title: A cyclodextrin host-guest recognition approach to an electrochemical sensor for simultaneous quantification of serotonin and dopamine.
abstract: An electrochemical sensor for simultaneous quantification of serotonin (5-hydroxytryptamine, 5-HT) and dopamine (DA) using a β-cyclodextrin/poly(N-acetylaniline)/carbon nanotube composite modified carbon paste electrode has been developed. Synergistic effect of multi-walled carbon nanotube (MWCNT) in addition to the pre-concentrating effect of β-cyclodextrin (β-CD) as well as its different inclusion complex stability with 5-HT and DA was used to construct an electrochemical sensor for quantification of these important neurotransmitters. The overlapping anodic peaks of 5-HT and DA at 428 mV on bare electrode resolved in two well-defined voltammetric peaks at 202 and 363 mV vs. Ag/AgCl respectively. The oxidation mechanism of 5-HT and DA on the surface of the electrode was investigated by cyclic voltammetry and it was found that the electrode processes are pH dependent and electrochemical oxidation of 5-HT is totally irreversible while the electrode gave a more reversible process to DA. Under optimized conditions, linear calibration curves were obtained in the range of about 4-200 μM with a detection limits down to sub-μM levels (S/N=3) after 20-s accumulation, for both. The proposed sensor was shown to be remarkably selective for 5-HT and DA in matrices containing different species including ascorbic acid and uric acid. The suitability of the developed method was tested for the determination of 5-HT and DA in the Randox Synthetic Plasma samples and acceptable recoveries were obtained for a set of spiked samples.
authors.affiliation: Chemistry Department, College of Sciences, Shiraz University, Shiraz 7145685464, Iran. abbaspour@chem.susc.ac.ir
authors.forename: Abdolkarim
authors.initials: A
authors.lastname: Abbaspour
authors.forename: Abolhassan
authors.initials: A
authors.lastname: Noori
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002417:Cattle; D004298:Dopamine / Q000032:analysis* / Q000097:blood; D055664:Electrochemical Techniques / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D012701:Serotonin / Q000032:analysis* / Q000097:blood; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D047392:beta-Cyclodextrins; D012701:Serotonin; D004298:Dopamine