title: Bacterial community composition of biological degreasing systems and health risk assessment for workers.
abstract: Biological degreasing system is a new technology based on the degradation capabilities of microorganisms to remove oil, grease, or lubricants from metal parts. No data is available about the potential biological health hazards in such system. Thus, a health risk assessment linked to the bacterial populations present in this new degreasing technology is, therefore, necessary for workers. We performed both cultural and molecular approaches in several biological degreasing systems for various industrial contexts to investigate the composition and dynamics of bacterial populations. These biological degreasing systems did not work with the original bacterial populations. Indeed, they were colonized by a defined and restricted group of bacteria. This group replaced the indigenous bacterial populations known for degrading complex substrates. Klebsiella pneumoniae, Klebsiella oxytoca, Pseudomonas aeruginosa, and Pantoea agglomerans were important members of the microflora found in most of the biological degreasing systems. These bacteria might represent a potential health hazard for workers.
authors.affiliation: Clermont Université, Université d'Auvergne, Laboratoire: Microorganismes Génome et Environnement (LMGE), BP 10448, F-63000 Clermont-Ferrand, France.
authors.forename: Delphine
authors.initials: D
authors.lastname: Boucher
authors.forename: Jean Baptiste
authors.initials: JB
authors.lastname: Laffaire
authors.forename: Faouzi
authors.initials: F
authors.lastname: Jaziri
authors.forename: Christine
authors.initials: C
authors.lastname: David
authors.forename: Corinne
authors.initials: C
authors.lastname: Biderre-Petit
authors.forename: Philippe
authors.initials: P
authors.lastname: Duquenne
authors.forename: Eric
authors.initials: E
authors.lastname: Peyretaillade
authors.forename: Pierre
authors.initials: P
authors.lastname: Peyret
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification*; D015169:Colony Count, Microbial; D007218:Industrial Microbiology; D016273:Occupational Exposure; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D018570:Risk Assessment; D014865:Waste Disposal, Fluid / Q000379:methods
chemical_list: D012336:RNA, Ribosomal, 16S