title: In vivo characterization of tumor and tumor vascular network using multi-modal imaging approach.
abstract: We present a multi-modal optical diagnostic approach utilizing a combined use of Fluorescence Intravital Microscopy (FIM), Dynamic Light Scattering (DLS) and Spectrally Enhanced Microscopy (SEM) modalities for in vivo imaging of tumor vascular network and blood microcirculation. FIM is used for imaging of tumor surroundings and microenvironment, SEM provides information regarding blood vessels topography, whereas DLS is applied for functional imaging of vascular network and blood microcirculation. This complementary combination of the imaging approaches is extremely useful for functional in vivo imaging of blood vasculature and tumor microenvironment. The technique has also a great potential in vascular biology and can significantly expand the capabilities of tumor angiogenesis studies and notably contribute to the development of cancer treatment.
authors.affiliation: Department of Veterinary Resources, Weizmann Institute of Science, Rehovot, 76100, Israel. a.kalchenko@weizmann.ac.il
authors.forename: Vyacheslav
authors.initials: V
authors.lastname: Kalchenko
authors.forename: Noa
authors.initials: N
authors.lastname: Madar-Balakirski
authors.forename: Igor
authors.initials: I
authors.lastname: Meglinski
authors.forename: Alon
authors.initials: A
authors.lastname: Harmelin
mesh_terms: D000818:Animals; D003952:Diagnostic Imaging / Q000379:methods*; D005260:Female; D051379:Mice; D008819:Mice, Nude; D008833:Microcirculation; D008856:Microscopy, Fluorescence / Q000379:methods*; D018715:Microscopy, Video / Q000379:methods; D009374:Neoplasms, Experimental / Q000098:blood supply* / Q000473:pathology; D009389:Neovascularization, Pathologic / Q000175:diagnosis*; D012542:Scattering, Radiation; D019043:Vascular Neoplasms / Q000098:blood supply* / Q000473:pathology