title: Temperature dependent (37-15°C) anaerobic digestion of a trichloroethylene-contaminated wastewater.
abstract: The impact of a trichloroethylene (TCE) contaminated wastewater on the microbial community structure of an anaerobic granular biomass at 15°C compared to 37°C was investigated. Four expanded granular sludge bed (EGSB) bioreactors (R1-R4) were employed in pairs at 37 and 15°C. The influents of one of each pair were supplemented with increasing concentrations of TCE (max. 60 mgl(-1)). At 37°C, stable operation was maintained with 88% COD removal and >99% TCE removal at maximum influent TCE concentrations. R3 performance decreased at influent TCE concentration of 60 mgl(-1), although TCE removal rates of >97% were recorded. Archaeal community analysis via clone library and quantitative polymerase chain reaction (qPCR) analysis, and bacterial community analysis via denaturing gradient gel electrophoresis (DGGE), indicated that temperature resulted in a greater change in community structure than the presence of TCE, and clones related to cold adaptation of biomass were identified at 15°C.
authors.affiliation: Microbial Ecology Laboratory, Microbiology, School of Natural Sciences, National University of Ireland, Galway (NUI, Galway), University Road, Galway, Ireland.
authors.forename: Alma
authors.initials: A
authors.lastname: Siggins
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Enright
authors.forename: Vincent
authors.initials: V
authors.lastname: O'Flaherty
mesh_terms: D000693:Anaerobiosis; D001105:Archaea / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D018533:Biomass; D019149:Bioreactors; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013696:Temperature; D014241:Trichloroethylene / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D012336:RNA, Ribosomal, 16S; D014874:Water Pollutants, Chemical; D014241:Trichloroethylene