title: Overexpression of the trehalose-6-phosphate synthase gene OsTPS1 enhances abiotic stress tolerance in rice.
abstract: Trehalose plays an important role in metabolic regulation and abiotic stress tolerance in a variety of organisms. In plants, its biosynthesis is catalyzed by two key enzymes: trehalose-6-phosphate synthase (TPS) and trehalose-6-phosphate phosphatase (TPP). The genome of rice (Oryza sativa) contains 11 OsTPS genes, and only OsTPS1 shows TPS activity. To demonstrate the physiological function of OsTPS1, we introduced it into rice and found that OsTPS1 overexpression improved the tolerance of rice seedling to cold, high salinity and drought treatments without other significant phenotypic changes. In transgenic lines overexpressing OsTPS1, trehalose and proline concentrations were higher than in the wild type and some stress-related genes were up-regulated, including WSI18, RAB16C, HSP70, and ELIP. These results demonstrate that OsTPS1 may enhance the abiotic stress tolerance of plants by increasing the amount of trehalose and proline, and regulating the expression of stress-related genes. Furthermore, we found that overexpression of some Class II TPSs also enhanced plant tolerance of abiotic stress. This work will help to clarify the role of trehalose metabolism in abiotic stress response in higher plants.
authors.affiliation: Key Laboratory of Gene Engineering Drug and Biotechnology, College of Life Sciences, Beijing Normal University, Beijing, 100875, People's Republic of China.
authors.forename: Hao-Wen
authors.initials: HW
authors.lastname: Li
authors.forename: Bai-Sheng
authors.initials: BS
authors.lastname: Zang
authors.forename: Xing-Wang
authors.initials: XW
authors.lastname: Deng
authors.forename: Xi-Ping
authors.initials: XP
authors.lastname: Wang
mesh_terms: D000222:Adaptation, Physiological; D003080:Cold Temperature; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005816:Genetic Complementation Test; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D005964:Glucosyltransferases / Q000235:genetics / Q000378:metabolism*; D012275:Oryza / Q000201:enzymology / Q000235:genetics* / Q000502:physiology; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D011392:Proline / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D055051:Salt-Tolerant Plants / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D013312:Stress, Physiological; D014170:Transformation, Genetic; D014199:Trehalose / Q000378:metabolism
chemical_list: D011392:Proline; D014199:Trehalose; D005964:Glucosyltransferases; C023180:trehalose-6-phosphate synthase