title: Fabrication of porous chitosan/poly(vinyl alcohol) reinforced single-walled carbon nanotube nanocomposites for neural tissue engineering.
abstract: With the ability to form a nano-sized fibrous structure with large pore sizes mimicking the extracellular matrix (ECM), electrospinning was used to fabricate chitosan/poly(vinyl alcohol) nanofibers reinforced by single-walled carbon nanotube (SWNT-CS/PVA) for potential use in neural tissue engineering. Moreover, ultrasonication was performed to fabricate highly dispersed SWNT/CS solution with 7%, 12%, and 17% SWNT content prior to electrospinning process. In the present study, a number of properties of CS/PVA reinforced SWNTs nanocomposites were evaluated. The in vitro biocompatibility of the electrospun fiber mats was also assessed using human brain-derived cells and U373 cell lines. The results have shown that SWNTs as reinforcing phase can augment the morphology, porosity, and structural properties of CS/PVA nanofiber composites and thus benefit the proliferation rate of both cell types. In addition, the cells exhibit their normal morphology while integrating with surrounding fibers. The results confirmed the potential of SWNT-CS/PVA nanocomposites as scaffold for neural tissue engineering.
authors.affiliation: National Cell Bank of Iran, Pasteur Institute of Iran, P O. Box: 13185-1667, Tehran, Iran.
authors.forename: Mohammad Ali
authors.initials: MA
authors.lastname: Shokrgozar
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Mottaghitalab
authors.forename: Vahid
authors.initials: V
authors.lastname: Mottaghitalab
authors.forename: Mehdi
authors.initials: M
authors.lastname: Farokhi
mesh_terms: D045744:Cell Line, Tumor; D048430:Cell Shape; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology; D055664:Electrochemical Techniques; D006801:Humans; D008855:Microscopy, Electron, Scanning; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009417:Nerve Tissue / Q000166:cytology* / Q000187:drug effects; D011142:Polyvinyl Alcohol / Q000737:chemistry* / Q000494:pharmacology; D016062:Porosity; D013010:Sonication; D013059:Spectrum Analysis, Raman; D013718:Tensile Strength; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D037742:Nanotubes, Carbon; D011142:Polyvinyl Alcohol; D048271:Chitosan