title: Formulation of curcumin-loaded solid lipid nanoparticles produced by fatty acids coacervation technique.
abstract: Curcumin (CU) loaded solid lipid nanoparticles (SLNs) of fatty acids (FA) were prepared with a coacervation technique based on FA precipitation from their sodium salt micelles in the presence of polymeric non-ionic surfactants. Myristic, palmitic, stearic, and behenic acids, and different polymers with various molecular weights and hydrolysis grades were employed as lipid matrixes and stabilisers, respectively. Generally, spherical-shaped nanoparticles with mean diameters below 500 nm were obtained, and using only middle-high hydrolysis, grade-polymer SLNs with diameters lower than 300 nm were produced. CU encapsulation efficiency was in the range 28-81% and highly influenced by both FA and polymer type. Chitosan hydrochloride was added to FA SLN formulations to produce bioadhesive, positively charged nanoparticles. A CU-chitosan complex formation could be hypothesised by DSC analysis, UV-vis spectra and chitosan surface tension determination. A preliminary study on HCT-116 colon cancer cells was developed to evaluate the influence of CU-loaded FA SLNs on cell viability.
authors.affiliation: Dipartimento di Scienza e Tecnologia del Farmaco, University of Torino, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Chirio
authors.forename: Marina
authors.initials: M
authors.lastname: Gallarate
authors.forename: Elena
authors.initials: E
authors.lastname: Peira
authors.forename: Luigi
authors.initials: L
authors.lastname: Battaglia
authors.forename: Loredana
authors.initials: L
authors.lastname: Serpe
authors.forename: Michele
authors.initials: M
authors.lastname: Trotta
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D030024:Curcuma / Q000737:chemistry; D003474:Curcumin / Q000008:administration & dosage* / Q000494:pharmacology; D004337:Drug Carriers / Q000737:chemistry*; D005227:Fatty Acids / Q000737:chemistry*; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009369:Neoplasms / Q000188:drug therapy
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D005227:Fatty Acids; D003474:Curcumin