title: SUMO negatively regulates BACE expression.
abstract: OBJECTIVE
  The effect of SUMO on the promoter activity and mRNA expression of BACE gene was investigated to find the connection between sumoylation and APP processing.
  
  
  METHODS
  The BACE promoter activity was measured by reporter gene analysis and BACE mRNA level was investigated using real-time RT-PCR method.
  
  
  RESULTS
  BACE gene promoter activity was inhibited by the over-expression of SUMO proteins and was blocked by disrupting the SP1 site. Endogenous BACE mRNA level was also negatively regulated by the induction of SUMO proteins. Using a specific inhitor of SP1, BACE promoter activity was coordinately inhibited.
  
  
  CONCLUSIONS
  SUMO negatively regulates the BACE expression and SP1 is involved in the process.
authors.affiliation: School of Life Science, University of Science and Technology of China, Hefei, China. jnzhou@ustc.edu.cn
authors.forename: Hui
authors.initials: H
authors.lastname: Fang
authors.forename: Xin
authors.initials: X
authors.lastname: Du
authors.forename: Fan-Tao
authors.initials: FT
authors.lastname: Meng
authors.forename: Jiang-Ning
authors.initials: JN
authors.lastname: Zhou
mesh_terms: D053829:Amyloid Precursor Protein Secretases / Q000096:biosynthesis* / Q000235:genetics*; D016229:Amyloid beta-Peptides / Q000096:biosynthesis / Q000235:genetics; D016564:Amyloid beta-Protein Precursor / Q000096:biosynthesis / Q000235:genetics; D016282:Aspartic Acid Endopeptidases / Q000096:biosynthesis* / Q000235:genetics*; D002460:Cell Line; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D008156:Luciferases / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D025841:Small Ubiquitin-Related Modifier Proteins / Q000502:physiology*; D016329:Sp1 Transcription Factor / Q000378:metabolism; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: D016229:Amyloid beta-Peptides; D016564:Amyloid beta-Protein Precursor; D012333:RNA, Messenger; D025841:Small Ubiquitin-Related Modifier Proteins; D016329:Sp1 Transcription Factor; D004247:DNA; D008156:Luciferases; D053829:Amyloid Precursor Protein Secretases; D016282:Aspartic Acid Endopeptidases; C509280:BACE1 protein, human