title: Identification of a novel benzimidazole that inhibits bacterial biofilm formation in a broad-spectrum manner.
abstract: Bacterial biofilm formation causes significant industrial economic loss and high morbidity and mortality in medical settings. Biofilms are defined as multicellular communities of bacteria encased in a matrix of protective extracellular polymers. Because biofilms have a high tolerance for treatment with antimicrobials, protect bacteria from immune defense, and resist clearance with standard sanitation protocols, it is critical to develop new approaches to prevent biofilm formation. Here, a novel benzimidazole molecule, named antibiofilm compound 1 (ABC-1), identified in a small-molecule screen, was found to prevent bacterial biofilm formation in multiple Gram-negative and Gram-positive bacterial pathogens, including Pseudomonas aeruginosa and Staphylococcus aureus, on a variety of different surface types. Importantly, ABC-1 itself does not inhibit the growth of bacteria, and it is effective at nanomolar concentrations. Also, coating a polystyrene surface with ABC-1 reduces biofilm formation. These data suggest ABC-1 is a new chemical scaffold for the development of antibiofilm compounds.
authors.affiliation: Department of Microbiology, 5180 Biomedical Physical Sciences, Michigan State University, East Lansing, MI 48824, USA.
authors.forename: Karthik
authors.initials: K
authors.lastname: Sambanthamoorthy
authors.forename: Ankush A
authors.initials: AA
authors.lastname: Gokhale
authors.forename: Weiwei
authors.initials: W
authors.lastname: Lao
authors.forename: Vijay
authors.initials: V
authors.lastname: Parashar
authors.forename: Matthew B
authors.initials: MB
authors.lastname: Neiditch
authors.forename: Martin F
authors.initials: MF
authors.lastname: Semmelhack
authors.forename: Ilsoon
authors.initials: I
authors.lastname: Lee
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Waters
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D001562:Benzimidazoles / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D001562:Benzimidazoles; C031000:benzimidazole