title: Profiling of isoflavonoids in Iris germanica rhizome extracts by microprobe NMR and HPLC-PDA-MS analysis.
abstract: Lipophilic and polar extracts of iris rhizomes (Iris germanica) were submitted to a phytochemical profiling by a combination of HPLC-PDA-MS and semi-preparative HPLC/off-line microprobe NMR measurements. A total of 20 compounds were purified in sub-milligram to milligram amounts via two successive chromatographic steps. They were identified as isoflavones, isoflavone glycosides and acetovanillone by analysis of on-line MS and PDA, and off-line NMR data. A new isoflavone glycoside, iriflogenin-4'-O-gentiobioside, was subsequently isolated at preparative scale for full chemical characterization. This study demonstrates the applicability of the HPLC/off-line microprobe NMR approach as a robust means for rapid phytochemical profiling of plant extracts.
authors.affiliation: Division of Pharmaceutical Biology, University of Basel, Switzerland.
authors.forename: Cornelia
authors.initials: C
authors.lastname: Schütz
authors.forename: Melanie
authors.initials: M
authors.lastname: Quitschau
authors.forename: Matthias
authors.initials: M
authors.lastname: Hamburger
authors.forename: Olivier
authors.initials: O
authors.lastname: Potterat
mesh_terms: D000098:Acetophenones / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification*; D031323:Iris Plant / Q000737:chemistry*; D007529:Isoflavones / Q000737:chemistry / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D010936:Plant Extracts / Q000737:chemistry*; D027343:Rhizome
chemical_list: D000098:Acetophenones; D006027:Glycosides; D007529:Isoflavones; D010936:Plant Extracts; C056165:acetovanillone