title: Mast cells are key promoters of contact allergy that mediate the adjuvant effects of haptens.
abstract: A prominent feature of sensitizing environmental compounds that cause allergic contact dermatitis is the rapid induction of an innate inflammatory response that seems to provide danger signals for efficient T cell priming. We generated mouse models of mast cell deficiency, mast cell-specific gene inactivation, and mast cell reporter mice for intravital imaging and showed that these adjuvant effects of contact allergens are mediated by mast cells and histamine. Mast cell deficiency resulted in impaired emigration of skin DCs to the lymph node and contact hypersensitivity was dramatically reduced in the absence of mast cells. In addition, mast cell-specific inactivation of the Il10 gene did not reveal any role for mast cell-derived IL-10 in the regulation of contact allergy. Collectively, we demonstrate that mast cells are essential promoters of contact hypersensitivity, thereby highlighting their potential to promote immune responses to antigens entering via the skin.
authors.affiliation: Institute for Immunology, University of Technology Dresden, Medical Faculty Carl-Gustav Carus, 01307 Dresden, Germany.
authors.forename: Anne
authors.initials: A
authors.lastname: Dudeck
authors.forename: Jan
authors.initials: J
authors.lastname: Dudeck
authors.forename: Julia
authors.initials: J
authors.lastname: Scholten
authors.forename: Anke
authors.initials: A
authors.lastname: Petzold
authors.forename: Sangeetha
authors.initials: S
authors.lastname: Surianarayanan
authors.forename: Anja
authors.initials: A
authors.lastname: Köhler
authors.forename: Katrin
authors.initials: K
authors.lastname: Peschke
authors.forename: David
authors.initials: D
authors.lastname: Vöhringer
authors.forename: Claudia
authors.initials: C
authors.lastname: Waskow
authors.forename: Thomas
authors.initials: T
authors.lastname: Krieg
authors.forename: Werner
authors.initials: W
authors.lastname: Müller
authors.forename: Ari
authors.initials: A
authors.lastname: Waisman
authors.forename: Karin
authors.initials: K
authors.lastname: Hartmann
authors.forename: Matthias
authors.initials: M
authors.lastname: Gunzer
authors.forename: Axel
authors.initials: A
authors.lastname: Roers
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D002465:Cell Movement; D003713:Dendritic Cells / Q000276:immunology; D017449:Dermatitis, Allergic Contact / Q000276:immunology*; D006241:Haptens / Q000276:immunology*; D006632:Histamine / Q000276:immunology; D006984:Hypertrophy / Q000276:immunology; D007113:Immunity, Innate; D008198:Lymph Nodes / Q000276:immunology; D008407:Mast Cells / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009154:Mutation; D009389:Neovascularization, Pathologic / Q000139:chemically induced / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D006241:Haptens; D006632:Histamine