title: Various responses to copper and manganese exposure of Carassius auratus gibelio from two populations.
abstract: The aim of this study was to compare the effect of pro-oxidants copper (Cu(2+), 0.005 and 0.050mg L(-1)) or manganese (Mn(2+), 0.17 and 1.7mg L(-1)) on Carassius auratus gibelio from polluted (B) and unpolluted (Z) sites after exposure for fourteen days. Fish from site B showed high levels of lipid peroxidation (TBARS concentration), lower levels of metallothionein (MT)-related metal, total glutathione (GSH), its redox index, superoxide dismutase (Cu,Zn- and Mn-SOD), glutathione-S-transferase (GST) and cholinesterase (ChE) activities and also higher MT-related thiol concentration in the liver and gills. A common effect of exposure was related to genotoxicity, a decrease in GSH and an increase in microsomal ethoxyresorufin O-deethylase activity in the liver. However, the systems of oxidative stress and biotransformation were more efficient in fish from the polluted site, while the responsivity of MTs in this fish was impaired. Principle Component Analysis separated the subgroups from the unpolluted site and fish loaded by lesser concentrations of metals on the one side, and fish from the polluted site jointly with fish exposed to higher concentrations of metals on the other side. The main distinguishing indices of sites and exposures selected by classification and regression tree (CART) analysis were MT characteristics and genotoxicity.
authors.affiliation: Research Laboratory of Comparative Biochemistry and Molecular Biology, Ternopil National Pedagogical University, M. Kryvonosa Street 2, Ternopil 46027, Ukraine.
authors.forename: Halina I
authors.initials: HI
authors.lastname: Falfushynska
authors.forename: Lesya L
authors.initials: LL
authors.lastname: Gnatyshyna
authors.forename: Oksana B
authors.initials: OB
authors.lastname: Stoliar
authors.forename: Yoon Kwon
authors.initials: YK
authors.lastname: Nam
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002802:Cholinesterases / Q000378:metabolism; D003300:Copper / Q000493:pharmacokinetics / Q000633:toxicity*; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D004781:Environmental Exposure; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D006054:Goldfish / Q000502:physiology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008345:Manganese / Q000493:pharmacokinetics / Q000633:toxicity*; D008668:Metallothionein / Q000378:metabolism; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology; D009152:Mutagenicity Tests; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000633:toxicity; D013482:Superoxide Dismutase / Q000378:metabolism; D014018:Tissue Distribution; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D017382:Reactive Oxygen Species; D014874:Water Pollutants, Chemical; D008345:Manganese; D003300:Copper; D008668:Metallothionein; D019363:Cytochrome P-450 CYP1A1; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; D002802:Cholinesterases; D005978:Glutathione