title: Treatment of newborn G6pc(-/-) mice with bone marrow-derived myelomonocytes induces liver repair.
abstract: OBJECTIVE
  Several studies have shown that bone marrow-derived committed myelomonocytic cells can repopulate diseased livers by fusing with host hepatocytes and can restore normal liver function. These data suggest that myelomonocyte transplantation could be a promising approach for targeted and well-tolerated cell therapy aimed at liver regeneration. We sought to determine whether bone marrow-derived myelomonocytic cells could be effective for liver reconstitution in newborn mice knock-out for glucose-6-phosphatase-α.
  
  
  METHODS
  Bone marrow-derived myelomonocytic cells obtained from adult wild type mice were transplanted in newborn knock-out mice. Tissues of control and treated mice were frozen for histochemical analysis, or paraffin-embedded and stained with hematoxylin and eosin for histological examination or analyzed by immunohistochemistry or fluorescent in situ hybridization.
  
  
  RESULTS
  Histological sections of livers of treated knock-out mice revealed areas of regenerating tissue consisting of hepatocytes of normal appearance and partial recovery of normal architecture as early as 1 week after myelomonocytic cells transplant. FISH analysis with X and Y chromosome paints indicated fusion between infused cells and host hepatocytes. Glucose-6-phosphatase activity was detected in treated mice with improved profiles of liver functional parameters.
  
  
  CONCLUSIONS
  Our data indicate that bone marrow-derived myelomonocytic cell transplant may represent an effective way to achieve liver reconstitution of highly degenerated livers in newborn animals.
authors.affiliation: Laboratory of Molecular Biology, G Gaslini Institute, Genova, Italy.
authors.forename: Roberta
authors.initials: R
authors.lastname: Resaz
authors.forename: Laura
authors.initials: L
authors.lastname: Emionite
authors.forename: Cristina
authors.initials: C
authors.lastname: Vanni
authors.forename: Simonetta
authors.initials: S
authors.lastname: Astigiano
authors.forename: Maura
authors.initials: M
authors.lastname: Puppo
authors.forename: Rosa
authors.initials: R
authors.lastname: Lavieri
authors.forename: Daniela
authors.initials: D
authors.lastname: Segalerba
authors.forename: Annalisa
authors.initials: A
authors.lastname: Pezzolo
authors.forename: Maria Carla
authors.initials: MC
authors.lastname: Bosco
authors.forename: Alessandra
authors.initials: A
authors.lastname: Oberto
authors.forename: Carola
authors.initials: C
authors.lastname: Eva
authors.forename: Janice Y
authors.initials: JY
authors.lastname: Chou
authors.forename: Luigi
authors.initials: L
authors.lastname: Varesio
authors.forename: Ottavia
authors.initials: O
authors.lastname: Barbieri
authors.forename: Alessandra
authors.initials: A
authors.lastname: Eva
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D016026:Bone Marrow Transplantation; D005260:Female; D005952:Glucose-6-Phosphatase / Q000235:genetics / Q000378:metabolism; D005953:Glycogen Storage Disease Type I / Q000235:genetics / Q000473:pathology* / Q000503:physiopathology / Q000628:therapy*; D008099:Liver / Q000473:pathology* / Q000503:physiopathology; D008115:Liver Regeneration; D008297:Male; D051379:Mice; D020297:Mice, Congenic; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D022423:Myeloid Cells / Q000637:transplantation*; D011247:Pregnancy
chemical_list: D005952:Glucose-6-Phosphatase