title: [Simultaneous determination of five constituents in Scrophularia ningpoensis by HPLC].
abstract: OBJECTIVE
  To develop an HPLC method for the simultaneous quantitation of five constituents in Scrophularia ningpoensis.
  
  
  METHODS
  Samples were analyzed on an Agilent SB-C18 column(4.6 mm x 250 mm, 5 microm) eluted with acetonitrile and water containing 0.03% phosphate acid as mobile phases in a linear gradient mode. The flow rate was kept at 1.0 mL x min(-1), and the column temperature was set to 30 degrees C. The DAD detector wavelengths were 210, 280, 330 nm.
  
  
  RESULTS
  The linear ranges were 50-400 mg x L(-1) for harpagide, 1-40 mg x L(-1) for harpagoside, 1-20 mg x L(-1) for cinnamic acid, 0.5-4.5 mg x L(-1) for acteoside,1-60 mg x L(-1) for angoroside C, respectively. The average recoveries of the five constituents were 100.8% (RSD 0.62%), 101.7% (RSD 0.32%), 98.8% (RSD 0.48%), 99.9% (RSD 1.4%), 99.2% (RSD 1.1%), respectively.
  
  
  CONCLUSIONS
  Through the validation, the method was proved to be sensitive, accurate, repeatable, and can be used for quality control of the roots of S. ningpoensis.
authors.affiliation: Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors.forename: Xuemei
authors.initials: X
authors.lastname: Zhang
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.forename: Rui
authors.initials: R
authors.lastname: An
authors.forename: Ximin
authors.initials: X
authors.lastname: Wu
authors.forename: Xinhong
authors.initials: X
authors.lastname: Wang
authors.forename: Yiming
authors.initials: Y
authors.lastname: Li
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002934:Cinnamates / Q000032:analysis; D003373:Coumaric Acids / Q000032:analysis; D005960:Glucosides / Q000032:analysis; D006027:Glycosides / Q000032:analysis; D057889:Iridoid Glycosides; D010636:Phenols / Q000032:analysis; D011714:Pyrans / Q000032:analysis; D032266:Scrophularia / Q000737:chemistry*; D014312:Trisaccharides / Q000032:analysis; D059808:Polyphenols
chemical_list: D002934:Cinnamates; D003373:Coumaric Acids; D005960:Glucosides; D006027:Glycosides; D057889:Iridoid Glycosides; D010636:Phenols; D011714:Pyrans; D014312:Trisaccharides; C413578:angoroside C; C029010:cinnamic acid; C058956:acteoside; D059808:Polyphenols; C033249:harpagoside; C033250:harpagide