title: Gene delivery to tumor cells by cationic polymeric nanovectors coupled to folic acid and the cell-penetrating peptide octaarginine.
abstract: Target ligand folic acid (FA) and cell-penetrating peptide octaarginine (R8) were coupled with the gene vectors (PEI(600)-CyD, PC) composed of β-cyclodextrin (β-CyD) and low-molecular-weight polyethylenimine (PEI, Mw 600) to form nanovectors for highly efficient gene delivery to tumor cells. The resultant ternary nanocomplexes of FA-PC/R8-PC/pDNA produced excellent gene transfaction abilities in the folate receptor (FR)-positive tumor cells in vitro and in vivo. The FR-mediated endocytosis and the R8-mediated transmembrane functionality together contributed to the high transfection levels. This study provides a promising means to produce gene nanovectors for in vivo applications.
authors.affiliation: Institute of Chemical Biology and Pharmaceutical Chemistry, Zhejiang University, Hangzhou, China.
authors.forename: Qi-Ying
authors.initials: QY
authors.lastname: Jiang
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Lai
authors.forename: Jie
authors.initials: J
authors.lastname: Shen
authors.forename: Qing-Qing
authors.initials: QQ
authors.lastname: Wang
authors.forename: Fu-Jian
authors.initials: FJ
authors.lastname: Xu
authors.forename: Gu-Ping
authors.initials: GP
authors.lastname: Tang
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D002412:Cations / Q000737:chemistry*; D002460:Cell Line; D057846:Cell-Penetrating Peptides / Q000737:chemistry / Q000378:metabolism; D005492:Folic Acid / Q000737:chemistry*; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism* / Q000633:toxicity; D006801:Humans; D008422:Materials Testing; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D015394:Molecular Structure; D049329:Nanostructures / Q000737:chemistry* / Q000633:toxicity; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism*; D011108:Polymers / Q000737:chemistry* / Q000633:toxicity; D014162:Transfection
chemical_list: D001672:Biocompatible Materials; D002412:Cations; D057846:Cell-Penetrating Peptides; D009842:Oligopeptides; D011108:Polymers; C448619:octaarginine; D005492:Folic Acid