title: [Study on discriminating nephrotoxic components in Zexie].
abstract: OBJECTIVE
  To sift and identify the nephrotoxic components in Zexie for controlling the quality of the herb.
  
  
  METHODS
  The fractions of zexie were prepared by Pre-HPLC, then the nephrotoxicity of the fraction was sifted using LLC-PK1 labelled with fluorescein diacetate and MTT assay. Finally, the compounds in the most obvious nephrotoxic fraction were identified with LC-MS.
  
  
  RESULTS
  Using MTT and FDA assay, similar results were obtained. Fraction C13 was found to be the most toxic with FDA assay, in which three compounds, alisol C, 16, 23-oxido-alisol B and alisol O, were detected and characterized by multi -stage mass spectrometric analysis.
  
  
  CONCLUSIONS
  Alisol C, 16, 23-oxido-alisol B and alisol O in Zexie may cause nephrotoxicity.
authors.affiliation: Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China. zhaoxiaoping8686@sina.com
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Zhao
authors.forename: Lin
authors.initials: L
authors.lastname: Lu
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Zhang
authors.forename: Boli
authors.initials: B
authors.lastname: Zhang
mesh_terms: D034902:Alisma / Q000737:chemistry / Q000633:toxicity*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D007668:Kidney / Q000187:drug effects*; D018374:LLC-PK1 Cells; D013058:Mass Spectrometry; D013552:Swine