title: 31-Phosphorus magnetic resonance spectroscopy for dynamic assessment of adenosine triphosphate levels in pancreas preserved by the two-layer method.
abstract: Cold preservation injury influences islet graft function. Reliable tools for real-time assessment of pancreas viability before islet isolation are lacking. Phosphorus magnetic resonance spectroscopy ((31)P-MRS) was used immediately after organ harvest to study rat pancreases at 4 °C to 6 °C in five randomized preservation groups: Marshall's solution, static two-layer method (TLM), continuous TLM with oxygen perfused at 0.5 L/min, and static TLM or continuous TLM both the latter following 30 minutes of warm ischemia (WI). (31)P spectra were analyzed for phosphomonoesters, inorganic phosphate (Pi) and α-, β-and γ-nucleotide triphosphate. Intergroup rates of change of [γ-adenosine triphosphate (ATP)]/[Pi] and [β-ATP]/[Pi] throughout preservation period were significantly different. For continuous TLM there was an increase relative to baseline (0.043 (SD0.033) h(-1) and 0.029 (0.029) h(-1), respectively) but a decrease for both static TLM (-0.023 (0.016) h(-1) and 0.015 (0.026), P < .001 and < .05, respectively) and Marshall's (-0.049 (0.025) h(-1) and -0.036 (0.019) h(-1), respectively, both P < .001) with respect to continuous TLM. Rate of decrease was similar for the Marshall's and static TLM groups. [γ-ATP]/[Pi] and [β-ATP]/[Pi] increased with WI continuous TLM (0.008 [0.009] h(-1) and 0.007 [0.008] hr(-1), respectively) but decreased for WI static TLM (-0.018 (0.008) h(-1) and -0.014 (0.004) hr(-1), respectively, P < .001). (31)P-MRS is an effective tool for noninvasive assessment of pancreas bioenergetics. Continuous TLM preserves cellular bioenergetics and is superior to current non-perfluorocar bone based solutions for pancreas preservation.
authors.affiliation: Department of Surgery, Royal Free Hospital and University College School of Medicine, London, England. agrawal_aditya@hotmail.com
authors.forename: A
authors.initials: A
authors.lastname: Agrawal
authors.forename: A
authors.initials: A
authors.lastname: Bainbridge
authors.forename: S
authors.initials: S
authors.lastname: Powis
authors.forename: B
authors.initials: B
authors.lastname: Fuller
authors.forename: E B
authors.initials: EB
authors.lastname: Cady
authors.forename: B R
authors.initials: BR
authors.lastname: Davidson
mesh_terms: D000255:Adenosine Triphosphate / Q000097:blood*; D000818:Animals; D015925:Cryopreservation; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D010179:Pancreas; D010759:Phosphorus Isotopes; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D010759:Phosphorus Isotopes; D000255:Adenosine Triphosphate