title: Defective GATA-3 expression in Th2 LCR-deficient mice.
abstract: Th2 cell differentiation is critically influenced by transcription factor GATA-3 and by various cis-acting elements including enhancers, silencers and a locus control region (LCR) in the Th2 cytokine locus. Th2 LCR-deficient Th2 cells completely lost the expression of GATA-3 and the phosphorylation of STAT6. Histone 3 lysine 4 (H3-K4) was hypomethylated in the gata3 locus in these cells. GATA-3 and STAT6 bound several regulatory regions in the gata3 locus and transactivated the expression of the gata3 gene. These results suggest that Th2 differentiation program stimulates feed-forward regulation of gata3 gene expression.
authors.affiliation: Department of Life Science, Sogang University, Seoul 121-742, Republic of Korea.
authors.forename: Soo Seok
authors.initials: SS
authors.lastname: Hwang
authors.forename: Kiwan
authors.initials: K
authors.lastname: Kim
authors.forename: Gap Ryol
authors.initials: GR
authors.lastname: Lee
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D050990:GATA3 Transcription Factor / Q000235:genetics*; D019901:Locus Control Region / Q000235:genetics*; D051379:Mice; D008817:Mice, Mutant Strains; D010766:Phosphorylation; D050800:STAT6 Transcription Factor / Q000378:metabolism; D018418:Th2 Cells / Q000166:cytology* / Q000378:metabolism; D015533:Transcriptional Activation
chemical_list: D050990:GATA3 Transcription Factor; C494748:Gata3 protein, mouse; D050800:STAT6 Transcription Factor; C494100:Stat6 protein, mouse