title: Targeted genome editing across species using ZFNs and TALENs.
abstract: Evolutionary studies necessary to dissect diverse biological processes have been limited by the lack of reverse genetic approaches in most organisms with sequenced genomes. We established a broadly applicable strategy using zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) for targeted disruption of endogenous genes and cis-acting regulatory elements in diverged nematode species.
authors.affiliation: Howard Hughes Medical Institute (HHMI), Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720-3204, USA.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Wood
authors.forename: Te-Wen
authors.initials: TW
authors.lastname: Lo
authors.forename: Bryan
authors.initials: B
authors.lastname: Zeitler
authors.forename: Catherine S
authors.initials: CS
authors.lastname: Pickle
authors.forename: Edward J
authors.initials: EJ
authors.lastname: Ralston
authors.forename: Andrew H
authors.initials: AH
authors.lastname: Lee
authors.forename: Rainier
authors.initials: R
authors.lastname: Amora
authors.forename: Jeffrey C
authors.initials: JC
authors.lastname: Miller
authors.forename: Elo
authors.initials: E
authors.lastname: Leung
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Meng
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Edward J
authors.initials: EJ
authors.lastname: Rebar
authors.forename: Philip D
authors.initials: PD
authors.lastname: Gregory
authors.forename: Fyodor D
authors.initials: FD
authors.lastname: Urnov
authors.forename: Barbara J
authors.initials: BJ
authors.lastname: Meyer
mesh_terms: D000818:Animals; D002107:Caenorhabditis / Q000235:genetics*; D017173:Caenorhabditis elegans / Q000235:genetics*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics / Q000378:metabolism*; D018390:Gene Targeting; D017238:Genes, Helminth; D005821:Genetic Techniques; D049751:Genome, Helminth; D054643:INDEL Mutation; D016296:Mutagenesis; D050436:Regulatory Elements, Transcriptional / Q000235:genetics*; D014157:Transcription Factors / Q000737:chemistry; D019076:Transgenes; D016335:Zinc Fingers
chemical_list: D014157:Transcription Factors; C035042:endodeoxyribonuclease FokI; D015252:Deoxyribonucleases, Type II Site-Specific