title: Modified Giemsa method for confirmation of Pneumocystis pneumonia in low-income countries.
abstract: An HIV-seropositive man who presented with cough, low-grade fever and difficulty in breathing was admitted to the pulmonology unit at Mulago Hospital in Kampala, Uganda. He was initially diagnosed with bacterial pneumonia and treated with ceftriaxone without significant improvement. Induced sputum and bronchoalveolar lavage specimens were collected and stained using a modified Giemsa stain (Diff-Quik). With this technique, it was possible to demonstrate cystic and trophic forms of Pneumocystis jirovecii and confirm the diagnosis of Pneumocystis pneumonia in this patient.
authors.affiliation: Makerere University, Medicine, Mulago Hospital, New Mulago, Kampala, 7051, Uganda.
authors.forename: Alfred Onubia
authors.initials: AO
authors.lastname: Andama
authors.forename: Adithya
authors.initials: A
authors.lastname: Cattamanchi
authors.forename: J Lucian
authors.initials: JL
authors.lastname: Davis
authors.forename: Saskia
authors.initials: S
authors.lastname: den Boon
authors.forename: William
authors.initials: W
authors.lastname: Worodria
authors.forename: Laurence
authors.initials: L
authors.lastname: Huang