title: Versatile method for the detection of covalently bound substrates on solid supports by DART mass spectrometry.
abstract: Analysis of substrates directly on solid phase resins without the need for separate cleavage conditions remains an outstanding challenge in the field of solid phase synthesis. We now present the first example of simultaneous cleavage and mass spectrometric analysis of peptides from solid supports using direct analysis in real time (DART) mass spectrometry. We have shown that this method is compatible with a diverse array of solid phase resins and is suitable for analysis of both peptides and organic substrates.
authors.affiliation: Department of Chemistry and Biochemistry, University of California Santa Cruz, Santa Cruz, California 95064, USA.
authors.forename: Laura M
authors.initials: LM
authors.lastname: Sanchez
authors.forename: Matthew E
authors.initials: ME
authors.lastname: Curtis
authors.forename: Bianca E
authors.initials: BE
authors.lastname: Bracamonte
authors.forename: Kenji L
authors.initials: KL
authors.lastname: Kurita
authors.forename: Gabriel
authors.initials: G
authors.lastname: Navarro
authors.forename: O David
authors.initials: OD
authors.lastname: Sparkman
authors.forename: Roger G
authors.initials: RG
authors.lastname: Linington
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D015394:Molecular Structure; D010455:Peptides / Q000032:analysis* / Q000737:chemistry
chemical_list: D000596:Amino Acids; D010455:Peptides