title: Rat hippocampal proteomic alterations following intrahippocampal injection of amyloid beta peptide (1-40).
abstract: Amyloid beta peptide 1-40 (Aβ(1-40)) is closely associated with the progressive neuronal loss and cognitive decline observed in Alzheimer's disease (AD). This study aimed to establish a proteomic strategy for the profiling of AD tissues for disease-specific changes in protein abundance. Intrahippocampal injection of Aβ(1-40) induced spatial memory and learning decline in rats. Proteomic analysis revealed the changes in protein expression in the rat hippocampus treated with Aβ(1-40). Four proteins of interest which was in abundance was significantly altered in Aβ(1-40)-treated rats were identified by peptide mass fingerprint (PMF). These proteins corresponded to synapsin Ib, protein disulfide-isomerase A3 precursor, tubulin β chain and ATP synthase β subunit. Our results provide new insights into the relationship between Aβ and the pathogenesis of AD, and suggest potential targets for the therapy of AD.
authors.affiliation: Department of Traditional Chinese Medicine, The Second Affiliated Hospital, Dalian Medical University, Dalian 116023, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Shi
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Lu
authors.forename: Libin
authors.initials: L
authors.lastname: Zhan
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.forename: Mingzhong
authors.initials: M
authors.lastname: Sun
authors.forename: Xiaoyang
authors.initials: X
authors.lastname: Gong
authors.forename: Hua
authors.initials: H
authors.lastname: Sui
authors.forename: Xinping
authors.initials: X
authors.lastname: Niu
authors.forename: Shuqing
authors.initials: S
authors.lastname: Liu
authors.forename: Luping
authors.initials: L
authors.lastname: Zheng
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D016229:Amyloid beta-Peptides / Q000494:pharmacology*; D000818:Animals; D001362:Avoidance Learning / Q000187:drug effects; D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D008568:Memory / Q000187:drug effects; D010446:Peptide Fragments / Q000494:pharmacology*; D020543:Proteome / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; D020543:Proteome; C078592:amyloid beta-protein (1-40)