title: Sedimentation velocity analysis of amyloid fibrils.
abstract: Analytical ultracentrifugation is a classical technique used to study the solution behavior of proteins. Experimentally determined sedimentation coefficients provide information regarding the size, shape, and interactions of biological macromolecules. Sedimentation velocity methods have been used to characterize the different aggregation states of amyloid oligomers and fibrils. This chapter first describes the theoretical background for sedimentation velocity analysis. It then provides experimental protocols for sedimentation velocity experiments using the analytical ultracentrifuge. Finally, this chapter describes the procedure used to analyze sedimentation velocity data to obtain the size distribution of amyloid fibrils and their oligomeric precursors.
authors.affiliation: Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, VIC, Australia.
authors.forename: Chi Le Lan
authors.initials: Cle L
authors.lastname: Pham
authors.forename: Yee-Foong
authors.initials: YF
authors.lastname: Mok
authors.forename: Geoffrey J
authors.initials: GJ
authors.lastname: Howlett
mesh_terms: D000682:Amyloid / Q000737:chemistry* / Q000378:metabolism; D055503:Protein Multimerization; D017433:Protein Structure, Secondary; D014461:Ultracentrifugation / Q000379:methods*
chemical_list: D000682:Amyloid