title: Bt rice expressing Cry1Ab does not stimulate an outbreak of its non-target herbivore, Nilaparvata lugens.
abstract: In this study, the non-target effects of Bt rice "KMD2" expressing a Cry1Ab protein on the performance of the brown planthopper (BPH), Nilaparvata lugens, over multiple generations were evaluated under laboratory and field conditions. In the laboratory, BPH was reared to observe the impact of the Bt rice as compared to its parental non-Bt cultivar Xiushui 11, while the population dynamics and oviposition performance of BPH were investigated in the field. The survival of BPH nymphs fed Bt and non-Bt rice did not differ significantly. The nymph developmental duration of BPH was significantly delayed by the Bt rice by comparison with the non-Bt rice for the 1st and 2nd but not the 4th generation. Most importantly, the fecundity of BPH on the Bt rice was significantly decreased in every generation when compared with the non-Bt rice. In the field investigations, the population density of BPH nymphs was significantly lower in the Bt rice field. However, the temporal pattern of population dynamics of BPH adults was similar between the Bt and non-Bt rice, presumably due to migratory interference of the adults. In the Bt rice field, the percentage of tillers with eggs and the number of eggs per tiller were also significantly lower from tillering to mature stage. Additionally, Cry1Ab protein could not be detected in guts from single BPH adults. In general, our results suggest that the Bt rice "KMD2" could not stimulate an outbreak of BPH.
authors.affiliation: State Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, College of Agriculture and Biotechnology, Institute of Insect Sciences, Zhejiang University, 310029, Hangzhou, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Chen
authors.forename: Jun-Ce
authors.initials: JC
authors.lastname: Tian
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Qi
authors.initials: Q
authors.lastname: Fang
authors.forename: Zunnu Raen
authors.initials: ZR
authors.lastname: Akhtar
authors.forename: Yu-Fa
authors.initials: YF
authors.lastname: Peng
authors.forename: Hu
authors.initials: H
authors.lastname: Cui
authors.forename: Yu-Yuan
authors.initials: YY
authors.lastname: Guo
authors.forename: Qi-Sheng
authors.initials: QS
authors.lastname: Song
authors.forename: Gong-Yin
authors.initials: GY
authors.lastname: Ye
mesh_terms: D025041:Animal Migration; D000818:Animals; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics*; D004731:Endotoxins / Q000235:genetics*; D005260:Female; D005298:Fertility; D018506:Gene Expression Regulation, Plant; D005813:Genes, Synthetic; D006430:Hemiptera / Q000254:growth & development / Q000472:pathogenicity* / Q000502:physiology; D006460:Hemolysin Proteins / Q000235:genetics*; D060434:Herbivory / Q000502:physiology; D008136:Longevity; D008297:Male; D009758:Nymph / Q000254:growth & development / Q000502:physiology; D012275:Oryza / Q000235:genetics / Q000254:growth & development / Q000378:metabolism / Q000469:parasitology*; D010058:Oviposition; D010270:Parasite Egg Count; D010572:Pest Control, Biological / Q000379:methods; D010935:Plant Diseases / Q000469:parasitology; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development / Q000378:metabolism / Q000469:parasitology; D011156:Population Density; D011157:Population Dynamics; D016019:Survival Analysis
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis