title: Sequencing technologies and genome sequencing.
abstract: The high-throughput - next generation sequencing (HT-NGS) technologies are currently the hottest topic in the field of human and animals genomics researches, which can produce over 100 times more data compared to the most sophisticated capillary sequencers based on the Sanger method. With the ongoing developments of high throughput sequencing machines and advancement of modern bioinformatics tools at unprecedented pace, the target goal of sequencing individual genomes of living organism at a cost of $1,000 each is seemed to be realistically feasible in the near future. In the relatively short time frame since 2005, the HT-NGS technologies are revolutionizing the human and animal genome researches by analysis of chromatin immunoprecipitation coupled to DNA microarray (ChIP-chip) or sequencing (ChIP-seq), RNA sequencing (RNA-seq), whole genome genotyping, genome wide structural variation, de novo assembling and re-assembling of genome, mutation detection and carrier screening, detection of inherited disorders and complex human diseases, DNA library preparation, paired ends and genomic captures, sequencing of mitochondrial genome and personal genomics. In this review, we addressed the important features of HT-NGS like, first generation DNA sequencers, birth of HT-NGS, second generation HT-NGS platforms, third generation HT-NGS platforms: including single molecule Heliscope™, SMRT™ and RNAP sequencers, Nanopore, Archon Genomics X PRIZE foundation, comparison of second and third HT-NGS platforms, applications, advances and future perspectives of sequencing technologies on human and animal genome research.
authors.affiliation: Laboratory of Functional Genomics, Institute of General and Molecular Biology, Nicolaus Copernicus University, Torun, Poland. pareekcs@umk.pl
authors.forename: Chandra Shekhar
authors.initials: CS
authors.lastname: Pareek
authors.forename: Rafal
authors.initials: R
authors.lastname: Smoczynski
authors.forename: Andrzej
authors.initials: A
authors.lastname: Tretyn
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000235:genetics; D057890:Epigenomics; D014644:Genetic Variation; D016678:Genome; D059014:High-Throughput Nucleotide Sequencing / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D009154:Mutation; D009369:Neoplasms / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000295:instrumentation / Q000379:methods*; D017423:Sequence Analysis, RNA / Q000379:methods
chemical_list: D014408:Biomarkers, Tumor