title: No association between variant N-acetyltransferase genes, cigarette smoking and Prostate Cancer susceptibility among men of African descent.
abstract: OBJECTIVE: We evaluated the individual and combination effects of NAT1, NAT2 and tobacco smoking in a case-control study of 219 incident prostate cancer (PCa) cases and 555 disease-free men. METHODS: Allelic discriminations for 15 NAT1 and NAT2 loci were detected in germ-line DNA samples using TaqMan polymerase chain reaction (PCR) assays. Single gene, gene-gene and gene-smoking interactions were analyzed using logistic regression models and multi-factor dimensionality reduction (MDR) adjusted for age and subpopulation stratification. MDR involves a rigorous algorithm that has ample statistical power to assess and visualize gene-gene and gene-environment interactions using relatively small samples sizes (i.e., 200 cases and 200 controls). RESULTS: Despite the relatively high prevalence of NAT1*10/*10 (40.1%), NAT2 slow (30.6%), and NAT2 very slow acetylator genotypes (10.1%) among our study participants, these putative risk factors did not individually or jointly increase PCa risk among all subjects or a subset analysis restricted to tobacco smokers. CONCLUSION: Our data do not support the use of N-acetyltransferase genetic susceptibilities as PCa risk factors among men of African descent; however, subsequent studies in larger sample populations are needed to confirm this finding.
authors.affiliation: Department of Pharmacology & Toxicology, School of Medicine, University of Louisville (UofL), Louisville, KY, U.S.A.
authors.forename: La Creis Renee
authors.initials: LC
authors.lastname: Kidd
authors.forename: Tiva T
authors.initials: TT
authors.lastname: Vancleave
authors.forename: Mark A
authors.initials: MA
authors.lastname: Doll
authors.forename: Daya S
authors.initials: DS
authors.lastname: Srivastava
authors.forename: Brandon
authors.initials: B
authors.lastname: Thacker
authors.forename: Oyeyemi
authors.initials: O
authors.lastname: Komolafe
authors.forename: Vasyl
authors.initials: V
authors.lastname: Pihur
authors.forename: Guy N
authors.initials: GN
authors.lastname: Brock
authors.forename: David W
authors.initials: DW
authors.lastname: Hein