title: A high throughput method and culture medium for rapid screening of phosphate accumulating microorganisms.
abstract: A novel PA Medium (PAM) for efficient screening of phosphate-accumulating organisms (PAOs) was developed taking Serratia marcescens NBRI1213 as model organism. The defined National Botanical Research Institute's growth medium (NBRI) supplemented with 0.1% maltose, designed for quantitative estimation of phosphate accumulation was designated as PAM. Our work suggested usage of PAM for efficient qualitative screening and as a microbiological medium for preferential selection of PAOs on Petri-plates. For qualitative screening of PAOs, Toluidine blue-O dye (TBO) was supplemented in PAM, designated as PAM-TBO. Qualitative analysis of phosphate accumulated by various groups correlated well with grouping based upon quantitative analysis of PAOs, effect of carbon, nitrogen, salts, and phosphate accumulation-defective transposon mutants. For significantly increasing sample throughput, efficiency of screening PAOs was further enhanced by adaptation of PAM-TBO assay to microtiter plate based method. It is envisaged that usage of this medium will be salutary for quick screening of PAOs from environment.
authors.affiliation: Division of Plant Microbe Interactions, National Botanical Research Institute, Rana Pratap Marg, Lucknow 226 001, India.
authors.forename: Vasvi
authors.initials: V
authors.lastname: Chaudhry
authors.forename: Chandra Shekhar
authors.initials: CS
authors.lastname: Nautiyal
mesh_terms: D002244:Carbon / Q000378:metabolism; D003470:Culture Media; D016296:Mutagenesis; D009584:Nitrogen / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism*; D012706:Serratia marcescens / Q000378:metabolism*
chemical_list: D003470:Culture Media; D010710:Phosphates; D002244:Carbon; D009584:Nitrogen