title: Human HS1BP3 induces cell apoptosis and activates AP-1.
abstract: In the present study, we characterized the function of HS1-binding protein 3 (HS1BP3), which is mutated in essential tremor and may be involved in lymphocyte activation. We found that HS1BP3 localized to the mitochondria and endoplasmic reticulum partially. Overexpression of HS1BP3 induced apoptosis in HEK293T and HeLa cell lines. When these cell lines were transfected with HS1BP3, they exhibited nuclear DNA condensation, externalization of phosphatidylserine (PS), and cleavage of poly ADP ribose polymerase (PARP). Furthermore, suppression of HS1BP3 or HS1 expression attenuates HS1BP3 induced apoptosis. In addition, HS1BP3 enhanced activator protein 1 (AP-1)-mediated transcription in a dose-dependent manner. Therefore, we conclude that HS1BP3 regulates apoptosis via HS1 and stimulates AP-1-mediated transcription.
authors.affiliation: Laboratory of Medical Immunology, School of Basic Medical Science, Peking University Health Science Center, Beijing, China. tpshi@126.com
authors.forename: Taiping
authors.initials: T
authors.lastname: Shi
authors.forename: Jieshi
authors.initials: J
authors.lastname: Xie
authors.forename: Ying
authors.initials: Y
authors.lastname: Xiong
authors.forename: Weiwei
authors.initials: W
authors.lastname: Deng
authors.forename: Jinhai
authors.initials: J
authors.lastname: Guo
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: Dalong
authors.initials: D
authors.lastname: Ma
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D017209:Apoptosis / Q000502:physiology*; D001798:Blood Proteins / Q000235:genetics / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism; D002470:Cell Survival; D004789:Enzyme Activation; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D034622:RNA Interference; D018808:Transcription Factor AP-1 / Q000235:genetics / Q000378:metabolism*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D001798:Blood Proteins; C061909:HCLS1 protein, human; C503777:HS1BP3 protein, human; D009419:Nerve Tissue Proteins; D018808:Transcription Factor AP-1; D011065:Poly(ADP-ribose) Polymerases; D053148:Caspase 3