title: [Spectral study on the interaction of [Cu(DPPZ)(L-Ser)]+ complex with DNA and its analytical application].
abstract: The characteristics of resonance light scattering (RLS), UV-visible absorption spectra and fluorescence spectra of [Cu(DPPZ)(L-Ser)]+ with DNA were studied and a RLS method for the determination of DNA was established. [Cu(DPPZ) (L-Ser)]+ could assemble on the surface of DNA through intercalation, and enhanced the RLS of DNA in the tris buffer of pH 7. 2. The maximum resonance light scattering peak appeared at 400 nm. Under the optimum conditions, the enhanced intensity of RLS was proportional to the concentration of DNA over the range of 0.42 - 4.2 ng x mL(-1), with a detection limit (3sigma/k) of 0.29 ng x mL(-1). The method was used for the determination of DNA samples with the recoveries between 97.8% and 106%.
authors.affiliation: School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Cheng-Ke
authors.initials: CK
authors.lastname: Wu
authors.forename: Qiu-Peng
authors.initials: QP
authors.lastname: Dong
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.forename: Cen
authors.initials: C
authors.lastname: Xiong
authors.forename: Xiu-Li
authors.initials: XL
authors.lastname: Wu
authors.forename: Lian-Sheng
authors.initials: LS
authors.lastname: Ling
mesh_terms: D004247:DNA / Q000737:chemistry*; D008027:Light; D057230:Limit of Detection; D008055:Lipids / Q000737:chemistry; D009942:Organometallic Compounds / Q000737:chemistry*; D012542:Scattering, Radiation; D012694:Serine / Q000031:analogs & derivatives / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D014732:Vibration
chemical_list: D008055:Lipids; D009942:Organometallic Compounds; C069989:serine containing aminolipid; D012694:Serine; D004247:DNA