title: Δ6-Desaturase from Mortierella alpina: cDNA cloning, expression, and phylogenetic analysis.
abstract: The open reading frame of the Δ(6)-desaturase gene was isolated from Mortierella alpina W15 and the gene was cloned into a pPIC3.5K vector. The vector was transformed into Pichia pastoris GS115 and expression was induced with methanol. The Δ(6)-desaturase expressed in P. pastoris GS115 catalyzed the conversion of linoleic acid to γ-linolenic acid but not the conversion of α-linolenic acid to octadecatetraenoic acid. The results indicate that the Δ(6)-desaturase gene from M. alpina W15 has substrate specificity in different organisms. Phylogenetic analysis revealed that Δ(6)-desaturase genes can be divided into four monophyletic groups. This work paves the way for further study of the functions of Δ(6)-desaturase in fatty acid metabolism and its three-dimensional structure.
authors.affiliation: College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Liu
authors.forename: Dianrong
authors.initials: D
authors.lastname: Li
authors.forename: Yongtai
authors.initials: Y
authors.lastname: Yin
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.forename: Maoteng
authors.initials: M
authors.lastname: Li
authors.forename: Longjiang
authors.initials: L
authors.lastname: Yu
mesh_terms: D003001:Cloning, Molecular / Q000379:methods*; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D050602:Linoleoyl-CoA Desaturase / Q000096:biosynthesis* / Q000235:genetics*; D020094:Mortierella / Q000201:enzymology* / Q000235:genetics; D010802:Phylogeny; D010843:Pichia / Q000235:genetics; D012331:RNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017965:gamma-Linolenic Acid / Q000032:analysis / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D005227:Fatty Acids; D012331:RNA, Fungal; D012333:RNA, Messenger; D011994:Recombinant Proteins; D017965:gamma-Linolenic Acid; D050602:Linoleoyl-CoA Desaturase