title: The APC/C subunit 10 plays an essential role in cell proliferation during leaf development.
abstract: The largest E3 ubiquitin-ligase complex, known as anaphase-promoting complex/cyclosome (APC/C), regulates the proteolysis of cell cycle regulators such as CYCLIN B and SECURIN that are essential for sister-chromatid separation and exit from mitosis. Despite its importance, the role of APC/C in plant cells and the regulation of its activity during cell division remain poorly understood. Here, the Arabidopsis thaliana APC/C subunit APC10 was characterized and shown to functionally complement an apc10 yeast mutant. The APC10 protein was located in specific nuclear bodies, most probably resulting from its association with the proteasome complex. An apc10 Arabidopsis knockout mutant strongly impaired female gametogenesis. Surprisingly, constitutive overexpression of APC10 enhanced leaf size. Through kinematic analysis, the increased leaf size was found to be due to enhanced rates of cell division during the early stages of leaf development and, at the molecular level, by increased APC/C activity as measured by an amplification of the proteolysis rate of the mitotic cyclin, CYCB1;1.
authors.affiliation: Department of Plant Systems Biology, VIB, 9052 Gent, Belgium.
authors.forename: Nubia B
authors.initials: NB
authors.lastname: Eloy
authors.forename: Marcelo
authors.initials: M
authors.lastname: de Freitas Lima
authors.forename: Daniël
authors.initials: D
authors.lastname: Van Damme
authors.forename: Hannes
authors.initials: H
authors.lastname: Vanhaeren
authors.forename: Nathalie
authors.initials: N
authors.lastname: Gonzalez
authors.forename: Liesbeth
authors.initials: L
authors.lastname: De Milde
authors.forename: Adriana S
authors.initials: AS
authors.lastname: Hemerly
authors.forename: Gerrit T S
authors.initials: GT
authors.lastname: Beemster
authors.forename: Dirk
authors.initials: D
authors.lastname: Inzé
authors.forename: Paulo C G
authors.initials: PC
authors.lastname: Ferreira
mesh_terms: D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D001696:Biomechanical Phenomena; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D049109:Cell Proliferation; D019926:Cyclin B / Q000235:genetics / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D056130:Gametogenesis, Plant / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D005816:Genetic Complementation Test; D005838:Genotype; D005966:Glucuronidase; D049452:Green Fluorescent Proteins; D009154:Mutation; D010641:Phenotype; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics / Q000254:growth & development*; D030821:Plants, Genetically Modified; D059748:Proteolysis; D018749:RNA, Plant / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D013997:Time Factors; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D018797:Cell Cycle Proteins; C462528:CycB1 protein, Arabidopsis; D019926:Cyclin B; D018076:DNA, Complementary; D018749:RNA, Plant; C586858:anaphase-promoting complex subunit 10 protein, Arabidopsis; D049452:Green Fluorescent Proteins; D044767:Ubiquitin-Protein Ligases; D005966:Glucuronidase