title: Antigen-specific T-regulatory cells can extend skin graft survival time in mice.
abstract: This study investigated the effects of donor antigen-specific CD4(+)CD25(+) T-regulatory cells (Tregs) on skin allografts in mice. An allogeneic skin transplant model was established using donor C57BL/6 or DBA and recipient BALB/c mice. Recipients were divided into 4 groups: control group without intervention (CON; C57BL/6 to BALB/c), rapamycin gavage group (RAP; C57BL/6 to BALB/c), CD4(+)CD25(+) Tregs-treated group (TRE; C57BL/6 to BALB/c), in which recipients received transfusions of CD4(+)CD25(+) Tregs stimulated with C57BL/6-derived immature dendritic cells, and the third-party donor group (DBA; DBA to BALB/c) in which recipients received transfusions of BALB/c CD4(+)CD25(+) Tregs stimulated with C57BL/6-derived immature dendritic cells. Mean (SD) survival time of the skin allografts in the TRE group was 17.0 (3.4) days, significantly longer than in the other groups: CON, 6.9 (1.9) days; RAP, 10.3 (3.0) days; and DBA, 10.8 (3.6) days. The TRE group demonstrated a significantly greater expression of transforming growth factor-β and interleukin (IL)-10. Donor antigen-specific CD4(+)CD25(+) Tregs effectively extend skin allograft survival in mice.
authors.affiliation: Department of Gastrointestinal surgery, First People's Hospital of Foshan City, Foshan, China.
authors.forename: S
authors.initials: S
authors.lastname: Yu
authors.forename: B
authors.initials: B
authors.lastname: Fu
authors.forename: X
authors.initials: X
authors.lastname: He
authors.forename: X
authors.initials: X
authors.lastname: Peng
authors.forename: A
authors.initials: A
authors.lastname: Hu
authors.forename: Y
authors.initials: Y
authors.lastname: Ma
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology*; D001483:Base Sequence; D049109:Cell Proliferation; D017931:DNA Primers; D006085:Graft Survival; D051379:Mice; D008815:Mice, Inbred Strains; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016038:Skin Transplantation; D050378:T-Lymphocytes, Regulatory / Q000166:cytology / Q000276:immunology*
chemical_list: D000941:Antigens; D017931:DNA Primers