title: The role of p73 G4C14-to-A4T14 polymorphism in the susceptibility to cervical cancer.
abstract: Several reports have suggested the importance of p73 polymorphisms in tumor behavior. We investigated the role of a p73 gene polymorphism in the susceptibility to cervical lesions in a southwestern European population. Peripheral blood samples were obtained from Radiotherapy and Gynaecology Departments of Portuguese Institute of Oncology (Porto, Portugal), from 1998 to 2002. We analyzed the p73 cytosine thymine polymorphism in peripheral blood DNA of 176 healthy donors, 38 high-grade squamous intraepithelial lesions (HSIL), and 141 patients with primary untreated invasive cervical cancers (ICC), by polymerase chain reaction-restriction fragment length polymorphism. Our results demonstrate a twofold increased susceptibility to the development of HSIL in women carrying the p73 AT allele (OR=2.39; p=0.022). Further, this association seems to be more evident in women with high parity (OR=12.53; p=0.007). This is in agreement with the possible role of p73 in cervical carcinogenesis, namely, in human papillomavirus-infected transition zone subjected to the action of estrogens and in conjunction with disruption of differentiation program of this squamous epithelium that occurs in HSIL phase before the next step to invasiveness and squamous cervical cancer.
authors.affiliation: Radiotherapy, Gynecology, and Oncology Departments, Instituto Português de Oncologia, Porto, Portugal.
authors.forename: Rogéria
authors.initials: R
authors.lastname: Craveiro
authors.forename: Isabel
authors.initials: I
authors.lastname: Bravo
authors.forename: Raquel
authors.initials: R
authors.lastname: Catarino
authors.forename: Ana Luisa
authors.initials: AL
authors.lastname: Teixeira
authors.forename: Hugo
authors.initials: H
authors.lastname: Sousa
authors.forename: Deolinda
authors.initials: D
authors.lastname: Pereira
authors.forename: Helena
authors.initials: H
authors.lastname: Pereira
authors.forename: Rui
authors.initials: R
authors.lastname: Medeiros
mesh_terms: D020856:AT Rich Sequence / Q000235:genetics; D000328:Adult; D000368:Aged; D001482:Base Composition / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000453:epidemiology / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D020862:GC Rich Sequence / Q000235:genetics; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D060787:Neoplasm Grading; D009687:Nuclear Proteins / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000502:physiology*; D000072160:Tumor Protein p73; D025521:Tumor Suppressor Proteins / Q000235:genetics*; D002583:Uterine Cervical Neoplasms / Q000453:epidemiology / Q000235:genetics* / Q000473:pathology; D002578:Uterine Cervical Dysplasia / Q000453:epidemiology / Q000235:genetics* / Q000473:pathology
chemical_list: D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; C000606460:TP73 protein, human; D000072160:Tumor Protein p73; D025521:Tumor Suppressor Proteins