title: In vivo imaging of near-membrane calcium ions with two-photon probes.
abstract: We report a two-photon (TP) probe (ACaLN) for near-membrane Ca(2+) that shows a 13-fold TP excited fluorescence (TPEF) enhancement in response to Ca(2+), dissociation constants (K(d)(TP)) of (1.9±0.2) μM, pH-insensitivity at the biologically relevant pH, and can detect near-membrane Ca(2+) in live cells for more than 1500 s and in living tissues at 120 μm depth without interference from other metal ions. Comparison with existing probes provides a useful strategy for the design of efficient TP probes for the near-membrane metal ions.
authors.affiliation: Department of Chemistry, Korea University, 1-Anamdong, Seoul, 136-701, Korea.
authors.forename: Chang Su
authors.initials: CS
authors.lastname: Lim
authors.forename: Min Young
authors.initials: MY
authors.lastname: Kang
authors.forename: Ji Hee
authors.initials: JH
authors.lastname: Han
authors.forename: Isravel Antony
authors.initials: IA
authors.lastname: Danish
authors.forename: Bong Rae
authors.initials: BR
authors.lastname: Cho
mesh_terms: D000818:Animals; D002118:Calcium / Q000032:analysis*; D002413:Cations, Divalent / Q000032:analysis*; D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry* / Q000648:ultrastructure; D005456:Fluorescent Dyes / Q000737:chemistry*; D006624:Hippocampus / Q000166:cytology*; D051379:Mice; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods
chemical_list: D002413:Cations, Divalent; D005456:Fluorescent Dyes; D002118:Calcium