title: The use of pulsed-field gel electrophoresis to investigate the epidemiology of Mycoplasma bovis in French calf feedlots.
abstract: Mycoplasma bovis is a major cause of respiratory outbreaks in cattle feedlots. In this study pulsed-field gel electrophoresis (PFGE) was used to trace field strains and provide information on M. bovis patterns of spread in calf feedlots. The suitability of KpnI, MluI and SmaI restriction enzymes was assessed on different sets of strains. The discriminative power of the first two enzymes was first assessed using 28 epidemiologically unrelated strains; stability was 100% on multiple isolates from in vivo experimental infection. Thirty-nine field isolates from six feedlots were then evaluated. In contrast to the unique fingerprints displayed by the unrelated strains, the isolates from the feedlots showed identical patterns at the time of the outbreak of respiratory disease and 4 weeks later. The PFGE typing results suggest that M. bovis strains follow a clonal epidemic spread pattern at the herd level and that the same strain persists in calves of the herd after the clinical signs have disappeared.
authors.affiliation: Université de Lyon, Lyon F-69003, France. ma.arcangioli@vetagro-sup.fr
authors.forename: Marie-Anne
authors.initials: MA
authors.lastname: Arcangioli
authors.forename: Hamidé
authors.initials: H
authors.lastname: Aslan
authors.forename: Florence
authors.initials: F
authors.lastname: Tardy
authors.forename: François
authors.initials: F
authors.lastname: Poumarat
authors.forename: Dominique
authors.initials: D
authors.lastname: Le Grand
mesh_terms: D000818:Animals; D048090:Bovine Respiratory Disease Complex / Q000453:epidemiology* / Q000382:microbiology; D002417:Cattle; D004262:DNA Restriction Enzymes / Q000032:analysis* / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics*; D004196:Disease Outbreaks / Q000662:veterinary*; D016521:Electrophoresis, Gel, Pulsed-Field / Q000662:veterinary; D005602:France / Q000453:epidemiology; D009175:Mycoplasma Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D045682:Mycoplasma bovis / Q000145:classification* / Q000235:genetics; D010802:Phylogeny
chemical_list: D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes