title: Poly(I:C)-induced tumour cell death leads to DC maturation and Th1 activation.
abstract: Dendritic cells (DCs) have the ability to generate peptide epitopes for MHC class I molecules derived from apoptotic tumour cells for direct recognition by cytotoxic T cells. This function has lead to DCs being used in vaccine strategies. In this study, we investigate the effect of inducing apoptosis in tumour cell lines using IFN-γ and poly(I:C), the subsequent maturation of the endocytosing DC and its ability to direct the resulting T cell response. We show that uptake of poly(I:C)-induced apoptotic tumour cells leads to DC maturation and activation with a Th1 cell polarising capacity. In contrast, these effects are not seen by DCs loaded with γ-irradiated apoptotic tumour cells. We propose that the manner in which tumour cells are induced to die can have a profound effect on the endocytosing DC and the resulting T cell response.
authors.affiliation: Systems Immunology Group, Cellular and Molecular Medicine, St George's University of London, UK.
authors.forename: Edit
authors.initials: E
authors.lastname: Kovalcsik
authors.forename: Katie
authors.initials: K
authors.lastname: Lowe
authors.forename: Mike
authors.initials: M
authors.lastname: Fischer
authors.forename: Angus
authors.initials: A
authors.lastname: Dalgleish
authors.forename: Mark D
authors.initials: MD
authors.lastname: Bodman-Smith
mesh_terms: D017209:Apoptosis / Q000276:immunology; D016923:Cell Death / Q000276:immunology; D045744:Cell Line, Tumor; D016207:Cytokines / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology* / Q000473:pathology; D006801:Humans; D008285:Major Histocompatibility Complex / Q000276:immunology; D008545:Melanoma / Q000276:immunology* / Q000473:pathology; D011070:Poly I-C / Q000276:immunology / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D018417:Th1 Cells / Q000276:immunology*
chemical_list: D016207:Cytokines; D011070:Poly I-C