title: Detection of lumpy skin disease virus antigen and genomic DNA in formalin-fixed paraffin-embedded tissues from an Egyptian outbreak in 2006.
abstract: An outbreak of lumpy skin disease (LSD) was reported in 2006 in Egypt affecting 16 provinces. Biopsies and post-mortem tissue samples were collected from calves that showed typical clinical signs of LSD and fixed in formalin. These samples were collected from a private dairy farm in the Damietta province of Egypt. Formalin-fixed paraffin-embedded tissue samples were assessed using histology, and skin lesions were classified as either acute or subacute/chronic. Both lumpy skin disease virus (LSDV) DNA detected by polymerase chain reaction and LSDV antigen detected by immunohistochemistry using a capripoxvirus-specific monoclonal antibody were observed in the acute skin lesions and in some subacute/chronic skin lesions.
authors.affiliation: Department of Pathology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt. walaaaa2006@yahoo.com
authors.forename: W
authors.initials: W
authors.lastname: Awadin
authors.forename: H
authors.initials: H
authors.lastname: Hussein
authors.forename: Y
authors.initials: Y
authors.lastname: Elseady
authors.forename: S
authors.initials: S
authors.lastname: Babiuk
authors.forename: H
authors.initials: H
authors.lastname: Furuoka
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000302:isolation & purification*; D002417:Cattle; D004279:DNA, Viral / Q000302:isolation & purification*; D003612:Dairying; D004534:Egypt / Q000453:epidemiology; D005260:Female; D005557:Formaldehyde; D008166:Lumpy Skin Disease / Q000175:diagnosis* / Q000453:epidemiology; D008167:Lumpy skin disease virus / Q000235:genetics* / Q000276:immunology*; D008297:Male; D016612:Paraffin Embedding / Q000662:veterinary*; D013997:Time Factors; D014021:Tissue Preservation / Q000379:methods / Q000662:veterinary
chemical_list: D000956:Antigens, Viral; D004279:DNA, Viral; D005557:Formaldehyde