title: Disruption of LRRK2 does not cause specific loss of dopaminergic neurons in zebrafish.
abstract: Mutations in LRRK2 are genetically linked to Parkinson's disease (PD) but its normal biological function is largely unknown. Sheng et al. recently reported that deletion of the WD40 domain of LRRK2 in zebrafish specifically causes PD-like loss of neurons and behavior defect. However, our similar early study and recent confirming experiments using the same reagents reported by Sheng et al. failed to reproduce the phenotype of the loss of dopaminergic neurons, although the mRNA of LRRK2 was molecularly disrupted. Our study suggests that function of LRRK2 and its usefulness to generate zebrafish PD model needs further evaluation.
authors.affiliation: Laboratory of Chemical Genomics, School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, Shenzhen, China.
authors.forename: Guiqi
authors.initials: G
authors.lastname: Ren
authors.forename: Shengchang
authors.initials: S
authors.lastname: Xin
authors.forename: Song
authors.initials: S
authors.lastname: Li
authors.forename: Hanbing
authors.initials: H
authors.lastname: Zhong
authors.forename: Shuo
authors.initials: S
authors.lastname: Lin
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004298:Dopamine / Q000378:metabolism*; D055785:Gene Knockdown Techniques; D017403:In Situ Hybridization; D000071158:Leucine-Rich Repeat Serine-Threonine Protein Kinase-2; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015027:Zebrafish / Q000196:embryology; D029961:Zebrafish Proteins / Q000235:genetics / Q000502:physiology*
chemical_list: D017931:DNA Primers; D029961:Zebrafish Proteins; C551314:LRRK2 protein, zebrafish; D000071158:Leucine-Rich Repeat Serine-Threonine Protein Kinase-2; D017346:Protein Serine-Threonine Kinases; D004298:Dopamine