title: Methanolic extract of leaves of Jasminum grandiflorum Linn modulates oxidative stress and inflammatory mediators.
abstract: The leaves of Jasminum grandiflorum (JG) are in clinical use in Ayurveda for wound management. Since, oxidative stress and inflammation are the primary causes in delayed wound healing, so here its antioxidant and anti-inflammatory activities have been investigated using in vitro as well as in vivo models. The solvent-free methanolic extract of dried leaves of JG were tested for its trapping capacity toward pre-generated ABTS•+ radicals, instantly generated superoxide and hydroxyl radicals, along with metal chelation property, reducing power and total phenolic content. Further, it was tested on LPS-induced nitric oxide and cell viability, on primary culture of rat peritoneal macrophages. Its anti-inflammatory property was also tested on carrageenan-induced paw edema in rats. This extract significantly inhibited iron-induced lipid peroxidation and trapped ABTS•+, superoxide and OH radicals. It significantly inhibited nitric oxide (NO) release, without affecting the cell viability at 800 μg/ml concentration and reduced the formation of paw edema in rats. Thus, it could be suggested that the aforesaid anti-inflammatory properties of JG leaves are associated to its high phenolic content (2.25±0.105 mg/l of gallic acid equivalent), reducing power and its free radical-scavenging property.
authors.affiliation: Department of Medicinal Chemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi, 221005, India.
authors.forename: Adya Prasad
authors.initials: AP
authors.lastname: Chaturvedi
authors.forename: Yamini Bhusan
authors.initials: YB
authors.lastname: Tripathi
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology* / Q000627:therapeutic use; D000975:Antioxidants / Q000494:pharmacology* / Q000627:therapeutic use / Q000633:toxicity; D002351:Carrageenan / Q000633:toxicity; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy; D005260:Female; D016166:Free Radical Scavengers / Q000494:pharmacology / Q000627:therapeutic use / Q000633:toxicity; D017665:Hydroxyl Radical / Q000378:metabolism; D018836:Inflammation Mediators / Q000378:metabolism*; D031660:Jasminum / Q000737:chemistry*; D015227:Lipid Peroxidation; D008264:Macrophages / Q000502:physiology; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010636:Phenols / Q000032:analysis; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use / Q000633:toxicity; D018515:Plant Leaves / Q000737:chemistry; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013481:Superoxides / Q000378:metabolism; D014945:Wound Healing / Q000502:physiology
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D016166:Free Radical Scavengers; D018836:Inflammation Mediators; D010636:Phenols; D010936:Plant Extracts; D013481:Superoxides; D009569:Nitric Oxide; D017665:Hydroxyl Radical; D002351:Carrageenan