title: Construction, expression, and purification of chimeric protein reagents based on immunoglobulin fc regions.
abstract: Recombinant fusion proteins incorporating experimental protein domains fused to immunoglobulin Fc regions have become widely utilized in studies of protein-ligand interactions. The advantages of these systems include an inherent increase in avidity provided by the multimerization of Fc regions, combined with robust detection methods based on numerous commercially available secondary reagents directed against the Fc tag. We describe a set of methods for subcloning, expression, and purification of chimeric protein reagents containing a protein domain (or domains) of interest fused to a C-terminal moiety derived from the Fc region of either IgG or IgM.
authors.affiliation: Department of Pediatrics, University of South Florida College of Medicine, University of South Florida and All Children's Hospital Children's Research Institute, St. Petersburg, FL, USA. jcannon@health.usf.edu
authors.forename: John P
authors.initials: JP
authors.lastname: Cannon
authors.forename: Marci
authors.initials: M
authors.lastname: O'Driscoll
authors.forename: Gary W
authors.initials: GW
authors.lastname: Litman
mesh_terms: D019921:Biotinylation; D015153:Blotting, Western; D002460:Cell Line; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D007141:Immunoglobulin Fc Fragments; D011993:Recombinant Fusion Proteins