title: Dietary restriction improves systemic and muscular oxidative stress in type 2 diabetic Goto-Kakizaki rats.
abstract: Type 2 diabetes is a heterogeneous metabolic disease characterized by insulin resistance and β-cell dysfunction leading to hyperglycaemia and dyslipidaemia. Dietary intervention seems to improve some of these cellular complications, namely insulin resistance. Our aim was to evaluate the effects of dietary restriction on systemic and skeletal muscle oxidative stress and insulin resistance in normal Wistar rats and Goto-Kakizaki (GK) rats, a non-obese type 2 diabetic animal model. Four-month-old normal and diabetic rats were separated in four groups. One group of each strain was maintained with ad libitum standard diet, and the other group was submitted to a dietary restriction (50% of control animals daily food intake), during 2 months. Metabolic profile, insulin resistance indexes and muscle lipids were determined. Oxidative stress parameters were also measured at systemic and muscle levels: protein carbonyl, 8-hydroxy-2'-deoxyguanosine and free 8-isoprostane. Dietary restriction improved lipid profile in both strains and urinary free 8-isoprostane and plasma carbonyl compounds in diabetic rats. An improvement of muscle triglycerides accumulation and 8-isoprostane concentration and a reduction of insulin resistance were also observed in GK rats. Our data show that dietary restriction ameliorates systemic and skeletal muscle oxidative stress state in type 2 diabetes, which is associated with improved insulin resistance.
authors.affiliation: Institute of Physiology, Faculty of Medicine of University of Coimbra, Sub-unit 1, Polo III, Azinhaga de Santa Comba, Celas, 3000-354, Coimbra, Portugal. lisa1cor@hotmail.com
authors.forename: Lisa
authors.initials: L
authors.lastname: Rodrigues
authors.forename: Joana
authors.initials: J
authors.lastname: Crisóstomo
authors.forename: Paulo
authors.initials: P
authors.lastname: Matafome
authors.forename: Teresa
authors.initials: T
authors.lastname: Louro
authors.forename: Elsa
authors.initials: E
authors.lastname: Nunes
authors.forename: Raquel
authors.initials: R
authors.lastname: Seiça
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000652:urine; D003924:Diabetes Mellitus, Type 2 / Q000178:diet therapy* / Q000378:metabolism; D003927:Diet, Diabetic; D004038:Diet, Reducing; D015237:Dinoprost / Q000031:analogs & derivatives / Q000652:urine; D004435:Eating; D007333:Insulin Resistance; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism*; D018384:Oxidative Stress / Q000502:physiology; D050050:Protein Carbonylation; D051381:Rats; D020334:Rats, Inbred OLETF; D017208:Rats, Wistar; D014280:Triglycerides / Q000378:metabolism*
chemical_list: D001786:Blood Glucose; D014280:Triglycerides; C075750:8-epi-prostaglandin F2alpha; D000080242:8-Hydroxy-2'-Deoxyguanosine; D002784:Cholesterol; D015237:Dinoprost; D003849:Deoxyguanosine