title: Effect of leptin on differentiation of human dental stem cells.
abstract: OBJECTIVE
  Mesenchymal stem cells (MSCs) were identified in adult human periodontal ligament and dental pulp that are considered as potential stem cell sources for future clinical applications in dentistry. Leptin is known as an important regulator of mesenchymal differentiation. The objective of this study was to elucidate the role of leptin on proliferation and differentiation of dental MSCs.
  
  
  METHODS
  Enhancement of cemento/odontoblastic differentiation of dental stem cells by leptin was confirmed by alizarin red S staining and alkaline phosphatase activity staining. In contrast, leptin reduced adipogenesis in both dental pulp stem cells (DPSCs) and periodontal ligament stem cells (PDLSCs) confirmed by oil red O staining and RT-PCR. The expression of adipogenic markers, lipoprotein lipase and proliferator-activated receptor γ2 (PPARγ2), were suppressed in PDLSCs incubated on media supplemented with leptin for 2 weeks.
  
  
  RESULTS
  Leptin had a relatively stronger osteogenesis promoting effect and adipogenesis suppressing effect in PDLSCs than in DPSCs.
  
  
  CONCLUSIONS
  Collectively, leptin had a relatively stronger promoting effect on cemento/odontoblastic differentiation and a suppressing effect on adipogenesis in PDLSCs than in DPSCs. This study has provided evidence that leptin acts as an important modulator of dental MSCs differentiation.
authors.affiliation: Biotooth Engineering Lab, Department of Oral and Maxillofacial Surgery, School of Dentistry BK21, Craniomaxillofacial Life Science, Dental Research Institute, Seoul National University, Seoul, Korea.
authors.forename: S
authors.initials: S
authors.lastname: Um
authors.forename: J-R
authors.initials: JR
authors.lastname: Choi
authors.forename: J-H
authors.initials: JH
authors.lastname: Lee
authors.forename: Q
authors.initials: Q
authors.lastname: Zhang
authors.forename: Bm
authors.initials: B
authors.lastname: Seo
mesh_terms: D050156:Adipogenesis / Q000187:drug effects; D000328:Adult; D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D000880:Anthraquinones; D001391:Azo Compounds; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004396:Coloring Agents; D003739:Dental Cementum / Q000187:drug effects; D003782:Dental Pulp / Q000166:cytology* / Q000187:drug effects; D003810:Dentinogenesis / Q000187:drug effects; D004337:Drug Carriers; D006801:Humans; D006882:Hydroxyapatites; D020738:Leptin / Q000494:pharmacology*; D008071:Lipoprotein Lipase / Q000037:antagonists & inhibitors; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000187:drug effects*; D051379:Mice; D008819:Mice, Nude; D009804:Odontoblasts / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects; D047495:PPAR gamma / Q000037:antagonists & inhibitors; D010513:Periodontal Ligament / Q000166:cytology* / Q000187:drug effects; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000880:Anthraquinones; D001391:Azo Compounds; D004396:Coloring Agents; D004337:Drug Carriers; D006882:Hydroxyapatites; D020738:Leptin; D047495:PPAR gamma; C094280:hydroxyapatite-tricalciumphosphate composite; C004468:Alizarin Red S; D008071:Lipoprotein Lipase; D000469:Alkaline Phosphatase; C011049:oil red O