title: Molecular characterization of Der p 10: a diagnostic marker for broad sensitization in house dust mite allergy.
abstract: BACKGROUND
  Tropomyosins represent clinically relevant seafood allergens but the role of mite tropomyosin, Der p 10, in house dust mite (HDM) allergy has not been studied in detail.
  
  
  OBJECTIVE
  To express and purify a recombinant Der p 10 with equivalent IgE reactivity as natural Der p 10 and to evaluate its IgE reactivity and allergenic activity in HDM-allergic patients.
  
  
  METHODS
  rDer p 10 was expressed in Escherichia coli, purified and characterized by mass spectrometry and circular dichroism. It was tested for IgE reactivity in 1322 HDM-allergic patients. Detailed IgE-reactivity profiles to six HDM allergens (Der p 1, 2, 5, 7, 10, 21) were established for subgroups of Der p 10-positive and -negative patients. The allergenic activity of rDer p 10 was evaluated in basophil degranulation experiments.
  
  
  RESULTS
  rDer p 10 is an α-helical protein sharing IgE epitopes with nDer p 10. It is recognized by 15.2% of HDM-allergic patients. Der p 10-negative patients were primarily sensitized to Der p 1 and/or Der p 2, whereas Der p 10-positive patients reacted to several other HDM allergens besides the major allergens (Der p 1, Der p 2) or showed a rather selective Der p 10 reactivity. The allergenic activity of Der p 10 was generally low but patients could be identified who suffered from clinically relevant HDM allergy due to Der p 10 sensitization.
  
  
  CONCLUSIONS
  Der p 10 may be a diagnostic marker for HDM-allergic patients with additional sensitization to allergens other than Der p 1 and Der p 2. Such patients may require attention when allergen-specific immunotherapy is considered.
authors.affiliation: Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, AustriaAllergy Centre Vienna West, Vienna, Austria.
authors.forename: Y
authors.initials: Y
authors.lastname: Resch
authors.forename: M
authors.initials: M
authors.lastname: Weghofer
authors.forename: S
authors.initials: S
authors.lastname: Seiberler
authors.forename: F
authors.initials: F
authors.lastname: Horak
authors.forename: S
authors.initials: S
authors.lastname: Scheiblhofer
authors.forename: B
authors.initials: B
authors.lastname: Linhart
authors.forename: I
authors.initials: I
authors.lastname: Swoboda
authors.forename: W R
authors.initials: WR
authors.lastname: Thomas
authors.forename: J
authors.initials: J
authors.lastname: Thalhamer
authors.forename: R
authors.initials: R
authors.lastname: Valenta
authors.forename: S
authors.initials: S
authors.lastname: Vrtala
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D039741:Antigens, Dermatophagoides / Q000737:chemistry / Q000235:genetics*; D060829:Arthropod Proteins / Q000737:chemistry / Q000235:genetics*; D002648:Child; D002942:Circular Dichroism; D040002:Dermatophagoides pteronyssinus / Q000276:immunology / Q000378:metabolism; D003888:Desensitization, Immunologic / Q000379:methods; D004391:Dust / Q000276:immunology; D005260:Female; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000175:diagnosis* / Q000276:immunology; D007073:Immunoglobulin E / Q000097:blood; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D051381:Rats; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D014335:Tropomyosin / Q000737:chemistry / Q000235:genetics*; D055815:Young Adult
chemical_list: D039741:Antigens, Dermatophagoides; D060829:Arthropod Proteins; D004391:Dust; D011994:Recombinant Proteins; D014335:Tropomyosin; C111884:der p 10 protein, Dermatophagoides pteronyssinus; D007073:Immunoglobulin E