title: Influence of dietary iodine deficiency on the thyroid gland in Slc26a4-null mutant mice.
abstract: BACKGROUND
  Pendred syndrome (PDS) is an autosomal recessive disorder characterized by sensorineural hearing impairment and variable degree of goitrous enlargement of the thyroid gland with a partial defect in iodine organification. The thyroid function phenotype can range from normal function to overt hypothyroidism. It is caused by loss-of-function mutations in the SLC26A4 (PDS) gene. The severity of the goiter has been postulated to depend on the amount of dietary iodine intake. However, direct evidence has not been shown to support this hypothesis. Because Slc26a4-null mice have deafness but do not develop goiter, we fed the mutant mice a control diet or an iodine-deficient diet to evaluate whether iodine deficiency is a causative environmental factor for goiter development in PDS.
  
  
  METHODS
  We evaluated the thyroid volume in histological sections with the use of three-dimensional reconstitution software, we measured serum levels of total tri-iodothyronine (TT3) and total thyroxine (TT4) levels, and we studied the thyroid gland morphology by transmission electron microscopy.
  
  
  RESULTS
  TT4 levels became low but TT3 levels did not change significantly after eight weeks of an iodine-deficient diet compared to levels in the control diet animals. Even in Slc26a4-null mice fed an iodine-deficient diet, the volume of the thyroid gland did not increase although the size of each epithelial cell increased with a concomitant decrease of thyroid colloidal area.
  
  
  CONCLUSIONS
  An iodine-deficient diet did not induce goiter in Slc26a4-null mice, suggesting that other environmental, epigenetic or genetic factors are involved in goiter development in PDS.
authors.affiliation: Department of Otorhinolaryngology Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, Aichi 466-8550, Japan. tsutomun@med.nagoya-u.ac.jp.
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Iwata
authors.forename: Tadao
authors.initials: T
authors.lastname: Yoshida
authors.forename: Masaaki
authors.initials: M
authors.lastname: Teranishi
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Murata
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Hayashi
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Kanou
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Griffith
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Nakashima