title: mir-35 is involved in intestine cell G1/S transition and germ cell proliferation in C. elegans.
abstract: MicroRNA (miRNA) regulates gene expression in many cellular events, yet functions of only a few miRNAs are known in C. elegans. We analyzed the function of mir-35-41 unique to the worm, and show here that mir-35 regulates the G1/S transition of intestinal cells and germ cell proliferation. Loss of mir-35 leads to a decrease of nuclei numbers in intestine and distal mitotic gonad, while re-introduction of mir-35 rescues the mutant phenotypes. Genetic analysis indicates that mir-35 may act through Rb/E2F and SCF pathways. Further bioinformatic and functional analyses demonstrate that mir-35 targets evolutionally conserved lin-23 and gld-1. Together, our study reveals a novel function of mir-35 family in cell division regulation.
authors.affiliation: The Education Ministry Key Laboratory of Cell Proliferation and Differentiation and the State Key Laboratory of Bio-Membrane and Membrane Bio-Engineering, Peking University, 5 Summer Palace Road, Beijing 100871, China.
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.forename: Pengpeng
authors.initials: P
authors.lastname: Liu
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Qingchun
authors.initials: Q
authors.lastname: Cai
authors.forename: Ge
authors.initials: G
authors.lastname: Gao
authors.forename: Wenxia
authors.initials: W
authors.lastname: Zhang
authors.forename: Zuoyan
authors.initials: Z
authors.lastname: Zhu
authors.forename: Dong
authors.initials: D
authors.lastname: Liu
authors.forename: Qichang
authors.initials: Q
authors.lastname: Fan
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D049109:Cell Proliferation; D004261:DNA Replication; D050684:E2F Transcription Factors / Q000378:metabolism; D044783:F-Box Proteins / Q000235:genetics / Q000378:metabolism; D016193:G1 Phase; D005854:Germ Cells / Q000166:cytology*; D007422:Intestines / Q000166:cytology*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D010641:Phenotype; D016160:Retinoblastoma Protein / Q000378:metabolism; D016196:S Phase
chemical_list: D029742:Caenorhabditis elegans Proteins; D018797:Cell Cycle Proteins; D050684:E2F Transcription Factors; D044783:F-Box Proteins; C094448:GLD-1 protein, C elegans; C570417:MIRN35 microRNA, C elegans; D035683:MicroRNAs; D016160:Retinoblastoma Protein; C418507:lin-23 protein, C elegans