title: Comparative chromosome painting of four Siberian Vespertilionidae species with Aselliscus stoliczkanus and human probes.
abstract: Vespertilionidae is the largest chiropteran family that comprises species of different specialization and wide geographic distribution. Up to now, only a few vespertilionid species have been studied by molecular cytogenetic approaches. Here, we have investigated the karyotypic relationships of 4 Vespertilionidae species from Siberia by G-banding and comparative chromosome painting. Painting probes from Aselliscus stoliczkanus were used to establish interspecific homologous chromosomal segments in Myotis dasycneme (2n = 44), Murina hilgendorfi (2n = 44), Plecotus auritus (2n = 32), and Vespertilio murinus (2n = 38). Robertsonian translocations and a few inversions differentiated the karyotypes of the examined species. Painting of P. auritus karyotype with human probes revealed 3 previously undetected cryptic segments homologous to human chromosomes (Homo sapiens, HSA) 8, 15, and 19, respectively. As a consequence, the existence of 2 HSA 4 + 8 syntenies in the P. auritus karyotype has been proven. In addition, a pericentric inversion or centromere shift was revealed on the smallest metacentric P. auritus chromosome 16/17 using the HSA 16 probe explaining the different G-banding pattern in comparison to the homologous Myotis chromosome 16/17.
authors.affiliation: Molecular and Cellular Biology Department, Institute of Chemical Biology and Fundamental Medicine, SÐ' RAS, Novosibirsk, Russia. zakal@mcb.nsc.ru
authors.forename: A I
authors.initials: AI
authors.lastname: Kulemzina
authors.forename: W
authors.initials: W
authors.lastname: Nie
authors.forename: V A
authors.initials: VA
authors.lastname: Trifonov
authors.forename: Y
authors.initials: Y
authors.lastname: Staroselec
authors.forename: D A
authors.initials: DA
authors.lastname: Vasenkov
authors.forename: M
authors.initials: M
authors.lastname: Volleth
authors.forename: F
authors.initials: F
authors.lastname: Yang
authors.forename: A S
authors.initials: AS
authors.lastname: Graphodatsky
mesh_terms: D000818:Animals; D002460:Cell Line; D002685:Chiroptera / Q000235:genetics*; D002871:Chromosome Banding; D020223:Chromosome Painting; D002877:Chromosomes, Human; D019143:Evolution, Molecular; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D012800:Siberia