title: Identification of Mycoplasmas using a fluorophore-free microarray and infrared chemical imaging (IRCI).
abstract: A novel application of mid-infrared chemical imaging (IRCI) for the fluorophore-free detection and identification of mycoplasma species is reported for the first time. The PCR-amplified biotinylated targets hybridized to microarray probes were treated with streptavidin-gold nanoparticles followed by silver enhancement. This modification has the potential to expand the implementation of DNA microarray techniques in laboratories involved in the detection of cell substrates, other biological products, and clinical materials for the presence of mycoplasmas.
authors.affiliation: Food and Drug Administration, Center for Food Safety and Applied Nutrition, 5100 Paint Branch Parkway, College Park, MD 20740-3835, USA. magdi.mossoba@fda.hhs.gov
authors.forename: M M
authors.initials: MM
authors.lastname: Mossoba
authors.forename: V
authors.initials: V
authors.lastname: Chizhikov
authors.forename: D V
authors.initials: DV
authors.lastname: Volokhov
authors.forename: K
authors.initials: K
authors.lastname: Martinez-Diaz Milians
authors.forename: B
authors.initials: B
authors.lastname: Schoen
authors.forename: S F
authors.initials: SF
authors.lastname: Al-Khaldi
mesh_terms: D004269:DNA, Bacterial / Q000032:analysis / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D007259:Infrared Rays; D053768:Metal Nanoparticles / Q000737:chemistry; D009174:Mycoplasma / Q000235:genetics / Q000302:isolation & purification*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012834:Silver / Q000737:chemistry; D013055:Spectrophotometry, Infrared; D019809:Streptavidin / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D012834:Silver; D006046:Gold; D019809:Streptavidin