title: Protocol for high-resolution electrophoresis separation of myosin heavy chain isoforms in bovine skeletal muscle.
abstract: In this short communication we describe a specific protocol for SDS-PAGE separation of adult bovine myosin heavy-chain (MyHC) isoforms. The conditions defined in this protocol allow a good separation with a good reproducibility of the four MyHC isoforms (MyHC I, IIa, IIx, IIb) identified in adult skeletal muscle of this species. This procedure uses mini-gel electrophoresis system and does not involve preparation of gradient separating gels. In addition, this protocol can also be applied to the electrophoretic separation of ovine and camel MyHC isoforms.
authors.affiliation: INRA, UR1213 Herbivores, Centre de Clermont-Ferrand/Theix, Saint Genès Champanelle, France. brigitte.picard@clermont.inra.fr
authors.forename: Brigitte
authors.initials: B
authors.lastname: Picard
authors.forename: Christiane
authors.initials: C
authors.lastname: Barboiron
authors.forename: David
authors.initials: D
authors.lastname: Chadeyron
authors.forename: Catherine
authors.initials: C
authors.lastname: Jurie
mesh_terms: D000818:Animals; D002162:Camelus; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006801:Humans; D018482:Muscle, Skeletal / Q000737:chemistry*; D018995:Myosin Heavy Chains / Q000737:chemistry / Q000302:isolation & purification*; D020033:Protein Isoforms; D051381:Rats; D015203:Reproducibility of Results; D012756:Sheep; D024744:Skeletal Muscle Myosins / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D020033:Protein Isoforms; D024744:Skeletal Muscle Myosins; D018995:Myosin Heavy Chains