title: On the potential involvement of CD11d in co-stimulating the production of interferon-γ by natural killer cells upon interaction with neutrophils via intercellular adhesion molecule-3.
abstract: Interaction between neutrophils and other leukocytes plays a variety of important roles in regulating innate and adaptive immune responses. Recently, we have shown that neu-trophils amplify NK cell/6-sulfo LacNAc(+) dendritic cells (slanDC)-mediated cytokine production, by potentiating IL-12p70 release by slanDC via CD18/ICAM-1 and directly co-stimulating IFNγ production by NK cells via ICAM-3. Herein, we have identified additional molecules involved in the interactions among neutrophils, NK cells and slanDC. More specifically, we provide evidence that: i) the cross-talk between neutrophils and NK cells is mediated by ICAM-3 and CD11d/CD18, respectively; ii) slanDC potentiate the production of IFNγ by NK cells via CD11a/CD18. Altogether, our studies shed more light on the role that adhesion molecules play within the neutrophil/NK cell/slanDC network. Our data also have potential implications in the pathogenesis of diseases driven by hyperactivated leukocytes, such as Sweet's syndrome, in which a neutrophil/NK cell co-localization is frequently observed.
authors.affiliation: Department of Pathology and Diagnostics, Division of General Pathology, University of Verona, Verona, Italy.
authors.forename: Claudio
authors.initials: C
authors.lastname: Costantini
authors.forename: Alessandra
authors.initials: A
authors.lastname: Micheletti
authors.forename: Federica
authors.initials: F
authors.lastname: Calzetti
authors.forename: Omar
authors.initials: O
authors.lastname: Perbellini
authors.forename: Nicola
authors.initials: N
authors.lastname: Tamassia
authors.forename: Cristina
authors.initials: C
authors.lastname: Albanesi
authors.forename: William
authors.initials: W
authors.lastname: Vermi
authors.forename: Marco A
authors.initials: MA
authors.lastname: Cassatella
mesh_terms: D015703:Antigens, CD / Q000378:metabolism*; D018845:CD11 Antigens / Q000378:metabolism*; D040881:CD11a Antigen / Q000378:metabolism; D018821:CD18 Antigens / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002450:Cell Communication; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism; D006801:Humans; D039001:Integrin alpha Chains / Q000378:metabolism*; D007371:Interferon-gamma / Q000096:biosynthesis*; D018664:Interleukin-12 / Q000096:biosynthesis; D007694:Killer Cells, Natural / Q000276:immunology* / Q000378:metabolism; D037181:Lectins, C-Type / Q000378:metabolism; D009504:Neutrophils / Q000276:immunology* / Q000378:metabolism; D011485:Protein Binding; D021381:Protein Transport; D011956:Receptors, Cell Surface / Q000378:metabolism; D016463:Sweet Syndrome / Q000276:immunology / Q000378:metabolism; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
chemical_list: D015703:Antigens, CD; D018845:CD11 Antigens; D040881:CD11a Antigen; D018821:CD18 Antigens; D015815:Cell Adhesion Molecules; D039001:Integrin alpha Chains; D007371:Interferon-gamma; D018664:Interleukin-12; D037181:Lectins, C-Type; D011956:Receptors, Cell Surface; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin; C483616:ICAM3 protein, human; C406812:ITGAD protein, human