title: Taurine inhibits serum deprivation-induced osteoblast apoptosis via the taurine transporter/ERK signaling pathway.
abstract: Taurine has positive effects on bone metabolism. However, the effects of taurine on osteoblast apoptosis in vitro have not been reported. The aim of this study was to investigate the activity of taurine on apoptosis of mouse osteoblastic MC3T3-E1 cells. The data showed that 1, 5, 10, or 20 mM taurine resulted in 16.7, 34.2, 66.9, or 63.75% reduction of MC3T3-E1 cell apoptosis induced by the serum deprivation (serum-free α-MEM), respectively. Taurine (1, 5, or 10 mM) also reduced cytochrome c release and inhibited activation of caspase-3 and -9, which were measured using fluorogenic substrates for caspase-3/caspase-9, in serum-deprived MC3T3-E1 cells. Furthermore, taurine (10 mM) induced extracellular signal-regulated kinase (ERK) phosphorylation in MC3T3-E1 cells. Knockdown of the taurine transporter (TAUT) or treatment with the ERK-specific inhibitor PD98059 (10 μM) blocked the activation of ERK induced by taurine (10 mM) and abolished the anti-apoptotic effect of taurine (10 mM) in MC3T3-E1 cells. The present results demonstrate for the first time that taurine inhibits serum deprivation-induced osteoblast apoptosis via the TAUT/ERK signaling pathway.
authors.affiliation: Department of Spine Surgery, Second Xiangya Hospital, Central South University, Changsha, Hunan, China.
authors.forename: Lei-Yi
authors.initials: LY
authors.lastname: Zhang
authors.forename: Yue-Ying
authors.initials: YY
authors.lastname: Zhou
authors.forename: Fei
authors.initials: F
authors.lastname: Chen
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: You-Wen
authors.initials: YW
authors.lastname: Deng
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Liu
authors.forename: Zheng-Guang
authors.initials: ZG
authors.lastname: Wang
authors.forename: Ya-Wei
authors.initials: YW
authors.lastname: Li
authors.forename: Dong-Zhe
authors.initials: DZ
authors.lastname: Li
authors.forename: Guo-Hua
authors.initials: GH
authors.lastname: Lv
authors.forename: Bang-Liang
authors.initials: BL
authors.lastname: Yin
mesh_terms: D016475:3T3 Cells; D000704:Analysis of Variance; D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology*; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D002417:Cattle; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000378:metabolism*; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D013654:Taurine / Q000494:pharmacology*; D000099247:Taurine Transporters
chemical_list: D053148:Caspase 3; D053453:Caspase 9; D048049:Extracellular Signal-Regulated MAP Kinases; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D012333:RNA, Messenger; D013654:Taurine; D000099247:Taurine Transporters