title: Molecular characterization of a catalase-negative Staphylococcus aureus subsp. aureus Strain collected from a patient with mitral valve endocarditis and pericarditis revealed a novel nonsense mutation in the katA gene.
abstract: We report a case of endocarditis and pericarditis caused by catalase-negative Staphylococcus aureus. Molecular characterization revealed a novel nonsense mutation in the katA gene, leading to a loss of 238 amino acids (47% of the wild-type catalase protein), including the heme-binding site, NADPH-binding region, and Tyr-337, essential for catalysis.
authors.affiliation: Carol Yu Centre for Infection, Research Centre of Infection and Immunology, State Key Laboratory for Emerging Infectious Diseases, Department of Microbiology, The University of Hong Kong, Hong Kong.
authors.forename: Kelvin K W
authors.initials: KK
authors.lastname: To
authors.forename: Vincent C C
authors.initials: VC
authors.lastname: Cheng
authors.forename: Jasper F W
authors.initials: JF
authors.lastname: Chan
authors.forename: Amy C Y
authors.initials: AC
authors.lastname: Wong
authors.forename: Sandy
authors.initials: S
authors.lastname: Chau
authors.forename: Flora H F
authors.initials: FH
authors.lastname: Tsang
authors.forename: Shirly O T
authors.initials: SO
authors.lastname: Curreem
authors.forename: Susanna K P
authors.initials: SK
authors.lastname: Lau
authors.forename: Kwok-Yung
authors.initials: KY
authors.lastname: Yuen
authors.forename: Patrick C Y
authors.initials: PC
authors.lastname: Woo
mesh_terms: D000293:Adolescent; D002374:Catalase / Q000032:analysis* / Q000235:genetics; D016000:Cluster Analysis; D018389:Codon, Nonsense; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004697:Endocarditis, Bacterial / Q000175:diagnosis / Q000382:microbiology* / Q000473:pathology; D006801:Humans; D008297:Male; D008943:Mitral Valve / Q000473:pathology; D008969:Molecular Sequence Data; D010493:Pericarditis / Q000175:diagnosis / Q000382:microbiology* / Q000473:pathology; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013203:Staphylococcal Infections / Q000175:diagnosis* / Q000382:microbiology / Q000473:pathology; D013211:Staphylococcus aureus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D018389:Codon, Nonsense; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D002374:Catalase