title: Effects of KRAS mutation and polymorphism on the risk and prognosis of oral squamous cell carcinoma.
abstract: BACKGROUND
  Mutations or single nucleotide polymorphism (SNP) of relevant genes may affect the risk and prognosis of malignancies. The purpose of this study was to analyze whether the KRAS polymorphisms and mutations can be useful prognostic or risk markers in oral squamous cell carcinoma (OSCC).
  
  
  METHODS
  DNA was extracted from tumor tissues of 47 patients with OSCC and blood cells of 84 normal controls and subjected to sequencing for the KRAS.
  
  
  RESULTS
  No mutation in the KRAS was found in 47 OSCC samples. However, 2 polymorphisms (rs1137282 and rs712) were detected. Individuals with KRAS SNP rs712 genotypes of G/T or T/T have a reduced risk for OSCC than those with genotype G/G (hazard ratio [HR], 0.26; 95% confidence interval [CI], 0.10-0.60; p = .004). The overall survival between different SNPs were not statistically significant (p = .147 for rs1137282 and p = .202 for rs712).
  
  
  CONCLUSIONS
  These data demonstrate a role for rs712 polymorphism of the KRAS in susceptibility of OSCC.
authors.affiliation: Section of Basic Medicine, Department of Nursing, Hung Kuang University, Taichung, Taiwan.
authors.forename: Wen-Yi
authors.initials: WY
authors.lastname: Wang
authors.forename: Yi-Chih
authors.initials: YC
authors.lastname: Chien
authors.forename: Yong-Kie
authors.initials: YK
authors.lastname: Wong
authors.forename: Yan-Liang
authors.initials: YL
authors.lastname: Lin
authors.forename: Jin-Ching
authors.initials: JC
authors.lastname: Lin
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000401:mortality; D016022:Case-Control Studies; D017931:DNA Primers; D005091:Exons; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics* / Q000401:mortality; D009154:Mutation; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016283:Proto-Oncogene Proteins p21(ras); D018631:ras Proteins / Q000235:genetics*
chemical_list: D017931:DNA Primers; C117307:KRAS protein, human; D011518:Proto-Oncogene Proteins; D016283:Proto-Oncogene Proteins p21(ras); D018631:ras Proteins