title: Bisphenol A and estrone-induced developmental effects in early chick embryos.
abstract: Endocrine disruptors, especially estrogenic substances, are thought to affect the reproduction and development of animals, including humans. We therefore assessed whether bisphenol A (BPA) or estrone (E1) had any adverse effects on chick embryogenesis. Fertilized eggs of white Leghorns were obtained within 24 h after laying. Embryos were administered 0.1, 1.0, and 10.0 mM BPA, and administered 10, 100 nM, and 1 μM E1, and incubated for 48 h at 37 ± 0.5°C and >80% relative humidity with one rotation per hour. The embryos were excised, fixed in 70% ethanol and viewed under a stereomicroscope. Their morphological abnormalities and numbers of somites were recorded. There were no significant difference in the average number of somites in embryos administered BPA and controls. Abnormal embryogenesis, however, showed dose-related increases caused by BPA and E1.
authors.affiliation: Laboratory of Environmental Health and Food Science, Nippon Veterinary and Life Science University, Musashino, Tokyo, Japan. k-saito@nvlu.ac.jp
authors.forename: Kenichi
authors.initials: K
authors.lastname: Saito
authors.forename: Azusa
authors.initials: A
authors.lastname: Niijima
authors.forename: Eri
authors.initials: E
authors.lastname: Kamite
authors.forename: Mari
authors.initials: M
authors.lastname: Watanabe
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds; D002642:Chick Embryo; D047108:Embryonic Development / Q000187:drug effects; D052244:Endocrine Disruptors / Q000633:toxicity*; D004970:Estrone / Q000633:toxicity*; D006801:Humans; D010636:Phenols / Q000633:toxicity*; D015053:Zygote / Q000187:drug effects / Q000254:growth & development; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D052244:Endocrine Disruptors; D004970:Estrone; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds