title: Novel recombinant insulin analogue with flexible C-terminus in B chain. NMR structure of biosynthetic engineered A22G-B31K-B32R human insulin monomer in water/acetonitrile solution.
abstract: A tertiary structure of recombinant A22(G)-B31(K)-B32(R)-human insulin monomer (insulin GKR) has been characterized by (1)H, (13)C NMR at natural isotopic abundance using NOESY, TOCSY, (1)H/(13)C-GHSQC, and (1)H/(13)C-GHSQC-TOCSY spectra. Translational diffusion studies indicate the monomer structure in water/acetonitrile (65/35vol.%). CSI analysis confirms existence of secondary structure motifs present in human insulin standard (HIS). Both techniques allow to establish that in this solvent recombinant insulin GKR exists as a monomer. Starting from structures calculated by the program CYANA, two different refinement protocols used molecular dynamics simulated annealing with the program AMBER; in vacuum (AMBER_VC), and including a generalized Born solvent model (AMBER_GB). From these calculations an ensemble of 20 structures of lowest energy was chosen which represents the tertiary structure of studied insulin. Here we present novel insulin with added A22(G) amino acid which interacts with β-turn environment resulting in high flexibility of B chain C-terminus.
authors.affiliation: Institute of Biotechnology and Antibiotics, Warsaw, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Borowicz
authors.forename: Wojciech
authors.initials: W
authors.lastname: Bocian
authors.forename: Jerzy
authors.initials: J
authors.lastname: Sitkowski
authors.forename: Elżbieta
authors.initials: E
authors.lastname: Bednarek
authors.forename: Diana
authors.initials: D
authors.lastname: Mikiewicz-Syguła
authors.forename: Sylwia
authors.initials: S
authors.lastname: Błażej-Sosnowska
authors.forename: Monika
authors.initials: M
authors.lastname: Bogiel
authors.forename: Dorota
authors.initials: D
authors.lastname: Rusek
authors.forename: Dariusz
authors.initials: D
authors.lastname: Kurzynoga
authors.forename: Lech
authors.initials: L
authors.lastname: Kozerski
mesh_terms: D000097:Acetonitriles / Q000737:chemistry*; D020816:Amino Acid Motifs; D019943:Amino Acid Substitution; D004058:Diffusion; D006801:Humans; D061386:Insulin, Regular, Human / Q000031:analogs & derivatives* / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D015202:Protein Engineering; D020836:Protein Structure, Quaternary; D017434:Protein Structure, Tertiary; D012015:Reference Standards; D012996:Solutions; D014867:Water / Q000737:chemistry*
chemical_list: D000097:Acetonitriles; D061386:Insulin, Regular, Human; D012996:Solutions; D014867:Water; C032159:acetonitrile