title: Myeloid cells migrate in response to IL-24.
abstract: IL-24 (melanoma differentiation associated gene 7 product) is a member of the IL-10 cytokine family that has been reported to possess anti-tumor activity. IL-24 is produced by immune tissues and its expression can be induced in human peripheral blood mononuclear cells by pathogen-associated molecules. While immune cells are known to produce IL-24, the response of immune cells to IL-24 is unclear. Using recombinant human IL-24, we demonstrated that IL-24 induces human monocyte and neutrophil migration, in vitro. An in vivo chemotaxis model showed that IL-24 attracted CD11b positive myeloid cells. To further characterize the chemotactic IL-24 response and type(s) of receptor(s) utilized by IL-24, we treated monocytes with signaling pathway inhibitors. IL-24-induced migration was reduced by pertussis toxin treatment, thus implicating G-protein coupled receptors in this process. Additionally, MEK and JAK inhibitors markedly decreased monocyte migration toward IL-24. These results suggest that IL-24 activates several signaling cascades in immune cells eliciting migration of myeloid cells, which may contribute to the known anti-cancer effects of IL-24.
authors.affiliation: Laboratory of Molecular Immunoregulation, Cancer and Inflammation Program, National Cancer Institute-Frederick, Center for Cancer Research, Frederick, MD 21702, USA.
authors.forename: Krisztina
authors.initials: K
authors.lastname: Buzas
authors.forename: Joost J
authors.initials: JJ
authors.lastname: Oppenheim
authors.forename: O M
authors.initials: OM
authors.lastname: Zack Howard
mesh_terms: D000818:Animals; D039481:CD11b Antigen / Q000096:biosynthesis; D002465:Cell Movement / Q000187:drug effects*; D002478:Cells, Cultured; D006801:Humans; D007378:Interleukins / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism; D020935:MAP Kinase Signaling System / Q000187:drug effects; D051379:Mice; D008810:Mice, Inbred C57BL; D022423:Myeloid Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism; D037342:Pertussis Toxin / Q000494:pharmacology; D011994:Recombinant Proteins / Q000494:pharmacology; D000099180:Interleukin-24
chemical_list: D039481:CD11b Antigen; D007378:Interleukins; D037342:Pertussis Toxin; D011994:Recombinant Proteins; D000099180:Interleukin-24