title: Live cell imaging reveals structural associations between the actin and microtubule cytoskeleton in Arabidopsis.
abstract: In eukaryotic cells, the actin and microtubule (MT) cytoskeletal networks are dynamic structures that organize intracellular processes and facilitate their rapid reorganization. In plant cells, actin filaments (AFs) and MTs are essential for cell growth and morphogenesis. However, dynamic interactions between these two essential components in live cells have not been explored. Here, we use spinning-disc confocal microscopy to dissect interaction and cooperation between cortical AFs and MTs in Arabidopsis thaliana, utilizing fluorescent reporter constructs for both components. Quantitative analyses revealed altered AF dynamics associated with the positions and orientations of cortical MTs. Reorganization and reassembly of the AF array was dependent on the MTs following drug-induced depolymerization, whereby short AFs initially appeared colocalized with MTs, and displayed motility along MTs. We also observed that light-induced reorganization of MTs occurred in concert with changes in AF behavior. Our results indicate dynamic interaction between the cortical actin and MT cytoskeletons in interphase plant cells.
authors.affiliation: Max Planck Institute of Molecular Plant Physiology, 14476 Potsdam, Germany.
authors.forename: Arun
authors.initials: A
authors.lastname: Sampathkumar
authors.forename: Jelmer J
authors.initials: JJ
authors.lastname: Lindeboom
authors.forename: Seth
authors.initials: S
authors.lastname: Debolt
authors.forename: Ryan
authors.initials: R
authors.lastname: Gutierrez
authors.forename: David W
authors.initials: DW
authors.lastname: Ehrhardt
authors.forename: Tijs
authors.initials: T
authors.lastname: Ketelaar
authors.forename: Staffan
authors.initials: S
authors.lastname: Persson
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism* / Q000648:ultrastructure; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D017360:Arabidopsis / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure*; D047630:Depsipeptides / Q000494:pharmacology; D008870:Microtubules / Q000378:metabolism* / Q000648:ultrastructure; D059828:Plant Cells / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D053778:Thiazolidines / Q000494:pharmacology
chemical_list: D000970:Antineoplastic Agents; D019086:Bridged Bicyclo Compounds, Heterocyclic; D047630:Depsipeptides; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D053778:Thiazolidines; C057531:jasplakinolide; C037068:latrunculin B