title: Bacterial growth rate reflects a bottleneck in resource allocation.
abstract: BACKGROUND
  Growth rate management in fast-growing bacteria is currently an active research area. In spite of the huge progress made in our understanding of the molecular mechanisms controlling the growth rate, fundamental questions concerning its intrinsic limitations are still relevant today. In parallel, systems biology claims that mathematical models could shed light on these questions.
  
  
  METHODS
  This review explores some possible reasons for the limitation of the growth rate in fast-growing bacteria, using a systems biology approach based on constraint-based modeling methods.
  
  
  RESULTS
  Recent experimental results and a new constraint-based modelling method named Resource Balance Analysis (RBA) reveal the existence of constraints on resource allocation between biological processes in bacterial cells. In this context, the distribution of a finite amount of resources between the metabolic network and the ribosomes limits the growth rate, which implies the existence of a bottleneck between these two processes. Any mechanism for saving resources increases the growth rate.
  
  
  CONCLUSIONS
  Consequently, the emergence of genetic regulation of metabolic pathways, e.g. catabolite repression, could then arise as a means to minimise the protein cost, i.e. maximising growth performance while minimising the resource allocation. This article is part of a Special Issue entitled Systems Biology of Microorganisms.
authors.affiliation: Institut National de la Recherche en Agronomie, Unité de Mathématique, Informatique et Génome, Jouy-en-Josas, France. anne.goelzer@jouy.inra.fr
authors.forename: A
authors.initials: A
authors.lastname: Goelzer
authors.forename: V
authors.initials: V
authors.lastname: Fromion
mesh_terms: D001419:Bacteria / Q000254:growth & development* / Q000378:metabolism*; D008962:Models, Theoretical; D049490:Systems Biology / Q000379:methods