename: Beng Kang
authors.initials: BK
authors.lastname: Tay
authors.forename: Sean A F
authors.initials: SA
authors.lastname: Peel
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000494:pharmacology*; D002460:Cell Line; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions / Q000187:drug effects*; D051379:Mice; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D011994:Recombinant Proteins / Q000494:pharmacology; D012825:Silicon / Q000737:chemistry; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D017155:Wettability / Q000187:drug effects
chemical_list: D055396:Bone Morphogenetic Protein 2; D037742:Nanotubes, Carbon; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C499469:recombinant human bone morphogenetic protein-2; D000469:Alkaline Phosphatase; D012825:Silicon