title: High-throughput measurement and classification of organic P in environmental samples.
abstract: Many types of organic phosphorus (P) molecules exist in environmental samples. Traditional P measurements do not detect these organic P compounds since they do not react with colorimetric reagents. Enzymatic hydrolysis (EH) is an emerging method for accurately characterizing organic P forms in environmental samples. This method is only trumped in accuracy by Phosphorus-31 Nuclear Magnetic Resonance Spectroscopy ((31)P-NMR)--a method that is expensive and requires specialized technical training. We have adapted an enzymatic hydrolysis method capable of measuring three classes of phosphorus (monoester P, diester P and inorganic P) to a microplate reader system. This method provides researchers with a fast, accurate, affordable and user-friendly means to measure P species in soils, sediments, manures and, if concentrated, aquatic samples. This is the only high-throughput method for measuring the forms and enzyme-lability of organic P that can be performed in a standard laboratory. The resulting data provides insight to scientists studying system nutrient content and eutrophication potential.
authors.affiliation: School of Engineering, University of Vermont, USA.
authors.forename: Nicholas R
authors.initials: NR
authors.lastname: Johnson
authors.forename: Jane E
authors.initials: JE
authors.lastname: Hill
mesh_terms: D000135:Acid Phosphatase / Q000737:chemistry; D003124:Colorimetry / Q000379:methods*; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D009930:Organic Chemicals / Q000032:analysis* / Q000737:chemistry; D010758:Phosphorus / Q000032:analysis* / Q000737:chemistry; D012987:Soil / Q000032:analysis
chemical_list: D009930:Organic Chemicals; D012987:Soil; D010758:Phosphorus; D000135:Acid Phosphatase