title: Fibronectin layers by matrix-assisted pulsed laser evaporation from saline buffer-based cryogenic targets.
abstract: The deposition of fibronectin (FN) from saline buffer-based cryogenic targets by matrix-assisted pulsed laser evaporation (MAPLE) onto silicon substrates is reported. A uniform distribution of FN was revealed by Ponceau staining after control experiments on nitrocellulose paper. Well-organized particulates with heights from hundreds of nanometers up to more than 1 μm packed in homogeneous layers were evidenced by optical microscopy and profilometry on Si substrates. Atomic force microscopy images showed regions composed of buffer and FN aggregates forming a compact film. Comparison of infrared spectra of drop-cast and MAPLE-deposited FN confirmed the preservation of composition and showed no degradation of the protein. The protein deposition on Si was confirmed by antibody staining. Small aggregates and fluorescent fibrils were visualized by fluorescence microscopy. Superior attachment of human osteoprogenitor cells cultivated for 3 h proved the presence of stable and intact FN molecules after transfer.
authors.affiliation: National Institute for Lasers, Plasma and Radiation Physics, Bucharest, Romania.
authors.forename: F
authors.initials: F
authors.lastname: Sima
authors.forename: P
authors.initials: P
authors.lastname: Davidson
authors.forename: E
authors.initials: E
authors.lastname: Pauthe
authors.forename: L E
authors.initials: LE
authors.lastname: Sima
authors.forename: O
authors.initials: O
authors.lastname: Gallet
authors.forename: I N
authors.initials: IN
authors.lastname: Mihailescu
authors.forename: K
authors.initials: K
authors.lastname: Anselme
mesh_terms: D002021:Buffers; D002448:Cell Adhesion; D003080:Cold Temperature; D015925:Cryopreservation / Q000379:methods*; D005353:Fibronectins / Q000737:chemistry*; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D006801:Humans; D007834:Lasers; D018625:Microscopy, Atomic Force; D012965:Sodium Chloride / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D014835:Volatilization
chemical_list: D002021:Buffers; D005353:Fibronectins; D012965:Sodium Chloride; D016650:Fluorescein-5-isothiocyanate