title: Expression of coagulation-related protein genes during LPS-induced preterm delivery in the pregnant mouse.
abstract: Preterm delivery (PTD) has been associated with inflammation along with activation of the coagulation pathway. These studies sought to characterize the expression of several coagulation pathway genes including plasminogen activator inhibitor 1 (PAI-1), tissue factor (TF), protease-activated receptor 1 (Par1), protease-activated receptor 2 (Par2), fibrinogen-like protein 2 (Fgl2), and thrombomodulin (TM) during lipopolysaccharide (LPS)-induced PTD in day 15 pregnant CD-1 mice. Western blot studies confirmed protein expression for PAI-1, Par1, Par2, Fgl2, and TM in the mouse uterus. Quantitative reverse transcriptase polymerase chain reaction (RT-PCR) confirmed increased PAI-1 messenger RNA (mRNA) in the uteri, lung, kidney, and liver tissues at 2 to 6 hours after LPS injection. In contrast, TF expression significantly decreased by 12 hours in uterine tissue; whereas, its expression was unchanged in the other maternal tissues. The uterine mRNA for Par1, Par2, Fgl2, and TM remained stable. In summary, these studies have confirmed expression of coagulation pathway genes within the pregnant uterus; some of which are modulated during LPS-induced PTD.
authors.affiliation: Department of Obstetrics, Gynecology and Reproductive Sciences, College of Medicine, University of Vermont, Burlington, VT 05405, USA. mark.phillippe@uvm.edu
authors.forename: Mark
authors.initials: M
authors.lastname: Phillippe
authors.forename: Allaire K
authors.initials: AK
authors.lastname: Diamond
authors.forename: Leigh M
authors.initials: LM
authors.lastname: Sweet
authors.forename: Karen H
authors.initials: KH
authors.lastname: Oppenheimer
authors.forename: Diana F
authors.initials: DF
authors.lastname: Bradley
mesh_terms: D000818:Animals; D001777:Blood Coagulation / Q000235:genetics*; D005260:Female; D005340:Fibrinogen / Q000235:genetics; D015870:Gene Expression; D008070:Lipopolysaccharides; D051379:Mice; D017395:Plasminogen Activator Inhibitor 1 / Q000235:genetics; D011247:Pregnancy; D047928:Premature Birth / Q000139:chemically induced / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D044463:Receptor, PAR-1 / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018180:Thrombomodulin / Q000235:genetics; D013925:Thromboplastin / Q000235:genetics; D014599:Uterus / Q000737:chemistry
chemical_list: C490637:Fgl2 protein, mouse; D008070:Lipopolysaccharides; D017395:Plasminogen Activator Inhibitor 1; D012333:RNA, Messenger; D044463:Receptor, PAR-1; D018180:Thrombomodulin; D005340:Fibrinogen; D013925:Thromboplastin