title: Reconstituted high-density lipoprotein shortens cardiac repolarization.
abstract: OBJECTIVE
  We hypothesize that increasing high-density lipoprotein cholesterol (HDL-C) shortens cardiac repolarization.
  
  
  BACKGROUND
  HDL-C is inversely associated with sudden death. The relation between HDL-C and repolarization of the heart is unexplored.
  
  
  METHODS
  HDL-C was elevated with reconstituted high-density lipoprotein (rHDL). Cardiac repolarization was studied by recording cardiac transmembrane potentials with the patch clamp technique from isolated rabbit cardiomyocytes that were superfused with rHDL. Infusions with rHDL (40 mg/kg body weight) were performed in dyslipidemic patients and healthy volunteers. Electrocardiograms were recorded to assess cardiac repolarization before and 24 h after infusion with rHDL.
  
  
  RESULTS
  rHDL as well as purified human apolipoprotein AI shortened repolarization of isolated rabbit cardiomyocytes by ∼25% (p < 0.05). rHDL infusion shortened the heart rate-corrected QT interval on surface electrocardiograms in all participants (p < 0.001).
  
  
  CONCLUSIONS
  rHDL shortens cardiac repolarization. These data provide evidence for a novel mechanism of HDL infusion that may contribute to reduction of sudden cardiac death.
authors.affiliation: Heart Failure Research Center, Academic Medical Center, University of Amsterdam, Meibergdreef 9, Amsterdam, the Netherlands.
authors.forename: Hester M
authors.initials: HM
authors.lastname: Den Ruijter
authors.forename: Remco
authors.initials: R
authors.lastname: Franssen
authors.forename: Arie O
authors.initials: AO
authors.lastname: Verkerk
authors.forename: Diederik F
authors.initials: DF
authors.lastname: van Wijk
authors.forename: Stefan F
authors.initials: SF
authors.lastname: Vaessen
authors.forename: Adriaan G
authors.initials: AG
authors.lastname: Holleboom
authors.forename: Johannes H
authors.initials: JH
authors.lastname: Levels
authors.forename: Tobias
authors.initials: T
authors.lastname: Opthof
authors.forename: Rattapong
authors.initials: R
authors.lastname: Sungnoon
authors.forename: Erik S
authors.initials: ES
authors.lastname: Stroes
authors.forename: Jan Albert
authors.initials: JA
authors.lastname: Kuivenhoven
authors.forename: Ruben
authors.initials: R
authors.lastname: Coronel
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D016632:Apolipoprotein A-I / Q000378:metabolism; D050197:Atherosclerosis / Q000378:metabolism; D016022:Case-Control Studies; D008076:Cholesterol, HDL / Q000378:metabolism*; D003327:Coronary Disease / Q000503:physiopathology*; D003645:Death, Sudden; D050171:Dyslipidemias / Q000378:metabolism; D004562:Electrocardiography / Q000379:methods; D005260:Female; D006321:Heart / Q000502:physiology*; D006329:Heart Conduction System / Q000503:physiopathology*; D006339:Heart Rate; D006801:Humans; D008297:Male; D008875:Middle Aged; D032383:Myocytes, Cardiac / Q000166:cytology*; D018408:Patch-Clamp Techniques; D011817:Rabbits; D014693:Ventricular Fibrillation / Q000378:metabolism
chemical_list: D016632:Apolipoprotein A-I; D008076:Cholesterol, HDL