title: Isothiocyanates from Broccolini seeds induce apoptosis in human colon cancer cells: proteomic and bioinformatic analyses.
abstract: Isothiocyanates (ITCs) have been shown to possess antitumor activity in colon cancer, however, the detailed mechanism is still unclear. The objective of this study was to investigate apoptosis-inducing activity of ITCs from Broccolini seeds and proteomic changes in SW480 cells, and to identify the molecular pathways responsible for the anticancer action of ITCs. We found that ITCs induces SW480 cells apoptosis in a dose-dependent manner by using MTT assay, phase contrast microscope and flow cytometry, and the IC50 was calculated to be 77.72 microg/ml, superior to the chemotherapeutical drug 5-flurouracil. Subsequently, 15 altered proteins in ITCs treated SW480 cells were identified. Further bioinformatics analysis predicted the potential pathways for ITCs to induce apoptosis of SW480 cells. In conclusion, this is the first report to investigate anticancer activity of ITCs from Broccolini seeds and its mechanism of action by proteomics analysis. Our observations provide potential therapeutic targets for colon cancer inhibitor intervention and implicate the development of novel anti-cancer therapeutic strategies.
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou China.
authors.forename: Yanjing
authors.initials: Y
authors.lastname: Yang
authors.forename: Huidan
authors.initials: H
authors.lastname: Yan
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Li
authors.forename: Shang-Tian
authors.initials: ST
authors.lastname: Yang
authors.forename: Xuewu
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001937:Brassica / Q000737:chemistry*; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000188:drug therapy* / Q000473:pathology; D004396:Coloring Agents; D019295:Computational Biology; D015180:Electrophoresis, Gel, Two-Dimensional; D005434:Flow Cytometry; D006801:Humans; D007091:Image Processing, Computer-Assisted; D017879:Isothiocyanates / Q000494:pharmacology*; D008969:Molecular Sequence Data; D011492:Protein Hydrolysates / Q000737:chemistry; D011506:Proteins / Q000737:chemistry; D040901:Proteomics; D012639:Seeds / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013778:Tetrazolium Salts; D013844:Thiazoles; D014357:Trypsin / Q000737:chemistry
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004396:Coloring Agents; D017879:Isothiocyanates; D011492:Protein Hydrolysates; D011506:Proteins; D013778:Tetrazolium Salts; D013844:Thiazoles; D014357:Trypsin; C022616:thiazolyl blue