title: Flow cytometry as a diagnostic tool in the early diagnosis of aggressive lymphomas mimicking life-threatening infection.
abstract: Aggressive lymphomas can present with symptoms mimicking life-threatening infection. Flow cytometry (FC) is usually recommended for the classification and staging of lymphomas in patients with organomegaly and atypical cells in effusions and blood, after the exclusion of other possible diagnoses. FC may also have a place in the initial diagnostic investigation of aggressive lymphoma. Three cases are presented here of highly aggressive lymphomas in young adults, which presented with the clinical picture of fever of unknown origin (FUO) in patients severely ill. All followed a life-threatening clinical course, and two developed the hemophagocytic syndrome (HPS), but microbiological, immunological, and morphological evaluation and immunohistochemistry (IHC) failed to substantiate an early diagnosis. FC was the technique that provided conclusive diagnostic evidence of lymphoma, subsequently verified by IHC. Our experience with these three cases highlights the potential role of FC as an adjunct methodology in the initial assessment of possible highly aggressive lymphoma presenting with the signs and symptoms of life-threatening infection, although the definitive diagnosis should be established by biopsy. In such cases, FC can contribute to the diagnosis of lymphoma, independently of the presence of HPS.
authors.affiliation: Flow Cytometry Laboratory, Department of Immunology, Athens Regional General Hospital "G. Gennimatas", 11527 Athens, Greece.
authors.forename: Nikolaos J
authors.initials: NJ
authors.lastname: Tsagarakis
authors.forename: Nektaria A
authors.initials: NA
authors.lastname: Kentrou
authors.forename: Georgios
authors.initials: G
authors.lastname: Kakiopoulos
authors.forename: Georgios
authors.initials: G
authors.lastname: Androutsos
authors.forename: Athanasios
authors.initials: A
authors.lastname: Galanopoulos
authors.forename: Christos
authors.initials: C
authors.lastname: Michaelidis
authors.forename: Dimitra
authors.initials: D
authors.lastname: Rontogianni
authors.forename: Apostolos
authors.initials: A
authors.lastname: Tolis
authors.forename: Stavroula
authors.initials: S
authors.lastname: Chini
authors.forename: Georgios
authors.initials: G
authors.lastname: Gortzolidis
authors.forename: Konstantinos A
authors.initials: KA
authors.lastname: Papadimitriou
authors.forename: Dimitra
authors.initials: D
authors.lastname: Skoumi
authors.forename: Konstantina
authors.initials: K
authors.lastname: Tzanetou
authors.forename: Georgios
authors.initials: G
authors.lastname: Paterakis