title: [Detection and genotype analysis of sapovirus associated with sporadic diarrhea in Shenzhen in 2009].
abstract: OBJECTIVE
  To conduct an epidemiological and genotype analysis of sapovirus (SaV) associated with sporadic diarrhea in Shenzhen in the year 2009.
  
  
  METHODS
  A total of 852 fecal samples were collected from sporadic cases of diarrhea in Shenzhen in 2009 and detected by reverse-transcription polymerase chain reaction (RT-PCR) using the primers of SLV5317/5749. The PCR products were analyzed with 1.5% agarose gel electrophoresis and sequenced to construct the phylogenetic tree.
  
  
  RESULTS
  Sixteen samples were found positive for SaV, with a positivity rate of 1.88%. Sequence analysis identified 8 isolates as SaV GI genotype (including 3 SaV GI.1 and 5 SaV GI.2), 7 as SaV GIV genotype, and 1 as GII genotype.
  
  
  CONCLUSIONS
  SaV infection is present in Shenzhen with GI as the predominant genotype. This is the first report of SaV GIV strains in China, which differs from the strains of Anhui-A141 and Beijing-CHN99/BJ360, suggesting the genotypic variety of SaV infection in China.
authors.affiliation: Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China. yange2004333@sina.com
authors.forename: Yan-ge
authors.initials: YG
authors.lastname: Wang
authors.forename: Ya-qing
authors.initials: YQ
authors.lastname: He
authors.forename: Wen-long
authors.initials: WL
authors.lastname: Xiang
authors.forename: Yu-xue
authors.initials: YX
authors.lastname: Liao
authors.forename: Qing
authors.initials: Q
authors.lastname: Chen
authors.forename: Shou-yi
authors.initials: SY
authors.lastname: Yu
authors.forename: Gui-fang
authors.initials: GF
authors.lastname: Hu
mesh_terms: D000328:Adult; D002681:China / Q000453:epidemiology; D003967:Diarrhea / Q000453:epidemiology / Q000821:virology*; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D029341:Sapovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D055815:Young Adult
chemical_list: D012367:RNA, Viral