title: Internal standard-based analysis of microarray data2--analysis of functional associations between HVE-genes.
abstract: In this work we apply the Internal Standard-based analytical approach that we described in an earlier communication and here we demonstrate experimental results on functional associations among the hypervariably-expressed genes (HVE-genes). Our working assumption was that those genetic components, which initiate the disease, involve HVE-genes for which the level of expression is undistinguishable among healthy individuals and individuals with pathology. We show that analysis of the functional associations of the HVE-genes is indeed suitable to revealing disease-specific differences. We show also that another possible exploit of HVE-genes for characterization of pathological alterations is by using multivariate classification methods. This in turn offers important clues on naturally occurring dynamic processes in the organism and is further used for dynamic discrimination of groups of compared samples. We conclude that our approach can uncover principally new collective differences that cannot be discerned by individual gene analysis.
authors.affiliation: Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA. igor-dozmorov@omrf.org
authors.forename: Igor M
authors.initials: IM
authors.lastname: Dozmorov
authors.forename: James
authors.initials: J
authors.lastname: Jarvis
authors.forename: Ricardo
authors.initials: R
authors.lastname: Saban
authors.forename: Doris M
authors.initials: DM
authors.lastname: Benbrook
authors.forename: Edward
authors.initials: E
authors.lastname: Wakeland
authors.forename: Ivona
authors.initials: I
authors.lastname: Aksentijevich
authors.forename: John
authors.initials: J
authors.lastname: Ryan
authors.forename: Nicholas
authors.initials: N
authors.lastname: Chiorazzi
authors.forename: Joel M
authors.initials: JM
authors.lastname: Guthridge
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Drewe
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: Tighe
authors.forename: Michael
authors.initials: M
authors.lastname: Centola
authors.forename: Ivan
authors.initials: I
authors.lastname: Lefkovits
mesh_terms: D016000:Cluster Analysis; D003627:Data Interpretation, Statistical; D004194:Disease / Q000235:genetics; D015870:Gene Expression; D020869:Gene Expression Profiling / Q000379:methods* / Q000592:standards; D053263:Gene Regulatory Networks; D014644:Genetic Variation; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods* / Q000592:standards; D012015:Reference Standards; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D014409:Tumor Necrosis Factor-alpha