title: Increased expression of sodium channel subunit Nav1.1 in the injured dorsal root ganglion after peripheral nerve injury.
abstract: The mechanisms underlying neuropathic pain induction are very complex but might involve abnormal spontaneous activity in the sensory dorsal root ganglion (DRG). Voltage-gated sodium channels in the DRG are essential for the genesis of abnormal spontaneous neuronal activity. In this study, we examined the changes in expression of the voltage-gated sodium channel Nav1.1 in the DRG after peripheral nerve injury. Western blot analysis showed that the level of Nav1.1 protein in the ipsilateral L5 DRG was significantly increased on Days 3 and 7 after fifth lumbar spinal nerve ligation. Immunohistochemical study further confirmed a marked increase in the percentage of Nav1.1-positive cells in the ipsilateral DRG on Day 3 after fifth lumbar spinal nerve ligation. Similarly, on Day 7 after sciatic nerve axotomy, the amount of Nav1.1 protein and the percentage of Nav1.1-positive cells in the ipsilateral L5 DRG were also significantly increased. Our results suggest that an early increase in DRG Nav1.1 expression after peripheral nerve injury might be involved in the induction of neuropathic pain.
authors.affiliation: Department of Anesthesiology and Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Fidelis
authors.initials: F
authors.lastname: Atianjoh
authors.forename: Estelle B
authors.initials: EB
authors.lastname: Gauda
authors.forename: Myron
authors.initials: M
authors.lastname: Yaster
authors.forename: Yunqing
authors.initials: Y
authors.lastname: Li
authors.forename: Yuan-Xiang
authors.initials: YX
authors.lastname: Tao
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D015153:Blotting, Western; D004195:Disease Models, Animal; D005727:Ganglia, Spinal / Q000378:metabolism* / Q000503:physiopathology / Q000601:surgery; D007150:Immunohistochemistry; D008026:Ligation; D008297:Male; D062550:NAV1.1 Voltage-Gated Sodium Channel; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009437:Neuralgia / Q000378:metabolism* / Q000503:physiopathology; D010147:Pain Measurement; D017288:Pain Threshold; D059348:Peripheral Nerve Injuries / Q000378:metabolism* / Q000503:physiopathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000378:metabolism / Q000601:surgery; D015222:Sodium Channels / Q000378:metabolism*; D013127:Spinal Nerves / Q000378:metabolism* / Q000503:physiopathology / Q000601:surgery; D013997:Time Factors; D015854:Up-Regulation
chemical_list: D062550:NAV1.1 Voltage-Gated Sodium Channel; D009419:Nerve Tissue Proteins; C568250:Scn1a protein, rat; D015222:Sodium Channels