title: Dynamic regulation of circulating microRNA during acute exhaustive exercise and sustained aerobic exercise training.
abstract: MicroRNAs (miRNAs) are intracellular mediators of essential biological functions. Recently, plasma-based 'circulating' miRNAs (c-miRNAs) have been shown to control cellular processes, but the c-miRNA response to human exercise remains unknown. We sought to determine whether c-miRNAs are dynamically regulated in response to acute exhaustive cycling exercise and sustained rowing exercise training using a longitudinal, repeated measures study design. Specifically, c-miRNAs involved in angiogenesis (miR-20a, miR-210, miR-221, miR-222, miR-328), inflammation (miR-21, miR-146a), skeletal and cardiac muscle contractility (miR-21, miR-133a), and hypoxia/ischaemia adaptation (miR-21, miR-146a, and miR-210) were measured at rest and immediately following acute exhaustive cycling exercise in competitive male rowers (n = 10, age = 19.1 ± 0.6 years) before and after a 90 day period of rowing training. Distinct patterns of c-miRNA response to exercise were observed and adhered to four major profiles: (1) c-miRNA up-regulated by acute exercise before and after sustained training (miR-146a and miR-222), (2) c-miRNA responsive to acute exercise before but not after sustained training (miR-21 and miR-221), (3) c-miRNA responsive only to sustained training (miR-20a), and (4) non-responsive c-miRNA (miR-133a, miR-210, miR-328). Linear correlations were observed between peak exercise levels of miR-146a and VO2max (r = 0.63, P = 0.003) and between changes in resting miR-20a and changes in VO2max (pre-training vs. post-training, r = 0.73; P = 0.02). Although future work is required, these results suggest the potential value of c-miRNAs as exercise biomarkers and their possible roles as physiological mediators of exercise-induced cardiovascular adaptation.
authors.affiliation: Massachusetts General Hospital, Department of Medicine, Harvard Medical School, Boston, MA 02114, USA. abaggish@partners.org
authors.forename: Aaron L
authors.initials: AL
authors.lastname: Baggish
authors.forename: Andrew
authors.initials: A
authors.lastname: Hale
authors.forename: Rory B
authors.initials: RB
authors.lastname: Weiner
authors.forename: Gregory D
authors.initials: GD
authors.lastname: Lewis
authors.forename: David
authors.initials: D
authors.lastname: Systrom
authors.forename: Francis
authors.initials: F
authors.lastname: Wang
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Wang
authors.forename: Stephen Y
authors.initials: SY
authors.lastname: Chan
mesh_terms: D056352:Athletes; D015331:Cohort Studies; D015444:Exercise / Q000502:physiology*; D006801:Humans; D008137:Longitudinal Studies; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000502:physiology; D010807:Physical Endurance / Q000502:physiology*; D011446:Prospective Studies; D013997:Time Factors; D055815:Young Adult
chemical_list: D035683:MicroRNAs