title: Preparation of anti-CD40 antibody modified magnetic PCL-PEG-PCL microspheres.
abstract: Antibody modified magnetic polymeric microspheres have potential biomedical application. In this paper, anti-CD40 antibody modified magnetic poly(epsilon-caprolactone)-poly(ethylene glycol)-poly(epsilon-caprolactone) (PCL-PEG-PCL, PCEC) microspheres were prepared. First, PCL-PEG-PCL triblock copolymer was synthesized by ring-opening polymerization, followed by reaction with succinic anhydride, creating carboxylated PCL-PEG-PCL copolymer. Then, magnetite nanoparticles were encapsulated into carboxylated PCL-PEG-PCL microspheres, forming magnetic PCL-PEG-PCL microspheres with carboxyl group on their surface. Catalyzed by EDC/NHS, the anti-CD40 antibody was linked to these magnetic PCL-PEG-PCL microspheres, thus forming anti-CD40 modified PCL-PEG-PCL microspheres. These anti-CD40 antibody modified magnetic PCL-PEG-PCL microspheres may have potential application in cell separation.
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, P R. China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Gao
authors.forename: Bing
authors.initials: B
authors.lastname: Kan
authors.forename: MaLing
authors.initials: M
authors.lastname: Gou
authors.forename: Juan
authors.initials: J
authors.lastname: Zhang
authors.forename: Gang
authors.initials: G
authors.lastname: Guo
authors.forename: Ning
authors.initials: N
authors.lastname: Huang
authors.forename: Xia
authors.initials: X
authors.lastname: Zhao
authors.forename: ZhiYong
authors.initials: Z
authors.lastname: Qian
mesh_terms: D000906:Antibodies / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D019013:CD40 Antigens / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D002469:Cell Separation; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D008863:Microspheres; D036103:Nanotechnology; D019906:Nuclear Magnetic Resonance, Biomolecular; D011091:Polyesters / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*
chemical_list: D000906:Antibodies; D019013:CD40 Antigens; D058185:Magnetite Nanoparticles; D011091:Polyesters; C515340:poly(epsilon-caprolactone)-b-poly(ethyleneglycol)-b-poly(epsilon-caprolactone); D011092:Polyethylene Glycols