title: A validated hybrid computational fluid dynamics-physiologically based pharmacokinetic model for respiratory tract vapor absorption in the human and rat and its application to inhalation dosimetry of diacetyl.
abstract: Diacetyl vapor is associated with bronchiolar injury in man but primarily large airway injury in the rat. The goal of this study was to develop a physiologically based pharmacokinetic model for inspired vapor dosimetry and to apply the model to diacetyl. The respiratory tract was modeled as a series of airways: nose, trachea, main bronchi, large bronchi, small bronchi, bronchioles, and alveoli with tissue dimensions obtained from the literature. Airborne vapor was allowed to absorb (or desorb) from tissues based on mass transfer coefficients. Transfer of vapor within tissues was based on molecular diffusivity with direct reaction with tissue substrates and/or metabolism being allowed in each tissue compartment. In vitro studies were performed to provide measures of diacetyl metabolism kinetics and direct reaction rates allowing for the development of a model with no unassigned variables. Respiratory tract uptake of halothane, acetone, ethanol and diacetyl was measured in male F344 rat to obtain data for model validation. The human model was validated against published values for inspired vapor uptake. For both the human and rat models, a close concordance of model estimates with experimental measurements was observed, validating the model. The model estimates that limited amounts of inspired diacetyl penetrate to the bronchioles of the rat (<2%), whereas in the lightly exercising human, 24% penetration to the bronchioles is estimated. Bronchiolar tissue concentrations of diacetyl in the human are estimated to exceed those in the rat by 40-fold. These inhalation dosimetric differences may contribute to the human-rat differences in diacetyl-induced airway injury.
authors.affiliation: Department of Pharmaceutical Sciences, Toxicology Program, University of Connecticut, Storrs, Connecticut 06269-3092, USA.
authors.forename: Eric
authors.initials: E
authors.lastname: Gloede
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Cichocki
authors.forename: Joshua B
authors.initials: JB
authors.lastname: Baldino
authors.forename: John B
authors.initials: JB
authors.lastname: Morris
mesh_terms: D000818:Animals; D003198:Computer Simulation; D003931:Diacetyl / Q000008:administration & dosage / Q000493:pharmacokinetics*; D004305:Dose-Response Relationship, Drug; D005740:Gases / Q000008:administration & dosage / Q000493:pharmacokinetics*; D006801:Humans; D019570:Inhalation Exposure / Q000032:analysis*; D008297:Male; D008954:Models, Biological; D051381:Rats; D011916:Rats, Inbred F344; D020545:Respiratory Mucosa / Q000187:drug effects / Q000378:metabolism*; D014835:Volatilization
chemical_list: D005740:Gases; D003931:Diacetyl