title: Accumulation of long-chain glycosphingolipids during aging is prevented by caloric restriction.
abstract: BACKGROUND
  Chronic kidney disease and end-stage renal disease are major causes of morbidity and mortality that are seen far more commonly in the aged population. Interestingly, kidney function declines during aging even in the absence of underlying renal disease. Declining renal function has been associated with age-related cellular damage and dysfunction with reports of increased levels of apoptosis, necrosis, and inflammation in the aged kidney. Bioactive sphingolipids have been shown to regulate these same cellular processes, and have also been suggested to play a role in aging and cellular senescence.
  
  
  RESULTS
  We hypothesized that alterations in kidney sphingolipids play a role in the declining kidney function that occurs during aging. To begin to address this, the sphingolipid profile was measured in young (3 mo), middle aged (9 mo) and old (17 mo) C57BL/6 male mice. Interestingly, while modest changes in ceramides and sphingoid bases were evident in kidneys from older mice, the most dramatic elevations were seen in long-chain hexosylceramides (HexCer) and lactosylceramides (LacCer), with C14- and C16-lactosylceramides elevated as much as 8 and 12-fold, respectively. Increases in long-chain LacCers during aging are not exclusive to the kidney, as they also occur in the liver and brain. Importantly, caloric restriction, previously shown to prevent the declining kidney function seen in aging, inhibits accumulation of long-chain HexCer/LacCers and prevents the age-associated elevation of enzymes involved in their synthesis. Additionally, long-chain LacCers are also significantly elevated in human fibroblasts isolated from elderly individuals.
  
  
  CONCLUSIONS
  This study demonstrates accumulation of the glycosphingolipids HexCer and LacCer in several different organs in rodents and humans during aging. In addition, data demonstrate that HexCer and LacCer metabolism is regulated by caloric restriction. Taken together, data suggest that HexCer/LacCers are important mediators of cellular processes fundamental to mammalian aging.
authors.affiliation: Department of Medicine Medical University of South Carolina, Charleston, South Carolina, United States of America.
authors.forename: María José
authors.initials: MJ
authors.lastname: Hernández-Corbacho
authors.forename: Russell W
authors.initials: RW
authors.lastname: Jenkins
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Clarke
authors.forename: Yusuf A
authors.initials: YA
authors.lastname: Hannun
authors.forename: Lina M
authors.initials: LM
authors.lastname: Obeid
authors.forename: Ashley J
authors.initials: AJ
authors.lastname: Snider
authors.forename: Leah J
authors.initials: LJ
authors.lastname: Siskind
mesh_terms: D000369:Aged, 80 and over; D000375:Aging / Q000378:metabolism* / Q000502:physiology; D000818:Animals; D031204:Caloric Restriction; D002518:Ceramides / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D006028:Glycosphingolipids / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008875:Middle Aged; D009928:Organ Specificity; D010088:Oxidoreductases / Q000378:metabolism; D013108:Sphingomyelin Phosphodiesterase / Q000378:metabolism; D055815:Young Adult
chemical_list: D002518:Ceramides; D006028:Glycosphingolipids; D010088:Oxidoreductases; C108138:dihydroceramide desaturase; D013108:Sphingomyelin Phosphodiesterase