title: [The rapid analysis of polychlorinated quaterphenyls in blood using different diameter capillary column with the high-resolution gas chromatograph high-resolution mass spectrometer].
abstract: The polychlorinated quaterphenyl (PCQ) concentrations in blood are important discriminative parameters in yusho patient. In this study, a rapid analytical method for PCQ using different diameter capillary column (rapid-Rtx65TG) with high-resolution gas chromatograph high-resolution mass spectrometer (HRGC/HRMS) instead of the gas chromatograph electron capture detector (ECD/GC) was developed. Using different diameter capillary columns, the analysis time of the HRGC/HRMS was drastically shortened, and the detection sensitivity was improved. In the rapid-Rtx65TG column, a small-bore capillary column (length 1m, I.D. 0.1mm) was connected with the inlet side of the GC, and behind that column, a large-bore capillary column (length 15mm, I.D. 0.53mm) for octadecachloroquaterphenyl (ODCQ) analysis was connected. In the HRGC/HRMS measurement of ODCQ by the rapid-Rtx65TG column, the minimum limit of detection for the apparatus was 0.4 pg, and the minimum limit of determination for the blood was 0.008 ppb. On ECD/GC in the conventional method and HRGC/HRMS in this study, the PCQ concentration in blood including yusho patients and yusho suspected persons was almost equivalent.
authors.affiliation: Fukuoka Institute of Health and Environmental Sciences, Fukuoka, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Yasutake
authors.forename: Yuki
authors.initials: Y
authors.lastname: Ashizuka
authors.forename: Tsuguhide
authors.initials: T
authors.lastname: Hori
authors.forename: Youichi
authors.initials: Y
authors.lastname: Kurokawa
authors.forename: Jumboku
authors.initials: J
authors.lastname: Kajiwara
authors.forename: Teruaki
authors.initials: T
authors.lastname: Hirata
authors.forename: Yasuhisa
authors.initials: Y
authors.lastname: Ishiguro
authors.forename: Takao
authors.initials: T
authors.lastname: Iida
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Uchi
authors.forename: Masutaka
authors.initials: M
authors.lastname: Furue
mesh_terms: D002722:Chlorobenzenes / Q000097:blood*; D005506:Food Contamination; D008401:Gas Chromatography-Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D012275:Oryza / Q000506:poisoning; D010938:Plant Oils / Q000506:poisoning; D011078:Polychlorinated Biphenyls / Q000506:poisoning
chemical_list: D002722:Chlorobenzenes; D010938:Plant Oils; C022053:polychlorinated quaterphenyls; D011078:Polychlorinated Biphenyls