title: From the genome sequence to the protein inventory of Bacillus subtilis.
abstract: Owing to the low number of proteins necessary to render a bacterial cell viable, bacteria are extremely attractive model systems to understand how the genome sequence is translated into actual life processes. One of the most intensively investigated model organisms is Bacillus subtilis. It has attracted world-wide research interest, addressing cell differentiation and adaptation on a molecular scale as well as biotechnological production processes. Meanwhile, we are looking back on more than 25 years of B. subtilis proteomics. A wide range of methods have been developed during this period for the large-scale qualitative and quantitative proteome analysis. Currently, it is possible to identify and quantify more than 50% of the predicted proteome in different cellular subfractions. In this review, we summarize the development of B. subtilis proteomics during the past 25 years.
authors.affiliation: Institute for Microbiology, Ernst Moritz Arndt University Greifswald, Greifswald, Germany. dbecher@uni-greifswald.de
authors.forename: Dörte
authors.initials: D
authors.lastname: Becher
authors.forename: Knut
authors.initials: K
authors.lastname: Büttner
authors.forename: Martin
authors.initials: M
authors.lastname: Moche
authors.forename: Bernd
authors.initials: B
authors.lastname: Hessling
authors.forename: Michael
authors.initials: M
authors.lastname: Hecker
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D016680:Genome, Bacterial; D040901:Proteomics
chemical_list: D001426:Bacterial Proteins