title: Genomics and molecular genetics of Clonorchis sinensis: current status and perspectives.
abstract: Clonorchiasis caused by Clonorchis sinensis is an important foodborne parasitosis of humans and animals, and is predominantly a hepatobiliary disease. Globally, nearly 35 million people were infected with C. sinensis, with approximately 15 million being in China. Patients would chronically present fatigue, jaundice, abdominal discomfort, along with the increased risk of developing into a form of cholangiocarcinoma that is fatal to humans. Treatment of clonorchiasis by praziquantel has been very successful, but this is dependent on early accurate diagnosis and correct species identification. The present article reviews the current status of knowledge in genomics and functional genomics of C. sinensis, and summarizes the main DNA-based techniques for the specific diagnosis of C. sinensis infection and studies of genetic variation in C. sinensis, and provides perspectives for future studies. The advances in genomics and molecular genetics of C. sinensis shed new sight on our understanding of population structure of C. sinensis as well as the prevention and control of clonorchiasis.
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou, Gansu Province 730046, China.
authors.forename: Si-Yang
authors.initials: SY
authors.lastname: Huang
authors.forename: Guang-Hui
authors.initials: GH
authors.lastname: Zhao
authors.forename: Bao-Quan
authors.initials: BQ
authors.lastname: Fu
authors.forename: Min-Jun
authors.initials: MJ
authors.lastname: Xu
authors.forename: Chun-Ren
authors.initials: CR
authors.lastname: Wang
authors.forename: Song-Ming
authors.initials: SM
authors.lastname: Wu
authors.forename: Feng-Cai
authors.initials: FC
authors.lastname: Zou
authors.forename: Xing-Quan
authors.initials: XQ
authors.lastname: Zhu
mesh_terms: D000818:Animals; D003003:Clonorchiasis / Q000175:diagnosis* / Q000453:epidemiology / Q000469:parasitology / Q000517:prevention & control; D003004:Clonorchis sinensis / Q000235:genetics*; D020224:Expressed Sequence Tags; D005393:Fish Diseases / Q000175:diagnosis / Q000453:epidemiology; D005399:Fishes / Q000469:parasitology; D014644:Genetic Variation; D049751:Genome, Helminth; D006801:Humans; D035683:MicroRNAs / Q000032:analysis; D058977:Molecular Sequence Annotation / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods* / Q000662:veterinary; D009696:Nucleic Acids / Q000032:analysis; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000662:veterinary; D012908:Snails / Q000469:parasitology; D059467:Transcriptome
chemical_list: D035683:MicroRNAs; D009696:Nucleic Acids