title: Caveolin-1 inhibits oligodendroglial differentiation of neural stem/progenitor cells through modulating β-catenin expression.
abstract: In the present study, we aim to elucidate the role of caveolin-1 (Cav-1) in modulating oligodendroglial differentiation of neural progenitor cells (NPCs) in vivo and in vitro. For in vivo experiments, we investigated oligodendroglial differentiation by detecting the expressions of 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) and β-catenin in the brains of wild type mice and Cav-1 knockout mice. Cav-1 knockout mice revealed more oligodendroglial differentiation, but lower levels of β-catenin expression than wild type mice. For in vitro experiments, we observed the potential roles of Cav-1 in modulating β-catenin expression and oligodendroglial differentiation in isolated cultured NPCs by manipulating Cav-1 expression with Cav-1 scaffolding domain peptide and Cav-1 RNA silencing approach. In the differentiating NPCs, Cav-1 scaffolding domain peptide markedly inhibited oligodendroglial formation, but up-regulated the expression of β-catenin. In contrast, the knockdown of Cav-1 promoted oligodendroglial differentiation of NPCs, but down-regulated the expression of β-catenin. Taken together, these results directly prove that caveolin-1 can inhibit oligodendroglial differentiation of NPCs through modulating β-catenin expression.
authors.affiliation: School of Chinese Medicine, The University of Hong Kong, Hong Kong Special Administrative Region.
authors.forename: Yue
authors.initials: Y
authors.lastname: Li
authors.forename: Wui-Man
authors.initials: WM
authors.lastname: Lau
authors.forename: Kwok-Fai
authors.initials: KF
authors.lastname: So
authors.forename: Yao
authors.initials: Y
authors.lastname: Tong
authors.forename: Jiangang
authors.initials: J
authors.lastname: Shen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D051242:Caveolin 1 / Q000737:chemistry / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D008969:Molecular Sequence Data; D009836:Oligodendroglia / Q000166:cytology* / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology*; D051176:beta Catenin / Q000378:metabolism*
chemical_list: D051242:Caveolin 1; D051176:beta Catenin