title: [Influence of a glucocorticoid kenalog on functional activity of macrophages and neutrophils both in lungs and in peritoneal cavity].
abstract: The work purpose was comparative studying the number change and functional activity of macrophages and neutrophils both in lungs and in peritoneal cavity under the influence of a synthetic glucocorticoid kenalog (Kn). Kn was entered to rats Wistar unitary subcutaneously in a dose of 2 mg/kg (experience), the control rats obtained similarly 0.9% solution NaCl. The cell number of rat bronchoalveolar lavage liquid (BALL) and peritoneal lavage liquid (PLL) was defined in different terms after that. In cell monolayers of BALL we estimated ability of alveolar macrophages (AMph) and neutrophils to engulf of Staphylococcus and to generate of superoxide-anionradicals. In cell monolayers of PLL we estimated the functional activity of peritoneal macrophages (PMph) and neutrophils by means of the same tests. Through 6 (see symbol) after introduction Kn in BALL there was the reduction, and through 5 days--the increase of total cell number with the maximum of neutrophil accumulation and their considerable prevalence over AMph on number, phagocyte and superoxide-producing activity. At the same time the cell number in PLL was decreased and reached a minimum for 5 days after Kn-introduction with considerable decrease of phagocyte and superoxide-producing ability both PMph and neutrophils. The differing action of glucocorticoids on macrophages and neutrophils of different organism compartments and recruitment of considerable quantity of neutrophils, possessing in destructive potential, into lungs can serve as an explanation of many cases when high-dose glucocorticoid therapy is not effective and may be even harmful at attempts of preventing acute respiratory distress-syndrome.
authors.forename: L N
authors.initials: LN
authors.lastname: Shishkina
authors.forename: E M
authors.initials: EM
authors.lastname: Malkova
mesh_terms: D000818:Animals; D001992:Bronchoalveolar Lavage Fluid; D005260:Female; D005938:Glucocorticoids / Q000494:pharmacology*; D008168:Lung / Q000378:metabolism; D016676:Macrophages, Alveolar / Q000378:metabolism*; D017737:Macrophages, Peritoneal / Q000378:metabolism*; D009504:Neutrophils / Q000378:metabolism*; D010537:Peritoneum / Q000378:metabolism; D010587:Phagocytosis / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D012128:Respiratory Distress Syndrome / Q000188:drug therapy / Q000378:metabolism; D013210:Staphylococcus / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D014222:Triamcinolone Acetonide / Q000494:pharmacology*
chemical_list: D005938:Glucocorticoids; D013481:Superoxides; D014222:Triamcinolone Acetonide