title: Peptide nucleic acid probes with charged photocleavable mass markers: Towards PNA-based MALDI-TOF MS genetic analysis.
abstract: Halogen-labelled peptide organic acid (HPOA) monomers have been synthesised and incorporated into sequence-specific peptide nucleic acid (PNA) probes. Three different types of probe have been prepared; the unmodified PNA probe, the PNA probe with a mass marker, and the PNA probe with photocleavable mass marker. All three types of probe have been used in model studies to develop a mass spectrometry-based hybridisation assay for detection of point mutations in DNA.
authors.affiliation: School of Chemistry; University of Southampton; Southampton, UK.
authors.forename: Rachel J
authors.initials: RJ
authors.lastname: Ball
authors.forename: Philip S
authors.initials: PS
authors.lastname: Green
authors.forename: Nittaya
authors.initials: N
authors.lastname: Gale
authors.forename: G John
authors.initials: GJ
authors.lastname: Langley
authors.forename: Tom
authors.initials: T
authors.lastname: Brown