title: Chemical activation of MEK1--a redox trigger for evaluating the effects of phosphorylation.
abstract: An approach to generate mimics of phosphorylated serine proteins chemically through site-specific sulfonation of cysteine is presented. This chemical modification is reversible in the presence of reducing agent and therefore is analogous to the kinase/phosphatase system used in nature.
authors.affiliation: Department of Chemistry, Tufts University, Medford, Massachusetts 02155, USA.
authors.forename: Toni L
authors.initials: TL
authors.lastname: Lamoureaux
authors.forename: David H
authors.initials: DH
authors.lastname: Lee
mesh_terms: D000906:Antibodies / Q000276:immunology; D003545:Cysteine / Q000737:chemistry; D004789:Enzyme Activation; D048369:MAP Kinase Kinase 1 / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D010084:Oxidation-Reduction; D010766:Phosphorylation; D019163:Reducing Agents / Q000737:chemistry; D013431:Sulfates / Q000737:chemistry
chemical_list: D000906:Antibodies; D019163:Reducing Agents; D013431:Sulfates; C012036:sodium sulfate; D048369:MAP Kinase Kinase 1; D003545:Cysteine