title: Distribution and accumulation of liposomal form of doxorubicin in breast cancer cells of MCF-7 line.
abstract: OBJECTIVE
  To study distribution and accumulation of liposomal form of doxorubicin in human breast cancer cells of MCF-7 line and Dox-resistant subline MCF-7/Dox.
  
  
  METHODS
  High performance liquid chromatography and laser confocal microscopy were used.
  
  
  RESULTS
  It has been shown that conventional form of doxorubicin was more efficiently delivered to the MCF-7 cells already after 30 min of incubation amounting to its maximum concentration after 4 h. MCF-7/Dox cells are characterized by lower doxorubicin accumulation rate compared with parental cells. The quantity of accumulated liposomal form of doxorubicin is high in MCF-7 cells, and, what is important, Dox-resistant cells accumulated higher levels of liposomal form of doxorubicin than its conventional form.
  
  
  CONCLUSIONS
  It has been shown that intracellular distribution and accumulation of liposomal forms of doxorubicin in parental and Dox-resistant MCF-7 cells differs from that of conventional doxorubicin.
authors.affiliation: R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv 03022, Ukraine. narusha@ukr.net
authors.forename: N V
authors.initials: NV
authors.lastname: Rusetskaya
authors.forename: N
authors.initials: N
authors.lastname: Khariton
authors.forename: O V
authors.initials: OV
authors.lastname: Yurchenko
authors.forename: V F
authors.initials: VF
authors.lastname: Chekhun
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism*; D045744:Cell Line, Tumor; D002851:Chromatography, High Pressure Liquid; D004317:Doxorubicin / Q000378:metabolism*; D005260:Female; D006801:Humans; D008081:Liposomes; D018613:Microscopy, Confocal
chemical_list: D008081:Liposomes; D004317:Doxorubicin