title: Multiplex real-time PCR assay for detection of methicillin-resistant Staphylococcus aureus and associated toxin genes.
abstract: We describe a real-time PCR assay for the detection of methicillin-resistant Staphylococcus aureus and genes encoding toxic shock syndrome toxin 1 and Panton-Valentine leukocidin. Rapid screening and detection of toxins is a useful tool for surveillance studies and outbreak investigations involving large numbers of isolates.
authors.affiliation: Centers for Disease Control and Prevention, Division of Healthcare Quality Promotion, 1600 Clifton Rd., NE, MS C-16, Atlanta, GA 30333, USA.
authors.forename: G E
authors.initials: GE
authors.lastname: Fosheim
authors.forename: A C
authors.initials: AC
authors.lastname: Nicholson
authors.forename: V S
authors.initials: VS
authors.lastname: Albrecht
authors.forename: B M
authors.initials: BM
authors.lastname: Limbago
mesh_terms: D001427:Bacterial Toxins / Q000032:analysis* / Q000235:genetics; D001431:Bacteriological Techniques / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics; D004768:Enterotoxins / Q000032:analysis* / Q000235:genetics; D005098:Exotoxins / Q000032:analysis* / Q000235:genetics; D005798:Genes, Bacterial; D006801:Humans; D007956:Leukocidins / Q000032:analysis* / Q000235:genetics; D008403:Mass Screening / Q000379:methods; D055624:Methicillin-Resistant Staphylococcus aureus / Q000235:genetics / Q000302:isolation & purification*; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D013203:Staphylococcal Infections / Q000382:microbiology*; D018089:Superantigens / Q000032:analysis* / Q000235:genetics
chemical_list: D001427:Bacterial Toxins; D004269:DNA, Bacterial; D004768:Enterotoxins; D005098:Exotoxins; D007956:Leukocidins; C078284:Panton-Valentine leukocidin; D018089:Superantigens; C030107:enterotoxin F, Staphylococcal