title: Association of EphA4 polymorphism with swine reproductive traits and mRNA expression of EphA4 during embryo implantation.
abstract: The swine erythropoietin-producing hepatocellular A4 (EphA4) gene, which was detected in the endometrium during embryo implantation in pigs, was one of the potential candidate genes for reproductive traits. In the study, two single nucleotide polymorphism (SNP) loci (EphA4_1 and EphA4_2) in exon 3 of EphA4 gene were analyzed to determine whether EphA4 influenced total number born (TNB) and number born alive (NBA). Association of two diallelic polymorphisms with reproductive traits was assessed in Landrace, Yorkshire and Duroc populations with 2,014 litter records of 765 sows. The results showed that G allele at EphA4_1 locus or C allele at EphA4_2 locus seemed to have advantageous effects on litter size. And the combined analyzed results demonstrated that genotype AGTC, AGCC and GGCC are better than genotype AATT, AATC and AGTT for TNB and NBA in either single parity or all parities. The results in this study demonstrated that EphA4 gene was significantly associated with litter size in pigs. In addition, a high mRNA expression of EphA4 was found in small intestine, large intestine, stomach and endometrium, and the expression decreased during implantation in pigs. Further studies were needed to confirm these preliminary researches.
authors.affiliation: College of Animal Science and Technology, China Agricultural University/The Key Laboratory of Animal Genetics and Breeding of Ministry of Agriculture, 100193 Beijing, People's Republic of China. 995852526@qq.com
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Fu
authors.forename: Jinluan
authors.initials: J
authors.lastname: Fu
authors.forename: Aiguo
authors.initials: A
authors.lastname: Wang
mesh_terms: D000818:Animals; D017931:DNA Primers / Q000235:genetics; D010064:Embryo Implantation / Q000235:genetics*; D004717:Endometrium / Q000378:metabolism; D005260:Female; D056726:Genetic Association Studies / Q000662:veterinary; D014644:Genetic Variation; D016014:Linear Models; D008098:Litter Size / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D018807:Polymorphism, Single-Stranded Conformational; D012333:RNA, Messenger / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D036122:Receptor, EphA4 / Q000235:genetics* / Q000378:metabolism; D012098:Reproduction / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D013552:Swine / Q000196:embryology / Q000235:genetics*
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger; D036122:Receptor, EphA4