title: Branched RNA nanostructures for RNA interference.
abstract: Branched RNAs with three- or four-way junctions were designed by assembling single-stranded RNA for RNA interference. Human Dicer transformed branched RNAs into about 20 base pairs of double-stranded RNA, which is a standard siRNA species. Our tetramer design provides a potent silencing effect over a period of 5 days.
authors.affiliation: Nano Medical Engineering Laboratory, RIKEN Advanced Science Institute, 2-1, Hirosawa, Wako-Shi, Saitama, 351-0198, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Nakashima
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Abe
authors.forename: Naoko
authors.initials: N
authors.lastname: Abe
authors.forename: Kyoko
authors.initials: K
authors.lastname: Aikawa
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Ito
mesh_terms: D053487:DEAD-box RNA Helicases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D049329:Nanostructures / Q000737:chemistry*; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000737:chemistry* / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D043244:Ribonuclease III / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism
chemical_list: D012330:RNA, Double-Stranded; D034741:RNA, Small Interfering; C408087:DICER1 protein, human; D043244:Ribonuclease III; D053487:DEAD-box RNA Helicases