title: Two novel monoclonal antibodies produced against human CD83 molecule.
abstract: CD83, a maturation marker for human and mouse dendritic cells (DCs), plays a critical role in CD4(+) T cell development as well as peripheral immune regulation. Here, two novel mouse anti-human CD83 monoclonal antibodies (MAbs) were prepared and their immunological characteristics were determined. Among the two MAbs, 8B4 binds to a linear epitope whereas 1E11 recognizes a conformational epitope. Cross-linking of 8B4 but not 1E11 with CD83-Ig augments the fusion protein mediated inhibition of peripheral blood mononuclear cells (PBMCs). Thus the two MAbs may be good candidates for immunoassaying and functional exploration of CD83 molecule.
authors.affiliation: Institute of Medical Biotechnology, Soochow University, Suzhou, China.
authors.forename: Liwen
authors.initials: L
authors.lastname: Chen
authors.forename: Chao
authors.initials: C
authors.lastname: Gao
authors.forename: Yibei
authors.initials: Y
authors.lastname: Zhu
authors.forename: Weixue
authors.initials: W
authors.lastname: Zhong
authors.forename: Yumin
authors.initials: Y
authors.lastname: Hu
authors.forename: Xueguang
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D058846:Antibodies, Monoclonal, Murine-Derived / Q000096:biosynthesis* / Q000276:immunology* / Q000378:metabolism; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology*; D002460:Cell Line; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D018984:Epitopes, T-Lymphocyte / Q000378:metabolism; D006801:Humans; D007136:Immunoglobulins / Q000235:genetics / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008562:Membrane Glycoproteins / Q000235:genetics / Q000276:immunology*; D051379:Mice; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000097802:CD83 Antigen
chemical_list: D058846:Antibodies, Monoclonal, Murine-Derived; D015703:Antigens, CD; D017931:DNA Primers; D018076:DNA, Complementary; D018984:Epitopes, T-Lymphocyte; D007136:Immunoglobulins; D008562:Membrane Glycoproteins; D000097802:CD83 Antigen