title: Investigations on antibody binding to a micro-cantilever coated with a BAM pesticide residue.
abstract: The attachment of an antibody to an antigen-coated cantilever has been investigated by repeated experiments, using a cantilever-based detection system by Cantion A/S. The stress induced by the binding of a pesticide residue BAM (2,6 dichlorobenzamide) immobilized on a cantilever surface to anti-BAM antibody is measured using the CantiLab4© system from Cantion A/S with four gold-coated cantilevers and piezo resistive readout. The detection mechanism is in principle label-free, but fluorescent-marked antibodies have been used to subsequently verify the binding on the cantilever surface. The bending and increase in mass of each cantilever has also been investigated using a light interferometer and a Doppler Vibrometer. The system has been analyzed during repeated measurements to investigate whether the CantiLab4© system is a suited platform for a pesticide assay system.
authors.affiliation: Department of Micro- and Nanotechnology, Technical University of Denmark, DTU Nanotech, Building 345 East, 2800 Kongens Lyngby, Denmark. Michael.bache@nanotech.dtu.dk.
authors.forename: Michael
authors.initials: M
authors.lastname: Bache
authors.forename: Rafael
authors.initials: R
authors.lastname: Taboryski
authors.forename: Silvan
authors.initials: S
authors.lastname: Schmid
authors.forename: Jens
authors.initials: J
authors.lastname: Aamand
authors.forename: Mogens Havsteen
authors.initials: MH
authors.lastname: Jakobsen