title: Inhibitory effect of 1α-hydroxyvitamin D3 on N-nitrosobis(2-oxopropyl)amine-induced cholangiocarcinogenesis in Syrian hamsters.
abstract: Sixty-three male 5-week-old Syrian hamsters received the carcinogen N-nitrosobis(2-oxopropyl)amine (BOP) s.c. in 5 weekly injections (the first, 70 mg/kg body, and the remaining, 20mg/kg each). The hamsters that received BOP were given intragastric administration of 0.2 ml of medium chain triglyceride (MCT) with or without 0.04 μg of 1α-hydroxyvitamin D3 [1α(OH)D3] through a feeding tube for 12 weeks. Thus, 3 groups were assigned:Group 1;BOP alone (n＝20), Group 2;BOP＋MCT (n＝18) and Group 3;BOP＋1α(OH)D3 (n＝25). The mean body weight of Group 3 was lower than those of Groups 1 and 2 at the end of the experiment (p＜0.001,Tukey-Kramer HSD test). At the end of week 12, all surviving hamsters were put to sleep. The incidences of liver tumors were 80%, 72% and 32% in Groups 1, 2 and 3, respectively. The incidence of tumors in Group 3 was significantly lower than in Group 1 and Group 2 (p＜0.05, χ2-test). All tumors were cholangiocarcinoma. These results indicated that BOP-induced cholangiocarcinogenesis was suppressed by the supplemental administration of 1α(OH)D3.
authors.affiliation: Department of Physical Therapy, School of Health Care and Social Welfare, KIBI International University, Takahashi, Okayama, Japan. kawaura@kiui.ac.jp
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kawaura
authors.forename: Noritoshi
authors.initials: N
authors.lastname: Tanida
authors.forename: Junichi
authors.initials: J
authors.lastname: Akiyama
authors.forename: Kouji
authors.initials: K
authors.lastname: Nonaka
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Mizutani
authors.forename: Kenji
authors.initials: K
authors.lastname: Sawada
authors.forename: Kimie
authors.initials: K
authors.lastname: Nakagawa
authors.forename: Naoko
authors.initials: N
authors.lastname: Tsugawa
authors.forename: Keisuke
authors.initials: K
authors.lastname: Izumi
authors.forename: Kunio
authors.initials: K
authors.lastname: Ii
authors.forename: Toshio
authors.initials: T
authors.lastname: Okano
authors.forename: Eiji
authors.initials: E
authors.lastname: Takeda
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001650:Bile Duct Neoplasms / Q000139:chemically induced* / Q000517:prevention & control; D001653:Bile Ducts, Intrahepatic / Q000473:pathology*; D002273:Carcinogens / Q000633:toxicity; D018281:Cholangiocarcinoma / Q000139:chemically induced* / Q000517:prevention & control; D002762:Cholecalciferol / Q000494:pharmacology*; D006224:Cricetinae; D008297:Male; D008647:Mesocricetus; D009602:Nitrosamines / Q000633:toxicity*
chemical_list: D000970:Antineoplastic Agents; D002273:Carcinogens; D009602:Nitrosamines; D002762:Cholecalciferol; C013622:nitrosobis(2-oxopropyl)amine