title: Patient-side assay of lipase activity correlating with pancreatic lipase immunoreactivity in the dog.
abstract: Pancreatitis is a common exocrine pancreatic disease in dogs, and the pancreatic lipase immunoreactivity (PLI) test is used for diagnosis. Enzyme catalytic assay is thought to have low specificity, but a lipase activity assay with increased specificity has been developed in human clinical chemistry. We measured serum lipase activity of 65 client-owned dogs using the newly developed FUJI DRI-CHEM slide and compared the results with their PLI concentrations. The results showed a good correlation (r = 0.91), and the normal and pancreatitis dogs identified based on the PLI values were correctly separated based on lipase activity. The present study suggests that FUJI DRI-CHEM lipase activity would be helpful for diagnosis of pacreatitis in dogs and, in particular, that it can be used as a patient-side assay and contributes to immediate treatment.
authors.affiliation: Department of Veterinary Nursing, School of Veterinary Nursing and Technology, Nippon Veterinary and Life Science University, Musashino, Tokyo 180–8602, Japan. katsumi@nvlu.ac.jp
authors.forename: Katsumi
authors.initials: K
authors.lastname: Ishioka
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Hayakawa
authors.forename: Kentaro
authors.initials: K
authors.lastname: Nakamura
authors.forename: Kaoru
authors.initials: K
authors.lastname: Terashima
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D004283:Dog Diseases / Q000097:blood / Q000175:diagnosis* / Q000201:enzymology; D004285:Dogs; D008049:Lipase / Q000097:blood*; D010195:Pancreatitis / Q000097:blood / Q000175:diagnosis / Q000201:enzymology / Q000662:veterinary*
chemical_list: D015415:Biomarkers; D008049:Lipase