title: Effect of natural honey on human platelets and blood coagulation proteins.
abstract: Present study was conducted to determine the effects of honey on blood hemostasis, in-vitro effect of honey was observed on platelet aggregation and blood coagulation employing, activated partial prothrombin time (aPTT), prothrombin time (PT), thrombin time (TT) and fibrinogen levels in blood. Honey samples showed moderate inhibition of platelet aggregation with IC(50) 5-7.5%. The coagulation assays showed that at higher concentrations (>15%) honey samples increased whole blood clotting time. When assayed in platelet poor plasma (PPP), honey samples significantly (P>0.005) prolonged aPTT, PT, and TT. The honey samples (at 3.75% and 7.5% concentrations) cause mean increment of aPTT = 19±10% and 62±10%; PT 6±5% and 40±5%; TT 35±15% and 112±30% respectively. Moreover, PPP isolated from whole blood pre-incubated with honey samples (9.0% for 10 minutes) showed mean prolongation of aPTT, PT and TT of 45±21%, 26±9% and 105±24% respectively. Interestingly, incubation of honey at 6.25% and 11.75% concentrations in PPP considerably (P≥0.005) reduced fibrinogen levels i.e. 13±4% and 86±30% respectively. The present study outlines the inhibitory effect of natural honey on platelet aggregation and blood coagulation. These observations provide first line data for modulatory role(s) of honey on process of hemostasis.
authors.affiliation: Department of Pharmacology, Baqai Medical University, Karachi, Pakistan.
authors.forename: Asif
authors.initials: A
authors.lastname: Ahmed
authors.forename: Rafeeq Alam
authors.initials: RA
authors.lastname: Khan
authors.forename: M Kamran
authors.initials: MK
authors.lastname: Azim
authors.forename: S Arshad
authors.initials: SA
authors.lastname: Saeed
authors.forename: M Ahmed
authors.initials: MA
authors.lastname: Mesaik
authors.forename: Shakil
authors.initials: S
authors.lastname: Ahmed
authors.forename: Imran
authors.initials: I
authors.lastname: Imran
mesh_terms: D001780:Blood Coagulation Tests / Q000379:methods*; D001792:Blood Platelets / Q000187:drug effects*; D005340:Fibrinogen / Q000378:metabolism*; D006487:Hemostasis / Q000187:drug effects*; D006722:Honey / Q000009:adverse effects* / Q000032:analysis; D006801:Humans; D010949:Plasma / Q000187:drug effects / Q000378:metabolism
chemical_list: D005340:Fibrinogen