title: The genetic and physiological analysis of late-flowering phenotype of T-DNA insertion mutants of AtCAL1 and AtCAL2 in Arabidopsis.
abstract: The homozygous T-DNA mutants of AtCAL1 (Rat1) and AtCAL2 (Rat2) were obtained. The double mutant of Rat2/Rat1RNAi was constructed which showed obvious late-flowering phenotype from others. The expression of various flowering-related genes was studied among mutants and wild-type plants by quantitative RT-PCR. The double mutant plants showed the shortest root length compared with T-DNA insertion mutants and wild type plants under red light, blue light, and white light. The double mutants showed hypersensitivity to NaCl and ABA. However, these mutants had no effect on stomatal closure by ABA.
authors.affiliation: College of Biology, State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, China.
authors.forename: Jihong
authors.initials: J
authors.lastname: Zhang
authors.forename: Xinhong
authors.initials: X
authors.lastname: Guo
authors.forename: Xiushan
authors.initials: X
authors.lastname: Li
authors.forename: Feng
authors.initials: F
authors.lastname: Xiang
authors.forename: Bo
authors.initials: B
authors.lastname: Zhou
authors.forename: Dashi
authors.initials: D
authors.lastname: Yu
authors.forename: Dongying
authors.initials: D
authors.lastname: Tang
authors.forename: Xuanming
authors.initials: X
authors.lastname: Liu
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism*; D035264:Flowers / Q000235:genetics* / Q000502:physiology; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D026161:MADS Domain Proteins / Q000235:genetics*; D016254:Mutagenesis, Insertional / Q000235:genetics*; D010641:Phenotype; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D029681:Arabidopsis Proteins; C091536:CAL protein, Arabidopsis; D017931:DNA Primers; D004269:DNA, Bacterial; D026161:MADS Domain Proteins; C036483:T-DNA