title: Detection of malarial byproduct hemozoin utilizing its unique scattering properties.
abstract: The scattering characteristics of the malaria byproduct hemozoin, including its scattering distribution and depolarization, are modeled using Discrete Dipole Approximation (DDA) and compared to those of healthy red blood cells. Scattering (or dark-field) spectroscopy and imaging are used to identify hemozoin in fresh rodent blood samples. A new detection method is proposed and demonstrated using dark-field in conjunction with cross-polarization imaging and spectroscopy. SNRs greater than 50:1 are achieved for hemozoin in fresh blood without the addition of stains or reagents. The potential of such a detection system is discussed.
authors.affiliation: Intellectual Ventures Laboratories, 1600 132nd Avenue, NE, Bellevue, WA 98005, USA. bwilson@intven.com
authors.forename: Benjamin K
authors.initials: BK
authors.lastname: Wilson
authors.forename: Matthew R
authors.initials: MR
authors.lastname: Behrend
authors.forename: Matthew P
authors.initials: MP
authors.lastname: Horning
authors.forename: Michael C
authors.initials: MC
authors.lastname: Hegg
mesh_terms: D000818:Animals; D004867:Equipment Design; D004912:Erythrocytes / Q000469:parasitology*; D006420:Hemeproteins / Q000032:analysis*; D008288:Malaria / Q000175:diagnosis* / Q000469:parasitology; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*; D010967:Plasmodium yoelii / Q000737:chemistry* / Q000302:isolation & purification; D012377:Rodentia; D012542:Scattering, Radiation
chemical_list: D006420:Hemeproteins; C020753:hemozoin