title: Identification of guanylate-binding protein 1 as a potential oral cancer marker involved in cell invasion using omics-based analysis.
abstract: Oral cavity squamous cell carcinoma (OSCC) is a devastating disease that accounts for 3% of all cancer cases diagnosed annually. OSCC is usually diagnosed at advanced clinical stages, resulting in poor outcomes. To identify effective biomarkers for improved OSCC diagnosis and/or management, we simultaneously analyzed the OSCC cell secretome and tissue transcriptome. Among the 19 candidates isolated, guanylate-binding protein 1 (GBP1) was selected for further validation using serum samples from OSCC patients and healthy controls. Notably, the serum level of GBP1 was higher in OSCC patients, compared to that in healthy controls. Immunohistochemical analysis further revealed GBP1 overexpression in OSCC tissues, compared with adjacent noncancerous epithelia. Importantly, the higher GBP1 level in OSCC tissue was associated with higher overall pathological stage, positive perineural invasion, and poorer prognosis. Moreover, GBP1 modulated the migration and invasion of OSCC cells in vitro. Our results collectively indicate that integrated analysis of the cancer secretome and transcriptome is a feasible strategy for the efficient identification of novel OSCC markers.
authors.affiliation: Department of Cell and Molecular Biology, Chang Gung University, Taoyuan, Taiwan.
authors.forename: Chia-Jung
authors.initials: CJ
authors.lastname: Yu
authors.forename: Kai-Ping
authors.initials: KP
authors.lastname: Chang
authors.forename: Yin-Ju
authors.initials: YJ
authors.lastname: Chang
authors.forename: Chia-Wei
authors.initials: CW
authors.lastname: Hsu
authors.forename: Ying
authors.initials: Y
authors.lastname: Liang
authors.forename: Jau-Song
authors.initials: JS
authors.lastname: Yu
authors.forename: Lang-Ming
authors.initials: LM
authors.lastname: Chi
authors.forename: Yu-Sun
authors.initials: YS
authors.lastname: Chang
authors.forename: Chih-Ching
authors.initials: CC
authors.lastname: Wu
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D016022:Case-Control Studies; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D017077:Culture Media, Conditioned; D004591:Electrophoresis, Polyacrylamide Gel; D019204:GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D009062:Mouth Neoplasms / Q000378:metabolism* / Q000473:pathology; D009361:Neoplasm Invasiveness; D034741:RNA, Small Interfering; D053719:Tandem Mass Spectrometry
chemical_list: D014408:Biomarkers, Tumor; D017077:Culture Media, Conditioned; C490287:GBP1 protein, human; D034741:RNA, Small Interfering; D019204:GTP-Binding Proteins