title: Isolation of pluripotent stem cells from human third molar dental pulp.
abstract: Potent stem/progenitor cells have been isolated from normal human dental pulps, termed dental pulp stem cells (DPSCs). However, no study has described the presence of stem cell populations in human dental pulp from the third molar with embryonic phenotypes. The dental pulp tissue was cultured in media with the presence of LIF, EGF, and PDGF. In the present study, we describe a new population of pluripotent stem cells that were isolated from dental pulp (DPPSC). These cells are SSEA-4(+), Oct4(+), Nanog(+), FLK-1(+), HNF3beta(+), Nestin(+), Sox2(+), Lin28(+), c-Myc(+), CD13(+), CD105(+), CD3(-), CD45(-), CD90(low), CD29(+), CD73(low), STRO-1(low) and CD146(-). We have investigated by SEM analysis and q-RT-PCR the capacity of DPPSCs to 3D differentiate in vitro using the Cell Carrier 3D glass scaffold into tissues that have similar characteristics to embryonic mesoderm and endoderm layers. These data would support the use of these cells, which are derived from an easily accessible source and can be used in future regeneration protocols for many tissue types that differentiate from the three embryonic layers.
authors.affiliation: Regenerative Medicine Laboratory, Universitat Internacional de Catalunya, Barcelona, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Atari
authors.forename: M
authors.initials: M
authors.lastname: Barajas
authors.forename: F
authors.initials: F
authors.lastname: Hernández-Alfaro
authors.forename: C
authors.initials: C
authors.lastname: Gil
authors.forename: M
authors.initials: M
authors.lastname: Fabregat
authors.forename: E
authors.initials: E
authors.lastname: Ferrés Padró
authors.forename: L
authors.initials: L
authors.lastname: Giner
authors.forename: N
authors.initials: N
authors.lastname: Casals
mesh_terms: D000293:Adolescent; D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology* / Q000378:metabolism; D005260:Female; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D008875:Middle Aged; D008964:Molar, Third; D054457:Tissue Scaffolds; D055815:Young Adult
chemical_list: D015415:Biomarkers