title: In vitro activity of plant extracts and alkaloids against clinical isolates of extended-spectrum β-lactamase (ESBL)-producing strains.
abstract: The antibacterial activity of 80% ethanol extracts of 10 medicinal plants collected in Yunnan (Southwest China), was tested against clinical isolates of extended-spectrum β-lactamase (ESBL)-producing strains. Their MIC values ranged between 1.56-12.50 mg/mL. The most active plant extract was Chelidonium majus L. (MIC = 1.56 mg/mL). Two potent isoquinoline alkaloids, 8-hydroxydihydrosanguinarine and 8-hydroxydihydrochelerythrine, were identified as the major active principles through bioassay-guided fractionation and identification of the active ethyl acetate fraction from C. majus, with minimum MIC/MBC values of 15.63/62.50 mg/mL.
authors.affiliation: Research Center for Natural Medicines, Kunming General Hospital, PLA, Kunming 650032, China. zuogouiyng@263.net
authors.forename: Guo-Ying
authors.initials: GY
authors.lastname: Zuo
authors.forename: Fan-Yan
authors.initials: FY
authors.lastname: Meng
authors.forename: Jun
authors.initials: J
authors.lastname: Han
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Hao
authors.forename: Gen-Chun
authors.initials: GC
authors.lastname: Wang
authors.forename: Yun-Ling
authors.initials: YL
authors.lastname: Zhang
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D000470:Alkaloids / Q000494:pharmacology; D028281:Chelidonium / Q000187:drug effects / Q000201:enzymology; D006576:Heterocyclic Compounds, 4 or More Rings / Q000737:chemistry / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: C517197:8-hydroxydihydrochelerythrine; C517196:8-hydroxydihydrosanguinarine; D000470:Alkaloids; D006576:Heterocyclic Compounds, 4 or More Rings; D010936:Plant Extracts; D001618:beta-Lactamases