title: Relationship between a novel polymorphism of the C5L2 gene and coronary artery disease.
abstract: BACKGROUND
  C5L2 has been demonstrated to be a functional receptor of acylation-stimulating protein (ASP), which is a stimulator of triglyceride synthesis or glucose transport. However, little is known about the variations in the coding region of the C5L2 gene and their association with coronary artery disease (CAD).
  
  
  RESULTS
  We identified a novel single nucleotide polymorphism (SNP), 698C>T (P233L), in exon 2 using a polymerase chain reaction direct-sequencing method. This nucleotide change causes the amino-acid order from proline to leucine at codon 233. We examined the role of this SNP for CAD using two independent case-control studies: one was in the Han population (492 CAD patients and 577 control subjects) and the other was in the Uygur population (319 CAD patients and 554 control subjects). Heterozygote carriers of the 698CT genotype were more frequent among CAD patients than among controls not only in the Han population (7.3% versus 1.7%) but also in the Uygur population (4.7% versus 1.6%). The odds ratio (OR) for carriers of the 698CT genotype for CAD was 4.484 (95% confidence interval (CI): 2.197-9.174) in the Han group and 2.989 (95% CI: 1.292-6.909) in the Uygur population. After adjustment of confounding factors such as sex, age, smoking, alcohol consumption, hypertension, diabetes, as well as serum levels of triglyceride, total cholesterol, high-density lipoprotein, the difference remained significant in the Han group (P<0.001, OR = 6.604, 95% CI: 2.776-15.711) and in the Uygur group (P = 0.047, OR = 2.602, 95% CI: 1.015-6.671).
  
  
  CONCLUSIONS
  The 698CT genotype of C5L2 may be a genetic maker of CAD in the Han and Uygur population in western China.
authors.affiliation: Department of Cardiology, First Affiliated Hospital of Xinjiang Medical University, Urumqi, People's Republic of China.
authors.forename: Ying-Ying
authors.initials: YY
authors.lastname: Zheng
authors.forename: Xiang
authors.initials: X
authors.lastname: Xie
authors.forename: Yi-Tong
authors.initials: YT
authors.lastname: Ma
authors.forename: Yi-Ning
authors.initials: YN
authors.lastname: Yang
authors.forename: Zhen-Yan
authors.initials: ZY
authors.lastname: Fu
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: Li
authors.forename: Xiang
authors.initials: X
authors.lastname: Ma
authors.forename: Bang-Dang
authors.initials: BD
authors.lastname: Chen
authors.forename: Fen
authors.initials: F
authors.lastname: Liu
mesh_terms: D001483:Base Sequence; D003324:Coronary Artery Disease / Q000235:genetics*; D017931:DNA Primers; D005006:Ethnicity; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D044087:Receptor, Anaphylatoxin C5a; D019707:Receptors, Chemokine / Q000235:genetics*
chemical_list: C417003:C5aR2 protein, human; D017931:DNA Primers; D044087:Receptor, Anaphylatoxin C5a; D019707:Receptors, Chemokine