title: Kinetic modelling and microbial community assessment of anaerobic biphasic fixed film bioreactor treating distillery spent wash.
abstract: Anaerobic digestion, microbial community structure and kinetics were studied in a biphasic continuously fed, upflow anaerobic fixed film reactor treating high strength distillery wastewater. Treatment efficiency of the bioreactor was investigated at different hydraulic retention times (HRT) and organic loading rates (OLR 5-20 kg COD m⁻³ d⁻¹). Applying the modified Stover-Kincannon model to the reactor, the maximum removal rate constant (U(max)) and saturation value constant (K(B)) were found to be 2 kg m⁻³ d⁻¹ and 1.69 kg m⁻³ d⁻¹ respectively. Bacterial community structures of acidogenic and methanogenic reactors were assessed using culture-independent analyses. Sequencing of 16S rRNA genes exhibited a total of 123 distinct operational taxonomic units (OTUs) comprising 49 from acidogenic reactor and 74 (28 of eubacteria and 46 of archaea) from methanogenic reactor. The findings reveal the role of Lactobacillus sp. (Firmicutes) as dominant acid producing organisms in acidogenic reactor and Methanoculleus sp. (Euryarchaeotes) as foremost methanogens in methanogenic reactor.
authors.affiliation: BRD School of Bioscience, Sardar Patel Maidan, Vadtal Road, Satellite Campus, PO Box 39, Sardar Patel University, Vallabh Vidyanagar 388120, Gujarat, India.
authors.forename: Bhavik K
authors.initials: BK
authors.lastname: Acharya
authors.forename: Hilor
authors.initials: H
authors.lastname: Pathak
authors.forename: Sarayu
authors.initials: S
authors.lastname: Mohana
authors.forename: Yogesh
authors.initials: Y
authors.lastname: Shouche
authors.forename: Vasdev
authors.initials: V
authors.lastname: Singh
authors.forename: Datta
authors.initials: D
authors.lastname: Madamwar
mesh_terms: D000693:Anaerobiosis; D001105:Archaea / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D001419:Bacteria / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D001673:Biodegradation, Environmental; D044822:Biodiversity; D019149:Bioreactors; D006863:Hydrogen-Ion Concentration; D007194:India; D007220:Industrial Waste; D007700:Kinetics; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014865:Waste Disposal, Fluid; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000378:metabolism; D018508:Water Purification / Q000379:methods*
chemical_list: D007220:Industrial Waste; D012336:RNA, Ribosomal, 16S; D014874:Water Pollutants, Chemical