title: Surface wettability of plasma SiOx:H nanocoating-induced endothelial cells' migration and the associated FAK-Rho GTPases signalling pathways.
abstract: Vascular endothelial cell (EC) adhesion and migration are essential processes in re-endothelialization of implanted biomaterials. There is no clear relationship and mechanism between EC adhesion and migration behaviour on surfaces with varying wettabilities. As model substrates, plasma SiO(x):H nanocoatings with well-controlled surface wettability (with water contact angles in the range of 98.5 ± 2.3° to 26.3 ± 4.0°) were used in this study to investigate the effects of surface wettability on cell adhesion/migration and associated protein expressions in FAK-Rho GTPases signalling pathways. It was found that EC adhesion/migration showed opposite behaviour on the hydrophilic and hydrophobic surfaces (i.e. hydrophobic surfaces promoted EC migration but were anti-adhesions). The number of adherent ECs showed a maximum on hydrophilic surfaces, while cells adhered to hydrophobic surfaces exhibited a tendency for cell migration. The focal adhesion kinase (FAK) inhibitor targeting the Y-397 site of FAK could significantly inhibit cell adhesion/migration, suggesting that EC adhesion and migration on surfaces with different wettabilities involve (p)FAK and its downstream signalling pathways. Western blot results suggested that the FAK-Rho GTPases signalling pathways were correlative to EC migration on hydrophobic plasma SiO(x):H surfaces, but uncertain to hydrophilic surfaces. This work demonstrated that surface wettability could induce cellular behaviours that were associated with different cellular signalling events.
authors.affiliation: Institute of Biomedical Engineering, School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Shen
authors.forename: Guixue
authors.initials: G
authors.lastname: Wang
authors.forename: Xianliang
authors.initials: X
authors.lastname: Huang
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhang
authors.forename: Jiang
authors.initials: J
authors.lastname: Wu
authors.forename: Chaojun
authors.initials: C
authors.lastname: Tang
authors.forename: Qingsong
authors.initials: Q
authors.lastname: Yu
authors.forename: Xiaoheng
authors.initials: X
authors.lastname: Liu
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D002448:Cell Adhesion; D002465:Cell Movement / Q000187:drug effects*; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D051417:Focal Adhesion Kinase 1 / Q000378:metabolism*; D057927:Hydrophobic and Hydrophilic Interactions; D015398:Signal Transduction / Q000187:drug effects; D017655:Silicon Compounds / Q000737:chemistry / Q000494:pharmacology*; D013499:Surface Properties; D017155:Wettability; D020741:rho GTP-Binding Proteins / Q000378:metabolism*
chemical_list: D001672:Biocompatible Materials; D017655:Silicon Compounds; D051417:Focal Adhesion Kinase 1; C496050:PTK2 protein, human; D020741:rho GTP-Binding Proteins