title: Simultaneous determination of ginsenoside (G-Re, G-Rg1, G-Rg2, G-F1, G-Rh1) and protopanaxatriol in human plasma and urine by LC-MS/MS and its application in a pharmacokinetics study of G-Re in volunteers.
abstract: Ginsenoside Re (G-Re) improved the memory function of experimental animals in a preclinical study. Several types of saponins including G-Rg1, G-Rg2, G-F1, G-Rh1, and protopanaxatriol (PPT) may be the metabolites of G-Re according to reports from preclinical trials. In order to support a study of the pharmacokinetics of G-Re, an analytical method for G-Re and the co-detection of its probable metabolites using liquid chromatography tandem mass spectrometry (LC-MS/MS) was developed and validated. Solid phase extraction was utilized in the sample preparation. Separation of the analytes was achieved using a gradient elution (0.05% formic acid-methanol-acetonitrile, each organic phase containing 0.05% formic acid) at a flow rate of 0.3 mL/min with a retention time of approximately 2.88 min for G-Re. Data were acquired in the multiple reaction mode (MRM) and the linear range of the standard curve of plasma and urine samples for G-Re was 0.05-20 ng/mL with r(2)≥0.99. In the analysis of probable metabolites, G-Re, G-Rg1, G-F1, G-Rh1 and PPT were all detected in samples; however, G-Rg2 was not detected.
authors.affiliation: Department of Pharmacy, Beijing Hospital, The Ministry of Health, East-City District, Beijing, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.forename: Jianquan
authors.initials: J
authors.lastname: Huang
authors.forename: Xin
authors.initials: X
authors.lastname: Hu
authors.forename: Kexin
authors.initials: K
authors.lastname: Li
authors.forename: Chunhua
authors.initials: C
authors.lastname: Sun
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D004355:Drug Stability; D036145:Ginsenosides / Q000097:blood* / Q000378:metabolism / Q000493:pharmacokinetics / Q000652:urine*; D006801:Humans; D016014:Linear Models; D015203:Reproducibility of Results; D012502:Sapogenins / Q000097:blood* / Q000378:metabolism / Q000493:pharmacokinetics / Q000652:urine*; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D036145:Ginsenosides; D012502:Sapogenins; C081552:protopanaxatriol