title: High fidelity neuronal networks formed by plasma masking with a bilayer membrane: analysis of neurodegenerative and neuroprotective processes.
abstract: Spatially defined neuronal networks have great potential to be used in a wide spectrum of neurobiology assays. We present an original technique for the precise and reproducible formation of neuronal networks. A PDMS membrane comprising through-holes aligned with interconnecting microchannels was used during oxygen plasma etching to dry mask a protein rejecting poly(ethylene glycol) (PEG) adlayer. Patterns were faithfully replicated to produce an oxidized interconnected array pattern which supported protein adsorption. Differentiated human SH-SY5Y neuron-like cells adhered to the array nodes with the micron-scale interconnecting tracks guiding neurite outgrowth to produce neuronal connections and establish a network. A 2.0 μm track width was optimal for high-level network formation and node compliance. These spatially standardized neuronal networks were used to analyse the dynamics of acrylamide-induced neurite degeneration and the protective effects of co-treatment with calpeptin or brain derived neurotrophic factor (BDNF).
authors.affiliation: Leibniz Institut für Analytische Wissenschaften-ISAS-e.V., Otto-Hahn-Str. 6b, 44227 Dortmund, Germany.
authors.forename: Heike
authors.initials: H
authors.lastname: Hardelauf
authors.forename: Julia
authors.initials: J
authors.lastname: Sisnaiske
authors.forename: Amir Ali
authors.initials: AA
authors.lastname: Taghipour-Anvari
authors.forename: Peter
authors.initials: P
authors.lastname: Jacob
authors.forename: Evelyn
authors.initials: E
authors.lastname: Drabiniok
authors.forename: Ulrich
authors.initials: U
authors.lastname: Marggraf
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Frimat
authors.forename: Jan G
authors.initials: JG
authors.lastname: Hengstler
authors.forename: Andreas
authors.initials: A
authors.lastname: Neyer
authors.forename: Christoph
authors.initials: C
authors.lastname: van Thriel
authors.forename: Jonathan
authors.initials: J
authors.lastname: West
mesh_terms: D020106:Acrylamide / Q000494:pharmacology; D000327:Adsorption; D019208:Brain-Derived Neurotrophic Factor / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D004151:Dipeptides / Q000494:pharmacology; D006801:Humans; D008051:Lipid Bilayers; D008567:Membranes, Artificial; D009410:Nerve Degeneration / Q000139:chemically induced; D009415:Nerve Net / Q000187:drug effects; D018696:Neuroprotective Agents / Q000494:pharmacology*; D013499:Surface Properties
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D004129:Dimethylpolysiloxanes; D004151:Dipeptides; D008051:Lipid Bilayers; D008567:Membranes, Artificial; D018696:Neuroprotective Agents; C071482:calpeptin; D020106:Acrylamide