title: Polyhemoglobin-superoxide dismutase-catalase-carbonic anhydrase: a novel biotechnology-based blood substitute that transports both oxygen and carbon dioxide and also acts as an antioxidant.
abstract: Polyhemoglobin-superoxide dismutase-catalase-carbonic anhydrase (PolyHb-SOD-CAT-CA) is a therapeutic antioxidant that also transports both oxygen and carbon dioxide. This is formed by crosslinking Hb with SOD, CAT, and CA using glutaraldehyde. Crosslinking stroma-free Hb from red blood cell (RBC) reduces CA activity to 55%. Addition of more CA resulted in a preparation with the same CA activity as RBC. PolyHb in the complex acts as a buffer to prevent large pH changes as carbon dioxide is converted to carbonic acid. We then prepare and optimize a novel PolyHb-SOD-CAT-CA, a therapeutic antioxidant that also transports both oxygen and carbon dioxide.
authors.affiliation: Artificial Cells and Organs Research Centre, Faculty of Medicine, McGill University, Montreal, Quebec, Canada.
authors.forename: Yuzhu
authors.initials: Y
authors.lastname: Bian
authors.forename: Zhixia
authors.initials: Z
authors.lastname: Rong
authors.forename: Thomas Ming Swi
authors.initials: TM
authors.lastname: Chang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001692:Biological Transport; D001709:Biotechnology; D001802:Blood Substitutes / Q000378:metabolism*; D002021:Buffers; D002245:Carbon Dioxide / Q000378:metabolism*; D002256:Carbonic Anhydrases / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism*; D002417:Cattle; D003432:Cross-Linking Reagents / Q000494:pharmacology; D005976:Glutaral / Q000494:pharmacology; D006454:Hemoglobins / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D008970:Molecular Weight; D010100:Oxygen / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D001802:Blood Substitutes; D002021:Buffers; D003432:Cross-Linking Reagents; D006454:Hemoglobins; C016484:hemoglobin, stroma free; C037158:polyhemoglobin; D002245:Carbon Dioxide; D002374:Catalase; D013482:Superoxide Dismutase; D002256:Carbonic Anhydrases; D010100:Oxygen; D005976:Glutaral