title: The complete genome sequence of Clostridium botulinum F str. 230613, insertion sites, and recombination of BoNT gene clusters.
abstract: The genomic DNA of Clostridium botulinum F str. 230613 includes a chromosome (3 993 083 bp, 3502 coding sequences (CDs)) and a plasmid (17 531 bp, 25 CDs). The arrangement of the botulinum neurotoxin serotype F (BoNT/F) gene cluster, a 15-kb (or longer) fragment including the bont gene and other relevant genes, and its different insertion sites in C. botulinum A2 and C. botulinum F were formulated. Mobile elements and virulence factors were analysed. We also found a cell adhesion and pectin lyase domain-containing protein, which may function in attaching to the host and as a pectin lyase. The nine BoNT gene clusters of group I C. botulinum strains were located at three sites in the chromosome of C. botulinum F str. 230613. This study showed the inserting inclination of BoNT/A1 tend to have gene clusters inserted at site 3, BoNT/F at site 2, and BoNT/A2 at site 1. Additionally, we found the recombination event between the BoNT gene clusters of sites 2 and 3, a mechanism that contributed to the diversity of the BoNT gene cluster arrangement.
authors.affiliation: State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Fengtai District, P.R. China.
authors.forename: Ren-Mao
authors.initials: RM
authors.lastname: Tian
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Hou
authors.forename: Qin
authors.initials: Q
authors.lastname: Wang
authors.forename: Kun
authors.initials: K
authors.lastname: Cai
authors.forename: Yue-Nan
authors.initials: YN
authors.lastname: Liu
authors.forename: Xiang
authors.initials: X
authors.lastname: Gao
authors.forename: Hao
authors.initials: H
authors.lastname: Liu
authors.forename: Le
authors.initials: L
authors.lastname: Xiao
authors.forename: Wei
authors.initials: W
authors.lastname: Tu
authors.forename: Jing
authors.initials: J
authors.lastname: Shi
authors.forename: Wu-Chun
authors.initials: WC
authors.lastname: Cao
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
mesh_terms: D001905:Botulinum Toxins / Q000235:genetics*; D002876:Chromosomes, Bacterial / Q000235:genetics; D047971:Clostridium botulinum type F / Q000235:genetics* / Q000472:pathogenicity; D055028:Comparative Genomic Hybridization; D023061:Gene Order; D016680:Genome, Bacterial / Q000235:genetics*; D020071:Interspersed Repetitive Sequences / Q000235:genetics; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D016254:Mutagenesis, Insertional / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D011995:Recombination, Genetic / Q000235:genetics*; D037521:Virulence Factors / Q000235:genetics
chemical_list: D037521:Virulence Factors; D001905:Botulinum Toxins