title: A spectroscopic-based laboratory experiment for protein conformational studies*.
abstract: This article describes a practical experiment for teaching basic spectroscopic techniques to introduce the topic of protein conformational change to students in the field of molecular biology, biochemistry, or structural biology. The spectroscopic methods employed in the experiment are absorbance, for protein concentration measurements, and fluorescence and circular dichroism, for probing protein conformational changes.
authors.affiliation: Centro de Biologia Molecular Estrutural, Laboratório Nacional de Luz Síncrotron, CP 6192, Campinas SP, 13084-971 Brazil, and Departamento de Bioquímica, Instituto de Biologia, Universidade Estadual de Campinas, Campinas SP, Brazil. cramos@lnls.br.
authors.forename: Carlos Henrique I
authors.initials: CH
authors.lastname: Ramos