title: A neuronal transmembrane protein LRFN4 induces monocyte/macrophage migration via actin cytoskeleton reorganization.
abstract: Leucine-rich repeat and fibronectin type III domain-containing (LRFN) family proteins are thought to be neuronal-specific proteins that play essential roles in neurite outgrowth and synapse formation. Here, we focused on expression and function of LRFN4, the fourth member of the LRFN family, in non-neural tissues. We found that LRFN4 was expressed in a wide variety of cancer and leukemia cell lines. We also found that expression of LRFN4 in the monocytic cell line THP-1 and in primary monocytes was upregulated following macrophage differentiation. Furthermore, we demonstrated that LRFN4 signaling regulated both the transendothelial migration of THP-1 cells and the elongation of THP-1 cells via actin cytoskeleton reorganization. Our data indicate that LRFN4 signaling plays an important role in the migration of monocytes/macrophages.
authors.affiliation: Department of Medicinal and Life Science, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Noda, Chiba, Japan. s-konaka@rs.noda.tus.ac.jp
authors.forename: Shu
authors.initials: S
authors.lastname: Konakahara
authors.forename: Maki
authors.initials: M
authors.lastname: Saitou
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Hori
authors.forename: Taku
authors.initials: T
authors.lastname: Nakane
authors.forename: Kenko
authors.initials: K
authors.lastname: Murai
authors.forename: Reiko
authors.initials: R
authors.lastname: Itoh
authors.forename: Aya
authors.initials: A
authors.lastname: Shinsaka
authors.forename: Junya
authors.initials: J
authors.lastname: Kohroki
authors.forename: Takao
authors.initials: T
authors.lastname: Kawakami
authors.forename: Masunori
authors.initials: M
authors.lastname: Kajikawa
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Masuho
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology*; D003599:Cytoskeleton / Q000378:metabolism*; D006801:Humans; D000090263:Leucine-Rich Repeat Proteins; D008264:Macrophages / Q000166:cytology / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D009000:Monocytes / Q000166:cytology / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology; D013755:Tetradecanoylphorbol Acetate / Q000378:metabolism; D057705:Transendothelial and Transepithelial Migration / Q000502:physiology
chemical_list: D000199:Actins; C561460:LRFN4 protein, human; D000090263:Leucine-Rich Repeat Proteins; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D011506:Proteins; D013755:Tetradecanoylphorbol Acetate