title: Detection and genotyping of human papilloma virus in cervical cancer specimens from Saudi patients.
abstract: OBJECTIVE
  To determine the rates and types of human papilloma virus (HPV) infection in cervical cancer specimens from Saudi patients.
  
  
  METHODS
  One hundred specimens were randomly selected and retrieved from the achieved samples stored in the pathology department accessioned under the diagnosis of cervical cancer and carcinoma in situ between the years 1997 and 2007. Human papilloma virus in the clinical samples was detected using polymerase chain reaction amplification methods. Two primer systems are commonly used: the MY09-MY11 primers and the GP5+-GP6+ that amplify a wide range of HPV genotypes. Human papilloma virus isolates were genotyped using DNA sequencing and reverse line blot hybridization assay to identify the high-risk HPV genotypes.
  
  
  RESULTS
  Ninety cases fulfilled the diagnostic criteria and were analyzed. The rate of HPV genotype detection among cervical cancer samples was 95.5%. The most common HPV genotype detected by both methods was HPV-16 (63.4%), followed by HPV-18 (11.1%), HPV-45 (4.5%), HPV-33 (3.3%), and HPV-31, HPV-52, HPV-53, HPV-58, HPV-59, and HPV-66 with 2.2% prevalence rate each.
  
  
  CONCLUSIONS
  Prevalence of HPV genotypes among patients with cervical cancer in Saudi Arabia is comparable to the international rates. The use of the reverse line blot hybridization assay genotyping method could be useful for classifying oncogenic HPV-positive women. It is relatively inexpensive and reliable and can be performed in routine practice or epidemiological study compared with the available standard commercial kits.
authors.affiliation: King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia.
authors.forename: Ismail A
authors.initials: IA
authors.lastname: Al-Badawi
authors.forename: Abdulrahman
authors.initials: A
authors.lastname: Al-Suwaine
authors.forename: Murad
authors.initials: M
authors.lastname: Al-Aker
authors.forename: Lina
authors.initials: L
authors.lastname: Asaad
authors.forename: Alwaleed
authors.initials: A
authors.lastname: Alaidan
authors.forename: Asma
authors.initials: A
authors.lastname: Tulbah
authors.forename: Marie
authors.initials: M
authors.lastname: Fe Bohol
authors.forename: Adnan R
authors.initials: AR
authors.lastname: Munkarah
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D052685:Alphapapillomavirus / Q000235:genetics* / Q000302:isolation & purification*; D002294:Carcinoma, Squamous Cell / Q000453:epidemiology / Q000209:etiology / Q000235:genetics / Q000821:virology*; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D030361:Papillomavirus Infections / Q000150:complications / Q000175:diagnosis* / Q000453:epidemiology; D015995:Prevalence; D012529:Saudi Arabia / Q000453:epidemiology; D013048:Specimen Handling; D002583:Uterine Cervical Neoplasms / Q000453:epidemiology / Q000209:etiology / Q000235:genetics / Q000821:virology*; D055815:Young Adult
chemical_list: D004279:DNA, Viral