title: Evaluation of matrix-assisted laser desorption ionization-time of flight mass spectrometry for rapid identification of Beta-hemolytic streptococci.
abstract: This study was undertaken to evaluate matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for the rapid identification of beta-hemolytic streptococci. We compared Bruker Biotyper 2.0 with Vitek2 coupled to the agglutination test. MALDI-TOF MS analysis of 386 beta-hemolytic streptococcal isolates yielded high-confidence identification to the species level for all 386 isolates. The Vitek2 gave high-confidence identification to the species level for 88% of Streptococcus agalactiae isolates (n = 269/306), 92% of Streptococcus pyogenes isolates (n = 48/52), and 39% of isolates of Streptococcus dysgalactiae serogroups C and G (n = 11/28).
authors.affiliation: Clinical Microbiology Laboratory, Service of Infectious Diseases, University of Geneva Hospitals (HUG), CH-1211 Geneva 14, Switzerland. abdessalam.cherkaoui@hcuge.ch
authors.forename: Abdessalam
authors.initials: A
authors.lastname: Cherkaoui
authors.forename: Stéphane
authors.initials: S
authors.lastname: Emonet
authors.forename: José
authors.initials: J
authors.lastname: Fernandez
authors.forename: Didier
authors.initials: D
authors.lastname: Schorderet
authors.forename: Jacques
authors.initials: J
authors.lastname: Schrenzel
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D006801:Humans; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013290:Streptococcal Infections / Q000175:diagnosis* / Q000382:microbiology; D013291:Streptococcus / Q000737:chemistry* / Q000145:classification* / Q000302:isolation & purification