title: [Effective method for isolation of total RNA from Fagopyrum cymosum callus].
abstract: OBJECTIVE
  To establish a method for isolation of the total RNA from Fagopyrum cymosum callus.
  
  
  METHODS
  The improved method combining that of CTAB extraction with the LiCl precipitation was used to isolate the total RNA from the four F. cymosum callus. The quality of the RNA was detected by UV spectrophotometric analysis, 0.8% non-denaturing agarose gel electrophoresis and RNA reverse transcription.
  
  
  RESULTS
  The bands of 28S and 18S could be seen clearly by agarose gel electrophoresis, and the value of A260/A280 was between 1.9 and 2.0. The cDNA which was reverse-transcribed by the total RNA showed a wide length rage of 500 bp-5 kb.
  
  
  CONCLUSIONS
  The RNA extracted by this method meets the requirement of reverse transcription-polymerase chain reaction (RT-PCR), construction of cDNA libraries, et al. This improved method can be used to isolate the total RNA from F. cymosum callus with the advantage of simpleness, efficiency and low cost.
authors.affiliation: College of Horticulture and Landscape, Southwest University, Chongqing Engineering Research Center for Floriculture, Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, Chongqing 400715, China.
authors.forename: Shunzhao
authors.initials: S
authors.lastname: Sui
authors.forename: Jing
authors.initials: J
authors.lastname: Ma
authors.forename: Yandong
authors.initials: Y
authors.lastname: Li
authors.forename: Qinlong
authors.initials: Q
authors.lastname: Zhu
authors.forename: Mingyang
authors.initials: M
authors.lastname: Li
mesh_terms: D019612:Fagopyrum / Q000235:genetics* / Q000254:growth & development; D018749:RNA, Plant / Q000032:analysis / Q000302:isolation & purification*
chemical_list: D018749:RNA, Plant