title: Oxidative stress responses in tilapia (Oreochromis niloticus) exposed to a single dose of pure cylindrospermopsin under laboratory conditions: influence of exposure route and time of sacrifice.
abstract: Cylindrospermopsin (CYN) is a toxin produced by various cyanobacterial species that are increasingly being found in freshwater systems. Although CYN can have toxic effects in humans, domestic animals and wildlife, it has been subject to very little investigation (particularly in fish). It has been reported to deplete the cellular glutathione content but the role of oxidative stress in the pathogenicity of CYN in fish is unknown. For this reason tilapia fish were exposed to 200 μg/kg pure CYN through two different exposure routes-gavage and intraperitoneal injection-and sacrificed after 24 h and 5 days. The results showed an increase in NADPH oxidase activity (a biomarker of reactive oxygen species formation), lipid peroxidation (LPO) and protein oxidation; no changes in DNA oxidation; and a reduction in glutathione levels (GSH) and γ-glutamylcysteine synthetase (GCS) activity, the limiting enzyme in glutathione synthesis. The time of sacrifice had a bigger influence on the results than the exposure route because after 5 days some of the biomarkers assayed had recovered their pre-intoxication levels, which was not the case after 24 h.
authors.affiliation: Area of Toxicology, Faculty of Pharmacy, Profesor García González 2, 41012 Seville, Spain.
authors.forename: Daniel
authors.initials: D
authors.lastname: Gutiérrez-Praena
authors.forename: Angeles
authors.initials: A
authors.lastname: Jos
authors.forename: Silvia
authors.initials: S
authors.lastname: Pichardo
authors.forename: Ana M
authors.initials: AM
authors.lastname: Cameán
mesh_terms: D000470:Alkaloids; D000818:Animals; D001427:Bacterial Toxins; D015415:Biomarkers / Q000378:metabolism; D023681:Cichlids; D000087522:Cyanobacteria Toxins; D004305:Dose-Response Relationship, Drug; D005721:Glutamate-Cysteine Ligase / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D007753:Laboratories; D019255:NADPH Oxidases / Q000378:metabolism; D018384:Oxidative Stress; D014498:Uracil / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000633:toxicity; D014874:Water Pollutants, Chemical / Q000008:administration & dosage / Q000633:toxicity*
chemical_list: D000470:Alkaloids; D001427:Bacterial Toxins; D015415:Biomarkers; D000087522:Cyanobacteria Toxins; D014874:Water Pollutants, Chemical; C089595:cylindrospermopsin; D014498:Uracil; D019255:NADPH Oxidases; D005721:Glutamate-Cysteine Ligase; D005978:Glutathione