title: Anti-spermatogenic activity of ethanol extract of Dalbergia sissoo Roxb. stem bark.
abstract: The study was undertaken to evaluate the anti-spermatogenic efficacy of ethanol extract of stem bark of Dalbergia sissoo Roxb. For the in vitro study, semen samples were obtained from 15 healthy fertile men aged 25-35 years. Sperm motility was examined by the Sander-Cramer method. A dose-dependent and time-dependent effect of ethanol extract on sperm motility and sperm viability were observed. Various concentrations affected the motility of sperm. Ethanol extract at a concentration of 20 mg/mL caused complete immobilization within 3 minutes. Sperm viability and hypo-osmotic swelling was significantly reduced at this concentration. The in vivo studies were carried out on Swiss male albino mice. Ethanol extract at a dose of 200 mg/kg body weight resulted in a significant decrease (p<0.001) in weight of the testis and epididymis. A significant decrease (p<0.01) in sperm motility and sperm count in the epididymis were observed. Histological changes in the epididymis and testis were also investigated.
authors.affiliation: Division of Pharmacognosy, Department of Pharmaceutical Sciences, Guru Jambheshwar University of Science and Technology, Haryana, India. neeruvasudeva@gmail.com
authors.forename: Neeru
authors.initials: N
authors.lastname: Vasudeva
authors.forename: Manisha
authors.initials: M
authors.lastname: Vats
mesh_terms: D000328:Adult; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D035782:Dalbergia; D004305:Dose-Response Relationship, Drug; D004822:Epididymis / Q000187:drug effects; D006801:Humans; D008297:Male; D051379:Mice; D009929:Organ Size / Q000187:drug effects; D009997:Osmotic Pressure / Q000187:drug effects; D024301:Plant Bark; D010936:Plant Extracts / Q000494:pharmacology*; D018547:Plant Stems; D013076:Sperm Count; D013081:Sperm Motility / Q000187:drug effects*; D013091:Spermatogenesis / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects* / Q000502:physiology; D013737:Testis / Q000187:drug effects
chemical_list: D010936:Plant Extracts