title: Dried and hydrated X-ray scattering analysis of amyloid fibrils.
abstract: Wide angle X-ray scattering is a key technique for the analysis of amyloid fibrils that can be used to -confirm the presence of a characteristic cross-beta fibril structure and to characterise the arrangement of beta-strands and beta-sheets within this fibril core. Further structural insight can be obtained by the comparison of X-ray scattering data obtained for dried and hydrated fibril samples. We describe simple techniques for the preparation of dried and hydrated fibril samples for X-ray analysis and the subsequent analysis of X-ray scattering patterns using custom built and readily available software.
authors.affiliation: Department of Chemistry, The University of Reading, Reading, UK.
authors.forename: Sally L
authors.initials: SL
authors.lastname: Gras
authors.forename: Adam M
authors.initials: AM
authors.lastname: Squires
mesh_terms: D000682:Amyloid / Q000737:chemistry*; D003890:Desiccation / Q000295:instrumentation / Q000379:methods*; D055503:Protein Multimerization; D017433:Protein Structure, Secondary; D014961:X-Ray Diffraction / Q000379:methods*
chemical_list: D000682:Amyloid