title: Microfluidic systems for live cell imaging.
abstract: Microfluidic systems provide many advantages for live cell imaging, including improved cell culture micro-environments, control of flows and dynamic exposure profiles, and compatibility with existing high resolution microscopes. Here, we will discuss our approach for design and engineering of microfluidic cell culture environments as well as interfacing with standard laboratory tools and protocols. We focus on an application specific design concept, whereby a shared fabrication process is used to deliver multiple products for different biological applications. As adoption of advanced in vitro models increases, we envision the use of microfluidic cell culture technology to become commonplace.
authors.affiliation: CellASIC Corporation, Hayward, California, USA.
authors.forename: Philip
authors.initials: P
authors.lastname: Lee
authors.forename: Terry
authors.initials: T
authors.lastname: Gaige
authors.forename: Paul
authors.initials: P
authors.lastname: Hung
mesh_terms: D000465:Algorithms; D018929:Cell Culture Techniques; D003470:Culture Media; D006801:Humans; D019044:Image Cytometry / Q000295:instrumentation / Q000379:methods*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods; D010477:Perfusion / Q000379:methods; D059010:Single-Cell Analysis / Q000295:instrumentation / Q000379:methods*; D013314:Stress, Mechanical
chemical_list: D003470:Culture Media