title: Sensing amyloid-β aggregation using luminescent dipyridophenazine ruthenium(II) complexes.
abstract: The aggregation of amyloid-β (Aβ) peptides has been associated with the onset of Alzheimer's disease. Here, we report the use of a luminescent dipyridophenazine ruthenium(II) complex to monitor Aβ fibrillization. This complex is not photoluminescent in aqueous solution nor in the presence of monomeric Aβ, but it presents a strong photoluminescence in the presence of Aβ fibril aggregates. One of the advantages of this metal complex is its large Stokes shift (180 nm). Furthermore, the long-lived photoluminescence lifetime of this ruthenium complex allows its use for the detection of fibrillar proteins in the presence of short-lived fluorescent backgrounds, using time-gating technology. We will present evidence of the advantages of dipyridophenazine ruthenium(II) complexes for monitoring protein fibrillization in highly fluorescent media.
authors.affiliation: Department of Chemistry, Rice University, 6100 South Main Street, Houston, Texas 77005, USA.
authors.forename: Nathan P
authors.initials: NP
authors.lastname: Cook
authors.forename: Veronica
authors.initials: V
authors.lastname: Torres
authors.forename: Disha
authors.initials: D
authors.lastname: Jain
authors.forename: Angel A
authors.initials: AA
authors.lastname: Martí
mesh_terms: D000544:Alzheimer Disease / Q000175:diagnosis / Q000378:metabolism*; D016229:Amyloid beta-Peptides / Q000378:metabolism* / Q000648:ultrastructure; D006801:Humans; D049408:Luminescent Agents / Q000032:analysis* / Q000378:metabolism; D008163:Luminescent Measurements / Q000379:methods*; D009942:Organometallic Compounds / Q000032:analysis* / Q000378:metabolism
chemical_list: D016229:Amyloid beta-Peptides; D049408:Luminescent Agents; D009942:Organometallic Compounds; C483523:ruthenium dipyridophenazine