title: Majority of CD4+ T cells from peripheral blood of HIV-1-infected individuals contain only one HIV DNA molecule.
abstract: Neither the number of HIV-1 proviruses within individual infected cells in HIV-1-infected patients nor their genetic relatedness within individual infected cells and between cells and plasma virus are well defined. To address these issues we developed a technique to quantify and genetically characterize HIV-1 DNA from single infected cells in vivo. Analysis of peripheral blood CD4(+) T cells from nine patients revealed that the majority of infected cells contain only one copy of HIV-1 DNA, implying a limited potential for recombination in virus produced by these cells. The genetic similarity between HIV populations in CD4(+) T cells and plasma implies ongoing exchange between these compartments both early and late after infection.
authors.affiliation: Department of Diagnostics and Vaccinology, Swedish Institute for Infectious Disease Control, 171 82, Solna, Sweden. Lina.Josefsson@smi.se
authors.forename: Lina
authors.initials: L
authors.lastname: Josefsson
authors.forename: Martin S
authors.initials: MS
authors.lastname: King
authors.forename: Barbro
authors.initials: B
authors.lastname: Makitalo
authors.forename: Johan
authors.initials: J
authors.lastname: Brännström
authors.forename: Wei
authors.initials: W
authors.lastname: Shao
authors.forename: Frank
authors.initials: F
authors.lastname: Maldarelli
authors.forename: Mary F
authors.initials: MF
authors.lastname: Kearney
authors.forename: Wei-Shau
authors.initials: WS
authors.lastname: Hu
authors.forename: Jianbo
authors.initials: J
authors.lastname: Chen
authors.forename: Hans
authors.initials: H
authors.lastname: Gaines
authors.forename: John W
authors.initials: JW
authors.lastname: Mellors
authors.forename: Jan
authors.initials: J
authors.lastname: Albert
authors.forename: John M
authors.initials: JM
authors.lastname: Coffin
authors.forename: Sarah E
authors.initials: SE
authors.lastname: Palmer
mesh_terms: D001483:Base Sequence; D015496:CD4-Positive T-Lymphocytes / Q000821:virology*; D002908:Chronic Disease; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000097:blood* / Q000235:genetics*; D014644:Genetic Variation; D015658:HIV Infections / Q000097:blood* / Q000821:virology*; D015497:HIV-1 / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D010802:Phylogeny; D011533:Proviruses / Q000235:genetics / Q000302:isolation & purification; D012367:RNA, Viral / Q000097:blood / Q000235:genetics; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA / Q000379:methods; D013997:Time Factors; D019562:Viral Load
chemical_list: D017931:DNA Primers; D004279:DNA, Viral; D012367:RNA, Viral