title: [Contribution to the mobile phase selection for HPLC separation in order to make the identification and quantitative determination using the UV-VIS detector].
abstract: The aim of this study is to elaborate a HPLC with UV detection method for the analysis of some AINS, method which can be transferred in a system with mass spectrometry detection.
  
  
  METHODS
  More AINS drugs (diclofenac sodium, ibuprophen, ketoprofen, meloxicame, piroxicame, tenoxicame, nimesulide, phenylbutazone and indomethacin) were studied by UV spectrophotometry and by HPLC.
  
  
  RESULTS
  The UV spectra of the studied AINS were recorded in pure solvents or in a mixture of solvents in order to establish the absorbance that will be used for HPLC detection in UV. To establish the optimum chromatographic parameters, the influence of the flow and of the composition of the mobile phase (both of the aqueous and organic phases) were studied too.
  
  
  CONCLUSIONS
  The proposed procedure is easy and relatively with low cost regarding the MS detection.
authors.affiliation: Facultatea de Farmacie, Disciplina Chimie Analitică, Universitatea de Medicină si Farmacie Gr.T. Popa Iaşi.
authors.forename: J
authors.initials: J
authors.lastname: Negru
authors.forename: A F
authors.initials: AF
authors.lastname: Spac
authors.forename: V
authors.initials: V
authors.lastname: Dorneanu
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D012997:Solvents