title: Controlling specific locomotor behaviors through multidimensional monoaminergic modulation of spinal circuitries.
abstract: Descending monoaminergic inputs markedly influence spinal locomotor circuits, but the functional relationships between specific receptors and the control of walking behavior remain poorly understood. To identify these interactions, we manipulated serotonergic, dopaminergic, and noradrenergic neural pathways pharmacologically during locomotion enabled by electrical spinal cord stimulation in adult spinal rats in vivo. Using advanced neurobiomechanical recordings and multidimensional statistical procedures, we reveal that each monoaminergic receptor modulates a broad but distinct spectrum of kinematic, kinetic, and EMG characteristics, which we expressed into receptor-specific functional maps. We then exploited this catalog of monoaminergic tuning functions to devise optimal pharmacological combinations to encourage locomotion in paralyzed rats. We found that, in most cases, receptor-specific modulatory influences summed near algebraically when stimulating multiple pathways concurrently. Capitalizing on these predictive interactions, we elaborated a multidimensional monoaminergic intervention that restored coordinated hindlimb locomotion with normal levels of weight bearing and partial equilibrium maintenance in spinal rats. These findings provide new perspectives on the functions of and interactions between spinal monoaminergic receptor systems in producing stepping, and define a framework to tailor pharmacotherapies for improving neurological functions after CNS disorders.
authors.affiliation: Neurology Department, University of Zurich, CH-8008 Zurich, Switzerland.
authors.forename: Pavel
authors.initials: P
authors.lastname: Musienko
authors.forename: Rubia
authors.initials: R
authors.lastname: van den Brand
authors.forename: Olivia
authors.initials: O
authors.lastname: Märzendorfer
authors.forename: Roland R
authors.initials: RR
authors.lastname: Roy
authors.forename: Yury
authors.initials: Y
authors.lastname: Gerasimenko
authors.forename: V Reggie
authors.initials: VR
authors.lastname: Edgerton
authors.forename: Grégoire
authors.initials: G
authors.lastname: Courtine
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000502:physiology*; D015306:Biogenic Monoamines / Q000378:metabolism*; D005260:Female; D005684:Gait / Q000502:physiology*; D008124:Locomotion / Q000502:physiology*; D009415:Nerve Net / Q000502:physiology*; D009434:Neural Pathways / Q000502:physiology; D009473:Neuronal Plasticity / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000502:physiology*
chemical_list: D015306:Biogenic Monoamines