title: Mutational patterns in RNA secondary structure evolution examined in three RNA families.
abstract: The goal of this work was to study mutational patterns in the evolution of RNA secondary structure. We analyzed bacterial tmRNA, RNaseP and eukaryotic telomerase RNA secondary structures, mapping structural variability onto phylogenetic trees constructed primarily from rRNA sequences. We found that secondary structures evolve both by whole stem insertion/deletion, and by mutations that create or disrupt stem base pairing. We analyzed the evolution of stem lengths and constructed substitution matrices describing the changes responsible for the variation in the RNA stem length. In addition, we used principal component analysis of the stem length data to determine the most variable stems in different families of RNA. This data provides new insights into the evolution of RNA secondary structures and patterns of variation in the lengths of double helical regions of RNA molecules. Our findings will facilitate design of improved mutational models for RNA structure evolution.
authors.affiliation: Institute of Bioinformatics, University of Georgia, Athens, Georgia, United States of America. anuj@uga.edu
authors.forename: Anuj
authors.initials: A
authors.lastname: Srivastava
authors.forename: Liming
authors.initials: L
authors.lastname: Cai
authors.forename: Jan
authors.initials: J
authors.lastname: Mrázek
authors.forename: Russell L
authors.initials: RL
authors.lastname: Malmberg
mesh_terms: D019143:Evolution, Molecular; D009154:Mutation; D009690:Nucleic Acid Conformation; D025341:Principal Component Analysis; D012313:RNA / Q000737:chemistry*
chemical_list: D012313:RNA