title: Human papillomavirus 13 in a Mexican Mayan community with multifocal epithelial hyperplasia: could saliva be involved in household transmission?
abstract: Multifocal epithelial hyperplasia (MEH) is a disease of the oral mucosa. Human papillomaviruses 13 and 32 have been detected in these lesions. We describe the epidemiology and clinical characteristics of patients with MEH in a rural community in the Mayan area of Mexico with 53 cases and 54 controls. Clinical and epidemiological data were collected through a direct interview. Oral cell samples were collected with a cytobrush. Subjects collected their own saliva sample in a sterile bottle. All samples were tested for HPV 13 and 32 by polymerase chain reaction using specific primers. Of the 53 patients and 54 healthy subjects, 56% were < 12 years old, 25% were males and 75% females. Evolution of the lesions was between two months and 17 years. The lesions affected lips, jugal mucosa, and tongue, 96% had multiple lesions. From 53 patients, fifty samples of oral cells and 31 samples of saliva were analyzed. HPV 13 was detected in 100% oral cell and 100% saliva samples studied. 16 healthy subjects were HVP 13 positive. A highly significant association of HPV 13 infection and MEH was found, as determined by chi square test (p = 0.00) Household transmission of HPV 13 may happen through saliva and the shared use of contaminated objects.
authors.affiliation: Dentistry School, University of Yucatan, Merida Yucatan, México.
authors.forename: Maria Eugenia
authors.initials: ME
authors.lastname: Lopez-Villanueva
authors.forename: Laura
authors.initials: L
authors.lastname: Conde-Ferráez
authors.forename: Guadalupe
authors.initials: G
authors.lastname: Ayora-Talavera
authors.forename: Jose D
authors.initials: JD
authors.lastname: Cerón-Espinosa
authors.forename: Maria del Refugio
authors.initials: Mdel R
authors.lastname: González-Losa
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral / Q000032:analysis*; D003937:Diagnosis, Differential; D005260:Female; D017573:Focal Epithelial Hyperplasia / Q000208:ethnology / Q000473:pathology* / Q000821:virology; D006801:Humans; D006965:Hyperplasia; D015994:Incidence; D008297:Male; D008800:Mexico / Q000453:epidemiology; D008875:Middle Aged; D009061:Mouth Mucosa / Q000473:pathology* / Q000821:virology; D009062:Mouth Neoplasms / Q000208:ethnology / Q000473:pathology* / Q000821:virology; D027383:Papillomaviridae / Q000235:genetics*; D030361:Papillomavirus Infections / Q000453:epidemiology / Q000821:virology; D016133:Polymerase Chain Reaction; D012189:Retrospective Studies; D012463:Saliva / Q000821:virology; D055815:Young Adult
chemical_list: D004279:DNA, Viral