title: [Correlation of band 3 protein in erythrocytes with physiological status of middle-aged and elderly people].
abstract: OBJECTIVE
  To analyze the correlation of band 3 protein of erythrocytes with physiological conditions of middle-aged and elderly people, and to investigate the expression of band 3 protein under different physiological status.
  
  
  METHODS
  Cluster randomized sampling method for selecting subjects . Physical examination data and blood samples of 218 middle-aged and elderly people (80 males and 138 females) were collected. SDS-polyacrylamide gel electrophoresis was used to analyze band 3 protein in erythrocyte membrane. The difference of band 3 protein in different groups was analyzed by T Test and One-Way ANOVA. Correlation and regression of band 3 protein with physiological status was analyzed by Pearson correlation and linear regression.
  
  
  RESULTS
  There was a significantly positive correlation (r =0.149) between the level of band 3 protein and physical activity (P <0.05), and a negative correlation (r = -0.156) between the level of band 3 protein and systolic blood pressure (P <0.05). The correlation of band 3 protein with gender, age, BMI and diastolic blood pressure (P >0.05) is not significant. The band 3 protein level of workers with medium activity was 24. 09% , which was significantly higher than that of light activity workers (23.42%) (P <0.05). The subjects with hypertension were proved to have a significantly lower level of band 3 protein (23.33%) than normal individuals (24.20%) (P < 0.05).
  
  
  CONCLUSIONS
  The level of band 3 protein was positively correlated with physical activity and negatively correlated with systolic blood pressure.
authors.affiliation: Institute of Nutrition Research, Qingdao University Medical College, Qingdao 266021, China.
authors.forename: Wenlu
authors.initials: W
authors.lastname: Chang
authors.forename: Yongye
authors.initials: Y
authors.lastname: Sun
authors.forename: Qiuzhen
authors.initials: Q
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Du
authors.forename: Xueqian
authors.initials: X
authors.lastname: Wu
authors.forename: Aigou
authors.initials: A
authors.lastname: Ma
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001457:Anion Exchange Protein 1, Erythrocyte / Q000502:physiology*; D001794:Blood Pressure; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009043:Motor Activity; D010829:Physiological Phenomena; D012494:Sampling Studies; D011795:Surveys and Questionnaires
chemical_list: D001457:Anion Exchange Protein 1, Erythrocyte