title: Protective effects on myelosuppression mice treated by three different classic Chinese medicine formulae.
abstract: BACKGROUND
  In order to observe the protective therapeutic action and mechanism of Liuwei Dihuang Decoction, Buzhong Yiqi Decoction, and Compound Danshen Decoction on Myelosuppression induced by cyclophosphamide.
  
  
  METHODS
  The mice model was established by intraperitoneal injected with 100 mg/kg cyclophosphamide by human and mice dose conversion on the 9(th), 11(th), 13(th) days during the experiment. Flow cytometry (FCM) was used for detecting the number of cells and investigating bone marrow cell cycles. Spleen was taken out and the mRNA expression level of thrombopoietin (TPO) and c-Mpl were detected by Q-PCR, and c-Mpl in spleen in order to discuss the mechanism of myelosuppression and the protective effects of traditional Chinese medicine.
  
  
  RESULTS
  Both Liuwei Dihuang Decoction Group and Buzhong Yiqi Decoction Group can accelerate bone marrow hematopoietic stem progenitor cells (HSPCs) in marrow-suppressed mice and enhance cell proliferation by promoting cell cycles from G0/G1 phase to access into S, G2/M phase. And at the same time these Chinese decoctions can increase the mRNA expression level of TPO and c-Mpl in spleen.
  
  
  CONCLUSIONS
  Researched showed that Chinese formula take effect by affecting these genes on myelosuppressed mice.
authors.affiliation: The first two authors contributed equally to this work.
authors.forename: An-Bin
authors.initials: AB
authors.lastname: Zhao
authors.forename: Bin
authors.initials: B
authors.lastname: Yu
authors.forename: Xian-Lin
authors.initials: XL
authors.lastname: Wu
authors.forename: Ke-Jian
authors.initials: KJ
authors.lastname: Cao
authors.forename: En-Qing
authors.initials: EQ
authors.lastname: Li
authors.forename: Qing-Mei
authors.initials: QM
authors.lastname: Li
authors.forename: Xiao-Yin
authors.initials: XY
authors.lastname: Chen