title: Assessment of the application of an automated electronic milk analyzer for the enumeration of total bacteria in raw goat milk.
abstract: Automated electronic milk analyzers for rapid enumeration of total bacteria counts (TBC) are widely used for raw milk testing by many analytical laboratories worldwide. In Ontario, Canada, Bactoscan flow cytometry (BsnFC; Foss Electric, Hillerød, Denmark) is the official anchor method for TBC in raw cow milk. Penalties are levied at the BsnFC equivalent level of 50,000 cfu/mL, the standard plate count (SPC) regulatory limit. This study was conducted to assess the BsnFC for TBC in raw goat milk, to determine the mathematical relationship between the SPC and BsnFC methods, and to identify probable reasons for the difference in the SPC:BsnFC equivalents for goat and cow milks. Test procedures were conducted according to International Dairy Federation Bulletin guidelines. Approximately 115 farm bulk tank milk samples per month were tested for inhibitor residues, SPC, BsnFC, psychrotrophic bacteria count, composition (fat, protein, lactose, lactose and other solids, and freezing point), and somatic cell count from March 2009 to February 2010. Data analysis of the results for the samples tested indicated that the BsnFC method would be a good alternative to the SPC method, providing accurate and more precise results with a faster turnaround time. Although a linear regression model showed good correlation and prediction, tests for linearity indicated that the relationship was linear only beyond log 4.1 SPC. The logistic growth curve best modeled the relationship between the SPC and BsnFC for the entire sample population. The BsnFC equivalent to the SPC 50,000 cfu/mL regulatory limit was estimated to be 321,000 individual bacteria count (ibc)/mL. This estimate differs considerably from the BsnFC equivalent for cow milk (121,000 ibc/mL). Because of the low frequency of bulk tank milk pickups at goat farms, 78.5% of the samples had their oldest milking in the tank to be 6.5 to 9.0 d old when tested, compared with the cow milk samples, which had their oldest milking at 4 d old when tested. This may be one of the major factors contributing to the larger goat milk BsnFC equivalence. Correlations and interactions between various test results were also discussed to further understand differences between the 2 methods for goat and cow milks.
authors.affiliation: Laboratory Services Division, University of Guelph, Guelph N1H 8J7, Ontario, Canada. lramsaho@uoguelph.ca
authors.forename: L
authors.initials: L
authors.lastname: Ramsahoi
authors.forename: A
authors.initials: A
authors.lastname: Gao
authors.forename: M
authors.initials: M
authors.lastname: Fabri
authors.forename: J A
authors.initials: JA
authors.lastname: Odumeru
mesh_terms: D000818:Animals; D058491:Bacterial Load / Q000295:instrumentation / Q000379:methods / Q000662:veterinary*; D003612:Dairying / Q000295:instrumentation*; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods / Q000662:veterinary*; D006041:Goats; D008892:Milk / Q000382:microbiology* / Q000592:standards; D015203:Reproducibility of Results