title: Catalytic properties of the eukaryotic exosome.
abstract: The eukaryotic exosome complex is built around the backbone of a 9-subunit ring similar to phosporolytic ribonucleases such as RNase PH and polynucleotide phosphorylase (PNPase). Unlike those enzymes, the ring is devoid of any detectable catalytic activities, with the possible exception of the plant version of the complex. Instead, the essential RNA decay capability is supplied by associated hydrolytic ribonucleases belonging to the Dis3 and Rrp6 families. Dis3 proteins are endowed with two different activities: the long known processive 3'-5' exonucleolytic one and the recently discovered endonucleolytic one. Rrp6 proteins are distributive exonucleases. This chapter will review the current knowledge about the catalytic properties of theses nucleases and their interplay within the exosome holocomplex.
authors.affiliation: Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw, Warsaw, Poland.
authors.forename: Aleksander
authors.initials: A
authors.lastname: Chlebowski
authors.forename: Rafał
authors.initials: R
authors.lastname: Tomecki
authors.forename: María Eugenia
authors.initials: ME
authors.lastname: Gas López
authors.forename: Bertrand
authors.initials: B
authors.lastname: Séraphin
authors.forename: Andrzej
authors.initials: A
authors.lastname: Dziembowski
mesh_terms: D056890:Eukaryota / Q000378:metabolism; D063326:Exosome Multienzyme Ribonuclease Complex; D055354:Exosomes / Q000378:metabolism; D020871:RNA Stability
chemical_list: D063326:Exosome Multienzyme Ribonuclease Complex