title: Liquid chromatography-electrospray ionization tandem mass spectrometry for the quantification of styraxlignolide A in rat plasma.
abstract: Styraxlignolide A is a pharmacologically active ingredient isolated from Styrax japonica Sieb. et Zucc. A rapid, selective, and sensitive liquid chromatographic method with electrospray ionization tandem mass spectrometry was developed for use in the quantification of styraxlignolide A in rat plasma. Styraxlignolide A was extracted from rat plasma using ethyl acetate at neutral pH. The analytes were separated on an Atlantis dC18 column using a mixture of methanol and ammonium formate (10 mM, pH 3.0) (70:30, v/v) and detected by tandem mass spectrometry in multiple reaction monitoring mode. The standard curve was linear (r(2) =0.9978) over the concentration range of 100-10000 ng/mL. The lower limit of quantification was 100 ng/mL using 50 μL of plasma sample. The coefficient of variation and relative error for intra- and inter-assays at four QC levels were 1.6-8.3% and from -12.0 to -1.7%, respectively. The present method was applied successfully to the pharmacokinetic study of styraxlignolide A after intravenous administration of styraxlignolide A at a dose of 10 mg/kg in male Sprague-Dawley rats.
authors.affiliation: Drug Metabolism and Bioanalysis Laboratory, College of Pharmacy, The Catholic University of Korea, Bucheon, Korea.
authors.forename: Hye Y
authors.initials: HY
authors.lastname: Ji
authors.forename: Sei-Ryang
authors.initials: SR
authors.lastname: Oh
authors.forename: Hyeong-Kyu
authors.initials: HK
authors.lastname: Lee
authors.forename: Dong H
authors.initials: DH
authors.lastname: Na
authors.forename: Hye S
authors.initials: HS
authors.lastname: Lee
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid; D004187:Disaccharides / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry; D017705:Lignans / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry; D008297:Male; D015394:Molecular Structure; D051381:Rats; D017207:Rats, Sprague-Dawley; D021241:Spectrometry, Mass, Electrospray Ionization; D029644:Styracaceae / Q000737:chemistry*; D053719:Tandem Mass Spectrometry
chemical_list: D004187:Disaccharides; D017705:Lignans; C000599169:styraxlignolide A