title: Distribution of acute phase proteins in the bovine forestomachs and abomasum.
abstract: Acute phase proteins (APPs) are produced mainly by the liver and their concentration is increased during the systemic inflammatory response. Expression of haptoglobin (Hp), serum amyloid A (SAA), lipopolysaccharide-binding protein (LBP) and α-1 acid glycoprotein (AGP) was determined in the mucosa of the normal bovine forestomachs and abomasum by qualitative and quantitative reverse transcriptase-PCR for mRNA and by Western blot analysis and immunohistochemistry for proteins. Although expression of SAA mRNA was evident in the forestomachs and abomasum, SAA protein was identified only in the abomasum. Expression of Hp protein was high in the forestomachs and abomasum, even though expression of Hp mRNA was negligible. The main site of expression of LBP mRNA was the omasum, whereas the highest protein expression was evident in the abomasum. AGP was expressed at low levels in the bovine forestomachs. Western blot analysis revealed a heterogeneous electrophoretic pattern for AGP, LBP and Hp, indicating that different stomach compartments produce isoforms that are different to those expressed by the liver. Expression of APPs by the bovine forestomachs and abomasum may contribute to regulation of the innate immune response against pathogens.
authors.affiliation: Università degli Studi di Milano, Dipartimento di Patologia Animale, Igiene e Sanità Pubblica Veterinaria, via Celoria 10, 20133 Milano, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Dilda
authors.forename: Laura Francesca
authors.initials: LF
authors.lastname: Pisani
authors.forename: Mizanur Md
authors.initials: MM
authors.lastname: Rahman
authors.forename: Silvia
authors.initials: S
authors.lastname: Modina
authors.forename: Irene
authors.initials: I
authors.lastname: Tessaro
authors.forename: Paola
authors.initials: P
authors.lastname: Sartorelli
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Ceciliani
authors.forename: Cristina
authors.initials: C
authors.lastname: Lecchi
mesh_terms: D000209:Acute-Phase Proteins / Q000032:analysis / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western / Q000662:veterinary; D002417:Cattle / Q000378:metabolism*; D005753:Gastric Mucosa / Q000378:metabolism; D007150:Immunohistochemistry / Q000662:veterinary; D009928:Organ Specificity; D020033:Protein Isoforms / Q000032:analysis / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D013279:Stomach, Ruminant / Q000378:metabolism*
chemical_list: D000209:Acute-Phase Proteins; D020033:Protein Isoforms; D012333:RNA, Messenger