title: Inhibitor of DNA-binding-1/inhibitor of differentiation-1 (ID-1) is implicated in various aspects of gastric cancer cell biology.
abstract: In order to determine the biological roles of the inhibitor of DNA-binding-1/inhibitor of differentiation-1 (ID-1) protein in MGC803 and AGS cell lines, we ectopically expressed or downregulated ID-1 in the both gastric cell lines and measured various parameters of tumor cell development, including cell proliferation, cell cycle progression, apoptosis and cell migration. The ectopic expression of ID-1 significantly enhanced cell proliferation, cell cycle progression and cell migration, and protected MGC803 and AGS cell lines from cisplatin-induced apoptosis. The opposite effects were observed after downregulation of ID-1, which in combination with cisplatin treatment enhanced apoptosis in a synergistic fashion. Collectively, these findings demonstrate that ID-1 plays pivotal and diverse roles in the biology of certain gastric cancer cells, further suggesting that ID-1 is implicated in the pathogenesis and progression of gastric cancer.
authors.affiliation: Biomedical Engineering Institute, School of Control Science and Engineering, Shandong University, Jinan, 250021 Shandong, China. cindy@sdu.edu.cn
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Chun-Hua
authors.initials: CH
authors.lastname: Zhang
authors.forename: Yi-Lin
authors.initials: YL
authors.lastname: Hong
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Yong-Mei
authors.initials: YM
authors.lastname: Hu
authors.forename: Cui-Fen
authors.initials: CF
authors.lastname: Zhao
mesh_terms: D017209:Apoptosis / Q000235:genetics; D015153:Blotting, Western; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D002945:Cisplatin; D005434:Flow Cytometry; D005453:Fluorescence; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D051798:Inhibitor of Differentiation Protein 1 / Q000235:genetics* / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics; D013274:Stomach Neoplasms / Q000235:genetics*
chemical_list: C497453:ID1 protein, human; D051798:Inhibitor of Differentiation Protein 1; D034741:RNA, Small Interfering; D002945:Cisplatin