title: Nanoscopy in a living multicellular organism expressing GFP.
abstract: We report superresolution fluorescence microscopy in an intact living organism, namely Caenorhabditis elegans nematodes expressing green fluorescent protein (GFP)-fusion proteins. We also superresolve, by stimulated emission depletion (STED) microscopy, living cultured cells, demonstrating that STED microscopy with GFP can be widely applied. STED with GFP can be performed with both pulsed and continuous-wave lasers spanning a wide wavelength range from at least 556-592 nm. Acquiring subdiffraction resolution images within seconds enables the recording of movies revealing structural dynamics. These results demonstrate that numerous microscopy studies of live samples employing GFP as the marker can be performed at subdiffraction resolution.
authors.affiliation: Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
authors.forename: Brian R
authors.initials: BR
authors.lastname: Rankin
authors.forename: Gael
authors.initials: G
authors.lastname: Moneron
authors.forename: Christian A
authors.initials: CA
authors.lastname: Wurm
authors.forename: Jessica C
authors.initials: JC
authors.lastname: Nelson
authors.forename: Arne
authors.initials: A
authors.lastname: Walter
authors.forename: Dirk
authors.initials: D
authors.lastname: Schwarzer
authors.forename: Jörg
authors.initials: J
authors.lastname: Schroeder
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Colón-Ramos
authors.forename: Stefan W
authors.initials: SW
authors.lastname: Hell
mesh_terms: D000042:Absorption; D000818:Animals; D017173:Caenorhabditis elegans / Q000166:cytology / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000378:metabolism*; D008856:Microscopy, Fluorescence / Q000379:methods*; D036103:Nanotechnology / Q000379:methods*; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000378:metabolism*; D013057:Spectrum Analysis
chemical_list: D049452:Green Fluorescent Proteins