title: Down-regulated miRNA-214 induces a cell cycle G1 arrest in gastric cancer cells by up-regulating the PTEN protein.
abstract: To detect the expression of miRNA-214 in human gastric cancer cell lines of BGC823, MKN45 and SGC7901, and to identify the effect of miRNA-214 on cell cycle and apoptosis of these cells. Expression of miRNA-214 in human normal gastric mucosal cell line GES-1 and human gastric cancer cell lines was detected by real-time reverse-transcription polymerase chain reaction. Antisense-miRNA-214 oligonucleotides were transfected transiently into gastric cancer cell lines to down-regulate the expression of miRNA-214. The cell cycle and apoptosis were studied by flow cytometry assay. PTEN, one of the target genes of miRNA-214 was detected by using of immunocytochemistry and Western blotting. MiRNA-214 was overexpressed in gastric cancer cell lines of BGC823, MKN45 and SGC7901 compared with normal gastric mucosal cell line GES-1. Antisense-miRNA-214 oligonucleotides significantly down-regulated the expression of miRNA-214, and increased the portion of G1-phase and decreased the portion of S-phase in BGC823 and MKN45 cells. The immunocytochemistry test and Western blotting analysis showed that the down-regulation of miRNA-214 could significantly up-regulate the expression of PTEN in BGC823 and MKN45 cells. MiRNA-214 is overexpressed in human gastric cancer cell lines of BGC823, MKN45 and SGC7901. The down-regulation of miRNA-214 could induce a G1 cell cycle arrest in them, the up-regulation of PTEN maybe one of the mechanism.
authors.affiliation: Department of Pathology, Xiangya Basic Medical College, Central South University, Changsha 410013, Hunan Province, China. hedwig1112@163.com
authors.forename: Xin
authors.initials: X
authors.lastname: Xiong
authors.forename: Hong-Zheng
authors.initials: HZ
authors.lastname: Ren
authors.forename: Min-Hua
authors.initials: MH
authors.lastname: Li
authors.forename: Jin-Hong
authors.initials: JH
authors.lastname: Mei
authors.forename: Ji-Fang
authors.initials: JF
authors.lastname: Wen
authors.forename: Chang-Li
authors.initials: CL
authors.lastname: Zheng
mesh_terms: D017209:Apoptosis / Q000235:genetics; D015153:Blotting, Western; D059447:Cell Cycle Checkpoints / Q000235:genetics*; D002460:Cell Line; D045744:Cell Line, Tumor; D015536:Down-Regulation; D016193:G1 Phase / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D016376:Oligonucleotides, Antisense / Q000235:genetics; D051059:PTEN Phosphohydrolase / Q000096:biosynthesis / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D016196:S Phase / Q000235:genetics; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism; D014162:Transfection; D015854:Up-Regulation
chemical_list: D035683:MicroRNAs; D016376:Oligonucleotides, Antisense; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human