title: Neuroprotective effects of 3α-acetoxyeudesma-1,4(15),11(13)-trien-12,6α-olide against dopamine-induced apoptosis in the human neuroblastoma SH-SY5Y cell line.
abstract: Dopamine (DA), as a neurotoxin, can elicit severe Parkinson's disease-like syndrome by elevating intracellular reactive oxygen species (ROS) levels and apoptotic activity. We examined the inhibitory effects of 3α-acetoxyeudesma-1,4(15),11(13)-trien-12,6α-olide (AETO), purified from the leaves of Laurus nobilis L., on DA-induced apoptosis and α-synuclein (α-syn) formation in dopaminergic SH-SY5Y cells. AETO decreased the active form of caspase-3 and the levels of p53, which were accompanied by increased levels of Bcl-2 in a dose-dependent manner. Flow cytometric and Western blot analysis showed that AETO significantly inhibited DA-induced apoptosis along with suppression of intracellular tyrosinase activity, ROS generation, quinoprotein, and α-syn formation (P < 0.01). These results indicate that AETO inhibited DA-induced apoptosis, which is closely related to the suppression of intracellular tyrosinase activity and the formation of α-syn, ROS, and quinoprotein in SH-SY5Y cells.
authors.affiliation: Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Uk
authors.initials: U
authors.lastname: Koo
authors.forename: Kung-Woo
authors.initials: KW
authors.lastname: Nam
authors.forename: Ahrom
authors.initials: A
authors.lastname: Ham
authors.forename: Dahyun
authors.initials: D
authors.lastname: Lyu
authors.forename: Bora
authors.initials: B
authors.lastname: Kim
authors.forename: Sung-Jin
authors.initials: SJ
authors.lastname: Lee
authors.forename: Kyeong Ho
authors.initials: KH
authors.lastname: Kim
authors.forename: Ki-Bong
authors.initials: KB
authors.lastname: Oh
authors.forename: Woongchon
authors.initials: W
authors.lastname: Mar
authors.forename: Jongheon
authors.initials: J
authors.lastname: Shin
mesh_terms: D000975:Antioxidants / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D004298:Dopamine / Q000494:pharmacology; D006801:Humans; D027442:Laurus / Q000737:chemistry; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors; D009447:Neuroblastoma; D018696:Neuroprotective Agents / Q000494:pharmacology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D045787:Sesquiterpenes, Eudesmane / Q000627:therapeutic use*; D051844:alpha-Synuclein / Q000096:biosynthesis
chemical_list: C569477:3-acetoxyeudesma-1,4(15),11(13)-trien-12,6-olide; D000975:Antioxidants; D018696:Neuroprotective Agents; D019253:Proto-Oncogene Proteins c-bcl-2; D017382:Reactive Oxygen Species; D045787:Sesquiterpenes, Eudesmane; D051844:alpha-Synuclein; D014442:Monophenol Monooxygenase; D053148:Caspase 3; D004298:Dopamine