title: Biocompatible artificial DNA linker that is read through by DNA polymerases and is functional in Escherichia coli.
abstract: A triazole mimic of a DNA phosphodiester linkage has been produced by templated chemical ligation of oligonucleotides functionalized with 5'-azide and 3'-alkyne. The individual azide and alkyne oligonucleotides were synthesized by standard phosphoramidite methods and assembled using a straightforward ligation procedure. This highly efficient chemical equivalent of enzymatic DNA ligation has been used to assemble a 300-mer from three 100-mer oligonucleotides, demonstrating the total chemical synthesis of very long oligonucleotides. The base sequences of the DNA strands containing this artificial linkage were copied during PCR with high fidelity and a gene containing the triazole linker was functional in Escherichia coli.
authors.affiliation: School of Chemistry, University of Southampton, Highfield, Southampton SO17 1BJ, United Kingdom.
authors.forename: Afaf H
authors.initials: AH
authors.lastname: El-Sagheer
authors.forename: A Pia
authors.initials: AP
authors.lastname: Sanzone
authors.forename: Rachel
authors.initials: R
authors.lastname: Gao
authors.forename: Ali
authors.initials: A
authors.lastname: Tavassoli
authors.forename: Tom
authors.initials: T
authors.lastname: Brown
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000138:chemical synthesis* / Q000737:chemistry / Q000235:genetics; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D018716:Molecular Mimicry; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry / Q000235:genetics; D016133:Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D014230:Triazoles / Q000737:chemistry
chemical_list: D009838:Oligodeoxyribonucleotides; D014230:Triazoles; D004247:DNA; D004259:DNA-Directed DNA Polymerase