title: Concentration and diversity of noroviruses detected in Luxembourg wastewaters in 2008-2009.
abstract: Noroviruses (NoV) in 78 wastewater samples from Luxembourg were quantified, cloned, and sequenced in 2008-2009. The concentrations of NoV genogroup II and the relative occurrences of certain genotypes changed significantly during the winter season. NoV genogroup I was frequently detected by real-time reverse transcription-PCR (RT-PCR), albeit at 30-fold lower concentrations than for genogroup II, hampering attempts to assess overall genetic diversity by the cloning/sequencing approach.
authors.affiliation: Dept. of Environment and Agro-biotechnologies (EVA), Centre de Recherche Public- Gabriel Lippman, 41 Rue du Brill, L-4422 Belvaux, Luxembourg.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Skraber
authors.forename: Jérémie
authors.initials: J
authors.lastname: Langlet
authors.forename: Jacques R
authors.initials: JR
authors.lastname: Kremer
authors.forename: Joël
authors.initials: J
authors.lastname: Mossong
authors.forename: Sébastien
authors.initials: S
authors.lastname: De Landtsheer
authors.forename: Jos
authors.initials: J
authors.lastname: Even
authors.forename: Claude P
authors.initials: CP
authors.lastname: Muller
authors.forename: Lucien
authors.initials: L
authors.lastname: Hoffmann
authors.forename: Henry-Michel
authors.initials: HM
authors.lastname: Cauchie
mesh_terms: D001483:Base Sequence; D017250:Caliciviridae Infections / Q000235:genetics / Q000821:virology; D005838:Genotype; D008189:Luxembourg; D008969:Molecular Sequence Data; D029322:Norovirus / Q000235:genetics / Q000302:isolation & purification*; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017423:Sequence Analysis, RNA; D012722:Sewage / Q000821:virology*
chemical_list: D012367:RNA, Viral; D012722:Sewage