title: Association of GSTM1 and GSTT1 polymorphism with lipid peroxidation in benign prostate hyperplasia and prostate cancer: a pilot study.
abstract: Association of glutathione S-transferase (GST) M1 and T1 deletions with benign prostate hyperplasia (BPH) and prostate cancer is well reported. These enzymes metabolize numerous toxins thus protecting from oxidative injury. Oxidative stress has been associated with development of BPH and prostate cancer. The present study was designed to analyze role of GST deletions in development of oxidative stress in these subjects. GSTs are responsible for metabolism of toxins present in tobacco therefore effect of tobacco usage in study groups was also studied. Three groups of subjects: BPH (53 patients), prostate cancer (57 patients) and controls (46 subjects) were recruited [corrected]. Genotyping was done using a multiplex polymerase chain reaction (PCR) method. Malondialdehyde (MDA) levels as marker of oxidative stress were estimated by measuring thiobarbituric acid reactive substance (TBARS) in plasma. Based on genotyping, subjects were categorized into: GSTM1+/GSTT1+, GSTM1-/GSTT1+, GSTM1+/GSTT1- and GSTM1-/GSTT1-. Significantly higher plasma MDA levels were noticed in GSTM1-/GSTT1- as compared to GSTM1+/GSTT1+ in all study groups. Double deletion (GSTM1-/GSTT1-) is associated with higher oxidative stress which might play a role in the pathogenesis of BPH and prostate cancer. However, other markers of oxidative stress should be analyzed before any firm conclusion.
authors.affiliation: Department of Biochemistry, University College of Medical Sciences and GTB Hospital, University of Delhi, Dilshad Garden, Delhi, India.
authors.forename: Vivek
authors.initials: V
authors.lastname: Kumar
authors.forename: Chandra Shekhar
authors.initials: CS
authors.lastname: Yadav
authors.forename: Sudip Kumar
authors.initials: SK
authors.lastname: Datta
authors.forename: Satyender
authors.initials: S
authors.lastname: Singh
authors.forename: Rafat S
authors.initials: RS
authors.lastname: Ahmed
authors.forename: Sanjay
authors.initials: S
authors.lastname: Goel
authors.forename: Sanjay
authors.initials: S
authors.lastname: Gupta
authors.forename: Md
authors.initials: M
authors.lastname: Mustafa
authors.forename: Rajesh Kumar
authors.initials: RK
authors.lastname: Grover
authors.forename: Basu Dev
authors.initials: BD
authors.lastname: Banerjee
mesh_terms: D000368:Aged; D015415:Biomarkers / Q000097:blood; D003430:Cross-Sectional Studies; D005838:Genotype; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D015227:Lipid Peroxidation / Q000235:genetics*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D018384:Oxidative Stress / Q000235:genetics* / Q000502:physiology; D010865:Pilot Projects; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011470:Prostatic Hyperplasia / Q000235:genetics*; D011471:Prostatic Neoplasms / Q000235:genetics*; D017384:Sequence Deletion; D012907:Smoking; D014030:Tobacco, Smokeless
chemical_list: D015415:Biomarkers; D005982:Glutathione Transferase; D008315:Malondialdehyde; C413545:glutathione S-transferase T1