title: Gold nanoparticle-hybridized "nano-sponge" polymer coatings to enhance the reliability and sensitivity of biosensors.
abstract: We have created a new functional biosensor coating composed of polyelectrolyte multilayers containing gold nanoparticles. This gold-hybridized polyelectrolyte multilayer film possesses a stable nanoporous structure under physiological conditions. Antibody molecules were successfully conjugated onto the gold nanoparticles within the film. This functional coating successfully extinguished false signals from non-specific binding of proteins and cells and also provided highly enhanced detection sensitivity. Furthermore, the drastic differences in protein and cellular adhesion properties between a chip coated with the nanoporous PEM film and a bare chip demonstrate that morphological control of biological interactions on chip surfaces is possible.
authors.affiliation: Department of Polymer Science and Engineering, Chungnam National University, 220 Gung-Dong, Yuseong-Gu, Daejeon 305-764, Korea.
authors.forename: Hyung-Jun
authors.initials: HJ
authors.lastname: Jeong
authors.forename: Won-Hee
authors.initials: WH
authors.lastname: Pyun
authors.forename: Sung Yun
authors.initials: SY
authors.lastname: Yang