title: The purification and characterization of a novel hypersensitive-like response-inducing elicitor from Verticillium dahliae that induces resistance responses in tobacco.
abstract: PevD1, a novel protein elicitor from the pathogenic cotton verticillium wilt fungus, Verticillium dahliae, induced a hypersensitive response in tobacco plants. In this paper, the elicitor was purified and analyzed using de novo sequencing. The protein-encoding pevD1 gene consists of a 468-bp open reading frame that produces a polypeptide of 155 amino acids, with a theoretical molecular weight of 16.23 kDa. The sequence of elicitor protein PevD1 was matched to the genomic sequence (GenBank accession no. ABJE 01000445.1) of a putative protein from V. dahliae strain vdls.17, but a function had not yet been reported. The pevD1 gene was expressed in Escherichia coli, and the recombinant protein was characterized for its ability to confer systemic acquired resistance to tobacco mosaic virus (TMV). Recombinant PevD1-treated plants exhibited enhanced systemic resistance compared to control, including a significant reduction in the number and size of TMV lesions on tobacco leaves. The elicitor protein-induced hydrogen peroxide production, extracellular-medium alkalization, callose deposition, phenolics metabolism, and lignin synthesis in tobacco. Our results demonstrate that elicitor-PevD1 triggers defense responses in intact tobacco plants.
authors.affiliation: Key Laboratory for Biological Control of Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, People's Republic of China.
authors.forename: Bingnan
authors.initials: B
authors.lastname: Wang
authors.forename: Xiufen
authors.initials: X
authors.lastname: Yang
authors.forename: Hongmei
authors.initials: H
authors.lastname: Zeng
authors.forename: Hua
authors.initials: H
authors.lastname: Liu
authors.forename: Tingting
authors.initials: T
authors.lastname: Zhou
authors.forename: Beibei
authors.initials: B
authors.lastname: Tan
authors.forename: Jingjing
authors.initials: J
authors.lastname: Yuan
authors.forename: Lihua
authors.initials: L
authors.lastname: Guo
authors.forename: Dewen
authors.initials: D
authors.lastname: Qiu
mesh_terms: D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification*; D015870:Gene Expression; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016366:Open Reading Frames; D010935:Plant Diseases / Q000276:immunology; D017422:Sequence Analysis, DNA; D014026:Nicotiana / Q000276:immunology* / Q000382:microbiology; D014027:Tobacco Mosaic Virus / Q000276:immunology; D020172:Verticillium / Q000737:chemistry*
chemical_list: D004271:DNA, Fungal; D005656:Fungal Proteins