title: Exploring polymorphisms of the bovine RARRES2 gene and their associations with growth traits.
abstract: Retinoic acid receptor responder 2 gene (RARRES2) encodes a novel adipokine protein that plays a crucial role in regulating several biological processes, including immune responses, adipocyte differentiation, type 2 diabetes and metabolic syndrome. In this paper, polymorphisms of the bovine RARRES2 gene were detected in 1300 individuals from six breeds by DNA pooling, CRS-PCR-RFLP and DNA sequencing methods. The results showed that NC_007302:g.117035859A>G, 117035706G>A and 117034290A>G were in the coding region, which resulted in three synonymous mutations and only 117033779C>G was in the 3' UTR. Additionally, associations of the four novel SNPs with growth traits were analyzed in Nanyang cattle up to 2 years of age. In P1-PvuII locus, individuals with genotype BC had greater body height and hucklebone width than those with genotype AA, AC and AB at the age of 24 months. In P3-BamHI locus, individuals with genotype AG had higher hucklebone width than those with genotype GG at the age of 24 months. However, no statistically significant differences were observed in P5-SmaI locus. These results indicated that RARRES2 gene might be a potential candidate gene for marker-assisted selection (MAS).
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, 712100 Shaanxi, China. yazhang11@gmail.com
authors.forename: Ya
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jinlong
authors.initials: J
authors.lastname: Zhu
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
authors.forename: Jiajie
authors.initials: J
authors.lastname: Sun
authors.forename: Xinsheng
authors.initials: X
authors.lastname: Lai
authors.forename: Yao
authors.initials: Y
authors.lastname: Xu
authors.forename: Xianyong
authors.initials: X
authors.lastname: Lan
authors.forename: Chuzhao
authors.initials: C
authors.lastname: Lei
authors.forename: Chunlei
authors.initials: C
authors.lastname: Zhang
authors.forename: Donbgying
authors.initials: D
authors.lastname: Yang
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
mesh_terms: D000818:Animals; D001483:Base Sequence; D001837:Body Weights and Measures; D001947:Breeding / Q000379:methods; D002417:Cattle / Q000235:genetics* / Q000254:growth & development*; D017931:DNA Primers / Q000235:genetics; D056726:Genetic Association Studies / Q000662:veterinary; D005838:Genotype; D015233:Models, Statistical; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011110:Polymorphism, Genetic / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D018168:Receptors, Retinoic Acid / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000662:veterinary
chemical_list: D017931:DNA Primers; D018168:Receptors, Retinoic Acid