title: The effects of hyaluronic acid, calcium hydroxide, and dentin adhesive on rat odontoblasts and fibroblasts.
abstract: The aim of this study was to investigate the effects and efficiency of pulp capping preparations based on hyaluronic acid, calcium hydroxide, and dentin adhesive on the pulp tissue of Sprague-Dawley rats. The rats were killed and extracted teeth sectioned transversely through the pulp. The slices were placed in a RPMI 1640 cell culture medium supplemented with 10 % foetal calf serum. During 14 days of cultivation cultures were treated with preparations that contained hyaluronic acid (Gengigel Prof®), and calcium hydroxide (ApexCal®), or with dentin adhesive (Excite®). Cellularity and viability of fibroblasts and odontoblasts was analysed using a haemocytometer. Hyaluronic acid proved most efficient and the least toxic for direct pulp capping. Even though calcium hydroxide and dentin adhesive demonstrated a higher degree of cytotoxicity, their effects were still acceptable in terms of biocompatibility.
authors.affiliation: University of Zagreb School of Dental Medicine, Croatia.
authors.forename: Ana
authors.initials: A
authors.lastname: Bogović
authors.forename: Jana
authors.initials: J
authors.lastname: Nižetić
authors.forename: Nada
authors.initials: N
authors.lastname: Galić
authors.forename: Davor
authors.initials: D
authors.lastname: Zelježić
authors.forename: Vedran
authors.initials: V
authors.lastname: Micek
authors.forename: Marin
authors.initials: M
authors.lastname: Mladinić
mesh_terms: D000818:Animals; D002126:Calcium Hydroxide / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D003782:Dental Pulp / Q000187:drug effects*; D003785:Dental Pulp Capping; D017220:Dentin-Bonding Agents / Q000494:pharmacology*; D005260:Female; D005347:Fibroblasts / Q000187:drug effects*; D006820:Hyaluronic Acid / Q000494:pharmacology*; D008297:Male; D008689:Methacrylates / Q000494:pharmacology; D009804:Odontoblasts / Q000187:drug effects*; D057872:Pulp Capping and Pulpectomy Agents / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012387:Root Canal Filling Materials / Q000494:pharmacology
chemical_list: C546890:ApexCal; D017220:Dentin-Bonding Agents; C420130:Excite cement; D008689:Methacrylates; D057872:Pulp Capping and Pulpectomy Agents; D012387:Root Canal Filling Materials; D006820:Hyaluronic Acid; D002126:Calcium Hydroxide