title: Genomic and molecular analysis of phage CMP1 from Clavibacter michiganensis subspecies michiganensis.
abstract: Bacteriophage CMP1 is a member of the Siphoviridae family that infects specifically the plant-pathogen Clavibacter michiganensis subsp. michiganensis. The linear double- stranded DNA is terminally redundant and not circularly permuted. The complete nucleotide sequence of the bacteriophage CMP1 genome consists of 58,652 bp including the terminal redundant ends of 791 bp. The G+C content of the phage (57%) is significantly lower than that of its host (72.66%). 74 potential open reading frames were identified and annotated by different bioinformatic tools. Two large clusters which encode the early and the late functions could be identified which are divergently transcribed. There are only a few hypothetical gene products with conserved domains and significant similarity to sequences from the databases. Functional analyses confirmed the activity of four gene products, an endonuclease, an exonuclease, a single-stranded DNA binding protein and a thymidylate synthase. Partial genomic sequences of CN77, a phage of Clavibacter michiganensis subsp. nebraskensis, revealed a similar genome structure and significant similarities on the level of deduced amino acid sequences. An endolysin with peptidase activity has been identified for both phages, which may be good tools for disease control of tomato plants against Clavibacter infections.
authors.affiliation: Department of Microbiology/Genetechnology; Faculty of Biology; University of Bielefeld Universitaetsstr; Bielefeld, Germany.
authors.forename: Johannes
authors.initials: J
authors.lastname: Wittmann
authors.forename: Karl-Heinz
authors.initials: KH
authors.lastname: Gartemann
authors.forename: Rudolf
authors.initials: R
authors.lastname: Eichenlaub
authors.forename: Brigitte
authors.initials: B
authors.lastname: Dreiseikelmann