title: A rapid PCR-RFLP method for monitoring genetic variation among commercial mushroom species*.
abstract: We report the development of a simplified procedure for restriction fragment length polymorphism (RFLP) analysis of mushrooms. We have adapted standard molecular techniques to be amenable to an undergraduate laboratory setting in order to allow students to explore basic questions about fungal diversity and relatedness among mushroom species. The streamlined protocols allowed students to practice important molecular techniques within the context of self-designed investigative projects. This laboratory experience provided opportunities for students to practice strategies for examining molecular diversity among species.
authors.affiliation: Biology Department, Hamline University, St. Paul, MN 55104.
authors.forename: Presley
authors.initials: P
authors.lastname: Martin
authors.forename: Masoud
authors.initials: M
authors.lastname: Muruke
authors.forename: Kenneth
authors.initials: K
authors.lastname: Hosea
authors.forename: Amelia
authors.initials: A
authors.lastname: Kivaisi
authors.forename: Nick
authors.initials: N
authors.lastname: Zerwas
authors.forename: Cynthia
authors.initials: C
authors.lastname: Bauerle