title: Study on the roles of β-catenin in hydrogen peroxide-induced senescence in human skin fibroblasts.
abstract: Oxidative stress is one of the most important causes of the cellular senescence process. Previous studies showed that β-catenin can regulate FoxO3a and this association was enhanced in cells exposed to oxidative stress. It has also been reported that β-catenin can regulate some senescence-related proteins. We propose that β-catenin may play a crucial role in senescence of normal human primary skin fibroblasts (NHSFs). Here, we explored the roles and mechanisms of β-catenin on H(2)O(2)-induced senescence in NHSFs. β-catenin expression was decreased in NHSFs after H(2)O(2) treatment. Overexpression of β-catenin in NHSFs led to a marked delay of many senescent phenotypes induced by H(2)O(2). Furthermore, overexpression of β-catenin in NHSFs can antagonise the alteration of reactive oxygen species accumulation and some senescence-related proteins expression induced by H(2)O(2) treatment. Our data demonstrated that β-catenin can protect NHSFs from H(2)O(2)-induced premature senescence by alleviating oxidative stress and regulating some senescence-related molecules.
authors.forename: Li-ming
authors.initials: LM
authors.lastname: Tian
authors.forename: Hong-fu
authors.initials: HF
authors.lastname: Xie
authors.forename: Xiao
authors.initials: X
authors.lastname: Xiao
authors.forename: Ting
authors.initials: T
authors.lastname: Yang
authors.forename: Yao-hua
authors.initials: YH
authors.lastname: Hu
authors.forename: Wei-zhen
authors.initials: WZ
authors.lastname: Wang
authors.forename: Lei-shan
authors.initials: LS
authors.lastname: Liu
authors.forename: Xiang
authors.initials: X
authors.lastname: Chen
authors.forename: Ji
authors.initials: J
authors.lastname: Li
mesh_terms: D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects* / Q000502:physiology*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D018384:Oxidative Stress; D012867:Skin / Q000187:drug effects* / Q000378:metabolism* / Q000473:pathology; D015595:Skin Aging / Q000187:drug effects / Q000473:pathology; D051176:beta Catenin / Q000378:metabolism*
chemical_list: C495270:CTNNB1 protein, human; D051176:beta Catenin; D006861:Hydrogen Peroxide