title: Rapid separation of triacylglycerol positional isomers binding two saturated Fatty acids using octacocyl silylation column.
abstract: The rapid separation of a triacylglycerol positional isomer (TAG-PI) pair was examined via high-performance liquid chromatography-atmospheric pressure chemical ionization tandem mass spectrometry using an octacocyl silylation (C28) column. A TAG-PI pair binding two palmitic acids and one fatty acid that structurally differs from palmitic acid was separated at 10°C and 15°C using acetone as the mobile phase. However, the TAG-PI pair binding two unsaturated fatty acids and one saturated fatty acid was not separated by the C28 column. The results indicate that the structures of the two palmitic acids (saturated fatty acids) and the other fatty acid at the α or β position in TAG play an important role in the separation of the TAG-PI pair, and that the structure of the fatty acid needs to be considerably different from that of the palmitic acid, specifically in terms of the chain length or the location of the double bond.
authors.affiliation: Tsukishima Foods Industry Co., Ltd., Kigashi Kasai, Edogawa-ku, Tokyo, Japan. t-nagai@tsukishima.co.jp
authors.forename: Toshiharu
authors.initials: T
authors.lastname: Nagai
authors.forename: Naohiro
authors.initials: N
authors.lastname: Gotoh
authors.forename: Hoyo
authors.initials: H
authors.lastname: Mizobe
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Yoshinaga
authors.forename: Koichi
authors.initials: K
authors.lastname: Kojima
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Matsumoto
authors.forename: Shun
authors.initials: S
authors.lastname: Wada
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D005227:Fatty Acids / Q000737:chemistry / Q000302:isolation & purification*; D007536:Isomerism; D010169:Palmitic Acids / Q000737:chemistry / Q000302:isolation & purification; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods; D013997:Time Factors; D014280:Triglycerides / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D005227:Fatty Acids; D010169:Palmitic Acids; D014280:Triglycerides; C026679:octadecylsilica; D012822:Silicon Dioxide