title: Inter- and intra-varietal genetic variability in Malvasia cultivars.
abstract: The DNA molecular analyses together with ampelography, ampelometry, and biochemistry are essential for grapevine identification and investigation of genetic differences among the Vitis vinifera L. cultivars and clones. Ten Malvasia cultivars (i.e., Istrian Malvasia; M. delle Lipari; M. bianca di Candia; M. di Candia Aromatica; M. del Lazio; M. bianca lunga, also known as Malvasia del Chianti; M. nera di Brindisi/Lecce; M. di Casorzo; M. di Schierano, and M. nera di Bolzano) were analyzed using molecular approaches to study the genetic inter-varietal variability. Thirty Istrian Malvasia genotypes (i.e., 8 Italian clones, such as ISV 1, ISV F6, VCR 4, VCR 113, VCR 114, VCR 115, ERSA 120, ERSA 121, and 22 autochthonous grapevine accessions grown in Istrian Peninsula, Croatia) were investigated to evaluate the morphological and genetic intra-varietal variability. DNA analysis allowed discrimination of all Malvasia genotypes at molecular level using AFLP, SAMPL, and M-AFLP markers. Italian clones and autochthonous Croatian accessions of Istrian Malvasia were grouped according to their different geographic origins. These results showed the great genetic variability of Malvasia genotypes suggesting the need for the preservation of autochthonous grapevine biotypes found on different areas to approve the correct choice and selection of the grape multiplication materials.
authors.affiliation: CRA-VIT Consiglio per la Ricerca e la Sperimentazione in Agricoltura, Centro di Ricerca per la Viticoltura, Viale XXVIII Aprile 26, 31015 Conegliano (Treviso), Italy. stefano.meneghetti@entecra.it
authors.forename: Stefano
authors.initials: S
authors.lastname: Meneghetti
authors.forename: Danijela
authors.initials: D
authors.lastname: Poljuha
authors.forename: Enrica
authors.initials: E
authors.lastname: Frare
authors.forename: Angelo
authors.initials: A
authors.lastname: Costacurta
authors.forename: Giacomo
authors.initials: G
authors.lastname: Morreale
authors.forename: Luigi
authors.initials: L
authors.lastname: Bavaresco
authors.forename: Antonio
authors.initials: A
authors.lastname: Calò
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis / Q000379:methods; D003001:Cloning, Molecular; D017523:Croatia; D018744:DNA, Plant / Q000235:genetics / Q000302:isolation & purification; D056426:Genetic Loci; D014644:Genetic Variation; D005838:Genotype; D018895:Microsatellite Repeats; D010641:Phenotype; D017422:Sequence Analysis, DNA; D027843:Vitis / Q000145:classification* / Q000235:genetics*
chemical_list: D018744:DNA, Plant