title: Peroxisome proliferator receptor (PPAR) β/δ in psoriatic patients before and after two conventional therapeutic modalities: methotrexate and PUVA.
abstract: Peroxisome proliferator-activated receptor β/δ is a member of the nuclear hormone receptor superfamily suggested to contribute to psoriasis pathogenesis. Methotrexate and PUVA mainly target the T cell-mediated immunopathology of psoriasis. Our work aimed at estimating PPARβ/δ in psoriatic patients and investigating whether the standard therapeutic modalities (methotrexate and PUVA) exert their anti-psoriatic activity partially through altering PPARβ/δ levels. RT-PCR was used to measure PPAR β/δ mRNA levels in twenty four chronic plaque psoriasis patients. Patients were divided into two groups (12 patients each); group A received intramuscular methotrexate and group B was treated by PUVA 3 times/week in a PUVA 1000 cabin for ten weeks each, followed by measurement of PPAR β/δ mRNA levels. Twelve healthy volunteers served as controls. PPAR β/δ mRNA levels were significantly elevated in all patients and significantly decreased ten weeks after treatment, however, post treatment levels were still significantly elevated in comparison with those of controls. PPAR β/δ mRNA levels showed a significant positive correlation with disease duration.
authors.affiliation: Department of Dermatology, Cairo University, Cairo, Egypt. nermineleishi@gmail.com
authors.forename: Nermine
authors.initials: N
authors.lastname: El Eishi
authors.forename: Rehab
authors.initials: R
authors.lastname: Hegazy
authors.forename: Ola
authors.initials: O
authors.lastname: Abou Zeid
authors.forename: Olfat
authors.initials: O
authors.lastname: Shaker
mesh_terms: D000293:Adolescent; D000328:Adult; D018501:Antirheumatic Agents / Q000494:pharmacology / Q000627:therapeutic use; D005260:Female; D006801:Humans; D008297:Male; D008727:Methotrexate / Q000494:pharmacology / Q000627:therapeutic use*; D008875:Middle Aged; D047494:PPAR delta / Q000097:blood*; D048068:PPAR-beta / Q000097:blood*; D011701:PUVA Therapy; D011446:Prospective Studies; D011565:Psoriasis / Q000097:blood* / Q000188:drug therapy* / Q000276:immunology; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055815:Young Adult
chemical_list: D018501:Antirheumatic Agents; D047494:PPAR delta; D048068:PPAR-beta; D012333:RNA, Messenger; D008727:Methotrexate