title: Increased mass levels of certain serine proteases in the stratum corneum in acute eczematous atopic skin.
abstract: Acute eczematous atopic dermatitis (AD) is associated with increases in stratum corneum (SC) serine protease activity. The purpose of this study was to examine whether the increased SC protease activities in acute eczematous atopic dermatitis were associated with increased mass levels of SC proteases. Six subjects with healthy skin and six patients with AD each with non-lesional skin or lesional acute eczematous skin had the mass levels of their extractable SC kallikreins (KLK), plasmin and urokinase quantified using Luminex multiplex bead-based assays from SC tape strippings. The mass levels of KLK5 and KLK14 together with urokinase were not elevated in the SC in atopic skin. However, the mass levels of KLK7 and KLK11 together with plasmin were greatly elevated compared with the extracts from the non-lesional and the healthy skin and correlated with the corresponding enzymatic activities.
authors.affiliation: DSM Nutritional Products Ltd, Basel, Switzerland. rainer.voegeli@dsm.com
authors.forename: R
authors.initials: R
authors.lastname: Voegeli
authors.forename: S
authors.initials: S
authors.lastname: Doppler
authors.forename: P
authors.initials: P
authors.lastname: Joller
authors.forename: M
authors.initials: M
authors.lastname: Breternitz
authors.forename: J W
authors.initials: JW
authors.lastname: Fluhr
authors.forename: A V
authors.initials: AV
authors.lastname: Rawlings
mesh_terms: D000328:Adult; D003876:Dermatitis, Atopic / Q000201:enzymology*; D005341:Fibrinolysin / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D007610:Kallikreins / Q000378:metabolism*; D014568:Urokinase-Type Plasminogen Activator / Q000378:metabolism*; D055815:Young Adult
chemical_list: D007610:Kallikreins; D005341:Fibrinolysin; D014568:Urokinase-Type Plasminogen Activator