title: Viruses with more than 1,000 genes: Mamavirus, a new Acanthamoeba polyphaga mimivirus strain, and reannotation of Mimivirus genes.
abstract: The genome sequence of the Mamavirus, a new Acanthamoeba polyphaga mimivirus strain, is reported. With 1,191,693 nt in length and 1,023 predicted protein-coding genes, the Mamavirus has the largest genome among the known viruses. The genomes of the Mamavirus and the previously described Mimivirus are highly similar in both the protein-coding genes and the intergenic regions. However, the Mamavirus contains an extra 5'-terminal segment that encompasses primarily disrupted duplicates of genes present elsewhere in the genome. The Mamavirus also has several unique genes including a small regulatory polyA polymerase subunit that is shared with poxviruses. Detailed analysis of the protein sequences of the two Mimiviruses led to a substantial amendment of the functional annotation of the viral genomes.
authors.affiliation: Unité de Recherche sur les Maladies Infectieuses et Tropicales Émergentes (URMITE), Centre National de la Recherche Scientifique UMR IRD 6236, Faculté de Médecine, Université de la Méditerranée, Marseille, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Colson
authors.forename: Natalya
authors.initials: N
authors.lastname: Yutin
authors.forename: Svetlana A
authors.initials: SA
authors.lastname: Shabalina
authors.forename: Catherine
authors.initials: C
authors.lastname: Robert
authors.forename: Ghislain
authors.initials: G
authors.lastname: Fournous
authors.forename: Bernard
authors.initials: B
authors.lastname: La Scola
authors.forename: Didier
authors.initials: D
authors.lastname: Raoult
authors.forename: Eugene V
authors.initials: EV
authors.lastname: Koonin
mesh_terms: D000048:Acanthamoeba / Q000821:virology*; D000595:Amino Acid Sequence; D000818:Animals; D004267:DNA Viruses / Q000235:genetics*; D016679:Genome, Viral / Q000235:genetics*; D056686:Mimiviridae / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D011062:Polynucleotide Adenylyltransferase / Q000145:classification / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014764:Viral Proteins / Q000145:classification / Q000235:genetics*
chemical_list: D014764:Viral Proteins; D011062:Polynucleotide Adenylyltransferase