title: Cell proliferation and neuroblast differentiation in the rat dentate gyrus after intrathecal treatment with adipose-derived mesenchymal stem cells.
abstract: Mesenchymal stem cells (MSC) have emerged as a new therapeutic tool for a number of clinical applications, because they have multipotency and paracrine effects via various factors. In the present study, we investigated the effects of adipose-derived MSC (Ad-MSC) transplantation via intrathecal injection through the cisterna magna on cell proliferation and differentiation of endogenous stem cells in the hippocampal dentate gyrus (DG) using Ki-67 (a marker for proliferating cells), and doublecortin (DCX, a marker for neuroblasts). The transplanted Ad-MSC were detected in the meninges, not in the hippocampal parenchyma. However, the number of Ki-67-immunoreactive cells was significantly increased by 83% in the DG 2 days after single Ad-MSC injection, and by 67% at 23 days after repeated Ad-MSC treatment compared with that in the vehicle-treated group after Ad-MSC transplantation. On the other hand, the number of DCX-immunoreactive cells in the DG was not changed at 2 days after single Ad-MSC injection; however, it was significantly increased by 62% 9 days after single Ad-MSC injection. At 23 days after repeated Ad-MSC application, the number of DCX-immunoreactive cells was much more increased (223% of the vehicle-treated group). At this time point, DCX protein levels were also significantly increased compared with those in the vehicle-treated group. These results suggest that the intrathecal injection of Ad-MSC could enhance endogenous cell proliferation, and the repeated Ad-MSC injection could be more efficient for an enhancement of endogenous cell proliferation and differentiation in the brain.
authors.affiliation: Department of Anatomy, College of Veterinary Medicine, Kangwon National University, Chuncheon 200-701, South Korea.
authors.forename: Jung Hoon
authors.initials: JH
authors.lastname: Choi
authors.forename: Jin Young
authors.initials: JY
authors.lastname: Chung
authors.forename: Dae Young
authors.initials: DY
authors.lastname: Yoo
authors.forename: In Koo
authors.initials: IK
authors.lastname: Hwang
authors.forename: Ki-Yeon
authors.initials: KY
authors.lastname: Yoo
authors.forename: Choong Hyun
authors.initials: CH
authors.lastname: Lee
authors.forename: Bing Chun
authors.initials: BC
authors.lastname: Yan
authors.forename: Jin Ok
authors.initials: JO
authors.lastname: Ahn
authors.forename: Hwa Young
authors.initials: HY
authors.lastname: Youn
authors.forename: Moo-Ho
authors.initials: MH
authors.lastname: Won
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology*; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation; D018891:Dentate Gyrus / Q000166:cytology / Q000502:physiology*; D000089662:Doublecortin Protein; D007278:Injections, Spinal; D019394:Ki-67 Antigen / Q000378:metabolism; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D015415:Biomarkers; C000714848:Dcx protein, rat; D000089662:Doublecortin Protein; D019394:Ki-67 Antigen