title: The sap of Acer okamotoanum decreases serum alcohol levels after acute ethanol ingestion in rats.
abstract: In the present study, we examined whether Acer okamotoanum (A. okamotoanum) sap decreased the serum alcohol and acetaldehyde levels after acute ethanol treatment in a rat model. Male rats were orally administered 25, 50 or 100% A. okamotoanum sap 30 min prior to oral challenge with 3 ml of ethanol (15 ml/kg of a 20% ethanol solution in water), and the blood concentrations of alcohol and acetaldehyde were analyzed up to 7 h after the treatment. Pre-treatment with the sap significantly decreased the blood ethanol and acetaldehyde concentrations after 5 h when compared with ethanol treatment alone (a negative control). The expression levels of liver alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) mRNA were increased significantly in animals pre-treated with A. okamotoanum sap when compared with negative and positive controls. The data suggest that sap pre-treatment enhanced the alcohol metabolism rate in the rat liver. To investigate the involvement of mitochondrial regulation in the ethanol-induced hepatocyte apoptosis, we carried out an immunohistochemical analysis of Bax and Bcl-2. Pre-treatment with sap significantly decreased Bax expression and increased Bcl-2 expression 7 h after ethanol administration when compared with the negative control. The data suggest that A. okamotoanum sap pre-treatment may reduce the alcohol-induced oxidative stress in the rat liver.
authors.affiliation: Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk 361-763, Republic of Korea.
authors.forename: Yeong-Min
authors.initials: YM
authors.lastname: Yoo
authors.forename: Eui-Man
authors.initials: EM
authors.lastname: Jung
authors.forename: Ha-Young
authors.initials: HY
authors.lastname: Kang
authors.forename: In-Gyu
authors.initials: IG
authors.lastname: Choi
authors.forename: Kyung-Chul
authors.initials: KC
authors.lastname: Choi
authors.forename: Eui-Bae
authors.initials: EB
authors.lastname: Jeung
mesh_terms: D031002:Acer / Q000737:chemistry*; D000079:Acetaldehyde / Q000097:blood; D000426:Alcohol Dehydrogenase / Q000378:metabolism; D000818:Animals; D000431:Ethanol / Q000097:blood*; D007150:Immunohistochemistry; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D053147:Plant Exudates / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D053147:Plant Exudates; D000431:Ethanol; D000426:Alcohol Dehydrogenase; D000079:Acetaldehyde