title: Multi-objective optimization of bioethanol production during cold enzyme starch hydrolysis in very high gravity cassava mash.
abstract: Cold enzymatic hydrolysis conditions for bioethanol production were optimized using multi-objective optimization. Response surface methodology was used to optimize the effects of α-amylase, glucoamylase, liquefaction temperature and liquefaction time on S. cerevisiae biomass, ethanol concentration and starch utilization ratio. The optimum hydrolysis conditions were: 224 IU/g(starch) α-amylase, 694 IU/g(starch) glucoamylase, 77°C and 104 min for biomass; 264 IU/g(starch) α-amylase, 392 IU/g(starch) glucoamylase, 60°C and 85 min for ethanol concentration; 214 IU/g(starch) α-amylase, 398 IU/g(starch) glucoamylase, 79°C and 117 min for starch utilization ratio. The hydrolysis conditions were subsequently evaluated by multi-objectives optimization utilizing the weighted coefficient methods. The Pareto solutions for biomass (3.655-4.380×10(8)cells/ml), ethanol concentration (15.96-18.25 wt.%) and starch utilization ratio (92.50-94.64%) were obtained. The optimized conditions were shown to be feasible and reliable through verification tests. This kind of multi-objective optimization is of potential importance in industrial bioethanol production.
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, Guangdong 510640, PR China.
authors.forename: Bao
authors.initials: B
authors.lastname: Yingling
authors.forename: Chen
authors.initials: C
authors.lastname: Li
authors.forename: Wang
authors.initials: W
authors.lastname: Honglin
authors.forename: Yu
authors.initials: Y
authors.lastname: Xiwen
authors.forename: Yan
authors.initials: Y
authors.lastname: Zongcheng
mesh_terms: D003080:Cold Temperature; D000431:Ethanol / Q000378:metabolism*; D006868:Hydrolysis; D002365:Manihot / Q000201:enzymology / Q000378:metabolism*; D013213:Starch / Q000378:metabolism*; D013499:Surface Properties
chemical_list: D000431:Ethanol; D013213:Starch