title: The milieu of damaged alveolar epithelial type 2 cells stimulates alveolar wound repair by endogenous and exogenous progenitors.
abstract: Alveolar epithelial integrity is dependent upon the alveolar milieu, yet the milieu of the damaged alveolar epithelial cell type 2 (AEC2) has been little studied. Characterization of its components may offer the potential for ex vivo manipulation of stem cells to optimize their therapeutic potential. We examined the cytokine profile of AEC2 damage milieu, hypothesizing that it would promote endogenous epithelial repair while recruiting cells from other locations and instructing their engraftment and differentiation. Bronchoalveolar lavage and lung extract from hyperoxic rats represented AEC2 in vivo damage milieu, and medium from a scratch-damaged AEC2 monolayer represented in vitro damage. CINC-2 and ICAM, the major cytokines detected by proteomic cytokine array in AEC2 damage milieu, were chemoattractive to normoxic AECs and expedited in vitro wound healing, which was blocked by their respective neutralizing antibodies. The AEC2 damage milieu was also chemotactic for exogenous uncommitted human amniotic fluid stem cells (hAFSCs), increasing migration greater than 20-fold. hAFSCs attached within an in vitro AEC2 wound and expedited wound repair by contributing cytokines migration inhibitory factor and plasminogen activator inhibitor 1 to the AEC2 damage milieu, which promoted wound healing. The AEC2 damage milieu also promoted differentiation of a subpopulation of hAFSCs to express SPC, TTF-1, and ABCA3, phenotypic markers of distal alveolar epithelium. Thus, the microenvironment created by AEC2 damage not only promotes autocrine repair but also can attract uncommitted stem cells, which further augment healing through cytokine secretion and differentiation.
authors.affiliation: Developmental Biology and Regenerative Medicine, Saban Research Institute, Childrens Hospital Los Angeles, California, USA.
authors.forename: Susan
authors.initials: S
authors.lastname: Buckley
authors.forename: Wei
authors.initials: W
authors.lastname: Shi
authors.forename: Gianni
authors.initials: G
authors.lastname: Carraro
authors.forename: Sargis
authors.initials: S
authors.lastname: Sedrakyan
authors.forename: Stefano
authors.initials: S
authors.lastname: Da Sacco
authors.forename: Barbara A
authors.initials: BA
authors.lastname: Driscoll
authors.forename: Laura
authors.initials: L
authors.lastname: Perin
authors.forename: Roger E
authors.initials: RE
authors.lastname: De Filippo
authors.forename: David
authors.initials: D
authors.lastname: Warburton
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000096:biosynthesis; D056809:Alveolar Epithelial Cells / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D019898:Autocrine Communication; D002454:Cell Differentiation; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000096:biosynthesis; D005786:Gene Expression Regulation; D006801:Humans; D018496:Hyperoxia / Q000378:metabolism / Q000473:pathology; D008297:Male; D009687:Nuclear Proteins / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration; D013234:Stem Cells / Q000378:metabolism* / Q000473:pathology; D000074482:Thyroid Nuclear Factor 1; D014157:Transcription Factors / Q000096:biosynthesis
chemical_list: C441524:ABCA3 protein, human; D018528:ATP-Binding Cassette Transporters; D016207:Cytokines; D004268:DNA-Binding Proteins; C000614412:NKX2-1 protein, human; C000614416:Nkx2-1 protein, rat; D009687:Nuclear Proteins; C504003:TTF1 protein, human; D000074482:Thyroid Nuclear Factor 1; D014157:Transcription Factors