title: Diverse macroporous spheres synthesized by multiple emulsion polymerization for protein analyses.
abstract: Hydrophilic and hydrophobic spheres (1-2 mm) with macropores (50 μm) were synthesized by SDS stabilized O/W/O and W/O/W emulsion polymerization, respectively. The hydrophilic spheres were functionalized with trypsin for protein digestion and on-line integrated with the hydrophobic spheres for the subsequent sample clean-up and enrichment with almost no back pressure.
authors.affiliation: Department of Chemistry, National Cheng Kung University, No. 1 College Road, Tainan, Taiwan.
authors.forename: Shih-Shin
authors.initials: SS
authors.lastname: Liang
authors.forename: Shun-Li
authors.initials: SL
authors.lastname: Chen
authors.forename: Shu-Hui
authors.initials: SH
authors.lastname: Chen
mesh_terms: D004655:Emulsions / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D058105:Polymerization; D011109:Polymethacrylic Acids / Q000737:chemistry; D016062:Porosity; D011506:Proteins / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D014357:Trypsin / Q000378:metabolism
chemical_list: D004655:Emulsions; D011109:Polymethacrylic Acids; D011506:Proteins; C030613:polymethacrylic acid; D012967:Sodium Dodecyl Sulfate; D014357:Trypsin