title: [The comparison between the vascular endothelial cells special cdc42-deficient heterozygous mice and the non-knockout mice on lung tissue pathological change and vasopermeability in acute lung injury].
abstract: OBJECTIVE
  To compare the change of lung tissue and vasopermeability between the vascular endothelial cells special cdc42-deficient heterozygous mice and the non-knockout mice in acute lung injury.
  
  
  METHODS
  The mice with vascular endothelial cell-specific expression of cre recombinase were crossed with cdc42(flox/flox) mice. The cdc42(flox/+)Cre(+/-) F1 offspring mice were crossed back with cdc42(flox/flox) mice, resulting in the F2 generation mice with three genotypes, namely cdc42(flox/+)Cre(+/-), cdc42(flox/flox)Cre(-/-) and cdc42(flox/+)Cre(+/-). The heterozygous mice with cdc42(flox/+)Cre(+/-) genotype were selected as the model mice, with the other two genotype groups as the control. After intratracheal instillation of 2 mg/kg LPS to induce acute lung injury, the mice were sacrificed to examine the lung pathologies, lung wet/dry ratio and lung microvascular permeability.
  
  
  RESULTS
  The heterozygous mice with cdc42 gene knockout (cdc42(flox/+)Cre(+/-)) showed no significant differences from the two control groups in the lung pathological score, lung wet/dry ratio or the lung microvascular permeability coefficient.
  
  
  CONCLUSIONS
  There were no significant difference on lung tissue and vasopermeability between the vascular endothelial cells special cdc42-deficient heterozygous mice and the non-knockout mice.
authors.affiliation: Department of Respiratory Disease, Nangfang Hospital, Southern Medical University, Guangzhou 510515, China. 956324887@qq.com
authors.forename: Guo-dong
authors.initials: GD
authors.lastname: Hu
authors.forename: Ying-hua
authors.initials: YH
authors.lastname: Chen
authors.forename: Wan-cheng
authors.initials: WC
authors.lastname: Tong
authors.forename: Yuan-xiong
authors.initials: YX
authors.lastname: Cheng
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Shao-xi
authors.initials: SX
authors.lastname: Cai
mesh_terms: D055371:Acute Lung Injury / Q000473:pathology*; D000818:Animals; D002199:Capillary Permeability; D042783:Endothelial Cells / Q000473:pathology; D019426:Integrases / Q000235:genetics; D008168:Lung / Q000098:blood supply / Q000473:pathology*; D051379:Mice; D018345:Mice, Knockout; D020764:cdc42 GTP-Binding Protein / Q000235:genetics
chemical_list: C045073:Cre recombinase; D019426:Integrases; D020764:cdc42 GTP-Binding Protein