title: [Simultaneous determination of five glycosides in Aidi injection by RP-HPLC-ELSD].
abstract: OBJECTIVE
  To develop a RP-HPLC-ELSD method for the simultaneous determination of five glycosides in Aidi injection.
  
  
  METHODS
  The separation was carried out at 35 degrees C on a Kromasil C18 column (4.6 mm x 250 mm, 5 microm) eluted with acetonitrile and water as the mobile phases in gradient mode. The flow rate was 1.0 mL x min(-1). The SHIMADZU ELSD-LT II detector was used. The detector temperature was at 40 degrees C and the pressure of carrier gas N2 was 0.35 MPa.
  
  
  RESULTS
  The linear response (the natural logarithm of peak areas with corresponding mass) ranges were 21.2-212 mg x L(-1) for syringin, 30.8-308 mg x L(-1) for ginsenoside Rg1, 24.8-248 mg x L(-1) for ginsenoside Re, 25.6-256 mg x L(-1) for ginsenoside Rb1, 31.2-312 mg x L(-1), for astragaloside IV, respectively. The average recoveries (n = 9) of five glycosides were greater than 97.6%, and RSD were less than 1.1%.
  
  
  CONCLUSIONS
  The results demonstrated that this method had adequate accuracy and selectivity to measure the concentrations of five glycosides in Aidi injection.
authors.affiliation: College of Pharmaceutical Science, Soochow University, Suzhou 215123, China.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Chen
authors.forename: Miaomiao
authors.initials: M
authors.lastname: Zhang
authors.forename: Xiaoran
authors.initials: X
authors.lastname: Li
authors.forename: Qiongming
authors.initials: Q
authors.lastname: Xu
authors.forename: Shilin
authors.initials: S
authors.lastname: Yang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D036145:Ginsenosides / Q000032:analysis; D005960:Glucosides / Q000032:analysis; D006027:Glycosides / Q000032:analysis*; D007267:Injections; D010666:Phenylpropionates / Q000032:analysis; D012503:Saponins / Q000032:analysis; D014315:Triterpenes / Q000032:analysis
chemical_list: D004365:Drugs, Chinese Herbal; D036145:Ginsenosides; D005960:Glucosides; D006027:Glycosides; D010666:Phenylpropionates; D012503:Saponins; D014315:Triterpenes; C517300:aidi herbal drug; C052064:astragaloside A; C049864:ginsenoside Re; C442759:ginsenoside Rb1; C028305:syringin; C035054:ginsenoside Rg1