title: Initial analysis of the hemocyanin subunit type 1 (Hc1 gene) from Locusta migratoria manilensis.
abstract: Hemocyanins are copper-containing (Cu(+)) proteins that transport oxygen in many arthropods hemolymph. We characterized Hc1 gene from the grasshopper species Locusta migratoria manilensis. In particular, we cloned and sequenced the corresponding cDNAs and studied their expression at different developmental stages. The cDNA of Hc1 gene (GenBank accession no.:HQ213937) is 2271 bp in length and the open reading frame is 2016 bp, which encodes a 672 amino acids protein with a calculated molecular mass of 77.9 kD and the isoelectric point of 6.06. Sequence alignment analysis result showed that this gene shares 94.7% identity with Schistocerca americana EHP. In addition, analysis of quantitative RT-PCR indicated that, LmiHc1 was expressed in the embyro (24, 39, 62, 86, 144, and 193 h after hatch), nymphs (1st instar, 2nd instar, 3rd instar, 4th instar and 5th instar) and in adult. These results showed that Hc1 plays an important role in grasshopper, which may be related to an enhanced oxygen supply. Phylogenetic analysis of insecta based on Hc1 are basically consistent with the morphology.
authors.affiliation: College of Life Sciences, Hebei University, 071002 Baoding, People's Republic of China.
authors.forename: Hong
authors.initials: H
authors.lastname: Yin
authors.forename: Ni
authors.initials: N
authors.lastname: Guan
authors.forename: Lijun
authors.initials: L
authors.lastname: Dong
authors.forename: Qiaoyun
authors.initials: Q
authors.lastname: Yue
authors.forename: Xiangchu
authors.initials: X
authors.lastname: Yin
authors.forename: Daochuan
authors.initials: D
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001499:Bayes Theorem; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004625:Embryo, Nonmammalian / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D006433:Hemocyanins / Q000235:genetics*; D019476:Insect Proteins / Q000235:genetics*; D008018:Life Cycle Stages / Q000235:genetics; D048188:Locusta migratoria / Q000235:genetics* / Q000254:growth & development; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010802:Phylogeny; D060888:Real-Time Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D019476:Insect Proteins; D006433:Hemocyanins