title: Selenium supplementation prevents lipid peroxidation caused by arduous exercise in rat brain tissue.
abstract: BACKGROUND
  The objective of the present study is to examine how selenium supplementation affects lipid peroxidation in the brain tissue of rats subjected to acute swimming exercise.
  
  
  METHODS
  The study was carried out on 32 Spraque-Dawley adult male rats, which were allocated equally to four groups, namely general control (Group 1), selenium-supplemented control (0.6 mg/kg/day sodium selenite) (Group 2), swimming control (Group 3), and selenium-supplemented swimming group (6 mg/kg/day sodium selenite) (Group 4). Selenium supplementations were carried out by intraperitoneal (i.p.) route for four weeks. At the end of supplementation, animals in groups 3 and 4 were subjected to acute swimming. Brain tissue samples were collected from decapitated animals to determine malondialdehyde (MDA) levels using a spectrophotometer according to "Schimadzu-1601, Japan" Uchiyama and Mihara and GSH levels using a spectrophotometer according to Ellmann.
  
  
  RESULTS
  The highest MDA values in the brain tissue were obtained in Group 3 (p < 0.001). MDA values in Group 4 were higher than those in Groups 1 and 2 (p < 0.001). Brain tissue MDA values in Groups 1 and 2 did not differ. Group 4 had the highest brain GSH levels (p < 0.001). Brain tissue glutathione (GSH) levels in Group 3 were higher than those in Groups 1 and 2 (p < 0.001).
  
  
  CONCLUSIONS
  Results of the study indicate that acute swimming exercise in rats increased the lipid peroxidation in the brain tissue of rats, while selenium supplementation prevented the free-radical formation by enhancing the antioxidant activity (Tab. 1, Ref. 24).
authors.affiliation: City Office of Youth Sport, Konya, Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Akil
authors.forename: M
authors.initials: M
authors.lastname: Bicer
authors.forename: E
authors.initials: E
authors.lastname: Menevse
authors.forename: A K
authors.initials: AK
authors.lastname: Baltaci
authors.forename: R
authors.initials: R
authors.lastname: Mogulkoc
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D010805:Physical Conditioning, Animal; D051381:Rats; D017207:Rats, Sprague-Dawley; D012643:Selenium / Q000494:pharmacology*; D013550:Swimming
chemical_list: D008315:Malondialdehyde; D005978:Glutathione; D012643:Selenium