title: Pre-absorbed immunoproteomics: a novel method for the detection of Streptococcus suis surface proteins.
abstract: Streptococcus suis serotype 2 (SS2) is a zoonotic pathogen that can cause infections in pigs and humans. Bacterial surface proteins are often investigated as potential vaccine candidates and biomarkers of virulence. In this study, a novel method for identifying bacterial surface proteins is presented, which combines immunoproteomic and immunoserologic techniques. Critical to the success of this new method is an improved procedure for generating two-dimensional electrophoresis gel profiles of S. suis proteins. The S. suis surface proteins identified in this study include muramidase-released protein precursor (MRP) and an ABC transporter protein, while MRP is thought to be one of the main virulence factors in SS2 located on the bacterial surface. Herein, we demonstrate that the ABC transporter protein can bind to HEp-2 cells, which strongly suggests that this protein is located on the bacterial cell surface and may be involved in pathogenesis. An immunofluorescence assay confirmed that the ABC transporter is localized to the bacterial outer surface. This new method may prove to be a useful tool for identifying surface proteins, and aid in the development of new vaccine subunits and disease diagnostics.
authors.affiliation: Key Laboratory of Animal Disease Diagnostic & Immunology, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Guangjin
authors.initials: G
authors.lastname: Liu
authors.forename: Fang
authors.initials: F
authors.lastname: Tang
authors.forename: Jing
authors.initials: J
authors.lastname: Shao
authors.forename: Yan
authors.initials: Y
authors.lastname: Lu
authors.forename: Yinli
authors.initials: Y
authors.lastname: Bao
authors.forename: Huochun
authors.initials: H
authors.lastname: Yao
authors.forename: Chengping
authors.initials: C
authors.lastname: Lu
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D002460:Cell Line; D019295:Computational Biology / Q000379:methods; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D006801:Humans; D007525:Isoelectric Focusing; D008565:Membrane Proteins / Q000235:genetics / Q000276:immunology*; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D017011:Streptococcus suis / Q000276:immunology*; D013552:Swine
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins