title: In vitro antitumor activity of broccolini seeds extracts.
abstract: Broccolini (Brassica oleracea Italica × Alboglabra) is a hybrid of broccoli and kai-lan, Chinese broccoli. To date, no report on antitumor activity of Broccolini (NOT Broccoli) is available. In this study, we evaluated the antiproliferative effects of broccolini seeds extract (BSE) on human lung and ovarian cancer cells. It was found that BSE induces A549 and OVCAR-3 cells apoptosis in a dose-dependent manner by using MTT assay. The IC(50) values of BSE in A549 and OVCAR-3 cells were estimated to be 81.94 and 78.6 µg/ml, respectively. Furthermore, the phase contrast microscope showed that in high-dose group (90∼120 µg/ml), the morphology structure of OVCAR-3 cells become irregular and exhibited characteristics of apoptosis such as cell membrane shrinkage, condensation and fragmentation of nuclear chromatin as well as formation of apoptotic bodies.
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, China.
authors.forename: Yanjing
authors.initials: Y
authors.lastname: Yang
authors.forename: Xuewu
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis; D001937:Brassica / Q000737:chemistry*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects; D006801:Humans; D020128:Inhibitory Concentration 50; D008858:Microscopy, Phase-Contrast; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry*; D013778:Tetrazolium Salts / Q000378:metabolism; D013844:Thiazoles / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D010936:Plant Extracts; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue