title: Elevated frequency of CD1c+ myeloid dendritic cells in the peripheral blood mononuclear cells of simian/human immunodeficiency virus (SHIV) and simian immunodeficiency virus (SIV) repeatedly infected Chinese rhesus macaques.
abstract: CD1c+ myeloid dendritic cells (mDCs) in the peripheral blood of 30 SHIV-SF162p4 and SIVmac251 sequentially infected Chinese rhesus macaques were examined by flow cytometry to obtain further insight into mDC alterations in HIV/AIDS. The CD1c+ cells were found to be mononuclear leukocytes rather than granulocytes, and most of them expressed CD20. CD1c+mDCs (CD1c+CD20-) consisted of two morphological subsets: the granular and the large CD1c+mDCs. The expression of HLA-DR, CD86, and CD11b, but no CCR7, CD83 and CD123, together with their endocytotic capacity indicated that they were immature mDCs. Their frequency at weeks 10 and 12 post-infection was significantly higher than that of un-infected ones; the large CD1c+mDC level was significantly different between time points and almost absent from un-infected rhesus monkeys; significant correlations between CD1c+mDCs and plasma viral load levels were also observed. These data indicated a possible role for CD1c+mDCs in the pathophysiological process of SIV/HIV infection.
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Center for AIDS/STD Control and Prevention, China-CDC, Beijing 102206, PR China. guibyang@public.bta.net.cn
authors.forename: Gui-Bo
authors.initials: GB
authors.lastname: Yang
authors.forename: Na
authors.initials: N
authors.lastname: Lei
authors.forename: Chun-Miao
authors.initials: CM
authors.lastname: Zong
authors.forename: Jia-Zhong
authors.initials: JZ
authors.lastname: Duan
authors.forename: Hui
authors.initials: H
authors.lastname: Xing
authors.forename: Yiming
authors.initials: Y
authors.lastname: Shao
mesh_terms: D000818:Animals; D018949:Antigens, CD1 / Q000097:blood / Q000276:immunology*; D018951:Antigens, CD20 / Q000097:blood / Q000276:immunology; D051940:B7-2 Antigen / Q000097:blood / Q000276:immunology; D039481:CD11b Antigen / Q000097:blood / Q000276:immunology; D002681:China; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D004705:Endocytosis / Q000276:immunology; D005434:Flow Cytometry; D015658:HIV Infections / Q000097:blood / Q000276:immunology* / Q000821:virology; D006684:HLA-DR Antigens / Q000097:blood / Q000276:immunology; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology* / Q000378:metabolism / Q000821:virology; D008253:Macaca mulatta; D022423:Myeloid Cells / Q000276:immunology / Q000378:metabolism; D016097:Simian Acquired Immunodeficiency Syndrome / Q000097:blood / Q000276:immunology* / Q000821:virology
chemical_list: D018949:Antigens, CD1; D018951:Antigens, CD20; D051940:B7-2 Antigen; D039481:CD11b Antigen; D006684:HLA-DR Antigens