title: Allyl isothiocyanate (AITC) induces stomatal closure in Arabidopsis.
abstract: Isothiocyanates (ITCs) are degradation products of glucosinolates in crucifer plants and have repellent effect on insects, pathogens and herbivores. In this study, we report that exogenously applied allyl isothiocyanate (AITC) induced stomatal closure in Arabidopsis via production of reactive oxygen species (ROS) and nitric oxide (NO), and elevation of cytosolic Ca(2+) . AITC-induced stomatal closures were partially inhibited by an inhibitor of NADPH oxidase and completely inhibited by glutathione monoethyl ester (GSHmee). AITC-induced stomatal closure and ROS production were examined in abscisic acid (ABA) deficient mutant aba2-2 and methyl jasmonate (MeJA)-deficient mutant aos to elucidate involvement of endogenous ABA and MeJA. Genetic evidences have demonstrated that AITC-induced stomatal closure required MeJA priming but not ABA priming. These results raise the possibility that crucifer plants produce ITCs to induce stomatal closure, leading to suppression of water loss and invasion of fungi through stomata.
authors.affiliation: Graduate School of Natural Science and Technology, Okayama University, 1-1-1 Tsushima-naka, Okayama 700-8530, Japan.
authors.forename: Md Atiqur Rahman
authors.initials: MA
authors.lastname: Khokon
authors.forename: Md Sarwar
authors.initials: MS
authors.lastname: Jahan
authors.forename: Taniya
authors.initials: T
authors.lastname: Rahman
authors.forename: Mohammad Anowar
authors.initials: MA
authors.lastname: Hossain
authors.forename: Daichi
authors.initials: D
authors.lastname: Muroyama
authors.forename: Ikuko
authors.initials: I
authors.lastname: Minami
authors.forename: Shintaro
authors.initials: S
authors.lastname: Munemasa
authors.forename: Izumi C
authors.initials: IC
authors.lastname: Mori
authors.forename: Yoshimasa
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Murata
mesh_terms: D000085:Acetates / Q000494:pharmacology; D017360:Arabidopsis / Q000187:drug effects* / Q000502:physiology*; D002118:Calcium / Q000378:metabolism; D003517:Cyclopentanes / Q000494:pharmacology; D003593:Cytoplasm / Q000187:drug effects / Q000378:metabolism; D005978:Glutathione / Q000494:pharmacology; D017879:Isothiocyanates / Q000494:pharmacology*; D009068:Movement / Q000187:drug effects; D009154:Mutation / Q000235:genetics; D009569:Nitric Oxide / Q000378:metabolism; D054883:Oxylipins / Q000494:pharmacology; D054046:Plant Stomata / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D017879:Isothiocyanates; D054883:Oxylipins; D017382:Reactive Oxygen Species; D009569:Nitric Oxide; C072239:methyl jasmonate; C004471:allyl isothiocyanate; D005978:Glutathione; D002118:Calcium