title: SNPman: a program for genotype calling using run data from TaqMan allelic discrimination.
abstract: CONCLUSIONS
  The SNPman program calls the genotypes of single nucleotide polymorphisms (SNP) from TaqMan allelic discrimination assays. It utilizes the fluorescence data collected over the whole PCR run, rather than relying on the end point fluorescence measurements that is the basis of the genotype calling process in most software solutions sold with the real-time instruments. This inspection of run data facilitates genotype calls in difficult sample sets, especially in those containing various concentrations of DNA or inhibitors, as indicated by results of a reanalysis of 3738 genotyping samples. The program works with data from three different widely used PCR instruments.
  
  
  BACKGROUND
  The compiled program is available online at http://sourceforge.net/projects/snpman/files/, along with its user documentation and demonstration data files. It is free of charge for non-commercial users.
  
  
  BACKGROUND
  Ondrej.Cinek@Lfmotol.cuni.cz
  
  
  BACKGROUND
  Supplementary data are available at Bioinformatics online.
authors.affiliation: Faculty of Biomedical Engineering, Czech Technical University in Prague, Kladno, The Czech Republic.
authors.forename: Martin
authors.initials: M
authors.lastname: Konopac
authors.forename: Petra
authors.initials: P
authors.lastname: Dusatkova
authors.forename: Ondrej
authors.initials: O
authors.lastname: Cinek
mesh_terms: D000483:Alleles; D060005:Genotyping Techniques; D016133:Polymerase Chain Reaction / Q000379:methods; D020641:Polymorphism, Single Nucleotide; D012984:Software