title: Photoconversion mechanism of a green/red photosensory cyanobacteriochrome AnPixJ: time-resolved optical spectroscopy and FTIR analysis of the AnPixJ-GAF2 domain.
abstract: The photoconversion mechanism of a green/red sensory cyanobacteriochrome AnPixJ was studied. The phycocyanobilin-binding second GAF domain of AnPixJ of Anabaena sp. PCC 7120 was expressed in Escherichia coli cells. The His-tagged AnPixJ-GAF2 domain exhibited photoconversion between the green- and red-absorbing forms, APg(543) and APr(648), respectively. We detected four intermediate states in the photocycle between them, as follows: APr(648) → red light → APr(648)* → (with a rise time constant τ(r) of <100 ns) R1(650-80) (with a decay time constant τ(d) of <1 μs) → R2(610) (τ(d) = 920 μs) → APg(543) → green light → APg(543)* → (τ(r) < 50 ns) G1(570) (τ(d) = 190 μs) → G2(630) (τ(d) = 1.01 ms) → APr(648). These intermediates were named for their absorption peak wavelengths, which were estimated on the basis of the time-resolved difference spectra and global analysis of the time courses. The absorption spectrum of APr(648) resembles that of the Pr form of the phytochrome, while all the other states showed peaks at 530-650 nm and had wider bandwidths with smaller peak amplitudes. The fastest decay phases of fluorescence from APr(648)* and APg(543)* gave lifetimes of 200 and 42 ps, respectively, suggesting fast primary reactions. The APg(543)-minus-APr(648) difference FTIR spectrum in an H(2)O medium was significantly different from those reported for the Pfr/Pr difference spectra in phytochromes. Most of the peaks in the difference spectrum were shifted in the D(2)O medium, suggesting the high accessibility to the aqueous phase. The interactions of the phycocyanobilin chromophore with the surrounding amino acid residues, which are fairly different from those in the GAF domain of phytochromes, realize the unique green/red photocycle of AnPixJ.
authors.affiliation: Division of Material Science, Graduate School of Science, Nagoya University, Nagoya 464-8602, Japan.
authors.forename: Yoshimasa
authors.initials: Y
authors.lastname: Fukushima
authors.forename: Masayo
authors.initials: M
authors.lastname: Iwaki
authors.forename: Rei
authors.initials: R
authors.lastname: Narikawa
authors.forename: Masahiko
authors.initials: M
authors.lastname: Ikeuchi
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Tomita
authors.forename: Shigeru
authors.initials: S
authors.lastname: Itoh
mesh_terms: D000595:Amino Acid Sequence; D017033:Anabaena / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry*; D005453:Fluorescence; D008027:Light; D008969:Molecular Sequence Data; D055668:Photochemical Processes; D017434:Protein Structure, Tertiary; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013997:Time Factors
chemical_list: D001426:Bacterial Proteins