title: MultiFit: a web server for fitting multiple protein structures into their electron microscopy density map.
abstract: Advances in electron microscopy (EM) allow for structure determination of large biological assemblies at increasingly higher resolutions. A key step in this process is fitting multiple component structures into an EM-derived density map of their assembly. Here, we describe a web server for this task. The server takes as input a set of protein structures in the PDB format and an EM density map in the MRC format. The output is an ensemble of models ranked by their quality of fit to the density map. The models can be viewed online or downloaded from the website. The service is available at; http://salilab.org/multifit/ and http://bioinfo3d.cs.tau.ac.il/.
authors.affiliation: Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, CA, USA.
authors.forename: Elina
authors.initials: E
authors.lastname: Tjioe
authors.forename: Keren
authors.initials: K
authors.lastname: Lasker
authors.forename: Ben
authors.initials: B
authors.lastname: Webb
authors.forename: Haim J
authors.initials: HJ
authors.lastname: Wolfson
authors.forename: Andrej
authors.initials: A
authors.lastname: Sali
mesh_terms: D020407:Internet; D008854:Microscopy, Electron / Q000379:methods*; D008958:Models, Molecular; D046912:Multiprotein Complexes / Q000737:chemistry / Q000648:ultrastructure*; D012984:Software
chemical_list: D046912:Multiprotein Complexes