title: Identification of potassium dehydroandrographolidi succinas and its major metabolites in rat urine by liquid chromatography-tandem mass spectrometry.
abstract: A rapid, sensitive and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed for identification of potassium dehydroandrographolidi succinas and its metabolites in rat urine. Five male rats were administrated a single dose (100 mg/kg) of potassium dehydroandrographolidi succinas by i.v. injection. The urine were sampled from 0 to 24 h and purified by using Oasis® HLB extraction cartridge, then the purified urine samples were separated on a reversed-phase C18 column with a linear gradient and detected by an on-line MS detector. Identification and structural elucidation of the metabolites were performed by comparing their changes in molecular mass (Δm) and MS/MS spectra with those of the parent drug. Seven metabolites and the parent drug were found in rat urine. All these metabolites were reported for the first time.
authors.affiliation: School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, China. jli@zzu.edu.cn
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.forename: Zu-Yue
authors.initials: ZY
authors.lastname: Tu
authors.forename: Jun-Yuan
authors.initials: JY
authors.lastname: Yin
authors.forename: Guo-Xia
authors.initials: GX
authors.lastname: Yue
authors.forename: Hong-Min
authors.initials: HM
authors.lastname: Liu
mesh_terms: D035685:Andrographis / Q000737:chemistry*; D000818:Animals; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000652:urine*; D004365:Drugs, Chinese Herbal; D008297:Male; D053858:Metabolic Networks and Pathways; D051381:Rats; D017208:Rats, Wistar; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004224:Diterpenes; D004365:Drugs, Chinese Herbal; C030419:andrographolide