title: A narrow microbeam is more effective for tumor growth suppression than a wide microbeam: an in vivo study using implanted human glioma cells.
abstract: The tumoricidal mechanisms of microbeam radiation therapy, and the more recently proposed minibeam radiation therapy, for the treatment of brain tumors are as yet unclear. Moreover, from among the various parameters of beam geometry the impact of changing the beam width is unknown. In this study, suppression of tumor growth in human glioma cells implanted in mice was evaluated experimentally using microbeams of two different widths: a conventional narrow beam (20 µm width, 100 µm center-to-center distance) and a wide beam (100 µm width, 500 µm center-to-center distance). The tumor growth ratio was compared and acute cell death was studied histologically. With cross-planar irradiation, tumor growth was significantly suppressed between days 4 and 28 after 20 µm microbeam irradiation, whereas tumor growth was suppressed, and not significantly so, only between days 4 and 18 after 100 µm microbeam irradiation. Immunohistochemistry using TUNEL staining showed no increase in TUNEL-positive cells with either microbeam at 24 and 72 h post-irradiation. The 20 µm microbeam was found to be more tumoricidal than the 100 µm microbeam, and the effect was not related to apoptotic cell death. The underlying mechanism may be functional tissue deterioration rather than direct cellular damage in the beam path.
authors.affiliation: Department of Neurosurgery, Kobe University Graduate School of Medicine, Kobe, Hyogo, Japan. auyama811@yahoo.co.jp
authors.forename: Atsushi
authors.initials: A
authors.lastname: Uyama
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kondoh
authors.forename: Nobuteru
authors.initials: N
authors.lastname: Nariyama
authors.forename: Keiji
authors.initials: K
authors.lastname: Umetani
authors.forename: Manabu
authors.initials: M
authors.lastname: Fukumoto
authors.forename: Kunio
authors.initials: K
authors.lastname: Shinohara
authors.forename: Eiji
authors.initials: E
authors.lastname: Kohmura
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000473:pathology*; D002455:Cell Division / Q000528:radiation effects*; D004195:Disease Models, Animal; D005910:Glioma / Q000473:pathology*; D051379:Mice