title: Effect of Growth Regulators on In Vitro Morphogenic Response of Boscia senegalensis (Pers.) Lam. Poir. Using Mature Zygotic Embryos Explants.
abstract: The percent study describes the in vitro responses of mature zygotic embryos of Boscia senegalensis to different concentrations (0.0-5.0 mg/L) of 6-benzyladnine (BA), Thidiazuron (TDZ), α-Naphthalene acetic acid (NAA), and 2, 4-Dichlorophenoxyacetic acid (2, 4-D) supplemented on Murashige and Skoog medium (MS). The plant growth regulators (PGRs) were considerably affected the morphogenetic responses. BA produced adventitious shoots through two ways: direct organogenesis and auxiliary shoot formation. Both 2, 4-D and TDZ tend to produce callus, whereas NAA improve the development of embryos to seedlings. Maximum number of shoots/explant (14.8 ± 0.6) was obtained on MS medium supplemented with 3.0 mg/L BA. 67.0% of excised shoots were rooted either on 1/2 MS medium augmented with or without 0.25 mg/L IBA. The highest number of roots (1.2 ± 0.4) and root length (0.5 ± 0.2 cm) was produced on 0.25 mg/L IBA-containing medium. Regenerated plants were successfully acclimatized and transferred to the green house with 70% survival rate. All the plants appeared morphologically uniform with normal growth pattern. A rapid (30 days), efficient and without subculturing protocol for in vitro regeneration of B. senegalensis was developed.
authors.affiliation: Commission for Biotechnology and Genetic Engineering, National Centre for Research, P.O. Box: 2404, Khartoum, Sudan.
authors.forename: Hussien H
authors.initials: HH
authors.lastname: Daffalla
authors.forename: Eltayb
authors.initials: E
authors.lastname: Abdellatef
authors.forename: Elsadig A
authors.initials: EA
authors.lastname: Elhadi
authors.forename: Mutasim M
authors.initials: MM
authors.lastname: Khalafalla