title: Design of a reversible biotin analog and applications in protein labeling, detection, and isolation.
abstract: To expand the applicability of the biotin-(strept)avidin system, a biotin analog with reversible binding under non-denaturing conditions has been designed, and its applications in protein labeling, detection, and isolation have been evaluated.
authors.affiliation: Life Technologies, 29851 Willow Creek Road, Eugene, OR 97402, USA. lai-qiang.ying@lifetech.com
authors.forename: Lai-Qiang
authors.initials: LQ
authors.lastname: Ying
authors.forename: Bruce P
authors.initials: BP
authors.lastname: Branchaud
mesh_terms: D001710:Biotin / Q000031:analogs & derivatives*; D045744:Cell Line, Tumor; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D006860:Hydrogen Bonding; D018613:Microscopy, Confocal; D011485:Protein Binding; D019809:Streptavidin / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D005456:Fluorescent Dyes; D001710:Biotin; D019809:Streptavidin