title: Glycosynthase-mediated assembly of xylanase substrates and inhibitors.
abstract: An exo-β-xylosidase mutant with glycosynthase activity was created to aid in the synthesis of xylanase substrates and inhibitors. Simple monosaccharides were easily elaborated into di-, tri- and tetrasaccharides by using this enzyme. Some products proved to be surprisingly potent inhibitors of xylanases from glycoside hydrolase families 10 and 11.
authors.affiliation: Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver, British Columbia, V6T 1Z1 Canada.
authors.forename: Ethan D
authors.initials: ED
authors.lastname: Goddard-Borger
authors.forename: Brigitte
authors.initials: B
authors.lastname: Fiege
authors.forename: Emily M
authors.initials: EM
authors.lastname: Kwan
authors.forename: Stephen G
authors.initials: SG
authors.lastname: Withers
mesh_terms: D001407:Bacillus / Q000201:enzymology / Q000235:genetics; D004187:Disaccharides / Q000737:chemistry / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D014312:Trisaccharides / Q000737:chemistry / Q000378:metabolism; D014995:Xylosidases / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism*
chemical_list: D004187:Disaccharides; D014312:Trisaccharides; D006026:Glycoside Hydrolases; D014995:Xylosidases