title: Comparative antioxidant capacities of quercetin and butylated hydroxyanisole in cholesterol-modified erythrocytes damaged by tert-butylhydroperoxide.
abstract: Phenolic compounds are potent antioxidants that scavenge reactive oxygen species (ROS), protecting the cells against oxidative damage. Their antioxidant capacities are governed by their structural features and the nature and physical state of the cell membrane. Our study compares the protective effects of butylated hydroxyanisole (BHA) and quercetin against the cellular injury induced by oxidative stress, and the influence of membrane cholesterol contents in their antioxidant capacities, analyzing the structural changes and cellular stability of native and cholesterol-modified erythrocytes exposed to tert-butylhydroperoxide in presence of each antioxidant. The data provide clear evidence that BHA affords better protection than quercetin against ROS generation, lipid peroxidation and lipid and GSH losses in oxidized erythrocytes. However, cellular integrity and stability are better protected by quercetin owing to the hemolytic effect of BHA. Both antioxidants suppress the alterations in membrane fluidity with similar efficiency, reducing methemoglobin formation in all oxidized erythrocytes. Membrane cholesterol depletion decreases the protection against the oxidative damage provided by both antioxidants. This lower preservation may be due to low antioxidant contents, a lower antioxidant capacity, or even to an increased oxidative damage in this membrane type as a consequence of environment modifications after cholesterol depletion.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Universidad de Salamanca, Plaza Doctores de Reina s/n, 37007 Salamanca, Spain.
authors.forename: José I
authors.initials: JI
authors.lastname: Sánchez-Gallego
authors.forename: Abel
authors.initials: A
authors.lastname: López-Revuelta
authors.forename: Angel
authors.initials: A
authors.lastname: Hernández-Hernández
authors.forename: José L
authors.initials: JL
authors.lastname: Sardina
authors.forename: Guillermo
authors.initials: G
authors.lastname: López-Ruano
authors.forename: Jesús
authors.initials: J
authors.lastname: Sánchez-Yagüe
authors.forename: Marcial
authors.initials: M
authors.lastname: Llanillo
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002083:Butylated Hydroxyanisole / Q000494:pharmacology*; D002784:Cholesterol / Q000737:chemistry*; D004912:Erythrocytes / Q000737:chemistry / Q000187:drug effects*; D008297:Male; D011794:Quercetin / Q000494:pharmacology*; D011817:Rabbits; D020122:tert-Butylhydroperoxide / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D002083:Butylated Hydroxyanisole; D020122:tert-Butylhydroperoxide; D002784:Cholesterol; D011794:Quercetin