title: Preanalytical delay reduces sensitivity of QuantiFERON-TB gold in-tube assay for detection of latent tuberculosis infection.
abstract: The effects of incubation delays on the accuracy of the QuantiFERON-TB gold in-tube assay (QFT-GIT) were measured. Compared to immediate incubation, 6- and 12-hour delays resulted in positive-to-negative reversion rates of 19% (5/26) and 22% (5/23), respectively. These findings underscore the need for standardizing QFT-GIT preanalytical practices.
authors.affiliation: 3375 Hillview Avenue, Room 1602, Palo Alto, CA 94304, USA.
authors.forename: David
authors.initials: D
authors.lastname: Doberne
authors.forename: Rajiv L
authors.initials: RL
authors.lastname: Gaur
authors.forename: Niaz
authors.initials: N
authors.lastname: Banaei
mesh_terms: D000328:Adult; D019411:Clinical Laboratory Techniques / Q000379:methods*; D006805:Human Experimentation; D006801:Humans; D007118:Immunoassay / Q000379:methods; D055985:Latent Tuberculosis / Q000175:diagnosis*; D008297:Male; D008875:Middle Aged; D012680:Sensitivity and Specificity; D013997:Time Factors