title: Overexpression of Nrdp1/FLRF sensitizes cells to oxidative stress.
abstract: Nrdp1 is a RING finger containing ubiquitin E3 ligase that interacts with and modulates activity of multiple proteins, including ErbB3 and Parkin, a causative protein for early onset recessive juvenile parkinsonism (AR-JP). To investigate the functions of Nrdp1, we have generated stable Tet-On inducible HEK293 cells that overexpress Flag-tagged full length Nrdp1, N-terminal Nrdp1 and C-terminal Nrdp1. We demonstrate that overexpression of full-length Nrdp1, not Nrdp1 N-terminus or Nrdp1 C-terminus in cultured HEK293 cells, inhibits cell growth. In addition, we have treated cells with hydroxynonenal (HNE), 6-hydroxydopamine (6-OHDA), and hydrogen peroxide (H(2)O(2)) at different concentrations. We have found that Nrdp1 overexpression sensitizes HEK293 cells to oxidative stressors in a dosage-dependent manner. Our data provide insights into understanding the potential role of Nrdp1 in cell growth, apoptosis and oxidative stress, and in the pathogenesis of Parkinson's disease.
authors.affiliation: JiangSu Key Laboratory of Neuroregeneration, Nantong University, Nantong, JiangSu 226007, PR China.
authors.forename: An
authors.initials: A
authors.lastname: Zhou
authors.forename: Danmin
authors.initials: D
authors.lastname: Pan
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Yang
authors.forename: Jianhua
authors.initials: J
authors.lastname: Zhou
mesh_terms: D017209:Apoptosis / Q000187:drug effects / Q000235:genetics*; D057809:HEK293 Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D018384:Oxidative Stress / Q000235:genetics*; D016627:Oxidopamine / Q000494:pharmacology; D044767:Ubiquitin-Protein Ligases / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D016627:Oxidopamine; D006861:Hydrogen Peroxide; C452580:RNF41 protein, human; D044767:Ubiquitin-Protein Ligases