title: Ultraviolet-B irradiation induces differential regulations of hyaluronidase expression and activity in normal human keratinocytes.
abstract: Skin aging is a complex process determined by genetic factors (intrinsic aging) and environmental factors (extrinsic aging). One of the most influential environmental factor is UV-B irradiation. Hyaluronic acid (HA) is an abundant component of skin extracellular matrix where it plays many roles such as hydration and architectural support. Downregulation of HA during photoaging was reported previously. Changes in expression and function of its degrading enzymes, the hyaluronidases (Hyals) might be involved in this decrease. In the present study, normal human keratinocytes were submitted to increasing doses of UV-B. The mRNA expression of HYAL1, HYAL2 and HYAL3 and the hyaluronidase enzymatic activity were quantified using real-time PCR and a microtiter-based assay, respectively. After UV-B irradiation, HYAL1 mRNA expression was upregulated whereas HYAL2 and HYAL3 mRNAs were downregulated and hyaluronidase enzymatic activity was increased in both cell layer and culture medium. In parallel, immunohistochemical studies performed on UV-B irradiated reconstructed epidermis confirmed that Hyal-1, Hyal-2 and Hyal-3 protein expression were differently regulated by UV-B. Taken together, our results demonstrate that UV-B irradiation induces differential regulations of hyaluronidase expression and enzymatic activity in human keratinocytes. These differential modulations of hyaluronidase expression and activity by UV-B could contribute to cutaneous photoaging.
authors.affiliation: Laboratoire de Biochimie Médicale et Biologie Moléculaire, CNRS UMR 6237, Université de Reims Champagne-Ardenne, Reims, France.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Kurdykowski
authors.forename: Solène
authors.initials: S
authors.lastname: Mine
authors.forename: Vincent
authors.initials: V
authors.lastname: Bardey
authors.forename: Louis
authors.initials: L
authors.lastname: Danoux
authors.forename: Christine
authors.initials: C
authors.lastname: Jeanmaire
authors.forename: Gilles
authors.initials: G
authors.lastname: Pauly
authors.forename: Eva
authors.initials: E
authors.lastname: Brabencova
authors.forename: Yanusz
authors.initials: Y
authors.lastname: Wegrowski
authors.forename: François Xavier
authors.initials: FX
authors.lastname: Maquart
mesh_terms: D000951:Antigens, Neoplasm / Q000235:genetics / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation; D004817:Epidermis / Q000378:metabolism / Q000528:radiation effects; D005109:Extracellular Matrix / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects*; D058851:GPI-Linked Proteins / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000528:radiation effects*; D051548:Histone Acetyltransferases / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D006820:Hyaluronic Acid / Q000378:metabolism; D006821:Hyaluronoglucosaminidase / Q000235:genetics / Q000378:metabolism*; D007150:Immunohistochemistry; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects*; D061251:Primary Cell Culture; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D060888:Real-Time Polymerase Chain Reaction; D015595:Skin Aging / Q000528:radiation effects*; D015854:Up-Regulation
chemical_list: D000951:Antigens, Neoplasm; D015815:Cell Adhesion Molecules; D058851:GPI-Linked Proteins; D012333:RNA, Messenger; D006820:Hyaluronic Acid; D051548:Histone Acetyltransferases; C115866:OGA protein, human; C473096:Hyal2 protein, human; D006821:Hyaluronoglucosaminidase; C066852:hyaluronidase PH-20