title: Expression of myeloperoxidase in swine influenza virus (SIV)-infected neutrophils in lungs from pigs experimentally infected with SIV subtype H1N2.
abstract: The expression of myeloperoxidase (MPO) was examined in the swine influenza virus (SIV)-infected neutrophils in the lungs of pigs experimentally infected with swine influenza virus (SIV) subtype H1N2 by immunohistochemistry. Five pigs each from the infected and non-infected group were euthanized 1, 3, 5, 7, and 10 days post-inoculation (dpi). Immunohistochemical reactivity was mainly seen in neutrophils. The score for pulmonary histopathological lesions correlated with the score for MPO immunohistochemical reactivity (r ( s ) = 0.962, P < 0.01). In addition, the score for in situ hybridization of SIV nucleic acid correlated with the score for MPO immunohistochemical reactivity (r ( s ) = 0.976, P < 0.01). These results suggest neutrophils are one of the primary effector cells in the early phase of SIV infection in pigs.
authors.affiliation: Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, 599 Gwanak-ro, Gwanak-Gu, 151-742, Seoul, Republic of Korea.
authors.forename: Bongtae
authors.initials: B
authors.lastname: Kim
authors.forename: Jeoung Hwa
authors.initials: JH
authors.lastname: Shin
authors.forename: Kiwon
authors.initials: K
authors.lastname: Han
authors.forename: Hwi Won
authors.initials: HW
authors.lastname: Seo
authors.forename: Yeonsu
authors.initials: Y
authors.lastname: Oh
authors.forename: Ikjae
authors.initials: I
authors.lastname: Kang
authors.forename: Changhoon
authors.initials: C
authors.lastname: Park
authors.forename: Bog-Hieu
authors.initials: BH
authors.lastname: Lee
authors.forename: Jin Sil
authors.initials: JS
authors.lastname: Jang
authors.forename: Sung-Hoon
authors.initials: SH
authors.lastname: Kim
authors.forename: Chanhee
authors.initials: C
authors.lastname: Chae
mesh_terms: D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005786:Gene Expression Regulation; D007150:Immunohistochemistry / Q000662:veterinary; D057221:Influenza A Virus, H1N2 Subtype / Q000276:immunology; D008168:Lung / Q000378:metabolism / Q000473:pathology* / Q000821:virology; D008171:Lung Diseases / Q000276:immunology / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D009504:Neutrophils / Q000378:metabolism* / Q000821:virology; D009976:Orthomyxoviridae Infections / Q000378:metabolism / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D009195:Peroxidase / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011897:Random Allocation; D034421:Sus scrofa; D013552:Swine; D013553:Swine Diseases / Q000378:metabolism / Q000473:pathology / Q000821:virology*
chemical_list: D009195:Peroxidase