title: [Biological change of normal human breast epithelial cells induced by B( a )P and NNK].
abstract: OBJECTIVE
  To explore the changes of biological characters of normal human epithelial cells treated chronicly with carcinogens.
  
  
  METHODS
  At picomolar concentrations of NNK and B [ a ] P carcinogens, noncancerous MCF10A cells were repeatedly treated. Properties of biological target endpoint were studied in cells after 20 cycle's treatment.
  
  
  RESULTS
  It was found that the treatment can induce cell acquisition of properties of reduced dependence on growth factors, anchorage-independent growth,and acinar-conformational disruption.
  
  
  CONCLUSIONS
  MCF10A cells can acquire cancer-related properties after long-term,low-concentration treatment with carcinogens.
authors.affiliation: Institute of Nutrition and Food Safety, Chinese CDC, Beijing 100050, China. xiaoyujoyce@gmail.com
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Song
authors.forename: Huaqian
authors.initials: H
authors.lastname: Wang
mesh_terms: D001564:Benzo(a)pyrene / Q000633:toxicity*; D001940:Breast / Q000166:cytology*; D002273:Carcinogens / Q000633:toxicity; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D004357:Drug Synergism; D004847:Epithelial Cells / Q000166:cytology*; D005260:Female; D006801:Humans; D009602:Nitrosamines / Q000633:toxicity*
chemical_list: D002273:Carcinogens; D009602:Nitrosamines; D001564:Benzo(a)pyrene; C016583:4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone