title: Occupational exposure to formaldehyde: genotoxic risk evaluation by comet assay and micronucleus test using human peripheral lymphocytes.
abstract: Formaldehyde (FA) is a world high-production compound with numerous applications ranging from production of resins to medicines. Due to its sensitizing properties, irritating effects and potential cancer hazard FA is of great environmental health concern. Numerous studies in humans and experimental animals demonstrated that inhaled FA produced toxicity, genotoxicity, and cancer at distal sites. IARC, based on sufficient data, reclassified FA as a human carcinogen. The highest level of human exposure to this aldehyde occurs in occupational settings, namely, in pathology and anatomy laboratories, where FA is commonly used as a fixative and tissue preservative. Several studies consistently showed that the levels of airborne FA in anatomy laboratories exceeded recommended exposure criteria. In order to assess the genotoxic effects of chronic occupational exposure to FA, a group of pathology/anatomy workers was assessed using a micronucleus (MN) test and comet assay. The level of exposure to FA was also determined and the time-weighted average (TWA) of exposure was calculated for each subject. The TWA mean value for FA exposed workers was 0.43 ± 0.06 ppm, exceeding national and international recommended limit levels of 0.3 ppm. Both MN frequency and comet assay parameters were significantly higher in exposed subjects. Data obtained confirm a correlation between genetic damage and occupational exposure to FA. These data, along with recent implications of human carcinogenicity, point out the need for close monitoring of occupational exposure to FA. Implementation of security and hygiene measures as well as good practices campaigns may be crucial to decrease risk.
authors.affiliation: National Institute of Health, Environmental Health Department, Porto, Portugal. solange.costa2@gmail.com
authors.forename: Solange
authors.initials: S
authors.lastname: Costa
authors.forename: Carolina
authors.initials: C
authors.lastname: Pina
authors.forename: Patrícia
authors.initials: P
authors.lastname: Coelho
authors.forename: Carla
authors.initials: C
authors.lastname: Costa
authors.forename: Susana
authors.initials: S
authors.lastname: Silva
authors.forename: Beatriz
authors.initials: B
authors.lastname: Porto
authors.forename: Blanca
authors.initials: B
authors.lastname: Laffon
authors.forename: João Paulo
authors.initials: JP
authors.lastname: Teixeira
mesh_terms: D000328:Adult; D020552:Comet Assay; D004781:Environmental Exposure; D005260:Female; D005557:Formaldehyde / Q000633:toxicity*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects*; D008297:Male; D015162:Micronucleus Tests; D008875:Middle Aged; D016273:Occupational Exposure; D011174:Portugal; D055815:Young Adult
chemical_list: D005557:Formaldehyde