title: T7 phage displaying latent membrane protein 1 of Epstein-Barr virus elicits humoral and cellular immune responses in rats.
abstract: The latent membrane protein 1 (LMP1) encoded by Epstein-Barr virus (EBV) has become a potential target in EBV-associated tumor prevention and treatment due to its multiple biological effects. In this study, the recombinant T7 phage displaying full-length LMP1 protein was cloned and used as an immunogen to immunize rats. Results of flow cytometry, Western blot analysis, and ELISA confirmed that both humoral and cellular immune responses were elicited in the immunized rats. Our data suggested that T7 phage was an efficient antigen carrier. The recombinant T7-LMP1 phage reconstitutes the antigenic and immunogenic properties of LMP1 and can serve as a vaccine against EBV.
authors.affiliation: Department of Pathophysiology, Three Gorges University School of Medicine, Yichang Hubel 44302, P.R. China. jianminggao@163.com
authors.forename: J
authors.initials: J
authors.lastname: Gao
authors.forename: Z
authors.initials: Z
authors.lastname: Liu
authors.forename: M
authors.initials: M
authors.lastname: Huang
authors.forename: X
authors.initials: X
authors.lastname: Li
authors.forename: Z
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000818:Animals; D017123:Bacteriophage T7 / Q000235:genetics* / Q000378:metabolism; D020031:Epstein-Barr Virus Infections / Q000276:immunology* / Q000821:virology; D005260:Female; D015870:Gene Expression; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007111:Immunity, Cellular; D056724:Immunity, Humoral; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D014763:Viral Matrix Proteins / Q000008:administration & dosage / Q000235:genetics* / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology
chemical_list: C037662:EBV-associated membrane antigen, Epstein-Barr virus; D014763:Viral Matrix Proteins; D014765:Viral Vaccines