title: Bone marrow processing with the AMICUS™ separator system.
abstract: ABO incompatible bone marrow transplantation (BMT) requires processing of the donated bone marrow (BM), either erythrocyte depletion, or also a volume reduction. The AMICUS™ system was introduced in the field of peripheral blood mononuclear cell collection, showing a good performance regarding efficiency and safety. To evaluate the performance of the MNC collection program of the Amicus device for BM, we analysed our data obtained from the Amicus and the Fenwal CS3000omnix™ plus device.
  
  
  METHODS
  From 2005 to 2008, we performed 22 automated erythrocyte depletions of BM for ABO mismatched BMT in 21 patients, 11 with the Amicus (A; 10 patients) and 11 with the CS3000 (F; 11) device.
  
  
  RESULTS
  There were no statistical differences in donor age, recipient age, type of ABO mismatch, and CD34+ cell yield [group A pre 7.03 post 4.93 vs. group F pre 8.55 and post 6.2 × 10E06 cells per kilogram of bodyweight] for both devices. The efficiency for the CD34+ cell collection was lower, but not statistically significant, in the Amicus device (70% ± 12 vs. 84% ± 12; U-test P = 0.123). The erythrocyte volume in the final product was higher but not statistically significant different in the Amicus device (9.46 ± 2.3 vs. 6.98 ± 3.3 ml; U-test P = 0.17). During the evaluation period, no technical problems were observed. All patients but one, who died at d + 11, showed a sustained engraftment.
  
  
  CONCLUSIONS
  We conclude that, in principle, the Amicus device can be used for MNC collection from BM to deplete erythrocytes from BM grafts in allogeneic stem cell transplantations.
authors.affiliation: St. Anna Kinderspital, Vienna, Austria. volker.witt@stanna.at
authors.forename: V
authors.initials: V
authors.lastname: Witt
authors.forename: E
authors.initials: E
authors.lastname: Beiglböck
authors.forename: G
authors.initials: G
authors.lastname: Fritsch
mesh_terms: D000017:ABO Blood-Group System; D000293:Adolescent; D057205:Automation, Laboratory / Q000295:instrumentation; D001781:Blood Component Removal / Q000295:instrumentation*; D001788:Blood Grouping and Crossmatching; D001854:Bone Marrow Cells / Q000166:cytology*; D016026:Bone Marrow Transplantation; D002648:Child; D002675:Child, Preschool; D004912:Erythrocytes / Q000166:cytology; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D007223:Infant; D008297:Male
chemical_list: D000017:ABO Blood-Group System