title: Tooth tissue engineering: optimal dental stem cell harvest based on tooth development.
abstract: Our long-term objective is to devise reliable methods to generate biological replacement teeth exhibiting the physical properties and functions of naturally formed human teeth. Previously, we demonstrated the successful use of tissue engineering approaches to generate small, bioengineered tooth crowns from harvested pig and rat postnatal dental stem cells (DSCs). To facilitate characterizations of human DSCs, we have developed a novel radiographic staging system to accurately correlate human third molar tooth developmental stage with anticipated harvested DSC yield. Our results demonstrated that DSC yields were higher in less developed teeth (Stages 1 and 2), and lower in more developed teeth (Stages 3, 4, and 5). The greatest cell yields and colony-forming units (CFUs) capability was obtained from Stages 1 and 2 tooth dental pulp. We conclude that radiographic developmental staging can be used to accurately assess the utility of harvested human teeth for future dental tissue engineering applications.
authors.affiliation: Interdisciplinary Center of Gene Therapy, UNIFESP-CINTERGEN, Department of Plastic Surgery, Federal University of São Paulo, Brazil.
authors.forename: Monica Talarico
authors.initials: MT
authors.lastname: Duailibi
authors.forename: Silvio Eduardo
authors.initials: SE
authors.lastname: Duailibi
authors.forename: Eduardo Felippe
authors.initials: EF
authors.lastname: Duailibi Neto
authors.forename: Renata Matalon
authors.initials: RM
authors.lastname: Negreiros
authors.forename: Waldyr Antonio
authors.initials: WA
authors.lastname: Jorge
authors.forename: Lydia Masako
authors.initials: LM
authors.lastname: Ferreira
authors.forename: Joseph Phillip
authors.initials: JP
authors.lastname: Vacanti
authors.forename: Pamela Crotty
authors.initials: PC
authors.lastname: Yelick
mesh_terms: D000293:Adolescent; D000328:Adult; D002478:Cells, Cultured; D002648:Child; D005260:Female; D006801:Humans; D008297:Male; D008964:Molar, Third / Q000166:cytology* / Q000000981:diagnostic imaging / Q000254:growth & development*; D009805:Odontogenesis; D011859:Radiography; D013234:Stem Cells / Q000166:cytology*; D023822:Tissue Engineering / Q000379:methods*; D055815:Young Adult