title: Mushroom body efferent neurons responsible for aversive olfactory memory retrieval in Drosophila.
abstract: Aversive olfactory memory is formed in the mushroom bodies in Drosophila melanogaster. Memory retrieval requires mushroom body output, but the manner in which a memory trace in the mushroom body drives conditioned avoidance of a learned odor remains unknown. To identify neurons that are involved in olfactory memory retrieval, we performed an anatomical and functional screen of defined sets of mushroom body output neurons. We found that MB-V2 neurons were essential for retrieval of both short- and long-lasting memory, but not for memory formation or memory consolidation. MB-V2 neurons are cholinergic efferent neurons that project from the mushroom body vertical lobes to the middle superiormedial protocerebrum and the lateral horn. Notably, the odor response of MB-V2 neurons was modified after conditioning. As the lateral horn has been implicated in innate responses to repellent odorants, we propose that MB-V2 neurons recruit the olfactory pathway involved in innate odor avoidance during memory retrieval.
authors.affiliation: Genes and Dynamics of Memory Systems, Neurobiology Unit, CNRS, Ecole Supérieure de Physique et de Chimie Industrielles, Paris, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Séjourné
authors.forename: Pierre-Yves
authors.initials: PY
authors.lastname: Plaçais
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Aso
authors.forename: Igor
authors.initials: I
authors.lastname: Siwanowicz
authors.forename: Séverine
authors.initials: S
authors.lastname: Trannoy
authors.forename: Vladimiros
authors.initials: V
authors.lastname: Thoma
authors.forename: Stevanus R
authors.initials: SR
authors.lastname: Tedjakumala
authors.forename: Gerald M
authors.initials: GM
authors.lastname: Rubin
authors.forename: Paul
authors.initials: P
authors.lastname: Tchénio
authors.forename: Kei
authors.initials: K
authors.lastname: Ito
authors.forename: Guillaume
authors.initials: G
authors.lastname: Isabel
authors.forename: Hiromu
authors.initials: H
authors.lastname: Tanimoto
authors.forename: Thomas
authors.initials: T
authors.lastname: Preat
mesh_terms: D000344:Afferent Pathways / Q000502:physiology; D000704:Analysis of Variance; D000818:Animals; D030801:Animals, Genetically Modified; D001362:Avoidance Learning / Q000502:physiology; D001522:Behavior, Animal; D016827:CD8 Antigens / Q000378:metabolism; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D003213:Conditioning, Psychological; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism; D004331:Drosophila melanogaster; D005786:Gene Expression Regulation / Q000502:physiology; D005968:Glutamate Decarboxylase / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics; D008570:Memory, Short-Term / Q000502:physiology; D011939:Mental Recall / Q000502:physiology*; D008954:Models, Biological; D009046:Motor Neurons / Q000145:classification / Q000502:physiology*; D024521:Mushroom Bodies / Q000166:cytology*; D009812:Odorants; D009833:Olfactory Pathways; D012903:Smell / Q000502:physiology*; D016704:Synapsins / Q000378:metabolism; D013696:Temperature; D013997:Time Factors; D014157:Transcription Factors / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism; D050496:Vesicular Glutamate Transport Proteins / Q000378:metabolism
chemical_list: D016827:CD8 Antigens; D029721:Drosophila Proteins; C557617:GAL4 protein, Drosophila; D016704:Synapsins; D014157:Transcription Factors; D050496:Vesicular Glutamate Transport Proteins; D049452:Green Fluorescent Proteins; D014446:Tyrosine 3-Monooxygenase; D002795:Choline O-Acetyltransferase; D005968:Glutamate Decarboxylase; C402565:glutamate decarboxylase 1