title: Reproducible enrichment of extracellular heat shock proteins from blood serum using monomeric avidin.
abstract: Extracellular heat shock proteins (eHsps) in blood circulation have been associated with various diseases, including cancer. However, the lack of methods to enrich eHsps from serum samples has hampered the characterization of eHsps. This Letter presents our serendipitous finding that the monomeric avidin resin can serve as an affinity resin to enrich eHsps from blood serum. Biochemical mechanism of this eHsp enrichment as well as implications in biomarker discovery is discussed.
authors.affiliation: Department of Chemistry, Hunter College of CUNY, 695 Park Avenue, New York, NY 10065, USA.
authors.forename: Doina M
authors.initials: DM
authors.lastname: Mihai
authors.forename: Haiteng
authors.initials: H
authors.lastname: Deng
authors.forename: Akira
authors.initials: A
authors.lastname: Kawamura
mesh_terms: D001360:Avidin / Q000737:chemistry*; D015415:Biomarkers / Q000097:blood; D002846:Chromatography, Affinity; D006360:Heat-Shock Proteins / Q000097:blood / Q000302:isolation & purification*; D006801:Humans; D019169:Jurkat Cells; D009369:Neoplasms / Q000378:metabolism
chemical_list: D015415:Biomarkers; D006360:Heat-Shock Proteins; D001360:Avidin