title: Effects of the Topical Application of Hydroalcoholic Leaf Extract of Oncidium flexuosum Sims. (Orchidaceae) and Microcurrent on the Healing of Wounds Surgically Induced in Wistar Rats.
abstract: This study evaluated the wound healing activity of hydroalcoholic leaf extract of Oncidium flexuosum Sims. (Orchidaceae), an important native plant of Brazil, combined or not with microcurrent stimulation. Wistar rats were randomly divided into four groups of nine animals: control (C), topical application of the extract (OF), treated with a microcurrent (10 μA/2 min) (MC), and topical application of the extract plus microcurrent (OF + MC). Tissue samples were obtained 2, 6, and 10 days after injury and submitted to structural and morphometric analysis. The simultaneous application of OF + MC was found to be highly effective in terms of the parameters analyzed (P < .05), with positive effects on the area of newly formed tissue, number of fibroblasts, number of newly formed blood vessels, and epithelial thickness. Morphometric data confirmed the structural findings. The O. flexuosum leaf extract contains active compounds that speed the healing process, especially when applied simultaneously with microcurrent stimulation.
authors.affiliation: Núcleo de Ciências da Saúde do Centro Universitário Hermínio Ometto (UNIARARAS), Av. Dr. Maximiliano Baruto, 500, Araras, CEP: 13607-339, SP, Brazil.
authors.forename: Fernanda Oliveira
authors.initials: FO
authors.lastname: de G de Gaspi
authors.forename: Mary Ann
authors.initials: MA
authors.lastname: Foglio
authors.forename: João Ernesto
authors.initials: JE
authors.lastname: de Carvalho
authors.forename: Gláucia Maria T
authors.initials: GM
authors.lastname: Santos
authors.forename: Milene
authors.initials: M
authors.lastname: Testa
authors.forename: José Roberto
authors.initials: JR
authors.lastname: Passarini
authors.forename: Cristiano Pedroso
authors.initials: CP
authors.lastname: de Moraes
authors.forename: Marcelo A Marreto
authors.initials: MA
authors.lastname: Esquisatto
authors.forename: Josué S
authors.initials: JS
authors.lastname: Mendonça
authors.forename: Fernanda A Sampaio
authors.initials: FA
authors.lastname: Mendonça