title: Tumor necrosis factor alpha promoter polymorphisms in Mexican patients with dengue fever.
abstract: Increased levels of tumor necrosis factor alpha (TNF-α) in patients with dengue have been reported. Various polymorphisms have been identified in the promoter region of the TNF-α gene that may affect its transcription. The purpose of the present study was to evaluate the relationship between polymorphisms of TNF-α gene and the genetic susceptibility to dengue fever in a group of patients from Morelos State, Mexico. The TNF-α polymorphisms (positions -238 and -308) were determined by PCR-RFLP technique in 130 patients with dengue (85 with dengue fever and 45 with dengue hemorrhagic fever) and 169 healthy controls. The patients were selected from cases reported in Morelos State from 1997 to 2003. The whole group of dengue patients showed a decreased frequency of TNF-α -238 A allele when compared to healthy controls (p = 0.01, OR = 0.19, 95%CI = 0.02-0.78). When the analysis was made separately in dengue fever and dengue hemorrhagic fever patients, the decreased frequency of TNF-α -238 A allele only remained significant in patients with DHF when compared to healthy controls (p = 0.034). This work suggests a possible association of TNF-α -238 A allele with protection to develop symptomatic disease.
authors.affiliation: Center for Infectious Disease Research, Instituto Nacional de Salud Publica, Cuernavaca, Morelos, Mexico.
authors.forename: Alma Rosa
authors.initials: AR
authors.lastname: García-Trejo
authors.forename: Jorge A
authors.initials: JA
authors.lastname: Falcón-Lezama
authors.forename: Lilia
authors.initials: L
authors.lastname: Juárez-Palma
authors.forename: Julio
authors.initials: J
authors.lastname: Granados
authors.forename: Joaquín
authors.initials: J
authors.lastname: Zúñiga-Ramos
authors.forename: Hilda
authors.initials: H
authors.lastname: Rangel
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Barquera
authors.forename: Gilberto
authors.initials: G
authors.lastname: Vargas-Alarcón
authors.forename: Celso
authors.initials: C
authors.lastname: Ramos
mesh_terms: D000483:Alleles; D016022:Case-Control Studies; D003716:Dengue Virus / Q000276:immunology / Q000472:pathogenicity; D020022:Genetic Predisposition to Disease; D006801:Humans; D008800:Mexico / Q000453:epidemiology; D011110:Polymorphism, Genetic; D019595:Severe Dengue / Q000235:genetics / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000276:immunology
chemical_list: D014409:Tumor Necrosis Factor-alpha