title: Bacterial proteome of streptococcus pneumoniae through multidimensional separations coupled with LC-MS/MS.
abstract: Streptococcus pneumoniae is a major human respiratory pathogen causing considerable morbidity and mortality worldwide. In order to better understand the pathogenesis of S. pneumoniae, we employed SDS-PAGE combined with LC-MS/MS analysis and in-solution digestion coupled with 2D-LC-MS/MS to obtain the whole-cell proteome of the bacterium. Among the identified 1,210 proteins, 345 proteins were annotated for cellular components, 613 for biological processes, and 421 for molecular functions. Important virulence-associated surface proteins such as Eno, ZmpB, and PrtA were identified. Classification analysis and protein-protein interaction map revealed that these identified proteins are involved in many biological processes including protein biosynthesis, protein folding and proteolysis, cell cycle, or regulation and carbohydrate metabolism. These data represent a comprehensive reference map of S. pneumoniae proteome, providing a useful source for further analysis of the virulence factors and the regulatory network involved in the pathogenesis of the bacterium.
authors.affiliation: Institute of Life and Health Engineering/National Engineering Research Center of Genetic Medicine, Jinan University, Guangzhou, People's Republic of China. tsunxs@jnu.edu.cn
authors.forename: Xuesong
authors.initials: X
authors.lastname: Sun
authors.forename: Hong-Ling
authors.initials: HL
authors.lastname: Jia
authors.forename: Chuan-Le
authors.initials: CL
authors.lastname: Xiao
authors.forename: Xing-Feng
authors.initials: XF
authors.lastname: Yin
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Yang
authors.forename: Jie
authors.initials: J
authors.lastname: Lu
authors.forename: Xiang
authors.initials: X
authors.lastname: He
authors.forename: Nan
authors.initials: N
authors.lastname: Li
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Qing-Yu
authors.initials: QY
authors.lastname: He
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D002853:Chromatography, Liquid / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D020543:Proteome / Q000378:metabolism*; D013296:Streptococcus pneumoniae / Q000378:metabolism*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D001426:Bacterial Proteins; D020543:Proteome