title: Molecular staging of marine medaka: a model organism for marine ecotoxicity study.
abstract: Oryzias melastigma, also called O. dancena, is becoming a very useful model for estuarine and marine ecotoxicity studies. With O. melastigma being adopted by ILSI Health and Environmental Science Institute (HESI) for embryo toxicity testing, improved knowledge of biomarker based embryonic development becomes especially important for mechanism-based toxicity evaluations. Using whole mount in situ hybridization and immunostaining techniques together with widely used molecular markers, this study describes the molecular development of marine medaka embryos, focusing on the brain, eye, heart, pectoral fin, pancreas, liver, muscle and neuron system. These organs are targets of environmental pollutants that disrupt normal embryonic development in medaka and other fish.
authors.affiliation: State Key Laboratory in Marine Pollution, Department of Biology and Chemistry, City University of Hong Kong, Hong Kong Special Administrative Region.
authors.forename: Xueping
authors.initials: X
authors.lastname: Chen
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Jinping
authors.initials: J
authors.lastname: Cheng
authors.forename: Leo Lai
authors.initials: LL
authors.lastname: Chan
authors.forename: Da-Zhi
authors.initials: DZ
authors.lastname: Wang
authors.forename: Ke-Jian
authors.initials: KJ
authors.lastname: Wang
authors.forename: Michael E
authors.initials: ME
authors.lastname: Baker
authors.forename: Gary
authors.initials: G
authors.lastname: Hardiman
authors.forename: Daniel
authors.initials: D
authors.lastname: Schlenk
authors.forename: Shuk Han
authors.initials: SH
authors.lastname: Cheng
mesh_terms: D000818:Animals; D001483:Base Sequence; D015415:Biomarkers / Q000378:metabolism; D001921:Brain / Q000002:abnormalities / Q000187:drug effects / Q000196:embryology; D054750:Ecotoxicology; D004625:Embryo, Nonmammalian / Q000187:drug effects / Q000378:metabolism; D005124:Eye Abnormalities / Q000139:chemically induced / Q000196:embryology; D006330:Heart Defects, Congenital / Q000139:chemically induced / Q000196:embryology; D008099:Liver / Q000002:abnormalities / Q000196:embryology; D023421:Models, Animal; D008969:Molecular Sequence Data; D009132:Muscles / Q000002:abnormalities / Q000196:embryology; D017570:MyoD Protein / Q000235:genetics; D009990:Oryzias / Q000196:embryology / Q000235:genetics / Q000378:metabolism*; D010179:Pancreas / Q000002:abnormalities / Q000196:embryology; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D017570:MyoD Protein; D014874:Water Pollutants, Chemical