title: Expression of keratinocyte growth factor in goat ovaries and its effects on preantral follicles within cultured ovarian cortex.
abstract: The aims of this study were to evaluate the expression of keratinocyte growth factor (KGF) in goat ovaries and to study its effects on preantral follicle survival and development. The ovaries were used for immunohistochemistry or for in vitro culture for 1 or 7 days with KGF (0, 1, 10, 50, 100, 150, or 200 ng/mL). Noncultured (fresh control) and cultured ovarian slices were processed for histological analysis and transmission electron microscopy (TEM). The results showed that after 7 days of in vitro culture, all treatments had a significant reduction in the percentage of normal follicles compared with the fresh control. After 7 days of culture, the highest KGF concentrations (150 and 200 ng/mL) induced a significant reduction in the percentage of normal follicles compared with the tissues cultured in the absence (α-MEM(+) alone) or presence of 1, 10, and 50 ng/mL KGF. Transmission electron microscopy confirmed follicular integrity after 7 days of culture in 1 ng/mL KGF. In addition, compared with the fresh control, the percentage of growing follicles was significantly increased in all treatments after 1 or 7 days of culture. Immunohistochemical analyses showed the expression of KGF in oocytes and granulosa cells in all follicle developmental stages as well as in thecal and stromal cells. In conclusion, this study demonstrated that, at the lowest concentration (1 ng/mL), KGF maintained the ultrastructure of goat preantral follicles cultured in vitro for up to 7 days. Furthermore, the KGF protein was widely distributed in goat ovaries, especially in ovarian follicles.
authors.affiliation: Laboratory of Manipulation of Oocytes and Preantral Follicles, State University of Ceará, Fortaleza, CE, Brazil. lrfaustino@gmail.com
authors.forename: L R
authors.initials: LR
authors.lastname: Faustino
authors.forename: R
authors.initials: R
authors.lastname: Rossetto
authors.forename: I M T
authors.initials: IM
authors.lastname: Lima
authors.forename: C M G
authors.initials: CM
authors.lastname: Silva
authors.forename: M V A
authors.initials: MV
authors.lastname: Saraiva
authors.forename: L F
authors.initials: LF
authors.lastname: Lima
authors.forename: A W B
authors.initials: AW
authors.lastname: Silva
authors.forename: M A M
authors.initials: MA
authors.lastname: Donato
authors.forename: C C
authors.initials: CC
authors.lastname: Campello
authors.forename: C A
authors.initials: CA
authors.lastname: Peixoto
authors.forename: J R
authors.initials: JR
authors.lastname: Figueiredo
authors.forename: A P R
authors.initials: AP
authors.lastname: Rodrigues
mesh_terms: D000818:Animals; D002470:Cell Survival; D005260:Female; D051523:Fibroblast Growth Factor 7 / Q000378:metabolism*; D006041:Goats; D007150:Immunohistochemistry; D046529:Microscopy, Electron, Transmission; D006080:Ovarian Follicle / Q000378:metabolism* / Q000648:ultrastructure; D010053:Ovary / Q000378:metabolism* / Q000648:ultrastructure; D017154:Stromal Cells / Q000378:metabolism; D013997:Time Factors; D046509:Tissue Culture Techniques
chemical_list: D051523:Fibroblast Growth Factor 7