title: Expression of two novel cytochrome P450 3A131 and 3A132 in liver and small intestine of domestic cats.
abstract: Cytochrome P450 3A (CYP3A) is the major subfamily of CYP, one of the most important metabolizing enzymes for drugs in humans and other mammals. We found two novel CYP3A genes, CYP3A131 and CYP3A132 in domestic cats (Felis catus). Both feline CYP3A proteins consist of 504 deduced amino acids and show high identity with canine CYP3A homologues and those of some artiodactyls. CYP3A131 transcripts were expressed predominantly in liver and small intestine, and to a negligible extent in other tissues, including brain, heart, kidney and lung. CYP3A132 expression was only detected in liver with much lesser amount. These results suggest the possible major role of CYP3A131 in xenobiotic metabolism including first-pass effects in domestic cats.
authors.affiliation: School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069–8501, Japan.
authors.forename: Kouichi
authors.initials: K
authors.lastname: Honda
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Komatsu
authors.forename: Fumika
authors.initials: F
authors.lastname: Koyama
authors.forename: Akira
authors.initials: A
authors.lastname: Kubota
authors.forename: Kei
authors.initials: K
authors.lastname: Kawakami
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Asakura
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Uno
authors.forename: Takio
authors.initials: T
authors.lastname: Kitazawa
authors.forename: Takeo
authors.initials: T
authors.lastname: Hiraga
authors.forename: Hiroki
authors.initials: H
authors.lastname: Teraoka
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002415:Cats; D051544:Cytochrome P-450 CYP3A / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018076:DNA, Complementary / Q000032:analysis / Q000235:genetics; D005260:Female; D007421:Intestine, Small / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA / Q000662:veterinary
chemical_list: D018076:DNA, Complementary; D003577:Cytochrome P-450 Enzyme System; D051544:Cytochrome P-450 CYP3A