title: Cross-linking measurements of in vivo protein complex topologies.
abstract: Identification and measurement of in vivo protein interactions pose critical challenges in the goal to understand biological systems. The measurement of structures and topologies of proteins and protein complexes as they exist in cells is particularly challenging, yet critically important to improve understanding of biological function because proteins exert their intended function only through the structures and interactions they exhibit in vivo. In the present study, protein interactions in E. coli cells were identified in our unbiased cross-linking approach, yielding the first in vivo topological data on many interactions and the largest set of identified in vivo cross-linked peptides produced to date. These data show excellent agreement with protein and complex crystal structures where available. Furthermore, our unbiased data provide novel in vivo topological information that can impact understanding of biological function, even for cases where high resolution structures are not yet available.
authors.affiliation: Department of Chemistry, Washington State University, Pullman, WA 99164, USA.
authors.forename: Chunxiang
authors.initials: C
authors.lastname: Zheng
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.forename: Michael R
authors.initials: MR
authors.lastname: Hoopmann
authors.forename: Jimmy K
authors.initials: JK
authors.lastname: Eng
authors.forename: Xiaoting
authors.initials: X
authors.lastname: Tang
authors.forename: Chad R
authors.initials: CR
authors.lastname: Weisbrod
authors.forename: James E
authors.initials: JE
authors.lastname: Bruce
mesh_terms: D001665:Binding Sites; D002411:Cation Exchange Resins / Q000737:chemistry; D002853:Chromatography, Liquid; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004926:Escherichia coli / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D013058:Mass Spectrometry; D008968:Molecular Conformation; D046912:Multiprotein Complexes / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D060066:Protein Interaction Maps
chemical_list: D002411:Cation Exchange Resins; D003432:Cross-Linking Reagents; D046912:Multiprotein Complexes; D010455:Peptides