title: cis-Acting effects on RNA processing and Drosha cleavage prevent Epstein-Barr virus latency III BHRF1 expression.
abstract: In Epstein-Barr virus (EBV) latency III (LTIII) infection, BHRF1 encodes three microRNAs (miRNAs). Herein we report that Drosha cleavage of LTIII BHRF1 RNA and cis-acting splicing effects inhibit splicing and inhibit BHRF1 RNA and protein expression. Evidence shown here supports the view that Drosha cleavage to generate mature miRNAs and cis-acting sequences that prevent mRNA maturation are independent processes that prevent LTIII BHRF1 expression in lymphoblastoid cell lines.
authors.affiliation: Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA. xingli107@gmail.com
authors.forename: Li
authors.initials: L
authors.lastname: Xing
authors.forename: Elliott
authors.initials: E
authors.lastname: Kieff
mesh_terms: D002460:Cell Line; D004854:Herpesvirus 4, Human / Q000472:pathogenicity*; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis*; D012323:RNA Processing, Post-Transcriptional; D043244:Ribonuclease III / Q000378:metabolism*; D014764:Viral Proteins / Q000096:biosynthesis*; D017735:Virus Latency
chemical_list: C083278:BHRF1 protein, Human herpesvirus 4; D035683:MicroRNAs; D014764:Viral Proteins; C517918:DROSHA protein, human; D043244:Ribonuclease III