title: Characterization of a CENP-B homolog in the holocentric Lepidoptera Spodoptera frugiperda.
abstract: The discovery of an homolog of the human centromeric protein B, CENP-B, in an EST database of the holocentric insect species Spodoptera frugiperda prompted us to further characterize that gene because i) CENP-B has not been described in invertebrates yet ii) it should be a milestone in the molecular characterization of the holocentric centromere of Lepidoptera. Like its human counterpart, the Sf CENP-B protein is related to the transposase of the pogo transposable element (TE) of D. melanogaster. In this paper, we show evidences that the lepidopteran cenpB gene has evolved from domestication of a transposase. Furthermore, the Sf CENP-B nuclear location and its ability to bind to a retrotransposon derived sequence in vivo argue in favor of a functional homology to CENP-B proteins.
authors.affiliation: UMR1333 INRA, Université Montpellier II, Place Eugène Bataillon, 34095 Montpellier Cedex 5, France. alencon@supagro.inra.fr
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: d'Alençon
authors.forename: Nicolas
authors.initials: N
authors.lastname: Nègre
authors.forename: Slavica
authors.initials: S
authors.lastname: Stanojcic
authors.forename: Benjamin
authors.initials: B
authors.lastname: Alassoeur
authors.forename: Sylvie
authors.initials: S
authors.lastname: Gimenez
authors.forename: Alexandre
authors.initials: A
authors.lastname: Léger
authors.forename: Adly
authors.initials: A
authors.lastname: Abd-Alla
authors.forename: Sylvie
authors.initials: S
authors.lastname: Juliant
authors.forename: Philippe
authors.initials: P
authors.lastname: Fournier
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002503:Centromere / Q000235:genetics; D051936:Centromere Protein B / Q000235:genetics* / Q000378:metabolism; D047369:Chromatin Immunoprecipitation; D003001:Cloning, Molecular; D003062:Codon; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation; D005260:Female; D049750:Genome, Insect; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D010957:Plasmids; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D018626:Retroelements / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D018411:Spodoptera / Q000235:genetics*; D019895:Transposases / Q000235:genetics / Q000378:metabolism
chemical_list: D051936:Centromere Protein B; D003062:Codon; D004268:DNA-Binding Proteins; D019476:Insect Proteins; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D018626:Retroelements; D019895:Transposases