title: Protein stability in an ionic liquid milieu: on the use of differential scanning fluorimetry.
abstract: Protein stability is a major bottleneck in the biotechnological application of ionic liquid-containing solvents, either in the frame of biocatalysis or protein storage. Herein, differential scanning fluorimetry was successfully implemented as a high throughput method to fast scan the impact of a number of cholinium-based ionic liquids on the stability of proteins.
authors.affiliation: Instituto Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av. República 127, 2780-756 Oeiras, Portugal.
authors.forename: João V
authors.initials: JV
authors.lastname: Rodrigues
authors.forename: Vesna
authors.initials: V
authors.lastname: Prosinecki
authors.forename: Isabel
authors.initials: I
authors.lastname: Marrucho
authors.forename: Luís Paulo N
authors.initials: LP
authors.lastname: Rebelo
authors.forename: Cláudio M
authors.initials: CM
authors.lastname: Gomes
mesh_terms: D005470:Fluorometry; D052578:Ionic Liquids / Q000737:chemistry*; D009113:Muramidase / Q000737:chemistry; D044367:Phase Transition; D055550:Protein Stability; D011506:Proteins / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry
chemical_list: D052578:Ionic Liquids; D011506:Proteins; D012997:Solvents; D009113:Muramidase