title: Corrosion of Mg alloy AZ91D in the presence of living cells.
abstract: Mg and Mg alloys are of interest for biodegradable implants as they readily corrode in biological fluids, and dissolved Mg ions are nontoxic. Even though it is well known that Mg dissolution leads to pH increase in the surroundings, the effect of the corrosion-induced alkalization on the biological environment has not been studied in detail. We therefore explored the interactions between corrosion-induced pH increase and cell growth on Mg alloy AZ91D surface. Cell adhesion and spreading on the alloy surface is unimpeded initially. However, with time a large fraction of cells de-adhere. We attribute this to the observed increase of the pH in the cell culture medium in the process of alloy dissolution. Cytotoxicity tests with HeLa cells grown on glass surfaces confirm that cell death increases with increasing alkalinity of the cell culture medium. We also show that a the cells that adhere on the Mg alloy surface act as a corrosion-blocking surface layer. In consequence, a slower pH increase in the medium takes place when the alloy surface is covered with cells. Electrochemical impedance spectroscopy measurements (EIS) verify that a cell layer slows down the corrosion process.
authors.affiliation: Department of Materials Science and Engineering, WW4-LKO, University of Erlangen-Nuremberg, Martensstraβe 7, 91058 Erlangen, Germany.
authors.forename: F
authors.initials: F
authors.lastname: Seuss
authors.forename: S
authors.initials: S
authors.lastname: Seuss
authors.forename: M C
authors.initials: MC
authors.lastname: Turhan
authors.forename: B
authors.initials: B
authors.lastname: Fabry
authors.forename: S
authors.initials: S
authors.lastname: Virtanen
mesh_terms: D020341:Absorbable Implants; D000497:Alloys / Q000737:chemistry*; D001672:Biocompatible Materials / Q000032:analysis; D002448:Cell Adhesion; D049109:Cell Proliferation; D003343:Corrosion; D003470:Culture Media / Q000494:pharmacology; D058266:Dielectric Spectroscopy / Q000379:methods; D004563:Electrochemistry / Q000379:methods; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000737:chemistry*; D008422:Materials Testing; D013499:Surface Properties; D013997:Time Factors
chemical_list: D000497:Alloys; D001672:Biocompatible Materials; D003470:Culture Media; D008274:Magnesium