title: Sulfite determination by an inhibitor biosensor-based mushroom (Agaricus bisporus) tissue homogenate.
abstract: The aim of the study presented here is to develop a biosensor based on mushroom (Agaricus bisporus) tissue homogenate for sensitive and economical determination of sulfite in foods. The working principle of the biosensor is based on an inhibition effect of sulfite on polyphenol oxidases in mushroom. Mushroom tissue homogenate was immobilized by gelatin and glutaraldehyde on a Clark-type oxygen electrode. Some optimization studies related to the bioactive layer components and working conditions were identified. The biosensor was applied to the food samples. The biosensor reported here was successfully allowed to analyze sulfite, which was a food additive in real food samples.
authors.affiliation: Namık Kemal University, Faculty of Arts and Sciences, Chemistry Department, Biochemistry Division, Tekirdağ, Turkey. msezginturk@hotmail.com
authors.forename: Mustafa Kemal
authors.initials: MK
authors.lastname: Sezgintürk
authors.forename: Erhan
authors.initials: E
authors.lastname: Dinçkaya
mesh_terms: D000363:Agaricales / Q000166:cytology* / Q000201:enzymology*; D015374:Biosensing Techniques / Q000379:methods*; D002021:Buffers; D002138:Calibration; D004156:Catechol Oxidase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000032:analysis / Q000494:pharmacology; D005504:Food Analysis / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D013447:Sulfites / Q000032:analysis* / Q000494:pharmacology*; D013696:Temperature
chemical_list: D002021:Buffers; D004791:Enzyme Inhibitors; D013447:Sulfites; D004156:Catechol Oxidase