title: Measurement method for hydroxylated polychlorinated biphenyls in the blood of Yusho patients by liquid chromatography-electrospray tandem mass spectrometry.
abstract: Hydroxylated polychlorinated biphenyls (OH-PCBs) are formed as major metabolites of PCBs by cytochrome P450 enzyme-mediated oxidation. It has been reported that their total concentration in serum samples of Yusho patients ranged from 390 to 1300 pg/g. We developed a measurement method for OH-PCBs in blood samples by LC/MS/MS. This method is effective at determining the concentrations of PCDDs, PCDFs, Co-PCBs and OH-PCBs from the same sample without special treatment of the sample. The concentration of OH-PCBs in the blood of Yusho patients was examined using this method. The major OH-PCB metabolites were 4-OH-CB187 (54-906 pg/g-wet), 4-OH-CB146 + 3-OH-CB153 (32-527 pg/g-wet), 4-OH-CB109 (ND-229 pg/g-wet) and 4'-OH-CB172 (ND-143 pg/g-wet). The total OH-PCBs ranged from 95 to 1740 pg/g-wet.
authors.affiliation: Fukuoka Institute of Health and Environmental Sciences 39 Mukaizano, Dazaifu, Fukuoka 818-0135.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Tobiishi
authors.forename: Takashi
authors.initials: T
authors.lastname: Todaka
authors.forename: Hironori
authors.initials: H
authors.lastname: Hirakawa
authors.forename: Tsuguhide
authors.initials: T
authors.lastname: Hori
authors.forename: Jumboku
authors.initials: J
authors.lastname: Kajiwara
authors.forename: Teruaki
authors.initials: T
authors.lastname: Hirata
authors.forename: Takao
authors.initials: T
authors.lastname: Iida
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Uchi
authors.forename: Masutaka
authors.initials: M
authors.lastname: Furue
mesh_terms: D002853:Chromatography, Liquid; D005506:Food Contamination; D006801:Humans; D006900:Hydroxylation; D012275:Oryza / Q000506:poisoning*; D010938:Plant Oils / Q000506:poisoning*; D011078:Polychlorinated Biphenyls / Q000097:blood* / Q000506:poisoning*; D053719:Tandem Mass Spectrometry
chemical_list: D010938:Plant Oils; D011078:Polychlorinated Biphenyls