title: [Change of IL-22R1 expression in human airway smooth muscle cells in response to different stimulating agents].
abstract: OBJECTIVE
  To explore the effects of serum of asthmatic patients, dexamethasone, interleukin-4 (IL-4), interferon-gamma (IFN-γ) and transforming growth factor-β (TGF-β) on the expression of interleukin-22 receptor 1 (IL-22R1) mRNA and protein in HASMCs in vitro.
  
  
  METHODS
  IL-22R1 mRNA and protein expressions in HASMCs treated with different stimulating agents were measured by real-time PCR and Western blotting, respectively.
  
  
  RESULTS
  IL-22R1 mRNA and protein expressions in HASMCs were significantly increased after stimulation by serum from asthmatic patients, but decreased after co-stimulation with dexamethasone. IL-22R1 mRNA and protein expressions in the cells both increased after stimulation by IL-4, IFN-γ and TGF-β.
  
  
  CONCLUSIONS
  IL-22R1 in HASMCs might be involved in the pathogenesis of asthma, and the therapeutic effect of dexamethasone on asthma is mediated, at least partially, by IL-22R1. The effects of IFN-γ, IL-4, and TGF-β on asthma may also be attributed to their actions on HASMCs.
authors.affiliation: Department of Respiratory Disease, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. nlmc@163.com
authors.forename: Zhan
authors.initials: Z
authors.lastname: Zhang
authors.forename: Ya-ling
authors.initials: YL
authors.lastname: Luo
authors.forename: Li-li
authors.initials: LL
authors.lastname: Zhou
authors.forename: Wen-yan
authors.initials: WY
authors.lastname: Lai
authors.forename: Jian
authors.initials: J
authors.lastname: Xu
authors.forename: Zhen-xing
authors.initials: ZX
authors.lastname: Li
authors.forename: Dun-qiang
authors.initials: DQ
authors.lastname: Ren
authors.forename: Han
authors.initials: H
authors.lastname: Ye
authors.forename: Hao-hai
authors.initials: HH
authors.lastname: Zhong
mesh_terms: D001249:Asthma / Q000097:blood; D002460:Cell Line; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D015847:Interleukin-4 / Q000494:pharmacology; D032389:Myocytes, Smooth Muscle / Q000187:drug effects* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D018123:Receptors, Interleukin / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000494:pharmacology
chemical_list: D012333:RNA, Messenger; D018123:Receptors, Interleukin; D016212:Transforming Growth Factor beta; C415369:interleukin-22 receptor; D015847:Interleukin-4; D007371:Interferon-gamma