title: Survival of infectious prions in water.
abstract: The objective of this study was to evaluate the fate of infectious prions in water. Known concentrations of infectious prions were added to deionized water, tap water, and wastewater. Samples were incubated at 25°C, 37°C, and 50°C for 1 to 8 weeks. The standard scrapie cell assay (SSCA) which includes the ELISPOT (Enzyme Linked Immuno-Spot) reaction was performed to determine prion infectivity and quantity as a function of time. A reduction of infectious prions was observed at 25°C, 37°C, and 50°C ranging between 0.5-log₁₀ and 1.4-log₁₀ in one week. Results suggest that organic matter was instrumental in protecting infectious prions, allowing them to remain infectious for a longer period of time. Thus, our data effectively show a quantifiable reduction of infectious prions in water and identifies some of the components that may influence infectivity.
authors.affiliation: Soil, Water, and Environmental Science Department, University of Arizona, Tucson, Arizona 85756, USA. smiles@email.arizona.edu
authors.forename: Syreeta L
authors.initials: SL
authors.lastname: Miles
authors.forename: Kazue
authors.initials: K
authors.lastname: Takizawa
authors.forename: Charles P
authors.initials: CP
authors.lastname: Gerba
authors.forename: Ian L
authors.initials: IL
authors.lastname: Pepper
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D058501:Enzyme-Linked Immunospot Assay; D006801:Humans; D017096:Prion Diseases / Q000453:epidemiology / Q000382:microbiology* / Q000401:mortality; D011328:Prions / Q000032:analysis* / Q000302:isolation & purification; D012608:Scrapie / Q000453:epidemiology / Q000635:transmission; D012722:Sewage / Q000032:analysis* / Q000737:chemistry / Q000382:microbiology; D015996:Survival Rate; D013696:Temperature; D013997:Time Factors; D014867:Water / Q000032:analysis*; D014871:Water Microbiology
chemical_list: D011328:Prions; D012722:Sewage; D014867:Water