title: Cutaneous vascular and core temperature responses to sustained cold exposure in hypoxia.
abstract: We tested the effect of hypoxia on cutaneous vascular regulation and defense of core temperature during cold exposure. Twelve subjects had two microdialysis fibres placed in the ventral forearm and were immersed to the sternum in a bathtub on parallel study days (normoxia and poikilocapnic hypoxia with an arterial O(2) saturation of 80%). One fibre served as the control (1 mM propranolol) and the other received 5 mM yohimbine (plus 1 mM propranolol) to block adrenergic receptors. Skin blood flow was assessed at each site (laser Doppler flowmetry), divided by mean arterial pressure to calculate cutaneous vascular conductance (CVC), and scaled to baseline. Cold exposure was first induced by a progressive reduction in water temperature from 36 to 23°C over 30 min to assess cutaneous vascular regulation, then by clamping the water temperature at 10°C for 45 min to test defense of core temperature. During normoxia, cold stress reduced CVC in control (-44 ± 4%) and yohimbine sites (-13 ± 7%; both P < 0.05 versus precooling). Hypoxia caused vasodilatation prior to cooling but resulted in greater reductions in CVC in control (-67 ± 7%) and yohimbine sites (-35 ± 11%) during cooling (both P < 0.05 versus precooling; both P < 0.05 versus normoxia). Core cooling rate during the second phase of cold exposure was unaffected by hypoxia (-1.81 ± 0.23°C h(-1) in normoxia versus -1.97 ± 0.33°C h(-1) in hypoxia; P > 0.05). We conclude that hypoxia increases cutaneous (non-noradrenergic) vasoconstriction during prolonged cold exposure, while core cooling rate is not consistently affected.
authors.affiliation: Department of Human Physiology, University of Oregon, Eugene, OR 97403, USA.
authors.forename: Grant H
authors.initials: GH
authors.lastname: Simmons
authors.forename: Zachary
authors.initials: Z
authors.lastname: Barrett-O'Keefe
authors.forename: Christopher T
authors.initials: CT
authors.lastname: Minson
authors.forename: John R
authors.initials: JR
authors.lastname: Halliwill
mesh_terms: D001831:Body Temperature / Q000502:physiology*; D003080:Cold Temperature; D005260:Female; D005712:Galvanic Skin Response; D006801:Humans; D000860:Hypoxia / Q000503:physiopathology*; D017078:Laser-Doppler Flowmetry; D008297:Male; D017551:Microdialysis; D010101:Oxygen Consumption; D011433:Propranolol / Q000008:administration & dosage / Q000494:pharmacology; D012039:Regional Blood Flow / Q000187:drug effects; D012867:Skin / Q000098:blood supply*; D012879:Skin Physiological Phenomena; D012881:Skin Temperature / Q000502:physiology*; D014661:Vasoconstriction / Q000187:drug effects / Q000502:physiology*; D014664:Vasodilation / Q000187:drug effects; D015016:Yohimbine / Q000008:administration & dosage / Q000494:pharmacology; D055815:Young Adult
chemical_list: D015016:Yohimbine; D011433:Propranolol