title: A simple test for the detection of KPC and metallo-β-lactamase carbapenemase-producing Pseudomonas aeruginosa isolates with the use of meropenem disks supplemented with aminophenylboronic acid, dipicolinic acid and cloxacillin.
abstract: We evaluated the ability of the combination disk test (CDT) and the Modified Hodge Test (MHT) to discriminate between various carbapenemase-producing Pseudomonas aeruginosa isolates (KPC, n = 36; metallo-β-lactamase (MBL), n = 38) and carbapenemase non-producers (n = 75). For the CDT, the optimal inhibitor concentrations and cut-off values were: 600 μg of 3-aminophenylboronic acid (APB) per disk (an increment of ≥4 mm), 1000 μg of dipicolinic acid (DPA) per disk (an increment of ≥5 mm) and 3000 μg of cloxacillin per disk (an increment of ≥3 mm). APB had excellent sensitivity (97%) and specificity (97%) for the detection of KPC enzymes. DPA detected MBL enzymes with a sensitivity and specificity of 97% and 81%, respectively. The MHT resulted in a low sensitivity (78%) and specificity (57%). The CDT could be very useful in daily practice to provide fast and reliable detection of KPC and MBL carbapenemases among P. aeruginosa isolates.
authors.affiliation: Servicio Antimicrobianos, Departamento Bacteriología, Instituto Nacional de Enfermedades Infecciosas (INEI) - ANLIS 'Dr Carlos G. Malbrán', Ministerio de Salud y Ambiente, Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: F
authors.initials: F
authors.lastname: Pasteran
authors.forename: O
authors.initials: O
authors.lastname: Veliz
authors.forename: D
authors.initials: D
authors.lastname: Faccone
authors.forename: L
authors.initials: L
authors.lastname: Guerriero
authors.forename: M
authors.initials: M
authors.lastname: Rapoport
authors.forename: T
authors.initials: T
authors.lastname: Mendez
authors.forename: A
authors.initials: A
authors.lastname: Corso
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000187:drug effects / Q000378:metabolism*; D001897:Boronic Acids / Q000494:pharmacology; D003023:Cloxacillin / Q000494:pharmacology; D052978:Disk Diffusion Antimicrobial Tests / Q000379:methods*; D006801:Humans; D000077731:Meropenem; D010848:Picolinic Acids / Q000494:pharmacology; D011552:Pseudomonas Infections / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000187:drug effects / Q000201:enzymology*; D012680:Sensitivity and Specificity; D013845:Thienamycins / Q000494:pharmacology*; D001618:beta-Lactamases / Q000187:drug effects / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D001897:Boronic Acids; D010848:Picolinic Acids; D013845:Thienamycins; C028592:3-aminobenzeneboronic acid; D001618:beta-Lactamases; C063912:carbapenemase; D000077731:Meropenem; D003023:Cloxacillin; C004860:dipicolinic acid