title: A strategic and systematic approach for the determination of biosensor regeneration conditions.
abstract: Determining the optimal conditions for surface regeneration is fundamental for performance of efficient and robust protein-protein interaction kinetic studies. We devised a systematic methodology comprised of an automated seven-cycle analyte and buffer injection Biacore scheme and data interpretation algorithm. The efficiency and utility is illustrated using an antigen/monoclonal antibody interaction that required ultimately six pulses of acid for regeneration. This technique has broad applicability to any biosensor assay that requires regeneration of a surface.
authors.affiliation: Takeda San Francisco, Inc., 285 East Grand Avenue, South San Francisco, CA 94080, USA. adrake@takedasf.com
authors.forename: Andrew W
authors.initials: AW
authors.lastname: Drake
authors.forename: Scott L
authors.initials: SL
authors.lastname: Klakamp
mesh_terms: D000465:Algorithms; D000911:Antibodies, Monoclonal; D000937:Antigen-Antibody Reactions; D015374:Biosensing Techniques / Q000379:methods* / Q000706:statistics & numerical data; D002021:Buffers; D025941:Protein Interaction Mapping / Q000379:methods; D012985:Software Design; D013499:Surface Properties
chemical_list: D000911:Antibodies, Monoclonal; D002021:Buffers