title: Over-expression of human lipoprotein lipase in mouse mammary glands leads to reduction of milk triglyceride and delayed growth of suckling pups.
abstract: BACKGROUND
  The mammary gland is a conserved site of lipoprotein lipase expression across species and lipoprotein lipase attachment to the luminal surface of mammary gland vascular endothelial cells has been implicated in the direction of circulating triglycerides into milk synthesis during lactation.
  
  
  RESULTS
  Here we report generation of transgenic mice harboring a human lipoprotein lipase gene driven by a mammary gland-specific promoter. Lipoprotein lipase levels in transgenic milk was raised to 0.16 mg/ml, corresponding to an activity of 8772.95 mU/ml. High lipoprotein lipase activity led to a significant reduction of triglyceride concentration in milk, but other components were largely unchanged. Normal pups fed with transgenic milk showed inferior growth performances compared to those fed with normal milk.
  
  
  CONCLUSIONS
  Our study suggests a possibility to reduce the triglyceride content of cow milk using transgenic technology.
authors.affiliation: State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Wang
authors.forename: Jia
authors.initials: J
authors.lastname: Tong
authors.forename: Shuping
authors.initials: S
authors.lastname: Li
authors.forename: Ran
authors.initials: R
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.forename: Yuhui
authors.initials: Y
authors.lastname: Wang
authors.forename: Min
authors.initials: M
authors.lastname: Zheng
authors.forename: Meili
authors.initials: M
authors.lastname: Wang
authors.forename: George
authors.initials: G
authors.lastname: Liu
authors.forename: Yunping
authors.initials: Y
authors.lastname: Dai
authors.forename: Yaofeng
authors.initials: Y
authors.lastname: Zhao
authors.forename: Ning
authors.initials: N
authors.lastname: Li
mesh_terms: D000818:Animals; D000833:Animals, Suckling / Q000254:growth & development*; D004797:Enzyme-Linked Immunosorbent Assay; D005230:Fatty Acids, Nonesterified / Q000032:analysis; D005243:Feces; D005260:Female; D006801:Humans; D008071:Lipoprotein Lipase / Q000235:genetics* / Q000378:metabolism; D008321:Mammary Glands, Animal / Q000201:enzymology*; D051379:Mice; D008822:Mice, Transgenic; D008892:Milk / Q000737:chemistry*; D014280:Triglycerides / Q000032:analysis*
chemical_list: D005230:Fatty Acids, Nonesterified; D014280:Triglycerides; D008071:Lipoprotein Lipase