title: C-T variant in a miRNA target site of BCL2 is associated with increased risk of human papilloma virus related cervical cancer--an in silico approach.
abstract: MicroRNAs control gene expression at the posttranscriptional level by base-pairing to the 3'-UTR of their target mRNAs, thus leading to mRNA degradation of protein fabrication. We hypothesize, SNPs within miRNAs and their targets could be of significance to an individual's risk of developing cancer. We analyzed in silico SNP information on cervical cancer associated aberrant alleles and further investigated this in a case-control study by examining eleven SNPs from different genes. It was observed that a C to T polymorphism in putative miRNA target site of BCL2 was significantly conspicuous for the aberrant SNP allele in cancer tissues as compared to controls. This study provides evidence that SNPs in miRNA-binding sites may play an important role in increasing risk of cancer. The results also paves way for future studies to validate these results in other well-characterized populations as well as to explore the biological significance of these particular SNPs.
authors.affiliation: Integrated Cancer Research Program, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram-695014, Kerala, India. reshmi@rgcb.res.in
authors.forename: G
authors.initials: G
authors.lastname: Reshmi
authors.forename: Ramachandran
authors.initials: R
authors.lastname: Surya
authors.forename: V T
authors.initials: VT
authors.lastname: Jissa
authors.forename: P S Saneesh
authors.initials: PS
authors.lastname: Babu
authors.forename: N R
authors.initials: NR
authors.lastname: Preethi
authors.forename: W S
authors.initials: WS
authors.lastname: Santhi
authors.forename: P G
authors.initials: PG
authors.lastname: Jayaprakash
authors.forename: M Radhakrishna
authors.initials: MR
authors.lastname: Pillai
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000483:Alleles; D000595:Amino Acid Sequence; D001665:Binding Sites; D016022:Case-Control Studies; D019295:Computational Biology; D005260:Female; D055106:Genome-Wide Association Study / Q000379:methods; D005838:Genotype; D006367:HeLa Cells; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D027383:Papillomaviridae / Q000472:pathogenicity*; D020641:Polymorphism, Single Nucleotide; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000821:virology*; D044465:White People / Q000235:genetics
chemical_list: D020413:3' Untranslated Regions; D035683:MicroRNAs; D019253:Proto-Oncogene Proteins c-bcl-2