title: High-throughput DNA isolation method for detection of Xylella fastidiosa in plant and insect samples.
abstract: We report an inexpensive, high-throughput method for isolating DNA from insect and plant samples for the purpose of detecting Xylella fastidiosa infection. Existing methods often copurify inhibitors of DNA polymerases, limiting their usefulness for PCR-based detection assays. When compared to commercially available kits, the method provides enhanced pathogen detection at a fraction of the cost.
authors.affiliation: Texas AgriLife Research, Stephenville, TX, USA. j-brady@tamu.edu
authors.forename: Jeff A
authors.initials: JA
authors.lastname: Brady
authors.forename: Jennifer B
authors.initials: JB
authors.lastname: Faske
authors.forename: Jessica M
authors.initials: JM
authors.lastname: Castañeda-Gill
authors.forename: Jonathan L
authors.initials: JL
authors.lastname: King
authors.forename: Forrest L
authors.initials: FL
authors.lastname: Mitchell
mesh_terms: D000818:Animals; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D057166:High-Throughput Screening Assays / Q000379:methods*; D007313:Insecta / Q000382:microbiology*; D010935:Plant Diseases / Q000382:microbiology; D010944:Plants / Q000382:microbiology*; D044167:Xylella / Q000235:genetics* / Q000302:isolation & purification*
chemical_list: D004269:DNA, Bacterial