title: CHRNA3 rs1051730 genotype and short-term smoking cessation.
abstract: BACKGROUND
  The rs1051730 genetic variant within the CHRNA5-A3-B4 gene cluster is associated with heaviness of smoking and has recently been reported to be associated with likelihood of stopping smoking. We investigated the potential association of rs1051730 genotype with reduced likelihood of smoking cessation in 2 cohorts of treatment-seeking smokers in primary care in the United Kingdom.
  
  
  METHODS
  Data were drawn from 2 clinical trials on which DNA was available. One sample was a randomized placebo-controlled trial of nicotine transdermal patch and the other sample an open-label trial where all participants received nicotine transdermal patch. Smoking status was biochemically verified. Logistic regression was used to assess evidence for association in each sample, and data were combined within a meta-analysis.
  
  
  RESULTS
  There was evidence of association of rs1051730 genotype with short-term (4-week) cessation in our open-label trial sample but not our placebo-controlled trial sample. When combined in a meta-analysis, this effect remained. There was no evidence of association at later follow-up intervals. Adjustment for cigarette consumption and tobacco dependence did not alter these results substantially.
  
  
  CONCLUSIONS
  Our data, taken together with previous recent studies, provide some support for a weak association between this variant and short-term smoking cessation in treatment-seeking smokers, which does not seem to operate only among those receiving nicotine replacement therapy. Moreover, the rs1051730 variant may not merely operate as a marker for dependence or heaviness of smoking.
authors.affiliation: School of Experimental Psychology, University of Bristol, Bristol, UK. marcus.munafo@bristol.ac.uk
authors.forename: Marcus R
authors.initials: MR
authors.lastname: Munafò
authors.forename: Elaine C
authors.initials: EC
authors.lastname: Johnstone
authors.forename: Donna
authors.initials: D
authors.lastname: Walther
authors.forename: George R
authors.initials: GR
authors.lastname: Uhl
authors.forename: Michael F G
authors.initials: MF
authors.lastname: Murphy
authors.forename: Paul
authors.initials: P
authors.lastname: Aveyard
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000483:Alleles; D002248:Carbon Monoxide / Q000032:analysis; D003367:Cotinine / Q000032:analysis; D004247:DNA / Q000235:genetics; D005500:Follow-Up Studies; D005819:Genetic Markers; D005838:Genotype; D006801:Humans; D016015:Logistic Models; D008875:Middle Aged; D005810:Multigene Family; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide; D011978:Receptors, Nicotinic / Q000235:genetics*; D012463:Saliva / Q000737:chemistry; D012907:Smoking / Q000453:epidemiology / Q000235:genetics*; D020340:Tobacco Use Cessation / Q000379:methods / Q000523:psychology*; D061485:Tobacco Use Cessation Devices; D014029:Tobacco Use Disorder / Q000453:epidemiology / Q000235:genetics / Q000628:therapy; D016896:Treatment Outcome; D006113:United Kingdom / Q000453:epidemiology; D055815:Young Adult
chemical_list: D002248:Carbon Monoxide; D003367:Cotinine; D004247:DNA; D005819:Genetic Markers; D011978:Receptors, Nicotinic