title: The relationship between cellular adhesion and surface roughness in polystyrene modified by microwave plasma radiation.
abstract: BACKGROUND
  Surface modification of medical polymers can improve biocompatibility. Pure polystyrene is hydrophobic and cannot provide a suitable environment for cell cultures. The conventional method for surface modification of polystyrene is treatment with plasma. In this study, conventional polystyrene was exposed to microwave plasma treatment with oxygen and argon gases for 30, 60, and 180 seconds.
  
  
  RESULTS
  Attenuated total reflection Fourier transform infrared spectra investigations of irradiated samples indicated clearly the presence of functional groups. Atomic force microscopic images of samples irradiated with inert and active gases indicated nanometric surface topography. Samples irradiated with oxygen plasma showed more roughness (31 nm) compared with those irradiated with inert plasma (16 nm) at 180 seconds. Surface roughness increased with increasing duration of exposure, which could be due to reduction of the contact angle of samples irradiated with oxygen plasma. Contact angle analysis showed reduction in samples irradiated with inert plasma. Samples irradiated with oxygen plasma showed a lower contact angle compared with those irradiated by argon plasma.
  
  
  CONCLUSIONS
  Cellular investigations with unrestricted somatic stem cells showed better adhesion, cell growth, and proliferation for samples radiated by oxygen plasma with increasing duration of exposure than those of normal samples.
authors.affiliation: Department of Chemistry, Islamic Azad University, Tonekabon Branch, Mazandaran, Iran.
authors.forename: Esmaeil
authors.initials: E
authors.lastname: Biazar
authors.forename: Majid
authors.initials: M
authors.lastname: Heidari
authors.forename: Azadeh
authors.initials: A
authors.lastname: Asefnejad
authors.forename: Azadeh
authors.initials: A
authors.lastname: Asefnezhad
authors.forename: Naser
authors.initials: N
authors.lastname: Montazeri
mesh_terms: D001128:Argon / Q000737:chemistry; D002448:Cell Adhesion; D048430:Cell Shape / Q000187:drug effects; D005312:Fetal Blood / Q000166:cytology; D005434:Flow Cytometry; D007621:Karyotyping; D008422:Materials Testing / Q000379:methods*; D018625:Microscopy, Atomic Force; D008872:Microwaves; D010100:Oxygen / Q000737:chemistry; D058626:Plasma Gases / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties
chemical_list: D058626:Plasma Gases; D011137:Polystyrenes; D001128:Argon; D010100:Oxygen