title: Inducing sleep by remote control facilitates memory consolidation in Drosophila.
abstract: Sleep is believed to play an important role in memory consolidation. We induced sleep on demand by expressing the temperature-gated nonspecific cation channel Transient receptor potential cation channel (UAS-TrpA1) in neurons, including those with projections to the dorsal fan-shaped body (FB). When the temperature was raised to 31°C, flies entered a quiescent state that meets the criteria for identifying sleep. When sleep was induced for 4 hours after a massed-training protocol for courtship conditioning that is not capable of inducing long-term memory (LTM) by itself, flies develop an LTM. Activating the dorsal FB in the absence of sleep did not result in the formation of LTM after massed training.
authors.affiliation: Department of Anatomy and Neurobiology, Washington University in St. Louis, St. Louis, MO 63110, USA.
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: Donlea
authors.forename: Matthew S
authors.initials: MS
authors.lastname: Thimgan
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Laura
authors.initials: L
authors.lastname: Gottschalk
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Shaw
mesh_terms: D000818:Animals; D003213:Conditioning, Psychological; D004330:Drosophila / Q000235:genetics / Q000502:physiology*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism; D005260:Female; D057567:Memory, Long-Term / Q000502:physiology*; D023421:Models, Animal; D009043:Motor Activity; D009474:Neurons / Q000502:physiology*; D017729:Presynaptic Terminals / Q000502:physiology; D012890:Sleep / Q000502:physiology*; D012934:Social Isolation; D013696:Temperature; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D050051:Transient Receptor Potential Channels / Q000235:genetics / Q000378:metabolism
chemical_list: D029721:Drosophila Proteins; C557617:GAL4 protein, Drosophila; D014157:Transcription Factors; D050051:Transient Receptor Potential Channels