title: Characterization of alginase and elicitor-active oligosaccharides from Gracilibacillus A7 in alleviating salt stress for Brassica campestris L.
abstract: Alginase was purified from Gracilibacillus A7 and evaluated for its ability to produce elicitor-active oligosaccharides. The optimum conditions for the alginase reaction are as follows: temperature, 40 °C; pH, 8.0; alginate content, 0.3-0.7%; and the presence of Na(+) and Mg(2+) metal ions. The degree of polymerization (DP) decreased as the reaction time of the alginase progressed, achieving values of 5.4 and 3.3 after 240 and 300 min, respectively. The relative root length (RRL) of the Brassica campestris L. increased with the addition of oligosaccharides with reduced DP values. The oligosaccharides with lower DP values are effective in reducing the effect of salt stress on the activity of the superoxide dismutase (SOD) and guaiacol peroxidase (POD), and oligosaccharides with moderate DP values can reduce the increase in lipid peroxidation activities (as malondialdehyde content) induced by salt stress. These results suggest that oligosaccharides may act as osmoprotective agents during the plant germination process.
authors.affiliation: College of Environmental Science and Engineering , Nankai University, Tianjin 300071, China.
authors.forename: Jingchun
authors.initials: J
authors.lastname: Tang
authors.forename: Qixing
authors.initials: Q
authors.lastname: Zhou
authors.forename: Hongrui
authors.initials: H
authors.lastname: Chu
authors.forename: Shinichi
authors.initials: S
authors.lastname: Nagata
mesh_terms: D001406:Bacillaceae / Q000737:chemistry / Q000201:enzymology* / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D001937:Brassica / Q000254:growth & development / Q000382:microbiology* / Q000502:physiology*; D007700:Kinetics; D009844:Oligosaccharides / Q000737:chemistry* / Q000378:metabolism; D058105:Polymerization; D011133:Polysaccharide-Lyases / Q000737:chemistry* / Q000378:metabolism; D012492:Salts / Q000378:metabolism; D013312:Stress, Physiological
chemical_list: D001426:Bacterial Proteins; D009844:Oligosaccharides; D012492:Salts; D011133:Polysaccharide-Lyases; C016158:poly(beta-D-mannuronate) lyase