title: Flavonoid-DNA binding studies and thermodynamic parameters.
abstract: Interactional studies of new flavonoid derivatives (Fl) with chicken blood ds.DNA were investigated spectrophotometrically in DMSO-H2O (9:1 v/v) at various temperatures. Spectral parameters suggest considerable binding between the flavonoid derivatives studied and ds.DNA. The binding constant values lie in the enhanced-binding range. Thermodynamic parameters obtained from UV studies also point to strong spontaneous binding of Fl with ds.DNA. Viscometric studies complimented the UV results where a small linear increase in relative viscosity of the DNA solution was observed with added optimal flavonoid concentration. An overall mixed mode of interaction (intercalative plus groove binding) is proposed between DNA and flavonoids. Conclusively, investigated flavonoid derivatives are found to be strong DNA binders and seem to be promising drug candidates like their natural analogues.
authors.affiliation: Department of Chemistry, Quaid-i-Azam University, Islamabad 45230, Pakistan. nkausarjanjua@yahoo.com
authors.forename: Naveed Kausar
authors.initials: NK
authors.lastname: Janjua
authors.forename: Amber
authors.initials: A
authors.lastname: Shaheen
authors.forename: Azra
authors.initials: A
authors.lastname: Yaqub
authors.forename: Fouzia
authors.initials: F
authors.lastname: Perveen
authors.forename: Sana
authors.initials: S
authors.lastname: Sabahat
authors.forename: Misbah
authors.initials: M
authors.lastname: Mumtaz
authors.forename: Claus
authors.initials: C
authors.lastname: Jacob
authors.forename: Lalla Aicha
authors.initials: LA
authors.lastname: Ba
authors.forename: Hamdoon A
authors.initials: HA
authors.lastname: Mohammed
mesh_terms: D000818:Animals; D002645:Chickens; D004247:DNA / Q000378:metabolism*; D005419:Flavonoids / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D013816:Thermodynamics
chemical_list: D005419:Flavonoids; D004247:DNA