title: In silico designing of insecticidal small interfering RNA (siRNA) for Helicoverpa armigera control.
abstract: Helicoverpa armigera, a polyphagous lepidopteron insect pest causes severe yield loss in cotton, legumes, tomato, okra and other crops. Application of chemical pesticides although effective, has human health and environmental safety concerns. Moreover, development of resistance against most of the available pesticides is compelling to look for alternative strategies. Adoption of Bt transgenic crops have resulted in reduction in pesticide consumption and increasing crop productivity. However, sustainability of Bt transgenic crops is threatened by the emergence of insect resistance. In the present study potential insecticidal siRNA were identified in six H. armigera horrhonal pathway genes. Out of over 2000 computationally identified siRNA, 16 most promising siRNA were selected that address the biosafety concerns and have high potential of targeted gene silencing. These siRNA will be useful for chemical synthesis, in insect feeding assays and knockdown the target H. armigera hormone biosynthesis, consequently obstructing the completion of insect life cycle. The siRNA have a great potential of deployment to control H. annrmigera alone as well as with Bt for insect resistance management.
authors.affiliation: National Institute of Malaria Research, 22 Sham Nath Marg, Delhi 110 054, India.
authors.forename: Meenakshi
authors.initials: M
authors.lastname: Choudhary
authors.forename: Shakti
authors.initials: S
authors.lastname: Sahi
mesh_terms: D000818:Animals; D001483:Base Sequence; D020868:Gene Silencing; D017344:Genes, Insect; D007300:Insect Control / Q000379:methods*; D007301:Insect Hormones / Q000235:genetics; D007915:Lepidoptera / Q000235:genetics* / Q000472:pathogenicity*; D034741:RNA, Small Interfering / Q000235:genetics*
chemical_list: D007301:Insect Hormones; D034741:RNA, Small Interfering