title: Intact protein separation by chromatographic and/or electrophoretic techniques for top-down proteomics.
abstract: Mass spectrometry used in combination with a wide variety of separation methods is the principal methodology for proteomics. In bottom-up approach, proteins are cleaved with a specific proteolytic enzyme, followed by peptide separation and MS identification. In top-down approach intact proteins are introduced into the mass spectrometer. The ions generated by electrospray ionization are then subjected to gas-phase separation, fragmentation, fragment separation, and automated interpretation of mass spectrometric and chromatographic data yielding both the molecular weight of the intact protein and the protein fragmentation pattern. This approach requires high accuracy mass measurement analysers capable of separating the multi-charged isotopic cluster of proteins, such as hybrid ion trap-Fourier transform instruments (LTQ-FTICR, LTQ-Orbitrap). Front-end separation technologies tailored for proteins are of primary importance to implement top-down proteomics. This review intends to provide the state of art of protein chromatographic and electrophoretic separation methods suitable for MS coupling, and to illustrate both monodimensional and multidimensional approaches used for LC-MS top-down proteomics. In addition, some recent progresses in protein chromatography that may provide an alternative to those currently employed are also discussed.
authors.affiliation: Department of Chemistry, Sapienza Università di Roma, Piazzale Aldo Moro 5, 00185 Rome, Italy.
authors.forename: Anna Laura
authors.initials: AL
authors.lastname: Capriotti
authors.forename: Chiara
authors.initials: C
authors.lastname: Cavaliere
authors.forename: Patrizia
authors.initials: P
authors.lastname: Foglia
authors.forename: Roberto
authors.initials: R
authors.lastname: Samperi
authors.forename: Aldo
authors.initials: A
authors.lastname: Laganà
mesh_terms: D000818:Animals; D002417:Cattle; D002853:Chromatography, Liquid; D004586:Electrophoresis; D006801:Humans; D013058:Mass Spectrometry; D011506:Proteins / Q000302:isolation & purification*; D040901:Proteomics
chemical_list: D011506:Proteins