title: Discrimination between 5-hydroxymethylcytosine and 5-methylcytosine by a chemically designed peptide.
abstract: An artificial phosphopeptide recognized the difference between methylated and hydroxymethylated cytosines in DNA. The Sp1 zinc finger peptide substituted by phosphotyrosine effectively discriminated between 5-methylcytosine, 5-hydroxymethylcytosine ((hm)C) and unmethylated cytosine. The DNA recognition properties of the peptide differ from those of other chemicals that detect (hm)C.
authors.affiliation: Advanced Science Institute, RIKEN, Wako, Saitama 351-0198, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Nomura
authors.forename: Kaori
authors.initials: K
authors.lastname: Sugizaki
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Yanagisawa
authors.forename: Akimitsu
authors.initials: A
authors.lastname: Okamoto
mesh_terms: D044503:5-Methylcytosine / Q000032:analysis* / Q000031:analogs & derivatives; D003596:Cytosine / Q000031:analogs & derivatives* / Q000032:analysis; D004247:DNA / Q000737:chemistry; D024202:Electrophoretic Mobility Shift Assay / Q000379:methods*; D010084:Oxidation-Reduction; D010748:Phosphopeptides / Q000737:chemistry*; D019000:Phosphotyrosine / Q000737:chemistry; D011494:Protein Kinases / Q000737:chemistry; D016335:Zinc Fingers
chemical_list: D010748:Phosphopeptides; C011865:5-hydroxymethylcytosine; D019000:Phosphotyrosine; D044503:5-Methylcytosine; D003596:Cytosine; D004247:DNA; D011494:Protein Kinases; C065999:Sp1 kinase