title: Degumming of vegetable oils by a novel phospholipase B from Pseudomonas fluorescens BIT-18.
abstract: Pseudomonas fluorescens BIT-18 was isolated from soil near a vegetable oil factory and shown to produce a B-type phospholipase. The enzyme was partially purified by ammonium sulfate precipitation. Gas chromatography demonstrated that the enzyme preparation hydrolyzed both the 1- and 2-ester bonds of phosphatidylcholine. When degumming of soybean, rapeseed, and peanut oil was performed with this enzyme preparation, oils with phosphorous contents lower than 5mg/kg were obtained after 5h of enzyme treatment at 40°C. The enzyme preparation did not show lipase activity, thus free fatty acids were only generated from the phospholipids. Therefore, this novel phospholipase B is potentially useful for the refining of high-quality oils with attractive yields.
authors.affiliation: School of Life Science, Beijing Institute of Technology, 100081 Beijing, PR China.
authors.forename: Fangyan
authors.initials: F
authors.lastname: Jiang
authors.forename: Jinmei
authors.initials: J
authors.lastname: Wang
authors.forename: Imdad
authors.initials: I
authors.lastname: Kaleem
authors.forename: Dazhang
authors.initials: D
authors.lastname: Dai
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Zhou
authors.forename: Chun
authors.initials: C
authors.lastname: Li
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel; D008245:Lysophospholipase / Q000378:metabolism*; D010938:Plant Oils / Q000378:metabolism*; D011551:Pseudomonas fluorescens / Q000201:enzymology*
chemical_list: D010938:Plant Oils; D008245:Lysophospholipase