title: Identification of scavenger receptor ligands.
abstract: Scavenger receptors (SRs) are structurally diverse but functionally related innate immune receptors involved in defence and clearance mechanisms. Their broad specificity relies on evolutionarily conserved pattern recognition domains which interact with a variety of microbial, apoptotic and modified self ligands. Studies of immune functions of SR-expressing cells require the identification of interaction partners for SRs. We have developed an ELISA-based method which allows for large-scale high-throughput screening of complex mixtures. The assay successfully identified bacterial and plasma ligands for macrophage scavenger receptor A and can be adapted to screen for novel exogenous or endogenous ligands for any immune receptor of interest.
authors.affiliation: Department of Biochemistry, Sir William Dunn School of Pathology, University of Oxford, Oxford, UK.
authors.forename: Claudine
authors.initials: C
authors.lastname: Neyen
authors.forename: Annette
authors.initials: A
authors.lastname: Pluddemann
authors.forename: Siamon
authors.initials: S
authors.lastname: Gordon
mesh_terms: D000818:Animals; D015153:Blotting, Western; D016466:CHO Cells; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008024:Ligands; D011485:Protein Binding; D051116:Receptors, Scavenger / Q000378:metabolism*; D051117:Scavenger Receptors, Class A / Q000378:metabolism
chemical_list: D008024:Ligands; D051116:Receptors, Scavenger; D051117:Scavenger Receptors, Class A