title: Identification of the novel bioactive peptides dRYamide-1 and dRYamide-2, ligands for a neuropeptide Y-like receptor in Drosophila.
abstract: A number of bioactive peptides are involved in regulating a wide range of animal behaviors, including food consumption. Vertebrate neuropeptide Y (NPY) is a potent stimulator of appetitive behavior. Recently, Drosophila neuropeptide F (dNPF) and short NPF (sNPF), the Drosophila homologs of the vertebrate NPY, were identified to characterize the functions of NPFs in the feeding behaviors of this insect. Dm-NPFR1 and NPFR76F are the receptors for dNPF and sNPF, respectively; both receptors are G protein-coupled receptors (GPCRs). Another GPCR (CG5811; NepYR) was indentified in Drosophila as a neuropeptide Y-like receptor. Here, we identified 2 ligands of CG5811, dRYamide-1 and dRYamide-2. Both peptides are derived from the same precursor (CG40733) and have no significant structural similarities to known bioactive peptides. The C-terminal sequence RYamide of dRYamides is identical to that of NPY family peptides; on the other hand, dNPF and sNPF have C-terminal RFamide. When administered to blowflies, dRYamide-1 suppressed feeding motivation. We propose that dRYamides are related to the NPY family in vertebrates, similar to dNPF and sNPF.
authors.affiliation: Interdisciplinary Research Organization, University of Miyazaki, Miyazaki 889-1692, Japan. a0d203u@cc.miyazaki-u.ac.jp
authors.forename: Takanori
authors.initials: T
authors.lastname: Ida
authors.forename: Tomoko
authors.initials: T
authors.lastname: Takahashi
authors.forename: Hatsumi
authors.initials: H
authors.lastname: Tominaga
authors.forename: Takahiro
authors.initials: T
authors.lastname: Sato
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Kume
authors.forename: Mamiko
authors.initials: M
authors.lastname: Ozaki
authors.forename: Tetsutaro
authors.initials: T
authors.lastname: Hiraguchi
authors.forename: Toru
authors.initials: T
authors.lastname: Maeda
authors.forename: Hajime
authors.initials: H
authors.lastname: Shiotani
authors.forename: Saki
authors.initials: S
authors.lastname: Terajima
authors.forename: Hiroko
authors.initials: H
authors.lastname: Sano
authors.forename: Kenji
authors.initials: K
authors.lastname: Mori
authors.forename: Morikatsu
authors.initials: M
authors.lastname: Yoshida
authors.forename: Mikiya
authors.initials: M
authors.lastname: Miyazato
authors.forename: Johji
authors.initials: J
authors.lastname: Kato
authors.forename: Noboru
authors.initials: N
authors.lastname: Murakami
authors.forename: Kenji
authors.initials: K
authors.lastname: Kangawa
authors.forename: Masayasu
authors.initials: M
authors.lastname: Kojima
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D005247:Feeding Behavior; D008024:Ligands; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D017476:Receptors, Neuropeptide Y / Q000235:genetics / Q000378:metabolism*
chemical_list: D029721:Drosophila Proteins; D008024:Ligands; D009479:Neuropeptides; C561245:RYa protein, Drosophila; C561244:RYa-R protein, Drosophila; D017476:Receptors, Neuropeptide Y