title: Improvement of trivalent leptospira vaccine by removal of anaphylactic agents.
abstract: The anaphylactic reactions in cattle following leptospira vaccination mostly booster dose in different parts of Iran have been reported. The serum proteins as allergic substances are components of liquid phase of the vaccine. Therefore, the vaccine was modified by washing the whole cultures by centrifugations. The modified vaccine was safe in laboratory animals and cattle as well as under field conditions. Microagglutination test revealed a similar pattern of antibody response to the three Leptospira interrogans serovars (Canicola, Grippotyphosa, and Sejro hardjo) in all vaccinated cattle groups while was higher than the response of control animals. The results of the present investigation revealed that we can minimize postvaccination shock in vaccinated cattle populations with removing the shock proteins.
authors.affiliation: Razi Vaccine and Serum Research Institute, Tehran, Iran. moazenjula@yahoo.com
authors.forename: Gholamreza
authors.initials: G
authors.lastname: Moazenijula
authors.forename: A R
authors.initials: AR
authors.lastname: Jabbari
authors.forename: M Moradi
authors.initials: MM
authors.lastname: Geravand
authors.forename: R
authors.initials: R
authors.lastname: Banihashemi
authors.forename: A
authors.initials: A
authors.lastname: Hajizadeh
mesh_terms: D000372:Agglutination Tests / Q000662:veterinary; D000707:Anaphylaxis / Q000139:chemically induced / Q000517:prevention & control / Q000662:veterinary*; D000818:Animals; D000830:Animals, Laboratory / Q000276:immunology; D000907:Antibodies, Bacterial / Q000032:analysis; D000917:Antibody Formation; D001428:Bacterial Vaccines / Q000276:immunology*; D001798:Blood Proteins / Q000276:immunology*; D002417:Cattle; D002418:Cattle Diseases / Q000188:drug therapy / Q000276:immunology* / Q000517:prevention & control; D002498:Centrifugation / Q000662:veterinary; D007492:Iran; D007921:Leptospira interrogans / Q000276:immunology; D007922:Leptospirosis / Q000188:drug therapy / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D008297:Male; D014611:Vaccination / Q000662:veterinary
chemical_list: D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D001798:Blood Proteins