title: Differential gene expression in pepper (Capsicum annuum) exposed to UV-B.
abstract: In the present paper, complementary DNA-amplified fragment length polymorphism (cDNA-AFLP) was used to examine and identify differentially expressed genes in Capsicum annuum exposed to UV-B irradiation. Around 4000 transcript derived fragments (TDFs) were visualized and in total 183 TDFs were isolated, sequenced and analyzed by Blast 2 go. Among these TDFs, 84 of them showed homology to known genes. There were 43 TDFs showing up-regulated expression, 24 TDFs showing down-regulated expression and 29 TDFs showing both up-regulated and down-regulated expression, respectively. Some of these TDFs were found to be in response/related to UV-B stress, including carbonic anhydrase, calcium-dependent protein, thionin-like protein, bzip protein and so on. In particular, chlorophyll a/b binding protein (Capcab) responding to UV-B stress was cloned. It was concluded that Capcab could play a protective role in plant anti-UV-B and maintaining photosynthetic rate under UV-B stress.
authors.affiliation: School of Life Sciences, Fujian Agriculture and Forestry University, 350002 Fuzhou, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Lai
authors.forename: Bo
authors.initials: B
authors.lastname: Xu
authors.forename: Li
authors.initials: L
authors.lastname: He
authors.forename: Ming
authors.initials: M
authors.lastname: Lin
authors.forename: Lei
authors.initials: L
authors.lastname: Cao
authors.forename: Shaoliang
authors.initials: S
authors.lastname: Mou
authors.forename: Yang
authors.initials: Y
authors.lastname: Wu
authors.forename: Shuilin
authors.initials: S
authors.lastname: He
mesh_terms: D001483:Base Sequence; D002212:Capsicum / Q000235:genetics* / Q000528:radiation effects*; D018744:DNA, Plant / Q000235:genetics; D015870:Gene Expression / Q000528:radiation effects; D017343:Genes, Plant / Q000528:radiation effects; D010940:Plant Proteins / Q000235:genetics; D011836:Radiation Tolerance / Q000235:genetics; D013312:Stress, Physiological / Q000235:genetics / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D018744:DNA, Plant; D010940:Plant Proteins