title: Fructose 1-6 bisphosphate versus University of Wisconsin solution for rat liver preservation: does FBP prevent early mitochondrial injury?
abstract: BACKGROUND
  Fructose 1,6-biphosphate (FBP) has been shown to exert therapeutic effects in models of ischemia-reperfusion in organs other than the liver. This study compared FBP and University of Wisconsin (UW) solution during cold storage and reperfusion, among mitochondria of adult male Wistar rat livers.
  
  
  METHODS
  Adult male Wistar rats were assigned to two groups according to the preservation solution used; UW or FBP Aspartate transaminase (AST), alanine transferase (ALT); and lactic dehydrogenase (LDH) were measured in samples of the storage solution obtained at 2, 4 and 6 hours of preservation. After 6 hours of cold storage, we reperfused the liver, taking blood samples to measure AST, ALT, LDH, and throbarbituric acid reactive substances (TBARS). Hepatic fragments were processed for histologic analysis; for determinations of TBARS, catalase, and nitric oxide as well as for mitochondrial evaluation by infrared spectroscopy.
  
  
  RESULTS
  During cold preservation, levels of AST and LDH in the storage solution were lower among the FBP group, but after reperfusion, serum levels of AST, ALT, and LDH were higher in this group, as was catalase activity. TBARS and nitric oxide were comparable between the groups. In the UW group there was a higher amide I/amide II ratio than in the FBP group, suggesting an abnormal protein structure of the mitochondrial membrane. No signs of preservation injury were observed in any liver biopsy, but sinusoidal congestion was present in livers preserved with FBP.
  
  
  CONCLUSIONS
  FBP showed a protective effect for preservation during cold storage seeming to protect the mitochondrial membrane although it did not prevent reperfusion injury.
authors.affiliation: Post-Graduate in Gastroenterology, Universidade Federal do Rio Grande do Sul, RS, Brazil. rschfraga@terra.com.br
authors.forename: R S
authors.initials: RS
authors.lastname: de Fraga
authors.forename: P E T
authors.initials: PE
authors.lastname: Heinen
authors.forename: C R P
authors.initials: CR
authors.lastname: Kruel
authors.forename: S D
authors.initials: SD
authors.lastname: Molin
authors.forename: S M
authors.initials: SM
authors.lastname: Mota
authors.forename: C T S
authors.initials: CT
authors.lastname: Cerski
authors.forename: G
authors.initials: G
authors.lastname: Gasperin
authors.forename: A A
authors.initials: AA
authors.lastname: Souto
authors.forename: J R
authors.initials: JR
authors.lastname: de Oliveira
authors.forename: M R
authors.initials: MR
authors.lastname: Alvares-da-Silva
mesh_terms: D000818:Animals; D005635:Fructosediphosphates / Q000008:administration & dosage* / Q000494:pharmacology; D008099:Liver; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000473:pathology; D011309:Preservation, Biological; D051381:Rats; D017208:Rats, Wistar; D012996:Solutions
chemical_list: D005635:Fructosediphosphates; D012996:Solutions; C029063:fructose-1,6-diphosphate