title: Complexome of Escherichia coli cytosolic proteins under normal native conditions.
abstract: The interactions between proteins are important for the majority of biological functions and the interacting proteins are usually assembled into a complex. Knowing a set of protein complexes of a cell (complexome) is, therefore, essential for a better understanding and global view of cell functions. To visualize and identify the protein complexome of E. coli K-12 under normal native conditions on a proteome-wide scale, we developed an integrated proteomic platform with the combination of 2-D native/SDS-PAGE-based proteomics with co-immunoprecipitation, far-Western blotting, His-tag affinity purification and functional analysis, and used it to investigate the E. coli cytosolic complexome. A total of 24 distinct heteromeric and 8 homomeric protein complexes were identified. These complexes mainly contributed to glycolysis/gluconeogenesis, bioinformation processing, and cellular processes. Of the 24 hetereomeric complexes, 16 were reported for the first time, and 2 known complexes contained novel components that have not been reported previously based on DIP database search. Among them, RpoC-RpsA-Tig-GroL was found to be involved in transcriptional and co-translational folding, and EF-G-TufA-Tsf-RpsA linked a protein synthesis site with protein translational elongation factors. This systematic proteome analysis provides new insights into E. coli molecular systems biology.
authors.affiliation: Lab of proteomics, School of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, 310018, People's Republic of China.
authors.forename: Jian-Yi
authors.initials: JY
authors.lastname: Pan
authors.forename: Hongkai
authors.initials: H
authors.lastname: Wu
authors.forename: Xiang
authors.initials: X
authors.lastname: Liu
authors.forename: Pei-Pei
authors.initials: PP
authors.lastname: Li
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: San-Ying
authors.initials: SY
authors.lastname: Wang
authors.forename: Xuan-Xian
authors.initials: XX
authors.lastname: Peng
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D047468:Immunoprecipitation; D011485:Protein Binding
chemical_list: D029968:Escherichia coli Proteins