title: Development of a multiplex amplification refractory mutation system for simultaneous authentication of Korean ginseng cultivars "Gumpoong" and "Chungsun".
abstract: BACKGROUND AND AIMS. Molecular authentication of Korean ginseng cultivars was investigated using the mitochondrial nicotinamide adenine dinucleotide (NADH) dehydrogenase subunit 7 (nad7) intron 3 region. MATERIALS AND METHODS. A mutation site specific to Panax ginseng "Gumpoong" and "Chungsun" cultivars was detected within the sequence data. Based on this mutation site and the "Gumpoong"-specific single nucleotide polymorphism site reported in 26S rDNA, two modified allele-specific primer pairs were designed and a multiplex amplification refractory mutation system (MARMS) was applied to identify "Gumpoong" and "Chungsun." RESULTS. The results showed that "Gumpoong" and "Chungsun" can be clearly discriminated from the other Korean ginseng cultivars by simultaneously identifying the haplotype of "Gumpoong" and the specific allele of "Chungsun" by applying the MARMS. CONCLUSION. This study, therefore, provides a simple and reliable method for simultaneous authentication of "Gumpoong" and "Chungsun" cultivars.
authors.affiliation: Korean Ginseng Center for Most Valuable Products & Ginseng Genetic Resource Bank, Kyung Hee University, Yongin, Gyunggi-do, Republic of Korea.
authors.forename: Hongtao
authors.initials: H
authors.lastname: Wang
authors.forename: Woo-Saeng
authors.initials: WS
authors.lastname: Kwon
authors.forename: Dong-Uk
authors.initials: DU
authors.lastname: Yang
authors.forename: Min-Kyeoung
authors.initials: MK
authors.lastname: Kim
authors.forename: Subramaniyam
authors.initials: S
authors.lastname: Sathiyamoorthy
authors.forename: Haizhu
authors.initials: H
authors.lastname: Jin
authors.forename: Deok-Chun
authors.initials: DC
authors.lastname: Yang
mesh_terms: D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D006239:Haplotypes / Q000235:genetics; D008969:Molecular Sequence Data; D009245:NADH Dehydrogenase / Q000235:genetics*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D005894:Panax / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D021122:Protein Subunits / Q000235:genetics; D056910:Republic of Korea; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D021122:Protein Subunits; D009245:NADH Dehydrogenase