title: The study of a single BGC823 cell using Fourier transform infrared microspectroscopic imaging.
abstract: In order to investigate gastric cancer at cellular and sub-cellular level, a single human gastric adenocarcinoma BGC823 cell was studied by an infrared microscope equipped with a focal plane array (FPA) detector. The spectra showed difference between the nucleus and the endoplasmic reticulum (ER) of the BGC823 cell. The peak of vasPO2- was shifted to a higher wavenumber at the nucleus compared with that at the ER. The height ratios of 2954 cm(-1)/2922 cm(-1) (CH3/CH2) and 1088 cm(-1)/1539 cm(-1) (DNA/amide II) of the nucleus were significantly higher than those of the ER. Furthermore, chemical images reveal the intensity distributions of lipids, proteins and DNA of the single BGC823 cell, and the intense absorptions of proteins and DNA were observed in the nuclear region of the cell while the intense absorption of lipids was found in the ER region of the cell. The Fourier transform infrared (FTIR) microspectroscopic imaging result indicates the study of the single gastric cancer cell at sub-cellular level can be beneficial for knowing gastric cancer more which will be of great importance for the study and diagnosis of gastric cancer. The result also suggests that FPA is a useful tool in the study of a single cell and may be a powerful tool for study and diagnosis of gastric cancer.
authors.affiliation: National Synchrotron Radiation Laboratory, University of Science and Technology of China, and The First Affiliated Hospital, Hefei, Anhui 230029, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Zeming
authors.initials: Z
authors.lastname: Qi
authors.forename: Shengyi
authors.initials: S
authors.lastname: Wang
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
authors.forename: Helong
authors.initials: H
authors.lastname: Gao
authors.forename: Yangchao
authors.initials: Y
authors.lastname: Tian
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology*; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000648:ultrastructure*; D004247:DNA / Q000648:ultrastructure; D004721:Endoplasmic Reticulum / Q000648:ultrastructure*; D006801:Humans; D008055:Lipids / Q000737:chemistry; D008853:Microscopy / Q000295:instrumentation*; D011506:Proteins / Q000648:ultrastructure; D017550:Spectroscopy, Fourier Transform Infrared; D013274:Stomach Neoplasms / Q000473:pathology*
chemical_list: D008055:Lipids; D011506:Proteins; D004247:DNA