title: Toll-like receptor-2-activating bifidobacteria strains differentially regulate inflammatory cytokines in the porcine intestinal epithelial cell culture system: finding new anti-inflammatory immunobiotics.
abstract: A total of 23 strains of bifidobacteria taxonomically belonging to five species were tested for their potent immunomodulatory effect using a combination of two methods: the NF-κB-reporter assay using a toll-like receptor 2-expressing transfectant (HEK(pTLR2) system) and the mitogenic assay using porcine Peyer's patches immunocompetent cells. Among the four preselected strains from different immunomodulatory groups, Bifidobacterium breve MCC-117 was able to efficiently modulate the inflammatory response triggered by enterotoxigenic Escherichia coli (ETEC) in a porcine intestinal epithelial (PIE) cell line. Moreover, using PIE cells and swine Peyer's patches immunocompetent cell co-culture system, we demonstrated that the immunoregulatory effect of B. breve MCC-117 was related to the capacity of the strain to influence PIE and immune cell interactions, leading to the stimulation of regulatory T cells. The results suggested that bifidobacteria that express high activity in both the HEK(pTLR2) and the mitogenic assays may behave like potential anti-inflammatory strains. The combination of the HEK(pTLR2) system, the evaluation of mitogenic activity and PIE cells will be of value for the development of new immunologically functional foods and feeds that could prevent inflammatory intestinal disorders. Although our findings should be proven in appropriate experiments in vivo, the results of the present work provide a scientific rationale for the use of B. breve MCC-117 to prevent ETEC-induced intestinal inflammation.
authors.affiliation: Food Immunology Group, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
authors.forename: Hitomi
authors.initials: H
authors.lastname: Fujie
authors.forename: Julio
authors.initials: J
authors.lastname: Villena
authors.forename: Masanori
authors.initials: M
authors.lastname: Tohno
authors.forename: Kyoko
authors.initials: K
authors.lastname: Morie
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Shimazu
authors.forename: Hisashi
authors.initials: H
authors.lastname: Aso
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Suda
authors.forename: Takeshi
authors.initials: T
authors.lastname: Shimosato
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Iwabuchi
authors.forename: Jin-Zhong
authors.initials: JZ
authors.lastname: Xiao
authors.forename: Tomoko
authors.initials: T
authors.lastname: Yaeshima
authors.forename: Keiji
authors.initials: K
authors.lastname: Iwatsuki
authors.forename: Tadao
authors.initials: T
authors.lastname: Saito
authors.forename: Muneo
authors.initials: M
authors.lastname: Numasaki
authors.forename: Haruki
authors.initials: H
authors.lastname: Kitazawa
mesh_terms: D000818:Animals; D001644:Bifidobacterium / Q000276:immunology*; D002478:Cells, Cultured; D018920:Coculture Techniques; D016207:Cytokines / Q000096:biosynthesis*; D004847:Epithelial Cells / Q000276:immunology* / Q000382:microbiology*; D004926:Escherichia coli / Q000472:pathogenicity; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D017930:Genes, Reporter; D007249:Inflammation / Q000276:immunology / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control; D007413:Intestinal Mucosa / Q000276:immunology / Q000382:microbiology; D060888:Real-Time Polymerase Chain Reaction; D013552:Swine; D051195:Toll-Like Receptor 2 / Q000276:immunology*
chemical_list: D016207:Cytokines; D051195:Toll-Like Receptor 2