title: Cytotoxic effect of eugenol on the expression of molecular markers related to the osteogenic differentiation of human dental pulp cells.
abstract: The cytotoxic effect of eugenol on the expression of molecular markers related to the osteogenic differentiation of human dental pulp cells such as collagen synthesis and the expression of two osteogenesis-related genes, alkaline phosphatase (ALP) and bone sialoprotein (BSP), was studied using human dental pulp cells (D824 cells). Cellular growth and survival were decreased by treatment of cells with eugenol in a concentration-dependent manner. The incorporation rate of [(3)H] proline into the acid-insoluble fraction and the synthesis of type I-V collagens were also reduced by treatment of cells with eugenol in a concentration-dependent fashion. The mRNA expression of ALP was scarcely affected in cells exposed to eugenol, whereas the mRNA and protein expression of BSP was down-regulated depending on the concentrations of eugenol. The results suggest that because collagen synthesis and BSP expression play a critical role in hard tissue formation, eugenol used for endodontic treatment may give rise to cytotoxic effects to the normal function of stem cells reported to exist in human dental pulp tissue and periodontal ligament.
authors.affiliation: Department of Pharmacology, The Nippon Dental University School of Life Dentistry at Tokyo, 1-9-20 Fujimi, Chiyoda-ku, Tokyo, 102-8159, Japan.
authors.forename: Masanori
authors.initials: M
authors.lastname: Anpo
authors.forename: Kumiko
authors.initials: K
authors.lastname: Shirayama
authors.forename: Takeki
authors.initials: T
authors.lastname: Tsutsui
mesh_terms: D000469:Alkaline Phosphatase / Q000187:drug effects*; D015153:Blotting, Western; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000187:drug effects*; D003764:Dental Materials / Q000633:toxicity*; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation; D005054:Eugenol / Q000008:administration & dosage / Q000633:toxicity*; D005260:Female; D006801:Humans; D058574:Integrin-Binding Sialoprotein / Q000187:drug effects*; D008422:Materials Testing; D010012:Osteogenesis / Q000187:drug effects*; D011392:Proline; D012333:RNA, Messenger / Q000187:drug effects; D019275:Radiopharmaceuticals; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014316:Tritium; D055815:Young Adult
chemical_list: D003764:Dental Materials; D058574:Integrin-Binding Sialoprotein; D012333:RNA, Messenger; D019275:Radiopharmaceuticals; D014316:Tritium; D005054:Eugenol; D003094:Collagen; D011392:Proline; D000469:Alkaline Phosphatase