title: Adipocytes as a new source of catecholamine production.
abstract: Catecholamines are an important regulator of lipolysis in adipose tissue. Here we show that rat adipocytes, isolated from mesenteric adipose tissue, express genes of catecholamine biosynthetic enzymes and produce catecholamines de novo. Administration of tyrosine hydroxylase inhibitor, alpha-methyl-p-tyrosine, in vitro significantly reduced concentration of catecholamines in isolated adipocytes. We hypothesize that the sympathetic innervation of adipose tissues is not the only source of catecholamines, since adipocytes also have the capacity to produce both norepinephrine and epinephrine.
authors.affiliation: Laboratory of Stress Research, Institute of Experimental Endocrinology, Slovak Academy of Sciences, Bratislava, Slovakia. peter.vargovic@gmail.com
authors.forename: Peter
authors.initials: P
authors.lastname: Vargovic
authors.forename: Jozef
authors.initials: J
authors.lastname: Ukropec
authors.forename: Marcela
authors.initials: M
authors.lastname: Laukova
authors.forename: Susannah
authors.initials: S
authors.lastname: Cleary
authors.forename: Bernhard
authors.initials: B
authors.lastname: Manz
authors.forename: Karel
authors.initials: K
authors.lastname: Pacak
authors.forename: Richard
authors.initials: R
authors.lastname: Kvetnansky
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000187:drug effects / Q000201:enzymology / Q000502:physiology*; D002001:Adipose Tissue, Brown / Q000166:cytology / Q000187:drug effects / Q000201:enzymology / Q000502:physiology; D000818:Animals; D001142:Aromatic-L-Amino-Acid Decarboxylases / Q000235:genetics / Q000378:metabolism; D002395:Catecholamines / Q000378:metabolism*; D004299:Dopamine beta-Hydroxylase / Q000235:genetics / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004837:Epinephrine / Q000378:metabolism; D006801:Humans; D008297:Male; D008643:Mesentery / Q000033:anatomy & histology; D009638:Norepinephrine / Q000378:metabolism; D010625:Phenylethanolamine N-Methyltransferase / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014446:Tyrosine 3-Monooxygenase / Q000037:antagonists & inhibitors / Q000235:genetics; D019805:alpha-Methyltyrosine / Q000494:pharmacology
chemical_list: D002395:Catecholamines; D004791:Enzyme Inhibitors; D019805:alpha-Methyltyrosine; D014446:Tyrosine 3-Monooxygenase; D004299:Dopamine beta-Hydroxylase; D010625:Phenylethanolamine N-Methyltransferase; D001142:Aromatic-L-Amino-Acid Decarboxylases; D009638:Norepinephrine; D004837:Epinephrine