title: Refolding your protein with a little help from REFOLD.
abstract: The expression and harvesting of proteins from insoluble inclusion bodies by solubilization and refolding is a technique commonly used in the production of recombinant proteins. Despite the importance of refolding, publications in the literature are essentially ad hoc reports consisting of a dazzling array of experimental protocols and a diverse collection of buffer cocktails. For the protein scientists, using this information to refold their protein of interest presents enormous challenges. Here, we describe some of the practical considerations in refolding and present several standard protocols. Further, we describe how refolding procedures can be designed and modified using the information in the REFOLD database (http://refold.med.monash.edu.au), a freely available, open repository for protocols describing the refolding and purification of recombinant proteins.
authors.affiliation: Department of Biochemistry and Molecular Biology, Monash University, Clayton, VIC, Australia.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Phan
authors.forename: Nasrin
authors.initials: N
authors.lastname: Yamout
authors.forename: Jason
authors.initials: J
authors.lastname: Schmidberger
authors.forename: Stephen P
authors.initials: SP
authors.lastname: Bottomley
authors.forename: Ashley M
authors.initials: AM
authors.lastname: Buckle
mesh_terms: D002021:Buffers; D002614:Chelating Agents / Q000737:chemistry; D002850:Chromatography, Gel; D030562:Databases, Protein; D003956:Dialysis; D004220:Disulfides / Q000737:chemistry; D002479:Inclusion Bodies / Q000737:chemistry / Q000235:genetics; D009532:Nickel / Q000737:chemistry; D015202:Protein Engineering; D058849:Protein Refolding; D011994:Recombinant Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D017386:Sequence Homology, Amino Acid; D012995:Solubility
chemical_list: D002021:Buffers; D002614:Chelating Agents; D004220:Disulfides; D011994:Recombinant Proteins; D009532:Nickel