title: Maize ZmMEK1 is a single-copy gene.
abstract: Mitogen-activated protein kinase (MAPK) cascade constitutes a conserved signaling module in eukaryotes. MAPK kinase (MAPKK) plays a crucial role in a MAPK cascade. ZmMEK1 is the first characterized MAPKK gene in maize. Although ZmMEK1 has been studied in detail in biochemical level, the genomic organization of ZmMEK1 gene is obscure. In this research, we clarified ZmMEK1 is a single-copy gene in the maize genome. Southern blot analysis using 3' specific region of ZmMEK1 cDNA as a probe revealed the presence of distinct single bands in each lane of EcoRI and HindIII. Although previous Southern blot analysis using full-length ZmMEK1 cDNA as a probe revealed several hybridizing bands, we showed here that all bands come from one genomic fragment corresponding to ZmMEK1 gene. Furthermore, ZmMEK1 was induced by PEG, abscisic acid (ABA), and salicylic acid (SA) and was down-regulated by NaCl.
authors.affiliation: State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, 61 DaiZong Street, Tai'an, 271018 Shandong, China.
authors.forename: Yukun
authors.initials: Y
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhou
authors.forename: Lixia
authors.initials: L
authors.lastname: Liu
authors.forename: Liping
authors.initials: L
authors.lastname: Sun
authors.forename: Maoying
authors.initials: M
authors.lastname: Zhang
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Dequan
authors.initials: D
authors.lastname: Li
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D015152:Blotting, Northern; D015139:Blotting, Southern; D019295:Computational Biology; D018628:Gene Dosage / Q000235:genetics*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D048369:MAP Kinase Kinase 1 / Q000235:genetics*; D011092:Polyethylene Glycols / Q000494:pharmacology; D015183:Restriction Mapping; D020156:Salicylic Acid / Q000494:pharmacology; D003313:Zea mays / Q000235:genetics*
chemical_list: D011092:Polyethylene Glycols; D000040:Abscisic Acid; D048369:MAP Kinase Kinase 1; D020156:Salicylic Acid