title: ppGpp in Sinorhizobium meliloti: biosynthesis in response to sudden nutritional downshifts and modulation of the transcriptome.
abstract: Sinorhizobium meliloti Rm2011 responds to sudden shifts to nitrogen or carbon starvation conditions by an accumulation of the stringent response alarmone ppGpp and remodelling of the transcriptome. The gene product of relA, Rel(Sm) , responsible for synthesis of ppGpp, shows functional similarities to E. coli SpoT. Using promoter-egfp gene fusions, we showed that in Rm2011 relA is expressed at a low rate, as a readthrough from the rpoZ promoter and from its own weak promoter. The low level of relA expression is physiologically relevant, since overexpression of Rel(Sm) inhibits ppGpp accumulation. The N-terminal portion of Rel(Sm) is required for ppGpp degradation in nutrient-sufficient cells and might be involved in regulation of the ppGpp synthase and hydrolase activities of the protein. Expression profiling of S. meliloti subjected to sudden nitrogen or carbon downshifts revealed that repression of 'house-keeping' genes is largely dependent on relA whereas activation of gene targets of the stress sigma factor RpoE2 occurred independently of relA. The regulatory genes nifA, ntrB, aniA and sinR, as well as genes related to modulation of protein biosynthesis and nucleotide catabolism, were induced in a relA-dependent manner. dksA was required for the majority of the relA-dependent regulations.
authors.affiliation: Faculty of Biology and Center for Systems Biology, University of Freiburg, Schänzlestr. 1, 79104 Freiburg, Germany.
authors.forename: Elizaveta
authors.initials: E
authors.lastname: Krol
authors.forename: Anke
authors.initials: A
authors.lastname: Becker
mesh_terms: D002244:Carbon / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D008025:Ligases / Q000096:biosynthesis* / Q000235:genetics; D009584:Nitrogen / Q000378:metabolism; D009711:Nucleotides / Q000378:metabolism; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D012808:Sigma Factor / Q000235:genetics / Q000378:metabolism; D016962:Sinorhizobium meliloti / Q000201:enzymology* / Q000235:genetics*; D013217:Starvation; D059467:Transcriptome
chemical_list: D009711:Nucleotides; D012808:Sigma Factor; D002244:Carbon; D008025:Ligases; C028812:guanosine 3',5'-polyphosphate synthetases; D009584:Nitrogen