title: Effects of aegeline, a main alkaloid of Aegle Marmelos Correa leaves, on the histamine release from mast cells.
abstract: Aegeline or N-[2-hydroxy-2(4-methoxyphenyl) ethyl]-3-phenyl-2-propenamide is a main alkaloid isolated from Aegle marmelos Correa collected in Yogyakarta Indonesia. In our study, we investigated the effects of aegeline on the histamine release from mast cell. The study was performed by using (1) rat basophilic leukemia (RBL-2H3) cell line, and (2) rat peritoneal mast cells (RPMCs). DNP(24)-BSA, thapsigargin, ionomycin, compound 48/80 and PMA were used as inducers for histamine release from mast cell. In our study, aegeline inhibited the histamine release from RBL-2H3 cells induced by DNP(24)-BSA. Indeed, aegeline showed strong inhibition when RBL-2H3 cells induced by Ca(2+) stimulants such as thapsigargin and ionomycin. Aegeline is suggested to influence the intracellular Ca(2+) pool only since could not inhibit the (45)Ca(2+) influx into RBL-2H3 cells. Aegeline showed weak inhibitory effects on the histamine release from RPMCs, even though still succeed to inhibit when the histamine release induced by thapsigargin. These findings indicate that aegeline altered the signaling pathway related to the intracellular Ca(2+) pool in which thapsigargin acts. Based on the results, the inhibitory effects of aegeline on the histamine release from mast cells depended on the type of mast cell and also involved some mechanisms related to intracellular Ca(2+) signaling events via the same target of the action of thapsigargin or downstream process of intracellular Ca(2+) signaling in mast cells.
authors.affiliation: Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Jogjakarta, Indonesia. agungendronugroho@yahoo.com
authors.forename: Agung Endro
authors.initials: AE
authors.lastname: Nugroho
authors.forename: Sugeng
authors.initials: S
authors.lastname: Riyanto
authors.forename: Mohamad Aspollah
authors.initials: MA
authors.lastname: Sukari
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Maeyama
mesh_terms: D045471:Aegle / Q000737:chemistry*; D000577:Amides / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D004140:Dinitrophenols / Q000037:antagonists & inhibitors / Q000494:pharmacology; D041743:Herb-Drug Interactions; D006636:Histamine Release / Q000187:drug effects*; D015759:Ionomycin / Q000037:antagonists & inhibitors / Q000494:pharmacology; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012710:Serum Albumin, Bovine / Q000037:antagonists & inhibitors / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000037:antagonists & inhibitors / Q000494:pharmacology; D019284:Thapsigargin / Q000037:antagonists & inhibitors / Q000494:pharmacology; D003189:p-Methoxy-N-methylphenethylamine / Q000037:antagonists & inhibitors / Q000494:pharmacology
chemical_list: D000577:Amides; D004140:Dinitrophenols; C034638:dinitrophenyl-bovine serum albumin; D012710:Serum Albumin, Bovine; D003189:p-Methoxy-N-methylphenethylamine; D015759:Ionomycin; C560978:aegeline; D019284:Thapsigargin; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium