title: The role of nanofibrous structure in osteogenic differentiation of human mesenchymal stem cells with serial passage.
abstract: Using scaffolds with autologous stem cells is a golden strategy for the treatment of bone defects. In this strategy, human mesenchymal stem cells (hMSCs) have often been isolated and expanded in vitro on a plastic surface to obtain a sufficient cell number before seeding on a suitable scaffold.
  
  
  METHODS
  Investigating the influence of serial passages (from passage two to passage eight) on the abilities of proliferation and osteogenic differentiation of hMSCs on 24-well tissue culture polystyrene plates and poly L-lactic acid electrospun nanofibrous scaffolds was performed to determine how prolonged culture affected these cellular abilities and how the nanofibrous scaffolds supported the osteogenic differentiation potential of hMSCs.
  
  
  CONCLUSIONS
  Serial passage caused adverse changes in hMSCs characteristics, which were indicated by the decline in both proliferation and osteogenic differentiation abilities. Interestingly, the poly L-lactic acid nanofibrous scaffolds showed a significant support in recovering the osteogenic abilities of hMSCs, which had been severely affected by prolonged culture.
authors.affiliation: National University of Singapore, 117576 Singapore. hienluong@nus.edu.sg
authors.forename: Luong T H
authors.initials: LT
authors.lastname: Nguyen
authors.forename: Susan
authors.initials: S
authors.lastname: Liao
authors.forename: Seeram
authors.initials: S
authors.lastname: Ramakrishna
authors.forename: Casey K
authors.initials: CK
authors.lastname: Chan
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D010012:Osteogenesis / Q000502:physiology*; D011091:Polyesters; D011108:Polymers / Q000737:chemistry; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D000469:Alkaline Phosphatase; D002118:Calcium