title: The PPR-DYW proteins are required for RNA editing of rps14, cox1 and nad5 transcripts in Physcomitrella patens mitochondria.
abstract: We identified two DYW subclass pentatricopeptide repeat (PPR) proteins, PpPPR_78 and PpPPR_79, as RNA editing factors in the moss Physcomitrella patens. Disruption of each gene by homologous recombination revealed that PpPPR_78 was involved in RNA editing at the rps14 (rps14-C137) and cox1 (cox1-C755) sites and PpPPR_79 at the nad5-1 (nad5-C598) site in the mitochondrial transcripts. RNA editing defects did not affect transcript patterns of the target genes. Thus, DYW subclass PPR proteins seem to be site-specific trans-acting factors for RNA editing.
authors.affiliation: Center for Gene Research, Nagoya University, Chikusa-ku, Nagoya, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Uchida
authors.forename: Shotaro
authors.initials: S
authors.lastname: Ohtani
authors.forename: Mizuho
authors.initials: M
authors.lastname: Ichinose
authors.forename: Chieko
authors.initials: C
authors.lastname: Sugita
authors.forename: Mamoru
authors.initials: M
authors.lastname: Sugita
mesh_terms: D019068:Bryopsida / Q000235:genetics* / Q000378:metabolism*; D051545:Cyclooxygenase 1 / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D017393:RNA Editing; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012269:Ribosomal Proteins / Q000235:genetics / Q000378:metabolism*; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism
chemical_list: D024101:Mitochondrial Proteins; D010940:Plant Proteins; D018749:RNA, Plant; D011993:Recombinant Fusion Proteins; D012269:Ribosomal Proteins; D015534:Trans-Activators; D051545:Cyclooxygenase 1