title: Poly(β-amino ester)-DNA complexes: time-resolved fluorescence and cellular transfection studies.
abstract: A large number of different polymers have been developed and studied for application as DNA carriers for non-viral gene delivery, but the DNA binding properties are not understood. This study describes the efficiency of nanoparticle formation by time-resolved fluorescence measurements for poly(β-amino esters), cationic biodegradable polymers with DNA complexation and transfection capability. From the large library of poly(β-amino esters) ten polymers with different transfection efficacies were chosen for this study. The binding constants for nanoparticle formation were determined and compared to with the same method. Although the DNA binding efficiency of the amine groups are similar for both types of polymers, the overall binding constants are an order of magnitude smaller for poly(β-amino esters) than for 25 kDa polyethylenimines, yet poly(β-amino esters) show comparable DNA transfection efficacy with polyethylenimines. Within this series of polymers the transfection efficacy showed increasing trend in association with relative efficiency of nanoparticle formation.
authors.affiliation: Department of Chemistry and Bioengineering, Tampere University of Technology, P.O. Box 541, FIN-33101 Tampere, Finland.
authors.forename: Elina
authors.initials: E
authors.lastname: Vuorimaa
authors.forename: Tiia-Maaria
authors.initials: TM
authors.lastname: Ketola
authors.forename: Jordan J
authors.initials: JJ
authors.lastname: Green
authors.forename: Martina
authors.initials: M
authors.lastname: Hanzlíková
authors.forename: Helge
authors.initials: H
authors.lastname: Lemmetyinen
authors.forename: Robert
authors.initials: R
authors.lastname: Langer
authors.forename: Daniel G
authors.initials: DG
authors.lastname: Anderson
authors.forename: Arto
authors.initials: A
authors.lastname: Urtti
authors.forename: Marjo
authors.initials: M
authors.lastname: Yliperttula
mesh_terms: D004247:DNA / Q000235:genetics / Q000378:metabolism*; D053758:Nanoparticles; D011108:Polymers / Q000378:metabolism*; D011485:Protein Binding / Q000502:physiology; D013050:Spectrometry, Fluorescence / Q000379:methods; D013997:Time Factors; D014162:Transfection / Q000379:methods*
chemical_list: D011108:Polymers; C507253:poly(beta-amino ester); D004247:DNA