title: Rapid determination of gefitinib and its main metabolite, O-desmethyl gefitinib in human plasma using liquid chromatography-tandem mass spectrometry.
abstract: A novel, rapid and specific liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was developed and validated for the simultaneous quantification of gefitinib and its predominant metabolite, O-desmethyl gefitinib in human plasma. Chromatographic separation of analytes was achieved on an Alltima C18 analytical HPLC column (150 mm × 2.1 mm, 5 μm) using an isocratic elution mode with a mobile phase comprised acetonitrile and 0.1% formic acid in water (30:70, v/v). The flow rate was 300 μL/min. The chromatographic run time was 3 min. The column effluents were detected by API 4000 triple quadrupole mass spectrometer using electrospray ionization (ESI) in positive mode. Linearity was demonstrated in the range of 5-1000 ng/mL for gefitinib and 5-500 ng/mL for O-desmethyl gefitinib. The intra- and inter-day precisions for gefitinib and O-desmethyl gefitinib were ≤10.8% and the accuracies ranged from 89.7 to 104.7% for gefitinib and 100.4 to 106.0% for O-desmethyl gefitinib. This method was used as a bioanalytical tool in a phase I clinical trial to investigate the possible effect of hydroxychloroquine on the pharmacokinetics of gefitinib. The results of this study enabled clinicians to ascertain the safety of the combination therapy of hydroxychloroquine and gefitinib in patients with advanced (Stage IIIB-IV) non-small cell lung cancer (NSCLC).
authors.affiliation: Cancer Science Institute of Singapore, National University of Singapore, Singapore. csiwl@nus.edu.sg
authors.forename: Ling-Zhi
authors.initials: LZ
authors.lastname: Wang
authors.forename: Michelle Yi-Xiu
authors.initials: MY
authors.lastname: Lim
authors.forename: Tan-Min
authors.initials: TM
authors.lastname: Chin
authors.forename: Win-Lwin
authors.initials: WL
authors.lastname: Thuya
authors.forename: Pei-Ling
authors.initials: PL
authors.lastname: Nye
authors.forename: Andrea
authors.initials: A
authors.lastname: Wong
authors.forename: Sui-Yung
authors.initials: SY
authors.lastname: Chan
authors.forename: Boon-Cher
authors.initials: BC
authors.lastname: Goh
authors.forename: Paul C
authors.initials: PC
authors.lastname: Ho
mesh_terms: D000970:Antineoplastic Agents / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D002853:Chromatography, Liquid / Q000379:methods*; D004355:Drug Stability; D000077156:Gefitinib; D006801:Humans; D016018:Least-Squares Analysis; D011799:Quinazolines / Q000097:blood* / Q000378:metabolism / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000970:Antineoplastic Agents; D011799:Quinazolines; D000077156:Gefitinib