title: Kinetics of catalyzed hydrolysis of 4-methylumbelliferyl caprylate (MUCAP) salmonella reagent.
abstract: The kinetics of chemical hydrolysis including neutral, acid- and base-catalyzed hydrolysis of 4-methylumbelliferyl caprylate (MUCAP) salmonella reagent were studied at different temperatures. The rate constants and activation parameters were determined by following the build-up of fluorescence peak of the hydrolysis product 4-methylumbelliferone (4-MU). The time scale of esterase enzyme hydrolysis caused by salmonella was compared with chemical hydrolysis as a background process.
authors.affiliation: Chemistry Department, Faculty of Science, Tanta University, Tanta, Egypt.
authors.forename: Ahmed S
authors.initials: AS
authors.lastname: Al-Kady
authors.forename: El-Sadat I
authors.initials: el-SI
authors.lastname: Ahmed
authors.forename: M
authors.initials: M
authors.lastname: Gaber
authors.forename: Mohamed M
authors.initials: MM
authors.lastname: Hussein
authors.forename: El-Zeiny M
authors.initials: el-ZM
authors.lastname: Ebeid
mesh_terms: D015373:Bacterial Typing Techniques; D006868:Hydrolysis; D006923:Hymecromone / Q000031:analogs & derivatives* / Q000737:chemistry; D007700:Kinetics; D012475:Salmonella / Q000145:classification / Q000302:isolation & purification*; D012480:Salmonella Infections / Q000175:diagnosis*
chemical_list: C072615:4-methylumbelliferyl caprylate; D006923:Hymecromone