title: Study of pharmacokinetics and tissue distribution of liposomal brucine for dermal administration.
abstract: OBJECTIVE
  To evaluate the pharmacokinetics and tissue distribution of liposomal brucine (LB) for dermal application.
  
  
  METHODS
  Pharmacokinetics and tissue distribution were studied by in vivo animal testing. High performance liquid chromatography (HPLC) was used to detect the concentration of brucine in rats' skin, plasma and various tissues.
  
  
  RESULTS
  After dermal administration, LB was absorbed rapidly in the skin and could be detected after 0.5 hours. After 36 hours, levels were too low to be detected. In plasma, levels were also too low to be detected after 36 hours. The concentration of LB reached 50% of the maximum in all tissues except the brain, peaking after 1.5 hours but still detectable after 12 hours.
  
  
  CONCLUSIONS
  The concentration of LB was high in skin at the application site. LB was quickly absorbed into tissues through the blood circulation and widely distributed throughout the whole body. There was no obvious toxicity and LB did not readily accumulate in tissues and organs. It showed local potency but low overall systemic toxicity.
authors.affiliation: Pharmacy Department of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, People's Republic of China. ybc2002@sina.com
authors.forename: Bai-Can
authors.initials: BC
authors.lastname: Yang
authors.forename: Zhi-Feng
authors.initials: ZF
authors.lastname: Chu
authors.forename: Sha
authors.initials: S
authors.lastname: Zhu
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Wang
authors.forename: Yu-Hong
authors.initials: YH
authors.lastname: Feng
authors.forename: Feng-Hua
authors.initials: FH
authors.lastname: Li
authors.forename: Chang-Sheng
authors.initials: CS
authors.lastname: Liu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yuan
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006207:Half-Life; D008081:Liposomes; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000737:chemistry / Q000378:metabolism; D013331:Strychnine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics; D014018:Tissue Distribution
chemical_list: D008081:Liposomes; C083806:brucine; D013331:Strychnine