title: Association of Toll-like receptor 10 and susceptibility to Crohn's disease independent of NOD2.
abstract: Impaired innate inflammatory response has a key role in the Crohn's disease (CD) pathogenesis. The aim of this study was to investigate the possible role of the TLR10-TLR1-TLR6 gene cluster in CD susceptibility. A total of 508 CD patients (284, cohort 1 and 224, cohort 2) and 576 controls were included. TLR10-TLR1-TLR6 cluster single-nucleotide polymorphisms genotyping, NOD2 mutations and TLR10 mRNA quantification were performed using TaqMan assays. Nucleotide-binding oligomerization domain containing 2 (NOD2) and Toll-like receptor (TLR) loci interaction was analyzed by logistic regression and multifactor-dimensionality reduction (MDR). Entropy-based analysis was used to interpret combination effects. One TLR10 haplotype (TLR10(GGGG)) was found associated with CD susceptibility in both cohorts, individuals with two copies had approximately twofold more risk of CD susceptibility than individuals having no copies (odds ratio=1.89, P-value=0.0002). No differences in the mRNA levels were observed among the genotypes. The strongest model for predicting CD risk according to the MDR analysis was a two-locus model including NOD2 mutations and TLR10(GGGG) haplotype (P(c)<0.0001). The interaction gain attributed to the combination of both genes was negative (IG=-2.36%), indicating redundancy or independent effects. Our results support association of the TLR10 gene with CD susceptibility. The effect of TLR10 would be independent of NOD2, suggesting different signaling pathways for both genes.
authors.affiliation: Servicio de Inmunología, Instituto de Biomedicina, Hospital Universitario Virgen del Rocío, Sevilla, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Abad
authors.forename: M F
authors.initials: MF
authors.lastname: González-Escribano
authors.forename: L M
authors.initials: LM
authors.lastname: Diaz-Gallo
authors.forename: J M
authors.initials: JM
authors.lastname: Lucena-Soto
authors.forename: J L
authors.initials: JL
authors.lastname: Márquez
authors.forename: E
authors.initials: E
authors.lastname: Leo
authors.forename: C
authors.initials: C
authors.lastname: Crivell
authors.forename: M
authors.initials: M
authors.lastname: Gómez-García
authors.forename: J
authors.initials: J
authors.lastname: Martín
authors.forename: A
authors.initials: A
authors.lastname: Núñez-Roldán
authors.forename: J R
authors.initials: JR
authors.lastname: García-Lozano
mesh_terms: D000293:Adolescent; D000328:Adult; D000483:Alleles; D016022:Case-Control Studies; D002648:Child; D003424:Crohn Disease / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006801:Humans; D008297:Male; D008875:Middle Aged; D008957:Models, Genetic; D053473:Nod2 Signaling Adaptor Protein / Q000235:genetics*; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D051194:Toll-Like Receptor 1 / Q000235:genetics; D051201:Toll-Like Receptor 10 / Q000235:genetics*; D051216:Toll-Like Receptor 6 / Q000235:genetics; D055815:Young Adult
chemical_list: C429018:NOD2 protein, human; D053473:Nod2 Signaling Adaptor Protein; D051194:Toll-Like Receptor 1; D051201:Toll-Like Receptor 10; D051216:Toll-Like Receptor 6