title: A new lectin from the tuberous rhizome of Kaempferia rotunda: isolation, characterization, antibacterial and antiproliferative activities.
abstract: A lectin (designated as KRL) was purified from the extracts of Kaempferia rotunda Linn. tuberous rhizome by glucose-sepharose affinity chromatography. KRL was determined to be a 29.0 ± 1.0 kDa polypeptide by SDS-PAGE under both reducing and non-reducing conditions. KRL was a divalent ion dependent glycoprotein with 4% neutral sugar which agglutinated different groups of human blood cells. Methyl-α-D-mannopyranoside, D-mannose and methyl-α-D-glucopyranoside were the most potent inhibitors. N-terminal sequence of KRL showed similarity to some mannose/ glucose specific lectins but the main differences with their molecular masses and sugar content. KRL lost its activity markedly in the presence of denaturants and exhibited high agglutination activity from pH 6.0 to 8.2 and temperature 30 to 60° C. The lectin showed toxicity against brine shrimp nauplii with the LC50 value of 18 ± 6 µg/ml and strong agglutination activity against seven pathogenic bacteria. KRL inhibited the growth of six bacteria partially and did not show antifungal activity. In addition, antiproliferative activity against Ehrlich ascites carcinoma (EAC) cells showed 51% and 67% inhibition in vivo in mice administered 1.25 mg/kg/day and 2.5 mg/kg/day of KRL respectively by injection for five days.
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Science, Rajshahi University, Rajshahi- 6205, Bangladesh. rashelkabir@gmail.com
authors.forename: Syed Rashel
authors.initials: SR
authors.lastname: Kabir
authors.forename: Amir
authors.initials: A
authors.lastname: Hossen
authors.forename: Abu
authors.initials: A
authors.lastname: Zubair
authors.forename: Jahangir
authors.initials: J
authors.lastname: Alom
authors.forename: Farhadul
authors.initials: F
authors.lastname: Islam
authors.forename: Anowar
authors.initials: A
authors.lastname: Hossain
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Kimura
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology / Q000633:toxicity; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology / Q000633:toxicity; D001156:Artemia / Q000187:drug effects; D001419:Bacteria / Q000187:drug effects / Q000254:growth & development; D050260:Carbohydrate Metabolism; D002413:Cations, Divalent / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002645:Chickens; D006384:Hemagglutination / Q000187:drug effects; D006801:Humans; D006863:Hydrogen-Ion Concentration; D051379:Mice; D008970:Molecular Weight; D037121:Plant Lectins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology* / Q000633:toxicity; D035281:Plant Tubers / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry; D011489:Protein Denaturation / Q000187:drug effects; D027343:Rhizome / Q000737:chemistry*; D017422:Sequence Analysis, DNA; D013379:Substrate Specificity; D013696:Temperature; D023041:Xenograft Model Antitumor Assays; D030521:Zingiberaceae / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D002413:Cations, Divalent; D037121:Plant Lectins