title: Nacre-driven water-soluble factors promote wound healing of the deep burn porcine skin by recovering angiogenesis and fibroblast function.
abstract: To assess the recovery effect of water-soluble components of nacre on wound healing of burns, water-soluble nacre (WSN) was obtained from powdered nacre. Alterations to WSN-mediated wound healing characteristics were examined in porcine skin with deep second-degree burns; porcine skin was used as a proxy for human. When WSN was applied to a burned area, the burn-induced granulation sites were rapidly filled with collagen, and the damaged dermis and epidermis were restored to the appearance of normal skin. WSN enhanced wound healing recovery properties for burn-induced apoptotic and necrotic cellular damage and spurred angiogenesis. Additionally, WSN-treated murine fibroblast NIH3T3 cells showed increased proliferation and collagen synthesis. Collectively, the findings indicate that WSN improves the process of wound healing in burns by expeditiously restoring angiogenesis and fibroblast activity. WSN may be useful as a therapeutic agent, with superior biocompatibility to powdered nacre, and evoking less discomfort when applied to a wounded area.
authors.affiliation: Department of Microbiology, Yeungnam University College of Medicine, Daegu 705-717, Korea.
authors.forename: Kyunghee
authors.initials: K
authors.lastname: Lee
authors.forename: Hyunsoo
authors.initials: H
authors.lastname: Kim
authors.forename: Jin Man
authors.initials: JM
authors.lastname: Kim
authors.forename: Yeoun Ho
authors.initials: YH
authors.lastname: Chung
authors.forename: Tae Yoon
authors.initials: TY
authors.lastname: Lee
authors.forename: Hyun-Sook
authors.initials: HS
authors.lastname: Lim
authors.forename: Ji-Hye
authors.initials: JH
authors.lastname: Lim
authors.forename: Taewoon
authors.initials: T
authors.lastname: Kim
authors.forename: Jin Seung
authors.initials: JS
authors.lastname: Bae
authors.forename: Chang-Hoon
authors.initials: CH
authors.lastname: Woo
authors.forename: Keuk-Jun
authors.initials: KJ
authors.lastname: Kim
authors.forename: Daewon
authors.initials: D
authors.lastname: Jeong
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002056:Burns / Q000503:physiopathology*; D049109:Cell Proliferation / Q000187:drug effects; D003094:Collagen / Q000096:biosynthesis; D017931:DNA Primers / Q000235:genetics; D005347:Fibroblasts / Q000187:drug effects* / Q000502:physiology; D051379:Mice; D041681:NIH 3T3 Cells; D060734:Nacre / Q000737:chemistry / Q000494:pharmacology*; D018919:Neovascularization, Physiologic / Q000187:drug effects* / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000293:injuries*; D012995:Solubility; D034421:Sus scrofa; D014867:Water / Q000737:chemistry; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
chemical_list: D017931:DNA Primers; D060734:Nacre; D014867:Water; D003094:Collagen