title: [Simultaneous assay of paeoniflorin and albiflorin in Paeoniae Radix Alba by QAMS].
abstract: OBJECTIVE
  To develop a method of quantitative analysis of multi-components by single marker (QAMS) for simultaneously determining paeoniflorin and albiflorin in Paeoniae Radix Alba.
  
  
  METHODS
  Using paeoniflorin as the internal reference substance, the relative correction factor (RCF) of albiflorin was determined by HPLC and UPLC with good reproducibility. The contents of paeoniflorin in 16 samples of Paeoniae Radix Alba were authentically determined by the external standard method, and the content of albiflorin was calculated according to the RCF. The contents of these two components in the samples were determined with the external standard method.
  
  
  RESULTS
  No siginificant differences between the quantitative results of QAMS method and external standard method were observe.
  
  
  CONCLUSIONS
  It is a convenient and accurate method to determine multi-components when some authentic standard substances were unavailable. It can be used to control the quality of Paeoniae Radix Alba
authors.affiliation: Shanghai University of Traditional Chinese Medicines, Shanghai 201203, China.
authors.forename: Shanjun
authors.initials: S
authors.lastname: Huang
authors.forename: Qiwei
authors.initials: Q
authors.lastname: Yang
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Shi
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.forename: Zhengtao
authors.initials: Z
authors.lastname: Wang
mesh_terms: D001565:Benzoates / Q000032:analysis*; D001952:Bridged-Ring Compounds / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005960:Glucosides / Q000032:analysis*; D039821:Monoterpenes; D029596:Paeonia / Q000737:chemistry*; D006027:Glycosides
chemical_list: D001565:Benzoates; D001952:Bridged-Ring Compounds; D005960:Glucosides; D039821:Monoterpenes; C015423:peoniflorin; D006027:Glycosides; C014959:albiflorin