title: [Effect of 5-fluorouracil aerosol on the growth of human gastric cancer cell line MKN-45 in vitro].
abstract: OBJECTIVE
  To evaluate the effect of simulated intraperitoneal 5-fluorouracil (5-Fu) aerosol chemotherapy (AIPC) on the proliferation, apoptosis, and cell cycle of human gastric cancer cell line MKN-45 in vitro.
  
  
  METHODS
  The gastric cancer cells MKN-45 were treated with 5-Fu aerosol for 30 min under the pressure of 8 mmHg, and those treated with normal saline (NS) aerosol served as the control. The cell proliferation after the treatment was detected by MTT assay, and flow cytometry and FITC Annexin V/PI kit were used to detect the cell apoptosis and changes in the cell cycle.
  
  
  RESULTS
  MTT assay showed a significantly greater inhibition rate of the cell proliferation in 5-Fu aerosol group than in NS group [(31.13∓3.51)% vs (4.65∓1.99)%, P<0.001]. FCM analysis also showed a significantly higher cell apoptotic rate in 5-Fu aerosol group than in NS group [(12.00∓0.92)% vs (2.65∓0.52)%, P<0.001]. Compared with saline treatment, treatment with 5-Fu aerosol resulted in a greater proportion of G1 phase cells [(51.83∓1.95)% vs (36.41∓2.33)%, P<0.001] with a lowered proportion of S phase cells [(16.72∓2.36)% vs (45.20∓3.27)%, P<0.001].
  
  
  CONCLUSIONS
  Simulated 5-Fu AIPC can inhibit the proliferation, induce cell apoptosis and cause cell cycle arrest at G1 phase in gastric cancer cells.
authors.affiliation: Department of General Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. lintian108@163.com
authors.forename: Tian
authors.initials: T
authors.lastname: Lin
authors.forename: Jiang
authors.initials: J
authors.lastname: Yu
authors.forename: Zhi-gang
authors.initials: ZG
authors.lastname: Wei
authors.forename: Yan-feng
authors.initials: YF
authors.lastname: Hu
authors.forename: Ya-nan
authors.initials: YN
authors.lastname: Wang
authors.forename: Guo-xin
authors.initials: GX
authors.lastname: Li
mesh_terms: D000336:Aerosols / Q000008:administration & dosage / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D005472:Fluorouracil / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D013274:Stomach Neoplasms / Q000473:pathology*
chemical_list: D000336:Aerosols; D005472:Fluorouracil