title: PW1 gene/paternally expressed gene 3 (PW1/Peg3) identifies multiple adult stem and progenitor cell populations.
abstract: A variety of markers are invaluable for identifying and purifying stem/progenitor cells. Here we report the generation of a murine reporter line driven by Pw1 that reveals cycling and quiescent progenitor/stem cells in all adult tissues thus far examined, including the intestine, blood, testis, central nervous system, bone, skeletal muscle, and skin. Neurospheres generated from the adult PW1-reporter mouse show near 100% reporter-gene expression following a single passage. Furthermore, epidermal stem cells can be purified solely on the basis of reporter-gene expression. These cells are clonogenic, repopulate the epidermal stem-cell niches, and give rise to new hair follicles. Finally, we demonstrate that only PW1 reporter-expressing epidermal cells give rise to follicles that are capable of self-renewal following injury. Our data demonstrate that PW1 serves as an invaluable marker for competent self-renewing stem cells in a wide array of adult tissues, and the PW1-reporter mouse serves as a tool for rapid stem cell isolation and characterization.
authors.affiliation: Myology Group (Stem Cell and Muscle Biology), Unité Mixte de Recherche-S787 Institut National de la Santé et de la Recherche Médicale, University of Pierre and Marie Curie Paris VI, Paris 75634, France.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Besson
authors.forename: Piera
authors.initials: P
authors.lastname: Smeriglio
authors.forename: Amélie
authors.initials: A
authors.lastname: Wegener
authors.forename: Frédéric
authors.initials: F
authors.lastname: Relaix
authors.forename: Brahim
authors.initials: B
authors.lastname: Nait Oumesmar
authors.forename: David A
authors.initials: DA
authors.lastname: Sassoon
authors.forename: Giovanna
authors.initials: G
authors.lastname: Marazzi
mesh_terms: D053687:Adult Stem Cells / Q000166:cytology* / Q000378:metabolism*; D000818:Animals; D019070:Cell Lineage / Q000235:genetics; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism; D000078404:Epidermal Cells; D004817:Epidermis / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental; D017930:Genes, Reporter; D005819:Genetic Markers; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism; D017403:In Situ Hybridization; D051741:Kruppel-Like Transcription Factors / Q000235:genetics*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D011247:Pregnancy
chemical_list: D005819:Genetic Markers; D051741:Kruppel-Like Transcription Factors; C097901:Peg3 protein, mouse