title: [Expression analysis of 5 hsp70 genes in Tetrahymena thermophila].
abstract: Thirteen hsp70 genes with complete conserved domains were identified in Tetrahymena thermophila, and expression of five similar and non-intron hsp70 genes were analyzed. Under heat shock conditions of 37, 39 and 41oC, hsp70-2 mRNA had the highest relative expression level, suggesting it is closely related to heat shock. The basal level of constitutive T. thermophila hsp70-4 gene was high during 20 physiological/developmental stages of growth, starvation and conjugation, and it changed little upon exposure to heat shock: evidence that hsp70-4 is an hsc70 gene. The hsp70-4 cDNA is 2 208 bp long, and contains an open reading frame of 1 959 bp encoding 635 amino acids. Microarray data indicated that T. thermophila hsp70-3 gene probably participated in early starvation (0-12 h) stress and late conjugation (6-10 h) events, such as new macronuclear and micronuclear anlagen formation and old macronuclear elimination. However, hsp70-5 gene possibly participates in late starvation (12-15 h) stress and early conjugation (0-6 h) events such as micronuclear meiosis, micronuclear exchange and pronuclear fusion. Blast2GO indicated that they participated in dissimilar biological processes, suggesting hsp70-3 and hsp70-5 perform different functions.
authors.affiliation: Key Laboratory of Aquatic Biodiversity and Conservation, Institute of Hydrobiology, the Chinese Academy of Sciences, Wuhan 430072, China.
authors.forename: Li-Fang
authors.initials: LF
authors.lastname: Feng
authors.forename: Yue
authors.initials: Y
authors.lastname: Chang
authors.forename: Dong-Xia
authors.initials: DX
authors.lastname: Yuan
authors.forename: Wei
authors.initials: W
authors.lastname: Miao
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D018507:Gene Expression Regulation, Developmental; D018840:HSP70 Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D018869:Heat-Shock Response; D008969:Molecular Sequence Data; D005810:Multigene Family; D010802:Phylogeny; D015800:Protozoan Proteins / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment; D016808:Tetrahymena thermophila / Q000145:classification / Q000235:genetics* / Q000254:growth & development / Q000502:physiology
chemical_list: D018840:HSP70 Heat-Shock Proteins; D015800:Protozoan Proteins