title: Qualitative and quantitative determination of ten iridoids and secoiridoids in Gentiana straminea Maxim. by LC-UV-ESI-MS.
abstract: A simple and accurate HPLC-UV/MS method was developed for the simultaneous determination of ten iridoids and secoiridoids in the roots of Gentiana straminea Maxim. Separations were performed on a Kromasil-C18 column by gradient elution using methanol and water containing phosphoric acid. Analytes were identified by HPLC coupled with ESI-MS experiments. The chromatographic method was validated for selectivity, linearity, precision, limit of detection, limit of quantification, accuracy, and stability. The developed assay could be considered as a suitable quality control method for G. straminea and other "Qinjiao" herbs.
authors.affiliation: College of Life Science and Technology, Beijing University of Chemical Technology, No. 15 Beisanhuan East Road, Beijing 100029, People's Republic of China.
authors.forename: Shihu
authors.initials: S
authors.lastname: Wei
authors.forename: Peicheng
authors.initials: P
authors.lastname: Zhang
authors.forename: Xizhi
authors.initials: X
authors.lastname: Feng
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kodama
authors.forename: Changyuan
authors.initials: C
authors.lastname: Yu
authors.forename: Guang
authors.initials: G
authors.lastname: Chen
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification / Q000592:standards; D030012:Gentiana / Q000737:chemistry; D039823:Iridoids / Q000032:analysis*; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification / Q000592:standards; D018517:Plant Roots; D010946:Plants, Medicinal; D011786:Quality Control; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization; D013056:Spectrophotometry, Ultraviolet
chemical_list: D004365:Drugs, Chinese Herbal; D039823:Iridoids; D010936:Plant Extracts