title: Interaction of inorganic mercury with CoA-SH and acyl-CoAs.
abstract: Sulfur containing biomolecules are involved in complexes with mercury. CoA is an important cofactor for many enzymes involved in metabolic processes. Fatty acyl-CoA-thioesters, substrates of mitochondrial ß-oxidation, are sulfur containing compounds and potential mercury ligands. The CoA-Hg(2+) complex can be easily assessed by UV-Vis spectroscopy or indirectly by antibacterial tests that reconfirmed the protective role of CoA on E. coli. The characteristics of these complexes were determined by means of FTIR spectroscopy. The reverse phase liquid chromatography combined with electrospray ionization tandem mass spectrometry was used for detection of the side-product that resulted through the cleavage of thioesters in the presence of mercury. An unexpected result was the detection of octathioic acid as a product. Our study shows that mitochondrial β-oxidation can be affected by thioesters depletion assisted by Hg(2+). The GC-MS technique could be used to detect some possible mitochondrial injuries due to the heavy metal ions.
authors.affiliation: Department of Chemistry, Al. I. Cuza University, Ave. Carol I No. 11, Jassy, Romania. robert.gradinaru@uaic.ro
authors.forename: Robert
authors.initials: R
authors.lastname: Gradinaru
authors.forename: Alin
authors.initials: A
authors.lastname: Ionas
authors.forename: Aurel
authors.initials: A
authors.lastname: Pui
authors.forename: Gheorghita
authors.initials: G
authors.lastname: Zbancioc
authors.forename: Gabi
authors.initials: G
authors.lastname: Drochioiu
mesh_terms: D000214:Acyl Coenzyme A / Q000737:chemistry*; D003065:Coenzyme A / Q000737:chemistry*; D005227:Fatty Acids / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D008628:Mercury / Q000737:chemistry*; D008962:Models, Theoretical; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000214:Acyl Coenzyme A; D005227:Fatty Acids; D008628:Mercury; D003065:Coenzyme A