title: Radiosensitivity of early and late M-phase HeLa cells isolated by a combination of fluorescent ubiquitination-based cell cycle indicator (Fucci) and mitotic shake-off.
abstract: The mitotic shake-off method revealed the remarkable variation of radiosensitivity of HeLa cells during the cell cycle: M phase shows the greatest radiosensitivity and late S phase the greatest radioresistance. This method harvests all M-phase cells with a round shape, making it impossible to further subdivide M-phase cells. Recently, the fluorescent ubiquitination-based cell cycle indicator (Fucci) was developed; this system basically causes cells in G(1) to emit red fluorescence and other cells to emit green fluorescence. Because the green fluorescence rapidly disappears at late M phase, two-dimensional flow cytometry analysis can usually detect a green(high)/red(low) fraction including S-, G(2)- and early M-phase cells but not a transitional fraction between green(high)/red(low) and green(low)/red(low) including late M-phase cells. However, combining the shake-off method concentrated the transitional fraction, which enabled us to separate early and late M-phase cells without using any drugs. Here we demonstrate for the first time that cells in early M phase are more radiosensitive than those in late M phase, implying that early M phase is the most radiosensitive sub-phase during the cell cycle.
authors.affiliation: Oral Radiation Oncology, Department of Oral Restitution, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan.
authors.forename: Motoshi
authors.initials: M
authors.lastname: Nakayama
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kaida
authors.forename: Shifumi
authors.initials: S
authors.lastname: Deguchi
authors.forename: Kengo
authors.initials: K
authors.lastname: Sakaguchi
authors.forename: Masahiko
authors.initials: M
authors.lastname: Miura
mesh_terms: D002455:Cell Division; D005453:Fluorescence; D006367:HeLa Cells / Q000528:radiation effects*; D006801:Humans; D008938:Mitosis; D011836:Radiation Tolerance; D054875:Ubiquitination