title: Influence of HLA-DQ2 and DQ8 on severity in celiac Disease.
abstract: BACKGROUND
  HLA-DQB1*02 homozygosity was shown to be more common in patients with complicated rather than uncomplicated celiac disease (CD).
  
  
  OBJECTIVE
  To study HLA-DQA1 and DQB1 profile in adult patients with different forms of CD, including patients with complicated and potential CD, the most affected and the most preserved histologic end of the pathologic celiac spectrum.
  
  
  METHODS
  HLA-DQA1 and DQB1 molecular typing was performed in 218 adult CD patients (169 with uncomplicated CD, 27 with complicated CD, and 22 with potential CD) and 224 healthy stem cell donors. HLA-DQA1 and DQB1 gene polymorphism was analyzed using polymerase chain reaction sequence-specific primers and/or reverse polymerase chain reaction sequence-specific oligonucleotides techniques.
  
  
  RESULTS
  As expected, the frequency of HLA-DQB1*02 allele, DQB1*02 homozygosity, and DQB1*0302 gene were statistically different in the 4 groups. However, multivariate analysis demonstrated that patients with potential CD have a higher frequency of both HLA-DQB1*0302 and HLA-DQB1*0603 alleles and a reduced frequency of DQB1*02 homozygosity compared with patients with uncomplicated and complicated CD.
  
  
  CONCLUSIONS
  The increased frequency of DQB1*0302 and the reduced frequency of DQB1*02 homozygosity in potential CD is consistent with the idea that different clinical/pathologic evolutions might be related to different immunogeneses. This could be clinically relevant in the future.
authors.affiliation: Coeliac Centre/First Department of Internal Medicine, Fondazione IRCCS Policlinico San Matteo, University of Pavia, Italy. f.biagi@smatteo.pv.it
authors.forename: Federico
authors.initials: F
authors.lastname: Biagi
authors.forename: Paola I
authors.initials: PI
authors.lastname: Bianchi
authors.forename: Claudia
authors.initials: C
authors.lastname: Vattiato
authors.forename: Alessandra
authors.initials: A
authors.lastname: Marchese
authors.forename: Lucia
authors.initials: L
authors.lastname: Trotta
authors.forename: Carla
authors.initials: C
authors.lastname: Badulli
authors.forename: Annalisa
authors.initials: A
authors.lastname: De Silvestri
authors.forename: Miryam
authors.initials: M
authors.lastname: Martinetti
authors.forename: Gino R
authors.initials: GR
authors.lastname: Corazza
mesh_terms: D000293:Adolescent; D000328:Adult; D000483:Alleles; D002446:Celiac Disease / Q000235:genetics* / Q000503:physiopathology; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006683:HLA-DQ Antigens / Q000235:genetics; D059848:HLA-DQ alpha-Chains / Q000235:genetics*; D059866:HLA-DQ beta-Chains / Q000235:genetics*; D006720:Homozygote; D006801:Humans; D008297:Male; D008875:Middle Aged; D058889:Molecular Typing; D015999:Multivariate Analysis; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D012189:Retrospective Studies; D012720:Severity of Illness Index; D055815:Young Adult
chemical_list: D006683:HLA-DQ Antigens; D059848:HLA-DQ alpha-Chains; D059866:HLA-DQ beta-Chains; C053186:HLA-DQ2 antigen; C109756:HLA-DQ8 antigen; C069050:HLA-DQA1 antigen; C069051:HLA-DQB1 antigen